title: Spectrum of Newcastle disease virus stability in gradients of temperature and pH.
abstract: Newcastle disease (ND) is one of the highly pathogenic viral diseases of avian species. The disease is endemic in many developing countries where agriculture serves as the primary source of national income. Newcastle disease virus (NDV) belongs to the family Paramyxoviridae and is well characterized member among the avian paramyxovirus serotypes. The failure of vaccination is one of the major causes of NDV outbreaks in field condition. The present study gives a brief picture about the biology of NDV genome and its proteins under different conditions of temperature and pH. Our results indicate that the NDV is non-infective above 42 °C and unstable above 72 °C. The study will be useful in defining an optimum storage condition for NDV without causing any deterioration in its viability.
authors.affiliation: Department of Biotechnology, Indian Institute of Technology Guwahati, Guwahati, Assam 781039, India.
authors.forename: Surabhi
authors.initials: S
authors.lastname: Rani
authors.affiliation: Department of Biotechnology, Indian Institute of Technology Guwahati, Guwahati, Assam 781039, India.
authors.forename: Polakshee
authors.initials: P
authors.lastname: Gogoi
authors.affiliation: Department of Biotechnology, Indian Institute of Technology Guwahati, Guwahati, Assam 781039, India. Electronic address: sachinku@iitg.ernet.in.
authors.forename: Sachin
authors.initials: S
authors.lastname: Kumar
mesh_terms: D000818:Animals; D018109:Avulavirus / Q000235:genetics; D017931:DNA Primers / Q000235:genetics; D004196:Disease Outbreaks; D016679:Genome, Viral; D006367:HeLa Cells; D006384:Hemagglutination; D006801:Humans; D006863:Hydrogen-Ion Concentration; D009522:Newcastle disease virus / Q000502:physiology*; D010252:Paramyxoviridae / Q000378:metabolism; D011200:Poultry; D012997:Solvents / Q000737:chemistry; D013696:Temperature; D014612:Vaccines / Q000737:chemistry
chemical_list: D017931:DNA Primers; D012997:Solvents; D014612:Vaccines
keywords: Temperature; Vaccine failure; Virus stability; Virus titration