title: Seroprevalence of neutralizing antibodies against dengue virus in two localities in the state of Morelos, Mexico.
abstract: Humoral immune response against dengue virus (DENV) is an important component in dengue-endemic transmission. We conducted a cross-sectional nested cohort study to determine the seroprevalence and frequency of neutralizing antibodies against DENV serotypes in two endemic localities in the state of Morelos, Mexico. The cohort participants (N = 1,196) were screened to determine previous exposure to DENV. Overall seroprevalence was 76.6% (95% confidence interval [95% CI] = 73.6-79.2), and prevalence of neutralizing antibodies in the 5- to 9-year-old group was 82.5% (95% CI = 67.2-92.7), 45% (95% CI = 29.3-61.5), and 65% (95% CI = 48.3-79.4) for DENV-1, DENV-2, and DENV-3, respectively. For participants older than 10 years, the observed seroprevalence was above 60% for each serotype, except DENV-4 in the 10- to 25-year-old group (42.9%); 81% of humoral responses were multitypic. The outcomes of our study contribute to understanding the immune component of dengue transmission and provide focal information for the evaluation of vaccine candidates under development.
authors.affiliation: Centro de Investigaci?n Sobre Enfermedades Infecciosas, Instituto Nacional de Salud Publica, Cuernavaca, Morelos, Mexico; Organizacion Latinoamericana para el Fomento de la Investigacion en Salud, Bucaramanga, Stder, Colombia; Department of Pathology and Center for Biodefense and Emerging Infectious Diseases, University of Texas Medical Branch, Galveston, Texas; Center for Tropical Diseases, University of Texas Medical Branch, Galveston, Texas; Institute of Human Infections and Immunity, University of Texas Medical Branch, Galveston, Texas.
authors.forename: Irma Y
authors.initials: IY
authors.lastname: Amaya-Larios
authors.affiliation: Centro de Investigaci?n Sobre Enfermedades Infecciosas, Instituto Nacional de Salud Publica, Cuernavaca, Morelos, Mexico; Organizacion Latinoamericana para el Fomento de la Investigacion en Salud, Bucaramanga, Stder, Colombia; Department of Pathology and Center for Biodefense and Emerging Infectious Diseases, University of Texas Medical Branch, Galveston, Texas; Center for Tropical Diseases, University of Texas Medical Branch, Galveston, Texas; Institute of Human Infections and Immunity, University of Texas Medical Branch, Galveston, Texas.
authors.forename: Ruth Aralí
authors.initials: RA
authors.lastname: Martínez-Vega
authors.affiliation: Centro de Investigaci?n Sobre Enfermedades Infecciosas, Instituto Nacional de Salud Publica, Cuernavaca, Morelos, Mexico; Organizacion Latinoamericana para el Fomento de la Investigacion en Salud, Bucaramanga, Stder, Colombia; Department of Pathology and Center for Biodefense and Emerging Infectious Diseases, University of Texas Medical Branch, Galveston, Texas; Center for Tropical Diseases, University of Texas Medical Branch, Galveston, Texas; Institute of Human Infections and Immunity, University of Texas Medical Branch, Galveston, Texas.
authors.forename: Sandra V
authors.initials: SV
authors.lastname: Mayer
authors.affiliation: Centro de Investigaci?n Sobre Enfermedades Infecciosas, Instituto Nacional de Salud Publica, Cuernavaca, Morelos, Mexico; Organizacion Latinoamericana para el Fomento de la Investigacion en Salud, Bucaramanga, Stder, Colombia; Department of Pathology and Center for Biodefense and Emerging Infectious Diseases, University of Texas Medical Branch, Galveston, Texas; Center for Tropical Diseases, University of Texas Medical Branch, Galveston, Texas; Institute of Human Infections and Immunity, University of Texas Medical Branch, Galveston, Texas.
authors.forename: Marisol
authors.initials: M
authors.lastname: Galeana-Hernández
authors.affiliation: Centro de Investigaci?n Sobre Enfermedades Infecciosas, Instituto Nacional de Salud Publica, Cuernavaca, Morelos, Mexico; Organizacion Latinoamericana para el Fomento de la Investigacion en Salud, Bucaramanga, Stder, Colombia; Department of Pathology and Center for Biodefense and Emerging Infectious Diseases, University of Texas Medical Branch, Galveston, Texas; Center for Tropical Diseases, University of Texas Medical Branch, Galveston, Texas; Institute of Human Infections and Immunity, University of Texas Medical Branch, Galveston, Texas.
authors.forename: Andreu
authors.initials: A
authors.lastname: Comas-García
authors.affiliation: Centro de Investigaci?n Sobre Enfermedades Infecciosas, Instituto Nacional de Salud Publica, Cuernavaca, Morelos, Mexico; Organizacion Latinoamericana para el Fomento de la Investigacion en Salud, Bucaramanga, Stder, Colombia; Department of Pathology and Center for Biodefense and Emerging Infectious Diseases, University of Texas Medical Branch, Galveston, Texas; Center for Tropical Diseases, University of Texas Medical Branch, Galveston, Texas; Institute of Human Infections and Immunity, University of Texas Medical Branch, Galveston, Texas.
authors.forename: Karla J
authors.initials: KJ
authors.lastname: Sepúlveda-Salinas
authors.affiliation: Centro de Investigaci?n Sobre Enfermedades Infecciosas, Instituto Nacional de Salud Publica, Cuernavaca, Morelos, Mexico; Organizacion Latinoamericana para el Fomento de la Investigacion en Salud, Bucaramanga, Stder, Colombia; Department of Pathology and Center for Biodefense and Emerging Infectious Diseases, University of Texas Medical Branch, Galveston, Texas; Center for Tropical Diseases, University of Texas Medical Branch, Galveston, Texas; Institute of Human Infections and Immunity, University of Texas Medical Branch, Galveston, Texas.
authors.forename: Jorge A
authors.initials: JA
authors.lastname: Falcón-Lezama
authors.affiliation: Centro de Investigaci?n Sobre Enfermedades Infecciosas, Instituto Nacional de Salud Publica, Cuernavaca, Morelos, Mexico; Organizacion Latinoamericana para el Fomento de la Investigacion en Salud, Bucaramanga, Stder, Colombia; Department of Pathology and Center for Biodefense and Emerging Infectious Diseases, University of Texas Medical Branch, Galveston, Texas; Center for Tropical Diseases, University of Texas Medical Branch, Galveston, Texas; Institute of Human Infections and Immunity, University of Texas Medical Branch, Galveston, Texas.
authors.forename: Nikos
authors.initials: N
authors.lastname: Vasilakis
authors.affiliation: Centro de Investigaci?n Sobre Enfermedades Infecciosas, Instituto Nacional de Salud Publica, Cuernavaca, Morelos, Mexico; Organizacion Latinoamericana para el Fomento de la Investigacion en Salud, Bucaramanga, Stder, Colombia; Department of Pathology and Center for Biodefense and Emerging Infectious Diseases, University of Texas Medical Branch, Galveston, Texas; Center for Tropical Diseases, University of Texas Medical Branch, Galveston, Texas; Institute of Human Infections and Immunity, University of Texas Medical Branch, Galveston, Texas jramos@insp.mx.
authors.forename: José
authors.initials: J
authors.lastname: Ramos-Castañeda
mesh_terms: D000293:Adolescent; D000328:Adult; D057134:Antibodies, Neutralizing / Q000097:blood*; D000914:Antibodies, Viral / Q000097:blood*; D002648:Child; D002675:Child, Preschool; D015331:Cohort Studies; D003430:Cross-Sectional Studies; D003715:Dengue / Q000453:epidemiology* / Q000276:immunology; D003716:Dengue Virus / Q000302:isolation & purification*; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D008297:Male; D008800:Mexico / Q000453:epidemiology; D016036:Seroepidemiologic Studies; D012703:Serotyping; D055815:Young Adult
chemical_list: D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D007074:Immunoglobulin G