title: Ruthenium (II) complexes of thiosemicarbazone: synthesis, biosensor applications and evaluation as antimicrobial agents.
abstract: A conformationally rigid half-sandwich organoruthenium (II) complex [(η(6)-p-cymene)RuClTSC(N-S)]Cl, (1) and carbonyl complex [Ru(CO)Cl(PPh3)2TSC(N-S)] (2) have been synthesized from the reaction of [{(η(6)-p-cymene)RuCl}2(μ-Cl)2] and [Ru(H)(Cl)(CO)(PPh3)3] with thiophene-2-carboxaldehyde thiosemicarbazon (TSC) respectively and both novel ruthenium (II) complexes have been characterized by elemental analysis, FT-IR and NMR spectroscopy. The peripheral TSC in the complexes acts as an electrochemical coupling unit providing the ability to carry out electrochemical deposition (ED) and to form an electro-deposited film on a graphite electrode surface. The biosensing applicability of complexes 1 and 2 was investigated by using glucose oxidase (GOx) as a model enzyme. Electrochemical measurements at -0.9V versus Ag/AgCl electrode by following the ED Ru(II) reduction/oxidation due to from the enzyme activity, in the presence of glucose substrate. The designed biosensor showed a very good linearity for 0.01-0.5mM glucose. The in vitro antimicrobial activities of complexes 1 and 2 were also investigated against nine bacterial strains and one fungus by the disc diffusion test method. No activity was observed against the Gram-negative strains and fungus, whereas complex 1 showed moderate antibacterial activities against Gram-positive bacterial strains.
authors.affiliation: Dokuz Eylul University, The Graduate School of Natural and Applied Sciences, Department of Chemistry, 35160 Buca, Izmir, Turkey.
authors.forename: Hatice
authors.initials: H
authors.lastname: Yildirim
authors.affiliation: Ege University, Faculty of Science, Department of Biochemistry, 35100 Bornova, Izmir, Turkey.
authors.forename: Emine
authors.initials: E
authors.lastname: Guler
authors.affiliation: Ege University, Faculty of Science, Department of Biochemistry, 35100 Bornova, Izmir, Turkey; Dicle University, Faculty of Science, Department of Chemistry, 21280 Diyarbakir, Turkey. Electronic address: myavuz@dicle.edu.tr.
authors.forename: Murat
authors.initials: M
authors.lastname: Yavuz
authors.affiliation: Dokuz Eylul University, The Graduate School of Natural and Applied Sciences, Department of Chemistry, 35160 Buca, Izmir, Turkey.
authors.forename: Nurdan
authors.initials: N
authors.lastname: Ozturk
authors.affiliation: Dokuz Eylul University, The Graduate School of Natural and Applied Sciences, Department of Chemistry, 35160 Buca, Izmir, Turkey.
authors.forename: Pelin
authors.initials: P
authors.lastname: Kose Yaman
authors.affiliation: Dokuz Eylul University, Faculty of Science, Department of Chemistry, 35160 Buca, Izmir, Turkey.
authors.forename: Elif
authors.initials: E
authors.lastname: Subasi
authors.affiliation: Dokuz Eylul University, Faculty of Science, Department of Chemistry, 35160 Buca, Izmir, Turkey.
authors.forename: Elif
authors.initials: E
authors.lastname: Sahin
authors.affiliation: Ege University, Faculty of Science, Department of Biochemistry, 35100 Bornova, Izmir, Turkey; Ege University, Institute on Drug Abuse, Toxicology and Pharmaceutical Science (BATI), 35100 Bornova, Izmir, Turkey.
authors.forename: Suna
authors.initials: S
authors.lastname: Timur
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000494:pharmacology; D000935:Antifungal Agents / Q000737:chemistry* / Q000494:pharmacology; D015374:Biosensing Techniques; D055664:Electrochemical Techniques; D005658:Fungi / Q000187:drug effects; D005949:Glucose Oxidase / Q000737:chemistry; D006090:Gram-Negative Bacteria / Q000187:drug effects; D006094:Gram-Positive Bacteria / Q000187:drug effects; D009682:Magnetic Resonance Spectroscopy; D008968:Molecular Conformation; D012428:Ruthenium / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D013882:Thiosemicarbazones / Q000737:chemistry*
chemical_list: D000900:Anti-Bacterial Agents; D000935:Antifungal Agents; D013882:Thiosemicarbazones; D012428:Ruthenium; D005949:Glucose Oxidase
keywords: Antimicrobial activity; Biosensor; Enzyme immobilization; Ruthenium; Thiosemicarbazone