title: Restoration of miR-7 expression suppresses the growth of Lewis lung cancer cells by modulating epidermal growth factor receptor signaling.
abstract: microRNAs are an abundant class of short endogenous non-coding RNAs that function as important regulators of multiple target genes and participate in diverse biological roles in carcinogenesis. However, the role of miR-7 in lung cancer remains unclear and requires further elucidation. In the present study, we found a reduction of miR-7 expression in Lewis lung cancer (3LL) cells originating from mice by real-time RT-PCR. Restoration of miR-7 inhibited 3LL cell proliferation, induced cell apoptosis in vitro and reduced tumorigenicity in vivo. We further confirmed that miR-7 downregulated the expression of both epidermal growth factor receptor (EGFR) and murine leukemia viral oncogene homologue-1 (RAF-1) oncogenes by real-time PCR and western blot analysis. Furthermore, inhibition of EGFR showed similar effects to miR-7 enforcement in 3LL cells. Taken together, these findings revealed that miR-7 acts as an antitumor miRNA in 3LL by targeting and suppressing the expression of both EGFR and RAF-1 oncogenes. This study may provide a rationale for the use of miR-7 in lung cancer target therapy.
authors.affiliation: Department of Emergency, The Second Hospital of Anhui Medical University, Hefei, Anhui, P.R. China.
authors.forename: Jingrong
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Medicine, College of Physicians and Surgeons, Columbia University, New York, NY, USA.
authors.forename: Yijie
authors.initials: Y
authors.lastname: Zheng
authors.affiliation: Department of Respiratory Medicine, The First Affiliated Hospital of Anhui Medical University, Hefei, P.R. China.
authors.forename: Gengyun
authors.initials: G
authors.lastname: Sun
authors.affiliation: Department of Hematology/Oncology, The Second Hospital of Anhui Medical University, Hefei, Anhui, P.R. China.
authors.forename: Shudao
authors.initials: S
authors.lastname: Xiong
mesh_terms: D000818:Animals; D017209:Apoptosis; D018827:Carcinoma, Lewis Lung / Q000378:metabolism* / Q000473:pathology; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D066246:ErbB Receptors / Q000235:genetics / Q000378:metabolism*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D008810:Mice, Inbred C57BL; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D019908:Proto-Oncogene Proteins c-raf / Q000235:genetics / Q000378:metabolism; D034622:RNA Interference; D015398:Signal Transduction
chemical_list: C549243:MIRN7 microRNA, mouse; D035683:MicroRNAs; C541485:EGFR protein, mouse; D066246:ErbB Receptors; D019908:Proto-Oncogene Proteins c-raf