title: Non-invasive characterization of the adipogenic differentiation of human bone marrow-derived mesenchymal stromal cells by HS-SPME/GC-MS.
abstract: A non-invasive method to characterize human mesenchymal stromal cells during adipogenic differentiation was developed for the first time. Seven fatty acid methyl esters (FAMEs), including methyl laurate, methyl myristate, methyl palmitate, methyl linoleate, methyl oleate, methyl elaidate and methyl stearate, were used for characterizing adipogenic differentiation using headspace solid-phase microextraction (HS-SPME) which is a very simple and non-invasive method for the extraction of volatile compounds. Glassware was used for culturing mesenchymal stromal cells rather than the common plasticware to minimize contamination by volatile impurities. The optimal SPME fiber was selected by comparing diverse fibers containing two pure liquid polymers (PDMS and PA) and two porous solids (PDMS/DVB and CAR/PDMS). Using optimized procedures, we discovered that seven FAMEs were only detected in adipogenic differentiated mesenchymal stromal cells and not in the mesenchymal stromal cells before differentiation. These data could support the quality control of clinical mesenchymal stromal cell culture in the pharmaceutical industry in addition to the development of many clinical applications using mesenchymal stromal cells.
authors.affiliation: 1] College of Pharmacy and Research Institute of Pharmaceutical Sciences, Seoul National University, Seoul 151-742, Republic of Korea [2].
authors.forename: Dong-Kyu
authors.initials: DK
authors.lastname: Lee
authors.affiliation: 1] Translational Research Center and Inha Research Institute for Medical Sciences, Inha University School of Medicine, Incheon 400-712, Republic of Korea [2].
authors.forename: TacGhee
authors.initials: T
authors.lastname: Yi
authors.affiliation: College of Pharmacy and Research Institute of Pharmaceutical Sciences, Seoul National University, Seoul 151-742, Republic of Korea.
authors.forename: Kyung-Eun
authors.initials: KE
authors.lastname: Park
authors.affiliation: Drug Development Program, Department of Medicine, Inha University School of Medicine, Incheon 400-712, Republic of Korea.
authors.forename: Hyun-Joo
authors.initials: HJ
authors.lastname: Lee
authors.affiliation: Translational Research Center and Inha Research Institute for Medical Sciences, Inha University School of Medicine, Incheon 400-712, Republic of Korea.
authors.forename: Yun-Kyoung
authors.initials: YK
authors.lastname: Cho
authors.affiliation: College of Pharmacy and Research Institute of Pharmaceutical Sciences, Seoul National University, Seoul 151-742, Republic of Korea.
authors.forename: Seul Ji
authors.initials: SJ
authors.lastname: Lee
authors.affiliation: School of Pharmacy, Sungkyunkwan University, Suwon 440-746, Republic of Korea.
authors.forename: Jeongmi
authors.initials: J
authors.lastname: Lee
authors.affiliation: College of Pharmacy and Research Institute of Pharmaceutical Sciences, Seoul National University, Seoul 151-742, Republic of Korea.
authors.forename: Jeong Hill
authors.initials: JH
authors.lastname: Park
authors.affiliation: Occupational Safety and Health Research Institute, Korea Occupational Safety and Health Agency Incheon 403-711, Republic of Korea.
authors.forename: Mi-Young
authors.initials: MY
authors.lastname: Lee
authors.affiliation: Translational Research Center and Inha Research Institute for Medical Sciences, Inha University School of Medicine, Incheon 400-712, Republic of Korea.
authors.forename: Sun U
authors.initials: SU
authors.lastname: Song
authors.affiliation: College of Pharmacy and Research Institute of Pharmaceutical Sciences, Seoul National University, Seoul 151-742, Republic of Korea.
authors.forename: Sung Won
authors.initials: SW
authors.lastname: Kwon
mesh_terms: D000273:Adipose Tissue / Q000166:cytology / Q000187:drug effects*; D001854:Bone Marrow Cells / Q000166:cytology / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects*; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D007848:Laurates / Q000008:administration & dosage; D008041:Linoleic Acids / Q000008:administration & dosage; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects*; D009829:Oleic Acids / Q000008:administration & dosage; D010168:Palmitates / Q000008:administration & dosage; D052617:Solid Phase Microextraction
chemical_list: D007848:Laurates; D008041:Linoleic Acids; D009829:Oleic Acids; D010168:Palmitates; C005575:methyl linoleate; C005576:methyl oleate; C089549:lauric acid methyl ester; C019012:methyl palmitate