title: BN-PAGE-based approach to study thyroid hormones and mitochondrial function.
abstract: In recent years, a number of advancements have been made in the study of entire mitochondrial proteomes in both physiological and pathological conditions. Naturally occurring iodothyronines (i.e., T3 and T2) greatly influence mitochondrial oxidative capacity, directly or indirectly affecting the structure and function of the respiratory chain components. Blue native PAGE (BN-PAGE) can be used to isolate enzymatically active oxidative phosphorylation (OXPHOS) complexes in one step, allowing the clinical diagnosis of mitochondrial metabolism by monitoring OXPHOS catalytic and/or structural features. Protocols for isolating mammalian liver mitochondria and subsequent one-dimensional (1D) BN-PAGE will be described in relation to the impact of thyroid hormones on mitochondrial bioenergetics.
authors.affiliation: Dipartimento di Scienze e Tecnologie, Università degli Studi del Sannio, Via Port'Arsa 11, 82100, Benevento, Italy.
authors.forename: Elena
authors.initials: E
authors.lastname: Silvestri
authors.forename: Assunta
authors.initials: A
authors.lastname: Lombardi
authors.forename: Federica
authors.initials: F
authors.lastname: Cioffi
authors.forename: Fernando
authors.initials: F
authors.lastname: Goglia
mesh_terms: D000818:Animals; D002458:Cell Fractionation; D045222:Electron Transport Chain Complex Proteins / Q000737:chemistry / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D004734:Energy Metabolism; D008930:Mitochondria, Liver / Q000378:metabolism*; D010085:Oxidative Phosphorylation; D051381:Rats; D012394:Rosaniline Dyes / Q000378:metabolism; D012995:Solubility; D013194:Staining and Labeling; D013963:Thyroid Hormones / Q000378:metabolism*
chemical_list: D045222:Electron Transport Chain Complex Proteins; D012394:Rosaniline Dyes; D013963:Thyroid Hormones; C004692:coomassie Brilliant Blue