title: New types of wheat chromosomal structural variations in derivatives of wheat-rye hybrids.
abstract: BACKGROUND
  Chromosomal rearrangements induced by wheat-rye hybridization is a very well investigated research topic. However, the structural alterations of wheat chromosomes in wheat-rye hybrids are seldom reported.
  
  
  RESULTS
  Octoploid triticale lines were derived from common wheat Triticum. aestivum L. 'Mianyang11'×rye Secale cereale L. 'Kustro'. Some progeny were obtained by the controlled backcrossing of triticale with 'Mianyang11' and common wheat T. aestivum L. 'Chuannong27' followed by self-fertilization. Fluorescence in situ hybridization (FISH) and genomic in situ hybridization (GISH) using Oligo-pSc119.2-1, Oligo-pTa535-1 and rye genomic DNA as probes were used to analyze the mitotic chromosomes of these progeny. Alterations of wheat chromosomes including 5A, 6A, 1B, 2B, 6B, 7B, 1D, 3D and 7D were observed. 5AL arm carrying intercalary Oligo-pSc119.2-1, Oligo-pTa535-1 or both Oligo-pSc119.2-1 and Oligo-pTa535-1 signals, 6AS, 1BS and 1DL arms containing terminal Oligo-pSc119.2-1 signal, 6BS and 3DS arms without terminal Oligo-pSc119.2-1 signal, 7BS without subtelomeric Oligo-pSc119.2-1 signal and 7DL with intercalary Oligo-pSc119.2-1 signal have been observed. However, these changed wheat chromosomes have not been detected in 'Mianyang11' and Chuannong 27. The altered 5A, 6A, 7B and 7D chromosomes in this study have not been reported and represent several new karyotype structures of common wheat chromosomes.
  
  
  CONCLUSIONS
  These rearranged wheat chromosomes in the present study afford some new genetic variations for wheat breeding program and are valuable materials for studying the biological function of tandem repetitive DNA sequences.
authors.affiliation: Agronomy College, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, China.
authors.forename: Zongxiang
authors.initials: Z
authors.lastname: Tang
authors.affiliation: Agronomy College, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, China.
authors.forename: Meng
authors.initials: M
authors.lastname: Li
authors.affiliation: Agronomy College, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Chen
authors.affiliation: Agronomy College, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, China.
authors.forename: Yangyang
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Agronomy College, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, China.
authors.forename: Zhenglong
authors.initials: Z
authors.lastname: Ren
authors.affiliation: Agronomy College, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, China.
authors.forename: Shulan
authors.initials: S
authors.lastname: Fu
mesh_terms: D002678:Chimera; D002869:Chromosome Aberrations; D032461:Chromosomes, Plant; D017404:In Situ Hybridization, Fluorescence; D012434:Secale / Q000235:genetics*; D014908:Triticum / Q000235:genetics*