title: Second generation genetic linkage map for the gilthead sea bream Sparus aurata L.
abstract: An updated second linkage map was constructed for the gilthead sea bream, Sparus aurata L., a fish species of great economic importance for the Mediterranean aquaculture industry. In contrast to the first linkage map which mainly consisted of genomic microsatellites (SSRs), the new linkage map is highly enriched with SSRs found in Expressed Sequence Tags (EST-SSRs), which greatly facilitates comparative mapping with other teleosts. The new map consists of 321 genetic markers in 27 linkage groups (LGs): 232 genomic microsatellites, 85 EST-SSRs and 4 SNPs; of those, 13 markers were linked to LGs but were not ordered. Eleven markers (5 SSRs, 5 EST-SSRs and 1 SNP) are not assigned to any LG. The total length of the sex-averaged map is 1769.7cM, 42% longer than the previously published one, and the number of markers in each LG ranges from 2 to 30. The inter-marker distance varies from 0 to 75.6cM, with an average of 5.75cM. The male and female maps have a length of 1349.2 and 2172.1cM, respectively, and the average distance between markers is 4.38 and 7.05cM, respectively. Comparative mapping with the three-spined stickleback (Gasterosteus acuulatus) chromosomes and scaffolds showed conserved synteny with 132 S. aurata markers (42.9% of those mapped) having a hit on the stickleback genome.
authors.affiliation: Institute of Marine Biology, Biotechnology and Aquaculture (IMBBC), Hellenic Centre for Marine Research (HCMR), Thalassocosmos, Ex-US base at Gournes Pediados, 715 00 Heraklion, Crete, Greece. Electronic address: tsigeno@hcmr.gr.
authors.forename: Costas S
authors.initials: CS
authors.lastname: Tsigenopoulos
authors.affiliation: Centro de Ciências do Mar do Algarve, Universidade do Algarve, Campus de Gambelas, 8005-139 Faro, Portugal.
authors.forename: Bruno
authors.initials: B
authors.lastname: Louro
authors.affiliation: Department of Agricultural Technology, School of Agricultural Technology Food Technology and Nutrition, Alexander Technological Educational Institute of Thessaloniki, 57 400 Sindos, Greece.
authors.forename: Dimitrios
authors.initials: D
authors.lastname: Chatziplis
authors.affiliation: Institute of Marine Biology, Biotechnology and Aquaculture (IMBBC), Hellenic Centre for Marine Research (HCMR), Thalassocosmos, Ex-US base at Gournes Pediados, 715 00 Heraklion, Crete, Greece.
authors.forename: Jacques
authors.initials: J
authors.lastname: Lagnel
authors.affiliation: Institute of Marine Biology, Biotechnology and Aquaculture (IMBBC), Hellenic Centre for Marine Research (HCMR), Thalassocosmos, Ex-US base at Gournes Pediados, 715 00 Heraklion, Crete, Greece.
authors.forename: Emmanouella
authors.initials: E
authors.lastname: Vogiatzi
authors.affiliation: Department of Agricultural Technology, School of Agricultural Technology Food Technology and Nutrition, Alexander Technological Educational Institute of Thessaloniki, 57 400 Sindos, Greece.
authors.forename: Dimitrios
authors.initials: D
authors.lastname: Loukovitis
authors.affiliation: Department of Public Health, Comparative Pathology and Veterinary Hygiene, University of Padova, 35020 Legnaro, Italy.
authors.forename: Rafaella
authors.initials: R
authors.lastname: Franch
authors.affiliation: Institute of Marine Biology, Biotechnology and Aquaculture (IMBBC), Hellenic Centre for Marine Research (HCMR), Thalassocosmos, Ex-US base at Gournes Pediados, 715 00 Heraklion, Crete, Greece.
authors.forename: Elena
authors.initials: E
authors.lastname: Sarropoulou
authors.affiliation: Centro de Ciências do Mar do Algarve, Universidade do Algarve, Campus de Gambelas, 8005-139 Faro, Portugal.
authors.forename: Deborah M
authors.initials: DM
authors.lastname: Power
authors.affiliation: Department of Public Health, Comparative Pathology and Veterinary Hygiene, University of Padova, 35020 Legnaro, Italy.
authors.forename: Tomaso
authors.initials: T
authors.lastname: Patarnello
authors.affiliation: Institute of Marine Biology, Biotechnology and Aquaculture (IMBBC), Hellenic Centre for Marine Research (HCMR), Thalassocosmos, Ex-US base at Gournes Pediados, 715 00 Heraklion, Crete, Greece.
authors.forename: Constantinos C
authors.initials: CC
authors.lastname: Mylonas
authors.affiliation: Institute of Marine Biology, Biotechnology and Aquaculture (IMBBC), Hellenic Centre for Marine Research (HCMR), Thalassocosmos, Ex-US base at Gournes Pediados, 715 00 Heraklion, Crete, Greece.
authors.forename: Antonios
authors.initials: A
authors.lastname: Magoulas
authors.affiliation: Department of Public Health, Comparative Pathology and Veterinary Hygiene, University of Padova, 35020 Legnaro, Italy.
authors.forename: Luca
authors.initials: L
authors.lastname: Bargelloni
authors.affiliation: Centro de Ciências do Mar do Algarve, Universidade do Algarve, Campus de Gambelas, 8005-139 Faro, Portugal.
authors.forename: Adelino
authors.initials: A
authors.lastname: Canario
authors.affiliation: Institute of Marine Biology, Biotechnology and Aquaculture (IMBBC), Hellenic Centre for Marine Research (HCMR), Thalassocosmos, Ex-US base at Gournes Pediados, 715 00 Heraklion, Crete, Greece.
authors.forename: Georgios
authors.initials: G
authors.lastname: Kotoulas
mesh_terms: D000818:Animals; D002874:Chromosome Mapping; D005260:Female; D008040:Genetic Linkage; D005819:Genetic Markers / Q000235:genetics; D008297:Male; D021541:Sea Bream / Q000235:genetics*; D023701:Smegmamorpha / Q000235:genetics
chemical_list: D005819:Genetic Markers
keywords: Comparative map; EST-SSR; Microsatellites; SNP; Sparus aurata; Synteny