title: One strain-many compounds (OSMAC) method for production of polyketides, azaphilones, and an isochromanone using the endophytic fungus Dothideomycete sp.
abstract: Polyketides 1-6 were produced by a one strain-many compounds (OSMAC) approach using the endophytic fungus Dothideomycete sp. CRI7 as a producer. Metabolite production of the fungus Dothideomycete sp. CRI7 was sensitive to sources of potato and malt extract used for the preparation of PDB and Czapek malt media, respectively. Three hitherto unknown metabolites were obtained from the fungus CRI7 grown in PDB medium prepared from a commercial potato powder instead of fresh tubers of potato, while three others were obtained from the fungus cultivated in Czapek malt medium. Moreover, a source of malt extract used in the Czapek malt medium was found to influence metabolite production by the fungus CRI7. Structure elucidation of these compounds was achieved by analysis of spectroscopic data, as well as by single crystal X-ray analysis. Two of the compounds showed weak cytotoxic activity, while the remainders were inactive toward the cell lines tested. One compound exhibited radical scavenging activity with an IC50 value of 21.7 μM, and inhibited aromatase with an IC50 value of 12.3 μM.
authors.affiliation: Chulabhorn Graduate Institute, Chemical Biology Program, Kamphaeng Phet 6 Road, Laksi, Bangkok 10210, Thailand.
authors.forename: Ranuka T
authors.initials: RT
authors.lastname: Hewage
authors.affiliation: Department of Chemistry, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand.
authors.forename: Thammarat
authors.initials: T
authors.lastname: Aree
authors.affiliation: Chulabhorn Graduate Institute, Chemical Biology Program, Kamphaeng Phet 6 Road, Laksi, Bangkok 10210, Thailand; Chulabhorn Research Institute, Kamphaeng Phet 6 Road, Laksi, Bangkok 10210, Thailand.
authors.forename: Chulabhorn
authors.initials: C
authors.lastname: Mahidol
authors.affiliation: Chulabhorn Graduate Institute, Chemical Biology Program, Kamphaeng Phet 6 Road, Laksi, Bangkok 10210, Thailand; Chulabhorn Research Institute, Kamphaeng Phet 6 Road, Laksi, Bangkok 10210, Thailand; Center of Excellence on Environmental Health and Toxicology (EHT), CHE, Ministry of Education, Bangkok, Thailand.
authors.forename: Somsak
authors.initials: S
authors.lastname: Ruchirawat
authors.affiliation: Chulabhorn Graduate Institute, Chemical Biology Program, Kamphaeng Phet 6 Road, Laksi, Bangkok 10210, Thailand; Chulabhorn Research Institute, Kamphaeng Phet 6 Road, Laksi, Bangkok 10210, Thailand; Center of Excellence on Environmental Health and Toxicology (EHT), CHE, Ministry of Education, Bangkok, Thailand. Electronic address: prasat@cri.or.th.
authors.forename: Prasat
authors.initials: P
authors.lastname: Kittakoop
mesh_terms: D001203:Ascomycota / Q000737:chemistry*; D001578:Benzopyrans / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D002867:Chromones / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D018360:Crystallography, X-Ray; D006801:Humans; D008968:Molecular Conformation; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D010860:Pigments, Biological / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D061065:Polyketides / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D013785:Thailand
chemical_list: D001578:Benzopyrans; D002867:Chromones; D010860:Pigments, Biological; D061065:Polyketides; C494154:azaphilone
keywords: Azaphilone; Cytotoxic activity; Dothideomycete sp.; Endophytic fungi; Fungal metabolites; Isochromanone; OSMAC; One strain-many compounds; Polyketide