title: Wild and attenuated vaccine RS-12 strains of mumps virus exhibit differences in amino acid sequences of their proteins.
abstract: Attenuated mumps virus (MuV) RS-12 strain-based vaccine is one of several effective vaccines available in the prevention of mumps. Since previous studies have unveiled only about one-third of the attenuated vaccine RS-12 strain genome sequence, the rest of sequence and molecular basis for attenuation remained unsolved. Therefore, in this study, the full-length genome sequences of wild and attenuated RS-12 strains were determined and compared. The comparison revealed nucleotide substitutions at 9 positions leading to amino acid substitutions at 6 positions in P, V, I, M, and L proteins, while the remaining substitutions were silent. This result indicates that the observed mutations in P, V, I, M, and L proteins of MuV might be responsible for the attenuation of the RS-12 vaccine strain.
authors.forename: B
authors.initials: B
authors.lastname: Alirezaie
authors.forename: R
authors.initials: R
authors.lastname: Shahbazi
authors.forename: S S
authors.initials: SS
authors.lastname: Safavieh
authors.forename: A
authors.initials: A
authors.lastname: Mohammad
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D014644:Genetic Variation; D016679:Genome, Viral; D006801:Humans; D008969:Molecular Sequence Data; D009107:Mumps / Q000517:prevention & control / Q000821:virology*; D009108:Mumps Vaccine / Q000235:genetics* / Q000276:immunology; D009109:Mumps virus / Q000235:genetics* / Q000276:immunology; D014613:Vaccines, Attenuated / Q000235:genetics / Q000276:immunology
chemical_list: D009108:Mumps Vaccine; D014613:Vaccines, Attenuated