title: Comparison of culture methods for isolation of salmonella in yak fecal samples.
abstract: To compare the effectiveness of culture methods for identifying yak Salmonella, three selective enrichment broths (SC, TTB, MSRV) and three media (SS, XLD, CAS) for detecting Salmonella were evaluated in this study. The results showed that TTB broth was better than SC broths and MSRV broths, and SS medium has the highest isolation rate, significantly higher than those of CAS and XLD media (P < 0.05). It is worth noticing that there was no overlapping of the positive results given by TTB, SC and MSRV broths. In addition, all of the yak Salmonella isolates were detected positive by the five reported PCR assays, targeting the invA, srfC, invE, stn and 16S-23S rRNA genes. The combination of TTB and MSRV broths and SS and CAS media (or XLD) recommended in this study was relatively efficient in recovering Salmonella from yak feces, and the five PCR assays can be successfully used to identify yak Salmonella.
authors.affiliation: College of Life Science and Technology, Southwest University for Nationalities, No.16, South 4th Section 1st Ring Road, Chengdu, 610041 People's Republic of China.
authors.forename: Hua
authors.initials: H
authors.lastname: Yue
authors.affiliation: College of Life Science and Technology, Southwest University for Nationalities, No.16, South 4th Section 1st Ring Road, Chengdu, 610041 People's Republic of China.
authors.forename: Bin
authors.initials: B
authors.lastname: Zhang
authors.affiliation: College of Life Science and Technology, Southwest University for Nationalities, No.16, South 4th Section 1st Ring Road, Chengdu, 610041 People's Republic of China.
authors.forename: Xiaoxia
authors.initials: X
authors.lastname: Zhu
authors.affiliation: College of Life Science and Technology, Southwest University for Nationalities, No.16, South 4th Section 1st Ring Road, Chengdu, 610041 People's Republic of China.
authors.forename: Huanrong
authors.initials: H
authors.lastname: Zhang
authors.affiliation: College of Life Science and Technology, Southwest University for Nationalities, No.16, South 4th Section 1st Ring Road, Chengdu, 610041 People's Republic of China.
authors.forename: Cheng
authors.initials: C
authors.lastname: Tang
keywords: Culture methods; PCR assays; Salmonella; Yak