title: The Ras/Rap GTPase activating protein RASA3: from gene structure to in vivo functions.
abstract: RASA3 (or GTPase Activating Protein III, R-Ras GTPase-activating protein, GAP1(IP4BP)) is a GTPase activating protein of the GAP1 subfamily which targets Ras and Rap1. RASA3 was originally purified from pig platelet membranes through its intrinsic ability to bind inositol 1,3,4,5-tetrakisphosphate (I(1,3,4,5)P4) with high affinity, hence its first name GAP1(IP4BP) (for GAP1 subfamily member which binds I(1,3,4,5)P4). RASA3 was thus the first I(1,3,4,5)P4 receptor identified and cloned. The in vitro and in vivo functions of RASA3 remained somewhat elusive for a long time. However, recently, using genetically-modified mice and cells derived from these mice, the function of RASA3 during megakaryopoiesis, megakaryocyte adhesion and migration as well as integrin signaling has been reported. The goal of this review is thus to summarize and comment recent and less recent data in the literature on RASA3, in particular on the in vivo function of this specific GAP1 subfamily member.
authors.affiliation: Laboratoire de Génétique Fonctionnelle, GIGA-Signal Transduction, GIGA B34, Université de Liège, Avenue de l'Hôpital 1, B-4000 Liège, Belgium; Secteur de Biochimie Métabolique, Département des Sciences Fonctionnelles, Faculté de Médecine Vétérinaire, Université de Liège, Boulevard de Colonster 20, 4000 Liège, Belgium; Welbio, Belgium. Electronic address: sschurmans@ulg.ac.be.
authors.forename: Stéphane
authors.initials: S
authors.lastname: Schurmans
authors.affiliation: Institut de Recherches Interdisciplinaires en Biologie Humaine et Moléculaire (IRIBHM), Institut de Biologie et de Médecine Moléculaires (IBMM), Faculté de Médecine, Université Libre de Bruxelles, Rue des Professeurs Jeener et Brachet 12, 6041 Gosselies, Belgium. Electronic address: selenapolizzi@hotmail.com.
authors.forename: Séléna
authors.initials: S
authors.lastname: Polizzi
authors.affiliation: Laboratoire de Génétique Fonctionnelle, GIGA-Signal Transduction, GIGA B34, Université de Liège, Avenue de l'Hôpital 1, B-4000 Liège, Belgium; Welbio, Belgium.
authors.forename: Ariane
authors.initials: A
authors.lastname: Scoumanne
authors.affiliation: Laboratoire de Génétique Fonctionnelle, GIGA-Signal Transduction, GIGA B34, Université de Liège, Avenue de l'Hôpital 1, B-4000 Liège, Belgium.
authors.forename: Sufyan
authors.initials: S
authors.lastname: Sayyed
authors.affiliation: Laboratoire de Génétique Fonctionnelle, GIGA-Signal Transduction, GIGA B34, Université de Liège, Avenue de l'Hôpital 1, B-4000 Liège, Belgium; Welbio, Belgium.
authors.forename: Patricia
authors.initials: P
authors.lastname: Molina-Ortiz
mesh_terms: D000818:Animals; D002448:Cell Adhesion / Q000502:physiology; D002465:Cell Movement / Q000502:physiology*; D020690:GTPase-Activating Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D007295:Inositol Phosphates; D051379:Mice; D008822:Mice, Transgenic; D018160:Receptors, Cytoplasmic and Nuclear / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction / Q000502:physiology*; D034061:Thrombopoiesis / Q000502:physiology*
chemical_list: D020690:GTPase-Activating Proteins; D007295:Inositol Phosphates; C583390:RASA3 protein, mouse; D018160:Receptors, Cytoplasmic and Nuclear; C061107:inositol-1,3,4,5-tetrakisphosphate receptor; C081655:inositol-1,3,4,5-tetrakisphosphate
keywords: Cancer; GTPase activating protein; Leukemia; Megakaryocyte; Platelet; RASA3; Rap; Ras