title: Identification of genes encoding photoconvertible (Class I) water-soluble chlorophyll-binding proteins from Chenopodium ficifolium.
abstract: Photoconvertible water-soluble chlorophyll-binding proteins, called Class I WSCPs, have been detected in Chenopodiaceae, Amaranthaceae and Polygonaceae plant species. To date, Chenopodium album WSCP (CaWSCP) is the only cloned gene encoding a Class I WSCP. In this study, we identified two cDNAs encoding Chenopodium ficifolium Class I WSCPs, CfWSCP1, and CfWSCP2. Sequence analyses revealed that the open reading frames of CfWSCP1 and CfWSCP2 were 585 and 588 bp, respectively. Furthermore, both CfWSCPs contain cystein2 and cystein30, which are essential for the chlorophyll-binding ability of CaWSCP. Recombinant CfWSCP1 and CfWSCP2, expressed in Escherichia coli as hexa-histidine fusion proteins (CfWSCP1-His and CfWSCP2-His), formed inclusion bodies; however, we were able to solubilize these using a buffer containing 8 M urea and then refold them by dialysis. The refolded CfWSCP1-His and CfWSCP2-His could bind chlorophylls and exhibited photoconvertibility, confirming that the cloned CfWSCPs are further examples of Class I WSCPs.
authors.affiliation: a Faculty of Science, Department of Biomolecular Science , Toho University , Funabashi , Japan.
authors.forename: Shigekazu
authors.initials: S
authors.lastname: Takahashi
authors.forename: Eriko
authors.initials: E
authors.lastname: Abe
authors.forename: Katsumi
authors.initials: K
authors.lastname: Nakayama
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Satoh
mesh_terms: D000595:Amino Acid Sequence; D027462:Chenopodium / Q000235:genetics*; D002734:Chlorophyll / Q000378:metabolism*; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*; D017421:Sequence Analysis; D012995:Solubility; D014867:Water / Q000737:chemistry*
chemical_list: D018076:DNA, Complementary; D010940:Plant Proteins; D014867:Water; D002734:Chlorophyll
keywords: Chenopodium ficifolium; Class I WSCP; DUF538; chlorophyll; water-soluble chlorophyll-binding protein