title: Characterization of novel microsatellite markers derived from Korean rose bitterling (Rhodeus uyekii) genomic library.
abstract: Korean rose bitterling (Rhodeus uyekii) is a freshwater fish endemic to Korea. Natural populations of this species have experienced severe declines as a result of habitat fragmentation and water pollution. To conserve and restore R. uyekii, the genetic diversity of this species needs to be assessed at the population level. Eighteen novel polymorphic microsatellite loci for R. uyekii were developed using an enriched partial genomic library. Polymorphisms at these loci were studied in 150 individuals collected from three populations. The number of alleles at each locus ranged from 3 to 47 (mean = 17.1). Within the populations, the observed heterozygosity ranged from 0.032 to 1.000, expected heterozygosity from 0.082 to 0.967, and polymorphism information content from 0.078 to 0.950. Six loci showed significant deviation from Hardy-Weinberg equilibrium after Bonferroni's correction, and no significant linkage disequilibrium was detected between most locus pairs, except in three cases. These highly informative microsatellite markers should be useful for genetic population structure analyses of R. uyekii.
authors.affiliation: Biotechnology Research Division, National Fisheries Research and Development Institute, Busan, Republic of Korea wj2464@korea.kr.
authors.forename: W J
authors.initials: WJ
authors.lastname: Kim
authors.affiliation: Biotechnology Research Division, National Fisheries Research and Development Institute, Busan, Republic of Korea.
authors.forename: E H
authors.initials: EH
authors.lastname: Shin
authors.affiliation: Biotechnology Research Division, National Fisheries Research and Development Institute, Busan, Republic of Korea.
authors.forename: H J
authors.initials: HJ
authors.lastname: Kong
authors.affiliation: Biotechnology Research Division, National Fisheries Research and Development Institute, Busan, Republic of Korea.
authors.forename: H S
authors.initials: HS
authors.lastname: Kim
authors.affiliation: Biotechnology Research Division, National Fisheries Research and Development Institute, Busan, Republic of Korea.
authors.forename: B S
authors.initials: BS
authors.lastname: Kim
authors.affiliation: Biotechnology Research Division, National Fisheries Research and Development Institute, Busan, Republic of Korea.
authors.forename: B H
authors.initials: BH
authors.lastname: Nam
authors.affiliation: Biotechnology Research Division, National Fisheries Research and Development Institute, Busan, Republic of Korea.
authors.forename: Y O
authors.initials: YO
authors.lastname: Kim
authors.affiliation: Central Regional Inland Fisheries Research, National Fisheries Research and Development Institute, Gyeonggi-do, Republic of Korea.
authors.forename: C H
authors.initials: CH
authors.lastname: Kim
authors.affiliation: Biotechnology Research Division, National Fisheries Research and Development Institute, Busan, Republic of Korea.
authors.forename: H
authors.initials: H
authors.lastname: Jung
authors.affiliation: Biotechnology Research Division, National Fisheries Research and Development Institute, Busan, Republic of Korea.
authors.forename: C M
authors.initials: CM
authors.lastname: An
mesh_terms: D000483:Alleles; D000818:Animals; D005399:Fishes / Q000235:genetics*; D015698:Genomic Library; D005838:Genotype; D018895:Microsatellite Repeats; D011110:Polymorphism, Genetic