title: Biomechanical analysis of optic nerve injury treated by compound light granules and ciliary neurotrophic factor.
abstract: In this study, rabbit models of optic nerve injury were reproduced by the clamp method. After modeling, rabbit models were given one injection of 50 ng recombinant human ciliary neurotrophic factor into the vitreous body and/or intragastric injection of 4 g/kg compound light granules containing Radix Angelicae Sinensis and Raidix Paeoniae Alba at 4 days after modeling, once per day for 30 consecutive days. After administration, the animals were sacrificed and the intraorbital optic nerve was harvested. Hematoxylin-eosin staining revealed that the injured optic nerve was thinner and optic nerve fibers were irregular. After treatment with recombinant human ciliary neurotrophic factor, the arrangement of optic nerve fibers was disordered but they were not markedly thinner. After treatment with compound light granules, the arrangement of optic nerve fibers was slightly disordered and their structure was intact. After combined treatment with recombinant human ciliary neurotrophic factor and compound light granules, the arrangement of optic nerve fibers was slightly disordered and the degree of injury was less than after either treatment alone. Results of tensile mechanical testing of the optic nerve showed that the tensile elastic limit strain, elastic limit stress, maximum stress and maximum strain of the injured optic nerve were significantly lower than the normal optic nerve. After treatment with recombinant human ciliary neurotrophic factor and/or compound light granules, the tensile elastic limit strain, elastic limit stress, maximum stress and maximum strain of the injured optic nerve were significantly increased, especially after the combined treatment. These experimental findings indicate that compound light granules and ciliary neurotrophic factor can alleviate optic nerve injury at the histological and biochemical levels, and the combined treatment is more effective than either treatment alone.
authors.affiliation: Department of Ophthalmology, the Second Hospital of Jilin University, Changchun 130022, Jilin Province, China ; Department of Ophthalmology, China-Japan Union Hospital of Jilin University, Changchun 130033, Jilin Province, China.
authors.forename: Yuying
authors.initials: Y
authors.lastname: Jiang
authors.affiliation: Department of Ophthalmology, the Second Hospital of Jilin University, Changchun 130022, Jilin Province, China.
authors.forename: Haitao
authors.initials: H
authors.lastname: Xu
authors.affiliation: Department of Ophthalmology, China-Japan Union Hospital of Jilin University, Changchun 130033, Jilin Province, China.
authors.forename: Jingxiang
authors.initials: J
authors.lastname: Liu
authors.affiliation: Department of Engineering Mechanics, Nanling Campus of Jilin University, Changchun 130022, Jilin Province, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Li
authors.affiliation: Department of Ophthalmology, the Second Hospital of Jilin University, Changchun 130022, Jilin Province, China.
authors.forename: Yazhen
authors.initials: Y
authors.lastname: Wu
keywords: biomechanics; ciliary neurotrophic factor; compound light granules; mechanical characteristics; neural regeneration; optic nerve injury; retinal ganglial cells; strain; stress; tissue morphology; traditional Chinese medicine