title: Delivery of siRNAs to cancer cells via bacteria.
abstract: RNA interference (RNAi) technology is a promising approach for efficient silencing of a particular gene for cancer gene therapy. However, the main obstacle for the development of RNAi-based therapeutic approaches is the delivery of the RNAi effector molecules to target cells. One promising strategy to surmount this challenge is the application of nonpathogenic bacteria as a delivery vector to target cells. In this chapter, the design of invasive Escherichia coli is described. The strain carries a plasmid encoding short hairpin RNAs (shRNAs), a protein (invasin) necessary for endocytotic absorption of the bacteria by target cells, and listeriolysin O required for the lysis of endocytotic vesicles within the target cells.
authors.affiliation: Charité Campus Mitte, Institute of Pathology, Charitéplatz 1, Berlin, 10117, Germany.
authors.forename: Omar
authors.initials: O
authors.lastname: Ahmed
authors.forename: Andrea
authors.initials: A
authors.lastname: Krühn
authors.forename: Hermann
authors.initials: H
authors.lastname: Lage
mesh_terms: D000165:Acridine Orange; D018829:Adhesins, Bacterial / Q000235:genetics / Q000378:metabolism; D001427:Bacterial Toxins / Q000235:genetics / Q000378:metabolism; D001692:Biological Transport; D045744:Cell Line, Tumor; D016503:Drug Delivery Systems / Q000379:methods*; D004705:Endocytosis; D004847:Epithelial Cells / Q000378:metabolism / Q000382:microbiology / Q000473:pathology; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D005456:Fluorescent Dyes; D005753:Gastric Mucosa / Q000378:metabolism / Q000382:microbiology / Q000473:pathology; D015870:Gene Expression; D006360:Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism; D006460:Hemolysin Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D007211:Indoles; D008856:Microscopy, Fluorescence; D010957:Plasmids / Q000737:chemistry / Q000378:metabolism*; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D022161:Transport Vesicles / Q000378:metabolism* / Q000382:microbiology
chemical_list: D018829:Adhesins, Bacterial; D001427:Bacterial Toxins; D005456:Fluorescent Dyes; D006360:Heat-Shock Proteins; D006460:Hemolysin Proteins; D007211:Indoles; D034741:RNA, Small Interfering; C053476:invasin, Yersinia; C007293:DAPI; D000165:Acridine Orange; C038705:hlyA protein, Listeria monocytogenes