title: Analysis of microRNA-203 function in CREB/MITF/RAB27a pathway: comparison between canine and human melanoma cells.
abstract: MicroRNA (miR)-203 is downregulated and acts as an anti-oncomir in melanoma cells. Here, using human and canine melanoma cells, we elucidated the effects of miR-203 on cyclic adenosine monophosphate response element binding protein (CREB)/microphthalmia-associated transcription factor (MITF)/RAB27a pathway, which is known to be important for the development and progression of human melanoma. In this study, we showed that miR-203 directly targeted CREB1 and regulated its downstream targets, MITF and RAB27a. miR-203 significantly suppressed the growth of human and canine melanoma cells and inhibited melanosome transport through the suppression of the signalling pathway. In conclusion, miR-203 was shown to be a common tumour-suppressive miRNA in human and canine melanoma and thus to play a crucial role in the biological mechanisms of melanoma development.
authors.affiliation: Laboratory of Molecular Diagnostic and Therapeutics, Joint faculty of Veterinary Medicine, Yamaguchi University, Yamaguchi, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Noguchi
authors.affiliation: The United Graduate School of Drug Discovery and Medical Information Sciences, Gifu University, Gifu, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Kumazaki
authors.affiliation: Laboratory of Veterinary Clinical Oncology, Faculty of Applied Biological Sciences, Gifu University, Gifu, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Mori
authors.affiliation: Laboratory of Veterinary Internal Medicine, Joint Faculty of Veterinary Medicine, Yamagushi University, Yamaguchi, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Baba
authors.affiliation: Laboratory of Veterinary Internal Medicine, Joint Faculty of Veterinary Medicine, Yamagushi University, Yamaguchi, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Okuda
authors.affiliation: Laboratory of Molecular Diagnostic and Therapeutics, Joint faculty of Veterinary Medicine, Yamaguchi University, Yamaguchi, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Mizuno
authors.affiliation: The United Graduate School of Drug Discovery and Medical Information Sciences, Gifu University, Gifu, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Akao
mesh_terms: D000818:Animals; D017362:Cyclic AMP Response Element-Binding Protein / Q000235:genetics / Q000378:metabolism*; D004283:Dog Diseases / Q000378:metabolism*; D004285:Dogs; D015972:Gene Expression Regulation, Neoplastic / Q000502:physiology*; D006801:Humans; D008545:Melanoma / Q000378:metabolism; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D051739:Microphthalmia-Associated Transcription Factor / Q000235:genetics / Q000378:metabolism*; D013045:Species Specificity; D020691:rab GTP-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D000075745:rab27 GTP-Binding Proteins
chemical_list: D017362:Cyclic AMP Response Element-Binding Protein; C546703:MIRN203 microRNA, human; D035683:MicroRNAs; D051739:Microphthalmia-Associated Transcription Factor; D000075745:rab27 GTP-Binding Proteins; C066694:RAB27A protein, human; D020691:rab GTP-Binding Proteins
keywords: CREB/MITF/RAB27a pathway; cell signalling; comparative oncology; melanoma; microRNA-203