title: Timing of peripheral blood stem cell yield: comparison of alternative methods with the classic method for CD34+ cell determination.
abstract: Hematopoietic stem cells (HSCs), still represent a certain mystery in biology, have a unique property of dividing into equal cells and repopulating the hematopoietic tissue. This potential enables their use in transplantation treatments. The quality of the HSC grafts for transplantation is evaluated by flow cytometric determination of the CD34(+) cells, which enables optimal timing of the first apheresis and the acquisition of maximal yield of the peripheral blood stem cells (PBSCs). To identify a more efficient method for evaluating CD34(+) cells, we compared the following alternative methods with the reference method: hematopoietic progenitor cells (HPC) enumeration (using the Sysmex XE-2100 analyser), detection of CD133(+) cells, and quantification of aldehyde dehydrogenase activity in the PBSCs. 266 aphereses (84 patients) were evaluated. In the preapheretic blood, the new methods produced data that were in agreement with the reference method. The ROC curves have shown that for the first-day apheresis target, the optimal predictive cut-off value was 0.032 cells/mL for the HPC method (sensitivity 73.4%, specificity 69.3%). HPC method exhibited a definite practical superiority as compared to other methods tested. HPC enumeration could serve as a supplementary method for the optimal timing of the first apheresis; it is simple, rapid, and cheap.
authors.affiliation: 4th Department of Internal Medicine-Hematology, Charles University, Medical Faculty and Teaching Hospital, Sokolska Street 581, 500 05 Hradec Kralove, Czech Republic.
authors.forename: I
authors.initials: I
authors.lastname: Fatorova
authors.affiliation: 4th Department of Internal Medicine-Hematology, Charles University, Medical Faculty and Teaching Hospital, Sokolska Street 581, 500 05 Hradec Kralove, Czech Republic.
authors.forename: M
authors.initials: M
authors.lastname: Blaha
authors.affiliation: 4th Department of Internal Medicine-Hematology, Charles University, Medical Faculty and Teaching Hospital, Sokolska Street 581, 500 05 Hradec Kralove, Czech Republic.
authors.forename: M
authors.initials: M
authors.lastname: Lanska
authors.affiliation: Institute of Immunology and Allergology, Charles University, Medical Faculty and Teaching Hospital, Sokolska Street 581, 500 05 Hradec Kralove, Czech Republic.
authors.forename: D
authors.initials: D
authors.lastname: Vokurkova
authors.affiliation: Institute of Immunology and Allergology, Charles University, Medical Faculty and Teaching Hospital, Sokolska Street 581, 500 05 Hradec Kralove, Czech Republic.
authors.forename: V
authors.initials: V
authors.lastname: Rezacova
authors.affiliation: 4th Department of Internal Medicine-Hematology, Charles University, Medical Faculty and Teaching Hospital, Sokolska Street 581, 500 05 Hradec Kralove, Czech Republic.
authors.forename: P
authors.initials: P
authors.lastname: Zak
mesh_terms: D000071916:AC133 Antigen; D000328:Adult; D000368:Aged; D000444:Aldehyde Dehydrogenase / Q000378:metabolism; D015703:Antigens, CD / Q000378:metabolism; D018952:Antigens, CD34 / Q000378:metabolism*; D005260:Female; D005434:Flow Cytometry / Q000379:methods*; D006023:Glycoproteins / Q000378:metabolism; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000378:metabolism; D006801:Humans; D007937:Leukapheresis; D008297:Male; D008875:Middle Aged; D010455:Peptides / Q000378:metabolism; D012372:ROC Curve; D015203:Reproducibility of Results; D018709:Statistics, Nonparametric; D013997:Time Factors; D055815:Young Adult
chemical_list: D000071916:AC133 Antigen; D015703:Antigens, CD; D018952:Antigens, CD34; D006023:Glycoproteins; C000606023:PROM1 protein, human; D010455:Peptides; D000444:Aldehyde Dehydrogenase