title: Prognostic significance of CD3+ tumor-infiltrating lymphocytes in patients with endometrial carcinoma.
abstract: OBJECTIVE
  The aim of the present study was to investigate tumor-infiltrating leukocytes in patients with endometrial carcinoma.
  
  
  METHODS
  Cluster of differentiation (CD)3(+), CD8(+) and C20(+) tumor-infiltrating lymphocytes (TILs) and CD68(+) tumor-associated macrophages (TAMs) were evaluated retrospectively by immunohistochemistry in tumor specimen from 124 patients with endometrial carcinoma.
  
  
  RESULTS
  A significant decrease of CD3(+) TILs and an increase of CD68(+) TAM count was associated with higher tumor stage. In patients with early-stage, high-risk tumors, low intraepithelial CD3(+) TIL counts were associated with significantly inferior survival. In multivariate analysis of patients with early-stage tumors, intraepithelial CD3(+) TIL counts were an independent predictor of survival.
  
  
  CONCLUSIONS
  In patients with endometrial carcinoma a decrease of intraepithelial CD3(+) TIL counts is associated with advanced stage and high risk group. Intraepithelial CD3(+) TIL counts are an independent predictor of survival in patients with early tumors.
authors.affiliation: The Fingerland Institute of Pathology, Charles University Medical School and Teaching Hospital, Hradec Králové, Czech Republic.
authors.forename: Petra
authors.initials: P
authors.lastname: Čermáková
authors.affiliation: Department of Oncology and Radiotherapy, Charles University Medical School and Teaching Hospital, Hradec Králové, Czech Republic Department of Oncology, Palacký University Medical School and Teaching Hospital, Olomouc, Czech Republic bohuslav.melichar@fnol.cz.
authors.forename: Bohuslav
authors.initials: B
authors.lastname: Melichar
authors.affiliation: The Fingerland Institute of Pathology, Charles University Medical School and Teaching Hospital, Hradec Králové, Czech Republic.
authors.forename: Markéta
authors.initials: M
authors.lastname: Tomšová
authors.affiliation: Department of Oncology and Radiotherapy, Charles University Medical School and Teaching Hospital, Hradec Králové, Czech Republic.
authors.forename: Zdeněk
authors.initials: Z
authors.lastname: Zoul
authors.affiliation: Department of Oncology, Palacký University Medical School and Teaching Hospital, Olomouc, Czech Republic.
authors.forename: Hana
authors.initials: H
authors.lastname: Kalábová
authors.affiliation: Department of Gynecology and Obstetrics, Charles University Medical School and Teaching Hospital, Hradec Králové, Czech Republic.
authors.forename: Jiří
authors.initials: J
authors.lastname: Spaček
authors.affiliation: Department of Oncology, Palacký University Medical School and Teaching Hospital, Olomouc, Czech Republic.
authors.forename: Martin
authors.initials: M
authors.lastname: Doležel
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D017252:CD3 Complex / Q000378:metabolism; D002452:Cell Count; D016889:Endometrial Neoplasms / Q000276:immunology* / Q000401:mortality / Q000473:pathology* / Q000601:surgery; D005260:Female; D006801:Humans; D007962:Leukocytes / Q000276:immunology / Q000473:pathology; D016246:Lymphocytes, Tumor-Infiltrating / Q000276:immunology* / Q000378:metabolism; D008875:Middle Aged; D060787:Neoplasm Grading; D009367:Neoplasm Staging; D011379:Prognosis
chemical_list: D017252:CD3 Complex
keywords: Biomarkers; endometrial carcinoma; tumor-associated macrophages; tumor-infiltrating lymphocytes