title: Gryllotalpicola reticulitermitis sp. nov., isolated from a termite gut.
abstract: Strain TS-56(T) was isolated from the gut of a wood-feeding termite, Reticulitermes chinensis Snyder. Phylogenetic analyses based on 16S rRNA gene sequences revealed that the strain represented a member of the genus Gryllotalpicola of the family Microbacteriaceae, with sequence similarities to other species of the genus ranging from 96.6 % to 97.8 %. The isolate was Gram-stain-positive, non-motile, with light yellow colonies and irregular short rod-shaped cells (0.4-0.6 µm in diameter, 0.6-1.0 µm in length). Growth of TS-56(T) occurred at 20-35 °C (optimum, 30 °C) and at pH 4.0-8.0 (optimum, pH 5.0). The peptidoglycan of TS-56(T) contained ornithine, glutamic acid, alanine, homoserine and glycine. The acyl type was acetyl. The most abundant cellular fatty acid of TS-56(T) was cyclohexyl-C17 : 0 (88.79 %). The respiratory menaquinone was MK-11. The polar lipid profile contained disphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, phosphatidylinositol and two unknown glycolipids. DNA of the type strain had a G+C content of 67.4 mol%. On the basis of the phylogenetic properties and phenotypic distinctiveness, TS-56(T) represents a novel species of the genus Gryllotalpicola, for which the name Gryllotalpicola reticulitermitis sp. nov. is proposed. The type strain is TS-56(T) ( = CGMCC 1.10363(T) = NBRC 109838(T)).
authors.affiliation: Hubei Key Laboratory of Genetic Regulation and Integrative Biology, Institute of Entomology, School of Life Sciences, Central China Normal University, Wuhan 430079, PR China.
authors.forename: Hao
authors.initials: H
authors.lastname: Fang
authors.affiliation: Hubei Key Laboratory of Genetic Regulation and Integrative Biology, Institute of Entomology, School of Life Sciences, Central China Normal University, Wuhan 430079, PR China.
authors.forename: Wanyu
authors.initials: W
authors.lastname: Lv
authors.affiliation: Graduate School of Chinese Academy of Science, Beijing 100049, PR China.|State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, PR China.
authors.forename: Zhou
authors.initials: Z
authors.lastname: Huang
authors.affiliation: State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, PR China.
authors.forename: Shuang-Jiang
authors.initials: SJ
authors.lastname: Liu
authors.affiliation: Hubei Key Laboratory of Genetic Regulation and Integrative Biology, Institute of Entomology, School of Life Sciences, Central China Normal University, Wuhan 430079, PR China.
authors.forename: Hong
authors.initials: H
authors.lastname: Yang
mesh_terms: D000192:Actinomycetales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D000818:Animals; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D041981:Gastrointestinal Tract / Q000382:microbiology; D006017:Glycolipids / Q000737:chemistry; D020049:Isoptera / Q000382:microbiology*; D008969:Molecular Sequence Data; D010457:Peptidoglycan / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D024482:Vitamin K 2 / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D006017:Glycolipids; D010457:Peptidoglycan; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2