title: Diabetes promotes DMH-induced colorectal cancer by increasing the activity of glycolytic enzymes in rats.
abstract: The objective of the present study was to investigate the association between diabetes mellitus and colorectal carcinogenesis as well as the possible mechanism involved in this interaction. Diabetes rat models were induced with a low dose of STZ followed by a low dose of DMH to induce colorectal cancer. The formation of ACF in the colon and the incidence, number and size of tumors were measured. The activity of glycolytic enzymes in colonic tissues was also measured. The results demonstrated that both the total number of ACF and the number of foci that contain a different number of crypts were increased in diabetic rats. At the end of the experimental treatment, the incidence, number and size of tumors were also increased in diabetic rats. Overall, these data indicated that diabetes increased the risk of colorectal cancer. The activity of HK and PK in colonic tissues was increased in diabetic rats, whereas the activity of PDH was decreased. In addition, the activities of these enzymes in intratumor were higher than that of in peritumor. These data indicated that the high rate of glycolysis may play a role in colorectal carcinogenesis in diabetic rats.
authors.affiliation: Department of Biochemistry and Molecular Biology, Shandong University School of Medicine, Jinan, Shandong, China.
authors.forename: Yanglei
authors.initials: Y
authors.lastname: Jia
authors.affiliation: Department of Gastroenterology, 456 Hospital of PLA, Jinan, Shandong, China.
authors.forename: Gang
authors.initials: G
authors.lastname: Xu
authors.affiliation: Department of Biochemistry and Molecular Biology, Shandong University School of Medicine, Jinan, Shandong, China.
authors.forename: Wenjing
authors.initials: W
authors.lastname: Zhou
authors.affiliation: Department of Pathology, 456 Hospital of PLA, Jinan, Shandong, China.
authors.forename: Zhenzheng
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Department of Biochemistry and Molecular Biology, Shandong University School of Medicine, Jinan, Shandong, China.
authors.forename: Linlin
authors.initials: L
authors.lastname: Meng
authors.affiliation: Department of Biochemistry and Molecular Biology, Shandong University School of Medicine, Jinan, Shandong, China.
authors.forename: Songnan
authors.initials: S
authors.lastname: Zhou
authors.affiliation: Department of Biochemistry and Molecular Biology, Shandong University School of Medicine, Jinan, Shandong, China.
authors.forename: Xia
authors.initials: X
authors.lastname: Xu
authors.affiliation: Department of Biochemistry and Molecular Biology, Shandong University School of Medicine, Jinan, Shandong, China.
authors.forename: Huiqing
authors.initials: H
authors.lastname: Yuan
authors.affiliation: Department of Biochemistry and Molecular Biology, Shandong University School of Medicine, Jinan, Shandong, China.
authors.forename: Keli
authors.initials: K
authors.lastname: Tian
mesh_terms: D058739:Aberrant Crypt Foci / Q000473:pathology*; D000818:Animals; D015179:Colorectal Neoplasms / Q000201:enzymology / Q000209:etiology* / Q000473:pathology; D003921:Diabetes Mellitus, Experimental / Q000139:chemically induced* / Q000150:complications*; D006593:Hexokinase / Q000378:metabolism; D006652:Histological Techniques; D006834:Hydrazines / Q000633:toxicity; D008297:Male; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism; D000081382:Pyruvate Dehydrogenase Acetyl-Transferring Kinase; D011770:Pyruvate Kinase / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013438:Sulfhydryl Compounds / Q000633:toxicity
chemical_list: D006834:Hydrazines; C095302:N,N'-dimethyl-N,N'-bis(mercaptoacetyl)hydrazine; D018809:Proliferating Cell Nuclear Antigen; D000081382:Pyruvate Dehydrogenase Acetyl-Transferring Kinase; D013438:Sulfhydryl Compounds; D006593:Hexokinase; D011770:Pyruvate Kinase; D017346:Protein Serine-Threonine Kinases