title: Wound Healing Effect of Conditioned Media Obtained From Adipose Tissue on Human Skin Cells: A Comparative in Vitro Study.
abstract: BACKGROUND
  Split-thickness skin grafting is the gold standard to cover extensive acute and chronic wounds with a well-vascularized wound bed. Although some headway has been made in developing biological agents to speed up healing, there is still no treatment that sufficiently replaces skin grafts to date. The use of secretory factors of adipose tissue may be a feasible approach to developing topical wound applications for faster wound healing.
  
  
  METHODS
  In this study, the effect of conditioned media (CMs) of human adipose-derived stem cells (ASCs), adipocytes, or adipose tissue on human skin cells was evaluated for viability, proliferation, and migration in vitro. Differentiation potential of stem cells treated with CM was monitored by AdipoRed staining and qualitative real-time polymerase chain reaction. Angiogenic potential of human endothelial cells treated with CM was tested via sprouting assay.
  
  
  RESULTS
  The CM of adipose tissue significantly enhanced ASC proliferation (P < 0.01). Treatment with CM showed no inductive effect on ASC differentiation into adipocytes but, at the same time, significantly induced cell sprouting of endothelial cells (P < 0.001). We show for the first time that CM of adipose tissue is a potent inducer of proliferation of ASCs and angiogenesis, with comparable effects with those of stem cell-enriched CM.
  
  
  CONCLUSIONS
  We suggest the use of the secretome of adipose tissue to produce CM for topical application on wounds, rather than working with adipose tissue or including the difficult process of enriching the patients' stem cells in vitro.
authors.affiliation: From the *Division of Plastic and Reconstructive Surgery, Department of Surgery; †Christian Doppler-Laboratory for Cardio-Metabolic Immunotherapy at the Clinical Division of Endocrinology and Metabolism, Department of Medicine III; ‡Department of Surgery; and §Division of Thoracic Surgery, Department of Surgery, Medical University of Vienna; and ∥Christian Doppler Laboratory for Cardiac and Thoracic Diagnosis and Regeneration, Vienna, Austria.
authors.forename: Johanna
authors.initials: J
authors.lastname: Kober
authors.forename: Alfred
authors.initials: A
authors.lastname: Gugerell
authors.forename: Melanie
authors.initials: M
authors.lastname: Schmid
authors.forename: Maximilian
authors.initials: M
authors.lastname: Zeyda
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Buchberger
authors.forename: Stefanie
authors.initials: S
authors.lastname: Nickl
authors.forename: Stefan
authors.initials: S
authors.lastname: Hacker
authors.forename: Hendrik Jan
authors.initials: HJ
authors.lastname: Ankersmit
authors.forename: Maike
authors.initials: M
authors.lastname: Keck
mesh_terms: D017667:Adipocytes / Q000502:physiology*; D002454:Cell Differentiation; D002465:Cell Movement; D049109:Cell Proliferation; D017077:Culture Media, Conditioned; D004847:Epithelial Cells / Q000502:physiology*; D005347:Fibroblasts / Q000502:physiology*; D006801:Humans; D066298:In Vitro Techniques; D015603:Keratinocytes / Q000502:physiology*; D059630:Mesenchymal Stem Cells / Q000502:physiology*; D018919:Neovascularization, Physiologic; D050151:Subcutaneous Fat / Q000166:cytology*; D014945:Wound Healing / Q000502:physiology*
chemical_list: D017077:Culture Media, Conditioned