title: MAP1S controls microtubule stability throughout the cell cycle in human cells.
abstract: Summary Understanding the molecular basis for proper cell division requires a detailed functional analysis of microtubule (MT)-associated proteins. MT-associated protein 1S (MAP1S), the most ubiquitously expressed MAP1 family member, is required for accurate cell division. Here, using quantitative analysis of MT plus-end tracking, we show that MAP1S knockdown alters MT dynamics throughout the cell cycle. Surprisingly, MAP1S downregulation results in faster growing, yet short-lived, MTs in all cell cycle stages and in a global loss of MT acetylation. These aberrations correlate with severe defects in the final stages of cell division. In monopolar cytokinesis assays, we demonstrate that MAP1S guides MT-dependent initiation of cytokinesis. Our data underline the key role of MAP1S as a global regulator of MT stability and demonstrate a new primary function of MAP1S to regulate MT dynamics at the onset of cytokinesis.
authors.affiliation: Zentrum für Molekulare Biologie der Universität Heidelberg (ZMBH), DKFZ-ZMBH Alliance, Im Neuenheimer Feld 282, 69120 Heidelberg, Germany.
authors.forename: Justus
authors.initials: J
authors.lastname: Tegha-Dunghu
authors.affiliation: Zentrum für Molekulare Biologie der Universität Heidelberg (ZMBH), DKFZ-ZMBH Alliance, Im Neuenheimer Feld 282, 69120 Heidelberg, Germany.
authors.forename: Elena
authors.initials: E
authors.lastname: Bausch
authors.affiliation: European Molecular Biology Laboratory (EMBL), Advanced Light Microscopy Facility Programme, Meyerhostr.1, 69117 Heidelberg, Germany.
authors.forename: Beate
authors.initials: B
authors.lastname: Neumann
authors.affiliation: European Molecular Biology Laboratory (EMBL), Cell Biology and Biophysics Programme, Meyerhostr.1, 69117 Heidelberg, Germany.
authors.forename: Annelie
authors.initials: A
authors.lastname: Wuensche
authors.affiliation: European Molecular Biology Laboratory (EMBL), Cell Biology and Biophysics Programme, Meyerhostr.1, 69117 Heidelberg, Germany.
authors.forename: Thomas
authors.initials: T
authors.lastname: Walter
authors.affiliation: European Molecular Biology Laboratory (EMBL), Cell Biology and Biophysics Programme, Meyerhostr.1, 69117 Heidelberg, Germany.
authors.forename: Jan
authors.initials: J
authors.lastname: Ellenberg
authors.affiliation: Zentrum für Molekulare Biologie der Universität Heidelberg (ZMBH), DKFZ-ZMBH Alliance, Im Neuenheimer Feld 282, 69120 Heidelberg, Germany o.gruss@zmbh.uni-heidelberg.de.
authors.forename: Oliver J
authors.initials: OJ
authors.lastname: Gruss
mesh_terms: D000107:Acetylation; D002453:Cell Cycle; D048749:Cytokinesis; D055785:Gene Knockdown Techniques; D006367:HeLa Cells; D006801:Humans; D008856:Microscopy, Fluorescence; D018715:Microscopy, Video; D008869:Microtubule-Associated Proteins / Q000235:genetics / Q000378:metabolism*; D008870:Microtubules / Q000378:metabolism*; D011499:Protein Processing, Post-Translational; D034622:RNA Interference; D013997:Time Factors; D059008:Time-Lapse Imaging; D014162:Transfection
chemical_list: C493240:MAP1S protein, human; D008869:Microtubule-Associated Proteins
keywords: Cytokinesis; MAP1S; Microtubule; Microtubule dynamics; Microtubule-associated protein