title: Cytotoxicity of probiotics from Philippine commercial dairy products on cancer cells and the effect on expression of cfos and cjun early apoptotic-promoting genes and Interleukin-1 β and Tumor Necrosis Factor-α proinflammatory cytokine genes.
abstract: This study determined cytotoxicity of probiotic Lactobacillus spp. from Philippine dairy products on cancer cells and normal fibroblasts and their effects on expression of early apoptotic-promoting cfos, cjun and proinflammatory cytokine IL-1β, TNF-α genes. Cultures were from Yakult, Bear Brand Probiotic Drink, Nido3+ Powdered Milk. Filter-sterilized supernatants from cultures of Lactobacillus spp. were evaluated for cytotoxicity to colon cancer cells (HT-29 and HCT116), leukemia cells (THP-1), and normal human dermal fibroblasts (HDFn) using PrestoBlue. Bleomycin was the positive control. Absolute quantification of transcript levels was conducted using qRT-PCR. Cytotoxicity index profiles on HDFn, THP-1 of all probiotic supernatants and negative controls suggest nontoxicity to the cells when compared to bleomycin, whereas all probiotic supernatants were found to be cytotoxic to HT-29 and HCT-116 colon cancer cell lines. Expression of cfos, cjun transcripts was significantly upregulated in HT-29 and HCT116 cells treated with probiotic supernatants compared to untreated baseline levels (P < 0.05). Expression of IL-1β and TNF-α by lipopolysaccharide-treated macrophages was significantly downregulated in cells with probiotic supernatants compared to those exposed to MRS medium (P < 0.05). Results provide strong support for the role of Lactobacillus spp. studied in anticancer therapy and in prevention of inflammation that may act as precursor to carcinogenesis.
authors.affiliation: Biology Department and Center for Natural Sciences and Ecological Research, De La Salle University, 2401 Taft Avenue, 1004 Manila, Philippines.
authors.forename: Peter T
authors.initials: PT
authors.lastname: Shyu
authors.affiliation: Biology Department and Center for Natural Sciences and Ecological Research, De La Salle University, 2401 Taft Avenue, 1004 Manila, Philippines.
authors.forename: Glenn G
authors.initials: GG
authors.lastname: Oyong
authors.affiliation: Biology Department and Center for Natural Sciences and Ecological Research, De La Salle University, 2401 Taft Avenue, 1004 Manila, Philippines.
authors.forename: Esperanza C
authors.initials: EC
authors.lastname: Cabrera
mesh_terms: D017209:Apoptosis / Q000235:genetics*; D015415:Biomarkers / Q000378:metabolism; D002460:Cell Line; D003611:Dairy Products / Q000382:microbiology*; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects*; D006801:Humans; D007249:Inflammation / Q000235:genetics*; D020128:Inhibitory Concentration 50; D053583:Interleukin-1beta / Q000235:genetics / Q000378:metabolism; D007778:Lactobacillus / Q000302:isolation & purification; D009369:Neoplasms / Q000235:genetics* / Q000473:pathology*; D010679:Philippines; D019936:Probiotics / Q000494:pharmacology*; D016760:Proto-Oncogene Proteins c-fos / Q000235:genetics / Q000378:metabolism; D016755:Proto-Oncogene Proteins c-jun / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000378:metabolism
chemical_list: D015415:Biomarkers; D053583:Interleukin-1beta; D016760:Proto-Oncogene Proteins c-fos; D016755:Proto-Oncogene Proteins c-jun; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha