title: Immunomodulatory activity in vitro and in vivo of verbascose from mung beans (Phaseolus aureus).
abstract: In the present study, the immunostimulatory activity of verbascose from mung beans (Phaseolus aureus) was evaluated by using in vitro cell models and in vivo animal models. The results of in vitro experiments showed that verbascose could enhance the ability of devouring neutral red of peritoneal macrophages and promote the release of nitric oxide and immune reactive molecules such as interleukin (IL)-6, IL-1β, interferon (IFN)-α, and IFN-γ. Treatment with verbascose at a dose of 200 μg/mL exhibited the best effects. For assay in vivo, administration of verbascose at a medium dose of 90 mg/kg body weight could significantly increase the index of spleen, activity of lysozyme in spleen and serum, hemolysin level in serum, and swelling rate of earlap in the delayed type of hypersensitivity (DTH) of immunosuppressed mice. All of the results suggested that verbascose had potent immunostimulatory activity and could be explored as a potential natural immunomodulatory agent in functional foods.
authors.affiliation: College of Food Science and Technology, Nanjing Agricultural University , Nanjing 210095, People's Republic of China.
authors.forename: Zhuqing
authors.initials: Z
authors.lastname: Dai
authors.forename: Di
authors.initials: D
authors.lastname: Su
authors.forename: Yun
authors.initials: Y
authors.lastname: Zhang
authors.forename: Yi
authors.initials: Y
authors.lastname: Sun
authors.forename: Bing
authors.initials: B
authors.lastname: Hu
authors.forename: Hong
authors.initials: H
authors.lastname: Ye
authors.forename: Saqib
authors.initials: S
authors.lastname: Jabbar
authors.forename: Xiaoxiong
authors.initials: X
authors.lastname: Zeng
mesh_terms: D000818:Animals; D002460:Cell Line; D055951:Functional Food / Q000032:analysis; D006968:Hypersensitivity, Delayed / Q000188:drug therapy* / Q000276:immunology; D007155:Immunologic Factors / Q000008:administration & dosage*; D053583:Interleukin-1beta / Q000276:immunology; D015850:Interleukin-6 / Q000276:immunology; D017737:Macrophages, Peritoneal / Q000187:drug effects / Q000276:immunology; D051379:Mice; D009569:Nitric Oxide / Q000276:immunology; D009844:Oligosaccharides / Q000008:administration & dosage*; D027805:Phaseolus / Q000737:chemistry*; D010936:Plant Extracts / Q000008:administration & dosage*; D012639:Seeds / Q000737:chemistry
chemical_list: D007155:Immunologic Factors; D053583:Interleukin-1beta; D015850:Interleukin-6; D009844:Oligosaccharides; D010936:Plant Extracts; D009569:Nitric Oxide; C570815:verbascose
keywords: immunomodulating activity; in vitro; in vivo; mung beans (Phaseolus aureus); verbascose