title: Suppressor of cytokine signaling 1 counteracts rhesus macaque TRIM5α-induced inhibition of human immunodeficiency virus type-1 production.
abstract: Old world monkey TRIM5α is a host factor that restricts human immunodeficiency virus type-1 (HIV-1) infection. Previously, we reported that rhesus macaque TRIM5α (RhTRIM5α) restricts HIV-1 production by inducing degradation of precursor Gag. Since suppressor of cytokine signaling 1 (SOCS1) is known to enhance HIV-1 production by rescuing Gag from lysosomal degradation, we examined if SOCS1 is involved in RhTRIM5α-mediated late restriction. Over-expression of SOCS1 restored HIV-1 production in the presence of RhTRIM5α to a level comparable to that in the absence of RhTRIM5α in terms of titer and viral protein expression. Co-immunoprecipitation studies revealed that SOCS1 physically interacted with RhTRIM5α. Over-expression of SOCS1 affected RhTRIM5α expression in a dose-dependent manner, which was not reversed by proteasome inhibitors. In addition, SOCS1 and RhTRIM5α were detected in virus-like particles. These results suggest that SOCS1 alleviates RhTRIM5α-mediated regulation in the late phase of HIV-1 life cycle probably due to the destabilization of RhTRIM5α.
authors.affiliation: Department of Molecular Virology, Tokyo Medical and Dental University, Tokyo, Japan.
authors.forename: Sayaka
authors.initials: S
authors.lastname: Sukegawa
authors.affiliation: Department of Molecular Virology, Tokyo Medical and Dental University, Tokyo, Japan.
authors.forename: Ryuta
authors.initials: R
authors.lastname: Sakuma
authors.affiliation: Department of Molecular Medicine, Mayo Clinic, Rochester, Minnesota, United States of America.
authors.forename: Seiga
authors.initials: S
authors.lastname: Ohmine
authors.affiliation: Department of Molecular Virology, Tokyo Medical and Dental University, Tokyo, Japan.
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Takeuchi
authors.affiliation: Department of Molecular Medicine, Mayo Clinic, Rochester, Minnesota, United States of America.
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Ikeda
authors.affiliation: Department of Molecular Virology, Tokyo Medical and Dental University, Tokyo, Japan.
authors.forename: Shoji
authors.initials: S
authors.lastname: Yamaoka
mesh_terms: D000818:Animals; D002460:Cell Line; D057809:HEK293 Cells; D015497:HIV-1 / Q000502:physiology*; D006801:Humans; D008253:Macaca mulatta; D011506:Proteins / Q000037:antagonists & inhibitors / Q000378:metabolism*; D050826:Suppressor of Cytokine Signaling Proteins / Q000378:metabolism*; D044767:Ubiquitin-Protein Ligases; D014779:Virus Replication
chemical_list: D011506:Proteins; D050826:Suppressor of Cytokine Signaling Proteins; C482587:TRIM5(alpha) protein, rhesus monkey; D044767:Ubiquitin-Protein Ligases