title: The complete mitochondrial genome of the invasive Ponto-Caspian goby Ponticola kessleri obtained from high-throughput sequencing using the Ion Torrent Personal Genome Machine.
abstract: We report the first complete mitochondrial genome (mitogenome) of an invasive Ponto-Caspian goby, Ponticola kessleri (bighead goby, Günther 1891). Ion Torrent PGM sequencing of total DNA from two individuals yielded a contig of 16,971 bp, with overlapping ends located in the repetitive control region, which was validated using Sanger sequencing. The final mitogenome of Ponticola kessleri has a size of 16,890 bp and contains the expected gene configuration of 13 protein-coding genes, 2 rRNA genes and 22 tRNA genes. In a comparison with complete mitogenomes from other goby species, we identified a translocation of tRNA-Glu in the mitogenome of P. kessleri. Rearrangements are unique and rare events, and can thus provide phylogenetic information.
authors.affiliation: a Department of Environmental Science , Programm MGU, University of Basel , Basel , Switzerland and.
authors.forename: Irene
authors.initials: I
authors.lastname: Kalchhauser
authors.affiliation: b Biodiversität und Klima Forschungszentrum , Senckenberganlage 25 , Frankfurt am Main , Germany.
authors.forename: Verena E
authors.initials: VE
authors.lastname: Kutschera
authors.affiliation: a Department of Environmental Science , Programm MGU, University of Basel , Basel , Switzerland and.
authors.forename: Patricia
authors.initials: P
authors.lastname: Burkhardt-Holm
mesh_terms: D000818:Animals; D001482:Base Composition / Q000235:genetics; D020029:Base Pairing / Q000235:genetics; D004272:DNA, Mitochondrial / Q000235:genetics; D050259:Genes, Mitochondrial; D054629:Genome, Mitochondrial; D059014:High-Throughput Nucleotide Sequencing / Q000295:instrumentation* / Q000379:methods*; D010473:Perciformes / Q000235:genetics*; D012343:RNA, Transfer / Q000235:genetics
chemical_list: D004272:DNA, Mitochondrial; D012343:RNA, Transfer
keywords: Bighead goby; Ponticola kessleri; Rhine; high throughput sequencing; invasive; mitochondrial genome