title: Isolation and characterization of vB_ArS-ArV2 - first Arthrobacter sp. infecting bacteriophage with completely sequenced genome.
abstract: This is the first report on a complete genome sequence and biological characterization of the phage that infects Arthrobacter. A novel virus vB_ArS-ArV2 (ArV2) was isolated from soil using Arthrobacter sp. 68b strain for phage propagation. Based on transmission electron microscopy, ArV2 belongs to the family Siphoviridae and has an isometric head (∼63 nm in diameter) with a non-contractile flexible tail (∼194×10 nm) and six short tail fibers. ArV2 possesses a linear, double-stranded DNA genome (37,372 bp) with a G+C content of 62.73%. The genome contains 68 ORFs yet encodes no tRNA genes. A total of 28 ArV2 ORFs have no known functions and lack any reliable database matches. Proteomic analysis led to the experimental identification of 14 virion proteins, including 9 that were predicted by bioinformatics approaches. Comparative phylogenetic analysis, based on the amino acid sequence alignment of conserved proteins, set ArV2 apart from other siphoviruses. The data presented here will help to advance our understanding of Arthrobacter phage population and will extend our knowledge about the interaction between this particular host and its phages.
authors.affiliation: Department of Molecular Microbiology and Biotechnology, Institute of Biochemistry, Vilnius University, Vilnius, Lithuania.
authors.forename: Eugenijus
authors.initials: E
authors.lastname: Šimoliūnas
authors.affiliation: Department of Molecular Microbiology and Biotechnology, Institute of Biochemistry, Vilnius University, Vilnius, Lithuania.
authors.forename: Laura
authors.initials: L
authors.lastname: Kaliniene
authors.affiliation: Department of Molecular Microbiology and Biotechnology, Institute of Biochemistry, Vilnius University, Vilnius, Lithuania.
authors.forename: Miroslav
authors.initials: M
authors.lastname: Stasilo
authors.affiliation: Department of Molecular Microbiology and Biotechnology, Institute of Biochemistry, Vilnius University, Vilnius, Lithuania.
authors.forename: Lidija
authors.initials: L
authors.lastname: Truncaitė
authors.affiliation: Department of Molecular Microbiology and Biotechnology, Institute of Biochemistry, Vilnius University, Vilnius, Lithuania.
authors.forename: Aurelija
authors.initials: A
authors.lastname: Zajančkauskaitė
authors.affiliation: Laboratory of Plant Viruses, Institute of Botany, Nature Research Centre, Vilnius, Lithuania.
authors.forename: Juozas
authors.initials: J
authors.lastname: Staniulis
authors.affiliation: Department of Eukaryote Gene Engineering, Institute of Biotechnology, Vilnius University, Vilnius, Lithuania.
authors.forename: Juozas
authors.initials: J
authors.lastname: Nainys
authors.affiliation: Proteomics Centre, Institute of Biochemistry, Vilnius University, Vilnius, Lithuania.
authors.forename: Algirdas
authors.initials: A
authors.lastname: Kaupinis
authors.affiliation: Proteomics Centre, Institute of Biochemistry, Vilnius University, Vilnius, Lithuania.
authors.forename: Mindaugas
authors.initials: M
authors.lastname: Valius
authors.affiliation: Department of Molecular Microbiology and Biotechnology, Institute of Biochemistry, Vilnius University, Vilnius, Lithuania.
authors.forename: Rolandas
authors.initials: R
authors.lastname: Meškys
mesh_terms: D000595:Amino Acid Sequence / Q000235:genetics*; D001173:Arthrobacter / Q000235:genetics* / Q000821:virology; D001435:Bacteriophages / Q000235:genetics*; D017124:Conserved Sequence; D016679:Genome, Viral; D058977:Molecular Sequence Annotation; D008969:Molecular Sequence Data; D010802:Phylogeny; D017422:Sequence Analysis, DNA