title: Synthesis, Characterization, and Biological Activity of N (')-[(Z)-(3-Methyl-5-oxo-1-phenyl-1,5-dihydro-4H-pyrazol-4-ylidene)(phenyl)methyl]benzohydrazide and Its Co(II), Ni(II), and Cu(II) Complexes.
abstract: Reaction of 1-phenyl-3-methyl-4-benzoyl-pyrazol-5-one and benzoyl hydrazide in refluxing ethanol gave N (')-[(Z)-(3-methyl-5-oxo-1-phenyl-1,5-dihydro-4H-pyrazol-4-ylidene)(phenyl)methyl]benzohydrazide (HL(1)), which was characterized by NMR spectroscopy and single-crystal X-ray structure study. X-ray diffraction analyses of the crystals revealed a nonplanar molecule, existing in the keto-amine form, with intermolecular hydrogen bonding forming a seven-membered ring system. The reaction of HL(1) with Co(II), Ni(II), and Cu(II) halides gave the corresponding complexes, which were characterized by elemental analysis, molar conductance, magnetic measurements, and infrared and electronic spectral studies. The compounds were screened for their in vitro cytotoxic activity against HL-60 human promyelocytic leukemia cells and antimicrobial activity against some bacteria and yeasts. Results showed that the compounds are potent against HL-60 cells with the IC50 value ≤5 μM, while some of the compounds were active against few studied Gram-positive bacteria.
authors.affiliation: Department of Pure and Industrial Chemistry, University of Nigeria, Enugu State, Nsukka 410001, Enugu State, Nigeria.
authors.forename: Jonnie N
authors.initials: JN
authors.lastname: Asegbeloyin
authors.affiliation: Department of Pure and Industrial Chemistry, University of Nigeria, Enugu State, Nsukka 410001, Enugu State, Nigeria.
authors.forename: Oguejiofo T
authors.initials: OT
authors.lastname: Ujam
authors.affiliation: Department of Pure and Industrial Chemistry, University of Nigeria, Enugu State, Nsukka 410001, Enugu State, Nigeria.
authors.forename: Emmanuel C
authors.initials: EC
authors.lastname: Okafor
authors.affiliation: Department of Chemistry, Adnan Menderes University, 09010 Aydin, Turkey.
authors.forename: Ilknur
authors.initials: I
authors.lastname: Babahan
authors.affiliation: Department of Biology, Adnan Menderes University, 09010 Aydin, Turkey.
authors.forename: Esin Poyrazoglu
authors.initials: EP
authors.lastname: Coban
authors.affiliation: Department of Biology, Adnan Menderes University, 09010 Aydin, Turkey.
authors.forename: Ali
authors.initials: A
authors.lastname: Ozmen
authors.affiliation: Department of Biology, Adnan Menderes University, 09010 Aydin, Turkey.
authors.forename: Halil
authors.initials: H
authors.lastname: Biyik