title: Sesamin attenuates neurotoxicity in mouse model of ischemic brain stroke.
abstract: Stroke is a severe neurological disorder characterized by the abrupt loss of blood circulation into the brain resulting into wide ranging brain and behavior abnormalities. The present study was designed to evaluate molecular mechanism by which sesamin (SES) induces neuroprotection in mouse model of ischemic stroke. The results of this study demonstrate that SES treatment (30 mg/kg bwt) significantly reduced infarction volume, lipid per-oxidation, cleaved-caspase-3 activation, and increased GSH activity following MCAO in adult male mouse. SES treatment also diminished iNOS and COX-2 protein expression, and significantly restored SOD activity and protein expression level in the ischemic cortex of the MCAO animals. Furthermore, SES treatment also significantly reduced inflammatory and oxidative stress markers including Iba1, Nox-2, Cox-2, peroxynitrite compared to placebo MCAO animals. Superoxide radical production, as studied by DHE staining method, was also significantly reduced in the ischemic cortex of SES treated compared to placebo MCAO animals. Likewise, downstream effects of superoxide free radicals i.e. MAPK/ERK and P38 activation was also significantly attenuated in SES treated compared to placebo MCAO animals. In conclusion, these results suggest that SES induces significant neuroprotection, by ameliorating many signaling pathways activated/deactivated following cerebral ischemia in adult mouse.
authors.affiliation: Department of Biological Sciences, Rabigh College of Science and Arts, King Abdulaziz University (Jeddah), P.O. Box 344, Rabigh 21911, Kingdom of Saudi Arabia. Electronic address: asaif77@yahoo.com.
authors.forename: Saif
authors.initials: S
authors.lastname: Ahmad
authors.affiliation: Department of Clinical Biochemistry, Mansoura University, Mansoura, Egypt.
authors.forename: Nehal M
authors.initials: NM
authors.lastname: Elsherbiny
authors.affiliation: Centre for Biological Science (Biotechnology), Central University of Bihar, Patna, Bihar, India.
authors.forename: Rizwanul
authors.initials: R
authors.lastname: Haque
authors.affiliation: Department of Neurology; Georgia Regents University, Augusta, GA, USA.
authors.forename: M Badruzzaman
authors.initials: MB
authors.lastname: Khan
authors.affiliation: Department of Clinical and Administrative Pharmacy, University of Georgia, Augusta, GA, USA.
authors.forename: Tauheed
authors.initials: T
authors.lastname: Ishrat
authors.affiliation: Department of Medicinal and Biological Chemistry, The University of Toledo, Toledo, OH, USA.
authors.forename: Zahoor A
authors.initials: ZA
authors.lastname: Shah
authors.affiliation: Department of Biochemistry, Faculty of Medicine, Zawia University, AZ-Zawia, Libya.
authors.forename: Mohammad M
authors.initials: MM
authors.lastname: Khan
authors.affiliation: The Research Institute at Nationwide Children's Hospital, Columbus, OH, USA.
authors.forename: Mehboob
authors.initials: M
authors.lastname: Ali
authors.affiliation: Department of Biological Sciences, Rabigh College of Science and Arts, King Abdulaziz University (Jeddah), P.O. Box 344, Rabigh 21911, Kingdom of Saudi Arabia.
authors.forename: Arshad
authors.initials: A
authors.lastname: Jamal
authors.affiliation: Department of Biotechnology, Amity University, Noida, UP, India.
authors.forename: Deepshikha Pande
authors.initials: DP
authors.lastname: Katare
authors.affiliation: Department of Ophthalmology, School of Medicine, Georgia Regents University, Augusta, GA, USA.
authors.forename: Gregory I
authors.initials: GI
authors.lastname: Liou
authors.affiliation: Department of Biological Sciences, Rabigh College of Science and Arts, King Abdulaziz University (Jeddah), P.O. Box 344, Rabigh 21911, Kingdom of Saudi Arabia. Electronic address: kanchan1980@gmail.com.
authors.forename: Kanchan
authors.initials: K
authors.lastname: Bhatia
mesh_terms: D000818:Animals; D002545:Brain Ischemia / Q000188:drug therapy* / Q000378:metabolism; D004149:Dioxoles / Q000627:therapeutic use*; D004195:Disease Models, Animal; D004660:Encephalitis / Q000188:drug therapy / Q000378:metabolism; D020244:Infarction, Middle Cerebral Artery; D017705:Lignans / Q000627:therapeutic use*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D017628:Microglia / Q000187:drug effects / Q000378:metabolism; D018696:Neuroprotective Agents / Q000627:therapeutic use*; D018384:Oxidative Stress / Q000187:drug effects; D015398:Signal Transduction / Q000187:drug effects*; D020521:Stroke / Q000188:drug therapy* / Q000378:metabolism
chemical_list: D004149:Dioxoles; D017705:Lignans; D018696:Neuroprotective Agents; C054125:sesamin
keywords: Antioxidants; Inflammation; MAPKinase ameliorate; Sesamin; Stroke