title: In vivo genotoxic effects of four different nano-sizes forms of silica nanoparticles in Drosophila melanogaster.
abstract: Although the use of synthetic amorphous silica (SAS) is steady increasing, scarce information exists on its potential health risk. In particular few and conflictive data exist on its genotoxicity. To fill in this gap we have used Drosophila melanogaster as in vivo model test organism to detect the genotoxic activity of different SAS with different primary sizes (6, 15, 30 and 55 nm). The wing-spot assay and the comet assay in larvae haemocytes were used, and the obtained results were compared with those obtained with the microparticulated form (silicon dioxide). All compounds were administered to third instar larvae at concentrations ranging from 0.1 to 10mM. No significant increases in the frequencies of mutant spots were observed in the wing-spot assay with any of the tested compounds. On the other hand, significant dose-dependent increases in the levels of primary DNA damage, measured by the comet assay, were observed for all the SAS evaluated but mainly when high doses (5 and 10mM) were used. These in vivo results contribute to increase the database dealing with the potential genotoxic risk associated to SAS nanoparticles exposure.
authors.affiliation: Akdeniz University, Faculty of Sciences, Department of Biology, 07058-Campus Antalya, Turkey.
authors.forename: Eşref
authors.initials: E
authors.lastname: Demir
authors.affiliation: Akdeniz University, Faculty of Sciences, Department of Biology, 07058-Campus Antalya, Turkey.
authors.forename: Sezgin
authors.initials: S
authors.lastname: Aksakal
authors.affiliation: Akdeniz University, Faculty of Sciences, Department of Biology, 07058-Campus Antalya, Turkey.
authors.forename: Fatma
authors.initials: F
authors.lastname: Turna
authors.affiliation: Akdeniz University, Faculty of Sciences, Department of Biology, 07058-Campus Antalya, Turkey.
authors.forename: Bülent
authors.initials: B
authors.lastname: Kaya
authors.affiliation: Grup de Mutagènesi, Departament de Genètica i de Microbiologia, Facultat de Biociències, Universitat Autònoma de Barcelona, Campus de Bellaterra, Cerdanyola del Vallès, 08193 Barcelona, Spain; CIBER Epidemiología y Salud Pública, ISCIII, Madrid, Spain. Electronic address: ricard.marcos@uab.es.
authors.forename: Ricard
authors.initials: R
authors.lastname: Marcos
mesh_terms: D000818:Animals; D020552:Comet Assay; D004331:Drosophila melanogaster; D015386:Hazardous Substances / Q000633:toxicity*; D009152:Mutagenicity Tests; D053758:Nanoparticles / Q000633:toxicity*; D010316:Particle Size; D012822:Silicon Dioxide / Q000633:toxicity*
chemical_list: D015386:Hazardous Substances; D012822:Silicon Dioxide
keywords: Alkaline comet assay; Drosophila melanogaster; Genotoxicity; SAS nanoparticles; Wing spot assay