title: The effect of turmeric (Curcuma longa) extract on the functionality of the solute carrier protein 22 A4 (SLC22A4) and interleukin-10 (IL-10) variants associated with inflammatory bowel disease.
abstract: Inflammatory bowel disease (IBD) is a chronic relapsing disease. Genetic predisposition to the disease reduces an individual's capacity to respond appropriately to environmental challenges in the intestine leading to inappropriate inflammation. IBD patients often modify their diet to mitigate or reduce the severity of inflammation. Turmeric (Curcuma longa L., Zingiberaceae) has historically been used in Chinese, Hindu, and Ayurvedic medicine over several centuries to treat inflammatory disorders. To understand how turmeric may influence the consequences of a genetic predisposition to inappropriate inflammation, we used HEK293 cells to examine the in vitro capacity of turmeric extract and fractions to affect the functionality of two gene variants, solute carrier protein 22 A4 (SLC22A4, rs1050152) and interleukin-10 (IL-10, rs1800896) associated with IBD. We found that a turmeric extract and several chromatographically separated fractions beneficially affected the variants of SLC22A4 and IL-10 associated with IBD, by reducing inappropriate epithelial cell transport (SLC22A4, 503F) and increasing anti-inflammatory cytokine gene promoter activity (IL-10, -1082A). The effect of turmeric on the IL-10 variant was strongly associated with the curcumin content of the extract and its fractions.
authors.affiliation: Food Nutrition & Health, Food and Bio-Based Products, AgResearch Grasslands Research Centre, Palmerston North 4442, New Zealand. mark.mccann@agresearch.co.nz.
authors.forename: Mark J
authors.initials: MJ
authors.lastname: McCann
authors.affiliation: Food Nutrition & Health, Food and Bio-Based Products, AgResearch Grasslands Research Centre, Palmerston North 4442, New Zealand. sljohnston@xtra.co.nz.
authors.forename: Sarah
authors.initials: S
authors.lastname: Johnston
authors.affiliation: Food Nutrition & Health, Food and Bio-Based Products, AgResearch Grasslands Research Centre, Palmerston North 4442, New Zealand. kerri.reilly@agresearch.co.nz.
authors.forename: Kerri
authors.initials: K
authors.lastname: Reilly
authors.affiliation: Food Nutrition & Health, Food and Bio-Based Products, AgResearch Grasslands Research Centre, Palmerston North 4442, New Zealand. xuejing.men@agresearch.co.nz.
authors.forename: Xuejing
authors.initials: X
authors.lastname: Men
authors.affiliation: Nutrigenomics New Zealand, Plant and Food Research Ltd., Private Bag 92169, Auckland Mail Centre, Auckland 1142, New Zealand. elaine.burgess@plantandfood.co.nz.
authors.forename: Elaine J
authors.initials: EJ
authors.lastname: Burgess
authors.affiliation: Nutrigenomics New Zealand, Plant and Food Research Ltd., Private Bag 92169, Auckland Mail Centre, Auckland 1142, New Zealand. nigel.perry@plantandfood.co.nz.
authors.forename: Nigel B
authors.initials: NB
authors.lastname: Perry
authors.affiliation: Food Nutrition & Health, Food and Bio-Based Products, AgResearch Grasslands Research Centre, Palmerston North 4442, New Zealand. nicole.roy@agresearch.co.nz.
authors.forename: Nicole C
authors.initials: NC
authors.lastname: Roy
mesh_terms: D030024:Curcuma / Q000737:chemistry; D003474:Curcumin / Q000494:pharmacology*; D004847:Epithelial Cells / Q000187:drug effects* / Q000378:metabolism; D057809:HEK293 Cells; D006801:Humans; D066298:In Vitro Techniques; D007249:Inflammation / Q000178:diet therapy*; D015212:Inflammatory Bowel Diseases / Q000178:diet therapy* / Q000235:genetics; D016753:Interleukin-10 / Q000378:metabolism; D027701:Organic Cation Transport Proteins / Q000378:metabolism; D010936:Plant Extracts / Q000737:chemistry; D027981:Symporters
chemical_list: D027701:Organic Cation Transport Proteins; D010936:Plant Extracts; C109807:SLC22A4 protein, human; D027981:Symporters; D016753:Interleukin-10; C012596:turmeric extract; D003474:Curcumin