title: Regulation of PKC mediated signaling by calcium during visceral leishmaniasis.
abstract: Calcium is an ubiquitous cellular signaling molecule that controls a variety of cellular processes and is strictly maintained in the cellular compartments by the coordination of various Ca2+ pumps and channels. Two such fundamental calcium pumps are plasma membrane calcium ATPase (PMCA) and Sarco/endoplasmic reticulum calcium ATPase (SERCA) which play a pivotal role in maintaining intracellular calcium homeostasis. This intracellular Ca2+ homeostasis is often disturbed by the protozoan parasite Leishmania donovani, the causative organism of visceral leishmaniasis. In the present study we have dileneated the involvement of PMCA4 and SERCA3 during leishmaniasis. We have observed that during leishmaniasis, intracellular Ca2+ concentration was up-regulated and was further controlled by both PMCA4 and SERCA3. Inhibition of these two Ca2+-ATPases resulted in decreased parasite burden within the host macrophages due to enhanced intracellular Ca2+. Contrastingly, on the other hand, activation of PMCA4 was found to enhance the parasite burden. Our findings also highlighted the importance of Ca2+ in the modulation of cytokine balance during leishmaniasis. These results thus cumulatively suggests that these two Ca2+-ATPases play prominent roles during visceral leishmaniasis.
authors.affiliation: Division of Molecular Medicine, Bose Institute, Kolkata, West Bengal, India.
authors.forename: Nivedita
authors.initials: N
authors.lastname: Roy
authors.affiliation: Division of Molecular Medicine, Bose Institute, Kolkata, West Bengal, India.
authors.forename: Supriya
authors.initials: S
authors.lastname: Chakraborty
authors.affiliation: Division of Molecular Medicine, Bose Institute, Kolkata, West Bengal, India.
authors.forename: Bidisha
authors.initials: B
authors.lastname: Paul Chowdhury
authors.affiliation: Division of Molecular Medicine, Bose Institute, Kolkata, West Bengal, India.
authors.forename: Sayantan
authors.initials: S
authors.lastname: Banerjee
authors.affiliation: Division of Molecular Medicine, Bose Institute, Kolkata, West Bengal, India.
authors.forename: Kuntal
authors.initials: K
authors.lastname: Halder
authors.affiliation: Division of Molecular Medicine, Bose Institute, Kolkata, West Bengal, India.
authors.forename: Saikat
authors.initials: S
authors.lastname: Majumder
authors.affiliation: Division of Molecular Medicine, Bose Institute, Kolkata, West Bengal, India.
authors.forename: Subrata
authors.initials: S
authors.lastname: Majumdar
authors.affiliation: Division of Molecular Medicine, Bose Institute, Kolkata, West Bengal, India.
authors.forename: Parimal C
authors.initials: PC
authors.lastname: Sen
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism*; D020013:Calcium Signaling / Q000235:genetics; D006706:Homeostasis / Q000235:genetics; D006801:Humans; D007893:Leishmania donovani / Q000469:parasitology / Q000472:pathogenicity; D007898:Leishmaniasis, Visceral / Q000378:metabolism* / Q000469:parasitology; D008264:Macrophages / Q000378:metabolism / Q000469:parasitology; D053499:Plasma Membrane Calcium-Transporting ATPases / Q000235:genetics / Q000378:metabolism*; D053498:Sarcoplasmic Reticulum Calcium-Transporting ATPases / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction / Q000235:genetics
chemical_list: D053499:Plasma Membrane Calcium-Transporting ATPases; D053498:Sarcoplasmic Reticulum Calcium-Transporting ATPases; D002118:Calcium