title: Proteomic analysis of gliosomes from mouse brain: identification and investigation of glial membrane proteins.
abstract: Astrocytes are being increasingly recognized as crucial contributors to neuronal function at synapses, axons, and somas. Reliable methods that can provide insight into astrocyte proteins at the neuron-astrocyte functional interface are highly desirable. Here, we conducted a mass spectrometry analysis of Percoll gradient-isolated gliosomes, a viable preparation of glial subcellular particles often used to study mechanisms of astrocytic transmitter uptake and release and their regulation. Gliosomes were compared with synaptosomes, a preparation containing the neurotransmitter release machinery, and, accordingly, synaptosomes were enriched for proteins involved in synaptic vesicle-mediated transport. Interestingly, gliosome preparations were found to be enriched for different classes of known astrocyte proteins, such as VAMP3 (involved in astrocyte exocytosis), Ezrin (perisynaptic astrocyte cytoskeletal protein), and Basigin (astrocyte membrane glycoprotein), as well as for G-protein-mediated signaling proteins. Mass spectrometry data are available via ProteomeXchange with the identifier PXD001375. Together, these data provide the first detailed description of the gliosome proteome and show that gliosomes can be a useful preparation to study glial membrane proteins and associated processes.
authors.affiliation: Department of Molecular & Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Neuroscience Campus Amsterdam, VU University Amsterdam , 1081 HV Amsterdam, The Netherlands.
authors.forename: Karen E
authors.initials: KE
authors.lastname: Carney
authors.forename: Marco
authors.initials: M
authors.lastname: Milanese
authors.forename: Pim
authors.initials: P
authors.lastname: van Nierop
authors.forename: Ka Wan
authors.initials: KW
authors.lastname: Li
authors.forename: Stéphane H R
authors.initials: SH
authors.lastname: Oliet
authors.forename: August B
authors.initials: AB
authors.lastname: Smit
authors.forename: Giambattista
authors.initials: G
authors.lastname: Bonanno
authors.forename: Mark H G
authors.initials: MH
authors.lastname: Verheijen
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000378:metabolism*; D001921:Brain / Q000166:cytology / Q000378:metabolism*; D063990:Gene Ontology; D008565:Membrane Proteins / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism; D020543:Proteome / Q000235:genetics / Q000378:metabolism*; D040901:Proteomics; D013574:Synaptosomes / Q000378:metabolism; D053719:Tandem Mass Spectrometry
chemical_list: D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D020543:Proteome
keywords: Astrocyte proteins; gliosome; mass spectrometry; membrane proteins; synaptosome