title: Indazole-type alkaloids from Nigella sativa seeds exhibit antihyperglycemic effects via AMPK activation in vitro.
abstract: Six rare naturally occurring indazole-type alkaloids including two new compounds, 17-O-(β-d-glucopyranosyl)-4-O-methylnigellidine (1) and nigelanoid (2), and four known compounds (3-6) were isolated from a defatted extract of Nigella sativa (black cumin) seeds. 17-O-(β-d-Glucopyranosyl)-4-O-methylnigellidine (1) increased glucose consumption by liver hepatocytes (HepG2 cells) through activation of AMP-activated protein kinase (AMPK). Also, this is the first report of compounds 4 and 6 from a natural source.
authors.affiliation: The Key Laboratory of Plant Resources and Chemistry of Arid Zone, and State Key Laboratory Basis of Xinjiang Indigenous Medicinal Plants Resource Utilization, Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences , Urumqi 830011, People's Republic of China.
authors.forename: Tao
authors.initials: T
authors.lastname: Yuan
authors.forename: Pragati
authors.initials: P
authors.lastname: Nahar
authors.forename: Meenakshi
authors.initials: M
authors.lastname: Sharma
authors.forename: Ke
authors.initials: K
authors.lastname: Liu
authors.forename: Angela
authors.initials: A
authors.lastname: Slitt
authors.forename: H A
authors.initials: HA
authors.lastname: Aisa
authors.forename: Navindra P
authors.initials: NP
authors.lastname: Seeram
mesh_terms: D055372:AMP-Activated Protein Kinases / Q000378:metabolism*; D000470:Alkaloids / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D056945:Hep G2 Cells; D022781:Hepatocytes / Q000378:metabolism; D006801:Humans; D007004:Hypoglycemic Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D007191:Indazoles / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D015394:Molecular Structure; D031881:Nigella sativa / Q000737:chemistry*; D019906:Nuclear Magnetic Resonance, Biomolecular; D012639:Seeds / Q000737:chemistry
chemical_list: D000470:Alkaloids; D007004:Hypoglycemic Agents; D007191:Indazoles; D055372:AMP-Activated Protein Kinases