title: [BMMSC from blastic phase CML down-regulate leukemia cell apoptosis].
abstract: The purpose of this study was to investigate the effect of bone marrow mesenchymal stem cells (BMMSC) from patients with chronic myeloid leukemia (CML) in blastic phase (Bp) on K562 cells and the primary CML-Bp cells, and to explore its potential mechanisms. K562 cells and primary CML-Bp cells were co-cultured with BMMSC of different groups; the cell proliferation was detected by MTT method, the cell apoptosis rate and mitochondrial membrane potential were measured by flow cytometry, the expression levels of Caspase-8, Caspase-9, and activated Caspase-3 in cells were measured by Western blot. The results showed that the CML-Bp BMMSC could enhance the survival rate of K562 cells treated with adviamycin (ADM) and display protective effect on K562 cells and primary CML-Bp mononuctear cells, inhibited ADM-induced leukimia cell apoptosis (P < 0.05); as compared with CML-chronic phase (CML-Cp) BMMSC and normal BMMSC, the CML-Bp BMMSC showed the highest protective effect on leukemic cells, the mitochondrial membrane potential of co-cultured cells slightly droped (P < 0.05). In the CML-Bp BMMSC cultured with K562 cells, the expression level of caspase-3 was more down-regulated than that in K562 alone plus ADM group, while the expression of caspase-9 significantly increased (P < 0.05). It is concluded that the CML-Bp BMMSC down-regulates ADM-induced leukemia cell appoptosis, its mechanism may relate with the inhibition of mitochondrial membrane potential drop, the stabilization of unactive expression of caspase-9 and down-regulation of caspase-3 expression.
authors.affiliation: Departments of Hematology, The Second Hospital of Hebei Medical University, Shijiazhuang 050000, Hebei Province, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Immunology and Rheumatology, The Second Hospital of Hebei Medical University, Shijiazhuang 050000, Hebei Province, China.
authors.forename: Yu-Xiang
authors.initials: YX
authors.lastname: Han
authors.affiliation: Departments of Hematology, The Second Hospital of Hebei Medical University, Shijiazhuang 050000, Hebei Province, China.
authors.forename: Zhi-Yun
authors.initials: ZY
authors.lastname: Niu
authors.affiliation: Departments of Hematology, The Second Hospital of Hebei Medical University, Shijiazhuang 050000, Hebei Province, China.
authors.forename: Xing-Zhe
authors.initials: XZ
authors.lastname: Wang
authors.affiliation: Departments of Hematology, The Second Hospital of Hebei Medical University, Shijiazhuang 050000, Hebei Province, China.
authors.forename: Huan
authors.initials: H
authors.lastname: Hua
authors.affiliation: Departments of Hematology, The Second Hospital of Hebei Medical University, Shijiazhuang 050000, Hebei Province, China.
authors.forename: Yin-Tao
authors.initials: YT
authors.lastname: Shang
authors.affiliation: Departments of Hematology, The Second Hospital of Hebei Medical University, Shijiazhuang 050000, Hebei Province, China.
authors.forename: Fu-Xu
authors.initials: FX
authors.lastname: Wang
authors.affiliation: Departments of Hematology, The Second Hospital of Hebei Medical University, Shijiazhuang 050000, Hebei Province, China.
authors.forename: Xue-Jun
authors.initials: XJ
authors.lastname: Zhang
authors.affiliation: Departments of Hematology, The Second Hospital of Hebei Medical University, Shijiazhuang 050000, Hebei Province, China. E-mail: luojianmin2014@126.com.
authors.forename: Jian-Min
authors.initials: JM
authors.lastname: Luo
mesh_terms: D017209:Apoptosis; D001853:Bone Marrow; D053148:Caspase 3; D053181:Caspase 8; D053453:Caspase 9; D049109:Cell Proliferation; D002478:Cells, Cultured; D015536:Down-Regulation; D006801:Humans; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000473:pathology*; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism
chemical_list: D053148:Caspase 3; D053181:Caspase 8; D053453:Caspase 9