title: Gene expression analysis in early embryos through reverse transcription quantitative PCR (RT-qPCR).
abstract: Real-time, reverse transcription quantitative PCR (RT-qPCR) is a highly sensitive and reproducible technology for the analysis of gene expression patterns. Its ability to detect minute quantities of nucleic acid from multifarious sources makes it an ideal technique for embryonic transcript quantification. However, complex cellular diversity and active transcriptome dynamics in early embryos necessitate particular caution to avoid erroneous results. This chapter is intended to outline basic methodology to design and execute RT-qPCR experiments in pre-implantation embryos.
authors.affiliation: UMR1198 Biologie du Développement et Reproduction, INRA, Jouy-en-Josas, 78350, France.
authors.forename: Nathalie
authors.initials: N
authors.lastname: Peynot
authors.forename: Véronique
authors.initials: V
authors.lastname: Duranthon
authors.forename: Daulat Raheem
authors.initials: DR
authors.lastname: Khan
mesh_terms: D000818:Animals; D017931:DNA Primers; D020869:Gene Expression Profiling / Q000295:instrumentation / Q000379:methods*; D018507:Gene Expression Regulation, Developmental; D060888:Real-Time Polymerase Chain Reaction / Q000295:instrumentation / Q000379:methods*; D015203:Reproducibility of Results; D048348:Reverse Transcription; D057188:Workflow
chemical_list: D017931:DNA Primers