title: Gluconic acid production from sucrose in an airlift reactor using a multi-enzyme system.
abstract: Sucrose from sugarcane is produced in abundance in Brazil, which provides an opportunity to manufacture other high-value products. Gluconic acid (GA) can be produced by multi-enzyme conversion of sucrose using the enzymes invertase, glucose oxidase, and catalase. In this process, one of the byproducts is fructose, which has many commercial applications. This work concerns the batch mode production of GA in an airlift reactor fed with sucrose as substrate. Evaluation was made of the influence of temperature and pH, as well as the thermal stability of the enzymes. Operational conditions of 40 °C and pH 6.0 were selected, based on the enzymatic activity profiles and the thermal stabilities. Under these conditions, the experimental data could be accurately described by kinetic models. The maximum yield of GA was achieved within 3.8 h, with total conversion of sucrose and glucose and a volumetric productivity of around 7.0 g L(-1) h(-1).
authors.affiliation: Postgraduate Program in Chemical Engineering of the Federal University of São Carlos (PPGEQ/UFSCar), Rodovia Washington Luiz, km 235, São Carlos, SP, 13565-905, Brazil.
authors.forename: Agnes Cristina Oliveira
authors.initials: AC
authors.lastname: Mafra
authors.forename: Felipe Fernando
authors.initials: FF
authors.lastname: Furlan
authors.forename: Alberto Colli
authors.initials: AC
authors.lastname: Badino
authors.forename: Paulo Waldir
authors.initials: PW
authors.lastname: Tardioli
mesh_terms: D019149:Bioreactors; D002374:Catalase / Q000737:chemistry; D002845:Chromatography; D004800:Enzymes, Immobilized / Q000737:chemistry; D005632:Fructose / Q000737:chemistry; D005942:Gluconates / Q000737:chemistry*; D005947:Glucose / Q000737:chemistry; D005949:Glucose Oxidase / Q000737:chemistry; D006861:Hydrogen Peroxide / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D007218:Industrial Microbiology; D013395:Sucrose / Q000737:chemistry*; D013696:Temperature; D043324:beta-Fructofuranosidase / Q000737:chemistry
chemical_list: D004800:Enzymes, Immobilized; D005942:Gluconates; D005632:Fructose; D013395:Sucrose; D006861:Hydrogen Peroxide; D005949:Glucose Oxidase; D002374:Catalase; D043324:beta-Fructofuranosidase; D005947:Glucose; C030691:gluconic acid