title: Human papillomavirus detection in paraffin-embedded colorectal cancer tissues.
abstract: Human papillomavirus (HPV) has a well-recognized aetiological role in the development of cervical cancer and other anogenital tumours. Recently, an association between colorectal cancer and HPV infection has been suggested, although this is still controversial. This study aimed at detecting and characterizing HPV infection in 57 paired biopsies from colorectal cancers and adjacent intact tissues using a degenerate PCR approach. All amplified fragments were genotyped by means of sequencing. Overall, HPV prevalence was 12.3 %. In particular, 15.8 % of tumour tissues and 8.8 % of non-cancerous tissue samples were HPV DNA-positive. Of these samples, 85.7 % were genotyped successfully, with 41.7 % of sequences identifying four genotypes of the HR (high oncogenic risk) clade Group 1; the remaining 58.3 % of HPV-genotyped specimens had an unclassified β-HPV. Examining additional cases and analysing whole genomes will help to outline the significance of these findings.
authors.affiliation: Department of Biomedical Sciences for Health, University of Milan, Milan, Italy.
authors.forename: Elisabetta
authors.initials: E
authors.lastname: Tanzi
authors.affiliation: Department of Biomedical Sciences for Health, University of Milan, Milan, Italy.
authors.forename: Silvia
authors.initials: S
authors.lastname: Bianchi
authors.affiliation: Department of Biomedical Sciences for Health, University of Milan, Milan, Italy.
authors.forename: Elena R
authors.initials: ER
authors.lastname: Frati
authors.affiliation: Department of Health Sciences, University of Genoa, Genoa, Italy.
authors.forename: Daniela
authors.initials: D
authors.lastname: Amicizia
authors.affiliation: Department of Biomedical Sciences for Health, University of Milan, Milan, Italy.
authors.forename: Marianna
authors.initials: M
authors.lastname: Martinelli
authors.affiliation: Department of Health Sciences, University of Genoa, Genoa, Italy.
authors.forename: Nicola L
authors.initials: NL
authors.lastname: Bragazzi
authors.affiliation: Pathological Anatomy and Histology Unit, IRCCS AOU San Martino-IST, Genoa, Italy.
authors.forename: Maria Pia
authors.initials: MP
authors.lastname: Brisigotti
authors.affiliation: Department of Biomedical Sciences for Health, University of Milan, Milan, Italy.
authors.forename: Daniela
authors.initials: D
authors.lastname: Colzani
authors.affiliation: Department of Biomedical Sciences for Health, University of Milan, Milan, Italy.
authors.forename: Ester
authors.initials: E
authors.lastname: Fasoli
authors.affiliation: Department of Biomedical and Clinical Sciences 'L. Sacco', University of Milan, Milan, Italy.
authors.forename: Gianguglielmo
authors.initials: G
authors.lastname: Zehender
authors.affiliation: Department of Health Sciences, University of Genoa, Genoa, Italy.
authors.forename: Donatella
authors.initials: D
authors.lastname: Panatto
authors.affiliation: Department of Health Sciences, University of Genoa, Genoa, Italy.
authors.forename: Roberto
authors.initials: R
authors.lastname: Gasparini
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015179:Colorectal Neoplasms / Q000175:diagnosis* / Q000821:virology*; D004279:DNA, Viral / Q000235:genetics; D005260:Female; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D027383:Papillomaviridae / Q000235:genetics*; D030361:Papillomavirus Infections / Q000175:diagnosis* / Q000821:virology*; D010232:Paraffin / Q000737:chemistry*
chemical_list: D004279:DNA, Viral; D010232:Paraffin