title: Establishment of cell lines from both myeloma bone marrow and plasmacytoma: SNU_MM1393_BM and SNU_MM1393_SC from a single patient.
abstract: OBJECTIVE
  We tried to establish clinically relevant human myeloma cell lines that can contribute to the understanding of multiple myeloma (MM).
  
  
  METHODS
  Mononuclear cells obtained from MM patient's bone marrow were injected via tail vein in an NRG/SCID mouse. Fourteen weeks after the injection, tumor developed at subcutis of the mouse. The engraftment of MM cells into mouse bone marrow (BM) was also observed. We separated and cultured cells from subcutis and BM.
  
  
  RESULTS
  After the separation and culture of cells from subcutis and BM, we established two cell lines originating from a single patient (SNU_MM1393_BM and SNU_MM1393_SC). Karyotype of the two newly established MM cell lines showed tetraploidy which is different from the karyotype of the patient (diploidy) indicating clonal evolution. In contrast to SNU_MM1393_BM, cell proliferation of SNU_MM1393_SC was IL-6 independent. SNU_MM1393_BM and SNU_MM1393_SC showed high degree of resistance against bortezomib compared to U266 cell line. SNU_MM1393_BM had the greater lethality compared to SNU_MM1393_SC.
  
  
  CONCLUSIONS
  Two cell lines harboring different site tropisms established from a single patient showed differences in cytokine response and lethality. Our newly established cell lines could be used as a tool to understand the biology of multiple myeloma.
authors.affiliation: Department of Internal Medicine, Seoul National University Hospital, 101 Daehak-ro, Jongno-Ku, Seoul 110-744, Republic of Korea.
authors.forename: Youngil
authors.identifier: 0000-0002-4750-931X
authors.initials: Y
authors.lastname: Koh
authors.affiliation: Cancer Research Institute, Seoul National University College of Medicine, 103 Daehak-ro, Jongno-Ku, Seoul 110-799, Republic of Korea.
authors.forename: Woo-June
authors.initials: WJ
authors.lastname: Jung
authors.affiliation: Cancer Research Institute, Seoul National University College of Medicine, 103 Daehak-ro, Jongno-Ku, Seoul 110-799, Republic of Korea ; Functional Genome Institute, PDXen Biosystem Inc., 437 Dongil-ro, Kwangjin-Ku, Seoul 143-901, Republic of Korea.
authors.forename: Kwang-Sung
authors.initials: KS
authors.lastname: Ahn
authors.affiliation: Department of Internal Medicine, Seoul National University Hospital, 101 Daehak-ro, Jongno-Ku, Seoul 110-744, Republic of Korea ; Cancer Research Institute, Seoul National University College of Medicine, 103 Daehak-ro, Jongno-Ku, Seoul 110-799, Republic of Korea.
authors.forename: Sung-Soo
authors.initials: SS
authors.lastname: Yoon
mesh_terms: D000818:Animals; D001853:Bone Marrow / Q000473:pathology*; D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation; D002460:Cell Line; D006801:Humans; D008297:Male; D051379:Mice; D008875:Middle Aged; D009101:Multiple Myeloma / Q000473:pathology*; D010954:Plasmacytoma / Q000473:pathology*