title: Cryptic speciation in the white-shouldered antshrike (Thamnophilus aethiops, Aves - Thamnophilidae): the tale of a transcontinental radiation across rivers in lowland Amazonia and the northeastern Atlantic Forest.
abstract: The growing knowledge on paleogeography and the recent applications of molecular biology and phylogeography to the study of the Amazonian biota have provided a framework for testing competing hypotheses of biotic diversification in this region. Here, we reconstruct the spatio-temporal context of diversification of a widespread understory polytypic Amazonian bird species (Thamnophilus aethiops) and contrast it with different hypotheses of diversification and the taxonomy currently practiced in the group. Sequences of mtDNA (cytochrome b and ND2) and nuclear (β-fibrinogen introns 5 and 7 and the Z-liked Musk4) genes, adding up to 4093bp of 89 individuals covering the Amazonian, Andean, and Atlantic Forest populations of T. aethiops were analyzed. Phylogenetic and population genetics analyses revealed ten reciprocally monophyletic and genetically isolated or nearly-isolated lineages in T. aethiops, highlighting several inconsistencies between taxonomy and evolutionary history in this group. Our data suggest that the diversification of T. aethiops started in the Andean highlands, and then proceeded into the Amazonian lowlands probably after the consolidation of the modern Amazonian drainage. The main cladogenetic events in T. aethiops may be related to the formation and structuring of large Amazonian rivers during the Late Miocene-Early Pleistocene, coinciding with the dates proposed for other lineages of Amazonian organisms. Population genetics data do not support climatic fluctuations as a major source of diversification in T. aethiops. Even though not entirely concordant with paleobiogeographic models derived from phylogenies of other vertebrate lineages, our results support a prominent role for rivers as major drivers of diversification in Amazonia, while underscoring that different diversification scenarios are probably related to the distinct evolutionary origins of groups being compared.
authors.affiliation: Curso de Pós-graduação em Zoologia, Universidade Federal do Pará/Museu Paraense Emílio Goeldi, Caixa Postal 399, CEP 66040-170 Belém, PA, Brazil.
authors.forename: Gregory
authors.initials: G
authors.lastname: Thom
authors.affiliation: Coordenação de Zoologia, Museu Paraense Emílio Goeldi, Caixa Postal 399, CEP 66040-170 Belém, PA, Brazil. Electronic address: aleixo@museu-goeldi.br.
authors.forename: Alexandre
authors.initials: A
authors.lastname: Aleixo
mesh_terms: D000818:Animals; D001499:Bayes Theorem; D004272:DNA, Mitochondrial / Q000235:genetics; D065928:Forests; D051456:Gene Flow; D049810:Genetic Speciation; D005828:Genetics, Population; D007438:Introns; D016013:Likelihood Functions; D008957:Models, Genetic; D046109:Passeriformes / Q000145:classification* / Q000235:genetics; D010802:Phylogeny; D058974:Phylogeography; D045483:Rivers; D017422:Sequence Analysis, DNA; D013020:South America
chemical_list: D004272:DNA, Mitochondrial
keywords: Diversification hypotheses; Historical biogeography; Phylogeography; Population genetics; Refuges; Taxonomy