title: Cryoprotectant-free freezing of the goat epididymal sperm.
abstract: BACKGROUND
  Cryoprotectant free approach successfully removed the impact of physical and chemical damages in preserving human sperm in a vitrification protocol. There is no any report on this technology in farm animal sperm freezing.
  
  
  OBJECTIVE
  The aim of the present study was to find the efficacy of cholesterol-loaded cyclodextrin (CLC; 1 mg per 60 million) and sucrose (0.1 and 0.2 M) on freezing of the goat epididymal sperm.
  
  
  METHODS
  Caudal epididymides (n=5 pairs) were dissected, incised and incubated in the Tris-BSA solution for 15 min, followed by swim-up at room temperature. Sperm was loaded in 0.25 mL French straws and cooled on nitrogen vapor for 3 min then immersed in liquid nitrogen and remained for 48 h. Then the straws thawed by immersing in 37 degree C waterbath for 30 sec and analyzed.
  
  
  RESULTS
  The results showed the impact of freezing on the goat epididymal sperm motility, viability and DNA fragmentation that were improved by incorporation of CLC and sucrose (0.2 M).
  
  
  CONCLUSIONS
  In conclusion, the goat epididymal sperm was frozen in a cryoprotectant-free freezing model. CLC and 0.4 M sucrose protected the goat epididymal sperm against freezing-induced damages.
authors.affiliation: Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University, Ahvaz, Iran.
authors.forename: H
authors.initials: H
authors.lastname: Katanbafzadeh
authors.affiliation: Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University, Ahvaz, Iran. fabrtir@yahoo.com.
authors.forename: F
authors.initials: F
authors.lastname: Barati
authors.affiliation: Department of Basic Sciences, Faculty of Veterinary Medicine, Shahid Chamran University, Ahvaz, Iran.
authors.forename: M
authors.initials: M
authors.lastname: Tabandeh
mesh_terms: D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D002784:Cholesterol / Q000494:pharmacology; D015925:Cryopreservation / Q000379:methods*; D003505:Cyclodextrins / Q000494:pharmacology; D053938:DNA Fragmentation / Q000187:drug effects; D004822:Epididymis / Q000166:cytology* / Q000187:drug effects / Q000502:physiology; D006041:Goats; D008297:Male; D020011:Protective Agents / Q000494:pharmacology*; D012662:Semen Preservation; D013081:Sperm Motility / Q000187:drug effects; D013094:Spermatozoa / Q000166:cytology* / Q000187:drug effects / Q000502:physiology; D013395:Sucrose / Q000494:pharmacology
chemical_list: D003505:Cyclodextrins; D020011:Protective Agents; D013395:Sucrose; D002784:Cholesterol