title: A key role of microRNA-29b in suppression of osteosarcoma cell proliferation and migration via modulation of VEGF.
abstract: MicroRNA (miRNA) is a small, non-coding RNAs and it could post-transcriptionally related gene expression by negatively regulating the stability or translational efficiency of their target genes. Previous studies have reported the antineoplastic effect of microRNA-29b (miR-29b) in several kinds of cancers. The aim of this study was to investigate the potential role of miR-29b in human osteosarcoma pathogenesis. Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) was performed to determine the expression level of miR-29b in 20 osteosarcoma specimens and adjacent normal bone tissues. The proliferation, apoptosis, migration and invasion were employed to detect the effect of miR-29b on the osteosarcoma cell line MG63. The results showed that miR-29b expression was relatively decreased in osteosarcoma specimens compared with adjacent normal tissues. Overexpression of miR-29b suppressed MG63 cell proliferation, migration and invasion. Meanwhile miR-29b could induce apoptosis of MG63. Besides, miR-29b directly targets VEGF and over-expression of miR-29b led to down-regulation of VEGF protein level, In conclusions, miR-29b may play an important role in osteosarcoma progression, which might negatively regulate the expression of VEGF and suppresses proliferation and induces apoptosis of MG63 cell line.
authors.affiliation: Department of Orthopedics, Third Xiangya Hospital, Central South University Changsha 410013, Hunan Province, China.
authors.forename: Kexiang
authors.initials: K
authors.lastname: Zhang
authors.affiliation: Department of Orthopedics, Third Xiangya Hospital, Central South University Changsha 410013, Hunan Province, China.
authors.forename: Chaoyue
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Department of Orthopedics, Third Xiangya Hospital, Central South University Changsha 410013, Hunan Province, China.
authors.forename: Li
authors.initials: L
authors.lastname: Liu
authors.affiliation: Department of Orthopedics, Third Xiangya Hospital, Central South University Changsha 410013, Hunan Province, China.
authors.forename: Jiahui
authors.initials: J
authors.lastname: Zhou
mesh_terms: D017209:Apoptosis; D001859:Bone Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D009361:Neoplasm Invasiveness; D012516:Osteosarcoma / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D015398:Signal Transduction; D013997:Time Factors; D014162:Transfection; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism*
chemical_list: C532045:MIRN29a microRNA, human; D035683:MicroRNAs; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A
keywords: miR-29b; micro RNA; osteosarcoma; vascular endothelial growth factor