title: Oat (Avena sativa L.).
abstract: Agrobacterium-mediated transformation is a suitable method to transform different cultivars using different systems of A. tumefaciens strains and binary vectors as well as selection cassettes. We describe here a detailed protocol for two cultivars, one naked and one husked, using the AGL1 strain and the pGreen vector containing the nptII selection cassette ( http://www.pgreen.ac.uk/ ), suitable for oat as well as other cereals. The pGreen vector system was recently developed for pBract ( http://www.bract.org/ ) and its transformation ability for cereals was proved. Assuming our experience and the latest knowledge on Agrobacterium-mediated transformation of cereals, we suggest using in the protocol one of the newly developed pBract or pCAMBIA ( http://www.cambia.org/daisy/cambia/ ) vector systems which carry different selection cassettes. The commonly used selection genes nptII, bar, and hpt were proved to be applicable for oat transformation and might be used as needed.
authors.affiliation: Department of Functional Genomics, Plant Breeding and Acclimatization Institute, National Research Institute, Radzikow, 05-870, Błonie, Poland.
authors.forename: Sebastian
authors.initials: S
authors.lastname: Gasparis
authors.forename: Anna
authors.initials: A
authors.lastname: Nadolska-Orczyk
mesh_terms: D000064:Acclimatization; D016960:Agrobacterium tumefaciens / Q000235:genetics; D018554:Avena / Q000235:genetics* / Q000254:growth & development; D018920:Coculture Techniques; D005821:Genetic Techniques; D005822:Genetic Vectors; D030821:Plants, Genetically Modified; D012639:Seeds / Q000235:genetics / Q000254:growth & development; D012641:Selection, Genetic; D014170:Transformation, Genetic