title: A molecular fluorescent probe for targeted visualization of temperature at the endoplasmic reticulum.
abstract: The dynamics of cellular heat production and propagation remains elusive at a subcellular level. Here we report the first small molecule fluorescent thermometer selectively targeting the endoplasmic reticulum (ER thermo yellow), with the highest sensitivity reported so far (3.9%/°C). Unlike nanoparticle thermometers, ER thermo yellow stains the target organelle evenly without the commonly encountered problem of aggregation, and successfully demonstrates the ability to monitor intracellular temperature gradients generated by external heat sources in various cell types. We further confirm the ability of ER thermo yellow to monitor heat production by intracellular Ca(2+) changes in HeLa cells. Our thermometer anchored at nearly-zero distance from the ER, i.e. the heat source, allowed the detection of the heat as it readily dissipated, and revealed the dynamics of heat production in real time at a subcellular level.
authors.affiliation: 1] Department of Chemistry, National University of Singapore, MedChem Program of Life Sciences Institute, National University of Singapore, 3 Science Drive 3, Singapore 117543, Republic of Singapore [2] Consolidated Research Institute for Advanced Science and Medical Care, Waseda University, 513 Wasedatsurumaki-cho, Tokyo 162-0041, Japan.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Arai
authors.affiliation: Laboratory of Bioimaging Probe Development, Singapore Bioimaging Consortium, Agency for Science, Technology and Research (A*STAR), Biopolis, Singapore 138667, Republic of Singapore.
authors.forename: Sung-Chan
authors.initials: SC
authors.lastname: Lee
authors.affiliation: Department of Chemistry, National University of Singapore, MedChem Program of Life Sciences Institute, National University of Singapore, 3 Science Drive 3, Singapore 117543, Republic of Singapore.
authors.forename: Duanting
authors.initials: D
authors.lastname: Zhai
authors.affiliation: 1] WASEDA Bioscience Research Institute in Singapore (WABIOS), 11 Biopolis Way, #05-02, Helios, Singapore 138667, Republic of Singapore [2] Organization for University Research Initiatives, Waseda University, #304, Block 120-4, 513 Wasedatsurumaki-cho, Shinjuku-ku, Tokyo, 162-0041, Japan.
authors.forename: Madoka
authors.initials: M
authors.lastname: Suzuki
authors.affiliation: 1] Department of Chemistry, National University of Singapore, MedChem Program of Life Sciences Institute, National University of Singapore, 3 Science Drive 3, Singapore 117543, Republic of Singapore [2] Laboratory of Bioimaging Probe Development, Singapore Bioimaging Consortium, Agency for Science, Technology and Research (A*STAR), Biopolis, Singapore 138667, Republic of Singapore.
authors.forename: Young Tae
authors.initials: YT
authors.lastname: Chang
mesh_terms: D003593:Cytoplasm / Q000378:metabolism; D004721:Endoplasmic Reticulum / Q000378:metabolism*; D005456:Fluorescent Dyes / Q000737:chemistry / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D053758:Nanoparticles / Q000737:chemistry; D013696:Temperature; D013821:Thermometers
chemical_list: D005456:Fluorescent Dyes