title: GABPβ2 expression during osteogenic differentiation from human osteoblast-like Saos-2 cells.
abstract: The E26 transformation-specific (ETS) family of transcription factors plays an important role in osteogenic differentiation. Whether GA-binding protein β2 (GABPβ2), a member of the ETS family, is involved in osteogenic differentiation has not been previously reported. In the present study, directed differentiation of human osteoblast-like Saos-2 cells was induced and validated by examining alkaline phosphatase (ALP) activity, presence of mineralized nodule and other phenotypic characteristics of the cells on days 0, 3, 6 and 9, thus establishing their osteogenic potential. Real-time PCR revealed that similarly to the bone-specific transcription factor Runx2, the expression of Gabpb2 in Saos-2 cells also peaked on day 3 and was significantly reduced on days 6 and 9. Immunocytochemical staining showed that changes in the immunoreactivity of GABPβ2 also exhibited a similar trend to that of Runx2. Initially, Runx2 was predominantly localized in the nuclei, while GABPβ2 was relatively diffuse. Both exhibited a significant increase in immunoreactivity on day 3, with presence in both the nuclei and cytoplasm. By day 6, both showed a significant decrease in immunoreactivity and were mainly localized in the nuclei. Therefore, we surmise that GABPβ2, as an ETS family member, may play a regulatory role in early osteoblastic differentiation and potentially act in synergy with Runx2.
authors.forename: Xiaoyuan
authors.initials: X
authors.lastname: Xu
authors.forename: Jianjun
authors.initials: J
authors.lastname: Xiong
authors.forename: Tao
authors.initials: T
authors.lastname: Wang
authors.forename: Meirong
authors.initials: M
authors.lastname: Zheng
authors.forename: Ping
authors.initials: P
authors.lastname: Wu
authors.forename: Xinping
authors.initials: X
authors.lastname: Wang
authors.forename: He
authors.initials: H
authors.lastname: Jiang
authors.forename: Benyi
authors.initials: B
authors.lastname: Yi
authors.forename: Bin
authors.initials: B
authors.lastname: Lang
authors.affiliation: Jiujiang University. liweidong@outlook.com.
authors.forename: Weidong
authors.initials: W
authors.lastname: Li
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D002454:Cell Differentiation / Q000235:genetics; D002478:Cells, Cultured; D051268:GA-Binding Protein Transcription Factor / Q000235:genetics* / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D006801:Humans; D007150:Immunohistochemistry; D010006:Osteoblasts / Q000166:cytology* / Q000201:enzymology; D010012:Osteogenesis / Q000235:genetics*; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D051268:GA-Binding Protein Transcription Factor; D000469:Alkaline Phosphatase