title: Amentoflavone acts as a radioprotector for irradiated v79 cells by regulating reactive oxygen species (ROS), cell cycle and mitochondrial mass.
abstract: Radioprotective effects of amentoflavone were investigated by examining cell viability, apoptosis, cell cycling concentrations of intracellular ROS (reactive oxygen species), and relative mitochondrial mass by flow cytometry after 60Co irradiation. Pretreatment with amentoflavone 24 hours prior to 8 Gy 60Co γ-ray irradiation significantly inhibited apoptosis, promoted the G2 phase, decreased the concentration of ROS and mitochondrial mass. These results collectively indicate that amentoflavone is an effective radioprotective agent.
authors.affiliation: Department of Pharmacy, Xinxiang Medical University, Xinxiang, Henan, China E-mail : 13273730271@163.com.
authors.forename: Ping
authors.initials: P
authors.lastname: Xu
authors.forename: En-Jin
authors.initials: EJ
authors.lastname: Jiang
authors.forename: Si-Yuan
authors.initials: SY
authors.lastname: Wen
authors.forename: Dan-Dan
authors.initials: DD
authors.lastname: Lu
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects / Q000528:radiation effects; D044946:Biflavonoids / Q000494:pharmacology*; D002453:Cell Cycle / Q000187:drug effects* / Q000528:radiation effects; D049109:Cell Proliferation / Q000187:drug effects / Q000528:radiation effects; D002478:Cells, Cultured; D003037:Cobalt Radioisotopes; D006224:Cricetinae; D065688:Cytochrome P-450 CYP2C9 Inhibitors / Q000494:pharmacology; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D005434:Flow Cytometry; D005720:Gamma Rays; D008168:Lung / Q000166:cytology / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects* / Q000528:radiation effects; D008928:Mitochondria / Q000187:drug effects* / Q000378:metabolism / Q000528:radiation effects; D011837:Radiation-Protective Agents / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism*
chemical_list: D044946:Biflavonoids; D003037:Cobalt Radioisotopes; D065688:Cytochrome P-450 CYP2C9 Inhibitors; D011837:Radiation-Protective Agents; D017382:Reactive Oxygen Species; C011164:amentoflavone