title: Protective immunity induced by the vaccination of recombinant Proteus mirabilis OmpA expressed in Pichia pastoris.
abstract: Proteus mirabilis (P. mirabilis) is a zoonotic pathogen that has recently presented a rising infection rate in the poultry industry. To develop an effective vaccine to protect chickens against P. mirabilis infection, OmpA, one of the major outer membrane proteins of P. mirabilis, was expressed in Pichia pastoris. The concentration of the expressed recombinant OmpA protein reached 8.0μg/mL after induction for 96h with 1.0% methanol in the culture. In addition, OmpA protein was confirmed by SDS-PAGE and Western blot analysis using the antibody against Escherichia coli-expressed OmpA protein. Taishan Pinus massoniana pollen polysaccharide, a known plant-derived adjuvant, was mixed into the recombinant OmpA protein to prepare the OmpA subunit vaccine. We then subcutaneously inoculated this vaccine into chickens to examine the immunoprotective effects. ELISA analysis indicated that an excellent antibody response against OmpA was elicited in the vaccinated chickens. Moreover, a high protection rate of 80.0% was observed in the vaccinated group, which was subsequently challenged with P. mirabilis. The results suggest that the eukaryotic P. mirabilis OmpA was an ideal candidate protein for developing an effective subunit vaccine against P. mirabilis infection.
authors.affiliation: Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, College of Animal Science and Technology, Shandong Agricultural University, 61 Daizong Avenue, Taian, Shandong 271018, PR China.
authors.forename: Yongbing
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, College of Animal Science and Technology, Shandong Agricultural University, 61 Daizong Avenue, Taian, Shandong 271018, PR China.
authors.forename: Shifa
authors.initials: S
authors.lastname: Yang
authors.affiliation: Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, College of Animal Science and Technology, Shandong Agricultural University, 61 Daizong Avenue, Taian, Shandong 271018, PR China.
authors.forename: Xiumei
authors.initials: X
authors.lastname: Dai
authors.affiliation: Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, College of Animal Science and Technology, Shandong Agricultural University, 61 Daizong Avenue, Taian, Shandong 271018, PR China.
authors.forename: Liping
authors.initials: L
authors.lastname: Liu
authors.affiliation: Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, College of Animal Science and Technology, Shandong Agricultural University, 61 Daizong Avenue, Taian, Shandong 271018, PR China.
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Jiang
authors.affiliation: Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, College of Animal Science and Technology, Shandong Agricultural University, 61 Daizong Avenue, Taian, Shandong 271018, PR China.
authors.forename: Mingxu
authors.initials: M
authors.lastname: Shao
authors.affiliation: Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, College of Animal Science and Technology, Shandong Agricultural University, 61 Daizong Avenue, Taian, Shandong 271018, PR China.
authors.forename: Shanshan
authors.initials: S
authors.lastname: Chi
authors.affiliation: Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, College of Animal Science and Technology, Shandong Agricultural University, 61 Daizong Avenue, Taian, Shandong 271018, PR China.
authors.forename: Chuanwen
authors.initials: C
authors.lastname: Wang
authors.affiliation: Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, College of Animal Science and Technology, Shandong Agricultural University, 61 Daizong Avenue, Taian, Shandong 271018, PR China.
authors.forename: Cuilian
authors.initials: C
authors.lastname: Yu
authors.affiliation: Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, College of Animal Science and Technology, Shandong Agricultural University, 61 Daizong Avenue, Taian, Shandong 271018, PR China.
authors.forename: Kai
authors.initials: K
authors.lastname: Wei
authors.affiliation: Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, College of Animal Science and Technology, Shandong Agricultural University, 61 Daizong Avenue, Taian, Shandong 271018, PR China. Electronic address: zhangyongbing86@163.com.
authors.forename: Ruiliang
authors.initials: R
authors.lastname: Zhu
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification / Q000378:metabolism; D001428:Bacterial Vaccines / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D002645:Chickens; D010843:Pichia / Q000235:genetics / Q000378:metabolism; D011512:Proteus Infections / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control; D011513:Proteus mirabilis / Q000235:genetics; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification / Q000378:metabolism; D022223:Vaccines, Subunit / Q000235:genetics / Q000276:immunology* / Q000378:metabolism
chemical_list: D000907:Antibodies, Bacterial; D001425:Bacterial Outer Membrane Proteins; D001428:Bacterial Vaccines; D011994:Recombinant Proteins; D022223:Vaccines, Subunit; C078167:OMPA outer membrane proteins
keywords: Eukaryotic expression; OmpA; Pichia pastoris; Proteus mirabilis; Subunit vaccine