title: Identification of receptor protein tyrosine phosphatases (RPTPs) as regulators of receptor tyrosine kinases (RTKs) using an RPTP siRNA-RTK substrate screen.
abstract: Receptor tyrosine kinase (RTK) signaling exists in equilibrium between RTK tyrosyl phosphorylation and RTK tyrosyl dephosphorylation. Despite a detailed understanding of RTK tyrosyl phosphorylation, much less is known about RTK tyrosyl dephosphorylation. The receptor PTPs (RPTPs) are outstanding targets for the dephosphorylation of RTKs because of their mutual membrane proximity. In this chapter, we describe how to identify RPTPs that modulate the activity of RTKs using a siRNA screen and commercially available proteomic applications. The validation of putative RTKs as RPTP substrates using substrate-trapping approaches is detailed.
authors.affiliation: Department of Pharmacology, Yale University School of Medicine, SHM B226D, 333 Cedar Street, New Haven, CT, 06520-8066, USA.
authors.forename: Hojin
authors.initials: H
authors.lastname: Lee
authors.forename: Anton M
authors.initials: AM
authors.lastname: Bennett
mesh_terms: D045163:Antibodies, Phospho-Specific / Q000737:chemistry; D002460:Cell Line; D005786:Gene Expression Regulation; D057166:High-Throughput Screening Assays; D006801:Humans; D010766:Phosphorylation; D040081:Protein Array Analysis / Q000295:instrumentation; D017027:Protein Tyrosine Phosphatases / Q000235:genetics / Q000378:metabolism*; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism*; D020794:Receptor Protein-Tyrosine Kinases / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction
chemical_list: D045163:Antibodies, Phospho-Specific; D034741:RNA, Small Interfering; D011993:Recombinant Fusion Proteins; D020794:Receptor Protein-Tyrosine Kinases; D017027:Protein Tyrosine Phosphatases