title: Time-resolved crystallography using the Hadamard transform.
abstract: We describe a method for performing time-resolved X-ray crystallographic experiments based on the Hadamard transform, in which time resolution is defined by the underlying periodicity of the probe pulse sequence, and signal/noise is greatly improved over that for the fastest pump-probe experiments depending on a single pulse. This approach should be applicable on standard synchrotron beamlines and will enable high-resolution measurements of protein and small-molecule structural dynamics. It is also applicable to other time-resolved measurements where a probe can be encoded, such as pump-probe spectroscopy.
authors.affiliation: Astbury Centre for Structural Molecular Biology, The University of Leeds, Leeds, UK.
authors.forename: Briony A
authors.initials: BA
authors.lastname: Yorke
authors.affiliation: School of Chemistry, The University of Leeds, Leeds, UK.
authors.forename: Godfrey S
authors.initials: GS
authors.lastname: Beddard
authors.affiliation: Diamond Light Source, Harwell Science and Innovation Campus, Didcot, UK.
authors.forename: Robin L
authors.initials: RL
authors.lastname: Owen
authors.affiliation: Astbury Centre for Structural Molecular Biology, The University of Leeds, Leeds, UK.
authors.forename: Arwen R
authors.initials: AR
authors.lastname: Pearson
mesh_terms: D018360:Crystallography, X-Ray / Q000379:methods*; D010940:Plant Proteins / Q000737:chemistry*; D011487:Protein Conformation; D059629:Signal-To-Noise Ratio; D013997:Time Factors
chemical_list: D010940:Plant Proteins; C003427:thaumatin protein, plant