title: Carnoy's solution is an adequate tissue fixative for routine surgical pathology, preserving cell morphology and molecular integrity.
abstract: OBJECTIVE
  To compare Carnoy's solution (CS) and 10% neutral buffered formalin solution (NBF) as tissue fixatives in colorectal cancer specimens.
  
  
  RESULTS
  Surgical specimens from patients with colorectal cancer were analysed. Three groups were studied, as follows: group 1 consisted of 16 paired samples fixed in CS and NBF; and groups 2 and 3 consisted of 14 prospective and 80 retrospective samples, respectively, both randomized for fixation in CS or NBF. Groups 1 and 2 were analysed for amount, quality and integrity of DNA. Morphological analysis, including some of the usual special stains and polymerase chain reaction (PCR), were also performed for group 1, and Sanger sequencing for group 2. Immunohistochemical (IHC) reactions for mismatch repair proteins were studied in groups 1 and 3. Fixative performances were similar for morphology, special stains, and IHC reactions, as well as for the amount, quality and integrity of extracted DNA. PCR amplification was not possible in two cases from CS group 1. Sanger sequencing gave conclusive results for the CS samples tested.
  
  
  CONCLUSIONS
  Carnoy's solution and NBF are equivalent fixatives for colorectal cancer specimens and are adequate for routine utilization in surgical and molecular pathology.
authors.affiliation: Cancer Institute(ICESP), University of Sao Paulo School of Medicine, Sao Paulo, Brazil.
authors.forename: Marina A
authors.initials: MA
authors.lastname: Pereira
authors.forename: Andre R
authors.initials: AR
authors.lastname: Dias
authors.forename: Sheila F
authors.initials: SF
authors.lastname: Faraj
authors.forename: Cinthya dos S
authors.initials: Cdos S
authors.lastname: Cirqueira
authors.forename: Michele T
authors.initials: MT
authors.lastname: Tomitao
authors.forename: Sergio Carlos
authors.initials: SC
authors.lastname: Nahas
authors.forename: Ulysses
authors.initials: U
authors.lastname: Ribeiro
authors.forename: Evandro S
authors.initials: ES
authors.lastname: de Mello
mesh_terms: D019342:Acetic Acid; D000230:Adenocarcinoma / Q000175:diagnosis; D002725:Chloroform; D015179:Colorectal Neoplasms / Q000175:diagnosis; D004247:DNA / Q000032:analysis / Q000302:isolation & purification; D000431:Ethanol; D006801:Humans; D007150:Immunohistochemistry; D057089:Pathology, Molecular / Q000379:methods*; D010340:Pathology, Surgical / Q000379:methods*; D016133:Polymerase Chain Reaction; D016707:Tissue Fixation / Q000379:methods*
chemical_list: C033474:Carnoy's solution; D000431:Ethanol; D002725:Chloroform; D004247:DNA; D019342:Acetic Acid
keywords: Carnoy's solution; DNA extraction; formalin; immunohistochemistry; tissue fixation