title: An animal model of peripheral nerve regeneration after the application of a collagen-polyvinyl alcohol scaffold and mesenchymal stem cells.
abstract: Extensive nerve injuries often leading to nerve gaps can benefit, besides the gold standard represented by autologous nerve grafts, by the inciting field of tissue engineering. To enhance the role of biomaterials in nerve regeneration, the nerve conduits are associated with Schwann or Schwann-like cells. In this study, we evaluated rat sciatic nerve regeneration, by using a biodegradable nerve guide composed of Collagen (COL) and Polyvinyl Alcohol (PVA), associated with mesenchymal stem cells (MSC). After the exposure of the rat sciatic nerve, a nerve gap was created by excising 1 cm of the nerve. Three experimental groups were used for nerve gap bridging: autografts, nerve conduits filled with medium culture and nerve conduits filled with MSC. The methods of sensory and motor assessment consisted of the functional evaluation of sciatic nerve recovery - toe-spread, pinprick tests and gastrocnemius muscle index (GMI). The histological and immunocytochemical analysis of the probes that were harvested from the repair site was performed at 12 weeks. Successful nerve regeneration was noted in all three groups at the end of the 12th week. The functional and immunocytochemical results suggested that COL-PVA tubes supported with mesenchymal stem cells could be considered similar to autologous nerve grafts in peripheral nerve regeneration, without the drawbacks of the last ones. The functional results were better for the autografts and the ultrastructural data were better for the nerve conduits, but there were not noticed any statistical differences.
authors.affiliation: Department of Plastic and Reconstructive Microsurgery, "Bagdasar-Arseni" Emergency Hospital, Bucharest, Romania; andamihai07@yahoo.com.
authors.forename: Silviu Adrian
authors.initials: SA
authors.lastname: Marinescu
authors.forename: Otilia
authors.initials: O
authors.lastname: Zărnescu
authors.forename: Ioana Ruxandra
authors.initials: IR
authors.lastname: Mihai
authors.forename: Carmen
authors.initials: C
authors.lastname: Giuglea
authors.forename: Ruxandra Diana
authors.initials: RD
authors.lastname: Sinescu
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000378:metabolism; D001854:Bone Marrow Cells / Q000473:pathology; D002417:Cattle; D002454:Cell Differentiation; D002478:Cells, Cultured; D003094:Collagen / Q000494:pharmacology*; D003720:Densitometry; D004195:Disease Models, Animal; D005260:Female; D005434:Flow Cytometry; D005780:Gelatin / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D009416:Nerve Regeneration / Q000187:drug effects*; D011142:Polyvinyl Alcohol / Q000494:pharmacology*; D017208:Rats, Wistar; D012584:Sciatic Nerve / Q000187:drug effects / Q000503:physiopathology* / Q000648:ultrastructure; D054457:Tissue Scaffolds / Q000737:chemistry*; D016138:Walking
chemical_list: D015703:Antigens, CD; D005780:Gelatin; D011142:Polyvinyl Alcohol; D003094:Collagen; D020778:Matrix Metalloproteinase 2