title: Simultaneous quantitative analysis of nine triterpenoid saponins for the quality control of Stauntonia obovatifoliola Hayata subsp. intermedia stems.
abstract: Stauntonia obovatifoliola Hayata subsp. intermedia is used in China to treat rheumatic arthralgia, hernia pain, and traumatic pain. An accurate and reliable method based on high-performance liquid chromatography with diode array detection has been developed and validated for the quantitative determination of nine triterpenoid saponins in this herb. By using a Kromasil 100-5 C18 column (250 mm × 4.6 mm, 5 μm), nine analytes were separated by gradient elution over a running time of 45.0 min. All standard calibration curves demonstrated satisfactory linearity (R(2) ≥ 0.9995) within a relatively wide range. The precision was evaluated by intra- and interday tests, which revealed relative standard deviation values within the ranges of 0.20-2.83 and 0.51-2.79%, respectively. The recoveries for the nine target compounds were between 84.6 and 103% with relative standard deviation values less than 2.67%. The samples were also analyzed on a linear trap quadrupole Orbitrap Velos Pro mass spectrometer equipped with an electrospray ionization source in negative mode to confirm the quantification results. In conclusion, the present high-performance liquid chromatography with diode array detection method could serve as an accurate and reliable method for the quality evaluation of Stauntonia obovatifoliola Hayata subsp. intermedia stems.
authors.affiliation: School of Traditional Chinese Medicine, Capital Medical University, Beijing, China.
authors.forename: Xuran
authors.initials: X
authors.lastname: Lu
authors.forename: Feng
authors.initials: F
authors.lastname: Qiu
authors.forename: Xueqiang
authors.initials: X
authors.lastname: Pan
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.forename: Manyuan
authors.initials: M
authors.lastname: Wang
authors.forename: Muxin
authors.initials: M
authors.lastname: Gong
mesh_terms: D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D008968:Molecular Conformation; D018547:Plant Stems / Q000737:chemistry*; D011786:Quality Control; D012503:Saponins / Q000032:analysis*; D014315:Triterpenes / Q000032:analysis*
chemical_list: D004365:Drugs, Chinese Herbal; D012503:Saponins; D014315:Triterpenes
keywords: Quality control; Stauntonia obovatifoliola Hayata subsp. intermedia; Triterpenoid saponins