title: Learned stressor resistance requires extracellular signal-regulated kinase in the prefrontal cortex.
abstract: Behaviorally controllable stressors confer protection from the neurochemical and behavioral consequences of future uncontrollable stressors, a phenomenon termed "behavioral immunization". Recent data implicate protein synthesis within the ventromedial prefrontal cortex (mPFC) as critical to behavioral immunization. Adult, male Sprague-Dawley rats were exposed to a series of controllable tailshocks and 1 week later to uncontrollable tailshocks, followed 24 h later by social exploration and shuttlebox escape tests. To test the involvement of N-methyl-D-aspartate receptors (NMDARs) and the extracellular signal-regulated kinase (ERK) cascade in behavioral immunization, either D-AP5 or the MEK inhibitor U0126 was injected to the prelimbic (PL) or infralimbic (IL) mPFC prior to controllable stress exposure. Phosphorylated ERK and P70S6K, regulators of transcription and translation, were quantified by Western blot or immunohistochemistry after controllable or uncontrollable tailshocks. Prior controllable stress prevented the social exploration and shuttlebox performance deficits caused by the later uncontrollable stressor, and this effect was blocked by injections of D-AP5 into mPFC. A significant increase in phosphorylated ERK1 and ERK2, but not P70S6K, occurred within the PL and IL in rats exposed to controllable stress, but not to uncontrollable stress. However, U0126 only prevented behavioral immunization when injected to the PL. We provide evidence that NMDAR and ERK dependent signaling within the PL region is required for behavioral immunization, a learned form of stressor resistance.
authors.affiliation: Center for Neuroscience, Department of Psychology and Neuroscience, University of Colorado Boulder, CO, USA ; Department of Psychology, Boston College Chestnut Hill, MA, USA.
authors.forename: John P
authors.initials: JP
authors.lastname: Christianson
authors.affiliation: Center for Neuroscience, Department of Psychology and Neuroscience, University of Colorado Boulder, CO, USA.
authors.forename: Johanna G
authors.initials: JG
authors.lastname: Flyer-Adams
authors.affiliation: Department of Psychology, University of New Hampshire Durham, NH, USA.
authors.forename: Robert C
authors.initials: RC
authors.lastname: Drugan
authors.affiliation: Center for Neuroscience, Department of Psychology and Neuroscience, University of Colorado Boulder, CO, USA.
authors.forename: Jose
authors.initials: J
authors.lastname: Amat
authors.affiliation: Center for Neuroscience, Department of Psychology and Neuroscience, University of Colorado Boulder, CO, USA.
authors.forename: Rachel A
authors.initials: RA
authors.lastname: Daut
authors.affiliation: Department of Psychology, Boston College Chestnut Hill, MA, USA.
authors.forename: Allison R
authors.initials: AR
authors.lastname: Foilb
authors.affiliation: Center for Neuroscience, Department of Psychology and Neuroscience, University of Colorado Boulder, CO, USA.
authors.forename: Linda R
authors.initials: LR
authors.lastname: Watkins
authors.affiliation: Center for Neuroscience, Department of Psychology and Neuroscience, University of Colorado Boulder, CO, USA.
authors.forename: Steven F
authors.initials: SF
authors.lastname: Maier
keywords: ERK; learned helplessness; plasticity; prefrontal cortex; rat; resilience