title: Polygonum cuspidatum extracts as bioactive antioxidaion, anti-tyrosinase, immune stimulation and anticancer agents.
abstract: In our study, it was applied for the technology of supercritical fluid carbon dioxide extraction to achieve biological constitutes from a Taiwan native plant, Polygonum cuspidatum. We developed bioactive effects of P. cuspidatum extracts via multiple examinations that established bio-purposes at a range of dosage ranges. The research of P. cuspidatum extracts indicated that they possessed anti-oxidative properties on radical-scavenging abilities, reducing activities and metal chelating powers in dose-dependant manners. The extracts also had minor in vitro mushroom tyrosinase suppression and decreased cellular tyrosinase activities and melanin production in B16-F10 cells. Immunologically, P. cuspidatum extracts enhanced the release of tumor necrosis factor α (TNF-α) induced by THP-1 macrophage cell line. In addition, the cell proliferation showed anti-proliferation in dose-dependent manner on human skin melanoma cells, A375 and A375.S2, of the extracts suggesting biological constitutes employed the anti-cancer possessions. This is the first statement presenting bioactivities on P. cuspidatum extracts including anti-oxidation, immune stimulation, anti-tyrosinase and anti-melanoma as far as we know.
authors.affiliation: Department of Chemical Engineering, National Chung Hsing University, Taichung, Taiwan, ROC.
authors.forename: Chih-Chen
authors.initials: CC
authors.lastname: Lee
authors.affiliation: Department of Fragrance and Cosmetic Science, Kaohsiung Medical University, Kaohsiung, Taiwan, ROC.
authors.forename: Yen-Ting
authors.initials: YT
authors.lastname: Chen
authors.affiliation: Department of Biotechnology, Kaohsiung Medical University, Kaohsiung, Taiwan, ROC.
authors.forename: Chien-Chih
authors.initials: CC
authors.lastname: Chiu
authors.affiliation: Department of Biotechnology, Kaohsiung Medical University, Kaohsiung, Taiwan, ROC.
authors.forename: Wei-Ting
authors.initials: WT
authors.lastname: Liao
authors.affiliation: Department of Chemical Engineering, National Chung Hsing University, Taichung, Taiwan, ROC.
authors.forename: Yung-Chuan
authors.initials: YC
authors.lastname: Liu
authors.affiliation: Department of Fragrance and Cosmetic Science, Kaohsiung Medical University, Kaohsiung, Taiwan, ROC; Graduate Institute of Natural Products, Kaohsiung Medical University, Kaohsiung, Taiwan, ROC. Electronic address: davidw@kmu.edu.tw.
authors.forename: Hui-Min
authors.initials: HM
authors.lastname: David Wang
mesh_terms: D000363:Agaricales / Q000201:enzymology; D000970:Antineoplastic Agents / Q000494:pharmacology*; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D025924:Chromatography, Supercritical Fluid; D045468:Fallopia japonica / Q000737:chemistry*; D006801:Humans; D007109:Immunity / Q000187:drug effects* / Q000276:immunology; D008264:Macrophages / Q000187:drug effects / Q000276:immunology; D008543:Melanins / Q000096:biosynthesis; D008545:Melanoma / Q000188:drug therapy / Q000473:pathology*; D014442:Monophenol Monooxygenase / Q000037:antagonists & inhibitors* / Q000378:metabolism; D010084:Oxidation-Reduction / Q000187:drug effects; D010936:Plant Extracts / Q000494:pharmacology*; D013624:Taiwan
chemical_list: D000970:Antineoplastic Agents; D008543:Melanins; D010936:Plant Extracts; D014442:Monophenol Monooxygenase
keywords: Anti-cancer; Anti-tyrosinase; Antioxidant; Immune; Polygonum cuspidatum; Supercritical fluid carbon dioxide extraction