title: Sasa quelpaertensis leaf extract suppresses dextran sulfate sodium-induced colitis in mice by inhibiting the proinflammatory mediators and mitogen-activated protein kinase phosphorylation.
abstract: Sasa quelpaertensis leaves exert anti-inflammatory and anticarcinogenic effects, although it remains unclear whether these leaves can suppress inflammation-related intestinal diseases. This study hypothesized that Sasa quelpaertensis leaf extract (SQE) exerts a protective effect against inflammation in a dextran sulfate sodium (DSS)-induced colitis mouse model. Therefore, colon tissues of DSS-induced colitis mice that were treated with SQE were assayed for levels of proinflammatory markers, mitogen-activated protein kinase signaling, and activation of nuclear factor κB. For this purpose, mice were pretreated with SQE (100 mg/kg or 300 mg/kg body weight) by gavage for a 2-week period. Mice then received either SQE or sulfasalazine (100 mg/kg body weight) with 2.5% DSS in drinking water for 7 days twice daily and 7 days of tap water ad libitum between DSS treatment. Treatment with SQE was found to attenuate the severity of DSS-induced colitis, as assessed by disease activity index scores, shrinkage of colon length, and histopathologic changes. SQE reduced DSS-induced proliferation in distal colon tissues. It also significantly suppressed levels of tumor necrosis factor-α in serum and colon tissues, nitric oxide synthase, cyclooxygenase, and levels of phosphorylated c-Jun N-terminal kinases, p38, extracellular-signal-regulated kinases 1/2, and IκBα in colon tissues. To our knowledge, this is the first study to demonstrate that SQE supplementation can exert an anti-inflammatory effect on experimental chronic colitis.
authors.affiliation: Department of Nutritional Science and Food Management, Ewha Womans University, Seoul 120-750, Republic of Korea.
authors.forename: Kyung-Mi
authors.initials: KM
authors.lastname: Kim
authors.affiliation: Department of Nutritional Science and Food Management, Ewha Womans University, Seoul 120-750, Republic of Korea.
authors.forename: Yoo-Sun
authors.initials: YS
authors.lastname: Kim
authors.affiliation: Department of Nutritional Science and Food Management, Ewha Womans University, Seoul 120-750, Republic of Korea.
authors.forename: Ji Ye
authors.initials: JY
authors.lastname: Lim
authors.affiliation: Department of Nutritional Science and Food Management, Ewha Womans University, Seoul 120-750, Republic of Korea.
authors.forename: Soo Jin
authors.initials: SJ
authors.lastname: Min
authors.affiliation: Department of Biomedical Laboratory Science, Eulji University, Seongnam-si, Kyeonggi-do 461-713, Republic of Korea.
authors.forename: Jae-Ho
authors.initials: JH
authors.lastname: Shin
authors.affiliation: Jeju Sasa Industry Development Agency, Jeju National University, Jejusi, Jeju 690-756, Republic of Korea.
authors.forename: Hee-Chul
authors.initials: HC
authors.lastname: Ko
authors.affiliation: Department of Biology, Jeju National University, Jejusi, Jeju 690-756, Republic of Korea.
authors.forename: Se-Jae
authors.initials: SJ
authors.lastname: Kim
authors.affiliation: Department of Food and Nutrition, Kyung Hee University, Seoul 130-701, Republic of Korea.
authors.forename: Yunsook
authors.initials: Y
authors.lastname: Lim
authors.affiliation: Department of Nutritional Science and Food Management, Ewha Womans University, Seoul 120-750, Republic of Korea. Electronic address: yuri.kim@ewha.ac.kr.
authors.forename: Yuri
authors.initials: Y
authors.lastname: Kim
mesh_terms: D000818:Animals; D003092:Colitis / Q000139:chemically induced / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D003106:Colon / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D051546:Cyclooxygenase 2 / Q000378:metabolism; D016264:Dextran Sulfate; D020672:I-kappa B Proteins / Q000378:metabolism; D018836:Inflammation Mediators / Q000378:metabolism*; D048031:JNK Mitogen-Activated Protein Kinases / Q000378:metabolism; D008297:Male; D008810:Mice, Inbred C57BL; D019950:Mitogen-Activated Protein Kinase 1 / Q000378:metabolism; D048052:Mitogen-Activated Protein Kinase 3 / Q000378:metabolism; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism*; D000072000:NF-KappaB Inhibitor alpha; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism; D010766:Phosphorylation; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use*; D018515:Plant Leaves; D031787:Sasa; D012720:Severity of Illness Index; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D020672:I-kappa B Proteins; D018836:Inflammation Mediators; C000606205:Nfkbia protein, mouse; D010936:Plant Extracts; D014409:Tumor Necrosis Factor-alpha; D000072000:NF-KappaB Inhibitor alpha; D016264:Dextran Sulfate; D052247:Nitric Oxide Synthase Type II; D051546:Cyclooxygenase 2; D048031:JNK Mitogen-Activated Protein Kinases; D019950:Mitogen-Activated Protein Kinase 1; D048052:Mitogen-Activated Protein Kinase 3; D020928:Mitogen-Activated Protein Kinases
keywords: Anti-inflammation; Dextran sulfate sodium; Mice; Sasa quelpaertensis Nakai leaf extract; Ulcerative colitis