title: Characterization and complete genome sequence of a novel N4-like bacteriophage, pSb-1 infecting Shigella boydii.
abstract: Shigellosis is one of major foodborne pathogens in both developed and developing countries. Although antibiotic therapy is considered an effective treatment for shigellosis, the imprudent use of antibiotics has led to the increase of multiple-antibiotic-resistant Shigella species globally. In this study, we isolated a virulent Podoviridae bacteriophage (phage), pSb-1, that infects Shigella boydii. One-step growth analysis revealed that this phage has a short latent period (15 min) and a large burst size (152.63 PFU/cell), indicating that pSb-1 has good host infectivity and effective lytic activity. The double-stranded DNA genome of pSb-1 is composed of 71,629 bp with a G + C content of 42.74%. The genome encodes 103 putative ORFs, 9 putative promoters, 21 transcriptional terminators, and one tRNA region. Genome sequence analysis of pSb-1 and comparative analysis with the homologous phage EC1-UPM, N4-like phage revealed that there is a high degree of similarity (94%, nucleotide sequence identity) between pSb-1 and EC1-UPM in 73 of the 103 ORFs of pSb-1. The results of this investigation indicate that pSb-1 is a novel virulent N4-like phage infecting S. boydii and that this phage might have potential uses against shigellosis.
authors.affiliation: Institute of Marine Biology, Biotechnology and Aquaculture, Hellenic Centre for Marine Research, Former American Base of Gournes, Heraklion 71003, Crete, Greece; Laboratory of Aquatic Biomedicine, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul 151-742, Republic of Korea.
authors.forename: Jin Woo
authors.initials: JW
authors.lastname: Jun
authors.affiliation: Laboratory of Aquatic Biomedicine, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul 151-742, Republic of Korea.
authors.forename: Sae Kil
authors.initials: SK
authors.lastname: Yun
authors.affiliation: Laboratory of Aquatic Biomedicine, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul 151-742, Republic of Korea.
authors.forename: Hyoun Joong
authors.initials: HJ
authors.lastname: Kim
authors.affiliation: Departments of Rheumatology, Bundang Jesaeng Hospital, Seongnam 255-2, Republic of Korea.
authors.forename: Ji Young
authors.initials: JY
authors.lastname: Chai
authors.affiliation: Laboratory of Aquatic Biomedicine, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul 151-742, Republic of Korea. Electronic address: parksec@snu.ac.kr.
authors.forename: Se Chang
authors.initials: SC
authors.lastname: Park
mesh_terms: D001435:Bacteriophages / Q000235:genetics* / Q000254:growth & development / Q000302:isolation & purification*; D001482:Base Composition; D004267:DNA Viruses / Q000235:genetics / Q000302:isolation & purification; D004279:DNA, Viral / Q000737:chemistry* / Q000235:genetics*; D005814:Genes, Viral; D016679:Genome, Viral; D058977:Molecular Sequence Annotation; D008969:Molecular Sequence Data; D017902:Podoviridae / Q000235:genetics / Q000302:isolation & purification; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D012761:Shigella boydii / Q000821:virology*; D026801:Synteny
chemical_list: D004279:DNA, Viral
keywords: Bacteriophage; Podoviridae; Shigella boydii; Shigellosis; pSb-1