title: Pepper, chili (Capsicum annuum).
abstract: Pepper is a recalcitrant plant for Agrobacterium-mediated genetic transformation. Several obstacles to genetic transformation remain such as extremely low transformation rates; the choice of correct genotype is critical; and there is a high frequency of false positives due to direct shoot formation. Here, we report a useful protocol with a suitable selection method. The most important aspect of the pepper transformation protocol is selecting shoots growing from the callus, which is referred to as callus-mediated shoot formation. This protocol is a reproducible and reliable system for pepper transformation.
authors.affiliation: Biotechnology Institute, 113-141, Yanghwaro, Ganam, Yeoju, Gyeonggi, 469-885, South Korea, jungmin@nongwoobio.co.kr.
authors.forename: Jung
authors.initials: J
authors.lastname: Min
authors.forename: Sun Hee
authors.initials: SH
authors.lastname: Shin
authors.forename: En Mi
authors.initials: EM
authors.lastname: Jeon
authors.forename: Jung Mi
authors.initials: JM
authors.lastname: Park
authors.forename: Ji Young
authors.initials: JY
authors.lastname: Hyun
authors.forename: Chee Hark
authors.initials: CH
authors.lastname: Harn
mesh_terms: D000064:Acclimatization; D000383:Agriculture / Q000379:methods; D016960:Agrobacterium tumefaciens / Q000235:genetics; D002212:Capsicum / Q000235:genetics* / Q000254:growth & development; D005821:Genetic Techniques; D018525:Germination; D018517:Plant Roots / Q000235:genetics / Q000254:growth & development; D030821:Plants, Genetically Modified; D016133:Polymerase Chain Reaction / Q000379:methods; D012639:Seeds / Q000235:genetics; D012641:Selection, Genetic; D014169:Transformation, Bacterial