title: MicroRNA expression profiling altered by variant dosage of radiation exposure.
abstract: Various biological effects are associated with radiation exposure. Irradiated cells may elevate the risk for genetic instability, mutation, and cancer under low levels of radiation exposure, in addition to being able to extend the postradiation side effects in normal tissues. Radiation-induced bystander effect (RIBE) is the focus of rigorous research as it may promote the development of cancer even at low radiation doses. Alterations in the DNA sequence could not explain these biological effects of radiation and it is thought that epigenetics factors may be involved. Indeed, some microRNAs (or miRNAs) have been found to correlate radiation-induced damages and may be potential biomarkers for the various biological effects caused by different levels of radiation exposure. However, the regulatory role that miRNA plays in this aspect remains elusive. In this study, we profiled the expression changes in miRNA under fractionated radiation exposure in human peripheral blood mononuclear cells. By utilizing publicly available microRNA knowledge bases and performing cross validations with our previous gene expression profiling under the same radiation condition, we identified various miRNA-gene interactions specific to different doses of radiation treatment, providing new insights for the molecular underpinnings of radiation injury.
authors.affiliation: Institute of Medical Sciences, Tzu Chi University, No. 701, Zhongyang Road, Section 3, Hualien 97004, Taiwan ; Laboratory for Cytogenetics, Center for Genetic Counseling, Buddhist Tzu Chi General Hospital, Hualien 97004, Taiwan.
authors.forename: Kuei-Fang
authors.initials: KF
authors.lastname: Lee
authors.affiliation: Department of Computer Science & Information Engineering, Tamkang University, New Taipei City 25137, Taiwan.
authors.forename: Yi-Cheng
authors.identifier: 0000-0001-7153-0410
authors.initials: YC
authors.lastname: Chen
authors.affiliation: Bioinformatics Core Laboratory, Institute of Molecular Biology, Academia Sinica, Taipei 11529, Taiwan.
authors.forename: Paul Wei-Che
authors.initials: PW
authors.lastname: Hsu
authors.affiliation: Department of Molecular Biology and Human Genetics, Tzu Chi University, Hualien 97004, Taiwan.
authors.forename: Ingrid Y
authors.initials: IY
authors.lastname: Liu
authors.affiliation: Institute of Medical Sciences, Tzu Chi University, No. 701, Zhongyang Road, Section 3, Hualien 97004, Taiwan.
authors.forename: Lawrence Shih-Hsin
authors.initials: LS
authors.lastname: Wu
mesh_terms: D002453:Cell Cycle / Q000235:genetics / Q000528:radiation effects; D045744:Cell Line, Tumor; D003037:Cobalt Radioisotopes; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic / Q000528:radiation effects; D053263:Gene Regulatory Networks / Q000235:genetics / Q000528:radiation effects; D052138:Genes, Neoplasm; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000378:metabolism / Q000528:radiation effects; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D011829:Radiation Dosage; D015203:Reproducibility of Results
chemical_list: D003037:Cobalt Radioisotopes; D035683:MicroRNAs