title: Protective effects of Lingguizhugan decoction on amyloid-beta peptide (25-35)-induced cell injury: Anti-inflammatory effects.
abstract: In the present study, a human neuroblastoma cell line (SH-SY5Y) and BV-2 microglia were treated with amyloid-β peptide (25-35), as a model of Alzheimer's disease, to evaluate the protective effects of 10-3-10-8 g/mL Lingguizhugan decoction and to examine the underlying anti-inflammatory mechanism. Lingguizhugan decoction significantly enhanced the viability of SH-SY5Y cells with amyloid-β peptide-induced injury, and lowered levels of interleukin-1β, interleukin-6, tumor necrosis factor-α and nitric oxide in the culture supernatant of activated BV-2 microglia. The effects of 10-3 g/mL Lingguizhugan decoction were more significant. These results suggest that Lingguizhugan decoction can protect SH-SY5Y cells against amyloid-β peptide (25-35)-induced injury in a dose-dependent manner by inhibiting overexpression of inflammatory factors by activated microglia.
authors.affiliation: Basic Medicine College, Nanjing University of Chinese Medicine, Nanjing 210046, Jiangsu Province, China.
authors.forename: Feifei
authors.initials: F
authors.lastname: Xi
authors.affiliation: Henan Clinical Research Center of AIDS Controlled with Traditional Chinese Medicine, the First Affiliated Hospital of Henan University of Traditional Chinese Medicine, Zhengzhou 450003, Henan Province, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Sang
authors.affiliation: Basic Medicine College, Nanjing University of Chinese Medicine, Nanjing 210046, Jiangsu Province, China ; Nanjing BenQ Hospital, Nanjing 210036, Jiangsu Province, China.
authors.forename: Chunxiang
authors.initials: C
authors.lastname: Zhou
authors.affiliation: Basic Medicine College, Nanjing University of Chinese Medicine, Nanjing 210046, Jiangsu Province, China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Ling
keywords: Alzheimer’s disease; BV-2; Lingguizhugan decoction; SH-SY5Y; inflammatory reaction; phlegm-warming and fluid-dispersing