title: Diversity of glycosyl hydrolase enzymes from metagenome and their application in food industry.
abstract: Traditional use of enzymes for food processing and production of food ingredients resulted in fast-growing enzyme industries world over. The advances in technologies gave rise to exploring newer enzymes and/or modified enzymes for specific application. Search for novel enzymes that can augment catalytic efficiency and advances in molecular biology techniques including sequencing has targeted microbial diversity through metagenomic approaches for sourcing enzymes from difficult to culture organisms. Such mining studies have received more attention in characterizing hydrolases, their prevalence, broad substrate specificities, stability, and independence of cofactors. The focus on glycosyl hydrolases from metagenome for their application in food sector is reviewed.
authors.affiliation: Academy of Scientific and Innovative Research, New Delhi, India; CSIR-Central Food Technological Research Institute, Mysore-20, Karnataka, India.
authors.forename: T A
authors.initials: TA
authors.lastname: Sathya
authors.forename: Mahejibin
authors.initials: M
authors.lastname: Khan
mesh_terms: D002480:Cellulase / Q000235:genetics / Q000378:metabolism; D005511:Food Handling / Q000379:methods*; D006026:Glycoside Hydrolases / Q000235:genetics / Q000378:metabolism; D054892:Metagenome / Q000235:genetics*; D009699:N-Glycosyl Hydrolases / Q000235:genetics* / Q000378:metabolism; D011096:Polygalacturonase / Q000235:genetics / Q000378:metabolism; D013379:Substrate Specificity; D000516:alpha-Amylases / Q000235:genetics / Q000378:metabolism; D001616:beta-Galactosidase / Q000235:genetics / Q000378:metabolism
chemical_list: D006026:Glycoside Hydrolases; D000516:alpha-Amylases; D011096:Polygalacturonase; D001616:beta-Galactosidase; D002480:Cellulase; C019624:agarase; D009699:N-Glycosyl Hydrolases
keywords: functional food; glycosyl hydrolase; metagenomics; oligosaccharides