title: Inhibition of Adipogenesis by Oligonol through Akt-mTOR Inhibition in 3T3-L1 Adipocytes.
abstract: Polyphenols have recently become an important focus of study in obesity research. Oligonol is an oligomerized polyphenol, typically comprised of catechin-type polyphenols from a variety of fruits, which has been found to exhibit better bioavailability and bioreactivity than natural polyphenol compounds. Here, we demonstrated that Oligonol inhibits 3T3-L1 adipocyte differentiation by reducing adipogenic gene expression. During adipogenesis, Oligonol downregulated the mRNA levels of peroxisome proliferator-activated receptor γ (PPARγ), CCAAT/enhancer binding proteins α (C/EBPα), and δ (C/EBPδ) in a dose-dependent manner and the expression of genes involved in lipid biosynthesis. The antiadipogenic effect of Oligonol appears to originate from its ability to inhibit the Akt and mammalian target of rapamycin (mTOR) signaling pathway by diminishing the phosphorylation of ribosomal protein S6 kinase (p70S6K), a downstream target of mTOR and forkhead box protein O1 (Foxo1). These results suggest that Oligonol may be a potent regulator of obesity by repressing major adipogenic genes through inhibition of the Akt signaling pathway, which induces the inhibition of lipid accumulation, ultimately inhibiting adipogenesis.
authors.affiliation: Department of Clinical Nursing Science, Yonsei University College of Nursing, Seoul 120-752, Republic of Korea ; Nursing Policy and Research Institute, Biobehavioral Research Center, Yonsei University, Seoul 120-752, Republic of Korea ; Division of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul 136-701, Republic of Korea.
authors.forename: Jae-Yeo
authors.initials: JY
authors.lastname: Park
authors.affiliation: Department of Emergency Medical Technology, Kyungil Univeristy, Gyeongsan, Kyungbook 712-701, Republic of Korea.
authors.forename: Younghwa
authors.initials: Y
authors.lastname: Kim
authors.affiliation: Sport and Medicine Research Center, INTOTO Inc., 401 Dawoo BD, 90-6 Daeshin-Dong, Seodaemun-Gu, Seoul 120-160, Republic of Korea.
authors.forename: Jee Ae
authors.initials: JA
authors.lastname: Im
authors.affiliation: Division of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul 136-701, Republic of Korea.
authors.forename: Seungkwon
authors.initials: S
authors.lastname: You
authors.affiliation: Department of Clinical Nursing Science, Yonsei University College of Nursing, Seoul 120-752, Republic of Korea ; Nursing Policy and Research Institute, Biobehavioral Research Center, Yonsei University, Seoul 120-752, Republic of Korea.
authors.forename: Hyangkyu
authors.identifier: 0000-0002-0821-6020
authors.initials: H
authors.lastname: Lee