title: Stable Heliconius butterfly hybrid zones are correlated with a local rainfall peak at the edge of the Amazon basin.
abstract: Multilocus clines between Müllerian mimetic races of Heliconius butterflies provide a classic example of the maintenance of hybrid zones and their importance in speciation. Concordant hybrid zones in the mimics Heliconius erato and H. melpomene in northern Peru were carefully documented in the 1980s, and this prior work now permits a historical analysis of the movement or stasis of the zones. Previous work predicted that these zones might be moving toward the Andes due to selective asymmetry. Extensive deforestation and climate change might also be expected to affect the positions and widths of the hybrid zones. We show that the positions and shapes of these hybrid zones have instead remained remarkably stable between 1985 and 2012. The stability of this interaction strongly implicates continued selection, rather than neutral mixing following secondary contact. The stability of cline widths and strong linkage disequilibria (gametic correlation coefficients Rmax = 0.35-0.56 among unlinked loci) over 25 years suggest that mimetic selection pressures on each color pattern locus have remained approximately constant (s ≈ 0.13-0.40 per locus in both species). Exceptionally high levels of precipitation at the edge of the easternmost Andes may act as a population density trough for butterflies, trapping the hybrid zones at the foot of the mountains, and preventing movement. As such, our results falsify one prediction of the Pleistocene Refugium theory: That the ranges of divergent species or subspecies should be centered on regions characterized by maxima of rainfall, with hybrid zones falling in more arid regions between them.
authors.affiliation: Department of Genetics, Evolution and Environment, University College London, Gower Street, London, WC1E 6BT, United Kingdom; Department of Biology, University of York, Wentworth Way, York, YO10 5DD, United Kingdom. neil.rosser@york.ac.uk.
authors.forename: Neil
authors.initials: N
authors.lastname: Rosser
authors.forename: Kanchon K
authors.initials: KK
authors.lastname: Dasmahapatra
authors.forename: James
authors.initials: J
authors.lastname: Mallet
mesh_terms: D000818:Animals; D002080:Butterflies / Q000235:genetics*; D049810:Genetic Speciation; D006824:Hybridization, Genetic; D015810:Linkage Disequilibrium; D011891:Rain; D012621:Seasons; D012641:Selection, Genetic
keywords: Frequency-dependent selection; Heliconius butterflies; Pleistocene refugia; mimicry; moving hybrid zones