title: The use of liquid chromatography tandem mass spectrometry to detect proteins in saliva from horses with and without systemic inflammation.
abstract: The objective of the study was to assess global expression of proteins in equine saliva using liquid chromatography tandem mass spectrometry (LC-MS/MS). Saliva was obtained from seven horses with and six horses without evidence of systemic inflammatory disease. Tryptic peptides from saliva were analysed by LC-MS/MS. Of 195 unique proteins identified, 57 were detected only in saliva samples from horses with systemic inflammation (in two to six of the seven horses). Among the differentially expressed proteins were several acute phase proteins (APPs) such as serum amyloid A, fibrinogen, haptoglobin, and alpha1-acid glycoprotein. The study is the first to describe detection of inflammatory proteins in horse saliva. The proteins detected were similar to those described in saliva from cattle, small ruminants and pigs. Detection of APPs in horses with systemic inflammation suggests that saliva may be used for non-invasive disease monitoring in horses as in humans, pigs and dogs.
authors.affiliation: Department of Large Animal Sciences, Veterinary School of Medicine, Faculty of Health and Medical Sciences, University of Copenhagen, Hoejbakkegaard Allé 5, DK-2630 Taastrup, Denmark. Electronic address: stj@sund.ku.dk.
authors.forename: Stine
authors.initials: S
authors.lastname: Jacobsen
authors.affiliation: Department of Large Animal Sciences, Veterinary School of Medicine, Faculty of Health and Medical Sciences, University of Copenhagen, Hoejbakkegaard Allé 5, DK-2630 Taastrup, Denmark.
authors.forename: Ditte Marie
authors.initials: DM
authors.lastname: Top Adler
authors.affiliation: Department of Large Animal Sciences, Veterinary School of Medicine, Faculty of Health and Medical Sciences, University of Copenhagen, Hoejbakkegaard Allé 5, DK-2630 Taastrup, Denmark; Department of Molecular Biology and Genetics, Aarhus University, Gustav Wieds vej 10, DK-8000 Aarhus C, Denmark.
authors.forename: Louise
authors.initials: L
authors.lastname: Bundgaard
authors.affiliation: Department of Large Animal Sciences, Veterinary School of Medicine, Faculty of Health and Medical Sciences, University of Copenhagen, Hoejbakkegaard Allé 5, DK-2630 Taastrup, Denmark.
authors.forename: Mette Aamand
authors.initials: MA
authors.lastname: Sørensen
authors.affiliation: Department of Large Animal Sciences, Veterinary School of Medicine, Faculty of Health and Medical Sciences, University of Copenhagen, Hoejbakkegaard Allé 5, DK-2630 Taastrup, Denmark.
authors.forename: Pia Haubro
authors.initials: PH
authors.lastname: Andersen
authors.affiliation: Department of Molecular Biology and Genetics, Aarhus University, Gustav Wieds vej 10, DK-8000 Aarhus C, Denmark.
authors.forename: Emøke
authors.initials: E
authors.lastname: Bendixen
mesh_terms: D000818:Animals; D002853:Chromatography, Liquid / Q000662:veterinary*; D006734:Horse Diseases / Q000276:immunology* / Q000378:metabolism; D006736:Horses; D007249:Inflammation / Q000276:immunology / Q000378:metabolism / Q000662:veterinary*; D020543:Proteome; D012463:Saliva / Q000737:chemistry; D053719:Tandem Mass Spectrometry / Q000662:veterinary*
chemical_list: D020543:Proteome
keywords: Equine; Inflammation; Mass spectrometry; Proteomics; Saliva