title: Inhibitory effect of b-AP15 on the 20S proteasome.
abstract: The 26S proteasome is a cellular proteolytic complex containing 19S regulatory particles and the 20S core proteasome. It was reported that the small molecule b-AP15 targets the proteasome by inhibiting deubiquitination of the 19S regulatory particles of the proteasome complex. An investigation of b-AP15 on the 20S proteasome core suggested that this compound can also inhibit the 20S proteasome with a potency equivalent to that found to inhibit the 19S regulatory particles.
authors.affiliation: Department of Surgery, Duke University Medical Center, Durham, NC 27710, USA. li.huang@duke.edu.
authors.forename: Li
authors.initials: L
authors.lastname: Huang
authors.affiliation: Department of Surgery, Duke University Medical Center, Durham, NC 27710, USA. kathy.jung@duke.edu.
authors.forename: Katherine
authors.initials: K
authors.lastname: Jung
authors.affiliation: Department of Surgery, Duke University Medical Center, Durham, NC 27710, USA. chc@duke.edu.
authors.forename: Chin Ho
authors.initials: CH
authors.lastname: Chen
mesh_terms: D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D006367:HeLa Cells / Q000187:drug effects; D006801:Humans; D020128:Inhibitory Concentration 50; D010881:Piperidones / Q000737:chemistry / Q000494:pharmacology*; D011480:Protease Inhibitors / Q000494:pharmacology*; D046988:Proteasome Endopeptidase Complex / Q000378:metabolism*; D025801:Ubiquitin / Q000235:genetics / Q000378:metabolism
chemical_list: C569633:3,5-bis((4-nitrophenyl)methylidene)-1-prop-2-enoylpiperidin-4-one; D010881:Piperidones; D011480:Protease Inhibitors; D025801:Ubiquitin; D049452:Green Fluorescent Proteins; D046988:Proteasome Endopeptidase Complex; C119011:26S proteasome non-ATPase regulatory subunit 13