title: Susceptibility and progression of end stage renal disease are not associated with angiotensin II type 1 receptor gene polymorphism.
abstract: BACKGROUND
  The role of the angiotensin II type 1 receptor (AT1R) gene polymorphism, A1166C, has been shown to be associated with end stage renal disease (ESRD) and its progression. There is also some evidence that HLA class II alleles are associated with ESRD independent of other factors.
  
  
  OBJECTIVE
  To examine the association between AT1R gene polymorphism in the susceptibility and progression to ESRD in patients with chronic renal failure and to investigate if the AT1R genotypes and HLA-DR alleles predict the time to ESRD.
  
  
  METHODS
  Genotyping was performed in 50 ESRD patients and 44 control subjects for the AT1R A1166C gene polymorphism using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). ESRD patients were examined for HLA-DRB1 alleles according to a reverse hybridization line probe assay.
  
  
  RESULTS
  Allele and genotype frequencies of the AT1R polymorphism did not differ significantly between ESRD patients and controls. Furthermore, there was no association between the AT1R gene polymorphism or HLA-DRB1 alleles with the time to the occurrence of end stage failure.
  
  
  CONCLUSIONS
  We concluded that the AT1R genotype does not contribute to the genetic susceptibility of ESRD and is not associated with progression of chronic kidney failure to ESRD.
authors.affiliation: a Department of Clinical Pathology and.
authors.forename: Mariam Onsy F
authors.initials: MO
authors.lastname: Hanna
authors.affiliation: a Department of Clinical Pathology and.
authors.forename: Rasha Mohamad Hosny
authors.initials: RM
authors.lastname: Shahin
authors.affiliation: a Department of Clinical Pathology and.
authors.forename: Safa S
authors.initials: SS
authors.lastname: Meshaal
authors.affiliation: b Department of Internal Medicine , Faculty of Medicine, Cairo University , Kasr Al Ainy , Cairo , Egypt.
authors.forename: Inas F
authors.initials: IF
authors.lastname: Kostandi
mesh_terms: D000328:Adult; D001483:Base Sequence; D004534:Egypt / Q000453:epidemiology; D005260:Female; D056726:Genetic Association Studies; D005819:Genetic Markers / Q000235:genetics; D020022:Genetic Predisposition to Disease / Q000453:epidemiology* / Q000235:genetics*; D006801:Humans; D007676:Kidney Failure, Chronic / Q000453:epidemiology* / Q000235:genetics*; D008297:Male; D008969:Molecular Sequence Data; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D015995:Prevalence; D044140:Receptor, Angiotensin, Type 1 / Q000235:genetics*; D015203:Reproducibility of Results; D012307:Risk Factors; D012680:Sensitivity and Specificity
chemical_list: C544065:AGTR1 protein, human; D005819:Genetic Markers; D044140:Receptor, Angiotensin, Type 1
keywords: Angiotensin II type 1 receptor; DNA polymorphism; end stage renal disease