title: Using the Peggy Simple Western system for fine needle aspirate analysis.
abstract: Simple Western™ assays are capillary-based electrophoretic immunoassays, similar in scope to SDS-PAGE (molecular weight separation, "size") and IEF (isoelectric focusing, "charge") immunoblotting. The enhanced sensitivity and automation of the Simple Western makes it better suited to cancer diagnostics and research than the traditional Western platform. Because of its smaller sample volume requirements, primary cells, such as those obtained from fine needle aspirates (FNAs), and solid tumor slices may be used to generate quantitative comparable data. The Peggy™ instrument is capable of performing either size or charge assays on up to 96 samples in a single unattended run.
authors.affiliation: ProteinSimple, 3040 Oakmead Village Dr., Santa Clara, CA, 95051, USA, erik.gentalen@proteinsimple.com.
authors.forename: Erik T
authors.initials: ET
authors.lastname: Gentalen
authors.forename: John M
authors.initials: JM
authors.lastname: Proctor
mesh_terms: D000818:Animals; D001331:Automation; D044963:Biopsy, Fine-Needle; D015153:Blotting, Western / Q000295:instrumentation* / Q000379:methods*; D019075:Electrophoresis, Capillary; D004867:Equipment Design; D005456:Fluorescent Dyes / Q000592:standards; D006801:Humans; D051379:Mice; D009374:Neoplasms, Experimental / Q000473:pathology
chemical_list: D005456:Fluorescent Dyes