title: Estrogen receptor alpha prevents bladder cancer via INPP4B inhibited akt pathway in vitro and in vivo.
abstract: Clinical reports show males have a higher bladder cancer (BCa) incidence than females. The sexual difference of BCa occurrence suggests that estrogen and its receptors may affect BCa development. Estrogen receptor alpha (ERα) is the classic receptor to convey estrogen signaling, however, the function of ERα in BCa development remains largely unknown. To understand the in vivo role of ERα in BCa development, we generated total and urothelial specific ERα knockout mice (ERαKO) and used the pre- carcinogen BBN to induce BCa. Earlier reports showed that ERα promotes breast and ovarian cancers in females. Surprisingly and of clinical importance, our results showed that ERα inhibits BCa development and loss of the ERα gene results in an earlier onset and higher incidence of BBN-induced in vivo mouse BCa. Supportively, carcinogen induced malignant transformation ability was reduced in ERα expressing urothelial cells as compared to ERα negative cells. Mechanism studies suggest that ERα could control the expression of INPP4B to reduce AKT activity and consequently reduce BCa cell growth. In addition, IHC staining of clinical sample analyses show that INPP4B expression, in correlation with reduced ERα, is significantly reduced in human BCa specimens. Together, this is the first report using the in vivo cre-loxP gene knockout mouse model to characterize ERα roles in BCa development. Our studies provide multiple in vitro cell studies and in vivo animal model data as well as human BCa tissue analyses to prove ERα plays a protective role in BCa initiation and growth at least partly via modulating the INPP4B/Akt pathway.
authors.affiliation: Departments of Urology and Pathology, University of Rochester Medical Center, Rochester, NY 14642. Contributed equally.
authors.forename: Iawen
authors.initials: I
authors.lastname: Hsu
authors.affiliation: Departments of Urology and Pathology, University of Rochester Medical Center, Rochester, NY 14642. Contributed equally.
authors.forename: Chiuan-Ren
authors.initials: CR
authors.lastname: Yeh
authors.affiliation: Departments of Urology and Pathology, University of Rochester Medical Center, Rochester, NY 14642.
authors.forename: Spencer
authors.initials: S
authors.lastname: Slavin
authors.affiliation: Departments of Pathology, Urology, and Oncology, The Johns Hopkins Hospital, Baltimore, MD 21231.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Miyamoto
authors.affiliation: Departments of Pathology, Urology, and Oncology, The Johns Hopkins Hospital, Baltimore, MD 21231.
authors.forename: George J
authors.initials: GJ
authors.lastname: Netto
authors.affiliation: Departments of Urology and Pathology, University of Rochester Medical Center, Rochester, NY 14642.
authors.forename: Yu-Chieh
authors.initials: YC
authors.lastname: Tsai
authors.affiliation: Department of Biochemistry and Biophysics, University of Rochester Medical Center, Rochester, NY 14642.
authors.forename: Mesut
authors.initials: M
authors.lastname: Muyan
authors.affiliation: Department of Urology, New York University, School of Medicine, NY 10016.
authors.forename: Xue-Ru
authors.initials: XR
authors.lastname: Wu
authors.affiliation: Departments of Urology and Pathology, University of Rochester Medical Center, Rochester, NY 14642.
authors.forename: Edward M
authors.initials: EM
authors.lastname: Messing
authors.affiliation: Departments of Hematology and Oncology, University of Rochester Medical Center, Rochester, NY 14642.
authors.forename: Elizabeth A
authors.initials: EA
authors.lastname: Guancial
authors.affiliation: Departments of Urology and Pathology, University of Rochester Medical Center, Rochester, NY 14642.
authors.forename: Shuyuan
authors.initials: S
authors.lastname: Yeh
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D002295:Carcinoma, Transitional Cell / Q000235:genetics* / Q000378:metabolism; D004195:Disease Models, Animal; D047628:Estrogen Receptor alpha / Q000235:genetics*; D005260:Female; D006801:Humans; D015151:Immunoblotting; D007150:Immunohistochemistry; D066298:In Vitro Techniques; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008875:Middle Aged; D010744:Phosphoric Monoester Hydrolases / Q000378:metabolism*; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism*; D060888:Real-Time Polymerase Chain Reaction; D015398:Signal Transduction / Q000502:physiology; D046888:Tissue Array Analysis; D001749:Urinary Bladder Neoplasms / Q000235:genetics* / Q000378:metabolism
chemical_list: D047628:Estrogen Receptor alpha; D051057:Proto-Oncogene Proteins c-akt; D010744:Phosphoric Monoester Hydrolases; C053153:phosphatidylinositol-3,4-bisphosphate 4-phosphatase