title: Identification of microRNAs in human plasma.
abstract: In recent years, microRNAs (miRNA) have been demonstrated to be present in body fluids and may therefore serve as diagnostic markers for diseases. By characterizing miRNA profiles in plasma, a miRNA signature may potentially be developed as a diagnostic and risk assessment tool for particular (patho)physiological states. This chapter describes the isolation, purification, identification, and sequencing of human plasma miRNAs.
authors.affiliation: Department of Internal Medicine, Erasmus MC, Room Ee585b, 3000 DR, 1738, Rotterdam, The Netherlands, b.vandereerden@erasmusmc.nl.
authors.forename: Bram C
authors.initials: BC
authors.lastname: van der Eerden
authors.forename: Rodrigo D
authors.initials: RD
authors.lastname: Alves
authors.forename: Christel E
authors.initials: CE
authors.lastname: Kockx
authors.forename: Zeliha
authors.initials: Z
authors.lastname: Ozgur
authors.forename: Marijke
authors.initials: M
authors.lastname: Schreuders-Koedam
authors.forename: Jeroen
authors.initials: J
authors.lastname: van de Peppel
authors.forename: Wilfred F
authors.initials: WF
authors.lastname: van Ijcken
authors.forename: Johannes P
authors.initials: JP
authors.lastname: van Leeuwen
mesh_terms: D006801:Humans; D035683:MicroRNAs / Q000097:blood / Q000235:genetics / Q000302:isolation & purification; D017423:Sequence Analysis, RNA / Q000379:methods*
chemical_list: D035683:MicroRNAs