title: Abi3bp regulates cardiac progenitor cell proliferation and differentiation.
abstract: BACKGROUND
  Cardiac progenitor cells (CPCs) are thought to differentiate into the major cell types of the heart: cardiomyocytes, smooth muscle cells, and endothelial cells. We have recently identified ABI family, member 3 (NESH) binding protein (Abi3bp) as a protein important for mesenchymal stem cell biology. Because CPCs share several characteristics with mesenchymal stem cells, we hypothesized that Abi3bp would similarly affect CPC differentiation and proliferation.
  
  
  OBJECTIVE
  To determine whether Abi3bp regulates CPC proliferation and differentiation.
  
  
  RESULTS
  In vivo, genetic ablation of the Abi3bp gene inhibited CPC differentiation, whereas CPC number and proliferative capacity were increased. This correlated with adverse recovery after myocardial infarction. In vitro, CPCs, either isolated from Abi3bp knockout mice or expressing an Abi3bp shRNA construct, displayed a higher proliferative capacity and, under differentiating conditions, reduced expression of both early and late cardiomyocyte markers. Abi3bp controlled CPC differentiation via integrin-β1, protein kinase C-ζ, and v-akt murine thymoma viral oncogene homolog.
  
  
  CONCLUSIONS
  We have identified Abi3bp as a protein important for CPC differentiation and proliferation.
authors.affiliation: From the Mandel Center for Hypertension Research and Division of Cardiovascular Medicine, Department of Medicine, Duke University Medical Center, Durham, NC.
authors.forename: Conrad P
authors.initials: CP
authors.lastname: Hodgkinson
authors.affiliation: From the Mandel Center for Hypertension Research and Division of Cardiovascular Medicine, Department of Medicine, Duke University Medical Center, Durham, NC.
authors.forename: Jose A
authors.initials: JA
authors.lastname: Gomez
authors.affiliation: From the Mandel Center for Hypertension Research and Division of Cardiovascular Medicine, Department of Medicine, Duke University Medical Center, Durham, NC.
authors.forename: Alan J
authors.initials: AJ
authors.lastname: Payne
authors.affiliation: From the Mandel Center for Hypertension Research and Division of Cardiovascular Medicine, Department of Medicine, Duke University Medical Center, Durham, NC.
authors.forename: Lunan
authors.initials: L
authors.lastname: Zhang
authors.affiliation: From the Mandel Center for Hypertension Research and Division of Cardiovascular Medicine, Department of Medicine, Duke University Medical Center, Durham, NC.
authors.forename: Xiaowen
authors.initials: X
authors.lastname: Wang
authors.affiliation: From the Mandel Center for Hypertension Research and Division of Cardiovascular Medicine, Department of Medicine, Duke University Medical Center, Durham, NC.
authors.forename: Sophie
authors.initials: S
authors.lastname: Dal-Pra
authors.affiliation: From the Mandel Center for Hypertension Research and Division of Cardiovascular Medicine, Department of Medicine, Duke University Medical Center, Durham, NC.
authors.forename: Richard E
authors.initials: RE
authors.lastname: Pratt
authors.affiliation: From the Mandel Center for Hypertension Research and Division of Cardiovascular Medicine, Department of Medicine, Duke University Medical Center, Durham, NC. victor.dzau@duke.edu.
authors.forename: Victor J
authors.initials: VJ
authors.lastname: Dzau
mesh_terms: D000818:Animals; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D004195:Disease Models, Animal; D019012:Integrin beta1 / Q000378:metabolism; D007527:Isoenzymes / Q000378:metabolism; D008297:Male; D059630:Mesenchymal Stem Cells / Q000378:metabolism* / Q000473:pathology; D018345:Mice, Knockout; D009200:Myocardial Contraction; D009203:Myocardial Infarction / Q000235:genetics / Q000378:metabolism* / Q000473:pathology / Q000503:physiopathology; D032383:Myocytes, Cardiac / Q000378:metabolism* / Q000473:pathology; D011493:Protein Kinase C / Q000378:metabolism; D000074121:Protein Kinase C-theta; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D034622:RNA Interference; D020127:Recovery of Function; D012038:Regeneration; D015398:Signal Transduction; D013318:Stroke Volume; D013997:Time Factors; D014162:Transfection
chemical_list: D002352:Carrier Proteins; D019012:Integrin beta1; D007527:Isoenzymes; C527603:TARSH protein, mouse; D051057:Proto-Oncogene Proteins c-akt; C489593:Prkcq protein, mouse; D011493:Protein Kinase C; D000074121:Protein Kinase C-theta
keywords: extracellular matrix; integrin-β