title: GABA and its B-receptor are present at the node of Ranvier in a small population of sensory fibers, implicating a role in myelination.
abstract: The γ-aminobutyric acid (GABA) type B receptor has been implicated in glial cell development in the peripheral nervous system (PNS), although the exact function of GABA signaling is not known. To investigate GABA and its B receptor in PNS development and degeneration, we studied the expression of the GABAB receptor, GABA, and glutamic acid decarboxylase GAD65/67 in both development and injury in fetal dissociated dorsal root ganglia (DRG) cell cultures and in the rat sciatic nerve. We found that GABA, GAD65/67, and the GABAB receptor were expressed in premyelinating and nonmyelinating Schwann cells throughout development and after injury. A small population of myelinated sensory fibers displayed all of these molecules at the node of Ranvier, indicating a role in axon-glia communication. Functional studies using GABAB receptor agonists and antagonists were performed in fetal DRG primary cultures to study the function of this receptor during development. The results show that GABA, via its B receptor, is involved in the myelination process but not in Schwann cell proliferation. The data from adult nerves suggest additional roles in axon-glia communication after injury.
authors.affiliation: Department of Neuroscience, Uppsala University, Uppsala, Sweden.
authors.forename: Mikael
authors.initials: M
authors.lastname: Corell
authors.forename: Grzegorz
authors.initials: G
authors.lastname: Wicher
authors.forename: Katarzyna J
authors.initials: KJ
authors.lastname: Radomska
authors.forename: E Duygu
authors.initials: ED
authors.lastname: Dağlıkoca
authors.forename: Randi Elberg
authors.initials: RE
authors.lastname: Godskesen
authors.forename: Robert
authors.initials: R
authors.lastname: Fredriksson
authors.forename: Eirikur
authors.initials: E
authors.lastname: Benedikz
authors.forename: Valerio
authors.initials: V
authors.lastname: Magnaghi
authors.forename: Asa
authors.initials: A
authors.lastname: Fex Svenningsen
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D049109:Cell Proliferation / Q000187:drug effects; D004622:Embryo, Mammalian; D018682:GABA Agents / Q000494:pharmacology; D005727:Ganglia, Spinal / Q000166:cytology; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D005904:Glial Fibrillary Acidic Protein / Q000235:genetics / Q000378:metabolism; D004676:Myelin Basic Protein / Q000235:genetics / Q000378:metabolism; D009185:Myelin Proteins / Q000235:genetics / Q000378:metabolism; D009186:Myelin Sheath / Q000378:metabolism*; D018992:Myelin-Associated Glycoprotein / Q000235:genetics / Q000378:metabolism; D009474:Neurons / Q000187:drug effects / Q000378:metabolism; D011901:Ranvier's Nodes / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D018080:Receptors, GABA-B / Q000235:genetics / Q000378:metabolism*; D012584:Sciatic Nerve / Q000166:cytology / Q000196:embryology / Q000254:growth & development; D020426:Sciatic Neuropathy / Q000378:metabolism / Q000473:pathology; D005680:gamma-Aminobutyric Acid / Q000378:metabolism*
chemical_list: D018682:GABA Agents; D005904:Glial Fibrillary Acidic Protein; C104739:Mag protein, mouse; D004676:Myelin Basic Protein; D009185:Myelin Proteins; D018992:Myelin-Associated Glycoprotein; C489545:Pmp22 protein, mouse; D018080:Receptors, GABA-B; D005680:gamma-Aminobutyric Acid
keywords: GABA; GABAB receptor; Schwann cells; nodes of Ranvier; peripheral nervous system