title: Culture of mouse peritoneal macrophages with mouse serum induces lipid bodies that associate with the parasitophorous vacuole and decrease their microbicidal capacity against Toxoplasma gondii.
abstract: Lipid bodies [lipid droplets (LBs)] are lipid-rich organelles involved in lipid metabolism, signalling and inflammation. Recent findings suggest a role for LBs in host response to infection; however, the potential functions of this organelle in Toxoplasma gondii infection and how it alters macrophage microbicidal capacity during infection are not well understood. Here, we investigated the role of host LBs in T. gondii infection in mouse peritoneal macrophages in vitro. Macrophages cultured with mouse serum (MS) had higher numbers of LBs than those cultured in foetal bovine serum and can function as a model to study the role of LBs during intracellular pathogen infection. LBs were found in association with the parasitophorous vacuole, suggesting that T. gondii may benefit from this lipid source. Moreover, increased numbers of macrophage LBs correlated with high prostaglandin E2 (PGE2) production and decreased nitric oxide (NO) synthesis. Accordingly, LB-enriched macrophages cultured with MS were less efficient at controlling T. gondii growth. Treatment of macrophages cultured with MS with indomethacin, an inhibitor of PGE2 production, increased the microbicidal capacity against T. gondii. Collectively, these results suggest that culture with MS caused a decrease in microbicidal activity of macrophages against T. gondii by increasing PGE2 while lowering NO production.
authors.affiliation: Laboratório de Biologia Celular e Tecidual, Universidade Estadual do Norte Fluminense, Campos dos Goytacazes, RJ, Brasil.
authors.forename: Laura Azeredo Miranda
authors.initials: LA
authors.lastname: Mota
authors.affiliation: Laboratório de Biologia Celular e Tecidual, Universidade Estadual do Norte Fluminense, Campos dos Goytacazes, RJ, Brasil.
authors.forename: João
authors.initials: J
authors.lastname: Roberto Neto
authors.affiliation: Laboratório de Biologia Celular e Tecidual, Universidade Estadual do Norte Fluminense, Campos dos Goytacazes, RJ, Brasil.
authors.forename: Verônica Gomes
authors.initials: VG
authors.lastname: Monteiro
authors.affiliation: Laboratório de Biologia Celular e Tecidual, Universidade Estadual do Norte Fluminense, Campos dos Goytacazes, RJ, Brasil.
authors.forename: Caroliny Samary Silva
authors.initials: CS
authors.lastname: Lobato
authors.affiliation: Laboratório de Biologia Celular e Tecidual, Universidade Estadual do Norte Fluminense, Campos dos Goytacazes, RJ, Brasil.
authors.forename: Marco Antonio de
authors.initials: MA
authors.lastname: Oliveira
authors.affiliation: Laboratório de Biologia Celular e Tecidual, Universidade Estadual do Norte Fluminense, Campos dos Goytacazes, RJ, Brasil.
authors.forename: Maura da
authors.initials: Md
authors.lastname: Cunha
authors.affiliation: Laboratório de Imunofarmacologia, Fundação Oswaldo Cruz, Rio de Janeiro, RJ, Brasil.
authors.forename: Heloisa
authors.initials: H
authors.lastname: D'Ávila
authors.affiliation: Laboratório de Tecnologia em Bioquímica e Microscopia, Centro Universitário Estadual da Zona Oeste, Rio de Janeiro, RJ, Brasil.
authors.forename: Sérgio Henrique
authors.initials: SH
authors.lastname: Seabra
authors.affiliation: Laboratório de Imunofarmacologia, Fundação Oswaldo Cruz, Rio de Janeiro, RJ, Brasil.
authors.forename: Patrícia Torres
authors.initials: PT
authors.lastname: Bozza
authors.affiliation: Laboratório de Biologia Celular e Tecidual, Universidade Estadual do Norte Fluminense, Campos dos Goytacazes, RJ, Brasil.
authors.forename: Renato Augusto
authors.initials: RA
authors.lastname: DaMatta
mesh_terms: D000818:Animals; D002417:Cattle; D006790:Host-Parasite Interactions; D007213:Indomethacin / Q000494:pharmacology; D066292:Lipid Droplets / Q000469:parasitology* / Q000502:physiology; D008262:Macrophage Activation / Q000502:physiology*; D017737:Macrophages, Peritoneal / Q000737:chemistry / Q000469:parasitology* / Q000502:physiology / Q000648:ultrastructure; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D009569:Nitric Oxide / Q000096:biosynthesis; D061251:Primary Cell Culture; D011458:Prostaglandins E / Q000037:antagonists & inhibitors / Q000096:biosynthesis; D014122:Toxoplasma / Q000502:physiology*; D014617:Vacuoles / Q000469:parasitology* / Q000502:physiology
chemical_list: D011458:Prostaglandins E; D009569:Nitric Oxide; D007213:Indomethacin