title: [Evaluation of c-myc and CCNE2 amplification in breast cancer with quantitative multi-gene fluorescence in-situ hybridization].
abstract: OBJECTIVE
  To investigate c-myc and CCNE2 gene amplifications and their relationship in breast cancer.
  
  
  METHODS
  Sixty-six infiltrating ductal breast carcinomas with foci of ductal carcinoma in situ components collected from January 2005 to December 2007 were selected for tissue microarray and quantitative multi-gene FISH for c-myc and CCNE2 gene amplification, and the relationship with the clinicopathologic features was analyzed.
  
  
  RESULTS
  Of the 66 cases, 18 (27.3%) showed c-myc amplification and 23 (34.8%) showed CCNE2 amplification. A strong correlation was found between c-myc and CCNE2 amplification (P < 0.01). The breast cancers showing c-myc and CCNE2 amplifications were all aneuploidy, and were HER2 positive (P < 0.05). Tumors with c-myc amplification also showed higher Ki-67 index (P < 0.05).
  
  
  CONCLUSIONS
  C-myc and CCNE2 amplifications are common events in breast cancer, and they often coexist. C-myc and CCNE2 genes may play critical roles in the pathogenesis and development of breast cancer through unique and overlapping signaling pathways.
authors.affiliation: Department of Pathology, Shandong University School of Medicine, Jinan 250012, China.
authors.forename: Zhishuang
authors.initials: Z
authors.lastname: Li
authors.forename: Qingyong
authors.initials: Q
authors.lastname: Meng
authors.forename: Qiong
authors.initials: Q
authors.lastname: Yu
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Zhou
authors.affiliation: E-mail: lillie6636@sdu.edu.cn.
authors.forename: Li
authors.initials: L
authors.lastname: Li
mesh_terms: D000782:Aneuploidy; D001943:Breast Neoplasms / Q000235:genetics*; D002278:Carcinoma in Situ / Q000235:genetics*; D018270:Carcinoma, Ductal, Breast / Q000235:genetics*; D002285:Carcinoma, Intraductal, Noninfiltrating / Q000235:genetics*; D016213:Cyclins / Q000235:genetics*; D005260:Female; D005784:Gene Amplification; D016259:Genes, myc; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods; D046888:Tissue Array Analysis
chemical_list: C503688:CCNE2 protein, human; D016213:Cyclins