title: Boron containing poly-(lactide-co-glycolide) (PLGA) scaffolds for bone tissue engineering.
abstract: Scaffold-based bone defect reconstructions still face many challenges due to their inadequate osteoinductive and osteoconductive properties. Various biocompatible and biodegradable scaffolds, combined with proper cell type and biochemical signal molecules, have attracted significant interest in hard tissue engineering approaches. In the present study, we have evaluated the effects of boron incorporation into poly-(lactide-co-glycolide-acid) (PLGA) scaffolds, with or without rat adipose-derived stem cells (rADSCs), on bone healing in vitro and in vivo. The results revealed that boron containing scaffolds increased in vitro proliferation, attachment and calcium mineralization of rADSCs. In addition, boron containing scaffold application resulted in increased bone regeneration by enhancing osteocalcin, VEGF and collagen type I protein levels in a femur defect model. Bone mineralization density (BMD) and computed tomography (CT) analysis proved that boron incorporated scaffold administration increased the healing rate of bone defects. Transplanting stem cells into boron containing scaffolds was found to further improve bone-related outcomes compared to control groups. Additional studies are highly warranted for the investigation of the mechanical properties of these scaffolds in order to address their potential use in clinics. The study proposes that boron serves as a promising innovative approach in manufacturing scaffold systems for functional bone tissue engineering.
authors.affiliation: Department of Genetics and Bioengineering, Faculty of Engineering and Architecture, Yeditepe University 34755 Istanbul, Turkey.
authors.forename: Ayşegül
authors.initials: A
authors.lastname: Doğan
authors.affiliation: Department of Genetics and Bioengineering, Faculty of Engineering and Architecture, Yeditepe University 34755 Istanbul, Turkey.
authors.forename: Selami
authors.initials: S
authors.lastname: Demirci
authors.affiliation: Department of Biochemistry, Faculty of Pharmacy, Ataturk University, 25240, Erzurum, Turkey.
authors.forename: Yasin
authors.initials: Y
authors.lastname: Bayir
authors.affiliation: Department of Pharmacology, Faculty of Medicine, Ataturk University, 25240, Erzurum, Turkey.
authors.forename: Zekai
authors.initials: Z
authors.lastname: Halici
authors.affiliation: Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, Ataturk University, 25240, Erzurum, Turkey.
authors.forename: Emre
authors.initials: E
authors.lastname: Karakus
authors.affiliation: Department of Orthopedics and Traumatology, Faculty of Medicine, Ataturk University, 25240, Erzurum, Turkey.
authors.forename: Ali
authors.initials: A
authors.lastname: Aydin
authors.affiliation: Department of Pharmacology, Faculty of Pharmacy, Ataturk University, 25240, Erzurum, Turkey.
authors.forename: Elif
authors.initials: E
authors.lastname: Cadirci
authors.affiliation: Department of Pharmacology, Faculty of Medicine, Ataturk University, 25240, Erzurum, Turkey.
authors.forename: Abdulmecit
authors.initials: A
authors.lastname: Albayrak
authors.affiliation: Department of Pathology, Faculty of Medicine, Ataturk University, 25240, Erzurum, Turkey.
authors.forename: Elif
authors.initials: E
authors.lastname: Demirci
authors.affiliation: Department of Radiology, Faculty of Medicine, Ataturk University, 25240, Erzurum, Turkey.
authors.forename: Adem
authors.initials: A
authors.lastname: Karaman
authors.affiliation: Department of Nuclear Medicine, Faculty of Medicine, Ataturk University, 25240, Erzurum, Turkey.
authors.forename: Arif Kursat
authors.initials: AK
authors.lastname: Ayan
authors.affiliation: Department of Pathology, Faculty of Medicine, Ataturk University, 25240, Erzurum, Turkey.
authors.forename: Cemal
authors.initials: C
authors.lastname: Gundogdu
authors.affiliation: Department of Genetics and Bioengineering, Faculty of Engineering and Architecture, Yeditepe University 34755 Istanbul, Turkey. Electronic address: fsahin@yeditepe.edu.tr.
authors.forename: Fikrettin
authors.initials: F
authors.lastname: Sahin
mesh_terms: D015502:Absorptiometry, Photon; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D001861:Bone Regeneration; D001842:Bone and Bones / Q000187:drug effects / Q000378:metabolism; D001895:Boron / Q000737:chemistry* / Q000494:pharmacology; D002113:Calcification, Physiologic; D002470:Cell Survival / Q000187:drug effects; D024042:Collagen Type I / Q000378:metabolism; D007150:Immunohistochemistry; D019344:Lactic Acid / Q000737:chemistry* / Q000494:pharmacology; D008855:Microscopy, Electron, Scanning; D015675:Osteocalcin / Q000378:metabolism; D011100:Polyglycolic Acid / Q000737:chemistry* / Q000494:pharmacology; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D051381:Rats; D017207:Rats, Sprague-Dawley; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials; D024042:Collagen Type I; D015675:Osteocalcin; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D001895:Boron
keywords: Adipose-derived stem cells; Bone tissue engineering; Boron; PLGA; Scaffold