title: Cystathionine-gamma-lyase inhibitor attenuates acute lung injury induced by acute pancreatitis in rats.
abstract: BACKGROUND
  Acute pancreatitis (AP) is known to induce injuries to extrapancreatic organs. Because respiratory dysfunction is the main cause of death in patients with severe AP, acute pancreatitis-associated lung injury (APALI) is a great challenge for clinicians. This study aimed to investigate the potential role of hydrogen sulfide (H2S) in the pathogenesis of APALI.
  
  
  METHODS
  Fifty-four SD rats were randomly divided into three groups: the AP group of rats that received injection of sodium deoxycholate into the common bile duct, the control group that underwent a sham operation, and the treatment group made by intraperitoneal injection of propargylglycine (PAG), an inhibitor of cystathionine-γ-lyase (CSE), into rats with AP. Histopathology of the lung was examined and the expression of CSE and TNF-α mRNA in lung tissue was detected by real-time polymerase chain reaction. The H2S level in the serum was detected spectrophotometrically.
  
  
  RESULTS
  The serum concentration of H2S and CSE and TNF-α expression in the lung were increased in AP rats modeled after 3 h and 6 h than in control rats (p < 0.05). Intraperitoneal injection of PAG could reduce the serum concentration of H2S, reduce CSE and TNF-α expression, and alleviate the lung pathology (p < 0.05).
  
  
  CONCLUSIONS
  Taken together, our findings suggest that the H2S/CSE system is crucially involved in the pathological process of APALI and represents a novel target for the therapy of APALI.
authors.affiliation: Department of General Surgery, Tumor Hospital, Fourth Affiliated Hospital of Soochow University, Changzhou, Jiangsu Province, P.R. China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Qu
authors.affiliation: Department of Hepatobiliary Surgery, Changzhou 1 People's Hospital, Third Affiliated Hospital of Soochow University, Changzhou, Jiangsu Province, P.R. China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Jiang
authors.affiliation: Department of Hepatobiliary Surgery, Changzhou 1 People's Hospital, Third Affiliated Hospital of Soochow University, Changzhou, Jiangsu Province, P.R. China.
authors.forename: Bao-Qiang
authors.initials: BQ
authors.lastname: Wu
authors.affiliation: Department of Hepatobiliary Surgery, Changzhou 1 People's Hospital, Third Affiliated Hospital of Soochow University, Changzhou, Jiangsu Province, P.R. China.
authors.forename: Yun-Fei
authors.initials: YF
authors.lastname: Duan
authors.affiliation: Department of Hepatobiliary Surgery, Changzhou 1 People's Hospital, Third Affiliated Hospital of Soochow University, Changzhou, Jiangsu Province, P.R. China.
authors.forename: Zhen-Di
authors.initials: ZD
authors.lastname: Sun
authors.affiliation: Department of Hepatobiliary Surgery, Changzhou 1 People's Hospital, Third Affiliated Hospital of Soochow University, Changzhou, Jiangsu Province, P.R. China.
authors.forename: Guang-Hua
authors.initials: GH
authors.lastname: Luo
keywords: acute pancreatitis associated lung injury; hydrogen sulfide; propargylglycine