title: Effect of swimming on the production of aldosterone in rats.
abstract: It has been demonstrated that exercise is one of the stresses known to increase the aldosterone secretion. Both potassium and angiotensin II (Ang II) levels are shown to be correlated with aldosterone production during exercise, but the mechanism is still unclear. In an in vivo study, male rats were catheterized via right jugular vein (RJV), and divided into four groups namely water immersion, swimming, lactate infusion (13 mg/kg/min) and pyruvate infusion (13 mg/kg/min) groups. Each group was treated for 10 min. Blood samples were collected at 0, 10, 15, 30, 60 and 120 min from RJV after administration. In an in vitro study, rat zona glomerulosa (ZG) cells were challenged by lactate (1-10 mM) in the presence or absence of Ang II (10(-8) M) for 60 min. The levels of aldosterone in plasma and medium were measured by radioimmunoassay. Cell lysates were analyzed by immunoblotting assay. After exercise and lactate infusion, plasma levels of aldosterone and lactate were significantly higher than those in the control group. Swimming for 10 min significantly increased the plasma Ang II levels in male rats. Administration of lactate plus Ang II significantly increased aldosterone production and enhanced protein expression of steroidogenic acute regulatory protein (StAR) in ZG cells. These results demonstrated that acute exercise led to the increase of both aldosterone and Ang II secretion, which is associated with lactate action on ZG cells and might be dependent on the activity of renin-angiotensin system.
authors.affiliation: Department of Rehabilitation, Cheng Hsin General Hospital, Taipei, Taiwan, ROC.
authors.forename: Fu-Kong
authors.initials: FK
authors.lastname: Lieu
authors.affiliation: Department of Physiology, School of Medicine, National Yang-Ming University, Taipei, Taiwan, ROC.
authors.forename: Chih-Yung
authors.initials: CY
authors.lastname: Lin
authors.affiliation: Department of Physiology, School of Medicine, National Yang-Ming University, Taipei, Taiwan, ROC; Graduate Institute of Basic Medical Science, College of Medicine, China Medical University, and Medical Center of Aging Research, China Medical University Hospital, Taichung, Taiwan, ROC; Department of Biotechnology, College of Health Science, Asia University, Taichung, Taiwan, ROC; Department of Medical Research and Education, Taipei Veterans General Hospital, Taipei, Taiwan, ROC.
authors.forename: Paulus S
authors.initials: PS
authors.lastname: Wang
authors.affiliation: Department of Physiology, School of Medicine, National Yang-Ming University, Taipei, Taiwan, ROC.
authors.forename: Cai-Yun
authors.initials: CY
authors.lastname: Jian
authors.affiliation: Department of Physiology, School of Medicine, National Yang-Ming University, Taipei, Taiwan, ROC.
authors.forename: Yung-Hsing
authors.initials: YH
authors.lastname: Yeh
authors.affiliation: Department of Physiology, School of Medicine, National Yang-Ming University, Taipei, Taiwan, ROC.
authors.forename: Yi-An
authors.initials: YA
authors.lastname: Chen
authors.affiliation: Department of Physiology, School of Medicine, National Yang-Ming University, Taipei, Taiwan, ROC.
authors.forename: Kai-Lee
authors.initials: KL
authors.lastname: Wang
authors.affiliation: Department of Rehabilitation, Cheng Hsin General Hospital, Taipei, Taiwan, ROC.
authors.forename: Yi-Chun
authors.initials: YC
authors.lastname: Lin
authors.affiliation: Department of Chemical Engineering, College of Engineering, Chinese Culture University, Taipei, Taiwan, ROC.
authors.forename: Ling-Ling
authors.initials: LL
authors.lastname: Chang
authors.affiliation: Graduate Institute of Clinical Medical Science, China Medical University, Taichung, Taiwan, ROC; Department of Medical Research, China Medical University Hospital, Taichung, Taiwan, ROC; Department of Health and Nutrition Biotechnology, Asia University, Taichung, Taiwan, ROC.
authors.forename: Guei-Jane
authors.initials: GJ
authors.lastname: Wang
authors.affiliation: Department of Physiology and Pharmacology, College of Medicine, Chang-Gung University, Taoyuan, Taiwan, ROC.
authors.forename: Shyi-Wu
authors.initials: SW
authors.lastname: Wang
mesh_terms: D000450:Aldosterone / Q000097:blood*; D000804:Angiotensin II / Q000097:blood* / Q000494:pharmacology; D000818:Animals; D002405:Catheterization, Central Venous; D007101:Immersion; D019344:Lactic Acid / Q000097:blood* / Q000494:pharmacology; D008297:Male; D010750:Phosphoproteins / Q000096:biosynthesis; D061251:Primary Cell Culture; D019289:Pyruvic Acid / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D012084:Renin-Angiotensin System / Q000187:drug effects / Q000502:physiology; D013550:Swimming; D014867:Water; D015384:Zona Glomerulosa / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D000098682:Steroidogenic Acute Regulatory Protein
chemical_list: D010750:Phosphoproteins; D000098682:Steroidogenic Acute Regulatory Protein; D014867:Water; D000804:Angiotensin II; D019344:Lactic Acid; D000450:Aldosterone; D019289:Pyruvic Acid