title: Fabrication and characterization of chitosan-collagen crosslinked membranes for corneal tissue engineering.
abstract: This article describes a chitosan-collagen composite membrane as corneal tissue-engineering biomaterials. The membrane was prepared by dissolving the chitosan into collagen with the weight ratio of 0, 15, 30, 45, 60, and 100%, followed by crosslinked with 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide. Mechanical properties, contact angles, and optical transmittance were determined and compared between chitosan membrane and crosslinking composite membrane. As a result, the optical transparency and mechanical strength of the chitosan-collagen membranes were significantly better than that of the sample of chitosan. In addition, in vitro cell culture studies revealed that the collagen has no negative effect on the cell morphology, viability, and proliferation and possess good biocompatibility. Overall, the dendrimer crosslinked chitosan-collagen composite membranes showed promising properties that suggest that these might be suitable biomaterials for corneal tissue-engineering applications.
authors.affiliation: a School of Materials Science and Engineering , South China University of Technology , Guangzhou 510641 , P.R. China.
authors.forename: Weichang
authors.initials: W
authors.lastname: Li
authors.forename: Yuyu
authors.initials: Y
authors.lastname: Long
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.forename: Kai
authors.initials: K
authors.lastname: Long
authors.forename: Sa
authors.initials: S
authors.lastname: Liu
authors.forename: Zhichong
authors.initials: Z
authors.lastname: Wang
authors.forename: Yingjun
authors.initials: Y
authors.lastname: Wang
authors.forename: Li
authors.initials: L
authors.lastname: Ren
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry* / Q000494:pharmacology*; D002448:Cell Adhesion / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D048271:Chitosan / Q000737:chemistry*; D003094:Collagen / Q000737:chemistry*; D003315:Cornea / Q000166:cytology*; D006801:Humans; D055595:Mechanical Phenomena; D008567:Membranes, Artificial; D055620:Optical Phenomena; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry
chemical_list: D001672:Biocompatible Materials; D008567:Membranes, Artificial; D003094:Collagen; D048271:Chitosan
keywords: 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC); chitosan; collagen; crosslinked membranes