title: Distribution of classical enterotoxin genes in staphylococci from milk of cows with- and without mastitis and the cowshed environment.
abstract: The aim of this study was to analyze by PCR 185 isolates of Staphylococcus from milk of cows with- and without mastitis and from the cowsheds environment for their potential ability to produce five classical staphylococcal enterotoxins. Among S. aureus isolates 8 (32%) carried enterotoxin genes and only 2 of them had more than one gene. The enterotoxin genes were detected in 22 (13.7%) coagulase-negative staphylococci (CNS) isolates, among them in 9 (11.4%) isolates of S. xylosus, 5 (16.7%) S. sciuri, 3 (10.3%) S. epidermidis and in 5 (22.7%) Staphylococcus spp. In some CNS 2 or 3 genes were detected simultaneously. Among the investigated enterotoxin genes, sec was the most prevalent (70%). The genes encoding enterotoxin B and D were detected in 5 (16.7%) and 6 (20%) isolates, respectively. The lowest number of isolates had sea and see genes. The genes encoding enterotoxins were often identified in staphylococci from milk of cows with mastitis (73.4% of detected genes), while only 6 (20%) isolates from milk of cows without mastitis and 2 (6.6%) isolates from cowshed environment were positive for enterotoxin genes. The results showed that CNS from bovine milk, like S. aureus, carried enterotoxin genes and may pose a risk for public health.
authors.forename: M
authors.initials: M
authors.lastname: Piechota
authors.forename: B
authors.initials: B
authors.lastname: Kot
authors.forename: E
authors.initials: E
authors.lastname: Zdunek
authors.forename: J
authors.initials: J
authors.lastname: Mitrus
authors.forename: J
authors.initials: J
authors.lastname: Wicha
authors.forename: M K
authors.initials: MK
authors.lastname: Wolska
authors.forename: K
authors.initials: K
authors.lastname: Sachanowicz
mesh_terms: D000818:Animals; D002417:Cattle; D004269:DNA, Bacterial / Q000235:genetics; D004768:Enterotoxins / Q000235:genetics / Q000378:metabolism*; D005260:Female; D015964:Gene Expression Regulation, Bacterial; D008414:Mastitis, Bovine / Q000382:microbiology*; D016133:Polymerase Chain Reaction / Q000662:veterinary; D013210:Staphylococcus / Q000235:genetics / Q000378:metabolism*
chemical_list: D004269:DNA, Bacterial; D004768:Enterotoxins