title: Invivoscribe BIOMED-2 primer mixes in B-cell immunoglobulin gene rearrangement studies: experience of a molecular diagnostics laboratory in a major tertiary care center.
abstract: OBJECTIVE
  To determine the frequency of positive reactions obtained using the Invivoscribe BIOMED-2 kit for B-cell gene rearrangement studies in leukemias and lymphomas.
  
  
  METHODS
  We reviewed the gel patterns for 192 samples tested, using the above-mentioned kit and matched the positive signal with the corresponding mix available in the assay kit.
  
  
  RESULTS
  92.2% had immunoglobulin heavy-chain clonality, of which 74% were detected by the IgH VH-FR1+JH primer set, 75.5% by IgH VH-FR2+JH primer set, 65.1% by IgH VH-FR3+JH primer set, 26% by IgH DH+JH primer set, and 2.1% by IgH DH7+JH primer set. In addition, 55.7% had clonality in the kappa light chain, where 33.3% were positive by the IgK Vκ +Jκ primer set and 39.6% by IgK Vκ and INTR+Kde primer sets. Clonality in the lambda light chain of immunoglobulins was detected in 17.7% of specimens tested using the IgL Vλ +Jλ primer set.
  
  
  CONCLUSIONS
  All primer mixes provided by the assay were positive. Thus, the Invivoscribe BIOMED-2 B-cell gene rearrangement kit is very reliable in adequately covering all targets represented by the master mixes. This assay is an integral part of the differential diagnosis of clonal populations of cells. Our report is the first in the literature that describes the full range of coverage of the BIOMED-2 primer mixes provided in this assay.
authors.affiliation: 1 Department of Pathology and Laboratory Medicine, American University of Beirut Medical Center , Beirut, Lebanon .
authors.forename: Fatmeh
authors.initials: F
authors.lastname: Abbas
authors.forename: Souha N
authors.initials: SN
authors.lastname: Yazbek
authors.forename: Dina
authors.initials: D
authors.lastname: Shammaa
authors.forename: Rouba
authors.initials: R
authors.lastname: Hoteit
authors.forename: Puzant
authors.initials: P
authors.lastname: Fermanian
authors.forename: Rami
authors.initials: R
authors.lastname: Mahfouz
mesh_terms: D017931:DNA Primers / Q000737:chemistry* / Q000235:genetics; D005260:Female; D015326:Gene Rearrangement, B-Lymphocyte, Heavy Chain; D015328:Gene Rearrangement, B-Lymphocyte, Light Chain; D006801:Humans; D015448:Leukemia, B-Cell / Q000235:genetics*; D016393:Lymphoma, B-Cell / Q000235:genetics*; D008297:Male; D057089:Pathology, Molecular / Q000379:methods; D016133:Polymerase Chain Reaction / Q000379:methods*; D062606:Tertiary Care Centers
chemical_list: D017931:DNA Primers