title: First case of Zika virus infection in a returning Canadian traveler.
abstract: A woman who recently traveled to Thailand came to a local emergency department with a fever and papular rash. She was tested for measles, malaria, and dengue. Positive finding for IgM antibody against dengue and a failure to seroconvert for IgG against dengue for multiple blood samples suggested an alternate flavivirus etiology. Amplification of a conserved region of the non-structural protein 5 gene of the genus Flavivirus yielded a polymerase chain reaction product with a matching sequence of 99% identity with Zika virus. A urine sample and a nasopharygeal swab specimen obtained for the measles investigation were also positive for this virus by reverse transcription polymerase chain reaction. Subsequently, the urine sample yielded a Zika virus isolate in cell culture. This case report describes a number of novel clinical and laboratory findings, the first documentation of this virus in Canada, and the second documentation from this region in Thailand.
authors.affiliation: Provincial Laboratory for Public Health, Calgary, Alberta, Canada; Alberta Health Services, Calgary, Alberta, Canada; National Microbiology Laboratory; Public Health Agency of Canada, Winnipeg, Manitoba, Canada; University of Calgary, Calgary, Alberta, Canada; University of Manitoba, Winnipeg, Manitoba, Canada kevin.fonseca@albertahealthservices.ca.
authors.forename: Kevin
authors.initials: K
authors.lastname: Fonseca
authors.affiliation: Provincial Laboratory for Public Health, Calgary, Alberta, Canada; Alberta Health Services, Calgary, Alberta, Canada; National Microbiology Laboratory; Public Health Agency of Canada, Winnipeg, Manitoba, Canada; University of Calgary, Calgary, Alberta, Canada; University of Manitoba, Winnipeg, Manitoba, Canada.
authors.forename: Bonnie
authors.initials: B
authors.lastname: Meatherall
authors.affiliation: Provincial Laboratory for Public Health, Calgary, Alberta, Canada; Alberta Health Services, Calgary, Alberta, Canada; National Microbiology Laboratory; Public Health Agency of Canada, Winnipeg, Manitoba, Canada; University of Calgary, Calgary, Alberta, Canada; University of Manitoba, Winnipeg, Manitoba, Canada.
authors.forename: Danielle
authors.initials: D
authors.lastname: Zarra
authors.affiliation: Provincial Laboratory for Public Health, Calgary, Alberta, Canada; Alberta Health Services, Calgary, Alberta, Canada; National Microbiology Laboratory; Public Health Agency of Canada, Winnipeg, Manitoba, Canada; University of Calgary, Calgary, Alberta, Canada; University of Manitoba, Winnipeg, Manitoba, Canada.
authors.forename: Michael
authors.initials: M
authors.lastname: Drebot
authors.affiliation: Provincial Laboratory for Public Health, Calgary, Alberta, Canada; Alberta Health Services, Calgary, Alberta, Canada; National Microbiology Laboratory; Public Health Agency of Canada, Winnipeg, Manitoba, Canada; University of Calgary, Calgary, Alberta, Canada; University of Manitoba, Winnipeg, Manitoba, Canada.
authors.forename: Judy
authors.initials: J
authors.lastname: MacDonald
authors.affiliation: Provincial Laboratory for Public Health, Calgary, Alberta, Canada; Alberta Health Services, Calgary, Alberta, Canada; National Microbiology Laboratory; Public Health Agency of Canada, Winnipeg, Manitoba, Canada; University of Calgary, Calgary, Alberta, Canada; University of Manitoba, Winnipeg, Manitoba, Canada.
authors.forename: Kanti
authors.initials: K
authors.lastname: Pabbaraju
authors.affiliation: Provincial Laboratory for Public Health, Calgary, Alberta, Canada; Alberta Health Services, Calgary, Alberta, Canada; National Microbiology Laboratory; Public Health Agency of Canada, Winnipeg, Manitoba, Canada; University of Calgary, Calgary, Alberta, Canada; University of Manitoba, Winnipeg, Manitoba, Canada.
authors.forename: Sallene
authors.initials: S
authors.lastname: Wong
authors.affiliation: Provincial Laboratory for Public Health, Calgary, Alberta, Canada; Alberta Health Services, Calgary, Alberta, Canada; National Microbiology Laboratory; Public Health Agency of Canada, Winnipeg, Manitoba, Canada; University of Calgary, Calgary, Alberta, Canada; University of Manitoba, Winnipeg, Manitoba, Canada.
authors.forename: Patricia
authors.initials: P
authors.lastname: Webster
authors.affiliation: Provincial Laboratory for Public Health, Calgary, Alberta, Canada; Alberta Health Services, Calgary, Alberta, Canada; National Microbiology Laboratory; Public Health Agency of Canada, Winnipeg, Manitoba, Canada; University of Calgary, Calgary, Alberta, Canada; University of Manitoba, Winnipeg, Manitoba, Canada.
authors.forename: Robbin
authors.initials: R
authors.lastname: Lindsay
authors.affiliation: Provincial Laboratory for Public Health, Calgary, Alberta, Canada; Alberta Health Services, Calgary, Alberta, Canada; National Microbiology Laboratory; Public Health Agency of Canada, Winnipeg, Manitoba, Canada; University of Calgary, Calgary, Alberta, Canada; University of Manitoba, Winnipeg, Manitoba, Canada.
authors.forename: Raymond
authors.initials: R
authors.lastname: Tellier
mesh_terms: D000914:Antibodies, Viral / Q000097:blood; D002170:Canada; D003715:Dengue / Q000097:blood / Q000175:diagnosis / Q000276:immunology; D003951:Diagnostic Errors; D005260:Female; D005334:Fever; D006801:Humans; D007075:Immunoglobulin M / Q000097:blood; D016133:Polymerase Chain Reaction; D012367:RNA, Viral / Q000302:isolation & purification; D013785:Thailand; D014195:Travel; D000071244:Zika Virus / Q000302:isolation & purification*; D000071243:Zika Virus Infection / Q000097:blood / Q000175:diagnosis* / Q000453:epidemiology* / Q000276:immunology
chemical_list: D000914:Antibodies, Viral; D007075:Immunoglobulin M; D012367:RNA, Viral