title: Disease resistance of pacu Piaractus mesopotamicus (Holmberg, 1887) fed with β-glucan.
abstract: Effects of β-glucan on innate immune responses and survival were studied in pacu experimentally infected with Aeromonas hydrophila. Fish fed diets containing 0, 0.1% and 1% β-glucan were injected with A. hydrophila. β-glucan enhanced fish survival in both treated groups (26.7% and 21.2% of the control, respectively). Leukocyte respiratory burst and alternative complement pathway activities were elevated after bacterial challenge regardless the β-glucan concentration. Lysozyme activity was higher after infection and showed a gradual increase as β-glucan concentration increased. A significant elevation in WBC count was observed either after bacterial challenge or by influence of β-glucan separately. The same response was observed in the number of thrombocytes, lymphocytes, eosinophils, LG-PAS positive cell and monocytes. It can be concluded that feeding pacu with β-glucan can increase protection against A. hydrophila, due to changes in non-specific immune responses.
authors.affiliation: Faculdade de Ciências Agrárias e Veterinárias, Universidade Estadual Paulista - UNESP, Jaboticabal, SP, Brazil.
authors.forename: J D
authors.initials: JD
authors.lastname: Biller-Takahashi
authors.affiliation: Faculdade de Zootecnia, Universidade Estadual Paulista - UNESP, Dracena, SP, Brazil.
authors.forename: L S
authors.initials: LS
authors.lastname: Takahashi
authors.affiliation: Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo -USP, Ribeirão Preto, SP, Brazil.
authors.forename: C M
authors.initials: CM
authors.lastname: Marzocchi-Machado
authors.affiliation: Centro de Aquicultura da UNESP - CAUNESP, Universidade Estadual Paulista - UNESP, Jaboticabal, SP, Brazil.
authors.forename: F S
authors.initials: FS
authors.lastname: Zanuzzo
authors.affiliation: Faculdade de Ciências Agrárias e Veterinárias, Universidade Estadual Paulista - UNESP, Jaboticabal, SP, Brazil.
authors.forename: E C
authors.initials: EC
authors.lastname: Urbinati
mesh_terms: D016980:Aeromonas hydrophila; D000821:Animal Feed; D000818:Animals; D060467:Disease Resistance / Q000187:drug effects / Q000276:immunology*; D005393:Fish Diseases / Q000276:immunology*; D016905:Gram-Negative Bacterial Infections / Q000276:immunology / Q000662:veterinary*; D016019:Survival Analysis; D047071:beta-Glucans / Q000008:administration & dosage*
chemical_list: D047071:beta-Glucans