title: Molecular characterization of blaNDM-5 carried on an IncFII plasmid in an Escherichia coli isolate from a nontraveler patient in Spain.
abstract: A carbapenem-resistant Escherichia coli isolate (sequence type 448 [ST448]) was recovered from a urine culture of a female patient with no recent record of traveling. PCR screening identified the presence of bla(NDM-5), bla(TEM-1), bla(OXA-1), bla(CMY-42), and rmtB. bla(NDM-5) was carried in a conjugative IncFII-type plasmid (90 kb) together with bla(TEM-1) and rmtB. The genetic environment of bla(NDM-5) showed a structure similar to those of pMC-NDM and pGUE-NDM, identified in Poland and France in E. coli of African and Indian origin, respectively.
authors.affiliation: Department of Clinical Microbiology, School of Medicine, CRESIB-Hospital Clinic, University of Barcelona, Barcelona, Spain.
authors.forename: Cristina
authors.initials: C
authors.lastname: Pitart
authors.affiliation: Department of Clinical Microbiology, School of Medicine, CRESIB-Hospital Clinic, University of Barcelona, Barcelona, Spain.
authors.forename: Mar
authors.initials: M
authors.lastname: Solé
authors.affiliation: Department of Clinical Microbiology, School of Medicine, CRESIB-Hospital Clinic, University of Barcelona, Barcelona, Spain ignasi.roca@cresib.cat jvila@ub.edu.
authors.forename: Ignasi
authors.initials: I
authors.lastname: Roca
authors.affiliation: Department of Clinical Microbiology, School of Medicine, CRESIB-Hospital Clinic, University of Barcelona, Barcelona, Spain.
authors.forename: Angely
authors.initials: A
authors.lastname: Román
authors.affiliation: Department of Infectious Diseases, School of Medicine, Hospital Clinic, University of Barcelona, Barcelona, Spain.
authors.forename: Asunción
authors.initials: A
authors.lastname: Moreno
authors.affiliation: Department of Clinical Microbiology, School of Medicine, CRESIB-Hospital Clinic, University of Barcelona, Barcelona, Spain ignasi.roca@cresib.cat jvila@ub.edu.
authors.forename: Jordi
authors.initials: J
authors.lastname: Vila
authors.affiliation: Department of Clinical Microbiology, School of Medicine, CRESIB-Hospital Clinic, University of Barcelona, Barcelona, Spain.
authors.forename: Francesc
authors.initials: F
authors.lastname: Marco
mesh_terms: D000368:Aged; D001426:Bacterial Proteins / Q000235:genetics; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics*; D004927:Escherichia coli Infections / Q000188:drug therapy / Q000382:microbiology*; D029968:Escherichia coli Proteins / Q000235:genetics; D005260:Female; D005798:Genes, Bacterial / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics; D013030:Spain / Q000453:epidemiology; D018440:beta-Lactam Resistance / Q000235:genetics*; D001618:beta-Lactamases / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D001618:beta-Lactamases; C063912:carbapenemase