title: β-cateninknockdown enhances the effects of fluorouracil in the breast cancer cell line MDA-MB-468.
abstract: Tumor proliferation, drug resistance and cell stemness are major difficulties that are encountered during breast cancer therapy and are often responsible for disease progression and cancer-related mortality.β-cateninis considered to be an invasion gene in breast cancer. However, howβ-cateninregulates breast cancer cell proliferation and stemness remains unclear. In the present study,β-cateninknockdown by small interfering RNA in MDA-MB-468, a highly metastatic breast cancer cell line, inhibited the expression ofβ-catenin,Oct3/4(stemness),survivin(anti-apoptosis) andBCRP(drug resistance). Knockdown ofβ-cateninenhanced the effects of fluorouracil (5-FU) chemotherapy on the proliferation of MDA-MB-468 cells. Thus, these preliminary results indicate thatβ-cateninknockdown enhanced 5-FU-induced proliferation inhibition in the breast cancer cell line MDA-MB-468, and indicate that combining 5-FU with gene silencing could be an advantageous option for enhancing the curative effect of chemotherapy in breast cancer and other malignancies.
authors.affiliation: Department of Pathology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, P.R. China.
authors.forename: Xinquan
authors.initials: X
authors.lastname: Lv
authors.affiliation: Department of Pathology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, P.R. China.
authors.forename: Xia
authors.initials: X
authors.lastname: Pang
authors.affiliation: Department of Pathology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, P.R. China.
authors.forename: Xiangdong
authors.initials: X
authors.lastname: Jin
authors.affiliation: Department of Pathology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, P.R. China.
authors.forename: Yimin
authors.initials: Y
authors.lastname: Song
authors.affiliation: Department of Pathology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, P.R. China.
authors.forename: Huixiang
authors.initials: H
authors.lastname: Li
keywords: RNA interference; breast cancer; gene therapy; stem cell; β-catenin