title: Microbial ecology in anaerobic digestion at agitated and non-agitated conditions.
abstract: To investigate the distribution and dynamics of microbial community in anaerobic digestion at agitated and non-agitated condition, 454 pyrosequencing of 16s rRNA was conducted. It revealed the distinct community compositions between the two digesters and their progressive shifting over time. Methanogens and syntrophic bacteria were found much less abundant in the agitated digester, which was mainly attributed to the presence of bacterial genera Acetanaerobacterium and Ruminococcus with relatively high abundance. The characterization of the microbial community corroborated the digestion performance affected at the agitated condition, where lower methane yield and delayed methane production rate were observed. This was further verified by the accumulation of propionic acid in the agitated digester.
authors.affiliation: Department of Agricultural and Biological Engineering, University of Florida, Gainesville, Florida, United States of America.
authors.forename: Zhuoli
authors.initials: Z
authors.lastname: Tian
authors.affiliation: Escuela de Ingeniería Bioquímica, Pontificia Universidad Catolica de Valparíaso, General Cruz 34, Valparaíso, Chile.
authors.forename: Léa
authors.initials: L
authors.lastname: Cabrol
authors.affiliation: Escuela de Ingeniería Bioquímica, Pontificia Universidad Catolica de Valparíaso, General Cruz 34, Valparaíso, Chile.
authors.forename: Gonzalo
authors.initials: G
authors.lastname: Ruiz-Filippi
authors.affiliation: Department of Agricultural and Biological Engineering, University of Florida, Gainesville, Florida, United States of America.
authors.forename: Pratap
authors.initials: P
authors.lastname: Pullammanappallil
mesh_terms: D000693:Anaerobiosis; D001105:Archaea / Q000235:genetics / Q000378:metabolism; D057919:Biological Oxygen Demand Analysis; D008697:Methane / Q000096:biosynthesis; D064307:Microbiota; D058889:Molecular Typing; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D045857:Ruminococcus / Q000235:genetics / Q000378:metabolism*; D055549:Volatile Organic Compounds / Q000378:metabolism
chemical_list: D012336:RNA, Ribosomal, 16S; D055549:Volatile Organic Compounds; D008697:Methane