title: Effect of graphitic layers encapsulating single-crystal apatite nanowire on the osteogenesis of human mesenchymal stem cells.
abstract: An ideally designed scaffold for tissue engineering must be able to provide an environment that recapitulates the physiological conditions to control stem cell function. Here, we compared vertically aligned single-crystal apatite nanowires sheathed in graphitic layers (SANGs) with single-crystal apatite nanowires (SANs), which had the same geometric properties as--but differing nanotopographic surface chemistry than--SANGs, in order to evaluate the effect of the graphitic layer on the behavior of human mesenchymal stem cells (hMSCs). The difference in nanotopographic surface chemistry did not affect hMSC adhesion, growth, or morphology. However, hMSCs were more effectively differentiated into bone cells on SANGs through interaction with graphitic layers, which later degraded and thereby allowed the cells to continue differentiation on the bare apatite nanowires. Thus, SANGs provide an excellent microenvironment for the osteogenic differentiation of hMCS.
authors.affiliation: Energy Materials, Convergence Research Department, Korea Institute of Energy Research , 71-2 Jang-dong, Yuseong-gu, Daejeon 305-343, Republic of Korea.
authors.forename: Namjo
authors.initials: N
authors.lastname: Jeong
authors.forename: Yun Chang
authors.initials: YC
authors.lastname: Park
authors.forename: Kyung Mee
authors.initials: KM
authors.lastname: Lee
authors.forename: Jae Hyup
authors.initials: JH
authors.lastname: Lee
authors.forename: Misun
authors.initials: M
authors.lastname: Cha
mesh_terms: D001031:Apatites / Q000737:chemistry*; D002118:Calcium / Q000378:metabolism; D002448:Cell Adhesion; D018929:Cell Culture Techniques / Q000295:instrumentation* / Q000379:methods; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D050659:Core Binding Factor Alpha 1 Subunit / Q000235:genetics / Q000378:metabolism; D006108:Graphite / Q000737:chemistry; D006801:Humans; D058574:Integrin-Binding Sialoprotein / Q000235:genetics / Q000378:metabolism; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D053770:Nanowires / Q000737:chemistry*; D015675:Osteocalcin / Q000235:genetics / Q000378:metabolism; D010012:Osteogenesis; D013499:Surface Properties; D023822:Tissue Engineering
chemical_list: D001031:Apatites; D050659:Core Binding Factor Alpha 1 Subunit; D058574:Integrin-Binding Sialoprotein; C493709:RUNX2 protein, human; D015675:Osteocalcin; D006108:Graphite; D002118:Calcium