title: Enhancement of skin wound healing with decellularized scaffolds loaded with hyaluronic acid and epidermal growth factor.
abstract: Current therapy for skin wound healing still relies on skin transplantation. Many studies were done to try to find out ways to replace skin transplantation, but there is still no effective alternative therapy. In this study, decellularized scaffolds were prepared from pig peritoneum by a series of physical and chemical treatments, and scaffolds loaded with hyaluronic acid (HA) and epidermal growth factor (EGF) were tested for their effect on wound healing. MTT assay showed that EGF increased NIH3T3 cell viability and confirmed that EGF used in this study was biologically active in vitro. Scanning electron microscope (SEM) showed that HA stably attached to scaffolds even after soaking in PBS for 48 h. ELISA assay showed that HA increased the adsorption of EGF to scaffolds and sustained the release of EGF from scaffolds. Animal study showed that the wounds covered with scaffolds containing HA and EGF recovered best among all 4 groups and had wound healing rates of 49.86%, 70.94% and 87.41% respectively for days 10, 15 and 20 post-surgery compared to scaffolds alone with wound healing rates of 29.26%, 42.80% and 70.14%. In addition, the wounds covered with scaffolds containing EGF alone were smaller than no EGF scaffolds on days 10, 15 and 20 post-surgery. Hematoxylin-Eosin (HE) staining confirmed these results by showing that on days 10, 15 and 20 post-surgery, the thicker epidermis and dermis layers were observed in the wounds covered with scaffolds containing HA and EGF than scaffolds alone. In addition, the thicker epidermis and dermis layers were also observed in the wounds covered with scaffolds containing EGF than scaffolds alone. Skin appendages were observed on day 20 only in the wound covered with scaffolds containing HA and EGF. These results demonstrate that the scaffolds containing HA and EGF can enhance wound healing.
authors.affiliation: Institute of Biomedicine, National Engineering Research Center of Genetic Medicine, Key Lab for Genetic Medicine of Guangdong Province, Jinan University, Guangzhou 510632, China.
authors.forename: Zhongchun
authors.initials: Z
authors.lastname: Su
authors.affiliation: Institute of Biomedicine, National Engineering Research Center of Genetic Medicine, Key Lab for Genetic Medicine of Guangdong Province, Jinan University, Guangzhou 510632, China.
authors.forename: Huan
authors.initials: H
authors.lastname: Ma
authors.affiliation: Institute of Biomedicine, National Engineering Research Center of Genetic Medicine, Key Lab for Genetic Medicine of Guangdong Province, Jinan University, Guangzhou 510632, China.
authors.forename: Zhengzheng
authors.initials: Z
authors.lastname: Wu
authors.affiliation: Department of Hematology, The First Affiliated Hospital, Jinan University, Guangzhou 510632, China.
authors.forename: Huilan
authors.initials: H
authors.lastname: Zeng
authors.affiliation: Department of Bone, The First Affiliated Hospital, Jinan University, Guangzhou 510632, China.
authors.forename: Zhizhong
authors.initials: Z
authors.lastname: Li
authors.affiliation: Department of Physiology, School of Medicine, Jinan University, Guangzhou 510632, China.
authors.forename: Yuechun
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Physiology, School of Medicine, Jinan University, Guangzhou 510632, China.
authors.forename: Gexiu
authors.initials: G
authors.lastname: Liu
authors.affiliation: Grandhope Biotech Co., Ltd., Building D, #408, Guangzhou International Business Incubator, Guangzhou Science Park, Guangzhou 510663, Guangdong, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Xu
authors.affiliation: Grandhope Biotech Co., Ltd., Building D, #408, Guangzhou International Business Incubator, Guangzhou Science Park, Guangzhou 510663, Guangdong, China.
authors.forename: Yongliang
authors.initials: Y
authors.lastname: Lin
authors.affiliation: Grandhope Biotech Co., Ltd., Building D, #408, Guangzhou International Business Incubator, Guangzhou Science Park, Guangzhou 510663, Guangdong, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Zhang
authors.affiliation: Institute of Biomedicine, National Engineering Research Center of Genetic Medicine, Key Lab for Genetic Medicine of Guangdong Province, Jinan University, Guangzhou 510632, China. Electronic address: wei70@hotmail.com.
authors.forename: Xing
authors.initials: X
authors.lastname: Wei
mesh_terms: D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D004815:Epidermal Growth Factor / Q000737:chemistry / Q000494:pharmacology*; D006820:Hyaluronic Acid / Q000737:chemistry / Q000494:pharmacology*; D051379:Mice; D008855:Microscopy, Electron, Scanning; D041681:NIH 3T3 Cells; D012867:Skin / Q000187:drug effects* / Q000293:injuries; D016038:Skin Transplantation; D013552:Swine; D054457:Tissue Scaffolds / Q000737:chemistry*; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D004815:Epidermal Growth Factor; D006820:Hyaluronic Acid
keywords: Decellularized scaffolds; Growth factors; Skin repair