title: The forward and backward transport processes in the AOT/hexane reversed micellar extraction of soybean protein.
abstract: Soybean protein was taken as a model protein to investigate two aspects of the protein extraction by sodium bis(2-ethylhexyl) sulfosuccinate (AOT) reverse micelles: (1) the forward protein extraction from the solid state, and the effect of pH, AOT concentration, alcohol and water content (W0) on the transfer efficiency; (2) the back-transfer, the capability of the protein to be recovered from the micellar solution. The experimental results led to the conclusion that the highest forward extraction efficiency of soybean protein was reached at AOT concentration 180 mmol l(-1), aqueous pH 7.0, KCl concentration 0.05 mol l(-1), 0.5 % (v/v) alcohol, W0 18. Under these conditions, the forward extraction efficiency of soybean protein achieved 70.1 %. It was noted that the percentage of protein back extraction depended on the salt concentration and pH value. Around 92 % of protein recovery was obtained after back extraction.
authors.affiliation: Institute Agro-Food Science and Technology, Shandong Academy of Agricultural Sciences, Jinan, 250100 China.
authors.forename: Jun
authors.initials: J
authors.lastname: Chen
authors.affiliation: Institute Agro-Food Science and Technology, Shandong Academy of Agricultural Sciences, Jinan, 250100 China.
authors.forename: Fengliang
authors.initials: F
authors.lastname: Chen
authors.affiliation: Institute Agro-Food Science and Technology, Shandong Academy of Agricultural Sciences, Jinan, 250100 China.
authors.forename: Xianchang
authors.initials: X
authors.lastname: Wang
authors.affiliation: Institute Agro-Food Science and Technology, Shandong Academy of Agricultural Sciences, Jinan, 250100 China ; Institute Agro-Food Science and Technology, Shandong Academy of Agricultural Sciences, No. 198, Gongyebei Road, Jinan, 250100 China.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Zhao
authors.affiliation: Institute of Neurological Disorders, Tsinghua University, Beijing, 100049 People's Republic of China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Ao
keywords: Backward extraction; Forward extraction; Reverse micelles; Soybean protein