title: Gene cloning and induced expression pattern of IRF4 and IRF10 in the Asian swamp eel (Monopterus albus).
abstract: The Asian swamp eel (Monopterus albus) is one of the most economically important freshwater fish in East Asia, but data on the immune genes of M. albus are scarce compared to other commercially important fish. A better understanding of the eel's immune responses may help in developing strategies for disease management, potentially improving yields and mitigating losses. In mammals, interferon regulatory factors (IRFs) play a vital role in both the innate and adaptive immune system; though among teleosts IRF4 and IRF10 have seldom been studied. In this study, we characterized IRF4 and IRF10 from M. albus (maIRF4 and maIRF10) and found that maIRF4 cDNA consists of 1 716 nucleotides encoding a 451 amino acid (aa) protein, while maIRF10 consists of 1 744 nucleotides including an open reading frame (ORF) of 1 236 nt encoding 411 aa. The maIRF10 gene was constitutively expressed at high levels in a variety of tissues, while maIRF4 showed a very limited expression pattern. Expression of maIRF4 and maIRF10 in head kidney, and spleen tissues was significantly up-regulated from 12 h to 48 h post-stimulation with polyinosinic: polycytidylic acid (poly I:C), lipopolysaccharide (LPS) and a common pathogenic bacteria Aeromonas hydrophila. These results suggest that IRF4 and IRF10 play roles in immune responses to both viral and bacterial infections in M. albus.
authors.affiliation: School of Animal Science, Yangtze University, Jingzhou 434020, China; State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China; Hubei Collaborative Innovation Center for Freshwater Aquaculture, Wuhan 430070, China. xuqiaoqing@yangtzeu.edu.cn.
authors.forename: Qiao-Qing
authors.initials: QQ
authors.lastname: Xu
authors.affiliation: School of Animal Science, Yangtze University, Jingzhou 434020, China; Hubei Collaborative Innovation Center for Freshwater Aquaculture, Wuhan 430070, China.
authors.forename: Dai-Qin
authors.initials: DQ
authors.lastname: Yang
authors.affiliation: School of Animal Science, Yangtze University, Jingzhou 434020, China;
authors.forename: Rui
authors.initials: R
authors.lastname: Tuo
authors.affiliation: School of Animal Science, Yangtze University, Jingzhou 434020, China;
authors.forename: Jing
authors.initials: J
authors.lastname: Wan
authors.affiliation: State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China.
authors.forename: Ming-Xian
authors.initials: MX
authors.lastname: Chang
authors.affiliation: State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China.
authors.forename: Pin
authors.initials: P
authors.lastname: Nie
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D004524:Eels / Q000235:genetics*; D029941:Fish Proteins / Q000235:genetics / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000502:physiology; D050835:Interferon Regulatory Factors / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D010802:Phylogeny; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D059467:Transcriptome
chemical_list: D004268:DNA-Binding Proteins; D029941:Fish Proteins; D050835:Interferon Regulatory Factors; D014157:Transcription Factors
keywords: IRF10; IRF4; Monopterus albus; Poly I:C; Up-regulation