title: Periostin is temporally expressed as an extracellular matrix component in skeletal muscle regeneration and differentiation.
abstract: The transcriptional events and pathways responsible for the acquisition of the myogenic phenotype during regeneration and myogenesis have been studied extensively. The modulators that shape the extracellular matrix in health and disease, however, are less understood. Understanding the components and pathways of this remodeling will aid the restoration of the architecture and prevent deterioration under pathological conditions such as fibrosis. Periostin, a matricellular protein associated with remodeling of the extracellular matrix and connective tissue architecture, is emerging in pathological conditions associated with fibrosis in adult life. Periostin also complicates fibrosis in degenerative skeletal muscle conditions such as dystrophies. This study primarily addresses the spatial and temporal involvement of periostin along skeletal muscle regeneration. In the acute skeletal muscle injury model that shows recovery without fibrosis, we show that periostin is rapidly disrupted along with the extensive necrosis and periostin mRNA is transiently upregulated during the myotube maturation. This expression is stringently initiated from the newly regenerating fibers. However, this observation is contrasting to a model that displays extensive fibrosis where upregulation of periostin expression is stable and confined to the fibrotic compartments of endomysial and perimysial space. In vitro myoblast differentiation further supports the claim that upregulation of periostin expression is a function of extracellular matrix remodeling during myofiber differentiation and maturation. We further seek to identify the expression kinetics of various periostin isoforms during the differentiation of rat and mouse myoblasts. Results depict that a singular periostin isoform dominated the rat muscle, contrasting to multiple isoforms in C2C12 myoblast cells. This study shows that periostin, a mediator with deleterious impact on conditions exhibiting fibrosis, is also produced and secreted by myoblasts and regenerating myofibers during architectural remodeling in the course of development and regeneration.
authors.affiliation: Dept. of Medical Biology, Hacettepe University School of Medicine, Ankara, Turkey.
authors.forename: Cansu
authors.initials: C
authors.lastname: Ozdemir
authors.affiliation: Dept. of Medical Biology, Hacettepe University School of Medicine, Ankara, Turkey.
authors.forename: Uğur
authors.initials: U
authors.lastname: Akpulat
authors.affiliation: Dept. of Medical Biology, Hacettepe University School of Medicine, Ankara, Turkey.
authors.forename: Parisa
authors.initials: P
authors.lastname: Sharafi
authors.affiliation: Dept. of Medical Biology, Hacettepe University School of Medicine, Ankara, Turkey.
authors.forename: Yılmaz
authors.initials: Y
authors.lastname: Yıldız
authors.affiliation: Laboratory Animal Breeding and Research Unit, Hacettepe University School of Medicine, Ankara, Turkey.
authors.forename: Ilyas
authors.initials: I
authors.lastname: Onbaşılar
authors.affiliation: Dept. of Medical Biology, Hacettepe University School of Medicine, Ankara, Turkey. Electronic address: kocaefe@hacettepe.edu.tr.
authors.forename: Cetin
authors.initials: C
authors.lastname: Kocaefe
mesh_terms: D000818:Animals; D001483:Base Sequence; D015153:Blotting, Western; D015815:Cell Adhesion Molecules / Q000235:genetics* / Q000378:metabolism; D002454:Cell Differentiation; D017931:DNA Primers; D016326:Extracellular Matrix Proteins / Q000235:genetics* / Q000378:metabolism; D008297:Male; D018482:Muscle, Skeletal / Q000166:cytology / Q000378:metabolism* / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D012038:Regeneration; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D015815:Cell Adhesion Molecules; D017931:DNA Primers; D016326:Extracellular Matrix Proteins; C512836:Postn protein, rat
keywords: Muscle injury; Muscle regeneration; Myogenic differentiation; Periostin