title: Salmonella enterica serovar Enteritidis and  Listeria monocytogenes in mango (Mangifera  indica L.) pulp: growth, survival and  cross-contamination.
abstract: BACKGROUND
  This study examined the ability of Salmonella enterica serovar Enteritidis and Listeria monocytogenes to grow or survive in mango pulp stored at -20°C, 4°C, 10°C and 25°C, as well as to cross-contaminate mangoes by means of a knife contaminated with different levels of these pathogens.
  
  
  RESULTS
  At 25°C lag phase durations of 19 h and 7.2 h and generation times of 0.66 and 1.44 were obtained, respectively, for S. Enteritidis and L. monocytogenes. At 10°C only the growth of L. monocytogenes was observed. At 4°C both bacteria survived for 8 days. At -20°C S. Enteritidis was able to survive for 5 months while L. monocytogenes survived for 8 months. Cross-contamination was observed for knives contaminated with 10⁶, 10⁵ and 10⁴ CFU mL⁻¹ of S. Enteritidis and 10⁶ and 10⁵ CFU mL⁻¹ of L. monocytogenes.
  
  
  CONCLUSIONS
  Both microorganisms can grow well in mango pulp at 25°C, thus lower temperatures for the maintenance of the pulps are crucial to avoid growth of these microorganisms. A refrigeration temperature of 10°C will avoid only the growth of S. Enteritidis. Thus good handling practices should be rigidly enforced to avoid any contamination as even at refrigeration and freezing temperatures survival of these pathogens may occur.
authors.forename: Ana L
authors.initials: AL
authors.lastname: Penteado
authors.forename: M Fernanda P M
authors.initials: MF
authors.lastname: de Castro
authors.forename: Ana C B
authors.initials: AC
authors.lastname: Rezende
mesh_terms: D001938:Brazil; D003080:Cold Temperature; D015169:Colony Count, Microbial; D003297:Cooking and Eating Utensils; D004866:Equipment Contamination; D005511:Food Handling; D061353:Food Storage; D005628:Frozen Foods / Q000382:microbiology; D005638:Fruit / Q000382:microbiology*; D008089:Listeria monocytogenes / Q000254:growth & development* / Q000302:isolation & purification; D031022:Mangifera / Q000382:microbiology*; D050296:Microbial Viability; D008954:Models, Biological; D012034:Refrigeration; D012477:Salmonella enteritidis / Q000254:growth & development* / Q000302:isolation & purification; D013997:Time Factors