title: Mass-transfer properties of insulin on core-shell and fully porous stationary phases.
abstract: The mass-transfer properties of three superficially-porous packing materials, with 2.6 and 3.6μm particle and 1.9, 2.6, and 3.2μm inner core diameter, respectively, were investigated and compared with those of fully porous packings with similar particle properties. Several sources of band spreading in the chromatographic bed have been identified and studied according to the general rate model of chromatography. Besides the axial dispersion in the stream of the mobile phase, and the external mass transfer resistance, the intraparticle diffusion was studied in depth. The first absolute and the second central moments of the peaks of human insulin, over a wide range of mobile phase velocities were measured and used for the calculation of the mass-transfer coefficients. The experimental data were also analyzed using the stochastic or molecular dynamic model of Giddings and Eyring. The dissimilarities of the mass-transfer observed in the different columns were identified and evaluated.
authors.affiliation: MTA-PTE Molecular Interactions in Separation Science Research Group, Ifjúság útja 6, H-7624 Pécs, Hungary.
authors.forename: Nándor
authors.initials: N
authors.lastname: Lambert
authors.affiliation: Department of Analytical and Environmental Chemistry and Szentágothai Research Center, University of Pécs, Ifjúságútja 6, H-7624 Pécs, Hungary.
authors.forename: Ibolya
authors.initials: I
authors.lastname: Kiss
authors.affiliation: MTA-PTE Molecular Interactions in Separation Science Research Group, Ifjúság útja 6, H-7624 Pécs, Hungary; Department of Analytical and Environmental Chemistry and Szentágothai Research Center, University of Pécs, Ifjúságútja 6, H-7624 Pécs, Hungary. Electronic address: felinger@ttk.pte.hu.
authors.forename: Attila
authors.initials: A
authors.lastname: Felinger
mesh_terms: D002853:Chromatography, Liquid / Q000295:instrumentation*; D004058:Diffusion; D006801:Humans; D007328:Insulin / Q000737:chemistry*; D008962:Models, Theoretical; D008970:Molecular Weight; D016062:Porosity
chemical_list: D007328:Insulin
keywords: Core–shell particles; General rate model; Insulin; Mass transfer; Stochastic theory