title: Suprabasin as a novel tumor endothelial cell marker.
abstract: Recent studies have reported that stromal cells contribute to tumor progression. We previously demonstrated that tumor endothelial cells (TEC) characteristics were different from those of normal endothelial cells (NEC). Furthermore, we performed gene profile analysis in TEC and NEC, revealing that suprabasin (SBSN) was upregulated in TEC compared with NEC. However, its role in TEC is still unknown. Here we showed that SBSN expression was higher in isolated human and mouse TEC than in NEC. SBSN knockdown inhibited the migration and tube formation ability of TEC. We also showed that the AKT pathway was a downstream factor of SBSN. These findings suggest that SBSN is involved in the angiogenic potential of TEC and may be a novel TEC marker.
authors.affiliation: Vascular Biology, Frontier Research Unit, Institute for Genetic Medicine, Hokkaido University, Sapporo, Japan; Department of Oral Pathology and Biology, Graduate School of Dental Medicine, Hokkaido University, Sapporo, Japan.
authors.forename: Mohammad T
authors.initials: MT
authors.lastname: Alam
authors.forename: Hiroko
authors.initials: H
authors.lastname: Nagao-Kitamoto
authors.forename: Noritaka
authors.initials: N
authors.lastname: Ohga
authors.forename: Kosuke
authors.initials: K
authors.lastname: Akiyama
authors.forename: Nako
authors.initials: N
authors.lastname: Maishi
authors.forename: Taisuke
authors.initials: T
authors.lastname: Kawamoto
authors.forename: Nobuo
authors.initials: N
authors.lastname: Shinohara
authors.forename: Akinobu
authors.initials: A
authors.lastname: Taketomi
authors.forename: Masanobu
authors.initials: M
authors.lastname: Shindoh
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Hida
authors.forename: Kyoko
authors.initials: K
authors.lastname: Hida
mesh_terms: D000818:Animals; D000943:Antigens, Differentiation / Q000235:genetics / Q000378:metabolism*; D014408:Biomarkers, Tumor / Q000378:metabolism; D045744:Cell Line, Tumor; D042783:Endothelial Cells / Q000378:metabolism / Q000473:pathology*; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D055785:Gene Knockdown Techniques; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D009362:Neoplasm Metastasis / Q000473:pathology; D009363:Neoplasm Proteins / Q000235:genetics / Q000378:metabolism*; D009369:Neoplasms / Q000378:metabolism / Q000473:pathology*; D009389:Neovascularization, Pathologic / Q000378:metabolism; D015398:Signal Transduction
chemical_list: D000943:Antigens, Differentiation; D014408:Biomarkers, Tumor; D009363:Neoplasm Proteins; C579316:SBSN protein, human; C470006:suprabasin protein, mouse
keywords: Angiogenesis; suprabasin; suprabasin signaling; tumor endothelial cell marker; tumor endothelial cells