title: Apolipoprotein C3 (-455T>C) polymorphism confers susceptibility to nonalcoholic fatty liver disease in the Southern Han Chinese population.
abstract: OBJECTIVE
  To investigate the relationship between Apolipoprotein C3 (APOC3) (-455T>C) polymorphism and nonalcoholic fatty liver disease (NAFLD) in the Southern Chinese Han population.
  
  
  METHODS
  In this prospective case-control study, we recruited 300 NAFLD patients and 300 healthy controls to a cohort representing Southern Chinese Han population at The First Affiliated Hospital, Sun Yat-sen University, from January to December 2012. Polymerase chain reaction-restriction fragment length polymorphism and DNA sequencing were used to genotype the APOC3 (-455T>C) variants.
  
  
  RESULTS
  After adjusting for age, gender, and body-mass index, TC and CC genotypes were found to increase the susceptibility to NAFLD compared to the TT genotype, with adjusted odds ratios (ORs) of 1.77 (95%CI: 1.16-2.72) and 2.80 (95%CI: 1.64-4.79), respectively. Further stratification analysis indicated that carriers of the CC genotype was more susceptible to insulin resistance (IR) than those of the TT genotype, with an OR of 3.24 (95%CI: 1.52-6.92). The CC genotype also was associated with a significantly higher risk of hypertension, hypertriglyceridemia, and low levels of high-density lipoprotein cholesterol (HDL) (P < 0.05). No association was found between the APOC3 (-455T>C) polymorphism and obesity, impaired glucose tolerance, hyperuricemia, hypercholesterolemia, or high levels of low-density lipoprotein cholesterol (LDL) (P > 0.05).
  
  
  CONCLUSIONS
  APOC3 (-455T>C) genetic variation is involved in the susceptibility to developing NAFLD, IR, hypertension, hypertriglyceridemia, and low HDL in the Southern Chinese Han population.
authors.affiliation: Min-Rui Li, Sheng-Hong Zhang, Kang Chao, Xian-Hua Liao, Jia-Yan Yao, Min-Hu Chen, Bi-Hui Zhong, Division of Gastroenterology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, Guangdong Province, China.
authors.forename: Min-Rui
authors.initials: MR
authors.lastname: Li
authors.affiliation: Min-Rui Li, Sheng-Hong Zhang, Kang Chao, Xian-Hua Liao, Jia-Yan Yao, Min-Hu Chen, Bi-Hui Zhong, Division of Gastroenterology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, Guangdong Province, China.
authors.forename: Sheng-Hong
authors.initials: SH
authors.lastname: Zhang
authors.affiliation: Min-Rui Li, Sheng-Hong Zhang, Kang Chao, Xian-Hua Liao, Jia-Yan Yao, Min-Hu Chen, Bi-Hui Zhong, Division of Gastroenterology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, Guangdong Province, China.
authors.forename: Kang
authors.initials: K
authors.lastname: Chao
authors.affiliation: Min-Rui Li, Sheng-Hong Zhang, Kang Chao, Xian-Hua Liao, Jia-Yan Yao, Min-Hu Chen, Bi-Hui Zhong, Division of Gastroenterology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, Guangdong Province, China.
authors.forename: Xian-Hua
authors.initials: XH
authors.lastname: Liao
authors.affiliation: Min-Rui Li, Sheng-Hong Zhang, Kang Chao, Xian-Hua Liao, Jia-Yan Yao, Min-Hu Chen, Bi-Hui Zhong, Division of Gastroenterology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, Guangdong Province, China.
authors.forename: Jia-Yan
authors.initials: JY
authors.lastname: Yao
authors.affiliation: Min-Rui Li, Sheng-Hong Zhang, Kang Chao, Xian-Hua Liao, Jia-Yan Yao, Min-Hu Chen, Bi-Hui Zhong, Division of Gastroenterology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, Guangdong Province, China.
authors.forename: Min-Hu
authors.initials: MH
authors.lastname: Chen
authors.affiliation: Min-Rui Li, Sheng-Hong Zhang, Kang Chao, Xian-Hua Liao, Jia-Yan Yao, Min-Hu Chen, Bi-Hui Zhong, Division of Gastroenterology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, Guangdong Province, China.
authors.forename: Bi-Hui
authors.initials: BH
authors.lastname: Zhong
mesh_terms: D000328:Adult; D053305:Apolipoprotein C-III / Q000235:genetics*; D044466:Asian People / Q000235:genetics; D016022:Case-Control Studies; D016009:Chi-Square Distribution; D002681:China / Q000453:epidemiology; D015897:Comorbidity; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D006785:Hospitals, University; D006801:Humans; D016015:Logistic Models; D008297:Male; D008875:Middle Aged; D015999:Multivariate Analysis; D065626:Non-alcoholic Fatty Liver Disease / Q000175:diagnosis / Q000208:ethnology / Q000235:genetics*; D016017:Odds Ratio; D010641:Phenotype; D011110:Polymorphism, Genetic; D011446:Prospective Studies; D012307:Risk Factors
chemical_list: D053305:Apolipoprotein C-III
keywords: Apolipoprotein C3; Insulin resistance; Metabolic disorder; Nonalcoholic fatty liver disease; Polymorphism