title: Rescuing DNA repair activity by rewiring the H-atom transfer pathway in the radical SAM enzyme, spore photoproduct lyase.
abstract: The radical SAM enzyme, spore photoproduct lyase, requires an H-atom transfer (HAT) pathway to catalyze DNA repair. By rational engineering, we demonstrate that it is possible to rewire its HAT pathway, a first step toward the development of novel catalysts based on the radical SAM enzyme scaffold.
authors.affiliation: Department of Biomolecular Mechanisms, Max-Planck Institute for Medical Research, Jahnstrasse 29, 69120 Heidelberg, Germany.
authors.forename: Alhosna
authors.initials: A
authors.lastname: Benjdia
authors.forename: Korbinian
authors.initials: K
authors.lastname: Heil
authors.forename: Andreas
authors.initials: A
authors.lastname: Winkler
authors.forename: Thomas
authors.initials: T
authors.lastname: Carell
authors.forename: Ilme
authors.initials: I
authors.lastname: Schlichting
mesh_terms: D055162:Biocatalysis; D004260:DNA Repair; D056506:Geobacillus / Q000201:enzymology; D006859:Hydrogen / Q000737:chemistry / Q000378:metabolism*; D015394:Molecular Structure; D011506:Proteins / Q000737:chemistry / Q000378:metabolism*; D012436:S-Adenosylmethionine / Q000737:chemistry / Q000378:metabolism*
chemical_list: D011506:Proteins; D012436:S-Adenosylmethionine; D006859:Hydrogen; C088014:spore photoproduct lyase