title: Efficient biotransformation of polysialogangliosides for preparation of GM1 by Cellulosimicrobium sp. 21.
abstract: A new ganglioside transformed strain isolated from soil was identified as Cellulosimicrobium sp. 21. It produced a sialidase which transformed polysialo-gangliosides GD1 and GT1 into a monosialoterahexosylganglioside, i.e., ganglioside GM1. The sialidase had both NeuAc-α-2,3- and NeuAc-α-2,8-sialidase activity without producing asiolo-GM1. The optimum conditions were evaluated and it was found that the transformation was optimally performed at 30 °C and pH 7.0. The substrate should be added at the beginning of the reaction and the concentration of substrate was 3% (w/v). Under these optimum conditions, Cellulosimicrobium sp. 21 converted GD1 and GT1 into GM1 in inorganic medium in a 5 L bioreactor with the recovery rate of 69.3%. The product contained 50.3% GM1 and was purified on silica to give the product with 95% of GM1 with a recovery rate of 30.5%. Therefore, Cellulosimicrobium sp. 21 has potential to be applied in the production of GM1 in the pharmaceutical industry.
authors.affiliation: China-Japan Union Hospital, Jilin University, Changchun 130033, Jilin, China. zhy19780603@aliyun.com.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zheng
authors.affiliation: School of Life Sciences, Northeast Normal University, Changchun 130024, Jilin, China. jil132@nenu.edu.cn.
authors.forename: Li
authors.initials: L
authors.lastname: Ji
authors.affiliation: School of Life Sciences, Northeast Normal University, Changchun 130024, Jilin, China. lengjy366@nenu.edu.cn.
authors.forename: Jiayi
authors.initials: J
authors.lastname: Leng
authors.affiliation: School of Life Sciences, Northeast Normal University, Changchun 130024, Jilin, China. yuany268@nenu.edu.cn.
authors.forename: Ye
authors.initials: Y
authors.lastname: Yuan
authors.affiliation: School of Life Sciences, Northeast Normal University, Changchun 130024, Jilin, China. chenhl150@nenu.edu.cn.
authors.forename: Honglei
authors.initials: H
authors.lastname: Chen
authors.affiliation: School of Life Sciences, Northeast Normal University, Changchun 130024, Jilin, China. goudx479@nenu.edu.cn.
authors.forename: Dongxia
authors.initials: D
authors.lastname: Gou
authors.affiliation: China-Japan Union Hospital, Jilin University, Changchun 130033, Jilin, China. gaoyufei1975@sina.cn.
authors.forename: Yufei
authors.initials: Y
authors.lastname: Gao
authors.affiliation: School of Life Sciences, Northeast Normal University, Changchun 130024, Jilin, China. zhouyf383@nenu.edu.cn.
authors.forename: Yifa
authors.initials: Y
authors.lastname: Zhou
mesh_terms: D039903:Actinobacteria / Q000378:metabolism*; D019149:Bioreactors; D005285:Fermentation; D005677:G(M1) Ganglioside / Q000096:biosynthesis* / Q000737:chemistry / Q000302:isolation & purification; D005732:Gangliosides / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D013379:Substrate Specificity; D013696:Temperature
chemical_list: D005732:Gangliosides; C021298:ganglioside, GD1a; D005677:G(M1) Ganglioside; C016481:trisialoganglioside GT1