title: A comprehensive insight into the lipid composition of Myxococcus xanthus by UPLC-ESI-MS.
abstract: Analysis of whole cell lipid extracts of bacteria by means of ultra-performance (UP)LC-MS allows a comprehensive determination of the lipid molecular species present in the respective organism. The data allow conclusions on its metabolic potential as well as the creation of lipid profiles, which visualize the organism's response to changes in internal and external conditions. Herein, we describe: i) a fast reversed phase UPLC-ESI-MS method suitable for detection and determination of individual lipids from whole cell lipid extracts of all polarities ranging from monoacylglycerophosphoethanolamines to TGs; ii) the first overview of a wide range of lipid molecular species in vegetative Myxococcus xanthus DK1622 cells; iii) changes in their relative composition in selected mutants impaired in the biosynthesis of α-hydroxylated FAs, sphingolipids, and ether lipids; and iv) the first report of ceramide phosphoinositols in M. xanthus, a lipid species previously found only in eukaryotes.
authors.affiliation: Merck Stiftungsprofessur für Molekulare Biotechnologie, Fachbereich Biowissenschaften, Johann Wolfgang Goethe-Universität Frankfurt, D-60438 Frankfurt am Main, Germany.
authors.forename: Wolfram
authors.initials: W
authors.lastname: Lorenzen
authors.affiliation: Merck Stiftungsprofessur für Molekulare Biotechnologie, Fachbereich Biowissenschaften, Johann Wolfgang Goethe-Universität Frankfurt, D-60438 Frankfurt am Main, Germany.
authors.forename: Kenan A J
authors.initials: KA
authors.lastname: Bozhüyük
authors.affiliation: Cluster of Excellence Macromolecular Complexes, Johann Wolfgang Goethe-Universität Frankfurt, D-60438 Frankfurt am Main, Germany Buchmann Institute for Molecular Life Sciences (BMLS), Johann Wolfgang Goethe-Universität Frankfurt, D-60438 Frankfurt am Main, Germany.
authors.forename: Niña S
authors.initials: NS
authors.lastname: Cortina
authors.affiliation: Merck Stiftungsprofessur für Molekulare Biotechnologie, Fachbereich Biowissenschaften, Johann Wolfgang Goethe-Universität Frankfurt, D-60438 Frankfurt am Main, Germany Buchmann Institute for Molecular Life Sciences (BMLS), Johann Wolfgang Goethe-Universität Frankfurt, D-60438 Frankfurt am Main, Germany.
authors.forename: Helge B
authors.initials: HB
authors.lastname: Bode
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics; D002851:Chromatography, High Pressure Liquid; D056148:Chromatography, Reverse-Phase; D062126:Databases, Chemical; D008401:Gas Chromatography-Mass Spectrometry; D050356:Lipid Metabolism; D008055:Lipids / Q000032:analysis* / Q000737:chemistry; D055432:Metabolomics / Q000379:methods; D015394:Molecular Structure; D009154:Mutation; D016941:Myxococcus xanthus / Q000378:metabolism*; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry
chemical_list: D001426:Bacterial Proteins; D008055:Lipids
keywords: ceramide phosphoinositols; electrospray ionization-mass spectrometry; ether lipids; lipid profiles; lipidomics; myxobacteria; reversed phase; ultra-performance liquid chromatography