title: Alleviation of Dimethylnitrosamine-Induced Liver Injury and Fibrosis by Supplementation of Anabasis articulata Extract in Rats.
abstract: Anabasis articulata (Forssk) Moq. (Chenopodiaceae) is an herb, grows in Egypt, and used in folk medicine to treat diabetes, fever, and kidney infections. The protective and therapeutic effects of the ethanol extract of A. articulata aerial parts were evaluated against dimethylnitrosamine (DMN)-induced liver fibrosis, compared with the standard drug, silymarin. Hepatic hydroxyproline content, serum transforming growth factor-β1 (TGF-β1), interleukin 10 (IL-10) and fructosamine were measured as liver fibrosis markers. Hepatic malondialdehyde (MDA), nitric oxide (NO), catalase (CAT), glutathione reductase (GR) and glutathione content (GSH) were measured as oxidant/antioxidant markers. Parallel histopathological investigations were also performed. Protective and therapeutic administration of A. articulata (100 mg/kg daily for 4 weeks), markedly prevented DMN-induced loss in body and liver weights. The extract significantly inhibited the elevation of hepatic hydroxyproline, NO and MDA (P < 0.05), as well as serum fructosamine, and TGF-β1 (P < 0.05) induced by DMN while it restored IL-10 to normal level in both protective and therapeutic groups. Furthermore, A. articulata prevented the depletion in CAT, GR, and GSH levels (P ≤ 0.05). In addition, oral administration of A. articulata extract and silymarin to both protective and therapeutic groups reduced the increase in liver function enzyme activities; alanine and aspartate amintransferases, gamma-glutamyl transferase in addition to alkaline phosphatase, and caused significant increase in serum albumin concentration as compared to DMN group. These data corresponded closely with those obtained for the drug silymarin. Histopathological studies confirmed the biochemical data and revealed remarkable improvement in liver architecture. Thus, it could be concluded that, A. articulata extract exhibited in vivo hepatoprotective and therapeutic effects against DMN-induced liver injury and may act as a useful agent in controlling the progression of hepatic fibrosis through reduction of oxidative stress and improving liver function.
authors.affiliation: Therapeutic Chemistry Department, National Research Centre, Cairo, Egypt.
authors.forename: Azza M
authors.initials: AM
authors.lastname: Mohamed
authors.affiliation: Faculty of Science, Helwan University, Cairo, Egypt.
authors.forename: Mohga S
authors.initials: MS
authors.lastname: Abdalla
authors.affiliation: Therapeutic Chemistry Department, National Research Centre, Cairo, Egypt.
authors.forename: Maha Z
authors.initials: MZ
authors.lastname: Rizk
authors.affiliation: Faculty of Science, Helwan University, Cairo, Egypt.
authors.forename: El-Sayed M E
authors.initials: el-SM
authors.lastname: Mahdy
authors.affiliation: Pathology Department, National Research Centre, Cairo, Egypt.
authors.forename: Abdel-Razik H
authors.initials: AR
authors.lastname: Farrag
authors.affiliation: Department of Chemistry of Natural and Microbial Products, National Research Centre, Cairo, Egypt.
authors.forename: Fatma S
authors.initials: FS
authors.lastname: El-Sharabasy
authors.affiliation: Therapeutic Chemistry Department, National Research Centre, Cairo, Egypt.
authors.forename: Hanan F
authors.initials: HF
authors.lastname: Aly
authors.affiliation: Therapeutic Chemistry Department, National Research Centre, Cairo, Egypt.
authors.forename: Mohamed R
authors.initials: MR
authors.lastname: Mohamed
keywords: Anabasis articulata; Dimethylnitrosamine; Liver fibrosis; Oxidative stress