title: Cytochrome P450-Mediated Phytoremediation using Transgenic Plants: A Need for Engineered Cytochrome P450 Enzymes.
abstract: There is an increasing demand for versatile and ubiquitous Cytochrome P450 (CYP) biocatalysts for biotechnology, medicine, and bioremediation. In the last decade there has been an increase in realization of the power of CYP biocatalysts for detoxification of soil and water contaminants using transgenic plants. However, the major limitations of mammalian CYP enzymes are that they require CYP reductase (CPR) for their activity, and they show relatively low activity, stability, and expression. On the other hand, bacterial CYP enzymes show limited substrate diversity and usually do not metabolize herbicides and industrial contaminants. Therefore, there has been a considerable interest for biotechnological industries and the scientific community to design CYP enzymes to improve their catalytic efficiency, stability, expression, substrate diversity, and the suitability of P450-CPR fusion enzymes. Engineered CYP enzymes have potential for transgenic plants-mediated phytoremediation of herbicides and environmental contaminants. In this review we discuss: 1) the role of CYP enzymes in phytoremediation using transgenic plants, 2) problems associated with wild-type CYP enzymes in phytoremediation, and 3) examples of engineered CYP enzymes and their potential role in transgenic plant-mediated phytoremediation.
authors.affiliation: School of Pharmacy, University of Missouri, USA.
authors.forename: Santosh
authors.initials: S
authors.lastname: Kumar
authors.affiliation: School of Pharmacy, University of Missouri, USA.
authors.forename: Mengyao
authors.initials: M
authors.lastname: Jin
authors.affiliation: School of Pharmacy, University of Missouri, USA.
authors.forename: James L
authors.initials: JL
authors.lastname: Weemhoff
keywords: Cytochrome P450; P450 engineering; Phytoremediation; Transgenic plants