title: N-alkylpyridinium quaternization combined with liquid chromatography-electrospray ionization-tandem mass spectrometry: A highly sensitive method to quantify fatty alcohols in thyroid tissues.
abstract: A highly sensitive method was developed for the identification and quantification of fatty alcohols in biological tissues. In the presence of pyridine-d0 and triflic anhydride (Tf2O), fatty alcohols were converted into permanently charged N-alkylpyridinium ions. Stable isotope-labeled derivatives were generated by pyridine-d5 and added as internal standard (IS). The mixture was analyzed by liquid chromatography coupled to positive electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS). This method was optimized and validated in terms of reaction time, derivatization efficiency, stability, desalting, and ion suppression effect. Besides, fatty alcohols exhibited good linear relationship (r(2)>0.993) over the concentration range of 10 ngmL(-1)-1 μgmL(-1). The limits of detection (LODs) were lowered from previously reported 0.1 ngmL(-1) to 0.25 pgmL(-1). Precision (RSD%<15.6%), accuracy (93.0-107.2%), matrix effect, and recovery (in thyroid tissues) were validated as well. Finally, this method was applied for the analysis of ten even carbon-numbered fatty alcohols (C8-C24) in human thyroid carcinoma and para-carcinoma tissues, revealing a significant decrease of fatty alcohols (free and esterified) in thyroid carcinoma tissues (p<0.05).
authors.affiliation: National Center for Organic Mass Spectrometry in Shanghai, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, 200032 Shanghai, China.
authors.forename: Yanjing
authors.initials: Y
authors.lastname: Cao
authors.affiliation: Department of Head and Neck Surgery, Fudan University Shanghai Cancer Center (FUSCC), 200032 Shanghai, China; Department of Oncology, Shanghai Medical College, Fudan University, 200032 Shanghai, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Guan
authors.affiliation: Department of Head and Neck Surgery, Fudan University Shanghai Cancer Center (FUSCC), 200032 Shanghai, China; Department of Oncology, Shanghai Medical College, Fudan University, 200032 Shanghai, China. Electronic address: tuanqisun@163.com.
authors.forename: Tuanqi
authors.initials: T
authors.lastname: Sun
authors.affiliation: National Center for Organic Mass Spectrometry in Shanghai, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, 200032 Shanghai, China.
authors.forename: Hang
authors.initials: H
authors.lastname: Wang
authors.affiliation: National Center for Organic Mass Spectrometry in Shanghai, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, 200032 Shanghai, China.
authors.forename: Jiapeng
authors.initials: J
authors.lastname: Leng
authors.affiliation: National Center for Organic Mass Spectrometry in Shanghai, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, 200032 Shanghai, China. Electronic address: ylguo@sioc.ac.cn.
authors.forename: Yinlong
authors.initials: Y
authors.lastname: Guo
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods*; D005233:Fatty Alcohols / Q000032:analysis*; D005260:Female; D006801:Humans; D057230:Limit of Detection; D008297:Male; D011726:Pyridinium Compounds / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods; D013961:Thyroid Gland / Q000737:chemistry*; D013964:Thyroid Neoplasms / Q000737:chemistry*
chemical_list: D005233:Fatty Alcohols; D011726:Pyridinium Compounds
keywords: Fatty alcohols; Isotope labeling; Multiple reaction monitoring; N-alkylpyridinium quaternization; Thyroid carcinoma