title: Sox2 is involved in paclitaxel resistance of the prostate cancer cell line PC-3 via the PI3K/Akt pathway.
abstract: Prostate cancer is the most commonly diagnosed type of cancer and the second leading cause of cancer‑associated mortality in males. The efficacy of prostate cancer chemotherapy is frequently impaired by drug resistance; however, the underlying mechanisms of this resistance remain elusive. Sex determining region Y-box 2 (Sox2) is of vital importance in the regulation of stem cell proliferation and carcinogenesis. In the present study, using MTT, clone formation, cell cycle and apoptosis assays, over-expression of Sox2 was demonstrated to enhance the paclitaxel (Pac) resistance of the PC-3 prostate cancer cell line, promoting cell proliferation and exhibiting an anti‑apoptotic effect. Western blot analysis revealed that the phosphoinositide 3-kinase/Akt signaling pathway was activated in cells overexpressing Sox2, and by targeting cyclin E and survivin, Sox2 promoted G1/S phase transition and prevented apoptosis under Pac treatment. The present study provided an understanding of Pac resistance in prostate cancer and may indicate novel therapeutic methods for chemoresistant prostate cancer.
authors.affiliation: Department of Biochemistry and Molecular Biology, Harbin Medical University, Harbin, Heilongjiang 150081, P.R. China.
authors.forename: Dong
authors.initials: D
authors.lastname: Li
authors.affiliation: Department of Biochemistry and Molecular Biology, Harbin Medical University, Harbin, Heilongjiang 150081, P.R. China.
authors.forename: Li-Nan
authors.initials: LN
authors.lastname: Zhao
authors.affiliation: Department of Ultrasonography, Harbin Medical University Cancer Hospital, Harbin, Heilongjiang 150081, P.R. China.
authors.forename: Xiu-Lan
authors.initials: XL
authors.lastname: Zheng
authors.affiliation: Department of Biochemistry and Molecular Biology, Harbin Medical University, Harbin, Heilongjiang 150081, P.R. China.
authors.forename: Ping
authors.initials: P
authors.lastname: Lin
authors.affiliation: Department of Biochemistry and Molecular Biology, Harbin Medical University, Harbin, Heilongjiang 150081, P.R. China.
authors.forename: Feng
authors.initials: F
authors.lastname: Lin
authors.affiliation: Department of Biochemistry and Molecular Biology, Harbin Medical University, Harbin, Heilongjiang 150081, P.R. China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Biochemistry and Molecular Biology, Harbin Medical University, Harbin, Heilongjiang 150081, P.R. China.
authors.forename: Hai-Feng
authors.initials: HF
authors.lastname: Zou
authors.affiliation: Department of Biochemistry and Molecular Biology, Harbin Medical University, Harbin, Heilongjiang 150081, P.R. China.
authors.forename: Rong-Jun
authors.initials: RJ
authors.lastname: Cui
authors.affiliation: Department of Urology, the Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150081, P.R. China.
authors.forename: Hui
authors.initials: H
authors.lastname: Chen
authors.affiliation: Department of Biochemistry and Molecular Biology, Harbin Medical University, Harbin, Heilongjiang 150081, P.R. China.
authors.forename: Xiao-Guang
authors.initials: XG
authors.lastname: Yu
mesh_terms: D017209:Apoptosis / Q000187:drug effects / Q000235:genetics; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects / Q000235:genetics; D019927:Cyclin E / Q000235:genetics; D019008:Drug Resistance, Neoplasm / Q000235:genetics*; D016193:G1 Phase / Q000187:drug effects / Q000235:genetics; D006801:Humans; D051034:Inhibitor of Apoptosis Proteins / Q000235:genetics; D008297:Male; D017239:Paclitaxel / Q000494:pharmacology*; D019869:Phosphatidylinositol 3-Kinases / Q000235:genetics*; D011471:Prostatic Neoplasms / Q000188:drug therapy* / Q000235:genetics*; D051057:Proto-Oncogene Proteins c-akt / Q000235:genetics*; D016196:S Phase / Q000187:drug effects / Q000235:genetics; D055748:SOXB1 Transcription Factors / Q000235:genetics*; D015398:Signal Transduction / Q000187:drug effects / Q000235:genetics; D000077022:Survivin
chemical_list: C107489:BIRC5 protein, human; D019927:Cyclin E; D051034:Inhibitor of Apoptosis Proteins; C527782:SOX2 protein, human; D055748:SOXB1 Transcription Factors; D000077022:Survivin; D019869:Phosphatidylinositol 3-Kinases; D051057:Proto-Oncogene Proteins c-akt; D017239:Paclitaxel