title: Nitrosospira lacus sp. nov., a psychrotolerant, ammonia-oxidizing bacterium from sandy lake sediment.
abstract: A Gram-negative, spiral-shaped, chemolithotrophic, ammonia-oxidizing bacterium, designated APG3(T), was isolated into pure culture from sandy lake sediment collected from Green Lake, Seattle, WA, USA. Phylogenetic analyses based on the 16S rRNA gene sequence showed that strain APG3(T) belongs to cluster 0 of the genus Nitrosospira, which is presently not represented by described species, with Nitrosospira multiformis (cluster 3) as the closest species with a validly published name (identity of 98.6 % to the type strain). Strain APG3(T) grew at 4 °C but could not grow at 35 °C, indicating that this bacterium is psychrotolerant. Remarkably, the strain was able to grow over a wide range of pH (pH 5-9), which was greater than the pH range of any studied ammonia-oxidizing bacteria in pure culture. The DNA G+C content of the APG3(T) genome is 53.5 %, which is similar to that of Nitrosospira multiformis ATCC 25196(T) (53.9 %) but higher than that of Nitrosomonas europaea ATCC 19718 (50.7 %) and Nitrosomonas eutropha C71 (48.5 %). The average nucleotide identity (ANI) calculated for the genomes of strain APG3(T) and Nitrosospira multiformis ATCC 25196(T) was 75.45 %, significantly lower than the value of 95 % ANI that corresponds to the 70 % species-level cut-off based on DNA-DNA hybridization. Overall polyphasic taxonomy study indicated that strain APG3(T) represents a novel species in the genus Nitrosospira, for which the name Nitrosospira lacus sp. nov. is proposed (type strain APG3(T) = NCIMB 14869(T) = LMG 27536(T) = ATCC BAA-2542(T)).
authors.affiliation: Department of Civil and Environmental Engineering, University of Washington, Seattle, WA, USA.|Department of Marine and Ecological Sciences, Florida Gulf Coast University, Fort Myers, FL, USA.
authors.forename: Hidetoshi
authors.initials: H
authors.lastname: Urakawa
authors.affiliation: Department of Marine and Ecological Sciences, Florida Gulf Coast University, Fort Myers, FL, USA.
authors.forename: Juan C
authors.initials: JC
authors.lastname: Garcia
authors.affiliation: Department of Biotechnology, Chemistry and Environmental Engineering, Aalborg University, Aalborg, Denmark.
authors.forename: Jeppe L
authors.initials: JL
authors.lastname: Nielsen
authors.affiliation: Department of Biotechnology, Chemistry and Environmental Engineering, Aalborg University, Aalborg, Denmark.
authors.forename: Vang Q
authors.initials: VQ
authors.lastname: Le
authors.affiliation: Department of Biological Sciences, University of Alberta, Edmonton, AB, Canada.
authors.forename: Jessica A
authors.initials: JA
authors.lastname: Kozlowski
authors.affiliation: Department of Biological Sciences, University of Alberta, Edmonton, AB, Canada.
authors.forename: Lisa Y
authors.initials: LY
authors.lastname: Stein
authors.affiliation: Department of Biological Sciences, University of North Carolina, Charlotte, NC, USA.
authors.forename: Chee Kent
authors.initials: CK
authors.lastname: Lim
authors.affiliation: Department of Microbiology and Biotechnology, Biocenter Klein Flottbek, University of Hamburg, Hamburg, Germany.
authors.forename: Andreas
authors.initials: A
authors.lastname: Pommerening-Röser
authors.affiliation: Department of Civil and Environmental Engineering, University of Washington, Seattle, WA, USA.
authors.forename: Willm
authors.initials: W
authors.lastname: Martens-Habbena
authors.affiliation: Department of Civil and Environmental Engineering, University of Washington, Seattle, WA, USA.
authors.forename: David A
authors.initials: DA
authors.lastname: Stahl
authors.affiliation: Institute of Marine Microbes & Ecospheres and State Key Laboratory of Marine Environmental Science, Xiamen University, Xiamen, PR China.|Department of Biological Sciences, University of North Carolina, Charlotte, NC, USA.
authors.forename: Martin G
authors.initials: MG
authors.lastname: Klotz
mesh_terms: D000641:Ammonia / Q000378:metabolism*; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D060106:Lakes / Q000382:microbiology*; D008969:Molecular Sequence Data; D043369:Nitrosomonadaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S; D000641:Ammonia