title: Mouse neutrophils express functional umami taste receptor T1R1/T1R3.
abstract: Neutrophils play an important role in the initiation of innate immunity against infection and injury. Although many different types of G-protein coupled receptors are functionally expressed in neutrophils, no reports have demonstrated functional expression of umami taste receptor in these cells. We observed that mouse neutrophils express the umami taste receptor T1R1/T1R3 through RNA sequencing and quantitative RT-PCR analysis. Stimulation of mouse neutrophils with L-alanine or L-serine, which are ligands for the umami taste receptor, elicited not only ERK or p38 MAPK phosphorylation but also chemotactic migration. Moreover, addition of L-alanine or L-serine markedly reduced the production of several cytokines including TNF-α induced by lipopolysaccharide (LPS) through inhibition of NF-κB activity or STAT3 phosphorylation in neutrophils. Our findings demonstrate that neutrophils express the umami taste receptor, through which tastants stimulate neutrophils, resulting in chemotactic migration, and attenuation of LPS-induced inflammatory response.
authors.affiliation: Department of Brain Science, Daegu Gyeongbuk Institute of Science and Technology (DGIST), Daegu 711-873, Korea.
authors.forename: NaHye
authors.initials: N
authors.lastname: Lee
authors.affiliation: Department of Biological Sciences, Sungkyunkwan University, Suwon 440-746, Korea.
authors.forename: Young Su
authors.initials: YS
authors.lastname: Jung
authors.affiliation: Department of Biological Sciences, Sungkyunkwan University, Suwon 440-746; Mitochondria Hub Regulation Center, Dong-A University, Busan 602-714, Korea.
authors.forename: Ha Young
authors.initials: HY
authors.lastname: Lee
authors.affiliation: Department of Brain Science, Daegu Gyeongbuk Institute of Science and Technology (DGIST), Daegu 711-873, Korea.
authors.forename: NaNa
authors.initials: N
authors.lastname: Kang
authors.affiliation: Department of Biological Sciences, Sungkyunkwan University, Suwon 440-746, Korea.
authors.forename: Yoo Jung
authors.initials: YJ
authors.lastname: Park
authors.affiliation: Department of Agricultural Biology, National Academy of Agricultural Science, RDA, Wanju 565-851, Korea.
authors.forename: Jae Sam
authors.initials: JS
authors.lastname: Hwang
authors.affiliation: Department of Biotechnology, Konkuk University, Chungju 380-701, Korea.
authors.forename: Young Yil
authors.initials: YY
authors.lastname: Bahk
authors.affiliation: Department of Brain Science, Daegu Gyeongbuk Institute of Science and Technology (DGIST), Daegu 711-873, Korea.
authors.forename: JaeHyung
authors.initials: J
authors.lastname: Koo
authors.affiliation: Department of Biological Sciences, Sungkyunkwan University, Suwon 440-746; Mitochondria Hub Regulation Center, Dong-A University, Busan 602-714; Samsung Advanced Institute for Health Sciences and Technology, Sungkyunkwan University, Seoul 135-710, Korea.
authors.forename: Yoe-Sik
authors.initials: YS
authors.lastname: Bae
mesh_terms: D000409:Alanine / Q000494:pharmacology; D000818:Animals; D002118:Calcium / Q000378:metabolism; D002465:Cell Movement / Q000187:drug effects; D002633:Chemotaxis / Q000187:drug effects; D016207:Cytokines / Q000378:metabolism; D005786:Gene Expression Regulation; D008070:Lipopolysaccharides / Q000633:toxicity; D051379:Mice; D016328:NF-kappa B / Q000037:antagonists & inhibitors / Q000378:metabolism; D009504:Neutrophils / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D010766:Phosphorylation; D012313:RNA / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D043562:Receptors, G-Protein-Coupled / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D050796:STAT3 Transcription Factor / Q000037:antagonists & inhibitors / Q000378:metabolism; D017423:Sequence Analysis, RNA; D012694:Serine / Q000494:pharmacology; D015398:Signal Transduction / Q000187:drug effects; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D016207:Cytokines; D008070:Lipopolysaccharides; D016328:NF-kappa B; D043562:Receptors, G-Protein-Coupled; D050796:STAT3 Transcription Factor; D014409:Tumor Necrosis Factor-alpha; C431926:taste receptors, type 1; D012694:Serine; D012313:RNA; D000409:Alanine; D002118:Calcium