title: Directing the fate of human and mouse mesenchymal stem cells by hydroxyl-methyl mixed self-assembled monolayers with varying wettability.
abstract: Self-assembled monolayers (SAMs) of alkanethiols on gold have been employed as model substrates to investigate the effects of surface chemistry on cell behavior. However, few studies were dedicated to the substrates with a controlled wettability in studying stem cell fate. Here, mixed hydroxyl (-OH) and methyl (-CH3) terminated SAMs were prepared to form substrates with varying wettability, which were used to study the effects of wettability on the adhesion, spreading, proliferation and osteogenic differentiation of mesenchymal stem cells (MSCs) from human and mouse origins. The numbers of adhered human fetal MSCs (hMSCs) and mouse bone marrow MSCs (mMSCs) were maximized on -OH/-CH3mixed SAMs with a water contact angle of 40~70° and 70~90°, respectively. Hydrophilic mixed SAMs with a water contact angle of 20~70° also promoted the spreading of both hMSCs and mMSCs. Both hMSCs and mMSCs proliferation was most favored on hydrophilic SAMs with a water contact angle around 70°. In addition, a moderate hydrophilic surface (with a contact angle of 40~90° for hMSCs and 70° for mMSCs) promoted osteogenic differentiation in the presence of biological stimuli. Hydrophilic mixed SAMs with a moderate wettability tended to promote the expression of αvβ1integrin of MSCs, indicating that the tunable wettability of the mixed SAMs may guide osteogenesis through mediating the αvβ1integrin signaling pathway. Our work can direct the design of biomaterials with controllable wettability to promote the adhesion, proliferation and differentiation of MSCs from different sources.
authors.affiliation: School of Materials Science and Engineering, South China University of Technology, Guangzhou 510641, China ; National Engineering Research Center for Tissue Restoration and Reconstruction, Guangzhou 510006, China.
authors.forename: Lijing
authors.initials: L
authors.lastname: Hao
authors.affiliation: School of Materials Science and Engineering, South China University of Technology, Guangzhou 510641, China ; National Engineering Research Center for Tissue Restoration and Reconstruction, Guangzhou 510006, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Yang
authors.affiliation: School of Materials Science and Engineering, South China University of Technology, Guangzhou 510641, China ; National Engineering Research Center for Tissue Restoration and Reconstruction, Guangzhou 510006, China.
authors.forename: Chang
authors.initials: C
authors.lastname: Du
authors.affiliation: School of Materials Science and Engineering, South China University of Technology, Guangzhou 510641, China ; National Engineering Research Center for Tissue Restoration and Reconstruction, Guangzhou 510006, China.
authors.forename: Xiaoling
authors.initials: X
authors.lastname: Fu
authors.affiliation: School of Materials Science and Engineering, South China University of Technology, Guangzhou 510641, China ; National Engineering Research Center for Tissue Restoration and Reconstruction, Guangzhou 510006, China.
authors.forename: Naru
authors.initials: N
authors.lastname: Zhao
authors.affiliation: Department of Materials Science and Engineering, Tsinghua University, Beijing 100084, PR China.
authors.forename: Suju
authors.initials: S
authors.lastname: Xu
authors.affiliation: Department of Materials Science and Engineering, Tsinghua University, Beijing 100084, PR China.
authors.forename: Fuzhai
authors.initials: F
authors.lastname: Cui
authors.affiliation: Department of Chemistry and Biochemistry, Stephenson Life Science Research Center, University of Oklahoma, Norman, OK 73019, USA.
authors.forename: Chuanbin
authors.initials: C
authors.lastname: Mao
authors.affiliation: School of Materials Science and Engineering, South China University of Technology, Guangzhou 510641, China ; National Engineering Research Center for Tissue Restoration and Reconstruction, Guangzhou 510006, China.
authors.forename: Yingjun
authors.initials: Y
authors.lastname: Wang