title: Interferon regulatory factor 7 is involved in the growth of Epstein-Barr virus-transformed human B lymphocytes.
abstract: The cellular interferon (IFN) regulatory factor-7 (IRF7) is closely associated with the Epstein-Barr virus (EBV)-mediated transformation of B lymphocytes in vitro and in vivo. However, the exact role of IRF7 in viral transformation is not clear. We have found that knockdown of IRF7 leads to growth inhibition of EBV-transformed cells, and restoration of IRF7 by exogenous plasmid correlates with growth recovery of the viral transformed cells. In addition, IRF7-knockdown cells have a lower proliferation but a higher apoptotic rate than control cells. Moreover, reduction of IRF7 leads to reduction of major viral oncoprotein, latent membrane protein 1 (LMP1). Our data suggest that IRF7 may be a factor in EBV transformation and a useful target in the therapy of EBV-mediated neoplasia.
authors.affiliation: Center for Neuroimmunology and Regenerative Therapy, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai, PR China; State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan 430072, PR China; Nebraska Center for Virology, School of Biological Sciences, University of Nebraska, Lincoln, Nebraska 68588, United States. Electronic address: dxu0927@gmail.com.
authors.forename: Dongsheng
authors.initials: D
authors.lastname: Xu
authors.affiliation: Center for Neuroimmunology and Regenerative Therapy, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai, PR China.
authors.forename: Yanyan
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Institute for Molecular Virology, Saint Louis University Health Sciences Center, St. Louis, Missouri 63104, United States.
authors.forename: Lingjun
authors.initials: L
authors.lastname: Zhao
authors.affiliation: Nebraska Center for Virology, School of Biological Sciences, University of Nebraska, Lincoln, Nebraska 68588, United States.
authors.forename: Mingxia
authors.initials: M
authors.lastname: Cao
authors.affiliation: Nebraska Center for Virology, School of Biological Sciences, University of Nebraska, Lincoln, Nebraska 68588, United States.
authors.forename: Amy
authors.initials: A
authors.lastname: Lingel
authors.affiliation: Nebraska Center for Virology, School of Biological Sciences, University of Nebraska, Lincoln, Nebraska 68588, United States. Electronic address: lzhang2@unl.edu.
authors.forename: Luwen
authors.initials: L
authors.lastname: Zhang
mesh_terms: D001402:B-Lymphocytes / Q000502:physiology* / Q000821:virology*; D049109:Cell Proliferation; D002472:Cell Transformation, Viral; D055785:Gene Knockdown Techniques; D005816:Genetic Complementation Test; D004854:Herpesvirus 4, Human / Q000502:physiology*; D006801:Humans; D050839:Interferon Regulatory Factor-7 / Q000235:genetics / Q000378:metabolism*; D014763:Viral Matrix Proteins / Q000378:metabolism
chemical_list: C037662:EBV-associated membrane antigen, Epstein-Barr virus; C494234:IRF7 protein, human; D050839:Interferon Regulatory Factor-7; D014763:Viral Matrix Proteins
keywords: EBV; IRF7; LMP1; Lymphoma; Transformation