title: Ultra small angle x-ray scattering in complex mixtures of triacylglycerols.
abstract: Ultra-small angle x-ray scattering (USAXS) has been used to elucidate, in situ, the aggregation structure of unsheared model edible oils. Each system comprised one or two solid lipids and a combination of liquid lipids. The 3D nano- to micro-structures of each system were characterized. The length scale investigated, using the Bonse-Hart camera at beamline ID-15D at the Advanced Photon Source, ANL, ranged from 300 Å-10 µm. Using the Unified Fit model, level-1 analysis showed that the scatterers were 2D objects with either a smooth, a rough, or a diffuse surface. These 2D objects had an average radius of gyration Rg1 between 200-1500 Å. Level-2 analysis displayed a slope between -1 and -2. Use of the Guinier-Porod model gave s ≈ 1 thus showing that it was cylinders (TAGwoods) aggregating with fractal dimension 1 ≤ D2 ≤ 2. D2 = 1 is consistent with 1D structures formed from TAGwoods, while D2 = 2 implies that the TAGwoods had formed structures characteristic of diffusion or reaction limited cluster-cluster aggregation (DLCA/RLCA). These aggregates exhibited radii of gyration, Rg2, between 2500 and 6500 Å. Level-3 analyses showed diffuse surfaces, for most of the systems. These interpretations are in accord with theoretical models which studied crystalline nano-platelets (CNPs) coated with nano-scale layers arising from phase separation at the CNP surfaces. These layers could be due to either liquid-liquid phase separation with the CNPs coated, uniformly or non-uniformly, by a diffuse layer of TAGs, or solid-liquid phase separation with the CNPs coated by a rough layer of crystallites.A fundamental understanding of the self-organizing structures arising in these systems helps advance the characterization of fat crystal networks from nanometres to micrometres. This research can be used to design novel fat structures that use healthier fats via nano- and meso-scale structural engineering.
authors.affiliation: Food Science Department, University of Guelph, Guelph, ON, Canada.
authors.forename: Fernanda
authors.initials: F
authors.lastname: Peyronel
authors.forename: Bonnie
authors.initials: B
authors.lastname: Quinn
authors.forename: Alejandro G
authors.initials: AG
authors.lastname: Marangoni
authors.forename: David A
authors.initials: DA
authors.lastname: Pink
mesh_terms: D049329:Nanostructures / Q000737:chemistry*; D044367:Phase Transition; D053838:Scattering, Small Angle; D014304:Triolein / Q000737:chemistry*; D014961:X-Ray Diffraction
chemical_list: D014304:Triolein