title: PCR based determination of mitochondrial DNA copy number in multiple species.
abstract: Mitochondrial DNA (mtDNA) copy number is a critical component of overall mitochondrial health. In this chapter, we describe methods for isolation of both mtDNA and nuclear DNA (nucDNA) and measurement of their respective copy numbers using quantitative PCR. Methods differ depending on the species and cell type of the starting material and availability of specific PCR reagents.
authors.affiliation: Nicholas School of the Environment, Duke University, 450 Research Dr., Durham, NC, 27708-0328, USA.
authors.forename: John P
authors.initials: JP
authors.lastname: Rooney
authors.forename: Ian T
authors.initials: IT
authors.lastname: Ryde
authors.forename: Laurie H
authors.initials: LH
authors.lastname: Sanders
authors.forename: Evan H
authors.initials: EH
authors.lastname: Howlett
authors.forename: Meryl D
authors.initials: MD
authors.lastname: Colton
authors.forename: Kaylyn E
authors.initials: KE
authors.lastname: Germ
authors.forename: Greg D
authors.initials: GD
authors.lastname: Mayer
authors.forename: J Timothy
authors.initials: JT
authors.lastname: Greenamyre
authors.forename: Joel N
authors.initials: JN
authors.lastname: Meyer
mesh_terms: D000818:Animals; D017173:Caenorhabditis elegans / Q000166:cytology / Q000235:genetics; D002138:Calibration; D002467:Cell Nucleus / Q000235:genetics; D004272:DNA, Mitochondrial / Q000235:genetics* / Q000302:isolation & purification; D018628:Gene Dosage; D006801:Humans; D051379:Mice; D016133:Polymerase Chain Reaction / Q000379:methods*; D051381:Rats
chemical_list: D004272:DNA, Mitochondrial