title: In vitro bioactivity and antimicrobial tuning of bioactive glass nanoparticles added with neem (Azadirachta indica) leaf powder.
abstract: Silica and phosphate based bioactive glass nanoparticles (58SiO2-33CaO-9P2O5) with doping of neem (Azadirachta indica) leaf powder and silver nanoparticles were prepared and characterised. Bioactive glass nanoparticles were produced using sol-gel technique. In vitro bioactivity of the prepared samples was investigated using simulated body fluid. X-ray diffraction (XRD) pattern of prepared glass particles reveals amorphous phase and spherical morphology with a particle size of less than 50 nm. When compared to neem doped glass, better bioactivity was attained in silver doped glass through formation of hydroxyapatite layer on the surface, which was confirmed through XRD, Fourier transform infrared (FTIR), and scanning electron microscopy (SEM) analysis. However, neem leaf powder doped bioactive glass nanoparticles show good antimicrobial activity against Staphylococcus aureus and Escherichia coli and less bioactivity compared with silver doped glass particles. In addition, the biocompatibility of the prepared nanocomposites reveals better results for neem doped and silver doped glasses at lower concentration. Therefore, neem doped bioactive glass may act as a potent antimicrobial agent for preventing microbial infection in tissue engineering applications.
authors.affiliation: Centre for Nanoscience and Technology, K. S. Rangasamy College of Technology, Tiruchengode, Namakkal District, Tamil Nadu 637215, India.
authors.forename: M
authors.initials: M
authors.lastname: Prabhu
authors.affiliation: Centre for Nanoscience and Technology, K. S. Rangasamy College of Technology, Tiruchengode, Namakkal District, Tamil Nadu 637215, India.
authors.forename: S
authors.initials: S
authors.lastname: Ruby Priscilla
authors.affiliation: Centre for Nanoscience and Technology, K. S. Rangasamy College of Technology, Tiruchengode, Namakkal District, Tamil Nadu 637215, India.
authors.forename: K
authors.initials: K
authors.lastname: Kavitha
authors.affiliation: Centre for Nanoscience and Technology, K. S. Rangasamy College of Technology, Tiruchengode, Namakkal District, Tamil Nadu 637215, India.
authors.forename: P
authors.initials: P
authors.lastname: Manivasakan
authors.affiliation: Centre for Nanoscience and Technology, K. S. Rangasamy College of Technology, Tiruchengode, Namakkal District, Tamil Nadu 637215, India.
authors.forename: V
authors.initials: V
authors.lastname: Rajendran
authors.affiliation: Department of Mechanical Engineering, K. S. Rangasamy College of Technology, Tiruchengode, Namakkal District, Tamil Nadu 637215, India.
authors.forename: P
authors.initials: P
authors.lastname: Kulandaivelu
mesh_terms: D000890:Anti-Infective Agents / Q000494:pharmacology*; D031601:Azadirachta / Q000737:chemistry*; D001826:Body Fluids / Q000187:drug effects; D045744:Cell Line, Tumor; D048430:Cell Shape / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004926:Escherichia coli / Q000187:drug effects; D005898:Glass / Q000737:chemistry*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008826:Microbial Sensitivity Tests; D053758:Nanoparticles / Q000648:ultrastructure; D018515:Plant Leaves / Q000737:chemistry*; D011208:Powders; D012834:Silver / Q000494:pharmacology; D013052:Spectrometry, X-Ray Emission; D017550:Spectroscopy, Fourier Transform Infrared; D013211:Staphylococcus aureus / Q000187:drug effects; D014961:X-Ray Diffraction
chemical_list: D000890:Anti-Infective Agents; D011208:Powders; D012834:Silver