title: Molecularly imprinted solid-phase extraction of glutathione from urine samples.
abstract: Molecularly imprinted polymer (MIP) particles for glutathione were synthesized through iniferter-controlled living radical precipitation polymerization (IRPP) under ultraviolet radiation at ambient temperature. Static adsorption, solid-phase extraction, and high-performance liquid chromatography were carried out to evaluate the adsorption properties and selective recognition characteristics of the polymers for glutathione and its structural analogs. The obtained IRPP-MIP particles exhibited a regularly spherical shape, rapid binding kinetics, high imprinting factor, and high selectivity compared with the MIP particles prepared using traditional free-radical precipitation polymerization. The selective separation and enrichment of glutathione from the mixture of glycyl-glycine and glutathione disulfide could be achieved on the IRPP-MIP cartridge. The recoveries of glutathione, glycyl-glycine, and glutathione disulfide were 95.6% ± 3.65%, 29.5% ± 1.26%, and 49.9% ± 1.71%, respectively. The detection limit (S/N=3) of glutathione was 0.5 mg·L(-1). The relative standard deviations (RSDs) for 10 replicate detections of 50 mg·L(-1) of glutathione were 5.76%, and the linear range of the calibration curve was 0.5 mg·L(-1) to 200 mg·L(-1) under optimized conditions. The proposed approach was successfully applied to determine glutathione in spiked human urine samples with recoveries of 90.24% to 96.20% and RSDs of 0.48% to 5.67%.
authors.affiliation: School of Natural and Applied Science, Northwestern Polytechnical University, Xi'an 710072, PR China. Electronic address: songrenyuan0726@163.com.
authors.forename: Renyuan
authors.initials: R
authors.lastname: Song
authors.affiliation: School of Natural and Applied Science, Northwestern Polytechnical University, Xi'an 710072, PR China.
authors.forename: Xiaoling
authors.initials: X
authors.lastname: Hu
authors.affiliation: School of Natural and Applied Science, Northwestern Polytechnical University, Xi'an 710072, PR China.
authors.forename: Ping
authors.initials: P
authors.lastname: Guan
authors.affiliation: School of Natural and Applied Science, Northwestern Polytechnical University, Xi'an 710072, PR China.
authors.forename: Ji
authors.initials: J
authors.lastname: Li
authors.affiliation: School of Natural and Applied Science, Northwestern Polytechnical University, Xi'an 710072, PR China.
authors.forename: Na
authors.initials: N
authors.lastname: Zhao
authors.affiliation: School of Natural and Applied Science, Northwestern Polytechnical University, Xi'an 710072, PR China.
authors.forename: Qiaoli
authors.initials: Q
authors.lastname: Wang
mesh_terms: D000327:Adsorption; D002851:Chromatography, High Pressure Liquid; D005978:Glutathione / Q000652:urine*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D054802:Molecular Imprinting; D015394:Molecular Structure; D058105:Polymerization; D011108:Polymers / Q000737:chemistry; D052616:Solid Phase Extraction; D014466:Ultraviolet Rays
chemical_list: D011108:Polymers; D005978:Glutathione
keywords: Glutathione; Living radical polymerization; Molecularly imprinted polymer; Selectivity extraction; Solid-phase extraction