title: Structure of homoserine O-acetyltransferase from Staphylococcus aureus: the first Gram-positive ortholog structure.
abstract: Homoserine O-acetyltransferase (HTA) catalyzes the formation of L-O-acetyl-homoserine from L-homoserine through the transfer of an acetyl group from acetyl-CoA. This is the first committed step required for the biosynthesis of methionine in many fungi, Gram-positive bacteria and some Gram-negative bacteria. The structure of HTA from Staphylococcus aureus (SaHTA) has been determined to a resolution of 2.45 Å. The structure belongs to the α/β-hydrolase superfamily, consisting of two distinct domains: a core α/β-domain containing the catalytic site and a lid domain assembled into a helical bundle. The active site consists of a classical catalytic triad located at the end of a deep tunnel. Structure analysis revealed some important differences for SaHTA compared with the few known structures of HTA.
authors.affiliation: Department of Chemistry and Biochemistry, The University of Toledo, Toledo, OH 43606, USA.
authors.forename: Bharani
authors.initials: B
authors.lastname: Thangavelu
authors.affiliation: Department of Chemistry and Biochemistry, The University of Toledo, Toledo, OH 43606, USA.
authors.forename: Alexander G
authors.initials: AG
authors.lastname: Pavlovsky
authors.affiliation: Department of Chemistry and Biochemistry, The University of Toledo, Toledo, OH 43606, USA.
authors.forename: Ronald
authors.initials: R
authors.lastname: Viola
mesh_terms: D000123:Acetyltransferases / Q000737:chemistry*; D000595:Amino Acid Sequence; D001051:Apoenzymes / Q000737:chemistry; D001426:Bacterial Proteins / Q000737:chemistry*; D020134:Catalytic Domain; D018360:Crystallography, X-Ray; D008958:Models, Molecular; D008969:Molecular Sequence Data; D017433:Protein Structure, Secondary; D013211:Staphylococcus aureus / Q000201:enzymology*
chemical_list: D001051:Apoenzymes; D001426:Bacterial Proteins; D000123:Acetyltransferases; C021369:homoserine O-acetyltransferase
keywords: Staphylococcus aureus; homoserine O-acetyltransferase; methionine biosynthesis; α/β-hydrolase