title: [Immunogenicity and safety of a prototype chemical anthrax vaccine in laboratory animal models].
abstract: OBJECTIVE
  Evaluation of immune stimulating and toxic effects of a vaccine prototype protein components.
  
  
  METHODS
  Linear mice, guinea pigs and rabbits were immunized subcutaneously once or twice by recombinant protective antigen (rPA), S-layer protein (EA1) or their complex. Innate immunity structure activation was registered by changes in Toll-like receptor (TLR) expression. Adaptive immune response parameters were determined by established methods. Toxicity of the preparations was determined using flow cytofluorometry and densitomorphometry.
  
  
  RESULTS
  The ability of rPA and EA1 to activate structures of innate immunity - TLR 2 and 6 - was established. Features of anti-PA antibody titer dynamics for each of the animal species was determined, a comparison with antibody formation during immunization with Bacillus anthracis STI- 1 was carried out. 2 immunizations ofbiomodels with a complex preparation combined with an adjuvant provides protection from infection by a test-strain that is comparable with protectivity of a live vaccine. Evidences regarding damaging effect of rPA and EAI on cells and tissues of macro organism were not detected throughout the study.
  
  
  CONCLUSIONS
  Aprototype of a chemical anthrax vaccine under development has high immunogenicity and its protein components are not toxic for laboratory animals based on the results of complex testing.
authors.forename: N I
authors.initials: NI
authors.lastname: Mikshis
authors.forename: P Iu
authors.initials: PIu
authors.lastname: Popova
authors.forename: O M
authors.initials: OM
authors.lastname: Kudriavtseva
authors.forename: A P
authors.initials: AP
authors.lastname: Semakova
authors.forename: L N
authors.initials: LN
authors.lastname: Novikova
authors.forename: A L
authors.initials: AL
authors.lastname: Kravtsov
authors.forename: S A
authors.initials: SA
authors.lastname: Bugorkova
authors.forename: T N
authors.initials: TN
authors.lastname: Shchukovskaia
authors.forename: Iu A
authors.initials: IuA
authors.lastname: Popov
authors.forename: V V
authors.initials: VV
authors.lastname: Kutyrev
mesh_terms: D000818:Animals; D022122:Anthrax Vaccines / Q000008:administration & dosage / Q000276:immunology*; D000942:Antigens, Bacterial / Q000276:immunology; D001408:Bacillus anthracis / Q000276:immunology*; D006168:Guinea Pigs; D006801:Humans; D007114:Immunization; D051379:Mice; D023421:Models, Animal; D011817:Rabbits; D014611:Vaccination; D014613:Vaccines, Attenuated / Q000008:administration & dosage / Q000276:immunology*
chemical_list: D022122:Anthrax Vaccines; D000942:Antigens, Bacterial; D014613:Vaccines, Attenuated