title: Increased Angiogenesis and Decreased Programmed Cell Death Increases the Risk of Uterine Cervical Cancer.
abstract: BACKGROUND
  Cervical cancer is one of the most common female malignancies and leading cause for high mortality rate. In the present study we made an attempt to determine the extent of angiogenesis, apoptosis, accumulation of mutant p53 protein, cell proliferation rate in the uterine cervical cancer tissues.
  
  
  METHODS
  Cervical cancer samples were obtained from patients and they were subjected to PCR analysis and immunocytochemistry.
  
  
  RESULTS
  A total of 30 cervical cancer tissue samples were analyzed, by PCR, we found 25 collected cervical cancer samples showed HPV-16 and E6 positive. Further, we observed the increased CD34 expression was associated with HPV-16 and E6 positive cancer tissues when compared to the corresponding control tissues. This elevated level of CD34 confirms the increased extent of angiogenesis in cervical cancer tissues. Further by immunocytochemistry we have demonstrated that the rate of apoptosis is reduced, over expression of bcl-2, Ki 67 and thus increases rate of cell proliferation.
  
  
  CONCLUSIONS
  Therefore, our data suggest that development of new anticancer or antiviral drugs could efficiently compromise the HPV-16 mediated angiogenesis and reduced apoptosis in cervical cancer and thus will improve the survival rate of patients.
authors.affiliation: Department of Gynaecology, Jinan Maternity and Child Care Hospital, Shandong, China.
authors.forename: Z-l
authors.initials: ZL
authors.lastname: Qin
authors.affiliation: Department of Gynaecology, Jinan Maternity and Child Care Hospital, Shandong, China.
authors.forename: X-x
authors.initials: XX
authors.lastname: Zheng
authors.affiliation: Department of Gynaecology, Jinan Maternity and Child Care Hospital, Shandong, China.
authors.forename: Y-h
authors.initials: YH
authors.lastname: Xu
mesh_terms: D000328:Adult; D018952:Antigens, CD34 / Q000235:genetics; D017209:Apoptosis; D016022:Case-Control Studies; D049109:Cell Proliferation / Q000502:physiology; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D052162:Human papillomavirus 16 / Q000302:isolation & purification; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D019394:Ki-67 Antigen / Q000235:genetics; D009389:Neovascularization, Pathologic / Q000473:pathology*; D016133:Polymerase Chain Reaction; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics; D012306:Risk; D016159:Tumor Suppressor Protein p53 / Q000235:genetics*; D002583:Uterine Cervical Neoplasms / Q000098:blood supply / Q000235:genetics / Q000473:pathology*
chemical_list: D018952:Antigens, CD34; D019394:Ki-67 Antigen; D019253:Proto-Oncogene Proteins c-bcl-2; D016159:Tumor Suppressor Protein p53