title: [Collection of tracheal aspirate: safety and microbiological concordance between two techniques].
abstract: OBJECTIVE
  To evaluate the safety of the performance of the traditional and protected collection techniques of tracheal aspirate and to identify qualitative and quantitative agreement of the results of microbiological cultures between the techniques.
  
  
  METHODS
  Clinical, prospective, comparative, single-blind research. The sample was composed of 54 patients of >18 years of age, undergoing invasive mechanical ventilation for a period of ≥ 48 hours and with suspected Ventilator Associated Pneumonia. The two techniques were implemented in the same patient, one immediately after the other, with an order of random execution, according to randomization by specialized software.
  
  
  RESULTS
  No significant events occurred oxygen desaturation, hemodynamic instability or tracheobronchial hemorrhage (p<0.05) and, although there were differences in some strains, there was qualitative and quantitative agreement between the techniques (p<0.001).
  
  
  CONCLUSIONS
  Utilization of the protected technique provided no advantage over the traditional and execution of both techniques was safe for the patient.
authors.affiliation: Federal University of Mato Grosso do Sul, Campo Grande, MS, Brazil.
authors.forename: Oleci Pereira
authors.initials: OP
authors.lastname: Frota
authors.affiliation: Federal University of Mato Grosso do Sul, Campo Grande, MS, Brazil.
authors.forename: Adriano Menis
authors.initials: AM
authors.lastname: Ferreira
authors.affiliation: Nursing Course, Federal University of Mato Grosso do Sul, Coxim, MS, Brazil.
authors.forename: Larissa da Silva
authors.initials: Lda S
authors.lastname: Barcelos
authors.affiliation: College of Odontology of Ribeirão Preto, University of Sao Paulo, Ribeirão Preto, SP, Brazil.
authors.forename: Evandro
authors.initials: E
authors.lastname: Watanabe
authors.affiliation: Federal University of Mato Grosso do Sul, University of São Paulo, Campo Grande, MS, Brazil.
authors.forename: Nádia Cristina Pereira
authors.initials: NC
authors.lastname: Carvalho
authors.affiliation: Nursing Course, Federal University of Mato Grosso do Sul, Três Lagoas, MS, Brazil.
authors.forename: Marcelo Alessandro
authors.initials: MA
authors.lastname: Rigotti
mesh_terms: D001826:Body Fluids / Q000382:microbiology*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D011446:Prospective Studies; D016037:Single-Blind Method; D013048:Specimen Handling / Q000009:adverse effects / Q000379:methods*; D014132:Trachea