title: Activation of BNGR-A24 by direct interaction with tachykinin-related peptides from the silkworm Bombyx mori leads to the Gq- and Gs-coupled signaling cascades.
abstract: Tachykinins constitute one of the largest peptide families in the animal kingdom and exert their diverse actions via G protein-coupled receptors (GPCRs). In this study, the Bombyx tachykinin-related peptides (TKRPs) were identified as specific endogenous ligands for the Bombyx neuropeptide GPCR A24 (BNGR-A24) and thus designated BNGR-A24 as BmTKRPR. Using both mammalian cell line HEK293 and insect cell line Sf21, further characterization demonstrated that BmTKRPR was activated, thus resulting in intracellular accumulation of cAMP, Ca(2+) mobilization, and ERK1/2 phosphorylation in a Gs and Gq inhibitor-sensitive manner. Moreover, quantitative reverse transcriptase polymerase chain reaction analysis and dsRNA-mediated knockdown experiments suggested a possible role for BmTKRPR in the regulation of feeding and growth. Our findings enhance the understanding of the Bombyx TKRP system in the regulation of fundamental physiological processes.
authors.affiliation: Institute of Biochemistry, College of Life Sciences, Zhejiang University , Zijingang Campus, Hangzhou, Zhejiang 310058, China.
authors.forename: Xiaobai
authors.initials: X
authors.lastname: He
authors.forename: Jiashu
authors.initials: J
authors.lastname: Zang
authors.forename: Xiangmei
authors.initials: X
authors.lastname: Li
authors.forename: Jiajie
authors.initials: J
authors.lastname: Shao
authors.forename: Huipeng
authors.initials: H
authors.lastname: Yang
authors.forename: Jingwen
authors.initials: J
authors.lastname: Yang
authors.forename: Haishan
authors.initials: H
authors.lastname: Huang
authors.forename: Linjie
authors.initials: L
authors.lastname: Chen
authors.forename: Liangen
authors.initials: L
authors.lastname: Shi
authors.forename: Chenggang
authors.initials: C
authors.lastname: Zhu
authors.forename: Guozheng
authors.initials: G
authors.lastname: Zhang
authors.forename: Naiming
authors.initials: N
authors.lastname: Zhou
mesh_terms: D000818:Animals; D012831:Bombyx / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism; D003001:Cloning, Molecular; D000242:Cyclic AMP / Q000096:biosynthesis; D043802:GTP-Binding Protein alpha Subunits, Gq-G11 / Q000378:metabolism*; D019205:GTP-Binding Protein alpha Subunits, Gs / Q000378:metabolism*; D057809:HEK293 Cells; D006801:Humans; D008024:Ligands; D019950:Mitogen-Activated Protein Kinase 1 / Q000378:metabolism; D048052:Mitogen-Activated Protein Kinase 3 / Q000378:metabolism; D009479:Neuropeptides / Q000378:metabolism*; D010766:Phosphorylation; D018039:Receptors, Tachykinin / Q000235:genetics / Q000378:metabolism*; D061987:Sf9 Cells; D015398:Signal Transduction; D015320:Tachykinins / Q000378:metabolism*
chemical_list: D008024:Ligands; D009479:Neuropeptides; D018039:Receptors, Tachykinin; D015320:Tachykinins; D000242:Cyclic AMP; D019950:Mitogen-Activated Protein Kinase 1; D048052:Mitogen-Activated Protein Kinase 3; D043802:GTP-Binding Protein alpha Subunits, Gq-G11; D019205:GTP-Binding Protein alpha Subunits, Gs; D002118:Calcium