title: [Effect of interleukin-18 gene modified human umbilical cord mesenchymal stem cells on proliferation of breast cancer cell].
abstract: OBJECTIVE
  To establish human umbilical cord mesenchymal stem cells (HUMSCs) strain transfected with interleukin-18 (IL-18) gene and examine its effects on the proliferation of breast cancer cell (MCF-7).
  
  
  METHODS
  HUMSCs were isolated and cultured. And the lentivirus-IL-18 vector containing human IL-18 gene was constructed and transfected into HUMSCs. The expressions of IL-18 gene mRNA and protein were detected by semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) and Western blot. After co-culturing for 1, 3, 5 days, Transwell and cell counting kit-8 (CCK-8) assays were performed on MCF-7 to plot the cell growth curve.
  
  
  RESULTS
  IL-18-HUMSCs could stably express of IL-18 gene and inhibit the proliferation of breast cancer cells. The IL-18 mRNA relative expression amounts were 1.40 ± 0.21 for experimental group (IL-18-HUMSCs), 0.59 ± 0.09 for negative control group (NV-HUMSCs) and 0.71 ± 0.05 for blank control group (HUMSCs). As compared with control group, the difference was statistically significant (F = 31.81, P = 0.001). The relative expressions of IL-18 protein were 1.54 ± 0.27 for experimental group (IL-18-HUMSCs), 0.57 ± 0.04 for negative control group (NV-HUMSCs) and 0.59 ± 0.23 for blank control group (HUMSCs). As compared with control group, the difference was statistically significant (F = 22.32, P = 0.002). After co-culturing for 5 days, the cellular proliferation was significantly inhibited.
  
  
  CONCLUSIONS
  IL-18 gene has been successfully transfected into HUMSCs and has a stable expression. And IL-18-HUMSCs can effectively inhibit the proliferation of breast cancer cells in vitro.
authors.affiliation: Diagnosis and Treatment Centre for Breast Disease, Affiliated Hospital of Medical College, Qingdao University, Qingdao 266071, China.
authors.forename: Suyuan
authors.initials: S
authors.lastname: Sun
authors.affiliation: Diagnosis and Treatment Centre for Breast Disease, Affiliated Hospital of Medical College, Qingdao University, Qingdao 266071, China.
authors.forename: Xiaoyi
authors.initials: X
authors.lastname: Liu
authors.affiliation: Diagnosis and Treatment Centre for Breast Disease, Affiliated Hospital of Medical College, Qingdao University, Qingdao 266071, China.
authors.forename: Dandan
authors.initials: D
authors.lastname: Jiang
authors.affiliation: Diagnosis and Treatment Centre for Breast Disease, Affiliated Hospital of Medical College, Qingdao University, Qingdao 266071, China.
authors.forename: Zhidong
authors.initials: Z
authors.lastname: Lü
authors.affiliation: Diagnosis and Treatment Centre for Breast Disease, Affiliated Hospital of Medical College, Qingdao University, Qingdao 266071, China. Email: drlifunian@aliyun.com.
authors.forename: Funian
authors.initials: F
authors.lastname: Li
mesh_terms: D001943:Breast Neoplasms / Q000473:pathology*; D002453:Cell Cycle; D049109:Cell Proliferation; D018920:Coculture Techniques; D005822:Genetic Vectors; D006801:Humans; D020382:Interleukin-18 / Q000235:genetics*; D059630:Mesenchymal Stem Cells / Q000378:metabolism*; D012333:RNA, Messenger; D014162:Transfection; D014470:Umbilical Cord
chemical_list: C000589760:IL18 protein, human; D020382:Interleukin-18; D012333:RNA, Messenger