title: Domain 2 of a Kazal serine proteinase inhibitor SPIPm2 from Penaeus monodon possesses antiviral activity against WSSV.
abstract: A 5-domain Kazal type serine proteinase inhibitor SPIPm2 from Penaeus monodon is involved in innate immune defense against white spot syndrome virus (WSSV). To test which domains were involved, the 5 domains of SPIPm2 were over-expressed and tested against WSSV infection. By using hemocyte primary cell culture treated with each recombinant SPIPm2 domain along with WSSV, the expression of WSSV early genes ie1, WSV477 and late gene VP28 were substantially reduced as compared to other domains when the recombinant domain 2, rSPIPm2D2, was used. Injecting the WSSV along with rSPIPm2D2 but not with other domains caused delay in mortality rate of the infected shrimp. The results indicate that the SPIPm2D2 possesses strong antiviral activity and, hence, contributes predominantly to the antiviral activity of SPIPm2.
authors.affiliation: Center of Excellence for Molecular Biology and Genomics of Shrimp, Department of Biochemistry, Faculty of Science, Chulalongkorn University, Phyathai Road, Pathumwan, Bangkok 10330, Thailand.
authors.forename: Suwattana
authors.initials: S
authors.lastname: Visetnan
authors.affiliation: Department of Chemistry, Faculty of Science, Srinakharinwirot University, Bangkok 10110, Thailand. Electronic address: dsuchao@gmail.com.
authors.forename: Suchao
authors.initials: S
authors.lastname: Donpudsa
authors.affiliation: Center of Excellence for Molecular Biology and Genomics of Shrimp, Department of Biochemistry, Faculty of Science, Chulalongkorn University, Phyathai Road, Pathumwan, Bangkok 10330, Thailand; National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), Pathum Thani 10120, Thailand.
authors.forename: Premruethai
authors.initials: P
authors.lastname: Supungul
authors.affiliation: Center of Excellence for Molecular Biology and Genomics of Shrimp, Department of Biochemistry, Faculty of Science, Chulalongkorn University, Phyathai Road, Pathumwan, Bangkok 10330, Thailand.
authors.forename: Anchalee
authors.initials: A
authors.lastname: Tassanakajon
authors.affiliation: Center of Excellence for Molecular Biology and Genomics of Shrimp, Department of Biochemistry, Faculty of Science, Chulalongkorn University, Phyathai Road, Pathumwan, Bangkok 10330, Thailand. Electronic address: kpvr2@yahoo.com.
authors.forename: Vichien
authors.initials: V
authors.lastname: Rimphanitchayakit
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002478:Cells, Cultured; D017931:DNA Primers / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D015967:Gene Expression Regulation, Viral / Q000187:drug effects*; D006434:Hemocytes / Q000378:metabolism; D007113:Immunity, Innate / Q000235:genetics / Q000276:immunology*; D008969:Molecular Sequence Data; D033561:Penaeidae / Q000235:genetics / Q000276:immunology* / Q000821:virology*; D017434:Protein Structure, Tertiary; D015842:Serine Proteinase Inhibitors / Q000235:genetics / Q000494:pharmacology*; D046848:White spot syndrome virus 1 / Q000187:drug effects*
chemical_list: D017931:DNA Primers; D015842:Serine Proteinase Inhibitors
keywords: Kazal domain; Penaeus monodon; Serine proteinase inhibitor; Shrimp survival; WSSV