title: Involvement of the yciW gene in l-cysteine and l-methionine metabolism in Escherichia coli.
abstract: We here analyzed a sulfur index of Escherichia coli using LC-MS/MS combined with thiol-specific derivatization by monobromobimane. The obtained sulfur index was then applied to evaluate the L-cysteine producer. E. coli cells overexpressing the yciW gene, a novel Cys regulon, accumulated l-homocysteine, suggesting that YciW is involved in L-methionine biosynthesis.
authors.affiliation: Graduate School of Biological Sciences, Nara Institute of Science and Technology, 8916-5 Takayama-cho, Ikoma, Nara 630-0192, Japan.
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Kawano
authors.affiliation: Graduate School of Biological Sciences, Nara Institute of Science and Technology, 8916-5 Takayama-cho, Ikoma, Nara 630-0192, Japan. Electronic address: iohtsu@bs.naist.jp.
authors.forename: Iwao
authors.initials: I
authors.lastname: Ohtsu
authors.affiliation: Graduate School of Biological Sciences, Nara Institute of Science and Technology, 8916-5 Takayama-cho, Ikoma, Nara 630-0192, Japan.
authors.forename: Ai
authors.initials: A
authors.lastname: Tamakoshi
authors.affiliation: Graduate School of Biological Sciences, Nara Institute of Science and Technology, 8916-5 Takayama-cho, Ikoma, Nara 630-0192, Japan.
authors.forename: Maeka
authors.initials: M
authors.lastname: Shiroyama
authors.affiliation: Graduate School of Biological Sciences, Nara Institute of Science and Technology, 8916-5 Takayama-cho, Ikoma, Nara 630-0192, Japan.
authors.forename: Ai
authors.initials: A
authors.lastname: Tsuruoka
authors.affiliation: Graduate School of Biological Sciences, Nara Institute of Science and Technology, 8916-5 Takayama-cho, Ikoma, Nara 630-0192, Japan.
authors.forename: Kyohei
authors.initials: K
authors.lastname: Saiki
authors.affiliation: Research Institute for Bioscience Products and Fine Chemicals, Ajinomoto Co., 1-1 Suzuki-cho, Kawasaki, Kanagawa 210-8681, Japan.
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Takumi
authors.affiliation: Research Institute for Bioscience Products and Fine Chemicals, Ajinomoto Co., 1-1 Suzuki-cho, Kawasaki, Kanagawa 210-8681, Japan.
authors.forename: Gen
authors.initials: G
authors.lastname: Nonaka
authors.affiliation: MS Business Unit, Shimadzu Co., 1 Nishinokyo, Kuwabara-cho, Nakagyo-ku, Kyoto 604-8511, Japan.
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Nakanishi
authors.affiliation: Department of Biochemistry, Keio University School of Medicine, 35 Shinanomachi, Shinjyuku-ku, Tokyo 160-8582, Japan.
authors.forename: Takako
authors.initials: T
authors.lastname: Hishiki
authors.affiliation: Department of Biochemistry, Keio University School of Medicine, 35 Shinanomachi, Shinjyuku-ku, Tokyo 160-8582, Japan.
authors.forename: Makoto
authors.initials: M
authors.lastname: Suematsu
authors.affiliation: Graduate School of Biological Sciences, Nara Institute of Science and Technology, 8916-5 Takayama-cho, Ikoma, Nara 630-0192, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Takagi
mesh_terms: D001643:Bridged Bicyclo Compounds / Q000378:metabolism; D003545:Cysteine / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism*; D006710:Homocysteine / Q000378:metabolism; D008715:Methionine / Q000378:metabolism*; D018085:Regulon / Q000235:genetics; D013455:Sulfur / Q000378:metabolism; D053719:Tandem Mass Spectrometry
chemical_list: D001643:Bridged Bicyclo Compounds; D006710:Homocysteine; D013455:Sulfur; D008715:Methionine; D003545:Cysteine; C028381:monobromobimane
keywords: Escherichia coli; Metabolome analysis; Monobromobimane; l-Cysteine; yciW gene