title: Reelin receptors ApoER2 and VLDLR are expressed in distinct spatiotemporal patterns in developing mouse cerebral cortex.
abstract: In mammalian developing brain, neuronal migration is regulated by a variety of signaling cascades, including Reelin signaling. Reelin is a glycoprotein that is mainly secreted by Cajal-Retzius neurons in the marginal zone, playing essential roles in the formation of the layered neocortex via its receptors, apolipoprotein E receptor 2 (ApoER2) and very low density lipoprotein receptor (VLDLR). However, the precise mechanisms by which Reelin signaling controls the neuronal migration process remain unclear. To gain insight into how Reelin signaling controls individual migrating neurons, we generated monoclonal antibodies against ApoER2 and VLDLR and examined the localization of Reelin receptors in the developing mouse cerebral cortex. Immunohistochemical analyses revealed that VLDLR is localized to the distal portion of leading processes in the marginal zone (MZ), whereas ApoER2 is mainly localized to neuronal processes and the cell membranes of multipolar cells in the multipolar cell accumulation zone (MAZ). These different expression patterns may contribute to the distinct actions of Reelin on migrating neurons during both the early and late migratory stages in the developing cerebral cortex.
authors.affiliation: Department of Anatomy, Keio University School of Medicine, Tokyo, 160-8582, Japan.
authors.forename: Yuki
authors.initials: Y
authors.lastname: Hirota
authors.forename: Ken-ichiro
authors.initials: K
authors.lastname: Kubo
authors.forename: Kei-ichi
authors.initials: K
authors.lastname: Katayama
authors.forename: Takao
authors.initials: T
authors.lastname: Honda
authors.forename: Takahiro
authors.initials: T
authors.lastname: Fujino
authors.forename: Tokuo T
authors.initials: TT
authors.lastname: Yamamoto
authors.forename: Kazunori
authors.initials: K
authors.lastname: Nakajima
mesh_terms: D000818:Animals; D015816:Cell Adhesion Molecules, Neuronal / Q000378:metabolism; D002540:Cerebral Cortex / Q000254:growth & development* / Q000378:metabolism*; D016326:Extracellular Matrix Proteins / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental / Q000235:genetics*; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D057809:HEK293 Cells; D006801:Humans; D026502:LDL-Receptor Related Proteins / Q000235:genetics / Q000378:metabolism*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008818:Mice, Neurologic Mutants; D009419:Nerve Tissue Proteins / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000378:metabolism; D011973:Receptors, LDL / Q000235:genetics / Q000378:metabolism*; D000091042:Reelin Protein; D012697:Serine Endopeptidases / Q000378:metabolism; D014162:Transfection
chemical_list: D015816:Cell Adhesion Molecules, Neuronal; D016326:Extracellular Matrix Proteins; D026502:LDL-Receptor Related Proteins; D009419:Nerve Tissue Proteins; D011956:Receptors, Cell Surface; D011973:Receptors, LDL; D000091042:Reelin Protein; C572338:Unc5d protein, mouse; C036053:VLDL receptor; C098773:low density lipoprotein receptor-related protein 8; D049452:Green Fluorescent Proteins; C000717127:RELN protein, human; C000717128:Reln protein, mouse; D012697:Serine Endopeptidases
keywords: ApoER2; RRID:AB_10862841; RRID:AB_2213003; RRID:AB_2253745; RRID:AB_2288612; RRID:AB_2336894; RRID:AB_2336895; RRID:AB_300798; RRID:AB_477577; RRID:AB_591819; RRID:AB_627679; RRID:AB_630921; RRID:IMSR_JAX:000235; RRID:IMSR_JAX:002529; Reelin; VLDLR; cerebral cortex; migration