title: Ratiometric detection of adenosine triphosphate (ATP) in water and real-time monitoring of apyrase activity with a tripodal zinc complex.
abstract: Two tripodal fluorescent probes Zn⋅L(1,2) have been synthesised, and their anion-binding capabilities were examined by using fluorescence spectroscopy. Probe Zn⋅L(1) allows the selective and ratiometric detection of adenosine triphosphate (ATP) at physiological pH, even in the presence of several competing anions, such as ADP, phosphate and bicarbonate. The probe was applied to the real-time monitoring of the apyrase-catalysed hydrolysis of ATP, in a medium that mimics an extracellular fluid.
authors.affiliation: Department of Chemistry, Durham University, South Road, Durham, DH1 3LE (UK). s.j.butler@dur.ac.uk.
authors.forename: Stephen J
authors.initials: SJ
authors.lastname: Butler
mesh_terms: D000255:Adenosine Triphosphate / Q000032:analysis* / Q000737:chemistry*; D000838:Anions / Q000737:chemistry*; D001081:Apyrase / Q000737:chemistry*; D002384:Catalysis; D056831:Coordination Complexes / Q000138:chemical synthesis* / Q000737:chemistry; D005456:Fluorescent Dyes / Q000138:chemical synthesis* / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008958:Models, Molecular; D011804:Quinolines / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D014867:Water; D015032:Zinc / Q000737:chemistry*
chemical_list: D000838:Anions; D056831:Coordination Complexes; D005456:Fluorescent Dyes; D011804:Quinolines; D014867:Water; D000255:Adenosine Triphosphate; D001081:Apyrase; D015032:Zinc
keywords: ATP; anions; enzymes; fluorescent probes; quinolines