title: Chemical characterization of a red raspberry fruit extract and evaluation of its pharmacological effects in experimental models of acute inflammation and collagen-induced arthritis.
abstract: Berries are an important dietary source of fibres, vitamins, minerals and some biologically active non-nutrients. A red raspberry fruit extract was characterized in terms of phenolic content and the anti-inflammatory properties and protective effects were evaluated in two experimental models of inflammation. The antioxidant potential of the extract, the cellular antioxidant activity and the effects over neutrophils' oxidative burst were also studied to provide a mechanistic insight for the anti-inflammatory effects observed. The extract was administered in a dose of 15 mg kg(-1), i.p. and significantly inhibited paw oedema formation in the rat. The same dose was administered via i.p. and p.o. routes in the collagen-induced arthritis model in the rat. The extract showed pharmacological activity and was able to significantly reduce the development of clinical signs of arthritis and markedly reduce the degree of bone resorption, soft tissue swelling and osteophyte formation, preventing articular destruction in treated animals.
authors.affiliation: iMED.ULisboa, Faculdade de Farmácia da Universidade de Lisboa, Avenida Prof. Gama Pinto, 1649-003 Lisboa, Portugal. bsepodes@ff.ul.pt.
authors.forename: M E
authors.initials: ME
authors.lastname: Figueira
authors.forename: M B
authors.initials: MB
authors.lastname: Câmara
authors.forename: R
authors.initials: R
authors.lastname: Direito
authors.forename: J
authors.initials: J
authors.lastname: Rocha
authors.forename: A T
authors.initials: AT
authors.lastname: Serra
authors.forename: C M M
authors.initials: CM
authors.lastname: Duarte
authors.forename: A
authors.initials: A
authors.lastname: Fernandes
authors.forename: M
authors.initials: M
authors.lastname: Freitas
authors.forename: E
authors.initials: E
authors.lastname: Fernandes
authors.forename: M C
authors.initials: MC
authors.lastname: Marques
authors.forename: M R
authors.initials: MR
authors.lastname: Bronze
authors.forename: B
authors.initials: B
authors.lastname: Sepodes
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage* / Q000737:chemistry*; D000975:Antioxidants / Q000008:administration & dosage / Q000737:chemistry; D001168:Arthritis / Q000188:drug therapy* / Q000276:immunology; D004195:Disease Models, Animal; D004353:Drug Evaluation, Preclinical; D004487:Edema / Q000188:drug therapy* / Q000276:immunology; D005638:Fruit / Q000737:chemistry; D006801:Humans; D008297:Male; D009504:Neutrophils / Q000187:drug effects / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D010936:Plant Extracts / Q000008:administration & dosage* / Q000737:chemistry*; D051381:Rats; D017208:Rats, Wistar; D027824:Rosaceae / Q000737:chemistry*
chemical_list: D000893:Anti-Inflammatory Agents; D000975:Antioxidants; D010936:Plant Extracts