title: Vulcanisaeta thermophila sp. nov., a hyperthermophilic and acidophilic crenarchaeon isolated from solfataric soil.
abstract: An anaerobic, rod-shaped, hyperthermophilic and acidophilic crenarchaeon, designated strain CBA1501(T), was isolated from solfataric soil of the Mayon volcano in the Republic of the Philippines. Phylogenetic analysis showed that strain CBA1501(T) is affiliated with the genus Vulcanisaeta in the phylum Crenarchaeota. DNA sequence similarities between the 16S rRNA gene of strain CBA1501(T) and those of Vulcanisaeta distributa IC-017(T) and Vulcanisaeta souniana IC-059(T) were 98.5 and 97.4 %, respectively. Strain CBA1501(T) grew between 75-90 °C, over a pH range of 4.0-6.0 and in the presence of 0-1.0 % (w/v) NaCl, with optimal growth occurring at 85 °C, pH 5.0, and with 0 % (w/v) NaCl. Fumarate, malate, oxidized glutathione, sulfur and thiosulfate were used as final electron acceptors, but FeCl3, nitrate and sulfate were not. The DNA G+C content of strain CBA1501(T) was 43.1 mol%. On the basis of polyphasic taxonomic analysis, strain CBA1501(T) represents a novel species of the genus Vulcanisaeta in the phylum Crenarchaeota, for which we propose the name Vulcanisaeta thermophila sp. nov. The type strain is CBA1501(T) ( = ATCC BAA-2415(T) = JCM 17228(T)).
authors.affiliation: Korea Basic Science Institute, Daejeon 305-806, Korea.
authors.forename: Kyung June
authors.initials: KJ
authors.lastname: Yim
authors.affiliation: Division of Bioengineering, Incheon National University, Incheon 406-772, Korea.|Korea Basic Science Institute, Daejeon 305-806, Korea.
authors.forename: In-Tae
authors.initials: IT
authors.lastname: Cha
authors.affiliation: Korea Basic Science Institute, Daejeon 305-806, Korea.
authors.forename: Jin-Kyu
authors.initials: JK
authors.lastname: Rhee
authors.affiliation: Korea Basic Science Institute, Daejeon 305-806, Korea.
authors.forename: Hye Seon
authors.initials: HS
authors.lastname: Song
authors.affiliation: Department of Life and Nanopharmaceutical Sciences and Department of Biology, Kyung Hee University, Seoul 130-701, Korea.
authors.forename: Dong-Wook
authors.initials: DW
authors.lastname: Hyun
authors.affiliation: Korea Basic Science Institute, Daejeon 305-806, Korea.
authors.forename: Hae-Won
authors.initials: HW
authors.lastname: Lee
authors.affiliation: Korea Basic Science Institute, Daejeon 305-806, Korea.
authors.forename: Daekyung
authors.initials: D
authors.lastname: Kim
authors.affiliation: Korea Basic Science Institute, Daejeon 305-806, Korea.
authors.forename: Kil-Nam
authors.initials: KN
authors.lastname: Kim
authors.affiliation: Fermentation and Functionality Research Group, Korea Food Research Institute, Sungnam 463-746, Korea.
authors.forename: Young-Do
authors.initials: YD
authors.lastname: Nam
authors.affiliation: Division of Bioengineering, Incheon National University, Incheon 406-772, Korea.
authors.forename: Myung-Ji
authors.initials: MJ
authors.lastname: Seo
authors.affiliation: Department of Life and Nanopharmaceutical Sciences and Department of Biology, Kyung Hee University, Seoul 130-701, Korea.
authors.forename: Jin-Woo
authors.initials: JW
authors.lastname: Bae
authors.affiliation: Korea Basic Science Institute, Daejeon 305-806, Korea.
authors.forename: Seong Woon
authors.initials: SW
authors.lastname: Roh
mesh_terms: D001482:Base Composition; D019615:Crenarchaeota / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D019641:DNA, Archaeal / Q000235:genetics; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010679:Philippines; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology
chemical_list: D019641:DNA, Archaeal; D012336:RNA, Ribosomal, 16S