title: Drosophila casein kinase 2 (CK2) promotes warts protein to suppress Yorkie protein activity for growth control.
abstract: Drosophila Hippo signaling regulates Wts activity to phosphorylate and inhibit Yki in order to control tissue growth. CK2 is widely expressed and involved in a variety of signaling pathways. In this study we report that Drosophila CK2 promotes Wts activity to phosphorylate and inhibit Yki activity, which is independent of Hpo-induced Wts promotion. In vivo, CK2 overexpression suppresses hpo mutant-induced expanded (Ex) up-regulation and overgrowth phenotype, whereas it cannot affect wts mutant. Consistent with this, knockdown of CK2 up-regulates Hpo pathway target expression. We also found that Drosophila CK2 is essential for tissue growth as a cell death inhibitor as knockdown of CK2 in the developing disc induces severe growth defects as well as caspase3 signals. Taken together, our results uncover a dual role of CK2; although its major role is promoting cell survive, it may potentially be a growth inhibitor as well.
authors.affiliation: From the State Key Laboratory of Cell Biology and.
authors.forename: Lianxin
authors.initials: L
authors.lastname: Hu
authors.affiliation: From the State Key Laboratory of Cell Biology and.
authors.forename: Hongling
authors.initials: H
authors.lastname: Huang
authors.affiliation: From the State Key Laboratory of Cell Biology and.
authors.forename: Jinhui
authors.initials: J
authors.lastname: Li
authors.affiliation: From the State Key Laboratory of Cell Biology and.
authors.forename: Meng-Xin
authors.initials: MX
authors.lastname: Yin
authors.affiliation: From the State Key Laboratory of Cell Biology and.
authors.forename: Yi
authors.initials: Y
authors.lastname: Lu
authors.affiliation: From the State Key Laboratory of Cell Biology and.
authors.forename: Wenqing
authors.initials: W
authors.lastname: Wu
authors.affiliation: Key Laboratory of Systems Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Yue-Yang Road, Shanghai 200031, China and.
authors.forename: Rong
authors.initials: R
authors.lastname: Zeng
authors.affiliation: Department of Developmental Biology, University of Texas Southwestern Medical Center at Dallas, Dallas, Texas 75390.
authors.forename: Jin
authors.initials: J
authors.lastname: Jiang
authors.affiliation: From the State Key Laboratory of Cell Biology and.
authors.forename: Yun
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: From the State Key Laboratory of Cell Biology and rayzhang@sibcb.ac.cn.
authors.forename: Lei
authors.initials: L
authors.lastname: Zhang
mesh_terms: D000818:Animals; D047390:Casein Kinase II / Q000235:genetics / Q000378:metabolism*; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism*; D004331:Drosophila melanogaster; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics / Q000378:metabolism; D009154:Mutation; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D011494:Protein Kinases / Q000235:genetics / Q000378:metabolism*; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction / Q000502:physiology*; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism*; D000091102:YAP-Signaling Proteins; D000099317:Hippo Kinases
chemical_list: D047390:Casein Kinase II; D029721:Drosophila Proteins; D047908:Intracellular Signaling Peptides and Proteins; D009687:Nuclear Proteins; D011494:Protein Kinases; D017346:Protein Serine-Threonine Kinases; D015534:Trans-Activators; D000091102:YAP-Signaling Proteins; C503814:Yki protein, Drosophila; C093503:wts protein, Drosophila; C499474:hpo protein, Drosophila; D000099317:Hippo Kinases
keywords: Cell Signaling; Drosophila; Hippo Pathway; Phosphorylation; Tumor Promoter; Tumor Suppressor Gene