title: Expression and cellular distribution of estrogen and progesterone receptors and the real-time proliferation of porcine cumulus cells.
abstract: Although the expression of estrogen and progesterone receptors within porcine ovary and cumulus-oocyte complexes (COCs) is well recognized, still little information is known regarding expression of the progesterone receptor (PGR), PGR membrane component 1 (PGRMC1) and of estrogen-related receptors (ERRγ and ERRβ/γ) in separated cumulus cells in relation to real-time proliferation. In this study, a model of oocytes-separated cumulus cells was used to analyze the cell proliferation index and the expression PGR, PGRMC1 and of ERRγ and ERRβ/γ during 96-h cultivation in vitro using real-time quantitative PCR (qRT-PCR) and confocal microscopic observation. We found that PGR protein expression was increased at 0 h, compared with PGR protein expression after 96 h of culture (P < 0.001). The expression of PGRMC1, ERRγ and ERRβ/γ was unchanged. After using qRT-PCR we did not found statistical differences in expression of PGR, PGRMC1, ERRγ and ERRβ/γ during 96 h of cumulus cells in vitro culture (IVC). We supposed that the differential expression of the PGR protein at 0 h and after 96 h is related to a time-dependent down-regulation, which may activate a negative feedback. The distribution of PGR, PGRMC1 proteins may be linked with the translocation of receptors to the cytoplasm after the membrane binding of respective agonists and intra-cytoplasmic signal transduction. Furthermore, cumulus cells analyzed at 0 h were characterized by decreased proliferation index, whereas those after 96 h of culture revealed a significant increase of proliferation index, which may be associated with differentiation/luteinization of these cells during real-time proliferation.
authors.affiliation: Department of Histology and Embryology,Department of Anatomy,PoznanUniversity of Medical Sciences,6 Swiecickiego St.,60-781 Poznan,Poland.
authors.forename: Bartosz
authors.initials: B
authors.lastname: Kempisty
authors.affiliation: Department of Histology and Embryology,Poznan University of Medical Sciences,6 Swiecickiego St. 60-781 Poznan,Poland.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Ziółkowska
authors.affiliation: Department of Histology and Embryology,Poznan University of Medical Sciences,6 Swiecickiego St. 60-781 Poznan,Poland.
authors.forename: Sylwia
authors.initials: S
authors.lastname: Ciesiółka
authors.affiliation: Department of Toxicology,Poznan University of Medical Sciences,30 Dojazd St. 60-631 Poznan,Poland.
authors.forename: Hanna
authors.initials: H
authors.lastname: Piotrowska
authors.affiliation: Institute of Veterinary Sciences,Poznan University of Life Sciences,52 Wojska Polskiego St. 60-628,Poznan,Poland.
authors.forename: Paweł
authors.initials: P
authors.lastname: Antosik
authors.affiliation: Institute of Veterinary Sciences,Poznan University of Life Sciences,52 Wojska Polskiego St. 60-628,Poznan,Poland.
authors.forename: Dorota
authors.initials: D
authors.lastname: Bukowska
authors.affiliation: Institute of Reproductive Biology,Department of Experimental Reproductive Biology,Leibniz Institute for Farm Animal Biology,Dummerstorf,Germany.
authors.forename: Klaus P
authors.initials: KP
authors.lastname: Brüssow
authors.affiliation: Department of Histology and Embryology,Poznan University of Medical Sciences,6 Swiecickiego St. 60-781 Poznan,Poland.
authors.forename: Michał
authors.initials: M
authors.lastname: Nowicki
authors.affiliation: Department of Histology and Embryology,Wroclaw Medical University,6a Chalubinskiego St.,50-368 Wroclaw,Poland.
authors.forename: Maciej
authors.initials: M
authors.lastname: Zabel
mesh_terms: D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism; D049109:Cell Proliferation; D002478:Cells, Cultured; D054885:Cumulus Cells / Q000166:cytology* / Q000378:metabolism*; D003593:Cytoplasm / Q000378:metabolism; D005260:Female; D009865:Oocytes / Q000166:cytology / Q000378:metabolism; D011960:Receptors, Estrogen / Q000378:metabolism*; D011980:Receptors, Progesterone / Q000378:metabolism*; D013552:Swine
chemical_list: D011960:Receptors, Estrogen; D011980:Receptors, Progesterone
keywords: Cumulus cells; Estrogen receptors; Porcine oocyte–cumulus complexes; Progesterone receptors; Proliferation index