title: Lipidomic assessment of plasma and placenta of women with early-onset preeclampsia.
abstract: BACKGROUND
  Adipose tissue is responsible for triggering chronic systemic inflammatory response and these changes may be involved in the pathophysiology of preeclampsia.
  
  
  OBJECTIVE
  To characterize the lipid profile in the placenta and plasma of patients with preeclampsia.
  
  
  METHODS
  Samples were collected from placenta and plasma of 10 pregnant women with preeclampsia and 10 controls. Lipids were extracted using the Bligh-Dyer protocol and were analysed by MALDI TOF-TOF mass spectrometry.
  
  
  RESULTS
  Approximately 200 lipid signals were quantified. The most prevalent lipid present in plasma of patients with preeclampsia was the main class Glycerophosphoserines-GP03 (PS) representing 52.30% of the total lipid composition, followed by the main classes Glycerophosphoethanolamines-GP02 (PEt), Glycerophosphocholines-GP01 (PC) and Flavanoids-PK12 (FLV), with 24.03%, 9.47% and 8.39% respectively. When compared to the control group, plasma samples of patients with preeclampsia showed an increase of PS (p<0.0001), PC (p<0.0001) and FLV (p<0.0001). Placental analysis of patients with preeclampsia, revealed the PS as the most prevalent lipid representing 56.28%, followed by the main class Macrolides/polyketides-PK04 with 32.77%, both with increased levels when compared with patients control group, PS (p<0.0001) and PK04 (p<0.0001).
  
  
  CONCLUSIONS
  Lipids found in placenta and plasma from patients with preeclampsia differ from those of pregnant women in the control group. Further studies are needed to clarify if these changes are specific and a cause or consequence of preeclampsia.
authors.affiliation: Department of Obstetrics - Federal University of Sao Paulo, Sao Paulo, Sao Paulo, Brazil; Laboratory of Clinical and Experimental Investigation - School Maternity Vila Nova Cachoeirinha, Sao Paulo, Sao Paulo, Brazil.
authors.forename: Henri Augusto
authors.initials: HA
authors.lastname: Korkes
authors.affiliation: Department of Obstetrics - Federal University of Sao Paulo, Sao Paulo, Sao Paulo, Brazil; Laboratory of Clinical and Experimental Investigation - School Maternity Vila Nova Cachoeirinha, Sao Paulo, Sao Paulo, Brazil.
authors.forename: Nelson
authors.initials: N
authors.lastname: Sass
authors.affiliation: Department of Obstetrics - Federal University of Sao Paulo, Sao Paulo, Sao Paulo, Brazil.
authors.forename: Antonio F
authors.initials: AF
authors.lastname: Moron
authors.affiliation: Department of Immunology - University of Sao Paulo, Sao Paulo, Sao Paulo, Brazil.
authors.forename: Niels Olsen S
authors.initials: NO
authors.lastname: Câmara
authors.affiliation: Department of Gynecology - Federal University of Sao Paulo, Sao Paulo, Sao Paulo, Brazil.
authors.forename: Tatiana
authors.initials: T
authors.lastname: Bonetti
authors.affiliation: Department of Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts, United States of America.
authors.forename: Ana Sofia
authors.initials: AS
authors.lastname: Cerdeira
authors.affiliation: Department of Gynecology - Federal University of Sao Paulo, Sao Paulo, Sao Paulo, Brazil.
authors.forename: Ismael Dale Cotrim Guerreiro
authors.initials: ID
authors.lastname: Da Silva
authors.affiliation: Department of Obstetrics - Federal University of Sao Paulo, Sao Paulo, Sao Paulo, Brazil; Laboratory of Clinical and Experimental Investigation - School Maternity Vila Nova Cachoeirinha, Sao Paulo, Sao Paulo, Brazil; Department of Immunology - University of Sao Paulo, Sao Paulo, Sao Paulo, Brazil.
authors.forename: Leandro
authors.initials: L
authors.lastname: De Oliveira
mesh_terms: D000328:Adult; D017668:Age of Onset; D005260:Female; D006801:Humans; D008055:Lipids / Q000097:blood*; D009765:Obesity / Q000097:blood* / Q000150:complications / Q000473:pathology; D010920:Placenta / Q000378:metabolism* / Q000473:pathology; D011225:Pre-Eclampsia / Q000097:blood* / Q000209:etiology / Q000473:pathology; D011247:Pregnancy; D018746:Systemic Inflammatory Response Syndrome / Q000097:blood / Q000150:complications / Q000473:pathology
chemical_list: D008055:Lipids