title: Evaluation of chromosomal damage, cytostasis, cytotoxicity, oxidative DNA damage and their association with body-mass index in obese subjects.
abstract: Over-weight and obesity are serious problems that increase the risk not only for chronic diseases like diabetes and heart disease but also of various types of cancer. This study was conducted to evaluate cytokinesis-block micronucleus cytome (CBMN-cyt) assay parameters and plasma concentrations of 8-hydroxy-2'-deoxyguanosine (8-OHdG), and their relationship with age, body-mass index (BMI) and waist-to-hip ratio (WHR) in 83 obese, 21 over-weight and 21 normal-weight subjects. Frequencies of micronuclei (MN), nucleoplasmic bridges (NPB), nuclear buds (NBUD), and apoptotic and necrotic cells in lymphocytes of obese subjects were found to be significantly higher than those found in normal-weight and over-weight subjects (p<0.01 and p<0.05), whereas plasma concentrations of 8-OHdG in obese subjects were lower than those observed in normal-weight and over-weight subjects (p<0.05 and p<0.01, respectively). There was a negative correlation between age and frequency of necrotic cells and NDI (p<0.05), whereas there was no correlation between BMI, WHR, CBMN cyt assay parameters and plasma 8-OHdG in normal-weight subjects. In over-weight subjects, a negative correlation was observed between age and NDI (p<0.01) and a positive correlation between age and frequency of NPB (p<0.01) and between BMI and frequency of NBUD (p<0.05). In obese subjects, a negative correlation was observed between age and NDI (p<0.01) and between BMI and NDI (p<0.05), whereas no correlation was observed between WHR and CBMN-cyt assay parameters and plasma 8-OHdG. However, frequencies of MN, NPB, NBUD, apoptotic and necrotic cells in total over-weight/obese (p<0.01/p<0.05) and all subjects (p<0.01) increased with increasing BMI. The increase in genomic damage (MN, NPB and NBUD) in obese subjects and the positive correlation between genomic damage and BMI in total over-weight/obese subjects indicate that obesity increases genomic damage and may be associated with an increased risk of cancer, because an increase in MN frequency is a predictor of cancer risk.
authors.affiliation: Department of Medical Biology, Faculty of Medicine, Erciyes University, Kayseri, Turkey. Electronic address: donmezh@erciyes.edu.tr.
authors.forename: Hamiyet
authors.initials: H
authors.lastname: Donmez-Altuntas
authors.affiliation: Department of Medical Biology, Faculty of Medicine, Erciyes University, Kayseri, Turkey.
authors.forename: Fatma
authors.initials: F
authors.lastname: Sahin
authors.affiliation: Department of Endocrinology and Metabolism, Faculty of Medicine, Erciyes University, Kayseri, Turkey.
authors.forename: Fahri
authors.initials: F
authors.lastname: Bayram
authors.affiliation: Department of Medical Biology, Faculty of Medicine, Erciyes University, Kayseri, Turkey.
authors.forename: Nazmiye
authors.initials: N
authors.lastname: Bitgen
authors.affiliation: Department of Endocrinology and Metabolism, Kayseri Education and Research Hospital, Kayseri, Turkey.
authors.forename: Meral
authors.initials: M
authors.lastname: Mert
authors.affiliation: Department of Biochemistry, Faculty of Medicine, Erciyes University, Kayseri, Turkey.
authors.forename: Kenan
authors.initials: K
authors.lastname: Guclu
authors.affiliation: Department of Medical Biology, Faculty of Medicine, Erciyes University, Kayseri, Turkey.
authors.forename: Zuhal
authors.initials: Z
authors.lastname: Hamurcu
authors.affiliation: Department of Endocrinology and Metabolism, Faculty of Medicine, Erciyes University, Kayseri, Turkey.
authors.forename: Sülbiye
authors.initials: S
authors.lastname: Arıbas
authors.affiliation: Intensive Care Unit, Department of Internal Medicine, Faculty of Medicine, Erciyes University, Kayseri, Turkey.
authors.forename: Kursat
authors.initials: K
authors.lastname: Gundogan
authors.affiliation: Department of Endocrinology and Metabolism, Faculty of Medicine, Erciyes University, Kayseri, Turkey.
authors.forename: Halit
authors.initials: H
authors.lastname: Diri
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D000328:Adult; D015992:Body Mass Index; D016022:Case-Control Studies; D049109:Cell Proliferation; D002478:Cells, Cultured; D002869:Chromosome Aberrations / Q000706:statistics & numerical data; D004249:DNA Damage; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000097:blood; D005260:Female; D006801:Humans; D008297:Male; D015162:Micronucleus Tests; D008875:Middle Aged; D009765:Obesity / Q000453:epidemiology / Q000235:genetics / Q000378:metabolism / Q000503:physiopathology; D018384:Oxidative Stress; D055815:Young Adult
chemical_list: D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine
keywords: 8-OHdG concentration; BMI; CBMN cyt assay; MN frequency; Obesity; WHR