title: Evaluation of biochemical and molecular methods for Lactobacillus reuteri strains differentiation.
abstract: Several biochemical and molecular methods were used for discrimination of four Lactobacillus reuteri strains isolated from goatling and lamb stomach mucosa. Internal transcribed spacer (ITS)-PCR method and protein analysis by SDS-PAGE and MALDI-TOF showed to be suitable for strain discrimination whereas ITS-PCR/RFLP and enterobacterial repetitive intergenic consensus (ERIC)-PCR were not strain specific. The used methods differentiated tested strains into distinct groups; however, the location of strains in groups varied. Consistency in results was observed in the case of L. reuteri E and L. reuteri KO4m that were clustered into the same groups using all techniques, except of MALDI-TOF MS. The last one grouped goatling strains and lamb isolate into separate clusters. All investigated methods, except of ITS-PCR/RFLP and ERIC-PCR, were assessed as appropriate for distinguishing of L. reuteri strains.
authors.affiliation: Department of Cell and Molecular Biology of Drugs, Faculty of Pharmacy, Comenius University in Bratislava, Bratislava, Slovak Republic, bilkova@fpharm.uniba.sk.
authors.forename: Bilková
authors.initials: B
authors.lastname: Andrea
authors.forename: Kiňová Sepová
authors.initials: KS
authors.lastname: Hana
authors.forename: Dubničková
authors.initials: D
authors.lastname: Martina
authors.forename: Májeková
authors.initials: M
authors.lastname: Hyacinta
authors.forename: Bilka
authors.initials: B
authors.lastname: František
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000032:analysis; D001431:Bacteriological Techniques / Q000379:methods*; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000737:chemistry / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D005753:Gastric Mucosa / Q000382:microbiology; D006041:Goats; D052200:Limosilactobacillus reuteri / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology; D016133:Polymerase Chain Reaction; D015203:Reproducibility of Results; D012756:Sheep; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D021903:DNA, Ribosomal Spacer