title: In vivo delivery of RCAS virus to mice.
abstract: Injection of RCAS viruses is highly customizable to the desired target tissue. RCAS viruses can be delivered into mice in vivo by injection of virus-producing cells or by injection of concentrated virus. When cells are injected, they persist for several days, continuously producing virus. Typically the decision of whether to inject virus-producing cells or concentrated virus is determined by the volume that can be reliably injected into a given tissue and the age of the animal when the virus delivery is performed. This general protocol describes the intraperitoneal injection of RCAS-expressing cells into mice and discusses the circumstances in which the injection of concentrated virus is preferred.
authors.affiliation: Program in Gene Function and Expression, University of Massachusetts Medical School, Worcester, Massachusetts 01605.
authors.forename: Leanne G
authors.initials: LG
authors.lastname: Ahronian
authors.affiliation: Program in Gene Function and Expression, University of Massachusetts Medical School, Worcester, Massachusetts 01605.
authors.forename: Brian C
authors.initials: BC
authors.lastname: Lewis
mesh_terms: D000818:Animals; D001358:Avian Sarcoma Viruses / Q000235:genetics* / Q000254:growth & development*; D017690:Cell Transplantation / Q000379:methods*; D018014:Gene Transfer Techniques; D005822:Genetic Vectors / Q000008:administration & dosage*; D007274:Injections, Intraperitoneal; D051379:Mice