title: A variant of DC-SIGN gene promoter associated with resistance to HIV-1 in serodiscordant couples in Burkina Faso.
abstract: OBJECTIVE
  To study the involvement of variations in 4 genes associated with susceptibility and/or protection against HIV-1 in serodiscordant couples in Burkina Faso, namely, genes encoding HLA-B57, interferon regulatory factor 1 (IRF1), dendritic cell-specific ICAM3-grabbing nonintegrin (DC-SIGN) and CCR5 delta 32 (CCR5Δ32).
  
  
  METHODS
  Two DC-SIGN and two IRF1 single nucleotide polymorphisms (SNPs) as well as HLA-B57*01 and CCR5Δ32 alleles were genotyped in 51 serodiscordant couples in Burkina Faso. DC-SIGN, IRF1 and HLA-B57*01 genotyping was carried out by real time PCR using TaqMan assays (Applied Biosystems, USA and Sacace Biotechnologies, Italy). CCR5Δ32 deletion was investigated by PCR.
  
  
  RESULTS
  The two SNPs of DC-SIGN promoter showed a significant genotypic difference in serodiscordant couples. After multivariate analysis, only the association between DC-SIGN rs2287886 and HIV-1 remained significant (P<0.01). No association was found between IRF1 SNPs and HIV-1 infection. CCR5Δ32 wild type allele was found in 100% of serodiscordant couples. A high frequency of HLA-B57*01 allele was found in the HIV-positive (78%) compared with HIV-negative group (51%), however this difference was no longer significant after the correction of the sex confounding effect in the logistic regression model.
  
  
  CONCLUSIONS
  Our study suggests a protective role of a variation of DC-SIGN promoter and genetic resistance to HIV-1 in serodiscordant couples in Burkina Faso.
authors.affiliation: Biomolecular Research Center Pietro Annigoni CERBA/Labiogene University of Ouagadougou, Burkina Faso; Centre MURAZ Bobo-Dioulasso, Burkina Faso.
authors.forename: Thérèse Samdapawindé
authors.initials: TS
authors.lastname: Kagoné
authors.affiliation: Biomolecular Research Center Pietro Annigoni CERBA/Labiogene University of Ouagadougou, Burkina Faso; Laboratory of Molecular and Cellular Biology, University of Science and Technique of Masuku (USTM), Franceville, Gabon. Electronic address: cbisseye@gmail.com.
authors.forename: Cyrille
authors.initials: C
authors.lastname: Bisseye
authors.affiliation: Centre MURAZ Bobo-Dioulasso, Burkina Faso.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Méda
authors.affiliation: Centre MURAZ Bobo-Dioulasso, Burkina Faso.
authors.forename: Jean
authors.initials: J
authors.lastname: Testa
authors.affiliation: Health Centre Saint Camille of Nanoro, Burkina Faso.
authors.forename: Virginio
authors.initials: V
authors.lastname: Pietra
authors.affiliation: Centre MURAZ Bobo-Dioulasso, Burkina Faso.
authors.forename: Dramane
authors.initials: D
authors.lastname: Kania
authors.affiliation: Biomolecular Research Center Pietro Annigoni CERBA/Labiogene University of Ouagadougou, Burkina Faso.
authors.forename: Albert Théophane
authors.initials: AT
authors.lastname: Yonli
authors.affiliation: Biomolecular Research Center Pietro Annigoni CERBA/Labiogene University of Ouagadougou, Burkina Faso.
authors.forename: Tegwindé Rebeca
authors.initials: TR
authors.lastname: Compaoré
authors.affiliation: Biomolecular Research Center Pietro Annigoni CERBA/Labiogene University of Ouagadougou, Burkina Faso.
authors.forename: Jean Baptiste
authors.initials: JB
authors.lastname: Nikiéma
authors.affiliation: Faculty of Sciences, University of Lomé, Togo.
authors.forename: Comlan
authors.initials: C
authors.lastname: de Souza
authors.affiliation: Biomolecular Research Center Pietro Annigoni CERBA/Labiogene University of Ouagadougou, Burkina Faso.
authors.forename: Jacques
authors.initials: J
authors.lastname: Simpore
keywords: Burkina Faso; Genetic resistance; HIV-1