title: Pertactin deficient Bordetella pertussis present a better fitness in mice immunized with an acellular pertussis vaccine.
abstract: Bordetella pertussis is the etiologic agent of whooping cough and has been the target of vaccination for over fifty years. The latest strategies include the use of acellular pertussis vaccines that induce specific immunity against few virulence factors amongst which pertactin is included in three and five component acellular pertussis vaccines. Recently, it has been reported that B. pertussis clinical isolates loose the production of this adhesin in regions reaching high vaccine coverage with vaccines targeting this virulence factor. We here demonstrate that isolates not producing pertactin are capable of sustaining longer infection as compared to pertactin producing isolates in an in vivo model of acellular pertussis immunization. Loosing pertactin production might thus provide a selective advantage to these isolates in this background, which could account for the upraise in prevalence of these pertactin deficient isolates in the population.
authors.affiliation: Institut Pasteur de Paris, Unité PTMMH, 25-28 rue du Dr Roux, F-75015 Paris, France; Centre National de la Recherche Scientifique, CNRS, URA3012 Paris, France.
authors.forename: N
authors.initials: N
authors.lastname: Hegerle
authors.affiliation: Institut Pasteur de Paris, Unité PTMMH, 25-28 rue du Dr Roux, F-75015 Paris, France; Centre National de la Recherche Scientifique, CNRS, URA3012 Paris, France.
authors.forename: G
authors.initials: G
authors.lastname: Dore
authors.affiliation: Institut Pasteur de Paris, Unité PTMMH, 25-28 rue du Dr Roux, F-75015 Paris, France; Centre National de la Recherche Scientifique, CNRS, URA3012 Paris, France. Electronic address: nicole.guiso@pasteur.fr.
authors.forename: N
authors.initials: N
authors.lastname: Guiso
mesh_terms: D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics / Q000276:immunology*; D001885:Bordetella Infections / Q000276:immunology* / Q000382:microbiology*; D001886:Bordetella pertussis / Q000235:genetics / Q000276:immunology*; D002353:Carrier State / Q000276:immunology* / Q000382:microbiology*; D005260:Female; D008807:Mice, Inbred BALB C; D023421:Models, Animal; D010567:Pertussis Vaccine / Q000008:administration & dosage / Q000276:immunology*; D022282:Vaccines, Acellular / Q000008:administration & dosage / Q000276:immunology; D010566:Virulence Factors, Bordetella / Q000235:genetics / Q000276:immunology*
chemical_list: D001425:Bacterial Outer Membrane Proteins; D010567:Pertussis Vaccine; D022282:Vaccines, Acellular; D010566:Virulence Factors, Bordetella; C068929:pertactin
keywords: Bordetella pertussis; Mice colonization; Pertactin deficiency; Whooping cough