title: Expression and cellular distribution of zona pellucida glycoproteins in canine oocytes before and after in vitro maturation.
abstract: This study was aimed at investigating zona pellucida glycoproteins (ZP) ZP2, ZP3 mRNA expression as well as ZP3, ZP4 (ZPB) protein distribution before and after in vitro maturation (IVM) in canine oocytes. The cumulus-oocyte complexes (COCs) were recovered from 27 anoestrous mongrel bitches and matured for 72 h in TCM199 medium. The canine COCs were analysed before and after IVM. Using real-time quantitative polymerase chain reaction (RQ-PCR), both groups of oocytes were analysed for detection of ZP2 and ZP3 mRNA profiles as well as using confocal microscopic analysis for observation of ZP3 and ZP4 protein distribution. In post-IVM canine oocytes an increase in transcript content of ZP2 and ZP3 genes as well as a decrease in ZP3 and ZP4 protein levels were observed when compared with pre-IVM oocytes. Moreover, the ZP4 protein before IVM was significantly distributed in the peripheral area of cytoplasm, whereas after IVM it was localized rather than in the entire cytoplasm. In contrast, the ZP3 protein was found both before and after IVM was distributed in the peripheral area of the cytoplasm. In conclusion, we suggest that the expression of ZP2 and ZP3 genes is associated with the maturation stage of canine oocytes, as higher mRNAs levels were found after IVM. However, a decreased expression of ZP3 and ZP4 proteins after IVM suggests maturation-dependent down-regulation of these protein translations, which may result in disturbed fertilization.
authors.affiliation: Department of Histology and Embryology,Department of Anatomy,Poznan University of Medical Sciences,6 Święcickiego St.,60-781 Poznań,Poland.
authors.forename: Bartosz
authors.initials: B
authors.lastname: Kempisty
authors.affiliation: Department of Toxicology,Poznan University of Medical Sciences,Poznan,Poland.
authors.forename: Hanna
authors.initials: H
authors.lastname: Piotrowska
authors.affiliation: Institute of Veterinary Sciences,Poznan University of Life Sciences,Poznan,Poland.
authors.forename: Dorota
authors.initials: D
authors.lastname: Bukowska
authors.affiliation: Institute of Veterinary Sciences,Poznan University of Life Sciences,Poznan,Poland.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Woźna
authors.affiliation: Department of Histology and Embryology,Poznan University of Medical Sciences,Poznan,Poland.
authors.forename: Sylwia
authors.initials: S
authors.lastname: Ciesiółka
authors.affiliation: Institute of Veterinary Sciences,Poznan University of Life Sciences,Poznan,Poland.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Wojtanowicz-Markiewicz
authors.affiliation: Institute of Veterinary Sciences,Poznan University of Life Sciences,Poznan,Poland.
authors.forename: Renata
authors.initials: R
authors.lastname: Włodarczyk
authors.affiliation: Department of Histology and Embryology,Poznan University of Medical Sciences,Poznan,Poland.
authors.forename: Karol
authors.initials: K
authors.lastname: Jopek
authors.affiliation: Department of Genetics and Reproduction,Veterinary Research Institute,Brno,Czech Republic.
authors.forename: Michal
authors.initials: M
authors.lastname: Jeseta
authors.affiliation: Department of Anatomy,Poznan University of Medical Sciences,Poznan,Poland.
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Bruska
authors.affiliation: Department of Histology and Embryology,Poznan University of Medical Sciences,Poznan,Poland.
authors.forename: Michał
authors.initials: M
authors.lastname: Nowicki
authors.affiliation: Institute of Veterinary Sciences,Poznan University of Life Sciences,Poznan,Poland.
authors.forename: Jędrzej M
authors.initials: JM
authors.lastname: Jaśkowski
authors.affiliation: Institute of Reproductive Biology,Leibniz Institute for Farm Animal Biology,Wilhelm-Stahl-Allee 2,18196 Dummerstorf,Germany.
authors.forename: Klaus-Peter
authors.initials: KP
authors.lastname: Brüssow
authors.affiliation: Department of Histology and Embryology,Wroclaw Medical University,Wroclaw,Poland.
authors.forename: Maciej
authors.initials: M
authors.lastname: Zabel
mesh_terms: D000818:Animals; D054885:Cumulus Cells / Q000166:cytology / Q000502:physiology; D004285:Dogs; D004527:Egg Proteins / Q000235:genetics* / Q000378:metabolism*; D005260:Female; D005786:Gene Expression Regulation; D059471:In Vitro Oocyte Maturation Techniques / Q000379:methods*; D008562:Membrane Glycoproteins / Q000235:genetics* / Q000378:metabolism*; D018613:Microscopy, Confocal / Q000379:methods; D009865:Oocytes / Q000502:physiology*; D011956:Receptors, Cell Surface / Q000235:genetics* / Q000378:metabolism*; D000072216:Zona Pellucida Glycoproteins
chemical_list: D004527:Egg Proteins; D008562:Membrane Glycoproteins; D011956:Receptors, Cell Surface; D000072216:Zona Pellucida Glycoproteins
keywords: Bitch; Glycoproteins; Oocyte; Subcellular protein distribution; Zona pellucida