title: Heterobifunctional PEG ligands for bioconjugation reactions on iron oxide nanoparticles.
abstract: Ever since iron oxide nanoparticles have been recognized as promising scaffolds for biomedical applications, their surface functionalization has become even more important. We report the synthesis of a novel polyethylene glycol-based ligand that combines multiple advantageous properties for these applications. The ligand is covalently bound to the surface via a siloxane group, while its polyethylene glycol backbone significantly improves the colloidal stability of the particle in complex environments. End-capping the molecule with a carboxylic acid introduces a variety of coupling chemistry possibilities. In this study an antibody targeting plasminogen activator inhibitor-1 was coupled to the surface and its presence and binding activity was assessed by enzyme-linked immunosorbent assay and surface plasmon resonance experiments. The results indicate that the ligand has high potential towards biomedical applications where colloidal stability and advanced functionality is crucial.
authors.affiliation: Department of Chemistry, KU Leuven, Heverlee, Leuven, Belgium.
authors.forename: Maarten
authors.initials: M
authors.lastname: Bloemen
authors.affiliation: Department of Pharmaceutical and Pharmacological Sciences, KU Leuven, O&N II, Leuven, Belgium.
authors.forename: Thomas
authors.initials: T
authors.lastname: Van Stappen
authors.affiliation: Department of Chemistry, KU Leuven, Heverlee, Leuven, Belgium.
authors.forename: Pieter
authors.initials: P
authors.lastname: Willot
authors.affiliation: BIOSYST-MeBioS, KU Leuven, Heverlee, Leuven, Belgium.
authors.forename: Jeroen
authors.initials: J
authors.lastname: Lammertyn
authors.affiliation: Department of Chemistry, KU Leuven, Heverlee, Leuven, Belgium.
authors.forename: Guy
authors.initials: G
authors.lastname: Koeckelberghs
authors.affiliation: PharmAbs, The KU Leuven Antibody Center, KU Leuven, O&N II, Leuven, Belgium.
authors.forename: Nick
authors.initials: N
authors.lastname: Geukens
authors.affiliation: Department of Pharmaceutical and Pharmacological Sciences, KU Leuven, O&N II, Leuven, Belgium.
authors.forename: Ann
authors.initials: A
authors.lastname: Gils
authors.affiliation: Department of Chemistry, KU Leuven, Heverlee, Leuven, Belgium.
authors.forename: Thierry
authors.initials: T
authors.lastname: Verbiest
mesh_terms: D055910:Antibodies, Immobilized / Q000737:chemistry*; D057930:Click Chemistry; D004797:Enzyme-Linked Immunosorbent Assay; D005290:Ferric Compounds / Q000737:chemistry*; D006801:Humans; D008024:Ligands; D053758:Nanoparticles / Q000737:chemistry*; D017395:Plasminogen Activator Inhibitor 1 / Q000032:analysis; D011092:Polyethylene Glycols / Q000737:chemistry*; D020349:Surface Plasmon Resonance
chemical_list: D055910:Antibodies, Immobilized; D005290:Ferric Compounds; D008024:Ligands; D017395:Plasminogen Activator Inhibitor 1; C000499:ferric oxide; D011092:Polyethylene Glycols