title: One long oligonucleotide or two short oligonucleotides based shRNA construction and expression.
abstract: RNA interference (RNAi) has become a powerful and commonly used genetic tool for studying gene function. Vector-based short hairpin RNAs (shRNAs) trigger long-lasting RNAi effects compared to chemically synthesized siRNAs especially when they are imbedded in a lentiviral vector. In this chapter, a cost-effective method for shRNA design and expression is described. The strategy uses a carefully selected shRNA loop sequence and an antisense-loop-sense stem structure. Single and multiple shRNAs were expressed from the same vector, and thus the current design strategy has a great application potential in genomics.
authors.affiliation: Department of Biotechnology, Beijing Institute of Radiation Medicine, 27 Taiping Road, Beijing, 100850, People's Republic of China.
authors.forename: Xue-jun
authors.initials: XJ
authors.lastname: Wang
authors.forename: Sheng-qi
authors.initials: SQ
authors.lastname: Wang
mesh_terms: D001483:Base Sequence; D004262:DNA Restriction Enzymes / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D005822:Genetic Vectors; D055029:Inverted Repeat Sequences; D016086:Lentivirus / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D009841:Oligonucleotides / Q000737:chemistry* / Q000378:metabolism; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D013329:Structure-Activity Relationship
chemical_list: D009841:Oligonucleotides; D034741:RNA, Small Interfering; D004262:DNA Restriction Enzymes