title: Lipozyme RM IM-catalyzed acidolysis of Cinnamomum camphora seed oil with oleic acid to produce human milk fat substitutes enriched in medium-chain fatty acids.
abstract: In the present study, a human milk fat substitute (HMFS) enriched in medium-chain fatty acids (MCFAs) was synthesized through acidolysis reaction from Cinnamomum camphora seed oil (CCSO) with oleic acid in a solvent-free system. A commercial immobilized lipase, Lipozyme RM IM, from Rhizomucor miehei, was facilitated as a biocatalyst. Effects of different reaction conditions, including substrate molar ratio, enzyme concentration, reaction temperature, and reaction time were investigated using response surface methodology (RSM) to obtain the optimal oleic acid incorporation. After optimization, results showed that the maximal incorporation of oleic acid into HMFS was 59.68%. Compared with CCSO, medium-chain fatty acids at the sn-2 position of HMFS accounted for >70%, whereas oleic acid was occupied predominantly at the sn-1,3 position (78.69%). Meanwhile, triacylglycerol (TAG) components of OCO (23.93%), CCO (14.94%), LaCO (13.58%), OLaO (12.66%), and OOO (11.13%) were determined as the major TAG species in HMFS. The final optimal reaction conditions were carried out as follows: substrate molar ratio (oleic acid/CCSO), 5:1; enzyme concentration, 12.5% (w/w total reactants); reaction temperature, 60 °C; and reaction time, 28 h. The reusability of Lipozyme RM IM in the acidolysis reaction was also evaluated, and it was found that it could be reused up to 9 times without significant loss of activities. Urea inclusion method was used to separate and purify the synthetic product. As the ratio of HMFS/urea increased to 1:2, the acid value lowered to the minimum. In a scale-up experiment, the contents of TAG and total tocopherols in HMFS (modified CCSO) were 77.28% and 12.27 mg/100 g, respectively. All of the physicochemical indices of purified product were within food standards. Therefore, such a MCFA-enriched HMFS produced by using the acidolysis method might have potential application in the infant formula industry.
authors.affiliation: State Key Laboratory of Food Science and Technology, Institute for Advanced Study and ‡College of Food Science and Technology, Nanchang University , Nanchang, Jiangxi 330047, China.
authors.forename: Xian-Guo
authors.initials: XG
authors.lastname: Zou
authors.forename: Jiang-Ning
authors.initials: JN
authors.lastname: Hu
authors.forename: Man-Li
authors.initials: ML
authors.lastname: Zhao
authors.forename: Xue-Mei
authors.initials: XM
authors.lastname: Zhu
authors.forename: Hong-Yan
authors.initials: HY
authors.lastname: Li
authors.forename: Xiao-Ru
authors.initials: XR
authors.lastname: Liu
authors.forename: Rong
authors.initials: R
authors.lastname: Liu
authors.forename: Ze-Yuan
authors.initials: ZY
authors.lastname: Deng
mesh_terms: D055162:Biocatalysis; D027423:Cinnamomum camphora / Q000737:chemistry*; D019358:Fat Substitutes / Q000737:chemistry*; D005227:Fatty Acids / Q000032:analysis*; D005656:Fungal Proteins / Q000737:chemistry; D006801:Humans; D041943:Infant Formula / Q000737:chemistry; D008049:Lipase / Q000737:chemistry*; D008895:Milk, Human / Q000737:chemistry; D019301:Oleic Acid / Q000737:chemistry*; D010938:Plant Oils / Q000737:chemistry*; D020103:Rhizomucor / Q000201:enzymology; D012639:Seeds / Q000737:chemistry*
chemical_list: D019358:Fat Substitutes; D005227:Fatty Acids; D005656:Fungal Proteins; D010938:Plant Oils; D019301:Oleic Acid; C093666:Lipozyme; D008049:Lipase
keywords: Cinnamomum camphora seed oil (CCSO); Lipozyme RM IM; human milk fat substitute (HMFS); response surface methodology (RSM)