title: Surface-enhanced Raman scattering aptasensor for ultrasensitive trace analysis of bisphenol A.
abstract: Surface-enhanced Raman scattering (SERS)-based aptasensor platform, using double strand DNA-embedded Au/Ag core-shell nanoparticles, has been developed for the ultrasensitive detection of bisphenol A (BPA) in water. By combining optimally controlled Au/Ag core-shell nanoparticles with the selective BPA binding characteristics of DNA aptamers, a highly sensitive limit of detection (LOD) of 10 fM could be achieved for BPA-spiked tap water over a wide concentration range from 100 nM to 10 fM. This LOD is two or three orders of magnitude lower than that reported for other BPA sensing techniques, and also yields a detection limit that is 100-1000 times lower than the US EPA-defined Predicted No Effect Concentration (PNEC) values in potable water. Total detection time is estimated to be about 40 min including the reaction between aptamer and BPA (30 min) and detection (10 min). This sensing platform is also suitable for field applications since measurement can be performed under aqueous colloidal conditions.
authors.affiliation: Department of Bionano Technology, Hanyang University, Ansan 426-791, South Korea.
authors.forename: Eunsu
authors.initials: E
authors.lastname: Chung
authors.affiliation: Department of Bionano Technology, Hanyang University, Ansan 426-791, South Korea.
authors.forename: Jinhyeok
authors.initials: J
authors.lastname: Jeon
authors.affiliation: Department of Bionano Technology, Hanyang University, Ansan 426-791, South Korea.
authors.forename: Jimin
authors.initials: J
authors.lastname: Yu
authors.affiliation: Department of Electronics & Communication Engineering, Hanyang University, Ansan 426-791, South Korea. Electronic address: cklee@hanyang.ac.kr.
authors.forename: Chankil
authors.initials: C
authors.lastname: Lee
authors.affiliation: Department of Bionano Technology, Hanyang University, Ansan 426-791, South Korea. Electronic address: jbchoo@hanyang.ac.kr.
authors.forename: Jaebum
authors.initials: J
authors.lastname: Choo
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry* / Q000235:genetics; D001559:Benzhydryl Compounds / Q000032:analysis*; D060766:Drinking Water / Q000737:chemistry*; D004784:Environmental Monitoring / Q000295:instrumentation; D004867:Equipment Design; D019544:Equipment Failure Analysis; D053768:Metal Nanoparticles / Q000737:chemistry*; D008832:Microchemistry / Q000295:instrumentation; D010636:Phenols / Q000032:analysis*; D013059:Spectrum Analysis, Raman / Q000295:instrumentation*; D020349:Surface Plasmon Resonance / Q000295:instrumentation*; D014874:Water Pollutants, Chemical / Q000032:analysis*; D000098826:Bisphenol A Compounds
chemical_list: D052157:Aptamers, Nucleotide; D001559:Benzhydryl Compounds; D060766:Drinking Water; D010636:Phenols; D014874:Water Pollutants, Chemical; C006780:bisphenol A; D000098826:Bisphenol A Compounds
keywords: Aptamer; Aptasensor; Bisphenol A; Surface-enhanced Raman scattering; Trace analysis