title: miR-382 inhibits tumor growth and enhance chemosensitivity in osteosarcoma.
abstract: Dysregulation of miRNAs is involved in osteosarcoma (OS). Here, we demonstrate that miR-382 is decreased in specimens of OS patients with a poor chemoresponse compared to those with a good chemoresponse. In addition, our clinical data show that decreased miR-382 was associated with poor survival in OS patients. Overexpression of miR-382 inhibited cell growth and chemoresistance by targeting KLF12 and HIPK3, respectively. In contrast, inhibition of miR-382 or overexpression of target genes stimulated OS cell growth and chemoresistance both in vitro and in vivo. Taken together, these findings suggest that miR-382 is a tumor suppressor miRNA and induction of miR-382 is a potential strategy to inhibit OS progression.
authors.affiliation: Department of Orthopaedics, The General Hospital of Chinese People's Liberation Army, Beijing, China.
authors.forename: Meng
authors.initials: M
authors.lastname: Xu
authors.affiliation: Department of Pharmaceutical Sciences, College of Pharmacy, University of South Florida, Tampa, FL, USA.
authors.forename: Hua
authors.initials: H
authors.lastname: Jin
authors.affiliation: Departments of Molecular Oncology, H. Lee Moffitt Cancer Center and Research Institute, Tampa, FL, USA.
authors.forename: Cheng-Xiong
authors.initials: CX
authors.lastname: Xu
authors.affiliation: State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Sun
authors.affiliation: Department of Orthopaedics, The General Hospital of Chinese People's Liberation Army, Beijing, China.
authors.forename: Zhi
authors.initials: Z
authors.lastname: Mao
authors.affiliation: Department of Orthopaedics, The General Hospital of Chinese People's Liberation Army, Beijing, China.
authors.forename: Wen-Zhi
authors.initials: WZ
authors.lastname: Bi
authors.affiliation: Department of Orthopaedics, The General Hospital of Chinese People's Liberation Army, Beijing, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000235:genetics; D001859:Bone Neoplasms / Q000235:genetics / Q000401:mortality / Q000473:pathology; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics; D019008:Drug Resistance, Neoplasm / Q000235:genetics*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000096:biosynthesis* / Q000235:genetics; D051741:Kruppel-Like Transcription Factors / Q000096:biosynthesis* / Q000235:genetics; D051379:Mice; D008819:Mice, Nude; D035683:MicroRNAs / Q000037:antagonists & inhibitors / Q000096:biosynthesis / Q000235:genetics*; D012516:Osteosarcoma / Q000188:drug therapy* / Q000235:genetics / Q000401:mortality / Q000473:pathology; D017346:Protein Serine-Threonine Kinases / Q000096:biosynthesis* / Q000235:genetics; D016896:Treatment Outcome
chemical_list: D047908:Intracellular Signaling Peptides and Proteins; C436492:KLF12 protein, human; D051741:Kruppel-Like Transcription Factors; C555413:MIRN382 microRNA, human; D035683:MicroRNAs; C109102:HIPK3 protein, human; D017346:Protein Serine-Threonine Kinases