title: Simultaneous enrichment and separation of flavonoids from Herba Epimedii by macroporous resins coupled with preparative chromatographic method.
abstract: An efficient, feasible enrichment and separation method of epimedins A, B, C and icariin from Herba Epimedii was developed by the combination of microwave-assisted extraction, macroporous resins and preparative HPLC. WDX-5 macroporous resin shows better recoveries at 96.2%, 97.0%, 98.2% and 97.1% for epimedins A, B, C and icariin than other macroporous resins used in the experiments. As a result, epimedins A (5.1 mg), B (15.3 mg), C (7.6 mg) and icariin (14.3 mg) were obtained from 6.0 g crude Herba Epimedii with the recoveries at 70.8%, 68.9%, 66.7% and 95.3%, respectively. The method developed in this study may provide scientific references for the enrichment and separation of flavonoids from Herba Epimedii.
authors.affiliation: a Department of Natural Medicinal Chemistry , China Pharmaceutical University , 24 Tong Jia Xiang, Nanjing 210009 , P.R. China.
authors.forename: Zhengzheng
authors.initials: Z
authors.lastname: Zhou
authors.forename: Jianguang
authors.initials: J
authors.lastname: Luo
authors.forename: Junsong
authors.initials: J
authors.lastname: Wang
authors.forename: Liang
authors.initials: L
authors.lastname: Li
authors.forename: Lingyi
authors.initials: L
authors.lastname: Kong
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D034181:Epimedium / Q000737:chemistry*; D005419:Flavonoids / Q000302:isolation & purification*; D015394:Molecular Structure
chemical_list: D005419:Flavonoids; C529106:epimedin A; C106053:epimedin B; C106054:epimedin C; C056599:icariin
keywords: Epimedium; epimedin A; epimedin B; epimedin C; icariin; separation