title: An improved surface passivation method for single-molecule studies.
abstract: We report a surface passivation method based on dichlorodimethylsilane (DDS)-Tween-20 for in vitro single-molecule studies, which, under the conditions tested here, more efficiently prevented nonspecific binding of biomolecules than the standard poly(ethylene glycol) surface. The DDS-Tween-20 surface was simple and inexpensive to prepare and did not perturb the behavior and activities of tethered biomolecules. It can also be used for single-molecule imaging in the presence of high concentrations of labeled species in solution.
authors.affiliation: Center for Biophysics and Computational Biology, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA.
authors.forename: Boyang
authors.identifier: 0000000288699762
authors.initials: B
authors.lastname: Hua
authors.affiliation: 1] Department of Physics, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA. [2] Center for the Physics of Living Cells, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA. [3] Howard Hughes Medical Institute, Urbana, Illinois, USA.
authors.forename: Kyu Young
authors.initials: KY
authors.lastname: Han
authors.affiliation: 1] Department of Physics, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA. [2] Center for the Physics of Living Cells, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA.
authors.forename: Ruobo
authors.initials: R
authors.lastname: Zhou
authors.affiliation: 1] School of Life Sciences, Ulsan National Institute of Science and Technology, Ulsan, Republic of Korea. [2] Center for Soft and Living Matter, Institute for Basic Science, Ulsan, Republic of Korea.
authors.forename: Hajin
authors.initials: H
authors.lastname: Kim
authors.affiliation: 1] Howard Hughes Medical Institute, Urbana, Illinois, USA. [2] Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA.
authors.forename: Xinghua
authors.initials: X
authors.lastname: Shi
authors.affiliation: T.C. Jenkins Department of Biophysics, Johns Hopkins University, Baltimore, Maryland, USA.
authors.forename: Sanjaya C
authors.initials: SC
authors.lastname: Abeysirigunawardena
authors.affiliation: Center for Biophysics and Computational Biology, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA.
authors.forename: Ankur
authors.initials: A
authors.lastname: Jain
authors.affiliation: Center for Biophysics and Computational Biology, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA.
authors.forename: Digvijay
authors.initials: D
authors.lastname: Singh
authors.affiliation: Center for Biophysics and Computational Biology, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA.
authors.forename: Vasudha
authors.initials: V
authors.lastname: Aggarwal
authors.affiliation: 1] T.C. Jenkins Department of Biophysics, Johns Hopkins University, Baltimore, Maryland, USA. [2] Program in Cell, Molecular, Developmental Biology, and Biophysics (CMDB), Johns Hopkins University, Baltimore, Maryland, USA.
authors.forename: Sarah A
authors.identifier: 0000000301701987
authors.initials: SA
authors.lastname: Woodson
authors.affiliation: 1] Center for Biophysics and Computational Biology, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA. [2] Department of Physics, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA. [3] Center for the Physics of Living Cells, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA. [4] Howard Hughes Medical Institute, Urbana, Illinois, USA. [5] Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA.
authors.forename: Taekjip
authors.identifier: 0000000321956258
authors.initials: T
authors.lastname: Ha
mesh_terms: D004259:DNA-Directed DNA Polymerase / Q000378:metabolism*; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D006801:Humans; D011092:Polyethylene Glycols / Q000737:chemistry* / Q000378:metabolism; D011136:Polysorbates / Q000737:chemistry* / Q000378:metabolism; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism*; D012821:Silanes / Q000737:chemistry* / Q000378:metabolism; D013499:Surface Properties; D000099270:Y-Family DNA Polymerases
chemical_list: D004259:DNA-Directed DNA Polymerase; D011092:Polyethylene Glycols; D011136:Polysorbates; D018809:Proliferating Cell Nuclear Antigen; D012821:Silanes; C040863:dichlorodimethylsilane; C404302:POLK protein, human; D000099270:Y-Family DNA Polymerases