title: Proteomic analysis of vestibular schwannoma: conflicting role of apoptosis on the pathophysiology of sporadic vestibular schwannoma.
abstract: OBJECTIVE
  In this study, we investigated the pathophysiology and mechanism underlying sporadic forms of vestibular schwannoma (VS) by comparing VS tissue with normal nerve tissue using proteomics.
  
  
  BACKGROUND
  Proteomic analysis by two-dimensional electrophoresis and matrix-assisted laser desorption and ionization time-of-flight mass spectrometry facilitates identification and characterization of specific proteins related to the pathogenesis of various diseases.
  
  
  METHODS
  Proteins were extracted from two vestibular nerve specimens and two VS specimens and analyzed in parallel using two-dimensional electrophoresis. We then analyzed 29 spots that were differentially expressed using matrix-assisted laser desorption and ionization time-of-flight mass spectrometry. Upregulated proteins associated with apoptosis were confirmed by Western blot analysis and immunohistochemistry.
  
  
  RESULTS
  Twenty-nine proteins showing significant changes in the expression level between VS tissue and normal nerve tissue were identified. Of these, seven proteins were related to apoptosis.
  
  
  CONCLUSIONS
  Our findings indicate that apoptosis is associated in a complex manner with the pathophysiology of VS. The suppression of apoptosis is presumably involved in tumor occurrence and, conversely, increased apoptotic expression may contribute to the slow tumor growth rate and may be correlated with the Antoni type B area.
authors.affiliation: *Department of Otolaryngology-Head and Neck Surgery, College of Medicine, The Catholic University of Korea, Seoul; and †Clinical Medicine Research Institute of Bucheon St. Mary's Hospital, The Catholic University of Korea, Bucheon, Republic of Korea.
authors.forename: Jae-Hyun
authors.initials: JH
authors.lastname: Seo
authors.forename: Kyoung-Ho
authors.initials: KH
authors.lastname: Park
authors.forename: Eun-Ju
authors.initials: EJ
authors.lastname: Jeon
authors.forename: Ki-Hong
authors.initials: KH
authors.lastname: Chang
authors.forename: Heejin
authors.initials: H
authors.lastname: Lee
authors.forename: Weonsun
authors.initials: W
authors.lastname: Lee
authors.forename: Yong-Soo
authors.initials: YS
authors.lastname: Park
mesh_terms: D017209:Apoptosis / Q000502:physiology*; D015153:Blotting, Western; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D007150:Immunohistochemistry; D009464:Neuroma, Acoustic / Q000378:metabolism*; D040901:Proteomics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D015854:Up-Regulation; D014725:Vestibular Nerve / Q000378:metabolism