title: ETV6 (TEL1) regulates embryonic hematopoiesis in zebrafish.
abstract: Chromosomal translocations involving fusions of the human ETV6 (TEL1) gene occur frequently in hematologic malignancies. However, a detailed understanding of the normal function of ETV6 remains incomplete. This study has employed zebrafish as a relevant model to investigate the role of ETV6 during embryonic hematopoiesis. Zebrafish possessed a single conserved etv6 ortholog that was expressed from 12 hpf in the lateral plate mesoderm, and later in hematopoietic, vascular and other tissues. Morpholino-mediated gene knockdown of etv6 revealed the complex contribution of this gene toward embryonic hematopoiesis. During primitive hematopoiesis, etv6 knockdown resulted in reduced levels of progenitor cells, erythrocyte and macrophage populations, but increased numbers of incompletely differentiated heterophils. Definitive hematopoiesis was also perturbed, with etv6 knockdown leading to decreased erythrocytes and myeloid cells, but enhanced lymphopoiesis. This study suggests that ETV6 plays a broader and more complex role in early hematopoiesis than previously thought, impacting on the development of multiple lineages.
authors.affiliation: School of Medicine, and Strategic Research Centre in Molecular and Medical Research, Deakin University, Geelong.
authors.forename: Parisa
authors.initials: P
authors.lastname: Rasighaemi
authors.affiliation: School of Life & Environmental Sciences, Deakin University, Burwood, Victoria, Australia.
authors.forename: Sara M N
authors.initials: SM
authors.lastname: Onnebo
authors.affiliation: School of Medicine, and Strategic Research Centre in Molecular and Medical Research, Deakin University, Geelong.
authors.forename: Clifford
authors.initials: C
authors.lastname: Liongue
authors.affiliation: School of Medicine, and Strategic Research Centre in Molecular and Medical Research, Deakin University, Geelong award@deakin.edu.au.
authors.forename: Alister C
authors.initials: AC
authors.lastname: Ward
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D019070:Cell Lineage; D002478:Cells, Cultured; D004625:Embryo, Nonmammalian / Q000166:cytology* / Q000378:metabolism; D047108:Embryonic Development; D004912:Erythrocytes / Q000166:cytology / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D006410:Hematopoiesis / Q000502:physiology*; D006801:Humans; D007124:Immunoenzyme Techniques; D008264:Macrophages / Q000166:cytology / Q000378:metabolism; D008648:Mesoderm / Q000166:cytology / Q000378:metabolism; D060172:Morpholinos / Q000494:pharmacology; D022423:Myeloid Cells / Q000166:cytology / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015027:Zebrafish / Q000196:embryology* / Q000378:metabolism; D029961:Zebrafish Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*
chemical_list: D060172:Morpholinos; D012333:RNA, Messenger; D029961:Zebrafish Proteins