title: Paramagnetic cellulose DNA isolation improves DNA yield and quality among diverse plant taxa.
abstract: OBJECTIVE
  The chemical diversity of land plants ensures that no single DNA isolation method results in high yield and purity with little effort for all species. Here we evaluate a new technique originally developed for forensic science, based on MagnaCel paramagnetic cellulose particles (PMC), to determine its efficacy in extracting DNA from 25 plant species representing 21 families and 15 orders. •
  
  
  RESULTS
  Yield and purity of DNA isolated by PMC, DNeasy Plant Mini Kit (silica column), and cetyltrimethylammonium bromide (CTAB) methods were compared among four individuals for each of 25 plant species. PMC gave a twofold advantage in average yield, and the relative advantage of the PMC method was greatest for samples with the lowest DNA yields. PMC also produced more consistent sample purity based on absorbance ratios at 260:280 and 260:230 nm. •
  
  
  CONCLUSIONS
  PMC technology is a promising alternative for plant DNA isolation.
authors.affiliation: Department of Botany, University of Wisconsin-Madison, 430 Lincoln Drive, Madison, Wisconsin 53706 USA.
authors.forename: Jackson R
authors.initials: JR
authors.lastname: Moeller
authors.affiliation: Department of Botany, University of Wisconsin-Madison, 430 Lincoln Drive, Madison, Wisconsin 53706 USA.
authors.forename: Nicholas R
authors.initials: NR
authors.lastname: Moehn
authors.affiliation: Department of Botany, University of Wisconsin-Madison, 430 Lincoln Drive, Madison, Wisconsin 53706 USA.
authors.forename: Donald M
authors.initials: DM
authors.lastname: Waller
authors.affiliation: Department of Botany, University of Wisconsin-Madison, 430 Lincoln Drive, Madison, Wisconsin 53706 USA.
authors.forename: Thomas J
authors.initials: TJ
authors.lastname: Givnish
keywords: DNA isolation; DNeasy; cetyltrimethylammonium bromide (CTAB); paramagnetic cellulose particles (PMC); silica columns