title: Identification of putative fallopian tube stem cells.
abstract: Stem cells are used to repair and regenerate multiple tissues in the adult. We have previously shown that stem cells play a significant role in mediating endometrial repair and tissue regeneration. We hypothesized that the oviduct may possess a similar population of stem cells that contribute to the maintenance of this tissue. Here we identify label-retaining cells (LRCs) in the murine oviduct which indicate the presence of a stem/progenitor cell population in this tissue as well. Two-day-old CD-1 mice were injected intraperitoneally with 5-bromo-2-deoxyuridine (BrdU) or vehicle control. Female animals (n = 36 for each group) were killed at 6 weeks post injection. Reproductive tracts were removed, specimens were embedded in paraffin, and 5-µ sections were prepared. Oviduct was identified by hematoxylin and eosin staining and morphology. Immunofluorescence studies were performed on serial sections tissues (n = 12 per animal) using antibodies against BrdU. Confocal microscopy was used to identify 4',6-diamidino-2-phenylindole (DAPI)- and BrdU-stained nuclei. In the group of mice exposed to BrdU, we identified a population of LRCs in all specimens and not in controls. The putative stem cells are located at the base of each villi, suggesting the location of the stem cell niche. The number of DAPI-stained nuclei divided by the number of LRCs; LRCs constituted 0.5% of all nucleated cells. The oviduct contains a population of progenitor cells, likely used in the repair and regeneration of fallopian tube. Defective or insufficient stem cell reserve may underlie common tubal diseases, including hydrosalpinx and ectopic pregnancy.
authors.affiliation: Department of Obstetrics, Gynecology, and Reproductive Sciences, Yale University, New Haven, CT, USA Department of Obstetrics and Gynecology, Warren Alpert Medical School of Brown University, Providence, RI, USA.
authors.forename: Victoria
authors.initials: V
authors.lastname: Snegovskikh
authors.affiliation: Department of Obstetrics, Gynecology, and Reproductive Sciences, Yale University, New Haven, CT, USA.
authors.forename: Levent
authors.initials: L
authors.lastname: Mutlu
authors.affiliation: Department of Obstetrics, Gynecology, and Reproductive Sciences, Yale University, New Haven, CT, USA.
authors.forename: Effi
authors.initials: E
authors.lastname: Massasa
authors.affiliation: Department of Obstetrics, Gynecology, and Reproductive Sciences, Yale University, New Haven, CT, USA hugh.taylor@yale.edu.
authors.forename: Hugh S
authors.initials: HS
authors.lastname: Taylor
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001973:Bromodeoxyuridine; D049109:Cell Proliferation; D005187:Fallopian Tubes / Q000166:cytology*; D005260:Female; D005455:Fluorescent Antibody Technique; D051379:Mice; D008856:Microscopy, Fluorescence; D055153:Stem Cell Niche; D013234:Stem Cells / Q000502:physiology*
chemical_list: D001973:Bromodeoxyuridine
keywords: ectopic pregnancy; fallopian tube; hydrosalpinx; infertility; ovarian cancer; oviduct; reproduction; reproductive tract; stem cell