title: Applications of flow cytometry to characterize bacterial physiological responses.
abstract: Although reports of flow cytometry (FCM) applied to bacterial analysis are increasing, studies of FCM related to human cells still vastly outnumber other reports. However, current advances in FCM combined with a new generation of cellular reporter probes have made this technique suitable for analyzing physiological responses in bacteria. We review how FCM has been applied to characterize distinct physiological conditions in bacteria including responses to antibiotics and other cytotoxic chemicals and physical factors, pathogen-host interactions, cell differentiation during biofilm formation, and the mechanisms governing development pathways such as sporulation. Since FCM is suitable for performing studies at the single-cell level, we describe how this powerful technique has yielded invaluable information about the heterogeneous distribution of differently and even specialized responding cells and how it may help to provide insights about how cell interaction takes place in complex structures, such as those that prevail in bacterial biofilms.
authors.affiliation: Departamento de Biología, División de Ciencias Naturales y Exactas, Universidad de Guanajuato, Noria Alta S/N, 36050 Guanajuato, GTO, Mexico.
authors.forename: Verónica
authors.identifier: 0000-0001-6614-4240
authors.initials: V
authors.lastname: Ambriz-Aviña
authors.affiliation: Departamento de Biología, División de Ciencias Naturales y Exactas, Universidad de Guanajuato, Noria Alta S/N, 36050 Guanajuato, GTO, Mexico.
authors.forename: Jorge A
authors.initials: JA
authors.lastname: Contreras-Garduño
authors.affiliation: Departamento de Biología, División de Ciencias Naturales y Exactas, Universidad de Guanajuato, Noria Alta S/N, 36050 Guanajuato, GTO, Mexico.
authors.forename: Mario
authors.initials: M
authors.lastname: Pedraza-Reyes
mesh_terms: D018407:Bacterial Physiological Phenomena; D018441:Biofilms; D005434:Flow Cytometry / Q000379:methods*; D054884:Host-Pathogen Interactions; D006801:Humans; D013312:Stress, Physiological