title: Effects of monocrotophos pesticide on steroidogenesis and transcription of steroidogenic enzymes in rainbow trout RTG-2 cells involving the protein kinase A signal pathway.
abstract: Monocrotophos (MCP) pesticide, listed as a UNEP Prior Informed Consent chemical, has been proved to exert toxic effects on the reproductive system of teleost fishes by changing the balance of sex steroid hormones. To investigate the effects of MCP on steroidogenesis in vitro, the rainbow trout (Oncorhynchus mykiss) gonadal cell line RTG-2 was exposed to different MCP concentrations for 48 h. The levels of 17 β-estradiol (E(2)) and testosterone in the medium were measured by radioimmunoassay and the expression of steroidogenic acute regulatory protein and cytochrome P450 enzymes CYP11A1, CYP17, and CYP19A was detected by quantitative real-time PCR. The results showed that 1.0 and 10.0 μg/L MCP pesticide induced E(2) levels and promoted steroidogenic enzyme expression. The possible mechanisms of MCP steroidogenic activity were investigated using inhibitors of protein kinase A (PKA) and protein kinase C. The PKA inhibitor H-89 abrogated the 10.0 μg/L MCP-induced transcriptional up-regulation of steroidogenic enzymes, suggesting an involvement of PKA-dependent mechanism in the disruption of steroidogenesis by the MCP pesticide in rainbow trout RTG-2 cells.
authors.affiliation: Marine Life Science College, Ocean University of China, Qingdao 266003, China.
authors.forename: Zhenyu
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Marine Life Science College, Ocean University of China, Qingdao 266003, China.
authors.forename: Xiaona
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Marine Life Science College, Ocean University of China, Qingdao 266003, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Tian
authors.affiliation: Marine Life Science College, Ocean University of China, Qingdao 266003, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.affiliation: Marine Life Science College, Ocean University of China, Qingdao 266003, China. Electronic address: rusg@ouc.edu.cn.
authors.forename: Shaoguo
authors.initials: S
authors.lastname: Ru
mesh_terms: D000818:Animals; D001141:Aromatase / Q000096:biosynthesis / Q000187:drug effects; D002460:Cell Line; D002786:Cholesterol Side-Chain Cleavage Enzyme / Q000096:biosynthesis / Q000187:drug effects; D017868:Cyclic AMP-Dependent Protein Kinases / Q000187:drug effects*; D052244:Endocrine Disruptors / Q000633:toxicity; D004958:Estradiol / Q000096:biosynthesis*; D006066:Gonads / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D007306:Insecticides / Q000633:toxicity*; D008999:Monocrotophos / Q000633:toxicity*; D017686:Oncorhynchus mykiss / Q000378:metabolism / Q000502:physiology; D060888:Real-Time Polymerase Chain Reaction; D015398:Signal Transduction / Q000187:drug effects*; D013254:Steroid 17-alpha-Hydroxylase / Q000096:biosynthesis / Q000187:drug effects; D013739:Testosterone / Q000096:biosynthesis*
chemical_list: D052244:Endocrine Disruptors; D007306:Insecticides; D013739:Testosterone; D004958:Estradiol; D008999:Monocrotophos; D001141:Aromatase; D013254:Steroid 17-alpha-Hydroxylase; D002786:Cholesterol Side-Chain Cleavage Enzyme; D017868:Cyclic AMP-Dependent Protein Kinases
keywords: Monocrotophos; Protein kinase A; RTG-2 cell line; Steroidogenesis; Steroidogenic enzymes