title: Integrated Design of Antibodies for Systems Biology UsingAb Designer.
abstract: In the current era of large-scale biology, systems biology has evolved as a powerful approach to identify complex interactions within biological systems. In addition to high throughput identification and quantification techniques, methods based on high-quality mono-specific antibodies remain an essential element of the approach. To assist the large-scale design and production of peptide-directed antibodies for systems biology studies, we developed a fully integrated online application,AbDesigner(http://helixweb.nih.gov/AbDesigner/), to help researchers select optimal peptide immunogens for antibody generation against relatively disordered regions of target proteins. Here we describeAbDesignerin terms of its features, comparing it to other software tools, and use it to design three antibodies against kidney disease-related proteins in human, viz. nephrin, podocin, and apolipoprotein L1.
authors.affiliation: Epithelial Systems Biology Laboratory, National Heart, Lung and Blood Institute, NIH, Bethesda, MD, USA ; Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand.
authors.forename: Trairak
authors.initials: T
authors.lastname: Pisitkun
authors.affiliation: Kidney Disease Section, National Institute of Diabetes and Digestive and Kidney Diseases, NIH, Bethesda, MD, USA.
authors.forename: Patrick
authors.initials: P
authors.lastname: Dummer
authors.affiliation: Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand.
authors.forename: Poorichaya
authors.initials: P
authors.lastname: Somparn
authors.affiliation: Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand.
authors.forename: Nattiya
authors.initials: N
authors.lastname: Hirankarn
authors.affiliation: Kidney Disease Section, National Institute of Diabetes and Digestive and Kidney Diseases, NIH, Bethesda, MD, USA.
authors.forename: Jeffrey B
authors.initials: JB
authors.lastname: Kopp
authors.affiliation: Epithelial Systems Biology Laboratory, National Heart, Lung and Blood Institute, NIH, Bethesda, MD, USA.
authors.forename: Mark A
authors.initials: MA
authors.lastname: Knepper
keywords: Antibody design; Software tools; Systems biology