title: Longitudinal changes in resting-state brain activity in a capsular infarct model.
abstract: Strokes attributable to subcortical infarcts have been increasing recently in elderly patients. To gain insight how this lesion influences the motor outcome and responds to rehabilitative training, we used circumscribed photothrombotic capsular infarct models on 36 Sprague-Dawley rats (24 experimental and 12 sham-operated). We used 2-deoxy-2-[(18)F]-fluoro-D-glucose-micro positron emission tomography (FDG-microPET) to assess longitudinal changes in resting-state brain activity (rs-BA) and daily single-pellet reaching task (SPRT) trainings to evaluate motor recovery. Longitudinal FDG-microPET results showed that capsular infarct resulted in a persistent decrease in rs-BA in bilateral sensory and auditory cortices, and ipsilesional motor cortex, thalamus, and inferior colliculus (P<0.0025, false discovery rate (FDR) q<0.05). The decreased rs-BA is compatible with diaschisis and contributes to manifest the malfunctions of lesion-specific functional connectivity. In contrast, capsular infarct resulted in increase of rs-BA in the ipsilesional internal capsule, and contralesional red nucleus and ventral hippocampus in recovery group (P<0.0025, FDR q<0.05), implying that remaining subcortical structures have an important role in conducting the recovery process in capsular infarct. The SPRT training facilitated motor recovery only in rats with an incomplete destruction of the posterior limb of the internal capsule (PLIC) (Pearson's correlation, P<0.05). Alternative therapeutic interventions are required to enhance the potential for recovery in capsular infarct with complete destruction of PLIC.
authors.affiliation: School of Information and Communication, Gwangju Institute of Science and Technology, Gwangju, Republic of Korea.
authors.forename: Donghyeon
authors.initials: D
authors.lastname: Kim
authors.affiliation: Department of Medical System Engineering and School of Mechatronics, Gwangju Institute of Science and Technology, Gwangju, Republic of Korea.
authors.forename: Ra Gyung
authors.initials: RG
authors.lastname: Kim
authors.affiliation: Department of Medical System Engineering and School of Mechatronics, Gwangju Institute of Science and Technology, Gwangju, Republic of Korea.
authors.forename: Hyung-Sun
authors.initials: HS
authors.lastname: Kim
authors.affiliation: Department of Pathology, Chonnam National University Medical School, Gwangju, Republic of Korea.
authors.forename: Jin-Myung
authors.initials: JM
authors.lastname: Kim
authors.affiliation: School of Information and Communication, Gwangju Institute of Science and Technology, Gwangju, Republic of Korea.
authors.forename: Sung Chan
authors.initials: SC
authors.lastname: Jun
authors.affiliation: Department of Medical System Engineering and School of Mechatronics, Gwangju Institute of Science and Technology, Gwangju, Republic of Korea.
authors.forename: Boreom
authors.initials: B
authors.lastname: Lee
authors.affiliation: Section on Functional Imaging Methods, Laboratory of Brain and Cognition, National Institute of Mental Health, National Institutes of Health, Bethesda, Maryland, USA.
authors.forename: Hang Joon
authors.initials: HJ
authors.lastname: Jo
authors.affiliation: Translational Neuroimaging Laboratory, McGill Center for Studies in Aging, Douglas Mental Health University Institute, Montreal, Quebec, Canada.
authors.forename: Pedro R
authors.initials: PR
authors.lastname: Neto
authors.affiliation: Department of Pathology, Chonnam National University Medical School, Gwangju, Republic of Korea.
authors.forename: Min-Cheol
authors.initials: MC
authors.lastname: Lee
authors.affiliation: 1] Department of Medical System Engineering and School of Mechatronics, Gwangju Institute of Science and Technology, Gwangju, Republic of Korea [2] Department of Neurosurgery, Presbyterian Medical Center, Jeonju, Republic of Korea.
authors.forename: Hyoung-Ihl
authors.initials: HI
authors.lastname: Kim
mesh_terms: D000818:Animals; D001522:Behavior, Animal / Q000502:physiology*; D001921:Brain / Q000000981:diagnostic imaging / Q000473:pathology / Q000503:physiopathology; D020520:Brain Infarction / Q000000981:diagnostic imaging / Q000378:metabolism / Q000473:pathology* / Q000503:physiopathology; D003627:Data Interpretation, Statistical; D004195:Disease Models, Animal; D019788:Fluorodeoxyglucose F18; D005947:Glucose / Q000378:metabolism; D007091:Image Processing, Computer-Assisted; D020772:Internal Capsule / Q000000981:diagnostic imaging / Q000378:metabolism / Q000473:pathology* / Q000503:physiopathology; D009043:Motor Activity / Q000502:physiology; D049268:Positron-Emission Tomography; D017207:Rats, Sprague-Dawley; D020127:Recovery of Function / Q000502:physiology*; D013997:Time Factors
chemical_list: D019788:Fluorodeoxyglucose F18; D005947:Glucose