title: Activation of metabotropic glutamate receptors regulates ribosomes of cochlear nucleus neurons.
abstract: The brain stem auditory system of the chick is an advantageous model for examining changes that occur as a result of deafness. Elimination of acoustic input through cochlear ablation results in the eventual death of approximately 30% of neurons in the chick cochlear nucleus, nucleus magnocellularis (NM). One early change following deafness is an alteration in NM ribosomes, evidenced both by a decrease in protein synthesis and reduction in antigenicity for Y10B, a monoclonal antibody that recognizes a ribosomal epitope. Previous studies have shown that mGluR activation is necessary to maintain Y10B antigenicity and NM viability. What is still unclear, however, is whether or not mGluR activation is sufficient to prevent deafness-induced changes in these neurons, or if other activity-dependent factors are also necessary. The current study investigated the ability of mGluR activation to regulate cochlear nucleus ribosomes in the absence of auditory nerve input. In vitro methods were employed to periodically pressure eject glutamate or mGluR agonists over neurons on one side of a slice preparation leaving the opposite side of the same slice untreated. Immunohistochemistry was then performed using Y10B in order to assess ribosomal changes. Application of glutamate and both group I and II selective mGluR agonists effectively rescued ribosomal antigenicity on the treated side of the slice in comparison to ribosomes on the untreated side. These findings suggest that administration of mGluR agonists is sufficient to reduce the early interruption of normal ribosomal integrity that is typically seen following loss of auditory nerve activity.
authors.affiliation: Program in Neuroscience, Department of Psychology, Florida State University, Tallahassee, FL, United States of America.
authors.forename: Kathryn L
authors.initials: KL
authors.lastname: Carzoli
authors.affiliation: Program in Neuroscience, Department of Psychology, Florida State University, Tallahassee, FL, United States of America.
authors.forename: Richard L
authors.initials: RL
authors.lastname: Hyson
mesh_terms: D000818:Animals; D001933:Brain Stem / Q000378:metabolism* / Q000473:pathology; D001643:Bridged Bicyclo Compounds / Q000494:pharmacology; D002645:Chickens; D003056:Cochlear Nerve / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D017626:Cochlear Nucleus / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D003638:Deafness / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D018604:Epitope Mapping; D018698:Glutamic Acid / Q000494:pharmacology; D006801:Humans; D008734:Methoxyhydroxyphenylglycol / Q000031:analogs & derivatives / Q000494:pharmacology; D014176:Protein Biosynthesis / Q000187:drug effects; D018094:Receptors, Metabotropic Glutamate / Q000819:agonists / Q000096:biosynthesis* / Q000378:metabolism; D012270:Ribosomes / Q000187:drug effects / Q000378:metabolism*
chemical_list: D001643:Bridged Bicyclo Compounds; D018094:Receptors, Metabotropic Glutamate; D018698:Glutamic Acid; D008734:Methoxyhydroxyphenylglycol; C104753:eglumetad; C010117:3,4-dihydroxyphenylglycol