title: Basic fibroblast growth factor gene transfection in repair of internal carotid artery aneurysm wall.
abstract: Surgery or interventional therapy has some risks in the treatment of cerebral aneurysm. We established an internal carotid artery aneurysm model by dripping elastase in the crotch of the right internal and external carotid arteries of New Zealand rabbits. Following model induction, lentivirus carrying basic fibroblast growth factor was injected through the ear vein. We found that the longer the action time of the lentivirus, the smaller the aneurysm volume. Moreover, platelet-derived growth factor expression in the aneurysm increased, but smooth muscle 22 alpha and hypertension-related gene 1 mRNA expression decreased. At 1, 2, 3, and 4 weeks following model establishment, following 1 week of injection of lentivirus carrying basic fibroblast growth factor, the later the intervention time, the more severe the blood vessel damage, and the bigger the aneurysm volume, the lower the smooth muscle 22 alpha and hypertension-related gene 1 mRNA expression. Simultaneously, platelet-derived growth factor expression decreased. These data suggest that recombinant lentivirus carrying basic fibroblast growth factor can repair damaged cells in the aneurysmal wall and inhibit aneurysm dynamic growth, and that the effect is dependent on therapeutic duration.
authors.affiliation: Department of Neurosurgery, Hospital Affiliated to Jiangsu University, Zhenjiang 215002, Jiangsu Province, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Jiao
authors.affiliation: Department of Neurosurgery, Hospital Affiliated to Jiangsu University, Zhenjiang 215002, Jiangsu Province, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Jiang
authors.affiliation: Department of Neurosurgery, Hospital Affiliated to Jiangsu University, Zhenjiang 215002, Jiangsu Province, China.
authors.forename: Jinghai
authors.initials: J
authors.lastname: Fang
authors.affiliation: Department of Neurosurgery, Hospital Affiliated to Jiangsu University, Zhenjiang 215002, Jiangsu Province, China.
authors.forename: Yinsheng
authors.initials: Y
authors.lastname: Deng
authors.affiliation: Department of Neurosurgery, Hospital Affiliated to Jiangsu University, Zhenjiang 215002, Jiangsu Province, China.
authors.forename: Zejun
authors.initials: Z
authors.lastname: Chen
authors.affiliation: Department of Neurosurgery, Hospital Affiliated to Jiangsu University, Zhenjiang 215002, Jiangsu Province, China.
authors.forename: Min
authors.initials: M
authors.lastname: Wu
keywords: aneurysm; basic fibroblast growth factor; brain injury; gene therapy; hypertension-related gene 1; lentivirus; neural regeneration; platelet-derived growth factor; smooth muscle 22 alpha; vascular smooth muscle cells