title: Early and delayed long-term transcriptional changes and short-term transient responses during cold acclimation in olive leaves.
abstract: Low temperature severely affects plant growth and development. To overcome this constraint, several plant species from regions having a cool season have evolved an adaptive response, called cold acclimation. We have studied this response in olive tree (Olea europaea L.) cv. Picual. Biochemical stress markers and cold-stress symptoms were detected after the first 24 h as sagging leaves. After 5 days, the plants were found to have completely recovered. Control and cold-stressed plants were sequenced by Illumina HiSeq 1000 paired-end technique. We also assembled a new olive transcriptome comprising 157,799 unigenes and found 6,309 unigenes differentially expressed in response to cold. Three types of response that led to cold acclimation were found: short-term transient response, early long-term response, and late long-term response. These subsets of unigenes were related to different biological processes. Early responses involved many cold-stress-responsive genes coding for, among many other things, C-repeat binding factor transcription factors, fatty acid desaturases, wax synthesis, and oligosaccharide metabolism. After long-term exposure to cold, a large proportion of gene down-regulation was found, including photosynthesis and plant growth genes. Up-regulated genes after long-term cold exposure were related to organelle fusion, nucleus organization, and DNA integration, including retrotransposons.
authors.affiliation: Departamento de Biología Experimental, Universidad de Jaén, Jaén, Spain.
authors.forename: María de la O
authors.initials: Mde L
authors.lastname: Leyva-Pérez
authors.affiliation: Departamento de Protección de Cultivos, Institute for Sustainable Agriculture (CSIC), Córdoba, Spain.
authors.forename: Antonio
authors.initials: A
authors.lastname: Valverde-Corredor
authors.affiliation: Departamento de Biología Experimental, Universidad de Jaén, Jaén, Spain.
authors.forename: Raquel
authors.initials: R
authors.lastname: Valderrama
authors.affiliation: Departamento de Biología Experimental, Universidad de Jaén, Jaén, Spain.
authors.forename: Jaime
authors.initials: J
authors.lastname: Jiménez-Ruiz
authors.affiliation: Departamento de Arquitectura de Computadores, Universidad de Málaga, Málaga, Spain.
authors.forename: Antonio
authors.initials: A
authors.lastname: Muñoz-Merida
authors.affiliation: Departamento de Arquitectura de Computadores, Universidad de Málaga, Málaga, Spain.
authors.forename: Oswaldo
authors.initials: O
authors.lastname: Trelles
authors.affiliation: Departamento de Biología Experimental, Universidad de Jaén, Jaén, Spain.
authors.forename: Juan Bautista
authors.initials: JB
authors.lastname: Barroso
authors.affiliation: Departamento de Protección de Cultivos, Institute for Sustainable Agriculture (CSIC), Córdoba, Spain.
authors.forename: Jesús
authors.initials: J
authors.lastname: Mercado-Blanco
authors.affiliation: Departamento de Biología Experimental, Universidad de Jaén, Jaén, Spain fjluque@ujaen.es.
authors.forename: Francisco
authors.initials: F
authors.lastname: Luque
mesh_terms: D000064:Acclimatization / Q000502:physiology*; D003080:Cold Temperature; D058639:Cold-Shock Response / Q000502:physiology*; D018506:Gene Expression Regulation, Plant / Q000502:physiology*; D031658:Olea / Q000502:physiology*; D018515:Plant Leaves / Q000378:metabolism*; D059467:Transcriptome / Q000502:physiology*; D015854:Up-Regulation / Q000502:physiology
keywords: Olea europaea; cold-stress; olive; transcriptomics