title: Chelation of trace elements in preservation medium influences the quality of boar spermatozoa during liquid preservation at 5°C for 4 weeks.
abstract: BACKGROUND
  The addition of a metal chelator, ethylenediaminetetraacetic acid (EDTA), to semen extender has the purpose of capturing trace element ions.
  
  
  OBJECTIVE
  This study was conducted to evaluate the effects of EDTA on the quality and in vitro fertilisability of liquid-preserved boar spermatozoa.
  
  
  METHODS
  In Experiment 1, semen samples were preserved in the semen extender supplemented with 0, 3, 6, or 12 mM of Na-EDTA at 5 degree C for 4 weeks. In Experiment 2, semen samples were preserved in the extender supplemented with 3 mM of Na-EDTA, Ca-EDTA, or Zn-EDTA and without chelator EDTA.
  
  
  RESULTS
  When Na-EDTA was used as a chelating substance in the extender, 3 mM was a most suitable concentration for sperm motility and viability after cold preservation. The supplementation of 3 mM Ca-EDTA had advantages regarding sperm motility, viability and plasma membrane integrity.
  
  
  CONCLUSIONS
  Our findings indicate that 3 mM Ca-EDTA is the most suitable metal-chelating substance for the liquid preservation of boar semen.
authors.affiliation: Laboratory of Animal Reproduction, The United Graduate School of Veterinary Science, Yamaguchi University, Yamaguchi, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Taniguchi
authors.affiliation: Department of Obstetrics, Gynaecology and Reproduction, Faculty of Veterinary Sciences, Chulalongkorn University, Bangkok, Thailand.
authors.forename: M
authors.initials: M
authors.lastname: Wittayarat
authors.affiliation: Laboratory of Animal Reproduction, The United Graduate School of Veterinary Science, Yamaguchi University, Yamaguchi, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Morinaga
authors.affiliation: Laboratory of Animal Reproduction, The United Graduate School of Veterinary Science, Yamaguchi University, Yamaguchi, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Sato
authors.affiliation: Laboratory of Animal Reproduction, The United Graduate School of Veterinary Science, Yamaguchi University, Yamaguchi, Japan.
authors.forename: L T
authors.initials: LT
authors.lastname: Kin Do
authors.affiliation: Department of Obstetrics, Gynaecology and Reproduction, Faculty of Veterinary Sciences, Chulalongkorn University, Bangkok, Thailand.
authors.forename: K
authors.initials: K
authors.lastname: Chatdarong
authors.affiliation: Department of Obstetrics, Gynaecology and Reproduction, Faculty of Veterinary Sciences, Chulalongkorn University, Bangkok, ThailandM.
authors.forename: M
authors.initials: M
authors.lastname: Techakumphu
authors.affiliation: Tokushima Prefectural Livestock Research Institute, Tokushima, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Nii
authors.affiliation: Laboratory of Animal Reproduction, The United Graduate School of Veterinary Science, Yamaguchi University, Yamaguchi, Japan. otoi@yamaguchi-u.ac.jp.
authors.forename: T
authors.initials: T
authors.lastname: Otoi
mesh_terms: D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D002614:Chelating Agents / Q000494:pharmacology*; D003470:Culture Media / Q000737:chemistry; D004492:Edetic Acid / Q000494:pharmacology*; D005307:Fertilization in Vitro; D008297:Male; D009865:Oocytes / Q000166:cytology / Q000254:growth & development; D020011:Protective Agents / Q000494:pharmacology*; D012034:Refrigeration; D012662:Semen Preservation / Q000379:methods*; D013081:Sperm Motility / Q000187:drug effects; D013094:Spermatozoa / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D013552:Swine; D013997:Time Factors
chemical_list: D002614:Chelating Agents; D003470:Culture Media; D020011:Protective Agents; D004492:Edetic Acid