title: Capsular Serotyping of Streptococcus pneumoniae by latex agglutination.
abstract: Latex agglutination reagents are widely used in microbial diagnosis, identification and serotyping. Streptococcus pneumoniae (the pneumococcus) is a major cause of morbidity and mortality world-wide. Current vaccines target the pneumococcal capsule, and there are over 90 capsular serotypes. Serotyping pneumococcal isolates is therefore important for assessing the impact of vaccination programs and for epidemiological purposes. The World Health Organization has recommended latex agglutination as an alternative method to the 'gold standard' Quellung test for serotyping pneumococci. Latex agglutination is a relatively simple, quick and inexpensive method; and is therefore suitable for resource-poor settings as well as laboratories with high-volume workloads. Latex agglutination reagents can be prepared in-house utilizing commercially-sourced antibodies that are passively attached to latex particles. This manuscript describes a method of production and quality control of latex agglutination reagents, and details a sequential testing approach which is time- and cost-effective. This method of production and quality control may also be suitable for other testing purposes.
authors.affiliation: Pneumococcal Research, Murdoch Childrens Research Institute.
authors.forename: Barbara D
authors.initials: BD
authors.lastname: Porter
authors.affiliation: Pneumococcal Research, Murdoch Childrens Research Institute.
authors.forename: Belinda D
authors.initials: BD
authors.lastname: Ortika
authors.affiliation: Pneumococcal Research, Murdoch Childrens Research Institute; Department of Microbiology and Immunology, Peter Doherty Institute for Infection and Immunity, The University of Melbourne; catherine.satzke@mcri.edu.au.
authors.forename: Catherine
authors.initials: C
authors.lastname: Satzke
mesh_terms: D000818:Animals; D016667:Bacterial Capsules / Q000145:classification; D006801:Humans; D007841:Latex Fixation Tests / Q000379:methods* / Q000592:standards; D012703:Serotyping / Q000379:methods* / Q000592:standards; D013296:Streptococcus pneumoniae / Q000145:classification*