title: Evaluation of broad-range 16S rRNA PCR for the diagnosis of bloodstream infections: two years of experience.
abstract: BACKGROUND
  Diagnosis of bloodstream infections using bacteriological cultures suffers from low sensitivity and reporting delay. Advanced molecular techniques introduced in many laboratories provide rapid results and may show improvements in patient outcomes. This study aimed to evaluate the usefulness of a molecular technique, broad-range 16S rRNA PCR followed by sequencing for the diagnosis of bloodstream infections, compared to blood culture in different patient groups.
  
  
  METHODS
  Conventional PCR was performed, using broad-range 16S rRNA primers, on blood cultures collected from different patients with suspected bloodstream infections; results were compared with those of blood culture.
  
  
  RESULTS
  Though blood culture is regarded as the gold standard, PCR evaluation showed sensitivity of 86.25%, specificity of 91.25%, positive predictive value of 76.67%, negative predictive value of 95.22%, and accuracy of 88.8%.
  
  
  CONCLUSIONS
  Molecular assays seem not to be sufficient to replace microbial cultures in the diagnosis of bloodstream infections, but they can offer a rapid, good negative test to rule out infection due to their high negative predictive value.
authors.affiliation: Faculty of Medicine, Cairo University, Cairo, Egypt. reem.mostafa@kasralainy.edu.eg.
authors.forename: Reem Mostafa
authors.initials: RM
authors.lastname: Hassan
authors.forename: Mervat G
authors.initials: MG
authors.lastname: El Enany
authors.forename: Hussien H
authors.initials: HH
authors.lastname: Rizk
mesh_terms: D016470:Bacteremia / Q000175:diagnosis* / Q000382:microbiology*; D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D001431:Bacteriological Techniques / Q000379:methods*; D006801:Humans; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods*; D011237:Predictive Value of Tests; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA / Q000379:methods; D013997:Time Factors
chemical_list: D012336:RNA, Ribosomal, 16S