title: In vitro cytotoxicity of self-curing acrylic resins of different colors.
abstract: OBJECTIVE
  The aim of this study was to assess the in vitro cytotoxicity of acrylic resins of different colors over time.
  
  
  METHODS
  Specimens were divided into 4 groups (n = 6) according to the color of the acrylic resin (Orto Class, Clássico, Campinas, São Paulo, Brazil): Group 1, clear acrylic resin; Group 2, pink acrylic resin; Group 3, blue acrylic resin; and Group 4, green acrylic resin. All specimens were fabricated according to the mass manipulation technique and submitted to mechanical polishing protocol. The control was performed with an amalgam specimen (C+), a glass specimen (C-) and cell control (CC). Specimens were immersed in Minimum Eagle's Medium (MEM) and incubated for 24 h at 37ºC. The extracts from the experimental material were filtered and mixed with L929 fibroblast. Cytotoxicity was evaluated at four different times, 24, 48, 72 and 168 h. After contact, cells were incubated for 24 h and added to 100 µ of 0.01% neutral red dye. The cells were incubated for 3 h for pigment incorporation and fixed. Cells viability was determined by a spectroscopic (BioTek, Winooski, Vermont, USA) with a 492-nm wavelength λ=492 nm).
  
  
  RESULTS
  There were no statistical differences between the experimental groups and the CC and C- groups.
  
  
  CONCLUSIONS
  Clear, pink, blue and green self-curing acrylic resins fabricated by means of the mass manipulation technique and mechanically polished are not cytotoxic. Neither the pigment added to the self-curing acrylic resin nor the factor of time influenced the cytotoxicity of the material.
authors.forename: Luciana Borges
authors.initials: LB
authors.lastname: Retamoso
authors.forename: Taís de Morais Alves
authors.initials: Tde M
authors.lastname: da Cunha
authors.forename: Matheus Melo
authors.initials: MM
authors.lastname: Pithon
authors.forename: Rogério Lacerda
authors.initials: RL
authors.lastname: dos Santos
authors.forename: Fernanda Otaviano
authors.initials: FO
authors.lastname: Martins
authors.forename: Maria Teresa Villela
authors.initials: MT
authors.lastname: Romanos
authors.forename: Orlando Motohiro
authors.initials: OM
authors.lastname: Tanaka
mesh_terms: D000180:Acrylic Resins / Q000633:toxicity*; D000818:Animals; D018929:Cell Culture Techniques; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D003116:Color; D004396:Coloring Agents / Q000633:toxicity*; D003723:Dental Amalgam / Q000633:toxicity; D003764:Dental Materials / Q000633:toxicity*; D003775:Dental Polishing / Q000379:methods; D005347:Fibroblasts / Q000187:drug effects; D005898:Glass / Q000737:chemistry; D007202:Indicators and Reagents; D008422:Materials Testing; D051379:Mice; D009499:Neutral Red; D058105:Polymerization; D055116:Self-Curing of Dental Resins / Q000379:methods; D013057:Spectrum Analysis; D013499:Surface Properties; D013696:Temperature; D013997:Time Factors
chemical_list: D000180:Acrylic Resins; D004396:Coloring Agents; D003764:Dental Materials; D007202:Indicators and Reagents; C463899:Orthoresin; D009499:Neutral Red; D003723:Dental Amalgam
keywords: Acrylic resins; Cell culture techniques; Cytotoxins