title: Evaluation of murine macrophage cytokine production after in vivo morphine treatment.
abstract: The discovery of opioid receptors expression on immune cells has originated a large research activity on the possible modulation by opioid drugs of immune system responses. In the present chapter we describe an easy methodology useful to obtain information about the potential immunomodulatory activity of opioid drugs. An in vivo treatment schedule is used, and macrophages are studied for their ability to release different cytokines.
authors.affiliation: Department of Pharmacological and Biomolecular Science, University of Milano, Via Vanvitelli 32, Milan, 20129, Italy.
authors.forename: Silvia
authors.initials: S
authors.lastname: Franchi
authors.forename: Mara
authors.initials: M
authors.lastname: Castelli
authors.forename: Sarah
authors.initials: S
authors.lastname: Moretti
authors.forename: Alberto
authors.initials: A
authors.lastname: Panerai
authors.forename: Paola
authors.initials: P
authors.lastname: Sacerdote
mesh_terms: D000818:Animals; D016207:Cytokines / Q000302:isolation & purification / Q000378:metabolism*; D007107:Immune System / Q000378:metabolism; D056747:Immunomodulation; D008264:Macrophages / Q000276:immunology / Q000378:metabolism*; D051379:Mice; D009020:Morphine / Q000008:administration & dosage; D011957:Receptors, Opioid / Q000276:immunology / Q000378:metabolism*
chemical_list: D016207:Cytokines; D011957:Receptors, Opioid; D009020:Morphine