title: pH-responsive nano carriers for doxorubicin delivery.
abstract: OBJECTIVE
  The aim of this study was to design stimuli-responsive nanocarriers for anti-cancer drug delivery. For this purpose, doxorubicin (DOX)-loaded, polysebacic anhydride (PSA) based nanocapsules (NC) were combined with pH-sensitive poly (L-histidine) (PLH).
  
  
  METHODS
  PSA nano-carriers were first loaded with DOX and were coated with poly L-histidine to introduce pH sensitivity. The PLH-coated NCs were then covered with polyethylene glycol (PEG) to reduce macrophage uptake. The drug release profile from this system was examined in two different buffer solutions prepared as acidic (pH5) and physiological (pH 7.4) media. The physical and chemical properties of the nanocapsules were characterized by Fourier transform infrared spectroscopy (FTIR), dynamic light scattering (DLS), ultraviolet and visible absorption spectroscopy (UV-VIS), and scanning electron microscopy (SEM). In vitro studies of the prepared nanocapsules were conducted in MDA-MB-231 breast cancer cells.
  
  
  RESULTS
  The results obtained by SEM and DLS revealed that nanocapsules have spherical morphology with an average size of 230 nm. Prepared pH sensitive nanocapsules exhibited pH-dependent drug release profile and promising intracellular release of drug. PEGylation of nanoparticles significantly prevented macrophage uptake compared to non-PEGylated particles.
authors.affiliation: Graduate Department of Polymer Science and Technology, Middle East Technical University, Ankara, Turkey.
authors.forename: Shahla
authors.initials: S
authors.lastname: Bagherifam
authors.forename: Frode Miltzow
authors.initials: FM
authors.lastname: Skjeldal
authors.forename: Gareth
authors.initials: G
authors.lastname: Griffiths
authors.forename: Gunhild M
authors.initials: GM
authors.lastname: Mælandsmo
authors.forename: Olav
authors.initials: O
authors.lastname: Engebråten
authors.forename: Bo
authors.initials: B
authors.lastname: Nyström
authors.forename: Vasif
authors.initials: V
authors.lastname: Hasirci
authors.forename: Nesrin
authors.initials: N
authors.lastname: Hasirci
mesh_terms: D000812:Anhydrides / Q000737:chemistry*; D000903:Antibiotics, Antineoplastic / Q000008:administration & dosage* / Q000494:pharmacology; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D003652:Decanoic Acids / Q000737:chemistry*; D004317:Doxorubicin / Q000008:administration & dosage* / Q000494:pharmacology; D004337:Drug Carriers / Q000737:chemistry*; D065546:Drug Liberation; D006639:Histidine / Q000737:chemistry; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism; D053769:Nanocapsules / Q000737:chemistry*; D010316:Particle Size; D011092:Polyethylene Glycols / Q000737:chemistry; D013499:Surface Properties
chemical_list: D000812:Anhydrides; D000903:Antibiotics, Antineoplastic; D003652:Decanoic Acids; D004337:Drug Carriers; D053769:Nanocapsules; C508111:Poly (sebacic anhydride); D011092:Polyethylene Glycols; D006639:Histidine; D004317:Doxorubicin