title: [Study of sensitivity of laboratory animals to a causative agent of argentine hemorrhagic fever].
abstract: OBJECTIVE
  Study sensitivity of laboratory animals to a causative agent ofArgentine hemorrhagic fever.
  
  
  METHODS
  Junin virus strain XJ P37 was obtained from the State Collection of Causative Agents of Viral Hemorrhagic Fevers of the Pathogenicity Group I of Scientific Research Center of the 33rd Central Scientific Research Test Institute (SRC of the 33rd CSRTI). Junin virus strain XJ P37 culture with biological activity of 5.2 1g PFU x ml was used in the experiments. Mice (2 - 4 and 7 - 14 days old), guinea pigs (250 - 300 g), 1.8 - 2.5 kg shinshilla breed rabbits, 2.0 - 3.0 kg javanese macaque monkeys were obtained from vivarium of the SRC of the 33rd CSRTI. Vero (B) and GMK-AH-1 (D) cell cultures were obtained from cell culture collection of the SRC of the 33rd CSRTI. Biological activity calculation of Junin virus was carried out by Kerber in I.P. Amsharin modification.
  
  
  RESULTS
  Lethality in animals was from 12.5 to 50% after intranasal and intraperitoneal infection of guinea pigs, intramuscular, intraperitoneal and subcutaneous infection of rabbits, intracerebral and intranasal infection of mice at the doses from 0.4 to 1.0 x 10(5) PFU. Death of infected monkeys after intramuscular administration of the virus at 1.0 x 10(4) PFU dose was not observed. In 100% of surviving animals formation of virus-neutralizing antibodies was registered.
  
  
  CONCLUSIONS
  Evaluation of sensitivity of laboratory animals to Junin virus has shown that intracerebrally infected mice may be used to maintain causative agent culture, infected guinea pigs - to prepare virus-containing cultures and modelling infection exacerbation in humans. Intramuscularly infected rabbits may be used to obtain hyper-immune sera.
authors.forename: S I
authors.initials: SI
authors.lastname: Syromiatnikova
authors.forename: V B
authors.initials: VB
authors.lastname: Pantiukhov
authors.forename: I V
authors.initials: IV
authors.lastname: Shatokhina
authors.forename: M A
authors.initials: MA
authors.lastname: Timofeev
authors.forename: T E
authors.initials: TE
authors.lastname: Sizikova
authors.forename: S V
authors.initials: SV
authors.lastname: Borisevich
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000302:isolation & purification; D006477:Arenaviruses, New World / Q000472:pathogenicity*; D004195:Disease Models, Animal; D006168:Guinea Pigs; D006478:Hemorrhagic Fever, American / Q000453:epidemiology / Q000473:pathology / Q000821:virology*; D006801:Humans; D018051:Junin virus / Q000472:pathogenicity*; D051379:Mice; D011817:Rabbits
chemical_list: D000914:Antibodies, Viral