title: Isolation of Aureimonas altamirensis, a Brucella canis-like bacterium, from an edematous canine testicle.
abstract: Microbiological and histological analysis of a sample from a swollen testicle of a 2-year-old Border Collie dog revealed a mixed infection of the fungus Blastomyces dermatitidis and the Gram-negative bacterium Aureimonas altamirensis. When subjected to an automated microbial identification system, the latter isolate was provisionally identified as Psychrobacter phenylpyruvicus, but the organism shared several biochemical features with Brucella canis and exhibited agglutination, albeit weakly, with anti-B. canis antiserum. Unequivocal identification of the organism was only achieved by 16S ribosomal RNA gene sequencing, ultimately establishing the identity as A. altamirensis. Since its first description in 2006, this organism has been isolated infrequently from human clinical samples, but, to the authors' knowledge, has not been reported from a veterinary clinical sample. While of unknown clinical significance with respect to the pathology observed for the polymicrobial infection described herein, it highlights the critical importance to unambiguously identify the microbe for diagnostic, epidemiological, infection control, and public health purposes.
authors.affiliation: Veterinary Medical Diagnostic Laboratory (Reilly, Wennerdahl, Williams, Evans, Ganjam, Bowman, Fales), University of Missouri, Columbia, MODepartment of Veterinary Pathobiology (Reilly, Calcutt, Wennerdahl, Williams, Evans, Ganjam, Bowman, Fales), University of Missouri, Columbia, MO reillyt@missouri.edu.
authors.forename: Thomas J
authors.initials: TJ
authors.lastname: Reilly
authors.affiliation: Veterinary Medical Diagnostic Laboratory (Reilly, Wennerdahl, Williams, Evans, Ganjam, Bowman, Fales), University of Missouri, Columbia, MODepartment of Veterinary Pathobiology (Reilly, Calcutt, Wennerdahl, Williams, Evans, Ganjam, Bowman, Fales), University of Missouri, Columbia, MO.
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Calcutt
authors.affiliation: Veterinary Medical Diagnostic Laboratory (Reilly, Wennerdahl, Williams, Evans, Ganjam, Bowman, Fales), University of Missouri, Columbia, MODepartment of Veterinary Pathobiology (Reilly, Calcutt, Wennerdahl, Williams, Evans, Ganjam, Bowman, Fales), University of Missouri, Columbia, MO.
authors.forename: Laura A
authors.initials: LA
authors.lastname: Wennerdahl
authors.affiliation: Veterinary Medical Diagnostic Laboratory (Reilly, Wennerdahl, Williams, Evans, Ganjam, Bowman, Fales), University of Missouri, Columbia, MODepartment of Veterinary Pathobiology (Reilly, Calcutt, Wennerdahl, Williams, Evans, Ganjam, Bowman, Fales), University of Missouri, Columbia, MO.
authors.forename: Fred
authors.initials: F
authors.lastname: Williams
authors.affiliation: Veterinary Medical Diagnostic Laboratory (Reilly, Wennerdahl, Williams, Evans, Ganjam, Bowman, Fales), University of Missouri, Columbia, MODepartment of Veterinary Pathobiology (Reilly, Calcutt, Wennerdahl, Williams, Evans, Ganjam, Bowman, Fales), University of Missouri, Columbia, MO.
authors.forename: Tim J
authors.initials: TJ
authors.lastname: Evans
authors.affiliation: Veterinary Medical Diagnostic Laboratory (Reilly, Wennerdahl, Williams, Evans, Ganjam, Bowman, Fales), University of Missouri, Columbia, MODepartment of Veterinary Pathobiology (Reilly, Calcutt, Wennerdahl, Williams, Evans, Ganjam, Bowman, Fales), University of Missouri, Columbia, MO.
authors.forename: Irene K
authors.initials: IK
authors.lastname: Ganjam
authors.affiliation: Veterinary Medical Diagnostic Laboratory (Reilly, Wennerdahl, Williams, Evans, Ganjam, Bowman, Fales), University of Missouri, Columbia, MODepartment of Veterinary Pathobiology (Reilly, Calcutt, Wennerdahl, Williams, Evans, Ganjam, Bowman, Fales), University of Missouri, Columbia, MO.
authors.forename: Jesse W
authors.initials: JW
authors.lastname: Bowman
authors.affiliation: Veterinary Medical Diagnostic Laboratory (Reilly, Wennerdahl, Williams, Evans, Ganjam, Bowman, Fales), University of Missouri, Columbia, MODepartment of Veterinary Pathobiology (Reilly, Calcutt, Wennerdahl, Williams, Evans, Ganjam, Bowman, Fales), University of Missouri, Columbia, MO.
authors.forename: William H
authors.initials: WH
authors.lastname: Fales
mesh_terms: D020561:Alphaproteobacteria / Q000235:genetics / Q000302:isolation & purification*; D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D004269:DNA, Bacterial / Q000235:genetics / Q000378:metabolism; D004283:Dog Diseases / Q000175:diagnosis* / Q000382:microbiology*; D004285:Dogs; D004487:Edema / Q000175:diagnosis / Q000382:microbiology / Q000662:veterinary*; D016905:Gram-Negative Bacterial Infections / Q000175:diagnosis / Q000382:microbiology / Q000662:veterinary*; D008297:Male; D008969:Molecular Sequence Data; D012336:RNA, Ribosomal, 16S / Q000235:genetics / Q000378:metabolism; D017422:Sequence Analysis, DNA / Q000662:veterinary; D013733:Testicular Diseases / Q000175:diagnosis / Q000382:microbiology / Q000662:veterinary*; D016896:Treatment Outcome
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S
keywords: Aureimonas altamirensis; Blastomyces dermatitidis; Brucella canis; canine testicle