title: A multiscale three-zone reactive mixing model for engineering a scale separation in enzymatic hydrolysis of cellulose.
abstract: This multiscale three-zone reactive mixing model provides a theoretical framework for engineering a scale separation in batch enzymatic hydrolysis of cellulose to strategize significant leaps in glucose yields. Formulated using the Liapunov-Schmidt method of the classical bifurcation theory, our model explores the multiscale spatiotemporal dynamics between the fundamental processes of macromixing (convection) and micromixing (diffusion) of the enzymes (Endoglucanase, Exoglucanase, β-glucasidase) and reducing sugars, adsorption and desorption of enzymes on the solid cellulosic substrates, and the product-inhibited liquid and solid phase enzymatic reactions that depolymerize microcrystalline cellulose (Avicel). The model is validated for a range of substrate loadings (2-5%) using our experimental results for the two asymptotic cases of no mixing and continuous mixing, as well as for the macro/micro scale-separated optimal mixing strategy that increases the glucose yield by up to 26% by macromixing completely for an initial period followed by micromixing for the remaining duration of the hydrolysis.
authors.affiliation: Department of Chemical Engineering, Indian Institute of Technology, Kharagpur 721302, India. Electronic address: saikat@che.iitkgp.ernet.in.
authors.forename: Saikat
authors.initials: S
authors.lastname: Chakraborty
authors.affiliation: Department of Chemical Engineering, Indian Institute of Technology, Kharagpur 721302, India.
authors.forename: Satyanarayana
authors.initials: S
authors.lastname: Raju
authors.affiliation: Department of Chemical Engineering, Indian Institute of Technology, Kharagpur 721302, India.
authors.forename: Ramendra Kishor
authors.initials: RK
authors.lastname: Pal
mesh_terms: D002480:Cellulase / Q000737:chemistry*; D002482:Cellulose / Q000737:chemistry*; D003198:Computer Simulation; D017076:Computer-Aided Design; D004789:Enzyme Activation; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006868:Hydrolysis; D008956:Models, Chemical; D009097:Multienzyme Complexes / Q000737:chemistry*; D010865:Pilot Projects; D012212:Rheology / Q000295:instrumentation* / Q000379:methods*
chemical_list: D009097:Multienzyme Complexes; D002482:Cellulose; D002480:Cellulase
keywords: Bioethanol; Cellulose; Enzymatic hydrolysis; Mixing; Multiscale Model