title: Development of a biocompatible nanodelivery system for tuberculosis drugs based on isoniazid-Mg/Al layered double hydroxide.
abstract: The primary challenge in finding a treatment for tuberculosis (TB) is patient non-compliance to treatment due to long treatment duration, high dosing frequency, and adverse effects of anti-TB drugs. This study reports on the development of a nanodelivery system that intercalates the anti-TB drug isoniazid into Mg/Al layered double hydroxides (LDHs). Isoniazid was found to be released in a sustained manner from the novel nanodelivery system in humans in simulated phosphate buffer solutions at pH 4.8 and pH 7.4. The nanodelivery formulation was highly biocompatible compared to free isoniazid against human normal lung and 3T3 mouse fibroblast cells. The formulation was active against Mycobacterium tuberculosis and gram-positive bacteria and gram-negative bacteria. Thus results show significant promise for the further study of these nanocomposites for the treatment of TB.
authors.affiliation: Materials Synthesis and Characterization Laboratory, Institute of Advanced Technology, Universiti Putra Malaysia, Serdang, Selangor, Malaysia.
authors.forename: Bullo
authors.initials: B
authors.lastname: Saifullah
authors.affiliation: Laboratory of Vaccines and Immunotherapeutics, Institute of Bioscience, Universiti Putra Malaysia, Serdang, Selangor, Malaysia.
authors.forename: Palanisamy
authors.initials: P
authors.lastname: Arulselvan
authors.affiliation: Laboratory of Vaccines and Immunotherapeutics, Institute of Bioscience, Universiti Putra Malaysia, Serdang, Selangor, Malaysia ; Department of Environmental Health, Faculty of Public Health and Tropical Medicine, Jazan University, Jazan, Saudi Arabia.
authors.forename: Mohamed Ezzat
authors.initials: ME
authors.lastname: El Zowalaty
authors.affiliation: Laboratory of Vaccines and Immunotherapeutics, Institute of Bioscience, Universiti Putra Malaysia, Serdang, Selangor, Malaysia ; Department of Human Anatomy, Faculty of Medicine and Health Science, Universiti Putra Malaysia, Serdang, Selangor, Malaysia.
authors.forename: Sharida
authors.initials: S
authors.lastname: Fakurazi
authors.affiliation: Department of Chemical Engineering and Program in Bioengineering, Northeastern University, Boston, MA, USA ; Center of Excellence for Advanced Materials Research, King Abdulaziz University, Jeddah, Saudi Arabia.
authors.forename: Thomas J
authors.initials: TJ
authors.lastname: Webster
authors.affiliation: Department of Chemical Engineering and Program in Bioengineering, Northeastern University, Boston, MA, USA.
authors.forename: Benjamin M
authors.initials: BM
authors.lastname: Geilich
authors.affiliation: Materials Synthesis and Characterization Laboratory, Institute of Advanced Technology, Universiti Putra Malaysia, Serdang, Selangor, Malaysia.
authors.forename: Mohd Zobir
authors.initials: MZ
authors.lastname: Hussein
mesh_terms: D000536:Aluminum Hydroxide / Q000737:chemistry* / Q000633:toxicity; D000818:Animals; D000995:Antitubercular Agents / Q000737:chemistry* / Q000493:pharmacokinetics* / Q000494:pharmacology / Q000633:toxicity; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D004338:Drug Combinations; D016503:Drug Delivery Systems / Q000379:methods*; D006801:Humans; D007538:Isoniazid / Q000737:chemistry* / Q000493:pharmacokinetics* / Q000494:pharmacology / Q000633:toxicity; D008276:Magnesium Hydroxide / Q000737:chemistry* / Q000633:toxicity; D051379:Mice; D009169:Mycobacterium tuberculosis / Q000187:drug effects; D041681:NIH 3T3 Cells; D053761:Nanocomposites / Q000737:chemistry*
chemical_list: D000995:Antitubercular Agents; D004338:Drug Combinations; C013591:aluminum hydroxide, magnesium hydroxide, drug combination; D000536:Aluminum Hydroxide; D008276:Magnesium Hydroxide; D007538:Isoniazid
keywords: Mg/Al LDH; isoniazid; nanodelivery system; tuberculosis