title: Assessment of ALA-induced PpIX production in porcine skin pretreated with microneedles.
abstract: Photodynamic therapy (PDT) is used for skin treatments of premalignant and cancer lesions and recognized as a non-invasive technique that combines tissue photosensitization and subsequent exposure to light to induce cell death. However, it is limited to the treatment of superficial lesions, mainly due to the low cream penetration. Therefore, the improvement of transdermal distribution of aminolevulinic acid (ALA) is needed. In this study, the kinetics and homogeneity of production of ALA-induced PpIX after the skin pre-treatment with microneedles rollers of 0.5, 1.0 and 1.5 mm length were investigated. An improvement in homogeneity and production of PpIX was shown in a porcine model. Widefield fluorescence imaging three hours after the topical application of ALA-cream in the combined treatment with microeedles rollers.
authors.affiliation: Sao Carlos Institute of Physics, University of Sao Paulo (USP), Biophotonics Laboratory, Sao Carlos-SP, Brazil. phamilla@ymail.com.
authors.forename: Phamilla Gracielli Sousa
authors.initials: PG
authors.lastname: Rodrigues
authors.affiliation: Sao Carlos Institute of Physics, University of Sao Paulo (USP), Biophotonics Laboratory, Sao Carlos-SP, Brazil.
authors.forename: Priscila Fernanda
authors.initials: PF
authors.lastname: Campos de Menezes
authors.affiliation: Sao Carlos Institute of Physics, University of Sao Paulo (USP), Biophotonics Laboratory, Sao Carlos-SP, Brazil.
authors.forename: Alessandra Keiko Lima
authors.initials: AK
authors.lastname: Fujita
authors.affiliation: Department of Clinical and Veterinary Surgery, São Paulo State University (UNESP), Jaboticabal-SP, Brazil.
authors.forename: André
authors.initials: A
authors.lastname: Escobar
authors.affiliation: Department of Clinical and Veterinary Surgery, São Paulo State University (UNESP), Jaboticabal-SP, Brazil.
authors.forename: Andrigo
authors.initials: A
authors.lastname: Barboza de Nardi
authors.affiliation: Sao Carlos Institute of Physics, University of Sao Paulo (USP), Biophotonics Laboratory, Sao Carlos-SP, Brazil.
authors.forename: Cristina
authors.initials: C
authors.lastname: Kurachi
authors.affiliation: Sao Carlos Institute of Physics, University of Sao Paulo (USP), Biophotonics Laboratory, Sao Carlos-SP, Brazil.
authors.forename: Vanderlei S
authors.initials: VS
authors.lastname: Bagnato
mesh_terms: D000622:Aminolevulinic Acid / Q000378:metabolism / Q000494:pharmacology*; D000818:Animals; D007700:Kinetics; D008297:Male; D009339:Needles; D011355:Prodrugs / Q000378:metabolism / Q000494:pharmacology; D011524:Protoporphyrins / Q000378:metabolism*; D012867:Skin / Q000187:drug effects* / Q000378:metabolism*; D034421:Sus scrofa
chemical_list: D011355:Prodrugs; D011524:Protoporphyrins; D000622:Aminolevulinic Acid; C028025:protoporphyrin IX
keywords: PpIX production; aminolevulinic acid; microneedle rollers; photodynamic therapy; porcine skin