title: Anti-obesity effects of hispidin and Alpinia zerumbet bioactives in 3T3-L1 adipocytes.
abstract: Obesity and its related disorders have become leading metabolic diseases. In the present study, we used 3T3-L1 adipocytes to investigate the anti-obesity activity of hispidin and two related compounds that were isolated from Alpinia zerumbet (alpinia) rhizomes. The results showed that hispidin, dihydro-5,6-dehydrokawain (DDK), and 5,6-dehydrokawain (DK) have promising anti-obesity properties. In particular, all three compounds significantly increased intracellular cyclic adenosine monophosphate (cAMP) concentrations by 81.2% ± 0.06%, 67.0% ± 1.62%, and 56.9% ± 0.19%, respectively. Hispidin also stimulated glycerol release by 276.4% ± 0.8% and inhibited lipid accumulation by 47.8% ± 0.16%. Hispidin and DDK decreased intracellular triglyceride content by 79.5% ± 1.37% and 70.2% ± 1.4%, respectively, and all three compounds inhibited glycerol-3-phosphate dehydrogenase (GPDH) and pancreatic lipase, with hispidin and DDK being the most potent inhibitors. Finally, none of the three compounds reduced 3T3-L1 adipocyte viability. These results highlight the potential for developing hispidin and its derivatives as anti-obesity compounds.
authors.affiliation: Department of Bioscience and Biotechnology, The United Graduate School of Agricultural Sciences, Kagoshima University, Korimoto 1-21-24, Kagoshima 890-8580, Japan. ptbetu@yahoo.com.
authors.forename: Pham Thi Be
authors.initials: PT
authors.lastname: Tu
authors.affiliation: Department of Bioscience and Biotechnology, Faculty of Agriculture, University of the Ryukyus, Senbaru 1, Nishihara-cho, Okinawa 903-0129, Japan. b986097@agr.u-ryukyu.ac.jp.
authors.forename: Shinkichi
authors.initials: S
authors.lastname: Tawata
mesh_terms: D041721:3T3-L1 Cells; D017667:Adipocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D050156:Adipogenesis / Q000187:drug effects; D032429:Alpinia / Q000737:chemistry*; D000818:Animals; D019440:Anti-Obesity Agents / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects; D000242:Cyclic AMP / Q000378:metabolism; D005990:Glycerol / Q000378:metabolism; D005993:Glycerolphosphate Dehydrogenase / Q000037:antagonists & inhibitors / Q000378:metabolism; D008049:Lipase / Q000037:antagonists & inhibitors / Q000378:metabolism; D008055:Lipids / Q000032:analysis; D051379:Mice; D009765:Obesity / Q000188:drug therapy*; D010936:Plant Extracts / Q000494:pharmacology*; D011753:Pyrones / Q000494:pharmacology*; D014280:Triglycerides / Q000378:metabolism
chemical_list: D019440:Anti-Obesity Agents; D008055:Lipids; D010936:Plant Extracts; D011753:Pyrones; D014280:Triglycerides; C052297:5,6-dehydrokawain; C052296:7,8-dihydro-5,6-dehydrokawain; D000242:Cyclic AMP; D005993:Glycerolphosphate Dehydrogenase; D008049:Lipase; D005990:Glycerol; C007946:hispidin