title: A model for the coordinated stepping of cytoplasmic dynein.
abstract: Cytoplasmic dynein play an important role in transporting various intracellular cargos by coupling their ATP hydrolysis cycle with their conformational changes. Recent experimental results showed that the cytoplasmic dynein had a highly variable stepping pattern including "hand-over-hand", "inchworm" and "nonalternating-inchworm". Here, we developed a model to describe the coordinated stepping patterns of cytoplasmic dynein, based on its working cycle, construction and the interaction between its leading head and tailing head. The kinetic model showed how change in the distance between the two heads influences the rate of cytoplasmic dynein under different stepping patterns. Numerical simulations of the distribution of step size and striding rate are in good quantitative agreement with experimental observations. Hence, our coordinated stepping model for cytoplasmic dynein successfully explained its diverse stepping patterns as a molecular motor. The cooperative mechanism carried out by the two heads of cytoplasmic dynein shed light on the strategies adopted by the cytoplasmic dynein in executing various functions.
authors.affiliation: School of Physical Science and Technology, Inner Mongolia University, 235 West Street, 010021 Hohhot, Inner Mongolia, China; Inner Mongolia Vocational College of Chemical Engineering, Higher Vocational Technology Park, 010070 Hohhot, Inner Mongolia, China.
authors.forename: X Y
authors.initials: XY
authors.lastname: Zhao
authors.affiliation: School of Physical Science and Technology, Inner Mongolia University, 235 West Street, 010021 Hohhot, Inner Mongolia, China; Department of Information and Automation, Ordos Vocational College, Yikezhao Street, Kangbashi New District, 017000 Ordos, Inner Mongolia, China.
authors.forename: W
authors.initials: W
authors.lastname: Sun
authors.affiliation: School of Physical Science and Technology, Inner Mongolia University, 235 West Street, 010021 Hohhot, Inner Mongolia, China.
authors.forename: J P
authors.initials: JP
authors.lastname: Zhang
authors.affiliation: School of Physical Science and Technology, Inner Mongolia University, 235 West Street, 010021 Hohhot, Inner Mongolia, China.
authors.lastname: Tala
authors.affiliation: School of Physical Science and Technology, Inner Mongolia University, 235 West Street, 010021 Hohhot, Inner Mongolia, China. Electronic address: pygws@imu.edu.cn.
authors.forename: W S
authors.initials: WS
authors.lastname: Guo
mesh_terms: D000255:Adenosine Triphosphate / Q000737:chemistry* / Q000378:metabolism*; D000818:Animals; D001665:Binding Sites; D001693:Biological Transport, Active / Q000502:physiology; D003198:Computer Simulation; D056445:Cytoplasmic Dyneins / Q000737:chemistry* / Q000378:metabolism* / Q000648:ultrastructure; D004735:Energy Transfer / Q000502:physiology; D006801:Humans; D006868:Hydrolysis; D040542:Mechanotransduction, Cellular / Q000502:physiology*; D008954:Models, Biological; D008956:Models, Chemical; D009038:Motion; D011485:Protein Binding
chemical_list: D000255:Adenosine Triphosphate; D056445:Cytoplasmic Dyneins
keywords: Cytoplasmic dynein complex; Elastic linkage; Mechanochemical cycle; Step size distribution; Striding rate