title: Backbone assignments of mini-RecA intein with short native exteins and an active N-terminal catalytic cysteine.
abstract: The backbone resonance assignments of an engineered splicing-inactive mini-RecA intein based on triple resonance experiments with [(13)C,(15)N]-labeled protein are reported. The construct contains inactivating mutations specifically designed to retain most catalytic residues, especially those that are potentially metal-coordinating. The assignments are essential for protein structure determination of a precursor with an active N-terminal catalytic cysteine and for investigation of the atomic details of splicing.
authors.affiliation: Howard P. Isermann Department of Chemical and Biological Engineering and The Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, NY, 12180, USA.
authors.forename: C Seth
authors.initials: CS
authors.lastname: Pearson
authors.affiliation: Howard P. Isermann Department of Chemical and Biological Engineering and The Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, NY, 12180, USA.
authors.forename: Georges
authors.initials: G
authors.lastname: Belfort
authors.affiliation: Department of Biological Sciences and The RNA Institute, University at Albany, Albany, NY, 12222, USA. mbelfort@albany.edu.
authors.forename: Marlene
authors.initials: M
authors.lastname: Belfort
authors.affiliation: Department of Chemistry, University at Albany, Albany, NY, 12222, USA.
authors.forename: Alexander
authors.initials: A
authors.lastname: Shekhtman
mesh_terms: D055162:Biocatalysis; D003545:Cysteine / Q000737:chemistry*; D047669:Exteins; D047668:Inteins; D019906:Nuclear Magnetic Resonance, Biomolecular; D015202:Protein Engineering; D066244:Proton Magnetic Resonance Spectroscopy; D011938:Rec A Recombinases / Q000737:chemistry*
chemical_list: D011938:Rec A Recombinases; D003545:Cysteine
keywords: Intein; Protein splicing; Tuberculosis