title: [Plants regeneration from genetically transformed root and callus cultures of periwinkle Vinca minor L. and foxglove purple Digitalis purpurea L].
abstract: Plants regenerated from hairy roots and calluses of foxglove purple and periwinkle have been obtained. It was found that organogenesis in hairy root culture occurs spontaneously on hormone-free medium but with different efficiencies. The frequency of direct shoot formation from root cultures was up to 60% in Digitalis and 3.7% in Vinca. Addition of 1 mg/l BA, 0.1 mg/l NAA and 5% sucrose to B5 medium increased regenerative capacity of Vinca roots up to 19.1%. Regenerated plants showed morphological features typically seen in Ri-transgenic plants. They include growth and plagiotropism of the root system, increased shoot formation, changed leaf morphology and short internodes.
authors.forename: L G
authors.initials: LG
authors.lastname: Leshina
authors.forename: O V
authors.initials: OV
authors.lastname: Bulko
mesh_terms: D003470:Culture Media; D046508:Culture Techniques; D004070:Digitalis / Q000166:cytology / Q000235:genetics* / Q000254:growth & development; D018515:Plant Leaves / Q000235:genetics / Q000254:growth & development; D018517:Plant Roots / Q000166:cytology / Q000235:genetics* / Q000254:growth & development; D018520:Plant Shoots / Q000235:genetics / Q000254:growth & development; D030821:Plants, Genetically Modified / Q000235:genetics* / Q000254:growth & development*; D014170:Transformation, Genetic; D031053:Vinca / Q000166:cytology / Q000235:genetics* / Q000254:growth & development
chemical_list: D003470:Culture Media