title: In vivoossification of a scaffold combining β-tricalcium phosphate and platelet-rich plasma.
abstract: Tricalcium phosphate (TCP) and platelet-rich plasma (PRP) are commonly used in bone tissue engineering. The aim of the present study was to investigate a composite that combined TCP with PRP and assess its effectiveness in the treatment of bone defects. Cavity-shaped bone defects were established on the tibiae of 27 beagle dogs, and were repaired by pure β-TCP with bone marrow stromal cells (BMSCs), β-TCP/PRP with BMSCs and autogenic ilium. The samples were harvested at 4, 8 and 12 weeks, and bone regeneration was evaluated using X-ray radiography, immunocytochemical staining of osteocalcin (OCN), hematoxylin and eosin staining and reverse transcription-polymerase chain reaction analyses. Biomechanical tests of the scaffolds were performed at the 12th week after scaffold implantation. When using pure β-TCP as a scaffold, the scaffold-bone interface was clear and no material adsorption and bone healing was observed. Substantial bone regeneration was observed when the tibial defects were restored using β-TCP/PRP and autogenic ilium. Furthermore, the mRNA expression levels of OCN, alkaline phosphatase and collagen type I α1 were significantly higher in the animals with β-TCP/PRP scaffolds at 8 and 12 weeks following implantation compared with those in the animals with the pure β-TCP scaffolds. The maximum load and compressive strength of the β-TCP/PRP scaffolds were similar to those of the autogenic ilium; however, they were significantly higher than those of the pure β-TCP scaffold. Thus, the β-TCP/PRP composite may be used as a potential scaffold to carryin vitrocultured BMSCs to treat bone defects.
authors.affiliation: Department of Orthopedics, Xiangya Hospital, Central South University, Changsha, Hunan 410008, P.R. China.
authors.forename: DA
authors.initials: D
authors.lastname: Zhong
authors.affiliation: Department of Orthopedics, Xiangya Hospital, Central South University, Changsha, Hunan 410008, P.R. China.
authors.forename: Cheng-Gong
authors.initials: CG
authors.lastname: Wang
authors.affiliation: Department of Orthopedics, Xiangya Hospital, Central South University, Changsha, Hunan 410008, P.R. China.
authors.forename: Ke
authors.initials: K
authors.lastname: Yin
authors.affiliation: Department of Orthopedics, Xiangya Hospital, Central South University, Changsha, Hunan 410008, P.R. China.
authors.forename: Qiande
authors.initials: Q
authors.lastname: Liao
authors.affiliation: Department of Orthopedics, Xiangya Hospital, Central South University, Changsha, Hunan 410008, P.R. China.
authors.forename: Xing
authors.initials: X
authors.lastname: Zhou
authors.affiliation: Department of Orthopedics, Xiangya Hospital, Central South University, Changsha, Hunan 410008, P.R. China.
authors.forename: An-Song
authors.initials: AS
authors.lastname: Liu
authors.affiliation: Department of Orthopedics, Xiangya Hospital, Central South University, Changsha, Hunan 410008, P.R. China.
authors.forename: Ling-Yu
authors.initials: LY
authors.lastname: Kong
keywords: bone defect; bone marrow stromal cells; platelet-rich plasma; tissue engineering; tricalcium phosphate