title: Immunohistochemical identification of osteoclasts and multinucleated macrophages.
abstract: Osteoclasts are bone-resorbing multinuclear cells derived from hematopoietic stem cells which are specialised to carry out lacunar bone resorption. The immunophenotype of giant cell-containing bone lesions in a wide range of osteoclast-like giant cells was similarly assessed. Both multinucleated macrophages and osteoclasts were found to express CD68. Multinucleated macrophages, but not osteoclasts, expressed GrB and Ki67. CD13+/CD14+/CD68+/GrB-/Ki67-/CD56- all giant-cell lesions noted in giant cells of bone. Giant cells have an osteoclast phenotype in most giant cell-rich lesions of bone, which do not express the macrophage-associated antigens GrB and Ki67. Our results indicate that they are formed from osteoclast precursors of mononuclear phagocyte.
authors.affiliation: Department of Endocrinology, Key Laboratory of Endocrinology, Fujian Provincial Hospital, Fujian Medical University, Fuzhou 350001, China.
authors.forename: Jian-Ming
authors.initials: JM
authors.lastname: Hou
authors.affiliation: Department of Endocrinology, Key Laboratory of Endocrinology, Fujian Provincial Hospital, Fujian Medical University, Fuzhou 350001, China. Electronic address: linjianli@hotmail.com.
authors.forename: Jian-Li
authors.initials: JL
authors.lastname: Lin
authors.affiliation: Department of Endocrinology, Key Laboratory of Endocrinology, Fujian Provincial Hospital, Fujian Medical University, Fuzhou 350001, China.
authors.forename: Jun-Ping
authors.initials: JP
authors.lastname: Wen
authors.affiliation: Department of Pathology, Fujian Provincial Hospital, Fuzhou 350001, China.
authors.forename: Long
authors.initials: L
authors.lastname: Jin
authors.affiliation: Department of Orthopedics, Fujian Provincial Hospital, Fuzhou 350001, China.
authors.forename: Fa-Qiang
authors.initials: FQ
authors.lastname: Tang
mesh_terms: D000941:Antigens / Q000276:immunology; D001842:Bone and Bones / Q000276:immunology; D015726:Giant Cells / Q000276:immunology; D006801:Humans; D007150:Immunohistochemistry; D016130:Immunophenotyping; D008264:Macrophages / Q000276:immunology*; D010010:Osteoclasts / Q000276:immunology*; D010587:Phagocytosis
chemical_list: D000941:Antigens
keywords: Immunophenotype; MPS; Multinucleated macrophages; Osteoclasts; RANKL