title: Retinal biocompatibility of brilliant blue g with deuterated water for chromovitrectomy.
abstract: OBJECTIVE
  To investigate the retinal biocompatibility of Brilliant Blue G with deuterated water (BBG-D2O) as a vital dye for chromovitrectomy.
  
  
  METHODS
  In this animal study, 0.05 mL of 0.25 g/L Brilliant Blue G (BBG) associated with 0.13 mL/mL of deuterium oxide (D2O) was injected intravitreally in the right eye and the same amount of balanced salt solution (BSS) was injected similarly in the left eye of rabbits. Clinical examination and histology with light microscopy were performed after seven days. Retinal cell layers were evaluated for morphologic alterations. Electroretinographic (ERG) changes were also assessed at baseline and 7 days after the injections.
  
  
  RESULTS
  A total of 6 rabbits were included in the study. The gross histopathologic appearance of the retina, choroid, sclera and optic nerve was within normal limits without any sign of severe retinal necrosis or cystic degeneration. Light microscopy showed that BBG-D2O caused no substantial alterations in retinal layers as compared to control eyes. The injection of BBG-D2O did not induce considerable functional ERG alterations.
  
  
  CONCLUSIONS
  Intravitreal injection of BBG-D2O 0.25 g/L seems to induce no retinal toxicity as documented by lack of functional and histological changes.
authors.affiliation: Department of Ophthalmology, Vision Institute (IPEPO), Federal University of São Paulo, São Paulo, Brazil.
authors.forename: Emmerson
authors.initials: E
authors.lastname: Badaró
authors.affiliation: Department of Ophthalmology, Vision Institute (IPEPO), Federal University of São Paulo, São Paulo, Brazil.
authors.forename: Milton
authors.initials: M
authors.lastname: Moraes-Filho
authors.affiliation: Department of Ophthalmology, Vision Institute (IPEPO), Federal University of São Paulo, São Paulo, Brazil.
authors.forename: Mauricio
authors.initials: M
authors.lastname: Maia
authors.affiliation: Department of Ophthalmology, Vision Institute (IPEPO), Federal University of São Paulo, São Paulo, Brazil.
authors.forename: Fernando M
authors.initials: FM
authors.lastname: Penha
authors.affiliation: Department of Ophthalmology, Vision Institute (IPEPO), Federal University of São Paulo, São Paulo, Brazil.
authors.forename: Eduardo Amorim
authors.initials: EA
authors.lastname: Novais
authors.affiliation: Department of Ophthalmology, Vision Institute (IPEPO), Federal University of São Paulo, São Paulo, Brazil.
authors.forename: Rodrigo A
authors.initials: RA
authors.lastname: Souza-Lima
authors.affiliation: Department of Ophthalmology, Vision Institute (IPEPO), Federal University of São Paulo, São Paulo, Brazil.
authors.forename: Flavio
authors.initials: F
authors.lastname: Hirai
authors.affiliation: Department of Ophthalmology, University of Bonn, Bonn, Germany.
authors.forename: Carsten H
authors.initials: CH
authors.lastname: Meyer
authors.affiliation: Department of Ophthalmology, Vision Institute (IPEPO), Federal University of São Paulo, São Paulo, Brazil.
authors.forename: Michel Eid
authors.initials: ME
authors.lastname: Farah
authors.affiliation: Department of Ophthalmology, Vision Institute (IPEPO), Federal University of São Paulo, São Paulo, Brazil.
authors.forename: Eduardo B
authors.initials: EB
authors.lastname: Rodrigues
keywords: Brillant Blue; Chromovitrectomy; Deuterated Water; Retinal Toxicity