title: Overexpression of the ITGAV gene is associated with progression and spread of colorectal cancer.
abstract: OBJECTIVE
  The interaction of neoplastic cells with the extracellular matrix is a critical event for the initiation of cancer invasion and metastasis. We evaluated the relationship between the expression of SPARC, ITGAV, THBS1 and VCAM-1 genes of extracellular matrix in the progression and dissemination of colorectal cancer (CRC).
  
  
  METHODS
  Adult patients (N=114) underwent resection of CRC. Gene expression in CRC was determined by quantitative real-time polymerase chain reaction (PCR). Protein expression was analyzed by immunohistochemistry (IHC). Correlation with pathway-related molecules (p53, Bcl-2, Ki-67, EGFR and VEGF) was assessed.
  
  
  RESULTS
  Tumors with perineural invasion showed overexpression (p=0.028) of the ITGAV gene with regard to cancers without perineural invasion and validation of the result through IHC expression of the corresponding proteins, was significant for the expression of ITGAV protein (p=0.001).
  
  
  CONCLUSIONS
  The overexpression of ITGAV gene was associated with higher progression and spread of CRC via perineural invasion.
authors.affiliation: Department of Surgery, São Paulo Federal University, São Paulo, Brazil Department of Surgery, ABC Medical School, Santo André, São Paulo, Brazil jaqueswaisberg@uol.com.br.
authors.forename: Jaques
authors.initials: J
authors.lastname: Waisberg
authors.affiliation: Department of Oncology, Barretos Cancer Hospital, Barretos, São Paulo, Brazil.
authors.forename: Luciano
authors.initials: L
authors.lastname: De Souza Viana
authors.affiliation: Department of Oncology, Barretos Cancer Hospital, Barretos, São Paulo, Brazil.
authors.forename: Renato José
authors.initials: RJ
authors.lastname: Affonso Junior
authors.affiliation: Department of Pathology, Barretos Cancer Hospital, Barretos, São Paulo, Brazil.
authors.forename: Sandra Regina Morini
authors.initials: SR
authors.lastname: Silva
authors.affiliation: Department of Oncology, Barretos Cancer Hospital, Barretos, São Paulo, Brazil.
authors.forename: Marcos Vinicius Araujo
authors.initials: MV
authors.lastname: Denadai
authors.affiliation: Department of Surgery, ABC Medical School, Santo André, São Paulo, Brazil.
authors.forename: Fernando Beani
authors.initials: FB
authors.lastname: Margeotto
authors.affiliation: Genetics Laboratory (GRAACC), São Paulo Federal University, São Paulo, Brazil.
authors.forename: Carolina Salinas
authors.initials: CS
authors.lastname: De Souza
authors.affiliation: Department of Surgery, São Paulo Federal University, São Paulo, Brazil.
authors.forename: Delcio
authors.initials: D
authors.lastname: Matos
mesh_terms: D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology*; D018450:Disease Progression; D005260:Female; D015870:Gene Expression; D006801:Humans; D007150:Immunohistochemistry; D039564:Integrin alphaV / Q000235:genetics* / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D060787:Neoplasm Grading; D009362:Neoplasm Metastasis; D009367:Neoplasm Staging; D015676:Osteonectin / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D019010:Vascular Cell Adhesion Molecule-1 / Q000235:genetics / Q000378:metabolism
chemical_list: D014408:Biomarkers, Tumor; D039564:Integrin alphaV; D015676:Osteonectin; C577241:SPARC protein, human; D019010:Vascular Cell Adhesion Molecule-1
keywords: Biological markers; colorectal neoplasms; extracellular matrix; gene expression; tumor markers