title: The effects of Wubeizi ointment on the proliferation of keloid-derived fibroblasts.
abstract: To evaluate the effectiveness of the Wubeizi (WBZ) ointment on keloid-derived fibroblasts. The primary cells of the keloid-derived fibroblasts were cultured and the effectiveness of the WBZ ointment at different concentrations was examined by MTT colorimetric methods on keloid-derived fibroblasts. The WBZ ointment showed inhibitory effects on proliferating the keloid-derived fibroblasts (P < 0.01)in a time- and dose-dependent manner. The proportion of cells in S stage was significantly higher in each of the WBZ ointment group than in the control group (P<0.01), and the proportion of G2 + M stage cells was significantly lower than that of control group, which was statistically significant (P < 0.01).The inhibitory effects of the S and G2 + M stage increased with higher drug concentrations (P < 0.05).
  
  
  CONCLUSIONS
  The WBZ ointment can inhibit the proliferation of the keloid-derived fibroblasts in a time- and dose- dependent manner.
authors.affiliation: Department of Plastic Surgery, Xuzhou Central Hospital, 199# South Jiefang Road, Xuzhou, 221009, Jiangsu, China, djicun@126.com.
authors.forename: Ji-cun
authors.initials: JC
authors.lastname: Ding
authors.forename: Zhi-ming
authors.initials: ZM
authors.lastname: Tang
authors.forename: Xiao-xiang
authors.initials: XX
authors.lastname: Zhai
authors.forename: Xiang-hui
authors.initials: XH
authors.lastname: Chen
authors.forename: Jing-guo
authors.initials: JG
authors.lastname: Li
authors.forename: Cui-xia
authors.initials: CX
authors.lastname: Zhang
mesh_terms: D002453:Cell Cycle / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D004365:Drugs, Chinese Herbal / Q000494:pharmacology* / Q000627:therapeutic use; D005347:Fibroblasts / Q000187:drug effects* / Q000473:pathology*; D006801:Humans; D007627:Keloid / Q000188:drug therapy / Q000473:pathology*; D009824:Ointments; D029045:Rhus / Q000737:chemistry
chemical_list: D004365:Drugs, Chinese Herbal; D009824:Ointments; C000601190:wubeizi