title: Screening of Rubiaceae and Apocynaceae extracts for mosquito larvicidal potential.
abstract: Rubiaceae and Apocynaceae families are well known for the expression of cyclotides having insecticidal properties. Leaves and flowers extracts of plants from the families Rubiaceae (Ixora coccinea) and Apocynaceae (Allamanda violacea) were evaluated for mosquito larvicidal effect against early IVth instars of Aedes aegypti and Anopheles stephensi. Two forms of plant extracts, one untreated and the other treated with heat and proteolytic enzyme were used for assay. After primary assay, the extract showing more than 50% inhibition was further used for quantification purpose. LC50 and LC90 values of all the extracts were found to be reduced with the treated form. Phytochemical analysis of plant extracts was performed. Primary confirmation for the presence of cyclotides was done by Lowry test, thin layer chromatography and haemolytic assay. This novel approach merits use of plant extracts in mosquito control programmes.
authors.affiliation: a School of Life Sciences, North Maharashtra University , Jalgaon 425001 , Maharashtra , India.
authors.forename: Rahul
authors.initials: R
authors.lastname: Suryawanshi
authors.forename: Chandrashekhar
authors.initials: C
authors.lastname: Patil
authors.forename: Hemant
authors.initials: H
authors.lastname: Borase
authors.forename: Chandrakant
authors.initials: C
authors.lastname: Narkhede
authors.forename: Satish
authors.initials: S
authors.lastname: Patil
mesh_terms: D000330:Aedes; D000818:Animals; D000852:Anopheles; D029062:Apocynaceae / Q000737:chemistry*; D035264:Flowers / Q000737:chemistry; D007306:Insecticides; D007814:Larva; D009032:Mosquito Control; D010936:Plant Extracts / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry; D019666:Rubiaceae / Q000737:chemistry*
chemical_list: D007306:Insecticides; D010936:Plant Extracts
keywords: Aedes aegypti; Anopheles stephensi; Apocynaceae; Rubiaceae; cyclotides; larvicidal