title: Effects of various organic carbon sources on the growth and biochemical composition of Chlorella pyrenoidosa.
abstract: The aim of this study was to investigate the effects of various organic carbon sources (glucose, galactose, fructose, sucrose, maltose, lactose and starch) on the growth and biochemical composition of Chlorella pyrenoidosa. Monosaccharides were found to exert stronger stimulative effects on the algal growth than disaccharides and starch. After 10-day culture, addition of 0.5-5.0 g L(-1) glucose and galactose significantly reduced the cellular protein contents by 27.7-63.7% and 22.6-60.5%, respectively, and significantly increased the carbohydrate contents by 103.2-266.5% and 91.9-240.0%, respectively. However, addition of 0.5-5.0 g L(-1) disaccharides and starch did not significantly affect the contents of lipid, protein and carbohydrate. Similar to the normal nitrogen condition, the cellular biochemical composition was not significantly affected by addition of 3.0 g L(-1) disaccharides and starch under the low nitrogen condition. Finally, the significance of this work in the biotechnological application of mixotrophic cultivation of C. pyrenoidosa was further discussed.
authors.affiliation: State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing 210093, China.
authors.forename: Weiguo
authors.initials: W
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing 210093, China.
authors.forename: Peiliang
authors.initials: P
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing 210093, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Sun
authors.affiliation: State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing 210093, China.
authors.forename: Maozhen
authors.initials: M
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing 210093, China.
authors.forename: Shan
authors.initials: S
authors.lastname: Lu
authors.affiliation: State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing 210093, China. Electronic address: pengfuli@nju.edu.cn.
authors.forename: Pengfu
authors.initials: P
authors.lastname: Li
mesh_terms: D061249:Batch Cell Culture Techniques / Q000379:methods; D049109:Cell Proliferation / Q000187:drug effects / Q000502:physiology*; D002478:Cells, Cultured; D002708:Chlorella / Q000166:cytology / Q000502:physiology*; D004187:Disaccharides / Q000378:metabolism* / Q000494:pharmacology; D009005:Monosaccharides / Q000378:metabolism* / Q000494:pharmacology*; D009584:Nitrogen / Q000378:metabolism; D010940:Plant Proteins / Q000378:metabolism*; D013213:Starch / Q000378:metabolism* / Q000494:pharmacology
chemical_list: D004187:Disaccharides; D009005:Monosaccharides; D010940:Plant Proteins; D013213:Starch; D009584:Nitrogen
keywords: Biochemical composition; Microalgae; Mixotrophic growth; Starch; Sugar