title: Microwell array-mediated delivery of lipoplexes containing nucleic acids for enhanced therapeutic efficacy.
abstract: Many delivery methods have been developed to improve the therapeutic efficacy and facilitate the clinical translation of nucleic acids-based therapeutics. We present a facile microwell array to mediate the delivery of nucleic acids carried by lipoplexes, which combines the advantages of lipoplexes as an efficient carrier system, the surface mediated delivery, and the control of surface topography. This method shows much higher transfection efficiency than conventional transfection method for oligodeoxynucleotides and microRNAs, and thus significantly reduces the effective therapeutic dosages. Microwell array is also a very flexible platform. Multifunctional lipoplexes containing both nucleic acid therapeutics and imaging reagents can be easily prepared in the microwell array and efficiently delivered to cells, demonstrating its potential applications in theranostic medicine.
authors.affiliation: Department of Biomedical Engineering, University at Buffalo, State University of New York, 332 Bonner Hall, Buffalo, NY, 14260, USA, ywu32@buffalo.edu.
authors.forename: Yun
authors.initials: Y
authors.lastname: Wu
authors.forename: Daniel
authors.initials: D
authors.lastname: Gallego-Perez
authors.forename: L James
authors.initials: LJ
authors.lastname: Lee
mesh_terms: D045744:Cell Line, Tumor; D002784:Cholesterol / Q000737:chemistry; D004129:Dimethylpolysiloxanes / Q000737:chemistry; D016503:Drug Delivery Systems / Q000295:instrumentation / Q000379:methods*; D004847:Epithelial Cells / Q000378:metabolism* / Q000473:pathology; D005229:Fatty Acids, Monounsaturated / Q000737:chemistry; D006801:Humans; D008081:Liposomes / Q000737:chemistry / Q000378:metabolism*; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D008856:Microscopy, Fluorescence; D009757:Nylons / Q000737:chemistry; D010714:Phosphatidylethanolamines / Q000737:chemistry; D054735:Phosphorothioate Oligonucleotides / Q000235:genetics / Q000378:metabolism*; D011092:Polyethylene Glycols / Q000737:chemistry; D000644:Quaternary Ammonium Compounds / Q000737:chemistry; D020545:Respiratory Mucosa / Q000378:metabolism / Q000473:pathology; D013499:Surface Properties; D014162:Transfection
chemical_list: D004129:Dimethylpolysiloxanes; D005229:Fatty Acids, Monounsaturated; D008081:Liposomes; C532045:MIRN29a microRNA, human; D035683:MicroRNAs; D009757:Nylons; D010714:Phosphatidylethanolamines; D054735:Phosphorothioate Oligonucleotides; D000644:Quaternary Ammonium Compounds; C085707:poly(dimethylsiloxane)-polyamide copolymer; C107570:polyethylene glycol-distearoylphosphatidylethanolamine; D011092:Polyethylene Glycols; D002784:Cholesterol; C070046:1,2-dioleoyloxy-3-(trimethylammonium)propane