title: Identification of a pentatricopeptide repeat RNA editing factor in Physcomitrella patens chloroplasts.
abstract: The moss Physcomitrella patens has two RNA editing sites in the chloroplasts. Here we identified a novel DYW-subclass pentatricopeptide repeat (PPR) protein, PpPPR_45, as a chloroplast RNA editing factor in P. patens. Knockdown of the PpPPR_45 gene reduced the extent of RNA editing at the chloroplast rps14-C2 site, whereas over-expression of PpPPR_45 increased the levels of RNA editing at both the rps14-C2 site and its neighboring C site. This indicates that the expression level of PpPPR_45 affects the extent of RNA editing at the two neighboring sites.
authors.affiliation: Center for Gene Research, Nagoya University, Chikusa-ku, Nagoya 464-8602, Japan.
authors.forename: Mizuho
authors.initials: M
authors.lastname: Ichinose
authors.affiliation: Center for Gene Research, Nagoya University, Chikusa-ku, Nagoya 464-8602, Japan.
authors.forename: Masato
authors.initials: M
authors.lastname: Uchida
authors.affiliation: Center for Gene Research, Nagoya University, Chikusa-ku, Nagoya 464-8602, Japan. Electronic address: sugita@gene.nagoya-u.ac.jp.
authors.forename: Mamoru
authors.initials: M
authors.lastname: Sugita
mesh_terms: D000595:Amino Acid Sequence; D019068:Bryopsida / Q000235:genetics / Q000378:metabolism*; D002736:Chloroplasts / Q000378:metabolism*; D055785:Gene Knockdown Techniques; D008969:Molecular Sequence Data; D010788:Photosynthesis; D010940:Plant Proteins / Q000737:chemistry* / Q000378:metabolism*; D021381:Protein Transport; D017393:RNA Editing; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020449:Repetitive Sequences, Amino Acid
chemical_list: D010940:Plant Proteins; D012333:RNA, Messenger
keywords: Chloroplast; DYW domain; Pentatricopeptide repeat protein; Physcomitrella patens; RNA editing