title: Investigation of biotechnological potential of sponge-associated bacteria collected in Brazilian coast.
abstract: Marine bacteria are a rich source of structurally unique natural compounds, several of which have shown a wide variety of biological activities. In this study, the metabolites present in the culture supernatants of the eight sponge-associated bacteria were extracted using ethyl acetate, and all extracts showed activity against Staphylococcus aureus. Subsequently, the extracts of the Pseudomonas fluorescens H40 and H41, and Pseudomonas aeruginosa H51 were subjected to solvent partitioning, and the active fractions were submitted to chromatographic separation. Three different active fractions were obtained, one of which was identified as diketopiperazine cyclo-(L-Leu-L-Pro). This substance was bactericidal for Staph. aureus and Ps. aeruginosa and showed cytotoxic activity against HEp-2 tumour cells. Putative gene fragments coding for the type I polyketide synthase (PKS-I) and nonribosomal peptide synthetase (NRPS) domains were PCR-amplified from five and three strains, respectively. The results suggest that sponge-associated bacteria analysed in this study may represent a potential source for production of antimicrobial substances against bacterial pathogens of medical importance.
authors.affiliation: Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.
authors.forename: O C S
authors.initials: OCS
authors.lastname: Santos
authors.affiliation: Grupo de Produtos Naturais de Organismos Aquáticos (GPNOA), Núcleo em Ecologia e Desenvolvimento Sócio-Ambiental de Macaé, Universidade Federal do Rio de Janeiro, Macaé, Rio de Janeiro, Brazil.
authors.forename: A R
authors.initials: AR
authors.lastname: Soares
authors.affiliation: Grupo de Produtos Naturais de Organismos Aquáticos (GPNOA), Núcleo em Ecologia e Desenvolvimento Sócio-Ambiental de Macaé, Universidade Federal do Rio de Janeiro, Macaé, Rio de Janeiro, Brazil.
authors.forename: F L S
authors.initials: FLS
authors.lastname: Machado
authors.affiliation: Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.
authors.forename: M T V
authors.initials: MTV
authors.lastname: Romanos
authors.affiliation: Museu Nacional, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.
authors.forename: G
authors.initials: G
authors.lastname: Muricy
authors.affiliation: Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.
authors.forename: M
authors.initials: M
authors.lastname: Giambiagi-deMarval
authors.affiliation: Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.
authors.forename: M S
authors.initials: MS
authors.lastname: Laport
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000096:biosynthesis* / Q000737:chemistry / Q000494:pharmacology*; D000898:Antibiosis; D001419:Bacteria / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D001709:Biotechnology; D001938:Brazil; D045744:Cell Line, Tumor; D054659:Diketopiperazines / Q000378:metabolism; D008826:Microbial Sensitivity Tests; D010453:Peptide Synthases / Q000235:genetics; D048630:Polyketide Synthases / Q000235:genetics; D011161:Porifera / Q000382:microbiology*; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000302:isolation & purification / Q000378:metabolism; D011551:Pseudomonas fluorescens / Q000302:isolation & purification / Q000378:metabolism; D013211:Staphylococcus aureus / Q000187:drug effects
chemical_list: D000900:Anti-Bacterial Agents; D054659:Diketopiperazines; D048630:Polyketide Synthases; D010453:Peptide Synthases; C104385:non-ribosomal peptide synthase
keywords: Pseudomonas sp; diketopiperazine; marine sponges; nonribosomal peptide synthetase; polyketide synthase