title: Investigation of avian influenza virus in poultry and wild birds due to novel avian-origin influenza A(H10N8) in Nanchang City, China.
abstract: Multiple reassortment events within poultry and wild birds had resulted in the establishment of another novel avian influenza A(H10N8) virus, and finally resulted in human death in Nanchang, China. However, there was a paucity of information on the prevalence of avian influenza virus in poultry and wild birds in Nanchang area. We investigated avian influenza virus in poultry and wild birds from live poultry markets, poultry countyards, delivery vehicles, and wild-bird habitats in Nanchang. We analyzed 1036 samples from wild birds and domestic poultry collected from December 2013 to February 2014. Original biological samples were tested for the presence of avian influenza virus using specific primer and probe sets of H5, H7, H9, H10 and N8 subtypes by real-time RT-PCR. In our analysis, the majority (97.98%) of positive samples were from live poultry markets. Among the poultry samples from chickens and ducks, AIV prevalence was 26.05 and 30.81%, respectively. Mixed infection of different HA subtypes was very common. Additionally, H10 subtypes coexistence with N8 was the most prevalent agent during the emergence of H10N8. This event illustrated a long-term surveillance was so helpful for pandemic preparedness and response.
authors.affiliation: Nanchang Center for Disease Control and Prevention, Nanchang 330038, China.
authors.forename: Xiansheng
authors.initials: X
authors.lastname: Ni
authors.affiliation: Nanchang Center for Disease Control and Prevention, Nanchang 330038, China. Electronic address: he_495380@163.com.
authors.forename: Fenglan
authors.initials: F
authors.lastname: He
authors.affiliation: Nanchang Center for Disease Control and Prevention, Nanchang 330038, China.
authors.forename: Maohong
authors.initials: M
authors.lastname: Hu
authors.affiliation: Nanchang Center for Disease Control and Prevention, Nanchang 330038, China.
authors.forename: Xianfeng
authors.initials: X
authors.lastname: Zhou
authors.affiliation: Nanchang Center for Disease Control and Prevention, Nanchang 330038, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Wang
authors.affiliation: Nanchang Center for Disease Control and Prevention, Nanchang 330038, China.
authors.forename: Changhua
authors.initials: C
authors.lastname: Feng
authors.affiliation: Qingshanhu District Center for Disease Control and Prevention, Nanchang 330038, China.
authors.forename: Yumei
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Xinjian County Center for Disease Control and Prevention, Nanchang 330038, China.
authors.forename: Youxing
authors.initials: Y
authors.lastname: Li
authors.affiliation: Nanchang Center for Disease Control and Prevention, Nanchang 330038, China.
authors.forename: Junling
authors.initials: J
authors.lastname: Tu
authors.affiliation: Nanchang Center for Disease Control and Prevention, Nanchang 330038, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Li
authors.affiliation: Nanchang Center for Disease Control and Prevention, Nanchang 330038, China.
authors.forename: Mingbin
authors.initials: M
authors.lastname: Liu
authors.affiliation: Nanchang Center for Disease Control and Prevention, Nanchang 330038, China.
authors.forename: Haiying
authors.initials: H
authors.lastname: Chen
authors.affiliation: Nanchang Center for Disease Control and Prevention, Nanchang 330038, China. Electronic address: chen903@126.com.
authors.forename: Shengen
authors.initials: S
authors.lastname: Chen
mesh_terms: D000818:Animals; D001717:Birds; D002681:China / Q000453:epidemiology; D062665:Epidemiological Monitoring; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000235:genetics; D065926:Influenza A Virus, H10N8 Subtype / Q000235:genetics / Q000302:isolation & purification*; D005585:Influenza in Birds / Q000453:epidemiology* / Q000821:virology*; D009439:Neuraminidase / Q000235:genetics; D011200:Poultry; D015995:Prevalence; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014764:Viral Proteins / Q000235:genetics
chemical_list: D019267:Hemagglutinin Glycoproteins, Influenza Virus; D014764:Viral Proteins; C487630:NA protein, influenza A virus; D009439:Neuraminidase
keywords: Avian flu; Avian influenza virus; H10N8; H7N9