title: A comparative analysis of isotonic versus hypertonic solution volume replacement in septic rats.
abstract: OBJECTIVE
  Sepsis severity and mortality risk require aggressive therapy which includes hemodynamic support. The best fluid for volume replacement, however, is controversial. This study aimed to compare 0.9% isotonic saline solution versus 7.5% hypertonic saline solution as volume replacement fluid in sepsis induced by cecum ligation plus puncture rats.
  
  
  METHODS
  This experimental trial included 30 rats divided into three groups: Control group (CG, n = 10), isotonic (ISG, n = 10) and hypertonic solution (HSG, n = 10). Fifteen hours after cecum ligation and puncture, all animals underwent respiratory rate, mean arterial pressure, renal and hepatic blood flow and weight evaluation, plus blood collection for TNF-α measurement. The ISG and HSG treatment groups received volume replacement 60 minutes before the procedure with either 0.9% or 7.5% saline solution, respectively.
  
  
  RESULTS
  Two animals died. Significant differences were found for the animals' mean weight after 15 hours (p=0.018), particularly relevant when ISG and HSG are compared (p=0.003). Renal blood flow was also significantly different for the CG versus HSG (p=0.002) and CG versus ISG (p=0.008), but not significantly different for ISG versus HSG. No mean arterial pressure improvement was found for HSG (0.054). Other variables were not significant.
  
  
  CONCLUSIONS
  Although no mean blood pressure, hepatic flow or TNF-α improvements were detected, the rats with sepsis 15 hours after cecum ligation and puncture showed significantly increased renal blood flow which was 0.9% isotonic saline solution or of 7.5% hypertonic solution use independent.
authors.affiliation: Pontifícia Universidade Católica do Paraná, Curitiba, PR, Brasil.
authors.forename: Talita Rechetelo
authors.initials: TR
authors.lastname: Strano
authors.affiliation: Pontifícia Universidade Católica do Paraná, Curitiba, PR, Brasil.
authors.forename: Sérgio Luiz
authors.initials: SL
authors.lastname: Rocha
authors.affiliation: Pontifícia Universidade Católica do Paraná, Curitiba, PR, Brasil.
authors.forename: Anna Carolina Botti de
authors.initials: AC
authors.lastname: Oliveira
authors.affiliation: Pontifícia Universidade Católica do Paraná, Curitiba, PR, Brasil.
authors.forename: Débora
authors.initials: D
authors.lastname: Bega
authors.affiliation: Pontifícia Universidade Católica do Paraná, Curitiba, PR, Brasil.
authors.forename: Guilherme
authors.initials: G
authors.lastname: Zammar
authors.affiliation: Pontifícia Universidade Católica do Paraná, Curitiba, PR, Brasil.
authors.forename: Misael Gomes
authors.initials: MG
authors.lastname: Barbosa