title: Neuronal-like differentiation of bone marrow-derived mesenchymal stem cells induced by striatal extracts from a rat model of Parkinson's disease.
abstract: A rat model of Parkinson's disease was established by 6-hydroxydopamine injection into the medial forebrain bundle. Bone marrow-derived mesenchymal stem cells (BMSCs) were isolated from the femur and tibia, and were co-cultured with 10% and 60% lesioned or intact striatal extracts. The results showed that when exposed to lesioned striatal extracts, BMSCs developed bipolar or multi-polar morphologies, and there was an increase in the percentage of cells that expressed glial fibrillary acidic protein (GFAP), nestin and neuron-specific enolase (NSE). Moreover, the percentage of NSE-positive cells increased with increasing concentrations of lesioned striatal extracts. However, intact striatal extracts only increased the percentage of GFAP-positive cells. The findings suggest that striatal extracts from Parkinson's disease rats induce BMSCs to differentiate into neuronal-like cells in vitro.
authors.affiliation: Department of Neurology, Xuzhou Central Hospital, Xuzhou 221009, Jiangsu Province, China.
authors.forename: Xiaoling
authors.initials: X
authors.lastname: Qin
authors.affiliation: Department of Emergency, Dongying People's Hospital, Dongying 257091, Shandong Province, China.
authors.forename: Wang
authors.initials: W
authors.lastname: Han
authors.affiliation: Department of Neurology, Xuzhou Central Hospital, Xuzhou 221009, Jiangsu Province, China.
authors.forename: Zhigang
authors.initials: Z
authors.lastname: Yu
keywords: Parkinson’s disease; bone marrow-derived mesenchymal stem cell; glial fibrillary acidic protein; induced differentiation; nerve cell; nestin; neural regeneration; neural stem cell; neuron-specific enolase; regeneration; striatal extract