title: Cytotoxicity evaluation of root repair materials in human-cultured periodontal ligament fibroblasts.
abstract: OBJECTIVE
  To evaluate the cytotoxicity of three root repair materials, mineral trioxide aggregate (MTA), Endosequence Root Repair Material and Biodentine in human periodontal ligament fibroblasts.
  
  
  METHODS
  Periodontal ligament fibroblasts were cultured from healthy premolar extracted for orthodontic purpose. Cells in the third passage were used in the study. The cultured fibroblast cells were placed in contact with root repair materials: (a) Biodentine, (b) MTA, (c) Endosequence, (d) control. The effects of these three materials on the viability of Periodontal ligament (PDL) fibroblasts were determined by trypan blue dye assay after 24 hours and 48-hour time period. Cell viability was determined using inverted phase contrast microscope.
  
  
  METHODS
  Cell viability was compared for all the experimental groups with Wilcoxons matched pair test.
  
  
  RESULTS
  At the 24-hour examination period, all the materials showed increased cell viability. At 48-hour time period, there is slight decrease in cell viability. Mineral trioxide aggregate showed statistically significant increase in the cell viability when compared to other root repair materials.
  
  
  CONCLUSIONS
  Mineral trioxide aggregate was shown to be less toxic to periodontal ligament fibroblasts than Endosequence Root Repair Material and Biodentine.
authors.affiliation: Department of Conservative Dentistry and Endodontics, SIBAR Institute of Dental Sciences, Guntur, Andhra Pradesh, India.
authors.forename: Voruganti
authors.initials: V
authors.lastname: Samyuktha
authors.affiliation: Department of Conservative Dentistry and Endodontics, SIBAR Institute of Dental Sciences, Guntur, Andhra Pradesh, India.
authors.forename: Pabbati
authors.initials: P
authors.lastname: Ravikumar
authors.affiliation: Department of Conservative Dentistry and Endodontics, SIBAR Institute of Dental Sciences, Guntur, Andhra Pradesh, India.
authors.forename: Bolla
authors.initials: B
authors.lastname: Nagesh
authors.affiliation: Department of Oral and Maxillofacial Pathology, RAGAS Dental College, Chennai, Tamil Nadu, India.
authors.forename: K
authors.initials: K
authors.lastname: Ranganathan
authors.affiliation: Professor and Head, St. Joseph Dental College, Eluru, Andhra Pradesh, India.
authors.forename: Thumu
authors.initials: T
authors.lastname: Jayaprakash
authors.affiliation: Department of Conservative Dentistry and Endodontics, SIBAR Institute of Dental Sciences, Guntur, Andhra Pradesh, India.
authors.forename: Vemuri
authors.initials: V
authors.lastname: Sayesh
keywords: Biocompatibility; periodontal ligament fibroblasts; root repair material