title: Enzymatic exploration of catalase from a nanoparticle producing and biodecolorizing algae Shewanella xiamenensis BC01.
abstract: Shewanella xiamenensis (SXM) was found to produce nanoparticles (NPs) under aerobic condition. The oxidoreductase enzymatic activities including of catalase, manganese peroxidase, laccase, NADH dehydrogenase, flavin reductase, azoreductase and Fe reductase are first investigated. Catalase showed the greatest enzymatic activity among all oxidoreductases in SXM, which with strong activities in multiple substrates of ABTS, guaiacol and 2,6-DMP. The optimum temperature, pH, concentrations of H2O2 and 2,6-DMP for this enzyme were found to be 65 °C, pH 4.0, 128.7 mM and 10 mM, respectively. Finally, from the kinetic parameters and structure simulation of catalase, implied that SXM would potentially apply in bioremediation, microbe fuel cells (MFCs) and nano-biotechnology based on its distinguished enzymatic system.
authors.affiliation: Department of Chemical Engineering, National Cheng Kung University, Tainan 70101, Taiwan; Department of Chemical and Biochemical Engineering, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China. Electronic address: yswu@mail.ncku.edu.tw.
authors.forename: I-Son
authors.initials: IS
authors.lastname: Ng
authors.affiliation: Department of Chemical and Biochemical Engineering, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China; Department of Chemical and Biological Engineering, Zhejiang University, Hangzhou 310027, China.
authors.forename: Fangxin
authors.initials: F
authors.lastname: Xu
authors.affiliation: Department of Chemical and Biochemical Engineering, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China.
authors.forename: Xia
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Chemical and Biochemical Engineering, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China.
authors.forename: Chiming
authors.initials: C
authors.lastname: Ye
mesh_terms: D020418:Algal Proteins / Q000737:chemistry / Q000378:metabolism; D000595:Amino Acid Sequence; D001673:Biodegradation, Environmental; D002374:Catalase / Q000378:metabolism*; D003116:Color; D004591:Electrophoresis, Polyacrylamide Gel; D007700:Kinetics; D008958:Models, Molecular; D008969:Molecular Sequence Data; D053758:Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D010802:Phylogeny; D020592:Shewanella / Q000201:enzymology*; D013379:Substrate Specificity
chemical_list: D020418:Algal Proteins; D002374:Catalase
keywords: Catalase; Enzymatic exploration; Nanoparticle; Shewanella xiamenensis; Zymogram