title: [Effect of bile acids on surface tension of bronchoalveolar lavage fluid in rabbits].
abstract: OBJECTIVE
  To observe changes in surface tension of bronchoalveolar lavage fluids (BALF) in rabbits with hyperbilirubinemia and the influence of bile diluents and 5 different bile acids on BALF surface tension to provide better insight into the regulatory role of bile acids on respiratory function.
  
  
  METHODS
  Bronchoalveolar lavage with 0.9% normal saline was carried out in 30 male New Zealand rabbits and the surface tensions of BALF were measured. The changes in BALF surface tension was measured in rabbits with hyperbilirubinemia. Different concentrations of bile diluents, normal saline, or water solutions of 5 bile acids were added into the collected BALF to test their influence on the surface tension of BALF.
  
  
  RESULTS
  The BALF from rabbits with hyperbilirubinemia showed a significantly increased surface tension (P<0.05). The bile diluents (1:15, 1:10, and 1:5) added into the BALF increased the surface tension of the BALF by 21.15%, 26.09%, and 19.64%, respectively. Among the water solutions of the 5 bile acids, UDCA produced no significant influence on the surface tension of BALF while CDCA, CA, LCA, and DCA increased the surface tension by 16.10%, 21.66%, 14.21%, and 13.05%, respectively.
  
  
  CONCLUSIONS
  The surface tension of BALF increases significantly during hyperbilirubinemia. Bile diluents as well as the free bile acids CDCA, CA, LCA and DCA, but not UDCA, can increase the surface tension of BALF, suggesting that these bile acids may emulsify pulmonary alveolar surfactants to increase the alveolar surface tension.
authors.affiliation: Department of Physiology, Southern Medical University, Guangzhou 510515, China.E-mail: wolf_79@163.com.
authors.forename: Fei
authors.initials: F
authors.lastname: Wang
authors.forename: Cong
authors.initials: C
authors.lastname: Zhao
authors.forename: Yinghong
authors.initials: Y
authors.lastname: Tian
authors.forename: Yanru
authors.initials: Y
authors.lastname: Yin
mesh_terms: D000818:Animals; D001646:Bile; D001647:Bile Acids and Salts; D001992:Bronchoalveolar Lavage Fluid; D008297:Male; D011663:Pulmonary Surfactants; D011817:Rabbits; D013500:Surface Tension
chemical_list: D001647:Bile Acids and Salts; D011663:Pulmonary Surfactants