title: Isolation and characterization of poly(3-hydroxybutyrate)-producing bacteria from aerobic sludge.
abstract: Polyhydroxyalkanoates (PHAs) are biodegradable and environmentally friendly natural polymers. In this study, we isolated a bacterium strain capable of synthesizing PHAs from the aerobic sludge of a sewage treatment plant. The bacterium was identified as Burkholderia cepacia via physiological and biochemical tests as well as 16S rDNA sequence analysis. Strain WN-H41 produced PHAs, which was identified as P3HB. These PHAs have a number average molecular weight of 2.6 × 10(4) Da, a polydispersity index (PDI) of 2.4, and its thermal properties include a glass transition temperature of 1 °C, a melting temperature of 171 °C, and a decomposition temperature of 280 °C. These properties indicate that P3HB produced by WN-H41 has a high purity and good thermal stability.
authors.affiliation: Department of Environmental and Biological Engineering, School of Chemistry and Chemical Engineering/Key Laboratory for Green Processing of Chemical Engineering of Xinjiang Bingtuan, Shihezi University, Shihezi, 832000, China.
authors.forename: Bingxin
authors.initials: B
authors.lastname: Zheng
authors.forename: Jianjiang
authors.initials: J
authors.lastname: Lu
authors.forename: Yanbin
authors.initials: Y
authors.lastname: Tong
authors.forename: Hongling
authors.initials: H
authors.lastname: Li
authors.forename: Qianqian
authors.initials: Q
authors.lastname: Chen
mesh_terms: D020155:3-Hydroxybutyric Acid / Q000096:biosynthesis* / Q000737:chemistry; D000332:Aerobiosis; D016956:Burkholderia cepacia / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D002244:Carbon / Q000737:chemistry; D008970:Molecular Weight; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012722:Sewage / Q000382:microbiology*; D013696:Temperature
chemical_list: D012336:RNA, Ribosomal, 16S; D012722:Sewage; D002244:Carbon; D020155:3-Hydroxybutyric Acid