title: A case of primary JC polyomavirus infection-associated nephropathy.
abstract: A 15-year-old boy with a posterior urethral valve received a deceased donor kidney transplant (KT) in March 2011. Basiliximab induction followed by tacrolimus-based triple medication was used as immunosuppression. Eleven months after KT, the graft function deteriorated and the biopsy demonstrated interstitial nephritis suggestive of acute rejection. BK polyomavirus (BKPyV) surveillance in urine and plasma was negative. The patient received methylprednisolone pulses and anti-thymocyte globulin. Immunohistochemistry was positive for simian virus 40 (SV40) large T-antigen (LTag) in the biopsies, and quantitative polymerase chain reaction for JC polyomavirus (JCPyV) indicated high viral loads in urine and borderline levels in plasma. Immunosuppression was reduced and follow-up biopsies showed tubular atrophy and interstitial fibrosis. Two years after KT, antibody-mediated rejection resulted in graft loss and return to hemodialysis. Retrospective serologic work-up indicated a primary JCPyV infection with seroconversion first for IgM, followed by IgG, but no indication of BKPyV infection. In the SV40 LTag positive biopsies, JCPyV deoxyribonucleic acid (DNA) with archetype noncoding control region was detected, while BKPyV DNA was undetectable. To the best of our knowledge, this is the first reported case of primary JCPyV infection as the cause of PyV-associated nephropathy in KT.
authors.affiliation: Department of Virology, Helsinki University Hospital (HUSLAB) and University of Helsinki, Helsinki, Finland.
authors.forename: I
authors.initials: I
authors.lastname: Lautenschlager
authors.forename: T
authors.initials: T
authors.lastname: Jahnukainen
authors.forename: P
authors.initials: P
authors.lastname: Kardas
authors.forename: J
authors.initials: J
authors.lastname: Lohi
authors.forename: E
authors.initials: E
authors.lastname: Auvinen
authors.forename: L
authors.initials: L
authors.lastname: Mannonen
authors.forename: A
authors.initials: A
authors.lastname: Dumoulin
authors.forename: H H
authors.initials: HH
authors.lastname: Hirsch
authors.forename: H
authors.initials: H
authors.lastname: Jalanko
mesh_terms: D000293:Adolescent; D004279:DNA, Viral / Q000235:genetics; D006084:Graft Rejection / Q000175:diagnosis / Q000209:etiology*; D006801:Humans; D007166:Immunosuppressive Agents / Q000627:therapeutic use; D007577:JC Virus / Q000472:pathogenicity; D007676:Kidney Failure, Chronic / Q000150:complications / Q000601:surgery* / Q000821:virology; D016030:Kidney Transplantation; D008297:Male; D009395:Nephritis, Interstitial / Q000175:diagnosis / Q000821:virology*; D027601:Polyomavirus Infections / Q000150:complications / Q000821:virology*; D011183:Postoperative Complications; D011379:Prognosis; D006435:Renal Dialysis; D014412:Tumor Virus Infections / Q000150:complications / Q000821:virology*; D019562:Viral Load
chemical_list: D004279:DNA, Viral; D007166:Immunosuppressive Agents
keywords: biopsy; clinical research/practice; infection and infectious agents; infectious disease; kidney transplantation/nephrology; rejection: acute; rejection: antibody-mediated (ABMR); viral: BK/JC/polyoma