title: Myelinating cocultures of purified oligodendrocyte lineage cells and retinal ganglion cells.
abstract: In this article, we introduce methods for generating rapidly myelinating cocultures with reaggregates of purified retinal ganglion cells and optic nerve oligodendrocyte precursor cells. This coculture system facilitates the study of complex central nervous system neuronal-glial interactions and myelination. It enables control of the extracellular environment and allows the use of transfected, virally infected, mutant, or knockout neurons and/or glial cell types. It is therefore possible to assess the role of various signaling pathways and genes in myelination and node of Ranvier formation.
authors.affiliation: Department of Neurobiology, Stanford University School of Medicine, Stanford, California 94305-5125.
authors.forename: Trent A
authors.initials: TA
authors.lastname: Watkins
authors.affiliation: Department of Neurobiology, Stanford University School of Medicine, Stanford, California 94305-5125.
authors.forename: Anja R
authors.initials: AR
authors.lastname: Scholze
mesh_terms: D000818:Animals; D000941:Antigens / Q000378:metabolism; D001369:Axons / Q000502:physiology; D002454:Cell Differentiation; D002478:Cells, Cultured; D018920:Coculture Techniques; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D004676:Myelin Basic Protein / Q000378:metabolism*; D009186:Myelin Sheath / Q000502:physiology*; D009836:Oligodendroglia / Q000166:cytology / Q000502:physiology*; D011509:Proteoglycans / Q000378:metabolism; D012160:Retina / Q000166:cytology*; D012165:Retinal Ganglion Cells / Q000502:physiology*; D013234:Stem Cells / Q000166:cytology; D016875:tau Proteins / Q000378:metabolism; D000099216:Chondroitin Sulfate Proteoglycan 4
chemical_list: D000941:Antigens; D004791:Enzyme Inhibitors; D005904:Glial Fibrillary Acidic Protein; D008869:Microtubule-Associated Proteins; D004676:Myelin Basic Protein; D011509:Proteoglycans; D016875:tau Proteins; D000099216:Chondroitin Sulfate Proteoglycan 4; C079240:Mtap2 protein, mouse