title: Emergence of 2.1. subgenotype of classical swine fever virus in pig population of India in 2011.
abstract: BACKGROUND
  Limited studies are available on molecular epidemiology of classical swine fever virus (CSFV) in India and are restricted to domestic pigs. These studies show the presence of 1.1. genotype.
  
  
  OBJECTIVE
  The aim of the present study was to subgenotype four CSFV isolates, two each from the outbreaks of CSF in wild (Sus scrofa) and domestic pigs of Mizoram state, India, in 2011.
  
  
  METHODS
  CSFV isolates were subjected to nucleotide sequencing in E2 and NS5B genomic regions. Phylogenetic analysis of the isolates in both genomic regions was carried out with 39 Indian isolates (4 isolates from the present study of Mizoram state and 35 isolates from the other states of India) and 57 reference sequences retrieved from the GenBank database. Two of the 39 isolates from India were collected from wild boar and were subgenotyped as 2.1. Out of 37 isolates from domestic pigs, only two were subgenotyped as 2.1.
  
  
  RESULTS
  The analysis revealed the emergence of 2.1. subgenotype of CSFV in both wild and domestic pigs in India.
  
  
  CONCLUSIONS
  The isolates from domestic pigs of Mizoram state (CSF/MZ/KOL/73 and CSF/MZ/AIZ/115) were grouped in genotype 1 and subgenotype 1.1., thus confirming that the source of CSF outbreaks in domesticated pigs in Mizoram was not from wild pigs. The current study forms an essential step for better understanding of the epidemiology of 2.1 subgroup as well as the movement and spread of the disease in India.
authors.affiliation: a Department of Veterinary Pathology , College of Veterinary Sciences & Animal Husbandry, Central Agricultural University , Selesih , Aizawl , Mizoram , India.
authors.forename: T K
authors.initials: TK
authors.lastname: Rajkhowa
authors.forename: Lalthapui
authors.initials: L
authors.lastname: Hauhnar
authors.forename: Isaac
authors.initials: I
authors.lastname: Lalrohlua
authors.forename: Jagan
authors.initials: J
authors.lastname: Mohanarao G
mesh_terms: D000818:Animals; D006691:Classical Swine Fever / Q000453:epidemiology* / Q000235:genetics / Q000821:virology*; D006692:Classical Swine Fever Virus / Q000235:genetics*; D005838:Genotype; D006023:Glycoproteins / Q000235:genetics / Q000378:metabolism; D007194:India / Q000453:epidemiology; D008969:Molecular Sequence Data; D010802:Phylogeny; D017422:Sequence Analysis, DNA / Q000662:veterinary; D013045:Species Specificity; D013552:Swine; D014764:Viral Proteins / Q000235:genetics* / Q000378:metabolism; D015678:Viral Structural Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D006023:Glycoproteins; D014764:Viral Proteins; D015678:Viral Structural Proteins
keywords: E2 gene; India; NS5B gene; classical swine fever; pig; swine; wild boar