title: ID1201, the ethanolic extract of the fruit of Melia toosendan ameliorates impairments in spatial learning and reduces levels of amyloid beta in 5XFAD mice.
abstract: A previous study has demonstrated the anti-amyloidogenic effects of the ethanolic extract of Meliae Fructus (ID1201) using cell lines with stably expressed human Swedish mutant APP695 and β-secretase 1, and 5Xfamilial AD (FAD) mice carrying five mutations. Here, we investigated the effects of ID1201 on cognitive impairment in 5XFAD mice. Daily administration of ID1201 was commenced at 3 months of age and continued for 3 months. Mice were serially trained in cued/response and place/spatial training tasks in the Morris water maze. After this training, testing for strategy preference was conducted. Non-transgenic control mice with vehicle treatment, vehicle-treated 5XFAD, and ID1201-treated 5XFAD mice showed equivalent performance in cued/response training. However, as training progressed to the subsequent place/spatial learning, vehicle-treated control and ID1201-treated 5XFAD mice differed significantly from vehicle-treated 5XFAD mice in measures of spatial learning (search error and adaptive spatial learning strategy). In the strategy preference test that followed, control mice preferred a place/spatial strategy relative to vehicle-treated 5XFAD mice, but differences between ID1201-treated 5XFAD mice and vehicle-treated 5XFAD mice were not significant. Additionally, ID1201 treatment reduced hippocampal levels of insoluble Aβ42 and increased cortical levels of soluble amyloid precursor protein α. These results indicate that ID1201 may possess potential as a therapeutic agent for Alzheimer's disease by decreasing Aβ deposits.
authors.affiliation: Department of Biological Sciences, Konkuk University, Seoul 143-701, Republic of Korea.
authors.forename: Woo-Hyun
authors.initials: WH
authors.lastname: Cho
authors.affiliation: Department of Biological Sciences, Konkuk University, Seoul 143-701, Republic of Korea.
authors.forename: Jung-Cheol
authors.initials: JC
authors.lastname: Park
authors.affiliation: Department of Biological Sciences, Konkuk University, Seoul 143-701, Republic of Korea.
authors.forename: Dong-Hee
authors.initials: DH
authors.lastname: Kim
authors.affiliation: Department of Biological Sciences, Konkuk University, Seoul 143-701, Republic of Korea.
authors.forename: Min-Su
authors.initials: MS
authors.lastname: Kim
authors.affiliation: Department of Biological Sciences, Konkuk University, Seoul 143-701, Republic of Korea.
authors.forename: Sun-Young
authors.initials: SY
authors.lastname: Lee
authors.affiliation: ILDONG Research Laboratories, ILDONG Pharmaceutical Co. Ltd., Hwaseong, Gyeonggi 445-710, Republic of Korea.
authors.forename: Hanbyeol
authors.initials: H
authors.lastname: Park
authors.affiliation: ILDONG Research Laboratories, ILDONG Pharmaceutical Co. Ltd., Hwaseong, Gyeonggi 445-710, Republic of Korea.
authors.forename: Jae-Hoon
authors.initials: JH
authors.lastname: Kang
authors.affiliation: ILDONG Research Laboratories, ILDONG Pharmaceutical Co. Ltd., Hwaseong, Gyeonggi 445-710, Republic of Korea.
authors.forename: Seung-Woo
authors.initials: SW
authors.lastname: Yeon
authors.affiliation: Department of Biological Sciences, Konkuk University, Seoul 143-701, Republic of Korea. Electronic address: jshan06@konkuk.ac.kr.
authors.forename: Jung-Soo
authors.initials: JS
authors.lastname: Han
mesh_terms: D016229:Amyloid beta-Peptides / Q000378:metabolism*; D000818:Animals; D002540:Cerebral Cortex / Q000187:drug effects / Q000378:metabolism; D000431:Ethanol; D005260:Female; D005638:Fruit / Q000737:chemistry*; D008297:Male; D018782:Maze Learning / Q000187:drug effects; D031585:Melia / Q000737:chemistry*; D008817:Mice, Mutant Strains; D010936:Plant Extracts / Q000494:pharmacology*; D012997:Solvents; D065853:Spatial Learning / Q000187:drug effects*
chemical_list: D016229:Amyloid beta-Peptides; D010936:Plant Extracts; D012997:Solvents; D000431:Ethanol
keywords: 5XFAD; Amyloid beta; Amyloid precursor protein; ID1201; Spatial memory