title: Olig3 is not involved in the ventral patterning of spinal cord.
abstract: At embryonic stages, Olig3 is initially expressed in the dorsal-most region of the spinal cord, but later in the ventral marginal zone as well. Previous studies indicated that Olig3 controlled the patterning of dorsal spinal cord and loss of Olig3 function led to the re-specification of dI2 and dI3 neurons into dI4 interneurons. However, the role of Olig3 in regulating the development of ventral spinal cord has remained unknown. BrdU labeling demonstrated that ventral Olig3 was expressed in the post-mitotic neurons and Olig3+ cells seen at late embryonic stages were born at the earlier stage but remained in the marginal zone throughout embryogenesis. Loss-of-function and gain-of-function experiment indicated that Nkx2.2 regulated the expression of Olig3 in V3 interneurons. However, Olig3 mutation didn't apparently affect the generation and migration of ventral neurons. These findings suggest that Olig3 plays different roles in regulating the development of dorsal and ventral spinal cord.
authors.affiliation: Department of Anatomical Sciences and Neurobiology, University of Louisville, Louisville, KY, United States of America; Beijing Institute of Biotechnology, Beijing, PR China.
authors.forename: Zijing
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Department of Anatomical Sciences and Neurobiology, University of Louisville, Louisville, KY, United States of America.
authors.forename: Xuemei
authors.initials: X
authors.lastname: Hu
authors.affiliation: Beijing Institute of Biotechnology, Beijing, PR China.
authors.forename: Chengcheng
authors.initials: C
authors.lastname: Huang
authors.affiliation: Institute of Developmental and Regenerative Biology, Zhejiang Key Laboratory of Organ Development and Regeneration, Hangzhou Normal University, Hangzhou, PR China.
authors.forename: Kang
authors.initials: K
authors.lastname: Zheng
authors.affiliation: Division of Neurobiology and Anatomy, Graduate School of Medical and Dental Sciences, Niigata University, Niigata, Japan.
authors.forename: Hirohide
authors.initials: H
authors.lastname: Takebayashi
authors.affiliation: Beijing Institute of Biotechnology, Beijing, PR China.
authors.forename: Cheng
authors.initials: C
authors.lastname: Cao
authors.affiliation: Department of Anatomical Sciences and Neurobiology, University of Louisville, Louisville, KY, United States of America; Institute of Developmental and Regenerative Biology, Zhejiang Key Laboratory of Organ Development and Regeneration, Hangzhou Normal University, Hangzhou, PR China.
authors.forename: Mengsheng
authors.initials: M
authors.lastname: Qiu
mesh_terms: D000818:Animals; D051792:Basic Helix-Loop-Helix Proteins / Q000235:genetics / Q000502:physiology*; D019521:Body Patterning; D002452:Cell Count; D002454:Cell Differentiation; D002642:Chick Embryo; D018274:Electroporation; D018507:Gene Expression Regulation, Developmental; D005838:Genotype; D000090322:Homeobox Protein Nkx-2.2; D018398:Homeodomain Proteins / Q000378:metabolism; D007395:Interneurons / Q000378:metabolism; D051379:Mice; D008938:Mitosis; D009154:Mutation; D009474:Neurons / Q000378:metabolism; D013116:Spinal Cord / Q000196:embryology* / Q000378:metabolism*; D014157:Transcription Factors / Q000378:metabolism; D029961:Zebrafish Proteins
chemical_list: D051792:Basic Helix-Loop-Helix Proteins; D000090322:Homeobox Protein Nkx-2.2; D018398:Homeodomain Proteins; D014157:Transcription Factors; D029961:Zebrafish Proteins; C000715627:Nkx2-2 protein, mouse; C420992:Olig3 protein, mouse; C000715630:nkx2.2b protein, zebrafish