title: Effects of topical chondrocyte-derived extracellular matrix treatment on corneal wound healing, following an alkali burn injury.
abstract: Numerous treatments have been used in the management of corneal chemical burns; however, no optimal treatment for corneal chemical burns currently exists. The present study investigated the effects of topical chondrocyte-derived extracellular matrix (CD-ECM) treatment on corneal wound healing, using an alkali burn mouse model. Topical treatment with CD-ECM was shown to reduce corneal opacity following an alkali burn. A histological examination observed the presence of regenerated epithelial cells and a small number of inflammatory cells in the corneas of CD-ECM-treated mice. The majority of the inflammatory cells present in the corneas of the phosphate-buffered saline (PBS)-treated mice were neutrophils that expressed matrix metalloproteinase (MMP)-9. The amount of neutrophils was significantly reduced in the corneas of the CD-ECM-treated mice. Furthermore, the expression levels of interleukin (IL)-8 were significantly reduced in the CD-ECM treatment group, but not in the mice that received the PBS treatment. The results of the present study indicate that CD-ECM treatment may accelerate wound healing in a model of alkali burn-induced corneal injury. The therapeutic mechanism may be associated with accelerated reepithelialization and reduced recruitment of MMP-9-expressing neutrophils, through inhibiting the production of IL-8.
authors.affiliation: Department of Ophthalmology, Busan Paik Hospital, Inje University, Busan 614‑735, Republic of Korea.
authors.forename: Jae-Wook
authors.initials: JW
authors.lastname: Yang
authors.affiliation: Ocular Neovascular Research Center, College of Medicine, Inje University, Busan 614‑735, Republic of Korea.
authors.forename: Soung-Min
authors.initials: SM
authors.lastname: Lee
authors.affiliation: Ocular Neovascular Research Center, College of Medicine, Inje University, Busan 614‑735, Republic of Korea.
authors.forename: Kyeong-Hee
authors.initials: KH
authors.lastname: Oh
authors.affiliation: Ocular Neovascular Research Center, College of Medicine, Inje University, Busan 614‑735, Republic of Korea.
authors.forename: Sae-Gwang
authors.initials: SG
authors.lastname: Park
authors.affiliation: Ocular Neovascular Research Center, College of Medicine, Inje University, Busan 614‑735, Republic of Korea.
authors.forename: Ii-Whan
authors.initials: IW
authors.lastname: Choi
authors.affiliation: Ocular Neovascular Research Center, College of Medicine, Inje University, Busan 614‑735, Republic of Korea.
authors.forename: Su-Kil
authors.initials: SK
authors.lastname: Seo
mesh_terms: D000287:Administration, Topical; D000818:Animals; D002057:Burns, Chemical; D019902:Chondrocytes; D065306:Corneal Injuries / Q000139:chemically induced* / Q000188:drug therapy; D004195:Disease Models, Animal; D005109:Extracellular Matrix; D005126:Eye Burns / Q000139:chemically induced* / Q000188:drug therapy; D005260:Female; D016209:Interleukin-8 / Q000096:biosynthesis; D020780:Matrix Metalloproteinase 9; D051379:Mice; D020556:Neutrophil Infiltration; D009504:Neutrophils / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D016209:Interleukin-8; D020780:Matrix Metalloproteinase 9