title: A novel thiolated human-like collage zinc complex as a promising zinc supplement: physicochemical characteristics and biocompatibility.
abstract: To improve zinc binding ability to human-like collagen (HLC) and stability of metal complex, HLC was thiolated by mercaptosuccinylation reaction with S-acetylmercaptosuccinic anhydride (S-AMSA) at pH8.0. One mole of thiolated HLC-Zn (SHLC-Zn) complex possessed 24.3mol zinc ions when pH was 8.0 and zinc concentration was 15 mM. The physicochemical properties and biocompatibility of thiolated HLC-Zn (SHLC-Zn) complex were investigated by UV-vis, CD, electrophoresis analysis, differential scanning calorimetry (DSC) and cell viability assay, respectively. The results showed that SHLC-Zn complex(1) exhibited higher zinc ions than that of native HLC and still maintained the secondary structure of HLC though interaction occurred between SHLC and zinc ions, (2) increased the apparent molecular weight when compared with native HLC, (3) exhibited greater thermal stability than native HLC, and (4) presented toxicity free for BHK cells. This study suggests that the SHLC-Zn complex is a potential nutrition as well as zinc supplement in the medical application.
authors.affiliation: Shaanxi Key laboratory of Degradable Biomedical Materials, School of Chemical Engineering, Northwest University, Xi'an 710069, China; Shaanxi R&D Center of Biomaterials and Fermentation Engineering, School of Chemical Engineering, Northwest University, Xi'an 710069, China.
authors.forename: Chenhui
authors.initials: C
authors.lastname: Zhu
authors.affiliation: Shaanxi Key laboratory of Degradable Biomedical Materials, School of Chemical Engineering, Northwest University, Xi'an 710069, China; Shaanxi R&D Center of Biomaterials and Fermentation Engineering, School of Chemical Engineering, Northwest University, Xi'an 710069, China.
authors.forename: Xiaoxuan
authors.initials: X
authors.lastname: Ma
authors.affiliation: Shaanxi Key laboratory of Degradable Biomedical Materials, School of Chemical Engineering, Northwest University, Xi'an 710069, China; Shaanxi R&D Center of Biomaterials and Fermentation Engineering, School of Chemical Engineering, Northwest University, Xi'an 710069, China.
authors.forename: Yonghui
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Shaanxi Key laboratory of Degradable Biomedical Materials, School of Chemical Engineering, Northwest University, Xi'an 710069, China; Shaanxi R&D Center of Biomaterials and Fermentation Engineering, School of Chemical Engineering, Northwest University, Xi'an 710069, China. Electronic address: mi_yu@nwu.edu.cn.
authors.forename: Yu
authors.initials: Y
authors.lastname: Mi
authors.affiliation: Shaanxi Key laboratory of Degradable Biomedical Materials, School of Chemical Engineering, Northwest University, Xi'an 710069, China; Shaanxi R&D Center of Biomaterials and Fermentation Engineering, School of Chemical Engineering, Northwest University, Xi'an 710069, China. Electronic address: fandaidi@nwu.edu.cn.
authors.forename: Daidi
authors.initials: D
authors.lastname: Fan
authors.affiliation: Shaanxi Key laboratory of Degradable Biomedical Materials, School of Chemical Engineering, Northwest University, Xi'an 710069, China; Shaanxi R&D Center of Biomaterials and Fermentation Engineering, School of Chemical Engineering, Northwest University, Xi'an 710069, China.
authors.forename: Jianjun
authors.initials: J
authors.lastname: Deng
authors.affiliation: Shaanxi Microbial Institute, Xi'an 710043, China.
authors.forename: Wenjiao
authors.initials: W
authors.lastname: Xue
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D002152:Calorimetry, Differential Scanning; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D055598:Chemical Phenomena; D002942:Circular Dichroism; D003094:Collagen / Q000737:chemistry; D056831:Coordination Complexes / Q000737:chemistry; D006224:Cricetinae; D006863:Hydrogen-Ion Concentration; D017433:Protein Structure, Secondary; D013387:Succinic Anhydrides / Q000737:chemistry; D015032:Zinc / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials; D056831:Coordination Complexes; D013387:Succinic Anhydrides; C024249:S-acetylmercaptosuccinic anhydride; D003094:Collagen; D015032:Zinc
keywords: Human-like collagen; S-acetylmercaptosuccinic anhydride; Zinc