title: A new aldehyde oxidase catalyzing the conversion of glycolaldehyde to glycolate from Burkholderia sp. AIU 129.
abstract: We found a new aldehyde oxidase (ALOD), which catalyzes the conversion of glycolaldehyde to glycolate, from Burkholderia sp. AIU 129. The enzyme further oxidized aliphatic aldehydes, an aromatic aldehyde, and glyoxal, but not glycolate or alcohols. The molecular mass of this enzyme was 130 kDa, and it was composed of three different subunits (αβγ structure), in which the α, β, and γ subunits were 76 kDa, 36 kDa, and 14 kDa, respectively. The N-terminal amino acid sequences of each subunit showed high similarity to those of putative subunits of xanthine dehydrogenase. Metals (copper, iron and molybdenum) and chelating reagents (α,α'-dipyridyl and 8-hydroxyquinoline) inhibited the ALOD activity. The ALOD showed highest activity at pH 6.0 and 50°C. Twenty mM glycolaldehyde was completely converted to glycolate by incubation at 30°C for 3 h, suggesting that the ALOD found in this study would be useful for enzymatic production of glycolate.
authors.affiliation: Department of Biological Chemistry and Food Science, Iwate University, Ueda-3, Morioka 020-8550, Japan.
authors.forename: Miwa
authors.initials: M
authors.lastname: Yamada
authors.affiliation: Department of Biological Chemistry and Food Science, Iwate University, Ueda-3, Morioka 020-8550, Japan.
authors.forename: Keika
authors.initials: K
authors.lastname: Adachi
authors.affiliation: Department of Biological Chemistry and Food Science, Iwate University, Ueda-3, Morioka 020-8550, Japan.
authors.forename: Natsumi
authors.initials: N
authors.lastname: Ogawa
authors.affiliation: Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kitashirakawa-oiwakecho, Sakyo-ku, Kyoto 606-8502, Japan.
authors.forename: Shigenobu
authors.initials: S
authors.lastname: Kishino
authors.affiliation: Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kitashirakawa-oiwakecho, Sakyo-ku, Kyoto 606-8502, Japan.
authors.forename: Jun
authors.initials: J
authors.lastname: Ogawa
authors.affiliation: Division of Applied Life Sciences, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, 1-1 Gakuen-cho, Naka-ku, Sakai 599-8531, Japan.
authors.forename: Michihiko
authors.initials: M
authors.lastname: Kataoka
authors.affiliation: Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kitashirakawa-oiwakecho, Sakyo-ku, Kyoto 606-8502, Japan; Faculty of Bioenvironmental Science, Kyoto Gakuen University, Sogabe-cho, Kameoka 621-8555, Japan.
authors.forename: Sakayu
authors.initials: S
authors.lastname: Shimizu
authors.affiliation: Department of Biological Chemistry and Food Science, Iwate University, Ueda-3, Morioka 020-8550, Japan. Electronic address: kiso@iwate-u.ac.jp.
authors.forename: Kimiyasu
authors.initials: K
authors.lastname: Isobe
mesh_terms: D000079:Acetaldehyde / Q000031:analogs & derivatives* / Q000378:metabolism; D042931:Aldehyde Oxidase / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000378:metabolism*; D000447:Aldehydes / Q000378:metabolism; D000595:Amino Acid Sequence; D055162:Biocatalysis; D019117:Burkholderia / Q000201:enzymology*; D006016:Glycolates / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D008670:Metals / Q000494:pharmacology; D008970:Molecular Weight; D010084:Oxidation-Reduction; D021122:Protein Subunits / Q000737:chemistry / Q000378:metabolism; D013696:Temperature; D014968:Xanthine Dehydrogenase / Q000737:chemistry
chemical_list: D000447:Aldehydes; D006016:Glycolates; D008670:Metals; D021122:Protein Subunits; C031149:glycolic acid; D014968:Xanthine Dehydrogenase; D042931:Aldehyde Oxidase; D000079:Acetaldehyde; C010972:glycolaldehyde
keywords: Aldehyde oxidase; Burkholderia sp.; Glycolaldehyde; Glycolic acid; Xanthine oxidase/dehydrogenase