title: Microcellular open porous monoliths for cell growth by thiol-ene polymerization of low-toxicity monomers in high internal phase emulsions.
abstract: Open porous microcellular polymers with high degrees of porosity are prepared from divinyl adipate and pentaerythritol tetrakis(3-mercaptopropionate) by thiol-ene polymerization within high internal phase emulsions. The influence of monomer ratio, droplet phase volume, and emulsion stirring rate on the morphology and mechanical properties of the products is studied. The newly produced material is successfully applied as a scaffold for osteoblastic MC3T3-E1 cells in vitro, showing increased rates of cell growth compared to material prepared by standard methods.
authors.affiliation: Centre of Excellence PoliMaT, Tehnološki park 24, Ljubljana, Slovenia; Polymer Technology College, Ozare 19, Slovenj Gradec, Slovenia.
authors.forename: Maja
authors.initials: M
authors.lastname: Sušec
authors.forename: Robert
authors.initials: R
authors.lastname: Liska
authors.forename: Günter
authors.initials: G
authors.lastname: Russmüller
authors.forename: Jiři
authors.initials: J
authors.lastname: Kotek
authors.forename: Peter
authors.initials: P
authors.lastname: Krajnc
mesh_terms: D015097:3-Mercaptopropionic Acid / Q000031:analogs & derivatives* / Q000737:chemistry; D000272:Adipates / Q000737:chemistry*; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001709:Biotechnology / Q000379:methods*; D049109:Cell Proliferation / Q000502:physiology*; D004655:Emulsions / Q000737:chemistry; D008422:Materials Testing; D051379:Mice; D008855:Microscopy, Electron, Scanning; D010006:Osteoblasts / Q000166:cytology*; D011108:Polymers / Q000737:chemistry*; D016062:Porosity; D011409:Propylene Glycols / Q000737:chemistry*; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D000272:Adipates; D004655:Emulsions; D011108:Polymers; D011409:Propylene Glycols; C501435:pentaerythritol tetra(3-mercaptopropionate); C472390:divinyl adipate; D015097:3-Mercaptopropionic Acid; D000469:Alkaline Phosphatase
keywords: divinyl adipate; polyHIPE; porous polymers; thiol-ene polymerization; tissue engineering