title: Expression of gamma-aminobutyric acid type A receptor α2 subunit in the dorsal root ganglion of rats with sciatic nerve injury.
abstract: The γ-aminobutyric acid neurotransmitter in the spinal cord dorsal horn plays an important role in pain modulation through primary afferent-mediated presynaptic inhibition. The weakening of γ-aminobutyric acid-mediated presynaptic inhibition may be an important cause of neuropathic pain. γ-aminobutyric acid-mediated presynaptic inhibition is related to the current strength of γ-aminobutyric acid A receptor activation. In view of this, the whole-cell patch-clamp technique was used here to record the change in muscimol activated current of dorsal root ganglion neurons in a chronic constriction injury model. Results found that damage in rat dorsal root ganglion neurons following application of muscimol caused concentration-dependent activation of current, and compared with the sham group, its current strength and γ-aminobutyric acid A receptor protein expression decreased. Immunofluorescence revealed that γ-aminobutyric acid type A receptor α2 subunit protein expression decreased and was most obvious at 12 and 15 days after modeling. Our experimental findings confirmed that the γ-aminobutyric acid type A receptor α2 subunit in the chronic constriction injury model rat dorsal root ganglion was downregulated, which may be one of the reasons for the reduction of injury in dorsal root ganglion neurons following muscimol-activated currents.
authors.affiliation: Department of Physiology, Shihezi University School of Medicine, Shihezi 832002, Xinjiang Uygur Autonomous Region, China ; Key Laboratory of Xinjiang Endemic and Ethnic Disease, Shihezi University School of Medicine, Shihezi 832002, Xinjiang Uygur Autonomous Region, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Lian
authors.affiliation: Department of Physiology, Shihezi University School of Medicine, Shihezi 832002, Xinjiang Uygur Autonomous Region, China ; Key Laboratory of Xinjiang Endemic and Ethnic Disease, Shihezi University School of Medicine, Shihezi 832002, Xinjiang Uygur Autonomous Region, China ; Fundamental Medical School of Wuhan University, Wuhan 430071, Hubei Province, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Physiology, Shihezi University School of Medicine, Shihezi 832002, Xinjiang Uygur Autonomous Region, China ; Key Laboratory of Xinjiang Endemic and Ethnic Disease, Shihezi University School of Medicine, Shihezi 832002, Xinjiang Uygur Autonomous Region, China.
authors.forename: Ketao
authors.initials: K
authors.lastname: Ma
authors.affiliation: Department of Physiology, Shihezi University School of Medicine, Shihezi 832002, Xinjiang Uygur Autonomous Region, China ; Key Laboratory of Xinjiang Endemic and Ethnic Disease, Shihezi University School of Medicine, Shihezi 832002, Xinjiang Uygur Autonomous Region, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Zhao
authors.affiliation: Department of Physiology, Shihezi University School of Medicine, Shihezi 832002, Xinjiang Uygur Autonomous Region, China ; Key Laboratory of Xinjiang Endemic and Ethnic Disease, Shihezi University School of Medicine, Shihezi 832002, Xinjiang Uygur Autonomous Region, China.
authors.forename: Zhongshuang
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Department of Physiology, Shihezi University School of Medicine, Shihezi 832002, Xinjiang Uygur Autonomous Region, China ; Key Laboratory of Xinjiang Endemic and Ethnic Disease, Shihezi University School of Medicine, Shihezi 832002, Xinjiang Uygur Autonomous Region, China.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Shang
authors.affiliation: Department of Physiology, Shihezi University School of Medicine, Shihezi 832002, Xinjiang Uygur Autonomous Region, China ; Key Laboratory of Xinjiang Endemic and Ethnic Disease, Shihezi University School of Medicine, Shihezi 832002, Xinjiang Uygur Autonomous Region, China ; Fundamental Medical School of Wuhan University, Wuhan 430071, Hubei Province, China.
authors.forename: Junqiang
authors.initials: J
authors.lastname: Si
authors.affiliation: Department of Physiology, Shihezi University School of Medicine, Shihezi 832002, Xinjiang Uygur Autonomous Region, China ; Key Laboratory of Xinjiang Endemic and Ethnic Disease, Shihezi University School of Medicine, Shihezi 832002, Xinjiang Uygur Autonomous Region, China.
authors.forename: Li
authors.initials: L
authors.lastname: Li
keywords: dorsal root ganglion; immunofluorescence; muscimol; neuropathic pain; paw withdrawal latency; primary afferent depolarization; whole-cell patch clamp; γ-aminobutyric acid; γ-aminobutyric acid type A receptor α2 subunit