title: Molecular cloning and expression analysis of the MTN gene during adventitious root development in IBA-induced tetraploid black locust.
abstract: 5'-Methylthioadenosine (MTA) nucleosidase (MTN) plays a key role in the methionine (Met) recycling pathway of plants. Here, we report the isolation of the 1158 bp full-length, cDNA sequence encoding tetraploid black locust (Robinia pseudoacacia L.) MTN (TrbMTN), which contains an open reading frame of 810 bp that encodes a 269 amino acid protein. The amino acid sequence of TrbMTN has more than 88% sequence identity to the MTNs from other plants, with a closer phylogenetic relationship to MTNs from legumes than to MTNs from other plants. Subcellular localization analysis revealed that the TrbMTN gene localizes mainly to the cell membrane and cytoplasm of onion epidermal cells. Indole-3-butyric acid (IBA)-treated cuttings showed higher TrbMTN transcript levels than untreated control cuttings during root primordium and adventitious root formation. TrbMTN and key Met cycle genes showed differential expression in shoots, leaves, stems, and roots, with the highest expression observed in stems. IBA-treated cuttings also showed higher TrbMTN activity than control cuttings during root primordium and adventitious root formation. These results indicate that TrbMTN gene might play an important role in the regulation of IBA-induced adventitious root development in tetraploid black locust cuttings.
authors.affiliation: State Key Laboratory of Soil Erosion and Dryland Farming on the Loess Plateau, Northwest A&F University, Yangling 712100, China.
authors.forename: Jine
authors.initials: J
authors.lastname: Quan
authors.affiliation: State Key Laboratory of Soil Erosion and Dryland Farming on the Loess Plateau, Northwest A&F University, Yangling 712100, China.
authors.forename: Chunxia
authors.initials: C
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Soil Erosion and Dryland Farming on the Loess Plateau, Northwest A&F University, Yangling 712100, China.
authors.forename: Sheng
authors.initials: S
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Soil Erosion and Dryland Farming on the Loess Plateau, Northwest A&F University, Yangling 712100, China.
authors.forename: Sen
authors.initials: S
authors.lastname: Meng
authors.affiliation: State Key Laboratory of Soil Erosion and Dryland Farming on the Loess Plateau, Northwest A&F University, Yangling 712100, China. Electronic address: zhaozhlunwen2010@126.com.
authors.forename: Zhong
authors.initials: Z
authors.lastname: Zhao
authors.affiliation: State Key Laboratory of Soil Erosion and Dryland Farming on the Loess Plateau, Northwest A&F University, Yangling 712100, China.
authors.forename: Xuexuan
authors.initials: X
authors.lastname: Xu
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D017931:DNA Primers; D007211:Indoles / Q000494:pharmacology*; D008969:Molecular Sequence Data; D009699:N-Glycosyl Hydrolases / Q000737:chemistry / Q000145:classification / Q000235:genetics*; D010802:Phylogeny; D018517:Plant Roots / Q000187:drug effects* / Q000254:growth & development; D016133:Polymerase Chain Reaction; D011123:Polyploidy; D031301:Robinia / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D013347:Subcellular Fractions / Q000201:enzymology
chemical_list: D017931:DNA Primers; D007211:Indoles; C014612:indolebutyric acid; D009699:N-Glycosyl Hydrolases
keywords: 5′-Methylthioadenosine nucleosidase; Adventitious root development; IBA; Tetraploid black locust