title: [Effects of early cigarette smoke exposure on the functions of T-lymphocyte subsets in asthmatic mice].
abstract: OBJECTIVE
  To explore the effects of early cigarette smoke exposure on the immune functions of T-lymphocyte subsets in asthmatic mice model.
  
  
  METHODS
  Forty mice (20 days) were randomly allocated into four groups: air control group, cigarette smoke (CS) control group, air+ovalbumin (OVA) group, and CS+OVA group (n = 10 each). The CS control and CS+OVA groups were exposed to cigarette smoke for 3 weeks while the other two groups exposed to air. At an age of 8 weeks, air+OVA and CS+OVA groups had OVA sensitization for establishing an asthma model while the other two groups were selected as control. The percentage of regulatory T cells (Treg), effect of T cell (Te) subgroup including T helper type (Th)1, Th2 and Th17 in splenic mononuclear T cell were determined by flow cytometry. The levels of transforming growth factor-β (TGF-β), interleukin (IL)-4, IL-5, IL-10, IL-13, IL-17A and interferon-γ (IFN-γ) in bronchoalveolar lavage fluid (BALF) were measured by enzyme-linked immunosorbent assay (ELISA). And the percentage ratios of Treg to Te were compared among four groups.
  
  
  RESULTS
  The percentages of Th1, Th2, Th17 and Treg in splenic mononuclear of CS+OVA group ((21.47 ± 2.84)%, (16.33 ± 3.02)%, (1.89 ± 0.23)% and (2.92 ± 0.40)%, respectively) were all significantly higher than those in air+OVA group ((14.78 ± 2.21)%, (9.72 ± 1.46)%, (1.01 ± 0.12)% and (1.84 ± 0.22)% , respectively), the levels of IL-4, IL-5, IL-10, IL-13, IL-17A, IFN- γ and TGF-β in BALF of CS+OVA group ((138.6 ± 18.9), (195.3 ± 32.6), (22.8 ± 3.9), (716.9 ± 123.6), (62.8 ± 7.9), (173.2 ± 25.5) and (66.5 ± 8.2) ng/L, respectively) were all significantly higher than those in air+OVA group ((90.1 ± 13.7), (128.5 ± 21.8), (14.4 ± 2.9), (421.4 ± 60.4), (40.4 ± 6.2), (113.8 ± 16.9) and (42.9 ± 3.8) ng/L, respectively) (all P < 0.05). No significant difference of Treg/Te percentage ratio existed in mice spleen mononuclear cells between air+OVA group and CS+OVA group (P > 0.05).
  
  
  CONCLUSIONS
  Early cigarette smoke exposure could significantly exacerbate T cell immune dysfunction in asthmatic mice.
authors.affiliation: Department of Pediatrics, First Hospital, Jilin University, Changchun 130021, China.
authors.forename: Yanan
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Pediatrics, First Hospital, Jilin University, Changchun 130021, China.
authors.forename: Li
authors.initials: L
authors.lastname: Liu
authors.affiliation: Department of Pediatrics, First Hospital, Jilin University, Changchun 130021, China.
authors.forename: Hongmei
authors.initials: H
authors.lastname: Qiao
authors.affiliation: Department of Pediatrics, First Hospital, Jilin University, Changchun 130021, China.
authors.forename: Hailong
authors.initials: H
authors.lastname: You
authors.affiliation: Department of Pediatrics, First Hospital, Jilin University, Changchun 130021, China. Email: hflyn2011@sina.com.
authors.forename: Huanji
authors.initials: H
authors.lastname: Cheng
mesh_terms: D000818:Animals; D001249:Asthma / Q000276:immunology*; D001992:Bronchoalveolar Lavage Fluid; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay; D007371:Interferon-gamma; D007378:Interleukins; D051379:Mice; D012907:Smoking / Q000009:adverse effects*; D016176:T-Lymphocyte Subsets / Q000276:immunology*; D014026:Nicotiana
chemical_list: D007378:Interleukins; D007371:Interferon-gamma