title: The design of dextran-based hypoxia-inducible hydrogels via in situ oxygen-consuming reaction.
abstract: Hypoxia plays a critical role in the development and wound healing process, as well as a number of pathological conditions. Here, dextran-based hypoxia-inducible (Dex-HI) hydrogels formed with in situ oxygen consumption via a laccase-medicated reaction are reported. Oxygen levels and gradients were accurately predicted by mathematical simulation. It is demonstrated that Dex-HI hydrogels provide prolonged hypoxic conditions up to 12 h. The Dex-HI hydrogel offers an innovative approach to delineate not only the mechanism by which hypoxia regulates cellular responses, but may facilitate the discovery of new pathways involved in the generation of hypoxic and oxygen gradient environments.
authors.affiliation: Department of Chemical and Biomolecular Engineering, Johns Hopkins Physical Sciences-Oncology Center and Institute for NanoBioTechnology, Johns Hopkins University, Baltimore, MD, 21218, USA.
authors.forename: Kyung Min
authors.initials: KM
authors.lastname: Park
authors.forename: Michael R
authors.initials: MR
authors.lastname: Blatchley
authors.forename: Sharon
authors.initials: S
authors.lastname: Gerecht
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry / Q000378:metabolism; D003911:Dextrans / Q000737:chemistry / Q000378:metabolism*; D020100:Hydrogels / Q000737:chemistry / Q000378:metabolism*; D027802:Hypoxis / Q000737:chemistry / Q000378:metabolism*; D042845:Laccase / Q000737:chemistry / Q000378:metabolism; D015394:Molecular Structure; D010100:Oxygen / Q000737:chemistry / Q000378:metabolism*; D010101:Oxygen Consumption
chemical_list: D001672:Biocompatible Materials; D003911:Dextrans; D020100:Hydrogels; D042845:Laccase; D010100:Oxygen
keywords: dextran; hypoxia; injectable hydrogels; laccase-mediated cross-linking reactions; oxygen controllable hydrogels