title: Further investigation in europeans of susceptibility variants for polycystic ovary syndrome discovered in genome-wide association studies of Chinese individuals.
abstract: BACKGROUND
  Two genome-wide association studies (GWAS) of polycystic ovary syndrome (PCOS) have identified 11 susceptibility loci in Chinese individuals. Some of the risk loci identified in Chinese cohorts, mostly from the first GWAS, have been replicated in Europeans. Replication of the loci from the second GWAS in European cohorts is necessary to determine whether the same variants confer risk for PCOS in multiple ethnicities.
  
  
  OBJECTIVE
  The objective of the study was to determine the effects of the Chinese GWAS loci in European-origin individuals.
  
  
  METHODS
  This was a genetic association study.
  
  
  METHODS
  The study was conducted at a tertiary care academic center.
  
  
  METHODS
  Eight hundred forty-five European subjects with PCOS and 845 controls participated in the study.
  
  
  METHODS
  INTERVENTIONS included blood sampling and genotyping.
  
  
  METHODS
  The association between PCOS and 12 independent single-nucleotide polymorphisms mapping to seven of the Chinese GWAS loci in a European cohort was measured.
  
  
  RESULTS
  Variants in DENND1A (P = .0002), THADA (P = .035), FSHR (P = .007), and INSR (P = .046) were associated with PCOS in Europeans. The genetic risk score, generated for each subject based on the total number of risk alleles, was associated with the diagnosis of PCOS (P < .0001) and remained associated (P = .02), even after exclusion of the four variants individually associated with PCOS.
  
  
  CONCLUSIONS
  At least four of the PCOS susceptibility loci identified in the Chinese GWAS are associated with PCOS in Europeans. The overall genetic burden for PCOS, as demonstrated by the risk score, is also associated with the diagnosis of PCOS in Europeans. The PCOS susceptibility loci identified in the Chinese GWAS are thus likely to play an important role in the etiology of PCOS across ethnicities.
authors.affiliation: Department of Obstetrics and Gynecology (M.A.B.), David Geffen School of Medicine at University of California, Los Angeles, Los Angeles, California 90095; Division of Endocrinology, Diabetes, and Metabolism (M.R.J., M.O.G.), Department of Medicine, Cedars-Sinai Medical Center, Los Angeles, California 90048; Institute for Translational Genomics and Population Sciences and Department of Pediatrics (J.I.R.), Los Angeles Biomedical Research Institute at Harbor-University of California, Los Angeles, Medical Center, Torrance, California 90502; Children's Hospital of Oakland Research Institute (R.M.K.), Oakland, California 94609; Department of Obstetrics and Gynecology (R.S.L.), Pennsylvania State College of Medicine, Hershey, Pennsylvania 17033; and Departments of Obstetrics and Gynecology and Medicine (R.A.), Medical College of Georgia, Georgia Regents University, Augusta, Georgia 30912.
authors.forename: Meredith A
authors.initials: MA
authors.lastname: Brower
authors.forename: Michelle R
authors.initials: MR
authors.lastname: Jones
authors.forename: Jerome I
authors.initials: JI
authors.lastname: Rotter
authors.forename: Ronald M
authors.initials: RM
authors.lastname: Krauss
authors.forename: Richard S
authors.initials: RS
authors.lastname: Legro
authors.forename: Ricardo
authors.initials: R
authors.lastname: Azziz
authors.forename: Mark O
authors.initials: MO
authors.lastname: Goodarzi
mesh_terms: D000328:Adult; D000483:Alleles; D015703:Antigens, CD / Q000235:genetics; D044466:Asian People / Q000235:genetics*; D053418:Death Domain Receptor Signaling Adaptor Proteins / Q000235:genetics; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D055106:Genome-Wide Association Study; D005838:Genotype; D020662:Guanine Nucleotide Exchange Factors / Q000235:genetics; D006801:Humans; D009363:Neoplasm Proteins / Q000235:genetics; D011085:Polycystic Ovary Syndrome / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D011972:Receptor, Insulin / Q000235:genetics; D011962:Receptors, FSH / Q000235:genetics; D044465:White People / Q000235:genetics*
chemical_list: D015703:Antigens, CD; C571984:DENND1A protein, human; D053418:Death Domain Receptor Signaling Adaptor Proteins; D020662:Guanine Nucleotide Exchange Factors; D009363:Neoplasm Proteins; D011962:Receptors, FSH; C477698:THADA protein, human; C435941:INSR protein, human; D011972:Receptor, Insulin