title: Effect of glutathione and Y27632 on the viability of cryopreserved porcine adipose-derived stem cells.
abstract: BACKGROUND
  Recently it has been reported that reduced glutathione (GSH) and/or Rho-associated kinase (ROCK) inhibitor supplemented in cryopreservation solution could improve the viability of cells.
  
  
  OBJECTIVE
  To identify the cryopreservation efficiency of GSH and ROCK inhibitor on porcine ADSCs.
  
  
  METHODS
  Porcine ADSCs were separated and cultured. Cells at the 4th passage were suspended in cryopreservation solution supplemented with Y-27632 and GSH or both, and then frozen and thawed. The viability of cryopreserved ADSCs was compared using a MTT assay.
  
  
  RESULTS
  The addition of GSH and Y-27632 to cryopreservation solution (dimethyl sulfoxide) and post-thaw culture medium significantly improves the post-thaw viability of ADSCs.
  
  
  CONCLUSIONS
  GSH and Y-27632 are able to increase the survival of ADSCs, and they have an additive effect, as compared to GSH or Y27632 alone.
authors.affiliation: School of Life Science, FuYang Teachers College, Fuyang, Anhui, China. qucq518@163.com.
authors.forename: C-Q
authors.initials: CQ
authors.lastname: Qu
authors.affiliation: School of Life Science, FuYang Teachers College, Fuyang, Anhui, China.
authors.forename: D-W
authors.initials: DW
authors.lastname: Li
authors.affiliation: School of Life Science, FuYang Teachers College, Fuyang, Anhui, China.
authors.forename: N
authors.initials: N
authors.lastname: Shen
authors.affiliation: School of Life Science, FuYang Teachers College, Fuyang, Anhui, China.
authors.forename: J-H
authors.initials: JH
authors.lastname: Jiang-Yang
authors.affiliation: School of Life Science, FuYang Teachers College, Fuyang, Anhui, China.
authors.forename: Y-T
authors.initials: YT
authors.lastname: Ji
mesh_terms: D000273:Adipose Tissue / Q000166:cytology / Q000502:physiology; D000577:Amides / Q000494:pharmacology*; D000818:Animals; D002469:Cell Separation; D002470:Cell Survival / Q000187:drug effects; D015925:Cryopreservation / Q000379:methods*; D003451:Cryoprotective Agents / Q000494:pharmacology*; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D005978:Glutathione / Q000494:pharmacology*; D061251:Primary Cell Culture; D011725:Pyridines / Q000494:pharmacology*; D013234:Stem Cells / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D013552:Swine
chemical_list: D000577:Amides; D003451:Cryoprotective Agents; D004791:Enzyme Inhibitors; D011725:Pyridines; C108830:Y 27632; D005978:Glutathione; D004121:Dimethyl Sulfoxide