title: Interaction proteomics reveals brain region-specific AMPA receptor complexes.
abstract: Fast excitatory synaptic transmission in the brain is mediated by glutamate acting on postsynaptic AMPA receptors. Recent studies have revealed a substantial number of AMPA receptor auxiliary proteins, which potentially contribute to the regulation of AMPA receptor trafficking, subcellular receptor localization, and receptor gating properties. Here we examined the AMPA receptor interactomes from cortex, hippocampus, and cerebellum by comprehensive interaction proteomics. The study reveals that AMPA receptor auxiliary proteins are engaged in distinct brain region-specific AMPA receptors subcomplexes, which might underlie brain region-specific differential regulation of AMPA receptor properties. Depending on the brain region, an interacting protein can be involved in an AMPA and a non-AMPA receptor complex.
authors.affiliation: Department of Molecular and Cellular Neurobiology, and ‡Department of Functional Genomics, Center for Neurogenomics and Cognitive Research, Neuroscience Campus Amsterdam, VU University , De Boelelaan 1085, 1081 HV Amsterdam, The Netherlands.
authors.forename: Ning
authors.initials: N
authors.lastname: Chen
authors.forename: Nikhil J
authors.initials: NJ
authors.lastname: Pandya
authors.forename: Frank
authors.initials: F
authors.lastname: Koopmans
authors.forename: Violeta
authors.initials: V
authors.lastname: Castelo-Székelv
authors.forename: Roel C
authors.initials: RC
authors.lastname: van der Schors
authors.forename: August B
authors.initials: AB
authors.lastname: Smit
authors.forename: Ka Wan
authors.initials: KW
authors.lastname: Li
mesh_terms: D000818:Animals; D015153:Blotting, Western; D001921:Brain / Q000378:metabolism*; D002352:Carrier Proteins / Q000378:metabolism; D002531:Cerebellum / Q000378:metabolism; D002540:Cerebral Cortex / Q000378:metabolism; D002853:Chromatography, Liquid; D006624:Hippocampus / Q000378:metabolism; D047468:Immunoprecipitation; D008810:Mice, Inbred C57BL; D046912:Multiprotein Complexes / Q000378:metabolism*; D011485:Protein Binding; D021122:Protein Subunits / Q000378:metabolism; D020543:Proteome / Q000378:metabolism; D040901:Proteomics / Q000379:methods*; D018091:Receptors, AMPA / Q000378:metabolism*; D053719:Tandem Mass Spectrometry
chemical_list: D002352:Carrier Proteins; D046912:Multiprotein Complexes; D021122:Protein Subunits; D020543:Proteome; D018091:Receptors, AMPA
keywords: AMPA receptor; brain; protein complex; proteomics; synapse