title: A novel device to concurrently assess leukocyte extravasation and interstitial migration within a defined 3D environment.
abstract: Leukocyte extravasation and interstitial migration are key events during inflammation. Traditional in vitro techniques address only specific steps of cell recruitment to tissues and fail to recapitulate the whole process in an appropriate three-dimensional (3D) microenvironment. Herein, we describe a device that enables us to qualitatively and quantitatively assess in 4D the interdependent steps underlying leukocyte trafficking in a close-to-physiology in vitro context. Real-time tracking of cells, from initial adhesion to the endothelium and subsequent diapedesis to interstitial migration towards the source of the chemoattractant within the 3D collagen matrix, is enabled by the use of optically transparent porous membranes laid over the matrix. Unique features of the device, such as the use of non-planar surfaces and the contribution of physiological flow to the establishment of a persistent chemoattractant gradient, were assessed by numerical simulations and validated by proof-of-concept, simultaneous testing of differentially treated primary mouse neutrophils. This microfluidic platform offers new and versatile tools to thoroughly investigate the stepwise process of circulating cell recruitment to target tissues in vitro and to test novel therapeutics targeting various steps of the process.
authors.affiliation: Division of Immunology, Transplantation and Infectious Diseases, Leukocyte Biology Unit, San Raffaele Scientific Institute, Milan, Italy. pardi.ruggero@hsr.it.
authors.forename: Raffaella
authors.initials: R
authors.lastname: Molteni
authors.forename: Elena
authors.initials: E
authors.lastname: Bianchi
authors.forename: Paolo
authors.initials: P
authors.lastname: Patete
authors.forename: Monica
authors.initials: M
authors.lastname: Fabbri
authors.forename: Guido
authors.initials: G
authors.lastname: Baroni
authors.forename: Gabriele
authors.initials: G
authors.lastname: Dubini
authors.forename: Ruggero
authors.initials: R
authors.lastname: Pardi
mesh_terms: D000818:Animals; D002465:Cell Movement / Q000502:physiology*; D002478:Cells, Cultured; D003198:Computer Simulation; D003584:Cytological Techniques / Q000295:instrumentation* / Q000379:methods; D004867:Equipment Design; D007962:Leukocytes / Q000166:cytology* / Q000502:physiology; D051379:Mice; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods; D008954:Models, Biological