title: Inhibition of Salmonella enterica biofilm formation using small-molecule adenosine mimetics.
abstract: Biofilms have been widely implicated in chronic infections and environmental persistence of Salmonella enterica, facilitating enhanced colonization of surfaces and increasing the ability of the bacteria to be transmitted to new hosts. Salmonella enterica serovar Typhi biofilm formation on gallstones from humans and mice enhances gallbladder colonization and bacterial shedding, while Salmonella enterica serovar Typhimurium biofilms facilitate long-term persistence in a number of environments important to food, medical, and farming industries. Salmonella regulates expression of many virulence- and biofilm-related processes using kinase-driven pathways. Kinases play pivotal roles in phosphorylation and energy transfer in cellular processes and possess an ATP-binding pocket required for their functions. Many other cellular proteins also require ATP for their activity. Here we test the hypothesis that pharmacological interference with ATP-requiring enzymes utilizing adenosine mimetic compounds would decrease or inhibit bacterial biofilm formation. Through the screening of a 3,000-member ATP mimetic library, we identified a single compound (compound 7955004) capable of significantly reducing biofilm formation by S. Typhimurium and S. Typhi. The compound was not bactericidal or bacteriostatic toward S. Typhimurium or cytotoxic to mammalian cells. An ATP-Sepharose affinity matrix technique was used to discover potential protein-binding targets of the compound and identified GroEL and DeoD. Compound 7955004 was screened against other known biofilm-forming bacterial species and was found to potently inhibit biofilms of Acinetobacter baumannii as well. The identification of a lead compound with biofilm-inhibiting capabilities toward Salmonella provides a potential new avenue of therapeutic intervention against Salmonella biofilm formation, with applicability to biofilms of other bacterial pathogens.
authors.affiliation: Microbial Infection and Immunity, Center for Microbial Interface Biology, The Ohio State University, Columbus, Ohio, USA.
authors.forename: Jacob A
authors.initials: JA
authors.lastname: Koopman
authors.affiliation: Microbial Infection and Immunity, Center for Microbial Interface Biology, The Ohio State University, Columbus, Ohio, USA.
authors.forename: Joanna M
authors.initials: JM
authors.lastname: Marshall
authors.affiliation: Microbial Infection and Immunity, Center for Microbial Interface Biology, The Ohio State University, Columbus, Ohio, USA.
authors.forename: Aditi
authors.initials: A
authors.lastname: Bhatiya
authors.affiliation: Aklilu Lemma Institute of Pathobiology, Addis Ababa University, Addis Ababa, Ethiopia.
authors.forename: Tadesse
authors.initials: T
authors.lastname: Eguale
authors.affiliation: Microbial Infection and Immunity, Center for Microbial Interface Biology, The Ohio State University, Columbus, Ohio, USA Department of Microbiology, The Ohio State University, Columbus, Ohio, USA.
authors.forename: Jesse J
authors.initials: JJ
authors.lastname: Kwiek
authors.affiliation: Microbial Infection and Immunity, Center for Microbial Interface Biology, The Ohio State University, Columbus, Ohio, USA gunn.43@osu.edu.
authors.forename: John S
authors.initials: JS
authors.lastname: Gunn
mesh_terms: D040981:Acinetobacter baumannii / Q000187:drug effects / Q000254:growth & development / Q000502:physiology*; D000241:Adenosine / Q000031:analogs & derivatives / Q000494:pharmacology*; D000255:Adenosine Triphosphate / Q000737:chemistry; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D018441:Biofilms / Q000187:drug effects / Q000254:growth & development*; D045744:Cell Line, Tumor; D018834:Chaperonin 60 / Q000378:metabolism; D056945:Hep G2 Cells; D006801:Humans; D011485:Protein Binding; D011744:Pyrimidinones / Q000494:pharmacology*; D012485:Salmonella typhi / Q000187:drug effects / Q000254:growth & development / Q000502:physiology*; D012486:Salmonella typhimurium / Q000187:drug effects / Q000254:growth & development / Q000502:physiology*; D013876:Thiophenes / Q000494:pharmacology*
chemical_list: C000600812:7955004 compound; D000900:Anti-Bacterial Agents; D018834:Chaperonin 60; D011744:Pyrimidinones; D013876:Thiophenes; D000255:Adenosine Triphosphate; D000241:Adenosine