title: Indomethacin can downregulate the levels of inflammatory mediators in the hippocampus of rats submitted to pilocarpine-induced status epilepticus.
abstract: OBJECTIVE
  Refractory status epilepticus is one of the most life-threatening neurological emergencies and is characterized by high morbidity and mortality. Additionally, the use of anti-inflammatory drugs during this period is very controversial. Thus, this study has been designed to analyze the effect of a low dose of indomethacin (a COX inhibitor) on the expression of inflammatory molecules.
  
  
  METHODS
  The hippocampus of rats submitted to pilocarpine-induced long-lasting status epilepticus was analyzed to determine the expression of inflammatory molecules with RT-PCR and immunohistochemistry.
  
  
  RESULTS
  Compared with controls, reduced levels of the kinin B2 receptors IL1β and TNFα were found in the hippocampus of rats submitted to long-lasting status epilepticus and treated with indomethacin.
  
  
  CONCLUSIONS
  These data show that low doses of indomethacin could be employed to minimize inflammation during long-lasting status epilepticus.
authors.affiliation: Departamento de Neurologia/Neurocirurgia, Universidade Federal de São Paulo, São Paulo, SP, Brazil.
authors.forename: Michele Juliane
authors.initials: MJ
authors.lastname: Vieira
authors.affiliation: Departamento de Neurologia/Neurocirurgia, Universidade Federal de São Paulo, São Paulo, SP, Brazil.
authors.forename: Sandra Regina
authors.initials: SR
authors.lastname: Perosa
authors.affiliation: Laboratório de Patologia Molecular, Faculdade de Ciências da Saúde, Universidade de Brasília, Brasilia, DF, Brazil.
authors.forename: Gustavo Adolfo
authors.initials: GA
authors.lastname: Argaãaraz
authors.affiliation: Programa de Pós-Graduação em Ciências da Reabilitação, Departamento de Educação Física, Universidade Nove de Julho, São Paulo, SP, Brazil.
authors.forename: José Antônio
authors.initials: JA
authors.lastname: Silva
authors.affiliation: Departamento de Neurologia/Neurocirurgia, Universidade Federal de São Paulo, São Paulo, SP, Brazil.
authors.forename: Esper Abrão
authors.initials: EA
authors.lastname: Cavalheiro
authors.affiliation: Departamento de Neurologia/Neurocirurgia, Universidade Federal de São Paulo, São Paulo, SP, Brazil.
authors.forename: Maria da
authors.initials: Md
authors.lastname: Graça Naffah-Mazzacoratti
mesh_terms: D000818:Animals; D016861:Cyclooxygenase Inhibitors / Q000494:pharmacology*; D004195:Disease Models, Animal; D015536:Down-Regulation / Q000187:drug effects; D006624:Hippocampus / Q000187:drug effects*; D007213:Indomethacin / Q000494:pharmacology*; D053583:Interleukin-1beta / Q000032:analysis / Q000187:drug effects; D008297:Male; D015846:Monokines / Q000032:analysis / Q000187:drug effects*; D010862:Pilocarpine; D017208:Rats, Wistar; D043783:Receptor, Bradykinin B1 / Q000032:analysis / Q000187:drug effects; D043782:Receptor, Bradykinin B2 / Q000032:analysis / Q000187:drug effects; D018002:Receptors, Bradykinin / Q000032:analysis / Q000187:drug effects*; D013226:Status Epilepticus / Q000139:chemically induced / Q000188:drug therapy*; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis / Q000187:drug effects
chemical_list: D016861:Cyclooxygenase Inhibitors; D053583:Interleukin-1beta; D015846:Monokines; D043783:Receptor, Bradykinin B1; D043782:Receptor, Bradykinin B2; D018002:Receptors, Bradykinin; D014409:Tumor Necrosis Factor-alpha; D010862:Pilocarpine; D007213:Indomethacin