title: Induction of ovulation with buserelin in jennies: in search of the minimum effective dose.
abstract: The aim of this study was to evaluate the minimum dose of buserelin acetate (buserelin) able to induce ovulation between 24 and 48 h from treatment (positive response) in estrous jennies. Jennies were studied during a total of 172 estrous cycles: ovarian activity was routinely monitored by ultrasound; when the dominant follicle reached a diameter of 33 ± 1 mm, estrous jennies were treated by subcutaneous administration of different doses of buserelin, 3.3mg (N = 11), 1.5mg (N = 21), 0.8 mg (N = 12), 0.4 mg (N = 16), 0.2mg (N = 13), 0.1mg (N = 16), 0.04 mg (N = 14), 0.02 mg (N = 16), or employed as controls (N = 53). Single jennies (P = 0.0001) and GnRH dose (P = 0.003) significantly affected ovulation rates. Ovulation rates between 24 and 48 h of each treated group, except for the 0.02 mg group, was higher than in the control group (P < 0.05). The minimum dose of buserelin effective to induce ovulation in estrous jennies was 0.04 mg.
authors.affiliation: Department of Veterinary Sciences, University of Pisa, Via Livornese, San Piero a Grado, 56124 Pisa, Italy. Electronic address: fcamillo@vet.unipi.it.
authors.forename: Francesco
authors.initials: F
authors.lastname: Camillo
authors.affiliation: Department of Veterinary Sciences, University of Pisa, Via Livornese, San Piero a Grado, 56124 Pisa, Italy.
authors.forename: Iacopo
authors.initials: I
authors.lastname: Vannozzi
authors.affiliation: Department of Veterinary Sciences, University of Pisa, Via Livornese, San Piero a Grado, 56124 Pisa, Italy.
authors.forename: Matteo
authors.initials: M
authors.lastname: Tesi
authors.affiliation: Department of Veterinary Sciences, University of Pisa, Via Livornese, San Piero a Grado, 56124 Pisa, Italy.
authors.forename: Chiara
authors.initials: C
authors.lastname: Sabatini
authors.affiliation: Department of Veterinary Sciences, University of Pisa, Via Livornese, San Piero a Grado, 56124 Pisa, Italy.
authors.forename: Alessandra
authors.initials: A
authors.lastname: Rota
authors.affiliation: Department of Veterinary Sciences, University of Pisa, Via Livornese, San Piero a Grado, 56124 Pisa, Italy.
authors.forename: Elisabetta
authors.initials: E
authors.lastname: Paciolla
authors.affiliation: Ecole Nationale Vétérinaire, Agroalimentaire et de l'Alimentation Nantes Atlantique-ONIRIS, 102 Route de Gachet, 44300 Nantes, France.
authors.forename: Irene
authors.initials: I
authors.lastname: Dang-Nguyen
authors.affiliation: Department of Veterinary Sciences, University of Pisa, Via Livornese, San Piero a Grado, 56124 Pisa, Italy.
authors.forename: Duccio
authors.initials: D
authors.lastname: Panzani
mesh_terms: D000818:Animals; D002064:Buserelin / Q000008:administration & dosage / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D018397:Equidae / Q000502:physiology*; D005260:Female; D005300:Fertility Agents, Female / Q000008:administration & dosage / Q000494:pharmacology*; D016015:Logistic Models; D016017:Odds Ratio; D010060:Ovulation / Q000187:drug effects; D010062:Ovulation Induction / Q000662:veterinary*
chemical_list: D005300:Fertility Agents, Female; D002064:Buserelin
keywords: Buserelin; Donkey; GnRH analogs; Induction of ovulation