title: Correlation between spermatogenesis disorders and rat testes CYP2E1 mRNA contents under experimental alcoholism or type I diabetes.
abstract: OBJECTIVE
  The aim of the study was to investigate the correlation between spermatogenesis disorders and CYP2E1 mRNA contents in testes of rats with experimental alcoholism or type I diabetes.
  
  
  METHODS
  Two pathological states characterized by CYP2E1 induction were simulated on Wistar male rats: experimental alcoholism and type I diabetes. As controls for each state, equal number of animals (of the same age and weight) were used. Morphological evaluation of rat testes was carried out. The spermatogenic epithelium state was estimated by four points system. CYP2E1 mRNA expression was rated by method of reverse transcriptase polymerase chain reaction. Pearson correlation coefficients were used for describing relationships between variables.
  
  
  RESULTS
  The presence of alcoholism and diabetes-mediated quantitative and qualitative changes in male rat spermatogenic epithelium in comparison with norm has been demonstrated. The increased levels of testes CYP2E1 have been fixed simultaneously. CYP2E1 mRNA content negatively strongly correlated with spermatogenic index value (r=-0.99; P<0.001) and positively strongly correlated with epithelium desquamation occurrence (r=0.99; P<0.001) in testes of rats with chronic alcoholism. The strong correlation between CYP2E1 mRNA content and number of spermatogonia (r=0.99; P<0.001) and "windows" occurrence (r=0.96; P<0.001) has been fixed in diabetic rats testes.
  
  
  CONCLUSIONS
  Present investigation has demonstrated that the testicular failure following chronic ethanol consumption and diabetes type I in male rats accompanied CYP2E1 mRNA over-expression in testes. The correlation between the levels of CYP2E1 mRNA in testes and spermatogenesis disorders allow supposing the involvement of CYP2E1 into the non-specific pathogenetic mechanisms of male infertility under above-mentioned pathologies.
authors.affiliation: General Toxicology Department, SI "Institute of Pharmacology & Toxicology", National Academy of Medical Sciences of Ukraine, Kyiv, Ukraine. Electronic address: anna_shayakhmetova@yahoo.com.
authors.forename: Ganna M
authors.initials: GM
authors.lastname: Shayakhmetova
authors.affiliation: General Toxicology Department, SI "Institute of Pharmacology & Toxicology", National Academy of Medical Sciences of Ukraine, Kyiv, Ukraine.
authors.forename: Larysa B
authors.initials: LB
authors.lastname: Bondarenko
authors.affiliation: General Toxicology Department, SI "Institute of Pharmacology & Toxicology", National Academy of Medical Sciences of Ukraine, Kyiv, Ukraine.
authors.forename: Anatoliy V
authors.initials: AV
authors.lastname: Matvienko
authors.affiliation: General Toxicology Department, SI "Institute of Pharmacology & Toxicology", National Academy of Medical Sciences of Ukraine, Kyiv, Ukraine.
authors.forename: Valentina M
authors.initials: VM
authors.lastname: Kovalenko
mesh_terms: D000437:Alcoholism / Q000503:physiopathology*; D000818:Animals; D019392:Cytochrome P-450 CYP2E1 / Q000235:genetics / Q000378:metabolism*; D003922:Diabetes Mellitus, Type 1 / Q000150:complications*; D004195:Disease Models, Animal; D004790:Enzyme Induction; D007248:Infertility, Male / Q000150:complications / Q000209:etiology* / Q000378:metabolism / Q000473:pathology; D008297:Male; D012333:RNA, Messenger / Q000378:metabolism; D017208:Rats, Wistar; D013091:Spermatogenesis; D013737:Testis / Q000378:metabolism* / Q000473:pathology
chemical_list: D012333:RNA, Messenger; D019392:Cytochrome P-450 CYP2E1
keywords: Alcoholism; CYP2E1 expression; Correlation; Diabetes; Rats; Spermatogenesis