title: Modulation of (-)-epicatechin metabolism by coadministration with other polyphenols in Caco-2 cell model.
abstract: Widely consumed beverages such as red wine, tea, and cocoa-derived products are a great source of flavanols. Epidemiologic and interventional studies suggest that cocoa flavanols such as (-)-epicatechin may reduce the risk of cardiovascular diseases. The interaction of (-)-epicatechin with food components including other polyphenols could modify its absorption, metabolism, and finally its bioactivity. In the present study we investigate (-)-epicatechin absorption and metabolism when coexposed with other polyphenols in the intestinal absorptive Caco-2 cell model. Depending on the type of polyphenols coadministered, the total amount of 3'-O-methyl-epicatechin and 3'-O-sulfate-epicatechin conjugates found both in apical and basal compartments ranged from 19 to 801 nM and from 6 to 432 nM, respectively. The coincubation of (-)-epicatechin with flavanols, chlorogenic acid, and umbelliferone resulted in similar amounts of 3'-O-methyl-epicatechin effluxed into the apical compartment relative to control. Coincubation with isorhamnetin, kaempferol, diosmetin, nevadensin, chrysin, equol, genistein, and hesperitin promoted the transport of 3'-O-methyl-epicatechin toward the basolateral side and decreased the apical efflux. Quercetin and luteolin considerably inhibited the appearance of this (-)-epicatechin conjugate both in the apical and basolateral compartments. In conclusion, we could demonstrate that the efflux of (-)-epicatechin conjugates to the apical or basal compartments of Caco-2 cells is modulated by certain classes of polyphenols and their amount. Ingesting (-)-epicatechin with specific polyphenols could be a strategy to increase the bioavailability of (-)-epicatechin and to modulate its metabolic profile.
authors.affiliation: Nestlé Research Center, Nestec Ltd., Lausanne, Switzerland (B.S.B., A.L., E.B., A.P., L.A.G.); Division of Toxicology, Wageningen University, Tuinlaan, Wageningen, The Netherlands (H.L.).
authors.forename: Belén
authors.initials: B
authors.lastname: Sanchez-Bridge
authors.affiliation: Nestlé Research Center, Nestec Ltd., Lausanne, Switzerland (B.S.B., A.L., E.B., A.P., L.A.G.); Division of Toxicology, Wageningen University, Tuinlaan, Wageningen, The Netherlands (H.L.).
authors.forename: Antoine
authors.initials: A
authors.lastname: Lévèques
authors.affiliation: Nestlé Research Center, Nestec Ltd., Lausanne, Switzerland (B.S.B., A.L., E.B., A.P., L.A.G.); Division of Toxicology, Wageningen University, Tuinlaan, Wageningen, The Netherlands (H.L.).
authors.forename: Hequn
authors.initials: H
authors.lastname: Li
authors.affiliation: Nestlé Research Center, Nestec Ltd., Lausanne, Switzerland (B.S.B., A.L., E.B., A.P., L.A.G.); Division of Toxicology, Wageningen University, Tuinlaan, Wageningen, The Netherlands (H.L.).
authors.forename: Emmanuelle
authors.initials: E
authors.lastname: Bertschy
authors.affiliation: Nestlé Research Center, Nestec Ltd., Lausanne, Switzerland (B.S.B., A.L., E.B., A.P., L.A.G.); Division of Toxicology, Wageningen University, Tuinlaan, Wageningen, The Netherlands (H.L.).
authors.forename: Amaury
authors.initials: A
authors.lastname: Patin
authors.affiliation: Nestlé Research Center, Nestec Ltd., Lausanne, Switzerland (B.S.B., A.L., E.B., A.P., L.A.G.); Division of Toxicology, Wageningen University, Tuinlaan, Wageningen, The Netherlands (H.L.) lucas.actisgoretta@rdls.nestle.com.
authors.forename: Lucas
authors.initials: L
authors.lastname: Actis-Goretta
mesh_terms: D001692:Biological Transport / Q000502:physiology; D018938:Caco-2 Cells; D002392:Catechin / Q000378:metabolism*; D045744:Cell Line, Tumor; D006801:Humans; D007408:Intestinal Absorption / Q000502:physiology; D007413:Intestinal Mucosa / Q000378:metabolism; D059808:Polyphenols / Q000378:metabolism*
chemical_list: D059808:Polyphenols; D002392:Catechin