title: Isolation, cytotoxicity evaluation and HPLC-quantification of the chemical constituents from Prangos pabularia.
abstract: Phytochemical analysis of the dichloromethane:methanol (1:1) extract of root parts of Prangos pabularia led to the isolation of twelve cytotoxic constituents, viz., 6-hydroxycoumarin (1), 7-hydroxycoumarin (2), heraclenol-glycoside (3), xanthotoxol (4), heraclenol (5), oxypeucedanin hydrate (6), 8-((3,3-dimethyloxiran-2-yl)methyl)-7-methoxy-2H-chromen-2-one (7), oxypeucedanin hydrate monoacetate (8), xanthotoxin (9), 4-((2-hydroxy-3-methylbut-3-en-1-yl)oxy)-7H-furo[3,2-g]chromen-7-one (10), imperatorin (11) and osthol (12). The isolates were identified using spectral techniques in the light of literature. 3-(4,5-dimethyl thiazol-2yl)-2,5-diphenyltetrazolium bromide (MTT) cytotoxicity screening of the isolated constituents was carried out against six human cancer cell lines including lung (A549 and NCI-H322), epidermoid carcinoma (A431), melanoma (A375), prostate (PC-3) and Colon (HCT-116) cell lines. Osthol (12) exhibited the highest cytotoxicity with IC50 values of 3.2, 6.2, 10.9, 14.5, 24.8, and 30.2 µM against epidermoid carcinoma (A431), melanoma (A375), lung (NCI-H322), lung (A549), prostate (PC-3) and colon (HCT-116) cell lines respectively. Epidermoid carcinoma cell line A431 was sensitive to most of the compounds followed by lung (A549) cancer cell line. Finally a simple and reliable HPLC method was developed (RP-HPLC-DAD) and validated for the simultaneous quantification of these cytotoxic constituents in Prangos pabularia. The extract was analyzed using a reversed-phase Agilent ZORBAX eclipse plus column C18 (4.6×250 mm, 5 µm) at 250 nm wavelength using a gradient water-methanol solvent system at a flow rate of 0.8 ml/min. The RP-HPLC method is validated in terms of recovery, linearity, accuracy and precision (intra and inter-day validation). This method, because of shorter analysis time, makes it valuable for the commercial quality control of Prangos pabularia extracts and its future pharmaceutical preparations.
authors.affiliation: Bio-organic Chemistry Section, CSIR-Indian Institute of Integrative Medicine, Jammu, India.
authors.forename: Saleem
authors.initials: S
authors.lastname: Farooq
authors.affiliation: Bio-organic Chemistry Section, CSIR-Indian Institute of Integrative Medicine, Srinagar, India; Department of Chemistry, University of Kashmir, Srinagar, India.
authors.lastname: Shakeel-u-Rehman
authors.affiliation: Bio-organic Chemistry Section, CSIR-Indian Institute of Integrative Medicine, Jammu, India.
authors.forename: Nisar Ahmad
authors.initials: NA
authors.lastname: Dangroo
authors.affiliation: Cancer Pharmacology Division, CSIR-Indian Institute of Integrative Medicine, Jammu, India.
authors.forename: Dev
authors.initials: D
authors.lastname: Priya
authors.affiliation: Department of Chemistry, University of Kashmir, Srinagar, India.
authors.forename: Javid Ahmad
authors.initials: JA
authors.lastname: Banday
authors.affiliation: Bio-organic Chemistry Section, CSIR-Indian Institute of Integrative Medicine, Jammu, India.
authors.forename: Pyare Lal
authors.initials: PL
authors.lastname: Sangwan
authors.affiliation: Department of Chemistry, University of Kashmir, Srinagar, India.
authors.forename: Mushtaq Ahmad
authors.initials: MA
authors.lastname: Qurishi
authors.affiliation: Bio-organic Chemistry Section, CSIR-Indian Institute of Integrative Medicine, Jammu, India.
authors.forename: Surrinder
authors.initials: S
authors.lastname: Koul
authors.affiliation: Cancer Pharmacology Division, CSIR-Indian Institute of Integrative Medicine, Jammu, India.
authors.forename: Ajit Kumar
authors.initials: AK
authors.lastname: Saxena
mesh_terms: D019661:Apiaceae / Q000737:chemistry* / Q000378:metabolism; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D056148:Chromatography, Reverse-Phase; D004354:Drug Screening Assays, Antitumor; D045325:HCT116 Cells; D006801:Humans; D064209:Phytochemicals / Q000737:chemistry* / Q000302:isolation & purification / Q000633:toxicity*; D010936:Plant Extracts / Q000737:chemistry* / Q000302:isolation & purification / Q000633:toxicity*; D018517:Plant Roots / Q000737:chemistry / Q000378:metabolism
chemical_list: D064209:Phytochemicals; D010936:Plant Extracts