title: Human mesenchymal stem cells produce bioactive neurotrophic factors: source, individual variability and differentiation issues.
abstract: The possible use of cell therapies for neurological lesions and disorders is regarded as a very promising strategy. However, many issues related to cell type, tissue donor, expected biological action etc., are still open. In this study human mesenchymal stem cells derived from different fetal and adult tissues were examined in order to explore growth and neurotrophic factor synthesis and biological action, also considering the individual variability of the donors. Cells were derived from different human tissues and characterized according to the guidelines of the International Society for Cellular Therapy. Growth and neurotrophic factor synthesis was evaluated by real time PCR, biological assays and ELISA. It was found that human mesenchymal stem cells produce vascular endothelial-, nerve-growth factor (VEGF, NGF), brain-derived-, ciliary- and glial-derived neurotrophic factors (BDNF, CDGF, GDNF), which are neuroprotective molecules, but the source and the donor influence the synthesis rate. Accordingly, it is suggested that the source and the individual variability are key issues to be considered in the perspective of the clinical use of mesenchymal stem cells in neurological disorders.
authors.affiliation: Department of Veterinary Medical Sciences, University of Bologna, Bologna, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Paradisi
authors.affiliation: Department of Experimental, Diagnostic and Specialty Medicine, Unit of Histology, Embryology and Applied Biology, University of Bologna, Bologna, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Alviano
authors.affiliation: Department of Veterinary Medical Sciences, University of Bologna, Bologna, Italy.
authors.forename: S
authors.initials: S
authors.lastname: Pirondi
authors.affiliation: Department of Experimental, Diagnostic and Specialty Medicine, Unit of Histology, Embryology and Applied Biology, University of Bologna, Bologna, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Lanzoni
authors.affiliation: Health Science and Technology Interdepartmental Center for Industrial Research (HST-ICIR), University of Bologna, Bologna, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Fernandez
authors.affiliation: Department of Veterinary Medical Sciences, University of Bologna, Bologna, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Lizzo
authors.affiliation: Department of Veterinary Medical Sciences, University of Bologna, Bologna, Italy.
authors.forename: L
authors.initials: L
authors.lastname: Giardino
authors.affiliation: Department of Veterinary Medical Sciences, University of Bologna, Bologna, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Giuliani
authors.affiliation: Department of Experimental, Diagnostic and Specialty Medicine, Unit of Histology, Embryology and Applied Biology, University of Bologna, Bologna, Italy.
authors.forename: R
authors.initials: R
authors.lastname: Costa
authors.affiliation: Department of Experimental, Diagnostic and Specialty Medicine, Unit of Histology, Embryology and Applied Biology, University of Bologna, Bologna, Italy.
authors.forename: C
authors.initials: C
authors.lastname: Marchionni
authors.affiliation: Department of Experimental, Diagnostic and Specialty Medicine, Unit of Histology, Embryology and Applied Biology, University of Bologna, Bologna, Italy.
authors.forename: L
authors.initials: L
authors.lastname: Bonsi
authors.affiliation: Department of Veterinary Medical Sciences, University of Bologna, Bologna, Italy.
authors.forename: L
authors.initials: L
authors.lastname: Calza
mesh_terms: D002454:Cell Differentiation; D002469:Cell Separation; D006801:Humans; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D009414:Nerve Growth Factors / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis
chemical_list: D009414:Nerve Growth Factors; D012333:RNA, Messenger