title: [Immune diagnostics of disorders in the IFN-alpha/NK-cell system in patients with frequently recurrent herpes simplex].
abstract: OBJECTIVE
  Study of features of NK-cell response to the effect of recombinant IFN-alpha in complex with evaluation of the ability to synthesize inherent IFN-alpha in patients with frequently recurrent herpes simplex (FRHS).
  
  
  METHODS
  48 patients with genital (n = 31), labial (n = 10) and mixed localization (n = 7) FRHS diagnosis were observed. 31 healthy donors composed the control group. MC were cultivated in the presence of a recombinant human IFN-alpha2b at the concentration of 10, 100 and 1000 U/ml for 24 hours. NK-cell response to the effect of IFN-alpha was evaluated after 24 hours using flow cytometry by degranulation reaction and in the NK-activity test. IFN-alpha synthesis was evaluated in HSV-1, HSV-2 and Newcastle disease virus stimulated cell supernatants by EIA method.
  
  
  RESULTS
  Patients with FRHS were established to be a heterogeneous group by parameters in the IFN-alpha/NK-cell cytotoxicity system. 2 types of NK-cell response to the stimulation by recombinant IFN-alpha were identified. Type A is characterized by a decrease of NK-cell response to IFN-alpha in the remission phase and does not have this defect in the exacerbation phase. Synthesis of inherent IFN-alpha in response to viral inductors for type A was comparable with the response in healthy donors in both phases. On the contrary type B having normal sensitivity of NK-cells to IFN-alpha in the remission phase is characterized by a decrease of this parameter in the exacerbation phase for more than 3 times. Synthesis of inherent IFN-alpha in response to viral inductors during type B is increased in the remission phase and decreased in the exacerbation phase.
  
  
  CONCLUSIONS
  During immune-correcting therapy of FRHS a personalized approach taking into account features of NK-cell response to IFN-alpha is necessary, because types A and B have principal differences by cytotoxicity parameters of NK-cells and their change under the effect of IFN-alpha, as well as by parameters of IFN-alpha synthesis in response to viral inductors at various phases of the clinical process.
authors.forename: A V
authors.initials: AV
authors.lastname: Karsonova
authors.forename: A E
authors.initials: AE
authors.lastname: Shulzhenko
authors.forename: A V
authors.initials: AV
authors.lastname: Karaulov
mesh_terms: D000328:Adult; D002908:Chronic Disease / Q000628:therapy; D005260:Female; D006561:Herpes Simplex / Q000235:genetics / Q000276:immunology* / Q000628:therapy* / Q000821:virology; D018259:Herpesvirus 1, Human / Q000187:drug effects / Q000276:immunology; D018258:Herpesvirus 2, Human / Q000187:drug effects / Q000276:immunology; D006801:Humans; D016898:Interferon-alpha / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D007694:Killer Cells, Natural / Q000187:drug effects / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D057285:Precision Medicine; D012008:Recurrence; D018139:Simplexvirus / Q000187:drug effects / Q000276:immunology
chemical_list: D016898:Interferon-alpha