title: Preparation, characteristics and assessment of a novel gelatin-chitosan sponge scaffold as skin tissue engineering material.
abstract: In order to develop a skin tissue engineering material for wound dressing application, a novel gelatin-chitosan sponge scaffold was designed and studied. The effect of chitosan and gelatin ratio on the morphology, pore size, porosity, water uptake capacity, water retention capacity and the degradation behavior were evaluated. Biocompatibility was investigated by both MTT method and AO/EB staining method. Antibacterial assessment and in vivo pharmacodynamic was also studied to evaluate the potential for wound healing. Results showed the sponge scaffold have uniform porous structure with pore size range between 120 and 140 μm, high porosity (>90%), high water uptake capacity (>1500%), high water retention capacity (>400%), and degradation percent in 28 days between 38.3 and 53.9%. Biocompatibility results showed that the activity of cells could not be affected by the nature of the sponge and it was suitable for cell adhesion and proliferation for 21 days. In vivo evaluation indicated that the sponge scaffold could offer effective support and attachment to cells for skin wound healing. In conclusion, the developed sponge scaffold was a potential skin tissue engineering material with appropriate physical properties and good biocompatibility.
authors.affiliation: School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, People's Republic of China.
authors.forename: Fei
authors.initials: F
authors.lastname: Han
authors.affiliation: Wuxi PharmaTec Co., Ltd., Shanghai 200131, People's Republic of China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Dong
authors.affiliation: Jining Mental Health Hospital, Jining 272051, People's Republic of China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Su
authors.affiliation: School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, People's Republic of China.
authors.forename: Ran
authors.initials: R
authors.lastname: Yin
authors.affiliation: School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, People's Republic of China.
authors.forename: Aihua
authors.initials: A
authors.lastname: Song
authors.affiliation: School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, People's Republic of China. Electronic address: li_sanming@126.com.
authors.forename: Sanming
authors.initials: S
authors.lastname: Li
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D002448:Cell Adhesion / Q000502:physiology; D002460:Cell Line; D049109:Cell Proliferation / Q000502:physiology; D048271:Chitosan / Q000737:chemistry*; D005780:Gelatin / Q000737:chemistry*; D006801:Humans; D008297:Male; D016062:Porosity; D011817:Rabbits; D012867:Skin / Q000378:metabolism; D013997:Time Factors; D023822:Tissue Engineering; D054457:Tissue Scaffolds; D014867:Water / Q000378:metabolism; D014945:Wound Healing / Q000502:physiology
chemical_list: D001672:Biocompatible Materials; D014867:Water; D005780:Gelatin; D048271:Chitosan
keywords: Biocompatibility; Chitosan; Gelatin; Skin tissue engineering; Sponge scaffold