title: [Molecular and cellular aspects of dioxin toxicity].
abstract: BACKGROUND
  Using methods of molecular toxicology to study dioxin intoxication consequences the contribution was accessed of pathologic alterations induced and manifested by specific biomarkers and ecogenetic effects among Vietnamese population living on contaminated territories. The causes of variability in individual sensitivity to toxic activity were also evaluated.
  
  
  METHODS
  Individual biomedical indices were compared between those living in contaminated with dioxins (n = 8142) and control (n = 4421) regions. Dioxin concentrations were measured by high resolution chromato-mass spectrometry (84 samples). The characteristics of cytochrome P-450 system state (94 persons) and cytogenetic parameters (368 persons, 331 450 cells) reflected the molecular and genetic effects. Variable sensitivity to dioxins was demonstrated by associations of genetic polymorphism (CYP1A1, GSTM1, GSTT1, n = 195) and congenital morphogenetic variants among children (n = 1734).
  
  
  RESULTS
  Numerous consequences were demonstrated among the exposed individuals: noticeable absobtion of dioxins from environmental objects; direct effects of P-450 system's induction; systemic alterations in nucleus and genetic stability; changes in cellular generation's rate. The associations were revealed of genetic polymorphism in xenobiotic biotransformation/detoxification system and the peculiarities of development and morphogenesis among exposed children.
  
  
  CONCLUSIONS
  Characteristics of population chronicle intoxication with dioxins permitted to describe its numerous preclinical and clinical manifestations, to show the key elements in pathogenesis of revealed alterations. Future investigations are to create the groundwork for developing a method for prevention of dioxin pathology induction and realization based on revealing preclinical signs and effects of intoxication.
authors.forename: V S
authors.initials: VS
authors.lastname: Rumak
authors.forename: N V
authors.initials: NV
authors.lastname: Umnova
authors.forename: G A
authors.initials: GA
authors.lastname: Sofronov
mesh_terms: D000328:Adult; D001711:Biotransformation / Q000235:genetics; D002648:Child; D019363:Cytochrome P-450 CYP1A1 / Q000235:genetics / Q000378:metabolism; D004781:Environmental Exposure; D004782:Environmental Health; D005260:Female; D005819:Genetic Markers; D014644:Genetic Variation; D005982:Glutathione Transferase / Q000235:genetics / Q000378:metabolism; D006801:Humans; D008297:Male; D008875:Middle Aged; D000072317:Polychlorinated Dibenzodioxins / Q000493:pharmacokinetics / Q000633:toxicity; D015262:Xenobiotics / Q000493:pharmacokinetics / Q000633:toxicity
chemical_list: D019363:Cytochrome P-450 CYP1A1; D005819:Genetic Markers; D005982:Glutathione Transferase; D000072317:Polychlorinated Dibenzodioxins; D015262:Xenobiotics; C413545:glutathione S-transferase T1