title: Biodegradation of aliphatic hydrocarbons in the presence of hydroxy cucurbit[6]uril.
abstract: Aliphatic hydrocarbons are one of the major environmental pollutants with reduced bioavailability. The present study focuses on the effect of hydroxy cucurbit[6]uril on the bioavailability of hydrocarbons. A bacterial consortium was used for biodegradation studies under saline and non-saline conditions. Based on denaturing gradient gel electrophoresis results it was found that the consortium under saline conditions had two different strains. The experiment was conducted in microcosms with tetradecane, hexadecane, octadecane and mixture of the mentioned hydrocarbons as the sole carbon source. The residual hydrocarbon was quantified using gas chromatography every 24h. It was found that biodegradation of tetradecane and hexadecane, as individual carbon source increased in the presence of hydroxy CB[6], probably due to the increase in their bioavailability. In case of octadecane this did not happen. Bioavailability of all three aliphatic hydrocarbons was increased when provided as a mixture to the consortium under saline conditions.
authors.affiliation: Applied and Environmental Biotechnology Laboratory, Department of Biological Sciences, BITS - Pilani, K K Birla Goa Campus, Zuari Nagar, Goa 403 726, India. Electronic address: rajesh.pasumarthi9@gmail.com.
authors.forename: Rajesh
authors.initials: R
authors.lastname: Pasumarthi
authors.affiliation: Department of Chemistry, BITS - Pilani, K K Birla Goa Campus, India. Electronic address: kumarniper@gmail.com.
authors.forename: Vikash
authors.initials: V
authors.lastname: Kumar
authors.affiliation: Applied and Environmental Biotechnology Laboratory, Department of Biological Sciences, BITS - Pilani, K K Birla Goa Campus, Zuari Nagar, Goa 403 726, India. Electronic address: pcsivaraman@gmail.com.
authors.forename: Sivaraman
authors.initials: S
authors.lastname: Chandrasekharan
authors.affiliation: Department of Biological Sciences, BITS - Pilani, K K Birla Goa Campus, India.
authors.forename: Anasuya
authors.initials: A
authors.lastname: Ganguly
authors.affiliation: Department of Chemistry, BITS - Pilani, K K Birla Goa Campus, India. Electronic address: mainak@goa.bits-pilani.ac.in.
authors.forename: Mainak
authors.initials: M
authors.lastname: Banerjee
authors.affiliation: Applied and Environmental Biotechnology Laboratory, Department of Biological Sciences, BITS - Pilani, K K Birla Goa Campus, Zuari Nagar, Goa 403 726, India. Electronic address: srikanth@goa.bits-pilani.ac.in.
authors.forename: Srikanth
authors.initials: S
authors.lastname: Mutnuri
mesh_terms: D000473:Alkanes / Q000378:metabolism / Q000493:pharmacokinetics; D001483:Base Sequence; D001673:Biodegradation, Environmental; D001682:Biological Availability; D001952:Bridged-Ring Compounds / Q000138:chemical synthesis / Q000378:metabolism* / Q000494:pharmacology; D002849:Chromatography, Gas; D058645:Denaturing Gradient Gel Electrophoresis; D006367:HeLa Cells / Q000187:drug effects; D006801:Humans; D006838:Hydrocarbons / Q000378:metabolism* / Q000493:pharmacokinetics; D007093:Imidazoles / Q000138:chemical synthesis / Q000378:metabolism* / Q000494:pharmacology; D009682:Magnetic Resonance Spectroscopy; D059013:Microbial Consortia / Q000235:genetics; D008969:Molecular Sequence Data; D021241:Spectrometry, Mass, Electrospray Ionization; D047028:Macrocyclic Compounds; D000099246:Cucurbit(n)urils
chemical_list: D000473:Alkanes; D001952:Bridged-Ring Compounds; D006838:Hydrocarbons; D007093:Imidazoles; C024713:n-tetradecane; C454805:cucurbit(6)uril; D047028:Macrocyclic Compounds; D000099246:Cucurbit(n)urils; C007932:n-hexadecane; C022883:octadecane
keywords: Bioavailability; Biodegradation; Halophilic; Hydrocarbons; Hydroxy cucurbit[6]uril; Non halophilic