title: Quiescent neuronal progenitors are activated in the juvenile guinea pig lateral striatum and give rise to transient neurons.
abstract: In the adult brain, active stem cells are a subset of astrocytes residing in the subventricular zone (SVZ) and the dentate gyrus (DG) of the hippocampus. Whether quiescent neuronal progenitors occur in other brain regions is unclear. Here, we describe a novel neurogenic system in the external capsule and lateral striatum (EC-LS) of the juvenile guinea pig that is quiescent at birth but becomes active around weaning. Activation of neurogenesis in this region was accompanied by the emergence of a neurogenic-like niche in the ventral EC characterized by chains of neuroblasts, intermediate-like progenitors and glial cells expressing markers of immature astrocytes. Like neurogenic astrocytes of the SVZ and DG, these latter cells showed a slow rate of proliferation and retained BrdU labeling for up to 65 days, suggesting that they are the primary progenitors of the EC-LS neurogenic system. Injections of GFP-tagged lentiviral vectors into the SVZ and the EC-LS of newborn animals confirmed that new LS neuroblasts originate from the activation of local progenitors and further supported their astroglial nature. Newborn EC-LS neurons existed transiently and did not contribute to neuronal addition or replacement. Nevertheless, they expressed Sp8 and showed strong tropism for white matter tracts, wherein they acquired complex morphologies. For these reasons, we propose that EC-LS neuroblasts represent a novel striatal cell type, possibly related to those populations of transient interneurons that regulate the development of fiber tracts during embryonic life.
authors.affiliation: Department of Life Sciences and Systems Biology (DBIOS), University of Turin, Turin 10123, Italy Neuroscience Institute Cavalieri Ottolenghi (NICO), Orbassano 10010, Italy federico.luzzati@unito.it paolo.peretto@unito.it.
authors.forename: Federico
authors.initials: F
authors.lastname: Luzzati
authors.affiliation: Department of Life Sciences and Systems Biology (DBIOS), University of Turin, Turin 10123, Italy Neuroscience Institute Cavalieri Ottolenghi (NICO), Orbassano 10010, Italy.
authors.forename: Giulia
authors.initials: G
authors.lastname: Nato
authors.affiliation: Department of Life Sciences and Systems Biology (DBIOS), University of Turin, Turin 10123, Italy Neuroscience Institute Cavalieri Ottolenghi (NICO), Orbassano 10010, Italy.
authors.forename: Livio
authors.initials: L
authors.lastname: Oboti
authors.affiliation: Department of Oncology, University of Turin, c/o Institute for Cancer Research and Treatment (IRCC), Candiolo 10060, Italy.
authors.forename: Elisa
authors.initials: E
authors.lastname: Vigna
authors.affiliation: Neuroscience Institute Cavalieri Ottolenghi (NICO), Orbassano 10010, Italy Department of Neuroscience, University of Turin, Turin 10126, Italy.
authors.forename: Chiara
authors.initials: C
authors.lastname: Rolando
authors.affiliation: Neuroscience Institute Cavalieri Ottolenghi (NICO), Orbassano 10010, Italy Department of Veterinary Sciences, University of Turin, Grugliasco 10095, Italy.
authors.forename: Maria
authors.initials: M
authors.lastname: Armentano
authors.affiliation: Neuroscience Institute Cavalieri Ottolenghi (NICO), Orbassano 10010, Italy Department of Veterinary Sciences, University of Turin, Grugliasco 10095, Italy.
authors.forename: Luca
authors.initials: L
authors.lastname: Bonfanti
authors.affiliation: Department of Life Sciences and Systems Biology (DBIOS), University of Turin, Turin 10123, Italy Neuroscience Institute Cavalieri Ottolenghi (NICO), Orbassano 10010, Italy.
authors.forename: Aldo
authors.initials: A
authors.lastname: Fasolo
authors.affiliation: Department of Life Sciences and Systems Biology (DBIOS), University of Turin, Turin 10123, Italy Neuroscience Institute Cavalieri Ottolenghi (NICO), Orbassano 10010, Italy federico.luzzati@unito.it paolo.peretto@unito.it.
authors.forename: Paolo
authors.initials: P
authors.lastname: Peretto
mesh_terms: D000818:Animals; D005260:Female; D006168:Guinea Pigs; D008297:Male; D058953:Neural Stem Cells / Q000166:cytology* / Q000378:metabolism; D055495:Neurogenesis / Q000235:genetics / Q000502:physiology*; D009474:Neurons / Q000166:cytology* / Q000378:metabolism*; D046509:Tissue Culture Techniques
keywords: Internal capsule; Neural stem cells; Parenchymal progenitors; Postnatal neurogenesis; Striatum; Transient neurons