title: Development of the large-scale oligonucleotide chip for the diagnosis of plant viruses and its practical use.
abstract: A large-scale oligonucleotide (LSON) chip was developed for the detection of the plant viruses with known genetic information. The LSON chip contains two sets of 3,978 probes for 538 species of targets including plant viruses, satellite RNAs and viroids. A hundred forty thousand probes, consisting of isolate-, species- and genus-specific probes respectively, are designed from 20,000 of independent nucleotide sequence of plant viruses. Based on the economic importance, the amount of genome information, and the number of strains and/or isolates, one to fifty-one probes for each target virus are selected and spotted on the chip. The standard and field samples for the analysis of the LSON chip have been prepared and tested by RT-PCR. The probe's specific and/or nonspecific reaction patterns by LSON chip allow us to diagnose the unidentified viruses. Thus, the LSON chip in this study could be highly useful for the detection of unexpected plant viruses, the monitoring of emerging viruses and the fluctuation of the population of major viruses in each plant.
authors.affiliation: Institute of Plant Medicine, Kyungpook National University, Daegu 702-701, Korea.
authors.forename: Moon
authors.initials: M
authors.lastname: Nam
authors.affiliation: Agricultural Microbiology Division, National Academy of Agricultural Science, RDA, Suwon 441-707, Korea.
authors.forename: Jeong-Seon
authors.initials: JS
authors.lastname: Kim
authors.affiliation: Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 305-806, Korea ; Biosystems and Bioengineering Program, University of Science and Technology, Daejeon 305-350, Korea.
authors.forename: Seungmo
authors.initials: S
authors.lastname: Lim
authors.affiliation: School of Applied Biosciences, Kyungpook National University, Daegu 702-701, Korea.
authors.forename: Chung Youl
authors.initials: CY
authors.lastname: Park
authors.affiliation: Department of Applied Biology, Chungnam National University, Daejeon 305-764, Korea.
authors.forename: Jeong-Gyu
authors.initials: JG
authors.lastname: Kim
authors.affiliation: Crop Protection Division, National Academy of Agricultural Science, RDA, Suwon 441-707, Korea.
authors.forename: Hong-Soo
authors.initials: HS
authors.lastname: Choi
authors.affiliation: Department of Applied Biology, Chungnam National University, Daejeon 305-764, Korea.
authors.forename: Hyoun-Sub
authors.initials: HS
authors.lastname: Lim
authors.affiliation: Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 305-806, Korea ; Biosystems and Bioengineering Program, University of Science and Technology, Daejeon 305-350, Korea.
authors.forename: Jae Sun
authors.initials: JS
authors.lastname: Moon
authors.affiliation: Institute of Plant Medicine, Kyungpook National University, Daegu 702-701, Korea ; School of Applied Biosciences, Kyungpook National University, Daegu 702-701, Korea.
authors.forename: Su-Heon
authors.initials: SH
authors.lastname: Lee
keywords: LSON chip; Large-scale oligonucleotide chip; oligo chip; plant virus; virus diagnosis