title: [Study on extracting and separating curcuminoids from Curcuma longa rhizome using ultrasound strengthen by microemulsion].
abstract: OBJECTIVE
  To establish a new method for the extraction and separation of curcuminoids from Curcuma longa rhizome by cloud-point preconcentration using microemulsions as solvent.
  
  
  METHODS
  The spectrophotometry was used to detect the solubility of curcumin in different oil phase, emulsifier and auxiliary emulsifier, and the microemulsion prescription was used for false three-phase figure optimization. The extraction process was optimized by uniform experiment design. The curcuminoids were separated from microemulsion extract by cloud-point preconcentration.
  
  
  RESULTS
  Oil phase was oleic acid ethyl ester; Emulsifier was OP emulsifier; Auxiliary emulsifier was polyethylene glycol(peg) 400; The quantity of emulsifier to auxiliary emulsifier was the ratio of 5: 1; Microemulsion prescription was water-oleic acid ethyl ester-mixed emulsifier (0.45:0.1:0.45). The optimum extraction process was: time for 12.5 min, temperature of 52 degrees C, power of 360 W, frequency of 400 kHz, and the liquid-solid ratio of 40:1. The extraction rate of curcuminoids was 92.17% and 86.85% in microemulsion and oil phase, respectively.
  
  
  CONCLUSIONS
  Curcuminoids is soluble in this microemulsion prescription with good extraction rate. This method is simple and suitable for curcuminoids extraction from Curcuma longa rhizome.
authors.forename: Chun-Hua
authors.initials: CH
authors.lastname: Yue
authors.forename: Li-Tao
authors.initials: LT
authors.lastname: Zheng
authors.forename: Qi-Ming
authors.initials: QM
authors.lastname: Guo
authors.forename: Kun-Ping
authors.initials: KP
authors.lastname: Li
mesh_terms: D030024:Curcuma / Q000737:chemistry*; D003474:Curcumin / Q000031:analogs & derivatives* / Q000737:chemistry / Q000302:isolation & purification*; D004365:Drugs, Chinese Herbal / Q000737:chemistry; D045703:Emulsifying Agents / Q000737:chemistry; D004655:Emulsions / Q000737:chemistry; D010938:Plant Oils / Q000737:chemistry; D027343:Rhizome / Q000737:chemistry; D013501:Surface-Active Agents / Q000737:chemistry; D013678:Technology, Pharmaceutical / Q000379:methods; D014465:Ultrasonics
chemical_list: D004365:Drugs, Chinese Herbal; D045703:Emulsifying Agents; D004655:Emulsions; D010938:Plant Oils; D013501:Surface-Active Agents; D003474:Curcumin