title: Genome-wide analysis of methylation in bovine clones by methylated DNA immunoprecipitation (MeDIP).
abstract: Methylated DNA immunoprecipitation (MeDIP), when coupled to high-throughput sequencing or microarray hybridization, allows for the identification of methylated loci at a genome-wide scale. Genomic regions affected by incomplete reprogramming after nuclear transfer can potentially be delineated by comparing the MeDIP profiles of bovine clones and non-clones. This chapter presents a MeDIP protocol largely inspired from Mohn and colleagues (Mohn et al., Methods Mol Biol 507:55-64, 2009), with PCR primers specific for cattle, and when possible, overviews of experimental designs adapted to the comparison between clones and non-clones.
authors.affiliation: INRA, UMR 1198 Biologie du Développement et Reproduction, Jouy-en-Josas, 78350, France, helene.kiefer@jouy.inra.fr.
authors.forename: Hélène
authors.initials: H
authors.lastname: Kiefer
mesh_terms: D000818:Animals; D002417:Cattle; D019976:Cloning, Organism; D018899:CpG Islands; D004247:DNA / Q000302:isolation & purification; D019175:DNA Methylation; D055106:Genome-Wide Association Study; D047468:Immunoprecipitation / Q000295:instrumentation / Q000379:methods*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D011786:Quality Control; D013010:Sonication
chemical_list: D004247:DNA