title: Quantitative proteomic analysis of thylakoid from two microalgae (Haematococcus pluvialis and Dunaliella salina) reveals two different high light-responsive strategies.
abstract: Under high light (HL) stress, astaxanthin-accumulating Haematococcus pluvialis and β-carotene-accumulating Dunaliella salina showed different responsive patterns. To elucidate cellular-regulating strategies photosynthetically and metabolically, thylakoid membrane proteins in H. pluvialis and D. salina were extracted and relatively quantified after 0 h, 24 h and 48 h of HL stress. Proteomic analysis showed that three subunits of the cytochrome b6/f complex were greatly reduced under HL stress in H. pluvialis, while they were increased in D. salina. Additionally, the major subunits of both photosystem (PS) II and PSI reaction center proteins were first reduced and subsequently recovered in H. pluvialis, while they were gradually reduced in D. salina. D. salina also showed a greater ability to function using the xanthophyll-cycle and the cyclic photosynthetic electron transfer pathway compared to H. pluvialis. We propose a reoriented and effective HL-responsive strategy in H. pluvialis, enabling it to acclimate under HL. The promising metabolic pathway described here contains a reorganized pentose phosphate pathway, Calvin cycle and glycolysis pathway participating in carbon sink formation under HL in H. pluvialis. Additionally, the efficient carbon reorientation strategy in H. pluvialis was verified by elevated extracellular carbon assimilation and rapid conversion into astaxanthin.
authors.affiliation: Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, 7 Nanhai Road, 266071 Qingdao, China.
authors.forename: Wenhui
authors.initials: W
authors.lastname: Gu
authors.affiliation: 1] Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, 7 Nanhai Road, 266071 Qingdao, China [2] University of Chinese Academy of Sciences, 100049 Beijing, China.
authors.forename: Huan
authors.initials: H
authors.lastname: Li
authors.affiliation: 1] Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, 7 Nanhai Road, 266071 Qingdao, China [2] University of Chinese Academy of Sciences, 100049 Beijing, China.
authors.forename: Peipei
authors.initials: P
authors.lastname: Zhao
authors.affiliation: College of Marine Science and Engineering, Tianjin University of Science and Technology, 300457 Tianjin, China.
authors.forename: Ruixue
authors.initials: R
authors.lastname: Yu
authors.affiliation: College of Marine Science and Engineering, Tianjin University of Science and Technology, 300457 Tianjin, China.
authors.forename: Guanghua
authors.initials: G
authors.lastname: Pan
authors.affiliation: Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, 7 Nanhai Road, 266071 Qingdao, China.
authors.forename: Shan
authors.initials: S
authors.lastname: Gao
authors.affiliation: Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, 7 Nanhai Road, 266071 Qingdao, China.
authors.forename: Xiujun
authors.initials: X
authors.lastname: Xie
authors.affiliation: Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, 7 Nanhai Road, 266071 Qingdao, China.
authors.forename: Aiyou
authors.initials: A
authors.lastname: Huang
authors.affiliation: Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, 7 Nanhai Road, 266071 Qingdao, China.
authors.forename: Linwen
authors.initials: L
authors.lastname: He
authors.affiliation: Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, 7 Nanhai Road, 266071 Qingdao, China.
authors.forename: Guangce
authors.initials: G
authors.lastname: Wang
mesh_terms: D002244:Carbon / Q000378:metabolism; D000460:Chlorophyta; D008027:Light; D053858:Metabolic Networks and Pathways / Q000235:genetics; D058086:Microalgae / Q000235:genetics / Q000502:physiology; D010788:Photosynthesis / Q000235:genetics*; D045332:Photosystem II Protein Complex / Q000235:genetics*; D040901:Proteomics; D020524:Thylakoids / Q000235:genetics / Q000378:metabolism*; D024341:Xanthophylls / Q000235:genetics / Q000378:metabolism
chemical_list: D045332:Photosystem II Protein Complex; D024341:Xanthophylls; D002244:Carbon; C005948:astaxanthine