title: Transcriptional specialization of human dendritic cell subsets in response to microbial vaccines.
abstract: The mechanisms by which microbial vaccines interact with human APCs remain elusive. Herein, we describe the transcriptional programs induced in human DCs by pathogens, innate receptor ligands and vaccines. Exposure of DCs to influenza, Salmonella enterica and Staphylococcus aureus allows us to build a modular framework containing 204 transcript clusters. We use this framework to characterize the responses of human monocytes, monocyte-derived DCs and blood DC subsets to 13 vaccines. Different vaccines induce distinct transcriptional programs based on pathogen type, adjuvant formulation and APC targeted. Fluzone, Pneumovax and Gardasil, respectively, activate monocyte-derived DCs, monocytes and CD1c+ blood DCs, highlighting APC specialization in response to vaccines. Finally, the blood signatures from individuals vaccinated with Fluzone or infected with influenza reveal a signature of adaptive immunity activation following vaccination and symptomatic infections, but not asymptomatic infections. These data, offered with a web interface, may guide the development of improved vaccines.
authors.affiliation: Baylor Institute for Immunology Research, 3434 Live Oak Street, Dallas, Texas 75204, USA.
authors.forename: Romain
authors.initials: R
authors.lastname: Banchereau
authors.affiliation: Baylor Institute for Immunology Research, 3434 Live Oak Street, Dallas, Texas 75204, USA.
authors.forename: Nicole
authors.initials: N
authors.lastname: Baldwin
authors.affiliation: Baylor Institute for Immunology Research, 3434 Live Oak Street, Dallas, Texas 75204, USA.
authors.forename: Alma-Martina
authors.initials: AM
authors.lastname: Cepika
authors.affiliation: Baylor Institute for Immunology Research, 3434 Live Oak Street, Dallas, Texas 75204, USA.
authors.forename: Shruti
authors.initials: S
authors.lastname: Athale
authors.affiliation: Baylor Institute for Immunology Research, 3434 Live Oak Street, Dallas, Texas 75204, USA.
authors.forename: Yaming
authors.initials: Y
authors.lastname: Xue
authors.affiliation: Baylor Institute for Immunology Research, 3434 Live Oak Street, Dallas, Texas 75204, USA.
authors.forename: Chun I
authors.initials: CI
authors.lastname: Yu
authors.affiliation: Baylor Institute for Immunology Research, 3434 Live Oak Street, Dallas, Texas 75204, USA.
authors.forename: Patrick
authors.initials: P
authors.lastname: Metang
authors.affiliation: Baylor Institute for Immunology Research, 3434 Live Oak Street, Dallas, Texas 75204, USA.
authors.forename: Abhilasha
authors.initials: A
authors.lastname: Cheruku
authors.affiliation: Baylor Institute for Immunology Research, 3434 Live Oak Street, Dallas, Texas 75204, USA.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Berthier
authors.affiliation: Baylor Institute for Immunology Research, 3434 Live Oak Street, Dallas, Texas 75204, USA.
authors.forename: Ingrid
authors.initials: I
authors.lastname: Gayet
authors.affiliation: Baylor Institute for Immunology Research, 3434 Live Oak Street, Dallas, Texas 75204, USA.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Baylor Institute for Immunology Research, 3434 Live Oak Street, Dallas, Texas 75204, USA.
authors.forename: Marina
authors.initials: M
authors.lastname: Ohouo
authors.affiliation: Baylor Institute for Immunology Research, 3434 Live Oak Street, Dallas, Texas 75204, USA.
authors.forename: LuAnn
authors.initials: L
authors.lastname: Snipes
authors.affiliation: Baylor Institute for Immunology Research, 3434 Live Oak Street, Dallas, Texas 75204, USA.
authors.forename: Hui
authors.initials: H
authors.lastname: Xu
authors.affiliation: Baylor Institute for Immunology Research, 3434 Live Oak Street, Dallas, Texas 75204, USA.
authors.forename: Gerlinde
authors.initials: G
authors.lastname: Obermoser
authors.affiliation: Baylor Institute for Immunology Research, 3434 Live Oak Street, Dallas, Texas 75204, USA.
authors.forename: Derek
authors.initials: D
authors.lastname: Blankenship
authors.affiliation: Baylor Institute for Immunology Research, 3434 Live Oak Street, Dallas, Texas 75204, USA.
authors.forename: Sangkon
authors.initials: S
authors.lastname: Oh
authors.affiliation: Nationwide Children's Hospital, 700 Children's Drive, Columbus, Ohio 43205, USA.
authors.forename: Octavio
authors.initials: O
authors.lastname: Ramilo
authors.affiliation: 1] Benaroya Research Institute, 1201 9th Avenue, Seattle, Washington 98101, USA [2] Sidra Medical and Research Center, Doha, Qatar.
authors.forename: Damien
authors.initials: D
authors.lastname: Chaussabel
authors.affiliation: 1] Baylor Institute for Immunology Research, 3434 Live Oak Street, Dallas, Texas 75204, USA [2] Jackson Laboratory for Genomic Medicine, 263 Farmington Ave., Farmington, Connecticut 06030, USA.
authors.forename: Jacques
authors.initials: J
authors.lastname: Banchereau
authors.affiliation: 1] Baylor Institute for Immunology Research, 3434 Live Oak Street, Dallas, Texas 75204, USA [2] Jackson Laboratory for Genomic Medicine, 263 Farmington Ave., Farmington, Connecticut 06030, USA.
authors.forename: Karolina
authors.initials: K
authors.lastname: Palucka
authors.affiliation: Baylor Institute for Immunology Research, 3434 Live Oak Street, Dallas, Texas 75204, USA.
authors.forename: Virginia
authors.initials: V
authors.lastname: Pascual
mesh_terms: D000465:Algorithms; D000818:Animals; D018949:Antigens, CD1 / Q000378:metabolism; D000954:Antigens, Surface / Q000378:metabolism; D016000:Cluster Analysis; D016207:Cytokines / Q000378:metabolism; D003713:Dendritic Cells / Q000166:cytology* / Q000378:metabolism / Q000382:microbiology*; D004285:Dogs; D020869:Gene Expression Profiling; D006023:Glycoproteins / Q000378:metabolism; D006801:Humans; D053118:Influenza A Virus, H1N1 Subtype; D015847:Interleukin-4 / Q000378:metabolism; D061985:Madin Darby Canine Kidney Cells; D009000:Monocytes / Q000166:cytology / Q000378:metabolism; D025341:Principal Component Analysis; D019779:Salmonella enterica; D013211:Staphylococcus aureus; D018180:Thrombomodulin; D014158:Transcription, Genetic; D059467:Transcriptome; D014612:Vaccines / Q000737:chemistry*
chemical_list: D018949:Antigens, CD1; D000954:Antigens, Surface; C437944:CD1C protein, human; D016207:Cytokines; D006023:Glycoproteins; C546931:THBD protein, human; D018180:Thrombomodulin; D014612:Vaccines; D015847:Interleukin-4