title: Interaction among the vacuole, the mitochondria, and the oxidative stress response is governed by the transient receptor potential channel in Candida albicans.
abstract: Candida albicans is one of the most important opportunistic pathogens, causing both mucosal candidiasis and life-threatening systemic infections. To survive in the host immune defense system, this pathogen uses an elaborate signaling network to recognize and respond to oxidative stress, which is essential for its pathogenicity. However, the exact mechanisms that this fungus employs to integrate the oxidative stress response (OSR) with functions of various organelles remain uncharacterized. Our previous work implicated a connection between the calcium signaling system and the OSR. In this study, we find that the vacuolar transient receptor potential (TRP) channel Yvc1, one of the calcium signaling members, plays a critical role in cell tolerance to oxidative stress. We further provide evidence that this channel is required not only for activation of Cap1-related transcription of OSR genes but also for maintaining the stability of both the mitochondria and the vacuole in a potassium- and calcium-dependent manner. Element assays reveal that this TRP channel affects calcium influx and potassium transport from the vacuole to the mitochondria. Therefore, the TRP channel governs the novel interaction among the OSR, the vacuole, and the mitochondria by mediating ion transport in this pathogen under oxidative stress.
authors.affiliation: Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, College of Life Science, Nankai University, Tianjin 300071, People's Republic of China.
authors.forename: Qilin
authors.initials: Q
authors.lastname: Yu
authors.affiliation: Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, College of Life Science, Nankai University, Tianjin 300071, People's Republic of China.
authors.forename: Bing
authors.initials: B
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, College of Life Science, Nankai University, Tianjin 300071, People's Republic of China.
authors.forename: Baopeng
authors.initials: B
authors.lastname: Yang
authors.affiliation: Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, College of Life Science, Nankai University, Tianjin 300071, People's Republic of China.
authors.forename: Jiatong
authors.initials: J
authors.lastname: Chen
authors.affiliation: Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, College of Life Science, Nankai University, Tianjin 300071, People's Republic of China; Agro-Environmental Protection Institute, Ministry of Agriculture, Tianjin 300191, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Wang
authors.affiliation: Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, College of Life Science, Nankai University, Tianjin 300071, People's Republic of China.
authors.forename: Chang
authors.initials: C
authors.lastname: Jia
authors.affiliation: Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, College of Life Science, Nankai University, Tianjin 300071, People's Republic of China.
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Ding
authors.affiliation: Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, College of Life Science, Nankai University, Tianjin 300071, People's Republic of China.
authors.forename: Ning
authors.initials: N
authors.lastname: Xu
authors.affiliation: Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, College of Life Science, Nankai University, Tianjin 300071, People's Republic of China.
authors.forename: Yijie
authors.initials: Y
authors.lastname: Dong
authors.affiliation: Tianjin Traditional Chinese Medicine University, Tianjin 300193, People's Republic of China.
authors.forename: Biao
authors.initials: B
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, College of Life Science, Nankai University, Tianjin 300071, People's Republic of China.
authors.forename: Laijun
authors.initials: L
authors.lastname: Xing
authors.affiliation: Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, College of Life Science, Nankai University, Tianjin 300071, People's Republic of China. Electronic address: nklimingchun@163.com.
authors.forename: Mingchun
authors.initials: M
authors.lastname: Li
mesh_terms: D000818:Animals; D017209:Apoptosis; D001692:Biological Transport; D020013:Calcium Signaling; D002176:Candida albicans / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D002374:Catalase / Q000378:metabolism; D002478:Cells, Cultured; D005260:Female; D005656:Fungal Proteins / Q000502:physiology*; D015966:Gene Expression Regulation, Fungal; D006861:Hydrogen Peroxide / Q000494:pharmacology; D008264:Macrophages / Q000382:microbiology; D053078:Membrane Potential, Mitochondrial; D008813:Mice, Inbred ICR; D008928:Mitochondria / Q000378:metabolism; D016877:Oxidants / Q000494:pharmacology; D018384:Oxidative Stress; D013482:Superoxide Dismutase / Q000378:metabolism; D050051:Transient Receptor Potential Channels / Q000502:physiology*; D014617:Vacuoles / Q000502:physiology*
chemical_list: D005656:Fungal Proteins; D016877:Oxidants; D050051:Transient Receptor Potential Channels; D006861:Hydrogen Peroxide; D002374:Catalase; D013482:Superoxide Dismutase
keywords: Calcium signaling system; Candida albicans; Mitochondrion; Oxidative stress response; Transient receptor potential channel; Vacuole