title: Preparation of 5-fluorouracil-loaded nanoparticles and study of interaction with gastric cancer cells.
abstract: OBJECTIVE
  To prepare 5-fluorouracil (5-Fu) nanoparticles with higher encapsulation efficiency and drug loading, and then investigate interaction with the SGC-7901 gastric cancer cell line.
  
  
  METHODS
  Prescription was optimized by orthogonal experiments, the encapsulation efficiency and loading capacity were tested by high- performance liquid chromatography, and inhibition of proliferation by 5-Fu nanoparticles and 5-Fu given to cells for 24, 48 and 72 hours was investigated by methyl thiazolyl tetrazolium assay (MTT). In addition, 5-Fu nanoparticles were labeled by fluorescein isothiocyanate (FITC), and absorption into cells was tested by flow cytometry.
  
  
  RESULTS
  The optimal conditions for preparation were concentrations of 5-Fu of 5mg/ml, of CaCl2 of 60 mg/ml and of chitosan of 2 mg/ml. With a stirring speed of 1200rpm, encapsulation efficiency of 5-Fu nanoparticles was 55.4±1.10% and loading capacity was 4.22±0.14%; gastric cancer cells were significantly inhibited by 5-Fu nanoparticles in a time and concentration dependent manner, and compared to 5-Fu with slower drug release, in a certain concentration range, inhibition with 5-Fu nanoparticles was stronger. 5-Fu nanoparticles were absorbed by the cells in line with the concentration.
  
  
  CONCLUSIONS
  5-Fu nanoparticles can inhibit growth of gastric cancer cells in vitro to a greater extent than with 5-Fu with good adsorption characteristics, supporting feasibility as a carrier.
authors.affiliation: Harbin Commercial University Life Science and Environmental Science Research Center, Harbin, China E-mail : 429776474@qq.com.
authors.forename: Yu-Ling
authors.initials: YL
authors.lastname: Fan
authors.forename: Bing-Yu
authors.initials: BY
authors.lastname: Fan
authors.forename: Qiang
authors.initials: Q
authors.lastname: Li
authors.forename: Hai-Xiao
authors.initials: HX
authors.lastname: Di
authors.forename: Xiang-Yu
authors.initials: XY
authors.lastname: Meng
authors.forename: Na
authors.initials: N
authors.lastname: Ling
mesh_terms: D000964:Antimetabolites, Antineoplastic / Q000008:administration & dosage* / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D004337:Drug Carriers; D016503:Drug Delivery Systems; D005434:Flow Cytometry; D005472:Fluorouracil / Q000008:administration & dosage* / Q000494:pharmacology; D006801:Humans; D053758:Nanoparticles / Q000008:administration & dosage*; D013274:Stomach Neoplasms / Q000188:drug therapy* / Q000378:metabolism*; D014407:Tumor Cells, Cultured
chemical_list: D000964:Antimetabolites, Antineoplastic; D004337:Drug Carriers; D005472:Fluorouracil