title: Measuring cytoskeleton and cellular membrane mechanical properties by atomic force microscopy.
abstract: Atomic force microscope is an invaluable device to explore living specimens at a nanometric scale. It permits to image the topography of the sample in 3D, to measure its mechanical properties and to detect the presence of specific molecules bound on its surface. Here we describe the procedure to gather such a data set on living macrophages.
authors.affiliation: Laboratoire de Physique de la Matière Vivante, IPSB, SB, EPFL, Lausanne, Switzerland.
authors.forename: Charles
authors.initials: C
authors.lastname: Roduit
authors.forename: Giovanni
authors.initials: G
authors.lastname: Longo
authors.forename: Giovanni
authors.initials: G
authors.lastname: Dietler
authors.forename: Sandor
authors.initials: S
authors.lastname: Kasas
mesh_terms: D018929:Cell Culture Techniques; D002462:Cell Membrane / Q000737:chemistry*; D003599:Cytoskeleton / Q000737:chemistry*; D006801:Humans; D021621:Imaging, Three-Dimensional / Q000379:methods; D008264:Macrophages / Q000166:cytology*; D018625:Microscopy, Atomic Force / Q000295:instrumentation / Q000379:methods*