title: Low-level laser therapy improves peri-implant bone formation: resonance frequency, electron microscopy, and stereology findings in a rabbit model.
abstract: Previous studies have reported positive effects of low-level laser therapy (LLLT) on bone healing. This study evaluated the effects of LLLT on peri-implant healing in vivo. Thirty-two rabbits had their mandibular left incisors removed, followed by immediate insertion of a dental implant into the fresh socket. Animals were assigned randomly to four groups: control (non-irradiated) or LLLT at three different doses per session: 5J/cm(2), 10J/cm(2), and 20J/cm(2). A GaAlAs laser (830nm, 50mW) was applied every 48h for 13 days, starting immediately after surgery. The implant stability quotient (ISQ) was measured using resonance frequency analysis upon implant insertion and immediately after death, 30 days after the last application. Tissues were prepared for scanning electron microscopy (SEM) and stereology. Variables measured were bone-implant contact (BIC) and bone neoformation within implant threads at three different sites. The results showed better ISQ for the 20J/cm(2) group (P=0.003). BIC values were significantly higher (P<0.05) in the 20J/cm(2) group, on both SEM and stereology. Bone area values were better in the 10J/cm(2) (P=0.036) and 20J/cm(2) (P=0.016) groups compared to the control group. Under these conditions, LLLT enhanced peri-implant bone repair, improving stability, BIC, and bone neoformation. The findings support and suggest parameters for the design of clinical trials using LLLT after implant placement.
authors.affiliation: School of Dentistry and Hospital de Clínicas de Porto Alegre, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil.
authors.forename: F V
authors.initials: FV
authors.lastname: Gomes
authors.affiliation: School of Dentistry, Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS), Porto Alegre, RS, Brazil.
authors.forename: L
authors.initials: L
authors.lastname: Mayer
authors.affiliation: School of Dentistry and Hospital de Clínicas de Porto Alegre, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil.
authors.forename: F P
authors.initials: FP
authors.lastname: Massotti
authors.affiliation: School of Dentistry and Hospital de Clínicas de Porto Alegre, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil. Electronic address: carlos.baraldi@ufrgs.br.
authors.forename: C E
authors.initials: CE
authors.lastname: Baraldi
authors.affiliation: School of Dentistry and Hospital de Clínicas de Porto Alegre, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil.
authors.forename: D
authors.initials: D
authors.lastname: Ponzoni
authors.affiliation: School of Dentistry, Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS), Porto Alegre, RS, Brazil.
authors.forename: J B B
authors.initials: JB
authors.lastname: Webber
authors.affiliation: Hospital Cristo Redentor, Grupo Hospitalar Conceição, Brazilian Ministry of Health, Porto Alegre, RS, Brazil.
authors.forename: M G
authors.initials: MG
authors.lastname: de Oliveira
mesh_terms: D000818:Animals; D003758:Dental Implantation, Endosseous / Q000379:methods*; D015921:Dental Implants; D028022:Low-Level Light Therapy / Q000379:methods*; D008297:Male; D008334:Mandible / Q000601:surgery; D008855:Microscopy, Electron, Scanning; D010012:Osteogenesis / Q000528:radiation effects*; D011817:Rabbits; D020390:Tooth Socket / Q000601:surgery; D014945:Wound Healing / Q000528:radiation effects*
chemical_list: D015921:Dental Implants
keywords: dental implants; histology; laser therapy; low-level; osseointegration; scanning electron microscopy