title: Characterization of Alkaliphilus hydrothermalis sp. nov., a novel alkaliphilic anaerobic bacterium, isolated from a carbonaceous chimney of the Prony hydrothermal field, New Caledonia.
abstract: A novel anaerobic, alkaliphilic, Gram-positive staining bacterium was isolated from a hydrothermal chimney in the Prony Bay, New Caledonia. This strain designated FatMR1(T) grew at temperatures from 20 to 55 °C (optimum 37 °C) and at pH between 7.5 and 10.5 (optimum 8.8-9). NaCl is not required for growth (optimum 0.2-0.5%), but is tolerated up to 3%. Sulfate, thiosulfate, elemental sulfur, sulfite, nitrate and nitrite are not used as terminal electron acceptors. Strain FatMR1(T) fermented pyruvate, yeast extract, peptone and biotrypcase and used fructose as the only sugar. The main fermentation products from fructose and proteinaceous compounds (e.g. peptone and biotrypcase) were acetate, H2 and CO2. Crotonate was disproportionated to acetate and butyrate. The predominant cellular fatty acids were C14:0 and C16:0. The G + C content of the genomic DNA was 37.1 mol%. On the basis of phylogenetic, genetic, and physiological properties, strain FatMR1(T) (=DSM 25890(T), =JCM 18390(T)) belonging to the phylum Firmicutes, class Clostridia, order Clostridiales, is proposed as a novel species of the genus Alkaliphilus, A. hydrothermalis sp. nov.
authors.affiliation: IRD, Mediterranean Institute of Oceanography (MIO), CNRS/INSU, Aix-Marseille Université, Université du Sud Toulon-Var, UM 110, 13288, Marseille Cedex 09, France.
authors.forename: Fatma
authors.initials: F
authors.lastname: Ben Aissa
authors.forename: Anne
authors.initials: A
authors.lastname: Postec
authors.forename: Gaël
authors.initials: G
authors.lastname: Erauso
authors.forename: Claude
authors.initials: C
authors.lastname: Payri
authors.forename: Bernard
authors.initials: B
authors.lastname: Pelletier
authors.forename: Moktar
authors.initials: M
authors.lastname: Hamdi
authors.forename: Marie-Laure
authors.initials: ML
authors.lastname: Fardeau
authors.forename: Bernard
authors.initials: B
authors.lastname: Ollivier
mesh_terms: D000693:Anaerobiosis; D001482:Base Composition; D002244:Carbon / Q000737:chemistry*; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D005285:Fermentation; D005632:Fructose / Q000737:chemistry; D006094:Gram-Positive Bacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D006863:Hydrogen-Ion Concentration; D060086:Hydrothermal Vents / Q000382:microbiology*; D009510:New Caledonia; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D013696:Temperature
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D005632:Fructose; D002244:Carbon