title: High-fat diet promotes neuronal loss in the myenteric plexus of the large intestine in mice.
abstract: BACKGROUND
  Obesity is considered a risk factor for other chronic diseases, and diets rich in lipids can cause alterations in the intestinal functions.
  
  
  OBJECTIVE
  The aim of this study was to investigate the effects of a high-fat diet (HFD) on the myenteric plexus of the large intestine in mice.
  
  
  METHODS
  Swiss mice were distributed into four groups: Control animals fed standard chow for 8 and 17 weeks (C8 and C17 groups) and hyperlipidic animals fed HFD for 8 and 17 weeks (Ob8 and Ob17 groups). Immunofluorescence was performed in the large intestine for the morphologic and quantitative analysis of neuronal populations.
  
  
  RESULTS
  Animals in the Ob17 group exhibited increased body weight and visceral fat gain compared with the C17 group. The intestinal area was also reduced in the two Ob groups. In the proximal colon, the Ob17 group exhibited 16.1 % reduction of the general neuronal density and 33 % reduction of the VIP-immunoreactive (IR) subpopulation. The general neuronal density in the distal colon was reduced by 45 % in the Ob17 group, and the nNOS-IR density was reduced by 35 %. The morphometry of neuronal cell bodies in the Ob17 group exhibited a reduction of the neuronal area of all of the neuronal populations studied in the proximal colon, with a reduction of the subpopulations of nNOS-IR and VIP-IR neurons in the distal colon.
  
  
  CONCLUSIONS
  The HFD caused neuronal loss in the myenteric plexus, and nitrergic neurons were more resilient. The changes were more pronounced in the distal colon after 17 weeks.
authors.affiliation: Department of Morphological Sciences, State University of Maringá, Av. Colombo, 5790, Maringá, PR, CEP 87020-900, Brazil, evandroberaldi@gmail.com.
authors.forename: Evandro José
authors.initials: EJ
authors.lastname: Beraldi
authors.forename: Angélica
authors.initials: A
authors.lastname: Soares
authors.forename: Stephanie Carvalho
authors.initials: SC
authors.lastname: Borges
authors.forename: Aline Cristine da Silva
authors.initials: AC
authors.lastname: de Souza
authors.forename: Maria Raquel Marçal
authors.initials: MR
authors.lastname: Natali
authors.forename: Roberto Barbosa
authors.initials: RB
authors.lastname: Bazotte
authors.forename: Nilza Cristina
authors.initials: NC
authors.lastname: Buttow
mesh_terms: D000818:Animals; D003106:Colon / Q000294:innervation*; D059305:Diet, High-Fat / Q000009:adverse effects*; D008297:Male; D051379:Mice; D009197:Myenteric Plexus; D026602:Nitrergic Neurons; D014660:Vasoactive Intestinal Peptide
chemical_list: D014660:Vasoactive Intestinal Peptide