title: Isolation and in silico analysis of Fe-superoxide dismutase in the cyanobacterium Nostoc commune.
abstract: Cyanobacteria are known to endure various stress conditions due to the inbuilt potential for oxidative stress alleviation owing to the presence of an array of antioxidants. The present study shows that Antarctic cyanobacterium Nostoc commune possesses two antioxidative enzymes viz., superoxide dismutase (SOD) and catalase that jointly cope with environmental stresses prevailing at its natural habitat. Native-PAGE analysis illustrates the presence of a single prominent isoform recognized as Fe-SOD and three distinct isoforms of catalase. The protein sequence of Fe-SOD in N. commune retrieved from NCBI protein sequence database was used for in silico analysis. 3D structure of N. commune was predicted by comparative modeling using MODELLER 9v11. Further, this model was validated for its quality by Ramachandran plot, ERRAT, Verify 3D and ProSA-web which revealed good structure quality of the model. Multiple sequence alignment showed high conservation in N and C-terminal domain regions along with all metal binding positions in Fe-SOD which were also found to be highly conserved in all 28 cyanobacterial species under study, including N. commune. In silico prediction of isoelectric point and molecular weight of Fe-SOD was found to be 5.48 and 22,342.98Da respectively. The phylogenetic tree revealed that among 28 cyanobacterial species, Fe-SOD in N. commune was the closest evolutionary homolog of Fe-SOD in Nostoc punctiforme as evident by strong bootstrap value. Thus, N. commune may serve as a good biological model for studies related to survival of life under extreme conditions prevailing at the Antarctic region. Moreover cyanobacteria may be exploited for biochemical and biotechnological applications of enzymatic antioxidants.
authors.affiliation: Laboratory of Photobiology and Molecular Microbiology, Centre of Advanced Study in Botany, Banaras Hindu University, Varanasi 221005, India.
authors.forename: Minu
authors.initials: M
authors.lastname: Kesheri
authors.affiliation: Department of Biological Science, Birla Institute of Technology and Science, Pilani, Rajasthan 333031, India.
authors.forename: Swarna
authors.initials: S
authors.lastname: Kanchan
authors.affiliation: Laboratory of Photobiology and Molecular Microbiology, Centre of Advanced Study in Botany, Banaras Hindu University, Varanasi 221005, India.
authors.lastname: Richa
authors.affiliation: Laboratory of Photobiology and Molecular Microbiology, Centre of Advanced Study in Botany, Banaras Hindu University, Varanasi 221005, India. Electronic address: r.p.sinha@gmx.net.
authors.forename: Rajeshwar P
authors.initials: RP
authors.lastname: Sinha
mesh_terms: D000595:Amino Acid Sequence; D003198:Computer Simulation; D008958:Models, Molecular; D008969:Molecular Sequence Data; D063347:Native Polyacrylamide Gel Electrophoresis; D046937:Nostoc / Q000201:enzymology*; D010802:Phylogeny; D017386:Sequence Homology, Amino Acid; D013482:Superoxide Dismutase / Q000737:chemistry / Q000145:classification / Q000302:isolation & purification*
chemical_list: D013482:Superoxide Dismutase
keywords: Catalase; Cyanobacteria; Fe-SOD; Homology modeling; Native-PAGE; Phylogenetic analysis