title: Transplantation of periaortic adipose tissue from angiotensin receptor blocker-treated mice markedly ameliorates atherosclerosis development in apoE-/- mice.
abstract: BACKGROUND
  Perivascular adipose tissue is implicated in vasoreactivity; however, its effect on atherosclerosis remains undefined.
  
  
  RESULTS
  We examined the effect of a high-cholesterol diet (HCD) on phenotypic alterations of the thoracic periaortic adipose tissue (tPAT) in apoE-deficient (apoE(-/-)) mice. Gene expression of the components of the renin angiotensin system and that of macrophage markers were significantly higher in apoE(-/-) mice fed an HCD than in those fed a chow diet (CD). These changes were absent both in angiotensin II (AngII) receptor blocker (ARB)-treated apoE(-/-) mice and in Ang II type 1 (AT1) receptor-deficient apoE(-/-) (Agtr1(-/-)/apoE(-/-)) mice. To evaluate their effect on atherosclerosis, we transplanted tPAT into apoE(-/-) mice alongside the distal abdominal aorta. Transplanted tPAT was harvested from apoE(-/-) and Agtr1(-/-)/apoE(-/-) mice fed a CD (tPAT-CD/apoE(-/-), tPAT-CD/Agtr1(-/-)/apoE(-/-)), HCD (tPAT-HCD/apoE(-/-), tPAT-HCD/Agtr1(-/-)/apoE(-/-)), or HCD in combination with ARB treatment (tPAT-HCD/ARB/apoE(-/-)). Four weeks after transplantation, a significantly increased oil red O-positive area was observed in the aorta of tPAT-HCD/apoE(-/-) mice than in tPAT-CD/apoE(-/-) mice. Such a change was absent in tPAT-HCD/ARB/apoE(-/-) and tPAT-HCD/Agtr1(-/-)/apoE(-/-) mice.
  
  
  CONCLUSIONS
  Our findings demonstrated that AT1 receptor plays a crucial role in HCD-induced phenotypic alterations of tPAT, modulation of which could exert beneficial effects on atherosclerosis.
authors.affiliation: Department of Cardiovascular Medicine, Kyoto Prefectural University of Medicine, Japan.
authors.forename: Daisuke
authors.initials: D
authors.lastname: Irie
authors.affiliation: Department of Cardiovascular Medicine, Kyoto Prefectural University of Medicine, Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Kawahito
authors.affiliation: Department of Cardiovascular Medicine, Kyoto Prefectural University of Medicine, Japan.
authors.forename: Noriyuki
authors.initials: N
authors.lastname: Wakana
authors.affiliation: Department of Cardiovascular Medicine, Kyoto Prefectural University of Medicine, Japan.
authors.forename: Taku
authors.initials: T
authors.lastname: Kato
authors.affiliation: Department of Cardiovascular Medicine, Kyoto Prefectural University of Medicine, Japan.
authors.forename: Sou
authors.initials: S
authors.lastname: Kishida
authors.affiliation: Department of Cardiovascular Medicine, Kyoto Prefectural University of Medicine, Japan.
authors.forename: Masakazu
authors.initials: M
authors.lastname: Kikai
authors.affiliation: Department of Cardiovascular Medicine, Kyoto Prefectural University of Medicine, Japan.
authors.forename: Takehiro
authors.initials: T
authors.lastname: Ogata
authors.affiliation: Department of Cardiovascular Medicine, Kyoto Prefectural University of Medicine, Japan.
authors.forename: Koji
authors.initials: K
authors.lastname: Ikeda
authors.affiliation: Department of Cardiovascular Medicine, Kyoto Prefectural University of Medicine, Japan.
authors.forename: Tomomi
authors.initials: T
authors.lastname: Ueyama
authors.affiliation: Department of Cardiovascular Medicine, Kyoto Prefectural University of Medicine, Japan.
authors.forename: Satoaki
authors.initials: S
authors.lastname: Matoba
authors.affiliation: Department of Cardiovascular Medicine, Kyoto Prefectural University of Medicine, Japan hiyamada@koto.kpu-m.ac.jp.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Yamada
mesh_terms: D054392:Adipokines / Q000378:metabolism; D000273:Adipose Tissue / Q000637:transplantation*; D057912:Angiotensin II Type 2 Receptor Blockers / Q000494:pharmacology*; D000818:Animals; D001011:Aorta / Q000378:metabolism; D001057:Apolipoproteins E / Q000172:deficiency / Q000235:genetics*; D050197:Atherosclerosis / Q000235:genetics* / Q000473:pathology / Q000517:prevention & control*; D006439:Hemodynamics; D007249:Inflammation / Q000517:prevention & control; D008055:Lipids / Q000097:blood; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D044140:Receptor, Angiotensin, Type 1 / Q000172:deficiency / Q000235:genetics
chemical_list: D054392:Adipokines; D057912:Angiotensin II Type 2 Receptor Blockers; D001057:Apolipoproteins E; D008055:Lipids; D044140:Receptor, Angiotensin, Type 1
keywords: Thoracic periaortic adipose tissue; adipokines; atherosclerosis; macrophage; renin angiotensin system