title: Transforming growth factor receptor type II (ec-TβR II) behaves as a halophile.
abstract: The members of transforming growth factor β family (TGF-β) are multifunctional proteins but their main role is to control cell proliferation and differentiation. Polypeptides of TGF-β family function by binding to two related, functionally distinct transmembrane receptor kinases, first to the type II (TβR II) followed by type I receptor (TβR I). The paper describes, in details, the stability of wt-ec-TβR II under different conditions. The stability of wt-ec-TβR II was observed at different pH and salt concentration using fluorescence spectroscopy. Stability of ec-TβR II decreases with decrease in pH. Interestingly, the addition of salt increases the stability of the TβRII at pH 5.0 as observed for halophiles. Computational analysis using DELPHI suggests that this is probably due to the decrease in repulsion between negatively charged residues at surface on the addition of salt. This is further confirmed by the change in the stability of receptor on mutation of some of the residues (D32A) at surface.
authors.forename: Komal
authors.initials: K
authors.lastname: Saini
authors.forename: M Ashhar I
authors.initials: MA
authors.lastname: Khan
authors.forename: Sumit
authors.initials: S
authors.lastname: Chakrapani
authors.forename: Shashank
authors.initials: S
authors.lastname: Deep
mesh_terms: D002942:Circular Dichroism; D006863:Hydrogen-Ion Concentration; D007477:Ions; D008958:Models, Molecular; D050505:Mutant Proteins / Q000737:chemistry; D009994:Osmolar Concentration; D011489:Protein Denaturation / Q000187:drug effects; D017346:Protein Serine-Threonine Kinases / Q000737:chemistry / Q000378:metabolism*; D055550:Protein Stability / Q000187:drug effects; D017433:Protein Structure, Secondary; D000077294:Receptor, Transforming Growth Factor-beta Type II; D018125:Receptors, Transforming Growth Factor beta / Q000737:chemistry / Q000378:metabolism*; D012965:Sodium Chloride / Q000494:pharmacology*; D013050:Spectrometry, Fluorescence; D055672:Static Electricity; D013816:Thermodynamics
chemical_list: D007477:Ions; D050505:Mutant Proteins; D018125:Receptors, Transforming Growth Factor beta; D012965:Sodium Chloride; D017346:Protein Serine-Threonine Kinases; D000077294:Receptor, Transforming Growth Factor-beta Type II