title: Diagnostic performance study of an antigen microarray for the detection of antiphospholipid antibodies in human serum.
abstract: BACKGROUND
  The parallelization of clinically relevant antigens in a microarray format is of growing importance due to the ability to measure multiple antigen-antibody interactions. With the development of a microarray for the detection of antiphospholipid antibodies we focussed on one important autoimmune disease that is still diagnostically challenging. Reasons are the heterogeneity of the autoantibodies and the unspecific clinical symptoms.
  
  
  METHODS
  For the covalent immobilization of antigenic structures, glass transducers were coated with 11-aminoundecyltrimethoxysilane (11-AUTMS). In total 35 antiphospholipid syndrome (APS) patients, six patients with lupus erythematosus and 24 healthy controls were investigated on a microarray format using polarized imaging reflectometric interference spectroscopy.
  
  
  RESULTS
  The novel surface modification based on the short derivative 11-AUTMS resulted in a selective biosensor allowing a clear differentiation of patient and control samples. It combined proteinogenic as well as phospholipid-derived antigens, namely β2-glycoprotein I (β2-GPI), prothrombin, cardiolipin (CL) and a β2-GPI/CL complex. With optimized regeneration conditions, up to 20 consecutive measurements could be performed on one chip. Sensitivity was determined to be 0.800-0.929, specificity was between 0.733 and 0.969, depending on the respective antigen.
  
  
  CONCLUSIONS
  Multiplexed determination of serological parameters has a great potential. We have shown that our biosensor is capable of detecting four different APS relevant antibodies in parallel exhibiting a sensitivity and specificity comparable to existing ELISA methods.
authors.forename: Aline R
authors.initials: AR
authors.lastname: Schindler
authors.forename: Oliver
authors.initials: O
authors.lastname: Bleher
authors.forename: Markus A
authors.initials: MA
authors.lastname: Thaler
authors.forename: Carmen J
authors.initials: CJ
authors.lastname: Kocot
authors.forename: Udo
authors.initials: U
authors.lastname: Steigerwald
authors.forename: Günther
authors.initials: G
authors.lastname: Proll
authors.forename: Günter
authors.initials: G
authors.lastname: Gauglitz
authors.forename: Peter B
authors.initials: PB
authors.lastname: Luppa
mesh_terms: D017152:Antibodies, Antiphospholipid / Q000032:analysis* / Q000276:immunology; D000941:Antigens / Q000737:chemistry / Q000276:immunology*; D016736:Antiphospholipid Syndrome / Q000175:diagnosis*; D005898:Glass / Q000737:chemistry; D006801:Humans; D055767:Immobilized Proteins / Q000737:chemistry / Q000276:immunology; D046228:Microarray Analysis / Q000379:methods*; D053482:beta 2-Glycoprotein I / Q000737:chemistry / Q000276:immunology
chemical_list: D017152:Antibodies, Antiphospholipid; D000941:Antigens; D055767:Immobilized Proteins; D053482:beta 2-Glycoprotein I