title: Highly sensitive detection of DNA hybridization on commercialized graphene-coated surface plasmon resonance interfaces.
abstract: Strategies employed to interface biomolecules with nanomaterials have considerably advanced in recent years and found practical applications in many different research fields. The construction of nucleic acid modified interfaces together with the label-free detection of hybridization events has been one of the major research focuses in science and technology. In this paper, we demonstrate the high interest of graphene-on-metal surface plasmon resonance (SPR) interfaces for the detection of DNA hybridization events in the attomolar concentration range. The strategy consists on the noncovalent functionalization of graphene-coated SPR interfaces with gold nanostars carrying single-stranded DNA (ssDNA). Upon hybridization with its complementary DNA, desorption of the nanostructures takes place and thus enables the sensitive detection of the DNA hybridization event. The DNA sensor exhibits a detection limit of ≈500 aM for complementary DNA with a linear dynamic range up to 10(-8) M. This label-free DNA detection platform should spur off new interest toward the use of commercially available graphene-coated SPR interfaces.
authors.affiliation: Institut de Recherche Interdisciplinaire, USR 3078 CNRS, Université Lille 1 , Parc de la Haute Borne, 50 Avenue de Halley, BP 70478, 59658 Villeneuve d'Ascq, France.
authors.forename: Oleksandr
authors.initials: O
authors.lastname: Zagorodko
authors.forename: Jolanda
authors.initials: J
authors.lastname: Spadavecchia
authors.forename: Aritz Yanguas
authors.initials: AY
authors.lastname: Serrano
authors.forename: Iban
authors.initials: I
authors.lastname: Larroulet
authors.forename: Amaia
authors.initials: A
authors.lastname: Pesquera
authors.forename: Amaia
authors.initials: A
authors.lastname: Zurutuza
authors.forename: Rabah
authors.initials: R
authors.lastname: Boukherroub
authors.forename: Sabine
authors.initials: S
authors.lastname: Szunerits
mesh_terms: D004277:DNA, Single-Stranded / Q000032:analysis*; D006108:Graphite / Q000737:chemistry*; D009693:Nucleic Acid Hybridization; D020349:Surface Plasmon Resonance
chemical_list: D004277:DNA, Single-Stranded; D006108:Graphite