title: Ratiometric two-photon fluorescent probe for quantitative detection of β-galactosidase activity in senescent cells.
abstract: We reported a ratiometric two-photon fluorescent probe (SG1) for β-galactosidase (β-gal) and its application to quantitative detection of β-gal activity during cellular senescence in live cells and in aged tissues. This probe is characterized by a significant two-photon excited fluorescence, a marked blue-to-yellow emission color change in response to β-gal, easy loading, insensitivity to pH and reactive oxygen species (ROS), high photostability, and low cytotoxicity. In addition, we show that SG1 labeling is an effective tool for quantitative detection of senescence-associated β-gal activity at the subcellular level in situ. This finding demonstrates that SG1 will find useful applications in biomedical research, including studies of cell aging.
authors.affiliation: Department of Energy Systems Research, Ajou University , Suwon, Gyeonggi-do 443-749, Korea.
authors.forename: Hyo Won
authors.initials: HW
authors.lastname: Lee
authors.forename: Cheol Ho
authors.initials: CH
authors.lastname: Heo
authors.forename: Debabrata
authors.initials: D
authors.lastname: Sen
authors.forename: Hae-Ok
authors.initials: HO
authors.lastname: Byun
authors.forename: In Hae
authors.initials: IH
authors.lastname: Kwak
authors.forename: Gyesoon
authors.initials: G
authors.lastname: Yoon
authors.forename: Hwan Myung
authors.initials: HM
authors.lastname: Kim
mesh_terms: D016922:Cellular Senescence; D005456:Fluorescent Dyes / Q000737:chemistry*; D011874:Radiometry; D001616:beta-Galactosidase / Q000378:metabolism*
chemical_list: D005456:Fluorescent Dyes; D001616:beta-Galactosidase