title: Identification of a napin-like peptide from Eugenia malaccensis L. seeds with inhibitory activity toward Staphylococcus aureus and Salmonella Enteritidis.
abstract: This study aimed to purify and characterize peptides from the seeds of Eugenia malaccensis, L. (jambo) with inhibitory activity against the foodborne pathogens Staphylococcus aureus and Salmonella Enteritidis. Crude extract (CE), precipitate fraction 30-60 % and molecules between 3.5 and 10 kDa obtained from precipitate fraction 30-60 % (Em2) showed inhibitory activity against the tested bacterial strains. The highest antibacterial activity was observed for Em2 against S. aureus. The major peak eluted at approximately 30 % in an acetonitrile gradient in reverse-phase chromatography of Em2 (Em2-F1 to Em2-F19), and it showed the highest antibacterial activity, which was twofold higher against S. aureus than against S. Enteritidis. MALDI-ToF spectra of Em2-F18 revealed a molecular mass of 1,231.1 Da and the amino acid sequence showed high identity to the napin family. These findings report for the first time a napin-like peptide from E. malaccensis L. seeds with potential to be applied as a new anti-Staphylococcus molecule.
authors.affiliation: Laboratory of Microbial Processing in Foods, Department of Food Engineering, Center of Technology, Federal University of Paraíba, João Pessoa, Brazil.
authors.forename: Claudenise Caldas
authors.initials: CC
authors.lastname: da Silva Dantas
authors.forename: Evandro Leite
authors.initials: EL
authors.lastname: de Souza
authors.forename: Juscélio Donizete
authors.initials: JD
authors.lastname: Cardoso
authors.forename: Loiane Alves
authors.initials: LA
authors.lastname: de Lima
authors.forename: Kleber
authors.initials: K
authors.lastname: de Sousa Oliveira
authors.forename: Ludovico
authors.initials: L
authors.lastname: Migliolo
authors.forename: Simoni Campos
authors.initials: SC
authors.lastname: Dias
authors.forename: Octávio Luiz
authors.initials: OL
authors.lastname: Franco
authors.forename: Marciane
authors.initials: M
authors.lastname: Magnani
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests; D010940:Plant Proteins / Q000737:chemistry / Q000494:pharmacology*; D012477:Salmonella enteritidis / Q000187:drug effects*; D012639:Seeds / Q000737:chemistry*; D013211:Staphylococcus aureus / Q000187:drug effects*; D027842:Syzygium / Q000737:chemistry*
chemical_list: D000900:Anti-Bacterial Agents; D010940:Plant Proteins