title: Characterization and partial genomic analysis of a lytic Myoviridae bacteriophage against Staphylococcus aureus isolated from dairy cows with mastitis in Mid-east of China.
abstract: Using bacteriophages as a tool to the control of pathogens is a complementary to antibiotic therapy. We have isolated a lytic bacteriophage, designated vB_SauM_JS25, from sewage effluent on a dairy farm in Jiangsu, Mid-east of China for use as a biocontrol agent against Staphylococcus aureus infections. Phage vB_SauM_JS25 was morphologically classified as Myoviridae. The phage showed broad host ranges within S. aureus strains, lysing 51 of 56 strains (91.1 %). Its latent period and burst size were approximately 20 min and 21 PFU/cell, respectively. Phage vB_SauM_JS25 was able to survive in a pH range between 6 and 9. However, a treatment of 70 or 80 °C for 10 min completely inactivated the phage. Moreover, morphologic analysis of vB_SauM_JS25 revealed that it was closely related to other Myoviridae phages infecting Staphylococcus species. The bacteriolytic activity of phage vB_SauM_JS25 at a multiplicity infection (MOI) 1 indicted its efficiency for reducing bacterial growth. These findings suggest that phage vB_SauM_JS25 could be considered a potential therapeutic or prophylactic candidate against S. aureus infection.
authors.affiliation: Key Open Laboratory of Edible Agricultural Products Safe Monitoring and Control, Ministry of Agriculture, Nanjing, 210014, People's Republic of China.
authors.forename: Lili
authors.initials: L
authors.lastname: Zhang
authors.forename: Hongduo
authors.initials: H
authors.lastname: Bao
authors.forename: Chengrui
authors.initials: C
authors.lastname: Wei
authors.forename: Hui
authors.initials: H
authors.lastname: Zhang
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhou
authors.forename: Ran
authors.initials: R
authors.lastname: Wang
mesh_terms: D000818:Animals; D001433:Bacteriolysis; D002417:Cattle; D002681:China; D015169:Colony Count, Microbial; D005260:Female; D058507:Host Specificity; D006863:Hydrogen-Ion Concentration; D008414:Mastitis, Bovine / Q000382:microbiology; D050296:Microbial Viability / Q000187:drug effects / Q000528:radiation effects; D046529:Microscopy, Electron, Transmission; D017900:Myoviridae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000648:ultrastructure; D017422:Sequence Analysis, DNA; D012722:Sewage / Q000821:virology; D013204:Staphylococcus Phages / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000648:ultrastructure; D013211:Staphylococcus aureus / Q000302:isolation & purification / Q000821:virology*; D013696:Temperature
chemical_list: D012722:Sewage