title: Sirtuin 3 deficiency is associated with inhibited mitochondrial function and pulmonary arterial hypertension in rodents and humans.
abstract: Suppression of mitochondrial function promoting proliferation and apoptosis suppression has been described in the pulmonary arteries and extrapulmonary tissues in pulmonary arterial hypertension (PAH), but the cause of this metabolic remodeling is unknown. Mice lacking sirtuin 3 (SIRT3), a mitochondrial deacetylase, have increased acetylation and inhibition of many mitochondrial enzymes and complexes, suppressing mitochondrial function. Sirt3KO mice develop spontaneous PAH, exhibiting previously described molecular features of PAH pulmonary artery smooth muscle cells (PASMC). In human PAH PASMC and rats with PAH, SIRT3 is downregulated, and its normalization with adenovirus gene therapy reverses the disease phenotype. A loss-of-function SIRT3 polymorphism, linked to metabolic syndrome, is associated with PAH in an unbiased cohort of 162 patients and controls. If confirmed in large patient cohorts, these findings may facilitate biomarker and therapeutic discovery programs in PAH.
authors.affiliation: Department of Medicine, University of Alberta, Edmonton, AB T6G 2B7, Canada.
authors.forename: Roxane
authors.initials: R
authors.lastname: Paulin
authors.affiliation: Department of Medicine, University of Alberta, Edmonton, AB T6G 2B7, Canada.
authors.forename: Peter
authors.initials: P
authors.lastname: Dromparis
authors.affiliation: Department of Medicine, University of Alberta, Edmonton, AB T6G 2B7, Canada.
authors.forename: Gopinath
authors.initials: G
authors.lastname: Sutendra
authors.affiliation: Department of Medicine, University of Alberta, Edmonton, AB T6G 2B7, Canada.
authors.forename: Vikram
authors.initials: V
authors.lastname: Gurtu
authors.affiliation: Department of Medicine, University of Alberta, Edmonton, AB T6G 2B7, Canada.
authors.forename: Sotirios
authors.initials: S
authors.lastname: Zervopoulos
authors.affiliation: Department of Medicine, University of Alberta, Edmonton, AB T6G 2B7, Canada.
authors.forename: Lyndsay
authors.initials: L
authors.lastname: Bowers
authors.affiliation: Department of Medicine, University of Alberta, Edmonton, AB T6G 2B7, Canada.
authors.forename: Alois
authors.initials: A
authors.lastname: Haromy
authors.affiliation: Department of Medicine, University of Alberta, Edmonton, AB T6G 2B7, Canada.
authors.forename: Linda
authors.initials: L
authors.lastname: Webster
authors.affiliation: Department of Medicine, Laval University, IUCPQ Research Centre, Pulmonary Hypertension Research Group, Quebec, QC G1V 4G5, Canada.
authors.forename: Steeve
authors.initials: S
authors.lastname: Provencher
authors.affiliation: Department of Medicine, Laval University, IUCPQ Research Centre, Pulmonary Hypertension Research Group, Quebec, QC G1V 4G5, Canada.
authors.forename: Sebastien
authors.initials: S
authors.lastname: Bonnet
authors.affiliation: Department of Medicine, University of Alberta, Edmonton, AB T6G 2B7, Canada. Electronic address: em2@ualberta.ca.
authors.forename: Evangelos D
authors.initials: ED
authors.lastname: Michelakis
mesh_terms: D000328:Adult; D000818:Animals; D002478:Cells, Cultured; D015536:Down-Regulation; D065627:Familial Primary Pulmonary Hypertension / Q000235:genetics / Q000473:pathology / Q000628:therapy; D005260:Female; D015316:Genetic Therapy; D006801:Humans; D006976:Hypertension, Pulmonary / Q000235:genetics* / Q000473:pathology* / Q000628:therapy; D008168:Lung / Q000098:blood supply* / Q000378:metabolism / Q000473:pathology; D051379:Mice; D018345:Mice, Knockout; D008875:Middle Aged; D008928:Mitochondria / Q000378:metabolism / Q000473:pathology*; D011110:Polymorphism, Genetic; D011651:Pulmonary Artery / Q000378:metabolism / Q000473:pathology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D056566:Sirtuin 3 / Q000235:genetics*
chemical_list: D056566:Sirtuin 3