title: Molecular reprogramming of Arabidopsis in response to perturbation of jasmonate signaling.
abstract: Jasmonates (JAs) are important phytohormones that regulate a wide range of plant processes including growth, development, senescence, and defense. Jasmonate ZIM-domain (JAZ) proteins are repressors in JA signaling. In Arabidopsis thaliana, 12 JAZ encoding genes were identified, but only a few have been studied in detail. In this study, we focused on characterizing the molecular networks involving JAZ2 and JAZ7. To understand the phenotypes and elucidate the regulatory functions of JAZ2 and JAZ7, shoot and root tissues from wild type (WT), jaz2, and jaz7 were harvested for RNA sequencing and metabolomics. Distinct changes of transcripts and metabolites in JA biosynthesis, primary and specialized metabolism, and oxidative stress were observed among the three genotypes. In particular, many defense or stress-associated metabolites and specialized metabolites were increased in response to methyl jasmonate (MeJA) treatment. Most importantly, these changes were subjected to quantitative modulation by the JAZ proteins at both transcriptional and metabolic levels, the degree of which may control resource allocation between growth and defense. This study not only reveals MeJA-induced molecular reprogramming but also demonstrates the functions of JAZ proteins as key regulators in fine-tuning JA signal transduction.
authors.affiliation: Department of Biology, ‡Interdisciplinary Center for Biotechnology Research, and §Plant Molecular and Cellular Biology, University of Florida , Gainesville, Florida 32611, United States.
authors.forename: Huizhuan
authors.initials: H
authors.lastname: Yan
authors.forename: Mi-Jeong
authors.initials: MJ
authors.lastname: Yoo
authors.forename: Jin
authors.initials: J
authors.lastname: Koh
authors.forename: Lihong
authors.initials: L
authors.lastname: Liu
authors.forename: Yazhou
authors.initials: Y
authors.lastname: Chen
authors.forename: Dogukan
authors.initials: D
authors.lastname: Acikgoz
authors.forename: Qiaomei
authors.initials: Q
authors.lastname: Wang
authors.forename: Sixue
authors.initials: S
authors.lastname: Chen
mesh_terms: D000085:Acetates / Q000494:pharmacology*; D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism; D002853:Chromatography, Liquid; D003517:Cyclopentanes / Q000378:metabolism / Q000494:pharmacology*; D008401:Gas Chromatography-Mass Spectrometry; D020869:Gene Expression Profiling / Q000379:methods*; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D005947:Glucose / Q000378:metabolism; D013058:Mass Spectrometry; D055442:Metabolome / Q000187:drug effects; D055432:Metabolomics / Q000379:methods*; D008969:Molecular Sequence Data; D009154:Mutation; D054883:Oxylipins / Q000378:metabolism / Q000494:pharmacology*; D010937:Plant Growth Regulators / Q000494:pharmacology; D018517:Plant Roots / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D018520:Plant Shoots / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D015398:Signal Transduction / Q000187:drug effects; D059467:Transcriptome / Q000187:drug effects
chemical_list: D000085:Acetates; D029681:Arabidopsis Proteins; D003517:Cyclopentanes; C000602232:JAZ2 protein, Arabidopsis; C000602233:JAZ7 protein, Arabidopsis; D054883:Oxylipins; D010937:Plant Growth Regulators; D012097:Repressor Proteins; C011006:jasmonic acid; C072239:methyl jasmonate; D005947:Glucose
keywords: Arabidopsis thaliana; JAZ; jasmonate; metabolomics; molecular networks; transcriptomics