title: Uniaxially aligned electrospun cellulose acetate nanofibers for thin layer chromatographic screening of hydroquinone and retinoic acid adulterated in cosmetics.
abstract: Uniaxially aligned cellulose acetate (CA) nanofibers were successfully fabricated by electrospinning and applied to use as stationary phase for thin layer chromatography. The control of alignment was achieved by using a drum collector rotating at a high speed of 6000 rpm. Spin time of 6h was used to produce the fiber thickness of about 10 μm which was adequate for good separation. Without any chemical modification after the electrospinning process, CA nanofibers could be readily devised for screening hydroquinone (HQ) and retinoic acid (RA) adulterated in cosmetics using the mobile phase consisting of 65:35:2.5 methanol/water/acetic acid. It was found that the separation run on the aligned nanofibers over a distance of 5 cm took less than 15 min which was two to three times faster than that on the non-aligned ones. On the aligned nanofibers, the masses of HQ and RA which could be visualized were 10 and 25 ng, respectively, which were two times lower than those on the non-aligned CA fibers and five times lower than those on conventional silica plates due to the appearance of darker and sharper of spots on the aligned nanofibers. Furthermore, the proposed method efficiently resolved HQ from RA and ingredients commonly found in cosmetic creams. Due to the satisfactory analytical performance, facile and inexpensive production process, uniaxially aligned electrospun CA nanofibers are promising alternative media for planar chromatography.
authors.affiliation: Pharmaceutical Development of Green Innovations Group (PDGIG), Faculty of Pharmacy, Silpakorn University, Nakhon Pathom 73000, Thailand.
authors.forename: Siripran
authors.initials: S
authors.lastname: Tidjarat
authors.affiliation: Pharmaceutical Development of Green Innovations Group (PDGIG), Faculty of Pharmacy, Silpakorn University, Nakhon Pathom 73000, Thailand.
authors.forename: Weerapath
authors.initials: W
authors.lastname: Winotapun
authors.affiliation: Pharmaceutical Development of Green Innovations Group (PDGIG), Faculty of Pharmacy, Silpakorn University, Nakhon Pathom 73000, Thailand.
authors.forename: Praneet
authors.initials: P
authors.lastname: Opanasopit
authors.affiliation: Pharmaceutical Development of Green Innovations Group (PDGIG), Faculty of Pharmacy, Silpakorn University, Nakhon Pathom 73000, Thailand.
authors.forename: Tanasait
authors.initials: T
authors.lastname: Ngawhirunpat
authors.affiliation: Pharmaceutical Development of Green Innovations Group (PDGIG), Faculty of Pharmacy, Silpakorn University, Nakhon Pathom 73000, Thailand. Electronic address: rtheerasak@yahoo.com.
authors.forename: Theerasak
authors.initials: T
authors.lastname: Rojanarata
mesh_terms: D002482:Cellulose / Q000031:analogs & derivatives* / Q000737:chemistry; D002855:Chromatography, Thin Layer / Q000295:instrumentation* / Q000379:methods; D003358:Cosmetics / Q000737:chemistry*; D006873:Hydroquinones / Q000032:analysis*; D057139:Nanofibers / Q000737:chemistry*; D012822:Silicon Dioxide; D014212:Tretinoin / Q000032:analysis*
chemical_list: D003358:Cosmetics; D006873:Hydroquinones; C005062:acetylcellulose; D014212:Tretinoin; D012822:Silicon Dioxide; D002482:Cellulose; C031927:hydroquinone
keywords: Cellulose acetate; Electrospinning; Nanofibers; TLC; Uniaxially aligned