title: Milk-derived ribonuclease 5 preparations induce myogenic differentiation in vitro and muscle growth in vivo.
abstract: Ribonuclease 5, also known as angiogenin, is a stable and abundant ribonuclease in milk whey protein, which is able to regulate several cellular functions, including capillary formation, neuron survival, and epithelial cell growth. Ribonuclease 5 is important for protein synthesis directly stimulating rRNA synthesis in the nucleolus. Here, we show that biologically active RNase5 can be purified from bovine milk. Furthermore, we show that milk-derived RNase5 directly stimulates muscle cell differentiation in vitro, inducing C2C12 cell differentiation and myogenesis. When supplemented into the diet of healthy adult mice, milk-derived RNase5 preparations promoted muscle weight gain and grip strength. Collectively, these data indicate that milk-derived RNase5 preparations exhibit a novel role in skeletal muscle cell function.
authors.affiliation: Biosciences Research, Department of Environment and Primary Industries, AgriBio, 5 Ring Road, Bundoora, Victoria, Australia, 3083; Agriculture Research and Development, Department of Environment and Primary Industries, 915 Mt Napier Road, Hamilton, Victoria, Australia, 3300.
authors.forename: Matthew I
authors.initials: MI
authors.lastname: Knight
authors.affiliation: Biosciences Research, Department of Environment and Primary Industries, AgriBio, 5 Ring Road, Bundoora, Victoria, Australia, 3083.
authors.forename: Angus M
authors.initials: AM
authors.lastname: Tester
authors.affiliation: Biosciences Research, Department of Environment and Primary Industries, AgriBio, 5 Ring Road, Bundoora, Victoria, Australia, 3083; Agriculture Research and Development, Department of Environment and Primary Industries, 915 Mt Napier Road, Hamilton, Victoria, Australia, 3300.
authors.forename: Matthew B
authors.initials: MB
authors.lastname: McDonagh
authors.affiliation: Murray Goulburn Co-operative Limited, 140 Dawson Street, Brunswick, Victoria, Australia, 3056.
authors.forename: Andrew
authors.initials: A
authors.lastname: Brown
authors.affiliation: Biosciences Research, Department of Environment and Primary Industries, AgriBio, 5 Ring Road, Bundoora, Victoria, Australia, 3083.
authors.forename: Jeremy
authors.initials: J
authors.lastname: Cottrell
authors.affiliation: Biosciences Research, Department of Environment and Primary Industries, AgriBio, 5 Ring Road, Bundoora, Victoria, Australia, 3083.
authors.forename: Jianghui
authors.initials: J
authors.lastname: Wang
authors.affiliation: Murray Goulburn Co-operative Limited, 140 Dawson Street, Brunswick, Victoria, Australia, 3056.
authors.forename: Peter
authors.initials: P
authors.lastname: Hobman
authors.affiliation: Biosciences Research, Department of Environment and Primary Industries, AgriBio, 5 Ring Road, Bundoora, Victoria, Australia, 3083; La Trobe University, Kingsbury Drive, Bundoora, Victoria, Australia, 3086. Electronic address: Ben.Cocks@depi.vic.gov.au.
authors.forename: Benjamin G
authors.initials: BG
authors.lastname: Cocks
mesh_terms: D000821:Animal Feed; D000818:Animals; D002417:Cattle; D002454:Cell Differentiation / Q000187:drug effects*; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects*; D019587:Dietary Supplements; D051379:Mice; D008892:Milk / Q000737:chemistry / Q000201:enzymology*; D024510:Muscle Development / Q000187:drug effects*; D018485:Muscle Fibers, Skeletal / Q000166:cytology / Q000187:drug effects; D018482:Muscle, Skeletal / Q000254:growth & development*; D012259:Ribonuclease, Pancreatic / Q000494:pharmacology*
chemical_list: C046366:angiogenin; D012259:Ribonuclease, Pancreatic
keywords: RNase5; growth factor; muscle growth; myogenic differentiation