title: Flexible and accessible workflows for improved proteogenomic analysis using the Galaxy framework.
abstract: Proteogenomics combines large-scale genomic and transcriptomic data with mass-spectrometry-based proteomic data to discover novel protein sequence variants and improve genome annotation. In contrast with conventional proteomic applications, proteogenomic analysis requires a number of additional data processing steps. Ideally, these required steps would be integrated and automated via a single software platform offering accessibility for wet-bench researchers as well as flexibility for user-specific customization and integration of new software tools as they emerge. Toward this end, we have extended the Galaxy bioinformatics framework to facilitate proteogenomic analysis. Using analysis of whole human saliva as an example, we demonstrate Galaxy's flexibility through the creation of a modular workflow incorporating both established and customized software tools that improve depth and quality of proteogenomic results. Our customized Galaxy-based software includes automated, batch-mode BLASTP searching and a Peptide Sequence Match Evaluator tool, both useful for evaluating the veracity of putative novel peptide identifications. Our complex workflow (approximately 140 steps) can be easily shared using built-in Galaxy functions, enabling their use and customization by others. Our results provide a blueprint for the establishment of the Galaxy framework as an ideal solution for the emerging field of proteogenomics.
authors.affiliation: Center for Mass Spectrometry and Proteomics, University of Minnesota , 43 Gortner Laboratory, 1479 Gortner Avenue, St. Paul, Minnesota 55108, United States.
authors.forename: Pratik D
authors.initials: PD
authors.lastname: Jagtap
authors.forename: James E
authors.initials: JE
authors.lastname: Johnson
authors.forename: Getiria
authors.initials: G
authors.lastname: Onsongo
authors.forename: Fredrik W
authors.initials: FW
authors.lastname: Sadler
authors.forename: Kevin
authors.initials: K
authors.lastname: Murray
authors.forename: Yuanbo
authors.initials: Y
authors.lastname: Wang
authors.forename: Gloria M
authors.initials: GM
authors.lastname: Shenykman
authors.forename: Sricharan
authors.initials: S
authors.lastname: Bandhakavi
authors.forename: Lloyd M
authors.initials: LM
authors.lastname: Smith
authors.forename: Timothy J
authors.initials: TJ
authors.lastname: Griffin
mesh_terms: D018076:DNA, Complementary / Q000235:genetics; D015723:Gene Library; D006801:Humans; D020543:Proteome / Q000235:genetics / Q000378:metabolism*; D040901:Proteomics; D016364:Reading Frames; D012463:Saliva / Q000378:metabolism*; D012984:Software
chemical_list: D018076:DNA, Complementary; D020543:Proteome
keywords: customized database generation; peptide corresponding to a novel proteoform; peptide-spectral match evaluation; proteogenomics; salivary proteins; workflows