title: Chemotactic activity of gestational tissues through late pregnancy, term labor, and RU486-induced preterm labor in Guinea pigs.
abstract: OBJECTIVE
  Is increased leukocyte chemotactic activity (CA) from gestational tissues necessary for term or preterm labor in guinea pigs?
  
  
  METHODS
  Tissue extracts were prepared from pregnant guinea pig decidua-myometrium, cervix, fetal membranes (amniochorion), and placenta during early third trimester (n = 8), term not in labor (TNL, n = 5), and term spontaneous labor (TL, n = 6), RU486-induced preterm labor (PTL, n = 6), or controls (cPTL, n = 5). Leukocyte CA was assessed using a modified Boyden chamber assay. Extract chemokine and maternal progesterone concentrations were quantified by enzyme immunoassay.
  
  
  RESULTS
  Only the extracts from amniochorion demonstrated increased CA through late gestation and labor. In contrast, CA was decreased in extracts from amniochorion and cervix from animals after RU486-induced PTL. Maternal progesterone concentrations remained high in all groups.
  
  
  CONCLUSIONS
  Leukocyte CA of intrauterine tissues is increased in term spontaneous labor. However, RU486-induced preterm labor occurs in the absence of increased CA.
authors.affiliation: Department of Obstetrics and Gynecology, Perinatology Research Branch, Wayne State University, Detroit, MI, USA.
authors.forename: Nardhy
authors.initials: N
authors.lastname: Gomez-Lopez
authors.forename: Wing-Chiu
authors.initials: WC
authors.lastname: Tong
authors.forename: Marcia
authors.initials: M
authors.lastname: Arenas-Hernandez
authors.forename: Satomi
authors.initials: S
authors.lastname: Tanaka
authors.forename: Omar
authors.initials: O
authors.lastname: Hajar
authors.forename: David M
authors.initials: DM
authors.lastname: Olson
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Taggart
authors.forename: Bryan F
authors.initials: BF
authors.lastname: Mitchell
mesh_terms: D000653:Amniotic Fluid / Q000187:drug effects / Q000378:metabolism; D000818:Animals; D003656:Decidua / Q000187:drug effects / Q000378:metabolism / Q000502:physiology; D005321:Extraembryonic Membranes / Q000187:drug effects / Q000378:metabolism / Q000502:physiology; D005260:Female; D005322:Premature Rupture of Fetal Membranes / Q000139:chemically induced / Q000378:metabolism / Q000503:physiopathology; D005865:Gestational Age; D006168:Guinea Pigs; D007962:Leukocytes / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D015735:Mifepristone / Q000494:pharmacology*; D009215:Myometrium / Q000187:drug effects / Q000378:metabolism / Q000502:physiology; D007752:Obstetric Labor, Premature / Q000139:chemically induced* / Q000378:metabolism / Q000503:physiopathology*; D010920:Placenta / Q000187:drug effects / Q000378:metabolism / Q000502:physiology; D011247:Pregnancy; D011263:Pregnancy Trimester, Third / Q000187:drug effects / Q000378:metabolism / Q000502:physiology; D011374:Progesterone / Q000378:metabolism; D047929:Term Birth / Q000378:metabolism / Q000502:physiology*
chemical_list: D015735:Mifepristone; D011374:Progesterone
keywords: Chemotaxis; decidua; fetal membranes; myometrium; parturition