title: Probing protein targeting to plasmodesmata using fluorescence recovery after photo-bleaching.
abstract: Fluorescence recovery after photo-bleaching (FRAP) involves the irreversible bleaching of a fluorescent protein within a specific area of the cell using a high-intensity laser. The recovery of fluorescence represents the movement of new protein into this area and can therefore be used to investigate factors involved in this movement. Here we describe a FRAP method to investigate the effect of a range of pharmacological agents on the targeting of Tobacco mosaic virus movement protein to plasmodesmata.
authors.affiliation: Cell and Molecular Sciences Group, The James Hutton Institute, Invergowrie, Dundee, DD2 5DA, UK, Kath.Wright@hutton.ac.uk.
authors.forename: Kathryn M
authors.initials: KM
authors.lastname: Wright
authors.forename: Katrin M
authors.initials: KM
authors.lastname: MacKenzie
mesh_terms: D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D020126:Brefeldin A / Q000494:pharmacology; D019086:Bridged Bicyclo Compounds, Heterocyclic / Q000494:pharmacology; D003571:Cytochalasin B / Q000494:pharmacology; D004136:Dinitrobenzenes / Q000494:pharmacology; D036681:Fluorescence Recovery After Photobleaching / Q000379:methods*; D018506:Gene Expression Regulation, Plant; D005822:Genetic Vectors; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D008562:Membrane Glycoproteins / Q000235:genetics / Q000378:metabolism; D008840:Microfilament Proteins / Q000235:genetics / Q000378:metabolism; D018613:Microscopy, Confocal; D030821:Plants, Genetically Modified; D031425:Plasmodesmata / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology; D021381:Protein Transport / Q000187:drug effects; D011960:Receptors, Estrogen / Q000235:genetics / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D036226:Seedlings / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D013424:Sulfanilamides / Q000494:pharmacology; D053778:Thiazolidines / Q000494:pharmacology; D014026:Nicotiana; D014027:Tobacco Mosaic Virus / Q000235:genetics* / Q000378:metabolism; D014404:Tubulin / Q000235:genetics / Q000378:metabolism; D050257:Tubulin Modulators / Q000494:pharmacology
chemical_list: D019086:Bridged Bicyclo Compounds, Heterocyclic; D004136:Dinitrobenzenes; D008562:Membrane Glycoproteins; D008840:Microfilament Proteins; D011500:Protein Synthesis Inhibitors; D011960:Receptors, Estrogen; D011993:Recombinant Fusion Proteins; D013424:Sulfanilamides; D053778:Thiazolidines; D014404:Tubulin; D050257:Tubulin Modulators; C026742:plastin; D049452:Green Fluorescent Proteins; D020126:Brefeldin A; D003571:Cytochalasin B; C012465:oryzalin; C037068:latrunculin B