title: Exogenous recombinant adiponectin improves survival in experimental abdominal sepsis.
abstract: BACKGROUND
  Adiponectin, which has anti-inflammatory features, is an important substance in several metabolic mechanisms.
  
  
  OBJECTIVE
  The aim of this study is to evauate the effects of exogenous intraperitoneal administration of adiponectin on the survival, intrabdominal adhesion and inflammatory cytokine levels in an experimental sepsis model.
  
  
  METHODS
  Animal experimentation.
  
  
  METHODS
  Ninety rats were divided into a control group, adiponectin group and sham group. A cecal puncture abdominal sepsis model was performed in the adiponectin and control groups. Every three hours, exogenous adiponectin was administrated to the adiponectin group. At the 3(rd) and 24(th) hours, 10 rats were sacrified in each group in order to measure plasma tumor necrosis factor-α (TNF-α), interleukin (IL) 10, soluble intracellular adhesion molecule (ICAM)-1, IL-6 and macrophage inhibitory factor levels, and the activity of nuclear factor (NF)-kB. The remaining rats were followed for survival.
  
  
  RESULTS
  The plasma levels of TNF-α, soluable ICAM-1, IL-6, and macrophage inhibitory factor were significantly higher in the control group than in the adiponectin and sham group (p<0.05). The increase in inflammatory cytokines with time was more prominent in the control group. The activity of NF-kB in the control group was higher than in the adiponectin group (p<0.05). The survival rate of the adiponectin group was higher than in the control group.
  
  
  CONCLUSIONS
  Administration of exogenous adiponectin to the peritoneum in abdominal sepsis increased survival and decreased intrabdominal adhesions by decreasing the inflammatory response.
authors.affiliation: Department of General Surgery, Gazi University Faculty of Medicine, Ankara, Turkey.
authors.forename: Bülent
authors.initials: B
authors.lastname: Salman
authors.affiliation: Department of General Surgery, Kocaeli Universtiy Faculty of Medicine, Kocaeli, Turkey.
authors.forename: Tonguç Utku
authors.initials: TU
authors.lastname: Yılmaz
authors.affiliation: Department of General Surgery, Başkent University Faculty of Medicine, Ankara, Turkey.
authors.forename: Tugan
authors.initials: T
authors.lastname: Tezcaner
authors.affiliation: Department of Medical Serivices and Techniques, Bülent Ecevit University Vocational School of Health Services, Zonguldak, Turkey.
authors.forename: Ebru Ofluoğlu
authors.initials: EO
authors.lastname: Demir
authors.affiliation: Department of Biochemistry, Gazi University Faculty of Health Sciences, Ankara, Turkey.
authors.forename: Ozge Tuğçe
authors.initials: OT
authors.lastname: Paşaoğlu
keywords: Adhesion; adiponectin; cytokine; sepsis; survival