title: Protective effect of delta opioid agonist [D-Ala2, D-Leu5] enkephalin on spinal cord ischemia reperfusion injury by regional perfusion into abdominal aorta in rabbits.
abstract: [D-Ala(2), D-Leu(5)] enkephalin (DADLE) has been reported to exhibit protective effects against hypoxic or ischemic induced brain insult. However its efficacy on the spinal cord ischemia-reperfusion injury remains unclear. Here we investigate whether DADLE could attenuate ischemia and reperfusion induced neural injury in the rabbit spinal cord. New Zealand white rabbits were subjected to spinal cord ischemia by infrarenal aortic occlusion for 30 min. In the period of spinal cord ischemia, DADLE 0.5 mg/kg or NS were infused continuously into the distal clamped abdominal aorta. The heart rate, blood pressure, and core temperature were monitored continuously during the whole experimental procedure. Then the neurological behavioral function was assessed with Tarlov scale system at 1h, 6h, 24h, 48 h after reperfusion, and neuronal injury evaluation in the ventral horn of gray matter was measured by counting the normal motor neurons at 48 h after reperfusion. Comparing with the control group, the Tarlov scores were significantly higher and the incidences of paraplegia were significantly lower in the DADLE group at four time-point recorded. In addition, the normal neurons numbers in the DADLE group were significant more than those in the control group at 48 h after reperfusion. These results suggested that DADLE infused into the abdominal aorta during ischemia period could attenuate behavioral retardation and the loss of normal motor neuron induced by ischemia-reperfusion in rabbits.
authors.affiliation: Department of Anesthesiology, Shanghai First People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200080, China.
authors.forename: Haitong
authors.initials: H
authors.lastname: Liu
authors.affiliation: Department of Anesthesiology, Shanghai First People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200080, China.
authors.forename: Binbin
authors.initials: B
authors.lastname: Chen
authors.affiliation: Department of Anesthesiology, Shanghai First People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200080, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Anesthesiology, Shanghai First People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200080, China.
authors.forename: Yimin
authors.initials: Y
authors.lastname: Qiu
authors.affiliation: Department of Anesthesiology, Shanghai First People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200080, China.
authors.forename: Yunfei
authors.initials: Y
authors.lastname: Xia
authors.affiliation: Department of Anesthesiology, Shanghai First People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200080, China.
authors.forename: Shitong
authors.initials: S
authors.lastname: Li
authors.affiliation: Department of Anesthesiology, Shanghai First People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200080, China. Electronic address: sunshineyao@163.com.
authors.forename: Junyan
authors.initials: J
authors.lastname: Yao
mesh_terms: D000818:Animals; D001012:Aorta, Abdominal; D016308:Enkephalin, Leucine-2-Alanine / Q000008:administration & dosage / Q000627:therapeutic use*; D005260:Female; D006439:Hemodynamics / Q000187:drug effects; D008297:Male; D009474:Neurons / Q000187:drug effects / Q000473:pathology; D018696:Neuroprotective Agents / Q000008:administration & dosage / Q000627:therapeutic use*; D010477:Perfusion; D011817:Rabbits; D017465:Receptors, Opioid, delta / Q000819:agonists*; D015427:Reperfusion Injury / Q000473:pathology / Q000503:physiopathology / Q000517:prevention & control*; D020760:Spinal Cord Ischemia / Q000473:pathology / Q000503:physiopathology / Q000517:prevention & control*
chemical_list: D018696:Neuroprotective Agents; D017465:Receptors, Opioid, delta; D016308:Enkephalin, Leucine-2-Alanine
keywords: DADLE; Ischemia reperfusion injury; Neuroprotection; Rabbits; Spinal cord