title: Variation of nonylphenol-degrading gene abundance and bacterial community structure in bioaugmented sediment microcosm.
abstract: Nonylphenol (NP) can accumulate in river sediment. Bioaugmentation is an attractive option to dissipate heavy NP pollution in river sediment. In this study, two NP degraders were isolated from crude oil-polluted soil and river sediment. Microcosms were constructed to test their ability to degrade NP in river sediment. The shift in the proportion of NP-degrading genes and bacterial community structure in sediment microcosms were characterized using quantitative PCR assay and terminal restriction fragment length polymorphism analysis, respectively. Phylogenetic analysis indicated that the soil isolate belonged to genus Stenotrophomonas, while the sediment isolate was a Sphingobium species. Both of them could almost completely clean up a high level of NP in river sediment (150 mg/kg NP) in 10 or 14 days after inoculation. An increase in the proportion of alkB and sMO genes was observed in sediment microcosms inoculated with Stenotrophomonas strain Y1 and Sphingobium strain Y2, respectively. Moreover, bioaugmentation using Sphingobium strain Y2 could have a strong impact on sediment bacterial community structure, while inoculation of Stenotrophomonas strain Y1 illustrated a weak impact. This study can provide some new insights towards NP biodegradation and bioremediation.
authors.affiliation: State Key Joint Laboratory of Environmental Simulation and Pollution Control, College of Environmental Sciences and Engineering, Peking University, Beijing, 100871, China.
authors.forename: Zhao
authors.initials: Z
authors.lastname: Wang
authors.forename: Yuyin
authors.initials: Y
authors.lastname: Yang
authors.forename: Weimin
authors.initials: W
authors.lastname: Sun
authors.forename: Yu
authors.initials: Y
authors.lastname: Dai
authors.forename: Shuguang
authors.initials: S
authors.lastname: Xie
mesh_terms: D001673:Biodegradation, Environmental; D019015:Geologic Sediments / Q000382:microbiology*; D059013:Microbial Consortia / Q000235:genetics*; D059392:Petroleum Pollution; D010636:Phenols / Q000378:metabolism*; D010802:Phylogeny; D012150:Polymorphism, Restriction Fragment Length; D045483:Rivers; D012987:Soil; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000378:metabolism*
chemical_list: D010636:Phenols; D012987:Soil; D012989:Soil Pollutants; C025256:nonylphenol