title: Identification of bluetongue virus and epizootic hemorrhagic disease virus serotypes in French Guiana in 2011 and 2012.
abstract: In French Guiana, the sero- and viro-prevalence of Bluetongue virus (BTV) is high but the circulating serotypes remain unknown. No data are available regarding the prevalence of Epizootic hemorrhagic disease (EHD). This study was conducted to assess the prevalence and to identify the circulating serotypes of these two Orbiviruses in this region (BTV and EHDV). Blood samples were collected in main livestock areas, from 122 young cattle between June and August 2011, to perform virological (PCR and viral isolation) and serological (ELISA) analyses. Moreover, samples from sheep and goat showing BTV-like clinical signs and from newly imported animals were analyzed using the same assays. Results confirmed an important viral circulation, with viro- and seroprevalence of 85% and 84% and 60% and 40% for BTV and EHDV, respectively. Ten Orbivirus serotypes were identified (BTV-1, 2, 6, 10, 12, 13, 17 and 24, EHDV-1 and 6). The circulation of many serotypes in intertropical America and in the Caribbean region underlines the need to establish measures to monitor and control animal movements.
authors.affiliation: ANSES/INRA/ENVA-UPEC, UMR 1161 Virologie, 23 avenue du général de Gaulle, 94700 Maisons Alfort, France. Electronic address: cyril.viarouge@anses.fr.
authors.forename: Cyril
authors.initials: C
authors.lastname: Viarouge
authors.affiliation: CIRAD, UMR CMAEE, Campus International de Baillarguet TA A-DIR/B, F34398 Montpellier, France; INRA, UMR 1309, Campus International de Baillarguet TA A-DIR/B, F34398 Montpellier, France. Electronic address: renaud.lancelot@cirad.fr.
authors.forename: Renaud
authors.initials: R
authors.lastname: Lancelot
authors.affiliation: DAAF Guyane Santé et Protection Animales et Végétales, Parc Rebard, B.P. 5002, 97 305 Cayenne Cedex, France. Electronic address: germain.rives@hotmail.fr.
authors.forename: Germain
authors.initials: G
authors.lastname: Rives
authors.affiliation: ANSES/INRA/ENVA-UPEC, UMR 1161 Virologie, 23 avenue du général de Gaulle, 94700 Maisons Alfort, France. Electronic address: emmanuel.breard@anses.fr.
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Bréard
authors.affiliation: DAAF Guyane Santé et Protection Animales et Végétales, Parc Rebard, B.P. 5002, 97 305 Cayenne Cedex, France. Electronic address: manuelle.miller@fvi-atf.org.
authors.forename: Manuelle
authors.initials: M
authors.lastname: Miller
authors.affiliation: DAAF Guyane Santé et Protection Animales et Végétales, Parc Rebard, B.P. 5002, 97 305 Cayenne Cedex, France. Electronic address: xavier.baudrimont@agriculture.gouv.fr.
authors.forename: Xavier
authors.initials: X
authors.lastname: Baudrimont
authors.affiliation: ANSES/INRA/ENVA-UPEC, UMR 1161 Virologie, 23 avenue du général de Gaulle, 94700 Maisons Alfort, France. Electronic address: vdoceul@vet-alfort.fr.
authors.forename: Virginie
authors.initials: V
authors.lastname: Doceul
authors.affiliation: ANSES/INRA/ENVA-UPEC, UMR 1161 Virologie, 23 avenue du général de Gaulle, 94700 Maisons Alfort, France. Electronic address: dvitour@vet-alfort.fr.
authors.forename: Damien
authors.initials: D
authors.lastname: Vitour
authors.affiliation: ANSES/INRA/ENVA-UPEC, UMR 1161 Virologie, 23 avenue du général de Gaulle, 94700 Maisons Alfort, France. Electronic address: szientara@vet-alfort.fr.
authors.forename: Stéphan
authors.initials: S
authors.lastname: Zientara
authors.affiliation: ANSES/INRA/ENVA-UPEC, UMR 1161 Virologie, 23 avenue du général de Gaulle, 94700 Maisons Alfort, France. Electronic address: corinne.sailleau@anses.fr.
authors.forename: Corinne
authors.initials: C
authors.lastname: Sailleau
mesh_terms: D000818:Animals; D001483:Base Sequence; D001819:Bluetongue / Q000453:epidemiology*; D001820:Bluetongue virus / Q000235:genetics*; D002417:Cattle; D017931:DNA Primers / Q000235:genetics; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005616:French Guiana / Q000453:epidemiology; D006041:Goats; D018164:Hemorrhagic Disease Virus, Epizootic / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000662:veterinary; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D017422:Sequence Analysis, DNA / Q000662:veterinary; D016036:Seroepidemiologic Studies; D065288:Serogroup; D034561:Sheep, Domestic; D013045:Species Specificity
chemical_list: D017931:DNA Primers
keywords: Bluetongue; Epizootic hemorrhagic disease; Orbivirus