title: Changes of major tea polyphenols and production of four new B-ring fission metabolites of catechins from post-fermented Jing-Wei Fu brick tea.
abstract: HPLC analysis of samples from four major fermentation procedures of Jing-Wei Fu brick tea showed that the level of major tea catechins epigallocatechin gallate (EGCG) and epicatechin gallate (ECG) dropped increasingly to about 1/3 in the final product. Phytochemical study of the final product led to the discovery of four new B-ring fission metabolites of catechins (BRFCs) Fuzhuanin C-F (1-4) together with three known BRFCs (5-7), six known catechins (8-13), five simple phenols (14-18), seven flavones and flavone glycosides (19-25), two alkaloids (26, 27), three triterpenoids (28-30) and one steroid (31). The structures were elucidated by spectroscopic methods including 1D and 2D NMR, LC-HR-ESI-MS, IR, and CD spectra. Five compounds (16-18, 28, 29) were reported for the first time in tea. Possible pathways for the degradation of major tea catechins and the generation of BRFCs were also provided.
authors.affiliation: Key Laboratory of Tea Biochemistry and Biotechnology, Anhui Agricultural University, 130 West Changjiang Road, Hefei City, Anhui Province 230036, China.
authors.forename: Yun-Fei
authors.initials: YF
authors.lastname: Zhu
authors.affiliation: Key Laboratory of Tea Biochemistry and Biotechnology, Anhui Agricultural University, 130 West Changjiang Road, Hefei City, Anhui Province 230036, China.
authors.forename: Jing-Jing
authors.initials: JJ
authors.lastname: Chen
authors.affiliation: Shaanxi Zhong Fu Tea Research Institute, Xianyang City, Shaanxi Province 712044, China.
authors.forename: Xiao-Ming
authors.initials: XM
authors.lastname: Ji
authors.affiliation: Shaanxi Zhong Fu Tea Research Institute, Xianyang City, Shaanxi Province 712044, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Hu
authors.affiliation: Key Laboratory of Tea Biochemistry and Biotechnology, Anhui Agricultural University, 130 West Changjiang Road, Hefei City, Anhui Province 230036, China.
authors.forename: Tie-Jun
authors.initials: TJ
authors.lastname: Ling
authors.affiliation: Key Laboratory of Tea Biochemistry and Biotechnology, Anhui Agricultural University, 130 West Changjiang Road, Hefei City, Anhui Province 230036, China.
authors.forename: Zheng-Zhu
authors.initials: ZZ
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Tea Biochemistry and Biotechnology, Anhui Agricultural University, 130 West Changjiang Road, Hefei City, Anhui Province 230036, China. Electronic address: baoguanhu@ahau.edu.cn.
authors.forename: Guan-Hu
authors.initials: GH
authors.lastname: Bao
authors.affiliation: Key Laboratory of Tea Biochemistry and Biotechnology, Anhui Agricultural University, 130 West Changjiang Road, Hefei City, Anhui Province 230036, China. Electronic address: xcwan@ahau.edu.cn.
authors.forename: Xiao-Chun
authors.initials: XC
authors.lastname: Wan
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005285:Fermentation; D010636:Phenols / Q000032:analysis*; D059808:Polyphenols / Q000032:analysis*; D013662:Tea / Q000737:chemistry*
chemical_list: D010636:Phenols; D059808:Polyphenols; D013662:Tea
keywords: (+)-Catechin (C, PubChem CID: 9064); (−)-Epiafzelechin (PubChem CID: 443639); (−)-Epicatechin (EC, PubChem CID: 72276); (−)-Epigallocatechin gallate (EGCG, PubChem CID: 65064); 2,5-Dihydroxy benzoic acid (PubChem CID: 3469); Aspergillus spp.; Astragalin (PubChem CID: 5282102); B-ring fission metabolites of catechins (BRFCs); Camellia sinensis; Canophyllol (PubChem CID: 623591); Epicatechin-3-O-(4′-O-methyl) gallate (PubChem CID: 21146794); Epicatechin-3-gallate (ECG, PubChem CID: 65056); Epigallocatechin (EGC, PubChem CID: 72277); Eurotium spp.; Fuzhuan brick tea (FBT); Fuzhuanin C–F; Gallic acid (GA, PubChem CID: 370); Gallicin (PubChem CID: 7428); Kaempferol (PubChem CID: 5280863); Myricetin (PubChem CID: 5281672); Nicotiflorin (PubChem CID: 5318767); Phloroglucinol (PubChem CID: 359); Pyrogallol (PubChem CID: 1057); Quercetin (PubChem CID: 5280343); Rutin (PubChem CID: 5280805); Theobromine (PubChem CID: 2519); Xanthocerin (PubChem CID: 5315332); α-Spinasterol (PubChem CID: 5281331)