title: [Construction of prokaryotic expression vector for Ag85A-HA2 fusion gene and studies on the immunity efficacy of fusion protein against influenza A virus].
abstract: OBJECTIVE
  To construct Ag85A-HA2 prokaryotic expression vector, express the fusion protein and study the immunity efficacy of fusion protein against influenza A virus.
  
  
  METHODS
  Ag85A-HA2 prokaryotic expression vector was constructed and induced with IPTG. The fusion protein was identified by SDS-PAGE and purified with His-Tag affinity chromatography. The BALB/c mice were immunized with fusion protein. Then the pathological section, lung index, lung inhibitory rate and death-protection rate were tested to evaluate the immunity efficacy of fusion protein.
  
  
  RESULTS
  pET-32a(+)/Ag85A-HA2 prokaryotic expression vector was constructed successfully. And SDS-PAGE indicated that fusion protein was expressed correctly with a molecular mass of 70 x 10(3). The lung index and death-protection rate in experimental group were 39.30% and 80%, higher than that of control group. The pathological section also demonstrated that Ag85A-HA2 fusion protein had a protective effect on murine lungs.
  
  
  CONCLUSIONS
  Ag85A-HA2 prokaryotic expression vector was successfully constructed, inducible expression and the fusion protein had an immunity efficacy against influenza A virus in animal experiment.
authors.forename: Jing-Jing
authors.initials: JJ
authors.lastname: Shao
authors.forename: Jing
authors.initials: J
authors.lastname: Yang
authors.forename: Jun
authors.initials: J
authors.lastname: Dai
authors.forename: Jun-Jie
authors.initials: JJ
authors.lastname: Meng
authors.forename: De-Cui
authors.initials: DC
authors.lastname: Pei
authors.forename: Hong
authors.initials: H
authors.lastname: Li
authors.forename: Xing
authors.initials: X
authors.lastname: Pan
authors.forename: Wan-Yi
authors.initials: WY
authors.lastname: Li
mesh_terms: D000217:Acyltransferases / Q000276:immunology; D000818:Animals; D000942:Antigens, Bacterial / Q000276:immunology; D004591:Electrophoresis, Polyacrylamide Gel; D005822:Genetic Vectors; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000276:immunology; D009980:Influenza A virus; D007252:Influenza Vaccines / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D009976:Orthomyxoviridae Infections / Q000517:prevention & control*; D011993:Recombinant Fusion Proteins / Q000276:immunology*
chemical_list: D000942:Antigens, Bacterial; D019267:Hemagglutinin Glycoproteins, Influenza Virus; D007252:Influenza Vaccines; D011993:Recombinant Fusion Proteins; D000217:Acyltransferases; C112580:antigen 85A, Mycobacterium tuberculosis