title: Molecular pathotyping and phylogenesis of the first Newcastle disease virus strain isolated from backyard chickens in Qatar.
abstract: The first Newcastle disease virus (NDV) field strain (NDV-QC08), previously isolated and identified in Qatar, was further subjected to molecular characterization and phylogenic analysis at passage levels two. Initial amplification and detection of the complementary DNA (cDNA) fragment of the NDV RNA amnioallantoic fluid (AAF) extract demonstrated the positive virus identity using real-time reverse transcriptase chain reaction (RRT-PCR). Partial sequence of the totally amplified 216 bp of the functional cleavage region encoding the F gene retrieved (112)R-R-Q-K-R-F(117) amino acid motif indicating the velogenic nature of the strain, and confirming the previously investigated in-vivo pathotyping. Phylogenic analysis and genotypic clustering studies classified the strain under NDV subgenotype VIId.
authors.affiliation: Virology Unit, Veterinary Laboratory, Department of Animal Resources, P.O. Box 23211, Doha, Qatar, mismail_99@yahoo.com.
authors.forename: Mohamed
authors.initials: M
authors.lastname: Haroun
authors.forename: Khalid Abdelhakeem
authors.initials: KA
authors.lastname: Mohran
authors.forename: Mahmoud Mahmoud
authors.initials: MM
authors.lastname: Hassan
authors.forename: Nawal Mohamed
authors.initials: NM
authors.lastname: Abdulla
mesh_terms: D020816:Amino Acid Motifs / Q000235:genetics; D000818:Animals; D002645:Chickens / Q000821:virology*; D016000:Cluster Analysis; D018076:DNA, Complementary / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics; D005838:Genotype; D008460:Meat; D009521:Newcastle Disease / Q000821:virology; D009522:Newcastle disease virus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D010802:Phylogeny; D011780:Qatar; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014777:Virus Diseases / Q000821:virology*
chemical_list: D018076:DNA, Complementary; D004279:DNA, Viral