title: Finger millet [Eleusine coracana (L.) Gaertn].
abstract: Millets are the primary food source for millions of people in tropical regions of the world supplying mineral nutrition and protein. In this chapter, we describe an optimized protocol for the Agrobacterium-mediated transformation of finger millet variety GPU 45. Agrobacterium strain LBA4404 harboring plasmid pCAMBIA1301 which contains hygromycin phosphotransferase (hph) as selectable marker gene and β-glucuronidase (GUS) as reporter gene has been used. This protocol utilizes the shoot apex explants for the somatic embryogenesis and regeneration of finger millet after the transformation by Agrobacterium. Desiccation of explants during cocultivation helps for the better recovery of transgenic plants. This protocol is very useful for the efficient production of transgenic plants in finger millet through Agrobacterium-mediated transformation.
authors.affiliation: Centre for Plant Sciences, Faculty of Biological Sciences, University of Leeds, Leeds, LS2 9JT, UK.
authors.forename: Stanislaus Antony
authors.initials: SA
authors.lastname: Ceasar
authors.forename: Savarimuthu
authors.initials: S
authors.lastname: Ignacimuthu
mesh_terms: D000383:Agriculture / Q000379:methods; D060054:Agrobacterium / Q000235:genetics; D018920:Coculture Techniques; D031748:Eleusine / Q000235:genetics* / Q000254:growth & development; D017930:Genes, Reporter; D005821:Genetic Techniques; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000235:genetics; D030821:Plants, Genetically Modified; D010957:Plasmids; D014170:Transformation, Genetic
chemical_list: D017853:Phosphotransferases (Alcohol Group Acceptor); C063242:hygromycin-B kinase