title: Suppression of bacterial wilt of tomato by bioorganic fertilizer made from the antibacterial compound producing strain Bacillus amyloliquefaciens HR62.
abstract: Ralstonia solanacearum (Smith) is an important soil-borne pathogen worldwide. We investigated the effects of a new bioorganic fertilizer, BIO62, which was made from organic fertilizer and antagonist Bacillus amyloliquefaciens HR62, on the control of bacterial wilt of tomato in greenhouse condition. The results showed that the application of BIO62 significantly decreased disease incidence by 65% and strongly reduced R. solanacearum populations both in the rhizosphere soil (8.04 log cfu g(-1) dry soil) and crown sections (5.63 log cfu g(-1) fresh plant section) at 28 days after pathogen challenge. Antibacterial compounds produced by HR62 were purified by silica gel, Sephadex LH-20, and HPLC and then identified using HPLC/electrospray ionization mass spectrometry analysis. Macrolactin A and 7-O-malonyl macrolactin A (molecular weights of 402 and 488 Da, respectively), along with surfactin B (molecular weights of 994, 1008, 1022, and 1036 Da), were observed to inhibit the growth of R. solanacearum.
authors.affiliation: National Engineering Research Center for Organic-Based Fertilizers and Jiangsu Collaborative Innovation Center for Solid Organic Waste Utilization, Nanjing Agricultural University , Nanjing, 210095, China.
authors.forename: Jianfeng
authors.initials: J
authors.lastname: Huang
authors.forename: Zhong
authors.initials: Z
authors.lastname: Wei
authors.forename: Shiyong
authors.initials: S
authors.lastname: Tan
authors.forename: Xinlan
authors.initials: X
authors.lastname: Mei
authors.forename: Qirong
authors.initials: Q
authors.lastname: Shen
authors.forename: Yangchun
authors.initials: Y
authors.lastname: Xu
mesh_terms: D000900:Anti-Bacterial Agents / Q000032:analysis / Q000378:metabolism / Q000494:pharmacology; D000898:Antibiosis; D001407:Bacillus / Q000737:chemistry / Q000378:metabolism / Q000502:physiology*; D005308:Fertilizers / Q000032:analysis*; D018551:Solanum lycopersicum / Q000382:microbiology*; D010935:Plant Diseases / Q000382:microbiology* / Q000517:prevention & control; D043368:Ralstonia solanacearum / Q000502:physiology*
chemical_list: D000900:Anti-Bacterial Agents; D005308:Fertilizers
keywords: Ralstonia solanacearum; antibacterial compounds; bacterial wilt of tomato; biological control; bioorganic fertilizer