title: Alterations of proliferation and differentiation of hippocampal cells in prenatally stressed rats.
abstract: OBJECTIVE
  To clarify the alterations of proliferation and differentiation of hippocampal cells in prenatally stressed rats.
  
  
  METHODS
  We investigated the impact of prenatal restraint stress on the hipocampal cell proliferation in the progeny with 5-bromo-2'-deoxyuridine (BrdU), which is a marker of proliferating cells and their progeny. In addition, we observed the differentiation of neural stem cells (NSCs) with double labeling of BrdU/neurofilament (NF), BrdU/glial fibrillary acidic protein (GFAP) in the hipocampus.
  
  
  RESULTS
  Prenatal stress (PS) increased cell proliferation in the dentate gyrus (DG) only in female and neuron differentiation of newly divided cells in the DG and CA4 in both male and female. Moreover, the NF and GFAP-positive cells, but not the BrdU-positive cells, BrdU/NF and BrdU/GFAP-positive cells, were found frequently in the CA3 and CA1 in the offspring of each group.
  
  
  CONCLUSIONS
  These results possibly suggest a compensatory adaptive response to neuronal damage or loss in hippocampus induced by PS.
authors.affiliation: Shaanxi Institute of Pediatric Diseases, The Affiliated Children Hospital of Xi'an Jiaotong University College of Medicine, Shaanxi 86-710003, PR China.
authors.forename: Hongli
authors.initials: H
authors.lastname: Sun
authors.affiliation: Department of Neonatology, The First Affiliated Hospital of Xi'an Jiaotong University, Shaanxi 86-710061, PR China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Su
authors.affiliation: The Affiliated Children Hospital of Xi'an Jiaotong University College of Medicine, Shaanxi 86-710003, PR China.
authors.forename: Huifang
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Department of Neonatology, The First Affiliated Hospital of Xi'an Jiaotong University, Shaanxi 86-710061, PR China.
authors.forename: Weimin
authors.initials: W
authors.lastname: Liu
authors.affiliation: Department of Neonatology, The First Affiliated Hospital of Xi'an Jiaotong University, Shaanxi 86-710061, PR China.
authors.forename: Huiping
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Department of Neonatology, The First Affiliated Hospital of Xi'an Jiaotong University, Shaanxi 86-710061, PR China.
authors.forename: Ding
authors.initials: D
authors.lastname: Ding
authors.affiliation: Shaanxi Province Biomedicine Key Laboratory, College of Life Sciences, Northwest University, Shaanxi 86-710069, PR China.
authors.forename: Zhongliang
authors.initials: Z
authors.lastname: Zhu
authors.affiliation: Department of Neonatology, The First Affiliated Hospital of Xi'an Jiaotong University, Shaanxi 86-710061, PR China; The Affiliated Children Hospital of Xi'an Jiaotong University College of Medicine, Shaanxi 86-710003, PR China. Electronic address: huili@mail.xjtu.edu.cn.
authors.forename: Hui
authors.initials: H
authors.lastname: Li
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D049109:Cell Proliferation; D005260:Female; D006624:Hippocampus / Q000503:physiopathology*; D008297:Male; D058953:Neural Stem Cells / Q000502:physiology*; D009474:Neurons / Q000502:physiology*; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects / Q000503:physiopathology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012149:Restraint, Physical; D012737:Sex Factors; D013315:Stress, Psychological / Q000503:physiopathology*
keywords: BrdU; Glial fibrillary acidic protein; Hippocampus; Neurofilament; Prenatal stress