title: Upregulation of microRNA processing enzymes Drosha and Dicer in gestational diabetes mellitus.
abstract: MicroRNAs (miRNAs) have been shown to play important roles in diverse cellular processes and linked to variety of disorders. Dicer and Drosha are two major enzymes in the miRNA biogenesis process. DGCR8 is the assistant of Drosha in the microprocessor complex. In this study, we evaluated the mRNA expression profiles of major miRNA processing machinery Drosha, Dicer, and DGCR8 in gestational diabetes mellitus (GDM), pregnant and healthy women. Our findings indicate that the expression levels of Drosha, Dicer and DGCR8 were upregulated in both pregnant and GDM patients compared to the control group. However, Drosha and Dicer were upregulated more than pregnant group. In conclusion, we detected dysregulation of Drosha, Dicer and DGCR8 expression in pregnant and GDM patients when compared to healthy control participants. Therefore, we favor the hypothesis that miRNAs are involved in the development of GDM.
authors.affiliation: Department of Obstetrics and Gynecology .
authors.forename: Giti
authors.initials: G
authors.lastname: Rahimi
authors.forename: Naser
authors.initials: N
authors.lastname: Jafari
authors.forename: Mehnoush
authors.initials: M
authors.lastname: Khodabakhsh
authors.forename: Zohreh
authors.initials: Z
authors.lastname: Shirzad
authors.forename: Hadi Peeri
authors.initials: HP
authors.lastname: Dogaheh
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D053487:DEAD-box RNA Helicases / Q000235:genetics*; D016640:Diabetes, Gestational / Q000235:genetics* / Q000378:metabolism; D005260:Female; D015971:Gene Expression Regulation, Enzymologic; D006801:Humans; D035683:MicroRNAs / Q000378:metabolism*; D011247:Pregnancy; D012323:RNA Processing, Post-Transcriptional; D016601:RNA-Binding Proteins / Q000235:genetics; D043244:Ribonuclease III / Q000235:genetics*; D015854:Up-Regulation; D055815:Young Adult
chemical_list: C474043:DGCR8 protein, human; D035683:MicroRNAs; D016601:RNA-Binding Proteins; C408087:DICER1 protein, human; C517918:DROSHA protein, human; D043244:Ribonuclease III; D053487:DEAD-box RNA Helicases
keywords: DGCR8; Dicer; Drosha; Gestational Diabetes Mellitus; microRNA; pregnancy