title: Experimental inoculation of Artibeus jamaicensis bats with dengue virus serotypes 1 or 4 showed no evidence of sustained replication.
abstract: Dengue is the most important mosquito-borne viral disease to humans. Bats are potential reservoirs for flaviviruses, including dengue virus (DENV). In this work, Artibeus jamaicensis bats were inoculated with two serotypes of DENV using different routes. For experimental inoculations (EI) 1 and 2, bats were inoculated subcutaneously or intraperitoneally with DENV-4; for EI-3 bats were inoculated intraperitoneally with DENV-1. Mock inoculated bats were kept as controls. In EI-4, bats were bitten by Aedes aegypti mosquitoes infected with DENV-1 or 4. Reverse transcription-polymerase chain reaction assays in plasma and spleen tissue collected from Day 1 to Days 9-17 after inoculation failed to reveal the presence of viral RNA in any of the samples. No evidence of circulating NS1 or specific anti-DENV IgG was detected in the plasma of the inoculated bats. These results indicate that A. jamaicensis bats are incapable of sustaining dengue virus replication and are unlikely to act as reservoirs for this virus.
authors.affiliation: Departament of Infectomics and Molecular Pathogenesis, Center for Research and Advanced Studies (CINVESTAV-IPN), Mexico City, Mexico; Center for Research on Infectious Diseases (CISEI), National Institute of Public Health (INSP), Cuernavaca, Mexico; Institute of Biology, Mexico City, Universidad Nacional Autónoma de México, Mexico.
authors.forename: Salomé
authors.initials: S
authors.lastname: Cabrera-Romo
authors.affiliation: Departament of Infectomics and Molecular Pathogenesis, Center for Research and Advanced Studies (CINVESTAV-IPN), Mexico City, Mexico; Center for Research on Infectious Diseases (CISEI), National Institute of Public Health (INSP), Cuernavaca, Mexico; Institute of Biology, Mexico City, Universidad Nacional Autónoma de México, Mexico.
authors.forename: Benito
authors.initials: B
authors.lastname: Recio-Tótoro
authors.affiliation: Departament of Infectomics and Molecular Pathogenesis, Center for Research and Advanced Studies (CINVESTAV-IPN), Mexico City, Mexico; Center for Research on Infectious Diseases (CISEI), National Institute of Public Health (INSP), Cuernavaca, Mexico; Institute of Biology, Mexico City, Universidad Nacional Autónoma de México, Mexico.
authors.forename: Ana C
authors.initials: AC
authors.lastname: Alcalá
authors.affiliation: Departament of Infectomics and Molecular Pathogenesis, Center for Research and Advanced Studies (CINVESTAV-IPN), Mexico City, Mexico; Center for Research on Infectious Diseases (CISEI), National Institute of Public Health (INSP), Cuernavaca, Mexico; Institute of Biology, Mexico City, Universidad Nacional Autónoma de México, Mexico.
authors.forename: Humberto
authors.initials: H
authors.lastname: Lanz
authors.affiliation: Departament of Infectomics and Molecular Pathogenesis, Center for Research and Advanced Studies (CINVESTAV-IPN), Mexico City, Mexico; Center for Research on Infectious Diseases (CISEI), National Institute of Public Health (INSP), Cuernavaca, Mexico; Institute of Biology, Mexico City, Universidad Nacional Autónoma de México, Mexico.
authors.forename: Rosa María
authors.initials: RM
authors.lastname: del Ángel
authors.affiliation: Departament of Infectomics and Molecular Pathogenesis, Center for Research and Advanced Studies (CINVESTAV-IPN), Mexico City, Mexico; Center for Research on Infectious Diseases (CISEI), National Institute of Public Health (INSP), Cuernavaca, Mexico; Institute of Biology, Mexico City, Universidad Nacional Autónoma de México, Mexico.
authors.forename: Victor
authors.initials: V
authors.lastname: Sánchez-Cordero
authors.affiliation: Departament of Infectomics and Molecular Pathogenesis, Center for Research and Advanced Studies (CINVESTAV-IPN), Mexico City, Mexico; Center for Research on Infectious Diseases (CISEI), National Institute of Public Health (INSP), Cuernavaca, Mexico; Institute of Biology, Mexico City, Universidad Nacional Autónoma de México, Mexico.
authors.forename: Ángel
authors.initials: Á
authors.lastname: Rodríguez-Moreno
authors.affiliation: Departament of Infectomics and Molecular Pathogenesis, Center for Research and Advanced Studies (CINVESTAV-IPN), Mexico City, Mexico; Center for Research on Infectious Diseases (CISEI), National Institute of Public Health (INSP), Cuernavaca, Mexico; Institute of Biology, Mexico City, Universidad Nacional Autónoma de México, Mexico jludert@cinvestav.mx.
authors.forename: Juan E
authors.initials: JE
authors.lastname: Ludert
mesh_terms: D000818:Animals; D001483:Base Sequence; D002685:Chiroptera / Q000821:virology; D017931:DNA Primers; D004279:DNA, Viral / Q000235:genetics; D003716:Dengue Virus / Q000145:classification / Q000302:isolation & purification* / Q000502:physiology; D016133:Polymerase Chain Reaction; D014779:Virus Replication
chemical_list: D017931:DNA Primers; D004279:DNA, Viral