title: Structure of the lysine specific protease Kgp from Porphyromonas gingivalis, a target for improved oral health.
abstract: The oral pathogen Porphyromonas gingivalis is a keystone pathogen in the development of chronic periodontitis. Gingipains, the principle virulence factors of P. gingivalis are multidomain, cell-surface proteins containing a cysteine protease domain. The lysine specific gingipain, Kgp, is a critical virulence factor of P. gingivalis. We have determined the X-ray crystal structure of the lysine-specific protease domain of Kgp to 1.6 Å resolution. The structure provides insights into the mechanism of substrate specificity and catalysis.
authors.affiliation: ACRF Rational Drug Discovery Centre, St. Vincent's Institute of Medical Research, Fitzroy, Victoria, 3065, Australia.
authors.forename: Michael A
authors.initials: MA
authors.lastname: Gorman
authors.forename: Christine A
authors.initials: CA
authors.lastname: Seers
authors.forename: Belinda J
authors.initials: BJ
authors.lastname: Michell
authors.forename: Susanne C
authors.initials: SC
authors.lastname: Feil
authors.forename: N Laila
authors.initials: NL
authors.lastname: Huq
authors.forename: Keith J
authors.initials: KJ
authors.lastname: Cross
authors.forename: Eric C
authors.initials: EC
authors.lastname: Reynolds
authors.forename: Michael W
authors.initials: MW
authors.lastname: Parker
mesh_terms: D018829:Adhesins, Bacterial / Q000737:chemistry* / Q000378:metabolism; D016866:Bacteroidaceae Infections / Q000382:microbiology* / Q000517:prevention & control; D018360:Crystallography, X-Ray; D003546:Cysteine Endopeptidases / Q000737:chemistry* / Q000378:metabolism; D000080867:Gingipain Cysteine Endopeptidases; D006801:Humans; D008958:Models, Molecular; D009909:Oral Health; D016966:Porphyromonas gingivalis / Q000737:chemistry* / Q000378:metabolism; D011487:Protein Conformation
chemical_list: D018829:Adhesins, Bacterial; D000080867:Gingipain Cysteine Endopeptidases; D003546:Cysteine Endopeptidases
keywords: Kgp; Porphyromonas gingivalis; gingipains; keystone pathogen; lysine specific protease; periodontitis