title: Quantitation of kudinoside A, kudinoside D and kudinoside F in human plasma using a high performance liquid chromatography-electrospray ionization tandem mass spectrometric method.
abstract: A sensitive and selective high performance liquid chromatography-tandem mass spectrometric (HPLC-MS/MS) method for the simultaneous determination of kudinoside A, kudinoside D and kudinoside F in human plasma has been firstly developed. Samples were prepared after protein precipitation and analyzed on a C18 column interfaced with a triple quadrupole tandem mass spectrometer. Negative electrospray ionization was employed as the ionization source. The mobile phase consisted of acetonitrile-water (35:65) at the flow rate of 0.3mL/min. The analytes and internal standard Ginsenoside Rb1 were both detected by use of multiple reaction monitoring mode. The method was linear in the concentration range of 2.5-1000.0ng/mL. The lower limit of quantification (LLOQ) was 2.5ng/mL. The intra-and inter-day relative standard deviation across three validation runs over the entire concentration range was less than 12.4%. The accuracy determined at three concentrations was within ±4.9% in terms of relative error. The total run time was 7.0min. This assay offers advantages in terms of expediency, and suitability for the analysis of kudinoside A, kudinoside D and kudinoside F in various biological fluids.
authors.affiliation: State Key Laboratory of Quality Research in Chinese Medicine, Macau Institute for Applied Research in Medicine and Health, Macau University of Science and Technology, Taipa, Macau, China. Electronic address: Wzhang@must.edu.mo.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Quality Research in Chinese Medicine, Macau Institute for Applied Research in Medicine and Health, Macau University of Science and Technology, Taipa, Macau, China.
authors.forename: Jianru
authors.initials: J
authors.lastname: Guo
authors.affiliation: Guangxi Key Laboratory of Traditional Chinese Medicine Quality standards (Guangxi Institute of Traditional Medical and Pharmaceutical Sciences), Nanning 530022, China.
authors.forename: Hongcong
authors.initials: H
authors.lastname: Qiu
authors.affiliation: State Key Laboratory of Quality Research in Chinese Medicine, Macau Institute for Applied Research in Medicine and Health, Macau University of Science and Technology, Taipa, Macau, China.
authors.forename: Caiyun
authors.initials: C
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Quality Research in Chinese Medicine, Macau Institute for Applied Research in Medicine and Health, Macau University of Science and Technology, Taipa, Macau, China.
authors.forename: Qian Qian
authors.initials: QQ
authors.lastname: Chen
authors.affiliation: Guangxi Key Laboratory of Traditional Chinese Medicine Quality standards (Guangxi Institute of Traditional Medical and Pharmaceutical Sciences), Nanning 530022, China.
authors.forename: Buming
authors.initials: B
authors.lastname: Liu
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D057230:Limit of Detection; D015203:Reproducibility of Results; D012503:Saponins / Q000097:blood*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods*; D014315:Triterpenes / Q000097:blood*
chemical_list: D012503:Saponins; D014315:Triterpenes; C102126:kudinoside A; C580614:kudinoside D; C580612:kudinoside F
keywords: HPLC–MS/MS; Human plasma; Kudinoside A; Kudinoside D; Kudinoside F