title: Liquid base cytology in evaluation of thyroid nodules.
abstract: BACKGROUND
  Palpable thyroid nodules are present in 4-7% of general population and Fine Needle Aspiration (FNA) is now accepted by endocrinologists and thyroid surgeons as a safe, simple and cost effective procedure for evaluating a thyroid nodule. The obtained sample can be spread directly on slides, processed as cell block preparations or prepared as liquid base smears. Liquid base method has been recently accepted due to its shorter preparation time and better preservation of nuclear details. The aim of this study is to compare the diagnostic results of two commonly used methods: Liquid Base Preparation and Cell Block Preparation in evaluation of thyroid nodules.
  
  
  METHODS
  The samples were taken from 100 patients with a solitary nodule or a prominent nodule on a multinodular goiter background (excluding hot nodules). The obtained samples were used to prepare conventional smears (CS), Cell Block Preparations (CBP) and Liquid Base Preparations (LBP). The slides were studied by two pathologists, considering the following parameters: Cellularity, Colloid, Lymphocytes/Plasma cells and Macrophages.
  
  
  RESULTS
  87% of cases revealed informative results in LBP method while in the same group of patients only 69% of samples were informative after processing by CBP method. Sensitivity and specificity of both methods compared with the conventional smears and with each other and it is concluded that LBP is a reliable method for evaluating of a thyroid nodule. Other studies also show the same results.
  
  
  CONCLUSIONS
  The liquid base method should be trusted due to its easier procedure, cleaner slide background, its higher specificity and higher diagnostic yields. It can be used instead of CBP and in association with CS to increase the accuracy of evaluation of thyroid nodules.
authors.affiliation: Genetics Research Center-University of Social Welfare and Rehabilitation Sciences, Koudakyar st.-Daneshjoo blv., Tehran, (1985713834) Iran.
authors.forename: Elahe
authors.initials: E
authors.lastname: Keyhani
authors.affiliation: Endocrinology and Metabolism Research Center, Tehran University of Medical Sciences, Tehran, (1411413137) Iran.
authors.forename: Sasan A
authors.initials: SA
authors.lastname: Sharghi
authors.affiliation: Sepid Pathobiology Laboratory, No.831-North Taleghani Blv., Karaj, (3155783618) Iran.
authors.forename: Rana
authors.initials: R
authors.lastname: Amini
authors.affiliation: Iran University of Medical Sciences-Hemmat Highway, Tehran, (1449614535) Iran.
authors.forename: Sina A
authors.initials: SA
authors.lastname: Sharghi
authors.affiliation: Social Department of Health Research Center, Department of Biostatistics, University of Social Welfare and Rehabilitation Sciences, Tehran, (1985713834) Iran.
authors.forename: Masoud
authors.initials: M
authors.lastname: Karimlou
authors.affiliation: Genetics Research Center-University of Social Welfare and Rehabilitation Sciences, Koudakyar st.-Daneshjoo blv., Tehran, (1985713834) Iran.
authors.forename: Fatemeh A
authors.initials: FA
authors.lastname: Moghaddam
authors.affiliation: Endocrinology and Metabolism Research Center, Tehran University of Medical Sciences, Tehran, (1411413137) Iran.
authors.forename: Bagher
authors.initials: B
authors.lastname: Larijani
keywords: Cell block preparation; Conventional smears; Liquid base preparation; Thyroid fine needle aspiration