title: Multiplex PCR for detection of seven virulence factors and K1/K2 capsular serotypes of Klebsiella pneumoniae.
abstract: A single multiplex PCR assay targeting seven virulence factors and the wzi gene specific for the K1 and K2 capsular serotypes of Klebsiella pneumoniae was developed and tested on 65 clinical isolates, which included 45 isolates responsible for community-acquired severe human infections. The assay is useful for the surveillance of emerging highly virulent strains.
authors.affiliation: Sorbonne Universités, UPMC Univ Paris 06 CR7, Centre d'Immunologie et des Maladies Infectieuses, CIMI, Team E13 (Bacteriology), Paris, France INSERM U1135, Centre d'Immunologie et des Maladies Infectieuses, CIMI, Team E13 (Bacteriology), Paris, France compain.fabrice@wanadoo.fr.
authors.forename: Fabrice
authors.initials: F
authors.lastname: Compain
authors.affiliation: Sorbonne Universités, UPMC Univ Paris 06 CR7, Centre d'Immunologie et des Maladies Infectieuses, CIMI, Team E13 (Bacteriology), Paris, France INSERM U1135, Centre d'Immunologie et des Maladies Infectieuses, CIMI, Team E13 (Bacteriology), Paris, France.
authors.forename: Ana
authors.initials: A
authors.lastname: Babosan
authors.affiliation: Institut Pasteur, Microbial Evolutionary Genomics, Paris, France CNRS, UMR3525, Paris, France.
authors.forename: Sylvain
authors.initials: S
authors.lastname: Brisse
authors.affiliation: Sorbonne Universités, UPMC Univ Paris 06 CR7, Centre d'Immunologie et des Maladies Infectieuses, CIMI, Team E13 (Bacteriology), Paris, France INSERM U1135, Centre d'Immunologie et des Maladies Infectieuses, CIMI, Team E13 (Bacteriology), Paris, France.
authors.forename: Nathalie
authors.initials: N
authors.lastname: Genel
authors.affiliation: Sorbonne Universités, UPMC Univ Paris 06 CR7, Centre d'Immunologie et des Maladies Infectieuses, CIMI, Team E13 (Bacteriology), Paris, France INSERM U1135, Centre d'Immunologie et des Maladies Infectieuses, CIMI, Team E13 (Bacteriology), Paris, France.
authors.forename: Jennifer
authors.initials: J
authors.lastname: Audo
authors.affiliation: Institut Pasteur, Microbial Evolutionary Genomics, Paris, France CNRS, UMR3525, Paris, France.
authors.forename: Florent
authors.initials: F
authors.lastname: Ailloud
authors.affiliation: Sorbonne Universités, UPMC Univ Paris 06 CR7, Centre d'Immunologie et des Maladies Infectieuses, CIMI, Team E13 (Bacteriology), Paris, France INSERM U1135, Centre d'Immunologie et des Maladies Infectieuses, CIMI, Team E13 (Bacteriology), Paris, France.
authors.forename: Najiby
authors.initials: N
authors.lastname: Kassis-Chikhani
authors.affiliation: Sorbonne Universités, UPMC Univ Paris 06 CR7, Centre d'Immunologie et des Maladies Infectieuses, CIMI, Team E13 (Bacteriology), Paris, France INSERM U1135, Centre d'Immunologie et des Maladies Infectieuses, CIMI, Team E13 (Bacteriology), Paris, France AP-HP Département de Bactériologie, Hôpitaux de l'Est Parisien, APHP, Paris, France.
authors.forename: Guillaume
authors.initials: G
authors.lastname: Arlet
authors.affiliation: Sorbonne Universités, UPMC Univ Paris 06 CR7, Centre d'Immunologie et des Maladies Infectieuses, CIMI, Team E13 (Bacteriology), Paris, France INSERM U1135, Centre d'Immunologie et des Maladies Infectieuses, CIMI, Team E13 (Bacteriology), Paris, France AP-HP Département de Bactériologie, Hôpitaux de l'Est Parisien, APHP, Paris, France.
authors.forename: Dominique
authors.initials: D
authors.lastname: Decré
mesh_terms: D000942:Antigens, Bacterial / Q000235:genetics*; D000954:Antigens, Surface / Q000235:genetics*; D001431:Bacteriological Techniques / Q000379:methods*; D006801:Humans; D007710:Klebsiella Infections / Q000382:microbiology; D007711:Klebsiella pneumoniae / Q000235:genetics* / Q000302:isolation & purification; D017720:Molecular Epidemiology / Q000379:methods; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods*; D037521:Virulence Factors / Q000235:genetics*
chemical_list: D000942:Antigens, Bacterial; D000954:Antigens, Surface; C030659:K antigens; D037521:Virulence Factors