title: Characterization of polyphenol oxidase activity in Ataulfo mango.
abstract: Crude extracts of Ataulfo exhibited polyphenol oxidase (PPO) activity with pyrogallol, 3-methylcatechol, catechol, gallic acid, and protocatechuic acid. The substrate dependent pH optima ranged from pH 5.4 to 6.4 with Michaelis-Menten constants between 0.84 ± 0.09 and 4.6 ± 0.7 mM measured in MES or phosphate buffers. The use of acetate buffers resulted in larger Michaelis-Menten constants, up to 14.62 ± 2.03 mM. Sodium ascorbate, glutathione, and kojic acid are promising inhibitors to prevent enzymatic browning in Ataulfo. PPO activity increased with ripeness and was always higher in the skin compared to the pulp. Sodium dodecyl sulphate (SDS) enhanced PPO activity, with pulp showing a stronger increase than skin. SDS-PAGE gels stained for catecholase activity showed multiple bands, with the most prominent bands at apparent molecular weights of 53, 112, and 144 kDa.
authors.affiliation: Department of Chemistry and Biochemistry, California State University East Bay, 25800 Carlos Bee Blvd., Hayward, CA 94542, USA.
authors.forename: Summervir
authors.initials: S
authors.lastname: Cheema
authors.affiliation: Department of Chemistry and Biochemistry, California State University East Bay, 25800 Carlos Bee Blvd., Hayward, CA 94542, USA. Electronic address: monika.sommerhalter@csueastbay.edu.
authors.forename: Monika
authors.initials: M
authors.lastname: Sommerhalter
mesh_terms: D001205:Ascorbic Acid / Q000737:chemistry / Q000378:metabolism; D004156:Catechol Oxidase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000378:metabolism; D005978:Glutathione / Q000737:chemistry / Q000378:metabolism; D007700:Kinetics; D031022:Mangifera / Q000201:enzymology*; D008970:Molecular Weight; D010936:Plant Extracts / Q000378:metabolism; D011485:Protein Binding; D011753:Pyrones / Q000737:chemistry / Q000378:metabolism; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry / Q000378:metabolism
chemical_list: D004791:Enzyme Inhibitors; D010936:Plant Extracts; D011753:Pyrones; D012967:Sodium Dodecyl Sulfate; C011890:kojic acid; D004156:Catechol Oxidase; D005978:Glutathione; D001205:Ascorbic Acid
keywords: Ataulfo mango; Enzyme; Enzyme activity; Polyphenol oxidase