title: Bilateral vagotomy attenuates the severity of secretagogue-induced acute pancreatitis in the rat.
abstract: OBJECTIVE
  We assessed the effect of bilateral vagotomy (BV) on the course of acute caerulein-induced pancreatitis (AP) in the rat.
  
  
  METHODS
  The study was performed on Wistar rats surgically prepared by subdiaphragmatic BV. Control group underwent sham operation. Four days later, AP was induced by subcutaneous injection of caerulein (25 μg/kg/5h) to the conscious animals with or without BV. After administration of caerulein the blood samples were taken for determination of serum lipase activity and interleukin-10 (IL-10) concentration. Pancreatic tissue samples were subjected to histological examinations and to the measurement of lipid peroxidation products (MDA+4-HNE) concentration and the activity of an antioxidant enzyme - glutathione peroxidase (GPx). After application of caerulein pancreatic blood flow was measured by laser Doppler flowmetry.
  
  
  RESULTS
  AP was manifested by oedema and neutrophil infiltration of the pancreatic tissue and accompanied by significant increases of serum lipase activity, serum concentration of IL-10 and pancreatic concentration of MDA+4HNE (ca. 50×, 2× and 4× respectively p ≥ 0.05). Pancreatic activity of GPx and pancreatic blood flow were decreased (both by 60%). In vagotomised rats with AP serum lipase activity and pancreatic concentration of MDA+4-HNE were lower whereas Il-10 concentration and pancreatic activity of GPx, as well as pancreatic blood flow were significantly higher as compared to AP rats with intact vagal nerves. In AP rats with vagotomy all histological signs of pancreatitis were significantly reduced.
  
  
  CONCLUSIONS
  Bilateral vagotomy resulted in the significant attenuation of caerulein-induced pancreatitis in the rat.
authors.affiliation: Department of Medical Physiology, Health Care Faculty, Jagiellonian University Collegium Medicum, Krakow, Poland.
authors.forename: Joanna
authors.initials: J
authors.lastname: Szklarczyk
authors.affiliation: Department of Medical Physiology, Health Care Faculty, Jagiellonian University Collegium Medicum, Krakow, Poland. Electronic address: mpjawore@cyf-kr.edu.pl.
authors.forename: Jolanta
authors.initials: J
authors.lastname: Jaworek
authors.affiliation: Department of Medical Physiology, Health Care Faculty, Jagiellonian University Collegium Medicum, Krakow, Poland.
authors.forename: Urszula
authors.initials: U
authors.lastname: Czech
authors.affiliation: Department of Medical Physiology, Health Care Faculty, Jagiellonian University Collegium Medicum, Krakow, Poland.
authors.forename: Joanna
authors.initials: J
authors.lastname: Bonior
authors.affiliation: Department of Medical Physiology, Health Care Faculty, Jagiellonian University Collegium Medicum, Krakow, Poland.
authors.forename: Michalina
authors.initials: M
authors.lastname: Kot
authors.affiliation: Department of Cell Morphology Medical Faculty, Jagiellonian University Collegium Medicum, Krakow, Poland.
authors.forename: Romana
authors.initials: R
authors.lastname: Tomaszewska
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000097:blood / Q000378:metabolism; D004195:Disease Models, Animal; D008297:Male; D018384:Oxidative Stress; D010179:Pancreas / Q000098:blood supply* / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D010195:Pancreatitis / Q000276:immunology / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology*; D017208:Rats, Wistar; D012039:Regional Blood Flow; D012720:Severity of Illness Index; D014628:Vagotomy
chemical_list: D015415:Biomarkers
keywords: Bilateral vagotomy; Caerulein-induced pancreatitis; Interleukin 10; Lipase; Oxidative stress; Pancreatic blood flow