title: A monoclonal antibody against 47.2 kDa cell surface antigen prevents adherence and affects biofilm formation of Candida albicans.
abstract: Candida albicans is an opportunistic dimorphic pathogen that exists in both planktonic and biofilm phases causing deep-rooted infections in mainly immunocompromised patients. Antibodies are believed to play anti-Candida activity by different mechanisms, like inhibition of adhesion and neutralization of virulence-related antigens. Inhibition of adhesion is one of the important strategies to prevent Candida infections and biofilm formation. In this study, monoclonal antibody (MAb 7D7) against C. albicans biofilm cell surface antigen (47.2 kDa) was generated to determine the changes in adherence and viability of C. albicans. In this regard XTT assay was carried out in 30, 60, 90 min and 48 h (maturation time) time points using MAb 7D7 and it (MAb 7D7) was found to be effective against adhesion and the formation of C. albicans biofilm on polystyrene as well as monolayer of human epithelial cells (HeLa). This result may also prove to be a valuable addition to the reagents available to study C. albicans cell surface dynamics and interaction of the fungus with host cells.
authors.affiliation: Medical Mycology Lab, Division of Microbiology, CSIR-Central Drug Research Institute, Sitapur Road, Lucknow, 226 031, India.
authors.forename: Nripendra Nath
authors.initials: NN
authors.lastname: Mishra
authors.forename: Shakir
authors.initials: S
authors.lastname: Ali
authors.forename: Praveen K
authors.initials: PK
authors.lastname: Shukla
mesh_terms: D000908:Antibodies, Fungal / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000946:Antigens, Fungal / Q000737:chemistry / Q000276:immunology*; D000954:Antigens, Surface / Q000737:chemistry / Q000276:immunology; D018441:Biofilms / Q000254:growth & development*; D002176:Candida albicans / Q000276:immunology* / Q000502:physiology*; D002448:Cell Adhesion; D004783:Environmental Microbiology; D004847:Epithelial Cells / Q000382:microbiology; D005656:Fungal Proteins / Q000737:chemistry / Q000276:immunology*; D006367:HeLa Cells; D006801:Humans; D011137:Polystyrenes
chemical_list: D000908:Antibodies, Fungal; D000911:Antibodies, Monoclonal; D000946:Antigens, Fungal; D000954:Antigens, Surface; D005656:Fungal Proteins; D011137:Polystyrenes