title: Measuring soluble ICAM-1 in African populations.
abstract: The level of plasma soluble ICAM-1 (sICAM-1) has been associated with the pathogenesis of several diseases. Previously, a commercial antibody was reported not to recognize an ICAM-1 allele known as ICAM-1kilifi prevalent among African populations. However, that study was based on 19 samples from African Americans of whom 13 had the wild type allele, five heterozygotes and one homozygote. Here, we compare plasma sICAM-1 measures using three different commercial antibodies in samples from Kenyan children genotyped for ICAM-1kilifi allele. We show that two of these antibodies have some degree of deficiency in detecting the ICAM-1kilifi allele. Consideration of the antibody used to measure sICAM-1 is important as up to 30% of the populations in Africa harbour this allele.
authors.affiliation: KEMRI-Wellcome Trust Research Programme, Kilifi, Kenya; Department of Biochemistry and Chemistry, Pwani University, Kilifi, Kenya.
authors.forename: Abdirahman I
authors.initials: AI
authors.lastname: Abdi
authors.affiliation: KEMRI-Wellcome Trust Research Programme, Kilifi, Kenya.
authors.forename: Michelle
authors.initials: M
authors.lastname: Muthui
authors.affiliation: KEMRI-Wellcome Trust Research Programme, Kilifi, Kenya.
authors.forename: Esther
authors.initials: E
authors.lastname: Kiragu
authors.affiliation: KEMRI-Wellcome Trust Research Programme, Kilifi, Kenya; Nuffield Department of Clinical Medicine, John Radcliffe Hospital, University of Oxford, Oxford, United Kingdom.
authors.forename: Peter C
authors.initials: PC
authors.lastname: Bull
mesh_terms: D000483:Alleles; D044383:Black People / Q000235:genetics; D005260:Female; D005838:Genotype; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000097:blood* / Q000235:genetics; D007630:Kenya; D008297:Male; D011110:Polymorphism, Genetic; D011159:Population Surveillance; D001741:Black or African American
chemical_list: D018799:Intercellular Adhesion Molecule-1