title: Investigation of membrane protein-protein interactions using correlative FRET-PLA.
abstract: Fluorescence resonance energy transfer (FRET) analysis and the recently developed proximity ligation assay (PLA) are widely used to study protein-protein interactions in situ. We have developed correlative FRET-PLA to monitor interactions between membrane proteins that frequently cause problems in confirmatory co-immunoprecipitation assays. Correlative FRET-PLA is particularly aimed at delivering robust and reliable results and is useful for investigating protein-protein interactions.
authors.affiliation: Robert Koch Institute, Berlin, Germany; Freie Universität Berlin, Berlin, Germany.
authors.forename: Daniel
authors.initials: D
authors.lastname: Ivanusic
authors.affiliation: Robert Koch Institute, Berlin, Germany.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Eschricht
authors.affiliation: Robert Koch Institute, Berlin, Germany.
authors.forename: Joachim
authors.initials: J
authors.lastname: Denner
mesh_terms: D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D057809:HEK293 Cells; D006801:Humans; D008565:Membrane Proteins / Q000378:metabolism*; D038761:Photobleaching; D016133:Polymerase Chain Reaction / Q000379:methods*; D025941:Protein Interaction Mapping / Q000379:methods*; D015203:Reproducibility of Results
chemical_list: D008565:Membrane Proteins
keywords: CD63; FRET; HIV-1; PLA; protein-protein interaction