title: Detection of antibodies against Anaplasma phagocytophilum in dogs using an automated fluorescence based system.
abstract: Infection with Anaplasma phagocytophilum can cause significant illness in some dogs and accurate diagnostic assays are needed. The objectives of the study were to optimize an automated fluorescence system for the detection of antibodies against A. phagocytophilum in canine serum. Serum and blood was collected temporally from seven dogs inoculated parenterally with culture-derived A. phagocytophilum and from 36 dogs exposed to wild-caught, adult Ixodes scapularis for 7 days. The system was optimized using the samples from the parenterally inoculated dogs. The ability to detect antibodies against A. phagocytophilum in the I. scapularis exposed dogs by the automated system was compared with a diagnostic kit (ELISA) and an indirect fluorescent antibody assay (IFA). Each blood sample was also assayed for A. phagocytophilum DNA by polymerase chain reaction (PCR). Of the 36 dogs exposed to I. scapularis, A. phagocytophilum DNA was amplified from blood from 22 dogs by PCR with first positive results occurring on weeks 1 (seven dogs), 2 (nine dogs), 3 (four dogs), 4 (one dog), or 5 (one dog). PCR results were positive prior to detection of antibodies in any of the three antibody assays for 19 dogs. The automated fluorescence system and IFA detected antibodies against A. phagocytophilum earlier than the ELISA. In conclusion, A. phagocytophilum PCR assays on blood are indicated in dogs with suspected acute anaplasmosis if serum antibody assays are negative.
authors.affiliation: Antech Laboratories, Lake Success, NY, USA.
authors.forename: Scott
authors.initials: S
authors.lastname: Moroff
authors.affiliation: Antech Laboratories, Lake Success, NY, USA.
authors.forename: Irene
authors.initials: I
authors.lastname: Sokolchik
authors.affiliation: Antech Laboratories, Lake Success, NY, USA.
authors.forename: Todd
authors.initials: T
authors.lastname: Woodring
authors.affiliation: Antech Laboratories, Lake Success, NY, USA.
authors.forename: Craig
authors.initials: C
authors.lastname: Woodruff
authors.affiliation: Antech Laboratories, Lake Success, NY, USA.
authors.forename: Bret
authors.initials: B
authors.lastname: Atkinson
authors.affiliation: Center for Companion Animal Studies at Colorado State University, Fort Collins, CO, USA. Electronic address: mlappin@colostate.edu.
authors.forename: Michael R
authors.initials: MR
authors.lastname: Lappin
mesh_terms: D041081:Anaplasma phagocytophilum / Q000235:genetics / Q000302:isolation & purification*; D000712:Anaplasmosis / Q000175:diagnosis* / Q000469:parasitology; D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood; D004283:Dog Diseases / Q000175:diagnosis* / Q000469:parasitology; D004285:Dogs; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary*; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect / Q000662:veterinary*; D018884:Ixodes / Q000469:parasitology; D008297:Male; D009154:Mutation; D016133:Polymerase Chain Reaction / Q000662:veterinary; D015800:Protozoan Proteins / Q000235:genetics
chemical_list: D000913:Antibodies, Protozoan; D015800:Protozoan Proteins
keywords: Anaplasma; Diagnostic; Dog; PCR; Serology