title: Sustained release of antibiotic complexed by multivalent ion: in vitro and in vivo study for the treatment of peritonitis.
abstract: The main aims of this study are (i) the development of an antibiotic complexed with multivalent ion, which can allow sustained release of the antibiotic without any additional matrix or difficult process and (ii) the feasibility study of the ion-complexed antibiotic as a therapeutic technique for peritonitis treatment. An ion-complexed antibiotic is prepared by simple mixing of two aqueous solutions containing an ionized (water-soluble) drug (tetracycline) and a multivalent counter ionic compound. The ion-complexed antibiotic shows a continuous release of the antibiotic up to 21 days, and thus prolonged anti-bacterial effect by gradual ionic exchange between the multivalent ions in the complex and same-charged monovalent ions in surrounding medium. From the in vivo animal study using a cecum perforated peritonitis mouse model, the ion-complexed antibiotic group shows sufficient anti-bacterial effect and thus effectively treat the peritonitis because of the extermination of the contaminated enteric bacteria in the peritoneum during wound healing of injury cecum (by the sustained release of antibiotic from the ion complex). These results suggest that the ion-complexed antibiotic system may be promising for the effective treatment of the peritonitis caused by frequent gastrointestinal defect in clinical fields.
authors.affiliation: Department of Advanced Materials, Hannam University, Daejeon 305-811, Republic of Korea.
authors.forename: Seung Yeon
authors.initials: SY
authors.lastname: Na
authors.affiliation: Department of Nanobiomedical Science & BK21 PLUS NBM Global Research Center for Regenerative Medicine, Dankook University, Cheonan 330-714, Republic of Korea.
authors.forename: Se Heang
authors.initials: SH
authors.lastname: Oh
authors.affiliation: Department of Advanced Materials, Hannam University, Daejeon 305-811, Republic of Korea.
authors.forename: Tae Ho
authors.initials: TH
authors.lastname: Kim
authors.affiliation: Department of Biological Sciences and Biotechnology, Hannam University, Daejeon 305-811, Republic of Korea.
authors.forename: Jin A
authors.initials: JA
authors.lastname: Yoon
authors.affiliation: Department of Biological Sciences and Biotechnology, Hannam University, Daejeon 305-811, Republic of Korea.
authors.forename: In Soo
authors.initials: IS
authors.lastname: Lee
authors.affiliation: Department of Advanced Materials, Hannam University, Daejeon 305-811, Republic of Korea. Electronic address: jhlee@hnu.kr.
authors.forename: Jin Ho
authors.initials: JH
authors.lastname: Lee
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage* / Q000737:chemistry / Q000494:pharmacology; D003692:Delayed-Action Preparations; D004195:Disease Models, Animal; D005240:Feasibility Studies; D007474:Ion Exchange; D007477:Ions / Q000737:chemistry*; D051379:Mice; D008810:Mice, Inbred C57BL; D041681:NIH 3T3 Cells; D010538:Peritonitis / Q000188:drug therapy*; D013752:Tetracycline / Q000008:administration & dosage* / Q000737:chemistry / Q000494:pharmacology; D013997:Time Factors
chemical_list: D000900:Anti-Bacterial Agents; D003692:Delayed-Action Preparations; D007477:Ions; D013752:Tetracycline
keywords: Antibiotic; Ion complex; Peritonitis; Sodium phosphate dibasic (PubChem CID: 24203); Sustained release; Tetracycline hydrochloride (PubChem CID: 54704426)