title: Investigation of flurbiprofen genotoxicity and cytotoxicity in rat bone marrow cells.
abstract: This study was performed to investigate cytogenetic effects of NSAID flurbiprofen which was used as active ingredient in some analgesic, antipyretic and anti-inflammatory drugs. Genotoxic effect of flurbiprofen was investigated using in vivo chromosome aberration (CA) test and random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) test. Also, oxidative stress potential of flurbiprofen was determined by measuring total oxidant and antioxidant level which occurred with flurbiprofen treatment in rat peripheral blood. For these purposes, rats were treated with three concentrations of flurbiprofen (29.25, 58.50 and 117 mg/kg, body weight) in single dose at two different treatment periods (12 and 24 h). According to the results, flurbiprofen did not affect chromosome aberrations in rat bone marrow cells with CA test. In RAPD-PCR test, polymorphic bands were unaffected. Also, test substance did not change total oxidant and antioxidant status (except for 58.50 and 117 mg/kg, 12 h) and therefore it did not lead to significant increase on oxidative stress (again except 58.50 and 117 mg/kg, 12 h). However, flurbiprofen reduced to mitotic indexes and these reductions were dose-dependent for 12 h treatment. In summary, flurbiprofen did not show significant genotoxic effect. But it caused cytotoxicity in rat bone marrow cells.
authors.affiliation: Department of Biology, Natural and Applied Science Institute, Cukurova University , Adana , Turkey and.
authors.forename: Taygun
authors.initials: T
authors.lastname: Timocin
authors.forename: Hasan B
authors.initials: HB
authors.lastname: Ila
mesh_terms: D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000633:toxicity*; D000975:Antioxidants / Q000378:metabolism; D001854:Bone Marrow Cells / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D049109:Cell Proliferation / Q000187:drug effects*; D002869:Chromosome Aberrations / Q000139:chemically induced; D004249:DNA Damage; D004305:Dose-Response Relationship, Drug; D005260:Female; D005480:Flurbiprofen / Q000633:toxicity*; D008297:Male; D008938:Mitosis / Q000187:drug effects; D008940:Mitotic Index; D018384:Oxidative Stress / Q000187:drug effects; D016133:Polymerase Chain Reaction; D017207:Rats, Sprague-Dawley; D018570:Risk Assessment; D013997:Time Factors
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D000975:Antioxidants; D005480:Flurbiprofen
keywords: Cytotoxicity; RAPD; flurbiprofen; genotoxicity; oxidative stress; rat bone marrow