title: Osteopontin, osteocalcin and OB-cadherin expression in Synthetic nanohydroxyapatite vs bovine hydroxyapatite cultured Osteoblastic-like cells.
abstract: Calcium phosphate ceramics have been applied in bone replacement for several decades due to their excellent biocompatibility, bioactivity, osteo-conductivity and mechanical strength. Several studies have demonstrated that porous hydroxyapatite (HA) is an excellent scaffold for osteogenic proliferation and differentiation of the osteoprogenitor cells. However, different methods of synthesis and production of HA ceramic-based materials may have considerable effect on the physical and biological properties. In the present work, two hydroxyapatite-based materials, a natural hydroxyapatite ceramic of bovine origin and a synthetic nano-cristalline hydroxyapatite were tested in vitro with MG63 cell line. The results displayed that both the materials demonstrated a good biocompatibility. The immunocytochemical stain revealed a different positivity of the osteogenic markers between the cultures with the biomaterials, and the control culture. Western blot data confirmed the immunocytochemical stain. Both the materials tested in the present study demonstrated a good biocompatibility with the osteoblastic cells allowing, at the same time, the osteogenic differentiation, and they may be useful in clinical use.
authors.affiliation: Department of Clinical, Specialistic and Stomatological Sciences, Marche Polytechnic University, Ancona, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Santarelli
authors.affiliation: Department of Clinical, Specialistic and Stomatological Sciences, Marche Polytechnic University, Ancona, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Mascitti
authors.affiliation: Department of Clinical, Specialistic and Stomatological Sciences, Marche Polytechnic University, Ancona, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Orsini
authors.affiliation: Department of Clinical, Specialistic and Stomatological Sciences, Marche Polytechnic University, Ancona, Italy.
authors.forename: L
authors.initials: L
authors.lastname: Memè
authors.affiliation: Department of Pathologic Anatomy and Histopathology, Marche Polytechnic University, Ancona, Italy.
authors.forename: R
authors.initials: R
authors.lastname: Rocchetti
authors.affiliation: Department of Clinical, Specialistic and Stomatological Sciences, Marche Polytechnic University, Ancona, Italy.
authors.forename: P
authors.initials: P
authors.lastname: Tiriduzzi
authors.affiliation: Department of Clinical, Specialistic and Stomatological Sciences, Marche Polytechnic University, Ancona, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Sampalmieri
authors.affiliation: Department of Clinical, Specialistic and Stomatological Sciences, Marche Polytechnic University, Ancona, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Putignano
authors.affiliation: Department of Clinical, Specialistic and Stomatological Sciences, Marche Polytechnic University, Ancona, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Procaccini
authors.affiliation: Department of Clinical and Experimental Medicine, Foggia University, Foggia, Italy.
authors.forename: L
authors.initials: L
authors.lastname: Lo Muzio
authors.affiliation: Department of Clinical, Specialistic and Stomatological Sciences, Marche Polytechnic University, Ancona, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Bambini
mesh_terms: D000818:Animals; D015820:Cadherins / Q000096:biosynthesis*; D002417:Cattle; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D002516:Ceramics / Q000138:chemical synthesis / Q000494:pharmacology; D017886:Durapatite / Q000138:chemical synthesis / Q000494:pharmacology*; D008422:Materials Testing; D049329:Nanostructures; D010006:Osteoblasts / Q000166:cytology / Q000378:metabolism*; D015675:Osteocalcin / Q000096:biosynthesis*; D053495:Osteopontin / Q000096:biosynthesis*
chemical_list: D015820:Cadherins; D015675:Osteocalcin; D053495:Osteopontin; C086960:osteoblast cadherin; D017886:Durapatite