title: Epigallocatechin gallate potentially attenuates Fluoride induced oxidative stress mediated cardiotoxicity and dyslipidemia in rats.
abstract: The present study was undertaken to evaluate the cardioprotective role of (-)-epigallocatechin-gallate (EGCG) against Fluoride (F) induced oxidative stress mediated cardiotoxicity in rats. The animals exposed to F as sodium Fluoride (NaF) (25mg/kg BW) for 4 weeks exhibited a significant increase in the levels of cardiac troponins T and I (cTnT & I), cardiac serum markers, lipid peroxidative markers and plasma total cholesterol (TC), triglycerides (TG), phospholipids (PL), free fatty acids (FFA), low density lipoprotein cholesterol, very low density lipoprotein cholesterol as well as cardiac lipids profile (TC, TG and FFA) with the significant decrease of high density lipoprotein cholesterol and cardiac phospholipids. F intoxication also decreased the levels of mitochondrial enzymes such as ICDH, SDH, MDH, α-KGDH and NADH in the cardiac tissue of rats. The mitochondrial Ca(2+) ion level was also significantly reduced along with the significant decrease in the levels of enzymatic and non enzymatic antioxidants. Furthermore, F treatment significantly increased the DNA fragmentation, up regulate cardiac pro-apoptotic markers, inflammatory markers and down-regulate the anti-apoptotic markers in the cardiac tissue. Pre administration of EGCG (40mg/kg/bw) in F intoxicated rats remarkably recovered all these altered parameters to near normalcy through its antioxidant nature. Thus, results of the present study clearly demonstrated that treatment with EGCG prior to F intoxication has a significant role in protecting F-induced cardiotoxicity and dyslipidemia in rats.
authors.affiliation: Department of Zoology, Annamalai University, Annamalainagar 608002, Tamilnadu, India. Electronic address: smprabu73@gmail.com.
authors.forename: S
authors.initials: S
authors.lastname: Miltonprabu
authors.affiliation: Department of Zoology, Annamalai University, Annamalainagar 608002, Tamilnadu, India.
authors.forename: S
authors.initials: S
authors.lastname: Thangapandiyan
mesh_terms: D000251:Adenosine Triphosphatases / Q000378:metabolism; D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D015415:Biomarkers / Q000097:blood; D001835:Body Weight / Q000187:drug effects; D002118:Calcium / Q000378:metabolism; D066126:Cardiotoxicity / Q000097:blood / Q000188:drug therapy* / Q000473:pathology; D002392:Catechin / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use; D053938:DNA Fragmentation / Q000187:drug effects; D004327:Drinking Behavior / Q000187:drug effects; D050171:Dyslipidemias / Q000097:blood / Q000188:drug therapy* / Q000473:pathology; D005247:Feeding Behavior / Q000187:drug effects; D005459:Fluorides / Q000633:toxicity*; D015227:Lipid Peroxidation / Q000187:drug effects; D008055:Lipids / Q000097:blood; D008297:Male; D008566:Membranes / Q000187:drug effects / Q000201:enzymology; D008929:Mitochondria, Heart / Q000187:drug effects / Q000201:enzymology; D009206:Myocardium / Q000473:pathology / Q000648:ultrastructure; D009929:Organ Size / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000975:Antioxidants; D015415:Biomarkers; D008055:Lipids; D002392:Catechin; C045651:epigallocatechin gallate; D000251:Adenosine Triphosphatases; D005459:Fluorides; D002118:Calcium
keywords: EGCG; Fluoride; Heart; Lipids; Oxidative stress