title: Cross reactivity of Neisseria meningitidis crgA diagnostic PCR primers with nontypeable haemophilus influenzae.
abstract: BACKGROUND
  CrgA based PCR are usually used for diagnosis of Neisseria meningitidis while the crgA gene was observed in the genomes of Haemophilus influenzae. In this study, we aimed to evaluate whether the crgA primers routinely used in the diagnosis of N. meningitidis could cross react with H. influenzae isolates.
  
  
  METHODS
  A diagnostic test study analysis of sixty-two H. influenzae isolates from oropharyngeal swabs of healthy individuals aged 9 to 11 years between 2011 and 2012, using commonly used crgA primers for diagnostic analysis of N. meningitidis.
  
  
  RESULTS
  It revealed that 19.3% nontypable H. influenzae isolates were positive for the crgA gene. All the biotype IV H. influenzae isolates were crgA PCR positive.
  
  
  CONCLUSIONS
  Our study has shown a significant finding of crgA gene especially in bitotype IV nontypable H. influenzae by N. meningitidis crgA diagnostic PCR primers. It is necessary to further evaluate the prevalence of the crgA gene in more non-typable H. influenzae strains, particularly invasive strains.
authors.forename: Tiejun
authors.initials: T
authors.lastname: Hou
authors.forename: Quanli
authors.initials: Q
authors.lastname: Du
authors.forename: Lilei
authors.initials: L
authors.lastname: Wang
authors.forename: Haijian
authors.initials: H
authors.lastname: Zhou
authors.forename: Yanli
authors.initials: Y
authors.lastname: Xi
authors.forename: Zhijun
authors.initials: Z
authors.lastname: Chen
authors.forename: Zengguo
authors.initials: Z
authors.lastname: Wang
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics*; D001431:Bacteriological Techniques; D002648:Child; D017931:DNA Primers; D004269:DNA, Bacterial / Q000032:analysis*; D006193:Haemophilus influenzae / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D064368:Healthy Volunteers; D006801:Humans; D009345:Neisseria meningitidis / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D009960:Oropharynx / Q000382:microbiology; D016133:Polymerase Chain Reaction; D011237:Predictive Value of Tests; D015203:Reproducibility of Results; D014157:Transcription Factors / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; C406769:CrgA protein, Neisseria meningitidis; D017931:DNA Primers; D004269:DNA, Bacterial; D014157:Transcription Factors