title: Kenaf biomass biodecomposition by basidiomycetes and actinobacteria in submerged fermentation for production of carbohydrates and phenolic compounds.
abstract: The efficiency and dynamics of simultaneous kenaf biomass decomposition by basidiomycetous fungi and actinobacteria were investigated. After 8weeks of incubation, up to 34wt.% of the kenaf biomass was degraded, with the combination of fungi and bacteria being the most efficient. Lignin decomposition accounted for ∼20% of the observed biomass reduction, regardless of the culture used. The remaining 80% of biomass degradation was due to carbohydrate based polymers. Major monosaccharides were produced in tangible yields (26-38%) at different times. Glucose, fructose and xylose were then fully consumed by day 25 while some galactose persisted until day 45. Once monosaccharides were depleted, the production of laccase, manganese-dependent peroxidase and lignin peroxidase enzymes, essential for lignin decomposition, was induced. The products of lignin biodecomposition were shown to be water-soluble and characterized by thermal desorption-pyrolysis-gas chromatography.
authors.affiliation: Department of Chemical Engineering, University of North Dakota, Grand Forks, ND, USA.
authors.forename: Ivana
authors.initials: I
authors.lastname: Brzonova
authors.affiliation: Department of Chemistry, University of North Dakota, Grand Forks, ND, USA.
authors.forename: Evguenii
authors.initials: E
authors.lastname: Kozliak
authors.affiliation: Department of Chemistry, University of North Dakota, Grand Forks, ND, USA.
authors.forename: Alena
authors.initials: A
authors.lastname: Kubátová
authors.affiliation: Department of Chemical and Material Engineering, California State Polytechnic University, Pomona, CA, USA.
authors.forename: Michelle
authors.initials: M
authors.lastname: Chebeir
authors.affiliation: Department of Biology, Lakehead University, Thunder Bay, ON, Canada.
authors.forename: Wensheng
authors.initials: W
authors.lastname: Qin
authors.affiliation: Center for Bioprocessing Research and Development, South Dakota School of Mines & Technology, Rapid City, SD, USA.
authors.forename: Lew
authors.initials: L
authors.lastname: Christopher
authors.affiliation: Department of Chemical Engineering, University of North Dakota, Grand Forks, ND, USA.
authors.forename: Yun
authors.initials: Y
authors.lastname: Ji
mesh_terms: D039903:Actinobacteria / Q000378:metabolism*; D001487:Basidiomycota / Q000378:metabolism*; D001673:Biodegradation, Environmental; D056804:Biofuels; D018533:Biomass; D050260:Carbohydrate Metabolism; D005285:Fermentation; D031584:Hibiscus / Q000378:metabolism*; D008031:Lignin / Q000378:metabolism; D010636:Phenols / Q000378:metabolism
chemical_list: D056804:Biofuels; D010636:Phenols; D008031:Lignin
keywords: Actinobacteria; Basidiomycetes; Biomass; Kenaf; Lignin