title: Inhibitory effect of hexane fraction from Myagropsis myagroides on pancreatic α-amylase in vitro.
abstract: A Myagropsis myagroides (Mm) methanol extract showed α-amylase inhibitory activity of 13% at a concentration of 5 mg/ml. Results showed that the hexane fraction from the Mm methanol extract exhibited α-amylase inhibitory activity with an IC50 value of 4.24 mg/ml. The hexane fraction was separated using silica-gel column chromatography, and six subfractions were obtained. The fraction eluted with CHCl3:MeOH = 50:1 showed the highest α-amylase inhibitory activity with an IC50 value of 0.72 mg/ml. This fraction was purified using Sephadex LH-20 column chromatography and an octadecyl silica (ODS) Sepak cartridge, obtaining seven subfractions. Fraction (Fr.) 4 also showed a strong α-amylase inhibitory activity with an IC50 value of 0.75 mg/ml. Fr. 4 was purified by Sephadex LH-20 column chromatography and ODS Sepak cartridge, obtaining six subfractions. Fr. 4-2 was identified as sargachromanol I with an IC50 value of 0.40 mg/ml, and the inhibition pattern analyzed from Lineweaver-Burk plots revealed it to be an uncompetitive inhibitor. These results suggest that Mm has potential as a natural antidiabetes agent.
authors.affiliation: Department of Food Science and Technology/Institute of Food Science, Pukyong National University, Busan 608-737, Republic of Korea.
authors.forename: Won-Min
authors.initials: WM
authors.lastname: Pak
authors.forename: Koth-Bong-Woo Ri
authors.initials: KB
authors.lastname: Kim
authors.forename: Min-Ji
authors.initials: MJ
authors.lastname: Kim
authors.forename: Ji-Young
authors.initials: JY
authors.lastname: Cho
authors.forename: Dong-Hyun
authors.initials: DH
authors.lastname: Ahn
mesh_terms: D001578:Benzopyrans / Q000302:isolation & purification* / Q000494:pharmacology*; D002845:Chromatography / Q000379:methods; D003911:Dextrans; D004791:Enzyme Inhibitors / Q000302:isolation & purification* / Q000494:pharmacology*; D005233:Fatty Alcohols / Q000302:isolation & purification* / Q000494:pharmacology*; D006586:Hexanes; D000432:Methanol; D055292:Pancreatic alpha-Amylases / Q000037:antagonists & inhibitors* / Q000378:metabolism*; D000459:Phaeophyceae / Q000737:chemistry*
chemical_list: D001578:Benzopyrans; D003911:Dextrans; D004791:Enzyme Inhibitors; D005233:Fatty Alcohols; D006586:Hexanes; C000604109:sargachromanol I; C026385:n-hexane; C025614:sephadex; D055292:Pancreatic alpha-Amylases; D000432:Methanol