title: Development and Practical Use of RT-PCR for Seed-transmitted Prune dwarf virus in Quarantine.
abstract: Among imported plants, seeds are the items that have many latent pathogens and are difficult to inspect. Also, they are the import and export items whose market is expected to expand. The biggest problem with seeds is viruses. Prune dwarf virus (PDV) is the virus that is commonly inspected in Prunus cerasifera, P. persica, P. armeniaca, P. mandshurica, P. cerasus, P. avium or P. serotina seeds. In this study, two RT-PCR primer sets, which can promptly and specifically diagnose plant quarantine seed-transmitted PDV, were developed; and nested PCR primers, where products amplify 739 and 673 nucleotides (nt), and an nested PCR-product, 305 nt, can be obtained as these products are amplified again, were developed. Also, a modified-positive control plasmid was developed, where the restriction enzyme XhoI, which can identify the contamination of samples from the control, was inserted. The method developed in this study has detected PDV in 18 cases since 2007, and is expected to continuously contribute to the plant quarantine in Korea.
authors.affiliation: Water Supply & Sewerage Research Division, National Institute of Environmental Research, Incheon 440-170, Korea.
authors.forename: Siwon
authors.initials: S
authors.lastname: Lee
authors.affiliation: Incheon International Airport Regional Office, Animal and Plant Quarantine Agency, Incheon 400-044, Korea.
authors.forename: Yong-Gil
authors.initials: YG
authors.lastname: Shin
keywords: Prune dwarf virus; RT-PCR; nested PCR; quarantine; seed-transmitted