title: Genome-wide identification and tissue-specific expression analysis of UDP-glycosyltransferases genes confirm their abundance in Cicer arietinum (Chickpea) genome.
abstract: UDP-glycosyltransferases (EC 2.4.1.x; UGTs) are enzymes coded by an important gene family of higher plants. They are involved in the modification of secondary metabolites, phytohormones, and xenobiotics by transfer of sugar moieties from an activated nucleotide molecule to a wide range of acceptors. This modification regulates various functions like detoxification of xenobiotics, hormone homeostasis, and biosynthesis of secondary metabolites. Here, we describe the identification of 96 UGT genes in Cicer arietinum (CaUGT) and report their tissue-specific differential expression based on publically available RNA-seq and expressed sequence tag data. This analysis has established medium to high expression of 84 CaUGTs and low expression of 12 CaUGTs. We identified several closely related orthologs of CaUGTs in other genomes and compared their exon-intron arrangement. An attempt was made to assign functional specificity to chickpea UGTs by comparing substrate binding sites with experimentally determined specificity. These findings will assist in precise selection of candidate genes for various applications and understanding functional genomics of chickpea.
authors.affiliation: Division of Biochemical Sciences, CSIR-National Chemical Laboratory, Pune, Maharashtra, India.
authors.forename: Ranu
authors.initials: R
authors.lastname: Sharma
authors.affiliation: Department of Plant Developmental Biology, Max Planck Institute for Plant Breeding Research, Cologne, Germany.
authors.forename: Vimal
authors.initials: V
authors.lastname: Rawat
authors.affiliation: Division of Biochemical Sciences, CSIR-National Chemical Laboratory, Pune, Maharashtra, India.
authors.forename: C G
authors.initials: CG
authors.lastname: Suresh
mesh_terms: D000595:Amino Acid Sequence; D002874:Chromosome Mapping; D032461:Chromosomes, Plant / Q000737:chemistry*; D029863:Cicer / Q000145:classification / Q000201:enzymology / Q000235:genetics*; D005091:Exons; D020224:Expressed Sequence Tags; D018506:Gene Expression Regulation, Plant; D018745:Genome, Plant; D016695:Glycosyltransferases / Q000235:genetics* / Q000378:metabolism; D007438:Introns; D062105:Molecular Docking Simulation; D008969:Molecular Sequence Data; D009928:Organ Specificity; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D017423:Sequence Analysis, RNA
chemical_list: D010940:Plant Proteins; D016695:Glycosyltransferases