title: Purification, characterization, antioxidant activity and anti-aging of exopolysaccharides by Flammulina velutipes SF-06.
abstract: The main objective of this work was to purify the exopolysaccharides (EPS) of Flammulina velutipes SF-06 and investigate the relationship between the different purified fractions and bioactive activity. Two fractions (EPS-1 and EPS-2) were separated and purified by DEAE-52 cellulose and Sephadex G-100 cellulose column chromatography. Monosaccharides composition analysis by gas chromatography indicated that EPS, EPS-1 and EPS-2 were heteropolysaccharides in which rhamnose was a major component. Fourier transform infrared (FT-IR) analysis detected furanose-ring in EPS-1 and EPS-2. All fractions possessed considerable antioxidant activity, while EPS-2 has stronger antioxidant activity than EPS and EPS-1 in vitro. The EPS also exhibited potent anti-aging activation in mice, such as increased catalase and total antioxidant capacity, and decreasing the malondialdehyde (MDA) content. Both the antioxidant in vitro and anti-aging in vivo potentials of EPS could be further utilized in the food industry.
authors.affiliation: College of Life Science, Shandong Agricultural University, Taian, 271018, Shandong, People's Republic of China.
authors.forename: Zhao
authors.initials: Z
authors.lastname: Ma
authors.forename: Fangyuan
authors.initials: F
authors.lastname: Cui
authors.forename: Xia
authors.initials: X
authors.lastname: Gao
authors.forename: Jianjun
authors.initials: J
authors.lastname: Zhang
authors.forename: Lan
authors.initials: L
authors.lastname: Zheng
authors.forename: Le
authors.initials: L
authors.lastname: Jia
mesh_terms: D000375:Aging / Q000187:drug effects*; D000818:Animals; D000975:Antioxidants / Q000302:isolation & purification* / Q000494:pharmacology*; D002849:Chromatography, Gas; D002853:Chromatography, Liquid; D055393:Flammulina / Q000737:chemistry*; D008315:Malondialdehyde / Q000032:analysis; D051379:Mice; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D000975:Antioxidants; D008315:Malondialdehyde