title: [Quality assessment of Liuwei Wuling tablet by HPLC fingerprint and quantitative analysis].
abstract: Reversed phase high performance liquid chromatography with diode array detector was employed for simultaneous determination of six components and specific chromatograms analysis in Liuwei Wuling tablets with gradient elution of acetonitrile and water containing 0.1% phosphoric acid as mobile phase. The results showed that six components containing specnuezhenide, phillyrin, schisandrin, schisantherin A, schizandrin A and schizandrin B were separated well under the analytical condition. The average recoveries ranged from 98.96% to 100.5% with RSD less than 2. 0%. Twenty-five common peaks were selected as the specific chromatograms of Liuwei Wuling tablets with schisantherin A as the reference peak. Similarities calculated by cosine of angle, correlation coefficient and peak area ratio similarity (PAR) were all above 0. 95, indicating a good similarity between the reference and twenty batches of samples. Grubbs test and cluster analysis indicated that the established HPLC fingerprints and HPLC quantitative analysis can be used efficiently in the quality control of Liuwei Wuling tablets.
authors.forename: Hong-Ming
authors.initials: HM
authors.lastname: Liu
authors.forename: Nan-Nan
authors.initials: NN
authors.lastname: Xu
authors.forename: Lei
authors.initials: L
authors.lastname: Nie
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D011786:Quality Control; D013607:Tablets / Q000737:chemistry
chemical_list: D004365:Drugs, Chinese Herbal; D013607:Tablets