title: Calcium is involved in the R Mc1 (blb)-mediated hypersensitive response against Meloidogyne chitwoodi in potato.
abstract: Functional characterization of the Columbia root-knot nematode resistance gene R Mc1 ( blb ) in potato revealed the R gene-mediated resistance is dependent on a hypersensitive response and involves calcium. The resistance (R) gene R Mc1(blb) confers resistance against the plant-parasitic nematode, Meloidogyne chitwoodi. Avirulent and virulent nematodes were used to functionally characterize the R Mc1(blb)-mediated resistance mechanism in potato (Solanum tuberosum). Histological observations indicated a hypersensitive response (HR) occurred during avirulent nematode infection. This was confirmed by quantifying reactive oxygen species activity in response to avirulent and virulent M. chitwoodi. To gain an insight into the signal transduction pathways mediating the R Mc1(blb)-induced HR, chemical inhibitors were utilized. Inhibiting Ca(2+) channels caused a significant reduction in electrolyte leakage, an indicator of cell death. Labeling with a Ca(2+)-sensitive dye revealed high Ca(2+) levels in the root cells surrounding avirulent nematodes. Furthermore, the calcium-dependent protein kinase (CDPK), StCDPK4 had a higher transcript level in R Mc1(blb) potato roots infected with avirulent nematodes in comparison to roots infected with virulent M. chitwoodi. The results of this study indicate Ca(2+) plays a role in the R Mc1(blb)-mediated resistance against M. chitwoodi in potato.
authors.affiliation: Department of Plant Pathology, Washington State University, Pullman, WA, 99164, USA.
authors.forename: Laura J
authors.initials: LJ
authors.lastname: Davies
authors.forename: Charles R
authors.initials: CR
authors.lastname: Brown
authors.forename: Axel A
authors.initials: AA
authors.lastname: Elling
mesh_terms: D000818:Animals; D002118:Calcium / Q000276:immunology* / Q000378:metabolism; D002121:Calcium Channel Blockers / Q000494:pharmacology; D060467:Disease Resistance / Q000235:genetics / Q000276:immunology*; D004573:Electrolytes / Q000378:metabolism; D018506:Gene Expression Regulation, Plant / Q000276:immunology; D017343:Genes, Plant / Q000235:genetics / Q000276:immunology*; D006790:Host-Parasite Interactions / Q000276:immunology; D010935:Plant Diseases / Q000235:genetics / Q000276:immunology* / Q000469:parasitology; D010940:Plant Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D018517:Plant Roots / Q000235:genetics / Q000276:immunology / Q000469:parasitology; D011494:Protein Kinases / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000276:immunology / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction / Q000187:drug effects / Q000235:genetics / Q000276:immunology; D011198:Solanum tuberosum / Q000235:genetics / Q000276:immunology* / Q000469:parasitology; D014431:Tylenchoidea / Q000276:immunology* / Q000472:pathogenicity / Q000502:physiology; D014774:Virulence / Q000276:immunology; D017871:Calcium-Calmodulin-Dependent Protein Kinases
chemical_list: D002118:Calcium; D002121:Calcium Channel Blockers; D004573:Electrolytes; D010940:Plant Proteins; D011494:Protein Kinases; D017382:Reactive Oxygen Species; D017871:Calcium-Calmodulin-Dependent Protein Kinases