title: Resonance assignment of the ligand-free cyclic nucleotide-binding domain from the murine ion channel HCN2.
abstract: Hyperpolarization activated and cyclic nucleotide-gated (HCN) ion channels as well as cyclic nucleotide-gated (CNG) ion channels are essential for the regulation of cardiac cells, neuronal excitability, and signaling in sensory cells. Both classes are composed of four subunits. Each subunit comprises a transmembrane region, intracellular N- and C-termini, and a C-terminal cyclic nucleotide-binding domain (CNBD). Binding of cyclic nucleotides to the CNBD promotes opening of both CNG and HCN channels. In case of CNG channels, binding of cyclic nucleotides to the CNBD is sufficient to open the channel. In contrast, HCN channels open upon membrane hyperpolarization and their activity is modulated by binding of cyclic nucleotides shifting the activation potential to more positive values. Although several high-resolution structures of CNBDs from HCN and CNG channels are available, the gating mechanism for murine HCN2 channel, which leads to the opening of the channel pore, is still poorly understood. As part of a structural investigation, here, we report the complete backbone and side chain resonance assignments of the murine HCN2 CNBD with part of the C-linker.
authors.affiliation: Institute of Complex Systems, Structural Biochemistry (ICS-6), Forschungszentrum Jülich, 52425, Jülich, Germany.|Institut für Physikalische Biologie, Heinrich-Heine-Universität, 40225, Düsseldorf, Germany.
authors.forename: Claudia
authors.initials: C
authors.lastname: Börger
authors.affiliation: Institute of Complex Systems, Structural Biochemistry (ICS-6), Forschungszentrum Jülich, 52425, Jülich, Germany.
authors.forename: Sven
authors.initials: S
authors.lastname: Schünke
authors.affiliation: Institute of Complex Systems, Structural Biochemistry (ICS-6), Forschungszentrum Jülich, 52425, Jülich, Germany.|Institut für Physikalische Biologie, Heinrich-Heine-Universität, 40225, Düsseldorf, Germany.
authors.forename: Justin
authors.initials: J
authors.lastname: Lecher
authors.affiliation: Institute of Complex Systems, Structural Biochemistry (ICS-6), Forschungszentrum Jülich, 52425, Jülich, Germany.|Institut für Physikalische Biologie, Heinrich-Heine-Universität, 40225, Düsseldorf, Germany.
authors.forename: Matthias
authors.initials: M
authors.lastname: Stoldt
authors.affiliation: Department of Molecular Sensory Systems, Center of Advanced European Studies and Research, 53175, Bonn, Germany.|Pharma Technical Development Penzberg, Roche Diagnostics GmbH, Nonnenwald 2, 82377, Penzberg, Germany.
authors.forename: Friederike
authors.initials: F
authors.lastname: Winkhaus
authors.affiliation: Department of Molecular Sensory Systems, Center of Advanced European Studies and Research, 53175, Bonn, Germany.
authors.forename: U Benjamin
authors.initials: UB
authors.lastname: Kaupp
authors.affiliation: Institute of Complex Systems, Structural Biochemistry (ICS-6), Forschungszentrum Jülich, 52425, Jülich, Germany. d.willbold@fz-juelich.de.|Institut für Physikalische Biologie, Heinrich-Heine-Universität, 40225, Düsseldorf, Germany. d.willbold@fz-juelich.de.
authors.forename: Dieter
authors.initials: D
authors.lastname: Willbold
mesh_terms: D000818:Animals; D064428:Hyperpolarization-Activated Cyclic Nucleotide-Gated Channels / Q000737:chemistry* / Q000378:metabolism*; D008024:Ligands; D051379:Mice; D019906:Nuclear Magnetic Resonance, Biomolecular; D009712:Nucleotides, Cyclic / Q000378:metabolism*; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D066244:Proton Magnetic Resonance Spectroscopy
chemical_list: D064428:Hyperpolarization-Activated Cyclic Nucleotide-Gated Channels; D008024:Ligands; D009712:Nucleotides, Cyclic
keywords: Apo state; HCN channel; Heteronuclear NMR; cAMP