title: Lack of association between UBE2E2 gene polymorphism (rs7612463) and type 2 diabetes mellitus in a Saudi population.
abstract: The ubiquitin-conjugating enzyme E2E 2 (UBE2E2) gene plays an important role in insulin synthe-sis and secretion under conditions in which stress to the endoplasmic reticu-lum is increased in β-cells. In this case-control study, we have selected rs7612462 polymorphism within UBE2E2 gene to identify in a Saudi population the type 2 diabetes mellitus (T2DM) subjects. In total, 376 subjects with T2DM and 380 controls were enrolled in this study. We have collected 5 mL of peripheral blood from each participant for biochemical and molecular analyses. PCR-RFLP was used to generate genotypes at rs7612462 in all of the study subjects. Clinical data and anthropometric measurements of the patients were significantly different from those of the controls (p<0.05). All of the subjects used in this study were non-obese (25<BMI<30). None of the alleles or genotypes of rs7612462 were associated with T2DM (OR=1.251, 95% CI=0.7703-2.034; p=0.3641). Our data suggest that rs7612462 polymorphism in UBE2E2 does not contribute to T2DM susceptibility in the Saudi population.
authors.affiliation: Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Saud University, Riyadh, Kingdom of Saudi Arabia.
authors.forename: Khalid Khalaf
authors.initials: KK
authors.lastname: Alharbi
authors.affiliation: Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Saud University, Riyadh, Kingdom of Saudi Arabia.
authors.forename: Imran Ali
authors.initials: IA
authors.lastname: Khan
authors.affiliation: Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Saud University, Riyadh, Kingdom of Saudi Arabia.
authors.forename: Yazeed A
authors.initials: YA
authors.lastname: Al-Sheikh
authors.affiliation: Department of Biology Science, College of Science and Arts, Al-Qassim University, Buraidah, Kingdom of Saudi Arabia.
authors.forename: Fawiziah Khalaf
authors.initials: FK
authors.lastname: Alharbi
authors.affiliation: Saudi Programme of Internal Medicine, King Fahad Specialist Hospital-Al-Qassim, Kingdom of Saudi Arabia.
authors.forename: Fahad Khalaf
authors.initials: FK
authors.lastname: Alharbi
authors.affiliation: Stem Cell Units, Anatomy Department, College of Medicine, King Khalid University Hospital and Preparatory Year-Saudi Electronic University, Riyadh, Kingdom of Saudi Arabia.
authors.forename: May Salem
authors.initials: MS
authors.lastname: Al-Nbaheen
mesh_terms: D000328:Adult; D003924:Diabetes Mellitus, Type 2 / Q000235:genetics*; D005260:Female; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic / Q000235:genetics*; D012529:Saudi Arabia; D044763:Ubiquitin-Conjugating Enzymes / Q000235:genetics*
chemical_list: C106347:UBE2E2 protein, human; D044763:Ubiquitin-Conjugating Enzymes