title: Combining Sodium Hyaluronate and Polyvinylpyrrolidone Therapies for the Rabbit Cornea: A New Approach to Relief of the Human Dry Eye Syndrome.
abstract: OBJECTIVE
  The novel combination of 0.1% sodium hyaluronate (HA) and 5.0% polyvinylpyrrolidone (PVP) into one eyedrop was investigated to test the hypothesis of its increased relief of dry eye syndrome (DES).
  
  
  METHODS
  We evaluated HA and PVP, either alone, or in combination, by utilizing 16 rabbits, where their right eyes received one or two different eyedrops, and their left eyes, as controls, received none. The DES replica in rabbits was induced by 0.1% benzalkonium chloride (BAC) eyedrops. BAC was instilled into the right eyes of all rabbits, which were divided into four groups of four. In Group 1 M, the rabbits received only BAC. A second eyedrop given to the right eyes of Group 2 M was HA, of Group 3 M was PVP, and of Group 4 M was the combination of both HA and PVP. All eyes were followed clinically for 14 d, and thereafter, examined histopathologically.
  
  
  RESULTS
  Clinically, the HA+PVP combination yielded the least perilimbal conjunctival erythema (p < 0.05), and the least corneal epithelial fluorescein staining (p < 0.001) compared to each treatment alone. Histopathologically, all four rabbits' right eyes in the combination group 4 M displayed the greatest preservation of the corneal epithelium (p < 0.001) and of the perilimbal conjunctival goblet cell density (p < 0.001).
  
  
  CONCLUSIONS
  This unique combination of both HA and PVP into one eyedrop, was more potent than either treatment alone in protecting the ocular surface. A preparation, containing both HA and PVP may become useful for DES patients.
authors.affiliation: Medicure Technologies Ltd. , Caesarea , Israel and.
authors.forename: Moshe
authors.initials: M
authors.lastname: Ehrenberg
authors.forename: Eyal
authors.initials: E
authors.lastname: Zolotariov
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Loeb
authors.forename: Vadim
authors.initials: V
authors.lastname: Poliansky
authors.forename: Aharon
authors.initials: A
authors.lastname: Levy
mesh_terms: D000276:Adjuvants, Immunologic / Q000008:administration & dosage; D000818:Animals; D003315:Cornea / Q000187:drug effects / Q000378:metabolism / Q000473:pathology*; D004195:Disease Models, Animal; D004359:Drug Therapy, Combination; D015352:Dry Eye Syndromes / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D006801:Humans; D006820:Hyaluronic Acid / Q000008:administration & dosage*; D008297:Male; D009883:Ophthalmic Solutions; D010952:Plasma Substitutes / Q000008:administration & dosage; D011205:Povidone / Q000008:administration & dosage*; D011817:Rabbits; D016896:Treatment Outcome
chemical_list: D000276:Adjuvants, Immunologic; D009883:Ophthalmic Solutions; D010952:Plasma Substitutes; D006820:Hyaluronic Acid; D011205:Povidone
keywords: Conjunctiva; cornea; dry eyes; goblet cells; tears