title: Genetic approaches in the study of heparan sulfate functions in Drosophila.
abstract: Several classes of heparan sulfate proteoglycan (HSPG) core proteins and all HS biosynthetic/modifying enzymes are evolutionarily conserved from human to Drosophila melanogaster. This genetically tractable model offers highly sophisticated techniques to manipulate gene function in a spatially and temporally controlled manner. Thus, Drosophila has been a powerful system to explore the functions of HSPGs in vivo. In this chapter, we will introduce two genetic techniques available in Drosophila: TARGET (temporal and regional gene expression targeting) and MARCM (mosaic analysis with a repressible cell marker).
authors.affiliation: Department of Genetics, Cell Biology and Development, University of Minnesota, Minneapolis, MN, 55455, USA.
authors.forename: Masahiko
authors.initials: M
authors.lastname: Takemura
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Nakato
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D017124:Conserved Sequence; D004331:Drosophila melanogaster / Q000166:cytology / Q000235:genetics*; D019143:Evolution, Molecular; D005821:Genetic Techniques; D006497:Heparan Sulfate / Q000378:metabolism*; D006801:Humans; D009030:Mosaicism; D013429:Sulfatases / Q000378:metabolism
chemical_list: D006497:Heparan Sulfate; D013429:Sulfatases