title: Distinct characteristics and complex evolution of PEDV strains, North America, May 2013-February 2014.
abstract: Porcine epidemic diarrhea virus (PEDV), which emerged in the United States in 2013, has spread throughout North America. Limited availability of PEDV complete genomes worldwide has impeded our understanding of PEDV introduction into the United States. To determine the relationship between the North American strains and global emerging and historic PEDV strains, we sequenced and analyzed complete genomes of 74 strains from North America; the strains clustered into 2 distinct clades. Compared with the initially reported virulent US PEDV strains, 7 (9.7%) strains from 4 states contained insertions and deletions in the spike gene (S INDELs). These S INDEL strains share 99.8%-100% nt identity with each other and 96.2%-96.7% nt identity with the initial US strains. Furthermore, the S INDEL strains form a distinct cluster within North American clade II, sharing 98.6%-100% nt identity overall. In the United States, the S INDEL and original PEDV strains are co-circulating and could have been introduced simultaneously.
authors.forename: Anastasia N
authors.initials: AN
authors.lastname: Vlasova
authors.forename: Douglas
authors.initials: D
authors.lastname: Marthaler
authors.forename: Qiuhong
authors.initials: Q
authors.lastname: Wang
authors.forename: Marie R
authors.initials: MR
authors.lastname: Culhane
authors.forename: Kurt D
authors.initials: KD
authors.lastname: Rossow
authors.forename: Albert
authors.initials: A
authors.lastname: Rovira
authors.forename: James
authors.initials: J
authors.lastname: Collins
authors.forename: Linda J
authors.initials: LJ
authors.lastname: Saif
mesh_terms: D000818:Animals; D005075:Biological Evolution; D018352:Coronavirus Infections / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology; D014644:Genetic Variation; D016679:Genome, Viral; D009656:North America / Q000453:epidemiology; D016366:Open Reading Frames / Q000235:genetics; D053485:Porcine epidemic diarrhea virus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D016865:Reassortant Viruses; D013552:Swine; D013553:Swine Diseases / Q000453:epidemiology / Q000821:virology*; D013997:Time Factors; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D014764:Viral Proteins