title: Visualization of epigenetic modifications in preimplantation embryos.
abstract: Epigenetic modifications such as DNA methylation and posttranslational histone modifications change drastically during embryonic development. To visualize histone modifications in living embryos, a Fab-based live endogenous modification labeling (FabLEM) technique has been developed. Here we describe the methods required for FabLEM experiments, including Fab preparation from IgG, its conjugation with a fluorescent dye, loading into cultured cells or mouse embryos, and imaging.
authors.affiliation: Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, 4259 Nagatsuta-cho, Midori-ku, Yokohama, 226-8501, Japan, hkimura@bio.titech.ac.jp.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kimura
authors.forename: Kazuo
authors.initials: K
authors.lastname: Yamagata
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000502:physiology*; D002478:Cells, Cultured; D003584:Cytological Techniques / Q000379:methods*; D044127:Epigenesis, Genetic; D005260:Female; D005456:Fluorescent Dyes / Q000032:analysis / Q000737:chemistry; D006367:HeLa Cells; D006801:Humans; D007091:Image Processing, Computer-Assisted; D007140:Immunoglobulin Fab Fragments / Q000378:metabolism; D051379:Mice; D008815:Mice, Inbred Strains; D008845:Microinjections
chemical_list: D005456:Fluorescent Dyes; D007140:Immunoglobulin Fab Fragments