title: Application of crude and recombinant ELISAs and immunochromatographic test for serodiagnosis of animal trypanosomosis in the Umkhanyakude district of KwaZulu-Natal province, South Africa.
abstract: A total of 231 serum samples were collected from sheep (n=9), goats (n=99) and cattle (n=123) in northeastern KwaZulu-Natal, South Africa. Trypanosome infection was detected using Trypanosoma brucei brucei crude antigen (TbbCA) and T. congolense crude antigen (TcoCA) ELISA assays. Recombinant antigen (T. evansi GM6 which consisted of 4 repeat domains, TeGM6-4r) ELISA and immunochromatographic test (ICT) were also used. Crude antigen ELISA, TeGM6-4r-ELISA and ICT detected 27.3%, 29% and 19.9% of trypanosome seropositive samples, respectively. Trypanosome infection prevalence in cattle and goats was 35.8-46.3% and 0-9.1%, respectively. Out of 9 sheep serum samples, 2-4 sera (22.2-44.4%) were positive. The detection performance of crude and recombinant antigen ELISAs was relatively similar (K=0.6-0.7); both are recommended for reference diagnosis and large scale epidemiological surveys. There is potential application for ICT in on-site diagnosis, but its sensitivity should be improved.
authors.affiliation: OIE Reference Laboratory for Surra, OIE Collaborating Centre, National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, Japan.
authors.forename: Thu-Thuy
authors.initials: TT
authors.lastname: Nguyen
authors.forename: Mono Sophie
authors.initials: MS
authors.lastname: Motsiri
authors.forename: Moeti Oriel
authors.initials: MO
authors.lastname: Taioe
authors.forename: Moses Sibusiso
authors.initials: MS
authors.lastname: Mtshali
authors.forename: Yasuyuki
authors.initials: Y
authors.lastname: Goto
authors.forename: Shin-Ichiro
authors.initials: S
authors.lastname: Kawazu
authors.forename: Oriel Matlhahane Molifi
authors.initials: OM
authors.lastname: Thekisoe
authors.forename: Noboru
authors.initials: N
authors.lastname: Inoue
mesh_terms: D000818:Animals; D000953:Antigens, Protozoan / Q000097:blood; D002417:Cattle; D002418:Cattle Diseases / Q000097:blood / Q000175:diagnosis / Q000453:epidemiology / Q000469:parasitology; D002846:Chromatography, Affinity / Q000662:veterinary*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary*; D015511:Goat Diseases / Q000097:blood / Q000175:diagnosis / Q000453:epidemiology / Q000469:parasitology; D006041:Goats; D012698:Serologic Tests / Q000379:methods / Q000662:veterinary*; D012756:Sheep; D012757:Sheep Diseases / Q000097:blood / Q000175:diagnosis / Q000453:epidemiology / Q000469:parasitology; D013019:South Africa / Q000453:epidemiology; D014345:Trypanosoma / Q000145:classification*; D014353:Trypanosomiasis, African / Q000097:blood / Q000175:diagnosis / Q000453:epidemiology / Q000662:veterinary*
chemical_list: D000953:Antigens, Protozoan