title: Peritoneal mesothelium promotes the progression of ovarian cancer cells in vitro and in a mice xenograft model in vivo.
abstract: The role of mesothelial cells in the intraperitoneal spread of ovarian cancer is still elusive. In particular, it is unclear whether these cells constitute a passive barrier preventing cancer cell progression or perhaps act as an active promoter of this process. In this report we show that omental human peritoneal mesothelial cells (HPMCs) stimulate adhesion and proliferation of ovarian cancer cells (A2780, OVCAR-3, SKOV-3). The latter was associated with the paracrine activity of GRO-1, IL-6, and IL-8 released to the environment by HPMCs. Furthermore, the growth dynamics of ovarian cancer xenografts produced in response to i.p. injection of ovarian cancer cells together with HPMCs was remarkably greater than for implantation of cancer cells alone. A layer of peritoneal mesothelium was consistently present in close proximity to the tumor mass in every xenograft model. In conclusion, our results indicate that HPMCs play a supporting role in the intraperitoneal invasiveness of ovarian malignancy, whose effect may be attributed to their ability to stimulate adhesion and proliferation of cancer cells.
authors.affiliation: Laboratory of Gerontology, Department of Pathophysiology, Poznań University of Medical Sciences, Rokietnicka 8 Str., 60-806 Poznań, Poland.
authors.forename: Justyna
authors.initials: J
authors.lastname: Mikuła-Pietrasik
authors.affiliation: Laboratory of Gerontology, Department of Pathophysiology, Poznań University of Medical Sciences, Rokietnicka 8 Str., 60-806 Poznań, Poland.
authors.forename: Patrycja
authors.initials: P
authors.lastname: Sosińska
authors.affiliation: Department of Toxicology, Poznań University of Medical Sciences, Dojazd 30 Str., 60-631 Poznań, Poland.
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Kucińska
authors.affiliation: Department of Toxicology, Poznań University of Medical Sciences, Dojazd 30 Str., 60-631 Poznań, Poland.
authors.forename: Marek
authors.initials: M
authors.lastname: Murias
authors.affiliation: Department of Clinical Pathology, Poznań University of Medical Sciences, Przybyszewskiego 49 Str., 60-355 Poznań, Poland.
authors.forename: Konstantin
authors.initials: K
authors.lastname: Maksin
authors.affiliation: Department of Histology and Embryology, Poznań University of Medical Sciences, Święcickiego 6 Str, 60-781 Poznań, Poland.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Malińska
authors.affiliation: Department of Histology and Embryology, Poznań University of Medical Sciences, Święcickiego 6 Str, 60-781 Poznań, Poland.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Ziółkowska
authors.affiliation: Department of Toxicology, Poznań University of Medical Sciences, Dojazd 30 Str., 60-631 Poznań, Poland.
authors.forename: Hanna
authors.initials: H
authors.lastname: Piotrowska
authors.affiliation: Department of Clinical Pathology, Poznań University of Medical Sciences, Przybyszewskiego 49 Str., 60-355 Poznań, Poland.
authors.forename: Aldona
authors.initials: A
authors.lastname: Woźniak
authors.affiliation: Laboratory of Gerontology, Department of Pathophysiology, Poznań University of Medical Sciences, Rokietnicka 8 Str., 60-806 Poznań, Poland. Electronic address: krzysztof.ksiazek@ump.edu.pl.
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Książek
mesh_terms: D000818:Animals; D002448:Cell Adhesion / Q000502:physiology; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000502:physiology; D054360:Chemokine CXCL1 / Q000378:metabolism; D018450:Disease Progression; D004848:Epithelium / Q000378:metabolism / Q000473:pathology*; D005260:Female; D064593:Heterografts; D006801:Humans; D015850:Interleukin-6 / Q000378:metabolism; D016209:Interleukin-8 / Q000378:metabolism; D051379:Mice; D010051:Ovarian Neoplasms / Q000378:metabolism / Q000473:pathology*; D010537:Peritoneum / Q000378:metabolism / Q000473:pathology*
chemical_list: C475829:CXCL1 protein, human; D054360:Chemokine CXCL1; D015850:Interleukin-6; D016209:Interleukin-8
keywords: Mice xenografts; Ovarian cancer metastasis; Peritoneal mesothelial cells