title: The role of DNA polymerase alpha in the control of mutagenesis inSaccharomyces cerevisiaecells starved for nutrients.
abstract: In nature, micro organisms experience numerous environmental stresses and generally grow poorly most of the time. In the last two decades it has become evident that mutations arise not only in actively dividing cells but also in non-replicating or slowly replicating cells starved for nutrients. In yeast, precise base selection and proofreading by replicative DNA polymerases δ and ε keep starvation-associated mutagenesis (SAM) at basal levels. Less is known about the role of replicative DNA polymerase α (Pol α). Here we provide evidence that Pol α is involved in the control of SAM in yeast cells starved for adenine by participation in sporadic replication and/or DNA repair under these conditions.
authors.affiliation: Associate Professor, Dipartimento di Biologia Cellulare e Ambientale, Università di Perugia, Via Elce di Sotto, 06100, Perugia, Italy, babudri@unipg.it.
authors.forename: Nora
authors.initials: N
authors.lastname: Babudri
authors.affiliation: Researcher, Dipartimento di Genetica e Microbiologia, Università di Pavia, Via Ferrata 1, 27100, Pavia; Dipartimento di Biologia Cellulare e Ambientale, Università di Perugia, Via Elce di Sotto, 06100, Perugia, Italy, alessandro.achilli@unipg.it.
authors.forename: Alessandro
authors.initials: A
authors.lastname: Achilli
authors.affiliation: Degree in Biological Science, Dipartimento di Genetica e Microbiologia, Università di Pavia, Via Ferrata 1, 27100, Pavia, Italy, chiaramartinelli@hotmail.it.
authors.forename: Chiara
authors.initials: C
authors.lastname: Martinelli
authors.affiliation: Laboratory Supervisor, Eppley Institute for Research in Cancer, University of Nebraska Medical Center, 68198-6805, Omaha, NE, U.S.A, eamoore@unmc.edu.
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Moore
authors.affiliation: PhD, Dipartimento di Biologia Cellulare e Ambientale, Università di Perugia, Via Elce di Sotto, 06100, Perugia, Italy, lancioni@kataweb.com.
authors.forename: Hovirag
authors.initials: H
authors.lastname: Lancioni
authors.affiliation: Associate Professor, Department of Biochemistry and Molecular Biology, Department of Microbiology and Pathology, and Eppley Institute for Research in Cancer, University of Nebraska Medical Center, 68198-6805, Omaha, NE, U.S.A, ypavlov@unmc.edu.
authors.forename: Youri I
authors.initials: YI
authors.lastname: Pavlov
keywords: DNA Polymerase α; Saccharomyces cerevisiae; starvation-associated mutagenesis