title: Modification of vectors for functional genomic analysis in plants.
abstract: Simple, efficient, and economical recombinant plant binary expression vectors for deciphering large-scale functional genomic research in plants and promoting crop improvement by genetically engineering and biotechnology is in great demand. In this research, using the pCHF3, pCAMBIA1301, pCAMBIA3300, pCAMBIA3301 vectors, we successfully constructed general plant binary expression vectors carrying CaMV35S and Arabidopsis rd29A promoters mediating multiple cloning sites (
  SacI, KpnI, SmaI, BamHI, XbaI, SalI, and PstI). Meanwhile, a series of applicative binary expression vectors that can be utilized for subcellular localization were constructed by fusion of the MCS and eGFP. Subsequently, the recombinant vectors were successfully transferred into Arabidopsis thaliana and Nicotiana benthamiana for further investigation of functional elements in these plant binary expression vectors. Our results demonstrated that this system was a convenient and versatile vector system for phenotypic, functional, subcellular localization, and promoter activity analysis, and it provided a relatively high-efficiency and reliable platform for researchers in vector construction and may facilitate large-scale functional genomics analysis in plants.
authors.affiliation: College of Plant Science, Jilin University, Changchun, China.
authors.forename: J T
authors.initials: JT
authors.lastname: Li
authors.affiliation: College of Plant Science, Jilin University, Changchun, China.
authors.forename: G
authors.initials: G
authors.lastname: Yu
authors.affiliation: College of Plant Science, Jilin University, Changchun, China.
authors.forename: X H
authors.initials: XH
authors.lastname: Sun
authors.affiliation: College of Plant Science, Jilin University, Changchun, China.
authors.forename: C G
authors.initials: CG
authors.lastname: Jia
authors.affiliation: Jilin Academy of Agricultural Science, Changchun, China.
authors.forename: Q
authors.initials: Q
authors.lastname: Du
authors.affiliation: Jilin Academy of Agricultural Science, Changchun, China.
authors.forename: Q Y
authors.initials: QY
authors.lastname: Li
authors.affiliation: College of Plant Science, Jilin University, Changchun, China panhongyu@jlu.edu.cn.
authors.forename: H Y
authors.initials: HY
authors.lastname: Pan
mesh_terms: D001483:Base Sequence; D017931:DNA Primers; D017930:Genes, Reporter; D005822:Genetic Vectors; D018745:Genome, Plant; D005966:Glucuronidase / Q000235:genetics; D049452:Green Fluorescent Proteins / Q000235:genetics; D016133:Polymerase Chain Reaction; D013347:Subcellular Fractions / Q000378:metabolism
chemical_list: D017931:DNA Primers; D049452:Green Fluorescent Proteins; D005966:Glucuronidase