title: New Phenolics from Linum mucronatum subsp. orientale.
abstract: BACKGROUND
  Lignans and flavonoids are widely distributed phenolics in the plant kingdom. Aryltetralin type lignans (podophyllotoxin derivatives) as the major secondary metabolites of Linum species play an important role in the production of chemotherapy drugs. In the present study, lignans and flavonoid glycosides from aerial parts of Linum mucronatum subsp. orientale were isolated and identified.
  
  
  METHODS
  The phytochemical investigation has been carried out on Hexane, DCM and MeOH extracts of the plant. Separation of chemical constituents was done using different chromatography (CC, prep-TLC, GC/MS and HPLC) methods. The major compounds of dichloromethane (DCM) and methanol extracts were isolated and their structures were elucidated using co-chromatography in the presence of known lignans, HPLC and NMR techniques.
  
  
  RESULTS
  Our results showed that podophyllotoxin and 6-MeO-α- peltatin, as new compound, are the major lignans of the DCM extract of L. mucronatum subsp. orientale. Two new flavonoid glycosides were also elucidated in the methanolic extract.
  
  
  CONCLUSIONS
  The DCM and methanol extracts of L. mucronatum were found to contain aryltetralin-type lignans and flavonoids. The occurrence of 6-MeO-α- peltatin and flavonoids in L. mucronatum has been reported for the first time.
authors.affiliation: Department of Plant Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran ; Research Center for Pharmaceutical Nanotechnology, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran.
authors.forename: Khadijeh
authors.initials: K
authors.lastname: Zare
authors.affiliation: Department of Plant Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran.
authors.forename: Ali
authors.initials: A
authors.lastname: Movafeghi
authors.affiliation: Department of Agronomy and Plant Breeding, Faculty of Agriculture, University of Tabriz, Tabriz, Iran.
authors.forename: Sayed Abolghasem
authors.initials: SA
authors.lastname: Mohammadi
authors.affiliation: Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
authors.forename: Solmaz
authors.initials: S
authors.lastname: Asnaashari
authors.affiliation: Research Center for Pharmaceutical Nanotechnology, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran.
authors.forename: Hossein
authors.initials: H
authors.lastname: Nazemiyeh
keywords: 6-MeO-α- peltatin; Flavonoids; Lignan; Linum mucronatum; Mucronasides