title: Proteomic analysis of human fetal atria and ventricle.
abstract: In this study we carried out a mass spectrometry-based proteome analysis of human fetal atria and ventricles. Heart protein lysates were analyzed on the Q-Exactive mass spectrometer in biological triplicates. Protein identification using MaxQuant yielded a total of 2754 atrial protein groups (91%) and 2825 ventricular protein groups (83%) in at least 2 of the 3 runs with ≥ 2 unique peptides. Statistical analyses using fold-enrichment (>2) and p-values (≤ 0.05) selected chamber-enriched atrial (134) and ventricular (81) protein groups. Several previously characterized cardiac chamber-enriched proteins were identified in this study including atrial isoform of myosin light chain 2 (MYL7), atrial natriuretic peptide (NPPA), connexin 40 (GJA5), and peptidylglycine alpha-amidating monooxygenase (PAM) for atria, and ventricular isoforms of myosin light chains (MYL2 and MYL3), myosin heavy chain 7 (MYH7), and connexin 43 (GJA1) for ventricle. Our data was compared to in-house generated and publicly available human microarrays, several human cardiac proteomes, and phenotype ontology databases.
authors.affiliation: Department of Physiology, ‡Medical Biophysics, Faculty of Medicine, and §Heart and Stroke/Richard Lewar Centre of Excellent for Cardiovascular Research, University of Toronto , Toronto, Ontario, Canada.
authors.forename: Zhen Qi
authors.initials: ZQ
authors.lastname: Lu
authors.forename: Ankit
authors.initials: A
authors.lastname: Sinha
authors.forename: Parveen
authors.initials: P
authors.lastname: Sharma
authors.forename: Thomas
authors.initials: T
authors.lastname: Kislinger
authors.forename: Anthony O
authors.initials: AO
authors.lastname: Gramolini
mesh_terms: D005333:Fetus / Q000378:metabolism; D015870:Gene Expression; D006325:Heart Atria / Q000378:metabolism*; D006352:Heart Ventricles / Q000378:metabolism*; D006801:Humans; D009124:Muscle Proteins / Q000235:genetics / Q000378:metabolism*; D009928:Organ Specificity; D020543:Proteome / Q000235:genetics / Q000378:metabolism*; D040901:Proteomics
chemical_list: D009124:Muscle Proteins; D020543:Proteome
keywords: Bioinformatics; Q-exactive; chamber specificity; fetal tissue; mass spectrometry; ventricle