title: Gene expression of porcine blastocysts from gilts fed organic or inorganic selenium and pyridoxine.
abstract: In this study, we determined how maternal dietary supplementation with pyridoxine combined with different sources of selenium (Se) affected global gene expression of porcine expanded blastocysts (PEB) during pregnancy. Eighteen gilts were randomly assigned to one of the three experimental diets (n=6 per treatment): i) basal diet without supplemental Se or pyridoxine (CONT); ii) CONT+0.3 mg/kg of Na-selenite and 10 mg/kg of HCl-pyridoxine (MSeB610); and iii) CONT+0.3 mg/kg of Se-enriched yeast and 10 mg/kg of HCl-pyridoxine (OSeB610). All gilts were inseminated at their fifth post-pubertal estrus and killed 5 days later for embryo harvesting. A porcine embryo-specific microarray was used to detect differentially gene expression between MSeB610 vs CONT, OSeB610 vs CONT, and OSeB610 vs MSeB610. CONT gilts had lower whole blood Se and erythrocyte pyridoxal-5-P concentrations than supplemented gilts (P<0.05). No treatment effect was observed on blood plasma Se-glutathione peroxidase activity (P=0.57). There were 10, 247, and 96 differentially expressed genes for MSeB610 vs CONT, OSeB610 vs CONT, and OSeB610 vs MSeB610 respectively. No specific biological process was associated with MSeB610 vs CONT. However, for OSeB610 vs CONT, upregulated genes were related with global protein synthesis but not to selenoproteins. The stimulation of some genes related with monooxygenase and thioredoxin families was confirmed by quantitative real-time RT-PCR. In conclusion, OSeB610 affects PEB metabolism more markedly than MSeB610. Neither Se sources with pyridoxine influenced the Se-glutathione peroxidase metabolic pathway in the PEB, but OSeB610 selectively stimulated genes involved with antioxidant defense.
authors.affiliation: Dairy and Swine Research and Development CentreAgriculture and Agri-Food Canada, 2000 College Street, Sherbrooke, Quebec, Canada J1M 0C8Department of AgriculturalFood and Nutritional Science, University of Alberta, Edmonton, Alberta, Canada T6G 2P5Department of Animal ScienceUniversidade Estadual de Londrina, Londrina, Paraná 86057-970, Brazil Dairy and Swine Research and Development CentreAgriculture and Agri-Food Canada, 2000 College Street, Sherbrooke, Quebec, Canada J1M 0C8Department of AgriculturalFood and Nutritional Science, University of Alberta, Edmonton, Alberta, Canada T6G 2P5Department of Animal ScienceUniversidade Estadual de Londrina, Londrina, Paraná 86057-970, Brazil.
authors.forename: B D
authors.initials: BD
authors.lastname: Dalto
authors.affiliation: Dairy and Swine Research and Development CentreAgriculture and Agri-Food Canada, 2000 College Street, Sherbrooke, Quebec, Canada J1M 0C8Department of AgriculturalFood and Nutritional Science, University of Alberta, Edmonton, Alberta, Canada T6G 2P5Department of Animal ScienceUniversidade Estadual de Londrina, Londrina, Paraná 86057-970, Brazil.
authors.forename: S
authors.initials: S
authors.lastname: Tsoi
authors.affiliation: Dairy and Swine Research and Development CentreAgriculture and Agri-Food Canada, 2000 College Street, Sherbrooke, Quebec, Canada J1M 0C8Department of AgriculturalFood and Nutritional Science, University of Alberta, Edmonton, Alberta, Canada T6G 2P5Department of Animal ScienceUniversidade Estadual de Londrina, Londrina, Paraná 86057-970, Brazil.
authors.forename: I
authors.initials: I
authors.lastname: Audet
authors.affiliation: Dairy and Swine Research and Development CentreAgriculture and Agri-Food Canada, 2000 College Street, Sherbrooke, Quebec, Canada J1M 0C8Department of AgriculturalFood and Nutritional Science, University of Alberta, Edmonton, Alberta, Canada T6G 2P5Department of Animal ScienceUniversidade Estadual de Londrina, Londrina, Paraná 86057-970, Brazil.
authors.forename: M K
authors.initials: MK
authors.lastname: Dyck
authors.affiliation: Dairy and Swine Research and Development CentreAgriculture and Agri-Food Canada, 2000 College Street, Sherbrooke, Quebec, Canada J1M 0C8Department of AgriculturalFood and Nutritional Science, University of Alberta, Edmonton, Alberta, Canada T6G 2P5Department of Animal ScienceUniversidade Estadual de Londrina, Londrina, Paraná 86057-970, Brazil.
authors.forename: G R
authors.initials: GR
authors.lastname: Foxcroft
authors.affiliation: Dairy and Swine Research and Development CentreAgriculture and Agri-Food Canada, 2000 College Street, Sherbrooke, Quebec, Canada J1M 0C8Department of AgriculturalFood and Nutritional Science, University of Alberta, Edmonton, Alberta, Canada T6G 2P5Department of Animal ScienceUniversidade Estadual de Londrina, Londrina, Paraná 86057-970, Brazil jacques.matte@agr.gc.ca.
authors.forename: J J
authors.initials: JJ
authors.lastname: Matte
mesh_terms: D000821:Animal Feed; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D015415:Biomarkers / Q000378:metabolism*; D001755:Blastocyst / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism*; D002478:Cells, Cultured; D019587:Dietary Supplements; D004622:Embryo, Mammalian / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D005260:Female; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000187:drug effects*; D020411:Oligonucleotide Array Sequence Analysis; D011247:Pregnancy; D011736:Pyridoxine / Q000494:pharmacology*; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012643:Selenium / Q000494:pharmacology*; D013552:Swine; D014803:Vitamin B Complex / Q000494:pharmacology
chemical_list: D000975:Antioxidants; D015415:Biomarkers; D012333:RNA, Messenger; D014803:Vitamin B Complex; D012643:Selenium; D011736:Pyridoxine