title: Crystallization and preliminary X-ray diffraction analysis of full-length spr1814, a response regulator of Streptococcus pneumoniae, in complex with a phosphoryl analogue.
abstract: Spr1814 of Streptococcus pneumoniae is a signal transduction response regulator belonging to the NarL/FixJ subfamily, which has a helix-turn-helix DNA-binding effector domain. To understand how the phosphorylation of the conserved aspartic acid residue induces conformational changes in spr1814 allowing binding to the target promoter, recombinant spr1814 expressed in Escherichia coli was crystallized with the phosphoryl analogue beryllium fluoride BeF3(-) by the hanging-drop vapour-diffusion method. The crystals diffracted to 1.9 Å resolution and belonged to space group P21, with unit-cell parameters a = 40.2, b = 114.5, c = 50.1 Å, β = 92.1°. Structure determination by the SAD method using the bromine derivative 5-amino-2,4,6-tribromoisophthalic acid (B3C) is under way.
authors.affiliation: Division of Biotechnology, College of Life Sciences, Korea University, Seoul 136-713, Republic of Korea.
authors.forename: Aekyung
authors.initials: A
authors.lastname: Park
authors.affiliation: Division of Biotechnology, College of Life Sciences, Korea University, Seoul 136-713, Republic of Korea.
authors.forename: Jae Soon
authors.initials: JS
authors.lastname: Oh
authors.affiliation: Division of Biotechnology, College of Life Sciences, Korea University, Seoul 136-713, Republic of Korea.
authors.forename: Young Min
authors.initials: YM
authors.lastname: Chi
authors.affiliation: Korea Food Research Institute, 1201-62 Anyangpangyo-ro, Bundang-gu, Seongnam-si, Gyeonggi-do 463-746, Republic of Korea.
authors.forename: Seong Weon
authors.initials: SW
authors.lastname: Jeong
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry*; D001608:Beryllium / Q000737:chemistry*; D003460:Crystallization; D018360:Crystallography, X-Ray; D004268:DNA-Binding Proteins / Q000737:chemistry*; D005459:Fluorides / Q000737:chemistry*; D011485:Protein Binding; D013296:Streptococcus pneumoniae
chemical_list: D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; C035982:beryllium fluoride; D001608:Beryllium; D005459:Fluorides
keywords: Spr1814; Streptococcus pneumoniae