title: Peripheral blood mitochondrial DNA copy number is associated with prostate cancer risk and tumor burden.
abstract: Alterations of mitochondrial DNA (mtDNA) have been associated with the risk of a number of human cancers; however, the relationship between mtDNA copy number in peripheral blood leukocytes (PBLs) and the risk of prostate cancer (PCa) has not been investigated. In a case-control study of 196 PCa patients and 196 age-paired healthy controls in a Chinese Han population, the association between mtDNA copy number in PBLs and PCa risk was evaluated. The relative mtDNA copy number was measured using quantitative real-time PCR; samples from three cases and two controls could not be assayed, leaving 193 cases and 194 controls for analysis. PCa patients had significantly higher mtDNA copy numbers than controls (medians 0.91 and 0.82, respectively; P<0.001). Dichotomized at the median value of mtDNA copy number in the controls, high mtDNA copy number was significantly associated with an increased risk of PCa (adjusted odds ratio= 1.85, 95% confidence interval: 1.21-2.83). A significant dose-response relationship was observed between mtDNA copy number and risk of PCa in quartile analysis (Ptrend = 0.011). Clinicopathological analysis showed that high mtDNA copy numbers in PCa patients were significantly associated with high Gleason score and advanced tumor stage, but not serum prostate-specific antigen level (P = 0.002, 0.012 and 0.544, respectively). These findings of the present study indicate that increased mtDNA copy number in PBLs is significantly associated with an increased risk of PCa and may be a reflection of tumor burden.
authors.affiliation: Department of Urology, The Third Xiangya Hospital, Central South University, Changsha, China; Department of Abdominal Surgery, Jiangxi Cancer Hospital, Nanchang, China.
authors.forename: Weimin
authors.initials: W
authors.lastname: Zhou
authors.affiliation: Molecular Biology Research Center, Xiangya School of Medicine, Central South University, Changsha, China.
authors.forename: Min
authors.initials: M
authors.lastname: Zhu
authors.affiliation: Department of Nephrology, The Third Xiangya Hospital, Central South University, Changsha, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Gui
authors.affiliation: Center for Medical Experiments, The Third Xiangya Hospital, Central South University, Changsha, China.
authors.forename: Lihua
authors.initials: L
authors.lastname: Huang
authors.affiliation: Department of Urology, The Third Xiangya Hospital, Central South University, Changsha, China.
authors.forename: Zhi
authors.initials: Z
authors.lastname: Long
authors.affiliation: Department of Nephrology, The Third Xiangya Hospital, Central South University, Changsha, China.
authors.forename: Li
authors.initials: L
authors.lastname: Wang
authors.affiliation: Department of Epidemiology and Statistics, Public Health School, Central South University, Changsha, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Chen
authors.affiliation: Department of Urology, The Third Xiangya Hospital, Central South University, Changsha, China.
authors.forename: Yinghao
authors.initials: Y
authors.lastname: Yin
authors.affiliation: Department of Urology, The Third Xiangya Hospital, Central South University, Changsha, China.
authors.forename: Xianzhen
authors.initials: X
authors.lastname: Jiang
authors.affiliation: Department of Urology, The Third Xiangya Hospital, Central South University, Changsha, China.
authors.forename: Yingbo
authors.initials: Y
authors.lastname: Dai
authors.affiliation: Department of Urology, The Third Xiangya Hospital, Central South University, Changsha, China.
authors.forename: Yuxin
authors.initials: Y
authors.lastname: Tang
authors.affiliation: Department of Urology, The Third Xiangya Hospital, Central South University, Changsha, China.
authors.forename: Leye
authors.initials: L
authors.lastname: He
authors.affiliation: Department of Urology, The Third Xiangya Hospital, Central South University, Changsha, China.
authors.forename: Kuangbiao
authors.initials: K
authors.lastname: Zhong
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics / Q000473:pathology; D000368:Aged; D016022:Case-Control Studies; D056915:DNA Copy Number Variations / Q000235:genetics*; D004272:DNA, Mitochondrial / Q000235:genetics*; D005500:Follow-Up Studies; D006801:Humans; D007962:Leukocytes / Q000378:metabolism / Q000473:pathology*; D008297:Male; D008928:Mitochondria / Q000378:metabolism / Q000473:pathology*; D060787:Neoplasm Grading; D009367:Neoplasm Staging; D011379:Prognosis; D011471:Prostatic Neoplasms / Q000235:genetics* / Q000473:pathology*; D060888:Real-Time Polymerase Chain Reaction; D012189:Retrospective Studies; D012307:Risk Factors; D047368:Tumor Burden
chemical_list: D004272:DNA, Mitochondrial