title: Feasibility Study for Detection of Turnip yellow mosaic virus (TYMV) Infection of Chinese Cabbage Plants Using Raman Spectroscopy.
abstract: Raman spectroscopy provides many advantages compared to other common analytical techniques due to its ability of rapid and accurate identification of unknown specimens as well as simple sample preparation. Here, we described potential of Raman spectroscopic technique as an efficient and high throughput method to detect plants infected by economically important viruses. To enhance the detection sensitivity of Raman measurement, surface enhanced Raman scattering (SERS) was employed. Spectra of extracts from healthy and Turnip yellow mosaic virus (TYMV) infected Chinese cabbage leaves were collected by mixing with gold (Au) nanoparticles. Our result showed that TYMV infected plants could be discriminated from non-infected healthy plants, suggesting the current method described here would be an alternative potential tool to screen virus-infection of plants in fields although it needs more studies to generalize the technique.
authors.affiliation: Department of Chemistry, Hanyang University, Seoul 133-791, Korea.
authors.forename: Saetbyeol
authors.initials: S
authors.lastname: Kim
authors.affiliation: Department of Chemistry, Hanyang University, Seoul 133-791, Korea.
authors.forename: Sanguk
authors.initials: S
authors.lastname: Lee
authors.affiliation: Smarteome. Co., Ltd., Suwon 441-853, Korea.
authors.forename: Hee-Youn
authors.initials: HY
authors.lastname: Chi
authors.affiliation: Agricultural Microbiology Division, National Academy of Agricultural Science, RDA, Suwon 441-707, Korea.
authors.forename: Mi-Kyeong
authors.initials: MK
authors.lastname: Kim
authors.affiliation: Agricultural Microbiology Division, National Academy of Agricultural Science, RDA, Suwon 441-707, Korea.
authors.forename: Jeong-Soo
authors.initials: JS
authors.lastname: Kim
authors.affiliation: School of Applied Biosciences, College of Agriculture & Life Scieneces, Kyungpook National University, Daegu 702-701, Korea.
authors.forename: Su-Heon
authors.initials: SH
authors.lastname: Lee
authors.affiliation: Department of Chemistry, Hanyang University, Seoul 133-791, Korea.
authors.forename: Hoeil
authors.initials: H
authors.lastname: Chung
keywords: Raman spectroscopy; Turnip yellow mosaic virus (TYMV); discrimination; surface enhanced Raman scattering (SERS); virus detection