title: Construction of Infectious cDNA Clone of a Chrysanthemum stunt viroid Korean Isolate.
abstract: Chrysanthemum stunt viroid (CSVd), a noncoding infectious RNA molecule, causes seriously economic losses of chrysanthemum for 3 or 4 years after its first infection. Monomeric cDNA clones of CSVd isolate SK1 (CSVd-SK1) were constructed in the plasmids pGEM-T easy vector and pUC19 vector. Linear positive-sense transcripts synthesized in vitro from the full-length monomeric cDNA clones of CSVd-SK1 could infect systemically tomato seedlings and chrysanthemum plants, suggesting that the linear CSVd RNA transcribed from the cDNA clones could be replicated as efficiently as circular CSVd in host species. However, direct inoculation of plasmid cDNA clones containing full-length monomeric cDNA of CSVd-SK1 failed to infect tomato and chrysanthemum and linear negative-sense transcripts from the plasmid DNAs were not infectious in the two plant species. The cDNA sequences of progeny viroid in systemically infected tomato and chrysanthemum showed a few substitutions at a specific nucleotide position, but there were no deletions and insertions in the sequences of the CSVd progeny from tomato and chrysanthemum plants.
authors.affiliation: Department of Horticulture and Landscape, Seoul Women's University, Seoul 139-774, Korea.
authors.forename: Ju-Yeon
authors.initials: JY
authors.lastname: Yoon
authors.affiliation: Department of Horticultural Environment, National Institute of Horticultural and Herbal Science, RDA 441-440, Korea.
authors.forename: In-Sook
authors.initials: IS
authors.lastname: Cho
authors.affiliation: Department of Horticultural Environment, National Institute of Horticultural and Herbal Science, RDA 441-440, Korea.
authors.forename: Gug-Seoun
authors.initials: GS
authors.lastname: Choi
authors.affiliation: Department of Horticultural Environment, National Institute of Horticultural and Herbal Science, RDA 441-440, Korea.
authors.forename: Seung-Kook
authors.initials: SK
authors.lastname: Choi
keywords: Chrysanthemum; Chrysanthemum stunt viroid; RT-PCR; infectious cDNA; tomato