title: Characterization of the novel Sezary lymphoma cell line BKP1.
abstract: Cutaneous T-cell lymphomas (CTCL) are a heterogeneous group of lymphomas primarily involving the skin. The most common types are mycosis fungoides (MF) and Sezary Syndrome (SS). We report a novel long-term fast-growing SS line termed BKP1 that was characterized by flow cytometry (FC), conventional and molecular cytogenetic [FISH/multi-FISH together with array comparative genomic hybridization (aCGH)]. FC immunophenotype of the BKP1 is CD2+CD5+CD3+CD4+CD8-CD7-CD25-CD26-CD30-CD158k+. The TCRγ characterization of BKP1 by PCR identified a clonal rearrangement. The conventional cytogenetic and Multi-FISH analysis showed complex chromosomal rearrangements. aCGH analysis highlighted the loss of genes involved in cell cycle control, in immune response (HLA, complement complex) and DNA damage repair mechanisms. The BKP1 is another lymphoma cell line thoroughly characterized that can be a valuable tool for both basic and applied research such as identification of deregulated genes and/or pathways and screening for new antilymphoma drugs.
authors.affiliation: Département de Génétique Médicale, Hôpital La Timone, Assistance Publique-Hôpitaux de Marseille, Marseille, France; Aix-Marseille Université, Marseille, France.
authors.forename: John
authors.initials: J
authors.lastname: Boudjarane
authors.forename: Arnaud
authors.initials: A
authors.lastname: Essaydi
authors.forename: Laure
authors.initials: L
authors.lastname: Farnault
authors.forename: Cornel
authors.initials: C
authors.lastname: Popovici
authors.forename: Marina
authors.initials: M
authors.lastname: Lafage-Pochitaloff
authors.forename: Nathalie
authors.initials: N
authors.lastname: Beaufils
authors.forename: Yaël
authors.initials: Y
authors.lastname: Berda-Haddad
authors.forename: Romaric
authors.initials: R
authors.lastname: Lacroix
authors.forename: Corinne
authors.initials: C
authors.lastname: Nicolino-Brunet
authors.forename: Thérèse
authors.initials: T
authors.lastname: Le Treut
authors.forename: Hélène
authors.initials: H
authors.lastname: Zattara
authors.forename: Jean
authors.initials: J
authors.lastname: Gabert
authors.forename: Brigitte
authors.initials: B
authors.lastname: Kahn-Perlès
authors.forename: Régis
authors.initials: R
authors.lastname: Costello
mesh_terms: D001706:Biopsy; D045744:Cell Line, Tumor; D002869:Chromosome Aberrations; D002875:Chromosomes / Q000648:ultrastructure; D005434:Flow Cytometry; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D016130:Immunophenotyping; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D016410:Lymphoma, T-Cell, Cutaneous / Q000235:genetics* / Q000473:pathology*; D012751:Sezary Syndrome / Q000235:genetics* / Q000473:pathology*; D012867:Skin / Q000473:pathology
keywords: T-cell lymphoma; cutaneous lymphoma; karyotype