title: Allergenicity of two Anisakis simplex allergens evaluated in vivo using an experimental mouse model.
abstract: Anisakis (Anisakidae) is one of the most important causes of helminth-induced allergic reactions and elicits clinical responses that include urticaria, rhinitis, bronco-constriction, cough, and/or gastrointestinal symptoms. More than 13 reactive allergens have been identified in the serum of Anisakis allergy patients, but the allergenicity of only a few of these have been evaluated in vivo using a mouse model. To evaluate the allergenicity of two important allergens, Ani s 1 and Ani s 9, we induced experimental allergic airway inflammation in a mouse model by repeated intranasal administration of the allergens. Both recombinant proteins (rAni s 1 and rAni s 9) elicited increased airway hyperresponsivity, airway infiltration by inflammatory cells (especially eosinophils), bronchial epithelial cell hyperplasia, all of which are characteristic of allergic airway inflammation. These allergens significantly increased the levels of Th2-related cytokines (IL-4, IL-5, IL-13, and IL-25) and Th17 related cytokines (IL-6 and IL-17) in both splenocytes and airway (except IL-17 in airway by rAni s 9). OVA-specific IgE and total IgE were increased in rAni s 1 and rAni s 9 treated mice as compared with controls treated with OVA alone. In addition, these two allergens induced gene expression of thymic stromal lymphopoietin (TSLP) and IL-25 (initiators of the Th2 response), as well as CXCL1 (initiator of the Th17 response) in mouse lung epithelial cells. In conclusion, repeated intranasal treatments with rAni s 1 and rAni s 9 induced airway inflammation in mice by elevating of Th2 and Th17 responses in the lung.
authors.affiliation: Department of Parasitology, School of Medicine, Pusan National University, Yangsan, Gyeongsangnam-do 626-870, Republic of Korea.
authors.forename: Min Kyoung
authors.initials: MK
authors.lastname: Cho
authors.affiliation: Department of Parasitology, School of Medicine, Pusan National University, Yangsan, Gyeongsangnam-do 626-870, Republic of Korea; Immunoregulatory Therapeutics Group in Brain Busan 21 Project, Busan, Republic of Korea.
authors.forename: Mi Kyung
authors.initials: MK
authors.lastname: Park
authors.affiliation: Department of Parasitology, School of Medicine, Pusan National University, Yangsan, Gyeongsangnam-do 626-870, Republic of Korea; Immunoregulatory Therapeutics Group in Brain Busan 21 Project, Busan, Republic of Korea.
authors.forename: Shin Ae
authors.initials: SA
authors.lastname: Kang
authors.affiliation: Department of Immunology, Hospital Carlos III, Madrid, Bilbao, Spain.
authors.forename: Maria Luisa
authors.initials: ML
authors.lastname: Caballero
authors.affiliation: Department of Immunology, Hospital Carlos III, Madrid, Bilbao, Spain.
authors.forename: Teresa
authors.initials: T
authors.lastname: Perez-Pinar
authors.affiliation: Department of Immunology, Hospital Carlos III, Madrid, Bilbao, Spain.
authors.forename: Rosa
authors.initials: R
authors.lastname: Rodriguez-Perez
authors.affiliation: Department of Parasitology, Kosin University College of Medicine, Busan 602-703, Republic of Korea.
authors.forename: Mee Sun
authors.initials: MS
authors.lastname: Ock
authors.affiliation: Department of Parasitology, Kosin University College of Medicine, Busan 602-703, Republic of Korea.
authors.forename: Hee Jae
authors.initials: HJ
authors.lastname: Cha
authors.affiliation: Department of Parasitology, School of Medicine, Kyungpook National University, Daegu 700-422, Republic of Korea.
authors.forename: Yeon Chul
authors.initials: YC
authors.lastname: Hong
authors.affiliation: Department of Parasitology, School of Medicine, Pusan National University, Yangsan, Gyeongsangnam-do 626-870, Republic of Korea; Immunoregulatory Therapeutics Group in Brain Busan 21 Project, Busan, Republic of Korea. Electronic address: hsyu@pusan.ac.kr.
authors.forename: Hak Sun
authors.initials: HS
authors.lastname: Yu
mesh_terms: D000485:Allergens / Q000235:genetics / Q000276:immunology*; D000818:Animals; D017163:Anisakis / Q000235:genetics / Q000276:immunology*; D016535:Bronchial Hyperreactivity / Q000209:etiology; D001992:Bronchoalveolar Lavage Fluid / Q000276:immunology; D016207:Cytokines / Q000032:analysis / Q000378:metabolism; D004195:Disease Models, Animal; D005260:Female; D015801:Helminth Proteins / Q000235:genetics / Q000276:immunology; D007249:Inflammation / Q000209:etiology / Q000276:immunology; D008168:Lung / Q000276:immunology / Q000473:pathology; D051379:Mice; D008810:Mice, Inbred C57BL; D011994:Recombinant Proteins / Q000276:immunology; D012137:Respiratory System / Q000276:immunology* / Q000473:pathology; D013047:Specific Pathogen-Free Organisms; D013154:Spleen / Q000166:cytology / Q000276:immunology
chemical_list: D000485:Allergens; D016207:Cytokines; D015801:Helminth Proteins; D011994:Recombinant Proteins
keywords: Airway allergen; Ani s 1; Ani s 9; Anisakis simplex; Intra nasal