title: Development of a Selective Medium for the Fungal Pathogen Fusarium graminearum Using Toxoflavin Produced by the Bacterial Pathogen Burkholderia glumae.
abstract: The ascomycete fungus Fusarium graminearum is a major causal agent for Fusarium head blight in cereals and produces mycotoxins such as trichothecenes and zearalenone. Isolation of the fungal strains from air or cereals can be hampered by various other airborne fungal pathogens and saprophytic fungi. In this study, we developed a selective medium specific to F. graminearum using toxoflavin produced by the bacterial pathogen Burkholderia glumae. F. graminearum was resistant to toxoflavin, while other fungi were sensitive to this toxin. Supplementing toxoflavin into medium enhanced the isolation of F. graminearum from rice grains by suppressing the growth of saprophytic fungal species. In addition, a medium with or without toxoflavin exposed to wheat fields for 1 h had 84% or 25%, respectively, of colonies identified as F. graminearum. This selection medium provides an efficient tool for isolating F. graminearum, and can be adopted by research groups working on genetics and disease forecasting.
authors.affiliation: Department of Applied Biology, Dong-A University, Busan 604-714, Korea.
authors.forename: Boknam
authors.initials: B
authors.lastname: Jung
authors.affiliation: Department of Applied Biology, Dong-A University, Busan 604-714, Korea.
authors.forename: Sehee
authors.initials: S
authors.lastname: Lee
authors.affiliation: Department of Applied Biology, Dong-A University, Busan 604-714, Korea.
authors.forename: Jiran
authors.initials: J
authors.lastname: Ha
authors.affiliation: Department of Rice and Winter Cereal Crop, National Institute of Crop Science, Rural Development Administration, Iksan 570-080, Korea.
authors.forename: Jong-Chul
authors.initials: JC
authors.lastname: Park
authors.affiliation: Crop Environment Division, National Institute of Crop Science, Rural Development Administration, Suwon 441-857, Korea.
authors.forename: Sung-Sook
authors.initials: SS
authors.lastname: Han
authors.affiliation: Department of Agricultural Biotechnology, Seoul National University, Seoul 151-921, Korea.
authors.forename: Ingyu
authors.initials: I
authors.lastname: Hwang
authors.affiliation: Department of Agricultural Biotechnology, Seoul National University, Seoul 151-921, Korea.
authors.forename: Yin-Won
authors.initials: YW
authors.lastname: Lee
authors.affiliation: Department of Applied Biology, Dong-A University, Busan 604-714, Korea.
authors.forename: Jungkwan
authors.initials: J
authors.lastname: Lee
keywords: Burkholderia; Fusarium; Fusarium head blight; selective medium; toxoflavin