title: The probiotic Bifidobacterium breve B632 inhibited the growth of Enterobacteriaceae within colicky infant microbiota cultures.
abstract: Infant colic is a common gastrointestinal disorder of newborns, mostly related to imbalances in the composition of gut microbiota and particularly to the presence of gas-producing coliforms and to lower levels of Bifidobacteria and Lactobacilli. Probiotics could help to contain this disturbance, with formulations consisting of Lactobacillus strains being the most utilized. In this work, the probiotic strain Bifidobacterium breve B632 that was specifically selected for its ability to inhibit gas-producing coliforms, was challenged against the Enterobacteriaceae within continuous cultures of microbiota from a 2-month-old colicky infant. As confirmed by RAPD-PCR fingerprinting, B. breve B632 persisted in probiotic-supplemented microbiota cultures, accounting for the 64% of Bifidobacteria at the steady state. The probiotic succeeded in inhibiting coliforms, since FISH and qPCR revealed that the amount of Enterobacteriaceae after 18 h of cultivation was 0.42 and 0.44 magnitude orders lower (P < 0.05) in probiotic-supplemented microbiota cultures than in the control ones. These results support the possibility to move to another level of study, that is, the administration of B. breve B632 to a cohort of colicky newborns, in order to observe the behavior of this strain in vivo and to validate its effect in colic treatment.
authors.affiliation: Dipartimento di Scienze della Vita, Università di Modena e Reggio Emilia, Viale G. Campi 183, 41125 Modena, Italy.
authors.forename: Marta
authors.identifier: 0000-0001-7924-1461
authors.initials: M
authors.lastname: Simone
authors.affiliation: Dipartimento di Scienze della Vita, Università di Modena e Reggio Emilia, Viale G. Campi 183, 41125 Modena, Italy.
authors.forename: Caterina
authors.identifier: 0000-0002-7528-2378
authors.initials: C
authors.lastname: Gozzoli
authors.affiliation: Dipartimento di Scienze della Vita, Università di Modena e Reggio Emilia, Viale G. Campi 183, 41125 Modena, Italy.
authors.forename: Andrea
authors.identifier: 0000-0003-2624-601X
authors.initials: A
authors.lastname: Quartieri
authors.affiliation: Dipartimento di Scienze Agrarie, Università di Bologna, Viale Fanin 44, 40127 Bologna, Italy.
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Mazzola
authors.affiliation: Dipartimento di Scienze Agrarie, Università di Bologna, Viale Fanin 44, 40127 Bologna, Italy.
authors.forename: Diana
authors.identifier: 0000-0002-0181-1572
authors.initials: D
authors.lastname: Di Gioia
authors.affiliation: Dipartimento di Scienze della Vita, Università di Modena e Reggio Emilia, Viale G. Campi 183, 41125 Modena, Italy.
authors.forename: Alberto
authors.identifier: 0000-0002-2350-8902
authors.initials: A
authors.lastname: Amaretti
authors.affiliation: Dipartimento di Scienze della Vita, Università di Modena e Reggio Emilia, Viale G. Campi 183, 41125 Modena, Italy.
authors.forename: Stefano
authors.identifier: 0000-0003-0944-6677
authors.initials: S
authors.lastname: Raimondi
authors.affiliation: Dipartimento di Scienze della Vita, Università di Modena e Reggio Emilia, Viale G. Campi 183, 41125 Modena, Italy.
authors.forename: Maddalena
authors.identifier: 0000-0002-5342-3950
authors.initials: M
authors.lastname: Rossi
mesh_terms: D001644:Bifidobacterium / Q000502:physiology*; D002478:Cells, Cultured; D003085:Colic / Q000382:microbiology*; D015169:Colony Count, Microbial; D004755:Enterobacteriaceae / Q000254:growth & development*; D005243:Feces / Q000382:microbiology; D005285:Fermentation; D041981:Gastrointestinal Tract / Q000382:microbiology; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007231:Infant, Newborn; D064307:Microbiota; D019936:Probiotics / Q000378:metabolism*; D013997:Time Factors