title: Evaluation of association between common genetic variants on chromosome 9p21 and coronary artery disease in Turkish population.
abstract: OBJECTIVE
  Coronary artery disease (CAD), which develops from complex interactions between genetic and enviromental factors, is a leading cause of death worldwide. Based on genome-wide association studies (GWAS), the chromosomal region 9p21 has been identified as the most relevant locus presenting a strong association with CAD in different populations. The aim of the present study was to investigate the association of two SNPs on chromosome 9p21 on susceptibility to CAD and the effect of these SNPs along with cardiovascular risk factors on the severity of CAD in the Turkish population.
  
  
  METHODS
  This study had an observational case-control design. We genotyped 460 subjects, aged 30-65 years, to investigate the association of 2 SNPs (rs1333049, rs2383207) on chromosome 9p21 and CAD risk in Turkish population. Real-time polymerase chain reaction (RT-PCR) was used to analyze the 2 SNPs in CAD patients and healthy controls. The genotype and allelic variations of these SNPs with the severity of CAD was also assessed using semi-quantitative methods such as the Gensini score. Student's t test and multiple regression analysis were used for statistical analysis.
  
  
  RESULTS
  The SNPs rs1333049 and rs2383207 were found to be associated with CAD with an adjusted OR of 1.81 (95% Cl 1.05-3.12) and 2.12 (95% CI 1.19-4.10) respectively. After adjustment of CAD risk factors such as smoking, family history of CAD and diabetes, the homozygous AA genotype for rs2383207 increased the CAD risk with an OR 3.69. Also a very strong association was found between rs1333049 and rs2383207 and Gensini scores representing the severity of CAD (p<0.001).
  
  
  CONCLUSIONS
  The rs2383207 and rs1333049 SNPs on 9p21 chromosome were significantly associated with the risk and severity of CAD in the Turkish population.
authors.affiliation: Department of Cardiology, Cerrahpaşa Medical Faculty, İstanbul University; İstanbul-Turkey. altugcakmak@hotmail.com.
authors.forename: Hüseyin Altuğ
authors.initials: HA
authors.lastname: Çakmak
authors.forename: Burcu
authors.initials: B
authors.lastname: Bayoğlu
authors.forename: Eser
authors.initials: E
authors.lastname: Durmaz
authors.forename: Günay
authors.initials: G
authors.lastname: Can
authors.forename: Bilgehan
authors.initials: B
authors.lastname: Karadağ
authors.forename: Müjgan
authors.initials: M
authors.lastname: Cengiz
authors.forename: Vural Ali
authors.initials: VA
authors.lastname: Vural
authors.forename: Hüsniye
authors.initials: H
authors.lastname: Yüksel
mesh_terms: D000328:Adult; D000368:Aged; D016022:Case-Control Studies; D002899:Chromosomes, Human, Pair 9 / Q000235:genetics*; D003324:Coronary Artery Disease / Q000235:genetics* / Q000473:pathology; D005260:Female; D020022:Genetic Predisposition to Disease; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide; D012720:Severity of Illness Index; D014421:Turkey; D044465:White People / Q000235:genetics