title: Comparative binding affinities of flavonoid phytochemicals with bovine serum albumin.
abstract: Dietary flavonoids show beneficial effects in the prevention of chronic diseases. However, flavonoid bioavailability is poor, probably due to their interaction with serum albumins. In the current work, the binding interactions of eight related flavonoids, sharing a similar core structure, with bovine serum albumin (BSA) were investigated by fluorescence spectroscopy. The binding affinities of the flavonoids with BSA were in the order hesperetin (KA=5.59 × 10(5))> quercetin (4.94 × 10(5)) > naringenin (3.04 × 10(5)) > isoquercitrin (4.66 × 10(4)) > icariin (3.60 × 10(4)) > rutin (1.65 × 10(4)) > hesperidin (2.50 × 10(3)) > naringin (8.70 × 10(2)). The associations of specific structural components of the flavonoids with their binding properties to BSA were also explored and hydrophobicity, functional group substituents, steric hindrance effects and the spatial arrangements of substituents seem to be the key factors for the affinities of flavonoids towards BSA. The results from the current work contribute to a better understanding of the transport of flavonoids in plasma and helping predict their physiological functions based on their intrinsic structures.
authors.affiliation: Department of Biochemistry, Dalian Medical University, Dalian,116044, China.
authors.forename: Shuqing
authors.initials: S
authors.lastname: Liu
authors.affiliation: Department of Biotechnology, Dalian Medical University, Dalian,116044, China.
authors.forename: Chunmei
authors.initials: C
authors.lastname: Guo
authors.affiliation: Department of Biochemistry, Dalian Medical University, Dalian,116044, China.
authors.forename: Yimeng
authors.initials: Y
authors.lastname: Guo
authors.affiliation: School of Bioengineering, Dalian Polytechnic University, Dalian 116034, China.
authors.forename: Hongshan
authors.initials: H
authors.lastname: Yu
authors.affiliation: Carlson School of Chemistry and Biochemistry, Clark University, Worcester, MA 01610, USA.
authors.forename: Frederick
authors.initials: F
authors.lastname: Greenaway
authors.affiliation: Department of Biotechnology, Dalian Medical University, Dalian,116044, China.
authors.forename: Ming-Zhong
authors.initials: MZ
authors.lastname: Sun
keywords: Binding capacity; Bovine serum albumin; Flavonoids; Function; Structure