title: Derivation of GMP-compliant integration-free hiPSCs using modified mRNAs.
abstract: The clinical use of human induced pluripotent stem cells (hiPSCs) and the development of patients-specific gene and cell therapies rely on the development of fast, reliable, and integration-free methods of derivation of pluripotent stem cells from somatic tissues. Here we describe an integration-free protocol for the rapid derivation of hiPSCs from dermal fibroblasts using modified mRNAs. This method is inexpensive, highly efficient, and makes use of reagents that are xeno-free and chemically defined and can therefore be adopted by any Good Manufacturing Practice (GMP) facility.
authors.affiliation: Institute for Stem Cell Biology and Regenerative Medicine, Department of Obstetrics and Gynecology, Stanford University, Stanford, CA, 94305, USA.
authors.forename: Jens Durruthy
authors.initials: JD
authors.lastname: Durruthy
authors.forename: Vittorio
authors.initials: V
authors.lastname: Sebastiano
mesh_terms: D018929:Cell Culture Techniques / Q000379:methods* / Q000592:standards*; D002454:Cell Differentiation / Q000235:genetics*; D064987:Cell- and Tissue-Based Therapy / Q000379:methods / Q000592:standards; D065150:Cellular Reprogramming / Q000235:genetics*; D015925:Cryopreservation / Q000379:methods / Q000592:standards; D005347:Fibroblasts / Q000166:cytology; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology* / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics*; D014162:Transfection / Q000379:methods
chemical_list: D012333:RNA, Messenger