title: A biorefinery approach based on fractionation with a cheap industrial by-product for getting value from an invasive woody species.
abstract: Acacia dealbata wood (an invasive species) was subjected to fractionation with glycerol (a cheap industrial by-product), and the resulting solid phase was used as a substrate for enzymatic hydrolysis. Glycerol fractionation allowed an extensive delignification while preserving cellulose in solid phase. The solids from the fractionation stage showed high susceptibility to enzymatic hydrolysis. Solids obtained under selected fractionation conditions (glycerol content of media, 80 wt%; duration, 1h; liquid to solid ratio, 6 g/g; alkaline and neutral washing stages) were subjected to enzymatic saccharification to achieve glucose concentrations up to 85.40 g/L, with almost complete cellulose conversion into glucose. The results confirmed the potential of glycerol as a fractionation agent for biorefineries.
authors.affiliation: Department of Chemical Engineering, Faculty of Science, University of Vigo (Campus Ourense), As Lagoas, 32004 Ourense, Spain; CITI-Tecnopole, San Ciprian de Viñas, 32901 Ourense, Spain.
authors.forename: Elena
authors.initials: E
authors.lastname: Domínguez
authors.affiliation: CEB-Centre of Biological Engineering, University of Minho, Campus Gualtar, 4710-057 Braga, Portugal.
authors.forename: Aloia
authors.initials: A
authors.lastname: Romaní
authors.affiliation: Department of Chemical Engineering, Faculty of Science, University of Vigo (Campus Ourense), As Lagoas, 32004 Ourense, Spain; CITI-Tecnopole, San Ciprian de Viñas, 32901 Ourense, Spain.
authors.forename: José Luis
authors.initials: JL
authors.lastname: Alonso
authors.affiliation: Department of Chemical Engineering, Faculty of Science, University of Vigo (Campus Ourense), As Lagoas, 32004 Ourense, Spain; CITI-Tecnopole, San Ciprian de Viñas, 32901 Ourense, Spain.
authors.forename: Juan Carlos
authors.initials: JC
authors.lastname: Parajó
authors.affiliation: Department of Chemical Engineering, Faculty of Science, University of Vigo (Campus Ourense), As Lagoas, 32004 Ourense, Spain; CITI-Tecnopole, San Ciprian de Viñas, 32901 Ourense, Spain. Electronic address: reme@uvigo.es.
authors.forename: Remedios
authors.initials: R
authors.lastname: Yáñez
mesh_terms: D000045:Acacia / Q000737:chemistry*; D002480:Cellulase / Q000378:metabolism; D002482:Cellulose / Q000378:metabolism; D006868:Hydrolysis; D058865:Introduced Species; D014934:Wood
chemical_list: D002482:Cellulose; D002480:Cellulase
keywords: Acacia dealbata; Enzymatic hydrolysis; Glycerol; Pretreatment