title: Characterization of PHB1 and its role in mitochondrial maturation and yolk platelet degradation during development of Artemia embryos.
abstract: BACKGROUND
  To cope with harsh environments, crustaceans such as Artemia produce diapause gastrula embryos (cysts) with suppressed metabolism. Metabolism and development resume during post-diapause development, but the mechanism behind these cellular events remains largely unknown.
  
  
  RESULTS
  Our study investigated the role of prohibitin 1 (PHB1) in metabolic reinitiation during post-diapause development. We found that PHB1 was developmentally regulated via changes in phosphorylation status and localization. Results from RNA interference experiments demonstrated PHB1 to be critical for mitochondrial maturation and yolk degradation during development. In addition, PHB1 was present in yolk platelets, and it underwent ubiquitin-mediated degradation during the proteolysis of yolk protein.
  
  
  CONCLUSIONS
  PHB1 has an indispensable role in coordinating mitochondrial maturation and yolk platelet degradation during development in Artemia. This novel function of PHB1 provides new clues to comprehend the roles of PHB1 in metabolism and development.
authors.affiliation: Key Laboratory of Conservation Biology for Endangered Wildlife of the Ministry of Education and College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang, People's Republic of China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Ye
authors.affiliation: Department of Molecular and Cell Biology, University of California, Berkeley, California, United States of America.
authors.forename: Yang
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Key Laboratory of Conservation Biology for Endangered Wildlife of the Ministry of Education and College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang, People's Republic of China.
authors.forename: Ling-Ling
authors.initials: LL
authors.lastname: Zhao
authors.affiliation: Key Laboratory of Conservation Biology for Endangered Wildlife of the Ministry of Education and College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang, People's Republic of China.
authors.forename: Yu-Xia
authors.initials: YX
authors.lastname: Sun
authors.affiliation: Key Laboratory of Conservation Biology for Endangered Wildlife of the Ministry of Education and College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang, People's Republic of China.
authors.forename: Jin-Shu
authors.initials: JS
authors.lastname: Yang
authors.affiliation: Key Laboratory of Conservation Biology for Endangered Wildlife of the Ministry of Education and College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang, People's Republic of China.
authors.forename: Wei-Jun
authors.initials: WJ
authors.lastname: Yang
mesh_terms: D000818:Animals; D001156:Artemia; D001792:Blood Platelets / Q000378:metabolism*; D002467:Cell Nucleus / Q000235:genetics / Q000378:metabolism; D004625:Embryo, Nonmammalian / Q000378:metabolism*; D047108:Embryonic Development / Q000502:physiology*; D008928:Mitochondria / Q000235:genetics / Q000378:metabolism*; D000091402:Prohibitins; D034622:RNA Interference; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D000091402:Prohibitins; D012097:Repressor Proteins