title: Effect of apitherapy products against carbon tetrachloride-induced toxicity in Wistar rats.
abstract: The present paper aimed to evaluate the influence of apitherapy diet in Wistar rats with carbon tetrachloride induced hepatotoxicity, by the means of biochemical determinations and histopathological changes of liver, spleen, pancreas and testicular tissue. The experiment was carried out on six groups of male Wistar rats. Hepatic lesions were induced by intraperitoneal injection of carbon tetrachloride (dissolved in paraffin oil, 10% solution), 2 mL per 100 g, every two days, for two weeks. Hepatoprotection was achieved with two-apitherapy diet formulations (containing honey, pollen, propolis, Apilarnil, with/without royal jelly), that have been administered for six up to nine weeks. The biochemical results revealed that the two-apitherapy diet formulations had a positive effect improving the enzymatic, lipid, and protein profiles, coagulation, mineral parameters and also the bilirubin levels, after six weeks of treatment. The histopathological results demonstrated the benefit of the two-apitherapy diet formulations on reducing the toxicity of liver, spleen and pancreas in laboratory animals, after six and nine weeks, respectively. In conclusion, apitherapy products have a hepatoprotective effect in carbon tetrachloride-induced hepatopathy.
authors.affiliation: Discipline of Analytical Chemistry, Department of Pharmaceutical Sciences I, Faculty of Pharmacy, "Grigore T. Popa" University of Medicine and Pharmacy, Iassy, Romania; nelabibire@yahoo.com.
authors.forename: Călin Vasile
authors.initials: CV
authors.lastname: Andriţoiu
authors.forename: Vasile
authors.initials: V
authors.lastname: Andriţoiu
authors.forename: Magdalena
authors.initials: M
authors.lastname: Cuciureanu
authors.forename: Delia
authors.initials: D
authors.lastname: Nica-Badea
authors.forename: Nela
authors.initials: N
authors.lastname: Bibire
authors.forename: Marcel
authors.initials: M
authors.lastname: Popa
mesh_terms: D000818:Animals; D056705:Apitherapy; D001777:Blood Coagulation / Q000187:drug effects; D002251:Carbon Tetrachloride / Q000633:toxicity*; D007150:Immunohistochemistry; D008055:Lipids / Q000032:analysis; D008297:Male; D008903:Minerals / Q000032:analysis; D009928:Organ Specificity / Q000187:drug effects; D011506:Proteins / Q000032:analysis; D017208:Rats, Wistar
chemical_list: D008055:Lipids; D008903:Minerals; D011506:Proteins; D002251:Carbon Tetrachloride