title: Multifunctional carbon nanohorn complexes for cancer treatment.
abstract: Multifunctional carbon nanohorn (CNH) complexes were synthesized using oxidized CNH, magnetite (MAG) nanoparticles, and polyethyleneimine (PEI). The ferromagnetic MAG nanoparticles were loaded onto CNH (MAG-CNH) using iron(II) acetate and subsequent heat treatment. Chemical functionalization of the MAG-CNH complexes with PEI improved their water-dispersibility and allowed further conjugation with a fluorophore. The application of an external magnetic field significantly intensified the targeted vectorization of CNH complexes into human cervical cancer (HeLa) cells. Following cell uptake, laser irradiation of the cells showed a significant enhancement in the photothermal effects of CNHs leading to cell death. We have confirmed that the photothermal properties and ferromagnetic characteristics of CNH complexes show efficient cell elimination. The present study is an essential step toward the development of an innovative cancer therapy and a highly sensitive detection of cancer cells at the single-cell level.
authors.affiliation: Nanotube Research Center (NTRC), National Institute of Advanced Industrial Science and Technology (AIST), Central 5, 1-1-1 Higashi, Tsukuba 305-8565 (Japan).
authors.forename: Svetlana A
authors.initials: SA
authors.lastname: Chechetka
authors.forename: Benoit
authors.initials: B
authors.lastname: Pichon
authors.forename: Minfang
authors.initials: M
authors.lastname: Zhang
authors.forename: Masako
authors.initials: M
authors.lastname: Yudasaka
authors.forename: Sylvie
authors.initials: S
authors.lastname: Bégin-Colin
authors.forename: Alberto
authors.initials: A
authors.lastname: Bianco
authors.forename: Eijiro
authors.initials: E
authors.lastname: Miyako
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use; D002244:Carbon / Q000737:chemistry*; D002470:Cell Survival / Q000187:drug effects; D005456:Fluorescent Dyes / Q000737:chemistry; D006367:HeLa Cells; D006801:Humans; D007834:Lasers; D058185:Magnetite Nanoparticles / Q000737:chemistry; D049329:Nanostructures / Q000737:chemistry* / Q000627:therapeutic use; D011094:Polyethyleneimine / Q000737:chemistry
chemical_list: D000970:Antineoplastic Agents; D005456:Fluorescent Dyes; D058185:Magnetite Nanoparticles; D002244:Carbon; D011094:Polyethyleneimine
keywords: cancer; nanomaterials; nanoparticles; nanotubes; near infrared