title: Cationic micellar nanoparticles for DNA and doxorubicin co-delivery.
abstract: Cationic micellar nanoparticles for chemotherapeutic drugs and therapeutic gene co-delivery were prepared based on a poly-(N-ε-carbobenzyloxy-l-lysine) (PZLL) and dendritic polyamidoamine (PAMAM) block copolymer (PZLL-D3). PZLL-D3 was synthesized by a copper-catalyzed azide alkyne cyclization (click) reaction between α-alkyne-PZLL and azide focal point PAMAM dendrons. Its structure was characterized by (1)H NMR and FTIR, and its buffering capability was determined by acid-base titration. MTT, agarose gel electrophoresis and flow cytometry studies showed that PZLL-D3 revealed low in vitro cytotoxicity, strong pDNA condensation ability, protection of pDNA against deoxyribonuclease I degradation and high gene transfection efficiency in 293T and HeLa cells. In addition, the micellar nanoparticles delivered pDNA and anticancer drug doxorubicin (DOX) simultaneously and efficiently to tumor cells, and the DOX loaded nanoparticles showed sustained in vitro release at pH=7.4 and 5.8.
authors.affiliation: Traditional Chinese Medicine and New Drug Research Institute, Guangdong Medical College, Dongguan 523808, China. Electronic address: linjt326@163.com.
authors.forename: Jian-Tao
authors.initials: JT
authors.lastname: Lin
authors.affiliation: Department of Traditional Chinese Medicine, The Second Clinical Medical College, Guangdong Medical College, Dongguan 523808, China. Electronic address: cz98@163.com.
authors.forename: Ying
authors.initials: Y
authors.lastname: Zou
authors.affiliation: Department of Chemistry, Virginia Tech, Blacksburg, VA 24061, United States. Electronic address: cwang09@vt.edu.
authors.forename: Chao
authors.initials: C
authors.lastname: Wang
authors.affiliation: Traditional Chinese Medicine and New Drug Research Institute, Guangdong Medical College, Dongguan 523808, China. Electronic address: zhongyuechun0101@163.com.
authors.forename: Yue-Chun
authors.initials: YC
authors.lastname: Zhong
authors.affiliation: Department of Microbiology and Immunology, School of Basic Medicine, Guangdong Medical College, Dongguan 523808, China. Electronic address: zhaoyicomnet@gmail.com.
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Emergency Department, The Third Affiliated Hospital of Guangzhou Medical University, Guangzhou 510150, China. Electronic address: zhuhuier123@163.com.
authors.forename: Hui-Er
authors.initials: HE
authors.lastname: Zhu
authors.affiliation: Traditional Chinese Medicine and New Drug Research Institute, Guangdong Medical College, Dongguan 523808, China. Electronic address: wanggh0101@163.com.
authors.forename: Guan-Hai
authors.initials: GH
authors.lastname: Wang
authors.affiliation: DSAPM Lab, PCFM Lab, Institute of Polymer Science, School of Chemistry and Chemical Engineering, Sun Yat-sen University, Guangzhou 510275, China. Electronic address: ceszhlm@mail.sysu.edu.cn.
authors.forename: Li-Ming
authors.initials: LM
authors.lastname: Zhang
authors.affiliation: Department of Traditional Chinese Medicine, The Second Clinical Medical College, Guangdong Medical College, Dongguan 523808, China. Electronic address: xuebaozheng@sina.com.
authors.forename: Xue-Bao
authors.initials: XB
authors.lastname: Zheng
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology; D001672:Biocompatible Materials / Q000737:chemistry / Q000494:pharmacology; D002412:Cations / Q000737:chemistry; D045744:Cell Line, Tumor; D050091:Dendrimers / Q000737:chemistry / Q000494:pharmacology; D003850:Deoxyribonuclease I / Q000378:metabolism; D004317:Doxorubicin / Q000737:chemistry* / Q000494:pharmacology; D016503:Drug Delivery Systems / Q000379:methods*; D006367:HeLa Cells; D006801:Humans; D006863:Hydrogen-Ion Concentration; D009682:Magnetic Resonance Spectroscopy; D008823:Micelles; D053758:Nanoparticles / Q000737:chemistry*; D011107:Polylysine / Q000737:chemistry / Q000494:pharmacology; D011108:Polymers / Q000737:chemistry / Q000494:pharmacology; D017550:Spectroscopy, Fourier Transform Infrared; D014162:Transfection
chemical_list: D000970:Antineoplastic Agents; D001672:Biocompatible Materials; D002412:Cations; D050091:Dendrimers; D008823:Micelles; C104700:PAMAM Starburst; D011108:Polymers; C507445:oligo(N(6)-carbobenzyloxy-L-lysine); D011107:Polylysine; D004317:Doxorubicin; D003850:Deoxyribonuclease I
keywords: Co-delivery; Drug; Gene; PAMAM