title: Rice, Japonica (Oryza sativa L.).
abstract: The importance of rice, as a food crop, is reflected in the extensive global research being conducted in an effort to improve and better understand this particular agronomic plant. In regard to biotechnology, this has led to the development of numerous genetic transformation protocols. Over the years, many of these methods have become increasingly straightforward, rapid, and efficient, thereby making rice valuable as a model crop for scientific research and functional genomics. The focus of this chapter is on one such protocol that uses Agrobacterium-mediated transformation of Oryza sativa L. ssp. Japonica cv. Nipponbare with an emphasis on tissue desiccation. The explants consist of callus derived from mature seeds which are cocultivated on filter paper postinfection. Hygromycin selection is used for the recovery of subsequent genetically engineered events.
authors.affiliation: Center for Plant Transformation, Plant Sciences Institute, and Department of Agronomy, Iowa State University, G405 AGRON, 2104 Agronomy Hall, Ames, IA, 50011-1010, USA, mmain@iastate.edu.
authors.forename: Marcy
authors.initials: M
authors.lastname: Main
authors.forename: Bronwyn
authors.initials: B
authors.lastname: Frame
authors.forename: Kan
authors.initials: K
authors.lastname: Wang
mesh_terms: D016960:Agrobacterium tumefaciens / Q000235:genetics*; D002934:Cinnamates / Q000494:pharmacology; D005821:Genetic Techniques; D006921:Hygromycin B / Q000031:analogs & derivatives / Q000494:pharmacology; D012275:Oryza / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development; D030821:Plants, Genetically Modified; D012639:Seeds / Q000235:genetics / Q000254:growth & development; D013242:Sterilization / Q000379:methods; D014169:Transformation, Bacterial
chemical_list: D002934:Cinnamates; D006921:Hygromycin B; C026273:hygromycin A