title: Tissue bioavailability of anthocyanins from whole tart cherry in healthy rats.
abstract: Our aim was to confirm and identify the presence of tart cherry anthocyanins in several target tissues of healthy rats. Liquid chromatography-mass spectrometry analysis was employed for detection and characterisation of anthocyanin metabolites. It was shown that four native anthocyanins, namely cyanidin 3-glucosylrutinoside, cyanidin 3-rutinoside, cyanidin 3-rutinoside 5-β-D-glucoside, and peonidin 3-rutinoside were differentially distributed among targeted tissues of rats. Bladder and kidney contained more total anthocyanins than all other tissues analysed. It was also revealed that the bioavailability pattern of these native anthocyanins among tissues is varied. The highest concentration of individual anthocyanin cyanidin 3-glucosylrutinoside (2339 picograms/gram of tissue) was detected in bladder, followed by cyanidin 3-rutinoside 5-β-d-glucoside (916 picograms/gram) in the liver of rats. Although the diverse distribution of tart cherry anthocyanins in different rat tissues still requires further explanation, it may provide an evidentiary link between tissue bioavailability and health-enhancing properties of anthocyanins at target sites.
authors.affiliation: University of Michigan Health System, Cardiovascular Center and the Michigan Integrative Medicine Program, Ann Arbor, MI 48109, United States. Electronic address: akirakos@umich.edu.
authors.forename: Ara
authors.initials: A
authors.lastname: Kirakosyan
authors.affiliation: University of Michigan Health System, Cardiovascular Center and the Michigan Integrative Medicine Program, Ann Arbor, MI 48109, United States.
authors.forename: E Mitchell
authors.initials: EM
authors.lastname: Seymour
authors.affiliation: University of Michigan Health System, Cardiovascular Center and the Michigan Integrative Medicine Program, Ann Arbor, MI 48109, United States.
authors.forename: Janet
authors.initials: J
authors.lastname: Wolforth
authors.affiliation: University of Michigan Health System, Cardiovascular Center and the Michigan Integrative Medicine Program, Ann Arbor, MI 48109, United States.
authors.forename: Robert
authors.initials: R
authors.lastname: McNish
authors.affiliation: University of Michigan Health System, Cardiovascular Center and the Michigan Integrative Medicine Program, Ann Arbor, MI 48109, United States.
authors.forename: Peter B
authors.initials: PB
authors.lastname: Kaufman
authors.affiliation: University of Michigan Health System, Cardiovascular Center and the Michigan Integrative Medicine Program, Ann Arbor, MI 48109, United States.
authors.forename: Steven F
authors.initials: SF
authors.lastname: Bolling
mesh_terms: D000818:Animals; D000872:Anthocyanins / Q000302:isolation & purification / Q000493:pharmacokinetics*; D001682:Biological Availability; D007668:Kidney / Q000737:chemistry / Q000378:metabolism*; D008297:Male; D009928:Organ Specificity; D027861:Prunus / Q000737:chemistry*; D051381:Rats; D017208:Rats, Wistar; D014018:Tissue Distribution; D001743:Urinary Bladder / Q000737:chemistry / Q000378:metabolism*
chemical_list: D000872:Anthocyanins
keywords: Anthocyanins; Bioavailability; Rats; Tart cherry; Tissue distribution