title: Design strategies for bioorthogonal smart probes.
abstract: Bioorthogonal chemistry has enabled the selective labeling and detection of biomolecules in living systems. Bioorthogonal smart probes, which become fluorescent or deliver imaging or therapeutic agents upon reaction, allow for the visualization of biomolecules or targeted delivery even in the presence of excess unreacted probe. This review discusses the strategies used in the development of bioorthogonal smart probes and highlights the potential of these probes to further our understanding of biology.
authors.affiliation: Department of Chemistry, University of California, Berkeley, California 94720, USA.
authors.forename: Peyton
authors.initials: P
authors.lastname: Shieh
authors.forename: Carolyn R
authors.initials: CR
authors.lastname: Bertozzi
mesh_terms: D000447:Aldehydes / Q000737:chemistry; D000480:Alkynes / Q000737:chemistry; D001386:Azides / Q000737:chemistry; D057930:Click Chemistry; D061565:Cycloaddition Reaction; D004337:Drug Carriers / Q000138:chemical synthesis* / Q000737:chemistry; D031541:Fluorescence Resonance Energy Transfer; D005456:Fluorescent Dyes / Q000138:chemical synthesis* / Q000737:chemistry; D006801:Humans; D057054:Molecular Imaging; D015335:Molecular Probes / Q000138:chemical synthesis* / Q000737:chemistry; D010084:Oxidation-Reduction; D010091:Oximes / Q000737:chemistry; D010720:Phosphines / Q000737:chemistry; D013194:Staining and Labeling / Q000379:methods*
chemical_list: D000447:Aldehydes; D000480:Alkynes; D001386:Azides; D004337:Drug Carriers; D005456:Fluorescent Dyes; D015335:Molecular Probes; D010091:Oximes; D010720:Phosphines