title: Evaluation of potential antigenotoxic, cytotoxic and proapoptotic effects of the olive oil by-product "alperujo", hydroxytyrosol, tyrosol and verbascoside.
abstract: Olive oil is an integral ingredient of the "Mediterranean diet". The olive oil industry generates large quantities of a by-product called "alperujo" (AL) during the two-phase centrifugation system developed in the early nineties. AL could be a potent exploitable source of natural phenolic antioxidants. Our results showed that AL and its distinctive phenols hydroxytyrosol, tyrosol and verbascoside were not genotoxic in the Somatic Mutation and Recombination Test (SMART) of Drosophila melanogaster and exerted antigenotoxic activity against DNA oxidative damage generated by hydrogen peroxide (H2O2). Alperujo and hydroxytyrosol also exhibited notable antiproliferative and caspase 3-dependent proapoptotic effects toward the human tumoral cell line HL60. AL can provide a cheap and efficient source of chemopreventive phenolic compounds with strong antioxidant properties, becoming a promising and potent therapeutic drug in the future.
authors.affiliation: Department of Genetics, University of Córdoba, Campus Rabanales, 14071 Córdoba, Spain.
authors.forename: Jaouad
authors.initials: J
authors.lastname: Anter
authors.affiliation: Department of Biochemistry and Molecular Biology, School of Medicine, University of Córdoba, 14071 Córdoba, Spain.
authors.forename: Inmaculada
authors.initials: I
authors.lastname: Tasset
authors.affiliation: Department of Genetics, University of Córdoba, Campus Rabanales, 14071 Córdoba, Spain.
authors.forename: Sebastián
authors.initials: S
authors.lastname: Demyda-Peyrás
authors.affiliation: Liver Unit, Reina Sofía University Hospital, Córdoba, Spain.
authors.forename: Isidora
authors.initials: I
authors.lastname: Ranchal
authors.affiliation: Department of Genetics, University of Córdoba, Campus Rabanales, 14071 Córdoba, Spain.
authors.forename: Miguel
authors.initials: M
authors.lastname: Moreno-Millán
authors.affiliation: Department of Genetics, University of Córdoba, Campus Rabanales, 14071 Córdoba, Spain.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Romero-Jimenez
authors.affiliation: Liver Unit, Reina Sofía University Hospital, Córdoba, Spain.
authors.forename: Jordi
authors.initials: J
authors.lastname: Muntané
authors.affiliation: Department of Analytical Chemistry, University of Córdoba, Campus Rabanales, 14071 Córdoba, Spain.
authors.forename: María Dolores
authors.initials: MD
authors.lastname: Luque de Castro
authors.affiliation: Department of Genetics, University of Córdoba, Campus Rabanales, 14071 Córdoba, Spain.
authors.forename: Andrés
authors.initials: A
authors.lastname: Muñoz-Serrano
authors.affiliation: Department of Genetics, University of Córdoba, Campus Rabanales, 14071 Córdoba, Spain. Electronic address: ge1almoa@uco.es.
authors.forename: Ángeles
authors.initials: Á
authors.lastname: Alonso-Moraga
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D053148:Caspase 3 / Q000378:metabolism; D049109:Cell Proliferation / Q000187:drug effects; D004249:DNA Damage; D029721:Drosophila Proteins / Q000378:metabolism; D004331:Drosophila melanogaster; D005960:Glucosides / Q000737:chemistry / Q000494:pharmacology*; D018922:HL-60 Cells; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D000069463:Olive Oil; D016877:Oxidants / Q000494:pharmacology; D010636:Phenols / Q000737:chemistry / Q000494:pharmacology*; D010626:Phenylethyl Alcohol / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology; D010938:Plant Oils / Q000737:chemistry*; D059808:Polyphenols
chemical_list: D000975:Antioxidants; D053148:Caspase 3; D029721:Drosophila Proteins; D005960:Glucosides; D006861:Hydrogen Peroxide; D000069463:Olive Oil; D016877:Oxidants; D010636:Phenols; D010626:Phenylethyl Alcohol; D010938:Plant Oils; C005975:3,4-dihydroxyphenylethanol; C011867:4-hydroxyphenylethanol; C058956:acteoside; D059808:Polyphenols; C505407:CASP3 protein, human
keywords: Alperujo; Apoptosis; Drosophila; HL60; Phenolic antioxidants