title: Oral delivery of highly lipophilic poorly water-soluble drugs: spray-dried dispersions to improve oral absorption and enable high-dose toxicology studies of a P2Y1 antagonist.
abstract: BMS-B is a highly lipophilic compound (clog P 7.72) with poor aqueous solubility (<10 ng/mL at pH 1 and 6.5). The compound exhibits low bioavailability in preclinical species when dosed as cosolvent solution formulations, with reduced exposure upon dose escalation. The purpose of this study was to evaluate spray-dried dispersions (SDDs) for enhancing oral exposure and enabling toxicology studies of BMS-B. SDD solids of BMS-B were prepared with 10%-25% drug in hydroxypropyl methylcellulose acetate succinate and showed an enhanced dissolution profile relative to the neat form of the compound. When dosed in rats and monkeys at 5 mg/kg, the SDD exhibited comparable exposure relative to the solution formulation. The SDD was also dosed in rats at 200 and 400 mg/kg and showed dose-proportional exposure compared to the solution formulation. Based on in vitro and in vivo data, the SDD formulation was selected for the toxicology study of BMS-B in rats. In summary, although the SDD approach could be quite challenging for highly lipophilic compounds because of the limitation on wetting and dissolution, the present study demonstrated that SDD can be applied in drug discovery to enhance oral exposure and enable preclinical toxicology studies of highly lipophilic poorly water-soluble compounds.
authors.affiliation: Discovery Pharmaceutics, Bristol-Myers Squibb, Princeton, New Jersey. Electronic address: xue-qing.chen@bms.com.
authors.forename: Xue-Qing
authors.initials: XQ
authors.lastname: Chen
authors.affiliation: Discovery Pharmaceutics, Bristol-Myers Squibb, Princeton, New Jersey.
authors.forename: Kevin
authors.initials: K
authors.lastname: Stefanski
authors.affiliation: Metabolism and Pharmacokinetics, Bristol-Myers Squibb, Princeton, New Jersey.
authors.forename: Hong
authors.initials: H
authors.lastname: Shen
authors.affiliation: Metabolism and Pharmacokinetics, Bristol-Myers Squibb, Princeton, New Jersey.
authors.forename: Christine
authors.initials: C
authors.lastname: Huang
authors.affiliation: Bioanalytical Sciences, Bristol-Myers Squibb, Pennington, New Jersey.
authors.forename: Christian
authors.initials: C
authors.lastname: Caporuscio
authors.affiliation: Discovery Chemistry, Bristol-Myers Squibb, Pennington, New Jersey.
authors.forename: Wu
authors.initials: W
authors.lastname: Yang
authors.affiliation: Discovery Chemistry, Bristol-Myers Squibb, Pennington, New Jersey.
authors.forename: Patrick
authors.initials: P
authors.lastname: Lam
authors.affiliation: Discovery Pharmaceutics, Bristol-Myers Squibb, Princeton, New Jersey.
authors.forename: Ching
authors.initials: C
authors.lastname: Su
authors.affiliation: Discovery Pharmaceutics, Bristol-Myers Squibb, Princeton, New Jersey.
authors.forename: Olafur
authors.initials: O
authors.lastname: Gudmundsson
authors.affiliation: Discovery Pharmaceutics, Bristol-Myers Squibb, Princeton, New Jersey.
authors.forename: Michael
authors.initials: M
authors.lastname: Hageman
mesh_terms: D000284:Administration, Oral; D000818:Animals; D001682:Biological Availability; D002626:Chemistry, Pharmaceutical / Q000379:methods; D000882:Haplorhini; D008252:Macaca fascicularis; D008297:Male; D008747:Methylcellulose / Q000008:administration & dosage / Q000031:analogs & derivatives / Q000737:chemistry; D058921:Purinergic P2Y Receptor Antagonists / Q000008:administration & dosage* / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012995:Solubility; D012996:Solutions / Q000737:chemistry; D014867:Water
chemical_list: D058921:Purinergic P2Y Receptor Antagonists; D012996:Solutions; D014867:Water; C048331:hydroxypropylmethylcellulose acetate succinate; D008747:Methylcellulose
keywords: absorption; amorphous; dissolution; solubility; spray drying