title: Differential expression of VEGF and IL-1alpha after photodynamic treatment in combination with doxorubicin or taxotere.
abstract: OBJECTIVE
  To show the impact of chemotherapeutic drugs doxorubicin and taxotere on the molecular pattern of cell response to photodynamic treatment (PDT).
  
  
  METHODS
  Human squamous cell carcinoma cells A-431 were studied. Apoptosis was investigated by recording caspase-3 activity. Expression of IL-1alpha and VEGF on mRNA and protein levels was measured by qPCR and ELISA.
  
  
  RESULTS
  PDT in combination with either doxorubicin or taxotere was found to be more cytotoxic in comparison to either single-treatment. The expression of IL-1alpha and VEGF was up-regulated in PDT-treated cells, either alone or in combination with doxorubicin or taxotere. Addition of doxorubicin to the cytokine induction after PDT was not detected, however, taxotere promoted significant over-expression of IL-1alpha and VEGF on the protein level.
  
  
  CONCLUSIONS
  Contribution of chemotherapeutic drugs to IL-1 alpha and VEGF release from cells which received dual treatment involving PDT could be significantly different, despite the same level of cytotoxicity.
authors.affiliation: Department of Biochemistry and Molecular Biology, Vilnius University, Vilnius, Lithuania.
authors.forename: Daiva
authors.initials: D
authors.lastname: Dabkeviciene
authors.affiliation: Department of Biochemistry and Molecular Biology, Vilnius University, Vilnius, Lithuania.
authors.forename: Ausra
authors.initials: A
authors.lastname: Sasnauskiene
authors.affiliation: Department of Biochemistry and Molecular Biology, Vilnius University, Vilnius, Lithuania Department of Evolutionary and Environmental Biology, University of Haifa, Haifa, Israel.
authors.forename: Eva
authors.initials: E
authors.lastname: Leman
authors.affiliation: Department of Biochemistry and Molecular Biology, Vilnius University, Vilnius, Lithuania.
authors.forename: Raimonda
authors.initials: R
authors.lastname: Kvietkauskaite
authors.affiliation: Department of Biochemistry and Molecular Biology, Vilnius University, Vilnius, Lithuania vida.kirveliene@gf.vu.lt.
authors.forename: Vida
authors.initials: V
authors.lastname: Kirveliene
mesh_terms: D000903:Antibiotics, Antineoplastic / Q000494:pharmacology / Q000633:toxicity; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects / Q000235:genetics; D000077143:Docetaxel; D004305:Dose-Response Relationship, Drug; D004317:Doxorubicin / Q000494:pharmacology* / Q000633:toxicity; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects*; D006801:Humans; D053582:Interleukin-1alpha / Q000235:genetics*; D010778:Photochemotherapy; D043823:Taxoids / Q000494:pharmacology* / Q000633:toxicity; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics*
chemical_list: D000903:Antibiotics, Antineoplastic; D053582:Interleukin-1alpha; D043823:Taxoids; D042461:Vascular Endothelial Growth Factor A; D000077143:Docetaxel; D004317:Doxorubicin
keywords: IL-1alpha; Photodynamic therapy; VEGF; doxorubicin; mTHPC; taxotere