title: Increased meiotic crossovers and reduced genome stability in absence of Schizosaccharomyces pombe Rad16 (XPF).
abstract: Schizosaccharomyces pombe Rad16 is the ortholog of the XPF structure-specific endonuclease, which is required for nucleotide excision repair and implicated in the single strand annealing mechanism of recombination. We show that Rad16 is important for proper completion of meiosis. In its absence, cells suffer reduced spore viability and abnormal chromosome segregation with evidence for fragmentation. Recombination between homologous chromosomes is increased, while recombination within sister chromatids is reduced, suggesting that Rad16 is not required for typical homolog crossovers but influences the balance of recombination between the homolog and the sister. In vegetative cells, rad16 mutants show evidence for genome instability. Similar phenotypes are associated with mutants affecting Rhp14(XPA) but are independent of other nucleotide excision repair proteins such as Rad13(XPG). Thus, the XPF/XPA module of the nucleotide excision repair pathway is incorporated into multiple aspects of genome maintenance even in the absence of external DNA damage.
authors.affiliation: Program in Molecular and Computational BiologyUniversity of Southern California, Los Angeles, California 90089.
authors.forename: Tara L
authors.initials: TL
authors.lastname: Mastro
authors.affiliation: Program in Molecular and Computational BiologyUniversity of Southern California, Los Angeles, California 90089 forsburg@usc.edu.
authors.forename: Susan L
authors.initials: SL
authors.lastname: Forsburg
mesh_terms: D020090:Chromosome Segregation; D003434:Crossing Over, Genetic; D004249:DNA Damage; D004260:DNA Repair; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D004843:Epistasis, Genetic; D042822:Genomic Instability; D008540:Meiosis; D050296:Microbial Viability / Q000235:genetics; D009154:Mutation; D010641:Phenotype; D011995:Recombination, Genetic; D012568:Schizosaccharomyces / Q000235:genetics* / Q000378:metabolism; D029702:Schizosaccharomyces pombe Proteins / Q000235:genetics* / Q000378:metabolism; D013172:Spores, Fungal
chemical_list: D004268:DNA-Binding Proteins; C101945:RHP16 protein, S pombe; D029702:Schizosaccharomyces pombe Proteins
keywords: NER; Rad16; XPF; homologous recombination; meiosis