title: Antioxidant/Prooxidant and antibacterial/probacterial effects of a grape seed extract in complex with lipoxygenase.
abstract: In an attempt to determine the antioxidant/prooxidant, antibacterial/probacterial action of flavan-3-ols and procyanidins from grape seeds, pure catechin (CS), and an aqueous grape seed extract (PE), were applied in the absence and presence of pure lipoxygenase (LS) or in extract (LE) to leucocyte culture, Escherichia coli B 41 and Brevibacterium linens, and observed whether there was any effect on lipid peroxidation, cytotoxicity, or growth rate. Short time periods of coincubation of cells with the polyphenols, followed by the exposure to LS and LE, revealed a high level of lipid peroxidation and a prooxidative effect. Longer coincubation and addition of LS and LE resulted in the reversal of the prooxidant action either to antioxidant activity for CS + LS and PE + LS or to the control level for CS + LE and PE + LE. Lipid peroxidation was significantly reduced when cells were exposed to polyphenols over a longer period. Longer exposure of E. coli to CS or PE followed by addition of LS for 3 h resulted in bactericidal activity. Significant stimulatory effect on microbial growth was observed for PE + LS and PE + LE treatments in B. linens, illustrating the potential probacterial activity in B. linens cultures. Lipoxygenase-polyphenols complex formation was found to be responsible for the observed effects.
authors.affiliation: Laboratory of Animal Biology, National Research Development Institute for Animal Biology and Nutrition (IBNA), Calea Bucureşti nr. 1, Baloteşti, 077015 Ilfov, Romania.
authors.forename: Veronica Sanda
authors.initials: VS
authors.lastname: Chedea
authors.affiliation: Research Center for Functional Genomics, Biomedicine and Translational Medicine, "Iuliu Hatieganu" University of Medicine and Pharmacy, 400 565 Cluj-Napoca, Romania.
authors.forename: Cornelia
authors.identifier: 0000-0002-3055-4747
authors.initials: C
authors.lastname: Braicu
authors.affiliation: Department of Microbiology-Immunology, University of Agricultural Sciences and Veterinary Medicine, 3-5 Manastur Street, 400372 Cluj-Napoca, Romania.
authors.forename: Flore
authors.initials: F
authors.lastname: Chirilă
authors.affiliation: School of Agricultural and Food Sciences, Jaramogi Oginga Odinga University of Science and Technology, P.O. Box 210, Bondo 40601, Kenya.
authors.forename: Henry Joseph Oduor
authors.initials: HJ
authors.lastname: Ogola
authors.affiliation: Laboratory of Animal Biology, National Research Development Institute for Animal Biology and Nutrition (IBNA), Calea Bucureşti nr. 1, Baloteşti, 077015 Ilfov, Romania.
authors.forename: Rodica Ştefania
authors.initials: RŞ
authors.lastname: Pelmuş
authors.affiliation: Laboratory of Animal Biology, National Research Development Institute for Animal Biology and Nutrition (IBNA), Calea Bucureşti nr. 1, Baloteşti, 077015 Ilfov, Romania.
authors.forename: Loredana Georgeta
authors.initials: LG
authors.lastname: Călin
authors.affiliation: Department of Chemistry and Biochemistry, University of Agricultural Sciences and Veterinary Medicine, 3-5 Manastur Street, 400372 Cluj-Napoca, Romania.
authors.forename: Carmen
authors.initials: C
authors.lastname: Socaciu
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology*; D001951:Brevibacterium / Q000187:drug effects / Q000254:growth & development; D002392:Catechin / Q000494:pharmacology; D019069:Cell Respiration / Q000187:drug effects; D004926:Escherichia coli / Q000187:drug effects / Q000254:growth & development; D056604:Grape Seed Extract / Q000494:pharmacology*; D006736:Horses; D007962:Leukocytes / Q000187:drug effects; D015227:Lipid Peroxidation / Q000187:drug effects; D008084:Lipoxygenase / Q000494:pharmacology*; D016877:Oxidants / Q000494:pharmacology*; D059808:Polyphenols / Q000494:pharmacology; D013025:Glycine max / Q000201:enzymology; D013056:Spectrophotometry, Ultraviolet; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D000975:Antioxidants; D056604:Grape Seed Extract; D016877:Oxidants; D059808:Polyphenols; D017392:Thiobarbituric Acid Reactive Substances; D002392:Catechin; D008084:Lipoxygenase