title: Inhibition of PPARα attenuates vimentin phosphorylation on Ser-83 and collapse of vimentin filaments during exposure of rat Sertoli cells in vitro to DBP.
abstract: Dibutyl phthalate (DBP) is a peroxisome proliferator which can lead to germ cell loss from Sertoli cells. Collapse of vimentin filaments occurs in Sertoli cells after DBP exposure. Peroxisome proliferator activated receptor α (PPARα) is a key receptor which could be activated by DBP. The role of PPARα in this process was investigated. Results showed that, PPARα was activated in DBP-exposed Sertoli cells, GW6471 inhibited the activity of PPARα, phosphorylation level of vimentin and concentration of soluble vimentin was higher in DBP-treated Sertoli cells than GW6471+DBP-treated cells. These results suggest that PPARα directly or indirectly mediated phosphorylation of vimentin on Ser 83, and PPARα may play an important role in regulating the reorganization of vimentin filaments during exposure of Sertoli cells to DBP.
authors.affiliation: Toxicology Institute, College of Preventive Medicine, Third Military Medical University, No. 30, Gaotanyan Road, Shapingba District, Chongqing 400038, China. Electronic address: zxzx126@163.com.
authors.forename: Xi
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Toxicology Institute, College of Preventive Medicine, Third Military Medical University, No. 30, Gaotanyan Road, Shapingba District, Chongqing 400038, China. Electronic address: wenbinliutmmu@sina.com.
authors.forename: Wenbin
authors.initials: W
authors.lastname: Liu
authors.affiliation: Toxicology Institute, College of Preventive Medicine, Third Military Medical University, No. 30, Gaotanyan Road, Shapingba District, Chongqing 400038, China. Electronic address: ouchyoung@gmail.com.
authors.forename: Huan
authors.initials: H
authors.lastname: Yang
authors.affiliation: Toxicology Institute, College of Preventive Medicine, Third Military Medical University, No. 30, Gaotanyan Road, Shapingba District, Chongqing 400038, China. Electronic address: Tanlubnu@gmail.com.
authors.forename: Lu
authors.initials: L
authors.lastname: Tan
authors.affiliation: Toxicology Institute, College of Preventive Medicine, Third Military Medical University, No. 30, Gaotanyan Road, Shapingba District, Chongqing 400038, China. Electronic address: aolin117@163.com.
authors.forename: Lin
authors.initials: L
authors.lastname: Ao
authors.affiliation: Toxicology Institute, College of Preventive Medicine, Third Military Medical University, No. 30, Gaotanyan Road, Shapingba District, Chongqing 400038, China. Electronic address: jinyiliutmmu@163.com.
authors.forename: Jinyi
authors.initials: J
authors.lastname: Liu
authors.affiliation: Toxicology Institute, College of Preventive Medicine, Third Military Medical University, No. 30, Gaotanyan Road, Shapingba District, Chongqing 400038, China. Electronic address: caojia1962@126.com.
authors.forename: Jia
authors.initials: J
authors.lastname: Cao
authors.affiliation: Toxicology Institute, College of Preventive Medicine, Third Military Medical University, No. 30, Gaotanyan Road, Shapingba District, Chongqing 400038, China. Electronic address: zhihongcui_4@126.com.
authors.forename: Zhihong
authors.initials: Z
authors.lastname: Cui
mesh_terms: D000818:Animals; D003993:Dibutyl Phthalate / Q000633:toxicity*; D008297:Male; D047493:PPAR alpha / Q000037:antagonists & inhibitors* / Q000502:physiology; D010766:Phosphorylation; D051381:Rats; D017207:Rats, Sprague-Dawley; D012694:Serine / Q000378:metabolism; D012708:Sertoli Cells / Q000187:drug effects*; D014746:Vimentin / Q000378:metabolism*
chemical_list: D047493:PPAR alpha; D014746:Vimentin; D003993:Dibutyl Phthalate; D012694:Serine
keywords: Dibutyl phthalate; PPARα; Phosphorylation; Sertoli cells; Vimentin