title: Non-invasive prenatal chromosomal aneuploidy testing--clinical experience: 100,000 clinical samples.
abstract: OBJECTIVE
  As the first laboratory to offer massively parallel sequencing-based noninvasive prenatal testing (NIPT) for fetal aneuploidies, Sequenom Laboratories has been able to collect the largest clinical population experience data to date, including >100,000 clinical samples from all 50 U.S. states and 13 other countries. The objective of this study is to give a robust clinical picture of the current laboratory performance of the MaterniT21 PLUS LDT.
  
  
  METHODS
  The study includes plasma samples collected from patients with high-risk pregnancies in our CLIA-licensed, CAP-accredited laboratory between August 2012 to June 2013. Samples were assessed for trisomies 13, 18, 21 and for the presence of chromosome Y-specific DNA. Sample data and ad hoc outcome information provided by the clinician was compiled and reviewed to determine the characteristics of this patient population, as well as estimate the assay performance in a clinical setting.
  
  
  RESULTS
  NIPT patients most commonly undergo testing at an average of 15 weeks, 3 days gestation; and average 35.1 years of age. The average turnaround time is 4.54 business days and an overall 1.3% not reportable rate. The positivity rate for Trisomy 21 was 1.51%, followed by 0.45% and 0.21% rate for Trisomies 18 and 13, respectively. NIPT positivity rates are similar to previous large clinical studies of aneuploidy in women of maternal age ≥ 35 undergoing amniocentesis. In this population 3519 patients had multifetal gestations (3.5%) with 2.61% yielding a positive NIPT result.
  
  
  CONCLUSIONS
  NIPT has been commercially offered for just over 2 years and the clinical use by patients and clinicians has increased significantly. The risks associated with invasive testing have been substantially reduced by providing another assessment of aneuploidy status in high-risk patients. The accuracy and NIPT assay positivity rate are as predicted by clinical validations and the test demonstrates improvement in the current standard of care.
authors.affiliation: Clinical Science, Sequenom Laboratories, San Diego, California, United States of America.
authors.forename: Ron M
authors.initials: RM
authors.lastname: McCullough
authors.affiliation: Clinical Science, Sequenom Laboratories, San Diego, California, United States of America.
authors.forename: Eyad A
authors.initials: EA
authors.lastname: Almasri
authors.affiliation: Clinical Science, Sequenom Laboratories, San Diego, California, United States of America.
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Guan
authors.affiliation: Clinical Science, Sequenom Laboratories, San Diego, California, United States of America.
authors.forename: Jennifer A
authors.initials: JA
authors.lastname: Geis
authors.affiliation: Clinical Science, Sequenom Laboratories, San Diego, California, United States of America.
authors.forename: Susan C
authors.initials: SC
authors.lastname: Hicks
authors.affiliation: Clinical Science, Sequenom Laboratories, San Diego, California, United States of America.
authors.forename: Amin R
authors.initials: AR
authors.lastname: Mazloom
authors.affiliation: Clinical Science, Sequenom Laboratories, San Diego, California, United States of America.
authors.forename: Cosmin
authors.initials: C
authors.lastname: Deciu
authors.affiliation: Clinical Science, Sequenom Laboratories, San Diego, California, United States of America.
authors.forename: Paul
authors.initials: P
authors.lastname: Oeth
authors.affiliation: Clinical Science, Sequenom Laboratories, San Diego, California, United States of America.
authors.forename: Allan T
authors.initials: AT
authors.lastname: Bombard
authors.affiliation: Clinical Science, Sequenom Laboratories, San Diego, California, United States of America.
authors.forename: Bill
authors.initials: B
authors.lastname: Paxton
authors.affiliation: Clinical Science, Sequenom Laboratories, San Diego, California, United States of America.
authors.forename: Nilesh
authors.initials: N
authors.lastname: Dharajiya
authors.affiliation: Clinical Science, Sequenom Laboratories, San Diego, California, United States of America.
authors.forename: Juan-Sebastian
authors.initials: JS
authors.lastname: Saldivar
mesh_terms: D000328:Adult; D000782:Aneuploidy; D025063:Chromosome Disorders / Q000175:diagnosis; D005260:Female; D005820:Genetic Testing / Q000379:methods / Q000592:standards; D006801:Humans; D011247:Pregnancy; D011296:Prenatal Diagnosis / Q000379:methods / Q000592:standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity