title: Simultaneous determination of seven phenolic acids in three Salvia species by capillary zone electrophoresis with β-cyclodextrin as modifier.
abstract: A capillary zone electrophoresis method was developed for the simultaneous determination of seven phenolic acids, including protocatechuic aldehyde (1), salvianolic acid C (2), rosmarinic acid (3), salvianolic acid A (4), danshensu (5), salvianolic acid B (6), and protocatechuic acid (7), in Danshen and related medicinal plants. A running buffer composed of 20 mM sodium tetraborate adjusted to pH 9.0, and containing 12 mM β-cyclodextrin as modifier. Baseline separation was achieved within 17 min running at the voltage of 20 kV, temperature of 25°C and detection wavelength of 280 nm. The relative standard deviations of migration time ranged from 0.2 to 0.7% and the peak area ranged from 1.5 to 3.7% for the seven analytes, indicating the good repeatability of the proposed method. The method was extensively validated by evaluating the linearity (R(2) ≥ 0.9992), limits of detection (0.14-0.36 μg/mL), limits of quantification (0.47-1.19 μg/mL), and recovery (96.0-102.6%). Under the optimum conditions, samples of Danshen and related medicinal plants were analyzed using the developed method with high separation efficiency.
authors.affiliation: State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macau, China.
authors.forename: Jiliang
authors.initials: J
authors.lastname: Cao
authors.forename: Jinchao
authors.initials: J
authors.lastname: Wei
authors.forename: Ke
authors.initials: K
authors.lastname: Tian
authors.forename: Huanxing
authors.initials: H
authors.lastname: Su
authors.forename: Jianbo
authors.initials: J
authors.lastname: Wan
authors.forename: Peng
authors.initials: P
authors.lastname: Li
mesh_terms: D019075:Electrophoresis, Capillary; D062385:Hydroxybenzoates / Q000032:analysis*; D015394:Molecular Structure; D027544:Salvia / Q000737:chemistry*; D047392:beta-Cyclodextrins / Q000737:chemistry*
chemical_list: D062385:Hydroxybenzoates; D047392:beta-Cyclodextrins; C017616:phenolic acid; C031215:betadex
keywords: Capillary zone electrophoresis; Danshen; Phenolic acids; β-cyclodextrin