title: Mating decreases plasma levels of TGFβ1 and regulates myosalpinx expression of TGFβ1/TGFBR3 in the rat.
abstract: Mating shuts down a 2-methoxyestradiol (2ME)-dependent, non-genomic activity that is responsible for accelerating egg transport in the rat oviduct. The aims of this work were to investigate the role of TGFβ1 in this 2ME-reduced activity and to determine the effect of mating on the expression and distribution of TGFβ1 and its receptor TGFBR3 in the rat oviduct. We determined the level of TGFβ1 in the plasma and oviductal fluid at 1, 3, or 6 hr during Day 1 of the oestrous cycle in unmated or mated animals. We then examined if 2ME accelerates oviductal egg transport in unmated rats that were previously treated with a neutralizing TGFβ1 antibody. The expression of Tgfb1 and Tgfbr3 mRNA and the level and distribution of TGFBR3 protein in the oviduct were also determined at these time points. Mating decreased TGFβ1 in the plasma, but not in the oviductal fluid, whereas antibody neutralization of circulating TGFβ1 did not prevent the effect of 2ME on egg transport. Mating decreased Tgfb1 and hastened the increase in TGFBR3 abundance in the myosalpinx. These results indicate that mating decreased circulating levels of TGFβ1 without shutting down the non-genomic 2ME response that normally accelerates egg transport. Levels of Tgfb1 transcript and TGFBR3 protein, however, changed in the myosalpinx of mated rats, suggesting a role for mating-associated factors in the autocrine and paracrine effects of TGFβ in the oviduct.
authors.affiliation: Laboratorio de Inmunología de la Reproducción, Facultad de Química y Biología, Universidad de Santiago de Chile, Santiago, Chile; Centro Para el Desarrollo en Nanociencia y Nanotecnología-CEDENNA, Santiago, Chile.
authors.forename: María L
authors.initials: ML
authors.lastname: Oróstica
authors.forename: John
authors.initials: J
authors.lastname: López
authors.forename: Lidia M
authors.initials: LM
authors.lastname: Zuñiga
authors.forename: Daniella
authors.initials: D
authors.lastname: Utz
authors.forename: Patricia
authors.initials: P
authors.lastname: Díaz
authors.forename: Patricia
authors.initials: P
authors.lastname: Reuquen
authors.forename: Alexis
authors.initials: A
authors.lastname: Parada-Bustamante
authors.forename: Hugo
authors.initials: H
authors.lastname: Cardenas
authors.forename: Pedro A
authors.initials: PA
authors.lastname: Orihuela
mesh_terms: D000077584:2-Methoxyestradiol; D000818:Animals; D017931:DNA Primers / Q000235:genetics; D004797:Enzyme-Linked Immunosorbent Assay; D004958:Estradiol / Q000031:analogs & derivatives / Q000378:metabolism / Q000494:pharmacology; D005187:Fallopian Tubes / Q000378:metabolism*; D005260:Female; D005455:Fluorescent Antibody Technique; D015151:Immunoblotting; D009130:Muscle, Smooth / Q000378:metabolism*; D011509:Proteoglycans / Q000378:metabolism*; D051381:Rats; D060888:Real-Time Polymerase Chain Reaction; D018125:Receptors, Transforming Growth Factor beta / Q000378:metabolism*; D012726:Sexual Behavior, Animal / Q000502:physiology*; D018709:Statistics, Nonparametric; D053773:Transforming Growth Factor beta1 / Q000097:blood / Q000378:metabolism*
chemical_list: D017931:DNA Primers; D011509:Proteoglycans; D018125:Receptors, Transforming Growth Factor beta; D053773:Transforming Growth Factor beta1; C071736:betaglycan; D004958:Estradiol; D000077584:2-Methoxyestradiol