title: Versatile cellular uptake mediated by catanionic vesicles: simultaneous spontaneous membrane fusion and endocytosis.
abstract: Lactose-derived catanionic vesicles offer unique opportunities to overcome cellular barriers. These potential nanovectors, very easy to formulate as drug delivery systems, are able to encapsulate drugs of various hydrophilicity. This article highlights versatile interaction mechanisms between these catanionic vesicles, labeled with hydrophilic and amphiphilic fluorescent probes, and a mammalian cell line, Chinese Hamster Ovary. Confocal microscopy and flow cytometry techniques show that these vesicles are internalized by cells through cellular energy dependent processes, as endocytosis, but are simultaneously able to spontaneously fuse with cell plasma membranes and release their hydrophilic content directly inside the cytosol. Such innovative and polyvalent nanovectors, able to deliver their content via different internalization pathways, would positively be a great progress for the coadministration of drugs of complementary efficiency.
authors.affiliation: Laboratoire des Interactions Moléculaires et Réactivité Chimique et Photochimique, UMR 5623 Université Paul Sabatier and Centre National de la Recherche Scientifique , 118 route de Narbonne, 31062 Toulouse, France.
authors.forename: Chloé
authors.initials: C
authors.lastname: Mauroy
authors.forename: Pauline
authors.initials: P
authors.lastname: Castagnos
authors.forename: Julie
authors.initials: J
authors.lastname: Orio
authors.forename: Marie-Claire
authors.initials: MC
authors.lastname: Blache
authors.forename: Isabelle
authors.initials: I
authors.lastname: Rico-Lattes
authors.forename: Justin
authors.initials: J
authors.lastname: Teissié
authors.forename: Marie-Pierre
authors.initials: MP
authors.lastname: Rols
authors.forename: Muriel
authors.initials: M
authors.lastname: Blanzat
mesh_terms: D000818:Animals; D016466:CHO Cells; D002412:Cations; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D002469:Cell Separation; D003412:Cricetulus; D003600:Cytosol; D016503:Drug Delivery Systems; D004705:Endocytosis; D005434:Flow Cytometry; D005456:Fluorescent Dyes / Q000737:chemistry; D006017:Glycolipids / Q000737:chemistry; D007700:Kinetics; D007785:Lactose / Q000737:chemistry; D008561:Membrane Fusion; D008567:Membranes, Artificial; D018613:Microscopy, Confocal; D013501:Surface-Active Agents
chemical_list: D002412:Cations; D005456:Fluorescent Dyes; D006017:Glycolipids; D008567:Membranes, Artificial; D013501:Surface-Active Agents; D007785:Lactose
keywords: catanionic vesicles; drug delivery; endocytosis; glycolipids; membrane fusion