title: Preparation, identfication, and activity assay of lamprey (lampetra japonica) natural intelectins.
abstract: Intelectins play an important role in innate immune response. In a previous study, lamprey inteletins purified by galactose-Sepharose were inactive and insoluble. Herein, we provided a simple and effective method to purify natural intelectins from the serum of lamprey (Lethenteron japonicum) using proteinG agarose. SDS-PAGE, two-dimensional polyacrylamide gel electrophoresis (2D-PAGE), and mass spectrometry (MS) were used to analyze the purified proteins. The purified proteins were identified to be lamprey serum lectin and intelectinB. The activity analysis results indicated that the proteins had certain extent agglutination activity. The effective method will be useful to study their immune functions and molecular mechanisms.
authors.affiliation: a College of Life Science , Liaoning Normal University , Dalian , China.
authors.forename: Peng
authors.initials: P
authors.lastname: Su
authors.forename: Zhen
authors.initials: Z
authors.lastname: Zheng
authors.forename: Yue
authors.initials: Y
authors.lastname: Pang
authors.forename: Zhuang
authors.initials: Z
authors.lastname: Xue
authors.forename: Meng
authors.initials: M
authors.lastname: Gou
authors.forename: Yinglun
authors.initials: Y
authors.lastname: Han
authors.forename: Ge
authors.initials: G
authors.lastname: Liu
authors.forename: Qi
authors.initials: Q
authors.lastname: Zan
authors.forename: Qingwei
authors.initials: Q
authors.lastname: Li
mesh_terms: D000372:Agglutination Tests; D000818:Animals; D016207:Cytokines / Q000097:blood / Q000494:pharmacology; D015180:Electrophoresis, Gel, Two-Dimensional; D004912:Erythrocytes / Q000187:drug effects; D004926:Escherichia coli / Q000187:drug effects; D029941:Fish Proteins / Q000097:blood / Q000494:pharmacology; D058851:GPI-Linked Proteins / Q000097:blood / Q000494:pharmacology; D007798:Lampreys / Q000378:metabolism; D037102:Lectins / Q000097:blood / Q000494:pharmacology; D011817:Rabbits; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D016207:Cytokines; D029941:Fish Proteins; D058851:GPI-Linked Proteins; D037102:Lectins
keywords: immunoprecipitation; innate immune; intelectin; lamprey; mass spectrometry; purification