title: NMR-based structural biology enhanced by dynamic nuclear polarization at high magnetic field.
abstract: Dynamic nuclear polarization (DNP) has become a powerful method to enhance spectroscopic sensitivity in the context of magnetic resonance imaging and nuclear magnetic resonance spectroscopy. We show that, compared to DNP at lower field (400 MHz/263 GHz), high field DNP (800 MHz/527 GHz) can significantly enhance spectral resolution and allows exploitation of the paramagnetic relaxation properties of DNP polarizing agents as direct structural probes under magic angle spinning conditions. Applied to a membrane-embedded K(+) channel, this approach allowed us to refine the membrane-embedded channel structure and revealed conformational substates that are present during two different stages of the channel gating cycle. High-field DNP thus offers atomic insight into the role of molecular plasticity during the course of biomolecular function in a complex cellular environment.
authors.affiliation: NMR Spectroscopy, Bijvoet Center for Biomolecular Research, Department of Chemistry, Faculty of Science, Utrecht University, 3584 CH, Utrecht, The Netherlands.
authors.forename: Eline J
authors.initials: EJ
authors.lastname: Koers
authors.forename: Elwin A W
authors.initials: EA
authors.lastname: van der Cruijsen
authors.forename: Melanie
authors.initials: M
authors.lastname: Rosay
authors.forename: Markus
authors.initials: M
authors.lastname: Weingarth
authors.forename: Alexander
authors.initials: A
authors.lastname: Prokofyev
authors.forename: Claire
authors.initials: C
authors.lastname: Sauvée
authors.forename: Olivier
authors.initials: O
authors.lastname: Ouari
authors.forename: Johan
authors.initials: J
authors.lastname: van der Zwan
authors.forename: Olaf
authors.initials: O
authors.lastname: Pongs
authors.forename: Paul
authors.initials: P
authors.lastname: Tordo
authors.forename: Werner E
authors.initials: WE
authors.lastname: Maas
authors.forename: Marc
authors.initials: M
authors.lastname: Baldus
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry*; D002247:Carbon Isotopes; D015640:Ion Channel Gating; D060526:Magnetic Fields; D019906:Nuclear Magnetic Resonance, Biomolecular; D015221:Potassium Channels / Q000737:chemistry*; D012997:Solvents; D013696:Temperature
chemical_list: D001426:Bacterial Proteins; D002247:Carbon Isotopes; D015221:Potassium Channels; D012997:Solvents; C107790:prokaryotic potassium channel