title: [Effect of NF-κB activation on the radiation response of esophageal cancer cells].
abstract: OBJECTIVE
  To investigate the effect of NF-κB activation on radiation response of esophageal carcinoma.
  
  
  METHODS
  The expression of NF-κB was detected in pretreatment and posttreatment specimens of patients with ESCC by immunohistochemistry. Electrophoretic mobility shift assay (EMSA) and Western blot were used to detect the activation of NF-κB in esophageal cancer cell line KYSE150 cells. SN50, a specific NF-κB inhibitor, was applied to inhibit the activation of NF-κB. Clone formation test was used to detect the radiosensitivity of esophageal cancer cells.
  
  
  RESULTS
  The median survival time of patients with activated and inactivated NF-κB in the pretreatment specimens were 16 and 19 months, respectively, with a non-significant difference between the two groups (P > 0.05). As to the patients with activated and inactivated NF-κB in posttreatment specimens, the median survival times were 13 and 35 months, respectively, with a significant difference (P < 0.01) between them. Western blot showed that the cytoplasmic expression of NF-κB was reduced with increasing radiation dose at 1.5 and 3 hours after radiation treatment. However, the expression of NF-κB in the cell nuclei was increased under the same condition, showing a trend of increased nucleus/cytoplasm ratio. The clone number in SN50 group was 96.66, 64.66, 76.66 and 10.00 under 0, 2, 4 and 12 Gy irradiation, which demonstrated a significant difference compared with the control groups (P < 0.001).
  
  
  CONCLUSIONS
  Our results show that activation of NF-κB is induced by radiotherapy. Activation of NF-κB reduces the outcome of radiation treatment of esophageal cancer patients.
authors.affiliation: Department of Thoracic Surgery, Cancer Hospital/Institute, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100021, China.
authors.forename: Baozhong
authors.initials: B
authors.lastname: Li
authors.affiliation: Department of Thoracic Surgery, Cancer Hospital/Institute, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100021, China.
authors.forename: Zhaoli
authors.initials: Z
authors.lastname: Chen
authors.affiliation: Department of Thoracic Surgery, Cancer Hospital/Institute, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100021, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Zhou
authors.affiliation: Department of Thoracic Surgery, Cancer Hospital/Institute, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100021, China. Email: prof.hejie@263.net.
authors.forename: Jie
authors.initials: J
authors.lastname: He
mesh_terms: D015153:Blotting, Western; D045744:Cell Line, Tumor; D002467:Cell Nucleus; D004938:Esophageal Neoplasms / Q000378:metabolism / Q000532:radiotherapy*; D006801:Humans; D007150:Immunohistochemistry; D016328:NF-kappa B / Q000378:metabolism*; D011836:Radiation Tolerance; D015398:Signal Transduction
chemical_list: D016328:NF-kappa B