title: Therapeutic enhancement of newly derived bacteriocins against Giardia lamblia.
abstract: Trials for identifying efficient anti-giardial agents are still ongoing. Nowadays, bacteriocins have attracted the attention as potential antimicrobial compounds. For the first time, the current study evaluated the therapeutic efficacy of bacteriocins derived from newly isolated Egyptian strains of probiotics Lactobacilli; L. acidophilus (P106) and L. plantarum (P164) against Giardia lamblia. Bacteriocins' efficacy was evaluated both in vitro; by growth inhibition and adherence assays, and in vivo; through estimation of parasite density, intestinal histopathological examination and ultrastructural analysis of Giardia trophozoites. In vivo bacteriocins' clinical safety was assessed. In vitro results proved that 50 µg of L. acidophilus bacteriocin induced reduction of the mean Giardia lamblia trophozoites by 58.3 ± 4.04%, while at lower concentrations of 10 and 20 µg of both L. acidophilus and L. plantarum, non significant reduction of the mean parasite density was achieved. In vitro trophozoites adherence was susceptible to the tested bacteriocins at all studied concentrations with variable degrees, while the highest adherence reduction was demonstrated using 50 µg of L acidophilus bacteriocin. In vivo, oral inoculation of 50 µg/mouse L. acidophilus bacteriocin for 5 successive days resulted in a noteworthy decline of the intestinal parasite density, along with amelioration of intestinal pathology of infected mice. Ultrastructural examination proved thatfive doses of L. acidophilus bacteriocin showed marked changes in cellular architecture of the trophozoites with evident disorganization of the cell membrane, adhesive disc and cytoplasmic components. This is the first reported study of the safe anti-giardial efficacy of L. acidophilus (P106) derived bacteriocin, hence highlighting its great promise as a potential therapeutic safe alternative to existing commercial drugs.
authors.affiliation: Medical Parasitology Department, Faculty of Medicine, Alexandria University, Egypt. Electronic address: ehabaman2003@yahoo.com.
authors.forename: Eglal I
authors.initials: EI
authors.lastname: Amer
authors.affiliation: Medical Parasitology Department, Faculty of Medicine, Alexandria University, Egypt.
authors.forename: Shereen F
authors.initials: SF
authors.lastname: Mossallam
authors.affiliation: Industrial Biotechnology Department, Genetic Engineering and Biotechnology Research Institute, Sadat City University, Egypt.
authors.forename: Hoda
authors.initials: H
authors.lastname: Mahrous
mesh_terms: D000818:Animals; D001430:Bacteriocins / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*; D002448:Cell Adhesion / Q000187:drug effects; D005260:Female; D016829:Giardia lamblia / Q000187:drug effects* / Q000254:growth & development / Q000648:ultrastructure; D005873:Giardiasis / Q000188:drug therapy*; D006801:Humans; D007413:Intestinal Mucosa / Q000469:parasitology / Q000473:pathology; D007421:Intestine, Small / Q000469:parasitology / Q000473:pathology; D007779:Lactobacillus acidophilus / Q000737:chemistry*; D048191:Lactiplantibacillus plantarum / Q000737:chemistry*; D008297:Male; D051379:Mice; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D019936:Probiotics / Q000737:chemistry
chemical_list: D001430:Bacteriocins
keywords: Bacteriocin; Giardia lamblia; In vitro; In vivo; Lactobacillus acidophilus; Lactobacillus plantarum