title: Layer-by-layer-based silica encapsulation of individual yeast with thickness control.
abstract: In the area of cell-surface engineering with nanomaterials, the metabolic and functional activities of the encapsulated cells are manipulated and controlled by various parameters of the artificial shells that encase the cells, such as stiffness and elasticity, thickness, and porosity. The mechanical durability and physicochemical stability of inorganic shells prove superior to layer-by-layer-based organic shells with regard to cytoprotection, but it has been difficult to vary the parameters of inorganic shells including their thickness. In this work, we combine the layer-by-layer technique with a process of bioinspired silicification to control the thickness of the silica shells that encapsulate yeast Saccharomyces cerevisiae cells individually, and investigate the thickness-dependent microbial growth.
authors.affiliation: Center for Cell-Encapsulation Research and Molecular-Level Interface Research Center, Department of Chemistry, KAIST, Daejeon 305-701 (Korea).
authors.forename: Hojae
authors.initials: H
authors.lastname: Lee
authors.forename: Daewha
authors.initials: D
authors.lastname: Hong
authors.forename: Ji Yu
authors.initials: JY
authors.lastname: Choi
authors.forename: Ji Yup
authors.initials: JY
authors.lastname: Kim
authors.forename: Sang Hee
authors.initials: SH
authors.lastname: Lee
authors.forename: Ho Min
authors.initials: HM
authors.lastname: Kim
authors.forename: Sung Ho
authors.initials: SH
authors.lastname: Yang
authors.forename: Insung S
authors.initials: IS
authors.lastname: Choi
mesh_terms: D004548:Elasticity; D008855:Microscopy, Electron, Scanning; D049329:Nanostructures / Q000737:chemistry*; D011095:Polyethylenes / Q000737:chemistry; D016062:Porosity; D000644:Quaternary Ammonium Compounds / Q000737:chemistry; D012441:Saccharomyces cerevisiae / Q000737:chemistry* / Q000254:growth & development; D012822:Silicon Dioxide / Q000737:chemistry*
chemical_list: D011095:Polyethylenes; D000644:Quaternary Ammonium Compounds; C041004:poly-N,N-dimethyl-N,N-diallylammonium chloride; D012822:Silicon Dioxide
keywords: cell encapsulation; layer-by-layer; nanostructures; self-assembly; silicification