title: Label-free and rapid electrical detection of hTSH with CMOS-compatible silicon nanowire transistor arrays.
abstract: Now a human thyroid stimulating hormone (hTSH) assay has been considered as a screening tool for thyroid disease. However, some existing methods employed for in-hospital diagnosis still suffer from labor-intensive experimental steps, and expensive instrumentation. It is of great significance to meet the ever growing demand for development of label-free, disposable, and low-cost productive hTSH detection biosensors. Herein, we demonstrate a novel sensing strategy for highly sensitive and selective immunodetection of hTSH by using a CMOS-compatible silicon nanowire field effect transistor (SiNW-FET) device. The SiNW chips were manufactured by a top-down approach, allowing for the possibility of low-cost and large-scale production. By using the antibody-functionalized SiNW-FET nanosensors, we performed the label-free and rapid electrical detection of hTSH without any nanoparticle conjugation or signal amplifications. The proposed SiNW biosensor could detect hTSH binding down to a concentration of at least 0.02 mIU/L (0.11 pM), which is more sensitive than other sensing techniques. We also investigated the influence of Debye screening with varied ionic strength on hTSH detection sensitivity, and real-time measurements on various concentrations of the diluted buffer. The simple, label-free, low-cost, and miniaturized SiNW-FET chip has a potential perspective in point-of-care diagnosis of thyroid disease.
authors.affiliation: Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences , 200050, Shanghai, China.
authors.forename: Na
authors.initials: N
authors.lastname: Lu
authors.forename: Pengfei
authors.initials: P
authors.lastname: Dai
authors.forename: Anran
authors.initials: A
authors.lastname: Gao
authors.forename: Jari
authors.initials: J
authors.lastname: Valiaho
authors.forename: Pasi
authors.initials: P
authors.lastname: Kallio
authors.forename: Yuelin
authors.initials: Y
authors.lastname: Wang
authors.forename: Tie
authors.initials: T
authors.lastname: Li
mesh_terms: D055910:Antibodies, Immobilized / Q000276:immunology; D015374:Biosensing Techniques; D004560:Electricity; D006801:Humans; D053770:Nanowires / Q000737:chemistry*; D009994:Osmolar Concentration; D012825:Silicon / Q000737:chemistry*; D013972:Thyrotropin / Q000032:analysis* / Q000276:immunology; D014173:Transistors, Electronic
chemical_list: D055910:Antibodies, Immobilized; D013972:Thyrotropin; D012825:Silicon
keywords: COMS-compatible; biosensor; human thyroid stimulating hormone (hTSH); label-free; silicon nanowires (SiNWs)