title: Expression of RINT1 predicts seizure occurrence and outcomes in patients with low-grade gliomas.
abstract: OBJECTIVE
  Most patients with low-grade gliomas (LGGs) experience epileptic seizures as an initial symptom. However, the mechanism of LGG-related epilepsy is poorly understood. Genetic changes in brain tumors influence epileptic seizures, but few biomarkers have been associated with LGG-related seizures. We investigated the association between LGG-related epilepsy and tumor-specific molecular changes.
  
  
  METHODS
  Clinical characteristics, RNA sequence data, and case follow-up data were reviewed for 76 patients with histologically confirmed LGG. Gene expression (n = 21,469) was compared between patients with preoperative epileptic seizures and those without preoperative epileptic seizures. The Engel classification was used at 6 months after surgery to evaluate the prognostic role of genes that passed the screen.
  
  
  RESULTS
  Expression of RAD50 interactor 1 (RINT1) significantly differed between LGG patients with and without preoperative epileptic seizures (p = 0.003). This result was validated by applying the same analysis to RNA sequence data from The Cancer Genome Atlas (p = 0.048). Patients with high RINT1 expression were at increased risk of LGG-related seizures compared to those with low expression (p = 0.044). RINT1 was also identified as a predictor of seizure outcomes in patients with LGG at 6 months after tumor resection (p = 0.022).
  
  
  CONCLUSIONS
  Our results suggest that high RINT1 expression may represent a risk factor for LGG-related seizures and may be associated with seizure outcomes.
authors.affiliation: Beijing Neurosurgical Institute, Capital Medical University, No. 6 Tiantan Xili, Dongcheng District, Beijing, 100050, China.
authors.forename: Xing
authors.initials: X
authors.lastname: Fan
authors.forename: Yin-yan
authors.initials: YY
authors.lastname: Wang
authors.forename: Chuan-bao
authors.initials: CB
authors.lastname: Zhang
authors.forename: Gan
authors.initials: G
authors.lastname: You
authors.forename: Ming-yang
authors.initials: MY
authors.lastname: Li
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.forename: Tao
authors.initials: T
authors.lastname: Jiang
mesh_terms: D000328:Adult; D018797:Cell Cycle Proteins / Q000235:genetics*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D020022:Genetic Predisposition to Disease / Q000235:genetics*; D005910:Glioma / Q000150:complications / Q000235:genetics* / Q000601:surgery; D006801:Humans; D016015:Logistic Models; D008297:Male; D008875:Middle Aged; D015999:Multivariate Analysis; D060787:Neoplasm Grading; D011237:Predictive Value of Tests; D057234:Preoperative Period; D011379:Prognosis; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012307:Risk Factors; D012640:Seizures / Q000150:complications / Q000235:genetics*; D016896:Treatment Outcome; D055815:Young Adult
chemical_list: D018797:Cell Cycle Proteins; C422909:RINT1 protein, human; D012333:RNA, Messenger