title: Identification of the role of a MAP kinase Tmk2 in Hypocrea jecorina (Trichoderma reesei).
abstract: Despite the important role of MAPKs in signal transduction, their functions in the cellulase hyper-producing filamentous fungus Hypocrea jecorina haven't been studied except for the Hog1-like Tmk3. In this work, we constructed and explored the features of H. jecorina Δtmk2 to identify the role of this Slt2-homologous Tmk2. It is suggested from the results that Tmk2 is involved in cell wall integrity, sporulation and cellulase production. Although bearing similar roles in cell wall integrity maintenance, Tmk2 and Tmk3 appear to also have distinct functions: Tmk3 participates in high osmolarity resistance while Tmk2 does not; Tmk2 participates in sporulation but not Tmk3; Tmk3 is involved in promoting cellulase production while Tmk2 is involved in repressing cellulase formation. These studies provide the first insight into the function of Tmk2 in H. jecorina and contribute to understanding the signal transduction processes leading to the regulation of cellulase production in this important cellulase hyper-producer.
authors.affiliation: 1] State Key Laboratory of Microbial Technology, School of Life Sciences, Shandong University, Jinan 250100, China [2] National Glycoengineering Research Center, Shandong University, Jinan 250100, China.
authors.forename: Mingyu
authors.initials: M
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Microbial Technology, School of Life Sciences, Shandong University, Jinan 250100, China.
authors.forename: Yanmei
authors.initials: Y
authors.lastname: Dong
authors.affiliation: State Key Laboratory of Microbial Technology, School of Life Sciences, Shandong University, Jinan 250100, China.
authors.forename: Qiushuang
authors.initials: Q
authors.lastname: Zhao
authors.affiliation: State Key Laboratory of Microbial Technology, School of Life Sciences, Shandong University, Jinan 250100, China.
authors.forename: Fangzhong
authors.initials: F
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Microbial Technology, School of Life Sciences, Shandong University, Jinan 250100, China.
authors.forename: Kuimei
authors.initials: K
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Microbial Technology, School of Life Sciences, Shandong University, Jinan 250100, China.
authors.forename: Baojie
authors.initials: B
authors.lastname: Jiang
authors.affiliation: 1] State Key Laboratory of Microbial Technology, School of Life Sciences, Shandong University, Jinan 250100, China [2] National Glycoengineering Research Center, Shandong University, Jinan 250100, China.
authors.forename: Xu
authors.initials: X
authors.lastname: Fang
mesh_terms: D002473:Cell Wall / Q000201:enzymology / Q000378:metabolism; D002480:Cellulase / Q000096:biosynthesis*; D005285:Fermentation; D035901:Hypocrea / Q000201:enzymology*; D020929:Mitogen-Activated Protein Kinase Kinases / Q000737:chemistry / Q000378:metabolism*; D015398:Signal Transduction / Q000235:genetics; D013172:Spores, Fungal / Q000254:growth & development* / Q000378:metabolism
chemical_list: D020929:Mitogen-Activated Protein Kinase Kinases; D002480:Cellulase