title: Impact of enzymatic pretreatment on corn stover degradation and biogas production.
abstract: Corn stover is an agricultural residue consisting of lignocellulose, cellulose and hemicellulose polymers, sheeted in a lignin barrier. Corn stover can be used as feedstock for biogas production. Previous studies have shown biological pretreatment of lignocellulose materials can increase digestibility of the substrate improving hydrolysis, the rate-limiting step in biogas production. The impact of pretreating with different enzymes (laccase, manganese peroxidase and versatile peroxidase) and different incubation times, (0, 6 and 24 h) was studied. The effect on the matrix and biomethane production was determined. Pretreatments did not yield high concentrations of phenolic compounds, inhibitors of biogas production. The laccase enzyme showed an increase in biomethane production of 25% after 24 h of incubation. Pretreatment with peroxidase enzymes increased biomethane production with 17% after 6 h of incubation. As such it can be concluded that by introducing the different enzymes at different stages during pretreatment an increased biomethane production can be obtained.
authors.affiliation: Department of Industrial Biological Sciences, Faculty of Bioscience Engineering, Ghent University, Campus Kortrijk, Belgium.
authors.forename: Michel
authors.initials: M
authors.lastname: Schroyen
authors.affiliation: Department of Industrial Biological Sciences, Faculty of Bioscience Engineering, Ghent University, Campus Kortrijk, Belgium.
authors.forename: Han
authors.initials: H
authors.lastname: Vervaeren
authors.affiliation: Department of Industrial Biological Sciences, Faculty of Bioscience Engineering, Ghent University, Campus Kortrijk, Belgium.
authors.forename: Stijn W H
authors.initials: SWH
authors.lastname: Van Hulle
authors.affiliation: Department of Industrial Biological Sciences, Faculty of Bioscience Engineering, Ghent University, Campus Kortrijk, Belgium. Electronic address: Katleen.Raes@UGent.be.
authors.forename: Katleen
authors.initials: K
authors.lastname: Raes
mesh_terms: D000383:Agriculture / Q000379:methods; D001673:Biodegradation, Environmental; D007220:Industrial Waste / Q000517:prevention & control*; D042845:Laccase / Q000737:chemistry*; D008697:Methane / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D035261:Plant Components, Aerial / Q000737:chemistry* / Q000382:microbiology*; D003313:Zea mays / Q000737:chemistry* / Q000382:microbiology*
chemical_list: D007220:Industrial Waste; D042845:Laccase; D008697:Methane
keywords: Biological pretreatment; Biomethane; Corn stover; Enzymes; Phenolic compounds