title: Comparison of the biological effects of (18)F at different intracellular levels.
abstract: We herein examined the biological effects of cells treated with (18)F labeled drugs for positron emission tomography (PET). The relationship between the intracellular distribution of (18)F and levels of damaged DNA has yet to be clarified in detail. We used culture cells (Chinese Hamster Ovary cells) treated with two types of (18)F labeled drugs, fluorodeoxyglucose (FDG) and fluorine ion (HF). FDG efficiently accumulated in cells, whereas HF did not. To examine the induction of DNA double strand breaks (DSB), we measured the number of foci for 53BP1 that formed at the site of DNA DSB. The results revealed that although radioactivity levels were the same, the induction of 53BP1 foci was stronger in cells treated with (18)F-FDG than in those treated with (18)F-HF. The clonogenic survival of cells was significantly lower with (18)F-FDG than with (18)F-HF. We concluded that the efficient accumulation of (18)F in cells led to stronger biological effects due to more severe cellular lethality via the induction of DNA DSB.
authors.affiliation: Advanced Molecular Imaging Center, Faculty of Medicine, Oita University, 1-1 Idaigaoka, Hasama-machi, Yufu City, Oita 879-5593, Japan. Electronic address: kashino@oita-u.ac.jp.
authors.forename: Genro
authors.initials: G
authors.lastname: Kashino
authors.affiliation: Advanced Molecular Imaging Center, Faculty of Medicine, Oita University, 1-1 Idaigaoka, Hasama-machi, Yufu City, Oita 879-5593, Japan.
authors.forename: Kazutaka
authors.initials: K
authors.lastname: Hayashi
authors.affiliation: Advanced Molecular Imaging Center, Faculty of Medicine, Oita University, 1-1 Idaigaoka, Hasama-machi, Yufu City, Oita 879-5593, Japan.
authors.forename: Kazumasa
authors.initials: K
authors.lastname: Douhara
authors.affiliation: Department of Radiology, Faculty of Medicine, Oita University, 1-1 Idaigaoka, Hasama-machi, Yufu City, Oita 879-5593, Japan.
authors.forename: Shinko
authors.initials: S
authors.lastname: Kobashigawa
authors.affiliation: Department of Radiology, Faculty of Medicine, Oita University, 1-1 Idaigaoka, Hasama-machi, Yufu City, Oita 879-5593, Japan.
authors.forename: Hiromu
authors.initials: H
authors.lastname: Mori
mesh_terms: D000818:Animals; D016466:CHO Cells; D002470:Cell Survival / Q000528:radiation effects; D003114:Colony-Forming Units Assay; D006224:Cricetinae; D003412:Cricetulus; D053903:DNA Breaks, Double-Stranded; D004307:Dose-Response Relationship, Radiation; D005462:Fluorine Radioisotopes / Q000009:adverse effects* / Q000493:pharmacokinetics*; D019788:Fluorodeoxyglucose F18 / Q000009:adverse effects* / Q000493:pharmacokinetics*; D007424:Intracellular Fluid / Q000378:metabolism; D015162:Micronucleus Tests; D049268:Positron-Emission Tomography / Q000009:adverse effects*; D019275:Radiopharmaceuticals / Q000009:adverse effects* / Q000493:pharmacokinetics*
chemical_list: D005462:Fluorine Radioisotopes; D019275:Radiopharmaceuticals; D019788:Fluorodeoxyglucose F18
keywords: DNA DSB; FDG-PET; Positron; Radiobiology