title: Polymorphisms of DNA repair genes and lung cancer in chromium exposure.
abstract: Chromium is a well known carcinogen involved in the lung cancer development. Polymorphism of some of the DNA repair genes may be associated with elevated risk of cancerous transformation. In the present study, we investigated the polymorphisms of the following selected members of the base and nucleotide excision repair genes: XPC (Lys939Gln), XPD (Lys751Gln), XRCC1(Arg399Gln), and hOGG1(Ser326Ser), and the risk they present toward the development of lung cancer, with emphasis on the effect of chromium exposure. We analyzed 119 individuals; 50 patients exposed to chromium with diagnosed lung cancer and 69 healthy controls. Genotypes were determined by a PCR-RFLP method. We found a significantly increased risk of lung cancer development in XPD genotype Lys/Gln (OR=1.94; 95% CI=1.10-3.43; p=0.015) and in the gene combinations: XPD Lys/Gln+XPC Lys/Gln (OR=6.5; 95% CI=1.53-27.49; p=0.009) and XPD Lys/Gln+XPC Gln/Gln(OR=5.2; 95% CI=1.07-25.32; p=0.04). In conclusion, gene polymorphisms in the DNA repair genes may underscore the risk of lung cancer development in the chromium-exposed individuals.
authors.affiliation: Department of Medical Biochemistry, Jessenius Faculty of Medicine in Martin, Comenius University in Bratislava, 4 Malá Hora St., 036 01, Martin, Slovakia.
authors.forename: M
authors.initials: M
authors.lastname: Sarlinova
authors.forename: L
authors.initials: L
authors.lastname: Majerova
authors.forename: T
authors.initials: T
authors.lastname: Matakova
authors.forename: L
authors.initials: L
authors.lastname: Musak
authors.forename: P
authors.initials: P
authors.lastname: Slovakova
authors.forename: M
authors.initials: M
authors.lastname: Skereňová
authors.forename: E
authors.initials: E
authors.lastname: Kavcová
authors.forename: E
authors.initials: E
authors.lastname: Halašová
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002857:Chromium / Q000633:toxicity*; D004260:DNA Repair; D004268:DNA-Binding Proteins / Q000235:genetics; D004781:Environmental Exposure; D005260:Female; D020022:Genetic Predisposition to Disease; D006801:Humans; D008175:Lung Neoplasms / Q000139:chemically induced* / Q000235:genetics; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic; D000076105:X-ray Repair Cross Complementing Protein 1; D051759:Xeroderma Pigmentosum Group D Protein / Q000235:genetics
chemical_list: D004268:DNA-Binding Proteins; D000076105:X-ray Repair Cross Complementing Protein 1; C000619371:XRCC1 protein, human; D002857:Chromium; C086984:XPC protein, human; D051759:Xeroderma Pigmentosum Group D Protein; C497292:ERCC2 protein, human