title: Bathysa cuspidata extract modulates the morphological reorganization of the scar tissue and accelerates skin wound healing in rats: a time-dependent study.
abstract: The technological development of pharmaceutical products based on plant extracts is currently responsible for a large number of recent innovations in healthcare. The objective of this study was to develop and investigate the effect and potential applicability of an ointment-based Bathysa cuspidata extract (BCE) for the management of skin wounds in rats. Three skin wounds of 12 mm in diameter were made on the backs of the animals, which were randomized into 4 groups according to the application received, i.e. the SAL group: 0.9% saline solution, the LAN group: lanolin, the BCE 2.5% group: 2.5% BCE emulsified in lanolin and the BCE 5% group: 5% BCE emulsified in lanolin. The applications were made daily over 21 days, and every 7 days tissue from different wounds was removed. On days 7, 14 and 21, the BCE 2.5% and BCE 5% groups showed the best results in relation to wound closure, and a higher proportion (in length, density and volume) of blood vessels and fibroblasts compared to the other groups. On days 7 and 14, there was a significant increase in the number of mast cells in these 2 groups when compared to the SAL and LAN groups. On day 21, they also had a higher proportion of collagen I than collagen III. B. cuspidata in an ointment base was effective in stimulating tissue cellularity, mast cell recruitment, neoangiogenesis, synthesis and maturation of collagen, epidermal thickness and surface area in scar tissue. These events were potentially related to the best quality and speed for skin regeneration in the rats treated with the BCE ointment.
authors.affiliation: Department of Animal Biology, Federal University of Viçosa, Viçosa, Brazil.
authors.forename: Reggiani V
authors.initials: RV
authors.lastname: Gonçalves
authors.forename: Rômulo D
authors.initials: RD
authors.lastname: Novaes
authors.forename: Marli C
authors.initials: MC
authors.lastname: Cupertino
authors.forename: Bruna M
authors.initials: BM
authors.lastname: Araújo
authors.forename: Emerson F
authors.initials: EF
authors.lastname: Vilela
authors.forename: Aline T
authors.initials: AT
authors.lastname: Machado
authors.forename: João P V
authors.initials: JP
authors.lastname: Leite
authors.forename: Sérgio L P
authors.initials: SL
authors.lastname: Matta
mesh_terms: D000818:Animals; D003094:Collagen / Q000378:metabolism*; D048091:Guided Tissue Regeneration / Q000379:methods*; D008517:Phytotherapy / Q000379:methods*; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use*; D051381:Rats; D017208:Rats, Wistar; D012867:Skin / Q000293:injuries*; D013997:Time Factors; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D010936:Plant Extracts; D003094:Collagen