title: Canonical histone H2Ba and H2A.X dimerize in an opposite genomic localization to H2A.Z/H2B.Z dimers in Toxoplasma gondii.
abstract: Histone H2Ba of Toxoplasma gondii was expressed as recombinant protein (rH2Ba) and used to generate antibody in mouse that is highly specific. Antibody recognizing rH2Ba detects a single band in tachyzoite lysate of the expected molecular weight (12kDa). By indirect immunofluorescence (IFA) in in vitro grown tachyzoites and bradyzoites, the signal was detected only in the parasite nucleus. The nucleosome composition of H2Ba was determined through co-immunoprecipitation assays. H2Ba was detected in the same immunocomplex as H2A.X, but not with H2A.Z. Through chromatin immunoprecipitation (ChIP) assays and qPCR, it was observed that H2Ba is preferentially located at promoters of inactive genes and silent regions, accompanying H2A.X and opposed to H2A.Z/H2B.Z dimers.
authors.affiliation: Laboratorio de Parasitología Molecular, IIB-INTECH, CONICET-UNSAM, Av. Intendente Marino Km. 8.2, C.C 164, (B7130IIWA), Chascomús, Prov. Buenos Aires, Argentina.
authors.forename: Silvina S
authors.initials: SS
authors.lastname: Bogado
authors.affiliation: Laboratorio de Parasitología Molecular, IIB-INTECH, CONICET-UNSAM, Av. Intendente Marino Km. 8.2, C.C 164, (B7130IIWA), Chascomús, Prov. Buenos Aires, Argentina.
authors.forename: María C
authors.initials: MC
authors.lastname: Dalmasso
authors.affiliation: Laboratorio de Parasitología Molecular, IIB-INTECH, CONICET-UNSAM, Av. Intendente Marino Km. 8.2, C.C 164, (B7130IIWA), Chascomús, Prov. Buenos Aires, Argentina; Scientific Research Commission (CIC, Buenos Aires), Argentina.
authors.forename: Agustina
authors.initials: A
authors.lastname: Ganuza
authors.affiliation: Department of Microbiology & Immunology, Albert Einstein College of Medicine, Bronx, NY, USA; Department of Pathology, Albert Einstein College of Medicine, Bronx, NY, USA; Department of Medicine, Albert Einstein College of Medicine, Bronx, NY, USA.
authors.forename: Kami
authors.initials: K
authors.lastname: Kim
authors.affiliation: Department of Pharmacology & Toxicology, Indiana University School of Medicine, Indianapolis, IN, USA; Department of Microbiology & Immunology, Indiana University School of Medicine, Indianapolis, IN, USA.
authors.forename: William J
authors.initials: WJ
authors.lastname: Sullivan
authors.affiliation: Laboratorio de Parasitología Molecular, IIB-INTECH, CONICET-UNSAM, Av. Intendente Marino Km. 8.2, C.C 164, (B7130IIWA), Chascomús, Prov. Buenos Aires, Argentina.
authors.forename: Sergio O
authors.initials: SO
authors.lastname: Angel
authors.affiliation: Laboratorio de Parasitología Molecular, IIB-INTECH, CONICET-UNSAM, Av. Intendente Marino Km. 8.2, C.C 164, (B7130IIWA), Chascomús, Prov. Buenos Aires, Argentina. Electronic address: vanagas@intech.gov.ar.
authors.forename: Laura
authors.initials: L
authors.lastname: Vanagas
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology; D047369:Chromatin Immunoprecipitation; D018503:Genome, Protozoan; D006657:Histones / Q000737:chemistry / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D008954:Models, Biological; D009707:Nucleosomes / Q000378:metabolism; D055503:Protein Multimerization; D021381:Protein Transport; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D014122:Toxoplasma / Q000235:genetics*
chemical_list: D000911:Antibodies, Monoclonal; D006657:Histones; D009707:Nucleosomes; D011994:Recombinant Proteins
keywords: Canonical histones; ChIP; Chromatin; Epigenetics; Nucleosome; Toxoplasma