title: Authenticity control of game meat products--a single method to detect and quantify adulteration of fallow deer (Dama dama), red deer (Cervus elaphus) and sika deer (Cervus nippon) by real-time PCR.
abstract: This contribution presents a single real-time PCR assay allowing the determination of the deer content (the sum of fallow deer (Dama dama), red deer (Cervus elaphus) and sika deer (Cervus nippon)) in meat products to detect food adulteration. The PCR assay does not show cross-reactivity with 20 animal species and 43 botanical species potentially contained in game meat products. The limit of quantification is 0.5% for fallow deer and red deer and 0.1% for sika deer. The deer content in meat products is determined by relating the concentration obtained with the deer PCR assay to that obtained with a reference system which amplifies mammals and poultry DNA. The analysis of binary meat mixtures with pork, a meat mixture containing equal amounts of fallow deer, red deer and sika deer in pork and a model game sausage showed that the quantification approach is very accurate (systematic error generally <25%).
authors.affiliation: Austrian Agency for Health and Food Safety, Institute for Food Safety, Department of Molecular Biology and Microbiology, Spargelfeldstraße 191, 1220 Vienna, Austria; Department of Analytical Chemistry, University of Vienna, Währinger Straße 38, 1090 Vienna, Austria.
authors.forename: Barbara
authors.initials: B
authors.lastname: Druml
authors.affiliation: Austrian Agency for Health and Food Safety, Institute for Food Safety, Department of Molecular Biology and Microbiology, Spargelfeldstraße 191, 1220 Vienna, Austria; Department of Analytical Chemistry, University of Vienna, Währinger Straße 38, 1090 Vienna, Austria.
authors.forename: Stephanie
authors.initials: S
authors.lastname: Grandits
authors.affiliation: Austrian Agency for Health and Food Safety, Institute for Food Safety, Department of Molecular Biology and Microbiology, Spargelfeldstraße 191, 1220 Vienna, Austria.
authors.forename: Walter
authors.initials: W
authors.lastname: Mayer
authors.affiliation: Austrian Agency for Health and Food Safety, Institute for Food Safety, Department of Molecular Biology and Microbiology, Spargelfeldstraße 191, 1220 Vienna, Austria.
authors.forename: Rupert
authors.initials: R
authors.lastname: Hochegger
authors.affiliation: Department of Analytical Chemistry, University of Vienna, Währinger Straße 38, 1090 Vienna, Austria. Electronic address: margit.cichna@univie.ac.at.
authors.forename: Margit
authors.initials: M
authors.lastname: Cichna-Markl
mesh_terms: D000818:Animals; D004247:DNA / Q000032:analysis; D003670:Deer / Q000235:genetics*; D005506:Food Contamination / Q000032:analysis*; D005524:Food Technology / Q000379:methods*; D008460:Meat / Q000032:analysis; D008461:Meat Products / Q000032:analysis*; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D004247:DNA
keywords: Adulteration; Deer; Game meat; Quantification; Real-time PCR