title: [Proteomic methods of protein separation and analysis].
abstract: The review briefly presents principles and stages of execution of such highly effective methods of protein separation as capillary, 2D gel-electrophoresis, liquid chromatography, the possibility of their successful combination with tandem mass-spectrometry and application in various fields of proteomics. The main problems of proteomic analysis as well as ways of solving by using mass-spectrometry are examined. 2 main approaches during protein identification are described in the review, characteristics and possibilities of various top-down and bottom-up proteomic analytical programs are provided.
authors.forename: T A
authors.initials: TA
authors.lastname: Polunina
authors.forename: Iu S
authors.initials: IuS
authors.lastname: Varshavskaia
authors.forename: G V
authors.initials: GV
authors.lastname: Grigor'eva
authors.forename: Iu M
authors.initials: IuM
authors.lastname: Krasnov
mesh_terms: D002853:Chromatography, Liquid; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D011506:Proteins / Q000235:genetics / Q000302:isolation & purification*; D040901:Proteomics
chemical_list: D011506:Proteins