title: When functionalization of PLA surfaces meets Thiol-Yne photochemistry: case study with antibacterial polyaspartamide derivatives.
abstract: In this work we wish to report on the covalent functionalization of polylactide (PLA) surfaces by photoradical thiol-yne to yield antibacterial surfaces. At first, hydrophilic and hydrophobic thiol fluorescent probes are synthesized and used to study and optimize the conditions of ligation on alkyne-PLA surfaces. In a second part, a new antibacterial polyaspartamide copolymer is covalently grafted. The covalent surface modification and the density of surface functionalization are evaluated by SEC and XPS analyses. No degradation of PLA chains is observed, whereas covalent grafting is confirmed by the presence of S2p and N1s signals. Antiadherence and antibiofilm activities are assessed against four bacterial strains, including Gram-negative and Gram-positive bacteria. A strong activity is observed with adherence reduction factors superior to 99.98% and biofilm formation decreased by 80%. Finally, in vitro cytocompatibility tests of the antibacterial surfaces are performed with L929 murine fibroblasts and show cell viability without promoting proliferation.
authors.affiliation: Institut des Biomolécules Max Mousseron (CNRS UMR 5247), Département des Biopolymères Artificiels, UFR Pharmacie - Université Montpellier I, Université Montpellier 2-15, Avenue Charles Flahaut, 34093 Montpellier, France.
authors.forename: Carla
authors.initials: C
authors.lastname: Sardo
authors.forename: Benjamin
authors.initials: B
authors.lastname: Nottelet
authors.forename: Daniela
authors.initials: D
authors.lastname: Triolo
authors.forename: Gaetano
authors.initials: G
authors.lastname: Giammona
authors.forename: Xavier
authors.initials: X
authors.lastname: Garric
authors.forename: Jean-Philippe
authors.initials: JP
authors.lastname: Lavigne
authors.forename: Gennara
authors.initials: G
authors.lastname: Cavallaro
authors.forename: Jean
authors.initials: J
authors.lastname: Coudane
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000378:metabolism; D002460:Cell Line; D051379:Mice; D010455:Peptides / Q000737:chemistry* / Q000378:metabolism; D010777:Photochemistry / Q000379:methods*; D011091:Polyesters / Q000737:chemistry* / Q000378:metabolism; D013438:Sulfhydryl Compounds / Q000737:chemistry* / Q000378:metabolism; D013499:Surface Properties
chemical_list: D000900:Anti-Bacterial Agents; D010455:Peptides; D011091:Polyesters; D013438:Sulfhydryl Compounds; C017645:polyaspartate; C033616:poly(lactide)