title: The assembly and use of tethered bilayer lipid membranes (tBLMs).
abstract: Because they are firmly held in place, tethered bilayer lipid membranes (tBLMs) are considerably more robust than supported lipid bilayers such as black lipid membranes (BLMs) (Cornell et al. Nature 387(6633): 580-583, 1997). Here we describe the procedures required to assemble and test tethered lipid bilayers that can incorporate various lipid species, peptides, and ion channel proteins.
authors.affiliation: Victor Chang Cardiac Research Institute, Darlinghurst, NSW, 2010, Australia, c.cranfield@victorchang.edu.au.
authors.forename: Charles
authors.initials: C
authors.lastname: Cranfield
authors.forename: Sonia
authors.initials: S
authors.lastname: Carne
authors.forename: Boris
authors.initials: B
authors.lastname: Martinac
authors.forename: Bruce
authors.initials: B
authors.lastname: Cornell
mesh_terms: D002462:Cell Membrane; D058266:Dielectric Spectroscopy; D004220:Disulfides / Q000737:chemistry; D004566:Electrodes; D008051:Lipid Bilayers / Q000737:chemistry; D008967:Molecular Biology / Q000295:instrumentation / Q000379:methods*; D010455:Peptides / Q000737:chemistry
chemical_list: D004220:Disulfides; D008051:Lipid Bilayers; D010455:Peptides