title: Dietary fat modulates dl-α-tocopheryl acetate (vitamin E) bioavailability in adult cockerels.
abstract: 1. A trial was designed to assess the effect of fat supplementation (amount and type of fatty acids) on vitamin E bioavailability in adult cockerels. 2. A total of 60 birds were force-fed three different diets: a semi-purified diet without added fat (Control diet) or supplemented with 3% fat as linseed (Linseed diet) or hydrogenated coconut oil (Coconut diet). The three experimental diets were also supplemented with dl-α-tocopheryl acetate to provide 40 mg vitamin E per bird. 3. After one week of depletion, blood was collected from the wing vein before (baseline) and 6, 12, 24 and 96 h after the gavage. Plasma samples were analysed for their α-tocopherol, cholesterol and triglycerides concentrations. 4. Results showed that the addition of 3% fat in the experimental diet increased post-gavage plasma α-tocopherol response by 153% for Linseed diet and by 75% for Coconut diet (P < 0.0001) compared to the Control group. Furthermore, the plasma α-tocopherol response observed with the Linseed diet was 44% greater than that observed with the Coconut diet (P < 0.0001). There was no effect of treatments on either plasma triglycerides (P = 0.91) or cholesterol (P = 0.45) responses. 5. In conclusion, this study shows that the addition of 3% fat to the diet significantly increases dl-α-tocopheryl acetate bioavailability in adult cockerels. Supplementation of fat rich in unsaturated fatty acids also leads to a higher dl-α-tocopheryl acetate bioavailability than fat rich in saturated fatty acids.
authors.affiliation: a Centre of Expertise and Research in Nutrition , Adisseo France SAS , Commentry , France.
authors.forename: D P
authors.initials: DP
authors.lastname: Prévéraud
authors.forename: E
authors.initials: E
authors.lastname: Devillard
authors.forename: P
authors.initials: P
authors.lastname: Borel
mesh_terms: D000821:Animal Feed / Q000032:analysis; D000818:Animals; D001682:Biological Availability; D002645:Chickens / Q000378:metabolism*; D002784:Cholesterol / Q000097:blood; D004032:Diet / Q000662:veterinary*; D004041:Dietary Fats / Q000008:administration & dosage / Q000378:metabolism*; D019587:Dietary Supplements / Q000032:analysis; D004305:Dose-Response Relationship, Drug; D004750:Enteral Nutrition / Q000662:veterinary; D008297:Male; D011897:Random Allocation; D014280:Triglycerides / Q000097:blood; D014810:Vitamin E / Q000493:pharmacokinetics*; D024502:alpha-Tocopherol / Q000097:blood
chemical_list: D004041:Dietary Fats; D014280:Triglycerides; D014810:Vitamin E; D002784:Cholesterol; D024502:alpha-Tocopherol