title: The expression and activity of antioxidant enzymes in the liver of rats exposed to high-fructose diet in the period from weaning to adulthood.
abstract: BACKGROUND
  Increased fructose consumption correlates with rising prevalence of various metabolic disorders, some of which were linked to oxidative stress. The relationship between fructose consumption and oxidative stress is complex and effects of a fructose-rich diet on the young population have not been fully elucidated. The aim of this study was to investigate whether high-fructose diet applied in the period from weaning to adulthood induces oxidative stress in the liver, thus contributing to induction or aggravation of metabolic disturbances in later adulthood. To that end we examined the effects of high-fructose diet on expression and activity of antioxidant enzymes, markers of lipid peroxidation and protein damage in the liver as the main fructose metabolizing tissue.
  
  
  RESULTS
  High-fructose diet increased only SOD2 (mitochondrial manganese superoxide dismutase) activity, with no effect on other antioxidant enzymes, lipid peroxidation or accumulation of damaged proteins in the liver.
  
  
  CONCLUSIONS
  The results show that fructose-induced metabolic disturbances could not be attributed to oxidative stress, at least not at young age. The absence of oxidative stress in the liver observed herein implies that young organisms are capable of maintaining redox homeostasis when challenged by fructose-derived energy overload.
authors.affiliation: Department of Biochemistry, Institute for Biological Research 'Siniša Stanković', University of Belgrade, 11060, Belgrade, Serbia.
authors.forename: Alhadi M
authors.initials: AM
authors.lastname: Glban
authors.affiliation: Department of Biochemistry, Institute for Biological Research 'Siniša Stanković', University of Belgrade, 11060, Belgrade, Serbia.
authors.forename: Ana
authors.initials: A
authors.lastname: Vasiljević
authors.affiliation: Department of Biochemistry, Institute for Biological Research 'Siniša Stanković', University of Belgrade, 11060, Belgrade, Serbia.
authors.forename: Nataša
authors.initials: N
authors.lastname: Veličković
authors.affiliation: Department of Physiology, Institute for Biological Research 'Siniša Stanković', University of Belgrade, 11060, Belgrade, Serbia.
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Nikolić-Kokić
authors.affiliation: Department of Physiology, Institute for Biological Research 'Siniša Stanković', University of Belgrade, 11060, Belgrade, Serbia.
authors.forename: Duško
authors.initials: D
authors.lastname: Blagojević
authors.affiliation: Department of Biochemistry, Institute for Biological Research 'Siniša Stanković', University of Belgrade, 11060, Belgrade, Serbia.
authors.forename: Gordana
authors.initials: G
authors.lastname: Matić
authors.affiliation: Department of Biochemistry, Institute for Biological Research 'Siniša Stanković', University of Belgrade, 11060, Belgrade, Serbia.
authors.forename: Jelena
authors.initials: J
authors.lastname: Nestorov
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D004032:Diet; D005632:Fructose / Q000009:adverse effects / Q000494:pharmacology*; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D008297:Male; D008659:Metabolic Diseases / Q000209:etiology; D018384:Oxidative Stress; D050050:Protein Carbonylation / Q000187:drug effects; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances; D014886:Weaning; D000099156:Superoxide Dismutase 2
chemical_list: D000975:Antioxidants; D005632:Fructose; D013482:Superoxide Dismutase; D017392:Thiobarbituric Acid Reactive Substances; D000099156:Superoxide Dismutase 2
keywords: antioxidant enzymes; fructose-fed rat; liver; oxidative stress; young rat