title: Recent extensions to native chemical ligation for the chemical synthesis of peptides and proteins.
abstract: Native chemical ligation continues to play a pivotal role in the synthesis of increasingly complex peptide and protein targets twenty years after its initial report. This opinion article will highlight a number of recent, powerful extensions of the technology that have expanded the scope of the reaction, accelerated ligation rates, enabled chemoselective post-ligation modifications, and streamlined the ligation of multiple peptide fragments. These advances have facilitated the synthesis of a number of impressive protein targets to date and hold great promise for the continued application of native chemical ligation for the detailed study of protein structure and function.
authors.affiliation: School of Chemistry, The University of Sydney, Sydney, NSW 2006, Australia.
authors.forename: Lara R
authors.initials: LR
authors.lastname: Malins
authors.affiliation: School of Chemistry, The University of Sydney, Sydney, NSW 2006, Australia. Electronic address: richard.payne@sydney.edu.au.
authors.forename: Richard J
authors.initials: RJ
authors.lastname: Payne
mesh_terms: D000215:Acylation; D000596:Amino Acids / Q000138:chemical synthesis / Q000737:chemistry; D008958:Models, Molecular; D010455:Peptides / Q000138:chemical synthesis* / Q000737:chemistry; D011506:Proteins / Q000138:chemical synthesis* / Q000737:chemistry; D018036:Selenium Compounds / Q000138:chemical synthesis / Q000737:chemistry; D013438:Sulfhydryl Compounds / Q000138:chemical synthesis / Q000737:chemistry; D013455:Sulfur / Q000737:chemistry
chemical_list: D000596:Amino Acids; D010455:Peptides; D011506:Proteins; D018036:Selenium Compounds; D013438:Sulfhydryl Compounds; C442270:selenol; D013455:Sulfur