title: Classification and characterization of a laboratory chicken rotavirus strain carrying G7P[35] neutralization antigens on the genotype 4 backbone gene configuration.
abstract: The laboratory rotavirus strain, BRS/115, has been used for more than two decades to monitor rotaviruses in specific pathogen free flocks of laying hens. However, the virus strain has not been characterized in detail. Therefore we aimed at the description of molecular features of BRS115 by using random primed reverse transcription-PCR of the genomic RNA followed by massive parallel sequencing using the semiconductor sequencing technology. Over 64,000 trimmed reads mapped to reference sequences obtained from GenBank. The strain classified into the species Rotavirus A and genotyped G7-P[35]-I4-R4-C4-M4-A16-N4-T4-E11-H4 according to guidelines of the Rotavirus Classification Working Group. Phylogenetic analysis identified shared features with chicken, turkey and pigeon origin rotaviruses. This study demonstrates the robustness of next generation sequencing in the characterization of reference virus materials used in specialized laboratories.
authors.affiliation: Institute for Veterinary Medical Research, Centre for Agricultural Research, Hungarian Academy of Sciences, Hungária krt. 21, H-1143 Budapest, Hungary.
authors.forename: Hajnalka
authors.initials: H
authors.lastname: Papp
authors.affiliation: Institute for Veterinary Medical Research, Centre for Agricultural Research, Hungarian Academy of Sciences, Hungária krt. 21, H-1143 Budapest, Hungary.
authors.forename: Szilvia
authors.initials: S
authors.lastname: Marton
authors.affiliation: Institute for Veterinary Medical Research, Centre for Agricultural Research, Hungarian Academy of Sciences, Hungária krt. 21, H-1143 Budapest, Hungary.
authors.forename: Szilvia L
authors.initials: SL
authors.lastname: Farkas
authors.affiliation: Institute of Biology, Faculty of Sciences, University of Pécs, Ifjúság útja 6., H-7624 Pécs, Hungary; János Szentágothai Research Center, University of Pécs, Ifjúság útja 20., H-7624 Pécs, Hungary.
authors.forename: Ferenc
authors.initials: F
authors.lastname: Jakab
authors.affiliation: Department of Veterinary Public Health, University of Bari, S.p. per Casamassima km 3, 70010 Valenzano, Bari, Italy.
authors.forename: Vito
authors.initials: V
authors.lastname: Martella
authors.affiliation: Division of Biological Standardisation, Indian Veterinary Research Institute (IVRI), Izatnagar, 243 122 Bareilly, Uttar Pradesh, India.
authors.forename: Yashpal S
authors.initials: YS
authors.lastname: Malik
authors.affiliation: Ceva-Phylaxia Veterinary Biologicals Co. Ltd., Szállás u. 5., H-1107 Budapest, Hungary.
authors.forename: Vilmos
authors.initials: V
authors.lastname: Palya
authors.affiliation: Institute for Veterinary Medical Research, Centre for Agricultural Research, Hungarian Academy of Sciences, Hungária krt. 21, H-1143 Budapest, Hungary. Electronic address: bkrota@hotmail.com.
authors.forename: Krisztián
authors.initials: K
authors.lastname: Bányai
mesh_terms: D000818:Animals; D002645:Chickens / Q000821:virology*; D018076:DNA, Complementary / Q000378:metabolism; D015723:Gene Library; D016679:Genome, Viral; D005838:Genotype; D059014:High-Throughput Nucleotide Sequencing; D010802:Phylogeny; D012313:RNA / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012401:Rotavirus / Q000235:genetics; D012666:Semiconductors; D017422:Sequence Analysis, DNA
chemical_list: D018076:DNA, Complementary; D012313:RNA
keywords: Genotype; Phylogeny; Rotavirus A