title: Identification of bioaccessible and uptaken phenolic compounds from strawberry fruits in in vitro digestion/Caco-2 absorption model.
abstract: Strawberry fruits are highly valued for their taste and nutritional value. However, results describing the bioaccessibility and intestinal absorption of phenolic compounds from strawberries are still scarce. In our study, a combined in vitro digestion/Caco-2 absorption model was used to mimic physiological conditions in the gastrointestinal track and identify compounds transported across intestinal epithelium. In the course of digestion, the loss of anthocyanins was noted whilst pelargonidin-3-glucoside remained the most abundant compound, amounting to nearly 12 mg per 100 g of digested strawberries. Digestion increased the amount of ellagic acid available by nearly 50%, probably due to decomposition of ellagitannins. Only trace amounts of pelargonidin-3-glucoside were found to be absorbed in the intestine model. Dihydrocoumaric acid sulphate and p-coumaric acid were identified as metabolites formed in enterocytes and released at the serosal side of the model.
authors.affiliation: Institute of Life Technologies, University of Applied Sciences and Arts Western Switzerland, Route du Rawyl 47, CH-1950 Sion, Switzerland; Division of Food Sciences, Institute of Animal Reproduction and Food Research of The Polish Academy of Sciences, Tuwima 10, 10-748 Olsztyn, Poland. Electronic address: agnieszka.kosinska@hevs.ch.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Kosińska-Cagnazzo
authors.affiliation: Institute of Life Technologies, University of Applied Sciences and Arts Western Switzerland, Route du Rawyl 47, CH-1950 Sion, Switzerland; Department of Nutrition and Food Sciences, University of Bonn, Endenicher Allee 11-13, D-53115 Bonn, Germany.
authors.forename: Sascha
authors.initials: S
authors.lastname: Diering
authors.affiliation: Institute of Life Technologies, University of Applied Sciences and Arts Western Switzerland, Route du Rawyl 47, CH-1950 Sion, Switzerland.
authors.forename: Denis
authors.initials: D
authors.lastname: Prim
authors.affiliation: Institute of Life Technologies, University of Applied Sciences and Arts Western Switzerland, Route du Rawyl 47, CH-1950 Sion, Switzerland.
authors.forename: Wilfried
authors.initials: W
authors.lastname: Andlauer
mesh_terms: D000975:Antioxidants; D018938:Caco-2 Cells / Q000737:chemistry*; D031985:Fragaria / Q000737:chemistry*; D005638:Fruit / Q000737:chemistry*; D006801:Humans; D066298:In Vitro Techniques; D010636:Phenols / Q000032:analysis*
chemical_list: D000975:Antioxidants; D010636:Phenols
keywords: Bioaccessibility; Epithelial transport; In vitro digestion; Phenolic compounds; Strawberries; p-Coumaric acid