title: Production of anti-cancer agent using microbial biotransformation.
abstract: Microbial biotransformation is a great model system to produce drugs and biologically active compounds. In this study, we elucidated the fermentation and production of an anti-cancer agent from a microbial process for regiospecific hydroxylation of resveratrol. Among the strains examined, a potent strain showed high regiospecific hydroxylation activity to produce piceatannol. In a 5 L (w/v 3 L) jar fermentation, this wild type Streptomyces sp. in the batch system produced 205 mg of piceatannol (i.e., 60% yields) from 342 mg of resveratrol in 20 h. Using the product, an in vitro anti-cancer study was performed against a human cancer cell line (HeLa). It showed that the biotransformed piceatannol possessed a significant anticancer activity. This result demonstrates that a biotransformation screening method might be of therapeutic interest with respect to the identification of anti-cancer drugs.
authors.affiliation: Division of Biotechnology, Advanced Radiation Technology Institute (ARTI), Korea Atomic Energy Research Institute (KAERI), 1266, Sinjeong-dong, Jeongeup, Jeonbuk 580-185, Korea. chroh@kaeri.re.kr.
authors.forename: Changhyun
authors.initials: C
authors.lastname: Roh
authors.affiliation: Ministry of Environment, Daegu Regional Environmental Office, Government Complex, Hwaam-ro, Dalseo-Gu, Daegu 704-841, Korea. chemnet75@korea.kr.
authors.forename: ChanKyu
authors.initials: C
authors.lastname: Kang
mesh_terms: D000970:Antineoplastic Agents / Q000378:metabolism*; D001711:Biotransformation; D005285:Fermentation; D008401:Gas Chromatography-Mass Spectrometry; D006367:HeLa Cells; D006801:Humans; D006900:Hydroxylation; D009369:Neoplasms / Q000188:drug therapy* / Q000378:metabolism; D000077185:Resveratrol; D013267:Stilbenes / Q000378:metabolism*; D013302:Streptomyces / Q000145:classification / Q000254:growth & development* / Q000378:metabolism
chemical_list: D000970:Antineoplastic Agents; D013267:Stilbenes; C041525:3,3',4,5'-tetrahydroxystilbene; D000077185:Resveratrol