title: Quantum dot-based multiphoton fluorescent pipettes for targeted neuronal electrophysiology.
abstract: Targeting visually identified neurons for electrophysiological recording is a fundamental neuroscience technique; however, its potential is hampered by poor visualization of pipette tips in deep brain tissue. We describe quantum dot-coated glass pipettes that provide strong two-photon contrast at deeper penetration depths than those achievable with current methods. We demonstrated the pipettes' utility in targeted patch-clamp recording experiments and single-cell electroporation of identified rat and mouse neurons in vitro and in vivo.
authors.affiliation: MTA KOKI Lendület Laboratory of Neuronal Signaling, Institute of Experimental Medicine, Hungarian Academy of Sciences, Budapest, Hungary.
authors.forename: Bertalan K
authors.initials: BK
authors.lastname: Andrásfalvy
authors.affiliation: Department of Basic Neurosciences, University of Geneva, Geneva, Switzerland.
authors.forename: Gregorio L
authors.initials: GL
authors.lastname: Galiñanes
authors.affiliation: Department of Basic Neurosciences, University of Geneva, Geneva, Switzerland.
authors.forename: Daniel
authors.initials: D
authors.lastname: Huber
authors.affiliation: Applied Physics and Instrumentation Group, Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia, USA.
authors.forename: Mladen
authors.initials: M
authors.lastname: Barbic
authors.affiliation: Applied Physics and Instrumentation Group, Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia, USA.
authors.forename: John J
authors.initials: JJ
authors.lastname: Macklin
authors.affiliation: Optical Sciences Division, Code 5611, U.S. Naval Research Laboratory, Washington, District of Columbia, USA.|Sotera Defense Solutions, Annapolis Junction, Maryland, USA.
authors.forename: Kimihiro
authors.initials: K
authors.lastname: Susumu
authors.affiliation: Center for Bio/Molecular Science and Engineering, Code 6900, U.S. Naval Research Laboratory, Washington, District of Columbia, USA.
authors.forename: James B
authors.initials: JB
authors.lastname: Delehanty
authors.affiliation: Optical Sciences Division, Code 5611, U.S. Naval Research Laboratory, Washington, District of Columbia, USA.
authors.forename: Alan L
authors.initials: AL
authors.lastname: Huston
authors.affiliation: MTA KOKI Lendület Laboratory of Neuronal Signaling, Institute of Experimental Medicine, Hungarian Academy of Sciences, Budapest, Hungary.
authors.forename: Judit K
authors.initials: JK
authors.lastname: Makara
authors.affiliation: Center for Bio/Molecular Science and Engineering, Code 6900, U.S. Naval Research Laboratory, Washington, District of Columbia, USA.
authors.forename: Igor L
authors.initials: IL
authors.lastname: Medintz
mesh_terms: D000818:Animals; D001921:Brain / Q000166:cytology / Q000502:physiology*; D004594:Electrophysiology / Q000295:instrumentation / Q000379:methods*; D005456:Fluorescent Dyes; D008164:Luminescent Proteins / Q000378:metabolism*; D051379:Mice; D008856:Microscopy, Fluorescence / Q000295:instrumentation / Q000379:methods*; D009474:Neurons / Q000166:cytology / Q000502:physiology*; D055095:Optics and Photonics / Q000295:instrumentation* / Q000379:methods; D018408:Patch-Clamp Techniques / Q000295:instrumentation / Q000379:methods*; D045663:Quantum Dots; D051381:Rats
chemical_list: D005456:Fluorescent Dyes; D008164:Luminescent Proteins