title: Characterization of N-Acyl-homoserine Lactones (AHLs)-Deficient Clinical Isolates of Pseudomonas aeruginosa.
abstract: Pseudomonas aeruginosa is an opportunistic pathogen causing severe respiratory infections. Acylated homoserine lactones (AHLs) are self-generated diffusible signal molecules that mediate population density dependent gene expression (quorum sensing, QS) in a variety of Gram-negative bacteria, and several virulence genes of bacterial pathogens are known to be controlled by QS. Hence, fitness mutant of virulent factors is beneficial for natural selection. In this study, strains of P. aeruginosa isolated from chronic lung infection of cystic fibrosis patients, were screened for AHLs production by using indicator strains of Chromobacterium violaceum CV026 and Agrobacterium tumefaciens strain At136. Four AHLs defective strains were selected from fifty-three clinical isolates. PCR analysis revealed that only one isolate was negative for lasR gene. These four AHLs defective isolates produced less virulence factors and forming less biofilm than PAO1. Only isolate PA41 produce little more pyocyanin than PAO1. The results indicate that, despite the pivotal role of QS in the pathogenesis of P. aeruginosa infections, AHLs-deficient strains are still capable of causing infections in human.
authors.affiliation: Lishui People's Hospital, Lishui, 323000 People's Republic of China.
authors.forename: Zhenqiu
authors.initials: Z
authors.lastname: Shang
authors.affiliation: Lishui People's Hospital, Lishui, 323000 People's Republic of China.
authors.forename: Huafu
authors.initials: H
authors.lastname: Wang
authors.affiliation: School of International Pharmaceutical Business, China Pharmaceutical University, Nanjing, 210009 People's Republic of China.
authors.forename: Shuxin
authors.initials: S
authors.lastname: Zhou
authors.affiliation: Department of Microbiology, School of Life Science and Technology, China Pharmaceutical University, Nanjing, 210009 People's Republic of China.
authors.forename: Weihua
authors.initials: W
authors.lastname: Chu
keywords: Acylated homoserine lactones; Biofilm; Deficient; Pseudomonas aeruginosa; Virulence factors