title: Combination of an unbiased amplification method and a resequencing microarray for detecting and genotyping equine arteritis virus.
abstract: This study shows that an unbiased amplification method applied to equine arteritis virus RNA significantly improves the sensitivity of the real-time reverse transcription-quantitative PCR (RT-qPCR) recommended by the World Organization for Animal Health. Twelve viral RNAs amplified using this method were hybridized on a high-density resequencing microarray for effective viral characterization.
authors.affiliation: ANSES-Dozulé Laboratory for Equine Diseases, Virology Unit, Goustranville, France aymeric.hans@anses.fr.
authors.forename: Aymeric
authors.initials: A
authors.lastname: Hans
authors.affiliation: ANSES-Dozulé Laboratory for Equine Diseases, Virology Unit, Goustranville, France.
authors.forename: Delphine
authors.initials: D
authors.lastname: Gaudaire
authors.affiliation: Institut Pasteur, Unité Environnement et Risques Infectieux, Cellule d'Intervention Biologique d'Urgence (CIBU), Paris, France.
authors.forename: Jean-Claude
authors.initials: JC
authors.lastname: Manuguerra
authors.affiliation: Frank Duncombe Laboratory, Animal Health Department, IFR146 ICORE, Caen, France.
authors.forename: Albertine
authors.initials: A
authors.lastname: Leon
authors.affiliation: Institut Pasteur, Unité d'Epidémiologie et Physiopathologie des Virus Oncogènes, Paris, France Centre National de la Recherche Scientifique, UMR 3569, Paris, France.
authors.forename: Antoine
authors.initials: A
authors.lastname: Gessain
authors.affiliation: ANSES-Dozulé Laboratory for Equine Diseases, Virology Unit, Goustranville, France.
authors.forename: Claire
authors.initials: C
authors.lastname: Laugier
authors.affiliation: Institut Pasteur, Unité d'Epidémiologie et Physiopathologie des Virus Oncogènes, Paris, France Centre National de la Recherche Scientifique, UMR 3569, Paris, France.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Berthet
authors.affiliation: Université Paris-Est, Anses Maisons-Alfort Laboratory for Animal Health, UMR1161 Virologie, Maisons-Alfort, France.
authors.forename: Stephan
authors.initials: S
authors.lastname: Zientara
mesh_terms: D000818:Animals; D018174:Arterivirus Infections / Q000175:diagnosis / Q000662:veterinary / Q000821:virology*; D007240:Equartevirus / Q000145:classification / Q000235:genetics; D060005:Genotyping Techniques / Q000379:methods*; D006734:Horse Diseases / Q000175:diagnosis / Q000821:virology*; D006736:Horses; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D010802:Phylogeny; D014773:Virology / Q000379:methods*