title: [Effects of bortezomib combined with 5-azacytidine on the apoptosis of K562 cells and expression of SHIP mRNA].
abstract: This study was aimed to investigate the effects of bortezomib combined with 5-azacytidine on the apoptosis of K562 cells and expressiom of SHIP mRNA. The K562 cells were cultured and treated with different concentrations of bortezomib, 5-azacytidine or their combination for 24 hours. Then, the expression of SHIP mRNA was detected by RT-PCR,the cell proliferation was analyzed by using MTT assay and flow cytometry. The results showed that 5-20 nmol/L bortezomib could effectively inhibit the proliferation of K562 and this inhibitory effect gradually enhanced along with the increase of bortezomib concentration, the group of bortezomib combined with 5-azacytidine showed more inhibitory effect on K562 cells than that of bortezomib or 5-azacytidine alone.The bortezomib could promote the apoptosis of K562 cells in a dose-dependent manner,and this apoptotic effect was higher in group of bortezomib combined with 5-azacytidine than that in group of bortezomib or 5-azacytidine alone.Bortezomib could down-regulated the expression of SHIP mRNA in a dose-dependent manner,and this down-requlated effect was higher in group of bortezomib combined with 5-azacytidine than that in group of bortezomib or 5-azacytidine alone.It is concluded that bortezomib and 5-azacytidine can induce apoptosis by inhibiting the expression of SHIP mRNA in K562 cells.The combination of bortezomib with 5-azacytidine displays a synergetic effect.
authors.affiliation: Department of Hemotology,Baoding Municipal First Hospital, Baoding 071000, Hebei Province, China. E-mail: jzq980315@163.com.
authors.forename: Zhi-Qiang
authors.initials: ZQ
authors.lastname: Jia
authors.affiliation: Department of Hemotology,Baoding Municipal First Hospital, Baoding 071000, Hebei Province, China.
authors.forename: Yu-Tao
authors.initials: YT
authors.lastname: Wei
authors.affiliation: Sixth Department of Internal Medicine, Hebei Sixth People's Hospital, Baoding 071000, Hebei Province, China.
authors.forename: Yu-Lian
authors.initials: YL
authors.lastname: Wei
authors.affiliation: Department of Hemotology,Baoding Municipal First Hospital, Baoding 071000, Hebei Province, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Su
authors.affiliation: Department of Hemotology,Baoding Municipal First Hospital, Baoding 071000, Hebei Province, China.
authors.forename: Chun-Xia
authors.initials: CX
authors.lastname: Yu
authors.affiliation: Department of General Surgery, The Children's Hospital of Baoding City, Baoding 071000, Hebei Province, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Tao
authors.affiliation: Department of Hemotology,Baoding Municipal First Hospital, Baoding 071000, Hebei Province, China.
authors.forename: Hong-Qi
authors.initials: HQ
authors.lastname: Rong
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D001374:Azacitidine / Q000494:pharmacology*; D001897:Boronic Acids / Q000494:pharmacology*; D000069286:Bortezomib; D049109:Cell Proliferation; D006801:Humans; D000072181:Inositol Polyphosphate 5-Phosphatases; D020014:K562 Cells; D010744:Phosphoric Monoester Hydrolases / Q000235:genetics*; D011719:Pyrazines / Q000494:pharmacology*; D012333:RNA, Messenger / Q000096:biosynthesis*
chemical_list: D001897:Boronic Acids; D011719:Pyrazines; D012333:RNA, Messenger; D000069286:Bortezomib; D010744:Phosphoric Monoester Hydrolases; D000072181:Inositol Polyphosphate 5-Phosphatases; D001374:Azacitidine