title: Analyzing the biosensor signal in flows: studies with glucose optrodes.
abstract: Responses of enzymatic bio-optrodes in flow regime were studied and an original model was proposed with the aim of establishing a reliable method for a quick determination of biosensor signal parameters, applicable for biosensor calibration. A dual-optrode glucose biosensor, comprising of a glucose bio-optrode and a reference oxygen optrode, both placed into identical flow channels, was developed and used as a model system. The signal parameters of this biosensor at different substrate concentrations were not dependent on the speed of the probe flow and could be determined from the initial part of the biosensor transient phase signal, providing a valuable tool for rapid analysis. In addition, the model helped to design the biosensor system with reduced impact of enzyme inactivation to the system stability (20% decrease of the enzyme activity lead to only a 1% decrease of the slope of the calibration curve) and hence significantly prolong the effective lifetime of bio-optrodes.
authors.affiliation: Institute of Chemistry, University of Tartu, Ravila 14a, Tartu 50411, Estonia.
authors.forename: K
authors.initials: K
authors.lastname: Kivirand
authors.affiliation: Institute of Physics, University of Tartu, Ravila 14c, Tartu 50411, Estonia.
authors.forename: A
authors.initials: A
authors.lastname: Floren
authors.affiliation: Institute of Chemistry, University of Tartu, Ravila 14a, Tartu 50411, Estonia.
authors.forename: M
authors.initials: M
authors.lastname: Kagan
authors.affiliation: Institute of Physics, University of Tartu, Ravila 14c, Tartu 50411, Estonia.
authors.forename: T
authors.initials: T
authors.lastname: Avarmaa
authors.affiliation: Institute of Chemistry, University of Tartu, Ravila 14a, Tartu 50411, Estonia.
authors.forename: T
authors.initials: T
authors.lastname: Rinken
authors.affiliation: Institute of Physics, University of Tartu, Ravila 14c, Tartu 50411, Estonia. Electronic address: raivo.jaaniso@ut.ee.
authors.forename: R
authors.initials: R
authors.lastname: Jaaniso
mesh_terms: D055162:Biocatalysis; D015374:Biosensing Techniques; D002138:Calibration; D005336:Fiber Optic Technology; D005947:Glucose / Q000032:analysis* / Q000378:metabolism; D005949:Glucose Oxidase / Q000378:metabolism*; D008962:Models, Theoretical; D010100:Oxygen / Q000378:metabolism*
chemical_list: D005949:Glucose Oxidase; D005947:Glucose; D010100:Oxygen
keywords: Dual-optrode biosensor; Flow regime; Glucose; Modeling; Pre-steady state calibration