title: Prevalence and molecular characterization of Cryptosporidium in goats across four provincial level areas in China.
abstract: This study assessed the prevalence, species and subtypes of Cryptosporidium in goats from Guangdong Province, Hubei Province, Shandong Province, and Shanghai City of China. Six hundred and four fecal samples were collected from twelve goat farms, and the overall infection rate was 11.4% (69/604). Goats infected with Cryptosporidium were found in eleven farms across four provincial areas, and the infection rate ranged from 2.9% (1/35) to 25.0% (9/36). Three Cryptosporidium species were identified. Cryptosporidium xiaoi (45/69, 65.2%) was the dominant species, followed by C. parvum (14/69, 20.3%) and C. ubiquitum (10/69, 14.5%). The infection rate of Cryptosporidium spp. was varied with host age and goat kids were more susceptible to be infected than adult goats. Subtyping C. parvum and C. ubiquitum positive samples revealed C. parvum subtype IIdA19G1 and C. ubiquitum subtype XIIa were the most common subtypes. Other C. parvum subtypes were detected as well, such as IIaA14G2R1, IIaA15G1R1, IIaA15G2R1 and IIaA17G2R1. All of these subtypes have also been detected in humans, suggesting goats may be a potential source of zoonotic cryptosporidiosis. This was the first report of C. parvum subtypes IIaA14G2R1, IIaA15G1R1 and IIaA17G2R1 infecting in goats and the first molecular identification of C. parvum and its subtypes in Chinese goats.
authors.affiliation: Key Laboratory of Animal Parasitology of Ministry of Agriculture, Animal-borne Food Safety Research Center of Chinese Academy of Agricultural Sciences, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, China; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, China.
authors.forename: Rongsheng
authors.initials: R
authors.lastname: Mi
authors.affiliation: Key Laboratory of Animal Parasitology of Ministry of Agriculture, Animal-borne Food Safety Research Center of Chinese Academy of Agricultural Sciences, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, China; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, China.
authors.forename: Xiaojuan
authors.initials: X
authors.lastname: Wang
authors.affiliation: Key Laboratory of Animal Parasitology of Ministry of Agriculture, Animal-borne Food Safety Research Center of Chinese Academy of Agricultural Sciences, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, China; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Huang
authors.affiliation: Key Laboratory of Animal Parasitology of Ministry of Agriculture, Animal-borne Food Safety Research Center of Chinese Academy of Agricultural Sciences, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, China; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Zhou
authors.affiliation: Key Laboratory of Animal Parasitology of Ministry of Agriculture, Animal-borne Food Safety Research Center of Chinese Academy of Agricultural Sciences, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, China; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, China.
authors.forename: Yuxuan
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Key Laboratory of Animal Parasitology of Ministry of Agriculture, Animal-borne Food Safety Research Center of Chinese Academy of Agricultural Sciences, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, China; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, China.
authors.forename: Yongjun
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Lvxiang Town Agricultural Technology Extension Station of Jinshan District, Shanghai, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Chen
authors.affiliation: Tengzhou Animal Husbandry and Veterinary Technology Service Center, Tengzhou, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhu
authors.affiliation: Key Laboratory of Animal Parasitology of Ministry of Agriculture, Animal-borne Food Safety Research Center of Chinese Academy of Agricultural Sciences, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, China; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, China.
authors.forename: Zhaoguo
authors.initials: Z
authors.lastname: Chen
mesh_terms: D000818:Animals; D002681:China / Q000453:epidemiology; D003457:Cryptosporidiosis / Q000453:epidemiology* / Q000235:genetics* / Q000469:parasitology; D003458:Cryptosporidium / Q000502:physiology*; D015511:Goat Diseases / Q000453:epidemiology* / Q000235:genetics* / Q000469:parasitology; D006041:Goats / Q000469:parasitology*; D006801:Humans; D010802:Phylogeny; D015995:Prevalence