title: Rapid synthesis of biocompatible silver nanoparticles using aqueous extract of Rosa damascena petals and evaluation of their anticancer activity.
abstract: OBJECTIVE
  To optimize the process parameters involved in the green synthesis of silver nanoparticles (G-SNPs) by aqueous extract of Rosa damascena petals and to evaluate the biocompatibility and anti cancer activity of the synthesized silver nanoparticles against human lung adenocarcinoma (A549).
  
  
  METHODS
  The process variables that include concentration of extract, mixing ratio of reactants, silver salt concentration and interaction time were analyzed. The compatibility of the G-SNPs was verified by incubating with erythrocytes and the anticancer property of the G-SNPs against A549 cells was performed by MTT assay.
  
  
  RESULTS
  Formation of G-SNPs was confirmed by the visual change in the colour of the reaction mixture from pale yellow to brown yellow. Surface plasmon resonance of synthesized G-SNPs was observed at 420 nm; the size of G-SNPs were analyzed by DLS and found to be in the range of (84.00±10.08) nm. Field emission scanning electron microscope and high resolution transmission electron microscopy analysis confirmed that the G-SNPs were fairly spherical. Fourier transform infrared spectroscopy spectroscopy and X-ray diffraction revealed the characteristic peaks of G-SNPs. Energy dispersive X-ray analysis showed a signal of silver around 3 keV. The synthesized G-SNPs exhibited anticancer activity as evidenced by the MTT assay. IC50 value of G-SNPs was found to be 80 μg/mL.
  
  
  CONCLUSIONS
  The results of the present study suggest that G-SNPs can be synthesized rapidly within first minute of the reaction; they are biocompatible and possess anticancer activity against human lung adenocarcinoma.
authors.affiliation: Department of Biochemistry, University of Madras, Guindy Campus, Chennai-600 025, India.
authors.forename: Balaji
authors.initials: B
authors.lastname: Venkatesan
authors.affiliation: Department of Biochemistry, University of Madras, Guindy Campus, Chennai-600 025, India.
authors.forename: Vimala
authors.initials: V
authors.lastname: Subramanian
authors.affiliation: Department of Biochemistry, University of Madras, Guindy Campus, Chennai-600 025, India.
authors.forename: Anusha
authors.initials: A
authors.lastname: Tumala
authors.affiliation: Department of Biochemistry, University of Madras, Guindy Campus, Chennai-600 025, India. Electronic address: vellaie@gmail.com.
authors.forename: Elangovan
authors.initials: E
authors.lastname: Vellaichamy
keywords: Anticancer activity; Green synthesis; Hemolytic activity assay; Human lung cancer cell line; Rosa damascena; Silver nanoparticles