title: A highly sensitive resonance light scattering probe for Alzheimer's amyloid-β peptide based on Fe3O4@Au composites.
abstract: The fabrication of Fe3O4@Au composites as a novel resonance light scattering (RLS) probe for the sensitive detection of Alzheimer's amyloid-β peptide (Aβ) was demonstrated. Amino groups coated magnetic Fe3O4 nanoparticles were covered with gold shell by the classical Frens method. The resultant colloids were characterized with X-ray diffraction (XRD), transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS), dynamic light scattering (DLS) and UV-visible spectra. The results indicated that the composite particles with core/shell structure and an average diameter of ~ 320 nm were stable and biocompatible. The RLS intensity of Fe3O4@Au composites was significantly enhanced by interacting with Aβ. Under optimal conditions, good linear relationship between the ratio of RLS intensity I/I0 at 463.0 nm and the logarithmic value of Aβ concentration in the range of 5.0 × 10(-15)-5.56 × 10(-9)M was found. The limit of detection (LOD) was 1.2 × 10(-15)M. The proposed method is simple, sensitive and cost-effective and complementary to other existing methods for protein analysis.
authors.affiliation: College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, PR China.
authors.forename: Ling
authors.initials: L
authors.lastname: Yu
authors.affiliation: College of Chemistry and Chemical Engineering, Shangqiu Normal University, Shangqiu 476000, PR China.
authors.forename: Yintang
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: College of Chemistry and Molecular Engineering, Zhengzhou University, Zhengzhou 450052, PR China.
authors.forename: Ran
authors.initials: R
authors.lastname: Chen
authors.affiliation: College of Chemistry and Molecular Engineering, Zhengzhou University, Zhengzhou 450052, PR China.
authors.forename: Danhua
authors.initials: D
authors.lastname: Zhang
authors.affiliation: College of Chemistry and Chemical Engineering, Shangqiu Normal University, Shangqiu 476000, PR China.
authors.forename: Xiuhua
authors.initials: X
authors.lastname: Wei
authors.affiliation: College of Chemistry and Chemical Engineering, Shangqiu Normal University, Shangqiu 476000, PR China.
authors.forename: Fang
authors.initials: F
authors.lastname: Chen
authors.affiliation: College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, PR China.
authors.forename: Jianxiu
authors.initials: J
authors.lastname: Wang
authors.affiliation: College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, PR China; College of Chemistry and Chemical Engineering, Shangqiu Normal University, Shangqiu 476000, PR China. Electronic address: xumaotian@163.com.
authors.forename: Maotian
authors.initials: M
authors.lastname: Xu
mesh_terms: D016229:Amyloid beta-Peptides / Q000032:analysis*; D005290:Ferric Compounds / Q000737:chemistry*; D006046:Gold / Q000737:chemistry*; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry*; D046529:Microscopy, Electron, Transmission; D053761:Nanocomposites / Q000737:chemistry*; D056951:Photoelectron Spectroscopy; D012542:Scattering, Radiation; D014961:X-Ray Diffraction
chemical_list: D016229:Amyloid beta-Peptides; D005290:Ferric Compounds; C000499:ferric oxide; D006046:Gold
keywords: Amyloid-β peptide; Core/shell; Fe(3)O(4)@Au composites; Resonance light scattering