title: [Genome sequencing and the molecular evolutionary analysis of a SFTSV isolated from Zhejiang province].
abstract: OBJECTIVE
  To sequence the whole genome and to analyze the molecular and evolutionary of Severe fever with thrombocytopenia syndrome bunyavirus (SFTSV) isolated from Zhejiang province.
  
  
  METHODS
  Viral RNA was extracted from the specimens and detected by quantitative real-time RT-PCR. SFTSV strain was isolated. A total of 17 overlapping fragments covering the whole genome were amplified by RT-PCR. And the entire genomes were formed by sequencing and assembly the fragments. The SFTSV sequence of Zhejiang strain was compared with the sequences of SFTSV that have been published to generate the phylogenetic tree. And the SFTSV sequence of Zhejiang strain was compared with the sequences of strains of the genus phlebovirus in the Bunyaviridae family and analysis of homology.
  
  
  RESULTS
  SFTSV strain was isolated from SFTSV infection positive serum successfully. The genomic fragments were amplified by RT-PCR. A total of 3 cDNA sections were formed by sequencing and assembly the fragments. The S segment contained 1 745 nucleotides. The M fragment contained 3 378 nucleotides, and the L segment contained 6 368 nucleotides. Molecular phylogenetic analysis result showed SFTSV Zhejiang strain had the highest similarity with Japan/SPL004A/2013 strain. The similarity of the S segment was 98%. The similarity of the M fragment was 97%. And it was 98% that of the L fragment. Meanwhile, the comparison results also confirmed the Zhejiang strain belonged to the genus phlebovirus.
  
  
  CONCLUSIONS
  SFTSV Zhejiang strain of isolated from SFTSV infection positive serum successfully. And the genome sequencing was complete molecular evolution analysis shows SFTSV Zhejiang strain has the maximum similarity with SFTSV Japan strain.
authors.affiliation: College of Chemistry and Life Sciences, Zhejiang Normal University, Jinhua 321004, China.
authors.forename: Cen
authors.initials: C
authors.lastname: Feng
authors.forename: Lei
authors.initials: L
authors.lastname: Zhang
authors.forename: Yi
authors.initials: Y
authors.lastname: Sun
authors.forename: Bin
authors.initials: B
authors.lastname: Shao
authors.forename: Haiyan
authors.initials: H
authors.lastname: Mao
authors.forename: Jianmin
authors.initials: J
authors.lastname: Jiang
authors.affiliation: Email: zhangyjtt@126.com.
authors.forename: Yanjun
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D001483:Base Sequence; D002043:Bunyaviridae; D002044:Bunyaviridae Infections; D019143:Evolution, Molecular; D016678:Genome; D016856:Phlebovirus; D010802:Phylogeny; D012367:RNA, Viral; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D012367:RNA, Viral