title: Biomimetic apatite-coated porous PVA scaffolds promote the growth of breast cancer cells.
abstract: Recapitulating the native environment of bone tissue is essential to develop in vitro models of breast cancer bone metastasis. The bone is a composite material consisting of organic matrix and inorganic mineral phase, primarily hydroxyapatite. In this study, we report the mineralization of porous poly vinyl alcohol (PVA) scaffolds upon incubation in modified Hanks' Balanced Salt Solution (HBSS) for 14 days. Scanning electron microscopy, energy dispersive X-ray analysis, and X-ray diffraction analysis revealed that the deposited minerals have composition similar to hydroxyapatite. The study demonstrated that the rate of nucleation and growth of minerals was faster on surfaces of less porous scaffolds. However, upon prolonged incubation, formation of mineral layer was observed on the surface of all the scaffolds. In addition, the study also demonstrated that 3D mineralization only occurred for scaffolds with highly interconnected porous networks. The mineralization of the scaffolds promoted the adsorption of serum proteins and consequently, the adhesion and proliferation of breast cancer cells.
authors.affiliation: Department of Mechanical Engineering, University of Michigan, Dearborn, 4901 Evergreen Road, Dearborn, MI 48128, United States.
authors.forename: Mao
authors.initials: M
authors.lastname: Ye
authors.affiliation: Department of Mechanical Engineering, University of Michigan, Dearborn, 4901 Evergreen Road, Dearborn, MI 48128, United States.
authors.forename: Pravansu
authors.initials: P
authors.lastname: Mohanty
authors.affiliation: Department of Mechanical Engineering, University of Michigan, Dearborn, 4901 Evergreen Road, Dearborn, MI 48128, United States. Electronic address: gargi@umich.edu.
authors.forename: Gargi
authors.initials: G
authors.lastname: Ghosh
mesh_terms: D040761:Biomimetic Materials / Q000737:chemistry*; D001943:Breast Neoplasms; D002448:Cell Adhesion / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D017886:Durapatite / Q000737:chemistry*; D005260:Female; D005612:Freeze Drying; D006801:Humans; D008855:Microscopy, Electron, Scanning; D011143:Polyvinyl Chloride / Q000737:chemistry*; D016062:Porosity; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D011143:Polyvinyl Chloride; D017886:Durapatite
keywords: Breast cancer cells; HBSS; Interconnected pores; Mineralization; PVA scaffold