title: Investigation of association between oipA and iceA1/iceA2 genotypes of Helicobacter pylori and gastric cancer in Iran.
abstract: BACKGROUND
  H pylori is the main causative agent of Gastric cancer and chronic gastritis. Genetic diversity of H. pylori has major contribution in its pathogenesis. We investigated the prevalence of oipA and iceA1/iceA2 positive strains of H. pylori among patients with gastric cancer and gastritis.
  
  
  METHODS
  Sampling performed by means of endoscopy from 86 patients. DNA was extracted from tissue samples using DNA extraction kit. PCR assay was performed and products were monitored by Agarose Gel Electrophoresis.
  
  
  RESULTS
  Urease Test and 16S rRNA PCR did not show significant differences in detection of H. pylori. The frequency of iceA1 allele in patients with gastric cancer was significantly higher than those with gastritis (p<0.05). However, there was no significant difference in prevalence of oipA and iceA2 genes among the two groups of patients (p>0.05).
  
  
  CONCLUSIONS
  The iceA1 gene, but the oipA and iceA2 genes , is associated with H. pylori-induced gastric cancer. However, confirmatory studies must be performed in future.
authors.affiliation: Department of Biology, Pardis International, Guilan University, Guilan, Iran E-mail : safaralizadeh@tabrizu.ac.ir.
authors.forename: Saeed Mahboubi
authors.initials: SM
authors.lastname: Aghdam
authors.forename: Zeinab
authors.initials: Z
authors.lastname: Sardari
authors.forename: Reza
authors.initials: R
authors.lastname: Safaralizadeh
authors.forename: Mortaza
authors.initials: M
authors.lastname: Bonyadi
authors.forename: Reza
authors.initials: R
authors.lastname: Abdolmohammadi
authors.forename: Mostafa Soltani
authors.initials: MS
authors.lastname: Moghadam
authors.forename: Ahad
authors.initials: A
authors.lastname: Khalilnezhad
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics*; D014408:Biomarkers, Tumor / Q000235:genetics*; D005260:Female; D005500:Follow-Up Studies; D005756:Gastritis / Q000235:genetics / Q000473:pathology; D005838:Genotype; D016481:Helicobacter Infections / Q000235:genetics* / Q000473:pathology; D016480:Helicobacter pylori / Q000235:genetics; D006801:Humans; D007492:Iran; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D016133:Polymerase Chain Reaction; D011379:Prognosis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D013274:Stomach Neoplasms / Q000235:genetics* / Q000473:pathology; D014510:Urease / Q000378:metabolism
chemical_list: D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D014408:Biomarkers, Tumor; C408644:IceA2 protein, Helicobacter pylori; C526444:OipA protein, Helicobacter pylori; D012336:RNA, Ribosomal, 16S; C405087:iceA1 protein, Helicobacter pylori; D014510:Urease