title: (1)H, (13)C, and (15)N resonance assignments for the tandem PHD finger motifs of human CHD4.
abstract: The plant homeodomain (PHD) zinc finger is a structural motif of about 40-60 amino acid residues found in many eukaryotic proteins that are involved in chromatin-mediated gene regulation. The human chromodomain helicase DNA binding protein 4 (CHD4) is a multi-domain protein that harbours, at its N-terminal end, a pair of PHD finger motifs (dPHD) connected by a ~30 amino acid linker. This tandem PHD motif is thought to be involved in targeting CHD4 to chromatin via its interaction with histone tails. Here we report the (1)H, (13)C and (15)N backbone and side-chain resonance assignment of the entire dPHD by heteronuclear multidimensional NMR spectroscopy. These assignments provide the starting point for the determination of the structure, dynamics and histone-binding properties of this tandem domain pair.
authors.affiliation: Department of Biochemistry, University of Oxford, South Parks Road, Oxford, OX1 3QU, UK.
authors.forename: Louise J
authors.initials: LJ
authors.lastname: Walport
authors.affiliation: Division of Structural Biology, Henry Wellcome Building for Genomic Medicine, University of Oxford, Roosevelt Drive, Oxford, OX3 7BN, UK.
authors.forename: Rosa
authors.initials: R
authors.lastname: Morra
authors.affiliation: Division of Structural Biology, Henry Wellcome Building for Genomic Medicine, University of Oxford, Roosevelt Drive, Oxford, OX3 7BN, UK. erika@strubi.ox.ac.uk.
authors.forename: Erika J
authors.initials: EJ
authors.lastname: Mancini
authors.affiliation: Department of Biochemistry, University of Oxford, South Parks Road, Oxford, OX1 3QU, UK. christina.redfield@bioch.ox.ac.uk.
authors.forename: Christina
authors.initials: C
authors.lastname: Redfield
mesh_terms: D020816:Amino Acid Motifs; D001324:Autoantigens / Q000737:chemistry*; D066241:Carbon-13 Magnetic Resonance Spectroscopy; D006801:Humans; D056245:Mi-2 Nucleosome Remodeling and Deacetylase Complex / Q000737:chemistry*; D009587:Nitrogen Isotopes; D011392:Proline / Q000737:chemistry; D017434:Protein Structure, Tertiary; D066244:Proton Magnetic Resonance Spectroscopy
chemical_list: D001324:Autoantigens; C115323:CHD4 protein, human; D009587:Nitrogen Isotopes; D011392:Proline; D056245:Mi-2 Nucleosome Remodeling and Deacetylase Complex
keywords: Backbone resonance assignments; Heteronuclear NMR; Histones; Human chromodomain helicase DNA binding protein 4; Plant homeodomain zinc finger