title: Luteolin inhibited the gene expression, production and secretion of MUC5AC mucin via regulation of nuclear factor kappa B signaling pathway in human airway epithelial cells.
abstract: Luteolin, a flavonoidal compound derived from Lonicera japonica Thunb. and Chrysanthemum indicum L., has been reported to show anti-inflammatory, anti-oxidative and anti-carcinogenic effects. In this study, we investigated whether luteolin significantly affects the secretion, production and gene expression of airway mucin. Confluent NCI-H292 cells were pretreated with luteolin for 30 min and then stimulated with EGF (epidermal growth factor) or PMA (phorbol 12-myristate 13-acetate) for 24 h or the indicated periods. The MUC5AC mucin gene expression was measured by RT-PCR. Production and secretion of MUC5AC mucin protein were measured by ELISA. To elucidate the action mechanism of luteolin, effect of luteolin on PMA-induced NF-κB signaling pathway was investigated by western blot analysis. The results were as follows: (1) Luteolin inhibited the secretion of MUC5AC mucin protein induced by EGF or PMA; (2) Luteolin inhibited the production of MUC5AC mucin protein and the expression of MUC5AC mucin gene induced by EGF or PMA; (3) Luteolin inhibited PMA-induced phosphorylation and degradation of inhibitory kappa Bα (IκBα); (4) Luteolin inhibited PMA-induced phosphorylation and nuclear translocation of nuclear factor kappa B (NF-κB) p65. This result suggests that luteolin can regulate the secretion, production and gene expression of mucin by acting on airway epithelial cells via regulation of NF-kB signaling pathway.
authors.affiliation: Department of Pharmacology, School of Medicine, Chungnam National University, Daejeon, Republic of Korea.
authors.forename: Hyun Jae
authors.initials: HJ
authors.lastname: Lee
authors.affiliation: Department of Pharmacology, School of Medicine, Chungnam National University, Daejeon, Republic of Korea.
authors.forename: Hyo-Seok
authors.initials: HS
authors.lastname: Seo
authors.affiliation: Department of Pharmacology, School of Medicine, Chungnam National University, Daejeon, Republic of Korea.
authors.forename: Jiho
authors.initials: J
authors.lastname: Ryu
authors.affiliation: Department of Pharmacology, School of Medicine, Chungnam National University, Daejeon, Republic of Korea.
authors.forename: Yong Pill
authors.initials: YP
authors.lastname: Yoon
authors.affiliation: Department of Pharmacology, School of Medicine, Chungnam National University, Daejeon, Republic of Korea.
authors.forename: Su Hyun
authors.initials: SH
authors.lastname: Park
authors.affiliation: Department of Pharmacology, School of Medicine, Chungnam National University, Daejeon, Republic of Korea. Electronic address: LCJ123@cnu.ac.kr.
authors.forename: Choong Jae
authors.initials: CJ
authors.lastname: Lee
mesh_terms: D002460:Cell Line; D004815:Epidermal Growth Factor / Q000494:pharmacology; D004847:Epithelial Cells / Q000378:metabolism; D015870:Gene Expression / Q000187:drug effects*; D006801:Humans; D047311:Luteolin; D055271:Mucin 5AC / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism*; D016328:NF-kappa B / Q000187:drug effects*; D020545:Respiratory Mucosa / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction / Q000187:drug effects*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
chemical_list: D055271:Mucin 5AC; D016328:NF-kappa B; D004815:Epidermal Growth Factor; D047311:Luteolin; D013755:Tetradecanoylphorbol Acetate
keywords: Airway; Luteolin; Mucin