title: Genomic characterisation of two virulent Newcastle disease viruses isolated from crested ibis (Nipponia nippon) in China.
abstract: This paper describes the complete genomic sequences of two virulent Newcastle disease virus (NDV) isolates, Shaanxi06 (prevalent genotype VIId) and Shaanxi10 (novel sub-genotype VIi), from sick crested ibises. The genomes of both isolates were 15,192 nt long and consisted of six genes in the order of 3'-NP-P-M-F-HN-L-5'. The genomes of the two isolates were highly similar to other reference NDV strains. However, some unique features were found in the HN protein of Shaanxi06 and the F gene end of Shaanxi10. Shaanxi06 and Shaanxi10 shared the same virulent motif (112-)R-R-Q-K-R-F(-117) at the F protein cleavage site, which coincided with previous pathogenicity test results. Phylogenetic analysis revealed that both isolates were clustered within class II NDV, with Shaanxi06 in genotype VII and Shaanxi10 in genotype VI. Both isolates shared high homology with the prevalent genotype NDV strains that circulate in fowls and waterfowls. This study is the first to provide genomic information about a novel sub-genotype VIi NDV strain and another genotype VIId virus, which will be useful for subsequent investigations.
authors.affiliation: College of Veterinary Medicine, Northwest A & F University, Yangling, 712100, Shaanxi, People's Republic of China.
authors.forename: Huafang
authors.initials: H
authors.lastname: Hao
authors.affiliation: The State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 1 Xujiaping, Chengguan District, Lanzhou, 730046, Gansu, People's Republic of China.
authors.forename: Shengli
authors.initials: S
authors.lastname: Chen
authors.affiliation: College of Veterinary Medicine, Northwest A & F University, Yangling, 712100, Shaanxi, People's Republic of China.
authors.forename: Pengpeng
authors.initials: P
authors.lastname: Wu
authors.affiliation: College of Veterinary Medicine, Northwest A & F University, Yangling, 712100, Shaanxi, People's Republic of China.
authors.forename: Jie
authors.initials: J
authors.lastname: Wang
authors.affiliation: College of Veterinary Medicine, Northwest A & F University, Yangling, 712100, Shaanxi, People's Republic of China.
authors.forename: Xuji
authors.initials: X
authors.lastname: Duan
authors.affiliation: College of Veterinary Medicine, Northwest A & F University, Yangling, 712100, Shaanxi, People's Republic of China.
authors.forename: Enqi
authors.initials: E
authors.lastname: Du
authors.affiliation: College of Veterinary Medicine, Northwest A & F University, Yangling, 712100, Shaanxi, People's Republic of China.
authors.forename: Xinglong
authors.initials: X
authors.lastname: Wang
authors.affiliation: College of Veterinary Medicine, Northwest A & F University, Yangling, 712100, Shaanxi, People's Republic of China. Electronic address: yzq8162@163.com.
authors.forename: Zengqi
authors.initials: Z
authors.lastname: Yang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001717:Birds / Q000821:virology*; D002681:China; D016679:Genome, Viral; D008969:Molecular Sequence Data; D009522:Newcastle disease virus / Q000235:genetics* / Q000472:pathogenicity; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid; D014774:Virulence / Q000235:genetics
keywords: Crested ibis; Genomic sequence; Newcastle disease virus; Nipponia nippon; Phylogenetic analysis