title: Matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry using the Vitek MS system for rapid and accurate identification of dermatophytes on solid cultures.
abstract: The objective of this research was to extend the Vitek MS fungal knowledge base version 2.0.0 to allow the robust identification of clinically relevant dermatophytes, using a variety of strains, incubation times, and growth conditions. First, we established a quick and reliable method for sample preparation to obtain a reliable and reproducible identification independently of the growth conditions. The Vitek MS V2.0.0 fungal knowledge base was then expanded using 134 well-characterized strains belonging to 17 species in the genera Epidermophyton, Microsporum, and Trichophyton. Cluster analysis based on mass spectrum similarity indicated good species discrimination independently of the culture conditions. We achieved a good separation of the subpopulations of the Trichophyton anamorph of Arthroderma benhamiae and of anthropophilic and zoophilic strains of Trichophyton interdigitale. Overall, the 1,130 mass spectra obtained for dermatophytes gave an estimated identification performance of 98.4%. The expanded fungal knowledge base was then validated using 131 clinical isolates of dermatophytes belonging to 13 taxa. For 8 taxa all strains were correctly identified, and for 3 the rate of successful identification was >90%; 75% (6/8) of the M. gypseum strains were correctly identified, whereas only 47% (18/38) of the African T. rubrum population (also called T. soudanense) were recognized accurately, with a large quantity of strains misidentified as T. violaceum, demonstrating the close relationship of these two taxa. The method of sample preparation was fast and efficient and the expanded Vitek MS fungal knowledge base reliable and robust, allowing reproducible dermatophyte identifications in the routine laboratory.
authors.affiliation: Laboratory of Applied Microbiology, University of Applied Sciences and Arts of Southern Switzerland, Bellinzona, Switzerland sophie.derespinis@supsi.ch.
authors.forename: Sophie
authors.initials: S
authors.lastname: De Respinis
authors.affiliation: bioMérieux SA, Unit Microbiology, R&D Microbiology, La Balme Les Grottes, France.
authors.forename: Valérie
authors.initials: V
authors.lastname: Monnin
authors.affiliation: bioMérieux SA, Unit Microbiology, R&D Microbiology, La Balme Les Grottes, France.
authors.forename: Victoria
authors.initials: V
authors.lastname: Girard
authors.affiliation: bioMérieux SA, Unit Microbiology, R&D Microbiology, La Balme Les Grottes, France.
authors.forename: Martin
authors.initials: M
authors.lastname: Welker
authors.affiliation: bioMérieux SA, Unit Microbiology, R&D Microbiology, La Balme Les Grottes, France.
authors.forename: Maud
authors.initials: M
authors.lastname: Arsac
authors.affiliation: bioMérieux SA, Unit Microbiology, R&D Microbiology, La Balme Les Grottes, France.
authors.forename: Béatrice
authors.initials: B
authors.lastname: Cellière
authors.affiliation: bioMérieux SA, Unit Microbiology, R&D Microbiology, La Balme Les Grottes, France.
authors.forename: Géraldine
authors.initials: G
authors.lastname: Durand
authors.affiliation: Department of Dermatology, University Hospital of Zurich, Zurich, Switzerland.
authors.forename: Philipp P
authors.initials: PP
authors.lastname: Bosshard
authors.affiliation: Microbiology and Virology Unit, Papa Giovanni XXIII Hospital, Bergamo, Italy.
authors.forename: Claudio
authors.initials: C
authors.lastname: Farina
authors.affiliation: Microbiology and Virology Unit, Papa Giovanni XXIII Hospital, Bergamo, Italy.
authors.forename: Marco
authors.initials: M
authors.lastname: Passera
authors.affiliation: bioMérieux SA, Unit Microbiology, R&D Microbiology, La Balme Les Grottes, France.
authors.forename: Alex
authors.initials: A
authors.lastname: Van Belkum
authors.affiliation: POLE Pharma Consulting, Breganzona, Switzerland.
authors.forename: Orlando
authors.initials: O
authors.lastname: Petrini
authors.affiliation: Laboratory of Applied Microbiology, University of Applied Sciences and Arts of Southern Switzerland, Bellinzona, Switzerland.
authors.forename: Mauro
authors.initials: M
authors.lastname: Tonolla
mesh_terms: D003883:Arthrodermataceae / Q000737:chemistry* / Q000145:classification* / Q000302:isolation & purification; D016000:Cluster Analysis; D003881:Dermatomycoses / Q000175:diagnosis; D006801:Humans; D008828:Microbiological Techniques / Q000379:methods*; D009172:Mycology / Q000379:methods; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D013997:Time Factors