title: Red fluorescent genetically encoded indicator for intracellular hydrogen peroxide.
abstract: Reactive oxygen species (ROS) are conserved regulators of numerous cellular functions, and overproduction of ROS is a hallmark of various pathological processes. Genetically encoded fluorescent probes are unique tools to study ROS production in living systems of different scale and complexity. However, the currently available recombinant redox sensors have green emission, which overlaps with the spectra of many other probes. Expanding the spectral range of recombinant in vivo ROS probes would enable multiparametric in vivo ROS detection. Here we present the first genetically encoded red fluorescent sensor for hydrogen peroxide detection, HyPerRed. The performance of this sensor is similar to its green analogues. We demonstrate the utility of the sensor by tracing low concentrations of H2O2 produced in the cytoplasm of cultured cells upon growth factor stimulation. Moreover, using HyPerRed we detect local and transient H2O2 production in the mitochondrial matrix upon inhibition of the endoplasmic reticulum Ca(2+) uptake.
authors.affiliation: Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, 117997 Moscow, Russia.
authors.forename: Yulia G
authors.initials: YG
authors.lastname: Ermakova
authors.affiliation: 1] Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, 117997 Moscow, Russia [2] NBIC, Moscow Institute of Physics and Technology, 123182 Moscow, Russia.
authors.forename: Dmitry S
authors.initials: DS
authors.lastname: Bilan
authors.affiliation: 1] Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, 117997 Moscow, Russia [2] NBIC, Moscow Institute of Physics and Technology, 123182 Moscow, Russia.
authors.forename: Mikhail E
authors.initials: ME
authors.lastname: Matlashov
authors.affiliation: Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, 117997 Moscow, Russia.
authors.forename: Natalia M
authors.initials: NM
authors.lastname: Mishina
authors.affiliation: Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, 117997 Moscow, Russia.
authors.forename: Ksenia N
authors.initials: KN
authors.lastname: Markvicheva
authors.affiliation: NBIC, Moscow Institute of Physics and Technology, 123182 Moscow, Russia.
authors.forename: Oksana M
authors.initials: OM
authors.lastname: Subach
authors.affiliation: NBIC, Moscow Institute of Physics and Technology, 123182 Moscow, Russia.
authors.forename: Fedor V
authors.initials: FV
authors.lastname: Subach
authors.affiliation: Department of Biophysics, School of Medicine, Saarland University, Homburg 66421, Germany.
authors.forename: Ivan
authors.initials: I
authors.lastname: Bogeski
authors.affiliation: Department of Biophysics, School of Medicine, Saarland University, Homburg 66421, Germany.
authors.forename: Markus
authors.initials: M
authors.lastname: Hoth
authors.affiliation: 1] NBIC, Moscow Institute of Physics and Technology, 123182 Moscow, Russia [2] Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, USA.
authors.forename: Grigori
authors.initials: G
authors.lastname: Enikolopov
authors.affiliation: 1] Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, 117997 Moscow, Russia [2] NBIC, Moscow Institute of Physics and Technology, 123182 Moscow, Russia.
authors.forename: Vsevolod V
authors.initials: VV
authors.lastname: Belousov
mesh_terms: D002118:Calcium / Q000737:chemistry; D003593:Cytoplasm / Q000737:chemistry; D004579:Electron Transport; D005456:Fluorescent Dyes / Q000737:chemistry; D057809:HEK293 Cells; D006367:HeLa Cells; D006801:Humans; D006861:Hydrogen Peroxide / Q000737:chemistry*; D007700:Kinetics; D008164:Luminescent Proteins / Q000737:chemistry*; D008856:Microscopy, Fluorescence; D008928:Mitochondria / Q000378:metabolism; D010084:Oxidation-Reduction; D017382:Reactive Oxygen Species / Q000737:chemistry*; D011994:Recombinant Proteins / Q000737:chemistry; D015398:Signal Transduction; D013053:Spectrophotometry; D013997:Time Factors; D000097573:Red Fluorescent Protein
chemical_list: D002118:Calcium; D005456:Fluorescent Dyes; D006861:Hydrogen Peroxide; D008164:Luminescent Proteins; D017382:Reactive Oxygen Species; D011994:Recombinant Proteins; D000097573:Red Fluorescent Protein