title: Photopatterning of multifunctional hydrogels to direct adult neural precursor cells.
abstract: Matrix-metalloproteinase and photosensitive peptide units are combined with heparin and poly(ethylene glycol) into a light-sensitive multicomponent hydrogel material. Localized degradation of the hydrogel matrix allows the creation of defined spatial constraints and adhesive patterning for cells grown in culture. Using this matrix system, it is demonstrated that the degree of confinement determines the fate of neural precursor cells in vitro.
authors.affiliation: Leibniz Institute of Polymer Research Dresden, Institute of Biofunctional Polymer, Materials/Max Bergmann Center of Biomaterials, Hohe Str. 6, 01069, Dresden, Germany; Center for Regenerative Therapies Dresden (CRTD), Technische Universität Dresden, 01307, Dresden, Germany.
authors.forename: Mikhail V
authors.initials: MV
authors.lastname: Tsurkan
authors.forename: Richard
authors.initials: R
authors.lastname: Wetzel
authors.forename: Heidi R
authors.initials: HR
authors.lastname: Pérez-Hernández
authors.forename: Karolina
authors.initials: K
authors.lastname: Chwalek
authors.forename: Anna
authors.initials: A
authors.lastname: Kozlova
authors.forename: Uwe
authors.initials: U
authors.lastname: Freudenberg
authors.forename: Gerd
authors.initials: G
authors.lastname: Kempermann
authors.forename: Yixin
authors.initials: Y
authors.lastname: Zhang
authors.forename: Andrés F
authors.initials: AF
authors.lastname: Lasagni
authors.forename: Carsten
authors.initials: C
authors.lastname: Werner
mesh_terms: D053687:Adult Stem Cells / Q000166:cytology* / Q000187:drug effects; D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D005898:Glass / Q000737:chemistry; D006493:Heparin / Q000737:chemistry; D020100:Hydrogels / Q000494:pharmacology*; D008027:Light; D051379:Mice; D058953:Neural Stem Cells / Q000166:cytology* / Q000187:drug effects; D011092:Polyethylene Glycols / Q000138:chemical synthesis / Q000737:chemistry
chemical_list: D020100:Hydrogels; D011092:Polyethylene Glycols; D006493:Heparin
keywords: biohybrid hydrogels; multilayer surfaces; neural precursor cells; photocleavable soft matter; photopatterning