title: The measurement of protein synthesis for assessing proteostasis in studies of slowed aging.
abstract: Slowing the aging process can reduce the risk for multiple chronic diseases simultaneously. It is increasingly recognized that maintaining protein homeostasis (or proteostasis) is important for slowing the aging process. Since proteostasis is a dynamic process, monitoring it is not a simple task and requires use of appropriate methods. This review will introduce methods to assess protein and DNA synthesis using deuterium oxide (D2O), and how protein and DNA synthesis outcomes provide insight into proteostatic mechanisms. Finally, we provide a discussion on how these assessments of protein and DNA synthesis are "mechanistic" investigations and provide an appropriate framework for the further development of slowed aging treatments.
authors.affiliation: Department of Health and Exercise Science, Colorado State University, Fort Collins, CO, USA. Electronic address: Benjamin.f.miller@colostate.edu.
authors.forename: Benjamin F
authors.initials: BF
authors.lastname: Miller
authors.affiliation: Department of Health and Exercise Science, Colorado State University, Fort Collins, CO, USA.
authors.forename: Joshua C
authors.initials: JC
authors.lastname: Drake
authors.affiliation: Department of Chemistry and Biochemistry, Brigham Young University, Provo, UT, USA.
authors.forename: Bradley
authors.initials: B
authors.lastname: Naylor
authors.affiliation: Department of Chemistry and Biochemistry, Brigham Young University, Provo, UT, USA. Electronic address: jcprice@chem.byu.edu.
authors.forename: John C
authors.initials: JC
authors.lastname: Price
authors.affiliation: Department of Health and Exercise Science, Colorado State University, Fort Collins, CO, USA.
authors.forename: Karyn L
authors.initials: KL
authors.lastname: Hamilton
mesh_terms: D000367:Age Factors; D000375:Aging / Q000378:metabolism*; D000818:Animals; D031204:Caloric Restriction; D004261:DNA Replication; D017666:Deuterium Oxide / Q000378:metabolism; D006706:Homeostasis; D006801:Humans; D008098:Litter Size; D014176:Protein Biosynthesis / Q000187:drug effects; D011506:Proteins / Q000235:genetics / Q000378:metabolism*; D020123:Sirolimus / Q000494:pharmacology; D013997:Time Factors
chemical_list: D011506:Proteins; D017666:Deuterium Oxide; D020123:Sirolimus
keywords: Deuterium oxide; Long-lived model; Mitochondria; Proliferation; Stable isotope