title: Techniques for assessing the effects of pharmacological inhibitors on intercellular protein movement.
abstract: Intercellular protein movement is an important mechanism in plant development. Here we present an integrated protocol that utilizes an inducible system to block plasmodesmata-dependent movement and assessment of fluorescent recovery after photobleaching (FRAP) to identify compounds that influence intercellular protein movement.
authors.affiliation: 121 Lynch Laboratories, University of Pennsylvania, Philadelphia, PA, 19104, USA.
authors.forename: Shuang
authors.initials: S
authors.lastname: Wu
authors.forename: Kimberly L
authors.initials: KL
authors.lastname: Gallagher
mesh_terms: D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism; D015638:Cytochalasin D / Q000494:pharmacology; D004136:Dinitrobenzenes / Q000494:pharmacology; D004958:Estradiol / Q000494:pharmacology; D036681:Fluorescence Recovery After Photobleaching / Q000379:methods*; D018506:Gene Expression Regulation, Plant; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D057054:Molecular Imaging; D030821:Plants, Genetically Modified; D010957:Plasmids / Q000737:chemistry / Q000378:metabolism; D031425:Plasmodesmata / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D021381:Protein Transport; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D036226:Seedlings / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D013424:Sulfanilamides / Q000494:pharmacology; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism; D019076:Transgenes; D050257:Tubulin Modulators / Q000494:pharmacology
chemical_list: D029681:Arabidopsis Proteins; D004136:Dinitrobenzenes; D011993:Recombinant Fusion Proteins; C410510:SHORT ROOT protein, Arabidopsis; D013424:Sulfanilamides; D014157:Transcription Factors; D050257:Tubulin Modulators; D049452:Green Fluorescent Proteins; D015638:Cytochalasin D; D004958:Estradiol; C012465:oryzalin