title: Atomic force microscopy fishing and mass spectrometry identification of gp120 on immobilized aptamers.
abstract: Atomic force microscopy (AFM) was applied to carry out direct and label-free detection of gp120 human immunodeficiency virus type 1 envelope glycoprotein as a target protein. This approach was based on the AFM fishing of gp120 from the analyte solution using anti-gp120 aptamers immobilized on the AFM chip to count gp120/aptamer complexes that were formed on the chip surface. The comparison of image contrasts of fished gp120 against the background of immobilized aptamers and anti-gp120 antibodies on the AFM images was conducted. It was shown that an image contrast of the protein/aptamer complexes was two-fold higher than the contrast of the protein/antibody complexes. Mass spectrometry identification provided an additional confirmation of the target protein presence on the AFM chips after biospecific fishing to avoid any artifacts.
authors.affiliation: Department of Personalized Medicine, Orekhovich Institute of Biomedical Chemistry of the Russian Academy of Medical Sciences, Moscow, Russia.
authors.forename: Yuri D
authors.initials: YD
authors.lastname: Ivanov
authors.affiliation: Department of Personalized Medicine, Orekhovich Institute of Biomedical Chemistry of the Russian Academy of Medical Sciences, Moscow, Russia.
authors.forename: Natalia S
authors.initials: NS
authors.lastname: Bukharina
authors.affiliation: Department of Personalized Medicine, Orekhovich Institute of Biomedical Chemistry of the Russian Academy of Medical Sciences, Moscow, Russia.
authors.forename: Tatyana O
authors.initials: TO
authors.lastname: Pleshakova
authors.affiliation: Department of Personalized Medicine, Orekhovich Institute of Biomedical Chemistry of the Russian Academy of Medical Sciences, Moscow, Russia.
authors.forename: Pavel A
authors.initials: PA
authors.lastname: Frantsuzov
authors.affiliation: Department of Personalized Medicine, Orekhovich Institute of Biomedical Chemistry of the Russian Academy of Medical Sciences, Moscow, Russia.
authors.forename: Elena Yu
authors.initials: EY
authors.lastname: Andreeva
authors.affiliation: Department of Personalized Medicine, Orekhovich Institute of Biomedical Chemistry of the Russian Academy of Medical Sciences, Moscow, Russia ; PostgenTech Ltd., Moscow, Russia.
authors.forename: Anna L
authors.initials: AL
authors.lastname: Kaysheva
authors.affiliation: Department of Personalized Medicine, Orekhovich Institute of Biomedical Chemistry of the Russian Academy of Medical Sciences, Moscow, Russia.
authors.forename: Victor G
authors.initials: VG
authors.lastname: Zgoda
authors.affiliation: Department of Personalized Medicine, Orekhovich Institute of Biomedical Chemistry of the Russian Academy of Medical Sciences, Moscow, Russia.
authors.forename: Alexander A
authors.initials: AA
authors.lastname: Izotov
authors.affiliation: Department of Personalized Medicine, Orekhovich Institute of Biomedical Chemistry of the Russian Academy of Medical Sciences, Moscow, Russia.
authors.forename: Tatyana I
authors.initials: TI
authors.lastname: Pavlova
authors.affiliation: Department of Personalized Medicine, Orekhovich Institute of Biomedical Chemistry of the Russian Academy of Medical Sciences, Moscow, Russia.
authors.forename: Vadim S
authors.initials: VS
authors.lastname: Ziborov
authors.affiliation: Department of Personalized Medicine, Orekhovich Institute of Biomedical Chemistry of the Russian Academy of Medical Sciences, Moscow, Russia.
authors.forename: Sergey P
authors.initials: SP
authors.lastname: Radko
authors.affiliation: Department of Personalized Medicine, Orekhovich Institute of Biomedical Chemistry of the Russian Academy of Medical Sciences, Moscow, Russia.
authors.forename: Sergei A
authors.initials: SA
authors.lastname: Moshkovskii
authors.affiliation: Department of Personalized Medicine, Orekhovich Institute of Biomedical Chemistry of the Russian Academy of Medical Sciences, Moscow, Russia.
authors.forename: Alexander I
authors.initials: AI
authors.lastname: Archakov
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry*; D015699:HIV Envelope Protein gp120 / Q000032:analysis* / Q000737:chemistry*; D061925:Immobilized Nucleic Acids / Q000737:chemistry*; D013058:Mass Spectrometry / Q000379:methods*; D018625:Microscopy, Atomic Force / Q000379:methods*; D015336:Molecular Probe Techniques
chemical_list: D052157:Aptamers, Nucleotide; D015699:HIV Envelope Protein gp120; D061925:Immobilized Nucleic Acids
keywords: aptamer; atomic force microscopy; gp120 HIV-1 envelope glycoprotein; mass spectrometry