title: Yolk-shell nanostructured Fe3O4@NiSiO3 for selective affinity and magnetic separation of His-tagged proteins.
abstract: Recent developments of nanotechnology encourage novel materials for facile separations and purifications of recombinant proteins, which are of great importance in disease diagnoses and treatments. We find that Fe3O4@NiSiO3 with yolk-shell nanostructure can be used to specifically purify histidine-tagged (His-tagged) proteins from mixtures of lysed cells with a recyclable process. Each individual nanoparticle composes by a mesoporous nickel silicate shell and a magnetic Fe3O4 core in the hollow inner, which is featured by its great loading efficiency and rapid response toward magnetic fields. The abundant Ni(2+) cations on the shell provide docking sites for selective coordination of histidine and the reversible release is induced by excess imidazole solution. Because of the Fe3O4 cores, the separation, concentration, and recycling of the nanocomposites become feasible under the controls of magnets. These characteristics would be highly beneficial in nanoparticle-based biomedical applications for targeted-drug delivery and biosensors.
authors.affiliation: Center for Biomaterials and Biopathways, Department of Chemistry, Zhejiang University , Hangzhou, Zhejiang 310027, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Wang
authors.forename: Guangchuan
authors.initials: G
authors.lastname: Wang
authors.forename: Yun
authors.initials: Y
authors.lastname: Xiao
authors.forename: Yuling
authors.initials: Y
authors.lastname: Yang
authors.forename: Ruikang
authors.initials: R
authors.lastname: Tang
mesh_terms: D002470:Cell Survival / Q000187:drug effects; D017743:Equipment Reuse; D056945:Hep G2 Cells; D006639:Histidine; D006801:Humans; D058185:Magnetite Nanoparticles / Q000737:chemistry* / Q000633:toxicity; D009532:Nickel / Q000737:chemistry* / Q000633:toxicity; D009842:Oligopeptides; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000302:isolation & purification*; D013447:Sulfites / Q000737:chemistry* / Q000633:toxicity
chemical_list: C471213:His-His-His-His-His-His; D058185:Magnetite Nanoparticles; D009842:Oligopeptides; D011993:Recombinant Fusion Proteins; D013447:Sulfites; D006639:Histidine; C091887:nickel sulfite; D009532:Nickel
keywords: magnetic nanocomposites; protein separation; recyclability; yolk−shell