title: Analysis of hyaluronan synthase activity.
abstract: Hyaluronan (HA) is a component of the extracellular matrix that is involved in many physiological and pathological processes. As HA modulates several functions (i.e., cell proliferation and migration, inflammation), its presence in the tissues can have positive or negative effects. HA synthases (HAS) are a family of three isoenzymes located on the plasma membrane that are responsible for the production of such polysaccharide and, therefore, their activity is critical to determine the accumulation of HA in tissues. Here, we describe a nonradioactive method to quantify the HAS enzymatic activity in crude cellular membrane preparation.
authors.affiliation: Dipartimento di Scienze Chirurgiche e Morfologiche, Università degli Studi dell'Insubria, via J.H. Dunant 5, 21100, Varese, Italy.
authors.forename: Davide
authors.initials: D
authors.lastname: Vigetti
authors.forename: Evgenia
authors.initials: E
authors.lastname: Karousou
authors.forename: Manuela
authors.initials: M
authors.lastname: Viola
authors.forename: Alberto
authors.initials: A
authors.lastname: Passi
mesh_terms: D002462:Cell Membrane / Q000201:enzymology; D002851:Chromatography, High Pressure Liquid; D004187:Disaccharides / Q000378:metabolism; D057075:Enzyme Assays / Q000379:methods*; D014453:Glucuronosyltransferase / Q000378:metabolism*; D000076002:Hyaluronan Synthases
chemical_list: D004187:Disaccharides; D014453:Glucuronosyltransferase; D000076002:Hyaluronan Synthases