title: Visceral mesh modified with cyclodextrin for the local sustained delivery of ropivacaine.
abstract: The aim of the study was to develop a polyester visceral implant modified with a cyclodextrin polymer for the local and prolonged delivery of ropivacaine to reduce post operatory pain. Therefore, we applied a coating of an inguinal mesh with a crosslinked polymer of hydroxypropyl-β-cyclodextrin (HPβCD) whose specific host-guest complex forming properties were expected to improve the adsorption capacity of the implant toward anesthetic, and then to release it within a sustained period. The modification reaction of the textile with cyclodextrin was explored through the study of the influence of the pad/dry/cure process parameters and the resulting implant (PET-CD) was characterized by solid state NMR and SEM. Besides, the inclusion complex between ropivacaine and CD was studied by NMR and capillary electrophoresis in PBS medium. Finally, ropivacaine sorption test showed that a maximum of 30 mg/g of ropivacaine could be adsorbed on the functionalized samples. In dynamic batch tests in PBS at pH 7.4, the release could be observed up to 6h. The cytocompatibility of the PET-CD loaded with ropivacaine was also studied and reached 65% cell vitality after 6 days.
authors.affiliation: Unité Matériaux Et Transformations (UMET), Université Lille 1, 59655 Villeneuve d'Ascq, France; Cousin Biotech, 59117 Wervicq-Sud, France.
authors.forename: G
authors.initials: G
authors.lastname: Vermet
authors.affiliation: Unité Matériaux Et Transformations (UMET), Université Lille 1, 59655 Villeneuve d'Ascq, France. Electronic address: stephanie.degoutin@univ-lille1.fr.
authors.forename: S
authors.initials: S
authors.lastname: Degoutin
authors.affiliation: INSERM U 1008, Controlled Drug Delivery Systems and Biomaterials, Université Lille 2, 59006 Lille, France.
authors.forename: F
authors.initials: F
authors.lastname: Chai
authors.affiliation: INSERM U 1008, Controlled Drug Delivery Systems and Biomaterials, Université Lille 2, 59006 Lille, France.
authors.forename: M
authors.initials: M
authors.lastname: Maton
authors.affiliation: Centre Commun de Mesures RMN, Université Lille 1, 59655 Villeneuve d'Ascq, France.
authors.forename: M
authors.initials: M
authors.lastname: Bria
authors.affiliation: UDSL, Laboratoire de Chimie Analytique EA 4481, Université Lille 2, 59000 Lille, France.
authors.forename: C
authors.initials: C
authors.lastname: Danel
authors.affiliation: INSERM U 1008, Controlled Drug Delivery Systems and Biomaterials, Université Lille 2, 59006 Lille, France.
authors.forename: H F
authors.initials: HF
authors.lastname: Hildebrand
authors.affiliation: INSERM U 1008, Controlled Drug Delivery Systems and Biomaterials, Université Lille 2, 59006 Lille, France.
authors.forename: N
authors.initials: N
authors.lastname: Blanchemain
authors.affiliation: Unité Matériaux Et Transformations (UMET), Université Lille 1, 59655 Villeneuve d'Ascq, France.
authors.forename: B
authors.initials: B
authors.lastname: Martel
mesh_terms: D000073738:2-Hydroxypropyl-beta-cyclodextrin; D000327:Adsorption; D000577:Amides / Q000008:administration & dosage* / Q000737:chemistry; D000779:Anesthetics, Local / Q000008:administration & dosage* / Q000737:chemistry; D000818:Animals; D002478:Cells, Cultured; D003692:Delayed-Action Preparations; D016503:Drug Delivery Systems; D004343:Drug Implants; D005079:Excipients / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D041681:NIH 3T3 Cells; D010149:Postoperative Pain / Q000517:prevention & control; D011091:Polyesters / Q000737:chemistry; D000077212:Ropivacaine; D013997:Time Factors; D047392:beta-Cyclodextrins / Q000737:chemistry*
chemical_list: D000577:Amides; D000779:Anesthetics, Local; D003692:Delayed-Action Preparations; D004343:Drug Implants; D005079:Excipients; D011091:Polyesters; D047392:beta-Cyclodextrins; D000073738:2-Hydroxypropyl-beta-cyclodextrin; D000077212:Ropivacaine
keywords: Cyclodextrin; Local anesthetic; Prolonged delivery; Ropivacaine; Surface modification; Visceral mesh textile