title: NK1.1+ CD8+ T cells escape TGF-β control and contribute to early microbial pathogen response.
abstract: Following microbial pathogen invasion, one of the main challenges for the host is to rapidly control pathogen spreading to avoid vital tissue damage. Here we report that an effector CD8(+) T-cell population that expresses the marker NK1.1 undergoes delayed contraction and sustains early anti-microbial protection. NK1.1(+) CD8(+) T cells are derived from CD8(+) T cells during priming, and their differentiation is inhibited by transforming growth factor-β signalling. After their own contraction phase, they form a distinct pool of KLRG1 CD127 double-positive memory T cells and rapidly produce both interferon-γ and granzyme B, providing significant pathogen protection in an antigen-independent manner within only a few hours. Thus, by prolonging the CD8(+) T-cell response at the effector stage and by expressing exacerbated innate-like features at the memory stage, NK1.1(+) cells represent a distinct subset of CD8(+) T cell that contributes to the early control of microbial pathogen re-infections.
authors.affiliation: 1] Cancer Research Center of Lyon UMR INSERM 1052 CNRS 5286, Immunology, Virology and Inflammation Department, 28 rue Laennec, F-69373 08 Lyon, France [2] LabEx DEVweCAN, F-69008 Lyon, France [3] Université Lyon 1, Lyon F-69008, France [4] Centre Léon Bérard, Lyon, F-69008 Lyon, France.
authors.forename: Anne L
authors.initials: AL
authors.lastname: Ruiz
authors.affiliation: Albert Einstein College of Medicine of Yeshiva University, Microbiology and Immunology Department, Bronx, New York 10461, USA.
authors.forename: Saidi M'Homa
authors.initials: SM
authors.lastname: Soudja
authors.affiliation: 1] Cancer Research Center of Lyon UMR INSERM 1052 CNRS 5286, Immunology, Virology and Inflammation Department, 28 rue Laennec, F-69373 08 Lyon, France [2] LabEx DEVweCAN, F-69008 Lyon, France [3] Université Lyon 1, Lyon F-69008, France [4] Centre Léon Bérard, Lyon, F-69008 Lyon, France.
authors.forename: Cyril
authors.initials: C
authors.lastname: Deceneux
authors.affiliation: Albert Einstein College of Medicine of Yeshiva University, Microbiology and Immunology Department, Bronx, New York 10461, USA.
authors.forename: Grégoire
authors.initials: G
authors.lastname: Lauvau
authors.affiliation: 1] Cancer Research Center of Lyon UMR INSERM 1052 CNRS 5286, Immunology, Virology and Inflammation Department, 28 rue Laennec, F-69373 08 Lyon, France [2] LabEx DEVweCAN, F-69008 Lyon, France [3] Université Lyon 1, Lyon F-69008, France [4] Centre Léon Bérard, Lyon, F-69008 Lyon, France [5] TGF-β and Immuno-Evasion Group, Immunology and Tumor Department, DKFZ German Cancer Research Center, Heidelberg 69121, Germany.
authors.forename: Julien C
authors.initials: JC
authors.lastname: Marie
mesh_terms: D000818:Animals; D000950:Antigens, Ly / Q000378:metabolism*; D001424:Bacterial Infections / Q000276:immunology*; D001854:Bone Marrow Cells / Q000166:cytology; D018414:CD8-Positive T-Lymphocytes / Q000166:cytology*; D002454:Cell Differentiation; D005260:Female; D005434:Flow Cytometry; D053804:Granzymes / Q000378:metabolism; D007113:Immunity, Innate; D007156:Immunologic Memory; D007371:Interferon-gamma / Q000378:metabolism; D019409:Interleukin-15 / Q000378:metabolism; D053699:Interleukin-7 Receptor alpha Subunit / Q000378:metabolism; D008089:Listeria monocytogenes; D008217:Lymphocytic choriomeningitis virus; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D055651:NK Cell Lectin-Like Receptor Subfamily B / Q000378:metabolism*; D010455:Peptides / Q000737:chemistry; D010641:Phenotype; D015398:Signal Transduction; D016212:Transforming Growth Factor beta / Q000378:metabolism*; D014777:Virus Diseases / Q000276:immunology*
chemical_list: D000950:Antigens, Ly; D019409:Interleukin-15; D053699:Interleukin-7 Receptor alpha Subunit; C527211:Klrb1c protein, mouse; D055651:NK Cell Lectin-Like Receptor Subfamily B; D010455:Peptides; D016212:Transforming Growth Factor beta; D007371:Interferon-gamma; D053804:Granzymes