title: Animal model of chronic kidney disease using a unilateral technique of renal ischemia and reperfusion in White New Zealand rabbits.
abstract: OBJECTIVE
  To establish a model of chronic kidney disease in White New Zealand rabbits, using an exclusive unilateral technique of renal ischemia and reperfusion.
  
  
  METHODS
  Twenty males White New Zealand rabbits were used. All animals were subjected to the following: pre-surgical blood collection (1st collection) for creatinine and urea serum analysis, left renal ischemia and reperfusion surgery technique, another blood sample was collected after 6 weeks post surgery (2nd collection), the last blood sample (3rd collection) blood sample was taken 11 weeks post surgery (pre-euthanasia), euthanasia and withdrawal of right and left kidney for histopathological analysis.
  
  
  RESULTS
  The creatinine levels after surgery was statistically significant higher in the 3rd collection, regarding the 1st and 2nd collection (p<0.05). A significant statistic increase for urea was showed only in the 2nd collection (p<0.05) when compared to the 1st and 3rd collections. Histopathological analysis showed bilateral lesions in the renal tissue, consistent to the process of ischemia and reperfusion.
  
  
  CONCLUSIONS
  This exclusive unilateral technique of renal ischemia and reperfusion without nephrectomy in White New Zealand rabbits, showed effectiveness in getting an animal model of chronic kidney disease.
authors.affiliation: Federal University of Mato Grosso do Sul, Campo Grande, MS, Brazil.
authors.forename: Eliane Barbosa
authors.initials: EB
authors.lastname: Togoe
authors.affiliation: UFMS, Campo Grande, MS, Brazil.
authors.forename: Iandara Schettert
authors.initials: IS
authors.lastname: Silva
authors.affiliation: UFMS, Campo Grande, MS, Brazil.
authors.forename: Juliana Loprete
authors.initials: JL
authors.lastname: Cury
authors.affiliation: Department of Morphophysiology, UFMS, Campo Grande, MS, Brazil.
authors.forename: Albert Schiaveto
authors.initials: AS
authors.lastname: Souza
authors.affiliation: UNIGRAN, Dourados, MS, Brazil.
authors.forename: José Henrique Saraiva
authors.initials: JH
authors.lastname: Borges
authors.affiliation: Department of Veterinary, UNIGRAN, Dourados, MS, Brazil.
authors.forename: Klaus Casaro
authors.initials: KC
authors.lastname: Saturnino
mesh_terms: D000818:Animals; D003404:Creatinine / Q000097:blood; D004195:Disease Models, Animal; D007668:Kidney / Q000098:blood supply* / Q000473:pathology; D008297:Male; D011817:Rabbits; D051436:Renal Insufficiency, Chronic / Q000209:etiology* / Q000473:pathology*; D015427:Reperfusion Injury / Q000473:pathology*; D015203:Reproducibility of Results; D013997:Time Factors; D014508:Urea / Q000097:blood
chemical_list: D014508:Urea; D003404:Creatinine