title: The effect of deferoxamine on angiogenesis and bone repair in steroid-induced osteonecrosis of rabbit femoral heads.
abstract: In this study, we examined whether local deferoxamine (DFO) administration can promote angiogenesis and bone repair in steroid-induced osteonecrosis of the femoral head (ONFH). Steroid-induced ONFH was induced in 65 mature male New Zealand white rabbits by methylprednisolone in combination with lipopolysaccharide. Six weeks later, the rabbits received no treatment (model group, N = 15), bilateral core decompression (CD group, N = 20) or CD in combination with local DFO administration (DFO group, N = 20). Six weeks after the surgery, vascularization in the femoral head was evaluated by ink artery infusion angiography and immunohistochemical staining for von Willebrand Factor (vWF). Bone repair was assessed by histologic analysis and micro-computed tomography (micro-CT). Immunohistochemical staining was performed to analyze the expression of vascular endothelial growth factor (VEGF), hypoxia-inducible factor-1α (HIF-1α), bone morphogenetic protein-2 (BMP-2), and osteocalcin (OCN). Ink artery infusion angiography and microvessel analysis by immuohistochemical staining for vWF showed more blood vessels in the DFO group than other groups. The expression of HIF-1α, VEGF, BMP-2, and OCN, indicated by immunohistochemical staining, was higher in the DFO group compared with other groups. Micro-CT scanning results indicated that the DFO group had larger volume of newly formed bone than the CD group. This work indicated that local DFO administration improved angiogenesis and bone repair of early stage ONFH in rabbit model, and it may offer an efficient, economic, and simple therapy for early stage ONFH.
authors.affiliation: The First Department of Orthopedics, The Second Affiliated Hospital of Medical School of Xi'an Jiaotong University, Xiwu Road, Xi'an, Shaanxi Province 710004, China.
authors.forename: Jia
authors.initials: J
authors.lastname: Li
authors.affiliation: The First Department of Orthopedics, The Second Affiliated Hospital of Medical School of Xi'an Jiaotong University, Xiwu Road, Xi'an, Shaanxi Province 710004, China.
authors.forename: Lihong
authors.initials: L
authors.lastname: Fan
authors.affiliation: The First Department of Orthopedics, The Second Affiliated Hospital of Medical School of Xi'an Jiaotong University, Xiwu Road, Xi'an, Shaanxi Province 710004, China.
authors.forename: Zefeng
authors.initials: Z
authors.lastname: Yu
authors.affiliation: The First Department of Orthopedics, The Second Affiliated Hospital of Medical School of Xi'an Jiaotong University, Xiwu Road, Xi'an, Shaanxi Province 710004, China.
authors.forename: Xiaoqian
authors.initials: X
authors.lastname: Dang
authors.affiliation: The First Department of Orthopedics, The Second Affiliated Hospital of Medical School of Xi'an Jiaotong University, Xiwu Road, Xi'an, Shaanxi Province 710004, China wkzh1955@163.com.
authors.forename: Kunzheng
authors.initials: K
authors.lastname: Wang
mesh_terms: D000818:Animals; D055396:Bone Morphogenetic Protein 2 / Q000378:metabolism; D001861:Bone Regeneration / Q000187:drug effects*; D003676:Deferoxamine / Q000494:pharmacology*; D004195:Disease Models, Animal; D005270:Femur Head / Q000000981:diagnostic imaging / Q000378:metabolism* / Q000473:pathology; D005938:Glucocorticoids / Q000009:adverse effects* / Q000494:pharmacology; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000378:metabolism; D008297:Male; D008775:Methylprednisolone / Q000009:adverse effects* / Q000494:pharmacology; D018919:Neovascularization, Physiologic / Q000187:drug effects*; D015675:Osteocalcin / Q000378:metabolism; D010020:Osteonecrosis / Q000139:chemically induced / Q000000981:diagnostic imaging / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D011817:Rabbits; D017262:Siderophores / Q000494:pharmacology*; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism; D055114:X-Ray Microtomography; D014841:von Willebrand Factor / Q000378:metabolism
chemical_list: D055396:Bone Morphogenetic Protein 2; D005938:Glucocorticoids; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D017262:Siderophores; D042461:Vascular Endothelial Growth Factor A; D014841:von Willebrand Factor; D015675:Osteocalcin; D003676:Deferoxamine; D008775:Methylprednisolone
keywords: Osteonecrosis; angiogenesis; bone repair; deferoxamine; hypoxia-inducible factor-1α