title: Changes in subcellular localization of visfatin in human colorectal HCT-116 carcinoma cell line after cytochalasin B treatment.
abstract: The aim of the study was to assess the expression and subcellular localization of visfatin in HCT-116 colorectal carcinoma cells after cytokinesis failure using Cytochalasin B (CytB) and the mechanism of apoptosis of cells after CytB. We observed translocation of visfatin's antigen in cytB treated colorectal carcinoma HCT-116 cells from cytosol to nucleus. Statistical and morphometric analysis revealed significantly higher area-related numerical density visfatin-bound nano-golds in the nuclei of cytB-treated HCT-116 cells compared to cytosol. Reverse relation to visfatin subcellular localization was observed in un-treated HCT-116 cells. The total amount of visfatin protein and visfatin mRNA level in HCT-116 cells was also decreased after CytB treatment. Additionally, CytB significantly decreased cell survival, increased levels of G2/M fractions, induced bi-nuclei formation as well as increased reactive oxygen species (ROS) level in HCT-116 cells. CytB treatment showed cytotoxic effect that stem from oxidative stress and is connected with the changes in the cytoplasmic/nuclear amount of visfatin in HCT-116 cells.
authors.affiliation: Medical University of Silesia. rbuldak@sum.edu.pl.
authors.forename: R J
authors.initials: RJ
authors.lastname: Bułdak
authors.forename: M
authors.initials: M
authors.lastname: Skonieczna
authors.forename: Ł
authors.initials: Ł
authors.lastname: Bułdak
authors.forename: N
authors.initials: N
authors.lastname: Matysiak
authors.forename: Ł
authors.initials: Ł
authors.lastname: Mielańczyk
authors.forename: G
authors.initials: G
authors.lastname: Wyrobiec
authors.forename: M
authors.initials: M
authors.lastname: Kukla
authors.forename: M
authors.initials: M
authors.lastname: Michalski
authors.forename: K
authors.initials: K
authors.lastname: Żwirska-Korczala
mesh_terms: D017209:Apoptosis; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D056148:Chromatography, Reverse-Phase; D015179:Colorectal Neoplasms / Q000378:metabolism*; D003571:Cytochalasin B / Q000494:pharmacology*; D006046:Gold / Q000737:chemistry; D006801:Humans; D049329:Nanostructures / Q000737:chemistry; D054409:Nicotinamide Phosphoribosyltransferase / Q000378:metabolism*; D021381:Protein Transport / Q000187:drug effects
chemical_list: D003571:Cytochalasin B; D006046:Gold; D054409:Nicotinamide Phosphoribosyltransferase