title: Maternal methyl-enriched diet in rat reduced the audiogenic seizure proneness in progeny.
abstract: Audiogenic epilepsy proneness was analyzed in the progeny of rats from two strains (audiogenic seizure prone-strain "4"-and audiogenic seizure non-prone, strain "0"). Females were fed by a diet which contained substances enriched with methyl-groups during 1week before mating (MED), during pregnancy period and 1week after the delivery. This MED treatment resulted in a decrease of audiogenic seizure fit intensity, which was more evident in rats of strain "0". Control rats of strain "4" displayed intense seizures (tonic seizure, 3.85 arbitrary units). Med "4" rats seizures were less intense (3.23, tonic seizure of lower intensity), control "0" strain rats demonstrated the seizure with mean 3.09 arbitrary units, "0" MED rats only 2.03 arbitrary unit intensity (only clonic seizures, significantly, p<0.05, different from controls). Methyl-enriched diet resulted in the significant changes in methylation status of several genes (Cpne6, Gtf2i, Sctr,1 Sfmbt, Phe2). These genes among others were chosen for analysis as their expression was analyzed in other methylation study. These genes were hypermethylated after "epileptic tolerance". Due to this procedure, the intensity of status epilepticus, produced by kainate in mice, decreased (Miller-Delaney et al., 2012). The modulation of audiogenic seizure intensity as the result of methyl-enriched diet during prenatal and early postnatal ontogeny was demonstrated for the first time.
authors.affiliation: Biology Faculty, Moscow State University, Leninskie Gori, 1, build. 12, Moscow 119992, Russia. Electronic address: ingapoletaeva@mail.ru.
authors.forename: I I
authors.initials: II
authors.lastname: Poletaeva
authors.affiliation: Biology Faculty, Moscow State University, Leninskie Gori, 1, build. 12, Moscow 119992, Russia.
authors.forename: N M
authors.initials: NM
authors.lastname: Surina
authors.affiliation: Belozersky Institute of Physical-Chemical Biology, Moscow State University, Leninskie Gori, 1, build. 40, Moscow 119992, Russia. Electronic address: ashapkin@genebee.msu.su.
authors.forename: V V
authors.initials: VV
authors.lastname: Ashapkin
authors.affiliation: Biology Faculty, Moscow State University, Leninskie Gori, 1, build. 12, Moscow 119992, Russia.
authors.forename: I B
authors.initials: IB
authors.lastname: Fedotova
authors.affiliation: Institute of Gene Biology, Russian Academy of Sciences, Vavilova str., 34/5, Moscow 119334, Russia.
authors.forename: I B
authors.initials: IB
authors.lastname: Merzalov
authors.affiliation: Biology Faculty, Moscow State University, Leninskie Gori, 1, build. 12, Moscow 119992, Russia.
authors.forename: O V
authors.initials: OV
authors.lastname: Perepelkina
authors.affiliation: Institute of Gene Biology, Russian Academy of Sciences, Vavilova str., 34/5, Moscow 119334, Russia.
authors.forename: G V
authors.initials: GV
authors.lastname: Pavlova
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001622:Betaine / Q000008:administration & dosage*; D002794:Choline / Q000008:administration & dosage; D004032:Diet / Q000379:methods; D020195:Epilepsy, Reflex / Q000178:diet therapy* / Q000235:genetics / Q000378:metabolism; D005260:Female; D005492:Folic Acid / Q000008:administration & dosage; D008297:Male; D008429:Maternal Welfare; D008715:Methionine / Q000008:administration & dosage*; D008745:Methylation; D011247:Pregnancy; D051381:Rats; D017208:Rats, Wistar; D014805:Vitamin B 12 / Q000008:administration & dosage
chemical_list: D001622:Betaine; D005492:Folic Acid; D008715:Methionine; D002794:Choline; D014805:Vitamin B 12
keywords: Audiogenic epilepsy; DNA methylation; Methyl enriched diet