title: Analysis of nuclear organization with TANGO, software for high-throughput quantitative analysis of 3D fluorescence microscopy images.
abstract: The cell nucleus is a highly organized cellular organelle that contains the genome. An important step to understand the relationships between genome positioning and genome functions is to extract quantitative data from three-dimensional (3D) fluorescence imaging. However, such approaches are limited by the requirement for processing and analyzing large sets of images. Here we present a practical approach using TANGO (Tools for Analysis of Nuclear Genome Organization), an image analysis tool dedicated to the study of nuclear architecture. TANGO is a generic tool able to process large sets of images, allowing quantitative study of nuclear organization. In this chapter a practical description of the software is drawn in order to give an overview of its different concepts and functionalities. This description is illustrated with a precise example that can be performed step-by-step on experimental data provided on the website http://biophysique.mnhn.fr/tango/HomePage.
authors.affiliation: CNRS UMR7196, INSERM U565, Muséum National d'Histoire Naturelle, 37 rue Cuvier, Paris, 75005, France, ollion@mnhn.fr.
authors.forename: Jean
authors.initials: J
authors.lastname: Ollion
authors.forename: Julien
authors.initials: J
authors.lastname: Cochennec
authors.forename: François
authors.initials: F
authors.lastname: Loll
authors.forename: Christophe
authors.initials: C
authors.lastname: Escudé
authors.forename: Thomas
authors.initials: T
authors.lastname: Boudier
mesh_terms: D002467:Cell Nucleus / Q000235:genetics*; D002503:Centromere / Q000235:genetics; D007091:Image Processing, Computer-Assisted / Q000379:methods; D021621:Imaging, Three-Dimensional / Q000379:methods*; D020407:Internet; D008856:Microscopy, Fluorescence / Q000379:methods*; D012984:Software