title: Effects of glucose and glutamine concentrations in human dental pulp stem cells viability. An approach for cell transplantation.
abstract: OBJECTIVE
  To evaluate microscopic behavior and viability of dental pulp stem cells under glucose and glutamine deprivation.
  
  
  METHODS
  Human tooth tissues were minced in isolated pieces and cultured until the desired cellular proliferation for experimental phases. Cells were cultured under variations of glucose and glutamine in both serum presence and absence, and then those cells were evaluated according to number and viability by MTT assay. The confocal microscopy analyzed cytoskeleton, nucleus, and mitochondria integrity.
  
  
  RESULTS
  A low concentration of glucose favored cellular viability and microscopic behavior; the presence of glutamine in culture medium was favorable only when associated with glucose. The cellular biological potential in culture could be preserved in serum absence if nutritional requirements are adequate.
  
  
  CONCLUSIONS
  Cell microscopic behavior and viability have demonstrated better patterns on serum-free low glucose culture medium with glutamine deprivation.
authors.affiliation: Paulista School of Medicine, Federal University of Sao Paulo, Brazil.
authors.forename: Natasha
authors.initials: N
authors.lastname: Machado
authors.affiliation: Science and Technology Institute, Sao Jose dos Campos, SP, Brazil.
authors.forename: Silvio Eduardo
authors.initials: SE
authors.lastname: Duailibi
authors.affiliation: EPM, UNIFESP, Sao Paulo, SP, Brazil.
authors.forename: Jennifer Adriane dos
authors.initials: JA
authors.lastname: Santos
authors.affiliation: EPM, UNIFESP, Sao Paulo, SP, Brazil.
authors.forename: Vanessa
authors.initials: V
authors.lastname: Penna
authors.affiliation: Department of Surgery, UNIFESP, Sao Paulo, SP, Brazil.
authors.forename: Lydia Masako
authors.initials: LM
authors.lastname: Ferreira
authors.affiliation: Surgery Department, EPM, UNIFESP, Sao Paulo, SP, Brazil.
authors.forename: Monica Talarico
authors.initials: MT
authors.lastname: Duailibi
mesh_terms: D000293:Adolescent; D000328:Adult; D000704:Analysis of Variance; D002452:Cell Count; D018929:Cell Culture Techniques; D049109:Cell Proliferation; D002470:Cell Survival; D002478:Cells, Cultured; D003470:Culture Media; D003782:Dental Pulp / Q000502:physiology*; D005947:Glucose / Q000032:analysis*; D005973:Glutamine / Q000032:analysis*; D006801:Humans; D018613:Microscopy, Confocal; D033581:Stem Cell Transplantation / Q000379:methods*; D013234:Stem Cells / Q000502:physiology*; D055815:Young Adult
chemical_list: D003470:Culture Media; D005973:Glutamine; D005947:Glucose