title: Dynamic 3D cell culture via a chemoselective photoactuated ligand.
abstract: A new strategy to create a dynamic scaffold for three-dimensional (3D) cell experiments based on a photo-activated cell adhesive peptide ligand is described. After polymerization, the inert matrix becomes cell adhesive by chemoselective modification through the conjugation of oxyamine-terminated ligands. Furthermore, spatial and temporal control of cell culture within the 3D matrix was achieved by the use of a biospecific photoprotected peptide and visualized by confocal microscopy.
authors.affiliation: Department of Chemistry, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599.
authors.forename: Nathan P
authors.initials: NP
authors.lastname: Westcott
authors.affiliation: Department of Chemistry, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599 and Department of Chemistry and Centre for Research on Biomolecular Interactions, York University, Toronto, Ontario M3J 1P3, Canada.
authors.forename: Wei
authors.initials: W
authors.lastname: Luo
authors.affiliation: Department of Chemistry, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599.
authors.forename: Jeffrey
authors.initials: J
authors.lastname: Goldstein
authors.affiliation: Department of Chemistry, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599 and Department of Chemistry and Centre for Research on Biomolecular Interactions, York University, Toronto, Ontario M3J 1P3, Canada.
authors.forename: Muhammad N
authors.initials: MN
authors.lastname: Yousaf
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D002478:Cells, Cultured; D005347:Fibroblasts / Q000187:drug effects / Q000502:physiology*; D008027:Light; D051379:Mice; D009924:Organ Culture Techniques / Q000379:methods*; D010455:Peptides / Q000378:metabolism*; D054457:Tissue Scaffolds
chemical_list: D010455:Peptides