title: Pro-apoptotic activities of polyphenolics from açai (Euterpe oleracea Martius) in human SW-480 colon cancer cells.
abstract: This study aimed to evaluate the cell growth inhibition activity of açai (Euterpe oleracea Mart.) polyphenolic extract against colon cancer HT-29 and SW-480 cells and the nonmalignant CCD-18Co colon fibroblast cells. Results showed that açai polyphenolic extract (5-20 mg/L) inhibited preferentially the growth of SW-480 cells with no toxicity in CCD-18Co cells, and this was accompanied by reduction of H2O2-induced reactive oxygen species (ROS) generation. The mechanisms involved in SW-480 cell growth-inhibition by açai polyphenolic extract included the downregulation of NF-κB proinflammatory transcription factor and the nuclear factor-kappa B targets intracellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1). Furthermore, prooncogenic specificity proteins (Sp) were downregulated as well as Sp-targets Bcl-2, vascular endothelial growth factor, and survivin. This was accompanied by activation of mitochondrial proapoptotic pathway involving increase of cytochrome c, cleavage of caspase-3, and decrease of PARP-1. Results strongly suggest that açai polyphenolic extract has antiinflammatory and cytotoxic activities in colon cancer cells and can be effective as natural colon cancer chemopreventive agents.
authors.affiliation: a Department of Nutrition and Food Science , Texas A&M University , College Station , Texas , USA.
authors.forename: Manoela Maciel dos Santos
authors.initials: MM
authors.lastname: Dias
authors.forename: Giuliana
authors.initials: G
authors.lastname: Noratto
authors.forename: Hercia Stampini Duarte
authors.initials: HS
authors.lastname: Martino
authors.forename: Shirley
authors.initials: S
authors.lastname: Arbizu
authors.forename: Maria do Carmo Gouveia
authors.initials: Mdo C
authors.lastname: Peluzio
authors.forename: Stephen
authors.initials: S
authors.lastname: Talcott
authors.forename: Afonso Mota
authors.initials: AM
authors.lastname: Ramos
authors.forename: Susanne U
authors.initials: SU
authors.lastname: Mertens-Talcott
mesh_terms: D000893:Anti-Inflammatory Agents / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D003110:Colonic Neoplasms / Q000473:pathology*; D015536:Down-Regulation; D065748:Euterpe / Q000737:chemistry*; D019073:HT29 Cells; D006801:Humans; D006861:Hydrogen Peroxide / Q000009:adverse effects; D051034:Inhibitor of Apoptosis Proteins / Q000235:genetics / Q000378:metabolism; D018799:Intercellular Adhesion Molecule-1 / Q000235:genetics / Q000378:metabolism; D016328:NF-kappa B / Q000235:genetics / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology*; D000071137:Poly (ADP-Ribose) Polymerase-1; D011065:Poly(ADP-ribose) Polymerases / Q000235:genetics / Q000378:metabolism; D059808:Polyphenols / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism; D000077022:Survivin; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics / Q000378:metabolism
chemical_list: D000893:Anti-Inflammatory Agents; C107489:BIRC5 protein, human; C573420:ICAM1 protein, human; D051034:Inhibitor of Apoptosis Proteins; D016328:NF-kappa B; D010936:Plant Extracts; D059808:Polyphenols; D017382:Reactive Oxygen Species; D000077022:Survivin; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A; D018799:Intercellular Adhesion Molecule-1; D006861:Hydrogen Peroxide; C499320:PARP1 protein, human; D000071137:Poly (ADP-Ribose) Polymerase-1; D011065:Poly(ADP-ribose) Polymerases