title: Diagnostic Role of Salivary and GCF Nitrite, Nitrate and Nitric Oxide to Distinguish Healthy Periodontium from Gingivitis and Periodontitis.
abstract: Diagnosis of subclinical and early stage clinical periodontal dysfunction could prevent from further socioeconomic burden. The aim of this study was to assess the diagnostic applicability of nitric oxide and its end-metabolites in periodontal tissue health and disease. Forty-two patients were enrolled and divided into three groups according to gingivitis (GI) and clinical attachment level (CAL) indices: a healthy group (GI<1, CAL<1), b: gingivitis (GI>1, CAL>1) and c: periodontitis (CAL>1) with 14 patients in each group. Unstimulated saliva and gingival crevicular fluid (GCF) were collected. Samples were evaluated for nitrite, nitrate and total nitric oxide contents with the ELISA method. In addition, CAL, GI, plaque index (PI), decay, missing, filling (DMFT) and bleeding index (BI) scores were also recorded. Except for GCF nitrite content (P= 0.89), there was an increasing trend for measured biomarkers in both saliva and GCF (Periodontitis> gingivitis> healthy periodontium, P< 0.05). Data remained stable after simultaneous adjustment for DMFT and BI scores as confounding factors. Sensitivity, specificity, positive predictive value, negative predictive value, cut point and p- value were as the followings: GCF nitrate (0.71, 0.11, 0.29,0.43, 4.97, P= 0.04), nitric oxide GCF ( 0.64, 0.18, 0.28, 0.5, 10.12, P= 0.04), nitrite saliva (0.93, 0.96,0.93,0.96,123.48, P< 0.001), salivary nitrate (0.93, 0.96, 0.93, 0.96, 123.6, P< 0.001), salivary nitric oxide (0.93, 0.96, 0.93, 0.96, 246.65, P <0.001). Our results revealed that NO plays an important role in the process of destruction of periodontal tissues. Within the limitation of our study, detecting NO biomarker and its end metabolites in saliva is of more value to assess the periodontal health comparing to GCF.
authors.affiliation: Dental Materials Research Center, Dentistry School, Babol University of Medical Sciences, Babol, Iran.
authors.forename: Arash
authors.initials: A
authors.lastname: Poorsattar Bejeh-Mir
authors.affiliation: Social Determinants of Health Research Center, Babol University of Medical Sciences, Babol, Iran ; Biochemistry and Biophysics Department, Babol University of Medical Sciences, Babol, Iran.
authors.forename: Hadi
authors.initials: H
authors.lastname: Parsian
authors.affiliation: Private Practice, Mazandaran Province, Iran.
authors.forename: Maryam
authors.initials: M
authors.lastname: Akbari Khoram
authors.affiliation: Private Practice, Mazandaran Province, Iran.
authors.forename: Nafiseh
authors.initials: N
authors.lastname: Ghasemi
authors.affiliation: Non-Communicable Pediatric Disease Research Center, Babol University of Medical Sciences, Babol, Mazandaran Province, Iran.
authors.forename: Ali
authors.initials: A
authors.lastname: Bijani
authors.affiliation: Dental Materials Research Center, Periodontology and Implantology Department, Babol University of Medical Sciences, Babol, Iran.
authors.forename: Mahmoud
authors.initials: M
authors.lastname: Khosravi-Samani
keywords: Periodontitis; gingival crevicular fluid; gingivitis; nitric oxide; saliva