title: Clinical application of immunomagnetic reduction for quantitative measurement of insulin-like growth factor binding protein-1 in the prediction of pregnant women with preterm premature rupture of membranes.
abstract: BACKGROUND
  Insulin-like growth factor binding protein-1 (IGFBP-1) constitutes a subgroup of the insulin-like growth factor binding protein systems, and its concentration in amniotic fluid is 100-1000 times higher than the concentration in other body fluids. The aim of this study was to evaluate the clinical application of a novel method immunomagnetic reduction (IMR) for quantitative measurement of IGFBP-1 concentrations in the cervicovaginal secretions to diagnose pregnant women with preterm premature rupture of membranes (PPROM).
  
  
  METHODS
  We established a standard calibration curve of IMR intensity against IGFBP-1 concentration based on standard IGFBP-1 samples. We used the IMR assay to detect IGFBP-1 concentrations in the cervicovaginal secretions of pregnant women which were divided into two groups according to the presence or absence of PPROM.
  
  
  RESULTS
  The calibration curve extended from 0.1ng/mL to 10000ng/mL with an excellent correlation (R(2)=0.999). Twenty-two pregnant women between 22 and 34weeks of gestation were analyzed in this prospective study, of whom 10 were clinical evidence of PPROM, and 12 were intact membranes. Through the analysis of receiver-operating characteristic curve, the cut-off point for IMR to differentiate intact membranes from PPROM is 1.015%, which resulted in 90.0, 83.3, 81.8, and 90.9% for sensitivity, specificity, positive predictive value, and negative predictive value, respectively.
  
  
  CONCLUSIONS
  It is evidenced that IMR assay can quantitatively analyze IGFBP-1 concentrations, and the results show the possibility to diagnose pregnant women with PPROM by IMR assay.
authors.affiliation: Institute of Biomedical Engineering, National Taiwan University, Taipei, Taiwan; Department of Obstetrics and Gynecology, Mackay Memorial Hospital, Taipei, Taiwan; Mackay Junior College of Medicine, Nursing, and Management, Taipei, Taiwan; Mackay Medical College, Taipei, Taiwan.
authors.forename: Chen-Yu
authors.initials: CY
authors.lastname: Chen
authors.affiliation: Institute of Biomedical Engineering, National Taiwan University, Taipei, Taiwan.
authors.forename: Chia-Chen
authors.initials: CC
authors.lastname: Chang
authors.affiliation: Institute of Biomedical Engineering, National Taiwan University, Taipei, Taiwan. Electronic address: cwlinx@ntu.edu.tw.
authors.forename: Chii-Wann
authors.initials: CW
authors.lastname: Lin
mesh_terms: D000328:Adult; D000653:Amniotic Fluid / Q000737:chemistry*; D015415:Biomarkers / Q000378:metabolism; D002138:Calibration; D002584:Cervix Uteri / Q000737:chemistry; D005260:Female; D005322:Premature Rupture of Fetal Membranes / Q000175:diagnosis* / Q000378:metabolism* / Q000473:pathology; D005865:Gestational Age; D006801:Humans; D007118:Immunoassay; D007231:Infant, Newborn; D007234:Infant, Premature; D018970:Insulin-Like Growth Factor Binding Protein 1 / Q000032:analysis / Q000378:metabolism*; D060526:Magnetic Fields; D058185:Magnetite Nanoparticles; D011237:Predictive Value of Tests; D011247:Pregnancy; D011379:Prognosis; D011446:Prospective Studies; D012372:ROC Curve; D014621:Vagina / Q000737:chemistry
chemical_list: D015415:Biomarkers; C032231:IGFBP1 protein, human; D018970:Insulin-Like Growth Factor Binding Protein 1; D058185:Magnetite Nanoparticles
keywords: Immunomagnetic reduction; Insulin-like growth factor binding protein-1; Preterm premature rupture of membranes