title: A high-resolution LC-MS-based secondary metabolite fingerprint database of marine bacteria.
abstract: Marine bacteria are the most widely distributed organisms in the ocean environment and produce a wide variety of secondary metabolites. However, traditional screening for bioactive natural compounds is greatly hindered by the lack of a systematic way of cataloguing the chemical profiles of bacterial strains found in nature. Here we present a chemical fingerprint database of marine bacteria based on their secondary metabolite profiles, acquired by high-resolution LC-MS. Till now, 1,430 bacterial strains spanning 168 known species collected from different marine environments were cultured and profiled. Using this database, we demonstrated that secondary metabolite profile similarity is approximately, but not always, correlated with taxonomical similarity. We also validated the ability of this database to find species-specific metabolites, as well as to discover known bioactive compounds from previously unknown sources. An online interface to this database, as well as the accompanying software, is provided freely for the community to use.
authors.affiliation: 1] Environmental Science Program, School of Science, Hong Kong University of Science and Technology, Clear Water Bay, Hong Kong 999077, China [2].
authors.forename: Liang
authors.initials: L
authors.lastname: Lu
authors.affiliation: 1] Division of Biomedical Engineering, Hong Kong University of Science and Technology, Clear Water Bay, Hong Kong 999077, China [2].
authors.forename: Jijie
authors.initials: J
authors.lastname: Wang
authors.affiliation: Division of Life Science, Hong Kong University of Science and Technology, Clear Water Bay, Hong Kong 999077, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Xu
authors.affiliation: School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, China.
authors.forename: Kailing
authors.initials: K
authors.lastname: Wang
authors.affiliation: Department of Chemical and Biomolecular Engineering, Hong Kong University of Science and Technology, Clear Water Bay, Hong Kong 999077, China.
authors.forename: Yingwei
authors.initials: Y
authors.lastname: Hu
authors.affiliation: Environmental Science Program, School of Science, Hong Kong University of Science and Technology, Clear Water Bay, Hong Kong 999077, China.
authors.forename: Renmao
authors.initials: R
authors.lastname: Tian
authors.affiliation: Division of Life Science, Hong Kong University of Science and Technology, Clear Water Bay, Hong Kong 999077, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Yang
authors.affiliation: Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, China.
authors.forename: Qiliang
authors.initials: Q
authors.lastname: Lai
authors.affiliation: Division of Life Science, Hong Kong University of Science and Technology, Clear Water Bay, Hong Kong 999077, China.
authors.forename: Yongxin
authors.initials: Y
authors.lastname: Li
authors.affiliation: Environmental Science Program, School of Science, Hong Kong University of Science and Technology, Clear Water Bay, Hong Kong 999077, China.
authors.forename: Weipeng
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, China.
authors.forename: Zongze
authors.initials: Z
authors.lastname: Shao
authors.affiliation: 1] Division of Biomedical Engineering, Hong Kong University of Science and Technology, Clear Water Bay, Hong Kong 999077, China [2] Department of Chemical and Biomolecular Engineering, Hong Kong University of Science and Technology, Clear Water Bay, Hong Kong 999077, China.
authors.forename: Henry
authors.initials: H
authors.lastname: Lam
authors.affiliation: 1] Environmental Science Program, School of Science, Hong Kong University of Science and Technology, Clear Water Bay, Hong Kong 999077, China [2] Division of Life Science, Hong Kong University of Science and Technology, Clear Water Bay, Hong Kong 999077, China.
authors.forename: Pei-Yuan
authors.initials: PY
authors.lastname: Qian
mesh_terms: D059001:Aquatic Organisms / Q000737:chemistry* / Q000254:growth & development / Q000378:metabolism; D001419:Bacteria / Q000737:chemistry* / Q000378:metabolism; D002853:Chromatography, Liquid; D016172:DNA Fingerprinting; D030541:Databases, Genetic; D013058:Mass Spectrometry; D055442:Metabolome