title: Two missense mutations in exon 9 of caprine PRLR gene were associated with litter size.
abstract: Guanzhong (n = 321) and Boer (n = 191) goat breeds were used to detect single nucleotide polymorphisms (SNPs) in the coding regions of the prolactin receptor (PRLR) gene by DNA sequencing and PCR-RFLP. Two SNPs (c.1457G>A and c.1645G>A) were identified that caused amino acid variations p.Ser485Asn and p.Val548Met respectively. Statistical results indicated that the c.1457G>A and c.1645G>A SNPs were significantly associated with litter size in Boer and Guanzhong goat breeds. Further analysis revealed that combined genotype C4 (GGGG) and haplotype G-G were better than the others for litter size in both goat breeds. These results might contribute to goat genetic resources and breeding.
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, 712100, China.
authors.forename: J X
authors.initials: JX
authors.lastname: Hou
authors.forename: X P
authors.initials: XP
authors.lastname: An
authors.forename: P
authors.initials: P
authors.lastname: Han
authors.forename: J Y
authors.initials: JY
authors.lastname: Peng
authors.forename: B Y
authors.initials: BY
authors.lastname: Cao
mesh_terms: D000818:Animals; D001947:Breeding; D005091:Exons; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D006041:Goats / Q000235:genetics*; D006239:Haplotypes; D008098:Litter Size / Q000235:genetics*; D008969:Molecular Sequence Data; D020125:Mutation, Missense; D020641:Polymorphism, Single Nucleotide; D019655:Quantitative Trait, Heritable; D011981:Receptors, Prolactin / Q000235:genetics*; D017422:Sequence Analysis, DNA
chemical_list: D011981:Receptors, Prolactin
keywords: PCR-RFLP; SNP; breeding; genotype