title: Functional ultrasound imaging of intrinsic connectivity in the living rat brain with high spatiotemporal resolution.
abstract: Long-range coherences in spontaneous brain activity reflect functional connectivity. Here we propose a novel, highly resolved connectivity mapping approach, using ultrafast functional ultrasound (fUS), which enables imaging of cerebral microvascular haemodynamics deep in the anaesthetized rodent brain, through a large thinned-skull cranial window, with pixel dimensions of 100 μm × 100 μm in-plane. The millisecond-range temporal resolution allows unambiguous cancellation of low-frequency cardio-respiratory noise. Both seed-based and singular value decomposition analysis of spatial coherences in the low-frequency (<0.1 Hz) spontaneous fUS signal fluctuations reproducibly report, at different coronal planes, overlapping high-contrast, intrinsic functional connectivity patterns. These patterns are similar to major functional networks described in humans by resting-state fMRI, such as the lateral task-dependent network putatively anticorrelated with the midline default-mode network. These results introduce fUS as a powerful novel neuroimaging method, which could be extended to portable systems for three-dimensional functional connectivity imaging in awake and freely moving rodents.
authors.affiliation: 1] Institut Langevin, ESPCI-ParisTech, 1 rue Cuvier, 75005 Paris, France [2] CNRS UMR 7587, 1 rue Cuvier, 75005 Paris, France [3] INSERM U979 'Wave Physics for Medicine' Lab, 1 rue Cuvier, 75005 Paris, France.
authors.forename: Bruno-Félix
authors.initials: BF
authors.lastname: Osmanski
authors.affiliation: 1] Centre National pour la Recherche Scientifique, UMR 8249, 10 rue Vauquelin, 75005 Paris, France [2] Brain Plasticity Unit, ESPCI-ParisTech, 10 rue Vauquelin, 75005 Paris, France.
authors.forename: Sophie
authors.initials: S
authors.lastname: Pezet
authors.affiliation: 1] Centre National pour la Recherche Scientifique, UMR 8249, 10 rue Vauquelin, 75005 Paris, France [2] Brain Plasticity Unit, ESPCI-ParisTech, 10 rue Vauquelin, 75005 Paris, France.
authors.forename: Ana
authors.initials: A
authors.lastname: Ricobaraza
authors.affiliation: 1] Centre National pour la Recherche Scientifique, UMR 8249, 10 rue Vauquelin, 75005 Paris, France [2] Brain Plasticity Unit, ESPCI-ParisTech, 10 rue Vauquelin, 75005 Paris, France.
authors.forename: Zsolt
authors.initials: Z
authors.lastname: Lenkei
authors.affiliation: 1] Institut Langevin, ESPCI-ParisTech, 1 rue Cuvier, 75005 Paris, France [2] CNRS UMR 7587, 1 rue Cuvier, 75005 Paris, France [3] INSERM U979 'Wave Physics for Medicine' Lab, 1 rue Cuvier, 75005 Paris, France.
authors.forename: Mickael
authors.initials: M
authors.lastname: Tanter
mesh_terms: D000818:Animals; D001921:Brain / Q000502:physiology*; D001931:Brain Mapping / Q000379:methods*; D003287:Contrast Media / Q000737:chemistry; D004453:Echoencephalography; D006439:Hemodynamics; D007091:Image Processing, Computer-Assisted; D008279:Magnetic Resonance Imaging; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D003287:Contrast Media