title: Babesia infection in naturally exposed pet dogs from a north-eastern state (Assam) of India: detection by microscopy and polymerase chain reaction.
abstract: The objective of the study was to detect Babesia infections in pet dogs of a north-eastern state of India. The diagnostic efficacy of Babesia infection by polymerase chain reaction (PCR) technique has been compared with microscopy examination. For this, a total of 111 blood samples of pet dogs presented at clinical complex of the College of Veterinary Science, Guwahati, Assam with clinical signs suspected for Babesia infection subjected to the study. A total of 44 (39.63 %) dogs were diagnosed as positive for Babesia infections after microscopic examination. Among these, Babesia canis infection was diagnosed in 5 dogs (4.50 %) and B. gibsoni infection in 39 (35.13 %) dogs microscopically in Giemsa stained blood smears. Molecular diagnosis using PCR detected 63 (56.75 %) dogs positive for Babesia infection. Single infection with B. canis was found in 9 (8.10 %) dogs while B. gibsoni alone was detected in 3 (2.70 %) dogs. Mixed infections by both these species were detected in 51 (45.94 %) dogs. Overall, PCR detected 54 (48.64 %) dogs as B. gibsoni and 60 (54.05 %) dogs as B. canis positive.
authors.affiliation: Division of Animal Health, ICAR Research Complex for NEH Region, Umroi Road, Umiam, 793103 Meghalaya India.
authors.forename: R
authors.initials: R
authors.lastname: Laha
authors.affiliation: Department of Parasitology, College of Veterinary Science, Khanapara, Guwahati, Assam 781022 India.
authors.forename: K
authors.initials: K
authors.lastname: Bhattacharjee
authors.affiliation: Department of Parasitology, College of Veterinary Science, Khanapara, Guwahati, Assam 781022 India.
authors.forename: P C
authors.initials: PC
authors.lastname: Sarmah
authors.affiliation: Division of Animal Health, ICAR Research Complex for NEH Region, Umroi Road, Umiam, 793103 Meghalaya India.
authors.forename: M
authors.initials: M
authors.lastname: Das
authors.affiliation: Division of Animal Health, ICAR Research Complex for NEH Region, Umroi Road, Umiam, 793103 Meghalaya India.
authors.forename: A
authors.initials: A
authors.lastname: Goswami
authors.affiliation: Division of Animal Health, ICAR Research Complex for NEH Region, Umroi Road, Umiam, 793103 Meghalaya India.
authors.forename: D
authors.initials: D
authors.lastname: Sarma
authors.affiliation: Division of Animal Health, ICAR Research Complex for NEH Region, Umroi Road, Umiam, 793103 Meghalaya India.
authors.forename: A
authors.initials: A
authors.lastname: Sen
keywords: Babesiacanis; Babesiagibsoni; Detection; Dog; India; Microscopy; PCR