title: Krüppel-like factor 9 inhibits glioma cell proliferation and tumorigenicity via downregulation of miR-21.
abstract: Krüppel-like factors (KLFs) are zinc finger-containing transcription factors that play key roles in the regulation of differentiation and development as well as biological processes central to the development of malignancies. Increasing evidence indicates that Krüppel-like factor 9 (KLF9) plays a critical role in regulating tumorigenesis. However, the biological role and molecular mechanism of KLF9 in glioma progression remain unclear. Herein, we found that KLF9 expression was strongly reduced in gliomas. Reduced KLF9 expression promoted glioma cell proliferation. Importantly, re-constitution of KLF9 expression inhibited glioma cell proliferation and tumor growth in vivo. Furthermore, we determined that KLF9 interacted with the miR-21 promoter, leading to suppression of miR-21 expression and cell cycle arrest. Taken together, our findings indicate a novel mechanism for KLF function in tumorigenesis and may also suggest new targets for clinical intervention in human cancer.
authors.affiliation: Department of Neurosurgery, Institute of Regeneration of Guangdong, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China.
authors.forename: Shuyun
authors.initials: S
authors.lastname: Huang
authors.affiliation: Department of The Central Laboratory, The First Affiliated Hospital/School of Clinical Medicine of GuangDong Pharmaceutical University, Guangzhou 510080, China.
authors.forename: Chanjuan
authors.initials: C
authors.lastname: Wang
authors.affiliation: Department of Neurosurgery, Institute of Regeneration of Guangdong, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China.
authors.forename: Yongjun
authors.initials: Y
authors.lastname: Yi
authors.affiliation: Department of Neurosurgery, Institute of Regeneration of Guangdong, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China.
authors.forename: Xinlin
authors.initials: X
authors.lastname: Sun
authors.affiliation: Department of Neurosurgery, Institute of Regeneration of Guangdong, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China.
authors.forename: Minjie
authors.initials: M
authors.lastname: Luo
authors.affiliation: Department of Neurosurgery, Institute of Regeneration of Guangdong, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China.
authors.forename: Zhenjun
authors.initials: Z
authors.lastname: Zhou
authors.affiliation: Department of Neurosurgery, Institute of Regeneration of Guangdong, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China.
authors.forename: Jianwen
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Neurosurgery, Institute of Regeneration of Guangdong, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China.
authors.forename: Yingqian
authors.initials: Y
authors.lastname: Cai
authors.affiliation: Department of Neurosurgery, Institute of Regeneration of Guangdong, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China.
authors.forename: Xiaodan
authors.initials: X
authors.lastname: Jiang
authors.affiliation: Department of Neurosurgery, Institute of Regeneration of Guangdong, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China. Electronic address: yiquanke9@sina.com.
authors.forename: Yiquan
authors.initials: Y
authors.lastname: Ke
mesh_terms: D000818:Animals; D017209:Apoptosis; D001253:Astrocytes / Q000378:metabolism; D015153:Blotting, Western; D002448:Cell Adhesion; D049109:Cell Proliferation; D002471:Cell Transformation, Neoplastic / Q000473:pathology*; D005434:Flow Cytometry; D015972:Gene Expression Regulation, Neoplastic; D005910:Glioma / Q000235:genetics* / Q000378:metabolism / Q000473:pathology*; D006801:Humans; D007124:Immunoenzyme Techniques; D051741:Kruppel-Like Transcription Factors / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D035683:MicroRNAs / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014407:Tumor Cells, Cultured; D023041:Xenograft Model Antitumor Assays
chemical_list: C489676:KLF9 protein, human; D051741:Kruppel-Like Transcription Factors; C524940:MIRN21 microRNA, human; D035683:MicroRNAs; D012333:RNA, Messenger
keywords: Glioma; KLF9; Zinc finger-containing transcription factors; miR-21; microRNA