title: Freeze-dried rat bone marrow mesenchymal stem cell paracrine factors: a simplified novel material for skin wound therapy.
abstract: The mesenchymal stem cell (MSC) supernatant is well known as a rich source of autologous cytokines and universally used for tissue regeneration in current clinical medicine. However, the limitation of conditioned medium used in open-wound repair compels the need to find a more sophisticated way to take advantage of the trophic factors of MSCs. We have now fabricated a three-dimensional membrane from freeze-dried bone marrow mesenchymal stem cells-conditioned medium (FBMSC-CM) using a simple freeze-dried protocol. Scanning electron microscopy images showed the microstructure of the FBMSC-CM membrane (FBMSC-CMM) resembling a mesh containing growth factors. ELISA was used to test the paracrine factors retained in the FBMSC-CMM, and the results indicated that FBMSC-CMM withheld over 80% of the paracrine factors. Live/dead assays were adopted to test the toxicity of the FBMSC-CMM on cultured rat dermal fibroblasts, and the results confirmed its biological safety with low toxicity. Moreover, the FBMSC-CMM could significantly accelerate wound healing and enhance the neovascularization as well as epithelialization through strengthening the trophic factors in the wound bed as determined by immunohistochemical staining. Thus, the ability to maintain paracrine factors and enhance the effectiveness of these growth factors in the wound as well as the simple procedure and economical materials required for production qualifies the FBMSC-CMM to be a candidate biomaterial for open-wound regeneration.
authors.affiliation: 1 Department of Plastic Surgery, Guangzhou General Hospital of Guangzhou Command , The Key Laboratory of Trauma Treatment & Tissue Repair of Tropical Area, PLA, Guangzhou, P.R. China .
authors.forename: Yan
authors.initials: Y
authors.lastname: Peng
authors.forename: Min
authors.initials: M
authors.lastname: Xuan
authors.forename: Jiping
authors.initials: J
authors.lastname: Zou
authors.forename: Hongwei
authors.initials: H
authors.lastname: Liu
authors.forename: Ziyuan
authors.initials: Z
authors.lastname: Zhuo
authors.forename: Yu
authors.initials: Y
authors.lastname: Wan
authors.forename: Biao
authors.initials: B
authors.lastname: Cheng
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000494:pharmacology*; D040761:Biomimetic Materials / Q000494:pharmacology; D001854:Bone Marrow Cells / Q000166:cytology*; D018925:Chemokines / Q000378:metabolism; D017077:Culture Media, Conditioned / Q000494:pharmacology; D020405:Dermis / Q000187:drug effects / Q000473:pathology; D004848:Epithelium / Q000187:drug effects / Q000473:pathology; D005347:Fibroblasts / Q000187:drug effects / Q000473:pathology; D005612:Freeze Drying; D036341:Intercellular Signaling Peptides and Proteins / Q000378:metabolism; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D018919:Neovascularization, Physiologic / Q000187:drug effects; D019899:Paracrine Communication / Q000187:drug effects*; D017207:Rats, Sprague-Dawley; D012867:Skin / Q000098:blood supply / Q000187:drug effects / Q000473:pathology*; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D001672:Biocompatible Materials; D018925:Chemokines; D017077:Culture Media, Conditioned; D036341:Intercellular Signaling Peptides and Proteins