title: Effect of nitrogen source on growth and lipid accumulation in Scenedesmus abundans and Chlorella ellipsoidea.
abstract: Discovering microalgae strains containing a high lipid yield and adequate fatty acid composition is becoming a crucial fact in algae-oil factories. In this study, two unknown strains, named Scenedesmus abundans and Chlorella ellipsoidea, have been tested for their response to different nitrogen sources, in order to determine its influence in the production of lipids. For S. abundans, autotrophic culture with ammonium nitrate offers the maximum lipid yield, obtaining up to 3.55 mg L(-1) d(-1). For C. ellipsoidea, heterotrophic culture with ammonium nitrate has been shown to be the best condition, reaching a lipid production of 9.27 mg L(-1) d(-1). Moreover, fatty acid composition obtained from these cultures meets international biodiesel standards with an important amount of C18:1, achieving 70% of total fatty acids and thus representing a potential use of these two strains at an industrial scale.
authors.affiliation: Department of Chemical Engineering, University of Salamanca, P/Los Caídos s/n, Salamanca 37008, Spain.
authors.forename: Álvaro
authors.initials: Á
authors.lastname: González-Garcinuño
authors.affiliation: Department of Chemical Engineering, University of Salamanca, P/Los Caídos s/n, Salamanca 37008, Spain.
authors.forename: Antonio
authors.initials: A
authors.lastname: Tabernero
authors.affiliation: Department of Chemical Engineering, University of Salamanca, P/Los Caídos s/n, Salamanca 37008, Spain.
authors.forename: José Ma
authors.initials: JM
authors.lastname: Sánchez-Álvarez
authors.affiliation: Department of Chemical Engineering, University of Salamanca, P/Los Caídos s/n, Salamanca 37008, Spain. Electronic address: emvalle@usal.es.
authors.forename: Eva M
authors.initials: EM
authors.lastname: Martin Del Valle
authors.affiliation: Department of Chemical Engineering, University of Salamanca, P/Los Caídos s/n, Salamanca 37008, Spain.
authors.forename: Miguel A
authors.initials: MA
authors.lastname: Galán
mesh_terms: D056804:Biofuels; D018533:Biomass; D002708:Chlorella / Q000254:growth & development / Q000378:metabolism*; D003470:Culture Media / Q000737:chemistry; D005227:Fatty Acids / Q000378:metabolism; D007700:Kinetics; D050356:Lipid Metabolism; D009566:Nitrates / Q000378:metabolism; D009584:Nitrogen / Q000737:chemistry / Q000378:metabolism*; D044444:Scenedesmus / Q000254:growth & development / Q000378:metabolism*
chemical_list: D056804:Biofuels; D003470:Culture Media; D005227:Fatty Acids; D009566:Nitrates; D009584:Nitrogen; C006568:ammonium nitrate
keywords: Biodiesel; Microalgae; Nitrogen source