title: Direct analysis of intact proteins from Escherichia coli colonies by liquid extraction surface analysis mass spectrometry.
abstract: Top-down identification of proteins by liquid extraction surface analysis (LESA) mass spectrometry has previously been reported for tissue sections and dried blood spot samples. Here, we present a modified "contact" LESA method for top-down analysis of proteins directly from living bacterial colonies grown in Petri dishes, without any sample pretreatment. It was possible to identify a number of proteins by use of collision-induced dissociation tandem mass spectrometry followed by searches of the data against an E. coli protein database. The proteins identified suggest that the method may provide insight into the bacterial response to environmental conditions. Moreover, the results show that the "contact" LESA approach results in a smaller sampling area than typical LESA, which may have implications for spatial profiling.
authors.affiliation: School of Biosciences, University of Birmingham , Edgbaston, Birmingham, B15 2TT, United Kingdom.
authors.forename: Elizabeth C
authors.initials: EC
authors.lastname: Randall
authors.forename: Josephine
authors.initials: J
authors.lastname: Bunch
authors.forename: Helen J
authors.initials: HJ
authors.lastname: Cooper
mesh_terms: D018929:Cell Culture Techniques; D004867:Equipment Design; D004926:Escherichia coli / Q000737:chemistry*; D029968:Escherichia coli Proteins / Q000032:analysis / Q000302:isolation & purification*; D059625:Liquid-Liquid Extraction / Q000295:instrumentation*; D013058:Mass Spectrometry / Q000295:instrumentation*
chemical_list: D029968:Escherichia coli Proteins