title: Occurrence of dsRNA Mycovirus (LeV-FMRI0339) in the Edible Mushroom Lentinula edodes and Meiotic Stability of LeV-FMRI0339 among Monokaryotic Progeny.
abstract: dsRNA was found in malformed cultures of Lentinula edodes strain FMRI0339, one of the three most popular sawdust cultivated commercial strains of shiitake, and was also found in healthy-looking fruiting bodies and actively growing mycelia. Cloning of the partial genome of the dsRNA revealed the presence of the RdRp sequence of a novel L. edodes mycovirus (LeV), and sequence comparison of the cloned amplicon showed identical sequences sequence to known RNA-dependent RNA polymerase genes of LeV found in strain HKA. The meiotic stability of dsRNA was examined by measuring the ratio of the presence of dsRNA among sexual monokaryotic progeny. More than 40% of the monokaryotic progeny still contained the dsRNA, indicating the persistence of dsRNA during sexual reproduction. Comparing the mycelia growth of monokaryotic progeny suggested that there appeared to be a tendency toward a lower frequency of virus incidence in actively growing progeny.
authors.affiliation: Department of Bio-Environmental Chemistry, Wonkwang University, Iksan, Chonbuk 570-749, Korea.
authors.forename: Jung-Mi
authors.initials: JM
authors.lastname: Kim
authors.affiliation: Department of Molecular Biology, Department of Bioactive Material Sciences, Center for Fungal Pathogenesis, Chonbuk National University, Jeonju, Chonbuk 561-756, Korea.
authors.forename: Suk-Hyun
authors.initials: SH
authors.lastname: Yun
authors.affiliation: Department of Molecular Biology, Department of Bioactive Material Sciences, Center for Fungal Pathogenesis, Chonbuk National University, Jeonju, Chonbuk 561-756, Korea.
authors.forename: Seung-Moon
authors.initials: SM
authors.lastname: Park
authors.affiliation: Forest Mushroom Research Institute, Yeoju, Gyeonggi 469-803, Korea.
authors.forename: Han-Gyu
authors.initials: HG
authors.lastname: Ko
authors.affiliation: Department of Molecular Biology, Department of Bioactive Material Sciences, Center for Fungal Pathogenesis, Chonbuk National University, Jeonju, Chonbuk 561-756, Korea.
authors.forename: Dae-Hyuk
authors.initials: DH
authors.lastname: Kim
keywords: Lentinula edodes; dsRNA; meiotic stability; mycovirus