title: Sequence and chiral selectivity of drug-DNA interactions revealed by force spectroscopy.
abstract: Differential binding force has been used to precisely characterize the mechanical effect of a drug molecule binding to a DNA duplex. The high-resolution binding forces measured by the force-induced remnant magnetization spectroscopy (FIRMS) enable the binding behavior of drug molecules with different chirality and DNA of various sequences to be distinguished. The sequence specificity of Hg(2+) and daunomycin was revealed by force spectroscopy for the first time, and the results are consistent with those obtained by other techniques. Furthermore, the two isomers of d,l-tetrahydropalmatine showed selectivity for two different DNA sequences. One particular useful feature of this approach is that the small molecules under study do not require any labels.
authors.affiliation: Department of Chemistry, University of Houston, Houston, TX 77204 (USA) http://www.chem.uh.edu/Faculty/Xu/
authors.forename: Qiongzheng
authors.initials: Q
authors.lastname: Hu
authors.forename: Shoujun
authors.initials: S
authors.lastname: Xu
mesh_terms: D001483:Base Sequence; D001600:Berberine Alkaloids / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D003630:Daunorubicin / Q000737:chemistry*; D008628:Mercury / Q000737:chemistry*; D018625:Microscopy, Atomic Force; D013237:Stereoisomerism
chemical_list: D001600:Berberine Alkaloids; C014215:tetrahydropalmatine; D004247:DNA; D008628:Mercury; D003630:Daunorubicin
keywords: DNA recognition; chirality; drug screening; force spectroscopy; noncovalent interactions