title: Switch of PKA substrates from Cubitus interruptus to Smoothened in the Hedgehog signalosome complex.
abstract: Hedgehog (Hh) signalling is crucial for developmental patterning and tissue homeostasis. In Drosophila, Hh signalling is mediated by a bifunctional transcriptional mediator, called Cubitus interruptus (Ci). Protein Kinase A (PKA)-dependent phosphorylation of the serpentine protein Smoothened (Smo) leads to Ci activation, whereas PKA-dependent phosphorylation of Ci leads to the formation of Ci repressor form. The mechanism that switches PKA from an activator to a repressor is not known. Here we show that Hh signalling activation causes PKA to switch its substrates from Ci to Smo within the Hh signalling complex (HSC). In particular, Hh signalling increases the level of Smo, which then outcompetes Ci for association with PKA and causes a switch in PKA substrate recognition. We propose a new model in which the PKA is constitutively present and active within the HSC, and in which the relative levels of Ci and Smo within the HSC determine differential activation and cellular response to Hh signalling.
authors.affiliation: Department of Genetics, University of Cambridge, Downing Street, Cambridge CB2 3EH, UK.
authors.forename: Nadia
authors.initials: N
authors.lastname: Ranieri
authors.affiliation: 1] University Nice Sophia Antipolis, iBV, UMR 7277, 06100 Nice, France [2] CNRS, iBV, UMR 7277, Parc Valrose, 06100 Nice, France [3] INSERM, iBV, U10891, 06100 Nice, France.
authors.forename: Pascal P
authors.initials: PP
authors.lastname: Thérond
authors.affiliation: 1] University Nice Sophia Antipolis, iBV, UMR 7277, 06100 Nice, France [2] CNRS, iBV, UMR 7277, Parc Valrose, 06100 Nice, France [3] INSERM, iBV, U10891, 06100 Nice, France.
authors.forename: Laurent
authors.initials: L
authors.lastname: Ruel
mesh_terms: D000818:Animals; D015153:Blotting, Western; D017868:Cyclic AMP-Dependent Protein Kinases / Q000378:metabolism*; D018076:DNA, Complementary / Q000235:genetics; D004268:DNA-Binding Proteins / Q000378:metabolism*; D004330:Drosophila / Q000378:metabolism*; D029721:Drosophila Proteins / Q000378:metabolism*; D053823:Hedgehog Proteins / Q000378:metabolism*; D047468:Immunoprecipitation; D008954:Models, Biological; D010766:Phosphorylation; D034622:RNA Interference; D043562:Receptors, G-Protein-Coupled / Q000378:metabolism*; D015398:Signal Transduction / Q000502:physiology*; D000072086:Smoothened Receptor; D013379:Substrate Specificity; D014157:Transcription Factors / Q000378:metabolism*
chemical_list: D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; D053823:Hedgehog Proteins; D043562:Receptors, G-Protein-Coupled; D000072086:Smoothened Receptor; D014157:Transcription Factors; C092937:ci protein, Drosophila; C100664:smo protein, Drosophila; C077245:hh protein, Drosophila; D017868:Cyclic AMP-Dependent Protein Kinases