title: Reduction of methanol in brewed wine by the use of atmospheric and room-temperature plasma method and the combination optimization of malt with different adjuncts.
abstract: Methanol, often generated in brewed wine, is highly toxic for human health. To decrease the methanol content of the brewed wine, atmospheric and room-temperature plasma (ARTP) was used as a new mutagenesis tool to generate a mutant of Saccharomyces cerevisiae with lower methanol content. Headspace gas chromatography was used to determine the identity and concentration of methanol with butyl acetate as internal standard in brewed wine. With 47.4% higher and 26.3% positive mutation rates were obtained, the ARTP jet exhibited a strong effect on mutation breeding of S. cerevisiae. The mutant S. cerevisiae S12 exhibited the lowest methanol content, which was decreased by 72.54% compared with that of the wild-type strain. Subsequently, the mutant S. cerevisiae S12 was used to ferment different combinations of malt and adjuncts for lower methanol content and higher alcoholic content. It was shown that the culture 6#, which was 60% malt, 20% wheat, and 20% corn, was the best combinations of malt and adjuncts, with the lowest methanol content (104.8 mg/L), and a relatively higher alcoholic content (15.3%, v/v). The optimal malt-adjunct culture 6#, treated with the glucoamylase dose of 0.04 U/mg of grain released the highest reducing sugars (201.6 mg/mL). It was indicated that the variation in reducing sugars among the combinations of malt and different adjuncts could be due to the dose of exogenous enzymes.
authors.affiliation: School of Biological Science & Engineering, South China Univ. of Technology, Guangzhou, 510006, China; College of Food Science and Engineering, South China Univ. of Technology, Guangzhou, 510640, China.
authors.forename: Ming-Hua
authors.initials: MH
authors.lastname: Liang
authors.forename: Ying-Jie
authors.initials: YJ
authors.lastname: Liang
authors.forename: Jiang-Yan
authors.initials: JY
authors.lastname: Chai
authors.forename: Shi-Shui
authors.initials: SS
authors.lastname: Zhou
authors.forename: Jian-Guo
authors.initials: JG
authors.lastname: Jiang
mesh_terms: D002849:Chromatography, Gas; D003470:Culture Media / Q000737:chemistry; D005285:Fermentation; D000432:Methanol / Q000032:analysis*; D016296:Mutagenesis; D009154:Mutation; D058626:Plasma Gases / Q000737:chemistry; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D013696:Temperature; D014908:Triticum / Q000737:chemistry; D014920:Wine / Q000032:analysis*; D003313:Zea mays / Q000737:chemistry
chemical_list: D003470:Culture Media; D058626:Plasma Gases; D000432:Methanol
keywords: adjunct; atmospheric and room-temperature plasma; brewed wine; headspace gas chromatography; methanol