title: Cloning and expression analysis of prohibitin mRNA in canine mammary tumors.
abstract: Prohibitin is an antiproliferative protein that is a product of a putative tumor suppressor gene. However, there is little information on prohibitins in companion animals. In this study, we cloned canine prohibitin mRNA using RT-PCR and 3'-RACE (Rapid Amplification of cDNA Ends). The sequence was well conserved compared with those of other mammals, including human. The deduced amino acid sequence translated from the open reading frame completely corresponded to the human sequence. Canine prohibitin mRNA was expressed in all normal mammary and tumor samples examined. These results suggest that this protein plays a vital role in cell growth mechanisms and may be related to the occurrence of canine mammary tumors.
authors.affiliation: Laboratory of Veterinary Radiology, Division of Veterinary Sciences, Osaka Prefecture University, 1-58 Rinku-ohraikita, Izumisano Osaka 598-8531, Japan.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Matsuyama
authors.forename: Yuko
authors.initials: Y
authors.lastname: Nakano
authors.forename: Mieko
authors.initials: M
authors.lastname: Nakamura
authors.forename: Ryohei
authors.initials: R
authors.lastname: Yamamoto
authors.forename: Terumasa
authors.initials: T
authors.lastname: Shimada
authors.forename: Fumihito
authors.initials: F
authors.lastname: Ohashi
authors.forename: Kihei
authors.initials: K
authors.lastname: Kubo
mesh_terms: D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004283:Dog Diseases / Q000378:metabolism*; D004285:Dogs; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000502:physiology*; D015674:Mammary Neoplasms, Animal / Q000378:metabolism*; D008969:Molecular Sequence Data; D000091402:Prohibitins; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D000091402:Prohibitins; D012333:RNA, Messenger; D012097:Repressor Proteins