title: Apolipoprotein E isoforms disrupt long-chain fatty acid distribution in the plasma, the liver and the adipose tissue of mice.
abstract: Evidences suggest that omega-3 fatty acid (n-3 PUFA) metabolism is imbalanced in apolipoprotein E epsilon 4 isoform carriers (APOE4). This study aimed to investigate APOE genotype-dependant modulation of FA profiles, protein and enzyme important to fatty acid (FA) metabolism in the adipose tissue, the liver and the plasma using human APOE-targeted replacement mouse-model (N=37). FA transport (FATP) and binding (FABP) protein levels in tissues and concentrations of liver carnitine palmitoyltransferase 1 (CPT1) were performed. N-3 PUFA concentration was >45% lower in the adipose tissue and liver of APOE4 mice compared to APOE3 mice. In APOE4 mice, there were higher levels of FATP and FABP in the liver and higher FATP in the adipose tissue compared to APOE2 mice. There was a trend towards higher CPT1 concentrations in APOE4 mice compared to APOE3 mice. Therefore, since APOE-isoform differences were not always in line with the unbalanced n-3 PUFA profiles in organs, other proteins may be involved in maintaining n-3 PUFA homeostasis in mice with different APOE-isoforms.
authors.affiliation: Research Center on Aging, Health and Social Services Center, University Institute of Geriatrics of Sherbrooke, Sherbrooke, Canada J1H 4C4; Departement of Medicine, Université de Sherbrooke, Sherbrooke, Canada; Institute of Nutrition and Functional Foods, Université Laval, Québec, Canada.
authors.forename: Valérie
authors.initials: V
authors.lastname: Conway
authors.affiliation: Research Center on Aging, Health and Social Services Center, University Institute of Geriatrics of Sherbrooke, Sherbrooke, Canada J1H 4C4.
authors.forename: Annie
authors.initials: A
authors.lastname: Larouche
authors.affiliation: Faculty of pharmacy, Université Laval and CHU-Q Research Centre, Québec, Canada.
authors.forename: Wael
authors.initials: W
authors.lastname: Alata
authors.affiliation: Institute of Nutrition and Functional Foods, Université Laval, Québec, Canada; Faculty of pharmacy, Université Laval and CHU-Q Research Centre, Québec, Canada.
authors.forename: Milène
authors.initials: M
authors.lastname: Vandal
authors.affiliation: Institute of Nutrition and Functional Foods, Université Laval, Québec, Canada; Faculty of pharmacy, Université Laval and CHU-Q Research Centre, Québec, Canada.
authors.forename: Frédéric
authors.initials: F
authors.lastname: Calon
authors.affiliation: Research Center on Aging, Health and Social Services Center, University Institute of Geriatrics of Sherbrooke, Sherbrooke, Canada J1H 4C4; Departement of Medicine, Université de Sherbrooke, Sherbrooke, Canada; Institute of Nutrition and Functional Foods, Université Laval, Québec, Canada. Electronic address: melanie.plourde2@usherbrooke.ca.
authors.forename: Mélanie
authors.initials: M
authors.lastname: Plourde
mesh_terms: D000273:Adipose Tissue / Q000737:chemistry*; D000818:Animals; D053329:Apolipoprotein E2 / Q000235:genetics; D053318:Apolipoprotein E3 / Q000235:genetics; D053327:Apolipoprotein E4 / Q000235:genetics; D001057:Apolipoproteins E / Q000235:genetics*; D002334:Carnitine O-Palmitoyltransferase / Q000032:analysis; D050612:Fatty Acid Transport Proteins / Q000032:analysis; D050556:Fatty Acid-Binding Proteins / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis* / Q000097:blood*; D015525:Fatty Acids, Omega-3 / Q000097:blood; D005260:Female; D005838:Genotype; D006801:Humans; D008099:Liver / Q000737:chemistry*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D020033:Protein Isoforms
chemical_list: D053329:Apolipoprotein E2; D053318:Apolipoprotein E3; D053327:Apolipoprotein E4; D001057:Apolipoproteins E; C493320:Fabp1 protein, mouse; C493411:Fabp4 protein, mouse; D050612:Fatty Acid Transport Proteins; D050556:Fatty Acid-Binding Proteins; D005227:Fatty Acids; D015525:Fatty Acids, Omega-3; D020033:Protein Isoforms; C493569:Slc27a1 protein, mouse; C113098:Slc27a5 protein, mouse; D002334:Carnitine O-Palmitoyltransferase
keywords: APOE4 carriers.; Cellular FA uptake and degradation; Transport and binding proteins; n-3 PUFA metabolism