title: Transactivating-transduction protein-polyethylene glycol modified liposomes traverse the blood-spinal cord and blood-brain barriers.
abstract: Naive liposomes can cross the blood-brain barrier and blood-spinal cord barrier in small amounts. Liposomes modified by a transactivating-transduction protein can deliver antibiotics for the treatment of acute bacterial infection-induced brain inflammation. Liposomes conjugated with polyethylene glycol have the capability of long-term circulation. In this study we prepared transactivating-transduction protein-polyethylene glycol-modified liposomes labeled with fluorescein isothiocyanate. Thus, liposomes were characterized by transmembrane, long-term circulation and fluorescence tracing. Uptake, cytotoxicity, and the ability of traversing blood-spinal cord and blood-brain barriers were observed following coculture with human breast adenocarcinoma cells (MCF-7). Results demonstrated that the liposomes had good biocompatibility, and low cytotoxicity when cocultured with human breast adenocarcinoma cells. Liposomes could traverse cell membranes and entered the central nervous system and neurocytes through the blood-spinal cord and blood-brain barriers of rats via the systemic circulation. These results verified that fluorescein isothiocyanate-modified transactivating-transduction protein-polyethylene glycol liposomes have the ability to traverse the blood-spinal cord and blood-brain barriers.
authors.affiliation: Department of Orthopedic Surgery, Tianjin Medical University General Hospital, Tianjin 300052, China.
authors.forename: Xianhu
authors.initials: X
authors.lastname: Zhou
authors.affiliation: Department of Orthopedic Surgery, Tianjin Medical University General Hospital, Tianjin 300052, China.
authors.forename: Chunyuan
authors.initials: C
authors.lastname: Wang
authors.affiliation: Department of Orthopedic Surgery, Tianjin Medical University General Hospital, Tianjin 300052, China.
authors.forename: Shiqing
authors.initials: S
authors.lastname: Feng
authors.affiliation: School of Material Science and Engineering, Tianjin University, Tianjin 300072, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Chang
authors.affiliation: Medicine College, Nankai University, Tianjin 300071, China.
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Kong
authors.affiliation: Department of Orthopedic Surgery, Tianjin Medical University General Hospital, Tianjin 300052, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Orthopedic Surgery, Tianjin Medical University General Hospital, Tianjin 300052, China.
authors.forename: Shijie
authors.initials: S
authors.lastname: Gao
keywords: blood-brain barrier; blood-spinal cord barrier; caudal vein; cytobiology; fluorescein isothiocyanate; human breast adenocarcinoma cells (MCF-7); liposomes; neural regeneration; polyethylene glycol; rat; transactivating-transduction protein