title: Labware additives identified to be selective monoamine oxidase-B inhibitors.
abstract: Plastic labware is used in all processes of modern pharmaceutical research, including compound storage and biological assays. The use of these plastics has created vast increases in productivity and cost savings as experiments moved from glass test tubes and capillary pipettes to plastic microplates and multichannel liquid handlers. One consequence of the use of plastic labware, however, is the potential release of contaminants and their resultant effects on biological assays. We report herein the identification of biologically active substances released from a commonly used plastic microplate. The active contaminants were identified by gas chromatography-mass spectroscopy as dodecan-1-ol, dodecyl 3-(3-dodecoxy-3-oxopropyl)sulfanylpropanoate, and dodecanoic acid, and they were found to be selective monoamine oxidase-B inhibitors.
authors.affiliation: Leads Discovery & Optimization, Research and Development, Bristol-Myers Squibb Co., Wallingford, CT, USA jeremy.stewart@bms.com.
authors.forename: Jeremy
authors.initials: J
authors.lastname: Stewart
authors.affiliation: Pharmaceutical Candidate Optimization, Research and Development, Bristol-Myers Squibb Co., Wallingford, CT, USA.
authors.forename: Dieter M
authors.initials: DM
authors.lastname: Drexler
authors.affiliation: Synthesis & Analytical Technology Team, Research and Development, Bristol-Myers Squibb Co., Wallingford, CT, USA.
authors.forename: John E
authors.initials: JE
authors.lastname: Leet
authors.affiliation: Pharmaceutical Candidate Optimization, Research and Development, Bristol-Myers Squibb Co., Wallingford, CT, USA.
authors.forename: Colleen A
authors.initials: CA
authors.lastname: McNaney
authors.affiliation: Leads Discovery & Optimization, Research and Development, Bristol-Myers Squibb Co., Wallingford, CT, USA.
authors.forename: John J
authors.initials: JJ
authors.lastname: Herbst
mesh_terms: D015097:3-Mercaptopropionic Acid / Q000031:analogs & derivatives / Q000494:pharmacology; D007851:Dodecanol / Q000737:chemistry / Q000494:pharmacology; D004353:Drug Evaluation, Preclinical / Q000295:instrumentation* / Q000379:methods; D008401:Gas Chromatography-Mass Spectrometry; D007850:Lauric Acids / Q000494:pharmacology; D008995:Monoamine Oxidase / Q000378:metabolism; D008996:Monoamine Oxidase Inhibitors / Q000494:pharmacology*; D010969:Plastics / Q000737:chemistry* / Q000494:pharmacology; D059629:Signal-To-Noise Ratio; D013440:Sulfides / Q000494:pharmacology
chemical_list: D007850:Lauric Acids; D008996:Monoamine Oxidase Inhibitors; D010969:Plastics; D013440:Sulfides; C000602294:dodecyl 3-(3-dodecoxy-3-oxopropyl)sulfanylpropanoate; D007851:Dodecanol; D015097:3-Mercaptopropionic Acid; D008995:Monoamine Oxidase
keywords: dilauryl thiodipropionate (DLTDP); disposable plastic labware; extractable; leachable; monoamine oxidase