title: Genetic Manipulation of Acinetobacter baumannii.
abstract: Acinetobacter baumannii is a Gram-negative nosocomial pathogen of clinical importance. A lack of genetic tools has hindered the research of this organism in the past; however, recently, various methods have been designed, modified, and optimized to facilitate the genetic manipulation of A. baumannii. This unit describes some of the recent genetic advances and new recombinant tools developed for this pathogen, including standard transformation and conjugation techniques specifically developed for the bacteria. As the need to understand the basic biology of A. baumannii increases with the prospect of developing new therapeutics, the use of the basic genetic methods herein can provide the critical first step to identify genes required for infection.
authors.affiliation: Walter Reed Army Institute of Research, Department of Wound Infections, Silver Spring, Maryland.
authors.forename: Anna C
authors.initials: AC
authors.lastname: Jacobs
authors.forename: Mitchell G
authors.initials: MG
authors.lastname: Thompson
authors.forename: Michael
authors.initials: M
authors.lastname: Gebhardt
authors.forename: Brendan W
authors.initials: BW
authors.lastname: Corey
authors.forename: Suleyman
authors.initials: S
authors.lastname: Yildirim
authors.forename: Howard A
authors.initials: HA
authors.lastname: Shuman
authors.forename: Daniel V
authors.initials: DV
authors.lastname: Zurawski
mesh_terms: D040981:Acinetobacter baumannii / Q000235:genetics* / Q000502:physiology; D001431:Bacteriological Techniques; D003227:Conjugation, Genetic; D004269:DNA, Bacterial / Q000235:genetics*; D018274:Electroporation; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology; D018014:Gene Transfer Techniques
chemical_list: D004269:DNA, Bacterial
keywords: Acinetobacter baumannii; conjugation; electroporation; mutagenesis; plasmid; transformation