title: Mechanism of mast cell adhesion to human tenocytes in vitro.
abstract: Mast cells and fibroblasts are two key players involved in many fibrotic and degenerative disorders. In the present study we examined the nature of binding interactions between human mast cells and tendon fibroblasts (tenocytes). In the mast cell-fibroblast co-culture model, mast cells were shown to spontaneously bind to tenocytes, in a process that was partially mediated by α5β1 integrin receptors. The same receptors on mast cells significantly mediated binding of these cells to tissue culture plates in the presence of tenocyte-conditioned media; the tenocyte-derived fibronectin in the media was shown to also play a major role in these binding activities. Upon binding to tenocytes or tissue culture plates, mast cells acquired an elongated phenotype, which was dependent on α5β1 integrin and tenocyte fibronectin. Additionally, tenocyte-derived fibronectin significantly enhanced mRNA expression of the adhesion molecule, THY1, by mast cells. Our data suggests that α5β1 integrin mediates binding of mast cells to human tenocyte and to tenocyte-derived ECM proteins, in particular fibronectin.
authors.affiliation: Faculty of Medicine, University of British Columbia, Vancouver, Canada; Vancouver Coastal Health Research Institute, Vancouver, Canada.
authors.forename: Hayedeh
authors.initials: H
authors.lastname: Behzad
authors.forename: Shu-Huei
authors.initials: SH
authors.lastname: Tsai
authors.forename: Paulina
authors.initials: P
authors.lastname: Nassab
authors.forename: Rouhollah
authors.initials: R
authors.lastname: Mousavizadeh
authors.forename: Robert G
authors.initials: RG
authors.lastname: McCormack
authors.forename: Alex
authors.initials: A
authors.lastname: Scott
mesh_terms: D002448:Cell Adhesion / Q000502:physiology; D002450:Cell Communication / Q000502:physiology*; D002460:Cell Line; D002478:Cells, Cultured; D018920:Coculture Techniques; D005109:Extracellular Matrix / Q000502:physiology; D005260:Female; D005347:Fibroblasts / Q000166:cytology* / Q000502:physiology; D005353:Fibronectins / Q000502:physiology; D006801:Humans; D066298:In Vitro Techniques; D039081:Integrin alpha5beta1 / Q000502:physiology; D008297:Male; D008407:Mast Cells / Q000166:cytology* / Q000502:physiology; D019009:Proto-Oncogene Proteins c-kit / Q000502:physiology; D013710:Tendons / Q000166:cytology* / Q000502:physiology; D018800:Thy-1 Antigens / Q000502:physiology
chemical_list: D005353:Fibronectins; D039081:Integrin alpha5beta1; D018800:Thy-1 Antigens; D019009:Proto-Oncogene Proteins c-kit
keywords: fibroblasts; fibronectin; mast cells; tenocytes; α5β1