title: A multiplexed droplet digital PCR assay performs better than qPCR on inhibition prone samples.
abstract: We demonstrate the development of a multiplex droplet digital PCR assay for human cytomegalovirus (CMV), human adenovirus species F, and an internal plasmid control that may be useful for PCR inhibition-prone clinical samples. This assay performs better on inhibition-prone stool samples than a quantitative PCR assay for CMV and is the first published clinical virology droplet digital PCR assay to incorporate an internal control.
authors.affiliation: Molecular Virology Laboratory, Department of Laboratory Medicine, University of Washington, Seattle, WA.
authors.forename: Ruth Hall
authors.initials: RH
authors.lastname: Sedlak
authors.affiliation: Molecular Virology Laboratory, Department of Laboratory Medicine, University of Washington, Seattle, WA.
authors.forename: Jane
authors.initials: J
authors.lastname: Kuypers
authors.affiliation: Molecular Virology Laboratory, Department of Laboratory Medicine, University of Washington, Seattle, WA; Vaccine Infectious Disease Division, Fred Hutchinson Cancer Research Center, Seattle, WA. Electronic address: kjerome@fhcrc.org.
authors.forename: Keith R
authors.initials: KR
authors.lastname: Jerome
mesh_terms: D000260:Adenoviruses, Human / Q000235:genetics*; D003587:Cytomegalovirus / Q000235:genetics*; D003586:Cytomegalovirus Infections / Q000821:virology; D005243:Feces / Q000821:virology*; D006801:Humans; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods*; D060888:Real-Time Polymerase Chain Reaction
keywords: Digital PCR; Inhibition; Quantitative PCR; Stool