title: Selenium as a versatile center in fluorescence probe for the redox cycle between HClO oxidative stress and H2S repair.
abstract: Selenium is a biologically important trace element and acts as an active center of glutathione peroxidase (GPx). GPx is the important antioxidant enzyme to protect organisms from oxidative damage via catalyzing the reaction between ROS and glutathione (GSH). Mimicking the oxidation-reduction cycles of the versatile selenium core in GPx, we can develop fluorescence probes to detect oxidation and reduction events in living systems. The cellular redox balance between hypochloric acid (HClO) and hydrogen sulfide (H2S) has broad implications in human health and diseases, such as Alzheimer's disease (AD). Therefore, to further investigate the roles of this redox balance and understand the pathogenesis of neurodegenerative diseases, it is necessary to detect the redox state between HClO and H2S in real time. We have developed a reversible fluorescence probe MPhSe-BOD for imaging of the redox cycle between HClO and H2S based on oxidation and reduction of selenide in living cells.
authors.affiliation: Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 457 Zhongshan Road, Shahekou, Dalian, 116023, Liaoning, China.
authors.forename: Zhangrong
authors.initials: Z
authors.lastname: Lou
authors.forename: Peng
authors.initials: P
authors.lastname: Li
authors.forename: Keli
authors.initials: K
authors.lastname: Han
mesh_terms: D065966:Absorption, Physicochemical; D000143:Acids / Q000737:chemistry*; D000818:Animals; D001555:Benzene Derivatives / Q000737:chemistry; D002460:Cell Line; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D006862:Hydrogen Sulfide / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D006997:Hypochlorous Acid / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D018613:Microscopy, Confocal; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D017382:Reactive Oxygen Species / Q000737:chemistry; D019163:Reducing Agents / Q000494:pharmacology; D012643:Selenium / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013997:Time Factors
chemical_list: D000143:Acids; D001555:Benzene Derivatives; D005456:Fluorescent Dyes; D017382:Reactive Oxygen Species; D019163:Reducing Agents; D006997:Hypochlorous Acid; D012643:Selenium; D006862:Hydrogen Sulfide