title: Dielectrophoresis for bioparticle manipulation.
abstract: As an ideal method to manipulate biological particles, the dielectrophoresis (DEP) technique has been widely used in clinical diagnosis, disease treatment, drug development, immunoassays, cell sorting, etc. This review summarizes the research in the field of bioparticle manipulation based on DEP techniques. Firstly, the basic principle of DEP and its classical theories are introduced in brief; Secondly, a detailed introduction on the DEP technique used for bioparticle manipulation is presented, in which the applications are classified into five fields: capturing bioparticles to specific regions, focusing bioparticles in the sample, characterizing biomolecular interaction and detecting microorganism, pairing cells for electrofusion and separating different kinds of bioparticles; Thirdly, the effect of DEP on bioparticle viability is analyzed; Finally, the DEP techniques are summarized and future trends in bioparticle manipulation are suggested.
authors.affiliation: Robotics and Microsystems Center, College of Mechanical and Electrical Engineering & Collaborative Innovation Center of Suzhou Nano Science and Technology, Soochow University, Suzhou 215000, China. ffsyccc@163.com.
authors.forename: Cheng
authors.initials: C
authors.lastname: Qian
authors.affiliation: Robotics and Microsystems Center, College of Mechanical and Electrical Engineering & Collaborative Innovation Center of Suzhou Nano Science and Technology, Soochow University, Suzhou 215000, China. hbhuang@suda.edu.cn.
authors.forename: Haibo
authors.initials: H
authors.lastname: Huang
authors.affiliation: Robotics and Microsystems Center, College of Mechanical and Electrical Engineering & Collaborative Innovation Center of Suzhou Nano Science and Technology, Soochow University, Suzhou 215000, China. chenliguo@suda.edu.cn.
authors.forename: Liguo
authors.initials: L
authors.lastname: Chen
authors.affiliation: Robotics and Microsystems Center, College of Mechanical and Electrical Engineering & Collaborative Innovation Center of Suzhou Nano Science and Technology, Soochow University, Suzhou 215000, China. licool@mail.ustc.edu.cn.
authors.forename: Xiangpeng
authors.initials: X
authors.lastname: Li
authors.affiliation: Robotics and Microsystems Center, College of Mechanical and Electrical Engineering & Collaborative Innovation Center of Suzhou Nano Science and Technology, Soochow University, Suzhou 215000, China. gezunbiao@hotmail.com.
authors.forename: Zunbiao
authors.initials: Z
authors.lastname: Ge
authors.affiliation: Robotics and Microsystems Center, College of Mechanical and Electrical Engineering & Collaborative Innovation Center of Suzhou Nano Science and Technology, Soochow University, Suzhou 215000, China. cht22@sina.com.
authors.forename: Tao
authors.initials: T
authors.lastname: Chen
authors.affiliation: Robotics and Microsystems Center, College of Mechanical and Electrical Engineering & Collaborative Innovation Center of Suzhou Nano Science and Technology, Soochow University, Suzhou 215000, China. yangzhan@suda.edu.cn.
authors.forename: Zhan
authors.initials: Z
authors.lastname: Yang
authors.affiliation: Robotics and Microsystems Center, College of Mechanical and Electrical Engineering & Collaborative Innovation Center of Suzhou Nano Science and Technology, Soochow University, Suzhou 215000, China. lnsun@hit.edu.cn.
authors.forename: Lining
authors.initials: L
authors.lastname: Sun
mesh_terms: D000818:Animals; D001419:Bacteria / Q000302:isolation & purification; D002469:Cell Separation / Q000295:instrumentation / Q000379:methods*; D004586:Electrophoresis / Q000295:instrumentation / Q000379:methods*; D004867:Equipment Design; D006801:Humans; D056656:Lab-On-A-Chip Devices; D046208:Microchip Analytical Procedures / Q000379:methods; D008846:Micromanipulation / Q000295:instrumentation / Q000379:methods*