title: Roles of the co-culture of human umbilical cord Wharton's jelly-derived mesenchymal stem cells with rat pancreatic cells in the treatment of rats with diabetes mellitus.
abstract: The aim of the present study was to investigate the roles of the co-culture of human umbilical cord Wharton's jelly-derived mesenchymal stem cells (hUC-MSCs) with rat pancreatic cells in the treatment of rats with diabetes mellitus. hUC-MSCs were isolated and passaged, followed by Transwell co-culture with rat pancreatic cells. The induced islet-like cell clusters were transplanted into the renal capsule in rats with streptozotocin (STZ)-induced diabetes mellitus. The effects of co-culture on blood glucose levels in rats were observed. The isolated hUC-MSCs expressed the specific surface markers, including cluster of differentiation 44 (CD44) (91.4%), CD29 (91.3%) and CD105 (99.2%). Following co-culture with hUC-MSCs for 7 and 10 days, the rat pancreatic cells were strongly stained by pancreatic and duodenal homeobox-1 and human insulin. The insulin and C-peptide concentrations were increased significantly compared to the pure culture group. One week following the transplantation of induced islet-like cells into the renal capsule, the blood glucose level of rats in the STZ experimental group was significantly lower than that of the STZ control group. There were notable 5-bromo-2'-deoxyuridine-positive nuclei and insulin-positive cytoplasm in the renal capsule following cell transplantation. Therefore, co-culture of hUC-MSCs with rat pancreatic cells can lower the blood glucose levels in rats with diabetes mellitus.
authors.affiliation: Ulcerous Vascular Surgical Department, Beijing TCM Hospital Affiliated to Capital Medical University, Beijing 100010, P.R. China.
authors.forename: Guangyu
authors.initials: G
authors.lastname: Wang
authors.affiliation: Ulcerous Vascular Surgical Department, Beijing TCM Hospital Affiliated to Capital Medical University, Beijing 100010, P.R. China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Li
authors.affiliation: Ulcerous Vascular Surgical Department, Beijing TCM Hospital Affiliated to Capital Medical University, Beijing 100010, P.R. China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Ulcerous Vascular Surgical Department, Beijing TCM Hospital Affiliated to Capital Medical University, Beijing 100010, P.R. China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Dong
authors.affiliation: Ulcerous Vascular Surgical Department, Beijing TCM Hospital Affiliated to Capital Medical University, Beijing 100010, P.R. China.
authors.forename: Fu-Sheng
authors.initials: FS
authors.lastname: Wang
authors.affiliation: Ulcerous Vascular Surgical Department, Beijing TCM Hospital Affiliated to Capital Medical University, Beijing 100010, P.R. China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Ding
authors.affiliation: Ulcerous Vascular Surgical Department, Beijing TCM Hospital Affiliated to Capital Medical University, Beijing 100010, P.R. China.
authors.forename: Yudong
authors.initials: Y
authors.lastname: Kang
authors.affiliation: Ulcerous Vascular Surgical Department, Beijing TCM Hospital Affiliated to Capital Medical University, Beijing 100010, P.R. China.
authors.forename: Xuying
authors.initials: X
authors.lastname: Xu
keywords: Wharton’s jelly; diabetes mellitus; mesenchymal stem cells; transplantation; umbilical cord