title: Quantum dot labelling of adenovirus allows highly sensitive single cell flow and imaging cytometry.
abstract: A quantum dot method for highly efficient labelling of single adenoviral particles is developed. The technique has no impact on viral fitness and allows the imaging and tracking of virus binding and internalisation events using a variety of techniques including imaging cytometry and confocal microscopy. The method is applied to characterise the tropism of different adenoviral vectors.
authors.affiliation: School of Molecular and Cellular Biology, Faculty of Biological Sciences, University of Leeds, Leeds, LS2 9JT, UK.
authors.forename: Morgan R
authors.initials: MR
authors.lastname: Herod
authors.forename: Robert G
authors.initials: RG
authors.lastname: Pineda
authors.forename: Vivien
authors.initials: V
authors.lastname: Mautner
authors.forename: David
authors.initials: D
authors.lastname: Onion
mesh_terms: D000256:Adenoviridae / Q000145:classification / Q000378:metabolism*; D019921:Biotinylation; D045744:Cell Line, Tumor; D062727:Coxsackie and Adenovirus Receptor-Like Membrane Protein / Q000378:metabolism; D004705:Endocytosis; D005434:Flow Cytometry / Q000379:methods*; D015316:Genetic Therapy; D006801:Humans; D045663:Quantum Dots / Q000378:metabolism*; D012703:Serotyping; D013194:Staining and Labeling; D018523:Tropism
chemical_list: D062727:Coxsackie and Adenovirus Receptor-Like Membrane Protein
keywords: adenovirus; flow cytometry; gene therapy; imaging cytometry; quantum dots