title: Coronary stents seeded with human trophoblastic endovascular progenitor cells show accelerated strut coverage without excessive neointimal proliferation in a porcine model.
abstract: The success of percutaneous coronary intervention (PCI) has been limited by restenosis and stent thrombosis. Delayed or incomplete endothelial regeneration is believed to be a key factor responsible for these events. Developing a stent with an accelerated healing profile may be of benefit. We aimed to evaluate the feasibility and safety of seeding a bare metal stent (BMS) with human trophoblastic endovascular progenitor cells (hTEC) derived from human embryonic stem cells. A porcine coronary artery model was used to compare the rate and extent of endothelial regeneration and the degree of neointimal proliferation. Characterisation of hTEC confirmed a mixed progenitor and endothelial cell phenotype. The biodistribution and fate of hTEC were studied using radiolabelled 111Indium oxine and fluorescent in situ hybridisation. Scanning electron microscopy showed earlier endothelial coverage in hTEC-seeded stents as compared to similar BMS. hTEC-seeded BMS achieved complete stent coverage in three days. Quantitative coronary angiography, intravascular ultrasound assessment and histomorphometry showed no difference in neointimal hyperplasia between hTEC-seeded and control BMS. hTEC seeding of coronary stents is a novel and safe approach to accelerate endothelial regeneration without increasing neointimal proliferation.
authors.affiliation: Department of Cardiovascular Science, University of Sheffield, Sheffield, United Kingdom.
authors.forename: Tushar
authors.initials: T
authors.lastname: Raina
authors.forename: Javaid
authors.initials: J
authors.lastname: Iqbal
authors.forename: Nadine
authors.initials: N
authors.lastname: Arnold
authors.forename: Harry
authors.initials: H
authors.lastname: Moore
authors.forename: Behrouz
authors.initials: B
authors.lastname: Aflatoonian
authors.forename: Jim
authors.initials: J
authors.lastname: Walsh
authors.forename: Sam
authors.initials: S
authors.lastname: Whitehouse
authors.forename: Kadem
authors.initials: K
authors.lastname: Al-Lamee
authors.forename: Sheila
authors.initials: S
authors.lastname: Francis
authors.forename: Julian
authors.initials: J
authors.lastname: Gunn
mesh_terms: D000818:Animals; D049109:Cell Proliferation; D002478:Cells, Cultured; D017023:Coronary Angiography; D053595:Embryonic Stem Cells / Q000502:physiology; D004730:Endothelium, Vascular / Q000502:physiology*; D048091:Guided Tissue Regeneration; D008855:Microscopy, Electron, Scanning; D023421:Models, Animal; D058426:Neointima / Q000473:pathology; D013234:Stem Cells / Q000502:physiology*; D015607:Stents; D013552:Swine; D014327:Trophoblasts / Q000166:cytology; D018084:Ultrasonography, Interventional