title: Caspase-3 activation is a critical determinant of genotoxic stress-induced apoptosis.
abstract: Apoptosis can be measured by number of methods by taking advantage of the morphological, biochemical, and molecular changes undergoing in a cell during this process. The best recognized biochemical hallmark of both early and late stages of apoptosis is the activation of cysteine proteases (caspases). Detection of active caspase-3 in cells and tissues is an important method for apoptosis induced by a wide variety of apoptotic signals. Most common assays for examining caspase-3 activation include immunostaining, immunoblotting for active caspase-3, colorimetric assays using fluorochrome substrates, as well as employing the fluorescein-labeled CaspaTag pan-caspase in situ detection kit.
authors.affiliation: Department of Cancer Biology, Lerner Research Institute, Cleveland Clinic Foundation, 9500 Euclid Ave. NB40, Cleveland, OH, 44195, USA.
authors.forename: Gaurav S
authors.initials: GS
authors.lastname: Choudhary
authors.forename: Sayer
authors.initials: S
authors.lastname: Al-Harbi
authors.forename: Alexandru
authors.initials: A
authors.lastname: Almasan
mesh_terms: D000818:Animals; D017209:Apoptosis; D053148:Caspase 3 / Q000032:analysis* / Q000378:metabolism*; D004249:DNA Damage; D004789:Enzyme Activation; D005434:Flow Cytometry / Q000379:methods*; D019793:Fluorescein; D005456:Fluorescent Dyes; D006801:Humans; D015151:Immunoblotting / Q000379:methods*; D007150:Immunohistochemistry / Q000379:methods*; D051379:Mice
chemical_list: D005456:Fluorescent Dyes; D053148:Caspase 3; D019793:Fluorescein