title: Dispersive solvent-free ultrasound-assisted ionic liquid dispersive liquid-liquid microextraction coupled with HPLC for determination of ulipristal acetate.
abstract: In this paper, a simple and efficient ultrasound-assisted ionic liquid dispersive liquid-liquid microextraction (UA IL-DLLME) coupled with high-performance liquid chromatography for the analysis of ulipristal acetate (UPA) was developed. UPA could be easily migrated into 1-octyl-3-methylimidazolium hexafluorophosphate [C8mimPF6] IL phase without dispersive solvent. The research of extraction mechanism showed that hydrophobic interaction force played a key role in the IL-DLLME. Several important parameters affecting the extraction recovery were optimized. Under the optimized conditions, 25-fold enrichment factor was obtained and the limit of detection (LOD) was 6.8 ng mL(-1) (tablet) or 9.3 ng mL(-1) (serum) at a signal-to-noise ratio of 3. The calibration curve was linear over the range of 0.03-6.0 µg mL(-1). The proposed method was successfully applied to the UPA tablets and the real mice serum samples.
authors.affiliation: College of Chemistry & Chemical Engineering, Yangzhou University, Yangzhou 225002, China; Yangzhou Polytechnic Institute, Yangzhou 225127, China.
authors.forename: Aiqin
authors.initials: A
authors.lastname: Gong
authors.affiliation: College of Chemistry & Chemical Engineering, Yangzhou University, Yangzhou 225002, China. Electronic address: xszhu@yzu.edu.cn.
authors.forename: Xiashi
authors.initials: X
authors.lastname: Zhu
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D007093:Imidazoles / Q000737:chemistry*; D052578:Ionic Liquids / Q000737:chemistry*; D057230:Limit of Detection; D059627:Liquid Phase Microextraction / Q000379:methods*; D051379:Mice; D009649:Norpregnadienes / Q000032:analysis* / Q000302:isolation & purification*; D044967:Serum / Q000737:chemistry; D013607:Tablets / Q000032:analysis; D014465:Ultrasonics / Q000379:methods*
chemical_list: C446740:1-octyl-3-methylimidazolium hexafluorophosphate; D007093:Imidazoles; D052578:Ionic Liquids; D009649:Norpregnadienes; D013607:Tablets; C555622:ulipristal acetate
keywords: HPLC; Ionic liquid; Liquid–liquid microextraction; Ulipristal acetate