title: Multicomponent polymeric nanoparticles enhancing intracellular drug release in cancer cells.
abstract: Three kinds of amphiphilic copolymer, that is, poly(ε-caprolactone)-SS-poly(ethylene glycol) (PCL-SS-PEG), poly(ε-caprolactone)-polyethylenimine (PCL-PEI), and poly(ε-caprolactone)-polyethylenimine-folate (PCL-PEI-Fol) were synthesized and self-assembled into surface engineered hybrid nanoparticles (NPs). Morphological studies elucidated the stable, spherical, and uniform sandwich structure of the NPs. PCL-PEI and PCL-SS-PEG segments have introduced pH and reduction responsive characteristics in these NPs, while PCL-PEI-FA copolymers could provide specific targeting capability to cancer cells. The stimuli responsive capabilities of these NPs were carried out. Negative-to-positive charge reversible property, in response to the pH change, was investigated by zeta potential and nuclear magnetic resonance (NMR) measurements. The structure cleavage, due to redox gradient, was studied by dynamic light scattering (DLS) and transmission electron microscopy (TEM). These NPs showed controlled degradation, better drug release, less toxicity, and effective uptake in MCF-7 breast cancer cells. These multifunctional NPs showed promising potential in the treatment of cancer.
authors.affiliation: Department of Radiology, Drum Tower Hospital, School of Medicine, Nanjing University , Nanjing, Jiangsu, P. R. China 210093.
authors.forename: Arsalan
authors.initials: A
authors.lastname: Ahmed
authors.forename: Sen
authors.initials: S
authors.lastname: Liu
authors.forename: Yutong
authors.initials: Y
authors.lastname: Pan
authors.forename: Shanmei
authors.initials: S
authors.lastname: Yuan
authors.forename: Jian
authors.initials: J
authors.lastname: He
authors.forename: Yong
authors.initials: Y
authors.lastname: Hu
mesh_terms: D002208:Caproates / Q000737:chemistry; D045744:Cell Line, Tumor; D003593:Cytoplasm / Q000187:drug effects; D065546:Drug Liberation; D005027:Ethylene Oxide / Q000737:chemistry; D006801:Humans; D007783:Lactones / Q000737:chemistry; D061986:MCF-7 Cells; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles / Q000008:administration & dosage* / Q000737:chemistry; D009369:Neoplasms / Q000188:drug therapy*; D011092:Polyethylene Glycols / Q000737:chemistry; D011094:Polyethyleneimine / Q000737:chemistry
chemical_list: D002208:Caproates; D007783:Lactones; C400421:PLC(20)-b-PEO(44); D011092:Polyethylene Glycols; C121056:caprolactone; D011094:Polyethyleneimine; D005027:Ethylene Oxide
keywords: charge reversibility; nanoparticles; poly(ε-caprolactone); surface engineered; targeting