title: A direct competitive assay-based aptasensor for sensitive determination of tetracycline residue in honey.
abstract: Tetracycline (TC) is a common antibacterial agent used for prevention and control of animal diseases. The increasing concern about TC residue in food demands high-performing analytical techniques for food quality assessment. Biosensors represent a promising tool for food safety analysis as they can fulfill some demand that the conventional methods do not attain. In this study, a novel colorimetric aptasensor was developed for sensitive detection of TC in honey. The aptasensor was based on a modified direct competitive enzyme-linked aptamer assay (dc-ELAA) scheme utilizing a 76 mer single-stranded DNA (ssDNA) aptamer selected by Systematic Evolution of Ligands by Exponential Enrichment (SELEX). The optimized aptasensor showed a good limit of detection (LOD of 0.0978 ng/mL), a wide linear range (0.1-1000 ng/mL) toward TC in honey, with good recoveries (92.09-109.7%) in TC-spiked honey, and was compared with an indirect competitive assay-based aptasensor and validated with a standard ELISA. The biosensor based on dc-ELAA with good limit of detection and simplicity can be applied for high-throughput detection of TC in food.
authors.affiliation: Beijing Key Laboratory of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, P.R. China.
authors.forename: Sai
authors.initials: S
authors.lastname: Wang
authors.affiliation: Beijing Key Laboratory of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, P.R. China.
authors.forename: Jiahui
authors.initials: J
authors.lastname: Liu
authors.affiliation: Institute of Food Safety, Chinese Academy of Inspection and Quarantine, Beijing 100123, P.R. China.
authors.forename: Wei
authors.initials: W
authors.lastname: Yong
authors.affiliation: Beijing Key Laboratory of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, P.R. China.
authors.forename: Qilong
authors.initials: Q
authors.lastname: Chen
authors.affiliation: Beijing Key Laboratory of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, P.R. China.
authors.forename: Liya
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Beijing Key Laboratory of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, P.R. China. Electronic address: yydong@mail.buct.edu.cn.
authors.forename: Yiyang
authors.initials: Y
authors.lastname: Dong
authors.affiliation: Beijing Key Laboratory of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, P.R. China.
authors.forename: Haijia
authors.initials: H
authors.lastname: Su
authors.affiliation: Beijing Key Laboratory of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, P.R. China.
authors.forename: Tianwei
authors.initials: T
authors.lastname: Tan
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry*; D015374:Biosensing Techniques / Q000379:methods*; D004277:DNA, Single-Stranded / Q000737:chemistry*; D004797:Enzyme-Linked Immunosorbent Assay; D006722:Honey / Q000032:analysis*; D057230:Limit of Detection; D013752:Tetracycline / Q000032:analysis*
chemical_list: D052157:Aptamers, Nucleotide; D004277:DNA, Single-Stranded; D013752:Tetracycline
keywords: Aptasensor; Direct competitive enzyme-linked aptamer assay; Honey; Tetracycline