title: Effects of supplementation with two sources and two levels of copper on meat lipid oxidation, meat colour and superoxide dismutase and glutathione peroxidase enzyme activities in Nellore beef cattle.
abstract: In the present study, thirty-five Nellore bulls were used to determine the effects of two levels and two sources (organic and inorganic) of Cu supplementation on the oxidative stability of lipids, measured by the thiobarbituric acid-reactive substance (TBARS) test, meat colour and superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) enzyme activities. The following treatments were used: (1) control (C) - basal diet without supplementation of Cu (7 mg Cu/kg DM); (2) I10 - basal diet supplemented with 10 mg Cu/kg DM in the form of copper sulphate (inorganic form); (3) I40 - basal diet supplemented with 40 mg Cu/kg DM in the form of copper sulphate; (4) O10 - basal diet supplemented with 10 mg Cu/kg DM in the form of copper proteinate (organic form); (5) O40 - basal diet supplemented with 40 mg Cu/kg DM in the form of copper proteinate. Lipid oxidation was determined in meat samples exposed to display, modified atmosphere (MA) and vacuum packaging (VC) conditions and in liver samples using the TBARS test. These samples were also evaluated for meat discolouration after exposure to air. The activities of SOD and GSH-Px enzymes were determined in liver samples. In display, MA and VC conditions, the TBARS values of samples from animals supplemented with 40 mg Cu/kg DM were lower than those of samples from control animals. There was no effect of treatment on the colour variables (L*, a*, b*). There was also no significant effect of treatment on hepatic TBARS concentrations and GSH-Px activity. Supplementation with Cu at 40 mg/kg, regardless of the source, induced higher hepatic SOD activity compared with the control treatment. In conclusion, Cu supplementation improved the oxidative stability of lipids in samples exposed to display, MA and VC conditions, demonstrating the antioxidant effect of this mineral.
authors.affiliation: College of Animal Science and Food Engineering (FZEA), University of São Paulo (USP),Avenida Duque de Caxias Norte, 225,CEP 13635-900,Pirassununga,SP,Brazil.
authors.forename: Lísia Bertonha
authors.initials: LB
authors.lastname: Correa
authors.affiliation: College of Animal Science and Food Engineering (FZEA), University of São Paulo (USP),Avenida Duque de Caxias Norte, 225,CEP 13635-900,Pirassununga,SP,Brazil.
authors.forename: Marcus Antonio
authors.initials: MA
authors.lastname: Zanetti
authors.affiliation: College of Animal Science and Food Engineering (FZEA), University of São Paulo (USP),Avenida Duque de Caxias Norte, 225,CEP 13635-900,Pirassununga,SP,Brazil.
authors.forename: Gustavo Ribeiro
authors.initials: GR
authors.lastname: Del Claro
authors.affiliation: Federal Institute of Education, Science and Technology of Rio Grande do Sul (IFRS-Sertão),Rio Grande do Sul,Brazil.
authors.forename: Fernanda Alves
authors.initials: FA
authors.lastname: de Paiva
authors.affiliation: College of Animal Science and Food Engineering (FZEA), University of São Paulo (USP),Avenida Duque de Caxias Norte, 225,CEP 13635-900,Pirassununga,SP,Brazil.
authors.forename: Saulo
authors.initials: S
authors.lastname: da Luz e Silva
authors.affiliation: College of Animal Science and Food Engineering (FZEA), University of São Paulo (USP),Avenida Duque de Caxias Norte, 225,CEP 13635-900,Pirassununga,SP,Brazil.
authors.forename: Arlindo Saran
authors.initials: AS
authors.lastname: Netto
mesh_terms: D000818:Animals; D017849:Animals, Inbred Strains; D000975:Antioxidants / Q000008:administration & dosage / Q000009:adverse effects / Q000627:therapeutic use; D001938:Brazil; D002417:Cattle; D003300:Copper / Q000008:administration & dosage / Q000009:adverse effects / Q000172:deficiency / Q000627:therapeutic use*; D003677:Deficiency Diseases / Q000178:diet therapy / Q000662:veterinary*; D004032:Diet / Q000009:adverse effects / Q000662:veterinary*; D018857:Food Packaging; D063427:Food Quality; D061353:Food Storage; D005979:Glutathione Peroxidase / Q000378:metabolism; D015227:Lipid Peroxidation; D008099:Liver / Q000201:enzymology* / Q000378:metabolism; D008297:Male; D008460:Meat / Q000032:analysis*; D018482:Muscle, Skeletal / Q000378:metabolism*; D010858:Pigmentation; D013482:Superoxide Dismutase / Q000737:chemistry / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
chemical_list: D000975:Antioxidants; D017392:Thiobarbituric Acid Reactive Substances; D003300:Copper; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase