title: Preparation of chemically modified RNA origami nanostructures.
abstract: In nucleic acid nanotechnology, designed RNA molecules are widely explored because of their usability originating from RNA's structural and functional diversity. Herein, a method to design and prepare RNA nanostructures by employing DNA origami strategy was developed. A single-stranded RNA scaffold and staple RNA strands were used for the formation of RNA nanostructures. After the annealing of the mixtures, 7-helix bundled RNA tile and 6-helix bundled RNA tube structures were observed as predesigned shapes. These nanostructures were easily functionalized by introducing chemical modification to the RNA scaffolds. The DNA origami method is extended and utilized to construct RNA nanostructures.
authors.affiliation: Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University, Yoshida-ushinomiyacho, Sakyo-ku, Kyoto 606-8501 (Japan); CREST (Japan) Science and Technology Agency (JST), Sanbancho, Chiyoda-ku, Tokyo 102-0075 (Japan). endo@kuchem.kyoto-u.ac.jp.
authors.forename: Masayuki
authors.initials: M
authors.lastname: Endo
authors.forename: Yosuke
authors.initials: Y
authors.lastname: Takeuchi
authors.forename: Tomoko
authors.initials: T
authors.lastname: Emura
authors.forename: Kumi
authors.initials: K
authors.lastname: Hidaka
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Sugiyama
mesh_terms: D004591:Electrophoresis, Polyacrylamide Gel; D018625:Microscopy, Atomic Force; D049329:Nanostructures / Q000737:chemistry*; D009690:Nucleic Acid Conformation; D012313:RNA / Q000737:chemistry*; D014529:Uridine / Q000737:chemistry
chemical_list: D012313:RNA; D014529:Uridine
keywords: RNA; atomic force microscopy; chemical modification; nanostructures; transcription