title: Genome Wide Mapping of Foxo1 Binding-sites in Murine T Lymphocytes.
abstract: The Forkhead box O (Foxo) family of transcription factors has a critical role in controlling the development, differentiation, and function of T cells. However, the direct target genes of Foxo transcription factors in T cells have not been well characterized. In this study, we focused on mapping the genome wide Foxo1-binding sites in naïve CD4+T cells, CD8+T cells, and Foxp3+regulatory T (Treg) cells. By using chromatin immunoprecipitation coupled with deep sequencing (ChIP-Seq), we identified Foxo1 binding sites that were shared among or specific to the three T cell populations. Here we describe the experiments, quality controls, as well as the deep sequencing data. Part of the data analysis has been published by Ouyang Wet al.in Nature 2012[1] and Kim MVet al.in Immunity 2013[2], and the associated data set were uploaded to NCBI Gene Expression Omnibus.
authors.affiliation: New York Genome Center, New York, NY 10013.
authors.forename: Will
authors.initials: W
authors.lastname: Liao
authors.affiliation: Immunology Program, Memorial Sloan-Kettering Cancer Center, New York, NY 10065.
authors.forename: Weiming
authors.initials: W
authors.lastname: Ouyang
authors.affiliation: Department of Molecular and Cell Biology, Center for Systems Biology, The University of Texas at Dallas, Richardson, TX 75080 ; Bioinformatics Division, Center for Synthetic and Systems Biology, TNLIST, Tsinghua University, Beijing, 100084, China.
authors.forename: Michael Q
authors.initials: MQ
authors.lastname: Zhang
authors.affiliation: Immunology Program, Memorial Sloan-Kettering Cancer Center, New York, NY 10065.
authors.forename: Ming O
authors.initials: MO
authors.lastname: Li