title: Distinct spatial relationship of the interleukin-9 receptor with interleukin-2 receptor and major histocompatibility complex glycoproteins in human T lymphoma cells.
abstract: The interleukin-9 receptor (IL-9R) consists of an α subunit and a γ(c) chain that are shared with other cytokine receptors, including interleukin-2 receptor (IL-2R), an important regulator of T cells. We previously showed that IL-2R is expressed in common clusters with major histocompatibility complex (MHC) glycoproteins in lipid rafts of human T lymphoma cells, which raised the question about what the relationship between clusters of IL-2R/MHC and IL-9R is. Confocal microscopy colocalization and fluorescence resonance energy transfer experiments capable of detecting membrane protein organization at different size scales revealed nonrandom association of IL-9R with IL-2R/MHC clusters at the surface of human T lymphoma cells. Accommodation of IL-9Rα in membrane areas segregated from the IL-2R/MHC domains was also detected. The bipartite nature of IL-9R distribution was mirrored by signal transducer and activator of transcription (STAT) activation results. Our data indicate that co-compartmentalization with MHC glycoproteins is a general property of γ(c) receptors. Distribution of receptor chains between different membrane domains may regulate their function.
authors.affiliation: Department of Biophysics and Cell Biology, Research Center for Molecular Medicine, University of Debrecen, P.O.B. 39., 4012, Debrecen (Hungary).
authors.forename: Enikő
authors.initials: E
authors.lastname: Nizsalóczki
authors.forename: István
authors.initials: I
authors.lastname: Csomós
authors.forename: Péter
authors.initials: P
authors.lastname: Nagy
authors.forename: Zsolt
authors.initials: Z
authors.lastname: Fazekas
authors.forename: Carolyn K
authors.initials: CK
authors.lastname: Goldman
authors.forename: Thomas A
authors.initials: TA
authors.lastname: Waldmann
authors.forename: Sándor
authors.initials: S
authors.lastname: Damjanovich
authors.forename: György
authors.initials: G
authors.lastname: Vámosi
authors.forename: László
authors.initials: L
authors.lastname: Mátyus
authors.forename: Andrea
authors.initials: A
authors.lastname: Bodnár
mesh_terms: D045744:Cell Line, Tumor; D031541:Fluorescence Resonance Energy Transfer; D006023:Glycoproteins / Q000032:analysis*; D006680:HLA Antigens / Q000032:analysis*; D006801:Humans; D016399:Lymphoma, T-Cell / Q000473:pathology*; D008285:Major Histocompatibility Complex; D018613:Microscopy, Confocal; D015375:Receptors, Interleukin-2 / Q000032:analysis*; D053651:Receptors, Interleukin-9 / Q000032:analysis*; D013601:T-Lymphocytes / Q000737:chemistry / Q000473:pathology*
chemical_list: D006023:Glycoproteins; D006680:HLA Antigens; D015375:Receptors, Interleukin-2; D053651:Receptors, Interleukin-9
keywords: FRET; confocal microscopy; membrane proteins; protein-protein interactions; receptors