title: Trachyspermum ammi (L.) sprague: chemical composition of essential oil and antimicrobial activities of respective fractions.
abstract: Resistance to antibacterial agents has become a serious problem for global health. The current study evaluated the antimicrobial activities of essential oil and respective fractions of Trachyspermum ammi (L.) Sprague. Seeds of the essential oil were extracted and fractionated using column chromatography. All fractions were then analyzed by gas chromatography/mass spectrometry. Antifungal and antibacterial activities of the oil and its fractions were assessed using microdilution method. Compounds γ-terpinene (48.07%), ρ-cymene (33.73%), and thymol (17.41%) were determined as major constituents. The effect of fraction II was better than total essential oil, fraction I, and standard thymol. The greater effect of fraction II compared to standard thymol showed the synergistic effects of the ingredients in this fraction. As this fraction and also total oil were effective on the studied microorganism, the combination of these products with current antimicrobial agents could be considered as new antimicrobial compounds in further investigations.
authors.affiliation: Medicinal Plants Processing Research Center, Shiraz University of Medical Sciences, Shiraz, Iran Department of Pharmacognosy, School of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Mahmoodreza R
authors.initials: MR
authors.lastname: Moein
authors.affiliation: Department of Medical Mycology and Parasitology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Kamiar
authors.initials: K
authors.lastname: Zomorodian
authors.affiliation: Department of Medical Mycology and Parasitology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Keyvan
authors.initials: K
authors.lastname: Pakshir
authors.affiliation: Medicinal Plants Processing Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Farnoosh
authors.initials: F
authors.lastname: Yavari
authors.affiliation: Medicinal Plants Processing Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Marjan
authors.initials: M
authors.lastname: Motamedi
authors.affiliation: Medicinal Plants Processing Research Center, Shiraz University of Medical Sciences, Shiraz, Iran Department of Phytopharmaceuticals (Traditional Pharmacy), School of Pharmacy and Pharmaceutical Sciences Research Center, Shiraz University of Medical Sciences, Shiraz, Iran zarm@sums.ac.ir.
authors.forename: Mohammad M
authors.initials: MM
authors.lastname: Zarshenas
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000494:pharmacology; D000935:Antifungal Agents / Q000737:chemistry* / Q000494:pharmacology; D019661:Apiaceae / Q000737:chemistry*; D001419:Bacteria / Q000187:drug effects; D005658:Fungi / Q000187:drug effects; D009822:Oils, Volatile / Q000737:chemistry* / Q000494:pharmacology; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D010938:Plant Oils / Q000737:chemistry* / Q000494:pharmacology; D012639:Seeds / Q000737:chemistry
chemical_list: D000900:Anti-Bacterial Agents; D000935:Antifungal Agents; D009822:Oils, Volatile; D010936:Plant Extracts; D010938:Plant Oils
keywords: antimicrobial activity; essential oil; nosocomial infection