title: The impact of HIF1α on the Per2 circadian rhythm in renal cancer cell lines.
abstract: In mammals, the circadian rhythm central generator consists of interactions among clock genes, including Per1/2/3, Cry1/2, Bmal1, and Clock. Circadian rhythm disruption may lead to increased risk of cancer in humans, and deregulation of clock genes has been implicated in many types of cancers. Among these genes, Per2 is reported to have tumor suppressor properties, but little is known about the correlation between Per2 and HIF, which is the main target of renal cell carcinoma (RCC) therapy. In this study, the rhythmic expression of the Per2 gene was not detectable in renal cancer cell lines, with the exception of Caki-2 cells. In Caki-2 cells, HIF1α increased the amplitude of Per2 oscillation by directly binding to the HIF-binding site located on the Per2 promoter. These results indicate that HIF1α may enhance the amplitude of the Per2 circadian rhythm.
authors.affiliation: Department of Uro-oncology, Saitama Medical University International Medical Center, Saitama, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Okabe
authors.affiliation: Department of Physiology, Saitama Medical University, Saitama, Japan; Molecular Clock Project, Project Research Division, Research Center for Genomic Medicine, Saitama Medical University, Saitama, Japan.
authors.forename: Megumi
authors.initials: M
authors.lastname: Kumagai
authors.affiliation: Health Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Kagawa, Japan.
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Nakajima
authors.affiliation: Department of Uro-oncology, Saitama Medical University International Medical Center, Saitama, Japan.
authors.forename: Suguru
authors.initials: S
authors.lastname: Shirotake
authors.affiliation: Department of Uro-oncology, Saitama Medical University International Medical Center, Saitama, Japan.
authors.forename: Kiichiro
authors.initials: K
authors.lastname: Kodaira
authors.affiliation: Department of Uro-oncology, Saitama Medical University International Medical Center, Saitama, Japan.
authors.forename: Masafumi
authors.initials: M
authors.lastname: Oyama
authors.affiliation: Department of Uro-oncology, Saitama Medical University International Medical Center, Saitama, Japan.
authors.forename: Munehisa
authors.initials: M
authors.lastname: Ueno
authors.affiliation: Department of Physiology, Saitama Medical University, Saitama, Japan; Molecular Clock Project, Project Research Division, Research Center for Genomic Medicine, Saitama Medical University, Saitama, Japan.
authors.forename: Masaaki
authors.initials: M
authors.lastname: Ikeda
mesh_terms: D000818:Animals; D051784:Aryl Hydrocarbon Receptor Nuclear Translocator / Q000378:metabolism; D001483:Base Sequence; D045744:Cell Line, Tumor; D002940:Circadian Rhythm / Q000235:genetics; D017930:Genes, Reporter; D005822:Genetic Vectors / Q000378:metabolism; D006801:Humans; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000378:metabolism*; D007680:Kidney Neoplasms / Q000378:metabolism / Q000473:pathology; D051379:Mice; D008810:Mice, Inbred C57BL; D041681:NIH 3T3 Cells; D056950:Period Circadian Proteins / Q000235:genetics / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D012333:RNA, Messenger / Q000378:metabolism; D014158:Transcription, Genetic
chemical_list: D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D056950:Period Circadian Proteins; D012333:RNA, Messenger; D051784:Aryl Hydrocarbon Receptor Nuclear Translocator