title: Comparative in vitro Cytotoxic Studies of Novel 8-(4'/2'-Methoxy/Unsubstituted phenylcarbamoyl)bicyclo[3.3.1]nonane Derivatives on Ehrlich Ascites Carcinoma Cell Line.
abstract: Novel bicyclo[3.3.1]nonane derivatives were synthesized by an efficient methodology from acetoacetanilide, 2-methoxy and 4-methoxyacetoacetanilides, 1,3,5-trinitrobenzene and triethylamine. The structures of the compounds were characterized by UV/Visible, FTIR, (1)H NMR and 2D-correlation spectroscopy analysis. The in vitro cytotoxic studies were performed using Ehrlich Ascites Carcinoma cell line by Trypan blue dye exclusion assay and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide cell proliferation assay. The IC50 values of the 8-(4'-/2'-methoxy/unsubstituted phenylcarbamoyl)bicyclo[3.3.1]nonanes were found to be 110.65 μg/ml, 148.23 μg/ml and 151.71 μg/ml, respectively. Thus (4-methoxyphenylcarbomyl)bicyclo[3.3.1]nonane was more potent compared to other two bicyclic adducts.
authors.affiliation: Post Graduate and Research Department of Chemistry, Seethalakshmi Ramaswami College, Tiruchirappalli-620 002, India.
authors.forename: Dhivya
authors.initials: D
authors.lastname: Chandrasekaran
authors.affiliation: Post Graduate and Research Department of Chemistry, Seethalakshmi Ramaswami College, Tiruchirappalli-620 002, India.
authors.forename: S A A
authors.initials: SA
authors.lastname: Vandarkuzhali
authors.affiliation: Department of Biochemistry, Srimad Andavan Arts and Science College, Tiruchirappalli-620 005, India.
authors.forename: G
authors.initials: G
authors.lastname: Sridharan
authors.affiliation: Post Graduate and Research Department of Chemistry, Seethalakshmi Ramaswami College, Tiruchirappalli-620 002, India.
authors.forename: Radha
authors.initials: R
authors.lastname: Natarajan
authors.affiliation: Centre for Advanced Research in Indian System of Medicine (CARISM), Sastra University, Thanjavur-613 401, India.
authors.forename: P
authors.initials: P
authors.lastname: Brindha
keywords: Bicyclo[3.3.1]nonanes; Ehrlich Ascites Carcinoma cell line; MTT assay; methoxy group activity; trypan blue