title: Characterization of pantropic canine coronavirus from Brazil.
abstract: Characterization of canine coronavirus (CCoV) strains currently in circulation is essential for understanding viral evolution. The aim of this study was to determine the presence of pantropic CCoV type IIa in tissue samples from five puppies that died in Southern Brazil as a result of severe gastroenteritis. Reverse-transcriptase PCR was used to generate amplicons for sequence analysis. Phylogenetic analysis of the CCoV-IIa strains indicated that they were similar to those found in other countries, suggesting a common ancestor of these Brazilian isolates. This is the first report of pantropic CCoV-II in puppies from Latin America and our findings highlight that CCoV should be included as a differential diagnosis when dogs present with clinical signs and lesions typically seen with canine parvovirus infection.
authors.affiliation: Laboratório de Virologia, Faculdade de Veterinária, Universidade Federal do Rio Grande do Sul, Av. Bento Gonçalves, 9090, CEP 91540-000, Porto Alegre, RS, Brazil.
authors.forename: Luciane D
authors.initials: LD
authors.lastname: Pinto
authors.affiliation: Departamento de Medicina Veterinária Preventiva e Saúde Animal, Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo, Av. Prof. Dr. Orlando Marques de Paiva, 87, CEP 05508-270, São Paulo, SP, Brazil.
authors.forename: Iracema N
authors.initials: IN
authors.lastname: Barros
authors.affiliation: Laboratório de Virologia, Faculdade de Veterinária, Universidade Federal do Rio Grande do Sul, Av. Bento Gonçalves, 9090, CEP 91540-000, Porto Alegre, RS, Brazil.
authors.forename: Renata F
authors.initials: RF
authors.lastname: Budaszewski
authors.affiliation: Laboratório de Virologia, Faculdade de Veterinária, Universidade Federal do Rio Grande do Sul, Av. Bento Gonçalves, 9090, CEP 91540-000, Porto Alegre, RS, Brazil.
authors.forename: Matheus N
authors.initials: MN
authors.lastname: Weber
authors.affiliation: Laboratório de Virologia, Faculdade de Veterinária, Universidade Federal do Rio Grande do Sul, Av. Bento Gonçalves, 9090, CEP 91540-000, Porto Alegre, RS, Brazil.
authors.forename: Helena
authors.initials: H
authors.lastname: Mata
authors.affiliation: Laboratório de Virologia, Faculdade de Veterinária, Universidade Federal do Rio Grande do Sul, Av. Bento Gonçalves, 9090, CEP 91540-000, Porto Alegre, RS, Brazil.
authors.forename: Jéssica R
authors.initials: JR
authors.lastname: Antunes
authors.affiliation: Setor de Patologia, Faculdade de Veterinária, Universidade Federal do Rio Grande do Sul, Av. Bento Gonçalves, 9090, CEP 91540-000, Porto Alegre, RS, Brazil.
authors.forename: Fabiana M
authors.initials: FM
authors.lastname: Boabaid
authors.affiliation: Setor de Patologia, Faculdade de Veterinária, Universidade Federal do Rio Grande do Sul, Av. Bento Gonçalves, 9090, CEP 91540-000, Porto Alegre, RS, Brazil.
authors.forename: Angélica T B
authors.initials: AT
authors.lastname: Wouters
authors.affiliation: Setor de Patologia, Faculdade de Veterinária, Universidade Federal do Rio Grande do Sul, Av. Bento Gonçalves, 9090, CEP 91540-000, Porto Alegre, RS, Brazil.
authors.forename: David
authors.initials: D
authors.lastname: Driemeier
authors.affiliation: Departamento de Medicina Veterinária Preventiva e Saúde Animal, Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo, Av. Prof. Dr. Orlando Marques de Paiva, 87, CEP 05508-270, São Paulo, SP, Brazil.
authors.forename: Paulo E
authors.initials: PE
authors.lastname: Brandão
authors.affiliation: Laboratório de Virologia, Faculdade de Veterinária, Universidade Federal do Rio Grande do Sul, Av. Bento Gonçalves, 9090, CEP 91540-000, Porto Alegre, RS, Brazil. Electronic address: claudio.canal@ufrgs.br.
authors.forename: Cláudio W
authors.initials: CW
authors.lastname: Canal
mesh_terms: D000818:Animals; D001938:Brazil; D018352:Coronavirus Infections / Q000662:veterinary* / Q000821:virology; D017939:Coronavirus, Canine / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D004283:Dog Diseases / Q000821:virology*; D004285:Dogs; D005243:Feces / Q000821:virology; D005838:Genotype; D008969:Molecular Sequence Data; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics; D017423:Sequence Analysis, RNA / Q000662:veterinary; D014763:Viral Matrix Proteins / Q000235:genetics*
chemical_list: D012367:RNA, Viral; D014763:Viral Matrix Proteins
keywords: Canine coronavirus; Canine parvovirus; Dog; Pantropic; Sequencing