title: Reduced uterine receptivity for mouse embryos developed from in-vitro matured oocytes.
abstract: OBJECTIVE
  The outcomes of in-vitro maturation (IVM) are inferior compared to those of IVF. The purpose of the study was to compare the implantation rates of IVM- and in-vivo maturation (IVO)- derived embryos, and to evaluate their effects on uterine receptivity.
  
  
  METHODS
  The IVM- and IVO- oocytes were obtained from female mice, fertilized and transferred to separate oviducts of the same pseudo-pregnant mice. After 5 days, the implanted blastocysts were dissected out of the uterine horns, and the uterine horns were analyzed for the expression of mRNAs encoding leukemia inhibitory factor, heparin-binding epidermal growth factor, insulin-like growth factor binding protein-4, progesterone receptor, and Hoxa-10.
  
  
  RESULTS
  The maturation rate of the IVM- oocytes was 81.2%. The fertilization rate of the IVM oocytes was lower than that of the IVO oocytes (50.5% vs. 78.0%, p = 0.038), as was their implantation rate (14.5% vs. 74.7%, p < 0.001). All 5 mRNAs examined were expressed at significantly lower levels in the uterine horns that received the IVM-derived embryos than in those that received the IVO-derived embryos.
  
  
  CONCLUSIONS
  The IVM-derived embryos are less competent in inducing expression of implantation-related mRNAs in the uterine horn.
authors.affiliation: Department of Obstetrics and Gynecology, Shin Kong Wu Ho-Su Memorial Hospital, No. 95, Wen Chang Road, Shih Lin District, Taipei, Taiwan.
authors.forename: Yu-Hung
authors.initials: YH
authors.lastname: Lin
authors.forename: Ching-Yen
authors.initials: CY
authors.lastname: Tsai
authors.forename: Lee-Wen
authors.initials: LW
authors.lastname: Huang
authors.forename: Kok-Min
authors.initials: KM
authors.lastname: Seow
authors.forename: Jiann-Loung
authors.initials: JL
authors.lastname: Hwang
authors.forename: Chii-Ruey
authors.initials: CR
authors.lastname: Tzeng
mesh_terms: D000818:Animals; D046149:Embryo Culture Techniques; D004624:Embryo Transfer; D005260:Female; D005307:Fertilization in Vitro; D018507:Gene Expression Regulation, Developmental; D066257:Heparin-binding EGF-like Growth Factor / Q000096:biosynthesis; D018398:Homeodomain Proteins / Q000096:biosynthesis; D006801:Humans; D059471:In Vitro Oocyte Maturation Techniques; D018970:Insulin-Like Growth Factor Binding Protein 1 / Q000096:biosynthesis; D051379:Mice; D009865:Oocytes / Q000254:growth & development* / Q000473:pathology; D011247:Pregnancy; D012333:RNA, Messenger / Q000096:biosynthesis; D011980:Receptors, Progesterone / Q000096:biosynthesis; D014599:Uterus / Q000378:metabolism / Q000503:physiopathology*
chemical_list: C587884:Hbegf protein, mouse; D066257:Heparin-binding EGF-like Growth Factor; D018398:Homeodomain Proteins; C094041:Hoxa11 protein, mouse; C519663:IGFBPL-1 protein, mouse; D018970:Insulin-Like Growth Factor Binding Protein 1; D012333:RNA, Messenger; D011980:Receptors, Progesterone