title: Alterations of rat stomach endocrine cells under renovascular hypertension.
abstract: OBJECTIVE
  The aim of the present study was to perform immunohistochemical and ultrastructural analysis of gastrin-, synaptophysin (SY)- and atrial natriuretic peptide (ANP)-positive cells in the pylorus of "two kidney, one clip" (2K1C) renovascular hypertension model in rats.
  
  
  METHODS
  In order to identify neuroendocrine (NE) cells, immunohistochemical reactions were performed with the use of specific antibodies against gastrin, SY and ANP. Gastric NE cells were also examined using an electron microscope.
  
  
  RESULTS
  The present study revealed a twofold increase in the number of gastrin- and SY-positive cells and a significant decrease in the number of ANP-immunoreactive (IR) cells in the pyloric mucosa of 2K1C rats. Test results obtained with an electron microscope confirmed a change in the activity of the stomach endocrine cells of hypertensive rats.
  
  
  CONCLUSIONS
  Immunohistochemical and ultrastructural investigations demonstrated the impact of renovascular hypertension on the neuroendocrine system in the rat stomach. The changes in the total number and ultrastructure of DNES cells proved their undeniable role in the modulation of gastric dysfunction, as a consequence of deregulation of homeostasis-maintaining systems.
authors.affiliation: Department of Histology and Cytophysiology, Medical University of Bialystok, Poland. Electronic address: kasacka@umb.edu.pl.
authors.forename: Irena
authors.initials: I
authors.lastname: Kasacka
authors.affiliation: Department of Histology and Cytophysiology, Medical University of Bialystok, Poland.
authors.forename: Zaneta
authors.initials: Z
authors.lastname: Piotrowska
authors.affiliation: Department of Histology and Cytophysiology, Medical University of Bialystok, Poland.
authors.forename: Alicja
authors.initials: A
authors.lastname: Lewandowska
mesh_terms: D000818:Animals; D009320:Atrial Natriuretic Factor / Q000378:metabolism; D015415:Biomarkers / Q000378:metabolism; D004195:Disease Models, Animal; D019858:Enteroendocrine Cells / Q000378:metabolism / Q000648:ultrastructure*; D005753:Gastric Mucosa / Q000378:metabolism / Q000648:ultrastructure*; D005755:Gastrins / Q000378:metabolism; D006978:Hypertension, Renovascular / Q000378:metabolism / Q000473:pathology*; D008297:Male; D011708:Pylorus / Q000378:metabolism / Q000648:ultrastructure*; D017208:Rats, Wistar; D016708:Synaptophysin / Q000378:metabolism
chemical_list: D015415:Biomarkers; D005755:Gastrins; D016708:Synaptophysin; C569016:Syp protein, rat; C472610:atrial natriuretic peptide, rat; D009320:Atrial Natriuretic Factor
keywords: Atrial natriuretic peptide; Gastrin; Renovascular hypertension; Stomach; Synaptophysin