title: Enzymatic amplification detection of peanut allergen Ara h1 using a stem-loop DNA biosensor modified with a chitosan-mutiwalled carbon nanotube nanocomposite and spongy gold film.
abstract: In this paper, a highly sensitive biosensor was constructed for peanut allergen Ara h1 detection. The biosensor was constructed by coating a glassy carbon electrode with a chitosan-mutiwalled carbon nanotube nanocomposite and then adding a spongy gold film via electro-deposition to increase the effective area. The probe switched from an "on" to an "off" state in the presence of target DNA, which detached biotin from the electrode surface. This also detached streptavidin-horseradish peroxidase (HRP-SA), which was bound to the electrode via specific interaction with biotin. The HRP-SA catalyzed chemical oxidation of hydroquinone by H2O2 to form benzoquinone, and when it was detached, electrochemical reduction of the signal of benzoquinone could be used to monitor DNA hybridization via chronoamperometry. Under optimum conditions, a wide dynamic detection range (3.91 × 10(-17)-1.25 × 10(-15) mol L(-1)) and a low detection limit (1.3 × 10(-17) mol L(-1)) were achieved for the complementary sequence. Furthermore, the DNA biosensor exhibited an excellent ability to discriminate between a complementary target and a one-base mismatch or non-complementary sequence. The sensor was successfully applied to Ara h1 analysis in peanuts.
authors.affiliation: State Key Laboratory of Food Science and Technology, School of Food Science of Jiangnan University, Synergetic Innovation Center of Food Safety, Wuxi 214122, Jiangsu, China. Electronic address: sxlzzz@jiangnan.edu.cn.
authors.forename: Xiulan
authors.initials: X
authors.lastname: Sun
authors.affiliation: State Key Laboratory of Food Science and Technology, School of Food Science of Jiangnan University, Synergetic Innovation Center of Food Safety, Wuxi 214122, Jiangsu, China.
authors.forename: Min
authors.initials: M
authors.lastname: Jia
authors.affiliation: State Key Laboratory of Food Science and Technology, School of Food Science of Jiangnan University, Synergetic Innovation Center of Food Safety, Wuxi 214122, Jiangsu, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Ji
authors.affiliation: State Key Laboratory of Food Science and Technology, School of Food Science of Jiangnan University, Synergetic Innovation Center of Food Safety, Wuxi 214122, Jiangsu, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Guan
authors.affiliation: State Key Laboratory of Food Science and Technology, School of Food Science of Jiangnan University, Synergetic Innovation Center of Food Safety, Wuxi 214122, Jiangsu, China.
authors.forename: Yinzhi
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Food Science and Technology, School of Food Science of Jiangnan University, Synergetic Innovation Center of Food Safety, Wuxi 214122, Jiangsu, China.
authors.forename: Lili
authors.initials: L
authors.lastname: Tang
authors.affiliation: School of Chemical and Material Engineering of Jiangnan University, Wuxi 214122, Jiangsu, China.
authors.forename: Zaijun
authors.initials: Z
authors.lastname: Li
mesh_terms: D000818:Animals; D052179:Antigens, Plant / Q000032:analysis*; D010367:Arachis / Q000737:chemistry; D015374:Biosensing Techniques / Q000379:methods*; D048271:Chitosan / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D055664:Electrochemical Techniques / Q000379:methods; D004566:Electrodes; D006023:Glycoproteins / Q000032:analysis*; D006046:Gold / Q000737:chemistry*; D006735:Horseradish Peroxidase / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000378:metabolism; D057230:Limit of Detection; D008565:Membrane Proteins; D053761:Nanocomposites / Q000737:chemistry*; D037742:Nanotubes, Carbon / Q000737:chemistry*; D009693:Nucleic Acid Hybridization; D010940:Plant Proteins / Q000032:analysis*; D011161:Porifera
chemical_list: D052179:Antigens, Plant; C500889:Ara h 1 protein, Arachis hypogaea; D006023:Glycoproteins; D008565:Membrane Proteins; D037742:Nanotubes, Carbon; D010940:Plant Proteins; D006046:Gold; D004247:DNA; D048271:Chitosan; D006861:Hydrogen Peroxide; D006735:Horseradish Peroxidase
keywords: Arachishypogaea allergen 1 (Ara h1); Chitosan-mutiwalled carbon nanotube nanocomposite; Electrochemical biosensor; Peanut allergen; Spongy gold film; Stem-loop probe