title: Improved poly(3-hydroxybutyrate) production in Escherichia coli by inactivation of cytochrome bd-II oxidase or/and NDH-II dehydrogenase in low efficient respiratory chains.
abstract: In order to redirect more carbon flux from TCA cycle into poly(3-hydroxybutyrate) (PHB) biosynthesis pathway via increasing respiratory efficiency, appB and ndh genes encoding cytochrome bd-II oxidase and NDH-II dehydrogenase were inactivated in Escherichia coli JM109/pBHR68. All appB or/and ndh knockout strains exhibited significantly increased PHB accumulation accompanying with increased NAD(P)H/NAD(P)(+) ratio and intracellular acetyl-CoA pool. Among them, the Δndh strain could accumulate up to 6.16g/L PHB from 20g/L glucose and 3.5g/L PHB from 20g/L xylose, respectively, a 1.76-fold and 3.43-fold increase compared to the wild-type control. The PHB production of this strain reached 28.23g/L in a 5-L fermentor study, which was 2.70-fold as much as that of the wild-type control. These results indicated that inactivating the cytochrome bd-II oxidase or/and NDH-II dehydrogenase of the aerobic respiratory chain is a simple and effective strategy to improve PHB biosynthesis in E. coli. To date, this is the first time to improve PHB production by inactivation of cytochrome bd-II oxidase or/and NDH-II dehydrogenase in low efficient respiratory chains.
authors.affiliation: Key Laboratory of Systems Bioengineering (Ministry of Education), Tianjin University, Tianjin 300072, People's Republic of China; SynBio Research Platform, Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, People's Republic of China; Edinburg-Tianjin Joint Research Centre for Systems Biology and Synthetic Biology, Tianjin University, Tianjin 300072, People's Republic of China.
authors.forename: Qiaojie
authors.initials: Q
authors.lastname: Liu
authors.affiliation: Key Laboratory of Systems Bioengineering (Ministry of Education), Tianjin University, Tianjin 300072, People's Republic of China; SynBio Research Platform, Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, People's Republic of China; Edinburg-Tianjin Joint Research Centre for Systems Biology and Synthetic Biology, Tianjin University, Tianjin 300072, People's Republic of China.
authors.forename: Zhenquan
authors.initials: Z
authors.lastname: Lin
authors.affiliation: Key Laboratory of Systems Bioengineering (Ministry of Education), Tianjin University, Tianjin 300072, People's Republic of China; SynBio Research Platform, Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, People's Republic of China; Edinburg-Tianjin Joint Research Centre for Systems Biology and Synthetic Biology, Tianjin University, Tianjin 300072, People's Republic of China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Systems Bioengineering (Ministry of Education), Tianjin University, Tianjin 300072, People's Republic of China; SynBio Research Platform, Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, People's Republic of China; Edinburg-Tianjin Joint Research Centre for Systems Biology and Synthetic Biology, Tianjin University, Tianjin 300072, People's Republic of China.
authors.forename: Yifan
authors.initials: Y
authors.lastname: Li
authors.affiliation: Key Laboratory of Systems Bioengineering (Ministry of Education), Tianjin University, Tianjin 300072, People's Republic of China; SynBio Research Platform, Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, People's Republic of China; Edinburg-Tianjin Joint Research Centre for Systems Biology and Synthetic Biology, Tianjin University, Tianjin 300072, People's Republic of China.
authors.forename: Zhiwen
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Key Laboratory of Systems Bioengineering (Ministry of Education), Tianjin University, Tianjin 300072, People's Republic of China; SynBio Research Platform, Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, People's Republic of China; Edinburg-Tianjin Joint Research Centre for Systems Biology and Synthetic Biology, Tianjin University, Tianjin 300072, People's Republic of China. Electronic address: chentao@tju.edu.cn.
authors.forename: Tao
authors.initials: T
authors.lastname: Chen
mesh_terms: D003573:Cytochrome b Group; D003580:Cytochromes / Q000235:genetics*; D004579:Electron Transport; D045222:Electron Transport Chain Complex Proteins / Q000235:genetics*; D004926:Escherichia coli / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000235:genetics*; D055786:Gene Knockout Techniques; D006885:Hydroxybutyrates / Q000378:metabolism*; D009245:NADH Dehydrogenase / Q000235:genetics*; D010088:Oxidoreductases / Q000235:genetics*; D011091:Polyesters / Q000378:metabolism*; D000094122:Polyhydroxybutyrates
chemical_list: D003573:Cytochrome b Group; D003580:Cytochromes; D045222:Electron Transport Chain Complex Proteins; D029968:Escherichia coli Proteins; D006885:Hydroxybutyrates; D009245:NADH Dehydrogenase; D010088:Oxidoreductases; D011091:Polyesters; C003182:poly-beta-hydroxybutyrate; D000094122:Polyhydroxybutyrates; C088908:NADH dehydrogenase II; C040077:cytochrome bd terminal oxidase complex, E coli
keywords: Acetyl-CoA; Cytochrome bd-II oxidase; Metabolic engineering; NAD(P)H; NDH-II dehydrogenase; Poly(3-hydroxybutyrate)