title: Secretory leukocyte protease inhibitor is a proliferation and survival factor for pancreatic cancer cells.
abstract: OBJECTIVE
  A variety of inflammatory cytokines have been demonstrated to participate in tumorigenesis and progression. Secretory leukocyte protease inhibitor (SLPI) has been demonstrated to show a broad-spectrum of anti-inflammatory effects. This study investigates the expression of SLPI in human pancreatic cancer tissues and cells as well as its biological effects in human pancreatic cancer cells.
  
  
  METHODS
  Reverse transcription-polymerase chain reaction, immunohistochemistry, and Western blot were used to detect SLPI mRNA and protein levels in human pancreatic cancer tissues, adjacent tissues, and pancreatic cancer Bxpc-3 and Panc-1 cells. Knockout of SLPI expression was established by recombinant viral vector expressing short hairpin RNA (shRNA) targeting SLPI. Cell viability was analyzed by MTT assay. Cell apoptosis was detected by Hochest33258 staining and flow cytometry assay.
  
  
  RESULTS
  Higher SLPI expression was observed in pancreatic tissues, Bxpc-3 cells, and Panc-1 cells compared to the peritumoral tissues (p < 0.01). SLPI expression in Bxpc-3 and Panc-1 cells was effectively silenced by shRNA (p < 0.001). Silencing of SLPI expression significantly reduced cell viability, inhibited cell proliferation, and induced cell apoptosis (p < 0.001).
  
  
  CONCLUSIONS
  Abnormal over-expression of SLPI in pancreatic cancer cells may be associated with the development of disease through its roles in promoting cancer cell survival and proliferation as well as anti-apoptosis. SLPI can be used as a target for developing targeted therapy of pancreatic cancer.
authors.affiliation: Department of Stomatology, Xiangya Hospital, Central South University, Changsha, 410008, China.
authors.forename: J
authors.initials: J
authors.lastname: Zuo
authors.forename: C
authors.initials: C
authors.lastname: Zhang
authors.forename: C
authors.initials: C
authors.lastname: Ren
authors.forename: D
authors.initials: D
authors.lastname: Pang
authors.forename: Y
authors.initials: Y
authors.lastname: Li
authors.forename: X
authors.initials: X
authors.lastname: Xie
authors.forename: Z
authors.initials: Z
authors.lastname: Tang
authors.forename: X
authors.initials: X
authors.lastname: Jiang
mesh_terms: D000328:Adult; D000368:Aged; D017209:Apoptosis; D015153:Blotting, Western / Q000379:methods; D049109:Cell Proliferation; D002470:Cell Survival; D005260:Female; D005434:Flow Cytometry; D020868:Gene Silencing; D005822:Genetic Vectors; D006801:Humans; D008297:Male; D008875:Middle Aged; D010190:Pancreatic Neoplasms / Q000235:genetics* / Q000473:pathology*; D012333:RNA, Messenger / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D053481:Secretory Leukocyte Peptidase Inhibitor / Q000235:genetics*; D014162:Transfection; D014407:Tumor Cells, Cultured; D055815:Young Adult
chemical_list: D012333:RNA, Messenger; C506814:SLPI protein, human; D053481:Secretory Leukocyte Peptidase Inhibitor