title: A highly sensitive europium nanoparticle-based immunoassay for detection of influenza A/B virus antigen in clinical specimens.
abstract: We report the development of a novel europium nanoparticle-based immunoassay (ENIA) for rapid detection of influenza A and influenza B viruses. The ENIA demonstrated sensitivities of 90.7% (147/162) for influenza A viruses and 81.80% (9/11) for influenza B viruses compared to those for an in-house reverse transcription (RT)-PCR assay in testing of influenza-positive clinical samples.
authors.affiliation: Laboratory of Molecular Virology, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, Maryland, USA.
authors.forename: Panhe
authors.initials: P
authors.lastname: Zhang
authors.affiliation: Laboratory of Molecular Virology, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, Maryland, USA saivikram.vemula@fda.hhs.gov indira.hewlett@fda.hhs.gov.
authors.forename: Sai Vikram
authors.initials: SV
authors.lastname: Vemula
authors.affiliation: Laboratory of Molecular Virology, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, Maryland, USA.
authors.forename: Jiangqin
authors.initials: J
authors.lastname: Zhao
authors.affiliation: Laboratory of Molecular Virology, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, Maryland, USA.
authors.forename: Bingchen
authors.initials: B
authors.lastname: Du
authors.affiliation: Laboratory of Molecular Virology, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, Maryland, USA.
authors.forename: Haleyurgirisetty
authors.initials: H
authors.lastname: Mohan
authors.affiliation: Laboratory of Molecular Virology, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, Maryland, USA.
authors.forename: Jikun
authors.initials: J
authors.lastname: Liu
authors.affiliation: Center for Devices and Radiological Health, Food and Drug Administration, Silver Spring, Maryland, USA.
authors.forename: Haja Sittana
authors.initials: HS
authors.lastname: El Mubarak
authors.affiliation: Department of Laboratory Medicine, Yale University School of Medicine, New Haven, Connecticut, USA.
authors.forename: Marie L
authors.initials: ML
authors.lastname: Landry
authors.affiliation: Laboratory of Molecular Virology, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, Maryland, USA saivikram.vemula@fda.hhs.gov indira.hewlett@fda.hhs.gov.
authors.forename: Indira
authors.initials: I
authors.lastname: Hewlett
mesh_terms: D000956:Antigens, Viral / Q000032:analysis*; D003955:Diagnostic Tests, Routine / Q000379:methods*; D005063:Europium; D006801:Humans; D007118:Immunoassay / Q000379:methods; D009980:Influenza A virus / Q000302:isolation & purification*; D009981:Influenza B virus / Q000302:isolation & purification*; D007251:Influenza, Human / Q000175:diagnosis* / Q000821:virology; D053758:Nanoparticles; D012680:Sensitivity and Specificity
chemical_list: D000956:Antigens, Viral; D005063:Europium