title: Functional improvements in β-lactoglobulin by conjugating with soybean soluble polysaccharide.
abstract: Soybean soluble polysaccharide (SSPS) was hydrolyzed by autoclaving for 6 and 12 h to obtain SSPS (6 h) and SSPS (12 h). Bovine β-lactoglobulin (BLG) was conjugated with each SSPS by the Maillard reaction to improve its function. Conjugation between BLG and each SSPS was confirmed by Sodium dodecyl sulfate polyacrylamide gel electrophoresis and isoelectric focusing. BLG-SSPS (6 h) and BLG-SSPS (12 h), respectively, retained approximately 56 and 43% of the retinol-binding activity of BLG. Structural analyses by intrinsic fluorescence and enzyme-linked immunosorbent assay with monoclonal antibodies indicated that the native structure of BLG had almost been maintained in each conjugate and that the surface structure was covered by conjugated SSPS. The emulsifying properties of BLG were improved in each conjugate at pH 3, 5, and 7 in the presence of 0.2 M NaCl. The antibody responses to BLG-SSPS (6 h) and BLG-SSPS (12 h) were considerably reduced in BALB/c mice. We conclude that conjugation with SSPS was very effective for improving the function of BLG and this study would contribute to greater utilization of SSPS.
authors.affiliation: a Department of Applied Biological Science , Tokyo University of Agriculture and Technology , Tokyo , Japan.
authors.forename: Naoki
authors.initials: N
authors.lastname: Inada
authors.forename: Mai
authors.initials: M
authors.lastname: Hayashi
authors.forename: Tadashi
authors.initials: T
authors.lastname: Yoshida
authors.forename: Makoto
authors.initials: M
authors.lastname: Hattori
mesh_terms: D000818:Animals; D000906:Antibodies / Q000097:blood*; D002417:Cattle; D004591:Electrophoresis, Polyacrylamide Gel; D004655:Emulsions; D005260:Female; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D007114:Immunization; D007525:Isoelectric Focusing; D007782:Lactoglobulins / Q000008:administration & dosage / Q000737:chemistry* / Q000276:immunology; D015416:Maillard Reaction; D051379:Mice; D008807:Mice, Inbred BALB C; D016269:Milk Hypersensitivity / Q000097:blood / Q000276:immunology / Q000473:pathology / Q000517:prevention & control*; D011134:Polysaccharides / Q000737:chemistry* / Q000276:immunology; D011485:Protein Binding; D012965:Sodium Chloride; D013025:Glycine max / Q000737:chemistry*; D014801:Vitamin A / Q000737:chemistry
chemical_list: D000906:Antibodies; D004655:Emulsions; D007782:Lactoglobulins; D011134:Polysaccharides; D014801:Vitamin A; D012965:Sodium Chloride
keywords: functional improvements; neoglycoconjugate; protein conjugation; soybean soluble polysaccharide; β-lactoglobulin