title: MicroRNA-874 inhibits cell proliferation and induces apoptosis in human breast cancer by targeting CDK9.
abstract: It has been demonstrated that miR-874 plays important roles in many types of cancers. Nevertheless, its biological function in breast cancer remains largely unknown. In this study, we found that the expression level of miR-874 is down-regulated in breast cancer in comparison with the adjacent normal tissues. The overexpression of miR-874 is able to inhibit cell proliferation and induce cell apoptosis and cell cycle arrest in MCF7 and MDA-MB-231 cells. Using a bioinformatics method, we further show that CDK9 is a direct target of miR-874 and that its protein level is negatively regulated by miR-874. Therefore, the data reported in this manuscript demonstrate that miR-874 is an important regulator in breast cancer and imply that the miR-874/CDK9 axis has potential as a therapeutic target for breast cancer.
authors.affiliation: Analytical & Testing Center, Nanjing Medical University, Nanjing, Jiangsu 210029, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Wang
authors.affiliation: Department of Oncology, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China.
authors.forename: Wen
authors.initials: W
authors.lastname: Gao
authors.affiliation: Analytical & Testing Center, Nanjing Medical University, Nanjing, Jiangsu 210029, China.
authors.forename: Fan
authors.initials: F
authors.lastname: Hu
authors.affiliation: Analytical & Testing Center, Nanjing Medical University, Nanjing, Jiangsu 210029, China.
authors.forename: Zhiyang
authors.initials: Z
authors.lastname: Xu
authors.affiliation: Analytical & Testing Center, Nanjing Medical University, Nanjing, Jiangsu 210029, China. Electronic address: wangfuqiang0802@163.com.
authors.forename: Fuqiang
authors.initials: F
authors.lastname: Wang
mesh_terms: D000328:Adult; D000368:Aged; D017209:Apoptosis / Q000235:genetics*; D001483:Base Sequence; D001943:Breast Neoplasms / Q000235:genetics* / Q000473:pathology*; D002453:Cell Cycle / Q000235:genetics; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics; D042863:Cyclin-Dependent Kinase 9 / Q000172:deficiency / Q000235:genetics*; D015536:Down-Regulation / Q000235:genetics; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D055785:Gene Knockdown Techniques; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged
chemical_list: C561946:MIRN874 microRNA, human; D035683:MicroRNAs; D042863:Cyclin-Dependent Kinase 9
keywords: Apoptosis; Breast cancer; CDK9; Cell cycle; Cell proliferation; miR-874