title: Facile preparation of organic-inorganic hybrid polymeric ionic liquid monolithic column with a one-pot process for protein separation in capillary electrochromatography.
abstract: An organic-inorganic hybrid monolithic column based on 1-vinyl-3-dodecylimidazolium bromide (VC12Im(+)Br(-)) has been prepared in a single step by combining radical copolymerization with a non-hydrolytic sol-gel (NHSG) process. The NHSG process was significantly shortened to 6 h by using formic acid as catalyst. For comparison, we also prepared polymeric ionic liquid (PIL) monolithic columns by hydrolytic sol-gel and organic polymeric process, respectively. The resulting monolithic columns were characterized by Fourier transform infrared spectra, scanning electron microscopy, and Brunauer-Emmett-Teller. Under the capillary electrochromatography mode, these columns were applied to separate alkylbenzenes, anilines, and proteins, respectively. The results indicated that the NHSG-based hybrid PIL monolithic column exhibited the highest column efficiency among the three types of columns; organic solvent, commonly required by the traditional columns to achieve satisfactory separation efficiency for proteins, was absent in the NHSG-based hybrid PIL monolithic column because of the biocompatibility of the VC12Im(+)Br(-), which was beneficial to analysis of protein containing samples. In order to demonstrate its application potential, the developed NHSG-based hybrid PIL monolithic column was also employed to separate egg white sample.
authors.affiliation: Key Laboratory of Food Nutrition and Safety, Ministry of Education, Tianjin Key Laboratory of Food Nutrition and Safety, Tianjin University of Science and Technology, Tianjin, 300457, China.
authors.forename: Cuicui
authors.initials: C
authors.lastname: Liu
authors.forename: Qiliang
authors.initials: Q
authors.lastname: Deng
authors.forename: Guozhen
authors.initials: G
authors.lastname: Fang
authors.forename: Xue
authors.initials: X
authors.lastname: Feng
authors.forename: Hailong
authors.initials: H
authors.lastname: Qian
authors.forename: Shuo
authors.initials: S
authors.lastname: Wang
mesh_terms: D000818:Animals; D053801:Capillary Electrochromatography / Q000379:methods*; D002645:Chickens; D004529:Egg White / Q000737:chemistry*; D007287:Inorganic Chemicals / Q000737:chemistry*; D052578:Ionic Liquids; D008855:Microscopy, Electron, Scanning; D009930:Organic Chemicals / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry / Q000302:isolation & purification*; D012822:Silicon Dioxide / Q000737:chemistry
chemical_list: D007287:Inorganic Chemicals; D052578:Ionic Liquids; D009930:Organic Chemicals; D011108:Polymers; D011506:Proteins; D012822:Silicon Dioxide