title: A search for Clostridium difficile ribotypes 027 and 078 in Brazil.
abstract: Toxigenic strains of Clostridium difficile may be disseminating. Here we prospectively screened patients with nosocomial diarrhoea in two hospitals in Brazil. To identify C. difficile polymerase chain reaction ribotypes 027/078 strains, we used high resolution melting and multiplex polymerase chain reaction. Among 116 screened patients, 11 were positive for C. difficile. The polymerase chain reaction ribotypes 027/078 strains were not identified in this study.
authors.affiliation: PPG Patologia, Universidade Federal de Ciências da Saúde de Porto Alegre, Porto Alegre, RS, Brazil.
authors.forename: Alexandre de Almeida
authors.initials: Ade A
authors.lastname: Monteiro
authors.affiliation: PPG Patologia, Universidade Federal de Ciências da Saúde de Porto Alegre, Porto Alegre, RS, Brazil; Santa Casa de Misericórdia de Porto Alegre, Porto Alegre, RS, Brazil.
authors.forename: Renata Neto
authors.initials: RN
authors.lastname: Pires
authors.affiliation: Statens Serum Institut, Copenhagen, Denmark.
authors.forename: Søren
authors.initials: S
authors.lastname: Persson
authors.affiliation: Grupo Hospitalar Conceição, Porto Alegre, RS, Brazil.
authors.forename: Edison Moraes
authors.initials: EM
authors.lastname: Rodrigues Filho
authors.affiliation: PPG Patologia, Universidade Federal de Ciências da Saúde de Porto Alegre, Porto Alegre, RS, Brazil; Santa Casa de Misericórdia de Porto Alegre, Porto Alegre, RS, Brazil. Electronic address: acpasqualotto@hotmail.com.
authors.forename: Alessandro Comarú
authors.initials: AC
authors.lastname: Pasqualotto
mesh_terms: D001938:Brazil; D016360:Clostridioides difficile / Q000235:genetics*; D003015:Clostridium Infections / Q000382:microbiology*; D003428:Cross Infection / Q000382:microbiology*; D003967:Diarrhea / Q000382:microbiology*; D005243:Feces / Q000382:microbiology; D006801:Humans; D016133:Polymerase Chain Reaction; D011446:Prospective Studies; D021521:Ribotyping
keywords: Clostridium difficile; HRM; Multiplex-PCR; Toxin gene profiling