title: Mapping proteoglycan functions with glycosidases.
abstract: The intrinsic and extrinsic factors that contribute to stem and neuronal precursor cell maintenance and/or differentiation remain poorly understood. Proteoglycans, major residents of the stem cell microenvironment, modulate key signaling cues and are of particular importance. We have taken a loss-of-function approach, by developing a library of bacterial lyases and sulfatases to specifically remodel the ECM and test the functional role of glycosaminoglycans (GAGs) in cell self-renewal, maintenance, and differentiation.
authors.affiliation: Department of Pediatrics, University of Chicago, 5841S. Maryland Avenue, Chicago, IL, 60637, USA.
authors.forename: Mauricio
authors.initials: M
authors.lastname: Cortes
authors.forename: Leslie K
authors.initials: LK
authors.lastname: Cortes
authors.forename: Nancy B
authors.initials: NB
authors.lastname: Schwartz
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001671:Biochemistry / Q000379:methods*; D002454:Cell Differentiation; D049109:Cell Proliferation; D002540:Cerebral Cortex / Q000378:metabolism; D002810:Chondroitinases and Chondroitin Lyases / Q000235:genetics; D003001:Cloning, Molecular; D004586:Electrophoresis; D053595:Embryonic Stem Cells / Q000166:cytology / Q000378:metabolism; D000939:Epitopes / Q000378:metabolism; D005456:Fluorescent Dyes / Q000737:chemistry; D006025:Glycosaminoglycans / Q000378:metabolism; D006026:Glycoside Hydrolases / Q000378:metabolism*; D051379:Mice; D008969:Molecular Sequence Data; D015202:Protein Engineering; D021382:Protein Sorting Signals; D011509:Proteoglycans / Q000378:metabolism*
chemical_list: D000939:Epitopes; D005456:Fluorescent Dyes; D006025:Glycosaminoglycans; D021382:Protein Sorting Signals; D011509:Proteoglycans; D006026:Glycoside Hydrolases; D002810:Chondroitinases and Chondroitin Lyases