title: Effect of fibroblast growth factor and enamel matrix derivative treatment on root resorption after delayed replantation.
abstract: BACKGROUND
  Periodontal ligament (PDL) healing and long term prognosis of replanted avulsed teeth should rely on several factors including length of extra-oral dry time and type of the storage medium. The status of periodontal ligament is critical for the healing of replanted teeth. Different substances have been used for root surface treatment to promote formation of PDL and increase the survival of avulsed teeth submitted to replantation.
  
  
  OBJECTIVE
  The purpose of this study was to assess the effect of recombinant basic fibroblast growth factor 2 (bFGF) and enamel matrix derivative (EMD) on root resorption after delayed replantation.
  
  
  METHODS
  18 freshly extracted single-rooted incisor and premolar teeth were extracted from the beagle dogs and immersed in whole bovine milk for 45 and 60 min (n = 3 each). Following storage period, sockets washed and teeth were treated with bFGF and EMD and replanted into the sockets. After 8 weeks, dogs were sacrificed, specimens processed to 4-μm thick serial sections for histopathologic examination and morphometric assessments. Thus, the proportions of the roots that exhibited signs of surface resorption, inflammatory resorption, and replacement resorption, that is, ankylosis and normal PDL were noted.
  
  
  RESULTS
  The percentage of root resorption was in the following order: EMD>milk>bFGF for 45 min and milk>EMD>bFGF for 60 min. For all groups, teeth stored 60 min showed significantly higher incidence of PDL resorption than those stored for 45 min (P < 0.01). The highest incidence of replacement resorption was observed in teeth treated with EMD for 60 min. After 8 weeks, the least resorption was found in bFGF-treated group (P < 0.01).
  
  
  CONCLUSIONS
  The findings of this study suggest that use of bFGF favored the formation of new periodontal ligament; prevent ankylosis and resorption process following delayed replantation of teeth while EMD shows replacement resorption, which may turn to ankylosis.
authors.affiliation: Department of Pediatric Dentistry, Nihon University School of Dentistry at Matsudo, Chiba, Japan; Department of Pedodontics, Istanbul University Faculty of Dentistry, Istanbul, Turkey.
authors.forename: Elif Bahar
authors.initials: EB
authors.lastname: Tuna
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Arai
authors.forename: Merva Soluk
authors.initials: MS
authors.lastname: Tekkesin
authors.forename: Figen
authors.initials: F
authors.lastname: Seymen
authors.forename: Koray
authors.initials: K
authors.lastname: Gencay
authors.forename: Noboru
authors.initials: N
authors.lastname: Kuboyama
authors.forename: Takahide
authors.initials: T
authors.lastname: Maeda
mesh_terms: D000818:Animals; D003746:Dental Enamel Proteins / Q000494:pharmacology*; D004285:Dogs; D005346:Fibroblast Growth Factors / Q000494:pharmacology*; D008892:Milk; D012391:Root Resorption / Q000517:prevention & control*; D013997:Time Factors; D020254:Tooth Ankylosis / Q000517:prevention & control*; D014081:Tooth Extraction; D014090:Tooth Replantation; D055114:X-Ray Microtomography
chemical_list: D003746:Dental Enamel Proteins; C109069:enamel matrix proteins; D005346:Fibroblast Growth Factors
keywords: Dog; Enamel Matrix Derivative; Fibroblast Growth Factor-basic; Periodontal Ligament; Tooth Replantation