title: MicroRNA-93 suppress colorectal cancer development via Wnt/β-catenin pathway downregulating.
abstract: MicroRNA-93 (miR-93) is involved in several carcinoma progressions. It has been reported that miR-93 acts as a promoter or suppressor in different tumors. However, till now, the role of miR-93 in colon cancer is unclear. Herein, we have found that expression of miR-93 was lower in human colon cancer tissue and colorectal carcinoma cell lines compared with normal colon mucosa. Forced expression of miR-93 in colon cancer cells inhibits colon cancer invasion, migration, and proliferation. Furthermore, miR-93 may downregulate the Wnt/β-catenin pathway, which was confirmed by measuring the expression level of the β-catenin, axin, c-Myc, and cyclin-D1 in this pathway. Mothers against decapentaplegic homolog 7 (Smad7), as an essential molecular protein for nuclear accumulation of β-catenin in the canonical Wnt signaling pathway, is predicted as a putative target gene of miR-93 by the silico method and demonstrated that it may be suppressed by targeting its 3'UTR. These findings showed that miR-93 suppresses colorectal cancer development via downregulating Wnt/β-catenin, at least in part, by targeting Smad7. This study revealed that miR-93 is an important negative regulator in colon cancer and suggested that miR-93 may serve as a novel therapeutic agent that offers benefits for colon cancer treatment.
authors.affiliation: Department of Colorectal Cancer Surgery, Cancer Center, the Second Affiliated Hospital, Harbin Medical University, Harbin, 150086, China.
authors.forename: Qingchao
authors.initials: Q
authors.lastname: Tang
authors.forename: Zhaoxia
authors.initials: Z
authors.lastname: Zou
authors.forename: Chendan
authors.initials: C
authors.lastname: Zou
authors.forename: Qian
authors.initials: Q
authors.lastname: Zhang
authors.forename: Rui
authors.initials: R
authors.lastname: Huang
authors.forename: Xu
authors.initials: X
authors.lastname: Guan
authors.forename: Qiang
authors.initials: Q
authors.lastname: Li
authors.forename: Zhongjing
authors.initials: Z
authors.lastname: Han
authors.forename: Dayong
authors.initials: D
authors.lastname: Wang
authors.forename: Huiyan
authors.initials: H
authors.lastname: Wei
authors.forename: Xu
authors.initials: X
authors.lastname: Gao
authors.forename: Xishan
authors.initials: X
authors.lastname: Wang
mesh_terms: D020413:3' Untranslated Regions; D000818:Animals; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics; D003110:Colonic Neoplasms / Q000235:genetics*; D019938:Cyclin D1 / Q000235:genetics; D015536:Down-Regulation; D015972:Gene Expression Regulation, Neoplastic; D016147:Genes, Tumor Suppressor; D045325:HCT116 Cells; D019073:HT29 Cells; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D035683:MicroRNAs / Q000235:genetics*; D015398:Signal Transduction; D051153:Wnt Proteins / Q000235:genetics*; D051176:beta Catenin / Q000235:genetics
chemical_list: D020413:3' Untranslated Regions; C543350:MIRN93 microRNA, human; D035683:MicroRNAs; D051153:Wnt Proteins; D051176:beta Catenin; D019938:Cyclin D1