title: Effect of TRAIL in combination with DDP on the expression of MDR1 gene in gastric cancer cells.
abstract: BACKGROUND
  Gastric cancer is one of the most common malignant tumor, and gastric cancer is the second most common cause of cancer mortality worldwide. Although chemotherapy is one of the most important treatment options for gastric cancer, and could improve the overall survival rate and quality of live, one significant reason for its failure is multidrug resistance (MDR).
  
  
  OBJECTIVE
  To study the effect of tumour necrosis factor-related apoptosis-inducing ligand (TRAIL) combined with chemotherapeutic drug cisplatin (DDP) on the expression of multidrug resistance gene 1 (MDR1) in the gastric cancer cell line SGC-7901/VCR.
  
  
  METHODS
  SGC-7901/VCR cells were cultured with DDP and TRAIL in various concentrations. The apoptosis rate was separately measured by a flow cytometer in DDP (sub-toxic dose) alone, TRAIL (200 µg/l) alone and in a combination of the two. Expression levels of MDR1 mRNA and P-glycoprotein (P-gp) were detected by RT-PCR and ELISA analysis, respectively.
  
  
  RESULTS
  The apoptosis rate in the combination group was significantly higher than that in the other groups (p < 0.05). According to the results of RT-PCR and ELISA, the expressions of MDR1 mRNA and P-gp in the combination group were statistically significant different compared with other groups (p < 0.05).
  
  
  CONCLUSIONS
  The combination of TRAIL with DDP could reverse MDR phenotype in gastric cancer cell line SGC7901/VCR. The mechanism may be involved in the down-regulation of MDR1 mRNA and P-gp, which may play an essential role in overcoming the chemotherapeutic resistance of gastric cancer cells. This study indicates that a combination of chemotherapy and TRAIL may be an effective strategy to treat MDR gastric cancer.
authors.affiliation: Department of Gastroenterology, The Affiliated Provincial Hospital of Anhui Medical University, Hefei City, China.
authors.forename: Yu-Fang
authors.initials: YF
authors.lastname: Cui
authors.affiliation: Department of Gastroenterology, The Affiliated Provincial Hospital of Anhui Medical University, Hefei City, China.
authors.forename: Long-Shan
authors.initials: LS
authors.lastname: Yu
authors.affiliation: Department of Gastroenterology, The Affiliated Provincial Hospital of Anhui Medical University, Hefei City, China.
authors.forename: Hui-Qun
authors.initials: HQ
authors.lastname: Wang
authors.affiliation: Department of Gastroenterology, The Affiliated Provincial Hospital of Anhui Medical University, Hefei City, China.
authors.forename: Ya-Wen
authors.initials: YW
authors.lastname: Gou
authors.affiliation: Department of Gastroenterology, The Affiliated Provincial Hospital of Anhui Medical University, Hefei City, China.
authors.forename: Qiao-Ming
authors.initials: QM
authors.lastname: Wang
authors.affiliation: Department of Gastroenterology, The Affiliated Provincial Hospital of Anhui Medical University, Hefei City, China.
authors.forename: Kai-Guang
authors.initials: KG
authors.lastname: Zhang
keywords: MDR1; P-glycoprotein; TRAIL; gastric carcinoma