title: [Protection of hypothermic preserved isolated rat hearts by resveratrol and its underlying mechanism].
abstract: OBJECTIVE
  To investigate whether resveratrol (RES) plays a protective role in hypothermic preserved isolated rat hearts and whether it is mediated by regulation of silent information regulator protein-1 (Sirt-1) expression.
  
  
  METHODS
  The Langendorff model of isolated rat heart was used. After stored in different Celsior solution at 4 degrees C for 9 h, SD rat hearts were randomly divided into 7 groups: blank control group;9 h group (soley hypothermic preservation for 9 h); RES group (3, 10, 30 micromol/L RES treatment plus hypothermic preservation for 9 h ), niacinamide (NAM) group (40 micromol/L NAM added in Celsior solution plus hypothermic preservation for 9 h), RES + NAM group (30 micromol/L RES and 40 micromol/L NAM were added in Celsior solution plus hypothermic preservation for 9 h). The morphological changes of cardiomyocytes were detected by the HE staining with the light microscope. The mRNA and protein expression levels of Sirt-1 were detected by Real-Time PCR and Western blot respectively.
  
  
  RESULTS
  (1) Compared with the blank control group, myocardiocytes were injured remarkably in the 9 h group and the Sirt-1 mRNA and protein expression levels were decreased significantly (P < 0.01); (2) Compared with the 9 h group, rat myocardial injury was alleviated gradually in 3, 10, 30 micromol/L RES group and the Sirt-1 mRNA and protein expression levels were increased in a dose-dependent manner (P < 0.05); (3) The above protective effects of RES were attenuated by Sirt-1 inhibitor NAM.
  
  
  CONCLUSIONS
  RES can protect myocardiocytes from injury caused by long range hypothermic preservation and this protective effect maybe mediated by upregulation of Sirt-1 expression.
authors.forename: Wei-Ming
authors.initials: WM
authors.lastname: Sun
authors.forename: Ming-Zhi
authors.initials: MZ
authors.lastname: Zheng
authors.forename: Lei
authors.initials: L
authors.lastname: Ying
authors.forename: Xiao-Ming
authors.initials: XM
authors.lastname: Yu
authors.forename: Si-Wen
authors.initials: SW
authors.lastname: Wu
authors.forename: Ying-Ying
authors.initials: YY
authors.lastname: Chen
authors.forename: Yue-Liang
authors.initials: YL
authors.lastname: Shen
authors.forename: Yang
authors.initials: Y
authors.lastname: Wang
mesh_terms: D000818:Animals; D015925:Cryopreservation; D006321:Heart / Q000187:drug effects*; D008297:Male; D009926:Organ Preservation; D051381:Rats; D017207:Rats, Sprague-Dawley; D000077185:Resveratrol; D056564:Sirtuin 1 / Q000378:metabolism*; D013267:Stilbenes / Q000494:pharmacology*
chemical_list: D013267:Stilbenes; C488368:Sirt1 protein, rat; D056564:Sirtuin 1; D000077185:Resveratrol