title: Importance of NOS3 genetic polymorphisms in the risk of development of ischemic stroke in the Turkish population.
abstract: In the present study, we aimed to investigate the relationship between endothelial nitric oxide synthase 3 (NOS3) G894T, T-786C, and intron 4 variable number of tandem repeat (VNTR) variants, alone or in combination, and the risk of incidence of ischemic stroke in the Turkish population. The genotypes for all polymorphisms were determined by polymerase chain reaction/restriction fragment length polymorphism techniques on 245 ischemic stroke patients and 145 controls. In the case-control analysis, no significant difference was observed between stroke patients and controls with respect to NOS3 G894T, T-786C, and intron 4 VNTR polymorphisms genotype and allele frequency distribution. However, the copresence of G894T and intron 4 VNTR risk-elevating genotypes in the same individual increased the risk of stroke seven times (odds ratio=7.083, 95% confidence interval=0.866-57.963, p=0.029).
authors.affiliation: 1 Joint Graduate Program in Biochemistry, Department of Molecular Biology and Genetics, Middle East Technical University , Ankara, Turkey .
authors.forename: Aysun
authors.initials: A
authors.lastname: Türkanoğlu Özçelik
authors.forename: Birsen
authors.initials: B
authors.lastname: Can Demirdöğen
authors.forename: Şeref
authors.initials: Ş
authors.lastname: Demirkaya
authors.forename: Orhan
authors.initials: O
authors.lastname: Adalı
mesh_terms: D000368:Aged; D000483:Alleles; D002545:Brain Ischemia / Q000453:epidemiology / Q000235:genetics*; D016022:Case-Control Studies; D005260:Female; D005787:Gene Frequency; D006801:Humans; D015994:Incidence; D008297:Male; D008875:Middle Aged; D052250:Nitric Oxide Synthase Type III / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length; D012307:Risk Factors; D020521:Stroke / Q000453:epidemiology / Q000235:genetics*; D020080:Tandem Repeat Sequences; D014421:Turkey / Q000453:epidemiology
chemical_list: C496314:NOS3 protein, human; D052250:Nitric Oxide Synthase Type III