title: Rats fed diets with different energy contribution from fat do not differ in adiposity.
abstract: OBJECTIVE
  To determine whether rats reaching the same body mass, having been fed either a low-fat (LFD) or a high-fat diet (HFD), differ in white adipose tissue (WAT) deposition.
  
  
  METHODS
  In experiment 1, 22 Sprague-Dawley rats of the same age were divided into 11 rats with body mass below the batch median and fed a HFD, and 11 above the median and fed a LFD. In experiment 2, 20 Sprague-Dawley rats of the same age and starting body mass were randomised to either a HFD or LFD. When all groups reached similar final body mass, WAT was quantified using magnetic resonance imaging (MRI), dissection, and plasma leptin.
  
  
  RESULTS
  In experiment 1, both groups reached similar final body mass at the same age; in experiment 2 the HFD group reached similar final body mass earlier than the LFD group. There were no significant differences in WAT as assessed by MRI or leptin between the HFD and LFD groups in both experiments. Dissection revealed a trend for higher retroperitoneal and epididymal adiposity in the HFD groups in both experiments.
  
  
  CONCLUSIONS
  We conclude that at similar body mass, adiposity is independent of the macronutrient composition of the feeding regimen used to achieve it.
authors.affiliation: Section of Investigative Medicine, Imperial College London, UK.
authors.forename: Alexander D
authors.initials: AD
authors.lastname: Miras
authors.forename: Florian
authors.initials: F
authors.lastname: Seyfried
authors.forename: Alkystis
authors.initials: A
authors.lastname: Phinikaridou
authors.forename: Marcelo E
authors.initials: ME
authors.lastname: Andia
authors.forename: Ioannis
authors.initials: I
authors.lastname: Christakis
authors.forename: Alan C
authors.initials: AC
authors.lastname: Spector
authors.forename: René M
authors.initials: RM
authors.lastname: Botnar
authors.forename: Carel W
authors.initials: CW
authors.lastname: le Roux
mesh_terms: D052436:Adipose Tissue, White / Q000378:metabolism*; D050154:Adiposity; D000367:Age Factors; D000818:Animals; D004032:Diet; D018752:Diet, Fat-Restricted; D059305:Diet, High-Fat; D004041:Dietary Fats / Q000008:administration & dosage* / Q000494:pharmacology; D002149:Energy Intake; D004822:Epididymis / Q000378:metabolism; D020738:Leptin / Q000097:blood; D008297:Male; D009765:Obesity / Q000097:blood / Q000209:etiology*; D010537:Peritoneum / Q000378:metabolism; D011897:Random Allocation; D017207:Rats, Sprague-Dawley; D015430:Weight Gain
chemical_list: D004041:Dietary Fats; D020738:Leptin