title: Validation of cytogenetic risk groups according to International Prognostic Scoring Systems by peripheral blood CD34+FISH: results from a German diagnostic study in comparison with an international control group.
abstract: International Prognostic Scoring Systems are used to determine the individual risk profile of myelodysplastic syndrome patients. For the assessment of International Prognostic Scoring Systems, an adequate chromosome banding analysis of the bone marrow is essential. Cytogenetic information is not available for a substantial number of patients (5%-20%) with dry marrow or an insufficient number of metaphase cells. For these patients, a valid risk classification is impossible. In the study presented here, the International Prognostic Scoring Systems were validated based on fluorescence in situ hybridization analyses using extended probe panels applied to cluster of differentiation 34 positive (CD34(+)) peripheral blood cells of 328 MDS patients of our prospective multicenter German diagnostic study and compared to chromosome banding results of 2902 previously published patients with myelodysplastic syndromes. For cytogenetic risk classification by fluorescence in situ hybridization analyses of CD34(+) peripheral blood cells, the groups differed significantly for overall and leukemia-free survival by uni- and multivariate analyses without discrepancies between treated and untreated patients. Including cytogenetic data of fluorescence in situ hybridization analyses of peripheral CD34(+) blood cells (instead of bone marrow banding analysis) into the complete International Prognostic Scoring System assessment, the prognostic risk groups separated significantly for overall and leukemia-free survival. Our data show that a reliable stratification to the risk groups of the International Prognostic Scoring Systems is possible from peripheral blood in patients with missing chromosome banding analysis by using a comprehensive probe panel (clinicaltrials.gov identifier:01355913).
authors.affiliation: Department of Hematology and Medical Oncology, University Medicine of Goettingen, Germany friederike.braulke@med.uni-goettingen.de.
authors.forename: Friederike
authors.initials: F
authors.lastname: Braulke
authors.affiliation: Department of Hematology and Oncology, University of Dresden, Germany.
authors.forename: Uwe
authors.initials: U
authors.lastname: Platzbecker
authors.affiliation: Department of Hematology and Oncology, Technical University of Munich, Germany.
authors.forename: Catharina
authors.initials: C
authors.lastname: Müller-Thomas
authors.affiliation: Department of Hematology and Oncology, Technical University of Munich, Germany.
authors.forename: Katharina
authors.initials: K
authors.lastname: Götze
authors.affiliation: Department of Hematology and Oncology, University of Duesseldorf, Germany.
authors.forename: Ulrich
authors.initials: U
authors.lastname: Germing
authors.affiliation: Department of Hematology and Oncology, Uniklinik, Rheinisch-Westfälische Technische Hochschule (RWTH) Aachen, Germany.
authors.forename: Tim H
authors.initials: TH
authors.lastname: Brümmendorf
authors.affiliation: Department of Hematology and Oncology, University Hospital of Mannheim, Germany.
authors.forename: Florian
authors.initials: F
authors.lastname: Nolte
authors.affiliation: Department of Hematology and Oncology, University Hospital of Mannheim, Germany.
authors.forename: Wolf-Karsten
authors.initials: WK
authors.lastname: Hofmann
authors.affiliation: Department of Hematology and Oncology, Marienhospital, Duesseldorf, Germany.
authors.forename: Aristoteles A N
authors.initials: AA
authors.lastname: Giagounidis
authors.affiliation: Department of Hematology and Oncology, University of Freiburg Medical Center, Germany.
authors.forename: Michael
authors.initials: M
authors.lastname: Lübbert
authors.affiliation: Department of Hematology, Stanford University Cancer Center, CA, USA.
authors.forename: Peter L
authors.initials: PL
authors.lastname: Greenberg
authors.affiliation: University of Rochester Medical Center, NY, USA.
authors.forename: John M
authors.initials: JM
authors.lastname: Bennett
authors.affiliation: Institut de Recerca Contra la Leucemia Josep Carreras, Badalona, Spain.
authors.forename: Francesc
authors.initials: F
authors.lastname: Solé
authors.affiliation: Institut de Recerca Contra la Leucemia Josep Carreras, Badalona, Spain.
authors.forename: Mar
authors.initials: M
authors.lastname: Mallo
authors.affiliation: Sonora Quest Laboratories, Phoenix, AZ, USA.
authors.forename: Marilyn L
authors.initials: ML
authors.lastname: Slovak
authors.affiliation: Tokyo Medical University, Tokyo, Japan.
authors.forename: Kazuma
authors.initials: K
authors.lastname: Ohyashiki
authors.affiliation: University of Chicago, IL, USA.
authors.forename: Michelle M
authors.initials: MM
authors.lastname: Le Beau
authors.affiliation: Hanusch Hospital, Boltzmann Institute for Leukemia Research, Vienna, Austria.
authors.forename: Heinz
authors.initials: H
authors.lastname: Tüchler
authors.affiliation: Third Medical Department for Hematology and Oncology and L. Boltzmann Institute for Leukemia Research and Hematology, Hanusch Hospital, Vienna, Austria.
authors.forename: Michael
authors.initials: M
authors.lastname: Pfeilstöcker
authors.affiliation: Third Medical Department for Hematology and Oncology and L. Boltzmann Institute for Leukemia Research and Hematology, Hanusch Hospital, Vienna, Austria.
authors.forename: Thomas
authors.initials: T
authors.lastname: Nösslinger
authors.affiliation: Department of Human Genetics, University of Düsseldorf, Germany.
authors.forename: Barbara
authors.initials: B
authors.lastname: Hildebrandt
authors.affiliation: Department of Hematology and Medical Oncology, University Medicine of Goettingen, Germany.
authors.forename: Katayoon
authors.initials: K
authors.lastname: Shirneshan
authors.affiliation: Department of Hematology, Oncology, and Clinical Immunology, St. Johannes Hospital, Duisburg, Germany.
authors.forename: Carlo
authors.initials: C
authors.lastname: Aul
authors.affiliation: Department of Internal Medicine, Innsbruck Medical University, Austria.
authors.forename: Reinhard
authors.initials: R
authors.lastname: Stauder
authors.affiliation: Department of Internal Medicine I, Division of Hematology and Hemostaseology, Medical University of Vienna, Austria.
authors.forename: Wolfgang R
authors.initials: WR
authors.lastname: Sperr
authors.affiliation: Department of Internal Medicine I, Division of Hematology and Hemostaseology, Medical University of Vienna, Austria.
authors.forename: Peter
authors.initials: P
authors.lastname: Valent
authors.affiliation: Department of Medical Genetics, Medical University of Vienna, Vienna, Austria.
authors.forename: Christa
authors.initials: C
authors.lastname: Fonatsch
authors.affiliation: Department of Hematology and Medical Oncology, University Medicine of Goettingen, Germany.
authors.forename: Lorenz
authors.initials: L
authors.lastname: Trümper
authors.affiliation: Department of Hematology and Medical Oncology, University Medicine of Goettingen, Germany.
authors.forename: Detlef
authors.initials: D
authors.lastname: Haase
authors.affiliation: Department of Hematology and Medical Oncology, University Medicine of Goettingen, Germany.
authors.forename: Julie
authors.initials: J
authors.lastname: Schanz
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D018952:Antigens, CD34 / Q000097:blood*; D016022:Case-Control Studies; D002869:Chromosome Aberrations; D020732:Cytogenetic Analysis / Q000379:methods*; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D007390:International Agencies; D008297:Male; D008875:Middle Aged; D009190:Myelodysplastic Syndromes / Q000235:genetics* / Q000401:mortality / Q000473:pathology*; D009367:Neoplasm Staging; D011379:Prognosis; D011446:Prospective Studies; D015996:Survival Rate; D055815:Young Adult
chemical_list: D018952:Antigens, CD34