title: Distribution of high-risk human papillomavirus genotypes in HPV-infected women in Beijing, China.
abstract: To investigate the distribution of high-risk human papillomavirus (HPV) genotypes in infected women from Beijing, China, samples were obtained during routine gynecologic examinations and DNA was extracted from the samples, and PCR was performed to distinguish the 13 high-risk HPV subtypes (16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, and 68). Samples were obtained from 1160 women (20-75 years old), and 470 cases of high-risk HPV infection were diagnosed. HPV 52, HPV 16, HPV 58, HPV 51, and HPV 39 were the most common genotypes accounting for 22.8%, 22.3%, 20.0%, 14.3%, and 13.6% of cases, respectively. The highest infection rates were found in 20-30 year-old patients (35.1%). HPV 16 infection was the highest in the 31-40 year-old group, and HPV 52, HPV 58, and HPV 39 infections were highest in the 20-30 year-old group. Some patients were infected with multiple high-risk HPV subtypes. Of the 470 patients with positive HPV tests, 65.7% of women were infected with a single high-risk HPV subtype, 23.2%, of women were infected with two high-risk HPV subtypes, 7.7% were infected with three subtypes, and 3.4% of women were infected with more than three high-risk HPV subtypes. In this study, HPV 16 and HPV 52 were the most common subtypes found in patients with cervical lesions.
authors.affiliation: Molecular Diagnostics Center, Clinical Laboratory, China-Japan Friendship Hospital, Beijing, China.
authors.forename: Shuang
authors.initials: S
authors.lastname: Lu
authors.forename: Xiao
authors.initials: X
authors.lastname: Cong
authors.forename: Min
authors.initials: M
authors.lastname: Li
authors.forename: FengXia
authors.initials: F
authors.lastname: Chang
authors.forename: Liang
authors.initials: L
authors.lastname: Ma
authors.forename: Yong Tong
authors.initials: YT
authors.lastname: Cao
mesh_terms: D000328:Adult; D000367:Age Factors; D002584:Cervix Uteri / Q000821:virology*; D002681:China / Q000453:epidemiology; D060085:Coinfection / Q000453:epidemiology / Q000821:virology; D004279:DNA, Viral / Q000235:genetics / Q000302:isolation & purification; D005260:Female; D005838:Genotype; D006801:Humans; D008875:Middle Aged; D027383:Papillomaviridae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D030361:Papillomavirus Infections / Q000453:epidemiology* / Q000821:virology*; D016133:Polymerase Chain Reaction; D015995:Prevalence; D012189:Retrospective Studies; D055815:Young Adult
chemical_list: D004279:DNA, Viral
keywords: Beijing area; genotypes; high-risk human papillomavirus (HPV)