title: Pepper, sweet (Capsicum annuum).
abstract: Capsicum (pepper) species are economically important crops that are recalcitrant to genetic transformation by Agrobacterium (Agrobacterium tumefaciens). A number of protocols for pepper transformation have been described but are not routinely applicable. The main bottleneck in pepper transformation is the low frequency of cells that are both susceptible for Agrobacterium infection and have the ability to regenerate. Here, we describe a protocol for the efficient regeneration of transgenic sweet pepper (C. annuum) through inducible activation of the BABY BOOM (BBM) AP2/ERF transcription factor. Using this approach, we can routinely achieve a transformation efficiency of at least 0.6 %. The main improvements in this protocol are the reproducibility in transforming different genotypes and the ability to produce fertile shoots. An added advantage of this protocol is that BBM activity can be induced subsequently in stable transgenic lines, providing a novel regeneration system for clonal propagation through somatic embryogenesis.
authors.affiliation: Enza Zaden Research and Development B.V., P.O. Box 7, 1600 AA, Enkhuizen, The Netherlands.
authors.forename: Iris
authors.initials: I
authors.lastname: Heidmann
authors.forename: Kim
authors.initials: K
authors.lastname: Boutilier
mesh_terms: D016960:Agrobacterium tumefaciens / Q000235:genetics; D002212:Capsicum / Q000235:genetics* / Q000254:growth & development*; D005821:Genetic Techniques; D008967:Molecular Biology / Q000379:methods*; D030821:Plants, Genetically Modified; D012639:Seeds / Q000235:genetics / Q000254:growth & development; D013242:Sterilization; D014157:Transcription Factors / Q000235:genetics; D014169:Transformation, Bacterial
chemical_list: D014157:Transcription Factors