title: Mixed infections with distinct cytomegalovirus glycoprotein B genotypes in Polish pregnant women, fetuses, and newborns.
abstract: The purpose of this investigation was to describe a distribution of cytomegalovirus (CMV) single and multiple genotypes among infected pregnant women, their fetuses, and newborns coming from Central Poland, as well as congenital cytomegaly outcome. The study involved 278 CMV-seropositive pregnant women, of whom 192 were tested for viral DNAemia. Human cytomegalovirus (HCMV) genotyping was performed for 18 of 34 pregnant women carrying the viral DNA and for 12 of their 15 offspring with confirmed HCMV infections. Anti-HCMV antibodies levels were assessed by chemiluminescence immunoassay (CLIA) and enzyme-linked fluorescence assay (ELFA) tests. Viral DNA loads and genotypes were determined by real-time polymerase chain reaction (PCR) assays for the UL55 gene. In the pregnant women, we identified HCMV gB1, gB2, gB3, and gB4 genotypes. Single gB2, gB3, or gB4 genotypes were observed in 14 (77.8 %) women, while multiple gB1-gB2 or gB2-gB3 genotypes were observed in four (22.2 %). Maternal HCMV genotypes determined the genotypes identified in their fetuses and newborns (p ≤ 0.050). Half of them were infected with single HCMV gB1, gB2, or gB3 genotypes and the other half with multiple gB1-gB2 or gB2-gB3 genotypes. Single and multiple genotypes were observed in both asymptomatic and symptomatic congenital cytomegaly, although no gB3 genotype was identified among asymptomatic cases. In Central Poland, infections with single and multiple HCMV strains occur in pregnant women, as well as in their fetuses and neonates, with both asymptomatic and symptomatic infections. HCMV infections identified in mothers seem to be associated with the viral genotypes in their children.
authors.affiliation: Department of Gynecology and Gynecologic Oncology, Polish Mother's Memorial Hospital Research Institute, 281/289 Rzgowska Street, Lodz, 93-338, Poland.
authors.forename: M
authors.initials: M
authors.lastname: Rycel
authors.forename: W
authors.initials: W
authors.lastname: Wujcicka
authors.forename: B
authors.initials: B
authors.lastname: Zawilińska
authors.forename: E
authors.initials: E
authors.lastname: Paradowska
authors.forename: P
authors.initials: P
authors.lastname: Suski
authors.forename: Z
authors.initials: Z
authors.lastname: Gaj
authors.forename: J
authors.initials: J
authors.lastname: Wilczyński
authors.forename: Z
authors.initials: Z
authors.lastname: Leśnikowski
authors.forename: D
authors.initials: D
authors.lastname: Nowakowska
mesh_terms: D000914:Antibodies, Viral / Q000097:blood; D060085:Coinfection / Q000453:epidemiology / Q000821:virology*; D003587:Cytomegalovirus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D003586:Cytomegalovirus Infections / Q000453:epidemiology / Q000821:virology*; D005260:Female; D005333:Fetus; D005838:Genotype; D006801:Humans; D007118:Immunoassay; D007231:Infant, Newborn; D017720:Molecular Epidemiology; D011044:Poland / Q000453:epidemiology; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious / Q000453:epidemiology / Q000821:virology*; D060888:Real-Time Polymerase Chain Reaction; D014759:Viral Envelope Proteins / Q000235:genetics*
chemical_list: D000914:Antibodies, Viral; D014759:Viral Envelope Proteins; C040495:glycoprotein B, Simplexvirus