title: Anticancer Active Homoisoflavone from the Underground Bulbs of Ledebouria hyderabadensis.
abstract: BACKGROUND
  Ledebouria is a genus of deciduous or weakly evergreen bulbs in the Hyacinthaceae family. This is recognized as the first collection made of the new taxon Ledebouria hyderabadensis, exist in the Hyderabad city of Andhra Pradesh, India.
  
  
  OBJECTIVE
  The goal of this work was to investigate the phytochemical constituents present in the new specifies and also to evaluate the cytotoxic properties of the extracts and pure compounds against human cancer cell lines.
  
  
  METHODS
  The anticancer activity was evaluated in in vitro mode by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) test.
  
  
  RESULTS
  Phytochemical investigation of underground bulbs of indigenous, rare, and recently identified herb L. hyderabadensis yielded a bioactive homoisoflavanone, Scillascillin 1. The structure of the compound was established on the basis of various nuclear magnetic resonance and mass spectral data. The compound Scillascillin was isolated for the first time from L. hyderabadensis. In vitro anticancer activity, performed using MTT assay, showed compound 1 as significantly active against human cancer cell lines MCF-7 (breast cancer) and DU-145 (prostate cancer) with inhibitory concentration (IC)50 values 9.59 and 11.32 μg/ml respectively when compared with herb methanol extract (IC50 values 36.21 and 44.86 μg/ml respectively).
authors.affiliation: CSIR-Central Institute of Medicinal and Aromatic Plants, Research Centre, Natura Products Chemistry, Boduppal, Hyderabad, Andhra Pradesh, India.
authors.forename: Yakaiah
authors.initials: Y
authors.lastname: Chinthala
authors.affiliation: Toxicology Unit, Biology Division, CSIR-IICT, Tarnaka, Hyderabad, Andhra Pradesh, India.
authors.forename: Srinivas
authors.initials: S
authors.lastname: Chinde
authors.affiliation: CSIR-Central Institute of Medicinal and Aromatic Plants, Research Centre, Natura Products Chemistry, Boduppal, Hyderabad, Andhra Pradesh, India.
authors.forename: Arigari Niranjana
authors.initials: AN
authors.lastname: Kumar
authors.affiliation: CSIR-Central Institute of Medicinal and Aromatic Plants, Research Centre, Natura Products Chemistry, Boduppal, Hyderabad, Andhra Pradesh, India.
authors.forename: K V N Satya
authors.initials: KV
authors.lastname: Srinivas
authors.affiliation: CSIR-Central Institute of Medicinal and Aromatic Plants, Research Centre, Natura Products Chemistry, Boduppal, Hyderabad, Andhra Pradesh, India.
authors.forename: Jonnala Kotesh
authors.initials: JK
authors.lastname: Kumar
authors.affiliation: CSIR-Central Institute of Medicinal and Aromatic Plants, Research Centre, Natura Products Chemistry, Boduppal, Hyderabad, Andhra Pradesh, India.
authors.forename: Kakaraparthy Pandu
authors.initials: KP
authors.lastname: Sastry
authors.affiliation: Toxicology Unit, Biology Division, CSIR-IICT, Tarnaka, Hyderabad, Andhra Pradesh, India.
authors.forename: Paramjit
authors.initials: P
authors.lastname: Grover
authors.affiliation: Department of Botany, Nizam College, Osmania University, Hyderabad, Andhra Pradesh, India.
authors.forename: M Venkat
authors.initials: MV
authors.lastname: Ramana
keywords: Anticancer activity; Hyacinthaceae; Ledebouria hyderabadensis; Scillascillin