title: Halenaquinone inhibits RANKL-induced osteoclastogenesis.
abstract: Halenaquinone was isolated from the marine sponge Petrosia alfiani as an inhibitor of osteoclastogenic differentiation of murine RAW264 cells. It inhibited the RANKL (receptor activator of nuclear factor-κB ligand)-induced upregulation of TRAP (tartrate-resistant acid phosphatase) activity as well as the formation of multinuclear osteoclasts. In addition, halenaquinone substantially suppressed RANKL-induced IκB degradation and Akt phosphorylation. Thus, these results suggest that halenaquinone inhibits RANKL-induced osteoclastogenesis at least by suppressing the NF-κB and Akt signaling pathways.
authors.affiliation: Graduate School of Pharmaceutical Sciences, Kumamoto University, 5-1 Oe-honmachi, Kumamoto 862-0973, Japan. Electronic address: sachiko@kumamoto-u.ac.jp.
authors.forename: Sachiko
authors.initials: S
authors.lastname: Tsukamoto
authors.affiliation: Faculty of Pharmaceutical Sciences, Josai University, 1-1 Keyakidai, Sakado, Saitama 350-0295, Japan.
authors.forename: Tomoharu
authors.initials: T
authors.lastname: Takeuchi
authors.affiliation: Graduate School of Pharmaceutical Sciences, Kumamoto University, 5-1 Oe-honmachi, Kumamoto 862-0973, Japan.
authors.forename: Tetsuro
authors.initials: T
authors.lastname: Kawabata
authors.affiliation: Graduate School of Pharmaceutical Sciences, Kumamoto University, 5-1 Oe-honmachi, Kumamoto 862-0973, Japan.
authors.forename: Hikaru
authors.initials: H
authors.lastname: Kato
authors.affiliation: Graduate School of Pharmaceutical Sciences, Kumamoto University, 5-1 Oe-honmachi, Kumamoto 862-0973, Japan.
authors.forename: Michiko
authors.initials: M
authors.lastname: Yamakuma
authors.affiliation: Graduate School of Pharmaceutical Sciences, Kumamoto University, 5-1 Oe-honmachi, Kumamoto 862-0973, Japan.
authors.forename: Kanae
authors.initials: K
authors.lastname: Matsuo
authors.affiliation: Graduate School of Pharmaceutical Sciences, Kumamoto University, 5-1 Oe-honmachi, Kumamoto 862-0973, Japan.
authors.forename: Ahmed H
authors.initials: AH
authors.lastname: El-Desoky
authors.affiliation: Faculty of Fisheries and Marine Science, Sam Ratulangi University, Kampus Bahu, Manado 95115, Indonesia.
authors.forename: Fitje
authors.initials: F
authors.lastname: Losung
authors.affiliation: Faculty of Fisheries and Marine Science, Sam Ratulangi University, Kampus Bahu, Manado 95115, Indonesia.
authors.forename: Remy E P
authors.initials: RE
authors.lastname: Mangindaan
authors.affiliation: Naturalis Biodiversity Center, PO Box 9517, 2300 RA Leiden, The Netherlands.
authors.forename: Nicole J
authors.initials: NJ
authors.lastname: de Voogd
authors.affiliation: Faculty of Pharmaceutical Sciences, Josai University, 1-1 Keyakidai, Sakado, Saitama 350-0295, Japan.
authors.forename: Yoichiro
authors.initials: Y
authors.lastname: Arata
authors.affiliation: School of Pharmacy, Aichi Gakuin University, Chikusa-ku, Nagoya 464-8650, Japan.
authors.forename: Hideyoshi
authors.initials: H
authors.lastname: Yokosawa
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D020672:I-kappa B Proteins / Q000378:metabolism; D051379:Mice; D010010:Osteoclasts / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D010012:Osteogenesis / Q000187:drug effects; D047130:Petrosia / Q000737:chemistry / Q000378:metabolism; D010766:Phosphorylation / Q000187:drug effects; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D011809:Quinones / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D053245:RANK Ligand / Q000633:toxicity; D015398:Signal Transduction / Q000187:drug effects
chemical_list: D020672:I-kappa B Proteins; D011809:Quinones; D053245:RANK Ligand; C430084:halenaquinone; D051057:Proto-Oncogene Proteins c-akt
keywords: Halenaquinone; Marine sponge; Osteoclastogenesis; Petrosia alfiani