title: Engineering short peptide sequences for uranyl binding.
abstract: Peptides are interesting tools to rationalize uranyl-protein interactions, which are relevant to uranium toxicity in vivo. Structured cyclic peptide scaffolds were chosen as promising candidates to coordinate uranyl thanks to four amino acid side chains pre-oriented towards the dioxo cation equatorial plane. The binding of uranyl by a series of decapeptides has been investigated with complementary analytical and spectroscopic methods to determine the key parameters for the formation of stable uranyl-peptide complexes. The molar ellipticity of the uranyl complex at 195 nm is directly correlated to its stability, which demonstrates that the β-sheet structure is optimal for high stability in the peptide series. Cyclodecapeptides with four glutamate residues exhibit the highest affinities for uranyl with log KC =8.0-8.4 and, therefore, appear as good starting points for the design of high-affinity uranyl-chelating peptides.
authors.affiliation: Univ. Grenoble Alpes, INAC, SCIB, 38000 Grenoble (France); CEA, INAC, SCIB, 38054 Grenoble (France).
authors.forename: Colette
authors.initials: C
authors.lastname: Lebrun
authors.forename: Matthieu
authors.initials: M
authors.lastname: Starck
authors.forename: Vicky
authors.initials: V
authors.lastname: Gathu
authors.forename: Yves
authors.initials: Y
authors.lastname: Chenavier
authors.forename: Pascale
authors.initials: P
authors.lastname: Delangle
mesh_terms: D000595:Amino Acid Sequence; D001665:Binding Sites; D002264:Carboxylic Acids / Q000737:chemistry; D009842:Oligopeptides / Q000737:chemistry* / Q000378:metabolism*; D010456:Peptides, Cyclic / Q000737:chemistry / Q000378:metabolism; D014501:Uranium / Q000378:metabolism*
chemical_list: D002264:Carboxylic Acids; D009842:Oligopeptides; D010456:Peptides, Cyclic; D014501:Uranium
keywords: bioinorganic chemistry; carboxylate ligands; fluorescence; peptides; uranium