title: [Effects of small interfering RNA silencing MACC-1 expression on cell proliferation, cell cycle and invasion ability of cervical cancer SiHa cells].
abstract: OBJECTIVE
  To investigate the expression of metastasis-associated in colon cancer-1 (MACC-1) mediated by siRNA, and to study the effects of its downregulation on cell proliferation, cell cycle and invasion ability of cervical cancer SiHa cells.
  
  
  METHODS
  MACC-1 siRNA and control siRNA were transfected into cervical cancer SiHa cells, and the expression of MACC-1 protein after transfection with MACC-1 siRNA was detected by Western blotting. The changes of cell proliferation, cell cycle and invasion ability of the SiHa cells were determined by CCK-8 kit, flow cytometry and Boyden chamber assay. The expressions of cell cycle- and invasion-related proteins were analyzed by Western blotting.
  
  
  RESULTS
  Compared with the untreated group (0.317 ± 0.023) and control siRNA group (0.309 ± 0.021), the expression of MACC-1 protein was downregulated in the MACC1 siRNA group (0.041 ± 0.006) (P < 0.05), and its downregulation significantly suppressed the cell proliferation, altered the cell cycle distribution and reduced the cell invasion ability of the SiHa cells (P < 0.05). Compared with the untreated group (0.217 ± 0.025 and 0.215 ± 0.024) and the control siRNA group (0.222 ± 0.023 and 0.207 ± 0.027), the expression of cyclin D1 and Cdk2 proteins were significantly decreased in the MACC1 siRNA group (0.076 ± 0.010 and 0.039 ± 0.007) (P < 0.05). Compared with the untreated group (0.099 ± 0.007) and control siRNA group (0.105 ± 0.012), the expression of p21 protein was significantly increased in the MACC1 siRNA group (0.676 ± 0.044) (P < 0.05). The downregulation of MACC-1 expression also evoked a decrease of expressions of MMP-2 and MMP-9 proteins and an increase of E-cadherin protein expression (P < 0.05).
  
  
  CONCLUSIONS
  MACC-1 downregulation-mediated inhibition of proliferation and decreased invasion ability of tumor cells may be closely associated with the alterations of expressions of cell cycle- and invasion-related proteins.
authors.affiliation: Department of Obstetrics and Gynecology, the First Affiliated Hospital, Xinxiang Medical University, Xinxiang 453100, China.
authors.forename: Fangfang
authors.initials: F
authors.lastname: Hua
authors.forename: Yonghua
authors.initials: Y
authors.lastname: Xia
authors.forename: Huiling
authors.initials: H
authors.lastname: Wang
authors.forename: Ruixiang
authors.initials: R
authors.lastname: Chen
authors.forename: Yanfang
authors.initials: Y
authors.lastname: Ren
authors.forename: Jun
authors.initials: J
authors.lastname: Yang
authors.affiliation: Email: wufengliang11@sina.com.
authors.forename: Wufeng
authors.initials: W
authors.lastname: Liang
mesh_terms: D015820:Cadherins / Q000378:metabolism; D002453:Cell Cycle; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation / Q000187:drug effects; D019938:Cyclin D1 / Q000378:metabolism; D051357:Cyclin-Dependent Kinase 2 / Q000378:metabolism; D015536:Down-Regulation; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D020868:Gene Silencing; D006801:Humans; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D034741:RNA, Small Interfering; D015534:Trans-Activators; D014157:Transcription Factors / Q000378:metabolism*; D014162:Transfection; D002583:Uterine Cervical Neoplasms / Q000378:metabolism*
chemical_list: D015820:Cadherins; C544162:MACC1 protein, human; D034741:RNA, Small Interfering; D015534:Trans-Activators; D014157:Transcription Factors; D019938:Cyclin D1; D051357:Cyclin-Dependent Kinase 2; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9