title: Down-regulation of miR-140 induces EMT and promotes invasion by targeting Slug in esophageal cancer.
abstract: OBJECTIVE
  MicroRNAs (miRNAs) are reported to regulate cell invasion and functions by interfering with the translation of target mRNAs, but the role of miRNAs in esophageal cancer (EC) remains unclear.
  
  
  METHODS
  RT-PCR and Western blot were used to detect the expression of miRNAs and candidate genes in EC samples (n=89). miR-140 mimics and inhibitor were tansfected in human TE-1 and Eca-109 cells. The transwell assay was used to examine the cell invasive ability. The regulation mechanism was confirmed by luciferase reporter assay. The markers of EMT were detected by using Western blot.
  
  
  RESULTS
  miR-140 expression was decreased in the EC tissues compared with the corresponding adjacent tumor tissues. Low expression of miR-140 promotes cell invasion by using transwell assay, while the effect of miR-140 high expression is reverse. Slug, a target gene of miR-140, was examined by luciferase assay and Western blot.
  
  
  CONCLUSIONS
  miR-140 may regulate the cell invasion of EC via controlling Slug expression.
authors.affiliation: Department of Obstetrics and Gynecology, Affiliated Yixing Hospital of Jiangsu University, Yixing, People's Republic of China.
authors.forename: Weiling
authors.initials: W
authors.lastname: Li
authors.forename: Guojun
authors.initials: G
authors.lastname: Jiang
authors.forename: Jianping
authors.initials: J
authors.lastname: Zhou
authors.forename: Hongmin
authors.initials: H
authors.lastname: Wang
authors.forename: Zegang
authors.initials: Z
authors.lastname: Gong
authors.forename: Zheng
authors.initials: Z
authors.lastname: Zhang
authors.forename: Ke
authors.initials: K
authors.lastname: Min
authors.forename: Hongdi
authors.initials: H
authors.lastname: Zhu
authors.forename: Yongfei
authors.initials: Y
authors.lastname: Tan
mesh_terms: D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D015536:Down-Regulation / Q000235:genetics*; D004938:Esophageal Neoplasms / Q000235:genetics*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D009361:Neoplasm Invasiveness / Q000235:genetics*; D027701:Organic Cation Transport Proteins / Q000235:genetics*; D000071250:Snail Family Transcription Factors; D014157:Transcription Factors / Q000235:genetics*; D000099254:Organic Cation Transporter 3
chemical_list: D035683:MicroRNAs; D027701:Organic Cation Transport Proteins; D000071250:Snail Family Transcription Factors; D014157:Transcription Factors; D000099254:Organic Cation Transporter 3; C544302:Mirn140 microRNA, human; C000604407:SNAI1 protein, human