title: Doxorubicin encapsulated in micelles enhances radiosensitivity in doxorubicin-resistant tumor cells.
abstract: To evaluate the efficacy of doxorubicin (DOX) loaded micelles in enhancing DOX radiosensitivity in DOX-resistant K562 tumor cells (K562/DOX cells), DOX loaded polyethylene glycol-polycaprolactone (PEG-PCL) copolymer micelles and pluronic 105 (P105) micelles, and composite micelles composed of PEG-PCL and P105 were prepared. By using MTT assay, soft agar cloning assays, confocal laser scanning microscopy, and flow cytometry analyses to evaluate the radiosensitivity of each compound, DOX loaded micelles were found to increase the radiosensitivity of K562/DOX cells, as revealed by a marked cellular uptake and its sustained, slower release than free DOX. The micelles encapsulating DOX significantly enhanced its cytotoxicity in K562/DOX cells. Combined treatment with the encapsulation of DOX in micelles and radiotherapy therefore warrants investigation in clinical trials as a potential anticancer strategy with increased efficacy and reduced side effects.
authors.affiliation: Department of Radiation Oncology, The Second Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang, 310009, China.
authors.forename: Wenhong
authors.initials: W
authors.lastname: Xu
authors.affiliation: Institute of Pharmaceutics, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou, Zhejiang, 310009, China.
authors.forename: Min
authors.initials: M
authors.lastname: Han
authors.affiliation: Institute of Pharmaceutics, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou, Zhejiang, 310009, China.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Diao
authors.affiliation: Cancer Institute (Key Laboratory, China National Ministry of Education), The Second Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang, 310009, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Pan
authors.affiliation: Cancer Institute (Key Laboratory, China National Ministry of Education), The Second Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang, 310009, China.
authors.forename: Weiting
authors.initials: W
authors.lastname: Ge
authors.affiliation: Cancer Institute (Key Laboratory, China National Ministry of Education), The Second Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang, 310009, China.
authors.forename: Hai
authors.initials: H
authors.lastname: Liu
authors.affiliation: Cancer Institute (Key Laboratory, China National Ministry of Education), The Second Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang, 310009, China.
authors.forename: Hong
authors.initials: H
authors.lastname: He
authors.affiliation: Department of Polymer Science and Engineering, Zhejiang University, Hangzhou, Zhejiang, 310058, China.
authors.forename: Hongliang
authors.initials: H
authors.lastname: Jiang
authors.affiliation: Cancer Institute (Key Laboratory, China National Ministry of Education), The Second Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang, 310009, China.
authors.forename: Shu
authors.initials: S
authors.lastname: Zheng
authors.affiliation: Cancer Institute (Key Laboratory, China National Ministry of Education), The Second Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang, 310009, China.
authors.forename: Suzhan
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Institute of Pharmaceutics, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou, Zhejiang, 310009, China.
authors.forename: Jianqing
authors.initials: J
authors.lastname: Gao
authors.affiliation: Department of Radiation Oncology, The Second Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang, 310009, China.
authors.forename: Qichun
authors.initials: Q
authors.lastname: Wei
mesh_terms: D000903:Antibiotics, Antineoplastic / Q000737:chemistry / Q000494:pharmacology; D004317:Doxorubicin / Q000737:chemistry / Q000494:pharmacology; D004337:Drug Carriers / Q000737:chemistry / Q000494:pharmacology; D019008:Drug Resistance, Neoplasm / Q000187:drug effects*; D006801:Humans; D020014:K562 Cells; D008823:Micelles; D011091:Polyesters / Q000737:chemistry / Q000494:pharmacology; D011092:Polyethylene Glycols / Q000737:chemistry / Q000494:pharmacology; D011836:Radiation Tolerance / Q000187:drug effects*
chemical_list: D000903:Antibiotics, Antineoplastic; D004337:Drug Carriers; D008823:Micelles; D011091:Polyesters; C016240:polycaprolactone; D011092:Polyethylene Glycols; D004317:Doxorubicin