title: Biocompatibility of quantum dots (CdSe/ZnS ) in human amniotic membrane-derived mesenchymal stem cells in vitro.
abstract: OBJECTIVE
  Amniotic membrane-derived mesenchymal stem cells (hAM-dMSCs) are a potential source of mesenchymal stem cells which could be used to repair skin damage. The use of mesenchymal stem cells to repair skin damage requires safe, effective and biocompatible agents to evaluate the effectiveness of the result. Quantum dots (QDs) composed of CdSe/ZnS are semiconductor nanocrystals with broad excitation and narrow emission spectra, which have been considered as a new chemical and fluorescent substance for non-invasively labeling different cells in vitro and in vivo. This study investigated the cytotoxic effects of QDs on hAM-dMSCs at different times following labeling.
  
  
  METHODS
  Using 0.75, 1.5 and 3.0 μL between quantum dots, labeled human amniotic mesenchymal stem cells were collected on days 1, 2 and 4 and observed morphological changes, performed an MTT cell growth assay and flow cytometry for mesenchymal stem cells molecular markers.
  
  
  RESULTS
  Quantum dot concentration 0.75 μg/mL labeled under a fluorescence microscope, cell morphology was observed, The MTT assay showed cells in the proliferative phase. Flow cytometry expression CD29, CD31, CD34, CD44, CD90, CD105 and CD106.
  
  
  CONCLUSIONS
  Within a certain range of concentrations between quantum dots labeled human amniotic mesenchymal stem cells has good biocompatibility.
authors.forename: Gongping
authors.initials: G
authors.lastname: Wang
authors.forename: Guangwei
authors.initials: G
authors.lastname: Zeng
authors.affiliation: Department of Pharmacy, the First Affiliated Hospital of Henan University of Science and Technology, LuoYang 471003 , Henan Province, PR China.
authors.forename: Caie
authors.initials: C
authors.lastname: Wang
authors.affiliation: Department of Colorectal Surgery, People Hospital of Zhengzhou, Zhengzhou 450000 , Henan Province, PR China.
authors.forename: Huasheng
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of Neurosurgery, the First Affiliated Hospital of ZhengZhou University, ZhengZho 450001, Henan Province, PR China.
authors.forename: Bo
authors.initials: B
authors.lastname: Yang
authors.affiliation: Henan Academy of Medical Sciences, ZhengZhou 450001, Henan Province, PR China.
authors.forename: Fangxia
authors.initials: F
authors.lastname: Guan
authors.affiliation: Department of Emergency, the First Affiliated Hospital of Henan University of Science and Technology, LuoYang 471003 , Henan Province, PR China.
authors.forename: Dongpeng
authors.initials: D
authors.lastname: Li
authors.affiliation: Department of Oncological Surgery, the First Affiliated Hospital of Henan University of Science and Technology, LuoYang 471003 , Henan Province, PR China.
authors.forename: Xiaoshan
authors.initials: X
authors.lastname: Feng
mesh_terms: D000650:Amnion / Q000166:cytology; D001672:Biocompatible Materials / Q000378:metabolism*; D001681:Biological Assay / Q000379:methods; D006801:Humans; D066298:In Vitro Techniques; D008422:Materials Testing; D059630:Mesenchymal Stem Cells / Q000378:metabolism*; D008856:Microscopy, Fluorescence / Q000379:methods; D045663:Quantum Dots / Q000009:adverse effects* / Q000378:metabolism; D013194:Staining and Labeling / Q000379:methods
chemical_list: D001672:Biocompatible Materials
keywords: biocompatibility; human amniotic membrane; mesenchymal stem cells; quantum dots