title: Investigation of the effect of nocodazole on the PI3K/Akt signaling pathway in COS7 cells.
abstract: A number of studies have demonstrated that nocodazole suppresses Akt phosphorylation; however, the underlying molecular mechanism remains unclear. In the current study, the mechanism of nocodazole‑induced suppression of Akt phosphorylation was investigated. The cell cycle was analyzed using flow cytometry and EGF receptor dimerization was evaluated through a cross-linking assay. Immunoprecipitation experiments were performed to investigate the interaction between P85 and EGFR and cell migration was evaluated through a wound healing assay. COS7 cells were observed to be rounded following a 24‑h treatment with nocodazole, and the results revealed that ~45% of COS7 cells were arrested at the G2/M phase and that the cyclin B1 expression level was greatly increased. EGF‑mediated Akt phosphorylation was markedly inhibited in nocodazole‑treated cells. In addition, the levels of internalized EGF‑EGFR complexes in nocodazole‑treated cells were reduced, and EGF‑EGFR dimerization was found to be affected by nocodazole. Akt phosphorylation in COS7 cells was demonstrated to be overridden by AG1478 and wortmannin. The results also showed that p85 did not bind to activated EGFR in nocodazole‑treated cells, and that nocodazole and protein inhibitors reduced cell migration. In summary, these results indicate that nocodazole inhibits the PI3K/Akt pathway by interfering with the binding of p85 binding to activated EGFR and further affects the growth of cells.
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, P.R. China.
authors.forename: Huaiping
authors.initials: H
authors.lastname: Shi
authors.affiliation: Department of Hepatobiliary Surgery, Shaanxi Provincial People's Hospital, Xi'an City, Shaanxi 710068, P.R. China.
authors.forename: Wujun
authors.initials: W
authors.lastname: Wu
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, P.R. China.
authors.forename: Jun
authors.initials: J
authors.lastname: Luo
mesh_terms: D000818:Animals; D019556:COS Cells; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002465:Cell Movement / Q000187:drug effects; D002522:Chlorocebus aethiops; D056744:Cyclin B1 / Q000378:metabolism; D066246:ErbB Receptors / Q000378:metabolism; D016195:G2 Phase / Q000187:drug effects; D057809:HEK293 Cells; D006801:Humans; D015739:Nocodazole / Q000494:pharmacology*; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism*; D010766:Phosphorylation / Q000187:drug effects; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects*
chemical_list: D056744:Cyclin B1; D066246:ErbB Receptors; D015739:Nocodazole; D019869:Phosphatidylinositol 3-Kinases; D051057:Proto-Oncogene Proteins c-akt