title: MicroRNA let-7i induced autophagy to protect T cell from apoptosis by targeting IGF1R.
abstract: MicroRNA let-7i is up-regulated in T cells from patients with Ankylosing Spondylitis (AS). In this study, we investigated the role of let-7i in T cells survival. Our results demonstrated down-regulation of insulin-like growth factor-1 receptor (IGF1R) in T cells from patients with AS. Luciferase reporter assay suggested IGF1R as direct target of let-7i. Overexpression of let-7i in Jurkat cells significantly suppressed IGF1R expression, which mimicked the action of IGF1R siRNA. IGF1R inhibition led to a strinking decrease in phosphorylation of mTOR and Akt, down-regulation of Bcl-2, up-regulation of Bax and cleavage of caspase 3 and PARP. Meanwhile, IGF1R inhibition induced autophagy. Autophagy induced by let-7i overexpression contributed to protect cells from apoptosis. Our data indicated that let-7i might control T cells fates in AS by targeting IGF1R.
authors.affiliation: Department of Rheumatology, Jining First People's Hospital, Jining, Shandong 272111, PR China.
authors.forename: Chunfeng
authors.initials: C
authors.lastname: Hou
authors.affiliation: Department of Rheumatology, Chinese Medicine Hospital in Linyi City, Linyi, Shandong 276000, PR China. Electronic address: zhumzhx@126.com.
authors.forename: Mengzhu
authors.initials: M
authors.lastname: Zhu
authors.affiliation: Clinic Institute, Jining Medical University, Jining, Shandong 272013, PR China.
authors.forename: Min
authors.initials: M
authors.lastname: Sun
authors.affiliation: Department of Center Laboratory, Shandong Provincial Hospital Affiliated to Shandong University, Jinan 250021, PR China.
authors.forename: Yanliang
authors.initials: Y
authors.lastname: Lin
mesh_terms: D017209:Apoptosis / Q000187:drug effects / Q000235:genetics*; D001343:Autophagy; D002478:Cells, Cultured; D020868:Gene Silencing; D018390:Gene Targeting / Q000379:methods*; D006801:Humans; D035683:MicroRNAs / Q000235:genetics* / Q000494:pharmacology; D017526:Receptor, IGF Type 1 / Q000235:genetics*; D013167:Spondylitis, Ankylosing / Q000235:genetics* / Q000473:pathology*; D013601:T-Lymphocytes / Q000187:drug effects / Q000473:pathology*
chemical_list: C548145:MIRNLET7 microRNA, rat; D035683:MicroRNAs; D017526:Receptor, IGF Type 1
keywords: Ankylosing Spondylitis; Apoptosis; Autophagy; IGF1R; MicroRNA let-7i