title: Fine-tuned characterization of Staphylococcus aureus Newbould 305, a strain associated with mild and chronic mastitis in bovines.
abstract: S. aureus is a major aetiological agent of ruminant mastitis worldwide. The chronic nature of S. aureus mastitis makes it difficult to cure and prone to resurgence. In order to identify the bacterial factors involved in this chronicity, Newbould 305 (N305), a strain that can reproducibly induce mild and chronic mastitis in an experimental setting, was characterized in depth. We employed genomic and proteomic techniques combined with phenotype characterization, in order to comprehensively analyse N305. The results were compared with data obtained on S. aureus RF122, a strain representative of the major clone involved in severe bovine mastitis worldwide. Five mobile genetic elements were identified in the N305 genome as carrying virulence factors which correlated with phenotypic features such as cytotoxicity, mammary epithelial cell invasion or host-adaptation. In particular, the presence and characteristics of surface exposed proteins correlated well with the greater adhesion and internalization capacities of N305 in bovine mammary epithelial cells. N305 also displayed less diversity of toxin genes but secreted larger quantities of these toxins, associated with a higher cytotoxicity potential. Our data are consistent with the invasiveness and host-adaptation features which contribute to the chronicity of S. aureus mastitis. Mobile genetic elements, exoproteins and surface exposed proteins constitute good targets for further research to explore the underlying mechanisms related to mastitis chronicity.
authors.affiliation: INRA, UMR 1253 STLO, 65 rue de Saint Brieuc, 35042, Rennes Cedex, France. vincent.peton@rennes.inra.fr.|Agrocampus Ouest, UMR1253 STLO, 85 rue de Saint Brieuc, 35042, Rennes Cedex, France. vincent.peton@rennes.inra.fr.
authors.forename: Vincent
authors.initials: V
authors.lastname: Peton
authors.affiliation: INRA, UMR 1253 STLO, 65 rue de Saint Brieuc, 35042, Rennes Cedex, France. bouchard.dam@gmail.com.|Agrocampus Ouest, UMR1253 STLO, 85 rue de Saint Brieuc, 35042, Rennes Cedex, France. bouchard.dam@gmail.com.
authors.forename: Damien S
authors.initials: DS
authors.lastname: Bouchard
authors.affiliation: Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brasil. sintiaalmeida@yahoo.com.br.
authors.forename: Sintia
authors.initials: S
authors.lastname: Almeida
authors.affiliation: INRA, UMR 1253 STLO, 65 rue de Saint Brieuc, 35042, Rennes Cedex, France. lucie.rault@rennes.inra.fr.|Agrocampus Ouest, UMR1253 STLO, 85 rue de Saint Brieuc, 35042, Rennes Cedex, France. lucie.rault@rennes.inra.fr.
authors.forename: Lucie
authors.initials: L
authors.lastname: Rault
authors.affiliation: INRA, UMR 1253 STLO, 65 rue de Saint Brieuc, 35042, Rennes Cedex, France. helene.falentin@rennes.inra.fr.|Agrocampus Ouest, UMR1253 STLO, 85 rue de Saint Brieuc, 35042, Rennes Cedex, France. helene.falentin@rennes.inra.fr.
authors.forename: Hélène
authors.initials: H
authors.lastname: Falentin
authors.affiliation: INRA, UMR 1253 STLO, 65 rue de Saint Brieuc, 35042, Rennes Cedex, France. Julien.Jardin@rennes.inra.fr.|Agrocampus Ouest, UMR1253 STLO, 85 rue de Saint Brieuc, 35042, Rennes Cedex, France. Julien.Jardin@rennes.inra.fr.
authors.forename: Julien
authors.initials: J
authors.lastname: Jardin
authors.affiliation: INRA, UMR 1253 STLO, 65 rue de Saint Brieuc, 35042, Rennes Cedex, France. gwenael.jan@rennes.inra.fr.|Agrocampus Ouest, UMR1253 STLO, 85 rue de Saint Brieuc, 35042, Rennes Cedex, France. gwenael.jan@rennes.inra.fr.
authors.forename: Gwénaël
authors.initials: G
authors.lastname: Jan
authors.affiliation: Genomic Research Laboratory, Service of Infectious Diseases, University of Geneva Hospitals (HUG), CH-1211, Geneva 14, Switzerland. david.hernandez@genomic.ch.
authors.forename: David
authors.initials: D
authors.lastname: Hernandez
authors.affiliation: Genomic Research Laboratory, Service of Infectious Diseases, University of Geneva Hospitals (HUG), CH-1211, Geneva 14, Switzerland. patrice.francois@genomic.ch.
authors.forename: Patrice
authors.initials: P
authors.lastname: François
authors.affiliation: Genomic Research Laboratory, Service of Infectious Diseases, University of Geneva Hospitals (HUG), CH-1211, Geneva 14, Switzerland. Jacques.Schrenzel@hcuge.ch.
authors.forename: Jacques
authors.initials: J
authors.lastname: Schrenzel
authors.affiliation: Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brasil. vasco@icb.ufmg.br.
authors.forename: Vasco
authors.initials: V
authors.lastname: Azevedo
authors.affiliation: Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brasil. miyoshi@icb.ufmg.br.
authors.forename: Anderson
authors.initials: A
authors.lastname: Miyoshi
authors.affiliation: INRA, UMR 1253 STLO, 65 rue de Saint Brieuc, 35042, Rennes Cedex, France. nadia.berkova@rennes.inra.fr.|Agrocampus Ouest, UMR1253 STLO, 85 rue de Saint Brieuc, 35042, Rennes Cedex, France. nadia.berkova@rennes.inra.fr.
authors.forename: Nadia
authors.initials: N
authors.lastname: Berkova
authors.affiliation: INRA, UMR 1253 STLO, 65 rue de Saint Brieuc, 35042, Rennes Cedex, France. sergine.even@rennes.inra.fr.|Agrocampus Ouest, UMR1253 STLO, 85 rue de Saint Brieuc, 35042, Rennes Cedex, France. sergine.even@rennes.inra.fr.
authors.forename: Sergine
authors.initials: S
authors.lastname: Even
authors.affiliation: INRA, UMR 1253 STLO, 65 rue de Saint Brieuc, 35042, Rennes Cedex, France. yves.leloir@rennes.inra.fr.|Agrocampus Ouest, UMR1253 STLO, 85 rue de Saint Brieuc, 35042, Rennes Cedex, France. yves.leloir@rennes.inra.fr.
authors.forename: Yves
authors.initials: Y
authors.lastname: Le Loir
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D002417:Cattle; D002908:Chronic Disease; D005260:Female; D016680:Genome, Bacterial; D008414:Mastitis, Bovine / Q000382:microbiology*; D020543:Proteome; D013211:Staphylococcus aureus / Q000235:genetics / Q000502:physiology*; D037521:Virulence Factors / Q000235:genetics / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D020543:Proteome; D037521:Virulence Factors