title: An orthogonal photolabile linker for the complete "on-support" synthesis/fast deprotection/hybridization of RNA.
abstract: The preparation of a polystyrene solid support decorated with a photolabile linker is described. The entire post-synthetic processing of RNA can be carried out in the solid phase in a minimum amount of time. The deprotected RNA is available for "on-support" hybridization and photolysis releases siRNA duplexes under mild, neutral conditions.
authors.affiliation: Department of Chemistry, McGill University, Montréal, Québec H3A 0B8, Canada. masad.damha@mcgill.ca.
authors.forename: Jory
authors.initials: J
authors.lastname: Lietard
authors.forename: Matthew R
authors.initials: MR
authors.lastname: Hassler
authors.forename: Johans
authors.initials: J
authors.lastname: Fakhoury
authors.forename: Masad J
authors.initials: MJ
authors.lastname: Damha
mesh_terms: D055785:Gene Knockdown Techniques; D006367:HeLa Cells; D006801:Humans; D049409:Luciferases, Firefly / Q000235:genetics; D009693:Nucleic Acid Hybridization; D009841:Oligonucleotides / Q000737:chemistry; D010782:Photolysis; D011137:Polystyrenes / Q000737:chemistry; D012313:RNA / Q000737:chemistry*; D014466:Ultraviolet Rays
chemical_list: D009841:Oligonucleotides; D011137:Polystyrenes; D012313:RNA; D049409:Luciferases, Firefly