title: Steroidal alkaloids from the marine sponge Corticium niger that inhibit growth of human colon carcinoma cells.
abstract: Bioinformatic analysis of data from the NCI-60 cell cytotoxicity screen revealed a subset of extracts that showed selective cytotoxic activity toward human colon carcinoma cell lines. Bioassay-guided fractionation of a colon cancer selective extract from a Philippines collection of the marine sponge Corticium niger provided two new steroidal alkaloids, plakinamines N (1) and O (2), along with two known compounds of the plakinamine class (3, 4). The structures of these compounds were elucidated by interpretation of combined MS and NMR spectroscopic data. Plakinamines N (1), O (2), and J (4) were tested for antiproliferative activity in the NCI-60 screen, and they showed enhanced inhibitory effects against all of the colon cell lines with mean GI50 values of 11.5, 2.4, and 1.4 μM, respectively.
authors.affiliation: Molecular Targets Laboratory, Center for Cancer Research, National Cancer Institute , Frederick, Maryland 21702-1201, United States.
authors.forename: Suthananda N
authors.initials: SN
authors.lastname: Sunassee
authors.forename: Tanya
authors.initials: T
authors.lastname: Ransom
authors.forename: Curtis J
authors.initials: CJ
authors.lastname: Henrich
authors.forename: John A
authors.initials: JA
authors.lastname: Beutler
authors.forename: David G
authors.initials: DG
authors.lastname: Covell
authors.forename: James B
authors.initials: JB
authors.lastname: McMahon
authors.forename: Kirk R
authors.initials: KR
authors.lastname: Gustafson
mesh_terms: D000470:Alkaloids / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D000818:Animals; D003110:Colonic Neoplasms / Q000188:drug therapy; D004354:Drug Screening Assays, Antitumor; D006801:Humans; D008386:Marine Biology; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D010679:Philippines; D011161:Porifera / Q000737:chemistry*; D013256:Steroids / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*
chemical_list: D000470:Alkaloids; D013256:Steroids; C482751:plakinamine J; C000599237:plakinamine N; C000599239:plakinamine O