title: Turn-on phosphorescent chemodosimeter for Hg2+ based on a cyclometalated Ir(III) complex and its application in time-resolved luminescence assays and live cell imaging.
abstract: A novel "turn-on" phosphorescent chemodosimeter based on a cyclometalated Ir(III) complex has been designed and synthesized, which displays high selectivity and sensitivity toward Hg(2+) in aqueous media with a broad pH range of 4-10. Furthermore, by time-resolved photoluminescence techniques, some interferences from the short-lived background fluorescence can be eliminated effectively and the signal-to-noise ratio of the emission detection can be improved distinctly by using the chemodosimeter. Finally, the chemodosimeter can be used to monitor Hg(2+) effectively in living cells by confocal luminescence imaging.
authors.affiliation: Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province and State Key Laboratory of Applied Organic Chemistry, College of Chemistry and Chemical Engineering, and ‡School of Life Sciences, Lanzhou University , Lanzhou 730000, People's Republic of China.
authors.forename: Jia-Xi
authors.initials: JX
authors.lastname: Ru
authors.forename: Li-Ping
authors.initials: LP
authors.lastname: Guan
authors.forename: Xiao-Liang
authors.initials: XL
authors.lastname: Tang
authors.forename: Wei
authors.initials: W
authors.lastname: Dou
authors.forename: Xiang
authors.initials: X
authors.lastname: Yao
authors.forename: Wan-Min
authors.initials: WM
authors.lastname: Chen
authors.forename: Ya-Ming
authors.initials: YM
authors.lastname: Liu
authors.forename: Guo-Lin
authors.initials: GL
authors.lastname: Zhang
authors.forename: Wei-Sheng
authors.initials: WS
authors.lastname: Liu
authors.forename: Yue
authors.initials: Y
authors.lastname: Meng
authors.forename: Chun-Ming
authors.initials: CM
authors.lastname: Wang
mesh_terms: D002470:Cell Survival / Q000187:drug effects; D056831:Coordination Complexes / Q000138:chemical synthesis / Q000737:chemistry*; D004305:Dose-Response Relationship, Drug; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007495:Iridium / Q000737:chemistry*; D008163:Luminescent Measurements; D008628:Mercury / Q000032:analysis*; D015394:Molecular Structure; D011789:Quantum Theory; D013329:Structure-Activity Relationship; D013997:Time Factors; D014407:Tumor Cells, Cultured
chemical_list: D056831:Coordination Complexes; D007495:Iridium; D008628:Mercury