title: Current status of antisense RNA-mediated gene regulation in Listeria monocytogenes.
abstract: Listeria monocytogenes is a Gram-positive human-pathogen bacterium that served as an experimental model for investigating fundamental processes of adaptive immunity and virulence. Recent novel technologies allowed the identification of several hundred non-coding RNAs (ncRNAs) in the Listeria genome and provided insight into an unexpected complex transcriptional machinery. In this review, we discuss ncRNAs that are encoded on the opposite strand of the target gene and are therefore termed antisense RNAs (asRNAs). We highlight mechanistic and functional concepts of asRNAs in L. monocytogenes and put these in context of asRNAs in other bacteria. Understanding asRNAs will further broaden our knowledge of RNA-mediated gene regulation and may provide targets for diagnostic and antimicrobial development.
authors.affiliation: Department of Medicine, Institute of Medical Microbiology, Justus-Liebig University Giessen Giessen, Germany.
authors.forename: Tilman
authors.initials: T
authors.lastname: Schultze
authors.affiliation: Department of Medicine, Massachusetts General Hospital and Harvard Medical School Boston, MA, USA.
authors.forename: Benjamin
authors.initials: B
authors.lastname: Izar
authors.affiliation: Department of Medicine, Institute of Medical Microbiology, Justus-Liebig University Giessen Giessen, Germany.
authors.forename: Xiaoxing
authors.initials: X
authors.lastname: Qing
authors.affiliation: Department of Medicine, Institute of Medical Microbiology, Justus-Liebig University Giessen Giessen, Germany.
authors.forename: Gopala K
authors.initials: GK
authors.lastname: Mannala
authors.affiliation: Department of Medicine, Institute of Medical Microbiology, Justus-Liebig University Giessen Giessen, Germany.
authors.forename: Torsten
authors.initials: T
authors.lastname: Hain
mesh_terms: D015964:Gene Expression Regulation, Bacterial; D008089:Listeria monocytogenes / Q000235:genetics* / Q000378:metabolism; D016372:RNA, Antisense / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D012329:RNA, Bacterial / Q000145:classification / Q000235:genetics* / Q000378:metabolism
chemical_list: D016372:RNA, Antisense; D012329:RNA, Bacterial
keywords: Listeria monocytogenes; antisense RNA; asRNA; bacteria; next generation sequencing; regulation