title: Regulation of the hydrolytic activity of Mg²⁺-dependent phosphatase models by intramolecular NH···O hydrogen bonds.
abstract: Magnesium-dependent phosphatase models containing intramolecular NH···O hydrogen bonds were synthesized and structurally characterized by X-ray analysis. The Mg-O bond distances varied with the mode of the hydrogen bonds. (1)H NMR spectra in nonpolar solvents revealed that the acidity of the coordinated water molecule was regulated by the hydrogen bonds. Further, stoichiometric hydrolysis of phosphoric ester significantly depended on the hydrogen bonds. Zinc analogues showed similar but smaller dependencies, which suggest the indispensable role of Mg(2+) ion in the activation of the enzymes.
authors.affiliation: Department of Macromolecular Science, Graduate School of Science, Osaka University , Toyonaka, Osaka 560-0043, Japan.
authors.forename: Taka-aki
authors.initials: TA
authors.lastname: Okamura
authors.forename: Ryosuke
authors.initials: R
authors.lastname: Furuya
authors.forename: Kiyotaka
authors.initials: K
authors.lastname: Onitsuka
mesh_terms: D006860:Hydrogen Bonding; D006868:Hydrolysis; D008274:Magnesium / Q000378:metabolism*; D008958:Models, Molecular; D010744:Phosphoric Monoester Hydrolases / Q000737:chemistry* / Q000378:metabolism*
chemical_list: D010744:Phosphoric Monoester Hydrolases; D008274:Magnesium