title: Sulforaphane suppresses cardiac hypertrophy by inhibiting GATA4/GATA6 expression and MAPK signaling pathways.
abstract: METHODS
  Sulforaphane (SFN) is a naturally occurring isothiocynate compound found in cruciferous vegetables. Here, we report the effect of SFN on cardiac hypertrophy and propose an underlying mechanism.
  
  
  RESULTS
  SFN suppresses cardiomyocyte hypertrophy induced by hypertrophic stimuli in vitro and in vivo. SFN suppresses the expression of fetal genes, including atrial natriuretic peptide, brain natriuretic peptide, and beta myosin heavy chain. We used an siRNA technique and atrial natriuretic peptide promoter with mutated GATA binding sites to demonstrate that SFN mediates cardiac hypertrophy by modulating transcription factors GATA4/6.
  
  
  CONCLUSIONS
  These results suggest that SFN has the potential to prevent cardiac hypertrophy by downregulating GATA4/6 and mitogen-activated protein kinase signaling pathways.
authors.affiliation: Cardiovascular Convergence Research Center, Chonnam National University Hospital, Gwangju, South Korea.
authors.forename: Hae Jin
authors.initials: HJ
authors.lastname: Kee
authors.forename: Gwi Ran
authors.initials: GR
authors.lastname: Kim
authors.forename: In Kyeom
authors.initials: IK
authors.lastname: Kim
authors.forename: Myung Ho
authors.initials: MH
authors.lastname: Jeong
mesh_terms: D000818:Animals; D006332:Cardiomegaly / Q000188:drug therapy*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D015536:Down-Regulation; D005333:Fetus; D050991:GATA4 Transcription Factor / Q000235:genetics / Q000378:metabolism*; D050993:GATA6 Transcription Factor / Q000235:genetics / Q000378:metabolism*; D057809:HEK293 Cells; D006801:Humans; D017879:Isothiocyanates / Q000494:pharmacology*; D020935:MAP Kinase Signaling System; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D020928:Mitogen-Activated Protein Kinases / Q000235:genetics / Q000378:metabolism; D018995:Myosin Heavy Chains; D011401:Promoter Regions, Genetic; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism; D051381:Rats; D013454:Sulfoxides
chemical_list: D050991:GATA4 Transcription Factor; D050993:GATA6 Transcription Factor; C084365:Gata4 protein, rat; C084364:Gata6 protein, rat; D017879:Isothiocyanates; D034741:RNA, Small Interfering; D013454:Sulfoxides; D020928:Mitogen-Activated Protein Kinases; D018995:Myosin Heavy Chains; C016766:sulforaphane
keywords: Cardiac hypertrophy; GATA binding factor 4; GATA binding factor 6; Mitogen-activated protein kinase signaling; Sulforaphane