title: Application of diffusion ordered-1H-nuclear magnetic resonance spectroscopy to quantify sucrose in beverages.
abstract: This work focuses on a quantitative analysis of sucrose using diffusion ordered-quantitative (1)H-nuclear magnetic resonance spectroscopy (DOSY-qNMR), where an analyte can be isolated from interference based on its characteristic diffusion coefficient (D) in gradient magnetic fields. The D value of sucrose in deuterium oxide at 30°C was 4.9 × 10(-10)m(2)/s at field gradient pulse from 5.0 × 10(-2) to 3.0 × 10(-1)T/m, separated from other carbohydrates (glucose and fructose). Good linearity (r(2)=0.9999) was obtained between sucrose (0.5-20.0 g/L) and the resonance area of target glucopyranosyl-α-C1 proton normalised to that of cellobiose C1 proton (100.0 g/L, as an internal standard) in 1D sliced DOSY spectrum. The DOSY-qNMR method was successfully applied to quantify sucrose in orange juice (36.1 ± 0.5 g/L), pineapple juice (53.5 ± 1.1g/L) and a sports drink (24.7 ± 0.6g/L), in good agreement with the results obtained by an F-kit method.
authors.affiliation: Division of Bioresources and Biosciences, Faculty of Agriculture, Graduate School of Kyushu University, 6-10-1 Hakozaki, Fukuoka 812-8581, Japan.
authors.forename: Ruge
authors.initials: R
authors.lastname: Cao
authors.affiliation: Division of Bioresources and Biosciences, Faculty of Agriculture, Graduate School of Kyushu University, 6-10-1 Hakozaki, Fukuoka 812-8581, Japan.
authors.forename: Airi
authors.initials: A
authors.lastname: Nonaka
authors.affiliation: Division of Bioresources and Biosciences, Faculty of Agriculture, Graduate School of Kyushu University, 6-10-1 Hakozaki, Fukuoka 812-8581, Japan.
authors.forename: Fusae
authors.initials: F
authors.lastname: Komura
authors.affiliation: Division of Bioresources and Biosciences, Faculty of Agriculture, Graduate School of Kyushu University, 6-10-1 Hakozaki, Fukuoka 812-8581, Japan. Electronic address: tmatsui@agr.kyushu-u.ac.jp.
authors.forename: Toshiro
authors.initials: T
authors.lastname: Matsui
mesh_terms: D002475:Cellobiose / Q000032:analysis; D004058:Diffusion; D061215:Energy Drinks / Q000032:analysis* / Q000592:standards; D005504:Food Analysis / Q000295:instrumentation / Q000379:methods*; D005632:Fructose / Q000032:analysis; D000067030:Fruit and Vegetable Juices / Q000032:analysis* / Q000592:standards; D005947:Glucose / Q000032:analysis; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D011522:Protons; D013395:Sucrose / Q000032:analysis*
chemical_list: D011522:Protons; D002475:Cellobiose; D005632:Fructose; D013395:Sucrose; D005947:Glucose
keywords: DOSY; Food analysis; Sucrose; qNMR