title: A mini-electrochemical system integrated micropipet tip and pencil graphite electrode for detection of anticancer drug sensitivity in vitro.
abstract: Developing a reliable and cost-effective miniaturized electroanalysis tool is of vital importance for cell electrochemical analysis. In this work, a novel mini-electrochemical system has been constructed for trace detection of cell samples. The mini-electrochemical system was constructed by integrating a pencil graphite modified by threonine (PT/PGE) as working electrode, an Ag/AgCl (Sat'd) as reference electrode, platinum wire as counter electrode and a micropipet tip as electrochemical cell. The mini-electrochemical system not only saved dramatically usage of samples from 500 μL in traditional electrochemical system to 10 μL, but also possessed an adjustable active surface area by changing the length of PT/PGE immersed into the cell suspension from 3mm to 15 mm, and the linear equation was ipa = 2.25 l-2.64 (R(2) = 0.990). The system was successfully used in detection of MCF-7 cells, and a nonlinear exponent relationship between peak current and the cell number range from 3.0 × l0(3) to 7.0 × l0(6) cells mL(-1) was established firstly with the index equation ipa = 59.557 e (-C/1.709)-71.486 (R(2) = 0.954). Finally, the system was used for evaluating the sensitivity of cyclophosphamide on MCF-7 cell, and the result was corresponded well with that of MTT assay. The proposed system is sufficiently simple, cheap and easy operated, and could be applied in electrochemical detection of other biological samples.
authors.affiliation: Department of Chemistry, Harbin Institute of Technology, Harbin, 150001, China; College of Pharmacy, Jiamusi University, Jiamusi, 154000, China.
authors.forename: Xiaoling
authors.initials: X
authors.lastname: Guo
authors.affiliation: College of Pharmacy, Jiamusi University, Jiamusi, 154000, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Wang
authors.affiliation: College of Pharmacy, Jiamusi University, Jiamusi, 154000, China.
authors.forename: Jinlian
authors.initials: J
authors.lastname: Li
authors.affiliation: College of Pharmacy, Jiamusi University, Jiamusi, 154000, China.
authors.forename: Jiwen
authors.initials: J
authors.lastname: Cui
authors.affiliation: College of Pharmacy, Jiamusi University, Jiamusi, 154000, China.
authors.forename: Shi
authors.initials: S
authors.lastname: Zhou
authors.affiliation: Department of Chemistry, Harbin Institute of Technology, Harbin, 150001, China. Electronic address: haosue@hit.edu.cn.
authors.forename: Sue
authors.initials: S
authors.lastname: Hao
authors.affiliation: College of Pharmacy, Jiamusi University, Jiamusi, 154000, China. Electronic address: dmwu@jmsu.edu.cn.
authors.forename: Dongmei
authors.initials: D
authors.lastname: Wu
mesh_terms: D018906:Antineoplastic Agents, Alkylating / Q000008:administration & dosage; D017209:Apoptosis / Q000187:drug effects*; D003217:Conductometry / Q000295:instrumentation*; D003520:Cyclophosphamide / Q000008:administration & dosage*; D004305:Dose-Response Relationship, Drug; D004354:Drug Screening Assays, Antitumor / Q000295:instrumentation*; D004566:Electrodes; D004867:Equipment Design; D019544:Equipment Failure Analysis; D017022:Flow Injection Analysis / Q000295:instrumentation; D006108:Graphite / Q000737:chemistry*; D006801:Humans; D061986:MCF-7 Cells; D055617:Micro-Electrical-Mechanical Systems / Q000295:instrumentation*; D008845:Microinjections / Q000295:instrumentation*; D008904:Miniaturization; D018511:Systems Integration
chemical_list: D018906:Antineoplastic Agents, Alkylating; D006108:Graphite; D003520:Cyclophosphamide
keywords: Anticancer drug sensitivity; MCF-7 cell; Micropipet tip; Mini-electrochemical system; Pencil graphite electrode