title: Acute human parvovirus b19 infection: cytologic diagnosis.
abstract: Human parvovirus B19 is highly tropic to human bone marrow and replicates only in erythroid progenitor cells. It is causative agent of transient aplastic crisis in patients with chronic haemolytic anemia. In immunocompromised patients persistent parvovirus B19 infection may develop and it manifests as pure red cell aplasia and chronic anaemia. Bone marrow is characterised morphologically by giant pronormoblast stage with little or no further maturation. We encountered a case of 6 year old HIV positive male child presented with pure red cell aplasia due to parvovirus B19 infection. Bone marrow aspiration cytology revealed giant pronormoblast with prominent intranuclear inclusions led to suspicion of parvovirus B19 infection which was confirmed by DNA PCR. This case is presented to report classical morphological features of parvovirus B19 infection rarely seen on bone marrow examination should warrant the suspicion of human parvovirus B19 infection in the setting of HIV positive patient with repeated transfusions and confirmation should be done by PCR.
authors.affiliation: Department of Pathology, Byramjee Jeejeebhoy Government Medical College, A-7, Devi chambers, 23/5 A, Bund Garden Road, Pune, 411001 Maharashtra India.
authors.forename: Rane
authors.initials: R
authors.lastname: Sharada Raju
authors.affiliation: Department of Pathology, Byramjee Jeejeebhoy Government Medical College, B/403, Nancy Lake Homes, Opposite Bharati Vidyapeeth, Pune-Satara Road, Katraj, Pune, 411046 Maharashtra India.
authors.forename: Kadgi
authors.initials: K
authors.lastname: Nalini Vinayak
authors.affiliation: Department of Pathology, Private Consultant Histopathologist, Vidish, Opposite Kamala Nehru Park, Erandwane, Pune, Maharashtra India.
authors.forename: Vishnuprasad
authors.initials: V
authors.lastname: Madhusudan Bapat
authors.affiliation: Department of Pathology, Byramjee Jeejeebhoy Government Medical College, Pune, Maharashtra India ; 174 A, Road No P, Bhupalpura, Udaipur, 313001 Rajasthan India.
authors.forename: Agrawal
authors.initials: A
authors.lastname: Preeti Balkisanji
authors.affiliation: Department of Pathology, Byramjee Jeejeebhoy Government Medical College, Pune, Maharashtra India.
authors.forename: Puranik
authors.initials: P
authors.lastname: Shaila Chandrakant
keywords: HIV; Parvovirus; Pure red cell aplasia