title: Anti-Mullerian hormone concentration and antral ovarian follicle population in Murrah heifers compared to Holstein and Gyr kept under the same management.
abstract: This study was performed to evaluate plasma concentrations of anti-Mullerian hormone (AMH) and the ovarian antral follicle population (AFP) in different genetic groups. Cyclic heifers (13 Bubalus bubalis [Murrah]; 15 Bos taurus [Holstein] and 10 Bos indicus [Gyr]) were maintained under the same management and were synchronized with two doses of 150 μg IM d-cloprostenol administered 14 days apart. After the second d-cloprostenol treatment, heifers had their ovaries scanned daily by ultrasound to define the day of ovulation. On the same day, the AFP was determined and a plasma sample was collected to measure AMH. Murrah heifers had less AFP (25.6 ± 2.1 follicles; p = 0.01) and plasma AMH concentration (0.18 ± 0.03 ng/ml; p < 0.001) than Gyr (60.0 ± 12.2 follicles and 0.60 ± 0.12 ng/ml of AMH); however, data were similar when compared to Holstein (35.9 ± 6.8 follicles and 0.24 ± 0.06 ng/ml of AMH) heifers. Regardless of genetic background, there was a positive relationship between the AFP and plasmatic AMH concentration (Murrah [r = 0.62; p < 0.01], Holstein [r = 0.66; p < 0.001] and Gyr [r = 0.88; p < 0.001]). Also, when heifers were classified according to high- or low-AMH concentration based on the average within each genetic group, high-AMH heifers had greater (p < 0.0001) AFP than low-AMH heifers. In conclusion, both Murrah and Holstein heifers presented lower plasma AMH concentration and AFP when compared to Gyr.
authors.affiliation: Department of Animal Reproduction, College of Animal Science and Veterinary Medicine, University of São Paulo, São Paulo, SP, Brazil.
authors.forename: J m
authors.initials: Jm
authors.lastname: Baldrighi
authors.forename: M F
authors.initials: MF
authors.lastname: Sá Filho
authors.forename: E O S
authors.initials: EO
authors.lastname: Batista
authors.forename: R N V R
authors.initials: RN
authors.lastname: Lopes
authors.forename: J A
authors.initials: JA
authors.lastname: Visintin
authors.forename: P S
authors.initials: PS
authors.lastname: Baruselli
authors.forename: M E O A
authors.initials: ME
authors.lastname: Assumpção
mesh_terms: D000822:Animal Husbandry; D000818:Animals; D054304:Anti-Mullerian Hormone / Q000378:metabolism*; D002020:Buffaloes / Q000097:blood / Q000378:metabolism*; D002417:Cattle / Q000097:blood / Q000378:metabolism*; D003008:Cloprostenol / Q000494:pharmacology*; D004973:Estrus Synchronization / Q000379:methods; D005260:Female; D006080:Ovarian Follicle / Q000187:drug effects* / Q000502:physiology; D013045:Species Specificity
chemical_list: D003008:Cloprostenol; D054304:Anti-Mullerian Hormone