title: Medicago truncatula transformation using cotyledonary explants.
abstract: Medicago truncatula has been developed into a model species for legumes. The M. truncatula genotype Jemalong A17 has been used for EST and genome sequencing. However, this genotype is difficult to regenerate from callus cultures. By using cotyledons as explants for Agrobacterium infection and direct shoot formation, this protocol allows for rapid production of transgenic plants from Jemalong A17 and other genotypes. Transgenic plants can be regenerated and established in the greenhouse in only 3-4 months after Agrobacterium-mediated transformation. Transformation frequency was in the range of 5-12 %.
authors.affiliation: Forage Improvement Division, The Samuel Roberts Noble Foundation, 2510 Sam Noble Parkway, Ardmore, OK, 73401, USA.
authors.forename: Elane
authors.initials: E
authors.lastname: Wright
authors.forename: Zeng-Yu
authors.initials: ZY
authors.lastname: Wang
mesh_terms: D016960:Agrobacterium tumefaciens; D018548:Cotyledon / Q000235:genetics; D005821:Genetic Techniques; D018525:Germination; D046913:Medicago truncatula / Q000235:genetics*; D030821:Plants, Genetically Modified / Q000235:genetics; D012639:Seeds; D013242:Sterilization / Q000379:methods; D014169:Transformation, Bacterial