title: Hawaiinolides E-G, cytotoxic cassane and cleistanthane diterpenoids from the entomogenous fungus Paraconiothyrium hawaiiense.
abstract: Hawaiinolides E-G (1-3), three additional new secondary metabolites including two cassane (1 and 2) types of diterpene lactones and one cleistanthane (3) diterpenoid, were isolated from the scale-up fermentation extract of Paraconiothyrium hawaiiense, an entomogenous fungus isolated from the Septobasidium-infected insect Diaspidiotus sp. The structures of 1-3 were elucidated by nuclear magnetic resonance experiments, and 1 and 3 were further confirmed by X-ray crystallography. The absolute configurations of 1 and 3 were assigned by single-crystal X-ray diffraction analysis using Cu Kα radiation, whereas that of 2 was deduced via the circular dichroism data. Compound 1 showed significant cytotoxic effects against the human tumor cell line T24, with an IC₅₀ value (9.32 μM) comparable to that of the positive control cisplatin.
authors.affiliation: State Key Laboratory of Toxicology & Medical Countermeasures, Beijing Institute of Pharmacology & Toxicology, Beijing 100850, PR China; Institute of Microbiology, Chinese Academy of Sciences, Beijing 100190, PR China.
authors.forename: Shenxi
authors.initials: S
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Toxicology & Medical Countermeasures, Beijing Institute of Pharmacology & Toxicology, Beijing 100850, PR China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Toxicology & Medical Countermeasures, Beijing Institute of Pharmacology & Toxicology, Beijing 100850, PR China.
authors.forename: Chen
authors.initials: C
authors.lastname: Zhao
authors.affiliation: State Key Laboratory of Toxicology & Medical Countermeasures, Beijing Institute of Pharmacology & Toxicology, Beijing 100850, PR China.
authors.forename: Fengxia
authors.initials: F
authors.lastname: Ren
authors.affiliation: Institute of Microbiology, Chinese Academy of Sciences, Beijing 100190, PR China.
authors.forename: Xingzhong
authors.initials: X
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Toxicology & Medical Countermeasures, Beijing Institute of Pharmacology & Toxicology, Beijing 100850, PR China. Electronic address: cheys@im.ac.cn.
authors.forename: Yongsheng
authors.initials: Y
authors.lastname: Che
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry* / Q000302:isolation & purification; D001203:Ascomycota / Q000737:chemistry*; D045744:Cell Line, Tumor; D004224:Diterpenes / Q000737:chemistry* / Q000302:isolation & purification; D004354:Drug Screening Assays, Antitumor; D006430:Hemiptera / Q000382:microbiology; D006801:Humans; D020128:Inhibitory Concentration 50; D015394:Molecular Structure
chemical_list: D000970:Antineoplastic Agents; D004224:Diterpenes; C000597669:hawaiinolide E; C000597670:hawaiinolide F; C000597671:hawaiinolide G
keywords: Cytotoxicity; Diterpenoid; Entomogenous fungus; Paraconiothyrium hawaiiense; Sonomolide B (PubChem CID: 465971)