title: siRNA liposome-gold nanorod vectors for multispectral optoacoustic tomography theranostics.
abstract: Therapeutic applications of gene silencing using siRNA have seen increasing interest over the past decade. The optimization of the delivery and biodistribution of siRNA using liposome-gold nanorod (AuNRs) nanoscale carriers can greatly benefit from adept imaging methods that can visualize the time-resolved delivery performance of such vectors. In this work, we describe the effect of AuNR length incorporated with liposomes and show their complexation with siRNA as a novel gene delivery vehicle. We demonstrate the application of multispectral optoacoustic tomography (MSOT) to longitudinally visualize the localisation of siRNA carrying liposome-AuNR hybrids within tumors. Combination of in vivo MSOT with ex vivo fluorescence cryo-slice imaging offers further insight into the siRNA transport and activity obtained.
authors.affiliation: Chair for Biological Imaging, Technische Universität München, Ismaningerstr. 22, 81675 München, Germany. v.ntziachristos@tum.de.
authors.forename: Adrian
authors.initials: A
authors.lastname: Taruttis
authors.forename: Neus
authors.initials: N
authors.lastname: Lozano
authors.forename: Antonio
authors.initials: A
authors.lastname: Nunes
authors.forename: Dhifaf A
authors.initials: DA
authors.lastname: Jasim
authors.forename: Nicolas
authors.initials: N
authors.lastname: Beziere
authors.forename: Eva
authors.initials: E
authors.lastname: Herzog
authors.forename: Kostas
authors.initials: K
authors.lastname: Kostarelos
authors.forename: Vasilis
authors.initials: V
authors.lastname: Ntziachristos
mesh_terms: D000818:Animals; D004337:Drug Carriers / Q000138:chemical synthesis / Q000737:chemistry; D020868:Gene Silencing; D018014:Gene Transfer Techniques; D005822:Genetic Vectors; D006046:Gold / Q000737:chemistry; D019073:HT29 Cells; D006801:Humans; D008081:Liposomes; D051379:Mice; D043942:Nanotubes / Q000737:chemistry; D034741:RNA, Small Interfering / Q000008:administration & dosage* / Q000737:chemistry / Q000378:metabolism; D000068936:Theranostic Nanomedicine / Q000379:methods*; D014018:Tissue Distribution; D041622:Tomography, Optical / Q000379:methods*; D014407:Tumor Cells, Cultured
chemical_list: D004337:Drug Carriers; D008081:Liposomes; D034741:RNA, Small Interfering; D006046:Gold