title: Karyotype stability of human umbilical cord-derived mesenchymal stem cells duringin vitroculture.
abstract: The aim of this study was to investigate whether the chromosomes of human umbilical cord-derived mesenchymal stem cells (hUCMSCs) change followingin vitroculture for several generations. In the present study, umbilical cords from two healthy infants following cesarean delivery were collected aseptically and hUCMSCs were isolated by digestion with collagenase and trypsin, and then culturedin vitro. hUCMSCs with fibroblastic morphology were presented from the human umbilical cord tissue after 7 days of adherent culture. When cultured for 6 passagesin vitro, the hUCMSCs maintained a stable spindle-shaped morphology. Cells reached the logarithmic growth phase after 3-4 days of culture. In addition, CD13, CD29, CD44, CD90 and CD105 were highly expressed in generations P3-P6. The expression of CD31, CD34, CD45 and HLA-DR was negative. Furthermore, karyotype analysis revealed a normal diploid karyotype with 46 chromosomes and no abnormal changes were found in chromosome structure. These findings suggest that when cultured for 6 passagesin vitro, hUCMSCs maintain a stable immunophenotype and chromosome structure, which provides an experimental basis for the safety of hUCMSC cytotherapy.
authors.affiliation: Department of Cardiology, Taizhou People's Hospital, Taizhou, Jiangsu 225300, P.R. China.
authors.forename: Zhong-Bao
authors.initials: ZB
authors.lastname: Ruan
authors.affiliation: Department of Cardiology, Taizhou People's Hospital, Taizhou, Jiangsu 225300, P.R. China.
authors.forename: Li
authors.initials: L
authors.lastname: Zhu
authors.affiliation: Department of Cardiology, Taizhou People's Hospital, Taizhou, Jiangsu 225300, P.R. China.
authors.forename: Yi-Gang
authors.initials: YG
authors.lastname: Yin
authors.affiliation: Department of Cardiology, Taizhou People's Hospital, Taizhou, Jiangsu 225300, P.R. China.
authors.forename: Ge-Cai
authors.initials: GC
authors.lastname: Chen
keywords: human umbilical cord-derived mesenchymal stem cells; karyotyping; serial passage