title: Effect of the fatty acid composition of acclimated oenological Lactobacillus plantarum on the resistance to ethanol.
abstract: The aim of this work was to evaluate the changes due to acclimation to ethanol on the fatty acid composition of three oenological Lactobacillus plantarum strains and their effect on the resistance to ethanol and malic acid consumption (MAC). Lactobacillus plantarum UNQLp 133, UNQLp 65.3 and UNQLp 155 were acclimated in the presence of 6 or 10% v/v ethanol, for 48 h at 28°C. Lipids were extracted to obtain fatty acid methyl esters and analysed by gas chromatography interfaced with mass spectroscopy. The influence of change in fatty acid composition on the viability and MAC in synthetic wine was analysed by determining the Pearson correlation coefficient. Acclimated strains showed a significant change in the fatty composition with regard to the nonacclimated strains. Adaptation to ethanol led to a decrease in the unsaturated/saturated ratio, mainly resulting from an increase in the contribution of short-length fatty acid C12:0 and a decrease of C18:1. The content of C12:0 was related to a higher viability after inoculation of synthetic wine. The MAC increased at higher contents in saturated fatty acid, but its efficiency was strain dependent.
authors.affiliation: Laboratorio de Microbiología Molecular, Departamento de Ciencia y Tecnología, Universidad Nacional de Quilmes, Bernal, Argentina.
authors.forename: B M
authors.initials: BM
authors.lastname: Bravo-Ferrada
authors.affiliation: Centro de Investigación y Desarrollo en Criotecnología de Alimentos (CIDCA) (CONICET La Plata, UNLP), La Plata, Argentina.
authors.forename: A
authors.identifier: http://orcid.org/0000-0002-8705-0160
authors.initials: A
authors.lastname: Gómez-Zavaglia
authors.affiliation: Laboratorio de Microbiología Molecular, Departamento de Ciencia y Tecnología, Universidad Nacional de Quilmes, Bernal, Argentina.
authors.forename: L
authors.initials: L
authors.lastname: Semorile
authors.affiliation: Laboratorio de Microbiología Molecular, Departamento de Ciencia y Tecnología, Universidad Nacional de Quilmes, Bernal, Argentina.
authors.forename: E E
authors.initials: EE
authors.lastname: Tymczyszyn
mesh_terms: D000222:Adaptation, Physiological; D002849:Chromatography, Gas; D024881:Drug Resistance, Bacterial; D000431:Ethanol / Q000032:analysis / Q000494:pharmacology*; D005227:Fatty Acids / Q000032:analysis*; D005231:Fatty Acids, Unsaturated / Q000032:analysis; D048191:Lactiplantibacillus plantarum / Q000737:chemistry* / Q000187:drug effects*; D008293:Malates / Q000032:analysis*; D050296:Microbial Viability; D014920:Wine / Q000382:microbiology*
chemical_list: D005227:Fatty Acids; D005231:Fatty Acids, Unsaturated; D008293:Malates; D000431:Ethanol; C030298:malic acid
keywords: Lactobacillus plantarum; acclimation; ethanol stress; fatty acid; wine