title: Comparative study of lanostane-type triterpene acids in different parts of Poria cocos (Schw.) Wolf by UHPLC-Fourier transform MS and UHPLC-triple quadruple MS.
abstract: Poria cocos (Schw.) Wolf is widely used as a traditional Chinese medicine, and approximately 10% of traditional Chinese medicinal preparations contain this material, according to the Chinese Pharmacopoeia (2010 edition). Although the epidermis (fulingpi in Chinese, or FLP) and the inner parts (baifuling in Chinese, or BFL) of P. cocos had different therapeutic applications in history, studies pertaining to a comparative analysis of their chemical constituents have been scarce. UHPLC-DAD-FT/MS(n) has been used in the current study to identify the triterpene acids present in fungus based on a detailed analysis of the fragmentation behavior of 13 standard compounds. This analysis allowed for the identification of 27 triterpene acids, including five groups of isomers and four potential new compounds. Furthermore, a UHPLC-MS/MS method has been developed for quantifying the amounts of nine bioactive triterpene acids in samples of the FLP and BFL, including three 3,4-seco-lanostane-type triterpene acids. These results revealed significant differences in the amounts of these compounds in the FLP and BFL samples. Principal component analysis and partial least squares discriminant analysis of the results for the FLP and BFL samples clearly demonstrated that dehydrotumulosic acid, trametenolic acid, dehydrotrametenolic acid and poricoic acid A were the main compounds contributing to the clusters in FLP and BFL. The observed differences in the chemical compositions of FLP and BFL could provide some explanation of the differences in their clinic applications. This study represents the first reported comprehensive analysis of lanostane-type triterpene acids in FLP and BFL parts of P. cocos.
authors.affiliation: Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, PR China.
authors.forename: Weihao
authors.initials: W
authors.lastname: Wang
authors.affiliation: Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, PR China.
authors.forename: Hongjing
authors.initials: H
authors.lastname: Dong
authors.affiliation: Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, PR China.
authors.forename: Renyi
authors.initials: R
authors.lastname: Yan
authors.affiliation: Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, PR China.
authors.forename: Hua
authors.initials: H
authors.lastname: Li
authors.affiliation: Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, PR China.
authors.forename: Pengyue
authors.initials: P
authors.lastname: Li
authors.affiliation: Wuhan Polythechnic University, Wuhan 430023, PR China.
authors.forename: Ping
authors.initials: P
authors.lastname: Chen
authors.affiliation: Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, PR China. Electronic address: ybinmm@126.com.
authors.forename: Bin
authors.initials: B
authors.lastname: Yang
authors.affiliation: Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, PR China.
authors.forename: Zhimin
authors.initials: Z
authors.lastname: Wang
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005583:Fourier Analysis; D016018:Least-Squares Analysis; D013058:Mass Spectrometry / Q000379:methods*; D055441:Poria / Q000737:chemistry*; D025341:Principal Component Analysis; D012015:Reference Standards; D014315:Triterpenes / Q000032:analysis*
chemical_list: D014315:Triterpenes
keywords: Epidermis; Inner part; Lanostane-type triterpene acid; Poria cocos (Schw.) Wolf; UHPLC–MS(n)