title: Antioxidant and antibacterial activities of exopolysaccharides from Bifidobacterium bifidum WBIN03 and Lactobacillus plantarum R315.
abstract: The objective of this study was to investigate the antioxidant and antibacterial activities of exopolysaccharide (EPS) from Bifidobacterium bifidum WBIN03 (B-EPS) and Lactobacillus plantarum R315 (L-EPS). The 1,1-diphenyl-2-picrylhydrazyl (DPPH)-radical scavenging, hydroxyl radical-scavenging, and superoxide radical-scavenging abilities were measured to evaluate antioxidant activity. Inhibition of erythrocyte hemolysis and lipid peroxidation was also measured. Both B-EPS and L-EPS had strong scavenging ability against DPPH and superoxide radicals at high concentration. The inhibitory effect of B-EPS on erythrocyte hemolysis was stronger than that of L-EPS in a concentration range from 0.30 to 1.00 mg/mL, whereas the hydroxyl scavenging ability of L-EPS (39.15 ± 0.58%) was significantly higher than that of 0.15 mg/mL ascorbic acid (24.33 ± 1.17%) and B-EPS (17.89 ± 3.30%) at 0.10 mg/mL. The inhibition of lipid peroxidation of 0.50 mg/mL B-EPS and L-EPS was 13.48 ± 1.74% and 12.43 ± 0.51%, respectively, values lower than that of ascorbic acid at the same concentration (23.20 ± 1.41%). Furthermore, all these abilities were enhanced in a concentration-dependent manner. Agar diffusion assay showed that both EPS exhibited antibacterial activities against tested pathogens such as Cronobacter sakazakii, Escherichia coli, Listeria monocytogenes, Staphyloccocus aureus, Candida albicans, Bacillus cereus, Salmonella typhimurium, and Shigella sonnei at 300 μg/mL. In conclusion, both EPS have antimicrobial and antioxidant activities and could have applications in the food industry.
authors.affiliation: State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, P. R. China; Jiangxi-OAI Joint Research Institute, Nanchang University, Nanchang 330047, P. R. China.
authors.forename: Shengjie
authors.initials: S
authors.lastname: Li
authors.affiliation: State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, P. R. China.
authors.forename: Renhui
authors.initials: R
authors.lastname: Huang
authors.affiliation: Food and Nutritional Science, School of Biological Science, The University of Hong Kong, Pokfulam Road, Hong Kong, China. Electronic address: npshah@hku.hk.
authors.forename: Nagendra P
authors.initials: NP
authors.lastname: Shah
authors.affiliation: State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, P. R. China; Jiangxi-OAI Joint Research Institute, Nanchang University, Nanchang 330047, P. R. China.
authors.forename: Xueying
authors.initials: X
authors.lastname: Tao
authors.affiliation: State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, P. R. China; Jiangxi-OAI Joint Research Institute, Nanchang University, Nanchang 330047, P. R. China.
authors.forename: Yonghua
authors.initials: Y
authors.lastname: Xiong
authors.affiliation: State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, P. R. China; Jiangxi-OAI Joint Research Institute, Nanchang University, Nanchang 330047, P. R. China. Electronic address: weihua114@live.cn.
authors.forename: Hua
authors.initials: H
authors.lastname: Wei
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D000890:Anti-Infective Agents; D000975:Antioxidants / Q000494:pharmacology*; D001205:Ascorbic Acid / Q000494:pharmacology; D001644:Bifidobacterium / Q000737:chemistry*; D001713:Biphenyl Compounds; D048191:Lactiplantibacillus plantarum / Q000737:chemistry*; D015227:Lipid Peroxidation / Q000187:drug effects; D010084:Oxidation-Reduction; D010851:Picrates; D011135:Polysaccharides, Bacterial / Q000494:pharmacology*
chemical_list: D000900:Anti-Bacterial Agents; D000890:Anti-Infective Agents; D000975:Antioxidants; D001713:Biphenyl Compounds; D010851:Picrates; D011135:Polysaccharides, Bacterial; C004931:1,1-diphenyl-2-picrylhydrazyl; D001205:Ascorbic Acid
keywords: Bifidobacterium bifidum; Lactobacillus plantarum; antioxidative and antibacterial activity; exopolysaccharide