title: The Pseudomonas aeruginosa Mannose Sensitive Hamemagglutination Strain (PA-MSHA) Induces a Th1-Polarizing Phenotype by Promoting Human Dendritic Cells Maturation.
abstract: Pseudomonas aeruginosa mannose sensitive hamemagglutination strain (PA-MSHA) is a kind of peritrichous P. aeruginosa strain with MSHA fimbriae and has been shown to activate kinds of immunocytes. Dendritic cells (DCs) are specialized antigen-presenting cells required for the stimulating and priming CD4(+) T cells toward the T helper cell type 1 (Th1), Th2 and other different phenotypes. PA-MSHA effecting on Th1 remains an important missing link. Here we demonstrated that PA-MSHA augmented monocytes derived-dendritic cells (Mo-DCs) expression of HLA-DR, co-stimulatory and adhesion molecules, and induced Th1-promoting interleukin-12 and tumor necrosis factor α secretion, in addition, PA-MSHA treated Mo-DCs displayed lesser endocytic capacity. Furthermore, in mixed lymphocyte reactions, allostimulatory capacity of Mo-DCs was enhanced by PA-MSHA, CD4(+) T cells stimulated by PA-MSHA -activated Mo-DCs showed a Th1-polarized cytokine production, increasing secretion of IFN-γ and decreasing secretion of IL-10 and IL-4. Our findings identified PA-MSHA as an important exogenous factor that induced DCs maturation toward a Th1-promoting phenotype.
authors.affiliation: Department of Stomatology, Guangzhou Women and Children's Medical Center, Guangzhou, China.
authors.forename: Yunyan
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Stomatology, Guangzhou Women and Children's Medical Center, Guangzhou, China.
authors.forename: Hongtao
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of Laboratory Medicine, Guangdong Provincial Hospital of Chinese Medicine, Guangzhou, China.
authors.forename: Youqiang
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Stomatology, Guangzhou Women and Children's Medical Center, Guangzhou, China.
authors.forename: Ke
authors.initials: K
authors.lastname: Chen
authors.affiliation: Department of Stomatology, Guangzhou Women and Children's Medical Center, Guangzhou, China.
authors.forename: Jinmei
authors.initials: J
authors.lastname: Ye
authors.affiliation: Department of Oral and Maxillofacial Surgery, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Liao
authors.affiliation: Department of Stomatology, Guangzhou Women and Children's Medical Center, Guangzhou, China.
authors.forename: Yiyang
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Department of Oral and Maxillofacial Surgery, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Ran
keywords: Dendritic cells; Differentiation of Th cell; Immunomodulatory capacity; PA-MSHA strain