title: Electronic tongue generating continuous recognition patterns for protein analysis.
abstract: In current protocol, a combinatorial approach has been developed to simplify the design and production of sensing materials for the construction of electronic tongues (eT) for protein analysis. By mixing a small number of simple and easily accessible molecules with different physicochemical properties, used as building blocks (BBs), in varying and controlled proportions and allowing the mixtures to self-assemble on the gold surface of a prism, an array of combinatorial surfaces featuring appropriate properties for protein sensing was created. In this way, a great number of cross-reactive receptors can be rapidly and efficiently obtained. By combining such an array of combinatorial cross-reactive receptors (CoCRRs) with an optical detection system such as surface plasmon resonance imaging (SPRi), the obtained eT can monitor the binding events in real-time and generate continuous recognition patterns including 2D continuous evolution profile (CEP) and 3D continuous evolution landscape (CEL) for samples in liquid. Such an eT system is efficient for discrimination of common purified proteins.
authors.affiliation: Structure et Propriétés d'Architectures Moléculaires, Institut Nanosciences et Cryogénie, CEA-Grenoble; yanxia.hou-broutin@cea.fr.
authors.forename: Yanxia
authors.initials: Y
authors.lastname: Hou
authors.affiliation: Structure et Propriétés d'Architectures Moléculaires, Institut Nanosciences et Cryogénie, CEA-Grenoble.
authors.forename: Maria
authors.initials: M
authors.lastname: Genua
authors.affiliation: Structure et Propriétés d'Architectures Moléculaires, Institut Nanosciences et Cryogénie, CEA-Grenoble.
authors.forename: Laurie-Amandine
authors.initials: LA
authors.lastname: Garçon
authors.affiliation: Structure et Propriétés d'Architectures Moléculaires, Institut Nanosciences et Cryogénie, CEA-Grenoble.
authors.forename: Arnaud
authors.initials: A
authors.lastname: Buhot
authors.affiliation: Structure et Propriétés d'Architectures Moléculaires, Institut Nanosciences et Cryogénie, CEA-Grenoble.
authors.forename: Roberto
authors.initials: R
authors.lastname: Calemczuk
authors.affiliation: Institut de Chimie Moléculaire et des Matériaux d'Orsay, Université Paris-Sud.
authors.forename: David
authors.initials: D
authors.lastname: Bonnaffé
authors.affiliation: Institut de Biologie Structurale.
authors.forename: Hugues
authors.initials: H
authors.lastname: Lortat-Jacob
authors.affiliation: Structure et Propriétés d'Architectures Moléculaires, Institut Nanosciences et Cryogénie, CEA-Grenoble.
authors.forename: Thierry
authors.initials: T
authors.lastname: Livache
mesh_terms: D032701:Biomimetics / Q000295:instrumentation*; D004581:Electronics / Q000295:instrumentation* / Q000379:methods; D011506:Proteins / Q000032:analysis*; D014059:Tongue
chemical_list: D011506:Proteins