title: Use of photoactivated disinfection and platelet-rich fibrin in regenerative Endodontics.
abstract: OBJECTIVE
  Photoactivated disinfection has been used as an adjunct to conventional endodontic treatment. Its use in regenerative endodontics is not reported in literature. The aim of this case report was to describe a new proposal for pulp revascularization with disinfection of pulp canal space using a unique combination of a photosensitizer solution and low-power laser light.
  
  
  METHODS
  A 9-year-old boy came with the chief complaint of discolored upper central incisors (#8, #9). A diagnosis of pulp necrosis was made on the basis of clinical and radiographic findings. The canal was irrigated with 5.25% sodium hypochlorite solution and dried with paper points. Photodynamic therapy was used to disinfect the root canal and platelet-rich fibrin was used to revitalize the pulp. Three millimeters of gray mineral trioxide aggregate was placed directly over the platelet-rich plasma clot. Three days later, the tooth was double-sealed with permanent filling materials.
  
  
  RESULTS
  Clinical examination revealed no sensitivity to percussion or palpation tests. Radiograph revealed continued thickening of the dentinal walls, root lengthening, regression of the peri-apical lesion and apical closure. Both the roots showed complete apical closure at the 10-month follow-up. However, the teeth were not responsive to electric pulp test.
  
  
  CONCLUSIONS
  This report of pulp revascularization shows that disinfection with photodynamic therapy combined with platelet-rich fibrin leads to satisfactory root development in necrotic immature teeth.
authors.affiliation: Department of Endodontics, Government Dental College, Calicut, Kerala, India.
authors.forename: Dexton Antony
authors.initials: DA
authors.lastname: Johns
authors.affiliation: Department of Endodontics, Government Dental College, Calicut, Kerala, India.
authors.forename: Vasundara Yayathi
authors.initials: VY
authors.lastname: Shivashankar
authors.affiliation: Department of Endodontics, Government Dental College, Calicut, Kerala, India.
authors.forename: Shoba
authors.initials: S
authors.lastname: Krishnamma
authors.affiliation: Department of Prosthodontics, Kurunji Venkatramana Gowda College, Sullia, Karnataka, India.
authors.forename: Manu
authors.initials: M
authors.lastname: Johns
keywords: Photoactivated disinfection; platelet-rich fibrin; regeneration