title: Dissecting the transcriptional response to elicitors in Vitis vinifera cells.
abstract: The high effectiveness of cyclic oligosaccharides like cyclodextrins in the production of trans-resveratrol in Vitis vinifera cell cultures is enhanced in the presence of methyl jasmonate. In order to dissect the basis of the interactions among the elicitation responses triggered by these two compounds, a transcriptional analysis of grapevine cell cultures treated with cyclodextrins and methyl jasmonate separately or in combination was carried out. The results showed that the activation of genes encoding enzymes from phenylpropanoid and stilbene biosynthesis induced by cyclodextrins alone was partially enhanced in the presence of methyl jasmonate, which correlated with their effects on trans-resveratrol production. In addition, protein translation and cell cycle regulation were more highly repressed in cells treated with cyclodextrins than in those treated with methyl jasmonate, and this response was enhanced in the combined treatment. Ethylene signalling was activated by all treatments, while jasmonate signalling and salicylic acid conjugation were activated only in the presence of methyl jasmonate and cyclodextrins, respectively. Moreover, the combined treatment resulted in a crosstalk between the signalling cascades activated by cyclodextrins and methyl jasmonate, which, in turn, provoked the activation of additional regulatory pathways involving the up-regulation of MYB15, NAC and WRKY transcription factors, protein kinases and calcium signal transducers. All these results suggest that both elicitors cause an activation of the secondary metabolism in detriment of basic cell processes like the primary metabolism or cell division. Crosstalk between cyclodextrins and methyl jasmonate-induced signalling provokes an intensification of these responses resulting in a greater trans-resveratrol production.
authors.affiliation: Department of Plant Biology, Faculty of Biology, University of Murcia, Murcia, Spain.
authors.forename: Lorena
authors.initials: L
authors.lastname: Almagro
authors.affiliation: Instituto de Ciencias de la Vid y del Vino (CSIC-Universidad de La Rioja-Gobierno de La Rioja), Complejo Científico Tecnológico, Logroño, Spain.
authors.forename: Pablo
authors.initials: P
authors.lastname: Carbonell-Bejerano
authors.affiliation: Department of Plant Biology, Faculty of Biology, University of Murcia, Murcia, Spain.
authors.forename: Sarai
authors.initials: S
authors.lastname: Belchí-Navarro
authors.affiliation: Department of Agrochemistry and Biochemistry, Faculty of Sciences, University of Alicante, Alicante, Spain.
authors.forename: Roque
authors.initials: R
authors.lastname: Bru
authors.affiliation: Instituto de Ciencias de la Vid y del Vino (CSIC-Universidad de La Rioja-Gobierno de La Rioja), Complejo Científico Tecnológico, Logroño, Spain.
authors.forename: José M
authors.initials: JM
authors.lastname: Martínez-Zapater
authors.affiliation: Instituto de Biología Agrícola de Mendoza (CONICET-Universidad Nacional de Cuyo), Facultad de Ciencias Agrarias, Mendoza, Argentina.
authors.forename: Diego
authors.initials: D
authors.lastname: Lijavetzky
authors.affiliation: Department of Plant Biology, Faculty of Biology, University of Murcia, Murcia, Spain.
authors.forename: María A
authors.initials: MA
authors.lastname: Pedreño
mesh_terms: D000085:Acetates / Q000494:pharmacology*; D003505:Cyclodextrins / Q000494:pharmacology*; D003517:Cyclopentanes / Q000494:pharmacology*; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D054883:Oxylipins / Q000494:pharmacology*; D010937:Plant Growth Regulators / Q000494:pharmacology*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D000077185:Resveratrol; D013267:Stilbenes / Q000378:metabolism; D059467:Transcriptome; D015854:Up-Regulation; D027843:Vitis / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*
chemical_list: D000085:Acetates; D003505:Cyclodextrins; D003517:Cyclopentanes; D054883:Oxylipins; D010937:Plant Growth Regulators; D010940:Plant Proteins; D013267:Stilbenes; C072239:methyl jasmonate; D000077185:Resveratrol