title: An efficient reagent for covalent introduction of alkynes into proteins.
abstract: A cheap and bench-stable reagent was synthesized for direct and covalent introduction of alkynes into tyrosine of target proteins, which can be further modified based on click reaction to achieve fluorescence labelling or PEGylation. This reagent should be a generally useful toolbox for chemical biology and biomaterials.
authors.affiliation: State Key Laboratory of Organic-Inorganic Composites, Beijing University of Chemical Technology, 15 Beisanhuan East Road, Chaoyang District, Beijing 100029, China. yilong@mail.buct.edu.cn.
authors.forename: Jie
authors.initials: J
authors.lastname: Zhang
authors.forename: Dejun
authors.initials: D
authors.lastname: Ma
authors.forename: Dawei
authors.initials: D
authors.lastname: Du
authors.forename: Zhen
authors.initials: Z
authors.lastname: Xi
authors.forename: Long
authors.initials: L
authors.lastname: Yi
mesh_terms: D000480:Alkynes / Q000737:chemistry*; D000818:Animals; D001386:Azides / Q000737:chemistry; D036022:Capsid Proteins / Q000032:analysis; D002417:Cattle; D057930:Click Chemistry; D005456:Fluorescent Dyes / Q000737:chemistry*; D007202:Indicators and Reagents / Q000737:chemistry; D008958:Models, Molecular; D011092:Polyethylene Glycols / Q000737:chemistry; D011506:Proteins / Q000032:analysis*; D012710:Serum Albumin, Bovine / Q000032:analysis; D014027:Tobacco Mosaic Virus / Q000737:chemistry / Q000648:ultrastructure; D014443:Tyrosine / Q000737:chemistry*
chemical_list: D000480:Alkynes; D001386:Azides; D036022:Capsid Proteins; D005456:Fluorescent Dyes; D007202:Indicators and Reagents; D011506:Proteins; D012710:Serum Albumin, Bovine; D011092:Polyethylene Glycols; D014443:Tyrosine