title: Impaired glucose tolerance alters functional ability of peripheral blood-derived mononuclear cells in Asian Indian men.
abstract: OBJECTIVE
  To compare the adhesion, migration and endothelial differentiation potential of peripheral blood-derived mononuclear cells (PBMCs) obtained from drug-naive normal glucose tolerance (NGT) and impaired glucose tolerance (IGT) Asian Indian men.
  
  
  METHODS
  Based on the 75-g oral glucose tolerance test, 30 NGT and 31 IGT subjects were recruited into the study. PBMCs were isolated from fasting blood using histopaque density gradient centrifugation. Isolated PBMCs were analysed for their ability to adhere to extracellular matrices, incorporation into tubular structures formed by matured endothelial cells and differentiation into endothelial cells upon 7-day culture in endothelial-specific growth medium.
  
  
  RESULTS
  PBMCs obtained from IGT subjects exhibit poor adherence to fibronectin and reduced incorporation into tubular structures. Migration towards stromal cell-derived factor-1α (SDF-1α) in a trans-well filter assembly was also reduced for these cells. Semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) analysis revealed decreased expression of CXCR4 and β2 integrin and increased expression of arginase II in IGT subjects. No differences were observed with regard to endothelial differentiation; however, cultured PBMCs of IGT subjects had decreased intracellular nitric oxide (NO) production.
  
  
  CONCLUSIONS
  In pre-diabetic, Asian Indian men, PBMCs exhibit defective migration and homing potential.
authors.affiliation: Department of Biotechnology, Bhupat & Jyoti Mehta School of Biosciences and Bioengineering, Indian Institute of Technology Madras, Chennai, India.
authors.forename: Abel Arul
authors.initials: AA
authors.lastname: Nathan
authors.affiliation: Department of Biotechnology, Bhupat & Jyoti Mehta School of Biosciences and Bioengineering, Indian Institute of Technology Madras, Chennai, India.
authors.forename: Mallu Abhiram Charan
authors.initials: MA
authors.lastname: Tej
authors.affiliation: Department of Biotechnology, Bhupat & Jyoti Mehta School of Biosciences and Bioengineering, Indian Institute of Technology Madras, Chennai, India.
authors.forename: Maneka
authors.initials: M
authors.lastname: Chitiprolu
authors.affiliation: Department of Biotechnology, Bhupat & Jyoti Mehta School of Biosciences and Bioengineering, Indian Institute of Technology Madras, Chennai, India.
authors.forename: Shreyas
authors.initials: S
authors.lastname: Rangan
authors.affiliation: Department of Diabetology, Madras Diabetes Research Foundation (MDRF) and Dr Mohan's Diabetes Specialties Centre, Gopalapuram, Chennai, India.
authors.forename: Viswanathan
authors.initials: V
authors.lastname: Mohan
authors.affiliation: Department of Diabetology, Madras Diabetes Research Foundation (MDRF) and Dr Mohan's Diabetes Specialties Centre, Gopalapuram, Chennai, India.
authors.forename: Ranjani
authors.initials: R
authors.lastname: Harish
authors.affiliation: Department of Genetic Engineering, Madurai Kamaraj University, Madurai, India.
authors.forename: Setty B
authors.initials: SB
authors.lastname: Anand
authors.affiliation: Department of Biotechnology, Bhupat & Jyoti Mehta School of Biosciences and Bioengineering, Indian Institute of Technology Madras, Chennai, India mdixit@iitm.ac.in.
authors.forename: Madhulika
authors.initials: M
authors.lastname: Dixit
mesh_terms: D000328:Adult; D001119:Arginase / Q000235:genetics / Q000378:metabolism; D044466:Asian People; D018821:CD18 Antigens / Q000235:genetics / Q000378:metabolism; D002448:Cell Adhesion; D002454:Cell Differentiation; D002465:Cell Movement; D054338:Cell Transdifferentiation; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000276:immunology / Q000473:pathology; D005109:Extracellular Matrix / Q000276:immunology / Q000473:pathology; D006801:Humans; D007194:India; D007960:Leukocyte Disorders / Q000209:etiology*; D007963:Leukocytes, Mononuclear / Q000276:immunology* / Q000378:metabolism / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009569:Nitric Oxide / Q000378:metabolism; D011236:Prediabetic State / Q000097:blood / Q000276:immunology / Q000378:metabolism / Q000503:physiopathology*; D019718:Receptors, CXCR4 / Q000235:genetics / Q000378:metabolism
chemical_list: D018821:CD18 Antigens; C527276:CXCR4 protein, human; D019718:Receptors, CXCR4; D009569:Nitric Oxide; C477631:ARG2 protein, human; D001119:Arginase
keywords: Asian Indian; Impaired glucose tolerance; peripheral blood-derived mononuclear cells