title: Visualization of nitroxyl (HNO) in vivo via a lysosome-targetable near-infrared fluorescent probe.
abstract: We have presented a near-infrared fluorescent probe Lyso-JN for the detection of nitroxyl (HNO) in cells and in vivo. Lyso-JN is comprised of three moieties: an Aza-BODIPY fluorophore, a HNO-response modulator, diphenylphosphino-benzoyl, and a lysosomal locator, alkylmorpholine. The detection mechanism is based on aza-ylide intramolecular ester aminolysis reaction with HNO. The probe holds the ability to capture lysosomal HNO in RAW 264.7 cells, and it is also successfully employed to visualize HNO in mice.
authors.affiliation: The Key Laboratory of Life-Organic Analysis, College of Chemistry and Chemical Engineering, Qufu Normal University, Qufu 273165, China.
authors.forename: Xiaotong
authors.initials: X
authors.lastname: Jing
authors.forename: Fabiao
authors.initials: F
authors.lastname: Yu
authors.forename: Lingxin
authors.initials: L
authors.lastname: Chen
mesh_terms: D000818:Animals; D002460:Cell Line; D005456:Fluorescent Dyes / Q000008:administration & dosage / Q000378:metabolism* / Q000493:pharmacokinetics*; D008247:Lysosomes / Q000737:chemistry / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D015394:Molecular Structure; D009589:Nitrogen Oxides / Q000032:analysis*
chemical_list: D005456:Fluorescent Dyes; D009589:Nitrogen Oxides; C039900:nitroxyl