title: Surface-independent antibacterial coating using silver nanoparticle-generating engineered mussel glue.
abstract: During implant surgeries, antibacterial agents are needed to prevent bacterial infections, which can cause the formation of biofilms between implanted materials and tissue. Mussel adhesive proteins (MAPs) derived from marine mussels are bioadhesives that show strong adhesion and coating ability on various surfaces even in wet environment. Here, we proposed a novel surface-independent antibacterial coating strategy based on the fusion of MAP to a silver-binding peptide, which can synthesize silver nanoparticles having broad antibacterial activity. This sticky recombinant fusion protein enabled the efficient coating on target surface and the easy generation of silver nanoparticles on the coated-surface under mild condition. The biosynthesized silver nanoparticles showed excellent antibacterial efficacy against both Gram-positive and Gram-negative bacteria and also revealed good cytocompatibility with mammalian cells. In this coating strategy, MAP-silver binding peptide fusion proteins provide hybrid environment incorporating inorganic silver nanoparticle and simultaneously mediate the interaction of silver nanoparticle with surroundings. Moreover, the silver nanoparticles were fully synthesized on various surfaces including metal, plastic, and glass by a simple, surface-independent coating manner, and they were also successfully synthesized on a nanofiber surface fabricated by electrospinning of the fusion protein. Thus, this facile surface-independent silver nanoparticle-generating antibacterial coating has great potential to be used for the prevention of bacterial infection in diverse biomedical fields.
authors.affiliation: Department of Chemical Engineering, Pohang University of Science and Technology , Pohang 790-784, Korea.
authors.forename: Yun Kee
authors.initials: YK
authors.lastname: Jo
authors.forename: Jeong Hyun
authors.initials: JH
authors.lastname: Seo
authors.forename: Bong-Hyuk
authors.initials: BH
authors.lastname: Choi
authors.forename: Bum Jin
authors.initials: BJ
authors.lastname: Kim
authors.forename: Hwa Hui
authors.initials: HH
authors.lastname: Shin
authors.forename: Byeong Hee
authors.initials: BH
authors.lastname: Hwang
authors.forename: Hyung Joon
authors.initials: HJ
authors.lastname: Cha
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000494:pharmacology; D049872:Bivalvia / Q000378:metabolism; D002460:Cell Line; D020099:Coated Materials, Biocompatible / Q000737:chemistry* / Q000378:metabolism; D006090:Gram-Negative Bacteria / Q000187:drug effects; D006094:Gram-Positive Bacteria / Q000187:drug effects; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000633:toxicity; D051379:Mice; D057139:Nanofibers / Q000737:chemistry / Q000648:ultrastructure; D010455:Peptides / Q000235:genetics / Q000378:metabolism; D011137:Polystyrenes / Q000737:chemistry; D011506:Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics; D012835:Silver Nitrate / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D000900:Anti-Bacterial Agents; D020099:Coated Materials, Biocompatible; D010455:Peptides; D011137:Polystyrenes; D011506:Proteins; D011993:Recombinant Fusion Proteins; C037403:adhesive protein, mussel; D012835:Silver Nitrate
keywords: antibacterial coating; mussel adhesive protein; silver binding peptide; silver nanoparticle; surface-independent coating