title: Activated protein C resistance testing for factor V Leiden.
abstract: Activated protein C resistance assays can detect factor V Leiden with high accuracy, depending on the method used. Factor Xa inhibitors such as rivaroxaban and direct thrombin inhibitors including dabigatran, argatroban, and bivalirudin can cause falsely normal results. Lupus anticoagulants can cause incorrect results in most current assays. Assays that include dilution into factor V-deficient plasma are needed to avoid interference from factor deficiencies or elevations, which can arise from a wide variety of conditions such as warfarin, liver dysfunction, or pregnancy. The pros and cons of the currently available assays are discussed.
authors.affiliation: Department of Pathology, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts.
authors.forename: Stephan
authors.initials: S
authors.lastname: Kadauke
authors.forename: Bernard
authors.initials: B
authors.lastname: Khor
authors.forename: Elizabeth M
authors.initials: EM
authors.lastname: Van Cott
mesh_terms: D020016:Activated Protein C Resistance / Q000097:blood / Q000175:diagnosis*; D000328:Adult; D000991:Antithrombins / Q000737:chemistry; D001120:Arginine / Q000031:analogs & derivatives; D001562:Benzimidazoles / Q000737:chemistry; D001681:Biological Assay / Q000592:standards*; D001780:Blood Coagulation Tests; D002648:Child; D000069604:Dabigatran; D005165:Factor V / Q000032:analysis* / Q000378:metabolism; D015951:Factor Xa / Q000378:metabolism; D005189:False Positive Reactions; D005260:Female; D006629:Hirudins / Q000737:chemistry; D006801:Humans; D016682:Lupus Coagulation Inhibitor / Q000737:chemistry; D009025:Morpholines / Q000737:chemistry; D010446:Peptide Fragments / Q000737:chemistry; D010875:Pipecolic Acids / Q000737:chemistry; D011247:Pregnancy; D011486:Protein C / Q000378:metabolism*; D011994:Recombinant Proteins / Q000737:chemistry; D000069552:Rivaroxaban; D013449:Sulfonamides; D013876:Thiophenes / Q000737:chemistry; D014859:Warfarin / Q000737:chemistry; D015091:beta-Alanine / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D000991:Antithrombins; D001562:Benzimidazoles; D006629:Hirudins; D016682:Lupus Coagulation Inhibitor; D009025:Morpholines; D010446:Peptide Fragments; D010875:Pipecolic Acids; D011486:Protein C; D011994:Recombinant Proteins; D013449:Sulfonamides; D013876:Thiophenes; C095381:factor V Leiden; D015091:beta-Alanine; D014859:Warfarin; D005165:Factor V; D001120:Arginine; D000069552:Rivaroxaban; D015951:Factor Xa; D000069604:Dabigatran; C031942:argatroban; C074619:bivalirudin