title: Multispacer Typing (MST) of Spotted Fever Group Rickettsiae Isolated from Humans and Rats in Chengmai County, Hainan Province, China.
abstract: Spotted fever caused by spotted fever group rickettsiae (SFGR) is found throughout China. During 2007-2008, 28 human SFGR isolates and 34 rat SFGR isolates including 15 isolates from Rattus fulvescens, 5 isolates from R. edwardsi, 7 isolates from Callosciurus erythraeus roberti and 7 isolates from Dremomys rufigenis) were obtained from L929 cell culture. Previous research indicated that the 62 strains of SFGR mentioned above shared not only the same serophenotype but also 100% of identity sequences of 16S rRNA, gltA, ompA, groEL and 17KD, which enabled us to apply multispacer typing (MST) to the 62 SFGR isolates in the study. Six primer pairs, which were used for typing of Rickettsia rickettsii and Rickettsia conorii, were chosen, and the results exhibited greater nucleotide polymorphisms among the 62 isolates tested. A total of 48 distinct genotypes were identified. The dominant genotype, represented by h3 isolates, accounted for 21.7% (13/60) of the isolates tested, and the remaining 47 genotypes were all unique. Phylogenetic analysis showed that all the 48 genotypes could be classified in the same clade, while the genetically related strain, R. heilongjiangensis, was close but not the same as the cluster. We concluded that the genetically diverse of spotted fever group rickettsiae strains are endemic in Chengmai County, Hainan Province, China.
authors.affiliation: Department of Rickettsiology, National Institute for Communicable Disease Control and Prevention , China CDC, Changping, Beijing102206, People's Republic of China.
authors.forename: Xueqin
authors.initials: X
authors.lastname: Cheng
authors.affiliation: Department of Epidemiology, Hainan Provincial Center for Disease Control and Prevention , Haikou, Hainan Province, China.
authors.forename: Yuming
authors.initials: Y
authors.lastname: Jin
authors.affiliation: Department of Epidemiology, Hainan Provincial Center for Disease Control and Prevention , Haikou, Hainan Province, China.
authors.forename: Shijun
authors.initials: S
authors.lastname: Lao
authors.affiliation: Department of Epidemiology, Hainan Provincial Center for Disease Control and Prevention , Haikou, Hainan Province, China.
authors.forename: Changhe
authors.initials: C
authors.lastname: Huang
authors.affiliation: Department of Epidemiology, Hainan Provincial Center for Disease Control and Prevention , Haikou, Hainan Province, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Huang
authors.affiliation: Department of Epidemiology, Hainan Provincial Center for Disease Control and Prevention , Haikou, Hainan Province, China.
authors.forename: Pengben
authors.initials: P
authors.lastname: Jia
authors.affiliation: Department of Rickettsiology, National Institute for Communicable Disease Control and Prevention , China CDC, Changping, Beijing102206, People's Republic of China.
authors.forename: Lijuan
authors.initials: L
authors.lastname: Zhang
keywords: Chengmai County of Hainan Province; China; Chinese isolates; MST; Spotted fever group rickettsiae (SFGR); spotted fever