title: Rapid identif ication and comparative analysis of chemical constituents in herbal medicine Fufang decoction by ultra-high-pressure liquid chromatography coupled with a hybrid linear ion trap-high-resolution mass spectrometry.
abstract: This study was conducted to reveal the relation between herbal medicine Fufang decoction and a single drug in terms of material base. Da-Cheng-Qi decoction (DCQD) was used as a model. Ultrahigh-pressure liquid chromatography coupled with a hybrid linear ion trap-high-resolution mass spectrometry (UHPLC-LTQ-Orbitrap) was applied to detect and identify the main chemical compounds. This technique was also employed to determine the different chemical components. Under optimized liquid chromatography and mass spectrometry conditions, 64 components, including iridoids, flavonoids, anthraquinones and coumarins, were separated and tentatively characterized in Da-Cheng-Qi decoction. After decoction, the contents of 18 compounds were markedly changed, and two components were no longer detected in Fufang decoction compared with single-medicine decoction. The established method provided a good example for the rapid identification of complicated polar constituents in herbal medicine prescriptions.
authors.affiliation: Research Center of TCM Processing Technology, Zhejiang Chinese Medical University, Hangzhou, China.
authors.forename: Gang
authors.initials: G
authors.lastname: Cao
authors.forename: Xiaocheng
authors.initials: X
authors.lastname: Chen
authors.forename: Xin
authors.initials: X
authors.lastname: Wu
authors.forename: Qinglin
authors.initials: Q
authors.lastname: Li
authors.forename: Hongyan
authors.initials: H
authors.lastname: Zhang
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D029001:Herbal Medicine; D013058:Mass Spectrometry / Q000379:methods*; D015394:Molecular Structure; D010946:Plants, Medicinal / Q000737:chemistry*
chemical_list: D004365:Drugs, Chinese Herbal
keywords: Fufang decoction; chemical component; co-decoction; mixed decoction; ultrahigh-pressure liquid chromatography coupled with a hybrid linear ion trap-high resolution mass spectrometry