title: A novel PCR-based approach to discover miRNA target genes.
abstract: MiRNAs are potent regulators of gene expression, and most miRNAs have from several to several thousands of gene targets. Validating the numerous gene targets of a given miRNA remains challenging despite the existence of various tools and databases that predict candidate gene-miRNA pairs. In the present study, we present a high-throughput but flexible method that applies a PCR-based application to simulate the binding of miRNAs to their gene targets. Using hsa-miR-377 as an illustrative example, our method was able to identify 13 potential targets of hsa-miR-377. Moreover, our results include 2 genes (SOD2 and PPM1A) that have already been verified as targets of hsa-miR-377. Our method may provide an alternative way of identifying the gene targets of miRNAs for future research.
authors.affiliation: 1. Zhejiang Provincial Key Laboratory of Pathophysiology, School of Medicine, Ningbo University, Ningbo, ZJ 315000, China.
authors.forename: Shiwei
authors.initials: S
authors.lastname: Duan
authors.affiliation: 2. The Neurology Department of the 148th Hospital of PLA, Zibo, SD 255300, China.
authors.forename: Yunliang
authors.initials: Y
authors.lastname: Wang
authors.affiliation: 3. Department of Medicine, University of Chicago, Chicago, IL 60637, USA.
authors.forename: Hongwei
authors.initials: H
authors.lastname: Wang
authors.affiliation: 4. Biological Science Division, University of Chicago, Chicago, IL 60637, USA.
authors.forename: Shufei
authors.initials: S
authors.lastname: Wang
authors.affiliation: 1. Zhejiang Provincial Key Laboratory of Pathophysiology, School of Medicine, Ningbo University, Ningbo, ZJ 315000, China.
authors.forename: Lindan
authors.initials: L
authors.lastname: Ji
authors.affiliation: 1. Zhejiang Provincial Key Laboratory of Pathophysiology, School of Medicine, Ningbo University, Ningbo, ZJ 315000, China.
authors.forename: Dongjun
authors.initials: D
authors.lastname: Dai
authors.affiliation: 1. Zhejiang Provincial Key Laboratory of Pathophysiology, School of Medicine, Ningbo University, Ningbo, ZJ 315000, China.
authors.forename: Danjie
authors.initials: D
authors.lastname: Jiang
authors.affiliation: 2. The Neurology Department of the 148th Hospital of PLA, Zibo, SD 255300, China.
authors.forename: Xiaoxi
authors.initials: X
authors.lastname: Zhang
authors.affiliation: 5. Department of Psychiatry, University of Chicago, Chicago, IL 60637, USA.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Wang
mesh_terms: D020413:3' Untranslated Regions; D001665:Binding Sites / Q000235:genetics; D002478:Cells, Cultured; D005786:Gene Expression Regulation; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods; D006801:Humans; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D010749:Phosphoprotein Phosphatases / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods*; D000071636:Protein Phosphatase 2C; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013482:Superoxide Dismutase / Q000235:genetics; D000099156:Superoxide Dismutase 2
chemical_list: D020413:3' Untranslated Regions; D035683:MicroRNAs; D010749:Phosphoprotein Phosphatases; D000071636:Protein Phosphatase 2C; D012333:RNA, Messenger; D013482:Superoxide Dismutase; D000099156:Superoxide Dismutase 2; C534063:MIRN377 microRNA, human; C000607456:PPM1A protein, human
keywords: gene target; high-throughput.; hsa-miR-377; miRNA