title: D-sorbose inhibits disaccharidase activity and demonstrates suppressive action on postprandial blood levels of glucose and insulin in the rat.
abstract: In an attempt to develop D-sorbose as a new sweetener that could help in preventing lifestyle-related diseases, we investigated the inhibitory effect of D-sorbose on disaccharidase activity, using the brush border membrane vesicles of rat small intestines. The inhibitory effect was compared with that of L-sorbose and other rare sugars, and the small intestinal disaccharidases in rats was compared with that of humans as well. In humans and the small intestines of rats, d-sorbose strongly inhibited sucrase activity and weakly inhibited maltase activity. Inhibition by D-sorbose of sucrase activity was similar to that of L-arabinose, and the K(i) of D-sorbose was 7.5 mM. Inhibition by D-sorbose was very strong in comparison with that of L-sorbose (K(i), 60.8 mM), whereas inhibition of d-tagatose was between that of D-sorbose and L-sorbose. The inhibitory mode of D-sorbose for sucrose and maltase was uncompetitive, and that of L-sorbose was competitive. To determine a suppressive effect on postprandial blood levels of glucose and insulin via inhibition of sucrase activity, sucrose solution with or without D-sorbose was administered to rats. Increments in the blood levels of glucose and insulin were suppressed significantly after administration of sucrose solution with D-sorbose to rats, in comparison to administration of sucrose solution without D-sorbose. In contrast, the suppressive effect of L-sorbose on postprandial blood levels of glucose and insulin was very weak. These results suggest that D-sorbose may have an inhibitory effect on disaccharidase activity and could be used as a sweetener to suppress the postprandial elevation of blood levels of glucose and insulin. The use of D-sorbose as a sweetener may contribute to the prevention of lifestyle-related diseases, such as type 2 diabetes mellitus.
authors.affiliation: Graduate School of Human Health Science, University of Nagasaki Siebold, Nagayo, Nagasaki 851-2195, Japan; Faculty of Nursing and Nutrition, Department of Nutrition, University of Nagasaki Siebold, Nagayo, Nagasaki 851-2195, Japan; Institute of Food, Nutrition and Health, Jumonji University, Niiza, Saitama 352-8510, Japan. Electronic address: t-oku@jumonji-u.ac.jp.
authors.forename: Tsuneyuki
authors.initials: T
authors.lastname: Oku
authors.affiliation: Faculty of Nursing and Nutrition, Department of Nutrition, University of Nagasaki Siebold, Nagayo, Nagasaki 851-2195, Japan.
authors.forename: Yoko
authors.initials: Y
authors.lastname: Murata-Takenoshita
authors.affiliation: Institute of Food, Nutrition and Health, Jumonji University, Niiza, Saitama 352-8510, Japan.
authors.forename: Yuko
authors.initials: Y
authors.lastname: Yamazaki
authors.affiliation: Institute of Food, Nutrition and Health, Jumonji University, Niiza, Saitama 352-8510, Japan.
authors.forename: Fumio
authors.initials: F
authors.lastname: Shimura
authors.affiliation: Graduate School of Human Health Science, University of Nagasaki Siebold, Nagayo, Nagasaki 851-2195, Japan; Faculty of Nursing and Nutrition, Department of Nutrition, University of Nagasaki Siebold, Nagayo, Nagasaki 851-2195, Japan; Institute of Food, Nutrition and Health, Jumonji University, Niiza, Saitama 352-8510, Japan.
authors.forename: Sadako
authors.initials: S
authors.lastname: Nakamura
mesh_terms: D000818:Animals; D001786:Blood Glucose / Q000378:metabolism*; D003921:Diabetes Mellitus, Experimental / Q000517:prevention & control; D004186:Disaccharidases / Q000037:antagonists & inhibitors* / Q000378:metabolism; D006601:Hexoses / Q000494:pharmacology; D006801:Humans; D007328:Insulin / Q000097:blood*; D007421:Intestine, Small / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D009765:Obesity / Q000517:prevention & control; D019518:Postprandial Period; D051381:Rats; D017208:Rats, Wistar; D013013:Sorbose / Q000494:pharmacology*
chemical_list: D001786:Blood Glucose; D006601:Hexoses; D007328:Insulin; D004186:Disaccharidases; D013013:Sorbose; C030192:tagatose
keywords: Human; Inhibitory effect; Postprandial blood glucose; Rat disaccharidase; Suppressive effect; d-sorbose