title: Influence of manufacturing processes on cell surface properties of probiotic strain Lactobacillus rhamnosus Lcr35®.
abstract: The influence of the industrial process on the properties of probiotics, administered as complex manufactured products, has been poorly investigated. In the present study, we comparatively assessed the cell wall characteristics of the probiotic strain Lactobacillus rhamnosus Lcr35® together with three of its commercial formulations with intestinal applications. Putative secreted and transmembrane-protein-encoding genes were initially searched in silico in the genome of L. rhamnosus Lcr35®. A total of 369 candidate genes were identified which expressions were followed using a custom Lactobacillus DNA chip. Among them, 60 or 67 genes had their expression either upregulated or downregulated in the Lcr Restituo® packet or capsule formulations, compared to the native Lcr35® strain. Moreover, our data showed that the probiotic formulations (Lcr Lenio®, Lcr restituo® capsule and packet) showed a better capacity to adhere to intestinal epithelial Caco-2 cells than the native Lcr35® strain. Microbial (MATS) tests showed that the probiotic was an electron donor and that they were more hydrophilic than the native strain. The enhanced adhesion capacity of the active pharmaceutical ingredients (APIs) to epithelial Caco-2 cells and their antipathogen effect could be due to this greater surface hydrophilic character. These findings suggest that the manufacturing process influences the protein composition and the chemical properties of the cell wall. It is therefore likely that the antipathogen effect of the formulation is modulated by the industrial process. Screening of the manufactured products' properties would therefore represent an essential step in evaluating the effects of probiotic strains.
authors.affiliation: Département Recherche et Développement-Probionov, Rue des frères Lumières, 15130, Arpajon-sur-Cère, France, a.nivoliez@probionov.com.
authors.forename: Adrien
authors.initials: A
authors.lastname: Nivoliez
authors.forename: Philippe
authors.initials: P
authors.lastname: Veisseire
authors.forename: Elina
authors.initials: E
authors.lastname: Alaterre
authors.forename: Caroline
authors.initials: C
authors.lastname: Dausset
authors.forename: Fabrice
authors.initials: F
authors.lastname: Baptiste
authors.forename: Olivier
authors.initials: O
authors.lastname: Camarès
authors.forename: Marylise
authors.initials: M
authors.lastname: Paquet-Gachinat
authors.forename: Muriel
authors.initials: M
authors.lastname: Bonnet
authors.forename: Christiane
authors.initials: C
authors.lastname: Forestier
authors.forename: Stéphanie
authors.initials: S
authors.lastname: Bornes
mesh_terms: D001422:Bacterial Adhesion; D018938:Caco-2 Cells; D002473:Cell Wall / Q000737:chemistry* / Q000235:genetics; D002626:Chemistry, Pharmaceutical; D004847:Epithelial Cells / Q000382:microbiology; D020869:Gene Expression Profiling; D006801:Humans; D052201:Lacticaseibacillus rhamnosus / Q000737:chemistry* / Q000235:genetics / Q000502:physiology; D008565:Membrane Proteins / Q000032:analysis* / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis; D019936:Probiotics / Q000737:chemistry*; D013499:Surface Properties; D013678:Technology, Pharmaceutical / Q000379:methods
chemical_list: D008565:Membrane Proteins