title: Surface immuno-functionalisation for the capture and detection of Vibrio species in the marine environment: a new management tool for industrial facilities.
abstract: Bacteria from the genus Vibrio are a common and environmentally important group of bacteria within coastal environments and include species pathogenic to aquaculture organisms. Their distribution and abundance are linked to specific environmental parameters, including temperature, salinity and nutrient enrichment. Accurate and efficient detection of Vibrios in environmental samples provides a potential important indicator of overall ecosystem health while also allowing rapid management responses for species pathogenic to humans or species implicated in disease of economically important aquacultured fish and invertebrates. In this study, we developed a surface immuno-functionalisation protocol, based on an avidin-biotin type covalent binding strategy, allowing specific sandwich-type detection of bacteria from the Vibrio genus. The assay was optimized on 12 diverse Vibrio strains, including species that have implications for aquaculture industries, reaching detection limits between 7×10(3) to 3×10(4) cells mL(-1). Current techniques for the detection of total Vibrios rely on laborious or inefficient analyses resulting in delayed management decisions. This work represents a novel approach for a rapid, accurate, sensitive and robust tool for quantifying Vibrios directly in industrial systems and in the environment, thereby facilitating rapid management responses.
authors.affiliation: Plant Functional Biology and Climate Change Cluster (C3), University of Technology, Sydney, NSW, Australia.
authors.forename: Olivier F
authors.initials: OF
authors.lastname: Laczka
authors.affiliation: The ithree institute, University of Technology, Sydney, NSW, Australia.
authors.forename: Maurizio
authors.initials: M
authors.lastname: Labbate
authors.affiliation: Plant Functional Biology and Climate Change Cluster (C3), University of Technology, Sydney, NSW, Australia.
authors.forename: Justin R
authors.initials: JR
authors.lastname: Seymour
authors.affiliation: Australian Institute for Marine Science, Townsville, QLD, Australia.
authors.forename: David G
authors.initials: DG
authors.lastname: Bourne
authors.affiliation: Port Stephens Fisheries Institute, New South Wales Department of Primary Industries, Taylors Beach, NSW, Australia.
authors.forename: Stewart S
authors.initials: SS
authors.lastname: Fielder
authors.affiliation: Plant Functional Biology and Climate Change Cluster (C3), University of Technology, Sydney, NSW, Australia.
authors.forename: Martina A
authors.initials: MA
authors.lastname: Doblin
mesh_terms: D000818:Animals; D017756:Aquaculture / Q000379:methods*; D004777:Environment; D006801:Humans; D014733:Vibrio / Q000302:isolation & purification*; D014871:Water Microbiology