title: Use of methacrylate-modified chitosan to increase the durability of dentine bonding systems.
abstract: This study aimed at investigating the effect of a methacrylate-modified chitosan on the durability of adhesive interfaces to improve the clinical performance of dental restorations. Chitosan was modified with methacrylic acid (Chit-MA70) on 16% of the amino groups. Viscosity, rheology, and (1)H NMR spectroscopy were performed to characterize the modified polysaccharide. Chit-MA70 was blended into a primer of an "etch-and-rinse" experimental adhesive system and tested on human teeth. The presence of methacrylate moieties and of residual positive charges on the polysaccharide chain allowed Chit-MA70 to covalently bind to the restorative material and electrostatically interact with demineralized dentin. The Chit-MA70 containing an adhesive system showed values of the immediate bond strength (26.0 ± 8.7 MPa) comparable to the control adhesive system (25.5 ± 8.7 MPa). However, it was shown that upon performing thermo-mechanical cycling treatment of the dental restoration on human teeth, the adhesive with the methacrylate-modified chitosan, in variance with the control adhesive, did not show any decrease in the bond strength (28.4 ± 8.8 MPa). The modified chitosan is proposed as a component of the "etch-and-rinse" adhesive system to efficiently improve the durability of dental restorations.
authors.affiliation: Department of Medical, Surgical, and Health Sciences, University of Trieste , Piazza dell'Ospitale 1, 34129 Trieste, Italy.
authors.forename: Marina
authors.initials: M
authors.lastname: Diolosà
authors.forename: Ivan
authors.initials: I
authors.lastname: Donati
authors.forename: Gianluca
authors.initials: G
authors.lastname: Turco
authors.forename: Milena
authors.initials: M
authors.lastname: Cadenaro
authors.forename: Roberto
authors.initials: R
authors.lastname: Di Lenarda
authors.forename: Lorenzo
authors.initials: L
authors.lastname: Breschi
authors.forename: Sergio
authors.initials: S
authors.lastname: Paoletti
mesh_terms: D048271:Chitosan / Q000737:chemistry*; D003188:Composite Resins / Q000737:chemistry; D001840:Dental Bonding; D003804:Dentin / Q000737:chemistry*; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D008689:Methacrylates / Q000737:chemistry*; D018613:Microscopy, Confocal; D008855:Microscopy, Electron, Scanning; D008963:Molar; D058105:Polymerization; D013048:Specimen Handling; D013055:Spectrophotometry, Infrared; D013499:Surface Properties; D013718:Tensile Strength
chemical_list: D003188:Composite Resins; D008689:Methacrylates; C008384:methacrylic acid; D048271:Chitosan