title: Enterovirus 71 infects brain-derived neural progenitor cells.
abstract: Neural progenitor cells (NPCs) are stem cells that can differentiate into various neural lineage cells. The damage and loss of NPCs are associated with neurological conditions such as cognitive deficits and memory impairment. In a long-term study of patients with EV71, cognitive disorders were observed. Therefore, we hypothesized that NPCs may be permissive to EV71 infection. We demonstrated that NPCs are prone to EV71 infection and that these stem cells can support the active replication of this virus. Furthermore, EV71 infection triggers apoptosis, resulting in significant cell death in infected NPCs. However, EV71 did not replicate in the differentiated cell types that were tested. Our findings suggest that EV71 can infect NPCs and cause the depletion of these cells.
authors.affiliation: Department of Medical Biotechnology and Laboratory Science, Research Center for Emerging Viral Infections, Chang Gung University, 259 Wen-Hwa 1st Road, Kwei-Shan, Tao-Yuan, Taiwan, ROC; Department of Medical Biotechnology and Laboratory Science, College of Medicine, Chang Gung University, Kwei-Shan, Tao-Yuan, Taiwan, ROC. Electronic address: hihuang@mail.cgu.edu.tw.
authors.forename: Hsing-I
authors.initials: HI
authors.lastname: Huang
authors.affiliation: Department of Medical Biotechnology and Laboratory Science, Research Center for Emerging Viral Infections, Chang Gung University, 259 Wen-Hwa 1st Road, Kwei-Shan, Tao-Yuan, Taiwan, ROC; Graduate institute of Biomedical Sciences, Chang Gung University, Kwei-Shan, Tao-Yuan, Taiwan, ROC.
authors.forename: Jhao-Yin
authors.initials: JY
authors.lastname: Lin
authors.affiliation: Department of Medical Biotechnology and Laboratory Science, Research Center for Emerging Viral Infections, Chang Gung University, 259 Wen-Hwa 1st Road, Kwei-Shan, Tao-Yuan, Taiwan, ROC; Department of Medical Biotechnology and Laboratory Science, College of Medicine, Chang Gung University, Kwei-Shan, Tao-Yuan, Taiwan, ROC.
authors.forename: Hsin-Hsu
authors.initials: HH
authors.lastname: Chen
authors.affiliation: Department of Medical Biotechnology and Laboratory Science, College of Medicine, Chang Gung University, Kwei-Shan, Tao-Yuan, Taiwan, ROC.
authors.forename: Shiou-Bang
authors.initials: SB
authors.lastname: Yeh
authors.affiliation: Department of Medical Biotechnology and Laboratory Science, Research Center for Emerging Viral Infections, Chang Gung University, 259 Wen-Hwa 1st Road, Kwei-Shan, Tao-Yuan, Taiwan, ROC; Department of Medical Biotechnology and Laboratory Science, College of Medicine, Chang Gung University, Kwei-Shan, Tao-Yuan, Taiwan, ROC.
authors.forename: Rei-Lin
authors.initials: RL
authors.lastname: Kuo
authors.affiliation: Department of Medical Biotechnology and Laboratory Science, Research Center for Emerging Viral Infections, Chang Gung University, 259 Wen-Hwa 1st Road, Kwei-Shan, Tao-Yuan, Taiwan, ROC.
authors.forename: Kuo-Feng
authors.initials: KF
authors.lastname: Weng
authors.affiliation: Department of Medical Biotechnology and Laboratory Science, Research Center for Emerging Viral Infections, Chang Gung University, 259 Wen-Hwa 1st Road, Kwei-Shan, Tao-Yuan, Taiwan, ROC; Department of Medical Biotechnology and Laboratory Science, College of Medicine, Chang Gung University, Kwei-Shan, Tao-Yuan, Taiwan, ROC; Clinical Virology Lab, Department of Clinical Pathology, Chang Gung Memorial Hospital, Kwei-Shan, Tao-Yuan, Taiwan, ROC.
authors.forename: Shin-Ru
authors.initials: SR
authors.lastname: Shih
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000166:cytology / Q000502:physiology; D002454:Cell Differentiation; D029821:Enterovirus A, Human / Q000502:physiology*; D004769:Enterovirus Infections / Q000473:pathology / Q000821:virology*; D051379:Mice; D008813:Mice, Inbred ICR; D058953:Neural Stem Cells / Q000821:virology*; D009474:Neurons / Q000166:cytology / Q000502:physiology; D010948:Viral Plaque Assay
keywords: EV71; Neural progenitor cells; Permissiveness