title: Effects of digoxin and Na, K-ATPase immunoexpression on human oral squamous carcinomas.
abstract: OBJECTIVE
  The present study evaluated the expression of α1 and β1 Na,K-ATPase, as well as the effects of digoxin (DGX) on oral squamous cell carcinomas (OSCCs).
  
  
  METHODS
  Immunohistochemical expression of α1 and β1 Na,K-ATPase were evaluated in 60 patients who underwent treatment at the São João de Deus Hospital. SCC-25 viability was assessed by the colorimetric assay. Chi-square or Fisher's exact tests were used to analyze the association of α1 and β1 Na,K-ATPase expression with the variables.
  
  
  RESULTS
  Immunoexpression of α1 and β1 Na,K-ATPase were observed in 28% and 55% of the tumors, however these proteins were not significant prognostic factors. Tobacco was significantly associated with α1 expression. SCC-25 viability decreased significantly after treatment with 1 μM DGX at 24 h.
  
  
  CONCLUSIONS
  The smoking status of OSCC patients was significantly associated with α1 expression and DGX affected the SCC-25 viability in a dose- and duration-dependent manner.
authors.affiliation: Laboratório de Bioquímica Celular, Faculdade de Bioquímica, Universidade Federal de São João Del Rei, Campus Centro-Oeste Dona Lindu, Divinópolis, Minas Gerais, Brazil Hospital São João de Deus, Divinópolis, Minas Gerais, Brazil.
authors.forename: Aline Lauda Freitas
authors.initials: AL
authors.lastname: Chaves
authors.affiliation: Laboratório de Processamento de Tecidos e Biologia Experimental, Universidade Federal de São João Del Rei, Campus Centro-Oeste Dona Lindu, Divinópolis, Minas Gerais, Brazil.
authors.forename: Priscila Oliveira
authors.initials: PO
authors.lastname: De Lima
authors.affiliation: Faculdade de Medicina, Universidade Federal de São João del Rei, Campus Centro-Oeste Dona Lindu, Divinópolis, Minas Gerais, Brazil Hospital São João de Deus, Divinópolis, Minas Gerais, Brazil.
authors.forename: João Marcos Arantes
authors.initials: JM
authors.lastname: Soares
authors.affiliation: Laboratório de Processamento de Tecidos e Biologia Experimental, Universidade Federal de São João Del Rei, Campus Centro-Oeste Dona Lindu, Divinópolis, Minas Gerais, Brazil.
authors.forename: Hélio Batista
authors.initials: HB
authors.lastname: Dos Santos
authors.affiliation: Laboratório de Patologia Experimental, Universidade Federal de São João Del Rei, Campus Centro-Oeste Dona Lindu, Divinópolis, Minas Gerais, Brazil.
authors.forename: Ana Gabriela
authors.initials: AG
authors.lastname: Silva
authors.affiliation: Hospital São João de Deus, Divinópolis, Minas Gerais, Brazil Laboratório de Patologia Experimental, Universidade Federal de São João Del Rei, Campus Centro-Oeste Dona Lindu, Divinópolis, Minas Gerais, Brazil.
authors.forename: Luciana Vieira
authors.initials: LV
authors.lastname: Muniz
authors.affiliation: Laboratório de Biologia Celular e Mutagênese, Universidade Federal de São João del Rei, Campus Centro-Oeste Dona Lindu, Divinópolis, Minas Gerais, Brazil.
authors.forename: Fábio Vieira
authors.initials: FV
authors.lastname: Dos Santos
authors.affiliation: Laboratório de Processamento de Tecidos e Biologia Experimental, Universidade Federal de São João Del Rei, Campus Centro-Oeste Dona Lindu, Divinópolis, Minas Gerais, Brazil.
authors.forename: Michele Conceição
authors.initials: MC
authors.lastname: Pereira
authors.affiliation: Laboratório de Patologia Experimental, Universidade Federal de São João Del Rei, Campus Centro-Oeste Dona Lindu, Divinópolis, Minas Gerais, Brazil leaugust@yahoo.com.br rosyiara@gmail.com.
authors.forename: Rosy Iara Maciel
authors.initials: RI
authors.lastname: De Azambuja Ribeiro
authors.affiliation: Laboratório de Bioquímica Celular, Faculdade de Bioquímica, Universidade Federal de São João Del Rei, Campus Centro-Oeste Dona Lindu, Divinópolis, Minas Gerais, Brazil leaugust@yahoo.com.br rosyiara@gmail.com.
authors.forename: Leandro Augusto
authors.initials: LA
authors.lastname: Barbosa
mesh_terms: D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000378:metabolism* / Q000401:mortality / Q000473:pathology; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects / Q000235:genetics; D004077:Digoxin / Q000494:pharmacology*; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D006801:Humans; D009062:Mouth Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000401:mortality / Q000473:pathology; D060787:Neoplasm Grading; D009367:Neoplasm Staging; D021122:Protein Subunits / Q000235:genetics / Q000378:metabolism; D012307:Risk Factors; D000254:Sodium-Potassium-Exchanging ATPase / Q000235:genetics / Q000378:metabolism*
chemical_list: D004791:Enzyme Inhibitors; D021122:Protein Subunits; D004077:Digoxin; D000254:Sodium-Potassium-Exchanging ATPase
keywords: Na,K-ATPase; Oral squamous cell carcinoma; cell viability; digoxin; prognosis