title: Anion carrier formation by calix[4]arene-bis-hydroxymethylphosphonic acid in bilayer membranes.
abstract: The action of calix[4]arenes C-91, C-97, C-99, C-107 and C-160 on solvent-containing planar bilayer membranes made of cholesterol and egg phosphatidylcholine (egg PC) or synthetic 18-carbon-tail phospholipid DOPC has been investigated in a voltage-clamp mode. Within the range of calix[4]arenes tested, a steady-state voltage-dependent transmembrane current was achieved only after addition of calix[4]-arene C-99 (calix[4]arene-bis-hydroxymethylphosphonic acid) from the side of the membrane the positive potential was applied to. This current exhibited anion selectivity passing more chloride at negative potentials applied from the side of the membrane to which calix[4]arene C-99 was introduced. The kinetics and temperature-dependence determined for calix[4]arene C-99-mediated ionic transport suggest a carrier mode of facilitated diffusion.
authors.affiliation: Department of Neurochemistry, Palladin Institute of Biochemistry, Leontovich Str., 9, 01601, Kyiv, Ukraine. olegshatursky@biochem.kiev.ua.
authors.forename: Oleg Ya
authors.initials: OY
authors.lastname: Shatursky
authors.forename: Ludmila A
authors.initials: LA
authors.lastname: Kasatkina
authors.forename: Roman V
authors.initials: RV
authors.lastname: Rodik
authors.forename: Sergiy O
authors.initials: SO
authors.lastname: Cherenok
authors.forename: Alexander A
authors.initials: AA
authors.lastname: Shkrabak
authors.forename: Tatiana O
authors.initials: TO
authors.lastname: Veklich
authors.forename: Tatiana A
authors.initials: TA
authors.lastname: Borisova
authors.forename: Sergyi O
authors.initials: SO
authors.lastname: Kosterin
authors.forename: Vitaly I
authors.initials: VI
authors.lastname: Kalchenko
mesh_terms: D000838:Anions / Q000737:chemistry; D047250:Calixarenes / Q000737:chemistry*; D002784:Cholesterol / Q000737:chemistry; D007700:Kinetics; D008051:Lipid Bilayers / Q000737:chemistry*; D008968:Molecular Conformation; D010063:Ovum / Q000737:chemistry; D010713:Phosphatidylcholines / Q000737:chemistry; D013696:Temperature
chemical_list: D000838:Anions; D008051:Lipid Bilayers; D010713:Phosphatidylcholines; C000599266:calix(4)arene-bis-hydroxymethylphosphonic acid; D047250:Calixarenes; D002784:Cholesterol; C017251:1,2-oleoylphosphatidylcholine