title: Hyperoxygenation attenuated a murine model of atopic dermatitis through raising skin level of ROS.
abstract: Atopic dermatitis (AD) is a chronic inflammatory skin disease resulting from excessive stimulation of immune cells. Traditionally, reactive oxygen species (ROS) have been implicated in the progression of inflammatory diseases, but several opposing observations suggest the protective role of ROS in inflammatory disease. Recently, we demonstrated ROS prevented imiquimod-induced psoriatic dermatitis through enhancing regulatory T cell function. Thus, we hypothesized AD might also be attenuated in elevated levels of ROS through tissue hyperoxygenation, such as by hyperbaric oxygen therapy (HBOT) or applying an oxygen-carrying chemical, perfluorodecalin (PFD). Elevated levels of ROS in the skin have been demonstrated directly by staining with dihydroethidum as well as indirectly by immunohistochemistry (IHC) for indoleamine 2,3-dioxygenase (IDO). A murine model of AD was developed by repeated application of a chemical irritant (1% 2,4-dinitrochlorobenzene) and house dust mite (Dermatophagoide farinae) extract on one ear of BALB/c mice. The results showed treatment with HBOT or PFD significantly attenuated AD, comparably with 0.1% prednicarbate without any signs of side effects, such as telangiectasia. The expressions of interleukin-17A and interferon-γ were also decreased in the AD lesions by treatment with HBOT or PFD. Enhanced expression of IDO and reduced level of hypoxia-inducible factor-1α, in association with increased frequency of FoxP3+ regulatory T cells in the AD lesions, might be involved in the underlying mechanism of oxygen therapy. Taken together, it was suggested that tissue hyperoxygenation, by HBOT or treatment with PFD, might attenuate AD through enhancing skin ROS level.
authors.affiliation: Department of Microbiology, Ewha Womans University Graduate School of Medicine, Seoul, Republic of Korea.
authors.forename: Hyung-Ran
authors.initials: HR
authors.lastname: Kim
authors.affiliation: Academy of Immunology and Microbiology (AIM), Institute for Basic Science (IBS), Pohang, Republic of Korea; Division of Integrative Biosciences and Biotechnology (IBB), Pohang University of Science and Technology, Pohang, Republic of Korea.
authors.forename: Jung-Hwan
authors.initials: JH
authors.lastname: Kim
authors.affiliation: Department of Microbiology, Ewha Womans University Graduate School of Medicine, Seoul, Republic of Korea.
authors.forename: Eun-Jeong
authors.initials: EJ
authors.lastname: Choi
authors.affiliation: Ewha Womans University High School, Seoul, Republic of Korea.
authors.forename: Yeo Kyong
authors.initials: YK
authors.lastname: Lee
authors.affiliation: Pathology, National Health Insurance Cooperation Ilsan Hospital, Koyang, Republic of Korea.
authors.forename: Jeong-Hae
authors.initials: JH
authors.lastname: Kie
authors.affiliation: Academy of Immunology and Microbiology (AIM), Institute for Basic Science (IBS), Pohang, Republic of Korea; Division of Integrative Biosciences and Biotechnology (IBB), Pohang University of Science and Technology, Pohang, Republic of Korea.
authors.forename: Myoung Ho
authors.initials: MH
authors.lastname: Jang
authors.affiliation: Department of Microbiology, Ewha Womans University Graduate School of Medicine, Seoul, Republic of Korea.
authors.forename: Ju-Young
authors.initials: JY
authors.lastname: Seoh
mesh_terms: D000818:Animals; D003876:Dermatitis, Atopic / Q000139:chemically induced / Q000378:metabolism / Q000473:pathology* / Q000628:therapy*; D004137:Dinitrochlorobenzene; D004195:Disease Models, Animal; D005466:Fluorocarbons / Q000008:administration & dosage / Q000627:therapeutic use*; D006931:Hyperbaric Oxygenation; D050503:Indoleamine-Pyrrole 2,3,-Dioxygenase / Q000378:metabolism; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D010100:Oxygen / Q000008:administration & dosage / Q000627:therapeutic use*; D039981:Pyroglyphidae / Q000737:chemistry; D017382:Reactive Oxygen Species / Q000378:metabolism*; D012867:Skin / Q000187:drug effects / Q000378:metabolism / Q000473:pathology*
chemical_list: D004137:Dinitrochlorobenzene; D005466:Fluorocarbons; D050503:Indoleamine-Pyrrole 2,3,-Dioxygenase; D017382:Reactive Oxygen Species; C008805:perfluorodecalin; D010100:Oxygen