title: Parapedobacter indicus sp. nov., isolated from hexachlorocyclohexane-contaminated soil.
abstract: Strain RK1(T), a Gram-stain-negative, non-spore-forming, rod-shaped, non-motile bacterium was isolated from a hexachlorocyclohexane (HCH) dumpsite, Lucknow, India. 16S rRNA gene sequence analysis revealed that strain RK1(T) belongs to the family Sphingobacteriaceae and showed highest sequence similarity to Parapedobacter koreensis Jip14(T) (95.63%). The major cellular fatty acids of strain RK1(T) were iso-C15:0, summed feature 3 (C16:1ω7c and/or C16:1ω6c), iso-C17:0 3-OH, summed feature 9 (10-methyl C16:0 and/or iso-C17:1ω9c), iso-C15:0 3-OH and C16 : 0. The major respiratory pigment and polyamine of RK1(T) were menaquinone (MK-7) and homospermidine, respectively. The main polar lipids were phosphatidylethanolamine and sphingolipid. The G+C content of the DNA was 44.5 mol%. The results of physiological and biochemical tests and 16S rRNA sequence analysis clearly demonstrated that strain RK1(T) represents a novel species of the genus Parapedobacter, for which the name Parapedobacter indicus sp. nov. is proposed. The type strain is RK1(T) ( = DSM 28470(T) =MCC 2546(T)).
authors.affiliation: Molecular Biology Laboratory, Department of Zoology, University of Delhi, Delhi-110007, India.
authors.forename: Roshan
authors.initials: R
authors.lastname: Kumar
authors.affiliation: Molecular Biology Laboratory, Department of Zoology, University of Delhi, Delhi-110007, India.
authors.forename: Vatsala
authors.initials: V
authors.lastname: Dwivedi
authors.affiliation: Molecular Biology Laboratory, Department of Zoology, University of Delhi, Delhi-110007, India.
authors.forename: Namita
authors.initials: N
authors.lastname: Nayyar
authors.affiliation: Molecular Biology Laboratory, Department of Zoology, University of Delhi, Delhi-110007, India.
authors.forename: Helianthous
authors.initials: H
authors.lastname: Verma
authors.affiliation: Molecular Biology Laboratory, Department of Zoology, University of Delhi, Delhi-110007, India.
authors.forename: Amit Kumar
authors.initials: AK
authors.lastname: Singh
authors.affiliation: Molecular Biology Laboratory, Department of Zoology, University of Delhi, Delhi-110007, India.
authors.forename: Pooja
authors.initials: P
authors.lastname: Rani
authors.affiliation: ICAR-Indian Institute of Soil Science, Bhopal 462038, Madhya Pradesh, India.
authors.forename: D L N
authors.initials: DLN
authors.lastname: Rao
authors.affiliation: Molecular Biology Laboratory, Department of Zoology, University of Delhi, Delhi-110007, India.
authors.forename: Rup
authors.initials: R
authors.lastname: Lal
mesh_terms: D015373:Bacterial Typing Techniques; D041963:Bacteroidetes / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D001556:Hexachlorocyclohexane / Q000032:analysis; D007194:India; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000032:analysis; D013095:Spermidine / Q000737:chemistry; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D012989:Soil Pollutants; D024482:Vitamin K 2; D001556:Hexachlorocyclohexane; C062629:menaquinone 7; D013095:Spermidine