title: Structure of an Aspergillus fumigatus old yellow enzyme (EasA) involved in ergot alkaloid biosynthesis.
abstract: The Aspergillus fumigatus old yellow enzyme (OYE) EasA reduces chanoclavine-I aldehyde to dihydrochanoclavine aldehyde and works in conjunction with festuclavine synthase at the branchpoint for ergot alkaloid pathways. The crystal structure of the FMN-loaded EasA was determined to 1.8 Å resolution. The active-site amino acids of OYE are conserved, supporting a similar mechanism for reduction of the α/β-unsaturated aldehyde. The C-terminal tail of one monomer packs into the active site of a monomer in the next asymmetric unit, which is most likely to be a crystallization artifact and not a mechanism of self-regulation.
authors.affiliation: Molecular Biosciences, University of Kansas, 1200 Sunnyside Avenue, Lawrence, KS 66045, USA.
authors.forename: Annemarie S
authors.initials: AS
authors.lastname: Chilton
authors.affiliation: Molecular Biosciences, University of Kansas, 1200 Sunnyside Avenue, Lawrence, KS 66045, USA.
authors.forename: Ashley L
authors.initials: AL
authors.lastname: Ellis
authors.affiliation: Molecular Biosciences, University of Kansas, 1200 Sunnyside Avenue, Lawrence, KS 66045, USA.
authors.forename: Audrey L
authors.initials: AL
authors.lastname: Lamb
mesh_terms: D001232:Aspergillus fumigatus / Q000201:enzymology*; D020134:Catalytic Domain; D018360:Crystallography, X-Ray; D004876:Ergot Alkaloids / Q000096:biosynthesis*; D005486:Flavin Mononucleotide / Q000737:chemistry; D005656:Fungal Proteins / Q000737:chemistry*; D008958:Models, Molecular; D009252:NADPH Dehydrogenase / Q000737:chemistry*; D017433:Protein Structure, Secondary
chemical_list: D004876:Ergot Alkaloids; D005656:Fungal Proteins; D005486:Flavin Mononucleotide; D009252:NADPH Dehydrogenase
keywords: Aspergillus fumigatus; EasA; FgaOx3; ergot alkaloid; old yellow enzyme