title: Enhanced cellular content and lactate fraction of the poly(lactate-co-3-hydroxybutyrate) polyester produced in recombinant Escherichia coli by the deletion of σ factor RpoN.
abstract: A new approach at the transcriptional level was applied to lactate-based polyester production. Four σ factor disruptants, ΔrpoN, ΔrpoS, ΔfliA and ΔfecI, of Escherichia coli were used as hosts for poly(lactate-co-3-hydroxybutyrate) production from glucose. Among them, ΔrpoN caused dual positive effects of polymer production, enhanced cellular content and lactate fraction.
authors.affiliation: Division of Biotechnology and Macromolecular Chemistry, Graduate School of Engineering, Hokkaido University, N13-W8, Kita-ku, Sapporo 060-8628, Japan; CREST, JST, Sanbancho, Chiyoda-ku, Tokyo, Japan.
authors.forename: Ryosuke
authors.initials: R
authors.lastname: Kadoya
authors.affiliation: Division of Biotechnology and Macromolecular Chemistry, Graduate School of Engineering, Hokkaido University, N13-W8, Kita-ku, Sapporo 060-8628, Japan.
authors.forename: Yu
authors.initials: Y
authors.lastname: Kodama
authors.affiliation: Division of Biotechnology and Macromolecular Chemistry, Graduate School of Engineering, Hokkaido University, N13-W8, Kita-ku, Sapporo 060-8628, Japan.
authors.forename: Ken'ichiro
authors.initials: K
authors.lastname: Matsumoto
authors.affiliation: Division of Biotechnology and Macromolecular Chemistry, Graduate School of Engineering, Hokkaido University, N13-W8, Kita-ku, Sapporo 060-8628, Japan; CREST, JST, Sanbancho, Chiyoda-ku, Tokyo, Japan. Electronic address: staguchi@eng.hokudai.ac.jp.
authors.forename: Seiichi
authors.initials: S
authors.lastname: Taguchi
mesh_terms: D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000235:genetics*; D017353:Gene Deletion; D005947:Glucose / Q000378:metabolism; D019344:Lactic Acid / Q000032:analysis / Q000096:biosynthesis / Q000378:metabolism*; D011091:Polyesters / Q000032:analysis / Q000378:metabolism*; D051877:RNA Polymerase Sigma 54 / Q000172:deficiency* / Q000235:genetics*; D000094122:Polyhydroxybutyrates
chemical_list: D029968:Escherichia coli Proteins; D005947:Glucose; D019344:Lactic Acid; D011091:Polyesters; D051877:RNA Polymerase Sigma 54; C523788:poly(3-hydroxybutyrate-co-lactide); D000094122:Polyhydroxybutyrates; C497751:rpoN protein, E coli
keywords: Biobased plastic; Poly(lactic acid); Polyhydroxyalkanoate; Polyhydroxybutyrate; σ factor