title: Diadenosine triphosphate is a novel factor which in combination with cyclodextrins synergistically enhances the biosynthesis of trans-resveratrol in Vitis vinifera cv. Monastrell suspension cultured cells.
abstract: Dinucleoside polyphosphates are considered as signal molecules that may evoke response of plant cells to stress. Other compounds whose biological effects have been recognized are cyclodextrins. They are cyclic oligosaccharides that chemically resemble the alkyl-derived pectic oligosaccharides naturally released from the cell walls during fungal attack, and they act as true elicitors, since, when added to plant cell culture, they induce the expression of genes involved in some secondary metabolism pathways. Previously, we demonstrated that some dinucleoside polyphosphates triggered the biosynthesis of enzymes involved in the phenylpropanoid pathway in Arabidopsis thaliana. In Vitis vinifera suspension cultured cells, cyclodextrins were shown to enhance the accumulation of trans-resveratrol, one of the basic units of the stilbenes derived from the phenylpropanoid pathway. Here, we show that diadenosine triphosphate, applied alone or in combination with cyclodextrins to the grapevine suspension-cultured cells, increased the transcript level of genes encoding key phenylpropanoid-pathway enzymes as well as the trans-resveratrol production inside cells and its secretion into the extracellular medium. In the latter case, these two compounds acted synergistically. However, the accumulation of trans-resveratrol and its glucoside trans-piceid inside cells were stimulated much better by diadenosine triphosphate than by cyclodextrins.
authors.affiliation: Department of Plant Physiology, Poznań University of Life Sciences, 35 Wołyńska Street, 60-637 Poznań, Poland; Departamento de Biología Vegetal, Facultad de Biología, Campus de Espinardo, Universidad de Murcia, 30100, Spain. Electronic address: gospi@up.poznan.pl.
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Pietrowska-Borek
authors.affiliation: Department of Plant Physiology, Poznań University of Life Sciences, 35 Wołyńska Street, 60-637 Poznań, Poland; Departamento de Biología Vegetal, Facultad de Biología, Campus de Espinardo, Universidad de Murcia, 30100, Spain.
authors.forename: Łukasz
authors.initials: Ł
authors.lastname: Czekała
authors.affiliation: Departamento de Biología Vegetal, Facultad de Biología, Campus de Espinardo, Universidad de Murcia, 30100, Spain.
authors.forename: Sarai
authors.initials: S
authors.lastname: Belchí-Navarro
authors.affiliation: Departamento de Biología Vegetal, Facultad de Biología, Campus de Espinardo, Universidad de Murcia, 30100, Spain.
authors.forename: María Angeles
authors.initials: MA
authors.lastname: Pedreño
authors.affiliation: Department of Biochemistry and Biotechnology, Poznań University of Life Sciences, 11 Dojazd Street, 60-632 Poznań, Poland. Electronic address: guranow@up.poznan.pl.
authors.forename: Andrzej
authors.initials: A
authors.lastname: Guranowski
mesh_terms: D002478:Cells, Cultured; D003505:Cyclodextrins / Q000494:pharmacology*; D015226:Dinucleoside Phosphates / Q000494:pharmacology*; D004357:Drug Synergism; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D000077185:Resveratrol; D013267:Stilbenes / Q000378:metabolism*; D027843:Vitis / Q000187:drug effects* / Q000378:metabolism*
chemical_list: D003505:Cyclodextrins; D015226:Dinucleoside Phosphates; D013267:Stilbenes; C011440:diadenosine triphosphate; D000077185:Resveratrol
keywords: Cyclodextrin; Diadenosine triphosphate; Phenylpropanoid pathway; Vitis vinifera cv. Monastrell; trans-Resveratrol