title: Comparison of the Antibacterial Efficacy of Manuka Honey Against E.faecalis and E.coli - An In vitro Study.
abstract: OBJECTIVE
  To compare the antibacterial efficacy of Manuka honey against E.faecalis and E.coli.
  
  
  METHODS
  Escherichia coli (ATCC-25922) and Enterococcus faecalis (ATCC-29212) were separately inoculated in the nutrient broth and incubated at 37(o)C for 24-48 hrs. Bacterial samples were kept in contact with each disinfectant solution for varying intervals of time. Once the test time had elapsed 10μL of the bacterial dilutions were plated on Mueller-Hinton agar and incubated for 24-48 hrs at 37°C to estimate the density. Study of the disinfection process with respect to time and Modeling was done.
  
  
  RESULTS
  The mean value of the antimicrobial activity of Manuka honey against E.coli and E.faecalis are 1.55 and 0.36 respectively and are relatively higher. It shows that there is a significant difference among the various root canal disinfectant groups against E. coli and E. feacalis. (p<0.001) CONCLUSION: Manuka honey is shown to be a potential root canal disinfectant against gram positive and gram negative bacterial pathogens.
authors.affiliation: Senior Lecturer, Department of Conservative Dentistry and Endodontics, RVS College of Dental Sciences , Coimbatore, Tamil Nadu, India .
authors.forename: N Deepak
authors.initials: ND
authors.lastname: Kumar
authors.affiliation: Senior Lecturer, Department of Conservative Dentistry and Endodontics, Sibar Institute of Dental Sciences , Guntur, Andhra Pradesh, India .
authors.forename: Rama S
authors.initials: RS
authors.lastname: Kalluru
authors.affiliation: Professor, Department of Conservative Dentistry and Endodontics, Raja Muthaiah Dental College , Chidambaram, Tamil Nadu, India .
authors.forename: Shafie
authors.initials: S
authors.lastname: Ahmed
authors.affiliation: Senior Lecturer, Department of Pedodontics, RVS College of Dental Sciences , Coimbatore, Tamil Nadu, India .
authors.forename: A
authors.initials: A
authors.lastname: Abhilashini
authors.affiliation: Professor, Department of Conservative Dentistry and Endodontics, St.Joseph Dental College , Eluru, Andhra Pradesh, India .
authors.forename: Thumu
authors.initials: T
authors.lastname: Jayaprakash
authors.affiliation: Senior Lecturer, Department of Conservative Dentistry and Endodontics, Kamineni Institute of Dental Sciences , Narketpally, Andhra Pradesh, India .
authors.forename: Roopadevi
authors.initials: R
authors.lastname: Garlapati
authors.affiliation: Lecturer, Department of Pedodontics, at Sibar Institute of Dental Sciences , Guntur, Andhra Pradesh, India .
authors.forename: Butti
authors.initials: B
authors.lastname: Sowmya
authors.affiliation: Reader, Department of Conservative Dentistry and Endodontics, Sibar Institute of Dental Sciences , Guntur, India .
authors.forename: K Narasimha
authors.initials: KN
authors.lastname: Reddy
keywords: Antibacterial efficacy; Death kinetics; Manuka honey