title: Statistical Optimization of Medium Components for Mass Production of Plant Growth-Promoting Microbial Inoculant Pseudomonas trivialis BIHB 745 (MTCC5336).
abstract: Optimizing nutritional requirements for mass production of microbial inoculants in shortened time has relevance for their economical field application. Therefore, the present study aimed at selecting suitable growth medium, optimizing its components, and up-scaling inoculum production for plant growth-promoting Pseudomonas trivialis BIHB 745. Of the different media tested, the culture exhibited maximal viable colony count in trypticase soya broth with 17.6 % increased biomass on optimizing levels of carbon source, nitrogen source, and NaCl using response surface methodology. A twofold higher biomass with 9 h shorter incubation period was obtained in optimized medium in a bioreactor in comparison to shake flasks.
authors.affiliation: Plant Pathology and Microbiology Laboratory, CSIR-Institute of Himalayan Bioresource Technology, P.O. Box No. 6, Palampur, 176 061 Himachal Pradesh India.
authors.forename: Pratibha
authors.initials: P
authors.lastname: Vyas
authors.affiliation: Plant Pathology and Microbiology Laboratory, CSIR-Institute of Himalayan Bioresource Technology, P.O. Box No. 6, Palampur, 176 061 Himachal Pradesh India.
authors.forename: Praveen
authors.initials: P
authors.lastname: Rahi
authors.affiliation: Microbiology Department, Guru Nanak Dev University, Amritsar, 143 005 Punjab India.
authors.forename: B S
authors.initials: BS
authors.lastname: Chadha
authors.affiliation: Plant Pathology and Microbiology Laboratory, CSIR-Institute of Himalayan Bioresource Technology, P.O. Box No. 6, Palampur, 176 061 Himachal Pradesh India.
authors.forename: Arvind
authors.initials: A
authors.lastname: Gulati
keywords: Medium optimization; Plant growth-promoting bacteria; Pseudomonas trivialis