title: [Polymorphisms of RHCE gene among serologic RhD negative donors of Han population in Chinese Jiangsu area].
abstract: This study was purposed to investigate the RHCE gene polymorphisms in Chinese Jiangsu Han blood donors with and without RHD gene among serologic RhD negative population. PCR with sequence-specific primers (PCR-SSP) was used to detect RHCE genotype in 337 serologic RhD negative, RHD gene positive donors. The RHD gene-specific polymorphisms were also determined by PCR-SSP in these donors. The results showed that among 337 serologic RhD negative, RHD gene positive donor 20 were RHCE*C/C, 62 RHCE*C/c, 24 RHCE*c/c, 25 RHCE*E/e, and 81 RHCE*e/e; the allele frequencies for RHCE*C and RHCE*c were 0.4811 and 0.5189, respectively; and for RHCE*E and RHCE*e 0.1179 and 0.8821. Among 231 RHD gene negative donors, 3 were RHCE*C/C, 34 RHCE*C/c, 194 RHCE*c/c, 15 RHCE*E/e, 216 RHCE*e/e; the allele frequencies for RHCE*C and RHCE*c were 0.0866 and 0.9134, respectively; and allele frequencies for RHCE*E and RHCE*e were 0.0325 and 0.9675, respectively. It is concluded that the most prevalent allele of RHCE gene was RHCE*ccee in Chinese Han Jiangsu RHD gene negative population. There is statistical difference in RHCE genotype distribution among serologic RhD negative population with and without RHD gene.
authors.affiliation: Jiangsu Province Blood Center, Nanjing 210042, Jiangsu Province, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Chen
authors.affiliation: Jiangsu Province Blood Center, Nanjing 210042, Jiangsu Province, China.
authors.forename: Jian-Yu
authors.initials: JY
authors.lastname: Xiao
authors.affiliation: Wuxi Red Cross Blood Center, Wuxi 214021, Jiangsu Province, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Yu
authors.affiliation: Jiangsu Province Blood Center, Nanjing 210042, Jiangsu Province, China.
authors.forename: Cheng-Yin
authors.initials: CY
authors.lastname: Huang
authors.affiliation: Department of Rheumatology and Imunology, The Affiliated Drum Tower Hospital of Nanjing University Medical School, Nanjing 210008, Jiangsu Province, China. E-mail: yaogenhong@126.com.
authors.forename: Gen-Hong
authors.initials: GH
authors.lastname: Yao
mesh_terms: D000483:Alleles; D044466:Asian People / Q000235:genetics*; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012204:Rh-Hr Blood-Group System / Q000235:genetics*
chemical_list: C066468:RHCE protein, human; D012204:Rh-Hr Blood-Group System; C026410:Rho(D) antigen