title: Recent developments in therapeutic protein expression technologies in plants.
abstract: Infectious diseases and cancers are some of the commonest causes of deaths throughout the world. The previous two decades have witnessed a combined endeavor across various biological sciences to address this issue in novel ways. The advent of recombinant DNA technologies has provided the tools for producing recombinant proteins that can be used as therapeutic agents. A number of expression systems have been developed for the production of pharmaceutical products. Recently, advances have been made using plants as bioreactors to produce therapeutic proteins directed against infectious diseases and cancers. This review highlights the recent progress in therapeutic protein expression in plants (stable and transient), the factors affecting heterologous protein expression, vector systems and recent developments in existing technologies and steps towards the industrial production of plant-made vaccines, antibodies, and biopharmaceuticals.
authors.affiliation: National Key Laboratory of Crop Genetic Improvement, MOA Key Laboratory of Crop Ecophysiology and Farming System in the Middle Reaches of the Yangtze River, College of Plant Science and Technology, Huazhong Agricultural University, No. 1 Shizishan Street, Hongshan District, Wuhan, 430070, Hubei, China.
authors.forename: Shah
authors.initials: S
authors.lastname: Fahad
authors.forename: Faheem Ahmed
authors.initials: FA
authors.lastname: Khan
authors.forename: Nuruliarizki Shinta
authors.initials: NS
authors.lastname: Pandupuspitasari
authors.forename: Muhammad Mahmood
authors.initials: MM
authors.lastname: Ahmed
authors.forename: Yu Cai
authors.initials: YC
authors.lastname: Liao
authors.forename: Muhammad Tahir
authors.initials: MT
authors.lastname: Waheed
authors.forename: Muhammad
authors.initials: M
authors.lastname: Sameeullah
authors.lastname: Darkhshan
authors.forename: Saddam
authors.initials: S
authors.lastname: Hussain
authors.forename: Shah
authors.initials: S
authors.lastname: Saud
authors.forename: Shah
authors.initials: S
authors.lastname: Hassan
authors.forename: Amanullah
authors.initials: A
authors.lastname: Jan
authors.forename: Mohammad Tariq
authors.initials: MT
authors.lastname: Jan
authors.forename: Chao
authors.initials: C
authors.lastname: Wu
authors.forename: Ma Xiao
authors.initials: MX
authors.lastname: Chun
authors.forename: Jianliang
authors.initials: J
authors.lastname: Huang
mesh_terms: D000818:Animals; D000906:Antibodies / Q000235:genetics / Q000378:metabolism; D019149:Bioreactors; D002986:Clinical Trials as Topic; D005822:Genetic Vectors; D006801:Humans; D051379:Mice; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D014612:Vaccines / Q000235:genetics / Q000378:metabolism
chemical_list: D000906:Antibodies; D011994:Recombinant Proteins; D014612:Vaccines