title: [Human dirofilariasis: clinical and diagnostic signs and diagnostic methods].
abstract: The clinical and diagnostic signs caused by the tissue location and migration of adult Dirofilaria in the human body determine the use of different methods for the diagnosis of dirofilariasis. During their investigations, the authors modified polymerase chain reaction (PCR): they chose and synthesized primers and selected amplification regimens for them and obtained agarose gel bands that corresponded to PCR fragment length nucleotide sequences that were equal to 245 bp for D. (N.) repens and 656 bp for D. immitis. There was 100% agreement in the results of PCR and microscopic examination of sera from 32 dogs and 1 female patient with low parasitemia and in the blood nucleotide sequence characteristic of D. repens.
authors.forename: E N
authors.initials: EN
authors.lastname: Morozov
authors.forename: V G
authors.initials: VG
authors.lastname: Supriaga
authors.forename: V M
authors.initials: VM
authors.lastname: Rakova
authors.forename: L F
authors.initials: LF
authors.lastname: Morozova
authors.forename: L A
authors.initials: LA
authors.lastname: Zhukova
mesh_terms: D000818:Animals; D017931:DNA Primers / Q000138:chemical synthesis; D062109:Dermatologic Surgical Procedures; D004183:Dirofilaria immitis / Q000235:genetics / Q000302:isolation & purification*; D060348:Dirofilaria repens / Q000235:genetics / Q000302:isolation & purification*; D004184:Dirofilariasis / Q000175:diagnosis* / Q000469:parasitology / Q000473:pathology / Q000601:surgery; D004285:Dogs; D004587:Electrophoresis, Agar Gel; D005260:Female; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction / Q000379:methods*; D012867:Skin / Q000469:parasitology / Q000473:pathology
chemical_list: D017931:DNA Primers