title: Genetic Instability of Influenza pH1N1 Viruses.
abstract: Here, we report full-length genome sequences of influenza pH1N1 viruses obtained prior to and after propagation in MDCK cells. Paired comparisons of the genomes showed that each strain acquired 1.0 to 18.8 mutations per genome per replication cycle, which corresponds to 0.5 to 5.8 mutations per virus proteome per replication cycle. Our analysis indicates that pH1N1 viruses accumulated adaptive mutations among others in response to propagation in cell culture. These results could be important for vaccine and drug-sensitivity surveillance studies, as well as for vaccine and antiviral drug development programs where cell cultures are used for influenza propagation.
authors.affiliation: The Institute for Molecular Medicine Finland (FIMM), Helsinki, Finland.
authors.forename: Petri
authors.initials: P
authors.lastname: Jalovaara
authors.affiliation: The Institute for Molecular Medicine Finland (FIMM), Helsinki, Finland.
authors.forename: Dmitrii
authors.initials: D
authors.lastname: Bychkov
authors.affiliation: The Institute for Molecular Medicine Finland (FIMM), Helsinki, Finland.
authors.forename: Laura
authors.initials: L
authors.lastname: Ahtiainen
authors.affiliation: Helsinki University Hospital Laboratory, HUSLAB, Helsinki, Finland.
authors.forename: Hannimari
authors.initials: H
authors.lastname: Kallio-Kokko
authors.affiliation: Helsinki University Central Hospital, HUCS, Helsinki, Finland.
authors.forename: Miia
authors.initials: M
authors.lastname: Valkonen
authors.affiliation: Helsinki University Central Hospital, HUCS, Helsinki, Finland.
authors.forename: Anu
authors.initials: A
authors.lastname: Kantele
authors.affiliation: The Institute for Molecular Medicine Finland (FIMM), Helsinki, Finland.
authors.forename: Pirkko
authors.initials: P
authors.lastname: Mattila
authors.affiliation: The Institute for Molecular Medicine Finland (FIMM), Helsinki, Finland.
authors.forename: Henrikki
authors.initials: H
authors.lastname: Almusa
authors.affiliation: The Institute for Molecular Medicine Finland (FIMM), Helsinki, Finland.
authors.forename: Olli
authors.initials: O
authors.lastname: Kallioniemi
authors.affiliation: The Institute for Molecular Medicine Finland (FIMM), Helsinki, Finland denis.kainov@helsinki.fi.
authors.forename: Denis
authors.initials: D
authors.lastname: Kainov