title: Nosocomial dissemination of plasmids carrying blaTEM-24, blaDHA-1, aac(6')-Ib-cr, and qnrA6 in Providencia spp. strains isolated from a Tunisian hospital.
abstract: The aim of this study is to report the emergence of IncA/C conjugative plasmids harboring blaTEM-24, blaDHA-1, qnrA6, and aac(6')-Ib-cr genes among Providencia spp. isolates recovered in 2008 in Tunisia. The double-disk synergy test confirmed the phenotype extended-spectrum β-lactamase (ESBL) in 2 Providencia stuartii and 5 Providencia rettgeri. These ESBLs were coresistant to cefoxitin, chloramphenicol, ofloxacin, and sulfonamides but remained susceptible to imipenem. Three β-lactamases TEM-2, TEM-24, and DHA-1 were detected. blaTEM-24, blaDHA-1, aac(6')-Ib-cr, and qnrA6 genes were successfully transferred to Escherichia coli strain HB101, and they were found located on the conjugatifs IncA/C plasmids. Genetic relatedness showed similar and different patterns among P. stuartii and P. rettgeri strains, respectively.
authors.affiliation: Laboratory of Biochemistry and Biotechnology, Department of Biology, Faculty of Sciences of Tunis, Campus Universitaire, 2092 El-Manar II, Tunis, Tunisia. Electronic address: sihemmahrouki@yahoo.fr.
authors.forename: Sihem
authors.initials: S
authors.lastname: Mahrouki
authors.affiliation: Laboratory of Biochemistry and Biotechnology, Department of Biology, Faculty of Sciences of Tunis, Campus Universitaire, 2092 El-Manar II, Tunis, Tunisia.
authors.forename: Hela
authors.initials: H
authors.lastname: Chihi
authors.affiliation: Laboratory of Biochemistry and Biotechnology, Department of Biology, Faculty of Sciences of Tunis, Campus Universitaire, 2092 El-Manar II, Tunis, Tunisia.
authors.forename: Amel
authors.initials: A
authors.lastname: Bourouis
authors.affiliation: Laboratory of Biochemistry and Biotechnology, Department of Biology, Faculty of Sciences of Tunis, Campus Universitaire, 2092 El-Manar II, Tunis, Tunisia.
authors.forename: Khaoula
authors.initials: K
authors.lastname: Ayari
authors.affiliation: Intensive care Unit Ward, Military Hospital of Tunis, 1089 Monfleury, Tunis, Tunisia.
authors.forename: Mustapha
authors.initials: M
authors.lastname: Ferjani
authors.affiliation: Laboratory of microbiology, Department of Biology, Military Hospital of Tunis, 1089 Monfleury, Tunis, Tunisia.
authors.forename: Mohamed Ben
authors.initials: MB
authors.lastname: Moussa
authors.affiliation: Laboratory of Biochemistry and Biotechnology, Department of Biology, Faculty of Sciences of Tunis, Campus Universitaire, 2092 El-Manar II, Tunis, Tunisia.
authors.forename: Omrane
authors.initials: O
authors.lastname: Belhadj
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics; D003428:Cross Infection / Q000188:drug therapy / Q000382:microbiology*; D004756:Enterobacteriaceae Infections / Q000188:drug therapy / Q000382:microbiology*; D005798:Genes, Bacterial; D006761:Hospitals; D006801:Humans; D008826:Microbial Sensitivity Tests; D010957:Plasmids / Q000235:genetics; D011532:Providencia / Q000187:drug effects* / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity; D016896:Treatment Outcome; D014416:Tunisia; D001618:beta-Lactamases / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; C048795:AmpC beta-lactamases; D001618:beta-Lactamases; C056342:beta-lactamase TEM-2
keywords: AmpC β-lactamase; ESBL; IncA/C plasmid; Providencia; qnrA6