title: A sample-to-result system for blood coagulation tests on a microfluidic disk analyzer.
abstract: In this report, we describe in detail a microfluidic analyzer, which is able to conduct blood coagulation tests using whole blood samples. Sample preparation steps, such as whole blood aliquoting and metering, plasma separation, decanting, and mixing with reagents were performed in sequence through microfluidic functions integrated on a disk. Both prothrombin time (PT) and activated partial thromboplastin time (aPTT) were carried out on the same platform and the test results can be reported in 5 min. Fifty clinical samples were tested for both PT and aPTT utilizing the microfluidic disk analyzer and the instrument used in hospitals. The test results showed good correlation and agreement between the two instruments.
authors.affiliation: Shaoxing Pushkang Biotechnology Co., Ltd. , No. 398, Mahuan Road, Binhai New Area, Shaoxing, Zhejiang Province 312366, People's Republic of China.
authors.forename: Chia-Hui
authors.initials: CH
authors.lastname: Lin
authors.affiliation: Department of Chemical Engineering, Feng Chia University , 100 Wenhwa Road, Taichung 40724, Taiwan.
authors.forename: Cheng-Yuan
authors.initials: CY
authors.lastname: Liu
authors.affiliation: Department of Chemical Engineering, Feng Chia University , 100 Wenhwa Road, Taichung 40724, Taiwan.
authors.forename: Chih-Hsin
authors.initials: CH
authors.lastname: Shih
authors.forename: Chien-Hsing
authors.initials: CH
authors.lastname: Lu