title: KGFR promotes Na+ channel expression in a rat acute lung injury model.
abstract: BACKGROUND
  Binding of keratinocyte growth factor (KGF) to the KGF receptor (KGFR) plays an important role in the recovery of alveolar epithelial cells from acute lung injury (ALI).
  
  
  OBJECTIVE
  To evaluate the effect of gene therapy via adenovirus gene transfer of KGFR on the treatment of ALI.
  
  
  METHODS
  Sprague-Dawley rats were divided into four groups: normal controls, injury controls, normal adenovirus transduced group and injury adenovirus transduced group. The ALI model was induced by lipopolysaccharide (LPS) injection. Recombinant adenovirus (AdEasy-KGFR) was injected via the tail vein. Expression of the sodium (Na(+)) channel in rat alveolar type II (ATII) epithelial cells was determined by PCR, immunohistochemistry and immunoelectron microscopy of rat lung tissues.
  
  
  RESULTS
  Gene expression of the Na(+) channel and KGFR in ATII cells was higher in the normal adenovirus transduced group than the three other groups; expression of these two genes in the injury adenovirus transduced group was higher than the injury control group. Na(+) channel protein expression was lower in the injury adenovirus transduced group but higher than the injury control group.
  
  
  CONCLUSIONS
  KGFR over-expression induced Na channel expression could potentially be beneficial for ALI therapy.
authors.affiliation: Laboratory department, 161st Central Hospital of P.L.A., Wuhan 430010, Hubei Province, China.
authors.forename: Binjian
authors.initials: B
authors.lastname: Liu
authors.affiliation: Department of Microbiology, the Fourth Military Medical University, Xi'an 710032, Shaanxi Province, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Lü
authors.affiliation: Laboratory department, 161st Central Hospital of P.L.A., Wuhan 430010, Hubei Province, China.
authors.forename: Chaoling
authors.initials: C
authors.lastname: Qi
authors.affiliation: Laboratory department, 161st Central Hospital of P.L.A., Wuhan 430010, Hubei Province, China.
authors.forename: Shuhui
authors.initials: S
authors.lastname: Zheng
authors.affiliation: Laboratory department, 161st Central Hospital of P.L.A., Wuhan 430010, Hubei Province, China.
authors.forename: Muxiu
authors.initials: M
authors.lastname: Zhou
authors.affiliation: Institute of Surgery Research, the Third Military Medical University, Chongqing 400042, China.
authors.forename: Jianmin
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Microbiology, the Fourth Military Medical University, Xi'an 710032, Shaanxi Province, China ; Central Laboratory, the Fourth Military Medical University, Xi'an 710032, Shaanxi Province, China.
authors.forename: Wen
authors.initials: W
authors.lastname: Yin
mesh_terms: D055371:Acute Lung Injury / Q000139:chemically induced* / Q000235:genetics / Q000378:metabolism*; D000256:Adenoviridae / Q000235:genetics; D000818:Animals; D004195:Disease Models, Animal; D053503:Epithelial Sodium Channels / Q000235:genetics* / Q000378:metabolism; D051523:Fibroblast Growth Factor 7 / Q000235:genetics*; D015316:Genetic Therapy / Q000379:methods*; D007150:Immunohistochemistry; D008297:Male; D016253:Microscopy, Immunoelectron; D016133:Polymerase Chain Reaction; D011650:Pulmonary Alveoli / Q000166:cytology / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D051497:Receptor, Fibroblast Growth Factor, Type 2 / Q000235:genetics*; D016896:Treatment Outcome
chemical_list: D053503:Epithelial Sodium Channels; C496453:FGF7 protein, human; D051523:Fibroblast Growth Factor 7; D051497:Receptor, Fibroblast Growth Factor, Type 2; C065104:keratinocyte growth factor receptor
keywords: Keratinocyte growth factor-receptor (KGFR); acute lung injury (ALI); gene therapy; rat model; sodium (Na+) channel