title: In vitro genotoxicity of mycotoxins ochratoxin A and fumonisin B(1) could be prevented by sodium copper chlorophyllin--implication to their genotoxic mechanism.
abstract: The aim of this study was to investigate the possible protective effect of sodium copper chlorophyllin (CHL) against cytotoxicity and DNA damage induced by mycotoxins ochratoxin A (OTA) and fumonisin B1 (FB1). CHL (0.1-100 μg/ml) alone had no impact on cell viability and genome damage in the primary human peripheral blood lymphocytes (HPBLs) and exhibited free radical scavenging activity in the DPPH assay. Both mycotoxins, OTA (4 μmol/l) and FB1 (20 μg/ml), induced DNA damage in HPBLs already after 1 h exposure. When the HPBLs were co-exposed to CHL (10 and 100 μg/ml) and OTA (4 μmol/l) or FB1 (20 μg/ml) for 1 h, CHL protected against cell and DNA damage induced by both mycotoxins, implying that OTA and FB1 cytogenotoxicity mechanisms function at least partially through oxidative stress. Therefore, CHL could be a perfect candidate for possible use as an antioxidant.
authors.affiliation: Department of Pharmaceutical Botany, Faculty of Pharmacy and Biochemistry, University of Zagreb, A. Kovačića 1, Zagreb, Croatia. Electronic address: adomijan@pharma.hr.
authors.forename: Ana-Marija
authors.initials: AM
authors.lastname: Domijan
authors.affiliation: Mutagenesis Unit, Institute for Medical Research and Occupational Health, Ksaverska c. 2, Zagreb, Croatia.
authors.forename: Goran
authors.initials: G
authors.lastname: Gajski
authors.affiliation: Toxicology Unit, Institute for Medical Research and Occupational Health, Ksaverska c. 2, Zagreb, Croatia.
authors.forename: Ivana
authors.initials: I
authors.lastname: Novak Jovanović
authors.affiliation: Mutagenesis Unit, Institute for Medical Research and Occupational Health, Ksaverska c. 2, Zagreb, Croatia.
authors.forename: Marko
authors.initials: M
authors.lastname: Gerić
authors.affiliation: Mutagenesis Unit, Institute for Medical Research and Occupational Health, Ksaverska c. 2, Zagreb, Croatia.
authors.forename: Vera
authors.initials: V
authors.lastname: Garaj-Vrhovac
mesh_terms: D000328:Adult; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D002735:Chlorophyllides / Q000737:chemistry / Q000494:pharmacology*; D004249:DNA Damage / Q000187:drug effects*; D005260:Female; D016166:Free Radical Scavengers / Q000737:chemistry; D037341:Fumonisins / Q000633:toxicity*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000187:drug effects; D009793:Ochratoxins / Q000633:toxicity*; D018384:Oxidative Stress / Q000187:drug effects; D020011:Protective Agents / Q000737:chemistry / Q000494:pharmacology*; D018675:Toxicity Tests
chemical_list: D002735:Chlorophyllides; D016166:Free Radical Scavengers; D037341:Fumonisins; D009793:Ochratoxins; D020011:Protective Agents; C025589:ochratoxin A; C056933:fumonisin B1; C007020:chlorophyllin
keywords: Chlorophyllin; DNA damage; Human lymphocytes; Mycotoxins; Oxidative stress; Protective effect