title: Novel variable number of tandem repeats of gibbon MAOA gene and its evolutionary significance.
abstract: Variable number of tandem repeats (VNTRs) are scattered throughout the primate genome, and genetic variation of these VNTRs have been accumulated during primate radiation. Here, we analyzed VNTRs upstream of the monoamine oxidase A (MAOA) gene in 11 different gibbon species. An abundance of truncated VNTR sequences and copy number differences were observed compared to those of human VNTR sequences. To better understand the biological role of these VNTRs, a luciferase activity assay was conducted and results indicated that selected VNTR sequences of the MAOA gene from human and three different gibbon species (Hylobates klossii, Hylobates lar, and Nomascus concolor) showed silencing ability. Together, these data could be useful for understanding the evolutionary history and functional significance of MAOA VNTR sequences in gibbon species.
authors.affiliation: a Department of Biological Sciences, College of Natural Sciences, Pusan National University, Busan 609-735, Republic of Korea.
authors.forename: Yuri
authors.initials: Y
authors.lastname: Choi
authors.forename: Yi-Deun
authors.initials: YD
authors.lastname: Jung
authors.forename: Selvam
authors.initials: S
authors.lastname: Ayarpadikannan
authors.forename: Akihiko
authors.initials: A
authors.lastname: Koga
authors.forename: Hiroo
authors.initials: H
authors.lastname: Imai
authors.forename: Hirohisa
authors.initials: H
authors.lastname: Hirai
authors.forename: Christian
authors.initials: C
authors.lastname: Roos
authors.forename: Heui-Soo
authors.initials: HS
authors.lastname: Kim
mesh_terms: D000818:Animals; D001483:Base Sequence; D045744:Cell Line, Tumor; D003001:Cloning, Molecular; D019295:Computational Biology; D017931:DNA Primers / Q000235:genetics; D019143:Evolution, Molecular; D006801:Humans; D006922:Hylobates / Q000235:genetics*; D008156:Luciferases; D018598:Minisatellite Repeats / Q000235:genetics*; D008969:Molecular Sequence Data; D008995:Monoamine Oxidase / Q000235:genetics*; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D013045:Species Specificity
chemical_list: D017931:DNA Primers; D008156:Luciferases; D008995:Monoamine Oxidase
keywords: evolution; gibbon; monoamine oxidase A; monoamine oxydase A; répresseur; répétitions en tandem en nombre variable; silencer; variable number of tandem repeats; évolution