title: Fine structure of neurally differentiated iPS cells generated from a multiple sclerosis (MS) patient: a case study.
abstract: We compared the characteristics of neural cells derived from induced pluripotent stem (iPS) cells from a patient with multiple sclerosis versus neurally differentiated control iPS cells of a healthy individual. The iPS cells were differentiated toward the oligodendrocyte lineage using a four-step protocol established for the differentiation of embryonic stem cells. The resulting cell population was immunostained on day 112 of differentiation for the presence of oligodendrocytes and analyzed by transmission electron microscopy (TEM). Both patient and control samples resembled a mixed population of neural cells rather than oligodendroglia of high purity, including neural stem cell-like cells and possibly oligodendrocytes demonstrable by TEM.
authors.affiliation: 1Australian Regenerative Medicine Institute,Monash University,Clayton,VIC 3800,Australia.
authors.forename: Daniella
authors.initials: D
authors.lastname: Herszfeld
authors.affiliation: 1Australian Regenerative Medicine Institute,Monash University,Clayton,VIC 3800,Australia.
authors.forename: Natalie L
authors.initials: NL
authors.lastname: Payne
authors.affiliation: 1Australian Regenerative Medicine Institute,Monash University,Clayton,VIC 3800,Australia.
authors.forename: Aude
authors.initials: A
authors.lastname: Sylvain
authors.affiliation: 1Australian Regenerative Medicine Institute,Monash University,Clayton,VIC 3800,Australia.
authors.forename: Guizhi
authors.initials: G
authors.lastname: Sun
authors.affiliation: 1Australian Regenerative Medicine Institute,Monash University,Clayton,VIC 3800,Australia.
authors.forename: Claude C
authors.initials: CC
authors.lastname: Bernard
authors.affiliation: 2Monash Micro Imaging,Monash University,Clayton,VIC 3800,Australia.
authors.forename: Joan
authors.initials: J
authors.lastname: Clark
authors.affiliation: 1Australian Regenerative Medicine Institute,Monash University,Clayton,VIC 3800,Australia.
authors.forename: Henry
authors.initials: H
authors.lastname: Sathananthan
mesh_terms: D002454:Cell Differentiation; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000502:physiology / Q000648:ultrastructure*; D046529:Microscopy, Electron, Transmission; D009103:Multiple Sclerosis; D058953:Neural Stem Cells / Q000502:physiology / Q000648:ultrastructure*