title: Peptide and peptide nucleic acid syntheses using a DNA/RNA synthesizer.
abstract: The use of an ABI 394 DNA/RNA synthesizer for peptide and peptide nucleic acid (PNA) syntheses is described. No additional physical part or software is needed for the application. A commercially available large DNA synthesis column was used, and only about half of its volume was filled with resin when the resin was fully swollen. With additional space in the top portion of the column, agitation of reaction mixture was achieved by bubbling argon from the bottom without losing solution. Removing solutions from column was achieved by flushing argon from top to bottom. Two peptide and two PNA sequences were synthesized. Good yields were obtained in all the cases. The method is easy to follow by researchers who are familiar with DNA/RNA synthesizer.
authors.affiliation: Department of Chemistry, Michigan Technological University, 1400 Townsend Drive, Houghton, MI, 49931.
authors.forename: Durga
authors.initials: D
authors.lastname: Pokharel
authors.forename: Suntara
authors.initials: S
authors.lastname: Fueangfung
authors.forename: Mingcui
authors.initials: M
authors.lastname: Zhang
authors.forename: Shiyue
authors.initials: S
authors.lastname: Fang
mesh_terms: D002851:Chromatography, High Pressure Liquid; D056148:Chromatography, Reverse-Phase; D004247:DNA / Q000737:chemistry*; D020135:Peptide Nucleic Acids / Q000138:chemical synthesis* / Q000737:chemistry; D010455:Peptides / Q000138:chemical synthesis* / Q000737:chemistry; D012313:RNA / Q000737:chemistry*; D013997:Time Factors
chemical_list: D020135:Peptide Nucleic Acids; D010455:Peptides; D012313:RNA; D004247:DNA
keywords: DNA; RNA; peptide; peptide nucleic acid; solid phase synthesis; synthesizer