title: Differential expression of collectins in human placenta and role in inflammation during spontaneous labor.
abstract: Collectins, collagen-containing Ca(2+) dependent C-type lectins and a class of secretory proteins including SP-A, SP-D and MBL, are integral to immunomodulation and innate immune defense. In the present study, we aimed to investigate their placental transcript synthesis, labor associated differential expression and localization at feto-maternal interface, and their functional implication in spontaneous labor. The study involved using feto-maternal interface (placental/decidual tissues) from two groups of healthy pregnant women at term (≥ 37 weeks of gestation), undergoing either elective C-section with no labor ('NLc' group, n = 5), or normal vaginal delivery with spontaneous labor ('SLv' group, n = 5). The immune function of SP-D, on term placental explants, was analyzed for cytokine profile using multiplexed cytokine array. SP-A, SP-D and MBL transcripts were observed in the term placenta. The 'SLv' group showed significant up-regulation of SP-D (p = 0.001), and down-regulation of SP-A (p = 0.005), transcripts and protein compared to the 'NLc' group. Significant increase in 43 kDa and 50 kDa SP-D forms in placental and decidual tissues was associated with the spontaneous labor (p<0.05). In addition, the MMP-9-cleaved form of SP-D (25 kDa) was significantly higher in the placentae of 'SLv' group compared to the 'NLc' group (p = 0.002). Labor associated cytokines IL-1α, IL-1β, IL-6, IL-8, IL-10, TNF-α and MCP-1 showed significant increase (p<0.05) in a dose dependent manner in the placental explants treated with nSP-D and rhSP-D. In conclusion, the study emphasizes that SP-A and SP-D proteins associate with the spontaneous labor and SP-D plausibly contributes to the pro-inflammatory immune milieu of feto-maternal tissues.
authors.affiliation: Department of Innate Immunity, National Institute for Research in Reproductive Health (Indian Council of Medical Research), Mumbai, Maharashtra, India.
authors.forename: Ajit Kumar
authors.initials: AK
authors.lastname: Yadav
authors.affiliation: Department of Obstetrics and Gynecology, Seth Gordhandas Sunderdas Medical College and King Edward Medical (KEM) Hospital, Mumbai, Maharashtra, India.
authors.forename: Hemangi
authors.initials: H
authors.lastname: Chaudhari
authors.affiliation: Department of Obstetrics and Gynecology, Seth Gordhandas Sunderdas Medical College and King Edward Medical (KEM) Hospital, Mumbai, Maharashtra, India.
authors.forename: Himangi
authors.initials: H
authors.lastname: Warke
authors.affiliation: Department of Obstetrics and Gynecology, Seth Gordhandas Sunderdas Medical College and King Edward Medical (KEM) Hospital, Mumbai, Maharashtra, India.
authors.forename: Premanand Keshavlal
authors.initials: PK
authors.lastname: Shah
authors.affiliation: Centre for Infection, Immunity and Disease Mechanisms, College of Health and Life Sciences, Brunel University London, Uxbridge, United Kingdom.
authors.forename: Eswari
authors.initials: E
authors.lastname: Dodagatta-Marri
authors.affiliation: Centre for Infection, Immunity and Disease Mechanisms, College of Health and Life Sciences, Brunel University London, Uxbridge, United Kingdom.
authors.forename: Uday
authors.initials: U
authors.lastname: Kishore
authors.affiliation: Department of Innate Immunity, National Institute for Research in Reproductive Health (Indian Council of Medical Research), Mumbai, Maharashtra, India.
authors.forename: Taruna
authors.initials: T
authors.lastname: Madan
mesh_terms: D000328:Adult; D002585:Cesarean Section; D037222:Collectins / Q000032:analysis* / Q000276:immunology; D016207:Cytokines / Q000032:analysis / Q000276:immunology; D005260:Female; D006801:Humans; D007249:Inflammation / Q000276:immunology; D007743:Labor, Obstetric; D009321:Natural Childbirth; D010920:Placenta / Q000737:chemistry* / Q000276:immunology; D011247:Pregnancy; D055815:Young Adult
chemical_list: D037222:Collectins; D016207:Cytokines