title: [MALDI-TOF mass-spectrometric analysis of cell proteins during identification of leptospira genus members].
abstract: OBJECTIVE
  Development of methodological approaches for identification of leptospira by using MALDI-TOF direct protein profiling technology.
  
  
  METHODS
  Analysis of cell proteins of 34 leptospira strains was carried out in Microflex LT by using "MALDI Biotyper 3.0 for identification and classification of microorganisms" program.
  
  
  RESULTS
  19 reference spectra of reference leptospira strains from 7 species were generated and imported into MALDI Biotyper 3.0 database. Identification of 6 strains with undetermined taxonomic position was carried out.
  
  
  CONCLUSIONS
  The approved method allows determination of leptospira species with accuracy that depends on their adaptation to nutrient media, preparation approach and sample storage conditions for mass-spectrometry.
authors.forename: N V
authors.initials: NV
authors.lastname: Breneva
authors.forename: M V
authors.initials: MV
authors.lastname: Afanas'ev
authors.forename: M B
authors.initials: MB
authors.lastname: Sharakshanov
authors.forename: A S
authors.initials: AS
authors.lastname: Ostiak
authors.forename: E Iu
authors.initials: EIu
authors.lastname: Kiseleva
authors.forename: A P
authors.initials: AP
authors.lastname: Samsonova
authors.forename: E M
authors.initials: EM
authors.lastname: Petrov
authors.forename: E V
authors.initials: EV
authors.lastname: Viktorova
authors.forename: S V
authors.initials: SV
authors.lastname: Balakhonov
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D007919:Leptospira / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity; D007922:Leptospirosis / Q000235:genetics* / Q000382:microbiology; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
chemical_list: D001426:Bacterial Proteins