title: Quantification of amikacin and kanamycin in serum using a simple and validated LC-MS/MS method.
abstract: BACKGROUND
  Amikacin and kanamycin are frequently used in the treatment of multidrug-resistant TB. The current commercially available immunoassay is unable to analyze kanamycin and trough levels of amikacin. The objective was therefore to develop a LC-MS/MS method for the quantification of amikacin and kanamycin in human serum.
  
  
  METHODS
  Using apramycin as internal standard, selectivity, accuracy, precision, recovery, matrix effects and stability were evaluated.
  
  
  RESULTS
  The presented LC-MS/MS method meets the recommendations of the US FDA with a low LLOQ of 250 ng/ml for amikacin and 100 ng/ml for kanamycin. No statistical significant difference was found between the LC-MS/MS method and the immunoassay of amikacin (Architect(®) assay, p = 0.501).
  
  
  CONCLUSIONS
  The low LLOQ of amikacin and the ability to analyze kanamycin makes the LC-MS/MS method the preferred method for analyzing these aminoglycosides.
authors.affiliation: University of Groningen, University Medical Center Groningen, Department of Clinical Pharmacy & Pharmacology, PO Box 30.001, 9700 RB Groningen, The Netherlands.
authors.forename: Jacob A
authors.initials: JA
authors.lastname: Dijkstra
authors.forename: Marieke Gg
authors.initials: MG
authors.lastname: Sturkenboom
authors.forename: Kai van
authors.initials: Kv
authors.lastname: Hateren
authors.forename: Remco A
authors.initials: RA
authors.lastname: Koster
authors.forename: Ben
authors.initials: B
authors.lastname: Greijdanus
authors.forename: Jan-Willem C
authors.initials: JW
authors.lastname: Alffenaar
mesh_terms: D000328:Adult; D000368:Aged; D000583:Amikacin / Q000097:blood*; D000900:Anti-Bacterial Agents / Q000097:blood*; D002853:Chromatography, Liquid / Q000379:methods; D016903:Drug Monitoring / Q000379:methods*; D005260:Female; D006801:Humans; D007612:Kanamycin / Q000097:blood*; D008297:Male; D008875:Middle Aged; D053719:Tandem Mass Spectrometry / Q000379:methods; D055815:Young Adult
chemical_list: D000900:Anti-Bacterial Agents; D007612:Kanamycin; D000583:Amikacin