title: Atomic force microscopy measurements reveal multiple bonds between Helicobacter pylori blood group antigen binding adhesin and Lewis b ligand.
abstract: The strength of binding between the Helicobacter pylori blood group antigen-binding adhesin (BabA) and its cognate glycan receptor, the Lewis b blood group antigen (Le(b)), was measured by means of atomic force microscopy. High-resolution measurements of rupture forces between single receptor-ligand pairs were performed between the purified BabA and immobilized Le(b) structures on self-assembled monolayers. Dynamic force spectroscopy revealed two similar but statistically different bond populations. These findings suggest that the BabA may form different adhesive attachments to the gastric mucosa in ways that enhance the efficiency and stability of bacterial adhesion.
authors.affiliation: INEB-Instituto de Engenharia Biomédica, Universidade do Porto, Porto, Portugal.
authors.forename: P
authors.initials: P
authors.lastname: Parreira
authors.affiliation: Department of Chemical and Biomolecular Engineering, University of Illinois at Urbana-Champaign, Urbana, IL, USA.
authors.forename: Q
authors.initials: Q
authors.lastname: Shi
authors.affiliation: IPATIMUP-Instituto de Patologia e Imunologia Molecular da Universidade do Porto, Porto, Portugal.
authors.forename: A
authors.initials: A
authors.lastname: Magalhaes
authors.affiliation: IPATIMUP-Instituto de Patologia e Imunologia Molecular da Universidade do Porto, Porto, Portugal Faculdade de Medicina, Universidade do Porto, Porto, Portugal Instituto de Ciências Biomédicas Abel Salazar, Universidade do Porto, Porto, Portugal.
authors.forename: C A
authors.initials: CA
authors.lastname: Reis
authors.affiliation: Department of Medical Biochemistry and Biophysics, Umeå University, Umeå, Sweden.
authors.forename: J
authors.initials: J
authors.lastname: Bugaytsova
authors.affiliation: Department of Medical Biochemistry and Biophysics, Umeå University, Umeå, Sweden.
authors.forename: T
authors.initials: T
authors.lastname: Borén
authors.affiliation: Department of Chemical and Biomolecular Engineering, University of Illinois at Urbana-Champaign, Urbana, IL, USA.
authors.forename: D
authors.initials: D
authors.lastname: Leckband
authors.affiliation: INEB-Instituto de Engenharia Biomédica, Universidade do Porto, Porto, Portugal Instituto de Ciências Biomédicas Abel Salazar, Universidade do Porto, Porto, Portugal cmartins@ineb.up.pt.
authors.forename: M C L
authors.initials: MC
authors.lastname: Martins
mesh_terms: D018829:Adhesins, Bacterial / Q000737:chemistry / Q000378:metabolism*; D001422:Bacterial Adhesion / Q000502:physiology*; D005753:Gastric Mucosa / Q000737:chemistry / Q000378:metabolism / Q000382:microbiology; D016480:Helicobacter pylori / Q000378:metabolism* / Q000648:ultrastructure; D006801:Humans; D007983:Lewis Blood Group Antigens; D018625:Microscopy, Atomic Force; D009844:Oligosaccharides / Q000737:chemistry / Q000378:metabolism*
chemical_list: C500822:3-aminopropyl fucopyranosyl-1-2-galactopyranosyl-1-3-(fucopyranosyl-1-4)-2-acetamido-2-deoxyglucopyranosyl-1-3-galactopyranosyl-1-4-glucopyranoside; D018829:Adhesins, Bacterial; C400429:BabA protein, Helicobacter pylori; D007983:Lewis Blood Group Antigens; D009844:Oligosaccharides
keywords: Helicobacter pylori; Lewis b; adhesion force; atomic force microscopy; blood group antigen-binding adhesin; self-assembled monolayers