title: pH-responsive biocompatible fluorescent polymer nanoparticles based on phenylboronic acid for intracellular imaging and drug delivery.
abstract: To address current medical challenges, there is an urgent need to develop drug delivery systems with multiple functions, such as simultaneous stimuli-responsive drug release and real-time imaging. Biocompatible polymers have great potential for constructing smart multifunctional drug-delivery systems through grafting with other functional ligands. More importantly, novel biocompatible polymers with intrinsic fluorescence emission can work as theranostic nanomedicines for real-time imaging and drug delivery. Herein, we developed a highly fluorescent nanoparticle based on a phenylboronic acid-modified poly(lactic acid)-poly(ethyleneimine)(PLA-PEI) copolymer loaded with doxorubicin (Dox) for intracellular imaging and pH-responsive drug delivery. The nanoparticles exhibited superior fluorescence properties, such as fluorescence stability, no blinking and excitation-dependent fluorescence behavior. The Dox-loaded fluorescent nanoparticles showed pH-responsive drug release and were more effective in suppressing the proliferation of MCF-7 cells. In addition, the biocompatible fluorescent nanoparticles could be used as a tool for intracellular imaging and drug delivery, and the process of endosomal escape was traced by real-time imaging. These pH-responsive and biocompatible fluorescent polymer nanoparticles, based on phenylboronic acid, are promising tools for intracellular imaging and drug delivery.
authors.affiliation: Chinese Academy of Sciences Key Lab for Biological Effects of Nanomaterials and Nanosafety, National Center for Nanoscience and Technology, No. 11, First North Road, Zhongguancun, 100190 Beijing, P.R. China. liangxj@nanoctr.cn wuy@nanoctr.cn.
authors.forename: Shengliang
authors.initials: S
authors.lastname: Li
authors.forename: Kelei
authors.initials: K
authors.lastname: Hu
authors.forename: Weipeng
authors.initials: W
authors.lastname: Cao
authors.forename: Yun
authors.initials: Y
authors.lastname: Sun
authors.forename: Wang
authors.initials: W
authors.lastname: Sheng
authors.forename: Feng
authors.initials: F
authors.lastname: Li
authors.forename: Yan
authors.initials: Y
authors.lastname: Wu
authors.forename: Xing-Jie
authors.initials: XJ
authors.lastname: Liang
mesh_terms: D000970:Antineoplastic Agents / Q000008:administration & dosage / Q000493:pharmacokinetics; D001672:Biocompatible Materials / Q000138:chemical synthesis / Q000737:chemistry*; D001897:Boronic Acids / Q000138:chemical synthesis / Q000737:chemistry*; D003692:Delayed-Action Preparations; D004317:Doxorubicin / Q000008:administration & dosage / Q000493:pharmacokinetics; D016503:Drug Delivery Systems / Q000379:methods*; D004353:Drug Evaluation, Preclinical; D016650:Fluorescein-5-isothiocyanate / Q000008:administration & dosage / Q000737:chemistry / Q000493:pharmacokinetics; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007097:Imines / Q000138:chemical synthesis / Q000737:chemistry; D019344:Lactic Acid / Q000138:chemical synthesis / Q000737:chemistry; D061986:MCF-7 Cells; D057054:Molecular Imaging / Q000379:methods*; D053758:Nanoparticles / Q000737:chemistry; D011091:Polyesters; D011095:Polyethylenes / Q000138:chemical synthesis / Q000737:chemistry; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry
chemical_list: D000970:Antineoplastic Agents; D001672:Biocompatible Materials; D001897:Boronic Acids; D003692:Delayed-Action Preparations; D005456:Fluorescent Dyes; D007097:Imines; D011091:Polyesters; D011095:Polyethylenes; D011108:Polymers; C505405:poly(ethylene imine); D019344:Lactic Acid; C033616:poly(lactide); D004317:Doxorubicin; D016650:Fluorescein-5-isothiocyanate; C010686:benzeneboronic acid