title: The interfering effects of superovulation and vitrification upon some important epigenetic biomarkers in mouse blastocyst.
abstract: Appropriate epigenetic changes in preimplantation embryos are critical for embryonic development and successful pregnancy. The aim of this study was to evaluate the effects of some assisted reproductive techniques (ARTs) on a panel of epigenetic biomarkers by immunofluorescence staining at blastocyst stage. For this purpose, four treatment groups were designed: control (C), superovulation (S), superovulation+in vitro culture (SI), and superovulation+vitrification+in vitro culture (SVI). Results showed that vitrification decreased the developmental competence of embryos cultured in vitro (P<0.05). Semi-quantitative analysis revealed that vitrification decreased the fluorescence intensity of global DNA methylation in the inner cell mass (ICM), in SVI Group in comparison to C group (P<0.05). Superovulation, elevated the level of H3K9acetylation of trophectoderm (TE) in comparison to C and SI groups (P<0.05). Furthermore, ARTs manipulations influenced H3K9acetylation in the ICM (P<0.05). The fluorescence intensity of H4K12acetylation in TE for SVI group was higher than C and S (P<0.05). For H3K4tri-methylation, S group had higher fluorescence intensity in the ICM in comparison to SI and SVI (P<0.05). Finally, in vitro culture decreased Pou5f1 protein signal in comparison to in vivo-derived embryos at blastocyst stage (P<0.05). In conclusion, ART manipulations may have important influences on multiple epigenetic biomarkers.
authors.affiliation: Department of Animal Sciences, College of Agriculture, Isfahan University of Technology, Isfahan 8415683111, Iran.
authors.forename: Azizollah
authors.initials: A
authors.lastname: Bakhtari
authors.affiliation: Department of Animal Sciences, College of Agriculture, Isfahan University of Technology, Isfahan 8415683111, Iran.
authors.forename: Hamid-Reza
authors.initials: HR
authors.lastname: Rahmani
authors.affiliation: Department of Animal Sciences, College of Agriculture, Isfahan University of Technology, Isfahan 8415683111, Iran.
authors.forename: Elham
authors.initials: E
authors.lastname: Bonakdar
authors.affiliation: Department of Reproductive Biotechnology at Reproductive Biomedicine Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran.
authors.forename: Farnoosh
authors.initials: F
authors.lastname: Jafarpour
authors.affiliation: Department of Reproductive Biotechnology at Reproductive Biomedicine Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran.
authors.forename: Vajiheh
authors.initials: V
authors.lastname: Asgari
authors.affiliation: Department of Reproductive Biotechnology at Reproductive Biomedicine Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran.
authors.forename: Sayyed-Mortaza
authors.initials: SM
authors.lastname: Hosseini
authors.affiliation: Department of Reproductive Biotechnology at Reproductive Biomedicine Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran.
authors.forename: Mehdi
authors.initials: M
authors.lastname: Hajian
authors.affiliation: Department of Animal Sciences, College of Agriculture, Isfahan University of Technology, Isfahan 8415683111, Iran.
authors.forename: Mohammad-Ali
authors.initials: MA
authors.lastname: Edriss
authors.affiliation: Department of Reproductive Biotechnology at Reproductive Biomedicine Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran. Electronic address: mh.nasr-esfahani@royaninstitute.org.
authors.forename: Mohammad-Hossein
authors.initials: MH
authors.lastname: Nasr-Esfahani
mesh_terms: D000107:Acetylation; D000818:Animals; D001755:Blastocyst / Q000166:cytology* / Q000378:metabolism; D015925:Cryopreservation; D019175:DNA Methylation; D046149:Embryo Culture Techniques; D047108:Embryonic Development; D044127:Epigenesis, Genetic; D005260:Female; D006657:Histones / Q000032:analysis / Q000378:metabolism; D008297:Male; D008745:Methylation; D051379:Mice; D050814:Octamer Transcription Factor-3 / Q000032:analysis / Q000378:metabolism; D011247:Pregnancy; D013480:Superovulation; D058989:Vitrification
chemical_list: D006657:Histones; D050814:Octamer Transcription Factor-3; C494174:Pou5f1 protein, mouse
keywords: ART manipulations; DNA methylation; H3K4 tri-methylation; H3K9 acetylation; H4K12 acetylation; Pou5f1