title: Silk fibroin nanoparticles prepared by electrospray as controlled release carriers of cisplatin.
abstract: To maintain the anti-tumor activity of cis-dichlorodiamminoplatinum (CDDP) while avoiding its cytotoxicity and negative influence on normal tissue, CDDP-loaded silk fibroin nanoparticles approximately 59 nm in diameter were successfully prepared by electrospray without using organic solvent. CDDP was incorporated into nanoparticles through metal-polymer coordination bond exchange. In vitro release tests showed that the cisplatin in the nanoparticles could be slowly and sustainably released for more than 15 days. In vitro anti-cancer experiments and intracellular Pt content testing indicated that CDDP-loaded silk fibroin nanoparticles were easily internalized by A549 lung cancer cells, transferring CDDP into cancer cells and then triggering their apoptosis. In contrast, the particles were not easily internalized by L929 mouse fibroblast cells and hence showed weaker cell growth inhibition. The CDDP-loaded silk fibroin nanoparticles showed sustained and efficient killing of tumor cells but weaker inhibition of normal cells. In general, this study provides not only a novel method for preparing CDDP-loaded silk fibroin nanoparticles but also a new carrier system for clinical therapeutic drugs against lung cancers and other tumors.
authors.affiliation: National Engineering Laboratory for Modern Silk, College of Textile and Clothing Engineering, Soochow University, No. 199 Ren''ai Road, Industrial Park, Suzhou 215123, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Qu
authors.affiliation: National Engineering Laboratory for Modern Silk, College of Textile and Clothing Engineering, Soochow University, No. 199 Ren''ai Road, Industrial Park, Suzhou 215123, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Liu
authors.affiliation: National Engineering Laboratory for Modern Silk, College of Textile and Clothing Engineering, Soochow University, No. 199 Ren''ai Road, Industrial Park, Suzhou 215123, China.
authors.forename: Yanni
authors.initials: Y
authors.lastname: Yu
authors.affiliation: National Engineering Laboratory for Modern Silk, College of Textile and Clothing Engineering, Soochow University, No. 199 Ren''ai Road, Industrial Park, Suzhou 215123, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.affiliation: National Engineering Laboratory for Modern Silk, College of Textile and Clothing Engineering, Soochow University, No. 199 Ren''ai Road, Industrial Park, Suzhou 215123, China.
authors.forename: Jingwan
authors.initials: J
authors.lastname: Luo
authors.affiliation: National Engineering Laboratory for Modern Silk, College of Textile and Clothing Engineering, Soochow University, No. 199 Ren''ai Road, Industrial Park, Suzhou 215123, China. Electronic address: mzli@suda.edu.cn.
authors.forename: Mingzhong
authors.initials: M
authors.lastname: Li
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D002945:Cisplatin / Q000494:pharmacology*; D003692:Delayed-Action Preparations / Q000494:pharmacology; D004337:Drug Carriers / Q000737:chemistry; D016503:Drug Delivery Systems / Q000379:methods*; D005349:Fibroins / Q000737:chemistry*; D006801:Humans; D051379:Mice; D053758:Nanoparticles / Q000737:chemistry; D018675:Toxicity Tests
chemical_list: D000970:Antineoplastic Agents; D003692:Delayed-Action Preparations; D004337:Drug Carriers; D005349:Fibroins; D002945:Cisplatin
keywords: Cisplatin; Electrospray; Nanoparticle; Silk fibroin