title: Extraction and quantification of phenolic acids and flavonols from Eugenia pyriformis using different solvents.
abstract: The recovery of phenolic compounds of Eugenia pyriformis using different solvents was investigated in this study. The compounds were identified and quantified by reverse-phase high-performance liquid chromatography coupled with ultraviolet-visible diode-array detector (RP-HPLC-DAD/UV-vis). Absolute methanol was the most effective extraction agent of phenolic acids and flavonols (588.31 mg/Kg) from Eugenia pyriformis, although similar results (p ≤ 0.05) were observed using methanol/water (1:1 ratio). Our results clearly showed that higher contents of phenolic compounds were not obtained either with the most or the least polar solvents used. Several phenolic compounds were identified in the samples whereas gallic acid and quercetin were the major compounds recovered.
authors.affiliation: Programa de Pós-Graduação em Tecnologia de Alimentos (PPGTA), Universidade Tecnológica Federal do Paraná (UTFPR), Campus Campo Mourão, PR Brasil.
authors.forename: Charles Windson Isidoro
authors.initials: CW
authors.lastname: Haminiuk
authors.affiliation: Programa de Pós-Graduação em Tecnologia de Alimentos (PPGTA), Universidade Tecnológica Federal do Paraná (UTFPR), Campus Campo Mourão, PR Brasil.
authors.forename: Manuel Salvador Vicente
authors.initials: MS
authors.lastname: Plata-Oviedo
authors.affiliation: Programa de Pós-Graduação em Tecnologia de Alimentos (PPGTA), Universidade Tecnológica Federal do Paraná (UTFPR), Campus Campo Mourão, PR Brasil.
authors.forename: Gisely
authors.initials: G
authors.lastname: de Mattos
authors.affiliation: Programa de Pós-Graduação de Tecnologia de Processos Químicos e Bioquímicos (PPGTP), Universidade Tecnológica Federal do Paraná (UTFPR), Campus Pato Branco, PR Brasil.
authors.forename: Solange Teresinha
authors.initials: ST
authors.lastname: Carpes
authors.affiliation: Universidade Estadual Paulista (UNESP), Campus Assis, Assis, SP Brasil.
authors.forename: Ivanise Guilherme
authors.initials: IG
authors.lastname: Branco
keywords: Eugenia pyriformis; Extraction; Flavonols; HPLC; Phenolic acids; Solvents