title: A sensitive and quantitative fluorescent multi-component immuno-chromatographic sensor for β-agonist residues.
abstract: A sensitive and quantitative fluorescent multi-component immuno-chromatographic sensor was developed for detection of three β-agonizts: clenbuterol, ractopamine and salbuterol. A competitive immune strategy between antibody conjugated fluorescent beads and β-agonist or their antigens was employed. Each monoclonal antibody specifically recognizes it is corresponding β-agonist in the conjugating zone. The unreacted antibodies were captured by β-agonist antigens immobilized at three test lines in nitrocellulose membrane reaction zone. This enables simultaneous detection of 3 β-agonizts in one single test without any further sample preparation. The test results can be obtained within 10 min. Limit of detections for clenbuterol, ractopamine and salbuterol were 0.10 ng/mL, 0.10 ng/mL and 0.09 ng/mL, respectively. Recoveries ranged from 70.0% to 100.5% and relative standard deviations were below 15%. The assay was evaluated using spiked and real samples and the results were compared with LC-MS/MS. The developed novel assay method provides a low cost, sensitive and rapid approach for on site detection of β-agonizts.
authors.affiliation: Key Laboratory of Agro-product Safety and Quality, Ministry of Agriculture, Beijing 100081, PR China; Institute of Quality Standards & Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Beijing 100081, PR China. Electronic address: wplcon99@163.com.
authors.forename: Peilong
authors.initials: P
authors.lastname: Wang
authors.affiliation: Beijing Zhifeng Botai Bio-technology Co. LTD., Beijing 102200, PR China.
authors.forename: Zhi
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Key Laboratory of Agro-product Safety and Quality, Ministry of Agriculture, Beijing 100081, PR China; Institute of Quality Standards & Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Beijing 100081, PR China. Electronic address: suxiaoou@caas.cn.
authors.forename: Xiaoou
authors.initials: X
authors.lastname: Su
mesh_terms: D000318:Adrenergic beta-Agonists / Q000032:analysis* / Q000276:immunology; D000818:Animals; D015374:Biosensing Techniques / Q000295:instrumentation*; D002846:Chromatography, Affinity / Q000295:instrumentation*; D045424:Complex Mixtures / Q000032:analysis / Q000276:immunology; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005504:Food Analysis / Q000295:instrumentation*; D005506:Food Contamination / Q000032:analysis*; D013050:Spectrometry, Fluorescence / Q000295:instrumentation*; D013552:Swine
chemical_list: D000318:Adrenergic beta-Agonists; D045424:Complex Mixtures
keywords: Fluorescent bead; Immuno-chromatographic sensor; Multi-analyte detection; β-Agonist