title: Oenococcus alcoholitolerans sp. nov., a lactic acid bacteria isolated from cachaça and ethanol fermentation processes.
abstract: Four strains of lactic acid bacteria isolated from cachaça and alcohol fermentation vats in Brazil were characterised in order to determine their taxonomic position. Phylogenetic analysis revealed that they belong to the genus Oenococcus and should be distinguished from their closest neighbours. The 16S rRNA gene sequence similarity against the type strains of the other two species of the genus was below 94.76 % (Oenococcus kitaharae) and 94.62 % (Oenococcus oeni). The phylogeny based on pheS gene sequences also confirmed the position of the new taxon. DNA-DNA hybridizations based on in silico genome-to-genome comparison, Average Amino Acid Identity, Average Nucleotide Identity and Karlin genomic signature confirmed the novelty of the taxon. Distinctive phenotypic characteristics are the ability to metabolise sucrose but not trehalose. The name Oenococcus alcoholitolerans sp. nov. is proposed for this taxon, with the type strain UFRJ-M7.2.18(T) ( = CBAS474(T) = LMG27599(T)). In addition, we have determined a draft genome sequence of the type strain.
authors.affiliation: Departamento de Microbiologia, Instituto de Ciências Biológicas (ICB), Universidade Federal de Minas Gerais, C.P. 486, Belo Horizonte, MG, 31270-901, Brazil, fbadotti@cebio.org.
authors.forename: Fernanda
authors.initials: F
authors.lastname: Badotti
authors.forename: Ana Paula B
authors.initials: AP
authors.lastname: Moreira
authors.forename: Luciane A Chimetto
authors.initials: LA
authors.lastname: Tonon
authors.forename: Brígida T Luckwu
authors.initials: BT
authors.lastname: de Lucena
authors.forename: Fátima de Cássia O
authors.initials: Fde C
authors.lastname: Gomes
authors.forename: Ricardo
authors.initials: R
authors.lastname: Kruger
authors.forename: Cristiane C
authors.initials: CC
authors.lastname: Thompson
authors.forename: Marcos Antonio
authors.initials: MA
authors.lastname: de Morais
authors.forename: Carlos A
authors.initials: CA
authors.lastname: Rosa
authors.forename: Fabiano L
authors.initials: FL
authors.lastname: Thompson
mesh_terms: D015373:Bacterial Typing Techniques; D001938:Brazil; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation; D005516:Food Microbiology; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D056585:Oenococcus / Q000145:classification* / Q000302:isolation & purification*; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D000431:Ethanol