title: Plenoptic projection fluorescence tomography.
abstract: A new method to obtain the three-dimensional localization of fluorochrome distributions in micrometric samples is presented. It uses a microlens array coupled to the image port of a standard microscope to obtain tomographic data by a filtered back-projection algorithm. Scanning of the microlens array is proposed to obtain a dense data set for reconstruction. Simulation and experimental results are shown and the implications of this approach in fast 3D imaging are discussed.
authors.forename: Ignacio
authors.initials: I
authors.lastname: Iglesias
authors.forename: Jorge
authors.initials: J
authors.lastname: Ripoll
mesh_terms: D000465:Algorithms; D005453:Fluorescence; D021621:Imaging, Three-Dimensional / Q000379:methods*; D019047:Phantoms, Imaging; D015203:Reproducibility of Results; D014054:Tomography / Q000295:instrumentation*