title: Phytoremediation of phenanthrene by transgenic plants transformed with a naphthalene dioxygenase system from Pseudomonas.
abstract: Genes from microbes for degrading polycyclic aromatic hydrocarbons (PAHs) are seldom used to improve the ability of plants to remediate the pollution because the initiation of the microbial degradation of PAHs is catalyzed by a multienzyme system. In this study, for the first time, we have successfully transferred the complex naphthalene dioxygenase system of Pseudomonas into Arabidopsis and rice, the model dicot and monocot plant. As in bacteria, all four genes of the naphthalene dioxygenase system can be simultaneously expressed and assembled to an active enzyme in transgenic plants. The naphthalene dioxygenase system can develop the capacity of plants to tolerate a high concentration of phenanthrene and metabolize phenanthrene in vivo. As a result, transgenic plants showed improved uptake of phenanthrene from the environment over wild-type plants. In addition, phenanthrene concentrations in shoots and roots of transgenic plants were generally lower than that of wild type plants. Transgenic plants with a naphthalene dioxygenase system bring the promise of an efficient and environmental-friendly technology for cleaning up PAHs contaminated soil and water.
authors.affiliation: Agro-Biotechnology Research Institute, Shanghai Academy of Agricultural Sciences , 2901 Beidi Rd, Shanghai, 201106, People's Republic of China.
authors.forename: Ri-He
authors.initials: RH
authors.lastname: Peng
authors.forename: Xiao-Yan
authors.initials: XY
authors.lastname: Fu
authors.forename: Wei
authors.initials: W
authors.lastname: Zhao
authors.forename: Yong-Sheng
authors.initials: YS
authors.lastname: Tian
authors.forename: Bo
authors.initials: B
authors.lastname: Zhu
authors.forename: Hong-Juan
authors.initials: HJ
authors.lastname: Han
authors.forename: Jing
authors.initials: J
authors.lastname: Xu
authors.forename: Quan-Hong
authors.initials: QH
authors.lastname: Yao
mesh_terms: D000222:Adaptation, Physiological / Q000187:drug effects; D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics*; D001673:Biodegradation, Environmental / Q000187:drug effects; D049308:Dioxygenases / Q000378:metabolism*; D004787:Environmental Pollution / Q000032:analysis; D009097:Multienzyme Complexes / Q000378:metabolism*; D012275:Oryza / Q000187:drug effects / Q000502:physiology; D010616:Phenanthrenes / Q000378:metabolism* / Q000633:toxicity; D018517:Plant Roots / Q000187:drug effects / Q000378:metabolism; D018520:Plant Shoots / Q000187:drug effects / Q000378:metabolism; D030821:Plants, Genetically Modified; D011549:Pseudomonas / Q000201:enzymology*; D012987:Soil; D012989:Soil Pollutants / Q000633:toxicity; D014170:Transformation, Genetic; D014874:Water Pollutants, Chemical / Q000633:toxicity
chemical_list: D009097:Multienzyme Complexes; D010616:Phenanthrenes; D012987:Soil; D012989:Soil Pollutants; D014874:Water Pollutants, Chemical; C031181:phenanthrene; D049308:Dioxygenases; C038703:naphthalene dioxygenase