title: Antimicrobial properties of Pseudomonas strains producing the antibiotic mupirocin.
abstract: Mupirocin is a polyketide antibiotic with broad antibacterial activity. It was isolated and characterized about 40 years ago from Pseudomonas fluorescens NCIMB 10586. To study the phylogenetic distribution of mupirocin producing strains in the genus Pseudomonas a large collection of Pseudomonas strains of worldwide origin, consisting of 117 Pseudomonas type strains and 461 strains isolated from different biological origins, was screened by PCR for the mmpD gene of the mupirocin gene cluster. Five mmpD(+) strains from different geographic and biological origin were identified. They all produced mupirocin and were strongly antagonistic against Staphylococcus aureus. Phylogenetic analysis showed that mupirocin production is limited to a single species. Inactivation of mupirocin production leads to complete loss of in vitro antagonism against S. aureus, except on certain iron-reduced media where the siderophore pyoverdine is responsible for the in vitro antagonism of a mupirocin-negative mutant. In addition to mupirocin some of the strains produced lipopeptides of the massetolide group. These lipopeptides do not play a role in the observed in vitro antagonism of the mupirocin producing strains against S. aureus.
authors.affiliation: Institut de Recherches Microbiologiques - Wiame, Campus du CERIA, 1 avenue Emile Gryson, bât 4B, B-1070 Bruxelles, Belgium. Electronic address: slmatthi@ulb.ac.be.
authors.forename: Sandra
authors.initials: S
authors.lastname: Matthijs
authors.affiliation: Institut de Recherches Microbiologiques - Wiame, Campus du CERIA, 1 avenue Emile Gryson, bât 4B, B-1070 Bruxelles, Belgium. Electronic address: cvdwauve@ulb.ac.be.
authors.forename: Corinne
authors.initials: C
authors.lastname: Vander Wauven
authors.affiliation: Institut de Recherches Microbiologiques - Wiame, Campus du CERIA, 1 avenue Emile Gryson, bât 4B, B-1070 Bruxelles, Belgium. Electronic address: Bertrand.Cornu@ulb.ac.be.
authors.forename: Bertrand
authors.initials: B
authors.lastname: Cornu
authors.affiliation: Department of Bioengineering Sciences, Research Group of Microbiology and Vlaams Instituut voor Biotechnologie, Vrije Universiteit Brussel, Brussels, Belgium. Electronic address: Lumeng.Ye@vub.ac.be.
authors.forename: Lumeng
authors.initials: L
authors.lastname: Ye
authors.affiliation: Department of Bioengineering Sciences, Research Group of Microbiology and Vlaams Instituut voor Biotechnologie, Vrije Universiteit Brussel, Brussels, Belgium. Electronic address: pcornel@vub.ac.ba.
authors.forename: Pierre
authors.initials: P
authors.lastname: Cornelis
authors.affiliation: School of Biosciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK. Electronic address: C.M.THOMAS@bham.ac.uk.
authors.forename: Christopher M
authors.initials: CM
authors.lastname: Thomas
authors.affiliation: Walloon Center for Industrial Biology, University of Liège, Gembloux Agro-Bio Tech, Gembloux, Belgium. Electronic address: marc.ongena@ulg.ac.be.
authors.forename: Marc
authors.initials: M
authors.lastname: Ongena
mesh_terms: D000900:Anti-Bacterial Agents / Q000378:metabolism / Q000494:pharmacology*; D000898:Antibiosis; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D005798:Genes, Bacterial; D055666:Lipopeptides / Q000378:metabolism / Q000494:pharmacology; D008969:Molecular Sequence Data; D016712:Mupirocin / Q000378:metabolism / Q000494:pharmacology*; D010802:Phylogeny; D011549:Pseudomonas / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism / Q000502:physiology*; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D013211:Staphylococcus aureus / Q000187:drug effects / Q000254:growth & development
chemical_list: D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D055666:Lipopeptides; D016712:Mupirocin
keywords: Massetolide; Mupirocin; Pseudomonas fluorescens NCIMB 10586; Pseudomonas sp. W2Aug9; Pyoverdine