title: Investigation of male infertility using quantitative comparative proteomics.
abstract: Male factors account for 40% of infertility cases. The identification of differentially expressed proteins on spermatozoa from fertile and infertile men can help in the elucidation of the molecular basis of male infertility. The aim of this study was to compare sperm proteomes from 3 different groups: fertile men, normozoospermic men consulting for infertility, and normozoospermic men with an impaired capacity for fertilization (IVF-failure). We used differential proteomics with isobaric tags for relative and absolute quantitation (iTRAQ) labeling, and LC-MS analysis to identify proteins that are differentially expressed. A total of 348 unique proteins were identified and quantified. The analysis identified 33 proteins that were differentially expressed in the IVF-failure group vs the fertile group. Comparison of the infertile and fertile groups revealed that 18 proteins appeared to be differentially expressed. Four proteins were similarly altered in the IVF-failure and infertile groups: semenogelin 1 (SEMG1), prolactin-induced protein (PIP), glyceraldehyde-3-phosphate dehydrogenase (GAPDHS), and phosphoglycerate kinase 2 (PGK2). These protein markers were selected for validation using multiple reactions monitoring mass spectrometry (MRM-MS) and further confirmed by Western blot analysis. Overall, these results suggest that a panel of proteins may be used as biomarkers for future studies of infertility.
authors.affiliation: Département Obstétrique, Gynécologie et Reproduction and ‡Département Médecine Moléculaire, Centre de Recherche, Centre Hospitalier Universitaire de Québec , Quebec City, Quebec, Canada G1V 4G2.
authors.forename: Christine
authors.initials: C
authors.lastname: Légaré
authors.forename: Arnaud
authors.initials: A
authors.lastname: Droit
authors.forename: Frédéric
authors.initials: F
authors.lastname: Fournier
authors.forename: Sylvie
authors.initials: S
authors.lastname: Bourassa
authors.forename: André
authors.initials: A
authors.lastname: Force
authors.forename: Francine
authors.initials: F
authors.lastname: Cloutier
authors.forename: Roland
authors.initials: R
authors.lastname: Tremblay
authors.forename: Robert
authors.initials: R
authors.lastname: Sullivan
mesh_terms: D015415:Biomarkers / Q000378:metabolism; D015153:Blotting, Western; D002352:Carrier Proteins / Q000378:metabolism; D002853:Chromatography, Liquid; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000378:metabolism; D006023:Glycoproteins / Q000378:metabolism; D006801:Humans; D007248:Infertility, Male / Q000175:diagnosis / Q000378:metabolism*; D007527:Isoenzymes / Q000378:metabolism; D007553:Isotope Labeling / Q000379:methods; D008297:Male; D013058:Mass Spectrometry; D026901:Membrane Transport Proteins; D010735:Phosphoglycerate Kinase / Q000378:metabolism; D060066:Protein Interaction Maps; D020543:Proteome / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*; D029607:Seminal Vesicle Secretory Proteins / Q000378:metabolism; D013094:Spermatozoa / Q000378:metabolism*
chemical_list: D015415:Biomarkers; D002352:Carrier Proteins; D006023:Glycoproteins; D007527:Isoenzymes; D026901:Membrane Transport Proteins; C488892:PIP protein, human; D020543:Proteome; D029607:Seminal Vesicle Secretory Proteins; C424482:seminal vesicle-specific antigen; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases; D010735:Phosphoglycerate Kinase; C104317:phosphoglycerate kinase, testis specific
keywords: ITRAQ; MRM-based; infertility; proteomics; spermatozoa