title: Directed Evolution of a Panel of Orthogonal T7 RNA Polymerase Variants for in Vivo or in Vitro Synthetic Circuitry.
abstract: T7 RNA polymerase is the foundation of synthetic biological circuitry both in vivo and in vitro due to its robust and specific control of transcription from its cognate promoter. Here we present the directed evolution of a panel of orthogonal T7 RNA polymerase:promoter pairs that each specifically recognizes a synthetic promoter. These newly described pairs can be used to independently control up to six circuits in parallel.
authors.affiliation: Institute for Cellular and Molecular Biology, ‡Department of Chemistry and Biochemistry, and §Center for Systems & Synthetic Biology, University of Texas at Austin , Austin, Texas 78712, United States.
authors.forename: Adam J
authors.initials: AJ
authors.lastname: Meyer
authors.affiliation: Institute for Cellular and Molecular Biology, ‡Department of Chemistry and Biochemistry, and §Center for Systems & Synthetic Biology, University of Texas at Austin , Austin, Texas 78712, United States.
authors.forename: Jared W
authors.initials: JW
authors.lastname: Ellefson
authors.affiliation: Institute for Cellular and Molecular Biology, ‡Department of Chemistry and Biochemistry, and §Center for Systems & Synthetic Biology, University of Texas at Austin , Austin, Texas 78712, United States.
authors.forename: Andrew D
authors.initials: AD
authors.lastname: Ellington
mesh_terms: D012321:DNA-Directed RNA Polymerases / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics*; D014764:Viral Proteins / Q000235:genetics*
chemical_list: D014764:Viral Proteins; C062210:bacteriophage T7 RNA polymerase; D012321:DNA-Directed RNA Polymerases
keywords: directed evolution; genetic circuits; transcription