title: Gene expression changes during Giardia-host cell interactions in serum-free medium.
abstract: Serial Analysis of Gene Expression (SAGE) was used to quantify transcriptional changes in Giardia intestinalis during its interaction with human intestinal epithelial cells (IECs, HT-29) in serum free M199 medium. Transcriptional changes were compared to those in trophozoites alone in M199 and in TYI-S-33 Giardia growth medium. In total, 90 genes were differentially expressed, mainly those involved in cellular redox homeostasis, metabolism and small molecule transport but also cysteine proteases and structural proteins of the giardin family. Only 29 genes changed their expression due to IEC interaction and the rest were due to M199 medium. Although our findings generated a small dataset, it was consistent with our earlier microarray studies performed under different interaction conditions. This study has confined the number of genes in Giardia to a small subset that specifically change their expression due to interaction with IECs.
authors.affiliation: Department of Cell and Molecular Biology, Uppsala University, Uppsala, Sweden.
authors.forename: Marcela
authors.initials: M
authors.lastname: Ferella
authors.affiliation: Department of Pathology, Division of Infectious Disease, University of California, San Diego, CA, USA.
authors.forename: Barbara J
authors.initials: BJ
authors.lastname: Davids
authors.affiliation: Department of Cell Biology, University of Georgia, Athens, GA, USA.
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Cipriano
authors.affiliation: Progenity, Inc., Ann Arbor, MI, USA.
authors.forename: Shanda R
authors.initials: SR
authors.lastname: Birkeland
authors.affiliation: Department of Cell and Molecular Biology, Uppsala University, Uppsala, Sweden.
authors.forename: Daniel
authors.initials: D
authors.lastname: Palm
authors.affiliation: Department of Pathology, Division of Infectious Disease, University of California, San Diego, CA, USA.
authors.forename: Frances D
authors.initials: FD
authors.lastname: Gillin
authors.affiliation: Andrew McArthur Consulting, Hamilton, Ontario, Canada L8S 3P6.
authors.forename: Andrew G
authors.initials: AG
authors.lastname: McArthur
authors.affiliation: Department of Cell and Molecular Biology, Uppsala University, Uppsala, Sweden. Electronic address: staffan.svard@icm.uu.se.
authors.forename: Staffan
authors.initials: S
authors.lastname: Svärd
mesh_terms: D002460:Cell Line; D016895:Culture Media, Serum-Free; D004847:Epithelial Cells / Q000378:metabolism* / Q000469:parasitology*; D015870:Gene Expression; D020869:Gene Expression Profiling; D005872:Giardia / Q000502:physiology*; D005873:Giardiasis / Q000235:genetics / Q000469:parasitology; D054884:Host-Pathogen Interactions / Q000235:genetics*; D006801:Humans; D007413:Intestinal Mucosa; D059467:Transcriptome
chemical_list: D016895:Culture Media, Serum-Free
keywords: Giardia intestinalis; Host–parasite interactions; Intestinal epithelial cells; SAGE