title: Establishment of a Cre recombinase based mutagenesis protocol for markerless gene deletion in Streptococcus suis.
abstract: The lack of knowledge about pathogenicity mechanisms of Streptococcus (S.) suis is, at least partially, attributed to limited methods for its genetic manipulation. Here, we established a Cre-lox based recombination system for markerless gene deletions in S. suis serotype 2 with high selective pressure and without undesired side effects.
authors.affiliation: Institute for Microbiology, University of Veterinary Medicine Hannover, Hannover, Germany.
authors.forename: A
authors.initials: A
authors.lastname: Koczula
authors.affiliation: Institute for Microbiology, University of Veterinary Medicine Hannover, Hannover, Germany. Electronic address: joerg.willenborg@tiho-hannover.de.
authors.forename: J
authors.initials: J
authors.lastname: Willenborg
authors.affiliation: Department of Microbial Genetics, University of Tuebingen, Tuebingen, Germany.
authors.forename: R
authors.initials: R
authors.lastname: Bertram
authors.affiliation: Bacterial and Parasitic Diseases Research Division, National Institute of Animal Health, National Agriculture and Food Research Organization, Tsukuba, Japan; The United Graduate School of Veterinary Sciences, Gifu University, Gifu, Japan.
authors.forename: D
authors.initials: D
authors.lastname: Takamatsu
authors.affiliation: Institute for Microbiology, University of Veterinary Medicine Hannover, Hannover, Germany.
authors.forename: P
authors.initials: P
authors.lastname: Valentin-Weigand
authors.affiliation: Institute for Microbiology, University of Veterinary Medicine Hannover, Hannover, Germany.
authors.forename: R
authors.initials: R
authors.lastname: Goethe
mesh_terms: D023061:Gene Order; D056426:Genetic Loci; D059765:Homologous Recombination; D019426:Integrases / Q000378:metabolism*; D016297:Mutagenesis, Site-Directed / Q000379:methods*; D017011:Streptococcus suis / Q000235:genetics* / Q000378:metabolism*
chemical_list: C045073:Cre recombinase; D019426:Integrases
keywords: Cre–lox system; Markerless mutagenesis; Streptococcus suis