title: Effect of the HBV whole-X gene on the expression of hepatocellular carcinoma associated proteins.
abstract: BACKGROUND
  The hepatitis B virus (HBV) pre-X gene resides upstream of the HBV X gene, and together they form the HBV whole-X gene. Although it has been evident that the HBV whole-X protein is involved in the development of hepatocellular carcinoma, its biological role and molecular mechanism remain largely unknown.
  
  
  METHODS
  In this study, we subcloned the HBV whole-X gene and constructed a HBV whole-X expressing vector. After transfection of the HBV whole-X gene into HL-7702 cells, the profile of the differential cellular protein composition in the cells was analyzed by using two-dimensional electrophoresis coupled to matrix-assisted laser desorption/ionization-time of flight mass spectrometry.
  
  
  RESULTS
  The results showed that 18 major proteins were differentially expressed in the cells transfected with or without the HBV whole-X gene. The expression of these genes was further confirmed by reverse transcription-polymerase chain reaction and Western blot analysis.
  
  
  CONCLUSIONS
  Our findings provide a new insight into the investigation of the pathological role that the HBV whole-X gene plays in the development of hepatocellular carcinoma and may lead to the design of novel strategies for the treatment of this disease.
authors.affiliation: Department of Infectious Diseases, First Affiliated Hospital of Medical College, Xi'an Jiaotong University, Xi'an 710061, Shaanxi Province, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Shaanxi Provincial Infectious Diseases Hospital, Xi'an 710061, Shaanxi Province, China; Xi'an Eighth Hospital Affiliated to Xi'an Jiaotong University Health Science Center, Xi'an 710061, Shaanxi Province, China.
authors.forename: Hongli
authors.initials: H
authors.lastname: Liu
authors.affiliation: Zhengzhou Sixth People's Hospital, Zhengzhou 450061, He'nan Province, China.
authors.forename: Meiling
authors.initials: M
authors.lastname: Cui
authors.affiliation: Department of Infectious Diseases, First Affiliated Hospital of Medical College, Xi'an Jiaotong University, Xi'an 710061, Shaanxi Province, China.
authors.forename: Jinfeng
authors.initials: J
authors.lastname: Liu
authors.affiliation: Department of Infectious Diseases, First Affiliated Hospital of Medical College, Xi'an Jiaotong University, Xi'an 710061, Shaanxi Province, China.
authors.forename: Ruitian
authors.initials: R
authors.lastname: Yi
authors.affiliation: Department of Infectious Diseases, First Affiliated Hospital of Medical College, Xi'an Jiaotong University, Xi'an 710061, Shaanxi Province, China.
authors.forename: Yinghua
authors.initials: Y
authors.lastname: Niu
authors.affiliation: Department of Infectious Diseases, First Affiliated Hospital of Medical College, Xi'an Jiaotong University, Xi'an 710061, Shaanxi Province, China.
authors.forename: Tianyan
authors.initials: T
authors.lastname: Chen
authors.affiliation: Department of Infectious Diseases, First Affiliated Hospital of Medical College, Xi'an Jiaotong University, Xi'an 710061, Shaanxi Province, China. Electronic address: zhaoyingren@mail.xjtu.edu.cn.
authors.forename: Yingren
authors.initials: Y
authors.lastname: Zhao
mesh_terms: D051017:Apoptosis Regulatory Proteins / Q000235:genetics; D006528:Carcinoma, Hepatocellular / Q000209:etiology / Q000821:virology*; D002460:Cell Line; D003001:Cloning, Molecular; D015180:Electrophoresis, Gel, Two-Dimensional; D020869:Gene Expression Profiling; D006515:Hepatitis B virus / Q000235:genetics; D006801:Humans; D008113:Liver Neoplasms / Q000209:etiology / Q000821:virology*; D009363:Neoplasm Proteins / Q000235:genetics / Q000378:metabolism*; D046988:Proteasome Endopeptidase Complex / Q000235:genetics; D040901:Proteomics / Q000379:methods; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D022021:Tacrolimus Binding Proteins / Q000235:genetics; D015534:Trans-Activators / Q000235:genetics*; D014162:Transfection / Q000379:methods; D054334:Viral Regulatory and Accessory Proteins
chemical_list: D051017:Apoptosis Regulatory Proteins; D009363:Neoplasm Proteins; C117283:PDCD5 protein, human; D015534:Trans-Activators; D054334:Viral Regulatory and Accessory Proteins; C057628:hepatitis B virus X protein; C074856:FKBP3 protein, human; C108898:PSMB4 protein, human; D046988:Proteasome Endopeptidase Complex; D022021:Tacrolimus Binding Proteins
keywords: HBV whole-X gene; Hepatitis B virus; hepatocellular carcinoma