title: Cytosine-bulge-dependent fluorescence quenching for the real-time hairpin primer PCR.
abstract: The progress of a polymerase chain reaction (PCR) was sensitively monitored based on the increase in fluorescence of N,N'-bis(3-aminopropyl)-2,7-diamino-1,8-naphthyridine, which was covalently anchored on the cytosine bulge directly neighbouring the 5'-T_G-3'/5'-CCA-3' sequence in the hairpin tag at the 5' end of the PCR primer.
authors.affiliation: The Institute of Scientific and Industrial Research, Osaka University, 8-1 Mihogaoka, Ibaraki, 567-0047 Japan. nakatani@sanken.osaka-u.ac.jp.
authors.forename: F
authors.initials: F
authors.lastname: Takei
authors.forename: X
authors.initials: X
authors.lastname: Chen
authors.forename: G
authors.initials: G
authors.lastname: Yu
authors.forename: T
authors.initials: T
authors.lastname: Shibata
authors.forename: C
authors.initials: C
authors.lastname: Dohno
authors.forename: K
authors.initials: K
authors.lastname: Nakatani
mesh_terms: D003596:Cytosine / Q000737:chemistry*; D017931:DNA Primers / Q000737:chemistry / Q000378:metabolism*; D005456:Fluorescent Dyes / Q000737:chemistry*; D009287:Naphthyridines / Q000737:chemistry; D010957:Plasmids / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D017931:DNA Primers; D005456:Fluorescent Dyes; D009287:Naphthyridines; D003596:Cytosine