title: Double-stranded DNA stereoselectively promotes aggregation of amyloid-like fibrils and generates peptide/DNA matrices.
abstract: An amyloidogenic LAM-L peptide (AASIKVAVSADR, all-L configuration) derived from laminin promoted cell adhesion, neurite outgrowth, and angiogenesis. Here, we prepared novel matrices using double-stranded DNA and the LAM-L peptide. Double-stranded DNA promoted aggregation of amyloid-like fibrils and generated a LAM-L/DNA matrix through electrostatic interactions between the phosphate groups of DNA and the amino groups of LAM-L. This formation of peptide/DNA matrix depends on the Ile-Lys-Val-Ala-Val (IKVAV) sequence in the peptide, since LAM-RM peptide (AASVVIAKSADR), which is scrambled peptide of LAM-L, did not form a matrix with DNA. Further, LAM-D (all-D configuration of LAM-L), which forms amyloid-like fibrils and promotes similar biological activities as LAM-L, did not form amyloid-like fibrils with DNA, suggesting that DNA selectively interacts with the L-configured peptide. Moreover, the LAM-L/DNA matrices showed stronger cell attachment activity compared with LAM-L alone, suggesting the LAM-L/DNA matrices have potential for use as a novel biomaterial in tissue engineering.
authors.affiliation: Department of Chemistry, Faculty of Science, Okayama University of Science, Ridaicho, Kita-ku, Okayama, 700-0005, Japan.
authors.forename: Masanori
authors.initials: M
authors.lastname: Yamada
authors.forename: Sachiko
authors.initials: S
authors.lastname: Hara
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Yamada
authors.forename: Fumihiko
authors.initials: F
authors.lastname: Katagiri
authors.forename: Kentaro
authors.initials: K
authors.lastname: Hozumi
authors.forename: Motoyoshi
authors.initials: M
authors.lastname: Nomizu
mesh_terms: D000595:Amino Acid Sequence; D000682:Amyloid / Q000737:chemistry*; D002448:Cell Adhesion; D045744:Cell Line, Tumor; D003224:Congo Red; D004247:DNA / Q000737:chemistry* / Q000648:ultrastructure; D004587:Electrophoresis, Agar Gel; D006801:Humans; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry*; D066329:Protein Aggregates; D013056:Spectrophotometry, Ultraviolet; D013194:Staining and Labeling; D013237:Stereoisomerism
chemical_list: D000682:Amyloid; D010455:Peptides; D066329:Protein Aggregates; D003224:Congo Red; D004247:DNA
keywords: DNA; cell adhesion; laminin; peptide; scaffold