title: Role of Fe-responsive genes in bioreduction and transport of ionic gold to roots of Arabidopsis thaliana during synthesis of gold nanoparticles.
abstract: Several studies have shown potassium chloroaurate (KAuCl4)-mediated synthesis of gold nanoparticles (AuNPs) by using extracts of different parts of diverse plant species. However, the mechanism underlying the formation of AuNPs in planta has far from being elucidated. Here, we report the molecular evidence towards the role of genes involved in iron (Fe) homeostasis during in planta synthesis of AuNPs in roots of Arabidopsis thaliana. Firstly, we examined the dosage-dependent effects of KAuCl4 treatment on primary root length (PRL), and meristematic activity of roots in transgenic CycB1;1::uidA. Compared to control seedling (0 ppm KAuCl4), PRL and meristematic activity of primary and lateral roots showed progressive attenuation in seedlings treated with higher concentrations of KAuCl4 (25 ppm or above). Therefore, subsequent studies on in planta synthesis of AuNPs, and molecular responses were carried out in roots of the seedlings treated with 10 ppm KAuCl4 for 7 d. TEM of KAuCl4-treated seedlings showed the presence of monodisperse AuNPs of different shapes and sizes in root biomatrix. There was a significant induction of FRO2 in KAuCl4-treated roots, and therefore its likely involvement in bioreduction of Au(3)(+) could be assumed. Elevated expression levels of Fe transporters IRT1 and IRT2 further suggested their potential role in transport of bioreduced Au(3+) across root membrane. Expression levels of other genes involved in Fe homeostasis, and also different members of zinc (Zn), phosphate (Pi), and potassium (K) transporter families remained unaffected by KAuCl4 treatment. An increased Au content in Fe-deprived roots further provided evidence towards the specific role of a subset of Fe-responsive genes during in planta synthesis of AuNPs.
authors.affiliation: Department of Biology, Western Kentucky University, Bowling Green, KY 42101-1080, USA; National Research Centre on Plant Biotechnology, Lal Bahadur Shastri Building, Pusa Campus, New Delhi 110012, India.
authors.forename: Ajay
authors.initials: A
authors.lastname: Jain
authors.affiliation: Department of Biology, Western Kentucky University, Bowling Green, KY 42101-1080, USA.
authors.forename: Bhaskaran
authors.initials: B
authors.lastname: Sinilal
authors.affiliation: Department of Biology, Western Kentucky University, Bowling Green, KY 42101-1080, USA.
authors.forename: Daniel L
authors.initials: DL
authors.lastname: Starnes
authors.affiliation: National Research Centre on Plant Biotechnology, Lal Bahadur Shastri Building, Pusa Campus, New Delhi 110012, India.
authors.forename: Raghavendrarao
authors.initials: R
authors.lastname: Sanagala
authors.affiliation: Department of Biology, Western Kentucky University, Bowling Green, KY 42101-1080, USA.
authors.forename: Sneha
authors.initials: S
authors.lastname: Krishnamurthy
authors.affiliation: Department of Biology, Western Kentucky University, Bowling Green, KY 42101-1080, USA. Electronic address: shiv.sahi@wku.edu.
authors.forename: Shivendra V
authors.initials: SV
authors.lastname: Sahi
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant; D006046:Gold; D053768:Metal Nanoparticles; D018517:Plant Roots / Q000235:genetics / Q000378:metabolism*
chemical_list: D029681:Arabidopsis Proteins; D006046:Gold
keywords: Arabidopsis thaliana; AuNPs; CycB1;1::uidA; Fe-responsive genes; In planta synthesis; Root biomatrix; Root growth