title: Periconceptional undernutrition modifies endocrine profiles and hepatic gene expression in sheep.
abstract: This study investigated whether a 22-day period of undernutrition (half maintenance) could affect maternal endocrine responses and liver gene expression during early pregnancy (day 7). Thirty-five ewes were fed 1.5 (n = 15) or 0.5 (n = 20) their maintenance requirements and slaughtered on day 7 of the oestrus cycle or pregnancy (oestrus = day 0). Insulin, IGF, leptin and non-esterified fatty acids (NEFA) were determined on days -14, 0 and 7. Transcripts of the IGF family and adipokines receptors were determined in the liver by real-time RT-PCR. Underfed animals presented lower body weight and body condition, greater plasma concentration of NEFA, and lower plasma concentrations of leptin, insulin and IGF1 compared to adequately fed animals. Underfed ewes presented greater hepatic expression of IGFBP2 than well-fed ewes, but tended to have lesser expression of IGFBP5. While no effect of undernutrition on IGFBP4 and ADIPOR2 mRNA expressions was observed, they were increased by pregnancy in underfed animals. This study shows that undernutrition modifies endocrine profiles and hepatic gene expression of IGFBP2 and 5. The pregnancy status increased hepatic gene expression of IGFBP4 and ADIPOR2 mRNA in undernourished ewes.
authors.affiliation: Laboratorio de Técnicas Nucleares, Facultad de Veterinaria, Montevideo, Uruguay.
authors.forename: V
authors.initials: V
authors.lastname: de Brun
authors.affiliation: Laboratorio de Técnicas Nucleares, Facultad de Veterinaria, Montevideo, Uruguay.
authors.forename: A
authors.initials: A
authors.lastname: Meikle
authors.affiliation: Departamento de Producción Animal y Pasturas, Facultad de Agronomía, Montevideo, Uruguay.
authors.forename: A
authors.initials: A
authors.lastname: Casal
authors.affiliation: Laboratorio de Técnicas Nucleares, Facultad de Veterinaria, Montevideo, Uruguay.
authors.forename: M
authors.initials: M
authors.lastname: Sequeira
authors.affiliation: Departamento de Producción Animal y Ciencia de los Alimentos, Universidad de Zaragoza, Zaragoza, Spain.
authors.forename: I
authors.initials: I
authors.lastname: Contreras-Solís
authors.affiliation: Departamento de Producción Animal y Pasturas, Facultad de Agronomía, Montevideo, Uruguay.
authors.forename: M
authors.initials: M
authors.lastname: Carriquiry
authors.affiliation: Departamento de Producción Animal y Ciencia de los Alimentos, Universidad de Zaragoza, Zaragoza, Spain.
authors.forename: F
authors.initials: F
authors.lastname: Forcada
authors.affiliation: Departamento de Producción Animal y Ciencia de los Alimentos, Universidad de Zaragoza, Zaragoza, Spain.
authors.forename: C
authors.initials: C
authors.lastname: Sosa
authors.affiliation: Departamento de Producción Animal y Ciencia de los Alimentos, Universidad de Zaragoza, Zaragoza, Spain.
authors.forename: J A
authors.initials: JA
authors.lastname: Abecia
mesh_terms: D000821:Animal Feed; D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D004032:Diet / Q000662:veterinary; D030762:Estrous Cycle; D005230:Fatty Acids, Nonesterified / Q000097:blood; D005260:Female; D005508:Food Deprivation; D005786:Gene Expression Regulation / Q000502:physiology*; D007328:Insulin / Q000097:blood; D007334:Insulin-Like Growth Factor I / Q000378:metabolism; D020738:Leptin / Q000097:blood; D008099:Liver / Q000378:metabolism*; D008969:Molecular Sequence Data; D011247:Pregnancy; D012333:RNA, Messenger; D012756:Sheep / Q000097:blood / Q000378:metabolism*
chemical_list: D005230:Fatty Acids, Nonesterified; D007328:Insulin; D020738:Leptin; D012333:RNA, Messenger; D007334:Insulin-Like Growth Factor I
keywords: endocrine profiles; gene expression; liver; nutrition; sheep