title: Quantitative gene expression analysis of some sodium ion transporters under salinity stress in Aeluropus littoralis.
abstract: Plant sodium transporters activity is one of the most important salt tolerance mechanisms to keep normal status of cytosolic sodium content. In the present study, expression pattern of genes encoding Na(+)/H(+) antiporters in the plasma membrane (SOS1 gene), vacuolar membrane (NHX1 gene) and H(+)-ATPase pump (VHA gene) in Aeluropus littoralis under different treatments of NaCl was measured by the semi-quantitative RT-PCR method. Our results indicated that root and shoot sodium contents were increased along with increasing salinity pressure. In response to 200 and 400 mM NaCl, mRNA level of SOS1 and NHX1 was increased in the shoot and root tissues, while VHA transcripts were increased only under 400 mM of NaCl. Transcripts of VHA-c and NHX1 in root were higher than shoot in all treatments. In general, our results indicated that transcriptional level of SOS1, and NHX1 genes induced in parallel with VHA expression may be involved in controlling cytosolic Na(+) concentration in A. littoralis.
authors.affiliation: Department of Agronomy and Plant Breeding, Agricultural Sciences and Natural Resources University of Sari, Sari, Iran.
authors.forename: Masoumeh
authors.initials: M
authors.lastname: Rezaei Moshaei
authors.affiliation: Department of Agronomy and Plant Breeding, Agricultural Sciences and Natural Resources University of Sari, Sari, Iran.
authors.forename: Ghorban Ali
authors.initials: GA
authors.lastname: Nematzadeh
authors.affiliation: Department of Biotechnology, Faculty of Energy Engineering and New Technologies, Shahid Beheshti University, Tehran, Iran.
authors.forename: Hossein
authors.initials: H
authors.lastname: Askari
authors.affiliation: Young Researchers and Elite Club, Sari Branch, Islamic Azad University, Sari, Iran.
authors.forename: Amir Sasan
authors.initials: AS
authors.lastname: Mozaffari Nejad
authors.affiliation: Genetics and Agricultural Biotechnology Institute of Tabarestan, Agricultural Sciences and Natural Resources University of Sari, Sari, Iran.
authors.forename: Ali
authors.initials: A
authors.lastname: Pakdin
keywords: H+-ATPase pump; NHX1; SOS1; Salt stress; Semi-quantitative RT-PCR