title: Biotin-deficient diet induces chromosome misalignment and spindle defects in mouse oocytes.
abstract: Increased abnormal oocytes due to meiotic chromosome misalignment and spindle defects lead to elevated rates of infertility, miscarriage, and trisomic conceptions. Here, we investigated the effect of biotin deficiency on oocyte quality. Three-week-old female ICR mice were fed a biotin-deficient or control diet (0, 0.004 g biotin/kg diet) for 21 days. On day 22, these mouse oocytes were analyzed by immunofluorescence. Due to biotin, undernutrition increased the frequency of abnormal oocytes (the biotin deficient vs. control: 40 vs. 16%). Next, the remaining mice in the biotin-deficient group were fed a control or biotin-deficient diet from day 22 to 42. Although biotin nutritional status in the recovery group was restored, the frequency of abnormal oocytes in the recovery group was still higher than that in the control group (48 vs. 18%). Our results indicate that steady, sufficient biotin intake is required for the production of high-quality oocytes in mice.
authors.affiliation: a Department of Nutrition , School of Human Cultures, The University of Shiga Prefecture , Hikone , Japan.
authors.forename: Ai
authors.initials: A
authors.lastname: Tsuji
authors.forename: Toshinobu
authors.initials: T
authors.lastname: Nakamura
authors.forename: Katsumi
authors.initials: K
authors.lastname: Shibata
mesh_terms: D000818:Animals; D001710:Biotin / Q000172:deficiency* / Q000652:urine; D001835:Body Weight; D002452:Cell Count; D002869:Chromosome Aberrations; D004032:Diet; D004435:Eating; D004958:Estradiol / Q000097:blood; D030762:Estrous Cycle; D005260:Female; D008099:Liver / Q000378:metabolism; D051379:Mice; D008813:Mice, Inbred ICR; D009865:Oocytes / Q000166:cytology* / Q000378:metabolism*; D009929:Organ Size; D010053:Ovary / Q000254:growth & development / Q000378:metabolism / Q000502:physiology; D008941:Spindle Apparatus; D014599:Uterus / Q000254:growth & development / Q000378:metabolism / Q000502:physiology; D014631:Valerates / Q000652:urine
chemical_list: D014631:Valerates; C004961:beta-hydroxyisovaleric acid; D004958:Estradiol; D001710:Biotin
keywords: biotin; deficiency; mouse; oocyte quality