title: Protein tyrosine phosphatase PTPN22 +1858C/T polymorphism is associated with active vitiligo.
abstract: Vitiligo is characterized by a skin depigmentation disorder resulting from an autoimmune response targeting melanocytes. Within the genetic factors involved in the development of the vitiligo immune response, various genes in the major histocompatibility complex (MHC) and non-MHC loci have been considered to be risk factors. ThePTPN22gene encodes for a lymphoid protein tyrosine phosphatase, a regulator of the activation and development of T-cells. The+1858C/Tpolymorphism has been associated to autoimmune disease susceptibility in different populations and could be implicated in the onset of vitiligo. To assess the possible association between the presence ofPTPN22 +1858C/Tand vitiligo, 187 patients with vitiligo and 223 control subjects were analyzed in the study. Genomic DNA was isolated using the salting-out method and samples were subjected to polymerase chain reaction-restriction fragment length polymorphism in order to detect thePTPN22 +1858C/Tpolymorphism. Causal associations were determined by χ2test and their respective odds ratio (OR) was assessed in a 2×2 contingency table. The results showed an association between active vitiligo and the allele T load [P=0.0418; OR, 2.5706; 95% confidence interval (CI), 1.0040-6.5816], and active vitiligo-CT genotype (P=0.0389, OR, 2.6548; 95% CI, 1.0191-6.9156). In conclusion, the present data indicates a possible association between thePTPN22 +1858C/Tgenotype and a significant susceptibility of developing an active form of vitiligo.
authors.affiliation: Dermatology Service, Hospital Universitario 'Dr. José Eleuterio González', Monterrey, CP 64460, Nuevo León, Mexico.
authors.forename: Martha Elena
authors.initials: ME
authors.lastname: Garcia-Melendez
authors.affiliation: Department of Biochemistry and Molecular Medicine, Faculty of Medicine, Universidad Autónoma de Nuevo León, Monterrey, CP 64460, Nuevo León, Mexico ; Saltillo Unit Faculty of Medicine, Universidad Autónoma de Coahuila, Saltillo CP 25000, Coahuila, Mexico.
authors.forename: Mauricio
authors.initials: M
authors.lastname: Salinas-Santander
authors.affiliation: Department of Biochemistry and Molecular Medicine, Faculty of Medicine, Universidad Autónoma de Nuevo León, Monterrey, CP 64460, Nuevo León, Mexico.
authors.forename: Celia
authors.initials: C
authors.lastname: Sanchez-Dominguez
authors.affiliation: Department of Biochemistry and Molecular Medicine, Faculty of Medicine, Universidad Autónoma de Nuevo León, Monterrey, CP 64460, Nuevo León, Mexico.
authors.forename: Hugo
authors.initials: H
authors.lastname: Gonzalez-Cardenas
authors.affiliation: Nursery School Faculty, Universidad Autónoma de Nuevo León, Monterrey, CP 64460, Nuevo León, Mexico.
authors.forename: Ricardo M
authors.initials: RM
authors.lastname: Cerda-Flores
authors.affiliation: Dermatology Service, Hospital Universitario 'Dr. José Eleuterio González', Monterrey, CP 64460, Nuevo León, Mexico.
authors.forename: Jorge
authors.initials: J
authors.lastname: Ocampo-Candiani
authors.affiliation: Department of Biochemistry and Molecular Medicine, Faculty of Medicine, Universidad Autónoma de Nuevo León, Monterrey, CP 64460, Nuevo León, Mexico ; Molecular Biology, Genomics and Sequencing Unit, Center for Research and Development in the Health Sciences, Universidad Autónoma de Nuevo León, Monterrey, CP 64460, Nuevo León, Mexico.
authors.forename: Rocío
authors.initials: R
authors.lastname: Ortiz-López
keywords: Mexican population; PTPN22 +1858C/T; autoimmune diseases; lymphoid protein tyrosine phosphatase; polymerase chain reaction-restriction fragment length polymorphism; polymorphism; vitiligo