title: Metabolomic and lipidomic analysis of serum from mice exposed to an internal emitter, cesium-137, using a shotgun LC-MS(E) approach.
abstract: In this study ultra performance liquid chromatography (UPLC) coupled to time-of-flight mass spectrometry in the MS(E) mode was used for rapid and comprehensive analysis of metabolites in the serum of mice exposed to internal exposure by Cesium-137 ((137)Cs). The effects of exposure to (137)Cs were studied at several time points after injection of (137)CsCl in mice. Over 1800 spectral features were detected in the serum of mice in positive and negative electrospray ionization modes combined. Detailed statistical analysis revealed that several metabolites associated with amino acid metabolism, fatty acid metabolism, and the TCA cycle were significantly perturbed in the serum of (137)Cs-exposed mice compared with that of control mice. While metabolites associated with the TCA cycle and glycolysis increased in their serum abundances, fatty acids such as linoleic acid and palmitic acid were detected at lower levels in serum after (137)Cs exposure. Furthermore, phosphatidylcholines (PCs) were among the most perturbed ions in the serum of (137)Cs-exposed mice. This is the first study on the effects of exposure by an internal emitter in serum using a UPLC-MS(E) approach. The results have put forth a panel of metabolites, which may serve as potential serum markers to (137)Cs exposure.
authors.affiliation: Biochemistry and Molecular and Cellular Biology, Georgetown University , 3970 Reservoir Road NW, Washington, DC 20057, United States.
authors.forename: Maryam
authors.initials: M
authors.lastname: Goudarzi
authors.forename: Waylon M
authors.initials: WM
authors.lastname: Weber
authors.forename: Tytus D
authors.initials: TD
authors.lastname: Mak
authors.forename: Juijung
authors.initials: J
authors.lastname: Chung
authors.forename: Melanie
authors.initials: M
authors.lastname: Doyle-Eisele
authors.forename: Dunstana R
authors.initials: DR
authors.lastname: Melo
authors.forename: David J
authors.initials: DJ
authors.lastname: Brenner
authors.forename: Raymond A
authors.initials: RA
authors.lastname: Guilmette
authors.forename: Albert J
authors.initials: AJ
authors.lastname: Fornace
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000097:blood*; D001774:Blood Chemical Analysis / Q000379:methods; D002588:Cesium Radioisotopes / Q000633:toxicity*; D002851:Chromatography, High Pressure Liquid; D050356:Lipid Metabolism / Q000528:radiation effects*; D013058:Mass Spectrometry; D055442:Metabolome / Q000528:radiation effects*; D055432:Metabolomics / Q000379:methods; D051379:Mice; D010713:Phosphatidylcholines / Q000097:blood; D025341:Principal Component Analysis
chemical_list: D015415:Biomarkers; D002588:Cesium Radioisotopes; D010713:Phosphatidylcholines
keywords: Cesium-137; UPLC−MSE; internal emitter; lipidomics; mass spectrometry; metabolomics; radiation