title: Carbon nanoparticle from a natural source fabricated for folate receptor targeting, imaging and drug delivery application in A549 lung cancer cells.
abstract: There is an emerging need for the development of new anticancer nanocomposite which exhibits imaging properties and targeted drug delivery. In the present study, a nanobiocomposite was prepared, in this direction, which contains carbon nanoparticles (CNP), methotrexate (Mtx) and asparaginase (Asp) coupled with fluorescein isothiocyanate (FITC). The prepared nanobiocomposite kills only the cancer cells due to the presence of Mtx which is a folic acid analogue and targets the cancer cells due to the over expression of folate receptors on their surface and apoptosis occurs due to the anticancer activity of enzyme asparaginase. The results obtained from the present study confirmed the sustained release of Mtx and Asp from the nanobiocomposite. The nanobiocomposite was found to be haemocompatible, biocompatible and showed more than 90% apoptosis. The drug pathway was clearly monitored due to the presence of FITC in the nanobiocomposite. This study proves the effectiveness of the fabricated nanoconjugate, as it helps in imaging, targeting and destructing the cancerous cells. The prepared nanoconjugate may be effectively applied in in vivo experiments before applying on to humans.
authors.affiliation: Bio-Products Laboratory, Central Leather Research Institute, Chennai, India.
authors.forename: Thangavelu
authors.initials: T
authors.lastname: Muthukumar
authors.affiliation: St. Joseph's College of Engineering, Chennai, India.
authors.forename: Munusamy
authors.initials: M
authors.lastname: Chamundeeswari
authors.affiliation: Department of Biotechnology, SRM University, Chennai, India.
authors.forename: Sundaram
authors.initials: S
authors.lastname: Prabhavathi
authors.affiliation: St. Joseph's College of Engineering, Chennai, India.
authors.forename: Baskar
authors.initials: B
authors.lastname: Gurunathan
authors.affiliation: St. Joseph's College of Engineering, Chennai, India.
authors.forename: J
authors.initials: J
authors.lastname: Chandhuru
authors.affiliation: Bio-Products Laboratory, Central Leather Research Institute, Chennai, India. Electronic address: sastrytp@hotmail.com.
authors.forename: Thotapalli Parvathaleswara
authors.initials: TP
authors.lastname: Sastry
mesh_terms: D016475:3T3 Cells; D000818:Animals; D000970:Antineoplastic Agents / Q000008:administration & dosage* / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects; D001215:Asparaginase / Q000008:administration & dosage* / Q000494:pharmacology; D002244:Carbon / Q000737:chemistry*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D004337:Drug Carriers / Q000737:chemistry*; D065546:Drug Liberation; D058972:Folic Acid Transporters / Q000378:metabolism*; D006461:Hemolysis / Q000187:drug effects; D006801:Humans; D008727:Methotrexate / Q000008:administration & dosage* / Q000494:pharmacology; D051379:Mice; D018625:Microscopy, Atomic Force; D053761:Nanocomposites / Q000008:administration & dosage / Q000737:chemistry; D053758:Nanoparticles / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D013499:Surface Properties / Q000187:drug effects
chemical_list: D000970:Antineoplastic Agents; D004337:Drug Carriers; D058972:Folic Acid Transporters; D002244:Carbon; D001215:Asparaginase; D008727:Methotrexate
keywords: Asparaginase; Cancer; Carbon nanoparticle; Drug delivery; Fluorescein isothiocyanate; Methotrexate