title: Development of a monoclonal antibody-based indirect competitive immunosorbent assay for 4(5)-Methylimidazole detection in caramels.
abstract: In this study, an indirect competitive enzyme-linked immunoassay (ic-ELISA) based on monoclonal antibody for 4(5)-Methylimidazole (4-MI) detection was described. The artificial antigens were prepared by conjugating bovine serum albumin (BSA) or ovalbumin (OVA) with the hapten of 4-MI. And monoclonal antibody, evaluated by ic-ELISA, was obtained by immunizing BABL/c mice. After optimizing, a standard curve for ic-ELISA detection on 4-MI was obtained with the linear detection range of 0.64-20.48 mg/L. The cross-reactivity (CR) of all the structural analogues of 4-MI was less than 5.62%. The recoveries of 4-MI in caramels detection were ranged from 88.69% to 114.09%, with relative standard deviation (n=3) below 8.07%. The results suggested that the established ic-ELISA is promising for 4-MI commercial detection in caramels.
authors.affiliation: College of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510640, China.
authors.forename: Xin-Lan
authors.initials: XL
authors.lastname: Wu
authors.affiliation: College of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510640, China; Guangdong Province Key Laboratory for Green Processing of Natural Products and Product Safety, Guangzhou 510640, China. Electronic address: shujuanyu8@gmail.com.
authors.forename: Shu-Juan
authors.initials: SJ
authors.lastname: Yu
authors.affiliation: Guangzhou Wondfo Biotech Co., Ltd., Guangzhou 510663, China.
authors.forename: Ke-Ren
authors.initials: KR
authors.lastname: Kang
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D001681:Biological Assay; D002182:Candy / Q000032:analysis*; D002241:Carbohydrates; D007164:Immunosorbents; D051379:Mice; D008807:Mice, Inbred BALB C
chemical_list: D000911:Antibodies, Monoclonal; D002241:Carbohydrates; D007164:Immunosorbents; C569050:caramel coloring
keywords: 1,2-Dimethylimidazole (PubChem CID: 5617); 1-Methylimidazole (PubChem CID: 1390); 2-Methylimidazole (PubChem CID: 12749); 4(5)-Methylimidazole; 4(5)-Methylimidazole (PubChem CID: 13195); 4-Nitrobenzyl bromide (PubChem CID: 66011); 5-Benzimidazolecarboxylic acid (PubChem CID: 459456); Histamine (PubChem CID: 774); Monoclonal antibodies; ic-ELISA