title: In Vivo Antiprotozoal Activity of the Chloroform Extract from Carica papaya Seeds against Amastigote Stage of Trypanosoma cruzi during Indeterminate and Chronic Phase of Infection.
abstract: In order to evaluate the antiprotozoal activity of the chloroform extract of Carica papaya seeds during the subacute and chronic phase of infection of Trypanosoma cruzi, doses of 50 and 75 mg/kg were evaluated during the subacute phase, including a mixture of their main components (oleic, palmitic, and stearic acids). Subsequently, doses of 50 and 75 mg/kg in mice during the chronic phase of infection (100 dpi) were also evaluated. It was found that chloroform extract was able to reduce the amastigote nests numbers during the subacute phase in 55.5 and 69.7% (P > 0.05) as well as in 56.45% in animals treated with the mixture of fatty acids. Moreover, the experimental groups treated with 50 and 75 mg/kg during the chronic phase of the infection showed a significant reduction of 46.8 and 53.13% respectively (P < 0.05). It is recommended to carry out more studies to determine if higher doses of chloroformic extract or its administration in combination with other antichagasic drugs allows a better response over the intracellular stage of T. cruzi in infected animal models and determine if the chloroform extract of C. papaya could be considered as an alternative for treatment during the indeterminate and chronic phase of the infection.
authors.affiliation: Laboratorio de Biología Celular, CIR "Dr. Hideyo Noguchi", CA Biomedicina de Enfermedades Infecciosas y Parasitarias, Universidad Autonoma de Yucatán, Avenida Itzaes No. 490 x 59, Centro, 97000 Mérida, YUC, Mexico.
authors.forename: Matilde
authors.identifier: 0000-0002-8802-2049
authors.initials: M
authors.lastname: Jimenez-Coello
authors.affiliation: Laboratorio de Biología Celular, CIR "Dr. Hideyo Noguchi", CA Biomedicina de Enfermedades Infecciosas y Parasitarias, Universidad Autonoma de Yucatán, Avenida Itzaes No. 490 x 59, Centro, 97000 Mérida, YUC, Mexico.
authors.forename: Karla Y
authors.identifier: 0000-0001-7311-0858
authors.initials: KY
authors.lastname: Acosta-Viana
authors.affiliation: Departamento de Salud Animal y Medicina Preventiva, CA Salud Animal, Facultad de Medicina Veterinaria y Zootecnia, Universidad Autónoma de Yucatán, Carretera Mérida-Xmatkuil, Km 15.5 Carr. Merida-Xmatkuil, A.P. 4-116, Mérida, YUC, Mexico.
authors.forename: Antonio
authors.initials: A
authors.lastname: Ortega-Pacheco
authors.affiliation: Universidad Autonoma Metropolitana-Xochimilco, Calzada del Hueso No. 1100, A.P. 23-181, 04960 México, DF, Mexico.
authors.forename: Salud
authors.initials: S
authors.lastname: Perez-Gutierrez
authors.affiliation: Laboratorio de Biología Celular, CIR "Dr. Hideyo Noguchi", CA Biomedicina de Enfermedades Infecciosas y Parasitarias, Universidad Autonoma de Yucatán, Avenida Itzaes No. 490 x 59, Centro, 97000 Mérida, YUC, Mexico.
authors.forename: Eugenia
authors.initials: E
authors.lastname: Guzman-Marin