title: High-throughput gene expression profiling of opioid-induced alterations in discrete brain areas.
abstract: Whole-genome screening methods are unique approach to search for novel genes and molecular pathways involved in drug action. High-throughput profiling allows the gene expression levels of tens of thousands of transcripts to be measured simultaneously. Here, we describe transcriptional profiling in a specific area of the brain using DNA microarrays and next-generation sequencing.
authors.affiliation: Department of Molecular Neuropharmacology, Institute of Pharmacology, Polish Academy of Sciences, Smętna 12, 31343, Krakow, Poland.
authors.forename: Michal
authors.initials: M
authors.lastname: Korostynski
authors.forename: Marcin
authors.initials: M
authors.lastname: Piechota
authors.forename: Slawomir
authors.initials: S
authors.lastname: Golda
authors.forename: Ryszard
authors.initials: R
authors.lastname: Przewlocki
mesh_terms: D000701:Analgesics, Opioid / Q000008:administration & dosage / Q000378:metabolism*; D001921:Brain / Q000187:drug effects*; D001931:Brain Mapping; D020869:Gene Expression Profiling / Q000379:methods*; D005786:Gene Expression Regulation / Q000187:drug effects*; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods
chemical_list: D000701:Analgesics, Opioid