title: Sulfitobacter pseudonitzschiae sp. nov., isolated from the toxic marine diatom Pseudo-nitzschia multiseries.
abstract: A taxonomic study was carried out on bacterial strain H3(T), which was isolated from the toxic marine diatom Pseudo-nitzschia multiseries. Cells of strain H3(T) were Gram-stain-negative, rod-shaped, non-motile and capable of reducing nitrate to nitrite, but not denitrification. Growth was observed at NaCl concentrations of 1-9%, pH 6-12 and 10-37 °C. It was unable to degrade aesculin or gelatin. The dominant fatty acids (>10 %) were C18:1ω7c/ω6c (summed feature 8) and C16:0. The respiratory ubiquinone was Q10. The major lipids were phosphatidylethanolamine, phosphatidylglycerol, an aminolipid and one unknown lipid, and the minor lipids were two phospholipids and three unknown lipids. The G+C content of the chromosomal DNA was 61.7 mol%. 16S rRNA gene sequence comparison showed that strain H3(T) was related most closely to Sulfitobacter donghicola DSW-25(T) (97.3% similarity) and levels of similarity with other species of the genus Sulfitobacter were 95.1-96.9%. The mean (± sd) DNA-DNA hybridization value between strain H3(T) and Sulfitobacter donghicola DSW-25(T) was 18.0 ± 2.25%. The average nucleotide identity between strain H3(T) and Sulfitobacter donghicola DSW-25(T) was 70.45%. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain H3(T) formed a separate clade close to the genus Sulfitobacter and was distinguishable from phylogenetically related species by differences in several phenotypic properties. On the basis of the phenotypic and phylogenetic data, strain H3(T) represents a novel species of the genus Sulfitobacter, for which the name Sulfitobacter pseudonitzschiae is proposed (type strain H3(T) =DSM 26824(T) =MCCC 1A00686(T)).
authors.affiliation: Engineering Research Center of Marine Biological Resource Comprehensive Utilization, the Third Institute of Oceanography (TIO), State Oceanic Administration (SOA), Xiamen 361005, PR China.|School of Life Sciences, Xiamen University, Xiamen 361102, PR China.
authors.forename: Zhuan
authors.initials: Z
authors.lastname: Hong
authors.affiliation: Key Laboratory of Marine Biogenetic Resources, the Third Institute of Oceanography (TIO), State Oceanic Administration (SOA), Xiamen 361005, PR China.
authors.forename: Qiliang
authors.initials: Q
authors.lastname: Lai
authors.affiliation: Fujian Institute of Oceanology, Xiamen 361012, PR China.
authors.forename: Qiaoqi
authors.initials: Q
authors.lastname: Luo
authors.affiliation: Engineering Research Center of Marine Biological Resource Comprehensive Utilization, the Third Institute of Oceanography (TIO), State Oceanic Administration (SOA), Xiamen 361005, PR China.
authors.forename: Simeng
authors.initials: S
authors.lastname: Jiang
authors.affiliation: Engineering Research Center of Marine Biological Resource Comprehensive Utilization, the Third Institute of Oceanography (TIO), State Oceanic Administration (SOA), Xiamen 361005, PR China.
authors.forename: Ruilin
authors.initials: R
authors.lastname: Zhu
authors.affiliation: School of Life Sciences, Xiamen University, Xiamen 361102, PR China.
authors.forename: Junrong
authors.initials: J
authors.lastname: Liang
authors.affiliation: School of Life Sciences, Xiamen University, Xiamen 361102, PR China.
authors.forename: Yahui
authors.initials: Y
authors.lastname: Gao
mesh_terms: D001267:Atlantic Ocean; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D017377:Diatoms / Q000382:microbiology*; D005227:Fatty Acids / Q000737:chemistry; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D041902:Rhodobacteraceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D012623:Seawater / Q000382:microbiology; D017422:Sequence Analysis, DNA; D014451:Ubiquinone / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D014451:Ubiquinone; C025204:Ubiquinone Q2