title: Serum protein S100A9, SOD3, and MMP9 as new diagnostic biomarkers for pulmonary tuberculosis by iTRAQ-coupled two-dimensional LC-MS/MS.
abstract: This study aimed to discover the novel noninvasive biomarkers for the diagnosis of pulmonary tuberculosis (TB). We applied iTRAQ 2D LC-MS/MS technique to investigate protein profiles in patients with pulmonary TB and other lung diseases. A total of 34 differentially expressed proteins (24 upregulated proteins and ten downregulated proteins) were identified in the serum of pulmonary TB patients. Significant differences in protein S100-A9 (S100A9), extracellular superoxide dismutase [Cu-Zn] (SOD3), and matrix metalloproteinase 9 (MMP9) were found between pulmonary TB and other lung diseases by ELISA. Correlations analysis revealed that the serum concentration of MMP9 in the pulmonary TB was in moderate correlation with SOD3 (r = 0.581) and S100A9 (r = 0.471), while SOD3 was in weak correlation with S100A9 (r = 0.287). The combination of serum S100A9, SOD3, and MMP9 levels could achieve 92.5% sensitivity and 95% specificity to discriminate between pulmonary TB and healthy controls, 90% sensitivity and 87.5% specificity to discriminate between pulmonary TB and pneumonia, and 85% sensitivity and 92.5% specificity to discriminate between pulmonary TB and lung cancer, respectively. The results showed that S100A9, SOD3, and MMP9 may be potential diagnostic biomarkers for pulmonary TB, and provided experimental basis for the diagnosis of pulmonary TB.
authors.affiliation: Institute of Cell Biology, Zhejiang University, Hangzhou, China.
authors.forename: Dandan
authors.initials: D
authors.lastname: Xu
authors.forename: Yanyuan
authors.initials: Y
authors.lastname: Li
authors.forename: Xiang
authors.initials: X
authors.lastname: Li
authors.forename: Li-Liang
authors.initials: LL
authors.lastname: Wei
authors.forename: Zhifen
authors.initials: Z
authors.lastname: Pan
authors.forename: Ting-Ting
authors.initials: TT
authors.lastname: Jiang
authors.forename: Zhong-Liang
authors.initials: ZL
authors.lastname: Chen
authors.forename: Chong
authors.initials: C
authors.lastname: Wang
authors.forename: Wen-Ming
authors.initials: WM
authors.lastname: Cao
authors.forename: Xing
authors.initials: X
authors.lastname: Zhang
authors.forename: Ze-Peng
authors.initials: ZP
authors.lastname: Ping
authors.forename: Chang-Ming
authors.initials: CM
authors.lastname: Liu
authors.forename: Ji-Yan
authors.initials: JY
authors.lastname: Liu
authors.forename: Zhong-Jie
authors.initials: ZJ
authors.lastname: Li
authors.forename: Ji-Cheng
authors.initials: JC
authors.lastname: Li
mesh_terms: D000328:Adult; D000368:Aged; D015415:Biomarkers / Q000097:blood; D040502:Calgranulin B / Q000097:blood*; D002853:Chromatography, Liquid; D005260:Female; D006801:Humans; D008297:Male; D020780:Matrix Metalloproteinase 9 / Q000097:blood*; D008875:Middle Aged; D040901:Proteomics; D013482:Superoxide Dismutase / Q000097:blood*; D053719:Tandem Mass Spectrometry; D014397:Tuberculosis, Pulmonary / Q000097:blood* / Q000175:diagnosis; D055815:Young Adult
chemical_list: D015415:Biomarkers; D040502:Calgranulin B; C441642:SOD3 protein, human; D013482:Superoxide Dismutase; D020780:Matrix Metalloproteinase 9
keywords: Cell biology; MMP9; Potential biomarkers; Pulmonary tuberculosis; S100A9; SOD3