title: Skin-nerve preparation to assay the function of opioid receptors in peripheral endings of sensory neurons.
abstract: This chapter describes the methodology of the in vitro skin-saphenous nerve preparation and its application to test for the modulatory effects of opioids on the function of cutaneous sensory neurons in experimental models of pain. We detail the skin-nerve setup requirements and the technique to record action potentials from single sensory fibers. We address how to test for inhibitory effects of opioid receptor activation on mechanical and thermal sensitivity of nociceptors and mechanoreceptors in the complete Freund's adjuvant-induced inflammation and the chronic constriction injury model of neuropathic pain.
authors.affiliation: Klinik für Anästhesiologie und Operative Intensivmedizin, Freie Universität Berlin, Charité-Universitätsmedizin Berlin, Campus Mitte and Virchow-Klinikum, Augustenburger Platz 1, Berlin, 13353, Germany, rabih.moshourab@charite.de.
authors.forename: Rabih
authors.initials: R
authors.lastname: Moshourab
authors.forename: Yvonne
authors.initials: Y
authors.lastname: Schmidt
authors.forename: Halina
authors.initials: H
authors.lastname: Machelska
mesh_terms: D000701:Analgesics, Opioid / Q000008:administration & dosage*; D000818:Animals; D051379:Mice; D009417:Nerve Tissue / Q000187:drug effects; D009437:Neuralgia / Q000503:physiopathology; D009619:Nociceptors / Q000187:drug effects; D010147:Pain Measurement; D051381:Rats; D011957:Receptors, Opioid / Q000378:metabolism*; D011984:Sensory Receptor Cells / Q000187:drug effects* / Q000378:metabolism; D012867:Skin / Q000187:drug effects
chemical_list: D000701:Analgesics, Opioid; D011957:Receptors, Opioid