title: The role of interleukin 28B gene polymorphism in Turkish patients with hepatocellular carcinoma.
abstract: OBJECTIVE
  Multiple risk factors lead to hepatocellular carcinoma (HCC) including viral infections, mutation and single nucleotide polymorphisms (SNPs). Interleukin 28B (IL28B) gene rs12979860 polymorphism has been shown to be associated with HCC in the different populations, but its association with HCC has not been investigated in the Turkish population. We investigated whether the rs12979860 polymorphism of IL28B gene affects the risk of HCC.
  
  
  METHODS
  We performed genotyping analysis using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay in a hospital-based case-control study of 187 confirmed HCC patients and 208 healthy subjects (cancer and viral infection negative) in the Turkish population.
  
  
  RESULTS
  The allele and genotype analysis showed no significant differences between the risk of HCC and IL28B gene rs12979860 polymorphism (OR = 1.10; 95% 0.59-2.08 P = 0.76 for genotype). However, in the HBV-related HCC subgroup, the TT genotype increased a 1.46-fold the risk of developing HCC, but not statistically significant (OR = 1.46; 95% 0.71-2.97 P = 0.30). Furthermore, no significant differences were found between clinical findings, and sex in comparison with the IL28B genotypes in HCC group (P > 0.05).
  
  
  CONCLUSIONS
  Our results suggest, for the first time, that no significant association were found between IL28B rs12979860 genotypes with the risk of developing HCC in Turkish patients. Further independent investigations are required to clarify the possible role of IL28B gene rs12979860 polymorphism on the risk of developing HCC in a larger series and also in patients of different ethnic origins.
authors.affiliation: Çukurova University, Faculty of Medicine, Department of Gastroenterology, Adana, Turkey.
authors.forename: Hikmet
authors.initials: H
authors.lastname: Akkiz
authors.affiliation: Çukurova University, Faculty of Medicine, Department of Gastroenterology, Adana, Turkey.
authors.forename: Sedef
authors.initials: S
authors.lastname: Kuran
authors.affiliation: Çukurova University, Faculty of Medicine, Department of Gastroenterology, Adana, Turkey.
authors.forename: Ersin
authors.initials: E
authors.lastname: Akgöllü
authors.affiliation: Çukurova University, Faculty of Medicine, Department of Gastroenterology, Adana, Turkey.
authors.forename: Oguz
authors.initials: O
authors.lastname: Usküdar
authors.affiliation: Çukurova University, Faculty of Medicine, Department of Gastroenterology, Adana, Turkey.
authors.forename: Aynur
authors.initials: A
authors.lastname: Bekar
authors.affiliation: Adiyaman University, Adiyaman School of Health, Department of Nursing, Adiyaman, Turkey.
authors.forename: Süleyman
authors.initials: S
authors.lastname: Bayram
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D006528:Carcinoma, Hepatocellular / Q000209:etiology / Q000235:genetics* / Q000821:virology; D016022:Case-Control Studies; D005260:Female; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006509:Hepatitis B / Q000150:complications; D006526:Hepatitis C / Q000150:complications; D006801:Humans; D007372:Interferons; D007378:Interleukins / Q000235:genetics*; D008103:Liver Cirrhosis / Q000150:complications; D008113:Liver Neoplasms / Q000209:etiology / Q000235:genetics* / Q000821:virology; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D014421:Turkey; D055815:Young Adult; D000095022:Interferon Lambda
chemical_list: D007372:Interferons; D007378:Interleukins; C000624353:interferon-lambda, human; D000095022:Interferon Lambda