title: Photodynamic therapy using nanoparticle loaded with indocyanine green for experimental peritoneal dissemination of gastric cancer.
abstract: Although there have been multiple advances in the development of novel anticancer agents and operative procedures, prognosis of patients with advanced gastric cancer remains poor, especially in patients with peritoneal metastasis. In this study, we established nanoparticles loaded with indocyanine green (ICG) derivatives: ICG loaded lactosomes (ICGm) and investigated the diagnostic and therapeutic value of photodynamic therapy (PDT) using ICGm for experimental peritoneal dissemination of gastric cancer. Experimental peritoneal disseminated xenografts of human gastric cancer were established in nude mice. Three weeks after intraperitoneal injection of the cancer cells, either ICGm (ICGm-treated mice) or ICG solution (ICG-treated mice) was injected through the tail vein. Forty-eight hours after injection of the photosensitizer, in vivo and ex vivo imaging was carried out. For PDT, 48 h after injection of the photosensitizer, other mice were irradiated through the abdominal wall, and the body weight and survival rate were monitored. In vivo imaging revealed that peritoneal tumors were visualized through the abdominal wall in ICGm-treated mice, whereas only non-specific fluorescence was observed in ICG-treated mice. The PDT reduced the total weight of the disseminated nodules and significantly improved weight loss and survival rate in ICGm-treated mice. In conclusion, ICGm can be used as a novel diagnostic and therapeutic nanodevice in peritoneal dissemination of gastric cancer.
authors.affiliation: Department of Surgery, National Defense Medical College, Tokorozawa, Japan.
authors.forename: Hironori
authors.initials: H
authors.lastname: Tsujimoto
authors.forename: Yuji
authors.initials: Y
authors.lastname: Morimoto
authors.forename: Risa
authors.initials: R
authors.lastname: Takahata
authors.forename: Shinsuke
authors.initials: S
authors.lastname: Nomura
authors.forename: Kazumichi
authors.initials: K
authors.lastname: Yoshida
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Horiguchi
authors.forename: Shuichi
authors.initials: S
authors.lastname: Hiraki
authors.forename: Satoshi
authors.initials: S
authors.lastname: Ono
authors.forename: Hiromi
authors.initials: H
authors.lastname: Miyazaki
authors.forename: Daizo
authors.initials: D
authors.lastname: Saito
authors.forename: Isao
authors.initials: I
authors.lastname: Hara
authors.forename: Eiichi
authors.initials: E
authors.lastname: Ozeki
authors.forename: Junji
authors.initials: J
authors.lastname: Yamamoto
authors.forename: Kazuo
authors.initials: K
authors.lastname: Hase
mesh_terms: D000818:Animals; D001835:Body Weight / Q000187:drug effects; D045744:Cell Line, Tumor; D006801:Humans; D007208:Indocyanine Green / Q000008:administration & dosage* / Q000737:chemistry; D051379:Mice; D008819:Mice, Nude; D053758:Nanoparticles / Q000008:administration & dosage / Q000737:chemistry* / Q000648:ultrastructure; D009374:Neoplasms, Experimental; D009928:Organ Specificity; D010529:Peritoneal Cavity / Q000293:injuries* / Q000473:pathology; D010778:Photochemotherapy; D017319:Photosensitizing Agents / Q000008:administration & dosage* / Q000737:chemistry; D013274:Stomach Neoplasms / Q000188:drug therapy* / Q000473:pathology / Q000648:ultrastructure; D016019:Survival Analysis; D023041:Xenograft Model Antitumor Assays
chemical_list: D017319:Photosensitizing Agents; D007208:Indocyanine Green
keywords: Drug delivery system; gastric cancer; nanoparticle; peritoneal dissemination; photodynamic therapy