title: Ultrasensitive UPLC-MS-MS method for the quantitation of etheno-DNA adducts in human urine.
abstract: Etheno-DNA adducts are generated from the metabolism of exogenous carcinogens and endogenous lipid peroxidation. We and others have previously reported that 1,N6-ethenodeoxyadenosine (εdA) and 3,N4-ethenodeoxycytidine (εdC) are present in human urine and can be utilized as biomarkers of oxidative stress. In this study, we report a new ultrasensitive UPLC-ESI-MS/MS method for the analysis of εdA and edC in human urine, capable of detecting 0.5 fmol εdA and 0.3 fmol εdC in 1.0 mL of human urine, respectively. For validation of the method, 20 human urine samples were analyzed, and the results revealed that the mean levels of εdA and εdC (SD) fmol/µmol creatinine are 5.82 ± 2.11 (range 3.0-9.5) for εdA and 791.4 ± 328.8 (range 116.7-1264.9) for εdC in occupational benzene-exposed workers and 2.10 ± 1.32 (range 0.6-4.7) for εdA and 161.8 ± 200.9 (range 1.8-557.5) for εdC in non-benzene-exposed workers, respectively. The ultrasensitive detection method is thus suitable for applications in human biomonitoring and molecular epidemiology studies.
authors.affiliation: Key Laboratory of Chemical Safety and Health, Chinese Center for Disease Control and Prevention, Beijing 100050, China. zsszz@126.com.
authors.forename: Shiwei
authors.initials: S
authors.lastname: Cui
authors.affiliation: Key Laboratory of Chemical Safety and Health, Chinese Center for Disease Control and Prevention, Beijing 100050, China. zsszz@126.com.
authors.forename: Haibin
authors.initials: H
authors.lastname: Li
authors.affiliation: Key Laboratory of Chemical Safety and Health, Chinese Center for Disease Control and Prevention, Beijing 100050, China. wangsj218@sina.com.
authors.forename: Shaojia
authors.initials: S
authors.lastname: Wang
authors.affiliation: Key Laboratory of Chemical Safety and Health, Chinese Center for Disease Control and Prevention, Beijing 100050, China. yayafairyjiang@163.com.
authors.forename: Xiao
authors.initials: X
authors.lastname: Jiang
authors.affiliation: Department of Chemistry, Zhengzhou University, Zhengzhou 450001, Henan province, China. zsszz@126.com.
authors.forename: Shusheng
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Henan Center for Disease Control and Prevention, Zhengzhou 450016, Henan province, China. zhangrj@hncdc.com.cn.
authors.forename: Rongjie
authors.initials: R
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Chemical Safety and Health, Chinese Center for Disease Control and Prevention, Beijing 100050, China. sunxin@chinacdc.cn.
authors.forename: Xin
authors.initials: X
authors.lastname: Sun
mesh_terms: D015415:Biomarkers / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D018736:DNA Adducts / Q000032:analysis / Q000652:urine*; D003839:Deoxyadenosines / Q000737:chemistry / Q000652:urine; D003841:Deoxycytidine / Q000031:analogs & derivatives / Q000737:chemistry / Q000652:urine; D004781:Environmental Exposure / Q000032:analysis* / Q000706:statistics & numerical data; D004784:Environmental Monitoring / Q000379:methods; D006801:Humans; D015227:Lipid Peroxidation / Q000502:physiology; D018384:Oxidative Stress / Q000502:physiology*; D053719:Tandem Mass Spectrometry / Q000379:methods
chemical_list: D015415:Biomarkers; D018736:DNA Adducts; D003839:Deoxyadenosines; D003841:Deoxycytidine