title: Generating a transgenic mouse line stably expressing human MHC surface antigen from a HAC carrying multiple genomic BACs.
abstract: The human artificial chromosome (HAC) vector is a promising tool to improve the problematic suppression and position effects of transgene expression frequently seen in transgenic cells and animals produced by conventional plasmid or viral vectors. We generated transgenic mice maintaining a single HAC vector carrying two genomic bacterial artificial chromosomes (BACs) from human HLA-DR loci (DRA and DRB1). Both transgenes on the HAC in transgenic mice exhibited tissue-specific expression in kidney, liver, lung, spleen, lymph node, bone marrow, and thymus cells in RT-PCR analysis. Stable functional expression of a cell surface HLA-DR marker from both transgenes, DRA and DRB1 on the HAC, was detected by flow cytometric analysis of splenocytes and maintained through at least eight filial generations. These results indicate that the de novo HAC system can allow us to manipulate multiple BAC transgenes with coordinated expression as a surface antigen through the generation of transgenic animals.
authors.affiliation: Laboratory of Cell Engineering, Department of Frontier Research, Kazusa DNA Research Institute, 2-6-7 Kazusa-Kamatari, Kisarazu, Chiba, 292-0818, Japan.
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Hasegawa
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Ishikura
authors.forename: Takanori
authors.initials: T
authors.lastname: Hasegawa
authors.forename: Takashi
authors.initials: T
authors.lastname: Watanabe
authors.forename: Junpei
authors.initials: J
authors.lastname: Suzuki
authors.forename: Manabu
authors.initials: M
authors.lastname: Nakayama
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Okamura
authors.forename: Tuneko
authors.initials: T
authors.lastname: Okazaki
authors.forename: Haruhiko
authors.initials: H
authors.lastname: Koseki
authors.forename: Osamu
authors.initials: O
authors.lastname: Ohara
authors.forename: Masashi
authors.initials: M
authors.lastname: Ikeno
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Masumoto
mesh_terms: D000818:Animals; D016466:CHO Cells; D022202:Chromosomes, Artificial, Bacterial; D022222:Chromosomes, Artificial, Human; D003412:Cricetulus; D005786:Gene Expression Regulation; D016678:Genome; D006684:HLA-DR Antigens / Q000235:genetics*; D006801:Humans; D051379:Mice; D008822:Mice, Transgenic / Q000235:genetics*; D009928:Organ Specificity; D019076:Transgenes
chemical_list: D006684:HLA-DR Antigens