title: 'Dilute-and-shoot' RSLC-MS-MS method for fast detection of nerve and vesicant chemical warfare agent metabolites in urine.
abstract: A sensitive screening method based on fast liquid chromatography tandem mass-spectrometry (RSLC-MS-MS) has shown the feasibility of separation and detection of low concentration β-lyase metabolites of sulfur mustard and of nerve agent phosphonic acids in urine. The analysis of these compounds is of interest because they are specific metabolites of the chemical warfare agents (CWAs), sulfur mustard (HD), sarin (GB), soman (GD), VX and Russian VX (RVX). The 'dilute-and-shoot' RSLC-MS-MS method provides a sensitive and direct approach for determining CWA exposure in non-extracted non-derivatized samples from urine. Chromatographic separation of the metabolites was achieved using a reverse phase column with gradient mobile phases consisting of 0.5% formic acid in water and acetonitrile. Identification and quantification of species were achieved using electrospray ionization-tandem mass-spectrometry monitoring two precursor-to-product ion transitions for each compound. The method demonstrates linearity over at least two orders of magnitude and had detection limits of 0.5 ng/mL in urine.
authors.affiliation: Department of Chemistry, Lomonosov Moscow State University, 119991 Moscow, Russia igorrodin@yandex.ru.
authors.forename: Igor
authors.initials: I
authors.lastname: Rodin
authors.affiliation: Department of Chemistry, Lomonosov Moscow State University, 119991 Moscow, Russia.
authors.forename: Arcady
authors.initials: A
authors.lastname: Braun
authors.affiliation: Department of Chemistry, Lomonosov Moscow State University, 119991 Moscow, Russia.
authors.forename: Andrey
authors.initials: A
authors.lastname: Stavrianidi
authors.affiliation: Department of Chemistry, Lomonosov Moscow State University, 119991 Moscow, Russia.
authors.forename: Timur
authors.initials: T
authors.lastname: Baygildiev
authors.affiliation: Department of Chemistry, Lomonosov Moscow State University, 119991 Moscow, Russia.
authors.forename: Oleg
authors.initials: O
authors.lastname: Shpigun
authors.affiliation: Laboratory for the Chemical and Analytical Control of Military Research Centre 105005, Brigadirsky Pereulok, 13, 105005 Moscow, Russia.
authors.forename: Dmitry
authors.initials: D
authors.lastname: Oreshkin
authors.affiliation: Laboratory for the Chemical and Analytical Control of Military Research Centre 105005, Brigadirsky Pereulok, 13, 105005 Moscow, Russia.
authors.forename: Igor
authors.initials: I
authors.lastname: Rybalchenko
mesh_terms: D002619:Chemical Warfare Agents / Q000032:analysis*; D002853:Chromatography, Liquid; D006801:Humans; D007509:Irritants / Q000652:urine*; D057230:Limit of Detection; D009151:Mustard Gas / Q000032:analysis; D009946:Organothiophosphorus Compounds / Q000652:urine; D011786:Quality Control; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D002619:Chemical Warfare Agents; D007509:Irritants; D009946:Organothiophosphorus Compounds; C451779:S-(N,N-diethylaminoethyl) isobutyl methylphosphothiolate; D009151:Mustard Gas