title: Studying glycosaminoglycan-protein interactions using capillary electrophoresis.
abstract: Methods for studying interactions between glycosaminoglycans (GAGs) and proteins have assumed considerable significance as their biological importance increases. Capillary electrophoresis (CE) is a powerful method to study these interactions due to its speed, high efficiency, and low sample/reagent consumption. In addition, CE works effectively under a wide range of physiologically relevant conditions. This chapter presents state-of-the-art on CE methods for studying GAG-protein interactions including affinity capillary electrophoresis (ACE), capillary zone electrophoresis (CZE), frontal analysis (FA)/frontal analysis continuous capillary electrophoresis (FACCE), and capillary electrokinetic chromatography (CEC) with detailed experimental protocols for ACE and CZE methods.
authors.affiliation: Department of Physical Sciences, Charleston Southern University, 9200 University Boulevard, North Charleston, SC, 29406, USA, aliang@csuniv.edu.
authors.forename: Aiye
authors.initials: A
authors.lastname: Liang
authors.forename: Umesh R
authors.initials: UR
authors.lastname: Desai
mesh_terms: D020374:Chromatography, Micellar Electrokinetic Capillary; D019075:Electrophoresis, Capillary / Q000379:methods*; D006025:Glycosaminoglycans / Q000378:metabolism*; D006495:Heparin, Low-Molecular-Weight / Q000378:metabolism; D011485:Protein Binding; D011506:Proteins / Q000378:metabolism*
chemical_list: D006025:Glycosaminoglycans; D006495:Heparin, Low-Molecular-Weight; D011506:Proteins