title: AFM sensing cortical actin cytoskeleton destabilization during plasma membrane electropermeabilization.
abstract: Electropermeabilization is a physical method that uses electric field pulses to deliver molecules into cells and tissues. Despite its increasing interest in clinics, little is known about plasma membrane destabilization process occurring during electropermeabilization. Having previously demonstrated the usefulness of Atomic Force Microscopy to study electropermeabilization effect on plasma membrane, we further investigated the plasma membrane destabilization process. We got mainly interested in the cytoskeleton role in stiffness of the plasma membrane, and thus in the effect of electric field on the cortical actin network. We show here that cortical actin is destabilized by electric pulses and that this effect is not directly related to the electropermeabilization of the plasma membrane.
authors.affiliation: Centre National de la Recherche Scientifique, Laboratoire d'Analyse et d'Architecture des Systèmes (LAAS), NanoBioSystem Group, Toulouse, F-31400, France; Centre National de la Recherche Scientifique, Institut de Pharmacologie et de Biologie Structurale (IPBS), Cell Biophysics Group, UMR 5089, BP64182, F-31077, Toulouse Cedex 4, France; Université de Toulouse, UPS, INSA, INP, ISAE, UT1, UTM, LAAS, ITAV, F-31077, Toulouse Cedex 4, France.
authors.forename: Chopinet
authors.initials: C
authors.lastname: Louise
authors.forename: Dague
authors.initials: D
authors.lastname: Etienne
authors.forename: Rols
authors.initials: R
authors.lastname: Marie-Pierre
mesh_terms: D008841:Actin Cytoskeleton / Q000187:drug effects / Q000378:metabolism*; D000818:Animals; D019086:Bridged Bicyclo Compounds, Heterocyclic / Q000494:pharmacology; D016466:CHO Cells; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism*; D006224:Cricetinae; D003412:Cricetulus; D004548:Elasticity / Q000187:drug effects; D018274:Electroporation / Q000379:methods*; D018625:Microscopy, Atomic Force; D053778:Thiazolidines / Q000494:pharmacology
chemical_list: D019086:Bridged Bicyclo Compounds, Heterocyclic; D053778:Thiazolidines; C037068:latrunculin B
keywords: actin cytoskeleton; atomic force microscope; electroporation; force spectroscopy; living cells; quantitative imaging