title: Real-time multianalyte biosensors based on interference-free multichannel monolithic quartz crystal microbalance.
abstract: In this work, we design, fabricate and characterize a new interference-free multichannel monolithic quartz crystal microbalance (MQCM) platform for bio-sensing applications. Firstly, interference due to thickness-shear vibration mode coupling between channels in MQCM array is effectively suppressed by interposing a polydimethylsiloxane wall between adjacent QCM electrodes on a quartz substrate to form inverted-mesa-like structure. In addition, the electrical coupling due to the electrical impedance of solution is diminished by extending the flow path between them with an extended-design flow channel. The electrical testing results show that individual QCM signal is unaffected by those of adjacent channels under liquid loading, signifying the achievement of interference-free MQCM. The MQCM is applied for multi-analyte biosensing of IgG and HSA. The anti-IgG and anti-HSA are separately immobilized on two adjacent QCM electrodes, which are subsequently blocked with BSA to avoid unspecific binding. The MQCM biosensors are tested with single- and double-analyte solutions under continuous flow of buffer. The IgG and HSA QCM sensors only show frequency shift responses to their corresponding analytes and there are very small cross frequency shifts due to remnant unspecific binding. Moreover, MQCM sensors show approximately linear frequency shift response with analyte concentration. Therefore, the developed MQCM platform is promising for real-time interference-free label-free detection and quantification of multiple bio-analytes.
authors.affiliation: Department of Electronics, Faculty of Engineering, King Mongkut's Institute of Technology Ladkrabang (KMITL), Thailand; Nanoelectronics and MEMS Laboratory, National Electronics and Computer Technology Center (NECTEC), National Science and Technology Development Agency (NSTDA), Thailand.
authors.forename: Kata
authors.initials: K
authors.lastname: Jaruwongrungsee
authors.affiliation: Nanoelectronics and MEMS Laboratory, National Electronics and Computer Technology Center (NECTEC), National Science and Technology Development Agency (NSTDA), Thailand.
authors.forename: Uraiwan
authors.initials: U
authors.lastname: Waiwijit
authors.affiliation: Nanoelectronics and MEMS Laboratory, National Electronics and Computer Technology Center (NECTEC), National Science and Technology Development Agency (NSTDA), Thailand.
authors.forename: Anurat
authors.initials: A
authors.lastname: Wisitsoraat
authors.affiliation: Biomedical Engineering Program, Faculty of Science, Rangsit University, Thailand.
authors.forename: Manas
authors.initials: M
authors.lastname: Sangworasil
authors.affiliation: Department of Electronics, Faculty of Engineering, King Mongkut's Institute of Technology Ladkrabang (KMITL), Thailand. Electronic address: chuchartpintavirooj@gmail.com.
authors.forename: Chuchart
authors.initials: C
authors.lastname: Pintavirooj
authors.affiliation: Nanoelectronics and MEMS Laboratory, National Electronics and Computer Technology Center (NECTEC), National Science and Technology Development Agency (NSTDA), Thailand. Electronic address: adisorn.tuantranont@nectec.or.th.
authors.forename: Adisorn
authors.initials: A
authors.lastname: Tuantranont
mesh_terms: D000888:Antibodies, Anti-Idiotypic / Q000737:chemistry / Q000302:isolation & purification*; D015374:Biosensing Techniques / Q000379:methods*; D017097:Electric Impedance; D006801:Humans; D058525:Quartz Crystal Microbalance Techniques / Q000379:methods*; D012709:Serum Albumin / Q000737:chemistry / Q000302:isolation & purification*; D012996:Solutions / Q000737:chemistry
chemical_list: D000888:Antibodies, Anti-Idiotypic; D012709:Serum Albumin; D012996:Solutions; C048844:anti-IgG
keywords: Interference-free; Multi-analyte biosensors; Multichannel monolithic quartz crystal microbalance; Real-time detection