title: Characterization of the lipopolysaccharide produced by Pasteurella multocida serovars 6, 7 and 16: identification of lipopolysaccharide genotypes L4 and L8.
abstract: Pasteurella multocida is an important veterinary pathogen that produces a wide range of lipopolysaccharide (LPS) structures, many of which mimic host glycoproteins. In this study, we complete our analysis of the LPS produced by the P. multocida Heddleston serovars by reporting the LPS structure and the LPS outer core biosynthesis loci of the type strains representing Heddleston serovars 6, 7 and 16. Genetic analysis revealed that the type strains representing serovars 6 and 7 share the same LPS outer core biosynthesis locus which we have designated LPS genotype L4. Comparative bioinformatic analysis revealed that although the serovar 16 type strain contained a different LPS locus, L8, there was a significant degree of nucleotide identity between the L4 and L8 loci. Structural analysis revealed that the LPS glycoforms produced by the L4 and L8 strains all contained the highly conserved inner core produced by all other P. multocida strains examined to date. The residues within the LPS outer core produced by the L4 and L8 strains were either Gal or derivatives of Gal; unlike all other P. multocida Heddleston type strains examined there are no heptosyltransferases encoded in the L4 and L8 outer core biosynthesis loci. The structure of the L4 LPS outer core produced by the serovar 6 type strain consisted of β-Gal-(1-3)-β-N-acetylgalactosamine (GalNAc)-(1-4)-β-GalNAc3OAc-(1-4)-α-GalNAc3OAc-(1-3)-β-Gal, whereas the serovar 7 type strain produced a highly truncated LPS outer core containing only a single β-Gal residue. The structure of the L8 LPS outer core produced by the serovar 16 type strain consisted of β-Gal-(1-3)-β-GalNAc-(1-4)-(α-GalNAc-(1-3)-)-α-GalNAc.
authors.affiliation: Department of Microbiology, Australian Research Council Centre of Excellence in Structural and Functional Microbial Genomics marina.harper@monash.edu.
authors.forename: Marina
authors.initials: M
authors.lastname: Harper
authors.affiliation: Vaccine Program, Human Health Therapeutics Portfolio, National Research Council, Ottawa, ON, Canada K1A 0R6.
authors.forename: Frank
authors.initials: F
authors.lastname: St Michael
authors.affiliation: Department of Microbiology, Australian Research Council Centre of Excellence in Structural and Functional Microbial Genomics.
authors.forename: Jason A
authors.initials: JA
authors.lastname: Steen
authors.affiliation: Department of Microbiology, Monash University, Building 76, Clayton, VIC 3800, Australia.
authors.forename: Marietta
authors.initials: M
authors.lastname: John
authors.affiliation: Department of Microbiology, Monash University, Building 76, Clayton, VIC 3800, Australia.
authors.forename: Amy
authors.initials: A
authors.lastname: Wright
authors.affiliation: Vaccine Program, Human Health Therapeutics Portfolio, National Research Council, Ottawa, ON, Canada K1A 0R6.
authors.forename: Lieke
authors.initials: L
authors.lastname: van Dorsten
authors.affiliation: Vaccine Program, Human Health Therapeutics Portfolio, National Research Council, Ottawa, ON, Canada K1A 0R6.
authors.forename: Evgeny
authors.initials: E
authors.lastname: Vinogradov
authors.affiliation: Department of Microbiology, Australian Research Council Centre of Excellence in Structural and Functional Microbial Genomics.
authors.forename: Ben
authors.initials: B
authors.lastname: Adler
authors.affiliation: Vaccine Program, Human Health Therapeutics Portfolio, National Research Council, Ottawa, ON, Canada K1A 0R6.
authors.forename: Andrew D
authors.initials: AD
authors.lastname: Cox
authors.affiliation: Department of Microbiology, Australian Research Council Centre of Excellence in Structural and Functional Microbial Genomics Department of Microbiology, Monash University, Building 76, Clayton, VIC 3800, Australia.
authors.forename: John D
authors.initials: JD
authors.lastname: Boyce
mesh_terms: D020563:Gammaproteobacteria / Q000235:genetics* / Q000378:metabolism; D005838:Genotype; D008070:Lipopolysaccharides / Q000096:biosynthesis / Q000737:chemistry*; D065288:Serogroup
chemical_list: D008070:Lipopolysaccharides
keywords: LPS; Pasteurella multocida; core oligosaccharide; genetics; structure