title: Effect of low-intensity pulsed ultrasound on biocompatibility and cellular uptake of chitosan-tripolyphosphate nanoparticles.
abstract: Using low molecular weight chitosan nanoparticles (CNPs) prepared by an ionic gelation method, the authors report the effect of low-intensity pulsed ultrasound (US) on cell viability and nanoparticle uptake in cultured murine preosteoblasts. Particle size and zeta potential are measured using dynamic light scattering, and cell viability is evaluated using the of [3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, inner salt; MTS] assay. Results show that 30 min delivery of CNPs at 0.5 mg/mL is able to prevent loss of cell viability due to either serum starvation or subsequent exposure to US (1 W/cm(2) or 2 W/cm(2), up to 1 min). Additionally, flow cytometry data suggest that there is a close association between cellular membrane integrity and the presence of CNPs when US at 2 W/cm(2) is administered.
authors.affiliation: Department of Materials Science and Engineering, Stony Brook University, Stony Brook, New York 11794.
authors.forename: Junyi
authors.initials: J
authors.lastname: Wu
authors.affiliation: Department of Materials Science and Engineering, Stony Brook University, Stony Brook, New York 11794.
authors.forename: Gaojun
authors.initials: G
authors.lastname: Liu
authors.affiliation: Department of Biomedical Engineering, Stony Brook University, Stony Brook, New York 11794.
authors.forename: Yi-Xian
authors.initials: YX
authors.lastname: Qin
authors.affiliation: Department of Materials Science and Engineering, Stony Brook University, 314 Old Engineering, Stony Brook, New York 11794.
authors.forename: Yizhi
authors.initials: Y
authors.lastname: Meng
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000378:metabolism; D002462:Cell Membrane / Q000502:physiology / Q000528:radiation effects; D002470:Cell Survival; D002478:Cells, Cultured; D048271:Chitosan / Q000378:metabolism*; D004705:Endocytosis; D051379:Mice; D053758:Nanoparticles / Q000378:metabolism*; D010006:Osteoblasts / Q000378:metabolism / Q000502:physiology* / Q000528:radiation effects*; D011122:Polyphosphates / Q000378:metabolism*; D013016:Sound; D013234:Stem Cells / Q000378:metabolism / Q000502:physiology* / Q000528:radiation effects*
chemical_list: D001672:Biocompatible Materials; D011122:Polyphosphates; D048271:Chitosan; C005692:triphosphoric acid