title: [Sequencing and analysis of N gene of street rabies virus isolated from different hosts in Zhejiang province].
abstract: OBJECTIVE
  To elucidate the characteristics of genetic variability and its relationship with prevalence, through sequencing and analysis of N gene among street rabies virus isolated from different hosts (homo sapiens, ferret badger, dog) in Zhejiang province.
  
  
  METHODS
  Samples were screened and confirmed by direct fluorescence assay and reverse transcript PCR. Sequences were analyzed using bio-information software.
  
  
  RESULTS
  Eighteen street rabies virus strains were identified, including 2 from homo sapiens, 5 from ferret badger, and 11 from dog. Similarities of N gene and N protein were calculated to be 89.7%-100.0% and 98.4%-100.0% respectively. Mutations occurred in N gene were almost non-sense mutations. In addition,Data from phylogenetic analysis showed that all these strains could be classified into traditional genotype 1.
  
  
  CONCLUSIONS
  The prevalence of rabies viruses among different hosts in Zhejiang province had certain regional properties. Rabies viruses isolated from the same kind of host or from the same/adjacent county/counties had the closest relationship. However, the characteristics of rabies virus prevalent in homo sapiens were somewhat complicated. In summary, the transmission of street rabies virus in Zhejiang province was from dogs to ferret badgers and homo sapiens, and the virus could circulate and cross-regional transmit among dogs and ferret badgers.
authors.affiliation: Lishui Center for Disease Control and Prevention, Zhejiang 323000, China.
authors.forename: Xiaoguang
authors.initials: X
authors.lastname: Wang
authors.affiliation: Lishui Center for Disease Control and Prevention, Zhejiang 323000, China.
authors.forename: Yongliang
authors.initials: Y
authors.lastname: Lei
authors.affiliation: Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Tao
authors.affiliation: Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention.
authors.forename: Hao
authors.initials: H
authors.lastname: Li
authors.affiliation: Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention.
authors.forename: Xinxin
authors.initials: X
authors.lastname: Shen
authors.affiliation: Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention.
authors.forename: Pengcheng
authors.initials: P
authors.lastname: Yu
authors.affiliation: Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention.
authors.forename: Cuiping
authors.initials: C
authors.lastname: Yin
authors.affiliation: Department of Genetic Engineering, Wuhan Institute of Biologic Products.
authors.forename: Shengli
authors.initials: S
authors.lastname: Meng
authors.affiliation: Zhejiang Provincial Center for Disease Control and Prevention.
authors.forename: Xinying
authors.initials: X
authors.lastname: Wang
authors.affiliation: Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention. Email: qtang04@sina.com.
authors.forename: Qing
authors.initials: Q
authors.lastname: Tang
mesh_terms: D000818:Animals; D002681:China / Q000453:epidemiology; D004252:DNA Mutational Analysis; D004285:Dogs / Q000821:virology; D006801:Humans; D045969:Mustelidae / Q000821:virology; D012367:RNA, Viral / Q000235:genetics; D011818:Rabies / Q000453:epidemiology; D011820:Rabies virus / Q000235:genetics* / Q000302:isolation & purification; D014759:Viral Envelope Proteins / Q000235:genetics*
chemical_list: D012367:RNA, Viral; D014759:Viral Envelope Proteins; C097733:glycoprotein N, Pseudorabies virus