title: Long D13S317 variant allele: a cautionary case report.
abstract: Exclusion at locus D13S317 between a father and child was observed in a kinship case, which at first glance appeared as a silent allele. However, a closer inspection using three commercial kits showed that the observed pattern is due to a long variant allele overlapping with different loci in different kits. Sequencing of the variant revealed a duplication within D13S317, that had also created an additional binding site for short amplicon reverse primer. If ignored, genotyping results including this variant may be wrongly interpreted.
authors.affiliation: Laboratory of Forensic Biology, Department of Forensic Medicine, Hjelt Institute, P.O. Box 40, FI-00014 University of Helsinki, Finland.
authors.forename: Minttu
authors.initials: M
authors.lastname: Hedman
authors.affiliation: Laboratory of Forensic Biology, Department of Forensic Medicine, Hjelt Institute, P.O. Box 40, FI-00014 University of Helsinki, Finland. Electronic address: jukka.palo@helsinki.fi.
authors.forename: Jukka U
authors.initials: JU
authors.lastname: Palo
mesh_terms: D000483:Alleles; D001483:Base Sequence; D005260:Female; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data
keywords: D13S317; Human identification; Variant allele