title: Technical note: comparison of the PrestoBlue and LDH release assays with the MTT assay for skin viability assessment.
abstract: MTT assay is the gold standard for assessing skin sample viability but it is time-consuming. Here we compared the MTT test with two other assays for the assessment of skin viability. The MTT, PrestoBlue (colorimetric method) and LDH release assays were applied to fresh and cryopreserved skin. Skin viability was considered proportional to the optical density values of the relevant analytes. PrestoBlue did not reliably distinguish between fresh and cryopreserved skin. The LDH release assay did not allow us to establish a viability index. We recommend the MTT assay for assessing skin viability.
authors.affiliation: Faculté de Médecine, Université Paris Descartes, Paris, France, sonia.gaucher@cch.aphp.fr.
authors.forename: Sonia
authors.initials: S
authors.lastname: Gaucher
authors.forename: Mohamed
authors.initials: M
authors.lastname: Jarraya
mesh_terms: D002470:Cell Survival / Q000502:physiology; D015925:Cryopreservation / Q000379:methods*; D066298:In Vitro Techniques; D007770:L-Lactate Dehydrogenase / Q000032:analysis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012867:Skin / Q000737:chemistry / Q000166:cytology*; D012879:Skin Physiological Phenomena; D013778:Tetrazolium Salts / Q000737:chemistry*; D014022:Tissue Survival / Q000502:physiology*
chemical_list: D013778:Tetrazolium Salts; D007770:L-Lactate Dehydrogenase