title: Wafer-scale fabrication of glass-FEP-glass microfluidic devices for lipid bilayer experiments.
abstract: We report a wafer-scale fabrication process for the production of glass-FEP-glass microdevices using UV-curable adhesive (NOA81) as gluing material, which is applied using a novel "spin & roll" approach. Devices are characterized for the uniformity of the gluing layer, presence of glue in the microchannels, and alignment precision. Experiments on lipid bilayers with electrophysiological recordings using a model pore-forming polypeptide are demonstrated.
authors.affiliation: BIOS - Lab on a Chip group, MESA+ Institute for Nanotechnology, MIRA Institute for Biomedical Technology and Technical Medicine, University of Twente, Enschede, The Netherlands. s.legac@utwente.nl.
authors.forename: Johan G
authors.initials: JG
authors.lastname: Bomer
authors.forename: Alexander V
authors.initials: AV
authors.lastname: Prokofyev
authors.forename: Albert
authors.initials: A
authors.lastname: van den Berg
authors.forename: Séverine
authors.initials: S
authors.lastname: Le Gac
mesh_terms: D000269:Adhesives; D004594:Electrophysiology; D004867:Equipment Design; D005898:Glass / Q000737:chemistry*; D008051:Lipid Bilayers / Q000737:chemistry*; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D011138:Polytetrafluoroethylene / Q000031:analogs & derivatives* / Q000737:chemistry; D005465:Fluorocarbon Polymers
chemical_list: D000269:Adhesives; D008051:Lipid Bilayers; D011138:Polytetrafluoroethylene; C096305:fluorinated ethylene propylene; D005465:Fluorocarbon Polymers