title: Screening rectal swabs for carbapenemase genes.
abstract: In an outbreak setting, we screened 16,296 samples from 3,644 patients by PCR for the presence of blaOXA-48, blaVIM, blaIMP, blaNDM, and blaKPC. The blaOXA-48 gene was found in samples from 43 patients infected with 9 different species of Enterobacteriaceae. Five patients had Pseudomonas aeruginosa isolates containing blaVIM. The negative predictive value of screening was 100%, and the positive predictive value was 86%.
authors.affiliation: Maasstad Laboratory, Molecular Diagnostics Unit, Rotterdam, The Netherlands zeea@maasstadziekenhuis.nl.
authors.forename: Anneke
authors.initials: A
authors.lastname: van der Zee
authors.affiliation: Maasstad Laboratory, Molecular Diagnostics Unit, Rotterdam, The Netherlands.
authors.forename: Lieuwe
authors.initials: L
authors.lastname: Roorda
authors.affiliation: Maasstad Laboratory, Molecular Diagnostics Unit, Rotterdam, The Netherlands.
authors.forename: Gerda
authors.initials: G
authors.lastname: Bosman
authors.affiliation: Maasstad Laboratory, Molecular Diagnostics Unit, Rotterdam, The Netherlands Department of Medical Microbiology and Infectious Diseases, Erasmus Medical Centre, Rotterdam, The Netherlands.
authors.forename: Jacobus M
authors.initials: JM
authors.lastname: Ossewaarde
mesh_terms: D001426:Bacterial Proteins / Q000032:analysis* / Q000235:genetics; D004196:Disease Outbreaks; D004755:Enterobacteriaceae / Q000201:enzymology* / Q000235:genetics; D016905:Gram-Negative Bacterial Infections / Q000175:diagnosis / Q000453:epidemiology / Q000382:microbiology*; D006801:Humans; D008403:Mass Screening / Q000379:methods*; D025202:Molecular Diagnostic Techniques / Q000379:methods; D016133:Polymerase Chain Reaction / Q000379:methods*; D011237:Predictive Value of Tests; D011550:Pseudomonas aeruginosa / Q000201:enzymology* / Q000235:genetics; D012007:Rectum / Q000382:microbiology*; D001618:beta-Lactamases / Q000032:analysis* / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D001618:beta-Lactamases; C063912:carbapenemase