title: Mapping the accumulation of co-infiltrating CNS dendritic cells and encephalitogenic T cells during EAE.
abstract: Evidence from experimental autoimmune encephalomyelitis (EAE) suggests that CNS-infiltrating dendritic cells (DCs) are crucial for restimulation of coinfiltrating T cells. Here we systematically quantified and visualized the distribution and interaction of CNS DCs and T cells during EAE. We report marked periventricular accumulation of DCs and myelin-specific T cells during EAE disease onset prior to accumulation in the spinal cord, indicating that the choroid plexus-CSF axis is a CNS entry portal. Moreover, despite emphasis on spinal cord inflammation in EAE and in correspondence with MS pathology, inflammatory lesions containing interacting DCs and T cells are present in specific brain regions.
authors.affiliation: Department of Pathology and Laboratory Medicine, School of Medicine and Public Health, University of Wisconsin-Madison, Madison, WI 53706, United States; Graduate Training Programs of Cellular and Molecular Pathology, School of Medicine and Public Health, University of Wisconsin-Madison, Madison, WI 53706, United States.
authors.forename: Benjamin D
authors.initials: BD
authors.lastname: Clarkson
authors.affiliation: Department of Pathology and Laboratory Medicine, School of Medicine and Public Health, University of Wisconsin-Madison, Madison, WI 53706, United States.
authors.forename: Alec
authors.initials: A
authors.lastname: Walker
authors.affiliation: Department of Pathology and Laboratory Medicine, School of Medicine and Public Health, University of Wisconsin-Madison, Madison, WI 53706, United States.
authors.forename: Melissa
authors.initials: M
authors.lastname: Harris
authors.affiliation: Department of Neuroscience, School of Medicine and Public Health, University of Wisconsin-Madison, Madison, WI 53706, United States.
authors.forename: Aditya
authors.initials: A
authors.lastname: Rayasam
authors.affiliation: Department of Pathology and Laboratory Medicine, School of Medicine and Public Health, University of Wisconsin-Madison, Madison, WI 53706, United States.
authors.forename: Matyas
authors.initials: M
authors.lastname: Sandor
authors.affiliation: Department of Pathology and Laboratory Medicine, School of Medicine and Public Health, University of Wisconsin-Madison, Madison, WI 53706, United States. Electronic address: zfabry@facstaff.wisc.edu.
authors.forename: Zsuzsanna
authors.initials: Z
authors.lastname: Fabry
mesh_terms: D019264:Adoptive Transfer; D000704:Analysis of Variance; D000818:Animals; D001854:Bone Marrow Cells / Q000276:immunology / Q000378:metabolism; D039521:CD11c Antigen / Q000235:genetics / Q000378:metabolism; D002490:Central Nervous System / Q000276:immunology / Q000473:pathology*; D003713:Dendritic Cells / Q000276:immunology* / Q000473:pathology; D004195:Disease Models, Animal; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000139:chemically induced / Q000276:immunology* / Q000473:pathology*; D005434:Flow Cytometry; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D009154:Mutation / Q000235:genetics; D063308:Myelin-Oligodendrocyte Glycoprotein / Q000276:immunology / Q000633:toxicity; D010446:Peptide Fragments / Q000276:immunology / Q000633:toxicity; D013601:T-Lymphocytes / Q000276:immunology* / Q000473:pathology; D013997:Time Factors
chemical_list: D039521:CD11c Antigen; D008164:Luminescent Proteins; D063308:Myelin-Oligodendrocyte Glycoprotein; D010446:Peptide Fragments; C445081:myelin oligodendrocyte glycoprotein (35-55)
keywords: Choroid plexus; Dendritic cell; Experimental autoimmune encephalomyelitis; Multiple sclerosis; Periventricular; Rostral migratory stream