title: Lateral-flow assay for rapid quantitative detection of clorprenaline residue in swine urine.
abstract: Clorprenaline (CLP), a β2-adrenergic agonist, was first found in veterinary drugs for cold treatment in China in 2013. It is a potential new lean meat-boosting feed additive because it can promote animal muscular mass growth and decrease fat accumulation. A competitive colloidal gold-based lateral flow immunoassay system with a portable strip reader was successfully developed for rapid quantitative detection of CLP residue in swine urine. The detection system was optimized so that the detection can be completed within 9 min with a limit of detection of 0.15 μg · liter(-1). The assay exhibited good linear range from 3.0 to 20.0 μg · liter(-1), with reliable correlation of 0.9970 and with no obvious cross-reaction with five other β2-agonist compounds. Twenty spiked swine urine samples were tested by lateral flow immunoassay and liquid chromatography-tandem mass spectrometry to confirm the accuracy of the system. Results show good correlation between the two methods. This method is rapid, sensitive, specific, and convenient. It can be applied in the field for on-site detection of CLP in urine samples.
authors.affiliation: State Key Laboratory of Food Science and Technology, Nanchang University, 235 Nanjing East Road, Nanchang 330047, People's Republic of China.
authors.forename: Tao
authors.initials: T
authors.lastname: Peng
authors.affiliation: Jiangxi Agricultural Product Quality Safety and Inspection Center, 151 Wenjiao Road, Nanchang 330077, People's Republic of China.
authors.forename: Fu S
authors.initials: FS
authors.lastname: Zhang
authors.affiliation: Jiangxi Zodolabs Bioengineering Co., Ltd., 235 Nanjing East Road, Nanchang 330047, People's Republic of China.
authors.forename: Wan C
authors.initials: WC
authors.lastname: Yang
authors.affiliation: Jiangxi Agricultural Product Quality Safety and Inspection Center, 151 Wenjiao Road, Nanchang 330077, People's Republic of China.
authors.forename: Dong X
authors.initials: DX
authors.lastname: Li
authors.affiliation: Jiangxi Zodolabs Bioengineering Co., Ltd., 235 Nanjing East Road, Nanchang 330047, People's Republic of China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Food Science and Technology, Nanchang University, 235 Nanjing East Road, Nanchang 330047, People's Republic of China.
authors.forename: Yong H
authors.initials: YH
authors.lastname: Xiong
authors.affiliation: State Key Laboratory of Food Science and Technology, Nanchang University, 235 Nanjing East Road, Nanchang 330047, People's Republic of China.
authors.forename: Hua
authors.initials: H
authors.lastname: Wei
authors.affiliation: State Key Laboratory of Food Science and Technology, Nanchang University, 235 Nanjing East Road, Nanchang 330047, People's Republic of China. talktolaiwh@163.com.
authors.forename: Wei H
authors.initials: WH
authors.lastname: Lai
mesh_terms: D000818:Animals; D002138:Calibration; D002681:China; D002846:Chromatography, Affinity; D002853:Chromatography, Liquid; D003429:Cross Reactions; D004350:Drug Residues / Q000032:analysis*; D017979:Gold Colloid / Q000737:chemistry; D007545:Isoproterenol / Q000031:analogs & derivatives* / Q000652:urine; D008460:Meat; D015203:Reproducibility of Results; D013552:Swine; D053719:Tandem Mass Spectrometry; D016482:Urinalysis / Q000379:methods* / Q000662:veterinary*
chemical_list: D017979:Gold Colloid; C004350:clorprenaline; D007545:Isoproterenol