title: Evaluating the efficiency of spectral resolution of univariate methods manipulating ratio spectra and comparing to multivariate methods: an application to ternary mixture in common cold preparation.
abstract: Simple, accurate, and selective methods have been developed and validated for simultaneous determination of a ternary mixture of Chlorpheniramine maleate (CPM), Pseudoephedrine HCl (PSE) and Ibuprofen (IBF), in tablet dosage form. Four univariate methods manipulating ratio spectra were applied, method A is the double divisor-ratio difference spectrophotometric method (DD-RD). Method B is double divisor-derivative ratio spectrophotometric method (DD-RD). Method C is derivative ratio spectrum-zero crossing method (DRZC), while method D is mean centering of ratio spectra (MCR). Two multivariate methods were also developed and validated, methods E and F are Principal Component Regression (PCR) and Partial Least Squares (PLSs). The proposed methods have the advantage of simultaneous determination of the mentioned drugs without prior separation steps. They were successfully applied to laboratory-prepared mixtures and to commercial pharmaceutical preparation without any interference from additives. The proposed methods were validated according to the ICH guidelines. The obtained results were statistically compared with the official methods where no significant difference was observed regarding both accuracy and precision.
authors.affiliation: Analytical Chemistry Department, Faculty of Pharmacy, Cairo University, Kasr-El Aini Street, 11562 Cairo, Egypt.
authors.forename: Azza Aziz
authors.initials: AA
authors.lastname: Moustafa
authors.affiliation: Analytical Chemistry Department, Faculty of Pharmacy, Deraya University, 14511 Cairo, Egypt.
authors.forename: Hesham
authors.initials: H
authors.lastname: Salem
authors.affiliation: Analytical Chemistry Department, Faculty of Pharmacy, Cairo University, Kasr-El Aini Street, 11562 Cairo, Egypt.
authors.forename: Maha
authors.initials: M
authors.lastname: Hegazy
authors.affiliation: Analytical Chemistry Department, Faculty of Pharmacy, October University for Modern Sciences and Arts (MSA), 11787 6th October City, Egypt. Electronic address: dr.omniali@gmail.com.
authors.forename: Omnia
authors.initials: O
authors.lastname: Ali
mesh_terms: D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000032:analysis*; D002744:Chlorpheniramine / Q000032:analysis*; D003139:Common Cold / Q000188:drug therapy; D004338:Drug Combinations; D006634:Histamine H1 Antagonists / Q000032:analysis*; D007052:Ibuprofen / Q000032:analysis*; D016018:Least-Squares Analysis; D057230:Limit of Detection; D015999:Multivariate Analysis; D014663:Nasal Decongestants / Q000032:analysis*; D054199:Pseudoephedrine / Q000032:analysis*; D013053:Spectrophotometry / Q000379:methods; D013607:Tablets
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D004338:Drug Combinations; D006634:Histamine H1 Antagonists; D014663:Nasal Decongestants; D013607:Tablets; D002744:Chlorpheniramine; D054199:Pseudoephedrine; D007052:Ibuprofen
keywords: Chlorpheniramine maleate; Ibuprofen; Multivariate methods; Pseudoephedrine HCl; Ratio spectra; Univariate methods