title: Two-photon AgNP/DNA-TP dye nanosensing conjugate for biothiol probing in live cells.
abstract: A novel silver nanoparticle (AgNP)/DNA-two-photon dye (TP dye) conjugate was fabricated as a two-photon nanoprobe for biothiol imaging in live cells. DNA-templated silver nanoparticles are efficient quenchers and also provide a biocompatible nanoplatform for facile delivery of DNA into living cells. In the presence of biothiols (Cys, Hcy, or GSH), the strong interaction between the thiol group and silver results in the release of TP dye-labeled single-stranded DNA (ssDNA) from the AgNP surface and the subsequent fluorescence emission of the TP dye, thus enabling biothiols to be assayed. Our results reveal that the AgNP/DNA-TP dye nanosensing conjugate not only is a robust, sensitive, and selective sensor for quantitative detection of biothiols in the complex biological environment but also can be efficiently delivered into live cells and act as a "signal-on" sensor for specific, high-contrast imaging of target biomolecules. Our design provides a methodology for the development of future DNA-templated silver nanoparticle-based two-photon fluorescent probes for use in vitro or in vivo as biomolecular sensors for live-cell imaging.
authors.affiliation: State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, China. jishanli@hnu.edu.cn.
authors.forename: Mingli
authors.initials: M
authors.lastname: Liu
authors.forename: Qiao
authors.initials: Q
authors.lastname: Tang
authors.forename: Ting
authors.initials: T
authors.lastname: Deng
authors.forename: Huijuan
authors.initials: H
authors.lastname: Yan
authors.forename: Jishan
authors.initials: J
authors.lastname: Li
authors.forename: Yinhui
authors.initials: Y
authors.lastname: Li
authors.forename: Ronghua
authors.initials: R
authors.lastname: Yang
mesh_terms: D015374:Biosensing Techniques; D004247:DNA / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry*; D015335:Molecular Probes; D036103:Nanotechnology; D012834:Silver / Q000737:chemistry*; D013438:Sulfhydryl Compounds / Q000737:chemistry*
chemical_list: D005456:Fluorescent Dyes; D015335:Molecular Probes; D013438:Sulfhydryl Compounds; D012834:Silver; D004247:DNA