title: Silencing the gene encoding C/EBP homologous protein lessens acute brain injury following ischemia/reperfusion.
abstract: C/EBP homologous protein, an important transcription factor during endoplasmic reticulum stress, participates in cell apoptosis mediated by endoplasmic reticulum stress. Previous studies have shown that C/EBP homologous protein mediates nerve injury during Alzheimer's disease, subarachnoid hemorrhage and spinal cord trauma. In this study, we introduced C/EBP homologous protein short hairpin RNA into the brains of ischemia/reperfusion rat models via injection of lentiviral vector through the left lateral ventricle. Silencing C/EBP homologous protein gene expression significantly reduced cerebral infarction volume, decreased water content and tumor necrosis factor-α and interleukin-1β mRNA expression in brain tissues following infarction, diminished the number of TUNEL-positive cells in the infarct region, decreased caspase-3 protein content and increased Bcl-2 protein content. These results suggest that silencing C/EBP homologous protein lessens cell apoptosis and inflammatory reactions, thereby protecting nerves.
authors.affiliation: Department of Neurology, Bethune First Hospital, Jilin University, Changchun 130021, Jilin Province, China.
authors.forename: Fengzhang
authors.initials: F
authors.lastname: Wang
authors.affiliation: Department of Neonatology, Bethune First Hospital, Jilin University, Changchun 130021, Jilin Province, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Orthopedics, Jilin Hospital of Integrated Traditional and Western Medicine, Changchun 130021, Jilin Province, China.
authors.forename: Chunke
authors.initials: C
authors.lastname: He
authors.affiliation: Department of Infectious Diseases, Zibo First Hospital, Zibo 255200, Shandong Province, China.
authors.forename: Tingting
authors.initials: T
authors.lastname: Wang
authors.affiliation: Department of Cardiology, General Hospital of China National Petroleum Corporation in Jilin, Jilin 132021, Jilin Province, China.
authors.forename: Qiyan
authors.initials: Q
authors.lastname: Piao
authors.affiliation: Department of Neurology, Bethune First Hospital, Jilin University, Changchun 130021, Jilin Province, China.
authors.forename: Qun
authors.initials: Q
authors.lastname: Liu
keywords: Alzheimer’s disease; C/EBP homologous protein; cerebral infarction; endoplasmic reticulum stress; interleukin-1β; ischemia/reperfusion; neural regeneration; subarachnoid hemorrhage; tumor necrosis factor-α