title: Ameliorative effect of Phytocee™ Cool against carbon tetrachloride-induced oxidative stress.
abstract: BACKGROUND
  Antioxidants from natural sources have a major role in reversing the effects of oxidative stress and promoting health, growth and productivity in animals.
  
  
  OBJECTIVE
  This study was undertaken to investigate the possible antioxidant activity and hepatoprotective effects of Phytocee™ Cool on carbon tetrachloride (CCl4) induced oxidative stress and liver damage in rats.
  
  
  METHODS
  Animals were pretreated with Phytocee™ Cool for 10 days and were challenged with CCl4 (1:1 v/v) in olive oil on the 10(th) day. After 24 h of CCl4 administration blood was collected and markers of hepatocellular damage aspartate aminotransferase (AST), alanine aminotransferase (ALT) were evaluated. Rats were sacrificed and oxidative stress in liver was estimated using malondialdehyde (MDA), reduced glutathione (GSH), superoxide dismutase (SOD) and catalase.
  
  
  RESULTS
  CCl4 caused a significant increase in serum AST, ALT, hepatic MDA and GSH levels, whereas the SOD and catalase activities were decreased. Phytocee™ Cool pretreatment attenuated the MDA, AST ALT levels and increased the activities of SOD and catalase.
  
  
  CONCLUSIONS
  Phytocee™ Cool demonstrated antioxidant potential and hepatoprotective effects and plausibly be used in the amelioration of oxidative stress.
authors.affiliation: Department of Veterinary Pharmacology and Toxicology, College of Veterinary and Animal Sciences, Mannuthy, Thrissur, Kerala, India.
authors.forename: Joshua Allan
authors.initials: JA
authors.lastname: Joseph
authors.affiliation: Department of Veterinary Pharmacology and Toxicology, College of Veterinary and Animal Sciences, Mannuthy, Thrissur, Kerala, India.
authors.forename: Usha Parackal Thachappully
authors.initials: UP
authors.lastname: Ayyappan
authors.affiliation: Pookode, Wayanad, Kerala, India.
authors.forename: Suja Rani
authors.initials: SR
authors.lastname: Sasidharan
authors.affiliation: Department of Pharmacology and Toxicology, R&D Centre, Natural Remedies, Bangalore, Karnataka, India.
authors.forename: Sridhar
authors.initials: S
authors.lastname: Mutyala
authors.affiliation: Department of Pharmacology and Toxicology, R&D Centre, Natural Remedies, Bangalore, Karnataka, India.
authors.forename: Krishnagouda Shankargouda
authors.initials: KS
authors.lastname: Goudar
authors.affiliation: Department of Pharmacology and Toxicology, R&D Centre, Natural Remedies, Bangalore, Karnataka, India.
authors.forename: Amit
authors.initials: A
authors.lastname: Agarwal
keywords: Antioxidant; Phytocee™ Cool; carbon tetrachloride; oxidative stress