title: Salt tolerant SUV3 overexpressing transgenic rice plants conserve physicochemical properties and microbial communities of rhizosphere.
abstract: Key concerns in the ecological evaluation of GM crops are undesirably spread, gene flow, other environmental impacts, and consequences on soil microorganism's biodiversity. Numerous reports have highlighted the effects of transgenic plants on the physiology of non-targeted rhizospheric microbes and the food chain via causing adverse effects. Therefore, there is an urgent need to develop transgenics with insignificant toxic on environmental health. In the present study, SUV3 overexpressing salt tolerant transgenic rice evaluated in New Delhi and Cuttack soil conditions for their effects on physicochemical and biological properties of rhizosphere. Its cultivation does not affect soil properties viz., pH, Eh, organic C, P, K, N, Ca, Mg, S, Na and Fe(2+). Additionally, SUV3 rice plants do not cause any change in the phenotype, species characteristics and antibiotic sensitivity of rhizospheric bacteria. The population and/or number of soil organisms such as bacteria, fungi and nematodes were unchanged in the soil. Also, the activity of bacterial enzymes viz., dehydrogenase, invertase, phenol oxidases, acid phosphatases, ureases and proteases was not significantly affected. Further, plant growth promotion (PGP) functions of bacteria such as siderophore, HCN, salicylic acid, IAA, GA, zeatin, ABA, NH3, phosphorus metabolism, ACC deaminase and iron tolerance were, considerably, not influenced. The present findings suggest ecologically pertinent of salt tolerant SUV3 rice to sustain the health and usual functions of the rhizospheric organisms.
authors.affiliation: Plant Molecular Biology Group, International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi 110 067, India.
authors.forename: Ranjan K
authors.initials: RK
authors.lastname: Sahoo
authors.affiliation: Plant Molecular Biology Group, International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi 110 067, India.
authors.forename: Mohammad W
authors.initials: MW
authors.lastname: Ansari
authors.affiliation: Plant Molecular Biology Group, International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi 110 067, India.
authors.forename: Renu
authors.initials: R
authors.lastname: Tuteja
authors.affiliation: Plant Molecular Biology Group, International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi 110 067, India. Electronic address: narendra@icgeb.res.in.
authors.forename: Narendra
authors.initials: N
authors.lastname: Tuteja
mesh_terms: D000704:Analysis of Variance; D001419:Bacteria / Q000378:metabolism; D001483:Base Sequence; D044822:Biodiversity; D053487:DEAD-box RNA Helicases / Q000235:genetics / Q000378:metabolism*; D017931:DNA Primers / Q000235:genetics; D004553:Electric Conductivity; D006863:Hydrogen-Ion Concentration; D007194:India; D008969:Molecular Sequence Data; D012275:Oryza / Q000201:enzymology / Q000502:physiology*; D030821:Plants, Genetically Modified / Q000009:adverse effects / Q000382:microbiology / Q000502:physiology*; D058441:Rhizosphere; D017422:Sequence Analysis, DNA; D012987:Soil / Q000737:chemistry; D012988:Soil Microbiology
chemical_list: D017931:DNA Primers; D012987:Soil; D053487:DEAD-box RNA Helicases
keywords: Abiotic stress; Enzyme activity; Microorganism population; Plant growth promotion; SUV3 rice transgenic; Soil ecology