title: Protein expression differences between lung adenocarcinoma and squamous cell carcinoma with brain metastasis.
abstract: OBJECTIVE
  We investigated tissue biomarkers in non-small cell lung cancer (NSCLC) to find indicators of brain metastasis and peritumoral brain edema.
  
  
  METHODS
  Fifty-two cases were studied out of which 26 had corresponding brain metastatic tissue. Clinicopathological characteristics of tumors were correlated with biomarkers of cell adhesion, cell growth, cell cycle and apoptosis regulation that were previously immunohistochemically studied but never analyzed separately according to histological subgroups, gender and smoking history.
  
  
  RESULTS
  Increased collagen XVII in adenocarcinoma (ADC) and increased caspase-9, CD44v6, and decreased cellular apoptosis susceptibility protein (CAS) and Ki-67 in squamous cell carcinoma (SCC) correlated significantly with brain metastasis. Increased β-catenin, E-cadherin and decreased caspase-9 expression in primary SCC, and decreased CD44v6 expression in brain metastatic SCC tissues showed a significant correlation with the extent of peritumoral brain edema. Positive correlation between smoking and biomarker expression could be observed in metastatic ADCs with p16 and caspase-8, while-negative correlation was found in SCC without brain metastasis with caspase-3, and in SCC with brain metastasis with p27.
  
  
  CONCLUSIONS
  Our results highlight the importance of separate analysis of biomarker expression in histological subtypes of NSCLC.
authors.affiliation: Department of Pulmonology, Semmelweis University, Budapest, Hungary.
authors.forename: Katalin
authors.initials: K
authors.lastname: Fábián
authors.affiliation: 2nd Department of Pathology, Semmelweis University, Budapest, Hungary.
authors.forename: Zsuzsanna
authors.initials: Z
authors.lastname: Németh
authors.affiliation: Department of Surgery, University of Szeged, Szeged, Hungary.
authors.forename: József
authors.initials: J
authors.lastname: Furák
authors.affiliation: Department of Pathology, University of Szeged, Szeged, Hungary.
authors.forename: László
authors.initials: L
authors.lastname: Tiszlavicz
authors.affiliation: 1st Department of Pathology and Experimental Cancer Research, Semmelweis University of Medicine, Budapest, Hungary.
authors.forename: Judit
authors.initials: J
authors.lastname: Pápay
authors.affiliation: 1st Department of Pathology and Experimental Cancer Research, Semmelweis University of Medicine, Budapest, Hungary.
authors.forename: Tibor
authors.initials: T
authors.lastname: Krenács
authors.affiliation: 2nd Department of Pathology, Semmelweis University, Budapest, Hungary.
authors.forename: József
authors.initials: J
authors.lastname: Tímár
authors.affiliation: Department of Pulmonology, Semmelweis University, Budapest, Hungary drmoldvay@hotmail.com.
authors.forename: Judit
authors.initials: J
authors.lastname: Moldvay
mesh_terms: D000230:Adenocarcinoma / Q000378:metabolism* / Q000473:pathology*; D000328:Adult; D000368:Aged; D015415:Biomarkers; D001932:Brain Neoplasms / Q000556:secondary*; D002294:Carcinoma, Squamous Cell / Q000378:metabolism* / Q000473:pathology*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008175:Lung Neoplasms / Q000378:metabolism* / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D011379:Prognosis; D040901:Proteomics; D012307:Risk Factors
chemical_list: D015415:Biomarkers
keywords: CD44v6; Lung cancer; brain metastasis; caspase; gender differences; immunohistochemistry; peritumoral edema; smoking