title: OXA-23 carbapenemase in multidrug-resistant Acinetobacter baumannii ST2 type: first identification in L'Aquila Hospital (Italy).
abstract: In this study 114 extensively drug-resistant Acinetobacter baumannii clinical isolates were characterized. The strains were collected at L'Aquila Hospital after the earthquake in L'Aquila city (central Italy) on the 6th of April 2009. The genes blaOXA-23 and blaOXA-51 were detected in all clinical isolates analyzed, whereas blaTEM-1 allele was detected in 56/114 isolates. The blaOXA-23 gene is located downstream the ISAba region and is under control of a strong promoter. On 42/80 A. baumannii the presence of two class 1 integrons was ascertained on chromosomal DNA. Variable regions show different gene array: (1) aadB and aadA2, (2) aacA4, aac(6')-Ib-cr, and aadA1. Macrorestriction analysis using ApaI restriction endonuclease identifies three clusters (A, B, and C) according to pulsed-field gel electrophoresis profiles. All isolates analyzed belong to the clone A. baumannii sequence type 2.
authors.affiliation: Dipartimento di Scienze Cliniche Applicate e Biotecnologiche, Università degli Studi dell'Aquila , L'Aquila, Italy .
authors.forename: Mariagrazia
authors.initials: M
authors.lastname: Perilli
authors.forename: Alessia
authors.initials: A
authors.lastname: Sabatini
authors.forename: Eugenio
authors.initials: E
authors.lastname: Pontieri
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Celenza
authors.forename: Bernardetta
authors.initials: B
authors.lastname: Segatore
authors.forename: Carlo
authors.initials: C
authors.lastname: Bottoni
authors.forename: Pierangelo
authors.initials: P
authors.lastname: Bellio
authors.forename: Alisia
authors.initials: A
authors.lastname: Mancini
authors.forename: Francesca
authors.initials: F
authors.lastname: Marcoccia
authors.forename: Fabrizia
authors.initials: F
authors.lastname: Brisdelli
authors.forename: Gianfranco
authors.initials: G
authors.lastname: Amicosante
mesh_terms: D000151:Acinetobacter Infections / Q000453:epidemiology / Q000382:microbiology*; D040981:Acinetobacter baumannii / Q000201:enzymology / Q000235:genetics / Q000302:isolation & purification*; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D017714:Community-Acquired Infections / Q000453:epidemiology / Q000382:microbiology; D003227:Conjugation, Genetic; D004269:DNA, Bacterial / Q000235:genetics; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics*; D016521:Electrophoresis, Gel, Pulsed-Field; D005798:Genes, Bacterial; D006784:Hospitals, Teaching / Q000706:statistics & numerical data; D006801:Humans; D007558:Italy / Q000453:epidemiology; D008969:Molecular Sequence Data; D058885:Multilocus Sequence Typing; D010957:Plasmids / Q000235:genetics; D012150:Polymorphism, Restriction Fragment Length; D001618:beta-Lactamases / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; C466679:beta-lactamase OXA-23; D001618:beta-Lactamases