title: Visualizing the replication of respiratory syncytial virus in cells and in living mice.
abstract: Respiratory syncytial virus (RSV) is the most important cause of severe lower-respiratory tract disease in calves and young children, yet no human vaccine nor efficient curative treatments are available. Here we describe a recombinant human RSV reverse genetics system in which the red fluorescent protein (mCherry) or the firefly luciferase (Luc) genes are inserted into the RSV genome. Expression of mCherry and Luc are correlated with infection rate, allowing the monitoring of RSV multiplication in cell culture. Replication of the Luc-encoding virus in living mice can be visualized by bioluminescent imaging, bioluminescence being detected in the snout and lungs of infected mice after nasal inoculation. We propose that these recombinant viruses are convenient and valuable tools for screening of compounds active against RSV, and can be used as an extremely sensitive readout for studying effects of antiviral therapeutics in living mice.
authors.affiliation: 1] Unité de Virologie et Immunologie Moleculaires (UR892), INRA, Jouy-en-Josas F78352, France [2] Physiopathologie et diagnostic des infections microbiennes, EA3647-EPIM, UFR des Sciences de la Santé Simone Veil-UVSQ, 2 avenue de la Source de la Bièvre, 78180 Montigny-Le-Bretonneux, France [3] AP-HP, Hôpital Ambroise Paré, Laboratoire de Microbiologie, Boulogne-Billancourt 92100, France.
authors.forename: Marie-Anne
authors.initials: MA
authors.lastname: Rameix-Welti
authors.affiliation: Unité de Virologie et Immunologie Moleculaires (UR892), INRA, Jouy-en-Josas F78352, France.
authors.forename: Ronan
authors.initials: R
authors.lastname: Le Goffic
authors.affiliation: Unité de Virologie et Immunologie Moleculaires (UR892), INRA, Jouy-en-Josas F78352, France.
authors.forename: Pierre-Louis
authors.initials: PL
authors.lastname: Hervé
authors.affiliation: 1] Unité de Virologie et Immunologie Moleculaires (UR892), INRA, Jouy-en-Josas F78352, France [2] Physiopathologie et diagnostic des infections microbiennes, EA3647-EPIM, UFR des Sciences de la Santé Simone Veil-UVSQ, 2 avenue de la Source de la Bièvre, 78180 Montigny-Le-Bretonneux, France.
authors.forename: Julien
authors.initials: J
authors.lastname: Sourimant
authors.affiliation: Unité de Virologie et Immunologie Moleculaires (UR892), INRA, Jouy-en-Josas F78352, France.
authors.forename: Aude
authors.initials: A
authors.lastname: Rémot
authors.affiliation: Unité de Virologie et Immunologie Moleculaires (UR892), INRA, Jouy-en-Josas F78352, France.
authors.forename: Sabine
authors.initials: S
authors.lastname: Riffault
authors.affiliation: Infection Innovative Medicines Unit, AstraZeneca R&D Boston, 35 Gatehouse Drive, Waltham, Massachusetts 02451, USA.
authors.forename: Qin
authors.initials: Q
authors.lastname: Yu
authors.affiliation: Unité de Virologie et Immunologie Moleculaires (UR892), INRA, Jouy-en-Josas F78352, France.
authors.forename: Marie
authors.initials: M
authors.lastname: Galloux
authors.affiliation: 1] Physiopathologie et diagnostic des infections microbiennes, EA3647-EPIM, UFR des Sciences de la Santé Simone Veil-UVSQ, 2 avenue de la Source de la Bièvre, 78180 Montigny-Le-Bretonneux, France [2] AP-HP, Hôpital Ambroise Paré, Laboratoire de Microbiologie, Boulogne-Billancourt 92100, France.
authors.forename: Elyanne
authors.initials: E
authors.lastname: Gault
authors.affiliation: Unité de Virologie et Immunologie Moleculaires (UR892), INRA, Jouy-en-Josas F78352, France.
authors.forename: Jean-François
authors.initials: JF
authors.lastname: Eléouët
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D000998:Antiviral Agents / Q000737:chemistry; D001483:Base Sequence; D004261:DNA Replication; D018076:DNA, Complementary / Q000378:metabolism; D005260:Female; D005453:Fluorescence; D005822:Genetic Vectors; D006801:Humans; D008156:Luciferases / Q000378:metabolism; D049449:Luminescence; D008164:Luminescent Proteins / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D008856:Microscopy, Fluorescence; D008957:Models, Genetic; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000378:metabolism; D018113:Respiratory Syncytial Virus, Human; D012136:Respiratory Syncytial Viruses / Q000472:pathogenicity / Q000502:physiology*; D014760:Viral Fusion Proteins / Q000235:genetics; D014779:Virus Replication; D000097573:Red Fluorescent Protein
chemical_list: D000914:Antibodies, Viral; D000998:Antiviral Agents; D018076:DNA, Complementary; D008156:Luciferases; D008164:Luminescent Proteins; D011994:Recombinant Proteins; D014760:Viral Fusion Proteins; D000097573:Red Fluorescent Protein