title: NMR analysis of budding yeast metabolomics: a rapid method for sample preparation.
abstract: Here we propose the optimization of a rapid and reproducible protocol for intracellular metabolite extraction from yeast cells and their metabolic profiling by (1)H-NMR spectroscopy. The protocol reliability has been validated through comparison between the metabolome of cells in different phases of growth or with different genetic backgrounds.
authors.affiliation: Department of Biotechnology and Biosciences, University of Milano-Bicocca, Milan, Italy. cristina.airoldi@unimib.it paola.coccetti@unimib.it.
authors.forename: C
authors.initials: C
authors.lastname: Airoldi
authors.forename: F
authors.initials: F
authors.lastname: Tripodi
authors.forename: C
authors.initials: C
authors.lastname: Guzzi
authors.forename: R
authors.initials: R
authors.lastname: Nicastro
authors.forename: P
authors.initials: P
authors.lastname: Coccetti
mesh_terms: D055442:Metabolome; D055432:Metabolomics / Q000379:methods*; D066244:Proton Magnetic Resonance Spectroscopy / Q000379:methods*; D004718:Saccharomycetales / Q000166:cytology / Q000254:growth & development / Q000378:metabolism*