title: Paper-based molecular diagnostic forChlamydia trachomatis.
abstract: Herein we show the development of a minimally instrumented paper-based molecular diagnostic for point of care detection of sexually transmitted infections caused byChlamydia trachomatis. This new diagnostic platform incorporates cell lysis, isothermal nucleic acid amplification, and lateral flow visual detection using only a pressure source and heat block, eliminating the need for expensive laboratory equipment. This paper-based test can be performed in less than one hour and has a clinically relevant limit of detection that is 100x more sensitive than current rapid immunoassays used for chlamydia diagnosis.
authors.affiliation: Biomedical Engineering, Boston University, Boston, MA, 02215.
authors.forename: Jacqueline C
authors.initials: JC
authors.lastname: Linnes
authors.affiliation: Biomedical Engineering, Boston University, Boston, MA, 02215.
authors.forename: Andy
authors.initials: A
authors.lastname: Fan
authors.affiliation: Biomedical Engineering, Boston University, Boston, MA, 02215.
authors.forename: Natalia M
authors.initials: NM
authors.lastname: Rodriguez
authors.affiliation: BioHelix Corporation, Beverly, MA, 01915.
authors.forename: Bertrand
authors.initials: B
authors.lastname: Lemieux
authors.affiliation: BioHelix Corporation, Beverly, MA, 01915.
authors.forename: Huimin
authors.initials: H
authors.lastname: Kong
authors.affiliation: Biomedical Engineering, Boston University, Boston, MA, 02215.
authors.forename: Catherine M
authors.initials: CM
authors.lastname: Klapperich