title: Expression of reactive oxygen species-related proteins according to androgen and HER-2 status in estrogen receptor-negative breast cancer.
abstract: OBJECTIVE
  The purpose of the current study is to understand the clinicopathological implications of redox proteins in association with androgen receptor (AR) and HER-2 status in estrogen receptor (ER)-negative breast cancers through evaluation of the expression patterns of redox proteins, such as catalase, thioredoxin reductase (TxNR), glutathione S-transferase π (GSTπ), thioredoxin interacting protein (TxNIP), and manganese superoxide dismutase (MnSOD).
  
  
  METHODS
  Two hundred cases of ER-negative breast cancer samples were collected as a tissue microarray. Immunohistochemical staining was done for redox-related proteins, after which the resulting data set was organized by AR and HER-2 status.
  
  
  RESULTS
  The redox proteins that had a significant association with AR and HER-2 status were tumoral catalase (p < 0.001) and stromal GSTπ (p < 0.001). Tumoral catalase was least expressed in the AR-/HER-2- group, while stromal GSTπ was least expressed in both the AR+/HER-2- and the AR-/HER-2- groups. Stromal GSTπ was highly expressed in HER-2 positive groups (p < 0.001). Stromal GSTπ negativity and tumoral MnSOD positivity were associated with a shorter disease-free survival (p = 0.041 and p = 0.007, respectively) in univariate analysis.
  
  
  CONCLUSIONS
  ER-negative breast cancers showed different expressions of redox-related proteins according to AR and HER-2 status. Catalase expression was high in AR-negative groups, while stromal GSTπ expression was high in HER-2-positive groups.
authors.affiliation: Department of Pathology, Yonsei University College of Medicine, Severance Hospital, Seoul, South Korea.
authors.forename: Ji-Ye
authors.initials: JY
authors.lastname: Kim
authors.forename: Woo Hee
authors.initials: WH
authors.lastname: Jung
authors.forename: Ja Seung
authors.initials: JS
authors.lastname: Koo
mesh_terms: D001943:Breast Neoplasms / Q000145:classification / Q000378:metabolism* / Q000473:pathology; D002374:Catalase / Q000378:metabolism; D005260:Female; D051549:Glutathione S-Transferase pi / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D010084:Oxidation-Reduction; D017382:Reactive Oxygen Species / Q000378:metabolism*; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000378:metabolism*; D011944:Receptors, Androgen / Q000378:metabolism*; D011960:Receptors, Estrogen / Q000378:metabolism; D011980:Receptors, Progesterone / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism; D013880:Thioredoxin-Disulfide Reductase / Q000378:metabolism; D000099156:Superoxide Dismutase 2
chemical_list: D002374:Catalase; D051549:Glutathione S-Transferase pi; D017382:Reactive Oxygen Species; D018719:Erb-b2 Receptor Tyrosine Kinases; D011944:Receptors, Androgen; D011960:Receptors, Estrogen; D011980:Receptors, Progesterone; D013482:Superoxide Dismutase; D013880:Thioredoxin-Disulfide Reductase; D000099156:Superoxide Dismutase 2; C508053:ERBB2 protein, human