title: Klebsiella pneumoniae carrying bla NDM-1 gene in orthopedic practice.
abstract: Emergence and spread of carbapenemases in Enterobacteriaceae is a cause of concern worldwide, the latest threat being New Delhi metallo-β-lactamase (NDM-1). This report is of an orthopedic case with fracture femur managed with internal fixation and bone grafting, who subsequently developed secondary infection with Klebsiella pneumoniae harboring bla NDM-1 gene. Minimum inhibitory concentration (MIC) of imipenem was ≥8 μg/ml by E-test, suggestive of carbapenemase production. Phenotypic and further genotypic detection confirmed the presence of bla NDM-1 gene. The isolate remained susceptible only to tigecycline, colistin, and polymyxin B.
authors.affiliation: Department of Microbiology, Government Medical College Hospital, Sector 32, Chandigarh, India.
authors.forename: Varsha
authors.initials: V
authors.lastname: Gupta
authors.affiliation: Department of Microbiology, Government Medical College Hospital, Sector 32, Chandigarh, India.
authors.forename: Neha
authors.initials: N
authors.lastname: Bansal
authors.affiliation: Department of Orthopedics, Government Medical College Hospital, Sector 32, Chandigarh, India.
authors.forename: Ravi
authors.initials: R
authors.lastname: Gupta
authors.affiliation: Department of Microbiology, Government Medical College Hospital, Sector 32, Chandigarh, India.
authors.forename: Jagdish
authors.initials: J
authors.lastname: Chander
keywords: Carbapenemase; Klebsiella; Klebsiella pneumoniae; New Delhi metallo-β-lactamase; betalactamase; orthopedic surgery