title: The impact of exogenic testosterone and nortestosterone-decanoate toxicological evaluation using a rat model.
abstract: The impact of exogenic testosterone (T): 1.5 and 3.0 mg/kg.bw) and 19-nortestosterone 17-decanoate (ND): 1.5 and 7.5 mg/kg.bw) in castrated male rats was evaluated based on: (a) weight increase of the androgen target tissues, respecting the Hershberger methodology; (b) the 17α and β-testosterone, 17 α and β-estradiol and 17 α and β-nortestosterone levels using the GC-MS/MS technique; and (c) observation of the serum free thyroxine levels (T4). Results revealed that T and ND significantly increased the weight of androgen target tissues as follows: ND was more influential on seminal vesicles, levator ani-bulbocavernosus muscle (LABC) and Cowper's glands and T (at a dose of 3.0 mg/kg.bw) influenced the weight of the ventral prostate and glans penis. Serum samples analyzed for steroid hormone levels showed the presence of 17β-testosterone, 17β-estradiol and 17β-nor-testosterone, in castrated male rats injected with testosterone and nortestosterone, but no significant differences were found between thyroid responses and thyroid hormone levels. The results of this research proved the disrupting activity of T and ND when administered in high doses and the useful application of the Hershberger bioassay in the case of ND.
authors.affiliation: Pharmacology and Pharmacy Departments, Banat's University of Agricultural Sciences and Veterinary Medicine "King Michael of Romania" from Timişoara, Timiş, Romania.
authors.forename: Romeo Teodor
authors.initials: RT
authors.lastname: Cristina
authors.affiliation: Pharmacology and Pharmacy Departments, Banat's University of Agricultural Sciences and Veterinary Medicine "King Michael of Romania" from Timişoara, Timiş, Romania.
authors.forename: Flavia
authors.initials: F
authors.lastname: Hanganu
authors.affiliation: Pharmacology and Pharmacy Departments, Banat's University of Agricultural Sciences and Veterinary Medicine "King Michael of Romania" from Timişoara, Timiş, Romania.
authors.forename: Eugenia
authors.initials: E
authors.lastname: Dumitrescu
authors.affiliation: Toxicology Department, Banat's University of Agricultural Sciences and Veterinary Medicine "King Michael of Romania" from Timişoara, Timiş, Romania.
authors.forename: Florin
authors.initials: F
authors.lastname: Muselin
authors.affiliation: Biochemistry Department, Banat's University of Agricultural Sciences and Veterinary Medicine "King Michael of Romania" from Timişoara, Timiş, Romania.
authors.forename: Monica
authors.initials: M
authors.lastname: Butnariu
authors.affiliation: National Reference Laboratory for A3 Steroids, Constanţa, Constanţa, Romania.
authors.forename: Adriana
authors.initials: A
authors.lastname: Constantin
authors.affiliation: Drugs Production Department, Romvac Company, Voluntari, Ilfov, Romania.
authors.forename: Viorica
authors.initials: V
authors.lastname: Chiurciu
mesh_terms: D000818:Animals; D002369:Castration; D006728:Hormones / Q000097:blood; D008297:Male; D009277:Nandrolone / Q000031:analogs & derivatives* / Q000633:toxicity; D000077603:Nandrolone Decanoate; D009929:Organ Size / Q000187:drug effects; D051381:Rats; D013739:Testosterone / Q000633:toxicity*
chemical_list: D006728:Hormones; D013739:Testosterone; D009277:Nandrolone; D000077603:Nandrolone Decanoate