title: Backbone and side chain NMR assignment, along with the secondary structure prediction of RRM2 domain of La protein from a lower eukaryote exhibiting identical structural organization with its human homolog.
abstract: The La protein (Lupus antigen), a key mediator during biogenesis of RNA polymerase III transcripts, contains a characteristic La motif and one or two RNA recognition motif (RRM) domains, depending on the organism of origin. The RRM1 domain is conserved in higher eukaryotes and located in the N-terminal region, whereas the C-terminal RRM2 domain is absent in most lower eukaryotes and its specific role remains, so far, uncharacterized. Here, we present the backbone and side-chain assignment of the (1)H, (13)C and (15)N resonances of RRM2 of La protein from Dictyostelium discoideum. Interestingly, the La protein in this lower eukaryote, exhibits high homology to its human counterpart. Moreover, it contains two RRM domains, instead of one, raising questions on its evolutionary origin and the putative role of RRM2 in vivo. We also provide its secondary structure as predicted by the TALOS+ online tool.
authors.affiliation: Department of Pharmacy, University of Patras, 26504, Patras, Greece.
authors.forename: Aikaterini I
authors.initials: AI
authors.lastname: Argyriou
authors.forename: Christos T
authors.initials: CT
authors.lastname: Chasapis
authors.forename: Maria
authors.initials: M
authors.lastname: Apostolidi
authors.forename: Parthena
authors.initials: P
authors.lastname: Konstantinidou
authors.forename: Constantinos
authors.initials: C
authors.lastname: Stathopoulos
authors.forename: Detlef
authors.initials: D
authors.lastname: Bentrop
authors.forename: Georgios A
authors.initials: GA
authors.lastname: Spyroulias
mesh_terms: D004023:Dictyostelium; D006801:Humans; D019906:Nuclear Magnetic Resonance, Biomolecular; D010750:Phosphoproteins / Q000737:chemistry*; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D015800:Protozoan Proteins / Q000737:chemistry*; D017386:Sequence Homology, Amino Acid
chemical_list: C574674:La protein, human; D010750:Phosphoproteins; D015800:Protozoan Proteins