title: Identification of sulphur volatiles and GC-olfactometry aroma profiling in two fresh tomato cultivars.
abstract: Ten sulphur volatiles were observed in two Florida tomato cultivars ('Tasti-Lee' and 'FL 47') harvested at three maturity stages (breaker, turning, and pink) using gas chromatography with a pulsed flame photometric detector (GC-PFPD). Eight PFPD peaks were identified using retention values from authentic sulphur standards and GC-MS characteristic masses. Seven were quantified using an internal standard combined with external calibration curves. Dimethyl sulphide, dimethyl disulphide, dimethyl trisulphide 2-propylthiazole and 2-s-butylthiazole were newly identified in fresh tomatoes. Principal component analysis of sulphur volatiles indicated that there were appreciable maturity stage differences clustered in separate quadrants. GC-olfactometry (GC-O) identified 50 aroma-active compounds in 'Tasti-Lee', with 10 reported as odorants in fresh tomatoes for the first time. Four sulphur volatiles exhibited aroma activity, including two of the newly-reported fresh tomato sulphur volatiles, 2-s-butylthiazole and dimethyl sulphide. GC-O aroma profiling indicated that the most intense aroma category was earthy-musty, followed by fruity-floral, green-grassy, sweet-candy and sweaty-stale-sulphurous.
authors.affiliation: Citrus Research and Education Centre, University of Florida, 700 Experiment Station Road, Lake Alfred, FL 33850, USA. Electronic address: xiaofendu09@gmail.com.
authors.forename: Xiaofen
authors.initials: X
authors.lastname: Du
authors.affiliation: Citrus Research and Education Centre, University of Florida, 700 Experiment Station Road, Lake Alfred, FL 33850, USA.
authors.forename: Mei
authors.initials: M
authors.lastname: Song
authors.affiliation: Citrus and Other Subtropical Products, Horticultural Research Laboratory, USDA-ARS, Ft. Pierce, FL 33881, USA.
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Baldwin
authors.affiliation: Citrus Research and Education Centre, University of Florida, 700 Experiment Station Road, Lake Alfred, FL 33850, USA.
authors.forename: Russell
authors.initials: R
authors.lastname: Rouseff
mesh_terms: D002849:Chromatography, Gas / Q000379:methods*; D005638:Fruit / Q000737:chemistry* / Q000254:growth & development; D008401:Gas Chromatography-Mass Spectrometry; D018551:Solanum lycopersicum / Q000737:chemistry*; D009812:Odorants / Q000032:analysis*; D064367:Olfactometry; D012903:Smell; D013457:Sulfur Compounds / Q000032:analysis*; D055549:Volatile Organic Compounds / Q000032:analysis
chemical_list: D013457:Sulfur Compounds; D055549:Volatile Organic Compounds
keywords: FL 47; Tasti-Lee; Tomato GC-O; Tomato sulphur