title: In vitro effects of prolonged exposure to quercetin and epigallocatechin gallate of the peripheral blood mononuclear cell membrane.
abstract: The study aimed to assess biophysical changes that take place in the peripheral blood mononuclear cell (PBMC) membranes when exposed in vitro to 10 μM quercetin or epigallocatechin gallate (EGCG) for 24 and 48 h. PBMCs isolated from hypercholesterolemia patients were compared to those from normocholesterolemia subjects. The membrane fluidity and transmembrane potential were evaluated and the results were correlated with biochemical parameters relevant to oxidative stress, assessed in the patients' plasma. The baseline value of PBMC membrane anisotropy for the hypercholesterolemia patients was lower than that of the control group. These results correlated with the plasma levels of advanced glycation end products, which were significantly higher in the hypercholesterolemia group, and the total plasma antioxidant status, which was significantly higher in normocholesterolemia subjects. In the case of normocholesterolemia cells in vitro, polyphenols induced a decrease in membrane anisotropy (7.25-11.88% at 24 h, 1.82-2.26% at 48 h) and a hyperpolarizing effect (8.30-8.90% at 24 h and 4.58-13.00% at 48 h). The same effect was induced in hypercholesterolemia cells, but only after 48 h exposure to the polyphenols: the decrease in membrane anisotropy was 5.70% for quercetin and 2.33% for EGCG. After 48 h of in vitro incubation with the polyphenols, PBMCs isolated from hypercholesterolemia patients exhibited the effects that had been registered in cells from normocholesterolemia subjects after 24 h exposure. These results outlined the beneficial action of the studied polyphenols, quercetin and EGCG, as dietary supplements in normocholesterolemia and hypercholesterolemia patients.
authors.affiliation: Faculty of Pharmacy, Carol Davila University of Medicine and Pharmacy, 6 Traian Vuia St., 020956, Bucharest, Romania.
authors.forename: Denisa
authors.initials: D
authors.lastname: Margina
authors.forename: Mihaela
authors.initials: M
authors.lastname: Ilie
authors.forename: Gina
authors.initials: G
authors.lastname: Manda
authors.forename: Ionela
authors.initials: I
authors.lastname: Neagoe
authors.forename: Rucsandra
authors.initials: R
authors.lastname: Danciulescu-Miulescu
authors.forename: Carmen Nicoleta
authors.initials: CN
authors.lastname: Purdel
authors.forename: Daniela
authors.initials: D
authors.lastname: Gradinaru
mesh_terms: D000975:Antioxidants / Q000494:pharmacology*; D016022:Case-Control Studies; D002392:Catechin / Q000031:analogs & derivatives* / Q000494:pharmacology; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism*; D002478:Cells, Cultured; D004353:Drug Evaluation, Preclinical; D005454:Fluorescence Polarization; D017127:Glycation End Products, Advanced / Q000097:blood; D006937:Hypercholesterolemia / Q000097:blood; D007963:Leukocytes, Mononuclear / Q000187:drug effects*; D008560:Membrane Fluidity / Q000187:drug effects; D008564:Membrane Potentials / Q000187:drug effects; D018384:Oxidative Stress; D011794:Quercetin / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; D017127:Glycation End Products, Advanced; D002392:Catechin; D011794:Quercetin; C045651:epigallocatechin gallate