title: Gadolinium-chelate nanoparticle entrapped human mesenchymal stem cell via photochemical internalization for cancer diagnosis.
abstract: To improve the gadolinium (Gd) internalization efficiency in stem cells, gadolinium-chelate nanoparticles were prepared from a pullulan derivative (pullulan-deoxycholic acid (DOCA)-diethylene triamine pentaacetic (DTPA)-Gd conjugate; PDDG) and then the PDDG was entrapped into human mesenchymal stem cells (hMSCs) by the photochemical-internalization (PCI) method for cancer diagnosis via the cancer homing property of hMSCs. The internalization efficiency of Gd in hMSCs was significantly increased to 98 ± 4 pg Gd/cell from 32 ± 2 pg Gd/cell via the PCI method. Moreover, the Gd-entrapped hMSCs revealed a low exocytosis ratio of gadolinium-chelate nanoparticles during cell division in vitro and a high cellular labeling efficiency for at least 21 days in vivo. The cancer-targeting and diagnosis effect of the Gd-entrapped hMSCs were confirmed in a small CT26 tumor-bearing mice model. The stem cells detected an early tumor (∼3 mm(3)) within 2 h using 4.7-T MR and optical imaging. The results demonstrated that the PCI-mediated internalization of Gd-incorporated nanoparticles into hMSCs is a promising protocol for efficient cell labeling and tracking.
authors.affiliation: Department of Biotechnology, The Catholic University of Korea, 43 Jibong ro, Wonmi-gu, Bucheon-si, Gyeonggi do 420-743, Republic of Korea.
authors.forename: Kyoung Sub
authors.initials: KS
authors.lastname: Kim
authors.affiliation: Department of Biotechnology, The Catholic University of Korea, 43 Jibong ro, Wonmi-gu, Bucheon-si, Gyeonggi do 420-743, Republic of Korea.
authors.forename: Wooram
authors.initials: W
authors.lastname: Park
authors.affiliation: Department of Biotechnology, The Catholic University of Korea, 43 Jibong ro, Wonmi-gu, Bucheon-si, Gyeonggi do 420-743, Republic of Korea. Electronic address: kna6997@catholic.ac.kr.
authors.forename: Kun
authors.initials: K
authors.lastname: Na
mesh_terms: D000818:Animals; D002460:Cell Line; D002614:Chelating Agents / Q000008:administration & dosage / Q000737:chemistry; D003287:Contrast Media / Q000008:administration & dosage* / Q000737:chemistry; D005260:Female; D019786:Gadolinium DTPA / Q000008:administration & dosage* / Q000737:chemistry; D006801:Humans; D008279:Magnetic Resonance Imaging; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D051379:Mice; D008807:Mice, Inbred BALB C; D053758:Nanoparticles / Q000008:administration & dosage / Q000737:chemistry; D009369:Neoplasms / Q000175:diagnosis*; D061848:Optical Imaging
chemical_list: D002614:Chelating Agents; D003287:Contrast Media; D019786:Gadolinium DTPA
keywords: Cancer diagnosis; Human mesenchymal stem cell (hMSC); Stem cell engineering; Stem cell homing; Tumor-targeted delivery