title: Immunohistochemical and calcium imaging methods in wholemount rat retina.
abstract: In this paper we describe the tools, reagents, and the practical steps that are needed for: 1) successful preparation of wholemount retinas for immunohistochemistry and, 2) calcium imaging for the study of voltage gated calcium channel (VGCC) mediated calcium signaling in retinal ganglion cells. The calcium imaging method we describe circumvents issues concerning non-specific loading of displaced amacrine cells in the ganglion cell layer.
authors.affiliation: Department of Neurobiology, University of California, Los Angeles.
authors.forename: Allison
authors.initials: A
authors.lastname: Sargoy
authors.affiliation: Department of Neurobiology, University of California, Los Angeles; Veterans Administration Greater Los Angeles Healthcare System; Departments of Physiology & Biophysics and Ophthalmology & Visual Sciences, Dalhousie University.
authors.forename: Steven
authors.initials: S
authors.lastname: Barnes
authors.affiliation: Department of Neurobiology, University of California, Los Angeles; Veterans Administration Greater Los Angeles Healthcare System; Departments of Neurobiology and Medicine, Jules Stein Eye Institute, CURE-Digestive Diseases Research Center, David Geffen School of Medicine, University of California, Los Angeles.
authors.forename: Nicholas C
authors.initials: NC
authors.lastname: Brecha
authors.affiliation: Department of Neurobiology, University of California, Los Angeles; luisperez@mednet.ucla.edu.
authors.forename: Luis
authors.initials: L
authors.lastname: Pérez De Sevilla Müller
mesh_terms: D000818:Animals; D001369:Axons / Q000378:metabolism; D002118:Calcium / Q000032:analysis* / Q000378:metabolism; D015220:Calcium Channels / Q000378:metabolism; D020013:Calcium Signaling; D005260:Female; D007150:Immunohistochemistry / Q000379:methods; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D012160:Retina / Q000737:chemistry* / Q000166:cytology / Q000378:metabolism; D012165:Retinal Ganglion Cells / Q000378:metabolism
chemical_list: D015220:Calcium Channels; D002118:Calcium