title: The protective role of ferulic acid on sepsis-induced oxidative damage in Wistar albino rats.
abstract: Oxidative stress has an important role in the development of sepsis-induced multiorgan failure. Ferulic acid (FA), a well-established natural antioxidant, has several pharmacological activities including anti-inflammatory, anticancer and hepatoprotective. This study aimed to investigate the effects of FA on sepsis-induced oxidative damage in Wistar albino rats. Sepsis-induced DNA damage in the lymphocytes, liver and kidney cells of rats were evaluated by comet assay with and without formamidopyrimidine DNA glycosylase (Fpg). The oxidative stress parameters such as superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activities and total glutathione (GSH) and malondialdehyde (MDA) levels were also measured. It is found that DNA damage in sepsis+FA-treated group was significantly lower than the sepsis group. FA treatment also decreased the MDA levels and increased the GSH levels and SOD and GSH-Px activities in the sepsis-induced rats. It seems that FA might have ameliorative effects against sepsis-induced oxidative damage.
authors.affiliation: Department of Pharmaceutical Toxicology, Faculty of Pharmacy, Hacettepe University, 06100 Ankara, Turkey.
authors.forename: Merve
authors.initials: M
authors.lastname: Bacanlı
authors.affiliation: Department of Pharmaceutical Toxicology, Faculty of Pharmacy, Hacettepe University, 06100 Ankara, Turkey.
authors.forename: Sevtap
authors.initials: S
authors.lastname: Aydın
authors.affiliation: Department of Biology, Faculty of Science, Gazi University, 06500 Ankara, Turkey.
authors.forename: Gökçe
authors.initials: G
authors.lastname: Taner
authors.affiliation: Department of Pharmaceutical Toxicology, Faculty of Pharmacy, Hacettepe University, 06100 Ankara, Turkey; Department of Pharmaceutical Toxicology, Faculty of Pharmacy, Çukurova University, Sarıçam, 01330 Adana, Turkey.
authors.forename: Hatice Gül
authors.initials: HG
authors.lastname: Göktaş
authors.affiliation: Department of Surgery, Faculty of Kastamonu Medicine, Hacettepe University, 06100 Ankara, Turkey.
authors.forename: Tolga
authors.initials: T
authors.lastname: Şahin
authors.affiliation: Department of Pharmacognosy, Faculty of Pharmacy, Hacettepe University, 06100 Ankara, Turkey.
authors.forename: A Ahmet
authors.initials: AA
authors.lastname: Başaran
authors.affiliation: Department of Pharmaceutical Toxicology, Faculty of Pharmacy, Hacettepe University, 06100 Ankara, Turkey. Electronic address: nbasaran@hacettepe.edu.tr.
authors.forename: Nurşen
authors.initials: N
authors.lastname: Başaran
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage* / Q000494:pharmacology; D003373:Coumaric Acids / Q000008:administration & dosage* / Q000494:pharmacology; D004249:DNA Damage / Q000187:drug effects*; D005786:Gene Expression Regulation / Q000187:drug effects; D005978:Glutathione / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D007668:Kidney / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008214:Lymphocytes / Q000378:metabolism; D008315:Malondialdehyde / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar; D018805:Sepsis / Q000378:metabolism / Q000473:pathology*; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D000975:Antioxidants; D003373:Coumaric Acids; D008315:Malondialdehyde; C004999:ferulic acid; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005978:Glutathione
keywords: Alkaline single cell electrophoresis; DNA damage; Ferulic acid; Sepsis