title: The temporal expression of estrogen receptor alpha-36 and runx2 in human bone marrow derived stromal cells during osteogenesis.
abstract: During bone maintenance in vivo, estrogen signals through estrogen receptor (ER)-α. The objectives of this study were to investigate the temporal expression of ERα36 and ascertain its functional relevance during osteogenesis in human bone marrow derived stromal cells (BMSC). This was assessed in relation to runt-related transcription factor-2 (runx2), a main modulatory protein involved in bone formation. ERα36 and runx2 subcellular localisation was assessed using immunocytochemistry, and their mRNA expression levels by real time PCR throughout the process of osteogenesis. The osteogenically induced BMSCs demonstrated a rise in ERα36 mRNA during proliferation followed by a decline in expression at day 10, which represents a change in dynamics within the culture between the proliferative stage and the differentiative stage. The mRNA expression profile of runx2 mirrored that of ERα36 and showed a degree subcellular co-localisation with ERα36. This study suggests that ERα36 is involved in the process of osteogenesis in BMSCs, which has implications in estrogen deficient environments.
authors.affiliation: Institute of Life Science, College of Medicine, Swansea University, UK. Electronic address: w.francis@swansea.ac.uk.
authors.forename: W R
authors.initials: WR
authors.lastname: Francis
authors.affiliation: Institute of Life Science, College of Medicine, Swansea University, UK.
authors.forename: S E
authors.initials: SE
authors.lastname: Owens
authors.affiliation: Institute of Life Science, College of Medicine, Swansea University, UK.
authors.forename: C
authors.initials: C
authors.lastname: Wilde
authors.affiliation: Institute of Life Science, College of Medicine, Swansea University, UK; Trauma & Orthopaedics, Morriston Hospital, Swansea, UK.
authors.forename: I
authors.initials: I
authors.lastname: Pallister
authors.affiliation: Institute of Life Science, College of Medicine, Swansea University, UK.
authors.forename: V
authors.initials: V
authors.lastname: Kanamarlapudi
authors.affiliation: College of Life Sciences, Liaoning Normal University, Dalian 116081, China; Liaoning Key Laboratories of Biotechnology and Molecular Drug Research & Development, Dalian 116081, China. Electronic address: weizou60@hotmail.com.
authors.forename: W
authors.initials: W
authors.lastname: Zou
authors.affiliation: Institute of Life Science, College of Medicine, Swansea University, UK.
authors.forename: Z
authors.initials: Z
authors.lastname: Xia
mesh_terms: D001483:Base Sequence; D050659:Core Binding Factor Alpha 1 Subunit / Q000235:genetics / Q000378:metabolism*; D017931:DNA Primers; D047628:Estrogen Receptor alpha / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000378:metabolism*; D010012:Osteogenesis; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D050659:Core Binding Factor Alpha 1 Subunit; D017931:DNA Primers; D047628:Estrogen Receptor alpha; D012333:RNA, Messenger; C493709:RUNX2 protein, human
keywords: Bone marrow derived stromal cells (BMSC); Estrogen receptor (ER); Osteogenesis; Runt-related transcription factor-2 (runx2)