title: Enhanced degradation of biphenyl from PCB-contaminated sediments: the impact of extracellular organic matter from Micrococcus luteus.
abstract: Recent advances in the bioremediation of polychlorinated biphenyl (PCB)-contaminated environments have focused on the development of approaches to stimulate the activities of indigenous bacterial communities. In this study, extracellular organic matter (EOM) from Micrococcus luteus was used to enhance the biphenyl-degrading capability of potentially functional microorganisms. The obtained results suggest that EOM significantly enhanced the biphenyl (BP)-degradation capability. Under a concentration of 3,500 mg/L BP, BP-degradation efficiency reached 60.8 % at a dosage of 10 % EOM (v/v), whereas the degradation efficiencies of control group (with inactivated EOM addition) and blank group (with lactate minimal medium) were only 21.5 and 6.2 %, respectively. Denaturing gradient gel electrophoresis (DGGE) profiles demonstrated that EOM played a key role in shifts in the composition and diversity of bacterial community. The Illumina high-throughput sequencing analysis indicated that the genera of Rhodococcus and Pseudomonas closely related to BP/PCB-degradation were greatly abundant after EOM addition. Together with polymerase chain reaction (PCR)-DGGE analysis, the link between the enhanced BP-degrading capability and the stimulation and resuscitation function of EOM in uncultured bacteria belonging to phylum Actinobacteria was tentatively established. These results suggest that EOM from M. luteus as an additive holds great potential for the efficient and cost-effective bioremediation of PCB-contaminated environment.
authors.affiliation: Department of Environmental Engineering, College of Environmental and Resource Sciences, Zhejiang University, Yuhangtang Road 866, Hangzhou, 310058, China.
authors.forename: Xiaomei
authors.initials: X
authors.lastname: Su
authors.forename: Qian
authors.initials: Q
authors.lastname: Zhang
authors.forename: Jinxing
authors.initials: J
authors.lastname: Hu
authors.forename: Muhammad Zaffar
authors.initials: MZ
authors.lastname: Hashmi
authors.forename: Linxian
authors.initials: L
authors.lastname: Ding
authors.forename: Chaofeng
authors.initials: C
authors.lastname: Shen
mesh_terms: D001419:Bacteria / Q000145:classification* / Q000235:genetics / Q000378:metabolism*; D058448:Biota; D001711:Biotransformation; D001713:Biphenyl Compounds / Q000378:metabolism*; D016000:Cluster Analysis; D003470:Culture Media / Q000737:chemistry; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D058645:Denaturing Gradient Gel Electrophoresis; D008969:Molecular Sequence Data; D010802:Phylogeny; D011078:Polychlorinated Biphenyls / Q000032:analysis*; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D014873:Water Pollutants / Q000378:metabolism*
chemical_list: D001713:Biphenyl Compounds; D003470:Culture Media; D004269:DNA, Bacterial; D011078:Polychlorinated Biphenyls; D014873:Water Pollutants; C010574:biphenyl