title: Histologic evaluation of apical pulp of immature apex following extraction, surface treatment, and replantation in different storage media in dogs.
abstract: OBJECTIVE
  This study was designed to evaluate the apical pulp of open apex teeth following extraction, surface treatment, and preservation in different storage media in dogs.
  
  
  METHODS
  Four healthy male dogs were included in this prospective animal study. In the first phase of extraction, 30 teeth were extracted atraumatically from dogs (incisors and premolars) and then randomly divided into three groups based on different types of storage media: (i) milk, (ii) HBSS, and (iii) dry storage. In milk and HBSS groups, teeth were contaminated by dog's saliva and then kept in milk and HBSS solution, respectively, for 2 h. In the third group, teeth were stored in a dry storage for 2 h. Before replantation, all teeth were soaked in 10% doxycycline. After 2 months, in the second phase of extraction, all experimental teeth were extracted (40 teeth including control group). Three longitudinal sections (3-4 mm thickness) were stained with H&E and investigated under optical microscope. Histologic evaluations were performed, and data were analyzed using Kruskal-Wallis and Fisher's exact test (α = 0.05).
  
  
  RESULTS
  There was a significant difference between the milk and control groups regarding the presence of the odontoblastic layer and resorption (P = 0.04). Also, there was a significant difference between dry storage and control group in the presence of the odontoblastic layer (P = 0.02). There was no statistically significant difference in other histologic factors between different groups (P > 0.05). It was revealed that there is no significant difference between different groups regarding the intensity (P = 0.06) and the type of the inflammation (P = 0.24). Also, the hyperemia showed no significant difference between different groups (P = 0.51).
  
  
  CONCLUSIONS
  It was concluded that HBSS and milk are appropriate storage media. Also, it was shown that 2 h dry storage probably does not threaten the vitality of the pulp cells.
authors.affiliation: Torabinejad Research Center and Pediatrics Department, Isfahan University of Medical Sciences, Isfahan, Iran.
authors.forename: Ebrahim
authors.initials: E
authors.lastname: Jabarifar
authors.forename: Navid
authors.initials: N
authors.lastname: Khalighinejad
authors.forename: Abbas Ali
authors.initials: AA
authors.lastname: Khademi
authors.forename: Seyed Mohammad
authors.initials: SM
authors.lastname: Razavi
authors.forename: Neda
authors.initials: N
authors.lastname: Birjandi
authors.forename: Hamid
authors.initials: H
authors.lastname: Badrian
authors.forename: Ghasem
authors.initials: G
authors.lastname: Ansari
mesh_terms: D000818:Animals; D001641:Bicuspid; D003782:Dental Pulp / Q000473:pathology*; D004285:Dogs; D007180:Incisor; D008297:Male; D019188:Organ Preservation Solutions / Q000737:chemistry*; D011446:Prospective Studies; D013194:Staining and Labeling; D014021:Tissue Preservation / Q000379:methods*; D014081:Tooth Extraction; D014090:Tooth Replantation
chemical_list: D019188:Organ Preservation Solutions
keywords: apical pulp; avulsion; open apex tooth; storage media