title: Rapid differentiation of in vitro cellular responses to toxic chemicals by using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.
abstract: Changes in protein expression as a cellular response to chemical exposure have been well established. Current methods for monitoring cellular responses usually require the use of specific reagents and/or labor-intensive procedures. The present study demonstrates the concept of using mass spectral pattern to distinguish different cellular responses. The concept is based on the ability to acquire a unique mass spectral pattern directly from a specific cell culture by using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). The results demonstrate that distinguishable and reproducible spectral patterns can be obtained from different cellular responses.
authors.affiliation: Department of Chemistry and Biochemistry, University of North Carolina at Greensboro, Greensboro, North Carolina, USA; Department of Nanoscience, Joint School of Nanoscience and Nanoengineering, Greensboro, North Carolina, USA.
authors.forename: Norman H L
authors.initials: NH
authors.lastname: Chiu
authors.forename: Zhenquan
authors.initials: Z
authors.lastname: Jia
authors.forename: Reynaldo
authors.initials: R
authors.lastname: Diaz
authors.forename: Petra
authors.initials: P
authors.lastname: Wright
mesh_terms: D016604:Aflatoxin B1 / Q000633:toxicity*; D002460:Cell Line; D006801:Humans; D006861:Hydrogen Peroxide / Q000633:toxicity*; D025341:Principal Component Analysis; D020543:Proteome / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D020543:Proteome; D016604:Aflatoxin B1; D006861:Hydrogen Peroxide
keywords: Analytical chemistry; Hazard/risk assessment; Risk assessment