title: Identification of bacterial and fungal pathogens from positive blood culture bottles: a microarray-based approach.
abstract: Rapid identification and characterization of bacterial and fungal pathogens present in the bloodstream are essential for optimal patient management and are associated with improved patient outcomes, improved antimicrobial stewardship, improved infection control, and reduced healthcare costs. Microarrays serve as reliable platforms for the identification of these bloodstream pathogens and their associated antimicrobial resistance genes, if present. Nanosphere's (Nanosphere, Inc., Northbrook, IL, USA) Verigene Gram-Positive Blood Culture Nucleic-Acid Test (BC-GP) is one such microarray-based approach for the detection of bacteria that cause bloodstream infection. Here, we describe the design of the microarray-based Verigene BC-GP Test, the steps necessary for performing the test, and the different components of the test including nucleic acid extraction and hybridization of target nucleic acid to a microarray.
authors.affiliation: Nanosphere Inc., 4088 Commercial Avenue, Northbrook, 60062, IL, USA.
authors.forename: Teresa
authors.initials: T
authors.lastname: Raich
authors.forename: Scott
authors.initials: S
authors.lastname: Powell
mesh_terms: D057205:Automation, Laboratory; D016470:Bacteremia / Q000175:diagnosis* / Q000382:microbiology / Q000473:pathology; D001419:Bacteria / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification*; D015373:Bacterial Typing Techniques / Q000295:instrumentation / Q000379:methods*; D001800:Blood Specimen Collection; D003470:Culture Media / Q000737:chemistry; D004269:DNA, Bacterial / Q000235:genetics*; D024881:Drug Resistance, Bacterial / Q000235:genetics; D006801:Humans; D046228:Microarray Analysis / Q000295:instrumentation / Q000379:methods*; D009693:Nucleic Acid Hybridization; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity
chemical_list: D003470:Culture Media; D004269:DNA, Bacterial; D011933:Reagent Kits, Diagnostic