title: Synthetic biology for engineering acetyl coenzyme A metabolism in yeast.
abstract: The yeast Saccharomyces cerevisiae is a widely used cell factory for the production of fuels, chemicals, and pharmaceuticals. The use of this cell factory for cost-efficient production of novel fuels and chemicals requires high yields and low by-product production. Many industrially interesting chemicals are biosynthesized from acetyl coenzyme A (acetyl-CoA), which serves as a central precursor metabolite in yeast. To ensure high yields in production of these chemicals, it is necessary to engineer the central carbon metabolism so that ethanol production is minimized (or eliminated) and acetyl-CoA can be formed from glucose in high yield. Here the perspective of generating yeast platform strains that have such properties is discussed in the context of a major breakthrough with expression of a functional pyruvate dehydrogenase complex in the cytosol.
authors.affiliation: Department of Biology and Biological Engineering, Chalmers University of Technology, Gothenburg, SwedenNovo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Hørsholm, Denmark nielsenj@chalmers.se.
authors.forename: Jens
authors.initials: J
authors.lastname: Nielsen
mesh_terms: D000105:Acetyl Coenzyme A / Q000235:genetics* / Q000378:metabolism*; D002244:Carbon / Q000378:metabolism; D000431:Ethanol / Q000378:metabolism; D060847:Metabolic Engineering / Q000379:methods*; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000235:genetics* / Q000378:metabolism; D058615:Synthetic Biology / Q000379:methods
chemical_list: D000431:Ethanol; D000105:Acetyl Coenzyme A; D002244:Carbon