title: [The development of reagents set in the format of DNA-chip for genetic typing of strains of Vibrio cholerae].
abstract: The necessity of development of methods of genic diagnostic of cholera is conditioned by continuation of the Seventh pandemic of cholera, taxonomic variability of strains of Vibrio cholerae involved into pandemic and also permanent danger of delivery of disease to the territory of the Russian Federation. The methods of genic diagnostic of cholera make it possible in a comparatively short time to maximally minutely characterize strains isolated from patients or their environment. The article presents information about working out reagents set for genetic typing of agents of cholera using DNA-chip. The makeup of DNA-chip included oligonucleotide probes making possible to differentiate strains of V. cholerae on serogroups and biovars and to determine their pathogenicity. The single DNA-chip makes it possible to genetically type up to 12 samples concurrently. At that, duration of analysis without accounting stage of DNA separation makes up to 5 hours. In the progress of work, 23 cholera and non-cholera strains were analyzed. The full compliance of DNA-chip typing results to previously known characteristics of strains. Hence, there is a reason to consider availability of further development of reagents set and possibility of its further application in laboratories of regional level and reference centers.
authors.forename: E A
authors.initials: EA
authors.lastname: Pudova
authors.forename: M L
authors.initials: ML
authors.lastname: Markelov
authors.forename: V G
authors.initials: VG
authors.lastname: Dedkov
authors.forename: T A
authors.initials: TA
authors.lastname: Tchekanova
authors.forename: A I
authors.initials: AI
authors.lastname: Sadjin
authors.forename: N P
authors.initials: NP
authors.lastname: Kirdiyashkina
authors.forename: M V
authors.initials: MV
authors.lastname: Bekova
authors.forename: A A
authors.initials: AA
authors.lastname: Deviyatkin
mesh_terms: D060005:Genotyping Techniques / Q000379:methods*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D014734:Vibrio cholerae / Q000145:classification / Q000235:genetics*