title: Effect of the physicochemical properties of pure or chitosan-coated poly(L-lactic acid)scaffolds on the chondrogenic differentiation of mesenchymal stem cells from osteoarthritic patients.
abstract: Due to the attractive properties of poly(L-lactic acid) (PLLA) and chitosan (CHT) for tissue engineering applications, this study is aimed at analyzing the chondrogenic potential of human bone marrow-derived mesenchymal stem cells (BM-MSCs) derived from osteoarthritic (OA) patients, in pure or CHT-coated PLLA, using different coating methodologies. Although PLLA scaffolds coated in one-step (PLLA-CHT1) yielded CHT smooth pellicles filling the PLLA macropores, a two-step strategy resulted in a CHT fiber-like thin coating covering PLLA pore walls (PLLA-CHT2). Both strategies led to the incorporation of similar content of CHT and a two-fold increase in the scaffold's water uptake capacity, providing elastic moduli values comparable to the ones found for human articular cartilage. After confirming OA-derived BM-MSCs, metabolic activity in the scaffolds, the chondrogenic potential was tested at 30 and 60 days, in a chondrogenic differentiation medium. PLLA scaffolds improved the chondrogenic differentiation of BM-MSCs, regarding cell pellet conventional culture and presented a typical cartilage zonal distribution, although was not able to revert a terminal differentiation. In PLLA-CHT1, on a short term, a rather heterogeneous tissue was formed, with confined areas of either slower cell infiltration or a faster maturation, with enhanced chondrogenic phenotype. In PLLA-CHT2, a similar tissue to PLLA was obtained, albeit on the long term, these scaffolds helped to maintain a hyaline-like phenotype and prevented the advance of the hypertrophic process. These results demonstrate the importance of the scaffolds microenvironment on the cellular events of chondrogenesis.
authors.affiliation: 1 Biomedical Research Networking Center in Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN) , Spain .
authors.forename: Joana
authors.initials: J
authors.lastname: Magalhães
authors.forename: Myriam
authors.initials: M
authors.lastname: Lebourg
authors.forename: Harmony
authors.initials: H
authors.lastname: Deplaine
authors.forename: José Luis
authors.initials: JL
authors.lastname: Gómez Ribelles
authors.forename: Francisco J
authors.initials: FJ
authors.lastname: Blanco
mesh_terms: D000327:Adsorption; D002454:Cell Differentiation; D049109:Cell Proliferation; D002470:Cell Survival; D060833:Cellular Microenvironment; D048271:Chitosan / Q000737:chemistry*; D019902:Chondrocytes / Q000473:pathology*; D020219:Chondrogenesis / Q000502:physiology; D020099:Coated Materials, Biocompatible / Q000138:chemical synthesis; D006801:Humans; D019344:Lactic Acid / Q000737:chemistry*; D059630:Mesenchymal Stem Cells / Q000473:pathology*; D010003:Osteoarthritis / Q000473:pathology*; D011091:Polyesters; D011108:Polymers / Q000737:chemistry*; D054457:Tissue Scaffolds
chemical_list: D020099:Coated Materials, Biocompatible; D011091:Polyesters; D011108:Polymers; D019344:Lactic Acid; C033616:poly(lactide); D048271:Chitosan