title: Dynamic contact angle analysis of protein adsorption on polysaccharide multilayer's films for biomaterial reendothelialization.
abstract: Atherosclerosis is a major cardiovascular disease. One of the side effects is restenosis. The aim of this work was to study the coating of stents by dextran derivates based polyelectrolyte's multilayer (PEM) films in order to increase endothelialization of injured arterial wall after stent implantation. Films were composed with diethylaminoethyl dextran (DEAE) as polycation and dextran sulphate (DS) as polyanion. One film was composed with 4 bilayers of (DEAE-DS)4 and was labeled D-. The other film was the same as D- but with an added terminal layer of DEAE polycation: (DEAE-DS)4-DEAE (labeled D+). The dynamic adsorption/desorption of proteins on the films were characterized by dynamic contact angle (DCA) and atomic force microscopy (AFM). Human endothelial cell (HUVEC) adhesion and proliferation were quantified and correlated to protein adsorption analyzed by DCA for fibronectin, vitronectin, and bovine serum albumin (BSA). Our results showed that the endothelial cell response was optimal for films composed of DS as external layer. Fibronectin was found to be the only protein to exhibit a reversible change in conformation after desorption test. This behavior was only observed for (DEAE-DS)4 films. (DEAE-DS)4 films could enhance HUVEC proliferation in agreement with fibronectin ability to easily change from conformation.
authors.affiliation: INSERM, U1148, LVTS, Institut Galilée, Université Paris 13, Sorbonne Paris Cité, 93430 Villetaneuse, France.
authors.forename: Safiya
authors.initials: S
authors.lastname: Benni
authors.affiliation: INSERM, U1148, LVTS, Institut Galilée, Université Paris 13, Sorbonne Paris Cité, 93430 Villetaneuse, France.
authors.forename: Thierry
authors.identifier: 0000-0002-9560-3052
authors.initials: T
authors.lastname: Avramoglou
authors.affiliation: INSERM, U1148, LVTS, UFR SMBH, Université Paris 13, Sorbonne Paris Cité, 74 rue Marcel Cachin, 93 000 Bobigny, France.
authors.forename: Hanna
authors.identifier: 0000-0003-3877-4266
authors.initials: H
authors.lastname: Hlawaty
authors.affiliation: INSERM, U1148, LVTS, Institut Galilée, Université Paris 13, Sorbonne Paris Cité, 93430 Villetaneuse, France.
authors.forename: Laurence
authors.initials: L
authors.lastname: Mora
mesh_terms: D000327:Adsorption; D000818:Animals; D001672:Biocompatible Materials / Q000494:pharmacology*; D002417:Cattle; D002448:Cell Adhesion / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D003637:DEAE-Dextran / Q000737:chemistry; D016264:Dextran Sulfate / Q000737:chemistry; D005353:Fibronectins / Q000302:isolation & purification; D061307:Human Umbilical Vein Endothelial Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D018625:Microscopy, Atomic Force; D008856:Microscopy, Fluorescence; D011134:Polysaccharides / Q000737:chemistry*; D011506:Proteins / Q000302:isolation & purification*; D012710:Serum Albumin, Bovine / Q000302:isolation & purification; D013500:Surface Tension / Q000187:drug effects; D019096:Vitronectin / Q000302:isolation & purification; D017155:Wettability / Q000187:drug effects
chemical_list: D001672:Biocompatible Materials; D005353:Fibronectins; D011134:Polysaccharides; D011506:Proteins; D019096:Vitronectin; D012710:Serum Albumin, Bovine; D003637:DEAE-Dextran; D016264:Dextran Sulfate