title: Identification of bacteria in water by a fluorescent array.
abstract: We report a method for the rapid and efficient identification of bacteria making use of five probes having fluorescent characteristics (F-array) and subsequent statistical analysis. Eight kinds of bacteria, including normal and multidrug-resistant bacteria, are differentiated successfully. Our easy-to-perform and time-saving method consists of mixing bacteria and probes, recording fluorescent intensity data by automated flow cytometry, and statistical analysis. No washing steps are required in order to identify the different bacteria simultaneously.
authors.affiliation: Beijing Engineering Research Center for BioNanotechnology & Key Laboratory for Biological Effects of Nanomaterials and Nanosafety, National Center for NanoScience and Technology, 11 Beiyitiao, ZhongGuanCun, Beijing 100190 (China); University of Chinese Academy of Sciences, Beijing 100049 (China).
authors.forename: Wenwen
authors.initials: W
authors.lastname: Chen
authors.forename: Qizhai
authors.initials: Q
authors.lastname: Li
authors.forename: Wenshu
authors.initials: W
authors.lastname: Zheng
authors.forename: Fang
authors.initials: F
authors.lastname: Hu
authors.forename: Guanxin
authors.initials: G
authors.lastname: Zhang
authors.forename: Zhuo
authors.initials: Z
authors.lastname: Wang
authors.forename: Deqing
authors.initials: D
authors.lastname: Zhang
authors.forename: Xingyu
authors.initials: X
authors.lastname: Jiang
mesh_terms: D001419:Bacteria / Q000145:classification / Q000302:isolation & purification*; D005456:Fluorescent Dyes; D025341:Principal Component Analysis; D014871:Water Microbiology
chemical_list: D005456:Fluorescent Dyes
keywords: bacteria; biosensors; fluorescent probes; mathematical analysis