title: Backbone (1)H, (13)C, and (15)N resonance assignments of the Fc fragment of human immunoglobulin G glycoprotein.
abstract: The Fc portion of immunoglobulin G (IgG) recruits complements and its cognate receptors, thereby promoting defensive mechanisms in the humoral immune system. These effector functions critically depend on N-glycosylation at the Fc region, which is therefore regarded as a crucial factor in the design and production of therapeutic antibodies. NMR spectroscopy plays a unique role in the characterization of conformational dynamics and intermolecular interactions of IgG-Fc in solutions. Here, we report NMR assignments of the glycosylated Fc fragment (Mr 53 kDa), cleaved from a chimeric antibody with human IgG1 constant regions, which was produced in Chinese hamster ovary cells with uniform (13)C- and (15)N-labeling.
authors.affiliation: Graduate School and Faculty of Pharmaceutical Sciences, Nagoya City University, Nagoya, 467-8603, Japan.
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Yagi
authors.affiliation: Graduate School and Faculty of Pharmaceutical Sciences, Nagoya City University, Nagoya, 467-8603, Japan.|Okazaki Institute for Integrative Bioscience and Institute for Molecular Science, National Institutes of Natural Sciences, Okazaki, Aichi, 444-8787, Japan.
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Graduate School and Faculty of Pharmaceutical Sciences, Nagoya City University, Nagoya, 467-8603, Japan.|Okazaki Institute for Integrative Bioscience and Institute for Molecular Science, National Institutes of Natural Sciences, Okazaki, Aichi, 444-8787, Japan.
authors.forename: Maho
authors.initials: M
authors.lastname: Yagi-Utsumi
authors.affiliation: Graduate School and Faculty of Pharmaceutical Sciences, Nagoya City University, Nagoya, 467-8603, Japan.|Okazaki Institute for Integrative Bioscience and Institute for Molecular Science, National Institutes of Natural Sciences, Okazaki, Aichi, 444-8787, Japan.
authors.forename: Takumi
authors.initials: T
authors.lastname: Yamaguchi
authors.affiliation: Kyowa Hakko Kirin, Co., Ltd., Chiyoda-ku, Tokyo, 100-0004, Japan.
authors.forename: Shigeru
authors.initials: S
authors.lastname: Iida
authors.affiliation: Graduate School and Faculty of Pharmaceutical Sciences, Nagoya City University, Nagoya, 467-8603, Japan.|Structural Glycobiology Team, Systems Glycobiology Research Group, RIKEN-Max Planck Joint Research Center, RIKEN Global Research Cluster, 2-1 Hirosawa, Wako, Saitama, 351-0198, Japan.
authors.forename: Yoshiki
authors.initials: Y
authors.lastname: Yamaguchi
authors.affiliation: Graduate School and Faculty of Pharmaceutical Sciences, Nagoya City University, Nagoya, 467-8603, Japan. kkato@phar.nagoya-cu.ac.jp.|Okazaki Institute for Integrative Bioscience and Institute for Molecular Science, National Institutes of Natural Sciences, Okazaki, Aichi, 444-8787, Japan. kkato@phar.nagoya-cu.ac.jp.
authors.forename: Koichi
authors.initials: K
authors.lastname: Kato
mesh_terms: D000818:Animals; D016466:CHO Cells; D066241:Carbon-13 Magnetic Resonance Spectroscopy; D006224:Cricetinae; D003412:Cricetulus; D006023:Glycoproteins / Q000737:chemistry*; D006801:Humans; D007074:Immunoglobulin G / Q000737:chemistry*; D009587:Nitrogen Isotopes; D066244:Proton Magnetic Resonance Spectroscopy; D011961:Receptors, Fc / Q000737:chemistry*
chemical_list: D006023:Glycoproteins; D007074:Immunoglobulin G; D009587:Nitrogen Isotopes; D011961:Receptors, Fc
keywords: Fc; Glycoprotein; Immunoglobulin G; Mammalian expression system; NMR spectroscopy; Resonance assignment