title: Inhibition of biofilm formation in Bacillus subtilis by new halogenated furanones.
abstract: Gram-positive bacteria can cause various infections including hospital-acquired infections. While in the biofilm, the resistance of bacteria to both antibiotics and the human immune system is increased causing difficulties in the treatment. Bacillus subtilis, a non-pathogenic Gram-positive bacterium, is widely used as a model organism for studying biofilm formation. Here we investigated the effect of novel synthesized chloro- and bromo-containing 2(5H)-furanones on biofilm formation by B. subtilis. Mucobromic acid (3,4-dibromo-5-hydroxy-2(5H)-furanone) and the two derivatives of mucochloric acid (3,4-dichloro-5-hydroxy-2(5H)-furanone)-F8 and F12-were found to inhibit the growth and to efficiently prevent biofilm formation by B. subtilis. Along with the low production of polysaccharide matrix and repression of the eps operon, strong repression of biofilm-related yqxM also occurred in the presence of furanones. Therefore, our data confirm that furanones affect significantly the regulatory pathway(s) leading to biofilm formation. We propose that the global regulator, Spo0A, is one of the potential putative cellular targets for these compounds.
authors.affiliation: Department of Genetics, Kazan (Volga Region) Federal University, Kazan, Russia.
authors.forename: Airat R
authors.initials: AR
authors.lastname: Kayumov
authors.affiliation: Department of Genetics, Kazan (Volga Region) Federal University, Kazan, Russia.
authors.forename: Elvina N
authors.initials: EN
authors.lastname: Khakimullina
authors.affiliation: Department of Genetics, Kazan (Volga Region) Federal University, Kazan, Russia.
authors.forename: Irshad S
authors.initials: IS
authors.lastname: Sharafutdinov
authors.affiliation: Department of Genetics, Kazan (Volga Region) Federal University, Kazan, Russia.
authors.forename: Elena Y
authors.initials: EY
authors.lastname: Trizna
authors.affiliation: A.M. Butlerov Institute of Chemistry, Kazan (Volga Region) Federal University, Kazan, Russia.
authors.forename: Lilia Z
authors.initials: LZ
authors.lastname: Latypova
authors.affiliation: A.M. Butlerov Institute of Chemistry, Kazan (Volga Region) Federal University, Kazan, Russia.
authors.forename: Hoang
authors.initials: H
authors.lastname: Thi Lien
authors.affiliation: Department of Genetics, Kazan (Volga Region) Federal University, Kazan, Russia.
authors.forename: Anna B
authors.initials: AB
authors.lastname: Margulis
authors.affiliation: Biomedical Engineering Research Center, St Petersburg State Electrotechnical University, St Petersburg, Russia.
authors.forename: Mikhail I
authors.initials: MI
authors.lastname: Bogachev
authors.affiliation: A.M. Butlerov Institute of Chemistry, Kazan (Volga Region) Federal University, Kazan, Russia.
authors.forename: Almira R
authors.initials: AR
authors.lastname: Kurbangalieva
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology*; D001412:Bacillus subtilis / Q000502:physiology*; D018441:Biofilms / Q000187:drug effects* / Q000254:growth & development*; D005663:Furans / Q000737:chemistry / Q000494:pharmacology*; D006843:Hydrocarbons, Chlorinated / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests; D015394:Molecular Structure
chemical_list: C000600011:3,4-dichloro-5-((1,3-dichloropropan-2-yloxy))-2(5H)-furanone; C000600012:3-chloro-5-hydroxy-4-((4-methylphenylsulfanyl))-2(5H)-furanone; D000900:Anti-Bacterial Agents; D005663:Furans; D006843:Hydrocarbons, Chlorinated; C014116:mucobromic acid