title: Osteoblast activity of MG-63 cells is enhanced by growth on a lactoferrin-immobilized titanium substrate.
abstract: The aim of this study was to develop a lactoferrin (LF)-immobilized titanium (Ti) substrate to enhance the osteoblast activity of MG-63 cells. Ti substrates were first modified through heparin-dopamine (Hep-DOPA) anchorage. Then, LF was immobilized on the Hep-Ti substrates via electrostatic interactions. Hep-Ti substrates, with or without LF, were evaluated by scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS), and contact angle measurements. Sustained release of LF on the Ti substrates was observed over a 28-day period. In vitro studies of osteoblast activity showed increased alkaline phosphatase activity and calcium deposition by MG-63 cells cultured on LF-immobilized Ti substrates as compared to those cultured on pristine Ti substrates, indicating that LF-immobilized Ti substrates were effective at enhancing osteoblast activity.
authors.affiliation: Department of Orthopedic Surgery and Rare Diseases Institute, Korea University Medical College, Guro Hospital, #80, Guro-dong, Guro-gu, Seoul 152-703, Republic of Korea.
authors.forename: Sung Eun
authors.initials: SE
authors.lastname: Kim
authors.affiliation: Department of Orthopedic Surgery and Rare Diseases Institute, Korea University Medical College, Guro Hospital, #80, Guro-dong, Guro-gu, Seoul 152-703, Republic of Korea.
authors.forename: Young-Pil
authors.initials: YP
authors.lastname: Yun
authors.affiliation: Department of Biomedical Science, College of Medicine, Korea University, Anam-dong, Seongbuk-gu 136-701, Republic of Korea.
authors.forename: Jae Yong
authors.initials: JY
authors.lastname: Lee
authors.affiliation: Division of Bio-imaging, Chuncheon Center, Korea Basic Science Institute, 192-1 Hyoja 2-dong, Chuncheon, Gangwon-do 200-701, Republic of Korea.
authors.forename: Kyeongsoon
authors.initials: K
authors.lastname: Park
authors.affiliation: Department of Orthopedic Surgery, Korea University Medical College, Ansan Hospital, Gojan 1-dong, Danwon-gu, Gyeonggi-do 425-707, Republic of Korea. Electronic address: mayhap@hanmail.net.
authors.forename: Dong Hun
authors.initials: DH
authors.lastname: Suh
mesh_terms: D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D004298:Dopamine / Q000737:chemistry; D006493:Heparin / Q000737:chemistry; D006801:Humans; D007781:Lactoferrin / Q000737:chemistry* / Q000494:pharmacology*; D010006:Osteoblasts / Q000166:cytology* / Q000187:drug effects*; D014025:Titanium / Q000737:chemistry*
chemical_list: D006493:Heparin; D014025:Titanium; D007781:Lactoferrin; D004298:Dopamine
keywords: Heparin–dopamine; Lactoferrin; Osteoblast; Osteogenic differentiation; Titanium