title: DNA binding studies of hematoxylin-Dy(ш) complex by spectrometry using acridine orange as a probe.
abstract: The interaction of a hematoxylin(HE)-Dy(Ш) complex with herring sperm DNA(hsDNA) was studied using acridine orange(AO) as a probe by UV-vis absorption, circular dichroism(CD), fluorescence spectroscopy and viscosity measurements. From the results of the probe experiment, we found that the HE-Dy(Ш) complex could compete with AO for intercalating into hsDNA. The binding constants of the HE-Dy(Ш) complex to hsDNA was obtained by the double reciprocal method and indicated that the affinity between hsDNA and the complex is weaker than that between hsDNA and classical intercalators. The thermodynamic parameters(ΔH°, ΔG°, ΔS°) were calculated from the UV-vis absorption data measured at two different temperatures. Further experimental results suggested that there exist groove binding and partial intercalation binding between hsDNA and HE-Dy(Ш) complex.
authors.affiliation: a College of Chemistry and Materials Science , Sichuan Normal University , Chengdu , P.R.China.
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Xiong
authors.forename: Jianhang
authors.initials: J
authors.lastname: Huang
authors.forename: Xingming
authors.initials: X
authors.lastname: Wang
mesh_terms: D000165:Acridine Orange; D000818:Animals; D002942:Circular Dichroism; D056831:Coordination Complexes / Q000737:chemistry; D004247:DNA / Q000737:chemistry*; D004419:Dysprosium / Q000737:chemistry*; D005399:Fishes; D006416:Hematoxylin / Q000737:chemistry*; D057927:Hydrophobic and Hydrophilic Interactions; D007364:Intercalating Agents / Q000737:chemistry; D008297:Male; D013050:Spectrometry, Fluorescence; D013057:Spectrum Analysis; D013094:Spermatozoa / Q000737:chemistry; D013816:Thermodynamics; D014783:Viscosity
chemical_list: D056831:Coordination Complexes; D007364:Intercalating Agents; D004419:Dysprosium; D004247:DNA; D000165:Acridine Orange; D006416:Hematoxylin
keywords: DNA; Dy(III); acridine orange; hematoxylin; spectrometry