title: Autophagy activity in pulmonary metastatic tumor tissues from colorectal cancer: a pilot study.
abstract: OBJECTIVE
  Autophagy has been reported to be involved in treatment failure in tumor. We aimed to evaluate autophagy activity in tumor tissue and compare them between the recurrence and non-recurrence groups.
  
  
  METHODS
  We analyzed expressions of autophagy-related proteins in tumor tissues which were obtained from pulmonary metastases of colorectal cancer patients by Western blot. We also analyzed autophagosomes by transmission electron microscopy.
  
  
  RESULTS
  Tumor tissues from recurrence group showed increased levels of LC3B-II, decreased levels of p62/SQSTM1, and also a marked accumulation of autophagosomes compared with tissues from non-recurrence group.
  
  
  CONCLUSIONS
  The present study suggests that autophagy may be associated with treatment failure of metastatic colorectal cancer.
authors.affiliation: Department of Internal Medicine, Yonsei University College of Medicine, Seoul, Korea.
authors.forename: Young Sam
authors.initials: YS
authors.lastname: Kim
authors.affiliation: Department of Internal Medicine, Yonsei University College of Medicine, Seoul, Korea.
authors.forename: Ju Hye
authors.initials: JH
authors.lastname: Shin
authors.affiliation: Department of Thoracic and Cardiovascular Surgery, Yonsei University College of Medicine, Seoul, Korea.
authors.forename: Mi Kyung
authors.initials: MK
authors.lastname: Bae
authors.affiliation: Department of Thoracic and Cardiovascular Surgery, Yonsei University College of Medicine, Seoul, Korea.
authors.forename: Chang Young
authors.initials: CY
authors.lastname: Lee
authors.affiliation: Department of Thoracic and Cardiovascular Surgery, Yonsei University College of Medicine, Seoul, Korea.
authors.forename: Dae Joon
authors.initials: DJ
authors.lastname: Kim
authors.affiliation: Department of Thoracic and Cardiovascular Surgery, Yonsei University College of Medicine, Seoul, Korea.
authors.forename: Kyung Young
authors.initials: KY
authors.lastname: Chung
authors.affiliation: Department of Thoracic and Cardiovascular Surgery, Yonsei University College of Medicine, Seoul, Korea. csjglee@yuhs.ac.
authors.forename: Jin Gu
authors.initials: JG
authors.lastname: Lee
mesh_terms: D048868:Adaptor Proteins, Signal Transducing; D000368:Aged; D001343:Autophagy; D014408:Biomarkers, Tumor / Q000378:metabolism; D015153:Blotting, Western; D015179:Colorectal Neoplasms / Q000378:metabolism* / Q000473:pathology*; D005260:Female; D006801:Humans; D008175:Lung Neoplasms / Q000378:metabolism* / Q000473:pathology*; D008297:Male; D008840:Microfilament Proteins / Q000378:metabolism*; D046529:Microscopy, Electron, Transmission; D008869:Microtubule-Associated Proteins; D008875:Middle Aged; D009364:Neoplasm Recurrence, Local; D010865:Pilot Projects; D011506:Proteins; D012189:Retrospective Studies; D000071456:Sequestosome-1 Protein; D017211:Treatment Failure
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D014408:Biomarkers, Tumor; C502070:MAP1LC3B protein, human; D008840:Microfilament Proteins; D008869:Microtubule-Associated Proteins; D011506:Proteins; C099718:SQSTM1 protein, human; D000071456:Sequestosome-1 Protein
keywords: Colorectal cancer; autophagy; metastasis; treatment failure