title: In vivo calcium recordings and channelrhodopsin-2 activation through an optical fiber.
abstract: We describe here an approach for the fluorometric monitoring of population activity in neurons in live mice combined with the activation of optogenetic actuators in vivo. In this protocol, a thin multimode fiber, which is used for both delivering excitation light and collecting emitted fluorescence signals, is inserted into the skull of a mouse. When combined with multicell bolus loading of Ca(2+) indicators, this optical fiber and its associated fluorescence detection system can be used for the in vivo recording of brain Ca(2+) signals from a local cluster of coactive neurons. The fiber can also be used for the optogenetic stimulation of light-activated ion channels, such as channelrhodopsin-2, allowing the monitoring of local calcium signals evoked by optogenetic stimulation.
authors.forename: Helmuth
authors.initials: H
authors.lastname: Adelsberger
authors.forename: Christine
authors.initials: C
authors.lastname: Grienberger
authors.forename: Albrecht
authors.initials: A
authors.lastname: Stroh
authors.forename: Arthur
authors.initials: A
authors.lastname: Konnerth
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism*; D000075402:Channelrhodopsins; D005336:Fiber Optic Technology / Q000295:instrumentation / Q000379:methods; D051379:Mice; D008822:Mice, Transgenic; D062308:Optogenetics
chemical_list: D000075402:Channelrhodopsins; D002118:Calcium