title: Hyperbaric oxygenation modulates vascular reactivity to angiotensin-(1-7) in diabetic rats: potential role of epoxyeicosatrienoic acids.
abstract: Previously, a facilitating effect of hyperbaric oxygenation (HBO₂) on aortic ring responses to angiotensin-(1-7) in healthy rats was reported, with epoxyeicosatrienoic acids (EETs) possibly playing an important role. The aim of this study was to assess whether HBO₂ exerts similar effects in diabetic rats and to further explore the role of specific cytochrome P450 (CYP) enzymes in changes induced by HBO₂. Aortic relaxation to angiotensin-(1-7) was significantly higher in HBO₂ diabetic rats compared to control diabetic rats, while HBO₂ had no effect on angiotensin II contraction. N-methylsulphonyl-6-(2-propargyloxyphenyl/hexanamide inhibited the facilitation of angiotensin-(1-7) responses in HBO₂ rats, suggesting an important role of EETs in this modulation. mRNA expression of CYP2J3 and protein expression of CYP2C11 were significantly upregulated in HBO₂ diabetic rats, whereas CYP4A1, CYP4A2 and CYP4A3 mRNA and CYP2J3 protein expression was similar between groups. Mean arterial pressure, ferric reducing ability of plasma and Thiobarbituric Acid Reactive Substances levels and serum angiotensin-(1-7) concentrations were not significantly changed.
authors.affiliation: Faculty of Medicine, University of Osijek, Osijek, Croatia.
authors.forename: Aleksandar
authors.initials: A
authors.lastname: Kibel
authors.affiliation: Faculty of Medicine, University of Osijek, Osijek, Croatia.
authors.forename: Sanja
authors.initials: S
authors.lastname: Novak
authors.affiliation: Faculty of Medicine, University of Osijek, Osijek, Croatia.
authors.forename: Anita
authors.initials: A
authors.lastname: Cosic
authors.affiliation: Faculty of Medicine, University of Osijek, Osijek, Croatia.
authors.forename: Zrinka
authors.initials: Z
authors.lastname: Mihaljevic
authors.affiliation: Department of Biochemistry, University of Texas Southwestern Medical Center, Dallas, TX, USA.
authors.forename: John R
authors.initials: JR
authors.lastname: Falck
authors.affiliation: Faculty of Medicine, University of Osijek, Osijek, Croatia ines.drenjancevic@mefos.hr.
authors.forename: Ines
authors.initials: I
authors.lastname: Drenjancevic
mesh_terms: D015126:8,11,14-Eicosatrienoic Acid / Q000031:analogs & derivatives* / Q000378:metabolism; D000577:Amides / Q000494:pharmacology; D000803:Angiotensin I / Q000097:blood / Q000494:pharmacology*; D000804:Angiotensin II / Q000494:pharmacology; D000818:Animals; D001013:Aorta, Thoracic / Q000187:drug effects / Q000378:metabolism / Q000503:physiopathology; D001189:Aryl Hydrocarbon Hydroxylases / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D003577:Cytochrome P-450 Enzyme System / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D000072467:Cytochrome P450 Family 2; D003922:Diabetes Mellitus, Type 1 / Q000150:complications / Q000378:metabolism / Q000503:physiopathology / Q000628:therapy*; D003925:Diabetic Angiopathies / Q000517:prevention & control*; D004790:Enzyme Induction; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006931:Hyperbaric Oxygenation / Q000009:adverse effects; D008297:Male; D018384:Oxidative Stress; D010446:Peptide Fragments / Q000097:blood / Q000494:pharmacology*; D017207:Rats, Sprague-Dawley; D039181:Steroid 16-alpha-Hydroxylase / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D014655:Vascular Resistance / Q000187:drug effects*; D014661:Vasoconstriction / Q000187:drug effects; D014664:Vasodilation / Q000187:drug effects; D014665:Vasodilator Agents / Q000097:blood / Q000494:pharmacology*
chemical_list: D000577:Amides; D004791:Enzyme Inhibitors; C121756:N-methylsulfonyl-6-(2-propargyloxyphenyl)hexanamide; D010446:Peptide Fragments; D014665:Vasodilator Agents; D000804:Angiotensin II; D003577:Cytochrome P-450 Enzyme System; D000803:Angiotensin I; D001189:Aryl Hydrocarbon Hydroxylases; C450279:CYP2C11 protein, rat; C106098:Cyp2j3 protein, rat; D000072467:Cytochrome P450 Family 2; D039181:Steroid 16-alpha-Hydroxylase; D015126:8,11,14-Eicosatrienoic Acid; C118790:angiotensin I (1-7)
keywords: Hyperbaric oxygen therapy; angiotensin II; angiotensin-(1-7); epoxyeicosatrienoic acids