title: [G894T (NOS3) and G1958A (MTHFD1) gene polymorphisms and risk of ischemic heart disease in Yucatan, Mexico].
abstract: OBJECTIVE
  Cardiovascular medicine is focused on the search for genetic risk markers with predictive and/or prognostic value. Among the genetic variants of interest are G894T endothelial nitric oxide synthase and G1958A methylenetetrahydrofolate dehydrogenase1 gene polymorphisms. The aim of this study was to determine the possible association between these polymorphisms and ischemic heart disease in patients from Southern of Mexico (Yucatán).
  
  
  METHODS
  Case-control study matched by age, sex and origin was designed. We studied 98 patients with coronary disease and 101 controls. Participants were evaluated for the usual risk factors. The polymorphisms were identified using the polymerase chain reaction/restriction fragment length polymorphism analysis. Informed consent was obtained from all participants.
  
  
  RESULTS
  The G894T and G1958A polymorphisms were not associated with ischemic heart disease, however, the TT genotype (G894T) was associated with the angina (OR=10.2; 95%CI, 1.51-68.8; p=0.025). The genotype GT (G894T) was the most frequent in patients with family history of coronary artery disease. Multiple logistic regression analysis identified smoking (OR=5.21; 95%CI, 2.1-12.9; p=0.000), hypertension (OR=3.54; 95%CI, 1.47-8.56; p=0.005) and obesity (OR=1.16; 95%CI, 1.1-1.27; p=0.001) as risk factors predicting the ischemic heart disease.
  
  
  CONCLUSIONS
  The G894T and G1958A polymorphisms showed not association with ischemic heart disease. However, homozygosis for the 894T allele (NOS3) confers at risk to develop angina on Yucatán.
authors.affiliation: Laboratorio de Genética, Centro de Investigaciones Regionales «Dr. Hideyo Noguchi» (CIR), Universidad Autónoma de Yucatán, Mérida, México. Electronic address: igrid.garcia@uady.mx.
authors.forename: Igrid
authors.initials: I
authors.lastname: García-González
authors.affiliation: Consulta de Cardiología, Hospital Regional Mérida del ISSSTE, Mérida, México.
authors.forename: Alberto de Jesús
authors.initials: Ade J
authors.lastname: Solís-Cárdenas
authors.affiliation: Consulta de Cardiología, Hospital Regional Mérida del ISSSTE, Mérida, México.
authors.forename: Jorge A
authors.initials: JA
authors.lastname: Flores-Ocampo
authors.affiliation: Consulta de Cardiología, Hospital General «Dr. Agustín O'Horan», Mérida, México.
authors.forename: Ricardo
authors.initials: R
authors.lastname: Alejos-Mex
authors.affiliation: Unidad Cardiometabólica, Facultad de Medicina, Universidad Autónoma de Yucatán, Mérida, México.
authors.forename: Luis Fernando
authors.initials: LF
authors.lastname: Herrera-Sánchez
authors.affiliation: Laboratorio de Genética, Centro de Investigaciones Regionales «Dr. Hideyo Noguchi» (CIR), Universidad Autónoma de Yucatán, Mérida, México.
authors.forename: Lizbeth Josefina
authors.initials: LJ
authors.lastname: González-Herrera
mesh_terms: D000328:Adult; D000483:Alleles; D000619:Aminohydrolases / Q000235:genetics*; D000787:Angina Pectoris / Q000235:genetics* / Q000503:physiopathology; D016022:Case-Control Studies; D003327:Coronary Disease / Q000235:genetics / Q000503:physiopathology; D005260:Female; D005574:Formate-Tetrahydrofolate Ligase / Q000235:genetics*; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D016015:Logistic Models; D008297:Male; D008754:Methylenetetrahydrofolate Dehydrogenase (NADP) / Q000235:genetics*; D008800:Mexico / Q000453:epidemiology; D008875:Middle Aged; D009097:Multienzyme Complexes / Q000235:genetics*; D017202:Myocardial Ischemia / Q000235:genetics* / Q000503:physiopathology; D052250:Nitric Oxide Synthase Type III / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D012307:Risk Factors
chemical_list: D009097:Multienzyme Complexes; C021836:formyl-methenyl-methylenetetrahydrofolate synthetase; C496314:NOS3 protein, human; D052250:Nitric Oxide Synthase Type III; D008754:Methylenetetrahydrofolate Dehydrogenase (NADP); D000619:Aminohydrolases; D005574:Formate-Tetrahydrofolate Ligase
keywords: Cardiopatía isquémica; Genotipo; Genotype, Polymorphism, NOS3; Ischemic heart disease; MTHFD1; NOS3; Polimorfismo