title: Actinotalea suaedae sp. nov., isolated from the halophyte Suaeda physophora in Xinjiang, Northwest China.
abstract: A Gram-stain-positive, aerobic, non-motile, coryneform bacterium, designated strain EGI 60002(T), was isolated from the halophyte Suaeda physophora. Cells were coryneform shaped and polymorphic. Phylogenetic analysis based on 16S rRNA gene sequences showed that the new isolate was closely related to Actinotalea ferrariae CF5-4(T) (95.8 % gene sequence similarity). The peptidoglycan type of strain EGI 60002(T) was A4β, containing L-Orn-D-Ser-D-Asp. The cell-wall sugars were mannose, ribose, rhamnose and glucose. The major fatty acids (>5 %) of strain EGI 60002(T) were iso-C14:0, iso-C15:0, anteiso-C15:1 A and anteiso-C15:0. The predominant respiratory quinone was MK-10(H4). The major polar lipids were diphosphatidylglycerol (DPG), one unidentified phosphoglycolipid (PGL) and one unidentified phospholipid (PL1). The genomic DNA G+C content was 72.3 mol%. On the basis of morphological, physiological, chemotaxonomic data, and phylogenetic analysis, strain EGI 60002(T) should be classified as a novel species within the genus Actinotalea, for which the name Actinotalea suaedae sp. nov. is proposed. The type strain is EGI 60002T (=JCM 19624(T) = KACC 17839(T) = KCTC 29256(T)).
authors.affiliation: Key Laboratory of Biogeography and Bioresource in Arid Land, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Ürümqi, 830011, People's Republic of China.
authors.forename: Shuai
authors.initials: S
authors.lastname: Zhao
authors.forename: Li
authors.initials: L
authors.lastname: Li
authors.forename: Shan-Hui
authors.initials: SH
authors.lastname: Li
authors.forename: Hong-Fei
authors.initials: HF
authors.lastname: Wang
authors.forename: Wael N
authors.initials: WN
authors.lastname: Hozzein
authors.forename: Yong-Guang
authors.initials: YG
authors.lastname: Zhang
authors.forename: Mohammed A M
authors.initials: MA
authors.lastname: Wadaan
authors.forename: Wen-Jun
authors.initials: WJ
authors.lastname: Li
authors.forename: Chang-Yan
authors.initials: CY
authors.lastname: Tian
mesh_terms: D039903:Actinobacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D000332:Aerobiosis; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002241:Carbohydrates / Q000032:analysis; D002473:Cell Wall / Q000737:chemistry; D019663:Chenopodiaceae / Q000382:microbiology*; D002681:China; D016000:Cluster Analysis; D003600:Cytosol / Q000737:chemistry; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D008969:Molecular Sequence Data; D010457:Peptidoglycan / Q000032:analysis; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D011809:Quinones / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D055051:Salt-Tolerant Plants / Q000382:microbiology; D017422:Sequence Analysis, DNA
chemical_list: D002241:Carbohydrates; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D010457:Peptidoglycan; D010743:Phospholipids; D011809:Quinones; D012336:RNA, Ribosomal, 16S