title: Downregulation of receptor tyrosine kinases through ubiquitination: analysis by immunodetection.
abstract: After ligand binding, receptor tyrosine kinases (RTKs) transmit intracellular signals involved in the regulation of various cell events and then attenuate signal transduction. Ubiquitination is a critical step involved in the downregulation of RTK signaling. Here, we describe how to immunodetect the ligand-induced ubiquitination and degradation of TrkA, an RTK, by immunoprecipitation and Western blotting.
authors.affiliation: Department of Integrative Physiology, Gunma University Graduate School of Medicine, Showa-machi, Maebashi, Gunma, 371-8511, Japan, simokawa@med.gunma-u.ac.jp.
authors.forename: Noriaki
authors.initials: N
authors.lastname: Shimokawa
authors.forename: Noriyuki
authors.initials: N
authors.lastname: Koibuchi
mesh_terms: D000906:Antibodies / Q000737:chemistry; D015153:Blotting, Western; D015536:Down-Regulation; D004591:Electrophoresis, Polyacrylamide Gel; D057809:HEK293 Cells; D006801:Humans; D047468:Immunoprecipitation / Q000379:methods*; D008024:Ligands; D020932:Nerve Growth Factor / Q000494:pharmacology; D010957:Plasmids / Q000737:chemistry / Q000378:metabolism; D050721:Proto-Oncogene Proteins c-cbl / Q000235:genetics / Q000378:metabolism*; D020917:Receptor, trkA / Q000235:genetics / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction; D014162:Transfection; D054875:Ubiquitination / Q000187:drug effects
chemical_list: D000906:Antibodies; D008024:Ligands; D011993:Recombinant Fusion Proteins; D020932:Nerve Growth Factor; D050721:Proto-Oncogene Proteins c-cbl; D020917:Receptor, trkA; C067419:CBL protein, human