title: TaSK5, an abiotic stress-inducible GSK3/shaggy-like kinase from wheat, confers salt and drought tolerance in transgenic Arabidopsis.
abstract: A novel cold-inducible GSK3/shaggy-like kinase, TaSK5, was isolated from winter wheat using a macroarray-based differential screening approach. TaSK5 showed high similarity to Arabidopsis subgroup I GSK3/shaggy-like kinases ASK-alpha, AtSK-gamma and ASK-epsilon. RNA gel blot analyses revealed TaSK5 induction by cold and NaCl treatments and to a lesser extent by drought treatment. TaSK5 functionally complemented the cold- and salt-sensitive phenotypes of a yeast GSK3/shaggy-like kinase mutant, △mck1. Transgenic Arabidopsis plants overexpressing TaSK5 cDNA showed enhanced tolerance to salt and drought stresses. By contrast, the tolerance of the transgenic plants to freezing stress was not altered. Microarray analysis revealed that a number of abiotic stress-inducible genes were constitutively induced in the transgenic Arabidopsis plants, suggesting that TaSK5 may function in a novel signal transduction pathway that appears to be unrelated to DREB1/CBF regulon and may involve crosstalk between abiotic and hormonal signals.
authors.affiliation: Hokkaido Agricultural Research Center, National Agriculture and Food Research Organization (NARO), Hitsujigaoka 1, Toyohira-ku, Sapporo 062-8555, Japan; AgroBioInstitute, Dragan Tsankov 8, Sofia 1164, Bulgaria.
authors.forename: Nikolai Kirilov
authors.initials: NK
authors.lastname: Christov
authors.affiliation: Hokkaido Agricultural Research Center, National Agriculture and Food Research Organization (NARO), Hitsujigaoka 1, Toyohira-ku, Sapporo 062-8555, Japan; AgroBioInstitute, Dragan Tsankov 8, Sofia 1164, Bulgaria.
authors.forename: Petya Koeva
authors.initials: PK
authors.lastname: Christova
authors.affiliation: Hokkaido Agricultural Research Center, National Agriculture and Food Research Organization (NARO), Hitsujigaoka 1, Toyohira-ku, Sapporo 062-8555, Japan.
authors.forename: Hideki
authors.initials: H
authors.lastname: Kato
authors.affiliation: Hokkaido Agricultural Research Center, National Agriculture and Food Research Organization (NARO), Hitsujigaoka 1, Toyohira-ku, Sapporo 062-8555, Japan; Graduate School of Agriculture, Hokkaido University, Sapporo 060-8589, Japan.
authors.forename: Yuelin
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Hokkaido Agricultural Research Center, National Agriculture and Food Research Organization (NARO), Hitsujigaoka 1, Toyohira-ku, Sapporo 062-8555, Japan.
authors.forename: Kentaro
authors.initials: K
authors.lastname: Sasaki
authors.affiliation: Hokkaido Agricultural Research Center, National Agriculture and Food Research Organization (NARO), Hitsujigaoka 1, Toyohira-ku, Sapporo 062-8555, Japan; Graduate School of Agriculture, Hokkaido University, Sapporo 060-8589, Japan. Electronic address: rzi@affrc.go.jp.
authors.forename: Ryozo
authors.initials: R
authors.lastname: Imai
mesh_terms: D017360:Arabidopsis / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism; D055864:Droughts; D018506:Gene Expression Regulation, Plant / Q000187:drug effects / Q000235:genetics; D030821:Plants, Genetically Modified / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism*; D012965:Sodium Chloride / Q000494:pharmacology*; D014908:Triticum / Q000201:enzymology* / Q000235:genetics
chemical_list: D029681:Arabidopsis Proteins; D012965:Sodium Chloride
keywords: Arabidopsis; Cold; Drought; Salt; Signal transduction; Triticum