title: Effect of additive oxygen gas on cellular response of lung cancer cells induced by atmospheric pressure helium plasma jet.
abstract: The atmospheric pressure helium plasma jet driven by pulsed dc voltage was utilized to treat human lung cancer cells in vitro. The properties of plasma plume were adjusted by the injection type and flow rate of additive oxygen gas in atmospheric pressure helium plasma jet. The plasma characteristics such as plume length, electric current and optical emission spectra (OES) were measured at different flow rates of additive oxygen to helium. The plasma plume length and total current decreased with an increase in the additive oxygen flow rate. The electron excitation temperature estimated by the Boltzmann plot from several excited helium emission lines increased slightly with the additive oxygen flow. The oxygen atom density in the gas phase estimated by actinometry utilizing argon was observed to increase with the additive oxygen flow. The concentration of intracellular reactive oxygen species (ROS) measured by fluorescence assay was found to be not exactly proportional to that of extracellular ROS (measured by OES), but both correlated considerably. It was also observed that the expression levels of p53 and the phospho-p53 were enhanced in the presence of additive oxygen flow compared with those from the pure helium plasma treatment.
authors.affiliation: Department of Physics, Dong-A University, Busan 604-714, Republic of Korea.
authors.forename: Hea Min
authors.initials: HM
authors.lastname: Joh
authors.affiliation: Department of Biological Science, Dong-A University, Busan 604-714, Republic of Korea.
authors.forename: Ji Ye
authors.initials: JY
authors.lastname: Choi
authors.affiliation: Department of Physics, Dong-A University, Busan 604-714, Republic of Korea.
authors.forename: Sun Ja
authors.initials: SJ
authors.lastname: Kim
authors.affiliation: Department of Physics, Dong-A University, Busan 604-714, Republic of Korea.
authors.forename: T H
authors.initials: TH
authors.lastname: Chung
authors.affiliation: Department of Biological Science, Dong-A University, Busan 604-714, Republic of Korea.
authors.forename: Tae-Hong
authors.initials: TH
authors.lastname: Kang
mesh_terms: D001274:Atmospheric Pressure; D045744:Cell Line, Tumor; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006371:Helium / Q000008:administration & dosage*; D006801:Humans; D008175:Lung Neoplasms / Q000378:metabolism / Q000473:pathology* / Q000628:therapy; D010100:Oxygen / Q000008:administration & dosage*; D017382:Reactive Oxygen Species / Q000378:metabolism*; D016159:Tumor Suppressor Protein p53 / Q000096:biosynthesis
chemical_list: D017382:Reactive Oxygen Species; C495901:TP53 protein, human; D016159:Tumor Suppressor Protein p53; D006371:Helium; D010100:Oxygen