title: Flow cytometric assessment of morphology, viability, and production of reactive oxygen species of Crassostrea gigas oocytes. Application to toxic dinoflagellate (Alexandrium minutum) exposure.
abstract: The Pacific oyster Crassostrea gigas accounts for a large part of shellfish aquaculture production worldwide. Aspects of morphological and functional characteristics of oyster oocytes remain poorly documented, and traditional techniques, such as microscopic observations of shape or fertilization rate, are time and space consuming. The purpose of this study was to assess for the first time viability and reactive oxygen species (ROS) production of Pacific oyster oocytes using flow cytometry (FCM) and to apply this method to determine oocyte responses to in vitro exposure to the toxic dinoflagellate Alexandrium minutum. A culture of A. minutum caused a significant increase in oocyte ROS production, which gradually increased with the age of the culture, but viability was not affected. Effect of the supernatant of the same A. minutum culture did not cause any significant modifications of oocyte morphology, viability, or ROS level. This study confirmed that some oocyte cellular characteristics can be assessed using FCM techniques.
authors.affiliation: Laboratoire des Sciences de l'Environnement MARin (LEMAR), IUEM, Technopôle Brest Iroise, 29820, Plouzané, France.
authors.forename: Nelly
authors.initials: N
authors.lastname: Le Goïc
authors.forename: Hélène
authors.initials: H
authors.lastname: Hégaret
authors.forename: Myrina
authors.initials: M
authors.lastname: Boulais
authors.forename: Jean-Philippe
authors.initials: JP
authors.lastname: Béguel
authors.forename: Christophe
authors.initials: C
authors.lastname: Lambert
authors.forename: Caroline
authors.initials: C
authors.lastname: Fabioux
authors.forename: Philippe
authors.initials: P
authors.lastname: Soudant
mesh_terms: D000818:Animals; D002470:Cell Survival; D049896:Crassostrea / Q000469:parasitology*; D004141:Dinoflagellida; D005260:Female; D005434:Flow Cytometry / Q000379:methods*; D009865:Oocytes / Q000469:parasitology*; D011529:Protozoan Infections, Animal / Q000175:diagnosis*; D017382:Reactive Oxygen Species / Q000032:analysis*
chemical_list: D017382:Reactive Oxygen Species
keywords: Pacific oyster; ROS production; ecotoxicology; flow cytometry; gamete quality; viability