title: A simple and sensitive HPLC method based on pre-column fluorescence labelling for multiple classes of plant growth regulator determination in food samples.
abstract: The determination of trace plant growth regulator (PGR) has received more and more attentions in the field of phytophysiology and food safety. But the simple and sensitive method for simultaneously analysing multiple classes of PGR remains poorly investigated. In this study, a new pre-column fluorescence labelling method using 2-(11H-benzo[a]carbazol-11-yl)-ethyl-4-methylbenzenesulfonate (BCETS) as the labelling reagent has been developed for simultaneous determination of seven PGRs (i.e., indole-3-acetic acid, 3-indolybutyric acid, 3-indolepropionic acid, jasmonic acid, gibberellin A3, 1-naphthylacetic acid and 2-naphthaleneacetic acid) by HPLC with fluorescent detection (FLD). The proposed method offered the LOD of 0.34-0.73 ng/mL for seven PGRs, which were significantly lower than the reported methods. The crude extract without complex pre-treatments and purification was directly labelled by BCETS and analysed by HPLC-FLD, which facilitates the high-throughput sample screening. This method was proven to be inexpensive, simple, selective, sensitive, accurate and reliable for trace PGR determination.
authors.affiliation: Key Laboratory of Life-Organic Analysis of Shandong Province, Qufu Normal University, Qufu, People's Republic of China; College of Food Science and Technology, Guangdong Ocean University, Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Key Laboratory of Advanced Processing of Aquatic Products of Guangdong Higher Education Institution, Zhanjiang, People's Republic of China. Electronic address: 61254368@163.com.
authors.forename: Guoliang
authors.initials: G
authors.lastname: Li
authors.affiliation: College of Food Science and Technology, Guangdong Ocean University, Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Key Laboratory of Advanced Processing of Aquatic Products of Guangdong Higher Education Institution, Zhanjiang, People's Republic of China.
authors.forename: Shucheng
authors.initials: S
authors.lastname: Liu
authors.affiliation: Key Laboratory of Life-Organic Analysis of Shandong Province, Qufu Normal University, Qufu, People's Republic of China.
authors.forename: Zhiwei
authors.initials: Z
authors.lastname: Sun
authors.affiliation: Key Laboratory of Life-Organic Analysis of Shandong Province, Qufu Normal University, Qufu, People's Republic of China.
authors.forename: Lian
authors.initials: L
authors.lastname: Xia
authors.affiliation: Key Laboratory of Life-Organic Analysis of Shandong Province, Qufu Normal University, Qufu, People's Republic of China.
authors.forename: Guang
authors.initials: G
authors.lastname: Chen
authors.affiliation: Key Laboratory of Life-Organic Analysis of Shandong Province, Qufu Normal University, Qufu, People's Republic of China; Key Laboratory of Tibetan Medicine Research, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, People's Republic of China. Electronic address: jmyou6304@163.com.
authors.forename: Jinmao
authors.initials: J
authors.lastname: You
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005453:Fluorescence; D005504:Food Analysis; D010937:Plant Growth Regulators / Q000032:analysis*; D015203:Reproducibility of Results
chemical_list: D010937:Plant Growth Regulators
keywords: Food samples; HPLC; Plant growth regulators; Pre-column fluorescence labelling