title: Facile synthesis of folate-conjugated magnetic/fluorescent bifunctional microspheres.
abstract: In this paper, we investigated the functional imaging properties of magnetic microspheres composed of magnetic core and CdTe quantum dots in the silica shell functionalized with folic acid (FA). The preparation procedure included the preparation of chitosan-coated Fe3O4 nanoparticles (CS-coated Fe3O4 NPs) prepared by a one-pot solvothermal method, the reaction between carboxylic and amino groups under activation of NHS and EDC in order to obtain the CdTe-CS-coated Fe3O4 NPs, and finally the growth of SiO2 shell vent the photoluminescence (PL) quenching via a Stöber method (Fe3O4-CdTe@SiO2). Moreover, in order to have a specific targeting capacity, the magnetic and fluorescent bifunctional microspheres were synthesized by bonding of SiO2 shell with FA molecules via amide reaction (Fe3O4-CdTe@SiO2-FA). The morphology, size, chemical components, and magnetic property of as-prepared composite nanoparticles were characterized by ultraviolet-visible spectroscopy, fluorescent spectroscopy, Fourier transform infrared spectroscopy (FTIR), X-ray powder diffraction (XRD), scanning transmission electron microscopy (SEM), transmission electron microscopy (TEM), thermogravimetric analysis (TGA), and vibrating sample magnetometer (VSM), respectively. The results show that the magnetic and fluorescent bifunctional microspheres have strong luminescent which will be employed for immuno-labeling and fluorescent imaging of HeLa cells.
authors.affiliation: College of Pharmaceutical and Chemical Engineering, Taizhou University, Jiaojiang 318000, People's Republic of China.
authors.forename: Mao
authors.initials: M
authors.lastname: Shen
authors.affiliation: College of Pharmaceutical and Chemical Engineering, Taizhou University, Jiaojiang 318000, People's Republic of China.
authors.forename: Wenping
authors.initials: W
authors.lastname: Jia
authors.affiliation: College of Pharmaceutical and Chemical Engineering, Taizhou University, Jiaojiang 318000, People's Republic of China.
authors.forename: Caiping
authors.initials: C
authors.lastname: Lin
authors.affiliation: Key Laboratory of Auxiliary Chemistry and Technology for Chemical Industry, Ministry of Education, Shaanxi University of Science and Technology, Xi'an 710021, China.
authors.forename: Guodong
authors.initials: G
authors.lastname: Fan
authors.affiliation: College of Pharmaceutical Sciences Zhejiang University, Hangzhou 310058, China.
authors.forename: Yangmin
authors.initials: Y
authors.lastname: Jin
authors.affiliation: College of Pharmaceutical and Chemical Engineering, Taizhou University, Jiaojiang 318000, People's Republic of China.
authors.forename: Xiaoying
authors.initials: X
authors.lastname: Chen
authors.affiliation: College of Pharmaceutical and Chemical Engineering, Taizhou University, Jiaojiang 318000, People's Republic of China.
authors.forename: Guang
authors.initials: G
authors.lastname: Chen
keywords: Chitosan; Fluorescent; Magnetic nanoparticles; Solvothermal