title: Metaphylogenomic and potential functionality of the limpet Patella pellucida's gastrointestinal tract microbiome.
abstract: This study investigated the microbial diversity associated with the digestive tract of the seaweed grazing marine limpet Patella pellucida. Using a modified indirect DNA extraction protocol and performing metagenomic profiling based on specific prokaryotic marker genes, the abundance of bacterial groups was identified from the analyzed metagenome. The members of three significantly abundant phyla of Proteobacteria, Firmicutes and Bacteroidetes were characterized through the literature and their predicted functions towards the host, as well as potential applications in the industrial environment assessed.
authors.affiliation: Institute of Biological, Environmental & Rural Sciences (IBERS), Aberystwyth University, Gogerddan, Aberystwyth, Ceredigion, Wales SY23 3EE, UK. mad27@aber.ac.uk.
authors.forename: Magda
authors.initials: M
authors.lastname: Dudek
authors.affiliation: Institute of Biological, Environmental & Rural Sciences (IBERS), Aberystwyth University, Gogerddan, Aberystwyth, Ceredigion, Wales SY23 3EE, UK. jaa@aber.ac.uk.
authors.forename: Jessica
authors.initials: J
authors.lastname: Adams
authors.affiliation: Institute of Biological, Environmental & Rural Sciences (IBERS), Aberystwyth University, Gogerddan, Aberystwyth, Ceredigion, Wales SY23 3EE, UK. mts11@aber.ac.uk.
authors.forename: Martin
authors.initials: M
authors.lastname: Swain
authors.affiliation: Institute of Biological, Environmental & Rural Sciences (IBERS), Aberystwyth University, Gogerddan, Aberystwyth, Ceredigion, Wales SY23 3EE, UK. ayh@aber.ac.uk.
authors.forename: Matthew
authors.initials: M
authors.lastname: Hegarty
authors.affiliation: Institute of Biological, Environmental & Rural Sciences (IBERS), Aberystwyth University, Gogerddan, Aberystwyth, Ceredigion, Wales SY23 3EE, UK. hnh@aber.ac.uk.
authors.forename: Sharon
authors.initials: S
authors.lastname: Huws
authors.affiliation: Institute of Biological, Environmental & Rural Sciences (IBERS), Aberystwyth University, Gogerddan, Aberystwyth, Ceredigion, Wales SY23 3EE, UK. jbg@aber.ac.uk.
authors.forename: Joe
authors.initials: J
authors.lastname: Gallagher
mesh_terms: D000818:Animals; D001419:Bacteria / Q000235:genetics* / Q000302:isolation & purification*; D001439:Bacteroides / Q000235:genetics / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D041981:Gastrointestinal Tract / Q000382:microbiology; D049851:Gastropoda / Q000382:microbiology*; D054892:Metagenome; D056186:Metagenomics; D064307:Microbiota; D010802:Phylogeny; D020560:Proteobacteria / Q000235:genetics / Q000302:isolation & purification
chemical_list: D004269:DNA, Bacterial