title: Novel characterization of bEnd.3 cells that express lymphatic vessel endothelial hyaluronan receptor-1.
abstract: Murine bEnd.3 endothelioma cell line has been widely used in vascular research and here we report the novel finding that bEnd.3 cells express lymphatic vessel endothelial hyaluronan receptor-1 (LYVE-1) and vascular endothelial growth factor receptor-3 (VEGFR-3). Moreover, these cells express progenitor cell markers of Sca-1 and CD133. Upon stimulation with tumor necrosis factor-alpha (TNF-alpha), the bEnd.3 cells demonstrate enhanced formation of capillary-type tubes, which express LYVE-1. As the bEnd.3 cell line is derived from murine endothelioma, we further examined human tissues of endothelioma and identified lymphatic vessels in the tumor samples which express both LYVE-1 and podoplanin. Moreover, a significantly higher number of lymphatic vessels were detected in the endothelioma samples compared with normal control. Taken together, this study not only redefines bEnd.3 cells for vascular research, but also indicates a broader category of human diseases that are associated with lymphatics, such as endothelioma.
authors.forename: D
authors.initials: D
authors.lastname: Yuen
authors.forename: R
authors.initials: R
authors.lastname: Leu
authors.forename: J
authors.initials: J
authors.lastname: Tse
authors.forename: S
authors.initials: S
authors.lastname: Wang
authors.forename: L L
authors.initials: LL
authors.lastname: Chen
authors.forename: L
authors.initials: L
authors.lastname: Chen
mesh_terms: D000071916:AC133 Antigen; D000818:Animals; D015703:Antigens, CD / Q000378:metabolism; D000950:Antigens, Ly / Q000378:metabolism; D045744:Cell Line, Tumor / Q000378:metabolism*; D006023:Glycoproteins / Q000378:metabolism*; D006801:Humans; D008202:Lymphangioma / Q000378:metabolism* / Q000473:pathology; D042601:Lymphatic Vessels / Q000378:metabolism* / Q000473:pathology; D008565:Membrane Proteins / Q000378:metabolism; D026901:Membrane Transport Proteins; D051379:Mice; D010455:Peptides / Q000378:metabolism; D013234:Stem Cells / Q000378:metabolism / Q000473:pathology; D040321:Vascular Endothelial Growth Factor Receptor-3 / Q000378:metabolism*; D033921:Vesicular Transport Proteins / Q000378:metabolism*
chemical_list: D000071916:AC133 Antigen; D015703:Antigens, CD; D000950:Antigens, Ly; D006023:Glycoproteins; D008565:Membrane Proteins; D026901:Membrane Transport Proteins; D010455:Peptides; D040321:Vascular Endothelial Growth Factor Receptor-3; D033921:Vesicular Transport Proteins; C119248:LYVE1 protein, human; C074650:Ly6a protein, mouse; C000606023:PROM1 protein, human; C000606024:Prom1 protein, mouse; C492273:LYVE1 protein, mouse