title: Virus-induced gene silencing of N gene in tobacco by apple latent spherical virus vectors.
abstract: Virus infections induce an RNA-mediated defense that targets viral RNAs in a nucleotide sequence-specific manner in plants, commonly referred to as virus-induced gene silencing (VIGS). When the virus carries sequences of plant genes, it triggers virus-induced gene silencing (VIGS) and results in the degradation of mRNA of endogenous homologous gene. VIGS has been shown to have great potential as a reverse-genetics tool for studying of gene functions in plants, and it has several advantages over other functional genomics approaches. Here, we describe VIGS of N gene in tobacco cv. Xanthi nc by ALSV vectors containing fragments of N gene from Nicotiana glutinosa.
authors.affiliation: Plant Pathology Laboratory, Faculty of Agriculture, Iwate University, Ueda 3-18-8, Morioka, 020-8550, Japan.
authors.forename: Chunjiang
authors.initials: C
authors.lastname: Li
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Yoshikawa
mesh_terms: D003001:Cloning, Molecular / Q000379:methods; D020868:Gene Silencing; D017343:Genes, Plant; D005822:Genetic Vectors; D008967:Molecular Biology / Q000295:instrumentation / Q000379:methods*; D018515:Plant Leaves / Q000821:virology; D010942:Plant Viruses / Q000235:genetics / Q000472:pathogenicity; D014026:Nicotiana / Q000235:genetics* / Q000254:growth & development / Q000821:virology*