title: Tubeless tracheotomy for survival airway surgery in the leporine model.
abstract: OBJECTIVE
  The ideal animal experimental tracheostomy technique is one that is 1) safe and easy to perform, 2) requires no tracheostomy tube, and 3) requires minimal cleaning or suctioning to maintain patency. The leporine model for airway injury has been well established and offers an inexpensive and practical animal model for experimental evaluation. However, previous research has demonstrated a high mortality rate with survival airway surgery in rabbits. This study demonstrates the feasibility of airway management in the leporine model using a simple maturing suture tracheostomy that avoids a tracheostomy tube.
  
  
  METHODS
  Tracheostomy was performed in six New Zealand white rabbits in the setting of survival surgery over a 2-week study period.
  
  
  METHODS
  A vertical tracheal incision was made from the second to the sixth tracheal ring. The anterior portion of the tracheal rings was removed and the skin surrounding the stoma was sutured down to the tracheal wall. The lateral tracheal wall was then suspended to the soft tissue in the lateral neck.
  
  
  RESULTS
  All six rabbits survived the study period with minimal care and maintained stoma patency until sacrifice. Granulation tissue and edema were noted during the first week and largely resolved by the second week. An average of 5-mm-diameter stoma was measured 14 days after surgery without intraluminal stenosis or laryngeal edema.
  
  
  CONCLUSIONS
  This method meets the defined criteria for the ideal experimental tracheostomy, demonstrating potential benefit in a laryngotracheal stenosis model and a rabbit model of evoked phonation.
authors.affiliation: Department of Otolaryngology-Head & Neck Surgery, Thomas Jefferson University, Philadelphia, Pennsylvania.
authors.forename: Maurits S
authors.initials: MS
authors.lastname: Boon
authors.forename: James J
authors.initials: JJ
authors.lastname: Daniero
authors.forename: Shivam
authors.initials: S
authors.lastname: Saxena
authors.forename: Mark
authors.initials: M
authors.lastname: Balceniuk
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D005500:Follow-Up Studies; D010699:Phonation; D011817:Rabbits; D014135:Tracheal Stenosis / Q000503:physiopathology / Q000601:surgery*; D014140:Tracheotomy / Q000379:methods*; D016896:Treatment Outcome; D014945:Wound Healing
keywords: Tracheostomy; laryngeal stenosis; rabbit model; survival surgery; tracheal stenosis