title: Cytochrome P450s from the fall armyworm (Spodoptera frugiperda): responses to plant allelochemicals and pesticides.
abstract: Spodoptera frugiperda is a polyphagous lepidopteran pest that encounters a wide range of toxic plant metabolites in its diet. The ability of this insect to adapt to its chemical environment might be explained by the action of major detoxification enzymes such as cytochrome P450s (or CYP). Forty-two sequences coding for P450s were identified and most of the transcripts were found to be expressed in the midgut, Malpighian tubules and fat body of S. frugiperda larvae. Relatively few P450s were expressed in the established cell line Sf9. In order to gain information on how these genes respond to different chemical compounds, larvae and Sf9 cells were exposed to plant secondary metabolites (indole, indole-3-carbinol, quercetin, 2-tridecanone and xanthotoxin), insecticides (deltamethrin, fipronil, methoprene, methoxyfenozide) or model inducers (clofibrate and phenobarbital). Several genes were induced by plant chemicals such as P450s from the 6B, 321A and 9A subfamilies. Only a few genes responded to insecticides, belonging principally to the CYP9A family. There was little overlap between the response in vivo measured in the midgut and the response in vitro in Sf9 cells. In addition, regulatory elements were detected in the promoter region of these genes. In conclusion, several P450s were identified that could potentially be involved in the adaptation of S. frugiperda to its chemical environment.
authors.affiliation: INRA, UMR 1355, Institut Sophia Agrobiotech, Sophia-Antipolis, France; CNRS, UMR 7254, Sophia-Antipolis, France; Université de Nice Sophia Antipolis, Sophia-Antipolis, France; Environment Canada, Centre Saint-Laurent, Montreal, QC, Canada.
authors.forename: M
authors.initials: M
authors.lastname: Giraudo
authors.forename: F
authors.initials: F
authors.lastname: Hilliou
authors.forename: T
authors.initials: T
authors.lastname: Fricaux
authors.forename: P
authors.initials: P
authors.lastname: Audant
authors.forename: R
authors.initials: R
authors.lastname: Feyereisen
authors.forename: G
authors.initials: G
authors.lastname: Le Goff
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D065693:Cytochrome P-450 Enzyme Inducers / Q000494:pharmacology*; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D004064:Digestive System / Q000201:enzymology; D005216:Fat Body / Q000201:enzymology; D020869:Gene Expression Profiling; D007306:Insecticides / Q000494:pharmacology*; D007814:Larva / Q000201:enzymology; D008317:Malpighian Tubules / Q000201:enzymology; D008969:Molecular Sequence Data; D010675:Pheromones / Q000494:pharmacology*; D010944:Plants / Q000737:chemistry; D060888:Real-Time Polymerase Chain Reaction; D018411:Spodoptera / Q000201:enzymology* / Q000235:genetics*
chemical_list: D065693:Cytochrome P-450 Enzyme Inducers; D007306:Insecticides; D010675:Pheromones; D003577:Cytochrome P-450 Enzyme System
keywords: Spodoptera frugiperda; cytochrome P450s; gene expression induction; xenobiotics