title: Multiple paramagnetic effects through a tagged reporter protein.
abstract: Paramagnetic effects provide unique information about the structure and dynamics of biomolecules. We developed a method in which the lanthanoid tag is not directly attached to the protein of interest, but instead to a "reporter" protein, which binds and then transmits paramagnetic information to the target. The designed method allows access to a large number of paramagnetic restraints and residual dipolar couplings produced from independent molecular alignments in high-molecular-weight proteins with unknown 3D structure.
authors.affiliation: Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Göttingen (Germany); German Center for Neurodegenerative Diseases (DZNE), Göttingen (Germany); Center for the Molecular Physiology of the Brain, University Medical Center, Göttingen (Germany).
authors.forename: Aldo R
authors.initials: AR
authors.lastname: Camacho-Zarco
authors.forename: Francesca
authors.initials: F
authors.lastname: Munari
authors.forename: Melanie
authors.initials: M
authors.lastname: Wegstroth
authors.forename: Wei-Min
authors.initials: WM
authors.lastname: Liu
authors.forename: Marcellus
authors.initials: M
authors.lastname: Ubbink
authors.forename: Stefan
authors.initials: S
authors.lastname: Becker
authors.forename: Markus
authors.initials: M
authors.lastname: Zweckstetter
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000737:chemistry; D000595:Amino Acid Sequence; D004926:Escherichia coli / Q000737:chemistry; D006801:Humans; D028581:Lanthanoid Series Elements / Q000737:chemistry*; D058619:Maltose-Binding Proteins / Q000737:chemistry; D008958:Models, Molecular; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods; D017434:Protein Structure, Tertiary; D011506:Proteins / Q000737:chemistry*; D025801:Ubiquitin / Q000737:chemistry
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D028581:Lanthanoid Series Elements; D058619:Maltose-Binding Proteins; D011506:Proteins; D025801:Ubiquitin
keywords: NMR spectroscopy; lanthanoids; paramagnetism; proteins; structural biology