title: Convenient detection of metal-DNA, metal-RNA, and metal-protein adducts with a click-modified Pt(II) complex.
abstract: cis-[Pt(2-azido-1,3-propanediamine)Cl2] is a reagent for high-yield post-treatment fluorescent labelling of Pt(II) biomolecular targets using click chemistry and exhibits a bias in conformational isomers in the context of duplex DNA. Pt-protein adducts are detected using BSA as a model. Following in vivo treatment, long-lived Pt-RNA adducts are detected on ribosomal RNA.
authors.affiliation: Department of Chemistry & Biochemistry, University of Oregon, Eugene, OR 97403-1253, USA.
authors.forename: Alan D
authors.initials: AD
authors.lastname: Moghaddam
authors.forename: Jonathan D
authors.initials: JD
authors.lastname: White
authors.forename: Rachael M
authors.initials: RM
authors.lastname: Cunningham
authors.forename: Andrea N
authors.initials: AN
authors.lastname: Loes
authors.forename: Michael M
authors.initials: MM
authors.lastname: Haley
authors.forename: Victoria J
authors.initials: VJ
authors.lastname: DeRose
mesh_terms: D000818:Animals; D002417:Cattle; D057930:Click Chemistry; D056831:Coordination Complexes / Q000138:chemical synthesis / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D018736:DNA Adducts / Q000737:chemistry; D006834:Hydrazines / Q000737:chemistry; D010984:Platinum / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry* / Q000378:metabolism; D012313:RNA / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry / Q000378:metabolism
chemical_list: C417561:Alexa 488 hydrazide; D056831:Coordination Complexes; D018736:DNA Adducts; D006834:Hydrazines; D011506:Proteins; D012710:Serum Albumin, Bovine; D010984:Platinum; D012313:RNA; D004247:DNA