title: [Analysis of serum lipid profiles in guinea pigs for early detection of changes in metabolism under exposure to environmental contaminants].
abstract: The exposure to anthropogenic factors has a multifaceted impact on the body of humans and animals. Due to their complex influence the detection of negative effects of the certain factors is a rather complicated task. Metabolomic methodology which permits to overcome these difficulties, has been applied in the evaluation of the nature and degree of the impact of potash fertilizers production waste on lipid profiles of experimental animals after intranasal inoculation with potassic fertilizer production waste and consumption of drinking water obtained from sources located in the zone of potential action of potassic fertilizer production. Isolation of lipids from serum was performed with the help of specially developed technique based on solid-phase extraction of samples which allows to remove cholesterin from the samples. Each sample was subjected to HPLC -MS analysis, after which the obtained chromatograms were treated with the use of the method of principal component analysis and cluster analysis. The developed technique allows to efficiently separate hydrophobic metabolites in blood serum. There was established serum lipid profile of experimental animals, in particular the content of phospholipids and oxysteroids, and there were found differences in the metabolic processes of the test and control animals. It is shown that in the serum of experimental animals, there is observed an increased concentration of hydroxysteroid as compared with the control group.
authors.forename: P A
authors.initials: PA
authors.lastname: Chakhovskiy
authors.forename: A V
authors.initials: AV
authors.lastname: Yantsevich
authors.forename: A E
authors.initials: AE
authors.lastname: Dmitrochenko
authors.forename: A V
authors.initials: AV
authors.lastname: Ivanchik
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid; D016000:Cluster Analysis; D045424:Complex Mixtures / Q000737:chemistry / Q000633:toxicity; D060766:Drinking Water / Q000737:chemistry; D004781:Environmental Exposure / Q000009:adverse effects*; D004785:Environmental Pollutants / Q000737:chemistry / Q000633:toxicity*; D005260:Female; D005308:Fertilizers / Q000633:toxicity; D006168:Guinea Pigs; D008055:Lipids / Q000097:blood*; D008297:Male; D013058:Mass Spectrometry; D055432:Metabolomics / Q000379:methods; D010743:Phospholipids / Q000097:blood; D025341:Principal Component Analysis
chemical_list: D045424:Complex Mixtures; D060766:Drinking Water; D004785:Environmental Pollutants; D005308:Fertilizers; D008055:Lipids; D010743:Phospholipids; C518454:potash