title: Upregulation of miR-184 enhances the malignant biological behavior of human glioma cell line A172 by targeting FIH-1.
abstract: BACKGROUND
  In recent years, miRNAs have been suggested to play key roles in the formation and development of human glioma. The aim of this study is to investigate the effect and mechanism of miR-184 expression on the malignant behavior of human glioma cells.
  
  
  METHODS
  The relative quantity of miR-184 was determined in human glioma cell lines, and the expression of hypoxia-inducible factor-1 alpha (HIF-1α) was explored using western blotting. The effects of miR-184 inhibition on cell viability and apoptosis were explored, and the miR-184 target gene was determined using a luciferase assay and western blotting. Flow cytometry and Hoechst staining were used to evaluate cell growth and apoptosis. Matrigel invasion and scratch assays were performed to measure the ability of cell invasion and migration.
  
  
  RESULTS
  miR-184 and HIF-1α protein levels were significantly upregulated in human glioma cells. Downregulation of miR-184 inhibited cell viability and increased the HEB cell apoptotic rate. Luciferase and western blot assays verified that FIH-1 was the target gene of miR-184 and negatively controlled the protein level of HIF-1α. Inhibition of HIF-1α by siRNA facilitated the apoptosis of HEB cells and suppressed A172 cell invasion and migration.
  
  
  CONCLUSIONS
  miR-184 upregulation enhanced the malignant phenotype of human glioma cancer cells by reducing FIH-1 protein expression.
authors.affiliation: Department of Neurosurgery, The Second Affiliated Hospital of Harbin Medical University, Harbin, PR China.
authors.forename: Qinghua
authors.initials: Q
authors.lastname: Yuan
authors.forename: Weida
authors.initials: W
authors.lastname: Gao
authors.forename: Bo
authors.initials: B
authors.lastname: Liu
authors.forename: Wei
authors.initials: W
authors.lastname: Ye
mesh_terms: D017209:Apoptosis / Q000235:genetics; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics*; D049109:Cell Proliferation / Q000235:genetics; D002470:Cell Survival / Q000235:genetics; D015536:Down-Regulation / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics; D005910:Glioma / Q000235:genetics*; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D006899:Mixed Function Oxygenases / Q000235:genetics*; D009361:Neoplasm Invasiveness / Q000235:genetics*; D012097:Repressor Proteins / Q000235:genetics*; D015854:Up-Regulation / Q000235:genetics*
chemical_list: C534424:MIRN184 microRNA, human; D035683:MicroRNAs; D012097:Repressor Proteins; D006899:Mixed Function Oxygenases; C439790:HIF1AN protein, human