title: The human mitotic kinesin KIF18A binds protein phosphatase 1 (PP1) through a highly conserved docking motif.
abstract: Protein phosphatase 1 (PP1), a serine/threonine protein phosphatase, controls diverse key cellular events. PP1 catalytic subunits form complexes with a variety of interacting proteins that control its ability to dephosphorylate substrates. Here we show that the human mitotic kinesin-8, KIF18A, directly interacts with PP1γ through a conserved RVxF motif. Our phylogenetic analyses of the kinesins further uncovered the broad conservation of this interaction potential within the otherwise highly diverse motor-protein superfamily. This suggests an ancestral origin of PP1 recruitment to KIF18A and a strategic role in human mitotic cells.
authors.affiliation: Department of Biological Sciences, University of Calgary, 2500 University Dr., Calgary, Alberta T2N 1N4, Canada.
authors.forename: Veerle
authors.initials: V
authors.lastname: De Wever
authors.affiliation: Department of Biological Sciences, University of Calgary, 2500 University Dr., Calgary, Alberta T2N 1N4, Canada.
authors.forename: Isha
authors.initials: I
authors.lastname: Nasa
authors.affiliation: Department of Cellular & Molecular Biology and Ottawa Institute of Systems Biology, University of Ottawa, Ottawa, ON, Canada.
authors.forename: Delphine
authors.initials: D
authors.lastname: Chamousset
authors.affiliation: Department of Biological Sciences, University of Calgary, 2500 University Dr., Calgary, Alberta T2N 1N4, Canada.
authors.forename: David
authors.initials: D
authors.lastname: Lloyd
authors.affiliation: Department of Biological Sciences, University of Calgary, 2500 University Dr., Calgary, Alberta T2N 1N4, Canada.
authors.forename: Mhairi
authors.initials: M
authors.lastname: Nimick
authors.affiliation: Department of Biological Sciences, University of Calgary, 2500 University Dr., Calgary, Alberta T2N 1N4, Canada.
authors.forename: Hui
authors.initials: H
authors.lastname: Xu
authors.affiliation: Department of Cellular & Molecular Biology and Ottawa Institute of Systems Biology, University of Ottawa, Ottawa, ON, Canada. Electronic address: ltrinkle@uottawa.ca.
authors.forename: Laura
authors.initials: L
authors.lastname: Trinkle-Mulcahy
authors.affiliation: Department of Biological Sciences, University of Calgary, 2500 University Dr., Calgary, Alberta T2N 1N4, Canada. Electronic address: moorhead@ucalgary.ca.
authors.forename: Greg B G
authors.initials: GB
authors.lastname: Moorhead
mesh_terms: D001665:Binding Sites; D006367:HeLa Cells; D006801:Humans; D016547:Kinesins / Q000378:metabolism*; D008938:Mitosis; D010802:Phylogeny; D054645:Protein Phosphatase 1 / Q000378:metabolism*
chemical_list: D054645:Protein Phosphatase 1; C502735:KIF18A protein, human; D016547:Kinesins
keywords: KIF18A; Kinesin; Klp5/6; Mitosis; Molecular evolution; Protein phosphatase 1