title: Long noncoding RNA expression in dermal papilla cells contributes to hairy gene regulation.
abstract: Dermal papilla (DP) cells may be the source of dermal-derived signaling molecules involved in hair-follicle development and postnatal hair cycling. Early-passage DP cells can induce hair growth in vivo, but, on further culture, this ability is lost. The cellular mechanisms underlying the hair-follicle induction property of early-passage DP cells are unclear. Long noncoding RNAs (lncRNAs) are an important class of genes involved in various biological functions. They are aberrantly expressed and play roles in the regulation of the Wnt signaling pathway, a critical point in maintaining hair-induction activity. LncRNA microarray revealed 1683 upregulated and 1773 downregulated lncRNAs in passage-4 DP cells compare with passage-10 DP cells. To investigate the relation between lncRNAs and coding genes in WNT signaling, we constructed a coding-noncoding gene co-expression network using lncRNAs and coding genes that were differentially expressed between the passage-4 and -10 DP cells. RP11-766N7.3, H19 and HOTAIR are specific lncRNAs that were aberrantly expressed in DP cells and played an important role in regulating Wnt signaling. This study may provide potential targets for discovering the hair-follicle induction mechanism of early-passage DP cells.
authors.affiliation: Department of Histology and Embryology, Shantou University Medical College, Xinling No. 22, Shantou 515041, China.
authors.forename: Chang-Min
authors.initials: CM
authors.lastname: Lin
authors.affiliation: Department of Histology and Embryology, Shantou University Medical College, Xinling No. 22, Shantou 515041, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Emergency, Second Affiliated Hospital, Shantou University Medical College, Shantou, China.
authors.forename: Keng
authors.initials: K
authors.lastname: Huang
authors.affiliation: Department of Histology and Embryology, Shantou University Medical College, Xinling No. 22, Shantou 515041, China.
authors.forename: Xian-Cai
authors.initials: XC
authors.lastname: Chen
authors.affiliation: Tissue Engineering Laboratory, First Affiliated Hospital, Shantou University Medical College, Changping No. 57, Shantou 515041, China.
authors.forename: Bo-Zhi
authors.initials: BZ
authors.lastname: Cai
authors.affiliation: Department of Emergency, Second Affiliated Hospital, Shantou University Medical College, Shantou, China.
authors.forename: Hai-Hong
authors.initials: HH
authors.lastname: Li
authors.affiliation: Department of Histology and Embryology, Shantou University Medical College, Xinling No. 22, Shantou 515041, China.
authors.forename: Yan-Ping
authors.initials: YP
authors.lastname: Yuan
authors.affiliation: Department of Histology and Embryology, Shantou University Medical College, Xinling No. 22, Shantou 515041, China.
authors.forename: Huan
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Tissue Engineering Laboratory, First Affiliated Hospital, Shantou University Medical College, Changping No. 57, Shantou 515041, China. Electronic address: yli@stu.edu.cn.
authors.forename: Yu
authors.initials: Y
authors.lastname: Li
mesh_terms: D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D020869:Gene Expression Profiling; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D020411:Oligonucleotide Array Sequence Analysis; D062085:RNA, Long Noncoding / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012867:Skin / Q000166:cytology / Q000378:metabolism*
chemical_list: D017931:DNA Primers; D062085:RNA, Long Noncoding
keywords: Dermal papilla cells; Hair follicle; Long noncoding RNAs; Wnt