title: Laser spectrometry for multi-elemental imaging of biological tissues.
abstract: An increasing interest has arisen in research focused on metallic and organic ions that play crucial roles in both physiological and pathological metabolic processes. Current methods for the observation of trace elements in biological tissues at microscopic spatial resolution often require equipment with high complexity. We demonstrate a novel approach with an all-optical design and multi-elemental scanning imaging, which is unique among methods of elemental detection because of its full compatibility with standard optical microscopy. This approach is based on laser-induced breakdown spectroscopy (LIBS), which allows the elements in a tissue sample to be directly detected and quantified under atmospheric pressure. We successfully applied this method to murine kidneys with 10 µm resolution and a ppm-level detection limit to analyze the renal clearance of nanoparticles. These results offer new insight into the use of laser spectrometry in biomedical applications in the field of label-free elemental mapping of biological tissues.
authors.affiliation: 1] Institut Lumière Matière, UMR5306 Université Lyon 1-CNRS, Université de Lyon 69622 Villeurbanne cedex, France [2].
authors.forename: L
authors.initials: L
authors.lastname: Sancey
authors.affiliation: 1] Institut Lumière Matière, UMR5306 Université Lyon 1-CNRS, Université de Lyon 69622 Villeurbanne cedex, France [2].
authors.forename: V
authors.initials: V
authors.lastname: Motto-Ros
authors.affiliation: Université Joseph Fourier, Inserm U823, IAB, F-38000 Grenoble, France.
authors.forename: B
authors.initials: B
authors.lastname: Busser
authors.affiliation: Institut Lumière Matière, UMR5306 Université Lyon 1-CNRS, Université de Lyon 69622 Villeurbanne cedex, France.
authors.forename: S
authors.initials: S
authors.lastname: Kotb
authors.affiliation: Institut Lumière Matière, UMR5306 Université Lyon 1-CNRS, Université de Lyon 69622 Villeurbanne cedex, France.
authors.forename: J M
authors.initials: JM
authors.lastname: Benoit
authors.affiliation: Institut Lumière Matière, UMR5306 Université Lyon 1-CNRS, Université de Lyon 69622 Villeurbanne cedex, France.
authors.forename: A
authors.initials: A
authors.lastname: Piednoir
authors.affiliation: Institut Lumière Matière, UMR5306 Université Lyon 1-CNRS, Université de Lyon 69622 Villeurbanne cedex, France.
authors.forename: F
authors.initials: F
authors.lastname: Lux
authors.affiliation: Institut Lumière Matière, UMR5306 Université Lyon 1-CNRS, Université de Lyon 69622 Villeurbanne cedex, France.
authors.forename: O
authors.initials: O
authors.lastname: Tillement
authors.affiliation: Institut Lumière Matière, UMR5306 Université Lyon 1-CNRS, Université de Lyon 69622 Villeurbanne cedex, France.
authors.forename: G
authors.initials: G
authors.lastname: Panczer
authors.affiliation: Institut Lumière Matière, UMR5306 Université Lyon 1-CNRS, Université de Lyon 69622 Villeurbanne cedex, France.
authors.forename: J
authors.initials: J
authors.lastname: Yu
mesh_terms: D000818:Animals; D004396:Coloring Agents; D003952:Diagnostic Imaging; D007668:Kidney / Q000378:metabolism / Q000648:ultrastructure*; D051379:Mice; D053758:Nanoparticles / Q000737:chemistry; D014131:Trace Elements / Q000302:isolation & purification* / Q000378:metabolism
chemical_list: D004396:Coloring Agents; D014131:Trace Elements