title: Molecular recognition of HER-1 in whole-blood samples.
abstract: Multimode sensing was proposed for molecular screening and recognition of HER-1 in whole blood. The tools used for molecular recognition were platforms based on nanostructured materials such as the complex of Mn(III) with meso-tetra (4-carboxyphenyl) porphyrin, and maltodextrin (dextrose equivalence between 4 and 7), immobilized in diamond paste, graphite paste or C60 fullerene paste. The identification of HER-1 in whole-blood samples, at molecular level, is performed using stochastic mode and is followed by the quantification of it using stochastic and differential pulse voltammetry modes. HER-1 can be identified in the concentration range between 280 fg/ml and 4.86 ng/ml using stochastic mode, this making possible the early detection of cancers such as gastrointestinal, pancreatic and lung cancers. The recovery tests performed using whole-blood samples proved that the platforms can be used for identification and quantification of HER-1 with high sensitivity and reliability in such samples, these making them good molecular screening tools.
authors.affiliation: Laboratory of Electrochemistry and PATLAB, National Institute of Research for Electrochemistry and Condensed Matter, 202 Splaiul Independentei Strada, 060021, Bucharest-6, Romania; Faculty of Applied Chemistry and Material Science, Politehnica University of Bucharest, Bucharest, Romania.
authors.forename: Iuliana
authors.initials: I
authors.lastname: Moldoveanu
authors.forename: Camelia
authors.initials: C
authors.lastname: Stanciu Gavan
authors.forename: Raluca-Ioana
authors.initials: RI
authors.lastname: Stefan-van Staden
mesh_terms: D015374:Biosensing Techniques; D018130:Diamond / Q000737:chemistry / Q000378:metabolism; D066246:ErbB Receptors / Q000097:blood*; D037741:Fullerenes / Q000737:chemistry / Q000378:metabolism; D005770:Gastrointestinal Neoplasms / Q000097:blood / Q000175:diagnosis*; D006108:Graphite / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D008175:Lung Neoplasms / Q000097:blood / Q000175:diagnosis*; D049329:Nanostructures / Q000737:chemistry*; D010190:Pancreatic Neoplasms / Q000097:blood / Q000175:diagnosis*
chemical_list: D037741:Fullerenes; D018130:Diamond; D006108:Graphite; C512478:EGFR protein, human; D066246:ErbB Receptors
keywords: HER-1; early diagnosis; molecular recognition; molecular screening; multimode sensing