title: Capillary electrophoresis with laser-induced fluorescence detection for studying amino acid uptake by yeast during beer fermentation.
abstract: The amino acid composition of cultivation broth is known to affect the biomass accumulation, productivity, and vitality of yeast during cultivation. A separation method based on capillary electrophoresis with laser-induced fluorescence (LIF) detection was developed for the determination of amino acid consumption by Saccharomyces cerevisiae during beer fermentation. Intraday relative standard deviations were less than 2.1% for migration times and between 2.9% and 9.9% for peak areas. Interday relative standard deviations were less than 2.5% for migration times and between 4.4% and 18.9% for peak areas. The quantification limit was even as low as 62.5 pM which equals to below attomole level detection. The method was applied to study the rate of amino acid utilization during beer fermentation.
authors.affiliation: VTT Technical Research Centre of Finland, PO Box 1000, FIN-02044 VTT, Espoo, Finland; Lappeenranta University of Technology, PO Box 20, FIN-53580 Lappeenranta, Finland. Electronic address: heidi.turkia@vtt.fi.
authors.forename: Heidi
authors.initials: H
authors.lastname: Turkia
authors.affiliation: University of Helsinki, Department of Chemistry, PO Box 55, University of Helsinki, FIN-00014 Helsinki, Finland.
authors.forename: Heli
authors.initials: H
authors.lastname: Sirén
authors.affiliation: VTT Technical Research Centre of Finland, PO Box 1000, FIN-02044 VTT, Espoo, Finland.
authors.forename: Merja
authors.initials: M
authors.lastname: Penttilä
authors.affiliation: VTT Technical Research Centre of Finland, PO Box 1000, FIN-02044 VTT, Espoo, Finland.
authors.forename: Juha-Pekka
authors.initials: JP
authors.lastname: Pitkänen
mesh_terms: D000596:Amino Acids / Q000032:analysis* / Q000378:metabolism*; D001515:Beer / Q000032:analysis*; D018533:Biomass; D019075:Electrophoresis, Capillary / Q000379:methods*; D005285:Fermentation / Q000502:physiology*; D005453:Fluorescence; D007834:Lasers; D057230:Limit of Detection; D012441:Saccharomyces cerevisiae / Q000254:growth & development / Q000378:metabolism*
chemical_list: D000596:Amino Acids
keywords: Amino acids; Beer fermentation; Bioprocess monitoring; Capillary electrophoresis; Laser-induced fluorescence detection