title: Collagen-derived dipeptide prolyl-hydroxyproline promotes differentiation of MC3T3-E1 osteoblastic cells.
abstract: Prolyl-hydroxyproline (Pro-Hyp) is one of the major constituents of collagen-derived dipeptides. The objective of this study was to investigate the effects of Pro-Hyp on the proliferation and differentiation of MC3T3-E1 osteoblastic cells. Addition of Pro-Hyp did not affect MC3T3-E1 cell proliferation and matrix mineralization but alkaline phosphatase activity was significantly increased. Furthermore, cells treated with Pro-Hyp significantly upregulated gene expression of Runx2, Osterix, and Col1α1. These results indicate that Pro-Hyp promotes osteoblast differentiation. This study demonstrates for the first time that Pro-Hyp has a positive effect on osteoblast differentiation with upregulation of Runx2, Osterix, and Collα1 gene expression.
authors.affiliation: Faculty of Pharmaceutical Sciences, Josai University, 1-1 Keyakidai, Sakado, Saitama 350-0295, Japan. Electronic address: kimira@josai.ac.jp.
authors.forename: Yoshifumi
authors.initials: Y
authors.lastname: Kimira
authors.affiliation: Faculty of Pharmaceutical Sciences, Josai University, 1-1 Keyakidai, Sakado, Saitama 350-0295, Japan.
authors.forename: Kana
authors.initials: K
authors.lastname: Ogura
authors.affiliation: Faculty of Pharmaceutical Sciences, Josai University, 1-1 Keyakidai, Sakado, Saitama 350-0295, Japan.
authors.forename: Yuri
authors.initials: Y
authors.lastname: Taniuchi
authors.affiliation: Nitta Gelatin Inc., Peptide Division, 2-22 Futamata, Yao, Osaka 581-0024, Japan.
authors.forename: Aya
authors.initials: A
authors.lastname: Kataoka
authors.affiliation: Nitta Gelatin Inc., Peptide Division, 2-22 Futamata, Yao, Osaka 581-0024, Japan.
authors.forename: Naoki
authors.initials: N
authors.lastname: Inoue
authors.affiliation: Nitta Gelatin Inc., Peptide Division, 2-22 Futamata, Yao, Osaka 581-0024, Japan.
authors.forename: Fumihito
authors.initials: F
authors.lastname: Sugihara
authors.affiliation: Faculty of Pharmaceutical Sciences, Josai University, 1-1 Keyakidai, Sakado, Saitama 350-0295, Japan.
authors.forename: Sachie
authors.initials: S
authors.lastname: Nakatani
authors.affiliation: Faculty of Pharmaceutical Sciences, Josai University, 1-1 Keyakidai, Sakado, Saitama 350-0295, Japan.
authors.forename: Jun
authors.initials: J
authors.lastname: Shimizu
authors.affiliation: Faculty of Pharmaceutical Sciences, Josai University, 1-1 Keyakidai, Sakado, Saitama 350-0295, Japan.
authors.forename: Masahiro
authors.initials: M
authors.lastname: Wada
authors.affiliation: Faculty of Pharmaceutical Sciences, Josai University, 1-1 Keyakidai, Sakado, Saitama 350-0295, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Mano
mesh_terms: D016475:3T3 Cells; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D002113:Calcification, Physiologic; D002454:Cell Differentiation / Q000502:physiology*; D049109:Cell Proliferation; D003094:Collagen / Q000378:metabolism*; D024042:Collagen Type I / Q000378:metabolism; D050659:Core Binding Factor Alpha 1 Subunit / Q000235:genetics; D004151:Dipeptides / Q000378:metabolism / Q000502:physiology*; D051379:Mice; D010006:Osteoblasts / Q000166:cytology*; D060888:Real-Time Polymerase Chain Reaction; D000076182:Sp7 Transcription Factor; D014157:Transcription Factors / Q000235:genetics
chemical_list: D024042:Collagen Type I; D050659:Core Binding Factor Alpha 1 Subunit; D004151:Dipeptides; C493716:Runx2 protein, mouse; D000076182:Sp7 Transcription Factor; C479466:Sp7 protein, mouse; D014157:Transcription Factors; C533879:prolyl-4-hydroxyproline; D003094:Collagen; D000469:Alkaline Phosphatase
keywords: Collagen-derived peptide; Osteoblast differentiation; Prolyl-hydroxyproline