title: Synergism in the effect of prior jasmonic acid application on herbivore-induced volatile emission by Lima bean plants: transcription of a monoterpene synthase gene and volatile emission.
abstract: Jasmonic acid (JA) plays a central role in induced plant defence e.g. by regulating the biosynthesis of herbivore-induced plant volatiles that mediate the attraction of natural enemies of herbivores. Moreover, exogenous application of JA can be used to elicit plant defence responses similar to those induced by biting-chewing herbivores and mites that pierce cells and consume their contents. In the present study, we used Lima bean (Phaseolus lunatus) plants to explore how application of a low dose of JA followed by minor herbivory by spider mites (Tetranychus urticae) affects transcript levels of P. lunatus (E)-β-ocimene synthase (PlOS), emission of (E)-β-ocimene and nine other plant volatiles commonly associated with herbivory. Furthermore, we investigated the plant's phytohormonal response. Application of a low dose of JA increased PlOS transcript levels in a synergistic manner when followed by minor herbivory for both simultaneous and sequential infestation. Emission of (E)-β-ocimene was also increased, and only JA, but not SA, levels were affected by treatments. Projection to latent structures-discriminant analysis (PLS-DA) of other volatiles showed overlap between treatments. Thus, a low-dose JA application results in a synergistic effect on gene transcription and an increased emission of a volatile compound involved in indirect defence after herbivore infestation.
authors.affiliation: Laboratory of Entomology, Wageningen University, P.O. Box 8031, 6700 EH Wageningen, The Netherlands.
authors.forename: Tila R
authors.initials: TR
authors.lastname: Menzel
authors.affiliation: Laboratory of Entomology, Wageningen University, P.O. Box 8031, 6700 EH Wageningen, The Netherlands.
authors.forename: Berhane T
authors.initials: BT
authors.lastname: Weldegergis
authors.affiliation: Max Planck Institute for Chemical Ecology, Hans Knoell Strasse 8, D-07745 Jena, Germany.
authors.forename: Anja
authors.initials: A
authors.lastname: David
authors.affiliation: Max Planck Institute for Chemical Ecology, Hans Knoell Strasse 8, D-07745 Jena, Germany.
authors.forename: Wilhelm
authors.initials: W
authors.lastname: Boland
authors.affiliation: Laboratory of Entomology, Wageningen University, P.O. Box 8031, 6700 EH Wageningen, The Netherlands.
authors.forename: Rieta
authors.initials: R
authors.lastname: Gols
authors.affiliation: Laboratory of Entomology, Wageningen University, P.O. Box 8031, 6700 EH Wageningen, The Netherlands.
authors.forename: Joop J A
authors.initials: JJ
authors.lastname: van Loon
authors.affiliation: Laboratory of Entomology, Wageningen University, P.O. Box 8031, 6700 EH Wageningen, The Netherlands marcel.dicke@wur.nl.
authors.forename: Marcel
authors.initials: M
authors.lastname: Dicke
mesh_terms: D000080462:Acyclic Monoterpenes; D000475:Alkenes / Q000378:metabolism; D000818:Animals; D003517:Cyclopentanes / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D018506:Gene Expression Regulation, Plant / Q000187:drug effects*; D060434:Herbivory; D019753:Intramolecular Lyases / Q000235:genetics* / Q000378:metabolism; D054883:Oxylipins / Q000494:pharmacology*; D027805:Phaseolus / Q000235:genetics* / Q000378:metabolism; D010937:Plant Growth Regulators / Q000494:pharmacology*; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D016133:Polymerase Chain Reaction; D013729:Terpenes / Q000378:metabolism; D040102:Tetranychidae / Q000502:physiology
chemical_list: D000080462:Acyclic Monoterpenes; D000475:Alkenes; D003517:Cyclopentanes; D054883:Oxylipins; D010937:Plant Growth Regulators; D010940:Plant Proteins; D013729:Terpenes; C443996:beta-ocimene; C011006:jasmonic acid; D019753:Intramolecular Lyases; C040265:pinene cyclase I
keywords: Herbivore-induced plant volatiles; induced plant defence; mite–plant interactions; phytohormones; plant memory; priming.; terpene synthase