title: Microsatellite primers for Camissoniopsis cheiranthifolia (Onagraceae) and cross-amplification in related species.
abstract: OBJECTIVE
  We developed 24 nuclear microsatellite primers from an enriched genomic library for the Pacific coastal dune endemic Camissoniopsis cheiranthifolia to study the consequences of mating system differentiation, the genetics of species' range limits, and hybridization with its closest sister taxon, C. bistorta. •
  
  
  RESULTS
  Twenty-four primer pairs were developed and characterized in four populations of C. cheiranthifolia and one population of C. bistorta. We also tested eight additional taxa for cross-amplification. The average number of alleles per locus per species was 4.3 and 6.0, respectively. The number of loci that amplified and were variable within the eight related taxa ranged from six to 17. •
  
  
  CONCLUSIONS
  These markers will be useful in studying mating system evolution, the genetic structure of species' ranges, hybridization, and the provenance of material used for habitat restoration in C. cheiranthifolia, C. bistorta, and related species.
authors.affiliation: Department of Biology, Queen's University, Kingston, K7L 3N6 Ontario, Canada.
authors.forename: Adriana
authors.initials: A
authors.lastname: López-Villalobos
authors.affiliation: Department of Biology, University of Prince Edward Island, Charlottetown, C1A 4P3 Prince Edward Island, Canada.
authors.forename: Karen E
authors.initials: KE
authors.lastname: Samis
authors.affiliation: Department of Biology, Queen's University, Kingston, K7L 3N6 Ontario, Canada.
authors.forename: Christopher G
authors.initials: CG
authors.lastname: Eckert
keywords: Camissoniopsis bistorta; Camissoniopsis cheiranthifolia; hybridization; microsatellites; outcrossing; self-fertilization