title: [Method for simultaneous visualization of mast cells and nerve terminals in the rodent thymus].
abstract: The purpose of this study was to develop the method for the simultaneous visualization of mast cells (MCs) and nerve terminals, based on generally accepted techniques of histochemical identification of MCs with alcian blue and immunohistochemical detection of synaptophysin. The protocol presented allows simultaneous identification of mast cells and nerve terminals in the sections of paraffin-embedded thymus of laboratory mammals with high selectivity and good reproducibility. The method can be used for both visualization of spatial relationship between MCs and nerve terminals and independent research of the innervation of mammalian internal organs. Zinc-ethanol-formaldehyde is recommended as an optimal fixative.
authors.forename: V V
authors.initials: VV
authors.lastname: Gusel'nikova
authors.forename: E G
authors.initials: EG
authors.lastname: Sukhorukova
authors.forename: E A
authors.initials: EA
authors.lastname: Fedorova
authors.forename: A V
authors.initials: AV
authors.lastname: Polevshchikov
authors.forename: D É
authors.initials: DÉ
authors.lastname: Korzhevskiĭ
mesh_terms: D000818:Animals; D008407:Mast Cells / Q000166:cytology / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D010525:Peripheral Nerves / Q000166:cytology / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D016708:Synaptophysin / Q000378:metabolism*; D013950:Thymus Gland / Q000166:cytology / Q000294:innervation / Q000378:metabolism
chemical_list: D016708:Synaptophysin; C569016:Syp protein, rat