title: MicroRNA-217 regulates WASF3 expression and suppresses tumor growth and metastasis in osteosarcoma.
abstract: Osteosarcoma is the most common type of primary tumor of bone which mainly affects adolescents and young adults. Osteosarcoma causes large number of deaths because of its complex pathogenesis and resistance to conventional treatment. MicroRNAs are a class of small noncoding RNAs that function as critical gene regulators through targeting mRNAs, causing translational repression or degradation. In this study, we showed that miR-217 was down-regulated in osteosarcoma cell lines and tissues in comparison to that in normal bone cells or tissues. Meanwhile, the lower level of miR-217 was associated with metastasis in clinical osteosarcoma patients. Furthermore, we found that overexpession of miR-217 markedly suppressed cell proliferation, migration, and invasion of osteosarcoma cells. Conversely, the inhibition of miR-217 expression significantly accelerated the cell proliferation, migration, and invasion. Moreover, we identified WASF3 as a novel functional downstream target of miR-217. The ectopic expression of WASF3 can partially reverse the inhibition of cell proliferation and invasion caused by miR-217. Take together, our results demonstrate that miR-217 functions as a tumor-suppressive miRNA and inhibits the osteosarcoma tumorigenesis through targeting WASF3.
authors.affiliation: Department of Anatomy, Qiqihar Medical School, Qiqihar, Heilongjiang, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Shen
authors.affiliation: Department of Orthopedics, The Fourth Hospital of Harbin Medical University, Harbin, Heilongjiang, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Wang
authors.affiliation: Department of TCM, Jilin Province Tumor Hospital, Changchun, Jilin Province, China.
authors.forename: Jili
authors.initials: J
authors.lastname: Yang
authors.affiliation: Department of Orthopedics, The First Affiliated Hospital of Jiamusi University, Jiamusi, Heilongjiang, China.
authors.forename: Xiaotao
authors.initials: X
authors.lastname: Li
mesh_terms: D020413:3' Untranslated Regions; D001483:Base Sequence; D001665:Binding Sites; D001859:Bone Neoplasms / Q000235:genetics* / Q000473:pathology*; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation; D015536:Down-Regulation; D015870:Gene Expression; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D035683:MicroRNAs / Q000737:chemistry / Q000235:genetics*; D009362:Neoplasm Metastasis; D012516:Osteosarcoma / Q000235:genetics* / Q000473:pathology*; D012333:RNA, Messenger / Q000737:chemistry / Q000235:genetics; D047368:Tumor Burden; D051300:Wiskott-Aldrich Syndrome Protein Family / Q000737:chemistry / Q000235:genetics*
chemical_list: D020413:3' Untranslated Regions; C553315:MIRN217 microRNA, human; D035683:MicroRNAs; D012333:RNA, Messenger; D051300:Wiskott-Aldrich Syndrome Protein Family