title: Biofunctionalized silver nanoparticles as a novel colorimetric probe for melamine detection in raw milk.
abstract: Nanoparticles have emerged as a promising analytical tool for monitoring food adulteration and safety. In the present study, silver nanoparticles (AgNPs) were synthesized using leaves' extract of Jatropha gossypifolia. AgNPs revealed a characteristic surface plasmon resonance (SPR) peak at 419 nm and have spherical and grain shape with size range between 18 and 30 nm. A selective and rapid method of melamine detection in raw milk was developed with the use of these biofunctionalized AgNPs. The color change, deviation in SPR spectra, and change in the absorption ratio (A500 /A419 ) of AgNPs occurred after an AgNPs-melamine interaction. The detection limit for melamine up to 2 μM (252 ppb) was attained with this method, which is quite lower than safety level recommendations of regulatory bodies demonstrating sensitivity of the method. Dynamicx light scattering and transmission electron microscopy analyses exhibited an increase in hydrodynamic diameter and size of AgNPs after melamine interaction. Melamine sensing by AgNPs was investigated by different physicochemical and thermal analyses.
authors.affiliation: School of Life Sciences, North Maharashtra University, Jalgaon, India.
authors.forename: Hemant P
authors.initials: HP
authors.lastname: Borase
authors.affiliation: School of Life Sciences, North Maharashtra University, Jalgaon, India.|JDMVPS Nutan Maratha College, Jalgaon, India.
authors.forename: Chandrashekhar D
authors.initials: CD
authors.lastname: Patil
authors.affiliation: School of Life Sciences, North Maharashtra University, Jalgaon, India.
authors.forename: Rahul B
authors.initials: RB
authors.lastname: Salunkhe
authors.affiliation: School of Life Sciences, North Maharashtra University, Jalgaon, India.
authors.forename: Rahul K
authors.initials: RK
authors.lastname: Suryawanshi
authors.affiliation: School of Life Sciences, North Maharashtra University, Jalgaon, India.
authors.forename: Bipinchandra K
authors.initials: BK
authors.lastname: Salunke
authors.affiliation: School of Life Sciences, North Maharashtra University, Jalgaon, India.|North Maharashtra Microbial Culture Collection Centre (NMCC), North Maharashtra University, Jalgaon, India.
authors.forename: Satish V
authors.initials: SV
authors.lastname: Patil
mesh_terms: D000818:Animals; D003116:Color; D003124:Colorimetry / Q000379:methods*; D005504:Food Analysis; D031286:Jatropha / Q000737:chemistry; D057230:Limit of Detection; D053768:Metal Nanoparticles / Q000737:chemistry*; D008892:Milk / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry; D018515:Plant Leaves / Q000737:chemistry; D012834:Silver / Q000737:chemistry*; D020349:Surface Plasmon Resonance / Q000379:methods*; D013696:Temperature; D013997:Time Factors; D014227:Triazines / Q000032:analysis* / Q000737:chemistry
chemical_list: D010936:Plant Extracts; D014227:Triazines; D012834:Silver; C011907:melamine
keywords: colorimetric probe; melamine sensor; nanoparticle aggregation; silver nanoparticles; zeta potential