title: Transcriptome analysis of Escherichia coli O157:H7 grown in vitro in the sterile-filtrated cecal content of human gut microbiota associated rats reveals an adaptive expression of metabolic and virulence genes.
abstract: In developed countries, enterohemorrhagic Escherichia coli (EHEC) O157:H7 is a leading cause of bloody diarrhea and renal failures in human. Understanding strategies employed by EHEC to colonize the intestine is of major importance since to date no cure exists to eradicate the pathogen. In this study, the adaptive response of EHEC to the intestinal milieu conditioned by a human microbiota was examined. A transcriptomic analysis was performed on the EHEC strain EDL933 incubated in vitro in the sterile-filtrated cecal content of human microbiota-associated rats (HMC) compared with EDL933 incubated in the sterile-filtrated cecal content of germ-free rat (GFC). EDL933 switches from a glycolytic metabolic profile in the GFC to an anaplerotic metabolic profile in HMC. The expression of several catabolism genes was strongly affected such as those involved in the utilization of sugars, glycerol, N-acetylneuraminic acid, amino acids and secondary metabolites. Interestingly, expression level of critical EHEC O157:H7 virulence genes including genes from the locus of enterocyte effacement was reduced in HMC. Altogether, these results contribute to the understanding of EHEC adaptive response to a digestive content and highlight the ability of the microbiota to repress EHEC virulence gene expression.
authors.affiliation: Centre de Recherche en Infectiologie Porcine et Aviaire (CRIPA), Université de Montréal, Faculté de médecine vétérinaire, Saint-Hyacinthe, QC, Canada.
authors.forename: Guillaume
authors.initials: G
authors.lastname: Le Bihan
authors.affiliation: INRA, UR454 Unité de Microbiologie, F-63122 Saint-Genès-Champanelle, France. Electronic address: gregory.jubelin@clermont.inra.fr.
authors.forename: Grégory
authors.initials: G
authors.lastname: Jubelin
authors.affiliation: Centre de Recherche en Infectiologie Porcine et Aviaire (CRIPA), Université de Montréal, Faculté de médecine vétérinaire, Saint-Hyacinthe, QC, Canada.
authors.forename: Philippe
authors.initials: P
authors.lastname: Garneau
authors.affiliation: INRA, UR454 Unité de Microbiologie, F-63122 Saint-Genès-Champanelle, France.
authors.forename: Annick
authors.initials: A
authors.lastname: Bernalier-Donadille
authors.affiliation: INRA, UR454 Unité de Microbiologie, F-63122 Saint-Genès-Champanelle, France.
authors.forename: Christine
authors.initials: C
authors.lastname: Martin
authors.affiliation: Groupe de Recherche en Pharmacologie Animal du Québec (GREPAQ), Département de biomédecine vétérinaire, Faculté de médecine vétérinaire, Université de Montréal, Saint-Hyacinthe, QC, Canada.
authors.forename: Francis
authors.initials: F
authors.lastname: Beaudry
authors.affiliation: Centre de Recherche en Infectiologie Porcine et Aviaire (CRIPA), Université de Montréal, Faculté de médecine vétérinaire, Saint-Hyacinthe, QC, Canada. Electronic address: josee.harel@umontreal.ca.
authors.forename: Josée
authors.initials: J
authors.lastname: Harel
mesh_terms: D000222:Adaptation, Physiological; D000328:Adult; D000818:Animals; D002432:Cecum / Q000382:microbiology*; D019453:Escherichia coli O157 / Q000235:genetics / Q000254:growth & development / Q000502:physiology*; D020869:Gene Expression Profiling; D006801:Humans; D008297:Male; D053858:Metabolic Networks and Pathways / Q000235:genetics; D056265:Microbial Interactions; D064307:Microbiota; D051381:Rats; D037521:Virulence Factors / Q000235:genetics
chemical_list: D037521:Virulence Factors
keywords: Enterohemorrhagic Escherichia coli; Intestinal milieu; Metabolism; Transcriptome; Virulence