title: Capillary electrophoresis separation of aminoalkanol derivatives of 1,7-dimethyl-8,9-diphenyl-4-azatricyclo[5.2.1.0(2,6)]dec-8-ene-3,5,10-trione as potential anticancer drugs.
abstract: The purpose of this study, the direct separation of aminoalkanol derivatives I and II of 1,7-dimethyl-8,9-diphenyl-4-azatricyclo[5.2.1.0(2,6) ]dec-8-ene-3,5,10-trione, which was found in earlier studies as potential anticancer drugs, were performed. Capillary electrophoresis offers the possibility of fast, cheap, and reproducible separations for compounds I and II. In this paper, the simultaneous separation of I and II by capillary zone electrophoresis has been achieved within 8 min by use of 50 mM phosphate buffer of pH 2.5. Analysis of the two compounds in the serum plasma standards was conducted. Limits of detection of I and II by UV absorbance at 200 nm were achieved in the range of 156.3-156.6 ng/mL. The method was validated for linearity, accuracy, precision, limits of detection, and quantification. The calibration equation revealed a good linear relationship (r(2) = 0.998-0.999). Sufficient recovery was observed in the range of 96.3-99.5%. The method showed good reproducibility with intra- and interday precision of 0.97 and 1.76%, respectively. The quantification limits for the compounds were in the range of 477.0-479.8 ng/mL. The proposed method was applied to the analysis of real serum samples.
authors.affiliation: Chair and Department of Biochemistry and Clinical Chemistry, Medical University of Warsaw, Warsaw, Poland.
authors.forename: Błażej
authors.initials: B
authors.lastname: Grodner
authors.forename: Jacek
authors.initials: J
authors.lastname: Łukaszkiewicz
authors.forename: Bożena
authors.initials: B
authors.lastname: Kuran
authors.forename: Mariola
authors.initials: M
authors.lastname: Krawiecka
mesh_terms: D000970:Antineoplastic Agents / Q000097:blood / Q000302:isolation & purification*; D019075:Electrophoresis, Capillary / Q000379:methods*; D006575:Heterocyclic Compounds, 3-Ring / Q000097:blood / Q000302:isolation & purification*; D006801:Humans; D007094:Imides / Q000097:blood / Q000302:isolation & purification*; D015394:Molecular Structure; D012680:Sensitivity and Specificity
chemical_list: C000599453:4-(2-hydroxy-3-(propan-2-ylamino)propyl)-1,7-dimethyl-8,9-diphenyl-4-azatricyclo(5.2.1.0(2,6))dec-8-ene-3,5,10-trione; C000599454:4-(3-(dimethylamino)-2-hydroxypropyl)-1,7-dimethyl-8,9-diphenyl-4-azatricyclo(5.2.1.0(2,6))dec-8-ene-3,5,10-trione; D000970:Antineoplastic Agents; D006575:Heterocyclic Compounds, 3-Ring; D007094:Imides
keywords: Anticancer drugs; Capillary electrophoresis; Drug monitoring