title: Irisin promotes human umbilical vein endothelial cell proliferation through the ERK signaling pathway and partly suppresses high glucose-induced apoptosis.
abstract: Irisin is a newly discovered myokine that links exercise with metabolic homeostasis. It is involved in modest weight loss and improves glucose intolerance. However, the direct effects and mechanisms of irisin on vascular endothelial cells (ECs) are not fully understood. In the current study, we demonstrated that irisin promoted Human Umbilical Vein Endothelial Cell (HUVEC) proliferation. It was further demonstrated that this pro-proliferation effect was mediated by irisin-induced activation of extracellular signal-related kinase (ERK) signaling pathways. Inhibition of ERK signaling with U0126 decreased the pro-proliferation effect of irisin on HUVECs. It was also demonstrated that irisin reduced high glucose-induced apoptosis by up-regulating Bcl-2 expression and down-regulating Bax, Caspase-9 and Caspase-3 expression. In summary, these results suggested that irisin plays a novel role in sustaining endothelial homeostasis by promoting HUVEC proliferation via the ERK signaling pathway and protects the cell from high glucose-induced apoptosis by regulating Bcl-2,Bax and Caspase expression.
authors.affiliation: Center for Stem Cell & Regenerative Medicine, The Second Hospital of Shandong University, Jinan, P.R.China; Shandong University Qilu Hospital Research Center for Cell Therapy, Key Laboratory of Cardiovascular Remodeling and Function Research, Qilu Hospital of Shandong University, Jinan, P.R.China; Center for Reproductive Medicine, Zibo Maternal and Child health hospital, Zibo, P.R.China.
authors.forename: Haibo
authors.initials: H
authors.lastname: Song
authors.affiliation: Center for Stem Cell & Regenerative Medicine, The Second Hospital of Shandong University, Jinan, P.R.China; Shandong University Qilu Hospital Research Center for Cell Therapy, Key Laboratory of Cardiovascular Remodeling and Function Research, Qilu Hospital of Shandong University, Jinan, P.R.China.
authors.forename: Fei
authors.initials: F
authors.lastname: Wu
authors.affiliation: Center for Stem Cell & Regenerative Medicine, The Second Hospital of Shandong University, Jinan, P.R.China; Shandong University Qilu Hospital Research Center for Cell Therapy, Key Laboratory of Cardiovascular Remodeling and Function Research, Qilu Hospital of Shandong University, Jinan, P.R.China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Center for Stem Cell & Regenerative Medicine, The Second Hospital of Shandong University, Jinan, P.R.China; Shandong University Qilu Hospital Research Center for Cell Therapy, Key Laboratory of Cardiovascular Remodeling and Function Research, Qilu Hospital of Shandong University, Jinan, P.R.China.
authors.forename: Yuzhu
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Center for Stem Cell & Regenerative Medicine, The Second Hospital of Shandong University, Jinan, P.R.China.
authors.forename: Fang
authors.initials: F
authors.lastname: Wang
authors.affiliation: Center for Stem Cell & Regenerative Medicine, The Second Hospital of Shandong University, Jinan, P.R.China.
authors.forename: Miao
authors.initials: M
authors.lastname: Jiang
authors.affiliation: Center for Reproductive Medicine, Zibo Maternal and Child health hospital, Zibo, P.R.China.
authors.forename: Zhongde
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Shandong University Qilu Hospital Research Center for Cell Therapy, Key Laboratory of Cardiovascular Remodeling and Function Research, Qilu Hospital of Shandong University, Jinan, P.R.China.
authors.forename: Mingxiang
authors.initials: M
authors.lastname: Zhang
authors.affiliation: Center for Stem Cell & Regenerative Medicine, The Second Hospital of Shandong University, Jinan, P.R.China.
authors.forename: Shiwu
authors.initials: S
authors.lastname: Li
authors.affiliation: Center for Stem Cell & Regenerative Medicine, The Second Hospital of Shandong University, Jinan, P.R.China.
authors.forename: Lijun
authors.initials: L
authors.lastname: Yang
authors.affiliation: Shandong University Qilu Hospital Research Center for Cell Therapy, Key Laboratory of Cardiovascular Remodeling and Function Research, Qilu Hospital of Shandong University, Jinan, P.R.China.
authors.forename: Xing Li
authors.initials: XL
authors.lastname: Wang
authors.affiliation: Shandong University Qilu Hospital Research Center for Cell Therapy, Key Laboratory of Cardiovascular Remodeling and Function Research, Qilu Hospital of Shandong University, Jinan, P.R.China; Department of Cell Biology and Anatomy, University of South Carolina, Columbia, South Carolina, United States of America.
authors.forename: Taixing
authors.initials: T
authors.lastname: Cui
authors.affiliation: Center for Stem Cell & Regenerative Medicine, The Second Hospital of Shandong University, Jinan, P.R.China; Shandong University Qilu Hospital Research Center for Cell Therapy, Key Laboratory of Cardiovascular Remodeling and Function Research, Qilu Hospital of Shandong University, Jinan, P.R.China.
authors.forename: Dongqi
authors.initials: D
authors.lastname: Tang
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D002070:Butadienes / Q000494:pharmacology; D053148:Caspase 3 / Q000235:genetics / Q000378:metabolism; D053453:Caspase 9 / Q000235:genetics / Q000378:metabolism; D049109:Cell Proliferation / Q000187:drug effects; D048049:Extracellular Signal-Regulated MAP Kinases / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism; D005353:Fibronectins / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology*; D005786:Gene Expression Regulation; D005947:Glucose / Q000037:antagonists & inhibitors* / Q000378:metabolism / Q000494:pharmacology; D061307:Human Umbilical Vein Endothelial Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D009570:Nitriles / Q000494:pharmacology; D047428:Protein Kinase Inhibitors / Q000494:pharmacology; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology*; D015398:Signal Transduction; D051028:bcl-2-Associated X Protein / Q000235:genetics / Q000378:metabolism
chemical_list: C494826:BAX protein, human; C000595707:BCL2 protein, human; D002070:Butadienes; C577593:FNDC5 protein, human; D005353:Fibronectins; D009570:Nitriles; D047428:Protein Kinase Inhibitors; D019253:Proto-Oncogene Proteins c-bcl-2; D011994:Recombinant Proteins; C113580:U 0126; D051028:bcl-2-Associated X Protein; D048049:Extracellular Signal-Regulated MAP Kinases; D053148:Caspase 3; D053453:Caspase 9; D005947:Glucose