title: High fat diet induces adhesion of platelets to endothelium in two models of dyslipidemia.
abstract: Cardiovascular diseases (CVD) represent about 30% of all global deaths. It is currently accepted that, in the atherogenic process, platelets play an important role, contributing to endothelial activation and modulation of the inflammatory phenomenon, promoting the beginning and formation of lesions and their subsequent thrombotic complications. The objective of the present work was to study using immunohistochemistry, the presence of platelets, monocytes/macrophages, and cell adhesion molecules (CD61, CD163, and CD54), in two stages of the atheromatous process. CF-1 mice fed a fat diet were used to obtain early stages of atheromatous process, denominated early stage of atherosclerosis, and ApoE(-/-) mice fed a fat diet were used to observe advanced stages of atherosclerosis. The CF-1 mice model presented immunostaining on endothelial surface for all three markers studied; the advanced atherosclerosis model in ApoE(-/-) mice also presented granular immunostaining on lesion thickness, for the same markers. These results suggest that platelets participate in atheromatous process from early stages to advance d stages. High fat diet induces adhesion of platelets to endothelial cells in vivo. These findings support studying the participation of platelets in the formation of atheromatous plate.
authors.affiliation: Departamento de Bioquímica Clínica e Inmunohematología, Facultad de Ciencias de la Salud, Universidad de Talca, P.O. Box 747, Talca, Chile.
authors.forename: Jaime
authors.initials: J
authors.lastname: Gonzalez
authors.affiliation: Departamento de Estomatología, Facultad de Ciencias de la Salud, Universidad de Talca, Avenida Lircay s/n, Talca, Chile.
authors.forename: Wendy
authors.initials: W
authors.lastname: Donoso
authors.affiliation: Departamento de Bioquímica Clínica e Inmunohematología, Facultad de Ciencias de la Salud, Universidad de Talca, P.O. Box 747, Talca, Chile.
authors.forename: Natalia
authors.initials: N
authors.lastname: Díaz
authors.affiliation: Departamento de Ciencias Básicas Biomédicas, Facultad de Ciencias de la Salud, Universidad de Talca, Avenida Lircay s/n, Talca, Chile.
authors.forename: María Eliana
authors.initials: ME
authors.lastname: Albornoz
authors.affiliation: Departamento de Bioquímica Clínica e Inmunohematología, Facultad de Ciencias de la Salud, Universidad de Talca, P.O. Box 747, Talca, Chile.
authors.forename: Ricardo
authors.initials: R
authors.lastname: Huilcaman
authors.affiliation: Facultad de Medicina, Universidad Católica del Maule, Avenida San Miguel 3605, 3480112 Talca, Chile.
authors.forename: Erik
authors.initials: E
authors.lastname: Morales
authors.affiliation: Departamento de Bioquímica Clínica e Inmunohematología, Facultad de Ciencias de la Salud, Universidad de Talca, P.O. Box 747, Talca, Chile ; Centro de Estudios en Alimentos Procesados (CEAP), Conicyt-Regional, Gore Maule, R09I2001, Avenida San Miguel 3425, 3480137 Talca, Chile ; Programa Investigación de Excelencia Interdisciplinario en Envejecimiento Saludable PIEI-ES, Universidad de Talca, Avenida Lircay s/n, Talca, Chile.
authors.forename: Rodrigo
authors.identifier: 0000-0003-4870-9660
authors.initials: R
authors.lastname: Moore-Carrasco
mesh_terms: D000818:Animals; D001057:Apolipoproteins E / Q000378:metabolism*; D050197:Atherosclerosis / Q000473:pathology*; D001792:Blood Platelets / Q000473:pathology*; D002448:Cell Adhesion; D059305:Diet, High-Fat / Q000009:adverse effects*; D004195:Disease Models, Animal; D018450:Disease Progression; D050171:Dyslipidemias / Q000473:pathology*; D004727:Endothelium / Q000473:pathology*; D007150:Immunohistochemistry; D007249:Inflammation / Q000473:pathology*; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout
chemical_list: D001057:Apolipoproteins E