title: Serum vaspin levels and vaspin mRNA expression in subcutaneous adipose tissue in women with gestational diabetes mellitus.
abstract: OBJECTIVE
  To compare serum vaspin level and mRNA and protein levels of vaspin in adipose tissue in women with gestational diabetes mellitus (GDM) and normal glucose tolerance (NGR), along with the correlation between serum vaspin level with fasting insulin (FINS), homeostasis model assessment of insulin resistance (HOMA-IR) and birth-weight.
  
  
  METHODS
  Thirty-seven women with GDM and 36 with NGR were enrolled. Total cholesterol (TC), triglyceride (TG), high density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C), fasting plasma glucose (FPG), FINS and vaspin levels were measured. The mRNA and protein levels were detected using RT-PCR and Western blot. Pearson correlation analysis (PCA) was performed to reveal the correlation between serum vaspin level and FINS, HOMA-IR. Spearman correlation analysis (SCA) was conducted to examine the association between serum vaspin level and birth-weight.
  
  
  RESULTS
  HDL-C level in GDM was lower than NGR group (P<0.05), and there were no statistical differences in TC, TG, LDL-C, FPG, FINS and HOMA-IR between the two groups. Serum vaspin level, mRNA and protein expression levels of vaspin in GDM were higher than NGR group (P<0.05). Serum vaspin level was not significantly correlated with FINS and HOMA-IR, but had a positive correlation with birth-weight (P=0.023).
  
  
  CONCLUSIONS
  Serum vaspin level cannot serve as an independent predictor of IR. The increased serum vaspin level and increased vaspin mRNA and protein expression in adipose tissues in GDM women indicate that vaspin may be involved in the pathogenesis of GDM, but its exact mechanism needs further study.
authors.affiliation: Department of Gynecology and Obstetrics, Shanghai Jiao Tong University, International Peace Maternity and Child Health Hospital, 910 Hengshan Road, Shanghai 200030, China.
authors.forename: Wei Qian
authors.initials: WQ
authors.lastname: Mm
authors.affiliation: Department of Gynecology and Obstetrics, Shanghai Jiao Tong University, International Peace Maternity and Child Health Hospital, 910 Hengshan Road, Shanghai 200030, China. Electronic address: fjianxia@163.com.
authors.forename: Jianxia
authors.initials: J
authors.lastname: Fan
authors.affiliation: Department of Gynecology and Obstetrics, Shanghai Jiao Tong University, International Peace Maternity and Child Health Hospital, 910 Hengshan Road, Shanghai 200030, China.
authors.forename: Shuzin
authors.initials: S
authors.lastname: Khor
authors.affiliation: Department of Gynecology and Obstetrics, Shanghai Jiao Tong University, International Peace Maternity and Child Health Hospital, 910 Hengshan Road, Shanghai 200030, China.
authors.forename: Mengfan
authors.initials: M
authors.lastname: Song
authors.affiliation: Department of Gynecology and Obstetrics, Shanghai Jiao Tong University, International Peace Maternity and Child Health Hospital, 910 Hengshan Road, Shanghai 200030, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Hong
authors.affiliation: Department of Gynecology and Obstetrics, Shanghai Jiao Tong University, International Peace Maternity and Child Health Hospital, 910 Hengshan Road, Shanghai 200030, China.
authors.forename: Xiaobei
authors.initials: X
authors.lastname: Dai
mesh_terms: D000328:Adult; D000596:Amino Acids; D015415:Biomarkers / Q000097:blood; D001724:Birth Weight; D001786:Blood Glucose / Q000378:metabolism; D015992:Body Mass Index; D016022:Case-Control Studies; D008076:Cholesterol, HDL / Q000097:blood; D008078:Cholesterol, LDL / Q000097:blood; D002857:Chromium; D016640:Diabetes, Gestational / Q000097:blood* / Q000235:genetics / Q000378:metabolism; D005260:Female; D006706:Homeostasis; D006801:Humans; D007328:Insulin / Q000097:blood; D007333:Insulin Resistance; D009539:Nicotinic Acids; D011247:Pregnancy; D011446:Prospective Studies; D012333:RNA, Messenger / Q000378:metabolism*; D015843:Serpins / Q000097:blood* / Q000235:genetics / Q000378:metabolism; D050151:Subcutaneous Fat / Q000378:metabolism*; D014280:Triglycerides / Q000097:blood
chemical_list: D000596:Amino Acids; D015415:Biomarkers; D001786:Blood Glucose; D008076:Cholesterol, HDL; D008078:Cholesterol, LDL; D007328:Insulin; D009539:Nicotinic Acids; D012333:RNA, Messenger; C507339:SERPINA12 protein, human; D015843:Serpins; D014280:Triglycerides; C015240:glucose tolerance factor; D002857:Chromium
keywords: Gestational diabetes mellitus; Serum vaspin; Subcutaneous adipose tissue; Vaspin mRNA