title: [Immunogenicity and safety of vaccine preparations based on circulating Bordetella pertussis strains].
abstract: OBJECTIVE
  Study specific activity and safety ofvaccine preparations based on circulating B. pertussis strains with currently predominating allele variants of pertussis toxin (ptxA1) and pertactin (prn2) genes.
  
  
  METHODS
  B. pertussis strains isolated from pertussis patients in Moscow in 2001-2010 were grown in dense and liquid media. The content of separate antigens in B. pertussis strains was determined by EIA. Immunogenicity and safety of the preparations was determined in F1(CBAxC57B16) line mice.
  
  
  RESULTS
  All the studied circulating B. pertussis strains expressed pertussis toxin (PT), filamentous hemagglutinin (FHA) and agglutinogens corresponding to the serovar. Whole-cell and acellular pertussis vaccines were prepared based on the circulating strains, and a highly productive recently isolated toxigenic B.pertussis strain that could be used for production ofpertussis vaccines was selected as a result of studies ofimmunogenic, toxic and sensibilizing properties.
  
  
  CONCLUSIONS
  Vaccine preparations based on a B. pertussis strain adapted to growth in liquid media with pertussis toxin and pertactin ptxAl1 - prn2 gene allele variation characteristic for contemporary population are specifically active and safe.
authors.forename: E M
authors.initials: EM
authors.lastname: Zaĭtsev
authors.forename: N Iu
authors.initials: NIu
authors.lastname: Mertsalova
authors.forename: M V
authors.initials: MV
authors.lastname: Britsina
authors.forename: M N
authors.initials: MN
authors.lastname: Ozeretskovskaia
authors.forename: I G
authors.initials: IG
authors.lastname: Bazhanova
authors.forename: A S
authors.initials: AS
authors.lastname: Shinkarev
authors.forename: A V
authors.initials: AV
authors.lastname: Poddubikov
mesh_terms: D000483:Alleles; D000818:Animals; D001886:Bordetella pertussis / Q000187:drug effects* / Q000276:immunology / Q000472:pathogenicity; D006801:Humans; D051379:Mice; D009031:Moscow; D037342:Pertussis Toxin / Q000276:immunology; D010567:Pertussis Vaccine / Q000276:immunology*; D014917:Whooping Cough / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control*
chemical_list: D010567:Pertussis Vaccine; D037342:Pertussis Toxin