title: Neural cell injury microenvironment induces neural differentiation of human umbilical cord mesenchymal stem cells.
abstract: This study aimed to investigate the neural differentiation of human umbilical cord mesenchymal stem cells (hUCMSCs) under the induction of injured neural cells. After in vitro isolation and culture, passage 5 hUCMSCs were used for experimentation. hUCMSCs were co-cultured with normal or Aβ1-40-injured PC12 cells, PC12 cell supernatant or PC12 cell lysate in a Transwell co-culture system. Western blot analysis and flow cytometry results showed that choline acetyltransferase and microtubule-associated protein 2, a specific marker for neural cells, were expressed in hUCMSCs under various culture conditions, and highest expression was observed in the hUCMSCs co-cultured with injured PC12 cells. Choline acetyltransferase and microtubule-associated protein 2 were not expressed in hUCMSCs cultured alone (no treatment). Cell Counting Kit-8 assay results showed that hUCMSCs under co-culture conditions promoted the proliferation of injured PC12 cells. These findings suggest that the microenvironment during neural tissue injury can effectively induce neural cell differentiation of hUCMSCs. These differentiated hUCMSCs likely accelerate the repair of injured neural cells.
authors.affiliation: Department of Neurology, First People's Hospital of Shenyang, Shenyang 110041, Liaoning Province, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Zhou
authors.affiliation: Department of Neurology, First People's Hospital of Shenyang, Shenyang 110041, Liaoning Province, China.
authors.forename: Guoping
authors.initials: G
authors.lastname: Tian
authors.affiliation: Department of Neurology, First People's Hospital of Shenyang, Shenyang 110041, Liaoning Province, China.
authors.forename: Jinge
authors.initials: J
authors.lastname: Wang
authors.affiliation: First Affiliated Hospital of China Medical University, Shenyang 110001, Liaoning Province, China.
authors.forename: Xiaoguang
authors.initials: X
authors.lastname: Luo
authors.affiliation: College of Basic Medical Sciences, China Medical University, Shenyang 110001, Liaoning Province, China.
authors.forename: Siyang
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Liaoning Provincial Blood Center, Shenyang 110044, Liaoning Province, China.
authors.forename: Jianping
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Neurology, First People's Hospital of Shenyang, Shenyang 110041, Liaoning Province, China.
authors.forename: Li
authors.initials: L
authors.lastname: Li
authors.affiliation: Department of Neurology, First People's Hospital of Shenyang, Shenyang 110041, Liaoning Province, China.
authors.forename: Bing
authors.initials: B
authors.lastname: Xu
authors.affiliation: Department of Neurology, First People's Hospital of Shenyang, Shenyang 110041, Liaoning Province, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Zhu
authors.affiliation: Department of Neurology, First People's Hospital of Shenyang, Shenyang 110041, Liaoning Province, China.
authors.forename: Xia
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Neurology, First People's Hospital of Shenyang, Shenyang 110041, Liaoning Province, China.
authors.forename: Chunhong
authors.initials: C
authors.lastname: Jia
authors.affiliation: Department of Neurology, First People's Hospital of Shenyang, Shenyang 110041, Liaoning Province, China.
authors.forename: Weijin
authors.initials: W
authors.lastname: Zhao
authors.affiliation: Department of Neurology, First People's Hospital of Shenyang, Shenyang 110041, Liaoning Province, China.
authors.forename: Danyang
authors.initials: D
authors.lastname: Zhao
authors.affiliation: Department of Neurology, First People's Hospital of Shenyang, Shenyang 110041, Liaoning Province, China.
authors.forename: Aihua
authors.initials: A
authors.lastname: Xu
keywords: Transwell; co-culture; differentiation; induction; injured cell; microtubule-associated protein 2; neural cell; neural regeneration; regeneration; stem cell; umbilical cord mesenchymal stem cell