title: Expression, crystallization and preliminary X-ray crystallographic analysis of cystathionine β-lyase from Acinetobacter baumannii OXA-23.
abstract: Multidrug-resistant Acinetobacter baumannii (Ab) has emerged as a leading nosocomial pathogen because of its resistance to most currently available antibiotics. Cystathionine β-lyase (CBL), a pyridoxal 5'-phosphate (PLP)-dependent enzyme, catalyzes the second step in the transsulfuration pathway, which is essential for the metabolic interconversion of the sulfur-containing amino acids homocysteine and methionine. The enzymes of the transsulfuration pathway are considered to be attractive drug targets owing to their specificity to microbes and plants. As a potential target for the development of novel antibacterial drugs, the AbCBL protein was expressed, purified and crystallized. An AbCBL crystal diffracted to 1.57 Å resolution and belonged to the trigonal space group P3112, with unit-cell parameters a = b = 102.9, c = 136.5 Å. The asymmetric unit contained two monomers, with a corresponding VM of 2.3 Å(3) Da(-1) and a solvent content of 46.9%.
authors.affiliation: Department of Biological Sciences, Konkuk University, 1 Hwayang dong, Gwangjin-gu, Seoul 143-701, Republic of Korea.
authors.forename: Diem Quynh
authors.initials: DQ
authors.lastname: Nguyen
authors.affiliation: Department of Biological Sciences, Konkuk University, 1 Hwayang dong, Gwangjin-gu, Seoul 143-701, Republic of Korea.
authors.forename: Ho Phuong Thuy
authors.initials: HP
authors.lastname: Ngo
authors.affiliation: Department of Life Science, College of Natural Sciences, Sangmyung University, 7 Hongji-dong, Jongno-gu, Seoul 110-743, Republic of Korea.
authors.forename: Yeh Jin
authors.initials: YJ
authors.lastname: Ahn
authors.affiliation: National Leading Research Laboratory of Drug Resistance Proteomics, Department of Biological Sciences, Myongji University, 116 Myongji-ro, Yongin, Gyeonggido 449-728, Republic of Korea.
authors.forename: Sang Hee
authors.initials: SH
authors.lastname: Lee
authors.affiliation: Department of Biological Sciences, Konkuk University, 1 Hwayang dong, Gwangjin-gu, Seoul 143-701, Republic of Korea.
authors.forename: Lin Woo
authors.initials: LW
authors.lastname: Kang
mesh_terms: D040981:Acinetobacter baumannii / Q000201:enzymology*; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000737:chemistry*; D003460:Crystallization; D018360:Crystallography, X-Ray; D004926:Escherichia coli; D015870:Gene Expression; D008190:Lyases / Q000096:biosynthesis / Q000737:chemistry*; D008969:Molecular Sequence Data; D011732:Pyridoxal Phosphate / Q000737:chemistry
chemical_list: D001426:Bacterial Proteins; D011732:Pyridoxal Phosphate; D008190:Lyases; C019965:cystathionine beta-lyase
keywords: PLP-dependent enzyme; cystathionine β-lyase; multidrug-resistant Acinetobacter baumannii