title: Characterization and quantification of the compounds of the ethanolic extract from Caesalpinia ferrea stem bark and evaluation of their mutagenic activity.
abstract: Caesalpinia ferrea Martius has traditionally been used in Brazil for many medicinal purposes, such as the treatment of bronchitis, diabetes and wounds. Despite its use as a medicinal plant, there is still no data regarding the genotoxic effect of the stem bark. This present work aims to assess the qualitative and quantitative profiles of the ethanolic extract from the stem bark of C. ferrea and to evaluate its mutagenic activity, using a Salmonella/microsome assay for this species. As a result, a total of twenty compounds were identified by Flow Injection Analysis Electrospray Ionization Ion Trap Mass Spectrometry (FIA-ESI-IT-MS/MSn) in the ethanolic extract from the stem bark of C. ferrea. Hydrolyzable tannins predominated, principally gallic acid derivatives. The HPLC-DAD method was developed for rapid quantification of six gallic acid compounds and ellagic acid derivatives. C. ferrea is widely used in Brazil, and the absence of any mutagenic effect in the Salmonella/microsome assay is important for pharmacological purposes and the safe use of this plant.
authors.affiliation: Organic Chemistry Department, Institute of Chemistry, São Paulo State University (UNESP), Araraquara 14800-900, Brazil.
authors.forename: Carlos César
authors.initials: CC
authors.lastname: Wyrepkowski
authors.affiliation: Organic Chemistry Department, Institute of Chemistry, São Paulo State University (UNESP), Araraquara 14800-900, Brazil.
authors.forename: Daryne Lu Maldonado Gomes da
authors.initials: DL
authors.lastname: Costa
authors.affiliation: Institute of Natural, Human, and Social Sciences, Mato Grosso Federal University (UFMT), Sinop 78557-267, Brazil.
authors.forename: Adilson Paulo
authors.initials: AP
authors.lastname: Sinhorin
authors.affiliation: Experimental Campus of São Vicente, São Paulo State University (UNESP), São Vicente 11350-000, Brazil.
authors.forename: Wagner
authors.initials: W
authors.lastname: Vilegas
authors.affiliation: Department of Biological Sciences, Faculty of Pharmaceutical Sciences, São Paulo State University (UNESP), Araraquara 14801-902, Brazil.
authors.forename: Rone Aparecido
authors.initials: RA
authors.lastname: De Grandis
authors.affiliation: Department of Biological Sciences, Faculty of Pharmaceutical Sciences, São Paulo State University (UNESP), Araraquara 14801-902, Brazil.
authors.forename: Flavia Aparecida
authors.initials: FA
authors.lastname: Resende
authors.affiliation: Department of Biological Sciences, Faculty of Pharmaceutical Sciences, São Paulo State University (UNESP), Araraquara 14801-902, Brazil.
authors.forename: Eliana Aparecida
authors.initials: EA
authors.lastname: Varanda
authors.affiliation: Organic Chemistry Department, Institute of Chemistry, São Paulo State University (UNESP), Araraquara 14800-900, Brazil. loursant@gmail.com.
authors.forename: Lourdes Campaner
authors.initials: LC
authors.lastname: dos Santos
mesh_terms: D029911:Caesalpinia / Q000737:chemistry*; D015394:Molecular Structure; D009153:Mutagens / Q000737:chemistry* / Q000494:pharmacology*; D024301:Plant Bark / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry* / Q000494:pharmacology*; D018547:Plant Stems / Q000737:chemistry*
chemical_list: D009153:Mutagens; D010936:Plant Extracts