title: Bioactive organic-inorganic poly(CLMA-co-HEA)/silica nanocomposites.
abstract: A series of novel poly(CLMA-co-HEA)/silica nanocomposites is synthesized from caprolactone 2-(methacryloyloxy)ethyl ester (CLMA) and 2-hydroxyethyl acrylate (HEA) as organic comonomers and the simultaneous sol-gel polymerization of tetraethyloxysilane (TEOS) as silica precursor, in different mass ratios up to a 30 wt% of silica. The nanocomposites are characterized as to their mechanical and thermal properties, water sorption, bioactivity and biocompatibility, reflecting the effect on the organic matrix provided by the silica network formation. The nanocomposites nucleate the growth of hydroxyapatite (HAp) on their surfaces when immersed in the simulated body fluid of the composition used in this work. Proliferation of the MC3T3 osteoblast-like cells on the materials was assessed with the MTS assay showing their biocompatibility. Immunocytochemistry reveals osteocalcin and type I collagen production, indicating that osteoblast differentiation was promoted by the materials, and calcium deposition was confirmed by von Kossa staining. The results indicate that these poly(CLMA-co-HEA)/silica nanocomposites could be a promising biomaterial for bone tissue engineering.
authors.affiliation: Center for Biomaterials and Tissue Engineering, Universitat Politècnica de València, Valencia, Spain seriv@posgrado.upv.es.
authors.forename: Sergiy
authors.initials: S
authors.lastname: Ivashchenko
authors.affiliation: Center for Biomaterials and Tissue Engineering, Universitat Politècnica de València, Valencia, Spain Present address: Institute of Biomaterial Science and Berlin-Brandenburg Center for Regenerative Therapies, Helmholtz-Zentrum Geesthacht, Teltow, Germany.
authors.forename: Jorge L
authors.initials: JL
authors.lastname: Escobar Ivirico
authors.affiliation: Center for Biomaterials and Tissue Engineering, Universitat Politècnica de València, Valencia, Spain Present address: Institute of Biomaterial Science and Berlin-Brandenburg Center for Regenerative Therapies, Helmholtz-Zentrum Geesthacht, Teltow, Germany.
authors.forename: Dunia M
authors.initials: DM
authors.lastname: García Cruz
authors.affiliation: Applied Thermodynamics Department, Universitat Politècnica de València, Valencia, Spain.
authors.forename: Alberto
authors.initials: A
authors.lastname: Campillo-Fernández
authors.affiliation: Center for Biomaterials and Tissue Engineering, Universitat Politècnica de València, Valencia, Spain Networking Research Center on Bioengineering, Biomaterials and Nanomedicine, (CIBER-BBN), Valencia, Spain.
authors.forename: Gloria
authors.initials: G
authors.lastname: Gallego Ferrer
authors.affiliation: Center for Biomaterials and Tissue Engineering, Universitat Politècnica de València, Valencia, Spain Networking Research Center on Bioengineering, Biomaterials and Nanomedicine, (CIBER-BBN), Valencia, Spain.
authors.forename: Manuel
authors.initials: M
authors.lastname: Monleón Pradas
mesh_terms: D016475:3T3 Cells; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D002118:Calcium / Q000378:metabolism; D002454:Cell Differentiation; D049109:Cell Proliferation; D024042:Collagen Type I / Q000378:metabolism; D008422:Materials Testing; D051379:Mice; D008855:Microscopy, Electron, Scanning; D053761:Nanocomposites / Q000737:chemistry* / Q000648:ultrastructure; D010006:Osteoblasts / Q000166:cytology / Q000378:metabolism; D015675:Osteocalcin / Q000378:metabolism; D011091:Polyesters / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry; D012822:Silicon Dioxide / Q000737:chemistry*; D023822:Tissue Engineering / Q000379:methods
chemical_list: D001672:Biocompatible Materials; D024042:Collagen Type I; D011091:Polyesters; D015675:Osteocalcin; C016240:polycaprolactone; D011092:Polyethylene Glycols; D012822:Silicon Dioxide; D002118:Calcium
keywords: Nanocomposites; bioactivity; biocompatibility; organic polymer; silica