title: Effect of postthaw storage time and sperm-to-egg ratio on fertility of cryopreserved brook trout sperm.
abstract: The aim of this study was to test the influence of postthaw storage time on sperm motility parameters of brook trout (n = 9). Furthermore, we examined the effect of sperm-to-egg ratios of 300,000:1 and 600,000:1 on fertility of postthaw, cryopreserved, brook trout sperm. The application of a cryopreservation procedure produced very high postthaw sperm motility (56.8 ± 4.0%). The cryopreserved sperm of brook trout could be stored up to 60 minutes without loss of the percentage of sperm motility (52.0 ± 9.0%). The fertilization capacity of brook trout postthaw sperm was comparable with the fertilization rate of fresh semen at a sperm-to-egg ratio as low as 300,000:1 (42.4 ± 14.0% and 36.5 ± 11.0% for eyed and hatched stages, respectively). The possibility of postthaw semen storage for the prolonged time and the obtainment of high fertilization rate at low sperm-to-egg ratio can lead to the significant improvement of brook trout semen cryopreservation procedure.
authors.affiliation: Department of Gametes and Embryo Biology, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Olsztyn, Poland. Electronic address: j.nynca@pan.olsztyn.pl.
authors.forename: J
authors.initials: J
authors.lastname: Nynca
authors.affiliation: Department of Gametes and Embryo Biology, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Olsztyn, Poland.
authors.forename: G J
authors.initials: GJ
authors.lastname: Dietrich
authors.affiliation: Department of Salmonid Research, Inland Fisheries Institute, Żukowo, Poland.
authors.forename: S
authors.initials: S
authors.lastname: Dobosz
authors.affiliation: Department of Salmonid Research, Inland Fisheries Institute, Żukowo, Poland.
authors.forename: T
authors.initials: T
authors.lastname: Zalewski
authors.affiliation: Department of Gametes and Embryo Biology, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Olsztyn, Poland.
authors.forename: A
authors.initials: A
authors.lastname: Ciereszko
mesh_terms: D000818:Animals; D002452:Cell Count; D015925:Cryopreservation / Q000662:veterinary*; D005260:Female; D005298:Fertility; D006358:Hot Temperature; D008297:Male; D010063:Ovum; D012662:Semen Preservation / Q000379:methods / Q000662:veterinary*; D013081:Sperm Motility; D013084:Sperm-Ovum Interactions; D013094:Spermatozoa / Q000502:physiology*; D013997:Time Factors; D014337:Trout
keywords: Computer-assisted sperm analysis; Cryopreservation; Fertilizing ability; Fish; Milt