title: Ischemia postconditioning preventing lung ischemia-reperfusion injury.
abstract: OBJECTIVE
  This study evaluates the inhibitory effect of IPO against ischemia reperfusion (I/R) induced lung injury in rats.
  
  
  METHODS
  Anesthetized and mechanically ventilated adult Sprague-Dawley rats were randomly assigned to one of the following groups (n=12 each): the sham operated control group, the ischemia-reperfusion (IR) group (30min of left-lung ischemia and 24h of reperfusion), the IPO group (three successive cycles of 30-s reperfusion per 30-s occlusion before restoring full perfusion), and the dexamethasone plus IPO group (rats were injected with dexamethasone (3mg/kg·day(-1)) 10min prior to the experiment and the rest of the procedures were the same as the IPO group). Lung injury was assessed by wet-to-dry lung weight ratio and tissue apoptosis and biochemical changes.
  
  
  RESULTS
  Lung ischemia-reperfusion increased lung MDA production, serum proinflammatory cytokine count, and MPO activity and reduced antioxidant enzyme activities (all p<0.05 I/R versus sham), accompanied with a compensatory increase in caspase-3, bax, Fas, FasL proteins and a decrease in Bcl-2 protein. Plasma levels of TNF-α, IL-6, and IL-1β were increased in the I/R group (all p<0.05 versus sham). IPO attenuated or prevented all the above changes. Treatment with dexamethasone enhanced all the protective effects of postconditioning.
  
  
  CONCLUSIONS
  Postconditioning obviously inhibits I/R induced lung injury by its antioxidant, anti-inflammatory and anti-apoptosis activities.
authors.affiliation: 3rd Department of Internal Medicine, Integrated Chinese and Western Medicine Hospital of Taizhou, Zhejiang Province 317523, China. Electronic address: cjgktxyy@sohu.com.
authors.forename: Qi-Feng
authors.initials: QF
authors.lastname: Cao
authors.affiliation: 3rd Department of Internal Medicine, Integrated Chinese and Western Medicine Hospital of Taizhou, Zhejiang Province 317523, China.
authors.forename: Mei-Jun
authors.initials: MJ
authors.lastname: Qu
authors.affiliation: 3rd Department of Internal Medicine, Integrated Chinese and Western Medicine Hospital of Taizhou, Zhejiang Province 317523, China.
authors.forename: Wei-Qin
authors.initials: WQ
authors.lastname: Yang
authors.affiliation: 3rd Department of Internal Medicine, Integrated Chinese and Western Medicine Hospital of Taizhou, Zhejiang Province 317523, China.
authors.forename: Dan-Ping
authors.initials: DP
authors.lastname: Wang
authors.affiliation: 3rd Department of Internal Medicine, Integrated Chinese and Western Medicine Hospital of Taizhou, Zhejiang Province 317523, China.
authors.forename: Ming-Hui
authors.initials: MH
authors.lastname: Zhang
authors.affiliation: 3rd Department of Internal Medicine, Integrated Chinese and Western Medicine Hospital of Taizhou, Zhejiang Province 317523, China.
authors.forename: Song-Bo
authors.initials: SB
authors.lastname: Di
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D017209:Apoptosis; D053148:Caspase 3 / Q000378:metabolism; D003907:Dexamethasone / Q000737:chemistry; D053222:Fas Ligand Protein / Q000378:metabolism; D016166:Free Radical Scavengers; D007249:Inflammation; D053583:Interleukin-1beta / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D057775:Ischemic Postconditioning; D008168:Lung / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D009195:Peroxidase / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000517:prevention & control*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D051028:bcl-2-Associated X Protein / Q000378:metabolism; D019014:fas Receptor / Q000378:metabolism
chemical_list: D000975:Antioxidants; C494828:Bax protein, rat; D053222:Fas Ligand Protein; C491898:Fas protein, rat; C505662:Faslg protein, rat; D016166:Free Radical Scavengers; D053583:Interleukin-1beta; D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha; D051028:bcl-2-Associated X Protein; D019014:fas Receptor; D008315:Malondialdehyde; D003907:Dexamethasone; D009195:Peroxidase; C505410:Casp3 protein, rat; D053148:Caspase 3
keywords: Antioxidant; Ischemic postconditioning; Lung ischemia reperfusion