title: Stress induces pain transition by potentiation of AMPA receptor phosphorylation.
abstract: Chronic postsurgical pain is a serious issue in clinical practice. After surgery, patients experience ongoing pain or become sensitive to incident, normally nonpainful stimulation. The intensity and duration of postsurgical pain vary. However, it is unclear how the transition from acute to chronic pain occurs. Here we showed that social defeat stress enhanced plantar incision-induced AMPA receptor GluA1 phosphorylation at the Ser831 site in the spinal cord and greatly prolonged plantar incision-induced pain. Interestingly, targeted mutation of the GluA1 phosphorylation site Ser831 significantly inhibited stress-induced prolongation of incisional pain. In addition, stress hormones enhanced GluA1 phosphorylation and AMPA receptor-mediated electrical activity in the spinal cord. Subthreshold stimulation induced spinal long-term potentiation in GluA1 phosphomimetic mutant mice, but not in wild-type mice. Therefore, spinal AMPA receptor phosphorylation contributes to the mechanisms underlying stress-induced pain transition.
authors.affiliation: Department of Anesthesiology and Critical Care Medicine, Basic Medical College, Zhengzhou University, Zhengzhou, Henan 450001, People's Republic of China.
authors.forename: Changsheng
authors.initials: C
authors.lastname: Li
authors.affiliation: Department of Anesthesiology and Critical Care Medicine, The Russell H. Morgan Department of Radiology and Radiological Science.
authors.forename: Ya
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Department of Anesthesiology and Critical Care Medicine, Basic Medical College, Zhengzhou University, Zhengzhou, Henan 450001, People's Republic of China.
authors.forename: Sufang
authors.identifier: http://orcid.org/0000-0003-2677-1295
authors.initials: S
authors.lastname: Liu
authors.affiliation: Solomon H. Snyder Department of Neuroscience, and.
authors.forename: Huaqiang
authors.initials: H
authors.lastname: Fang
authors.affiliation: Department of Anesthesiology and Critical Care Medicine.
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Anesthesiology and Critical Care Medicine.
authors.forename: Orion
authors.identifier: http://orcid.org/0000-0001-5451-0223
authors.initials: O
authors.lastname: Furmanski
authors.affiliation: Department of Anesthesiology and Critical Care Medicine.
authors.forename: John
authors.initials: J
authors.lastname: Skinner
authors.affiliation: Basic Medical College, Zhengzhou University, Zhengzhou, Henan 450001, People's Republic of China, Basic Medical College, Xinxiang Medical University, Xinxiang, Henan 453003, People's Republic of China, and.
authors.forename: Ying
authors.initials: Y
authors.lastname: Xing
authors.affiliation: Department of Anesthesiology and Critical Care Medicine.
authors.forename: Roger A
authors.initials: RA
authors.lastname: Johns
authors.affiliation: Solomon H. Snyder Department of Neuroscience, and Howard Hughes Medical Institute, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, tao@bcd.tamhsc.edu rhuganir@jhmi.edu.
authors.forename: Richard L
authors.initials: RL
authors.lastname: Huganir
authors.affiliation: Department of Anesthesiology and Critical Care Medicine, Department of Biomedical Sciences, Texas A&M University Baylor College of Dentistry, Dallas, Texas 75246 tao@bcd.tamhsc.edu rhuganir@jhmi.edu.
authors.forename: Feng
authors.identifier: http://orcid.org/0000-0001-5414-4542
authors.initials: F
authors.lastname: Tao
mesh_terms: D000818:Animals; D001640:Bicuculline / Q000494:pharmacology; D019921:Biotinylation; D018756:GABA Antagonists / Q000494:pharmacology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D010146:Pain / Q000503:physiopathology* / Q000523:psychology; D010147:Pain Measurement / Q000379:methods; D010766:Phosphorylation; D018091:Receptors, AMPA / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D012930:Social Dominance; D013315:Stress, Psychological / Q000503:physiopathology* / Q000523:psychology; D013569:Synapses / Q000502:physiology
chemical_list: D001640:Bicuculline; D018756:GABA Antagonists; D018091:Receptors, AMPA
keywords: AMPA receptor phosphorylation; pain transition; stress