title: Physiochemical and biological properties of phosphorylated polysaccharides from Dictyophora indusiata.
abstract: In this study, we aim to investigate the physiochemical and biological properties of water-soluble phosphorylated polysaccharides (P-DIP) obtained from a water-insoluble polysaccharide (DIP) extracted from Dictyophora indusiata. A series of physiochemical properties were determined, including morphology, water-solubility, molecular weight, and degree of substitution (DS). To investigate the antioxidant activity of P-DIP, we determined the scavenging activity of hydroxyl radicals and DPPH, as well as the reducing power. MTT assay was performed to determine the cytotoxic effects of DIP and P-DIP on the cellular proliferation of MCF-7 and B16 cells. Compared with DIP, P-DIP showed a satisfactory water-solubility and significant increase in the antioxidant properties. Moreover, P-DIP also showed more significant inhibitory effects on the growth of MCF-7 and B16 tumor cells than the water-insoluble DIP. These results indicated that phosphorylation might contribute to the improvement of water solubility, as well as antioxidant and anti-tumor activities of natural DIP.
authors.forename: Chao
authors.initials: C
authors.lastname: Deng
authors.forename: Haitian
authors.initials: H
authors.lastname: Fu
authors.forename: Jingjing
authors.initials: J
authors.lastname: Xu
authors.forename: Jingying
authors.initials: J
authors.lastname: Shang
authors.forename: Yongmei
authors.initials: Y
authors.lastname: Cheng
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology; D001487:Basidiomycota / Q000737:chemistry*; D045744:Cell Line, Tumor; D004305:Dose-Response Relationship, Drug; D062610:Fungal Polysaccharides / Q000737:chemistry* / Q000494:pharmacology*; D006801:Humans; D019906:Nuclear Magnetic Resonance, Biomolecular; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D000970:Antineoplastic Agents; D000975:Antioxidants; D062610:Fungal Polysaccharides