title: Electrochemical biotin detection based on magnetic beads and a new magnetic flow cell for screen printed electrode.
abstract: The use of the first flow-cell for magnetic assays with an integrated magnet is reported here. The flow injection analysis system (FIA) is used for biotin determination. The reaction scheme is based on a one step competitive assay between free biotin and biotin labeled with horseradish peroxidase (B-HRP). The mixture of magnetic beads modified with streptavidin (Strep-MB), biotin and B-HRP is left 15 min under stirring and then a washing step is performed. After that, 100 μL of the mixture is injected and after 30s 100 μL of 3,3',5,5'-Tetramethylbenzidine (TMB) is injected and the FIAgram is recorded applying a potential of -0.2V. The linear range obtained is from 0.01 to 1 nM of biotin and the sensitivity is 758 nA/nM. The modification and cleaning of the electrode are performed in an easy way due to the internal magnet of the flow cell.
authors.affiliation: Department of Physical and Analytical Chemistry, Faculty of Chemistry, University of Oviedo, 33006 Oviedo, Spain.
authors.forename: Julien
authors.initials: J
authors.lastname: Biscay
authors.affiliation: Department of Physical and Analytical Chemistry, Faculty of Chemistry, University of Oviedo, 33006 Oviedo, Spain.
authors.forename: María Begoña
authors.initials: MB
authors.lastname: González García
authors.affiliation: Department of Physical and Analytical Chemistry, Faculty of Chemistry, University of Oviedo, 33006 Oviedo, Spain. Electronic address: costa@uniovi.es.
authors.forename: Agustín
authors.initials: A
authors.lastname: Costa García
mesh_terms: D001560:Benzidines / Q000737:chemistry; D015374:Biosensing Techniques / Q000379:methods*; D001710:Biotin / Q000032:analysis*; D004563:Electrochemistry / Q000379:methods*; D004566:Electrodes; D017022:Flow Injection Analysis / Q000379:methods*; D006735:Horseradish Peroxidase / Q000378:metabolism; D018189:Immunomagnetic Separation; D008280:Magnetics
chemical_list: D001560:Benzidines; C021758:3,3',5,5'-tetramethylbenzidine; D001710:Biotin; D006735:Horseradish Peroxidase
keywords: Biotin detection; Flow cell with an integrated magnet; Flow injection analysis; Magnetic beads; Screen printed electrode