title: BRCA1 accelerates CtIP-mediated DNA-end resection.
abstract: DNA-end resection is a highly regulated and critical step in the response and repair of DNA double-strand breaks. In higher eukaryotes, CtIP regulates resection by integrating cellular signals via its posttranslational modifications and protein-protein interactions, including cell-cycle-controlled interaction with BRCA1. The role of BRCA1 in DNA-end resection is not clear. Here, we develop an assay to study DNA resection in higher eukaryotes at high resolution. We demonstrate that the BRCA1-CtIP interaction, albeit not essential for resection, modulates the speed at which this process takes place.
authors.affiliation: Centro Andaluz de Biología Molecular y Medicina Regenerativa (CABIMER), 41092 Sevilla, Spain; Departamento de Genética, Universidad de Sevilla, 41080, Sevilla, Spain.
authors.forename: Andrés
authors.initials: A
authors.lastname: Cruz-García
authors.affiliation: Centro Andaluz de Biología Molecular y Medicina Regenerativa (CABIMER), 41092 Sevilla, Spain; Departamento de Genética, Universidad de Sevilla, 41080, Sevilla, Spain.
authors.forename: Ana
authors.initials: A
authors.lastname: López-Saavedra
authors.affiliation: Centro Andaluz de Biología Molecular y Medicina Regenerativa (CABIMER), 41092 Sevilla, Spain; Departamento de Genética, Universidad de Sevilla, 41080, Sevilla, Spain. Electronic address: pablo.huertas@cabimer.es.
authors.forename: Pablo
authors.initials: P
authors.lastname: Huertas
mesh_terms: D019313:BRCA1 Protein / Q000235:genetics / Q000378:metabolism*; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D045744:Cell Line, Tumor; D004260:DNA Repair; D004706:Endodeoxyribonucleases; D057809:HEK293 Cells; D006801:Humans; D007700:Kinetics; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding
chemical_list: D019313:BRCA1 Protein; C492913:BRCA1 protein, human; D002352:Carrier Proteins; D009687:Nuclear Proteins; D004706:Endodeoxyribonucleases; C410741:RBBP8 protein, human