title: Recent bioanalytical methods for quantification of third-generation cephalosporins using HPLC and LC-MS(/MS) and their applications in pharmacokinetic studies.
abstract: Third-generation cephalosporins are semi-synthetic antibiotics with enhanced activity against Gram-negative organisms. The cephalosporins in biological samples are mostly determined using high-performance liquid chromatography (HPLC) and HPLC-mass spectrometry (LC-MS) or tandem mass spectrometry (LC-MS/MS). In recent years, numerous bioanalytical methods have been developed to improve the sensitivity and specificity of cephalosporin quantification using the powerful LC-MS/MS systems that are common in research laboratories. This review article presents recently developed bioanalytical methods by HPLC or LC-MS(/MS) for 12 third-generation cephalosporins, including five oral third-generation cephalosporins, and further discusses their application in pharmacokinetic studies of animals and humans.
authors.affiliation: College of Pharmacy, Seoul National University, Seoul, Republic of Korea.
authors.forename: Hyo-Eon
authors.initials: HE
authors.lastname: Jin
authors.forename: Su-Eon
authors.initials: SE
authors.lastname: Jin
authors.forename: Han-Joo
authors.initials: HJ
authors.lastname: Maeng
mesh_terms: D000818:Animals; D002511:Cephalosporins / Q000032:analysis* / Q000737:chemistry / Q000493:pharmacokinetics*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D002511:Cephalosporins
keywords: HPLC; LC-MS; LC-MS/MS; bioanalytical methods; pharmacokinetics; third-generation cephalosporins