title: Molecular cloning, characterization and expression analysis of the SAMS gene during adventitious root development in IBA-induced tetraploid black locust.
abstract: S-Adenosylmethionine synthetase (SAMS) catalyzes the synthesis of S-adenosylmethionine (SAM), a precursor for ethylene and polyamine biosynthesis. Here, we report the isolation of the 1498 bp full-length cDNA sequence encoding tetraploid black locust (Robinia pseudoacacia L.) SAMS (TrbSAMS), which contains an open reading frame of 1179 bp encoding 392 amino acids. The amino acid sequence of TrbSAMS has more than 94% sequence identity to SAMSs from other plants, with a closer phylogenetic relationship to SAMSs from legumes than to SAMS from other plants. The TrbSAMS monomer consists of N-terminal, central, and C-terminal domains. Subcellular localization analysis revealed that the TrbSAMS protein localizes mainly to in the cell membrane and cytoplasm of onion epidermal cells and Arabidopsis mesophyll cell protoplasts. Indole-3-butyric acid (IBA)-treated cuttings showed higher levels of TrbSAMS transcript than untreated control cuttings during root primordium and adventitious root formation. TrbSAMS and its downstream genes showed differential expression in shoots, leaves, bark, and roots, with the highest expression observed in bark. IBA-treated cuttings also showed higher SAMS activity than control cuttings during root primordium and adventitious root formation. These results indicate that TrbSAMS might play an important role in the regulation of IBA-induced adventitious root development in tetraploid black locust cuttings.
authors.affiliation: State Key Laboratory of Soil Erosion and Dryland Farming on the Loess Plateau, Northwest A&F University, Yangling, China.
authors.forename: Jine
authors.initials: J
authors.lastname: Quan
authors.affiliation: State Key Laboratory of Soil Erosion and Dryland Farming on the Loess Plateau, Northwest A&F University, Yangling, China.
authors.forename: Sheng
authors.initials: S
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Soil Erosion and Dryland Farming on the Loess Plateau, Northwest A&F University, Yangling, China.
authors.forename: Chunxia
authors.initials: C
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Soil Erosion and Dryland Farming on the Loess Plateau, Northwest A&F University, Yangling, China.
authors.forename: Sen
authors.initials: S
authors.lastname: Meng
authors.affiliation: State Key Laboratory of Soil Erosion and Dryland Farming on the Loess Plateau, Northwest A&F University, Yangling, China.
authors.forename: Zhong
authors.initials: Z
authors.lastname: Zhao
authors.affiliation: State Key Laboratory of Soil Erosion and Dryland Farming on the Loess Plateau, Northwest A&F University, Yangling, China.
authors.forename: Xuexuan
authors.initials: X
authors.lastname: Xu
mesh_terms: D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D005030:Ethylenes / Q000378:metabolism; D018506:Gene Expression Regulation, Plant / Q000187:drug effects*; D017343:Genes, Plant; D007211:Indoles / Q000494:pharmacology*; D008716:Methionine Adenosyltransferase / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010802:Phylogeny; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D018517:Plant Roots / Q000187:drug effects / Q000235:genetics / Q000254:growth & development*; D011073:Polyamines / Q000378:metabolism; D017433:Protein Structure, Secondary; D031301:Robinia / Q000033:anatomy & histology / Q000187:drug effects / Q000201:enzymology / Q000235:genetics*; D016415:Sequence Alignment; D020539:Sequence Analysis, Protein; D013347:Subcellular Fractions / Q000201:enzymology; D057891:Tetraploidy
chemical_list: D005030:Ethylenes; D007211:Indoles; D010940:Plant Proteins; D011073:Polyamines; C014612:indolebutyric acid; C036216:ethylene; D008716:Methionine Adenosyltransferase