title: Simultaneous determination of water-soluble vitamins in beverages and dietary supplements by LC-MS/MS.
abstract: An LC-MS/MS method was developed for the simultaneous determination of 15 water-soluble vitamins that are widely used as additives in beverages and dietary supplements. This combined method involves the following simple pre-treatment procedures: dietary supplement samples were prepared by centrifugation and filtration after an extraction step, whereas beverage samples were diluted prior to injection. Chromatographic analysis in this method utilised a multi-mode ODS column, which provided reverse-phase, anion- and cation-exchange capacities, and therefore improved the retention of highly polar analytes such as water-soluble vitamins. Additionally, the multi-mode ODS column did not require adding ion pair reagents to the mobile phase. We optimised the chromatographic separation of 15 water-soluble vitamins by adjusting the mobile phase pH and the organic solvent. We also conducted an analysis of a NIST Standard Reference Material (SRM 3280 Multi-vitamin/Multi-element tablets) using this method to verify its accuracy. In addition, the method was applied to identify the vitamins in commercial beverages and dietary supplements. By comparing results with the label values and results obtained by official methods, it was concluded that the method could be used for quality control and to compose nutrition labels for vitamin-enriched products.
authors.affiliation: a Research Laboratories for Food Safety Chemistry , Asahi Group Holdings Ltd , Moriya , Ibaraki , Japan.
authors.forename: Ayano
authors.initials: A
authors.lastname: Kakitani
authors.forename: Tomonori
authors.initials: T
authors.lastname: Inoue
authors.forename: Keiko
authors.initials: K
authors.lastname: Matsumoto
authors.forename: Jun
authors.initials: J
authors.lastname: Watanabe
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Nagatomi
authors.forename: Naoki
authors.initials: N
authors.lastname: Mochizuki
mesh_terms: D001205:Ascorbic Acid / Q000032:analysis / Q000737:chemistry; D001628:Beverages / Q000032:analysis*; D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D019587:Dietary Supplements / Q000032:analysis*; D005513:Food Inspection / Q000379:methods*; D005515:Food Labeling; D005527:Food, Fortified / Q000032:analysis*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007564:Japan; D009753:Nutritive Value; D011786:Quality Control; D015203:Reproducibility of Results; D012995:Solubility; D012997:Solvents / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry; D014803:Vitamin B Complex / Q000032:analysis / Q000737:chemistry; D014815:Vitamins / Q000032:analysis* / Q000737:chemistry
chemical_list: D012997:Solvents; D014815:Vitamins; D014803:Vitamin B Complex; D001205:Ascorbic Acid
keywords: LC-MS/MS; multi-mode ODS column; nutrition labelling; quality control; water-soluble vitamin