title: Dynamic proteomic profiles of in vivo- and in vitro-produced mouse postimplantation extraembryonic tissues and placentas.
abstract: As the interface between the mother and the developing fetus, the placenta is believed to play an important role in assisted reproductive technology (ART)-induced aberrant intrauterine and postnatal development. However, the mechanisms underlying aberrant placentation remain unclear, especially during extraembryonic tissue development and early stages of placental formation. Using a mouse model, this investigation provides the first comparative proteomic analysis of in vivo (IVO) and in vitro-produced (IVP) extraembryonic tissues and placentas after IVO fertilization and development, or in vitro fertilization and culture, respectively. We identified 165 and 178 differentially expressed proteins (DEPs) between IVO and IVP extraembryonic tissues and placentas on Embryonic Day 7.5 (E7.5) and E10.5, respectively. Many DEPs were functionally associated with genetic information processing, such as impaired de novo DNA methylation, as well as posttranscriptional, translational and posttranslational dysregulation. These novel findings were further confirmed by global hypomethylation, and a lower level of correlation was found between the transcriptome and proteome in the IVP groups. In addition, numerous DEPs were involved in energy and amino acid metabolism, cytoskeleton organization and transport, and vasculogenesis and angiogenesis. These disturbed processes and pathways are likely to be associated with embryonic intrauterine growth restriction, an enlarged placenta, and impaired labyrinth morphogenesis. This study provides a direct and comprehensive reference for the further exploration of the placental mechanisms that underlie ART-induced developmental aberrations.
authors.affiliation: Ministry of Agriculture Key Laboratory of Animal Genetics, Breeding and Reproduction, National Engineering Laboratory for Animal Breeding, College of Animal Sciences and Technology, China Agricultural University, Haidian, Beijing, China.
authors.forename: Linlin
authors.initials: L
authors.lastname: Sui
authors.affiliation: Ministry of Agriculture Key Laboratory of Animal Genetics, Breeding and Reproduction, National Engineering Laboratory for Animal Breeding, College of Animal Sciences and Technology, China Agricultural University, Haidian, Beijing, China.
authors.forename: Lei
authors.initials: L
authors.lastname: An
authors.affiliation: Ministry of Agriculture Key Laboratory of Animal Genetics, Breeding and Reproduction, National Engineering Laboratory for Animal Breeding, College of Animal Sciences and Technology, China Agricultural University, Haidian, Beijing, China.
authors.forename: Kun
authors.initials: K
authors.lastname: Tan
authors.affiliation: Ministry of Agriculture Key Laboratory of Animal Genetics, Breeding and Reproduction, National Engineering Laboratory for Animal Breeding, College of Animal Sciences and Technology, China Agricultural University, Haidian, Beijing, China.
authors.forename: Zhuqing
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Ministry of Agriculture Key Laboratory of Animal Genetics, Breeding and Reproduction, National Engineering Laboratory for Animal Breeding, College of Animal Sciences and Technology, China Agricultural University, Haidian, Beijing, China.
authors.forename: Shumin
authors.initials: S
authors.lastname: Wang
authors.affiliation: Ministry of Agriculture Key Laboratory of Animal Genetics, Breeding and Reproduction, National Engineering Laboratory for Animal Breeding, College of Animal Sciences and Technology, China Agricultural University, Haidian, Beijing, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Miao
authors.affiliation: Ministry of Agriculture Key Laboratory of Animal Genetics, Breeding and Reproduction, National Engineering Laboratory for Animal Breeding, College of Animal Sciences and Technology, China Agricultural University, Haidian, Beijing, China.
authors.forename: Likun
authors.initials: L
authors.lastname: Ren
authors.affiliation: Ministry of Agriculture Key Laboratory of Animal Genetics, Breeding and Reproduction, National Engineering Laboratory for Animal Breeding, College of Animal Sciences and Technology, China Agricultural University, Haidian, Beijing, China.
authors.forename: Li
authors.initials: L
authors.lastname: Tao
authors.affiliation: Ministry of Agriculture Key Laboratory of Animal Genetics, Breeding and Reproduction, National Engineering Laboratory for Animal Breeding, College of Animal Sciences and Technology, China Agricultural University, Haidian, Beijing, China.
authors.forename: Shuzhi
authors.initials: S
authors.lastname: He
authors.affiliation: Ministry of Agriculture Key Laboratory of Animal Genetics, Breeding and Reproduction, National Engineering Laboratory for Animal Breeding, College of Animal Sciences and Technology, China Agricultural University, Haidian, Beijing, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Yu
authors.affiliation: Ministry of Agriculture Key Laboratory of Animal Genetics, Breeding and Reproduction, National Engineering Laboratory for Animal Breeding, College of Animal Sciences and Technology, China Agricultural University, Haidian, Beijing, China.
authors.forename: Jinzhou
authors.initials: J
authors.lastname: Nie
authors.affiliation: BGI Tech Solutions Co., Ltd., Beishan Industrial Zone, Shenzhen, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Liu
authors.affiliation: BGI Tech Solutions Co., Ltd., Beishan Industrial Zone, Shenzhen, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Xing
authors.affiliation: Ministry of Agriculture Key Laboratory of Animal Genetics, Breeding and Reproduction, National Engineering Laboratory for Animal Breeding, College of Animal Sciences and Technology, China Agricultural University, Haidian, Beijing, China.
authors.forename: Zhonghong
authors.initials: Z
authors.lastname: Wu
authors.affiliation: Ministry of Agriculture Key Laboratory of Animal Genetics, Breeding and Reproduction, National Engineering Laboratory for Animal Breeding, College of Animal Sciences and Technology, China Agricultural University, Haidian, Beijing, China zchou@cau.edu.cn.
authors.forename: Zhuocheng
authors.initials: Z
authors.lastname: Hou
authors.affiliation: Ministry of Agriculture Key Laboratory of Animal Genetics, Breeding and Reproduction, National Engineering Laboratory for Animal Breeding, College of Animal Sciences and Technology, China Agricultural University, Haidian, Beijing, China tianjh@cau.edu.cn.
authors.forename: Jianhui
authors.initials: J
authors.lastname: Tian
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D046149:Embryo Culture Techniques; D004622:Embryo, Mammalian; D047108:Embryonic Development; D005321:Extraembryonic Membranes / Q000737:chemistry / Q000378:metabolism*; D005260:Female; D018507:Gene Expression Regulation, Developmental; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D010920:Placenta / Q000737:chemistry / Q000378:metabolism*; D011247:Pregnancy; D020543:Proteome / Q000032:analysis*; D040901:Proteomics
chemical_list: D020543:Proteome
keywords: assisted reproductive technology; extraembryonic tissues; in vitro fertilization; placenta; proteome