title: Expression pattern of the PRDX2, RAB1A, RAB1B, RAB5A and RAB25 genes in normal and cancer cervical tissues.
abstract: Cervical cancer is the second most prevalent malignancy among women worldwide, and additional objective diagnostic markers for this disease are needed. Given the link between cancer development and alternative splicing, we aimed to analyze the splicing patterns of the PRDX2, RAB1A, RAB1B, RAB5A and RAB25 genes, which are associated with different cancers, in normal cervical tissue, preinvasive cervical lesions and invasive cervical tumors, to identify new objective diagnostic markers. Biopsies of normal cervical tissue, preinvasive cervical lesions and invasive cervical tumors, were subjected to rapid amplification of cDNA 3' ends (3' RACE) RT‑PCR. Resulting PCR products were analyzed on agarose gels, gel‑purified and sequenced. Normal cervical tissue, preinvasive cervical lesions and invasive cervical tumors contained one PCR product corresponding to full‑length PRDX2, RAB5A and RAB25 transcripts. All tissues contained two RAB1A‑specific PCR products corresponding to the full‑length transcript and one new alternatively spliced RAB1A transcript. Invasive cervical tumors contained one PCR product corresponding to the full‑length RAB1B transcript, while all normal cervical tissue and preinvasive cervical lesions contained both the full‑length RAB1B transcript and three new alternatively spliced RAB1B transcripts. Alternative splicing of the RAB1A transcript occurs in all cervical tissues, while alternative splicing of the RAB1B transcript occurs in normal cervical tissue and in preinvasive cervical lesions; not in invasive cervical tumors.
authors.affiliation: Department of Women's and Children's Health, Division of Obstetrics and Gynecology, Karolinska Institute, Karolinska University Hospital Solna, 171 76 Stockholm, Sweden.
authors.forename: Andrej
authors.initials: A
authors.lastname: Nikoshkov
authors.affiliation: Department of Medicine Solna, Unit of Infectious Diseases, Center for Molecular Medicine, Karolinska Institute, Karolinska University Hospital, 171 76 Stockholm, Sweden.
authors.forename: Kristina
authors.initials: K
authors.lastname: Broliden
authors.affiliation: Department of Women's and Children's Health, Division of Obstetrics and Gynecology, Karolinska Institute, Karolinska University Hospital Solna, 171 76 Stockholm, Sweden.
authors.forename: Sanaz
authors.initials: S
authors.lastname: Attarha
authors.affiliation: Department of Oncology‑Pathology, Karolinska Institute, 171 76 Stockholm, Sweden.
authors.forename: Vitali
authors.initials: V
authors.lastname: Sviatoha
authors.affiliation: Department of Gynecological Oncology, Radiumhemmet, Karolinska University Hospital, 171 76 Stockholm, Sweden.
authors.forename: Ann-Cathrin
authors.initials: AC
authors.lastname: Hellström
authors.affiliation: Department of Women's and Children's Health, Division of Obstetrics and Gynecology, Karolinska Institute, Karolinska University Hospital Solna, 171 76 Stockholm, Sweden.
authors.forename: Miriam
authors.initials: M
authors.lastname: Mints
authors.affiliation: Department of Women's and Children's Health, Division of Obstetrics and Gynecology, Karolinska Institute, Karolinska University Hospital Solna, 171 76 Stockholm, Sweden.
authors.forename: Sonia
authors.initials: S
authors.lastname: Andersson
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002584:Cervix Uteri / Q000378:metabolism / Q000473:pathology; D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008969:Molecular Sequence Data; D009361:Neoplasm Invasiveness; D054464:Peroxiredoxins / Q000235:genetics* / Q000378:metabolism; D011230:Precancerous Conditions / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D002583:Uterine Cervical Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D020691:rab GTP-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D020761:rab1 GTP-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D020696:rab5 GTP-Binding Proteins / Q000235:genetics* / Q000378:metabolism
chemical_list: C509763:Rab25 protein, human; C086065:PRDX2 protein, human; D054464:Peroxiredoxins; C427755:RAB5C protein, human; C529587:Rab1B protein, human; D020691:rab GTP-Binding Proteins; D020761:rab1 GTP-Binding Proteins; D020696:rab5 GTP-Binding Proteins