title: A high throughput screening assay for identifying glycation inhibitors on MALDI-TOF target.
abstract: The Maillard reaction plays an important role in the food industry, however, the deleterious effects generated by the advanced glycation end-products (AGEs) have been well recognized. Many efforts have been made to seek new AGE inhibitors, in particular those natural ones without adverse effect. We have developed a rapid, mass spectrometry based, on-plate screening assay for novel AGE inhibitors. The glycation reaction, inhibition feedback as well as the subsequent MALDI mass spectrometric analysis occurred on one single MALDI plate. At 1:10 M ratio of peptide to sugar, as little as 4h incubation time allowed the screening test to be ready for analysis. DSP, inhibition and IC50 were calculated to evaluate selected inhibitors and resulting inhibition efficiencies were consistent with available references. We demonstrated that this method provide a potential high throughput screening assay to analyze and identify the anti-glycation agents.
authors.affiliation: State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang, Jiangxi 330047, China.
authors.forename: Qiuting
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang, Jiangxi 330047, China; College of Life Science, Jiangxi Normal University, Nanchang, Jiangxi 330022, China. Electronic address: tuzc_mail@yahoo.com.cn.
authors.forename: Zongcai
authors.initials: Z
authors.lastname: Tu
authors.affiliation: State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang, Jiangxi 330047, China; Engineering Research Center for Biomass Conversion, Ministry of Education, Nanchang University, Nanchang, Jiangxi 330047, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang, Jiangxi 330047, China.
authors.forename: Liangliang
authors.initials: L
authors.lastname: Fan
authors.affiliation: College of Life Science, Jiangxi Normal University, Nanchang, Jiangxi 330022, China.
authors.forename: Xiaoqin
authors.initials: X
authors.lastname: Huang
authors.affiliation: Department of Pathology, Albert Einstein College of Medicine, Yeshiva University, Bronx, NY 10461, United States. Electronic address: hui.xiao@einstein.yu.edu.
authors.forename: Hui
authors.initials: H
authors.lastname: Xiao
mesh_terms: D017127:Glycation End Products, Advanced / Q000737:chemistry*; D006031:Glycosylation; D057166:High-Throughput Screening Assays / Q000379:methods*; D015416:Maillard Reaction; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods
chemical_list: D017127:Glycation End Products, Advanced
keywords: Glycation; Inhibitor; MALDI-TOF; Reaction on target; Screening assay