title: Selective silencing of 2Cys and type-IIB Peroxiredoxins discloses their roles in cell redox state and stress signaling.
abstract: Peroxiredoxins (Prx) catalyse the reduction of hydrogen peroxide (H2O2) and, in association with catalases and other peroxidases, may participate in signal transduction by regulating intercellular H2O2 concentration that in turn can control gene transcription and cell signaling. Using virus-induced-gene-silencing (VIGS), 2-Cys Peroxiredoxin (2CysPrx) family and type-II Peroxiredoxin B (PrxIIB) gene were silenced in Nicotiana benthamiana, to study the impact that the loss of function of each Prx would have in the antioxidant system under control (22 °C) and severe heat stress conditions (48 °C). The results showed that both Prxs, although in different organelles, influence the regeneration of ascorbate to a significant extent, but with different purposes. 2CysPrx affects abscisic acid (ABA) biosynthesis through ascorbate, while PrxIIB does it probably through the xanthophyll cycle. Moreover, 2CysPrx is key in H2O2 scavenging and in consequence in the regulation of ABA signaling downstream of reactive oxygen species and PrxIIB provides an important assistance for H2O2 peroxisome scavenges.
authors.affiliation: Departamento de Recursos Naturais, Ambiente e Território (DRAT)/Centro de Botânica Aplicada à Agricultura (CBAA), Institute of Agronomy, University of Lisbon, Tapada da Ajuda, 1349-017, Lisbon, Portugal.
authors.forename: Patrícia
authors.initials: P
authors.lastname: Vidigal
authors.affiliation: Institut de Recerca i Tecnologia Agroalimentàries (IRTA), Centre for Research in Agricultural Genomics (CSIC-IRTA-UAB-UB) Campus Universitat Autonoma de Barcelona (UAB), Edificio Center for Research in Agricultural Genomics (CRAG), Bellaterra, Cerdanyola del Vallès, 08193, Barcelona, Spain.
authors.forename: Ana Montserrat
authors.initials: AM
authors.lastname: Martin-Hernandez
authors.affiliation: Institut de Recerca i Tecnologia Agroalimentàries (IRTA), Centre for Research in Agricultural Genomics (CSIC-IRTA-UAB-UB) Campus Universitat Autonoma de Barcelona (UAB), Edificio Center for Research in Agricultural Genomics (CRAG), Bellaterra, Cerdanyola del Vallès, 08193, Barcelona, Spain.
authors.forename: Cèlia
authors.initials: C
authors.lastname: Guiu-Aragonés
authors.affiliation: Departamento de Recursos Naturais, Ambiente e Território (DRAT)/Centro de Botânica Aplicada à Agricultura (CBAA), Institute of Agronomy, University of Lisbon, Tapada da Ajuda, 1349-017, Lisbon, Portugal.
authors.forename: Sara
authors.initials: S
authors.lastname: Amâncio
authors.affiliation: Departamento de Recursos Naturais, Ambiente e Território (DRAT)/Centro de Botânica Aplicada à Agricultura (CBAA), Institute of Agronomy, University of Lisbon, Tapada da Ajuda, 1349-017, Lisbon, Portugal.
authors.forename: Luísa
authors.initials: L
authors.lastname: Carvalho
mesh_terms: D000040:Abscisic Acid / Q000378:metabolism; D000872:Anthocyanins / Q000378:metabolism; D053898:Biosynthetic Pathways / Q000235:genetics; D002338:Carotenoids / Q000378:metabolism; D002734:Chlorophyll / Q000378:metabolism; D002736:Chloroplasts / Q000235:genetics; D003545:Cysteine / Q000378:metabolism*; D003600:Cytosol / Q000378:metabolism; D005453:Fluorescence; D018506:Gene Expression Regulation, Plant; D020868:Gene Silencing; D017343:Genes, Plant; D005978:Glutathione / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000378:metabolism; D009249:NADP / Q000378:metabolism; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000235:genetics; D054464:Peroxiredoxins / Q000235:genetics* / Q000378:metabolism; D010641:Phenotype; D010749:Phosphoprotein Phosphatases / Q000235:genetics / Q000378:metabolism; D045332:Photosystem II Protein Complex / Q000378:metabolism; D018515:Plant Leaves / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D015398:Signal Transduction / Q000235:genetics; D013312:Stress, Physiological / Q000235:genetics; D014026:Nicotiana / Q000235:genetics / Q000378:metabolism*
chemical_list: D000872:Anthocyanins; D045332:Photosystem II Protein Complex; D002734:Chlorophyll; D002338:Carotenoids; D009249:NADP; D000040:Abscisic Acid; D006861:Hydrogen Peroxide; D054464:Peroxiredoxins; D010749:Phosphoprotein Phosphatases; D005978:Glutathione; D003545:Cysteine
keywords: 2-Cys Peroxiredoxin; abscisic acid; ascorbate; heat stress; hydrogen peroxide; peroxisome; type-II Peroxiredoxin B