title: Oligomycin A-induced inhibition of mitochondrial ATP-synthase activity suppresses boar sperm motility and in vitro capacitation achievement without modifying overall sperm energy levels.
abstract: Incubation of boar spermatozoa in a capacitation medium with oligomycin A, a specific inhibitor of the F0 component of the mitochondrial ATP synthase, induced an immediate and almost complete immobilisation of cells. Oligomycin A also inhibited the ability of spermatozoa to achieve feasible in vitro capacitation (IVC), as measured through IVC-compatible changes in motility patterns, tyrosine phosphorylation levels of the acrosomal p32 protein, membrane fluidity and the ability of spermatozoa to achieve subsequent, progesterone-induced in vitro acrosome exocytosis (IVAE). Both inhibitory effects were caused without changes in the rhythm of O2 consumption, intracellular ATP levels or mitochondrial membrane potential (MMP). IVAE was accompanied by a fast and intense peak in O2 consumption and ATP levels in control spermatozoa. Oligomycin A also inhibited progesterone-induced IVAE as well as the concomitant peaks of O2 consumption and ATP levels. The effect of oligomycin on IVAE was also accompanied by concomitant alterations in the IVAE-induced changes on intracellular Ca(2+) levels and MMP. Our results suggest that the oligomycin A-sensitive mitochondrial ATP-synthase activity is instrumental in the achievement of an adequate boar sperm motion pattern, IVC and IVAE. However, this effect seems not to be linked to changes in the overall maintenance of adequate energy levels in stages other than IVAE.
authors.affiliation: Department of Animal Medicine and Surgery, School of Veterinary Medicine, Autonomous University of Barcelona, E-08193 Bellaterra, Barcelona, Spain.
authors.forename: Laura
authors.initials: L
authors.lastname: Ramió-Lluch
authors.affiliation: Department of Animal Medicine and Surgery, School of Veterinary Medicine, Autonomous University of Barcelona, E-08193 Bellaterra, Barcelona, Spain.
authors.forename: Marc
authors.initials: M
authors.lastname: Yeste
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Barcelona, E-08028 Barcelona, Spain.
authors.forename: Josep M
authors.initials: JM
authors.lastname: Fernández-Novell
authors.affiliation: Department of Animal Medicine and Surgery, School of Veterinary Medicine, Autonomous University of Barcelona, E-08193 Bellaterra, Barcelona, Spain.
authors.forename: Efrén
authors.initials: E
authors.lastname: Estrada
authors.affiliation: Department of Animal Medicine and Surgery, School of Veterinary Medicine, Autonomous University of Barcelona, E-08193 Bellaterra, Barcelona, Spain.
authors.forename: Luiz
authors.initials: L
authors.lastname: Rocha
authors.affiliation: Department of Biochemistry and Molecular and Cell Biology, I. U. C. A., School of Veterinary Medicine, University of Zaragoza, E-50013 Zaragoza, Spain.
authors.forename: José A
authors.initials: JA
authors.lastname: Cebrián-Pérez
authors.affiliation: Department of Biochemistry and Molecular and Cell Biology, I. U. C. A., School of Veterinary Medicine, University of Zaragoza, E-50013 Zaragoza, Spain.
authors.forename: Teresa
authors.initials: T
authors.lastname: Muiño-Blanco
authors.affiliation: Institute of Biochemistry and Microbiology and Institute of Animal Science, Universidad Austral de Chile, Independencia 641, Valdivia, Chile.
authors.forename: Ilona I
authors.initials: II
authors.lastname: Concha
authors.affiliation: Institute of Biochemistry and Microbiology and Institute of Animal Science, Universidad Austral de Chile, Independencia 641, Valdivia, Chile.
authors.forename: Alfredo
authors.initials: A
authors.lastname: Ramírez
authors.affiliation: Department of Animal Medicine and Surgery, School of Veterinary Medicine, Autonomous University of Barcelona, E-08193 Bellaterra, Barcelona, Spain.
authors.forename: Joan E
authors.initials: JE
authors.lastname: Rodríguez-Gil
mesh_terms: D000177:Acrosome / Q000187:drug effects; D000818:Animals; D004734:Energy Metabolism / Q000187:drug effects; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D008297:Male; D025261:Mitochondrial Proton-Translocating ATPases / Q000037:antagonists & inhibitors*; D009840:Oligomycins / Q000494:pharmacology*; D010101:Oxygen Consumption / Q000187:drug effects; D013075:Sperm Capacitation / Q000187:drug effects* / Q000502:physiology; D013081:Sperm Motility / Q000187:drug effects*; D013094:Spermatozoa / Q000187:drug effects / Q000378:metabolism; D013552:Swine
chemical_list: D004791:Enzyme Inhibitors; D009840:Oligomycins; C031004:oligomycin A; D025261:Mitochondrial Proton-Translocating ATPases