title: Soybean [Glycine max (L.) Merr].
abstract: In this chapter we describe an Agrobacterium tumefaciens transformation method of soybean that utilizes mature half seeds and regeneration from the cotyledonary node region. This method results in fertile transformed soybean plants and transgenic seed in approximately 9 months. Using mature half seeds as starting material has proven to be a reliable method that does not require additional wounding for infection to occur. We have continued to make improvements in the protocol, resulting in an efficient plant regeneration system.
authors.affiliation: Center for Plant Transformation, Plant Sciences Institute, and Department of Agronomy, Iowa State University, Ames, IA, 50011-1010, USA, dluth@iastate.edu.
authors.forename: Diane
authors.initials: D
authors.lastname: Luth
authors.forename: Katey
authors.initials: K
authors.lastname: Warnberg
authors.forename: Kan
authors.initials: K
authors.lastname: Wang
mesh_terms: D000383:Agriculture / Q000379:methods; D016960:Agrobacterium tumefaciens / Q000235:genetics; D005821:Genetic Techniques; D018517:Plant Roots / Q000235:genetics / Q000254:growth & development; D018520:Plant Shoots / Q000235:genetics / Q000254:growth & development; D030821:Plants, Genetically Modified; D012639:Seeds / Q000235:genetics / Q000254:growth & development; D012641:Selection, Genetic; D013025:Glycine max / Q000235:genetics* / Q000254:growth & development; D013242:Sterilization; D014169:Transformation, Bacterial