title: Detection of Bacillus thuringiensis Cry1Ab protein based on surface plasmon resonance immunosensor.
abstract: Two novel surface plasmon resonance immunosensors were fabricated for detection of the Bacillus thuringiensis Cry1Ab protein and to demonstrate their performance in analyzing Cry1Ab protein in crop samples. Sensor 2 was modified by 1,6-hexanedithiol, Au/Ag alloy nanoparticles, 3-mercaptopropionic acid, and protein A (or not [sensor 1]), with Cry1Ab monoclonal antibody. As a result, both of the immunosensors exhibited satisfactory linear responses in the Cry1Ab protein concentration ranges of 10 to 500ngml(-1) and 8 to 1000ngml(-1), and the detection limits were 5.0 and 4.8ngml(-1), respectively. The immunosensors possessed good specificity and acceptable reproducibility. In addition, crop samples could be analyzed after a simple treatment. The transgenic crops could be easily identified from the conventional ones by the two immunosensors.
authors.affiliation: College of Chemistry and Material Science, Shandong Agricultural University, Tai'an, Shandong 271018, People's Republic of China.
authors.forename: Huami
authors.initials: H
authors.lastname: Ming
authors.affiliation: College of Chemistry and Material Science, Shandong Agricultural University, Tai'an, Shandong 271018, People's Republic of China.
authors.forename: Manli
authors.initials: M
authors.lastname: Wang
authors.affiliation: College of Chemistry and Material Science, Shandong Agricultural University, Tai'an, Shandong 271018, People's Republic of China. Electronic address: hzyin@sdau.edu.cn.
authors.forename: Hongzong
authors.initials: H
authors.lastname: Yin
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000032:analysis* / Q000235:genetics / Q000276:immunology; D018556:Crops, Agricultural / Q000737:chemistry / Q000235:genetics; D004731:Endotoxins / Q000032:analysis* / Q000235:genetics / Q000276:immunology; D006460:Hemolysin Proteins / Q000032:analysis* / Q000235:genetics / Q000276:immunology; D007306:Insecticides / Q000032:analysis / Q000276:immunology; D057230:Limit of Detection; D053768:Metal Nanoparticles; D030821:Plants, Genetically Modified; D020349:Surface Plasmon Resonance / Q000295:instrumentation / Q000379:methods*
chemical_list: D000911:Antibodies, Monoclonal; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D004731:Endotoxins; D006460:Hemolysin Proteins; D007306:Insecticides; C030956:insecticidal crystal protein, Bacillus Thuringiensis
keywords: Au/Ag alloy nanoparticles; Bacillus thuringiensis Cry1Ab protein; Protein A; Surface plasmon resonance immunosensor; Transgenic crops