title: The fertility of the hybrid lineage derived from female Megalobrama amblycephala × male Culter alburnus.
abstract: Distant hybridization can combine together the genomes of different species, which leads to changes of the offspring in phenotypes and genotypes. In this study, we successfully establish a fertile hybrid lineage by intergeneric hybridization of female blunt snout bream (BSB, Megalobrama amblycephala) × male topmouth culter (TC, Culter alburnus) and investigate some important biological traits of this lineage including the morphological traits, chromosomal number, karyotype, DNA content, gonadal development, egg and milt yield, sperm shape and density, fertilization rate and early survival rate. The results show that: (1) the diploid and triploid hybrids coexist in F1 and only diploid hybrids are found in F2, in which the diploid hybrids of F1 and F2 possess 48 chromosomes with one chromosome set of BSB and one chromosome set of TC, and the triploid hybrids of F1 possess 72 chromosomes with two chromosome sets of BSB and one chromosome set of TC. (2) All the tested males and females of the diploid F1 and F2 hybrids have the normal gonadal development and produce mature sperm and egg, respectively, which are fertilized with each other to form F2 and F3 hybrids, respectively, and finally form a diploid hybrid lineage (F1-F3). (3) The good fertility of the F1 and F2 hybrids of female BSB × male TC potentially provides reproductive base to make the hybrid lineage propagate from one generation to another. The formation of the hybrid lineage (F1-F3) also provides an ideal model to research the reproductive rules of distant hybrid progeny.
authors.affiliation: Key Laboratory of Protein Chemistry and Fish Developmental Biology of the Education Ministry of China, College of Life Sciences, Hunan Normal University, 36 Lu Shan Road, Yue Lu District, Changsha 410081, Hunan, People's Republic of China.
authors.forename: Jun
authors.initials: J
authors.lastname: Xiao
authors.affiliation: Key Laboratory of Protein Chemistry and Fish Developmental Biology of the Education Ministry of China, College of Life Sciences, Hunan Normal University, 36 Lu Shan Road, Yue Lu District, Changsha 410081, Hunan, People's Republic of China.
authors.forename: Xuewei
authors.initials: X
authors.lastname: Kang
authors.affiliation: Key Laboratory of Protein Chemistry and Fish Developmental Biology of the Education Ministry of China, College of Life Sciences, Hunan Normal University, 36 Lu Shan Road, Yue Lu District, Changsha 410081, Hunan, People's Republic of China.
authors.forename: Lihua
authors.initials: L
authors.lastname: Xie
authors.affiliation: Key Laboratory of Protein Chemistry and Fish Developmental Biology of the Education Ministry of China, College of Life Sciences, Hunan Normal University, 36 Lu Shan Road, Yue Lu District, Changsha 410081, Hunan, People's Republic of China.
authors.forename: Qinbo
authors.initials: Q
authors.lastname: Qin
authors.affiliation: Key Laboratory of Protein Chemistry and Fish Developmental Biology of the Education Ministry of China, College of Life Sciences, Hunan Normal University, 36 Lu Shan Road, Yue Lu District, Changsha 410081, Hunan, People's Republic of China.
authors.forename: Zhoulin
authors.initials: Z
authors.lastname: He
authors.affiliation: Key Laboratory of Protein Chemistry and Fish Developmental Biology of the Education Ministry of China, College of Life Sciences, Hunan Normal University, 36 Lu Shan Road, Yue Lu District, Changsha 410081, Hunan, People's Republic of China.
authors.forename: Fangzhou
authors.initials: F
authors.lastname: Hu
authors.affiliation: Key Laboratory of Protein Chemistry and Fish Developmental Biology of the Education Ministry of China, College of Life Sciences, Hunan Normal University, 36 Lu Shan Road, Yue Lu District, Changsha 410081, Hunan, People's Republic of China.
authors.forename: Chun
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Protein Chemistry and Fish Developmental Biology of the Education Ministry of China, College of Life Sciences, Hunan Normal University, 36 Lu Shan Road, Yue Lu District, Changsha 410081, Hunan, People's Republic of China.
authors.forename: Rurong
authors.initials: R
authors.lastname: Zhao
authors.affiliation: Key Laboratory of Protein Chemistry and Fish Developmental Biology of the Education Ministry of China, College of Life Sciences, Hunan Normal University, 36 Lu Shan Road, Yue Lu District, Changsha 410081, Hunan, People's Republic of China.
authors.forename: Jun
authors.initials: J
authors.lastname: Wang
authors.affiliation: Key Laboratory of Protein Chemistry and Fish Developmental Biology of the Education Ministry of China, College of Life Sciences, Hunan Normal University, 36 Lu Shan Road, Yue Lu District, Changsha 410081, Hunan, People's Republic of China.
authors.forename: Kaikun
authors.initials: K
authors.lastname: Luo
authors.affiliation: Key Laboratory of Protein Chemistry and Fish Developmental Biology of the Education Ministry of China, College of Life Sciences, Hunan Normal University, 36 Lu Shan Road, Yue Lu District, Changsha 410081, Hunan, People's Republic of China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Key Laboratory of Protein Chemistry and Fish Developmental Biology of the Education Ministry of China, College of Life Sciences, Hunan Normal University, 36 Lu Shan Road, Yue Lu District, Changsha 410081, Hunan, People's Republic of China. Electronic address: lsj@hunnu.edu.cn.
authors.forename: Shaojun
authors.initials: S
authors.lastname: Liu
mesh_terms: D000818:Animals; D005260:Female; D005298:Fertility / Q000235:genetics*; D006824:Hybridization, Genetic; D059785:Karyotype; D008297:Male; D010473:Perciformes / Q000235:genetics*; D011003:Ploidies
keywords: Diploid and triploid hybrid; Distant hybridization; Fertility; Hybrid lineage