title: Enhanced production of xylanase from locally isolated fungal strain using agro-industrial residues under solid-state fermentation.
abstract: This study is related to the isolation of fungal strain for xylanase production using agro-industrial residues. Forty fungal strains with xylanolytic potential were isolated by using xylan agar plates and quantitatively screened in solid-state fermentation. Of all the tested isolates, the strain showing highest ability to produce xylanase was assigned the code Aspergillus niger LCBT-14. For the enhanced production of the enzyme, five different fermentation media were evaluated. Out of all media, M4 containing wheat bran gave maximum enzyme production. Effect of different variables including incubation time, temperature, pH, carbon and nitrogen sources has been investigated. The optimum enzyme production was obtained after 72 h at 30°C and pH 4. Glucose as a carbon source while ammonium sulphate and yeast extract as nitrogen sources gave maximum xylanase production (946 U/mL/min). This study was successful in producing xylanase by A. niger LCBT-14 economically by utilising cheap indigenous substrate.
authors.affiliation: a Department of Biotechnology , Lahore College for Women University , Lahore 54600 , Pakistan.
authors.forename: Roheena
authors.initials: R
authors.lastname: Abdullah
authors.forename: Kinza
authors.initials: K
authors.lastname: Nisar
authors.forename: Aafia
authors.initials: A
authors.lastname: Aslam
authors.forename: Mehwish
authors.initials: M
authors.lastname: Iqtedar
authors.forename: Shagufta
authors.initials: S
authors.lastname: Naz
mesh_terms: D001234:Aspergillus niger / Q000201:enzymology*; D003470:Culture Media; D005285:Fermentation; D007218:Industrial Microbiology; D014995:Xylosidases / Q000096:biosynthesis*
chemical_list: D003470:Culture Media; D014995:Xylosidases
keywords: Aspergillus niger; solid-state fermentation; xylanase