title: [O3:K6 serogroup vibrio parahaemolyticus - the causative agent of food toxic infection outbreaks in Primorsky region of Russian federation].
abstract: OBJECTIVE
  Comparative evaluation of biological properties of parahemolytic vibrios that had determined outbreaks and sporadic cases of food toxic infection in Primorsky Region in 2012 and previous years. Materials AND METHODS: 40 clinical strains of Vibrio parahaemolyticus isolated in 2012 were studied in comparison with 62 strains from this region that had been characterized by us previously. Virulence was evaluated by a complex method: hemolytic activitywas determined in Kanagawa test (KT), urease - in Kristensen medium. Serotyping was carried out by a commercial kit of O/K sera. PCR-genotyping was carried out by marker genes of 7 pathogenicity "islands" (VPaI-1-7).
  
  
  RESULTS
  All the strains isolated from patients in 2012 had KT-positive and urease-negative phenotype, belonged to O3:K6 serogroup and contained marker genes of 7 VPal that allowed to consider them members of a "pandemic" clone as the other clinical strains from this region. However among 2012 strains an increase of number of antibiotic-resistant variants was established compared with 1997 isolates.
  
  
  CONCLUSIONS
  The data obtained give evidence on the risk of spread of a "pandemic" clone of V. parahaemolyticus in the Far-Eastern region of Russia, a dangerous tendency of antibiotic-resistant variant formation and a necessity to monitor morbidity and the environment with mandatory PCR-detection of genes associated with virulence including integrated into pathogenicity "islands".
authors.forename: O A
authors.initials: OA
authors.lastname: Rykovskaia
authors.forename: A V
authors.initials: AV
authors.lastname: Mazrukho
authors.forename: L M
authors.initials: LM
authors.lastname: Smolikova
authors.forename: E V
authors.initials: EV
authors.lastname: Monakhova
authors.forename: O S
authors.initials: OS
authors.lastname: Chemisova
authors.forename: O A
authors.initials: OA
authors.lastname: Podoinitsyna
authors.forename: A V
authors.initials: AV
authors.lastname: Allenov
authors.forename: T V
authors.initials: TV
authors.lastname: Khomenko
authors.forename: A Ia
authors.initials: AIa
authors.lastname: Zhirov
authors.forename: E M
authors.initials: EM
authors.lastname: Sanamiants
authors.forename: N M
authors.initials: NM
authors.lastname: Gaevskaia
authors.forename: T A
authors.initials: TA
authors.lastname: Kudriakova
authors.forename: R R
authors.initials: RR
authors.lastname: Dalikova
authors.forename: M M
authors.initials: MM
authors.lastname: Sagakiants
mesh_terms: D004196:Disease Outbreaks; D006801:Humans; D016133:Polymerase Chain Reaction; D012426:Russia; D012703:Serotyping; D014735:Vibrio Infections / Q000453:epidemiology* / Q000382:microbiology; D014736:Vibrio parahaemolyticus / Q000302:isolation & purification / Q000472:pathogenicity*