title: On-line solid-phase separation/preconcentration for the determination of copper in urine by flame atomic absorption spectrometry.
abstract: A new on-line separation/preconcentration system was developed for the determination of Cu(II) ions by flame atomic absorption spectrometry in urine samples. A newly synthesized chelating resin, by anchoring eriochrome blue black R reagent to Amberlite XAD-16 resin, was used as a packing material for the selective separation/preconcentration of Cu(II) ions. The influence of the parameters on the determination of Cu(II) ions such as pH of sample solution, amount of the resin, eluent type, interfering ions and flow variables was studied. The detection limit of the method was 1.0 μg L(-1) while precision was 2.3% (n=15) at 50 μg L(-1) Cu(II) level. The adsorption capacity of the resin was 217 μg g(-1) Cu(II). The accuracy of the method was proven using TMDA-64 standard lake water and synthetic urine sample. The developed method has been applied successfully to the determination of copper in urine with satisfactory results.
authors.affiliation: Erciyes University, Department of Chemistry, Faculty of Sciences, TR-38039 Kayseri, Turkey. Electronic address: sacmaci@erciyes.edu.tr.
authors.forename: Serife
authors.initials: S
authors.lastname: Saçmacı
authors.affiliation: Erciyes University, Department of Chemistry, Faculty of Sciences, TR-38039 Kayseri, Turkey.
authors.forename: Serkan
authors.initials: S
authors.lastname: Sahan
authors.affiliation: Erciyes University, Department of Chemistry, Faculty of Sciences, TR-38039 Kayseri, Turkey.
authors.forename: Uğur
authors.initials: U
authors.lastname: Sahin
authors.affiliation: Erciyes University, Department of Chemistry, Faculty of Sciences, TR-38039 Kayseri, Turkey.
authors.forename: Senol
authors.initials: S
authors.lastname: Kartal
authors.affiliation: Erciyes University, Department of Chemistry, Faculty of Sciences, TR-38039 Kayseri, Turkey.
authors.forename: Ahmet
authors.initials: A
authors.lastname: Ulgen
mesh_terms: D000327:Adsorption; D001391:Azo Compounds / Q000737:chemistry; D002614:Chelating Agents / Q000737:chemistry; D003300:Copper / Q000652:urine*; D005618:Fresh Water; D006801:Humans; D006863:Hydrogen-Ion Concentration; D011108:Polymers / Q000737:chemistry; D052616:Solid Phase Extraction; D013054:Spectrophotometry, Atomic; D014867:Water / Q000737:chemistry
chemical_list: D001391:Azo Compounds; D002614:Chelating Agents; D011108:Polymers; D014867:Water; C074337:Amberlite XAD-16; C039813:calcon; D003300:Copper
keywords: Chelating resin; Eriochrome blue black R; On-line preconcentration; Urinary copper