title: Paclitaxel-incorporated nanoparticles using block copolymers composed of poly(ethylene glycol)/poly(3-hydroxyoctanoate).
abstract: Block copolymers composed of poly(3-hydroxyoctanoate) (PHO) and methoxy poly(ethylene glycol) (PEG) were synthesized to prepare paclitaxel-incorporated nanoparticle for antitumor drug delivery. In a (1)H-NMR study, chemical structures of PHO/PEG block copolymers were confirmed and their molecular weight (M.W.) was analyzed with gel permeation chromatography (GPC). Paclitaxel as a model anticancer drug was incorporated into the nanoparticles of PHO/PEG block copolymer. They have spherical shapes and their particle sizes were less than 100 nm. In a (1)H-NMR study in D2O, specific peaks of PEG solely appeared while peaks of PHO disappeared, indicating that nanoparticles have core-shell structures. The higher M.W. of PEG decreased loading efficiency and particle size. The higher drug feeding increased drug contents and average size of nanoparticles. In the drug release study, the higher M.W. of PEG block induced the acceleration of drug release rate. The increase in drug contents induced the slow release rate of drug. In an antitumor activity study in vitro, paclitaxel nanoparticles have practically similar anti-proliferation activity against HCT116 human colon carcinoma cells. In an in vivo animal study using HCT116 colon carcinoma cell-bearing mice, paclitaxel nanoparticles have enhanced antitumor activity compared to paclitaxel itself. Therefore, paclitaxel-incorporated nanoparticles of PHO/PEG block copolymer are a promising vehicle for antitumor drug delivery.
authors.affiliation: Department of Surgery, National Research & Development Center for Hepatobiliary Cancer, Pusan National University Yangsan Hospital, Gyeongnam 626-770, Republic of Korea.
authors.forename: Hyun Yul
authors.initials: HY
authors.lastname: Kim
authors.affiliation: Department of Surgery, National Research & Development Center for Hepatobiliary Cancer, Pusan National University Yangsan Hospital, Gyeongnam 626-770, Republic of Korea.
authors.forename: Je Ho
authors.initials: JH
authors.lastname: Ryu
authors.affiliation: Department of Surgery, National Research & Development Center for Hepatobiliary Cancer, Pusan National University Yangsan Hospital, Gyeongnam 626-770, Republic of Korea.
authors.forename: Chong Woo
authors.initials: CW
authors.lastname: Chu
authors.affiliation: Department of Surgery, National Research & Development Center for Hepatobiliary Cancer, Pusan National University Yangsan Hospital, Gyeongnam 626-770, Republic of Korea.
authors.forename: Gyung Mo
authors.initials: GM
authors.lastname: Son
authors.affiliation: Biomedical Research Institute, Pusan National University Hospital, Pusan 602-739, Republic of Korea.
authors.forename: Young-Il
authors.initials: YI
authors.lastname: Jeong
authors.affiliation: Biomedical Research Institute, Pusan National University Hospital, Pusan 602-739, Republic of Korea.
authors.forename: Tae-Won
authors.initials: TW
authors.lastname: Kwak
authors.affiliation: Biomedical Research Institute, Pusan National University Hospital, Pusan 602-739, Republic of Korea.
authors.forename: Do Hyung
authors.initials: DH
authors.lastname: Kim
authors.affiliation: Biomedical Research Institute, Pusan National University Hospital, Pusan 602-739, Republic of Korea.
authors.forename: Chung-Wook
authors.initials: CW
authors.lastname: Chung
authors.affiliation: Department of Microbiology, Chungnam National University, Daejeon 305-764, Korea.
authors.forename: Young Ha
authors.initials: YH
authors.lastname: Rhee
authors.affiliation: Biomedical Research Institute, Pusan National University Hospital, Pusan 602-739, Republic of Korea ; National Research & Development Center for Hepatobiliary Cancer, Pusan National University Yangsan Hospital, Gyeongnam 626-770, Republic of Korea.
authors.forename: Dae Hwan
authors.initials: DH
authors.lastname: Kang
authors.affiliation: Department of Internal Medicine, Pusan National University Yangsan Hospital, Gyeongnam 626-770, Republic of Korea.
authors.forename: Hyung Wook
authors.initials: HW
authors.lastname: Kim
keywords: Block copolymer; Drug delivery; Nanoparticles; Poly(3-hydroxyoctanoate)