title: Step by step manipulation of the CryoCapsule with HPM high pressure freezers.
abstract: The CryoCapsule is a tool dedicated to correlative light to electron microscopy experiments. Focused on simplifying the specimen manipulation throughout the entire workflow from live-cell imaging to freeze substitution following cryofixation by high pressure freezing, we introduce here a step by step procedure to use the CryoCapsule either with the high pressure freezing machines: HPM010 or the HPM100.
authors.affiliation: Centre de Recherche, Institut Curie, Paris, France; Structure and Membrane Compartments, CNRS UMR144, Paris, France.
authors.forename: Xavier
authors.initials: X
authors.lastname: Heiligenstein
authors.affiliation: Centre de Recherche, Institut Curie, Paris, France; Structure and Membrane Compartments, CNRS UMR144, Paris, France; Cell and Tissue Imaging Facility (PICT-IBiSA), CNRS UMR144, Paris, France.
authors.forename: Ilse
authors.initials: I
authors.lastname: Hurbain
authors.affiliation: Centre de Recherche, Institut Curie, Paris, France; Structure and Membrane Compartments, CNRS UMR144, Paris, France.
authors.forename: Cédric
authors.initials: C
authors.lastname: Delevoye
authors.affiliation: Centre de Recherche, Institut Curie, Paris, France; Cell and Tissue Imaging Facility (PICT-IBiSA), CNRS UMR144, Paris, France; Spatio-Temporal Modeling Imaging and Cellular Dynamics, CNRS UMR144, Paris, France.
authors.forename: Jean
authors.initials: J
authors.lastname: Salamero
authors.affiliation: Department of Structural Biology and Genomics, Institut de Génétique et de Biologie Moléculaire et Cellulaire, INSERM, U964, CNRS UMR7104, Illkirch, France.
authors.forename: Claude
authors.initials: C
authors.lastname: Antony
authors.affiliation: Centre de Recherche, Institut Curie, Paris, France; Structure and Membrane Compartments, CNRS UMR144, Paris, France; Cell and Tissue Imaging Facility (PICT-IBiSA), CNRS UMR144, Paris, France.
authors.forename: Graca
authors.initials: G
authors.lastname: Raposo
mesh_terms: D015703:Antigens, CD / Q000096:biosynthesis; D001426:Bacterial Proteins / Q000096:biosynthesis; D045744:Cell Line, Tumor; D015925:Cryopreservation; D006801:Humans; D037181:Lectins, C-Type / Q000096:biosynthesis; D008164:Luminescent Proteins / Q000096:biosynthesis; D037241:Mannose-Binding Lectins / Q000096:biosynthesis; D046529:Microscopy, Electron, Transmission / Q000295:instrumentation / Q000379:methods; D008856:Microscopy, Fluorescence / Q000295:instrumentation / Q000379:methods; D011312:Pressure; D011993:Recombinant Fusion Proteins
chemical_list: D015703:Antigens, CD; D001426:Bacterial Proteins; C121916:CD207 protein, human; D037181:Lectins, C-Type; D008164:Luminescent Proteins; D037241:Mannose-Binding Lectins; D011993:Recombinant Fusion Proteins; C054562:yellow fluorescent protein, Bacteria
keywords: Correlative light and electron microscopy; CryoCapsule; HPM010; HPM100; High pressure freezing; Live-cell imaging