title: Association between the hypomethylation of osteopontin and integrin β3 promoters and vascular smooth muscle cell phenotype switching in great saphenous varicose veins.
abstract: Lower extremity varicose veins are a common condition in vascular surgery and proliferation of vascular smooth muscle cells (VSMCs) in the intima is a significant pathological feature of varicosity. However, the pathogenesis of varicose veins is not fully understood. Osteopontin (OPN) could promote the migration and adhesion of VSMCs through the cell surface receptor integrin β3 and the cooperation of OPN and integrin β3 is involved in many vascular diseases. However, the role of OPN and integrin β3 in varicosity remains unclear. In the current study, we found that the methylation levels in the promoter regions of OPN and integrin β3 genes in the VSMCs of varicose veins are reduced and the protein expression of OPN and integrin β3 are increased, compared with normal veins. Furthermore, it was observed that VSMCs in the neointima of varicose veins were transformed into the synthetic phenotype. Collectively, hypomethylation of the promoter regions for OPN and integrin β3 genes may increase the expression of these genes in varicosity, which is closely related to VSMC phenotype switching. Hypomethylation of the promoter regions for OPN and integrin β3 genes may be a key factor in the pathogenesis of varicosity.
authors.affiliation: Department of Vascular and Thyroid Surgery, the First Affiliated Hospital of China Medical University, Shenyang 110001, China. cmu_jianghan@126.com.
authors.forename: Han
authors.initials: H
authors.lastname: Jiang
authors.affiliation: Department of Vascular and Thyroid Surgery, the First Affiliated Hospital of China Medical University, Shenyang 110001, China. cmu_lunyu@163.com.
authors.forename: Yu
authors.initials: Y
authors.lastname: Lun
authors.affiliation: Department of Vascular and Thyroid Surgery, the First Affiliated Hospital of China Medical University, Shenyang 110001, China. cmu_wuxiaoyu@163.com.
authors.forename: Xiaoyu
authors.initials: X
authors.lastname: Wu
authors.affiliation: Department of Vascular and Thyroid Surgery, the First Affiliated Hospital of China Medical University, Shenyang 110001, China. cmu_xiaqian@163.com.
authors.forename: Qian
authors.initials: Q
authors.lastname: Xia
authors.affiliation: Department of Vascular and Thyroid Surgery, the First Affiliated Hospital of China Medical University, Shenyang 110001, China. cmu_zhangxiaoyu@163.com.
authors.forename: Xiaoyu
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Vascular and Thyroid Surgery, the First Affiliated Hospital of China Medical University, Shenyang 110001, China. cmu_xinshijie@163.com.
authors.forename: Shijie
authors.initials: S
authors.lastname: Xin
authors.affiliation: Department of Vascular and Thyroid Surgery, the First Affiliated Hospital of China Medical University, Shenyang 110001, China. cmu_zhangjian@163.com.
authors.forename: Jian
authors.initials: J
authors.lastname: Zhang
mesh_terms: D000328:Adult; D000368:Aged; D019175:DNA Methylation; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D039661:Integrin beta3 / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D009131:Muscle, Smooth, Vascular / Q000166:cytology* / Q000473:pathology; D032389:Myocytes, Smooth Muscle / Q000378:metabolism* / Q000473:pathology; D053495:Osteopontin / Q000235:genetics*; D010641:Phenotype; D011401:Promoter Regions, Genetic; D014648:Varicose Veins / Q000235:genetics* / Q000473:pathology
chemical_list: D039661:Integrin beta3; D053495:Osteopontin