title: Exercise preconditioning protects against spinal cord injury in rats by upregulating neuronal and astroglial heat shock protein 72.
abstract: The heat shock protein 72 (HSP 72) is a universal marker of stress protein whose expression can be induced by physical exercise. Here we report that, in a localized model of spinal cord injury (SCI), exercised rats (given pre-SCI exercise) had significantly higher levels of neuronal and astroglial HSP 72, a lower functional deficit, fewer spinal cord contusions, and fewer apoptotic cells than did non-exercised rats. pSUPER plasmid expressing HSP 72 small interfering RNA (SiRNA-HSP 72) was injected into the injured spinal cords. In addition to reducing neuronal and astroglial HSP 72, the (SiRNA-HSP 72) significantly attenuated the beneficial effects of exercise preconditioning in reducing functional deficits as well as spinal cord contusion and apoptosis. Because exercise preconditioning induces increased neuronal and astroglial levels of HSP 72 in the gray matter of normal spinal cord tissue, exercise preconditioning promoted functional recovery in rats after SCI by upregulating neuronal and astroglial HSP 72 in the gray matter of the injured spinal cord. We reveal an important function of neuronal and astroglial HSP 72 in protecting neuronal and astroglial apoptosis in the injured spinal cord. We conclude that HSP 72-mediated exercise preconditioning is a promising strategy for facilitating functional recovery from SCI.
authors.affiliation: Graduate Institute of Injury Prevention and Control, Taipei Medical University, Taipei 110, Taiwan. chengkuei.chang@gmail.com.
authors.forename: Cheng-Kuei
authors.initials: CK
authors.lastname: Chang
authors.affiliation: Physical Medicine and Rehabilitation Department, Chi Mei Medical Center, Tainan 710, Taiwan. ufan0101@ms22.hinet.net.
authors.forename: Willy
authors.initials: W
authors.lastname: Chou
authors.affiliation: Department of Biotechnology, Southern Taiwan University of Science and Technology, Tainan 710, Taiwan. hjlin.cm@msa.hinet.net.
authors.forename: Hung-Jung
authors.initials: HJ
authors.lastname: Lin
authors.affiliation: Department of Radiology, Chi Mei Medical Center, Liouying, Tainan 736, Taiwan. yichingh@mail2000.com.tw.
authors.forename: Yi-Ching
authors.initials: YC
authors.lastname: Huang
authors.affiliation: Department of Biotechnology, Southern Taiwan University of Science and Technology, Tainan 710, Taiwan. sunyu0408@yahoo.com.tw.
authors.forename: Ling-Yu
authors.initials: LY
authors.lastname: Tang
authors.affiliation: Department of Medical Research, Chi Mei Medical Center, Tainan 710, Taiwan. 891201@mail.chimei.org.tw.
authors.forename: Mao-Tsun
authors.initials: MT
authors.lastname: Lin
authors.affiliation: Department of Biotechnology, Southern Taiwan University of Science and Technology, Tainan 710, Taiwan. jessica@mail.stust.edu.tw.
authors.forename: Ching-Ping
authors.initials: CP
authors.lastname: Chang
mesh_terms: D000818:Animals; D017209:Apoptosis; D001253:Astrocytes / Q000378:metabolism / Q000473:pathology; D066128:Gray Matter / Q000378:metabolism / Q000473:pathology; D050884:HSP72 Heat-Shock Proteins / Q000032:analysis* / Q000235:genetics*; D008297:Male; D009474:Neurons / Q000378:metabolism / Q000473:pathology; D010805:Physical Conditioning, Animal; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D013116:Spinal Cord / Q000378:metabolism / Q000473:pathology*; D013119:Spinal Cord Injuries / Q000235:genetics* / Q000473:pathology / Q000517:prevention & control*; D015854:Up-Regulation
chemical_list: D050884:HSP72 Heat-Shock Proteins; D034741:RNA, Small Interfering