title: Nine novel angiotensin I-converting enzyme (ACE) inhibitory peptides from cuttlefish (Sepia officinalis) muscle protein hydrolysates and antihypertensive effect of the potent active peptide in spontaneously hypertensive rats.
abstract: This study aimed to identify novel ACE inhibitory peptides from the muscle of cuttlefish. Proteins were hydrolyzed and the hydrolysates were then subjected to various types of chromatography to isolate the active peptides. Nine ACE inhibitory peptides were isolated and their molecular masses and amino acid sequences were determined using ESI-MS and ESI-MS/MS, respectively. The structures of the most potent peptides were identified as Val-Glu-Leu-Tyr-Pro, Ala-Phe-Val-Gly-Tyr-Val-Leu-Pro and Glu-Lys-Ser-Tyr-Glu-Leu-Pro. The first peptide displayed the highest ACE inhibitory activity with an IC50 of 5.22μM. Lineweaver-Burk plots suggest that Val-Glu-Leu-Tyr-Pro acts as a non-competitive inhibitor against ACE. Furthermore, antihypertensive effects in spontaneously hypertensive rats (SHR) also revealed that oral administration of Val-Glu-Leu-Tyr-Pro can decrease systolic blood pressure significantly (p<0.01). These results suggest that the Val-Glu-Leu-Tyr-Pro would be a beneficial ingredient for nutraceuticals and pharmaceuticals acting against hypertension and its related diseases.
authors.affiliation: Laboratoire de Procédés Biologiques, Génie Enzymatique et Microbien (ProBioGEM), UPRES-EA 1026, Polytech'Lille/IUT «A», Université Lille I Sciences et Technologies, BP 179, 59655 Villeneuve d'Ascq Cedex, France. Electronic address: rafikbalti1981@gmail.com.
authors.forename: Rafik
authors.initials: R
authors.lastname: Balti
authors.affiliation: Higher Institute of Biotechnology of Sfax, University of Sfax, Km 4 Road Soukra, 3038 Sfax, Tunisia.
authors.forename: Ali
authors.initials: A
authors.lastname: Bougatef
authors.affiliation: Laboratoire de Procédés Biologiques, Génie Enzymatique et Microbien (ProBioGEM), UPRES-EA 1026, Polytech'Lille/IUT «A», Université Lille I Sciences et Technologies, BP 179, 59655 Villeneuve d'Ascq Cedex, France.
authors.forename: Assaâd
authors.initials: A
authors.lastname: Sila
authors.affiliation: Laboratoire de Procédés Biologiques, Génie Enzymatique et Microbien (ProBioGEM), UPRES-EA 1026, Polytech'Lille/IUT «A», Université Lille I Sciences et Technologies, BP 179, 59655 Villeneuve d'Ascq Cedex, France.
authors.forename: Didier
authors.initials: D
authors.lastname: Guillochon
authors.affiliation: Laboratoire de Procédés Biologiques, Génie Enzymatique et Microbien (ProBioGEM), UPRES-EA 1026, Polytech'Lille/IUT «A», Université Lille I Sciences et Technologies, BP 179, 59655 Villeneuve d'Ascq Cedex, France.
authors.forename: Pascal
authors.initials: P
authors.lastname: Dhulster
authors.affiliation: Laboratoire de Procédés Biologiques, Génie Enzymatique et Microbien (ProBioGEM), UPRES-EA 1026, Polytech'Lille/IUT «A», Université Lille I Sciences et Technologies, BP 179, 59655 Villeneuve d'Ascq Cedex, France.
authors.forename: Naima
authors.initials: N
authors.lastname: Nedjar-Arroume
mesh_terms: D000595:Amino Acid Sequence; D000806:Angiotensin-Converting Enzyme Inhibitors / Q000032:analysis / Q000302:isolation & purification / Q000494:pharmacology; D000818:Animals; D000959:Antihypertensive Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D001794:Blood Pressure / Q000187:drug effects*; D002850:Chromatography, Gel; D049832:Decapodiformes / Q000378:metabolism*; D006973:Hypertension / Q000473:pathology; D008297:Male; D008970:Molecular Weight; D009132:Muscles / Q000737:chemistry* / Q000201:enzymology; D010455:Peptides / Q000032:analysis / Q000302:isolation & purification / Q000494:pharmacology; D011492:Protein Hydrolysates / Q000737:chemistry; D055550:Protein Stability; D051381:Rats; D011918:Rats, Inbred SHR; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D000806:Angiotensin-Converting Enzyme Inhibitors; D000959:Antihypertensive Agents; D010455:Peptides; D011492:Protein Hydrolysates
keywords: ACE inhibitory peptide; Antihypertensive effect; Cuttlefish muscle protein; Enzymatic hydrolysate; Purification and identification