title: Biocomposite nanofibrous strategies for the controlled release of biomolecules for skin tissue regeneration.
abstract: Nanotechnology and tissue engineering have enabled engineering of nanostructured strategies to meet the current challenges in skin tissue regeneration. Electrospinning technology creates porous nanofibrous scaffolds to mimic extracellular matrix of the native tissues. The present study was performed to gain some insights into the applications of poly(l-lactic acid)-co-poly-(ε-caprolactone) (PLACL)/silk fibroin (SF)/vitamin E (VE)/curcumin (Cur) nanofibrous scaffolds and to assess their potential for being used as substrates for the culture of human dermal fibroblasts for skin tissue engineering. PLACL/SF/VE/Cur nanofibrous scaffolds were fabricated by electrospinning and characterized by fiber morphology, membrane porosity, wettability, mechanical strength, and chemical properties by Fourier transform infrared (FTIR) analysis. Human dermal fibroblasts were cultured on these scaffolds, and the cell scaffold interactions were analyzed by cell proliferation, cell morphology, secretion of collagen, expression of F-actin, and 5-chloromethylfluorescein diacetate (CMFDA) dye. The electrospun nanofiber diameter was obtained between 198±4 nm and 332±13 nm for PLACL, PLACL/SF, PLACL/SF/VE, and PLACL/SF/VE/Cur nanofibrous scaffolds. FTIR analysis showed the presence of the amide groups I, II, and III, and a porosity of up to 92% obtained on these nanofibrous scaffolds. The results showed that the fibroblast proliferation, cell morphology, F-actin, CMFDA dye expression, and secretion of collagen were significantly increased in PLACL/SF/VE/Cur when compared to PLACL nanofibrous scaffolds. The accessibility of human dermal fibroblasts cultured on PLACL/SF/VE/Cur nanofibrous scaffolds proved to be a potential scaffold for skin tissue regeneration.
authors.affiliation: Cellular and Molecular Epigenetics Laboratory, Lee Kong Chian School of Medicine, Nanyang Technological University, Singapore.
authors.forename: Chinnasamy
authors.initials: C
authors.lastname: Gandhimathi
authors.affiliation: Center for Nanofibers and Nanotechnology, Nanoscience and Nanotechnology Initiative, Faculty of Engineering, National University of Singapore, Singapore.
authors.forename: Jayarama Reddy
authors.initials: JR
authors.lastname: Venugopal
authors.affiliation: Center for Nanofibers and Nanotechnology, Nanoscience and Nanotechnology Initiative, Faculty of Engineering, National University of Singapore, Singapore.
authors.forename: Velmurugan
authors.initials: V
authors.lastname: Bhaarathy
authors.affiliation: Center for Nanofibers and Nanotechnology, Nanoscience and Nanotechnology Initiative, Faculty of Engineering, National University of Singapore, Singapore.
authors.forename: Seeram
authors.initials: S
authors.lastname: Ramakrishna
authors.affiliation: Cellular and Molecular Epigenetics Laboratory, Lee Kong Chian School of Medicine, Nanyang Technological University, Singapore.
authors.forename: Srinivasan Dinesh
authors.initials: SD
authors.lastname: Kumar
mesh_terms: D002448:Cell Adhesion; D048430:Cell Shape; D002478:Cells, Cultured; D003094:Collagen; D003474:Curcumin / Q000737:chemistry / Q000493:pharmacokinetics; D003692:Delayed-Action Preparations; D005347:Fibroblasts; D005349:Fibroins / Q000737:chemistry; D005452:Fluoresceins; D006801:Humans; D057139:Nanofibers / Q000737:chemistry*; D011091:Polyesters / Q000737:chemistry; D012038:Regeneration; D012867:Skin / Q000166:cytology* / Q000378:metabolism; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds
chemical_list: D003692:Delayed-Action Preparations; D005452:Fluoresceins; D011091:Polyesters; C550605:poly(lactic acid-co-epsilon-caprolactone); C069306:5-chloromethylfluorescein; D003094:Collagen; D005349:Fibroins; D003474:Curcumin
keywords: Sirius red staining; curcumin release; fibroblast; poly(l-lactic acid)-co-poly-(ε-caprolactone); silk fibroin; skin tissue regeneration