title: Cell physiology of the biotechnological relevant bacterium Bacillus pumilus-an omics-based approach.
abstract: Members of the species Bacillus pumilus get more and more in focus of the biotechnological industry as potential new production strains. Based on exoproteome analysis, B. pumilus strain Jo2, possessing a high secretion capability, was chosen for an omics-based investigation. The proteome and metabolome of B. pumilus cells growing either in minimal or complex medium was analyzed. In total, 1542 proteins were identified in growing B. pumilus cells, among them 1182 cytosolic proteins, 297 membrane and lipoproteins and 63 secreted proteins. This accounts for about 43% of the 3616 proteins encoded in the B. pumilus Jo2 genome sequence. By using GC-MS, IP-LC/MS and H NMR methods numerous metabolites were analyzed and assigned to reconstructed metabolic pathways. In the genome sequence a functional secretion system including the components of the Sec- and Tat-secretion machinery was found. Analysis of the exoproteome revealed secretion of about 70 proteins with predicted secretion signals. In addition, selected production-relevant genome features such as restriction modification systems and NRPS clusters of B. pumilus Jo2 are discussed.
authors.affiliation: Institute for Microbiology, Ernst-Moritz-Arndt University, Greifswald, Germany. Electronic address: handtkes@uni-greifswald.de.
authors.forename: Stefan
authors.initials: S
authors.lastname: Handtke
authors.affiliation: Department of Genomic and Applied Microbiology & Göttingen Genomics Laboratory, Institute of Microbiology and Genetics, Georg-August-University Göttingen, Göttingen, Germany. Electronic address: svollan@gwdg.de.
authors.forename: Sonja
authors.initials: S
authors.lastname: Volland
authors.affiliation: Institute of Biochemistry, Ernst-Moritz-Arndt University, Greifswald, Germany. Electronic address: methling@uni-greifswald.de.
authors.forename: Karen
authors.initials: K
authors.lastname: Methling
authors.affiliation: Institute for Microbiology, Ernst-Moritz-Arndt University, Greifswald, Germany. Electronic address: dirkalbr@uni-greifswald.de.
authors.forename: Dirk
authors.initials: D
authors.lastname: Albrecht
authors.affiliation: Institute for Microbiology, Ernst-Moritz-Arndt University, Greifswald, Germany. Electronic address: dbecher@uni-greifswald.de.
authors.forename: Dörte
authors.initials: D
authors.lastname: Becher
authors.affiliation: Institute of Biochemistry, Ernst-Moritz-Arndt University, Greifswald, Germany. Electronic address: jenny-nehls@gmx.de.
authors.forename: Jenny
authors.initials: J
authors.lastname: Nehls
authors.affiliation: Department of Chemistry and Biotechnology, Aachen University of Applied Sciences, Heinrich-Mußmannstr. 1, 52428 Jülich, Germany. Electronic address: bongaerts@fh-aachen.de.
authors.forename: Johannes
authors.initials: J
authors.lastname: Bongaerts
authors.affiliation: AB Enzymes GmbH, Darmstadt, Germany. Electronic address: karl-heinz.maurer@abenzymes.com.
authors.forename: Karl-Heinz
authors.initials: KH
authors.lastname: Maurer
authors.affiliation: Institute of Biochemistry, Ernst-Moritz-Arndt University, Greifswald, Germany. Electronic address: lalk@uni.greifswald.de.
authors.forename: Michael
authors.initials: M
authors.lastname: Lalk
authors.affiliation: Department of Genomic and Applied Microbiology & Göttingen Genomics Laboratory, Institute of Microbiology and Genetics, Georg-August-University Göttingen, Göttingen, Germany. Electronic address: hlieseg@gwdg.de.
authors.forename: Heiko
authors.initials: H
authors.lastname: Liesegang
authors.affiliation: Institute for Microbiology, Ernst-Moritz-Arndt University, Greifswald, Germany. Electronic address: birgit.voigt@uni-greifswald.de.
authors.forename: Birgit
authors.initials: B
authors.lastname: Voigt
authors.affiliation: Department of Genomic and Applied Microbiology & Göttingen Genomics Laboratory, Institute of Microbiology and Genetics, Georg-August-University Göttingen, Göttingen, Germany. Electronic address: rdaniel@gwdg.de.
authors.forename: Rolf
authors.initials: R
authors.lastname: Daniel
authors.affiliation: Institute for Microbiology, Ernst-Moritz-Arndt University, Greifswald, Germany. Electronic address: hecker@uni-greifswald.de.
authors.forename: Michael
authors.initials: M
authors.lastname: Hecker
mesh_terms: D001407:Bacillus / Q000235:genetics / Q000378:metabolism*; D001426:Bacterial Proteins / Q000378:metabolism*; D016680:Genome, Bacterial; D055442:Metabolome; D020543:Proteome
chemical_list: D001426:Bacterial Proteins; D020543:Proteome
keywords: B. pumilus; Cytosolic proteome; Exoproteome; Jo2 genome sequence; Metabolome