title: Non-natural olefin cyclopropanation catalyzed by diverse cytochrome P450s and other hemoproteins.
abstract: Recent work has shown that engineered variants of cytochrome P450BM3 (CYP102A1) efficiently catalyze non-natural reactions, including carbene and nitrene transfer reactions. Given the broad substrate range of natural P450 enzymes, we set out to explore if this diversity could be leveraged to generate a broad panel of new catalysts for olefin cyclopropanation (i.e., carbene transfer). Here, we took a step towards this goal by characterizing the carbene transfer activities of four new wild-type P450s that have different native substrates. All four were active and exhibited a range of product selectivities in the model reaction: cyclopropanation of styrene by using ethyl diazoacetate (EDA). Previous work on P450BM3 demonstrated that mutation of the axial coordinating cysteine, universally conserved among P450 enzymes, to a serine residue, increased activity for this non-natural reaction. The equivalent mutation in the selected P450s was found to activate carbene transfer chemistry both in vitro and in vivo. Furthermore, serum albumins complexed with hemin were also found to be efficient in vitro cyclopropanation catalysts.
authors.affiliation: Division of Chemistry and Chemical Engineering 210-41, California Institute of Technology, 1200 E. California Boulevard, Pasadena, CA 91125 (USA).
authors.forename: Thomas
authors.initials: T
authors.lastname: Heel
authors.forename: John A
authors.initials: JA
authors.lastname: McIntosh
authors.forename: Sheel C
authors.initials: SC
authors.lastname: Dodani
authors.forename: Joseph T
authors.initials: JT
authors.lastname: Meyerowitz
authors.forename: Frances H
authors.initials: FH
authors.lastname: Arnold
mesh_terms: D000475:Alkenes / Q000737:chemistry*; D000818:Animals; D055162:Biocatalysis; D002417:Cattle; D003521:Cyclopropanes / Q000737:chemistry* / Q000378:metabolism*; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D006427:Hemin / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D008697:Methane / Q000031:analogs & derivatives / Q000737:chemistry; D009113:Muramidase / Q000378:metabolism; D012709:Serum Albumin / Q000737:chemistry / Q000378:metabolism*; D020058:Styrene / Q000737:chemistry; D013379:Substrate Specificity
chemical_list: D000475:Alkenes; D003521:Cyclopropanes; D012709:Serum Albumin; C030011:carbene; D020058:Styrene; D006427:Hemin; D003577:Cytochrome P-450 Enzyme System; D009113:Muramidase; D008697:Methane
keywords: cyclopropanation; cytochromes; enzyme catalysis; metalloenzyme