title: Wide-scale pollen banking of ornamental plants through cryopreservation.
abstract: BACKGROUND
  Cryopreservation has been proved to be an efficient method for the long-term storage of pollen, based on our previous studies establishing cryo pollen banks for Camellia, Paeonia, and Prunus mume.
  
  
  OBJECTIVE
  In the present study, we aimed to extend and verify the applicability of the cryopreservation protocol for ornamental plants.
  
  
  METHODS
  71 species or cultivars from 19 families were cryopreserved using the established protocol.
  
  
  RESULTS
  Pollen from 60 species or cultivars (85% of those experimented on) retained viability after 1 year's cryopreservation; of these, 36 (51% of the total investigated) retained a sufficiently high germination capability compared with fresh pollen to enable longer term cryopreservation.
  
  
  CONCLUSIONS
  This study confirms that wide-scale pollen banking of ornamental plants is feasible.
authors.affiliation: Beijing Key Laboratory of Ornamental Plants Germplasm Innovation and Molecular Breeding, National Engineering Research Center for Floriculture, College of Landscape Architecture, Beijing Forestry University, Beijing, China.
authors.forename: J
authors.initials: J
authors.lastname: Xu
authors.affiliation: Beijing Key Laboratory of Ornamental Plants Germplasm Innovation and Molecular Breeding, National Engineering Research Center for Floriculture, College of Landscape Architecture, Beijing Forestry University, Beijing, China.
authors.forename: B
authors.initials: B
authors.lastname: Li
authors.affiliation: Beijing Key Laboratory of Ornamental Plants Germplasm Innovation and Molecular Breeding, National Engineering Research Center for Floriculture, College of Landscape Architecture, Beijing Forestry University, Beijing, China.
authors.forename: Q
authors.initials: Q
authors.lastname: Liu
authors.affiliation: Beijing Key Laboratory of Ornamental Plants Germplasm Innovation and Molecular Breeding, National Engineering Research Center for Floriculture, College of Landscape Architecture, Beijing Forestry University, Beijing, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Shi
authors.affiliation: Beijing Key Laboratory of Ornamental Plants Germplasm Innovation and Molecular Breeding, National Engineering Research Center for Floriculture, College of Landscape Architecture, Beijing Forestry University, Beijing, China.
authors.forename: J
authors.initials: J
authors.lastname: Peng
authors.affiliation: Beijing Key Laboratory of Ornamental Plants Germplasm Innovation and Molecular Breeding, National Engineering Research Center for Floriculture, College of Landscape Architecture, Beijing Forestry University, Beijing, China.
authors.forename: M
authors.initials: M
authors.lastname: Jia
authors.affiliation: Beijing Key Laboratory of Ornamental Plants Germplasm Innovation and Molecular Breeding, National Engineering Research Center for Floriculture, College of Landscape Architecture, Beijing Forestry University, Beijing, China. chblyan@163.com.
authors.forename: Yan
authors.initials: Y
authors.lastname: Liu
mesh_terms: D018070:Biological Specimen Banks / Q000706:statistics & numerical data*; D002470:Cell Survival / Q000502:physiology; D015925:Cryopreservation / Q000379:methods*; D018525:Germination / Q000502:physiology; D019684:Magnoliopsida / Q000502:physiology*; D011058:Pollen / Q000502:physiology*