title: Biogenic gold nano-triangles: cargos for anticancer drug delivery.
abstract: We present synthesis of biogenic gold nano triangles (GNTs) using Azadirachta indica leaf extract at inherent pH (5.89) and its application in efficient drug delivery of doxorubicin (DOX) (anticancer drug). The main idea was to take advantage of large surface area of GNTs which has 3 dimensions and use the plant peptides coated on these triangles as natural linkers for the attachment of DOX. Sucrose density gradient centrifugation (SDGC) and dialysis methods were used for separation of the GNT from mixture of GNPs. Flocculation parameter (FP) was used to check stability of GNT which was found to be exceptionally high (0-0.75) due to the biological capping agents. DOX attachment to GNT was verified using Fourier transformed infra-red (FTIR) spectroscopy. The complex thus formed was found to be less toxic to normal cells (MDCK cells) and significantly toxic for the cancerous cells (HeLa cells). Drug loading efficiency was found to be 99.81% and DOX release followed first order release kinetics. Percentage drug release was found to be more than 4.5% in both acidic (5.8) as well as physiological pH (7.2) which is suitable for tumor targeting.
authors.affiliation: N.S.N. Research Center for Nanotechnology and Bio-Nanotechnology, Ambernath, MS, India.
authors.forename: Roopa
authors.initials: R
authors.lastname: Dharmatti
authors.affiliation: N.S.N. Research Center for Nanotechnology and Bio-Nanotechnology, Ambernath, MS, India.
authors.forename: Chinmay
authors.initials: C
authors.lastname: Phadke
authors.affiliation: N.S.N. Research Center for Nanotechnology and Bio-Nanotechnology, Ambernath, MS, India.
authors.forename: Ashmi
authors.initials: A
authors.lastname: Mewada
authors.affiliation: N.S.N. Research Center for Nanotechnology and Bio-Nanotechnology, Ambernath, MS, India.
authors.forename: Mukeshchand
authors.initials: M
authors.lastname: Thakur
authors.affiliation: N.S.N. Research Center for Nanotechnology and Bio-Nanotechnology, Ambernath, MS, India.
authors.forename: Sunil
authors.initials: S
authors.lastname: Pandey
authors.affiliation: N.S.N. Research Center for Nanotechnology and Bio-Nanotechnology, Ambernath, MS, India. Electronic address: sharonmadhuri@gmail.com.
authors.forename: Madhuri
authors.initials: M
authors.lastname: Sharon
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology*; D031601:Azadirachta / Q000737:chemistry; D001672:Biocompatible Materials / Q000737:chemistry; D004285:Dogs; D004317:Doxorubicin / Q000737:chemistry / Q000494:pharmacology; D004337:Drug Carriers / Q000737:chemistry*; D006046:Gold / Q000737:chemistry / Q000494:pharmacology*; D006367:HeLa Cells; D006801:Humans; D006863:Hydrogen-Ion Concentration; D061985:Madin Darby Canine Kidney Cells; D053768:Metal Nanoparticles / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology
chemical_list: D000970:Antineoplastic Agents; D001672:Biocompatible Materials; D004337:Drug Carriers; D010936:Plant Extracts; D006046:Gold; D004317:Doxorubicin
keywords: Biogenic-synthesis; Cytotoxicity; Drug release kinetics; Gold nano triangles; HeLa cells; MDCK cells