title: [The construction and application of a novel apparatus for detecting oxygen consumption of mice under normobaric hypoxia].
abstract: OBJECTIVE
  To establish a method for real-time recording the oxygen consumption of mice under normobaric hypoxia.
  
  
  METHODS
  The experimental apparatus was made up of animal container, filling water control system, electronic balance, hose, a computer with weight recording software, etc. The working principle was that the oxygen consumed by animal was replaced by water filling which was controlled by the pneumatic and hydraulic actuator. The water was weighted by an electronic balance and the weight signal was recorded into excel file at the same time. The accuracy and precision of the apparatus were detected by a 10 ml syringe. The oxygen consumption characteristics of 6 acute repetitive hypoxia mice and 6 normal mice were observed.
  
  
  RESULTS
  The P value for the paired t test was 1 and the CV value was 4%. The survival time and total oxygen consumption of acute repetitive hypoxia mice were both significantly increased compared to normal mice (P < 0.05), which were (58.8 +/- 6.8) min and (46.0 +/- 8.7) min respectively for the survival time and (85.1 +/- 8.5) ml and (73.6 +/- 5.4) ml respectively for total oxygen consumption.
  
  
  CONCLUSIONS
  The hypoxia tolerance of the acute repetitive hypoxia mice can significantly improved by taking more oxygen in the animal cabin. The accuracy and precision of the apparatus are high and it can be used for the determination of oxygen consumption in hypoxia research.
authors.forename: Rui-Feng
authors.initials: RF
authors.lastname: Duan
authors.forename: Xiang-Zhi
authors.initials: XZ
authors.lastname: Zeng
authors.forename: Jia-Li
authors.initials: JL
authors.lastname: Ji
authors.forename: Zhi-Qing
authors.initials: ZQ
authors.lastname: Zhang
authors.forename: Yan-Fang
authors.initials: YF
authors.lastname: Zhang
authors.forename: Chao-Liang
authors.initials: CL
authors.lastname: Long
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.forename: Wen-Yu
authors.initials: WY
authors.lastname: Cui
authors.forename: Hai
authors.initials: H
authors.lastname: Wang
mesh_terms: D000818:Animals; D000860:Hypoxia / Q000503:physiopathology*; D051379:Mice; D008991:Monitoring, Physiologic / Q000295:instrumentation*; D010101:Oxygen Consumption / Q000502:physiology*