title: Overexpression of a miR393-resistant form of transport inhibitor response protein 1 (mTIR1) enhances salt tolerance by increased osmoregulation and Na+ exclusion in Arabidopsis thaliana.
abstract: Soil salinity is a common environmental stress factor that limits agricultural production worldwide. Plants have evolved different strategies to achieve salt tolerance. miR393 has been identified as closely related to biotic and abiotic stresses, and targets F-box genes that encode auxin receptors. The miR393-TIR1/AFB2/AFB3 regulatory module was discovered to have multiple functions that manipulate the auxin response. This study focused on miR393 and one of its targets, TIR1, and found that they played potential roles in response to salt stress. Our results showed that overexpression of a miR393-resistant TIR1 gene (mTIR1) in Arabidopsis clearly enhanced salt stress tolerance, which led to a higher germination rate, less water loss, reduced inhibition of root elongation, delayed senescence, decreased death rate and stabilized Chl content. These plants accumulated more proline and anthocyanin, and displayed enhanced osmotic stress tolerance. The expression of some salt stress-related genes was altered, and sodium content can be reduced in these plants under salt stress. We proposed that highly increased auxin signaling by overexpression of mTIR1 may trigger auxin-mediated downstream pathways to enhance plant salt stress resistance by osmoregulation and increased Na(+) exclusion.
authors.affiliation: College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, 310036, Zhejiang, China These authors contributed equally to this work.
authors.forename: Zhehao
authors.initials: Z
authors.lastname: Chen
authors.affiliation: College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, 310036, Zhejiang, China These authors contributed equally to this work.
authors.forename: Lingzhi
authors.initials: L
authors.lastname: Hu
authors.affiliation: State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou, 310058, Zhejiang, China.
authors.forename: Ning
authors.initials: N
authors.lastname: Han
authors.affiliation: College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, 310036, Zhejiang, China.
authors.forename: Jiangqin
authors.initials: J
authors.lastname: Hu
authors.affiliation: College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, 310036, Zhejiang, China.
authors.forename: Yanjun
authors.initials: Y
authors.lastname: Yang
authors.affiliation: College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, 310036, Zhejiang, China.
authors.forename: Taihe
authors.initials: T
authors.lastname: Xiang
authors.affiliation: College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, 310036, Zhejiang, China.
authors.forename: Xujia
authors.initials: X
authors.lastname: Zhang
authors.affiliation: College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, 310036, Zhejiang, China wang_208@163.com.
authors.forename: Lilin
authors.initials: L
authors.lastname: Wang
mesh_terms: D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics* / Q000502:physiology; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism; D044783:F-Box Proteins / Q000235:genetics* / Q000378:metabolism; D015870:Gene Expression; D018506:Gene Expression Regulation, Plant; D017930:Genes, Reporter; D018525:Germination; D007210:Indoleacetic Acids / Q000378:metabolism; D035683:MicroRNAs / Q000235:genetics*; D064587:Osmoregulation; D018517:Plant Roots / Q000187:drug effects / Q000235:genetics / Q000502:physiology; D030821:Plants, Genetically Modified; D011956:Receptors, Cell Surface / Q000235:genetics* / Q000378:metabolism; D055049:Salt Tolerance; D036226:Seedlings / Q000187:drug effects / Q000235:genetics / Q000502:physiology; D012964:Sodium / Q000378:metabolism*; D012965:Sodium Chloride / Q000494:pharmacology; D013312:Stress, Physiological
chemical_list: C000603490:AFB2 protein, Arabidopsis; C000596911:AFB3 protein, Arabidopsis; D029681:Arabidopsis Proteins; D044783:F-Box Proteins; D007210:Indoleacetic Acids; C559857:MIRN393 microRNA, Arabidopsis; D035683:MicroRNAs; D011956:Receptors, Cell Surface; C501405:TIR1 protein, Arabidopsis; D012965:Sodium Chloride; D012964:Sodium
keywords: Arabidopsis thaliana; MiR393–TIR1; Osmoregulation; Salinity; Tolerance