title: Evaluation of urinary prostaglandin E2 metabolite as a biomarker in infants with fever due to viral infection.
abstract: We have investigated the clinical feasibility of the major urinary metabolite of prostaglandin (PG) E2, tetranor-PGEM, as a biomarker of inflammation in infants with fever. We tested two different and clinically relevant sampling methods, using self-adhesive urinary bags or gauze pads, with respect to stability of tetranor-PGEM and ease of sampling from infants. Liquid chromatography tandem mass spectrometry (LC-MS/MS) analysis was used to quantify tetranor-PGEM in urine, and different normalization parameters, i.e., urinary creatinine and body surface area, were investigated. To study inflammation, infants (1 month-1 year) that were hospitalized with fever of unknown origin at admittance (n=14) were compared to age-matched healthy controls (n=14). Levels of urinary tetranor-PGEM in infants with viral induced fever were increased compared to controls (102.4±56.2 vs. 37.0±21.6pmol/ml/m(2) body surface area, p<0.001). We conclude that urinary tetranor-PGEM is a potential non-invasive biomarker of inflammation in infants.
authors.affiliation: Rheumatology Unit, Department of Medicine, Karolinska University Hospital, Sweden.
authors.forename: Helena
authors.initials: H
authors.lastname: Idborg
authors.affiliation: Rheumatology Unit, Department of Medicine, Karolinska University Hospital, Sweden.
authors.forename: Sven-Christian
authors.initials: SC
authors.lastname: Pawelzik
authors.affiliation: Neonatal Research Unit, Department of Women's and Children׳s Health, Astrid Lindgren Children׳s Hospital both at Karolinska Institutet, S-171 76, Stockholm, Sweden.
authors.forename: Monica
authors.initials: M
authors.lastname: Perez-Manso
authors.affiliation: Neonatal Research Unit, Department of Women's and Children׳s Health, Astrid Lindgren Children׳s Hospital both at Karolinska Institutet, S-171 76, Stockholm, Sweden.
authors.forename: Lars
authors.initials: L
authors.lastname: Björk
authors.affiliation: Neonatal Research Unit, Department of Women's and Children׳s Health, Astrid Lindgren Children׳s Hospital both at Karolinska Institutet, S-171 76, Stockholm, Sweden.
authors.forename: Johan
authors.initials: J
authors.lastname: Hamrin
authors.affiliation: Neonatal Research Unit, Department of Women's and Children׳s Health, Astrid Lindgren Children׳s Hospital both at Karolinska Institutet, S-171 76, Stockholm, Sweden. Electronic address: eric.herlenius@ki.se.
authors.forename: Eric
authors.initials: E
authors.lastname: Herlenius
authors.affiliation: Rheumatology Unit, Department of Medicine, Karolinska University Hospital, Sweden.
authors.forename: Per-Johan
authors.initials: PJ
authors.lastname: Jakobsson
mesh_terms: D015415:Biomarkers / Q000652:urine*; D002853:Chromatography, Liquid; D015232:Dinoprostone / Q000378:metabolism; D005260:Female; D005334:Fever / Q000652:urine / Q000821:virology*; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D011453:Prostaglandins / Q000652:urine*; D053719:Tandem Mass Spectrometry; D014777:Virus Diseases / Q000652:urine*
chemical_list: D015415:Biomarkers; D011453:Prostaglandins; C532049:tetranor prostaglandin M; D015232:Dinoprostone
keywords: Biomarker; Fever; Infants; Inflammation; Prostaglandin E(2) (PGE(2)); Urine