title: In vivo monitoring of rat macrophages labeled with poly(l-lysine)-iron oxide nanoparticles.
abstract: Coprecipitation of FeCl2 and FeCl3 with aqueous ammonia was used to prepare iron oxide nanoparticles dispersible in aqueous medium. Oxidation of the particles with sodium hypochlorite then yielded maghemite (γ-Fe2 O3 ) nanoparticles which were coated with two types of coating -d-mannose or poly(l-lysine) (PLL) as confirmed by FTIR analysis. The particles were <10 nm according to transmission electron microscopy. Their hydrodynamic particle size was ∼180 nm (by dynamic light scattering). The d-mannose-, PLL-coated, and neat γ-Fe2 O3 particles as well as commercial Resovist® were used to label rat macrophages. The viability and contrast properties of labeled macrophages were compared. PLL-coated γ-Fe2 O3 nanoparticles were found optimal. The labeled macrophages were injected to rats monitored in vivo by magnetic resonance imaging up to 48 h. Transport of macrophages labeled with PLL-γ-Fe2 O3 nanoparticles in rats was confirmed. Tracking of macrophages using the developed particles can be used for monitoring of inflammations and cell migration in cell therapy.
authors.affiliation: Institute of Macromolecular Chemistry, Department of Polymer Particles, Academy of Sciences of the Czech Republic, 162 06, Prague 6, Czech Republic.
authors.forename: Michal
authors.initials: M
authors.lastname: Babič
authors.affiliation: Institute for Clinical and Experimental Medicine, Laboratory for Atherosclerosis Research, 140 21, Prague 4, Czech Republic.
authors.forename: Martina
authors.initials: M
authors.lastname: Schmiedtová
authors.affiliation: Institute for Clinical and Experimental Medicine, Laboratory for Atherosclerosis Research, 140 21, Prague 4, Czech Republic.
authors.forename: Rudolf
authors.initials: R
authors.lastname: Poledne
authors.affiliation: Institute for Clinical and Experimental Medicine, Laboratory for Atherosclerosis Research, 140 21, Prague 4, Czech Republic.|Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Vídeňská, 1083, Czech Republic.
authors.forename: Vít
authors.initials: V
authors.lastname: Herynek
authors.affiliation: Institute of Macromolecular Chemistry, Department of Polymer Particles, Academy of Sciences of the Czech Republic, 162 06, Prague 6, Czech Republic.
authors.forename: Daniel
authors.initials: D
authors.lastname: Horák
mesh_terms: D000818:Animals; D058948:Cell Tracking / Q000379:methods*; D003287:Contrast Media / Q000737:chemistry / Q000494:pharmacology; D005290:Ferric Compounds / Q000737:chemistry / Q000494:pharmacology; D008264:Macrophages / Q000000981:diagnostic imaging*; D008279:Magnetic Resonance Imaging / Q000379:methods*; D008297:Male; D053758:Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D011107:Polylysine / Q000737:chemistry / Q000494:pharmacology; D011859:Radiography; D051381:Rats
chemical_list: D003287:Contrast Media; D005290:Ferric Compounds; C000499:ferric oxide; D011107:Polylysine
keywords: MRI; iron oxide; labeling; macrophages; nanoparaticles