title: Rapid determination of IL-6 specific activity by flow cytometry.
abstract: Current methods to measure the specific activity of cytokines are based on the time-consuming determination of the growth curve of a sensitive cell line. Here, we present a faster alternative based on flow cytometry, by determining the dose-response curve of cellular response to a cytokine. By using World Health Organization (WHO) cytokine standards, rapid determination of cytokine specific activity is now possible, as it takes only a few hours to achieve, in comparison to days with the classical method thus allowing laboratories to rapidly and easily assess the potency of their cytokines.
authors.affiliation: Cellular Production, Research and Development, Héma-Québec, Canada.
authors.forename: Carl
authors.initials: C
authors.lastname: Simard
authors.affiliation: Cellular Production, Research and Development, Héma-Québec, Canada.
authors.forename: Marc
authors.initials: M
authors.lastname: Cloutier
authors.affiliation: Cellular Production, Research and Development, Héma-Québec, Canada.
authors.forename: Sonia
authors.initials: S
authors.lastname: Néron
mesh_terms: D000818:Animals; D002232:Carbocyanines; D045744:Cell Line, Tumor; D004306:Dose-Response Relationship, Immunologic; D005434:Flow Cytometry / Q000379:methods* / Q000592:standards; D005456:Fluorescent Dyes; D015870:Gene Expression; D015850:Interleukin-6 / Q000032:analysis* / Q000494:pharmacology; D051379:Mice; D022423:Myeloid Cells / Q000166:cytology / Q000187:drug effects* / Q000276:immunology; D050796:STAT3 Transcription Factor / Q000235:genetics / Q000276:immunology
chemical_list: C569686:Alexa Fluor 647; D002232:Carbocyanines; D005456:Fluorescent Dyes; D015850:Interleukin-6; D050796:STAT3 Transcription Factor; C494087:Stat3 protein, mouse
keywords: Bioassay; Flow cytometry; Fluorescent cell barcoding; IL-6; Specific activity; Stat-3