title: Biohybrid networks of selectively desulfated glycosaminoglycans for tunable growth factor delivery.
abstract: Sulfation patterns of glycosaminoglycans (GAG) govern the electrostatic complexation of biomolecules and thus allow for modulating the release profiles of growth factors from GAG-based hydrogels. To explore options related to this, selectively desulfated heparin derivatives were prepared, thoroughly characterized, and covalently converted with star-shaped poly(ethylene glycol) into binary polymer networks. The impact of the GAG sulfation pattern on the network characteristics of the obtained hydrogels was theoretically evaluated by mean field methods and experimentally analyzed by rheometry and swelling measurements. Sulfation-dependent differences of reactivity and miscibility of the heparin derivatives were shown to determine network formation. A theory-based design concept for customizing growth factor affinity and physical characteristics was introduced and validated by quantifying the release of fibroblast growth factor 2 from a set of biohybrid gels. The resulting new class of cell-instructive polymer matrices with tunable GAG sulfation will be instrumental for multiple applications in biotechnology and medicine.
authors.affiliation: Leibniz Institute of Polymer Research Dresden , Hohe Strasse 6, 01069 Dresden, Germany.
authors.forename: Andrea
authors.initials: A
authors.lastname: Zieris
authors.forename: Ron
authors.initials: R
authors.lastname: Dockhorn
authors.forename: Anika
authors.initials: A
authors.lastname: Röhrich
authors.forename: Ralf
authors.initials: R
authors.lastname: Zimmermann
authors.forename: Martin
authors.initials: M
authors.lastname: Müller
authors.forename: Petra B
authors.initials: PB
authors.lastname: Welzel
authors.forename: Mikhail V
authors.initials: MV
authors.lastname: Tsurkan
authors.forename: Jens-Uwe
authors.initials: JU
authors.lastname: Sommer
authors.forename: Uwe
authors.initials: U
authors.lastname: Freudenberg
authors.forename: Carsten
authors.initials: C
authors.lastname: Werner
mesh_terms: D055598:Chemical Phenomena; D004337:Drug Carriers / Q000737:chemistry*; D016222:Fibroblast Growth Factor 2 / Q000737:chemistry*; D006025:Glycosaminoglycans / Q000737:chemistry*; D006493:Heparin / Q000737:chemistry; D020100:Hydrogels / Q000737:chemistry; D011092:Polyethylene Glycols / Q000737:chemistry; D013002:Somatomedins / Q000737:chemistry*; D013055:Spectrophotometry, Infrared; D017550:Spectroscopy, Fourier Transform Infrared; D055672:Static Electricity
chemical_list: D004337:Drug Carriers; D006025:Glycosaminoglycans; D020100:Hydrogels; D013002:Somatomedins; D016222:Fibroblast Growth Factor 2; D011092:Polyethylene Glycols; D006493:Heparin