title: Balanced anesthesia with sevoflurane does not alter redox status in patients undergoing surgical procedures.
abstract: Despite the effectiveness and safety of anesthetics, some unanswered questions remain concerning their toxicity and effects on cellular redox balance. To test for possible toxic effects of balanced anesthesia maintained with the volatile anesthetic sevoflurane, we evaluated oxidative stress during and after general anesthesia in 15 adult patients without comorbidities who underwent elective minor surgical procedures. Venous blood samples were collected at baseline, before anesthesia (t0); after anesthesia induction and immediately before surgery (t1); 2h after the beginning of anesthesia (t2); and on the day following surgery (t3). Antioxidant defense was determined by fluorometry. Oxidative stress markers included oxidative DNA damage, evaluated by the alkaline comet assay, and plasma malondialdehyde (MDA), assessed by high performance liquid chromatography (HPLC). No increase in oxidized DNA damage or antioxidant defense was observed. Plasma MDA increased only at t3 compared with t2. Balanced sevoflurane-maintained anesthesia appears neither to damage DNA nor to alter redox status.
authors.affiliation: Faculdade de Medicina de Botucatu, UNESP - Univ Estadual Paulista, Departamento de Anestesiologia, Botucatu, São Paulo, Brazil.
authors.forename: José Eduardo B
authors.initials: JE
authors.lastname: Orosz
authors.affiliation: Faculdade de Medicina de Botucatu, UNESP - Univ Estadual Paulista, Departamento de Anestesiologia, Botucatu, São Paulo, Brazil.
authors.forename: Leandro G
authors.initials: LG
authors.lastname: Braz
authors.affiliation: Faculdade de Medicina de Botucatu, UNESP - Univ Estadual Paulista, Departamento de Clínica Médica, Botucatu, São Paulo, Brazil.
authors.forename: Ana Lucia A
authors.initials: AL
authors.lastname: Ferreira
authors.affiliation: Faculdade de Medicina de Botucatu, UNESP - Univ Estadual Paulista, Departamento de Anestesiologia, Botucatu, São Paulo, Brazil.
authors.forename: Rosa Beatriz
authors.initials: RB
authors.lastname: Amorim
authors.affiliation: Faculdade de Medicina de Botucatu, UNESP - Univ Estadual Paulista, Departamento de Patologia, Botucatu, São Paulo, Brazil.
authors.forename: Daisy Maria F
authors.initials: DM
authors.lastname: Salvadori
authors.affiliation: College of Biomedical and Health Sciences, Konkuk University, Chungcheongbuk-do, South Korea.
authors.forename: Kyung-Jin
authors.initials: KJ
authors.lastname: Yeum
authors.affiliation: Faculdade de Medicina de Botucatu, UNESP - Univ Estadual Paulista, Departamento de Anestesiologia, Botucatu, São Paulo, Brazil.
authors.forename: José Reinaldo C
authors.initials: JR
authors.lastname: Braz
authors.affiliation: Faculdade de Medicina de Botucatu, UNESP - Univ Estadual Paulista, Departamento de Anestesiologia, Botucatu, São Paulo, Brazil. Electronic address: mgbraz@hotmail.com.
authors.forename: Mariana G
authors.initials: MG
authors.lastname: Braz
mesh_terms: D000293:Adolescent; D000328:Adult; D000768:Anesthesia, General / Q000009:adverse effects / Q000379:methods; D018685:Anesthetics, Inhalation / Q000008:administration & dosage* / Q000009:adverse effects; D058126:Balanced Anesthesia / Q000009:adverse effects / Q000379:methods*; D004249:DNA Damage; D017558:Elective Surgical Procedures / Q000009:adverse effects / Q000379:methods; D005260:Female; D006801:Humans; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D008738:Methyl Ethers / Q000008:administration & dosage* / Q000009:adverse effects; D010084:Oxidation-Reduction / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects*; D011183:Postoperative Complications / Q000139:chemically induced / Q000453:epidemiology / Q000378:metabolism; D000077149:Sevoflurane; D055815:Young Adult
chemical_list: D018685:Anesthetics, Inhalation; D008738:Methyl Ethers; D000077149:Sevoflurane
keywords: Genotoxicity test; Inhalation anesthetics; Minor surgery; Oxidative stress