title: Flavonoid analysis of buckwheat sprouts.
abstract: It is known that common buckwheat sprouts contain several flavonoids, including orientin, isoorientin, vitexin, isovitexin, rutin, and quercetrin, whereas tartary buckwheat sprouts contain only rutin. In this study, we evaluated flavonoids present in buckwheat sprouts and identified a previously unreported flavonoid. Simultaneous detection by HPLC was used to separate rutin and a compound that was not separated in previous studies. We used a novel HPLC elution gradient method to successfully separate rutin and the previously unidentified compound, for which we performed structural analysis. The identification of six flavonoids by HPLC was confirmed using HPLC-ESI-MS/MS analysis. The newly identified compound, [M+H](+) =611.17, was identified by NMR as the rutin epimer quercetin-3-O-robinobioside. Unlike common buckwheat sprout, tartary buckwheat sprout contained rutin as a main flavonoid, whereas other flavonoids appeared only in trace amounts or were not detected. Quercetin-3-O-robinobioside was not detected in tartary buckwheat sprout.
authors.affiliation: Department of Food Science and Technology, College of Life Sciences, Kyung Hee University, Yongin 446-701, Republic of Korea; Graduate School of Biotechnology and Research Institute of Life Sciences & Resources, College of Life Sciences, Kyung Hee University, Yongin 446-701, Republic of Korea.
authors.forename: Tae-Gyu
authors.initials: TG
authors.lastname: Nam
authors.affiliation: Department of Horticultural Biotechnology, College of Life Sciences, Kyung Hee University, Yongin 446-701, Republic of Korea.
authors.forename: Sun Mi
authors.initials: SM
authors.lastname: Lee
authors.affiliation: Graduate School of Biotechnology and Research Institute of Life Sciences & Resources, College of Life Sciences, Kyung Hee University, Yongin 446-701, Republic of Korea.
authors.forename: Ji-Hae
authors.initials: JH
authors.lastname: Park
authors.affiliation: Department of Food Science and Technology, College of Life Sciences, Kyung Hee University, Yongin 446-701, Republic of Korea.
authors.forename: Dae-Ok
authors.initials: DO
authors.lastname: Kim
authors.affiliation: Graduate School of Biotechnology and Research Institute of Life Sciences & Resources, College of Life Sciences, Kyung Hee University, Yongin 446-701, Republic of Korea.
authors.forename: Nam-in
authors.initials: NI
authors.lastname: Baek
authors.affiliation: Department of Horticultural Biotechnology, College of Life Sciences, Kyung Hee University, Yongin 446-701, Republic of Korea. Electronic address: se43@khu.ac.kr.
authors.forename: Seok Hyun
authors.initials: SH
authors.lastname: Eom
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D019612:Fagopyrum / Q000737:chemistry*; D005419:Flavonoids; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D005419:Flavonoids
keywords: Common buckwheat; Quercetin-3-O-robinobioside; Tartary buckwheat; Vegetable sprout