title: Novel genipin-collagen immobilization of polylactic acid (PLA) fibers for use as tissue engineering scaffolds.
abstract: The material surface plays an important role in the case of biomaterials used as tissue engineering scaffolds. On exposure to a biological environment, extra cellular matrix (ECM) proteins are adsorbed non-specifically onto the surface and cells interact indirectly with the surface through the adsorbed proteins. Most synthetic polymeric biomaterials lack the desirable surface properties for cells as well as have poor cellular adhesion due to their hydrophobic nature. The main objective of this study was to harness surface functionalization technologies to fabricate scaffolds that would be biocompatible and support the adhesion and proliferation of fibroblast cells. The collagen was immobilized on the surface of functionalized PLA via a novel natural cross-linking molecule genipin which resulted in improved cell proliferation of human dermal fibroblasts as compared to the PLA surface coated with collagen without genipin. It is believed that genipin helps reduce steric problems between the functional groups and large protein molecules, and enables immobilized peptide to move more freely in a biological environment.
authors.affiliation: College of Textiles, North Carolina State University, Raleigh, North Carolina.
authors.forename: Nisarg
authors.initials: N
authors.lastname: Tambe
authors.affiliation: College of Textiles, North Carolina State University, Raleigh, North Carolina.|Department of Biomedical Engineering, North Carolina State University, Raleigh, North Carolina.
authors.forename: Jin
authors.initials: J
authors.lastname: Di
authors.affiliation: Department of Surgery, Faculty of Medicine, Laval University, Quebec, Quebec, Canada.
authors.forename: Ze
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Department of Biomedical Engineering, North Carolina State University, Raleigh, North Carolina.
authors.forename: Susan
authors.initials: S
authors.lastname: Bernacki
authors.affiliation: College of Textiles, North Carolina State University, Raleigh, North Carolina.
authors.forename: Ahmed
authors.initials: A
authors.lastname: El-Shafei
authors.affiliation: College of Textiles, North Carolina State University, Raleigh, North Carolina.
authors.forename: Martin W
authors.initials: MW
authors.lastname: King
mesh_terms: D002478:Cells, Cultured; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D039823:Iridoids / Q000737:chemistry*; D019344:Lactic Acid / Q000737:chemistry*; D008422:Materials Testing; D011091:Polyesters; D011108:Polymers / Q000737:chemistry*; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D039823:Iridoids; D011091:Polyesters; D011108:Polymers; D019344:Lactic Acid; C033616:poly(lactide); C007834:genipin
keywords: collagen immobilization; functionalization; genipin; polylactic acid (PLA) fibers; scaffold; surface modification