title: Phytochemical and biological activities of Bituminaria bituminosa L. (Fabaceae).
abstract: OBJECTIVE
  To investigate the phytochemical composition, the antioxidant and antibacterial activities of Bituminaria bituminosa L. (Fabaceae) (B. bituminosa).
  
  
  METHODS
  The aerial parts of B. bituminosa yielded two compounds. The structures of these compounds were determinated using UV, (1)H-NMR and (13)C-NMR experiments and comparison of their spectroscopic properties with literature data. The antibacterial activity of the extracts (CH2Cl2, ethyl acetate and n-BuOH) was determinated using disk diffusion method against standard and clinical strains. Antioxidant potential of n-BuOH extract was evaluated through two methods: DPPH and cupric ion reducing antioxidant capacity assay.
  
  
  RESULTS
  The n-BuOH extract from B. bituminosa yielded the isolation of isoflavone and flavone. The extracts CH2Cl2, ethyl acetate and n-BuOH demonstrated significant antibacterial activities. CH2Cl2 extract showed the maximum antibacterial activity with high concentration of 2 mg/mL against Staphylococcus aureus ATCC 29213, Klebsiella pneumonia and Escherichia coli ATCC 25922 (20.45 mm, 16.41 mm and 15.74 mm inhibition zone, respectively). The value IC50 was 0.26 μg/mL for n-BuOH extract using DPPH method. Whereas the E% value was 0.10 L/mg every centimeter for cupric ion reducing antioxidant capacity assay.
  
  
  CONCLUSIONS
  The phytochemical study of B. bituminosa revealed the presence of isoflavone (daidzin) and flavone (isoorientin) and identified for the first time in this specie. The antibacterial activity of the plant B. bituminosa is certainly related to its chemical content. The n-BuOH extract showed a significant antioxidant activity.
authors.affiliation: Unité de Recherche Valorisation des Ressources Naturelles Molécules Bioactives et Analyses Physico-Chimiques et Biologiques, Departement de Chimie, Facultés des Sciences Exactes, Université de Constantine 1, Algérie.
authors.forename: Salima
authors.initials: S
authors.lastname: Azzouzi
authors.affiliation: Unité de Recherche Valorisation des Ressources Naturelles Molécules Bioactives et Analyses Physico-Chimiques et Biologiques, Departement de Chimie, Facultés des Sciences Exactes, Université de Constantine 1, Algérie.
authors.forename: Nabila
authors.initials: N
authors.lastname: Zaabat
authors.affiliation: Unité de Recherche Valorisation des Ressources Naturelles Molécules Bioactives et Analyses Physico-Chimiques et Biologiques, Departement de Chimie, Facultés des Sciences Exactes, Université de Constantine 1, Algérie.
authors.forename: Kamel
authors.initials: K
authors.lastname: Medjroubi
authors.affiliation: Unité de Recherche Valorisation des Ressources Naturelles Molécules Bioactives et Analyses Physico-Chimiques et Biologiques, Departement de Chimie, Facultés des Sciences Exactes, Université de Constantine 1, Algérie. Electronic address: salah4dz@yahoo.fr.
authors.forename: Salah
authors.initials: S
authors.lastname: Akkal
authors.affiliation: Laboratoire de Bactóriologie CHU, Benbadis Constantine, Algórie.
authors.forename: Kadour
authors.initials: K
authors.lastname: Benlabed
authors.affiliation: Laboratoire de Bactóriologie CHU, Benbadis Constantine, Algórie.
authors.forename: Farida
authors.initials: F
authors.lastname: Smati
authors.affiliation: UMR 5557, Ecologie Microbienne, ISPB, 69373 Lyon, France.
authors.forename: Marie-Geneviève
authors.initials: MG
authors.lastname: Dijoux-Franca
keywords: Antibacterial; Antioxidant; Bituminaria bituminosa L.; Escherichia coli; Fabaceae; Flavone; Isoflavone; Klebsiella pneumonia; Staphylococcus aureus