title: Study of NAP adsorption and assembly on the surface of HOPG.
abstract: NAP is an octapeptide that has demonstrated a neuroprotective/therapeutic efficacy at very low concentrations in preclinical studies and in a number of clinical trials. Yet little is known about its structural organization at low concentrations. Here, we have employed atomic force microscopy to investigate NAP peptide assembly on graphite in aqueous media at nanomolar concentration. High spatial resolution scans of NAP assemblies reveal their fine structure with clearly resolved single NAP units. This observation leads us to conclude that NAP molecules do not form complex self-assembled structures at nanomolar concentration when adsorbed on graphite surface.
authors.affiliation: Laboratory of Biophysics and Surface Analysis, School of Pharmacy, The University of Nottingham, Nottingham NG7 2RD, United Kingdom.
authors.forename: Vladimir V
authors.initials: VV
authors.lastname: Korolkov
authors.affiliation: Laboratory of Biophysics and Surface Analysis, School of Pharmacy, The University of Nottingham, Nottingham NG7 2RD, United Kingdom.
authors.forename: Stephanie
authors.initials: S
authors.lastname: Allen
authors.affiliation: Laboratory of Biophysics and Surface Analysis, School of Pharmacy, The University of Nottingham, Nottingham NG7 2RD, United Kingdom.
authors.forename: Clive J
authors.initials: CJ
authors.lastname: Roberts
authors.affiliation: Adams Super Center for Brain Studies, Department of Human Molecular Genetics and Biochemistry, Sackler School of Medicine and Sagol School of Neuroscience, Tel Aviv University, Tel Aviv, Israel. Electronic address: igozes@post.tau.ac.il.
authors.forename: Illana
authors.initials: I
authors.lastname: Gozes
authors.affiliation: Laboratory of Biophysics and Surface Analysis, School of Pharmacy, The University of Nottingham, Nottingham NG7 2RD, United Kingdom. Electronic address: saul.tendler@nottingham.ac.uk.
authors.forename: Saul J B
authors.initials: SJ
authors.lastname: Tendler
mesh_terms: D000327:Adsorption; D006108:Graphite / Q000737:chemistry*; D018625:Microscopy, Atomic Force; D049329:Nanostructures / Q000737:chemistry; D009842:Oligopeptides / Q000138:chemical synthesis / Q000737:chemistry*; D013499:Surface Properties; D014867:Water / Q000737:chemistry
chemical_list: D009842:Oligopeptides; D014867:Water; D006108:Graphite; C425904:davunetide
keywords: Adsorption; Atomic force microscopy; Davunetide; NAP; Peptides