title: MONOPTEROS directly activates the auxin-inducible promoter of the Dof5.8 transcription factor gene in Arabidopsis thaliana leaf provascular cells.
abstract: MONOPTEROS (MP) is an auxin-responsive transcription factor that is required for primary root formation and vascular development, whereas Dof5.8 is a Dof-class transcription factor whose gene is expressed in embryos as well as the pre- and procambial cells in the leaf primordium in Arabidopsis thaliana. In this study, it is shown that MP directly activates the Dof5.8 promoter. Although no apparent phenotype of the single dof5.8 mutants was found, phenotypic analysis with the mp dof5.8 double mutants revealed that mutations within Dof5.8 enhanced the phenotype of a weak allele of mp, with an increase in the penetrance of the 'rootless' phenotype and a reduction in the number of cotyledons. Furthermore, interestingly, although mp mutants showed reduced vascular pattern complexity in cotyledons, the mp dof5.8 double mutants displayed both more simplex and more complex vascular patterns in individual cotyledons. These results imply that the product of Dof5.8 whose expression is regulated by MP at least in part might be involved in multiple processes controlled by MP.
authors.affiliation: Biotechnology Research Center, The University of Tokyo, Yayoi 1-1-1, Bunkyo-ku, Tokyo 113-8657, Japan.
authors.forename: Mineko
authors.initials: M
authors.lastname: Konishi
authors.affiliation: Department of Biological Sciences, University of Alberta, CW-405 Biological Sciences Building, Edmonton, Alberta, Canada T6G 2E9.
authors.forename: Tyler J
authors.initials: TJ
authors.lastname: Donner
authors.affiliation: Department of Biological Sciences, University of Alberta, CW-405 Biological Sciences Building, Edmonton, Alberta, Canada T6G 2E9.
authors.forename: Enrico
authors.initials: E
authors.lastname: Scarpella
authors.affiliation: Biotechnology Research Center, The University of Tokyo, Yayoi 1-1-1, Bunkyo-ku, Tokyo 113-8657, Japan asyanagi@mail.ecc.u-tokyo.ac.jp.
authors.forename: Shuichi
authors.initials: S
authors.lastname: Yanagisawa
mesh_terms: D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant; D007210:Indoleacetic Acids / Q000378:metabolism; D018515:Plant Leaves / Q000235:genetics* / Q000378:metabolism; D011401:Promoter Regions, Genetic / Q000235:genetics; D012097:Repressor Proteins / Q000235:genetics* / Q000378:metabolism*; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism*
chemical_list: C000602370:ATDOF5.8 protein, Arabidopsis; D029681:Arabidopsis Proteins; D004268:DNA-Binding Proteins; C000606088:Dof5.8 protein, Arabidopsis; D007210:Indoleacetic Acids; C111371:MONOPTEROS protein, Arabidopsis; D012097:Repressor Proteins; D014157:Transcription Factors
keywords: Arabidopsis thaliana; Dof transcription factor; MONOPTEROS; auxin response; embryo development; vascular development.