title: Transcriptomic profiling of human choriodecidua during term labor: inflammation as a key driver of labor.
abstract: OBJECTIVE
  Inflammation is a driver of labor in myometrium and cervix; however, the involvement of decidua is poorly defined. We have reported decidual leukocyte infiltration prior to and during labor; the regulators of these inflammatory processes are unknown.
  
  
  METHODS
  Choriodecidua RNA obtained after term labor or elective cesarean delivery was applied to Affymetrix GeneChips. Pathway analysis and gene validation were performed.
  
  
  RESULTS
  Extensive inflammatory activation was identified in choriodecidua following labor, predominantly upregulation of genes regulating leukocyte trafficking and cytokine signalling. Genes governing cell fate, tissue remodelling, and translation were also altered. Upregulation of candidate genes (ICAM1, CXCR4, CD44, TLR4, SOCS3, BCL2A, and IDO) was confirmed. NFκB, STAT1&3, HMGB1, and miRNA-21, miRNA-46, miRNA-141, and miRNA-200 were predicted upstream regulators.
  
  
  CONCLUSIONS
  This study confirms inflammatory processes are major players in labor events in choriodecidua, as in other gestational tissues. Suppressing uterine inflammation is likely to be critical for arresting premature labor.
authors.affiliation: Maternal and Fetal Health Research Centre, University of Manchester, Manchester, UK; St. Mary's Hospital, Central Manchester University Hospitals NHS Foundation Trust, Manchester Academic Health Science Centre, Manchester, UK.
authors.forename: Gillian L
authors.initials: GL
authors.lastname: Stephen
authors.forename: Sylvia
authors.initials: S
authors.lastname: Lui
authors.forename: Sarah A
authors.initials: SA
authors.lastname: Hamilton
authors.forename: Clare L
authors.initials: CL
authors.lastname: Tower
authors.forename: Lynda K
authors.initials: LK
authors.lastname: Harris
authors.forename: Adam
authors.initials: A
authors.lastname: Stevens
authors.forename: Rebecca L
authors.initials: RL
authors.lastname: Jones
mesh_terms: D002465:Cell Movement / Q000235:genetics; D002823:Chorion / Q000502:physiology*; D016207:Cytokines / Q000378:metabolism; D003656:Decidua / Q000502:physiology*; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D007249:Inflammation / Q000235:genetics / Q000276:immunology*; D018836:Inflammation Mediators / Q000378:metabolism; D007745:Labor Onset / Q000276:immunology; D007743:Labor, Obstetric / Q000235:genetics / Q000276:immunology*; D007962:Leukocytes / Q000276:immunology*; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism; D046228:Microarray Analysis; D011247:Pregnancy; D015398:Signal Transduction / Q000235:genetics / Q000276:immunology; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D015854:Up-Regulation
chemical_list: D016207:Cytokines; D018836:Inflammation Mediators; D035683:MicroRNAs; D014157:Transcription Factors
keywords: Chemokines; choriodecidua; miRNAs; pathway analysis; transcription factors