title: Revisiting Corynebacterium glyciniphilum (ex Kubota et al., 1972) sp. nov., nom. rev., isolated from putrefied banana.
abstract: A strain of a species of the genus Corynebacterium, designated AJ 3170(T), was isolated during the 1980s from putrefied bananas. Since then, there have been no further updates on the description of the strain or its phylogenetic classification. However, phylogenetic analysis of this strain using 16S rRNA and in silico DNA-DNA hybridization has confirmed that it is a member of the genus Corynebacterium and that strain AJ 3170(T) clusters with Corynebacterium variabile DSM 44702(T), Corynebacterium terpenotabidum Y-11(T) and Corynebacterium nuruki S6-4(T) in one subgroup. Furthermore, a combination of enzymatic, chemical, and morphological characterization techniques was applied in order to describe strain AJ 3170(T) further. The strain grew well at pH values of 6-10 and at temperatures of 30-41 °C. The major fatty acids were C16 : 0 (42.15 %), C18 : 1ω9c (41.6 %) and C18 : 0 10-methyl (TBSA) (8.56 %). The whole-cell sugars were determined to comprise galactose, arabinose and ribose. On the basis of this phenotypic, chemotaxonomic and phylogenetic characterization, it is proposed that strain AJ 3170(T) represents a novel species, for which the name Corynebacterium glyciniphilum sp. nov. is proposed; the type strain is AJ 3170(T) ( = DSM 45795(T) = ATCC 21341(T)).
authors.affiliation: Microbial Genomics and Biotechnology, CeBiTec, Bielefeld University, D-33615 Bielefeld, Germany.
authors.forename: Arwa
authors.initials: A
authors.lastname: Al-Dilaimi
authors.affiliation: Proteome and Metabolome Research, Bielefeld University, D-33615 Bielefeld, Germany.
authors.forename: Hanna
authors.initials: H
authors.lastname: Bednarz
authors.affiliation: Microbial Genomics and Biotechnology, CeBiTec, Bielefeld University, D-33615 Bielefeld, Germany.
authors.forename: Alexander
authors.initials: A
authors.lastname: Lömker
authors.affiliation: Proteome and Metabolome Research, Bielefeld University, D-33615 Bielefeld, Germany.
authors.forename: Karsten
authors.initials: K
authors.lastname: Niehaus
authors.affiliation: Technology Platform Genomics, CeBiTec, Bielefeld University, D-33615 Bielefeld, Germany.|Microbial Genomics and Biotechnology, CeBiTec, Bielefeld University, D-33615 Bielefeld, Germany.
authors.forename: Jörn
authors.initials: J
authors.lastname: Kalinowski
authors.affiliation: Technology Platform Genomics, CeBiTec, Bielefeld University, D-33615 Bielefeld, Germany.|Microbial Genomics and Biotechnology, CeBiTec, Bielefeld University, D-33615 Bielefeld, Germany.
authors.forename: Christian
authors.initials: C
authors.lastname: Rückert
mesh_terms: D001482:Base Composition; D002241:Carbohydrates / Q000737:chemistry; D003352:Corynebacterium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D008969:Molecular Sequence Data; D028521:Musa / Q000382:microbiology*; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D002241:Carbohydrates; D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S