title: GHSC70 is involved in the cellular entry of nervous necrosis virus.
abstract: Nervous necrosis virus (NNV) is a devastating pathogen of cultured marine fish and has affected more than 40 fish species. NNV belongs to the betanodaviruses of Nodaviridae and is a nonenveloped icosahedral particle with 2 single-stranded positive-sense RNAs. To date, knowledge regarding NNV entry into the host cell remains limited, and no NNV-specific receptor protein has been published. Using grouper fin cell line GF-1 and purified NNV capsid protein in a virus overlay protein binding assay (VOPBA), grouper heat shock cognate protein 70 (GHSC70) and grouper voltage-dependent anion selective channel protein 2 (GVDAC2) were investigated as NNV receptor protein candidates. We cloned and sequenced the genes for GHSC70 and GVDAC2 and expressed them in Escherichia coli for antiserum preparation. Knockdown of the expression of GHSC70 and GVDAC2 genes with specific short interfering RNAs (siRNAs) significantly downregulated viral RNA expression in NNV-infected GF-1 cells. By performing an immunoprecipitation assay, we confirmed that GHSC70 interacted with NNV capsid protein, while VDAC2 did not. Immunofluorescence staining and flow cytometry analysis revealed the presence of the GHSC70 protein on the cell surface. After a blocking assay, we detected the NNV RNA2 levels after 1 h of adsorption to GF-1 cells; the level was significantly lower in the cells pretreated with the GHSC70 antiserum than in nontreated cells. Therefore, we suggest that GHSC70 participates in the NNV entry of GF-1 cells, likely functioning as an NNV receptor or coreceptor protein.
  
  
  OBJECTIVE
  Fish nodavirus has caused mass mortality of more than 40 fish species worldwide and resulted in huge economic losses in the past 20 years. Among the four genotypes of fish nodaviruses, the red-spotted grouper nervous necrosis virus (RGNNV) genotype exhibits the widest host range. In our previous study, we developed monoclonal antibodies with high neutralizing efficiency against grouper NNV in GF-1 cells, indicating that NNV-specific receptor(s) may exist on the GF-1 cell membrane. However, no NNV receptor protein has been published. In this study, we found GHSC70 to be an NNV receptor (or coreceptor) candidate through VOBPA and provided several lines of evidence demonstrating that GHSC70 protein has a role in the NNV entry step of GF-1 cells. To the best of our knowledge, this is the first report identifying grouper HSC70 and its role in NNV entry into GF-1 cells.
authors.affiliation: Department of Life Science, National Taiwan University, Taipei, Taiwan.
authors.forename: Jui-Shin
authors.initials: JS
authors.lastname: Chang
authors.affiliation: Department of Life Science, National Taiwan University, Taipei, Taiwan shauchi@ntu.edu.tw.
authors.forename: Shau-Chi
authors.initials: SC
authors.lastname: Chi
mesh_terms: D000818:Animals; D001492:Bass; D036022:Capsid Proteins / Q000378:metabolism; D002460:Cell Line; D004926:Escherichia coli / Q000235:genetics; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D015870:Gene Expression; D055785:Gene Knockdown Techniques; D050883:HSC70 Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism*; D047468:Immunoprecipitation; D008565:Membrane Proteins / Q000032:analysis; D030381:Nodaviridae / Q000502:physiology*; D011485:Protein Binding; D025941:Protein Interaction Mapping; D011991:Receptors, Virus / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D053586:Virus Internalization; D050996:Voltage-Dependent Anion Channel 2 / Q000235:genetics / Q000378:metabolism
chemical_list: D036022:Capsid Proteins; D050883:HSC70 Heat-Shock Proteins; D008565:Membrane Proteins; D011991:Receptors, Virus; D011994:Recombinant Proteins; D050996:Voltage-Dependent Anion Channel 2