title: Recent trends in the analysis of vitamin D and its metabolites in milk--a review.
abstract: Vitamin D plays an important role in calcium metabolism and affects other metabolic pathways. Despite the intense interest in vitamin D, no comprehensive overview addressing the analysis of vitamin D in milk has been published. Historically, immunoassay techniques have been mainly used for the routine quantification of vitamin D and its metabolites. However, the greater accuracy and precision of chromatography makes it one of the most important methods in the analysis of vitamin D. The determination of vitamin D and its metabolites by LC-MS is the gold standard for its assessment. LC-MS has unique advantages for vitamin D determination and quantification due to its high sensitivity and specificity. In this review, the current status of vitamin D and its metabolites analysis in milk, human and bovine, including sample pre-treatment and chromatography analysis, are critically discussed and summarised.
authors.affiliation: Department of Analytical Chemistry, Faculty of Pharmacy, Charles University, Heyrovského 1203, 500 05 Hradec Králové, Czech Republic; III. Internal Gerontometabolic Clinic, University Hospital, Sokolská 581, 500 05 Hradec Králové, Czech Republic.
authors.forename: Eva
authors.initials: E
authors.lastname: Kasalová
authors.affiliation: Department of Analytical Chemistry, Faculty of Pharmacy, Charles University, Heyrovského 1203, 500 05 Hradec Králové, Czech Republic; III. Internal Gerontometabolic Clinic, University Hospital, Sokolská 581, 500 05 Hradec Králové, Czech Republic.
authors.forename: Jana
authors.initials: J
authors.lastname: Aufartová
authors.affiliation: Department of Analytical Chemistry, Faculty of Pharmacy, Charles University, Heyrovského 1203, 500 05 Hradec Králové, Czech Republic; III. Internal Gerontometabolic Clinic, University Hospital, Sokolská 581, 500 05 Hradec Králové, Czech Republic.
authors.forename: Lenka Kujovská
authors.initials: LK
authors.lastname: Krčmová
authors.affiliation: III. Internal Gerontometabolic Clinic, University Hospital, Sokolská 581, 500 05 Hradec Králové, Czech Republic.
authors.forename: Dagmar
authors.initials: D
authors.lastname: Solichová
authors.affiliation: Department of Analytical Chemistry, Faculty of Pharmacy, Charles University, Heyrovského 1203, 500 05 Hradec Králové, Czech Republic.
authors.forename: Petr
authors.initials: P
authors.lastname: Solich
mesh_terms: D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D002853:Chromatography, Liquid; D003611:Dairy Products / Q000032:analysis; D005260:Female; D005504:Food Analysis / Q000379:methods*; D006801:Humans; D007118:Immunoassay; D059625:Liquid-Liquid Extraction; D013058:Mass Spectrometry; D008892:Milk / Q000737:chemistry*; D008895:Milk, Human / Q000737:chemistry*; D012680:Sensitivity and Specificity; D052616:Solid Phase Extraction; D014807:Vitamin D / Q000032:analysis*; D014815:Vitamins / Q000032:analysis*
chemical_list: D014815:Vitamins; D014807:Vitamin D
keywords: Breast milk; LC–MS; LC–UV; Sample preparation; Vitamin D