title: Influence of hydrophobic mismatch on the catalytic activity of Escherichia coli GlpG rhomboid protease.
abstract: Rhomboids comprise a broad family of intramembrane serine proteases that are found in a wide range of organisms and participate in a diverse array of biological processes. High-resolution structures of the catalytic transmembrane domain of the Escherichia coli GlpG rhomboid have provided numerous insights that help explain how hydrolytic cleavage can be achieved below the membrane surface. Key to this are observations that GlpG hydrophobic domain dimensions may not be sufficient to completely span the native lipid bilayer. This formed the basis for a model where hydrophobic mismatch Induces thinning of the local membrane environment to promote access to transmembrane substrates. However, hydrophobic mismatch also has the potential to alter the functional properties of the rhomboid, a possibility we explore in the current work. For this purpose, we purified the catalytic transmembrane domain of GlpG into phosphocholine or maltoside detergent micelles of varying alkyl chain lengths, and assessed proteolytic function with a model water-soluble substrate. Catalytic turnover numbers were found to depend on detergent alkyl chain length, with saturated chains containing 10-12 carbon atoms supporting maximal activity. Similar results were obtained in phospholipid bicelles, with no proteolytic activity being detected in longer-chain lipids. Although differences in thermal stability and GlpG oligomerization could not explain these activity differences, circular dichroism spectra suggest that mismatch gives rise to a small change in structure. Overall, these results demonstrate that hydrophobic mismatch can exert an inhibitory effect on rhomboid activity, with the potential for changes in local membrane environment to regulate activity in vivo.
authors.affiliation: Department of Chemistry, University of Ottawa, Ottawa, Ontario, Canada, K1N 6N5.
authors.forename: Alexander C Y
authors.initials: AC
authors.lastname: Foo
authors.forename: Brandon G R
authors.initials: BG
authors.lastname: Harvey
authors.forename: Jeff J
authors.initials: JJ
authors.lastname: Metz
authors.forename: Natalie K
authors.initials: NK
authors.lastname: Goto
mesh_terms: D002942:Circular Dichroism; D004268:DNA-Binding Proteins / Q000737:chemistry* / Q000378:metabolism*; D003902:Detergents / Q000737:chemistry / Q000378:metabolism; D010450:Endopeptidases / Q000737:chemistry* / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000737:chemistry* / Q000378:metabolism*; D057927:Hydrophobic and Hydrophilic Interactions; D008565:Membrane Proteins / Q000737:chemistry* / Q000378:metabolism*; D008823:Micelles; D008958:Models, Molecular; D017434:Protein Structure, Tertiary
chemical_list: D004268:DNA-Binding Proteins; D003902:Detergents; D029968:Escherichia coli Proteins; C102898:GlpG protein, E coli; D008565:Membrane Proteins; D008823:Micelles; D010450:Endopeptidases
keywords: GlpG; detergent micelles; hydrophobic mismatch; lipid-protein interactions; rhomboid protease