title: [Study on the effect of vibsane-type diterpenoids of Viburnum odoratissimum on human HepG2 cell growth and its underlying mechanism].
abstract: OBJECTIVE
  To study the antiproliferation effect on HepG2 cells and its underlying mechanism of the active chemical composition of the Viburnum Odoratissimum.
  
  
  METHODS
  3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) reduction assay and trypan blue dye exclusion assay were used to assess the effect of vibsane-type diterpenoids on the proliferation of various tumor cells. Alterations in cell cycle and apoptosis were determined by flowcytometry. The enzymatic activity of caspase-3/7 was measured by Apo-ONE homogeneous Caspase-3/7 Assay kit.
  
  
  RESULTS
  Compound 1 #, a vibsane-type diterpenoid, was found to significantly inhibit the growth of HepG2 cells by anticancer proliferation activity screening. It was demonstrated that the modified groups on side chain coupled to C11 site affected the cell growth-inhibition activity of compounds by structure-activity analysis. In addition, HepG2 cell line was most sensitive to compound 1 #, which induced growth arrest of HepG2 cells in a dose- and time-dependent manner. Study on the mechanisms underlying these effects indicated that compound 1 # induced significant G0/G1 phase arrest of HepG2 cells in a time- and concentration-dependent manner. Meanwhile, It was found that higher concentrations of compound (5-10 micromol/L) caused evident increase in the unmber of apoptotic cells and dose-dependent activation of caspase-3/7.
  
  
  CONCLUSIONS
  Vibsane-type diterpenoids could significantly inhibit the growth of HCC HepG2 cells. Induction of cell cycle arrest and apoptosis may play important roles in their anticancer effects.
authors.forename: Hai-Fang
authors.initials: HF
authors.lastname: Zhang
authors.forename: Lin
authors.initials: L
authors.lastname: Wang
authors.forename: Jie
authors.initials: J
authors.lastname: Liu
authors.forename: Wen-Bin
authors.initials: WB
authors.lastname: Zhou
authors.forename: Liu-Zhen
authors.initials: LZ
authors.lastname: Zhang
authors.forename: Ya-Jun
authors.initials: YJ
authors.lastname: Shan
authors.forename: Zu-Yin
authors.initials: ZY
authors.lastname: Yu
authors.forename: Ping
authors.initials: P
authors.lastname: Liu
authors.forename: Hong-Wei
authors.initials: HW
authors.lastname: Tang
authors.forename: Yu-Wen
authors.initials: YW
authors.lastname: Cong
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D059447:Cell Cycle Checkpoints / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects*; D004224:Diterpenes / Q000494:pharmacology*; D056945:Hep G2 Cells; D006801:Humans; D027925:Viburnum / Q000737:chemistry*
chemical_list: D004224:Diterpenes