title: Connection between absorption properties and conformational changes in Deinococcus radiodurans phytochrome.
abstract: Phytochromes consist of several protein domains and a linear tetrapyrrole molecule, which interact as a red-light-sensing system. In this study, size-exclusion chromatography and light-scattering techniques are combined with UV-vis spectroscopy to investigate light-induced changes in dimeric Deinococcus radiodurans bacterial phytochrome (DrBphP) and its subdomains. The photosensory unit (DrCBD-PHY) shows an unusually stable Pfr state with minimal dark reversion, whereas the histidine kinase (HK) domain facilitates dark reversion to the resting state. Size-exclusion chromatography reveals that all phytochrome fragments remain as dimers in the illuminated state and dark state. Still, the elution profiles of all phytochrome fragments differ between the illuminated and dark states. The differences are observed reliably only when the whole UV-vis spectrum is characterized along the elution profile and show more Pfr-state characteristics at later elution volumes in DrBphP and DrCBD-PHY fragments. This implies that the PHY domain has an important role in amplifying and relaying light-induced conformational changes to the HK domain. In the illuminated state, the HK domain appears partially unfolded and prone to form oligomers. The oligomerization of DrBphP can be diminished by converting the molecule back to the resting Pr state by using far-red light.
authors.affiliation: Nanoscience Center, Department of Biological and Environmental Science, University of Jyväskylä , 40014 Jyväskylä, Finland.
authors.forename: Heikki
authors.initials: H
authors.lastname: Takala
authors.forename: Heli
authors.initials: H
authors.lastname: Lehtivuori
authors.forename: Henrik
authors.initials: H
authors.lastname: Hammarén
authors.forename: Vesa P
authors.initials: VP
authors.lastname: Hytönen
authors.forename: Janne A
authors.initials: JA
authors.lastname: Ihalainen
mesh_terms: D001426:Bacterial Proteins / Q000032:analysis / Q000737:chemistry / Q000378:metabolism; D034301:Deinococcus / Q000378:metabolism*; D010834:Phytochrome / Q000032:analysis / Q000737:chemistry* / Q000378:metabolism*; D011487:Protein Conformation; D017434:Protein Structure, Tertiary; D013056:Spectrophotometry, Ultraviolet / Q000379:methods
chemical_list: D001426:Bacterial Proteins; D010834:Phytochrome