title: [The PCR markers of viral infections under chronic gastritis in children].
abstract: The extended monitoring (up to 1 year 11 months) of PCR markers was implemented concerning viral infections: cytomegalovirus, Epstein-Barr virus, simple herpes virus type I and II, hepatitis B virus, hepatitis C virus and bacterial infection of Helicobacter pylori in bioassays (blood, biopsy material of mucous coat of stomach and inferior third of esophagus) from children with different types of chronic gastritis. In biological samples from patients with gastritis type A and type A + B DNA of hepatitis B virus (87% and 71% of patients correspondingly) and DNA of Epstein-Barr virus (63% and 67% of patients) were detected with high rate. Under gastritis type B and C these markers were detected significantly rarely (20-36%). Among patients with gastritis type A, B and A + B, the positive results on DNA of cytomegalovirus consisted 13-17%. In patients with gastritis type C DNA of cytomegalovirus was not detected. In any of analyzed samples no DNA of simple herpes virus type I and II was detected. The control of DNA of H. pylori demonstrated its presence in biological materials of 67% and 84% of patients with gastritis type B and A +B. This type of DNA was absent in patients with gastritis type A and C. Under gastritis type A, B and A+B, DNA of Epstein-Barr virus and DNA of hepatitis B virus detected more often in biological materials of mucous coat of stomach (71%-100%) and out of them simultaneously in blood in 33%-60% of examined patients and only in blood up to 29%. DNA of Epstein-Barr virus was detected in leukocytes of peripheral blood and DNA of hepatitis B virus both in plasma and leukocytes of peripheral blood. Under gastritis type C DNA of Epstein-Barr virus was always detected in leukocytes of peripheral blood (in 20% out of these patients simultaneously in biological material) and DNA of hepatitis B virus just as much in blood (plasma and/or leukocytes of peripheral blood) and biological materials. The lower concentrations (less than 700 copies/ml) DNA of hepatitis B virus in most samples were detected in absence of markers of hepatitis B virus. In patients with autoimmune gastritis and in absence of bacterial infection H. pylori (group I) or against its background (group III) PCR-markers of hepatitis B virus and Epstein-Barr virus were detected quite often. The evidence of persistence (in superior sections of digestive organs) of Epstein-Barr virus nad hepatitis B virus is detection of DNA of these viruses under their extended monitoring (up to 1 year 11 months) in biological samples from patients with autoimmune forms of gastritis type A and type A+B.
authors.forename: N G
authors.initials: NG
authors.lastname: Yaroslavtseva
authors.forename: L O
authors.initials: LO
authors.lastname: Grumbkova
authors.forename: D S
authors.initials: DS
authors.lastname: Tikhomirov
authors.forename: E N
authors.initials: EN
authors.lastname: Ignatova
authors.forename: T Yu
authors.initials: TY
authors.lastname: Romanova
authors.forename: T A
authors.initials: TA
authors.lastname: Garanja
authors.forename: T A
authors.initials: TA
authors.lastname: Tupoleva
authors.forename: F P
authors.initials: FP
authors.lastname: Filatov
mesh_terms: D000293:Adolescent; D015415:Biomarkers; D002648:Child; D002675:Child, Preschool; D005260:Female; D005756:Gastritis / Q000150:complications / Q000382:microbiology / Q000821:virology*; D016481:Helicobacter Infections / Q000150:complications / Q000175:diagnosis; D006505:Hepatitis / Q000150:complications / Q000175:diagnosis*; D006566:Herpesviridae Infections / Q000150:complications / Q000175:diagnosis*; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction
chemical_list: D015415:Biomarkers