title: Isolation and genetic analysis of an environmental bacteriophage: A 10-session laboratory series in molecular virology.
abstract: Bacterial viruses, otherwise known as bacteriophage (or phage), are some of the most abundant viruses found in the environment. They can be easily isolated from water or soil and are ideal for use in laboratory classrooms due to their ease of culture and inherent safety. Here, we describe a series of 10 laboratory exercises where students collect, isolate, and purify the genome of an environmental phage. Once the genome has been extracted, students then clone a fragment of their isolated phage genome into a plasmid and analyze its sequence to identify the phage in their original isolate. These exercises have been carefully designed to apply foundational concepts that will expose students to basic skills in microbiology, molecular biology, and bioinformatics.
authors.affiliation: Department of Biology at Sonoma State University, Rohnert Park, California, 94928.
authors.forename: Ryan P
authors.initials: RP
authors.lastname: Williamson
authors.forename: Brent T
authors.initials: BT
authors.lastname: Barker
authors.forename: Hamidou
authors.initials: H
authors.lastname: Drammeh
authors.forename: Jefferson
authors.initials: J
authors.lastname: Scott
authors.forename: Joseph
authors.initials: J
authors.lastname: Lin
mesh_terms: D001435:Bacteriophages / Q000235:genetics* / Q000302:isolation & purification*; D019295:Computational Biology / Q000193:education; D003479:Curriculum; D008967:Molecular Biology / Q000193:education*; D013663:Teaching; D014773:Virology / Q000193:education*
keywords: bacteriophage; laboratory exercises; molecular virology