title: Nucleoside triphosphate diphosphohydrolase1 (TcNTPDase-1) gene expression is increased due to heat shock and in infective forms of Trypanosoma cruzi.
abstract: BACKGROUND
  Ecto-Nucleoside Triphosphate Diphosphohydrolases (Ecto-NTPDases) are enzymes that hydrolyze tri- and/or di-phosphate nucleotides. Evidences point to their participation in Trypanosoma cruzi virulence and infectivity. In this work, we evaluate TcNTPDase-1 gene expression in comparison with ecto-NTPDase activity, in order to study the role of TcNTPDase-1 in parasite virulence, infectivity and adaptation to heat shock.
  
  
  RESULTS
  Comparison between distinct T. cruzi isolates (Y, 3663 and 4167 strains, and Dm28c, LL014 and CL-14 clones) showed that TcNTPDase-1 expression was 7.2 ± 1.5 times higher in the Dm28c than the CL-14 avirulent clone. A remarkable expression increase was also observed in the trypomastigote and amastigote forms (22.5 ± 5.6 and 16.3 ± 3.8 times higher than epimastigotes, respectively), indicating that TcNTPDase-1 is overexpressed in T. cruzi infective forms. Moreover, heat shock and long-term cultivation also induced a significant increment on TcNTPDase-1 expression.
  
  
  CONCLUSIONS
  Our results suggest that TcNTPDase-1 plays an important role on T. cruzi infectivity and adaptation to stress conditions, such as long-term cultivation and heat shock.
authors.affiliation: Laboratorio de Biologia Molecular e Doenças Endêmicas, Instituto Oswaldo Cruz/ FIOCRUZ, Av. Brasil, 4365. Pavilhão Leônidas Deane, sala 209. Manguinhos, Rio de Janeiro, Brazil. natalia.lins@ioc.fiocruz.br.
authors.forename: Natália Lins
authors.initials: NL
authors.lastname: Silva-Gomes
authors.affiliation: Laboratorio de Biologia Molecular e Doenças Endêmicas, Instituto Oswaldo Cruz/ FIOCRUZ, Av. Brasil, 4365. Pavilhão Leônidas Deane, sala 209. Manguinhos, Rio de Janeiro, Brazil. vidal@ioc.fiocruz.br.
authors.forename: Vitor
authors.initials: V
authors.lastname: Ennes-Vidal
authors.affiliation: Laboratorio de Biologia Molecular e Doenças Endêmicas, Instituto Oswaldo Cruz/ FIOCRUZ, Av. Brasil, 4365. Pavilhão Leônidas Deane, sala 209. Manguinhos, Rio de Janeiro, Brazil. julliane.castro@ioc.fiocruz.br.
authors.forename: Julliane Castro Ferreira
authors.initials: JC
authors.lastname: Carolo
authors.affiliation: Laboratório de Biologia Celular, Instituto Oswaldo Cruz/ FIOCRUZ, Rio de Janeiro, Brazil. meusermb@ioc.fiocruz.br.
authors.forename: Marcos Meuser
authors.initials: MM
authors.lastname: Batista
authors.affiliation: Laboratório de Biologia Celular, Instituto Oswaldo Cruz/ FIOCRUZ, Rio de Janeiro, Brazil. soeiro@ioc.fiocruz.br.
authors.forename: Maria Nazaré
authors.initials: MN
authors.lastname: Soeiro
authors.affiliation: Laboratório de Biologia Celular, Instituto Oswaldo Cruz/ FIOCRUZ, Rio de Janeiro, Brazil. rubemb@ioc.fiocruz.br.
authors.forename: Rubem
authors.initials: R
authors.lastname: Menna-Barreto
authors.affiliation: Laboratorio de Biologia Molecular e Doenças Endêmicas, Instituto Oswaldo Cruz/ FIOCRUZ, Av. Brasil, 4365. Pavilhão Leônidas Deane, sala 209. Manguinhos, Rio de Janeiro, Brazil. otacilio@ioc.fiocruz.br.
authors.forename: Otacilio Cruz
authors.initials: OC
authors.lastname: Moreira
mesh_terms: D001483:Base Sequence; D016054:DNA, Protozoan / Q000235:genetics; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology*; D018869:Heat-Shock Response; D008969:Molecular Sequence Data; D043583:Nucleoside-Triphosphatase / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013997:Time Factors; D014349:Trypanosoma cruzi / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism
chemical_list: D016054:DNA, Protozoan; D012333:RNA, Messenger; D043583:Nucleoside-Triphosphatase