title: Heparan sulfate modulates Slit3-induced endothelial cell migration.
abstract: Heparan sulfate is a long, linear polysaccharide with sulfation modifications and belongs to the glycosaminoglycan family. Our recent studies elucidated that the axon guidance molecule Slit3 is a new heparan sulfate-binding protein and a novel angiogenic factor by interacting with its cognate receptor Robo4, which is specifically expressed in endothelial cells. Here we describe using heparan sulfate-deficient mouse endothelial cells to determine the co-reception function of heparan sulfate in Slit3-induced endothelial cell migration in a Boyden chamber trans-well migration assay.
authors.affiliation: Department of Biochemistry and Molecular Biology, Complex Carbohydrate Research Center, University of Georgia, 315 Riverbend Road, Athens, GA, 30602-4712, USA.
authors.forename: Hong
authors.initials: H
authors.lastname: Qiu
authors.forename: Wenyuan
authors.initials: W
authors.lastname: Xiao
authors.forename: Jingwen
authors.initials: J
authors.lastname: Yue
authors.forename: Lianchun
authors.initials: L
authors.lastname: Wang
mesh_terms: D000818:Animals; D002465:Cell Movement; D042783:Endothelial Cells / Q000166:cytology* / Q000378:metabolism*; D006497:Heparan Sulfate / Q000378:metabolism*; D008565:Membrane Proteins / Q000378:metabolism*; D051379:Mice; D013223:Statistics as Topic; D034421:Sus scrofa
chemical_list: D006497:Heparan Sulfate; D008565:Membrane Proteins; C432251:Slit3 protein, mouse