title: Facile method to sequence cyclic peptides/peptoids via one-pot ring-opening/cleavage reaction.
abstract: A facile method for sequence determination of cyclic peptides/peptoids is described. Macrocyclic peptides/peptoids of 3-10 residues were efficiently synthesized through thioether formation. One-pot reaction of thioether-embedded cyclic peptides/peptoids involving cyanogen bromide-mediated ring-opening and cleavage provides linearized molecules, which can be efficiently sequenced by tandem mass spectrometry.
authors.affiliation: Department of Chemistry, Pohang University of Science and Technology (POSTECH) , Pohang 790-784, South Korea.
authors.forename: Kang Ju
authors.initials: KJ
authors.lastname: Lee
authors.forename: Hyun-Suk
authors.initials: HS
authors.lastname: Lim
mesh_terms: D000595:Amino Acid Sequence; D003488:Cyanogen Bromide / Q000737:chemistry*; D015394:Molecular Structure; D010455:Peptides / Q000138:chemical synthesis* / Q000737:chemistry*; D010456:Peptides, Cyclic / Q000138:chemical synthesis* / Q000737:chemistry*; D013440:Sulfides / Q000737:chemistry*; D053719:Tandem Mass Spectrometry
chemical_list: D010455:Peptides; D010456:Peptides, Cyclic; D013440:Sulfides; D003488:Cyanogen Bromide