title: Colivelin ameliorates amyloid β peptide-induced impairments in spatial memory, synaptic plasticity, and calcium homeostasis in rats.
abstract: Amyloid β peptide (Aβ) has been thought to be neurotoxic and responsible for the impairment of learning and memory in Alzheimer's disease (AD). Humanin (HN), a 24 amino acid polypeptide first identified from the unaffected occipital lobe of an AD patient, is believed to be neuroprotective against the AD-related neurotoxicity. In this study, we investigated the neuroprotective effects of Colivelin (CLN), a novel HN derivative, against Aβ by using behavioral test, in vivo electrophysiological recording, and intracellular calcium imaging. Our results showed that intrahippocampal injection of CLN (0.2 nmol) effectively prevented Aβ25-35 (4 nmol)-induced deficits in spatial learning and memory of rats in Morris water maze test; the suppression of in vivo hippocampal long term potentiation (LTP) by Aβ25-35 was nearly completely prevented by CLN; in addition, CLN pretreatment also effectively inhibited Aβ25-35-induced calcium overload in primary cultured hippocampal neurons. These results indicate that CLN has significant neuroprotective properties against Aβ, and CLN may holds great promise for the treatment and prevention of AD.
authors.affiliation: Department of Physiology, Key Laboratory of Cellular Physiology, Ministry of Education, Shanxi Medical University, Taiyuan, China.
authors.forename: Mei-Na
authors.initials: MN
authors.lastname: Wu
authors.forename: Li-Wei
authors.initials: LW
authors.lastname: Zhou
authors.forename: Zhao-Jun
authors.initials: ZJ
authors.lastname: Wang
authors.forename: Wei-Na
authors.initials: WN
authors.lastname: Han
authors.forename: Jun
authors.initials: J
authors.lastname: Zhang
authors.forename: Xiao-Jie
authors.initials: XJ
authors.lastname: Liu
authors.forename: Jia-Qing
authors.initials: JQ
authors.lastname: Tong
authors.forename: Jin-Shun
authors.initials: JS
authors.lastname: Qi
mesh_terms: D016229:Amyloid beta-Peptides / Q000633:toxicity; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002478:Cells, Cultured; D006624:Hippocampus / Q000166:cytology; D006706:Homeostasis / Q000187:drug effects*; D047908:Intracellular Signaling Peptides and Proteins / Q000627:therapeutic use*; D017774:Long-Term Potentiation / Q000187:drug effects; D018782:Maze Learning / Q000187:drug effects; D008569:Memory Disorders / Q000139:chemically induced / Q000188:drug therapy*; D009473:Neuronal Plasticity / Q000187:drug effects*; D009474:Neurons / Q000187:drug effects; D010446:Peptide Fragments / Q000633:toxicity; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D016229:Amyloid beta-Peptides; C508523:Colivelin; D047908:Intracellular Signaling Peptides and Proteins; D010446:Peptide Fragments; C074636:amyloid beta-protein (25-35); D002118:Calcium
keywords: Morris water maze; amyloid β-peptide (Aβ); colivelin (CLN); intracellular calcium overload; long term potentiation (LTP)