title: Mesoporous silica particles potentiate antigen-specific T-cell responses.
abstract: OBJECTIVE
  To study the adjuvant effect of mesoporous silica particles and their capability of modifying an already existing allergic Th2-like immune response.
  
  
  METHODS
  The adjuvant effect of Santa Barbara Amorphous-15 (SBA-15) mesoporous silica particles was studied in an antigen-specific ovalbumin (OVA) system in vitro and in vivo. The capacity of the OVA-loaded SBA-15 particles (SBA-15-OVA) to modify an existing immune response was assessed in a murine allergy model.
  
  
  RESULTS
  SBA-15-OVA induced significantly stronger OVA-specific splenocyte proliferation compared with OVA alone. Significantly higher IFN-γ production was observed in ex vivo OVA-stimulated splenocytes from SBA-15-OVA-immunized mice compared with mice injected with only SBA-15 or OVA. Treatment of OVA-sensitized mice with SBA-15-OVA modified the immune response with significantly lower serum levels of OVA-specific IgE and higher IgG levels compared with the alum-OVA-treated group.
  
  
  CONCLUSIONS
  The results are promising for the continued development of mesoporous silica materials for therapeutic applications.
authors.affiliation: Nanotechnology & Functional Materials, Department of Engineering Sciences, the Ångström Laboratory, Uppsala University, 751 21 Uppsala, Sweden.
authors.forename: Natalia
authors.initials: N
authors.lastname: Kupferschmidt
authors.forename: Khaleda Rahman
authors.initials: KR
authors.lastname: Qazi
authors.forename: Cecilia
authors.initials: C
authors.lastname: Kemi
authors.forename: Helen
authors.initials: H
authors.lastname: Vallhov
authors.forename: Alfonso E
authors.initials: AE
authors.lastname: Garcia-Bennett
authors.forename: Susanne
authors.initials: S
authors.lastname: Gabrielsson
authors.forename: Annika
authors.initials: A
authors.lastname: Scheynius
mesh_terms: D000276:Adjuvants, Immunologic / Q000008:administration & dosage*; D000818:Animals; D000918:Antibody Specificity; D000941:Antigens / Q000008:administration & dosage* / Q000378:metabolism; D001205:Ascorbic Acid / Q000031:analogs & derivatives; D049109:Cell Proliferation; D005260:Female; D007371:Interferon-gamma / Q000096:biosynthesis; D051379:Mice; D008807:Mice, Inbred BALB C; D008822:Mice, Transgenic; D008855:Microscopy, Electron, Scanning; D018448:Models, Immunological; D050997:Nanomedicine; D053758:Nanoparticles / Q000008:administration & dosage / Q000737:chemistry / Q000648:ultrastructure; D010047:Ovalbumin / Q000008:administration & dosage / Q000493:pharmacokinetics; D010316:Particle Size; D012822:Silicon Dioxide / Q000008:administration & dosage*; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology*
chemical_list: D000276:Adjuvants, Immunologic; D000941:Antigens; C509969:SBA-15; D012822:Silicon Dioxide; C087337:6-S-hexadecyl-2-methoxythioascorbic acid; D007371:Interferon-gamma; D010047:Ovalbumin; D001205:Ascorbic Acid
keywords: T-cell; adjuvant; allergy model; cytokine; mesoporous silica particle; ovalbumin; splenocyte