title: Integrated microRNA and mRNA transcriptome sequencing reveals the potential roles of miRNAs in stage I endometrioid endometrial carcinoma.
abstract: Endometrioid endometrial carcinoma (EEC) is the most dominant subtype of endometrial cancer. Aberrant transcriptional regulation has been implicated in EEC. Herein, we characterized mRNA and miRNA transcriptomes by RNA sequencing in EEC to investigate potential molecular mechanisms underlying the pathogenesis. Total mRNA and small RNA were simultaneously sequenced by next generation sequencing technology for 3 pairs of stage I EEC and adjacent non-tumorous tissues. On average, 52,716,765 pair-end 100 bp mRNA reads and 1,669,602 single-end 50 bp miRNA reads were generated. Further analysis indicated that 7 miRNAs and 320 corresponding target genes were differentially expressed in the three stage I EEC patients. Six of all the seven differentially expressed miRNAs were targeting on eleven differentially expressed genes in the cell cycle pathway. Real-time quantitative PCR in sequencing samples and other independent 21 pairs of samples validated the miRNA-mRNA differential co-expression, which were involved in cell cycle pathway, in the stage I EEC. Thus, we confirmed the involvement of hsa-let-7c-5p and hsa-miR-99a-3p in EEC and firstly found dysregulation of hsa-miR-196a-5p, hsa-miR-328-3p, hsa-miR-337-3p, and hsa-miR-181c-3p in EEC. Moreover, synergistic regulations among these miRNAs were detected. Transcript sequence variants such as single nucleotide variant (SNV) and short insertions and deletions (Indels) were also characterized. Our results provide insights on dysregulated miRNA-mRNA co-expression and valuable resources on transcript variation in stage I EEC, which implies the new molecular mechanisms that underlying pathogenesis of stage I EEC and supplies opportunity for further in depth investigations.
authors.affiliation: Department of Pathology, The Third Affiliated Hospital, Guangzhou Medical University, Guangzhou, China.
authors.forename: Hanzhen
authors.initials: H
authors.lastname: Xiong
authors.affiliation: Department of Pathology, The Third Affiliated Hospital, Guangzhou Medical University, Guangzhou, China.
authors.forename: Qiulian
authors.initials: Q
authors.lastname: Li
authors.affiliation: Department of Pathology, The Third Affiliated Hospital, Guangzhou Medical University, Guangzhou, China.
authors.forename: Shaoyan
authors.initials: S
authors.lastname: Liu
authors.affiliation: Key Laboratory of Major Obstetrics Diseases of Guangdong Province, The Third Affiliated Hospital, Guangzhou Medical University, Guangzhou, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Wang
authors.affiliation: Department of Pathology, The Third Affiliated Hospital, Guangzhou Medical University, Guangzhou, China.
authors.forename: Zhongtang
authors.initials: Z
authors.lastname: Xiong
authors.affiliation: Department of Pathology, The Third Affiliated Hospital, Guangzhou Medical University, Guangzhou, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Chen
authors.affiliation: Department of Pathology, The Third Affiliated Hospital, Guangzhou Medical University, Guangzhou, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Chen
authors.affiliation: Department of Pathology, The Third Affiliated Hospital, Guangzhou Medical University, Guangzhou, China.
authors.forename: Yuexin
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Department of Pathology, The Third Affiliated Hospital, Guangzhou Medical University, Guangzhou, China.
authors.forename: Xuexian
authors.initials: X
authors.lastname: Tan
authors.affiliation: Department of Pathology, The Third Affiliated Hospital, Guangzhou Medical University, Guangzhou, China.
authors.forename: Qiuping
authors.initials: Q
authors.lastname: Luo
authors.affiliation: Department of Pathology, The Third Affiliated Hospital, Guangzhou Medical University, Guangzhou, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Peng
authors.affiliation: Key Laboratory of Major Obstetrics Diseases of Guangdong Province, The Third Affiliated Hospital, Guangzhou Medical University, Guangzhou, China.
authors.forename: Guohong
authors.initials: G
authors.lastname: Xiao
authors.affiliation: Department of Pathology, The Third Affiliated Hospital, Guangzhou Medical University, Guangzhou, China; Key Laboratory of Major Obstetrics Diseases of Guangdong Province, The Third Affiliated Hospital, Guangzhou Medical University, Guangzhou, China.
authors.forename: Qingping
authors.initials: Q
authors.lastname: Jiang
mesh_terms: D000328:Adult; D018269:Carcinoma, Endometrioid / Q000175:diagnosis / Q000235:genetics* / Q000378:metabolism; D016889:Endometrial Neoplasms / Q000175:diagnosis / Q000235:genetics* / Q000378:metabolism; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D059014:High-Throughput Nucleotide Sequencing; D006801:Humans; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D008875:Middle Aged; D017423:Sequence Analysis, RNA; D059467:Transcriptome
chemical_list: D035683:MicroRNAs