title: Effects on Hardness and Elastic Modulus for DSS-8 Peptide Treatment on Remineralization of Human Dental Tissues.
abstract: Dental remineralization may be achieved by mediating the interactions between tooth surfaces with free ions and biomimetic peptides. We recently developed octuplet repeats of aspartate-serine-serine (DSS-8) peptide, which occurs in high abundance in naturally occurring proteins that are critical for tooth remineralization. In this paper, we evaluated the possible role of DSS-8 in enamel remineralization. Human enamel specimens were demineralized, exposed briefly to DSS-8 solution, and then exposed to concentrated ionic solutions that favor remineralization. Enamel nano-mechanical behaviors, hardness and elastic modulus, at various stages of treatment were determined by nanoindentation. The phase, microstructure and morphology of the resultant surfaces were characterized using the grazing incidence X-ray diffraction (GIXD), variable pressure scanning electron microscopy (VPSEM), and atomic force microscopy (AFM), respectively. Nanoindentation results show that the DSS-8 remineralization effectively improves the mechanical and elastic properties for demineralized enamel.
authors.affiliation: Department of Materials Science and Engineering, University of California, Los Angeles, Los Angeles, CA 90095, U.S.A.
authors.forename: Chia-Chan
authors.initials: CC
authors.lastname: Hsu
authors.affiliation: Department of Materials Science and Engineering, University of California, Los Angeles, Los Angeles, CA 90095, U.S.A ; Department of Materials Science and Engineering, Feng Chia University, Taichung 407, Taiwan, Republic of China.
authors.forename: Hsiu-Ying
authors.initials: HY
authors.lastname: Chung
authors.affiliation: Department of Bioengineering, University of California, Los Angeles, Los Angeles, CA 90095, U.S.A.
authors.forename: Elizabeth Marie
authors.initials: EM
authors.lastname: Hagerman
authors.affiliation: School of Dentistry, University of California, Los Angeles, CA 90095, U.S.A.
authors.forename: Wenyuan
authors.initials: W
authors.lastname: Shi
authors.affiliation: Department of Materials Science and Engineering, University of California, Los Angeles, Los Angeles, CA 90095, U.S.A.
authors.forename: Jenn-Ming
authors.initials: JM
authors.lastname: Yang
authors.affiliation: Department of Materials Science and Engineering, University of California, Los Angeles, Los Angeles, CA 90095, U.S.A ; Department of Bioengineering, University of California, Los Angeles, Los Angeles, CA 90095, U.S.A.
authors.forename: Ben
authors.initials: B
authors.lastname: Wu
keywords: Enamel; Nanoindentation; Peptide; Remineralization