title: High expression of MAGE-A9 correlates with unfavorable survival in hepatocellular carcinoma.
abstract: Melanoma-associated antigens (MAGE)-A9 has been reported to play important roles in the development of human cancers. However, the association between MAGE-A9 expression and the clinicopathological characteristics of hepatocellular carcinoma (HCC) is not well understood. The study was to detect the expression of MAGE-A9 in human HCC and investigate the association between its expression and the clinicopathological characteristics of HCC. Reverse transcription-polymerase chain reaction (RT-PCR), one-step quantitative -PCR (qPCR) and immunohistochemistry (IHC) analyses were performed to characterize the expression of MAGE-A9 in HCC cell lines and tissues. Kaplan-Meier survival and Cox regression analyses were employed to evaluate the prognosis of 100 HCC patients. The results showed that the expression of MAGE-A9 in HCC was significantly higher than that in non-cancerous cells and tissues. Moreover, the expression level of the MAGE-A9 protein in HCC was related to the pathological grade (p = 0.003), portal vein invasion (p = 0.001), distant metastasis (p = 0.022) and TNM stage (p = 0.005). Cox regression analysis further revealed that MAGE-A9 expression is an independent prognostic factor for disease-free survival (p = 0.006) and overall survival (p = 0.022). These data are the first to indicate that MAGE-A9 expression is a valuable prognostic biomarker for HCC and that high MAGE-A9 expression suggests unfavorable survival outcomes in HCC patients.
authors.affiliation: Department of Infectious Diseases, The First People's Hospital of Kunshan Affiliated with Jiangsu University, Suzhou 215000, China.
authors.forename: Xuefeng
authors.initials: X
authors.lastname: Gu
authors.affiliation: Department of Infectious Diseases, The First People's Hospital of Kunshan Affiliated with Jiangsu University, Suzhou 215000, China.
authors.forename: Maoying
authors.initials: M
authors.lastname: Fu
authors.affiliation: Department of Anesthesiology, Yixing People's Hospital, The Affiliated Yixing Hospital of Jiangsu University, Yixing 214200, China.
authors.forename: Zhijun
authors.initials: Z
authors.lastname: Ge
authors.affiliation: Department of Hepatobiliary and Laparoscopic Surgery, Yixing People's Hospital, The Affiliated Yixing Hospital of Jiangsu University, Yixing 214200, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Zhan
authors.affiliation: Department of Infectious Diseases, The First People's Hospital of Kunshan Affiliated with Jiangsu University, Suzhou 215000, China.
authors.forename: Yuqin
authors.initials: Y
authors.lastname: Ding
authors.affiliation: Department of Infectious Diseases, The First People's Hospital of Kunshan Affiliated with Jiangsu University, Suzhou 215000, China.
authors.forename: Huihui
authors.initials: H
authors.lastname: Ni
authors.affiliation: Department of Infectious Diseases, The First People's Hospital of Kunshan Affiliated with Jiangsu University, Suzhou 215000, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Department of Infectious Diseases, The First People's Hospital of Kunshan Affiliated with Jiangsu University, Suzhou 215000, China.
authors.forename: Yanfang
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: The Key Laboratory of Cancer Biomarkers, Prevention &Treatment Cancer Center and The Key Laboratory of Antibody Technique of Ministry of Health, Nanjing Medical University, Nanjing 210029, China.
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Tang
authors.affiliation: Department of Pathology, The Second Affiliated Hospital of Nanjing Medical University, Nanjing 210015, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Xiong
authors.affiliation: Department of Pathology, Jiangsu Province Geriatric Institute, Nanjing 210029, China.
authors.forename: Jiang
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Pathology, Jiangsu Province Geriatric Institute, Nanjing 210029, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Qiu
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, Jiangsu Provincial Hospital, Nanjing 210000, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Mao
authors.affiliation: 1] The Key Laboratory of Cancer Biomarkers, Prevention &Treatment Cancer Center and The Key Laboratory of Antibody Technique of Ministry of Health, Nanjing Medical University, Nanjing 210029, China [2] Huadong Medical Institute of Biotechniques, Nanjing 210002, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Zhu
mesh_terms: D000328:Adult; D000951:Antigens, Neoplasm / Q000096:biosynthesis* / Q000235:genetics; D006528:Carcinoma, Hepatocellular / Q000235:genetics* / Q000473:pathology; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D056945:Hep G2 Cells; D006801:Humans; D053208:Kaplan-Meier Estimate; D008113:Liver Neoplasms / Q000235:genetics* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000096:biosynthesis* / Q000235:genetics; D011379:Prognosis
chemical_list: D000951:Antigens, Neoplasm; C520253:MAGEA9 protein, human; D009363:Neoplasm Proteins