title: In vitro apoptotic effect of cassaine-type diterpene amides from Erythrophleum fordii on PC-3 prostate cancer cells.
abstract: Cytotoxic activity-guided fractionation of Erythrophleum fordii led to the isolation of two new cassaine diterpenoid-diterpenoid amide dimers, erythrophlesins H-I (1, 2). Spectral data indicated that they consist of asymmetrical dimeric structure via an ester bond between two cassaine diterpenoids. MTT assay confirmed that compound 1, erythrophlesin H, had the strongest cytotoxic effect toward the human prostate cancer cell line, PC-3. The molecular mechanism by which this compound induced apoptosis cell in prostate cancer remains unknown. Erythrophlesin H induced apoptosis in a dose-dependent manner. Acridine orange and annexin V-FITC/PI double staining confirmed that erythrophlesin H effectively induces apoptosis in PC-3 cells.
authors.affiliation: College of Pharmacy, Catholic University of Daegu, Gyeongbuk 712-702, Republic of Korea.
authors.forename: Tran Manh
authors.initials: TM
authors.lastname: Hung
authors.affiliation: College of Pharmacy, Catholic University of Daegu, Gyeongbuk 712-702, Republic of Korea.
authors.forename: To Dao
authors.initials: TD
authors.lastname: Cuong
authors.affiliation: College of Pharmacy, Research Institute of Pharmaceutical Sciences, Kyungpook National University, Daegu 702-701, Republic of Korea.
authors.forename: Jeong Ah
authors.initials: JA
authors.lastname: Kim
authors.affiliation: College of Natural Science, Kangwon National University, Kangwon 200-701, Republic of Korea.
authors.forename: Jeong Hyung
authors.initials: JH
authors.lastname: Lee
authors.affiliation: College of Pharmacy, Catholic University of Daegu, Gyeongbuk 712-702, Republic of Korea.
authors.forename: Mi Hee
authors.initials: MH
authors.lastname: Woo
authors.affiliation: College of Pharmacy, Catholic University of Daegu, Gyeongbuk 712-702, Republic of Korea. Electronic address: bsmin@cu.ac.kr.
authors.forename: Byung Sun
authors.initials: BS
authors.lastname: Min
mesh_terms: D045784:Abietanes; D000470:Alkaloids / Q000737:chemistry*; D000577:Amides / Q000737:chemistry / Q000494:pharmacology*; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D004224:Diterpenes / Q000737:chemistry / Q000494:pharmacology*; D007887:Fabaceae / Q000737:chemistry*; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D015394:Molecular Structure; D011471:Prostatic Neoplasms / Q000188:drug therapy* / Q000473:pathology; D021241:Spectrometry, Mass, Electrospray Ionization; D014407:Tumor Cells, Cultured
chemical_list: D045784:Abietanes; D000470:Alkaloids; D000577:Amides; D000972:Antineoplastic Agents, Phytogenic; D004224:Diterpenes; C000599532:erythrophlesin H; C010696:cassaine
keywords: Cassaine diterpenoid amide; Erythrophlesin H; Erythrophleum fordii; Leguminosae; PC-3 apoptosis