title: Involvement of CSE/ H2S in high glucose induced aberrant secretion of adipokines in 3T3-L1 adipocytes.
abstract: BACKGROUND
  Deregulated secretion of adipokines contributes to subclinical systemic inflammation associated with type 2 diabetes mellitus (T2DM). However, the mechanisms underlying are not fully understood. Hydrogen sulfide (H2S), as an endogenous gasotransmitter, possesses an anti-inflammation activity. The aim of this study was to examine the possible involvement of H2S in high glucose induced adipokine secretion in 3T3-L1 adipocytes.
  
  
  METHODS
  The expression of cystathionine-gamma-lyase (CSE), the H2S-forming enzyme, was evaluated by Western-blotting and real-time PCR. The secretion of TNF-α, MCP-1 and adiponectin was determined by radioimmunoassay and enzyme-linked immunosorbent assay (ELISA), respectively. Lentiviral empty vector and vector expressing mouse CSE were used for in vitro transduction.
  
  
  RESULTS
  High glucose (HG) significantly decreased CSE expression at both protein and mRNA levels in mature 3T3-L1 adipocytes. In parallel, HG significantly increased secretion of MCP-1 while decreasing secretion of adiponectin, but had no effect on secretion of TNF-α. HG induced changes in MCP-1 and adiponectin secretion were partly attenuated by forced expression of CSE or sodium hydrosulfide (NaHS), a source of exogenous H2S.
  
  
  CONCLUSIONS
  High glucose induces aberrant secretion of adipokines in mature 3T3-L1 adipocytes, favoring inflammation. The mechanism is partly related to inhibition of CSE/ H2S system.
authors.forename: Zhe
authors.initials: Z
authors.lastname: Pan
authors.forename: Hanbo
authors.initials: H
authors.lastname: Wang
authors.forename: Yuantao
authors.initials: Y
authors.lastname: Liu
authors.forename: Chunxiao
authors.initials: C
authors.lastname: Yu
authors.forename: Yuchao
authors.initials: Y
authors.lastname: Zhang
authors.forename: Jicui
authors.initials: J
authors.lastname: Chen
authors.forename: Xiangdong
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Endocrinology, Shandong Provincial Hospital affiliated to Shandong University; Shandong Clinical Medical Center of Endocrinology and Metabolism; Institute of Endocrinology and Metabolism; Shandong Academy of Clinical Medicine, 324 Jing 5 Rd, Jinan, Shandong 250021, P, R, China. guanqingbo163@163.com.
authors.forename: Qingbo
authors.initials: Q
authors.lastname: Guan
mesh_terms: D041721:3T3-L1 Cells; D050156:Adipogenesis; D054392:Adipokines / Q000378:metabolism*; D000818:Animals; D003542:Cystathionine gamma-Lyase / Q000235:genetics / Q000378:metabolism*; D015536:Down-Regulation; D004794:Enzyme Repression; D005947:Glucose / Q000502:physiology*; D006862:Hydrogen Sulfide / Q000378:metabolism*; D051379:Mice; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
chemical_list: D054392:Adipokines; D012333:RNA, Messenger; D003542:Cystathionine gamma-Lyase; D005947:Glucose; D006862:Hydrogen Sulfide