title: Effects of American ginseng on pharmacokinetics of 5-fluorouracil in rats.
abstract: The pharmacokinetics of 5-fluorouracil (5-FU) in combination with or without American ginseng (seven-consecutive days oral dose) in rats were evaluated using liquid chromatography-electrospray ionization-mass spectrometry (LC-MS). Chromatographic separation was performed on a reverse LC column within a total run time of 6.5 min, which allowed for a relatively quick analysis. The limit of quantification for 5-FU was 15 ng/mL and this method was linear over 15-50,000 ng/mL. This method supported stabilizing determination of the plasma concentration of 5-FU over a period of 24 h. Precision both interday and intraday (coefficient of variation) was within 14% and accuracy (relative error) ranged from -5 to 14%. In view of the observed pharmacokinetic parameters, including maximum concentration, time to maximum concentration, area under the concentration-time curve (AUC), mean residence time, elimination half-life and clearance, our results showed no significant differences in all of the pharmacokinetic parameters between the ginseng co-treated group and 5-FU alone group. Some increase in AUC was observed in 5-FU plus ginseng group; however, the difference did not reach statistical significance compared with 5-FU alone. It appeared that American ginseng administration did not significantly alter the kinetics of 5-FU. More studies are still needed to confirm our results.
authors.affiliation: State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing, 210009, China.
authors.forename: Yi-Sheng
authors.initials: YS
authors.lastname: He
authors.forename: Wei
authors.initials: W
authors.lastname: Sun
authors.forename: Chong-Zhi
authors.initials: CZ
authors.lastname: Wang
authors.forename: Lian-Wen
authors.initials: LW
authors.lastname: Qi
authors.forename: Jie
authors.initials: J
authors.lastname: Yang
authors.forename: Ping
authors.initials: P
authors.lastname: Li
authors.forename: Xiao-Dong
authors.initials: XD
authors.lastname: Wen
authors.forename: Chun-Su
authors.initials: CS
authors.lastname: Yuan
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000097:blood / Q000493:pharmacokinetics*; D002851:Chromatography, High Pressure Liquid; D004347:Drug Interactions; D005472:Fluorouracil / Q000097:blood / Q000493:pharmacokinetics*; D006801:Humans; D008297:Male; D009369:Neoplasms / Q000097:blood / Q000188:drug therapy*; D005894:Panax / Q000737:chemistry*; D010936:Plant Extracts / Q000008:administration & dosage* / Q000097:blood; D051381:Rats; D053719:Tandem Mass Spectrometry
chemical_list: D000970:Antineoplastic Agents; D010936:Plant Extracts; D005472:Fluorouracil
keywords: 5-fluorouracil; American ginseng; LC-MS; herb-drug interaction; pharmacokinetics