title: Metabolic engineering of Arabidopsis for remediation of different polycyclic aromatic hydrocarbons using a hybrid bacterial dioxygenase complex.
abstract: The widespread presence of polycyclic aromatic hydrocarbons (PAHs) and their potential harm to various organisms has generated interest in efficiently eliminating these compounds from the environment. Phytoremediation is an efficient technology for cleaning up pollutants. However, unlike microorganisms, plants lack the catabolic pathway for complete degradation of these dangerous groups of compounds. One way to enhance the potential of plants for remediation of these compounds is by transferring genes involved in xenobiotic degradation from microbes to plants. In this paper, four genes, namely nidA and nidB (encoding the large and small subunits of naphthalene dioxygenase of Mycobacterium vanbaalenii PYR-1) as well as NahAa and NahAb (encoding flavoprotein reductase and ferredoxin of the electron-transport chain of the Pseudomonas putida G7 naphthalene dioxygenase system), were transferred and ectopically expressed in Arabidopsis thaliana. Transgenic Arabidopsis plants overexpressing the heterozygous naphthalene dioxygenase system exhibited enhanced tolerance toward 2-4 rings PAHs. Transgenic plants assimilated PAHs from the culture media faster and accumulated less in vivo than wild-type plants. Furthermore, examination of metabolic intermediates by gas chromatography-mass spectrometry revealed that the naphthalene metabolic pathway in transgenic plants mainly involves the dioxygenase pathway. Taken together, our findings suggest that grafting the naphthalene dioxygenase complex into plants is a possible strategy to breed PAH-tolerant plants to efficiently degrade PAHs in the environment.
authors.affiliation: Shanghai Key Laboratory of Agricultural Genetics and Breeding, Agro-Biotechnology Research institute, Shanghai Academy of Agricultural Sciences, National Center for Plant Gene Research, Shanghai, PR China.
authors.forename: Rihe
authors.initials: R
authors.lastname: Peng
authors.affiliation: Shanghai Key Laboratory of Agricultural Genetics and Breeding, Agro-Biotechnology Research institute, Shanghai Academy of Agricultural Sciences, National Center for Plant Gene Research, Shanghai, PR China.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Fu
authors.affiliation: Shanghai Key Laboratory of Agricultural Genetics and Breeding, Agro-Biotechnology Research institute, Shanghai Academy of Agricultural Sciences, National Center for Plant Gene Research, Shanghai, PR China.
authors.forename: Yongsheng
authors.initials: Y
authors.lastname: Tian
authors.affiliation: Shanghai Key Laboratory of Agricultural Genetics and Breeding, Agro-Biotechnology Research institute, Shanghai Academy of Agricultural Sciences, National Center for Plant Gene Research, Shanghai, PR China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhao
authors.affiliation: Shanghai Key Laboratory of Agricultural Genetics and Breeding, Agro-Biotechnology Research institute, Shanghai Academy of Agricultural Sciences, National Center for Plant Gene Research, Shanghai, PR China.
authors.forename: Bo
authors.initials: B
authors.lastname: Zhu
authors.affiliation: Shanghai Key Laboratory of Agricultural Genetics and Breeding, Agro-Biotechnology Research institute, Shanghai Academy of Agricultural Sciences, National Center for Plant Gene Research, Shanghai, PR China.
authors.forename: Jing
authors.initials: J
authors.lastname: Xu
authors.affiliation: Shanghai Key Laboratory of Agricultural Genetics and Breeding, Agro-Biotechnology Research institute, Shanghai Academy of Agricultural Sciences, National Center for Plant Gene Research, Shanghai, PR China.
authors.forename: Bo
authors.initials: B
authors.lastname: Wang
authors.affiliation: Shanghai Key Laboratory of Agricultural Genetics and Breeding, Agro-Biotechnology Research institute, Shanghai Academy of Agricultural Sciences, National Center for Plant Gene Research, Shanghai, PR China.
authors.forename: Lijuan
authors.initials: L
authors.lastname: Wang
authors.affiliation: Shanghai Key Laboratory of Agricultural Genetics and Breeding, Agro-Biotechnology Research institute, Shanghai Academy of Agricultural Sciences, National Center for Plant Gene Research, Shanghai, PR China. Electronic address: yaoquanhong88@yahoo.com.
authors.forename: Quanhong
authors.initials: Q
authors.lastname: Yao
mesh_terms: D017360:Arabidopsis / Q000502:physiology*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D001673:Biodegradation, Environmental; D049308:Dioxygenases / Q000235:genetics / Q000378:metabolism*; D004785:Environmental Pollutants / Q000378:metabolism*; D024861:Genetic Enhancement / Q000379:methods; D060847:Metabolic Engineering / Q000379:methods*; D009097:Multienzyme Complexes / Q000235:genetics / Q000378:metabolism; D009281:Naphthalenes / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism; D011084:Polycyclic Aromatic Hydrocarbons / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D004785:Environmental Pollutants; D009097:Multienzyme Complexes; D009281:Naphthalenes; D011084:Polycyclic Aromatic Hydrocarbons; C031721:naphthalene; D049308:Dioxygenases
keywords: Hybrid naphthalene dioxygenase complex; Metabolic engineering; Phytoremediation; Polycyclic aromatic hydrocarbons; Transgenic Arabidopsis