title: Self-organized nanoparticles based on chitosan-folic acid and dextran succinate-doxorubicin conjugates for drug targeting.
abstract: Folic acid-decorated self-organized nanoparticles were fabricated to target folate receptor of cancer cells. Doxorubicin (DOX) was conjugated with carboxyl group of dextran backbone using succinic anhydride (DexSU-DOX). DOX-loaded self-organized nanoparticles were prepared by complexation with folic acid-grafted chitosan (ChitoFA) and DexSU-DOX. Nanoparticles in the aqueous environment have spherical shapes with average size less than 100 nm and their sizes were increased by coated with ChitoPEG or ChitoFA. At cell culture study with KB cells, ChitoFA coated nanoparticles (FADex NP) revealed folate-receptor mediated endocytosis to cancer cells and cell viability was significantly changed by folate receptor targeting. Tumor xenograft model of KB cells also showed similar results, i.e. FAdex NP efficiently inhibited growth of tumor compared to the treatment group with blocking of folate receptor. These results indicated that DOX-loaded nanoparticles of FADex NP are promising vehicle for anticancer drug targeting.
authors.affiliation: Department of Oriental Medicine Materials, Dongsin University, Naju, 520-714, Republic of Korea.
authors.forename: Kyung Dong
authors.initials: KD
authors.lastname: Lee
authors.forename: Seon-Hee
authors.initials: SH
authors.lastname: Choi
authors.forename: Da Hye
authors.initials: DH
authors.lastname: Kim
authors.forename: Hye-Young
authors.initials: HY
authors.lastname: Lee
authors.forename: Ki-Choon
authors.initials: KC
authors.lastname: Choi
mesh_terms: D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000008:administration & dosage* / Q000494:pharmacology / Q000627:therapeutic use; D048271:Chitosan / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry; D003911:Dextrans / Q000138:chemical synthesis / Q000737:chemistry*; D004317:Doxorubicin / Q000008:administration & dosage* / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use; D004337:Drug Carriers / Q000138:chemical synthesis / Q000737:chemistry*; D005260:Female; D005492:Folic Acid / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry; D058972:Folic Acid Transporters / Q000378:metabolism; D006801:Humans; D007624:KB Cells; D008819:Mice, Nude; D053758:Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D023041:Xenograft Model Antitumor Assays
chemical_list: D000903:Antibiotics, Antineoplastic; D003911:Dextrans; D004337:Drug Carriers; D058972:Folic Acid Transporters; D004317:Doxorubicin; D048271:Chitosan; D005492:Folic Acid