title: Dynamic pathology for circulating free DNA in a dextran sodium sulfate colitis mouse model.
abstract: OBJECTIVE
  In sepsis, circulating free DNA (cf-DNA) is increased, and is a marker of severity and prognosis of septic patients. This study aimed to evaluate cf-DNA in a dextran sodium sulfate-induced colitis mouse model, and its clinical implications.
  
  
  METHODS
  Dynamic pathology of the cecum wall in the DSS-induced colitis mouse model was analyzed using multiphoton microscopy (MPM). Plasma cf-DNA concentrations in colitis mouse were quantified using PicoGreen dsDNA Assay Kit. Plasma cf-DNA was also measured in 123 human ulcerative colitis (UC) patients [mean age: 35.9 years (3-75 years) with 20 pediatric patients] to assess its relationships with clinical severity and Matt's grade.
  
  
  RESULTS
  Real-time images of cf-DNA were detected in the colitis model. The amount of labeled cf-DNA in the circulation of the colitis mice group was significantly higher compared with that in the control group (P < 0.05). In human UC blood samples, plasma cf-DNA concentrations in UC patients were significantly positively correlated with the clinical severity of UC and Matt's grade (P < 0.05, P < 0.05, respectively).
  
  
  CONCLUSIONS
  Using MPM, we observed and analyzed real-time images of cf-DNA in a colitis mouse model. Plasma cf-DNA is a potential non-invasive blood marker for reflecting clinical severity and mucosal damage in UC patients.
authors.affiliation: Department of Gastrointestinal and Pediatric Surgery, Mie University Graduate School of Medicine, 2-174 Edobashi, Tsu, Mie, 514-8507, Japan, koikyon@clin.medic.mie-u.ac.jp.
authors.forename: Yuhki
authors.initials: Y
authors.lastname: Koike
authors.forename: Keiichi
authors.initials: K
authors.lastname: Uchida
authors.forename: Koji
authors.initials: K
authors.lastname: Tanaka
authors.forename: Shozo
authors.initials: S
authors.lastname: Ide
authors.forename: Kohei
authors.initials: K
authors.lastname: Otake
authors.forename: Yoshiki
authors.initials: Y
authors.lastname: Okita
authors.forename: Mikihiro
authors.initials: M
authors.lastname: Inoue
authors.forename: Toshimitsu
authors.initials: T
authors.lastname: Araki
authors.forename: Akira
authors.initials: A
authors.lastname: Mizoguchi
authors.forename: Masato
authors.initials: M
authors.lastname: Kusunoki
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000818:Animals; D015415:Biomarkers / Q000097:blood; D002648:Child; D002675:Child, Preschool; D003092:Colitis / Q000097:blood / Q000139:chemically induced / Q000473:pathology*; D004247:DNA / Q000097:blood*; D016264:Dextran Sulfate / Q000633:toxicity; D005260:Female; D006801:Humans; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008856:Microscopy, Fluorescence; D008875:Middle Aged; D011379:Prognosis; D012720:Severity of Illness Index; D055815:Young Adult
chemical_list: D015415:Biomarkers; D004247:DNA; D016264:Dextran Sulfate