title: Flow cytometric evaluation of the intracellular bacterium, Wolbachia pipientis, in mosquito cells.
abstract: Conditions for flow cytometric evaluation of the intracellular bacterium, Wolbachia pipientis, in infected mosquito cells are described. This approach will streamline investigation of Wolbachia's interactions with host cells and facilitate identification of culture conditions that select for Wolbachia-infected cells.
authors.affiliation: Department of Entomology, University of Minnesota, 1980 Folwell Ave., St. Paul, MN 55108, United States. Electronic address: fallo002@umn.edu.
authors.forename: Ann M
authors.initials: AM
authors.lastname: Fallon
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001431:Bacteriological Techniques; D002460:Cell Line; D009033:Culicidae / Q000382:microbiology; D003593:Cytoplasm / Q000382:microbiology*; D004926:Escherichia coli; D005434:Flow Cytometry / Q000379:methods; D020577:Wolbachia / Q000187:drug effects / Q000502:physiology
chemical_list: D000900:Anti-Bacterial Agents
keywords: Alphaproteobacteria; Flow cytometry; Intracellular microorganism; Mosquito cell line; Wolbachia