title: Optimization of renewable pinene production from the conversion of macroalgae Saccharina latissima.
abstract: Enzymatic hydrolysis of Saccharina latissima with laminarinase was compared to hydrolysis with different combinations of cellulase and hemicellulase enzyme mixtures. The hemicellulase mixture resulted in similar release of glucose, while the cellulase mixture released 40% more glucose than laminarinase alone. The combination of a laminarinase augmented with a cellulase mixture resulted in a 53% increase of glucose release from S. latissima than laminarinase. Increasing biomass loading above 4% (w/v) reduced the sugar yield. Resulting macroalgae hydrolysates were used as a carbon source for the production of pinene, making use of a novel two plasmid Escherichia coli system. The macroalgal hydrolysates were suitable for the novel microbial production of pinene with no further treatment and/or purification.
authors.affiliation: Joint BioEnergy Institute, Emeryville, CA, USA; Sandia National Laboratories, Livermore, CA, USA. Electronic address: csscullin@lbl.gov.
authors.forename: Chessa
authors.initials: C
authors.lastname: Scullin
authors.affiliation: Sandia National Laboratories, Livermore, CA, USA. Electronic address: vnstavi@sandia.gov.
authors.forename: Vitalie
authors.initials: V
authors.lastname: Stavila
authors.affiliation: Statoil Research Center Trondheim, Trondheim, Norway. Electronic address: anisk@statoil.com.
authors.forename: Anita
authors.initials: A
authors.lastname: Skarstad
authors.affiliation: Joint BioEnergy Institute, Emeryville, CA, USA; Department of Chemical and Biomolecular Engineering, Department of Bioengineering, University of California, Berkeley, CA 94720, USA; Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA. Electronic address: jdkeasling@lbl.gov.
authors.forename: Jay D
authors.initials: JD
authors.lastname: Keasling
authors.affiliation: Joint BioEnergy Institute, Emeryville, CA, USA; Sandia National Laboratories, Livermore, CA, USA. Electronic address: basimmons@lbl.gov.
authors.forename: Blake A
authors.initials: BA
authors.lastname: Simmons
authors.affiliation: Joint BioEnergy Institute, Emeryville, CA, USA; Sandia National Laboratories, Livermore, CA, USA. Electronic address: seesing@sandia.gov.
authors.forename: Seema
authors.initials: S
authors.lastname: Singh
mesh_terms: D018533:Biomass; D001709:Biotechnology / Q000379:methods*; D050260:Carbohydrate Metabolism; D002241:Carbohydrates / Q000096:biosynthesis; D002480:Cellulase / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D006868:Hydrolysis; D039821:Monoterpenes / Q000378:metabolism*; D059205:Renewable Energy; D012621:Seasons; D012624:Seaweed / Q000378:metabolism*
chemical_list: D002241:Carbohydrates; D039821:Monoterpenes; D002480:Cellulase; D005947:Glucose
keywords: Cellulase; Laminarinase; Macroalgae; Renewable pinene; Saccharina latissima