title: The effect of stem cell from human exfoliated deciduous teeth on T lymphocyte proliferation.
abstract: BACKGROUND
  Mesenchymal stem cells (MSC), a specific type of adult tissue stem cell; have the immunosuppressive effects that make them valuable targets for regenerative medicine and treatment of many human illnesses. Hence, MSC have been the subject of numerous studies. The classical source of MSC is adult bone marrow (BM). Due to many shortcomings of harvesting MSC from BM, finding the alternative sources for MSC is an urgent. Stem cells from human exfoliated deciduous teeth (SHED) are relative new MSC populations that fulfill these criteria but their potential immunosuppressive effect has not been studied enough yet. Thus, in this work the effect of SHED on the proliferation of in vitro activated T lymphocytes were explored.
  
  
  METHODS
  In this study, both mitogen and alloantigen activated T cells were cultured in the presence of different numbers of SHED. In some co-cultures, activated T cells were in direct contact to MSCs and in other co-cultures; they were separated from SHED by a permeable membrane. In all co-cultures, the proliferation of T cells was measured by ELISA Bromodeoxyuridine proliferation assay.
  
  
  RESULTS
  In general, our results showed that SHED significantly suppress the proliferation of activated T cells in a dose-dependent manner. Moreover, the suppression was slightly stronger when MSCs were in physical contact to activated T cells.
  
  
  CONCLUSIONS
  This study showed that SHED likewise other MSC populations can suppress the activation of T lymphocytes, which can be used instead of BM derived MSCs in many investigational and clinical applications.
authors.affiliation: Department of Immunology, Medical School, Isfahan University of Medical Sciences, Isfahan, Iran.
authors.forename: Razieh
authors.initials: R
authors.lastname: Alipour
authors.affiliation: Department of Immunology, Medical School, Isfahan University of Medical Sciences, Isfahan, Iran.
authors.forename: Minoo
authors.initials: M
authors.lastname: Adib
authors.affiliation: Department of Anatomical Sciences and Molecular Biology, Medical School, Isfahan University of Medical Sciences, Isfahan, Iran.
authors.forename: Batool
authors.initials: B
authors.lastname: Hashemi-Beni
authors.affiliation: Department of Anatomical Sciences and Molecular Biology, Medical School, Isfahan University of Medical Sciences, Isfahan, Iran.
authors.forename: Farzaneh
authors.initials: F
authors.lastname: Sadeghi
keywords: Immunosuppression; T lymphocytes; mesenchymal stem cells