title: Preparation and application of collagen scaffold-encapsulated silver nanoparticles and bone morphogenetic protein 2 for enhancing the repair of infected bone.
abstract: The healing of contaminated/infected bone defects is a significant clinical challenge. Here, a novel collagen scaffold composite encapsulating silver nanoparticles (AgNP) and bone morphogenetic protein 2 (BMP-2) was prepared to enhance the healing of infected bone defects. Collagen scaffolds conjugated with AgNP possessed strong antibacterial properties that were dependent on the release rate of Ag(+). After introducing BMP-2, the BMP-2/AgNP/collagen scaffold composites did not adversely affect the adherence or proliferation of bone marrow-derived mesenchymal stromal cells (BMSCs). Differentiation of BMSCs toward osteoblasts was induced by the upregulation of RUNX2, osteopontin and osteonectin expression. BMP-2/AgNP/collagen scaffold composites, therefore, possess the antibacterial activity of AgNP and the osteoinductivity of BMP-2, making these composites an ideal pharmaceutical for the regeneration of bone in infected wounds.
authors.affiliation: Department of Orthopedics, Peace Hospital Affiliated to Changzhi Medical College, Changzhi, 046000, Shanxi, China.
authors.forename: Chang-Ying
authors.initials: CY
authors.lastname: Sun
authors.forename: Yan-Jun
authors.initials: YJ
authors.lastname: Che
authors.forename: Shi-Jin
authors.initials: SJ
authors.lastname: Lu
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000494:pharmacology; D055396:Bone Morphogenetic Protein 2 / Q000737:chemistry* / Q000494:pharmacology; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D003094:Collagen / Q000737:chemistry*; D059630:Mesenchymal Stem Cells; D053768:Metal Nanoparticles / Q000737:chemistry*; D010012:Osteogenesis / Q000187:drug effects; D011817:Rabbits; D012834:Silver / Q000737:chemistry* / Q000493:pharmacokinetics / Q000494:pharmacology; D013211:Staphylococcus aureus / Q000187:drug effects; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D000900:Anti-Bacterial Agents; D055396:Bone Morphogenetic Protein 2; D012834:Silver; D003094:Collagen