title: A simple, rapid and low-cost staining method for gel-electrophoresis separated phosphoproteins via the fluorescent purpurin dye.
abstract: A novel fluorescence detection method for phosphoproteins in 1-D and 2-D SDS-PAGE by using purpurin is developed in this study. Phosphoproteins as low as 4-8 ng could be specifically detected by purpurin within 60 min, and the detection limit is similar to or better than that of Pro-Q Diamond staining. Only 2 steps (staining and destaining) are needed for purpurin staining without requiring excessive fixing and washing steps, and for single use, $0.8 is enough for purpurin staining. By comprehensively comparing with Pro-Q Diamond staining, it is concluded that purpurin staining is a simple, rapid and low-cost staining method for a broad application to the research of phosphoproteins.
authors.affiliation: School of Pharmaceutical Sciences, Key Laboratory of Biotechnology Pharmaceutical Engineering, Wenzhou Medical University, Wenzhou, Zhejiang, China. jin_litai@126.com luxh0393@163.com.
authors.forename: Weitao
authors.initials: W
authors.lastname: Cong
authors.forename: Jiayi
authors.initials: J
authors.lastname: Shen
authors.forename: Yuanhu
authors.initials: Y
authors.lastname: Xuan
authors.forename: Xinliang
authors.initials: X
authors.lastname: Zhu
authors.forename: Maowei
authors.initials: M
authors.lastname: Ni
authors.forename: Zhongxin
authors.initials: Z
authors.lastname: Zhu
authors.forename: Guoying
authors.initials: G
authors.lastname: Hong
authors.forename: Xianghong
authors.initials: X
authors.lastname: Lu
authors.forename: Litai
authors.initials: L
authors.lastname: Jin
mesh_terms: D000880:Anthraquinones / Q000737:chemistry*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D005456:Fluorescent Dyes / Q000737:chemistry*; D010750:Phosphoproteins / Q000737:chemistry*; D010766:Phosphorylation; D012680:Sensitivity and Specificity; D013194:Staining and Labeling / Q000191:economics / Q000379:methods
chemical_list: D000880:Anthraquinones; D005456:Fluorescent Dyes; D010750:Phosphoproteins; C511975:purpurin anthraquinone