title: Encapsulation of anticancer drug by hydrogen-bonded multilayers of tannic acid.
abstract: Tannic acid (TA)-based multilayer assemblies have attracted increasing interest for biomedical applications. Here we explore properties of TA-poly(N-vinylpyrrolidone) (TA-PVPON) hydrogen-bonded multilayers for drug encapsulation and long-term storage. We demonstrate that the small molecular weight anticancer drug, doxorubicin (DOX), can be successfully loaded into (TA-PVPON) capsules with high encapsulation efficiency. We have also found that the encapsulated DOX can be efficiently stored inside the capsules for the pH range from pH = 7.4 to pH = 5. We show that the chemical and functional stability of TA at neutral and basic pH values is achieved through complexation with PVPON. The UV-vis spectrophotometry and in situ ellipsometry analyses of the hydrogen bonding interactions between TA and PVPON at different pH values reveal pH-dependent behavior of TA-PVPON capsules for the pH range from pH = 7.4 to pH = 5. Increasing deposition pH value from pH = 5 to pH = 7.4 leads to a 2-fold decrease in capsule thickness. However, this trend is reversed when salt concentration of the deposition solutions is increased from 0.01 M to 0.1 M NaCl. We have also demonstrated that the permeability of (TA-PVPON) capsules prepared using low salt deposition conditions and pH = 7.4 can be increased 2-fold by exposure of the capsules to 0.1 M NaCl salt solutions at the same pH. Our work opens new perspectives for design of novel polymer carriers for controlled drug delivery in cancer therapy.
authors.affiliation: Department of Chemistry, University of Alabama at Birmingham, 901 14th St South, CHEM294, Birmingham, AL 35294, USA. ekharlam@uab.edu.
authors.forename: Fei
authors.initials: F
authors.lastname: Liu
authors.forename: Veronika
authors.initials: V
authors.lastname: Kozlovskaya
authors.forename: Oleksandra
authors.initials: O
authors.lastname: Zavgorodnya
authors.forename: Claudia
authors.initials: C
authors.lastname: Martinez-Lopez
authors.forename: Shane
authors.initials: S
authors.lastname: Catledge
authors.forename: Eugenia
authors.initials: E
authors.lastname: Kharlampieva
mesh_terms: D000903:Antibiotics, Antineoplastic / Q000737:chemistry*; D004317:Doxorubicin / Q000737:chemistry; D004337:Drug Carriers / Q000737:chemistry*; D016650:Fluorescein-5-isothiocyanate / Q000737:chemistry; D036681:Fluorescence Recovery After Photobleaching; D006859:Hydrogen / Q000737:chemistry*; D006860:Hydrogen Bonding; D006863:Hydrogen-Ion Concentration; D018613:Microscopy, Confocal; D009994:Osmolar Concentration; D010539:Permeability; D011205:Povidone / Q000737:chemistry; D013634:Tannins / Q000737:chemistry*
chemical_list: D000903:Antibiotics, Antineoplastic; D004337:Drug Carriers; D013634:Tannins; D006859:Hydrogen; D004317:Doxorubicin; D011205:Povidone; D016650:Fluorescein-5-isothiocyanate