title: The Evaluation of Dipeptidyl Peptidase (DPP)-IV, α-Glucosidase and Angiotensin Converting Enzyme (ACE) Inhibitory Activities of Whey Proteins Hydrolyzed with Serine Protease Isolated from Asian Pumpkin (Cucurbita ficifolia).
abstract: In the present study, whey protein concentrate (WPC-80) and β-lactoglobulin were hydrolyzed with a noncommercial serine protease isolated from Asian pumpkin (Cucurbita ficifolia). Hydrolysates were further fractionated by ultrafiltration using membranes with cut-offs equal 3 and 10 kDa. Peptide fractions of molecular weight lower than 3 and 3-10 kDa were further subjected to the RP-HPLC. Separated preparations were investigated for their potential as the natural inhibitors of dipeptidyl peptidase (DPP-IV), α-glucosidase and angiotensin converting enzyme (ACE). WPC-80 hydrolysate showed higher inhibitory activities against the three tested enzymes than β-lactoglobulin hydrolysate. Especially high biological activities were exhibited by peptide fractions of molecular weight lower than 3 kDa, with ACE IC50 <0.64 mg/mL and DPP-IV IC50 <0.55 mg/mL. This study suggests that peptides generated from whey proteins may support postprandial glycemia regulation and blood pressure maintenance, and could be used as functional food ingredients in the diet of patients with type 2 diabetes.
authors.affiliation: Department of Animal Products Technology and Quality Management, Wrocław University of Enviromental and Life Sciences, ul. Chełmońskiego 37/41, 51-630 Wrocław, Poland.
authors.forename: Babij
authors.initials: B
authors.lastname: Konrad
authors.affiliation: Department of Animal Products Technology and Quality Management, Wrocław University of Enviromental and Life Sciences, ul. Chełmońskiego 37/41, 51-630 Wrocław, Poland.
authors.forename: Dąbrowska
authors.initials: D
authors.lastname: Anna
authors.affiliation: Department of Animal Products Technology and Quality Management, Wrocław University of Enviromental and Life Sciences, ul. Chełmońskiego 37/41, 51-630 Wrocław, Poland.
authors.forename: Szołtysik
authors.initials: S
authors.lastname: Marek
authors.affiliation: Department of Animal Products Technology and Quality Management, Wrocław University of Enviromental and Life Sciences, ul. Chełmońskiego 37/41, 51-630 Wrocław, Poland.
authors.forename: Pokora
authors.initials: P
authors.lastname: Marta
authors.affiliation: Department of Animal Products Technology and Quality Management, Wrocław University of Enviromental and Life Sciences, ul. Chełmońskiego 37/41, 51-630 Wrocław, Poland.
authors.forename: Zambrowicz
authors.initials: Z
authors.lastname: Aleksandra
authors.affiliation: Department of Animal Products Technology and Quality Management, Wrocław University of Enviromental and Life Sciences, ul. Chełmońskiego 37/41, 51-630 Wrocław, Poland.
authors.forename: Chrzanowska
authors.initials: C
authors.lastname: Józefa
keywords: ACE inhibitors; Asian pumpkin; Bioactive peptides; DPP-IV inhibitors; Serine protease; α-Glucosidase inhibitors