title: Chemically diverse polymer microarrays and high throughput surface characterisation: a method for discovery of materials for stem cell culture†Electronic supplementary information (ESI) available. See DOI: 10.1039/c4bm00054dClick here for additional data file.
abstract: Materials discovery provides the opportunity to identify novel materials that are tailored to complex biological environments by using combinatorial mixing of monomers to form large libraries of polymers as micro arrays. The materials discovery approach is predicated on the use of the largest chemical diversity possible, yet previous studies into human pluripotent stem cell (hPSC) response to polymer microarrays have been limited to 20 or so different monomer identities in each study. Here we show that it is possible to print and assess cell adhesion of 141 different monomers in a microarray format. This provides access to the largest chemical space to date, allowing us to meet the regenerative medicine challenge to provide scalable synthetic culture ware. This study identifies new materials suitable for hPSC expansion that could not have been predicted from previous knowledge of cell-material interactions.
authors.affiliation: Laboratory of Biophysics and Surface Analysis , School of Pharmacy , University of Nottingham , Nottingham , NG7 2RD , UK . Email: morgan.alexander@nottingham.ac.uk.
authors.forename: A D
authors.initials: AD
authors.lastname: Celiz
authors.affiliation: Wolfson Centre for Stem Cells , Tissue Engineering and Modelling Centre for Biomolecular Sciences , University of Nottingham , Nottingham , NG7 2RD , UK.
authors.forename: J G W
authors.initials: JG
authors.lastname: Smith
authors.affiliation: Wolfson Centre for Stem Cells , Tissue Engineering and Modelling Centre for Biomolecular Sciences , University of Nottingham , Nottingham , NG7 2RD , UK.
authors.forename: A K
authors.initials: AK
authors.lastname: Patel
authors.affiliation: Department of Chemical Engineering , Harvard-MIT Division of Health Sciences and Technology , David H. Koch Institute for Integrative Cancer Research , Massachusetts Institute of Technology , 500 Main Street , Cambridge , MA 02139 , USA.
authors.forename: R
authors.initials: R
authors.lastname: Langer
authors.affiliation: Department of Chemical Engineering , Harvard-MIT Division of Health Sciences and Technology , David H. Koch Institute for Integrative Cancer Research , Massachusetts Institute of Technology , 500 Main Street , Cambridge , MA 02139 , USA.
authors.forename: D G
authors.initials: DG
authors.lastname: Anderson
authors.affiliation: School of Pharmacy , University of Nottingham , Nottingham , NG7 2RD , UK.
authors.forename: D A
authors.initials: DA
authors.lastname: Barrett
authors.affiliation: Wolfson Centre for Stem Cells , Tissue Engineering and Modelling Centre for Biomolecular Sciences , University of Nottingham , Nottingham , NG7 2RD , UK.
authors.forename: L E
authors.initials: LE
authors.lastname: Young
authors.affiliation: Laboratory of Biophysics and Surface Analysis , School of Pharmacy , University of Nottingham , Nottingham , NG7 2RD , UK . Email: morgan.alexander@nottingham.ac.uk.
authors.forename: M C
authors.initials: MC
authors.lastname: Davies
authors.affiliation: Wolfson Centre for Stem Cells , Tissue Engineering and Modelling Centre for Biomolecular Sciences , University of Nottingham , Nottingham , NG7 2RD , UK.
authors.forename: C
authors.initials: C
authors.lastname: Denning
authors.affiliation: Laboratory of Biophysics and Surface Analysis , School of Pharmacy , University of Nottingham , Nottingham , NG7 2RD , UK . Email: morgan.alexander@nottingham.ac.uk.
authors.forename: M R
authors.initials: MR
authors.lastname: Alexander