title: miR-155inhibitor reduces the proliferation and migration in osteosarcoma MG-63 cells.
abstract: As the most common malignant primary bone tumor in childhood, osteosarcoma (OS) maintains a high recurrence, despite the significant improvements in the overall survival rate of high-grade OS patients during the recent decades. Therefore, a novel therapy strategy is required for OS treatment. Recently, various microRNAs (miRNAs or miRs) have been confirmed as deregulated in OS, and themiR-155dysregulation in OS has been discovered by the microarray analysis. In the present study, the regulation ofmiR-155on the OS cell proliferation, migration and invasion on the MG-63 cells was exploredin vitro. ThemiR-155mimics were found to promote cell proliferation, colony formation, migration and invasion significantly, compared to the control miRNA. AnmiR-155inhibitor was also used to evaluate whethermiR-155served as a therapeutic target for OS. The results demonstrated that themiR-155inhibitor significantly reduced the proliferation, colony formation, migration and invasion of the MG-63 OS cells. Thus, the study confirmed the oncogenic regulation on the OS progression ofmiR-155, which could serve as a therapeutic target with anmiR-155inhibitor.
authors.affiliation: Department of Orthopaedics, The First Affiliated Hospital of Chongqing Medical University, Chongqing 404000, P.R. China ; Department of Orthopaedics, The Second Affiliated Hospital of Inner Mongolia Medical University, Hohhot, Inner Mongolia 010030, P.R. China.
authors.forename: Huicheng
authors.initials: H
authors.lastname: Lv
authors.affiliation: Department of Orthopaedics, The Second Affiliated Hospital of Inner Mongolia Medical University, Hohhot, Inner Mongolia 010030, P.R. China.
authors.forename: Jun
authors.initials: J
authors.lastname: Guo
authors.affiliation: Department of Ultrasound, The People's Hospital of Inner Mongolia, Hohhot, Inner Mongolia 010010, P.R. China.
authors.forename: Siqin
authors.initials: S
authors.lastname: Li
authors.affiliation: Department of Orthopaedics, The First Affiliated Hospital of Chongqing Medical University, Chongqing 404000, P.R. China.
authors.forename: Dianmin
authors.initials: D
authors.lastname: Jiang
keywords: miR-155 inhibitor; migration; osteosarcoma; proliferation