title: Pathological findings in equine herpesvirus 9-induced abortion in rats.
abstract: Pregnant rats were infected experimentally with equine herpesvirus (EHV)-9, a new neurotropic equine herpesvirus serologically similar to EHV-1, during the first and third trimesters. The inoculated dams had mild to severe neurological signs and gave birth to dead fetuses or undersized pups. Rats inoculated during the first and last trimesters had varying degrees of encephalitis as well as abnormalities of the placentas in the form of marked dilation of maternal blood sinusoids and varying degrees of atrophy and necrosis of the trophoblast cells of the labyrinth, the spongiotrophoblasts and the giant cell layer. Virus antigen was detected by immunohistochemistry in the brain and the trophoblast cells of labyrinth, the spongiotrophoblasts and giant cell layer of the placenta in rats inoculated during the first trimester. Virus antigen was detected in fetuses from rats inoculated in the first and last trimesters. Virus DNA was amplified by polymerase chain reaction from the placenta and fetuses of inoculated rats. EHV-9 may induce fetal death and abortion in pregnant dams, possibly caused by direct EHV-9 infection of the placenta and/or fetus as well as the secondary effect of vascular injury.
authors.affiliation: Department of Veterinary Pathology, Faculty of Veterinary Medicine, Kafr El-Sheikh University, Kafr El-Sheikh, Egypt.
authors.forename: N
authors.initials: N
authors.lastname: El-Habashi
authors.affiliation: Department of Veterinary Pathology, Faculty of Veterinary Medicine, Beni-Suef University, Egypt.
authors.forename: E
authors.initials: E
authors.lastname: El-Nahass
authors.affiliation: Department of Pathogenetic Veterinary Sciences, United Graduate School of Veterinary Sciences, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan; Department of Pathology and Clinical Pathology, Faculty of Veterinary Medicine, South Valley University, Qena 83523, Egypt.
authors.forename: M
authors.initials: M
authors.lastname: Haridy
authors.affiliation: Department of Animal Medicine and Infectious Diseases, Faculty of Veterinary Medicine, Sadat City University, Sadat City 32897, Egypt.
authors.forename: M
authors.initials: M
authors.lastname: Nayel
authors.affiliation: Department of Poultry and Rabbit Medicine, Faculty of Veterinary Medicine, Suez Canal University, Egypt.
authors.forename: A A
authors.initials: AA
authors.lastname: Abdelaziz
authors.affiliation: Department of Pathogenetic Veterinary Sciences, United Graduate School of Veterinary Sciences, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Fukushi
authors.affiliation: Department of Pathogenetic Veterinary Sciences, United Graduate School of Veterinary Sciences, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Kuroda
authors.affiliation: Department of Pathogenetic Veterinary Sciences, United Graduate School of Veterinary Sciences, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Sakai
authors.affiliation: Department of Pathogenetic Veterinary Sciences, United Graduate School of Veterinary Sciences, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan. Electronic address: yanai@gifu-u.ac.jp.
authors.forename: T
authors.initials: T
authors.lastname: Yanai
mesh_terms: D000034:Abortion, Veterinary / Q000821:virology*; D000818:Animals; D004195:Disease Models, Animal; D005260:Female; D005313:Fetal Death / Q000209:etiology; D006566:Herpesviridae Infections / Q000150:complications; D007150:Immunohistochemistry; D011247:Pregnancy; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction; D018140:Varicellovirus
keywords: equine herpesvirus-9; pathogenicity; pregnancy; rat model