title: Differential diagnosis of fowlpox and infectious laryngotracheitis viruses in chicken diphtheritic manifestations by mono and duplex real-time polymerase chain reaction.
abstract: Infectious laryngotracheitis virus (ILTV) and fowlpox virus (FPV) cause diphtheritic lesions in chicken tracheas and can simultaneously infect the same bird. A differential molecular diagnostic test, the duplex real-time polymerase chain reaction, is now reported using ILTV and FPV vaccine viruses and clinical samples from chickens, either uninfected or naturally infected with ILTV or FPV, or with both viruses. The dual virus amplification by real-time polymerase chain reaction was demonstrated to behave similarly to monoplex amplification, in spite of the fact that the real-time exponential amplification plots of the vaccine viruses were more illustrative than those of the clinical samples.
authors.affiliation: a Division of Avian Diseases , Kimron Veterinary Institute , Bet Dagan , Israel.
authors.forename: Irit
authors.initials: I
authors.lastname: Davidson
authors.forename: Israel
authors.initials: I
authors.lastname: Raibstein
authors.forename: Amira
authors.initials: A
authors.lastname: Altory
mesh_terms: D000818:Animals; D002645:Chickens; D017931:DNA Primers / Q000235:genetics; D003937:Diagnosis, Differential; D005586:Fowlpox / Q000175:diagnosis*; D005587:Fowlpox virus / Q000235:genetics*; D006566:Herpesviridae Infections / Q000175:diagnosis / Q000662:veterinary*; D001352:Herpesvirus 1, Gallid / Q000235:genetics*; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary; D011201:Poultry Diseases / Q000175:diagnosis* / Q000821:virology*; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary*
chemical_list: D017931:DNA Primers