title: Serum metabolomics study and eicosanoid analysis of childhood atopic dermatitis based on liquid chromatography-mass spectrometry.
abstract: Atopic dermatitis (AD) is the most common inflammatory skin disease in children. In the study, ultra high performance liquid chromatography-mass spectrometry was used to investigate serum metabolic abnormalities of AD children. Two batch fasting sera were collected from AD children and healthy control; one of them was for nontargeted metabolomics analysis, the other for targeted eicosanoids analysis. AD children were divided into high immunoglobulin E (IgE) group and normal IgE group. On the basis of the two analysis approaches, it was found that the differential metabolites of AD, leukotriene B4, prostaglandins, conjugated bile acids, etc., were associated with inflammatory response and bile acids metabolism. Carnitines, free fatty acids, lactic acid, etc., increased in the AD group with high IgE, which revealed energy metabolism disorder. Amino acid metabolic abnormalities and increased levels of Cytochrome P450 epoxygenase metabolites were found in the AD group with normal IgE. The results provided a new perspective to understand the mechanism and find potential biomarkers of AD and may provide a new reference for personalized treatment.
authors.affiliation: Dalian Children's Hospital , Dalian 116011, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Huang
authors.forename: Guoyou
authors.initials: G
authors.lastname: Chen
authors.forename: Xinyu
authors.initials: X
authors.lastname: Liu
authors.forename: Yaping
authors.initials: Y
authors.lastname: Shao
authors.forename: Peng
authors.initials: P
authors.lastname: Gao
authors.forename: Chenchen
authors.initials: C
authors.lastname: Xin
authors.forename: Zhenze
authors.initials: Z
authors.lastname: Cui
authors.forename: Xinjie
authors.initials: X
authors.lastname: Zhao
authors.forename: Guowang
authors.initials: G
authors.lastname: Xu
mesh_terms: D001647:Bile Acids and Salts / Q000097:blood; D015415:Biomarkers / Q000097:blood; D002331:Carnitine / Q000097:blood; D002675:Child, Preschool; D002853:Chromatography, Liquid / Q000379:methods*; D003876:Dermatitis, Atopic / Q000097:blood / Q000175:diagnosis / Q000378:metabolism*; D015777:Eicosanoids / Q000097:blood / Q000378:metabolism*; D005230:Fatty Acids, Nonesterified / Q000097:blood; D005260:Female; D006801:Humans; D007073:Immunoglobulin E / Q000097:blood; D007223:Infant; D019344:Lactic Acid / Q000097:blood; D007975:Leukotriene B4 / Q000097:blood; D008297:Male; D013058:Mass Spectrometry / Q000379:methods*; D055442:Metabolome; D055432:Metabolomics / Q000379:methods*; D011453:Prostaglandins / Q000097:blood; D012372:ROC Curve; D015203:Reproducibility of Results
chemical_list: D001647:Bile Acids and Salts; D015415:Biomarkers; D015777:Eicosanoids; D005230:Fatty Acids, Nonesterified; D011453:Prostaglandins; D007975:Leukotriene B4; D019344:Lactic Acid; D007073:Immunoglobulin E; D002331:Carnitine
keywords: Atopic dermatitis; LC−MS,; eicosanoids; immunoglobulin E; metabolomics