title: DISP3 promotes proliferation and delays differentiation of neural progenitor cells.
abstract: DISP3 (PTCHD2), a sterol-sensing domain-containing protein, is highly expressed in neural tissue but its role in neural differentiation is unknown. In the present study we used a multipotent cerebellar progenitor cell line, C17.2, to investigate the impact of DISP3 on the proliferation and differentiation of neural precursors. We found that ectopically expressed DISP3 promotes cell proliferation and alters expression of genes that are involved in tumorigenesis. Finally, the differentiation profile of DISP3-expressing cells was altered, as evidenced by delayed expression of neural specific markers and a reduced capacity to undergo neural differentiation.
authors.affiliation: Institute of Molecular Genetics AS CR v.v.i., Vídeňská 1083, 142 20 Prague 4, Czech Republic.
authors.forename: Martina
authors.initials: M
authors.lastname: Zíková
authors.affiliation: Institute of Molecular Genetics AS CR v.v.i., Vídeňská 1083, 142 20 Prague 4, Czech Republic.
authors.forename: Jana
authors.initials: J
authors.lastname: Konířová
authors.affiliation: Institute of Molecular Genetics AS CR v.v.i., Vídeňská 1083, 142 20 Prague 4, Czech Republic.
authors.forename: Karolína
authors.initials: K
authors.lastname: Ditrychová
authors.affiliation: Institute of Molecular Genetics AS CR v.v.i., Vídeňská 1083, 142 20 Prague 4, Czech Republic.
authors.forename: Alicia
authors.initials: A
authors.lastname: Corlett
authors.affiliation: Institute of Molecular Genetics AS CR v.v.i., Vídeňská 1083, 142 20 Prague 4, Czech Republic.
authors.forename: Michal
authors.initials: M
authors.lastname: Kolář
authors.affiliation: Institute of Molecular Genetics AS CR v.v.i., Vídeňská 1083, 142 20 Prague 4, Czech Republic. Electronic address: bartunek@img.cas.cz.
authors.forename: Petr
authors.initials: P
authors.lastname: Bartůněk
mesh_terms: D001921:Brain / Q000166:cytology; D002454:Cell Differentiation; D002460:Cell Line; D049109:Cell Proliferation; D005786:Gene Expression Regulation; D006801:Humans; D050356:Lipid Metabolism; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D058953:Neural Stem Cells / Q000166:cytology* / Q000378:metabolism
chemical_list: C502279:DISP3 protein, human; D008565:Membrane Proteins
keywords: Cancer; Differentiation; Lipids; Neural cells; Proliferation