title: Structure of a short-chain dehydrogenase/reductase (SDR) within a genomic island from a clinical strain of Acinetobacter baumannii.
abstract: Over 15% of the genome of an Australian clinical isolate of Acinetobacter baumannii occurs within genomic islands. An uncharacterized protein encoded within one island feature common to this and other International Clone II strains has been studied by X-ray crystallography. The 2.4 Å resolution structure of SDR-WM99c reveals it to be a new member of the classical short-chain dehydrogenase/reductase (SDR) superfamily. The enzyme contains a nucleotide-binding domain and, like many other SDRs, is tetrameric in form. The active site contains a catalytic tetrad (Asn117, Ser146, Tyr159 and Lys163) and water molecules occupying the presumed NADP cofactor-binding pocket. An adjacent cleft is capped by a relatively mobile helical subdomain, which is well positioned to control substrate access.
authors.affiliation: Department of Chemistry and Biomolecular Sciences, Macquarie University, Research Park Drive, Sydney, NSW 2109, Australia.
authors.forename: Bhumika S
authors.initials: BS
authors.lastname: Shah
authors.affiliation: Department of Chemistry and Biomolecular Sciences, Macquarie University, Research Park Drive, Sydney, NSW 2109, Australia.
authors.forename: Sasha G
authors.initials: SG
authors.lastname: Tetu
authors.affiliation: School of Physics, University of New South Wales, Sydney, NSW 2052, Australia.
authors.forename: Stephen J
authors.initials: SJ
authors.lastname: Harrop
authors.affiliation: Department of Chemistry and Biomolecular Sciences, Macquarie University, Research Park Drive, Sydney, NSW 2109, Australia.
authors.forename: Ian T
authors.initials: IT
authors.lastname: Paulsen
authors.affiliation: Department of Chemistry and Biomolecular Sciences, Macquarie University, Research Park Drive, Sydney, NSW 2109, Australia.
authors.forename: Bridget C
authors.initials: BC
authors.lastname: Mabbutt
mesh_terms: D040981:Acinetobacter baumannii / Q000201:enzymology* / Q000235:genetics; D000595:Amino Acid Sequence; D001051:Apoenzymes / Q000737:chemistry; D001426:Bacterial Proteins / Q000737:chemistry*; D020134:Catalytic Domain; D018360:Crystallography, X-Ray; D064429:Fatty Acid Synthases / Q000737:chemistry*; D016680:Genome, Bacterial; D044404:Genomic Islands; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009247:NADH, NADPH Oxidoreductases / Q000737:chemistry*
chemical_list: D001051:Apoenzymes; D001426:Bacterial Proteins; C036543:short chain trans-2-enoyl-CoA reductase; D009247:NADH, NADPH Oxidoreductases; D064429:Fatty Acid Synthases
keywords: Acinetobacter baumannii WM99c; Rossmann fold; multidrug resistance; nosocomial strain; opportunistic pathogen