title: Unravelling the transcriptome profile of the Swine respiratory tract mycoplasmas.
abstract: The swine respiratory ciliary epithelium is mainly colonized by Mycoplasma hyopneumoniae, Mycoplasma flocculare and Mycoplasma hyorhinis. While colonization by M. flocculare is virtually asymptomatic, M. hyopneumoniae and M. hyorhinis infections may cause respiratory disease. Information regarding transcript structure and gene abundance provides valuable insight into gene function and regulation, which has not yet been analyzed on a genome-wide scale in these Mycoplasma species. In this study, we report the construction of transcriptome maps for M. hyopneumoniae, M. flocculare and M. hyorhinis, which represent data for conducting comparative studies on the transcriptional repertory. For each species, three cDNA libraries were generated, yielding averages of 415,265, 695,313 and 93,578 reads for M. hyopneumoniae, M. flocculare and M. hyorhinis, respectively, with an average read length of 274 bp. The reads mapping showed that 92%, 98% and 96% of the predicted genes were transcribed in the M. hyopneumoniae, M. flocculare and M. hyorhinis genomes, respectively. Moreover, we showed that the majority of the genes are co-expressed, confirming the previously predicted transcription units. Finally, our data defined the RNA populations in detail, with the map transcript boundaries and transcription unit structures on a genome-wide scale.
authors.affiliation: Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Brazil; Programa de Pós-Graduação em Ciências Biológicas - Bioquímica, UFRGS, Porto Alegre, Brazil.
authors.forename: Franciele Maboni
authors.initials: FM
authors.lastname: Siqueira
authors.affiliation: Laboratório de Bioinformática, Laboratório Nacional de Computação Científica (LNCC), Petrópolis, Rio de Janeiro, Brazil.
authors.forename: Alexandra Lehmkuhl
authors.initials: AL
authors.lastname: Gerber
authors.affiliation: Laboratório de Bioinformática, Laboratório Nacional de Computação Científica (LNCC), Petrópolis, Rio de Janeiro, Brazil.
authors.forename: Rafael Lucas Muniz
authors.initials: RL
authors.lastname: Guedes
authors.affiliation: Laboratório de Bioinformática, Laboratório Nacional de Computação Científica (LNCC), Petrópolis, Rio de Janeiro, Brazil.
authors.forename: Luiz Gonzaga
authors.initials: LG
authors.lastname: Almeida
authors.affiliation: Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Brazil; Departamento de Biologia Molecular e Biotecnologia, Instituto de Biociências, UFRGS, Porto Alegre, Brazil.
authors.forename: Irene Silveira
authors.initials: IS
authors.lastname: Schrank
authors.affiliation: Laboratório de Bioinformática, Laboratório Nacional de Computação Científica (LNCC), Petrópolis, Rio de Janeiro, Brazil.
authors.forename: Ana Tereza Ribeiro
authors.initials: AT
authors.lastname: Vasconcelos
authors.affiliation: Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Brazil; Departamento de Biologia Molecular e Biotecnologia, Instituto de Biociências, UFRGS, Porto Alegre, Brazil.
authors.forename: Arnaldo
authors.initials: A
authors.lastname: Zaha
mesh_terms: D018829:Adhesins, Bacterial / Q000235:genetics; D000818:Animals; D002874:Chromosome Mapping; D019295:Computational Biology / Q000379:methods; D018076:DNA, Complementary; D015964:Gene Expression Regulation, Bacterial; D016680:Genome, Bacterial; D023281:Genomics; D059014:High-Throughput Nucleotide Sequencing; D009174:Mycoplasma / Q000235:genetics*; D009175:Mycoplasma Infections / Q000662:veterinary*; D022661:RNA, Untranslated / Q000235:genetics; D012141:Respiratory Tract Infections / Q000662:veterinary*; D013552:Swine; D013553:Swine Diseases / Q000382:microbiology*; D014158:Transcription, Genetic; D059467:Transcriptome
chemical_list: D018829:Adhesins, Bacterial; D018076:DNA, Complementary; D022661:RNA, Untranslated