title: A new ochratoxin A biodegradation strategy using Cupriavidus basilensis Őr16 strain.
abstract: Ochratoxin-A (OTA) is a mycotoxin with possibly carcinogenic and nephrotoxic effects in humans and animals. OTA is often found as a contaminant in agricultural commodities. The aim of the present work was to evaluate OTA-degrading and detoxifying potential of Cupriavidus basilensis ŐR16 strain. In vivo administration of OTA in CD1 male mice (1 or 10 mg/kg body weight for 72 hours or 0.5 mg/kg body weight for 21 days) resulted in significant elevation of OTA levels in the blood, histopathological alterations- and transcriptional changes in OTA-dependent genes (annexinA2, clusterin, sulphotransferase and gadd45 and gadd153) in the renal cortex. These OTA-induced changes were not seen in animals that have been treated with culture supernatants in which OTA was incubated with Cupriavidus basilensis ŐR16 strain for 5 days. HPLC and ELISA methods identified ochratoxin α as the major metabolite of OTA in Cupriavidus basilensis ŐR16 cultures, which is not toxic in vivo. This study has demonstrated that Cupriavidus basilensis ŐR16 efficiently degrade OTA without producing toxic adventitious metabolites.
authors.affiliation: Institute of Experimental Medicine, Laboratory of Molecular Neuroendocrinology, Budapest, Hungary.
authors.forename: Szilamér
authors.initials: S
authors.lastname: Ferenczi
authors.affiliation: Szent István University, Department of Environmental Protection and Safety, Gödöllő, Hungary.
authors.forename: Mátyás
authors.initials: M
authors.lastname: Cserháti
authors.affiliation: Szent István University, Department of Environmental Protection and Safety, Gödöllő, Hungary.
authors.forename: Csilla
authors.initials: C
authors.lastname: Krifaton
authors.affiliation: Szent István University, Department of Environmental Protection and Safety, Gödöllő, Hungary.
authors.forename: Sándor
authors.initials: S
authors.lastname: Szoboszlay
authors.affiliation: Central Environmental and Food Science Research Institute, Department of Microbiology, Budapest, Hungary.
authors.forename: József
authors.initials: J
authors.lastname: Kukolya
authors.affiliation: Soft Flow Hungary R&D Ltd., Pécs, Hungary.
authors.forename: Zsuzsanna
authors.initials: Z
authors.lastname: Szőke
authors.affiliation: Soft Flow Hungary R&D Ltd., Pécs, Hungary.
authors.forename: Balázs
authors.initials: B
authors.lastname: Kőszegi
authors.affiliation: CEVA Phylaxia Ltd, Budapest, Hungary.
authors.forename: Mihály
authors.initials: M
authors.lastname: Albert
authors.affiliation: University of Debrecen, Department of Genetics and Applied Microbiology, Debrecen, Hungary.
authors.forename: Teréz
authors.initials: T
authors.lastname: Barna
authors.affiliation: Szent István University, Department of Nutrition, Gödöllő, Hungary.
authors.forename: Miklós
authors.initials: M
authors.lastname: Mézes
authors.affiliation: Institute of Experimental Medicine, Laboratory of Molecular Neuroendocrinology, Budapest, Hungary.
authors.forename: Krisztina J
authors.initials: KJ
authors.lastname: Kovács
authors.affiliation: Szent István University, Department of Environmental Protection and Safety, Gödöllő, Hungary.
authors.forename: Balázs
authors.initials: B
authors.lastname: Kriszt
mesh_terms: D000818:Animals; D001673:Biodegradation, Environmental; D002273:Carcinogens / Q000633:toxicity*; D049921:Cupriavidus / Q000378:metabolism*; D007668:Kidney / Q000187:drug effects; D008297:Male; D051379:Mice; D009183:Mycotoxins / Q000378:metabolism / Q000633:toxicity*; D009793:Ochratoxins / Q000378:metabolism / Q000633:toxicity*
chemical_list: D002273:Carcinogens; D009183:Mycotoxins; D009793:Ochratoxins; C025589:ochratoxin A