title: Probing plasmodesmata function with biochemical inhibitors.
abstract: To investigate plasmodesmata (PD) function, a useful technique is to monitor the effect on cell-to-cell transport of applying an inhibitor of a physiological process, protein, or other cell component of interest. Changes in PD transport can then be monitored in one of several ways, most commonly by measuring the cell-to-cell movement of fluorescent tracer dyes or of free fluorescent proteins. Effects on PD structure can be detected in thin sections of embedded tissue observed using an electron microscope, most commonly a Transmission Electron Microscope (TEM). This chapter outlines commonly used inhibitors, methods for treating different tissues, how to detect altered cell-to-cell transport and PD structure, and important caveats.
authors.affiliation: Black Mountain Laboratories, CSIRO Plant Industry, GPO Box 1600, Clunies Ross Street, Black Mountain, ACT, 2601, Australia, rosemary.white@csiro.au.
authors.forename: Rosemary G
authors.initials: RG
authors.lastname: White
mesh_terms: D000199:Actins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D017360:Arabidopsis / Q000187:drug effects / Q000502:physiology* / Q000648:ultrastructure; D001692:Biological Transport; D019086:Bridged Bicyclo Compounds, Heterocyclic / Q000494:pharmacology; D003571:Cytochalasin B / Q000494:pharmacology; D003603:Cytotoxins / Q000494:pharmacology*; D047630:Depsipeptides / Q000494:pharmacology; D005404:Fixatives / Q000737:chemistry; D005456:Fluorescent Dyes / Q000032:analysis / Q000378:metabolism; D015870:Gene Expression; D049452:Green Fluorescent Proteins / Q000032:analysis / Q000235:genetics; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D008845:Microinjections; D046529:Microscopy, Electron, Transmission; D008856:Microscopy, Fluorescence; D008867:Microtomy; D010590:Phalloidine / Q000494:pharmacology; D018517:Plant Roots / Q000187:drug effects / Q000502:physiology* / Q000648:ultrastructure; D031425:Plasmodesmata / Q000187:drug effects / Q000502:physiology* / Q000648:ultrastructure; D051304:Profilins / Q000494:pharmacology; D011993:Recombinant Fusion Proteins / Q000032:analysis / Q000235:genetics; D053778:Thiazolidines / Q000494:pharmacology; D016707:Tissue Fixation; D034823:Tradescantia / Q000187:drug effects / Q000502:physiology* / Q000648:ultrastructure
chemical_list: D000199:Actins; D019086:Bridged Bicyclo Compounds, Heterocyclic; D003603:Cytotoxins; D047630:Depsipeptides; D005404:Fixatives; D005456:Fluorescent Dyes; D051304:Profilins; D011993:Recombinant Fusion Proteins; D053778:Thiazolidines; C057531:jasplakinolide; D049452:Green Fluorescent Proteins; D010590:Phalloidine; D003571:Cytochalasin B; C037068:latrunculin B; C037067:latrunculin A