title: Zebrafish as a model to study live mucus physiology.
abstract: Dysfunctional mucus barriers can result in important pulmonary and gastrointestinal conditions, but model systems to study the underlying causes are largely missing. We identified and characterized five mucin homologues in zebrafish, and demonstrated a strategy for fluorescence labeling of one selected mucin. These tools can be used for in vivo experiments and in pharmacological and genetic screens to study the dynamics and mechanisms of mucosal physiology.
authors.affiliation: Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA.
authors.forename: Irena
authors.initials: I
authors.lastname: Jevtov
authors.affiliation: Department of Medical Biochemistry and Cell Biology, Institute of Biomedicine, Sahlgrenska Academy at University of Gothenburg, Göteborg, Sweden.
authors.forename: Tore
authors.initials: T
authors.lastname: Samuelsson
authors.affiliation: Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA.
authors.forename: Grace
authors.initials: G
authors.lastname: Yao
authors.affiliation: David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA.
authors.forename: Adam
authors.initials: A
authors.lastname: Amsterdam
authors.affiliation: Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA.
authors.forename: Katharina
authors.initials: K
authors.lastname: Ribbeck
mesh_terms: D000818:Animals; D041981:Gastrointestinal Tract / Q000502:physiology; D008954:Models, Biological; D009077:Mucins / Q000235:genetics* / Q000378:metabolism; D009093:Mucus / Q000502:physiology*; D012137:Respiratory System / Q000503:physiopathology; D014566:Urogenital System / Q000502:physiology; D015027:Zebrafish / Q000235:genetics* / Q000502:physiology
chemical_list: D009077:Mucins