title: Cathepsin-B induced controlled release from peptide-capped mesoporous silica nanoparticles.
abstract: New capped silica mesoporous nanoparticles for intracellular controlled cargo release within cathepsin B expressing cells are described. Nanometric mesoporous MCM-41 supports loaded with safranin O (S1-P) or doxorubicin (S2-P) containing a molecular gate based on a cathepsin B target peptidic sequence were synthesized. Solids were designed to show "zero delivery" and to display cargo release in the presence of cathepsin B enzyme, which selectively hydrolyzed in vitro the capping peptide sequence. Controlled delivery in HeLa, MEFs WT, and MEFs lacking cathepsin B cell lines were also tested. Release of safranin O and doxorubicin in these cells took place when cathepsin B was active or present. Cells treated with S2-P showed a fall in cell viability due to nanoparticles internalization, cathepsin B hydrolysis of the capping peptide, and cytotoxic agent delivery, proving the possible use of these nanodevices as new therapeutic tools for cancer treatment.
authors.affiliation: Centro de Reconocimiento Molecular y Desarrollo Tecnológico (IDM), Unidad Mixta Universidad Politécnica de Valencia-, Universidad de Valencia (Spain); Departamento de Química, Universidad Politécnica de Valencia. Camino de Vera s/n, 46022, Valenicia (Spain); CIBER de Bioingenieria, Biomateriales y Nanomedicina (CIBER-BBN) (Spain).
authors.forename: Cristina
authors.initials: C
authors.lastname: de la Torre
authors.forename: Laura
authors.initials: L
authors.lastname: Mondragón
authors.forename: Carmen
authors.initials: C
authors.lastname: Coll
authors.forename: Félix
authors.initials: F
authors.lastname: Sancenón
authors.forename: María D
authors.initials: MD
authors.lastname: Marcos
authors.forename: Ramón
authors.initials: R
authors.lastname: Martínez-Máñez
authors.forename: Pedro
authors.initials: P
authors.lastname: Amorós
authors.forename: Enrique
authors.initials: E
authors.lastname: Pérez-Payá
authors.forename: Mar
authors.initials: M
authors.lastname: Orzáez
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology; D002401:Cathepsin B / Q000378:metabolism*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D004317:Doxorubicin / Q000737:chemistry / Q000494:pharmacology; D004337:Drug Carriers / Q000737:chemistry; D006367:HeLa Cells; D006801:Humans; D053758:Nanoparticles / Q000737:chemistry*; D010455:Peptides / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism; D016062:Porosity; D012822:Silicon Dioxide / Q000737:chemistry*
chemical_list: D000970:Antineoplastic Agents; D004337:Drug Carriers; C509968:MCM-41; D010455:Peptides; D012822:Silicon Dioxide; D004317:Doxorubicin; D002401:Cathepsin B
keywords: Cathepsin B; controlled release; gated mesoporous materials; nanoparticles; peptides