title: Porous polylactic acid-silica hybrids: preparation, characterization, and study of mesenchymal stem cell osteogenic differentiation.
abstract: A novel approach to reinforce polymer porous membranes is presented. In the prepared hybrid materials, the inorganic phase of silica is synthesized in-situ and inside the pores of aminolyzed polylactic acid (PLA) membranes by sol-gel reactions using tetraethylorthosilicate (TEOS) and glycidoxypropyltrimethoxysilane (GPTMS) as precursors. The hybrid materials present a porous structure with a silica layer covering the walls of the pores while GPTMS serves also as coupling agent between the organic and inorganic phase. The adjustment of silica precursors ratio allows the modulation of the thermomechanical properties. Culture of mesenchymal stem cells on these supports in osteogenic medium shows the expression of characteristic osteoblastic markers and the mineralization of the extracellular matrix.
authors.affiliation: Physics Department, National Technical University of Athens, Zografou Campus, Athens, 15780, Greece; Centro de Biomateriales e Ingeniería Tisular, Universitat Politècnica de València, Camino de Vera s/n, Valencia, 46022, Spain. pandis@mail.ntua.gr.
authors.forename: Christos
authors.initials: C
authors.lastname: Pandis
authors.forename: Sara
authors.initials: S
authors.lastname: Trujillo
authors.forename: Joana
authors.initials: J
authors.lastname: Matos
authors.forename: Sara
authors.initials: S
authors.lastname: Madeira
authors.forename: Joaquín
authors.initials: J
authors.lastname: Ródenas-Rochina
authors.forename: Sotiria
authors.initials: S
authors.lastname: Kripotou
authors.forename: Apostolos
authors.initials: A
authors.lastname: Kyritsis
authors.forename: João F
authors.initials: JF
authors.lastname: Mano
authors.forename: José Luis
authors.initials: JL
authors.lastname: Gómez Ribelles
mesh_terms: D000818:Animals; D000880:Anthraquinones; D001391:Azo Compounds; D002454:Cell Differentiation / Q000502:physiology*; D002478:Cells, Cultured; D002850:Chromatography, Gel; D019344:Lactic Acid / Q000737:chemistry*; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D008855:Microscopy, Electron, Scanning; D010012:Osteogenesis / Q000502:physiology*; D044367:Phase Transition; D011091:Polyesters; D011108:Polymers / Q000737:chemistry*; D016062:Porosity; D012821:Silanes; D012822:Silicon Dioxide / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D013552:Swine; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D000880:Anthraquinones; D001391:Azo Compounds; D011091:Polyesters; D011108:Polymers; D012821:Silanes; C009798:C.I. direct red 80; D019344:Lactic Acid; C040733:tetraethoxysilane; C033616:poly(lactide); C010078:alizarin; D012822:Silicon Dioxide
keywords: organic-inorganic hybrid composites; porosity; proliferation and osteoblastic differentiation of cells; sol-gel processes; thermomechanical properties