title: Tolerance and antioxidant response of a dark septate endophyte (DSE), Exophiala pisciphila, to cadmium stress.
abstract: The growth, oxidative damage and antioxidant response of Exophiala pisciphila ACCC32496, a dark septate endophyte isolated from an abandoned lead-zinc mining area, were measured at cadmium (Cd) concentrations of 0, 25, 50, 100, 200 and 400 mg L(-1). The EC50 values of E. pisciphila ACCC32496 to Cd were 332.2 mg L(-1) after 30 days on solid medium and 111.2 mg L(-1) after 7 days in liquid medium. Cd stress markedly stimulated the production of superoxide anion, H2O2 and malondialdehyde in the fungal mycelia. The activities of superoxide dismutase and catalase reached their maxima at 100 mg L(-1) Cd. The glutathione and non-protein thiol contents, along with the total antioxidant capability, reached their maxima at 50 mg L(-1) Cd. Low Cd concentrations induced a noticeable increase in antioxidant defense, while high Cd concentrations decreased the antioxidant defense.
authors.affiliation: College of Resources and Environment, Yunnan Agricultural University, Kunming, 650201, China.
authors.forename: Fangdong
authors.initials: F
authors.lastname: Zhan
authors.forename: Yongmei
authors.initials: Y
authors.lastname: He
authors.forename: Tao
authors.initials: T
authors.lastname: Li
authors.forename: Yun-ya
authors.initials: YY
authors.lastname: Yang
authors.forename: Gurpal S
authors.initials: GS
authors.lastname: Toor
authors.forename: Zhiwei
authors.initials: Z
authors.lastname: Zhao
mesh_terms: D000975:Antioxidants / Q000378:metabolism; D002104:Cadmium / Q000737:chemistry*; D002374:Catalase / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D060026:Endophytes / Q000187:drug effects* / Q000378:metabolism*; D004784:Environmental Monitoring / Q000379:methods; D005093:Exophiala / Q000378:metabolism*; D005978:Glutathione / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000378:metabolism; D008315:Malondialdehyde / Q000378:metabolism; D016877:Oxidants / Q000737:chemistry*; D010084:Oxidation-Reduction; D017382:Reactive Oxygen Species / Q000378:metabolism; D012989:Soil Pollutants / Q000737:chemistry; D013438:Sulfhydryl Compounds / Q000737:chemistry; D013482:Superoxide Dismutase / Q000378:metabolism; D013481:Superoxides / Q000378:metabolism
chemical_list: D000975:Antioxidants; D016877:Oxidants; D017382:Reactive Oxygen Species; D012989:Soil Pollutants; D013438:Sulfhydryl Compounds; D002104:Cadmium; D013481:Superoxides; D008315:Malondialdehyde; D006861:Hydrogen Peroxide; D002374:Catalase; D013482:Superoxide Dismutase; D005978:Glutathione