title: Age effects on tinnitus and hearing loss in CBA/CaJ mice following sound exposure.
abstract: Tinnitus is a maladaptive neuropathic condition that develops in humans and laboratory animals following auditory insult. In our previous study we demonstrated that sound exposure leads to development of behavioral evidence of tinnitus in a sample of exposed mice. However, this tinnitus mouse model did not account for long-term maladaptive plasticity or aging, factors that are commonly linked to the human tinnitus population. Therefore the same group of mice was monitored for tinnitus for 360 days post exposure. Tinnitus was assessed behaviorally by measuring gap-induced pre-pulse suppression of the acoustic startle (GPIAS). Cochlear histology was performed on both control (unexposed) and experimental mice to determine whether sound exposure caused any evident cochlear damage. We found that 360 days after exposure the vast majority of exposed mice exhibited similar gap detection deficits as detected at 84 days post exposure. These mice did not demonstrate significant loss of inner/outer hair cells or spiral ganglion neurons compared to the control sample. Lastly, we demonstrated that GPIAS deficits observed in exposed animals were unlikely exclusively caused by cochlear damage, but could be a result of central auditory maladaptive plasticity. We conclude that CBA/CaJ mice can be considered a good animal model to study the possible contribution of age effects on tinnitus development following auditory insult.
authors.affiliation: Department of Anatomy and Neurobiology, Northeast Ohio Medical University, 4209 State Route 44, Rootstown, OH 44272 USA ; Kent State University, Kent, OH 44240 USA.
authors.forename: Ryan J
authors.initials: RJ
authors.lastname: Longenecker
authors.affiliation: Department of Biology, Case Western Reserve University, Cleveland, OH 44106 USA.
authors.forename: Kurt T
authors.initials: KT
authors.lastname: Chonko
authors.affiliation: Department of Pediatrics, Richard King Mellon Foundation Institute for Pediatric Research, University of Pittsburgh, Pittsburgh, PA 15224 USA.
authors.forename: Steve M
authors.initials: SM
authors.lastname: Maricich
authors.affiliation: Department of Anatomy and Neurobiology, Northeast Ohio Medical University, 4209 State Route 44, Rootstown, OH 44272 USA.
authors.forename: Alexander V
authors.initials: AV
authors.lastname: Galazyuk
keywords: Acoustic trauma; Age-related hearing loss; Gap-induced suppression of the acoustic startle reflex; Tinnitus development