title: Hypertension and vulnerability to hemorrhagic shock in a rat model.
abstract: Trauma mortality may be increased in the presence of preexisting diseases such as chronic hypertension. We hypothesized that systemic and microvascular alterations accompanying chronic hypertension would increase the vulnerability to hemorrhage relative to normotensive controls in a rat model of hemorrhagic shock. We present a novel comparative hemorrhage model of shock vulnerability, quantified by "vulnerability curves" expressing physiological response to hemorrhage as a function of three matched shock metrics: cumulative blood volume, mean arterial pressure (MAP), and oxygen delivery (Do2). Responses were central hemodynamics and respiratory and muscle oxygenation obtained for one hypertensive (spontaneously hypertensive [SHR]) and two normotensive (Sprague-Dawley, Wistar-Kyoto) rat strains. Hemorrhagic shock was induced by incremental (0.5 mL) hemorrhage to cardiovascular collapse in anesthetized and mechanically ventilated animals. Shock vulnerability of SHR rats was primarily pressure-driven; in general, SHR exhibited the expected patterns of more rapid deterioration in MAP and Vo2 over smaller ranges of blood loss and Do2. Sternotomy-related depression of CO and thus Do2 in SHR meant that we could not test hypotheses related to the role of Do2 and contribution to perfusion differences between normotensive and hypertensive subjects. Insensitivity of lactate to strain effects suggests that lactate may be a reliable biomarker of shock status. Unexpected similarities between Wistar-Kyoto and SHR suggest strain-related effects other than those related to hypertension per se contribute to hemorrhage response; body size effects and genetic relationships could not be ruled out. Future studies should incorporate phylogenetically based methods to examine the role of hypertension and physiological response to hemorrhage across multiple strains.
authors.affiliation: Departments of *Anesthesiology, †Emergency Medicine, and ‡Physiology, Virginia Commonwealth University Medical Center, Richmond, Virginia.
authors.forename: Penny S
authors.initials: PS
authors.lastname: Reynolds
authors.forename: Kyle Seokhan
authors.initials: KS
authors.lastname: Song
authors.forename: Francisco J
authors.initials: FJ
authors.lastname: Tamariz
authors.forename: R
authors.initials: R
authors.lastname: Wayne Barbee
mesh_terms: D000818:Animals; D001794:Blood Pressure / Q000502:physiology; D006439:Hemodynamics / Q000502:physiology; D006973:Hypertension / Q000150:complications* / Q000378:metabolism / Q000503:physiopathology; D019344:Lactic Acid / Q000097:blood; D008297:Male; D018482:Muscle, Skeletal / Q000378:metabolism; D010101:Oxygen Consumption / Q000502:physiology; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY; D017207:Rats, Sprague-Dawley; D012771:Shock, Hemorrhagic / Q000209:etiology* / Q000378:metabolism / Q000503:physiopathology
chemical_list: D019344:Lactic Acid