title: Ganoderma lucidum polysaccharide exerts anti-tumor activity via MAPK pathways in HL-60 acute leukemia cells.
abstract: In this study, we investigated the anti-tumor activity both in vitro and in vivo of a polysaccharide obtained from Ganoderma lucidum on HL-60 acute myeloid leukemia cells, and focused on its targeting effect on mitogen-activated protein kinase (MAPK) pathways. It was found by the methods such as western blot and flow cytometry (FCM), that G. lucidum polysaccharide (GLP) blocked the extracellular signal-regulated kinase/MAPK signaling pathway, simultaneously activated p38 and JNK MAPK pathways, and therefore regulated their downstream genes and proteins, including p53, c-myc, c-fos, c-jun, Bcl-2, Bax, cleaved caspase-3 and cyclin D1. As a result, cycle arrest and apoptosis of HL-60 cells were induced. Therefore, GLP exerted anti-tumor activity via MAPK pathways in HL-60 acute leukemia cells.
authors.affiliation: a Department of Hematology, Wu Xi People's Hospital , Nanjing Medical University , Wuxi , People's Republic of China.
authors.forename: Guohua
authors.initials: G
authors.lastname: Yang
authors.affiliation: a Department of Hematology, Wu Xi People's Hospital , Nanjing Medical University , Wuxi , People's Republic of China.
authors.forename: Lei
authors.initials: L
authors.lastname: Yang
authors.affiliation: a Department of Hematology, Wu Xi People's Hospital , Nanjing Medical University , Wuxi , People's Republic of China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Zhuang
authors.affiliation: a Department of Hematology, Wu Xi People's Hospital , Nanjing Medical University , Wuxi , People's Republic of China.
authors.forename: Xifeng
authors.initials: X
authors.lastname: Qian
authors.affiliation: a Department of Hematology, Wu Xi People's Hospital , Nanjing Medical University , Wuxi , People's Republic of China.
authors.forename: Yunfeng
authors.initials: Y
authors.lastname: Shen
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000302:isolation & purification / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D015153:Blotting, Western; D002453:Cell Cycle / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D005260:Female; D018922:HL-60 Cells; D006801:Humans; D048031:JNK Mitogen-Activated Protein Kinases / Q000378:metabolism; D007938:Leukemia / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism*; D010766:Phosphorylation / Q000187:drug effects; D011134:Polysaccharides / Q000302:isolation & purification / Q000494:pharmacology*; D020940:Reishi / Q000737:chemistry*; D015398:Signal Transduction / Q000187:drug effects*; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism
chemical_list: D000970:Antineoplastic Agents; D011134:Polysaccharides; D048031:JNK Mitogen-Activated Protein Kinases; D020928:Mitogen-Activated Protein Kinases; D048051:p38 Mitogen-Activated Protein Kinases
keywords: Acute myeloid leukemia; Ganoderma lucidum polysaccharide; MAPK pathways