title: Expression of ezrin and moesin related to invasion, metastasis and prognosis of laryngeal squamous cell carcinoma.
abstract: We examined the expression of ezrin and moesin in laryngeal squamous cell carcinoma (LSCC) and their correlation with patient clinicopathological characteristics and overall survival. Immunohistochemical staining and reverse transcription-polymerase chain reaction (RT-PCR) for ezrin and moesin were applied to 60 carcinoma tissues, adjacent normal tissues, and 33 metastatic lymph nodes. Survival functions were estimated using the Kaplan-Meier method and compared by the log-rank test. RT-PCR demonstrated that the intensity ratios of ezrin and moesin to β-actin were higher in LSCC than in adjacent normal mucous membrane (P < 0.05). Furthermore, intensity ratios were higher in cervical metastatic lymph nodes than in LSCC (P < 0.05). Immunohistochemical staining showed that ezrin and moesin were well distributed in the cell membrane and cytoplasm. Expression was significantly different between LSCC and adjacent normal tissues (P < 0.05); moreover, expression in the cervical metastatic lymph nodes was higher than in LSCC (P < 0.05). Expression of ezrin and moesin was significantly related to clinical stage, T stage, and cervical lymph node metastasis (P < 0.05), except that moesin showed no significant relationship with clinical stage (P > 0.05). Patients with negative ezrin and moesin expression had a significantly longer overall survival time compared to patients with moderate and intense ezrin and moesin expression (P < 0.001, P < 0.05). Ezrin and moesin expression is related to LSCC invasion and metastasis, and may be important molecular markers for predicting prognosis and therapeutic targets in LSCC patients.
authors.affiliation: Department of Otorhinolaryngology & Head and Neck Surgery, Second Hospital Affiliated to Harbin Medical University, Harbin, China.
authors.forename: X
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Otorhinolaryngology & Head and Neck Surgery, Second Hospital Affiliated to Harbin Medical University, Harbin, China.
authors.forename: M
authors.initials: M
authors.lastname: Liu
authors.affiliation: Department of Otorhinolaryngology & Head and Neck Surgery, Second Hospital Affiliated to Harbin Medical University, Harbin, China chunyuanzhao@yeah.net.
authors.forename: C Y
authors.initials: CY
authors.lastname: Zhao
mesh_terms: D002294:Carcinoma, Squamous Cell / Q000378:metabolism / Q000473:pathology*; D003598:Cytoskeletal Proteins / Q000235:genetics / Q000378:metabolism*; D005260:Female; D006801:Humans; D007822:Laryngeal Neoplasms / Q000378:metabolism / Q000473:pathology*; D008297:Male; D008840:Microfilament Proteins / Q000235:genetics / Q000378:metabolism*; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis; D011379:Prognosis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D000099149:Ezrin
chemical_list: D003598:Cytoskeletal Proteins; D008840:Microfilament Proteins; D000099149:Ezrin; C071058:moesin