title: Reversible Ca(2+) switch of an engineered allosteric antioxidant selenoenzyme.
abstract: A Ca(2+) -responsive artificial selenoenzyme was constructed by computational design and engineering of recoverin with the active center of glutathione peroxidase (GPx). By combining the recognition capacity for the glutathione (GSH) substrate and the steric orientation of the catalytic selenium moiety, the engineered selenium-containing recoverin exhibits high GPx activity for the catalyzed reduction of H2 O2 by glutathione (GSH). Moreover, the engineered selenoenzyme can be switched on/off by Ca(2+) -induced allosterism of the protein recoverin. This artificial selenoenzyme also displays excellent antioxidant ability when it was evaluated using a mitochondrial oxidative damage model, showing great potential for controlled catalysis in biomedical applications.
authors.affiliation: State Key Laboratory of Supramolecular Structure and Materials, College of Chemistry, Jilin University, 2699 Qianjin Road, Changchun 130012 ( P.R. China).
authors.forename: Chunqiu
authors.initials: C
authors.lastname: Zhang
authors.forename: Tiezheng
authors.initials: T
authors.lastname: Pan
authors.forename: Christian
authors.initials: C
authors.lastname: Salesse
authors.forename: Dongmei
authors.initials: D
authors.lastname: Zhang
authors.forename: Lu
authors.initials: L
authors.lastname: Miao
authors.forename: Liang
authors.initials: L
authors.lastname: Wang
authors.forename: Yuzhou
authors.initials: Y
authors.lastname: Gao
authors.forename: Jiayun
authors.initials: J
authors.lastname: Xu
authors.forename: Zeyuan
authors.initials: Z
authors.lastname: Dong
authors.forename: Quan
authors.initials: Q
authors.lastname: Luo
authors.forename: Junqiu
authors.initials: J
authors.lastname: Liu
mesh_terms: D000975:Antioxidants / Q000737:chemistry* / Q000494:pharmacology; D001665:Binding Sites; D002118:Calcium / Q000737:chemistry*; D002384:Catalysis; D005979:Glutathione Peroxidase / Q000737:chemistry*; D006861:Hydrogen Peroxide / Q000737:chemistry; D051603:Recoverin / Q000737:chemistry*; D012643:Selenium / Q000737:chemistry; D017279:Selenocysteine / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D017279:Selenocysteine; D051603:Recoverin; D006861:Hydrogen Peroxide; D005979:Glutathione Peroxidase; D012643:Selenium; D002118:Calcium
keywords: antioxidants; artificial glutathione peroxidase; cysteine-auxotrophic system; recoverin; selenocysteine