title: Templated DNA ligation with thiol chemistry.
abstract: We describe two DNA-templated ligation strategies: native chemical ligation (NCL), and thiol-disulfide exchange. Both systems result in successful ligation in the presence of a DNA template. The stability of the product from the NCL reaction relies on exogenous thiol, while the thiol-disulfide reaction proceeds in a catalyst-free manner.
authors.affiliation: Department of Chemistry, New York University, 100 Washington Sq. East, New York, NY 10003, USA. james.canary@nyu.edu ned.seeman@nyu.edu.
authors.forename: Dadong
authors.initials: D
authors.lastname: Li
authors.forename: Xiaojian
authors.initials: X
authors.lastname: Wang
authors.forename: Fubo
authors.initials: F
authors.lastname: Shi
authors.forename: Ruojie
authors.initials: R
authors.lastname: Sha
authors.forename: Nadrian C
authors.initials: NC
authors.lastname: Seeman
authors.forename: James W
authors.initials: JW
authors.lastname: Canary
mesh_terms: D004247:DNA / Q000737:chemistry*; D004220:Disulfides / Q000737:chemistry; D015394:Molecular Structure; D013438:Sulfhydryl Compounds / Q000737:chemistry*
chemical_list: D004220:Disulfides; D013438:Sulfhydryl Compounds; D004247:DNA