title: Human umbilical cord mesenchymal stem cells infected with adenovirus expressing HGF promote regeneration of damaged neuron cells in a Parkinson's disease model.
abstract: Parkinson's disease (PD) is a neurodegenerative movement disorder that is characterized by the progressive degeneration of the dopaminergic (DA) pathway. Mesenchymal stem cells derived from human umbilical cord (hUC-MSCs) have great potential for developing a therapeutic agent as such. HGF is a multifunctional mediator originally identified in hepatocytes and has recently been reported to possess various neuroprotective properties. This study was designed to investigate the protective effect of hUC-MSCs infected by an adenovirus carrying the HGF gene on the PD cell model induced by MPP+ on human bone marrow neuroblastoma cells. Our results provide evidence that the cultural supernatant from hUC-MSCs expressing HGF could promote regeneration of damaged PD cells at higher efficacy than the supernatant from hUC-MSCs alone. And intracellular free Ca(2+) obviously decreased after treatment with cultural supernatant from hUC-MSCs expressing HGF, while the expression of CaBP-D28k, an intracellular calcium binding protein, increased. Therefore our study clearly demonstrated that cultural supernatant of MSC overexpressing HGF was capable of eliciting regeneration of damaged PD model cells. This effect was probably achieved through the regulation of intracellular Ca(2+) levels by modulating of CaBP-D28k expression.
authors.affiliation: Weifang Medical University, 7166 Baotong Road, Weifang 261053, China.
authors.forename: Xin-Shan
authors.initials: XS
authors.lastname: Liu
authors.affiliation: The Neurology Department, The 148th Hospital, 20 Zhanbei Road, Zibo 255300, China.
authors.forename: Jin-Feng
authors.initials: JF
authors.lastname: Li
authors.affiliation: Weifang Medical University, 7166 Baotong Road, Weifang 261053, China.
authors.forename: Shan-Shan
authors.initials: SS
authors.lastname: Wang
authors.affiliation: Medical College of Henan University, 357 Jinming Road, Kaifeng 475001, China.
authors.forename: Yu-Tong
authors.initials: YT
authors.lastname: Wang
authors.affiliation: The Neurology Department, The 148th Hospital, 20 Zhanbei Road, Zibo 255300, China.
authors.forename: Yu-Zhen
authors.initials: YZ
authors.lastname: Zhang
authors.affiliation: The Neurology Department, The 148th Hospital, 20 Zhanbei Road, Zibo 255300, China.
authors.forename: Hong-Lei
authors.initials: HL
authors.lastname: Yin
authors.affiliation: The Neurology Department, The 148th Hospital, 20 Zhanbei Road, Zibo 255300, China.
authors.forename: Shuang
authors.initials: S
authors.lastname: Geng
authors.affiliation: The Neurology Department, The 148th Hospital, 20 Zhanbei Road, Zibo 255300, China.
authors.forename: Hui-Cui
authors.initials: HC
authors.lastname: Gong
authors.affiliation: The Neurology Department, The 148th Hospital, 20 Zhanbei Road, Zibo 255300, China.
authors.forename: Bing
authors.initials: B
authors.lastname: Han
authors.affiliation: The Neurology Department, The 148th Hospital, 20 Zhanbei Road, Zibo 255300, China.
authors.forename: Yun-Liang
authors.initials: YL
authors.lastname: Wang
mesh_terms: D000256:Adenoviridae / Q000378:metabolism*; D064092:Calbindin 1 / Q000378:metabolism; D002118:Calcium / Q000378:metabolism; D002460:Cell Line; D002469:Cell Separation; D002470:Cell Survival; D017077:Culture Media, Conditioned / Q000494:pharmacology; D004797:Enzyme-Linked Immunosorbent Assay; D017228:Hepatocyte Growth Factor / Q000378:metabolism*; D006801:Humans; D042541:Intracellular Space / Q000378:metabolism; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism*; D008954:Models, Biological; D009416:Nerve Regeneration; D009474:Neurons; D010300:Parkinson Disease / Q000473:pathology*; D014161:Transduction, Genetic; D014470:Umbilical Cord / Q000166:cytology*
chemical_list: D064092:Calbindin 1; D017077:Culture Media, Conditioned; D017228:Hepatocyte Growth Factor; D002118:Calcium