title: Lipid production by a mixed culture of oleaginous yeast and microalga from distillery and domestic mixed wastewater.
abstract: Lipid productivity by mixed culture of Rhodosporidium toruloides and Chlorella pyrenoidosa was studied using 1:1 mixed real wastewater from distillery and local municipal wastewater treatment plant with initial soluble chemical oxygen demand (SCOD) around 25,000 mg/L, initial cell density of 2×10(7) cells/mL (yeast) and 5×10(6) cells/mL (microalga), at 30 °C and 2.93 W/m2 (2000 lux, 12:12 h light and dark cycles). Lipid content and lipid yield achieved were 63.45±2.58% and 4.60±0.36 g/L with the associated removal efficiencies for SCOD, total nitrogen (TN), and total phosphorus (TP) at 95.34±0.07%, 51.18±2.17%, and 89.29±4.91%, respectively, after 5 days of cultivation without the pH adjustment. Inoculation of microalgae at 40 h of the initial yeast cultivation and harvesting part of inactive biomass at 72 h by sedimentation could improve both lipid production and wastewater treatment efficiency under non-sterile conditions.
authors.affiliation: Department of Civil and Environmental Engineering, Faculty of Science and Technology, University of Macau, Taipa, Macau SAR, China.
authors.forename: Jiayin
authors.initials: J
authors.lastname: Ling
authors.affiliation: Department of Civil and Environmental Engineering, Faculty of Science and Technology, University of Macau, Taipa, Macau SAR, China.
authors.forename: Saiwa
authors.initials: S
authors.lastname: Nip
authors.affiliation: Department of Civil and Environmental Engineering, Faculty of Science and Technology, University of Macau, Taipa, Macau SAR, China.
authors.forename: Wai Leong
authors.initials: WL
authors.lastname: Cheok
authors.affiliation: Department of Civil and Environmental Engineering, Faculty of Science and Technology, University of Macau, Taipa, Macau SAR, China.
authors.forename: Renata Alves
authors.initials: RA
authors.lastname: de Toledo
authors.affiliation: Department of Civil and Environmental Engineering, Faculty of Science and Technology, University of Macau, Taipa, Macau SAR, China. Electronic address: hjshim@umac.mo.
authors.forename: Hojae
authors.initials: H
authors.lastname: Shim
mesh_terms: D001487:Basidiomycota / Q000145:classification / Q000502:physiology*; D001673:Biodegradation, Environmental; D018920:Coculture Techniques / Q000379:methods; D007220:Industrial Waste / Q000517:prevention & control; D050356:Lipid Metabolism / Q000502:physiology*; D008055:Lipids / Q000302:isolation & purification; D058086:Microalgae / Q000145:classification / Q000502:physiology*; D059013:Microbial Consortia / Q000502:physiology*; D013045:Species Specificity; D062065:Wastewater / Q000382:microbiology*; D014874:Water Pollutants, Chemical / Q000302:isolation & purification / Q000378:metabolism*; D018508:Water Purification / Q000379:methods*
chemical_list: D007220:Industrial Waste; D008055:Lipids; D062065:Wastewater; D014874:Water Pollutants, Chemical
keywords: Lipid production; Microalga; Mixed culture; Mixed wastewater; Oleaginous yeast