title: [Laser scanning confocal microscopic imaging for Ca2 + oscillations of pancreatic acinar cells in mice].
abstract: OBJECTIVE
  To establish a simple but effective method of laser scanning confocal microscopic imaging for Ca2+ oscillations of pancreatic acinar cells in adult mice.
  
  
  METHODS
  Pancreatic acinar cells from adult Kunming mice were isolated acutely with collagenase, and then loaded with fluo-4-AM, a Ca2+ indicator. A laser scanning confocal microscope armed with 488 nm laser was employed to record the dynamic fluorescent signals in-time and synchronously while acetylcholine (ACh) was added in the pancreatic acinar cells.
  
  
  RESULTS
  (1) The classic pancreatic acinar cell Ca2+ oscillations were induced by a certain concentration of ACh (100 nmol/L) successfully and steadily, which could be blocked by atropine completely. (2) Plasmic Ca2+ oscillations from different parts of one acinar cell were usually with different amplitudes and almost the same frequencies. But both of amplitudes and frequencies were different among different cells. (3) The acinar cell Ca2+ oscillations were induced by ACh in a concentration-dependent manner.
  
  
  CONCLUSIONS
  The laser scanning confocal microscopic imaging for adult mouse pancreatic acinar cell Ca2+ oscillations was established successfully. The features of being easy to use, direct to see lively, high efficiency and good flexibility make it a popular tool for researchers to choose.
authors.forename: Jing-Ke
authors.initials: JK
authors.lastname: Wang
authors.forename: Meng-Qin
authors.initials: MQ
authors.lastname: Zhao
authors.forename: Na-Na
authors.initials: NN
authors.lastname: Sun
authors.forename: Fang-Fan
authors.initials: FF
authors.lastname: Sun
authors.forename: Jie
authors.initials: J
authors.lastname: Wu
authors.forename: Jian-Xin
authors.initials: JX
authors.lastname: Shen
authors.forename: Hai-Yan
authors.initials: HY
authors.lastname: Wang
mesh_terms: D061354:Acinar Cells / Q000737:chemistry*; D000818:Animals; D002118:Calcium / Q000032:analysis*; D020013:Calcium Signaling; D002478:Cells, Cultured; D051379:Mice; D018613:Microscopy, Confocal / Q000379:methods*; D010179:Pancreas / Q000166:cytology*
chemical_list: D002118:Calcium