title: KLF8 is required for bladder cancer cell proliferation and migration.
abstract: Krüppel-like factor 8 (KLF8) belongs to the Sp/KLF family of transcription factors. Recently, it is affirmed that KLF8 plays an important role in the regulation of epithelial-mesenchymal transition, which is a key process that occurs during cancer metastasis. Although the overexpression of KLF8 has been observed in several types of human cancers, the functional role of KLF8 in human bladder cancer remains unknown. Here, we investigated the effects of KLF8 knockdown on bladder cancer cell proliferation and migration in vitro. Lentivirus-mediated small interfering RNA (siRNA) targeting KLF8 specifically downregulated its expression in T24 and BT5637 bladder cancer cells. Knockdown of KLF8 significantly inhibit cell proliferation and colony formation. Cell cycle analysis showed that knockdown of KLF8 arrested T24 cells in the G0/G1 phase. Moreover, cell migration was attenuated in T24 cells after KLF8 knockdown. Furthermore, knockdown of KLF8 resulted in a reduction in vimentin and N-cadherin expression and an increase in β-catenin expression. These results indicate that KLF8 plays a crucial role in proliferation and migration of bladder cancer cells, and inhibition of KLF8 by siRNA may provide a potential therapeutic approach for gene therapy in bladder cancer.
authors.affiliation: Department of Urology, Hospital of Heilongjiang Province, Harbin, People's Republic of China.
authors.forename: Kai
authors.initials: K
authors.lastname: Liang
authors.affiliation: Department of Urology, the First Affiliated Hospital, China Medical University, Shenyang, People's Republic of China.
authors.forename: Tao
authors.initials: T
authors.lastname: Liu
authors.affiliation: Department of Urology, Hospital of Heilongjiang Province, Harbin, People's Republic of China.
authors.forename: Ning
authors.initials: N
authors.lastname: Chu
authors.affiliation: Department of Urology, Hospital of Heilongjiang Province, Harbin, People's Republic of China.
authors.forename: Jian
authors.initials: J
authors.lastname: Kang
authors.affiliation: Department of Urology, Hospital of Heilongjiang Province, Harbin, People's Republic of China.
authors.forename: Rui
authors.initials: R
authors.lastname: Zhang
authors.affiliation: Department of Urology, Hospital of Heilongjiang Province, Harbin, People's Republic of China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Yu
authors.affiliation: Department of Urology, Hospital of Heilongjiang Province, Harbin, People's Republic of China.
authors.forename: Dongqi
authors.initials: D
authors.lastname: Li
authors.affiliation: Department of Urology, Hospital of Heilongjiang Province, Harbin, People's Republic of China.
authors.forename: Dexiang
authors.initials: D
authors.lastname: Lu
mesh_terms: D002453:Cell Cycle; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D015536:Down-Regulation / Q000235:genetics; D055785:Gene Knockdown Techniques; D006801:Humans; D051741:Kruppel-Like Transcription Factors; D016086:Lentivirus / Q000235:genetics; D009857:Oncogenes / Q000235:genetics; D034741:RNA, Small Interfering / Q000235:genetics; D012097:Repressor Proteins / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D001749:Urinary Bladder Neoplasms / Q000473:pathology*
chemical_list: C507402:KLF8 protein, human; D051741:Kruppel-Like Transcription Factors; D034741:RNA, Small Interfering; D012097:Repressor Proteins
keywords: KLF8; RNAi; bladder cancer; migration; proliferation