title: The Laurdan spectral phasor method to explore membrane micro-heterogeneity and lipid domains in live cells.
abstract: In this method paper we describe the spectral phasor analysis applied to Laurdan emission for the assessment of the fluidity of different membranes in live cells. We first introduce the general context and then we show how to obtain the spectral phasor from data acquired using a commercial microscope.
authors.affiliation: Laboratory for Fluorescence Dynamics, Department of Biomedical Engineering, University of California, 3208 Natural Sciences II, Irvine, CA, 92697-2715, USA.
authors.forename: Ottavia
authors.initials: O
authors.lastname: Golfetto
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Hinde
authors.forename: Enrico
authors.initials: E
authors.lastname: Gratton
mesh_terms: D015081:2-Naphthylamine / Q000031:analogs & derivatives*; D000818:Animals; D002462:Cell Membrane / Q000737:chemistry* / Q000378:metabolism; D002772:Cholera Toxin / Q000737:chemistry; D057809:HEK293 Cells; D006801:Humans; D007848:Laurates; D008563:Membrane Lipids / Q000737:chemistry; D051379:Mice; D008967:Molecular Biology / Q000379:methods; D041681:NIH 3T3 Cells; D013050:Spectrometry, Fluorescence / Q000379:methods*
chemical_list: D007848:Laurates; D008563:Membrane Lipids; D002772:Cholera Toxin; D015081:2-Naphthylamine; C065580:laurdan