title: Differential response of porcine immature monocyte-derived dendritic cells to virulent and inactivated transmissible gastroenteritis virus.
abstract: Exposure of piglets less than 2 weeks of age to virulent transmissible gastroenteritis virus (TGEV) gives rise to mortality as high as 100%, and adult pigs recovering from its infection often become TGEV carriers. These facts suggest an evasion of the immune system by virulent TGEV. In this study, we showed that a virulent TGEV SHXB strain could infect porcine immature monocyte-derived dendritic cells (Mo-DCs), and down-regulate cell surface markers (SLA-II-DR, CD1a and CD80/86). Moreover, SHXB-infected immature Mo-DCs showed low expression of IL-12 and IFN-γ, and also lost the ability to stimulate T cell proliferation. Finally, SHXB inhibited the activation of nuclear factor kappa B (NF-κB) in these cells. Instead, UV-inactivated SHXB (UV-SHXB) had the opposite effects in immature Mo-DCs. In conclusion, the virulent SHXB could severely impair immature Mo-DCs, which might be involved in the pathogenesis of virulent TGEV in vivo.
authors.affiliation: Key Lab of Animal Physiology and Biochemistry, Ministry of Agriculture, Nanjing Agricultural University, Wei gang 1, Jiangsu, China.
authors.forename: Shanshan
authors.initials: S
authors.lastname: Zhao
authors.affiliation: Key Lab of Animal Physiology and Biochemistry, Ministry of Agriculture, Nanjing Agricultural University, Wei gang 1, Jiangsu, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Gao
authors.affiliation: Key Lab of Animal Physiology and Biochemistry, Ministry of Agriculture, Nanjing Agricultural University, Wei gang 1, Jiangsu, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Lin
authors.affiliation: Key Lab of Animal Physiology and Biochemistry, Ministry of Agriculture, Nanjing Agricultural University, Wei gang 1, Jiangsu, China.
authors.forename: Mengfei
authors.initials: M
authors.lastname: Yan
authors.affiliation: Key Lab of Animal Physiology and Biochemistry, Ministry of Agriculture, Nanjing Agricultural University, Wei gang 1, Jiangsu, China.
authors.forename: Qinghua
authors.initials: Q
authors.lastname: Yu
authors.affiliation: Key Lab of Animal Physiology and Biochemistry, Ministry of Agriculture, Nanjing Agricultural University, Wei gang 1, Jiangsu, China. Electronic address: zxbyq@njau.edu.cn.
authors.forename: Qian
authors.initials: Q
authors.lastname: Yang
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D015153:Blotting, Western; D003713:Dendritic Cells / Q000276:immunology* / Q000821:virology; D004797:Enzyme-Linked Immunosorbent Assay; D005434:Flow Cytometry; D005761:Gastroenteritis, Transmissible, of Swine / Q000276:immunology*; D015967:Gene Expression Regulation, Viral / Q000502:physiology*; D057131:Immune Evasion / Q000276:immunology*; D018664:Interleukin-12 / Q000378:metabolism; D008213:Lymphocyte Activation / Q000276:immunology; D009000:Monocytes / Q000166:cytology* / Q000276:immunology; D016328:NF-kappa B / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction / Q000662:veterinary; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D013552:Swine; D013601:T-Lymphocytes / Q000276:immunology; D005760:Transmissible gastroenteritis virus / Q000276:immunology* / Q000472:pathogenicity*; D014774:Virulence
chemical_list: D016328:NF-kappa B; D018664:Interleukin-12
keywords: NF-κB; Porcine monocyte-derived dendritic cells (Mo-DCs); T cell proliferation; Virulent transmissible gastroenteritis virus