title: Analysis of relationship between oxidized phospholipid structure and interaction with the class B scavenger receptors.
abstract: Recognition of specific oxidized phospholipids oxPCCD36 by scavenger receptors CD36 and SR-BI plays a critical role in several pathophysiological processes. The structural basis for the recognition of oxPCCD36 by CD36 and SR-BI is poorly understood. We describe here the design and synthesis of a series of model oxidized phospholipids having various functional groups at sn-1, sn-2, and sn-3 positions. Synthetic methodologies and experimental details for the preparation of specific examples of model oxidized phospholipids are presented. The correlation between their structure and their ability to serve as ligands for CD36 and SR-BI was determined using competitive binding assay on cells overexpressing scavenger receptors, direct binding assay to scavenger receptors expressed as GST-fusion proteins, and cholesterol ester synthesis assay using mouse peritoneal macrophages.
authors.affiliation: Department of Molecular Cardiology, Lerner Research Institute, Cleveland Clinic, 9500 Euclid Avenue, ND50, Cleveland, OH, 44195, USA.
authors.forename: Detao
authors.initials: D
authors.lastname: Gao
authors.forename: Lawrence M
authors.initials: LM
authors.lastname: Sayre
authors.forename: Eugene A
authors.initials: EA
authors.lastname: Podrez
mesh_terms: D000818:Animals; D001665:Binding Sites; D001667:Binding, Competitive; D001671:Biochemistry / Q000379:methods*; D001681:Biological Assay; D018955:CD36 Antigens / Q000378:metabolism; D002788:Cholesterol Esters / Q000378:metabolism; D005487:Foam Cells / Q000378:metabolism; D057809:HEK293 Cells; D006801:Humans; D051379:Mice; D010084:Oxidation-Reduction; D010743:Phospholipids / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism*; D051122:Scavenger Receptors, Class B / Q000378:metabolism*
chemical_list: D018955:CD36 Antigens; D002788:Cholesterol Esters; D010743:Phospholipids; D051122:Scavenger Receptors, Class B