title: Effect of thioredoxin peroxidase-1 gene disruption on the liver stages of the rodent malaria parasite Plasmodium berghei.
abstract: Phenotypic observation of thioredoxin peroxidase-1 (TPx-1) gene-disrupted Plasmodium berghei (TPx-1 KO) in the liver-stage was performed with an in vitro infection system in order to investigate defective liver-stage development in a mouse infection model. Indirect immunofluorescence microscopy assay with anti-circumsporozoite protein antibody revealed that in the liver schizont stage, TPx-1 KO parasite cells were significantly smaller than cells of the wild-type parent strain (WT). Indirect immunofluorescence microscopy assay with anti-merozoite surface protein-1 antibody, which was used to evaluate late schizont-stage development, indicated that TPx-1 KO schizont development was similar to WT strain development towards the merozoite-forming stage (mature schizont). However, fewer merozoites were produced in the mature TPx-1 KO schizont than in the mature WT schizont. Taken together, the results suggest that TPx-1 may be involved in merozoite formation during liver schizont development.
authors.affiliation: National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, 2-13 Inada-cho, Obihiro, Hokkaido 080-8555, Japan. Electronic address: kasiopeia0716@gmail.com.
authors.forename: Miho
authors.initials: M
authors.lastname: Usui
authors.affiliation: National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, 2-13 Inada-cho, Obihiro, Hokkaido 080-8555, Japan. Electronic address: shirono@obihiro.ac.jp.
authors.forename: Hirono
authors.initials: H
authors.lastname: Masuda-Suganuma
authors.affiliation: National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, 2-13 Inada-cho, Obihiro, Hokkaido 080-8555, Japan. Electronic address: fukumoto@obihiro.ac.jp.
authors.forename: Shinya
authors.initials: S
authors.lastname: Fukumoto
authors.affiliation: National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, 2-13 Inada-cho, Obihiro, Hokkaido 080-8555, Japan. Electronic address: amoj28@gmail.com.
authors.forename: Jose Ma M
authors.initials: JM
authors.lastname: Angeles
authors.affiliation: National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, 2-13 Inada-cho, Obihiro, Hokkaido 080-8555, Japan. Electronic address: hassanhakimi@gmail.com.
authors.forename: Hassan
authors.initials: H
authors.lastname: Hakimi
authors.affiliation: National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, 2-13 Inada-cho, Obihiro, Hokkaido 080-8555, Japan. Electronic address: ircpmi@obihiro.ac.jp.
authors.forename: Noboru
authors.initials: N
authors.lastname: Inoue
authors.affiliation: National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, 2-13 Inada-cho, Obihiro, Hokkaido 080-8555, Japan. Electronic address: skawazu@obihiro.ac.jp.
authors.forename: Shin-Ichiro
authors.initials: S
authors.lastname: Kawazu
mesh_terms: D000818:Animals; D055785:Gene Knockdown Techniques; D056945:Hep G2 Cells; D006801:Humans; D008099:Liver / Q000469:parasitology*; D020066:Merozoite Surface Protein 1 / Q000276:immunology; D052940:Merozoites / Q000254:growth & development; D051379:Mice; D054464:Peroxiredoxins / Q000235:genetics* / Q000378:metabolism*; D010641:Phenotype; D010962:Plasmodium berghei / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development*; D012377:Rodentia; D052939:Schizonts / Q000166:cytology / Q000254:growth & development / Q000378:metabolism
chemical_list: D020066:Merozoite Surface Protein 1; D054464:Peroxiredoxins
keywords: Liver stage; Malaria; Peroxiredoxin; Plasmodium berghei; Thioredoxin peroxidase