title: Structure of catalase determined by MicroED.
abstract: MicroED is a recently developed method that uses electron diffraction for structure determination from very small three-dimensional crystals of biological material. Previously we used a series of still diffraction patterns to determine the structure of lysozyme at 2.9 Å resolution with MicroED (Shi et al., 2013). Here we present the structure of bovine liver catalase determined from a single crystal at 3.2 Å resolution by MicroED. The data were collected by continuous rotation of the sample under constant exposure and were processed and refined using standard programs for X-ray crystallography. The ability of MicroED to determine the structure of bovine liver catalase, a protein that has long resisted atomic analysis by traditional electron crystallography, demonstrates the potential of this method for structure determination.
authors.affiliation: Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, United States.
authors.forename: Brent L
authors.initials: BL
authors.lastname: Nannenga
authors.affiliation: Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, United States.
authors.forename: Dan
authors.initials: D
authors.lastname: Shi
authors.affiliation: Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, United States.
authors.forename: Johan
authors.identifier: http://orcid.org/0000-0002-8936-0912
authors.initials: J
authors.lastname: Hattne
authors.affiliation: Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, United States.
authors.forename: Francis E
authors.initials: FE
authors.lastname: Reyes
authors.affiliation: Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, United States.
authors.forename: Tamir
authors.initials: T
authors.lastname: Gonen
mesh_terms: D000818:Animals; D002374:Catalase / Q000737:chemistry*; D002417:Cattle; D030562:Databases, Protein; D008958:Models, Molecular; D015203:Reproducibility of Results; D055672:Static Electricity; D014961:X-Ray Diffraction / Q000379:methods*
chemical_list: D002374:Catalase
keywords: MicroED; biochemistry; biophysics; catalase; cryo EM; electron diffraction; electron microscopy; micro crystals; none; structural biology