title: A glucose biosensor based on the synergistic action of nanometer-sized TiO2 and polyaniline.
abstract: Polyaniline/active carbon (PANI) and nanometer-sized TiO2 (n-TiO2) were prepared by oxidation and sol-gel methods, respectively, and were then used as a zymophore to modify a glassy carbon electrode (GCE) and a GOx/n-TiO2/PANI/GCE sensor with a synergistic effect was established. A series of performance evaluations for the modified material and sensor was studied in detail through cyclic voltammetry (CV) and a chronoamperometry (CA) method. The results showed that the sensor had a good response to glucose and that the electron of the GOx molecule was transferred directly onto the sensor, and a linear relationship between the GOx redox peak current and the sweep speed was found. The apparent transmission speed constant, k, for dissimilar electrode charges was 1.35s(-1), 95% of the maximum steady current for the GOx/n-TiO2/PANI/GCE sensor could be reached in 10s, the linear range of the detected glucose concentration was from 0.02 mM to 6.0mM, the sensitivity was 6.31 μA mM(-1)cm(-2), and the limit of detection was 18 μM. The sensor had good selectivity and stability and could be maintained at 82% of the initial activity for 30 days.
authors.affiliation: Department of Chemistry, Tongji University, Shanghai 200092, China. Electronic address: tangww@tongji.edu.cn.
authors.forename: Wenwei
authors.initials: W
authors.lastname: Tang
authors.affiliation: School of Life Science and Technology, Tongji University, Shanghai 200092, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Li
authors.affiliation: School of Life Science and Technology, Tongji University, Shanghai 200092, China. Electronic address: zengxp@tongji.edu.cn.
authors.forename: Xinping
authors.initials: X
authors.lastname: Zeng
mesh_terms: D000814:Aniline Compounds / Q000737:chemistry*; D015374:Biosensing Techniques / Q000379:methods*; D002244:Carbon / Q000737:chemistry*; D055664:Electrochemical Techniques / Q000379:methods; D004566:Electrodes; D004800:Enzymes, Immobilized / Q000737:chemistry; D005947:Glucose / Q000032:analysis*; D005949:Glucose Oxidase / Q000378:metabolism; D053768:Metal Nanoparticles / Q000737:chemistry*; D010084:Oxidation-Reduction; D014025:Titanium / Q000737:chemistry*
chemical_list: D000814:Aniline Compounds; D004800:Enzymes, Immobilized; C416807:polyaniline; C009495:titanium dioxide; D002244:Carbon; D014025:Titanium; D005949:Glucose Oxidase; D005947:Glucose
keywords: Amperometric glucose sensor; Detection limit; Direct electron transfer; Linear range; Polyaniline; n-TiO(2)