title: HdhCTL1 is a novel C-type lectin of abalone Haliotis discus hannai that agglutinates Gram-negative bacterial pathogens.
abstract: C-type lectins (CTLs) are Ca(2+)-dependent carbohydrate recognition proteins, which play important roles in the innate immunity of both vertebrates and invertebrates. In this study, we identified and characterized a C-type lectin (named HdhCTL1) from Pacific abalone, Haliotis discus hannai. HdhCTL1 is composed of 176 amino acid residues and shares low (23.9%) identity with the known CTL of abalone. HdhCTL1 possesses a putative signal peptide and a carbohydrate-recognition domain (CRD) typical of CTLs. The CRD of HdhCTL1 contains four disulfide bond-forming cysteine residues that are highly conserved in CTLs. HdhCTL1 mRNA was detected in a wide range of tissues and expressed abundantly in the digestive gland. Experimental infection with the bacterial pathogen Vibrio anguillarum significantly upregulated HdhCTL1 expression in a time-dependent manner. Recombinant HdhCTL1 (rHdhCTL1) purified from Escherichia coli was able to agglutinate Gram-negative bacterial pathogens. The agglutinating ability of rHdhCTL1 was abolished in the presence of mannose. These results suggest that HdhCTL1 is a novel CTL which is likely to be involved in host defense against bacterial infection.
authors.affiliation: Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China; University of Chinese Academy of Sciences, Beijing 100049, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China; China-UK-NYNU-RRes Joint Laboratory of Insect Biology, Nanyang Normal University, Nanyang, 473061 Henan, China.
authors.forename: Reng
authors.initials: R
authors.lastname: Qiu
authors.affiliation: Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China. Electronic address: huyonghua@qdio.ac.cn.
authors.forename: Yong-hua
authors.initials: YH
authors.lastname: Hu
mesh_terms: D000371:Agglutination / Q000235:genetics / Q000276:immunology*; D000704:Analysis of Variance; D000818:Animals; D001483:Base Sequence; D049872:Bivalvia / Q000235:genetics / Q000276:immunology* / Q000382:microbiology*; D003001:Cloning, Molecular; D017931:DNA Primers; D041981:Gastrointestinal Tract / Q000378:metabolism; D005786:Gene Expression Regulation / Q000276:immunology*; D005880:Gills / Q000378:metabolism; D006434:Hemocytes / Q000378:metabolism; D037181:Lectins, C-Type / Q000235:genetics* / Q000378:metabolism*; D008969:Molecular Sequence Data; D017434:Protein Structure, Tertiary / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D014733:Vibrio / Q000276:immunology
chemical_list: D017931:DNA Primers; D037181:Lectins, C-Type
keywords: Agglutination; C-type lectin; Haliotis discus hannai