title: Tumor cell lysate induces the immunosuppression and apoptosis of mouse immunocytes.
abstract: Although tumor cell lysate (TCL) is a type of immunocyte stimulator, its immunosuppressive function must not be ignored. The present study reported that TCL prepared from a Lewis lung cancer cell was able to induce the development of immunosuppressive macrophages (MΦ) and tolerogenic dendritic cells. In addition, TCL upregulated the expression of CD69 in mouse splenocytes, and cell apoptosis and the percentage of regulatory T cells in mouse splenocytes simultaneously increased. Furthermore, the present study found that the immunosuppressive factor, hyaluronan, and the apoptosis inducers, Fas ligand and transforming growth factor-β, are present in TCL. These components may be associated with the emergence of immunosuppressive cells or splenocyte apoptosis. Thus, the present study has enriched our understanding of the composition of TCL and its negative regulatory effect on immunocytes.
authors.affiliation: Department of Biochemistry, Wannan Medical College, Wuhu, Anhui 241002, P.R. China.
authors.forename: Bohan
authors.initials: B
authors.lastname: Dong
authors.affiliation: Department of Gynaecology and Obstetrics, Traditional Chinese Medical Hospital of Wuhu, Wuhu, Anhui 241000, P.R. China.
authors.forename: Guangli
authors.initials: G
authors.lastname: Dai
authors.affiliation: Department of Biochemistry, Wannan Medical College, Wuhu, Anhui 241002, P.R. China.
authors.forename: Lei
authors.initials: L
authors.lastname: Xu
authors.affiliation: Department of Biochemistry, Wannan Medical College, Wuhu, Anhui 241002, P.R. China.
authors.forename: Yao
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Biochemistry, Wannan Medical College, Wuhu, Anhui 241002, P.R. China.
authors.forename: Liefeng
authors.initials: L
authors.lastname: Ling
authors.affiliation: Department of Biochemistry, Wannan Medical College, Wuhu, Anhui 241002, P.R. China.
authors.forename: Lingling
authors.initials: L
authors.lastname: Sun
authors.affiliation: Department of Biochemistry, Wannan Medical College, Wuhu, Anhui 241002, P.R. China.
authors.forename: Jun
authors.initials: J
authors.lastname: Lv
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000276:immunology*; D045744:Cell Line, Tumor; D003713:Dendritic Cells / Q000276:immunology*; D053222:Fas Ligand Protein / Q000276:immunology; D005260:Female; D006820:Hyaluronic Acid / Q000276:immunology; D007108:Immune Tolerance / Q000276:immunology*; D007165:Immunosuppression Therapy / Q000379:methods; D008175:Lung Neoplasms / Q000276:immunology*; D008264:Macrophages / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D013154:Spleen / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology; D016212:Transforming Growth Factor beta / Q000276:immunology
chemical_list: D053222:Fas Ligand Protein; D016212:Transforming Growth Factor beta; D006820:Hyaluronic Acid