title: Microsatellite abundance across the Anthozoa and Hydrozoa in the phylum Cnidaria.
abstract: BACKGROUND
  Microsatellite loci have high mutation rates and thus are indicative of mutational processes within the genome. By concentrating on the symbiotic and aposymbiotic cnidarians, we investigated if microsatellite abundances follow a phylogenetic or ecological pattern. Individuals from eight species were shotgun sequenced using 454 GS-FLX Titanium technology. Sequences from the three available cnidarian genomes (Nematostella vectensis, Hydra magnipapillata and Acropora digitifera) were added to the analysis for a total of eleven species representing two classes, three subclasses and eight orders within the phylum Cnidaria.
  
  
  RESULTS
  Trinucleotide and tetranucleotide repeats were the most abundant motifs, followed by hexa- and dinucleotides. Pentanucleotides were the least abundant motif in the data set. Hierarchical clustering and log likelihood ratio tests revealed a weak relationship between phylogeny and microsatellite content. Further, comparisons between cnidaria harboring intracellular dinoflagellates and those that do not, show microsatellite coverage is higher in the latter group.
  
  
  CONCLUSIONS
  Our results support previous studies that found tri- and tetranucleotides to be the most abundant motifs in invertebrates. Differences in microsatellite coverage and composition between symbiotic and non-symbiotic cnidaria suggest the presence/absence of dinoflagellates might place restrictions on the host genome.
authors.affiliation: Department of Biology, Pennsylvania State University, 208 Mueller Laboratory, University Park, PA 16802, USA. dvr116@psu.edu.
authors.forename: Dannise V
authors.initials: DV
authors.lastname: Ruiz-Ramos
authors.forename: Iliana B
authors.initials: IB
authors.lastname: Baums
mesh_terms: D000818:Animals; D037421:Anthozoa / Q000145:classification / Q000235:genetics* / Q000502:physiology; D019143:Evolution, Molecular; D037402:Hydrozoa / Q000145:classification / Q000235:genetics* / Q000502:physiology; D018895:Microsatellite Repeats; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D013559:Symbiosis