title: Induction of DJ-1 protects neuronal cells from isoflurane induced neurotoxicity.
abstract: Oxidative stress, mitochondrial dysfunction and neuronal apoptosis are thought to be major contributors of Isoflurane toxicity. However, the underlying mechanisms remain largely to be determined. DJ-1, a protein that is involved in the response to various kinds of stress, has shown its neuroprotective effects. Whether DJ-1 has a neuroprotective effect against isoflurane-induced neurotoxocity is still unknown. In this study, we found that expression of DJ-1 is elevated in response to isoflurane treatment in human SH-SY5Y neuroblastoma cells. In order to clarify whether DJ-1 plays a potential role in isoflurane neurotoxicity or as a compensatory response for survival, we investigated the effects of DJ-1 silencing in isoflurane neurotoxicity. Our findings indicate that knockdown of DJ-1 promotes isoflurane-induced oxidative stress and mitochondrial dysfunction. Importantly, DJ-1 silencing was found to exacerbate isoflurane- induced apoptosis through modulation of mitochondria-dependent apoptosis pathways, thereby suggesting that induction of DJ-1 in response to isoflurane might act as a compensatory response for cell survival.
authors.affiliation: Department of Anesthesiology, Xiangya Hospital, Central South University, 87 Xiangya Road, 410008, Changsha, Hunan, China.
authors.forename: Wenjie
authors.initials: W
authors.lastname: Liu
authors.forename: Qulian
authors.initials: Q
authors.lastname: Guo
authors.forename: Xiaoling
authors.initials: X
authors.lastname: Hu
authors.forename: Liangyu
authors.initials: L
authors.lastname: Peng
authors.forename: Bin
authors.initials: B
authors.lastname: Zhou
mesh_terms: D016923:Cell Death / Q000187:drug effects / Q000502:physiology; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000096:biosynthesis*; D007530:Isoflurane / Q000633:toxicity*; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects / Q000502:physiology; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism*; D018696:Neuroprotective Agents / Q000378:metabolism; D015513:Oncogene Proteins / Q000096:biosynthesis*; D018384:Oxidative Stress / Q000187:drug effects / Q000502:physiology; D000071617:Protein Deglycase DJ-1
chemical_list: D047908:Intracellular Signaling Peptides and Proteins; D018696:Neuroprotective Agents; D015513:Oncogene Proteins; D007530:Isoflurane; C105131:PARK7 protein, human; D000071617:Protein Deglycase DJ-1