title: Novel locus for fibrinogen in 3' region of LEPR gene in island population of Vis (Croatia).
abstract: Leptin, a possible mediator between energy homeostasis, inflammation and cardiovascular disease (CVD), acts via leptin receptors. We investigated association of single-nucleotide polymorphisms (SNPs) and haplotypes of the leptin receptor gene (LEPR) with several CVD risk factors: body mass index, waist circumference (WC), serum lipids, fibrinogen and C-reactive protein levels. Thirty-one SNPs in and near LEPR gene were analyzed in 986 inhabitants of the island of Vis, Croatia and 29 SNPs in the inland sample (N=499). We assessed linkage disequilibrium (LD), SNP and haplotype associations with the selected phenotypes. rs4291477 significantly associated with fibrinogen (P=0.003) and rs7539471 marginally significantly with high-density lipoprotein (P=0.004), but only in the Vis sample, while rs10493384 marginally significantly associated with triglyceride levels (P=0.006) in the inland sample. SNPs were grouped into eight LD blocks in Vis and in seven blocks in the inland population. Haplotype A-C-A-A-G-A in block 5 in Vis (rs1782754, rs1171269, rs1022981, rs6673324, rs3790426, rs10493380) and haplotype A-A-A-A in block 4 in the inland data (rs1782754, rs1022981, rs6673324, rs1137100) were nominally associated with WC, P=7.085 × 10(-22) (adjusted P=0.0979) and P=5.496 × 10(-144) (adjusted P=0.1062), respectively.
authors.affiliation: Institute for Anthropological Research, Gajeva 32, Zagreb, Croatia.
authors.forename: Željka
authors.initials: Ž
authors.lastname: Tomas
authors.affiliation: Institute for Anthropological Research, Gajeva 32, Zagreb, Croatia.
authors.forename: Matea Zajc
authors.initials: MZ
authors.lastname: Petranović
authors.affiliation: Institute for Anthropological Research, Gajeva 32, Zagreb, Croatia.
authors.forename: Tatjana
authors.initials: T
authors.lastname: Škarić-Jurić
authors.affiliation: Institute for Anthropological Research, Gajeva 32, Zagreb, Croatia.
authors.forename: Ana
authors.initials: A
authors.lastname: Barešić
authors.affiliation: Institute for Anthropological Research, Gajeva 32, Zagreb, Croatia.
authors.forename: Marijana Peričić
authors.initials: MP
authors.lastname: Salihović
authors.affiliation: Institute for Anthropological Research, Gajeva 32, Zagreb, Croatia.
authors.forename: Nina Smolej
authors.initials: NS
authors.lastname: Narančić
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015992:Body Mass Index; D002097:C-Reactive Protein / Q000378:metabolism; D002318:Cardiovascular Diseases / Q000097:blood / Q000235:genetics; D017523:Croatia; D005260:Female; D005340:Fibrinogen / Q000378:metabolism*; D005787:Gene Frequency; D005828:Genetics, Population / Q000379:methods*; D006239:Haplotypes; D006801:Humans; D062312:Islands; D015810:Linkage Disequilibrium; D008055:Lipids / Q000097:blood; D008297:Male; D008875:Middle Aged; D010641:Phenotype; D020641:Polymorphism, Single Nucleotide; D054411:Receptors, Leptin / Q000235:genetics*; D012307:Risk Factors; D055105:Waist Circumference; D055815:Young Adult
chemical_list: D008055:Lipids; D054411:Receptors, Leptin; D005340:Fibrinogen; D002097:C-Reactive Protein