title: Synthesis of Tat tagged and folate modified N-succinyl-chitosan self-assembly nanoparticles as a novel gene vector.
abstract: The purpose of this research was to prepare a novel type of Tat tagged and folate modified N-succinyl-chitosan (Tat-Suc-FA) self-assembly nanoparticles, to provide a new vector for tumor gene therapy. In this study, Tat-Suc-FA polymers was synthesized and characterized using (1)H NMR and FT-IR. The copolymer had a mean diameter of 65 ± 22.6 nm, a zeta potential of 40 ± 0.2 mV. The cytotoxicity assay showed that Tat-Suc-FA polymers were less toxic than chitosan in the tested concentration range (from 2 to 500 μg/ml). Tat-Suc-FA/DNA complexes at various weight ratios were formulated and characterized. Particle sizes of Tat-Suc-FA/DNA complexes were between 54 and 106 nm as determined by dynamic light scattering. Accordingly, Transmission electron microscope photo of Tat-Suc-FA/DNA complexes exhibited a spherical and compact morphology. Zeta potentials of these complexes changed as the weight ratio varied (from 3 to 44 mV). Agarose gel electrophoresis assay showed that Tat-Suc-FA could efficiently condense the DNA, when the weight ratio was above 1.5/1. Together, these results suggest that the low toxic Tat-Suc-FA cationic polymers could be considered for use as a novel type of gene delivery vectors.
authors.affiliation: College of Pharmacy, Guilin Medical University, Guilin 541004, China; First Affiliated Hospital of Jiamusi University, Jiamusi of University, Jiamusi 154000, China. Electronic address: hademan@126.com.
authors.forename: Cheng-yun
authors.initials: CY
authors.lastname: Yan
authors.affiliation: First Affiliated Hospital of Jiamusi University, Jiamusi of University, Jiamusi 154000, China.
authors.forename: Ji-wei
authors.initials: JW
authors.lastname: Gu
authors.affiliation: First Affiliated Hospital of Jiamusi University, Jiamusi of University, Jiamusi 154000, China.
authors.forename: Da-ping
authors.initials: DP
authors.lastname: Hou
authors.affiliation: First Affiliated Hospital of Jiamusi University, Jiamusi of University, Jiamusi 154000, China.
authors.forename: Hong-ying
authors.initials: HY
authors.lastname: Jing
authors.affiliation: First Affiliated Hospital of Jiamusi University, Jiamusi of University, Jiamusi 154000, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.affiliation: First Affiliated Hospital of Jiamusi University, Jiamusi of University, Jiamusi 154000, China.
authors.forename: Yu-zhi
authors.initials: YZ
authors.lastname: Guo
authors.affiliation: Department of Biopharmaceutics, Kyoto Pharmaceutical University, Misasagi, Yamashina-ku, Kyoto 607-8414, Japan.
authors.forename: Hidemasa
authors.initials: H
authors.lastname: Katsumi
authors.affiliation: Department of Biopharmaceutics, Kyoto Pharmaceutical University, Misasagi, Yamashina-ku, Kyoto 607-8414, Japan.
authors.forename: Toshiyasu
authors.initials: T
authors.lastname: Sakane
authors.affiliation: Department of Biopharmaceutics, Kyoto Pharmaceutical University, Misasagi, Yamashina-ku, Kyoto 607-8414, Japan.
authors.forename: Akira
authors.initials: A
authors.lastname: Yamamoto
mesh_terms: D002412:Cations / Q000737:chemistry; D048271:Chitosan / Q000737:chemistry*; D004247:DNA / Q000737:chemistry* / Q000235:genetics; D005492:Folic Acid / Q000737:chemistry* / Q000235:genetics; D018014:Gene Transfer Techniques; D049452:Green Fluorescent Proteins / Q000737:chemistry / Q000235:genetics; D006801:Humans; D053758:Nanoparticles / Q000737:chemistry / Q000627:therapeutic use; D017550:Spectroscopy, Fourier Transform Infrared; D054322:tat Gene Products, Human Immunodeficiency Virus / Q000737:chemistry / Q000235:genetics*
chemical_list: D002412:Cations; C118639:N-succinyl-chitosan; D054322:tat Gene Products, Human Immunodeficiency Virus; D049452:Green Fluorescent Proteins; D004247:DNA; D048271:Chitosan; D005492:Folic Acid
keywords: Folic acid; Gene delivery; N-Succinyl-chitosan; Self-assembly nanoparticles; Tat peptide