title: Five miRNAs as novel diagnostic biomarker candidates for primary nasopharyngeal carcinoma.
abstract: MicroRNAs (miRNAs) play an essential role in the development and progression of nasopharyngeal carcinomas (NPC). Despite advances in the field of cancer molecular biology and biomarker discovery, the development of clinically validated biomarkers for primary NPC has remained elusive. In this study, we investigated the expression and clinical significance of miRNAs as novel primary NPC diagnostic biomarkers. We used an array containing 2, 500 miRNAs to identify 22 significant miRNAs, and these candidate miRNAs were validated using 67 fresh NPC and 25 normal control tissues via quantitative real-time PCR (qRT-PCR). Expression and correlation analyses were performed with various statistical approaches, in addition to logistic regression and receiver operating characteristic curve analyses to evaluate diagnostic efficacy. qRT-PCR revealed five differentially expressed miRNAs (miR-93-5p, miR-135b-5p, miR-205-5p and miR-183-5p) in NPC tissue samples relative to control samples (p<0.05), with miR-135b-5p and miR-205-5p being of significant diagnostic value (p<0.01). Moreover, comparison of NPC patient clinicopathologic data revealed a negative correlation between miR-93-5p and miR- 183-5p expression levels and lymph node status (p<0.05). These findings display an altered expression of many miRNAs in NPC tissues, thus providing information pertinent to pathophysiological and diagnostic research. Ultimately, miR-135b-5p and miR-205-5p may be implicated as novel NPC candidate biomarkers, while miR- 93-5p, miR-650 and miR-183-5p may find application as relevant clinical pathology and diagnostic candidate biomarkers.
authors.affiliation: Clinical Research Center, Guangdong Medical College, Zhanjiang, ChinaE-mail : gangliu11@gdmc.edu.cn.
authors.forename: Jin-Feng
authors.initials: JF
authors.lastname: Tang
authors.forename: Zhong-Hua
authors.initials: ZH
authors.lastname: Yu
authors.forename: Tie
authors.initials: T
authors.lastname: Liu
authors.forename: Zi-Ying
authors.initials: ZY
authors.lastname: Lin
authors.forename: Ya-Hong
authors.initials: YH
authors.lastname: Wang
authors.forename: La-Wei
authors.initials: LW
authors.lastname: Yang
authors.forename: Hui-Juan
authors.initials: HJ
authors.lastname: He
authors.forename: Jun
authors.initials: J
authors.lastname: Cao
authors.forename: Hai-Li
authors.initials: HL
authors.lastname: Huang
authors.forename: Gang
authors.initials: G
authors.lastname: Liu
mesh_terms: D000328:Adult; D014408:Biomarkers, Tumor / Q000235:genetics*; D002277:Carcinoma; D016022:Case-Control Studies; D005260:Female; D005500:Follow-Up Studies; D020869:Gene Expression Profiling; D006801:Humans; D007124:Immunoenzyme Techniques; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D000077274:Nasopharyngeal Carcinoma; D009303:Nasopharyngeal Neoplasms / Q000175:diagnosis* / Q000235:genetics*; D009305:Nasopharynx / Q000378:metabolism; D009367:Neoplasm Staging; D011379:Prognosis; D012372:ROC Curve; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D014408:Biomarkers, Tumor; D035683:MicroRNAs