title: Loss of optineurin in vivo results in elevated cell death and alters axonal trafficking dynamics.
abstract: Mutations in Optineurin have been associated with ALS, glaucoma, and Paget's disease of bone in humans, but little is known about how these mutations contribute to disease. Most of the cellular consequences of Optineurin loss have come from in vitro studies, and it remains unclear whether these same defects would be seen in vivo. To answer this question, we assessed the cellular consequences of Optineurin loss in zebrafish embryos to determine if they showed the same defects as have been described in the in vitro studies. We found that loss of Optineurin resulted in increased cell death, as well as subtle cell morphology, cell migration and vesicle trafficking defects. However, unlike experiments on cells in culture, we found no indication that the Golgi apparatus was disrupted or that NF-κB target genes were upregulated. Therefore, we conclude that in vivo loss of Optineurin shows some, but not all, of the defects seen in in vitro work.
authors.affiliation: Department of Cell Biology, Neurobiology and Anatomy, Medical College of Wisconsin, Milwaukee, WI, United States of America.
authors.forename: Jeremiah D
authors.initials: JD
authors.lastname: Paulus
authors.affiliation: Department of Cell Biology, Neurobiology and Anatomy, Medical College of Wisconsin, Milwaukee, WI, United States of America.
authors.forename: Brian A
authors.initials: BA
authors.lastname: Link
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D017209:Apoptosis; D001369:Axons / Q000378:metabolism*; D001692:Biological Transport / Q000235:genetics; D018797:Cell Cycle Proteins; D002465:Cell Movement; D017124:Conserved Sequence; D004625:Embryo, Nonmammalian / Q000378:metabolism; D005136:Eye Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D055785:Gene Knockdown Techniques; D006801:Humans; D026901:Membrane Transport Proteins; D051379:Mice; D008969:Molecular Sequence Data; D009154:Mutation; D009432:Neural Crest / Q000166:cytology; D017434:Protein Structure, Tertiary; D035582:Transcription Factor TFIIIA / Q000737:chemistry / Q000172:deficiency* / Q000235:genetics* / Q000378:metabolism; D015027:Zebrafish / Q000196:embryology / Q000235:genetics
chemical_list: D018797:Cell Cycle Proteins; D005136:Eye Proteins; D026901:Membrane Transport Proteins; C409460:OPTN protein, human; C501288:Optn protein, mouse; D035582:Transcription Factor TFIIIA