title: Mucosal vaccination against tuberculosis using Ag85A-loaded immunostimulating complexes.
abstract: Tuberculosis (TB) is one of the major devastating diseases in the world, mainly caused by Mycobacterium tuberculosis. Furthermore, multi-drug resistant TB and extremely drug resistant TB are becoming big problems globally. Bacillus Calmette-Guerin (BCG) is the only available vaccine which provides protection against TB. The BCG vaccine is effective in children but not recommended in adults and elderly patients due to an associated low risk of infection with Mycobacterium tuberculosis and variable effectiveness of the vaccine. The main aim of this research study is to develop such a vaccine which will provide a better and safer profile in children and adults, as well as in elderly patients. In this present study, we prepared pulmonary tubercular vaccine by using an Antigen 85 complex (Ag85)-loaded nanocarrier such as the immunostimulating complex (ISCOM). Immunological outcomes clearly indicated significant improvement in humoral as well as cellular immune responses after pulmonary immunization with ISCOMs containing Quil A in mice.
authors.affiliation: a Department of Pharmaceutics , ISF College of Pharmacy , Moga , Punjab , India.
authors.forename: Swati
authors.initials: S
authors.lastname: Pabreja
authors.affiliation: a Department of Pharmaceutics , ISF College of Pharmacy , Moga , Punjab , India.
authors.forename: Tarun
authors.initials: T
authors.lastname: Garg
authors.affiliation: a Department of Pharmaceutics , ISF College of Pharmacy , Moga , Punjab , India.
authors.forename: Goutam
authors.initials: G
authors.lastname: Rath
authors.affiliation: a Department of Pharmaceutics , ISF College of Pharmacy , Moga , Punjab , India.
authors.forename: Amit K
authors.initials: AK
authors.lastname: Goyal
mesh_terms: D000217:Acyltransferases / Q000737:chemistry* / Q000276:immunology*; D000818:Animals; D000942:Antigens, Bacterial / Q000737:chemistry* / Q000276:immunology*; D018938:Caco-2 Cells; D002470:Cell Survival / Q000187:drug effects; D004337:Drug Carriers / Q000737:chemistry* / Q000633:toxicity; D065546:Drug Liberation; D005260:Female; D006461:Hemolysis / Q000187:drug effects; D006801:Humans; D016882:ISCOMs / Q000737:chemistry* / Q000276:immunology / Q000633:toxicity; D007132:Immunoglobulin Isotypes / Q000097:blood / Q000737:chemistry; D051379:Mice; D009092:Mucous Membrane / Q000276:immunology; D009169:Mycobacterium tuberculosis / Q000276:immunology / Q000502:physiology; D049329:Nanostructures / Q000737:chemistry; D014397:Tuberculosis, Pulmonary / Q000517:prevention & control*; D014611:Vaccination
chemical_list: D000942:Antigens, Bacterial; D004337:Drug Carriers; D016882:ISCOMs; D007132:Immunoglobulin Isotypes; D000217:Acyltransferases; C112580:antigen 85A, Mycobacterium tuberculosis
keywords: Quil A; immune response; immunostimulating complex; mucosal vaccine; reverse phase evaporation