title: miR-185-3p regulates nasopharyngeal carcinoma radioresistance by targeting WNT2B in vitro.
abstract: Aberrant microRNA (miRNA) expression contributes to a series of malignant cancer behaviors, including radioresistance. Our previous study showed differential expression of miR-185-3p in post-radiation nasopharyngeal carcinoma (NPC) cells. To investigate the role of miR-185-3p in NPC radioresistance, CNE-2 and 5-8F cells were transfected with miR-185-3p mimic and miR-185-3p inhibitor, respectively. CCK-8 assay and colony formation experiment confirmed that the expression of miR-185-3p affected the radioresistance of NPC cells. A negative correlation between miR-185-3p and WNT2B expression was observed in NPC cells and tissues. Luciferase reporter assays confirmed that miR-185-3p directly targeted the coding region of WNT2B. Furthermore, we found radioresistance decreased in WNT2B-silenced NPC cells. Activation of the WNT2B/β-catenin pathway was accompanied by epithelial-mesenchymal transition biomarker changes in NPC. We concluded that miR-185-3p contributed to the radioresistance of NPC via modulation of WNT2B expression in vitro.
authors.affiliation: Department of Otolaryngology Head and Neck Surgery, Xiangya Hospital, Central South University, Changsha, China; Otolaryngology Major Disease Research Key Laboratory of Hunan Province, Changsha, Hunan, China.
authors.forename: Guo
authors.initials: G
authors.lastname: Li
authors.forename: Yunyun
authors.initials: Y
authors.lastname: Wang
authors.forename: Yong
authors.initials: Y
authors.lastname: Liu
authors.forename: Zhongwu
authors.initials: Z
authors.lastname: Su
authors.forename: Chao
authors.initials: C
authors.lastname: Liu
authors.forename: Shuling
authors.initials: S
authors.lastname: Ren
authors.forename: Tengbo
authors.initials: T
authors.lastname: Deng
authors.forename: Donghai
authors.initials: D
authors.lastname: Huang
authors.forename: Yongquan
authors.initials: Y
authors.lastname: Tian
authors.forename: Yuanzheng
authors.initials: Y
authors.lastname: Qiu
mesh_terms: D002277:Carcinoma; D045744:Cell Line, Tumor; D058750:Epithelial-Mesenchymal Transition; D015972:Gene Expression Regulation, Neoplastic / Q000528:radiation effects; D006023:Glycoproteins / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D066298:In Vitro Techniques; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D000077274:Nasopharyngeal Carcinoma; D009303:Nasopharyngeal Neoplasms / Q000378:metabolism* / Q000532:radiotherapy; D011836:Radiation Tolerance; D051153:Wnt Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D006023:Glycoproteins; C552431:MIRN185 microRNA, human; D035683:MicroRNAs; C115496:WNT2B protein, human; D051153:Wnt Proteins
keywords: Epithelial mesenchymal transition; WNT2B; miR-185-3p; nasopharyngeal carcinoma; radioresistance