title: Molecular and Biological Characterization of an Isolate of Cucumber mosaic virus from Glycine soja by Generating its Infectious Full-genome cDNA Clones.
abstract: Molecular and biological characteristics of an isolate of Cucumber mosaic virus (CMV) from Glycine soja (wild soybean), named as CMV-209, was examined in this study. Comparison of nucleotide sequences and phylogenetic analyses of CMV-209 with the other CMV strains revealed that CMV-209 belonged to CMV subgroup I. However, CMV-209 showed some genetic distance from the CMV strains assigned to subgroup IA or subgroup IB. Infectious full-genome cDNA clones of CMV-209 were generated under the control of the Cauliflower mosaic virus 35S promoter. Infectivity of the CMV-209 clones was evaluated in Nicotiana benthamiana and various legume species. Our assays revealed that CMV-209 could systemically infect Glycine soja (wild soybean) and Pisum sativum (pea) as well as N. benthamiana, but not the other legume species.
authors.affiliation: Department of Agricultural Biotechnology and Plant Genomics and Breeding Institute, Seoul National University, Seoul 151-921, Korea.
authors.forename: Mi Sa Vo
authors.initials: MS
authors.lastname: Phan
authors.affiliation: Crop Protection Division, National Academy of Agricultural Science, Rural Development Administration, Suwon 441-707, Korea.
authors.forename: Jang-Kyun
authors.initials: JK
authors.lastname: Seo
authors.affiliation: Crop Protection Division, National Academy of Agricultural Science, Rural Development Administration, Suwon 441-707, Korea.
authors.forename: Hong-Soo
authors.initials: HS
authors.lastname: Choi
authors.affiliation: Department of Applied Biology, Kyungpook National University, Daegu 702-701, Korea.
authors.forename: Su-Heon
authors.initials: SH
authors.lastname: Lee
authors.affiliation: Department of Agricultural Biotechnology and Plant Genomics and Breeding Institute, Seoul National University, Seoul 151-921, Korea ; Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul 151-921, Korea.
authors.forename: Kook-Hyung
authors.initials: KH
authors.lastname: Kim
keywords: Cucumber mosaic virus; infectious clones; phylogenetic analyses; sequence analysis; wild soybean