title: Immunoisolated transplantation of purified langerhans islet cells in testis cortex of male rats for treatment of streptozotocin induced diabetes mellitus.
abstract: The objective of this study is to induce experimental diabetes mellitus by streptozotocin in normal adult Wistar rats via comparison of changes in body weight, consumption of food, volume of water, urine and levels of glucose, insulin and C-peptide in serum, between normal and diabetic rats. Intra-venous injection of 60 mg/kg dose of streptozotocin in 250-300 g (75-90 days) adult Wistar rats makes pancreas swell and causes degeneration in Langerhans islet β-cells and induces experimental diabetes mellitus in 2-4 days. For a microscopic study of degeneration of Langerhans islet β-cells of diabetic rats, biopsy from pancreas tissue of diabetic and normal rats, staining and comparison between them, were done. In this process, after collagenase digestion of pancreas, islets were isolated, dissociated and identified by dithizone method and then with enzymatic procedure by DNase and trypsin, the islet cells changed into single cells and β-cells were identified by immune fluorescence method and then assayed by flow-cytometer. Donor tissue in each step of work was prepared from 38 adult male Wistar rats weighted 250-300 g (75-90 days). Transplantation was performed in rats after 2-4 weeks of diabetes induction. In this study, the levels of insulin, C-peptide and glucose in diabetic rats reached to normal range as compared to un-diabetic rats in 20 days after transplantation of islet cells. Transplantation was performed under the cortex of testis as immunoisolated place for islet cells transplantation.
authors.affiliation: Pilot Biotechnology Department, Pasteur Institute of Iran, No. 69, 12 Farvardin Street, Jomhoori Avenue, 13169-43551 Tehran, Iran.
authors.forename: Ali
authors.initials: A
authors.lastname: Farhangi
authors.affiliation: Pilot Biotechnology Department, Pasteur Institute of Iran, No. 69, 12 Farvardin Street, Jomhoori Avenue, 13169-43551 Tehran, Iran.
authors.forename: Dariush
authors.initials: D
authors.lastname: Norouzian
authors.affiliation: Pilot Biotechnology Department, Pasteur Institute of Iran, No. 69, 12 Farvardin Street, Jomhoori Avenue, 13169-43551 Tehran, Iran.
authors.forename: Mohammad Reza
authors.initials: MR
authors.lastname: Mehrabi
authors.affiliation: Pilot Biotechnology Department, Pasteur Institute of Iran, No. 69, 12 Farvardin Street, Jomhoori Avenue, 13169-43551 Tehran, Iran.
authors.forename: Mohsen
authors.initials: M
authors.lastname: Chiani
authors.affiliation: Pilot Biotechnology Department, Pasteur Institute of Iran, No. 69, 12 Farvardin Street, Jomhoori Avenue, 13169-43551 Tehran, Iran.
authors.forename: Zahra
authors.initials: Z
authors.lastname: Saffari
authors.affiliation: Pilot Biotechnology Department, Pasteur Institute of Iran, No. 69, 12 Farvardin Street, Jomhoori Avenue, 13169-43551 Tehran, Iran.
authors.forename: Maryam
authors.initials: M
authors.lastname: Farahnak
authors.affiliation: Pilot Biotechnology Department, Pasteur Institute of Iran, No. 69, 12 Farvardin Street, Jomhoori Avenue, 13169-43551 Tehran, Iran.
authors.forename: Azim
authors.initials: A
authors.lastname: Akbarzadeh
keywords: Diabetes induction; Flow cytometry; Immunoisolated-transplantation; Islet cells; Purification; Streptozotocin