title: Flexible gold electrode array for multiplexed immunoelectrochemical measurement of three protein biomarkers for prostate cancer.
abstract: In this work, we report a simple and novel electrochemical multiplexed immunosensor on a flexible polydimethylsiloxane (PDMS) slice deposited with 8 × 8 nano-Au film electrodes for simultaneous detection of prostate specific antigen (PSA), prostate specific membrane antigen (PSMA), and interleukin-6 (IL-6). Primary antibodies linked with magnetic beads (Ab1-MBs) were modified on the nano-Au film electrodes via magnetic force. In the presence of corresponding antigen, horse radish peroxidase-secondary antibody-conjugated gold nanorods (HRP-Ab2-gold NRs) were brought into the surface of electrodes, generating obvious electrochemical signals of H2O2 reduction reactions. Based on this, the designed immunosensor provide good performance in sensitivity and specificity during the detection of above three biomarkers for prostate cancer. The electrochemical multiplexed immunosensor was verified for selective and accurate detection of complex samples in human serum. Data suggested that the reported multiplexed immunosensing strategy holds great promise for applications in clinical assay and diseases diagnosis.
authors.affiliation: State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University , Nanjing 210093, P. R. China.
authors.forename: Jing
authors.initials: J
authors.lastname: Liu
authors.forename: Cai-Yu
authors.initials: CY
authors.lastname: Lu
authors.forename: Hong
authors.initials: H
authors.lastname: Zhou
authors.forename: Jing-Juan
authors.initials: JJ
authors.lastname: Xu
authors.forename: Hong-Yuan
authors.initials: HY
authors.lastname: Chen
mesh_terms: D055910:Antibodies, Immobilized / Q000276:immunology; D000954:Antigens, Surface / Q000032:analysis* / Q000276:immunology; D015415:Biomarkers / Q000032:analysis*; D004129:Dimethylpolysiloxanes / Q000737:chemistry; D055664:Electrochemical Techniques; D004566:Electrodes; D043425:Glutamate Carboxypeptidase II / Q000032:analysis* / Q000276:immunology; D006046:Gold; D006801:Humans; D006861:Hydrogen Peroxide / Q000737:chemistry / Q000378:metabolism; D007118:Immunoassay; D015850:Interleukin-6 / Q000032:analysis* / Q000276:immunology; D008280:Magnetics; D008297:Male; D046208:Microchip Analytical Procedures; D043942:Nanotubes / Q000737:chemistry; D010544:Peroxidases / Q000378:metabolism; D017430:Prostate-Specific Antigen / Q000032:analysis* / Q000276:immunology; D011471:Prostatic Neoplasms / Q000175:diagnosis* / Q000378:metabolism
chemical_list: D055910:Antibodies, Immobilized; D000954:Antigens, Surface; D015415:Biomarkers; D004129:Dimethylpolysiloxanes; D015850:Interleukin-6; C013830:baysilon; D006046:Gold; D006861:Hydrogen Peroxide; D010544:Peroxidases; C473848:FOLH1 protein, human; D043425:Glutamate Carboxypeptidase II; D017430:Prostate-Specific Antigen
keywords: electrochemical immunosensor; flexible electrode; microchip array; multiplexed measurement; prostate cancer