title: Application of 3-d echocardiography and gated micro-computed tomography to assess cardiomyopathy in a mouse model of duchenne muscular dystrophy.
abstract: The purpose of this study was to measure changes in cardiac function as cardiomyopathy progresses in a mouse model of Duchenne muscular dystrophy using 3-D ECG-gated echocardiography. This study is the first to correlate cardiac volumes acquired using 3-D echocardiography with those acquired using retrospectively gated micro-computed tomography (CT). Both were further compared with standard M-mode echocardiography and histologic analyses. We found that although each modality measures a decrease in cardiac function as disease progresses in mdx/utrn(-/-) mice (n = 5) compared with healthy C57BL/6 mice (n = 8), 3-D echocardiography has higher agreement with gold-standard measurements acquired by gated micro-CT, with little standard deviation between measurements. M-Mode echocardiography measurements, in comparison, exhibit considerably greater variability and user bias. Given the radiation dose associated with micro-CT and the geometric assumptions made in M-mode echocardiography to calculate ventricular volume, we suggest that use of 3-D echocardiography has important advantages that may allow for the measurement of early disease changes that occur before overt cardiomyopathy.
authors.affiliation: Imaging Program, Lawson Health Research Institute, London, Ontario, Canada; Department of Medical Biophysics, Western University, London, Ontario, Canada.
authors.forename: Andrew B
authors.initials: AB
authors.lastname: Bondoc
authors.affiliation: Imaging Research Laboratories, Robarts Research Institute, London, Ontario, Canada.
authors.forename: Sarah
authors.initials: S
authors.lastname: Detombe
authors.affiliation: Imaging Research Laboratories, Robarts Research Institute, London, Ontario, Canada.
authors.forename: Joy
authors.initials: J
authors.lastname: Dunmore-Buyze
authors.affiliation: Imaging Program, Lawson Health Research Institute, London, Ontario, Canada; Department of Anatomy & Cell Biology, Western University, London, Ontario, Canada.
authors.forename: Kelly M
authors.initials: KM
authors.lastname: Gutpell
authors.affiliation: Imaging Program, Lawson Health Research Institute, London, Ontario, Canada; Department of Medical Biophysics, Western University, London, Ontario, Canada.
authors.forename: Linshan
authors.initials: L
authors.lastname: Liu
authors.affiliation: Imaging Program, Lawson Health Research Institute, London, Ontario, Canada.
authors.forename: Amanda
authors.initials: A
authors.lastname: Kaszuba
authors.affiliation: Imaging Program, Lawson Health Research Institute, London, Ontario, Canada.
authors.forename: Seongryoung
authors.initials: S
authors.lastname: Han
authors.affiliation: Imaging Program, Lawson Health Research Institute, London, Ontario, Canada.
authors.forename: Rebecca
authors.initials: R
authors.lastname: McGirr
authors.affiliation: Imaging Program, Lawson Health Research Institute, London, Ontario, Canada; Imaging Research Laboratories, Robarts Research Institute, London, Ontario, Canada.
authors.forename: Jennifer
authors.initials: J
authors.lastname: Hadway
authors.affiliation: Department of Medical Biophysics, Western University, London, Ontario, Canada; Imaging Research Laboratories, Robarts Research Institute, London, Ontario, Canada.
authors.forename: Maria
authors.initials: M
authors.lastname: Drangova
authors.affiliation: Imaging Program, Lawson Health Research Institute, London, Ontario, Canada; Department of Medical Biophysics, Western University, London, Ontario, Canada; Department of Anatomy & Cell Biology, Western University, London, Ontario, Canada. Electronic address: lhoffman@lawsonimaging.ca.
authors.forename: Lisa M
authors.initials: LM
authors.lastname: Hoffman
mesh_terms: D000818:Animals; D055424:Cardiac-Gated Imaging Techniques / Q000379:methods*; D019560:Echocardiography, Three-Dimensional / Q000379:methods*; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D020388:Muscular Dystrophy, Duchenne; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014057:Tomography, X-Ray Computed / Q000379:methods*
keywords: 3-D echocardiography; Dilated cardiomyopathy; Duchenne muscular dystrophy; Gated micro-computed tomography; M-Mode echocardiography; mdx/utrn(–/–) mice