title: Effect of β-lactam antibiotics on plant regeneration in carrot protoplast cultures.
abstract: Protoplasts of three carrot cultivars were isolated fromin vitro-grown plantlets by overnight incubation in an enzyme mixture composed of 1% (w/v) cellulase Onozuka R-10 and 0.1% (w/v) pectolyase Y-23. After cell immobilization in modified thin alginate layers, three types of β-lactam antibiotics (cefotaxime, carbenicillin, or timentin) at five different concentrations (100, 200, 300, 400, or 500 mg L-1) were added to the culture medium. In 20-d-old cultures, a different number of cell colonies had formed and varied on average from 27 to 56% in carbenicillin- and cefotaxime-containing media, respectively. Supplementation of the culture media with antibiotics at concentrations higher than 100 mg L-1resulted in a decrease in plating efficiency in comparison with the controls. However, from all antibiotic treatments, except carbenicillin at concentrations of 400-500 mg L-1, efficient plant regeneration occurred. For this reason, we believe that cefotaxime and timentin in the concentrations analyzed here may be used in complexin vitroprocedures or valuable carrot cultures as a prophylactic agent for prevention against occasional contaminations.
authors.affiliation: Institute of Plant Biology and Biotechnology, Faculty of Horticulture, University of Agriculture in Krakow, Al. 29 Listopada 54, 31-425 Krakow, Poland.
authors.forename: Ewa
authors.initials: E
authors.lastname: Grzebelus
authors.affiliation: Institute of Plant Biology and Biotechnology, Faculty of Horticulture, University of Agriculture in Krakow, Al. 29 Listopada 54, 31-425 Krakow, Poland.
authors.forename: Lukasz
authors.initials: L
authors.lastname: Skop
keywords: Carbenicillin; Cefotaxime; Daucus carota; Somatic embryogenesis; Timentin