title: A novel human interleukin-24 peptide created by computer-guided design contributes to suppression of proliferation in esophageal squamous cell carcinoma Eca-109 cells.
abstract: Based on the three-dimensional modeling structure of human interleukin-24 (hIL-24) and its most likely active position predicted by solvent accessibility and apparent electrostatic properties, a novel hIL-24 peptide M1 was created by computer-guided molecular design. The cytotoxicity and cell selectivity of M1 were examined in three human carcinoma cell lines and one normal human embryo lung fibroblast cell line (HEL). MTT assay showed that M1 induced growth arrest in two IL-20 receptor complex-positive cancer cell lines (the esophageal squamous cell carcinoma cell line Eca-109 and the melanoma cell line A375), and antibodies against IL-24 or IL-20 receptor complexes significantly neutralized the inhibitory activity. Moreover, M1 had almost no cytotoxicity on the lung cancer A549 cell line, which lacks a full complement of the IL-20 receptor complexes, or on HEL cells that express the IL-20 receptor complexes. These findings demonstrate that M1 could act as an excellent candidate for the induction of growth arrest on receptor complex-positive cancer cells. In summary, the M1 peptide may represent a novel anticancer agent for esophageal squamous cell carcinoma therapy due to its cancer cell selectivity and its relatively low cytotoxicity to normal cells.
authors.affiliation: Department of Thoracic Surgery, Affiliated Hospital of the Academy of Military Medical Sciences, Fengtai, Beijing 100071, P.R. China.
authors.forename: Qunfeng
authors.initials: Q
authors.lastname: Ma
authors.affiliation: Department of Thoracic Surgery, Affiliated Hospital of the Academy of Military Medical Sciences, Fengtai, Beijing 100071, P.R. China.
authors.forename: Xuefeng
authors.initials: X
authors.lastname: Deng
authors.affiliation: College of Life Science and Bioengineering, School of Science, Beijing Jiaotong University, Haidian, Beijing 100044, P.R. China.
authors.forename: Bangming
authors.initials: B
authors.lastname: Jin
authors.affiliation: College of Life Science and Bioengineering, School of Science, Beijing Jiaotong University, Haidian, Beijing 100044, P.R. China.
authors.forename: Yao
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: College of Life Science and Bioengineering, School of Science, Beijing Jiaotong University, Haidian, Beijing 100044, P.R. China.
authors.forename: Dan
authors.initials: D
authors.lastname: Luo
authors.affiliation: College of Life Science and Bioengineering, School of Science, Beijing Jiaotong University, Haidian, Beijing 100044, P.R. China.
authors.forename: Heyu
authors.initials: H
authors.lastname: Song
authors.affiliation: College of Life Science and Bioengineering, School of Science, Beijing Jiaotong University, Haidian, Beijing 100044, P.R. China.
authors.forename: Pengkun
authors.initials: P
authors.lastname: Wang
authors.affiliation: College of Life Science and Bioengineering, School of Science, Beijing Jiaotong University, Haidian, Beijing 100044, P.R. China.
authors.forename: Chi
authors.initials: C
authors.lastname: Zhang
authors.affiliation: College of Life Science and Bioengineering, School of Science, Beijing Jiaotong University, Haidian, Beijing 100044, P.R. China.
authors.forename: Xue
authors.initials: X
authors.lastname: Li
authors.affiliation: College of Life Science and Bioengineering, School of Science, Beijing Jiaotong University, Haidian, Beijing 100044, P.R. China.
authors.forename: Yinan
authors.initials: Y
authors.lastname: Shi
authors.affiliation: College of Life Science, Southwest University, Beibei, Chongqing 400715, P.R. China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Cell Engineering Research Center, The Fourth Military Medical University, Xicheng, Xi'an, Shaanxi 710032, P.R. China.
authors.forename: Zhinan
authors.initials: Z
authors.lastname: Chen
authors.affiliation: College of Life Science and Bioengineering, School of Science, Beijing Jiaotong University, Haidian, Beijing 100044, P.R. China.
authors.forename: Ziling
authors.initials: Z
authors.lastname: Wang
authors.affiliation: College of Life Science and Bioengineering, School of Science, Beijing Jiaotong University, Haidian, Beijing 100044, P.R. China.
authors.forename: Hong
authors.initials: H
authors.lastname: Jiang
mesh_terms: D000970:Antineoplastic Agents / Q000138:chemical synthesis / Q000494:pharmacology*; D002294:Carcinoma, Squamous Cell; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D017076:Computer-Aided Design; D015195:Drug Design; D004938:Esophageal Neoplasms; D000077277:Esophageal Squamous Cell Carcinoma; D006801:Humans; D020128:Inhibitory Concentration 50; D007378:Interleukins / Q000138:chemical synthesis / Q000494:pharmacology*; D008958:Models, Molecular; D011487:Protein Conformation; D000099180:Interleukin-24
chemical_list: D000970:Antineoplastic Agents; D007378:Interleukins; D000099180:Interleukin-24