title: [Quantitative analysis of interleukin-5 mRNA and protein in nasal polyps].
abstract: OBJECTIVE
  To study the expression of intedeukin-5 (IL-5) mRNA and protein in Nasal polyps and in the inferior turbinate, and to explore the relationship between the expression and the Clinical features.
  
  
  METHODS
  Real time fluorescent quantitative PCR(RT-qPCR) and immunohistochemical staining were used to detect the expression of intedeukin-5 (IL-5) mRNA and protein in nasal polyps of 24 cases and in inferior turbinate of 15 cases.
  
  
  RESULTS
  The expression of IL-5 mRNA in Nasal polyps was 7.52 times higher than that in the inferior turbinate (P < 0.01). The expression rate of IL-5 protein in Nasal polyps was 79.17%, significantly higher than that in the inferior turbinate (26.67%) (chi2 = 10.52, P < 0.01). The differece of expression of IL-5 mRNA was not associated with the sexual distinction, unilateral or bilateral nasal polyps and primary or recurrent nasal polyps (P > 0.01), but was associated with the single or multiple nasal polyps, nasal polyps with or without allergic rhinitis and/or asthma. The differece of expression of IL-5 protein was not associated with the sexual distinction, unilateral or bilateral nasal polyps, primary or recurrent nasal polyps, nasal polyps with or without allergic rhinitis and/or asthma, but was associated with the single and multiple nasal polyps.
  
  
  CONCLUSIONS
  The high expression of IL-5 mRNA and protein is closely related with the formation edema and development of nasal polyps. Some clinical features of Nasal polyps related to the expression level of IL-5 in nasal polyps.
authors.forename: Anbin
authors.initials: A
authors.lastname: Zhao
authors.forename: Hua
authors.initials: H
authors.lastname: Wang
authors.forename: Hao
authors.initials: H
authors.lastname: Wu
authors.forename: Yongguo
authors.initials: Y
authors.lastname: Yang
authors.forename: Haiyan
authors.initials: H
authors.lastname: Xu
authors.forename: Daxin
authors.initials: D
authors.lastname: Wang
mesh_terms: D005260:Female; D006801:Humans; D015848:Interleukin-5 / Q000235:genetics / Q000378:metabolism*; D008297:Male; D009298:Nasal Polyps / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics; D014420:Turbinates / Q000378:metabolism
chemical_list: C508599:IL5 protein, human; D015848:Interleukin-5; D012333:RNA, Messenger