title: Antimicrobial activity of platelet-rich plasma and other plasma preparations against periodontal pathogens.
abstract: BACKGROUND
  In addition to releasing a pool of growth factors during activation, platelets have many features that indicate their role in the anti-infective host defense. The antimicrobial activities of platelet-rich plasma (PRP) and related plasma preparations against periodontal disease-associated bacteria were evaluated.
  
  
  METHODS
  Four distinct plasma fractions were extracted in the formulation used commonly in dentistry and were tested for their antibacterial properties against three periodontal bacteria: Porphyromonas gingivalis, Aggregatibacter actinomycetemcomitans, and Fusobacterium nucleatum. The minimum inhibitory concentration of each plasma preparation was determined, and in vitro time-kill assays were used to detect their abilities to inhibit bacterial growth. Bacterial adhesion interference and the susceptibility of bacterial adherence by these plasma preparations were also conducted.
  
  
  RESULTS
  All plasma preparations can inhibit bacterial growth, with PRP showing the superior activity. Bacterial growth inhibition by PRP occurred in the first 24 hours after application in the time-kill assay. PRP interfered with P. gingivalis and A. actinomycetemcomitans attachment and enhanced exfoliation of attached P. gingivalis but had no influences on F. nucleatum bacterial adherence.
  
  
  CONCLUSIONS
  PRP expressed antibacterial properties, which may be attributed to platelets possessing additional antimicrobial molecules. The application of PRP on periodontal surgical sites is advisable because of its regenerative potential and its antibacterial effects.
authors.affiliation: School of Dentistry, College of Oral Medicine, Chung Shan Medical University, Taichung, Taiwan.
authors.forename: Li-Chiu
authors.initials: LC
authors.lastname: Yang
authors.forename: Suh-Woan
authors.initials: SW
authors.lastname: Hu
authors.forename: Min
authors.initials: M
authors.lastname: Yan
authors.forename: Jaw-Ji
authors.initials: JJ
authors.lastname: Yang
authors.forename: Sing-Hua
authors.initials: SH
authors.lastname: Tsou
authors.forename: Yuh-Yih
authors.initials: YY
authors.lastname: Lin
mesh_terms: D016976:Aggregatibacter actinomycetemcomitans / Q000187:drug effects*; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001422:Bacterial Adhesion / Q000187:drug effects; D058491:Bacterial Load / Q000187:drug effects; D001431:Bacteriological Techniques; D005337:Fibrin / Q000494:pharmacology; D016967:Fusobacterium nucleatum / Q000187:drug effects*; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D008826:Microbial Sensitivity Tests; D050296:Microbial Viability / Q000187:drug effects; D008875:Middle Aged; D010510:Periodontal Diseases / Q000382:microbiology; D010949:Plasma / Q000502:physiology*; D010976:Platelet Count; D053657:Platelet-Rich Plasma / Q000502:physiology*; D016966:Porphyromonas gingivalis / Q000187:drug effects*; D013997:Time Factors
chemical_list: D000900:Anti-Bacterial Agents; D005337:Fibrin
keywords: Anti-bacterial agents; microbial sensitivity tests; oral surgical procedures; periodontal diseases; platelet-rich plasma