title: Characterization of molecularly imprinted polymer nanoparticles by photon correlation spectroscopy.
abstract: We follow template-binding induced aggregation of nanoparticles enantioselectively imprinted against (S)-propranolol, and the non-imprinted ones, using photon correlation spectroscopy (dynamic light scattering). The method requires no separation steps. We have characterized binding of (R,S)-propranolol to the imprinted polymers and determined the degree of non-specificity by comparing the specific binding with the results obtained using non-imprinted nanoparticles. Using (S)-propranolol as a template for binding to (S)-imprinted nanoparticle, and (R)-propranolol as a non-specific control, we have determined range of concentrations where chiral recognition can be observed. By studying aggregation induced by three analytes related to propranolol, atenolol, betaxolol, and 1-amino-3-(naphthalen-1-yloxy)propan-2-ol, we were able to determine which parts of the template are involved in the specific binding, discuss several details of specific adsorption, and the structure of the imprinted site.
authors.affiliation: Acreo Swedish ICT AB, Arvid Hedvallsbacke 4, 41133, Göteborg, Sweden; Biothema AB, Handens stationsväg 17, 13640, Handen, Sweden.
authors.forename: Björn
authors.initials: B
authors.lastname: Malm
authors.forename: Keiichi
authors.initials: K
authors.lastname: Yoshimatsu
authors.forename: Lei
authors.initials: L
authors.lastname: Ye
authors.forename: Anatol
authors.initials: A
authors.lastname: Krozer
mesh_terms: D054802:Molecular Imprinting / Q000379:methods*; D053758:Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D017785:Photons; D011108:Polymers / Q000737:chemistry*; D011433:Propranolol / Q000737:chemistry; D013057:Spectrum Analysis; D013237:Stereoisomerism
chemical_list: D011108:Polymers; D011433:Propranolol
keywords: Brownian motion; aggregation kinetics; chiral selectivity; colloidal suspension; molecularly imprinted polymers