title: [The effect of hypoxia on proliferation and osteogenic differentiation of periodontal ligament cells].
abstract: OBJECTIVE
  To investigate the effect of hypoxia on proliferation and osteogenic differentiation of periodontal ligament cells (PDLCs) in vitro.
  
  
  METHODS
  Human PDLCs were isolated and characterized. The proliferation rate of PDLCs under different concentration of CoCl(2) were tested by MTT assay. The PDLCs' osteogenic differentiation were investigated using real-time PCR and Western blot. The date was statistically analyzed with SPSS13.0 software package.
  
  
  RESULTS
  Immunocytochemical staining verified that the isolated cells were PDLCs. The proliferation of PDLCs and the expression of alkaline phosphatase (ALP), RUNX2, collagen I were significantly decreased in a dose-dependent manner by 200 μmol/L CoCl(2) and 400 μmol/L CoCl(2).
  
  
  CONCLUSIONS
  Hypoxia inhibits proliferation and osteogenic differentiation of human PDLCs.
authors.affiliation: Department of Periodontology, Ninth People's Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine. Shanghai 200011;China.E-mail:dongjiachensky@163.com.
authors.forename: Jia-chen
authors.initials: JC
authors.lastname: Dong
authors.forename: Zhong-chen
authors.initials: ZC
authors.lastname: Song
authors.forename: Rong
authors.initials: R
authors.lastname: Shu
authors.forename: Song
authors.initials: S
authors.lastname: Li
authors.forename: Zhi-kai
authors.initials: ZK
authors.lastname: Lin
authors.forename: Xiu-li
authors.initials: XL
authors.lastname: Zhang
mesh_terms: D000469:Alkaline Phosphatase; D002454:Cell Differentiation; D006801:Humans; D000860:Hypoxia; D010012:Osteogenesis; D010513:Periodontal Ligament
chemical_list: D000469:Alkaline Phosphatase