title: MitoBlue: a nontoxic and photostable blue-emitting dye that selectively labels functional mitochondria.
abstract: We report the discovery of a fluorogenic dye, N(1),N(3)-di(2-aminidonaphthalen-6-yl) propane-1,3-diamine, MitoBlue, which selectively stains functional mitochondria while displaying low toxicity, bright blue emission, and high resistance to photobleaching. Additionally, we show that a biotin-labeled MitoBlue derivative can be used as a handle for the delivery of streptavidin-tagged species to the mitochondria.
authors.affiliation: Departamento de Química Orgánica and Centro Singular de Investigación en Química Biolóxica e Materiais Moleculares (CIQUS), and ‡Departamento de Bioquímica y Biología Molecular and Centro Singular de Investigación en Química Biolóxica e Materiais Moleculares (CIQUS), Universidade de Santiago de Compostela , 15782 Santiago de Compostela, Spain.
authors.forename: Mateo I
authors.initials: MI
authors.lastname: Sánchez
authors.forename: José
authors.initials: J
authors.lastname: Martínez-Costas
authors.forename: José L
authors.initials: JL
authors.lastname: Mascareñas
authors.forename: M Eugenio
authors.initials: ME
authors.lastname: Vázquez
mesh_terms: D015081:2-Naphthylamine / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry; D000578:Amidines / Q000138:chemical synthesis / Q000737:chemistry*; D000818:Animals; D001710:Biotin / Q000737:chemistry; D002645:Chickens; D002522:Chlorocebus aethiops; D004625:Embryo, Nonmammalian; D005347:Fibroblasts / Q000502:physiology / Q000648:ultrastructure*; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D008856:Microscopy, Fluorescence; D008928:Mitochondria / Q000502:physiology / Q000648:ultrastructure*; D013194:Staining and Labeling; D019809:Streptavidin / Q000737:chemistry; D014709:Vero Cells
chemical_list: D000578:Amidines; D005456:Fluorescent Dyes; C000600442:MitoBlue; D001710:Biotin; D019809:Streptavidin; D015081:2-Naphthylamine