title: Cryopreservation of bull sperm: effects of extender supplemented with different cryoprotectants and antioxidants on sperm motility, antioxidant capacity and fertility results.
abstract: The objectives of this study were to compare glycerol (G) and ethylene glycol (EG) at different concentrations and trehalose (T) or cysteine (C; with/without) in Tris extender for cryopreservation of bull semen. Twenty-four ejaculates obtained from three bulls were included in the study. Each ejaculate was divided into four equal aliquots and diluted using both of the Tris extenders with G (5% or 7%) or EG (3% or 5%). After that, each extenders were divided into three equal aliquots and diluted using both of the 5 mM C or 25 mM T, and control (without additives) was cooled to 4 ° C and frozen in 0.25 ml French straws. The addition of 3% and 5% EG without antioxidants resulted in the least Computer-Assisted Sperm motility Analysis (CASA) motility as compared with the other groups. Treatment with 25 mM T in 3% EG beneficially effected acrosome morphology as compared with the other groups. Also, treatment with 3% EG with 25 mM T and 5% EG resulted in a greater rate of total abnormalities. Treatment with 3% G yielded a slightly greater percentage of membrane integrity by Hypo-Osmotic Swelling Test (HOST) assessment than that of the other groups. Treatment with 3% EG with 5 mM C resulted in the greatest concentration of malondialdehyde (MDA). The glutathione peroxidase (GPx) antioxidant activity was increased in the C-treatment groups when compared to the other groups. Treatment with 5% EG and 5 mM C resulted in less chromatin damage and detrimental impacts on tail moment. Treatment with 5% EG led to greater non-return rates of inseminated cows. However, this result was not considered to be statistically important.
authors.affiliation: Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Aksaray University, TR68100 Aksaray, Turkey.
authors.forename: Serhat
authors.initials: S
authors.lastname: Büyükleblebici
authors.affiliation: Aksaray Technical Sciences Vocational School, Aksaray University, TR68100 Aksaray, Turkey. Electronic address: barbarostuncerp@hotmail.com.
authors.forename: Pürhan Barbaros
authors.initials: PB
authors.lastname: Tuncer
authors.affiliation: Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Selcuk University, Konya, Turkey.
authors.forename: Mustafa Numan
authors.initials: MN
authors.lastname: Bucak
authors.affiliation: Department of Pharmaceutical Toxicology, Faculty of Pharmacy, Erciyes University, 38039 Kayseri, Turkey.
authors.forename: Ayşe
authors.initials: A
authors.lastname: Eken
authors.affiliation: Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Erciyes University, 38039 Kayseri, Turkey.
authors.forename: Serpil
authors.initials: S
authors.lastname: Sarıözkan
authors.affiliation: Aksaray Technical Sciences Vocational School, Aksaray University, TR68100 Aksaray, Turkey.
authors.forename: Umut
authors.initials: U
authors.lastname: Taşdemir
authors.affiliation: Department of Pharmaceutical Toxicology, Faculty of Pharmacy, Erciyes University, 38039 Kayseri, Turkey.
authors.forename: Burcu Ünlü
authors.initials: BÜ
authors.lastname: Endirlik
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002417:Cattle; D015925:Cryopreservation / Q000662:veterinary*; D003451:Cryoprotective Agents / Q000494:pharmacology*; D004789:Enzyme Activation; D005298:Fertility / Q000187:drug effects; D005979:Glutathione Peroxidase / Q000378:metabolism; D005990:Glycerol; D008297:Male; D018384:Oxidative Stress; D012662:Semen Preservation / Q000379:methods / Q000662:veterinary*; D013081:Sperm Motility / Q000187:drug effects; D013094:Spermatozoa / Q000187:drug effects*
chemical_list: D000975:Antioxidants; D003451:Cryoprotective Agents; D005979:Glutathione Peroxidase; D005990:Glycerol
keywords: Antioxidants; Bull semen; Cryoprotectant; Fertility; Freezing