title: Effect of citric acid crosslinking cellulose-based hydrogels on osteogenic differentiation.
abstract: Understanding the relationships between material surface properties and cellular responses is essential to designing optimal material surfaces for implantation and tissue engineering. In this study, cellulose hydrogels were crosslinked using a non-toxic and natural component namely citric acid. The chemical treatment induces COOH functional groups that improve the hydrophilicity, roughness, and materials rheological properties. The physiochemical, morphological, and mechanical analyses were performed to analyze the material surface before and after crosslinking. This approach would help determine if the effect of chemical treatment on cellulose hydrogel improves the hydrophilicity, roughness, and rheological properties of the scaffold. In this study, it was demonstrated that the biological responses of human mesenchymal stem cell with regard to cell adhesion, proliferation, and differentiation were influenced in vitro by changing the surface chemistry and roughness.
authors.affiliation: Institute of Polymers, Composites and Biomaterials (IPCB), National Research Council of Italy, Mostra d'Oltremare Pad.20, Viale Kennedy 54, Naples, 80125, Italy.
authors.forename: M G
authors.initials: MG
authors.lastname: Raucci
authors.forename: M A
authors.initials: MA
authors.lastname: Alvarez-Perez
authors.forename: C
authors.initials: C
authors.lastname: Demitri
authors.forename: D
authors.initials: D
authors.lastname: Giugliano
authors.forename: V
authors.initials: V
authors.lastname: De Benedictis
authors.forename: A
authors.initials: A
authors.lastname: Sannino
authors.forename: L
authors.initials: L
authors.lastname: Ambrosio
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D015415:Biomarkers / Q000378:metabolism; D002448:Cell Adhesion / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects*; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D002482:Cellulose / Q000737:chemistry / Q000494:pharmacology; D019343:Citric Acid / Q000494:pharmacology*; D003432:Cross-Linking Reagents / Q000494:pharmacology*; D006801:Humans; D020100:Hydrogels / Q000494:pharmacology*; D057927:Hydrophobic and Hydrophilic Interactions; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D018625:Microscopy, Atomic Force; D010012:Osteogenesis / Q000187:drug effects*; D012212:Rheology; D017550:Spectroscopy, Fourier Transform Infrared; D014158:Transcription, Genetic / Q000187:drug effects; D014783:Viscosity; D014867:Water / Q000737:chemistry
chemical_list: D015415:Biomarkers; D003432:Cross-Linking Reagents; D020100:Hydrogels; D014867:Water; D019343:Citric Acid; D002482:Cellulose; D000469:Alkaline Phosphatase
keywords: human mesenchymal stem cells; hydrogels; natural polymers; osteogenic differentiation; surface properties