title: Two-allergen model reveals complex relationship between IgE crosslinking and degranulation.
abstract: Allergy is an immune response to complex mixtures of multiple allergens, yet current models use a single synthetic allergen. Multiple allergens were modeled using two well-defined tetravalent allergens, each specific for a distinct IgE, thus enabling a systematic approach to evaluate the effect of each allergen and percentage of allergen-specific IgE on mast cell degranulation. We found the overall degranulation response caused by two allergens is additive for low allergen concentrations or low percent specific IgE, does not change for moderate allergen concentrations with moderate to high percent specific IgE, and is reduced for high allergen concentrations with moderate to high percent specific IgE. These results provide further evidence that supraoptimal IgE crosslinking decreases the degranulation response and establishes the two-allergen model as a relevant experimental system to elucidate mast cell degranulation mechanisms.
authors.affiliation: Department of Chemical and Biomolecular Engineering, University of Notre Dame, Notre Dame, IN 46556, USA.
authors.forename: Michael W
authors.initials: MW
authors.lastname: Handlogten
authors.affiliation: Department of Chemical and Biomolecular Engineering, University of Notre Dame, Notre Dame, IN 46556, USA.
authors.forename: Peter E
authors.initials: PE
authors.lastname: Deak
authors.affiliation: Department of Chemical and Biomolecular Engineering, University of Notre Dame, Notre Dame, IN 46556, USA; Department of Chemistry and Biochemistry, University of Notre Dame, Notre Dame, IN 46556, USA; Advanced Diagnostics and Therapeutics, University of Notre Dame, Notre Dame, IN 46556, USA. Electronic address: bbilgicer@nd.edu.
authors.forename: Basar
authors.initials: B
authors.lastname: Bilgicer
mesh_terms: D019297:2,4-Dinitrophenol / Q000737:chemistry; D000485:Allergens / Q000737:chemistry / Q000276:immunology*; D000818:Animals; D000937:Antigen-Antibody Reactions; D002417:Cattle; D015550:Cell Degranulation; D003619:Dansyl Compounds / Q000737:chemistry; D007073:Immunoglobulin E / Q000276:immunology / Q000378:metabolism*; D008407:Mast Cells / Q000166:cytology / Q000502:physiology*; D008954:Models, Biological; D051381:Rats; D012710:Serum Albumin, Bovine / Q000737:chemistry / Q000276:immunology
chemical_list: D000485:Allergens; D003619:Dansyl Compounds; D012710:Serum Albumin, Bovine; D007073:Immunoglobulin E; D019297:2,4-Dinitrophenol