title: Spotlight on the in vitro effect of artemisinin-naphthoquine phosphate on Schistosoma mansoni and its snail host Biomphalaria alexandrina.
abstract: Malaria and schistosomiasis are the two most important parasitic diseases in the tropics and sub-tropics with geographic overlap. Efforts have been made for developing new schistosomicidal drugs, or testing existing drugs originally used for non-related diseases. The antimalarial artemisinin-naphthoquine phosphate combination (CO-ArNp) was recently reported to be a promising novel antischistosomal therapy with potent in vivo activity against Schistosoma mansoni. In this work, we report the in vitro dose- and time-response effect of CO-ArNp against the Egyptian strain of S. mansoni, and its snail host, Biomphalaria alexandrina. Incubation of adult S. mansoni with CO-ArNp at 40 or 20 μg/ml for 48 or 72 h killed all worms. Exposure of S. mansoni miracidia and cercariae to the molluscicidal LC50 of CO-ArNp (16.8 μg/ml) resulted in 100% mortality of the free larval stages within 90 and 15 min, respectively. Moreover, incubation of adult B. alexandrina snails with this drug combination killed all snails at 40 μg/ml within 24h. Scanning electron microscope revealed marked morphological and tegumental alterations on the different stages of the parasite and its snail soft tissue. Our study highlights the schistosomicidal and molluscicidal effects of artemisinin-naphthoquine phosphate. No doubt more studies are needed to clarify its potential value to control schistosomiasis.
authors.affiliation: Department of Medical Parasitology, Faculty of Medicine, Mansoura University, 2 El-Gomhouria Street, Mansoura 35516, Egypt. Electronic address: selbeshbishi@yahoo.com.
authors.forename: Samar N
authors.initials: SN
authors.lastname: El-Beshbishi
authors.affiliation: Department of Medical Malacology, Theodor Bilharz Research Institute, Warrak El Hadar, Imbaba, Giza, Egypt.
authors.forename: Samia
authors.initials: S
authors.lastname: El Bardicy
authors.affiliation: Department of Medical Malacology, Theodor Bilharz Research Institute, Warrak El Hadar, Imbaba, Giza, Egypt.
authors.forename: Menerva
authors.initials: M
authors.lastname: Tadros
authors.affiliation: Department of Medical Malacology, Theodor Bilharz Research Institute, Warrak El Hadar, Imbaba, Giza, Egypt.
authors.forename: Magda
authors.initials: M
authors.lastname: Ayoub
authors.affiliation: Department of Medical Parasitology, Faculty of Medicine, Mansoura University, 2 El-Gomhouria Street, Mansoura 35516, Egypt.
authors.forename: Amira
authors.initials: A
authors.lastname: Taman
mesh_terms: D000818:Animals; D037621:Artemisinins / Q000494:pharmacology*; D001700:Biomphalaria / Q000187:drug effects* / Q000469:parasitology / Q000648:ultrastructure; D058487:Cercaria / Q000187:drug effects*; D066298:In Vitro Techniques; D008855:Microscopy, Electron, Scanning; D009285:Naphthoquinones / Q000494:pharmacology*; D012550:Schistosoma mansoni / Q000187:drug effects* / Q000648:ultrastructure; D012556:Schistosomicides / Q000494:pharmacology*
chemical_list: D037621:Artemisinins; D009285:Naphthoquinones; D012556:Schistosomicides; C543979:artemisinin-naphthoquine combination
keywords: Artemisinin; Biomphalaria alexandrina; In vitro; Naphthoquine phosphate; Scanning electron microscope; Schistosoma mansoni