title: Sinusoidal electromagnetic fields promote bone formation and inhibit bone resorption in rat femoral tissues in vitro.
abstract: Effects of sinusoidal electromagnetic fields (SEMFs) on bone metabolism have not yet been well defined. The present study investigated SEMF effects on bone formation and resorption in rat femur bone tissues in vitro. Cultured femur diaphyseal (cortical bone) and metaphyseal (trabecular bone) tissues were treated with 50 Hz 1.8 mT SEMFs 1.5 h per day for up to 12 days and treatment effects on bone formation and resorption markers and associated gene expression were examined. Treatment with SEMFs caused a significant increase in alkaline phosphatase (ALP) activity and inhibited the tartrate-resistant acid phosphatase (TRACP) activity in the femoral diaphyseal or metaphyseal tissues. SEMFs also significantly increased levels of mRNA expression of osterix (OSX), insulin-like growth factor (IGF-1) and ALP in the bone tissues. SEMF treatment decreased glucose content and increased lactic acid contents in the culture conditioned medium. In addition, treatment with SEMFs decreased mRNA expression levels of bone resorption-related genes TRACP, macrophage colony stimulating factor (M-CSF) and cathepsin K (CTSK) in the cultured bone tissues. In conclusion, the current study demonstrated that treatment with 1.8 mT SEMFs at 1.5 h per day promoted bone formation, increased metabolism and inhibited resorption in both metaphyseal and diaphyseal bone tissues in vitro.
authors.affiliation: a Institute of Orthopaedics, Lanzhou General Hospital, Lanzhou Command of CPLA , Lanzhou , People's Republic of China and.
authors.forename: Jian
authors.initials: J
authors.lastname: Zhou
authors.affiliation: a Institute of Orthopaedics, Lanzhou General Hospital, Lanzhou Command of CPLA , Lanzhou , People's Republic of China and.
authors.forename: Xiao-Ni
authors.initials: XN
authors.lastname: Ma
authors.forename: Yu-Hai
authors.initials: YH
authors.lastname: Gao
authors.affiliation: a Institute of Orthopaedics, Lanzhou General Hospital, Lanzhou Command of CPLA , Lanzhou , People's Republic of China and.
authors.forename: Juan-Li
authors.initials: JL
authors.lastname: Yan
authors.affiliation: a Institute of Orthopaedics, Lanzhou General Hospital, Lanzhou Command of CPLA , Lanzhou , People's Republic of China and.
authors.forename: Wen-Gui
authors.initials: WG
authors.lastname: Shi
authors.affiliation: b Sansom Institute for Health Research, School of Pharmacy and Medical Sciences, University of South Australia , Adelaide , SA , Australia.
authors.forename: Cory J
authors.initials: CJ
authors.lastname: Xian
authors.affiliation: a Institute of Orthopaedics, Lanzhou General Hospital, Lanzhou Command of CPLA , Lanzhou , People's Republic of China and.
authors.forename: Ke-Ming
authors.initials: KM
authors.lastname: Chen
mesh_terms: D000135:Acid Phosphatase / Q000378:metabolism; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001862:Bone Resorption / Q000235:genetics / Q000378:metabolism / Q000503:physiopathology / Q000628:therapy*; D004574:Electromagnetic Fields; D005269:Femur / Q000378:metabolism / Q000502:physiology / Q000503:physiopathology / Q000528:radiation effects*; D005786:Gene Expression Regulation / Q000528:radiation effects; D005947:Glucose / Q000378:metabolism; D007527:Isoenzymes / Q000378:metabolism; D019344:Lactic Acid / Q000378:metabolism; D055909:Magnetic Field Therapy; D008297:Male; D010012:Osteogenesis / Q000235:genetics / Q000528:radiation effects*; D051381:Rats; D017208:Rats, Wistar; D000071681:Tartrate-Resistant Acid Phosphatase
chemical_list: D007527:Isoenzymes; D019344:Lactic Acid; D000469:Alkaline Phosphatase; D000135:Acid Phosphatase; D000071681:Tartrate-Resistant Acid Phosphatase; D005947:Glucose
keywords: Bone formation; bone organ culture; bone resorption; diaphysis; metaphysic; sinusoidal electromagnetic fields