title: Alterations of mineral elements in osteoblast during differentiation under hypo, moderate and high static magnetic fields.
abstract: Static magnetic fields (SMFs) can enhance the ability of bone formation by osteoblast and is a potential physical therapy to bone disorders and the maintenance of bone health. But, the mechanism is not clear yet. Certain mineral elements including macro and trace elements are essential for normal bone metabolism. Deficiency of these elements can cause severe bone disorders including osteoporosis. However, there are few reports regarding the role of mineral elements in the regulation of bone formation under SMFs. In this study, hypomagnetic field (HyMF) of 500 nT, moderate SMF (MMF) of 0.2 T, and high SMF (HiMF) of 16 T were used to investigate the effects of SMFs on mineral element (calcium, copper, iron, magnesium, manganese, and zinc) alteration of MC3T3-E1 cells during osteoblast mineralization. The results showed that osteoblasts in differentiation accumulated more mineral elements than non-differentiated cell cultures. Furthermore, HyMF reduced osteoblast differentiation but did not affect mineral elements levels compared with control of geomagnetic field. MMF decreased osteoblast differentiation with elevated iron content. HiMF enhanced osteoblast differentiation and increased all the mineral contents except copper. It is suggested that the altered potential of osteoblast differentiation under SMFs may partially due to the involvement of different mineral elements.
authors.affiliation: Key Laboratory for Space Bioscience and Biotechnology, Institute of Special Environmental Biophysics, School of Life Sciences, Northwestern Polytechnical University, 127 Youyi Xilu, P.O. Box 707, Xi'an, Shaanxi, 710072, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Zhang
authors.forename: Chong
authors.initials: C
authors.lastname: Ding
authors.forename: Peng
authors.initials: P
authors.lastname: Shang
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000528:radiation effects; D002460:Cell Line; D060526:Magnetic Fields; D051379:Mice; D010006:Osteoblasts / Q000166:cytology* / Q000528:radiation effects*; D010012:Osteogenesis / Q000528:radiation effects