title: Aggregation of whey protein hydrolysate using Alcalase 2.4 L.
abstract: Here, we describe peptide aggregation, which is also known as enzymatic protein resynthesis. Whey protein hydrolysate (WPH) is the starting material for assembling peptides. Analyses of the involved amino acids, intrinsic fluorescence, fluorescence phase diagram, secondary structure, turbidity, and surface hydrophobicity were performed to investigate the reaction process. The aggregation mechanism consists of two parts: 1) formation and 2) aggregation of the building blocks that form the ordered secondary β-sheet structure. Constructing the building blocks requires at least one intermediate state, which is formed after 0.5 hours. Non-synergistic changes in the secondary and tertiary structures then allow the intermediate state to emerge.
authors.affiliation: Department of Applied Chemistry, College of Science, Northeast Agricultural University, Harbin, China.
authors.forename: Chunhong
authors.initials: C
authors.lastname: Liu
authors.affiliation: Department of Applied Chemistry, College of Science, Northeast Agricultural University, Harbin, China.
authors.forename: Wen
authors.initials: W
authors.lastname: Liu
authors.affiliation: Department of Applied Chemistry, College of Science, Northeast Agricultural University, Harbin, China.
authors.forename: Zhibiao
authors.initials: Z
authors.lastname: Feng
authors.affiliation: Department of Applied Chemistry, College of Science, Northeast Agricultural University, Harbin, China.
authors.forename: Dongmei
authors.initials: D
authors.lastname: Li
mesh_terms: D000596:Amino Acids / Q000737:chemistry; D057927:Hydrophobic and Hydrophilic Interactions; D010455:Peptides / Q000737:chemistry*; D066329:Protein Aggregates; D011492:Protein Hydrolysates / Q000737:chemistry*; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D013381:Subtilisins / Q000737:chemistry*; D000067816:Whey Proteins / Q000737:chemistry*
chemical_list: D000596:Amino Acids; D010455:Peptides; D066329:Protein Aggregates; D011492:Protein Hydrolysates; D000067816:Whey Proteins; D013381:Subtilisins