title: Real-time UV-visible spectroscopy analysis of purple membrane-polyacrylamide film formation taking into account Fano line shapes and scattering.
abstract: We theoretically and experimentally analyze the formation of thick Purple Membrane (PM) polyacrylamide (PA) films by means of optical spectroscopy by considering the absorption of bacteriorhodopsin and scattering. We have applied semiclassical quantum mechanical techniques for the calculation of absorption spectra by taking into account the Fano effects on the ground state of bacteriorhodopsin. A model of the formation of PM-polyacrylamide films has been proposed based on the growth of polymeric chains around purple membrane. Experimentally, the temporal evolution of the polymerization process of acrylamide has been studied as function of the pH solution, obtaining a good correspondence to the proposed model. Thus, due to the formation of intermediate bacteriorhodopsin-doped nanogel, by controlling the polymerization process, an alternative methodology for the synthesis of bacteriorhodopsin-doped nanogels can be provided.
authors.affiliation: Departamento de Ciencia de Materiales, Óptica y Tecnología Electrónica, Universidad Miguel Hernández, Elx (Alicante), Spain.
authors.forename: María
authors.initials: M
authors.lastname: Gomariz
authors.affiliation: Departamento de Ciencia de Materiales, Óptica y Tecnología Electrónica, Universidad Miguel Hernández, Elx (Alicante), Spain.
authors.forename: Salvador
authors.initials: S
authors.lastname: Blaya
authors.affiliation: Departamento de Ciencia de Materiales, Óptica y Tecnología Electrónica, Universidad Miguel Hernández, Elx (Alicante), Spain.
authors.forename: Pablo
authors.initials: P
authors.lastname: Acebal
authors.affiliation: Departamento de Ciencia de Materiales, Óptica y Tecnología Electrónica, Universidad Miguel Hernández, Elx (Alicante), Spain.
authors.forename: Luis
authors.initials: L
authors.lastname: Carretero
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry*; D001436:Bacteriorhodopsins / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D011487:Protein Conformation; D018717:Purple Membrane / Q000737:chemistry*; D013057:Spectrum Analysis; D000099166:Polyacrylamides
chemical_list: D000180:Acrylic Resins; D001436:Bacteriorhodopsins; D000099166:Polyacrylamides