title: Genetic Diversity of a Natural Population of Apple stem pitting virus Isolated from Apple in Korea.
abstract: Apple stem pitting virus (ASPV), of the Foveavirus genus in the family Betaflexiviridae, is one of the most common viruses of apple and pear trees. To examine variability of the coat protein (CP) gene from ASPV, eight isolates originating from 251 apple trees, which were collected from 22 apple orchards located in intensive apple growing areas of the North Gyeongsang and North Jeolla Provinces in Korea, were sequenced and compared. The nucleotide sequence identity of the CP gene of eight ASPV isolates ranged from 77.0 to 97.0%, while the amino acid sequence identity ranged from 87.7 to 98.5%. The N-terminal region of the viral CP gene was highly variable, whereas the C-terminal region was conserved. Genetic algorithm recombination detection (GARD) and single breakpoint recombination (SBP) analyses identified base substitutions between eight ASPV isolates at positions 54 and 57 and position 771, respectively. GABranch analysis was used to determine whether the eight isolates evolved due to positive selection. All values in the GABranch analysis showed a ratio of substitution rates at non-synonymous and synonymous sites (dNS/dS) below 1, suggestive of strong negative selection forces during ASPV CP history. Although negative selection dominated CP evolution in the eight ASPV isolates, SLAC and FEL tests identified four possible positive selection sites at codons 10, 22, 102, and 158. This is the first study of the ASPV genome in Korea.
authors.affiliation: Department of Horticultural Sciences, Seoul Women's University, Seoul 139-774, Korea.
authors.forename: Ju Yeon
authors.initials: JY
authors.lastname: Yoon
authors.affiliation: National Institute of Horticultural & Herbal Science, RDA, Suwon 440-706, Korea.
authors.forename: Jae Ho
authors.initials: JH
authors.lastname: Joa
authors.affiliation: National Institute of Horticultural & Herbal Science, RDA, Suwon 440-706, Korea.
authors.forename: Kyung San
authors.initials: KS
authors.lastname: Choi
authors.affiliation: National Institute of Horticultural & Herbal Science, RDA, Suwon 440-706, Korea.
authors.forename: Ki Seck
authors.initials: KS
authors.lastname: Do
authors.affiliation: National Institute of Horticultural & Herbal Science, RDA, Suwon 440-706, Korea.
authors.forename: Han Cheol
authors.initials: HC
authors.lastname: Lim
authors.affiliation: National Institute of Horticultural & Herbal Science, RDA, Suwon 440-706, Korea.
authors.forename: Bong Nam
authors.initials: BN
authors.lastname: Chung
keywords: CP; apple; apple stem pitting virus; genetic diversity