title: Levels of Selected Aqueous Humor Mediators (IL-10, IL-17, CCL2, VEGF, FasL) in Diabetic Cataract.
abstract: OBJECTIVE
  To compare levels of selected mediators in serums and aqueous humor (AH) of type 2 diabetes mellitus cataract patients with senile cataract patients, and to determine their association with postoperative corneal edema (CE).
  
  
  METHODS
  Patients (32 senile and 29 diabetic cataract) undergoing standardized phacoemulsification combined with intraocular lens implantation were recruited. CE was assessed using an ordinal scale (grade 0 to 3). IL-10, CCL2, IL-17, FasL, and VEGF were measured by ELISA.
  
  
  RESULTS
  Diabetic patients had higher AH levels of VEGF (p = .042) and IL-10 (p = .021), lower AH levels of FasL (p = .048), and higher serum levels of CCL2 (p = .002). AH levels of CCL2 were higher in diabetic patients with more severe CE at the first postoperative day (p = .012).
  
  
  CONCLUSIONS
  We found disturbed AH microenvironment in diabetic cataract, with significant changes for VEGF, IL-10, and FasL. Higher CCL2 was associated with the development of early postoperative CE in diabetic patients.
authors.affiliation: a Department of Ophthalmology , General Hospital, "Dr. J. Benčević," Slavonski Brod, Croatia; and Ophthalmology Clinic , Slavonski Brod , Croatia and.
authors.forename: Sanja
authors.initials: S
authors.lastname: Mitrović
authors.affiliation: b Department of Physiology and Immunology and Laboratory for Molecular Immunology , University of Zagreb School of Medicine , Zagreb , Croatia.
authors.forename: Tomislav
authors.initials: T
authors.lastname: Kelava
authors.affiliation: b Department of Physiology and Immunology and Laboratory for Molecular Immunology , University of Zagreb School of Medicine , Zagreb , Croatia.
authors.forename: Alan
authors.initials: A
authors.lastname: Šućur
authors.affiliation: b Department of Physiology and Immunology and Laboratory for Molecular Immunology , University of Zagreb School of Medicine , Zagreb , Croatia.
authors.forename: Danka
authors.initials: D
authors.lastname: Grčević
mesh_terms: D000368:Aged; D001082:Aqueous Humor / Q000378:metabolism*; D015415:Biomarkers / Q000097:blood / Q000378:metabolism*; D018916:Blood-Aqueous Barrier; D002386:Cataract / Q000097:blood / Q000378:metabolism*; D018932:Chemokine CCL2 / Q000097:blood / Q000378:metabolism; D003924:Diabetes Mellitus, Type 2 / Q000097:blood / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D005136:Eye Proteins / Q000097:blood / Q000378:metabolism*; D053222:Fas Ligand Protein / Q000097:blood / Q000378:metabolism; D005260:Female; D006801:Humans; D016753:Interleukin-10 / Q000097:blood / Q000378:metabolism; D020381:Interleukin-17 / Q000097:blood / Q000378:metabolism; D019654:Lens Implantation, Intraocular; D008297:Male; D018918:Phacoemulsification; D042461:Vascular Endothelial Growth Factor A / Q000097:blood / Q000378:metabolism
chemical_list: D015415:Biomarkers; C111835:CCL2 protein, human; D018932:Chemokine CCL2; D005136:Eye Proteins; C505661:FASLG protein, human; D053222:Fas Ligand Protein; C508609:IL10 protein, human; D020381:Interleukin-17; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A; D016753:Interleukin-10
keywords: Aqueous humor; cataract; chemokines; cytokines; type 2 diabetes mellitus