title: The ∼ 16 kDa C-terminal sequence of clathrin assembly protein AP180 is essential for efficient clathrin binding.
abstract: Brain-specific AP180 is present in clathrin coats at equal concentration to the adapter complex, AP2, and assembles clathrin faster than any other protein in vitro. Both AP180 and its ubiquitously expressed homolog clathrin assembly lymphoid myeloid leukemia protein (CALM) control vesicle size and shape in clathrin mediated endocytosis. The clathrin assembly role of AP180 is mediated by a long disordered C-terminal assembly domain. Within this assembly domain, a central acidic clathrin and adapter binding (CLAP) sub-domain contains all of the known short binding motifs for clathrin and AP2. The role of the remaining ∼ 16 kDa C-terminal sequence has not been clear. We show that this sequence has a separate function in ensuring efficient binding of clathrin, based on in vitro binding and ex vivo transferrin uptake assays. Sequence alignment suggests the C-terminal sub-domain is conserved in CALM.
authors.affiliation: Children's Medical Research Institute, The University of Sydney, Westmead, New South Wales, Australia.
authors.forename: Ling-Shan
authors.initials: LS
authors.lastname: Chan
authors.affiliation: Children's Medical Research Institute, The University of Sydney, Westmead, New South Wales, Australia.
authors.forename: Lia
authors.initials: L
authors.lastname: Moshkanbaryans
authors.affiliation: Children's Medical Research Institute, The University of Sydney, Westmead, New South Wales, Australia.
authors.forename: Jing
authors.initials: J
authors.lastname: Xue
authors.affiliation: Children's Medical Research Institute, The University of Sydney, Westmead, New South Wales, Australia.
authors.forename: Mark E
authors.initials: ME
authors.lastname: Graham
mesh_terms: D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D000818:Animals; D019556:COS Cells; D002522:Chlorocebus aethiops; D002966:Clathrin / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D051379:Mice; D034041:Monomeric Clathrin Assembly Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D011485:Protein Binding; D017434:Protein Structure, Tertiary
chemical_list: D002966:Clathrin; D034041:Monomeric Clathrin Assembly Proteins; C573094:PICALM protein, mouse; C439023:clathrin assembly protein AP180