title: Research on Toxocara canis antibodies obtained from patients with eosinophilia.
abstract: BACKGROUND
  Eosinophils may suggest the presence of a great variety of anomalies whereupon allergies, malignancies, certain tissue disorders, idiopathic hypereosynophilic syndrome and parasitic infections (with the exception of protozoons) can be cited as a few. Although the clinical manifestations may differ, the eosinophils level is quite an important data in cases related to the helminth infections. Similarly, in parasitic infections related to larva migrans (visceral, cutaneous, ocular), the eosinophils level is again a primary indicator and its evident cause is the roundworm Toxocara spp.
  
  
  OBJECTIVE
  The aim of this study was to evaluate the significance characteristics of Toxocara spp. in patients with eosonophilia.
  
  
  METHODS
  In our study, serums were collected from 93 patients of various age groups with eosonophilia (10% and above) while visiting Istanbul University Medical Faculty due to various complaints.
  
  
  RESULTS
  Serum samples were treated with Toxocara IgG ready ELISA kit and Toxocara western blot IgG ready kit. Based on the ELISA method; out of 93 patients, 30 patients (32.3%) were positive. Western blot technique; 45 (48.4%) were positive with anti-toxocara IgG antibodies.
  
  
  CONCLUSIONS
  Results point out to western blot technique being more sensitive and superior on a scale of (P < 0.0001) when compared with the ELISA method.
authors.forename: E
authors.initials: E
authors.lastname: Artinyan
authors.affiliation: Department of Parasitology, Medical Microbiology, Istanbul Faculty of Medicine, Istanbul University Istanbul, Turkey.
authors.forename: H Kirkoyun
authors.initials: HK
authors.lastname: Uysal
authors.forename: O
authors.initials: O
authors.lastname: Akgul
authors.forename: S
authors.initials: S
authors.lastname: Altiparmak
authors.forename: Y A
authors.initials: YA
authors.lastname: Oner
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood*; D015153:Blotting, Western / Q000379:methods*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D004802:Eosinophilia / Q000209:etiology* / Q000276:immunology*; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D012680:Sensitivity and Specificity; D017166:Toxocara canis / Q000276:immunology*; D014120:Toxocariasis / Q000175:diagnosis* / Q000276:immunology*
chemical_list: D000909:Antibodies, Helminth; D007074:Immunoglobulin G