title: Molecular characterization of porcine Siglec-10 and analysis of its expression in blood and tissues.
abstract: Siglecs are sialic acid binding Ig-like proteins involved in the control of leukocyte responses. In this study we describe the characterization of a porcine orthologue of Siglec-10. A cDNA clone was obtained from a porcine library which encodes a protein with sequence homology to human Siglec-10. This cDNA codes for a type I transmembrane protein containing four Ig-like domains, a transmembrane region, and a cytoplasmic tail with three tyrosine-based motifs, including a membrane-proximal Grb2-binding motif, and two ITIM motifs. When expressed on transfected cells, porcine Siglec-10 was able to bind red blood cells in a sialic acid-dependent manner. Monoclonal antibodies were developed against this protein and used to examine its cell and tissue distribution in the pig. Siglec-10 was found to be expressed on blood B cells and B cell areas of the spleen and lymph nodes. A weak expression was also detected on monocytes.
authors.affiliation: Dpto. de Biotecnología, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Ctra de la Coruña Km 7.5, 28040 Madrid, Spain.
authors.forename: Z
authors.initials: Z
authors.lastname: Escalona
authors.affiliation: Dpto. de Biotecnología, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Ctra de la Coruña Km 7.5, 28040 Madrid, Spain.
authors.forename: B
authors.initials: B
authors.lastname: Álvarez
authors.affiliation: National Institute of Agrobiological Sciences (NIAS), 2 Ikenodai, Tsukuba, Ibaraki 305-8602, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Uenishi
authors.affiliation: Institute of Japan Association for Techno-innovation in Agriculture, Forestry and Fisheries, 446-1 Ippaizuka, Kamiyokoba, Tsukuba, Ibaraki 305-0854, Japan.
authors.forename: D
authors.initials: D
authors.lastname: Toki
authors.affiliation: Dpto. de Biotecnología, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Ctra de la Coruña Km 7.5, 28040 Madrid, Spain.
authors.forename: M
authors.initials: M
authors.lastname: Yuste
authors.affiliation: Dpto. de Biotecnología, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Ctra de la Coruña Km 7.5, 28040 Madrid, Spain.
authors.forename: C
authors.initials: C
authors.lastname: Revilla
authors.affiliation: Dpto. de Biología Celular y de Inmunología, Facultad de Medicina, Universidad Complutense de Madrid, Avda. Complutense s/n, 28040 Madrid, Spain.
authors.forename: M Gómez
authors.initials: MG
authors.lastname: del Moral
authors.affiliation: Dpto. de Biotecnología, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Ctra de la Coruña Km 7.5, 28040 Madrid, Spain.
authors.forename: F
authors.initials: F
authors.lastname: Alonso
authors.affiliation: Dpto. de Biotecnología, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Ctra de la Coruña Km 7.5, 28040 Madrid, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Ezquerra
authors.affiliation: Dpto. de Biotecnología, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Ctra de la Coruña Km 7.5, 28040 Madrid, Spain. Electronic address: juncal@inia.es.
authors.forename: J
authors.initials: J
authors.lastname: Domínguez
mesh_terms: D016475:3T3 Cells; D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D001402:B-Lymphocytes / Q000378:metabolism*; D001483:Base Sequence; D016466:CHO Cells; D002460:Cell Line; D003412:Cricetulus; D004912:Erythrocytes / Q000378:metabolism*; D008198:Lymph Nodes / Q000378:metabolism; D051379:Mice; D009000:Monocytes / Q000378:metabolism; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D063265:Sialic Acid Binding Immunoglobulin-like Lectins / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology* / Q000378:metabolism*; D013154:Spleen / Q000378:metabolism; D013552:Swine / Q000235:genetics
chemical_list: D000911:Antibodies, Monoclonal; D063265:Sialic Acid Binding Immunoglobulin-like Lectins
keywords: B cells; Monoclonal antibody; Siglec-10; Swine