title: Dynamic changes in dna methylation status in peripheral blood Mononuclear cells following an acute bout of exercise: Potential impact of exercise-induced elevations in interleukin-6 concentration.
abstract: The aim of the present study was to examine the relationship between interleukin (IL)-6 concentrations and DNA methylation in the peripheral blood mononuclear cells (PBMCs) of trained runners after a bout of prolonged, strenuous exercise. Eight healthy trained males completed a treadmill run at 60% vVO(2max) for 120 min followed by a 5-km time trial in a fasted condition. Whole blood samples were taken prior to, immediately before and 24 h following exercise. From these samples, PBMCs were isolated for analysis and plasma IL-6 concentrations were measured. The methylation status of DNA extracted from PBMCs was analysed using the Illumina 27k methylation beadchip platform. Global DNA methylation status was unaltered immediately and up to 24 hours following a bout of prolonged exercise in comparison to pre-exercise. Despite no change in global DNA methylation, plasma IL-6 concentrations were significantly related to the DNA methylation status of 11 genes. Our study demonstrates that the methylome is stable, while discovering a novel link between exercise-induced increases in circulating IL-6 and the DNA methylation status of 11 individual genes. Based on our preliminary findings, the mechanisms by which changes in plasma IL-6 concentrations and DNA methylation in response to exercise interact require further study.
authors.affiliation: Faculty of Health and Life Sciences, Northumbria University, Newcastle-upon-Tyne, UK.
authors.forename: P J
authors.initials: PJ
authors.lastname: Robson-Ansley
authors.affiliation: Department of Physiology and Pharmacology, Karolinska Institutet, Sweden.
authors.forename: A
authors.initials: A
authors.lastname: Saini
authors.affiliation: Faculty of Health and Life Sciences, Northumbria University, Newcastle-upon-Tyne, UK.
authors.forename: C
authors.initials: C
authors.lastname: Toms
authors.affiliation: Faculty of Health and Life Sciences, Northumbria University, Newcastle-upon-Tyne, UK.
authors.forename: L
authors.initials: L
authors.lastname: Ansley
authors.affiliation: School of Sport, University of Stirling, Stirling, UK.
authors.forename: I H
authors.initials: IH
authors.lastname: Walshe
authors.affiliation: School of Sport, Exercise and Health, Loughborough, UK.
authors.forename: M A
authors.initials: MA
authors.lastname: Nimmo
authors.affiliation: The University of Manchester, Manchester Academic Health Science Centre, University Hospital of South Manchester NHS Foundation Trust, Manchester, UK.
authors.forename: J A
authors.initials: JA
authors.lastname: Curtin
mesh_terms: D000328:Adult; D019175:DNA Methylation / Q000502:physiology*; D015444:Exercise / Q000502:physiology*; D006801:Humans; D015850:Interleukin-6 / Q000097:blood*; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D008297:Male; D012420:Running / Q000502:physiology*
chemical_list: C508600:IL6 protein, human; D015850:Interleukin-6