title: Variability in antioxidant/detoxification enzymes of Labeo rohita exposed to an azo dye, acid black (AB).
abstract: The aim of the present study was to evaluate effect of a highly toxic azo dye, acid black (AB) (CI: 20470, 96 h LC50=10 mg/L) on the biochemical responses of Labeo rohita. Antioxidant/detoxification enzymes such as glutathione-S-transferase (GST), glutathione reductase (GR), glutathione peroxidase (GPx), catalase (CAT), superoxide dismutase (SOD), acetylcholinesterase (AChE), lactate dehydrogenase (LDH), succinate dehydrogenase (SDH), acid phosphatase (AcP), alkaline phosphatase (AKP), alanine transaminase (ALT) and aspartate transaminase (AST) were determined in liver, kidney, gill, muscle and brain of L. rohita after 96 h exposure to 6 mg/L (LC10), 8 mg/L (LC30) and 10 mg/L (LC50) of dye with an aim to find out the target tissue and biomarker enzyme for AB. The fish were then kept for a recovery period of 90 days, and activity of the selected enzymes was determined at the end of this period. Present dye altered the activities of all these enzymes in the selected tissues of the experimental fish in a dose-dependent manner. SOD was the maximally affected enzyme, and liver was the most affected tissue. The results indicate that AB is very toxic to L. rohita as there was a significant effect of even 6 mg/L dose of the dye and the toxicity prolonged for a long time because the fish was not able to recover from the stress even 90 days after the exposure. The study suggests that SOD can be used as a biomarker enzyme and liver is the target tissue for AB.
authors.affiliation: Department of Zoology, Guru Nanak Dev University, Amritsar, Punjab 143005, India.
authors.forename: Satinder
authors.initials: S
authors.lastname: Kaur
authors.affiliation: Department of Zoology, Guru Nanak Dev University, Amritsar, Punjab 143005, India. Electronic address: arvinder99@yahoo.co.in.
authors.forename: Arvinder
authors.initials: A
authors.lastname: Kaur
mesh_terms: D000580:Amido Black / Q000008:administration & dosage / Q000633:toxicity*; D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D001391:Azo Compounds / Q000008:administration & dosage / Q000633:toxicity*; D001921:Brain / Q000187:drug effects / Q000201:enzymology; D002347:Carps; D004305:Dose-Response Relationship, Drug; D005880:Gills / Q000187:drug effects / Q000201:enzymology; D007668:Kidney / Q000187:drug effects / Q000201:enzymology; D008099:Liver / Q000187:drug effects / Q000201:enzymology; D018482:Muscle, Skeletal / Q000187:drug effects / Q000201:enzymology; D009282:Naphthalenesulfonates / Q000008:administration & dosage / Q000633:toxicity*; D018384:Oxidative Stress / Q000187:drug effects; D014874:Water Pollutants, Chemical / Q000008:administration & dosage / Q000633:toxicity
chemical_list: D000975:Antioxidants; D001391:Azo Compounds; D009282:Naphthalenesulfonates; D014874:Water Pollutants, Chemical; D000580:Amido Black
keywords: 96h LC(50); Acetylcholinesterase; Acid black; Antioxidant enzymes; Azo dye; Biomarker; Glutathione-S-transferase; Labeo rohita