title: Flow cytometry and solid organ transplantation: a perfect match.
abstract: In the field of transplantation, flow cytometry serves a well-established role in pre-transplant crossmatching and monitoring immune reconstitution following hematopoietic stem cell transplantation. The capabilities of flow cytometers have continuously expanded and this combined with more detailed knowledge of the constituents of the immune system, their function and interaction and newly developed reagents to study these parameters have led to additional utility of flow cytometry-based analyses, particularly in the post-transplant setting. This review discusses the impact of flow cytometry on managing alloantigen reactions, monitoring opportunistic infections and graft rejection and gauging immunosuppression in the context of solid organ transplantation.
authors.affiliation: Laboratory of Flow and Image Cytometry, Department of Pathology and Laboratory Medicine, Roswell Park Cancer Institute , Buffalo, New York , USA , and.
authors.forename: Orla
authors.initials: O
authors.lastname: Maguire
authors.forename: Joseph D
authors.initials: JD
authors.lastname: Tario
authors.forename: Thomas C
authors.initials: TC
authors.lastname: Shanahan
authors.forename: Paul K
authors.initials: PK
authors.lastname: Wallace
authors.forename: Hans
authors.initials: H
authors.lastname: Minderman
mesh_terms: D000818:Animals; D002469:Cell Separation; D005434:Flow Cytometry; D006084:Graft Rejection / Q000276:immunology* / Q000517:prevention & control; D018380:Hematopoietic Stem Cell Transplantation; D006648:Histocompatibility / Q000276:immunology; D006650:Histocompatibility Testing; D006801:Humans; D007108:Immune Tolerance; D007519:Isoantigens / Q000276:immunology*; D015166:Monitoring, Immunologic / Q000379:methods; D009894:Opportunistic Infections / Q000276:immunology*; D016377:Organ Transplantation
chemical_list: D007519:Isoantigens
keywords: Flow cytometry; immune monitoring; solid organ transplantation