title: In vivo magnetic resonance imaging tracking of transplanted superparamagnetic iron oxide-labeled bone marrow mesenchymal stem cells in rats with myocardial infarction.
abstract: Superparamagnetic iron oxide (SPIO) nanoparticles generate superparamagnetism, thereby resulting in an inhomogeneous local magnetic field, which shortens the T2 value on magnetic resonance imaging (MRI). The purpose of the present study was to use MRI to track bone marrow mesenchymal stem cells (BMSCs) labeled with SPIO in a rat model of myocardial infarction. The BMSCs were isolated from rats and labeled with SPIO. The anterior descending branch of the coronary artery was ligated under anesthesia. Two weeks later, the rats received, at random, 5 x 10(7) SPIO-labeled BMSCs, 5 x 10(7) unlabeled BMSCs or a vehicle (100 µl phosphate-buffered saline) via direct injection into the ischemic area (20 animals/group). MRI was used to track the SPIO‑labeled BMSCs and the rats were then sacrificed to verify the presence of BMSCs using immunohistochemistry with an anti-CD90 antibody. The procedure labeled 99% of the BMSCs with SPIO, which exhibited low-intensity signals on T2 and T2* MRI imaging. At 24 h post-BMSC transplantation, low-intensity MRI signals were detected on the T2 and T2* sequences at the infarction margins. After 3 weeks following transplantation, low-intensity signals started to appear within the infarcted area; however, the signal intensity subsequently decreased and became indistinct. Immunohistochemistry revealed that the SPIO-labeled BMSCs migrated from the margin into the infarcted region. In conclusion, the BMSCs were readily labeled with SPIO and in vivo and MRI tracking demonstrated that the SPIO-labeled BMSCs established and grew in the infarcted myocardium.
authors.affiliation: Department of Cardiothoracic Surgery, The Sun Yat‑Sen Memorial Hospital, Sun Yat‑Sen University, Guangzhou, Guangdong 510120, P.R. China.
authors.forename: Ping
authors.initials: P
authors.lastname: Hua
authors.affiliation: Department of Thoracic Surgery, Sichuan Provincial People's Hospital, Chengdu, Sichuan 610072, P.R. China.
authors.forename: You-Yu
authors.initials: YY
authors.lastname: Wang
authors.affiliation: Department of Cardiothoracic Surgery, The Sun Yat‑Sen Memorial Hospital, Sun Yat‑Sen University, Guangzhou, Guangdong 510120, P.R. China.
authors.forename: Li-Bao
authors.initials: LB
authors.lastname: Liu
authors.affiliation: Department of Cardiothoracic Surgery, The Sun Yat‑Sen Memorial Hospital, Sun Yat‑Sen University, Guangzhou, Guangdong 510120, P.R. China.
authors.forename: Jia-Liang
authors.initials: JL
authors.lastname: Liu
authors.affiliation: Department of Cardiothoracic Surgery, The Sun Yat‑Sen Memorial Hospital, Sun Yat‑Sen University, Guangzhou, Guangdong 510120, P.R. China.
authors.forename: Jian-Yang
authors.initials: JY
authors.lastname: Liu
authors.affiliation: Department of Cardiothoracic Surgery, The Sun Yat‑Sen Memorial Hospital, Sun Yat‑Sen University, Guangzhou, Guangdong 510120, P.R. China.
authors.forename: Yan-Qi
authors.initials: YQ
authors.lastname: Yang
authors.affiliation: Department of Neurology, Guangzhou First Municipal People's Hospital, Guangzhou, Guangdong 510180, P.R. China.
authors.forename: Song-Ran
authors.initials: SR
authors.lastname: Yang
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques; D002469:Cell Separation; D002470:Cell Survival; D058948:Cell Tracking; D004195:Disease Models, Animal; D005260:Female; D052203:Ferrosoferric Oxide; D008279:Magnetic Resonance Imaging; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000378:metabolism*; D009203:Myocardial Infarction / Q000175:diagnosis / Q000473:pathology* / Q000628:therapy*; D051381:Rats
chemical_list: D052203:Ferrosoferric Oxide