title: Polymorphism -433 C>T of the Osteopontin gene is associated with the susceptibility to develop gliomas and their prognosis in a Chinese cohort.
abstract: OBJECTIVE
  To investigate role of the Osteopontin (OPN) genetic polymorphisms in the susceptibility to gliomas and their prognosis.
  
  
  METHODS
  A total of 248 Chinese glioma patients and 281 age and sex matched healthy controls were recruited. The genetic polymorphisms at three loci, namely, -156 GG>G, -443 C>T and -66T>G, were determined. The log-rank test and Kaplan- Meier analysis were introduced to assess the effect of OPN gene polymorphisms on patient survival.
  
  
  RESULTS
  We found that the genotype frequencies of OPN -443 C>T polymorphism were significantly different between glioma patients and controls. Multivariable analyses showed a higher risk for gliomas in -443 CC genotype carriers compared to -443TT carriers (P<0.001). In addition, we also found the OPN -443 C>T polymorphism was closely related to the gliomas' tumor grade. The -443 C>T polymorphism also affected the tumor OPN expression level, but not the serum OPN level. More importantly, the -443 C>T polymorphism was significantly associated with the prognosis of these patients regardless of their treatment status. The patients with -443CC genotype had a poorer prognosis than those with -443TT and -443CT genotypes. In contrast, the -156 G>GG and -66T>G polymorphisms were not associated with risk, clinical characteristics, or prognosis of gliomas.
  
  
  CONCLUSIONS
  This study suggests that the -443C>T gene polymorphisms may be used as a molecular marker for glioma occurrence and clinical outcome in glioma patients.
authors.affiliation: Department of Neurosurgery, Children's Hospital, Zhejiang University School of Medicine, Hangzhou, P. R. China.
authors.forename: Zhipeng
authors.initials: Z
authors.lastname: Shen
authors.forename: Bo
authors.initials: B
authors.lastname: Chen
authors.forename: Xuwei
authors.initials: X
authors.lastname: Hou
authors.forename: Peng
authors.initials: P
authors.lastname: Chen
authors.forename: Guoqiang
authors.initials: G
authors.lastname: Zhao
authors.forename: Jiajie
authors.initials: J
authors.lastname: Fan
mesh_terms: D000328:Adult; D044466:Asian People / Q000235:genetics*; D016022:Case-Control Studies; D005260:Female; D020022:Genetic Predisposition to Disease / Q000235:genetics*; D005838:Genotype; D005910:Glioma / Q000235:genetics* / Q000473:pathology; D006801:Humans; D008297:Male; D008875:Middle Aged; D053495:Osteopontin / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D011379:Prognosis; D012306:Risk
chemical_list: D053495:Osteopontin