title: Blood pressure levels in male carriers of Arg82Cys in CD300LG.
abstract: The genetics of hypertension has been scrutinized in large-scale genome-wide association studies (GWAS) with a large number of common genetic variants identified, each exerting subtle effects on disease susceptibility. An amino acid polymorphism, p.Arg82Cys, in CD300LG was recently found to be associated with fasting HDL-cholesterol and triglyceride levels. The polymorphism has not been detected in hypertension GWAS potentially due to its low frequency, but CD300LG has been linked to blood pressure as CD300LG knockout mice have changes in blood pressure. Twenty-four-hour ambulatory blood pressure was obtained in human CD300LG CT-carriers to follow up on these observations.
  
  
  METHODS
  Twenty healthy male CD300LG rs72836561 CT-carriers matched for age and BMI with 20 healthy male CC-carriers. Office blood pressure, 24-hour ambulatory blood pressure, carotid intima-media thickness (CIMT), and fasting blood samples were evaluated. The clinical study was combined with a genetic-epidemiological study to replicate the association between blood pressure and CD300LG Arg82Cys in 2,637 men and 3,249 women.
  
  
  RESULTS
  CT-carriers had a higher 24-hour ambulatory systolic blood pressure (122 mmHg versus 115; p = 0.01) and diastolic blood pressure (77 mmHg versus 72; p<0.01) compared with CC-carriers. There were no differences in CIMT between the two groups. Metalloproteinase-9 level was higher in CT-carriers than in CC-carriers (P<0.01). However, no association between office blood pressure and CD300LG genotype was detected in the genetic-epidemiological study.
  
  
  CONCLUSIONS
  Although 24-hour blood pressure, measured with a sensitive method, in a small sample of CD300LG rs72836561 CT-carriers was higher than in CC-carriers, this did not translate into significant differences in office blood pressure in a larger cohort. This discrepancy which may reflect differences in methodological approach, underlines the importance of performing replication studies in a larger clinical context, but a formal rejection of a relation between blood pressure and CD300LG requires measurement of 24-hour ambulatory blood pressure in a larger cohort.
authors.affiliation: Department of Internal Medicine and Endocrinology, Aarhus University Hospital, Aarhus, Denmark.
authors.forename: Julie
authors.initials: J
authors.lastname: Støy
authors.affiliation: The Novo Nordisk Foundation Center for Basic Metabolic Research, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark.
authors.forename: Niels
authors.initials: N
authors.lastname: Grarup
authors.affiliation: Department of Radiology, Aarhus University Hospital, Aarhus, Denmark.
authors.forename: Arne
authors.initials: A
authors.lastname: Hørlyck
authors.affiliation: Department of Radiology, Aarhus University Hospital, Aarhus, Denmark.
authors.forename: Liselotte
authors.initials: L
authors.lastname: Ibsen
authors.affiliation: Department of Internal Medicine and Endocrinology, Aarhus University Hospital, Aarhus, Denmark; Department of Endocrinology, Rigshospitalet, Copenhagen, Denmark.
authors.forename: Jørgen
authors.initials: J
authors.lastname: Rungby
authors.affiliation: Department of Internal Medicine and Endocrinology, Aarhus University Hospital, Aarhus, Denmark.
authors.forename: Per Løgstrup
authors.initials: PL
authors.lastname: Poulsen
authors.affiliation: Department of Clinical Biochemistry, Vejle Hospital, Vejle, Denmark.
authors.forename: Ivan
authors.initials: I
authors.lastname: Brandslund
authors.affiliation: Department of Medicine, Vejle Hospital, Vejle, Denmark.
authors.forename: Cramer
authors.initials: C
authors.lastname: Christensen
authors.affiliation: The Novo Nordisk Foundation Center for Basic Metabolic Research, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark.
authors.forename: Torben
authors.initials: T
authors.lastname: Hansen
authors.affiliation: The Novo Nordisk Foundation Center for Basic Metabolic Research, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark.
authors.forename: Oluf
authors.initials: O
authors.lastname: Pedersen
authors.affiliation: Department of Internal Medicine and Endocrinology, Aarhus University Hospital, Aarhus, Denmark; Medical Research Laboratories, Institute for Clinical Medicine, Aarhus University, Aarhus, Denmark.
authors.forename: Niels
authors.initials: N
authors.lastname: Møller
authors.affiliation: Department of Internal Medicine and Endocrinology, Aarhus University Hospital, Aarhus, Denmark.
authors.forename: Ulla
authors.initials: U
authors.lastname: Kampmann
mesh_terms: D000328:Adult; D000368:Aged; D015703:Antigens, CD / Q000235:genetics*; D001794:Blood Pressure / Q000235:genetics; D059168:Carotid Intima-Media Thickness; D016022:Case-Control Studies; D005260:Female; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D006579:Heterozygote; D006801:Humans; D006973:Hypertension / Q000235:genetics*; D008297:Male; D020125:Mutation, Missense; D011971:Receptors, Immunologic / Q000235:genetics*
chemical_list: D015703:Antigens, CD; C584286:CD300LG protein, human; D011971:Receptors, Immunologic