title: Remodeling of the thoracic aorta after bone marrow cell transplantation.
abstract: Stem cells are characterized by their ability to differentiate into multiple cell lineages and display the paracrine effect. The aim of this work was to evaluate the effect of therapy with bone marrow cells (BMCs) on blood glucose, lipid metabolism and aortic wall remodeling in mice through the administration of a high fat diet and subsequent BMCs transplantation. C57BL/6 mice were fed a control diet (CO group) or an atherogenic diet (AT group). After 16 weeks, the AT group was divided into four groups: an AT 14 days group and AT 21 days group, that were given an injection of vehicle and sacrificed at 14 and 21 days after, respectively; AT-BMC 14 days group and AT-BMC 21 days group that was given an injection of BMCs and sacrificed at 14 and 21 days after. The CO group was sacrificed along with other groups. The BMCs transplant had reduced blood glucose, triglycerides and total cholesterol. The Qa (1/mm(2)) was quantitatively reduced in AT 14 days group, AT 21 days group and was high in AT-BMC 21 days group. The AT 21 days group exhibited increased tunica media and elastic system fibers. The immunolabeling for α-SMA and VEGF showed less immunolabeling in transplanted groups with BMCs. The immunostaining for PCNA seems to be more expressive in the group AT-BMC 21 days group. To conclude, our results support the concept that in mice, the injection of BMCs improve glucose levels, lipid metabolism and remodeling of the aortic wall in animals using atherogenic diet.
authors.affiliation: Laboratory of Ultrastructure and Tissue Biology, Institute of Biology Roberto Alcantara Gomes, State University of Rio de Janeiro Rio de Janeiro, Brazil.
authors.forename: Alyne
authors.initials: A
authors.lastname: Felix
authors.affiliation: Laboratory of Ultrastructure and Tissue Biology, Institute of Biology Roberto Alcantara Gomes, State University of Rio de Janeiro Rio de Janeiro, Brazil.
authors.forename: Nemesis
authors.initials: N
authors.lastname: Monteiro
authors.affiliation: Laboratory of Ultrastructure and Tissue Biology, Institute of Biology Roberto Alcantara Gomes, State University of Rio de Janeiro Rio de Janeiro, Brazil.
authors.forename: Vinícius Novaes
authors.initials: VN
authors.lastname: Rocha
authors.affiliation: Laboratory of Cell Culture, Institute of Biology Roberto Alcantara Gomes, State University of Rio de Janeiro Rio de Janeiro, Brazil.
authors.forename: Genilza
authors.initials: G
authors.lastname: Oliveira
authors.affiliation: Laboratory of Ultrastructure and Tissue Biology, Institute of Biology Roberto Alcantara Gomes, State University of Rio de Janeiro Rio de Janeiro, Brazil.
authors.forename: Alan Cesar
authors.initials: AC
authors.lastname: Moraes
authors.affiliation: Laboratory of Ultrastructure and Tissue Biology, Institute of Biology Roberto Alcantara Gomes, State University of Rio de Janeiro Rio de Janeiro, Brazil.
authors.forename: Cherley
authors.initials: C
authors.lastname: Andrade
authors.affiliation: Laboratory of Ultrastructure and Tissue Biology, Institute of Biology Roberto Alcantara Gomes, State University of Rio de Janeiro Rio de Janeiro, Brazil.
authors.forename: Ana Lucia
authors.initials: AL
authors.lastname: Nascimento
authors.affiliation: Laboratory of Cell Culture, Institute of Biology Roberto Alcantara Gomes, State University of Rio de Janeiro Rio de Janeiro, Brazil.
authors.forename: Laís
authors.initials: L
authors.lastname: de Carvalho
authors.affiliation: Laboratory of Cell Culture, Institute of Biology Roberto Alcantara Gomes, State University of Rio de Janeiro Rio de Janeiro, Brazil.
authors.forename: Alessandra
authors.initials: A
authors.lastname: Thole
authors.affiliation: Laboratory of Ultrastructure and Tissue Biology, Institute of Biology Roberto Alcantara Gomes, State University of Rio de Janeiro Rio de Janeiro, Brazil.
authors.forename: Jorge
authors.initials: J
authors.lastname: Carvalho
mesh_terms: D000199:Actins / Q000378:metabolism; D000818:Animals; D001013:Aorta, Thoracic / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology*; D001018:Aortic Diseases / Q000097:blood / Q000209:etiology / Q000473:pathology / Q000503:physiopathology / Q000601:surgery*; D050197:Atherosclerosis / Q000097:blood / Q000209:etiology / Q000473:pathology / Q000503:physiopathology / Q000601:surgery*; D015415:Biomarkers / Q000097:blood; D001786:Blood Glucose / Q000378:metabolism; D016026:Bone Marrow Transplantation; D059305:Diet, High-Fat; D004195:Disease Models, Animal; D008055:Lipids / Q000097:blood; D008297:Male; D008810:Mice, Inbred C57BL; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D013997:Time Factors; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism; D066253:Vascular Remodeling
chemical_list: C558396:Acta2 protein, mouse; D000199:Actins; D015415:Biomarkers; D001786:Blood Glucose; D008055:Lipids; D018809:Proliferating Cell Nuclear Antigen; D042461:Vascular Endothelial Growth Factor A; C467480:vascular endothelial growth factor A, mouse
keywords: Bone marrow cells; aorta remodeling; atherogenic diet