title: Noninvasive allograft imaging of acute rejection: evaluation of (131)I-anti-CXCL10 mAb.
abstract: The purpose of this study was to investigate the use of iodine-131-labeled anti-CXCL10 mAb as tracer targeted at CXCL10 to detect acute rejection (AR) with mice model. Expression of CXCL10 was proved by RT-PCR, ELISA, and immunochemistry staining. All groups were submitted to whole-body autoradioimaging and ex vivo biodistribution studies after tail vein injection of (131)I-anti-CXCL10 mAb. The highest concentration/expression of CXCL10 was detected in allograft tissue compared with allograft treated with tacrolimus and isograft control. Tacrolimus could obviously inhibit the rejection of allograft. Allograft could be obviously imaged at all checking points, much clearer than the other two groups. The biodistribution results showed the highest uptake of radiotracer in allograft. T/NT (target/nontarget) ratio was 4.15 ± 0.25 at 72 h, apparently different from allograft treated with tacrolimus (2.29 ± 0.10), P < 0.05. These data suggest that CXCL10 is a promising target for early stage AR imaging and (131)I-CXCL10 mAb can successfully image AR and monitor the effect of immunosuppressant.
authors.affiliation: Key Laboratory for Experimental Teratology of the Ministry of Education and Biomedical Isotope Research Center, School of Medicine, Shandong University, 44# Wenhua Xi Road, Jinan, Shandong, 250012, People's Republic of China.
authors.forename: Dayan
authors.initials: D
authors.lastname: Cheng
authors.forename: Hukui
authors.initials: H
authors.lastname: Sun
authors.forename: Ting
authors.initials: T
authors.lastname: Liang
authors.forename: Chao
authors.initials: C
authors.lastname: Zhang
authors.forename: Jing
authors.initials: J
authors.lastname: Song
authors.forename: Guihua
authors.initials: G
authors.lastname: Hou
mesh_terms: D064591:Allografts / Q000000981:diagnostic imaging* / Q000378:metabolism*; D000818:Animals; D001345:Autoradiography / Q000379:methods; D054357:Chemokine CXCL10 / Q000096:biosynthesis*; D005260:Female; D006084:Graft Rejection / Q000000981:diagnostic imaging* / Q000378:metabolism*; D007457:Iodine Radioisotopes; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D011859:Radiography
chemical_list: D054357:Chemokine CXCL10; C485433:Cxcl10 protein, mouse; D007457:Iodine Radioisotopes