title: Synthesis, characterization and antioxidant activity of a novel electroactive and biodegradable polyurethane for cardiac tissue engineering application.
abstract: There has been a growing trend towards applying conducting polymers for electrically excitable cells to increase electrical signal propagation within the cell-loaded substrates. A novel biodegradable electroactive polyurethane containing aniline pentamer (AP-PU) was synthesized and fully characterized by spectroscopic methods. To tune the physico-chemical properties and biocompatibility, the AP-PU was blended with polycaprolactone (PCL). The presence of electroactive moieties and the electroactivity behavior of the prepared films were confirmed by UV-visible spectroscopy and cyclic voltammetry. A conventional four probe analysis demonstrated the electrical conductivity of the films in the semiconductor range (~10(-5)S/cm). MTT assays using L929 mouse fibroblast and human umbilical vein endothelial cells (HUVECs) showed that the prepared blend (PB) displayed more cytocompatibility compared with AP-PU due to the introduction of a biocompatible PCL moiety. The in vitro cell culture also confirmed that PB was as supportive as tissue culture plate. The antioxidant activity of the AP-PU was proved using 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging assay by employing UV-vis spectroscopy. In vitro degradation tests conducted in phosphate-buffered saline, pH7.4 and pH5.5, proved that the films were also biodegradable. The results of this study have highlighted the potential application of this bioelectroactive polyurethane as a platform substrate to study the effect of electrical signals on cell activities and to direct desirable cell function for tissue engineering applications.
authors.affiliation: Department of Tissue Engineering, School of Advanced Medical Technologies, Tehran University of Medical Sciences, 1417755469 Tehran, Iran.
authors.forename: Nafiseh
authors.initials: N
authors.lastname: Baheiraei
authors.affiliation: Department of Polyurethane, Iran Polymer and Petrochemical Institute, P.O. Box: 14965/115, Tehran, Iran. Electronic address: h.yeganeh@ippi.ac.ir.
authors.forename: Hamid
authors.initials: H
authors.lastname: Yeganeh
authors.affiliation: Department of Tissue Engineering, School of Advanced Medical Technologies, Tehran University of Medical Sciences, 1417755469 Tehran, Iran; Brain and Spinal Injury Research Center, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Jafar
authors.initials: J
authors.lastname: Ai
authors.affiliation: Department of Polyurethane, Iran Polymer and Petrochemical Institute, P.O. Box: 14965/115, Tehran, Iran.
authors.forename: Reza
authors.initials: R
authors.lastname: Gharibi
authors.affiliation: Department of Tissue Engineering, School of Advanced Medical Technologies, Tehran University of Medical Sciences, 1417755469 Tehran, Iran.
authors.forename: Mahmoud
authors.initials: M
authors.lastname: Azami
authors.affiliation: Department of Tissue Engineering, School of Advanced Medical Technologies, Tehran University of Medical Sciences, 1417755469 Tehran, Iran.
authors.forename: Faezeh
authors.initials: F
authors.lastname: Faghihi
mesh_terms: D000814:Aniline Compounds / Q000737:chemistry; D000818:Animals; D000975:Antioxidants / Q000737:chemistry; D001672:Biocompatible Materials / Q000737:chemistry; D001713:Biphenyl Compounds / Q000494:pharmacology; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D018914:Cells, Immobilized; D004553:Electric Conductivity; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D051379:Mice; D008855:Microscopy, Electron, Scanning; D032383:Myocytes, Cardiac / Q000187:drug effects / Q000378:metabolism; D010851:Picrates / Q000494:pharmacology; D011091:Polyesters / Q000737:chemistry; D011140:Polyurethanes / Q000737:chemistry*; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry
chemical_list: D000814:Aniline Compounds; D000975:Antioxidants; D001672:Biocompatible Materials; D001713:Biphenyl Compounds; D010851:Picrates; D011091:Polyesters; D011140:Polyurethanes; C016240:polycaprolactone; C004931:1,1-diphenyl-2-picrylhydrazyl; C023650:aniline
keywords: Aniline pentamer; Cardiac patch; Myocardial infarction; Polyurethane