title: Alterations in cardiac structure and function in a modified rat model of myocardial hypertrophy.
abstract: This study was aimed to establish a stable animal model of left ventricular hypertrophy (LVH) to provide theoretical and experimental basis for understanding the development of LVH. The abdominal aorta of male Wistar rats (80-100 g) was constricted to a diameter of 0.55 mm between the branches of the celiac and anterior mesenteric arteries. Echocardiography using a linear phased array probe was performed as well as pathological examination and plasma B-type natriuretic peptide (BNP) measurement at 3, 4 and 6 weeks after abdominal aortic constriction (AAC). The results showed that the acute mortality rate (within 24 h) of this modified rat model was 8%. Animals who underwent AAC demonstrated significantly increased interventricular septal (IVS), LV posterior wall (LVPWd), LV mass index (LVMI), cross-sectional area (CSA) of myocytes, and perivascular fibrosis; the ejection fraction (EF), fractional shortening (FS), and cardiac output (CO) were consistently lower at each time point after AAC. Notably, differences in these parameters between AAC group and sham group were significant by 3 weeks and reached peaks at 4th week. Following AAC, the plasma BNP was gradually elevated compared with the sham group at 3rd and 6th week. It was concluded that this modified AAC model can develop LVH, both stably and safely, by week four post-surgery; echocardiography is able to assess changes in chamber dimensions and systolic properties accurately in rats with LVH.
authors.affiliation: Department of Cardiology, The Second Affiliated Hospital of Guangzhou Medical University, Guangzhou, 510260, China.|Guangzhou Institute of Cardiovascular Disease, Guangzhou, 510260, China.
authors.forename: Wen-Jun
authors.initials: WJ
authors.lastname: Dai
authors.affiliation: Department of Physiology, Guangzhou Medical University, Guangzhou, 510182, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Dong
authors.affiliation: Guangzhou Institute of Cardiovascular Disease, Guangzhou, 510260, China. gzminsheng@vip.163.com.
authors.forename: Min-Sheng
authors.initials: MS
authors.lastname: Chen
authors.affiliation: Department of Medical Experimental Center, Guangzhou Medical University, Guangzhou, 510182, China.
authors.forename: Lu-Ning
authors.initials: LN
authors.lastname: Zhao
authors.affiliation: Department of Cardiology, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, 510120, China.
authors.forename: Ai-Lan
authors.initials: AL
authors.lastname: Chen
authors.affiliation: Department of Cardiology, The First Municipal People's Hospital of Guangzhou, Guangzhou Medical College, Guangzhou, 510180, China.
authors.forename: Zhen-Ci
authors.initials: ZC
authors.lastname: Li
authors.affiliation: Guangzhou Institute of Cardiovascular Disease, Guangzhou, 510260, China.
authors.forename: Shi-Ming
authors.initials: SM
authors.lastname: Liu
mesh_terms: D000818:Animals; D001012:Aorta, Abdominal / Q000473:pathology*; D003251:Constriction, Pathologic / Q000150:complications; D004195:Disease Models, Animal; D004452:Echocardiography / Q000379:methods; D004797:Enzyme-Linked Immunosorbent Assay; D006321:Heart / Q000503:physiopathology; D017379:Hypertrophy, Left Ventricular / Q000097:blood / Q000209:etiology / Q000473:pathology*; D008297:Male; D009206:Myocardium / Q000473:pathology; D020097:Natriuretic Peptide, Brain / Q000097:blood; D017208:Rats, Wistar; D013997:Time Factors
chemical_list: D020097:Natriuretic Peptide, Brain