title: Microarray multiplex assay for the simultaneous detection and discrimination of influenza a and influenza B viruses.
abstract: In this study, we present a microarray approach for the typing of influenza A and B viruses, and the subtyping of H1 and H3 subtypes. We designed four pairs of specific multiplex RT-PCR primers and eight specific oligonucleotide probes and prepared microarrays to identify the specific subtype of influenza virus. Through amplification and fluorescent marking of the multiplex RT-PCR products on the M gene of influenza A and B viruses and the HA gene of subtypes H1 and H3, the PCR products were hybridized with the microarray, and the results were analyzed using a microarray scanner. The results demonstrate that the chip developed by our research institute can detect influenza A and B viruses specifically and identify the subtypes H1 and H3 at a minimum concentration of 1 × 10(2) copies/μL of viral RNA. We tested 35 clinical samples and our results were identical to other fluorescent methods. The microarray approach developed in this study provides a reliable method for the monitoring and testing of seasonal influenza.
authors.affiliation: College of Animal Science and Veterinary Medicine, Jilin University, Changchun, 130062 People's Republic of China ; Institute of Military Veterinary, Academy of Military Medical Sciences of PLA, Liuying West Road 666, Changchun, 130122 People's Republic of China.
authors.forename: Mingyao
authors.initials: M
authors.lastname: Tian
authors.affiliation: College of Animal Science and Veterinary Medicine, Jilin University, Changchun, 130062 People's Republic of China ; Institute of Military Veterinary, Academy of Military Medical Sciences of PLA, Liuying West Road 666, Changchun, 130122 People's Republic of China.
authors.forename: Yufei
authors.initials: Y
authors.lastname: Tian
authors.affiliation: Department of Respiration, The First Hospital of Jilin University, Changchun, 130021 People's Republic of China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Li
authors.affiliation: Institute of Military Veterinary, Academy of Military Medical Sciences of PLA, Liuying West Road 666, Changchun, 130122 People's Republic of China.
authors.forename: Huijun
authors.initials: H
authors.lastname: Lu
authors.affiliation: Institute of Military Veterinary, Academy of Military Medical Sciences of PLA, Liuying West Road 666, Changchun, 130122 People's Republic of China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Li
authors.affiliation: Institute of Military Veterinary, Academy of Military Medical Sciences of PLA, Liuying West Road 666, Changchun, 130122 People's Republic of China.
authors.forename: Chang
authors.initials: C
authors.lastname: Li
authors.affiliation: Institute of Military Veterinary, Academy of Military Medical Sciences of PLA, Liuying West Road 666, Changchun, 130122 People's Republic of China.
authors.forename: Fei
authors.initials: F
authors.lastname: Xue
authors.affiliation: Institute of Military Veterinary, Academy of Military Medical Sciences of PLA, Liuying West Road 666, Changchun, 130122 People's Republic of China.
authors.forename: Ningyi
authors.initials: N
authors.lastname: Jin
keywords: DNA microarray; Influenza A virus; Influenza B virus; Typing