title: Diallyl sulfide inhibits diethylstilbestrol induced DNA damage in human breast epithelial cells (MCF-10A).
abstract: Breast cancer is the second leading cause of cancer deaths in women in the United States. Diethylstilbestrol (DES) is a synthetic estrogen that has been shown to cause cancer in animals and humans, altering cell viability as well as inducing DNA damage. Diallyl sulfide (DAS) is a garlic organosulfide that has been shown to inhibit both the initiation and promotion phases of cancer in vivo and in vitro, as well as reduce the risk of cancer in epidemiological studies. MCF-10A cells, regarded as a normal breast epithelial cell line, were treated with varying concentrations of DES, DAS or various dose combinations of DES and DAS concomitantly, and assessed for cell viability, DNA strand breaks, and lipid peroxidation. DES (10μM) in combination with 1, 10, or 100μM DAS resulted in a 31%, 34%, or 36% respective increase in cell viability compared to the DES treatment alone, after 24h. At the same time point, 1, 10, and 100μM DAS were all effective in significantly reducing DES (100μM)-induced strand breaks to near that of the vehicle control. Additionally, 1μM DAS was effective in significantly reducing DES (100μM)-induced lipid peroxidation after 3h. The results of this research suggest that DAS is effective in recovering cell viability, attenuating DNA strand breaks, and decreasing lipid peroxidation in MCF-10A cells.
authors.affiliation: Pharmacology/Toxicology Department, Basic Pharmaceutical Sciences Division, College of Pharmacy and Pharmaceutical Science, Florida A&M University, Tallahassee, FL 32307, United States; Global Environmental Health Sciences, School of Public Health and Tropical Medicine, Tulane University, New Orleans, LA 70112, United States. Electronic address: michaellmccaskill@msn.com.
authors.forename: Michael L
authors.initials: ML
authors.lastname: McCaskill
authors.affiliation: Environmental Agricultural and Occupational Health Department, College of Public Health, University of Nebraska Medical Center, Omaha, NE 68198, United States. Electronic address: egrogan@unmc.edu.
authors.forename: Eleanor
authors.initials: E
authors.lastname: Rogan
authors.affiliation: Pharmacology/Toxicology Department, Basic Pharmaceutical Sciences Department, College of Pharmacy and Pharmaceutical Science, Florida A&M University, Tallahassee, FL 32307, United States.
authors.forename: Ronald D
authors.initials: RD
authors.lastname: Thomas
mesh_terms: D000498:Allyl Compounds / Q000737:chemistry / Q000494:pharmacology*; D016588:Anticarcinogenic Agents / Q000737:chemistry / Q000494:pharmacology; D001943:Breast Neoplasms / Q000378:metabolism; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D004249:DNA Damage / Q000187:drug effects; D004054:Diethylstilbestrol / Q000737:chemistry / Q000494:pharmacology*; D005260:Female; D006801:Humans; D015227:Lipid Peroxidation / Q000187:drug effects; D013440:Sulfides / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D000498:Allyl Compounds; D016588:Anticarcinogenic Agents; D013440:Sulfides; C038491:allyl sulfide; D004054:Diethylstilbestrol
keywords: Breast epithelial cells chemoprevention; DNA damage; Diallyl sulfide; Diethystilbestrol