title: AltMV TGB1 Nucleolar Localization Requires Homologous Interaction and Correlates with Cell Wall Localization Associated with Cell-to-Cell Movement.
abstract: The Potexvirus Alternanthera mosaic virus (AltMV) has multifunctional triple gene block (TGB) proteins, among which our studies have focused on the properties of the TGB1 protein. The TGB1 of AltMV has functions including RNA binding, RNA silencing suppression, and cell-to-cell movement, and is known to form homologous interactions. The helicase domains of AltMV TGB1 were separately mutated to identify which regions are involved in homologous TGB1 interactions. The yeast two hybrid system and Bimolecular Fluorescence Complementation (BiFC) in planta were utilized to examine homologous interactions of the mutants. Helicase motif I of AltMV TGB1 was found to be critical to maintain homologous interactions. Mutations in the remaining helicase motifs did not inhibit TGB1 homologous interactions. In the absence of homologous interaction of TGB1, subcellular localization of helicase domain I mutants showed distinctively different patterns from that of WT TGB1. These results provide important information to study viral movement and replication of AltMV.
authors.affiliation: Department of Applied Biology, Chungnam National University, Daejeon 305-764, Korea ; Department of Bioscience II, Bio-Medical Science, Daejeon 305-301, Korea.
authors.forename: Jiryun
authors.initials: J
authors.lastname: Nam
authors.affiliation: Department of Applied Biology, Chungnam National University, Daejeon 305-764, Korea.
authors.forename: Moon
authors.initials: M
authors.lastname: Nam
authors.affiliation: Department of Biological Science, Youngnam University, Gyeongsan 712-749, Korea.
authors.forename: Hanhong
authors.initials: H
authors.lastname: Bae
authors.affiliation: Department of Chemical and Biological Engineering, Seokyoung University, Seoul 136-704, Korea.
authors.forename: Cheolho
authors.initials: C
authors.lastname: Lee
authors.affiliation: Crop Environment Research Division, National Institute of Crop Science, RDA, Suwon 441-100, Korea.
authors.forename: Bong-Chun
authors.initials: BC
authors.lastname: Lee
authors.affiliation: USDA-ARS, US National Arboretum, Floral and Nursery Plants Research Unit, Beltsville, MD 20705, USA.
authors.forename: John
authors.initials: J
authors.lastname: Hammond
authors.affiliation: Department of Applied Biology, Chungnam National University, Daejeon 305-764, Korea.
authors.forename: Hyoun-Sub
authors.initials: HS
authors.lastname: Lim
keywords: AltMV; TGB1; homologous interaction; subcellular localization