title: Inhibition of protein phosphatase 5 (PP5) suppresses survival and growth of colorectal cancer cells.
abstract: Protein phosphatase 5 (PP5) is a unique member of the protein phosphatases family that functions in multiple signaling pathways involved in DNA damage, cell cycle control, cell growth, and apoptosis. Recent evidence indicated that PP5 may play a role in cancer progression. In this study, we aimed to examine the biological effect of PP5 on cell growth and apoptosis in human colorectal cancer (CRC). We first knocked down PP5 expression in RKO cells via a short hairpin RNA containing lentivirus system. Then, methylthiazoletetrazolium assay, colony formation assay, and flow cytometry analysis were performed. The proliferation and colony formation ability of RKO cells were remarkably suppressed in PP5-silenced groups, as compared with control groups. Moreover, downregulation of PP5 resulted in a significant G0/G1 phase cell cycle arrest and an induction of apoptosis. In all, these results demonstrated the importance of PP5 in CRC cell growth, and it might be used as a potential therapeutic target for the treatment of CRC.
authors.affiliation: Department of Surgical Oncology, Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, People's Republic of China.
authors.forename: Jianwei
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Surgical Oncology, Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, People's Republic of China.
authors.forename: Jinhui
authors.initials: J
authors.lastname: Zhu
authors.affiliation: Department of Anorectal Surgery, Ningbo Second Hospital, Ningbo, People's Republic of China.
authors.forename: Mingjun
authors.initials: M
authors.lastname: Dong
authors.affiliation: Department of Anorectal Surgery, Ningbo Second Hospital, Ningbo, People's Republic of China.
authors.forename: Hua
authors.initials: H
authors.lastname: Yu
authors.affiliation: Department of Anorectal Surgery, Ningbo Second Hospital, Ningbo, People's Republic of China.
authors.forename: Xiaoyu
authors.initials: X
authors.lastname: Dai
authors.affiliation: Clinical Research Center, Ningbo Second Hospital, Key Laboratory of Molecular Biology of Cancer, Ningbo, People's Republic of China.
authors.forename: Keqiang
authors.initials: K
authors.lastname: Li
mesh_terms: D017209:Apoptosis / Q000235:genetics; D002453:Cell Cycle / Q000235:genetics; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics; D002470:Cell Survival / Q000235:genetics; D015179:Colorectal Neoplasms / Q000473:pathology*; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics; D055785:Gene Knockdown Techniques; D020868:Gene Silencing; D006801:Humans; D016086:Lentivirus / Q000235:genetics; D009687:Nuclear Proteins / Q000172:deficiency* / Q000235:genetics*; D010749:Phosphoprotein Phosphatases / Q000172:deficiency* / Q000235:genetics*; D034741:RNA, Small Interfering / Q000235:genetics
chemical_list: D009687:Nuclear Proteins; D034741:RNA, Small Interfering; D010749:Phosphoprotein Phosphatases; C089641:protein phosphatase 5
keywords: apoptosis; cell cycle; colorectal cancer; protein phosphatase 5; shRNA