title: Differential sensing of MAP kinases using SOX-peptides.
abstract: Five SOX peptides are used to classify the MAPK groups and isoforms thereof using chemometrics. The score plots show excellent classification and accuracy, while support vector machine analysis leads to the quantification of ERK and an ERK inhibitor concentration in kinase mixtures. Examination of the loading plots reveals cross-reactivity among the peptides, and some unexpected surprises.
authors.affiliation: Department of Chemistry and Biochemistry, The University of Texas at Austin, 1 University Station A1590, Austin, Texas 78712 (USA).
authors.forename: Diana
authors.initials: D
authors.lastname: Zamora-Olivares
authors.forename: Tamer S
authors.initials: TS
authors.lastname: Kaoud
authors.forename: Jiney
authors.initials: J
authors.lastname: Jose
authors.forename: Andrew
authors.initials: A
authors.lastname: Ellington
authors.forename: Kevin N
authors.initials: KN
authors.lastname: Dalby
authors.forename: Eric V
authors.initials: EV
authors.lastname: Anslyn
mesh_terms: D020928:Mitogen-Activated Protein Kinases / Q000037:antagonists & inhibitors / Q000737:chemistry* / Q000378:metabolism; D015394:Molecular Structure; D010455:Peptides / Q000737:chemistry / Q000494:pharmacology*; D013449:Sulfonamides / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D010455:Peptides; D013449:Sulfonamides; D020928:Mitogen-Activated Protein Kinases
keywords: biosensors; chemometrics; differential sensing; fluorescent probes; mitogen-activated protein kinases