title: Mechanisms of improvement of intestinal transport of baicalin and puerarin by extracts of Radix Angelicae Dahuricae.
abstract: Radix Angelicae Dahuricae is the dried root of Angelicae Dahurica (Fisch.ex Hoffm.)Benth.et Hook.f. var.formosana (Boiss.) Shan et Yuan (Fam.Umbelliferae). The total coumarins (Cou) and volatile oil (VO) were main active components that drived from Radix Angelicae Dahuricae. Our previous studies have shown that Cou and VO could increase intestinal absorption for transmucosal drug delivery with unknown mechanism. The aim of this study was to investigate the molecular mechanism of Radix Angelicae Dahuricae for improving drug intestinal transport. Caco-2 cell model was used to study the effect of Radix Angelicae Dahurica on transepithelial electrical resistance. Western blot was used to study its effect on the expression of the actin and ZO-1, tight junction proteins. The effect of Radix Angelicae Dahurica on the expression of P-gp protein was investigated using flow cytometry. VO (0.036-2.88 μL/mL) and Cou (0.027-0.54 mg/mL) caused a reversible, time- and dose-dependent decrease in transepithelial electrical resistance. VO and/or Cou could inhibit the expression of the tight junction protein, ZO-1 and actin. VO and/or Cou also could inhibit the expression of P-gp. These data suggested that Radix Angelicae Dahurica increased cell permeability by affecting the expression of actin, ZO-1 or P-gp, opening the tight junction or inhibiting the efflux induced by P-gp.
authors.affiliation: Key Laboratory of Modern Preparation of TCM of Ministry of Education, Jiangxi University of TCM, Nanchang, 330004, PR China.
authors.forename: Xin-Li
authors.initials: XL
authors.lastname: Liang
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.forename: Guo-Wei
authors.initials: GW
authors.lastname: Zhao
authors.forename: Zhe
authors.initials: Z
authors.lastname: Li
authors.forename: Yun
authors.initials: Y
authors.lastname: Luo
authors.forename: Zheng-Gen
authors.initials: ZG
authors.lastname: Liao
authors.forename: Dong-Mei
authors.initials: DM
authors.lastname: Yan
mesh_terms: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000378:metabolism; D000199:Actins / Q000378:metabolism; D029969:Angelica / Q000737:chemistry*; D001692:Biological Transport / Q000187:drug effects; D018938:Caco-2 Cells; D003374:Coumarins / Q000494:pharmacology; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005419:Flavonoids / Q000493:pharmacokinetics*; D006801:Humans; D007408:Intestinal Absorption / Q000187:drug effects*; D007413:Intestinal Mucosa / Q000378:metabolism; D007529:Isoflavones / Q000493:pharmacokinetics*; D009822:Oils, Volatile / Q000494:pharmacology; D018517:Plant Roots / Q000737:chemistry; D019108:Tight Junctions / Q000187:drug effects; D062826:Zonula Occludens-1 Protein / Q000378:metabolism
chemical_list: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D000199:Actins; D003374:Coumarins; D004365:Drugs, Chinese Herbal; D005419:Flavonoids; D007529:Isoflavones; D009822:Oils, Volatile; C569042:TJP1 protein, human; D062826:Zonula Occludens-1 Protein; C038044:baicalin; C033607:puerarin
keywords: Caco-2 cells; P-gp; Radix Angelicae Dahuricae; molecular mechanisms; tight junctions