title: Cotton rat (Sigmodon hispidus) signaling lymphocyte activation molecule (CD150) is an entry receptor for measles virus.
abstract: Cotton rats (Sigmodon hispidus) replicate measles virus (MV) after intranasal infection in the respiratory tract and lymphoid tissue. We have cloned the cotton rat signaling lymphocytic activation molecule (CD150, SLAM) in order to investigate its role as a potential receptor for MV. Cotton rat CD150 displays 58% and 78% amino acid homology with human and mouse CD150, respectively. By staining with a newly generated cotton rat CD150 specific monoclonal antibody expression of CD150 was confirmed in cotton rat lymphoid cells and in tissues with a pattern of expression similar to mouse and humans. Previously, binding of MV hemagglutinin has been shown to be dependent on amino acids 60, 61 and 63 in the V region of CD150. The human molecule contains isoleucine, histidine and valine at these positions and binds to MV-H whereas the mouse molecule contains valine, arginine and leucine and does not function as a receptor for MV. In the cotton rat molecule, amino acids 61 and 63 are identical with the mouse molecule and amino acid 60 with the human molecule. After transfection with cotton rat CD150 HEK 293 T cells became susceptible to infection with single cycle VSV pseudotype virus expressing wild type MV glycoproteins and with a MV wildtype virus. After infection, cells expressing cotton rat CD150 replicated virus to lower levels than cells expressing the human molecule and formed smaller plaques. These data might explain why the cotton rat is a semipermissive model for measles virus infection.
authors.affiliation: Department of Veterinary Biosciences and Center for Microbial Interface Biology, The Ohio State University, Columbus, Ohio, United States of America.
authors.forename: Thomas
authors.initials: T
authors.lastname: Carsillo
authors.affiliation: Department of Veterinary Biosciences and Center for Microbial Interface Biology, The Ohio State University, Columbus, Ohio, United States of America.
authors.forename: Devra
authors.initials: D
authors.lastname: Huey
authors.affiliation: Department of Veterinary Biosciences and Center for Microbial Interface Biology, The Ohio State University, Columbus, Ohio, United States of America.
authors.forename: Amy
authors.initials: A
authors.lastname: Levinsky
authors.affiliation: Institute of Virology and Immunobiology, University of Würzburg, Würzburg, Germany.
authors.forename: Karola
authors.initials: K
authors.lastname: Obojes
authors.affiliation: Institute of Virology and Immunobiology, University of Würzburg, Würzburg, Germany.
authors.forename: Jürgen
authors.initials: J
authors.lastname: Schneider-Schaulies
authors.affiliation: Department of Veterinary Biosciences and Center for Microbial Interface Biology, The Ohio State University, Columbus, Ohio, United States of America.
authors.forename: Stefan
authors.initials: S
authors.lastname: Niewiesk
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D015703:Antigens, CD / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D002522:Chlorocebus aethiops; D003001:Cloning, Molecular; D057809:HEK293 Cells; D006801:Humans; D008459:Measles virus / Q000378:metabolism* / Q000502:physiology; D051379:Mice; D008969:Molecular Sequence Data; D011956:Receptors, Cell Surface / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D016205:Sigmodontinae; D000071177:Signaling Lymphocytic Activation Molecule Family Member 1; D014162:Transfection; D014709:Vero Cells; D014779:Virus Replication
chemical_list: D015703:Antigens, CD; D011956:Receptors, Cell Surface; C000604059:SLAMF1 protein, human; D000071177:Signaling Lymphocytic Activation Molecule Family Member 1