title: [Antitumor enzyme L-lyzine-alpha-oxidase from Trichoderma harzianum Rifai F-180 and investigation of its action on L-lysine oxidation by capillar electrophoresis].
abstract: Trichoderma harzianum Rifai F-180, an organism producing the antitumor enzyme L-lysine-alpha-oxidase was cultivated and the enzyme was isolated and purified under the manufacturing conditions. The effect of L-lysine-alpha-oxidase on oxidation of L-lysine was investigated for the first time by capillary electrophoresis and the procedure conditions were developed. The reaction of L-lysine oxidative deamination is described and location of the reaction components picks on the elecrophoregrams was identified. The average rate of the catalytic reaction of L-lysine oxidation equal to 0.46 RU/min (7.7 x 10(-3) RU/sec) was determined. The use of the antitumor enzyme L-lysine-alpha-oxidase is recommended as a drug for the treatment of superficial tumors and tissue relative oxygen excess.
authors.forename: I P
authors.initials: IP
authors.lastname: Smirnova
authors.forename: V M
authors.initials: VM
authors.lastname: Shkinev
authors.forename: A V
authors.initials: AV
authors.lastname: Rudnev
authors.forename: O M
authors.initials: OM
authors.lastname: Kuznetsova
authors.forename: E V
authors.initials: EV
authors.lastname: Karimova
authors.forename: V S
authors.initials: VS
authors.lastname: Orlova
mesh_terms: D000594:Amino Acid Oxidoreductases / Q000737:chemistry* / Q000302:isolation & purification; D000970:Antineoplastic Agents / Q000737:chemistry* / Q000302:isolation & purification; D019075:Electrophoresis, Capillary; D004591:Electrophoresis, Polyacrylamide Gel; D057075:Enzyme Assays; D005656:Fungal Proteins / Q000737:chemistry* / Q000302:isolation & purification; D007700:Kinetics; D008239:Lysine / Q000737:chemistry*; D010084:Oxidation-Reduction; D014242:Trichoderma / Q000737:chemistry* / Q000201:enzymology
chemical_list: D000970:Antineoplastic Agents; D005656:Fungal Proteins; D000594:Amino Acid Oxidoreductases; C041123:L-lysine oxidase; D008239:Lysine