title: Role of dietary fish oil on nitric oxide synthase activity and oxidative status in mice red blood cells.
abstract: The consumption of n-3 polyunsaturated fatty acids (PUFAs) derived from fish oil concomitant with a reduced intake of saturated fats is associated with cardiovascular benefits, which may result from the participation of nitric oxide (NO). In contrast, PUFAs are vulnerable to peroxidation, which could affect the oxidative stability of the cell and reduce NO bioavailability. Therefore, we investigated the effects of high fat diets with increasing amounts of fish oil (0-40% of energy) in place of lard on the l-arginine-NO pathway, the arginase pathway and oxidative status in mice red blood cells (RBC). We found that l-arginine transport, as well as NO synthase (NOS) expression and activity, was enhanced by the highest doses of fish oil (30 and 40%). In contrast, diets rich in lard led to NOS expression and activity impairment. Arginase expression was not significantly affected by any of the dietary regimens. No significant difference in protein and lipid oxidative markers was observed among any of the fish-oil fed mice; only lard feeding induced protein damage in addition to a decreased superoxide dismutase activity. These data suggest that a substantial dose of fish oil, but not low doses, activates the RBC l-arginine-NO pathway without resulting in oxidative damage.
authors.affiliation: Department of Pharmacology and Psychobiology, Biomedical Center, Institute of Biology, State University of Rio de Janeiro, Av 28 de Setembro 87, Rio de Janeiro, RJ 20551-030, Brazil. marcelaanjosm@gmail.com.
authors.forename: Marcela A
authors.initials: MA
authors.lastname: Martins
authors.forename: Monique B
authors.initials: MB
authors.lastname: Moss
authors.forename: Iara K S
authors.initials: IK
authors.lastname: Mendes
authors.forename: Márcia B
authors.initials: MB
authors.lastname: Águila
authors.forename: Carlos Alberto
authors.initials: CA
authors.lastname: Mandarim-de-Lacerda
authors.forename: Tatiana M C
authors.initials: TM
authors.lastname: Brunini
authors.forename: Antônio Cláudio
authors.initials: AC
authors.lastname: Mendes-Ribeiro
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001119:Arginase / Q000378:metabolism; D001120:Arginine / Q000378:metabolism; D004912:Erythrocytes / Q000201:enzymology / Q000378:metabolism*; D015525:Fatty Acids, Omega-3 / Q000494:pharmacology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D019001:Nitric Oxide Synthase / Q000378:metabolism*; D018384:Oxidative Stress / Q000187:drug effects*
chemical_list: D000975:Antioxidants; D015525:Fatty Acids, Omega-3; D001120:Arginine; D019001:Nitric Oxide Synthase; D001119:Arginase