title: Effect ofHyssopus officinalisL. on inhibiting airway inflammation and immune regulation in a chronic asthmatic mouse model.
abstract: The Uygur herb,Hyssopus officinalisL.,has been demonstrated to affect the levels of a number of cytokines in asthmatic mice, including interleukin-4, -6 and -17 and interferon-γ. In the present study, the effect ofHyssopus officinalisL. on airway immune regulation and airway inflammation was investigated in a mouse model of chronic asthma. A total of 32 BALB/c mice were randomly divided into four groups, which included the normal, chronic asthmatic, dexamethasone treatment andHyssopus officinalisL.treatment groups. Mice were sensitized and challenged with ovalbumin to establish an asthma model and the ratio of eosinophils (EOS) in the bronchoalveolar lavage fluid (BALF) was determined. In addition, the levels of immunoglobulin (Ig)E and IgG were detected using an enzyme-linked immunosorbent assay. The degree of airway mucus secretion was observed using the periodic acid-Schiff stain method. The results demonstrated that the ratio of EOS in the BALF and the level of serum IgE in the chronic asthmatic and dexamethasone treatment groups increased, while the level of serum IgG decreased, when compared with the normal group. In addition, excessive secretion of airway mucus was observed in these two groups. However, the EOS ratio in the BALF and the levels of serum IgE and IgG in theHyssopus officinalisL. treatment group were similar to the results observed in the normal group. In conclusion,Hyssopus officinalisL. not only plays an anti-inflammatory role by inhibiting the invasion of EOS and decreasing the levels of IgE, but also affects immune regulation.
authors.affiliation: Xinjiang National Clinical Research Base of Traditional Chinese Medicine, Xinjiang Medical University, Ürümqi, Xinjiang 830011, P.R. China ; Department of Immunology, College of Basic Medicine, Ürümqi, Xinjiang 830011, P.R. China.
authors.forename: Xiaojuan
authors.initials: X
authors.lastname: Ma
authors.affiliation: Department of Clinical Laboratory, First Affiliated Hospital of Xinjiang Medical University, Ürümqi, Xinjiang 830054, P.R. China.
authors.forename: Xiumin
authors.initials: X
authors.lastname: Ma
authors.affiliation: Ürümqi General Hospital of Lanzhou Military Area Command, Ürümqi, Xinjiang 830000, P.R. China.
authors.forename: Zhixing
authors.initials: Z
authors.lastname: Ma
authors.affiliation: Xinjiang National Clinical Research Base of Traditional Chinese Medicine, Xinjiang Medical University, Ürümqi, Xinjiang 830011, P.R. China ; Department of Immunology, College of Basic Medicine, Ürümqi, Xinjiang 830011, P.R. China.
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Immunology, College of Basic Medicine, Ürümqi, Xinjiang 830011, P.R. China.
authors.forename: Zhan
authors.initials: Z
authors.lastname: Sun
authors.affiliation: Department of Immunology, College of Basic Medicine, Ürümqi, Xinjiang 830011, P.R. China.
authors.forename: Wenyan
authors.initials: W
authors.lastname: Yu
authors.affiliation: Xinjiang National Clinical Research Base of Traditional Chinese Medicine, Xinjiang Medical University, Ürümqi, Xinjiang 830011, P.R. China.
authors.forename: Fengsen
authors.initials: F
authors.lastname: Li
authors.affiliation: Department of Immunology, College of Basic Medicine, Ürümqi, Xinjiang 830011, P.R. China.
authors.forename: Jianbing
authors.initials: J
authors.lastname: Ding
keywords: Hyssopus officinalis L.; airway inflammation; chronic asthma; immune regulation