title: Investigation of interaction studies of cefpirome with ACE-inhibitors in various buffers.
abstract: This work describes a RP-HPLC method for the determination and interaction studies of cefpirome with ACE-inhibitors (captopril, enalapril and lisinopril) in various buffers. The separation and interaction of cefpirome with ACE-inhibitors was achieved on a Purospher Star, C18 (5 μm, 250×4.6 mm) column. Mobile phase consisted of methanol: water (80:20, v/v, pH 3.3); however, for the separation of lisinopril, it was modified to methanol-water (40:60, v/v, pH 3.3) and pumped at a flow rate of 1 mL min(-1). In all cases, UV detection was performed at 225 nm. Interactions were carried out in physiological pH i.e., pH 1 (simulated gastric juice), 4 (simulated full stomach), 7.4 (blood pH) and 9 (simulated GI), drug contents were analyzed by reverse phase high performance liquid chromatography. Method was found linear in the concentration range of 1.0-50.0 μg mL(-1) with correlation coefficient (r(2)) of 0.999. Precision (RSD%) was less than 2.0%, indicating good precision of the method and accuracy was 98.0-100.0%. Furthermore, cefpirome-ACE-inhibitors' complexes were also synthesized and results were elucidated on the basis of FT-IR, and (1)H NMR. The interaction results show that these interactions are pH dependent and for the co-administration of cefpirome and ACE-inhibitors, a proper interval should be given.
authors.affiliation: Department of Chemistry, United Arab Emirates University, Al-Ain 15551, United Arab Emirates. Electronic address: nawwaz@gmail.com.
authors.forename: Muhammad
authors.initials: M
authors.lastname: Nawaz
authors.affiliation: Department of Chemistry, University of Karachi, Karachi 75270, Pakistan.
authors.forename: Muhammad Saeed
authors.initials: MS
authors.lastname: Arayne
authors.affiliation: Research Institute of Pharmaceutical Sciences, Faculty of Pharmacy, University of Karachi, Karachi 75270, Pakistan.
authors.forename: Najma
authors.initials: N
authors.lastname: Sultana
authors.affiliation: Department of Pharmacy, University of Nottingham, Kuala Lumpur, Malaysia.
authors.forename: Hira Fatima
authors.initials: HF
authors.lastname: Abbas
mesh_terms: D000806:Angiotensin-Converting Enzyme Inhibitors / Q000737:chemistry*; D002021:Buffers; D002511:Cephalosporins / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D056148:Chromatography, Reverse-Phase / Q000379:methods; D006863:Hydrogen-Ion Concentration; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods; D000097572:Cefpirome
chemical_list: D000806:Angiotensin-Converting Enzyme Inhibitors; D002021:Buffers; D002511:Cephalosporins; D000097572:Cefpirome
keywords: Captopril; Cefpirome; Drug–drug interactions; Enalapril; Lisinopril; RP-HPLC