title: Irreversible fate commitment in the Arabidopsis stomatal lineage requires a FAMA and RETINOBLASTOMA-RELATED module.
abstract: The presumed totipotency of plant cells leads to questions about how specific stem cell lineages and terminal fates could be established. In the Arabidopsis stomatal lineage, a transient self-renewing phase creates precursors that differentiate into one of two epidermal cell types, guard cells or pavement cells. We found that irreversible differentiation of guard cells involves RETINOBLASTOMA-RELATED (RBR) recruitment to regulatory regions of master regulators of stomatal initiation, facilitated through interaction with a terminal stomatal lineage transcription factor, FAMA. Disrupting physical interactions between FAMA and RBR preferentially reveals the role of RBR in enforcing fate commitment over its role in cell-cycle control in this developmental context. Analysis of the phenotypes linked to the modulation of FAMA and RBR sheds new light on the way iterative divisions and terminal differentiation are coordinately regulated in a plant stem-cell lineage.
authors.affiliation: Department of Biology, Stanford University, Stanford, United States.
authors.forename: Juliana L
authors.identifier: http://orcid.org/0000-0001-9347-304X
authors.initials: JL
authors.lastname: Matos
authors.affiliation: Department of Biology, Stanford University, Stanford, United States.
authors.forename: On Sun
authors.identifier: http://orcid.org/0000-0002-8121-235X
authors.initials: OS
authors.lastname: Lau
authors.affiliation: Department of Biology, Stanford University, Stanford, United States.
authors.forename: Charles
authors.initials: C
authors.lastname: Hachez
authors.affiliation: Department of Molecular Genetics, Utrecht University, Utrecht, Netherlands.
authors.forename: Alfredo
authors.initials: A
authors.lastname: Cruz-Ramírez
authors.affiliation: Department of Molecular Genetics, Utrecht University, Utrecht, Netherlands.
authors.forename: Ben
authors.initials: B
authors.lastname: Scheres
authors.affiliation: Department of Biology, Stanford University, Stanford, United States.
authors.forename: Dominique C
authors.initials: DC
authors.lastname: Bergmann
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000166:cytology / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism; D051792:Basic Helix-Loop-Helix Proteins / Q000235:genetics* / Q000378:metabolism; D002453:Cell Cycle / Q000235:genetics; D002454:Cell Differentiation; D019070:Cell Lineage / Q000235:genetics*; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant; D008969:Molecular Sequence Data; D054046:Plant Stomata / Q000166:cytology / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D016415:Sequence Alignment
chemical_list: D029681:Arabidopsis Proteins; D051792:Basic Helix-Loop-Helix Proteins; C517481:FAMA protein, Arabidopsis; C487568:RBR1 protein, Arabidopsis
keywords: arabidopsis; bHLH transcription factor; differentiation; plant biology; retinoblastoma; stomata