title: Changes in cerebrospinal fluid biomarkers in human herpesvirus-6-associated acute encephalopathy/febrile seizures.
abstract: To determine the involvement of oxidative stress in the pathogenesis of acute encephalopathy associated with human herpesvirus-6 (HHV-6) infection, we measured the levels of oxidative stress markers 8-hydroxy-2'-deoxyguanosine (8-OHdG) and hexanoyl-lysine adduct (HEL), tau protein, and cytokines in cerebrospinal fluid (CSF) obtained from patients with HHV-6-associated acute encephalopathy (HHV-6 encephalopathy) (n = 16) and complex febrile seizures associated with HHV-6 (HHV-6 complex FS) (n = 10). We also examined changes in CSF-8OHdG and CSF-HEL levels in patients with HHV-6 encephalopathy before and after treatment with edaravone, a free radical scavenger. CSF-8-OHdG levels in HHV-6 encephalopathy and HHV-6 complex FS were significantly higher than in control subjects. In contrast, CSF-HEL levels showed no significant difference between groups. The levels of total tau protein in HHV-6 encephalopathy were significantly higher than in control subjects. In six patients with HHV-6 infection (5 encephalopathy and 1 febrile seizure), the CSF-8-OHdG levels of five patients decreased after edaravone treatment. Our results suggest that oxidative DNA damage is involved in acute encephalopathy associated with HHV-6 infection.
authors.affiliation: Department of Pediatrics, Tokyo Metropolitan Fuchu Medical Center for the Disabled, 2-9-2 Musashidai, Fuchu, Tokyo 183-8553, Japan ; Department of Brain Development and Neural Regeneration, Tokyo Metropolitan Institute of Medical Science, Tokyo 156-8506, Japan.
authors.forename: Naoyuki
authors.initials: N
authors.lastname: Tanuma
authors.affiliation: Department of Brain Development and Neural Regeneration, Tokyo Metropolitan Institute of Medical Science, Tokyo 156-8506, Japan.
authors.forename: Rie
authors.initials: R
authors.lastname: Miyata
authors.affiliation: Department of Brain Development and Neural Regeneration, Tokyo Metropolitan Institute of Medical Science, Tokyo 156-8506, Japan.
authors.forename: Keisuke
authors.initials: K
authors.lastname: Nakajima
authors.affiliation: Department of Pediatrics, School of Medicine, Juntendo University, Tokyo 113-8421, Japan.
authors.forename: Akihisa
authors.initials: A
authors.lastname: Okumura
authors.affiliation: Department of Neurology, National Center for Child Health and Development, Tokyo 157-8535, Japan.
authors.forename: Masaya
authors.initials: M
authors.lastname: Kubota
authors.affiliation: Division of Neurology, Saitama Children's Medical Center, Saitama 339-8551, Japan.
authors.forename: Shin-ichiro
authors.initials: S
authors.lastname: Hamano
authors.affiliation: Department of Brain Development and Neural Regeneration, Tokyo Metropolitan Institute of Medical Science, Tokyo 156-8506, Japan.
authors.forename: Masaharu
authors.initials: M
authors.lastname: Hayashi
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D000293:Adolescent; D000983:Antipyrine / Q000031:analogs & derivatives* / Q000627:therapeutic use; D015415:Biomarkers / Q000134:cerebrospinal fluid*; D002648:Child; D004249:DNA Damage / Q000502:physiology; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000134:cerebrospinal fluid; D000077553:Edaravone; D005260:Female; D015654:Herpesvirus 6, Human / Q000472:pathogenicity*; D006801:Humans; D008297:Male; D019349:Roseolovirus Infections / Q000134:cerebrospinal fluid* / Q000188:drug therapy / Q000378:metabolism; D003294:Seizures, Febrile / Q000188:drug therapy / Q000378:metabolism* / Q000821:virology*; D055815:Young Adult
chemical_list: D015415:Biomarkers; D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine; D000077553:Edaravone; D000983:Antipyrine