title: Rapid determination of major bioactive isoflavonoid compounds during the extraction process of kudzu (Pueraria lobata) by near-infrared transmission spectroscopy.
abstract: Near-infrared (NIR) spectroscopy has been developed into an indispensable tool for both academic research and industrial quality control in a wide field of applications. The feasibility of NIR spectroscopy to monitor the concentration of puerarin, daidzin, daidzein and total isoflavonoid (TIF) during the extraction process of kudzu (Pueraria lobata) was verified in this work. NIR spectra were collected in transmission mode and pretreated with smoothing and derivative. Partial least square regression (PLSR) was used to establish calibration models. Three different variable selection methods, including correlation coefficient method, interval partial least squares (iPLS), and successive projections algorithm (SPA) were performed and compared with models based on all of the variables. The results showed that the approach was very efficient and environmentally friendly for rapid determination of the four quality indices (QIs) in the kudzu extraction process. This method established may have the potential to be used as a process analytical technological (PAT) tool in the future.
authors.affiliation: National Glycoengineering Research Center and School of Pharmaceutical Science, Shandong University, N0. 44 West Wenhua Road, Jinan 250012, China; School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China.
authors.forename: Pei
authors.initials: P
authors.lastname: Wang
authors.affiliation: Beijing Kaiyuan Shengshi Science and Technology Development Co., LTD., Jinan 250012, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Zhang
authors.affiliation: National Glycoengineering Research Center and School of Pharmaceutical Science, Shandong University, N0. 44 West Wenhua Road, Jinan 250012, China.
authors.forename: Hailong
authors.initials: H
authors.lastname: Yang
authors.affiliation: National Glycoengineering Research Center and School of Pharmaceutical Science, Shandong University, N0. 44 West Wenhua Road, Jinan 250012, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Nie
authors.affiliation: National Glycoengineering Research Center and School of Pharmaceutical Science, Shandong University, N0. 44 West Wenhua Road, Jinan 250012, China. Electronic address: zanghcw@126.com.
authors.forename: Hengchang
authors.initials: H
authors.lastname: Zang
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000191:economics / Q000379:methods; D007529:Isoflavones / Q000032:analysis* / Q000302:isolation & purification; D016018:Least-Squares Analysis; D018517:Plant Roots / Q000737:chemistry*; D029908:Pueraria / Q000737:chemistry*; D019265:Spectroscopy, Near-Infrared / Q000191:economics / Q000379:methods*
chemical_list: D007529:Isoflavones; C013908:daidzin; C004742:daidzein; C033607:puerarin
keywords: High performance liquid chromatography; Kudzu; Near-infrared spectroscopy; Partial least squares regression