title: Antioxidants in the prevention of UVB-induced keratynocyte apoptosis.
abstract: Skin cells can respond to UVB-induced damage by counteracting it through antioxidant activation and DNA repair mechanisms or, when damage is massive by undergoing programmed cell death. Antioxidant factors, and, in particular, food compounds, have attracted much interest because of their potential use in new protective strategies for degenerative skin disorders. Melatonin, creatine and hydroxytyrosol show a variety of pharmacological and clinical benefits including anti-oxidant and anti-inflammatory activities. Here, the potential protective actions of antioxidant compounds against UVB-induced apoptosis were investigated in human keratinocytes. The cells were pre-treated with antioxidants before UVB exposure and their effect evaluated by means of ultrastructural and molecular analyses. After UVB radiation typical morphological apoptotic features and in situ DNA fragmentation after TUNEL reaction, appeared. A significant numerical decrease of apoptotic patterns could be observed when antioxidants were administrated before cell death induction. Moreover, both the intrinsic and extrinsic apoptotic pathways appeared activated after UVB radiation, and their down-regulation has been shown when antioxidants were added to cells before death induction. In conclusion, these compounds are able to prevent apoptotic cell death in human keratinocytes exposed to UVB, suggesting, for these molecules, an important role in preventing skin damage.
authors.affiliation: DiSTeVA, University of Urbino Carlo Bo, Urbino 61029, Italy.
authors.forename: Sara
authors.initials: S
authors.lastname: Salucci
authors.affiliation: DiSTeVA, University of Urbino Carlo Bo, Urbino 61029, Italy.
authors.forename: Sabrina
authors.initials: S
authors.lastname: Burattini
authors.affiliation: DiSTeVA, University of Urbino Carlo Bo, Urbino 61029, Italy.
authors.forename: Davide
authors.initials: D
authors.lastname: Curzi
authors.affiliation: Department of Biomedical and Neuromotor Sciences, University of Bologna, Bologna, Italy.
authors.forename: Francesca
authors.initials: F
authors.lastname: Buontempo
authors.affiliation: Department of Biomedical and Neuromotor Sciences, University of Bologna, Bologna, Italy.
authors.forename: Alberto Maria
authors.initials: AM
authors.lastname: Martelli
authors.affiliation: DiSB, University of Urbino Carlo Bo, Urbino 61029, Italy.
authors.forename: Giovanni
authors.initials: G
authors.lastname: Zappia
authors.affiliation: DiSTeVA, University of Urbino Carlo Bo, Urbino 61029, Italy; IGM, CNR, Rizzoli Orthopaedic Institute, Bologna 40136, Italy.
authors.forename: Elisabetta
authors.initials: E
authors.lastname: Falcieri
authors.affiliation: DiSTeVA, University of Urbino Carlo Bo, Urbino 61029, Italy.
authors.forename: Michela
authors.initials: M
authors.lastname: Battistelli
mesh_terms: D000975:Antioxidants / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects* / Q000528:radiation effects; D020169:Caspases / Q000378:metabolism; D002460:Cell Line; D003401:Creatine / Q000494:pharmacology; D053938:DNA Fragmentation / Q000187:drug effects / Q000528:radiation effects; D006801:Humans; D007365:Intercellular Junctions / Q000648:ultrastructure; D015603:Keratinocytes / Q000166:cytology / Q000378:metabolism; D008550:Melatonin / Q000494:pharmacology; D008856:Microscopy, Fluorescence; D010626:Phenylethyl Alcohol / Q000031:analogs & derivatives / Q000494:pharmacology; D011065:Poly(ADP-ribose) Polymerases / Q000378:metabolism; D014466:Ultraviolet Rays
chemical_list: D000975:Antioxidants; C005975:3,4-dihydroxyphenylethanol; D011065:Poly(ADP-ribose) Polymerases; D020169:Caspases; D008550:Melatonin; D010626:Phenylethyl Alcohol; D003401:Creatine
keywords: Antioxidants; Apoptosis; Caspases; HaCaT; UVB; Ultrastructure