title: Construction of infectious cDNA clones derived from the potyviruses clover yellow vein virus and bean yellow mosaic virus.
abstract: Infectious cDNA clones are now indispensible tools for the genetic analysis of viral factors involved in viral virulence and host resistance. In addition, infectious cDNA-derived virus vectors that express foreign genes in infected plants enable the production of useful proteins at low cost and can confer novel crop traits. We constructed infectious cDNA clones derived from two potyviruses, Clover yellow vein virus and Bean yellow mosaic virus, which infect legume plants and cause disease. Here, we present our procedure for constructing these potyvirus infectious clones.
authors.affiliation: Pathogen-Plant Interactions Group, Plant Breeding Science, Research Faculty of Agriculture, Hokkaido University, Kita-ku, Kita 9, Nishi 9, Sapporo, 060-8589, Japan, knakahar@res.agr.hokudai.ac.jp.
authors.forename: Kenji S
authors.initials: KS
authors.lastname: Nakahara
authors.forename: Kei
authors.initials: K
authors.lastname: Nishino
authors.forename: Ichiro
authors.initials: I
authors.lastname: Uyeda
mesh_terms: D001483:Base Sequence; D003001:Cloning, Molecular / Q000379:methods; D018076:DNA, Complementary; D008967:Molecular Biology / Q000379:methods*; D008969:Molecular Sequence Data; D017800:Potyvirus / Q000235:genetics* / Q000472:pathogenicity*
chemical_list: D018076:DNA, Complementary