title: Longitudinal study of Salmonella spp., diarrheagenic Escherichia coli, Rotavirus, and Coronavirus isolated from healthy and diarrheic calves in a Brazilian dairy herd.
abstract: This prospective longitudinal study investigated the epidemiology of enteric disease associated with infections in calves aging up to 70 days. A total of 850 fecal samples were collected from 67 calves. Seventeen isolates of Salmonella spp. were recovered from feces of 11 calves (16.4%), and statistical analysis revealed no association between the presence of Salmonella spp. and clinical signs of diarrhea or age. Virulence factors of Escherichia coli were identified in 103 strains: eae (7), K99/STa (7), Stx1 (7), Stx1/eae (36), Stx1/Stx2/eae (2), Stx2 (43), and Stx2/eae (1). There was statistical association between diarrheic animals carrying E. coli Stx1/eae (+) in their feces at 2 and 4 weeks of age (P = 0.003) and E. coli Stx2 (+) at 5 weeks of age (P = 0.03). Rotavirus was detected in 49 (5.76%) fecal samples collected from 33 calves (49.2%). The presence of rotavirus was correlated with diarrheic feces (P < 0.0001) rather than feces with normal consistency. There was a significant relationship between age group and diarrhea (P = 0.001). Bovine coronavirus (BCoV) was detected in 93 fecal samples collected from 46 calves (68.6%). There was an association (P < 0.0001) between diarrheic animals positive for BCoV and age groups. The results demonstrate the importance of the pathogens studied in the etiology of diarrhea in calves.
authors.affiliation: Departamento de Medicina Veterinária Preventiva, Escola de Veterinária, Universidade Federal de Minas Gerais, Av. Antônio Carlos, 6627, Belo Horizonte, Minas Gerais, 31270-010, Brazil.
authors.forename: Fernanda Morcatti
authors.initials: FM
authors.lastname: Coura
authors.forename: Moisés Dias
authors.initials: MD
authors.lastname: Freitas
authors.forename: Juliane
authors.initials: J
authors.lastname: Ribeiro
authors.forename: Raquel Arruda
authors.initials: RA
authors.lastname: de Leme
authors.forename: Cecília
authors.initials: C
authors.lastname: de Souza
authors.forename: Amauri Alcindo
authors.initials: AA
authors.lastname: Alfieri
authors.forename: Elias Jorge
authors.initials: EJ
authors.lastname: Facury Filho
authors.forename: Antônio Último
authors.initials: AÚ
authors.lastname: de Carvalho
authors.forename: Marcos Xavier
authors.initials: MX
authors.lastname: Silva
authors.forename: Andrey Pereira
authors.initials: AP
authors.lastname: Lage
authors.forename: Marcos Bryan
authors.initials: MB
authors.lastname: Heinemann
mesh_terms: D000818:Animals; D001938:Brazil; D002417:Cattle; D002418:Cattle Diseases / Q000453:epidemiology; D017934:Coronavirus / Q000302:isolation & purification*; D018352:Coronavirus Infections / Q000662:veterinary*; D017938:Coronavirus, Bovine; D003967:Diarrhea / Q000662:veterinary*; D004926:Escherichia coli / Q000302:isolation & purification*; D004927:Escherichia coli Infections / Q000662:veterinary*; D005243:Feces; D008137:Longitudinal Studies; D011446:Prospective Studies; D012401:Rotavirus / Q000302:isolation & purification*; D012475:Salmonella / Q000302:isolation & purification*; D037521:Virulence Factors
chemical_list: D037521:Virulence Factors