title: Sugar regulation of SUGAR TRANSPORTER PROTEIN 1 (STP1) expression in Arabidopsis thaliana.
abstract: Sugars regulate the expression of many genes at the transcriptional level. In Arabidopsis thaliana, sugars induce or repress the expression of >1800 genes, including the STP1 (SUGAR TRANSPORTER PROTEIN 1) gene, which encodes an H(+)/monosaccharide cotransporter. STP1 transcript levels decrease more rapidly after the addition of low concentrations of sugars than the levels of other repressed genes, such as DIN6 (DARK-INDUCED 6). We found that this regulation is exerted at the transcriptional level and is initiated by phosphorylatable sugars. Interestingly, the sugar signal that modulates STP1 expression is transmitted through a HEXOKINASE 1-independent signalling pathway. Finally, analysis of the STP1 5' regulatory region allowed us to delimit a region of 309bp that contains the cis elements implicated in the glucose regulation of STP1 expression. Putative cis-acting elements involved in this response were identified.
authors.affiliation: Instituto de Biotecnología, Universidad Nacional Autónoma de México, Av. Universidad 2001, Col. Chamilpa, Cuernavaca, Morelos. México. C.P. 62210, Mexico eliza@ibt.unam.mx patricia@ibt.unam.mx.
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Cordoba
authors.affiliation: Instituto de Biotecnología, Universidad Nacional Autónoma de México, Av. Universidad 2001, Col. Chamilpa, Cuernavaca, Morelos. México. C.P. 62210, Mexico.
authors.forename: Denise Lizeth
authors.initials: DL
authors.lastname: Aceves-Zamudio
authors.affiliation: Instituto de Biotecnología, Universidad Nacional Autónoma de México, Av. Universidad 2001, Col. Chamilpa, Cuernavaca, Morelos. México. C.P. 62210, Mexico.
authors.forename: Alma Fabiola
authors.initials: AF
authors.lastname: Hernández-Bernal
authors.affiliation: Instituto de Biotecnología, Universidad Nacional Autónoma de México, Av. Universidad 2001, Col. Chamilpa, Cuernavaca, Morelos. México. C.P. 62210, Mexico.
authors.forename: Maricela
authors.initials: M
authors.lastname: Ramos-Vega
authors.affiliation: Instituto de Biotecnología, Universidad Nacional Autónoma de México, Av. Universidad 2001, Col. Chamilpa, Cuernavaca, Morelos. México. C.P. 62210, Mexico eliza@ibt.unam.mx patricia@ibt.unam.mx.
authors.forename: Patricia
authors.initials: P
authors.lastname: León
mesh_terms: D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D050260:Carbohydrate Metabolism; D018506:Gene Expression Regulation, Plant; D009004:Monosaccharide Transport Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D015398:Signal Transduction
chemical_list: D029681:Arabidopsis Proteins; D009004:Monosaccharide Transport Proteins; C000605031:STP1 protein, Arabidopsis
keywords: Arabidopsis; STP1; sugar repression; sugar signalling; sugar transporter; transcriptional regulation.