title: Complete genome sequence of the mitochondrial DNA of the sparkling enope squid, Watasenia scintillans.
abstract: The sparkling enope squid, Watasenia scintillans, is a deep-sea mollusk inhabiting the western part of the Pacific Ocean. It has the peculiar ability to illuminate its body without the involvement of other organisms. In this study, we extracted the brain DNA from a single squid female caught in the Japan Sea and determined the complete genome sequence of its mitochondrial DNA using the Illumina sequencing platform. The circular sequence is 20,089 bp in length. Using the next-generation sequencing data, we also estimated the mean copy number of mitochondria per cell in the brain to be 108 by comparing the depths of the read data in the nuclear and mitochondrial genomes. The haploid genome size was calculated to be 4.78 Gb. Six heteroplasmy sites were also identified, together with their allele frequencies, in this individual. Our methodology is shown to be useful in mitochondrion-related studies.
authors.affiliation: a Department of Maternal-Fetal Biology , National Research Institute for Child Health and Development , Tokyo , Japan .
authors.forename: Keiko
authors.initials: K
authors.lastname: Hayashi
authors.affiliation: a Department of Maternal-Fetal Biology , National Research Institute for Child Health and Development , Tokyo , Japan .|b Division of Life Sciences , Graduate School of Humanities and Sciences, Ochanomizu University , Tokyo , Japan .
authors.forename: Yuri L
authors.initials: YL
authors.lastname: Kawai
authors.affiliation: b Division of Life Sciences , Graduate School of Humanities and Sciences, Ochanomizu University , Tokyo , Japan .|c Center for Informational Biology, Ochanomizu University , Tokyo , Japan .|d National Institute of Genetics , Mishima , Japan .
authors.forename: Kei
authors.initials: K
authors.lastname: Yura
authors.affiliation: d National Institute of Genetics , Mishima , Japan .|e Japan Society for the Promotion of Science , Tokyo , Japan .
authors.forename: Masa-Aki
authors.initials: MA
authors.lastname: Yoshida
authors.affiliation: f Nagahama Institute of Bio-Science and Technology , Nagahama , Japan , and.
authors.forename: Atsushi
authors.initials: A
authors.lastname: Ogura
authors.affiliation: a Department of Maternal-Fetal Biology , National Research Institute for Child Health and Development , Tokyo , Japan .
authors.forename: Kenichiro
authors.initials: K
authors.lastname: Hata
authors.affiliation: a Department of Maternal-Fetal Biology , National Research Institute for Child Health and Development , Tokyo , Japan .
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Nakabayashi
authors.affiliation: c Center for Informational Biology, Ochanomizu University , Tokyo , Japan .|g Department of Systems BioMedicine , National Research Institute for Child Health and Development , Tokyo , Japan.
authors.forename: Kohji
authors.initials: K
authors.lastname: Okamura
mesh_terms: D000818:Animals; D020029:Base Pairing / Q000235:genetics; D001483:Base Sequence; D004272:DNA, Mitochondrial / Q000235:genetics*; D049832:Decapodiformes / Q000235:genetics*; D054629:Genome, Mitochondrial; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D000073336:Whole Genome Sequencing
chemical_list: D004272:DNA, Mitochondrial
keywords: Cephalopod; Watasenia scintillans; genome size; mollusk; sparkling enope squid