title: Ala307Thr and Asn680Ser polymorphisms of FSHR gene in human reproduction outcomes.
abstract: OBJECTIVE
  It is known that some markers of ovarian stimulation can help to personalize the treatment, adjusting the dose of exogenous rFSH, thus preventing excessive wear of the patient. We aimed to evaluate Ala307Thr and Asn680Ser genotypes of the FSHR gene in infertile women and correlate the findings with the results of ovarian response and assisted reproduction outcomes.
  
  
  METHODS
  Cross-sectional study covering 149 infertile women submitted to assisted reproduction treatment. Genotyping of FSHR variants were performed using TaqMan methodology by real time PCR. FSH and estradiol were measured by ELFA. The data was analyzed statistically.
  
  
  RESULTS
  The frequencies of the FSHR Ala307Thr and Asn680Ser genotypes considering the ovarian hyper stimulation response also did not differ statistically. Considering assisted reproduction outcomes, we observed that the polymorphism Ala307Thr have a statistical difference for the number of MII oocytes and embryos (p=0,051 and p=0.037, respectively), which the genotype Ala/Ala showed more embryos. The polymorphisms did not determine the FSH and estradiol serum levels and the ovarian response in the assisted reproduction treatment.
  
  
  CONCLUSIONS
  The polymorphisms Ala307Thr and Asn680Ser did not determine the FSH and estradiol serum levels and the ovarian response in the assisted reproduction treatment. However, we observed that the Ala307Thr may influence the number of embryos produced.
authors.affiliation: Human Reproduction and Genetics Center - Faculdade de Medicina do ABC, Santo André/SP, Brazil.
authors.forename: Camila Martins
authors.initials: CM
authors.lastname: Trevisan
authors.forename: Carla
authors.initials: C
authors.lastname: Peluso
authors.forename: Emerson Barchi
authors.initials: EB
authors.lastname: Cordts
authors.forename: Renato
authors.initials: R
authors.lastname: de Oliveira
authors.forename: Denise Maria
authors.initials: DM
authors.lastname: Christofolini
authors.forename: Caio Parente
authors.initials: CP
authors.lastname: Barbosa
authors.forename: Bianca
authors.initials: B
authors.lastname: Bianco
mesh_terms: D000328:Adult; D003430:Cross-Sectional Studies; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods; D005787:Gene Frequency / Q000235:genetics; D005838:Genotype; D006801:Humans; D007247:Infertility, Female / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D011446:Prospective Studies; D011962:Receptors, FSH / Q000235:genetics*; D012098:Reproduction / Q000235:genetics*
chemical_list: D011962:Receptors, FSH