title: Change in tear protein profile in diabetic retinopathy with duration of diabetes.
abstract: OBJECTIVE
  To study change in tear protein profile with duration of diabetes and severity of diabetic retinopathy (DR) in type 2 diabetes patients.
  
  
  METHODS
  Tear protein profile was ascertained by SDS PAGE method in 30 patients with DR (group A) and 37 patients without DR (group B).
  
  
  RESULTS
  Six distinct bands of proteins were identified; these proteins are as follows: 91kDa (P1), 66kDa (P2), 60kDa (P3), 30kDa (P4), 18.4kDa (P5) and 14.4kDa (P6). Prevalence of P3 was significant (p=0.036) in group A, especially in cases with diabetes ≤8 years compared with diabetes >8 years (p=0.0107). In group B, P2 was significantly prevalent (p<0.0013) in cases with diabetes ≤8 years compared to diabetes >8 years. Considering the changes in terms of duration of diabetes in general, patients with diabetes of ≤8 years, P3 was significantly prevalent in group A compared to group B (p=0.004); and when the duration of diabetes is >8 years, P2 was found significantly more in group A compared to group B (p=0.01). No significant difference in P3 (p=0.025), P4 (p=0.2877), P5 (p=0.4801), P6 (p=0.0985) was observed in mild to moderate NPDR group compared to severe NPDR to PDR group. P1 and P2 were present only in severe NPDR and PDR.
  
  
  CONCLUSIONS
  Variable protein expression was observed with duration of diabetes and severity of diabetic retinopathy.
authors.affiliation: Regional Institute of Ophthalmology, Medical College, Kolkata, Kolkata 700073, India. Electronic address: ghoshsrutarshi@gmail.com.
authors.forename: Srutarshi
authors.initials: S
authors.lastname: Ghosh
authors.affiliation: Regional Institute of Ophthalmology, Medical College, Kolkata, Kolkata 700073, India. Electronic address: sambuddhaghosh@gmail.com.
authors.forename: Sambuddha
authors.initials: S
authors.lastname: Ghosh
authors.affiliation: Department of Biochemistry, Ballygunj Science College, University of Calcutta, Kolkata 700019, India.
authors.forename: Mohammed
authors.initials: M
authors.lastname: Azharuddin
authors.affiliation: Regional Institute of Ophthalmology, Medical College, Kolkata, Kolkata 700073, India.
authors.forename: Sumanta
authors.initials: S
authors.lastname: Bera
authors.affiliation: Regional Institute of Ophthalmology, Medical College, Kolkata, Kolkata 700073, India.
authors.forename: Himadri
authors.initials: H
authors.lastname: Datta
authors.affiliation: Department of Biochemistry, Ballygunj Science College, University of Calcutta, Kolkata 700019, India.
authors.forename: Anjan
authors.initials: A
authors.lastname: Dasgupta
mesh_terms: D017668:Age of Onset; D001786:Blood Glucose / Q000378:metabolism; D018834:Chaperonin 60 / Q000378:metabolism; D003924:Diabetes Mellitus, Type 2 / Q000097:blood / Q000150:complications / Q000378:metabolism*; D003930:Diabetic Retinopathy / Q000378:metabolism*; D015352:Dry Eye Syndromes / Q000209:etiology / Q000378:metabolism*; D005136:Eye Proteins / Q000378:metabolism*; D005260:Female; D006442:Glycated Hemoglobin / Q000378:metabolism; D006801:Humans; D007194:India / Q000453:epidemiology; D008297:Male; D008875:Middle Aged; D024101:Mitochondrial Proteins / Q000378:metabolism; D015995:Prevalence; D012720:Severity of Illness Index; D011795:Surveys and Questionnaires; D013666:Tears / Q000378:metabolism*; D013997:Time Factors
chemical_list: D001786:Blood Glucose; D018834:Chaperonin 60; D005136:Eye Proteins; D006442:Glycated Hemoglobin; D024101:Mitochondrial Proteins; C060293:HSPD1 protein, human; C517652:hemoglobin A1c protein, human; C042434:tear proteins
keywords: Diabetes mellitus; Diabetic retinopathy; Protein; Tear