title: Radical scavenging and antioxidant effects of Matricaria chamomilla polyphenolic-polysaccharide conjugates.
abstract: Matricaria chamomilla L. (MC), a member of the Asteraceae family, is one of the oldest medicinal plants, widely used worldwide for a variety of healing applications. Its recommendations, derived from both traditional and modern medicine, include numerous disorders such as inflammation, ulcers, wounds, gastrointestinal disorders, stomach ache, pharyngitis, rheumatic pain, as well as the other ailments. This work is focused on another aspect of the biological activity of chamomile polyphenolic-polysaccharide conjugates--their antioxidant properties in the protection of blood plasma components against in vitro oxidative stress. Measurements of DPPH and ABTS radical scavenging indicated considerable anti-free radical action of MC. Pre-incubation of blood plasma with MC considerably diminished the extent of ONOO(-)-induced oxidative modifications such as protein carbonyl groups, SH groups, 3-nitrotyrosine, as well as the formation of lipid hydroperoxides. The analysis of the FRAP assay result shows a considerable increase of ferric reducing ability of blood plasma in the presence of MC. The results obtained in this study indicate that polyphenolic-polysaccharide conjugates isolated from M. chamomilla substances possess antioxidant properties. The M. chamomilla macromolecular glycoconjugates may be useful in the creation of new natural-based medications or dietary supplements, helpful in the prevention and treatment of oxidative stress-mediated disorders.
authors.affiliation: Department of General Biochemistry, Faculty of Biology and Environmental Protection, University of Lodz, Pomorska 141/143, 90-236 Lodz, Poland; Regional Specialist Hospital in Wrocław, Research and Development Centre, H. M. Kamieńskiego 73a, 51-124 Wrocław, Poland. Electronic address: joannak@biol.uni.lodz.pl.
authors.forename: Joanna
authors.initials: J
authors.lastname: Kolodziejczyk-Czepas
authors.affiliation: Department of General Biochemistry, Faculty of Biology and Environmental Protection, University of Lodz, Pomorska 141/143, 90-236 Lodz, Poland; Regional Specialist Hospital in Wrocław, Research and Development Centre, H. M. Kamieńskiego 73a, 51-124 Wrocław, Poland.
authors.forename: Michal
authors.initials: M
authors.lastname: Bijak
authors.affiliation: Department of General Biochemistry, Faculty of Biology and Environmental Protection, University of Lodz, Pomorska 141/143, 90-236 Lodz, Poland; Regional Specialist Hospital in Wrocław, Research and Development Centre, H. M. Kamieńskiego 73a, 51-124 Wrocław, Poland; Department of Toxicology, Faculty of Pharmacy with Division of Medical Analytics, Wroclaw Medical University, Borowska 211, 50-556 Wrocław, Poland.
authors.forename: Joanna
authors.initials: J
authors.lastname: Saluk
authors.affiliation: Department of General Biochemistry, Faculty of Biology and Environmental Protection, University of Lodz, Pomorska 141/143, 90-236 Lodz, Poland; Regional Specialist Hospital in Wrocław, Research and Development Centre, H. M. Kamieńskiego 73a, 51-124 Wrocław, Poland.
authors.forename: Michal B
authors.initials: MB
authors.lastname: Ponczek
authors.affiliation: Department of General Biochemistry, Faculty of Biology and Environmental Protection, University of Lodz, Pomorska 141/143, 90-236 Lodz, Poland; Regional Specialist Hospital in Wrocław, Research and Development Centre, H. M. Kamieńskiego 73a, 51-124 Wrocław, Poland.
authors.forename: Halina M
authors.initials: HM
authors.lastname: Zbikowska
authors.affiliation: Department of General Biochemistry, Faculty of Biology and Environmental Protection, University of Lodz, Pomorska 141/143, 90-236 Lodz, Poland; Regional Specialist Hospital in Wrocław, Research and Development Centre, H. M. Kamieńskiego 73a, 51-124 Wrocław, Poland.
authors.forename: Pawel
authors.initials: P
authors.lastname: Nowak
authors.affiliation: Regional Specialist Hospital in Wrocław, Research and Development Centre, H. M. Kamieńskiego 73a, 51-124 Wrocław, Poland; Division of Organic and Pharmaceutical Technology, Faculty of Chemistry, Wrocław University of Technology, Wybrzeże Wyspiańskiego 29, 50-370 Wrocław, Poland.
authors.forename: Marta
authors.initials: M
authors.lastname: Tsirigotis-Maniecka
authors.affiliation: Regional Specialist Hospital in Wrocław, Research and Development Centre, H. M. Kamieńskiego 73a, 51-124 Wrocław, Poland; Division of Organic and Pharmaceutical Technology, Faculty of Chemistry, Wrocław University of Technology, Wybrzeże Wyspiańskiego 29, 50-370 Wrocław, Poland.
authors.forename: Izabela
authors.initials: I
authors.lastname: Pawlaczyk
mesh_terms: D000975:Antioxidants / Q000494:pharmacology*; D052160:Benzothiazoles / Q000737:chemistry; D001713:Biphenyl Compounds / Q000737:chemistry; D016166:Free Radical Scavengers / Q000494:pharmacology*; D006801:Humans; D015227:Lipid Peroxidation / Q000187:drug effects; D031168:Matricaria / Q000737:chemistry*; D010851:Picrates / Q000737:chemistry; D059808:Polyphenols / Q000494:pharmacology*; D011134:Polysaccharides / Q000494:pharmacology*; D020011:Protective Agents / Q000494:pharmacology; D013451:Sulfonic Acids / Q000737:chemistry
chemical_list: D000975:Antioxidants; D052160:Benzothiazoles; D001713:Biphenyl Compounds; D016166:Free Radical Scavengers; D010851:Picrates; D059808:Polyphenols; D011134:Polysaccharides; D020011:Protective Agents; D013451:Sulfonic Acids; C002502:2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid; C004931:1,1-diphenyl-2-picrylhydrazyl
keywords: Antioxidant activity; Matricaria chamomilla L.; Peroxynitrite