title: FT-IR microspectrometry reveals the variation of membrane polarizability due to epigenomic effect on epithelial ovarian cancer.
abstract: Ovarian cancer, as well as other cancers, is primarily caused by methylation at cytosines in CpG islands, but the current marker for ovarian cancer is low in sensitivity and failed in early-stage detection. Fourier transform infrared (FT-IR) spectroscopy is powerful in analysis of functional groups within molecules, and infrared microscopy illustrates the location of specific groups within single cells. In this study, we applied HPLC and FT-IR microspectrometry to study normal epithelial ovarian cell line immortalized ovarian surface epithelium (IOSE), two epithelial ovarian cell lines (A2780 and CP70) with distinct properties, and the effect of a cancer drug 5-aza-2'-deoxycytidine (5-aza) without labeling. Our results reveal that inhibition of methylation on cytosine with 5-aza initiates the protein expression. Furthermore, paraffin-adsorption kinetic study allows us to distinguish hypermethylated and hypomethyated cells, and this assay can be a potential diagnosis method for cancer screening.
authors.affiliation: Department of Life Science, National Chung Cheng University, Min-Hsiung, Chia-Yi 62102, Taiwan. morris679@gmail.com.
authors.forename: Morris M H
authors.initials: MM
authors.lastname: Hsu
authors.affiliation: National Synchrotron Radiation Research Center, Hsinchu 30076, Taiwan. pyhuang@nsrrc.org.tw.
authors.forename: Pei-Yu
authors.initials: PY
authors.lastname: Huang
authors.affiliation: National Synchrotron Radiation Research Center, Hsinchu 30076, Taiwan. yclee@nsrrc.org.tw.
authors.forename: Yao-Chang
authors.initials: YC
authors.lastname: Lee
authors.affiliation: Department of Life Science, National Chung Cheng University, Min-Hsiung, Chia-Yi 62102, Taiwan. cindy0120811@yahoo.com.tw.
authors.forename: Yuang-Chuen
authors.initials: YC
authors.lastname: Fang
authors.affiliation: Department of Life Science, National Chung Cheng University, Min-Hsiung, Chia-Yi 62102, Taiwan. biowyc@ccu.edu.tw.
authors.forename: Michael W Y
authors.initials: MW
authors.lastname: Chan
authors.affiliation: Department of Life Science, National Chung Cheng University, Min-Hsiung, Chia-Yi 62102, Taiwan. biocil@ccu.edu.tw.
authors.forename: Cheng-I
authors.initials: CI
authors.lastname: Lee
mesh_terms: D001374:Azacitidine / Q000031:analogs & derivatives / Q000633:toxicity; D000077216:Carcinoma, Ovarian Epithelial; D045744:Cell Line, Tumor; D002462:Cell Membrane / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D018899:CpG Islands; D019175:DNA Methylation / Q000187:drug effects; D004275:DNA, Ribosomal / Q000378:metabolism; D000077209:Decitabine; D003843:Deoxycytidine Monophosphate / Q000032:analysis; D057890:Epigenomics; D005260:Female; D006801:Humans; D009375:Neoplasms, Glandular and Epithelial / Q000378:metabolism / Q000473:pathology; D010051:Ovarian Neoplasms / Q000378:metabolism / Q000473:pathology; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D004275:DNA, Ribosomal; D003843:Deoxycytidine Monophosphate; D000077209:Decitabine; D001374:Azacitidine