title: Oridonin suppress cell migration via regulation of nonmuscle myosin IIA.
abstract: Oridonin, which is isolated from Chinese herb Rabdosia rubescens (Hemsl.) Hara, has been implicated in regulation of tumor cell migration and invasion. In this study, treatment with oridonin enhanced the phosphorylation of myosin regulatory light chain (T18/S19) that regulates the ATPase activity of myosin IIA. Meanwhile, stress fibers were significantly more prominent after oridonin incubation, which impaired cell migration in transwell migration assays. All of these effects may be caused by the decreased interaction between myosin IIA and myosin phosphatase complex, but not kinases. Our data provide clear evidence of this novel pharmacological function for oridonin in treating cancer cell migration.
authors.affiliation: School of Pharmaceutical Sciences, Zhengzhou University, 100 Kexue Avenue, Zhengzhou, 450001, People's Republic of China.
authors.forename: Yin-Chao
authors.initials: YC
authors.lastname: Li
authors.affiliation: School of Pharmaceutical Sciences, Zhengzhou University, 100 Kexue Avenue, Zhengzhou, 450001, People's Republic of China.
authors.forename: Mo-Ran
authors.initials: MR
authors.lastname: Sun
authors.affiliation: School of Pharmaceutical Sciences, Zhengzhou University, 100 Kexue Avenue, Zhengzhou, 450001, People's Republic of China.
authors.forename: Yi-Hong
authors.initials: YH
authors.lastname: Zhao
authors.affiliation: School of Pharmaceutical Sciences, Zhengzhou University, 100 Kexue Avenue, Zhengzhou, 450001, People's Republic of China.
authors.forename: Xian-Zu
authors.initials: XZ
authors.lastname: Fu
authors.affiliation: School of Pharmaceutical Sciences, Zhengzhou University, 100 Kexue Avenue, Zhengzhou, 450001, People's Republic of China. xhwzpu@126.com.
authors.forename: Hai-Wei
authors.initials: HW
authors.lastname: Xu
authors.affiliation: School of Pharmaceutical Sciences, Zhengzhou University, 100 Kexue Avenue, Zhengzhou, 450001, People's Republic of China. jifeng926@126.com.
authors.forename: Ji-Feng
authors.initials: JF
authors.lastname: Liu
keywords: Migration; Myosin IIA; Oridonin; Phosphatase complex; Regulatory light chain