title: Phylogeography of Tetrancistrum nebulosi (Monogenea, Dactylogyridae) on the host of mottled spinefoot (Siganus fuscescens) in the South China Sea, inferred from mitochondrial COI and ND2 genes.
abstract: To examine the phylogeographical pattern of Tetrancistrum nebulosi (Monogenea, Dactylogyridae) in the South China Sea, fragments of mitochondrial cytochrome c oxidase subunit I and NADH dehydrogenase subunit 2 genes were obtained for 220 individuals collected from 8 localities along the southeast coast of China and 1 locality in Terengganu, Malaysia. Based on these two genes, two and three distinct clades with geographic signals were revealed on the phylogenetic trees respectively. The divergence between these clades was estimated to occur in the late Pleistocene. Analysis of molecular variance and pairwise FSTsuggested a high rate of gene flow among individuals sampled from the Chinese coast, but with obvious genetic differentiation from the Malaysian population. Mismatch distribution and neutrality tests indicated that the T. nebulosi population experienced expansion in Pleistocene low sea level periods. Vicariance was considered to account for the genetic divergence between Chinese and Malaysian populations, while sea level fluctuations and mainland-island connections during glacial cycles were associated with the slight genetic divergence between the populations along the mainland coast of China and those off Sanya. On the contrary, oceanographic circulations and host migration could lead to genetic homogeneity of populations distributed along the mainland coast of China.
authors.affiliation: a State Key Laboratory of Biocontrol and Center for Parasitic Organisms , School of Life Sciences, Sun Yat-sen University , Guangzhou , P.R. China.
authors.forename: Ming
authors.initials: M
authors.lastname: Wang
authors.affiliation: a State Key Laboratory of Biocontrol and Center for Parasitic Organisms , School of Life Sciences, Sun Yat-sen University , Guangzhou , P.R. China.
authors.forename: Shuai
authors.initials: S
authors.lastname: Yan
authors.affiliation: b The Milford Laboratory, Aquaculture and Enhancement Division , National Oceanic and Atmospheric Administration , Milford , USA , and.
authors.forename: Christopher L
authors.initials: CL
authors.lastname: Brown
authors.affiliation: c Institute of Tropical Aquaculture, Universiti Malaysia Terengganu , Kuala Terengganu , Malaysia.
authors.forename: Faizah
authors.initials: F
authors.lastname: Shaharom-Harrison
authors.affiliation: a State Key Laboratory of Biocontrol and Center for Parasitic Organisms , School of Life Sciences, Sun Yat-sen University , Guangzhou , P.R. China.
authors.forename: Su-Fen
authors.initials: SF
authors.lastname: Shi
authors.affiliation: a State Key Laboratory of Biocontrol and Center for Parasitic Organisms , School of Life Sciences, Sun Yat-sen University , Guangzhou , P.R. China.
authors.forename: Ting-Bao
authors.initials: TB
authors.lastname: Yang
mesh_terms: D000818:Animals; D002681:China; D004272:DNA, Mitochondrial / Q000737:chemistry / Q000235:genetics; D003576:Electron Transport Complex IV / Q000235:genetics; D005393:Fish Diseases / Q000469:parasitology*; D051456:Gene Flow; D014644:Genetic Variation; D054629:Genome, Mitochondrial / Q000235:genetics*; D005880:Gills / Q000469:parasitology; D008296:Malaysia; D008928:Mitochondria / Q000235:genetics; D024101:Mitochondrial Proteins / Q000235:genetics; D009245:NADH Dehydrogenase / Q000235:genetics; D010473:Perciformes / Q000469:parasitology*; D058974:Phylogeography; D010986:Platyhelminths / Q000235:genetics*; D017422:Sequence Analysis, DNA / Q000662:veterinary
chemical_list: D004272:DNA, Mitochondrial; D024101:Mitochondrial Proteins; D009245:NADH Dehydrogenase; D003576:Electron Transport Complex IV
keywords: COI; Monogenea; ND2; Tetrancistrum nebulosi; pleistocene isolation