title: Transcriptome reconstruction and annotation of cynomolgus and African green monkey.
abstract: BACKGROUND
  Non-human primates (NHPs) and humans share major biological mechanisms, functions, and responses due to their close evolutionary relationship and, as such, provide ideal animal models to study human diseases. RNA expression in NHPs provides specific signatures that are informative of disease mechanisms and therapeutic modes of action. Unlike the human transcriptome, the transcriptomes of major NHP animal models are yet to be comprehensively annotated.
  
  
  RESULTS
  In this manuscript, employing deep RNA sequencing of seven tissue samples, we characterize the transcriptomes of two commonly used NHP animal models: Cynomolgus macaque (Macaca fascicularis) and African green monkey (Chlorocebus aethiops). We present the Multi-Species Annotation (MSA) pipeline that leverages well-annotated primate species and annotates 99.8% of reconstructed transcripts. We elucidate tissue-specific expression profiles and report 13 experimentally validated novel transcripts in these NHP animal models.
  
  
  CONCLUSIONS
  We report comprehensively annotated transcriptomes of two non-human primates, which we have made publically available on a customized UCSC Genome Browser interface. The MSA pipeline is also freely available.
authors.forename: Albert
authors.initials: A
authors.lastname: Lee
authors.forename: Hossein
authors.initials: H
authors.lastname: Khiabanian
authors.forename: Jeffrey
authors.initials: J
authors.lastname: Kugelman
authors.forename: Oliver
authors.initials: O
authors.lastname: Elliott
authors.forename: Elyse
authors.initials: E
authors.lastname: Nagle
authors.forename: Guo-Yun
authors.initials: GY
authors.lastname: Yu
authors.forename: Travis
authors.initials: T
authors.lastname: Warren
authors.forename: Gustavo
authors.initials: G
authors.lastname: Palacios
authors.affiliation: Department of Biomedical Informatics, Columbia University College of Physicians and Surgeons, New York, New York, NY 10032, USA. rabadan@c2b2.columbia.edu.
authors.forename: Raul
authors.initials: R
authors.lastname: Rabadan
mesh_terms: D000818:Animals; D002522:Chlorocebus aethiops / Q000235:genetics*; D055028:Comparative Genomic Hybridization; D005091:Exons; D020869:Gene Expression Profiling; D016678:Genome; D059014:High-Throughput Nucleotide Sequencing; D008252:Macaca fascicularis / Q000235:genetics*; D058977:Molecular Sequence Annotation; D017423:Sequence Analysis, RNA; D059467:Transcriptome