title: Pilot study of p62 DNA vaccine in dogs with mammary tumors.
abstract: Our previous data demonstrated profound anti-tumor and anti-metastatic effects of p62 (sqstm1) DNA vaccine in rodents with various types of transplantable tumors. Testing anti-cancer medicine in dogs as an intermediary step of translational research program provides two major benefits. First, clinical data collected in target animals is required for FDA/USDA approval as a veterinary anti-cancer drug or vaccine. It is noteworthy that the veterinary community is in need of novel medicine for the prevention and treatment of canine and feline cancers. The second more important benefit of testing anti-cancer vaccines in dogs is that spontaneous tumors in dogs may provide invaluable information for human trials. Here, we evaluated the effect(s) of p62 DNA vaccine on mammary tumors of dogs. We found that p62 DNA vaccine administered i.m. decreased or stabilized growth of locally advanced lesions in absence of its overall toxic effects. The observed antitumor activity was associated with lymphocyte infiltration and tumor encapsulation via fibrotic reaction. This data justifies both human clinical trials and veterinary application of p62 DNA vaccine.
authors.affiliation: CureLab Oncology Inc, Needham MA, USA.
authors.forename: Vladimir
authors.initials: V
authors.lastname: Gabai
authors.affiliation: School of Biosciences and Veterinary Medicine University of Camerino, Camerino (MC) Italy.
authors.forename: Franco M
authors.initials: FM
authors.lastname: Venanzi
authors.affiliation: Center of Modern Veterinary Medicine, Kiev, Ukraine.
authors.forename: Elena
authors.initials: E
authors.lastname: Bagashova
authors.affiliation: Center of Modern Veterinary Medicine, Kiev, Ukraine.
authors.forename: Oksana
authors.initials: O
authors.lastname: Rud
authors.affiliation: School of Biosciences and Veterinary Medicine University of Camerino, Camerino (MC) Italy.
authors.forename: Francesca
authors.initials: F
authors.lastname: Mariotti
authors.affiliation: School of Biosciences and Veterinary Medicine University of Camerino, Camerino (MC) Italy.
authors.forename: Cecilia
authors.initials: C
authors.lastname: Vullo
authors.affiliation: School of Biosciences and Veterinary Medicine University of Camerino, Camerino (MC) Italy.
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Catone
authors.affiliation: Department Biochemistry, Boston University School of Medicine, Boston MA, USA.
authors.forename: Michael Y
authors.initials: MY
authors.lastname: Sherman
authors.affiliation: School of Biosciences and Veterinary Medicine University of Camerino, Camerino (MC) Italy.
authors.forename: Antonio
authors.initials: A
authors.lastname: Concetti
authors.affiliation: CureLab Oncology Inc, Needham MA, USA.
authors.forename: Andrey
authors.initials: A
authors.lastname: Chursov
authors.forename: Anastasia
authors.initials: A
authors.lastname: Latanova
authors.affiliation: CureLab Oncology Inc, Needham MA, USA. CL Oncology Moscow, Russia.
authors.forename: Vita
authors.initials: V
authors.lastname: Shcherbinina
authors.affiliation: CureLab Oncology Inc, Needham MA, USA.
authors.forename: Victor
authors.initials: V
authors.lastname: Shifrin
authors.affiliation: CureLab Oncology Inc, Needham MA, USA. CL Oncology Moscow, Russia.
authors.forename: Alexander
authors.initials: A
authors.lastname: Shneider
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000276:immunology*; D000818:Animals; D019496:Cancer Vaccines / Q000008:administration & dosage* / Q000276:immunology / Q000633:toxicity*; D004283:Dog Diseases / Q000628:therapy*; D004285:Dogs; D005260:Female; D006801:Humans; D008297:Male; D015674:Mammary Neoplasms, Animal / Q000628:therapy*; D010865:Pilot Projects; D000071456:Sequestosome-1 Protein; D018675:Toxicity Tests; D019444:Vaccines, DNA / Q000008:administration & dosage* / Q000276:immunology / Q000633:toxicity*
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D019496:Cancer Vaccines; C099718:SQSTM1 protein, human; D000071456:Sequestosome-1 Protein; D019444:Vaccines, DNA