title: Bacterial bioluminescence response to long-term exposure to reverse osmosis treated effluents from dye industries.
abstract: The bacterial bioluminescence assay is one of the novel means for toxicity detection. The bioluminescence response of 2 marine bioluminescent bacteria was tested upon their long-term exposure to 9 different reverse osmosis (RO) rejects with varying chemical composition sampled from various dye industries. Bioluminescent bacteria were cultured in the RO reject samples, at different concentrations, and their growth rate and luminescence was measured for 24 h. The RO reject samples caused sublethal effects upon exposure and retarded the growth of bacteria, confirming their toxic nature. Further, continuation of the exposure showed that the initial luminescence, though reduced, recovered and increased beyond the control cultures irrespective of cell density, and finally decreased once again. The present study emphasizes the need of evolving a long-term exposure assay and shows that the method followed in this study is suitable to evaluate the toxicants that exert delayed toxicity, using lower concentrations of toxicants as well as coloured samples.
authors.affiliation: a CSIR - National Institute of Oceanography, Dona Paula, Goa 403 004, India.
authors.forename: J
authors.initials: J
authors.lastname: Ravindran
authors.forename: B
authors.initials: B
authors.lastname: Manikandan
authors.forename: P V
authors.initials: PV
authors.lastname: Shirodkar
authors.forename: K X
authors.initials: KX
authors.lastname: Francis
authors.forename: R
authors.initials: R
authors.lastname: Mani Murali
authors.forename: P
authors.initials: P
authors.lastname: Vethamony
mesh_terms: D001419:Bacteria / Q000187:drug effects*; D005374:Filtration; D007220:Industrial Waste; D008163:Luminescent Measurements / Q000592:standards; D009995:Osmosis; D013995:Time; D014874:Water Pollutants, Chemical / Q000378:metabolism / Q000633:toxicity*; D018508:Water Purification / Q000592:standards
chemical_list: D007220:Industrial Waste; D014874:Water Pollutants, Chemical
keywords: bioluminescence; delayed toxicity; dye industry; effet sublétal; industrie des colorants; rejet d’osmose inverse; reverse osmosis reject; sublethal effect; toxicité retardée