title: Protein carbonylation associated to high-fat, high-sucrose diet and its metabolic effects.
abstract: The present research draws a map of the characteristic carbonylation of proteins in rats fed high-caloric diets with the aim of providing a new insight of the pathogenesis of metabolic diseases derived from the high consumption of fat and refined carbohydrates. Protein carbonylation was analyzed in plasma, liver and skeletal muscle of Sprague-Dawley rats fed a high-fat, high-sucrose (HFHS) diet by a proteomics approach based on carbonyl-specific fluorescence-labeling, gel electrophoresis and mass spectrometry. Oxidized proteins along with specific sites of oxidative damage were identified and discussed to illustrate the consequences of protein oxidation. The results indicated that long-term HFHS consumption increased protein oxidation in plasma and liver; meanwhile, protein carbonyls from skeletal muscle did not change. The increment of carbonylation by HFHS diet was singularly selective on specific target proteins: albumin from plasma and liver, and hepatic proteins such as mitochondrial carbamoyl-phosphate synthase (ammonia), mitochondrial aldehyde dehydrogenase, argininosuccinate synthetase, regucalcin, mitochondrial adenosine triphosphate synthase subunit beta, actin cytoplasmic 1 and mitochondrial glutamate dehydrogenase 1. The possible consequences that these specific protein carbonylations have on the excessive weight gain, insulin resistance and nonalcoholic fatty liver disease resulting from HFHS diet consumption are discussed.
authors.affiliation: Instituto de Investigaciones Marinas, Consejo Superior de Investigaciones Científicas (IIM-CSIC), E-36208 Vigo, Spain. Electronic address: luciamendez@iim.csic.es.
authors.forename: Lucía
authors.initials: L
authors.lastname: Méndez
authors.affiliation: Instituto de Investigaciones Marinas, Consejo Superior de Investigaciones Científicas (IIM-CSIC), E-36208 Vigo, Spain.
authors.forename: Manuel
authors.initials: M
authors.lastname: Pazos
authors.affiliation: Instituto de Química Avanzada de Catalunya, Consejo Superior de Investigaciones Científicas (IQAC-CSIC), E-08034 Barcelona, Spain.
authors.forename: Eunice
authors.initials: E
authors.lastname: Molinar-Toribio
authors.affiliation: Unidad de Farmacología. Facultad de Medicina y Ciencias de la Salud, Universidad Rovira i Virgili, E-43201 Reus, Spain.
authors.forename: Vanesa
authors.initials: V
authors.lastname: Sánchez-Martos
authors.affiliation: Instituto de Investigaciones Marinas, Consejo Superior de Investigaciones Científicas (IIM-CSIC), E-36208 Vigo, Spain.
authors.forename: José M
authors.initials: JM
authors.lastname: Gallardo
authors.affiliation: Unidad de Farmacología. Facultad de Medicina y Ciencias de la Salud, Universidad Rovira i Virgili, E-43201 Reus, Spain.
authors.forename: M
authors.initials: M
authors.lastname: Rosa Nogués
authors.affiliation: Instituto de Química Avanzada de Catalunya, Consejo Superior de Investigaciones Científicas (IQAC-CSIC), E-08034 Barcelona, Spain.
authors.forename: Josep L
authors.initials: JL
authors.lastname: Torres
authors.affiliation: Instituto de Investigaciones Marinas, Consejo Superior de Investigaciones Científicas (IIM-CSIC), E-36208 Vigo, Spain.
authors.forename: Isabel
authors.initials: I
authors.lastname: Medina
mesh_terms: D000444:Aldehyde Dehydrogenase / Q000378:metabolism; D000818:Animals; D001124:Argininosuccinate Synthase / Q000378:metabolism; D002135:Calcium-Binding Proteins / Q000378:metabolism; D002222:Carbamoyl-Phosphate Synthase (Ammonia) / Q000378:metabolism; D002265:Carboxylic Ester Hydrolases; D059305:Diet, High-Fat / Q000009:adverse effects*; D004041:Dietary Fats / Q000008:administration & dosage / Q000009:adverse effects*; D019422:Dietary Sucrose / Q000008:administration & dosage / Q000009:adverse effects*; D007333:Insulin Resistance; D047908:Intracellular Signaling Peptides and Proteins / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008297:Male; D008928:Mitochondria / Q000378:metabolism; D018482:Muscle, Skeletal / Q000378:metabolism; D065626:Non-alcoholic Fatty Liver Disease / Q000503:physiopathology; D050050:Protein Carbonylation; D040901:Proteomics; D051381:Rats; D017207:Rats, Sprague-Dawley; D012709:Serum Albumin / Q000378:metabolism; D015430:Weight Gain
chemical_list: D002135:Calcium-Binding Proteins; D004041:Dietary Fats; D019422:Dietary Sucrose; D047908:Intracellular Signaling Peptides and Proteins; D012709:Serum Albumin; D000444:Aldehyde Dehydrogenase; D002265:Carboxylic Ester Hydrolases; C513325:Rgn protein, rat; D002222:Carbamoyl-Phosphate Synthase (Ammonia); D001124:Argininosuccinate Synthase
keywords: High-fat, high-sucrose diet; Insulin resistance; NAFLD; Obesity; Protein carbonylation; Sprague–Dawley rat