title: Detection and characterization of mycoviruses in arbuscular mycorrhizal fungi by deep-sequencing.
abstract: Fungal viruses (mycoviruses) often have a significant impact not only on phenotypic expression of the host fungus but also on higher order biological interactions, e.g., conferring plant stress tolerance via an endophytic host fungus. Arbuscular mycorrhizal (AM) fungi in the phylum Glomeromycota associate with most land plants and supply mineral nutrients to the host plants. So far, little information about mycoviruses has been obtained in the fungi due to their obligate biotrophic nature. Here we provide a technical breakthrough, "two-step strategy" in combination with deep-sequencing, for virological study in AM fungi; dsRNA is first extracted and sequenced using material obtained from highly productive open pot culture, and then the presence of viruses is verified using pure material produced in the in vitro monoxenic culture. This approach enabled us to demonstrate the presence of several viruses for the first time from a glomeromycotan fungus.
authors.affiliation: Graduate School of Agriculture, Hokkaido University, Kita-ku, Kita 9, Nishi 9, Sapporo, Hokkaido, 060-8589, Japan, tatsu@res.agr.hokudai.ac.jp.
authors.forename: Tatsuhiro
authors.initials: T
authors.lastname: Ezawa
authors.forename: Yoji
authors.initials: Y
authors.lastname: Ikeda
authors.forename: Hanako
authors.initials: H
authors.lastname: Shimura
authors.forename: Chikara
authors.initials: C
authors.lastname: Masuta
mesh_terms: D059014:High-Throughput Nucleotide Sequencing / Q000379:methods*; D038821:Mycorrhizae / Q000254:growth & development / Q000821:virology*; D010802:Phylogeny; D012330:RNA, Double-Stranded / Q000302:isolation & purification; D013172:Spores, Fungal / Q000821:virology; D014713:Viruses, Unclassified / Q000235:genetics* / Q000302:isolation & purification*
chemical_list: D012330:RNA, Double-Stranded