title: Transcriptome Analysis of the Small Brown Planthopper, Laodelphax striatellus Carrying Rice stripe virus.
abstract: Rice stripe virus (RSV), the type member of the genus Tenuivirus, transmits by the feeding behavior of small brown planthopper (SBPH), Laodelphax striatellus. To investigate the interactions between the virus and vector insect, total RNA was extracted from RSV-viruliferous SBPH (RVLS) and non-viruliferous SBPH (NVLS) adults to construct expressed sequence tag databases for comparative transcriptome analysis. Over 30 million bases were sequenced by 454 pyrosequencing to construct 1,538 and 953 of isotigs from the mRNA of RVLS and NVLS, respectively. The gene ontology (GO) analysis demonstrated that both libraries have similar GO structures, however, the gene expression pattern analysis revealed that 17.8% and 16.8% of isotigs were up- and down-regulated significantly in the RVLS, respectively. These RSV-dependently regulated genes possibly have important roles in the physiology of SBPH, transmission of RSV, and RSV and SBPH interaction.
authors.affiliation: Department of Agricultural Biotechnology, College of Agriculture and Life Sciences, Seoul National University, Seoul 151-742, Korea.
authors.forename: Joo Hyun
authors.initials: JH
authors.lastname: Lee
authors.affiliation: Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul 151-742, Korea.
authors.forename: Jae Young
authors.initials: JY
authors.lastname: Choi
authors.affiliation: Department of Agricultural Biotechnology, College of Agriculture and Life Sciences, Seoul National University, Seoul 151-742, Korea.
authors.forename: Xue Ying
authors.initials: XY
authors.lastname: Tao
authors.affiliation: Department of Agricultural Biology, College of Agricultural Life Sciences, Chonbuk National University, Jeonju 561-756, Korea.
authors.forename: Jae Su
authors.initials: JS
authors.lastname: Kim
authors.affiliation: Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul 151-742, Korea.
authors.forename: Woojin
authors.initials: W
authors.lastname: Kim
authors.affiliation: Department of Agricultural Biotechnology, College of Agriculture and Life Sciences, Seoul National University, Seoul 151-742, Korea ; Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul 151-742, Korea.
authors.forename: Yeon Ho
authors.initials: YH
authors.lastname: Je
keywords: Laodelphax striatellus; Rice stripe virus; small brown planthopper; tenuivirus; transcriptome