title: One day to one hour: how quickly can foodborne pathogens be detected?
abstract: Foodborne pathogens pose serious public health risks. Rapid, accurate technologies to detect a low number of target cells (1 cell/25-325 g sample) and microbial toxins are in demand in order to assess product safety in hours to up to 1 day. Varied pathogen loads and the complexity of food present a major challenge. Current culture methods, while accurate, are lengthy. New methods, using brief culturing and detection kits (antibody based, nucleic acid amplification or nano/biosensors) or a culture-independent approach coupled with nucleic acid amplification, traditionally used for viruses/parasites, can be used to obtain results in hours. A strategic approach involving two-step, rapid, high-throughput screening to rule out negatives followed by a confirmatory test could accomplish product testing in 1 h to 1 day.
authors.forename: Arun K
authors.initials: AK
authors.lastname: Bhunia
mesh_terms: D001419:Bacteria / Q000302:isolation & purification*; D015374:Biosensing Techniques / Q000379:methods*; D005516:Food Microbiology / Q000379:methods*; D057166:High-Throughput Screening Assays; D021141:Nucleic Acid Amplification Techniques; D011933:Reagent Kits, Diagnostic; D013997:Time Factors
chemical_list: D011933:Reagent Kits, Diagnostic
keywords: detection; foodborne pathogen; high-throughput screening; rapid