title: A receptor-like protein mediates the response to pectin modification by activating brassinosteroid signaling.
abstract: The brassinosteroid (BR) signaling module is a central regulator of plant morphogenesis, as indicated by the large number of BR-responsive cell wall-related genes and the severe growth defects of BR mutants. Despite a detailed knowledge of the signaling components, the logic of this auto-/paracrine signaling module in growth control remains poorly understood. Recently, extensive cross-talk with other signaling pathways has been shown, suggesting that the outputs of BR signaling, such as gene-expression changes, are subject to complex control mechanisms. We previously provided evidence for a role of BR signaling in a feedback loop controlling the integrity of the cell wall. Here, we identify the first dedicated component of this feedback loop: a receptor-like protein (RLP44), which is essential for the compensatory triggering of BR signaling upon inhibition of pectin de-methylesterification in the cell wall. RLP44 is required for normal growth and stress responses and connects with the BR signaling pathway, presumably through a direct interaction with the regulatory receptor-like kinase BAK1. These findings corroborate a role for BR in controlling the sensitivity of a feedback signaling module involved in maintaining the physico-chemical homeostasis of the cell wall during cell expansion.
authors.affiliation: Centre for Organismal Studies, University of Heidelberg, 69120 Heidelberg, Germany; Saclay Plant Science, Institut Jean-Pierre Bourgin, Unité Mixte de Recherche 1318, Institut National de la Recherche Agronomique-AgroParisTech, 78026 Versailles, France; sebastian.wolf@cos.uni-heidelberg.de herman.hofte@versailles.inra.fr.
authors.forename: Sebastian
authors.initials: S
authors.lastname: Wolf
authors.affiliation: The Sainsbury Laboratory, Norwich Research Park, Norwich NR4 7UH, United Kingdom;
authors.forename: Dieuwertje
authors.initials: D
authors.lastname: van der Does
authors.affiliation: Department for Plant Physiology, Center for Plant Molecular Biology, University of Tübingen, 72076 Tübingen, Germany; and.
authors.forename: Friederike
authors.initials: F
authors.lastname: Ladwig
authors.affiliation: Zentrum für Medizinische Forschung, Medizinische Fakultät Mannheim, 68167 Mannheim, Germany.
authors.forename: Carsten
authors.initials: C
authors.lastname: Sticht
authors.affiliation: Centre for Organismal Studies, University of Heidelberg, 69120 Heidelberg, Germany;
authors.forename: Andreas
authors.initials: A
authors.lastname: Kolbeck
authors.affiliation: Centre for Organismal Studies, University of Heidelberg, 69120 Heidelberg, Germany;
authors.forename: Ann-Kathrin
authors.initials: AK
authors.lastname: Schürholz
authors.affiliation: Centre for Organismal Studies, University of Heidelberg, 69120 Heidelberg, Germany;
authors.forename: Sebastian
authors.initials: S
authors.lastname: Augustin
authors.affiliation: Centre for Organismal Studies, University of Heidelberg, 69120 Heidelberg, Germany;
authors.forename: Nana
authors.initials: N
authors.lastname: Keinath
authors.affiliation: Centre for Organismal Studies, University of Heidelberg, 69120 Heidelberg, Germany;
authors.forename: Thomas
authors.initials: T
authors.lastname: Rausch
authors.affiliation: Centre for Organismal Studies, University of Heidelberg, 69120 Heidelberg, Germany;
authors.forename: Steffen
authors.initials: S
authors.lastname: Greiner
authors.affiliation: Centre for Organismal Studies, University of Heidelberg, 69120 Heidelberg, Germany;
authors.forename: Karin
authors.initials: K
authors.lastname: Schumacher
authors.affiliation: Department for Plant Physiology, Center for Plant Molecular Biology, University of Tübingen, 72076 Tübingen, Germany; and.
authors.forename: Klaus
authors.initials: K
authors.lastname: Harter
authors.affiliation: The Sainsbury Laboratory, Norwich Research Park, Norwich NR4 7UH, United Kingdom;
authors.forename: Cyril
authors.initials: C
authors.lastname: Zipfel
authors.affiliation: Saclay Plant Science, Institut Jean-Pierre Bourgin, Unité Mixte de Recherche 1318, Institut National de la Recherche Agronomique-AgroParisTech, 78026 Versailles, France; sebastian.wolf@cos.uni-heidelberg.de herman.hofte@versailles.inra.fr.
authors.forename: Herman
authors.initials: H
authors.lastname: Höfte
mesh_terms: D029681:Arabidopsis Proteins / Q000502:physiology; D060406:Brassinosteroids / Q000737:chemistry*; D002473:Cell Wall / Q000378:metabolism; D003001:Cloning, Molecular; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D006706:Homeostasis; D008024:Ligands; D018613:Microscopy, Confocal; D009154:Mutation; D010368:Pectins / Q000737:chemistry*; D010641:Phenotype; D010940:Plant Proteins / Q000502:physiology*; D011485:Protein Binding; D025941:Protein Interaction Mapping; D017346:Protein Serine-Threonine Kinases / Q000502:physiology; D015398:Signal Transduction
chemical_list: D029681:Arabidopsis Proteins; D060406:Brassinosteroids; D008024:Ligands; D010940:Plant Proteins; D010368:Pectins; C463660:BAK1 protein, Arabidopsis; D017346:Protein Serine-Threonine Kinases
keywords: brassinosteroids; cell wall integrity; pectin