title: Trace quantification of 1-triacontanol in beagle plasma by GC-MS/MS and its application to a pharmacokinetic study.
abstract: 1-Triacontanol (TA), a member of long chain fatty alcohol, has recently been received great attention owing to its antitumor activity. In this study, an accurate, sensitive and selective gas chromatography-tandem mass spectrometry method was developed and validated for the quantification of TA in beagle plasma using 1-octacosanal as the internal standard (IS) for the first time. With temperature programming, chromatographic separation was carried out on an HP-5MS column, using helium as carrier gas and argon as collision gas, both at a flow rate of 1 mL/min. TA was analyzed using positive ion electrospray ionization in multiple-reaction monitoring mode, with the precursor to product ion transitions of m/z 495.6 → 97.0 and m/z 467.5 → 97.0 for TA and the IS, respectively. The lower limit of quantitation, linearity, intra- and interday precision, accuracy, stability, extraction recovery and matrix effect of TA were within the acceptable limits. The validated method was successfully applied to a pharmacokinetic study of TA in beagles.
authors.affiliation: Center of Drug Metabolism and Pharmacokinetics, China Pharmaceutical University, Nanjing, 210009, China.
authors.forename: Chunfeng
authors.initials: C
authors.lastname: Wang
authors.forename: Ali
authors.initials: A
authors.lastname: Fan
authors.forename: Xiaojie
authors.initials: X
authors.lastname: Zhu
authors.forename: Yang
authors.initials: Y
authors.lastname: Lu
authors.forename: Shuhua
authors.initials: S
authors.lastname: Deng
authors.forename: Wenchao
authors.initials: W
authors.lastname: Gao
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.forename: Qi
authors.initials: Q
authors.lastname: Liu
authors.forename: Xijing
authors.initials: X
authors.lastname: Chen
mesh_terms: D000818:Animals; D004285:Dogs; D005233:Fatty Alcohols / Q000097:blood* / Q000493:pharmacokinetics; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D008297:Male; D053719:Tandem Mass Spectrometry / Q000379:methods
chemical_list: D005233:Fatty Alcohols; C047578:1-triacontanol
keywords: 1-triacontanol; GC-MS/MS; pharmacokinetics