title: Effects of yerba maté, a plant extract formulation ("YGD") and resveratrol in 3T3-L1 adipogenesis.
abstract: We aimed to evaluate the in vitro effects of yerba maté, YGD (a herbal preparation containing yerba maté, guarana and damiana), and resveratrol on adipogenesis. The anti-adipogenic effects of yerba mate, YGD, resveratrol and YGD + resveratrol and yerba mate + resveratrol combinations were evaluated in 3T3-L1 cells by Oil Red staining, cellular triglyceride content, and PCR quantitative array. The results demonstrated that all of the tested compounds inhibited adipogenesis. Yerba maté extract significantly down-regulated the expression of genes that play an important role in regulating adipogenesis, such as Adig, Axin, Cebpa, Fgf10, Lep, Lpl, and Pparγ2. In addition, these genes, YGD also repressed Bmp2, Ccnd1, Fasn, and Srebf1. Resveratrol also modulated the expression of Adig, Bmp2, Ccnd1, C/EBPα, Fasn, Fgf10, Lep, Lpl, and Pparγ2. Moreover, resveratrol repressed Cebpb, Cdk4, Fgf2, and Klf15. The yerba maté extract and YGD up-regulated the expression of genes involved in inhibiting adipogenesis, such as Dlk-1, Klf2, and Ucp1. Resveratrol also induced the expression of Klf2 and Ucp1. In addition resveratrol modulated the Ddit3, Foxo1, Sirt1, and Sirt2. The combined effects of these compounds on gene expression showed similar results observed from individual treatments. Our data indicates that the synergy between the compounds favors the inhibition of adipogenesis.
authors.affiliation: Laboratory of Microbiology and Molecular Biology, Clinical Pharmacology and Gastroenterology Unit, Sao Francisco University Medical School, Bragança Paulista-SP 12916-900, Brazil. ju_carvalho_s@hotmail.com.
authors.forename: Juliana C
authors.initials: JC
authors.lastname: Santos
authors.affiliation: Laboratory of Microbiology and Molecular Biology, Clinical Pharmacology and Gastroenterology Unit, Sao Francisco University Medical School, Bragança Paulista-SP 12916-900, Brazil. erikinha_gotardo@yahoo.com.br.
authors.forename: Erica M F
authors.initials: EM
authors.lastname: Gotardo
authors.affiliation: Laboratory of Microbiology and Molecular Biology, Clinical Pharmacology and Gastroenterology Unit, Sao Francisco University Medical School, Bragança Paulista-SP 12916-900, Brazil. mitsue.brianti@gmail.com.
authors.forename: Mitsue T
authors.initials: MT
authors.lastname: Brianti
authors.affiliation: Groupe Persavita Inc., CP 49586 DU-MUSEE, Montreal, QC H3T 2A5, Canada. mpiraee@persavita.com.
authors.forename: Mahmood
authors.initials: M
authors.lastname: Piraee
authors.affiliation: Laboratory of Microbiology and Molecular Biology, Clinical Pharmacology and Gastroenterology Unit, Sao Francisco University Medical School, Bragança Paulista-SP 12916-900, Brazil. alessandra.gambero@usf.edu.br.
authors.forename: Alessandra
authors.initials: A
authors.lastname: Gambero
authors.affiliation: Laboratory of Microbiology and Molecular Biology, Clinical Pharmacology and Gastroenterology Unit, Sao Francisco University Medical School, Bragança Paulista-SP 12916-900, Brazil. marcelo.ribeiro@usf.edu.br.
authors.forename: Marcelo L
authors.initials: ML
authors.lastname: Ribeiro
mesh_terms: D041721:3T3-L1 Cells; D050156:Adipogenesis / Q000187:drug effects*; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism; D015153:Blotting, Western; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D004338:Drug Combinations; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000187:drug effects*; D030019:Ilex paraguariensis / Q000737:chemistry*; D051379:Mice; D009765:Obesity / Q000188:drug therapy* / Q000235:genetics / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D010936:Plant Extracts / Q000494:pharmacology*; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D000077185:Resveratrol; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013267:Stilbenes / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; D014408:Biomarkers, Tumor; D004338:Drug Combinations; D010936:Plant Extracts; D012333:RNA, Messenger; D013267:Stilbenes; D000077185:Resveratrol