title: The production of monoclonal antibodies from human lymphocytes.
abstract: Two conceptually unique approaches are currently available for the production of human monoclonal antibodies - the 'hybridoma' technique, based on the fusion of antibody-producing B lymphocytes with plasmacytoma cells or lymphoblastoid cell lines; and the use of Epstein-Barr virus (EBV) to 'immortalize' antigen-specific, human B lymphocytes. Here Danuta Kozbor and, John Roder discuss the contribution each technique is making, alone or in combination, towards the desired aim of producing human monoclonal antibodies with a defined specificity.
authors.affiliation: Department of Microbiology and Immunology, Queen's University, Kingston, Ontario, Canada K7L 3N6.
authors.forename: D
authors.initials: D
authors.lastname: Kozbor
authors.forename: J C
authors.initials: JC
authors.lastname: Roder