title: [Application of methylation-specific multiplex ligation-dependent probe amplification for the study of DNA methylation in placenta tissues].
abstract: OBJECTIVE
  To study the feasibility of using methylation-specific multiplex ligation-dependent probe amplification (MS-MLPA) for the detection of DNA methylation in placenta tissue.
  
  
  METHODS
  For blood cells from 13 non-pregnant women and 9 euploid placenta, the ratios of DNA methylation were evaluated for 4 genes including CGI149, CGI113, HLCS and ACTB with MS-MLPA and bisulfite sequencing, respectively.
  
  
  RESULTS
  The methylation ratio of the ACTB gene was 0-0.1 for the blood cells when the digestion control was completely digested. The cutoff value for the methylation ratio of MS-MLPA has been determined as 0.1. For the 9 placenta samples, results of MS-MLPA and bisulfite sequencing were concordant for all of the four genes.
  
  
  CONCLUSIONS
  MS-MLPA is an effective alternative to bisulfite sequencing for the assessment of methylation ratios in placental tissues.
authors.affiliation: Department of Obstetrics and Gynecology, the Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, Guangdong 510630, P.R.China. yzzst2011@163.com.
authors.forename: Yuzhu
authors.initials: Y
authors.lastname: Yin
authors.forename: Jun
authors.initials: J
authors.lastname: Zhang
authors.forename: Qin
authors.initials: Q
authors.lastname: She
authors.forename: Qi
authors.initials: Q
authors.lastname: Tian
authors.forename: Junwei
authors.initials: J
authors.lastname: Lin
mesh_terms: D000199:Actins / Q000235:genetics; D000328:Adult; D019731:Carbon-Nitrogen Ligases / Q000235:genetics; D018899:CpG Islands / Q000235:genetics*; D019175:DNA Methylation; D056827:Endosomal Sorting Complexes Required for Transport / Q000235:genetics; D005240:Feasibility Studies; D005260:Female; D006801:Humans; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods*; D010920:Placenta / Q000378:metabolism*; D011247:Pregnancy; D015203:Reproducibility of Results; D012269:Ribosomal Proteins / Q000235:genetics; D055815:Young Adult
chemical_list: D000199:Actins; C498543:CHMP3 protein, human; D056827:Endosomal Sorting Complexes Required for Transport; D012269:Ribosomal Proteins; C027624:ribosomal protein L11; D019731:Carbon-Nitrogen Ligases; C023312:holocarboxylase synthetases