title: [Experimental study of skin wound healing with epidermal stem cells from human hypertrophic scar in nude mice].
abstract: OBJECTIVE
  To investigate the effect of epidermal stem cells from human hypertrophic scar (HS-ESCs) on the skin wound healing in nude mice.
  
  
  METHODS
  40 mice were randomly divided into two groups as experimental group (n = 20) and control group (n = 20). Wounds, 1 cm in diameters, were made on every mouse back. The wounds were treated with HS-ESCs and erythromycin ointment in experimental group, or only with erythromycin ointment in control group. The wound healing was observed during the following 14 days. The expression of collagen-I, collagen-III, epidermal growth factor (EGF), fibroblast growth factor (FGF2) , transforming growth factor (TGFbeta1, and TGFbeta2) were studied.
  
  
  RESULTS
  The wound healing time in the experimental group was (20.8 +/- 0.84) d, which was (25.6 +/- 0.89) d in the control group. HE staining revealed that the extent of vascularization in the experimental group was 11.60 +/- 0.55, while it was 8.04 +/- 0.33 in the control group. Immunochemistry analysis showed the expression of collagen-I, collagen-III, EGF, FGF2, TGFbeta1, and TGFbeta2 in the experimental group were significantly higher, compared with those in control group (P < 0.05).
  
  
  CONCLUSIONS
  HS-ESCs may promote wound healing through enhancement of the vascularization of the wound tissue and the expression of growth factors.
authors.forename: Shuping
authors.initials: S
authors.lastname: Zhou
authors.forename: Zhilong
authors.initials: Z
authors.lastname: Huang
authors.forename: Jiguang
authors.initials: J
authors.lastname: Ma
authors.forename: Jingjing
authors.initials: J
authors.lastname: Xu
authors.forename: Jinglong
authors.initials: J
authors.lastname: Cai
authors.forename: Xianlei
authors.initials: X
authors.lastname: Zong
authors.forename: Le
authors.initials: L
authors.lastname: Du
mesh_terms: D000818:Animals; D017439:Cicatrix, Hypertrophic / Q000473:pathology*; D000078404:Epidermal Cells; D005260:Female; D006801:Humans; D008297:Male; D051379:Mice; D008819:Mice, Nude; D012867:Skin / Q000293:injuries*; D033581:Stem Cell Transplantation; D013234:Stem Cells; D014945:Wound Healing