title: Continuous release of bFGF from multilayer nanofilm to maintain undifferentiated human iPS cell cultures.
abstract: In most cases, induced pluripotent stem (iPS) cells must be maintained by replacing basic fibroblast growth factor (bFGF)-containing medium daily to inhibit spontaneous differentiation. However, complete inhibition of differentiation is difficult to achieve even with frequent replacement of medium because of bFGF instability. Here, we established a novel system for the sustained release of bFGF from a modified surface by using a nanofilm fabrication method.
authors.affiliation: Department of Psychiatry and McLean Hospital, Harvard Medical School, Belmont, Massachusetts 02478, USA.
authors.forename: Ju Hyun
authors.initials: JH
authors.lastname: Park
authors.forename: Jinkee
authors.initials: J
authors.lastname: Hong
mesh_terms: D061249:Batch Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D003692:Delayed-Action Preparations / Q000008:administration & dosage / Q000138:chemical synthesis*; D016222:Fibroblast Growth Factor 2 / Q000008:administration & dosage* / Q000737:chemistry; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology / Q000187:drug effects* / Q000502:physiology*; D008422:Materials Testing; D008567:Membranes, Artificial; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure
chemical_list: D003692:Delayed-Action Preparations; D008567:Membranes, Artificial; D016222:Fibroblast Growth Factor 2