title: Facile synthesis of N, S-codoped fluorescent carbon nanodots for fluorescent resonance energy transfer recognition of methotrexate with high sensitivity and selectivity.
abstract: In this report, N, S-codoped fluorescent carbon nanodots (NSCDs) were prepared by a facile, simple, low-cost, and green thermal treatment of ammonium persulfate, glucose, and ethylenediamine. The as-prepared NSCDs displayed bright blue emission with a relatively high fluorescent quantum yield of 21.6%, good water solubility, uniform morphology, and excellent chemical stability, compared to pure CDs. The fluorescence of NSCDs can be significantly quenched by methotrexate (MTX) via fluorescence resonance energy transfer (FRET) between NSCDs and MTX, which was used for highly selective and sensitive detection of MTX with a wide linear range up to 50.0 μM and a low detection limit of 0.33 nM (S/N = 3). Moreover, this method was explored for practical detection of MTX in human serum with satisfied results.
authors.affiliation: College of Geography and Environmental Science, College of Chemistry and Life Science, Zhejiang Normal University, Jinhua 321004, China.
authors.forename: Weiping
authors.initials: W
authors.lastname: Wang
authors.affiliation: College of Geography and Environmental Science, College of Chemistry and Life Science, Zhejiang Normal University, Jinhua 321004, China.
authors.forename: Ya-Chun
authors.initials: YC
authors.lastname: Lu
authors.affiliation: College of Geography and Environmental Science, College of Chemistry and Life Science, Zhejiang Normal University, Jinhua 321004, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Huang
authors.affiliation: College of Geography and Environmental Science, College of Chemistry and Life Science, Zhejiang Normal University, Jinhua 321004, China. Electronic address: ajwang@zjun.cn.
authors.forename: Ai-Jun
authors.initials: AJ
authors.lastname: Wang
authors.affiliation: College of Geography and Environmental Science, College of Chemistry and Life Science, Zhejiang Normal University, Jinhua 321004, China.
authors.forename: Jian-Rong
authors.initials: JR
authors.lastname: Chen
authors.affiliation: College of Geography and Environmental Science, College of Chemistry and Life Science, Zhejiang Normal University, Jinhua 321004, China. Electronic address: jjfeng@zjnu.cn.
authors.forename: Jiu-Ju
authors.initials: JJ
authors.lastname: Feng
mesh_terms: D001774:Blood Chemical Analysis / Q000379:methods*; D002244:Carbon / Q000737:chemistry*; D003460:Crystallization / Q000379:methods; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D006801:Humans; D008727:Methotrexate / Q000097:blood*; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D045663:Quantum Dots; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D002244:Carbon; D008727:Methotrexate
keywords: Carbon nanodots; Fluorescence; Fluorescence resonance energy transfer; Methotrexate