title: A profluorescent ratiometric probe for intracellular pH imaging.
abstract: A ratiometric pH probe composed of a fluorescein moiety and an ionic near-infrared-emitting phosphorescent cyclometalated iridium(III) complex bis(6-(benzo[b]thien-2-yl)phenanthridinato)(4-(3-carboxypropyl)-4'-methyl-2,2'-bipyridine)iridium(III) was synthesized. With good cell permeability, the probe demonstrated a linear ratiometric response to the pH variation in the physiological range in HeLa cell assay.
authors.affiliation: Division of Nanobiomedicine, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, 398 Ruoshui Road, Suzhou Industrial Park, Suzhou, Jiangsu 215123, PR China; University of Chinese Academy of Sciences, 19A Yuquan Road, Beijing 100049, PR China; Institute of Chemistry, Chinese Academy of Sciences, 2 Zhongguancun North First Street, Beijing 100190, PR China.
authors.forename: Qingqing
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: Division of Nanobiomedicine, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, 398 Ruoshui Road, Suzhou Industrial Park, Suzhou, Jiangsu 215123, PR China; SunaTech Inc., bioBAY, Suzhou Industrial Park, Suzhou, Jiangsu 215125, PR China. Electronic address: mzhou2007@sinano.ac.cn.
authors.forename: Ming
authors.initials: M
authors.lastname: Zhou
mesh_terms: D015082:2,2'-Dipyridyl / Q000737:chemistry*; D056831:Coordination Complexes / Q000737:chemistry*; D003952:Diagnostic Imaging; D005452:Fluoresceins / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007495:Iridium / Q000737:chemistry*; D049408:Luminescent Agents / Q000737:chemistry*; D008958:Models, Molecular; D015394:Molecular Structure; D009942:Organometallic Compounds / Q000737:chemistry*
chemical_list: D056831:Coordination Complexes; D005452:Fluoresceins; D049408:Luminescent Agents; D009942:Organometallic Compounds; D007495:Iridium; D015082:2,2'-Dipyridyl
keywords: Fluorescein; Intracellular pH; Iridium(III) complex; Near-infrared; Profluorescent; Ratiometric