title: Inexpensive diffuse reflectance spectroscopy system for measuring changes in tissue optical properties.
abstract: The measurement of changes in blood volume in tissue is important for monitoring the effects of a wide range of therapeutic interventions, from radiation therapy to skin-flap transplants. Many systems available for purchase are either expensive or difficult to use, limiting their utility in the clinical setting. A low-cost system, capable of measuring changes in tissue blood volume via diffuse reflectance spectroscopy is presented. The system consists of an integrating sphere coupled via optical fibers to a broadband light source and a spectrometer. Validation data are presented to illustrate the accuracy and reproducibility of the system. The validity and utility of this in vivo system were demonstrated in a skin blanching/reddening experiment using epinephrine and lidocaine, and in a study measuring the severity of radiation-induced erythema during radiation therapy.
authors.affiliation: McMaster University, Department of Medical Physics and Applied Radiation Sciences, 1280 Main Street West, Hamilton, Ontario L8S 4L8, Canada.
authors.forename: Diana L
authors.initials: DL
authors.lastname: Glennie
authors.affiliation: McMaster University, Department of Medical Physics and Applied Radiation Sciences, 1280 Main Street West, Hamilton, Ontario L8S 4L8, CanadabJuravinski Cancer Centre, Department of Medical Physics, 699 Concession Street, Hamilton, Ontario L8V 5C2, Canada.
authors.forename: Joseph E
authors.initials: JE
authors.lastname: Hayward
authors.affiliation: McMaster University, Department of Surgery, Division of Plastic and Reconstructive Surgery, 1280 Main Street West, Hamilton, Ontario L8S 4L8, Canada.
authors.forename: Daniel E
authors.initials: DE
authors.lastname: McKee
authors.affiliation: McMaster University, Department of Medical Physics and Applied Radiation Sciences, 1280 Main Street West, Hamilton, Ontario L8S 4L8, CanadabJuravinski Cancer Centre, Department of Medical Physics, 699 Concession Street, Hamilton, Ontario L8V 5C2, Canada.
authors.forename: Thomas J
authors.initials: TJ
authors.lastname: Farrell
mesh_terms: D004837:Epinephrine / Q000494:pharmacology; D004890:Erythema / Q000473:pathology; D006801:Humans; D008012:Lidocaine / Q000494:pharmacology; D061848:Optical Imaging / Q000379:methods*; D012867:Skin / Q000737:chemistry* / Q000187:drug effects; D013057:Spectrum Analysis / Q000379:methods*
chemical_list: D008012:Lidocaine; D004837:Epinephrine