title: Genetic Diversity of Pectobacterium carotovorum subsp. brasiliensis Isolated in Korea.
abstract: The plant pathogenic bacterial genus Pectobacteirum consists of heterogeneous strains. The P. carotovorum species is a complex strain showing divergent characteristics, and a new subspecies named P. carotovorum subsp. brasiliensis has been identified recently. In this paper, we re-identified the P. carotovorum subsp. brasiliensis isolates from those classified under the subspecies carotovorum and newly isolated P. carotovorum subsp. brasiliensis strains. All isolates were able to produce plant cell-wall degrading enzymes such as pectate lyase, polygalacturonase, cellulase and protease. We used genetic and biochemical methods to examine the diversity of P. carotovorum subsp. brasiliensis isolates, and found genetic diversity within the brasiliensis subsp. isolates in Korea. The restriction fragment length polymorphism analysis based on the recA gene revealed a unique pattern for the brasiliensis subspecies. The Korean brasiliensis subsp. isolates were divided into four clades based on pulsed-field gel electrophoresis. However, correlations between clades and isolated hosts or year could not be found, suggesting that diverse brasiliensis subsp. isolates existed.
authors.affiliation: Division of Microbial Safety, National Academy of Agricultural Science, Rural Development Administration, Suwon 441-707, Korea.
authors.forename: Dong Hwan
authors.initials: DH
authors.lastname: Lee
authors.affiliation: Division of Microbial Safety, National Academy of Agricultural Science, Rural Development Administration, Suwon 441-707, Korea.
authors.forename: Jin-Beom
authors.initials: JB
authors.lastname: Kim
authors.affiliation: Division of Microbial Safety, National Academy of Agricultural Science, Rural Development Administration, Suwon 441-707, Korea.
authors.forename: Jeong-A
authors.initials: JA
authors.lastname: Lim
authors.affiliation: Department of Integrative Plant Science, Chung-Ang University, Anseong 456-756, Korea.
authors.forename: Sang-Wook
authors.initials: SW
authors.lastname: Han
authors.affiliation: Division of Microbial Safety, National Academy of Agricultural Science, Rural Development Administration, Suwon 441-707, Korea.
authors.forename: Sunggi
authors.initials: S
authors.lastname: Heu
keywords: 16S rDNA; PFGE; Pectobacterium catorovorum subsp. brasiliensis; RFLP; recA