title: [Etiologic characteristics of Vibrio cholerae in Guangdong province in 2009-2013].
abstract: OBJECTIVE
  To analyze the etiologic characteristics of O1/O139 Vibrio cholerae in Guangdong province in 2009-2013.
  
  
  METHODS
  Isolates from cholera cases and from the environment surveillance points were investigated by serological typing, antibiotic susceptibility testings, toxic genes detection and molecular typing to analyze the similarities and differences of the identified species.
  
  
  RESULTS
  Totally, 190 isolations of O1/O139 V. cholerae were obtained from cholera cases (16 strains) and environmental samples (174 strains) in Guangdong province in 2009-2013. The sero-types would include Inaba (3 isolates), Ogawa (7 isolates) and O139 (6 isolates) in all the isolates from the cholera cases. Ten strains from the ctxA positive cases were detected by PCR. Two Ogawa strains carried incomplete CTXΦ phage. Results from the antibiotic susceptibility test indicated that 5 strains were absolutely sensitive to 11 antibiotic discs in vitro, while another 3 strains were simultaneously resistant to 4 antibiotic discs. Except for 2 stains, all the O139 strains from the environment were ctxA negative, detected by PCR. Incomplete CTXΦ phage was found in the Inaba (53 isolates), Ogawa (22 isolates) and O139 (2 isolates), respectively. Results from the antibiotic susceptibility test exhibited that 25 strains were resistant simultaneously to 4 and/or more antibiotic discs, especially the Inaba strains from the seafoods(13 isolates). 2 Inaba strains from seafood were simultaneously resistant to 7 antibiotic discs. Results from PFGE molecular typing indicated that the PFGE types digested by Not I expressed significant diversity. Inaba and O139 strains from cases were gathered in the same clusters, while the Ogawa strains from cases scattered in different clusters but no significant correlation among these strains were found. Our results suggested that a distant genetic relationship might exist between these two different sources strains.
  
  
  CONCLUSIONS
  Complex and diverse as the virulence genes and genetic characteristics and with the grim situation of multi-drug resistant strains, all seemed important to strengthen the surveillance programs on the variation of strain types and antibiotics resistance of O1/O139 V. cholerae in Guangdong province.
authors.affiliation: Center for Disease Control and Prevention of Guangdong Province, Guangzhou 511430, China; School of Public Health and Tropic Medicine, South Medical University.
authors.forename: Baisheng
authors.initials: B
authors.lastname: Li
authors.affiliation: Center for Disease Control and Prevention of Guangdong Province, Guangzhou 511430, China.
authors.forename: Hailing
authors.initials: H
authors.lastname: Tan
authors.affiliation: National Institute of Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention.
authors.forename: Duochun
authors.initials: D
authors.lastname: Wang
authors.affiliation: Center for Disease Control and Prevention of Guangdong Province, Guangzhou 511430, China.
authors.forename: Bixia
authors.initials: B
authors.lastname: Ke
authors.affiliation: Center for Disease Control and Prevention of Guangdong Province, Guangzhou 511430, China.
authors.forename: Jingdiao
authors.initials: J
authors.lastname: Chen
authors.affiliation: Center for Disease Control and Prevention of Guangdong Province, Guangzhou 511430, China.
authors.forename: Dongmei
authors.initials: D
authors.lastname: He
authors.affiliation: Center for Disease Control and Prevention of Guangdong Province, Guangzhou 511430, China.
authors.forename: Meizhen
authors.initials: M
authors.lastname: Liu
authors.affiliation: Center for Disease Control and Prevention of Guangdong Province, Guangzhou 511430, China.
authors.forename: Changwen
authors.initials: C
authors.lastname: Ke
authors.affiliation: Center for Disease Control and Prevention of Guangdong Province, Guangzhou 511430, China; School of Public Health and Tropic Medicine, South Medical University. Email: zyh@cdcp.org.cn.
authors.forename: Yonghui
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D015373:Bacterial Typing Techniques; D002681:China / Q000453:epidemiology; D002771:Cholera / Q000453:epidemiology* / Q000382:microbiology; D024901:Drug Resistance, Multiple, Bacterial; D005798:Genes, Bacterial; D006801:Humans; D008826:Microbial Sensitivity Tests; D014734:Vibrio cholerae / Q000145:classification* / Q000187:drug effects / Q000302:isolation & purification; D014774:Virulence