title: Genetic diversity and relationships among Chinese Eucommia ulmoides cultivars revealed by sequence-related amplified polymorphism, amplified fragment length polymorphism, and inter-simple sequence repeat markers.
abstract: Sequence-related amplified polymorphism (SRAP), amplified fragment length polymorphism (AFLP), and inter-simple sequence repeat (ISSR) markers were used to estimate the genetic diversity and relationships among Eucommia ulmoides cultivars in China. A total of 240, 192, and 150 DNA fragments were detected by 10 SRAP primer combinations, 10 AFLP primer combinations, and 10 ISSR primers, among which 89.2, 65.1, and 88.0% of the fragments were polymorphic, respectively. Cluster analysis revealed that Qinzhong No. 3, Xiaoyeci, Qinzhong No. 1, and Qinzhong No. 2 formed independent clusters. The other 15 cultivars exhibited two clusters. The results of this study will help in the selection of parents for both genome mapping and crossbreeding purposes.
authors.affiliation: College of Forestry, Northwest A&F University, Yangling, Shaanxi, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Li
authors.affiliation: College of Forestry, Northwest A&F University, Yangling, Shaanxi, China.
authors.forename: S H
authors.initials: SH
authors.lastname: Wang
authors.affiliation: College of Forestry, Northwest A&F University, Yangling, Shaanxi, China lizhouqi@nwsuaf.edu.cn.
authors.forename: Z Q
authors.initials: ZQ
authors.lastname: Li
authors.affiliation: College of Forestry, Northwest A&F University, Yangling, Shaanxi, China.
authors.forename: C F
authors.initials: CF
authors.lastname: Jin
authors.affiliation: College of Forestry, Northwest A&F University, Yangling, Shaanxi, China.
authors.forename: M H
authors.initials: MH
authors.lastname: Liu
mesh_terms: D056915:DNA Copy Number Variations; D031283:Eucommiaceae / Q000235:genetics*; D018895:Microsatellite Repeats; D012150:Polymorphism, Restriction Fragment Length