title: Ethanol production from glycerol using immobilized Pachysolen tannophilus during microaerated repeated-batch fermentor culture.
abstract: Herein, we established a repeated-batch process for ethanol production from glycerol by immobilized Pachysolen tannophilus. The aim of this study was to develop a more practical and applicable ethanol production process for biofuel. In particular, using industrial-grade medium ingredients, the microaeration rate was optimized for maximization of the ethanol production, and the relevant metabolic parameters were then analyzed. The microaeration rate of 0.11 vvm, which is far lower than those occurring in a shaking flask culture, was found to be the optimal value for ethanol production from glycerol. In addition, it was found that, among those tested, Celite was a more appropriate carrier for the immobilization of P. tannophilus to induce production of ethanol from glycerol. Finally, through a repeated-batch culture, the ethanol yield (Ye/g) of 0.126 ± 0.017 g-ethanol/g-glycerol (n = 4) was obtained, and this value was remarkably comparable with a previous report. In the future, it is expected that the results of this study will be applied for the development of a more practical and profitable long-term ethanol production process, thanks to the industrial-grade medium preparation, simple immobilization method, and easy repeated-batch operation.
authors.affiliation: Department of Biotechnology, Korea National University of Transportation, Jeungpyung 368-701, Republic of Korea.
authors.forename: Hye-Geun
authors.initials: HG
authors.lastname: Cha
authors.forename: Yi-Ok
authors.initials: YO
authors.lastname: Kim
authors.forename: Woon Yong
authors.initials: WY
authors.lastname: Choi
authors.forename: Do-Hyung
authors.initials: DH
authors.lastname: Kang
authors.forename: Hyeon-Yong
authors.initials: HY
authors.lastname: Lee
authors.forename: Kyung-Hwan
authors.initials: KH
authors.lastname: Jung
mesh_terms: D061249:Batch Cell Culture Techniques; D056804:Biofuels; D018914:Cells, Immobilized / Q000378:metabolism; D007692:Diatomaceous Earth; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation; D005990:Glycerol / Q000378:metabolism*; D004718:Saccharomycetales / Q000254:growth & development / Q000378:metabolism* / Q000648:ultrastructure
chemical_list: D056804:Biofuels; D000431:Ethanol; D007692:Diatomaceous Earth; D005990:Glycerol