title: Ultrasensitive immunoassay based on a pseudobienzyme amplifying system of choline oxidase and luminol-reduced Pt@Au hybrid nanoflowers.
abstract: A multi-functional luminol-reduced Pt@Au hybrid flower-like nanocomposite (luminol-Pt@AuNF) which not only acts as an efficient signal probe but also constitutes a pseudobienzyme amplifying system with choline oxidase (ChOx) was firstly synthesized and applied to the construction of a solid-state luminol electrochemiluminescence (ECL) immunosensor for cardiac troponin I (cTnI) detection.
authors.affiliation: Key Laboratory of Luminescent and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China. yuanruo@swu.edu.cn yqchai@swu.edu.cn.
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhou
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhuo
authors.forename: Ni
authors.initials: N
authors.lastname: Liao
authors.forename: Yaqin
authors.initials: Y
authors.lastname: Chai
authors.forename: Ruo
authors.initials: R
authors.lastname: Yuan
mesh_terms: D000429:Alcohol Oxidoreductases / Q000737:chemistry* / Q000378:metabolism*; D000497:Alloys / Q000737:chemistry* / Q000378:metabolism; D015415:Biomarkers / Q000097:blood; D055664:Electrochemical Techniques; D006046:Gold / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D007118:Immunoassay; D008163:Luminescent Measurements; D008165:Luminol / Q000737:chemistry / Q000378:metabolism*; D049329:Nanostructures / Q000737:chemistry*; D010084:Oxidation-Reduction; D010984:Platinum / Q000737:chemistry / Q000378:metabolism; D019210:Troponin I / Q000097:blood*
chemical_list: D000497:Alloys; D015415:Biomarkers; D019210:Troponin I; D010984:Platinum; D008165:Luminol; D006046:Gold; D000429:Alcohol Oxidoreductases; C019888:choline oxidase