title: Isolating primary melanocyte-like cells from the mouse heart.
abstract: We identified a novel population of melanocyte-like cells (also known as cardiac melanocytes) in the hearts of mice and humans that contribute to atrial arrhythmia triggers in mice. To investigate the electrical and biological properties of cardiac melanocytes we developed a procedure to isolate them from mouse hearts that we derived from those designed to isolate neonatal murine cardiomyocytes. In order to obtain healthier cardiac melanocytes suitable for more extensive patch clamp or biochemical studies, we developed a refined procedure for isolating and plating cardiac melanocytes based on those originally designed to isolate cutaneous melanocytes. The refined procedure is demonstrated in this review and produces larger numbers of healthy melanocyte-like cells that can be plated as a pure population or with cardiomyocytes.
authors.affiliation: Penn Cardiovascular Institute, Perelman School of Medicine, University of Pennsylvania.
authors.forename: Hayoung
authors.initials: H
authors.lastname: Hwang
authors.affiliation: Penn Cardiovascular Institute, Perelman School of Medicine, University of Pennsylvania.
authors.forename: Fang
authors.initials: F
authors.lastname: Liu
authors.affiliation: Penn Cardiovascular Institute, Perelman School of Medicine, University of Pennsylvania.
authors.forename: Mark D
authors.initials: MD
authors.lastname: Levin
authors.affiliation: Penn Cardiovascular Institute, Perelman School of Medicine, University of Pennsylvania ; patelv@mail.med.upenn.edu.
authors.forename: Vickas V
authors.initials: VV
authors.lastname: Patel
mesh_terms: D000818:Animals; D001281:Atrial Fibrillation / Q000473:pathology; D003584:Cytological Techniques / Q000379:methods*; D005260:Female; D006801:Humans; D008297:Male; D008544:Melanocytes / Q000166:cytology* / Q000473:pathology; D051379:Mice; D009206:Myocardium / Q000166:cytology* / Q000473:pathology