title: Magnetic microparticles post-synthetically coated by hyaluronic acid as an enhanced carrier for microfluidic bioanalysis.
abstract: Iron oxide based particles functionalized by bioactive molecules have been utilized extensively in biotechnology and biomedicine. Despite their already proven advantages, instability under changing reaction conditions, non-specific sorption of biomolecules on the particles' surfaces, and iron oxide leakage from the naked particles can greatly limit their application. As confirmed many times, surface treatment with an appropriate stabilizer helps to minimize these disadvantages. In this work, we describe enhanced post-synthetic surface modification of superparamagnetic microparticles varying in materials and size using hyaluronic acid (HA) in various chain lengths. Scanning electron microscopy, atomic force microscopy, phase analysis light scattering and laser diffraction are the methods used for characterization of HA-coated particles. The zeta potential and thickness of HA-layer of HA-coated Dynabeads M270 Amine were -50 mV and 85 nm, respectively, and of HA-coated p(GMA-MOEAA)-NH2 were -38 mV and 140 nm, respectively. The electrochemical analysis confirmed the zero leakage of magnetic material and no reactivity of particles with hydrogen peroxide. The rate of non-specific sorption of bovine serum albumin was reduced up to 50% of the naked ones. The coating efficiency and suitability of biopolymer-based microparticles for magnetically active microfluidic devices were confirmed.
authors.affiliation: Department of Biological and Biochemical Sciences, Faculty of Chemical Technology, University of Pardubice, Studentska 573, 53210 Pardubice, Czech Republic; Department of Analytical Chemistry, Faculty of Chemical Technology, University of Pardubice, Studentska 573, 53210 Pardubice, Czech Republic.
authors.forename: Lucie
authors.initials: L
authors.lastname: Holubova
authors.affiliation: Joint Laboratory of Solid State Chemistry, Faculty of Chemical Technology, University of Pardubice, Studentska 573, 53210 Pardubice, Czech Republic.
authors.forename: Petr
authors.initials: P
authors.lastname: Knotek
authors.affiliation: Institute of Environmental and Chemical Engineering, Faculty of Chemical Technology, University of Pardubice, Studentska 573, 53210 Pardubice, Czech Republic.
authors.forename: Jiri
authors.initials: J
authors.lastname: Palarcik
authors.affiliation: Department of Biological and Biochemical Sciences, Faculty of Chemical Technology, University of Pardubice, Studentska 573, 53210 Pardubice, Czech Republic; Department of Analytical Chemistry, Faculty of Chemical Technology, University of Pardubice, Studentska 573, 53210 Pardubice, Czech Republic.
authors.forename: Michaela
authors.initials: M
authors.lastname: Cadkova
authors.affiliation: Department of Inorganic Technology, Faculty of Chemical Technology, University of Pardubice, Doubravice 41, 53210 Pardubice, Czech Republic.
authors.forename: Petr
authors.initials: P
authors.lastname: Belina
authors.affiliation: Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, Heyrovskeho sq. 2, 16206 Prague, Czech Republic.
authors.forename: Milan
authors.initials: M
authors.lastname: Vlcek
authors.affiliation: Department of Biological and Biochemical Sciences, Faculty of Chemical Technology, University of Pardubice, Studentska 573, 53210 Pardubice, Czech Republic.
authors.forename: Lucie
authors.initials: L
authors.lastname: Korecka
authors.affiliation: Department of Biological and Biochemical Sciences, Faculty of Chemical Technology, University of Pardubice, Studentska 573, 53210 Pardubice, Czech Republic. Electronic address: Zuzana.Bilkova@upce.cz.
authors.forename: Zuzana
authors.initials: Z
authors.lastname: Bilkova
mesh_terms: D055598:Chemical Phenomena; D005290:Ferric Compounds / Q000737:chemistry; D006820:Hyaluronic Acid / Q000737:chemistry*; D008280:Magnetics; D044085:Microfluidics / Q000379:methods*; D008855:Microscopy, Electron, Scanning; D010316:Particle Size; D012710:Serum Albumin, Bovine; D013499:Surface Properties
chemical_list: D005290:Ferric Compounds; C000499:ferric oxide; D012710:Serum Albumin, Bovine; D006820:Hyaluronic Acid
keywords: Coating; Hyaluronan; Hyaluronic acid; Magnetic microparticle; Microfluidic device; Particle characterization