title: Evenly distributed thin-film Ag coating on stainless plate by tricomponent Ag/silicate/PU with antimicrobial and biocompatible properties.
abstract: A tricomponent nanohybrid dispersion in water comprising silver nanoparticles (AgNP), nanometer-thick silicate platelets (NSP), and water-based polyurethane (PU) was developed for surface coating on orthopedic metal plates. The previously developed AgNP-on-NSP nanohybrid was homogeneously blended into a selected waterborne PU dispersion at varied weight ratios from 1/0.1 to 1/10 (w/w). PU was used to adhere the Ag nanohybrid to the metal surface. The resultant dispersions were analyzed and found to contain AgNP 2-18 nm in diameter and characterized by using UV absorption and TEM micrograph. The subsequent coating of AgNP/NSP-PU dispersion generated a film of 1.5 μm thickness on the metal plate surface, further characterized by an energy dispersive spectroscope (EDS) to show the homogeneous distribution of Ag, Si, and C elements on the metal plates. The surface antimicrobial efficacy was proven for the coating composition of AgNP/NSP to PU ranging from 1/1 to 1/5 by weight ratio but irrelevant to the thickness of the coated materials. The metal plate coated with the high Ag content at 1/1 (w/w) ratio was shown to have very low cytotoxicity toward the contacted mammal fibroblasts. Overall, the optimized tricomponent Ag/silicate/PU in water dispersion from 1/2 to 1/3 (w/w) could generate a stable film on a metal surface exhibiting both antimicrobial and biocompatible properties. The facile coating technique of the AgNP/NSP in waterborne PU is proven to be viable for fabricating infection- and cytotoxicity-free medical devices.
authors.affiliation: Department of Orthopedics, National Taiwan University Hospital and National Taiwan University College of Medicine , Taipei 10002, Taiwan.
authors.forename: Yi-Hsiu
authors.initials: YH
authors.lastname: Huang
authors.forename: Mark Hung-Chih
authors.initials: MH
authors.lastname: Chen
authors.forename: Bing-Heng
authors.initials: BH
authors.lastname: Lee
authors.forename: Kuo-Huang
authors.initials: KH
authors.lastname: Hsieh
authors.forename: Yuan-Kun
authors.initials: YK
authors.lastname: Tu
authors.forename: Jiang-Jen
authors.initials: JJ
authors.lastname: Lin
authors.forename: Chih-Hao
authors.initials: CH
authors.lastname: Chang
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000737:chemistry* / Q000494:pharmacology; D041702:BALB 3T3 Cells; D002470:Cell Survival / Q000187:drug effects; D020099:Coated Materials, Biocompatible / Q000737:chemistry* / Q000494:pharmacology; D053768:Metal Nanoparticles / Q000737:chemistry; D051379:Mice; D018625:Microscopy, Atomic Force; D008855:Microscopy, Electron, Scanning; D011140:Polyurethanes / Q000737:chemistry*; D017640:Silicates / Q000737:chemistry*; D012834:Silver / Q000737:chemistry*; D013052:Spectrometry, X-Ray Emission; D013193:Stainless Steel / Q000737:chemistry*; D013211:Staphylococcus aureus / Q000187:drug effects; D013499:Surface Properties
chemical_list: D000890:Anti-Infective Agents; D020099:Coated Materials, Biocompatible; D011140:Polyurethanes; D017640:Silicates; D013193:Stainless Steel; D012834:Silver
keywords: antibacterial; medical device; nanosilicate platelets (NSP); silver nanoparticles (AgNP); stainless plate; surface treatment; waterborne polyurethane (PU)