title: Permanent draft genome sequence of Bacillus flexus strain T6186-2, a multidrug-resistant bacterium isolated from a deep-subsurface oil reservoir.
abstract: Previous studies suggest that antibiotic resistance genes have an ancient origin, which is not always linked to the use of antibiotics but can be enhanced by human activities. Bacillus flexus strain T6186-2 was isolated from the formation water sample of a deep-subsurface oil reservoir. Interestingly, antimicrobial susceptibility testing showed that this strain is susceptible to kanamycin, however, resistant to ampicillin, erythromycin, gentamicin, vancomycin, fosfomycin, fosmidomycin, tetracycline and teicoplanin. To explore our knowledge about the origins of antibiotic resistance genes (ARGs) in the relatively pristine environment, we sequenced the genome of B. flexus strain T6186-2 as a permanent draft. It represents the evidence for the existence of a reservoir of ARGs in nature among microbial populations from deep-subsurface oil reservoirs.
authors.affiliation: The Key Laboratory of Marine Reservoir Evolution and Hydrocarbon Accumulation Mechanism, Ministry of Education, School of Energy Resources, China University of Geosciences, Beijing, China.
authors.forename: Fan
authors.initials: F
authors.lastname: Zhang
authors.affiliation: State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, Zhejiang University, Hangzhou, China.
authors.forename: Xiawei
authors.initials: X
authors.lastname: Jiang
authors.affiliation: The Key Laboratory of Marine Reservoir Evolution and Hydrocarbon Accumulation Mechanism, Ministry of Education, School of Energy Resources, China University of Geosciences, Beijing, China.
authors.forename: Lujun
authors.initials: L
authors.lastname: Chai
authors.affiliation: College of Chemistry and Environmental Engineering, Yangtze University, Jingzhou, China. Electronic address: sheyuehui@263.net.
authors.forename: Yuehui
authors.initials: Y
authors.lastname: She
authors.affiliation: College of Petroleum Engineering, Yangtze University, Jingzhou, China.
authors.forename: Gaoming
authors.initials: G
authors.lastname: Yu
authors.affiliation: College of Chemistry and Environmental Engineering, Yangtze University, Jingzhou, China.
authors.forename: Fuchang
authors.initials: F
authors.lastname: Shu
authors.affiliation: College of Chemistry and Environmental Engineering, Yangtze University, Jingzhou, China.
authors.forename: Zhengliang
authors.initials: Z
authors.lastname: Wang
authors.affiliation: College of Chemistry and Environmental Engineering, Yangtze University, Jingzhou, China.
authors.forename: Sanbao
authors.initials: S
authors.lastname: Su
authors.affiliation: College of Chemistry and Environmental Engineering, Yangtze University, Jingzhou, China.
authors.forename: Wu
authors.initials: W
authors.lastname: Wenqiong
authors.affiliation: College of Chemistry and Environmental Engineering, Yangtze University, Jingzhou, China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Tingsheng
authors.affiliation: State Key Laboratory of Heavy Oil Processing, China University of Petroleum, Beijing, China.
authors.forename: Zhongzhi
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: The Key Laboratory of Marine Reservoir Evolution and Hydrocarbon Accumulation Mechanism, Ministry of Education, School of Energy Resources, China University of Geosciences, Beijing, China.
authors.forename: Dujie
authors.initials: D
authors.lastname: Hou
authors.affiliation: State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, Zhejiang University, Hangzhou, China. Electronic address: zhengbw@zju.edu.cn.
authors.forename: Beiwen
authors.initials: B
authors.lastname: Zheng
mesh_terms: D001407:Bacillus / Q000235:genetics*; D001482:Base Composition; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics*; D016680:Genome, Bacterial / Q000235:genetics*; D060587:Groundwater / Q000382:microbiology*; D008969:Molecular Sequence Data; D061329:Oil and Gas Fields / Q000382:microbiology*; D017422:Sequence Analysis, DNA
chemical_list: D017931:DNA Primers
keywords: Antibiotic resistance genes; Bacillus flexus; Genome; Halotolerant; Oilfield