title: Fabrication of highly cross-linked reversed-phase monolithic columns via living radical polymerization.
abstract: New monolithic reversed-phase liquid chromatography (RPLC) stationary phases based on single multi-acrylate/methacrylate-containing monomers [i.e., 1,12-dodecanediol dimethacrylate (1,12-DoDDMA), trimethylolpropane trimethacrylate (TRIM) and pentaerythritol tetraacrylate (PETA)] were synthesized using organotellurium-mediated living radical polymerization (TERP), which was expected to produce more efficient monolithic columns than conventional free-radical polymerization. The rationale behind selection of porogens, relative concentrations of reagents and polymerization conditions are described. The new monolithic columns were applied to the separation of small molecules (i.e., alkylbenzenes) under isocratic conditions. Chromatographic efficiencies as high as 60,200 plates/m (71,300 plates/m when corrected for extra-column variance) were obtained, showing a general improvement over previous RPLC monoliths.
authors.affiliation: Department of Chemistry and Biochemistry, Brigham Young University, Provo, UT 84602, USA.
authors.forename: Kun
authors.initials: K
authors.lastname: Liu
authors.affiliation: Department of Chemistry and Biochemistry, Brigham Young University, Provo, UT 84602, USA.
authors.forename: Pankaj
authors.initials: P
authors.lastname: Aggarwal
authors.affiliation: Department of Statistics, Brigham Young University, Provo, UT 84602, USA.
authors.forename: H Dennis
authors.initials: HD
authors.lastname: Tolley
authors.affiliation: Department of Statistics, Brigham Young University, Provo, UT 84602, USA.
authors.forename: John S
authors.initials: JS
authors.lastname: Lawson
authors.affiliation: Department of Chemistry and Biochemistry, Brigham Young University, Provo, UT 84602, USA. Electronic address: Milton_lee@byu.edu.
authors.forename: Milton L
authors.initials: ML
authors.lastname: Lee
mesh_terms: D000179:Acrylates / Q000737:chemistry; D056148:Chromatography, Reverse-Phase / Q000295:instrumentation*; D008689:Methacrylates / Q000737:chemistry; D008855:Microscopy, Electron, Scanning; D058105:Polymerization; D011109:Polymethacrylic Acids / Q000737:chemistry; D011409:Propylene Glycols / Q000737:chemistry
chemical_list: D000179:Acrylates; D008689:Methacrylates; D011109:Polymethacrylic Acids; D011409:Propylene Glycols; C098757:1,12-dodecanediol dimethacrylate; C531365:pentaerythritol tetraacrylate; C028643:trimethylolpropane trimethacrylate
keywords: Capillary columns; Liquid chromatography; Living radical polymerization; Organotellurium; Polymeric monoliths; Small molecules