title: The sequencing of the complete genome of a Tomato black ring virus (TBRV) and of the RNA2 of three Grapevine chrome mosaic virus (GCMV) isolates from grapevine reveals the possible recombinant origin of GCMV.
abstract: The complete genome of a Tomato black ring virus isolate (TBRV-Mirs) (RNA1, 7,366 nt and RNA2, 4,640 nt) and the RNA2 sequences (4,437; 4,445; and 4,442 nts) of three Grapevine chrome mosaic virus isolates (GCMV-H6, -H15, and -H27) were determined. All RNAs contained a single open reading frame encoding polyproteins of 254 kDa (p1) and 149 kDa (p2) for TBRV-Mirs RNA1 and RNA2, respectively, and 146 kDa for GCMV RNA2. p1 of TBRV-Mirs showed the highest identity with TBRV-MJ (94 %), Beet ringspot virus (BRSV, 82 %), and Grapevine Anatolian ringspot virus (GARSV, 66 %), while p2 showed the highest identity with TBRV isolates MJ (89 %) and ED (85 %), followed by BRSV (65 %), GCMV (58 %), and GARSV (57 %). The amino acid identity of RNA2 sequences of four GCMV isolates (three from this study and one from GenBank) ranged from 91 to 98 %, the homing protein being the most variable. The RDP3 program predicted putative intra-species recombination events for GCMV-H6 and recognized GCMV as a putative inter-species recombinant between GARSV and TBRV. In both cases, the recombination events were at the movement protein level.
authors.affiliation: Istituto Agronomico Mediterraneo di Bari, Via Ceglie 9, 70010, Valenzano, Bari, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Digiaro
authors.forename: E
authors.initials: E
authors.lastname: Yahyaoui
authors.forename: G P
authors.initials: GP
authors.lastname: Martelli
authors.forename: T
authors.initials: T
authors.lastname: Elbeaino
mesh_terms: D016000:Cluster Analysis; D016679:Genome, Viral; D018551:Solanum lycopersicum / Q000821:virology; D008969:Molecular Sequence Data; D008970:Molecular Weight; D017860:Nepovirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D016366:Open Reading Frames; D010802:Phylogeny; D020815:Polyproteins / Q000737:chemistry / Q000235:genetics; D012367:RNA, Viral / Q000235:genetics*; D011995:Recombination, Genetic; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D027843:Vitis / Q000821:virology
chemical_list: D020815:Polyproteins; D012367:RNA, Viral