title: Ultrasound-assisted combined with nano-sized molecularly imprinted polymer for selective extraction and pre-concentration of amitriptyline in human plasma with gas chromatography-flame detection.
abstract: A new process was developed for the selective extraction and pre-concentration of amitriptyline (AT) from human plasma using nano-sized molecularly imprinted polymer (MIP) with ultrasound-assisted extraction (UAE). The nano-sized AT imprinted polymer particles were synthesized using suspension polymerization in silicon oil and characterized by Fourier transform infrared (FT-IR) spectroscopy and scanning electron microscope (SEM) methods. With the application of optimized values, linearity values in the ranges of 20-200μgmL(-1) and 35-200μgmL(-1) were obtained for AT with the correlation of determination values (r(2)) 0.998 and 0.995 in water and plasma, respectively. The limits of detections (S/N=3) for AT were found to be 0.7 and 1.2μgmL(-1) in water and plasma, respectively. The enrichment factors of AT in water and plasma were 52 and 40, respectively. The inter-day precisions (%) were in the range of 5.8-9.2%. Relative recovery rates ranged from 82.4% to 92.3%. The method was successfully applied to determine AT in the human plasma samples.
authors.affiliation: Department of Chemistry, Mahabad Branch, Islamic Azad University, Mahabad, P.O. Box 443, Iran. Electronic address: khanahmadzadeh@iau-mahabad.ac.ir.
authors.forename: Salah
authors.initials: S
authors.lastname: Khanahmadzadeh
authors.affiliation: Department of Chemistry, Mahabad Branch, Islamic Azad University, Mahabad, P.O. Box 443, Iran.
authors.forename: Ahmad
authors.initials: A
authors.lastname: Tarigh
mesh_terms: D000639:Amitriptyline / Q000097:blood / Q000302:isolation & purification*; D000929:Antidepressive Agents, Tricyclic / Q000097:blood / Q000302:isolation & purification*; D002849:Chromatography, Gas / Q000379:methods*; D006801:Humans; D057230:Limit of Detection; D008855:Microscopy, Electron, Scanning; D054802:Molecular Imprinting; D053758:Nanoparticles; D011108:Polymers / Q000737:chemistry*; D052616:Solid Phase Extraction; D017550:Spectroscopy, Fourier Transform Infrared; D013696:Temperature
chemical_list: D000929:Antidepressive Agents, Tricyclic; D011108:Polymers; D000639:Amitriptyline
keywords: Amitriptyline; Gas chromatography-flame ionization; Nano-sized molecularly imprinted polymer; Ultrasound-assisted