title: Isolation and identification of aromatic compounds in Lion's Mane Mushroom and their anticancer activities.
abstract: Lion's Mane Mushroom (Hericium erinaceum) is a traditional edible mushroom widely used in culinary applications and as an herbal medicine in East Asian countries. In the present study, two new aromatic compounds, hericerin A (1) and isohericenone J (5), along with five known compounds, isoericerin (2), hericerin (3), N-De phenylethyl isohericerin (4), hericenone J (6), and 4-[3',7'-dimethyl-2',6'-octadienyl]-2-formyl-3-hydroxy-5-methyoxybenzylalcohol (7), were isolated from a methanol extract of the fruiting bodies of H. erinaceum. The chemical structures of the compounds were determined from mass spectra and 1D- and 2D NMR spectroscopy. The anticancer effects of the isolated compounds were examined in HL-60 human acute promyelocytic leukaemia cells. Hericerin A (1) and hericerin (3) significantly reduced cell proliferation with IC50 values of 3.06 and 5.47 μM, respectively. These same compounds also induced apoptosis of HL-60 cells, accompanied by time-dependent down-regulation of p-AKT and c-myc levels. These data suggest that compounds 1 and 3 from H. erinaceum are suitable for use in potential cancer treatments.
authors.affiliation: School of Biotechnology, Yeungnam University, Gyeongsan, Gyeongbuk 712-749, Republic of Korea.
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.affiliation: College of Pharmacy, Chungnam National University, Daejeon 305-764, Republic of Korea.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhou
authors.affiliation: School of Medicine and Institute of Medical Science, Jeju National University, Jeju 690-756, Republic of Korea.
authors.forename: Eun-Ji
authors.initials: EJ
authors.lastname: Kim
authors.affiliation: School of Biotechnology, Yeungnam University, Gyeongsan, Gyeongbuk 712-749, Republic of Korea. Electronic address: shshim29@ynu.ac.kr.
authors.forename: Sang Hee
authors.initials: SH
authors.lastname: Shim
authors.affiliation: School of Medicine and Institute of Medical Science, Jeju National University, Jeju 690-756, Republic of Korea. Electronic address: pharmkhk@jejunu.ac.kr.
authors.forename: Hee Kyoung
authors.initials: HK
authors.lastname: Kang
authors.affiliation: College of Pharmacy, Chungnam National University, Daejeon 305-764, Republic of Korea. Electronic address: yhk@cnu.ac.kr.
authors.forename: Young Ho
authors.initials: YH
authors.lastname: Kim
mesh_terms: D000363:Agaricales / Q000737:chemistry*; D000818:Animals; D000970:Antineoplastic Agents / Q000627:therapeutic use*; D017209:Apoptosis; D049109:Cell Proliferation / Q000187:drug effects; D006801:Humans; D008517:Phytotherapy / Q000379:methods*
chemical_list: D000970:Antineoplastic Agents
keywords: Anticancer activity; Apoptosis; Hericerin A; Hericiaceae; Hericium erinaceum; Isohericenone J