title: The type of ploidy of chrysanthemum is not black or white: a comparison of a molecular approach to published cytological methods.
abstract: Polyploidy is a widespread phenomenon among higher plants and a major factor shaping the structure and evolution of plant genomes. The important ornamental chrysanthemum (Chrysanthemum indicum hybrid) possesses a hexaploid genome with 54 chromosomes and was classified based on its evolutionary origin and cytological methods as an allopolyploid. However, it is questionable whether cytological methods are sufficient to determine the type of ploidy, and there are more informative methods available based on molecular marker analyses. Therefore, we collected segregation data for 406 dominant molecular marker alleles [327 amplified fragment length polymorphism (AFLPs), 65 single-strand conformation polymorphism (SSCPs) and 14 microsatellites (EST-SSRs)] in a biparental F1 population of 160 individuals. We analyzed these data for the characteristics that differ between allopolyploids and autopolyploids, including the segregation ratio of each marker, the ratio of single-dose (SD) to multi-dose (MD) markers, the ratio of SD markers in coupling to those in repulsion and the banding patterns of the SSRs. Whereas the analysis of the segregation ratio of each polymorphic marker indicated disomic (13 markers) as well as hexasomic (eight markers) inheritance, the ratio of SD markers in coupling to those in repulsion was 1:0, which is characteristic of autopolyploids. The observed ratio of SD to MD markers was 0.67:0.33 which is significantly different to the expected segregation for auto- and allohexaploids. Furthermore, the three EST-SSR alleles were inherited in all possible combinations and were not independent of each other, as expected for fixed heterozygosity in allopolyploids. Combining our results with published cytological data indicates that cultivated chrysanthemums should be classified as segmental allohexaploids.
authors.affiliation: Department of Molecular Plant Breeding, Institute for Plant Genetics, Leibniz Universität Hannover Hannover, Germany ; Kleinwanzlebener Saatzucht AG formerly Rabbethge and Giesecke Einbeck, Germany.
authors.forename: Maik
authors.initials: M
authors.lastname: Klie
authors.affiliation: Department of Molecular Plant Breeding, Institute for Plant Genetics, Leibniz Universität Hannover Hannover, Germany ; Saaten-Union Biotec GmbH Leopoldshöhe, Germany.
authors.forename: Stephan
authors.initials: S
authors.lastname: Schie
authors.affiliation: Department of Molecular Plant Breeding, Institute for Plant Genetics, Leibniz Universität Hannover Hannover, Germany.
authors.forename: Marcus
authors.initials: M
authors.lastname: Linde
authors.affiliation: Department of Molecular Plant Breeding, Institute for Plant Genetics, Leibniz Universität Hannover Hannover, Germany.
authors.forename: Thomas
authors.initials: T
authors.lastname: Debener
keywords: allopolyploidy; autopolyploidy; linkage in repulsion; molecular marker; polysomic inheritance; single dose markers