title: Trafficking and function of GPCRs in the endosomal compartment.
abstract: New methods based on fluorescently labeled agonists, genetically encoded fluorescent sensors, and advanced microscopy techniques, such as fluorescence resonance energy transfer (FRET) and highly inclined thin illumination (HILO), allow direct monitoring of signaling, internalization, and intracellular trafficking of G protein-coupled receptors (GPCRs) and their ligands in living cells with high temporal and spatial resolution. These methods have been essential in revealing that GPCRs can continue signaling via production of the soluble second messenger cyclic AMP after internalization into the endosomal compartment.
authors.affiliation: Bio-Imaging Center/Rudolf Virchow Center for Experimental Biomedicine, and Institute of Pharmacology and Toxicology, University of Würzburg, Versbacher Strasse 9, 97078, Würzburg, Germany, davide.calebiro@toxi.uni-wuerzburg.de.
authors.forename: Davide
authors.initials: D
authors.lastname: Calebiro
authors.forename: Amod
authors.initials: A
authors.lastname: Godbole
authors.forename: Sandra
authors.initials: S
authors.lastname: Lyga
authors.forename: Martin J
authors.initials: MJ
authors.lastname: Lohse
mesh_terms: D000818:Animals; D000242:Cyclic AMP / Q000378:metabolism; D011992:Endosomes / Q000378:metabolism*; D031541:Fluorescence Resonance Energy Transfer; D008024:Ligands; D051379:Mice; D008856:Microscopy, Fluorescence / Q000379:methods; D057054:Molecular Imaging / Q000379:methods*; D061251:Primary Cell Culture; D021381:Protein Transport; D043562:Receptors, G-Protein-Coupled / Q000378:metabolism*; D015398:Signal Transduction; D013961:Thyroid Gland / Q000166:cytology
chemical_list: D008024:Ligands; D043562:Receptors, G-Protein-Coupled; D000242:Cyclic AMP