title: Expression and significance of transient receptor potential cation channel V5 in articular cartilage cells under exercise loads.
abstract: The expression of transient receptor potential cation channel V5 (TRPV5) in articular cartilage cells under normal and exercise loading conditions was measured, and the clinical significance, in order to define its role in the formation of articular cartilage tissues was analyzed. In normal and osteoarthritis (OA) Sprague Dawley rats the severity of injury was observed, TRPV5 expression was measured by immunohistochemistry following exercise loading, and its association with clinical pathological characteristics (including articular lesions) was analyzed. The results of the immunohistochemical assay showed that the percentage of positive expression areas in the normal articular cartilage and loading articular cartilage groups was 34.3±5.8 and 18.1±4.9%, respectively. In the OA articular cartilage and OA loading articular cartilage groups, the positive expression areas were 13.17±4.2 and 6.4±2.7%, respectively. There was a statistically significant difference in TRPV5 expression levels between the normal articular cartilage and loading articular cartilage groups, and between the OA articular cartilage and OA loading articular cartilage groups (P<0.001). TRPV5 is expressed in all bone cartilage tissues and its expression level depends on the load of the bone and joint. Therefore, this indicates that TRPV5 may play a role in the formation and development processes of cartilage tissues.
authors.affiliation: Department of Orthopaedics, Shengjing Hospital, China Medical University, Shenyang, Liaoning 110004, P.R. China.
authors.forename: Xiaonan
authors.initials: X
authors.lastname: Zhou
authors.affiliation: Research Center, Shengjing Hospital, China Medical University, Shenyang, Liaoning 110004, P.R. China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.affiliation: Research Center, Shengjing Hospital, China Medical University, Shenyang, Liaoning 110004, P.R. China.
authors.forename: Jianing
authors.initials: J
authors.lastname: Miao
authors.affiliation: Department of Orthopaedics, Shengjing Hospital, China Medical University, Shenyang, Liaoning 110004, P.R. China.
authors.forename: Lunhao
authors.initials: L
authors.lastname: Bai
keywords: cartilage cell; exercise load; immunohistochemistry; osteoarthritis; transient receptor potential cation channel V5; transient receptor potential channel