title: GnRH analogue treatment on LH surge day 0 followed by single transvaginal artificial insemination with frozen semen on day 5 in bitches.
abstract: Reproductive parameters were evaluated in 19 and 14 estrous beagles that received 100 µg of gonadotropin-releasing hormone (GnRH) and saline treatment, respectively, on the day of luteinizing hormone (LH) surge (Day 0; estimated by serial progesterone assay) and balloon catheter-aided single transvaginal artificial insemination of frozen semen on Day 5. Although the conception rate and litter size were similar between the GnRH and saline groups, the concentration of LH peak was significantly higher in GnRH-treated bitches (P<0.01). In addition, the actual LH surge did not occur on the estimated Day 0 in one saline-treated bitch. In clinical practice that daily progesterone assay is difficult, administration of GnRH on estimated Day 0 would be recommended to induce or enhance the LH surge for timely and successful insemination.
authors.affiliation: Laboratory of Theriogenology, College of Bioresource Sciences, Nihon University, 1866 Kameino, Fujisawa, Kanagawa 252-0880, Japan.
authors.forename: Tadatoshi
authors.initials: T
authors.lastname: Ohtaki
authors.forename: Yasuna
authors.initials: Y
authors.lastname: Koga
authors.forename: Mamiko
authors.initials: M
authors.lastname: Ono
authors.forename: Gen
authors.initials: G
authors.lastname: Watanabe
authors.forename: Kazuyoshi
authors.initials: K
authors.lastname: Taya
authors.forename: Shigehisa
authors.initials: S
authors.lastname: Tsumagari
mesh_terms: D000818:Animals; D004285:Dogs / Q000502:physiology*; D005260:Female; D007987:Gonadotropin-Releasing Hormone / Q000008:administration & dosage / Q000494:pharmacology*; D007315:Insemination, Artificial / Q000662:veterinary*; D007986:Luteinizing Hormone / Q000097:blood / Q000378:metabolism*; D008297:Male; D011247:Pregnancy; D011374:Progesterone / Q000097:blood; D012662:Semen Preservation / Q000662:veterinary; D013081:Sperm Motility; D013094:Spermatozoa / Q000502:physiology
chemical_list: D007987:Gonadotropin-Releasing Hormone; D011374:Progesterone; D007986:Luteinizing Hormone