title: Novel rolling circle amplification and DNA origami-based DNA belt-involved signal amplification assay for highly sensitive detection of prostate-specific antigen (PSA).
abstract: Prostate-specific antigen (PSA) is one of the most important biomarkers for the early diagnosis and prognosis of prostate cancer. Although many efforts have been made to achieve significant progress for the detection of PSA, challenges including relative low sensitivity, complicated operation, sophisticated instruments, and high cost remain unsolved. Here, we have developed a strategy combining rolling circle amplification (RCA)-based DNA belts and magnetic bead-based enzyme-linked immunosorbent assay (ELISA) for the highly sensitive and specific detection of PSA. At first, a 96-base circular DNA template was designed and prepared for the following RCA. Single stranded DNA (ssDNA) products from RCA were used as scaffold strand for DNA origami, which was hybridized with three staple strands of DNA. The resulting DNA belts were conjugated with multiple enzymes for signal amplification and then employed to magnetic bead based ELISA for PSA detection. Through our strategy, as low as 50 aM of PSA can be detected with excellent specificity.
authors.affiliation: Department of Medical Imaging, Jinan Military General Hospital , Shandong Province 250031, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Yan
authors.forename: Chongya
authors.initials: C
authors.lastname: Hu
authors.forename: Ping
authors.initials: P
authors.lastname: Wang
authors.forename: Rui
authors.initials: R
authors.lastname: Liu
authors.forename: Xiaolei
authors.initials: X
authors.lastname: Zuo
authors.forename: Xunwei
authors.initials: X
authors.lastname: Liu
authors.forename: Shiping
authors.initials: S
authors.lastname: Song
authors.forename: Chunhai
authors.initials: C
authors.lastname: Fan
authors.forename: Dannong
authors.initials: D
authors.lastname: He
authors.forename: Gang
authors.initials: G
authors.lastname: Sun
mesh_terms: D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D004277:DNA, Single-Stranded / Q000737:chemistry / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D008297:Male; D049329:Nanostructures / Q000737:chemistry; D021141:Nucleic Acid Amplification Techniques; D017430:Prostate-Specific Antigen / Q000032:analysis* / Q000235:genetics; D011471:Prostatic Neoplasms / Q000175:diagnosis; D012680:Sensitivity and Specificity
chemical_list: D004277:DNA, Single-Stranded; D004247:DNA; D017430:Prostate-Specific Antigen
keywords: DNA nanostructures; DNA origami; prostate-specific antigen (PSA); rolling circle amplification (RCA); signal amplification