title: Recombinant rabies virus expressing IFNα1 enhanced immune responses resulting in its attenuation and stronger immunogenicity.
abstract: Several studies have shown that type 1 interferons (IFNs) exert multiple biological effects on both innate and adaptive immune responses. Here, we investigated the pathogenicity and immunogenicity of recombinant rabies virus (RABV) expressing canine interferon α1 (rHEP-CaIFNα1). It was shown that Kun Ming (KM) mice that received a single intramuscular immunization with rHEP-CaIFNα1 had an earlier increase and a higher level of virus-neutralizing antibody titers compared with immunization of the parent HEP-Flury. A challenge experiment further confirmed that more mice that were immunized with rHEP-CaIFNα1 survived compared with mice immunized with the parent virus. Quantitative real-time PCR indicated that rHEP-CaIFNα1 induced a stronger innate immune response, especially the type 1 IFN response. Flow cytometry was conducted to show that rHEP-CaIFNα1 recruited more activated B cells in lymph nodes and CD8 T cells in the peripheral blood, which is beneficial to achieve virus clearance in the early infective stage.
authors.affiliation: College of Veterinary Medicine, South China Agricultural University, Guangzhou, China.
authors.forename: Yifei
authors.initials: Y
authors.lastname: Wang
authors.affiliation: College of Veterinary Medicine, South China Agricultural University, Guangzhou, China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Tian
authors.affiliation: College of Veterinary Medicine, South China Agricultural University, Guangzhou, China.
authors.forename: Xiaojuan
authors.initials: X
authors.lastname: Xu
authors.affiliation: College of Veterinary Medicine, South China Agricultural University, Guangzhou, China.
authors.forename: Xianfeng
authors.initials: X
authors.lastname: Yang
authors.affiliation: College of Veterinary Medicine, South China Agricultural University, Guangzhou, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Luo
authors.affiliation: College of Veterinary Medicine, South China Agricultural University, Guangzhou, China.
authors.forename: Weiyu
authors.initials: W
authors.lastname: Mo
authors.affiliation: College of Veterinary Medicine, South China Agricultural University, Guangzhou, China.
authors.forename: Jiaojiao
authors.initials: J
authors.lastname: Peng
authors.affiliation: The First Affiliated Hospital, Guangzhou Medical University, Guangzhou, China.
authors.forename: Xuefeng
authors.initials: X
authors.lastname: Niu
authors.affiliation: College of Veterinary Medicine, South China Agricultural University, Guangzhou, China.
authors.forename: Yongwen
authors.initials: Y
authors.lastname: Luo
authors.affiliation: College of Veterinary Medicine, South China Agricultural University, Guangzhou, China. Electronic address: xfguo@scau.edu.cn.
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Guo
mesh_terms: D000818:Animals; D001402:B-Lymphocytes; D006224:Cricetinae; D004285:Dogs; D015967:Gene Expression Regulation, Viral / Q000276:immunology*; D005818:Genetic Engineering; D007113:Immunity, Innate; D016898:Interferon-alpha / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D012333:RNA, Messenger; D012367:RNA, Viral; D011818:Rabies / Q000517:prevention & control / Q000821:virology; D011819:Rabies Vaccines / Q000276:immunology*; D011820:Rabies virus / Q000235:genetics* / Q000276:immunology / Q000472:pathogenicity*; D013601:T-Lymphocytes; D014613:Vaccines, Attenuated; D014776:Virus Cultivation; D014779:Virus Replication
chemical_list: D016898:Interferon-alpha; D012333:RNA, Messenger; D012367:RNA, Viral; D011819:Rabies Vaccines; D014613:Vaccines, Attenuated
keywords: Interferon; Rabies virus; Vaccine