title: Investigating conversion of endplate chondrocytes induced by intermittent cyclic mechanical unconfined compression in three-dimensional cultures.
abstract: Mechanical stimulation is known to regulate the calcification of endplate chondrocytes. The ANK protein has a strong influence on anti-calcification by transports intracellular inorganic pyrophosphate (PPi) to the extracellular. It is known that TGF-β1 is able to induced Ank gene expression and protect chondrocyte calcification. Intermittent cyclic mechanical tension (ICMT) could induce calcification of endplate chondrocytes by decrease the expression of Ank gene. In this study, we investigated the relation of intermittent cyclic mechanical unconfined compression (ICMC) and Ank gene expression. We found that ICMC decreased the Ank gene expression in the endplate chondrocytes, and there was an decreased in the  TGF-β1 expression after ICMC stimulation. The Ank gene expression significantly increased when treated by transforming growth factor alpha 1 (TGF-β1) in a dose-dependent manner and decreased when treated by SB431542 (ALK inhibitor) in a dose-dependent manner. Our results implicate that ICMC-induced downregulation of Ank gene expression may be regulated by TGF-β1 in endplate chondrocytes.
authors.affiliation: Wannan Medical College. xuhg@medmail.com.cn.
authors.forename: H G
authors.initials: HG
authors.lastname: Xu
authors.forename: W
authors.initials: W
authors.lastname: Zhang
authors.forename: Q
authors.initials: Q
authors.lastname: Zheng
authors.forename: Y F
authors.initials: YF
authors.lastname: Yu
authors.forename: L F
authors.initials: LF
authors.lastname: Deng
authors.forename: H
authors.initials: H
authors.lastname: Wang
authors.forename: P
authors.initials: P
authors.lastname: Liu
authors.forename: M
authors.initials: M
authors.lastname: Zhang
mesh_terms: D000818:Animals; D002113:Calcification, Physiologic; D018929:Cell Culture Techniques; D049109:Cell Proliferation; D002470:Cell Survival; D002478:Cells, Cultured; D019902:Chondrocytes / Q000166:cytology* / Q000378:metabolism*; D019245:Compressive Strength; D004797:Enzyme-Linked Immunosorbent Assay; D028061:Phosphate Transport Proteins / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D060888:Real-Time Polymerase Chain Reaction; D053773:Transforming Growth Factor beta1 / Q000378:metabolism
chemical_list: C498769:Ankh protein, mouse; D028061:Phosphate Transport Proteins; D053773:Transforming Growth Factor beta1