title: Phytochemical, antioxidant and antibacterial potential of Elaeagnus kologa (Schlecht.) leaf.
abstract: OBJECTIVE
  To screen different solvent extracts of Elaeagnus kologa (E. kologa) leaf to determine the phytochemicals, potent antioxidant and antibacterial activity to find out the possible source of applied pharmaceutical formulations.
  
  
  METHODS
  Solvent extracts of leaf material were prepared using the Soxhlet apparatus. A study was performed on antioxidant activity of methanolic extract of leaf by 1-1-diphenyl-2-picrylhydrazyl method. The phenolic and flavonoid content of all the fractions were determined using high performance liquid chromatography. Leaves were also subjected to protein and carbohydrate test.
  
  
  RESULTS
  The total phenols, flavonoids were found to be high in petroleum ether as compare to other solvent fraction. The IC50 value of methanolic extract of the sample was 62.20 μg/mL which showed significant antibacterial activity against Bacillus subtilis (Gram-positive).
  
  
  CONCLUSIONS
  The present study suggests that the methanolic extract of E. kologa leaf possesses antioxidant and antibacterial properties. Such properties may be of great use in mitigating the detrimental effects of oxidative stress and reducing susceptibility to bacterial infection. Notably, extracts of E. kologa leaf also contain proteins and carbohydrates which add to its nutritional value.
authors.affiliation: Department of Biology, Stanford University, Stanford, USA.
authors.forename: Zoe
authors.initials: Z
authors.lastname: Merculieff
authors.affiliation: Department of Botany, Bangalore University, Bangalore-560056, India. Electronic address: Shubharani.bolar19@gmail.com.
authors.forename: Shubharani
authors.initials: S
authors.lastname: Ramnath
authors.affiliation: V Sivaram Research Foundation, # 132, 2nd Main Road, R R Layout, Bangalore, India.
authors.forename: Seethalakshmi Madhukar
authors.initials: SM
authors.lastname: Sankoli
authors.affiliation: Department of Botany, Bangalore University, Bangalore-560056, India.
authors.forename: Sivaram
authors.initials: S
authors.lastname: Venkataramegowda
authors.affiliation: Oregon State University, Corvallis, OR, USA.
authors.forename: Ganti S
authors.initials: GS
authors.lastname: Murthy
authors.affiliation: Division of Science and Mathematics, University of Minnesota, Morris, MN, USA; Native American Research Laboratory, Environmental Microbiology and Biochemistry Research Station (Bemidji, MN), USA.
authors.forename: Ruben Michael
authors.initials: RM
authors.lastname: Ceballos
keywords: 1-1-Diphenyl-2-picrylhydrazyl; Antibacterial; Antioxidant; Bacillus subtilis; Medicinal plant; Pseudomonas aeruginosa