title: [Effect of AZD8330 on proliferation and apoptosis of multiple myeloma cells].
abstract: This study was aimed to investigate the effect of MEK inhibitor AZD8330 on proliferation and apoptosis of multiple myeloma IM9 and NCI-H929 cell lines and its possible mechanism. These two cell line cells were exposed to different concentrations of AZD8330 for 48 h. The CCK-8 assay was used to detect cell viability and the IC50 value at 48 h. These above-mentioned IM9 and NCI-H929 cells were treated with 5,10 and 100 nmol/L of AZD8330, then the change of cell cycle was analysed by flow cytometry with PI staining. The Wester blot was used to detect the expression levels of cyclin D and cyclin E, and multiple myeloma cells were treated with 10, 100, 1000 and 2000 nmol/L of AZD8330, the AnnexinV/7-AAD double staining was used to analyse cell apoptosis and the Western blot was used to detect the expression level of caspase-3. The results showed that AZD8330 could significantly inhibit the cell viability of IM9 and NCI-H929 cell lines in a time-and dose-dependent manner, the IC50 value (48 h) of IM9 and NCI-H929 were 19.88 ± 2.7 nmol/L and 29.3 ± 2.03 nmol/L respectively, these two cell lines were arrested on G1 phase of cell cycle, the apoptosis cells increased along with enhancement of AZD8330 concentration, and the expression level of cleaved caspase-3 protein was up-regulated. It is concluded that AZD8330 can efficiently inhibit the proliferation of NCI-H929 and IM9 cell lines, and induce apoptosis, suggesting that the AZD8330 may be a potential chemotherapeutic candidate for multiple myeloma therapy.
authors.affiliation: Department of Hematology, the Affiliated Hospital of Xuzhou Medical College, Xuzhou 221006, Jiangsu Province, China.
authors.forename: Yao
authors.initials: Y
authors.lastname: Yao
authors.affiliation: Department of Hematology, the Affiliated Hospital of Xuzhou Medical College, Xuzhou 221006, Jiangsu Province, China.
authors.forename: Yue-Ping
authors.initials: YP
authors.lastname: Bian
authors.affiliation: Department of Hematology, the Affiliated Hospital of Xuzhou Medical College, Xuzhou 221006, Jiangsu Province, China.
authors.forename: Dan-Dan
authors.initials: DD
authors.lastname: Xia
authors.affiliation: Department of Hematology, the Affiliated Hospital of Xuzhou Medical College, Xuzhou 221006, Jiangsu Province, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Pan
authors.affiliation: Department of Hematology, the Affiliated Hospital of Xuzhou Medical College, Xuzhou 221006, Jiangsu Province, China.
authors.forename: Ming-Shan
authors.initials: MS
authors.lastname: Niu
authors.affiliation: Department of Hematology, the Affiliated Hospital of Xuzhou Medical College, Xuzhou 221006, Jiangsu Province, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Zhao
authors.affiliation: Department of Hematology, the Affiliated Hospital of Xuzhou Medical College, Xuzhou 221006, Jiangsu Province, China.
authors.forename: Ling-Yu
authors.initials: LY
authors.lastname: Zeng
authors.affiliation: Department of Hematology, the Affiliated Hospital of Xuzhou Medical College, Xuzhou 221006, Jiangsu Province, China. E-mail: kailinxu_xzmc@126.com.
authors.forename: Kai-Lin
authors.initials: KL
authors.lastname: Xu
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D053148:Caspase 3; D002453:Cell Cycle; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D019927:Cyclin E; D004095:Dihydropyridines / Q000494:pharmacology*; D006801:Humans; D009101:Multiple Myeloma / Q000473:pathology*; D015513:Oncogene Proteins; D047428:Protein Kinase Inhibitors / Q000494:pharmacology*
chemical_list: C581956:AZD8330; C475820:CCNE1 protein, human; D019927:Cyclin E; D004095:Dihydropyridines; D015513:Oncogene Proteins; D047428:Protein Kinase Inhibitors; D053148:Caspase 3