title: 3D registration of micro-PET-CT for measurable correlates of dyspeptic symptoms in mice.
abstract: Patients with chronic calorie insufficiency commonly suffer from upper gastrointestinal dysfunction and consequent dyspeptic symptoms, which may interfere with their nutritional rehabilitation. To investigate the relationship between gastric dysfunction and feeding behavior, we exposed mice to chronic caloric restriction and demonstrated gastric motor abnormalities in them. Gastric dysmotility is typically associated with dyspeptic symptoms but sensations cannot be directly assessed in animal models. Therefore, as an initial step toward establishing measurable correlates of postprandial symptoms in small animals, we have attempted to characterize central responses to food intake by positron emission tomography-computerized microtomography (PET-CT) in normal and calorically restricted mice. Animals consumed a standard test meal after an overnight fast before receiving 2-deoxy-2[18F]fluoro-D-glucose tracer. The same mice were also scanned in the fasting state on a separate day. We were able to bring the fed and fasting PET volume images into spatial registration with each other and with an MR-derived atlas of the mouse brain, so that the differences in uptake between the two states could be mapped quantitatively against the neuroanatomic regions of the atlas. Our approach is suitable for studying the effects of gastric dysmotilities on central responses to feeding.
authors.affiliation: Biomedical Imaging Resource, Mayo Clinic/Foundation, 200 1 ST. SW Rochester, MN 55905.
authors.forename: Jon
authors.initials: J
authors.lastname: Camp
authors.affiliation: Biomedical Imaging Resource, Mayo Clinic/Foundation, 200 1 ST. SW Rochester, MN 55905.
authors.forename: Kathryn
authors.initials: K
authors.lastname: Simpson
authors.affiliation: Enteric Neuroscience Program, Miles and Shirley Fiterman Center for Digestive Diseases and Department of Physiology and Biomedical Engineering, Mayo Clinic/Foundation, 200 1 ST. SW Rochester, MN 55905.
authors.forename: Michael R
authors.initials: MR
authors.lastname: Bardsley
authors.affiliation: Enteric Neuroscience Program, Miles and Shirley Fiterman Center for Digestive Diseases and Department of Physiology and Biomedical Engineering, Mayo Clinic/Foundation, 200 1 ST. SW Rochester, MN 55905.
authors.forename: Laura N
authors.initials: LN
authors.lastname: Popko
authors.affiliation: Enteric Neuroscience Program, Miles and Shirley Fiterman Center for Digestive Diseases and Department of Physiology and Biomedical Engineering, Mayo Clinic/Foundation, 200 1 ST. SW Rochester, MN 55905.
authors.forename: David L
authors.initials: DL
authors.lastname: Young
authors.affiliation: Dept. of Nuclear Medicine, Mayo Clinic/Foundation, 200 1 ST. SW Rochester, MN 55905.
authors.forename: Bradley J
authors.initials: BJ
authors.lastname: Kemp
authors.affiliation: Dept. of Nuclear Medicine, Mayo Clinic/Foundation, 200 1 ST. SW Rochester, MN 55905.
authors.forename: Val
authors.initials: V
authors.lastname: Lowe
authors.affiliation: Enteric Neuroscience Program, Miles and Shirley Fiterman Center for Digestive Diseases and Department of Physiology and Biomedical Engineering, Mayo Clinic/Foundation, 200 1 ST. SW Rochester, MN 55905.
authors.forename: Tamas
authors.initials: T
authors.lastname: Ordog
authors.affiliation: Biomedical Imaging Resource, Mayo Clinic/Foundation, 200 1 ST. SW Rochester, MN 55905.
authors.forename: Richard
authors.initials: R
authors.lastname: Robb
keywords: anorexia nervosa; bulimia nervosa; caloric restriction; dyspepsia; micro-CT; micro-PET; registration