title: Delay of ejaculation induced by Bersama engleriana in nicotinamide/streptozotocin-induced type 2 diabetic rats.
abstract: OBJECTIVE
  To evaluate the effects of aqueous and methanolic extracts of Bersama engleriana (B. engleriana) leaves on the expulsion phase of fictive ejaculation in nicotinamide/streptozotocin-induced type 2 diabetic male rats.
  
  
  METHODS
  The electromyographic activity of the bulbospongiosus muscles was recorded in urethane anaesthetized, spinal cord transected rats receiving dopamine (0.1 μmol/L/kg) intravenously, in the absence or presence of aqueous and methanolic extracts of B. engleriana (2.5, 10, 50, 60, 75 mg/kg). In another experiment, the pro-ejaculatory effect of dopamine (0.1 μmol/L/kg, i.v.) was monitored in rats orally pre-treated with the aqueous and methanolic extracts (60 mg/kg) of B. engleriana for 1 or 4 weeks.
  
  
  RESULTS
  Results of the study showed that the intravenous administration of B. engleriana did not provoke any contraction of the ejaculatory muscles whilst rhythmic and rapid contractions of the bulbospongiosus muscles accompanied sometimes by penis movement and expulsion of the urethral contents were recorded after dopamine application. The sequential treatment of animals with B. engleriana extracts (2.5-75.0 mg/kg) followed by dopamine (0.1 μmol/L/kg) resulted in a dose-dependent abolishment of the pro-ejaculatory response due to dopamine. However, in animals orally submitted to a daily gavage with B. engleriana extracts (60 mg/kg) for 1 or 4 weeks, the ejaculation stimulating effect of dopamine (0.1 μmol/L/kg) was significantly delayed (P<0.01) but not completely suppressed.
  
  
  CONCLUSIONS
  Present findings suggest the involvement of dopaminergic system in the activity of B. engleriana and further support its aphrodisiac potentials due to sterols and saponins revealed in this plant.
authors.affiliation: Animal Physiology and Phytopharmacology Laboratory, University of Dschang, Box 67 Dschang, Cameroon. Electronic address: pwatcho@yahoo.fr.
authors.forename: Pierre
authors.initials: P
authors.lastname: Watcho
authors.affiliation: Animal Physiology and Phytopharmacology Laboratory, University of Dschang, Box 67 Dschang, Cameroon.
authors.forename: Ulrich Carlos
authors.initials: UC
authors.lastname: Mbiakop
authors.affiliation: Animal Physiology and Phytopharmacology Laboratory, University of Dschang, Box 67 Dschang, Cameroon.
authors.forename: Hugues Gildas Achountsa
authors.initials: HG
authors.lastname: Jeugo
authors.affiliation: Animal Physiology and Phytopharmacology Laboratory, University of Dschang, Box 67 Dschang, Cameroon.
authors.forename: Modeste
authors.initials: M
authors.lastname: Wankeu
authors.affiliation: Animal Physiology and Phytopharmacology Laboratory, University of Dschang, Box 67 Dschang, Cameroon.
authors.forename: Telesphore Benoit
authors.initials: TB
authors.lastname: Nguelefack
authors.affiliation: Reproductive Behavior Laboratory, School of Veterinary Medicine and Animal Husbandry, Autonomous University of Tlaxcala, Tlaxcala P.O. BOX 90000, Mexico.
authors.forename: Miguel
authors.initials: M
authors.lastname: Carro-Juarez
authors.affiliation: Animal Physiology and Phytopharmacology Laboratory, University of Dschang, Box 67 Dschang, Cameroon.
authors.forename: Albert
authors.initials: A
authors.lastname: Kamanyi
keywords: Bersama engleriana; Diabetes; Ejaculation; Inhibition; Rat; Spinal cord