title: Nanostructured RNAs for RNA interference.
abstract: We synthesized three types of nanostructured RNAs that induce RNA interference (RNAi): branched RNAs, dumbbell-shaped RNA, and circular double-stranded RNAs. All three nanostructured RNAs were transformed into double-stranded RNA of approximately 20 base pairs when they were treated with nuclease enzymes such as Dicer. These dsRNA species induced gene silencing when they are were introduced into mammalian cells.
authors.affiliation: Nano Medical Engineering Laboratory, RIKEN, 2-1 Hirosawa, Wako, Saitama, 351-0198, Japan.
authors.forename: Yuko
authors.initials: Y
authors.lastname: Nakashima
authors.forename: Naoko
authors.initials: N
authors.lastname: Abe
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Ito
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Abe
mesh_terms: D060326:Chemistry Techniques, Synthetic; D053487:DEAD-box RNA Helicases / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D049329:Nanostructures / Q000737:chemistry*; D009690:Nucleic Acid Conformation; D009943:Organophosphorus Compounds / Q000737:chemistry; D034622:RNA Interference; D012322:RNA Precursors / Q000737:chemistry / Q000378:metabolism; D012330:RNA, Double-Stranded / Q000737:chemistry / Q000378:metabolism; D012333:RNA, Messenger / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism; D034741:RNA, Small Interfering / Q000138:chemical synthesis* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D043244:Ribonuclease III / Q000378:metabolism
chemical_list: D009943:Organophosphorus Compounds; D012322:RNA Precursors; D012330:RNA, Double-Stranded; D012333:RNA, Messenger; D034741:RNA, Small Interfering; C434331:phosphoramidite; C408087:DICER1 protein, human; D043244:Ribonuclease III; D053487:DEAD-box RNA Helicases