title: Effect of vitamin C administration on hydrogen peroxide-induced cytotoxicity in periodontal ligament cells.
abstract: Periodontitis is a disease, which is associated with chronic inflammation and leads to significant destruction of periodontal tissues. Periodontal ligament cells (PDLCs) constitute the largest cell population in PDL tissues and a considerable body of evidence has demonstrated an association between oxidative stress and the progression of periodontitis. However, the effects on PDLCs exposed to hydrogen peroxide (H2O2) and the molecular mechanisms by which H2O2 affects periodontitis remain to be elucidated. In the present study, the potential cytotoxic effect of H2O2 and the antioxidative function of vitamin C (Vc) in PDLCs were investigated. The results demonstrated that H2O2 treatment decreased the viability of PDLCs. The decreased PDLC viability was primarily induced by apoptosis, which was evidenced by cleaved caspases-3, caspases-9 and poly (ADP-ribose) polymerase. Following optimal Vc addition, the proapoptotic effects of H2O2 were partially antagonized. Taken together, the present study demonstrated that H2O2 primarily induced the apoptosis of PDLCs and that these adverse effects were partially rescued following treatment with Vc. These results revealed how H2O2 promotes the progression of periodontitis and provide an improved understanding of the reversal effect of antioxidant treatment. Therefore, optimal Vc administration may provide a potentially effective technique in periodontal therapy.
authors.affiliation: Institute and Hospital of Stomatology, Nanjing University Medical School, Nanjing, Jiangsu 210008, P.R. China.
authors.forename: Wenlei
authors.initials: W
authors.lastname: Wu
authors.affiliation: State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing, Jiangsu 210093, P.R. China.
authors.forename: Nanfei
authors.initials: N
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing, Jiangsu 210093, P.R. China.
authors.forename: Xiujing
authors.initials: X
authors.lastname: Feng
authors.affiliation: School of Life Sciences, Anqing Normal University, Anqing, Anhui 246000, P.R. China.
authors.forename: Tingzhe
authors.initials: T
authors.lastname: Sun
authors.affiliation: State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing, Jiangsu 210093, P.R. China.
authors.forename: Pingping
authors.initials: P
authors.lastname: Shen
authors.affiliation: Institute and Hospital of Stomatology, Nanjing University Medical School, Nanjing, Jiangsu 210008, P.R. China.
authors.forename: Weibin
authors.initials: W
authors.lastname: Sun
mesh_terms: D000293:Adolescent; D000975:Antioxidants / Q000008:administration & dosage*; D017209:Apoptosis / Q000187:drug effects; D001205:Ascorbic Acid / Q000008:administration & dosage*; D002470:Cell Survival / Q000187:drug effects; D002648:Child; D006801:Humans; D006861:Hydrogen Peroxide / Q000633:toxicity*; D018384:Oxidative Stress / Q000187:drug effects; D010513:Periodontal Ligament / Q000166:cytology* / Q000187:drug effects*; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D000975:Antioxidants; D017382:Reactive Oxygen Species; D006861:Hydrogen Peroxide; D001205:Ascorbic Acid