title: The SNAI1 3'UTR functions as a sponge for multiple migration-/invasion-related microRNAs.
abstract: Accumulating evidence has indicated a large-scale regulatory network generated by 3'untranslated regions (3'UTRs) in cancer. The 3'UTRs act not only in cis but, most likely even more importantly, as trans regulators of gene expression, consequently leading to phenotypic alterations. Here, we found that ectopic expression of SNAI1 3'UTR induced migration and invasion of ovarian cancer cell line RMUG-L without significantly affecting cell viability. Additionally, SNAI1 3'UTR overexpression regulated key epithelial-to-mesenchymal transition (EMT) markers, including SNAI1, Vimentin, and E-cadherin, and functioned as a sponge for multiple migration-/invasion-related microRNAs (miRNAs) in RMUG-L cells. These findings revealed the noncoding function of SNAI1 for the first time.
authors.affiliation: Department of Gynecology, Obstetrics and Gynecology Hospital, Fudan University, No. 419, Fangxie Road, Shanghai, 200011, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Li
authors.forename: Hailin
authors.initials: H
authors.lastname: Yu
authors.forename: Meili
authors.initials: M
authors.lastname: Xi
authors.forename: Duan
authors.initials: D
authors.lastname: Ma
authors.forename: Xin
authors.initials: X
authors.lastname: Lu
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics*; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D002470:Cell Survival; D058750:Epithelial-Mesenchymal Transition / Q000235:genetics; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D053263:Gene Regulatory Networks; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D009361:Neoplasm Invasiveness / Q000235:genetics; D010051:Ovarian Neoplasms / Q000235:genetics* / Q000473:pathology; D000071250:Snail Family Transcription Factors; D014157:Transcription Factors / Q000096:biosynthesis / Q000235:genetics*; D014746:Vimentin / Q000235:genetics
chemical_list: D020413:3' Untranslated Regions; D035683:MicroRNAs; C000604407:SNAI1 protein, human; D000071250:Snail Family Transcription Factors; D014157:Transcription Factors; D014746:Vimentin