title: Association of the interleukin-22 genetic polymorphisms with ulcerative colitis.
abstract: BACKGROUND
  Interleukin-22 (IL-22) is a member of the IL-10 family of anti-inflammatory cytokines that mediates epithelial immunity. IL-22 expression was found to be increased in patients with ulcerative colitis (UC). Whether genetic polymorphisms of IL-22 also influence UC risk is still unknown. The purpose of this study was to investigate the association between the IL-22 gene polymorphisms (-429 C/T, +1046 T/A and +1995 A/C) and the risk of UC in Chinese Han patients.
  
  
  METHODS
  This hospital-based case-control study comprised 180 patients with UC and 180 age- and gender-matched controls. Genotypes of 3 common polymorphisms of the IL-22 gene were determined by fluorogenic 5' exonuclease assays (TaqMan).
  
  
  RESULTS
  Patients with UC had a significantly higher frequency of IL-22 -429 TT genotype [odds ratio (OR) =2.43, 95% confidence interval (CI) =1.35, 4.37; P=0.003] and -429 T allele (OR =1.54, 95% CI=1.14, 2.07; P=0.004) than controls. The findings are still emphatic by the Bonferroni correction. The IL-22+1046 T/A and IL-22+1995 A/C gene polymorphisms were not associated with a risk of UC. When stratifying by clinical type, location and disease severity of UC, no significant differences were found in any groups.
  
  
  CONCLUSIONS
  This is the first study to provide evidence for an association of IL-22 -429 C/T gene polymorphisms with UC risk. Additional well-designed large studies were required for the validation of our results.
  The virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/13000_2014_183.
authors.forename: Hong Gang
authors.initials: HG
authors.lastname: Chi
authors.forename: Xue Bao
authors.initials: XB
authors.lastname: Zheng
authors.forename: Zhu Guo
authors.initials: ZG
authors.lastname: Wu
authors.forename: Shi Xue
authors.initials: SX
authors.lastname: Dai
authors.forename: Zheng
authors.initials: Z
authors.lastname: Wan
authors.affiliation: Department of Traditional Chinese Medicine, The Second Clinical Medical College, Guangdong Medical College, 1 Xincheng Road, Songshan LakeSci,&Tech, Industry Park, Dongguan 523808, Guangdong, China. yzou98@hotmail.com.
authors.forename: Ying
authors.initials: Y
authors.lastname: Zou
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D003093:Colitis, Ulcerative / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D007378:Interleukins / Q000235:genetics*; D008297:Male; D016017:Odds Ratio; D020641:Polymorphism, Single Nucleotide; D000097043:Interleukin-22
chemical_list: D007378:Interleukins; D000097043:Interleukin-22