title: Knockdown of protein tyrosine phosphatase receptor U inhibits growth and motility of gastric cancer cells.
abstract: Protein tyrosine phosphatase receptor U (PTPRU) has been shown to be a tumor suppressor in colon cancer by dephosphorylating β-catenin and reducing the activation of β-catenin signaling. Here, we investigate the expression of PTPRU protein in gastric cancer cell lines, gastric cancer tissues and respective adjacent non-cancer tissues and find that the 130 kDa nuclear-localized PTPRU fragment is the main PTPRU isoform in gastric cancer cells, whereas the full-length PTPRU is relatively lowly expressed. The level of the 130 kDa PTPRU is higher in gastric cancer tissues than in adjacent non-cancer tissues. Knockdown of endogenous PTPRU in gastric cancer cells using lentivirus-delivered specific shRNA results in the attenuation of cell growth, migration, invasion and adhesion. Knockdown of PTPRU also inhibits tyrosine phosphorylation and transcriptional activity of β-catenin as well as levels of focal adhesion proteins and lysine methylation of histone H3. These results indicate that PTPRU is required for gastric cancer progression and may serve as a potential therapeutic target.
authors.affiliation: Shanghai Key Laboratory of New Drug Design, School of Pharmacy, East China University of Science and Technology Shanghai 200237, P. R. China.
authors.forename: Yongjie
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Shanghai Key Laboratory of New Drug Design, School of Pharmacy, East China University of Science and Technology Shanghai 200237, P. R. China.
authors.forename: Zhichuan
authors.initials: Z
authors.lastname: Zhu
authors.affiliation: Shanghai Key Laboratory of New Drug Design, School of Pharmacy, East China University of Science and Technology Shanghai 200237, P. R. China.
authors.forename: Zhiqi
authors.initials: Z
authors.lastname: Xiong
authors.affiliation: Shanghai Key Laboratory of New Drug Design, School of Pharmacy, East China University of Science and Technology Shanghai 200237, P. R. China.
authors.forename: Jing
authors.initials: J
authors.lastname: Zheng
authors.affiliation: Shanghai Key Laboratory of New Drug Design, School of Pharmacy, East China University of Science and Technology Shanghai 200237, P. R. China.
authors.forename: Zelan
authors.initials: Z
authors.lastname: Hu
authors.affiliation: Department of Gastrointestinal Surgery, Ningbo First Hospital Ningbo 315010, P. R. China.
authors.forename: Jiangfeng
authors.initials: J
authors.lastname: Qiu
mesh_terms: D002448:Cell Adhesion; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D015972:Gene Expression Regulation, Neoplastic; D055785:Gene Knockdown Techniques; D006657:Histones / Q000378:metabolism; D006801:Humans; D008239:Lysine; D008745:Methylation; D009361:Neoplasm Invasiveness; D010766:Phosphorylation; D034622:RNA Interference; D054623:Receptor-Like Protein Tyrosine Phosphatases, Class 2 / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction; D013274:Stomach Neoplasms / Q000201:enzymology* / Q000235:genetics / Q000473:pathology; D013997:Time Factors; D014158:Transcription, Genetic; D014162:Transfection; D014443:Tyrosine; D051176:beta Catenin / Q000378:metabolism
chemical_list: C495270:CTNNB1 protein, human; D006657:Histones; D051176:beta Catenin; D014443:Tyrosine; C516868:PTPRU protein, human; D054623:Receptor-Like Protein Tyrosine Phosphatases, Class 2; D008239:Lysine
keywords: PTPRU; gastric cancer; motility; proliferation; β-catenin