title: Evaluation of gold nanoparticle based lateral flow assays for diagnosis of enterobacteriaceae members in food and water.
abstract: Lateral flow immunoassays (LFIAs) are advantageous over conventional detection methods in terms of their simplicity and rapidity. These assays have been reported using various types of labels but colloidal gold nanoparticles are still the preferred choice as a label because of their easy synthesis, visual detection and stability. Bacterial contamination of food and drinking water is a major threat and hindrance towards ensuring food and water safety. Enterobacteriaceae family members are mainly transmitted by the consumption of contaminated water and food and implicated in various food or water borne infections. The LFIAs have been popularly used for detection of bacterial cells in different matrices. Therefore, this review intends to provide an analysis of the gold nanoparticle based lateral flow assays developed for detecting enterobacteriaceae family members in food and water samples. The review includes detailed data and discusses the factors that influence the performance of LFIAs and their shortcomings.
authors.affiliation: Department of Biotechnology, Motilal Nehru National Institute of Technology, Allahabad 211004, U.P., India.
authors.forename: Jyoti
authors.initials: J
authors.lastname: Singh
authors.affiliation: Department of Biotechnology, Motilal Nehru National Institute of Technology, Allahabad 211004, U.P., India.
authors.forename: Shivesh
authors.initials: S
authors.lastname: Sharma
authors.affiliation: Department of Biotechnology, Motilal Nehru National Institute of Technology, Allahabad 211004, U.P., India. Electronic address: seemanara@mnnit.ac.in.
authors.forename: Seema
authors.initials: S
authors.lastname: Nara
mesh_terms: D001431:Bacteriological Techniques / Q000592:standards*; D004269:DNA, Bacterial / Q000032:analysis; D004755:Enterobacteriaceae / Q000235:genetics / Q000302:isolation & purification*; D005516:Food Microbiology; D006046:Gold / Q000737:chemistry*; D007118:Immunoassay; D053768:Metal Nanoparticles / Q000737:chemistry*; D016133:Polymerase Chain Reaction; D014871:Water Microbiology
chemical_list: D004269:DNA, Bacterial; D006046:Gold
keywords: E. coli; Enterobacteriaceae; Gold nanoparticle; Lateral flow immunoassay; Salmonella