title: Selenate reductase activity in Escherichia coli requires Isc iron-sulfur cluster biosynthesis genes.
abstract: The selenate reductase in Escherichia coli is a multi-subunit enzyme predicted to bind Fe-S clusters. In this study, we examined the iron-sulfur cluster biosynthesis genes that are required for selenate reductase activity. Mutants devoid of either the iscU or hscB gene in the Isc iron-sulfur cluster biosynthesis pathway lost the ability to reduce selenate. Genetic complementation by the wild-type sequences restored selenate reductase activity. The results indicate the Isc biosynthetic system plays a key role in selenate reductase Fe-S cofactor assembly and is essential for enzyme activity.
authors.affiliation: School of Environmental and Biological Sciences, Rutgers, The State University of New Jersey, New Brunswick, NJ, USA.
authors.forename: Nathan
authors.initials: N
authors.lastname: Yee
authors.forename: Jessica
authors.initials: J
authors.lastname: Choi
authors.forename: Abigail W
authors.initials: AW
authors.lastname: Porter
authors.forename: Sean
authors.initials: S
authors.lastname: Carey
authors.forename: Ines
authors.initials: I
authors.lastname: Rauschenbach
authors.forename: Arye
authors.initials: A
authors.lastname: Harel
mesh_terms: D053898:Biosynthetic Pathways; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism; D006360:Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism; D007501:Iron / Q000378:metabolism*; D007506:Iron-Sulfur Proteins / Q000235:genetics / Q000378:metabolism; D005810:Multigene Family; D010084:Oxidation-Reduction; D010088:Oxidoreductases / Q000235:genetics / Q000378:metabolism*; D064586:Selenic Acid / Q000378:metabolism; D013455:Sulfur / Q000378:metabolism*
chemical_list: D029968:Escherichia coli Proteins; D006360:Heat-Shock Proteins; C095453:HscB protein, E coli; D007506:Iron-Sulfur Proteins; C411665:IscU protein, E coli; D013455:Sulfur; D007501:Iron; D010088:Oxidoreductases; C078108:selenate reductase; D064586:Selenic Acid
keywords: Enterobacter; FNR; Salmonella; Suf; TAT; molybdoenzyme