title: A Simple Route for Purifying Extracellular Poly(3-hydroxybutyrate)-depolymerase from Penicillium pinophilum.
abstract: This work proposes the purification of an active and efficient enzyme, extracellular poly(3-hydroxybutyrate) (PHB)-depolymerase, suitable for industrial applications. This is achieved by the application of an easy, fast, and cheap route, skipping the chromatography step. Chromatography with one or two columns is a common step in the purification procedure, which however renders the isolation of the enzyme a time consuming and an expensive process. A strain of the fungus Penicillium pinophilum (ATCC 9644) is used for the isolation of extracellular PHB-depolymerase. The molecular weight of the purified enzyme is about 35 kDa and is estimated by gel electrophoresis (SDS-PAGE, 12% polyacrylamide). The enzymatic activity of the isolated enzyme is determined to be 3.56-fold similar to that found by other researchers that have used chromatography for the isolation. The as-isolated enzyme disintegrates the poly(3-hydroxybutyrate) (PHB) films successfully, as it is demonstrated by the biodegradation test results provided here.
authors.affiliation: Department of Mechanical and Industrial Design Engineering, TEI of Western Macedonia, 50100 Kozani, Greece ; Department of Chemistry, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece.
authors.forename: Elpiniki
authors.identifier: 0000-0002-1375-2979
authors.initials: E
authors.lastname: Panagiotidou
authors.affiliation: Laboratory of Biotechnology, Department of Biological Applications and Technologies, University of Ioannina, 45100 Ioannina, Greece ; Laboratory of Biochemistry, Department of Biological Applications and Technologies, University of Ioannina, 45100 Ioannina, Greece.
authors.forename: Constantinos
authors.identifier: 0000-0003-4462-5072
authors.initials: C
authors.lastname: Konidaris
authors.affiliation: Department of Mechanical and Industrial Design Engineering, TEI of Western Macedonia, 50100 Kozani, Greece.
authors.forename: Apostolos
authors.identifier: 0000-0001-5642-1849
authors.initials: A
authors.lastname: Baklavaridis
authors.affiliation: Department of Mechanical and Industrial Design Engineering, TEI of Western Macedonia, 50100 Kozani, Greece ; Department of Chemical and Petroleum Engineering, United Arab Emirates University, Al Ain, UAE.
authors.forename: Ioannis
authors.identifier: 0000-0002-5969-8488
authors.initials: I
authors.lastname: Zuburtikudis
authors.affiliation: Department of Chemistry, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece.
authors.forename: Dimitris
authors.identifier: 0000-0003-2872-8426
authors.initials: D
authors.lastname: Achilias
authors.affiliation: Depertment of Agricultural Technology, School of Agriculture Technology, Food Technology and Nutrition, TEI of Western Macedonia, Terma Kontopoulou, 53100 Florina, Greece.
authors.forename: Paraskevi
authors.initials: P
authors.lastname: Mitlianga