title: Platelet lysate embedded scaffolds for skin regeneration.
abstract: BACKGROUND
  The work presents the development of acellular scaffolds extemporaneously embedded with platelet lysate (PL), as an innovative approach in the field of tissue regeneration/reparation. PL embedded scaffolds should have a tridimensional architecture to support cell migration and growth, in order to restore skin integrity. For this reason, chondroitin sulfate (CS) was associated with sodium alginate (SA) to prepare highly porous systems.
  
  
  METHODS
  The developed scaffolds were characterized for chemical stability to γ-radiation, morphology, hydration and mechanical properties. Moreover, the capability of fibroblasts and endothelial cells to populate the scaffold was evaluated by means of proliferation test 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and confocal laser scanning microscopy study. The scaffolds, not altered by sterilization, were characterized by limited swelling and high flexibility, by foam-like structure with bubbles that formed a high surface area and irregular texture suitable for cell adhesion.
  
  
  RESULTS
  Cell growth and scaffold population were evident on the bubble surface, where the cells appeared anchored to the scaffold structure.
  
  
  CONCLUSIONS
  Scaffold network based on CS and SA demonstrated to be an effective support to enhance and to allow fibroblasts and endothelial cells (human umbilical vein endothelial cells, HUVEC) adhesion and proliferation. In particular, it could be hypothesized that cell adhesion was facilitated by the synergic effect of PL and CS. Although further in vivo evaluation is needed, on the basis of in vitro results, PL embedded scaffolds seem promising systems for skin wound healing.
authors.affiliation: University of Pavia, Department of Drug Sciences , Viale Taramelli 12, 27100 Pavia , Italy +39 0382 987357 ; +39 0382 422975 ; giuseppina.sandri@unipv.it.
authors.forename: Giuseppina
authors.initials: G
authors.lastname: Sandri
authors.forename: Maria Cristina
authors.initials: MC
authors.lastname: Bonferoni
authors.forename: Silvia
authors.initials: S
authors.lastname: Rossi
authors.forename: Franca
authors.initials: F
authors.lastname: Ferrari
authors.forename: Michela
authors.initials: M
authors.lastname: Mori
authors.forename: Marila
authors.initials: M
authors.lastname: Cervio
authors.forename: Federica
authors.initials: F
authors.lastname: Riva
authors.forename: Ioannis
authors.initials: I
authors.lastname: Liakos
authors.forename: Athanassia
authors.initials: A
authors.lastname: Athanassiou
authors.forename: Francesca
authors.initials: F
authors.lastname: Saporito
authors.forename: Lara
authors.initials: L
authors.lastname: Marini
authors.forename: Carla
authors.initials: C
authors.lastname: Caramella
mesh_terms: D000464:Alginates / Q000737:chemistry; D001792:Blood Platelets / Q000378:metabolism*; D002448:Cell Adhesion / Q000502:physiology; D049109:Cell Proliferation / Q000502:physiology; D005347:Fibroblasts / Q000378:metabolism; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D012038:Regeneration / Q000502:physiology*; D012867:Skin / Q000378:metabolism*; D054457:Tissue Scaffolds; D014945:Wound Healing / Q000502:physiology
chemical_list: D000464:Alginates; D006603:Hexuronic Acids; D020723:Glucuronic Acid
keywords: cell proliferation; chondroitin sulfate; platelet lysate; sodium alginate; tridimensional acellular scaffolds