title: Effects of cellular viscoelasticity in multiple-bond force spectroscopy.
abstract: Receptor-ligand bonds are often subjected to forces that regulate their detachment via modulating off-rates. Though the dynamics of detachment is primarily controlled by the physical chemistry of adhesion molecules cellular features such as cell deformability and microvillus viscoelasticity have been shown to have an effect on it as well. In this work, Monte Carlo simulation of the rupture of multiple receptor-ligand bonds between substrate and a polymorphonuclear leukocyte (PMN) cell suspended in a Newtonian fluid is performed. It is demonstrated via various micromechanical models of the PMN cell adhered to the substrate by multiple receptor-ligand bonds that viscous drag caused by relative motion of cell suspended in a Newtonian fluid and cellular viscoelasticity modulate transmission of an applied external load to receptor-ligand bonds. It is demonstrated that due to cellular viscoelasticity the instantaneous intermolecular bond force is lower than the instantaneous applied force. It is also demonstrated that due to cellular viscoelasticity, the mean intermolecular bond rupture forces are lowered while the mean bond lifetime increases.
authors.affiliation: Department of Mechanical Engineering, University of Maryland Baltimore County, 1000 Hilltop Circle, Baltimore, MD, 21250, USA, vkg@umbc.edu.
authors.forename: V K
authors.initials: VK
authors.lastname: Gupta
mesh_terms: D004548:Elasticity; D008962:Models, Theoretical; D009010:Monte Carlo Method; D013057:Spectrum Analysis / Q000379:methods*; D014783:Viscosity