title: One-step immobilization of antibodies for α-1-fetoprotein immunosensor based on dialdehyde cellulose/ionic liquid composite.
abstract: A novel immunosensor for α-1-fetoprotein based on dialdehyde cellulose/ionic liquid composite film as a matrix has been developed. Microcrystalline cellulose was activated by sodium metaperiodate to produce dialdehyde cellulose. Antibodies can be immobilized on the electrode by a one-step method through covalent bonding of the aldehyde groups of dialdehyde cellulose with the amino groups of antibodies, in which no additional chemical cross-linking step is required. Moreover, ionic liquid added can improve the conductivity of the sensing interface and, therefore, can enhance the electrochemical signal. In this work, α-1-fetoprotein was detected within the range from 0.1 to 60ngml(-1) with a detection limit of 0.07ngml(-1) (signal/noise=3). The proposed immunosensor had good specificity and reproducibility. It was used to determine real samples with satisfactory results.
authors.affiliation: College of Chemistry and Chemical Engineering, Hunan University of Arts and Science, Changde, Hunan 415000, China. Electronic address: sgyrab@yahoo.com.cn.
authors.forename: Guangyu
authors.initials: G
authors.lastname: Shen
authors.affiliation: College of Chemistry and Chemical Engineering, Hunan University of Arts and Science, Changde, Hunan 415000, China.
authors.forename: Xiangyang
authors.initials: X
authors.lastname: Zhang
authors.affiliation: College of Chemistry and Chemical Engineering, Hunan University of Arts and Science, Changde, Hunan 415000, China.
authors.forename: Youming
authors.initials: Y
authors.lastname: Shen
authors.affiliation: College of Chemistry and Chemical Engineering, Hunan University of Arts and Science, Changde, Hunan 415000, China.
authors.forename: Songbai
authors.initials: S
authors.lastname: Zhang
authors.affiliation: College of Chemistry and Chemical Engineering, Hunan University of Arts and Science, Changde, Hunan 415000, China.
authors.forename: Li
authors.initials: L
authors.lastname: Fang
mesh_terms: D000818:Animals; D055910:Antibodies, Immobilized / Q000737:chemistry* / Q000276:immunology; D015374:Biosensing Techniques / Q000379:methods*; D002482:Cellulose / Q000031:analogs & derivatives* / Q000737:chemistry; D004563:Electrochemistry; D004566:Electrodes; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D052578:Ionic Liquids / Q000737:chemistry*; D015203:Reproducibility of Results; D000509:alpha-Fetoproteins / Q000032:analysis* / Q000276:immunology
chemical_list: D055910:Antibodies, Immobilized; D052578:Ionic Liquids; D000509:alpha-Fetoproteins; D002482:Cellulose; C058684:2,3-dialdehydocellulose
keywords: Dialdehyde cellulose; Electrochemical immunosensor; Ionic liquid; One-step immobilization of antibody; α-1-Fetoprotein