title: Dynamic nuclear polarization-enhanced NMR on aligned lipid bilayers at ambient temperature.
abstract: Dynamic nuclear polarization (DNP)-enhanced solid-state NMR spectroscopy has been shown to hold great potential for functional studies of membrane proteins at low temperatures due to its great sensitivity improvement. There are, however, numerous applications for which experiments at ambient temperature are desirable and which would also benefit from DNP signal enhancement. Here, we demonstrate as a proof of concept that a significant signal increase for lipid bilayers under room-temperature conditions can be achieved by utilizing the Overhauser effect. Experiments were carried out on aligned bilayers at 400 MHz/263 GHz using a stripline structure combined with a Fabry-Perot microwave resonator. A signal enhancement of protons of up to -10 was observed. Our results demonstrate that Overhauser DNP at high field provides efficient polarization transfer within insoluble samples, which is driven by fast local molecular fluctuations. Furthermore, our experimental setup offers an attractive option for DNP-enhanced solid-state NMR on ordered membranes and provides a general perspective toward DNP at ambient temperatures.
authors.affiliation: Institute of Biophysical Chemistry and ‡Institute of Physical and Theoretical Chemistry, Center for Biomolecular Magnetic Resonance Frankfurt, Goethe University Frankfurt , 60438 Frankfurt am Main, Germany.
authors.forename: Orawan
authors.initials: O
authors.lastname: Jakdetchai
authors.forename: Vasyl
authors.initials: V
authors.lastname: Denysenkov
authors.forename: Johanna
authors.initials: J
authors.lastname: Becker-Baldus
authors.forename: Bercem
authors.initials: B
authors.lastname: Dutagaci
authors.forename: Thomas F
authors.initials: TF
authors.lastname: Prisner
authors.forename: Clemens
authors.initials: C
authors.lastname: Glaubitz
mesh_terms: D008051:Lipid Bilayers / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D013696:Temperature
chemical_list: D008051:Lipid Bilayers