title: Detection of p53 protein transcriptional activity by chromatin immunoprecipitation.
abstract: p53 is a key transcriptional mediator that controls the expression of hundreds of target genes necessary to maintain cellular homeostasis and genome integrity. An important cellular function that is dependent on p53 transcriptional activity is apoptosis or programmed cell death. Indeed, inhibition of p53 transcriptional activity is often observed in cancers as a result of mutations within its DNA-binding domain. In this chapter, we describe the use of chromatin immunoprecipitation and real-time quantitative polymerase chain reaction to detect p53 transcriptional activity in cancer cells and tumor tissues. This technique enables the determination of the ability of p53 to bind to the promoter region of apoptotic genes and to evaluate the transcription-dependent activity of p53-induced apoptosis.
authors.affiliation: Department of Obstetrics, Gynecology and Reproductive Sciences, Yale University School of Medicine, New Haven, CT, USA.
authors.forename: Yang
authors.initials: Y
authors.lastname: Yang-Hartwich
authors.forename: Emily
authors.initials: E
authors.lastname: Romanoff
authors.forename: Jamie
authors.initials: J
authors.lastname: Bingham
authors.forename: Ayesha B
authors.initials: AB
authors.lastname: Alvero
authors.forename: Gil
authors.initials: G
authors.lastname: Mor
mesh_terms: D047369:Chromatin Immunoprecipitation / Q000379:methods*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D009369:Neoplasms / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods; D014158:Transcription, Genetic; D016159:Tumor Suppressor Protein p53 / Q000032:analysis / Q000235:genetics / Q000378:metabolism*
chemical_list: D016159:Tumor Suppressor Protein p53