title: Development of surface plasmon resonance imaging biosensors for detection of ubiquitin carboxyl-terminal hydrolase L1.
abstract: We have developed a new method for highly selective determination of the ubiquitin carboxyl-terminal hydrolase L1 (UCH-L1) concentration using a surface plasmon resonance imaging (SPRI) technique and two different biosensors. UCH-L1 was captured from a solution by immobilized specific rabbit monoclonal antibody or specific LDN-57444 inhibitor due to formation of receptor-UCH-L1 complex on the biosensor surface. The analytically useful dynamic response range of both biosensors is between 0.1 and 2.5ng/ml. The detection limit is 0.06ng/ml for the biosensor with antibody and 0.08ng/ml for the biosensor with inhibitor. Biosensors based on both antibody and inhibitor were found to be suitable for quantitative determination of the UCH-L1 and exhibit good tolerance to the potential interferents. Both biosensors gave comparable results in the range of 0 to 0.20ng/ml for plasma samples and 0.30 to 0.49ng/ml for cerebrospinal fluid samples. To validate the new methods, comparative determination of UCH-L1 by the commercial enzyme-linked immunosorbent assay (ELISA) kit was performed. In general, in terms of UCH-L1 concentration, a good correlation between SPRI and ELISA was found. The developed biosensors can be used successfully for the determination of UCH-L1 in body fluids.
authors.affiliation: Department of Electrochemistry, Institute of Chemistry, University of Bialystok, 15-443 Bialystok, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Sankiewicz
authors.affiliation: Department of Perinatology, Medical University of Bialystok, 15-276 Bialystok, Poland.
authors.forename: Piotr
authors.initials: P
authors.lastname: Laudanski
authors.affiliation: Department of Medical Biochemistry, Medical University of Bialystok, 15-089 Bialystok, Poland.
authors.forename: Lech
authors.initials: L
authors.lastname: Romanowicz
authors.affiliation: Department of Pediatric Surgery, Medical University of Bialystok, 15-274 Bialystok, Poland.
authors.forename: Adam
authors.initials: A
authors.lastname: Hermanowicz
authors.affiliation: Department of Analytical Chemistry, Medical University of Bialystok, 15-230 Bialystok, Poland.
authors.forename: Wiesława
authors.initials: W
authors.lastname: Roszkowska-Jakimiec
authors.affiliation: Department of Pediatric Surgery, Medical University of Bialystok, 15-274 Bialystok, Poland.
authors.forename: Wojciech
authors.initials: W
authors.lastname: Debek
authors.affiliation: Department of Electrochemistry, Institute of Chemistry, University of Bialystok, 15-443 Bialystok, Poland. Electronic address: ewka@uwb.edu.pl.
authors.forename: Ewa
authors.initials: E
authors.lastname: Gorodkiewicz
mesh_terms: D000818:Animals; D055910:Antibodies, Immobilized / Q000737:chemistry / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000737:chemistry / Q000276:immunology; D015374:Biosensing Techniques; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007211:Indoles / Q000737:chemistry / Q000378:metabolism; D010091:Oximes / Q000737:chemistry / Q000378:metabolism; D011817:Rabbits; D011994:Recombinant Proteins / Q000032:analysis / Q000096:biosynthesis / Q000378:metabolism; D020349:Surface Plasmon Resonance; D043222:Ubiquitin Thiolesterase / Q000032:analysis* / Q000097:blood / Q000276:immunology
chemical_list: D055910:Antibodies, Immobilized; D000911:Antibodies, Monoclonal; D007211:Indoles; C525086:LDN 57444; D010091:Oximes; D011994:Recombinant Proteins; C507533:Ubiquitin carboxyl-Terminal Hydrolase L-1, human; D043222:Ubiquitin Thiolesterase
keywords: Biosensor; Surface plasmon resonance imaging; Ubiquitin carboxyl-terminal hydrolase L1 (UCH-L1)