title: Immunomodulatory effects of pCramoll and rCramoll on peritoneal exudate cells (PECs) infected and non-infected with Staphylococcus aureus.
abstract: Peritoneal exudate cells (PECs) play important roles in host defense against Staphylococcus aureus and other pathogens. In this study we evaluated the potentials of native (pCramoll or Cramoll 1,4) and recombinant (rCramoll) lectins from seeds of Cratylia mollis as immunomodulatory tools on mice PECs infected and non-infected with S. aureus. Both lectins significantly enhanced nitric oxide, superoxide and cytokines (IL-1β, IL-6, IFN-γ and TNF-α). pCramoll and rCramoll downregulated the induction of TNF-α and IL-6 and upregulated the expression of IL-1β, IFN-γ in S. aureus infected PECs. Phagocytic activity of S. aureus was also enhanced in 27.1% and 22.47% by pCramoll and rCramoll, respectively. Our results showed that pCramoll induced stronger effects than rCramoll, which could be explained by the different hemagglutinating activities of C. mollis isolectins and nature fragmentation, although the biologic meaning should be studied in detail using in vivo models. Future works will be focused on the molecular mechanisms involved in these actions, using in vitro and in vivo models, to support the use of these lectins as biotechnological tool in immunological studies.
authors.forename: Luís Cláudio Nascimento
authors.initials: LC
authors.lastname: da Silva
authors.forename: Neyla Maria Pereira
authors.initials: NM
authors.lastname: Alves
authors.forename: Maria Carolina Accioly Brelaz
authors.initials: MC
authors.lastname: de Castro
authors.forename: Valéria Rêgo Alves
authors.initials: VR
authors.lastname: Pereira
authors.forename: Nathalia Varejão Nogueira
authors.initials: NV
authors.lastname: da Paz
authors.forename: Luana Cassandra Breitenbach Barroso
authors.initials: LC
authors.lastname: Coelho
authors.forename: Regina Célia Bressan Queiroz
authors.initials: RC
authors.lastname: de Figueiredo
authors.forename: Maria Tereza dos Santos
authors.initials: MT
authors.lastname: Correia
mesh_terms: D000818:Animals; D002470:Cell Survival / Q000187:drug effects / Q000276:immunology; D016207:Cytokines / Q000096:biosynthesis; D004195:Disease Models, Animal; D005122:Exudates and Transudates / Q000166:cytology*; D007155:Immunologic Factors / Q000494:pharmacology*; D037102:Lectins / Q000494:pharmacology*; D017737:Macrophages, Peritoneal / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism / Q000382:microbiology; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D051379:Mice; D009569:Nitric Oxide / Q000096:biosynthesis; D010587:Phagocytosis / Q000187:drug effects / Q000276:immunology; D013203:Staphylococcal Infections / Q000188:drug therapy / Q000276:immunology; D013211:Staphylococcus aureus / Q000276:immunology; D013481:Superoxides / Q000378:metabolism
chemical_list: D016207:Cytokines; D007155:Immunologic Factors; D037102:Lectins; D013481:Superoxides; D009569:Nitric Oxide