title: Structural modulation of gut microbiota during alleviation of type 2 diabetes with a Chinese herbal formula.
abstract: The gut microbiota is hypothesized to have a critical role in metabolic diseases, including type 2 diabetes (T2D). A traditional Chinese herbal formula, Gegen Qinlian Decoction (GQD), can alleviate T2D. To find out whether GQD modulates the composition of the gut microbiota during T2D treatment, 187 T2D patients were randomly allocated to receive high (HD, n=44), moderate (MD, n=52), low dose GQD (LD, n=50) or the placebo (n=41) for 12 weeks in a double-blinded trial. Patients who received the HD or MD demonstrated significant reductions in adjusted mean changes from baseline of fasting blood glucose (FBG) and glycated hemoglobin (HbA1c) compared with the placebo and LD groups. Pyrosequencing of the V3 regions of 16S rRNA genes revealed a dose-dependent deviation of gut microbiota in response to GQD treatment. This deviation occurred before significant improvement of T2D symptoms was observed. Redundancy analysis identified 47 GQD-enriched species level phylotypes, 17 of which were negatively correlated with FBG and 9 with HbA1c. Real-time quantitative PCR confirmed that GQD significantly enriched Faecalibacterium prausnitzii, which was negatively correlated with FBG, HbA1c and 2-h postprandial blood glucose levels and positively correlated with homeostasis model assessment of β-cell function. Therefore, these data indicate that structural changes of gut microbiota are induced by Chinese herbal formula GQD. Specifically, GQD treatment may enrich the amounts of beneficial bacteria, such as Faecalibacterium spp. In conclusion, changes in the gut microbiota are associated with the anti-diabetic effects of GQD.
authors.affiliation: State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, People's Republic of China.
authors.forename: Jia
authors.initials: J
authors.lastname: Xu
authors.affiliation: Guang'anmen Hospital, China Academy of Chinese Medical Sciences, Beijing, People's Republic of China.
authors.forename: Fengmei
authors.initials: F
authors.lastname: Lian
authors.affiliation: Guang'anmen Hospital, China Academy of Chinese Medical Sciences, Beijing, People's Republic of China.
authors.forename: Linhua
authors.initials: L
authors.lastname: Zhao
authors.affiliation: Ministry of Education Key Laboratory of Systems Biomedicine, Shanghai Center for Systems Biomedicine, Shanghai Jiao Tong University, Shanghai, People's Republic of China.
authors.forename: Yufeng
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Guang'anmen Hospital, China Academy of Chinese Medical Sciences, Beijing, People's Republic of China.
authors.forename: Xinyan
authors.initials: X
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, People's Republic of China.
authors.forename: Xu
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Guang'anmen Hospital, China Academy of Chinese Medical Sciences, Beijing, People's Republic of China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Guo
authors.affiliation: State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, People's Republic of China.
authors.forename: Chenhong
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Guang'anmen Hospital, China Academy of Chinese Medical Sciences, Beijing, People's Republic of China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Zhou
authors.affiliation: State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, People's Republic of China.
authors.forename: Zhengsheng
authors.initials: Z
authors.lastname: Xue
authors.affiliation: State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, People's Republic of China.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Pang
authors.affiliation: 1] State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, People's Republic of China [2] Ministry of Education Key Laboratory of Systems Biomedicine, Shanghai Center for Systems Biomedicine, Shanghai Jiao Tong University, Shanghai, People's Republic of China.
authors.forename: Liping
authors.initials: L
authors.lastname: Zhao
authors.affiliation: Guang'anmen Hospital, China Academy of Chinese Medical Sciences, Beijing, People's Republic of China.
authors.forename: Xiaolin
authors.initials: X
authors.lastname: Tong
mesh_terms: D000328:Adult; D000368:Aged; D001419:Bacteria / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification; D001786:Blood Glucose / Q000378:metabolism; D003924:Diabetes Mellitus, Type 2 / Q000188:drug therapy* / Q000378:metabolism / Q000382:microbiology*; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage*; D005260:Female; D041981:Gastrointestinal Tract / Q000187:drug effects / Q000382:microbiology*; D006442:Glycated Hemoglobin / Q000378:metabolism; D006801:Humans; D008297:Male; D064307:Microbiota / Q000187:drug effects*; D008875:Middle Aged
chemical_list: D001786:Blood Glucose; D004365:Drugs, Chinese Herbal; D006442:Glycated Hemoglobin