title: Essential oil from the leaves of Xylopia langsdorfiana (Annonaceae) as a possible spasmolytic agent.
abstract: Xylopia langsdorfiana A. St.-Hil. &Tul. (Annonaceae) is popularly known in the northeast of Brazil as 'pimenteira da terra', and an essential oil (XL-OE) was extracted from its leaves. Since Xylopia species are cited in folk medicine and diterpenes from X. langsdorfiana have spasmolytic activity, this study aimed to investigate a possible spasmolytic action of XL-OE on smooth muscle models. XL-OE (243 and 729 μg/mL) showed low pharmacologic efficacy on guinea pig trachea and rat aorta and uterus. However, in guinea pig ileum, XL-OE (27-729 μg/mL) inhibited carbachol or histamine-induced phasic contractions (1 μM) in a significant and concentration-dependent manner. In addition, XL-OE (81 μg/mL) reduced fluorescence intensity in ileal myocytes stimulated by histamine, indicating a decrease in cytosolic calcium concentration, which could explain the spasmolytic activity. Thus, XL-OE proved to be a promising natural product to be used in gastrointestinal diseases acting by modulating the cytosolic calcium concentration.
authors.affiliation: a Center for Health Sciences (CCS), Federal University of Paraíba (UFPB) , Campus I, 58.051-970, PO Box 5009, João Pessoa , Paraíba , Brazil.
authors.forename: Ana Carolina de C
authors.initials: AC
authors.lastname: Correia
authors.forename: Tamyris F
authors.initials: TF
authors.lastname: Ferreira
authors.forename: Italo Rossi R
authors.initials: IR
authors.lastname: Martins
authors.forename: Cibério L
authors.initials: CL
authors.lastname: Macêdo
authors.forename: Fabio de S
authors.initials: Fde S
authors.lastname: Monteiro
authors.forename: Vicente Carlos O
authors.initials: VC
authors.lastname: Costa
authors.forename: Josean F
authors.initials: JF
authors.lastname: Tavares
authors.forename: Marcelo S
authors.initials: MS
authors.lastname: Silva
authors.forename: Edgar J
authors.initials: EJ
authors.lastname: Paredes-Gamero
authors.forename: Marcus V
authors.initials: MV
authors.lastname: Buri
authors.forename: Vera L S
authors.initials: VL
authors.lastname: Rigoni
authors.forename: Viviane L A
authors.initials: VL
authors.lastname: Nouailhetas
authors.forename: Bagnólia A
authors.initials: BA
authors.lastname: Da Silva
mesh_terms: D000818:Animals; D001011:Aorta / Q000187:drug effects; D002118:Calcium / Q000737:chemistry; D003600:Cytosol / Q000737:chemistry; D004224:Diterpenes / Q000737:chemistry; D005260:Female; D006168:Guinea Pigs; D007082:Ileum / Q000187:drug effects; D032342:Muscle Cells / Q000187:drug effects; D009119:Muscle Contraction / Q000187:drug effects; D009130:Muscle, Smooth / Q000187:drug effects; D009822:Oils, Volatile / Q000737:chemistry*; D010276:Parasympatholytics / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry; D010938:Plant Oils / Q000737:chemistry*; D051381:Rats; D014132:Trachea / Q000187:drug effects; D014599:Uterus / Q000187:drug effects; D054336:Xylopia / Q000737:chemistry*
chemical_list: D004224:Diterpenes; D009822:Oils, Volatile; D010276:Parasympatholytics; D010938:Plant Oils; D002118:Calcium
keywords: Annonaceae; antispasmodic; calcium; essential oil; smooth muscle