title: The Comparative Genomics and Phylogenomics of Leishmania amazonensis Parasite.
abstract: Leishmaniasis is an infectious disease caused by Leishmania species. Leishmania amazonensis is a New World Leishmania species belonging to the Mexicana complex, which is able to cause all types of leishmaniasis infections. The L. amazonensis reference strain MHOM/BR/1973/M2269 was sequenced identifying 8,802 codifying sequences (CDS), most of them of hypothetical function. Comparative analysis using six Leishmania species showed a core set of 7,016 orthologs. L. amazonensis and Leishmania mexicana share the largest number of distinct orthologs, while Leishmania braziliensis presented the largest number of inparalogs. Additionally, phylogenomic analysis confirmed the taxonomic position for L. amazonensis within the "Mexicana complex", reinforcing understanding of the split of New and Old World Leishmania. Potential non-homologous isofunctional enzymes (NISE) were identified between L. amazonensis and Homo sapiens that could provide new drug targets for development.
authors.affiliation: Pólo de Biologia Computacional e Sistemas, Instituto Oswaldo Cruz (Fiocruz/IOC), Rio de Janeiro, RJ, Brazil. ; Laboratório de Biologia Computacional e Sistemas, Instituto Oswaldo Cruz, Fiocruz, Rio de Janeiro, RJ, Brazil.
authors.forename: Diogo A
authors.initials: DA
authors.lastname: Tschoeke
authors.affiliation: Laboratório de Biologia Computacional e Sistemas, Instituto Oswaldo Cruz, Fiocruz, Rio de Janeiro, RJ, Brazil.
authors.forename: Gisele L
authors.initials: GL
authors.lastname: Nunes
authors.affiliation: Pólo de Biologia Computacional e Sistemas, Instituto Oswaldo Cruz (Fiocruz/IOC), Rio de Janeiro, RJ, Brazil. ; Laboratório de Biologia Computacional e Sistemas, Instituto Oswaldo Cruz, Fiocruz, Rio de Janeiro, RJ, Brazil.
authors.forename: Rodrigo
authors.initials: R
authors.lastname: Jardim
authors.affiliation: Laboratório de Biologia Computacional e Sistemas, Instituto Oswaldo Cruz, Fiocruz, Rio de Janeiro, RJ, Brazil.
authors.forename: Joana
authors.initials: J
authors.lastname: Lima
authors.affiliation: Laboratório de Biologia Computacional e Sistemas, Instituto Oswaldo Cruz, Fiocruz, Rio de Janeiro, RJ, Brazil.
authors.forename: Aline Sr
authors.initials: AS
authors.lastname: Dumaresq
authors.affiliation: Laboratório de Biologia Computacional e Sistemas, Instituto Oswaldo Cruz, Fiocruz, Rio de Janeiro, RJ, Brazil.
authors.forename: Monete R
authors.initials: MR
authors.lastname: Gomes
authors.affiliation: Laboratório de Biologia Computacional e Sistemas, Instituto Oswaldo Cruz, Fiocruz, Rio de Janeiro, RJ, Brazil.
authors.forename: Leandro
authors.initials: L
authors.lastname: de Mattos Pereira
authors.affiliation: Pólo de Biologia Computacional e Sistemas, Instituto Oswaldo Cruz (Fiocruz/IOC), Rio de Janeiro, RJ, Brazil.
authors.forename: Daniel R
authors.initials: DR
authors.lastname: Loureiro
authors.affiliation: Laboratório de Protozoologia, Universidade Federal de Santa Catarina, Florianópolis, SC, Brazil.
authors.forename: Patricia H
authors.initials: PH
authors.lastname: Stoco
authors.affiliation: Laboratório de Inflamação Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil. ; Wellcome Trust Centre for Molecular Parasitology, Institute of Immunity, Infection and Inflammation, College of MVLS, University of Glasgow, Glasgow, UK.
authors.forename: Herbert Leonel
authors.initials: HL
authors.lastname: de Matos Guedes
authors.affiliation: Pólo de Biologia Computacional e Sistemas, Instituto Oswaldo Cruz (Fiocruz/IOC), Rio de Janeiro, RJ, Brazil. ; Laboratório de Biologia Computacional e Sistemas, Instituto Oswaldo Cruz, Fiocruz, Rio de Janeiro, RJ, Brazil.
authors.forename: Antonio Basilio
authors.initials: AB
authors.lastname: de Miranda
authors.affiliation: Pólo de Biologia Computacional e Sistemas, Instituto Oswaldo Cruz (Fiocruz/IOC), Rio de Janeiro, RJ, Brazil. ; Instituto René Rachou (Fiocruz/IRR), Belo Horizonte, MG, Brazil.
authors.forename: Jeronimo
authors.initials: J
authors.lastname: Ruiz
authors.affiliation: Laboratório de Biologia Molecular de Parasitas e Vetores, Instituto Oswaldo Cruz, Fiocruz, Rio de Janeiro, RJ, Brazil.
authors.forename: André
authors.initials: A
authors.lastname: Pitaluga
authors.affiliation: Pólo de Biologia Computacional e Sistemas, Instituto Oswaldo Cruz (Fiocruz/IOC), Rio de Janeiro, RJ, Brazil. ; Laboratório de Bioquímica de Proteínas e Peptídeos, Instituto Oswaldo Cruz, Fiocruz, Rio de Janeiro, RJ, Brazil.
authors.forename: Floriano P
authors.initials: FP
authors.lastname: Silva
authors.affiliation: Pólo de Biologia Computacional e Sistemas, Instituto Oswaldo Cruz (Fiocruz/IOC), Rio de Janeiro, RJ, Brazil. ; Instituto Carlos Chagas (Fiocruz/ICC), Curitiba, PR, Brazil.
authors.forename: Christian M
authors.initials: CM
authors.lastname: Probst
authors.affiliation: Wellcome Trust Centre for Molecular Parasitology, Institute of Immunity, Infection and Inflammation, College of MVLS, University of Glasgow, Glasgow, UK.
authors.forename: Nicholas J
authors.initials: NJ
authors.lastname: Dickens
authors.affiliation: Wellcome Trust Centre for Molecular Parasitology, Institute of Immunity, Infection and Inflammation, College of MVLS, University of Glasgow, Glasgow, UK.
authors.forename: Jeremy C
authors.initials: JC
authors.lastname: Mottram
authors.affiliation: Laboratório de Protozoologia, Universidade Federal de Santa Catarina, Florianópolis, SC, Brazil.
authors.forename: Edmundo C
authors.initials: EC
authors.lastname: Grisard
authors.affiliation: Pólo de Biologia Computacional e Sistemas, Instituto Oswaldo Cruz (Fiocruz/IOC), Rio de Janeiro, RJ, Brazil. ; Laboratório de Biologia Computacional e Sistemas, Instituto Oswaldo Cruz, Fiocruz, Rio de Janeiro, RJ, Brazil.
authors.forename: Alberto Mr
authors.initials: AM
authors.lastname: Dávila
keywords: Leishmania amazonensis; comparative genomics; phylogenomics