title: The first complete genome sequences of two distinct European tomato spotted wilt virus isolates.
abstract: Tomato spotted wilt virus (TSWV) represents a major constraint to the production of important vegetable and ornamental crops in several countries around the world, including those in Europe. In spite of their economic importance, European TSWV isolates have only been partially characterized, and a complete genome sequence has not been determined yet. In this study, we completed the whole genome sequence of two distinct TSWV isolates from Italy, p105 and p202/3WT. The sequences of the L and M segments of p105 and of the L segment of p202/3WT were determined using a combined approach of RT-PCR and small RNA (sRNAs) contig assembly. Phylogenetic analysis based on RNA-dependent RNA polymerase and GN/GC protein sequences grouped the two isolates in two different clades, showing that different evolutive lineages are present among Italian TSWV isolates. Analysis of possible recombination/reassortment events among our isolates and other available full-length genome TSWV sequences showed a likely reassortment event involving the L segment.
authors.affiliation: Istituto per la Protezione Sostenibile delle Piante, Sez. di Torino, CNR, Strada delle Cacce 73, 10135, Turin, Italy.
authors.forename: P
authors.initials: P
authors.lastname: Margaria
authors.forename: L
authors.initials: L
authors.lastname: Miozzi
authors.forename: M
authors.initials: M
authors.lastname: Ciuffo
authors.forename: H R
authors.initials: HR
authors.lastname: Pappu
authors.forename: M
authors.initials: M
authors.lastname: Turina
mesh_terms: D001483:Base Sequence; D018556:Crops, Agricultural / Q000821:virology; D005060:Europe; D016679:Genome, Viral / Q000235:genetics; D018551:Solanum lycopersicum / Q000821:virology*; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology*; D012367:RNA, Viral / Q000235:genetics; D012324:RNA-Dependent RNA Polymerase / Q000235:genetics; D016865:Reassortant Viruses / Q000235:genetics; D017423:Sequence Analysis, RNA; D017865:Tospovirus / Q000235:genetics* / Q000302:isolation & purification; D014764:Viral Proteins / Q000235:genetics
chemical_list: D012367:RNA, Viral; D014764:Viral Proteins; D012324:RNA-Dependent RNA Polymerase