title: Remote focal scanning optical projection tomography with an electrically tunable lens.
abstract: We describe a remote focal scanning technique for optical projection tomography (OPT) implemented with an electrically tunable lens (ETL) that removes the need to scan the specimen or objective lens. Using a 4× objective lens the average spatial resolution is improved by ∼46% and the light collection efficiency by a factor of ∼6.76, thereby enabling increased acquisition speed and reduced light dose. This convenient implementation is particularly appropriate for lower magnifications and larger sample diameters where axial objective scanning would encounter problems with speed and stability.
authors.affiliation: Photonics Group, Department of Physics, Imperial College London, SW7 2AZ, UK.
authors.forename: Lingling
authors.initials: L
authors.lastname: Chen
authors.affiliation: Photonics Group, Department of Physics, Imperial College London, SW7 2AZ, UK.
authors.forename: Sunil
authors.initials: S
authors.lastname: Kumar
authors.affiliation: Institute for Chemical Biology, Department of Chemistry, Imperial College London, SW7 2AZ, UK.
authors.forename: Douglas
authors.initials: D
authors.lastname: Kelly
authors.affiliation: Institute for Chemical Biology, Department of Chemistry, Imperial College London, SW7 2AZ, UK.
authors.forename: Natalie
authors.initials: N
authors.lastname: Andrews
authors.affiliation: Division of Cell and Molecular Biology, Department of Life Sciences, Imperial College London, SW7 2AZ, UK ; Centre for Integrative Systems Biology, Department of Life Sciences, Imperial College London, SW7 2AZ, UK.
authors.forename: Margaret J
authors.initials: MJ
authors.lastname: Dallman
authors.affiliation: Photonics Group, Department of Physics, Imperial College London, SW7 2AZ, UK.
authors.forename: Paul M W
authors.initials: PM
authors.lastname: French
authors.affiliation: Photonics Group, Department of Physics, Imperial College London, SW7 2AZ, UK.
authors.forename: James
authors.initials: J
authors.lastname: McGinty
keywords: (170.6900) Three-dimensional microscopy; (170.6960) Tomography