title: Dentinogenic responses after direct pulp capping of miniature swine teeth with Biodentine.
abstract: BACKGROUND
  The aim of this study was to evaluate pulpal responses after experimental direct pulp capping of mechanically exposed teeth with a new calcium silicate-based dentin replacement material.
  
  
  METHODS
  Thirty-four anterior and posterior teeth of 3 miniature swine were used. Class V or I cavities were prepared on the buccal or occlusal surfaces, respectively. Pulpal exposures were further performed using a round carbide bur 0.8 mm in diameter. Exposures were treated with white MTA Angelus (Angelus, Londrina, PR, Brazil) or Biodentine (Septodont, Saint Maur des Fosses, France), and the cavities were further restored with Biodentine. The pulpal tissue responses were histologically assessed at postoperative periods of 3 and 8 weeks. Data were statistically analyzed using the Kruskal Wallis and the Mann-Whitney U tests.
  
  
  RESULTS
  Inflammatory infiltration or pulp tissue necrosis was not found in any of the specimens. All teeth showed mineralized matrix formation in the form of a complete hard tissue bridge composed of osteodentin or osteodentin followed by a discontinuous or continuous reparative dentin zone. A significantly higher thickness of the hard tissue bridge was found in the group of teeth treated with Biodentine at both 3 and 8 weeks. A number of teeth, which were under root development at the onset of the experimental procedures, exhibited ectopic pulp calcification.
  
  
  CONCLUSIONS
  The application of both calcium silicate-based materials in direct contact with the mechanically exposed pulp of healthy miniature swine teeth led to pulp repair with complete hard tissue bridge formation. The thickness of hard tissue bridges was significantly higher after pulp capping with Biodentine.
authors.affiliation: Department of Operative Dentistry, School of Dentistry, Aristotle University of Thessaloniki, Thessaloniki, Greece.
authors.forename: Christina
authors.initials: C
authors.lastname: Tziafa
authors.affiliation: Department of Operative Dentistry, School of Dentistry, Aristotle University of Thessaloniki, Thessaloniki, Greece.
authors.forename: Eugenia
authors.initials: E
authors.lastname: Koliniotou-Koumpia
authors.affiliation: Department of Clinical Sciences, Veterinary School, Aristotle University of Thessaloniki, Thessaloniki, Greece.
authors.forename: Serafim
authors.initials: S
authors.lastname: Papadimitriou
authors.affiliation: Department of Endodontology, School of Dentistry, Aristotle University of Thessaloniki, Thessaloniki, Greece. Electronic address: dtziaf@dent.auth.gr.
authors.forename: Dimitrios
authors.initials: D
authors.lastname: Tziafas
mesh_terms: D000818:Animals; D001729:Bismuth / Q000627:therapeutic use; D017610:Calcium Compounds / Q000627:therapeutic use*; D003738:Dental Cements / Q000627:therapeutic use; D003782:Dental Pulp / Q000187:drug effects / Q000473:pathology; D003784:Dental Pulp Calcification / Q000209:etiology; D003785:Dental Pulp Capping / Q000379:methods*; D003789:Dental Pulp Exposure / Q000188:drug therapy; D003790:Dental Pulp Necrosis / Q000517:prevention & control; D003809:Dentin, Secondary / Q000187:drug effects* / Q000473:pathology; D003810:Dentinogenesis / Q000187:drug effects*; D008963:Molar / Q000187:drug effects; D009805:Odontogenesis / Q000187:drug effects; D010087:Oxides / Q000627:therapeutic use; D057872:Pulp Capping and Pulpectomy Agents / Q000627:therapeutic use*; D011671:Pulpitis / Q000517:prevention & control; D011897:Random Allocation; D017640:Silicates / Q000627:therapeutic use*; D013552:Swine; D013556:Swine, Miniature; D013997:Time Factors
chemical_list: D017610:Calcium Compounds; D003738:Dental Cements; C475480:MTA-Angelus; D010087:Oxides; D057872:Pulp Capping and Pulpectomy Agents; D017640:Silicates; C506393:tricalcium silicate; D001729:Bismuth
keywords: Biodentine; calcium silicate; direct pulp capping; mineral trioxide aggregate; miniature swine teeth; pulp capping materials; reparative dentin