title: Effect of polydopamine on the biomimetic mineralization of mussel-inspired calcium phosphate cement in vitro.
abstract: Inspired by the excellent adhesive property of mussel adhesive protein, we added polydopamine (PDA) to calcium phosphate cement (PDA-CPC) to enhance its compressive strength previously. The mineralization and mechanism on PDA-CPC were investigated by soaking it in simulated body fluid in this study. The results indicated that PDA promoted the conversion of dicalcium phosphate dihydrate and α-tricalcium phosphate to hydroxyapatite (HA) in the early stage but inhibited this conversion subsequently. PDA promoted the rapid mineralization on PDA-CPC to form a layer of nanoscale calcium phosphate (CaP) whereas there was no CaP formation on the control-CPC after 1d of soaking. This layer of nanoscale CaP was similar to that of natural bone, which was always observed during soaking. X-ray photoelectron spectroscopy showed that the peak of CO of PDA existed in the newly formed CaP on PDA-CPC, indicating the co-precipitation of CaP with PDA. Furthermore, the newly formed CaP on PDA-CPC was HA confirmed by transmission electron microscopy, which the newly formed HA was in association with PDA. Therefore, PDA increased the capacity of mineralization of CPC and induced the formation of nanoscale bone-like apatite on PDA-CPC. Thus, this provides the feasible route for surface modification on CPC.
authors.affiliation: Key Lab of Advanced Technologies of Materials, Ministry of Education, School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu 610031, China.
authors.forename: Zongguang
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Key Lab of Advanced Technologies of Materials, Ministry of Education, School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu 610031, China. Electronic address: qushuxin@swjtu.edu.cn.
authors.forename: Shuxin
authors.initials: S
authors.lastname: Qu
authors.affiliation: Key Lab of Advanced Technologies of Materials, Ministry of Education, School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu 610031, China.
authors.forename: Xiaotong
authors.initials: X
authors.lastname: Zheng
authors.affiliation: Key Lab of Advanced Technologies of Materials, Ministry of Education, School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu 610031, China.
authors.forename: Xiong
authors.initials: X
authors.lastname: Xiong
authors.affiliation: Department of Plastic Surgery, Academy of Medical Sciences and Sichuan Provincial People's Hospital, Chengdu 610041, China.
authors.forename: Rong
authors.initials: R
authors.lastname: Fu
authors.affiliation: Department of Plastic Surgery, Academy of Medical Sciences and Sichuan Provincial People's Hospital, Chengdu 610041, China.
authors.forename: Kuangyun
authors.initials: K
authors.lastname: Tang
authors.affiliation: Department of Plastic Surgery, Academy of Medical Sciences and Sichuan Provincial People's Hospital, Chengdu 610041, China.
authors.forename: Zhendong
authors.initials: Z
authors.lastname: Zhong
authors.affiliation: Key Lab of Advanced Technologies of Materials, Ministry of Education, School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu 610031, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Weng
mesh_terms: D000818:Animals; D040761:Biomimetic Materials / Q000737:chemistry*; D049872:Bivalvia; D001843:Bone Cements / Q000138:chemical synthesis / Q000737:chemistry*; D002130:Calcium Phosphates / Q000138:chemical synthesis / Q000737:chemistry*; D019245:Compressive Strength; D017886:Durapatite / Q000737:chemistry; D007211:Indoles / Q000138:chemical synthesis / Q000737:chemistry*; D046529:Microscopy, Electron, Transmission; D049329:Nanostructures / Q000737:chemistry; D056951:Photoelectron Spectroscopy; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D014965:X-Rays
chemical_list: D001843:Bone Cements; D002130:Calcium Phosphates; D007211:Indoles; D011108:Polymers; C485828:alpha-tricalcium phosphate; C568283:polydopamine; D017886:Durapatite; C494366:calcium phosphate, dibasic, dihydrate
keywords: Biomimetic mineralization; Calcium phosphate cement; Polydopamine