title: Increased phosphorylation of the RcsB regulator of the RcsCDB phosphorelay in strains of Dickeya dadantii devoid of osmoregulated periplasmic glucans revealed by Phos-tag gel analysis.
abstract: Osmoregulated periplasmic glucans (OPGs) are general constituents of many proteobacteria. OPGs are important factors required for full virulence in many pathogens including Dickeya dadantii. D. dadantii causes the soft-rot disease in a wide range of plant species. The pleiotropic phenotype of opg-negative strains includes total loss of virulence and motility, and is linked to the constitutive activation of the RcsCDB phosphorelay, deduced from expression analysis of genes of the RcsCDB regulon. The constitutive activation of the RcsCDB phosphorelay in an opg-negative strain was demonstrated by direct analysis of the phosphorylation level of the RcsB regulator protein in vivo by using a Phos-tag retardation gel approach, and was correlated with the phenotype and the expression of motility genes. Data revealed a low level of RcsB phosphorylated form in the wild-type strain and a slight increase of phosphorylation in opgG mutant strains sufficient to induce the pleiotropic phenotype observed.
authors.affiliation: Unité de Glycobiologie Structurale et Fonctionnelle, UMR 8576 Lille 1-CNRS, Université des Sciences et Technologies de Lille, Université Lille - Nord de France, 59655 Villeneuve d'Ascq, France.
authors.forename: Edwige
authors.initials: E
authors.lastname: Madec
authors.affiliation: Unité de Glycobiologie Structurale et Fonctionnelle, UMR 8576 Lille 1-CNRS, Université des Sciences et Technologies de Lille, Université Lille - Nord de France, 59655 Villeneuve d'Ascq, France.
authors.forename: Sébastien
authors.initials: S
authors.lastname: Bontemps-Gallo
authors.affiliation: Unité de Glycobiologie Structurale et Fonctionnelle, UMR 8576 Lille 1-CNRS, Université des Sciences et Technologies de Lille, Université Lille - Nord de France, 59655 Villeneuve d'Ascq, France.
authors.forename: Jean-Marie
authors.initials: JM
authors.lastname: Lacroix
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism*; D004586:Electrophoresis; D004755:Enterobacteriaceae / Q000235:genetics / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D010766:Phosphorylation; D011499:Protein Processing, Post-Translational
chemical_list: D001426:Bacterial Proteins; C074889:RcsB protein, Bacteria