title: E-cadherin and CD10 expression in atypical hyperplastic and malignant endometrial lesions.
abstract: BACKGROUND
  Loss of E-cadherin is a critical step for development and progression of malignant tumors. CD10; a marker of non-neoplastic and neoplastic endometrial stroma, is associated with aggressiveness of many epithelial malignancies.
  
  
  OBJECTIVE
  To evaluate expression and correlation of E-cadherin and CD10 in endometrial lesions and their possible role in differentiating atypical endometrial hyperplasia from endometrial carcinoma. The association of E-cadherin and CD10 expression with clinico-pathological parameters of endometrial carcinoma was also investigated.
  
  
  METHODS
  Fifty four cases including 28 endometrial carcinomas; 19 endometrial hyperplasia and 7 cases of normal endometrial changes were enrolled for this study. The expression of E-cadherin and CD10 was evaluated by immunohistochemistry using the streptavidin-biotin technique.
  
  
  RESULTS
  There was a strong association between malignant change of endometrial glands and membrano-cytoplasmic localization of E-cadherin (p<0.001). Expression of E-cadherin but not CD10 was significantly higher in endometrial carcinomas compared to atypical endometrial hyperplasia (p<0.01). Expression of E-cadherin was not associated with CD10 expression in different endometrial lesions. High grade tumors expressed low levels of both E-cadherin (p<0.01) and CD10 (p<0.05) and serous endometrial carcinoma had low E-cadherin and CD10 expression compared to endometrioid carcinoma (p<0.01 and <0.05, respectively). Expression of both molecules showed no association with depth of tumor invasion or FIGO stage. Tumors with lower E-cadherin or CD10 expression had higher rates of vascular tumor emboli (p<0.01 and <0.07, respectively).
  
  
  CONCLUSIONS
  Although expression of E-cadherin and CD10 in endometrial lesions was not correlated, reduced expression of both molecules could be critical for progression of endometrial carcinoma.
authors.affiliation: Department of Pathology, Faculty of Medicine, Sohag University, Egypt. Electronic address: ahmed_ahmed4@med.sohag.edu.eg.
authors.forename: Ahmed R H
authors.initials: AR
authors.lastname: Ahmed
authors.affiliation: Department of Pathology, Faculty of Medicine, Sohag University, Egypt. Electronic address: eman_shalabi@hotmail.com.
authors.forename: Eman M S
authors.initials: EM
authors.lastname: Muhammad
mesh_terms: D000328:Adult; D000368:Aged; D015703:Antigens, CD; D014408:Biomarkers, Tumor; D001710:Biotin / Q000737:chemistry; D015820:Cadherins / Q000378:metabolism*; D002277:Carcinoma / Q000378:metabolism*; D002454:Cell Differentiation; D003593:Cytoplasm / Q000378:metabolism; D016889:Endometrial Neoplasms / Q000378:metabolism*; D004717:Endometrium / Q000378:metabolism*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D006965:Hyperplasia / Q000473:pathology*; D007150:Immunohistochemistry; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis; D015260:Neprilysin / Q000378:metabolism*; D019809:Streptavidin / Q000737:chemistry; D055815:Young Adult
chemical_list: D015703:Antigens, CD; D014408:Biomarkers, Tumor; C513384:CDH1 protein, human; D015820:Cadherins; D001710:Biotin; D019809:Streptavidin; D015260:Neprilysin
keywords: CD10; E-cadherin; Endometrial carcinoma; FIGO stage; Vascular tumor emboli