title: Immunogenicity of a fusion protein comprising coli surface antigen 3 and labile B subunit of enterotoxigenic Escherichia coli.
abstract: Enterotoxigenic Escherichia coli (ETEC) strains are the major causes of diarrheal disease in humans and animals. Colonization factors and enterotoxins are the major virulence factors in ETEC pathogenesis. For the broad-spectrum protection against ETEC, one could focus on colonization factors and non-toxic heat labile as a vaccine candidate.
  
  
  
  A fusion protein is composed of a major fimbrial subunit of coli surface antigen 3, and the heat-labile B subunit (LTB) was constructed as a chimeric immunogen. For optimum level expression of protein, the gene was synthesized with codon bias of E. coli. Also, recombinant protein was expressed in E. coli BL21DE3. ELISA and Western tests were carried out for determination of antigen and specificity of antibody raised against recombinant protein in animals. The anti-toxicity and anti-adherence properties of the immune sera against ETEC were also evaluated.
  
  
  
  Immunological analyses showed the production of high titer of specific antibody in immunized mice. The built-in LTB retains native toxin properties which were approved by GM1 binding assay. Pre-treatment of the ETEC cells with anti-sera significantly decreased their adhesion to Caco-2 cells.
  
  
  
  The results indicated the efficacy of the recombinant chimeric protein as an effective immunogen inducing strong humoral response. The designated chimer would be an interesting prototype for a vaccine and worthy of further investigation.
authors.affiliation: Dept. of Biology, Shahed University, Tehran, Iran.
authors.forename: Masoome
authors.initials: M
authors.lastname: Alerasol
authors.affiliation: Dept. of Biology, Shahed University, Tehran, Iran.
authors.forename: Seyed Latif
authors.initials: SL
authors.lastname: Mousavi Gargari
authors.affiliation: Depent of Biology, Imam Hussein University, Tehran, Iran.
authors.forename: Shahram
authors.initials: S
authors.lastname: Nazarian
authors.affiliation: Dept. of Biology, Shahed University, Tehran, Iran.
authors.forename: Samane
authors.initials: S
authors.lastname: Bagheri
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis; D000942:Antigens, Bacterial / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D001427:Bacterial Toxins / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D018938:Caco-2 Cells; D054307:Enterotoxigenic Escherichia coli / Q000235:genetics / Q000276:immunology* / Q000472:pathogenicity; D004768:Enterotoxins / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D004927:Escherichia coli Infections / Q000276:immunology / Q000517:prevention & control; D029968:Escherichia coli Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D022361:Escherichia coli Vaccines / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D005260:Female; D036781:Fimbriae Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D005798:Genes, Bacterial; D005813:Genes, Synthetic; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D008958:Models, Molecular; D011487:Protein Conformation; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D014614:Vaccines, Synthetic / Q000737:chemistry / Q000235:genetics / Q000276:immunology
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001427:Bacterial Toxins; D004768:Enterotoxins; D029968:Escherichia coli Proteins; D022361:Escherichia coli Vaccines; D011993:Recombinant Fusion Proteins; D014614:Vaccines, Synthetic; C081391:nfaE protein, E coli; D036781:Fimbriae Proteins; C027538:heat-labile enterotoxin, E coli
keywords: Recombinant vaccine; Enterotoxigenic Escherichia coli; cstH; eltB