title: Determining auditory-evoked activities from multiple cells in layer 1 of the dorsal cortex of the inferior colliculus of mice by in vivo calcium imaging.
abstract: Layer 1 of the dorsal cortex of the inferior colliculus (DCIC) is distinguished from other layers by its cytoarchitecture and fiber connections. However, the information of the sound types represented in layer 1 of the DCIC remains unclear because placing electrodes on such thin structures is challenging. In this study, we utilized in vivo calcium imaging to assess auditory-evoked activities in multiple cells in layer 1 of DCIC and to characterize sound stimuli producing strong activity. Most cells examined showed strong responses to broad-band noise and low-frequency tone bursts of high sound intensity. In some cases, we successfully obtained frequency response areas, which are receptive fields to tone frequencies and intensities, and ~30% of these showed V-shape tunings. This is the first systematic study to record auditory responses of cells in layer 1 of DCIC. These results indicate that cells in this area are selective to tones with low frequency, implying the importance of such auditory information in the neural circuitry of layer 1 of DCIC.
authors.affiliation: Department of Anatomy, Faculty of Medical Sciences, University of Fukui, Eiheiji, Fukui 910-1193, Japan; Research and Education Program for Life Science, University of Fukui, Fukui, Fukui 910-8507, Japan. Electronic address: itot@u-fukui.ac.jp.
authors.forename: Tetsufumi
authors.initials: T
authors.lastname: Ito
authors.affiliation: Department of Human and Artificial Intelligence Systems, Graduate School of Engineering, University of Fukui, Fukui, Fukui 910-8507, Japan.
authors.forename: Junichi
authors.initials: J
authors.lastname: Hirose
authors.affiliation: Research and Education Program for Life Science, University of Fukui, Fukui, Fukui 910-8507, Japan; Department of Human and Artificial Intelligence Systems, Graduate School of Engineering, University of Fukui, Fukui, Fukui 910-8507, Japan.
authors.forename: Kazuyuki
authors.initials: K
authors.lastname: Murase
authors.affiliation: Research and Education Program for Life Science, University of Fukui, Fukui, Fukui 910-8507, Japan; Department of Human and Artificial Intelligence Systems, Graduate School of Engineering, University of Fukui, Fukui, Fukui 910-8507, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Ikeda
mesh_terms: D000161:Acoustic Stimulation; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002452:Cell Count; D004567:Electrodes, Implanted; D005072:Evoked Potentials, Auditory / Q000502:physiology*; D005260:Female; D007245:Inferior Colliculi / Q000166:cytology / Q000502:physiology*; D051379:Mice; D008807:Mice, Inbred BALB C; D018613:Microscopy, Confocal
chemical_list: D002118:Calcium
keywords: Frequency response area; In vivo calcium imaging; Inferior colliculus; Layer 1 of the dorsal cortex