title: Hydrogen peroxide biosensor based on microperoxidase-11 immobilized on flexible MWCNTs-BC nanocomposite film.
abstract: In the present work, we report on an experimental study of flexible nanocomposite film for electrochemical detection of hydrogen peroxide (H2O2) based on bacterial cellulose (BC) and multi-walled carbon nanotubes (MWCNTs) in combination with microperoxidase-11 (MP-11). MWCNTs are used to functionalize BC and provide a flexible conductive film. On the other hand, BC can improve MWCNTs׳ biocompatibility. The investigation shows that MP-11 immobilized on the flexible film of MWCNTs-BC can easily present a pair of well-defined and quasi-reversible redox peaks, revealing a direct electrochemistry of MP-11 on the nanocomposite film. The apparent heterogeneous electron-transfer rate constant ks is estimated to be 11.5s(-1). The resulting flexible electrode presents appreciated catalytic properties for electrochemical detection of H2O2, comparing to traditional electrodes (such as gold, glassy carbon electrode) modified with MP-11. The proposed biosensor exhibits a low detection limit of 0.1 µM (at a signal-to-noise ratio of 3) with a linear range of 0.1-257.6 µM, and acquires a satisfactory stability.
authors.affiliation: Wuhan National Laboratory for Optoelectrosnics, School of Optoelectronic Science and Engineering, Huazhong University of Science and Technology, Wuhan 430074, P.R. China.
authors.forename: Bingyan
authors.initials: B
authors.lastname: Zhang
authors.affiliation: National Engineering Research Center for Nano-Medicine, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, 430074, P.R. China.
authors.forename: Jianhai
authors.initials: J
authors.lastname: Zhou
authors.affiliation: Wuhan National Laboratory for Optoelectrosnics, School of Optoelectronic Science and Engineering, Huazhong University of Science and Technology, Wuhan 430074, P.R. China.
authors.forename: Shaohui
authors.initials: S
authors.lastname: Li
authors.affiliation: Wuhan National Laboratory for Optoelectrosnics, School of Optoelectronic Science and Engineering, Huazhong University of Science and Technology, Wuhan 430074, P.R. China.
authors.forename: Xiaofan
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Wuhan National Laboratory for Optoelectrosnics, School of Optoelectronic Science and Engineering, Huazhong University of Science and Technology, Wuhan 430074, P.R. China.
authors.forename: Dekang
authors.initials: D
authors.lastname: Huang
authors.affiliation: Wuhan National Laboratory for Optoelectrosnics, School of Optoelectronic Science and Engineering, Huazhong University of Science and Technology, Wuhan 430074, P.R. China.
authors.forename: Yahui
authors.initials: Y
authors.lastname: He
authors.affiliation: Wuhan National Laboratory for Optoelectrosnics, School of Optoelectronic Science and Engineering, Huazhong University of Science and Technology, Wuhan 430074, P.R. China.
authors.forename: Mingkui
authors.initials: M
authors.lastname: Wang
authors.affiliation: National Engineering Research Center for Nano-Medicine, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, 430074, P.R. China. Electronic address: yang_sunny@yahoo.com.
authors.forename: Guang
authors.initials: G
authors.lastname: Yang
authors.affiliation: Wuhan National Laboratory for Optoelectrosnics, School of Optoelectronic Science and Engineering, Huazhong University of Science and Technology, Wuhan 430074, P.R. China. Electronic address: ciac_sheny@mail.hust.edu.cn.
authors.forename: Yan
authors.initials: Y
authors.lastname: Shen
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D002384:Catalysis; D002482:Cellulose / Q000737:chemistry / Q000378:metabolism*; D004563:Electrochemistry; D004566:Electrodes; D006861:Hydrogen Peroxide / Q000032:analysis*; D057230:Limit of Detection; D053761:Nanocomposites / Q000737:chemistry*; D037742:Nanotubes, Carbon / Q000737:chemistry*; D010544:Peroxidases / Q000737:chemistry / Q000378:metabolism*
chemical_list: D037742:Nanotubes, Carbon; D002482:Cellulose; D006861:Hydrogen Peroxide; D010544:Peroxidases; C021799:microperoxidase
keywords: Bacterial cellulose; Biosensor; H(2)O(2) detection; Microperoxidase-11; Multi-walled carbon nanotubes