title: A DNA vaccine encoding a chimeric allergen derived from major group 1 allergens of dust mite can be used for specific immunotherapy.
abstract: Immunization with DNA-based constructs has been shown to be against the antigen and the response is skewed in such a way as to ameliorate the symptoms of allergic disease. This approach is particularly useful in the treatment of allergic inflammatory diseases, such as asthma. The major group 1 allergen from house dust mites is one of the triggers of allergic asthma. This study explores whether a chimeric gene R8, derived from the major group 1 allergen of house dust mite species (Dermatophagoides farinae and Dermatophagoides pteronyssinus), can be expressed in Human Embryonic Kidney 293 cells (HEK 293 T) and whether such a construct can be used as a DNA vaccine in asthma therapy. The eukaryotic expression vector pcDNA3.1 was used to express the R8 molecule in HEK 293 T cells and successful expression of R8 was confirmed using a fluorescence microscope and western blot analysis. The efficacy of R8 as DNA vaccine was also assessed in a mouse asthma model. The in vivo data showed that R8 rectified the TH1/TH2 imbalance typical of allergic inflammation and stimulated the proliferation of regulatory T (Treg) cells. Immunization with the R8 construct also decreased serum allergen-specific IgE production in this mouse asthma model. Our findings suggest that R8 may be a feasible potential DNA vaccine for specific immunotherapy (SIT) in the treatment of allergic asthma.
authors.affiliation: Department of Endocrinology, Xuanwu Hospital of Capital Medical University 45 Changchun Street, Xicheng District, Beijing 100053, China.
authors.forename: Tong
authors.initials: T
authors.lastname: Sun
authors.affiliation: Department of Clinical Medicine, School of Clinical Medicine, Wannan Medical College 22 Wenchang Road, Wuhu 241002, China.
authors.forename: Kang
authors.initials: K
authors.lastname: Yin
authors.affiliation: Department of Clinical Medicine, School of Clinical Medicine, Wannan Medical College 22 Wenchang Road, Wuhu 241002, China.
authors.forename: Lu-Yi
authors.initials: LY
authors.lastname: Wu
authors.affiliation: Department of Clinical Medicine, School of Clinical Medicine, Wannan Medical College 22 Wenchang Road, Wuhu 241002, China.
authors.forename: Wen-Jie
authors.initials: WJ
authors.lastname: Jin
authors.affiliation: Department of Clinical Medicine, School of Clinical Medicine, Wannan Medical College 22 Wenchang Road, Wuhu 241002, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Clinical Medicine, School of Clinical Medicine, Wannan Medical College 22 Wenchang Road, Wuhu 241002, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Sheng
authors.affiliation: Department of Physiology, School of Basic Medicine, Wannan Medical College 22 Wenchang Road, Wuhu 241002, China.
authors.forename: Yu-Xin
authors.initials: YX
authors.lastname: Jiang
mesh_terms: D000485:Allergens / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology; D000818:Animals; D039741:Antigens, Dermatophagoides / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology*; D060829:Arthropod Proteins / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology*; D001249:Asthma / Q000097:blood / Q000175:diagnosis / Q000276:immunology / Q000628:therapy*; D015415:Biomarkers / Q000097:blood; D049109:Cell Proliferation; D003546:Cysteine Endopeptidases / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology*; D040001:Dermatophagoides farinae / Q000235:genetics / Q000276:immunology*; D040002:Dermatophagoides pteronyssinus / Q000235:genetics / Q000276:immunology*; D004195:Disease Models, Animal; D005260:Female; D057809:HEK293 Cells; D006801:Humans; D007114:Immunization; D007073:Immunoglobulin E / Q000097:blood; D007167:Immunotherapy / Q000379:methods*; D007273:Injections, Intramuscular; D008807:Mice, Inbred BALB C; D011498:Protein Precursors / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology; D011993:Recombinant Fusion Proteins / Q000276:immunology; D050378:T-Lymphocytes, Regulatory / Q000276:immunology; D018417:Th1 Cells / Q000276:immunology; D018418:Th2 Cells / Q000276:immunology; D014162:Transfection; D019444:Vaccines, DNA / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology*
chemical_list: D000485:Allergens; D039741:Antigens, Dermatophagoides; D060829:Arthropod Proteins; D015415:Biomarkers; C504325:ProDer p 1 allergen, Dermatophagoides pteronyssinus; D011498:Protein Precursors; D011993:Recombinant Fusion Proteins; D019444:Vaccines, DNA; D007073:Immunoglobulin E; D003546:Cysteine Endopeptidases; C451159:Dermatophagoides farinae antigen f 1
keywords: DNA vaccine; Dermatophagoides allergen 1 group; asthma; chimeric gene