title: CHD1 and CHD2 are positive regulators of HIV-1 gene expression.
abstract: BACKGROUND
  Retroviruses encode a very limited number of proteins and therefore must exploit a wide variety of host proteins for completion of their lifecycle.
  
  
  METHODS
  We performed an insertional mutagenesis screen to identify novel cellular regulators of retroviral replication.
  
  
  RESULTS
  This approach identified the ATP-dependent chromatin remodeler, chromodomain helicase DNA-binding protein 2 (CHD2), as well as the highly related CHD1 protein, as positive regulators of both MLV and HIV-1 replication in rodent and human cells. RNAi knockdown of either CHD2 or the related CHD1 protein, in human cells resulted in a block to infection by HIV-1, specifically at the level of transcription.
  
  
  CONCLUSIONS
  These results demonstrate that CHD1 and CHD2 can act as positive regulators of HIV-1 gene expression.
authors.forename: Melissa J
authors.initials: MJ
authors.lastname: Rodgers
authors.forename: David J
authors.initials: DJ
authors.lastname: Banks
authors.forename: Kenneth A
authors.initials: KA
authors.lastname: Bradley
authors.affiliation: The Nomis Center for Immunobiology and Microbial Pathogenesis, The Salk Institute for Biological Studies, 10010 North Torrey Pines Road, La Jolla, CA 92037, USA. jyoung@salk.edu.
authors.forename: John At
authors.initials: JA
authors.lastname: Young
mesh_terms: D004265:DNA Helicases / Q000235:genetics / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D015967:Gene Expression Regulation, Viral; D015658:HIV Infections / Q000201:enzymology* / Q000235:genetics / Q000821:virology; D015497:HIV-1 / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: C575189:CHD2 protein, human; D004268:DNA-Binding Proteins; D014764:Viral Proteins; D004265:DNA Helicases; C080359:CHD1 protein, human