title: Expression levels of TWIST1 are associated with the clinicopathological stage of B-cell non-Hodgkin lymphoma.
abstract: The aim of the present study was to investigate the expression level of TWIST1 in B-cell non-Hodgkin lymphoma (BNHL) and its association with the clinicopathological characteristics of BNHL. Expression levels of TWIST1 were analyzed in patients with BNHL (n=45) and lymphadenosis (n=21) using immunohistochemical staining and western blot analysis. In addition, the mRNA expression levels of TWIST1 in the peripheral blood were detected by fluorescent quantitative polymerase chain reaction. The positive rate of TWIST1 expression in the BNHL tissue was 82.2%, which was significantly higher compared with the lymphadenosis tissue (5%; P<0.05). In addition, the protein expression level of TWIST1 in the BNHL tissue was higher compared with the lymphadenosis tissue. TWIST1 expression was also higher in stage III/IV BNHL tissues than in stage I/II tissues (P<0.05). The tissues were staged following the Ann Arbor system. Furthermore, the mRNA expression level of TWIST1 in the peripheral blood of the BNHL tissue (3.03±0.03) was higher compared with the lymphadenosis tissue, and the mRNA expression level of TWIST1 was higher in stage III/IV (4.41±0.12) tissues than in stage I/II BNHL (2.03±0.08) tissues. In conclusion, TWIST1 expression was higher in the tissue and peripheral blood of patients with BNHL when compared with those with lymphadenosis. Thus, TWIST1 expression was associated with the clinicopathological stage of BNHL.
authors.affiliation: Department of Medical Oncology, The Affiliated Tumor Hospital of Xinjiang Medical University, Ürümqi, Xinjiang 830011, P.R. China.
authors.forename: Cundong
authors.initials: C
authors.lastname: Jia
authors.affiliation: Department of Pathology, The Affiliated Tumor Hospital of Xinjiang Medical University, Ürümqi, Xinjiang 830011, P.R. China.
authors.forename: Liping
authors.initials: L
authors.lastname: Liang
authors.affiliation: Department of Pathology, The Affiliated Tumor Hospital of Xinjiang Medical University, Ürümqi, Xinjiang 830011, P.R. China.
authors.forename: Lili
authors.initials: L
authors.lastname: Yang
authors.affiliation: Department of Pathology, The Affiliated Tumor Hospital of Xinjiang Medical University, Ürümqi, Xinjiang 830011, P.R. China.
authors.forename: Feng
authors.initials: F
authors.lastname: Zhao
authors.affiliation: Department of Bone Oncology, The Affiliated Tumor Hospital of Xinjiang Medical University, Ürümqi, Xinjiang 830011, P.R. China.
authors.forename: Jingping
authors.initials: J
authors.lastname: Bai
keywords: B cell non-Hodgkin; TWIST1; immunohistochemistry staining; lymphoma