title: Effects of Polygonatum sibiricum rhizome ethanol extract in high-fat diet-fed mice.
abstract: BACKGROUND
  The rhizome of Polygonatum sibiricum Redoute (Liliaceae) has long been used to treat diabetes-associated complications. However, the pharmacological mechanism of P. sibiricum on metabolic disorders is not clear.
  
  
  OBJECTIVE
  This study investigates the effect of an ethanol extract of P. sibiricum rhizomes (designated ID1216) on obesity conditions including weight loss in high-fat (HF) diet-fed mice and explores the potential underlying mechanisms.
  
  
  METHODS
  To identify the metabolic impact of the P. sibiricum rhizome extract, HF diet-fed mice were administered ID1216 orally at doses of 250 and 1000 mg/kg/d for 10 weeks, and various factors related to metabolic syndrome were analyzed. We also examined the effects of ID1216 on the expression of genes involved in adipogenesis and lipolysis in 3T3-L1 cells, as well as genes associated with energy homeostasis in C2C12 myocytes.
  
  
  RESULTS
  ID1216 administration led to significant decreases in body weight gain (37.5%), lipid accumulation in adipose tissues (52.8%), and the levels of plasma triglycerides (26.4%) and free fatty acids (28.1%) at a dose of 250 mg/kg/d, compared with the vehicle-treated group, as well as improved insulin resistance. In addition, ID1216 was found to regulate the expression of genes related to adipogenesis and fatty acid oxidation in 3T3-L1 cells and enhance the expression of genes that modulate energy homeostasis in C2C12 myocytes.
  
  
  CONCLUSIONS
  ID1216 may be a promising therapeutic agent for improving obesity conditions through the sirtuin-1 and peroxisome proliferator-activated receptor γ coactivator-1α pathway.
authors.affiliation: Research Laboratories, ILDONG Pharmaceutical Co., Ltd , Gyeonggi-Do , Korea.
authors.forename: Jong-Hee
authors.initials: JH
authors.lastname: Ko
authors.forename: Hyuk-Sang
authors.initials: HS
authors.lastname: Kwon
authors.forename: Jong-Min
authors.initials: JM
authors.lastname: Yoon
authors.forename: Jong-Su
authors.initials: JS
authors.lastname: Yoo
authors.forename: Hyeon-Soo
authors.initials: HS
authors.lastname: Jang
authors.forename: Ji-Young
authors.initials: JY
authors.lastname: Kim
authors.forename: Seung-Woo
authors.initials: SW
authors.lastname: Yeon
authors.forename: Jae-Hoon
authors.initials: JH
authors.lastname: Kang
mesh_terms: D041721:3T3-L1 Cells; D000818:Animals; D019440:Anti-Obesity Agents / Q000302:isolation & purification / Q000627:therapeutic use*; D001835:Body Weight / Q000187:drug effects; D059305:Diet, High-Fat / Q000009:adverse effects*; D004734:Energy Metabolism / Q000187:drug effects; D057809:HEK293 Cells; D006801:Humans; D008066:Lipolysis / Q000187:drug effects; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009765:Obesity / Q000188:drug therapy* / Q000378:metabolism; D000071248:Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha; D010936:Plant Extracts / Q000302:isolation & purification / Q000627:therapeutic use*; D031384:Polygonatum / Q000737:chemistry*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D027343:Rhizome / Q000737:chemistry; D056564:Sirtuin 1 / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics
chemical_list: D019440:Anti-Obesity Agents; D000071248:Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha; D010936:Plant Extracts; C485290:Ppargc1a protein, mouse; D014157:Transcription Factors; C470545:Sirt1 protein, mouse; D056564:Sirtuin 1
keywords: Metabolic disorder; Obesity; SIRT1