title: Increased activity of cell membrane-associated prothrombinase, fibrinogen-like protein 2, in peripheral blood mononuclear cells of B-cell lymphoma patients.
abstract: Fibrinogen-like protein 2, FGL-2, was reported to be overexpressed in various cancer tissues, where it acts as a transmembrane prothrombinase. This study aims to determine the prothrombinase activity of FGL-2 in peripheral blood mononuclear cells (PBMC) of patients with B-cell lymphoma. FGL-2 activity was determined in patients with B-cell lymphoma (n = 53), and healthy controls (n = 145). FGL-2 activity in patients at diagnosis increased 3 ± 0.3 fold (p < 0.001). Sensitivity and specificity of the test was established at 73.6% and 80.7%, respectively, using a cutoff of 150% activity over control. Moreover, FGL-2 activity in 10 of 11 patients in remission decreased by 76%. In contrast, no significant difference was observed in expression levels of fgl-2 gene in patients and controls. Taken together, our study indicates that FGL-2 prothrombinase activity in PBMC of lymphoma patients is increased in active disease and normalizes during remission, thus being a potential marker for follow up of lymphoma patients.
authors.affiliation: Hemato-Oncology Laboratory, Felsenstein Medical Research Center, Rabin Medical Center, Petach Tikva, Israel, affiliated with Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel; Hematology Laboratory, Rabin Medical Center, Petach Tikva, Israel.
authors.forename: Esther
authors.initials: E
authors.lastname: Rabizadeh
authors.affiliation: Hemato-Oncology Laboratory, Felsenstein Medical Research Center, Rabin Medical Center, Petach Tikva, Israel, affiliated with Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel.
authors.forename: Izhack
authors.initials: I
authors.lastname: Cherny
authors.affiliation: Thrombosis and Hemostasis Unit, Hematology Institute, Rabin Medical Center, Petach Tikva, Israel, affiliated with Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel.
authors.forename: Ofir
authors.initials: O
authors.lastname: Wolach
authors.affiliation: Hemato-Oncology Laboratory, Felsenstein Medical Research Center, Rabin Medical Center, Petach Tikva, Israel, affiliated with Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel; Thrombosis and Hemostasis Unit, Hematology Institute, Rabin Medical Center, Petach Tikva, Israel, affiliated with Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel.
authors.forename: Shany
authors.initials: S
authors.lastname: Sherman
authors.affiliation: Hemato-Oncology Laboratory, Felsenstein Medical Research Center, Rabin Medical Center, Petach Tikva, Israel, affiliated with Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel.
authors.forename: Natalia
authors.initials: N
authors.lastname: Binkovski
authors.affiliation: Occupational Health Clinic, Rabin Medical Center, Petach Tikva, Israel.
authors.forename: Alon
authors.initials: A
authors.lastname: Peretz
authors.affiliation: Hemato-Oncology Laboratory, Felsenstein Medical Research Center, Rabin Medical Center, Petach Tikva, Israel, affiliated with Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel.
authors.forename: Doron
authors.initials: D
authors.lastname: Lederfein
authors.affiliation: Thrombosis and Hemostasis Unit, Hematology Institute, Rabin Medical Center, Petach Tikva, Israel, affiliated with Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel.
authors.forename: Aida
authors.initials: A
authors.lastname: Inbal
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015415:Biomarkers / Q000378:metabolism; D002462:Cell Membrane / Q000378:metabolism; D005260:Female; D005340:Fibrinogen / Q000378:metabolism*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D016393:Lymphoma, B-Cell / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D013925:Thromboplastin / Q000378:metabolism*
chemical_list: D015415:Biomarkers; C095202:FGL2 protein, human; D005340:Fibrinogen; D013925:Thromboplastin