title: microRNA133a targets Foxl2 and promotes differentiation of C2C12 into myogenic progenitor cells.
abstract: microRNAs are endogenous noncoding RNA molecules of ∼22 nucleotides that regulate gene function by modification of target mRNAs. Due to tissue specific of miR-133a and miR-1/206 for skeletal muscles, we investigated the role of miR-133a and miR-1/206 in promoting the differentiation of the C2C12 cells. The results show that directly transfecting mature miR-133a, miR-1/206, or combinations (miR-1 and miR-206, miR-1 and miR-133a, and miR-133a and miR-206) into C2C12 cells, respectively, for 5 days induces formation of myogenic progenitor cells. Overexpression of miR-133a and miR-206 in C2C12 cells greatly improved multinucleated myotube formation. microRNA-133a (miR-133a) is highly expressed during human muscle development. Using bioinformatics, we identified one putative miR-133a binding site within the 3'-untranslated region of the mouse Foxl2 mRNA. The expression of Foxl2 was shown to be downregulated by subsequent western blot analysis.
authors.affiliation: 1 State Key Laboratory for Diagnosis and Treatment of Infectious Disease, The First Affiliated Hospital, School of Medicine, Zhejiang University , Hangzhou, People's Republic of China .
authors.forename: Yueqiu
authors.initials: Y
authors.lastname: Luo
authors.forename: Xiaoxing
authors.initials: X
authors.lastname: Wu
authors.forename: Zongxin
authors.initials: Z
authors.lastname: Ling
authors.forename: Li
authors.initials: L
authors.lastname: Yuan
authors.forename: Yiwen
authors.initials: Y
authors.lastname: Cheng
authors.forename: Jingyang
authors.initials: J
authors.lastname: Chen
authors.forename: Charlie
authors.initials: C
authors.lastname: Xiang
mesh_terms: D020413:3' Untranslated Regions; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D002454:Cell Differentiation; D002460:Cell Line; D000074842:Forkhead Box Protein L2; D051858:Forkhead Transcription Factors / Q000235:genetics* / Q000378:metabolism; D015870:Gene Expression; D057809:HEK293 Cells; D006801:Humans; D051379:Mice; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D032448:Myoblasts, Skeletal / Q000378:metabolism*; D034622:RNA Interference
chemical_list: D020413:3' Untranslated Regions; D000074842:Forkhead Box Protein L2; D051858:Forkhead Transcription Factors; C498893:Foxl2 protein, mouse; D035683:MicroRNAs; C517732:Mirn133 microRNA, mouse; C540669:Mirn206 microRNA, mouse