title: Asymmetric giant lipid vesicle fabrication.
abstract: Synthetic lipid bilayers have long been used as models of cell membranes. The compositional asymmetry in the eukaryotic plasma membrane is a key chemical characteristic of this membrane that has traditionally been difficult to reproduce in synthetic systems. In this chapter, we describe recent technologies for fabricating compositionally asymmetric giant unilamellar lipid vesicles (GUVs) and provide detailed protocols for a microfluidic-based fabrication technique.
authors.affiliation: Mork Family Department of Chemical Engineering and Materials Science, University of Southern California, 925 Bloom Walk, HED 216, Los Angeles, CA, 90089-1211, USA.
authors.forename: Peichi C
authors.initials: PC
authors.lastname: Hu
authors.forename: Noah
authors.initials: N
authors.lastname: Malmstadt
mesh_terms: D001710:Biotin / Q000737:chemistry; D004867:Equipment Design; D008051:Lipid Bilayers / Q000737:chemistry; D008055:Lipids / Q000737:chemistry; D008081:Liposomes; D044085:Microfluidics / Q000295:instrumentation / Q000379:methods*; D008967:Molecular Biology / Q000379:methods*; D010713:Phosphatidylcholines / Q000737:chemistry; D053835:Unilamellar Liposomes
chemical_list: D008051:Lipid Bilayers; D008055:Lipids; D008081:Liposomes; D010713:Phosphatidylcholines; D053835:Unilamellar Liposomes; D001710:Biotin; C017251:1,2-oleoylphosphatidylcholine