title: Fabrication of electrochemical interface based on boronic acid-modified pyrroloquinoline quinine/reduced graphene oxide composites for voltammetric determination of glycated hemoglobin.
abstract: A voltammetric sensor for determination of glycated hemoglobin (HbA1c) was developed based on the composites of phenylboronic acid-modified pyrroloquinoline quinine (PBA-PQQ) and reduced graphene oxide. After the electrodeposition of reduced graphene oxide (ERGO) on the glassy carbon (GC) electrode, PQQ multilayer was decorated on the surface of the ERGO/GC electrode via potential cycling. Further modification with PBA would lead to the formation of the working electrode, namely PBA-PQQ/ERGO/GC electrode. PQQ on the electrode exhibited a quasi-reversible electrode process with 2-electron transfer and 2-proton participation, and the electron transfer efficiency was further enhanced by the introduction of ERGO layer. The complexation of PBA with HbA1c through specific boronic acid-diol recognition could cause the change of the oxidation peak current of PQQ on the electrode, which was utilized for HbA1c detection. Under the optimized conditions, the PBA-PQQ/ERGO/GC electrode provided high selectivity and high sensitivity for HbA1c detection with a linear range of 9.4-65.8 μg mL(-1) and a low detection limit of 1.25 μg mL(-1). The fabricated sensor was also successfully applied to determine the percentages of HbA1c in whole blood of healthy individuals.
authors.affiliation: Henan Key Laboratory Cultivation Base of Nanobiological Analytical Chemistry, College of Chemistry and Chemical Engineering, Shangqiu Normal University, Shangqiu 476000, China. Electronic address: zhouyanli@sqnc.edu.cn.
authors.forename: Yanli
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: College of Chemistry and Molecular Engineering, Zhengzhou University, Zhengzhou 450001, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Dong
authors.affiliation: Henan Key Laboratory Cultivation Base of Nanobiological Analytical Chemistry, College of Chemistry and Chemical Engineering, Shangqiu Normal University, Shangqiu 476000, China.
authors.forename: Lantao
authors.initials: L
authors.lastname: Liu
authors.affiliation: Henan Key Laboratory Cultivation Base of Nanobiological Analytical Chemistry, College of Chemistry and Chemical Engineering, Shangqiu Normal University, Shangqiu 476000, China.
authors.forename: Yuanqiang
authors.initials: Y
authors.lastname: Hao
authors.affiliation: Henan Key Laboratory Cultivation Base of Nanobiological Analytical Chemistry, College of Chemistry and Chemical Engineering, Shangqiu Normal University, Shangqiu 476000, China.
authors.forename: Zhu
authors.initials: Z
authors.lastname: Chang
authors.affiliation: Henan Key Laboratory Cultivation Base of Nanobiological Analytical Chemistry, College of Chemistry and Chemical Engineering, Shangqiu Normal University, Shangqiu 476000, China; College of Chemistry and Molecular Engineering, Zhengzhou University, Zhengzhou 450001, China. Electronic address: xumaotian@sqnc.edu.cn.
authors.forename: Maotian
authors.initials: M
authors.lastname: Xu
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation; D001774:Blood Chemical Analysis / Q000295:instrumentation*; D001897:Boronic Acids / Q000737:chemistry*; D003217:Conductometry / Q000295:instrumentation*; D004566:Electrodes; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006442:Glycated Hemoglobin / Q000032:analysis*; D006108:Graphite / Q000737:chemistry*; D006801:Humans; D010087:Oxides / Q000737:chemistry; D045542:PQQ Cofactor / Q000737:chemistry*
chemical_list: D001897:Boronic Acids; D006442:Glycated Hemoglobin; D006108:Graphite; D010087:Oxides; D045542:PQQ Cofactor
keywords: Boronic acid; Glycated hemoglobin; Pyrroloquinoline quinine; Reduced graphene oxide; Voltammetric sensor