title: TRIF Deficiency does not Affect Severity of Ovalbumin-induced Airway Inflammation in Mice.
abstract: Allergic asthma is a chronic pulmonary inflammatory disease characterized by reversible airway obstruction, hyperresponsiveness and eosinophils infiltration. Toll-like receptors (TLRs) signaling are closely associated with asthma and have emerged as a novel therapeutic target in allergic disease. The functions of TLR3 and TLR4 in allergic airway inflammation have been studied; however, the precise role of TIR-domain-containing adapter-inducing interferon-β (TRIF), the adaptor molecule for both TLR3 and TLR4, is not yet fully understood. To investigate this, we developed a mouse model of OVA-induced allergic airway inflammation and compared the severity of allergic airway inflammation in WT and TRIF(-/-) mice. Histopathological assessment revealed that the severity of inflammation in airway inflammation in TRIF-deficient mice was comparable to that in WT mice. The total number of cells recovered from bronchoalveolar lavage fluid did not differ between WT and TRIF-deficient mice. Moreover, TRIF deficiency did not affect Th1 and Th2 cytokine production in lung tissue nor the level of serum OVA-specific IgE, IgG1 and IgG2c. These findings suggest that TRIF-mediated signaling may not be critical for the development of allergic airway inflammation.
authors.affiliation: Laboratory Animal Medicine, College of Veterinary Medicine, Seoul National University, Seoul 151-742, Korea.
authors.forename: Tae-Hyoun
authors.initials: TH
authors.lastname: Kim
authors.affiliation: Department of Biochemistry, College of Medicine, Konyang University, Daejeon 302-718, Korea.
authors.forename: Dong-Jae
authors.initials: DJ
authors.lastname: Kim
authors.affiliation: Laboratory Animal Medicine, College of Veterinary Medicine, Seoul National University, Seoul 151-742, Korea.
authors.forename: Jae-Hak
authors.initials: JH
authors.lastname: Park
authors.affiliation: Department of Laboratory Animal Medicine, College of Veterinary Medicine, Chonnam National University, Gwangju 500-757, Korea.
authors.forename: Jong-Hwan
authors.initials: JH
authors.lastname: Park
keywords: Allergic airway inflammation; TRIF; Th2