title: Chikungunya virus glycoproteins pseudotype with lentiviral vectors and reveal a broad spectrum of cellular tropism.
abstract: BACKGROUND
  Outbreaks of the Chikungunya virus (CHIKV) infection has been documented in over 40 countries, resulting in clinical symptoms characterized by fever and joint pain. Diagnosing CHIKV in a clinical lab setting is often omitted because of the high lab safety requirement. An infection system that mimics CHIKV infection will permit clinical evaluation of the production of neutralizing antibody for both disease diagnostics and treatment.
  
  
  RESULTS
  We generated a CHIKV construct expressing CHIKV structural proteins. This construct permits the production of CHIKV pseudo-viral particles with a luciferase reporter. The pseudo-virus was able to infect a wide range of cell lines. The pseudovirus could be neutralized by the addition of neutralizing antibodies from patients.
  
  
  CONCLUSIONS
  Taken together, we have developed a powerful system that can be handled at biosafety level 2 laboratories for evaluation of existence of CHIKV neutralizing antibodies.
authors.affiliation: Department of Laboratory Medicine, Taizhou Municipal Hospital, Taizhou, China.
authors.forename: Dakang
authors.initials: D
authors.lastname: Hu
authors.affiliation: Department of Laboratory Medicine, Taizhou Municipal Hospital, Taizhou, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Institute of Infectious Diseases, Union Hospital, Tongji Medical Collage, Huazhong University of Science and Technology, Hubei Wuhan, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of Laboratory Medicine, Taizhou Municipal Hospital, Taizhou, China.
authors.forename: Shuangchun
authors.initials: S
authors.lastname: Liu
authors.affiliation: Department of Laboratory Medicine, Taizhou Municipal Hospital, Taizhou, China.
authors.forename: Lianhua
authors.initials: L
authors.lastname: Yu
authors.affiliation: Department of Laboratory Medicine, Taizhou Municipal Hospital, Taizhou, China.
authors.forename: Lingfen
authors.initials: L
authors.lastname: Sun
authors.affiliation: Department of Laboratory Medicine, Taizhou Municipal Hospital, Taizhou, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Qu
mesh_terms: D057134:Antibodies, Neutralizing / Q000008:administration & dosage / Q000276:immunology; D000914:Antibodies, Viral / Q000008:administration & dosage / Q000276:immunology; D065632:Chikungunya Fever / Q000175:diagnosis / Q000276:immunology / Q000628:therapy*; D002646:Chikungunya virus / Q000235:genetics* / Q000276:immunology / Q000472:pathogenicity; D005822:Genetic Vectors; D006023:Glycoproteins / Q000096:biosynthesis / Q000235:genetics* / Q000276:immunology; D006801:Humans; D016086:Lentivirus; D018523:Tropism; D015678:Viral Structural Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000276:immunology
chemical_list: D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D006023:Glycoproteins; D015678:Viral Structural Proteins