title: Lentivirus-mediated silencing of SCIN inhibits proliferation of human lung carcinoma cells.
abstract: SCIN (scinderin) is a calcium-dependent actin severing and capping protein. Homologue in zebrafish has been found to be related with cell death. In the present study, we found that SCIN is highly expressed in human lung cancer specimens. However, the role of SCIN in lung cancer has not yet been determined. To investigate the function of SCIN in lung carcinoma cells, we took advantage of lentivirus-mediated RNA interference (RNAi) to knockdown SCIN expression in two lung carcinoma cell lines A549 and H1299. Silencing of SCIN significantly inhibited the proliferation and colony formation ability of both cell lines in vitro. Moreover, flow cytometry analysis showed that knockdown of SCIN led to G0/G1 phase cell cycle arrest as well as an excess accumulation of cells in the sub-G1 phase. Furthermore, depletion of SCIN resulted in a significant increase in Cyclin B1, p21 and PARP expression, and a little decrease in Cyclin D1 expression. These results suggest that SCIN plays an important role in lung carcinoma cell proliferation, and lentivirus-mediated silencing of SCIN might be a potential therapeutic approach for the treatment of lung cancer.
authors.affiliation: Department of Thoracic Surgery, The First Hospital, China Medical University, Shenyang 110001, China. Electronic address: hongxu_liu@163.com.
authors.forename: Hongxu
authors.initials: H
authors.lastname: Liu
authors.affiliation: Department of Thoracic Surgery, The First Hospital, China Medical University, Shenyang 110001, China.
authors.forename: Daiwang
authors.initials: D
authors.lastname: Shi
authors.affiliation: Department of Thoracic Surgery, The First Hospital, China Medical University, Shenyang 110001, China.
authors.forename: Tieqin
authors.initials: T
authors.lastname: Liu
authors.affiliation: Department of Thoracic Surgery, Liaoning Cancer Hospital & Institute, Shenyang 110042, China.
authors.forename: Zhanwu
authors.initials: Z
authors.lastname: Yu
authors.affiliation: Department of Thoracic Surgery, Benxi Central Hospital, Benxi 117000, China.
authors.forename: Chuanjiang
authors.initials: C
authors.lastname: Zhou
mesh_terms: D017209:Apoptosis; D002277:Carcinoma / Q000235:genetics* / Q000378:metabolism; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D056744:Cyclin B1 / Q000378:metabolism; D019938:Cyclin D1 / Q000378:metabolism; D050759:Cyclin-Dependent Kinase Inhibitor p21 / Q000378:metabolism; D018260:Gelsolin / Q000235:genetics*; D006801:Humans; D007150:Immunohistochemistry; D016086:Lentivirus / Q000235:genetics*; D008168:Lung / Q000378:metabolism; D008175:Lung Neoplasms / Q000235:genetics* / Q000378:metabolism; D011065:Poly(ADP-ribose) Polymerases / Q000378:metabolism; D034622:RNA Interference
chemical_list: C534699:CCNB1 protein, human; C530104:CCND1 protein, human; C493956:CDKN1A protein, human; D056744:Cyclin B1; D050759:Cyclin-Dependent Kinase Inhibitor p21; D018260:Gelsolin; C065395:scinderin; D019938:Cyclin D1; D011065:Poly(ADP-ribose) Polymerases
keywords: Lentivirus; Lung carcinoma; Proliferation; RNAi; SCIN