title: Study on the main components interaction from Flos Lonicerae and Fructus Forsythiae and their dissolution in vitro and intestinal absorption in rats.
abstract: The Flos Lonicerae-Fructus Forsythiae herb couple is the basic components of Chinese herbal preparations (Shuang-Huang-Lian tablet, Yin-Qiao-Jie-Du tablet and Fufang Qin-Lan oral liquid), and its pharmacological effects were significantly higher than that in Flos Lonicerae or Fructus Forsythiae, but the reasons remained unknown. In the present study, pattern recognition analysis (hierarchical cluster analysis (HCA) and principal component analysis (PCA)) combined with UHPLC-ESI/LTQ-Orbitrap MS system were performed to study the chemical constitution difference between co-decoction and mixed decoction in the term of chemistry. Besides, the pharmacokinetics in vivo and intestinal absorption in vitro combined with pattern recognition analysis were used to reveal the discrepancy between herb couple and single herbs in the view of biology. The observation from the chemical view in vitro showed that there was significant difference in quantity between co-decoction and mixed decoction by HCA, and the exposure level of isoforsythoside and 3, 5-dicaffeoylquinic acid in co-decoction, higher than that in mixed decoction, directly resulted in the discrepancy between co-decoction and mixed decoction using both PCA and HCA. The observation from the pharmacokinetics displayed that the exposure level in vivo of neochlorogenic acid, 3, 4-dicaffeoylquinic acid, isoforsythoside and forsythoside A, higher than that in single herbs, was the main factor contributing to the difference by both PCA and HCA, interestingly consistent with the results obtained from Caco-2 cells in vitro, which indicated that it was because of intestinal absorption improvement of neochlorogenic acid, 3, 4-dicaffeoylquinic acid, isoforsythoside and forsythoside A that resulted in a better efficacy of herb couple than that of single herbs from the perspective of biology. The results above illustrated that caffeic acid derivatives in Flos Lonicerae-Fructus Forsythiae herb couple could be considered as chemical markers for quality control of its preparations.
authors.affiliation: College of Pharmacy, Nan'jing University of Chinese Medicine, Nan'jing, People's Republic of China; Jiang'su Engineering Research Center for Efficient Delivery System of TCM, Nan'jing, People's Republic of China; Nan'jing Engineering Research Center for Industrialization of Chinese Medicine Pellets, Nan'jing, People's Republic of China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhou
authors.affiliation: Laboratory of New Drug Delivery System of Chinese Meteria Medica, Jiang'su Provinical Academy of Chinese Medicine, Nan'jing, People's Republic of China.
authors.forename: Xiaobin
authors.initials: X
authors.lastname: Tan
authors.affiliation: Jiangsu Key Laboratory of Pediatric Respiratory Disease, Institute of Paediatrics, Nan'jing University of Chinese Medicine, Nan'jing, People's Republic of China.
authors.forename: Jinjun
authors.initials: J
authors.lastname: Shan
authors.affiliation: Jiangsu Key Laboratory of Pediatric Respiratory Disease, Institute of Paediatrics, Nan'jing University of Chinese Medicine, Nan'jing, People's Republic of China.
authors.forename: Shouchuan
authors.initials: S
authors.lastname: Wang
authors.affiliation: College of Pharmacy, Nan'jing University of Chinese Medicine, Nan'jing, People's Republic of China.
authors.forename: Ailing
authors.initials: A
authors.lastname: Yin
authors.affiliation: College of Pharmacy, Nan'jing University of Chinese Medicine, Nan'jing, People's Republic of China.
authors.forename: Baochang
authors.initials: B
authors.lastname: Cai
authors.affiliation: College of Pharmacy, Nan'jing University of Chinese Medicine, Nan'jing, People's Republic of China; Jiang'su Engineering Research Center for Efficient Delivery System of TCM, Nan'jing, People's Republic of China; Nan'jing Engineering Research Center for Industrialization of Chinese Medicine Pellets, Nan'jing, People's Republic of China.
authors.forename: Liuqing
authors.initials: L
authors.lastname: Di
mesh_terms: D000818:Animals; D018938:Caco-2 Cells; D016000:Cluster Analysis; D004365:Drugs, Chinese Herbal / Q000032:analysis* / Q000493:pharmacokinetics*; D031657:Forsythia / Q000737:chemistry*; D006801:Humans; D007408:Intestinal Absorption; D031239:Lonicera / Q000737:chemistry*; D008297:Male; D025341:Principal Component Analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D012995:Solubility
chemical_list: D004365:Drugs, Chinese Herbal; C516400:shuang-huang-lian