title: Ultraselective electrochemiluminescence biosensor based on locked nucleic acid modified toehold-mediated strand displacement reaction and junction-probe.
abstract: Locked nucleic acid (LNA) is applied in toehold-mediated strand displacement reaction (TMSDR) to develop a junction-probe electrochemiluminescence (ECL) biosensor for single-nucleotide polymorphism (SNP) detection in the BRCA1 gene related to breast cancer. More than 65-fold signal difference can be observed with perfectly matched target sequence to single-base mismatched sequence under the same conditions, indicating good selectivity of the ECL biosensor.
authors.affiliation: Department of Pharmaceutical Analysis, Faculty of Pharmacy, Fujian Medical University, Fuzhou, Fujian 350108, P R China. cjh_huaxue@126.com.
authors.forename: Xi
authors.initials: X
authors.lastname: Zhang
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.forename: Dongzhi
authors.initials: D
authors.lastname: Wu
authors.forename: Zhijing
authors.initials: Z
authors.lastname: Liu
authors.forename: Shuxian
authors.initials: S
authors.lastname: Cai
authors.forename: Mei
authors.initials: M
authors.lastname: Chen
authors.forename: Yanping
authors.initials: Y
authors.lastname: Zhao
authors.forename: Chunyan
authors.initials: C
authors.lastname: Li
authors.forename: Huanghao
authors.initials: H
authors.lastname: Yang
authors.forename: Jinghua
authors.initials: J
authors.lastname: Chen
mesh_terms: D019313:BRCA1 Protein / Q000302:isolation & purification*; D001483:Base Sequence; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D004247:DNA / Q000235:genetics; D055664:Electrochemical Techniques / Q000295:instrumentation* / Q000379:methods; D006801:Humans; D049449:Luminescence; D009841:Oligonucleotides / Q000737:chemistry*; D017354:Point Mutation
chemical_list: D019313:BRCA1 Protein; C492913:BRCA1 protein, human; D009841:Oligonucleotides; C477371:locked nucleic acid; D004247:DNA