title: Cross-linked polyethylene glycol beads to separate 99mTc-pertechnetate from low-specific-activity molybdenum.
abstract: We report a kit-based approach for the purification of sodium pertechnetate ((99m)TcO4 (-)) from solutions with high MoO4 (2-) content.
  
  
  METHODS
  Cross-linked polyethylene glycol resins (ChemMatrix) were used to separate (99m)Tc and molybdenum in 4N NaOH. The resins were loaded at various flow rates and eluted with water to release (99m)Tc. The (99m)Tc solution was passed through a cation exchange resin and an alumina cartridge, followed by saline elution. This process was tested with cyclotron-produced (99m)Tc using an automated system and disposable kits.
  
  
  RESULTS
  Optimal results were obtained by loading 500 mg of resin at flow rates of up to 3.1 mL/min, with quantitative extraction of (99m)Tc from the molybdate solution and complete release of (99m)Tc after elution with water. The automated system was highly efficient at isolating Na(99m)TcO4 within minutes, with a recovery rate of 92.7% ± 1.1% (mean ± SD) using cyclotron-produced (99m)Tc.
  
  
  CONCLUSIONS
  ChemMatrix resins were highly effective at separating (99m)TcO4 (-) from molybdate solutions.
authors.affiliation: BC Cancer Agency, Vancouver, British Columbia, Canada Department of Radiology, University of British Columbia, Vancouver, British Columbia, Canada fbenard@bccrc.ca.
authors.forename: François
authors.initials: F
authors.lastname: Bénard
authors.affiliation: TRIUMF, Vancouver, British Columbia, Canada; and.
authors.forename: Stefan K
authors.initials: SK
authors.lastname: Zeisler
authors.affiliation: BC Cancer Agency, Vancouver, British Columbia, Canada.
authors.forename: Milan
authors.initials: M
authors.lastname: Vuckovic
authors.affiliation: BC Cancer Agency, Vancouver, British Columbia, Canada Department of Radiology, University of British Columbia, Vancouver, British Columbia, Canada.
authors.forename: Kuo-Shyan
authors.initials: KS
authors.lastname: Lin
authors.affiliation: BC Cancer Agency, Vancouver, British Columbia, Canada.
authors.forename: Zhengxing
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: BC Cancer Agency, Vancouver, British Columbia, Canada.
authors.forename: Nadine
authors.initials: N
authors.lastname: Colpo
authors.affiliation: Department of Physics and Astronomy, University of British Columbia, Vancouver, British Columbia, Canada.
authors.forename: Xinchi
authors.initials: X
authors.lastname: Hou
authors.affiliation: BC Cancer Agency, Vancouver, British Columbia, Canada TRIUMF, Vancouver, British Columbia, Canada; and.
authors.forename: Thomas J
authors.initials: TJ
authors.lastname: Ruth
authors.affiliation: Department of Radiology, University of British Columbia, Vancouver, British Columbia, Canada TRIUMF, Vancouver, British Columbia, Canada; and.
authors.forename: Paul
authors.initials: P
authors.lastname: Schaffer
mesh_terms: D000535:Aluminum / Q000737:chemistry; D001331:Automation; D002852:Chromatography, Ion Exchange; D003432:Cross-Linking Reagents / Q000737:chemistry; D017357:Cyclotrons; D007475:Ion Exchange Resins; D008982:Molybdenum / Q000737:chemistry* / Q000302:isolation & purification*; D011092:Polyethylene Glycols / Q000737:chemistry*; D011786:Quality Control; D011868:Radioisotopes; D015203:Reproducibility of Results; D012972:Sodium Hydroxide / Q000737:chemistry; D013670:Sodium Pertechnetate Tc 99m / Q000737:chemistry* / Q000302:isolation & purification*
chemical_list: D003432:Cross-Linking Reagents; D007475:Ion Exchange Resins; D011868:Radioisotopes; C044659:molybdate; D011092:Polyethylene Glycols; D012972:Sodium Hydroxide; D008982:Molybdenum; D013670:Sodium Pertechnetate Tc 99m; D000535:Aluminum
keywords: 99mTc-pertechnetate; generators; molybdenum