title: High-fat diet and chronic stress reduce central pressor and tachycardic effects of apelin in Sprague-Dawley rats.
abstract: Central application of apelin elevates blood pressure and influences neuroendocrine responses to stress and food consumption. However, it is not known whether the central cardiovascular effects of apelin depend also on caloric intake or chronic stress. The purpose of the present study was to determine the effects of intracerebroventricular administration of apelin on blood pressure (mean arterial blood pressure) and heart rate in conscious Sprague-Dawley rats consuming either a normal-fat diet (NFD) or high-fat diet (HFD) for 12 weeks. During the last 4 weeks of the food regime, the rats were exposed (NFDS and HFDS groups) or not exposed (NFDNS and HFDNS groups) to chronic stress. Each group was divided into two subgroups receiving intracerebroventricular infusions of either vehicle or apelin. Apelin elicited significant increase of mean arterial blood pressure and heart rate in the NFDNS rats. This effect was abolished in the HFDNS, HFDS and NFDS groups. HFD resulted in a significant elevation of blood concentrations of total cholesterol, triglycerides glucose and insulin. Chronic stress reduced plasma concentration of total and high-density lipoprotein cholesterol, and increased plasma corticosterone concentration and APJ receptor mRNA expression in the hypothalamus, whereas a combination of a HFD with chronic stress resulted in the elevation of plasma triglycerides, total cholesterol and low-density lipoprotein cholesterol, and in increased plasma corticosterone concentration, apelin concentration and APJ receptor mRNA expression in the hypothalamus. It is concluded that a HFD and chronic stress result in significant suppression of the central pressor action of apelin, and cause significant though not unidirectional changes of metabolic and endocrine parameters.
authors.affiliation: Department of Experimental and Clinical Physiology, Laboratory of Centre for Preclinical Research, Medical University of Warsaw, Warsaw, Poland.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Cudnoch-Jedrzejewska
authors.forename: Ryszard
authors.initials: R
authors.lastname: Gomolka
authors.forename: Ewa
authors.initials: E
authors.lastname: Szczepanska-Sadowska
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Czarzasta
authors.forename: Robert
authors.initials: R
authors.lastname: Wrzesien
authors.forename: Lukasz
authors.initials: L
authors.lastname: Koperski
authors.forename: Liana
authors.initials: L
authors.lastname: Puchalska
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Wsol
mesh_terms: D000818:Animals; D000073861:Apelin; D015415:Biomarkers / Q000097:blood; D001794:Blood Pressure / Q000502:physiology*; D002908:Chronic Disease; D059305:Diet, High-Fat / Q000639:trends; D036341:Intercellular Signaling Peptides and Proteins / Q000097:blood*; D008297:Male; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D013315:Stress, Psychological / Q000097:blood* / Q000523:psychology; D013610:Tachycardia / Q000097:blood* / Q000517:prevention & control / Q000523:psychology
chemical_list: D000073861:Apelin; C498579:Apln protein, rat; D015415:Biomarkers; D036341:Intercellular Signaling Peptides and Proteins
keywords: apelin; blood pressure; obesity; stress