title: Optical coherence tomography and histopathology of macular uveitis.
abstract: OBJECTIVE
  Spectral-domain optical coherence tomography (SD-OCT) is a noninvasive technique that can provide high-resolution images of macular morphology. The purpose of this study was to examine the pathological mechanism of uveitis and compare the changes in the macula of uveitis patients and the histopathological features of experimentally induced uveitis in mice.
  
  
  METHODS
  Macular OCT was performed on 78 eyes of 51 patients of the Eye Hospital of Shandong University of Traditional Chinese Medicine, China, with apparent uveitis changes. C57BL/6 mice were injected with interphotoreceptor retinoid-binding protein (IRBP)-specific T cells from naïve mice immunized with complete Freund adjuvant IRBP(1-20) to induce uveitis. The disease was monitored by indirect fundoscopy. The eyes of the mice with experimental autoimmune uveitis (EAU) were enucleated 18 days after injection and classified according to pathological characteristics.
  
  
  RESULTS
  The characteristics of uveitis were classified into six categories. Macular edema was detected in 48 eyes (61.5%); macular epiretinal membrane in 22 eyes (28.2%); choroidal neovascularization and macular lamellar holes in 4 eyes (5.1%), respectively; macular atrophy in 10 eyes (12.8%); and serous neuroepithelium detachment in 22 eyes (28.2%). As in human patients, pathological examinations of mouse EAU showed inflammation, folds, and atrophy of the outer part of the neuroretina, choroidal neovascularization with hemorrhagic retinal detachment, serous neuroepithelium detachment, and epiretinal membrane formation.
  
  
  CONCLUSIONS
  Macular OCT of uveitis patients can display different morphological characteristics. Mouse EAU can simulate human uveitis. The comparative analysis of macular OCT in human uveitis and transfer EAU histopathology changes could provide important information on the pathogenesis of human uveitis.
authors.affiliation: *MD, PhD †MD The Affiliated Eye Hospital of Shandong University of Traditional Chinese Medicine, Jinan, China (all authors).
authors.forename: Yan
authors.initials: Y
authors.lastname: Cui
authors.forename: Guimin
authors.initials: G
authors.lastname: Wang
authors.forename: Yang
authors.initials: Y
authors.lastname: Li
authors.forename: Ying
authors.initials: Y
authors.lastname: Wang
authors.forename: Xingrong
authors.initials: X
authors.lastname: Wang
authors.forename: Hongsheng
authors.initials: H
authors.lastname: Bi
mesh_terms: D019264:Adoptive Transfer; D000328:Adult; D000818:Animals; D020256:Choroidal Neovascularization / Q000175:diagnosis; D004195:Disease Models, Animal; D019773:Epiretinal Membrane / Q000175:diagnosis; D005136:Eye Proteins; D005260:Female; D005451:Fluorescein Angiography; D006801:Humans; D008266:Macula Lutea / Q000473:pathology*; D008269:Macular Edema / Q000175:diagnosis; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008875:Middle Aged; D009887:Ophthalmoscopy; D012163:Retinal Detachment / Q000175:diagnosis; D012167:Retinal Perforations / Q000175:diagnosis; D012177:Retinol-Binding Proteins; D013601:T-Lymphocytes / Q000276:immunology; D041623:Tomography, Optical Coherence / Q000379:methods*; D014605:Uveitis / Q000175:diagnosis* / Q000276:immunology; D000099214:Retinol-Binding Proteins, Interstitial
chemical_list: D005136:Eye Proteins; D012177:Retinol-Binding Proteins; D000099214:Retinol-Binding Proteins, Interstitial