title: HILIC-MS for metabolomics: An attractive and complementary approach to RPLC-MS.
abstract: Hydrophilic interaction chromatography (HILIC) is an emerging separation mode of liquid chromatography (LC). Using highly hydrophilic stationary phases capable of retaining polar/ionic metabolites, and accompany with high organic content mobile phase that offer readily compatibility with mass spectrometry (MS) has made HILIC an attractive complementary tool to the widely used reverse-phase (RP) chromatographic separations in metabolomic studies. The combination of HILIC and RPLC coupled with an MS detector expands the number of detected analytes and provides more comprehensive metabolite coverage than use of only RP chromatography. This review describes the recent applications of HILIC-MS/MS in metabolomic studies, ranging from amino acids, lipids, nucleotides, organic acids, pharmaceuticals, and metabolites of specific nature. The biological systems investigated include microbials, cultured cell line, plants, herbal medicine, urine, and serum as well as tissues from animals and humans. Owing to its unique capability to measure more-polar biomolecules, the HILIC separation technique would no doubt enhance the comprehensiveness of metabolite detection, and add significant value for metabolomic investigations. © 2014 Wiley Periodicals, Inc. Mass Spec Rev 35:574-600, 2016.
authors.affiliation: Department of Pharmaceutical Analysis, Xuzhou Medical College, Xuzhou, 221044, China.|Jiangsu Key Lab for the study of New Drug and Clinical Pharmacy, Xuzhou Medical College, Yunlong, China.|NUS Environmental Research Inst., National University of Singapore, 5 A Engineering Srive 1, Singapore, 117411, Singapore.
authors.forename: Dao-Quan
authors.initials: DQ
authors.lastname: Tang
authors.affiliation: Saw Swee Hock School of Public Health, National University of Singapore, 16 Medical Drive, Singapore, 117597, Singapore.
authors.forename: Ll
authors.initials: L
authors.lastname: Zou
authors.affiliation: Jiangsu Key Lab for the study of New Drug and Clinical Pharmacy, Xuzhou Medical College, Yunlong, China.
authors.forename: Xiao-Xing
authors.initials: XX
authors.lastname: Yin
authors.affiliation: NUS Environmental Research Inst., National University of Singapore, 5 A Engineering Srive 1, Singapore, 117411, Singapore.|Saw Swee Hock School of Public Health, National University of Singapore, 16 Medical Drive, Singapore, 117597, Singapore.
authors.forename: Choon Nam
authors.initials: CN
authors.lastname: Ong
mesh_terms: D000818:Animals; D002853:Chromatography, Liquid; D056148:Chromatography, Reverse-Phase; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D055432:Metabolomics; D053719:Tandem Mass Spectrometry
keywords: hydrophilic interaction chromatography; mass spectrometry; metabolic-profiling; polar compounds; reverse-phase chromatography