title: Fermented soy permeate reduces cytokine level and oxidative stress in streptozotocin-induced diabetic rats.
abstract: Oxidative stress and inflammation are involved in the development of type 1 diabetes and its complications. Because two compounds found in soy, that is, isoflavones and alpha-galactooligosaccharides, have been shown to exert antioxidant and anti-inflammatory effects, this study aimed to assess the effects of a dietary supplement containing these two active compounds, the fermented soy permeate (FSP). We hypothesized that FSP would be able to reduce in vivo oxidative stress and inflammation in streptozotocin (STZ)-induced type 1 diabetic rats. Thirty male Wistar rats were divided into the control placebo, diabetic placebo, and diabetic FSP-supplemented groups. They received daily, by oral gavage, water (placebo groups) or diluted FSP (0.1 g/day; FSP-supplemented group). After 3 weeks, glycemic regulation (glycemia and fructosamine level); the plasma level of carboxymethyllysine (CML), a marker of systemic oxidative stress in diabetes; and the plasma levels of inflammatory markers (CRP, IL-1β, IL-6, and uric acid) were evaluated. Markers of oxidative damage (isoprostanes and GSH/GSSG), antioxidant enzymatic activity (SOD and GPX), and Mn-SOD content were determined in skeletal muscle (gastrocnemius). Diabetic placebo rats exhibited higher CML levels, lower SOD and GPX activities, and decreased Mn-SOD contents. FSP supplementation in diabetic animals normalized the CML and antioxidant enzymatic activity levels and tended to increase Mn-SOD expression. The markers of inflammation whose levels were increased in the diabetic placebo group were markedly decreased by FSP (IL-1β: -75%, IL-6: -46%, and uric acid: -17%), except for CRP. Our results demonstrate that FSP exhibited antioxidant and anti-inflammatory properties in vivo in STZ-induced diabetic rats.
authors.affiliation: 1 Laboratory M2S, Université Rennes 2-ENS Cachan , Rennes, France .
authors.forename: Ludivine
authors.initials: L
authors.lastname: Malardé
authors.forename: Carole
authors.initials: C
authors.lastname: Groussard
authors.forename: Luz
authors.initials: L
authors.lastname: Lefeuvre-Orfila
authors.forename: Sophie
authors.initials: S
authors.lastname: Vincent
authors.forename: Théo
authors.initials: T
authors.lastname: Efstathiou
authors.forename: Arlette
authors.initials: A
authors.lastname: Gratas-Delamarche
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000378:metabolism / Q000494:pharmacology / Q000627:therapeutic use*; D000975:Antioxidants / Q000378:metabolism / Q000494:pharmacology / Q000627:therapeutic use; D015415:Biomarkers / Q000097:blood; D003921:Diabetes Mellitus, Experimental / Q000097:blood / Q000150:complications / Q000188:drug therapy; D003922:Diabetes Mellitus, Type 1 / Q000097:blood / Q000150:complications / Q000188:drug therapy*; D019587:Dietary Supplements; D005285:Fermentation; D005690:Galactose / Q000494:pharmacology / Q000627:therapeutic use; D007249:Inflammation / Q000188:drug therapy / Q000209:etiology / Q000378:metabolism; D007378:Interleukins / Q000097:blood*; D007529:Isoflavones / Q000494:pharmacology / Q000627:therapeutic use*; D028421:Isoprostanes / Q000097:blood; D008239:Lysine / Q000031:analogs & derivatives / Q000097:blood; D008297:Male; D009844:Oligosaccharides / Q000494:pharmacology / Q000627:therapeutic use*; D018384:Oxidative Stress / Q000187:drug effects*; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use; D017208:Rats, Wistar; D013025:Glycine max / Q000737:chemistry*; D013482:Superoxide Dismutase / Q000378:metabolism; D014527:Uric Acid / Q000097:blood
chemical_list: D000893:Anti-Inflammatory Agents; D000975:Antioxidants; D015415:Biomarkers; D007378:Interleukins; D007529:Isoflavones; D028421:Isoprostanes; D009844:Oligosaccharides; D010936:Plant Extracts; D014527:Uric Acid; C048496:N(6)-carboxymethyllysine; D013482:Superoxide Dismutase; D008239:Lysine; D005690:Galactose
keywords: alpha-galactooligosaccharides; inflammation; oxidative stress; rat; soy isoflavones; type 1 diabetes