title: Thiol-assisted one-pot synthesis of peptide/protein C-terminal thioacids from peptide/protein hydrazides at neutral conditions.
abstract: An efficient thiol-assisted one-pot synthesis of peptide/protein C-terminal thioacids was achieved by using peptide/protein hydrazides precursors at neutral pH and room temperature (about 20 °C). The transformation from hydrazides to thioacids was shown to be efficient for different C-terminal amino acids and was racemization-free. The in situ formed peptide-thioacids were further used for protein chemical synthesis and site-specific labelling successfully.
authors.affiliation: School of Medical Engineering, School of Chemistry and Chemical Engineering, Hefei University of Technology, Hefei, Anhui 230009, China. lym2007@mail.ustc.edu.cn ymli@hfut.edu.cn.
authors.forename: Chenchen
authors.initials: C
authors.lastname: Chen
authors.forename: Yichao
authors.initials: Y
authors.lastname: Huang
authors.forename: Ling
authors.initials: L
authors.lastname: Xu
authors.forename: Yong
authors.initials: Y
authors.lastname: Zheng
authors.forename: Huajian
authors.initials: H
authors.lastname: Xu
authors.forename: Qingxiang
authors.initials: Q
authors.lastname: Guo
authors.forename: Changlin
authors.initials: C
authors.lastname: Tian
authors.forename: Yiming
authors.initials: Y
authors.lastname: Li
authors.forename: Jing
authors.initials: J
authors.lastname: Shi
mesh_terms: D000596:Amino Acids / Q000138:chemical synthesis*; D060326:Chemistry Techniques, Synthetic; D006834:Hydrazines / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D015394:Molecular Structure; D010455:Peptides / Q000138:chemical synthesis*; D011486:Protein C / Q000737:chemistry*; D013438:Sulfhydryl Compounds / Q000138:chemical synthesis*
chemical_list: D000596:Amino Acids; D006834:Hydrazines; D010455:Peptides; D011486:Protein C; D013438:Sulfhydryl Compounds