title: Intracellular Signaling Mechanisms Pharmacological Action of Jasminum amplexicaule Buch.-Ham. (Oleaceae) on Gastrointestinal Secretion.
abstract: Jasminum amplexicaule Buch-Ham. (Oleaceae) has been commonly used in the traditional medicine in dysentery, diarrhoea and bellyache in China. In the present work, the methanol extract of Jasminum amplexicaule (JME) was examined for pharmacology on human colonic epithelial cell line T84 by the short-circuit current technique. The results showed that pretreatment of T84 cells with JME produced a concentration-dependent (0-1000 μg/mL. EC50 = 0.055 mg/ mL) inhibition effect on adrenalin (Adr.)-induced Cl- secretion. The maximal response was observed at 200 μg/mL. It has been demonstrated that JME has a direct effect on the enterocyte. Our results also demonstrated that the JME exerted inhibitory effect on gastrointestinal Cl(-)secretion that effected by acting on basolateral β-adrenoreceptors. These results suggested that the Chinese traditional medicine of JME can be used for the treatment of acute diarrhea and bellyache.
authors.affiliation: The First Affiliated Hospital of Sun Yat-Sen University, Guangzhou 510080, China.
authors.forename: Zhenhua
authors.initials: Z
authors.lastname: Gao
authors.affiliation: The First Affiliated Hospital of Sun Yat-Sen University, Guangzhou 510080, China.
authors.forename: Junqiang
authors.initials: J
authors.lastname: Yin
authors.affiliation: The Insititute of Biology, Guizhou Academy of Sciences, Guiyang 550009, China.
authors.forename: Xiaolin
authors.initials: X
authors.lastname: Xie
authors.affiliation: The Insititute of Biology, Guizhou Academy of Sciences, Guiyang 550009, China.
authors.forename: Hanwu
authors.initials: H
authors.lastname: Long
authors.affiliation: The Insititute of Biology, Guizhou Academy of Sciences, Guiyang 550009, China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Qi
authors.affiliation: The Insititute of Biology, Guizhou Academy of Sciences, Guiyang 550009, China.
authors.forename: Changhu
authors.initials: C
authors.lastname: Lin
authors.affiliation: The First Affiliated Hospital of Sun Yat-Sen University, Guangzhou 510080, China.
authors.forename: Liangcai
authors.initials: L
authors.lastname: Wu
keywords: Anti-diarrhoea; Jasminum amplexicaule; Secretion; T84 cell