title: The Effect of Layer-by-Layer Assembly Coating on the Proliferation and Differentiation of Neural Stem Cells.
abstract: Nanocoating of a single-cell with biocompatible materials creates a defined microenvironment for cell differentiation and proliferation, as well as a model for studies in cell biology. In addition, the acidic environment in the tissue of stroke victims necessitates drug release upon pH stimuli. Here, we report the encapsulation of single neural stem cells (NSCs) using a layer-by-layer (LbL) self-assembly technique with polyelectrolytes gelatin and alginate. Analysis of the NSCs showed that the LbL encapsulation would not affect the viability, proliferation, or differentiation of the cells. When insulin-like growth factor-1 (IGF-1) was loaded on the coating material alginate, its release from alginate into the medium presented in a time-dependent and pH-dependent way. IGF-1 significantly enhanced the proliferation of the encapsulated NSCs, demonstrating a drug-carrier function of the LbL single-cell nanocoating. It provided a potential treatment strategy for nervous system disorders such as stroke.
authors.affiliation: Department of Neurosurgery, Southwest Hospital, Third Military Medical University , 30 Gaotanyan Street, Chongqing 400038, China.
authors.forename: Wenyan
authors.initials: W
authors.lastname: Li
authors.forename: Teng
authors.initials: T
authors.lastname: Guan
authors.forename: Xiaosha
authors.initials: X
authors.lastname: Zhang
authors.forename: Ziyuan
authors.initials: Z
authors.lastname: Wang
authors.forename: Meng
authors.initials: M
authors.lastname: Wang
authors.forename: Wen
authors.initials: W
authors.lastname: Zhong
authors.forename: Hua
authors.initials: H
authors.lastname: Feng
authors.forename: Malcolm
authors.initials: M
authors.lastname: Xing
authors.forename: Jiming
authors.initials: J
authors.lastname: Kong
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology; D049109:Cell Proliferation / Q000187:drug effects / Q000502:physiology; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D007334:Insulin-Like Growth Factor I / Q000737:chemistry / Q000494:pharmacology*; D008422:Materials Testing; D058953:Neural Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000502:physiology*; D009474:Neurons / Q000166:cytology* / Q000187:drug effects / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D054457:Tissue Scaffolds
chemical_list: D020099:Coated Materials, Biocompatible; D007334:Insulin-Like Growth Factor I
keywords: controlled release; layer-by-layer; neural stem cells; pH responsive; single-cell encapsulation; stem cell microenvironment