title: Knockout of MIMP protein in lactobacillus plantarum lost its regulation of intestinal permeability on NCM460 epithelial cells through the zonulin pathway.
abstract: BACKGROUND
  Previous studies indicated that the micro integral membrane protein located within the media place of the integral membrane protein of Lactobacillus plantarum CGMCC 1258 had protective effects against the intestinal epithelial injury. In our study, we mean to establish micro integral membrane protein -knockout Lactobacillus plantarum (LPKM) to investigate the change of its protective effects and verify the role of micro integral membrane protein on protection of normal intestinal barrier function.
  
  
  METHODS
  Binding assay and intestinal permeability were performed to verify the protective effects of micro integral membrane protein on intestinal permeability in vitro and in vivo. Molecular mechanism was also determined as the zonulin pathway. Clinical data were also collected for further verification of relationship between zonulin level and postoperative septicemia.
  
  
  RESULTS
  LPKM got decreased inhibition of EPEC adhesion to NCM460 cells. LPKM had lower ability to alleviate the decrease of intestinal permeability induced by enteropathogenic-e.coli, and prevent enteropathogenic-e.coli -induced increase of zonulin expression. Overexpression of zonulin lowered the intestinal permeability regulated by Lactobacillus plantarum. There was a positive correlation between zonulin level and postoperative septicemia. Therefore, micro integral membrane protein could be necessary for the protective effects of Lactobacillus plantarum on intestinal barrier.
  
  
  CONCLUSIONS
  MIMP might be a positive factor for Lactobacillus plantarum to protect the intestinal epithelial cells from injury, which could be related to the zonulin pathway.
authors.affiliation: Department of Colorectal Surgery, Gastrointestinal Institute of Sun Yat-Sen University, the Sixth Affiliated Hospital of Sun Yat-Sen University (Guangdong Gastrointestinal Hospital), 26 Yuancun Erheng Road, Guangzhou, Guangdong 510655, China. liuzhh26@mail.sysu.edu.cn.
authors.forename: Zhihua
authors.initials: Z
authors.lastname: Liu
authors.forename: Liang
authors.initials: L
authors.lastname: Kang
authors.forename: Chao
authors.initials: C
authors.lastname: Li
authors.forename: Chao
authors.initials: C
authors.lastname: Tong
authors.forename: Meijin
authors.initials: M
authors.lastname: Huang
authors.forename: Xingwei
authors.initials: X
authors.lastname: Zhang
authors.forename: Nanqi
authors.initials: N
authors.lastname: Huang
authors.forename: Mary Pat
authors.initials: MP
authors.lastname: Moyer
authors.forename: Huanlong
authors.initials: H
authors.lastname: Qin
authors.forename: Jianping
authors.initials: J
authors.lastname: Wang
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000378:metabolism*; D002478:Cells, Cultured; D002772:Cholera Toxin / Q000378:metabolism*; D004847:Epithelial Cells; D006242:Haptoglobins; D007413:Intestinal Mucosa / Q000378:metabolism*; D048191:Lactiplantibacillus plantarum / Q000378:metabolism*; D008565:Membrane Proteins / Q000378:metabolism*; D051379:Mice; D018345:Mice, Knockout; D010539:Permeability; D011498:Protein Precursors
chemical_list: D001426:Bacterial Proteins; D006242:Haptoglobins; D008565:Membrane Proteins; D011498:Protein Precursors; C408043:zonulin; D002772:Cholera Toxin