title: A nucleoprotein-enriched diet suppresses dopaminergic neuronal cell loss and motor deficit in mice with MPTP-induced Parkinson's disease.
abstract: Parkinson's disease (PD) is an obstinate progressive neurodegenerative disease and characterized by locomotor impairment and dopaminergic neuronal degeneration in the substantia nigra pars compacta (SNc). We examined in here the dietary effect of nucleoprotein (NP) extracted from salmon soft roe on 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-injected PD-like mice model to prevent the symptom as an alternative medicine. Male C57/BL6 mice were given either an artificially modified NP-free diet (NF) or NF supplied with 1.2% NP for 1 week. Then, mice were injected intraperitoneally four times with 20 mg/kg MPTP. Seven days later, locomotor activity was examined, and the brains were immunostained with tyrosine hydroxylase (TH) and Iba1 antibodies. Moreover, in situ detection of superoxide anion (O2(-)) and gene expression of mitochondrial electron transfer chain gene, Cox8b was evaluated in midbrains. NP-fed animals showed significantly reduced locomotor impairment and an increased number of TH-positive cells in the SNc compared with NF animals. The NP-fed animals also showed reduced lower levels of O2(-) and up-regulation of Cox8b levels and Iba1 immunoreactivity, suggesting that inflammation and oxidative stress were suppressed and mitochondrial impairment was relieved in these animals. Supplementation of the diet with NP may serve as a useful preventive measure to slow the onset of PD.
authors.affiliation: Department of Anatomy, Showa University School of Medicine, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo, 142-8555, Japan.
authors.forename: Keisuke
authors.initials: K
authors.lastname: Kiriyama
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Ohtaki
authors.forename: Natsuki
authors.initials: N
authors.lastname: Kobayashi
authors.forename: Norimitsu
authors.initials: N
authors.lastname: Murai
authors.forename: Minako
authors.initials: M
authors.lastname: Matsumoto
authors.forename: Shun
authors.initials: S
authors.lastname: Sasaki
authors.forename: Chika
authors.initials: C
authors.lastname: Sawa
authors.forename: Kazue
authors.initials: K
authors.lastname: Satoh
authors.forename: Masaji
authors.initials: M
authors.lastname: Matsunaga
authors.forename: Seiji
authors.initials: S
authors.lastname: Shioda
mesh_terms: D000818:Animals; D019587:Dietary Supplements; D059290:Dopaminergic Neurons / Q000187:drug effects* / Q000378:metabolism; D003576:Electron Transport Complex IV / Q000235:genetics / Q000378:metabolism; D008124:Locomotion; D020267:MPTP Poisoning / Q000188:drug therapy* / Q000517:prevention & control; D008297:Male; D008636:Mesencephalon / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D009698:Nucleoproteins / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use*; D013481:Superoxides / Q000378:metabolism; D014446:Tyrosine 3-Monooxygenase / Q000235:genetics / Q000378:metabolism
chemical_list: D009698:Nucleoproteins; D013481:Superoxides; D014446:Tyrosine 3-Monooxygenase; D003576:Electron Transport Complex IV