title: Cell type specific alterations in interchromosomal networks across the cell cycle.
abstract: The interchromosomal organization of a subset of human chromosomes (#1, 4, 11, 12, 16, 17, and 18) was examined in G1 and S phase of human WI38 lung fibroblast and MCF10A breast epithelial cells. Radial positioning of the chromosome territories (CTs) was independent of gene density, but size dependent. While no changes in radial positioning during the cell cycle were detected, there were stage-specific differences between cell types. Each CT was in close proximity (interaction) with a similar number of other CT except the gene rich CT17 which had significantly more interactions. Furthermore, CT17 was a member of the highest pairwise CT combinations with multiple interactions. Major differences were detected in the pairwise interaction profiles of MCF10A versus WI38 including cell cycle alterations from G1 to S. These alterations in interaction profiles were subdivided into five types: overall increase, overall decrease, switching from 1 to ≥2 interactions, vice versa, or no change. A global data mining program termed the chromatic median determined the most probable overall association network for the entire subset of CT. This probabilistic interchromosomal network was nearly completely different between the two cell lines. It was also strikingly altered across the cell cycle in MCF10A, but only slightly in WI38. We conclude that CT undergo multiple and preferred interactions with other CT in the nucleus and form preferred -albeit probabilistic- interchromosomal networks. This network of interactions is altered across the cell cycle and between cell types. It is intriguing to consider the relationship of these alterations to the corresponding changes in the gene expression program across the cell cycle and in different cell types.
authors.affiliation: Department of Biological Sciences, University at Buffalo, State University of New York, Buffalo, New York, United States of America.
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: Fritz
authors.affiliation: Department of Computer Science and Engineering, University at Buffalo, State University of New York, Buffalo, New York, United States of America.
authors.forename: Branislav
authors.initials: B
authors.lastname: Stojkovic
authors.affiliation: Department of Computer Science and Engineering, University at Buffalo, State University of New York, Buffalo, New York, United States of America.
authors.forename: Hu
authors.initials: H
authors.lastname: Ding
authors.affiliation: Department of Computer Science and Engineering, University at Buffalo, State University of New York, Buffalo, New York, United States of America.
authors.forename: Jinhui
authors.initials: J
authors.lastname: Xu
authors.affiliation: Department of Computer Sciences, Fayetteville State University, Fayetteville, North Carolina, United States of America.
authors.forename: Sambit
authors.initials: S
authors.lastname: Bhattacharya
authors.affiliation: Department of Biological Sciences, University at Buffalo, State University of New York, Buffalo, New York, United States of America.
authors.forename: Ronald
authors.initials: R
authors.lastname: Berezney
mesh_terms: D002453:Cell Cycle / Q000235:genetics*; D002460:Cell Line; D002877:Chromosomes, Human / Q000235:genetics*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008957:Models, Genetic; D015233:Models, Statistical