title: Geographic population structure of the sugarcane borer, Diatraea saccharalis (F.) (Lepidoptera: Crambidae), in the southern United States.
abstract: The sugarcane borer moth, Diatraea saccharalis, is widespread throughout the Western Hemisphere, and is considered an introduced species in the southern United States. Although this moth has a wide distribution and is a pest of many crop plants including sugarcane, corn, sorghum and rice, it is considered one species. The objective was to investigate whether more than one introduction of D. saccharalis had occurred in the southern United States and whether any cryptic species were present. We field collected D. saccharalis in Texas, Louisiana and Florida in the southern United States. Two molecular markers, AFLPs and mitochondrial COI, were used to examine genetic variation among these regional populations and to compare the sequences with those available in GenBank and BOLD. We found geographic population structure in the southern United States which suggests two introductions and the presence of a previously unknown cryptic species. Management of D. saccharalis would likely benefit from further investigation of population genetics throughout the range of this species.
authors.affiliation: SNRI, University of California Merced, Merced, California, United States of America.
authors.forename: Andrea L
authors.initials: AL
authors.lastname: Joyce
authors.affiliation: USDA-ARS Sugarcane Research Unit, Houma, Louisiana, United States of America.
authors.forename: William H
authors.initials: WH
authors.lastname: White
authors.affiliation: University of Florida, Everglades Research and Education Center, Belle Glade, Florida, United States of America.
authors.forename: Gregg S
authors.initials: GS
authors.lastname: Nuessly
authors.affiliation: USDA, Systematic Entomology, National Museum of Natural History, Washington, D. C., United States of America.
authors.forename: M Alma
authors.initials: MA
authors.lastname: Solis
authors.affiliation: USDA-ARS, Systematic Entomology Lab, Beltsville, Maryland, United States of America.
authors.forename: Sonja J
authors.initials: SJ
authors.lastname: Scheffer
authors.affiliation: USDA-ARS, Systematic Entomology Lab, Beltsville, Maryland, United States of America.
authors.forename: Matthew L
authors.initials: ML
authors.lastname: Lewis
authors.affiliation: Department of Entomology, Texas A&M University, College Station, Texas, United States of America.
authors.forename: Raul F
authors.initials: RF
authors.lastname: Medina
mesh_terms: D054458:Amplified Fragment Length Polymorphism Analysis; D000818:Animals; D001483:Base Sequence; D004272:DNA, Mitochondrial / Q000235:genetics*; D003576:Electron Transport Complex IV / Q000235:genetics*; D005260:Female; D005431:Florida; D019476:Insect Proteins / Q000235:genetics*; D058865:Introduced Species; D008145:Louisiana; D008297:Male; D008969:Molecular Sequence Data; D009036:Moths / Q000145:classification / Q000235:genetics*; D010802:Phylogeny; D058974:Phylogeography; D031786:Saccharum / Q000469:parasitology; D013781:Texas
chemical_list: D004272:DNA, Mitochondrial; D019476:Insect Proteins; D003576:Electron Transport Complex IV