title: Characterization of white spot syndrome virus VP52B and its interaction with VP26.
abstract: White spot syndrome virus (WSSV) is one of the major pathogens of cultured shrimp. Identification of envelope protein interactions has become a central issue for the understanding of WSSV assembly. In this paper, WSSV envelope protein VP52B was fused with GST-tag and expressed in Escherichia coli BL-21(DE3). Immunogold-electron microscopy revealed that VP52B was located on the outside surface of WSSV virions. Far-Western blotting analysis suggested that VP52B might directly interact with a major viral envelope protein VP26, and their interaction was confirmed by GST pull-down assay. Further investigation showed that the VP52B binding domain was located between residues 135-170 of VP26. These findings will enhance our understanding of the molecular mechanisms of WSSV morphogenesis.
authors.affiliation: State Key Laboratory Breeding Base of Marine Genetic Resources, Key Laboratory of Marine Genetic Resources of State Oceanic Administration, Third Institute of Oceanography, Xiamen, 361005, People's Republic of China.
authors.forename: Fanyu
authors.initials: F
authors.lastname: Lin
authors.forename: Zuliang
authors.initials: Z
authors.lastname: Jie
authors.forename: Luhong
authors.initials: L
authors.lastname: Hou
authors.forename: Fang
authors.initials: F
authors.lastname: Li
authors.forename: Feng
authors.initials: F
authors.lastname: Yang
mesh_terms: D026481:Blotting, Far-Western; D002498:Centrifugation; D016253:Microscopy, Immunoelectron; D025941:Protein Interaction Mapping; D014759:Viral Envelope Proteins / Q000378:metabolism*; D014771:Virion / Q000737:chemistry; D046848:White spot syndrome virus 1 / Q000737:chemistry / Q000502:physiology*
chemical_list: D014759:Viral Envelope Proteins