title: Variation of high-molecular-weight secalin subunit composition in Rye (Secale cereale L.) inbred lines.
abstract: In this study, identification and characterization of the rye HMW secalin subunit (HMW-SS) composition in 68 inbred rye (Secale cereale L.) lines was performed by capillary zone electrophoresis (CZE). The HMW-SS were separated in an uncoated fused-silica capillary using an isoelectric iminodiacetic buffer in combination with poly(ethylene oxide), lauryl sulfobetaine, and acetonitrile as the separation buffer. The separations of the nonalkylated HMW-SS provided very good resolution and high reproducibility. Generally, the x-type rye HMW-SS were more abundant and have longer migration times than the y-type subunits. Both types of rye HMW-SS were separated into the major protein peak and one or two minor peaks. In total, seven x-type HMW-SS, five of which were newly identified subunits, and six y-type subunits, four of which were new, were distinguished on the basis of their CZE migration times. The migration order of the rye HMW-SS using CZE differed considerably from the relative electrophoretic mobilities in the SDS-PAGE gels.
authors.affiliation: Institute of Plant Genetics, Polish Academy of Sciences , PL 60-479 Poznań, Poland.
authors.forename: Bolesław P
authors.initials: BP
authors.lastname: Salmanowicz
authors.forename: Monika
authors.initials: M
authors.lastname: Langner
authors.forename: Helena
authors.initials: H
authors.lastname: Kubicka-Matusiewicz
mesh_terms: D019075:Electrophoresis, Capillary; D004591:Electrophoresis, Polyacrylamide Gel; D005983:Glutens / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D007178:Inbreeding; D008970:Molecular Weight; D021122:Protein Subunits / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D012434:Secale / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
chemical_list: D021122:Protein Subunits; D005983:Glutens
keywords: Glu-R1 locus; HMW secalins; allelic variation; capillary zone electrophoresis; rye