title: Profiling histone modifications by chromatin immunoprecipitation coupled to deep sequencing in skeletal cells.
abstract: Chromatin, tightly packaged genomic DNA, is reliant on posttranslational modification of histone N-terminal tails for accessibility of DNA by transcription factors to activate transcription. Each histone modification may denote permissible states for gene activation or repression. As cells undergo differentiation, as they do in the skeleton from multipotential precursors through osteoblasts and into osteocytes, their histone code may be altered to help accommodate these transitions. Here we describe the methodology of chromatin immunoprecipitation (ChIP) coupled to deep sequencing (ChIP-seq) on skeletal cells that have differentiated in cell culture.
authors.affiliation: Department of Biochemistry, University of Wisconsin, 440 Henry Mall, Madison, WI, 53706, USA.
authors.forename: Mark B
authors.initials: MB
authors.lastname: Meyer
authors.forename: Nancy A
authors.initials: NA
authors.lastname: Benkusky
authors.forename: J Wesley
authors.initials: JW
authors.lastname: Pike
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000502:physiology*; D002460:Cell Line; D047369:Chromatin Immunoprecipitation / Q000379:methods*; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods*; D006657:Histones / Q000378:metabolism*; D051379:Mice; D018485:Muscle Fibers, Skeletal / Q000166:cytology / Q000378:metabolism*; D011499:Protein Processing, Post-Translational / Q000502:physiology*
chemical_list: D006657:Histones