title: High frame-rate multichannel beam-scanning microscopy based on Lissajous trajectories.
abstract: A simple beam-scanning optical design based on Lissajous trajectory imaging is described for achieving up to kHz frame-rate optical imaging on multiple simultaneous data acquisition channels. In brief, two fast-scan resonant mirrors direct the optical beam on a circuitous trajectory through the field of view, with the trajectory repeat-time given by the least common multiplier of the mirror periods. Dicing the raw time-domain data into sub-trajectories combined with model-based image reconstruction (MBIR) 3D in-painting algorithms allows for effective frame-rates much higher than the repeat time of the Lissajous trajectory. Since sub-trajectory and full-trajectory imaging are simply different methods of analyzing the same data, both high-frame rate images with relatively low resolution and low frame rate images with high resolution are simultaneously acquired. The optical hardware required to perform Lissajous imaging represents only a minor modification to established beam-scanning hardware, combined with additional control and data acquisition electronics. Preliminary studies based on laser transmittance imaging and polarization-dependent second harmonic generation microscopy support the viability of the approach both for detection of subtle changes in large signals and for trace-light detection of transient fluctuations.
authors.forename: Shane Z
authors.initials: SZ
authors.lastname: Sullivan
authors.forename: Ryan D
authors.initials: RD
authors.lastname: Muir
authors.forename: Justin A
authors.initials: JA
authors.lastname: Newman
authors.forename: Mark S
authors.initials: MS
authors.lastname: Carlsen
authors.forename: Suhas
authors.initials: S
authors.lastname: Sreehari
authors.forename: Chris
authors.initials: C
authors.lastname: Doerge
authors.forename: Nathan J
authors.initials: NJ
authors.lastname: Begue
authors.forename: R Michael
authors.initials: RM
authors.lastname: Everly
authors.forename: Charles A
authors.initials: CA
authors.lastname: Bouman
authors.forename: Garth J
authors.initials: GJ
authors.lastname: Simpson
mesh_terms: D000465:Algorithms; D003952:Diagnostic Imaging; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D008859:Microscopy, Polarization / Q000379:methods*; D008962:Models, Theoretical; D019047:Phantoms, Imaging