title: Bioorthogonal cleavage and exchange of major histocompatibility complex ligands by employing azobenzene-containing peptides.
abstract: Bioorthogonal cleavable linkers are attractive building blocks for compounds that can be manipulated to study biological and cellular processes. Sodium dithionite sensitive azobenzene-containing (Abc) peptides were applied for the temporary stabilization of recombinant MHC complexes, which can then be employed to generate libraries of MHC tetramers after exchange with a novel epitope. This technology represents an important tool for high-throughput studies of disease-specific T cell responses.
authors.affiliation: Department of Microbiology and Immunology Programme, National University of Singapore (NUS), Life Sciences Institute, #03-05, 28 Medical Drive, 117456 (Singapore).
authors.forename: Joanna A L
authors.initials: JA
authors.lastname: Choo
authors.forename: Sock Yue
authors.initials: SY
authors.lastname: Thong
authors.forename: Jiawei
authors.initials: J
authors.lastname: Yap
authors.forename: Wim J E
authors.initials: WJ
authors.lastname: van Esch
authors.forename: Manfred
authors.initials: M
authors.lastname: Raida
authors.forename: Rob
authors.initials: R
authors.lastname: Meijers
authors.forename: Julien
authors.initials: J
authors.lastname: Lescar
authors.forename: Steven H L
authors.initials: SH
authors.lastname: Verhelst
authors.forename: Gijsbert M
authors.initials: GM
authors.lastname: Grotenbreg
mesh_terms: D000595:Amino Acid Sequence; D001391:Azo Compounds / Q000737:chemistry* / Q000276:immunology; D004227:Dithionite / Q000737:chemistry; D000939:Epitopes / Q000737:chemistry / Q000276:immunology; D015234:HLA-A Antigens / Q000737:chemistry* / Q000276:immunology; D006801:Humans; D008024:Ligands; D008958:Models, Molecular; D010455:Peptides / Q000737:chemistry* / Q000276:immunology; D011994:Recombinant Proteins / Q000737:chemistry / Q000276:immunology
chemical_list: D001391:Azo Compounds; D000939:Epitopes; D015234:HLA-A Antigens; D008024:Ligands; D010455:Peptides; D011994:Recombinant Proteins; D004227:Dithionite; C009850:azobenzene
keywords: bioorthogonal chemistry; cleavable linkers; immunology; major histocompatibility complex; peptidomimetics