title: Purification and Characterization of a Fibrinolytic Enzyme from Bacillus pumilus 2.g Isolated from Gembus, an Indonesian Fermented Food.
abstract: Bacillus pumilus 2.g isolated from gembus, an Indonesian fermented soybean cake, secretes several proteases that have strong fibrinolytic activities. A fibrinolytic enzyme with an apparent molecular weight of 20 kDa was purified from the culture supernatant of B. pumilus 2.g by sequential application of ammonium sulfate precipitation, ion-exchange chromatography, and hydrophobic chromatography. The partially purified enzyme was stable between pH 5 and pH 9 and temperature of less than 60°C. Fibrinolytic activity was increased by 5 mM MgCl2 and 5 mM CaCl2 but inhibited by 1 mM phenylmethylsulfonyl fluoride (PMSF), 1 mM sodium dodecyl sulfate (SDS), and 1 mM ethylenediaminetetraacetic acid (EDTA). The partially purified enzyme quickly degraded the α and β chains of fibrinogen but was unable to degrade the γ chain.
authors.affiliation: Department of Nutrition Science, Diponegoro University, Semarang 50231, Central Java, Indonesia ; Department of Food Science and Technology, Bogor Agricultural University, Bogor 16680, West Java, Indonesia.
authors.forename: Diana Nur
authors.initials: DN
authors.lastname: Afifah
authors.affiliation: Department of Nutrition Science, Diponegoro University, Semarang 50231, Central Java, Indonesia.
authors.forename: Muhammad
authors.initials: M
authors.lastname: Sulchan
authors.affiliation: Department of Food Science and Technology, Bogor Agricultural University, Bogor 16680, West Java, Indonesia.
authors.forename: Dahrul
authors.initials: D
authors.lastname: Syah
authors.affiliation: Faculty of Biotechnology, Atma Jaya Catholic University of Indonesia, Jakarta 12930, Indonesia.
authors.lastname: Yanti
authors.affiliation: Department of Food Science and Technology, Bogor Agricultural University, Bogor 16680, West Java, Indonesia.
authors.forename: Maggy Thenawidjaja
authors.initials: MT
authors.lastname: Suhartono
authors.affiliation: Division of Applied Life Science (BK21 Plus), Graduate School, Gyeongsang National University, Gyeongnam 660-701, Korea.
authors.forename: Jeong Hwan
authors.initials: JH
authors.lastname: Kim
keywords: Bacillus pumilus; fermented food; fibrinolytic enzyme; gembus