title: Pri peptides are mediators of ecdysone for the temporal control of development.
abstract: Animal development fundamentally relies on the precise control, in space and time, of genome expression. Whereas we have a wealth of information about spatial patterning, the mechanisms underlying temporal control remain poorly understood. Here we show that Pri peptides, encoded by small open reading frames, are direct mediators of the steroid hormone ecdysone for the timing of developmental programs in Drosophila. We identify a previously uncharacterized enzyme of ecdysone biosynthesis, GstE14, and find that ecdysone triggers pri expression to define the onset of epidermal trichome development, through post-translational control of the Shavenbaby transcription factor. We show that manipulating pri expression is sufficient to either put on hold or induce premature differentiation of trichomes. Furthermore, we find that ecdysone-dependent regulation of pri is not restricted to epidermis and occurs over various tissues and times. Together, these findings provide a molecular framework to explain how systemic hormonal control coordinates specific programs of differentiation with developmental timing.
authors.affiliation: 1] Centre de Biologie du Développement, Université de Toulouse, UPS, 31062 Toulouse cedex 9, France [2] CNRS, UMR5547, Centre de Biologie du Développement, Toulouse, 31062 cedex 9, France.
authors.forename: Hélène
authors.initials: H
authors.lastname: Chanut-Delalande
authors.affiliation: Okazaki Institute for Integrative Bioscience, National institutes of Natural Sciences Okazaki, Aichi 444-8787, Japan.
authors.forename: Yoshiko
authors.initials: Y
authors.lastname: Hashimoto
authors.affiliation: 1] Centre de Biologie du Développement, Université de Toulouse, UPS, 31062 Toulouse cedex 9, France [2] CNRS, UMR5547, Centre de Biologie du Développement, Toulouse, 31062 cedex 9, France.
authors.forename: Anne
authors.initials: A
authors.lastname: Pelissier-Monier
authors.affiliation: Institute for Genomics and Systems Biology and Department of Human Genetics, University of Chicago, Illinois 60637, USA.
authors.forename: Rebecca
authors.initials: R
authors.lastname: Spokony
authors.affiliation: 1] Centre de Biologie du Développement, Université de Toulouse, UPS, 31062 Toulouse cedex 9, France [2] CNRS, UMR5547, Centre de Biologie du Développement, Toulouse, 31062 cedex 9, France.
authors.forename: Azza
authors.initials: A
authors.lastname: Dib
authors.affiliation: Okazaki Institute for Integrative Bioscience, National institutes of Natural Sciences Okazaki, Aichi 444-8787, Japan.
authors.forename: Takefumi
authors.initials: T
authors.lastname: Kondo
authors.affiliation: 1] Centre de Biologie du Développement, Université de Toulouse, UPS, 31062 Toulouse cedex 9, France [2] CNRS, UMR5547, Centre de Biologie du Développement, Toulouse, 31062 cedex 9, France.
authors.forename: Jérôme
authors.initials: J
authors.lastname: Bohère
authors.affiliation: Research Center for Environmental Genomics, Organization of Advanced Science and Technology, Kobe University, Kobe 657-850, Japan.
authors.forename: Kaori
authors.initials: K
authors.lastname: Niimi
authors.affiliation: 1] Centre de Biologie du Développement, Université de Toulouse, UPS, 31062 Toulouse cedex 9, France [2] CNRS, UMR5547, Centre de Biologie du Développement, Toulouse, 31062 cedex 9, France.
authors.forename: Yvan
authors.initials: Y
authors.lastname: Latapie
authors.affiliation: Research Center for Environmental Genomics, Organization of Advanced Science and Technology, Kobe University, Kobe 657-850, Japan.
authors.forename: Sachi
authors.initials: S
authors.lastname: Inagaki
authors.affiliation: 1] Centre de Biologie du Développement, Université de Toulouse, UPS, 31062 Toulouse cedex 9, France [2] CNRS, UMR5547, Centre de Biologie du Développement, Toulouse, 31062 cedex 9, France.
authors.forename: Laurence
authors.initials: L
authors.lastname: Dubois
authors.affiliation: 1] Centre de Biologie du Développement, Université de Toulouse, UPS, 31062 Toulouse cedex 9, France [2] CNRS, UMR5547, Centre de Biologie du Développement, Toulouse, 31062 cedex 9, France.
authors.forename: Philippe
authors.initials: P
authors.lastname: Valenti
authors.affiliation: 1] Centre de Biologie du Développement, Université de Toulouse, UPS, 31062 Toulouse cedex 9, France [2] CNRS, UMR5547, Centre de Biologie du Développement, Toulouse, 31062 cedex 9, France.
authors.forename: Cédric
authors.initials: C
authors.lastname: Polesello
authors.affiliation: Okazaki Institute for Integrative Bioscience, National institutes of Natural Sciences Okazaki, Aichi 444-8787, Japan.
authors.forename: Satoru
authors.initials: S
authors.lastname: Kobayashi
authors.affiliation: Animal Genetics, Interfaculty Institute for Cell Biology, University of Tübingen, 72076 Tübingen, Germany.
authors.forename: Bernard
authors.initials: B
authors.lastname: Moussian
authors.affiliation: Institute for Genomics and Systems Biology and Department of Human Genetics, University of Chicago, Illinois 60637, USA.
authors.forename: Kevin P
authors.initials: KP
authors.lastname: White
authors.affiliation: 1] Centre de Biologie du Développement, Université de Toulouse, UPS, 31062 Toulouse cedex 9, France [2] CNRS, UMR5547, Centre de Biologie du Développement, Toulouse, 31062 cedex 9, France.
authors.forename: Serge
authors.initials: S
authors.lastname: Plaza
authors.affiliation: 1] Research Center for Environmental Genomics, Organization of Advanced Science and Technology, Kobe University, Kobe 657-850, Japan [2] Department of Biology, Graduate School of Science, Kobe University, Kobe 657-850, Japan.
authors.forename: Yuji
authors.initials: Y
authors.lastname: Kageyama
authors.affiliation: 1] Centre de Biologie du Développement, Université de Toulouse, UPS, 31062 Toulouse cedex 9, France [2] CNRS, UMR5547, Centre de Biologie du Développement, Toulouse, 31062 cedex 9, France.
authors.forename: François
authors.initials: F
authors.lastname: Payre
mesh_terms: D000818:Animals; D019390:Arrestins / Q000235:genetics / Q000378:metabolism*; D002454:Cell Differentiation / Q000235:genetics; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000378:metabolism*; D004440:Ecdysone / Q000235:genetics / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D005982:Glutathione Transferase / Q000235:genetics / Q000378:metabolism*; D009154:Mutation / Q000235:genetics; D011987:Receptors, Steroid / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction / Q000502:physiology; D014153:Transaldolase / Q000235:genetics / Q000378:metabolism
chemical_list: C504666:Arr2 protein, Drosophila; D019390:Arrestins; D029721:Drosophila Proteins; D011987:Receptors, Steroid; C055469:ecdysone receptor; D004440:Ecdysone; C523787:Tal protein, Drosophila; D014153:Transaldolase; D005982:Glutathione Transferase; C000602619:Gste14 protein, Drosophila