title: An Acidic PATHOGENESIS-RELATED1 Gene of Oryza grandiglumis is Involved in Disease Resistance Response Against Bacterial Infection.
abstract: Wild rice, Oryza grandiglumis shows hyper-resistance response to pathogen infection. In order to identify genes necessary for defense response in plants, we have carried out a subtractive hybridization coupled with a cDNA macroarray. An acidic PATHOGENESIS-RELATED1 (PR1) gene of the wild rice is highly identical to the acidic PR1 genes of different plant species. The OgPR1a cDNA has an apparent single open reading frame with a predicted molecular mass 40,621 Da and an isoelectic point of 5.14. Both in silico analysis and a transient expression assay in onion epidermal cells revealed that the OgPR1a protein could be localized in intercellular space in plants. The OgPR1a mRNA was strongly transcribed by the exogenous treatment with ethylene and jasmonic acid as well as protein phosphatase inhibitors. Additionally, ectopic expression of the OgPR1a conferred disease resistance on Arabidopsis to the bacterial and fungal infections.
authors.affiliation: National Crop Experiment Station, Rural Development Administration, Suwon 441-100, Korea.
authors.forename: Sang Hyun
authors.initials: SH
authors.lastname: Shin
authors.affiliation: Department of Genetic Engineering, Dong-A University, Busan 604-714, Korea.
authors.forename: Jung-Hun
authors.initials: JH
authors.lastname: Pak
authors.affiliation: Department of Genetic Engineering, Dong-A University, Busan 604-714, Korea.
authors.forename: Mi Jin
authors.initials: MJ
authors.lastname: Kim
authors.affiliation: Department of Genetic Engineering, Dong-A University, Busan 604-714, Korea.
authors.forename: Hye Jeong
authors.initials: HJ
authors.lastname: Kim
authors.affiliation: Department of Genetic Engineering, Dong-A University, Busan 604-714, Korea.
authors.forename: Ju Sung
authors.initials: JS
authors.lastname: Oh
authors.affiliation: Department of Genetic Engineering, Dong-A University, Busan 604-714, Korea.
authors.forename: Hong Kyu
authors.initials: HK
authors.lastname: Choi
authors.affiliation: Department of Genetic Engineering, Dong-A University, Busan 604-714, Korea.
authors.forename: Ho Won
authors.initials: HW
authors.lastname: Jung
authors.affiliation: Department of Genetic Engineering, Dong-A University, Busan 604-714, Korea.
authors.forename: Young Soo
authors.initials: YS
authors.lastname: Chung
keywords: Arabidopsis thaliana; Oryza grandiglumis; Pseudomonas syringae; acidic PATHOGENESIS-RELATED1 gene; disease resistance