title: Effects of ultra violet radiation on the soluble proteins of human hair.
abstract: Exposure of hair fibers from healthy volunteers to Ultra Violet Radiation (UVR) under laboratory conditions enhanced protein elution from the hair tresses into a buffer solution (pH 10.5). At the same time the UVR decreased the intensity of tryptophan fluorescence in the eluted proteins. After mechanical homogenization of these hair samples, the increase of soluble protein was registered for UVR treated hair as well as the rise in sulfhydryl group content of these proteins. Analysis of soluble proteins from hair samples homogenized before and after protein elution has shown that mainly proteins rich in sulfhydryl groups were eluted and as a result sulfhydryl content of proteins in hair shaft decreased. The hypothesis concerning the effects of environmental factors on the properties of hair shaft proteins was examined, the proximal and distal parts of normal hair (0-5 cm and 15-20 cm from hair root) were compared. In the distal parts there was a higher quantity of soluble proteins registered after homogenization, with decreased sulfhydryl group content and tryptophan fluorescence. It could be supposed that this difference results from the steady rupture of cystine in sulfur bridges and tryptophan under exposure to environmental factors (mainly, UVR), followed by elution of the resulting peptides.
authors.affiliation: Research Institute of Physico-Chemical Medicine, Malaja Pyrogovskaya 1-a, Moscow 119435, Russia. Electronic address: furba07@mail.ru.
authors.forename: M V
authors.initials: MV
authors.lastname: Fedorkova
authors.affiliation: Research Institute of Physico-Chemical Medicine, Malaja Pyrogovskaya 1-a, Moscow 119435, Russia. Electronic address: esmoline@mail.ru.
authors.forename: N V
authors.initials: NV
authors.lastname: Smolina
authors.affiliation: Research Institute of Physico-Chemical Medicine, Malaja Pyrogovskaya 1-a, Moscow 119435, Russia. Electronic address: lemik2007@yandex.ru.
authors.forename: E V
authors.initials: EV
authors.lastname: Mikhalchik
authors.affiliation: Lomonosov Moscow State University, Department of Chemistry, Leninskiye Gory 1-3, 119991 Moscow, Russia. Electronic address: nbalab2008@gmail.com.
authors.forename: N G
authors.initials: NG
authors.lastname: Balabushevich
authors.affiliation: Research Institute of Physico-Chemical Medicine, Malaja Pyrogovskaya 1-a, Moscow 119435, Russia. Electronic address: i.g.a@list.ru.
authors.forename: G A
authors.initials: GA
authors.lastname: Ibragimova
authors.affiliation: Institute of Nice Hair, Spartakovskaya sq 14 Build. 4, Office 4107, 1005005 Moscow, Russia. Electronic address: aida2010@mail.ru.
authors.forename: A G
authors.initials: AG
authors.lastname: Gadzhigoroeva
authors.affiliation: Institute of Nice Hair, Spartakovskaya sq 14 Build. 4, Office 4107, 1005005 Moscow, Russia. Electronic address: dmilena23@mail.ru.
authors.forename: E I
authors.initials: EI
authors.lastname: Dmitrieva
authors.affiliation: Research Institute of Physico-Chemical Medicine, Malaja Pyrogovskaya 1-a, Moscow 119435, Russia. Electronic address: gdobretsov@mail.ru.
authors.forename: G E
authors.initials: GE
authors.lastname: Dobretsov
mesh_terms: D006197:Hair / Q000737:chemistry* / Q000528:radiation effects*; D006801:Humans; D011506:Proteins / Q000737:chemistry*; D012995:Solubility; D013438:Sulfhydryl Compounds / Q000032:analysis; D014466:Ultraviolet Rays
chemical_list: D011506:Proteins; D013438:Sulfhydryl Compounds
keywords: Hair photodegradation; Soluble proteins; Sulfhydryl groups; Tryptophan fluorescence