title: Crystallization and preliminary X-ray diffraction studies of the C-terminal domain of Chlamydia trachomatis CdsD.
abstract: The inner membrane ring of the bacterial type III secretion system (TTSS) is composed of two proteins. In Chlamydia trachomatis this ring is formed by CdsD (gene name CT_664) and CdsJ (gene name CTA_0609). CdsD consists of 829 amino acids. The last 400 amino acids at its C-terminal end relate it to the type III secretion system YscD/HrpQ protein family. The C-terminal domain, consisting of amino acids 558-771, of C. trachomatis CdsD was overexpressed in Escherichia coli and purified using immobilized metal-affinity chromatography (IMAC) and size-exclusion chromatography. The protein was crystallized using the vapour-diffusion method. A data set was collected to 2.26 Å resolution. The crystals have the symmetry of space group C2, with unit-cell parameters a = 106.60, b = 23.91, c = 118.65 Å, β = 104.95°. According to the data analysis there is expected to be one molecule in the asymmetric unit, with a Matthews coefficient of 3.0 Å(3) Da(-1).
authors.affiliation: Faculty of Biochemistry and Molecular Medicine, University of Oulu and Biocenter Oulu, PO Box 5400, 90014 Oulu, Finland.
authors.forename: Gitte
authors.initials: G
authors.lastname: Meriläinen
authors.affiliation: Faculty of Biochemistry and Molecular Medicine, University of Oulu and Biocenter Oulu, PO Box 5400, 90014 Oulu, Finland.
authors.forename: Rik K
authors.initials: RK
authors.lastname: Wierenga
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry*; D058947:Bacterial Secretion Systems; D002692:Chlamydia trachomatis; D002846:Chromatography, Affinity; D003460:Crystallization; D017434:Protein Structure, Tertiary; D014961:X-Ray Diffraction
chemical_list: D001426:Bacterial Proteins; D058947:Bacterial Secretion Systems
keywords: CdsD; Chlamydia; type III secretion system