title: Chondrogenic differentiation of ChM-I gene transfected rat bone marrow-derived mesenchymal stem cells on 3-dimensional poly (L-lactic acid) scaffold for cartilage engineering.
abstract: We have explored the role of Chondromodulin-I (ChM-I) in chondrogenesis of bone marrow-derived mesenchymal stem cells (BMSCs) in 3-dimensional (3D) scaffold for cartilage tissue engineering. BMSCs of Sprague Dawley (SD) rats were cultured on poly-(L-lactic acid) [PLLA] scaffolds with different pore sizes (80-200 μm, 200-450 μm) with or without surface modification by chitosan. Cell viability, proliferation, and morphology were measured using confocal microscope and the CCK-8 method. Untransfected BMSCs, BMSCs expressing pcDNA3.1(+), BMSCs expressing plasmid pcDNA3.1 (+)/ChM-I were cultured on 3D scaffolds in standard growth medium or transforming growth factor-β1 (TGF-β1) supplemented chondrogenic induction medium in vitro for 3 weeks and the expression of collagen type II was determined. Cell-scaffolds constructs were implanted subcutaneously for 3 months in vivo. BMSCs had a higher viability and proliferation in PLLA scaffolds of pore size 200-450 μm than that of 80-200 μm, and surface modification with chitosan did not enhance cell attachment. The ChM-I gene enhanced chondrogenesis and increased collagen type II synthesis. Immunohistochemistry from in vivo study showed enhanced cartilage regeneration in BMSCs expressing pcDNA3.1 (+)/ChM-I on 3D PLLA scaffolds. It also demonstrated that TGF-β1 might promote chondrogenesis of rat BMSCs by synergizing with the ChM-I gene. ChM-I could be beneficial to future applications in cartilage repair.
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, the First Affiliated Hospital of Dalian Medical University, Dalian, 116011, P.R. China.
authors.forename: Shuang-Chun
authors.initials: SC
authors.lastname: Xing
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.forename: Y
authors.initials: Y
authors.lastname: Feng
authors.forename: Chen
authors.initials: C
authors.lastname: Jiang
authors.forename: Yu-Qiang
authors.initials: YQ
authors.lastname: Hu
authors.forename: Wei
authors.initials: W
authors.lastname: Sun
authors.forename: Xin-Hui
authors.initials: XH
authors.lastname: Wang
authors.forename: Zhi-Yong
authors.initials: ZY
authors.lastname: Wei
authors.forename: Min
authors.initials: M
authors.lastname: Qi
authors.forename: Jing
authors.initials: J
authors.lastname: Liu
authors.forename: Li-Jie
authors.initials: LJ
authors.lastname: Zhai
authors.forename: Zhi-Qiang
authors.initials: ZQ
authors.lastname: Wang
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology*; D002356:Cartilage / Q000378:metabolism / Q000502:physiology; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation; D002478:Cells, Cultured; D020219:Chondrogenesis / Q000187:drug effects; D024043:Collagen Type II / Q000378:metabolism; D036341:Intercellular Signaling Peptides and Proteins / Q000235:genetics / Q000378:metabolism*; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism*; D011107:Polylysine / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012038:Regeneration / Q000187:drug effects; D023822:Tissue Engineering; D054457:Tissue Scaffolds; D014162:Transfection; D053773:Transforming Growth Factor beta1 / Q000494:pharmacology
chemical_list: C489407:Cnmd protein, rat; D024043:Collagen Type II; D036341:Intercellular Signaling Peptides and Proteins; D008565:Membrane Proteins; D053773:Transforming Growth Factor beta1; D011107:Polylysine
keywords: ChM-I gene; cartilage tissue engineering; poly- (L-lactic acid); rat BMSCs