title: A four actin-binding protein signature model for poor prognosis of patients with esophageal squamous cell carcinoma.
abstract: The actin cytoskeleton is a dynamic structure with actin-binding proteins (ABPs) playing an essential role in the regulation of migration, differentiation and signal transduction in all eukaryotic cells. We examined the relationship between altered expression of four ABPs and clinical parameters in esophageal squamous cell carcinoma (ESCC). To this end, we analyzed 152 formalin-fixed and paraffin-embedded esophageal curative resection specimens by immunohistochemistry for tensin, profilin-1, villin-1 and talin. A molecular predictor model, based on the combined expression of the four proteins, was developed to correlate the expression pattern of the four ABPs with clinical factors and prognosis of ESCC. According to the results, weak significance was found for tensin in lymph node metastasis (P=0.033), and profilin-1 in pTNM stage (P=0.031). However, our four-protein model showed strong correlation with the 5-year overall survival rate (P=0.002). Similarly, Kendall's tau-b test also showed the relationship between the collective expression pattern of the four ABPs with lymph node metastasis (P=0.005) and pTNM stage (P=0.001). Our results demonstrate that the collective protein expression pattern of four actin-binding proteins could be a biomarker to estimate the prognosis of ESCC patients.
authors.affiliation: Key Laboratory of Molecular Biology in High Cancer Incidence Coastal Chaoshan Area of Guangdong Higher Education Institutes, Shantou University Medical College Shantou 515041, P. R. China ; Institute of Oncologic Pathology, Shantou University Medical College Shantou 515041, P. R. China.
authors.forename: Zhang-Mei
authors.initials: ZM
authors.lastname: Peng
authors.affiliation: Key Laboratory of Molecular Biology in High Cancer Incidence Coastal Chaoshan Area of Guangdong Higher Education Institutes, Shantou University Medical College Shantou 515041, P. R. China ; Institute of Oncologic Pathology, Shantou University Medical College Shantou 515041, P. R. China.
authors.forename: Wei
authors.initials: W
authors.lastname: Yu
authors.affiliation: Key Laboratory of Molecular Biology in High Cancer Incidence Coastal Chaoshan Area of Guangdong Higher Education Institutes, Shantou University Medical College Shantou 515041, P. R. China ; Institute of Oncologic Pathology, Shantou University Medical College Shantou 515041, P. R. China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Xie
authors.affiliation: Key Laboratory of Molecular Biology in High Cancer Incidence Coastal Chaoshan Area of Guangdong Higher Education Institutes, Shantou University Medical College Shantou 515041, P. R. China ; Institute of Oncologic Pathology, Shantou University Medical College Shantou 515041, P. R. China.
authors.forename: Wei-Hua
authors.initials: WH
authors.lastname: Peng
authors.affiliation: Key Laboratory of Molecular Biology in High Cancer Incidence Coastal Chaoshan Area of Guangdong Higher Education Institutes, Shantou University Medical College Shantou 515041, P. R. China ; Institute of Oncologic Pathology, Shantou University Medical College Shantou 515041, P. R. China.
authors.forename: Hui-Hui
authors.initials: HH
authors.lastname: Cao
authors.affiliation: Department of Pathology, Shantou Central Hospital, Affiliated Shantou Hospital of Sun Yat-Sen University Shantou 515041, P. R. China.
authors.forename: Jin-Hui
authors.initials: JH
authors.lastname: Shen
authors.affiliation: Department of Oncologic Surgery, Shantou Central Hospital, Affiliated Shantou Hospital of Sun Yat-Sen University Shantou 515041, P. R. China.
authors.forename: Zhi-Yong
authors.initials: ZY
authors.lastname: Wu
authors.affiliation: Key Laboratory of Molecular Biology in High Cancer Incidence Coastal Chaoshan Area of Guangdong Higher Education Institutes, Shantou University Medical College Shantou 515041, P. R. China ; Department of Biochemistry and Molecular Biology, Shantou University Medical College Shantou 515041, P. R. China.
authors.forename: En-Min
authors.initials: EM
authors.lastname: Li
authors.affiliation: Key Laboratory of Molecular Biology in High Cancer Incidence Coastal Chaoshan Area of Guangdong Higher Education Institutes, Shantou University Medical College Shantou 515041, P. R. China ; Institute of Oncologic Pathology, Shantou University Medical College Shantou 515041, P. R. China.
authors.forename: Li-Yan
authors.initials: LY
authors.lastname: Xu
mesh_terms: D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000032:analysis*; D002294:Carcinoma, Squamous Cell / Q000737:chemistry* / Q000401:mortality / Q000473:pathology / Q000601:surgery; D004938:Esophageal Neoplasms / Q000737:chemistry* / Q000401:mortality / Q000473:pathology / Q000601:surgery; D000077277:Esophageal Squamous Cell Carcinoma; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D053208:Kaplan-Meier Estimate; D008207:Lymphatic Metastasis; D008297:Male; D008840:Microfilament Proteins / Q000032:analysis*; D008875:Middle Aged; D009367:Neoplasm Staging; D011237:Predictive Value of Tests; D051304:Profilins / Q000032:analysis*; D016016:Proportional Hazards Models; D012189:Retrospective Studies; D016608:Talin / Q000032:analysis*; D000070876:Tensins; D013997:Time Factors; D046888:Tissue Array Analysis
chemical_list: D014408:Biomarkers, Tumor; D008840:Microfilament Proteins; C495738:PFN1 protein, human; D051304:Profilins; D016608:Talin; D000070876:Tensins; C569754:VIL1 protein, human
keywords: Actin-binding protein; a molecular prognostic model; esophageal squamous cell carcinoma; prognosis