title: Response to drought and salt stress in leaves of poplar (Populus alba × Populus glandulosa): expression profiling by oligonucleotide microarray analysis.
abstract: Drought and salt stresses are major environmental constraints on forest productivity. To identify genes responsible for stress tolerance, we conducted a genome-wide analysis in poplar (Populus alba × Populus glandulosa) leaves exposed to drought and salt (NaCl) stresses. We investigated gene expression at the mRNA level using oligonucleotide microarrays containing 44,718 genes from Populus trichocarpa. A total of 1604 and 1042 genes were up-regulated (≥2-fold; P value < 0.05) by drought and salt stresses, respectively, and 765 genes were up-regulated by both stresses. In addition, 2742 and 1685 genes were down-regulated by drought and salt stresses, respectively, and 1564 genes were down-regulated by both stresses. The large number of genes regulated by both stresses suggests that crosstalk occurs between the drought and salt stress responses. Most up-regulated genes were involved in functions such as subcellular localization, signal transduction, metabolism, and transcription. Among the up-regulated genes, we identified 47 signaling proteins, 65 transcription factors, and 43 abiotic stress-related genes. Several genes were modulated by only one of the two stresses. About 25% of the genes significantly regulated by these stresses are of unknown function, suggesting that poplar may provide an opportunity to discover novel stress-related genes.
authors.affiliation: Department of Forest Genetic Resources, Korea Forest Research Institute, 39 Onjeong-ro, Suwon 441-847, Republic of Korea; Department of Forest Sciences, Seoul National University, 1 Gwanak-ro, Seoul 151-742, Republic of Korea.
authors.forename: Seo-Kyung
authors.initials: SK
authors.lastname: Yoon
authors.affiliation: Department of Forest Genetic Resources, Korea Forest Research Institute, 39 Onjeong-ro, Suwon 441-847, Republic of Korea.
authors.forename: Eung-Jun
authors.initials: EJ
authors.lastname: Park
authors.affiliation: Department of Forest Genetic Resources, Korea Forest Research Institute, 39 Onjeong-ro, Suwon 441-847, Republic of Korea.
authors.forename: Young-Im
authors.initials: YI
authors.lastname: Choi
authors.affiliation: Department of Forest Genetic Resources, Korea Forest Research Institute, 39 Onjeong-ro, Suwon 441-847, Republic of Korea.
authors.forename: Eun-Kyung
authors.initials: EK
authors.lastname: Bae
authors.affiliation: Department of Forest Genetic Resources, Korea Forest Research Institute, 39 Onjeong-ro, Suwon 441-847, Republic of Korea.
authors.forename: Joon-Hyeok
authors.initials: JH
authors.lastname: Kim
authors.affiliation: Department of Horticultural Science, Chungbuk National University, 52 Naesudong-ro, Cheongju 361-763, Republic of Korea.
authors.forename: So-Young
authors.initials: SY
authors.lastname: Park
authors.affiliation: Department of Forest Sciences, Seoul National University, 1 Gwanak-ro, Seoul 151-742, Republic of Korea.
authors.forename: Kyu-Suk
authors.initials: KS
authors.lastname: Kang
authors.affiliation: Department of Forest Genetic Resources, Korea Forest Research Institute, 39 Onjeong-ro, Suwon 441-847, Republic of Korea. Electronic address: hslee@forest.go.kr.
authors.forename: Hyoshin
authors.initials: H
authors.lastname: Lee
mesh_terms: D055864:Droughts; D018506:Gene Expression Regulation, Plant / Q000187:drug effects / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis; D018515:Plant Leaves / Q000187:drug effects* / Q000235:genetics*; D032107:Populus / Q000187:drug effects* / Q000235:genetics*; D012965:Sodium Chloride / Q000494:pharmacology*
chemical_list: D012965:Sodium Chloride
keywords: Drought stress; Gene expression; Oligonucleotide microarray; Populus; Salt stress