title: Cathepsin L is involved in proliferation and invasion of ovarian cancer cells.
abstract: Cathepsin L (CTSL) is a lysosomal cysteine protease that has been found to be overexpressed in ovarian cancer (OC). The aim of the present study was to investigate the possible involvement of CTSL in the development of OC. In this study, RNA interference with a CTSL small hairpin RNA (CTSL-shRNA), and a plasmid carrying CTSL were used to identify the effects of this enzyme on the regulation of the malignant behavior of OC cells. OV-90 and SKOV3 human ovarian cancer cell lines were selected as cell models in vitro and in vivo. The results showed that downregulation of CTSL significantly inhibits the proliferative and invasive capability of SKOV3 cells, and that upregulation of CTSL in OV-90 cells leads to opposite effects. Compared with parental OC cells, cells in which CTSL was silenced exhibited a reduced capacity to develop into tumors in nude mice, while the growth of tumor xenografts derived from these cells was markedly constrained. In conclusion, the results suggested that CTSL contributes to the proliferation and metastasis of OC, and that CTSL may be a novel molecular target for OC treatment.
authors.affiliation: Department of Oncology, The 458th Hospital of PLA, Guangzhou, Guangdong 510602, P.R. China.
authors.forename: Luosheng
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department of Oncology, The 458th Hospital of PLA, Guangzhou, Guangdong 510602, P.R. China.
authors.forename: Lixia
authors.initials: L
authors.lastname: Wei
authors.affiliation: Department of Oncology, Sun Yat‑sen University Cancer Center, Guangzhou, Guangdong 510000, P.R. China.
authors.forename: Guanzhu
authors.initials: G
authors.lastname: Shen
authors.affiliation: Department of Oncology, The 421th Hospital of PLA, Guangzhou, Guangdong 510000, P.R. China.
authors.forename: Benfu
authors.initials: B
authors.lastname: He
authors.affiliation: Department of Oncology, The 458th Hospital of PLA, Guangzhou, Guangdong 510602, P.R. China.
authors.forename: Wei
authors.initials: W
authors.lastname: Gong
authors.affiliation: Department of Oncology, The 458th Hospital of PLA, Guangzhou, Guangdong 510602, P.R. China.
authors.forename: Na
authors.initials: N
authors.lastname: Min
authors.affiliation: Department of Oncology, The 458th Hospital of PLA, Guangzhou, Guangdong 510602, P.R. China.
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department of Oncology, The 458th Hospital of PLA, Guangzhou, Guangdong 510602, P.R. China.
authors.forename: Yuxin
authors.initials: Y
authors.lastname: Duan
authors.affiliation: Department of Oncology, The 458th Hospital of PLA, Guangzhou, Guangdong 510602, P.R. China.
authors.forename: Jingran
authors.initials: J
authors.lastname: Xie
authors.affiliation: Department of Oncology, The 458th Hospital of PLA, Guangzhou, Guangdong 510602, P.R. China.
authors.forename: Huiyan
authors.initials: H
authors.lastname: Luo
authors.affiliation: Department of Oncology, The 458th Hospital of PLA, Guangzhou, Guangdong 510602, P.R. China.
authors.forename: Xingwang
authors.initials: X
authors.lastname: Gao
mesh_terms: D000818:Animals; D056668:Cathepsin L / Q000235:genetics*; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation; D004195:Disease Models, Animal; D005260:Female; D015870:Gene Expression; D064593:Heterografts; D006801:Humans; D008297:Male; D020928:Mitogen-Activated Protein Kinases; D010051:Ovarian Neoplasms / Q000235:genetics* / Q000473:pathology; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics; D047368:Tumor Burden; D014410:Tumor Stem Cell Assay
chemical_list: D034741:RNA, Small Interfering; D020928:Mitogen-Activated Protein Kinases; D056668:Cathepsin L