title: Influence of racing on the serum concentrations of acute-phase proteins and bone metabolism biomarkers in racing greyhounds.
abstract: This study was designed to evaluate the influence of racing on the serum concentrations of the acute-phase proteins (APPs) C-reactive protein (CRP), haptoglobin (Hp) and serum amyloid A (SAA) in 32 endurance-racing greyhounds. The study also aimed to investigate the effect of a 7 km race on the bone biomarkers osteocalcin (OC), bone-specific alkaline phosphatase (b-ALP) and pyridinoline cross-links (PYD). Total white blood cell (WBC) count, and the serum concentrations of cortisol, tumour necrosis factor-α (TNF-α), vitamin D and testosterone were also determined. Blood samples were collected 24 h prior to (T0) and within 2 h of completion of the race (T1). Compared to baseline values, WBC count did not change significantly (P = 0.2300), serum cortisol, Hp and SAA increased, while TNF-α and CRP decreased (P <0.0001 for each). There were no significant differences between the pre- and post-race serum concentrations of OC and PYD (P = 0.9500 and P = 0.2600, respectively), but serum b-ALP increased significantly (P = 0.0004). Serum concentrations of vitamin D and testosterone increased after racing (P = 0.0100 and P <0.0001, respectively). In this study, a 7 km race stimulated an acute-phase response, demonstrated by significant increases in the serum concentrations Hp and SAA in racing greyhounds. Increased serum b-ALP post-race probably indicates a change in bone metabolism and deserves further study.
authors.affiliation: Department of Veterinary Medicine, College of Agriculture and Veterinary Medicine, Qassim University, Saudi Arabia; Department of Animal Medicine, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Egypt.
authors.forename: M
authors.initials: M
authors.lastname: Tharwat
authors.affiliation: Department of Veterinary Medicine, College of Agriculture and Veterinary Medicine, Qassim University, Saudi Arabia.
authors.forename: F
authors.initials: F
authors.lastname: Al-Sobayil
authors.affiliation: Bovine Ambulatory Clinic, Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Montreal, Saint-Hyacinthe, Canada. Electronic address: s.buczinski@umontreal.ca.
authors.forename: S
authors.initials: S
authors.lastname: Buczinski
mesh_terms: D000209:Acute-Phase Proteins / Q000378:metabolism*; D000818:Animals; D015415:Biomarkers / Q000097:blood / Q000378:metabolism; D001842:Bone and Bones / Q000378:metabolism*; D004285:Dogs / Q000097:blood / Q000502:physiology*; D008297:Male; D010805:Physical Conditioning, Animal; D012420:Running
chemical_list: D000209:Acute-Phase Proteins; D015415:Biomarkers
keywords: Acute-phase proteins; Biomarkers; Bone metabolism; Greyhounds; Racing; Stress