title: The solution structure of the forkhead box-O DNA binding domain of Brugia malayi DAF-16a.
abstract: Brugia malayi is a parasitic nematode that causes lymphatic filariasis in humans. Here the solution structure of the forkhead DNA binding domain of Brugia malayi DAF-16a, a putative ortholog of Caenorhabditis elegans DAF-16, is reported. It is believed to be the first structure of a forkhead or winged helix domain from an invertebrate. C. elegans DAF-16 is involved in the insulin/IGF-I signaling pathway and helps control metabolism, longevity, and development. Conservation of sequence and structure with human FOXO proteins suggests that B. malayi DAF-16a is a member of the FOXO family of forkhead proteins.
authors.affiliation: Department of Biological Sciences, University of Wisconsin-Whitewater, Whitewater, Wisconsin, 53190; Department of Health Sciences, Blackhawk Technical College, Janesville, Wisconsin, 53545.
authors.forename: Sarah K
authors.initials: SK
authors.lastname: Casper
authors.forename: Scott J
authors.initials: SJ
authors.lastname: Schoeller
authors.forename: Danielle M
authors.initials: DM
authors.lastname: Zgoba
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: Phillips
authors.forename: Thomas J
authors.initials: TJ
authors.lastname: Morien
authors.forename: Gary R
authors.initials: GR
authors.lastname: Chaffee
authors.forename: Peter C
authors.initials: PC
authors.lastname: Sackett
authors.forename: Francis C
authors.initials: FC
authors.lastname: Peterson
authors.forename: Kirsten
authors.initials: K
authors.lastname: Crossgrove
authors.forename: Christopher T
authors.initials: CT
authors.lastname: Veldkamp
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D017178:Brugia malayi / Q000378:metabolism*; D029742:Caenorhabditis elegans Proteins / Q000737:chemistry / Q000378:metabolism; D017124:Conserved Sequence; D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D024202:Electrophoretic Mobility Shift Assay; D051858:Forkhead Transcription Factors / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D015801:Helminth Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D008958:Models, Molecular; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010446:Peptide Fragments / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D011487:Protein Conformation; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D020218:Response Elements; D016415:Sequence Alignment; D012995:Solubility
chemical_list: D029742:Caenorhabditis elegans Proteins; D051858:Forkhead Transcription Factors; D015801:Helminth Proteins; D010446:Peptide Fragments; D011994:Recombinant Proteins; C108831:daf-16 protein, C elegans; D004247:DNA
keywords: Brugia malayi; Caenorhabditis elegans; DAF-16; FOXO; FOXO1; FOXO3a; FOXO4; filariasis; forkhead box; insulin-signaling pathway; winged helix