title: Effect of oxytocin on serum biochemistry, liver enzymes, and metabolic hormones in lactating Nili Ravi buffaloes.
abstract: Studies reporting the effects of oxytocin on the health of lactating animals are lacking and still no such data is available on Nili Ravi buffalo, the most prominent Asian buffalo breed. The present study was conducted to investigate the effect of oxytocin on physiological and metabolic parameters of lactating Nili Ravi buffaloes. Healthy lactating buffaloes (n = 40) of recent calving were selected from a commercial dairy farm situated in the peri-urban area of district Faisalabad, Pakistan. These buffaloes were randomly allocated to two equal groups viz experimental and control, comprising 20 animals each. Twice-a-day (morning and evening) milking practice was followed. The experimental and control buffaloes were administered subcutaneously with 3 mL of oxytocin (10 IU/mL) and normal saline respectively, prior to each milking. Serum biochemical profile including glucose, total cholesterol (tChol), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C), triglycerides (TG), total proteins (TP), C-reactive protein (CRP), liver enzymes aspartate transaminase (AST), alanine transaminase (ALT), and metabolic hormones triiodothyronine (T₃) and thyroxine (T₄) were studied. Results revealed significantly higher (P ≤ 0.01) levels of glucose, total cholesterol, LDL-C, triglycerides, total proteins, and C-reactive protein in experimental (oxytocin-injected) lactating buffaloes compared to control group. Liver enzymes AST and ALT as well as serum T₄ concentration was significantly higher (P ≤ 0.01) in oxytocin-injected lactating buffaloes as compared to control animals. It was concluded that oxytocin had the key role in increasing the metabolic parameters and hormones, resulting in the optimization of production. But, at the same time, it may pose a threat to the animal health.
authors.affiliation: Department of Biosciences, Faculty of Veterinary Sciences, Bahauddin Zakariya University, Multan, Pakistan, drzafariqbaluaf@hotmail.com.
authors.forename: Zafar
authors.initials: Z
authors.lastname: Iqbal
authors.forename: Zia
authors.initials: Z
authors.lastname: ur Rahman
authors.forename: Faqir
authors.initials: F
authors.lastname: Muhammad
authors.forename: Masood
authors.initials: M
authors.lastname: Akhtar
authors.forename: Mian Muhammad
authors.initials: MM
authors.lastname: Awais
authors.forename: Tanweer
authors.initials: T
authors.lastname: Khaliq
authors.forename: Amar
authors.initials: A
authors.lastname: Nasir
authors.forename: Muhammad
authors.initials: M
authors.lastname: Nadeem
authors.forename: Kinza
authors.initials: K
authors.lastname: Khan
authors.forename: Hafiz Muhammad
authors.initials: HM
authors.lastname: Arshad
authors.forename: Muhammad Abdul
authors.initials: MA
authors.lastname: Basit
mesh_terms: D000410:Alanine Transaminase / Q000097:blood*; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood*; D002020:Buffaloes / Q000097:blood / Q000502:physiology*; D002097:C-Reactive Protein / Q000378:metabolism; D002784:Cholesterol / Q000097:blood; D008076:Cholesterol, HDL / Q000097:blood; D008078:Cholesterol, LDL / Q000097:blood; D005260:Female; D006728:Hormones / Q000097:blood; D007774:Lactation / Q000097:blood* / Q000187:drug effects*; D008099:Liver / Q000187:drug effects / Q000201:enzymology; D008892:Milk; D010121:Oxytocin / Q000097:blood*; D010154:Pakistan; D014280:Triglycerides / Q000097:blood
chemical_list: D008076:Cholesterol, HDL; D008078:Cholesterol, LDL; D006728:Hormones; D014280:Triglycerides; D010121:Oxytocin; D002097:C-Reactive Protein; D002784:Cholesterol; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase