title: Peptidomics study of anthocyanin-rich juice of elderberry.
abstract: Biologically active peptides play a role in plant signaling and defense. Elderberry juice is known to contain a variety of anthocyanin compounds, a sub-set of polyphenols, which are responsible for the deep purple color of the juice. In this paper, we describe a method utilizing solid phase extraction (SPE) to remove anthocyanins from peptides. Liquid chromatography coupled with tandem mass spectrometry was used to separate and identify the peptides. The results showed that the use of SPE was an effective method to separate peptides from anthocyanins and other background compounds including high polyphenol content in the juice samples. More than 1000 peptides present in elderberry juice were successfully identified.
authors.affiliation: Department of Chemistry, Hong Kong Baptist University, Kowloon Tong, Hong Kong SAR, PR of China; Department of Chemistry, University of Missouri, Columbia, MO, USA; Center for Botanical Interaction Studies, University of Missouri, Columbia, MO, USA.
authors.forename: Hanzhi
authors.initials: H
authors.lastname: Wu
authors.affiliation: Department of Chemistry, University of Missouri, Columbia, MO, USA; Center for Botanical Interaction Studies, University of Missouri, Columbia, MO, USA.
authors.forename: Mitch C
authors.initials: MC
authors.lastname: Johnson
authors.affiliation: Center for Botanical Interaction Studies, University of Missouri, Columbia, MO, USA; Division of Animal Sciences, University of Missouri, Columbia, MO, USA.
authors.forename: Chi-Hua
authors.initials: CH
authors.lastname: Lu
authors.affiliation: Center for Botanical Interaction Studies, University of Missouri, Columbia, MO, USA; Division of Animal Sciences, University of Missouri, Columbia, MO, USA.
authors.forename: Kevin L
authors.initials: KL
authors.lastname: Fritsche
authors.affiliation: Center for Botanical Interaction Studies, University of Missouri, Columbia, MO, USA; Southwest Research Center, University of Missouri, Columbia, MO, USA.
authors.forename: Andrew L
authors.initials: AL
authors.lastname: Thomas
authors.affiliation: Department of Chemistry, Hong Kong Baptist University, Kowloon Tong, Hong Kong SAR, PR of China.
authors.forename: Yongquan
authors.initials: Y
authors.lastname: Lai
authors.affiliation: Department of Chemistry, Hong Kong Baptist University, Kowloon Tong, Hong Kong SAR, PR of China. Electronic address: greenliefm@missouri.edu.
authors.forename: Zongwei
authors.initials: Z
authors.lastname: Cai
authors.affiliation: Department of Chemistry, University of Missouri, Columbia, MO, USA; Center for Botanical Interaction Studies, University of Missouri, Columbia, MO, USA. Electronic address: zwcai@hkbu.edu.hk.
authors.forename: C Michael
authors.initials: CM
authors.lastname: Greenlief
mesh_terms: D000872:Anthocyanins / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid; D010446:Peptide Fragments / Q000032:analysis* / Q000302:isolation & purification*; D059808:Polyphenols / Q000302:isolation & purification; D027923:Sambucus / Q000737:chemistry*; D052616:Solid Phase Extraction / Q000379:methods*; D053719:Tandem Mass Spectrometry
chemical_list: D000872:Anthocyanins; D010446:Peptide Fragments; D059808:Polyphenols
keywords: Anthocyanin sambucus; Elderberry; Orbitrap-MS/MS; Peptidomics; Solid phase extraction