title: Functional G protein-coupled receptors on nuclei from brain and primary cultured neurons.
abstract: A growing number of G protein-coupled receptors (GPCRs) have been identified on nuclear membranes. In many cases, it is unknown how the intracellular GPCR is activated, how it is trafficked to nuclear membranes, and what long-term signaling consequences follow nuclear receptor activation. Here we describe how to isolate nuclei that are free from plasma membrane and cytoplasmic contamination yet still exhibit physiological properties following receptor activation.
authors.affiliation: Department of Anatomy and Neurobiology, Washington University School of Medicine, 660 South Euclid Ave, Saint Louis, MO, 63110, USA.
authors.forename: Yuh-Jiin I
authors.initials: YJ
authors.lastname: Jong
authors.forename: Karen L
authors.initials: KL
authors.lastname: O'Malley
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism; D002467:Cell Nucleus / Q000378:metabolism*; D057054:Molecular Imaging / Q000379:methods; D009474:Neurons / Q000378:metabolism*; D009685:Nuclear Envelope / Q000378:metabolism; D061251:Primary Cell Culture; D018160:Receptors, Cytoplasmic and Nuclear / Q000378:metabolism; D043562:Receptors, G-Protein-Coupled / Q000378:metabolism*; D015398:Signal Transduction
chemical_list: D018160:Receptors, Cytoplasmic and Nuclear; D043562:Receptors, G-Protein-Coupled; D002118:Calcium