title: First report on the antibody verification of HLA-DR, HLA-DQ and HLA-DP epitopes recorded in the HLA Epitope Registry.
abstract: The International Registry of Antibody-Defined HLA Epitopes (http://www.epregistry.com.br) has been recently established as a tool to understand humoral responses to HLA mismatches. These epitopes can be structurally defined as eplets by three-dimensional molecular modeling and amino acid sequence differences between HLA antigens. A major goal is to identify HLA eplets that have been verified experimentally with informative antibodies. This report addresses class II epitopes encoded by genes in the HLA-D region. Our analysis included reviews of many publications about epitope specificity of class II reactive human and murine monoclonal antibodies and informative alloantibodies from HLA sensitized patients as well as our own antibody testing results. As of July 1, 2014, 24 HLA-DRB1/3/4/5, 15 DQB, 3 DQA and 8 DPB antibody-verified epitopes have been identified and recorded. The Registry is still a work-in-progress and will become a useful resource for HLA professionals interested in histocompatibility testing at the epitope level and investigating antibody responses to HLA mismatches in transplant patients.
authors.affiliation: Division of Transplant Pathology, University of Pittsburgh Medical Center, USA. Electronic address: Duquesnoyr@upmc.edu.
authors.forename: Rene J
authors.initials: RJ
authors.lastname: Duquesnoy
authors.affiliation: Division of Transplant Pathology, University of Pittsburgh Medical Center, USA.
authors.forename: Marilyn
authors.initials: M
authors.lastname: Marrari
authors.affiliation: Transplant Immunology Laboratory, Feinberg School of Medicine, Northwestern University, Chicago, IL, USA.
authors.forename: Anat R
authors.initials: AR
authors.lastname: Tambur
authors.affiliation: Department of Immunohematology, Leiden University Medical Center, The Netherlands.
authors.forename: Arend
authors.initials: A
authors.lastname: Mulder
authors.affiliation: Department of Informatics and Statistics, Federal University of Piauí, Teresina, Brazil.
authors.forename: Luiz Cláudio Demes da Mata
authors.initials: LC
authors.lastname: Sousa
authors.affiliation: Department of Informatics and Statistics, Federal University of Piauí, Teresina, Brazil.
authors.forename: Adalberto Socorro
authors.initials: AS
authors.lastname: da Silva
authors.affiliation: Immunogenetics and Molecular Biology Laboratory, Federal University of Piauí, Teresina, Brazil.
authors.forename: Semiramis J H
authors.initials: SJ
authors.lastname: do Monte
mesh_terms: D000569:Americas; D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry; D000939:Epitopes / Q000737:chemistry / Q000276:immunology*; D005060:Europe; D006682:HLA-DP Antigens / Q000737:chemistry / Q000276:immunology*; D006683:HLA-DQ Antigens / Q000737:chemistry / Q000276:immunology*; D006684:HLA-DR Antigens / Q000737:chemistry / Q000276:immunology*; D006650:Histocompatibility Testing; D006801:Humans; D007391:International Cooperation; D007518:Isoantibodies / Q000737:chemistry; D051379:Mice; D012042:Registries
chemical_list: D000911:Antibodies, Monoclonal; D000939:Epitopes; D006682:HLA-DP Antigens; D006683:HLA-DQ Antigens; D006684:HLA-DR Antigens; D007518:Isoantibodies
keywords: HLA epitopes; HLA-DP epitopes; HLA-DQ epitopes; HLA-DRB epitopes