title: Impact of RTN3 deficiency on expression of BACE1 and amyloid deposition.
abstract: Reticulon 3 (RTN3) has previously been shown to interact with BACE1 and negatively regulate BACE1 activity. To what extent RTN3 deficiency affects BACE1 activity is an intriguing question. In this study, we aimed to address this by generating RTN3-null mice. Mice with complete deficiency of RTN3 grow normally and have no obviously discernible phenotypes. Morphological analyses of RTN3-null mice showed no significant alterations in cellular structure, although RTN3 is recognized as a protein contributing to the shaping of tubular endoplasmic reticulum. Biochemical analysis revealed that RTN3 deficiency increased protein levels of BACE1. This elevation of BACE1 levels correlated with enhanced processing of amyloid precursor protein at the β-secretase site. We also demonstrated that RTN3 deficiency in Alzheimer's mouse models facilitates amyloid deposition, further supporting an in vivo role of RTN3 in the regulation of BACE1 activity. Since it has been shown that RTN3 monomer is reduced in brains of Alzheimer's patients, our results suggest that long-lasting reduction of RTN3 levels has adverse effects on BACE1 activity and may contribute to Alzheimer's pathogenesis.
authors.affiliation: Department of Neurosciences, Lerner Research Institute, Cleveland Clinic Foundation, Cleveland, Ohio 44195 and.
authors.forename: Qi
authors.initials: Q
authors.lastname: Shi
authors.affiliation: Department of Neurosciences, Lerner Research Institute, Cleveland Clinic Foundation, Cleveland, Ohio 44195 and.
authors.forename: Yingying
authors.initials: Y
authors.lastname: Ge
authors.affiliation: Department of Neurosciences, Lerner Research Institute, Cleveland Clinic Foundation, Cleveland, Ohio 44195 and.
authors.forename: Md Golam
authors.identifier: http://orcid.org/0000-0001-7581-6977
authors.initials: MG
authors.lastname: Sharoar
authors.affiliation: Department of Neurosciences, Lerner Research Institute, Cleveland Clinic Foundation, Cleveland, Ohio 44195 and.
authors.forename: Wanxia
authors.initials: W
authors.lastname: He
authors.affiliation: Department of Cell Biology, School of Life Sciences, State Key Laboratory of Medical Genetics, Central South University, 410083 Changsha, People's Republic of China.
authors.forename: Rong
authors.initials: R
authors.lastname: Xiang
authors.affiliation: Department of Cell Biology, School of Life Sciences, State Key Laboratory of Medical Genetics, Central South University, 410083 Changsha, People's Republic of China.
authors.forename: Zhuohua
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Department of Neurosciences, Lerner Research Institute, Cleveland Clinic Foundation, Cleveland, Ohio 44195 and.
authors.forename: Xiangyou
authors.initials: X
authors.lastname: Hu
authors.affiliation: Department of Neurosciences, Lerner Research Institute, Cleveland Clinic Foundation, Cleveland, Ohio 44195 and yanr@ccf.org.
authors.forename: Riqiang
authors.initials: R
authors.lastname: Yan
mesh_terms: D000544:Alzheimer Disease / Q000378:metabolism / Q000473:pathology; D053829:Amyloid Precursor Protein Secretases / Q000096:biosynthesis*; D000818:Animals; D016282:Aspartic Acid Endopeptidases / Q000096:biosynthesis*; D001921:Brain / Q000378:metabolism / Q000473:pathology; D005260:Female; D015971:Gene Expression Regulation, Enzymologic; D051379:Mice; D057507:Mice, 129 Strain; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008822:Mice, Transgenic; D009419:Nerve Tissue Proteins / Q000172:deficiency*; D058225:Plaque, Amyloid / Q000378:metabolism* / Q000473:pathology
chemical_list: D009419:Nerve Tissue Proteins; C482720:Rtn3 protein, mouse; D053829:Amyloid Precursor Protein Secretases; D016282:Aspartic Acid Endopeptidases; C509282:Bace1 protein, mouse
keywords: APP; Alzheimer's disease; BACE1; Nogo; neuritic plaques; reticulon