title: Comparison of the interferon-gamma release assay with the traditional methods for detecting Mycobacterium tuberculosis infection in children.
abstract: The purpose of the article is to compare the whole blood interferon-γ release assay (IGRA) with the traditional methods for detecting Mycobacterium tuberculosis (MTB) infection in children. Fifteen childhood patients with tuberculosis and 15 healthy children were recruited. Sputa samples and venous blood were collected, and according to different procedures, IGRA, sputum smear, colloidal gold assay (CGA), fluorescence quantitation polymerase chain reaction (FQ-PCR), and tuberculosis skin test (TST) were, respectively, performed. Thirty healthy children vaccinated with Bacillus Calmette-Guérin (BCG) were also recruited, and the comparative test was carried out between IGRA and TST. In all of 15 childhood patients with TB, the positive rates were 86.7%, 20.0%, 26.7%, 40%, and 66.7% in IGRA, sputum smear, CGA, FQ-PCR, and TST, respectively. In the children vaccinated with BCG, the positive rate of IGRA was significantly lower than that of TST (6.7% vs 76.7%). From high to low, the specificities of the five methods were sputum smear (100%), IGRA (86.7%), FQ-PCR (86.7%), TST (40%), and CGA (26.7%). Although the specificities of sputum smear and FQ-PCR were more than or equal to that of IGRA, the relative sensitivities limited their applications in populations of children. IGRA is a sensitive and specific method, and could be taken as a first choice for detecting MTB infection in populations of children.
authors.affiliation: Clinical Laboratory (JZ, ZZ); Department of Cardiology (CK), Affiliated Hospital of Jining Medical College; Tuberculosis Prevention and Control Institute (YS), Infectious Disease Hospital of Jining City; and Department of Pediatrics (ZY), Affiliated Hospital of Jining Medical College, Jining, Shandong, China.
authors.forename: Jianwei
authors.initials: J
authors.lastname: Zhou
authors.forename: Cui
authors.initials: C
authors.lastname: Kong
authors.forename: Yanxi
authors.initials: Y
authors.lastname: Shi
authors.forename: Zhaocai
authors.initials: Z
authors.lastname: Zhang
authors.forename: Zhaohong
authors.initials: Z
authors.lastname: Yuan
mesh_terms: D000293:Adolescent; D001500:BCG Vaccine; D002648:Child; D002675:Child, Preschool; D005260:Female; D017979:Gold Colloid; D006801:Humans; D059425:Interferon-gamma Release Tests; D008297:Male; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D014374:Tuberculin Test; D014376:Tuberculosis / Q000175:diagnosis*; D014611:Vaccination
chemical_list: D001500:BCG Vaccine; D017979:Gold Colloid; C058457:Aurodye