title: Different types of cell cycle- and apoptosis-related gene expressions alter in corticosteroid-, vincristine-, and melphalan-resistant u-266 multiple myeloma cell lines.
abstract: OBJECTIVE
  Hemophilia B is caused by coagulation defects in the factor IX gene located in Xq27.1 on the X chromosome. A wide range of mutations, showing extensive molecular heterogeneity, have been described in hemophilia B patients. Our study was aimed at genetic analysis and prenatal diagnosis of hemophilia B in order to further elucidate the pathogenesis of the hemophilia B pedigree in China.
  
  
  METHODS
  Polymerase chain reaction amplification and direct sequencing of all the coding regions was conducted in hemophilia B patients and carriers. Prenatal diagnosis of the proband was conducted at 20 weeks.
  
  
  RESULTS
  We identified the novel point mutation 10.389 A>G, located upstream of the intron 3 acceptor site in hemophilia B patients. The fetus of the proband's cousin was identified as a carrier.
  
  
  CONCLUSIONS
  Our identification of a novel mutation in the F9 gene associated with hemophilia B provides novel insight into the pathogenesis of this genetically inherited disorder and also represents the basis of prenatal diagnosis.
authors.affiliation: Middle East Technical University, Department of Biological Sciences, Ankara, Turkey. E-ma-il: ufukg@metu.edu.tr.
authors.forename: Pelin
authors.initials: P
authors.lastname: Mutlu
authors.forename: Ali Uğur
authors.initials: AU
authors.lastname: Ural
authors.forename: Ufuk
authors.initials: U
authors.lastname: Gündüz