title: MicroRNA-125b inhibits lens epithelial cell apoptosis by targeting p53 in  age-related cataract.
abstract: MicroRNA-125b (miR-125b) has been implicated in a variety of diseases as either repressors or promoters, and plays crucial roles in many cellular processes such as cell differentiation, proliferation and apoptosis. Age-related cataract has become one of the most serious problems facing the aging population in the world. The purpose of this study was to investigate the role of miR-125b in the development of age-related cataract. We demonstrated that miR-125b was downregulated in both age-related cataract tissue and lens epithelial cell apoptosis induced by UV irradiation. We also identified the impact of miR-125b on apoptosis in a lens epithelial cell line. In vitro, miR-125b regulates human lens epithelial cell apoptosis at least in part by directly targeting p53. In addition,an inverse relationship between miR-125b and p53 expression was seen in age-related cataract tissue. In conclusion,this study suggests that miR-125b might be closely involved in the pathogenesis of cataract, and has the potential to be a diagnostic biomarker or even a therapeutic modality for cataract.
authors.forename: Yu
authors.initials: Y
authors.lastname: Qin
authors.forename: Jiangyue
authors.initials: J
authors.lastname: Zhao
authors.forename: Xiaojie
authors.initials: X
authors.lastname: Min
authors.forename: Mingwu
authors.initials: M
authors.lastname: Wang
authors.forename: Wenting
authors.initials: W
authors.lastname: Luo
authors.forename: Di
authors.initials: D
authors.lastname: Wu
authors.forename: Qichang
authors.initials: Q
authors.lastname: Yan
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.forename: Xinwei
authors.initials: X
authors.lastname: Wu
authors.forename: Jinsong
authors.initials: J
authors.lastname: Zhang
mesh_terms: D000367:Age Factors; D017209:Apoptosis / Q000235:genetics* / Q000528:radiation effects; D015153:Blotting, Western; D002386:Cataract / Q000235:genetics* / Q000473:pathology; D002460:Cell Line; D002478:Cells, Cultured; D015536:Down-Regulation; D004847:Epithelial Cells / Q000378:metabolism*; D005434:Flow Cytometry; D015870:Gene Expression; D006801:Humans; D020287:In Situ Nick-End Labeling; D007908:Lens, Crystalline / Q000378:metabolism* / Q000473:pathology; D035683:MicroRNAs / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016159:Tumor Suppressor Protein p53 / Q000235:genetics*; D014466:Ultraviolet Rays
chemical_list: C531888:MIRN125 microRNA, human; D035683:MicroRNAs; D016159:Tumor Suppressor Protein p53