title: [Study of screening nephroprotective bioactive substances based on triple-color fluorescence probes in Carthami flos].
abstract: In this study, an approach based on triple-color fluorescence probes was developed for screening potential nephro-protective bioactive substances. Three fluorescent probes (i. e. FDA, MTR and Hoechst 33342) were used to label HK-2 cells injured by doxorubicin hydrochloride, and cellular fluorescence images were subsequently acquired and analyzed by a cellular-fluorescence image microscopy platform. The established method was applied to screening 53 components of Carthami Flos, and three components C17, C18 and C19 were found to exhibit nephroprotective effects against doxorubicin hydrochloride induced injury on HK-2 cells. Eight compounds (i. e. hydroxysafflor yellow A, 6-hydroxykaempferol-3-O-rutinoside-6-O-glucoside, 6-hydroxykaempferol-3,6-di-O-gluco-side or 6-hydroxykaempferol-6, 7-di-O-glucoside, 6-hydroxykaempferol-3-O-rutinoside, 6-hydroxykaempferol-3-O-glucoside or 6-hydroxykaempferol-7-O-glucoside, rutin, isoquercetin, and kaempferol-3-O-rutinoside) in components C17, C18 and C19 were preliminarily identified by liquid chromatography-mass spectrometry (LC-MS). Isoquercetin, rutin, kaempferol-3-O-rutinoside, and hydroxysafflor yellow A were confirmed by comparing with reference substances, Further study indicated that these four compounds had moderate nephroprotective effects, while isoquercetin showed a significant nephroprotective effect in a dose-dependent manner. These results suggest that isoquercetin, rutin, kaempferol-3-O-rutinoside and hydroxysafflor yellow A might be the nephroprotective bioactive substances in Carthami Flos.
authors.forename: Xiao-Hong
authors.initials: XH
authors.lastname: Lan
authors.forename: Shun
authors.initials: S
authors.lastname: Xiao
authors.forename: Wan
authors.initials: W
authors.lastname: Gong
authors.forename: Yi
authors.initials: Y
authors.lastname: Wang
authors.forename: Xiao-Ping
authors.initials: XP
authors.lastname: Zhao
mesh_terms: D031178:Carthamus / Q000737:chemistry*; D002460:Cell Line; D002851:Chromatography, High Pressure Liquid; D004305:Dose-Response Relationship, Drug; D004353:Drug Evaluation, Preclinical; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000494:pharmacology; D035264:Flowers / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry; D006801:Humans; D007668:Kidney / Q000737:chemistry / Q000166:cytology / Q000187:drug effects; D020011:Protective Agents / Q000737:chemistry* / Q000494:pharmacology
chemical_list: D004365:Drugs, Chinese Herbal; D005456:Fluorescent Dyes; D020011:Protective Agents