title: Identification of functional cis-elements required for repression of the Taka-amylase A gene under secretion stress in Aspergillus oryzae.
abstract: The expression of secreted proteins in filamentous fungi is down-regulated by a transcriptional feedback mechanism under endoplasmic reticulum stress, termed repression under secretion stress (RESS). To investigate the RESS mechanism, we analyzed the expression of the Taka-amylase A gene (amyB) in Aspergillus oryzae, which was depressed under secreted protein stress. We conducted a truncation and deletion analysis of the amyB promoter to identify cis-elements required for RESS. A nucleotide sequence (positions -378 to -291) without any binding sites for the transcriptional activator AmyR, which is involved in amylolytic gene expression, was required for RESS. The octamer sequence TCACGGGC (positions -307 to -300) constituted the core sequence of the upstream activating element essential for amyB down-regulation under secretion stress. Both the inactivation of AmyR and RESS contributed to the down-regulation of amyB expression under ER stress.
authors.affiliation: School of Bioscience and Bioengineering, South China University of Technology, Guangzhou, People's Republic of China.
authors.forename: Bin
authors.initials: B
authors.lastname: Zhou
authors.forename: Chao
authors.initials: C
authors.lastname: Wang
authors.forename: Bin
authors.initials: B
authors.lastname: Wang
authors.forename: Xiupeng
authors.initials: X
authors.lastname: Li
authors.forename: Jing
authors.initials: J
authors.lastname: Xiao
authors.forename: Li
authors.initials: L
authors.lastname: Pan
mesh_terms: D001236:Aspergillus oryzae / Q000201:enzymology / Q000235:genetics*; D063185:Epigenetic Repression; D005656:Fungal Proteins / Q000235:genetics* / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics*; D013312:Stress, Physiological / Q000235:genetics; D000516:alpha-Amylases / Q000235:genetics* / Q000378:metabolism
chemical_list: D005656:Fungal Proteins; D000516:alpha-Amylases