title: Protective effect of beta-carotene against titanium dioxide nanoparticles induced apoptosis in mouse testicular tissue.
abstract: In this study, the effects of beta-carotene (BC) on testicular germ cell apoptosis arising from titanium dioxide nanoparticles (NTiO2 ) have been evaluated. In NTiO2 -treated mice, expression of apoptotic related genes including Bid, FasL, caspase-3 and p38MAPK was significantly increased. Measurement apoptosis using TUNEL method showed significant increase in apoptotic index of germ cells in NTiO2 -treated mice (P < 0.05). TUNEL assessments showed that the increase of apoptotic index of testicular germ cells in NTiO2 -treated mice was reversed by BC. Beta-carotene pre-treatment could also effectively attenuate the expression of apoptotic related genes. The application of BC may serve as a beneficial medication to protect germ cells against apoptosis induced by nanoparticles and be helpful for male fertility.
authors.affiliation: Cell & Molecular Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.|Department of Anatomical Sciences Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
authors.forename: M
authors.initials: M
authors.lastname: Orazizadeh
authors.affiliation: Cell & Molecular Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
authors.forename: E
authors.initials: E
authors.lastname: Daneshi
authors.affiliation: Cell & Molecular Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.|Department of Anatomical Sciences Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
authors.forename: M
authors.initials: M
authors.lastname: Hashemitmar
authors.affiliation: Cell & Molecular Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
authors.forename: F
authors.initials: F
authors.lastname: Absalan
authors.affiliation: Cell & Molecular Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.|Department of Anatomical Sciences Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
authors.forename: L
authors.initials: L
authors.lastname: Khorsandi
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D015870:Gene Expression; D020287:In Situ Nick-End Labeling; D008297:Male; D053768:Metal Nanoparticles; D051379:Mice; D018625:Microscopy, Atomic Force; D013737:Testis / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D014025:Titanium / Q000737:chemistry*; D019207:beta Carotene / Q000494:pharmacology*
chemical_list: D019207:beta Carotene; C009495:titanium dioxide; D014025:Titanium
keywords: Apoptosis; nanomaterials; spermatogenesis