title: Comparative proteomics analysis of Bacillus amyloliquefaciens SQR9 revealed the key proteins involved in in situ root colonization.
abstract: Bacillus Amyloliquefaciens SQR9 is a well-investigated plant growth-promoting rhizobacteria with strong root colonization capability. To identify the key proteins involved in in situ root colonization and biofilm formation, the proteomic profiles of planktonic and root colonized SQR9 cells were compared. A total of 755 proteins were identified, of which 78 and 95 proteins were significantly increased and deceased, respectively, when SQR9 was colonized on the root. The proteins that were closely affiliated with the root colonization belonged to the functional categories of biocontrol, detoxification, biofilm formation, cell motility and chemotaxis, transport, and degradation of plant polysaccharides. A two-component system protein ResE was increased 100-fold when compared to the planktonic status; impairment of the resE gene postponed the formation of cell biofilm and decreased the root colonization capability, which may be regulated through the spo0A-sinI-yqxM pathway. The SQR9 proteomic data provide valuable clues for screening key proteins in the plant-rhizobacteria interaction.
authors.affiliation: National Engineering Research Center for Organic-Based Fertilizers and Jiangsu Collaborative Innovation Center for Solid Organic Waste Resource Utilization, Nanjing Agricultural University , Nanjing 210095, P.R. China.
authors.forename: Meihua
authors.initials: M
authors.lastname: Qiu
authors.forename: Zhihui
authors.initials: Z
authors.lastname: Xu
authors.forename: Xingxing
authors.initials: X
authors.lastname: Li
authors.forename: Qing
authors.initials: Q
authors.lastname: Li
authors.forename: Nan
authors.initials: N
authors.lastname: Zhang
authors.forename: Qirong
authors.initials: Q
authors.lastname: Shen
authors.forename: Ruifu
authors.initials: R
authors.lastname: Zhang
mesh_terms: D001407:Bacillus / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D018441:Biofilms; D018553:Cucumis sativus / Q000382:microbiology*; D015870:Gene Expression; D020543:Proteome / Q000235:genetics / Q000378:metabolism*; D040901:Proteomics; D027343:Rhizome / Q000382:microbiology*
chemical_list: D001426:Bacterial Proteins; D020543:Proteome
keywords: biofilm formation; proteomics; resE; root colonization