title: Lumican: a new inhibitor of matrix metalloproteinase-14 activity.
abstract: We previously showed that lumican regulates MMP-14 expression. The aim of this study was to compare the effect of lumican and decorin on MMP-14 activity. In contrast to decorin, the glycosylated form of lumican was able to significantly decrease MMP-14 activity in B16F1 melanoma cells. Our results suggest that a direct interaction occurs between lumican and MMP-14. Lumican behaves as a competitive inhibitor which leads to a complete blocking of the activity of MMP-14. It binds to the catalytic domain of MMP-14 with moderate affinity (KD∼275 nM). Lumican may protect collagen against MMP-14 proteolysis, thus influencing cell-matrix interaction in tumor progression.
authors.affiliation: CNRS UMR 7369, Matrice Extracellulaire et Dynamique Cellulaire, Reims, France; Université de Reims Champagne Ardenne, Laboratoire de Biochimie Médicale et de Biologie Moléculaire, Reims, France.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Pietraszek
authors.affiliation: CNRS UMR 7369, Matrice Extracellulaire et Dynamique Cellulaire, Reims, France; Université de Reims Champagne Ardenne, Laboratoire de Biochimie Médicale et de Biologie Moléculaire, Reims, France.
authors.forename: Aurore
authors.initials: A
authors.lastname: Chatron-Colliet
authors.affiliation: CNRS UMR 7369, Matrice Extracellulaire et Dynamique Cellulaire, Reims, France; Université de Reims Champagne Ardenne, Laboratoire de Biochimie Médicale et de Biologie Moléculaire, Reims, France.
authors.forename: Stéphane
authors.initials: S
authors.lastname: Brézillon
authors.affiliation: CNRS UMR 7369, Matrice Extracellulaire et Dynamique Cellulaire, Reims, France; Université de Reims Champagne Ardenne, Laboratoire de Biochimie Médicale et de Biologie Moléculaire, Reims, France.
authors.forename: Corinne
authors.initials: C
authors.lastname: Perreau
authors.affiliation: Department of Immunochemistry, Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wrocław, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Jakubiak-Augustyn
authors.affiliation: Department of Immunochemistry, Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wrocław, Poland.
authors.forename: Hubert
authors.initials: H
authors.lastname: Krotkiewski
authors.affiliation: CNRS UMR 7369, Matrice Extracellulaire et Dynamique Cellulaire, Reims, France; Université de Reims Champagne Ardenne, Laboratoire de Biochimie Médicale et de Biologie Moléculaire, Reims, France; CHU de Reims, Laboratoire Central de Biochimie, Reims, France.
authors.forename: François-Xavier
authors.initials: FX
authors.lastname: Maquart
authors.affiliation: CNRS UMR 7369, Matrice Extracellulaire et Dynamique Cellulaire, Reims, France; Université de Reims Champagne Ardenne, Laboratoire de Biochimie Médicale et de Biologie Moléculaire, Reims, France. Electronic address: yanusz.wegrowski@univ-reims.fr.
authors.forename: Yanusz
authors.initials: Y
authors.lastname: Wegrowski
mesh_terms: D000818:Animals; D001667:Binding, Competitive; D045744:Cell Line, Tumor; D011508:Chondroitin Sulfate Proteoglycans / Q000378:metabolism / Q000494:pharmacology*; D003094:Collagen / Q000378:metabolism; D006801:Humans; D007632:Keratan Sulfate / Q000378:metabolism / Q000494:pharmacology*; D000071234:Lumican; D053511:Matrix Metalloproteinase 14 / Q000378:metabolism*; D061965:Matrix Metalloproteinase Inhibitors / Q000378:metabolism / Q000494:pharmacology*; D051379:Mice; D059748:Proteolysis / Q000187:drug effects
chemical_list: D011508:Chondroitin Sulfate Proteoglycans; C000604306:LUM protein, human; C000604307:Lum protein, mouse; D000071234:Lumican; D061965:Matrix Metalloproteinase Inhibitors; D003094:Collagen; D007632:Keratan Sulfate; C507086:MMP14 protein, human; D053511:Matrix Metalloproteinase 14
keywords: Decorin; Lumican; MMP-14; Small leucine-rich proteoglycan