title: Pullulan/dextran/nHA macroporous composite beads for bone repair in a femoral condyle defect in rats.
abstract: The repair of bone defects is of particular interest for orthopedic, oral, maxillofacial, and dental surgery. Bone loss requiring reconstruction is conventionally addressed through bone grafting. Depending on the size and the location of the defect, this method has limits and risks. Biomaterials can offer an alternative and have features supporting bone repair. Here, we propose to evaluate the cellular penetration and bone formation of new macroporous beads based on pullulan/dextran that has been supplemented with nanocrystalline hydroxyapatite in a rat model. Cross-linked beads of 300-500 µm diameters were used in a lateral femoral condyle defect and analyzed by magnetic resonance imaging, micro-computed tomography, and histology in comparison to the empty defects 15, 30, and 70 days after implantation. Inflammation was absent for both conditions. For empty defects, cellularisation and mineralization started from the periphery of the defect. For the defects containing beads, cellular structures filling out the spaces between the scaffolds with increasing interconnectivity and trabecular-like organization were observed over time. The analysis of calcified sections showed increased mineralization over time for both conditions, but was more pronounced for the samples containing beads. Bone Mineral Density and Bone Mineral Content were both significantly higher at day 70 for the beads in comparison to empty defects as well as compared with earlier time points. Analysis of newly formed tissue around the beads showed an increase of osteoid tissue, measured as percentage of the defect surface. This study suggests that the use of beads for the repair of small size defects in bone may be expanded on to meet the clinical need for a ready-to-use fill-up material that can favor bone formation and mineralization, as well as promote vessel ingrowth into the defect site.
authors.affiliation: CIC 1401, University hospital of Bordeaux/Inserm, Bordeaux, France.
authors.forename: Silke
authors.initials: S
authors.lastname: Schlaubitz
authors.affiliation: U1148, LVTS/Inserm, Paris, France; Près Sorbonne Paris Cité, University of Paris Nord and University Paris Diderot, Paris, France.
authors.forename: Sidi Mohammed
authors.initials: SM
authors.lastname: Derkaoui
authors.affiliation: U1026 Tissue Bioengineering, University of Bordeaux/Inserm, Bordeaux, France.
authors.forename: Lydia
authors.initials: L
authors.lastname: Marosa
authors.affiliation: RMSB Center/UMR 5536, CNRS, Bordeaux, France.
authors.forename: Sylvain
authors.initials: S
authors.lastname: Miraux
authors.affiliation: CIC 1401, University hospital of Bordeaux/Inserm, Bordeaux, France.
authors.forename: Martine
authors.initials: M
authors.lastname: Renard
authors.affiliation: U1026 Tissue Bioengineering, University of Bordeaux/Inserm, Bordeaux, France; Dental School, University of Bordeaux, Bordeaux, France.
authors.forename: Sylvain
authors.initials: S
authors.lastname: Catros
authors.affiliation: U1148, LVTS/Inserm, Paris, France; Près Sorbonne Paris Cité, University of Paris Nord and University Paris Diderot, Paris, France.
authors.forename: Catherine
authors.initials: C
authors.lastname: Le Visage
authors.affiliation: U1148, LVTS/Inserm, Paris, France; Près Sorbonne Paris Cité, University of Paris Nord and University Paris Diderot, Paris, France.
authors.forename: Didier
authors.initials: D
authors.lastname: Letourneur
authors.affiliation: U1026 Tissue Bioengineering, University of Bordeaux/Inserm, Bordeaux, France.
authors.forename: Joëlle
authors.initials: J
authors.lastname: Amédée
authors.affiliation: U1026 Tissue Bioengineering, University of Bordeaux/Inserm, Bordeaux, France; Dental School, University of Bordeaux, Bordeaux, France.
authors.forename: Jean-Christophe
authors.initials: JC
authors.lastname: Fricain
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry / Q000494:pharmacology*; D001861:Bone Regeneration / Q000187:drug effects; D002113:Calcification, Physiologic / Q000187:drug effects; D003911:Dextrans / Q000737:chemistry*; D017886:Durapatite / Q000737:chemistry*; D005269:Femur / Q000000981:diagnostic imaging / Q000187:drug effects / Q000502:physiology*; D005936:Glucans / Q000737:chemistry*; D008297:Male; D008863:Microspheres; D053758:Nanoparticles; D016062:Porosity; D011859:Radiography; D051381:Rats; D023822:Tissue Engineering; D054457:Tissue Scaffolds
chemical_list: D001672:Biocompatible Materials; D003911:Dextrans; D005936:Glucans; C009109:pullulan; D017886:Durapatite