title: Negative methylation status of vimentin predicts improved prognosis in pancreatic carcinoma.
abstract: OBJECTIVE
  To determine the existence of a potential relationship between the methylation state of the Vimentin gene and its prognostic value in pancreatic cancer.
  
  
  METHODS
  Sixty-four primary tumor specimens and normal tissues were collected consecutively from pancreatic cancer patients during surgery at Hangzhou First People's Hospital and Affiliated Hospital of the Logistics University of the Chinese People's Armed Police Force. DNA was extracted from the samples and subsequently quantitative methylation-specific polymerase chain reaction was used to detect the Vimentin methylation status of the samples. All of the patients were followed up to December 2012. χ(2) test, Kaplan-Meier survival and Cox regression statistical models were used.
  
  
  RESULTS
  Out of 64 pancreatic cancer tissues, 21 were marked as Vimentin methylation-positive, and 43 were marked as Vimentin methylation-negative. The location of pancreatic carcinoma was related to the Vimentin methylation state. The pathological T staging (P < 0.001), adjuvant chemotherapy (P = 0.003) and the Vimentin methylation state (P = 0.037) were independent prognostic factors.
  
  
  CONCLUSIONS
  In our study, Vimentin methylation status can predict the prognosis of pancreatic cancer patients. However, additional experiments and clinical trials are needed to accurately validate this observation.
authors.affiliation: Yi-Feng Zhou, Xia Wang, Jing-Jing Xiang, Xiao-Feng Zhang, Department of Digestive Medicine, The First People's Hospital of Hangzhou, Hangzhou 310006, Zhejiang Province, China.
authors.forename: Yi-Feng
authors.initials: YF
authors.lastname: Zhou
authors.affiliation: Yi-Feng Zhou, Xia Wang, Jing-Jing Xiang, Xiao-Feng Zhang, Department of Digestive Medicine, The First People's Hospital of Hangzhou, Hangzhou 310006, Zhejiang Province, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Xu
authors.affiliation: Yi-Feng Zhou, Xia Wang, Jing-Jing Xiang, Xiao-Feng Zhang, Department of Digestive Medicine, The First People's Hospital of Hangzhou, Hangzhou 310006, Zhejiang Province, China.
authors.forename: Xia
authors.initials: X
authors.lastname: Wang
authors.affiliation: Yi-Feng Zhou, Xia Wang, Jing-Jing Xiang, Xiao-Feng Zhang, Department of Digestive Medicine, The First People's Hospital of Hangzhou, Hangzhou 310006, Zhejiang Province, China.
authors.forename: Jin-Shan
authors.initials: JS
authors.lastname: Sun
authors.affiliation: Yi-Feng Zhou, Xia Wang, Jing-Jing Xiang, Xiao-Feng Zhang, Department of Digestive Medicine, The First People's Hospital of Hangzhou, Hangzhou 310006, Zhejiang Province, China.
authors.forename: Jing-Jing
authors.initials: JJ
authors.lastname: Xiang
authors.affiliation: Yi-Feng Zhou, Xia Wang, Jing-Jing Xiang, Xiao-Feng Zhang, Department of Digestive Medicine, The First People's Hospital of Hangzhou, Hangzhou 310006, Zhejiang Province, China.
authors.forename: Zhao-Shen
authors.initials: ZS
authors.lastname: Li
authors.affiliation: Yi-Feng Zhou, Xia Wang, Jing-Jing Xiang, Xiao-Feng Zhang, Department of Digestive Medicine, The First People's Hospital of Hangzhou, Hangzhou 310006, Zhejiang Province, China.
authors.forename: Xiao-Feng
authors.initials: XF
authors.lastname: Zhang
mesh_terms: D014408:Biomarkers, Tumor / Q000235:genetics*; D002277:Carcinoma / Q000235:genetics* / Q000401:mortality / Q000473:pathology / Q000628:therapy; D017024:Chemotherapy, Adjuvant; D019175:DNA Methylation; D005260:Female; D006801:Humans; D053208:Kaplan-Meier Estimate; D008297:Male; D009367:Neoplasm Staging; D010190:Pancreatic Neoplasms / Q000235:genetics* / Q000401:mortality / Q000473:pathology / Q000628:therapy; D016133:Polymerase Chain Reaction; D011237:Predictive Value of Tests; D016016:Proportional Hazards Models; D013997:Time Factors; D016896:Treatment Outcome; D014746:Vimentin / Q000235:genetics*
chemical_list: D014408:Biomarkers, Tumor; D014746:Vimentin
keywords: Methylation; Pancreatic carcinoma; Prognosis; Vimentin