title: Long-chain bases and their phosphorylated derivatives differentially regulate cryptogein-induced production of reactive oxygen species in tobacco (Nicotiana tabacum) BY-2 cells.
abstract: The proteinaceous elicitor cryptogein triggers defence reactions in Nicotiana tabacum (tobacco) through a signalling cascade, including the early production of reactive oxygen species (ROS) by the plasma membrane (PM)-located tobacco respiratory burst oxidase homologue D (NtRbohD). Sphingolipid long-chain bases (LCBs) are emerging as potent positive regulators of plant defence-related mechanisms. This led us to question whether both LCBs and their phosphorylated derivatives (LCB-Ps) are involved in the early signalling process triggered by cryptogein in tobacco BY-2 cells. Here, we showed that cryptogein-induced ROS production was inhibited by LCB kinase (LCBK) inhibitors. Additionally, Arabidopsis thaliana sphingosine kinase 1 and exogenously supplied LCB-Ps increased cryptogein-induced ROS production, whereas exogenously supplied LCBs had a strong opposite effect, which was not driven by a reduction in cellular viability. Immunogold-electron microscopy assay also revealed that LCB-Ps are present in the PM, which fits well with the presence of a high LCBK activity associated with this fraction. Our data demonstrate that LCBs and LCB-Ps differentially regulate cryptogein-induced ROS production in tobacco BY-2 cells, and support a model in which a cooperative synergism between LCBK/LCB-Ps and NtRbohD/ROS in the cryptogein signalling pathway is likely at the PM in tobacco BY-2 cells.
authors.affiliation: INRA, Institut Jean-Pierre Bourgin, UMR 1318, ERL CNRS 3559, Saclay Plant Sciences, RD10, F-78026, Versailles, France.|AgroParisTech, Institut Jean-Pierre Bourgin, UMR 1318, ERL CNRS 3559, Saclay Plant Sciences, RD10, F-78026, Versailles, France.
authors.forename: Sylvie
authors.identifier: http://orcid.org/0000-0001-8689-310X
authors.initials: S
authors.lastname: Coursol
authors.affiliation: INRA, UMR 1347 Agroécologie, ERL CNRS 6300, BP 86510, F-21065, Dijon Cedex, France.
authors.forename: Jérôme
authors.initials: J
authors.lastname: Fromentin
authors.affiliation: INRA, UMR 1347 Agroécologie, ERL CNRS 6300, BP 86510, F-21065, Dijon Cedex, France.
authors.forename: Elodie
authors.initials: E
authors.lastname: Noirot
authors.affiliation: Laboratoire de Recherche en Sciences Végétales, UMR 5546, Université de Toulouse, BP 42617, F-31326, Castanet-Tolosan, France.|Laboratoire de Recherche en Sciences Végétales, CNRS, UMR 5546, BP 42617, F-31326, Castanet-Tolosan, France.
authors.forename: Christian
authors.initials: C
authors.lastname: Brière
authors.affiliation: INRA, UMR 1347 Agroécologie, ERL CNRS 6300, BP 86510, F-21065, Dijon Cedex, France.
authors.forename: Franck
authors.initials: F
authors.lastname: Robert
authors.affiliation: INRA, UMR 1347 Agroécologie, ERL CNRS 6300, BP 86510, F-21065, Dijon Cedex, France.
authors.forename: Johanne
authors.initials: J
authors.lastname: Morel
authors.affiliation: State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan, 430072, China.
authors.forename: Yun-Kuan
authors.initials: YK
authors.lastname: Liang
authors.affiliation: INRA, UMR 1347 Agroécologie, ERL CNRS 6300, BP 86510, F-21065, Dijon Cedex, France.
authors.forename: Jeannine
authors.initials: J
authors.lastname: Lherminier
authors.affiliation: INRA, UMR 1347 Agroécologie, ERL CNRS 6300, BP 86510, F-21065, Dijon Cedex, France.
authors.forename: Françoise
authors.initials: F
authors.lastname: Simon-Plas
mesh_terms: D016923:Cell Death / Q000187:drug effects; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D005656:Fungal Proteins / Q000494:pharmacology*; D010766:Phosphorylation / Q000187:drug effects; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000037:antagonists & inhibitors / Q000378:metabolism; D059828:Plant Cells / Q000187:drug effects / Q000378:metabolism; D047428:Protein Kinase Inhibitors / Q000494:pharmacology; D021381:Protein Transport / Q000187:drug effects; D017382:Reactive Oxygen Species / Q000378:metabolism*; D013107:Sphingolipids / Q000378:metabolism*; D013347:Subcellular Fractions / Q000187:drug effects / Q000378:metabolism; D014026:Nicotiana / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*
chemical_list: D005656:Fungal Proteins; D047428:Protein Kinase Inhibitors; D017382:Reactive Oxygen Species; D013107:Sphingolipids; C061032:cryptogein protein, Phytophthora cryptogea; D017853:Phosphotransferases (Alcohol Group Acceptor); C426837:sphingolipid long-chain base kinase
keywords: Nicotiana tabacum (tobacco) BY-2 cells; Nicotiana tabacum respiratory burst oxidase homologue D (NtRbohD); cryptogein elicitor; long-chain base (LCB); long-chain base kinase; plasma membrane; reactive oxygen species (ROS)