title: Epithelial cell morphology and adhesion on diamond films deposited and chemically modified by plasma processes.
abstract: The authors show that nanocrystalline diamond (NCD) thin films prepared by microwave plasma enhanced chemical vapor deposition apparatus with a linear antenna delivery system are well compatible with epithelial cells (5637 human bladder carcinoma) and significantly improve the cell adhesion compared to reference glass substrates. This is attributed to better adhesion of adsorbed layers to diamond as observed by atomic force microscopy (AFM) beneath the cells. Moreover, the cell morphology can be adjusted by appropriate surface treatment of diamond by using hydrogen and oxygen plasma. Cell bodies, cytoplasmic rims, and filopodia were characterized by Peakforce AFM. Oxidized NCD films perform better than other substrates under all conditions (96% of cells adhered well). A thin adsorbed layer formed from culture medium and supplemented with fetal bovine serum (FBS) covered the diamond surface and played an important role in the cell adhesion. Nevertheless, 50-100 nm large aggregates formed from the RPMI medium without FBS facilitated cell adhesion also on hydrophobic hydrogenated NCD (increase from 23% to 61%). The authors discuss applicability for biomedical uses.
authors.affiliation: Department of Thin Films and Nanostructures, Institute of Physics ASCR, Cukrovarnicka 10, 16200 Praha 6, Czech Republic.
authors.forename: Bohuslav
authors.initials: B
authors.lastname: Rezek
authors.affiliation: Department of Thin Films and Nanostructures, Institute of Physics ASCR, Cukrovarnicka 10, 16200 Praha 6, Czech Republic.
authors.forename: Egor
authors.initials: E
authors.lastname: Ukraintsev
authors.affiliation: Department of Thin Films and Nanostructures, Institute of Physics ASCR, Cukrovarnicka 10, 16200 Praha 6, Czech Republic.
authors.forename: Marie
authors.initials: M
authors.lastname: Krátká
authors.affiliation: Department of Functional Materials, Institute of Physics ASCR, Na Slovance 2, 18221 Praha 8, Czech Republic.
authors.forename: Andrew
authors.initials: A
authors.lastname: Taylor
authors.affiliation: Department of Functional Materials, Institute of Physics ASCR, Na Slovance 2, 18221 Praha 8, Czech Republic.
authors.forename: Frantisek
authors.initials: F
authors.lastname: Fendrych
authors.affiliation: Department of Pathology, Third Faculty of Medicine, Charles University in Prague, Ruská 87, 10000 Praha 10, Czech Republic.
authors.forename: Vaclav
authors.initials: V
authors.lastname: Mandys
mesh_terms: D002448:Cell Adhesion; D045744:Cell Line, Tumor; D048430:Cell Shape; D018130:Diamond / Q000737:chemistry*; D004847:Epithelial Cells / Q000166:cytology* / Q000502:physiology* / Q000648:ultrastructure; D006801:Humans; D018625:Microscopy, Atomic Force; D049329:Nanostructures / Q000737:chemistry*
chemical_list: D018130:Diamond