title: Engineered yeast for enhanced CO2mineralization.
abstract: In this work, a biologically catalyzed CO2mineralization process for the capture of CO2from point sources was designed, constructed at a laboratory scale, and, using standard chemical process scale-up protocols, was modeled and evaluated at an industrial scale. A yeast display system inSaccharomyces cerevisaewas used to screen several carbonic anhydrase isoforms and mineralization peptides for their impact on CO2hydration, CaCO3mineralization, and particle settling rate. Enhanced rates for each of these steps in the CaCO3mineralization process were confirmed using quantitative techniques in lab-scale measurements. The effect of these enhanced rates on the CO2capture cost in an industrial scale CO2mineralization process using coal fly ash as the CaO source was evaluated. The model predicts a process using bCA2- yeast and fly ash is ~10% more cost effective per ton of CO2captured than a process with no biological molecules, a savings not realized by wild-type yeast and high-temperature stable recombinant CA2 alone or in combination. The levelized cost of electricity for a power plant using this process was calculated and scenarios in which this process compares favorably to CO2capture by MEA absorption process are presented.
authors.affiliation: Department of Biological Engineering, The David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, 32 Vassar Street, Building 76-561, Cambridge, MA 02142, USA. Tel: +1 617 324 3400.
authors.forename: Roberto
authors.initials: R
authors.lastname: Barbero
authors.affiliation: Eni s.p.a - Research Center for Non-Conventional Energy, Istituto Eni Donegani, Via Fauser, 4 - 28100 Novara (NO), Italy. ; Tel: +39 032 144 7614.
authors.forename: Lino
authors.initials: L
authors.lastname: Carnelli
authors.affiliation: UC Santa Barbara, Santa Barbara, CA, USA. ; Tel: +1 805 893 5845.
authors.forename: Anna
authors.initials: A
authors.lastname: Simon
authors.affiliation: Massachusetts Institute of Technology, 400 Memorial Drive, Cambridge, MA, USA. Tel: +1 603 770 8225.
authors.forename: Albert
authors.initials: A
authors.lastname: Kao
authors.affiliation: Eni s.p.a - Research Center for Non-Conventional Energy, Istituto Eni Donegani, Via Fauser, 4 - 28100 Novara (NO), Italy. ; Tel: +39 032 144 7476.
authors.forename: Alessandra d'Arminio
authors.initials: AD
authors.lastname: Monforte
authors.affiliation: Eni s.p.a - Research Center for Non-Conventional Energy, Istituto Eni Donegani, Via Fauser, 4 - 28100 Novara (NO), Italy. ; Tel: +39 032 144 7484.
authors.forename: Moreno
authors.initials: M
authors.lastname: Riccò
authors.affiliation: Eni s.p.a - Research and Technology Innovation, Via Fauser, 4 - 28100 Novara (NO), Italy. Tel: +39 0321 447655.
authors.forename: Daniele
authors.initials: D
authors.lastname: Bianchi
authors.affiliation: Department of Materials Science and Engineering and Department of Biological Engineering, The David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, 32 Vassar Street, Building 76-561, Cambridge, MA 02142, USA. ; Tel: +1 617 324 2800.
authors.forename: Angela
authors.initials: A
authors.lastname: Belcher