title: The rabbit shunt model of subarachnoid haemorrhage.
abstract: Aneurysmal subarachnoid haemorrhage (SAH) is a disease with devastating complications that leads to stroke, permanent neurological deficits and death. Clinical and ex-perimental work has demonstrated the importance of the contribution of delayed cerebral vasospasm (DCVS) indepen-dent early events to mortality, morbidity and functional out-come after SAH. In order to elucidate processes involved in early brain injury (EBI), animal models that reflect acute events of aneurysmal bleeding, such as increase in intracranial pressure (ICP) and decrease in cerebral perfusion pressure, are needed. In the presented arterial shunt model, bleeding is initially driven by the pressure gradient between mean arterial blood pressure and ICP. SAH dynamics (flow rate, volume and duration) depend on physiological reactions and local anatomical intrathecal (cistern) conditions. During SAH, ICP reaches a plateau close to diastolic arterial blood pressure and the blood flow stops. Historical background, anaesthesia, perioperative care and monitoring, SAH induction, technical considerations and advantages and limitations of the rabbit blood shunt SAH model are discussed in detail. Awareness of technical details, physiological characteristics and appropriate monitoring methods guarantees successful implementation of the rabbit blood shunt model and allows the study of both EBI and DCVS after SAH.
authors.affiliation: Cerebrovascular Research Laboratory of the Department of Intensive Care Medicine, University Hospital and University of Bern, Bern, Switzerland, serge.marbacher@ksa.ch.
authors.forename: Serge
authors.initials: S
authors.lastname: Marbacher
authors.forename: Edin
authors.initials: E
authors.lastname: Nevzati
authors.forename: Davide
authors.initials: D
authors.lastname: Croci
authors.forename: Salome
authors.initials: S
authors.lastname: Erhardt
authors.forename: Carl
authors.initials: C
authors.lastname: Muroi
authors.forename: Stephan M
authors.initials: SM
authors.lastname: Jakob
authors.forename: Javier
authors.initials: J
authors.lastname: Fandino
mesh_terms: D000714:Anastomosis, Surgical; D000818:Animals; D001921:Brain / Q000503:physiopathology; D002946:Cisterna Magna / Q000601:surgery; D004195:Disease Models, Animal; D005260:Female; D007427:Intracranial Pressure; D008297:Male; D011817:Rabbits; D013345:Subarachnoid Hemorrhage / Q000209:etiology* / Q000601:surgery*; D020301:Vasospasm, Intracranial / Q000209:etiology*