title: [CD147 expression level and rs8259 T/A polymorphism of CD147 in patients with acute coronary syndrome].
abstract: OBJECTIVE
  To investigate the association between CD147 expression and its untranslated regions 3'UTR rs8259 T/A polymorphism and acute coronary syndrome (ACS).
  
  
  METHODS
  The genotypes of CD147 were detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) methods in 182 ACS patients and 328 healthy controls. The plasma level of CD147 was determined by enzyme-linked immunosorbent assay (ELISA). CD147 mRNA and protein expression was detected by real-time fluorescent quantitative PCR (RT-qPCR) and Western blot.
  
  
  RESULTS
  The plasma CD147 level obtained from radial artery in ACS patients ((3.63 ± 0.70) pg/L) was significantly higher than in control ((2.45 ± 0.27) pg/L, P < 0.05), and highest in plasma obtained from the coronary artery ((4.28 ± 1.03) pg/L, P < 0.05) in ACS patients. Furthermore, the plasma CD147 level was higher in the ACS patients with rs8259 AA genotype than in the ACS patients with rs8259 TT genotype ((4.08 ± 0.41) pg/L vs. (3.05 ± 0.79) pg/L in radial artery and (5.29 ± 0.62) pg/L vs. (3.13 ± 0.52) pg/L in coronary artery, both P < 0.05). There are an enhanced expression of CD147 mRNA (2.45 times higher than control) and protein (3.66 ± 1.56 vs. 1.81 ± 1.29) in PBMCs from ACS patients than that from controls (both P < 0.05). The PBMCs CD147 mRNA and protein expression level were significantly higher in ACS patients with rs8259 AA genotype (mRNA:2.45 ± 0.35, protein:1.63 ± 0.16) compared to ACS patients with rs8259 TT genotype (mRNA:1.69 ± 0.15, protein: 0.88 ± 0.16, both P < 0.05). Multiple logistic analysis showed that CD147 T allele (AT+TT) was a protective factor to ACS (OR = 0.667, 95% CI 0.507-0.879, P < 0.05).
  
  
  CONCLUSIONS
  The over-expression of CD147 is involved in the pathogenesis of ACS. The CD147 3'UTR rs8259 T allele may be a protective factor for ACS, its polymorphism can affect the CD147 protein expression in ACS patients.
authors.affiliation: Department of Cardiology, Affiliated Hospital of Jiangsu University,Zhenjiang 212001, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Mao
authors.affiliation: Department of Cardiology, Affiliated Hospital of Jiangsu University,Zhenjiang 212001, China. Email: yanjinchuan@hotmail.com.
authors.forename: Jinchuan
authors.initials: J
authors.lastname: Yan
authors.affiliation: Department of Cardiology, Affiliated Hospital of Jiangsu University,Zhenjiang 212001, China.
authors.forename: Cuiping
authors.initials: C
authors.lastname: Wang
authors.affiliation: Department of Cardiology, Affiliated Hospital of Jiangsu University,Zhenjiang 212001, China.
authors.forename: Zhongqun
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Department of Cardiology, Affiliated Hospital of Jiangsu University,Zhenjiang 212001, China.
authors.forename: Peijing
authors.initials: P
authors.lastname: Liu
authors.affiliation: Department of Cardiology, Affiliated Hospital of Jiangsu University,Zhenjiang 212001, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Yuan
mesh_terms: D054058:Acute Coronary Syndrome / Q000235:genetics*; D000483:Alleles; D051926:Basigin / Q000096:biosynthesis* / Q000235:genetics; D016022:Case-Control Studies; D005838:Genotype; D006801:Humans; D007963:Leukocytes, Mononuclear; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length
chemical_list: C497886:BSG protein, human; D051926:Basigin