title: Lack of human papillomavirus DNA in colon adenocarcinama and adenoma.
abstract: BACKGROUND
  Human papilloma viruses (HPV) have been detected in several types of cancers. Over the last few years, a possible correlation between HPV infection and colon cancers has been suggested. The aim of this study was to assess the presence of HPV-DNA in colon adenocarcinomas and adenomas to describe the relationship of HPV infection with these pathologic features.
  
  
  METHODS
  The study included formalin-fixed and paraffin-embedded tissue samples of 70 patients with colon adenocarcinoma and 30 patients with adenoma (as study group) and 30 tumor adjacent tissues (as control). HPV-DNA was purified and first amplified through MY09/MY11 and GP5+/GP6+ primers. Subsequently, for more precision, another PCR was performed using PGMY09/11 L1 consensus primer system.
  
  
  RESULTS
  All tested adenocarcinomas and adenomas as well as normal tumor adjacent tissues were negative for all types of HPV in two PCR assays.
  
  
  CONCLUSIONS
  Our results do not support the relationship between HPV infection and colon carcinoma or adenoma. Attributing a role to the HPV in the etiology of colon carcinogenesis will require further studies.
authors.forename: Arezoo
authors.initials: A
authors.lastname: Aghakhani
authors.forename: Rasool
authors.initials: R
authors.lastname: Hamkar
authors.affiliation: Department of Clinical Research, Pasteur Institute of Iran, Tehran, Iran.
authors.forename: Amitis
authors.initials: A
authors.lastname: Ramezani
authors.forename: Farahnaz
authors.initials: F
authors.lastname: Bidari-Zerehpoosh
authors.forename: Shahram
authors.initials: S
authors.lastname: Sabeti
authors.forename: Nastaran
authors.initials: N
authors.lastname: Ghavami
authors.forename: Mohammad
authors.initials: M
authors.lastname: Banifazl
authors.forename: Niloofar
authors.initials: N
authors.lastname: Rashidi
authors.forename: Ali
authors.initials: A
authors.lastname: Eslamifar
mesh_terms: D000230:Adenocarcinoma / Q000209:etiology* / Q000473:pathology / Q000821:virology; D000236:Adenoma / Q000209:etiology* / Q000473:pathology / Q000821:virology; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D052685:Alphapapillomavirus / Q000235:genetics*; D003110:Colonic Neoplasms / Q000209:etiology* / Q000473:pathology / Q000821:virology; D004279:DNA, Viral; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D060787:Neoplasm Grading; D030361:Papillomavirus Infections / Q000150:complications* / Q000821:virology; D016133:Polymerase Chain Reaction; D014412:Tumor Virus Infections / Q000150:complications* / Q000821:virology
chemical_list: D004279:DNA, Viral