title: Preparation of two flavonoid glycosides with unique structures from barley seedlings by membrane separation technology and preparative high-performance liquid chromatography.
abstract: Barley seedlings are rich in flavones that can have positive effects on people with antihypoxia and antifatigue. Lutonarin and saponarin are two major flavonoid glycosides that have unique structures in barley seedlings. This study presents a new approach for the preparation of lutonarin and saponarin from barely seedlings by membrane separation technology and preparative high-performance liquid chromatography. Preparative conditions of these two flavonoid glycosides by membrane separation technology were studied using response surface methodology. Under the optimized conditions, the total contents of these two flavonoid glycosides amounts to 17.0%.
authors.affiliation: Key Laboratory of Tibetan medicine Research, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, P.R. China.
authors.forename: Tao
authors.initials: T
authors.lastname: Chen
authors.forename: Hong-mei
authors.initials: HM
authors.lastname: Li
authors.forename: Deng-Lang
authors.initials: DL
authors.lastname: Zou
authors.forename: Yu-Zhi
authors.initials: YZ
authors.lastname: Du
authors.forename: Yu-Hu
authors.initials: YH
authors.lastname: Shen
authors.forename: Yulin
authors.initials: Y
authors.lastname: Li
mesh_terms: D047310:Apigenin / Q000138:chemical synthesis* / Q000737:chemistry / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid; D005419:Flavonoids / Q000138:chemical synthesis / Q000737:chemistry / Q000302:isolation & purification*; D005960:Glucosides / Q000138:chemical synthesis* / Q000737:chemistry / Q000302:isolation & purification*; D006027:Glycosides / Q000138:chemical synthesis / Q000737:chemistry / Q000302:isolation & purification*; D001467:Hordeum / Q000737:chemistry*; D015394:Molecular Structure; D036226:Seedlings / Q000737:chemistry*
chemical_list: D005419:Flavonoids; D005960:Glucosides; D006027:Glycosides; C000600695:lutonarin; C457771:saponarin; D047310:Apigenin
keywords: Barley seedlings; Lutonarin; Membrane separation technology; Response surface methodology; Saponarin