title: NMR study of non-structural proteins--part II: (1)H, (13)C, (15)N backbone and side-chain resonance assignment of macro domain from Venezuelan equine encephalitis virus (VEEV).
abstract: Macro domains consist of 130-190 amino acid residues and appear to be highly conserved in all kingdoms of life. Intense research on this field has shown that macro domains bind ADP-ribose and other similar molecules, but their exact function still remains intangible. Macro domains are highly conserved in the Alphavirus genus and the Venezuelan equine encephalitis virus (VEEV) is a member of this genus that causes fatal encephalitis to equines and humans. In this study we report the high yield recombinant expression and preliminary solution NMR study of the macro domain of VEEV. An almost complete sequence-specific assignment of its (1)H, (15)N and (13)C resonances was obtained and its secondary structure predicted by TALOS+. The protein shows a unique mixed α/β-fold.
authors.affiliation: Department of Pharmacy, University of Patras, 26504, Patras, Greece.
authors.forename: Garyfallia I
authors.initials: GI
authors.lastname: Makrynitsa
authors.affiliation: Department of Pharmacy, University of Patras, 26504, Patras, Greece.
authors.forename: Dioni
authors.initials: D
authors.lastname: Ntonti
authors.affiliation: Department of Pharmacy, University of Patras, 26504, Patras, Greece.
authors.forename: Konstantinos D
authors.initials: KD
authors.lastname: Marousis
authors.affiliation: Department of Pharmacy, University of Patras, 26504, Patras, Greece.
authors.forename: Aikaterini C
authors.initials: AC
authors.lastname: Tsika
authors.affiliation: AFMB UMR 7257, Aix-Marseille Université, 13288, Marseille, France.|AFMB UMR 7257, CNRS, 13288, Marseille, France.
authors.forename: Julie
authors.initials: J
authors.lastname: Lichière
authors.affiliation: AFMB UMR 7257, Aix-Marseille Université, 13288, Marseille, France.|AFMB UMR 7257, CNRS, 13288, Marseille, France.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Papageorgiou
authors.affiliation: AFMB UMR 7257, Aix-Marseille Université, 13288, Marseille, France.|AFMB UMR 7257, CNRS, 13288, Marseille, France.
authors.forename: Bruno
authors.initials: B
authors.lastname: Coutard
authors.affiliation: Institute of Physiology II, University of Freiburg, 79104, Freiburg, Germany.
authors.forename: Detlef
authors.initials: D
authors.lastname: Bentrop
authors.affiliation: Department of Pharmacy, University of Patras, 26504, Patras, Greece. G.A.Spyroulias@upatras.gr.
authors.forename: Georgios A
authors.initials: GA
authors.lastname: Spyroulias
mesh_terms: D000595:Amino Acid Sequence; D066241:Carbon-13 Magnetic Resonance Spectroscopy; D004666:Encephalitis Virus, Venezuelan Equine / Q000378:metabolism*; D008969:Molecular Sequence Data; D009587:Nitrogen Isotopes; D019906:Nuclear Magnetic Resonance, Biomolecular; D017434:Protein Structure, Tertiary; D066244:Proton Magnetic Resonance Spectroscopy; D016415:Sequence Alignment; D017361:Viral Nonstructural Proteins / Q000737:chemistry*
chemical_list: D009587:Nitrogen Isotopes; D017361:Viral Nonstructural Proteins
keywords: ADP-ribose-binding module; Alphavirus; NMR spectroscopy; Recombinant protein expression; Venezuelan equine encephalitis virus; Viral macro domains