title: The role of nitric oxide synthase signaling pathway in the Zn-induced cellular responses in MCF-7 cells.
abstract: Trace amount zinc plays key roles in biological systems, while in excessive amount it causes toxic effects. Evidence shows that there exists a crosstalk between NO and Zn apoptotic signal transduction pathway. However, the potential mechanism of Zn-induced cellular responses through the NOS signaling pathway has not been determined yet. In this research, trace amount ZnCl2 (1nM) could induce the NO production, however it appears that this influence does not extend to genetic level in MCF-7 cells. Whereas, excess ZnCl2 (100μM, 1mM) could lead to a decreased NO production first with the release of Ca(2+), and then induce the NO production with the transcriptional and translational activation of NOSs. The ROS generation was also induced by excess ZnCl2, causing the elF2α phosphorylation. The alleviation effect of N-acetyl-l-cysteine or l-arginine on the Zn-induced ROS generation and apoptosis suggested that Zn-induced apoptosis was associated with the NOS-mediated oxidative stress.
authors.affiliation: School of Environmental Science and Engineering, Shanghai Jiao Tong University, Shanghai 200240, PR China; School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, PR China; Bor S. Luh Food Safety Research Center, Shanghai 200240, PR China.
authors.forename: Lingying
authors.initials: L
authors.lastname: Zhong
authors.affiliation: School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, PR China; Key Laboratory of Urban Agriculture (South) of Ministry of Agriculture, Shanghai 200240, PR China; Bor S. Luh Food Safety Research Center, Shanghai 200240, PR China.
authors.forename: Lumei
authors.initials: L
authors.lastname: Wang
authors.affiliation: School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, PR China; Key Laboratory of Urban Agriculture (South) of Ministry of Agriculture, Shanghai 200240, PR China; Bor S. Luh Food Safety Research Center, Shanghai 200240, PR China.
authors.forename: Lurong
authors.initials: L
authors.lastname: Xu
authors.affiliation: School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, PR China; Key Laboratory of Urban Agriculture (South) of Ministry of Agriculture, Shanghai 200240, PR China; Bor S. Luh Food Safety Research Center, Shanghai 200240, PR China.
authors.forename: Qunlu
authors.initials: Q
authors.lastname: Liu
authors.affiliation: School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, PR China; Key Laboratory of Urban Agriculture (South) of Ministry of Agriculture, Shanghai 200240, PR China; Bor S. Luh Food Safety Research Center, Shanghai 200240, PR China; Shanghai Food Safety Engineering Research Center, Shanghai 200240, PR China.
authors.forename: Linlei
authors.initials: L
authors.lastname: Jiang
authors.affiliation: School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, PR China; Key Laboratory of Urban Agriculture (South) of Ministry of Agriculture, Shanghai 200240, PR China; Bor S. Luh Food Safety Research Center, Shanghai 200240, PR China.
authors.forename: Yuee
authors.initials: Y
authors.lastname: Zhi
authors.affiliation: School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, PR China; Key Laboratory of Urban Agriculture (South) of Ministry of Agriculture, Shanghai 200240, PR China; Bor S. Luh Food Safety Research Center, Shanghai 200240, PR China. Electronic address: weilu@sjtu.edu.cn.
authors.forename: Wei
authors.initials: W
authors.lastname: Lu
authors.affiliation: School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, PR China; Key Laboratory of Urban Agriculture (South) of Ministry of Agriculture, Shanghai 200240, PR China; Bor S. Luh Food Safety Research Center, Shanghai 200240, PR China. Electronic address: peizhousjtu@163.com.
authors.forename: Pei
authors.initials: P
authors.lastname: Zhou
mesh_terms: D000111:Acetylcysteine / Q000494:pharmacology; D017209:Apoptosis; D001120:Arginine / Q000031:analogs & derivatives / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism*; D049109:Cell Proliferation / Q000187:drug effects; D002712:Chlorides / Q000494:pharmacology*; D005260:Female; D006801:Humans; D061986:MCF-7 Cells; D009569:Nitric Oxide / Q000378:metabolism*; D019001:Nitric Oxide Synthase / Q000235:genetics* / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D010766:Phosphorylation; D017382:Reactive Oxygen Species / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects*; D014157:Transcription Factors / Q000378:metabolism; D017967:Zinc Compounds / Q000494:pharmacology*
chemical_list: D002712:Chlorides; D017382:Reactive Oxygen Species; D014157:Transcription Factors; D017967:Zinc Compounds; C101003:ELF2 protein, human; D009569:Nitric Oxide; C016837:zinc chloride; D001120:Arginine; D019001:Nitric Oxide Synthase; D002118:Calcium; C000015:N-acetyl-L-arginine; D000111:Acetylcysteine
keywords: Apoptosis; Heavy metal; Nitric oxide synthase (NOS); Reactive oxygen species (ROS); The alpha subunit of eukaryotic initiation factor 2 (elF2α) phosphorylation; Zinc (Zn)