title: Stability indicating HPLC-UV method for detection of curcumin in Curcuma longa extract and emulsion formulation.
abstract: A stability-indicating HPLC-UV method for the determination of curcumin in Curcuma longa extract and emulsion was developed. The system suitability parameters, theoretical plates (N), tailing factor (T), capacity factor (K'), height equivalent of a theoretical plate (H) and resolution (Rs) were calculated. Stress degradation studies (acid, base, oxidation, heat and UV light) of curcumin were performed in emulsion. It was found that N>6500, T<1.1, K' was 2.68-3.75, HETP about 37 and Rs was 1.8. The method was linear from 2 to 200 μg/mL with a correlation coefficient of 0.9998. The intra-day precision and accuracy for curcumin were ⩽0.87% and ⩽2.0%, while the inter-day precision and accuracy values were ⩽2.1% and ⩽-1.92. Curcumin degraded in emulsion under acid, alkali and UV light. In conclusion, the stability-indicating method could be employed to determine curcumin in bulk and emulsions.
authors.affiliation: Department of Pharmaceutical Technology, School of Pharmaceutical Sciences, Universiti Sains Malaysia, 11800 Minden, Penang, Malaysia. Electronic address: haroonkhalid80@gmail.com.
authors.forename: Haroon Khalid
authors.initials: HK
authors.lastname: Syed
authors.affiliation: Department of Pharmaceutical Technology, School of Pharmaceutical Sciences, Universiti Sains Malaysia, 11800 Minden, Penang, Malaysia. Electronic address: liewkaia@yahoo.com.
authors.forename: Kai Bin
authors.initials: KB
authors.lastname: Liew
authors.affiliation: Department of Pharmaceutical Technology, School of Pharmaceutical Sciences, Universiti Sains Malaysia, 11800 Minden, Penang, Malaysia. Electronic address: lohonnkitgabriel@yahoo.com.
authors.forename: Gabriel Onn Kit
authors.initials: GO
authors.lastname: Loh
authors.affiliation: Department of Pharmaceutical Technology, School of Pharmaceutical Sciences, Universiti Sains Malaysia, 11800 Minden, Penang, Malaysia. Electronic address: kkpeh@usm.my.
authors.forename: Kok Khiang
authors.initials: KK
authors.lastname: Peh
mesh_terms: D002626:Chemistry, Pharmaceutical; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D030024:Curcuma / Q000737:chemistry*; D003474:Curcumin / Q000032:analysis*; D004355:Drug Stability
chemical_list: D003474:Curcumin
keywords: Curcumin; Stability indicating HPLC–UV method; Stress degradation study; System suitability