title: Creatine metabolism differs between mammals and rainbow trout (Oncorhynchus mykiss).
abstract: Creatine plays an important role in the cell as an energy buffer. As the energy system is a basic element of the organism it may possibly contribute to differences between rainbow trout strains selected for the traits growth and robustness, respectively. The cDNA sequences of creatine-related genes encoding glycine amidinotransferase (GATM), guanidinoacetate N-methyltransferase (GAMT), creatine kinase muscle-type (CKM) and creatine transporter 1 (CT1, encoded by gene solute carrier family 6, member 8 (SLC6A8)) were characterized in rainbow trout. Transcripts of the respective genes were quantified in kidney, liver, brain and skeletal muscle in both trout strains that had been acclimated to different temperatures. Several differences between the compared trout strains were found as well as between temperatures indicating that the energy system may contribute to differences between both strains. In addition to that, the expression data showed clear differences between the creatine system in rainbow trout and mammals, as the spatial distribution of the enzyme-encoding gene expression was clearly different from the patterns described for mammals. In rainbow trout, creatine synthesis seems to take place to a big extent in the skeletal muscle.
authors.affiliation: Leibniz-Institut für Nutztierbiologie (FBN), Institut für Genombiologie, Wilhelm-Stahl-Allee 2, Dummerstorf, 18196 Germany.
authors.forename: Andreas
authors.initials: A
authors.lastname: Borchel
authors.affiliation: Leibniz-Institut für Nutztierbiologie (FBN), Institut für Genombiologie, Wilhelm-Stahl-Allee 2, Dummerstorf, 18196 Germany.
authors.forename: Marieke
authors.initials: M
authors.lastname: Verleih
authors.affiliation: Leibniz-Institut für Nutztierbiologie (FBN), Institut für Genombiologie, Wilhelm-Stahl-Allee 2, Dummerstorf, 18196 Germany.
authors.forename: Alexander
authors.initials: A
authors.lastname: Rebl
authors.affiliation: Landesforschungsanstalt für Landwirtschaft und Fischerei Mecklenburg-Vorpommern (LFA M-V), Institut für Fischerei, Born, Germany.
authors.forename: Carsten
authors.initials: C
authors.lastname: Kühn
authors.affiliation: Leibniz-Institut für Nutztierbiologie (FBN), Institut für Genombiologie, Wilhelm-Stahl-Allee 2, Dummerstorf, 18196 Germany.
authors.forename: Tom
authors.initials: T
authors.lastname: Goldammer
keywords: Creatine kinase brain-type (CKB); Creatine kinase muscle-type (CKM); Energy metabolism; L-arginine:glycine amidinotransferase (GATM); Rainbow trout; S-adenosylmethionine: guanidinoacetate N-methyltransferase (GAMT); Teleost