title: Sorghum (Sorghum bicolor).
abstract: Agrobacterium-mediated transformation of sorghum (Sorghum bicolor L. Moench) targeting immature embryo explants is a route to introduce transgenic alleles into the crop. The protocol requires maintenance of quality stock plants under greenhouse conditions for a constant supply of immature embryo explants. This is typically carried out by a regular sowing of seeds, minimal use of pesticides, and monitoring of plants to document pollen dispersal and bagging of heads. The time frame from explant inoculation to establishment of a primary transgenic event in the greenhouse typically ranges from 4 to 6 months. Seed set in the primary transformants is comparable to greenhouse-grown stock plants, with the majority of the transgenic alleles being inherited as a single functional locus.
authors.affiliation: Department of Agronomy and Horticulture, Center for Plant Science Innovation, George W. Beadle Center, University of Nebraska-Lincoln, 1901 Vine Street, Lincoln, NE, 68588-0665, USA.
authors.forename: Xiaomei
authors.initials: X
authors.lastname: Guo
authors.forename: Zhengxiang
authors.initials: Z
authors.lastname: Ge
authors.forename: Shirley J
authors.initials: SJ
authors.lastname: Sato
authors.forename: Tom E
authors.initials: TE
authors.lastname: Clemente
mesh_terms: D000383:Agriculture / Q000379:methods; D016960:Agrobacterium tumefaciens / Q000235:genetics; D018920:Coculture Techniques; D005821:Genetic Techniques; D030821:Plants, Genetically Modified; D011058:Pollen / Q000235:genetics; D012639:Seeds / Q000235:genetics; D045868:Sorghum / Q000235:genetics* / Q000254:growth & development; D013242:Sterilization; D014169:Transformation, Bacterial