title: Cloning and characterization of two subunits of calcineurin cDNA in naked carp (Gymnocypris przewalskii) from Lake Qinghai, China.
abstract: The naked carp (Gymnocypris przewalskii), a native teleost, plays an important role in maintenance of the ecological balance in the system of Lake Qinghai (altitude, 3.2 km) on the Qinghai-Tibet Plateau in China. Calcineurin (CN) is the only member of the serine/threonine phosphatase family that can be activated by both Ca2+ and calmodulin (CaM) and involved in many important physiological processes such as salt tolerance/adaption. In this report, cDNAs of CN catalytic subunit paralogue isoforms: GpCAα (GenBank accession no.JQ407043), GpCAγ (GenBank accession no. JQ407043), and CN regulatory subunit (GpCB) (GenBank accession no. JQ410473), were isolated from Gymnocypris przewalskii and their expression patterns in embryos developmentwere characterized. Gene expression profile demonstrated that GpCA and GpCB mRNA was distributed ubiquitously in all embryonic stages and showed decline until final stage of development. Immunohistologicalanalysis revealed CN localization in different tissues including kidney, heart, brain, spermary, and gill. Collectively, these results provide molecular basis and clues to further understand the role of CN during embryos development and its function in tissues for the adaptation mechanism of naked carp.
authors.forename: Fulei
authors.initials: F
authors.lastname: Wei
authors.forename: Chenlong
authors.initials: C
authors.lastname: Wang
authors.forename: Zhaoxi
authors.initials: Z
authors.lastname: Wang
authors.forename: Yawei
authors.initials: Y
authors.lastname: Kong
authors.forename: Yuan
authors.initials: Y
authors.lastname: Shi
authors.forename: Jianquan
authors.initials: J
authors.lastname: Shi
authors.forename: Delin
authors.initials: D
authors.lastname: Qi
authors.forename: Hongfang
authors.initials: H
authors.lastname: Qi
authors.forename: Jian
authors.initials: J
authors.lastname: Liang
authors.forename: Rongqing
authors.initials: R
authors.lastname: Zhang
authors.affiliation: College of Eco-Environmental Engineering, Qinghai University, Xining, China. lichangzhong@qhu.edu.cn.
authors.forename: Changzhong
authors.initials: C
authors.lastname: Li
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D019703:Calcineurin / Q000737:chemistry / Q000235:genetics*; D002347:Carps / Q000196:embryology / Q000235:genetics*; D002681:China; D003001:Cloning, Molecular; D018076:DNA, Complementary; D018507:Gene Expression Regulation, Developmental; D007150:Immunohistochemistry; D008969:Molecular Sequence Data; D021122:Protein Subunits / Q000235:genetics / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D014018:Tissue Distribution; D059467:Transcriptome
chemical_list: D018076:DNA, Complementary; D021122:Protein Subunits; D019703:Calcineurin