title: Mammalian cell display technology coupling with AID induced SHM in vitro: an ideal approach to the production of therapeutic antibodies.
abstract: Traditional antibody production technology within non-mammalian cell expression systems has shown many unsatisfactory properties for the development of therapeutic antibodies. Nevertheless, mammalian cell display technology reaps the benefits of producing full-length all human antibodies. Together with the developed cytidine deaminase induced in vitro somatic hypermutation technology, mammalian cell display technology provides the opportunity to produce high affinity antibodies that might be ideal for therapeutic application. This review was concentrated on the development of the mammalian cell display technology as well as the activation-induced cytidine deaminase induced in vitro somatic hypermutation technology and their applications for the production of therapeutic antibodies.
authors.affiliation: Tumor Immunobiology Laboratory of Cancer Research Institute, Cancer Research Institution, Central South University, 410078, Xiangya Street, Changsha, Hunan, China.
authors.forename: Chang-Fei
authors.initials: CF
authors.lastname: Qin
authors.affiliation: Tumor Immunobiology Laboratory of Cancer Research Institute, Cancer Research Institution, Central South University, 410078, Xiangya Street, Changsha, Hunan, China. Electronic address: libsun@163.com.
authors.forename: Guan-Cheng
authors.initials: GC
authors.lastname: Li
mesh_terms: D000818:Animals; D000906:Antibodies / Q000378:metabolism* / Q000627:therapeutic use; D002460:Cell Line; D061505:Cell Surface Display Techniques / Q000379:methods*; D003564:Cytidine Deaminase / Q000235:genetics / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000502:physiology*; D008322:Mammals; D027041:Somatic Hypermutation, Immunoglobulin / Q000502:physiology*; D000098830:AICDA (Activation-Induced Cytidine Deaminase)
chemical_list: D000906:Antibodies; D000098830:AICDA (Activation-Induced Cytidine Deaminase); D003564:Cytidine Deaminase
keywords: Activation-induced cytidine deaminase; Mammalian cell display; Somatic hypermutation; Therapeutic antibody