title: Wheat (Triticum aestivum L.) transformation using mature embryos.
abstract: In most protocols for the Agrobacterium-mediated transformation of wheat, the preferred target tissues are immature embryos. However, transformation methods relying on immature embryos require the growth of plants under controlled conditions to provide a continuous supply of good-quality target tissue. The use of mature embryos as a target tissue has the advantage of only requiring good-quality seed as the starting material. Here we describe a transformation method based on the Agrobacterium-mediated transformation of callus cultures derived from mature wheat embryos of the genotype Bobwhite S56.
authors.affiliation: Department of Crop Genetics, John Innes Centre, Norwich Research Park, Norwich, NR4 7UH, UK, eva.medvecka@ist.ac.at.
authors.forename: Eva
authors.initials: E
authors.lastname: Medvecká
authors.forename: Wendy A
authors.initials: WA
authors.lastname: Harwood
mesh_terms: D016960:Agrobacterium tumefaciens / Q000235:genetics; D018920:Coculture Techniques; D005821:Genetic Techniques; D018525:Germination; D030821:Plants, Genetically Modified; D012639:Seeds / Q000235:genetics*; D014169:Transformation, Bacterial; D014908:Triticum / Q000235:genetics* / Q000254:growth & development