title: Overexpression of the Survivin gene in SGC7901 cell resistance to cisplatin.
abstract: The aim of the present study was to investigate the mechanism of SGC7901 cell resistance to cisplatin (CDDP). SGC7901/CDDP cells were established by the long-term continuous exposure of SGC7901 cells to CDDP in stepwise concentration increments. The morphologies of the SGC7901/CDDP and SGC7901 cells were observed by microscopy. The expression levels of Survivin mRNA and protein in the SGC7901/CDDP and SGC7901 cells were examined by reverse transcription polymerase chain reaction and western blotting respectively. The results revealed morphological differences between the SGC7901 and SGC7901/CDDP cells. The expression levels of Survivin mRNA and protein were significantly higher in the SGC7901/CDDP cells than in the SGC7901 cells. Therefore, high expression levels of the Survivin gene may explain SGC7901 cell resistance to CDDP.
authors.affiliation: Department of General Surgery, The Affiliated Nanfang Hospital of Southern Medical University, Guangzhou, Guangdong 510515, P.R. China.
authors.forename: Hanzhang
authors.initials: H
authors.lastname: Dong
authors.affiliation: Department of General Surgery, The Affiliated Beijiao Hospital of Southern Medical University, Shunde, Guangdong 528311, P.R. China.
authors.forename: Gaogao
authors.initials: G
authors.lastname: Liu
authors.affiliation: Department of General Surgery, The Affiliated Beijiao Hospital of Southern Medical University, Shunde, Guangdong 528311, P.R. China.
authors.forename: Biao
authors.initials: B
authors.lastname: Jiang
authors.affiliation: Department of General Surgery, The Affiliated Nanfang Hospital of Southern Medical University, Guangzhou, Guangdong 510515, P.R. China.
authors.forename: Jiubing
authors.initials: J
authors.lastname: Guo
authors.affiliation: Department of General Surgery, The Affiliated Nanfang Hospital of Southern Medical University, Guangzhou, Guangdong 510515, P.R. China.
authors.forename: Guoquan
authors.initials: G
authors.lastname: Tao
authors.affiliation: Department of General Surgery, The Affiliated Beijiao Hospital of Southern Medical University, Shunde, Guangdong 528311, P.R. China.
authors.forename: Wei
authors.initials: W
authors.lastname: Yiu
authors.affiliation: Department of General Surgery, The Affiliated Beijiao Hospital of Southern Medical University, Shunde, Guangdong 528311, P.R. China.
authors.forename: Jingsong
authors.initials: J
authors.lastname: Zhou
authors.affiliation: Department of General Surgery, The Affiliated Nanfang Hospital of Southern Medical University, Guangzhou, Guangdong 510515, P.R. China.
authors.forename: Guoxin
authors.initials: G
authors.lastname: Li
keywords: Survivin; chemotherapy; cisplatin; gastric cancer