title: Highly selective isolation and purification of heme proteins in biological samples using multifunctional magnetic nanospheres.
abstract: Magnetic particles with suitable surface modification are capable of binding proteins selectively, and magnetic separations have advantages of rapidity, convenience, and high selectivity. In this paper, new magnetic nanoparticles modified with imidazolium ionic liquid (Fe3O4 @SiO2 @ILs) were successfully fabricated. N-Methylimidazolium was immobilized onto silica-coated magnetic nanoparticles via γ-chloropropyl modification as a magnetic nanoadsorbent for heme protein separation. The particle size was about 90 nm without significant aggregation during the preparation process. Hemoglobin as one of heme proteins used in this experiment was compared with other nonheme proteins. It has been found that the magnetic nanoparticles can be used for more rapid, efficient, and specific adsorption of hemoglobin with a binding capacity as high as 5.78 mg/mg. In comparison with other adsorption materials of proteins in the previous reports, Fe3 O4 @SiO2 @ILs magnetic nanoparticles exhibit the excellent performance in isolation of heme proteins with higher binding capacity and selectivity. In addition, a short separation time makes the functionalized nanoparticles suitable for purifying unstable proteins, as well as having other potential applications in a variety of biomedical fields.
authors.affiliation: State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing, P. R. China.
authors.forename: Yating
authors.initials: Y
authors.lastname: Liu
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.forename: Yun
authors.initials: Y
authors.lastname: Wei
mesh_terms: D000818:Animals; D002417:Cattle; D002645:Chickens; D006454:Hemoglobins / Q000737:chemistry / Q000302:isolation & purification*; D006863:Hydrogen-Ion Concentration; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D015394:Molecular Structure; D009113:Muramidase / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D054118:Nanospheres / Q000737:chemistry*; D010316:Particle Size; D013499:Surface Properties
chemical_list: D006454:Hemoglobins; D058185:Magnetite Nanoparticles; D009113:Muramidase
keywords: Heme proteins; Ionic liquids; Magnetic particles; Nanospheres; Purification