title: Development of an immunologically tolerated combination of fluorescent proteins for in vivo two-photon imaging.
abstract: Combinations of fluorescent proteins (FPs) are routinely used for multi-parameter in vivo imaging experiments to visualize tagged proteins or cell populations of interest. Studies involving FPs are often limited by spectral overlap, toxicity, relative quantum efficiency, and the potential for immunological rejection upon transfer into a non-tolerant recipient. Here we evaluate the immunologic visibility of several commonly used FPs by the murine immune system and identify a spectrally compatible, immunologically tolerated combination of FPs well suited for in vivo two-photon imaging.
authors.affiliation: National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892.
authors.forename: Selamawit
authors.initials: S
authors.lastname: Gossa
authors.affiliation: National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892.
authors.forename: Debasis
authors.initials: D
authors.lastname: Nayak
authors.affiliation: National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892.
authors.forename: Bernd H
authors.initials: BH
authors.lastname: Zinselmeyer
authors.affiliation: National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892.
authors.forename: Dorian B
authors.initials: DB
authors.lastname: McGavern
mesh_terms: D000818:Animals; D003952:Diagnostic Imaging; D031541:Fluorescence Resonance Energy Transfer; D049452:Green Fluorescent Proteins / Q000276:immunology / Q000302:isolation & purification*; D007107:Immune System / Q000648:ultrastructure*; D007108:Immune Tolerance; D051379:Mice; D017785:Photons
chemical_list: D049452:Green Fluorescent Proteins