title: [Prophylactic effect of budesonide on the expression of IL-4, IL-5 in model of allergic rhinitis rats].
abstract: OBJECTIVE
  To explore the prophylactic effect of Budesonide on the expression of IL-4,IL-5 in nasal mucosa in model of minimal persistent inflammation of allergic rhinitis in rats.
  
  
  METHODS
  Eighty SD rats were randomly divided into allergic rhinitis group (A group), experimental (B group), control group (C group) and negative control group (D group). A group was made for model of allergic rhinitis. B and C group were made for model of the lightest persistent inflammatory response. After the models were established, half of rats in the A group, B group, C group and D group were executed, and EOS infiltration and the expression of IL-4, IL-5, ICAM-1 were observed in nasal mucosa. The remaining rats of B group were given budesonide (64 microg/side/time, twice/day) treatment for 2 weeks. A, C, D group were given nasal spray with normal saline for 2 weeks. After that A, B, and C groups were stimulated with 1% OVA daily for one week, D group were given nasal spray with normal saline. All rats were executed after excitation, EOS infiltration and IL-4, IL-5 expression were observed.
  
  
  RESULTS
  After the drug treatment, B group only had a small amount of mucous EOS infiltration and had no significant difference with D group, but in A and C group EOS had heavy infiltration. Gray value of the IL-4 positive areas in B group were significantly different compared with A and C group (P < 0.05), A group and C group had no significant difference (P > 0.05). Distribution of IL-5 positive signals was similar with that of IL-4.
  
  
  CONCLUSIONS
  Budesonide MPI application could significantly inhibit the allergic.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Guo
authors.forename: Manying
authors.initials: M
authors.lastname: Geng
authors.forename: Zhong
authors.initials: Z
authors.lastname: Li
authors.forename: Hui
authors.initials: H
authors.lastname: Zhang
authors.forename: Xiufen
authors.initials: X
authors.lastname: Wei
mesh_terms: D000818:Animals; D019819:Budesonide / Q000627:therapeutic use*; D004195:Disease Models, Animal; D005260:Female; D015847:Interleukin-4 / Q000378:metabolism*; D015848:Interleukin-5 / Q000378:metabolism*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D065631:Rhinitis, Allergic / Q000378:metabolism / Q000517:prevention & control*
chemical_list: D015848:Interleukin-5; D015847:Interleukin-4; D019819:Budesonide