title: Induction of microtubule damage in Allium cepa meristematic cells by pharmaceutical formulations of thiabendazole and griseofulvin.
abstract: Microtubules (MT) are formed by the assembly of α- and β-tubulins and MT-associated proteins. We characterized the effects of pharmaceutical formulations containing the microtubule disruptors thiabendazole (TBZ) and griseofulvin (GF) on the mitotic machinery of plant (A. cepa) meristematic cells. GF concentrations between 10 and 250 μg/ml were tested. GF induced mitotic index inhibition and genotoxic effects, including chromosome fragments, bridges, lagged chromosomes, C-metaphases, tripolar cell division, disorganized anaphases and nuclear abnormalities in interphase cells. Efects on the mitotic machinery were studied by direct immunofluorescence with β-tubulin labeling and by DNA counterstaining with 4',6-diamidino-2-phenylindole (DAPI). Exposure of meristematic root cells to TBZ or GF, 100 μg/ml, caused microtubular damage which led to abnormal MT arrays. Our results suggest that GF induces abnormalities in spindle symmetry/polarity, while TBZ causes chromosome missegregation, polyploidy, and lack of cytokinesis.
authors.affiliation: Grupo de Investigación en Biología Evolutiva (GIBE), Departamento de Ecología, Genética y Evolución, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires - IEGEBA (CONICET-UBA), Intendente Güiraldes 2160 - Ciudad Universitaria, C1428EGA Buenos Aires, Argentina. Electronic address: nandrioli@hotmail.com.
authors.forename: Nancy B
authors.initials: NB
authors.lastname: Andrioli
authors.affiliation: Cátedra de Citología, Facultad de Ciencias Naturales y Museo, Universidad Nacional de La Plata, Calle 64 N° 3, B1904AMA La Plata, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Argentina.
authors.forename: Sonia
authors.initials: S
authors.lastname: Soloneski
authors.affiliation: Cátedra de Citología, Facultad de Ciencias Naturales y Museo, Universidad Nacional de La Plata, Calle 64 N° 3, B1904AMA La Plata, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Argentina.
authors.forename: Marcelo L
authors.initials: ML
authors.lastname: Larramendy
authors.affiliation: Grupo de Investigación en Biología Evolutiva (GIBE), Departamento de Ecología, Genética y Evolución, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires - IEGEBA (CONICET-UBA), Intendente Güiraldes 2160 - Ciudad Universitaria, C1428EGA Buenos Aires, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Argentina.
authors.forename: Marta D
authors.initials: MD
authors.lastname: Mudry
mesh_terms: D000871:Anthelmintics / Q000494:pharmacology*; D000935:Antifungal Agents / Q000494:pharmacology*; D032461:Chromosomes, Plant / Q000235:genetics / Q000378:metabolism; D004249:DNA Damage; D006118:Griseofulvin / Q000494:pharmacology*; D018519:Meristem / Q000235:genetics / Q000378:metabolism*; D008677:Metaphase / Q000187:drug effects / Q000235:genetics; D008870:Microtubules / Q000235:genetics / Q000378:metabolism*; D019697:Onions / Q000166:cytology / Q000235:genetics / Q000378:metabolism*; D059828:Plant Cells / Q000378:metabolism; D011123:Polyploidy; D013827:Thiabendazole / Q000494:pharmacology*
chemical_list: D000871:Anthelmintics; D000935:Antifungal Agents; D006118:Griseofulvin; D013827:Thiabendazole
keywords: Allium cepa; Griseofulvin; Microtubule arrays; Thiabendazole; Tubulin