title: Electrochemical aptasensor for mucin 1 based on dual signal amplification of poly(o-phenylenediamine) carrier and functionalized carbon nanotubes tracing tag.
abstract: Mucin 1 (MUC 1), as a most studied mucin, has become a useful marker for identifying breast cancer in the early stages. In this work, a novel method for the determination of MUC 1 in serum was developed based on a sandwich-type electrochemical aptasensor, which combined a dual signal amplification strategy of poly(o-phenylenediamine)-Au nanoparticles (PoPD-AuNPs) hybrid film as carrier and AuNPs functionalized silica/multiwalled carbon nanotubes core-shell nanocomposites (AuNPs/SiO2@MWCNTs) as tracing tag. The PoPD-AuNPs film provides a suitable microenvironment for stabilizing the primary aptamer (Apt) assembly, and the AuNPs/SiO2@MWCNTs enhances the surface area for immobilizing abundant secondary Apts as well as load large amounts of electrochemical probe thionine (Thi). In the presence of MUC 1, the sandwich-type recognition reacted on the aptasensor surface, and the Thi-AuNPs/SiO2@MWCNTs nanoprobes were captured onto the electrode surface to form biocomplex. AuNPs and MWCNTs could facilitate the electron transfer from Thi to the electrode, thus amplifying the detection response. Under the optimized experimental conditions, the proposed sensing strategy provided a wider linear dynamic range over three orders of magnitude with the detection limit down to 1 pM. Moreover, the aptasensor demonstrated good precision, acceptable stability and reproducibility.
authors.affiliation: College of Sciences, Nanjing Tech University, Nanjing 211816, PR China. Electronic address: chenxj_njut@126.com.
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Chen
authors.affiliation: College of Sciences, Nanjing Tech University, Nanjing 211816, PR China; Geological Survey of Jiangsu Province, Nanjing 210018, PR China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: College of Sciences, Nanjing Tech University, Nanjing 211816, PR China.
authors.forename: Chunhua
authors.initials: C
authors.lastname: Qian
authors.affiliation: Biotechnology and Pharmaceutical Engineering,Nanjing Tech University, Nanjing 211816, PR China.
authors.forename: Ning
authors.initials: N
authors.lastname: Hao
authors.affiliation: Biotechnology and Pharmaceutical Engineering,Nanjing Tech University, Nanjing 211816, PR China.
authors.forename: Lin
authors.initials: L
authors.lastname: Xu
authors.affiliation: College of Sciences, Nanjing Tech University, Nanjing 211816, PR China. Electronic address: yaocheng@njtech.edu.cn.
authors.forename: Cheng
authors.initials: C
authors.lastname: Yao
mesh_terms: D052157:Aptamers, Nucleotide / Q000032:analysis / Q000737:chemistry* / Q000235:genetics; D015374:Biosensing Techniques / Q000295:instrumentation*; D003217:Conductometry / Q000295:instrumentation*; D004595:Electroplating; D004867:Equipment Design; D019544:Equipment Failure Analysis; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D018396:Mucin-1 / Q000032:analysis* / Q000737:chemistry / Q000235:genetics; D037742:Nanotubes, Carbon / Q000737:chemistry* / Q000648:ultrastructure; D010655:Phenylenediamines / Q000737:chemistry*
chemical_list: D052157:Aptamers, Nucleotide; D018396:Mucin-1; D037742:Nanotubes, Carbon; D010655:Phenylenediamines; C530487:poly(o-phenylenediamine)
keywords: Electrochemical aptasensor; Functionalized carbon nanotubes; Mucin 1; Poly(o-phenylenediamine); Sandwich-type