title: Isolation and characterization of simple sequence repeat markers for the herbaceous species Phyla scaberrima (Verbenaceae).
abstract: Phyla scaberrima (Verbenaceae) is a herbaceous species distributed from Mexico to Panama. Because of its well-known sweet properties and other medicinal uses, this species is cultivated in South America and the Caribbean. Phyla scaberrima has been arbitrarily extracted from nature, resulting in a severe reduction in its gene pool. In this study, we developed and characterized 11 simple sequence repeat markers for P. scaberrima to determine the genetic variability and patterns of population structure of the species. Fifty-six alleles were detected in a sample of 48 individuals belonging to 3 different populations. The average number of alleles per locus was 5.09, while the polymorphic information content ranged from 0.000-0.587. The observed and expected heterozygosities varied from 0.000-0.543 and from 0.000-0.651, respectively. Two loci exhibited significant deviation of the expected Hardy-Weinberg proportion. The 11 primer pairs were also tested for cross-amplification to 6 species of the related genus Lippia. The transferability rate ranged from 4 loci in Lippia florida and L. rotundifolia to 6 loci in L. corymbosa and L. microcephala. The 11 primer sets were shown to be valuable tools for population genetic studies in P. scaberrima and in species of the genus Lippia in which primer transferability was detected.
authors.affiliation: Departamento de Agronomia, Centro de Ciências Agrárias, Universidade Estadual de Londrina, Londrina, PR, Brasil.
authors.forename: C L
authors.initials: CL
authors.lastname: Chaves
authors.affiliation: Departamento de Biologia Geral, Centro de Ciências Biológicas, Universidade Estadual de Londrina, Londrina, PR, Brasil.
authors.forename: E A
authors.initials: EA
authors.lastname: Ruas
authors.affiliation: Departamento de Biologia Geral, Centro de Ciências Biológicas, Universidade Estadual de Londrina, Londrina, PR, Brasil.
authors.forename: C F
authors.initials: CF
authors.lastname: Ruas
authors.affiliation: Departamento de Agronomia, Centro de Ciências Agrárias, Universidade Estadual de Londrina, Londrina, PR, Brasil.
authors.forename: J
authors.initials: J
authors.lastname: Delfini
authors.affiliation: Departamento de Agronomia, Centro de Ciências Agrárias, Universidade Estadual de Londrina, Londrina, PR, Brasil.
authors.forename: N C
authors.initials: NC
authors.lastname: Bejatto
authors.affiliation: Departamento de Agronomia, Centro de Ciências Agrárias, Universidade Estadual de Londrina, Londrina, PR, Brasil.
authors.forename: B D
authors.initials: BD
authors.lastname: Góes
authors.affiliation: Departamento de Biologia Geral, Centro de Ciências Biológicas, Universidade Estadual de Londrina, Londrina, PR, Brasil ruas@uel.br.
authors.forename: P M
authors.initials: PM
authors.lastname: Ruas
mesh_terms: D001483:Base Sequence; D017931:DNA Primers; D017343:Genes, Plant; D005819:Genetic Markers; D018895:Microsatellite Repeats; D016133:Polymerase Chain Reaction; D029648:Verbenaceae / Q000235:genetics*
chemical_list: D017931:DNA Primers; D005819:Genetic Markers