title: TIMP1 in conditioned media of human adipose stromal cells protects neurons against oxygen-glucose deprivation injury.
abstract: Adipose stromal cells (ASC) can protect neurons when administered to brains due to secreted trophic factors. Our previous studies demonstrated that several neurotrophic factors such as brain-derived neurotropic factor (BDNF) and insulin like growth factor-1 (IGF-1) in ASC conditioned media (ASC-CM) can protect brains against hypoxic-ischemic (HI) injury in neonatal rats. In this study, we demonstrated that human ASC-CM potently blockeds caspase-3 mediated cortical neuronal apoptosis under in vitro oxygen-glucose deprivation (OGD). Interestingly, tissue inhibitor of metalloproteinase 1 (TIMP1), a non neurotrophic factor, played a significant role in the ASC-CM-induced neural protection against OGD. Thus, this study establishes the therapeutic potential of TIMP1 together with other neurotrophic factors in ASC-CM for treating cerebral HI disorders.
authors.affiliation: General Hospital of the Chinese People's Armed Police Forces, Beijing, PR China.
authors.forename: Shiwei
authors.initials: S
authors.lastname: Du
authors.affiliation: General Hospital of the Chinese People's Armed Police Forces, Beijing, PR China. Electronic address: neurology@163.com.
authors.forename: Gengsheng
authors.initials: G
authors.lastname: Mao
authors.affiliation: Department of Neurology, School of Medicine, Indiana University, Indianapolis, IN 46202, United States.
authors.forename: Timothy
authors.initials: T
authors.lastname: Zhu
authors.affiliation: Department of Pediatrics, Navy General Hospital, No. 6 Fucheng Road, Beijing, PR China.
authors.forename: Zuo
authors.initials: Z
authors.lastname: Luan
authors.affiliation: Department of Neurology, School of Medicine, Indiana University, Indianapolis, IN 46202, United States.
authors.forename: Yansheng
authors.initials: Y
authors.lastname: Du
authors.affiliation: Department of Neurology, School of Medicine, Indiana University, Indianapolis, IN 46202, United States. Electronic address: huiygu@iupui.edu.
authors.forename: Huiying
authors.initials: H
authors.lastname: Gu
mesh_terms: D000273:Adipose Tissue / Q000166:cytology / Q000378:metabolism*; D000818:Animals; D017209:Apoptosis; D053148:Caspase 3 / Q000378:metabolism; D002470:Cell Survival; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology; D017077:Culture Media, Conditioned; D004789:Enzyme Activation; D005947:Glucose / Q000378:metabolism*; D006801:Humans; D009474:Neurons / Q000166:cytology*; D010100:Oxygen / Q000378:metabolism*; D017207:Rats, Sprague-Dawley; D017154:Stromal Cells / Q000378:metabolism; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000378:metabolism*
chemical_list: D017077:Culture Media, Conditioned; C560822:TIMP1 protein, human; D019715:Tissue Inhibitor of Metalloproteinase-1; D053148:Caspase 3; D005947:Glucose; D010100:Oxygen
keywords: Adipose stromal cells; Oxygen-glucose deprivation; TIMP1