title: Surface glycosaminoglycans protect eukaryotic cells against membrane-driven peptide bacteriocins.
abstract: Enzymatic elimination of surface glycosaminoglycans or inhibition of their sulfation provokes sensitizing of HT-29 and HeLa cells toward the peptide bacteriocins nisin A, plantaricin C, and pediocin PA-1/AcH. The effect can be partially reversed by heparin, which also lowers the susceptibility of Lactococcus lactis to nisin A. These data indicate that the negative charge of the glycosaminoglycan sulfate residues binds the positively charged bacteriocins, thus protecting eukaryotic cells from plasma membrane damage.
authors.affiliation: Área de Microbiología, Universidad de Oviedo, Oviedo, Spain Instituto Universitario de Biotecnología, Universidad de Oviedo, Oviedo, Spain.
authors.forename: Rebeca
authors.initials: R
authors.lastname: Martín
authors.affiliation: Área de Microbiología, Universidad de Oviedo, Oviedo, Spain Instituto Universitario de Biotecnología, Universidad de Oviedo, Oviedo, Spain.
authors.forename: Susana
authors.initials: S
authors.lastname: Escobedo
authors.affiliation: Área de Microbiología, Universidad de Oviedo, Oviedo, Spain Instituto Universitario de Biotecnología, Universidad de Oviedo, Oviedo, Spain.
authors.forename: Carla
authors.initials: C
authors.lastname: Martín
authors.affiliation: Área de Microbiología, Universidad de Oviedo, Oviedo, Spain Departamento de Biotecnología de Neiker-Tecnalia Arkaute, Vitoria-Gasteiz, Spain.
authors.forename: Ainara
authors.initials: A
authors.lastname: Crespo
authors.affiliation: Área de Microbiología, Universidad de Oviedo, Oviedo, Spain Instituto Universitario de Oncología del Principado de Asturias (IUOPA), Universidad de Oviedo, Oviedo, Spain.
authors.forename: Luis M
authors.initials: LM
authors.lastname: Quiros
authors.affiliation: Área de Microbiología, Universidad de Oviedo, Oviedo, Spain Instituto Universitario de Biotecnología, Universidad de Oviedo, Oviedo, Spain Instituto de Productos Lácteos de Asturias (IPLA-CSIC), Villaviciosa, Spain evaristo@uniovi.es.
authors.forename: Juan E
authors.initials: JE
authors.lastname: Suarez
mesh_terms: D001430:Bacteriocins / Q000494:pharmacology*; D002462:Cell Membrane / Q000187:drug effects / Q000502:physiology; D006025:Glycosaminoglycans / Q000502:physiology*; D019073:HT29 Cells / Q000187:drug effects / Q000502:physiology; D006367:HeLa Cells / Q000187:drug effects / Q000502:physiology; D006493:Heparin / Q000494:pharmacology; D006801:Humans; D013294:Lactococcus lactis / Q000378:metabolism; D009561:Nisin / Q000494:pharmacology; D000070017:Pediocins
chemical_list: D001430:Bacteriocins; D006025:Glycosaminoglycans; D000070017:Pediocins; C089260:plantaricin C; C074288:pediocin PA-1; D009561:Nisin; D006493:Heparin; C102526:nisin A