title: Reduced expression of SOX7 in ovarian cancer: a novel tumor suppressor through the Wnt/β-catenin signaling pathway.
abstract: BACKGROUND
  Products of the SOX gene family play important roles in the life process. One of the members, SOX7, is associated with the development of a variety of cancers as a tumor suppression factor, but its relevance with ovarian cancer was unclear. In this study, we investigated the involvement of SOX7 in the progression and prognosis of epithelial ovarian cancer (EOC) and the involved mechanisms.
  
  
  METHODS
  Expression profiles in two independent microarray data sets were analyzed for SOX7 between malignant and normal tissues. The expression levels of SOX7 in EOC, borderline ovarian tumors and normal ovarian tissues were measured by immunohistochemistry. We also measured levels of COX2 and cyclin-D1 to examine their possible involvement in the same signal transduction pathway as SOX7.
  
  
  RESULTS
  The expression of SOX7 was significantly reduced in ovarian cancer tissues compared with normal controls, strongly indicating that SOX7 might be a negative regulator in the Wnt/β-catenin pathway in ovarian cancer. By immunohistochemistry staining, the protein expression of SOX7 showed a consistent trend with that of the gene expression microarray analysis. By contrast, the protein expression level of COX2 and cyclin-D1 increased as the tumor malignancy progressed, suggesting that SOX7 may function through the Wnt/β-catenin signaling pathway as a tumor suppressor. In comparison between the protein expression levels of SOX7 with pathological features of the cancer, we found that SOX7 was down-regulated mainly in serous cystadenocarcinoma and advanced stages of the cancers.
  
  
  CONCLUSIONS
  The expression of SOX7 correlates with tumor progression as a tumor suppressor, possibly through the Wnt/β-catenin signaling pathway in ovarian cancers, suggesting that SOX7 may be a promising prognostic marker.
authors.forename: Huidi
authors.initials: H
authors.lastname: Liu
authors.forename: Zi-Qiao
authors.initials: ZQ
authors.lastname: Yan
authors.forename: Bailiang
authors.initials: B
authors.lastname: Li
authors.forename: Si-Yuan
authors.initials: SY
authors.lastname: Yin
authors.forename: Qiang
authors.initials: Q
authors.lastname: Sun
authors.forename: Jun-Jie
authors.initials: JJ
authors.lastname: Kou
authors.forename: Dan
authors.initials: D
authors.lastname: Ye
authors.forename: Kelsey
authors.initials: K
authors.lastname: Ferns
authors.forename: Hong-Yu
authors.initials: HY
authors.lastname: Liu
authors.forename: Shu-Lin
authors.initials: SL
authors.lastname: Liu
mesh_terms: D000328:Adult; D000368:Aged; D045744:Cell Line, Tumor; D019938:Cyclin D1 / Q000235:genetics / Q000378:metabolism; D051546:Cyclooxygenase 2 / Q000235:genetics / Q000378:metabolism; D066264:Datasets as Topic; D018450:Disease Progression; D015536:Down-Regulation; D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D053263:Gene Regulatory Networks; D006801:Humans; D007150:Immunohistochemistry; D008875:Middle Aged; D060787:Neoplasm Grading; D009367:Neoplasm Staging; D010051:Ovarian Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D055760:SOXF Transcription Factors / Q000235:genetics* / Q000378:metabolism; D060449:Wnt Signaling Pathway; D055815:Young Adult
chemical_list: C484456:SOX7 protein, human; D055760:SOXF Transcription Factors; D019938:Cyclin D1; D051546:Cyclooxygenase 2