title: Sulphation pattern analysis of chemically sulphated polysaccharide LbGp1 from Lycium barbarum by GC-MS.
abstract: The polysaccharide LbGp1 from Lycium barbarum L. was sulphated with sulphur trioxide-pyridine complex in DMF, yielding two sulphated polysaccharides, which were LbGp1-OL-SL with 13.7% sulphate content, and LbGp1-OL-SH with 27.4% sulphate content. The sulphation patterns were analysed using a GC-MS strategy. After a series of sequential chemical derivatisations, the sulphated polysaccharides were converted to partially methylated alditol acetates. All the sulphate groups were replaced by acetyl groups, maintaining the positional information of the original sulphation pattern. The number and position of sulphate substitutions were deduced by comparing the relative molar ratio from methylation analysis between native polysaccharide and sulphated derivatives. In LbGp1-OL-SL, 12.65% of sulphation located on C-5 of Ara, only 0.69% and 0.34% of sulphation occurred on C-4 and C-6 Gal, respectively; while in LbGp1-OL-SH, 24.96% of sulphate groups were found at C-5 of Ara, and 0.40% and 2.02% of sulphate groups were found at C-4 and C-6 Gal, respectively.
authors.affiliation: Educational Ministry Key Laboratory of Resource Biology and Biotechnology in Western China, Life Science College, Northwest University, Xi'an 710069, PR China.
authors.forename: Yujiao
authors.initials: Y
authors.lastname: Sun
authors.affiliation: Educational Ministry Key Laboratory of Resource Biology and Biotechnology in Western China, Life Science College, Northwest University, Xi'an 710069, PR China.
authors.forename: Wei
authors.initials: W
authors.lastname: Sun
authors.affiliation: Educational Ministry Key Laboratory of Resource Biology and Biotechnology in Western China, Life Science College, Northwest University, Xi'an 710069, PR China.
authors.forename: Jiatong
authors.initials: J
authors.lastname: Guo
authors.affiliation: Educational Ministry Key Laboratory of Resource Biology and Biotechnology in Western China, Life Science College, Northwest University, Xi'an 710069, PR China.
authors.forename: Xiuting
authors.initials: X
authors.lastname: Hu
authors.affiliation: Educational Ministry Key Laboratory of Resource Biology and Biotechnology in Western China, Life Science College, Northwest University, Xi'an 710069, PR China.
authors.forename: Guiping
authors.initials: G
authors.lastname: Gong
authors.affiliation: Educational Ministry Key Laboratory of Resource Biology and Biotechnology in Western China, Life Science College, Northwest University, Xi'an 710069, PR China.
authors.forename: Linjuan
authors.initials: L
authors.lastname: Huang
authors.affiliation: National Glycoengineering Research Center, Shandong University, Jinan 250012, PR China.
authors.forename: Hongzhi
authors.initials: H
authors.lastname: Cao
authors.affiliation: Educational Ministry Key Laboratory of Resource Biology and Biotechnology in Western China, Life Science College, Northwest University, Xi'an 710069, PR China. Electronic address: wangzhf@nwu.edu.cn.
authors.forename: Zhongfu
authors.initials: Z
authors.lastname: Wang
mesh_terms: D032305:Lycium / Q000737:chemistry*; D013058:Mass Spectrometry / Q000379:methods*; D011134:Polysaccharides / Q000737:chemistry*; D013431:Sulfates / Q000737:chemistry*
chemical_list: D011134:Polysaccharides; D013431:Sulfates
keywords: GC–MS; Lycium barbarum L.; Sulphate modification; Sulphation pattern determination