title: The effects of nesfatin-1 in the paraventricular nucleus on gastric motility and its potential regulation by the lateral hypothalamic area in rats.
abstract: The current study investigated the effects of nesfatin-1 in the hypothalamic paraventricular nucleus (PVN) on gastric motility and the regulation of the lateral hypothalamic area (LHA). Using single unit recordings in the PVN, we show that nesfatin-1 inhibited the majority of the gastric distention (GD)-excitatory neurons and excited more than half of the GD-inhibitory (GD-I) neurons in the PVN, which were weakened by oxytocin receptor antagonist H4928. Gastric motility experiments showed that administration of nesfatin-1 in the PVN decreased gastric motility, which was also partly prevented by H4928. The nesfatin-1 concentration producing a half-maximal response (EC50) in the PVN was lower than the value in the dorsomedial hypothalamic nucleus, while nesfatin-1 in the reuniens thalamic nucleus had no effect on gastric motility. Retrograde tracing and immunofluorescent staining showed that nucleobindin-2/nesfatin-1 and fluorogold double-labeled neurons were observed in the LHA. Electrical LHA stimulation changed the firing rate of GD-responsive neurons in the PVN. Pre-administration of an anti- nucleobindin-2/nesfatin-1 antibody in the PVN strengthened gastric motility and decreased the discharging of the GD-I neurons induced by electrical stimulation of the LHA. These results demonstrate that nesfatin-1 in the PVN could serve as an inhibitory factor to inhibit gastric motility, which might be regulated by the LHA.
authors.affiliation: Department of Pathophysiology, Medical College of Qingdao University, Qingdao, China.
authors.forename: Fei-fei
authors.initials: FF
authors.lastname: Guo
authors.forename: Luo
authors.initials: L
authors.lastname: Xu
authors.forename: Sheng-li
authors.initials: SL
authors.lastname: Gao
authors.forename: Xiang-rong
authors.initials: XR
authors.lastname: Sun
authors.forename: Zhi-ling
authors.initials: ZL
authors.lastname: Li
authors.forename: Yan-ling
authors.initials: YL
authors.lastname: Gong
mesh_terms: D000818:Animals; D002135:Calcium-Binding Proteins / Q000494:pharmacology*; D004268:DNA-Binding Proteins / Q000494:pharmacology*; D004558:Electric Stimulation; D005746:Gastric Emptying / Q000187:drug effects; D005769:Gastrointestinal Motility / Q000187:drug effects*; D007026:Hypothalamic Area, Lateral / Q000187:drug effects*; D008297:Male; D009419:Nerve Tissue Proteins / Q000494:pharmacology*; D009474:Neurons / Q000187:drug effects; D000080866:Nucleobindins; D010286:Paraventricular Hypothalamic Nucleus / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar; D018045:Receptors, Oxytocin / Q000037:antagonists & inhibitors; D013270:Stomach / Q000187:drug effects*
chemical_list: D002135:Calcium-Binding Proteins; D004268:DNA-Binding Proteins; D009419:Nerve Tissue Proteins; C000630652:Nucb1 protein, rat; D000080866:Nucleobindins; D018045:Receptors, Oxytocin
keywords: gastric distension responsive neurons; gastric motility; lateral hypothalamic area; nesfatin-1; paraventricular nucleus