title: Polydopamine-based superparamagnetic molecularly imprinted polymer nanospheres for efficient protein recognition.
abstract: A new strategy for synthesis of superparamagnetic molecularly imprinted polymer nanospheres (MIPNSs) for efficient protein recognition is described here. Homogeneous hydroxyl group functionalized Fe3O4/polymethyl methacrylate (PMMA) composite nanospheres were prepared using improved miniemulsion polymerization. Uniform superparamagnetic MIPNSs were obtained via self-polymerization of dopamine (DA) on the surface of Fe3O4/PMMA composite nanospheres in the presence of lysozyme (lyz) template. The as-synthesized Fe3O4/PMMA/PDA MIPNSs had average diameters of 180 nm, high saturation magnetization and a good magnetic response. The lyz-imprinted Fe3O4/PMMA/PDA MIPNSs exhibited specific recognition and efficient adsorption capacity toward lyz template. The amount of lyz adsorbed onto the lyz-imprinted Fe3O4/PMMA/PDA MIPNSs was about 4 times greater than that of the Fe3O4/PMMA/PDA non-imprinted polymer nanospheres (NIPNSs) and about 14, 5, and 5 times greater than that of BSA, BHb, and cyt C, respectively.
authors.affiliation: National Engineering Research Center for Biomaterials, Sichuan University, Chengdu 610064, PR China.
authors.forename: Fang
authors.initials: F
authors.lastname: Lan
authors.affiliation: National Engineering Research Center for Biomaterials, Sichuan University, Chengdu 610064, PR China.
authors.forename: Shaohua
authors.initials: S
authors.lastname: Ma
authors.affiliation: National Engineering Research Center for Biomaterials, Sichuan University, Chengdu 610064, PR China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Yang
authors.affiliation: National Engineering Research Center for Biomaterials, Sichuan University, Chengdu 610064, PR China.
authors.forename: Liqin
authors.initials: L
authors.lastname: Xie
authors.affiliation: National Engineering Research Center for Biomaterials, Sichuan University, Chengdu 610064, PR China. Electronic address: Yaowu_amanda@126.com.
authors.forename: Yao
authors.initials: Y
authors.lastname: Wu
authors.affiliation: National Engineering Research Center for Biomaterials, Sichuan University, Chengdu 610064, PR China. Electronic address: zwgu@scu.edu.cn.
authors.forename: Zhongwei
authors.initials: Z
authors.lastname: Gu
mesh_terms: D007211:Indoles / Q000737:chemistry*; D054118:Nanospheres / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D019904:Polymethyl Methacrylate / Q000737:chemistry; D011506:Proteins / Q000737:chemistry*
chemical_list: D007211:Indoles; D011108:Polymers; D011506:Proteins; C568283:polydopamine; D019904:Polymethyl Methacrylate
keywords: Molecularly imprinted polymer nanospheres (MIPNSs); Polydopamine; Polymethyl methacrylate (PMMA); Protein recognition; Superparamagnetic