title: A chromophore-containing agglutinin from Haliclona manglaris: purification and biochemical characterization.
abstract: A new chromophore-containing agglutinin (Haliclona manglaris agglutinin (HMA)) was isolated from the tropical sponge H. manglaris. HMA was purified by a combination of hydrophobic interaction chromatography and ion exchange chromatography. Native HMA is a heterotrimer formed by two β-chains (15 kDa) and one α-chain (22 kDa). HMA is a glycoprotein and possesses three intrachain disulfide bonds. Hemagglutinating activity of HMA was stable at neutral pH and temperatures up to 60 °C. HMA was only inhibited by thyroglobulin. Mass spectrometry sequencing and Edman degradation revealed a unique amino acid sequence of about 30%. Moreover, HMA has an organic chromophore of 581 Da, and this characteristic seems to be important to its antioxidant activity. Interestingly, while HMA showed no toxicity against Artemia nauplii and was unable to agglutinate bacterial cells, it did show a high capacity to protect β-carotene against oxidation. Thus, our findings suggest the putative involvement of HMA in the protection of the sponge against oxidation.
authors.forename: Rômulo Farias
authors.initials: RF
authors.lastname: Carneiro
authors.forename: Alexandra Sampaio
authors.initials: AS
authors.lastname: de Almeida
authors.forename: Arthur Alves
authors.initials: AA
authors.lastname: de Melo
authors.forename: Daniel Barroso
authors.initials: DB
authors.lastname: de Alencar
authors.forename: Oscarina Viana
authors.initials: OV
authors.lastname: de Sousa
authors.forename: Plínio
authors.initials: P
authors.lastname: Delatorre
authors.forename: Kyria Santiago
authors.initials: KS
authors.lastname: do Nascimento
authors.forename: Silvana
authors.initials: S
authors.lastname: Saker-Sampaio
authors.forename: Benildo Sousa
authors.initials: BS
authors.lastname: Cavada
authors.forename: Celso Shiniti
authors.initials: CS
authors.lastname: Nagano
authors.forename: Alexandre Holanda
authors.initials: AH
authors.lastname: Sampaio
mesh_terms: D000373:Agglutinins / Q000737:chemistry* / Q000302:isolation & purification*; D000595:Amino Acid Sequence; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D001156:Artemia / Q000187:drug effects; D002241:Carbohydrates / Q000032:analysis; D002413:Cations, Divalent / Q000494:pharmacology; D002850:Chromatography, Gel; D056148:Chromatography, Reverse-Phase; D004591:Electrophoresis, Polyacrylamide Gel; D005456:Fluorescent Dyes / Q000737:chemistry*; D047129:Haliclona / Q000737:chemistry*; D006384:Hemagglutination / Q000187:drug effects; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010455:Peptides / Q000737:chemistry; D011817:Rabbits; D020539:Sequence Analysis, Protein; D013438:Sulfhydryl Compounds / Q000737:chemistry; D053719:Tandem Mass Spectrometry; D013696:Temperature
chemical_list: D000373:Agglutinins; D000975:Antioxidants; D002241:Carbohydrates; D002413:Cations, Divalent; D005456:Fluorescent Dyes; D010455:Peptides; D013438:Sulfhydryl Compounds