title: Mouse models of osteoarthritis: surgical model of posttraumatic osteoarthritis induced by destabilization of the medial meniscus.
abstract: The surgical model of destabilization of the medial meniscus (DMM) has become a gold standard for studying the onset and progression of posttraumatic osteoarthritis (OA). The DMM model mimics clinical meniscal injury, a known predisposing factor for the development of human OA, and permits the study of structural and biological changes over the course of the disease. In addition, when applied to genetically modified or engineered mouse models, this surgical procedure permits dissection of the relative contribution of a given gene to OA initiation and/or progression. This chapter describes the requirements for the surgical induction of OA in mouse models, and provides guidelines and tools for the subsequent histological, immunohistochemical, and molecular analyses. Methods for the assessment of the contributions of selected genes in genetically modified strains are also provided.
authors.affiliation: Tissue Engineering Regeneration and Repair Program, Research Division, The Hospital for Special Surgery, Caspary Research Building, 5th Floor, 535 East 70th Street, New York, NY, 10021, USA, CulleyK@hss.edu.
authors.forename: Kirsty L
authors.initials: KL
authors.lastname: Culley
authors.forename: Cecilia L
authors.initials: CL
authors.lastname: Dragomir
authors.forename: Jun
authors.initials: J
authors.lastname: Chang
authors.forename: Elisabeth B
authors.initials: EB
authors.lastname: Wondimu
authors.forename: Jonathan
authors.initials: J
authors.lastname: Coico
authors.forename: Darren A
authors.initials: DA
authors.lastname: Plumb
authors.forename: Miguel
authors.initials: M
authors.lastname: Otero
authors.forename: Mary B
authors.initials: MB
authors.lastname: Goldring
mesh_terms: D000818:Animals; D001169:Arthritis, Experimental / Q000473:pathology / Q000503:physiopathology; D006801:Humans; D007718:Knee Injuries / Q000150:complications / Q000473:pathology / Q000503:physiopathology; D008592:Menisci, Tibial / Q000473:pathology / Q000503:physiopathology; D051379:Mice; D020370:Osteoarthritis, Knee / Q000209:etiology / Q000473:pathology / Q000503:physiopathology; D000070600:Tibial Meniscus Injuries