title: Lysophosphatidylserine stimulates chemotactic migration of colorectal cancer cells through GPR34 and PI3K/Akt pathway.
abstract: BACKGROUND
  Lysophosphatidylserine (lysoPS) is a type of lysophospholipid mediator, which is involved in allergic conditions and tumor progression. We investigated the physiological function of lysoPS on colorectal cancer (CRC) cell lines, as well as the involved receptor and signaling pathways.
  
  
  METHODS
  Expression of lysoPS receptors on six cell lines was examined by reverse transcription-polymerase chain reaction (RT-PCR). The physiological functions of lysoPS were investigated, and experiments using small interfering RNA (siRNA) or inhibitors of the signaling pathways were conducted.
  
  
  RESULTS
  Among the three lysoPS receptors, GPR34 was highly expressed on all cell lines. LysoPS stimulated the chemotactic migratory ability. Wortmannin inhibited the migratory ability, as well as the GPR34 knock-down, strongly suggestive of the involvement of this receptor in the PI3K/Akt pathway.
  
  
  CONCLUSIONS
  The involved receptor and pathways in the migratory ability in response to lysoPS was demonstrated, which opens premises for targeting as a new strategy for prevention and treatment of colorectal cancer.
authors.affiliation: Department of Surgical Oncology, Faculty of Medical Sciences, The University of Tokyo, Tokyo, Japan yiida-tky@umin.ac.jp.
authors.forename: Yuuki
authors.initials: Y
authors.lastname: Iida
authors.affiliation: Department of Transfusion Medicine, Faculty of Medical Sciences, The University of Tokyo, Tokyo, Japan.
authors.forename: Nelson
authors.initials: N
authors.lastname: H Tsuno
authors.affiliation: Department of Surgical Oncology, Faculty of Medical Sciences, The University of Tokyo, Tokyo, Japan.
authors.forename: Junko
authors.initials: J
authors.lastname: Kishikawa
authors.affiliation: Department of Surgical Oncology, Faculty of Medical Sciences, The University of Tokyo, Tokyo, Japan.
authors.forename: Kensuke
authors.initials: K
authors.lastname: Kaneko
authors.affiliation: Department of Surgical Oncology, Faculty of Medical Sciences, The University of Tokyo, Tokyo, Japan.
authors.forename: Koji
authors.initials: K
authors.lastname: Murono
authors.affiliation: Department of Surgical Oncology, Faculty of Medical Sciences, The University of Tokyo, Tokyo, Japan.
authors.forename: Kazushige
authors.initials: K
authors.lastname: Kawai
authors.affiliation: Department of Transfusion Medicine, Faculty of Medical Sciences, The University of Tokyo, Tokyo, Japan.
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Ikeda
authors.affiliation: Department of Surgical Oncology, Faculty of Medical Sciences, The University of Tokyo, Tokyo, Japan.
authors.forename: Soichiro
authors.initials: S
authors.lastname: Ishihara
authors.affiliation: Department of Surgical Oncology, Faculty of Medical Sciences, The University of Tokyo, Tokyo, Japan.
authors.forename: Hironori
authors.initials: H
authors.lastname: Yamaguchi
authors.affiliation: Department of Surgical Oncology, Faculty of Medical Sciences, The University of Tokyo, Tokyo, Japan.
authors.forename: Eiji
authors.initials: E
authors.lastname: Sunami
authors.affiliation: Department of Surgical Oncology, Faculty of Medical Sciences, The University of Tokyo, Tokyo, Japan.
authors.forename: Joji
authors.initials: J
authors.lastname: Kitayama
authors.affiliation: Department of Clinical Laboratory Medicine, Faculty of Medical Sciences, The University of Tokyo, Tokyo, Japan.
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Yatomi
authors.affiliation: Department of Surgical Oncology, Faculty of Medical Sciences, The University of Tokyo, Tokyo, Japan.
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Watanabe
mesh_terms: D002448:Cell Adhesion / Q000187:drug effects; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects* / Q000235:genetics; D049109:Cell Proliferation / Q000187:drug effects; D015179:Colorectal Neoplasms / Q000235:genetics / Q000378:metabolism*; D016326:Extracellular Matrix Proteins / Q000378:metabolism; D055785:Gene Knockdown Techniques; D006801:Humans; D008246:Lysophospholipids / Q000494:pharmacology*; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism*; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism*; D049312:Receptors, Lysophospholipid / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects*
chemical_list: D016326:Extracellular Matrix Proteins; C118065:G-protein-coupled receptor 34; D008246:Lysophospholipids; D049312:Receptors, Lysophospholipid; C025059:lysophosphatidylserine; D019869:Phosphatidylinositol 3-Kinases; D051057:Proto-Oncogene Proteins c-akt
keywords: GPR34; Lysophosphatidylserine; chemotactic migration; colorectal cancer