title: Evaluation of CellSolutions BestPrep® automated thin-layer liquid-based cytology Papanicolaou slide preparation and BestCyte® cell sorter imaging system.
abstract: OBJECTIVE
  A double-blind study was conducted to compare the performance of the new BestPrep® (CellSolutions) liquid-based thin-layer Papanicolaou (Pap) test with ThinPrep® (Hologic).
  
  
  METHODS
  Samples from the study patients (n = 105) were collected twice in the same encounter with the ThinPrep sample always taken first and the BestPrep sample collected second. Slides were prepared according to both manufacturers' protocols and evaluated using manual microscopic review and the BestCyte® cell sorter imaging system (CellSolutions). Diagnostic truth for each case was determined by independent manual review of both slides by multiple pathologists and histology when available. The presence of atypical squamous cells of undetermined significance was the threshold for positive for sensitivity and specificity calculations.
  
  
  RESULTS
  BestPrep and ThinPrep, by manual review, had sensitivities for high-grade squamous intraepithelial lesion (HSIL) cases of 100 and 95.6%, respectively. Using the BestCyte cell sorter, both had 100% sensitivity. For the same HSIL cases, the digene HC2 high-risk human papillomavirus DNA test had sensitivities of 100% (BestPrep) and 95.6% (ThinPrep). Specificities were 71.4% (BestPrep) and 54.8% (ThinPrep).
  
  
  CONCLUSIONS
  BestPrep was equivalent to ThinPrep for manual review even though BestPrep was always the second sample collected. The BestCyte cell sorter provides a practical alternative to manual review for both BestPrep and ThinPrep slides.
authors.affiliation: Department of Pathology, CHU Sart Tilman, University of Liège, Liège, Belgium.
authors.forename: Agnes
authors.initials: A
authors.lastname: Delga
authors.forename: Frederic
authors.initials: F
authors.lastname: Goffin
authors.forename: Frederic
authors.initials: F
authors.lastname: Kridelka
authors.forename: Raphaël
authors.initials: R
authors.lastname: Marée
authors.forename: Chantal
authors.initials: C
authors.lastname: Lambert
authors.forename: Philippe
authors.initials: P
authors.lastname: Delvenne
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D057205:Automation, Laboratory; D002294:Carcinoma, Squamous Cell / Q000150:complications / Q000175:diagnosis* / Q000473:pathology; D004311:Double-Blind Method; D005260:Female; D006801:Humans; D007090:Image Interpretation, Computer-Assisted; D008403:Mass Screening; D008875:Middle Aged; D065006:Papanicolaou Test / Q000295:instrumentation* / Q000379:methods; D027383:Papillomaviridae / Q000502:physiology; D030361:Papillomavirus Infections / Q000150:complications / Q000175:diagnosis* / Q000473:pathology; D012212:Rheology; D013048:Specimen Handling; D002583:Uterine Cervical Neoplasms / Q000150:complications / Q000175:diagnosis* / Q000473:pathology; D014626:Vaginal Smears / Q000379:methods / Q000706:statistics & numerical data*; D002578:Uterine Cervical Dysplasia / Q000150:complications / Q000175:diagnosis* / Q000473:pathology