title: Mass spectrometry-based metabolite profiling in the mouse liver following exposure to ultraviolet B radiation.
abstract: Although many studies have been performed on the effects of ultraviolet (UV) radiation on the skin, only a limited number of reports have investigated these effects on non-skin tissue. This study aimed to describe the metabolite changes in the liver of hairless mice following chronic exposure to UVB radiation. We did not observe significant macroscopic changes or alterations in hepatic cholesterol and triglyceride levels in the liver of UVB-irradiated mice, compared with those for normal mice. In this study, we detected hepatic metabolite changes by UVB exposure and identified several amino acids, fatty acids, nucleosides, carbohydrates, phospholipids, lysophospholipids, and taurine-conjugated cholic acids as candidate biomarkers in response to UVB radiation in the mouse liver by using various mass spectrometry (MS)-based metabolite profiling including ultra-performance liquid chromatography-quadrupole time-of-flight (TOF)-MS, gas chromatography-TOF-MS and nanomate LTQ-MS. Glutamine exhibited the most dramatic change with a 5-fold increase in quantity. The results from altering several types of metabolites suggest that chronic UVB irradiation may impact significantly on major hepatic metabolism processes, despite the fact that the liver is not directly exposed to UVB radiation. MS-based metabolomic approach for determining regulatory hepatic metabolites following UV irradiation will provide a better understanding of the relationship between internal organs and UV light.
authors.affiliation: Department of Bioscience and Biotechnology, Konkuk University, Gwangjin-gu, Seoul, Republic of Korea.
authors.forename: Hye Min
authors.initials: HM
authors.lastname: Park
authors.affiliation: College of Pharmacy and Research Institute of Pharmaceutical Sciences, Kyungpook National University, Buk-gu, Daegu, Republic of Korea.
authors.forename: Jong Cheol
authors.initials: JC
authors.lastname: Shon
authors.affiliation: Department of Bioscience and Biotechnology, Konkuk University, Gwangjin-gu, Seoul, Republic of Korea.
authors.forename: Mee Youn
authors.initials: MY
authors.lastname: Lee
authors.affiliation: College of Pharmacy and Research Institute of Pharmaceutical Sciences, Kyungpook National University, Buk-gu, Daegu, Republic of Korea.
authors.forename: Kwang-Hyeon
authors.initials: KH
authors.lastname: Liu
authors.affiliation: Food Research Institute, AmorePacific Corporation R&D Center, Giheung-gu, Yongin, Gyeonggi-do, Republic of Korea.
authors.forename: Jeong Kee
authors.initials: JK
authors.lastname: Kim
authors.affiliation: Food Research Institute, AmorePacific Corporation R&D Center, Giheung-gu, Yongin, Gyeonggi-do, Republic of Korea.
authors.forename: Sang Jun
authors.initials: SJ
authors.lastname: Lee
authors.affiliation: Department of Bioscience and Biotechnology, Konkuk University, Gwangjin-gu, Seoul, Republic of Korea.
authors.forename: Choong Hwan
authors.initials: CH
authors.lastname: Lee
mesh_terms: D000818:Animals; D005260:Female; D008099:Liver / Q000378:metabolism* / Q000528:radiation effects*; D013058:Mass Spectrometry / Q000379:methods*; D055442:Metabolome / Q000528:radiation effects*; D055432:Metabolomics / Q000379:methods*; D008812:Mice, Hairless; D012867:Skin; D014466:Ultraviolet Rays