title: Pharmacokinetic comparisons of benzoylmesaconine in rats using ultra-performance liquid chromatography-tandem mass spectrometry after administration of pure benzoylmesaconine and Wutou decoction.
abstract: Wutou decoction is widely used in China because of its therapeutic effect on rheumatoid arthritis. Benzoylmesaconine (BMA), the most abundant component of Wutou decoction, was used as the marker compound for the pharmacokinetic study of Wutou decoction. The aim of the present study was to compare the pharmacokinetics of BMA in rats after oral administration of pure BMA and Wutou decoction. Pure BMA (5 mg/kg) and Wutou decoction (0.54 g/kg, equivalent to 5 mg/kg BMA) were orally administered to rats with blood samples collected over 10 h. Quantiﬁcation of BMA in rat plasma was achieved using sensitive and validated ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). Specifically, the half-life (T1/2) and mean residence time values of pure BMA were 228.3 ± 117.0 min and 155.0 ± 33.2 min, respectively, whereas those of BMA in Wutou decoction were decreased to 61.8 ± 35.1 min and 55.8 ± 16.4 min, respectively. The area under the curve (AUC) of BMA after administration of Wutou decoction was significantly decreased (five-fold) compared with that of pure BMA. The results indicate that the elimination of BMA in rats after the administration of Wutou decoction was significantly faster compared with that of pure BMA.
authors.affiliation: Department of Pharmaceutics, School of Pharmaceutical Sciences, Southern Medical University, Guangzhou 510515, Guangdong, China. daipeimin1989@163.com.
authors.forename: Pei-Min
authors.initials: PM
authors.lastname: Dai
authors.affiliation: Department of Pharmaceutics, School of Pharmaceutical Sciences, Southern Medical University, Guangzhou 510515, Guangdong, China. IITCM_Wangy@126.com.
authors.forename: Ying
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Pharmaceutics, School of Pharmaceutical Sciences, Southern Medical University, Guangzhou 510515, Guangdong, China. lotus623@126.com.
authors.forename: Ling
authors.initials: L
authors.lastname: Ye
authors.affiliation: Department of Pharmaceutics, School of Pharmaceutical Sciences, Southern Medical University, Guangzhou 510515, Guangdong, China. zengshan_jessica@126.com.
authors.forename: Shan
authors.initials: S
authors.lastname: Zeng
authors.affiliation: Department of Pharmaceutics, School of Pharmaceutical Sciences, Southern Medical University, Guangzhou 510515, Guangdong, China. zhijiezheng@126.com.
authors.forename: Zhi-Jie
authors.initials: ZJ
authors.lastname: Zheng
authors.affiliation: Department of Pharmaceutics, School of Pharmaceutical Sciences, Southern Medical University, Guangzhou 510515, Guangdong, China. liqiangsmu@163.com.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Li
authors.affiliation: Department of Pharmaceutics, School of Pharmaceutical Sciences, Southern Medical University, Guangzhou 510515, Guangdong, China. iitcm_lu@126.com.
authors.forename: Lin-Liu
authors.initials: LL
authors.lastname: Lu
authors.affiliation: Department of Pharmaceutics, School of Pharmaceutical Sciences, Southern Medical University, Guangzhou 510515, Guangdong, China. liuzq@gzucm.edu.cn.
authors.forename: Zhong-Qiu
authors.initials: ZQ
authors.lastname: Liu
mesh_terms: D000157:Aconitine / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000493:pharmacokinetics / Q000494:pharmacology; D031841:Aconitum / Q000737:chemistry*; D000284:Administration, Oral; D000818:Animals; D019540:Area Under Curve; D002681:China; D002853:Chromatography, Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000493:pharmacokinetics* / Q000494:pharmacology; D006207:Half-Life; D008297:Male; D008958:Models, Molecular; D062105:Molecular Docking Simulation; D015394:Molecular Structure; D010936:Plant Extracts / Q000008:administration & dosage / Q000493:pharmacokinetics* / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D053719:Tandem Mass Spectrometry / Q000379:methods*; D014018:Tissue Distribution; D032413:Viola / Q000737:chemistry*
chemical_list: D004365:Drugs, Chinese Herbal; D010936:Plant Extracts; C085283:benzoylmesaconine; D000157:Aconitine