title: Inflammatory breast cancer: high incidence of detection of mixed human cytomegalovirus genotypes associated with disease pathogenesis.
abstract: Inflammatory breast cancer (IBC) is a highly metastatic, aggressive, and fatal form of breast cancer. Patients presenting with IBC are characterized by a high number of axillary lymph node metastases. Recently, we found that IBC carcinoma tissues contain significantly higher levels of human cytomegalovirus (HCMV) DNA compared to other breast cancer tissues that may regulate cell signaling pathways. In fact, HCMV pathogenesis and clinical outcome can be statistically associated with multiple HCMV genotypes within IBC. Thus, in the present study, we established the incidence and types of HCMV genotypes present in carcinoma tissues of infected non-IBC versus IBC patients. We also assessed the correlation between detection of mixed genotypes of HCMV and disease progression. Genotyping of HCMV in carcinoma tissues revealed that glycoprotein B (gB)-1 and glycoprotein N (gN)-1 were the most prevalent HCMV genotypes in both non-IBC and IBC patients with no significant difference between patients groups. IBC carcinoma tissues, however, showed statistically significant higher incidence of detection of the gN-3b genotype compared to non-IBC patients. The incidence of detection of mixed genotypes of gB showed that gB-1 + gB-3 was statistically significantly higher in IBC than non-IBC patients. Similarly, the incidence of detection of mixed genotypes of gN showed that gN-1 + gN-3b and gN-3 + gN-4b/c were statistically significant higher in the carcinoma tissues of IBC than non-IBC. Mixed presence of different HCMV genotypes was found to be significantly correlated with the number of metastatic lymph nodes in non-IBC but not in IBC patients. In IBC, detection of mixed HCMV different genotypes significantly correlates with lymphovascular invasion and formation of dermal lymphatic emboli, which was not found in non-IBC patients.
authors.affiliation: Department of Zoology, Faculty of Science, Cairo University , Giza , Egypt.
authors.forename: Hossam Taha
authors.initials: HT
authors.lastname: Mohamed
authors.affiliation: Department of General Surgery, Faculty of Medicine, Ain Shams University , Cairo , Egypt.
authors.forename: Mohamed
authors.initials: M
authors.lastname: El-Shinawi
authors.affiliation: Department of Pathology, National Cancer Institute, Cairo University , Giza , Egypt.
authors.forename: M Akram
authors.initials: MA
authors.lastname: Nouh
authors.affiliation: Department of Zoology, Faculty of Science, Cairo University , Giza , Egypt.
authors.forename: Abdel-Rahman
authors.initials: AR
authors.lastname: Bashtar
authors.affiliation: Department of Botany, Faculty of Science, Cairo University , Giza , Egypt.
authors.forename: Elsayed Tarek
authors.initials: ET
authors.lastname: Elsayed
authors.affiliation: Department of Microbiology, School of Medicine, New York University , New York, NY , USA.
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Schneider
authors.affiliation: Department of Zoology, Faculty of Science, Cairo University , Giza , Egypt.
authors.forename: Mona Mostafa
authors.initials: MM
authors.lastname: Mohamed
keywords: UL55; UL73; glycoproteins; human cytomegalovirus; inflammatory breast neoplasms; lymphovascular invasion; metastasis