title: Optimization of a DNA nicking assay to evaluate Oenocarpus bataua and Camellia sinensis antioxidant capacity.
abstract: This study was aimed at assessing the DNA damage protective activity of different types of extracts (aqueous, methanolic and acetonic) using an in vitro DNA nicking assay. Several parameters were optimized using the pUC18 plasmid, especially FeSO4, EDTA, solvent concentrations and incubation time. Special attention has been paid to removing the protective and damaging effect of the solvent and FeSO4 respectively, as well as to identifying the relevant positive and negative controls. For each solvent, the optimal conditions were determined: (i) for aqueous extracts, 0.33 mM of FeSO4 and 0.62 mM of EDTA were incubated for 20 min at 37 °C; (ii) for acetone extracts, 1.16% solvent were incubated for 15 min at 37 °C with 1.3 mM of FeSO4 and 2.5 mM of EDTA and (iii) for methanol extracts, 0.16% solvent, were incubated for 1.5 h at 37 °C with 0.33 mM of FeSO4 and 0.62 mM of EDTA. Using the optimized conditions, the DNA damage protective activity of aqueous, methanolic and acetonic extracts of an Amazonian palm berry (Oenocarpus bataua) and green tea (Camellia sinensis) was assessed. Aqueous and acetonic Oenocarpus bataua extracts were protective against DNA damage, whereas aqueous, methanolic and acetonic extracts of Camellia sinensis extracts induced DNA damage.
authors.affiliation: Université des Antilles et de la Guyane, UMR QUALITROP, campus universitaire de Troubiran, P.O. Box 792, 97337 Cayenne Cedex, French Guiana, France. ljleba@pasteur-cayenne.fr.
authors.forename: Louis-Jérôme
authors.initials: LJ
authors.lastname: Leba
authors.affiliation: Université des Antilles et de la Guyane, UMR QUALITROP, campus universitaire de Troubiran, P.O. Box 792, 97337 Cayenne Cedex, French Guiana, France. christel.brunschwig@gmail.com.
authors.forename: Christel
authors.initials: C
authors.lastname: Brunschwig
authors.affiliation: Université des Antilles et de la Guyane, UMR QUALITROP, campus universitaire de Troubiran, P.O. Box 792, 97337 Cayenne Cedex, French Guiana, France. mona_saout@hotmail.com.
authors.forename: Mona
authors.initials: M
authors.lastname: Saout
authors.affiliation: Université des Antilles et de la Guyane, UMR QUALITROP, campus universitaire de Troubiran, P.O. Box 792, 97337 Cayenne Cedex, French Guiana, France. karine.martial@guyane.univ-ag.fr.
authors.forename: Karine
authors.initials: K
authors.lastname: Martial
authors.affiliation: Université des Antilles et de la Guyane, UMR QUALITROP, campus universitaire de Troubiran, P.O. Box 792, 97337 Cayenne Cedex, French Guiana, France. ema_vulcain@yahoo.fr.
authors.forename: Emmanuelle
authors.initials: E
authors.lastname: Vulcain
authors.affiliation: Université des Antilles et de la Guyane, UMR QUALITROP, campus universitaire de Troubiran, P.O. Box 792, 97337 Cayenne Cedex, French Guiana, France. didier.bereau@guyane.univ-ag.fr.
authors.forename: Didier
authors.initials: D
authors.lastname: Bereau
authors.affiliation: Université des Antilles et de la Guyane, UMR QUALITROP, campus universitaire de Troubiran, P.O. Box 792, 97337 Cayenne Cedex, French Guiana, France. jean-charles.robinson@guyane.univ-ag.fr.
authors.forename: Jean-Charles
authors.initials: JC
authors.lastname: Robinson
mesh_terms: D000975:Antioxidants / Q000737:chemistry* / Q000378:metabolism; D028023:Arecaceae / Q000737:chemistry* / Q000378:metabolism; D028241:Camellia sinensis / Q000737:chemistry* / Q000378:metabolism; D002839:Chromans / Q000737:chemistry / Q000378:metabolism; D053904:DNA Breaks, Single-Stranded; D003850:Deoxyribonuclease I / Q000378:metabolism*; D057075:Enzyme Assays; D005707:Gallic Acid / Q000737:chemistry / Q000378:metabolism; D017665:Hydroxyl Radical / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry* / Q000378:metabolism; D018515:Plant Leaves / Q000737:chemistry / Q000378:metabolism; D010957:Plasmids / Q000235:genetics / Q000378:metabolism; D011794:Quercetin / Q000737:chemistry / Q000378:metabolism
chemical_list: D000975:Antioxidants; D002839:Chromans; D010936:Plant Extracts; D017665:Hydroxyl Radical; D005707:Gallic Acid; D011794:Quercetin; D003850:Deoxyribonuclease I; C010643:6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid