title: The effect of colchicine and low-dose methotrexate on intestinal ischemia/reperfusion injury in an experimental model.
abstract: OBJECTIVE
  Intestinal ischemia/reperfusion (I/R) injury is a serious clinical condition. Colchicine and low-dose methotrexate have anti-inflammatory features. An experimental model was conducted to investigate the effect of colchicine and methotrexate on intestinal I/R injury.
  
  
  METHODS
  Twenty-four rats were included. Only laparotomy was done in control group (CG, n=6). In experimental groups, superior mesenteric artery was occluded. After 1h ischemia, reperfusion (1h) was started by de-occlusion. 30min before reperfusion, saline in sham group (SG, n:6), colchicine (1mg/kg) in colchicine group (CNG, n:6), and methotrexate (0.1mg/kg) in methotrexate group (MTXG, n:6) were infused intraperitoneally. Small intestines were harvested for evaluation of intestinal mucosal injury (Chiu score) and oxidative stress markers (nitric oxide: NO, malondialdehyde: MDA, superoxide dismutase: SOD).
  
  
  RESULTS
  Biochemically, MDA levels were significantly low in CG compared to SG, CNG, and MTXG (p<0.05). NO levels were significantly low and SOD levels were significantly high in CG compared to MTXG (p<0.05). Histopathologically, Chiu score was significantly low in CG compared to SG, CNG, and MTXG (p<0.05), and significantly high in MTXG compared to SG and CNG (p<0.05).
  
  
  CONCLUSIONS
  The present experimental model caused I/R injury in rat intestines. Contrary to literature, it was found that methotrexate worsens and colchicine does not attenuate intestinal I/R injury.
authors.affiliation: Kırıkkale University, Medical Faculty, Department of Pediatric Surgery, Kırıkkale, Turkey. Electronic address: ozlemboy80@yahoo.com.
authors.forename: Özlem
authors.initials: Ö
authors.lastname: Boybeyi
authors.affiliation: Kırıkkale University, Medical Faculty, Department of Pediatric Surgery, Kırıkkale, Turkey.
authors.forename: Yasemin Dere
authors.initials: YD
authors.lastname: Gunal
authors.affiliation: Kırıkkale University, Medical Faculty, Department of Pathology Kırıkkale, Turkey.
authors.forename: Pinar
authors.initials: P
authors.lastname: Atasoy
authors.affiliation: Kırıkkale University, Medical Faculty, Department of Biochemistry, Kırıkkale, Turkey.
authors.forename: Ucler
authors.initials: U
authors.lastname: Kısa
authors.affiliation: Kırıkkale University, Medical Faculty, Department of Pediatric Surgery, Kırıkkale, Turkey.
authors.forename: Mustafa Kemal
authors.initials: MK
authors.lastname: Aslan
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage*; D003078:Colchicine / Q000008:administration & dosage*; D004195:Disease Models, Animal; D007077:Ileal Diseases / Q000188:drug therapy*; D007422:Intestines / Q000098:blood supply / Q000473:pathology; D008297:Male; D008727:Methotrexate / Q000008:administration & dosage*; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000188:drug therapy*
chemical_list: D000893:Anti-Inflammatory Agents; D003078:Colchicine; D008727:Methotrexate
keywords: Colchicine; Experimental; Intestinal ischemia; Ischemia/reperfusion injury; Methotrexate