title: Renilla luciferase reporter assay to study 3'UTR-driven posttranscriptional regulations of OPRM1.
abstract: The regulation of MOR expression at the level of mRNA is relevant for its role in pain transmission and in other functions involving opioid receptors. Recently, the role of the 3'UTR in the posttranscriptional regulation of MOR expression has been highlighted. Here we describe a Renilla luciferase reporter assay for the study of the effect of any selective treatment on the 3'UTR-dependent regulation of OPRM1 in a model of glial cells.
authors.affiliation: Department of Pharmacy and Biotechnology (FaBiT), University of Bologna, Via Irnerio 48, 40126, Bologna, (BO), Italy.
authors.forename: Gabriele
authors.initials: G
authors.lastname: Vincelli
authors.forename: Andrea
authors.initials: A
authors.lastname: Bedini
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics*; D017930:Genes, Reporter; D008156:Luciferases / Q000235:genetics / Q000378:metabolism; D009457:Neuroglia / Q000378:metabolism; D011499:Protein Processing, Post-Translational / Q000235:genetics*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D017450:Receptors, Opioid, mu / Q000096:biosynthesis / Q000235:genetics*
chemical_list: D020413:3' Untranslated Regions; C485764:OPRM1 protein, human; D012333:RNA, Messenger; D017450:Receptors, Opioid, mu; D008156:Luciferases