title: Effects of a nutrient additive on the density of functional bacteria and the microbial community structure of bioorganic fertilizer.
abstract: In this study, a secondary composting experiment was conducted to investigate the effects of concentrated monosodium glutamate wastewater (CMGW) as a nutrient additive on enhancing the density of functional bacteria in bioorganic fertilizer (BOF). The results showed that, as the CMGW was added, the composting mixture temperature rose more quickly and strongly, and the functional bacteria Bacillus subtilis F2 percentage in spores increased significantly. Furthermore, both the highest species richness value (Rs) of a given sample and similarity coefficient value (Cs) between a sample and strain F2 based on DGGE analysis were also observed in the treatment with CMGW. A similar effect of CMGW on the microbial community structure was verified by means of illumine-MiSeq sequencing. It may thus be concluded that CMGW is a recommendable nutrient additive for F2 predominating in the secondary composting process.
authors.affiliation: Ministry of Education Key Laboratory of Environmental Remediation and Ecological Health, College of Environmental and Resource Sciences, Zhejiang University, Hangzhou 310058, PR China.
authors.forename: Li
authors.initials: L
authors.lastname: Liu
authors.affiliation: Zhejiang Provincial Key Laboratory of Subtropical Soil Science and Plant Nutrition, College of Environmental and Resource Sciences, Zhejiang University, Hangzhou 310058, PR China.
authors.forename: Tianyu
authors.initials: T
authors.lastname: Li
authors.affiliation: Zhejiang Provincial Key Laboratory of Subtropical Soil Science and Plant Nutrition, College of Environmental and Resource Sciences, Zhejiang University, Hangzhou 310058, PR China.
authors.forename: Xinghua
authors.initials: X
authors.lastname: Wei
authors.affiliation: Fengyuan Agricultural Science and Technology Co., Ltd., Jinhua 321000, PR China.
authors.forename: Bokang
authors.initials: B
authors.lastname: Jiang
authors.affiliation: Ministry of Education Key Laboratory of Environmental Remediation and Ecological Health, College of Environmental and Resource Sciences, Zhejiang University, Hangzhou 310058, PR China; Zhejiang Provincial Key Laboratory of Subtropical Soil Science and Plant Nutrition, College of Environmental and Resource Sciences, Zhejiang University, Hangzhou 310058, PR China. Electronic address: pfang@zju.edu.cn.
authors.forename: Ping
authors.initials: P
authors.lastname: Fang
mesh_terms: D001412:Bacillus subtilis / Q000254:growth & development* / Q000302:isolation & purification; D018929:Cell Culture Techniques / Q000379:methods*; D015169:Colony Count, Microbial; D005308:Fertilizers / Q000382:microbiology*; D059013:Microbial Consortia / Q000502:physiology*; D012987:Soil / Q000737:chemistry*; D012988:Soil Microbiology; D062065:Wastewater / Q000382:microbiology*
chemical_list: D005308:Fertilizers; D012987:Soil; D062065:Wastewater
keywords: Bacillus subtilis F2; Bioorganic fertilizer (BOF); Concentrated monosodium glutamate wastewater (CMGW); Illumine-MiSeq; PCR-DGGE