title: Analysis of the role of myosins in targeting proteins to plasmodesmata.
abstract: Plasmodesmata (PD) are dynamic cell wall microchannels that facilitate the intercellular trafficking of RNA and protein macromolecules playing cell nonautonomous roles in the orchestration of plant development, growth, and plant defense. The trafficking of macromolecules and organelles within cells depends on cytoskeletal components and their associated motor proteins. Plant viruses evolved to hijack this transport system to move their infectious genomes to PD. Current efforts concentrate on dissecting the role of specific myosin motors in transporting plant or viral proteins to the channels. Here we describe a method that addresses the role of specific myosins by expression of myosin tails that cause the repression of myosin activity in a dominant-negative manner. As an example, we explain the use of myosin tails from Nicotiana benthamiana to address the role of N. benthamiana myosins in the targeting of PLASMODESMATA-LOCATED PROTEIN 1 (PDLP1) to PD.
authors.affiliation: Department of Biology-Plant Biology, University of Fribourg, CH-1700, Fribourg, Switzerland.
authors.forename: Martin
authors.initials: M
authors.lastname: Di Donato
authors.forename: Khalid
authors.initials: K
authors.lastname: Amari
mesh_terms: D016960:Agrobacterium tumefaciens / Q000235:genetics* / Q000378:metabolism; D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D005822:Genetic Vectors; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D047908:Intracellular Signaling Peptides and Proteins; D018613:Microscopy, Confocal; D009218:Myosins / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism; D010446:Peptide Fragments / Q000235:genetics / Q000378:metabolism; D018515:Plant Leaves / Q000235:genetics* / Q000378:metabolism; D030821:Plants, Genetically Modified; D031425:Plasmodesmata / Q000235:genetics* / Q000378:metabolism; D021381:Protein Transport; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D014026:Nicotiana / Q000235:genetics* / Q000378:metabolism
chemical_list: D029681:Arabidopsis Proteins; D002352:Carrier Proteins; D047908:Intracellular Signaling Peptides and Proteins; C528450:PDLP1a protein, Arabidopsis; D010446:Peptide Fragments; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins; D009218:Myosins