title: Surface modifications of dental ceramic implants with different glass solder matrices: in vitro analyses with human primary osteoblasts and epithelial cells.
abstract: Ceramic materials show excellent esthetic behavior, along with an absence of hypersensitivity, making them a possible alternative implant material in dental surgery. However, their surface properties enable only limited osseointegration compared to titanium implants. Within this study, a novel surface coating technique for enhanced osseointegration was investigated biologically and mechanically. Specimens of tetragonal zirconia polycrystal (TZP) and aluminum toughened zirconia (ATZ) were modified with glass solder matrices in two configurations which mainly consisted of SiO2, Al2O3, K2O, and Na2O. The influence on human osteoblastic and epithelial cell viability was examined by means of a WST-1 assay as well as live/dead staining. A C1CP-ELISA was carried out to verify procollagen type I production. Uncoated/sandblasted ceramic specimens and sandblasted titanium surfaces were investigated as a reference. Furthermore, mechanical investigations of bilaterally coated pellets were conducted with respect to surface roughness and adhesive strength of the different coatings. These tests could demonstrate a mechanically stable implant coating with glass solder matrices. The coated ceramic specimens show enhanced osteoblastic and partly epithelial viability and matrix production compared to the titanium control. Hence, the new glass solder matrix coating could improve bone cell growth as a prerequisite for enhanced osseointegration of ceramic implants.
authors.affiliation: Department of Orthopaedics, Research Lab for Biomechanics and Implant Technology, University Medicine Rostock, Doberaner Straße 142, 18057 Rostock, Germany.
authors.forename: Jana
authors.identifier: 0000-0002-0250-9121
authors.initials: J
authors.lastname: Markhoff
authors.affiliation: Department of Orthopaedics, Research Lab for Biomechanics and Implant Technology, University Medicine Rostock, Doberaner Straße 142, 18057 Rostock, Germany.
authors.forename: Enrico
authors.initials: E
authors.lastname: Mick
authors.affiliation: ZM Praezisionsdentaltechnik GmbH, Breite Straße 16, 18055 Rostock, Germany.
authors.forename: Aurica
authors.identifier: 0000-0002-8187-6068
authors.initials: A
authors.lastname: Mitrovic
authors.affiliation: Department of Orthopaedics, Research Lab for Biomechanics and Implant Technology, University Medicine Rostock, Doberaner Straße 142, 18057 Rostock, Germany.
authors.forename: Juliane
authors.identifier: 0000-0002-6763-4361
authors.initials: J
authors.lastname: Pasold
authors.affiliation: Department of Orthopaedics, Research Lab for Biomechanics and Implant Technology, University Medicine Rostock, Doberaner Straße 142, 18057 Rostock, Germany.
authors.forename: Katharina
authors.identifier: 0000-0003-2423-201X
authors.initials: K
authors.lastname: Wegner
authors.affiliation: Department of Orthopaedics, Research Lab for Biomechanics and Implant Technology, University Medicine Rostock, Doberaner Straße 142, 18057 Rostock, Germany.
authors.forename: Rainer
authors.identifier: 0000-0002-9157-1858
authors.initials: R
authors.lastname: Bader
mesh_terms: D002516:Ceramics / Q000009:adverse effects; D020099:Coated Materials, Biocompatible / Q000009:adverse effects; D015921:Dental Implants / Q000009:adverse effects*; D003764:Dental Materials / Q000009:adverse effects; D004847:Epithelial Cells / Q000187:drug effects*; D005898:Glass / Q000737:chemistry; D006801:Humans; D016348:Osseointegration / Q000187:drug effects*; D010006:Osteoblasts / Q000187:drug effects*; D061251:Primary Cell Culture; D012822:Silicon Dioxide / Q000378:metabolism; D013499:Surface Properties; D014025:Titanium / Q000009:adverse effects
chemical_list: D020099:Coated Materials, Biocompatible; D015921:Dental Implants; D003764:Dental Materials; D012822:Silicon Dioxide; D014025:Titanium