title: Development of EST-SSR markers and construction of a linkage map in faba bean (Vicia faba).
abstract: To develop a high density linkage map in faba bean, a total of 1,363 FBES (Faba bean expressed sequence tag [EST]-derived simple sequence repeat [SSR]) markers were designed based on 5,090 non-redundant ESTs developed in this study. A total of 109 plants of a 'Nubaria 2' × 'Misr 3' F2 mapping population were used for map construction. Because the parents were not pure homozygous lines, the 109 F2 plants were divided into three subpopulations according to the original F1 plants. Linkage groups (LGs) generated in each subpopulation were integrated by commonly mapped markers. The integrated 'Nubaria 2' × 'Misr 3' map consisted of six LGs, representing a total length of 684.7 cM, with 552 loci. Of the mapped loci, 47% were generated from multi-loci diagnostic (MLD) markers. Alignment of homologous sequence pairs along each linkage group revealed obvious syntenic relationships between LGs in faba bean and the genomes of two model legumes, Lotus japonicus and Medicago truncatula. In a polymorphic analysis with ten Egyptian faba bean varieties, 78.9% (384/487) of the FBES markers showed polymorphisms. Along with the EST-SSR markers, the dense map developed in this study is expected to accelerate marker assisted breeding in faba bean.
authors.affiliation: Sakha Agricultural Research Station, Field Crop Research Institute, Agricultural Research Center , P.O. Box 33717, Kafr Sl-Sheikh , Egypt.
authors.forename: Walid
authors.initials: W
authors.lastname: El-Rodeny
authors.affiliation: Kazusa DNA Research Institute , 2-6-7 Kazusa-Kamatari, Kisarazu, Chiba 292-0818 , Japan.
authors.forename: Mitsuhiro
authors.initials: M
authors.lastname: Kimura
authors.affiliation: Kazusa DNA Research Institute , 2-6-7 Kazusa-Kamatari, Kisarazu, Chiba 292-0818 , Japan.
authors.forename: Hideki
authors.initials: H
authors.lastname: Hirakawa
authors.affiliation: Sakha Agricultural Research Station, Field Crop Research Institute, Agricultural Research Center , P.O. Box 33717, Kafr Sl-Sheikh , Egypt.
authors.forename: Attia
authors.initials: A
authors.lastname: Sabah
authors.affiliation: Kazusa DNA Research Institute , 2-6-7 Kazusa-Kamatari, Kisarazu, Chiba 292-0818 , Japan.
authors.forename: Kenta
authors.initials: K
authors.lastname: Shirasawa
authors.affiliation: Kazusa DNA Research Institute , 2-6-7 Kazusa-Kamatari, Kisarazu, Chiba 292-0818 , Japan.
authors.forename: Shusei
authors.initials: S
authors.lastname: Sato
authors.affiliation: Kazusa DNA Research Institute , 2-6-7 Kazusa-Kamatari, Kisarazu, Chiba 292-0818 , Japan.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Tabata
authors.affiliation: Kazusa DNA Research Institute , 2-6-7 Kazusa-Kamatari, Kisarazu, Chiba 292-0818 , Japan.
authors.forename: Shigemi
authors.initials: S
authors.lastname: Sasamoto
authors.affiliation: Kazusa DNA Research Institute , 2-6-7 Kazusa-Kamatari, Kisarazu, Chiba 292-0818 , Japan.
authors.forename: Akiko
authors.initials: A
authors.lastname: Watanabe
authors.affiliation: Kazusa DNA Research Institute , 2-6-7 Kazusa-Kamatari, Kisarazu, Chiba 292-0818 , Japan.
authors.forename: Kumiko
authors.initials: K
authors.lastname: Kawashima
authors.affiliation: Kazusa DNA Research Institute , 2-6-7 Kazusa-Kamatari, Kisarazu, Chiba 292-0818 , Japan.
authors.forename: Midori
authors.initials: M
authors.lastname: Kato
authors.affiliation: Kazusa DNA Research Institute , 2-6-7 Kazusa-Kamatari, Kisarazu, Chiba 292-0818 , Japan.
authors.forename: Tsuyuko
authors.initials: T
authors.lastname: Wada
authors.affiliation: Kazusa DNA Research Institute , 2-6-7 Kazusa-Kamatari, Kisarazu, Chiba 292-0818 , Japan.
authors.forename: Hisano
authors.initials: H
authors.lastname: Tsuruoka
authors.affiliation: Kazusa DNA Research Institute , 2-6-7 Kazusa-Kamatari, Kisarazu, Chiba 292-0818 , Japan.
authors.forename: Chika
authors.initials: C
authors.lastname: Takahashi
authors.affiliation: Kazusa DNA Research Institute , 2-6-7 Kazusa-Kamatari, Kisarazu, Chiba 292-0818 , Japan.
authors.forename: Chiharu
authors.initials: C
authors.lastname: Minami
authors.affiliation: Kazusa DNA Research Institute , 2-6-7 Kazusa-Kamatari, Kisarazu, Chiba 292-0818 , Japan.
authors.forename: Keiko
authors.initials: K
authors.lastname: Nanri
authors.affiliation: Kazusa DNA Research Institute , 2-6-7 Kazusa-Kamatari, Kisarazu, Chiba 292-0818 , Japan.
authors.forename: Shinobu
authors.initials: S
authors.lastname: Nakayama
authors.affiliation: Kazusa DNA Research Institute , 2-6-7 Kazusa-Kamatari, Kisarazu, Chiba 292-0818 , Japan.
authors.forename: Mitsuyo
authors.initials: M
authors.lastname: Kohara
authors.affiliation: Kazusa DNA Research Institute , 2-6-7 Kazusa-Kamatari, Kisarazu, Chiba 292-0818 , Japan.
authors.forename: Manabu
authors.initials: M
authors.lastname: Yamada
authors.affiliation: Kazusa DNA Research Institute , 2-6-7 Kazusa-Kamatari, Kisarazu, Chiba 292-0818 , Japan.
authors.forename: Yoshie
authors.initials: Y
authors.lastname: Kishida
authors.affiliation: Kazusa DNA Research Institute , 2-6-7 Kazusa-Kamatari, Kisarazu, Chiba 292-0818 , Japan.
authors.forename: Tsunakazu
authors.initials: T
authors.lastname: Fujishiro
authors.affiliation: Kazusa DNA Research Institute , 2-6-7 Kazusa-Kamatari, Kisarazu, Chiba 292-0818 , Japan.
authors.forename: Sachiko
authors.initials: S
authors.lastname: Isobe
keywords: EST-SSR; comparative mapping; faba bean; genetic diversity; linkage map