title: Expression of PTHrP and PTH/PTHrP receptor 1 in the superior cervical ganglia of rats.
abstract: PTHrP and its receptor PTHR1 are found in the CNS and peripheral nervous system. The presence of PTHrP mRNA has been detected in the superior cervical ganglion (SCG), but there are no data on the cellular distribution of PTHrP and PTHR1 in the SCG. Although it is known that ovarian activity and reproductive status influence sympathetic activity, and the PTHrP/PTHR1 system is influenced by estrogens in different tissues, it is not known whether these factors have a similar effect on expression of PTHrP and PTHR1 in the nervous system. Hence, we investigated the presence and distribution of PTHrP and PTHR1 in neurons and glia of the SCG of rats, as well as the influence of ovariectomy on their expression, by using immunohistochemistry. PTHrP and PTHR1 immunoreactivity was observed in cytoplasm as well as in nuclei of almost all neurons in the SCG. In male rats, intensity of PTHrP fluorescence was significantly higher in cytoplasm of NPY-, in comparison to NPY+ neurons (p < 0.05). In female rats, 2 months post-ovariectomy, significantly lower intensity of PTHrP fluorescence in cytoplasm of the SCG neurons was observed in comparison to sham operated animals (p < 0.05). In addition to neurons, PTHrP and PTHR1 immunoreactivity was observed in most of the glia and was not influenced by ovariectomy. Results show the expression of PTHrP and its receptor, PTHR1, in the majority of neurons and glial cells in the SCG of rats. Expression of PTHrP, but not PTHR1 in the cytoplasm of SCG neurons is influenced by ovarian activity.
authors.affiliation: Department of Anatomy, Histology and Embryology, University of Split School of Medicine, Šoltanska 2, 21000 Split, Croatia. Electronic address: natalija.filipovic@mefst.hr.
authors.forename: Natalija
authors.initials: N
authors.lastname: Filipović
authors.affiliation: Department of Anatomy, Histology and Embryology, University of Split School of Medicine, Šoltanska 2, 21000 Split, Croatia.
authors.forename: Marija
authors.initials: M
authors.lastname: Vrdoljak
authors.affiliation: Department of Anatomy, Histology and Embryology, University of Split School of Medicine, Šoltanska 2, 21000 Split, Croatia.
authors.forename: Ana
authors.initials: A
authors.lastname: Vuica
authors.affiliation: Department of Anatomy, Histology and Embryology, University of Split School of Medicine, Šoltanska 2, 21000 Split, Croatia.
authors.forename: Milka
authors.initials: M
authors.lastname: Jerić
authors.affiliation: Department of Anatomy, Histology and Embryology, University of Split School of Medicine, Šoltanska 2, 21000 Split, Croatia.
authors.forename: Antonia
authors.initials: A
authors.lastname: Jeličić Kadić
authors.affiliation: Department of Anatomy, Histology and Embryology, University of Split School of Medicine, Šoltanska 2, 21000 Split, Croatia.
authors.forename: Toni
authors.initials: T
authors.lastname: Utrobičić
authors.affiliation: Department of Animal Nutrition and Dietetics, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia.
authors.forename: Tomislav
authors.initials: T
authors.lastname: Mašek
authors.affiliation: Department of Anatomy, Histology and Embryology, University of Split School of Medicine, Šoltanska 2, 21000 Split, Croatia.
authors.forename: Ivica
authors.initials: I
authors.lastname: Grković
mesh_terms: D000818:Animals; D003593:Cytoplasm / Q000378:metabolism; D005260:Female; D008297:Male; D009457:Neuroglia / Q000378:metabolism; D009474:Neurons / Q000378:metabolism; D010052:Ovariectomy; D044162:Parathyroid Hormone-Related Protein / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D044168:Receptor, Parathyroid Hormone, Type 1 / Q000378:metabolism*; D017783:Superior Cervical Ganglion / Q000166:cytology / Q000378:metabolism*
chemical_list: D044162:Parathyroid Hormone-Related Protein; D044168:Receptor, Parathyroid Hormone, Type 1
keywords: Ovariectomy; PTHR1; PTHrP; Superior cervical ganglion