title: Amorphous calcium phosphate nanospheres/polylactide composite coated tantalum scaffold: facile preparation, fast biomineralization and subchondral bone defect repair application.
abstract: Calcium phosphate (CaP) materials are widely used in various biomedical areas such as drug/gene delivery and bone repair/tissue engineering. In this study, amorphous CaP nanospheres synthesized by a simple co-precipitation method are used to prepare the CaP-polylactide (CaP-PLA) composite. Then, the as-prepared CaP-PLA composite is used to coat tantalum (Ta) plates and porous scaffolds. Compared with bare Ta plate, CaP-PLA coated Ta plates show a high performance of surface biomineralization in simulated body fluid (SBF). In addition, the hydrophilicity of the CaP-PLA coated Ta plates is significantly improved. CaP-PLA coated Ta plates with bovine serum albumin (BSA) are prepared and used for the investigation of BSA release in vitro. The experimental results indicate a sustained BSA release property and simultaneous biomineralization of the as-prepared BSA-containing CaP-PLA coated Ta plates. Furthermore, CaP-PLA coated Ta scaffolds are favorable for the human osteoblast-like MG63 cells adhesion and spreading. The vascular endothelial growth factor (VEGF) and transforming growth factor (TGF)-containing CaP-PLA coated porous Ta scaffolds are used for the study of rabbit subchondral bone defect repair, covering with autogeneic periosteums. The as-prepared CaP-PLA composite coated Ta scaffolds are useful to guide the bone regeneration in vivo.
authors.affiliation: Orthopedics Department, Changzheng Hospital, Second Military Medical University, Shanghai 200003, PR China.
authors.forename: Rong
authors.initials: R
authors.lastname: Zhou
authors.affiliation: Orthopedics Department, Changzheng Hospital, Second Military Medical University, Shanghai 200003, PR China.
authors.forename: Wei
authors.initials: W
authors.lastname: Xu
authors.affiliation: State Key Laboratory of High Performance Ceramics and Superfine Microstructure, Shanghai Institute of Ceramics, Chinese Academy of Sciences, Shanghai 200050, PR China. Electronic address: fchen@mail.sic.ac.cn.
authors.forename: Feng
authors.initials: F
authors.lastname: Chen
authors.affiliation: State Key Laboratory of High Performance Ceramics and Superfine Microstructure, Shanghai Institute of Ceramics, Chinese Academy of Sciences, Shanghai 200050, PR China.
authors.forename: Chao
authors.initials: C
authors.lastname: Qi
authors.affiliation: State Key Laboratory of High Performance Ceramics and Superfine Microstructure, Shanghai Institute of Ceramics, Chinese Academy of Sciences, Shanghai 200050, PR China.
authors.forename: Bing-Qiang
authors.initials: BQ
authors.lastname: Lu
authors.affiliation: Orthopedics Department, Changzheng Hospital, Second Military Medical University, Shanghai 200003, PR China.
authors.forename: Hao
authors.initials: H
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of High Performance Ceramics and Superfine Microstructure, Shanghai Institute of Ceramics, Chinese Academy of Sciences, Shanghai 200050, PR China.
authors.forename: Jin
authors.initials: J
authors.lastname: Wu
authors.affiliation: Orthopedics Department, Changzheng Hospital, Second Military Medical University, Shanghai 200003, PR China. Electronic address: qianqr@163.com.
authors.forename: Qi-Rong
authors.initials: QR
authors.lastname: Qian
authors.affiliation: State Key Laboratory of High Performance Ceramics and Superfine Microstructure, Shanghai Institute of Ceramics, Chinese Academy of Sciences, Shanghai 200050, PR China. Electronic address: y.j.zhu@mail.sic.ac.cn.
authors.forename: Ying-Jie
authors.initials: YJ
authors.lastname: Zhu
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000009:adverse effects / Q000737:chemistry; D002130:Calcium Phosphates / Q000737:chemistry*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D006801:Humans; D053761:Nanocomposites / Q000737:chemistry; D054118:Nanospheres / Q000737:chemistry*; D011091:Polyesters / Q000737:chemistry*; D011817:Rabbits; D013635:Tantalum / Q000737:chemistry*; D054457:Tissue Scaffolds / Q000009:adverse effects / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials; D002130:Calcium Phosphates; D011091:Polyesters; C519480:amorphous calcium phosphate; C033616:poly(lactide); D013635:Tantalum
keywords: Biomineralization; Bone defect repair; Calcium phosphate; Drug release; Surface modification; Tantalum scaffold