title: Comparison between Oligoryzomys nigripes and O. flavescens by RAPD and genetic diversity in O. nigripes (Rodentia, Cricetidae).
abstract: The genus of Oligoryzomys includes species of small size, morphologically similar, which may impede taxonomic identification, mainly between O. flavescens (Waterhouse, 1837) and O. nigripes (Olfers, 1818). The main objective of this work was to investigate whether the RAPD markers are capable of genetically differentiating the specimens O. nigripes and O. flavescens, coming from Rio Grande do Sul (RS) and Santa Catarina (SC) states, and also to estimate the genetic variability among populations of O. nigripes, with the Uruguay River as a geographical barrier. For this purpose, samples were collected in fragments of forests situated in the North of RS, at FLONA (Floresta Nacional de Passo Fundo) and in fragments from SC, close to the Uruguay River. The karyotyping of two samples for each species was carried out and compared using the RAPD technique together with non- karyotyped individuals. Samples of O. nigripes presented 2n = 62; NA = 82, with submetacentric arms on the largest chromosomes, while samples of O. flavescens showed 2n = 64; NA = 66, with the largest chromosomes presenting acrocentric morphology, making such a result the main difference between the species. The analysis was able to detect two distinct groups, being the first one with karyotyped O. flavescens and the second with karyotyped O. nigripes. Identification afforded 211 loci, among them 181 (85.78%) polymorphic. The Jaccard similarity coefficient was in the range of 0.45 to 0.87. The UPGMA and Main Coordinate Analysis techniques demonstrated the existence of heterogeneous genetics among populations, but did not separate them completely in terms of geographical standards, and they are not influenced by the Uruguay River, which did not act as an efficient barrier.
authors.affiliation: Universidade Federal da Fronteira Sul - UFFS, Erechim, RS, Brazil.
authors.forename: A J
authors.initials: AJ
authors.lastname: Mossi
authors.affiliation: Geonature, Erechim, RS, Brazil.
authors.forename: V J
authors.initials: VJ
authors.lastname: Coppini
authors.affiliation: Universidade Regional Integrada do Alto Uruguai e das Missões - URI, Campus de Erechim, Erechim, RS, Brazil.
authors.forename: L B
authors.initials: LB
authors.lastname: Slaviero
authors.affiliation: Universidade Regional Integrada do Alto Uruguai e das Missões - URI, Campus de Erechim, Erechim, RS, Brazil.
authors.forename: G B
authors.initials: GB
authors.lastname: Kubiak
authors.affiliation: Departamento de Engenharia Química e de Alimentos, Universidade Federal de Santa Catarina - UFSC, Campus Universitário Reitor João David Ferreira Lima, Florianópolis, SC, Brazil.
authors.forename: L A
authors.initials: LA
authors.lastname: Lerin
authors.affiliation: Departamento de Engenharia Química e de Alimentos, Universidade Federal de Santa Catarina - UFSC, Campus Universitário Reitor João David Ferreira Lima, Florianópolis, SC, Brazil.
authors.forename: J V
authors.initials: JV
authors.lastname: Oliveira
authors.affiliation: Caipora Cooperativa para Conservação da Natureza, Florianópolis, SC, Brazil.
authors.forename: J J
authors.initials: JJ
authors.lastname: Cherem
authors.affiliation: Universidade Regional Integrada do Alto Uruguai e das Missões - URI, Campus de Erechim, Erechim, RS, Brazil.
authors.forename: J R
authors.initials: JR
authors.lastname: Marinho
authors.affiliation: Universidade Regional Integrada do Alto Uruguai e das Missões - URI, Campus de Erechim, Erechim, RS, Brazil.
authors.forename: R L
authors.initials: RL
authors.lastname: Cansian
mesh_terms: D000818:Animals; D005819:Genetic Markers; D014644:Genetic Variation; D007621:Karyotyping; D019105:Random Amplified Polymorphic DNA Technique; D012377:Rodentia / Q000235:genetics; D016205:Sigmodontinae / Q000145:classification* / Q000235:genetics*
chemical_list: D005819:Genetic Markers