title: Differential regulation of proteins in rice (Oryza sativa L.) under iron deficiency.
abstract: Sixty-three proteins were identified to be differentially accumulated due to iron deficiency in shoot and root. The importance of these proteins alterations on shoot physiology is discussed. Iron (Fe) is an essential micronutrient for plant growth and its accumulation affects the quality of edible plant organs. To investigate the adaptive mechanism of a Chinese rice variety grown under iron deficiency, proteins differentially accumulated in leaves and roots of Yangdao 6, an indica cultivar, under Fe deficiency growth condition, were profiled using a two-dimensional electrophoresis (2-DE) and matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF/MS). The accumulations of seventy-three proteins were detected to be increased or decreased upon iron deficiency, and sixty-three of them were successfully identified. Among the sixty-three proteins, a total of forty proteins were identified in rice leaves, and twenty-three proteins were in roots. Most of these proteins are involved in photosynthesis, C metabolism, oxidative stress, Adenosine triphosphate synthesis, cell growth or signal transduction. The results provide a comprehensive way to understand, at the level of proteins, the adaptive mechanism used by rice shoots and roots under iron deficiency.
authors.affiliation: Key Laboratory of Crop Physiology and Ecology in Southern China, College of Agriculture, Ministry of Agricultural University, Nanjing Agricultural University, Nanjing, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Chen
authors.forename: Chengqiang
authors.initials: C
authors.lastname: Ding
authors.forename: Xiufeng
authors.initials: X
authors.lastname: Zhao
authors.forename: Junxu
authors.initials: J
authors.lastname: Xu
authors.forename: Alim Abdul
authors.initials: AA
authors.lastname: Mohammad
authors.forename: Shaohua
authors.initials: S
authors.lastname: Wang
authors.forename: Yanfeng
authors.initials: Y
authors.lastname: Ding
mesh_terms: D015180:Electrophoresis, Gel, Two-Dimensional; D000090463:Iron Deficiencies; D012275:Oryza / Q000378:metabolism*; D018515:Plant Leaves / Q000378:metabolism; D010940:Plant Proteins / Q000145:classification / Q000378:metabolism*; D018517:Plant Roots / Q000378:metabolism; D020543:Proteome / Q000145:classification / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D010940:Plant Proteins; D020543:Proteome