title: Induction of the viable but nonculturable state of Ralstonia solanacearum by low temperature in the soil microcosm and its resuscitation by catalase.
abstract: Ralstonia solanacearum is the causal agent of bacterial wilt on a wide variety of plants, and enters a viable but nonculturable (VBNC) state under stress conditions in soil and water. Here, we adopted an artificial soil microcosm (ASM) to investigate the VBNC state of R. solanacearum induced by low temperature. The culturability of R. solanacearum strains SL341 and GMI1000 rapidly decreased at 4°C in modified ASM (mASM), while it was stably maintained at 25°C in mASM. We hypothesized that bacterial cells at 4°C in mASM are viable but nonculturable. Total protein profiles of SL341 cells at 4°C in mASM did not differ from those of SL341 culturable cells at 25°C in mASM. Moreover, the VBNC cells maintained in the mASM retained respiration activity. Catalase treatment effectively restored the culturability of nonculturable cells in mASM, while temperature increase or other treatments used for resuscitation of other bacteria were not effective. The resuscitated R. solanacearum from VBNC state displayed normal level of bacterial virulence on tomato plants compared with its original culturable bacteria. Expression of omp, oxyR, rpoS, dps, and the 16S rRNA gene quantified by RT-qPCR did not differ significantly between the culturable and VBNC states of R. solanacearum. Our results suggested that the VBNC bacterial cells in mASM induced by low temperature exist in a physiologically unique state.
authors.affiliation: Department of Applied Bioscience, Dong-A University, Busan, Republic of Korea.
authors.forename: Hyun Gi
authors.initials: HG
authors.lastname: Kong
authors.affiliation: Department of Applied Bioscience, Dong-A University, Busan, Republic of Korea.
authors.forename: Ju Young
authors.initials: JY
authors.lastname: Bae
authors.affiliation: Department of Applied Bioscience, Dong-A University, Busan, Republic of Korea.
authors.forename: Hyoung Ju
authors.initials: HJ
authors.lastname: Lee
authors.affiliation: Department of Applied Bioscience, Dong-A University, Busan, Republic of Korea.
authors.forename: Hae Jin
authors.initials: HJ
authors.lastname: Joo
authors.affiliation: Department of Applied Bioscience, Dong-A University, Busan, Republic of Korea.
authors.forename: Eun Joo
authors.initials: EJ
authors.lastname: Jung
authors.affiliation: Department of Applied Bioscience, Dong-A University, Busan, Republic of Korea.
authors.forename: Eunsook
authors.initials: E
authors.lastname: Chung
authors.affiliation: Department of Applied Bioscience, Dong-A University, Busan, Republic of Korea.
authors.forename: Seon-Woo
authors.initials: SW
authors.lastname: Lee
mesh_terms: D002374:Catalase / Q000378:metabolism*; D046508:Culture Techniques; D015964:Gene Expression Regulation, Bacterial; D050296:Microbial Viability; D043368:Ralstonia solanacearum / Q000235:genetics / Q000254:growth & development / Q000502:physiology*; D012987:Soil; D013312:Stress, Physiological; D013696:Temperature
chemical_list: D012987:Soil; D002374:Catalase