title: 3D super-resolution imaging by localization microscopy.
abstract: Fluorescence microscopy is an important tool in all fields of biology to visualize structures and monitor dynamic processes and distributions. Contrary to conventional microscopy techniques such as confocal microscopy, which are limited by their spatial resolution, super-resolution techniques such as photoactivated localization microscopy (PALM) and stochastic optical reconstruction microscopy (STORM) have made it possible to observe and quantify structure and processes on the single molecule level. Here, we describe a method to image and quantify the molecular distribution of membrane-associated proteins in two and three dimensions with nanometer resolution.
authors.affiliation: Centre for Vascular Research, University of New South Wales, High Street, Kensington, Sydney, NSW, 2052, Australia, a.magenau@garvan.org.au.
authors.forename: Astrid
authors.initials: A
authors.lastname: Magenau
authors.forename: Katharina
authors.initials: K
authors.lastname: Gaus
mesh_terms: D018274:Electroporation / Q000295:instrumentation / Q000379:methods; D006801:Humans; D021621:Imaging, Three-Dimensional / Q000379:methods*; D019169:Jurkat Cells; D008853:Microscopy / Q000379:methods; D008856:Microscopy, Fluorescence / Q000295:instrumentation / Q000379:methods*; D011506:Proteins / Q000032:analysis; D014162:Transfection / Q000295:instrumentation / Q000379:methods
chemical_list: D011506:Proteins