title: Detection of avian-like rotavirus A VP4 from a calf in Japan.
abstract: A total of 568 normal feces from calves on a beef farm in Fukui Prefecture, Japan, in 2011-2012 were examined by RT-semi-nested PCR for rotavirus A (RVA) VP4 genes. Through partial sequencing and BLAST analyses of 84 VP4-positive specimens, we identified an avian-like RVA strain, N2342, which shares highest nucleotide identity (80.0%) with known avian-like bovine strain 993/83, in one specimen. Phylogenetic analysis also revealed a close genetic relationship between N2342 and avian RVAs, suggesting bird-to-cattle transmission. We observed frequent contact of wild birds with calves in the farm, suggesting that these birds were the source of the virus.
authors.affiliation: The United Graduate School of Veterinary Sciences, Gifu University, 1-1 Yanagido, Gifu, Gifu 501-1193, Japan.
authors.forename: Hiromichi
authors.initials: H
authors.lastname: Mitake
authors.forename: Naoto
authors.initials: N
authors.lastname: Ito
authors.forename: Kota
authors.initials: K
authors.lastname: Okadera
authors.forename: Kazuma
authors.initials: K
authors.lastname: Okada
authors.forename: Kento
authors.initials: K
authors.lastname: Nakagawa
authors.forename: Tomomi
authors.initials: T
authors.lastname: Tanaka
authors.forename: Kiyohito
authors.initials: K
authors.lastname: Katsuragi
authors.forename: Kasumi
authors.initials: K
authors.lastname: Kasahara
authors.forename: Toshihide
authors.initials: T
authors.lastname: Nihongi
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Tsunemitsu
authors.forename: Makoto
authors.initials: M
authors.lastname: Sugiyama
mesh_terms: D000818:Animals; D036022:Capsid Proteins / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D002417:Cattle; D002418:Cattle Diseases / Q000821:virology*; D015967:Gene Expression Regulation, Viral / Q000502:physiology; D007564:Japan / Q000453:epidemiology; D010802:Phylogeny; D012401:Rotavirus / Q000145:classification*; D012400:Rotavirus Infections / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology
chemical_list: D036022:Capsid Proteins; C060339:VP4 protein, Rotavirus