title: Preventive supplementation with fresh and preserved peach attenuates CCl4-induced oxidative stress, inflammation and tissue damage.
abstract: The present study was elaborated to comparatively evaluate the preventive effect of different peach-derived products obtained from preserved fruits (Syrup and Preserve Pulp Peach [PPP]) and from fresh peels and pulps (Peel and Fresh Pulp Peach [FPP]) in a model of liver/renal toxicity and inflammation induced by carbon tetrachloride (CCl4) in rats. Tissue damage (carbonyl, thiobarbituric acid reactive species and sulfhydril), antioxidant enzymes activity (catalase and superoxide dismutase) and inflammatory parameters [tumor necrosis factor (TNF)-α and interleukin (IL)-1β levels, and receptor for advanced glycation end-products (RAGE) and nuclear factor (NF)κB-p65 immunocontent] were investigated. Our findings demonstrated that Peel, PPP and FPP (200 or 400 mg/kg) daily administration by oral gavage for 30 days conferred a significant protection against CCl4-induced antioxidant enzymes activation and, most importantly, oxidative damage to lipids and proteins as well as blocked induction of inflammatory mediators such as TNF-α, IL-1β, RAGE and NFκB. This antioxidant/anti-inflammatory effect seems to be associated with the abundance of carotenoids and polyphenols present in peach-derived products, which are enriched in fresh-fruit-derived preparations (Peel and FPP) but are also present in PPP. The Syrup - which was the least enriched in antioxidants - displayed no protective effect in our experiments. These effects cumulated in decreased levels of transaminases and lactate dehydrogenase leakage into serum and maintenance of organ architecture. Therefore, the herein presented results show evidence that supplementation with peach products may be protective against organ damage caused by oxidative stress, being interesting candidates for production of antioxidant-enriched functional foods.
authors.affiliation: Centro de Estudos em Estresse Oxidativo, Departamento de Bioquímica, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul (UFRGS) Brazil. Electronic address: Juciano.gasparotto@gmail.com.
authors.forename: Juciano
authors.initials: J
authors.lastname: Gasparotto
authors.affiliation: Centro de Estudos em Estresse Oxidativo, Departamento de Bioquímica, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul (UFRGS) Brazil.
authors.forename: Nauana
authors.initials: N
authors.lastname: Somensi
authors.affiliation: Centro de Estudos em Estresse Oxidativo, Departamento de Bioquímica, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul (UFRGS) Brazil.
authors.forename: Rafael Calixto
authors.initials: RC
authors.lastname: Bortolin
authors.affiliation: Centro de Estudos em Estresse Oxidativo, Departamento de Bioquímica, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul (UFRGS) Brazil.
authors.forename: Carolina Saibro
authors.initials: CS
authors.lastname: Girardi
authors.affiliation: Centro de Estudos em Estresse Oxidativo, Departamento de Bioquímica, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul (UFRGS) Brazil.
authors.forename: Alice
authors.initials: A
authors.lastname: Kunzler
authors.affiliation: Centro de Estudos em Estresse Oxidativo, Departamento de Bioquímica, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul (UFRGS) Brazil.
authors.forename: Thallita Kelly
authors.initials: TK
authors.lastname: Rabelo
authors.affiliation: Centro de Estudos em Estresse Oxidativo, Departamento de Bioquímica, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul (UFRGS) Brazil.
authors.forename: Carlos Eduardo
authors.initials: CE
authors.lastname: Schnorr
authors.affiliation: Centro de Estudos em Estresse Oxidativo, Departamento de Bioquímica, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul (UFRGS) Brazil.
authors.forename: Karla Suzana
authors.initials: KS
authors.lastname: Moresco
authors.affiliation: Programa de Pós-Graduação em Ciências Farmacêuticas, Faculdade de Farmácia, Universidade Federal Rio Grande do Sul (UFRGS) Brazil.
authors.forename: Valquiria Linck
authors.initials: VL
authors.lastname: Bassani
authors.affiliation: Programa de Pós-Graduação em Ciências Farmacêuticas, Faculdade de Farmácia, Universidade Federal Rio Grande do Sul (UFRGS) Brazil.
authors.forename: Francini Kiyono Jorge
authors.initials: FK
authors.lastname: Yatsu
authors.affiliation: Embrapa Clima Temperado, Empresa Brasileira de Pesquisa Agropecuária, Pelotas/RS Brazil.
authors.forename: Márcia
authors.initials: M
authors.lastname: Vizzotto
authors.affiliation: Embrapa Clima Temperado, Empresa Brasileira de Pesquisa Agropecuária, Pelotas/RS Brazil.
authors.forename: Maria do Carmo Bassols
authors.initials: Mdo C
authors.lastname: Raseira
authors.affiliation: Centro de Estudos em Estresse Oxidativo, Departamento de Bioquímica, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul (UFRGS) Brazil.
authors.forename: Alfeu
authors.initials: A
authors.lastname: Zanotto-Filho
authors.affiliation: Centro de Estudos em Estresse Oxidativo, Departamento de Bioquímica, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul (UFRGS) Brazil.
authors.forename: José Claudio Fonseca
authors.initials: JC
authors.lastname: Moreira
authors.affiliation: Centro de Estudos em Estresse Oxidativo, Departamento de Bioquímica, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul (UFRGS) Brazil.
authors.forename: Daniel Pens
authors.initials: DP
authors.lastname: Gelain
mesh_terms: D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D001219:Aspartate Aminotransferases / Q000097:blood; D001663:Bilirubin / Q000097:blood; D001786:Blood Glucose / Q000378:metabolism; D002251:Carbon Tetrachloride / Q000009:adverse effects*; D002338:Carotenoids / Q000032:analysis; D019587:Dietary Supplements; D005638:Fruit / Q000737:chemistry*; D007249:Inflammation / Q000139:chemically induced / Q000188:drug therapy; D053583:Interleukin-1beta / Q000097:blood; D007668:Kidney / Q000187:drug effects / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008297:Male; D016328:NF-kappa B / Q000097:blood; D018384:Oxidative Stress / Q000187:drug effects*; D008517:Phytotherapy / Q000379:methods; D028321:Plant Preparations / Q000494:pharmacology*; D059808:Polyphenols / Q000032:analysis; D027861:Prunus / Q000737:chemistry*; D051381:Rats; D017208:Rats, Wistar; D000067759:Receptor for Advanced Glycation End Products; D011971:Receptors, Immunologic / Q000097:blood; D013482:Superoxide Dismutase / Q000097:blood; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000097:blood
chemical_list: D000975:Antioxidants; D001786:Blood Glucose; D053583:Interleukin-1beta; D016328:NF-kappa B; D028321:Plant Preparations; D059808:Polyphenols; D000067759:Receptor for Advanced Glycation End Products; D011971:Receptors, Immunologic; D017392:Thiobarbituric Acid Reactive Substances; D014409:Tumor Necrosis Factor-alpha; D002338:Carotenoids; D002251:Carbon Tetrachloride; D013482:Superoxide Dismutase; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D001663:Bilirubin
keywords: Anti-inflammatory; Antioxidant; Carbon tetrachloride; NFκB-p65; Peach; RAGE