title: Crenobacter luteus gen. nov., sp. nov., isolated from a hot spring.
abstract: A slightly thermophilic, Gram-staining-negative and strictly aerobic bacteria, designated strain YIM 78141(T), was isolated from a sediment sample collected at Hehua hot spring, Tengchong, Yunnan province, south-west China. Cells of the strain were short-rod-shaped and colonies were yellowish and circular. The strain grew at pH 6.0-10.0 (optimum, pH 8.0-9.0) and 10-55 °C (optimum, 40-50 °C). Phylogenetic analyses based on 16S rRNA gene sequence comparison demonstrated that strain YIM 78141(T) belongs to the family Neisseriaceae, and strain YIM 78141(T) also showed low levels of 16S rRNA gene sequence similarity (below 93.4%) with all other genera in this family. The only quinone was ubiquinone 8 and the genomic DNA G+C content was 67.3 mol%. Major fatty acids (>5%) were C12:0, C16:0, C18:1ω7c and summed feature 3. The polar lipids consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylmethylethanolamine, phospholipids of unknown structure containing aminoglycophospholipid and three unidentified polar lipids. On the basis of the morphological, physiological and biochemical characteristics as well as genotypic data, this strain should be classified as a representative of a novel genus and species of the family Neisseriaceae, for which the name Crenobacter luteus gen. nov., sp. nov. is proposed. The type strain is YIM 78141(T) ( =BCRC 80650(T) =KCTC 32558(T) =DSM 27258(T)).
authors.affiliation: Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education, Yunnan Institute of Microbiology, Yunnan University, Kunming 650091, PR China.
authors.forename: Lei
authors.initials: L
authors.lastname: Dong
authors.affiliation: Department of Life Sciences and Technology, Xinxiang Medical University, Xinxiang 453003, PR China.|Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education, Yunnan Institute of Microbiology, Yunnan University, Kunming 650091, PR China.
authors.forename: Hong
authors.initials: H
authors.lastname: Ming
authors.affiliation: Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education, Yunnan Institute of Microbiology, Yunnan University, Kunming 650091, PR China.
authors.forename: En-Min
authors.initials: EM
authors.lastname: Zhou
authors.affiliation: Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education, Yunnan Institute of Microbiology, Yunnan University, Kunming 650091, PR China.
authors.forename: Yi-Rui
authors.initials: YR
authors.lastname: Yin
authors.affiliation: Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education, Yunnan Institute of Microbiology, Yunnan University, Kunming 650091, PR China.
authors.forename: Lan
authors.initials: L
authors.lastname: Liu
authors.affiliation: Department of Life Sciences and Technology, Xinxiang Medical University, Xinxiang 453003, PR China.
authors.forename: Hui-Geng
authors.initials: HG
authors.lastname: Feng
authors.affiliation: Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education, Yunnan Institute of Microbiology, Yunnan University, Kunming 650091, PR China.
authors.forename: Wen-Dong
authors.initials: WD
authors.lastname: Xian
authors.affiliation: College of Fisheries, Henan Normal University, Xinxiang 453007, PR China.
authors.forename: Guo-Xing
authors.initials: GX
authors.lastname: Nie
authors.affiliation: State Key Laboratory of Biocontrol, Key Laboratory of Biodiversity Dynamics and Conservation of Guangdong Higher Education Institutes, College of Ecology and Evolution, Sun Yat-Sen University, Guangzhou 510275, PR China.|Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education, Yunnan Institute of Microbiology, Yunnan University, Kunming 650091, PR China.
authors.forename: Wen-Jun
authors.initials: WJ
authors.lastname: Li
mesh_terms: D001420:Bacteria, Aerobic / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D019015:Geologic Sediments / Q000382:microbiology; D016946:Gram-Negative Aerobic Rods and Cocci / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D045482:Hot Springs / Q000382:microbiology*; D008969:Molecular Sequence Data; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D014451:Ubiquinone / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D014451:Ubiquinone; C030778:ubiquinone 8