title: PREDICTED STRUCTURE AND BINDING MOTIFS OF COLLAGEN α1(XI).
abstract: The amino propeptide of collagen α1(XI) (NPP) has been shown to bind glycosaminoglycans and to form a dimer. While these are independent biochemical events, it is likely that dimerization facilitates the interaction with glycosaminoglycans or alternatively, that glycosaminoglycan interaction facilitates the formation of an NPP:NPP dimer. The computer program MODELLER was used to generate a homology model of the collagen α1(XI) NPP monomer using the crystal structure of the closely related noncollagenous-4 (NC4) domain of collagen α1(IX) (PDB:2UUR) as the template. Additionally, a dimer model of collagen α1(XI) NPP domain was created based upon the thrombospondin dimer template (PDB:1Z78). The structure of the dimer created in MODELLER was validated by comparison to a dimer model generated by docking two monomers of PDB:2UUR using ClusPro. Calculations of relative binding energy for the interaction between each collagen α1(XI) NPP model and glycosaminoglycans as ligands was performed using AutoDock4. Computational results support a higher affinity between heparan sulfate and a dimer compared to a monomer. These findings are supported by affinity chromatography experiments in which distinct monomer and dimer peaks were observed. Sequential point mutation studies of the putative binding site (147-KKKITK-152) indicated the importance of the basic lysine residue for binding to heparan sulfate. Two orders of magnitude change in binding affinity was predicted when comparing wild type to the mutation K152A. Experimental data supports the predicted change in affinity.
authors.affiliation: Department of Chemistry and Biochemistry, Boise State University, Boise, ID 83725 ; Biomolecular Research Center, Boise State University, Boise, ID 83725.
authors.forename: Owen M
authors.initials: OM
authors.lastname: McDougal
authors.affiliation: Biomolecular Research Center, Boise State University, Boise, ID 83725 ; Department of Materials Science and Engineering, Boise State University, Boise, ID 83725.
authors.forename: Lisa R
authors.initials: LR
authors.lastname: Warner
authors.affiliation: Department of Chemistry and Biochemistry, Boise State University, Boise, ID 83725 ; Biomolecular Research Center, Boise State University, Boise, ID 83725.
authors.forename: Chris
authors.initials: C
authors.lastname: Mallory
authors.affiliation: Biomolecular Research Center, Boise State University, Boise, ID 83725 ; Department of Biological Sciences, Boise State University, Boise, ID 83725.
authors.forename: Julia Thom
authors.initials: JT
authors.lastname: Oxford
keywords: collagen; glycosaminoglycan; heparan sulfate; heparin; molecular interaction; protein