title: Tetracycline-regulated mouse models of cancer.
abstract: Genetically engineered mouse models (GEMMs) have proven essential to the study of mammalian gene function in both development and disease. However, traditional constitutive transgenic mouse model systems are limited by the temporal and spatial characteristics of the experimental promoter used to drive transgene expression. To address this limitation, considerable effort has been dedicated to developing conditional and inducible mouse model systems. Although a number of approaches to generating inducible GEMMs have been pursued, several have been restricted by toxic or undesired physiological side effects of the compounds used to activate gene expression. The development of tetracycline (tet)-dependent regulatory systems has allowed for circumvention of these issues resulting in the widespread adoption of these systems as an invaluable tool for modeling the complex nature of cancer progression.
authors.affiliation: Department of Cancer Biology, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104; Abramson Family Cancer Research Institute, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104.
authors.forename: Elizabeth S
authors.initials: ES
authors.lastname: Yeh
authors.affiliation: Department of Cancer Biology, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104; Abramson Family Cancer Research Institute, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104.
authors.forename: Ann
authors.initials: A
authors.lastname: Vernon-Grey
authors.affiliation: Department of Cancer Biology, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104; Abramson Family Cancer Research Institute, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104.
authors.forename: Heather
authors.initials: H
authors.lastname: Martin
authors.affiliation: Department of Cancer Biology, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104; Department of Cell and Developmental Biology, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104; Department of Medicine, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104; Abramson Family Cancer Research Institute, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104.
authors.forename: Lewis A
authors.initials: LA
authors.lastname: Chodosh
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D005786:Gene Expression Regulation / Q000235:genetics*; D006801:Humans; D051379:Mice; D009369:Neoplasms / Q000235:genetics*; D009875:Operator Regions, Genetic / Q000235:genetics; D013752:Tetracycline / Q000096:biosynthesis*; D015534:Trans-Activators / Q000096:biosynthesis* / Q000235:genetics; D019076:Transgenes
chemical_list: D015534:Trans-Activators; D013752:Tetracycline