title: Unravelling the mechanism of non-ribosomal peptide synthesis by cyclodipeptide synthases.
abstract: Cyclodipeptide synthases form cyclodipeptides from two aminoacyl transfer RNAs. They use a ping-pong mechanism that begins with transfer of the aminoacyl moiety of the first aminoacyl tRNA onto a conserved serine, yielding an aminoacyl enzyme. Combining X-ray crystallography, site-directed mutagenesis and affinity labelling of the cyclodipeptide synthase AlbC, we demonstrate that the covalent intermediate reacts with the aminoacyl moiety of the second aminoacyl tRNA, forming a dipeptidyl enzyme, and identify the aminoacyl-binding sites of the aminoacyl tRNAs.
authors.affiliation: Service d'Ingénierie Moléculaire des Protéines, iBiTec-S, Commissariat à l'Energie Atomique et aux Energies Alternatives (CEA), 91191 Gif-sur-Yvette, France.
authors.forename: Mireille
authors.initials: M
authors.lastname: Moutiez
authors.affiliation: Laboratoire de Biochimie, Unité mixte de Recherche 7654, Ecole Polytechnique, Centre National de la Recherche Scientifique, 91120 Palaiseau, France.
authors.forename: Emmanuelle
authors.initials: E
authors.lastname: Schmitt
authors.affiliation: Service d'Ingénierie Moléculaire des Protéines, iBiTec-S, Commissariat à l'Energie Atomique et aux Energies Alternatives (CEA), 91191 Gif-sur-Yvette, France.
authors.forename: Jérôme
authors.initials: J
authors.lastname: Seguin
authors.affiliation: Service d'Ingénierie Moléculaire des Protéines, iBiTec-S, Commissariat à l'Energie Atomique et aux Energies Alternatives (CEA), 91191 Gif-sur-Yvette, France.
authors.forename: Robert
authors.initials: R
authors.lastname: Thai
authors.affiliation: Service d'Ingénierie Moléculaire des Protéines, iBiTec-S, Commissariat à l'Energie Atomique et aux Energies Alternatives (CEA), 91191 Gif-sur-Yvette, France.
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Favry
authors.affiliation: Service d'Ingénierie Moléculaire des Protéines, iBiTec-S, Commissariat à l'Energie Atomique et aux Energies Alternatives (CEA), 91191 Gif-sur-Yvette, France.
authors.forename: Pascal
authors.initials: P
authors.lastname: Belin
authors.affiliation: Laboratoire de Biochimie, Unité mixte de Recherche 7654, Ecole Polytechnique, Centre National de la Recherche Scientifique, 91120 Palaiseau, France.
authors.forename: Yves
authors.initials: Y
authors.lastname: Mechulam
authors.affiliation: Service d'Ingénierie Moléculaire des Protéines, iBiTec-S, Commissariat à l'Energie Atomique et aux Energies Alternatives (CEA), 91191 Gif-sur-Yvette, France.
authors.forename: Muriel
authors.initials: M
authors.lastname: Gondry
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry*; D002384:Catalysis; D020134:Catalytic Domain; D018360:Crystallography, X-Ray; D008968:Molecular Conformation; D016297:Mutagenesis, Site-Directed; D010452:Peptide Biosynthesis; D010453:Peptide Synthases / Q000737:chemistry*; D012343:RNA, Transfer / Q000737:chemistry; D012270:Ribosomes / Q000737:chemistry*; D012694:Serine / Q000737:chemistry; D013302:Streptomyces / Q000737:chemistry
chemical_list: D001426:Bacterial Proteins; D012694:Serine; D012343:RNA, Transfer; D010453:Peptide Synthases