title: The expression of p21 is upregulated by forkhead box A1/2 in p53-null H1299 cells.
abstract: The expression of the cell cycle inhibitor p21 is increased in response to various stimuli and stress signals through p53-dependent and independent pathways. We demonstrate in this study that forkhead box A1/2 (FOXA1/2) is a crucial transcription factor in the activation of p21 transcription via direct binding to the p21 promoter in p53-null H1299 lung carcinoma cells. In addition, histone deacetylase inhibitor trichostatin A (TSA)-mediated upregulation of p21 expression was repressed by knockdown of FOXA1/2 in H1299 cells. Consequently, these results suggest that FOXA1/2 is required for p53-independent p21 expression.
authors.affiliation: Department of Life Science, College of Natural Sciences, Chung-Ang University, Seoul 156-756, Republic of Korea.
authors.forename: Joo-Hee
authors.initials: JH
authors.lastname: An
authors.affiliation: Department of Life Science, College of Natural Sciences, Chung-Ang University, Seoul 156-756, Republic of Korea.
authors.forename: Sang-Min
authors.initials: SM
authors.lastname: Jang
authors.affiliation: Department of Life Science, College of Natural Sciences, Chung-Ang University, Seoul 156-756, Republic of Korea; Neurobiology-Neurodegeneration and Repair Laboratory, NEI, National Institutes of Health, Bethesda, MD 20892, USA.
authors.forename: Jung-Woong
authors.initials: JW
authors.lastname: Kim
authors.affiliation: Department of Life Science, College of Natural Sciences, Chung-Ang University, Seoul 156-756, Republic of Korea.
authors.forename: Chul-Hong
authors.initials: CH
authors.lastname: Kim
authors.affiliation: Department of Dermatology, University of Colorado Denver Anschutz Medical Campus, Aurora, CO 80045, USA. Electronic address: PETER.SONG@ucdenver.edu.
authors.forename: Peter I
authors.initials: PI
authors.lastname: Song
authors.affiliation: Department of Life Science, College of Natural Sciences, Chung-Ang University, Seoul 156-756, Republic of Korea. Electronic address: khchoi@cau.ac.kr.
authors.forename: Kyung-Hee
authors.initials: KH
authors.lastname: Choi
mesh_terms: D001483:Base Sequence; D045744:Cell Line, Tumor; D050759:Cyclin-Dependent Kinase Inhibitor p21 / Q000235:genetics*; D017353:Gene Deletion; D051541:Hepatocyte Nuclear Factor 3-alpha / Q000378:metabolism*; D051542:Hepatocyte Nuclear Factor 3-beta / Q000378:metabolism*; D006801:Humans; D006877:Hydroxamic Acids / Q000494:pharmacology; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic / Q000187:drug effects / Q000235:genetics; D014158:Transcription, Genetic / Q000187:drug effects; D015533:Transcriptional Activation / Q000187:drug effects; D016159:Tumor Suppressor Protein p53 / Q000172:deficiency* / Q000235:genetics*; D015854:Up-Regulation / Q000187:drug effects
chemical_list: D050759:Cyclin-Dependent Kinase Inhibitor p21; C496499:FOXA1 protein, human; C496502:FOXA2 protein, human; D051541:Hepatocyte Nuclear Factor 3-alpha; D006877:Hydroxamic Acids; D016159:Tumor Suppressor Protein p53; D051542:Hepatocyte Nuclear Factor 3-beta; C012589:trichostatin A
keywords: Target gene; Transcription factor FOXA1/2; Trichostatin A; p21; p53-Independent pathway