title: Poly (lactic acid)/chitosan fiber mats: investigation of effects of the support on lipase immobilization.
abstract: The greatest challenge for biotechnological processes is to have immobilized enzymes acting as good green catalysts with high reusability rates. In this work, we have produced electrospun fibers from poly (lactic acid)/chitosan blends. Further, we evaluated the influence of these materials as support for lipase immobilization. The PLA/chitosan fiber mats were composed by non-woven nanofibers, with diameters ranging from 200 nm to 1.3 μm. The solvent (TFA) as well as the chitosan addition influenced morphology, hydrophobicity and mechanical properties of PLA nanofibers. It was observed that even for lower concentration of lipase (5 U) higher enzyme activity retention was detected in the PLA/chitosan blends. In addition, a remarkable improvement of lipase activity on pure PLA fiber mat was verified, indicating that most of the enzymes were probably in their active form.
authors.affiliation: Laboratory of Advanced Polymers, Institute of Chemistry, Universidade Federal do Rio Grande do Sul, Av. Bento Gonçalves, 9500, Lab K-208 A, Porto Alegre, RS CEP 91501-970, Brazil; Post-Graduate Program of Materials Science, Universidade Federal do Rio Grande do Sul (UFRGS), Av. Bento Gonçalves, 9500, Sala A216, Porto Alegre, RS CEP 91501-970, Brazil.
authors.forename: Nataly M
authors.initials: NM
authors.lastname: Siqueira
authors.affiliation: Laboratory of Advanced Polymers, Institute of Chemistry, Universidade Federal do Rio Grande do Sul, Av. Bento Gonçalves, 9500, Lab K-208 A, Porto Alegre, RS CEP 91501-970, Brazil.
authors.forename: Ketlin C
authors.initials: KC
authors.lastname: Garcia
authors.affiliation: Laboratory of Fungi Importance Medicine and Biotechnology, Institute of Biotechnology, Universidade Federal do Rio Grande do Sul, Lab 220, Av. Bento Gonçalves, 9500, Porto Alegre, RS CEP 91501-970, Brazil.
authors.forename: Roberta
authors.initials: R
authors.lastname: Bussamara
authors.affiliation: Laboratory of Fungi Importance Medicine and Biotechnology, Institute of Biotechnology, Universidade Federal do Rio Grande do Sul, Lab 220, Av. Bento Gonçalves, 9500, Porto Alegre, RS CEP 91501-970, Brazil.
authors.forename: Fernanda S
authors.initials: FS
authors.lastname: Both
authors.affiliation: Laboratory of Fungi Importance Medicine and Biotechnology, Institute of Biotechnology, Universidade Federal do Rio Grande do Sul, Lab 220, Av. Bento Gonçalves, 9500, Porto Alegre, RS CEP 91501-970, Brazil.
authors.forename: Marilene H
authors.initials: MH
authors.lastname: Vainstein
authors.affiliation: Laboratory of Advanced Polymers, Institute of Chemistry, Universidade Federal do Rio Grande do Sul, Av. Bento Gonçalves, 9500, Lab K-208 A, Porto Alegre, RS CEP 91501-970, Brazil; Post-Graduate Program of Materials Science, Universidade Federal do Rio Grande do Sul (UFRGS), Av. Bento Gonçalves, 9500, Sala A216, Porto Alegre, RS CEP 91501-970, Brazil. Electronic address: soaresr@ufrgs.br.
authors.forename: Rosane M D
authors.initials: RM
authors.lastname: Soares
mesh_terms: D048271:Chitosan / Q000138:chemical synthesis / Q000737:chemistry*; D004800:Enzymes, Immobilized / Q000737:chemistry; D019344:Lactic Acid / Q000138:chemical synthesis / Q000737:chemistry*; D008049:Lipase / Q000737:chemistry* / Q000378:metabolism; D057139:Nanofibers / Q000737:chemistry*; D011091:Polyesters; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry*; D013499:Surface Properties; D013718:Tensile Strength / Q000187:drug effects; D023822:Tissue Engineering
chemical_list: D004800:Enzymes, Immobilized; D011091:Polyesters; D011108:Polymers; D019344:Lactic Acid; C033616:poly(lactide); D048271:Chitosan; D008049:Lipase
keywords: Chitosan; Electrospinning; Immobilization; Lipase; Nanofibers; poly (lactic acid)