title: Ameliorative effect of myricetin on insulin resistance in mice fed a high-fat, high-sucrose diet.
abstract: OBJECTIVE
  Obesity-associated insulin resistance is a strong risk factor for type 2 diabetes mellitus. The aim of this study was to investigate the effect of myricetin on adiposity, insulin resistance, and inflammatory markers in mice with diet-induced insulin resistance.
  
  
  METHODS
  Five-week-old male C57BL/6J mice were fed a basal diet, a high-fat, high-sucrose (HFHS) diet, or the HFHS diet containing 0.06% myricetin or 0.12% myricetin for 12 weeks after a 1-week adaptation, and body weight and food intake were monitored. After sacrifice, serum lipid profiles, glucose, insulin, adipocyte-derived hormones, and proinflammatory cytokines were measured. The homeostasis model assessment for insulin resistance (HOMA-IR) was determined.
  
  
  RESULTS
  Myricetin given at 0.12% of the total diet significantly reduced body weight, weight gain, and epidydimal white adipose tissue weight, and improved hypertriglyceridemia and hypercholesterolemia without a significant influence on food intake in mice fed the HFHS diet. Serum glucose and insulin levels, as well as HOMA-IR values, decreased significantly by 0.12% myricetin supplementation in mice fed the HFHS diet. Myricetin given at 0.12% of the total diet significantly reduced serum levels of leptin, tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) in mice fed the HFHS diet.
  
  
  CONCLUSIONS
  These findings suggest that myricetin may have a protective effect against diet-induced obesity and insulin resistance in mice fed HFHS diet, and that alleviation of insulin resistance could partly occur by improving obesity and reducing serum proinflammatory cytokine levels.
authors.affiliation: Department of Smart Foods and Drugs, School of Food and Life Science, Inje University, 197 Inje-ro, Gimhae, Gyungnam 621-749, Korea.
authors.forename: Ha-Neul
authors.initials: HN
authors.lastname: Choi
authors.affiliation: Food & Nutrition Research Team, Hurom Co., Ltd., 79 Seobu-ro, Gimhae, Gyungnam 621-846, Korea.
authors.forename: Min-Jung
authors.initials: MJ
authors.lastname: Kang
authors.affiliation: Department of Smart Foods and Drugs, School of Food and Life Science, Inje University, 197 Inje-ro, Gimhae, Gyungnam 621-749, Korea.
authors.forename: Soo-Jin
authors.initials: SJ
authors.lastname: Lee
authors.affiliation: Department of Smart Foods and Drugs, School of Food and Life Science, Inje University, 197 Inje-ro, Gimhae, Gyungnam 621-749, Korea.
authors.forename: Jung-In
authors.initials: JI
authors.lastname: Kim
keywords: Myricetin; glucose; inflammation; insulin; obesity