title: Biotransformation of catechin and extraction of active polysaccharide from green tea leaves via simultaneous treatment with tannase and pectinase.
abstract: BACKGROUND
  Green tea is a dietary source of bioactive compounds for human health. Enzymatic treatments induce the bioconversion of bioactive components, which can improve biological activities. In this study, we investigated the effect of simultaneous treatment with tannase and Rapidase on biotransformation of catechins and extraction of polysaccharide from green tea extract (GTE).
  
  
  RESULTS
  Tannase and pectinase treatments induced the biotransformation of catechins and altered tea polysaccharide () content. The addition of GTE to the enzyme reaction resulted in a significant increase in degallated catechins, including gallic acid, a product of the tannase reaction (314.5-4076.0 µg mL(-1)) and a reduction in epigallocatechin gallate (EGCG). Biotransformation of catechins improved the radical scavenging activity of GTE. Pectinase treatment led to change of TPS composition in GTE by hydrolyzing polysaccharides. In addition, pectinase-driven hydrolysis in polysaccharides significantly increased TPS-induced Interleukin 6 (IL-6) production in macrophages. In particular, treatment of Rapidase (TPS-Ra) led to the highest IL-6 production among TPS samples, similar to treatment of highly purified pectinase (TPS-GTE), a positive control.
  
  
  CONCLUSIONS
  Simultaneous processing with tannase and Rapidase can be an efficient method for the extraction of bioactive polysaccharides and biotransformation of catechins with enhanced radical scavenging activity from green tea.
authors.affiliation: Cosmax Bio INC, Jecheon, 390-250, Republic of Korea.
authors.forename: Joo Hyun
authors.initials: JH
authors.lastname: Baik
authors.affiliation: Department of Food Science and Biotechnology, Kyonggi University, Suwon, 443-760, Republic of Korea.
authors.forename: Kwang-Soon
authors.initials: KS
authors.lastname: Shin
authors.affiliation: Department of Food and Nutrition, Korea University, Seoul, 136-703, Republic of Korea.
authors.forename: Yooheon
authors.initials: Y
authors.lastname: Park
authors.affiliation: Department of Food and Nutrition, Korea National University of Transportation, Jeungpyeong, 368-701, Republic of Korea.
authors.forename: Kwang-Won
authors.initials: KW
authors.lastname: Yu
authors.affiliation: Department of Food and Nutrition, Korea University, Seoul, 136-703, Republic of Korea.
authors.forename: Hyung Joo
authors.initials: HJ
authors.lastname: Suh
authors.affiliation: Department of Food Science and Technology, Seoul Women's University, Seoul, 139-774, Republic of Korea.
authors.forename: Hyeon-Son
authors.initials: HS
authors.lastname: Choi
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D001711:Biotransformation; D028241:Camellia sinensis / Q000737:chemistry*; D002265:Carboxylic Ester Hydrolases / Q000378:metabolism*; D002392:Catechin / Q000031:analogs & derivatives / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D005707:Gallic Acid / Q000378:metabolism; D006801:Humans; D006868:Hydrolysis; D015850:Interleukin-6 / Q000378:metabolism; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism; D008807:Mice, Inbred BALB C; D010936:Plant Extracts / Q000737:chemistry* / Q000494:pharmacology; D018515:Plant Leaves / Q000737:chemistry; D011096:Polygalacturonase / Q000378:metabolism*; D011134:Polysaccharides / Q000302:isolation & purification* / Q000494:pharmacology; D013662:Tea / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D015850:Interleukin-6; D010936:Plant Extracts; D011134:Polysaccharides; D013662:Tea; D005707:Gallic Acid; D002392:Catechin; C045651:epigallocatechin gallate; D002265:Carboxylic Ester Hydrolases; C022711:tannase; D011096:Polygalacturonase
keywords: IL-6 production; biotransformation; radical scavenging activity; simultaneous enzymatic process; tea polysaccharide