title: Long noncoding RNA GAS5 affects cell proliferation and predicts a poor prognosis in patients with colorectal cancer.
abstract: Colorectal cancer (CRC) is the third most common type of cancer worldwide. Recent studies have shown that lncRNAs play important roles in carcinogenesis. The aim of this study was to explore the role of lncRNA GAS5 in CRC. Real-time PCR was performed to investigate the expression of GAS5 in tumor tissues and corresponding non-tumor colorectal tissues from 66 patients with CRC. The lower expression of GAS5 was significantly correlated with large tumor size, low histological grade and advanced TNM stage. Multivariate analyses revealed that GAS5 expression served as an independent predictor for overall survival (P = 0.034). Further experiments revealed that overexpressed GAS5 significantly repressed the proliferation both in vitro and in vivo. In conclusion, our results suggest that GAS5, as a growth regulator, may serve as a candidate prognostic biomarker in human colorectal cancer.
authors.affiliation: Cancer Research and Therapy Center, The Second Affiliated Hospital of Southeast University, Nanjing, Jiangsu, China.
authors.forename: Dandan
authors.initials: D
authors.lastname: Yin
authors.forename: Xuezhi
authors.initials: X
authors.lastname: He
authors.forename: Erbao
authors.initials: E
authors.lastname: Zhang
authors.forename: Rong
authors.initials: R
authors.lastname: Kong
authors.forename: Wei
authors.initials: W
authors.lastname: De
authors.forename: Zhihong
authors.initials: Z
authors.lastname: Zhang
mesh_terms: D000368:Aged; D000818:Animals; D049109:Cell Proliferation / Q000502:physiology*; D015179:Colorectal Neoplasms / Q000175:diagnosis* / Q000378:metabolism*; D005260:Female; D045325:HCT116 Cells; D006801:Humans; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D008875:Middle Aged; D009367:Neoplasm Staging; D011237:Predictive Value of Tests; D011379:Prognosis; D062085:RNA, Long Noncoding / Q000378:metabolism*; D023041:Xenograft Model Antitumor Assays / Q000379:methods
chemical_list: C561567:GAS5 long non-coding RNA, human; D062085:RNA, Long Noncoding