title: [Osteogenic gene expression of human periodontal ligament stem cells during osteogenic induction].
abstract: OBJECTIVE
  To isolate and identify the human periodontal ligament stem cells, evaluate osteogenetic capacity, and investigate the changes of osteogenic bone related gene expression in mineralized medium at different times.
  
  
  METHODS
  PDLSCs were isolated by tissue culture and magnetic activated cell sorting. Immunofluorescence staining was used for identification. The general situation of osteogenesis was assessed with alkaline phosphatase (ALP) and alizarin red staining. Real-time PCR was used to detect the expression of genes in osteoinduction. SPSS12.0 software package was used for data analysis.
  
  
  RESULTS
  Tissue culture with magnetic cell sorting could isolate high-purity human periodontal ligament stem cells. During the osteogenic process, the expression of FoxO1 and Runx2 firstly increased and then decreased, ALP and OCN gene levels continued to increase.
  
  
  CONCLUSIONS
  Similar to osteogenesis, ALP, Runx2, FoxO1 and OCN are regularly expressed during osteogenic induction.
authors.affiliation: Department of Oral and Cranomaxillofacial Science, Ninth People's Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine; Shanghai Key Laboratory of Stomatology. Shanghai 200011, China.E-mail:zhaojingleiff@gmail.com.
authors.forename: Jing-lei
authors.initials: JL
authors.lastname: Zhao
authors.forename: Ling-yong
authors.initials: LY
authors.lastname: Jiang
authors.forename: Li-xia
authors.initials: LX
authors.lastname: Mao
authors.forename: Jia-qiang
authors.initials: JQ
authors.lastname: Liu
authors.forename: Bing
authors.initials: B
authors.lastname: Fang
mesh_terms: D000469:Alkaline Phosphatase; D002454:Cell Differentiation; D015870:Gene Expression; D006801:Humans; D010012:Osteogenesis; D010513:Periodontal Ligament; D013234:Stem Cells
chemical_list: D000469:Alkaline Phosphatase