title: First identification of proteins involved in motility of Mycoplasma gallisepticum.
abstract: Mycoplasma gallisepticum, the most pathogenic mycoplasma in poultry, is able to glide over solid surfaces. Although this gliding motility was first observed in 1968, no specific protein has yet been shown to be involved in gliding. We examined M. gallisepticum strains and clonal variants for motility and found that the cytadherence proteins GapA and CrmA were required for gliding. Loss of GapA or CrmA resulted in the loss of motility and hemadsorption and led to drastic changes in the characteristic flask-shape of the cells. To identify further genes involved in motility, a transposon mutant library of M. gallisepticum was generated and screened for motility-deficient mutants, using a screening assay based on colony morphology. Motility-deficient mutants had transposon insertions in gapA and the neighbouring downstream gene crmA. In addition, insertions were seen in gene mgc2, immediately upstream of gapA, in two motility-deficient mutants. In contrast to the GapA/CrmA mutants, the mgc2 motility mutants still possessed the ability to hemadsorb. Complementation of these mutants with a mgc2-hexahistidine fusion gene restored the motile phenotype. This is the first report assigning specific M. gallisepticum proteins to involvement in gliding motility.
authors.affiliation: Department of Pathobiology, Institute of Bacteriology, Mycology and Hygiene, University of Veterinary Medicine Vienna, Veterinaerplatz 1, A-1210, Vienna, Austria. ivana.indikova@vetmeduni.ac.at.
authors.forename: Ivana
authors.initials: I
authors.lastname: Indikova
authors.affiliation: Department of Pathobiology, Institute of Bacteriology, Mycology and Hygiene, University of Veterinary Medicine Vienna, Veterinaerplatz 1, A-1210, Vienna, Austria. martin_vronka2@yahoo.de.
authors.forename: Martin
authors.initials: M
authors.lastname: Vronka
authors.affiliation: Department of Pathobiology, Institute of Bacteriology, Mycology and Hygiene, University of Veterinary Medicine Vienna, Veterinaerplatz 1, A-1210, Vienna, Austria. michael.szostak@vetmeduni.ac.at.
authors.forename: Michael P
authors.initials: MP
authors.lastname: Szostak
mesh_terms: D018829:Adhesins, Bacterial / Q000235:genetics* / Q000378:metabolism; D009154:Mutation; D045686:Mycoplasma gallisepticum / Q000235:genetics / Q000502:physiology*
chemical_list: D018829:Adhesins, Bacterial