title: Pentachlorophenol degradation by Janibacter sp., a new actinobacterium isolated from saline sediment of arid land.
abstract: Many pentachlorophenol- (PCP-) contaminated environments are characterized by low or elevated temperatures, acidic or alkaline pH, and high salt concentrations. PCP-degrading microorganisms, adapted to grow and prosper in these environments, play an important role in the biological treatment of polluted extreme habitats. A PCP-degrading bacterium was isolated and characterized from arid and saline soil in southern Tunisia and was enriched in mineral salts medium supplemented with PCP as source of carbon and energy. Based on 16S rRNA coding gene sequence analysis, the strain FAS23 was identified as Janibacter sp. As revealed by high performance liquid chromatography (HPLC) analysis, FAS23 strain was found to be efficient for PCP removal in the presence of 1% of glucose. The conditions of growth and PCP removal by FAS23 strain were found to be optimal in neutral pH and at a temperature of 30 °C. Moreover, this strain was found to be halotolerant at a range of 1-10% of NaCl and able to degrade PCP at a concentration up to 300 mg/L, while the addition of nonionic surfactant (Tween 80) enhanced the PCP removal capacity.
authors.affiliation: Université Tunis El Manar, Faculté des Sciences de Tunis (FST), LR03ES03 Laboratoire de Microorganisme et Biomolécules Actives, Campus Universitaire, 2092 Tunis, Tunisia ; Laboratoire de Traitement et Recyclage des Eaux, Centre des Recherches et Technologie des Eaux (CERTE), Technopôle Borj-Cédria, B.P. 273, 8020 Soliman, Tunisia.
authors.forename: Amel
authors.initials: A
authors.lastname: Khessairi
authors.affiliation: Université Tunis El Manar, Faculté des Sciences de Tunis (FST), LR03ES03 Laboratoire de Microorganisme et Biomolécules Actives, Campus Universitaire, 2092 Tunis, Tunisia.
authors.forename: Imene
authors.identifier: 0000-0001-6416-685X
authors.initials: I
authors.lastname: Fhoula
authors.affiliation: Université Tunis El Manar, Faculté des Sciences de Tunis (FST), LR03ES03 Laboratoire de Microorganisme et Biomolécules Actives, Campus Universitaire, 2092 Tunis, Tunisia.
authors.forename: Atef
authors.initials: A
authors.lastname: Jaouani
authors.affiliation: Laboratoire de Traitement et Recyclage des Eaux, Centre des Recherches et Technologie des Eaux (CERTE), Technopôle Borj-Cédria, B.P. 273, 8020 Soliman, Tunisia.
authors.forename: Yousra
authors.initials: Y
authors.lastname: Turki
authors.affiliation: Université de Manouba, Institut Supérieur de Biotechnologie de Sidi Thabet, LR11ES31 Laboratoire de Biotechnologie et Valorization des Bio-Geo Resources, Biotechpole de Sidi Thabet, 2020 Ariana, Tunisia.
authors.forename: Ameur
authors.identifier: 0000-0001-7310-3842
authors.initials: A
authors.lastname: Cherif
authors.affiliation: Université Tunis El Manar, Faculté des Sciences de Tunis (FST), LR03ES03 Laboratoire de Microorganisme et Biomolécules Actives, Campus Universitaire, 2092 Tunis, Tunisia.
authors.forename: Abdellatif
authors.initials: A
authors.lastname: Boudabous
authors.affiliation: Laboratoire de Traitement et Recyclage des Eaux, Centre des Recherches et Technologie des Eaux (CERTE), Technopôle Borj-Cédria, B.P. 273, 8020 Soliman, Tunisia.
authors.forename: Abdennaceur
authors.initials: A
authors.lastname: Hassen
authors.affiliation: Université Tunis El Manar, Faculté des Sciences de Tunis (FST), LR03ES03 Laboratoire de Microorganisme et Biomolécules Actives, Campus Universitaire, 2092 Tunis, Tunisia.
authors.forename: Hadda
authors.initials: H
authors.lastname: Ouzari
mesh_terms: D039903:Actinobacteria / Q000187:drug effects / Q000254:growth & development / Q000302:isolation & purification* / Q000378:metabolism*; D001673:Biodegradation, Environmental / Q000187:drug effects; D003889:Desert Climate; D019015:Geologic Sediments / Q000382:microbiology*; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D010416:Pentachlorophenol / Q000378:metabolism*; D010802:Phylogeny; D011136:Polysorbates / Q000494:pharmacology; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D054712:Salinity; D012965:Sodium Chloride / Q000494:pharmacology; D013501:Surface-Active Agents / Q000494:pharmacology; D013696:Temperature
chemical_list: D011136:Polysorbates; D012336:RNA, Ribosomal, 16S; D013501:Surface-Active Agents; D012965:Sodium Chloride; D010416:Pentachlorophenol