title: Guidance of mesenchymal stem cells on fibronectin structured hydrogel films.
abstract: Designing of implant surfaces using a suitable ligand for cell adhesion to stimulate specific biological responses of stem cells will boost the application of regenerative implants. For example, materials that facilitate rapid and guided migration of stem cells would promote tissue regeneration. When seeded on fibronectin (FN) that was homogeneously immmobilized to NCO-sP(EO-stat-PO), which otherwise prevents protein binding and cell adhesion, human mesenchymal stem cells (MSC) revealed a faster migration, increased spreading and a more rapid organization of different cellular components for cell adhesion on fibronectin than on a glass surface. To further explore, how a structural organization of FN controls the behavior of MSC, adhesive lines of FN with varying width between 10 µm and 80 µm and spacings between 5 µm and 20 µm that did not allow cell adhesion were generated. In dependance on both line width and gaps, cells formed adjacent cell contacts, were individually organized in lines, or bridged the lines. With decreasing sizes of FN lines, speed and directionality of cell migration increased, which correlated with organization of the actin cytoskeleton, size and shape of the nuclei as well as of focal adhesions. Together, defined FN lines and gaps enabled a fine tuning of the structural organization of cellular components and migration. Microstructured adhesive substrates can mimic the extracellular matrix in vivo and stimulate cellular mechanisms which play a role in tissue regeneration.
authors.affiliation: Laboratory of Cell Biology, Rostock University Medical Center, Rostock, Germany.
authors.forename: Annika
authors.initials: A
authors.lastname: Kasten
authors.affiliation: Division for Biochemistry of Joint and Connective Tissue Diseases of the Orthopedic Department, University of Ulm, Ulm, Germany.
authors.forename: Tamara
authors.initials: T
authors.lastname: Naser
authors.affiliation: DWI Leibniz Institute for Interactive Materials and Institute of Technical and Macromolecular Chemistry, RWTH Aachen University, Aachen, Germany.
authors.forename: Kristina
authors.initials: K
authors.lastname: Brüllhoff
authors.affiliation: Division for Biochemistry of Joint and Connective Tissue Diseases of the Orthopedic Department, University of Ulm, Ulm, Germany.
authors.forename: Jörg
authors.initials: J
authors.lastname: Fiedler
authors.affiliation: Laboratory of Cell Biology, Rostock University Medical Center, Rostock, Germany.
authors.forename: Petra
authors.initials: P
authors.lastname: Müller
authors.affiliation: DWI Leibniz Institute for Interactive Materials and Institute of Technical and Macromolecular Chemistry, RWTH Aachen University, Aachen, Germany.
authors.forename: Martin
authors.initials: M
authors.lastname: Möller
authors.affiliation: Laboratory of Cell Biology, Rostock University Medical Center, Rostock, Germany.
authors.forename: Joachim
authors.initials: J
authors.lastname: Rychly
authors.affiliation: DWI Leibniz Institute for Interactive Materials and Institute of Technical and Macromolecular Chemistry, RWTH Aachen University, Aachen, Germany; Department and Chair of Functional Materials in Medicine and Dentistry, University of Würzburg, Würzburg, Germany.
authors.forename: Jürgen
authors.initials: J
authors.lastname: Groll
authors.affiliation: Division for Biochemistry of Joint and Connective Tissue Diseases of the Orthopedic Department, University of Ulm, Ulm, Germany.
authors.forename: Rolf E
authors.initials: RE
authors.lastname: Brenner
mesh_terms: D000199:Actins / Q000378:metabolism; D002448:Cell Adhesion / Q000187:drug effects*; D002460:Cell Line; D002465:Cell Movement / Q000187:drug effects; D003599:Cytoskeleton / Q000187:drug effects; D005109:Extracellular Matrix / Q000187:drug effects / Q000502:physiology; D005353:Fibronectins / Q000494:pharmacology*; D006801:Humans; D008024:Ligands; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects; D012038:Regeneration
chemical_list: D000199:Actins; D005353:Fibronectins; D008024:Ligands