title: [Inhibition of MCF-7/ADR cells by DOX-loaded pluronic-attached PAMAM dendrimer conjugate].
abstract: Pluronic modified polyamidoamine (PAMAM) conjugate (PF127-PAMAM) was prepared and the inhibiting effect of MDR against MCF-7/ADR was investigated with doxorubicin (DOX) as model drug. 1H NMR and FTIR spectra showed that the conjugate was synthesized successfully. Element analysis accurately measured that 27.63% amino of per PAMAM was modified by pluronic (PAMAM : PF127, 1 : 35.37 mole ratio). PF127-PAMAM showed an increased size and a reduced zeta potential compared to PAMAM. PF127-PAMAM had lower hemolytic toxicity and cytotoxicity due to the reduced zeta potential and the protection of PF127. Each PF127-PAMAM molecular could load 19.58 DOX molecules, and the complex exhibited sustained and pH-sensitive release behavior. PF127-PAMAM/DOX exhibited weaker cytotoxicity than free DOX in MCF-7 cells; while the complex showed much stronger reverse effect of drug resistance in MCF-7/ADR cells, and resistance reversion index (RRI) was as high as 33.15.
authors.forename: Zhuo-Jun
authors.initials: ZJ
authors.lastname: Gu
authors.forename: Meng
authors.initials: M
authors.lastname: Wang
authors.forename: Qiong-Yan
authors.initials: QY
authors.lastname: Fang
authors.forename: Cheng-Run
authors.initials: CR
authors.lastname: Wang
authors.forename: Huai-Yu
authors.initials: HY
authors.lastname: Zheng
mesh_terms: D050091:Dendrimers / Q000494:pharmacology*; D004317:Doxorubicin / Q000494:pharmacology*; D006801:Humans; D061986:MCF-7 Cells / Q000187:drug effects; D020442:Poloxamer / Q000494:pharmacology*
chemical_list: D050091:Dendrimers; C104700:PAMAM Starburst; D020442:Poloxamer; D004317:Doxorubicin