title: Molecular and morphological analysis reveals five new species of Zygophiala associated with flyspeck signs on plant hosts from China.
abstract: Species in the genus Zygophiala are associated with sooty blotch and flyspeck disease on a wide range of hosts. In this study, 63 Zygophiala isolates collected from flyspeck colonies on a range of plants from several regions of China were used for phylogeny, host range and geographic distribution analysis. Phylogenetic trees were constructed on four genes--internal transcribed spacer (ITS), partial translation elongation factor 1-alpha (TEF), β-tubulin (TUB2), and actin (ACT)--both individually and in combination. Isolates were grouped into 11 clades among which five new species, Z. emperorae, Z. trispora, Z. musae, Z. inaequalis and Z. longispora, were described. Species of Zygophiala differed in observed host range and geographic distribution. Z. wisconsinensis and Z. emperorae were the most prevalent throughout the sampled regions of China, whereas Z. trispora, Z. musae, Z. inaequalis and Z. longispora were collected only in southern China. The hosts of Z. wisconsinensis and Z. emperorae were mainly in the family Rosaceae whereas Z. trispora, Z. musae, Z. inaequalis and Z. longispora were found mainly on banana (Musa spp.). Cross inoculation tests provided evidence of host specificity among SBFS species.
authors.affiliation: Department of State Key Laboratory of Crop Stress Biology in Arid Areas and College of Plant Protection, Northwest A&F University, Yangling, Shaanxi Province, China.
authors.forename: Liu
authors.initials: L
authors.lastname: Gao
authors.affiliation: Department of State Key Laboratory of Crop Stress Biology in Arid Areas and College of Plant Protection, Northwest A&F University, Yangling, Shaanxi Province, China.
authors.forename: Mian
authors.initials: M
authors.lastname: Zhang
authors.affiliation: Department of State Key Laboratory of Crop Stress Biology in Arid Areas and College of Plant Protection, Northwest A&F University, Yangling, Shaanxi Province, China.
authors.forename: Wanyu
authors.initials: W
authors.lastname: Zhao
authors.affiliation: Department of State Key Laboratory of Crop Stress Biology in Arid Areas and College of Plant Protection, Northwest A&F University, Yangling, Shaanxi Province, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Hao
authors.affiliation: Department of State Key Laboratory of Crop Stress Biology in Arid Areas and College of Plant Protection, Northwest A&F University, Yangling, Shaanxi Province, China.
authors.forename: Hongcai
authors.initials: H
authors.lastname: Chen
authors.affiliation: Department of State Key Laboratory of Crop Stress Biology in Arid Areas and College of Plant Protection, Northwest A&F University, Yangling, Shaanxi Province, China.
authors.forename: Rong
authors.initials: R
authors.lastname: Zhang
authors.affiliation: Department of Plant Pathology and Microbiology, Iowa State University, Ames, Iowa State, United States of America.
authors.forename: Jean C
authors.initials: JC
authors.lastname: Batzer
authors.affiliation: Department of Plant Pathology and Microbiology, Iowa State University, Ames, Iowa State, United States of America.
authors.forename: Mark L
authors.initials: ML
authors.lastname: Gleason
authors.affiliation: Department of State Key Laboratory of Crop Stress Biology in Arid Areas and College of Plant Protection, Northwest A&F University, Yangling, Shaanxi Province, China.
authors.forename: Guangyu
authors.initials: G
authors.lastname: Sun
mesh_terms: D001203:Ascomycota / Q000145:classification* / Q000235:genetics*; D002681:China; D005656:Fungal Proteins / Q000235:genetics*; D010802:Phylogeny; D010935:Plant Diseases / Q000382:microbiology
chemical_list: D005656:Fungal Proteins