title: Fractionation analysis of manganese in Turkish hazelnuts (Corylus avellana L.) by inductively coupled plasma-mass spectrometry.
abstract: In this study, an analytical fractionation scheme based on water, diethyl ether, n-hexane, and methanol extractions has been developed to identify manganese-bound fractions. Additionally, in vitro simulated gastric and intestinal digestion, n-octanol extraction, and activated carbon adsorption were used to interpret the manganese-bound structures in hazelnuts in terms of bioaccessibility. The total content of manganese in the samples was determined by inductively coupled plasma-mass spectrometry after microwave-assisted digestion, and additional validation was performed using atomic absorption spectroscopy. Water fractions were further evaluated by high-performance liquid chromatography hyphenated to inductively coupled plasma-mass spectrometry for the identification of water-soluble manganese fractions in hazelnut samples. The limits of detection and quantification were 3.6 and 12.0 μg L(-1), respectively, based on peak height.
authors.affiliation: Department of Chemistry, Faculty of Arts and Sciences, University of Uludag , Gorukle Campus, 16059 Nilufer-Bursa, Turkey.
authors.forename: Umran Seven
authors.initials: US
authors.lastname: Erdemir
authors.forename: Seref
authors.initials: S
authors.lastname: Gucer
mesh_terms: D000327:Adsorption; D031211:Corylus / Q000737:chemistry* / Q000378:metabolism; D004063:Digestion; D006801:Humans; D008345:Manganese / Q000032:analysis* / Q000302:isolation & purification* / Q000378:metabolism; D013058:Mass Spectrometry; D008954:Models, Biological; D010936:Plant Extracts / Q000032:analysis* / Q000302:isolation & purification* / Q000378:metabolism; D012639:Seeds / Q000737:chemistry* / Q000378:metabolism; D013270:Stomach
chemical_list: D010936:Plant Extracts; D008345:Manganese
keywords: ICP-MS; bioaccessibility; fractionation; hazelnut (Corylus avellana L.); manganese