title: [Hyperlipidemia inhibited inflammatory response to Porphyromonas gingivalis in apolipoprotein E knockout mice].
abstract: OBJECTIVE
  To investigate the effect and mechanism of hyperlipidemia on inflammatory response to Porphyromonas gingivalis(P. gingivalis) in apolipoprotein E knock out(ApoE-/-) mice.
  
  
  METHODS
  Long term hyperlipidemia model was established in ApoE-/- mice, and viable P. gingivalis were injected into peritoneal cavity to make peritonitis model. Bacteria clearance capability was detected by serial dilution, production of pro-inflammatory cytokine interleukin-6 (IL-6) and monocyte chemotactic protein-1 (MCP-1) in serum was measured by ELISA, and IL-6 as well as MCP-1 transcription in peritoneal cells was determined by real time PCR. The data was analyzed with SPSS13.0 software package.
  
  
  RESULTS
  Hyperlipidemia disrupted P. gingivalis clearance capability in ApoE-/- mice, accompanied by inhibited IL-6 and MCP-1 transcription in peritoneal cells and decreased IL-6 and MCP-1 release into blood.
  
  
  CONCLUSIONS
  Hyperlipidemia disrupts inflammatory response to P. gingivalis, leading to decreased bacteria clearance, which may affect periodontitis progression.
authors.affiliation: School and Hospital of Stomatology, Fujian Medical University. Fuzhou 350002, Fujian Province;China.E-mail:leilangdental@163.com.
authors.forename: Lang
authors.initials: L
authors.lastname: Lei
authors.forename: Hou-xuan
authors.initials: HX
authors.lastname: Li
authors.forename: Mei-xiang
authors.initials: MX
authors.lastname: Huang
authors.forename: Shuai
authors.initials: S
authors.lastname: Chen
authors.forename: Fu-hua
authors.initials: FH
authors.lastname: Yan
mesh_terms: D000818:Animals; D001053:Apolipoproteins; D002478:Cells, Cultured; D018932:Chemokine CCL2; D006949:Hyperlipidemias; D051379:Mice; D018345:Mice, Knockout; D010518:Periodontitis; D016966:Porphyromonas gingivalis
chemical_list: D001053:Apolipoproteins; D018932:Chemokine CCL2