title: Significance of mutations in hepatitis B virus X gene for the pathogenesis of HB-associated glomerulonephritis.
abstract: In this study, the significance of hepatitis virus (HBV) X gene mutations for the pathogenesis of HBV-associated glomerulonephritis (HBV-GN) was investigated. DNA was extracted from 50 HBV-GN patients and 60 asymptomatic HBV carriers and subjected to PCR amplification and sequencing of HBV X gene. In HBV-GN patients, missense nucleotide mutations of C1653T, A1726C, A1727T, C1730G, T1753C, A1762T, and G1764A were detected in 84% of subjects, all located in the trans-acting regulatory region of the X gene. In control patients, missense nucleotide mutations of A1632C and A1635C were detected in 8% of subjects, both located in the non-functional region of the X gene. We conclude that, in most HBV-GN patients, X gene missense mutations occurred at some key sites playing an important role in the pathogenesis of HBV-GN.
authors.forename: D
authors.initials: D
authors.lastname: Hui
authors.forename: X
authors.initials: X
authors.lastname: Yan
authors.forename: J
authors.initials: J
authors.lastname: Wei
authors.forename: M
authors.initials: M
authors.lastname: Ruixia
authors.forename: G
authors.initials: G
authors.lastname: Guangju
mesh_terms: D016022:Case-Control Studies; D005838:Genotype; D005921:Glomerulonephritis / Q000209:etiology / Q000821:virology*; D006509:Hepatitis B / Q000150:complications* / Q000821:virology; D006515:Hepatitis B virus / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D020125:Mutation, Missense; D015534:Trans-Activators / Q000235:genetics* / Q000378:metabolism; D054334:Viral Regulatory and Accessory Proteins
chemical_list: D015534:Trans-Activators; D054334:Viral Regulatory and Accessory Proteins; C057628:hepatitis B virus X protein