title: High expression of ROR2 in cancer cell correlates with unfavorable prognosis in colorectal cancer.
abstract: The receptor tyrosine kinase-like orphan receptor 2 (ROR2) is a transmembrane protein that belongs to a conserved family of tyrosine kinase receptors involved in several functional processes. ROR2 is overexpressed in various types of solid tumors; however, the expression of ROR2, as well as its functional and prognostic significance has yet to be evaluated in colorectal cancer (CRC). In this study, one-step quantitative reverse transcription-polymerase chain reaction and immunohistochemical analysis using tissue microarrays were used to evaluate ROR2 expression in CRC and to investigate the association between ROR2 expression and patient prognosis. We observed that the expression of ROR2 mRNA and protein was significantly higher in CRC specimens compared with normal, tumor-adjacent tissues (both p<0.05). Cytoplasmic ROR2 expression was related to TNM stage (p=0.041) and lymph node metastasis (N) (p=0.015). Kaplan-Meier and multivariate analyses suggested that high cytoplasmic ROR2 expression (p=0.001), poor tumor differentiation (p=0.001), and advanced TNM stage (p=0.001) and high preoperative CEA level (p<0.001) were significantly associated with unfavorable survival of CRC patients. These results suggest that ROR2 expression is correlated with malignant attributes of CRC and may serve as an indicator for poor prognosis in patients with CRC.
authors.affiliation: Department of General Surgery, Nantong University Affiliated Hospital, Nantong 226001, Jiangsu, China.
authors.forename: Haijun
authors.initials: H
authors.lastname: Mei
authors.affiliation: Department of Pathology, Nantong University Affiliated Hospital, Nantong 226001, Jiangsu, China.
authors.forename: Shuijin
authors.initials: S
authors.lastname: Lian
authors.affiliation: Department of Pathology, Nantong University Affiliated Hospital, Nantong 226001, Jiangsu, China.
authors.forename: Shu
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Department of Pathology, Nantong University Affiliated Hospital, Nantong 226001, Jiangsu, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.affiliation: Department of General Surgery, Nantong University Affiliated Hospital, Nantong 226001, Jiangsu, China. Electronic address: maoqsh@sina.com.
authors.forename: Qinsheng
authors.initials: Q
authors.lastname: Mao
authors.affiliation: Department of General Surgery, Nantong University Affiliated Hospital, Nantong 226001, Jiangsu, China. Electronic address: 13706292250@163.com.
authors.forename: Hua
authors.initials: H
authors.lastname: Wang
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000378:metabolism*; D018929:Cell Culture Techniques / Q000379:methods; D002681:China / Q000453:epidemiology; D015179:Colorectal Neoplasms / Q000378:metabolism* / Q000401:mortality* / Q000473:pathology; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009362:Neoplasm Metastasis; D009367:Neoplasm Staging; D015995:Prevalence; D011379:Prognosis; D057050:Receptor Tyrosine Kinase-like Orphan Receptors / Q000378:metabolism*; D015203:Reproducibility of Results; D012307:Risk Factors; D012680:Sensitivity and Specificity; D015996:Survival Rate; D055815:Young Adult
chemical_list: D014408:Biomarkers, Tumor; C078717:ROR2 protein, human; D057050:Receptor Tyrosine Kinase-like Orphan Receptors
keywords: CRC; Immunohistochemistry; Prognosis; ROR2; qPCR