title: Regulation of ku70-bax complex in cells.
abstract: Ku70, a DNA repair factor in the nucleus, also regulates cell death by binding to the apoptotic protein Bax in the cytoplasm. Acetylation of Ku70 triggers Bax release resulting in Bax dependent cell death. Thus dissociating Bax from Ku70, either by inhibiting histone deacetylase 6 (HDAC6) that deacetylates Ku70 or by increasing Ku70 acetylation induces cell death. Our results showed that in neuroblastoma cells, the depletion of Ku70 results in Bax-dependent cell death. This model provides a rationale for screening Ku70 acetylation modulators that can be tested in clinical trials, either alone or in combination with radiotherapy or DNA-damaging agents for the treatment of cancer.
authors.affiliation: Departments of Obstetrics and Gynecology, and Biological Chemistry, University of Michigan Medical School, Ann Arbor, MI, USA.
authors.forename: Manila
authors.initials: M
authors.lastname: Hada
authors.affiliation: Departments of Obstetrics and Gynecology, and Biological Chemistry, University of Michigan Medical School, Ann Arbor, MI, USA.
authors.forename: Roland Ps
authors.initials: RP
authors.lastname: Kwok
keywords: Bax; CBP; HDAC6; Ku70; apoptosis