title: Evaluation of the relative available energy of several dietary fiber preparations using breath hydrogen evolution in healthy humans.
abstract: A standardized simple, indirect method for assessing the relative energy of dietary fiber carbohydrates is not yet established. There is a need for a standardized in vivo assay. The objective of the present study was to evaluate the relative available energy (RAE) for 9 major dietary fiber materials (DFMs) based on fermentability from breath hydrogen excretion (BHE) in subjects. Fructooligosaccharide (FOS) was used as a reference. The study was conducted using a within-subject, repeated measures design and approved by the Ethical Committee of University of Nagasaki. After DFM ingestion, end-expiratory gas (750-mL) was collected at 1-h intervals for 8 h, as well as at 2-h intervals between 8 h and 14 h, and 30 min after waking up and 24 h after DFM ingestion. Breath hydrogen concentration was assessed with a gas chromatograph. The RAE of DFMs tested was evaluated based on the area under the curve (AUC) of BHE of FOS. Based on the ratio of AUC for 8 h, the RAE of polydextrose, partially hydrolysed guar gum, resistant maltodextrin and partially hydrolysed alginate was 1 kcal/g, and that of glucomannan, heat-moisture treatment and high-amylose cornstarch and cellulose was 0 kcal/g, while the RAE of all tested DEMs including cellulose and glucomannan was 1 kcal/g in the calculation based on AUCs for 14 h and 24 h in subjects. We suggest that a breath hydrogen collection period of 14 h or more could be used to measure RAE for a range of fiber preparations in vivo.
authors.affiliation: Graduate School of Human Health Science, University of Nagasaki, Siebold.
authors.forename: Tsuneyuki
authors.initials: T
authors.lastname: Oku
authors.forename: Sadako
authors.initials: S
authors.lastname: Nakamura
mesh_terms: D000328:Adult; D000464:Alginates / Q000378:metabolism; D000688:Amylose / Q000378:metabolism; D019540:Area Under Curve; D001944:Breath Tests; D002482:Cellulose / Q000378:metabolism; D002849:Chromatography, Gas; D004040:Dietary Carbohydrates / Q000378:metabolism*; D004043:Dietary Fiber / Q000378:metabolism*; D002149:Energy Intake; D005260:Female; D005285:Fermentation; D005685:Galactans / Q000378:metabolism; D005936:Glucans / Q000378:metabolism; D020723:Glucuronic Acid / Q000378:metabolism; D006603:Hexuronic Acids / Q000378:metabolism; D006801:Humans; D006859:Hydrogen / Q000378:metabolism*; D008351:Mannans / Q000378:metabolism; D009844:Oligosaccharides / Q000378:metabolism; D053149:Plant Gums / Q000378:metabolism; D011134:Polysaccharides / Q000378:metabolism*; D012016:Reference Values; D013213:Starch / Q000378:metabolism; D055815:Young Adult
chemical_list: D000464:Alginates; D004040:Dietary Carbohydrates; D004043:Dietary Fiber; D005685:Galactans; D005936:Glucans; D006603:Hexuronic Acids; D008351:Mannans; D009844:Oligosaccharides; D053149:Plant Gums; D011134:Polysaccharides; C116580:fructooligosaccharide; C022901:(1-6)-alpha-glucomannan; C008315:maltodextrin; D006859:Hydrogen; D020723:Glucuronic Acid; D002482:Cellulose; D013213:Starch; D000688:Amylose; C007894:guar gum; C033375:polydextrose