title: Differential proteomic profile of spermatogenic and Sertoli cells from peri-pubertal testes of three different bovine breeds.
abstract: Sub-fertility is one of the most common problems observed in crossbred males, but the etiology remains unknown in most of the cases. Although proteomic differences in the spermatozoa and seminal plasma between breeds have been investigated, the possible differences at the sperm precursor cells and supporting/nourishing cells have not been studied. The present study reports the differential proteomic profile of spermatogenic and Sertoli cells in crossbred and purebred bulls. Testis was removed by unilateral castration of 12 peri-pubertal bulls (10 months age), four each from crossbred (Holstein Friesian × Tharparkar), exotic purebred [Holstein Friesian (HF)] and indigenous purebred [Tharparkar (TP)] bulls. Spermatogenic and Sertoli cells were isolated and subjected to proteomic analysis. Protein extracts from the Sertoli and spermatogenic cells of each breed were analyzed with 2-dimensional difference gel electrophoresis (2D-DIGE) and analyzed with Decyder™ software. Compared to HF, 26 protein spots were over expressed and 14 protein spots were under expressed in spermatogenic cells of crossbred bulls. Similarly, 7 protein spots were over expressed and 15 protein spots were under expressed in the spermatogenic cells of TP bulls compared to that of crossbred bulls. Out of 12 selected protein spots identified through mass spectrometry, Phosphatidyl ethanolamine binding protein was found to be over expressed in the spermatogenic cells of crossbred bulls compared to TP bulls. The protein, gamma actin was found to be over expressed in the Sertoli cells of HF bulls, whereas Speedy Protein-A was found to be over expressed in Sertoli cells of crossbred bulls. It may be concluded that certain proteomic level differences exist in sperm precursor cells and nourishing cells between breeds, which might be associated with differences in the fertility among these breeds.
authors.affiliation: Theriogenology Lab, Livestock Production Management, National Dairy Research Institute Karnal, India.
authors.forename: Utkarsh K
authors.initials: UK
authors.lastname: Tripathi
authors.affiliation: Theriogenology Lab, Livestock Production Management, National Dairy Research Institute Karnal, India.
authors.forename: Muhammad K M
authors.initials: MK
authors.lastname: Aslam
authors.affiliation: Department of Biophysics, All India Institute of Medical Sciences New Delhi, India.
authors.forename: Shashank
authors.initials: S
authors.lastname: Pandey
authors.affiliation: Theriogenology Lab, Livestock Production Management, National Dairy Research Institute Karnal, India.
authors.forename: Samiksha
authors.initials: S
authors.lastname: Nayak
authors.affiliation: Theriogenology Lab, Livestock Production Management, National Dairy Research Institute Karnal, India.
authors.forename: Shivani
authors.initials: S
authors.lastname: Chhillar
authors.affiliation: Department of Biophysics, All India Institute of Medical Sciences New Delhi, India.
authors.forename: A
authors.initials: A
authors.lastname: Srinivasan
authors.affiliation: Theriogenology Lab, Livestock Production Management, National Dairy Research Institute Karnal, India.
authors.forename: T K
authors.initials: TK
authors.lastname: Mohanty
authors.affiliation: Embryo Biotechnology Lab, Animal Biotechnology Centre, National Dairy Research Institute Karnal, India.
authors.forename: Prashant H
authors.initials: PH
authors.lastname: Kadam
authors.affiliation: Embryo Biotechnology Lab, Animal Biotechnology Centre, National Dairy Research Institute Karnal, India.
authors.forename: M S
authors.initials: MS
authors.lastname: Chauhan
authors.affiliation: Department of Biophysics, All India Institute of Medical Sciences New Delhi, India.
authors.forename: Savita
authors.initials: S
authors.lastname: Yadav
authors.affiliation: Theriogenology Lab, Livestock Production Management, National Dairy Research Institute Karnal, India.
authors.forename: Arumugam
authors.initials: A
authors.lastname: Kumaresan
keywords: Sertoli cells; bulls; crossbred; proteomics; purebred; spermatogenic cells