title: Asymmetric reduction of 4-hydroxy-2-butanone to (R)-1,3-butanediol with absolute stereochemical selectivity by a newly isolated strain of Pichia jadinii.
abstract: In this study, a novel strain of Pichia jadinii, HBY61, capable of the biocatalysis of 4-hydroxy-2-butanone (4H2B) to (R)-1,3-BD was isolated. HBY61 produced (R)-1,3-BD with high activity and absolute stereochemical selectivity (100 % e.e). Glucose and beef extract were found to be the key factors governing the fermentation, and their optimal concentrations were determined to be 84.2 and 43.7 g/L, respectively. The optimal bioconversion conditions of 4H2B catalyzed by HBY61 were pH 7.4, 30 °C, and 250 rpm with 6 % (v/v) glucose as the co-substrate. Accordingly, when 45 g/L of 4H2B was divided into three equal parts and added successively into the system at set time intervals, the maximum (R)-1,3-BD concentration reached 38.3 g/L with high yield (85.1 %) and strict 100 % enantioselectivity. Compared with previously reported yields for the biocatalytic production of (R)-1,3-BD, the use of strain HBY61 provided a high yield with excellent stereoselectivity.
authors.affiliation: The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, 1800 Lihu Avenue, Wuxi, Jiangsu, 214122, People's Republic of China.
authors.forename: Taowei
authors.initials: T
authors.lastname: Yang
authors.forename: Zaiwei
authors.initials: Z
authors.lastname: Man
authors.forename: Zhiming
authors.initials: Z
authors.lastname: Rao
authors.forename: Meijuan
authors.initials: M
authors.lastname: Xu
authors.forename: Xian
authors.initials: X
authors.lastname: Zhang
authors.forename: Zhenghong
authors.initials: Z
authors.lastname: Xu
mesh_terms: D055162:Biocatalysis; D002074:Butanones / Q000378:metabolism*; D002072:Butylene Glycols / Q000737:chemistry / Q000378:metabolism*; D005285:Fermentation; D010843:Pichia / Q000145:classification / Q000302:isolation & purification / Q000378:metabolism* / Q000648:ultrastructure; D013237:Stereoisomerism
chemical_list: D002074:Butanones; D002072:Butylene Glycols; C028491:1,3-butylene glycol; C000597994:4-hydroxy-2-butanone