title: The Effects of Broccoli Sprout Extract Containing Sulforaphane on Lipid Peroxidation and Helicobacter pylori Infection in the Gastric Mucosa.
abstract: OBJECTIVE
  The aims of this study were to investigate whether a broccoli sprout extract containing sulforaphane (BSES) inhibited the Helicobacter pylori infection density and exerted an antioxidative effect on gastric mucosal damage.
  
  
  METHODS
  The enrolled subjects were randomized in a double-blinded manner into three groups. Finally, 33 H. pylori (+) BSES treatment subjects (group A), 28 H. pylori (+) placebo subjects (group B), and 28 H. pylori (-) BSES treatment subjects (group C) were studied. H. pylori infection density was indirectly quantified by a (13)C-urea breath test (UBT), and the ammonia concentration in gastric juice aspirates was measured through gastroscopic examination. Malondialdehyde (MDA), an oxidative damage biomarker, and reduced glutathione (GSH), an antioxidant biomarker, were measured in the gastric mucosa by an enzyme-linked immunosorbent assay.
  
  
  RESULTS
  BSES treatment did not significantly affect the UBT values or ammonia concentration in group A (p=0.634 and p=0.505, respectively). BSES treatment did significantly reduce mucosal MDA concentrations in group A (p<0.05) and group C (p<0.001), whereas the gastric mucosal GSH concentrations did not differ before and after treatment in any of the groups.
  
  
  CONCLUSIONS
  BSES did not inhibit the H. pylori infection density. However, BSES prevented lipid peroxidation in the gastric mucosa and may play a cytoprotective role in H. pylori-induced gastritis.
authors.affiliation: Departments of Internal Medicine, Kyung Hee University College of Medicine, Seoul, Korea.
authors.forename: Young Woon
authors.initials: YW
authors.lastname: Chang
authors.affiliation: Departments of Internal Medicine, Kyung Hee University College of Medicine, Seoul, Korea.
authors.forename: Jae Young
authors.initials: JY
authors.lastname: Jang
authors.affiliation: Departments of Surgery, Kyung Hee University College of Medicine, Seoul, Korea.
authors.forename: Yong Ho
authors.initials: YH
authors.lastname: Kim
authors.affiliation: Departments of Internal Medicine, Kyung Hee University College of Medicine, Seoul, Korea.
authors.forename: Jung-Wook
authors.initials: JW
authors.lastname: Kim
authors.affiliation: Departments of Internal Medicine, Kyung Hee University College of Medicine, Seoul, Korea.
authors.forename: Jae-Jun
authors.initials: JJ
authors.lastname: Shim
mesh_terms: D000328:Adult; D000641:Ammonia / Q000378:metabolism; D000975:Antioxidants / Q000494:pharmacology*; D015415:Biomarkers / Q000032:analysis; D001937:Brassica / Q000737:chemistry*; D001944:Breath Tests; D004311:Double-Blind Method; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005750:Gastric Juice / Q000201:enzymology; D005753:Gastric Mucosa / Q000187:drug effects* / Q000378:metabolism; D005978:Glutathione / Q000032:analysis; D016481:Helicobacter Infections / Q000188:drug therapy*; D016480:Helicobacter pylori; D006801:Humans; D017879:Isothiocyanates / Q000494:pharmacology*; D015227:Lipid Peroxidation / Q000187:drug effects*; D008297:Male; D008315:Malondialdehyde / Q000032:analysis; D008875:Middle Aged; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D013454:Sulfoxides; D014508:Urea
chemical_list: D000975:Antioxidants; D015415:Biomarkers; D017879:Isothiocyanates; D010936:Plant Extracts; D013454:Sulfoxides; D008315:Malondialdehyde; D000641:Ammonia; D014508:Urea; C016766:sulforaphane; D005978:Glutathione
keywords: Glutathione; Helicobacter pylori; Malondialdehyde; Sulforaphane