title: Genotypic Detection of Epstein Barr Virus from Clinically Suspected Viral Retinitis Patients in a Tertiary Eye Care Centre, India.
abstract: OBJECTIVE
  To evaluate the diagnostic value of PCR on aqueous humour for detection and genotyping of Epstein Bar Virus in patients with viral retinitis.
  
  
  METHODS
  70 AH samples were collected from 20 HIV positive patients with clinically suspected viral retinitis and 25 patients with serpignous choroiditis and 25 AH from patients undergoing cataract surgery. PCR was performed to screen HHV-1 to HHV-5, Mtb and Toxoplasma gondii. Genotype prevalence was confirmed by phylogenetic analysis targetig EBV.
  
  
  RESULTS
  EBV was detected in 17 (37.7%) samples. Genotyping to subtype EBV, revealed the circulation of only one subtype (Type 1). PCR results for other infective agents were negative except for the presence of CMV in 5 (11.1%) AH.
  
  
  CONCLUSIONS
  The application of PCR to detect genotypes can be used as an epidemiological tool for clinical management. To our knowledge this is the first report on genotyping of EBV performed on intra ocular samples.
authors.affiliation: a Vision Research Foundation, L&T Microbiology Research Centre , Sankara Nethralaya , Chennai , India.
authors.forename: Madhuravasal Krishnan
authors.initials: MK
authors.lastname: Janani
authors.affiliation: a Vision Research Foundation, L&T Microbiology Research Centre , Sankara Nethralaya , Chennai , India.
authors.forename: Jambulingam
authors.initials: J
authors.lastname: Malathi
authors.affiliation: a Vision Research Foundation, L&T Microbiology Research Centre , Sankara Nethralaya , Chennai , India.
authors.forename: Jyothirmay
authors.initials: J
authors.lastname: Biswas
authors.affiliation: a Vision Research Foundation, L&T Microbiology Research Centre , Sankara Nethralaya , Chennai , India.
authors.forename: Sudharshan
authors.initials: S
authors.lastname: Sridharan
authors.affiliation: a Vision Research Foundation, L&T Microbiology Research Centre , Sankara Nethralaya , Chennai , India.
authors.forename: Hajib Naraharirao
authors.initials: HN
authors.lastname: Madhavan
mesh_terms: D000328:Adult; D001082:Aqueous Humor / Q000821:virology*; D004279:DNA, Viral / Q000032:analysis*; D020031:Epstein-Barr Virus Infections / Q000453:epidemiology / Q000235:genetics* / Q000821:virology; D015828:Eye Infections, Viral / Q000453:epidemiology / Q000235:genetics* / Q000821:virology; D005260:Female; D005838:Genotype; D004854:Herpesvirus 4, Human / Q000235:genetics*; D006801:Humans; D007194:India / Q000453:epidemiology; D008297:Male; D016133:Polymerase Chain Reaction; D012173:Retinitis / Q000453:epidemiology / Q000235:genetics* / Q000821:virology; D012189:Retrospective Studies; D062606:Tertiary Care Centers
chemical_list: D004279:DNA, Viral
keywords: Detection; Epstein-Barr virus; PCR; genotyping; viral retinitis