title: Sulfated polysaccharides from Loligo vulgaris skin: potential biological activities and partial purification.
abstract: The characteristics, biological properties, and purification of sulfated polysaccharides extracted from squid (Loligo vulgaris) skin were investigated. Their chemical and physical characteristics were determined using X-ray diffraction and infrared spectroscopic analysis. Sulfated polysaccharides from squid skin (SPSS) contained 85.06% sugar, 2.54% protein, 1.87% ash, 8.07% sulfate, and 1.72% uronic acid. The antioxidant properties of SPSS were investigated based on DPPH radical-scavenging capacity (IC50 = 19.42 mg mL(-1)), hydrogen peroxide-scavenging activity (IC50 = 0.91 mg mL(-1)), and β-carotene bleaching inhibition (IC50 = 2.79 mg mL(-1)) assays. ACE-inhibitory activity of SPSS was also investigated (IC50 = 0.14 mg mL(-1)). Further antimicrobial activity assays indicated that SPSS exhibited marked inhibitory activity against the bacterial and fungal strains tested. Those polysaccharides did not display hemolytic activity towards bovine erythrocytes. Fractionation by DEAE-cellulose column chromatography showed three major absorbance peaks. Results of this study suggest that sulfated polysaccharides from squid skin are attractive sources of polysaccharides and promising candidates for future application as dietary ingredients.
authors.forename: Baha Eddine
authors.initials: BE
authors.lastname: Abdelmalek
authors.forename: Assaâd
authors.initials: A
authors.lastname: Sila
authors.forename: Fatma
authors.initials: F
authors.lastname: Krichen
authors.forename: Wafa
authors.initials: W
authors.lastname: Karoud
authors.forename: Oscar
authors.initials: O
authors.lastname: Martinez-Alvarez
authors.forename: Semia
authors.initials: S
authors.lastname: Ellouz-Chaabouni
authors.forename: Mohamed Ali
authors.initials: MA
authors.lastname: Ayadi
authors.forename: Ali
authors.initials: A
authors.lastname: Bougatef
mesh_terms: D000806:Angiotensin-Converting Enzyme Inhibitors / Q000494:pharmacology; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D000935:Antifungal Agents / Q000494:pharmacology; D000975:Antioxidants / Q000494:pharmacology; D002417:Cattle; D002848:Chromatography, DEAE-Cellulose; D002852:Chromatography, Ion Exchange; D016166:Free Radical Scavengers / Q000494:pharmacology; D006461:Hemolysis / Q000187:drug effects; D006861:Hydrogen Peroxide / Q000737:chemistry; D049834:Loligo / Q000737:chemistry*; D008826:Microbial Sensitivity Tests; D007703:Peptidyl-Dipeptidase A / Q000378:metabolism; D011134:Polysaccharides / Q000302:isolation & purification* / Q000494:pharmacology*; D012867:Skin / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D013431:Sulfates / Q000302:isolation & purification* / Q000494:pharmacology*; D014961:X-Ray Diffraction; D019207:beta Carotene / Q000737:chemistry
chemical_list: D000806:Angiotensin-Converting Enzyme Inhibitors; D000900:Anti-Bacterial Agents; D000935:Antifungal Agents; D000975:Antioxidants; D016166:Free Radical Scavengers; D011134:Polysaccharides; D013431:Sulfates; D019207:beta Carotene; D006861:Hydrogen Peroxide; D007703:Peptidyl-Dipeptidase A