title: Wnt5A expression is associated with the tumor metastasis and clinical survival in cervical cancer.
abstract: OBJECTIVE
  To identify the clinical significance of Wnt5A expression in the development and progression of cervical cancer.
  
  
  METHODS
  Real-time PCR was performed in 8 pairs of surgically resected cervical cancer and adjacent normal cervical tissues. Immunohistochemistry was performed to examine Wnt5A expression in 94 paraffin-embedded cervical cancer samples. Associations of Wnt5A expression with clinicopathological factors and clinical survival were analyzed.
  
  
  RESULTS
  Wnt5A expression was overexpressed in cervical cancer tissues compared with adjacent normal cervix. Wnt5A expression tended to be positively correlated with lymph nodes metastasis (P = 0.028) and recurrence (P = 0.009). Moreover, patients with higher Wnt5A expression in cancer tissues had better overall (P = 0.004) and recurrent-free survival (P = 0.012) than those with lower Wnt5A expression. Multivariate analysis revealed that Wnt5A was an independent prognostic factor (P = 0.026) for predicting overall survival of cervical cancer patients.
  
  
  CONCLUSIONS
  Upregulation of Wnt5A was associated with metastasis and progression of cervical cancer. The results of our study unravel the significance of Wnt/Ca2+ signaling in cervical cancer.
authors.affiliation: Department of Obstetrics and Gynecology, Shenzhen Second People's Hospital, The First Affiliated Hospital of Shenzhen University Shenzhen 518000, China.
authors.forename: Li
authors.initials: L
authors.lastname: Lin
authors.affiliation: Department of Obstetrics and Gynecology, Guangzhou Women and Children's Medical Center Guangzhou 510120, China.
authors.forename: Yaqiong
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Obstetrics and Gynecology, Shenzhen Second People's Hospital, The First Affiliated Hospital of Shenzhen University Shenzhen 518000, China.
authors.forename: Weihua
authors.initials: W
authors.lastname: Zhao
authors.affiliation: Department of Obstetrics and Gynecology, Shenzhen Second People's Hospital, The First Affiliated Hospital of Shenzhen University Shenzhen 518000, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Sun
authors.affiliation: Department of Obstetrics and Gynecology, Shenzhen Second People's Hospital, The First Affiliated Hospital of Shenzhen University Shenzhen 518000, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Chen
mesh_terms: D014408:Biomarkers, Tumor / Q000032:analysis* / Q000235:genetics; D016009:Chi-Square Distribution; D018572:Disease-Free Survival; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D053208:Kaplan-Meier Estimate; D008875:Middle Aged; D015999:Multivariate Analysis; D009362:Neoplasm Metastasis; D009364:Neoplasm Recurrence, Local; D011237:Predictive Value of Tests; D011518:Proto-Oncogene Proteins / Q000032:analysis* / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis; D060888:Real-Time Polymerase Chain Reaction; D012307:Risk Factors; D013997:Time Factors; D015854:Up-Regulation; D002583:Uterine Cervical Neoplasms / Q000737:chemistry* / Q000235:genetics / Q000401:mortality / Q000473:pathology / Q000628:therapy; D051153:Wnt Proteins / Q000032:analysis* / Q000235:genetics; D000071818:Wnt-5a Protein
chemical_list: D014408:Biomarkers, Tumor; D011518:Proto-Oncogene Proteins; D012333:RNA, Messenger; C085389:WNT5A protein, human; D051153:Wnt Proteins; D000071818:Wnt-5a Protein
keywords: Cervical cancer; Wnt/Ca2+ signaling; Wnt5A; prognosis