title: Protein kinase C activator, bryostatin-1, promotes exercise-dependent functional recovery in rats with cerebral infarction.
abstract: Recently, it has become widely known that neuronal reorganization in the perilesional cortex contributes to some improvement of hemiparesis after stroke. Here, the authors examined in vivo the effects of administration of bryostatin-1, an activator of protein kinase C, combined with voluntary exercise on functional recovery and on cortical phosphorylation of α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor subunit GluR1 after infarction.In behavioral evaluation, the mean latency until falling from a rotating rod in the group with exercise and administered agent at 8 days after infarction was significantly longer than that in the other groups. Although there were no significant changes in GluR1 phosphorylation between bryostatin-1 administration alone and the untreated groups, exercise induced an increase in phosphorylated-Ser845-GluR1. Moreover, combining exercise with administration led to increased phosphorylated-Ser831-GluR1.These results suggest that bryostatin-1 facilitated exercise-induced paralysis recovery, which is possibly mediated by synaptic plasticity related to an increase in synaptic transmission efficiency.
authors.affiliation: From the Division of Biochemistry, Fujita Memorial Nanakuri Institute (KM, SS, KS), and Department of Internal Medicine, Nanakuri Sanatorium (HW), Fujita Health University, Mie, Japan.
authors.forename: Kenmei
authors.initials: K
authors.lastname: Mizutani
authors.forename: Shigeru
authors.initials: S
authors.lastname: Sonoda
authors.forename: Hideaki
authors.initials: H
authors.lastname: Wakita
authors.forename: Kan
authors.initials: K
authors.lastname: Shimpo
mesh_terms: D000818:Animals; D015153:Blotting, Western; D054713:Bryostatins / Q000627:therapeutic use*; D002544:Cerebral Infarction / Q000628:therapy*; D003131:Combined Modality Therapy; D004789:Enzyme Activation; D008297:Male; D010766:Phosphorylation; D010805:Physical Conditioning, Animal; D011493:Protein Kinase C / Q000378:metabolism*; D017207:Rats, Sprague-Dawley; D018091:Receptors, AMPA / Q000378:metabolism
chemical_list: D054713:Bryostatins; D011493:Protein Kinase C; D018091:Receptors, AMPA; C046785:bryostatin 1