title: Xanthomonas oryzae pv. oryzae RpfE Regulates Virulence and Carbon Source Utilization without Change of the DSF Production.
abstract: It has been known that most regulation of pathogenicity factor (rpf) genes in xanthomonads regulates virulence in response to the diffusible signal factor, DSF. Although many rpf genes have been functionally characterized, the function of rpfE is still unknown. We cloned the rpfE gene from a Xanthomonas oryzae pv. oryzae (Xoo) Korean race KACC10859 and generated mutant strains to elucidate the role of RpfE with respect to the rpf system. Through experiments using the rpfE-deficient mutant strain, we found that mutation in rpfE gene in Xoo reduced virulence, swarm motility, and production of virulence factors such as cellulase and extracellular polysaccharide. Disease progress by the rpfE-deficient mutant strain was significantly slowed compared to disease progress by the wild type and the number of the rpfE-deficient mutant strain was lower than that of the wild type in the early phase of infection in the inoculated rice leaf. The rpfE mutant strain was unable to utilize sucrose or xylose as carbon sources efficiently in culture. The mutation in rpfE, however, did not affect DSF synthesis. Our results suggest that the rpfE gene regulates the virulence of Xoo under different nutrient conditions without change of DSF production.
authors.affiliation: Department of Plant Medicine, Chungbuk National University, Cheongju, Chungbuk 361-763, Korea.
authors.forename: Jung-Hee
authors.initials: JH
authors.lastname: Cho
authors.affiliation: Department of Plant Molecular Systems Biotech & Crop Biotech Institute, KyungHee University, Yongin 446-701, Korea.
authors.forename: Joo-Mi
authors.initials: JM
authors.lastname: Yoon
authors.affiliation: Department of Plant Molecular Systems Biotech & Crop Biotech Institute, KyungHee University, Yongin 446-701, Korea.
authors.forename: Sang-Won
authors.initials: SW
authors.lastname: Lee
authors.affiliation: Department of Plant Medicine, Chungbuk National University, Cheongju, Chungbuk 361-763, Korea.
authors.forename: Young-Hee
authors.initials: YH
authors.lastname: Noh
authors.affiliation: Department of Plant Medicine, Chungbuk National University, Cheongju, Chungbuk 361-763, Korea.
authors.forename: Jae-Soon
authors.initials: JS
authors.lastname: Cha
keywords: carbon source utilization; cellulase; diffusible signal factor; rpfE; virulence regulation