title: Maize (Zea mays L.).
abstract: Agrobacterium tumefaciens-mediated transformation is an effective method for introducing genes into maize. In this chapter, we describe a detailed protocol for genetic transformation of the maize genotype Hi II. Our starting plant material is immature embryos cocultivated with an Agrobacterium strain carrying a standard binary vector. In addition to step-by-step laboratory transformation procedures, we include extensive details in growing donor plants and caring for transgenic plants in the greenhouse.
authors.affiliation: Center for Plant Transformation, Plant Sciences Institute, and Department of Agronomy, Iowa State University, 2104 Agronomy Hall, Ames, IA, 50011-1010, USA, bframe@iastate.edu.
authors.forename: Bronwyn
authors.initials: B
authors.lastname: Frame
authors.forename: Katey
authors.initials: K
authors.lastname: Warnberg
authors.forename: Marcy
authors.initials: M
authors.lastname: Main
authors.forename: Kan
authors.initials: K
authors.lastname: Wang
mesh_terms: D000064:Acclimatization; D000383:Agriculture / Q000295:instrumentation / Q000379:methods; D016960:Agrobacterium tumefaciens / Q000235:genetics; D005821:Genetic Techniques; D005822:Genetic Vectors; D030821:Plants, Genetically Modified; D012639:Seeds / Q000235:genetics / Q000254:growth & development; D014170:Transformation, Genetic; D003313:Zea mays / Q000235:genetics* / Q000254:growth & development