title: Predictive criteria to study the pathogenesis of malaria-associated ALI/ARDS in mice.
abstract: Malaria-associated acute lung injury/acute respiratory distress syndrome (ALI/ARDS) often results in morbidity and mortality. Murine models to study malaria-associated ALI/ARDS have been described; we still lack a method of distinguishing which mice will develop ALI/ARDS before death. This work aimed to characterize malaria-associated ALI/ARDS in a murine model and to demonstrate the first method to predict whether mice are suffering from ALI/ARDS before death. DBA/2 mice infected with Plasmodium berghei ANKA developing ALI/ARDS or hyperparasitemia (HP) were compared using histopathology, PaO2 measurement, pulmonary X-ray, breathing capacity, lung permeability, and serum vascular endothelial growth factor (VEGF) levels according to either the day of death or the suggested predictive criteria. We proposed a model to predict malaria-associated ALI/ARDS using breathing patterns (enhanced pause and frequency respiration) and parasitemia as predictive criteria from mice whose cause of death was known to retrospectively diagnose the sacrificed mice as likely to die of ALI/ARDS as early as 7 days after infection. Using this method, we showed increased VEGF levels and increased lung permeability in mice predicted to die of ALI/ARDS. This proposed method for accurately identifying mice suffering from ALI/ARDS before death will enable the use of this model to study the pathogenesis of this disease.
authors.affiliation: Departamento de Imunologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, Edifício Biomédicas IV, Avenida Professor Lineu Prestes, No. 1730, 05508-900 São Paulo, SP, Brazil ; Departamento de Ciências Biológicas, Universidade Federal de São Paulo, Rua Professor Artur Riedel, No. 275, Jardim Eldorado, 09972-270 Diadema, SP, Brazil.
authors.forename: Luana S
authors.initials: LS
authors.lastname: Ortolan
authors.affiliation: Instituto de Medicina Tropical de São Paulo, Universidade de São Paulo, Avenida Dr. Enéas Carvalho de Aguiar, No. 470, 05403-000 São Paulo, SP, Brazil.
authors.forename: Michelle K
authors.initials: MK
authors.lastname: Sercundes
authors.affiliation: Departamento de Ciências Exatas e da Terra, Universidade Federal de São Paulo, Rua Professor Artur Riedel, No. 275, Jardim Eldorado, 09972-270 Diadema, SP, Brazil.
authors.forename: Renato
authors.identifier: 0000-0002-6441-7513
authors.initials: R
authors.lastname: Barboza
authors.affiliation: Instituto de Medicina Tropical de São Paulo, Universidade de São Paulo, Avenida Dr. Enéas Carvalho de Aguiar, No. 470, 05403-000 São Paulo, SP, Brazil.
authors.forename: Daniela
authors.initials: D
authors.lastname: Debone
authors.affiliation: Departamento de Parasitologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, Avenida Professor Lineu Prestes, No. 1374, Edifício Biomédicas II Cidade Universitária "Armando Salles Oliveira", 05508-000 São Paulo, SP, Brazil.
authors.forename: Oscar
authors.identifier: 0000-0003-1204-7984
authors.initials: O
authors.lastname: Murillo
authors.affiliation: Departamento de Cirurgia, Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo, Avenida Professor Dr. Orlando Marques de Paiva, No. 87, Cidade Universitária, 05508 270 São Paulo, SP, Brazil.
authors.forename: Stefano C F
authors.initials: SC
authors.lastname: Hagen
authors.affiliation: Departamento de Imunologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, Edifício Biomédicas IV, Avenida Professor Lineu Prestes, No. 1730, 05508-900 São Paulo, SP, Brazil.
authors.forename: Momtchilo
authors.identifier: 0000-0003-3553-3184
authors.initials: M
authors.lastname: Russo
authors.affiliation: Departamento de Imunologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, Edifício Biomédicas IV, Avenida Professor Lineu Prestes, No. 1730, 05508-900 São Paulo, SP, Brazil.
authors.forename: Maria Regina
authors.initials: MR
authors.lastname: D' Império Lima
authors.affiliation: Departamento de Imunologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, Edifício Biomédicas IV, Avenida Professor Lineu Prestes, No. 1730, 05508-900 São Paulo, SP, Brazil.
authors.forename: José M
authors.initials: JM
authors.lastname: Alvarez
authors.affiliation: Departamento de Medicina Veterinária Preventiva e Saúde Animal, Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo, Avenida Professor Dr. Orlando Marques de Paiva, No. 87, Cidade Universitária, 05508 270 São Paulo, SP, Brazil.
authors.forename: Marcos
authors.identifier: 0000-0003-4752-6774
authors.initials: M
authors.lastname: Amaku
authors.affiliation: Departamento de Parasitologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, Avenida Professor Lineu Prestes, No. 1374, Edifício Biomédicas II Cidade Universitária "Armando Salles Oliveira", 05508-000 São Paulo, SP, Brazil.
authors.forename: Claudio R F
authors.initials: CR
authors.lastname: Marinho
authors.affiliation: Departamento de Imunologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, Edifício Biomédicas IV, Avenida Professor Lineu Prestes, No. 1730, 05508-900 São Paulo, SP, Brazil ; Departamento de Análises Clínicas e Toxicológicas, Faculdade de Ciências Farmacêuticas, Universidade de São Paulo, Avenida Professor Lineu Prestes, No. 580, Bloco 17, Cidade Universitária "Armando Salles Oliveira", 05508-000 São Paulo, SP, Brazil.
authors.forename: Sabrina
authors.initials: S
authors.lastname: Epiphanio
mesh_terms: D000818:Animals; D001831:Body Temperature; D004195:Disease Models, Animal; D008288:Malaria / Q000150:complications*; D008297:Male; D051379:Mice; D008811:Mice, Inbred DBA; D012128:Respiratory Distress Syndrome / Q000209:etiology / Q000378:metabolism / Q000473:pathology*; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism
chemical_list: D042461:Vascular Endothelial Growth Factor A