title: Interaction between Campylobacter and intestinal epithelial cells leads to a different proinflammatory response in human and porcine host.
abstract: Campylobacter jejuni and Campylobacter coli are recognized as the leading causes of human diarrheal disease throughout the development world. Unlike human beings, gastrointestinal tract of pigs are frequently colonized by Campylobacter to a high level in a commensal manner. The aim of this study was to identify the differences underlying the divergent outcome following Campylobacter challenge in porcine versus human host. In order to address this, a comparative in vitro infection model was combined with microscopy, gentamicin protection assay, ELISA and quantitative PCR techniques. Invasion assays revealed that Campylobacter invaded human cells up to 10-fold more than porcine cells (p<0.05). In addition, gene expression of proinflammatory genes encoding for IL1α, IL6, IL8, CXCL2 and CCL20 were strongly up-regulated by Campylobacter in human epithelial cell at early times of infection, whereas a very reduced cytokine gene expression was detected in porcine epithelial cells. These data indicate that Campylobacter fails to invade porcine cells compared to human cells, and this leads to a lack of proinflammatory response induction, probably due to its pathogenic or commensal behavior in human and porcine host, respectively.
authors.affiliation: Grupo de Genómica y Mejora Animal, Departamento de Genética, Facultad de Veterinaria, Universidad de Córdoba, Campus de Rabanales, Edificio Gregor Mendel C5, 14071 Córdoba, Spain.
authors.forename: Carmen
authors.initials: C
authors.lastname: Aguilar
authors.affiliation: Grupo de Genómica y Mejora Animal, Departamento de Genética, Facultad de Veterinaria, Universidad de Córdoba, Campus de Rabanales, Edificio Gregor Mendel C5, 14071 Córdoba, Spain.
authors.forename: Ángeles
authors.initials: Á
authors.lastname: Jiménez-Marín
authors.affiliation: Grupo de Genómica y Mejora Animal, Departamento de Genética, Facultad de Veterinaria, Universidad de Córdoba, Campus de Rabanales, Edificio Gregor Mendel C5, 14071 Córdoba, Spain.
authors.forename: Rodrigo Prado
authors.initials: RP
authors.lastname: Martins
authors.affiliation: Grupo de Genómica y Mejora Animal, Departamento de Genética, Facultad de Veterinaria, Universidad de Córdoba, Campus de Rabanales, Edificio Gregor Mendel C5, 14071 Córdoba, Spain. Electronic address: ge1gapaj@uco.es.
authors.forename: Juan J
authors.initials: JJ
authors.lastname: Garrido
mesh_terms: D000818:Animals; D002167:Campylobacter / Q000235:genetics / Q000276:immunology*; D002169:Campylobacter Infections / Q000276:immunology / Q000382:microbiology / Q000662:veterinary*; D002460:Cell Line; D016207:Cytokines / Q000235:genetics / Q000276:immunology*; D004847:Epithelial Cells / Q000276:immunology / Q000382:microbiology; D054884:Host-Pathogen Interactions / Q000276:immunology; D006801:Humans; D018613:Microscopy, Confocal / Q000662:veterinary; D008855:Microscopy, Electron, Scanning / Q000662:veterinary; D012329:RNA, Bacterial / Q000737:chemistry / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D018709:Statistics, Nonparametric; D013552:Swine / Q000276:immunology / Q000382:microbiology*
chemical_list: D016207:Cytokines; D012329:RNA, Bacterial
keywords: Campylobacter coli; Campylobacter jejuni; Cellular invasion; Host–pathogen interaction; Intestinal epithelial cells