title: Chemically-induced redox switching of a metalloprotein reveals thermodynamic and kinetic heterogeneity, one molecule at a time.
abstract: Oxidation (off state) and reduction (on state) of a single azurin molecule is monitored, one electron at a time, which depend on the chemical redox potential. By analysing the fluorescence time traces from individual azurin molecules, reaction kinetics and redox thermodynamics were determined.
authors.affiliation: Biological and Soft Matter Physics, LION, Leiden University, Niels Bohrweg 2, 2333 CA Leiden, The Netherlands. namikakkilic@gmail.com.
authors.forename: Namik
authors.initials: N
authors.lastname: Akkilic
authors.forename: Fenna
authors.initials: F
authors.lastname: van der Grient
authors.forename: Muhammad
authors.initials: M
authors.lastname: Kamran
authors.forename: Nusrat J M
authors.initials: NJ
authors.lastname: Sanghamitra
mesh_terms: D001400:Azurin / Q000737:chemistry*; D004583:Electrons; D005453:Fluorescence; D007700:Kinetics; D010084:Oxidation-Reduction; D013816:Thermodynamics; D013997:Time Factors
chemical_list: D001400:Azurin