title: Adsorption of fibronectin on salt-etched polyelectrolyte multilayers and its roles in mediating the adhesion and migration of vascular smooth muscle cells.
abstract: Protein adsorption on biomaterials strongly mediates the subsequent cell responses. Here adsorption of fibronectin (Fn) on salt-treated PEI(PSS/PDDA)7 multilayers was characterized. The amounts of adsorbed Fn increased linearly along with the increase of thickness of multilayers pretreated with 1 M and 5 M NaCl solutions (Multilayer-1M and 5M), but was independent on the thickness of Multilayer-3M. The normalized relative RGD activity of Fn were significantly higher on the Multilayer-3M than on others. By comparison of cellular behaviors of VSMCs being cultured in the normal and Fn-depleted serum-containing medium, the significant role of Fn on modulating the behaviors of VSMCs was verified. The Fn adsorption model and its role on linking the biomaterials surface to the VSMCs behaviors are proposed.
authors.affiliation: MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou, 310027, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Deng
authors.forename: Mingcong
authors.initials: M
authors.lastname: Sun
authors.forename: Shasha
authors.initials: S
authors.lastname: Wang
authors.forename: Lulu
authors.initials: L
authors.lastname: Han
authors.forename: Zhengwei
authors.initials: Z
authors.lastname: Mao
authors.forename: Dan
authors.initials: D
authors.lastname: Li
authors.forename: Hong
authors.initials: H
authors.lastname: Chen
authors.forename: Changyou
authors.initials: C
authors.lastname: Gao
mesh_terms: D000327:Adsorption; D002448:Cell Adhesion / Q000187:drug effects*; D002460:Cell Line; D002465:Cell Movement / Q000187:drug effects*; D042783:Endothelial Cells / Q000187:drug effects / Q000502:physiology*; D004797:Enzyme-Linked Immunosorbent Assay; D005353:Fibronectins / Q000737:chemistry / Q000494:pharmacology*; D006801:Humans; D018625:Microscopy, Atomic Force; D008956:Models, Chemical; D009131:Muscle, Smooth, Vascular / Q000166:cytology*; D011108:Polymers / Q000737:chemistry*; D044968:Regenerative Medicine / Q000379:methods; D012965:Sodium Chloride; D017550:Spectroscopy, Fourier Transform Infrared; D023822:Tissue Engineering / Q000379:methods*
chemical_list: D005353:Fibronectins; D011108:Polymers; D012965:Sodium Chloride
keywords: biomaterial surfaces; cell adhesion and migration; fibronectin; multilayers; protein adsorption