title: Function of interleukin-17 and -35 in the blood of patients with hepatitis B-related liver cirrhosis.
abstract: Intrahepatic T helper (Th)17 cytokine and serum interleukin (IL)-17 levels in patients with hepatitis B are positively correlated with the progression of liver cirrhosis (LC). IL-35 can significantly inhibit the differentiation of Th17 cells and the synthesis of IL-17. The present study aimed to investigate the function and expression of IL-17 and IL-35 in the blood of patients with hepatitis B‑related LC. The levels of IL-17 and IL-35 in the peripheral blood of 30 patients with chronic hepatitis B (CHB), 79 with LC, 14 with chronic severe hepatitis B (CSHB), and 20 normal controls were detected by ELISA. Quantitative polymerase chain reaction was used to evaluate Epstein-Barr virus-induced gene 3 (EBI3), forkhead box (FOX)P3 and IL-17 mRNA expression levels in peripheral blood mononuclear cells (PBMCs). Western blotting was used to determine protein expression. The liver function of patients and normal controls was measured. EBI3, IL-17 and FOXP3 mRNA expression levels in PBMCs from patients with LC, CHB and CSHB were higher than those in cells from the controls. IL-17 mRNA levels differed significantly according to the Child-Pugh classification and exhibited an upward trend over time in contrast to a downward trend for EBI3 and FOXP3 mRNA. The changes in protein expression in the peripheral blood were consistent with the changes in mRNA expression. Serum IL-17 levels were positively correlated with total bilirubin (TBIL), alanine aminotransferase (ALT) and Child-Pugh grade, and were negatively correlated with albumin. These observed differences were significant. Serum IL-35 levels were negatively correlated with albumin, but not with Child-Pugh grade, ALT and TBIL. IL-17 and IL-35 may be critically involved in the pathogenesis of hepatitis B-related LC.
authors.affiliation: Department of Gastroenterology, Shanghai Changning Central Hospital, Shanghai 200336, P.R. China.
authors.forename: Min
authors.initials: M
authors.lastname: Shi
authors.affiliation: Department of Gastroenterology, Shanghai Changning Central Hospital, Shanghai 200336, P.R. China.
authors.forename: Jue
authors.initials: J
authors.lastname: Wei
authors.affiliation: Department of Gastroenterology, Shanghai Changning Central Hospital, Shanghai 200336, P.R. China.
authors.forename: Jinbin
authors.initials: J
authors.lastname: Dong
authors.affiliation: Department of Gastroenterology, Shanghai Changning Central Hospital, Shanghai 200336, P.R. China.
authors.forename: Wenying
authors.initials: W
authors.lastname: Meng
authors.affiliation: Department of Gastroenterology, Shanghai Changning Central Hospital, Shanghai 200336, P.R. China.
authors.forename: Jiali
authors.initials: J
authors.lastname: Ma
authors.affiliation: Department of Gastroenterology, Shanghai Changning Central Hospital, Shanghai 200336, P.R. China.
authors.forename: Ting
authors.initials: T
authors.lastname: Wang
authors.affiliation: Department of Gastroenterology, Shanghai Changning Central Hospital, Shanghai 200336, P.R. China.
authors.forename: Na
authors.initials: N
authors.lastname: Wang
authors.affiliation: Department of Gastroenterology, Shanghai Changning Central Hospital, Shanghai 200336, P.R. China.
authors.forename: Yugang
authors.initials: Y
authors.lastname: Wang
mesh_terms: D000328:Adult; D005260:Female; D051858:Forkhead Transcription Factors / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D020869:Gene Expression Profiling; D006509:Hepatitis B; D019694:Hepatitis B, Chronic / Q000150:complications*; D006801:Humans; D020381:Interleukin-17 / Q000097:blood* / Q000235:genetics / Q000378:metabolism; D007378:Interleukins / Q000097:blood / Q000235:genetics / Q000378:metabolism; D008103:Liver Cirrhosis / Q000097:blood* / Q000209:etiology* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D015778:Minor Histocompatibility Antigens; D011485:Protein Binding
chemical_list: C097732:EBI3 protein, human; C418974:FOXP3 protein, human; D051858:Forkhead Transcription Factors; D020381:Interleukin-17; D007378:Interleukins; D015778:Minor Histocompatibility Antigens; C532417:interleukin-35, human