title: Pseudomonas donghuensis sp. nov., exhibiting high-yields of siderophore.
abstract: A strain giving high-yields of siderophores, designated HYS(T), was isolated from the water of East Lake (also called Donghu Lake) of Wuhan in China. Strain HYS(T) is Gram-stain negative, non-spore-forming and rod-shaped with polar flagella. Phylogenetic analysis based on 16S rRNA gene and the other three housekeeping genes (gyrB, rpoD and rpoB) indicated that strain HYS(T) belongs to the genus Pseudomonas. Genomic DNA comparison experiments including DNA-DNA hybridization and whole-genome sequence similarities were performed between HYS(T) and its phylogenetically most closely related type strains, all of the relatedness values are lower than the threshold to ascribe strain HYS(T) to a known species. The major cellular fatty acids of strain HYS(T) are C16:0, C17:0 cyclo, Summed feature 3 (C16:1 ω7c or/and C16:1 ω6c) and Summed feature 8 (C18:1 ω7c or C18:1 ω6c). Its predominant isoprenoid quinone was identified as Q-9, and the minor isoprenoid quinone was Q-8. Phylogenetic analysis together with genomic DNA comparison, phenotypic metabolic tests and chemotaxonomic analysis justified the proposal of strain HYS(T) as a representative of a novel species, for which the name Pseudomonas donghuensis sp. nov. is proposed. The type strain is HYS(T) ( = CCTCC AB 2012141(T) = NRRL B-59108(T)).
authors.affiliation: College of Life Sciences, China Center for Type Culture Collection (CCTCC), Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), State Key Laboratory of Virology, Hubei Provincial Cooperative Innovation Center of Industrial Fermentation, Wuhan University, Wuhan, 430072, People's Republic of China, gjw0039@aliyun.com.
authors.forename: Jingwei
authors.initials: J
authors.lastname: Gao
authors.forename: Guanfang
authors.initials: G
authors.lastname: Xie
authors.forename: Fang
authors.initials: F
authors.lastname: Peng
authors.forename: Zhixiong
authors.initials: Z
authors.lastname: Xie
mesh_terms: D015373:Bacterial Typing Techniques; D002681:China; D016000:Cluster Analysis; D003600:Cytosol / Q000737:chemistry; D027081:DNA Gyrase / Q000235:genetics; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D012321:DNA-Directed RNA Polymerases / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D060106:Lakes / Q000382:microbiology*; D046529:Microscopy, Electron, Transmission; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D011549:Pseudomonas / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D011809:Quinones / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D017262:Siderophores / Q000378:metabolism*; D012808:Sigma Factor / Q000235:genetics
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D011809:Quinones; D012336:RNA, Ribosomal, 16S; D017262:Siderophores; D012808:Sigma Factor; C081775:RNA polymerase sigma 70; D012321:DNA-Directed RNA Polymerases; C105078:RNA polymerase beta subunit; D027081:DNA Gyrase