title: The effect of n-acetylcysteine on hepatic histomorphology during hypothermic preservation.
abstract: OBJECTIVE
  To evaluate the NAC effects on liver hypothermic preservation at different time intervals.
  
  
  METHODS
  For this, we used livers of male Wistar rats weighing between 250 and 300 g, undergoing preservation in Ringer solution at 4°C for up to 24 hours. Tissue samples were obtained at four moments of preservation for histological examination by hematoxylin and eosin staining: T0 = beginning of preservation, T12 = 12 hours, T18 = 18 hours and T24 = 24 hours. Will be analyzed vacuolation, hepatic apoptosis by optical microscopy and parenchymal.
  
  
  RESULTS
  The results showed a progressive increase in hepatic injury in both groups and showed that NAC was effective at T0. The parenchyma preservation was better in the NAC group and no difference when vacuolization of the cells.
  
  
  CONCLUSIONS
  Hypothermic preservation, over time, causes changes in the hepatic parenchyma with increased apoptosis, loss of architecture, vacuolization, culminating in severe injury. The administration of N-acetylcysteine protects against preservation liver injury.
authors.affiliation: University City of São Paulo, São Paulo, Brazil.
authors.forename: Patricia Sayuri
authors.initials: PS
authors.lastname: Risso
authors.affiliation: Medical School, São Paulo University, São Paulo, Brazil.
authors.forename: Marcia Kiyomi
authors.initials: MK
authors.lastname: Koike
authors.affiliation: Paulista Medicine School, Federal University of São Paulo, São Paulo, Brazil.
authors.forename: Marcos de Souza
authors.initials: Mde S
authors.lastname: Abrahão
authors.affiliation: Medical School, São Paulo University, São Paulo, Brazil.
authors.forename: Natalie Chaves
authors.initials: NC
authors.lastname: Ferreira
authors.affiliation: Medical School, São Paulo University, São Paulo, Brazil.
authors.forename: Edna Frasson de Souza
authors.initials: EF
authors.lastname: Montero
mesh_terms: D000111:Acetylcysteine / Q000494:pharmacology*; D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D015925:Cryopreservation / Q000379:methods*; D016166:Free Radical Scavengers / Q000494:pharmacology*; D008099:Liver / Q000033:anatomy & histology / Q000187:drug effects*; D008297:Male; D023421:Models, Animal; D009926:Organ Preservation / Q000009:adverse effects / Q000379:methods*; D011897:Random Allocation; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000517:prevention & control; D013997:Time Factors
chemical_list: D016166:Free Radical Scavengers; D000111:Acetylcysteine