title: Short communication: Nitazoxanide inhibits HIV viral replication in monocyte-derived macrophages.
abstract: We document the anti-HIV activity of nitazoxanide (NTZ), the first member of the thiazolide class of antiinfective drugs, originally effective against enteritis caused by Cryptosporidium parvum and Giardia lamblia. NTZ has been administered extensively worldwide, with no severe toxicities associated with its use. Here, we show for the first time that NTZ decreases HIV-1 replication in monocyte-derived macrophages (MDM) if present before or during HIV-1 infection. This NTZ effect is associated with downregulation of HIV-1 receptors CD4 and CCR5, and increasing gene expression of host cell anti-HIV resistance factors APOBEC3A/3G and tetherin. As NTZ is already in clinical use for other conditions, this newly described anti-HIV activity in MDM may facilitate innovative intensification strategies against HIV-1 when combined with current antiretroviral drug regimens.
authors.affiliation: 1 HIV Immunopathogenesis Laboratory, Wistar Institute , Philadelphia, Pennsylvania.
authors.forename: Bethsebah
authors.initials: B
authors.lastname: Gekonge
authors.forename: Matthew C
authors.initials: MC
authors.lastname: Bardin
authors.forename: Luis J
authors.initials: LJ
authors.lastname: Montaner
mesh_terms: D000071480:APOBEC-3G Deaminase; D019380:Anti-HIV Agents / Q000494:pharmacology*; D015703:Antigens, CD / Q000378:metabolism; D000981:Antiprotozoal Agents / Q000494:pharmacology; D015704:CD4 Antigens / Q000378:metabolism; D002478:Cells, Cultured; D003564:Cytidine Deaminase / Q000378:metabolism; D015536:Down-Regulation; D058492:Drug Repositioning; D058851:GPI-Linked Proteins / Q000378:metabolism; D015497:HIV-1 / Q000187:drug effects* / Q000502:physiology*; D006801:Humans; D008264:Macrophages / Q000821:virology*; D009574:Nitro Compounds; D011506:Proteins / Q000378:metabolism; D019713:Receptors, CCR5 / Q000378:metabolism; D013844:Thiazoles / Q000494:pharmacology*; D015854:Up-Regulation; D014779:Virus Replication / Q000187:drug effects*; D000074010:Bone Marrow Stromal Antigen 2
chemical_list: D000071480:APOBEC-3G Deaminase; D019380:Anti-HIV Agents; D015703:Antigens, CD; D000981:Antiprotozoal Agents; D015704:CD4 Antigens; D003564:Cytidine Deaminase; D058851:GPI-Linked Proteins; D009574:Nitro Compounds; D011506:Proteins; D019713:Receptors, CCR5; D013844:Thiazoles; C094572:BST2 protein, human; D000074010:Bone Marrow Stromal Antigen 2; C584933:CCR5 protein, human; C121399:APOBEC3A protein, human; C464924:APOBEC3G protein, human; C041747:nitazoxanide