title: Metabolic oxygen consumption measurement with a single-cell biosensor after particle microbeam irradiation.
abstract: A noninvasive, self-referencing biosensor/probe system has been integrated into the Columbia University Radiological Research Accelerator Facility Microbeam II end station. A single-cell oxygen consumption measurement has been conducted with this type of oxygen probe in 37° C Krebs-Ringer Bicarbonate buffer immediately before and after a single-cell microbeam irradiation. It is the first such measurement made for a microbeam irradiation, and a six fold increment of oxygen flux induced during a 15-s period of time has been observed following radiation exposure. The experimental procedure and the results are discussed.
authors.affiliation: Radiological Research Accelerator Facility (RARAF), Center for Radiological Research, Columbia University, 136 S. Broadway, Irvington, NY, 10533, USA. yx2132@columbia.edu.
authors.forename: Yanping
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Center for Radiological Research, Columbia University, New York, NY, 10032, USA.
authors.forename: Bo
authors.initials: B
authors.lastname: Zhang
authors.affiliation: Biocurrents Research Center, Marine Biological Laboratory, Woods Hole, MA, 02543, USA.|Department of Biology and Microbiology, South Dakota State University, Brookings, SD, 57007, USA.
authors.forename: Mark
authors.initials: M
authors.lastname: Messerli
authors.affiliation: Radiological Research Accelerator Facility (RARAF), Center for Radiological Research, Columbia University, 136 S. Broadway, Irvington, NY, 10533, USA.
authors.forename: Gerhard
authors.initials: G
authors.lastname: Randers-Pehrson
authors.affiliation: Center for Radiological Research, Columbia University, New York, NY, 10032, USA.
authors.forename: Tom K
authors.initials: TK
authors.lastname: Hei
authors.affiliation: Radiological Research Accelerator Facility (RARAF), Center for Radiological Research, Columbia University, 136 S. Broadway, Irvington, NY, 10533, USA.
authors.forename: David J
authors.initials: DJ
authors.lastname: Brenner
mesh_terms: D015374:Biosensing Techniques; D002460:Cell Line; D004566:Electrodes; D006801:Humans; D010101:Oxygen Consumption; D010315:Particle Accelerators; D011853:Radiobiology / Q000295:instrumentation