title: Genetic Compositions of Broad bean wilt virus 2 Infecting Red Pepper in Korea.
abstract: The incidence of Broad bean wilt virus 2 (BBWV2) on red pepper was investigated using the samples obtained from 24 areas of 8 provinces in Korea. Two hundred and five samples (79%) out of 260 collected samples were found to be infected with BBWV2. While the single infection rate of BBWV2 was 21.5%, the co-infection rate of BBWV2 with Cucumber mosaic virus, Pepper mottle virus, Pepper mild mottle virus and/or Potato virus Y was 78.5%. To characterize the genetic diversity of BBWV2 Korean isolates, 7 isolates were fully sequenced and analyzed. Phylogenetic analyses revealed that BBWV2 isolates could be divided largely into two groups as Group I and Group II. Based on the partial sequence analyses, 153 selected BBWV2 isolates were subgrouped into GS-I (21.6%), GS-II (3.9%) and GS-III (56.9%). BBWV2 GS-III, which was predominant in Korea, appears to be a new combination between Group I RNA-1 and Group II RNA-2. Viral disease incidence of BBWV2 on red pepper was under 2% before 2004. However, the incidence was increased abruptly to 41.3% in 2005, 58.2% in 2006 and 79% in 2007. These rapid increases might be related with the emergence of new combinations between BBWV2 groups.
authors.affiliation: Crop Protection Division, National Academy of Agricultural Science, Suwon 441-707, Korea ; Department of Plant Medicine, Chungbuk National University, Cheongju 361-763, Korea.
authors.forename: Hae-Ryun
authors.initials: HR
authors.lastname: Kwak
authors.affiliation: Crop Protection Division, National Academy of Agricultural Science, Suwon 441-707, Korea.
authors.forename: Mi-Kyeong
authors.initials: MK
authors.lastname: Kim
authors.affiliation: Crop Protection Division, National Academy of Agricultural Science, Suwon 441-707, Korea.
authors.forename: Moon
authors.initials: M
authors.lastname: Nam
authors.affiliation: Crop Protection Division, National Academy of Agricultural Science, Suwon 441-707, Korea.
authors.forename: Jeong-Soo
authors.initials: JS
authors.lastname: Kim
authors.affiliation: Department of Agricultural Biotechnology, Plant Genomics and Breeding Institute, Seoul National University, Seoul 151-921, Korea.
authors.forename: Kook-Hyung
authors.initials: KH
authors.lastname: Kim
authors.affiliation: Department of Plant Medicine, Chungbuk National University, Cheongju 361-763, Korea.
authors.forename: Byeongjin
authors.initials: B
authors.lastname: Cha
authors.affiliation: Crop Protection Division, National Academy of Agricultural Science, Suwon 441-707, Korea.
authors.forename: Hong-Soo
authors.initials: HS
authors.lastname: Choi
keywords: BBWV2; genetic diversity; red pepper