title: Fluorescence-based ion-sensing with colloidal particles.
abstract: Particle-based fluorescence sensors for the quantification of specific ions can be made by coupling ion-sensitive fluorophores to carrier particles, or by using intrinsically fluorescent particles whose fluorescence properties depend on the concentration of the ions. Despite the advantages of such particle-based sensors for the quantitative detection of ions, such as the possibility to tune the surface chemistry and thus entry portal of the sensor particles to cells, they have also some associated problems. Problems involve for example crosstalk of the ion-sensitive fluorescence read-out with pH, or spectral overlap of the emission spectra of different fluorescent particles in multiplexing formats. Here the benefits of using particle-based fluorescence sensors, their limitations and strategies to overcome these limitations will be described and exemplified with selected examples.
authors.affiliation: Fachbereich Physik, Philipps Universität Marburg, Marburg, Germany.
authors.forename: Sumaira
authors.initials: S
authors.lastname: Ashraf
authors.affiliation: Fachbereich Physik, Philipps Universität Marburg, Marburg, Germany.
authors.forename: Carolina
authors.initials: C
authors.lastname: Carrillo-Carrion
authors.affiliation: Fachbereich Physik, Philipps Universität Marburg, Marburg, Germany.
authors.forename: Qian
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: Fachbereich Physik, Philipps Universität Marburg, Marburg, Germany.
authors.forename: Mahmoud G
authors.initials: MG
authors.lastname: Soliman
authors.affiliation: Fachbereich Physik, Philipps Universität Marburg, Marburg, Germany.
authors.forename: Raimo
authors.initials: R
authors.lastname: Hartmann
authors.affiliation: Fachbereich Physik, Philipps Universität Marburg, Marburg, Germany.
authors.forename: Beatriz
authors.initials: B
authors.lastname: Pelaz
authors.affiliation: CIC BiomaGUNE, San Sebastian, Spain.
authors.forename: Pablo
authors.initials: P
authors.lastname: Del Pino
authors.affiliation: Fachbereich Physik, Philipps Universität Marburg, Marburg, Germany; CIC BiomaGUNE, San Sebastian, Spain. Electronic address: wolfgang.parak@physik.uni-marburg.de.
authors.forename: Wolfgang J
authors.initials: WJ
authors.lastname: Parak
mesh_terms: D000818:Animals; D003102:Colloids / Q000494:pharmacology*; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000494:pharmacology*; D006863:Hydrogen-Ion Concentration; D007477:Ions; D035501:Uncertainty
chemical_list: D003102:Colloids; D005456:Fluorescent Dyes; D007477:Ions