title: Protective effects of maslinic acid against alcohol-induced acute liver injury in mice.
abstract: Protective effects of maslinic acid (MA) at 10, 15 or 20 mg/kg body weight/day against alcohol-induced acute hepatotoxicity in mice were examined. Mice were administrated by MA for 3 weeks, and followed by alcohol treatment. Results showed that MA pre-intake at three doses resulted in its accumulation in the liver; and dose-dependently lowered cytochrome P450 2E1 activity and protein expression at 23.5-51.2% and 21.4-62.3%, respectively (P <0.05). MA pre-intake decreased subsequent alcohol-induced reactive oxygen species, interleukin-6, tumor necrosis factor-alpha, monocyte chemoattractant protein-1, nitric oxide and prostaglandin E2 production; retained glutathione content; maintained catalase and glutathione peroxidase activities; and declined cyclooxygenase-2 and total nitric oxide synthase activities in the liver (P <0.05). Furthermore, MA pre-intake suppressed 17.3-51.7% nuclear factor kappa (NF-κ)B p50, 23.5-58.8% NF-κB p65, 25.6-62.4% p-p38 and 24.1-63.0% p-JNK expression in the liver (P <0.05). Histological data indicated that MA intake at test doses attenuated hepatic inflammatory infiltrate. These findings support that maslinic acid is a potent preventive agent against acute alcoholic liver disease.
authors.affiliation: Division of Gastroenterology, Department of Internal Medicine, Chang Bing Show-Chwan Memorial Hospital, Changhua County, Taiwan.
authors.forename: Sheng-lei
authors.initials: SL
authors.lastname: Yan
authors.affiliation: Department of Nutrition, China Medical University, Taichung City, Taiwan.
authors.forename: Hui-ting
authors.initials: HT
authors.lastname: Yang
authors.affiliation: Department of Surgery, Chung Shan Medical University Hospital, Taichung City, Taiwan.
authors.forename: Hsiang-lin
authors.initials: HL
authors.lastname: Lee
authors.affiliation: Department of Nutrition, China Medical University, Taichung City, Taiwan; Department of Health and Nutrition Biotechnology, Asia University, Taichung City, Taiwan. Electronic address: mcyin@mail.cmu.edu.tw.
authors.forename: Mei-chin
authors.initials: MC
authors.lastname: Yin
mesh_terms: D000208:Acute Disease; D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D001835:Body Weight / Q000187:drug effects; D002492:Central Nervous System Depressants / Q000097:blood / Q000633:toxicity*; D016207:Cytokines / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D000431:Ethanol / Q000097:blood / Q000633:toxicity*; D008099:Liver / Q000378:metabolism / Q000473:pathology; D008108:Liver Diseases, Alcoholic / Q000473:pathology / Q000517:prevention & control*; D008111:Liver Function Tests; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D010944:Plants / Q000737:chemistry; D014315:Triterpenes / Q000032:analysis / Q000494:pharmacology*; D009828:Oleanolic Acid / Q000031:analogs & derivatives
chemical_list: D000975:Antioxidants; D002492:Central Nervous System Depressants; D016207:Cytokines; D000431:Ethanol; D014315:Triterpenes; C412811:maslinic acid; D009828:Oleanolic Acid
keywords: Alcohol; CYP2E1; Hepatotoxicity; MAPK; Maslinic acid; ROS