title: A highly specific probe for sensing hydrogen sulfide in live cells based on copper-initiated fluorogen with aggregation-induced emission characteristics.
abstract: Here we reported the first fluorescent probe with aggregation-induced emission characteristics, namely AIE-S, for the detection of hydrogen sulfide (H2S) in live cells. The detection system is selective for complicated biological application and the response is fast enough to complete within seconds. Moreover, the probe exhibits the unique advantage of being immune to aggregation-caused quenching which is a detrimental phenomenon limiting the application of most current available H2S fluorescent probes. The detection mechanism was investigated and postulated to be S(2-) initiated de-coordination and thereafter aggregation of the AIE-S complex.
authors.affiliation: 1. ZJU-ENS Joint Laboratory of Medicinal Chemistry, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Li
authors.affiliation: 2. The Key Laboratory of Advanced Textile Materials and Manufacturing Technology, Department of Chemistry, Zhejiang Sci-Tech University, Hangzhou, 310018, China.
authors.forename: Chengyu
authors.initials: C
authors.lastname: Yang
authors.affiliation: 2. The Key Laboratory of Advanced Textile Materials and Manufacturing Technology, Department of Chemistry, Zhejiang Sci-Tech University, Hangzhou, 310018, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Wu
authors.affiliation: 1. ZJU-ENS Joint Laboratory of Medicinal Chemistry, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China.
authors.forename: Yongzhou
authors.initials: Y
authors.lastname: Hu
authors.affiliation: 2. The Key Laboratory of Advanced Textile Materials and Manufacturing Technology, Department of Chemistry, Zhejiang Sci-Tech University, Hangzhou, 310018, China.
authors.forename: Yifeng
authors.initials: Y
authors.lastname: Han
authors.affiliation: 3. Division of Nuclear Medicine and Molecular Imaging, Massachusetts General Hospital and Department of Radiology, Harvard Medical School, 55 Fruit St., Boston, MA 02114, USA.
authors.forename: Steven H
authors.initials: SH
authors.lastname: Liang
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D002477:Cells / Q000737:chemistry* / Q000378:metabolism; D003300:Copper / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D006862:Hydrogen Sulfide / Q000032:analysis* / Q000378:metabolism
chemical_list: D005456:Fluorescent Dyes; D003300:Copper; D006862:Hydrogen Sulfide
keywords: aggregation-induced emission; cell imaging; fluorescent probe; hydrogen sulfide