title: In vitro enhancement of psoralen as an important anticancer compound in Psoralea corylifolia through precursor feeding.
abstract: BACKGROUND
  Psoralea corylifolia L. (Fabacese) is rich source of bioactive compounds, which endows the plant with immense value for its use in pharmaceuticals, health, and body-care products.
  
  
  OBJECTIVE
  The current study was designed (i) for the determination of psoralen from callus derived from different plant parts, and (ii) for the enhancement of psoralen in in vitro condition with the treatment of various psoralen pathway precursors.
  
  
  METHODS
  B5 media were employed for raising the cultures from different plant parts such as leaf, node, root, and green seeds. Cotyledons' calluses were derived from cotyledon of green seeds that were elicited on MS + 10 µM BA + 5 µM IBA medium supplemented at 0.1, 1, 2.5, 5, 25, and 50 mg/L of various psoralen pathway precursors such as umbelliferone, cinnamic acid, and NADPH. The method for extraction of psoralen was modified from the Singh method and the content of psoralen was measured using HPLC.
  
  
  RESULTS
  HPLC analysis of callus derived from different parts of P. corylifolia revealed that a maximum of psoralen (2601.8 µg/g fresh wt.) was recorded in cotyledons' callus. Cotyledonary callus was chosen for the enhancement of psoralens because of higher amount of psoralen in it. In vitro evaluation showed that all the precursors enhanced the psoralen amount dramatically so that the optimum amount of psoralen (2518.8 µg/g fresh wt.) was obtained at 2.5 mg/L cinnamic acid.
  
  
  CONCLUSIONS
  The results obtained indicate that cinnamic acid is one of the important precursors of psoralen pathway that induced a maximum amount of psoralen with in vitro conditions.
authors.affiliation: Department of Biology, Faculty of Science, University of Malayer , Malayer , Iran .
authors.forename: Behrooz
authors.initials: B
authors.lastname: Mohammadparast
authors.forename: Ali Reza
authors.initials: AR
authors.lastname: Rustaiee
authors.forename: Mousa
authors.initials: M
authors.lastname: Rasouli
authors.forename: Sannaz
authors.initials: S
authors.lastname: Zardari
authors.forename: Veena
authors.initials: V
authors.lastname: Agrawal
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000302:isolation & purification*; D003470:Culture Media / Q000494:pharmacology; D005363:Ficusin / Q000302:isolation & purification*; D010936:Plant Extracts / Q000302:isolation & purification*; D018515:Plant Leaves / Q000187:drug effects; D018517:Plant Roots / Q000187:drug effects; D029907:Psoralea / Q000187:drug effects; D012639:Seeds / Q000187:drug effects
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D003470:Culture Media; D010936:Plant Extracts; D005363:Ficusin
keywords: Anticancerous; biosynthesis pathway; cinnamic acid; medicinal plants; umbelliferone