title: Alfalfa (Medicago sativa L.).
abstract: Alfalfa (Medicago sativa L.) is a high-quality forage crop widely grown throughout the world. This chapter describes an efficient protocol that allows for the generation of large number of transgenic alfalfa plants by sonication-assisted Agrobacterium-mediated transformation. Binary vectors carrying different selectable marker genes that confer resistance to phosphinothricin (bar), kanamycin (npt II), or hygromycin (hph) were used to generate transgenic alfalfa plants. Intact trifoliates collected from clonally propagated plants in the greenhouse were sterilized with bleach and then inoculated with Agrobacterium strain EHA105. More than 80 % of infected leaf pieces could produce rooted transgenic plants in 4-5 months after Agrobacterium-mediated transformation.
authors.affiliation: Forage Improvement Division, The Samuel Roberts Noble Foundation, 2510 Sam Noble Parkway, Ardmore, OK, 73401, USA.
authors.forename: Chunxiang
authors.initials: C
authors.lastname: Fu
authors.forename: Timothy
authors.initials: T
authors.lastname: Hernandez
authors.forename: Chuanen
authors.initials: C
authors.lastname: Zhou
authors.forename: Zeng-Yu
authors.initials: ZY
authors.lastname: Wang
mesh_terms: D000383:Agriculture / Q000379:methods; D000613:Aminobutyrates / Q000494:pharmacology; D002934:Cinnamates / Q000494:pharmacology; D018920:Coculture Techniques; D004351:Drug Resistance / Q000235:genetics; D005821:Genetic Techniques; D005822:Genetic Vectors; D006921:Hygromycin B / Q000031:analogs & derivatives / Q000494:pharmacology; D007612:Kanamycin / Q000494:pharmacology; D000455:Medicago sativa / Q000187:drug effects / Q000235:genetics*; D018515:Plant Leaves / Q000235:genetics; D030821:Plants, Genetically Modified; D012639:Seeds / Q000235:genetics / Q000254:growth & development; D013242:Sterilization; D014169:Transformation, Bacterial
chemical_list: D000613:Aminobutyrates; D002934:Cinnamates; D006921:Hygromycin B; C026273:hygromycin A; C003121:phosphinothricin; D007612:Kanamycin