title: Preparation and application of sulfaguanidine-imprinted polymer on solid-phase extraction of pharmaceuticals from water.
abstract: The molecularly imprinted polymers (MIPs) with sulfaguanidine as a template, methacrylic acid, 4-vinylpyridine, and 2-hydroxyethyl methacrylate as functional monomers, ethylene glycol dimethacrylate as a cross-linker and 2,2'-azobis-isobutyronitrile as an initiator have been prepared through the cross-link reaction of polymerization. Solid-phase extraction (SPE) procedure for the extraction of sulfaguanidine from water samples using the prepared MIPs and non-imprinted (NIPs) was evaluated. The best MIP in combination with commercial sorbents was applied for simultaneous extraction of eight pharmaceuticals. New SPE cartridges were prepared by combination of optimal produced MIP and Oasis HLB in 6 mL of polypropilene SPE reservoir. The developed method which includes new SPE cartridge (MIPMAA-Oasis HLB, 400mg/6 mL) and thin-layer chromatography was validated. The method provides a linear response over the concentration range of 0.5-150 μg/L, depending on the pharmaceutical with the correlation coefficients>0.9843 in all cases except for norfloxacin (0.9770) and penicillin G procaine (0.9801). Also, the method has revealed low limits of detection (0.25-20 μg/L), good precision (intra and inter-day), a relative standard deviation below 15% and recoveries above 95% for all eight pharmaceuticals. The developed method by using newly prepared SPE cartridge has been successfully applied to the analysis of production wastewater samples from pharmaceutical industry.
authors.affiliation: Department of Analytical Chemistry, Faculty of Chemical Engineering and Technology, University of Zagreb, Marulićev trg 19, 10000 Zagreb, Croatia. Electronic address: dmutavdz@fkit.hr.
authors.forename: Dragana
authors.initials: D
authors.lastname: Mutavdžić Pavlović
authors.affiliation: Department of Analytical Chemistry, Faculty of Chemical Engineering and Technology, University of Zagreb, Marulićev trg 19, 10000 Zagreb, Croatia.
authors.forename: Korana
authors.initials: K
authors.lastname: Nikšić
authors.affiliation: Department of Analytical Chemistry, Faculty of Chemical Engineering and Technology, University of Zagreb, Marulićev trg 19, 10000 Zagreb, Croatia.
authors.forename: Sara
authors.initials: S
authors.lastname: Livazović
authors.affiliation: Faculty of Metallurgy, University of Zagreb, Aleja narodnih heroja 3, 44103 Sisak, Croatia.
authors.forename: Ivan
authors.initials: I
authors.lastname: Brnardić
authors.affiliation: National Institute of Chemistry, Hajdrihova 19, SI-1000 Ljubljana, Slovenia.
authors.forename: Alojz
authors.initials: A
authors.lastname: Anžlovar
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002855:Chromatography, Thin Layer / Q000379:methods; D008855:Microscopy, Electron, Scanning; D054802:Molecular Imprinting; D004364:Pharmaceutical Preparations / Q000302:isolation & purification*; D011108:Polymers / Q000737:chemistry*; D012722:Sewage / Q000032:analysis; D052616:Solid Phase Extraction / Q000379:methods*; D017550:Spectroscopy, Fourier Transform Infrared; D013414:Sulfaguanidine / Q000737:chemistry*; D014874:Water Pollutants, Chemical / Q000302:isolation & purification*
chemical_list: D004364:Pharmaceutical Preparations; D011108:Polymers; D012722:Sewage; D014874:Water Pollutants, Chemical; D013414:Sulfaguanidine
keywords: Molecularly imprinted polymers; Pharmaceuticals; Production wastewater; Sulfaguanidine