title: Liquid phase based separation systems for depletion, prefractionation, and enrichment of proteins in biological fluids and matrices for in-depth proteomics analysis-An update covering the period 2011-2014.
abstract: This review article expands on the previous one (S. Selvaraju and Z. El Rassi, Electrophoresis 2012, 33, 74-88) by reviewing pertinent literature in the period extending from early 2011 to present. As the previous review article, the present one is concerned with proteomic sample preparation (e.g., depletion of high-abundance proteins, reduction of the protein dynamic concentration range, enrichment of a particular subproteome), and the subsequent chromatographic and/or electrophoretic prefractionation prior to peptide separation and identification by LC-MS/MS. This review article, however, is distinguished from its earlier version by expanding on capturing/enriching subphosphoproteomes by immobilized metal affinity chromatography and metal oxide affinity chromatography. Seventy-seven papers published in the period extending from mid-2011 to the present have been reviewed. By no means this review article is exhaustive, given the fact that its aim is to give a concise treatment of the latest developments in the field.
authors.affiliation: Department of Chemistry, Oklahoma State University, Stillwater, OK, USA.
authors.forename: Chanida
authors.initials: C
authors.lastname: Puangpila
authors.forename: Erandi
authors.initials: E
authors.lastname: Mayadunne
authors.forename: Ziad
authors.initials: Z
authors.lastname: El Rassi
mesh_terms: D000818:Animals; D005591:Chemical Fractionation / Q000295:instrumentation / Q000379:methods*; D002846:Chromatography, Affinity / Q000295:instrumentation / Q000379:methods*; D004586:Electrophoresis / Q000295:instrumentation / Q000379:methods*; D004867:Equipment Design; D006801:Humans; D011506:Proteins / Q000032:analysis / Q000302:isolation & purification*; D040901:Proteomics / Q000295:instrumentation / Q000379:methods*
chemical_list: D011506:Proteins
keywords: Affinity chromatography; Immunodepletion; Protein fractionation; ProteoMiner; Proteomics