title: Selective Interaction Between Chloroplast β-ATPase and TGB1L88 Retards Severe Symptoms Caused by Alternanthera mosaic virus Infection.
abstract: The multifunctional triple gene block protein 1 (TGB1) of the Potexvirus Alternanthera mosaic virus (AltMV) has been reported to have silencing suppressor, cell-to-cell movement, and helicase functions. Yeast two hybrid screening using an Arabidopsis thaliana cDNA library with TGB1 as bait, and co-purification with TGB1 inclusion bodies identified several host proteins which interact with AltMV TGB1. Host protein interactions with TGB1 were confirmed by biomolecular fluorescence complementation, which showed positive TGB1 interaction with mitochondrial ATP synthase delta' chain subunit (ATP synthase delta'), light harvesting chlorophyll-protein complex I subunit A4 (LHCA4), chlorophyll a/b binding protein 1 (LHB1B2), chloroplast-localized IscA-like protein (ATCPISCA), and chloroplast β-ATPase. However, chloroplast β-ATPase interacts only with TGB1L88, and not with weak silencing suppressor TGB1P88. This selective interaction indicates that chloroplast β-ATPase is not required for AltMV movement and replication; however, TRV silencing of chloroplast β-ATPase in Nicotiana benthamiana induced severe tissue necrosis when plants were infected by AltMV TGB1L88 but not AltMV TGB1P88, suggesting that β-ATPase selectively responded to TGB1L88 to induce defense responses.
authors.affiliation: Department of Applied Biology, Chungnam National University, Daejeon 305-764, Korea.
authors.forename: Eun-Young
authors.initials: EY
authors.lastname: Seo
authors.affiliation: Department of Applied Biology, Chungnam National University, Daejeon 305-764, Korea ; Department of Bioscience II, Bio-Medical Science, Daejeon 305-301, Korea.
authors.forename: Jiryun
authors.initials: J
authors.lastname: Nam
authors.affiliation: Department of Applied Biology, Chungnam National University, Daejeon 305-764, Korea.
authors.forename: Hyun-Seung
authors.initials: HS
authors.lastname: Kim
authors.affiliation: School of Biotechnology, Yeungnam University, Gyeongsan 712-749, Korea.
authors.forename: Young-Hwan
authors.initials: YH
authors.lastname: Park
authors.affiliation: Department of Medicine, Graduate School, Chung-Ang University, Seoul 156-756, Korea.
authors.forename: Seok Myeong
authors.initials: SM
authors.lastname: Hong
authors.affiliation: USDA-ARS, US National Arboretum, Floral and Nursery Plants Research Unit, Beltsville, MD 20705, USA.
authors.forename: Dilip
authors.initials: D
authors.lastname: Lakshman
authors.affiliation: School of Biotechnology, Yeungnam University, Gyeongsan 712-749, Korea.
authors.forename: Hanhong
authors.initials: H
authors.lastname: Bae
authors.affiliation: USDA-ARS, US National Arboretum, Floral and Nursery Plants Research Unit, Beltsville, MD 20705, USA.
authors.forename: John
authors.initials: J
authors.lastname: Hammond
authors.affiliation: Department of Applied Biology, Chungnam National University, Daejeon 305-764, Korea.
authors.forename: Hyoun-Sub
authors.initials: HS
authors.lastname: Lim
keywords: AltMV; Chloroplast β-ATPase; TGB1