title: α-Klotho is an acute phase protein and altered by restraint stress in mice.
abstract: OBJECTIVE
  To identify whether α-Klotho is a kind of acute phase protein and alternation in the level of protein and mRNA under restraint stress.
  
  
  METHODS
  48 mice were divided into three groups of 16: 1h, 10h and 20 h treat time group. Each group included restraint stress and control subgroup with same number animals (n = 8). ELISA was utilized in the assay of serum α-Klotho and corticosterone, RT-PCR was occupied in the detection of the expression of α-Klotho mRNA in renal tissue.
  
  
  RESULTS
  α-Klotho protein concentration of control group 1h, 10h and 20 h was 757.71 ± 333.93 pg/ml, 687.38 ± 342.79 pg/ml and 912.90 ± 337.8 pg/ml, respectively. While the concentration of restraint stress group 1h, 10h and 20 h was 726.40 ± 342.79 pg/ml, 1261.54 ± 442.71 pg/ml, and 1696.18 ± 404.11 pg/ml. There was no significant difference among 1h, 10h and 20 h control groups (P > 0.05) as well as 1h treat time subgroup. Compared with respective control group, the difference of restraint stress group in 10h and 20 h treat time group was significant (P < 0.05). The expression of α-Klotho mRNA was slightly downregulated even when the mice underwent a 1 hour restraint stress, though without significance (p > 0.05). Prominent fold change, 2.02 and 2.46, happened in 10h and 20 h restraint group with significance (P < 0.05 and P < 0.01), respectively.
  
  
  CONCLUSIONS
  α-Klotho is a kind of acute phase protein. The serum α-Klotho protein is promoted while the α-Klotho mRNA is downregulated under the constraint stress.
authors.affiliation: Department of Geriatric Cardiology, The First Affiliated Hospital of Medical College, Xi'an Jiaotong University Xi'an 710061, China.
authors.forename: Xian-Ming
authors.initials: XM
authors.lastname: Su
authors.affiliation: Department of Geriatric Cardiology, The First Affiliated Hospital of Medical College, Xi'an Jiaotong University Xi'an 710061, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Yang
mesh_terms: D000209:Acute-Phase Proteins / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D015415:Biomarkers / Q000097:blood; D003345:Corticosterone / Q000097:blood; D004195:Disease Models, Animal; D005966:Glucuronidase / Q000097:blood* / Q000235:genetics; D000090265:Klotho Proteins; D008297:Male; D008810:Mice, Inbred C57BL; D012333:RNA, Messenger / Q000097:blood; D012149:Restraint, Physical; D013315:Stress, Psychological / Q000097:blood* / Q000209:etiology / Q000235:genetics; D013997:Time Factors; D015854:Up-Regulation
chemical_list: D000209:Acute-Phase Proteins; D015415:Biomarkers; D012333:RNA, Messenger; D005966:Glucuronidase; D000090265:Klotho Proteins; D003345:Corticosterone
keywords: acute phase protein (APP); corticosterone; restraint stress; α-Klotho