title: Experimental Mycobacterium tuberculosis infection in the Chinese tree shrew.
abstract: In recent years, the Chinese tree shrew has been considered to be a promising experimental animal for numerous diseases. Yet the susceptibility of Mycobacterium tuberculosis (MTB) in Chinese tree shrew is still unknown. We infected Chinese tree shrews with a high dose (2.5 × 10(6) CFU) or a low dose (2.5 × 10(3) CFU) of the H37Rv strain via the femoral vein to cause severe or mild disease. Disease severity was determined by clinical signs, pathologic changes and bacteria distribution in organs. Furthermore, among lung samples of the uninfected, mildly and seriously ill Chinese tree shrews, differentially expressed protein profiles were analyzed through iTRAQ and validated by qPCR. Tuberculous nodules, skin ulceration, pleural effusion and cerebellum necrosis could be observed in seriously ill animals. Regulation of the actin cytoskeleton was newly defined as a possible MTB-related pathway correlated with disease progression. This comprehensive analysis of the experimental infection and the depiction of the proteomics profiles in the Chinese tree shrew provide a foundation for the establishment of a new animal model of tuberculosis and provide a better understanding of the mechanism of tuberculosis.
authors.affiliation: Key Laboratory of Human Diseases Comparative Medicine, Ministry of Health, Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences and Comparative Medicine Center, Peking Union Medical College, Beijing, China.
authors.forename: Lingjun
authors.initials: L
authors.lastname: Zhan
authors.forename: Hairong
authors.initials: H
authors.lastname: Ding
authors.forename: Shuzhu
authors.initials: S
authors.lastname: Lin
authors.forename: Jun
authors.initials: J
authors.lastname: Tang
authors.forename: Wei
authors.initials: W
authors.lastname: Deng
authors.forename: Yanfeng
authors.initials: Y
authors.lastname: Xu
authors.forename: Yuhuan
authors.initials: Y
authors.lastname: Xu
authors.forename: Chuan
authors.initials: C
authors.lastname: Qin
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D008168:Lung / Q000737:chemistry / Q000382:microbiology; D008297:Male; D009169:Mycobacterium tuberculosis; D011506:Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D020543:Proteome / Q000032:analysis; D015398:Signal Transduction; D014376:Tuberculosis / Q000378:metabolism / Q000382:microbiology / Q000473:pathology / Q000503:physiopathology; D014418:Tupaiidae / Q000382:microbiology*
chemical_list: D011506:Proteins; D020543:Proteome
keywords: Chinese tree shrew; Mycobacterium tuberculosis; pathologic change; proteomics; signal pathway