title: Streptomyces vulcanius sp. nov., a novel actinomycete isolated from volcanic sediment.
abstract: A novel actinomycete, designated strain NEAU-C3(T), was isolated from volcanic sediment collected from Longwan, Jilin province, north China and characterized using a polyphasic approach. The organism was found to have morphological and chemotaxonomic characteristics typical of the members of the genus Streptomyces. EzTaxon-e analysis of the 16S rRNA gene sequence indicated strain NEAU-C3(T) to be most closely related to Streptomyces hygroscopicus subsp. ossamyceticus JCM 4965(T) (97.7 % sequence similarity) and Streptomyces torulosus JCM 4872(T) (97.7 %). Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain NEAU-C3(T) belongs within the genus Streptomyces and forms a separate subclade, an association that was supported by a bootstrap value of 72 % in the neighbour-joining tree and also recovered with the maximum-likelihood algorithm. The DNA-DNA hybridization values between strain NEAU-C3(T) and the two most closely related type strains were low enough to justify the assignment of the strain to a novel species. On the basis of these phenotypic, phylogenetic and chemotaxonomic characteristics, it is concluded that strain NEAU-C3(T) represents a novel species of the genus Streptomyces, for which the name Streptomyces vulcanius sp. nov. is proposed. The type strain is NEAU-C3(T) (=CGMCC 4.7177(T)=DSM 42139(T)).
authors.affiliation: Key Laboratory of Agriculture Biological Functional Gene of Heilongjiang Provincial Education Committee, Northeast Agricultural University, No. 59 Mucai Street, Xiangfang District, Harbin, 150030, People's Republic of China.
authors.forename: Feiyu
authors.initials: F
authors.lastname: Jia
authors.forename: Chongxi
authors.initials: C
authors.lastname: Liu
authors.forename: Junwei
authors.initials: J
authors.lastname: Zhao
authors.forename: Yuejing
authors.initials: Y
authors.lastname: Zhang
authors.forename: Lianjie
authors.initials: L
authors.lastname: Li
authors.forename: Shuyu
authors.initials: S
authors.lastname: Zhou
authors.forename: Yue
authors.initials: Y
authors.lastname: Shen
authors.forename: Xiangjing
authors.initials: X
authors.lastname: Wang
authors.forename: Wensheng
authors.initials: W
authors.lastname: Xiang
mesh_terms: D015373:Bacterial Typing Techniques; D002241:Carbohydrates / Q000032:analysis; D002473:Cell Wall / Q000737:chemistry; D002681:China; D002851:Chromatography, High Pressure Liquid; D016000:Cluster Analysis; D003600:Cytosol / Q000737:chemistry; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D008401:Gas Chromatography-Mass Spectrometry; D019015:Geologic Sediments / Q000382:microbiology*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010457:Peptidoglycan / Q000032:analysis; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013302:Streptomyces / Q000737:chemistry / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D024482:Vitamin K 2 / Q000032:analysis
chemical_list: D002241:Carbohydrates; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D010457:Peptidoglycan; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2