title: Increased expression of IRS-1 is associated with lymph node metastasis in nasopharyngeal carcinoma.
abstract: Nasopharyngeal carcinoma (NPC) is a head and neck malignant tumor rare throughout most of the world but common in Southeast Asia, especially in Southern China, which is with characteristics of early cervical lymph node metastasis and high incidence rate of distant metastasis. Insulin receptor substrate 1 (IRS-1) is a signaling adapter protein that is encoded by the IRS-1 gene in humans, plays an important role in the development, progression, invasion and metastasis of tumors. The aim of the present study was to investigate the association between the expression of IRS-1 protein and clinicopathological characteristics in NPC by immunohistochemistry. The results showed that the expression level of IRS-1 was significant higher in NPC than that in the control nasopharyngeal epithelia (P = 0.042). The positive percentage of IRS-1 expression in NPC with lymph node metastasis was also significantly higher than those without lymph node metastasis (P = 0.008). Positive expression of IRS-1 was proved to be the independent predicted factor for lymph node metastasis of NPC (P = 0.025) regardless of age, gender, histological type and clinical stages by multivariate logistic regression analysis. In addition, results showed higher sensitivity and agreement rate of IRS-1 for predicting lymph node metastasis of NPC patients. Taken together, high expression of IRS-1 might be closely correlated with lymph node metastasis in NPC and positive expression of IRS-1 could be used as an independent biomarker for predicting lymph node metastasis of NPC.
authors.affiliation: Department of Pathology, The Second Xiangya Hospital, Central South University Changsha, Hunan, China.
authors.forename: Jiadi
authors.initials: J
authors.lastname: Luo
authors.affiliation: Department of Pathology, The Second Xiangya Hospital, Central South University Changsha, Hunan, China.
authors.forename: Qiuyuan
authors.initials: Q
authors.lastname: Wen
authors.affiliation: Department of Pathology, The Second Xiangya Hospital, Central South University Changsha, Hunan, China.
authors.forename: Jiao
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Pathology, The Second Xiangya Hospital, Central South University Changsha, Hunan, China.
authors.forename: Lina
authors.initials: L
authors.lastname: Xu
authors.affiliation: Department of Pathology, The Second Xiangya Hospital, Central South University Changsha, Hunan, China.
authors.forename: Shuzhou
authors.initials: S
authors.lastname: Chu
authors.affiliation: Department of Pathology, The Second Xiangya Hospital, Central South University Changsha, Hunan, China.
authors.forename: Weiyuan
authors.initials: W
authors.lastname: Wang
authors.affiliation: Department of Pathology, The Second Xiangya Hospital, Central South University Changsha, Hunan, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Shi
authors.affiliation: Department of Oncology, The Second Xiangya Hospital, Central South University Changsha, Hunan, China.
authors.forename: Guiyuan
authors.initials: G
authors.lastname: Xie
authors.affiliation: Department of Otorhinolaryngology, Xiangya Hospital, Central South University Changsha, Hunan, China.
authors.forename: Donghai
authors.initials: D
authors.lastname: Huang
authors.affiliation: Department of Pathology, The Second Xiangya Hospital, Central South University Changsha, Hunan, China.
authors.forename: Songqing
authors.initials: S
authors.lastname: Fan
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000032:analysis*; D002277:Carcinoma / Q000737:chemistry* / Q000401:mortality / Q000556:secondary* / Q000628:therapy; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D055504:Insulin Receptor Substrate Proteins / Q000032:analysis*; D053208:Kaplan-Meier Estimate; D016015:Logistic Models; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D015999:Multivariate Analysis; D000077274:Nasopharyngeal Carcinoma; D009303:Nasopharyngeal Neoplasms / Q000737:chemistry* / Q000401:mortality / Q000473:pathology* / Q000628:therapy; D009367:Neoplasm Staging; D011237:Predictive Value of Tests; D012307:Risk Factors; D015854:Up-Regulation; D055815:Young Adult
chemical_list: D014408:Biomarkers, Tumor; C526292:IRS1 protein, human; D055504:Insulin Receptor Substrate Proteins
keywords: Insulin receptor substrate (IRS); biomarker; insulin receptor substrate 1 (IRS-1); metastasis; nasopharyngeal carcinoma (NPC)