title: Quercetin conjugated superparamagnetic magnetite nanoparticles for in-vitro analysis of breast cancer cell lines for chemotherapy applications.
abstract: The magnetic nanoparticles attract increasing interest due to their opportunities in cancer therapy and used as drug carriers for several other diseases. The present study investigates the quercetin conjugated superparamagnetic Fe3O4 nanoparticles for in-vitro analysis of breast cancer cell lines for chemotherapy. A simple precipitation method was used to prepare the dextran coated Fe3O4 nanoparticles and the anticancer flavonoid quercetin was conjugated on the surface via carboxylic/amine group using nanoprecipitation method. The structural, morphological and the magnetic properties of the prepared materials were studied by using X-ray diffractometer (XRD), Fourier transformed infer-red spectrometer (FTIR), transmission electron microscope (TEM) and vibrating sample magnetometer (VSM). The MTT (3-(4,5-dimethylthiahiazol-2-yl)-2,5-diphenyl tetrazolium) assay of dextran coated Fe3O4 nanoparticles did not exhibit notable toxicity against MCF7 cells, whereas the cytotoxicity of quercetin conjugated Fe3O4 nanoparticles increased significantly in comparison with pure quercetin. The incubation of MCF-7 cells with quercetin conjugated Fe3O4 nanoparticles (QCMNPs) shows significant changes in cellular morphology observed through fluorescent microscopy. The results validate the prepared quercetin conjugated Fe3O4 nanoparticles are promising anticancer agents for targeted drug delivery.
authors.affiliation: Department of Nanoscience and Technology, Bharathiar University, Coimbatore 641046, India.
authors.forename: S Rajesh
authors.initials: SR
authors.lastname: Kumar
authors.affiliation: Department of Nanoscience and Technology, Bharathiar University, Coimbatore 641046, India.
authors.forename: S
authors.initials: S
authors.lastname: Priyatharshni
authors.affiliation: Proteomics and Molecular Cell Physiology Lab, Department of Zoology, Bharathiar University, Coimbatore 641046, India.
authors.forename: V N
authors.initials: VN
authors.lastname: Babu
authors.affiliation: Department of Nanoscience and Technology, Bharathiar University, Coimbatore 641046, India.
authors.forename: D
authors.initials: D
authors.lastname: Mangalaraj
authors.affiliation: Department of Nanoscience and Technology, Bharathiar University, Coimbatore 641046, India.
authors.forename: C
authors.initials: C
authors.lastname: Viswanathan
authors.affiliation: Proteomics and Molecular Cell Physiology Lab, Department of Zoology, Bharathiar University, Coimbatore 641046, India.
authors.forename: S
authors.initials: S
authors.lastname: Kannan
authors.affiliation: Department of Nanoscience and Technology, Bharathiar University, Coimbatore 641046, India. Electronic address: ponpandian@buc.edu.in.
authors.forename: N
authors.initials: N
authors.lastname: Ponpandian
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry* / Q000627:therapeutic use; D005260:Female; D052203:Ferrosoferric Oxide / Q000737:chemistry*; D006801:Humans; D061986:MCF-7 Cells; D008280:Magnetics; D046529:Microscopy, Electron, Transmission; D008856:Microscopy, Fluorescence; D053758:Nanoparticles / Q000737:chemistry*; D011794:Quercetin / Q000737:chemistry* / Q000627:therapeutic use; D013499:Surface Properties; D014961:X-Ray Diffraction
chemical_list: D000970:Antineoplastic Agents; D011794:Quercetin; D052203:Ferrosoferric Oxide
keywords: Anti-cancer; Drug releasing; Magnetite; Nanoprism; Quercetin