title: Delivery of therapeutic peptides and proteins to the CNS.
abstract: Peptides and proteins have potent effects on the brain after their peripheral administration, suggesting that they may be good substrates for the development of CNS therapeutics. Major hurdles to such development include their relation to the blood-brain barrier (BBB) and poor pharmacokinetics. Some peptides cross the BBB by transendothelial diffusion and others cross in the blood-to-brain direction by saturable transporters. Some regulatory proteins are also transported across the BBB and antibodies can enter the CNS via the extracellular pathways. Glycoproteins and some antibody fragments can be taken up and cross the BBB by mechanisms related to adsorptive endocytosis/transcytosis. Many peptides and proteins are transported out of the CNS by saturable efflux systems and enzymatic activity in the blood, CNS, or BBB are substantial barriers to others. Both influx and efflux transporters are altered by various substances and in disease states. Strategies that manipulate these interactions between the BBB and peptides and proteins provide many opportunities for the development of therapeutics. Such strategies include increasing transendothelial diffusion of small peptides, upregulation of saturable influx transporters with allosteric regulators and other posttranslational means, use of vectors and other Trojan horse strategies, inhibition of efflux transporters including with antisense molecules, and improvement in pharmacokinetic parameters to overcome short half-lives, tissue sequestration, and enzymatic degradation.
authors.affiliation: Geriatric Research Educational and Clinical Center, Veterans Affairs Puget Sound Health Care System, University of Washington, Seattle, Washington USA; Department of Medicine, Division of Gerontology and Geriatric Medicine, University of Washington, Seattle, Washington, USA.
authors.forename: Therese S
authors.initials: TS
authors.lastname: Salameh
authors.affiliation: Geriatric Research Educational and Clinical Center, Veterans Affairs Puget Sound Health Care System, University of Washington, Seattle, Washington USA; Department of Medicine, Division of Gerontology and Geriatric Medicine, University of Washington, Seattle, Washington, USA. Electronic address: wabanks1@uw.edu.
authors.forename: William A
authors.initials: WA
authors.lastname: Banks
mesh_terms: D000818:Animals; D001812:Blood-Brain Barrier / Q000378:metabolism*; D006801:Humans; D010455:Peptides / Q000008:administration & dosage / Q000493:pharmacokinetics* / Q000627:therapeutic use; D011506:Proteins / Q000008:administration & dosage / Q000493:pharmacokinetics* / Q000627:therapeutic use
chemical_list: D010455:Peptides; D011506:Proteins
keywords: Blood–brain barrier; Central nervous system; Drug delivery; Peptides; Proteins; Transport