title: Differential protective effects of extra virgin olive oil and corn oil in liver injury: a proteomic study.
abstract: Extra virgin olive oil (EVOO) presents benefits against chronic liver injury induced by hepatotoxins such as carbon tetrachloride (CCl4); however, the protective mechanisms remain unclear. In the present study, a two-dimensional gel based proteomic approach was constructed to explore the mechanisms. Rats are injected with CCl4 twice a week for 4 weeks to induce liver fibrosis, and were fed laboratory chow plus 20% (w/w) of either corn oil or EVOO over the entire experimental period. Histological staining, MDA assay and fibrogenesis marker gene analysis illustrate that the CCl4-treated animals fed EVOO have a lower fibrosis and lipid peroxidation level in the liver than the corn oil fed group. The proteomic study indicates that the protein expression of thioredoxin domain-containing protein 12, peroxiredoxin-1, thiosulphate sulphurtransferase, calcium-binding protein 1, Annexin A2 and heat shock cognate 71 kDa protein are higher in livers from EVOO-fed rats with the CCl4 treatment compared with those from rats fed with corn oil, whereas the expression of COQ9, cAMP-dependent protein kinase type I-alpha regulatory subunit, phenylalanine hydroxylase and glycerate kinase are lower. Our findings confirmed the benefits of EVOO against chronic liver injury, which may be attributable to the antioxidant effects, hepatocellular function regulation and hepatic metabolism modification effects of EVOO.
authors.affiliation: School of Biology and Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan, Hubei, China; Food and Nutrition Division, School of Biological Sciences, The University of Hong Kong, Hong Kong, China.
authors.forename: Hualin
authors.initials: H
authors.lastname: Wang
authors.affiliation: Food and Nutrition Division, School of Biological Sciences, The University of Hong Kong, Hong Kong, China.
authors.forename: Wat-Hung
authors.initials: WH
authors.lastname: Sit
authors.affiliation: Department of Anatomy, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, China.
authors.forename: George Lim
authors.initials: GL
authors.lastname: Tipoe
authors.affiliation: Food and Nutrition Division, School of Biological Sciences, The University of Hong Kong, Hong Kong, China. Electronic address: jmfwan@hku.hk.
authors.forename: Jennifer Man-Fan
authors.initials: JM
authors.lastname: Wan
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002251:Carbon Tetrachloride / Q000037:antagonists & inhibitors / Q000633:toxicity; D056486:Chemical and Drug Induced Liver Injury / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D003314:Corn Oil / Q000494:pharmacology*; D006801:Humans; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D008103:Liver Cirrhosis / Q000139:chemically induced / Q000378:metabolism / Q000517:prevention & control; D000069463:Olive Oil; D010938:Plant Oils / Q000494:pharmacology*; D040901:Proteomics; D051381:Rats; D011916:Rats, Inbred F344
chemical_list: D000069463:Olive Oil; D010938:Plant Oils; D003314:Corn Oil; D002251:Carbon Tetrachloride
keywords: Carbon tetrachloride; Corn oil; Extra virgin olive oil; Liver; Proteomics