title: Multiplex SNP detection in multiple codons for accurate drug therapy.
abstract: Multiplex SNP discrimination in the multiple codons of the genomic DNA is demonstrated by applying controller DNA technology (CDT) to MDR-TB 9G DNAChips. CDT allowed the efficient detection of 20 SNPs in five codons of the genomic DNA in 40 min. CDT could distinguish SNP targets to as low as 1 copy of the genomic DNA. 100% agreement with the sequencing analysis of clinical samples highlighted the clinical applicability of MDR-TB 9G DNAChips.
authors.affiliation: Institute for Applied Chemistry and Department of Chemistry, Hallym University, Chuncheon, 200-702, Korea. tskim@hallym.ac.kr.
authors.forename: Danishmalik Rafiq
authors.initials: DR
authors.lastname: Sayyed
authors.forename: Satish Balasaheb
authors.initials: SB
authors.lastname: Nimse
authors.forename: Keum-Soo
authors.initials: KS
authors.lastname: Song
authors.forename: Nackmoon
authors.initials: N
authors.lastname: Sung
authors.forename: Taisun
authors.initials: T
authors.lastname: Kim
mesh_terms: D003062:Codon / Q000235:genetics*; D004247:DNA / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis; D004364:Pharmaceutical Preparations; D010597:Pharmacogenetics / Q000379:methods*; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D017422:Sequence Analysis, DNA / Q000379:methods*
chemical_list: D003062:Codon; D004364:Pharmaceutical Preparations; D004247:DNA