title: Crystallization and preliminary X-ray diffraction analysis of a novel sphingomyelinase D from Loxosceles gaucho venom.
abstract: Brown spider envenomation results in dermonecrosis, intravascular coagulation, haemolysis and renal failure, mainly owing to the action of sphingomyelinases D (SMases D), which catalyze the hydrolysis of sphingomyelin to produce ceramide 1-phosphate and choline or the hydrolysis of lysophosphatidylcholine to produce lysophosphatidic acid. Here, the heterologous expression, purification, crystallization and preliminary X-ray diffraction analysis of LgRec1, a novel SMase D from Loxosceles gaucho venom, are reported. The crystals belonged to space group P21212, with unit-cell parameters a = 52.98, b = 62.27, c = 84.84 Å and diffracted to a maximum resolution of 2.6 Å.
authors.affiliation: Multiuser Center for Biomolecular Innovation, Department of Physics, UNESP/IBILCE, Rua Cristovão Colombo 2265, São José Do Rio Preto, São Paulo 15054-000, Brazil.
authors.forename: Anwar
authors.initials: A
authors.lastname: Ullah
authors.affiliation: Laboratorio de Imunopatologia Divisão de Desenvolvimento Científico, Instituto Butantan, Avenida Dr Vital Brasil 1500, São Paulo 05503-900, Brazil.
authors.forename: Geraldo Santana
authors.initials: GS
authors.lastname: Magalhães
authors.affiliation: Multiuser Center for Biomolecular Innovation, Department of Physics, UNESP/IBILCE, Rua Cristovão Colombo 2265, São José Do Rio Preto, São Paulo 15054-000, Brazil.
authors.forename: Rehana
authors.initials: R
authors.lastname: Masood
authors.affiliation: Multiuser Center for Biomolecular Innovation, Department of Physics, UNESP/IBILCE, Rua Cristovão Colombo 2265, São José Do Rio Preto, São Paulo 15054-000, Brazil.
authors.forename: Ricardo Barros
authors.initials: RB
authors.lastname: Mariutti
authors.affiliation: Multiuser Center for Biomolecular Innovation, Department of Physics, UNESP/IBILCE, Rua Cristovão Colombo 2265, São José Do Rio Preto, São Paulo 15054-000, Brazil.
authors.forename: Monika Aparecida
authors.initials: MA
authors.lastname: Coronado
authors.affiliation: Laboratório Nacional de Biociências (LNBio) Centro Nacional de Pesquisa em Energia e Materiais, Campinas 13083-970, Brazil.
authors.forename: Mário Tyago
authors.initials: MT
authors.lastname: Murakami
authors.affiliation: Laboratorio de Imunopatologia Divisão de Desenvolvimento Científico, Instituto Butantan, Avenida Dr Vital Brasil 1500, São Paulo 05503-900, Brazil.
authors.forename: Katia Cristina
authors.initials: KC
authors.lastname: Barbaro
authors.affiliation: Multiuser Center for Biomolecular Innovation, Department of Physics, UNESP/IBILCE, Rua Cristovão Colombo 2265, São José Do Rio Preto, São Paulo 15054-000, Brazil.
authors.forename: Raghuvir Krishnaswamy
authors.initials: RK
authors.lastname: Arni
mesh_terms: D000595:Amino Acid Sequence; D060829:Arthropod Proteins / Q000737:chemistry*; D003460:Crystallization; D018360:Crystallography, X-Ray; D008969:Molecular Sequence Data; D010727:Phosphoric Diester Hydrolases / Q000737:chemistry*; D013111:Spider Venoms / Q000201:enzymology*
chemical_list: D060829:Arthropod Proteins; D013111:Spider Venoms; D010727:Phosphoric Diester Hydrolases; C055160:sphingomyelin phosphodiesterase D
keywords: Loxosceles gaucho; sphingomyelinase D