title: Single nucleotide polymorphism analysis of Korean native chickens using next generation sequencing data.
abstract: There are five native chicken lines in Korea, which are mainly classified by plumage colors (black, white, red, yellow, gray). These five lines are very important genetic resources in the Korean poultry industry. Based on a next generation sequencing technology, whole genome sequence and reference assemblies were performed using Gallus_gallus_4.0 (NCBI) with whole genome sequences from these lines to identify common and novel single nucleotide polymorphisms (SNPs). We obtained 36,660,731,136 ± 1,257,159,120 bp of raw sequence and average 26.6-fold of 25-29 billion reference assembly sequences representing 97.288 % coverage. Also, 4,006,068 ± 97,534 SNPs were observed from 29 autosomes and the Z chromosome and, of these, 752,309 SNPs are the common SNPs across lines. Among the identified SNPs, the number of novel- and known-location assigned SNPs was 1,047,951 ± 14,956 and 2,948,648 ± 81,414, respectively. The number of unassigned known SNPs was 1,181 ± 150 and unassigned novel SNPs was 8,238 ± 1,019. Synonymous SNPs, non-synonymous SNPs, and SNPs having character changes were 26,266 ± 1,456, 11,467 ± 604, 8,180 ± 458, respectively. Overall, 443,048 ± 26,389 SNPs in each bird were identified by comparing with dbSNP in NCBI. The presently obtained genome sequence and SNP information in Korean native chickens have wide applications for further genome studies such as genetic diversity studies to detect causative mutations for economic and disease related traits.
authors.affiliation: Department of Animal Science and Biotechnology, Chungnam National University, Daejeon, 305-764, Korea.
authors.forename: Dong-Won
authors.initials: DW
authors.lastname: Seo
authors.forename: Jae-Don
authors.initials: JD
authors.lastname: Oh
authors.forename: Shil
authors.initials: S
authors.lastname: Jin
authors.forename: Ki-Duk
authors.initials: KD
authors.lastname: Song
authors.forename: Hee-Bok
authors.initials: HB
authors.lastname: Park
authors.forename: Kang-Nyeong
authors.initials: KN
authors.lastname: Heo
authors.forename: Younhee
authors.initials: Y
authors.lastname: Shin
authors.forename: Myunghee
authors.initials: M
authors.lastname: Jung
authors.forename: Junhyung
authors.initials: J
authors.lastname: Park
authors.forename: Cheorun
authors.initials: C
authors.lastname: Jo
authors.forename: Hak-Kyo
authors.initials: HK
authors.lastname: Lee
authors.forename: Jun-Heon
authors.initials: JH
authors.lastname: Lee
mesh_terms: D000818:Animals; D002645:Chickens / Q000235:genetics*; D023281:Genomics; D059014:High-Throughput Nucleotide Sequencing; D054643:INDEL Mutation; D008297:Male; D020641:Polymorphism, Single Nucleotide; D056910:Republic of Korea