title: Survival and fate of Salmonella enterica serovar Montevideo in adult horn flies (Diptera: Muscidae).
abstract: Contamination of cattle peripheral lymph nodes with Salmonella enterica is proposed to occur via a transdermal route of entry. If so, bacteria may be introduced to cattle by biting arthropods. Biting flies, such as horn flies (Haematobia irritans irritans (L.)) (Diptera: Muscidae), are intriguing candidates for transmitting Salmonella to cattle because they provide a route of entry when they breach the skin barrier during blood feeding. Using a green fluorescent protein-expressing strain of Salmonella Montevideo (S. Montevideo-GFP), the current study demonstrated that horn fly grooming subsequent to tactile exposure to the bacteria resulted in acquisition of the bacteria on mouthparts as well as microbial ingestion. Consumption of a bloodmeal containing approximately 10(2), approximately 10(4), or 10(6) S. Montevideo-GFP resulted in horn fly colonization for up to 72 h postingestion (PI). Epifluorescent microscopy indicated that the bacteria were not localized to the crop but were observed within the endoperitrophic space, suggesting that regurgitation is not a primary route of transmission. S. Montevideo-GFP were cultured from excreta of 100% of flies beginning 6-7 h PI of a medium or high dose meal and > 12 h PI in excreta from 60% of flies fed the low-dose meal. Animal hides and manure pats are sources for horn flies to acquire the Salmonella and mechanically transmit them to an animal while feeding. Mean quantities of 5.65-67.5 x 10(2) CFU per fly were cultured from fly excreta passed within 1 d after feeding, suggesting the excreta can provide an additional microbial source on the animal's hide.
authors.forename: Pia Untalan
authors.initials: PU
authors.lastname: Olafson
authors.forename: Kimberly H
authors.initials: KH
authors.lastname: Lohmeyer
authors.forename: Thomas S
authors.initials: TS
authors.lastname: Edrington
authors.forename: Guy H
authors.initials: GH
authors.lastname: Loneragan
mesh_terms: D000818:Animals; D041981:Gastrointestinal Tract / Q000382:microbiology; D009117:Muscidae / Q000382:microbiology*; D019779:Salmonella enterica / Q000145:classification* / Q000502:physiology*