title: "Multiplexed viability, cytotoxicity, and caspase activity assays".
abstract: Multiplexed assay chemistries provide for multiple measurements of cellular parameters within a single assay well. This experimental practice is not only more cost efficient, but also provides more information about a compound or treatment. The ability to combine the activity profiles within the same sample provides a level of normalization not possible with parallel assays. Furthermore, multiplexing caspase activity assays with viability and/or cytotoxicity assays can support conclusions regarding cytotoxic mechanism and provide normalization, which may help correct for differences in cell number.
authors.affiliation: Promega Corporation, 2800 Woods Hollow Road, Madison, WI, 53711, USA, andrew.niles@promega.com.
authors.forename: Andrew L
authors.initials: AL
authors.lastname: Niles
authors.forename: Terry L
authors.initials: TL
authors.lastname: Riss
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D001897:Boronic Acids / Q000494:pharmacology; D000069286:Bortezomib; D020169:Caspases / Q000032:analysis* / Q000378:metabolism*; D018929:Cell Culture Techniques; D004789:Enzyme Activation; D006801:Humans; D020014:K562 Cells / Q000187:drug effects; D008967:Molecular Biology / Q000295:instrumentation / Q000379:methods*; D011719:Pyrazines / Q000494:pharmacology; D018675:Toxicity Tests
chemical_list: D001897:Boronic Acids; D011719:Pyrazines; D000069286:Bortezomib; D020169:Caspases