title: Endogenous hormones response to cytokinins with regard to organogenesis in explants of peach (Prunus persica L. Batsch) cultivars and rootstocks (P. persica × Prunus dulcis).
abstract: Organogenesis in peach (Prunus persica L. Batsch) and peach rootstocks (P. persica × Prunus dulcis) has been achieved and the action of the regeneration medium on 7 phytohormones, zeatin (Z), zeatin riboside (ZR), indole-3-acetic acid (IAA), abscisic acid (ABA), ethylene precursor 1-aminocyclopropane-1-carboxylic acid (ACC), salicylic acid (SA), and jasmonic acid (JA), has been studied using High performance liquid chromatography - mass spectrometry (HPLC-MS/MS). Three scion peach cultivars, 'UFO-3', 'Flariba' and 'Alice Bigi', and the peach × almond rootstocks 'Garnem' and 'GF677' were cultured in two different media, Murashige and Skoog supplemented with plant growth regulators (PGRs) (regeneration medium) and without PGRs (control medium), in order to study the effects of the media and/or genotypes in the endogenous hormones content and their role in organogenesis. The highest regeneration rate was obtained with the peach × almond rootstocks and showed a lower content of Z, IAA, ABA, ACC and JA. Only Z, ZR and IAA were affected by the action of the culture media. This study shows which hormones are external PGRs-dependent and what is the weight of the genotype and hormones in peach organogenesis that provide an avenue to manipulate in vitro organogenesis in peach.
authors.affiliation: Departamento de Hortofruticultura, Instituto Murciano de Investigación y Desarrollo Agrario y Alimentario (IMIDA), C/ Mayor s/n, 30150 La Alberca, Murcia, Spain. Electronic address: margarita.perez3@carm.es.
authors.forename: Margarita
authors.initials: M
authors.lastname: Pérez-Jiménez
authors.affiliation: Departamento de Nutrición Vegetal, Centro de Edafología y Biología Aplicada del Segura (CEBAS), Consejo Superior de Investigaciones Científicas (CSIC), Campus Universitario de Espinardo, 30100 Murcia, Spain.
authors.forename: Elena
authors.initials: E
authors.lastname: Cantero-Navarro
authors.affiliation: Departamento de Nutrición Vegetal, Centro de Edafología y Biología Aplicada del Segura (CEBAS), Consejo Superior de Investigaciones Científicas (CSIC), Campus Universitario de Espinardo, 30100 Murcia, Spain.
authors.forename: Francisco
authors.initials: F
authors.lastname: Pérez-Alfocea
authors.affiliation: Departamento de Hortofruticultura, Instituto Murciano de Investigación y Desarrollo Agrario y Alimentario (IMIDA), C/ Mayor s/n, 30150 La Alberca, Murcia, Spain.
authors.forename: José
authors.initials: J
authors.lastname: Cos-Terrer
mesh_terms: D000040:Abscisic Acid / Q000378:metabolism; D000598:Amino Acids, Cyclic / Q000378:metabolism; D003517:Cyclopentanes / Q000378:metabolism; D003583:Cytokinins / Q000494:pharmacology*; D007210:Indoleacetic Acids / Q000378:metabolism; D007541:Isopentenyladenosine / Q000031:analogs & derivatives / Q000378:metabolism; D038081:Organogenesis / Q000187:drug effects; D054883:Oxylipins / Q000378:metabolism; D010937:Plant Growth Regulators / Q000378:metabolism*; D027861:Prunus / Q000187:drug effects / Q000378:metabolism*; D020156:Salicylic Acid / Q000378:metabolism; D015026:Zeatin / Q000378:metabolism
chemical_list: D000598:Amino Acids, Cyclic; D003517:Cyclopentanes; D003583:Cytokinins; D007210:Indoleacetic Acids; D054883:Oxylipins; D010937:Plant Growth Regulators; C023863:1-aminocyclopropane-1-carboxylic acid; C011006:jasmonic acid; C030737:indoleacetic acid; D000040:Abscisic Acid; D007541:Isopentenyladenosine; D015026:Zeatin; C009699:zeatin riboside; D020156:Salicylic Acid
keywords: 1-Aminocyclopropane-1-carboxylic acid; Abscisic acid; Indole-3-acetic acid; Morphogenesis; Zeatin; Zeatin riboside