title: Differential protein expression in the susceptible and resistant Myzus persicae (Sulzer) to imidacloprid.
abstract: Myzus persicae, a serious economic agricultural pest, has developed resistance to imidacloprid (IMI), which was widely used to control this aphid worldwide. To gain a better understanding of the mechanisms of IMI resistance in M. persicae, we carried out a comparative proteomic analysis. Total proteins of the IMI-susceptible and resistant strains were extracted and separated by two-dimensional gel electrophoresis. More than 1300 protein spots were reproducibly detected, including 14 that were more abundant and 14 less abundant. Mass spectrometry analysis and database searching helped us to identify 25 differentially abundant proteins. The identified proteins were categorized into several functional groups including signal transduction, RNA processing, protein processing, transport processing, stress response, metabolisms, and cytoskeleton structure, etc. This study is the first analysis of differentially expressed proteins in IMI-susceptible and resistant M. Persicae, and gives new insights into the mechanisms of IMI resistance in M. persicae.
authors.affiliation: Guizhou Tobacco Science Research Institute, Guiyang 550081, China.
authors.forename: JianYu
authors.initials: J
authors.lastname: Meng
authors.affiliation: College of Agriculture, Guizhou University, Guiyang 550025, China.
authors.forename: ChangYu
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Guizhou Tobacco Science Research Institute, Guiyang 550081, China.
authors.forename: XingJiang
authors.initials: X
authors.lastname: Chen
authors.affiliation: Guizhou Tobacco Science Research Institute, Guiyang 550081, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Cao
authors.affiliation: Guizhou Tobacco Science Research Institute, Guiyang 550081, China. Electronic address: insect0417@163.com.
authors.forename: ShengHua
authors.initials: S
authors.lastname: Shang
mesh_terms: D000818:Animals; D001042:Aphids / Q000187:drug effects* / Q000235:genetics* / Q000378:metabolism; D007093:Imidazoles / Q000494:pharmacology*; D019476:Insect Proteins / Q000235:genetics* / Q000378:metabolism; D007305:Insecticide Resistance; D007306:Insecticides / Q000494:pharmacology*; D000073943:Neonicotinoids; D009574:Nitro Compounds / Q000494:pharmacology*
chemical_list: D007093:Imidazoles; D019476:Insect Proteins; D007306:Insecticides; D000073943:Neonicotinoids; D009574:Nitro Compounds; C082359:imidacloprid
keywords: Imidacloprid; Myzus persicae; Proteomics; Two-dimensional gel electrophoresis