title: Major proteomic changes associated with amyloid-induced biofilm formation in Pseudomonas aeruginosa PAO1.
abstract: The newly identified functional amyloids in Pseudomonas (Fap) are associated with increased aggregation and biofilm formation in the opportunistic pathogen P. aeruginosa; however, whether this phenomenon can be simply ascribed to the mechanical properties of the amyloid fibrils remains undetermined. To gain a deeper understanding of the Fap-mediated biofilm formation, the physiological consequences of Fap expression were investigated using label-free protein quantification. The functional amyloids were found to not solely act as inert structural biofilm components. Their presence induced major changes in the global proteome of the bacterium. These included the lowered abundance of classical virulence factors such as elastase B and the secretion system of alkaline protease A. Amyloid-mediated biofilm formation furthermore increased abundance of the alginate and pyoverdine synthesis machinery, which turned P. aeruginosa PAO1 into an unexpected mucoid phenotype. The results imply a significant impact of functional amyloids on the physiology of P. aeruginosa with subsequent implications for biofilm formation and chronic infections.
authors.affiliation: Center for Microbial Communities; ‡Department of Biotechnology, Chemistry, and Environmental Engineering; and §Department of Health Science and Technology, Aalborg University , DK-9000 Aalborg, Denmark.
authors.forename: Florian-Alexander
authors.initials: FA
authors.lastname: Herbst
authors.forename: Mads T
authors.initials: MT
authors.lastname: Søndergaard
authors.forename: Henrik
authors.initials: H
authors.lastname: Kjeldal
authors.forename: Allan
authors.initials: A
authors.lastname: Stensballe
authors.forename: Per H
authors.initials: PH
authors.lastname: Nielsen
authors.forename: Morten S
authors.initials: MS
authors.lastname: Dueholm
mesh_terms: D000682:Amyloid / Q000502:physiology*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D018441:Biofilms; D006801:Humans; D014176:Protein Biosynthesis; D040901:Proteomics; D011550:Pseudomonas aeruginosa / Q000502:physiology*
chemical_list: D000682:Amyloid; D001426:Bacterial Proteins
keywords: Pseudomonas aeruginosa PAO1; biofilms; chronic infection; cystic fibrosis; functional amyloids; microbial proteomics