title: The effects of oncolytic reovirus in canine lymphoma cell lines.
abstract: Reovirus is a potent oncolytic virus in many human neoplasms that has reached phase II and III clinical trials. Our laboratory has previously reported the oncolytic effects of reovirus in canine mast cell tumour (MCT). In order to further explore the potential of reovirus in veterinary oncology, we tested the susceptibility of reovirus in 10 canine lymphoma cell lines. Reovirus-induced cell death, virus replication and infectivity were confirmed in four cell lines with variable levels of susceptibility. The level of Ras activation varied among the cell lines with no correlation with reovirus susceptibility. Reovirus-susceptible cell lines underwent apoptosis as proven by propidium iodide (PI) staining, Annexin V-FITC/PI assay, cleavage of PARP and inhibition of cell death by caspase inhibitor. A single intratumoral injection of reovirus suppressed the growth of canine lymphoma subcutaneous tumour in NOD/SCID mice. Unlike canine MCT, canine lymphoma is less susceptible to reovirus.
authors.affiliation: Laboratory of Molecular Diagnostics and Therapeutics, The United Graduate School of Veterinary Science, Yamaguchi University, Yamaguchi, Japan.
authors.forename: C C
authors.initials: CC
authors.lastname: Hwang
authors.affiliation: Laboratory of Molecular Diagnostics and Therapeutics, The United Graduate School of Veterinary Science, Yamaguchi University, Yamaguchi, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Umeki
authors.affiliation: Laboratory of Molecular Diagnostics and Therapeutics, Joint Faculty of Veterinary Medicine, Yamaguchi University, Yamaguchi, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Igase
authors.affiliation: Oncolytics Biotech Inc., Calgary, Alberta, Canada.
authors.forename: M
authors.initials: M
authors.lastname: Coffey
authors.affiliation: Laboratory of Molecular Diagnostics and Therapeutics, Joint Faculty of Veterinary Medicine, Yamaguchi University, Yamaguchi, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Noguchi
authors.affiliation: Laboratory of Veterinary Internal Medicine, Joint Faculty of Veterinary Medicine, Yamaguchi University, Yamaguchi, Japan.|Biomedical Science Center for Translational Research, The United Graduate School of Veterinary Science, Yamaguchi University, Yamaguchi, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Okuda
authors.affiliation: Laboratory of Molecular Diagnostics and Therapeutics, The United Graduate School of Veterinary Science, Yamaguchi University, Yamaguchi, Japan.|Laboratory of Molecular Diagnostics and Therapeutics, Joint Faculty of Veterinary Medicine, Yamaguchi University, Yamaguchi, Japan.|Biomedical Science Center for Translational Research, The United Graduate School of Veterinary Science, Yamaguchi University, Yamaguchi, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Mizuno
mesh_terms: D000818:Animals; D015153:Blotting, Western / Q000662:veterinary; D016923:Cell Death; D045744:Cell Line, Tumor / Q000821:virology; D004283:Dog Diseases / Q000473:pathology* / Q000821:virology*; D004285:Dogs; D008228:Lymphoma, Non-Hodgkin / Q000473:pathology / Q000662:veterinary* / Q000821:virology; D050130:Oncolytic Virotherapy / Q000379:methods / Q000662:veterinary*; D012087:Reoviridae / Q000502:physiology*
keywords: Ras; apoptosis; canine lymphoma; oncolytic virotherapy; reovirus