title: Correlation between E-cadherin-regulated cell adhesion and human osteosarcoma MG-63 cell anoikis.
abstract: OBJECTIVE
  The aim of this study was to investigate the relationship between cell adhesion and anoikis evasion among human osteosarcoma cells (MG-63), and to further study the molecular mechanisms.
  
  
  METHODS
  Human osteosarcoma cells (MG-63) were assessed for apoptosis, and caspase-3, E-cadherin and β-catenin expression in EDTA and control non-EDTA groups.
  
  
  RESULTS
  MG-63 cells were predominantly aggregated when in suspension, and the suspended cells were more dispersed in the EDTA group. Following culture in suspension for 24 h, 48 h, or 72 h, the rates of apoptosis were 34.88%±3.64%, 59.3%±7.22% and 78.5%±5.21% in the experimental group and 7.34%±2.13%, 14.7%±3.69%, and 21.4%±3.60% in the control group, respectively. Caspase-3 expression progressively increased and E-cadherin and β-catenin were decreased in the experimental group, whereas there was no change in the control group.
  
  
  CONCLUSIONS
  MG-63 cells could avoid anoikis through cell adhesion, and E-cadherin might play a role in this process.
authors.affiliation: Department of Orthopedics, the 2nd Affiliated Hospital of Wenzhou Medical University, Key Lab of Zhejiang Orthopedics Science, Wenzhou, China E-mail : dingshenglincn@163.com.
authors.forename: Ding-Sheng
authors.initials: DS
authors.lastname: Lin
authors.forename: Le-Yi
authors.initials: LY
authors.lastname: Cai
authors.forename: Jian
authors.initials: J
authors.lastname: Ding
authors.forename: Wei-Yang
authors.initials: WY
authors.lastname: Gao
mesh_terms: D023102:Anoikis / Q000502:physiology*; D017209:Apoptosis; D015153:Blotting, Western; D001859:Bone Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D015820:Cadherins / Q000235:genetics / Q000378:metabolism*; D053148:Caspase 3 / Q000235:genetics / Q000378:metabolism; D002448:Cell Adhesion / Q000502:physiology*; D049109:Cell Proliferation; D005434:Flow Cytometry; D006801:Humans; D012516:Osteosarcoma / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014407:Tumor Cells, Cultured; D051176:beta Catenin / Q000235:genetics / Q000378:metabolism
chemical_list: D015820:Cadherins; D012333:RNA, Messenger; D051176:beta Catenin; D053148:Caspase 3