title: The proliferation study of hips cell-derived neuronal progenitors on poly-caprolactone scaffold.
abstract: BACKGROUND
  The native inability of nervous system to regenerate, encourage researchers to consider neural tissue engineering as a potential treatment for spinal cord injuries. Considering the suitable characteristics of induced pluripotent stem cells (iPSCs) for tissue regeneration applications, in this study we investigated the adhesion, viability and proliferation of neural progenitors (derived from human iPSCs) on aligned poly-caprolactone (PCL) nanofibers.
  
  
  METHODS
  Aligned poly-caprolactone nanofibrous scaffold was fabricated by electrospinning and characterized by scanning electron microscopy (SEM). Through neural induction, neural progenitor cells were derived from induced pluripotent stem cells. After cell seeding on the scaffolds, their proliferation was investigated on different days of culture.
  
  
  RESULTS
  According to the SEM micrographs, the electrospun PCL scaffolds were aligned along with uniformed morphology. Evaluation of adhesion and viability of neural progenitor cells on plate (control) and PCL scaffold illustrated increasing trends in proliferation but this rate was higher in scaffold group. The statistical analyses confirmed significant differences between groups on 36h and 48h.
  
  
  CONCLUSIONS
  Evaluation of cell proliferation along with morphological assessments, staining and SEM finding suggested biocompatibility of the PCL scaffolds and suitability of the combination of the mentioned scaffold and human iPS cells for neural regeneration.
authors.affiliation: Department of developmental biology, faculty of biological science, Kharazmi University, Tehran, Iran ; Stem Cell Biology Department, Stem Cell Technology Research Center, Tehran, Iran.
authors.forename: Parvaneh
authors.initials: P
authors.lastname: Havasi
authors.affiliation: Department of Hematology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
authors.forename: Masoud
authors.initials: M
authors.lastname: Soleimani
authors.affiliation: Department of Histology, School of Veterinary Medicine, Tehran University Tehran, Iran.
authors.forename: Hassan
authors.initials: H
authors.lastname: Morovvati
authors.affiliation: Department of Biotechnology, College of Science, University of Tehran, Tehran, Iran.
authors.forename: Behnaz
authors.initials: B
authors.lastname: Bakhshandeh
authors.affiliation: Department of Developmental Biology, Faculty of Biological Science, Kharazmi University, Tehran, Iran.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Nabiuni
keywords: HipS Cells; Neuronal Progenitors; Poly-Caprolactone Scaffold; Proliferation Assay