title: Microsatellite primer development for post oak, Quercus stellata (Fagaceae).
abstract: OBJECTIVE
  The American Cross Timbers forest ecosystem runs from southeastern Kansas to Central Texas and is primarily composed of post oak (Quercus stellata). This old-growth forest currently occupies only about 2% of its ancestral range. To facilitate genetic research on this species, we developed microsatellite primers specific to post oak from reduced genomic libraries. •
  
  
  RESULTS
  Two Q. stellata individuals, sampled from the northern and southern range of the post oak forest, were subject to genomic reduction and 454 pyrosequencing. Bioinformatic analysis identified putative microsatellites from which 12 polymorphic primer sets were screened on three populations. The number of alleles observed ranged from five to 20 across all populations, while observed and expected heterozygosity values ranged from 0.05 to 0.833 and 0.236 to 0.893, respectively, within individual populations. •
  
  
  CONCLUSIONS
  We report the development of microsatellite markers, specific to post oak, to aid the study of genetic diversity and population structure of extant forest remnants.
authors.affiliation: Department of Plant and Wildlife Sciences, Brigham Young University, 5144 Life Sciences Building, Provo, Utah 84602 USA.
authors.forename: Warren B
authors.initials: WB
authors.lastname: Chatwin
authors.affiliation: Department of Plant and Wildlife Sciences, Brigham Young University, 5144 Life Sciences Building, Provo, Utah 84602 USA.
authors.forename: Kyrie K
authors.initials: KK
authors.lastname: Carpenter
authors.affiliation: Department of Plant and Wildlife Sciences, Brigham Young University, 5144 Life Sciences Building, Provo, Utah 84602 USA.
authors.forename: Felix R
authors.initials: FR
authors.lastname: Jimenez
authors.affiliation: Department of Plant and Wildlife Sciences, Brigham Young University, 5144 Life Sciences Building, Provo, Utah 84602 USA.
authors.forename: Dave B
authors.initials: DB
authors.lastname: Elzinga
authors.affiliation: Department of Biology, Brigham Young University, 4058 Life Sciences Building, Provo, Utah 84602 USA.
authors.forename: Leigh A
authors.initials: LA
authors.lastname: Johnson
authors.affiliation: Department of Plant and Wildlife Sciences, Brigham Young University, 5144 Life Sciences Building, Provo, Utah 84602 USA.
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Maughan
keywords: 454 pyrosequencing; Cross Timbers; Quercus stellata; old-growth forest; post oak; simple sequence repeat