title: Molecular Characterization and Variation of the Broad bean wilt virus 2 Isolates Based on Analyses of Complete Genome Sequences.
abstract: The full-genome sequences of fourteen isolates of Broad bean wilt virus 2 (BBWV2), collected from broad bean, pea, spinach, bell pepper and paprika plants in Korea during the years 2006-2012, were determined and analyzed comparatively along with fifteen previously reported BBWV2 genome sequences. Sequence analyses showed that RNA-1 and RNA-2 sequences of BBWV2 Korean isolates consisted of 5950-5956 and 3568-3604 nucleotides, respectively. Full-length genome sequence-based phylogenetic analyses revealed that the BBWV2 Korean isolates could be divided into three major groups comprising GS-I (isolates BB2 and RP7) along with isolate IP, GS-II (isolates BB5, P2, P3 and RP3) along with isolate B935, and GS-III including 16 BBWV2 Korean isolates. Interestingly, GS-III appears to be newly emerged and predominant in Korea. Recombination analyses identified two recombination events in the analyzed BBWV2 population: one in the RNA-1 of isolate K and another one in the RNA-2 of isolate XJ14-3. However, no recombination events were detected in the other 21 Korean isolates. On the other hand, out of 29 BBWV2 isolates, 16 isolates were found to be reassortants, of which each RNA segment (i.e. RNA1 and RNA2) was originated from different parental isolates. Our findings suggested that reassortment rather than recombination is a major evolutionary force in the genetic diversification of BBWV population in Korea.
authors.affiliation: Crop Protection Division, National Academy of Agricultural Science, Suwon 441-707, Korea ; Department of Plant Medicine, Chungbuk National University, Cheongju 361-763, Korea.
authors.forename: Hae-Ryun
authors.initials: HR
authors.lastname: Kwak
authors.affiliation: Crop Protection Division, National Academy of Agricultural Science, Suwon 441-707, Korea.
authors.forename: Mi-Kyeong
authors.initials: MK
authors.lastname: Kim
authors.affiliation: Crop Protection Division, National Academy of Agricultural Science, Suwon 441-707, Korea.
authors.forename: Ye-Ji
authors.initials: YJ
authors.lastname: Lee
authors.affiliation: Crop Protection Division, National Academy of Agricultural Science, Suwon 441-707, Korea.
authors.forename: Jang-Kyun
authors.initials: JK
authors.lastname: Seo
authors.affiliation: Crop Protection Division, National Academy of Agricultural Science, Suwon 441-707, Korea.
authors.forename: Jeong-Soo
authors.initials: JS
authors.lastname: Kim
authors.affiliation: Department of Agricultural Biotechnology and Plant Genomics and Breeding Institute, Seoul National University, Seoul 151-921, Korea.
authors.forename: Kook-Hyung
authors.initials: KH
authors.lastname: Kim
authors.affiliation: Department of Plant Medicine, Chungbuk National University, Cheongju 361-763, Korea.
authors.forename: Byeongjin
authors.initials: B
authors.lastname: Cha
authors.affiliation: Crop Protection Division, National Academy of Agricultural Science, Suwon 441-707, Korea.
authors.forename: Hong-Soo
authors.initials: HS
authors.lastname: Choi
keywords: BBWV2; phylogenetic analyses; reassortment; recombination