title: F2-isoprostanes are correlated with trans fatty acids in the plasma of pregnant women.
abstract: We hypothesized that the mild physiological oxidative stress present during pregnancy could increase both, plasma F2-isoprostanes (F2-isoPs) by lipid oxidation and trans fatty acids (TFA) through cis-trans isomerization respectively. Plasma samples collected at 12-18 weeks (MIROS cohort; n=65) and 38-41 weeks of pregnancy (CHUL cohort; n=21) were subjected to alkaline hydrolysis followed by liquid-liquid extraction in order to extract total F2-isoPs for quantification by HPLC-MS/MS. Several positive correlations were found between F2-isoPs and TFA, measured by GC-FID in plasma phospholipids, such as 6t-18:1, 9t-18:1 and 9t,12c-18:2 (r>0.306; p<0.045). Despite its low level, the 9t,12c-18:2 trans isomer, known to be associated to cardiovascular diseases, showed the most significant correlations with F2-isoPs. No correlation was observed between F2-isoPs and 9t-16:1 or 11t-18:1. In summary, this study suggests either a concomitant phenomenon or a competition between lipid peroxidation and cis-trans isomerisation of the cis precursor fatty acid in vivo during pregnancy.
authors.affiliation: Axe reproduction, santé de la mère et de l׳enfant, CRCHU de Québec, Québec, Canada.
authors.forename: Jessica
authors.initials: J
authors.lastname: Larose
authors.affiliation: Axe endocrinologie et néphrologie, CRCHU de Québec, Québec, Canada; Centre de Recherche en endocrinologie moléculaire et oncologique et génomique humaine (CREMOGH), Université Laval, Québec, Canada; Département de Médecine, Faculté de médecine, Université Laval, Québec, Canada.
authors.forename: Pierre
authors.initials: P
authors.lastname: Julien
authors.affiliation: Axe endocrinologie et néphrologie, CRCHU de Québec, Québec, Canada; Centre de Recherche en endocrinologie moléculaire et oncologique et génomique humaine (CREMOGH), Université Laval, Québec, Canada.
authors.forename: Karine
authors.initials: K
authors.lastname: Greffard
authors.affiliation: Department of Obstetrics and Gynecology, CHU Sainte-Justine and University of Montreal, Montreal, Quebec, Canada.
authors.forename: William D
authors.initials: WD
authors.lastname: Fraser
authors.affiliation: Department of Obstetrics and Gynecology, CHU Sainte-Justine and University of Montreal, Montreal, Quebec, Canada.
authors.forename: Francois
authors.initials: F
authors.lastname: Audibert
authors.affiliation: Department of Obstetrics and Gynecology, CHU Sainte-Justine and University of Montreal, Montreal, Quebec, Canada.
authors.forename: Shu Qin
authors.initials: SQ
authors.lastname: Wei
authors.affiliation: Axe reproduction, santé de la mère et de l׳enfant, CRCHU de Québec, Québec, Canada; Département d׳Obstétrique, Gynécologie et Reproduction, Faculté de médecine, Université Laval, Québec, Canada. Electronic address: jean-francois.bilodeau@crchudequebec.ulaval.ca.
authors.forename: Jean-François
authors.initials: JF
authors.lastname: Bilodeau
mesh_terms: D000293:Adolescent; D000328:Adult; D002851:Chromatography, High Pressure Liquid; D028441:F2-Isoprostanes / Q000097:blood*; D005260:Female; D005865:Gestational Age; D006801:Humans; D006868:Hydrolysis; D007536:Isomerism; D015227:Lipid Peroxidation; D059625:Liquid-Liquid Extraction; D018384:Oxidative Stress; D011247:Pregnancy; D053719:Tandem Mass Spectrometry; D044242:Trans Fatty Acids / Q000097:blood*
chemical_list: D028441:F2-Isoprostanes; D044242:Trans Fatty Acids
keywords: 8-iso-PGF(2α); Elaidic acid; Mass spectrometry; Oxidative stress; Pregnancy; Vaccenic acid