title: 7-Nitroindazole enhances c-Fos expression in spinal neurons in rats realizing operant movements.
abstract: The expression of c-Fos and NADPH-diaphorase reactivity (NADPH-dr) in the cervical spinal cord was studied in adult male Wistar rats that realized operant reflexes after inhibition of neuronal nitric oxide synthase. Fos-immunoreactive neurons were visualized immunohistochemically in the C6/C7 spinal segments in the control, realized operant movements animals, and/or 7-nitroindazole (7-NI) injected rats. The mean numbers of immunoreactive interneurons and motoneurons (per section) were significantly greater in the Nucleus proprius (+240%) and motor nuclei (+600%) in rats of the 7-NI-pretreated and operant reflex realized group than in the isolated operant reflex realized group. Our study showed intensive staining of NADPH-dr axon terminals on the somata and initial parts of dendrites of motoneurons in experimental rats when the disodium salt of malic acid was added to the staining solution. Suppression of NO release is associated with potentiation of neuronal activation induced by descending supraspinal and proprioceptive signaling within the spinal cord.
authors.affiliation: Department of Brain Physiology, State Key Laboratory of Molecular and Cellular Biology, Bogomoletz Institute of Physiology, National Academy of Sciences, Bogomoletz Str. 4, Kiev 01024, Ukraine. Electronic address: elma4@ukr.net.
authors.forename: Yelena P
authors.initials: YP
authors.lastname: Man'kovskaya
authors.affiliation: Department of Brain Physiology, State Key Laboratory of Molecular and Cellular Biology, Bogomoletz Institute of Physiology, National Academy of Sciences, Bogomoletz Str. 4, Kiev 01024, Ukraine.
authors.forename: Vladimir A
authors.initials: VA
authors.lastname: Maisky
authors.affiliation: Laboratory of Experimental Neurophysiology, Pirogov National Medical University, Ministry of Public Health of Ukraine, Pirogov Str. 56, Vinnitsa 21018, Ukraine.
authors.forename: Oleh V
authors.initials: OV
authors.lastname: Vlasenko
authors.affiliation: Department of Movement Physiology, Bogomoletz Institute of Physiology, National Academy of Sciences, Bogomoletz Str. 4, Kiev 01024, Ukraine.
authors.forename: Andriy V
authors.initials: AV
authors.lastname: Maznychenko
mesh_terms: D000818:Animals; D007191:Indazoles / Q000494:pharmacology*; D008297:Male; D009252:NADPH Dehydrogenase / Q000378:metabolism; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism*; D016760:Proto-Oncogene Proteins c-fos / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D013116:Spinal Cord / Q000187:drug effects* / Q000378:metabolism*
chemical_list: D007191:Indazoles; D016760:Proto-Oncogene Proteins c-fos; D009252:NADPH Dehydrogenase; C080122:7-nitroindazole
keywords: 7-Nitroindazole; Nitric oxide; Operant reflex; Rat; c-Fos expression