title: Assessment of Becton Dickinson Plain and Serum Separator Tubes in Measurement of 25-Hydroxyvitamin D3 (25OHD3) by HPLC and Immunoassay Methods.
abstract: BACKGROUND
  The accuracy of 25-hydroxyvitamin D3 (25OHD3) measurement on specimens collected into serum separator tubes (SSTs) has been questioned because of possible interference by the gel. Possible interference was investigated in SSTs from Becton Dickinson (BD).
  
  
  METHODS
  Blood specimens were collected simultaneously from 50 normal subjects into plain tubes and SSTs. 25OHD3 was assayed on serum using high performance liquid chromatography (Chromsystems), and Architect (Abbott) and Liaison (Diasorin) immunoassays.
  
  
  RESULTS
  There were no significant differences between 25OHD3 results (means ± SE, nmol/l) obtained from specimens collected into plain tubes and SSTs assayed by HPLC (39.0 ± 2.7 vs. 39.3 ± 2.7), Liaison (32.9 ± 2.2 vs. 32.8 ± 2.3), or Architect (43.1 ± 2.8 vs. 43.2 ± 2.8). In specimens collected into plain tubes and SSTs, 25OHD3 measurements by HPLC correlated significantly (P < 0.0001) with those from the Architect (r = 0.895, r = 0.908) and Liaison (r = 0.907, r = 0.913), respectively.
  
  
  CONCLUSIONS
  The gel in SSTs (BD) does not interfere with the measurement of 25OHD3 by HPLC or common immunoassays. This important finding may enable clinical laboratories to make cost savings by using SSTs without concerns about inaccuracy.
authors.affiliation: King Abdullah International Medical Research Center, King Saud bin Abdulaziz University for Health Sciences, Pathology King Abdulaziz Medical City, Jeddah, Saudi Arabia.
authors.forename: Anwar
authors.initials: A
authors.lastname: Borai
authors.affiliation: Department of Clinical Biochemistry, Faculty of Medicine and Nutrition and Food Research Unit, King Fahd Medical Research Center, Jeddah, Saudi Arabia.
authors.forename: Suhad
authors.initials: S
authors.lastname: Bahijri
authors.affiliation: Department of Clinical Biochemistry, Royal Surrey County Hospital NHS Trust, Guildford, UK.
authors.forename: Callum
authors.initials: C
authors.lastname: Livingstone
authors.affiliation: King Abdullah International Medical Research Center, King Saud bin Abdulaziz University for Health Sciences, Pathology King Abdulaziz Medical City, Jeddah, Saudi Arabia.
authors.forename: Mustafa
authors.initials: M
authors.lastname: Nawajha
authors.affiliation: King Abdullah International Medical Research Center, King Saud bin Abdulaziz University for Health Sciences, Pathology King Abdulaziz Medical City, Jeddah, Saudi Arabia.
authors.forename: Ali
authors.initials: A
authors.lastname: Bawazeer
authors.affiliation: King Abdullah International Medical Research Center, King Saud bin Abdulaziz University for Health Sciences, Pathology King Abdulaziz Medical City, Jeddah, Saudi Arabia.
authors.forename: Ziad
authors.initials: Z
authors.lastname: Baarmah
authors.affiliation: King Faisal Specialist Hospital and Research Center, Jeddah, Saudi Arabia.
authors.forename: Ahmed
authors.initials: A
authors.lastname: Shanaa
authors.affiliation: King Abdullah International Medical Research Center, King Saud bin Abdulaziz University for Health Sciences, Pathology King Abdulaziz Medical City, Jeddah, Saudi Arabia.
authors.forename: Ibrahim
authors.initials: I
authors.lastname: Kadam
authors.affiliation: King Abdullah International Medical Research Center, King Saud bin Abdulaziz University for Health Sciences, Pathology King Abdulaziz Medical City, Jeddah, Saudi Arabia.
authors.forename: Mohamed
authors.initials: M
authors.lastname: Abdelaal
mesh_terms: D000328:Adult; D001800:Blood Specimen Collection / Q000295:instrumentation* / Q000379:methods*; D002112:Calcifediol / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005260:Female; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D008297:Male
chemical_list: D002112:Calcifediol
keywords: 25OHD3; Becton Dickinson; SSTs; plain tubes; vitamin D