title: Alpha B-crystallin correlates with poor survival in colorectal cancer.
abstract: Alpha B-crystallin (CRYAB) is primarily found as major structural proteins of the ocular lens and is a principal member of the small heat shock protein (HSP) family. So far, CRYAB has been suggested to play critical roles in the development of several kinds of human cancers. However, the association between CRYAB expression and clinicopathological characteristics of colorectal cancer (CRC) has not been elucidated yet. In the present study, one-step quantitative PCR reverse transcription-polymerase chain reaction (qPCR) analysis of 18 samples of CRC and immunohistochemistry (IHC) analysis with 100 cases of CRC sample in tissue microarrays (TMA) were employed to evaluate the expression of CRYAB in CRC. The results suggested that CRYAB expression in the mRNA and protein levels was significantly higher in CRC tissues than in corresponding non-cancerous tissues (P < 0.05 and P = 0.014, respectively). The expression of CRYAB protein in CRC was significantly associated with distant metastasis (P = 0.040) and overall survival (P = 0.003). Kaplan-Meier method and multivariate survival analysis indicated that high expression of CRYAB (P = 0.040) and distant metastasis (P = 0.005) showed significant correlations with poor prognosis of CRC patients. The data imply that CRYAB expression is correlated with substantial clinical characteristics of CRC, and it may be identified as an unfavorable prognostic factor for CRC.
authors.affiliation: Department of Pathology and Pathophysiology, School of Medicine, Southeast University Nanjing 210009, China.
authors.forename: Chuanbing
authors.initials: C
authors.lastname: Shi
authors.affiliation: Department of General Surgery, The Second Affiliated Hospital of Nanjing Medical University Nanjing 210000, China.
authors.forename: Zhenyu
authors.initials: Z
authors.lastname: He
authors.affiliation: Department of Pathology, Jiangsu Cancer Hospital Nanjing 210000, China.
authors.forename: Ning
authors.initials: N
authors.lastname: Hou
authors.affiliation: Department of Traumatic Surgery, Changzhou No. 2 People's Hospital Affiliated with Nanjing Medical University Changzhou 213000, China.
authors.forename: Yijiang
authors.initials: Y
authors.lastname: Ni
authors.affiliation: Department of Pathology, The Second Affiliated Hospital of Nanjing Medical University Nanjing 210000, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Xiong
authors.affiliation: Department of Pathology and Pathophysiology, School of Medicine, Southeast University Nanjing 210009, China.
authors.forename: Pingsheng
authors.initials: P
authors.lastname: Chen
mesh_terms: D014408:Biomarkers, Tumor / Q000032:analysis* / Q000235:genetics; D016009:Chi-Square Distribution; D015179:Colorectal Neoplasms / Q000737:chemistry* / Q000235:genetics / Q000401:mortality / Q000473:pathology / Q000628:therapy; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D053208:Kaplan-Meier Estimate; D008297:Male; D008875:Middle Aged; D015999:Multivariate Analysis; D009367:Neoplasm Staging; D011237:Predictive Value of Tests; D016016:Proportional Hazards Models; D012333:RNA, Messenger / Q000032:analysis; D060888:Real-Time Polymerase Chain Reaction; D012189:Retrospective Studies; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012307:Risk Factors; D013997:Time Factors; D046888:Tissue Array Analysis; D015854:Up-Regulation; D038203:alpha-Crystallin B Chain / Q000032:analysis* / Q000235:genetics
chemical_list: D014408:Biomarkers, Tumor; C059215:CRYAB protein, human; D012333:RNA, Messenger; D038203:alpha-Crystallin B Chain
keywords: CRC; CRYAB; IHC; TMA; qPCR