title: Informatic system for a global tissue-fluid biorepository with a graph theory-oriented graphical user interface.
abstract: The Richard Floor Biorepository supports collaborative studies of extracellular vesicles (EVs) found in human fluids and tissue specimens. The current emphasis is on biomarkers for central nervous system neoplasms but its structure may serve as a template for collaborative EV translational studies in other fields. The informatic system provides specimen inventory tracking with bar codes assigned to specimens and containers and projects, is hosted on globalized cloud computing resources, and embeds a suite of shared documents, calendars, and video-conferencing features. Clinical data are recorded in relation to molecular EV attributes and may be tagged with terms drawn from a network of externally maintained ontologies thus offering expansion of the system as the field matures. We fashioned the graphical user interface (GUI) around a web-based data visualization package. This system is now in an early stage of deployment, mainly focused on specimen tracking and clinical, laboratory, and imaging data capture in support of studies to optimize detection and analysis of brain tumour-specific mutations. It currently includes 4,392 specimens drawn from 611 subjects, the majority with brain tumours. As EV science evolves, we plan biorepository changes which may reflect multi-institutional collaborations, proteomic interfaces, additional biofluids, changes in operating procedures and kits for specimen handling, novel procedures for detection of tumour-specific EVs, and for RNA extraction and changes in the taxonomy of EVs. We have used an ontology-driven data model and web-based architecture with a graph theory-driven GUI to accommodate and stimulate the semantic web of EV science.
authors.affiliation: Neurosurgical Service, Massachusetts General Hospital, Boston, MA, USA ; Massachusetts General Hospital, Boston, MA, USA.
authors.forename: William E
authors.initials: WE
authors.lastname: Butler
authors.affiliation: Neurosurgical Service, Massachusetts General Hospital, Boston, MA, USA ; Massachusetts General Hospital, Boston, MA, USA ; Department of Cell Biology and Histology, University of Amsterdam, Amsterdam, The Netherlands.
authors.forename: Nadia
authors.initials: N
authors.lastname: Atai
authors.affiliation: Department of Neurosurgery, University of San Diego Medical School, San Diego, CA, USA.
authors.forename: Bob
authors.initials: B
authors.lastname: Carter
authors.affiliation: Massachusetts General Hospital, Boston, MA, USA.
authors.forename: Fred
authors.initials: F
authors.lastname: Hochberg
keywords: biorepository; exosome; extracellular vesicle; graph theory; medical research database; ontology