title: Rapid identification of Stenotrophomonas maltophilia by peptide nucleic acid fluorescence in situ hybridization.
abstract: The objective of this study was to develop a novel peptide nucleic acid (PNA) probe for Stenotrophomonas maltophilia identification by fluorescence in situ hybridization (FISH). The probe was evaluated using 33 human and veterinary clinical S. maltophilia isolates and 45 reference strains representing common bacterial species in the respiratory tract. The probe displayed 100% sensitivity and 100% specificity on pure cultures and allowed detection in sputum from cystic fibrosis patients. The detection limit was 10(4) CFU/mL in spiked tracheal aspirate and bronchoalveolar lavage from healthy horses. Altogether the study shows that this species-specific PNA FISH probe facilitates rapid detection of S. maltophilia in biological specimens.
authors.affiliation: Department of Veterinary Disease Biology, Faculty of Health and Medical Sciences, University of Copenhagen Stigbøjlen, Frederiksberg, Denmark.
authors.forename: N
authors.initials: N
authors.lastname: Hansen
authors.affiliation: Dako Denmark A/S Produktionsvej, Glostrup, Denmark.
authors.forename: A K I
authors.initials: AK
authors.lastname: Rasmussen
authors.affiliation: AdvanDx Inc. Woburn, MA, USA.
authors.forename: M J
authors.initials: MJ
authors.lastname: Fiandaca
authors.affiliation: Department for International Health, Immunology and Microbiology, Faculty of Health and Medical Sciences, University of Copenhagen Copenhagen, Denmark.
authors.forename: K N
authors.initials: KN
authors.lastname: Kragh
authors.affiliation: Department for Clinical Microbiology, University Hospital of Copenhagen Juliane Mariesvej, Copenhagen, Denmark ; Department for International Health, Immunology and Microbiology, Faculty of Health and Medical Sciences, University of Copenhagen Copenhagen, Denmark.
authors.forename: T
authors.initials: T
authors.lastname: Bjarnsholt
authors.affiliation: Department for Clinical Microbiology, University Hospital of Copenhagen Juliane Mariesvej, Copenhagen, Denmark ; Department for International Health, Immunology and Microbiology, Faculty of Health and Medical Sciences, University of Copenhagen Copenhagen, Denmark.
authors.forename: N
authors.initials: N
authors.lastname: Høiby
authors.affiliation: Stender Diagnostics Gentofte, Denmark.
authors.forename: H
authors.initials: H
authors.lastname: Stender
authors.affiliation: Department of Veterinary Disease Biology, Faculty of Health and Medical Sciences, University of Copenhagen Stigbøjlen, Frederiksberg, Denmark.
authors.forename: L
authors.initials: L
authors.lastname: Guardabassi
keywords: Cystic fibrosis; PNA FISH; Stenotrophomonas maltophilia; identification