title: Immobilization of chitosan film containing semaphorin 3A onto a microarc oxidized titanium implant surface via silane reaction to improve MG63 osteogenic differentiation.
abstract: Improving osseointegration of extensively used titanium (Ti) implants still remains a main theme in implantology. Recently, grafting biomolecules onto a Ti surface has attracted more attention due to their direct participation in the osseointegration process around the implant. Semaphorin 3A (Sema3A) is a new proven osteoprotection molecule and is considered to be a promising therapeutic agent in bone diseases, but how to immobilize the protein onto a Ti surface to acquire a long-term effect is poorly defined. In our study, we tried to use chitosan to wrap Sema3A (CS/Sema) and connect to the microarc oxidized Ti surface via silane glutaraldehyde coupling. The microarc oxidization could formulate porous topography on a Ti surface, and the covalently bonded coating was homogeneously covered on the ridges between the pores without significant influence on the original topography. A burst release of Sema3A was observed in the first few days in phosphate-buffered saline and could be maintained for >2 weeks. Coating in phosphate-buffered saline containing lysozyme was similar, but the release rate was much more rapid. The coating did not significantly affect cellular adhesion, viability, or cytoskeleton arrangement, but the osteogenic-related gene expression was dramatically increased and calcium deposition was also abundantly detected. In conclusion, covalent bonding of CS/Sema could strongly improve osteogenic differentiation of osteoblasts and might be applied for Ti implant surface biofunctionalization.
authors.affiliation: State Key Laboratory of Military Stomatology, Department of Implant Dentistry, School of Stomatology, Fourth Military Medical University, Xi'an, People's Republic of China.
authors.forename: Kaixiu
authors.initials: K
authors.lastname: Fang
authors.affiliation: State Key Laboratory of Military Stomatology, Department of Prosthetic Dentistry, School of Stomatology, Fourth Military Medical University, Xi'an, People's Republic of China.
authors.forename: Wen
authors.initials: W
authors.lastname: Song
authors.affiliation: State Key Laboratory of Military Stomatology, Department of Implant Dentistry, School of Stomatology, Fourth Military Medical University, Xi'an, People's Republic of China.
authors.forename: Lifeng
authors.initials: L
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Military Stomatology, Department of Oral and Maxillofacial Surgery, School of Stomatology, Fourth Military Medical University, Xi'an, People's Republic of China.
authors.forename: Sen
authors.initials: S
authors.lastname: Jia
authors.affiliation: State Key Laboratory of Military Stomatology, Department of Implant Dentistry, School of Stomatology, Fourth Military Medical University, Xi'an, People's Republic of China.
authors.forename: Hongbo
authors.initials: H
authors.lastname: Wei
authors.affiliation: State Key Laboratory of Military Stomatology, Department of Implant Dentistry, School of Stomatology, Fourth Military Medical University, Xi'an, People's Republic of China.
authors.forename: Shuai
authors.initials: S
authors.lastname: Ren
authors.affiliation: State Key Laboratory of Military Stomatology, Department of Implant Dentistry, School of Stomatology, Fourth Military Medical University, Xi'an, People's Republic of China.
authors.forename: Xiaoru
authors.initials: X
authors.lastname: Xu
authors.affiliation: State Key Laboratory of Military Stomatology, Department of Implant Dentistry, School of Stomatology, Fourth Military Medical University, Xi'an, People's Republic of China.
authors.forename: Yingliang
authors.initials: Y
authors.lastname: Song
mesh_terms: D000199:Actins / Q000378:metabolism; D002448:Cell Adhesion / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D048271:Chitosan / Q000737:chemistry*; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D005109:Extracellular Matrix / Q000378:metabolism; D006801:Humans; D010012:Osteogenesis / Q000235:genetics; D040121:Semaphorin-3A / Q000737:chemistry* / Q000493:pharmacokinetics / Q000494:pharmacology; D012821:Silanes / Q000737:chemistry; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry
chemical_list: D000199:Actins; D020099:Coated Materials, Biocompatible; D040121:Semaphorin-3A; D012821:Silanes; D048271:Chitosan; D014025:Titanium
keywords: microarc oxidation; osteogenic differentiation; semaphorin 3A; silane reaction; titanium