title: Direct detection of chloramphenicol in honey by neutral desorption-extractive electrospray ionization mass spectrometry.
abstract: Herein, we constructed a platform of neutral desorption-extractive electrospray ionization mass spectrometry (ND-EESI-MS) for direct and rapid detection of chloramphenicol (CAP) in honey samples diluted with methanol. Under the optimized working conditions, the quantitative information of CAP residues was acquired effectively by EESI-Ion Trap MS (n) . Using heated methanol-N2 as spray reagent, we reduced the limit of determination (LOD) from 73.3 ng/mL to 0.3 ng/mL, and the CAP detection is linear in the range of 1-5000 ng/mL (R = 0.9947). For the honey samples with CAP of 10, 100, and 1000 ng/mL, the recoveries were 133.0, 80.6, and 101.1%, and the relative standard deviations were 5.96, 8.82, and 8.71%, respectively. The reproducibility assays showed the stability of this method. Therefore, this ND-EESI-MS method is powerful for direct, rapid, and quantitative CAP analysis in honey samples with high sensitivity, precision, and specificity.
authors.affiliation: State Key Laboratory of Food Science and Technology, School of Life Sciences and Food Engineering, Nanchang University, 999 Xuefu Road, Nanchang, Jiangxi, 330031, China.
authors.forename: X Y
authors.initials: XY
authors.lastname: Huang
authors.forename: X W
authors.initials: XW
authors.lastname: Fang
authors.forename: X
authors.initials: X
authors.lastname: Zhang
authors.forename: X M
authors.initials: XM
authors.lastname: Dai
authors.forename: X L
authors.initials: XL
authors.lastname: Guo
authors.forename: H W
authors.initials: HW
authors.lastname: Chen
authors.forename: L P
authors.initials: LP
authors.lastname: Luo
mesh_terms: D000327:Adsorption; D000900:Anti-Bacterial Agents / Q000032:analysis; D002701:Chloramphenicol / Q000032:analysis*; D004785:Environmental Pollutants / Q000032:analysis*; D005504:Food Analysis / Q000379:methods*; D005506:Food Contamination / Q000032:analysis*; D006722:Honey / Q000032:analysis*; D059625:Liquid-Liquid Extraction / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
chemical_list: D000900:Anti-Bacterial Agents; D004785:Environmental Pollutants; D002701:Chloramphenicol