title: Protective effect of ethyl pyruvate on ischemia-reperfusion injury in rat ovary: biochemical and histopathological evaluation.
abstract: BACKGROUND
  To investigate the protective effect of ethyl pyruvate (EP), an anti-oxidant agent, against ischemia-reperfusion injury in a rat model of ovarian torsion, considering biochemical and histopathological aspects.
  
  
  METHODS
  Forty Wistar Albino rats were divided into five groups: Group I, sham operation; Group II, ischemia alone, Group III, ischemia-reperfusion; Group IV, ischemia-reperfusion+50 mg/kg EP; and Group V, ischemia-reperfusion+100 mg/kg EP. Through laparotomy, 360° torsion was performed and maintained for 3 h, and detorsion was maintained for a further 3 h. Intraperitoneal EP was given 30 min before the surgical procedure. Ovarian tissues and blood samples were obtained after surgery. Malondialdehyde (MDA) and asymmetric dimethyl arginine (ADMA) levels were measured in both plasma and tissue samples. Tissue sections were evaluated histopatologically. Analysis of variance was used for statistical analyses.
  
  
  RESULTS
  In both serum and tissue samples, ADMA and MDA levels were found to be significantly lower in the EP groups compared with the ischemia alone and ischemia-reperfusion groups (p=0.0001). However, no significant difference was found between groups treated with 50 mg/kg or 100 mg/kg EP. On histopathological evaluation, the total tissue injury score was found to be lower in rats treated with EP. No significant difference was detected between groups treated with 50 mg/kg or 100 mg/kg EP.
  
  
  CONCLUSIONS
  Ethyl pyruvate may exert positive effects in ischemia-reperfusion injury in cases of ovarian torsion. However, no difference was detected between 50 mg/kg and 100 mg/kg EP.
authors.affiliation: Department of Obstetrics and Gynaecology, School of Medicine, Bozok University, Yozgat, Turkey. Electronic address: emelkiyak@hotmail.com.
authors.forename: Emel Kiyak
authors.initials: EK
authors.lastname: Caglayan
authors.affiliation: Department of Surgery, School of Medicine, Bozok University, Yozgat, Turkey.
authors.forename: Kasim
authors.initials: K
authors.lastname: Caglayan
authors.affiliation: Department of Biochemistry, School of Medicine, Bozok University, Yozgat, Turkey.
authors.forename: Ayse Yesim
authors.initials: AY
authors.lastname: Göcmen
authors.affiliation: Department of Surgery, Siirt Kurtalan State Hospital, Siirt, Turkey.
authors.forename: Hamza
authors.initials: H
authors.lastname: Cinar
authors.affiliation: Department of Obstetrics and Gynaecology, School of Medicine, Bozok University, Yozgat, Turkey.
authors.forename: Levent
authors.initials: L
authors.lastname: Seckin
authors.affiliation: Department of Pathology, School of Medicine, Bozok University, Yozgat, Turkey.
authors.forename: Selda
authors.initials: S
authors.lastname: Seckin
authors.affiliation: Department of Surgery, School of Medicine, Ondokuz Mayis University, Samsun, Turkey.
authors.forename: Bülent
authors.initials: B
authors.lastname: Güngör
authors.affiliation: Department of Biochemistry, School of Medicine, Bozok University, Yozgat, Turkey.
authors.forename: Muhammet Fevzi
authors.initials: MF
authors.lastname: Polat
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage / Q000627:therapeutic use*; D001120:Arginine / Q000031:analogs & derivatives / Q000378:metabolism; D004195:Disease Models, Animal; D005260:Female; D008315:Malondialdehyde / Q000378:metabolism; D010053:Ovary / Q000098:blood supply* / Q000378:metabolism; D011773:Pyruvates / Q000008:administration & dosage / Q000627:therapeutic use*; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000209:etiology / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D014102:Torsion Abnormality / Q000150:complications*
chemical_list: D000975:Antioxidants; D011773:Pyruvates; C487735:dimethylarginine; C046522:ethyl pyruvate; D008315:Malondialdehyde; D001120:Arginine
keywords: Ethyl pyruvate; Ovary; Torsion