title: Association of endothelial nitric oxide synthase gene G894T polymorphism and serum nitric oxide levels in patients with preeclampsia and gestational hypertension.
abstract: OBJECTIVE
  Pregnancy-induced hypertension is one of the most important cause of maternal-fetal morbidity and mortality. Pregnancy-related hypertensive disorders are usually associated with diminished nitric oxide (NO) levels. We aimed to evaluate the role of serum NO levels and eNOS gene G894T polymorphism on hypertensive disorders of pregnancy.
  
  
  METHODS
  Eighty patients with gestational hypertension or preeclampsia, and 80 healthy pregnants were enrolled to analyze serum NO levels and G894T polymorphism of the eNOS gene. NO level was analyzed by high-performance liquid chromatography (HPLC) method. The G894T polymorphism of the eNOS gene was determined by polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP).
  
  
  RESULTS
  There was no significant difference between groups in terms of G894T/eNOS genotype and allele frequencies (p > 0.05). Serum NO levels were significantly lower in the patients group. In the control group, subjects with thymine-thymine (TT) genotype had significantly lower NO levels when compared to subjects with guanine-guanine (GG) or guanine-thymine (GT) genotype (p < 0.05).
  
  
  CONCLUSIONS
  We failed to demonstrate an association between eNOS gene G894T polymorphism and serum NO levels in patients with pregnancy-induced hypertensive disorders. We established a relation between pregnancy-induced hypertension and low NO levels.
authors.affiliation: a Department of Obstetrics and Gynecology , Suleymaniye Maternity Education and Research Hospital , Istanbul , Turkey .
authors.forename: Mehmet Nafi
authors.initials: MN
authors.lastname: Sakar
authors.affiliation: b Department of Internal Medicine , Bagcilar Education and Research Hospital , Istanbul , Turkey .
authors.forename: Ahmet Engin
authors.initials: AE
authors.lastname: Atay
authors.affiliation: c Department of Obstetrics and Gynecology , Haseki Education and Research Hospital , Istanbul , Turkey .
authors.forename: Sureyya
authors.initials: S
authors.lastname: Demir
authors.affiliation: c Department of Obstetrics and Gynecology , Haseki Education and Research Hospital , Istanbul , Turkey .
authors.forename: Vuslat Lale
authors.initials: VL
authors.lastname: Bakir
authors.affiliation: c Department of Obstetrics and Gynecology , Haseki Education and Research Hospital , Istanbul , Turkey .
authors.forename: Bulent
authors.initials: B
authors.lastname: Demir
authors.affiliation: d Department of Obstetrics and Gynecology , MH Diyarbakir Obstetrics and Gynecology Hospital , Diyarbakir , Turkey , and.
authors.forename: Deniz
authors.initials: D
authors.lastname: Balsak
authors.affiliation: a Department of Obstetrics and Gynecology , Suleymaniye Maternity Education and Research Hospital , Istanbul , Turkey .
authors.forename: Emrullah
authors.initials: E
authors.lastname: Akay
authors.affiliation: e Department of General Surgery , Suleymaniye Maternity Education and Research Hospital , Istanbul , Turkey.
authors.forename: A Ibrahim
authors.initials: AI
authors.lastname: Ulusoy
authors.affiliation: a Department of Obstetrics and Gynecology , Suleymaniye Maternity Education and Research Hospital , Istanbul , Turkey .
authors.forename: Fatma Ferda
authors.initials: FF
authors.lastname: Verit
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D002851:Chromatography, High Pressure Liquid; D005260:Female; D005787:Gene Frequency; D005819:Genetic Markers; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D046110:Hypertension, Pregnancy-Induced / Q000097:blood / Q000235:genetics*; D009569:Nitric Oxide / Q000097:blood*; D052250:Nitric Oxide Synthase Type III / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D011225:Pre-Eclampsia / Q000097:blood / Q000235:genetics; D011247:Pregnancy; D014421:Turkey
chemical_list: D005819:Genetic Markers; D009569:Nitric Oxide; C496314:NOS3 protein, human; D052250:Nitric Oxide Synthase Type III
keywords: Gene polymorphism; gestational hypertension; nitric oxide; nitric oxide synthase; preeclampsia