title: Yam (Dioscorea batatas) Root and Bark Extracts Stimulate Osteoblast Mineralization by Increasing Ca and P Accumulation and Alkaline Phosphatase Activity.
abstract: Yam (Dioscorea batatas) is widely consumed as functional food for health promotion mainly in East Asia countries. We assessed whether yam root (tuber) or bark (peel) extracts stimulated the activity of osteoblasts for osteogenesis. MC3T3-E1 cells (mouse osteoblasts) were treated with yam root extracts (water or methanol) (study I) or bark extracts (water or hexane) (study II) within 0~10 μg/mL during the periods of osteoblast proliferation (5~10 day), matrix maturation (11~15 day) and mineralization (16~20 day) as appropriate. In study I, both yam root water and methanol extracts increased cell proliferation as concentration-dependent manner. Cellular collagen synthesis and alkaline phosphatase (ALP) activity, both the indicators of bone matrix protein and inorganic phosphate production for calcification respectively, were also increased by yam root water and methanol extract. Osteoblast calcification as cell matrix Ca and P accumulation was also increased by the addition of yam root extracts. In study II, yam bark extracts (water and hexane) increased osteoblast proliferation and differentiation, as collagen synthesis and ALP activity and osteoblast matrix Ca and P deposition. The study results suggested that both yam root and bark extracts stimulate osteogenic function in osteoblasts by stimulating bone matrix maturation by increasing collagen synthesis, ALP activity, and matrix mineralization.
authors.affiliation: Department of Food and Nutrition, Andong National University, Gyeongbuk 760-749, Korea.
authors.forename: Suji
authors.initials: S
authors.lastname: Kim
authors.affiliation: Department of Food and Nutrition, Andong National University, Gyeongbuk 760-749, Korea.
authors.forename: Mee-Young
authors.initials: MY
authors.lastname: Shin
authors.affiliation: Department of Food and Nutrition, Andong National University, Gyeongbuk 760-749, Korea.
authors.forename: Kun-Ho
authors.initials: KH
authors.lastname: Son
authors.affiliation: Department of Food and Nutrition, Andong National University, Gyeongbuk 760-749, Korea.
authors.forename: Ho-Yong
authors.initials: HY
authors.lastname: Sohn
authors.affiliation: Department of Biological Sciences, Andong National University, Gyeongbuk 760-749, Korea.
authors.forename: Jae-Hwan
authors.initials: JH
authors.lastname: Lim
authors.affiliation: Department of Food Science and Biotechnology, Andong National University, Gyeongbuk 760-749, Korea.
authors.forename: Jong-Hwa
authors.initials: JH
authors.lastname: Lee
authors.affiliation: Department of Food and Nutrition, Andong National University, Gyeongbuk 760-749, Korea.
authors.forename: In-Sook
authors.initials: IS
authors.lastname: Kwun
keywords: alkaline phosphatase; collagen; mineralization; osteogenesis; yam root and bark