title: Synthesis aided structural determination of amyloid-β(1-15) glycopeptides, new biomarkers for Alzheimer's disease.
abstract: Unique tyrosine glycosylated amyloid-β(1-15) glycopeptides were synthesized with well-defined stereochemistry at the glycosidic linkages. Aided by these glycopeptides and tandem mass spectrometry analysis, the naturally existing amyloid-β glycopeptides, isolated from Alzheimer's disease patients, were determined to contain an α-linked N-acetyl galactosamine at the modified tyrosine 10 residue. Glycosylation can significantly impact the properties of amyloid-β as the glycopeptide has much lower affinity for Cu(+) ions.
authors.affiliation: Department of Chemistry, Michigan State University, 578 S. Shaw Lane, East Lansing, Michigan 48824, USA. xuefei@chemistry.msu.edu.
authors.forename: Peng
authors.initials: P
authors.lastname: Wang
authors.forename: Jonas
authors.initials: J
authors.lastname: Nilsson
authors.forename: Gunnar
authors.initials: G
authors.lastname: Brinkmalm
authors.forename: Göran
authors.initials: G
authors.lastname: Larson
authors.forename: Xuefei
authors.initials: X
authors.lastname: Huang
mesh_terms: D000544:Alzheimer Disease; D016229:Amyloid beta-Peptides / Q000138:chemical synthesis / Q000737:chemistry*; D015415:Biomarkers / Q000737:chemistry; D006801:Humans; D015394:Molecular Structure; D010446:Peptide Fragments / Q000138:chemical synthesis / Q000737:chemistry*; D053719:Tandem Mass Spectrometry
chemical_list: D016229:Amyloid beta-Peptides; D015415:Biomarkers; D010446:Peptide Fragments; C480626:amyloid beta-protein (1-15)