title: Immunochemical authentication of manuka honey using a monoclonal antibody specific to a glycoside of methyl syringate.
abstract: Leptosperin, a novel glycoside of methyl syringate, is exclusively present in manuka honey derived from the Leptospermum species Leptospermum scoparium. Quantification of leptosperin might thus be applicable for authentication of honey. The concentration of leptosperin has high linearity with antibacterial activity. We established a monoclonal antibody to leptosperin and characterized the antibody in detail by a competitive enzyme-linked immunosorbent assay (ELISA), comparing the results with those of the high-performance liquid chromatography (HPLC) method for validation. The antigen in manuka honey was confirmed as leptosperin by HPLC fractionation with quantitation by an ELISA. Leptosperin contents of 50 honey samples were analyzed by an established ELISA, which can handle 20 samples (duplicate) on one 96-well plate. Significant coincidence with the chemical quantitation was observed. Immunochemical quantitation of leptosperin would be an economical and facile method for the possible authentication of manuka honey, allowing many honey samples to be processed and analyzed by an ELISA simultaneously.
authors.affiliation: School of Human Science and Environment and ‡Research Institute for Food and Nutritional Sciences, University of Hyogo , Himeji, Hyogo 670-0092, Japan.
authors.forename: Yoji
authors.initials: Y
authors.lastname: Kato
authors.forename: Yukako
authors.initials: Y
authors.lastname: Araki
authors.forename: Maki
authors.initials: M
authors.lastname: Juri
authors.forename: Rie
authors.initials: R
authors.lastname: Fujinaka
authors.forename: Akari
authors.initials: A
authors.lastname: Ishisaka
authors.forename: Noritoshi
authors.initials: N
authors.lastname: Kitamoto
authors.forename: Yoko
authors.initials: Y
authors.lastname: Nitta
authors.forename: Toshio
authors.initials: T
authors.lastname: Niwa
authors.forename: Yosuke
authors.initials: Y
authors.lastname: Takimoto
mesh_terms: D000911:Antibodies, Monoclonal / Q000032:analysis; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005707:Gallic Acid / Q000031:analogs & derivatives* / Q000032:analysis; D006027:Glycosides / Q000032:analysis*; D006722:Honey / Q000032:analysis*; D031649:Leptospermum / Q000737:chemistry*
chemical_list: D000911:Antibodies, Monoclonal; D006027:Glycosides; C506133:methyl syringate; D005707:Gallic Acid
keywords: authentication; chemical marker; leptosperin; manuka honey; monoclonal antibody