title: Proline isomerization of the immune receptor-interacting protein RIN4 by a cyclophilin inhibits effector-triggered immunity in Arabidopsis.
abstract: In the absence of pathogen infection, plant effector-triggered immune (ETI) receptors are maintained in a preactivation state by intermolecular interactions with other host proteins. Pathogen effector-induced alterations activate the receptor. In Arabidopsis, the ETI receptor RPM1 is activated via bacterial effector AvrB-induced phosphorylation of the RPM1-interacting protein RIN4 at Threonine 166. We find that RIN4 also interacts with the prolyl-peptidyl isomerase (PPIase) ROC1, which is reduced upon RIN4 Thr166 phosphorylation. ROC1 suppresses RPM1 immunity in a PPIase-dependent manner. Consistent with this, RIN4 Pro149 undergoes cis/trans isomerization in the presence of ROC1. While the RIN4(P149V) mutation abolishes RPM1 resistance, the deletion of Pro149 leads to RPM1 activation in the absence of RIN4 phosphorylation. These results support a model in which RPM1 directly senses conformational changes in RIN4 surrounding Pro149 that is controlled by ROC1. RIN4 Thr166 phosphorylation indirectly regulates RPM1 resistance by modulating the ROC1-mediated RIN4 isomerization.
authors.affiliation: College of Life Sciences, Peking University, No. 5 YiheYuan Road, Beijing 100871, China; State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, CAS, No. 1 West Beichen Road, Beijing 100101, China.
authors.forename: Meng
authors.initials: M
authors.lastname: Li
authors.affiliation: Center for Plant Biology, MOE Key Laboratory of Bioinformatics, Tsinghua Yuan 1, School of Life Sciences, Beijing 100084, China.
authors.forename: Xiqing
authors.initials: X
authors.lastname: Ma
authors.affiliation: Department of Plant Pathology, University of California, Davis, Davis, CA 95616, USA.
authors.forename: Yi-Hsuan
authors.initials: YH
authors.lastname: Chiang
authors.affiliation: Department of Plant Pathology, University of California, Davis, Davis, CA 95616, USA.
authors.forename: Koste A
authors.initials: KA
authors.lastname: Yadeta
authors.affiliation: College of Life Sciences, Peking University, No. 5 YiheYuan Road, Beijing 100871, China.
authors.forename: Pengfei
authors.initials: P
authors.lastname: Ding
authors.affiliation: State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, CAS, No. 1 West Beichen Road, Beijing 100101, China.
authors.forename: Liansai
authors.initials: L
authors.lastname: Dong
authors.affiliation: State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, CAS, No. 1 West Beichen Road, Beijing 100101, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, CAS, No. 1 West Beichen Road, Beijing 100101, China.
authors.forename: Xiuming
authors.initials: X
authors.lastname: Li
authors.affiliation: State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, CAS, No. 1 West Beichen Road, Beijing 100101, China.
authors.forename: Yufei
authors.initials: Y
authors.lastname: Yu
authors.affiliation: Center for Molecular Agrobiology and State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, CAS, No. 1 West Beichen Road, Beijing 100101, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Center for Molecular Agrobiology and State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, CAS, No. 1 West Beichen Road, Beijing 100101, China.
authors.forename: Qian-Hua
authors.initials: QH
authors.lastname: Shen
authors.affiliation: College of Life Sciences, Peking University, No. 5 YiheYuan Road, Beijing 100871, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Xia
authors.affiliation: Department of Plant Pathology, University of California, Davis, Davis, CA 95616, USA.
authors.forename: Gitta
authors.initials: G
authors.lastname: Coaker
authors.affiliation: Center for Plant Biology, MOE Key Laboratory of Bioinformatics, Tsinghua Yuan 1, School of Life Sciences, Beijing 100084, China. Electronic address: liu-d@mail.tsinghua.edu.cn.
authors.forename: Dong
authors.initials: D
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, CAS, No. 1 West Beichen Road, Beijing 100101, China. Electronic address: jmzhou@genetics.ac.cn.
authors.forename: Jian-Min
authors.initials: JM
authors.lastname: Zhou
mesh_terms: D017360:Arabidopsis / Q000276:immunology* / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000378:metabolism*; D002352:Carrier Proteins / Q000378:metabolism*; D021983:Cyclophilins / Q000378:metabolism*; D047908:Intracellular Signaling Peptides and Proteins; D007536:Isomerism; D011392:Proline / Q000378:metabolism*; D011485:Protein Binding
chemical_list: D029681:Arabidopsis Proteins; D002352:Carrier Proteins; D047908:Intracellular Signaling Peptides and Proteins; C453279:RIN4 protein, Arabidopsis; D011392:Proline; D021983:Cyclophilins; C515035:ROC1 protein, Arabidopsis