title: VEGF levels in diagnosis of vasculitic neuropathy.
abstract: BACKGROUND
  Vascular endothelial growth factor (VEGF) is secreted from endothelial cells and pericytes in response to hypoxia. It induces angiogenesis and microvascular hyperpermeability. We observe serum VEGF concentrations in some patients with vasculitic neuropathy.
  
  
  METHODS
  Plasma VEGF was measured using GenWay's human VEGF ELISA Kit, which is based on standard sandwich enzyme-linked immune-sorbent assay technology. Human VEGF specific-specific monoclonal antibodies are precoated onto 96-well plates. The human specific detection polyclonal antibodies are biotinylated. The test samples and biotinylated detection antibodies were added to the wells subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. The VEGF levels were measured in 5 patients with vasculitic neuropathy and 8 healthy controls.
  
  
  RESULTS
  Plasma VEGF was higher in subjects with vasculitic neuropathy as compared to controls. In the control group, we obtained VEGF levels from 9.8 pg/mL up to 15 pg/mL with an average of 11.6 pg/mL for males and 8.9 pg/mL up to 14.5 pg/mL with average of 11.2 pg/mL for females. In female patients with vasculitic neuropathy the plasma VEGF levels were between 79.9 pg/mL and 111.2 pg/mL, with an average of 92.375 pg/mL. We had one case with vasculitic neuropathy in men, in which the plasma VEGF level was 102.9 pg/mL.
  
  
  CONCLUSIONS
  The results from our study indicate that plasma VEGF levels are significantly associated with vasculitic neuropathy and may be used to predict this disease.
authors.forename: Victor
authors.initials: V
authors.lastname: Manolov
authors.forename: Iulia
authors.initials: I
authors.lastname: Petrova
authors.forename: Vasil
authors.initials: V
authors.lastname: Vasilev
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000097:blood; D016022:Case-Control Studies; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D010523:Peripheral Nervous System Diseases / Q000097:blood* / Q000175:diagnosis; D010488:Polyarteritis Nodosa / Q000097:blood / Q000175:diagnosis; D011237:Predictive Value of Tests; D011933:Reagent Kits, Diagnostic; D015854:Up-Regulation; D042461:Vascular Endothelial Growth Factor A / Q000097:blood*; D014657:Vasculitis / Q000097:blood* / Q000175:diagnosis
chemical_list: D015415:Biomarkers; D011933:Reagent Kits, Diagnostic; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A