title: LncRNA-HOST2 regulates cell biological behaviors in epithelial ovarian cancer through a mechanism involving microRNA let-7b.
abstract: Recently, a large number of long non-coding RNAs (lncRNAs) have been reported in mammalian genomes and are evolutionarily conserved and presumably function in many biological events, especially in the pathogenesis of diverse human cancers. A lncRNA, named HOST2 (human ovarian cancer-specific transcript 2), was once reported to specifically be expressed at high level in human ovarian cancer. However, how HOST2 acts to regulate gene functions in ovarian carcinogenesis has remained enigmatic. Here we report, for the first time, that HOST2 promotes tumor cell migration, invasion and proliferation in epithelial ovarian cancer by working in key aspects of biological behaviors. In the present study, bioinformatics analysis indicated that HOST2 binds with microRNA let-7b, a potent tumor suppressor, which was then verified to target HOST2. Our results showed that HOST2 harbors a let-7b binding site and modulates let-7b availability by acting as a molecular sponge. HOST2 inhibits let-7b functions, which post-transcriptionally suppress the expression of targets, including some oncogenes that regulate cell growth and motility. Additionally, understanding HOST2/let-7b-dependent regulation may lead to alternative approaches for the diagnosis and cure of this deadly disease.
authors.affiliation: Chinese PLA General Hospital, Beijing 100853, China Changhai Hospital of Second Military Medical University, Shanghai 200433, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Gao
authors.affiliation: Air Force General Hospital, Beijing 100142, China and Beijing Military Region General Hospital, Beijing 100700, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Meng
authors.affiliation: Changhai Hospital of Second Military Medical University, Shanghai 200433, China.
authors.forename: Shupeng
authors.initials: S
authors.lastname: Liu
authors.affiliation: Changhai Hospital of Second Military Medical University, Shanghai 200433, China.
authors.forename: Jingjing
authors.initials: J
authors.lastname: Hu
authors.affiliation: Changhai Hospital of Second Military Medical University, Shanghai 200433, China.
authors.forename: Yemin
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Changhai Hospital of Second Military Medical University, Shanghai 200433, China.
authors.forename: Tingting
authors.initials: T
authors.lastname: Jiao
authors.affiliation: Changhai Hospital of Second Military Medical University, Shanghai 200433, China.
authors.forename: Yujie
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Changhai Hospital of Second Military Medical University, Shanghai 200433, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Ou
authors.affiliation: Changhai Hospital of Second Military Medical University, Shanghai 200433, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Wang
authors.affiliation: Changhai Hospital of Second Military Medical University, Shanghai 200433, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Yao
authors.affiliation: Changhai Hospital of Second Military Medical University, Shanghai 200433, China.
authors.forename: Shanrong
authors.initials: S
authors.lastname: Liu
authors.affiliation: Changhai Hospital of Second Military Medical University, Shanghai 200433, China huining324@aliyun.com.
authors.forename: Ning
authors.initials: N
authors.lastname: Hui
mesh_terms: D000818:Animals; D001665:Binding Sites; D000077216:Carcinoma, Ovarian Epithelial; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015972:Gene Expression Regulation, Neoplastic; D006367:HeLa Cells; D056945:Hep G2 Cells; D006801:Humans; D061986:MCF-7 Cells; D051379:Mice; D035683:MicroRNAs / Q000378:metabolism*; D009361:Neoplasm Invasiveness; D009368:Neoplasm Transplantation; D009375:Neoplasms, Glandular and Epithelial / Q000378:metabolism* / Q000473:pathology*; D010051:Ovarian Neoplasms / Q000378:metabolism* / Q000473:pathology*; D062085:RNA, Long Noncoding / Q000737:chemistry / Q000378:metabolism*; D015398:Signal Transduction
chemical_list: C000599552:LncRNA-HOST2, human; D035683:MicroRNAs; D062085:RNA, Long Noncoding; C487931:mirnlet7 microRNA, human