title: Toxicity of sulcotrione and grape marc on Vicia faba cells.
abstract: The cell toxicity of sulcotrione, a selective triketone herbicide, was evaluated on Vicia faba. Sulcotrione, trademark Mikado, grape marc, and mixtures of sulcotrione or Mikado with grape marc induced cell death. Addition of grape marc to either sulcotrione or Mikado enhanced cell death, especially with Mikado. Addition of grape marc to herbicides, sulcotrione, or Mikado resulted in different expression of genes usually associated with cell stress. Mixtures of grape marc and herbicides enhanced transcript accumulation for ubiquitin, hsp 70, and cytosolic superoxide dismutase, but did not change ascorbate peroxidase transcript accumulation. The results thus provide evidence that sulcotrione, Mikado, and mixtures with grape marc can trigger cell death and specific gene expressions. Cocktails of products with sulcotrione, such as commercial additives and grape marc, can modify biological features of pesticide. Moreover, grape marc differently enhanced cell toxicity of sulcotrione and Mikado, suggesting a synergy between pesticide products and grape marc.
authors.affiliation: Clermont Université, Université Blaise Pascal, UMR 547 PIAF , B.P. 10448, F-63000 Clermont-Ferrand, France.
authors.forename: Chaima
authors.initials: C
authors.lastname: Sta
authors.forename: Eric
authors.initials: E
authors.lastname: Goujon
authors.forename: Ezzeddine
authors.initials: E
authors.lastname: Ferjani
authors.forename: Gérard
authors.initials: G
authors.lastname: Ledoigt
mesh_terms: D016923:Cell Death / Q000187:drug effects; D003512:Cyclohexanones / Q000633:toxicity*; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D006540:Herbicides / Q000633:toxicity*; D008698:Mesylates / Q000633:toxicity*; D018384:Oxidative Stress / Q000187:drug effects; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D031307:Vicia faba / Q000166:cytology / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D027843:Vitis / Q000737:chemistry / Q000633:toxicity*; D014866:Waste Products / Q000009:adverse effects* / Q000032:analysis
chemical_list: D003512:Cyclohexanones; D006540:Herbicides; D008698:Mesylates; D010940:Plant Proteins; D014866:Waste Products; C079906:sulcotrione
keywords: Vicia faba; cell death; gene expression; grape marc; sulcotrione