title: Crossover formation during rice meiosis relies on interaction of OsMSH4 and OsMSH5.
abstract: MSH4 encodes a MutS protein that plays a specialized role in meiosis. In eukaryotic species, such as budding yeast, mice, Caenorhabditis elegans, and Arabidopsis, msh4 mutants display meiotic defects with a reduced number of chiasmata. Here, we characterized rice MSH4 by map-based cloning. In Osmsh4 mutants, the chiasma frequency was dramatically decreased to ∼10% of the wild type, but the synaptonemal complex was normally installed. The double mutant analysis showed that in the Osmsh4 Osmsh5 mutant, the reduction of chiasmata was greater than other zmm mutants. This was consistent with the absence of localization for OsZIP4 and OsMER3 in Osmsh4 and suggests an earlier role for OsMSH4 and OsMSH5 than other ZMM proteins where they may be required to stabilize progenitor Holliday junctions. Using yeast two-hybrid and pull-down assays, we verified the direct physical association between OsMSH4 and OsMSH5 and OsMSH5 and HEI10 in plants for the first time. The MSH4-MSH5 heterodimer has been demonstrated in mammals to stabilize the formation of progenitor and double Holliday junctions that may be resolved as crossovers (COs). We propose that OsMSH4 interacts with OsMSH5 to promote formation of the majority of COs in rice.
authors.affiliation: State Key Laboratory of Plant Genomics and Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Plant Genomics and Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Ding
authors.initials: D
authors.lastname: Tang
authors.affiliation: Ministry of Education Key Laboratory of Agriculture Biodiversity for Plant Disease Management, Yunnan Agricultural University, Kunming 650201, China.
authors.forename: Qiong
authors.initials: Q
authors.lastname: Luo
authors.affiliation: State Key Laboratory of Plant Genomics and Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China Ningxia Academy of Agriculture and Forestry Sciences Agricultural Biotechnology Center, Yinchuan 750002, China.
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Plant Genomics and Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Hongjun
authors.initials: H
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Plant Genomics and Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Yafei
authors.initials: Y
authors.lastname: Li
authors.affiliation: State Key Laboratory of Plant Genomics and Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China zkcheng@genetics.ac.cn.
authors.forename: Zhukuan
authors.identifier: http://orcid.org/0000-0001-8428-8010
authors.initials: Z
authors.lastname: Cheng
mesh_terms: D032461:Chromosomes, Plant; D003001:Cloning, Molecular; D003434:Crossing Over, Genetic; D005838:Genotype; D008540:Meiosis; D009154:Mutation; D012275:Oryza / Q000235:genetics* / Q000378:metabolism*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding; D021381:Protein Transport
chemical_list: D010940:Plant Proteins
keywords: OsMSH4; crossover; meiosis; rice