title: A rapid method for preparation of the cerebrospinal fluid proteome.
abstract: The cerebrospinal fluid (CSF) proteome is of great interest for investigation of diseases and conditions involving the CNS. However, the presence of high-abundance proteins (HAPs) can interfere with the detection of low-abundance proteins, potentially hindering the discovery of new biomarkers. Therefore, an assessment of the CSF subproteome composition requires depletion strategies. Existing methods are time consuming, often involving multistep protocols. Here, we present a rapid, accurate, and reproducible method for preparing the CSF proteome, which allows the identification of a high number of proteins. This method involves acetonitrile (ACN) precipitation for depleting HAPs, followed by immediate trypsination. As an example, we demonstrate that this method allows discrimination between multiple sclerosis patients and healthy subjects.
authors.affiliation: Research Department, Stavanger University Hospital, Stavanger, Norway; International Research Institute of Stavanger, IRIS Envrionment, Stavanger, Norway.
authors.forename: Eivind
authors.initials: E
authors.lastname: Larssen
authors.forename: Cato
authors.initials: C
authors.lastname: Brede
authors.forename: Anne Bjørnstad
authors.initials: AB
authors.lastname: Hjelle
authors.forename: Kjell Birger
authors.initials: KB
authors.lastname: Øysaed
authors.forename: Anne Bolette
authors.initials: AB
authors.lastname: Tjensvoll
authors.forename: Roald
authors.initials: R
authors.lastname: Omdal
authors.forename: Peter
authors.initials: P
authors.lastname: Ruoff
mesh_terms: D000097:Acetonitriles / Q000737:chemistry; D002556:Cerebrospinal Fluid Proteins / Q000032:analysis / Q000302:isolation & purification* / Q000378:metabolism; D011232:Chemical Precipitation; D002853:Chromatography, Liquid; D006801:Humans; D020543:Proteome / Q000032:analysis / Q000302:isolation & purification* / Q000378:metabolism; D012598:Sclerosis / Q000134:cerebrospinal fluid*; D053719:Tandem Mass Spectrometry; D014357:Trypsin / Q000378:metabolism
chemical_list: D000097:Acetonitriles; D002556:Cerebrospinal Fluid Proteins; D020543:Proteome; D014357:Trypsin; C032159:acetonitrile
keywords: ACN-precipitation; Cerebrospinal fluid; LC-MS/MS; Sample preparation; Technology; Trypsination