title: Identification of novel microRNAs in the Verticillium wilt-resistant upland cotton variety KV-1 by high-throughput sequencing.
abstract: Plant microRNAs (miRNAs) play essential roles in the post-transcriptional regulation of gene expression during development, flowering, plant growth, metabolism, and stress responses. Verticillium wilt is one of the vascular disease in plants, which is caused by the Verticillium dahlia and leads to yellowing, wilting, lodging, damage to the vascular tissue, and death in cotton plants. Upland cotton varieties KV-1 have shown resistance to Verticillium wilt in multiple levels. However, the knowledge regarding the post-transcriptional regulation of the resistance is limited. Here two novel small RNA (sRNA) libraries were constructed from the seedlings of upland cotton variety KV-1, which is highly resistant to Verticillium wilts and inoculated with the V991 and D07038 Verticillium dahliae (V. dahliae) of different virulence strains. Thirty-seven novel miRNAs were identified after sequencing these two libraries by the Illumina Solexa system. According to sequence homology analysis, potential target genes of these miRNAs were predicted. With no more than three sequence mismatches between the novel miRNAs and the potential target mRNAs, we predicted 49 target mRNAs for 24 of the novel miRNAs. These target mRNAs corresponded to genes were found to be involved in plant-pathogen interactions, endocytosis, the mitogen-activated protein kinase (MAPK) signaling pathway, and the biosynthesis of isoquinoline alkaloid, terpenoid backbone, primary bile acid and secondary metabolites. Our results showed that some of these miRNAs and their relative gene are involved in resistance to Verticillium wilts. The identification and characterization of miRNAs from upland cotton could help further studies on the miRNA regulatory mechanisms of resistance to Verticillium wilt.
authors.affiliation: College of Bioinformation, Chongqing University of Posts and Telecommunications, Chongqing, 400065 China.
authors.forename: Xiaohong
authors.initials: X
authors.lastname: He
authors.affiliation: College of Bioinformation, Chongqing University of Posts and Telecommunications, Chongqing, 400065 China.
authors.forename: Quan
authors.initials: Q
authors.lastname: Sun
authors.affiliation: College of Bioinformation, Chongqing University of Posts and Telecommunications, Chongqing, 400065 China.
authors.forename: Huaizhong
authors.initials: H
authors.lastname: Jiang
authors.affiliation: College of Bioinformation, Chongqing University of Posts and Telecommunications, Chongqing, 400065 China.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Zhu
authors.affiliation: State Key Laboratory of Cotton Biology, Henan Key Laboratory of Plant Stress Biology, College of Life Sciences, Henan University, Kaifeng, 475004 China.
authors.forename: Jianchuan
authors.initials: J
authors.lastname: Mo
authors.affiliation: State Key Laboratory of Cotton Biology, Henan Key Laboratory of Plant Stress Biology, College of Life Sciences, Henan University, Kaifeng, 475004 China.
authors.forename: Lu
authors.initials: L
authors.lastname: Long
authors.affiliation: College of Bioinformation, Chongqing University of Posts and Telecommunications, Chongqing, 400065 China.
authors.forename: Liuxin
authors.initials: L
authors.lastname: Xiang
authors.affiliation: College of Bioinformation, Chongqing University of Posts and Telecommunications, Chongqing, 400065 China.
authors.forename: Yongfang
authors.initials: Y
authors.lastname: Xie
authors.affiliation: State Key Laboratory of Cotton Biology, Cotton Institute of the Chinese Academy of Agricultural Sciences, Key Laboratory of Cotton Genetic Improvement, Ministry of Agriculture, Anyang, Henan 455000 China.
authors.forename: Yuzhen
authors.initials: Y
authors.lastname: Shi
authors.affiliation: State Key Laboratory of Cotton Biology, Cotton Institute of the Chinese Academy of Agricultural Sciences, Key Laboratory of Cotton Genetic Improvement, Ministry of Agriculture, Anyang, Henan 455000 China.
authors.forename: Youlu
authors.initials: Y
authors.lastname: Yuan
authors.affiliation: State Key Laboratory of Cotton Biology, Henan Key Laboratory of Plant Stress Biology, College of Life Sciences, Henan University, Kaifeng, 475004 China ; College of Bioinformation, Chongqing University of Posts and Telecommunications, Chongqing, 400065 China.
authors.forename: Yingfan
authors.initials: Y
authors.lastname: Cai
keywords: Deep sequencing; Gossypium hirsutum; KV-1; MicroRNAs; Verticillium dahliae Kleb