title: Electro-acupuncture at Neiguan pretreatment alters genome-wide gene expressions and protects rat myocardium against ischemia-reperfusion.
abstract: This study investigated genome-wide gene expressions and the cardioprotective effects of electro-acupuncture pretreatment at the PC6 Neiguan acupoint on myocardial ischemia reperfusion (I/R) injury. Male SD rats were randomly divided into four groups: sham operation (SO), I/R, electro-acupuncture at the PC6 Neiguan acupoint pretreatment (EA) and electro-acupuncture at non-acupoint pretreatment (NA). Compared with the I/R group, the survival rate of the EA group was significantly increased, the arrhythmia score, infarction area, serum concentrations of CK, LDH and CK-Mb and plasma level of cTnT were significantly decreased. RNA-seq results showed that 725 genes were up-regulated and 861 genes were down-regulated under I/R conditions compared to the SO group; both EA and NA reversed some of these gene expression levels (592 in EA and 238 in NA group). KEGG pathway analysis indicated that these genes were involved in multiple pathways, including ECM, MAPK signaling, apoptosis, cytokine and leukocyte pathways. In addition, some pathways were uniquely regulated by EA, but not NA pretreatment, such as oxidative stress, cardiac muscle contraction, gap junction, vascular smooth muscle contraction, hypertrophic, NOD-like receptor, and P53 and B-cell receptor pathways. This study was first to reveal the gene expression signatures of acute myocardial I/R injury and electro-acupuncture pretreatment in rats.
authors.affiliation: Key Laboratory of Acupuncture and Medicine Research of Ministry of Education, Nanjing University of Chinese Medicine, No. 138, Xianlin Rd, Nanjing 210023, Jiangsu, China. hyan0915@sina.com.
authors.forename: Yan
authors.initials: Y
authors.lastname: Huang
authors.affiliation: Key Laboratory of Acupuncture and Medicine Research of Ministry of Education, Nanjing University of Chinese Medicine, No. 138, Xianlin Rd, Nanjing 210023, Jiangsu, China. lushengfeng2013@gmail.com.
authors.forename: Sheng-Feng
authors.initials: SF
authors.lastname: Lu
authors.affiliation: School of Information Technology, Nanjing University of Chinese Medicine, No. 138, Xianlin Rd, Nanjing 210023, Jiangsu, China. hucjhyl@126.com.
authors.forename: Chen-Jun
authors.initials: CJ
authors.lastname: Hu
authors.affiliation: Key Laboratory of Acupuncture and Medicine Research of Ministry of Education, Nanjing University of Chinese Medicine, No. 138, Xianlin Rd, Nanjing 210023, Jiangsu, China. shuping.fu@gmail.com.
authors.forename: Shu-Ping
authors.initials: SP
authors.lastname: Fu
authors.affiliation: Key Laboratory of Acupuncture and Medicine Research of Ministry of Education, Nanjing University of Chinese Medicine, No. 138, Xianlin Rd, Nanjing 210023, Jiangsu, China. weixingshen@sina.cn.
authors.forename: Wei-Xing
authors.initials: WX
authors.lastname: Shen
authors.affiliation: Key Laboratory of Acupuncture and Medicine Research of Ministry of Education, Nanjing University of Chinese Medicine, No. 138, Xianlin Rd, Nanjing 210023, Jiangsu, China. wliu@vet.upenn.edu.
authors.forename: Wan-Xin
authors.initials: WX
authors.lastname: Liu
authors.affiliation: Key Laboratory of Acupuncture and Medicine Research of Ministry of Education, Nanjing University of Chinese Medicine, No. 138, Xianlin Rd, Nanjing 210023, Jiangsu, China. liqian20140603@163.com.
authors.forename: Qian
authors.initials: Q
authors.lastname: Li
authors.affiliation: Key Laboratory of Acupuncture and Medicine Research of Ministry of Education, Nanjing University of Chinese Medicine, No. 138, Xianlin Rd, Nanjing 210023, Jiangsu, China. hebei@263.net.
authors.forename: Ning
authors.initials: N
authors.lastname: Wang
authors.affiliation: Key Laboratory of Acupuncture and Medicine Research of Ministry of Education, Nanjing University of Chinese Medicine, No. 138, Xianlin Rd, Nanjing 210023, Jiangsu, China. weixiaosoon@163.com.
authors.forename: Su-Yun
authors.initials: SY
authors.lastname: He
authors.affiliation: School of Acupuncture and Tuina, Chengdu University of Traditional Chinese Medicine, No.1166, Liutai Avenue, Wenjiang District, Chengdu 611137, Sichuan, China. acu973@126.com.
authors.forename: Fan-Rong
authors.initials: FR
authors.lastname: Liang
authors.affiliation: Key Laboratory of Acupuncture and Medicine Research of Ministry of Education, Nanjing University of Chinese Medicine, No. 138, Xianlin Rd, Nanjing 210023, Jiangsu, China. zhubm64@hotmail.com.
authors.forename: Bing-Mei
authors.initials: BM
authors.lastname: Zhu
mesh_terms: D015670:Acupuncture Therapy / Q000379:methods; D000818:Animals; D016000:Cluster Analysis; D004195:Disease Models, Animal; D004562:Electrocardiography; D015870:Gene Expression; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D055106:Genome-Wide Association Study; D008297:Male; D015428:Myocardial Reperfusion Injury / Q000235:genetics* / Q000378:metabolism / Q000401:mortality / Q000517:prevention & control / Q000628:therapy*; D051381:Rats; D015203:Reproducibility of Results; D015398:Signal Transduction