title: Lack of association between CD40 polymorphisms and acute rejection in German liver transplant recipients.
abstract: CD40 and its ligand, CD154, are major costimulatory molecules whose interactions are important in alloreactive transplant rejection. The aim of this study was to examine the association of CD40 polymorphisms with the susceptibility to acute rejection episodes in liver transplantation. In total, 112 liver transplant recipients with biopsy proven acute rejections (BPAR), 97 without BPAR (WBPAR), and 112 healthy control individuals were enrolled in the study. Two single nucleotide polymorphisms (SNPs) of CD40 gene (rs1883832 and rs4810485) were genotyped by polymerase chain reaction-allele specific restriction enzyme analysis (PCR-ASRA). Both SNPs has been tested for a recessive and a dominant model. No significant differences were found in the genotype and allele frequencies of the SNPs rs1883832 and rs4810485 between BPAR liver recipients and WBPAR recipients. Our results do not suggest an important role of tested CD40 SNPs in the susceptibility to acute liver transplant rejection in a Caucasian population.
authors.affiliation: University Medical Center Hamburg-Eppendorf, Department of Hepatobiliary and Transplant Surgery, Martinistraße 52, 20246 Hamburg, Germany.
authors.forename: Hansjörg
authors.initials: H
authors.lastname: Thude
authors.affiliation: University Medical Center Hamburg-Eppendorf, Department of Hepatobiliary and Transplant Surgery, Martinistraße 52, 20246 Hamburg, Germany.
authors.forename: Kathrin
authors.initials: K
authors.lastname: Kramer
authors.affiliation: University Medical Center Hamburg-Eppendorf, Department of Hepatobiliary and Transplant Surgery, Martinistraße 52, 20246 Hamburg, Germany.
authors.forename: Martina
authors.initials: M
authors.lastname: Koch
authors.affiliation: University Medical Center Hamburg-Eppendorf, Institute for Transfusion Medicine, Martinistraße 52, 20246 Hamburg, Germany.
authors.forename: Sven
authors.initials: S
authors.lastname: Peine
authors.affiliation: University Medical Center Hamburg-Eppendorf, Department of Medicine, Martinistraße 52, 20246 Hamburg, Germany.
authors.forename: Martina
authors.initials: M
authors.lastname: Sterneck
authors.affiliation: University Medical Center Hamburg-Eppendorf, Department of Hepatobiliary and Transplant Surgery, Martinistraße 52, 20246 Hamburg, Germany.
authors.forename: Björn
authors.initials: B
authors.lastname: Nashan
mesh_terms: D000208:Acute Disease; D000328:Adult; D000483:Alleles; D019013:CD40 Antigens / Q000235:genetics / Q000276:immunology*; D016022:Case-Control Studies; D005260:Female; D015870:Gene Expression; D005787:Gene Frequency; D005838:Genotype; D006084:Graft Rejection / Q000208:ethnology / Q000235:genetics / Q000276:immunology* / Q000473:pathology; D048550:Hepatic Insufficiency / Q000235:genetics / Q000276:immunology / Q000473:pathology / Q000601:surgery; D006801:Humans; D008099:Liver / Q000276:immunology / Q000473:pathology / Q000601:surgery; D016031:Liver Transplantation; D008297:Male; D008875:Middle Aged; D008957:Models, Genetic; D020641:Polymorphism, Single Nucleotide; D012189:Retrospective Studies; D044465:White People
chemical_list: D019013:CD40 Antigens
keywords: Acute liver transplant rejection; CD40 polymorphisms; Liver transplantation; Single nucleotide polymorphism rs1883832; Single nucleotide polymorphism rs4810485