title: Polymorphisms +45T>G and +276G>T of the adiponectin gene does not affect plasma adiponectin level and carotid intima-media thickness in patients with diabetes mellitus type 2.
abstract: OBJECTIVE
  Despite increasing evidence of adiponectin's anti-inflammatory and antiatherogenic effects, its role in atherogenesis remains uncertain. The aim of the present study is to investigate the association between +45T>G and +276G>T polymorphisms of the adiponectin gene and both plasma adiponectin levels and carotid intima-media thickness in patients with diabetes mellitus type 2.
  
  
  METHODS
  301 diabetic patients, divided into three categories on the basis on BMI were enrolled in the study. Carotid intima-media thickness (CIMT) was assessed ultrasonographically. Plasma adiponectin levels were measured by enzyme-linked immunosorbent assay (ELISA). Genotypes were determined by real-time PCR.
  
  
  RESULTS
  Adiponectin level and prevalence of the G allele of 45T>G polymorphism decreased significantly with increasing BMI category. G allele of +45T>G polymorphism was associated with higher plasma adiponectin level only after adjustment for age, sex and BMI. No statistically significant difference in CIMT and +276T>G genotypes distribution was observed between BMI categories. None of the polymorphisms as well as plasma adiponectin level was associated with CIMT after adjustment for covariates.
  
  
  CONCLUSIONS
  The G allele of the +45T>G polymorphism is not independently associated with plasma adiponectin level and is not associated with CIMT. +276G>T polymorphism is not associated with plasma adiponectin levels and CIMT in diabetic patients.
authors.affiliation: Institute of Histology and Embryology, Medical Faculty, University of Ljubljana, Ljubljana, Slovenia - daniel.petrovic@mf.uni-lj.si.
authors.forename: J
authors.initials: J
authors.lastname: Nikolajević-Starčević
authors.forename: A
authors.initials: A
authors.lastname: Pleskovič
authors.forename: M
authors.initials: M
authors.lastname: Santl Letonja
authors.forename: Z
authors.initials: Z
authors.lastname: Jenko Pražnikar
authors.forename: D
authors.initials: D
authors.lastname: Petrovič
mesh_terms: D052242:Adiponectin / Q000097:blood / Q000235:genetics*; D000368:Aged; D015415:Biomarkers / Q000097:blood; D015992:Body Mass Index; D002340:Carotid Artery Diseases / Q000097:blood / Q000175:diagnosis / Q000235:genetics*; D059168:Carotid Intima-Media Thickness; D003430:Cross-Sectional Studies; D003924:Diabetes Mellitus, Type 2 / Q000097:blood / Q000150:complications / Q000175:diagnosis / Q000235:genetics*; D003925:Diabetic Angiopathies / Q000097:blood / Q000175:diagnosis / Q000235:genetics*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D006801:Humans; D008297:Male; D008875:Middle Aged; D010641:Phenotype; D011110:Polymorphism, Genetic; D011237:Predictive Value of Tests; D060888:Real-Time Polymerase Chain Reaction; D012307:Risk Factors; D018615:Ultrasonography, Doppler, Color; D018617:Ultrasonography, Doppler, Pulsed
chemical_list: C499918:ADIPOQ protein, human; D052242:Adiponectin; D015415:Biomarkers