title: [Immunosuppressive effects of fetal bone marrow derived mesenchymal stem cells on in vitro proliferation of adult peripheral lymphocyte and expression of immune-related factors].
abstract: OBJECTIVE
  To investigate the potential immunomodulatory properties of fetal bone marrow derived mesenchymal stem cells (FBM- MSCs).
  
  
  METHODS
  Mononuclear cells from the bone marrow of second trimester (14-22 wks) fetus were isolated and cultured for the derivation of MSCs. The derived FBM-MSC cells were characterized via morphology, immunophenotyping and the adipogenic and osteogenic differentiation assays. The immunomodulatory properties of FBM-MSC on lymphocytes were evaluated through the co- culture assay with PHA activated adult peripheral blood mononuclear cells (PBMCs).
  
  
  RESULTS
  Derived FBM-MSCs were CD29⁺, CD44⁺, CD49e⁺, CD73⁺, CD90⁺, CD105⁺ and CD31⁻ , CD34⁻ , CD45⁻ , HLA-DR⁻ and can be differentiated into adipocytes and osteocytes. When co-cultured with PHA-activated PBMCs, FBM-MSCs inhibited the proliferation of lymphocytes up to 96% and down-regulated the secretion of inflammatory cytokines such as IFN-γ and TNF-α up to 90.9% and 58.4% respectively. When compared with FBM-MSCs cultured alone, the expression of MSCs derived immunomodulatory cytokines, such as IDO, TSG-6 and TGF-β, was up-regulated significantly in the co-culture system.
  
  
  CONCLUSIONS
  MSC derived from fetal bone marrow demonstrated immunosuppressive effects on adult PBMCs in vitro. MSC-derived cytokines like IDO, TSG-6 and TGF-β may be critical for FBM-MSCs mediated immunosuppressive function.
authors.affiliation: State Key Laboratory of Experimental Hematology, Institute of Hematology and Blood Diseases Hospital, PUMC and CAMS, Tianjin 300020, China; Central Laboratory, Jinan Central Hospital Affiliated to Shandong University, Jinan 250013, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Li
authors.affiliation: State Key Laboratory of Experimental Hematology, Institute of Hematology and Blood Diseases Hospital, PUMC and CAMS, Tianjin 300020, China; Central Laboratory, Jinan Central Hospital Affiliated to Shandong University, Jinan 250013, China.
authors.forename: Junqiang
authors.initials: J
authors.lastname: Lyu
authors.affiliation: State Key Laboratory of Experimental Hematology, Institute of Hematology and Blood Diseases Hospital, PUMC and CAMS, Tianjin 300020, China; Central Laboratory, Jinan Central Hospital Affiliated to Shandong University, Jinan 250013, China.
authors.forename: Yongjuan
authors.initials: Y
authors.lastname: Duan
authors.affiliation: State Key Laboratory of Experimental Hematology, Institute of Hematology and Blood Diseases Hospital, PUMC and CAMS, Tianjin 300020, China; Central Laboratory, Jinan Central Hospital Affiliated to Shandong University, Jinan 250013, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Sun
authors.affiliation: State Key Laboratory of Experimental Hematology, Institute of Hematology and Blood Diseases Hospital, PUMC and CAMS, Tianjin 300020, China; Central Laboratory, Jinan Central Hospital Affiliated to Shandong University, Jinan 250013, China.
authors.forename: Dong
authors.initials: D
authors.lastname: Li
authors.affiliation: State Key Laboratory of Experimental Hematology, Institute of Hematology and Blood Diseases Hospital, PUMC and CAMS, Tianjin 300020, China; Central Laboratory, Jinan Central Hospital Affiliated to Shandong University, Jinan 250013, China.
authors.forename: Yunshan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Experimental Hematology, Institute of Hematology and Blood Diseases Hospital, PUMC and CAMS, Tianjin 300020, China; Central Laboratory, Jinan Central Hospital Affiliated to Shandong University, Jinan 250013, China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Hu
authors.affiliation: State Key Laboratory of Experimental Hematology, Institute of Hematology and Blood Diseases Hospital, PUMC and CAMS, Tianjin 300020, China; Central Laboratory, Jinan Central Hospital Affiliated to Shandong University, Jinan 250013, China.
authors.forename: Dongjie
authors.initials: D
authors.lastname: Xiao
mesh_terms: D000328:Adult; D001853:Bone Marrow; D001854:Bone Marrow Cells / Q000166:cytology / Q000276:immunology*; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D018920:Coculture Techniques; D016207:Cytokines; D006412:Hematopoietic Stem Cells; D006801:Humans; D007108:Immune Tolerance; D016130:Immunophenotyping; D066298:In Vitro Techniques; D007963:Leukocytes, Mononuclear; D008214:Lymphocytes; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000276:immunology*; D010012:Osteogenesis
chemical_list: D016207:Cytokines