title: Pharmacokinetics of two alkaloids after oral administration of rhizoma coptidis extract in normal rats and irritable bowel syndrome rats.
abstract: A comparative pharmacokinetic study of berberine and palmatine after oral administration of Rhizoma Coptidis extract (96 mg/kg, containing berberine 22 mg/kg and palmatine 5 mg/kg based on body weight) was performed in normal and postinflammation irritable bowel syndrome (PI-IBS) rats, induced by intracolonic instillation of acetic acid and restraint stress. Quantification of berberine and palmatine in rat plasma was achieved by using a sensitive and rapid UPLC-MS/MS method. Plasma samples were collected at 13 different time points and the pharmacokinetic parameters were analyzed by WinNonlin software. The significant differences in the pharmacokinetic behaviors, such as C max⁡, AUC(0-t), V d /F, and CL/F, of berberine and palmatine were found between normal and PI-IBS model rats. The results indicated that PI-IBS pathological conditions in rats could alter the pharmacokinetic behavior of drug. Preclinical pharmacokinetic studies are usually carried out on healthy animals. However, we should pay more attention to the fact that the change of pharmacokinetic behavior plays an important role on efficacy. It is essential to investigate the pharmacokinetics of the drug in disease status.
authors.affiliation: Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, No. 16, Dongzhimen Nei Nanxiao Road, Dongcheng District, Beijing 100700, China.
authors.forename: Zipeng
authors.initials: Z
authors.lastname: Gong
authors.affiliation: Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, No. 16, Dongzhimen Nei Nanxiao Road, Dongcheng District, Beijing 100700, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, No. 16, Dongzhimen Nei Nanxiao Road, Dongcheng District, Beijing 100700, China.
authors.forename: Ruijie
authors.initials: R
authors.lastname: Zhang
authors.affiliation: Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, No. 16, Dongzhimen Nei Nanxiao Road, Dongcheng District, Beijing 100700, China ; Institute of Chinese Materia Medica, Chengde Medical University, Chengde 067000, China.
authors.forename: Yinghan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, No. 16, Dongzhimen Nei Nanxiao Road, Dongcheng District, Beijing 100700, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Yang
authors.affiliation: Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, No. 16, Dongzhimen Nei Nanxiao Road, Dongcheng District, Beijing 100700, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Guo
authors.affiliation: Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, No. 16, Dongzhimen Nei Nanxiao Road, Dongcheng District, Beijing 100700, China.
authors.forename: Xiaogang
authors.initials: X
authors.lastname: Weng
authors.affiliation: Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, No. 16, Dongzhimen Nei Nanxiao Road, Dongcheng District, Beijing 100700, China.
authors.forename: Shuangrong
authors.initials: S
authors.lastname: Gao
authors.affiliation: Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, No. 16, Dongzhimen Nei Nanxiao Road, Dongcheng District, Beijing 100700, China.
authors.forename: Hailin
authors.initials: H
authors.lastname: Wang
authors.affiliation: Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, No. 16, Dongzhimen Nei Nanxiao Road, Dongcheng District, Beijing 100700, China.
authors.forename: Xiaoxin
authors.initials: X
authors.lastname: Zhu
authors.affiliation: Guang'an Men Hospital, China Academy of Chinese Medical Sciences, No. 5, Beixiange Road, Xicheng District, Beijing 100053, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Dong
authors.affiliation: Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, No. 16, Dongzhimen Nei Nanxiao Road, Dongcheng District, Beijing 100700, China.
authors.forename: Yujie
authors.initials: Y
authors.lastname: Li
authors.affiliation: Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, No. 16, Dongzhimen Nei Nanxiao Road, Dongcheng District, Beijing 100700, China.
authors.forename: Yajie
authors.initials: Y
authors.lastname: Wang