title: miR-CATCH: microRNA capture affinity technology.
abstract: Several experimental methods exist to explore the microRNA (miRNA) regulome. These methods almost exclusively focus on multiple targets bound to a single, or perhaps a few miRNAs of interest. Here, we describe a microRNA capture affinity technology (miR-CATCH) which uses an affinity capture oligonucleotide to co-purify a single target messenger RNA (mRNA) together with all its endogenously bound miRNAs. This bench-top method is similar to RNA immunoprecipitation (RIP) and provides an experimental alternative to computational miRNA target prediction.
authors.affiliation: Respiratory Research Division, Department of Medicine, Royal College of Surgeons in Ireland, Education and Research Centre, Beaumont Hospital, Beaumont Road, Dublin 9, Ireland.
authors.forename: Sebastian
authors.initials: S
authors.lastname: Vencken
authors.forename: Tidi
authors.initials: T
authors.lastname: Hassan
authors.forename: Noel G
authors.initials: NG
authors.lastname: McElvaney
authors.forename: Stephen G J
authors.initials: SG
authors.lastname: Smith
authors.forename: Catherine M
authors.initials: CM
authors.lastname: Greene
mesh_terms: D020029:Base Pairing; D001483:Base Sequence; D002460:Cell Line; D006801:Humans; D035683:MicroRNAs / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization / Q000379:methods*; D016376:Oligonucleotides, Antisense / Q000138:chemical synthesis / Q000235:genetics; D012333:RNA, Messenger / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D035683:MicroRNAs; D016376:Oligonucleotides, Antisense; D012333:RNA, Messenger