title: Fixation methods for the preservation of morphology, RNAs, and proteins in paraffin-embedded human cervical cancer cell xenografts in mice.
abstract: After various types of fixation, paraffin-embedded tissues are commonly used for histological analysis and pathological diagnosis; they are also suitable for long-term storage. Neutral buffered formalin, paraformaldehyde, and ethanol are common fixatives for histopathological analysis. For molecular biological analysis, fixed paraffin-embedded tissues are valuable resources; suitable fixative solutions and methods are needed to quantify and perform molecular biological analyses including immunohistochemistry, in situ hybridization, and quantitative polymerase chain reaction. Currently, 4 % paraformaldehyde is the recommended fixative for the preservation of RNAs and proteins, as well as for morphological study in paraffin-embedded human cervical cancer tissues that were xenografted in immunodeficient mice. Here, we describe the method for the fixation and preparation of paraffin-embedded tissue specimens for analysis of RNAs, proteins, and morphology.
authors.affiliation: Department of Pathology, Tokyo Metropolitan Geriatric Hospital and Institute of Gerontology, 35-2 Sakae-cho, Itabashi-ku, Tokyo, 173-0015, Japan.
authors.forename: Yoko
authors.initials: Y
authors.lastname: Matsuda
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Ishiwata
mesh_terms: D000818:Animals; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D051379:Mice; D009363:Neoplasm Proteins / Q000378:metabolism*; D016612:Paraffin Embedding / Q000379:methods*; D012334:RNA, Neoplasm / Q000302:isolation & purification / Q000378:metabolism*; D016707:Tissue Fixation / Q000379:methods*; D002583:Uterine Cervical Neoplasms / Q000473:pathology*; D023041:Xenograft Model Antitumor Assays
chemical_list: D009363:Neoplasm Proteins; D012334:RNA, Neoplasm