title: In vitro biological activity of aromadendrin-4'-methyl ether isolated from root extract of Ventilago madraspatana Gaertn with relevance to anticandidal activity.
abstract: In this study, antimicrobial and antioxidant activities of the hexane extract of the root of Ventilago madraspatana were evaluated. Based on the significant bioactivity of crude hexane extract, an active compound was purified from the root extract. The active compound was further purified and identified as aromodendrin-4'-methyl ether by the (1)H NMR spectrum. The isolated compound significantly inhibited Staphylococcus epidermidis with the lowest MIC and MBC at 78 μg/mL (P < 0.05). The compound also exhibited significant anticandidal activity with MIC and MBC values of 312 and 625 μg/mL, respectively. The radical scavenging activity of aromodendrin-4'-methyl ether was evident by its lower IC50 values of 60 μg/mL for DPPH scavenging and 3.2 μg/mL for ABTS scavenging. The compound also exhibited ferrous ion chelation and H2O2 scavenging activities. The study is an attempt to increase the industrial utility of V. madrasapatana.
authors.affiliation: a Department of Studies in Microbiology , University of Mysore , Manasagangotri, Mysore 570 006 , India.
authors.forename: P S
authors.initials: PS
authors.lastname: Rajesh
authors.forename: Pradeepa V
authors.initials: PV
authors.lastname: Samaga
authors.forename: V Ravishankar
authors.initials: VR
authors.lastname: Rai
authors.forename: K M Lokanatha
authors.initials: KM
authors.lastname: Rai
mesh_terms: D000890:Anti-Infective Agents / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology*; D002175:Candida / Q000187:drug effects; D005419:Flavonoids / Q000494:pharmacology*; D020128:Inhibitory Concentration 50; D008826:Microbial Sensitivity Tests; D015394:Molecular Structure; D010936:Plant Extracts / Q000737:chemistry*; D018517:Plant Roots / Q000737:chemistry; D027823:Rhamnaceae / Q000737:chemistry*; D013212:Staphylococcus epidermidis / Q000187:drug effects
chemical_list: D000890:Anti-Infective Agents; D000975:Antioxidants; D005419:Flavonoids; D010936:Plant Extracts; C080220:aromadedrin
keywords: DPPH; Ventilago madraspatana; antibacterial; anticandidal; antioxidant