title: Protective effect of colchicine on ovarian ischemia-reperfusion injury: an experimental study.
abstract: OBJECTIVE
  The aim of the present study is to investigate the efficiency of colchicine in the experimental rat ovarian torsion model in the light of histological and biochemical data.
  
  
  METHODS
  A total of 35 Wistar albino female rats were randomly divided into 5 groups, group 1: (control-sham operated, n = 7); group 2: (torsion/detorsion, n = 7) 2 hours of ischemia and 2 hours of reperfusion; group 3: (torsion/detorsion, n = 7), 2 hours of ischemia and 5 days of reperfusion; group 4: (torsion/detorsion, n = 7) 2 hours of ischemia and 2 hours of reperfusion and a signal dose of oral 1 mL/kg colchicine; and group 5: (torsion/detorsion, n = 7), 2 hours of ischemia and 5 days of reperfusion and 5 days of oral 1 mg/kg colchicine. Histopathologic evaluation was performed by a scoring that assesses congestion, bleeding, edema, and cellular degeneration in the ovarian tissue. Catalase, tissue malondialdehyde (MDA), and protein carbonyl levels were calculated.
  
  
  RESULTS
  The histopathologic scores, MDA, and protein carbonyl levels in the control and colchicine groups were significantly lower than groups 2 and 3 (P < .001). Catalase activities were significantly higher in the control and colchicine groups than in groups 2 and 3 (P < .001). The results of the histopathologic parameters and biochemical markers showed that protective effects of colchicine treatment persisted up to 5 days.
  
  
  CONCLUSIONS
  Our study results revealed that colchicine reduced ovarian ischemia-reperfusion injury in experimental rat ovarian torsion model. As the ovarian detorsion is the first choice of the treatment modality in the early phase, antioxidant and anti-inflammatory treatment modalities like colchicine might be used to reduce ovarian ischemia-reperfusion injury.
authors.affiliation: Mustafa Kemal University Medical School, Department of Obstetrics and Gynecology, Hatay, Turkey drraziyekeskinkurt@yahoo.com.
authors.forename: Raziye Keskin
authors.initials: RK
authors.lastname: Kurt
authors.affiliation: Zekai Tahir Burak Women's Health Training and Research Hospital, Ankara, Turkey.
authors.forename: Ayse Citil
authors.initials: AC
authors.lastname: Dogan
authors.affiliation: Hacettepe University Medical School, Department of Physiology, Ankara, Turkey.
authors.forename: Murat
authors.initials: M
authors.lastname: Dogan
authors.affiliation: Ankara Diskapi YB Training and Research Hospital, Department of Pathology, Ankara, Turkey.
authors.forename: Aynur
authors.initials: A
authors.lastname: Albayrak
authors.affiliation: Hacettepe University Medical School, Department of Physiology, Ankara, Turkey.
authors.forename: Sefika Nur
authors.initials: SN
authors.lastname: Kurt
authors.affiliation: Hacettepe University Medical School, Department of Physiology, Ankara, Turkey.
authors.forename: Furkan
authors.initials: F
authors.lastname: Eren
authors.affiliation: Mustafa Kemal University Medical School, Department of Obstetrics and Gynecology, Hatay, Turkey.
authors.forename: Ayse Guler
authors.initials: AG
authors.lastname: Okyay
authors.affiliation: Mustafa Kemal University Medical School, Department of Obstetrics and Gynecology, Hatay, Turkey.
authors.forename: Atilla
authors.initials: A
authors.lastname: Karateke
authors.affiliation: Mustafa Kemal University Medical School, Department of Emergency Medicine, Hatay, Turkey.
authors.forename: Mehmet
authors.initials: M
authors.lastname: Duru
authors.affiliation: Hacettepe University Medical School, Department of Physiology, Ankara, Turkey.
authors.forename: Ersin
authors.initials: E
authors.lastname: Fadillioglu
authors.affiliation: Ankara Diskapi YB Training and Research Hospital, Department of Endocrinology and Metabolism, Ankara, Turkey Ankara Diskapi YB Training and Research Hospital, Pancreas Islet Research Center, Ankara, Turkey Hacettepe University Kastamonu Medical School, Department of Endocrinology and Metabolism, Ankara, Turkey.
authors.forename: Tuncay
authors.initials: T
authors.lastname: Delibasi
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology*; D002374:Catalase / Q000378:metabolism; D003078:Colchicine / Q000494:pharmacology*; D019610:Cytoprotection; D004195:Disease Models, Animal; D005260:Female; D008315:Malondialdehyde / Q000378:metabolism; D010049:Ovarian Diseases / Q000150:complications / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D010053:Ovary / Q000098:blood supply / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D050050:Protein Carbonylation; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000209:etiology / Q000378:metabolism / Q000473:pathology / Q000628:therapy*; D013997:Time Factors; D014102:Torsion Abnormality / Q000150:complications / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology
chemical_list: D000893:Anti-Inflammatory Agents; D000975:Antioxidants; D008315:Malondialdehyde; D002374:Catalase; D003078:Colchicine
keywords: colchicine; ischemia–reperfusion injury; ovarian torsion