title: Bioluminescence resonance energy transfer (BRET) to detect the interactions between kappa opioid receptor and non visual arrestins.
abstract: Bioluminescence resonance energy transfer (BRET) is a very sensitive technique employed to study protein-protein interactions, including G-protein-coupled receptors (GPCRs) hetero- and homo-dimerization. Recently, BRET has also been used to investigate the interaction between GPCRs (e.g., β2 adrenergic receptor, muscarinic M2 receptor, dopaminergic D2 receptor) and non-visual arrestins. Here a BRET protocol is described to investigate interactions between the kappa opioid receptor (KOR) and non visual arrestins (arrestin-2 and arrestin-3) in HEK-293 cells, both under basal conditions and after exposure to KOR ligands.
authors.affiliation: Department of Pharmacy and Biotechnology (FaBiT), University of Bologna, Irnerio, 48, Bologna, 40126, Italy, andrea.bedini@unibo.it.
authors.forename: Andrea
authors.initials: A
authors.lastname: Bedini
mesh_terms: D019390:Arrestins / Q000737:chemistry / Q000378:metabolism*; D060807:Bioluminescence Resonance Energy Transfer Techniques; D057809:HEK293 Cells; D006801:Humans; D008967:Molecular Biology / Q000379:methods; D011485:Protein Binding; D060066:Protein Interaction Maps / Q000235:genetics*; D043562:Receptors, G-Protein-Coupled / Q000737:chemistry / Q000378:metabolism; D017473:Receptors, Opioid, kappa / Q000737:chemistry / Q000378:metabolism*
chemical_list: D019390:Arrestins; D043562:Receptors, G-Protein-Coupled; D017473:Receptors, Opioid, kappa