title: Promoted megakaryocytic differentiation of K562 cells through oxidative stress caused by near ultraviolet irradiation.
abstract: Reactive oxygen species (ROS) have been proven to be important activators for various cellular activities, including cell differentiation. Several reports showed the necessity of ROS during cell differentiation of the megakaryocytic (MK) lineage. In this study, we employed near ultraviolet (near-UV) irradiation to generate endogenous oxidative stress in an MK differentiation process of K562 cells with phorbol 12-myristate 13-acetate (PMA) induction. A significant increase in the intracellular ROS level was detected on day 1 after near-UV irradiation. In the initial stage of differentiation, a shifted fraction of G1 and G2 phase cells was obtained using near-UV irradiation, giving an increased percentage of G2 phase cells (up from 31.1 to 68.7%). The near-UV irradiation-induced upregulation of the p21 gene, which is a cell cycle inhibitor, suggested that the G2 phase cells were prevented from undergoing cell division. It was found that the percentage of high ploidy (8N and 16N) cells was enhanced significantly at the later stage of the K562 cell culture with near-UV irradiation. Moreover, time-lapse analysis showed that near-UV irradiation encouraged the expression of CD41, a specific surface marker of megakaryocytes. This is the first report that the elevated oxidative stress through the near-UV irradiation promoted the MK differentiation of PMA-induced K562 cells.
authors.affiliation: Division of Chemical Engineering, Department of Materials Engineering Science, Graduate School of Engineering Science, Osaka University, 1-3 Machikaneyama-cho, Toyonaka, Osaka, 560-8531, Japan.
authors.forename: Retno Wahyu
authors.initials: RW
authors.lastname: Nurhayati
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Ojima
authors.forename: Naoki
authors.initials: N
authors.lastname: Nomura
authors.forename: Masahito
authors.initials: M
authors.lastname: Taya
mesh_terms: D002453:Cell Cycle; D002454:Cell Differentiation / Q000528:radiation effects*; D006801:Humans; D020014:K562 Cells; D008533:Megakaryocytes / Q000502:physiology* / Q000528:radiation effects; D018384:Oxidative Stress; D017382:Reactive Oxygen Species / Q000378:metabolism; D014466:Ultraviolet Rays
chemical_list: D017382:Reactive Oxygen Species