title: Dynamic evolution of the GnRH receptor gene family in vertebrates.
abstract: BACKGROUND
  Elucidating the mechanisms underlying coevolution of ligands and receptors is an important challenge in molecular evolutionary biology. Peptide hormones and their receptors are excellent models for such efforts, given the relative ease of examining evolutionary changes in genes encoding for both molecules. Most vertebrates possess multiple genes for both the decapeptide gonadotropin releasing hormone (GnRH) and for the GnRH receptor. The evolutionary history of the receptor family, including ancestral copy number and timing of duplications and deletions, has been the subject of controversy.
  
  
  RESULTS
  We report here for the first time sequences of three distinct GnRH receptor genes in salamanders (axolotls, Ambystoma mexicanum), which are orthologous to three GnRH receptors from ranid frogs. To understand the origin of these genes within the larger evolutionary context of the gene family, we performed phylogenetic analyses and probabilistic protein homology searches of GnRH receptor genes in vertebrates and their near relatives. Our analyses revealed four points that alter previous views about the evolution of the GnRH receptor gene family. First, the "mammalian" pituitary type GnRH receptor, which is the sole GnRH receptor in humans and previously presumed to be highly derived because it lacks the cytoplasmic C-terminal domain typical of most G-protein coupled receptors, is actually an ancient gene that originated in the common ancestor of jawed vertebrates (Gnathostomata). Second, unlike previous studies, we classify vertebrate GnRH receptors into five subfamilies. Third, the order of subfamily origins is the inverse of previous proposed models. Fourth, the number of GnRH receptor genes has been dynamic in vertebrates and their ancestors, with multiple duplications and losses.
  
  
  CONCLUSIONS
  Our results provide a novel evolutionary framework for generating hypotheses concerning the functional importance of structural characteristics of vertebrate GnRH receptors. We show that five subfamilies of vertebrate GnRH receptors evolved early in the vertebrate phylogeny, followed by several independent instances of gene loss. Chief among cases of gene loss are humans, best described as degenerate with respect to GnRH receptors because we retain only a single, ancient gene.
authors.forename: Barry L
authors.initials: BL
authors.lastname: Williams
authors.forename: Yasuhisa
authors.initials: Y
authors.lastname: Akazome
authors.forename: Yoshitaka
authors.initials: Y
authors.lastname: Oka
authors.forename: Heather L
authors.initials: HL
authors.lastname: Eisthen
mesh_terms: D000558:Ambystoma mexicanum / Q000235:genetics*; D000595:Amino Acid Sequence; D029845:Amphibian Proteins / Q000235:genetics*; D000818:Animals; D001483:Base Sequence; D019143:Evolution, Molecular; D007987:Gonadotropin-Releasing Hormone; D008969:Molecular Sequence Data; D010802:Phylogeny; D011966:Receptors, LHRH / Q000235:genetics*; D016415:Sequence Alignment; D014714:Vertebrates / Q000235:genetics
chemical_list: D029845:Amphibian Proteins; D011966:Receptors, LHRH; D007987:Gonadotropin-Releasing Hormone