title: Evaluation of the dimerization profiles of HER tyrosine kinases by time-resolved Förster resonance energy transfer (TR-FRET).
abstract: Activation of receptor tyrosine kinases (RTK), such as those belonging to the human epidermal growth factor receptor (HER) family, occurs only after receptor dimerization, which is a crucial step for cellular signal transduction and diversification. The HER family includes four members (EGFR/HER1, HER2, HER3, and HER4) that can homodimerize or heterodimerize. Here, we describe immunoassays based on time-resolved Förster resonance energy transfer (TR-FRET) to profile EGFR-EGFR, HER2-HER2, and EGFR-HER2 dimers directly in tumor samples.
authors.affiliation: ICM, Institut du Cancer Montpellier, Parc Euromédecine, 208 rue des apothicaires, 34298, Montpellier Cedex 5, France, evelyne.crapez@icm.unicancer.fr.
authors.forename: Evelyne
authors.initials: E
authors.lastname: Lopez-Crapez
authors.forename: Alexandre
authors.initials: A
authors.lastname: Ho-Pun-Cheung
authors.forename: Patrick
authors.initials: P
authors.lastname: Garnero
authors.forename: Hervé
authors.initials: H
authors.lastname: Bazin
mesh_terms: D000818:Animals; D061067:Antibodies, Monoclonal, Humanized / Q000737:chemistry; D045744:Cell Line, Tumor; D000068818:Cetuximab; D066246:ErbB Receptors / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D005260:Female; D031541:Fluorescence Resonance Energy Transfer; D005456:Fluorescent Dyes; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007118:Immunoassay; D051379:Mice; D010051:Ovarian Neoplasms / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D047428:Protein Kinase Inhibitors / Q000737:chemistry; D055503:Protein Multimerization; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction; D000068878:Trastuzumab; D023041:Xenograft Model Antitumor Assays
chemical_list: D061067:Antibodies, Monoclonal, Humanized; D000068818:Cetuximab; D066246:ErbB Receptors; D005456:Fluorescent Dyes; D047428:Protein Kinase Inhibitors; D018719:Erb-b2 Receptor Tyrosine Kinases; D000068878:Trastuzumab; C512478:EGFR protein, human; C508053:ERBB2 protein, human