title: Antioxidant and protective effect of inulin and catechin grafted inulin against CCl4-induced liver injury.
abstract: In this study, the antioxidant activity and hepatoprotective effect of inulin and catechin grafted inulin (catechin-g-inulin) against carbon tetrachloride (CCl4)-induced acute liver injury were investigated. Results showed that both inulin and catechin-g-inulin had moderate scavenging activity on superoxide radical, hydroxyl radical and H2O2, as well as lipid peroxidation inhibition effect. The antioxidant activity decreased in the order of Vc > catechin >catechin-g-inulin > inulin. Administration of inulin and catechin-g-inulin could significantly reduce the elevated levels of serum aspartate transaminase, alanine transaminase and alkaline phosphatase as compared to CCl4 treatment group. Moreover, inulin and catechin-g-inulin significantly increased the levels of hepatic superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase, glutathione and total antioxidant capacity, whereas markedly decreased the malondialdehyde level when compared with CCl4 treatment group. Notably, catechin-g-inulin showed higher hepatoprotective effect than inulin. In addition, the hepatoprotective effect of catechin-g-inulin was comparable to positive standard of silymarin. Our results suggested that catechin-g-inulin had potent antioxidant activity and potential protective effect against CCl4-induced acute liver injury.
authors.affiliation: College of Food Science and Engineering, Yangzhou University, Yangzhou 225127, Jiangsu, China. Electronic address: junliu@yzu.edu.cn.
authors.forename: Jun
authors.initials: J
authors.lastname: Liu
authors.affiliation: College of Food Science and Engineering, Yangzhou University, Yangzhou 225127, Jiangsu, China.
authors.forename: Jian-feng
authors.initials: JF
authors.lastname: Lu
authors.affiliation: College of Food Science and Engineering, Yangzhou University, Yangzhou 225127, Jiangsu, China.
authors.forename: Xiao-yuan
authors.initials: XY
authors.lastname: Wen
authors.affiliation: College of Food Science and Engineering, Yangzhou University, Yangzhou 225127, Jiangsu, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Kan
authors.affiliation: College of Food Science and Engineering, Yangzhou University, Yangzhou 225127, Jiangsu, China.
authors.forename: Chang-hai
authors.initials: CH
authors.lastname: Jin
mesh_terms: D000410:Alanine Transaminase / Q000097:blood; D000469:Alkaline Phosphatase / Q000097:blood; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001219:Aspartate Aminotransferases / Q000097:blood; D001835:Body Weight / Q000187:drug effects; D002251:Carbon Tetrachloride; D002392:Catechin / Q000494:pharmacology*; D005978:Glutathione / Q000378:metabolism; D007444:Inulin / Q000494:pharmacology*; D008099:Liver / Q000187:drug effects* / Q000201:enzymology / Q000473:pathology*; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D008813:Mice, Inbred ICR; D009929:Organ Size / Q000187:drug effects; D020011:Protective Agents / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D000975:Antioxidants; D020011:Protective Agents; D017382:Reactive Oxygen Species; D008315:Malondialdehyde; D002392:Catechin; D007444:Inulin; D002251:Carbon Tetrachloride; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D000469:Alkaline Phosphatase; D005978:Glutathione
keywords: Antioxidant activity; Catechin; Grafted; Hepatoprotective effect; Inulin