title: A soybean C2H2-type zinc finger gene GmZF1 enhanced cold tolerance in transgenic Arabidopsis.
abstract: Zinc finger proteins were involved in response to different environmental stresses in plant species. A typical Cys2/His2-type (C2H2-type) zinc finger gene GmZF1 from soybean was isolated and was composed of 172 amino acids containing two conserved C2H2-type zinc finger domains. Phylogenetic analysis showed that GmZF1 was clustered on the same branch with six C2H2-type ZFPs from dicotyledonous plants excepting for GsZFP1, and distinguished those from monocotyledon species. The GmZF1 protein was localized at the nucleus, and has specific binding activity with EP1S core sequence, and nucleotide mutation in the core sequence of EPSPS promoter changed the binding ability between GmZF1 protein and core DNA element, implying that two amino acid residues, G and C boxed in core sequence TGACAGTGTCA possibly play positive regulation role in recognizing DNA-binding sites in GmZF1 proteins. High accumulation of GmZF1 mRNA induced by exogenous ABA suggested that GmZF1 was involved in an ABA-dependent signal transduction pathway. Over-expression of GmZF1 significantly improved the contents of proline and soluble sugar and decreased the MDA contents in the transgenic lines exposed to cold stress, indicating that transgenic Arabidopsis carrying GmZF1 gene have adaptive mechanisms to cold stress. Over-expression of GmZF1 also increased the expression of cold-regulated cor6.6 gene by probably recognizing protein-DNA binding sites, suggesting that GmZF1 from soybean could enhance the tolerance of Arabidopsis to cold stress by regulating expression of cold-regulation gene in the transgenic Arabidopsis.
authors.affiliation: Key Lab. of Plant Nutrition and Fertilizer, Ministry of Agriculture, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing, China.
authors.forename: Guo-Hong
authors.initials: GH
authors.lastname: Yu
authors.affiliation: Key Lab. of Plant Nutrition and Fertilizer, Ministry of Agriculture, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing, China.
authors.forename: Lin-Lin
authors.initials: LL
authors.lastname: Jiang
authors.affiliation: Key Lab. of Plant Nutrition and Fertilizer, Ministry of Agriculture, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing, China; Institute of Agro-Products Processing Science and Technology, Chinese Academy of Agricultural Sciences, Beijing, China.
authors.forename: Xue-Feng
authors.initials: XF
authors.lastname: Ma
authors.affiliation: Institute of Crop Science, Chinese Academy of Agricultural Sciences (CAAS)/National Key Facility for Crop Gene Resources and Genetic Improvement, Key Laboratory of Biology and Genetic Improvement of Triticeae Crops, Ministry of Agriculture, Beijing, China.
authors.forename: Zhao-Shi
authors.initials: ZS
authors.lastname: Xu
authors.affiliation: Key Lab. of Plant Nutrition and Fertilizer, Ministry of Agriculture, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing, China.
authors.forename: Meng-Meng
authors.initials: MM
authors.lastname: Liu
authors.affiliation: Key Lab. of Plant Nutrition and Fertilizer, Ministry of Agriculture, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing, China.
authors.forename: Shu-Guang
authors.initials: SG
authors.lastname: Shan
authors.affiliation: Key Lab. of Plant Nutrition and Fertilizer, Ministry of Agriculture, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing, China.
authors.forename: Xian-Guo
authors.initials: XG
authors.lastname: Cheng
mesh_terms: D000040:Abscisic Acid / Q000494:pharmacology; D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000166:cytology / Q000187:drug effects / Q000235:genetics* / Q000502:physiology*; D058639:Cold-Shock Response / Q000187:drug effects / Q000235:genetics*; D004247:DNA / Q000378:metabolism; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D006720:Homozygote; D042541:Intracellular Space / Q000187:drug effects / Q000378:metabolism; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D030821:Plants, Genetically Modified; D021381:Protein Transport / Q000187:drug effects; D013025:Glycine max / Q000235:genetics*; D014170:Transformation, Genetic; D016335:Zinc Fingers
chemical_list: D010940:Plant Proteins; D000040:Abscisic Acid; D004247:DNA