title: Effects of hydroxysafflor yellow A on proliferation and collagen synthesis of rat vascular adventitial fibroblasts induced by angiotensin II.
abstract: 1) examine the effects of hydroxysafflor yellow A (HSYA) on the proliferation, collagen and cytokine synthesis of vascular adventitial fibroblasts as induced by angiotensin II (Ang II) in normal Sprague-Dawley (SD) rats in vitro, and 2) to assess the effects of HSYA on morphological changes and collagen accumulation of vascular adventitia in spontaneously hypertensive rats (SHR) in vivo. In vitro experiment, vascular adventitial fibroblasts from SD rats were isolated, cultured, and divided into control groups, model groups and HSYA groups. Cell morphology of adventitial fibroblasts was assessed using laser confocal microscopy, while cell proliferation with the MTT assay, and collagen synthesis was determined using hydroxyproline chromatometry. Immunocytochemistry and reverse transcription PCR were used for detecting the expression of TGF-β1, MMP-1, α-SMA and NF-κB in adventitial fibroblasts. In vivo experiment, vascular adventitia proliferation and collagen synthesis were analyzed using hematoxylin-eosin and Sirius staining. Our results showed that: 1) in vitro experiment of SD rats, HSYA inhibited proliferative activity and collagen synthesis of adventitial fibroblasts as induced by Ang II, and the inhibitory effects of HSYA on the increased expression of MMP-1, TGF-β1, α-SMA and NF-κB p65 as induced by Ang II were assessed, and 2) in vivo experiment of SHR, histological analysis displayed fewer pathological changes of vascular adventitia in HSYA treatment groups as compared with no HSYA treatment groups, and MMP-1, TGF-β1, α-SMA and NF-κB p65 expression significantly reduced after HSYA treatment (P < 0.05). Our results revealed that HSYA treatment significantly decreased the amount of cytokines and collagen synthesis in vascular adventitia components. This study provides experimental evidence demonstrating that HSYA has the capacity to decrease vascular adventitia proliferation and hyperplasia during vascular remodeling.
authors.affiliation: College of Basic Medicine, Binzhou Medical University Yantai, China.
authors.forename: Wendan
authors.initials: W
authors.lastname: Yuan
authors.affiliation: College of Basic Medicine, Binzhou Medical University Yantai, China.
authors.forename: Dongxia
authors.initials: D
authors.lastname: Yang
authors.affiliation: College of Basic Medicine, Binzhou Medical University Yantai, China.
authors.forename: Xuhong
authors.initials: X
authors.lastname: Sun
authors.affiliation: College of Basic Medicine, Binzhou Medical University Yantai, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.affiliation: College of Basic Medicine, Binzhou Medical University Yantai, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Wang
authors.affiliation: College of Basic Medicine, Binzhou Medical University Yantai, China.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Ophthalmology, Yuhuangding Hospital Yantai, China.
authors.forename: Xuejing
authors.initials: X
authors.lastname: Man
authors.affiliation: College of Basic Medicine, Binzhou Medical University Yantai, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Fu
mesh_terms: D000199:Actins / Q000235:genetics / Q000378:metabolism; D063194:Adventitia / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D000804:Angiotensin II / Q000494:pharmacology*; D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D002599:Chalcone / Q000031:analogs & derivatives* / Q000494:pharmacology; D003094:Collagen / Q000096:biosynthesis*; D016207:Cytokines / Q000235:genetics / Q000378:metabolism; D005347:Fibroblasts / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D005786:Gene Expression Regulation; D008297:Male; D020781:Matrix Metalloproteinase 1 / Q000235:genetics / Q000378:metabolism; D016328:NF-kappa B / Q000235:genetics / Q000378:metabolism; D011809:Quinones / Q000494:pharmacology*; D012333:RNA, Messenger / Q000378:metabolism; D017207:Rats, Sprague-Dawley; D015398:Signal Transduction / Q000187:drug effects; D013997:Time Factors; D053773:Transforming Growth Factor beta1 / Q000235:genetics / Q000378:metabolism; D066253:Vascular Remodeling / Q000187:drug effects*
chemical_list: D000199:Actins; D000804:Angiotensin II; D002599:Chalcone; D003094:Collagen; D016207:Cytokines; D020781:Matrix Metalloproteinase 1; D016328:NF-kappa B; D011809:Quinones; D012333:RNA, Messenger; D053773:Transforming Growth Factor beta1; C508962:Tgfb1 protein, rat; C498862:Acta2 protein, rat; C085278:hydroxysafflor yellow A; C576840:MMP1 protein, rat
keywords: Hydroxysafflor yellow A; adventitial fibroblasts; angiotensin II; collagen; proliferation