title: Novel intein-containing DNA specific primers for rapid identification of Candida glabrata using Real-Time PCR assays.
abstract: Candida glabrata is an opportunistic human pathogen known to cause systemic and vaginal candidiasis. Rapid detection of Candida glabrata is indispensable for appropriate selection of antifungal drugs for chemotherapy. The study describes a unique intein-containing DNA fragment for specific detection of C. glabrata. The designed oligonucleotides detected C. glabrata (Ct mean: 24.75 ± 1.1 and Tm: 70.08 ± 0.23°C) in Real-Time PCR assays. The fluorescent signals were negative when the primers were tested for cross-species and cross-genera amplifications. In conclusion, our study recommends a novel primer set for developing a quick identification system which does not require laborious and time-consuming experimentations.
authors.affiliation: Department of Microbiology, PRIST University, Thanjavur 614 904, Tamil Nadu, India.
authors.forename: R Satish
authors.initials: RS
authors.lastname: Kumar
authors.affiliation: Department of Microbiology, PRIST University, Thanjavur 614 904, Tamil Nadu, India. Electronic address: marineramesh2020@gmail.com.
authors.forename: S
authors.initials: S
authors.lastname: Ramesh
mesh_terms: D001483:Base Sequence; D041221:Candida glabrata / Q000235:genetics* / Q000302:isolation & purification; D002177:Candidiasis / Q000175:diagnosis* / Q000235:genetics / Q000382:microbiology; D017931:DNA Primers / Q000737:chemistry / Q000235:genetics; D004271:DNA, Fungal / Q000032:analysis / Q000235:genetics / Q000302:isolation & purification; D005260:Female; D006801:Humans; D047668:Inteins; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D013045:Species Specificity
chemical_list: D017931:DNA Primers; D004271:DNA, Fungal
keywords: C. glabrata; Détection rapide; Intein; Intéine; PCR en temps réel; Rapid detection; Real-Time PCR