title: Neuropeptide Y and its C-terminal fragments acting on Y2 receptor: Raman and SERS spectroscopy studies.
abstract: In this paper, we present spectroscopic studies of neuropeptide Y (NPY) and its native NPY(3-36), NPY(13-36), and NPY(22-36) and mutated acetyl-(Leu(28,31))-NPY(24-36)C-terminal fragments acting on Y2 receptor. Since there is some evidence for the correlation between the SERS patterns and the receptor binding ability, we performed a detailed analysis for these compounds at the metal/water interface using Raman spectroscopy (RS) and surface-enhanced Raman spectroscopy (SERS) methods. Many studies have suggested that interactions of this kind are crucial for a variety of biomedical and biochemical phenomena. The identification of amino acids in these peptide sequences by SERS allowed us to determine which molecular fragments were responsible for the interaction with the silver nanoparticle surface. Our findings demonstrated that in all of the investigated compounds, the NPY(32-36)C-terminal fragment (Thr(32)-Arg(33)-Gln(34)-Arg(35)-Tyr(36)NH2) was involved in the adsorption process onto metal substrate. The results of the present study suggest that the same molecular fragment interacts with the Y2 receptor, what proved the usefulness of the SERS method in the study of these biologically active compounds. The search for analogs acting on Y2 receptor may be important from the viewpoint of possible future clinical applications.
authors.affiliation: Department of Neurobiology, Institute of Pharmacology, Polish Academy of Sciences, Smętna 12, 31-343 Kraków, Poland.
authors.forename: Helena
authors.initials: H
authors.lastname: Domin
authors.affiliation: Faculty of Chemistry, Jagiellonian University, ul. Ingardena 3, 30-060 Kraków, Poland.
authors.forename: Ewa
authors.initials: E
authors.lastname: Pięta
authors.affiliation: Faculty of Chemistry, Jagiellonian University, ul. Ingardena 3, 30-060 Kraków, Poland.
authors.forename: Natalia
authors.initials: N
authors.lastname: Piergies
authors.affiliation: Faculty of Chemistry, Jagiellonian University, ul. Ingardena 3, 30-060 Kraków, Poland.
authors.forename: Dominika
authors.initials: D
authors.lastname: Święch
authors.affiliation: Department of Chemistry, Hankuk University of Foreign Studies, Yongin, Kyunggi-Do 449-791, Republic of Korea.
authors.forename: Younkyoo
authors.initials: Y
authors.lastname: Kim
authors.affiliation: Faculty of Chemistry, Jagiellonian University, ul. Ingardena 3, 30-060 Kraków, Poland.
authors.forename: Leonard M
authors.initials: LM
authors.lastname: Proniewicz
authors.affiliation: Faculty of Foundry Engineering, AGH University of Science and Technology, ul. Reymonta 23, 30-059 Kraków, Poland. Electronic address: proniewi@agh.edu.pl.
authors.forename: Edyta
authors.initials: E
authors.lastname: Proniewicz
mesh_terms: D000818:Animals; D006801:Humans; D009478:Neuropeptide Y / Q000737:chemistry* / Q000494:pharmacology; D017476:Receptors, Neuropeptide Y / Q000187:drug effects*; D013056:Spectrophotometry, Ultraviolet; D013059:Spectrum Analysis, Raman / Q000379:methods*
chemical_list: D009478:Neuropeptide Y; D017476:Receptors, Neuropeptide Y; C095889:neuropeptide Y2 receptor
keywords: Native and mutated C-terminal NPY fragments; Neuropeptide Y, NPY; Raman spectroscopy, RS; Silver colloid; Surface-enhanced Raman Spectroscopy, SERS; Y(2) receptor agonist