title: Early gene expression in salivary gland after isoproterenol treatment.
abstract: Isoproterenol, a β-adrenergic agonist, has been shown to induce salivary gland hyperplasia. However, the mechanism involved in this pharmacological phenomenon is not well understood. To gain a better understanding of the underlying changes, including genes, networks and pathways altered by isoproterenol, microarray-based gene expression analysis was conducted on rat parotid glands at 10, 30, and 60 min after isoproterenol injection. After isoproterenol treatment, the number of differentially expressed genes was increased in a time-dependent manner. Pathway analysis showed that cell hyperplasia, p38(MAPK), and IGF-1 were the most altered function, network and pathway, respectively. The balanced regulation of up- and down-expression of genes related to cell proliferation/survival may provide a better understanding of the mechanism of isoproterenol-induced parotid gland enlargement without tumor transformation.
authors.affiliation: Department of Comprehensive Dentistry, University of Texas Health Science Center at San Antonio, San Antonio, 78229, Texas; Affiliated Hospital of Stomatology, Medical College, Zhejiang University, Hangzhou, 310000, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhou
authors.forename: Hung-I H
authors.initials: HI
authors.lastname: Chen
authors.forename: A L
authors.initials: AL
authors.lastname: Lin
authors.forename: H
authors.initials: H
authors.lastname: Dang
authors.forename: Karin
authors.initials: K
authors.lastname: Haack
authors.forename: Shelley A
authors.initials: SA
authors.lastname: Cole
authors.forename: Yufei
authors.initials: Y
authors.lastname: Huang
authors.forename: Haiyang
authors.initials: H
authors.lastname: Yu
authors.forename: Yidong
authors.initials: Y
authors.lastname: Chen
authors.forename: Chih-Ko
authors.initials: CK
authors.lastname: Yeh
mesh_terms: D000818:Animals; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000187:drug effects*; D007545:Isoproterenol / Q000494:pharmacology*; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis; D017207:Rats, Sprague-Dawley; D060888:Real-Time Polymerase Chain Reaction; D012469:Salivary Glands / Q000187:drug effects / Q000378:metabolism*
chemical_list: D007545:Isoproterenol
keywords: MAPK pathway; gene ontology and pathway analysis; parotid gland enlargement; β-adrenergic receptor