title: Na⁺, K⁺-ATPase and Ca²⁺-ATPase activities in basal and microvillous syncytiotrophoblast membranes from preeclamptic human term placenta.
abstract: OBJECTIVE
  The aim of this study is to evaluate the effect of preeclampsia on the level of lipid peroxidation, activity and expression of both plasma membrane Ca(2+)- and Na(+), K(+)-ATPases in syncytiotrophoblast.
  
  
  METHODS
  The level of lipid peroxidation was estimated by measuring TBARS. ATPase activities were quantified by a colorimetric method measuring the amount of inorganic phosphate during the assay. Expression of Ca(2+)- and Na(+), K(+)-ATPases in syncytiotrophoblast plasma membranes and term placenta tissue sections was investigated using Western blot and immunohistochemistry, respectively.
  
  
  RESULTS
  Our results show a higher level of lipid peroxidation of syncytiotrophoblast plasma membranes from preeclamptic, as compared to uncomplicated pregnant women. Preeclampsia also significantly reduced the activity of Ca(2+)- and Na(+), K(+)-ATPases; however, expression of both ATPases was unaffected.
  
  
  CONCLUSIONS
  Our findings suggest that the reduction of Ca(2+)- and Na(+), K(+)-ATPase activities during preeclampsia could be at least partially due to an increased level of lipid peroxidation of the syncytiotrophoblast plasma membranes.
authors.affiliation: Laboratorio de Bioenergética Celular, Centro de Biofísica y Bioquímica, Instituto Venezolano de Investigaciones Científicas (IVIC) , Caracas , Venezuela , and.
authors.forename: Cilia
authors.initials: C
authors.lastname: Abad
authors.forename: Catalina
authors.initials: C
authors.lastname: Vallejos
authors.forename: Nicole
authors.initials: N
authors.lastname: De Gregorio
authors.forename: Paula
authors.initials: P
authors.lastname: Díaz
authors.forename: Delia I
authors.initials: DI
authors.lastname: Chiarello
authors.forename: Mariana
authors.initials: M
authors.lastname: Mendoza
authors.forename: Sandy
authors.initials: S
authors.lastname: Piñero
authors.forename: Teresa
authors.initials: T
authors.lastname: Proverbio
authors.forename: Desirée
authors.initials: D
authors.lastname: Botana
authors.forename: Próspero
authors.initials: P
authors.lastname: Rojas
authors.forename: Gloria
authors.initials: G
authors.lastname: Riquelme
authors.forename: Fulgencio
authors.initials: F
authors.lastname: Proverbio
authors.forename: Reinaldo
authors.initials: R
authors.lastname: Marín
mesh_terms: D000328:Adult; D015153:Blotting, Western; D000252:Calcium-Transporting ATPases / Q000378:metabolism*; D015331:Cohort Studies; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D015227:Lipid Peroxidation; D010920:Placenta / Q000378:metabolism*; D011225:Pre-Eclampsia / Q000378:metabolism*; D011247:Pregnancy; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism*; D055815:Young Adult
chemical_list: D000252:Calcium-Transporting ATPases; D000254:Sodium-Potassium-Exchanging ATPase
keywords: Ca2+-ATPase; Na+, K+-ATPase; Placenta; Preeclampsia; Syncytiotrophoblast