title: Synergistic co-delivery of doxorubicin and paclitaxel by porous PLGA microspheres for pulmonary inhalation treatment.
abstract: PLGA porous microspheres loaded with doxorubicin (DOX) and paclitaxel (PTX) were developed for in situ treatment of metastatic lung cancer. The synergistic effect of the combined drugs was investigated against B16F10 cells to obtain the optimal prescription for in vivo studies. The combination therapy showed great synergism when DOX was the majority in the combination therapy, while they showed moderate antagonism when PTX is in major. The combination of DOX and PTX at a molar ratio of 5/1 showed the best synergistic therapeutic effect in the free form. However, the drugs exhibited more synergism in the PLGA microspheres at a molar ratio of 2/1, due to the difference in drug release rate. The in vivo study verified the synergism of DOX and PTX at the optimal molar ratio. These results suggested that dual encapsulation of DOX and PTX in porous PLGA microspheres would be a promising technology for long effective lung cancer treatment.
authors.affiliation: CAS Key Laboratory of Soft Matter Chemistry, Department of Polymer Science and Engineering, University of Science and Technology of China, Hefei, China; Key Laboratory of Polymer Ecomaterials, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, China; Department of Biology and Chemistry, City University of Hong Kong, Hong Kong, China; Advanced Laboratory of Environmental Research and Technology (ALERT), Joint Advanced Research Center, Suzhou, China.
authors.forename: Tianshi
authors.initials: T
authors.lastname: Feng
authors.affiliation: Key Laboratory of Polymer Ecomaterials, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, China. Electronic address: thy@ciac.ac.cn.
authors.forename: Huayu
authors.initials: H
authors.lastname: Tian
authors.affiliation: Key Laboratory of Polymer Ecomaterials, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, China.
authors.forename: Caina
authors.initials: C
authors.lastname: Xu
authors.affiliation: Key Laboratory of Polymer Ecomaterials, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Lin
authors.affiliation: Key Laboratory of Polymer Ecomaterials, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, China.
authors.forename: Zhigang
authors.initials: Z
authors.lastname: Xie
authors.affiliation: Department of Biology and Chemistry, City University of Hong Kong, Hong Kong, China; Advanced Laboratory of Environmental Research and Technology (ALERT), Joint Advanced Research Center, Suzhou, China.
authors.forename: Michael Hon-Wah
authors.initials: MH
authors.lastname: Lam
authors.affiliation: CAS Key Laboratory of Soft Matter Chemistry, Department of Polymer Science and Engineering, University of Science and Technology of China, Hefei, China; Advanced Laboratory of Environmental Research and Technology (ALERT), Joint Advanced Research Center, Suzhou, China.
authors.forename: Haojun
authors.initials: H
authors.lastname: Liang
authors.affiliation: Key Laboratory of Polymer Ecomaterials, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, China.
authors.forename: Xuesi
authors.initials: X
authors.lastname: Chen
mesh_terms: D000280:Administration, Inhalation; D000818:Animals; D000970:Antineoplastic Agents / Q000008:administration & dosage / Q000378:metabolism; D004317:Doxorubicin / Q000008:administration & dosage* / Q000378:metabolism; D004337:Drug Carriers / Q000008:administration & dosage / Q000378:metabolism; D016503:Drug Delivery Systems / Q000379:methods*; D004357:Drug Synergism; D019344:Lactic Acid / Q000008:administration & dosage* / Q000378:metabolism; D008168:Lung / Q000187:drug effects / Q000378:metabolism; D008175:Lung Neoplasms / Q000188:drug therapy* / Q000378:metabolism; D008546:Melanoma, Experimental; D051379:Mice; D008810:Mice, Inbred C57BL; D008863:Microspheres; D017239:Paclitaxel / Q000008:administration & dosage* / Q000378:metabolism; D011100:Polyglycolic Acid / Q000008:administration & dosage* / Q000378:metabolism; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D016062:Porosity; D011897:Random Allocation; D016896:Treatment Outcome; D023041:Xenograft Model Antitumor Assays / Q000379:methods
chemical_list: D000970:Antineoplastic Agents; D004337:Drug Carriers; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D004317:Doxorubicin; D017239:Paclitaxel
keywords: Doxorubicin; In situ treatment; Paclitaxel; Porous microspheres; Pulmonary inhalation; Synergistic effect