title: Analysis of the heavy-chain modification and TSG-6 activity in pathological hyaluronan matrices.
abstract: During inflammation and developmental processes, heavy chains (HCs) from inter-α-inhibitor (IαI) are covalently transferred to hyaluronan (HA) via the enzyme tumor-necrosis-factor-stimulated-gene 6 (TSG-6) to form a HC-HA complex. In this manuscript, we describe a gel-based assay to detect HC-HA and TSG-6 activity in tissues.
authors.affiliation: Lerner Research Institute, Cleveland Clinic, 9500 Euclid Avenue, Cleveland, OH, 44195, USA, lauerm1@ccf.org.
authors.forename: Mark E
authors.initials: ME
authors.lastname: Lauer
authors.forename: Jacqueline
authors.initials: J
authors.lastname: Loftis
authors.forename: Carol
authors.initials: C
authors.lastname: de la Motte
authors.forename: Vincent C
authors.initials: VC
authors.lastname: Hascall
mesh_terms: D001249:Asthma / Q000473:pathology; D015153:Blotting, Western; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D003093:Colitis, Ulcerative / Q000473:pathology; D003106:Colon / Q000378:metabolism / Q000473:pathology*; D006801:Humans; D006820:Hyaluronic Acid / Q000378:metabolism*; D008168:Lung / Q000378:metabolism / Q000473:pathology*
chemical_list: D015815:Cell Adhesion Molecules; C072273:TNFAIP6 protein, human; D006820:Hyaluronic Acid