title: The ARC1 E3 ligase promotes a strong and stable self-incompatibility response in Arabidopsis species: response to the Nasrallah and Nasrallah commentary.
abstract: Following the identification of the male (S-locus Cysteine Rich/S-locus Protein 11) and female (S Receptor kinase [SRK]) factors controlling self-incompatibility in the Brassicaceae, research in this field has focused on understanding the nature of the cellular responses activated by these regulators. We previously identified the ARM Repeat Containing1 (ARC1) E3 ligase as a component of the SRK signaling pathway and demonstrated ARC1's requirement in the stigma for self-incompatible pollen rejection in Brassica napus, Arabidopsis lyrata, and Arabidopsis thaliana. Here, we discuss our findings on the role of ARC1 in reconstructing a strong and stable A. thaliana self-incompatibility phenotype, in the context of the putative issues outlined in a commentary by Nasrallah and Nasrallah. Additionally, with their proposed standardized strategy for studying self-incompatibility in A. thaliana, we offer our perspective on what constitutes a strong and stable self-incompatibility phenotype in A. thaliana and how this should be investigated and reported to the greater community.
authors.affiliation: Department of Cell and Systems Biology, University of Toronto, Toronto M5S 3B2, Canada d.goring@utoronto.ca.
authors.forename: Daphne R
authors.identifier: http://orcid.org/0000-0001-5295-4744
authors.initials: DR
authors.lastname: Goring
authors.affiliation: Department of Biology, New Mexico State University, Las Cruces, New Mexico 88003.
authors.forename: Emily
authors.initials: E
authors.lastname: Indriolo
authors.affiliation: Department of Biological Sciences, University of Calgary, Calgary T2N 1N4, Canada.
authors.forename: Marcus A
authors.initials: MA
authors.lastname: Samuel
mesh_terms: D017360:Arabidopsis / Q000235:genetics*; D029681:Arabidopsis Proteins / Q000235:genetics*; D035264:Flowers / Q000235:genetics; D018506:Gene Expression Regulation, Plant; D008957:Models, Genetic; D010641:Phenotype; D030821:Plants, Genetically Modified; D054817:Pollination / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D057945:Self-Fertilization / Q000235:genetics; D060436:Self-Incompatibility in Flowering Plants / Q000235:genetics*; D044767:Ubiquitin-Protein Ligases / Q000235:genetics*
chemical_list: D029681:Arabidopsis Proteins; D044767:Ubiquitin-Protein Ligases