title: S100A12 expression in patients with primary biliary cirrhosis.
abstract: OBJECTIVE
  S100 calcium binding protein A12 (S100A12) has been supposed to be a pro-inflammatory factor associated with non-infectious inflammatory diseases. However, whether S100A12 is involved in the inflammatory process of primary biliary cirrhosis (PBC) has not been shown.
  
  
  METHODS
  The levels of S100A12 mRNA transcripts in peripheral mononuclear blood cells (PBMCs) of 66 Chinese patients with primary biliary cirrhosis (PBC), 62 healthy controls (HC) and 55 chronic hepatitis B (CHB) were measured by qRT-PCR. S100A12 serum concentrations in 34 PBC patients were measured by ELISA.
  
  
  RESULTS
  The levels of S100A12 mRNA transcripts in PBMCs of patients with PBC were significantly higher than healthy controls (p < 0.01) and that of patients with CHB (p < 0.01). Importantly, the levels of S100A12 mRNA in PBMCs and S100A12 protein levels in serum were positively correlated with biochemical indicators of bile duct and hepatocyte damage.
  
  
  CONCLUSIONS
  S100A12 might participate in the damage of biliary epithelial cells and hepatocytes in PBC, and analysis of S100A12 expression could be useful as a surrogate marker for the evaluation of PBC activity.
authors.affiliation: Department of Rheumatology and Clinical Immunology, Peking Union Medical College Hospital, Peking Union Medical College & Chinese Academy of Medical Sciences , Beijing , China .
authors.forename: Danxu
authors.initials: D
authors.lastname: Ma
authors.forename: Xi
authors.initials: X
authors.lastname: Li
authors.forename: Lei
authors.initials: L
authors.lastname: Zhang
authors.forename: Chuiwen
authors.initials: C
authors.lastname: Deng
authors.forename: Ting
authors.initials: T
authors.lastname: Zhang
authors.forename: Li
authors.initials: L
authors.lastname: Wang
authors.forename: Chaojun
authors.initials: C
authors.lastname: Hu
authors.forename: Yongzhe
authors.initials: Y
authors.lastname: Li
authors.forename: Fengchun
authors.initials: F
authors.lastname: Zhang
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001652:Bile Ducts / Q000378:metabolism / Q000473:pathology; D016022:Case-Control Studies; D005260:Female; D015870:Gene Expression; D019694:Hepatitis B, Chronic / Q000097:blood / Q000235:genetics* / Q000473:pathology / Q000821:virology; D022781:Hepatocytes / Q000378:metabolism / Q000473:pathology; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000378:metabolism* / Q000473:pathology / Q000821:virology; D008105:Liver Cirrhosis, Biliary / Q000097:blood / Q000235:genetics* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D012333:RNA, Messenger / Q000097:blood / Q000235:genetics*; D009418:S100 Proteins / Q000097:blood / Q000235:genetics*; D000067616:S100A12 Protein
chemical_list: D012333:RNA, Messenger; D009418:S100 Proteins; D000067616:S100A12 Protein; C090441:S100A12 protein, human
keywords: Enzyme-linked immunosorbent assay; S100 calcium binding protein A12; peripheral blood mononuclear cells; primary biliary cirrhosis; quantitative real-time reverse transcription polymerase chain reaction