title: Bodipy-labeled nucleoside triphosphates for polymerase synthesis of fluorescent DNA.
abstract: New fluorescent nucleosides and nucleoside triphosphate (dNTPs) analogs bearing the F-Bodipy fluorophore linked through a short, flexible nonconjugate tether were synthesized. The Bodipy-labeled dNTPs were substrates for several DNA polymerases which incorporated them into DNA in primer extension, nicking enzyme amplification reaction, and polymerase chain reaction. The fluorescence of F-Bodipy is not quenched upon incorporation in DNA and can be detected both in solutions and on gels.
authors.affiliation: Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, Gilead & IOCB Research Center , Flemingovo nam. 2, CZ-16610 Prague 6, Czech Republic.
authors.forename: Dmytro
authors.initials: D
authors.lastname: Dziuba
authors.forename: Radek
authors.initials: R
authors.lastname: Pohl
authors.forename: Michal
authors.initials: M
authors.lastname: Hocek
mesh_terms: D001483:Base Sequence; D001896:Boron Compounds / Q000737:chemistry*; D060326:Chemistry Techniques, Synthetic; D004247:DNA / Q000138:chemical synthesis* / Q000737:chemistry* / Q000235:genetics; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism*; D005456:Fluorescent Dyes / Q000737:chemistry*; D009711:Nucleotides / Q000737:chemistry*; D011122:Polyphosphates / Q000737:chemistry*
chemical_list: C095489:4,4-difluoro-4-bora-3a,4a-diaza-s-indacene; D001896:Boron Compounds; D005456:Fluorescent Dyes; D009711:Nucleotides; D011122:Polyphosphates; D004247:DNA; D004259:DNA-Directed DNA Polymerase; C005692:triphosphoric acid