title: Isolation of giant plasma membrane vesicles for evaluation of plasma membrane structure and protein partitioning.
abstract: Although investigation into the structure of eukaryotic cell membranes has been an intense focus of cell biology for the past two decades, definitive insights have been limited by the lack of coherent methods for the isolation of specific organelle membranes and the identification of membrane subdomains. Here we describe a method for the isolation of mammalian cell plasma membranes as Giant Plasma Membrane Vesicles (GPMVs) and strategies for imaging membrane lateral structure and quantification of protein partitioning between coexisting domains by fluorescence microscopy.
authors.affiliation: Department of Integrative Biology and Pharmacology, University of Texas Health Science Center at Houston Medical School, 6431 Fannin Street, Houston, TX, 77030, USA, Kandice.R.Levental@uth.tmc.edu.
authors.forename: K R
authors.initials: KR
authors.lastname: Levental
authors.forename: I
authors.initials: I
authors.lastname: Levental
mesh_terms: D000818:Animals; D002458:Cell Fractionation; D002462:Cell Membrane / Q000737:chemistry*; D002478:Cells, Cultured; D004867:Equipment Design; D008322:Mammals; D008967:Molecular Biology / Q000295:instrumentation / Q000379:methods*; D015388:Organelles; D011506:Proteins / Q000302:isolation & purification
chemical_list: D011506:Proteins