title: [Evaluation of real-time multiplex PCR effectiveness for group a rotavirus genotyping].
abstract: OBJECTIVE
  Evaluate resolution and diagnostic significance of real-time multiplex PCR (MP RT-PCR) as a platform for group A rotavirus G/P genotyping test-systems.
  
  
  METHODS
  Primer and DNA probe construction for an experimental test-system based on MP RT-PCR was carried out by using specialized PC programs and sequence databases GenBank NCBI, EMBL Nucleotide Sequence Database etc. The experimental genotyping test-system was tested using 116 clinical samples with confirmed rotavirus infection and 14 biosamples negative for group A rotavirus RNA. Selective sequencing of VP7, VP6, VP4 gene mark-erloci was carried out as a reference method forverifying determination of rotavirus genotype.
  
  
  RESULTS
  Specific interaction between primers and DNA probes with genotype-specific loci of retrovirus genome segments and a lack of false-negative signals, complete match ofgenotyping results obtained by MR RT-PCR and sequenc- ing methods were established.
  
  
  CONCLUSIONS
  The resolution of MP RT-PCR methods allows designing test-systems that can confidently identify rotavirus genotypes with effectiveness of 90% and above.
authors.forename: G N
authors.initials: GN
authors.lastname: Bakhtoĭarov
authors.forename: I S
authors.initials: IS
authors.lastname: Kiselev
authors.forename: V V
authors.initials: VV
authors.lastname: Zverev
authors.forename: E B
authors.initials: EB
authors.lastname: Faĭzuloev
mesh_terms: D036022:Capsid Proteins / Q000235:genetics / Q000302:isolation & purification; D003967:Diarrhea / Q000235:genetics / Q000821:virology; D005243:Feces / Q000821:virology; D005838:Genotype; D006801:Humans; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods; D010802:Phylogeny; D012401:Rotavirus / Q000235:genetics* / Q000302:isolation & purification; D012400:Rotavirus Infections / Q000235:genetics* / Q000821:virology; D012703:Serotyping
chemical_list: D036022:Capsid Proteins