title: Chemical constituents with NO production inhibitory and cytotoxic activities from Litsea cubeba.
abstract: A bioassay-guided fractionation of the roots and stems of Litsea cubeba led to the isolation of 14 constituents, including three new compounds, cubebanone (1), N-cis-3,4-methylenedioxycinnamoyl-3-methoxytyramine (2), and 9,9'-O-di-(E)-feruloyl-(+)-secoisolariciresinol (3), together with 11 known ones (4-14). Compounds 1, 4, and 8-11 showed obvious in vitro anti-inflammatory activities against NO production in LPS-induced murine microglial (BV-2) cell line and RAW 264.7 macrophages. A cytotoxic evaluation showed that 2, 3, and 5-8 exhibited considerable inhibition against the growth of hepatocyte carcinoma (HepG2) cell line. These findings are evidence, to some extent, for the traditional medicinal application of L. cubeba, especially as folk medicine in the treatment of inflammation-related diseases.
authors.affiliation: Modern Research Center for Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing, 100029, People's Republic of China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Guo
authors.forename: Kewu
authors.initials: K
authors.lastname: Zeng
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Gao
authors.forename: Zhixiang
authors.initials: Z
authors.lastname: Zhu
authors.forename: Shuiying
authors.initials: S
authors.lastname: Zhang
authors.forename: Xingyun
authors.initials: X
authors.lastname: Chai
authors.forename: Pengfei
authors.initials: P
authors.lastname: Tu
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology / Q000633:toxicity; D002460:Cell Line; D045744:Cell Line, Tumor; D003603:Cytotoxins / Q000737:chemistry / Q000302:isolation & purification / Q000633:toxicity; D032862:Litsea / Q000737:chemistry*; D051379:Mice; D009569:Nitric Oxide / Q000096:biosynthesis*; D018517:Plant Roots / Q000737:chemistry; D018547:Plant Stems / Q000737:chemistry
chemical_list: D000893:Anti-Inflammatory Agents; D003603:Cytotoxins; D009569:Nitric Oxide