title: Taurine improves the wound healing process in cutaneous leishmaniasis in mice model, based on stereological parameters.
abstract: BACKGROUND
  Cutaneous Leishmaniasis is a self-limiting disease caused by protozoan parasites of the genus Leishmania, which affects the skin with full-thickness wounds, which are prone to scar formation even after treatment. Taurine (Tu) is one of the most abundant amino acids that has antioxidant and anti-inflammatory effects, which play an important role in the process of wound healing. Herein, we have investigated the effects of Tu on cutaneous Leishmaniasis wounds and L. major promastigotes.
  
  
  METHODS
  Eighteen mice were induced with Leishmaniasis wounds (with L. Major) on the base of their tails and divided into three groups, T1: Treated with Tu injection, T2: Treated with Tu gel, and C: No treatment. Treatments were carried out every 24 hours for 21 days. The volume densities of the collagen bundles and vessels, vessel's length density and diameter, and fibroblast populations were estimated by stereological methods. Flow cytometry was used in order to investigate the direct Tu effect on parasites. The Mann-Whitney U test was used and P ≤ 0.05 was considered to be statistically significant.
  
  
  RESULTS
  The numerical density of the fibroblasts, volume density of the collagen bundles, and length densities of the vessels in groups T1 and T2 were significantly higher than in group C (P < 0.05). The fibroblast numerical density of group T1 was higher than that of group T2 (P = 0.02). Incidentally, Tu had no direct effect on L. major parasites according to the flow cytometry analysis.
  
  
  CONCLUSIONS
  Tu showed the ability to improve the wound healing process and tissue regeneration although it had no direct anti-leishmaniasis effect.
authors.affiliation: Student Research Committee, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Soheil
authors.initials: S
authors.lastname: Ashkani-Esfahani
authors.affiliation: Student Research Committee, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Fariba
authors.initials: F
authors.lastname: Zarifi
authors.affiliation: Department of Parasitology and Mycology, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Qasem
authors.initials: Q
authors.lastname: Asgari
authors.affiliation: Student Research Committee, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Asal Zargari
authors.initials: AZ
authors.lastname: Samadnejad
authors.affiliation: Student Research Committee, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Shima
authors.initials: S
authors.lastname: Rafiee
authors.affiliation: Histomorphometry and Stereology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Ali
authors.initials: A
authors.lastname: Noorafshan
keywords: Cutaneous leishmaniasis; mice; stereology; taurine; wound healing