title: Low molecular weight polyethylenimine as a transgenic vector for tumor gene therapy.
abstract: We constructed a polymer composed of a series of small molecule polyethylenimine (PEI) using 4-arm polyethylenimine (PEG) as a core for tumor gene therapy. N,N'-carbonyldiimidazole and N-succinimidyl-3-(2-pyridyldithio) propionate were used as chemical connecting reagents to synthesize 4-arm PEG-PEI2000 and 4-arm PEG-PEI2000-MC11. Chemical characterization was performed using (1)H-NMR. The retardation effect of polymers on plasmid DNA was observed using electrophoretic mobility shift and MTT assays to test the toxicity of the polymers. The gene delivery capability of 4-arm PEG-PEI2000 and 4-arm PEG-PEI2000-MC11, and the effect of MC11 were determined by an in vitro gene delivery experiment with human hepatoma HepG2 cells. At a N:P ratio of 3, the 4-arm PEG-PEI2000 could retard successfully plasmid DNA with low toxicity. In experiments in vitro, when the N:P ratio was 30, the gene delivery efficiency of 4-arm PEG-PEI2000 in HepG2 cells was five times that of PEI2000; After connecting ligand MC11, however, the gene delivery efficiency was twice as great. Free MC11 effectively inhibited the gene delivery efficiency of the 4-arm PEG-PEI2000-MC11. Four-arm PEG-PEI2000 has low toxicity and high gene delivery efficiency, and is an effective gene delivery vector after linking ligand MC11.
authors.affiliation: Department of Stomatology.
authors.forename: Z
authors.initials: Z
authors.lastname: Lou
authors.forename: H
authors.initials: H
authors.lastname: Xing
authors.forename: H
authors.initials: H
authors.lastname: Pan
authors.forename: F
authors.initials: F
authors.lastname: Lou
authors.forename: K
authors.initials: K
authors.lastname: Wang
authors.forename: Y
authors.initials: Y
authors.lastname: Fang
authors.forename: W
authors.initials: W
authors.lastname: Han
authors.forename: D
authors.initials: D
authors.lastname: Li
mesh_terms: D018014:Gene Transfer Techniques; D015316:Genetic Therapy; D005822:Genetic Vectors / Q000235:genetics / Q000627:therapeutic use*; D056945:Hep G2 Cells; D006801:Humans; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008970:Molecular Weight; D009369:Neoplasms / Q000235:genetics* / Q000628:therapy; D010957:Plasmids / Q000235:genetics; D011094:Polyethyleneimine; D014162:Transfection / Q000379:methods
chemical_list: D011094:Polyethyleneimine
keywords: FGF; gene delivery; gene therapy; polyethylenimine; receptor; tumor; vector