title: si-RNA-Mediated Silencing of ADRBK1 Gene Attenuates Breast Cancer Cell Proliferation.
abstract: Abstract Breast cancer is the most prominent cause of cancer-related deaths among women worldwide. It has been found that genetic mutations play distinct roles in the onset and progression of breast cancer. Androgenic, beta, receptor kinase 1 (ADRBK1) has been reported to possess oncogenic characteristics vital for cancer cell viability. This study was designed to investigate the effects of small interference RNA (si-RNA)-mediated ADRBK1 knockdown on breast cancer cell growth in vitro. High-expression levels of ADRBK1 were observed in all tested breast cancer cell lines (MDA-MB-231, MCF-7, T-47D, and BT-474). ADRBK1 si-RNA was delivered to breast cancer cells using lentivirus delivery system. Depletion of ADRBK1 significantly attenuated the cell viability and colony-formation ability. Flow cytometry analysis further demonstrated that ADRBK1 silencing led to MDA-MB-231 cell arrest in the G0/G1 phase. Collectively, these results indicate that knockdown of ADRBK1 gene has detrimental effects on breast cancer cell growth, which may be a potential therapeutic approach for the treatment of breast cancer.
authors.affiliation: 1 Shanghai Tenth People's Hospital, Tongji University School of Medicine , Shanghai, China .
authors.forename: Chen
authors.initials: C
authors.lastname: Zhang
authors.forename: Xianzhen
authors.initials: X
authors.lastname: Chen
authors.forename: Yongxin
authors.initials: Y
authors.lastname: Li
authors.forename: Himaya
authors.initials: H
authors.lastname: S W A
authors.forename: Jie
authors.initials: J
authors.lastname: Wu
authors.forename: Xiujuan
authors.initials: X
authors.lastname: Shi
authors.forename: Xiaoqing
authors.initials: X
authors.lastname: Liu
authors.forename: Sekwon
authors.initials: S
authors.lastname: Kim
mesh_terms: D017209:Apoptosis / Q000235:genetics; D001943:Breast Neoplasms / Q000201:enzymology / Q000235:genetics / Q000628:therapy*; D048708:Cell Growth Processes / Q000235:genetics; D045744:Cell Line, Tumor; D005260:Female; D054769:G-Protein-Coupled Receptor Kinase 2 / Q000172:deficiency / Q000235:genetics* / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic; D055785:Gene Knockdown Techniques; D020868:Gene Silencing; D006801:Humans; D061986:MCF-7 Cells; D034741:RNA, Small Interfering / Q000008:administration & dosage* / Q000235:genetics*; D014162:Transfection
chemical_list: D034741:RNA, Small Interfering; C496567:GRK2 protein, human; D054769:G-Protein-Coupled Receptor Kinase 2
keywords: ADRBK1; breast cancer; cell growth; si-RNA