title: Evaluation of qualitative and quantitative immunoassays to detect barley contamination in gluten-free beer with confirmation using LC/MS/MS.
abstract: To meet the need for the detection and quantitation of barley gluten in beer, qualitative screening and quantitative immunoassays based on the monoclonal antigluten antibody 401/21 (Skerritt) were validated in a single laboratory. Sample replicates were tested at each stage of beer production using multiple yeast strains and methods of end-stage protein removal. Quantitation was performed using barley-specific standards based on barley flour extracts. Immunoassay results were confirmed using LC/MS/MS for barley-specific peptides. The LOD for the qualitative screening test was 5 mg/L barley gluten. Recovery for the barley-spiked worts ranged from 81 to 128% in the quantitative ELISA assay; the LOD was <1 mg/L, and the LOQ was 5 mg/L. Both screening and confirmation methods were found to be fit for the purposes of detection of low levels of barley gluten in beer.
authors.forename: Laura K
authors.initials: LK
authors.lastname: Allred
authors.forename: Jennifer A
authors.initials: JA
authors.lastname: Sealey Voyksner
authors.forename: Robert D
authors.initials: RD
authors.lastname: Voyksner
mesh_terms: D000595:Amino Acid Sequence; D001515:Beer / Q000032:analysis*; D002853:Chromatography, Liquid; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005983:Glutens / Q000032:analysis*; D001467:Hordeum / Q000737:chemistry*; D057230:Limit of Detection; D008969:Molecular Sequence Data; D010455:Peptides / Q000032:analysis; D053719:Tandem Mass Spectrometry
chemical_list: D010455:Peptides; D005983:Glutens