title: Aquaporin 5 expression is altered in ovarian tumors and ascites-derived ovarian tumor cells in the chicken model of ovarian tumor.
abstract: BACKGROUND
  Aquaporin 5 (AQP5), a member of the aquaporin family of transmembrane channel proteins, is involved in water transport and cellular proliferation in various tumors. The objective of this study was to determine cellular localization of aquaporin 5 (AQP5) in the ovarian tumors of chicken, a preclinical model for human ovarian tumor and to determine if AQP5 mRNA and protein expression levels in cancerous chicken ovaries and in ascites-derived chicken ovarian cancer (COVCAR) cell lines are different from normal ovaries and normal ovarian surface epithelial (NOSE) cells, respectively.
  
  
  METHODS
  Immunohistochemical staining was performed to determine the localization of AQP5-immunoreactive (ir) cells in normal and cancerous ovaries. To determine AQP5 mRNA and protein concentrations in cancerous ovaries and COVCAR cell lines, quantitative real time PCR and Western blotting analysis were performed, respectively. Student's t-test was performed to compare the levels of AQP5 mRNA or protein in cancerous ovaries and COVCAR cell lines with that of normal ovaries and NOSE cells, respectively.
  
  
  RESULTS
  AQP5-ir cells were localized in granulosa and theca layers of normal ovarian follicles whereas cancerous ovaries showed AQP5 immunostaining in the surface epithelium, fibroblast cells of the stroma, and in the cells lining tumor cysts and acini. AQP5 mRNA concentration were significantly lesser while AQP5 protein concentrations were significantly greater in cancerous ovaries compared to that in normal ovaries (P < 0.05). Whereas AQP5 mRNA concentrations were significantly greater while AQP5 protein concentrations were lesser (P < 0.05) in COVCAR cell lines compared with that in NOSE cells.
  
  
  CONCLUSIONS
  AQP5 is differentially expressed in ovarian tumor and in COVCAR cell lines suggesting a potential involvement of AQP5 in ovarian tumorigenesis, metastasis, and survival of ovarian tumor cells in ascites.
authors.forename: Anupama
authors.initials: A
authors.lastname: Tiwari
authors.forename: Jill A
authors.initials: JA
authors.lastname: Hadley
authors.forename: Ramesh
authors.initials: R
authors.lastname: Ramachandran
mesh_terms: D000818:Animals; D051402:Aquaporin 5 / Q000235:genetics* / Q000378:metabolism*; D001201:Ascites / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D030161:Avian Proteins / Q000235:genetics* / Q000378:metabolism*; D045744:Cell Line, Tumor; D002645:Chickens; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D009374:Neoplasms, Experimental / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D010051:Ovarian Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*
chemical_list: D051402:Aquaporin 5; D030161:Avian Proteins