title: [Expression of SLC25A38 in leukemic cells from children with acute lymphoblastic leukemia].
abstract: This study was aimed to investigate the SLC25A38 expression in pediatric patients with acute lymphoblastic leukemia (ALL) and its clinical significance. A total of 23 newly diagnosed ALL pedictric patients were enrolled in test group, 10 pediatric patients with non-hematologic malignancies were selected as control group. The expression in protein and mRNA levels of SLC25A38 were detected by Western blot and real-time PCR respectively. The results showed that the SLC25A38 protein was positive in 8 of 23 pediatric ALL patients (34.78%), while no positive case was found in 10 controls. The relative expression level of SLC25A38 mRNA was 0.4673 ± 0.05344 in SLC25A38-protein positive group of ALL patients, while that was 1.296 ± 0.2517 in SLC25A38-protein negative group of ALL patients. The expression level of SLC25A38 mRNA in SLC25A38-protein positive group was significantly lower than that in negative group (P = 0.001) . No statistically significant difference was found in comparison of SLC25A38-protein negative group of ALL patients with the control group (P = 0.1097). The analysis of clinical data showed that there were significantly differences in sex, immunophenotype, initial peripheral white blood cell count and LDH between the SLC25A38-protein positive and SLC25A38-protein negative groups (P < 0.05). It is concluded that as a novel protein, SLC25A38 highly expressed in pediatric ALL patients, indicating that SLC25A38 may serve as a molecular marker and potential therapeutic target for acute lymphoblastic leukemia in children.
authors.affiliation: Department of Hematology, Zhongshan Hospital, Xiamen University, Xiamen 361004, Fujian Province, China.
authors.forename: Hua-Ying
authors.initials: HY
authors.lastname: Chen
authors.affiliation: Department of Hematology, Zhongshan Hospital, Xiamen University, Xiamen 361004, Fujian Province, China.
authors.forename: Yan-Yan
authors.initials: YY
authors.lastname: Lu
authors.affiliation: Department of Pediatrics, The First Affiliated Hospital of Xiamen University, Xiamen 361003, Fujian Province, China. E-mail: wenhong711@aliyun.com.
authors.forename: Hong
authors.initials: H
authors.lastname: Wen
authors.affiliation: Department of Hematology, Zhongshan Hospital, Xiamen University, Xiamen 361004, Fujian Province, China. E-mail: quanyilu@hotmail.com.
authors.forename: Quan-Yi
authors.initials: QY
authors.lastname: Lu
authors.affiliation: Institute of Neuroscienc, Xiamen University,Xiamen 361005, Fujian Province, China.
authors.forename: Yun-Wu
authors.initials: YW
authors.lastname: Zhang
authors.affiliation: Institute of Neuroscienc, Xiamen University, Xiamen 361005, Fujian Province, China.
authors.forename: Hua-Xi
authors.initials: HX
authors.lastname: Xu
mesh_terms: D002648:Child; D006801:Humans; D016130:Immunophenotyping; D007958:Leukocyte Count; D033681:Mitochondrial Membrane Transport Proteins / Q000378:metabolism*; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000378:metabolism*; D012333:RNA, Messenger; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D033681:Mitochondrial Membrane Transport Proteins; D012333:RNA, Messenger; C553226:Slc25a38 protein, human