title: Characterization of a new maleimido functionalization of gold for surface plasmon resonance spectroscopy.
abstract: Para-maleimidophenyl (p-MP) modified gold surfaces have been prepared by one-step electrochemical deposition and used in surface plasmon resonance (SPR) studies. Therefore, a FITC mimotope peptide (MP1, 12 aa), a human mucin 1 epitope peptide (MUC, 9 aa) and a protein with their specific antibodies were used as model systems. The peptides were modified with an N-terminal cysteine for covalent and directed coupling to the maleimido functionalized surface by means of Michael addition. The coupling yield of the peptide, the binding characteristics of antibody and the unspecific adsorption of the analytes were investigated. The results expand the spectrum of biosensors usable with p-MP by widely used SPR and support its potential to be versatile for several electrochemical and optical biosensors. This allows the combination of an electrochemical and optical read-out for a broad variety of biomolecular interactions on the same chip.
authors.affiliation: Fraunhofer Institute for Biomedical Engineering IBMT, Branch Potsdam, Department of Molecular Bioanalytics and Bioelectronics, Am Mühlenberg 13, 14476, Potsdam, Germany; University of Potsdam, Institute of Biochemistry and Biology, Karl-Liebknecht-Straße 24/25, 14476, Potsdam, Germany.
authors.forename: Marc
authors.initials: M
authors.lastname: Hovestädt
authors.forename: Henry
authors.initials: H
authors.lastname: Memczak
authors.forename: Dennis
authors.initials: D
authors.lastname: Pleiner
authors.forename: Xin
authors.initials: X
authors.lastname: Zhang
authors.forename: Jörg
authors.initials: J
authors.lastname: Rappich
authors.forename: Frank F
authors.initials: FF
authors.lastname: Bier
authors.forename: Walter F M
authors.initials: WF
authors.lastname: Stöcklein
mesh_terms: D000906:Antibodies / Q000378:metabolism; D016650:Fluorescein-5-isothiocyanate / Q000378:metabolism; D006046:Gold / Q000737:chemistry*; D049452:Green Fluorescent Proteins / Q000378:metabolism; D006801:Humans; D055767:Immobilized Proteins / Q000378:metabolism; D007700:Kinetics; D008024:Ligands; D008301:Maleimides / Q000737:chemistry*; D010455:Peptides / Q000378:metabolism; D011485:Protein Binding; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D020349:Surface Plasmon Resonance / Q000379:methods*
chemical_list: D000906:Antibodies; D055767:Immobilized Proteins; D008024:Ligands; D008301:Maleimides; D010455:Peptides; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins; C043592:maleimide; D006046:Gold; D016650:Fluorescein-5-isothiocyanate
keywords: aryl diazonium salts; biosensor; cys-peptide; diazonium coupling; maleimidophenyl; surface plasmon resonance