title: Examining the effects of nuclear GPCRs on gene expression using isolated nuclei.
abstract: The measurement of changes in the transcriptome is a common end point for various pathologic and pharmacologic studies. In recent years, with the discovery of a host of potential pharmacologic targets located directly on the nuclear membrane, the need to assess their potential control over the transcriptome has arisen. Here we present techniques for assessing changes in gene expression in isolated nuclei in response to stimulation by endogenous GPCRs on the nuclear membrane.
authors.affiliation: Montreal Heart Institute, 5000 Belanger Street, Montreal, QC, Canada, H1T 1C8.
authors.forename: George
authors.initials: G
authors.lastname: Vaniotis
authors.forename: Sarah
authors.initials: S
authors.lastname: Gora
authors.forename: André
authors.initials: A
authors.lastname: Nantel
authors.forename: Terence E
authors.initials: TE
authors.lastname: Hébert
authors.forename: Bruce G
authors.initials: BG
authors.lastname: Allen
mesh_terms: D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism*; D015870:Gene Expression; D020869:Gene Expression Profiling / Q000379:methods; D009206:Myocardium / Q000378:metabolism; D051381:Rats; D018160:Receptors, Cytoplasmic and Nuclear / Q000378:metabolism*; D043562:Receptors, G-Protein-Coupled / Q000378:metabolism*; D015533:Transcriptional Activation; D059467:Transcriptome
chemical_list: D018160:Receptors, Cytoplasmic and Nuclear; D043562:Receptors, G-Protein-Coupled