title: GABA(A) receptor overexpression in the lateral hypothalamic area attenuates gastric ischemia‑reperfusion injury in rats.
abstract: Excessive activation of the greater splanchnic nerve (GSN) has previously been determined to contribute to the progression of gastric ischemia‑reperfusion (GI‑R) injury. The present study was designed to estimate the protective effects of GABAA receptor (GABA(A)R) overexpression in the lateral hypothalamic area (LHA) against GI‑R injury. The GI‑R injury model was induced in rats by clamping the celiac artery for 30 min and then reperfusing for 1 h. Microinjection of recombinant adenoviral vectors overexpressing GABA(A)R (Ad‑GABA(A)R) or control adenoviral vectors (Ad‑Con) into the LHA was conducted in GI‑R and normal control rats. Significant protective effects were observed on day 2 after Ad‑GABA(A)R treatment in the GI‑R injury rats. Ad‑GABA(A)R treatment reduced plasma norepinephrine levels, plasma angiotensin II levels and peripheral GSN activity, but increased the gastric mucosal blood flow, as compared with Ad‑Con treatment. These results indicate that adenoviral vector‑induced GABA(A)R overexpression in the LHA blunts GSN activity and subsequently alleviates the effects of gastric injury in GI‑R rats.
authors.affiliation: Department of Neurology, The Affiliated Second Hospital of Zhengzhou University, Zhengzhou, Henan 450014, P.R. China.
authors.forename: Lin
authors.initials: L
authors.lastname: Gao
authors.affiliation: Laboratory of Neuropharmacology and Neurotoxicology, Shanghai University, Shanghai 200444, P.R. China.
authors.forename: Tao
authors.initials: T
authors.lastname: Zhu
authors.affiliation: Life Science College of Northeast Agricultural University, Harbin, Heilongjiang 150030, P.R. China.
authors.forename: Guilin
authors.initials: G
authors.lastname: Xie
authors.affiliation: Department of Bioengineering, College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, P.R. China.
authors.forename: Xiangxin
authors.initials: X
authors.lastname: Lou
authors.affiliation: Department of Laboratory Medicine, Lianyungang Hospital Affiliated Bengbu Medical College, Lianyungang, Jiangsu 222006, P.R. China.
authors.forename: Shibao
authors.initials: S
authors.lastname: Li
authors.affiliation: Department of Laboratory Medicine, Lianyungang Hospital Affiliated Bengbu Medical College, Lianyungang, Jiangsu 222006, P.R. China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: Department of Life Science, Heze University, Heze, Shandong 274500, P.R. China.
authors.forename: Zhenxu
authors.initials: Z
authors.lastname: Deng
authors.affiliation: Department of Life Science, Heze University, Heze, Shandong 274500, P.R. China.
authors.forename: Dechang
authors.initials: D
authors.lastname: Chu
authors.affiliation: Department of Neurology, The Affiliated Second Hospital of Zhengzhou University, Zhengzhou, Henan 450014, P.R. China.
authors.forename: Jiyu
authors.initials: J
authors.lastname: Lou
authors.affiliation: Laboratory of Neuropharmacology and Neurotoxicology, Shanghai University, Shanghai 200444, P.R. China.
authors.forename: Dongshu
authors.initials: D
authors.lastname: Du
mesh_terms: D000256:Adenoviridae / Q000235:genetics; D000804:Angiotensin II / Q000097:blood; D000818:Animals; D001783:Blood Flow Velocity; D004195:Disease Models, Animal; D005753:Gastric Mucosa / Q000098:blood supply; D005822:Genetic Vectors / Q000378:metabolism; D007026:Hypothalamic Area, Lateral / Q000378:metabolism*; D007150:Immunohistochemistry; D008297:Male; D009638:Norepinephrine / Q000097:blood; D051381:Rats; D017207:Rats, Sprague-Dawley; D011963:Receptors, GABA-A / Q000235:genetics / Q000378:metabolism*; D015427:Reperfusion Injury / Q000378:metabolism / Q000473:pathology*; D013153:Splanchnic Nerves / Q000378:metabolism / Q000473:pathology; D013270:Stomach / Q000473:pathology
chemical_list: D011963:Receptors, GABA-A; D000804:Angiotensin II; D009638:Norepinephrine