title: Fibroblast growth factor 21 enhances cholesterol efflux in THP-1 macrophage-derived foam cells.
abstract: Fibroblast growth factor 21 (FGF21) is a novel metabolic regulator. The present study aimed to investigate the effect of FGF21 on cholesterol efflux and the expression of ATP binding cassette (ABC) A1 and G1 in human THP-1 macrophage-derived foam cells. Furthermore, the present study aimed to investigate the role of the liver X receptor (LXR) α in this process. A model of oxidized low-density lipoprotein-induced foam cells from human THP-1 cells was established. The effect of FGF21 on cholesterol efflux was analyzed using a liquid scintillation counter. The expression of ABCA1 and ABCG1 was determined using quantitative polymerase chain reaction and western blot analyses. FGF21 was found to enhance apolipoprotein A1- and high-density lipoprotein-mediated cholesterol efflux. FGF21 was also observed to increase the mRNA and protein expression of ABCA1 and ABCG1. Furthermore, LXRα-short interfering RNA attenuated the stimulatory effects induced by FGF21. These findings suggest that FGF21 may have a protective effect against atherosclerosis by enhancing cholesterol efﬂux through the induction of LXRα-dependent ABCA1 and ABCG1 expression.
authors.affiliation: Department of Cardiology, The First Affiliated Hospital, Chongqing Medical University, Chongqing 400016, P.R. China.
authors.forename: Wei
authors.initials: W
authors.lastname: Shang
authors.affiliation: Department of Cardiology, The First Affiliated Hospital, Chongqing Medical University, Chongqing 400016, P.R. China.
authors.forename: Xuejing
authors.initials: X
authors.lastname: Yu
authors.affiliation: Core Facility of Genetically Engineered Mice, State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P.R. China.
authors.forename: Honglian
authors.initials: H
authors.lastname: Wang
authors.affiliation: Core Facility of Genetically Engineered Mice, State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P.R. China.
authors.forename: Tielin
authors.initials: T
authors.lastname: Chen
authors.affiliation: Core Facility of Genetically Engineered Mice, State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P.R. China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Fang
authors.affiliation: The Division of Molecular Nephrology, The College of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, P.R. China.
authors.forename: Xianggui
authors.initials: X
authors.lastname: Yang
authors.affiliation: The Division of Molecular Nephrology, The College of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, P.R. China.
authors.forename: Puhui
authors.initials: P
authors.lastname: Zhou
authors.affiliation: The Division of Molecular Nephrology, The College of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, P.R. China.
authors.forename: Fang
authors.initials: F
authors.lastname: Nie
authors.affiliation: Core Facility of Genetically Engineered Mice, State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P.R. China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Zhou
authors.affiliation: Department of Cardiology, The First Affiliated Hospital, Chongqing Medical University, Chongqing 400016, P.R. China.
authors.forename: Jianzhong
authors.initials: J
authors.lastname: Zhou
mesh_terms: D064286:ATP Binding Cassette Transporter 1 / Q000235:genetics / Q000378:metabolism; D000070998:ATP Binding Cassette Transporter, Subfamily G, Member 1; D018528:ATP-Binding Cassette Transporters / Q000235:genetics / Q000378:metabolism; D000818:Animals; D016632:Apolipoprotein A-I / Q000378:metabolism; D002460:Cell Line; D002784:Cholesterol / Q000378:metabolism*; D005260:Female; D005346:Fibroblast Growth Factors / Q000494:pharmacology*; D005487:Foam Cells / Q000187:drug effects* / Q000378:metabolism*; D006801:Humans; D000071518:Liver X Receptors; D051379:Mice; D057093:Orphan Nuclear Receptors / Q000378:metabolism; D015854:Up-Regulation
chemical_list: C419353:ABCG1 protein, human; D064286:ATP Binding Cassette Transporter 1; D000070998:ATP Binding Cassette Transporter, Subfamily G, Member 1; D018528:ATP-Binding Cassette Transporters; D016632:Apolipoprotein A-I; D000071518:Liver X Receptors; C000605213:NR1H3 protein, human; C000605214:Nr1h3 protein, mouse; D057093:Orphan Nuclear Receptors; C414620:fibroblast growth factor 21; D005346:Fibroblast Growth Factors; D002784:Cholesterol