title: Cell-sensitive microscopy imaging for cell image segmentation.
abstract: We propose a novel cell segmentation approach by estimating a cell-sensitive camera response function based on variously exposed phase contrast microscopy images on the same cell dish. Using the cell-sensitive microscopy imaging, cells' original irradiance signals are restored from all exposures and the irradiance signals on non-cell background regions are restored as a uniform constant (i.e., the imaging system is sensitive to cells only but insensitive to non-cell background). Cell segmentation is then performed on the restored irradiance signals by simple thresholding. The experimental results validate that high quality cell segmentation can be achieved by our approach.
authors.forename: Zhaozheng
authors.initials: Z
authors.lastname: Yin
authors.forename: Hang
authors.initials: H
authors.lastname: Su
authors.forename: Elmer
authors.initials: E
authors.lastname: Ker
authors.forename: Mingzhong
authors.initials: M
authors.lastname: Li
authors.forename: Haohan
authors.initials: H
authors.lastname: Li
mesh_terms: D000465:Algorithms; D058948:Cell Tracking / Q000379:methods*; D007089:Image Enhancement / Q000379:methods*; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D008858:Microscopy, Phase-Contrast / Q000379:methods*; D010363:Pattern Recognition, Automated / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013382:Subtraction Technique