title: Comprehensive quantification of triacylglycerols in soybean seeds by electrospray ionization mass spectrometry with multiple neutral loss scans.
abstract: Soybean seeds are an important source of vegetable oil and biomaterials. The content of individual triacylglycerol species (TAG) in soybean seeds is difficult to quantify in an accurate and rapid way. The present study establishes an approach to quantify TAG species in soybean seeds utilizing an electrospray ionization tandem mass spectrometry with multiple neutral loss scans. Ten neutral loss scans were performed to detect the fatty acyl chains of TAG, including palmitic (P, 16:0), linolenic (Ln, 18:3), linoleic (L, 18:2), oleic (O, 18:1), stearic (S, 18:0), eicosadienoic (20:2), gadoleic (20:1), arachidic (20:0), erucic (22:1), and behenic (22:0). The abundance of ten fatty acyl chains at 46 TAG masses (mass-to-charge ratio, m/z) were determined after isotopic deconvolution and correction by adjustment factors at each TAG mass. The direct sample infusion and multiple internal standards correction allowed a rapid and accurate quantification of TAG species. Ninety-three TAG species were resolved and their levels were determined. The most abundant TAG species were LLL, OLL, LLLn, PLL, OLLn, OOL, POL, and SLL. Many new species were detected and quantified. This shotgun lipidomics approach should facilitate the study of TAG metabolism and genetic breeding of soybean seeds for desirable TAG content and composition.
authors.affiliation: 1] Department of Biology, University of Missouri, St. Louis, Missouri 63121 [2] Donald Danforth Plant Science Center, St. Louis, Missouri 63132.
authors.forename: Maoyin
authors.initials: M
authors.lastname: Li
authors.affiliation: 1] Department of Biology, University of Missouri, St. Louis, Missouri 63121 [2] Donald Danforth Plant Science Center, St. Louis, Missouri 63132.
authors.forename: Emily
authors.initials: E
authors.lastname: Butka
authors.affiliation: 1] Department of Biology, University of Missouri, St. Louis, Missouri 63121 [2] Donald Danforth Plant Science Center, St. Louis, Missouri 63132.
authors.forename: Xuemin
authors.initials: X
authors.lastname: Wang
mesh_terms: D010938:Plant Oils / Q000737:chemistry*; D012639:Seeds / Q000737:chemistry; D013025:Glycine max / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization; D014280:Triglycerides / Q000737:chemistry* / Q000302:isolation & purification
chemical_list: D010938:Plant Oils; D014280:Triglycerides