title: In vitro antioxidant activity and total phenolic content of endophytic fungi isolated from Eugenia jambolana Lam.
abstract: OBJECTIVE
  To elucidate the antioxidant activity and total phenolic content (TPC) of ethyl acetate extracts of endophytic fungi isolated from Eugenia jambolana by three different antioxidant assays.
  
  
  METHODS
  Twenty one different endophytic fungal extracts were screened for presence of various phytochemicals, TPC and in vitro antioxidant activity. TPC was tested by Folin-Ciocalteau reagent based assay. DPPH free radical scavenging, hydrogen peroxide scavenging and reducing power assays were used to evaluate the antioxidant activity.
  
  
  RESULTS
  Alkaloids, phenols, flavonoids, saponins, and terpenes were the main phytochemicals presents in all 21 endophytes. A significant positive correlation was found between antioxidant activity and TPC in fungal extracts. There is 36% endophytic extracts having high phenolic content exhibited potent antioxidant activity. Chaetomium sp., Aspergillus sp., Aspergillus peyronelii and Aspergillus niger strain showed the highest antioxidant activity ranging from 50% to 80% having 58 mg/g to 60 mg/g GAE total phenolics. Ascorbic acid used as a standard showed 90% reducing potential.
  
  
  CONCLUSIONS
  The results reveal that metabolites produced by endophytic fungi isolated from Eugenia jambolana can be a potential source of novel natural antioxidant compounds.
authors.affiliation: Department of Genetics, M.D. University, Rohtak-124001, Haryana, India.
authors.forename: Manila
authors.initials: M
authors.lastname: Yadav
authors.affiliation: Department of Genetics, M.D. University, Rohtak-124001, Haryana, India.
authors.forename: Amita
authors.initials: A
authors.lastname: Yadav
authors.affiliation: Department of Genetics, M.D. University, Rohtak-124001, Haryana, India. Electronic address: yadav1964@rediffmail.com.
authors.forename: Jaya Parkash
authors.initials: JP
authors.lastname: Yadav
keywords: Antioxidant; DPPH; Endophytic fungi; Eugenia jambolana; H(2)O(2); TPC