title: Expression of interleukins 7 & 8 in peripheral blood mononuclear cells from patients with metabolic syndrome: a preliminary study.
abstract: OBJECTIVE
  Metabolic syndrome (MS) is a common but serious public health problem in developed countries. Chronic inflammation plays a key role in MS. Interleukins (IL)-7 and 8 are considered to have proinflammatory effects and may be involved in the pathogenesis of MS. Therefore, the aim of this study was to determine gene expression level of IL-7 and IL-8 in peripheral blood mononuclear cells (PBMCs) of patients with MS compared to healthy control subjects.
  
  
  METHODS
  Using real-time RT-PCR, the relative amounts of IL-7 and IL-8 mRNA were determined in PBMCs from 20 female patients with MS and compared with those of 20 healthy control subjects. Biochemical and anthropometric parameters of MS were also assessed.
  
  
  RESULTS
  Total cholesterol, triglyceride, and fasting blood sugar were significantly higher in MS patients compared to healthy subjects. There were no significant differences in HDLc and LDLc between the two groups. IL-8 expression in PBMC was significantly decreased in MS versus control subjects (fold of change was 0.395±0.1824), while no difference in the IL-7 expression was detected between them. IL-8 expression had negative correlation with MS components especially with triglyceride and total cholesterol (r=0.5, P<0.001).
  
  
  CONCLUSIONS
  In this preliminary study, no detectable differences were found in IL-7 expression and decreased expression of IL-8 in PBMCs of MS patients as compared to those of control subjects. Study on a larger population and investigating the mechanisms involved can reveal more details.
authors.forename: M
authors.initials: M
authors.lastname: Saidijam
authors.forename: A S
authors.initials: AS
authors.lastname: Tootoonchi
authors.affiliation: Research Center for Molecular Medicine, Hamadan University of Medical Sciences, Hamadan, India.
authors.forename: M T
authors.initials: MT
authors.lastname: Goodarzi
authors.forename: T
authors.initials: T
authors.lastname: Hassanzadeh
authors.forename: S H
authors.initials: SH
authors.lastname: Borzuei
authors.forename: R
authors.initials: R
authors.lastname: Yadegarazari
authors.forename: N
authors.initials: N
authors.lastname: Shabab
mesh_terms: D001786:Blood Glucose; D016022:Case-Control Studies; D002784:Cholesterol / Q000097:blood; D017931:DNA Primers / Q000235:genetics; D005260:Female; D005786:Gene Expression Regulation / Q000502:physiology*; D006801:Humans; D015851:Interleukin-7 / Q000378:metabolism*; D016209:Interleukin-8 / Q000378:metabolism*; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D024821:Metabolic Syndrome / Q000378:metabolism*; D060888:Real-Time Polymerase Chain Reaction; D018709:Statistics, Nonparametric; D014280:Triglycerides / Q000097:blood
chemical_list: D001786:Blood Glucose; D017931:DNA Primers; D015851:Interleukin-7; D016209:Interleukin-8; D014280:Triglycerides; D002784:Cholesterol