title: Counting of Escherichia coli by a microflow cytometer based on a photonic-microfluidic integrated device.
abstract: Counting of Escherichia coli DH5α-cell suspensions in PBS is performed using a microflow cytometer based on a photonic-microfluidic integrated device. Side-scattered light signals are used to count the E. coli cells. A detection efficiency of 92% is achieved when compared with the expected count from a hemocytometer. The detection efficiency is correlated to the ratio of sample to sheath flow rates. It is demonstrated that E. coli can be easily distinguished from beads of similar sizes (2-4 μm) as their scattering intensities are different.
authors.affiliation: School of Biomedical Engineering, McMaster University, Hamilton, Canada; Institute of Microelectronics, Chinese Academy of Science, Beijing, China.
authors.forename: Tianyi
authors.initials: T
authors.lastname: Guo
authors.forename: Yin
authors.initials: Y
authors.lastname: Wei
authors.forename: Changqing
authors.initials: C
authors.lastname: Xu
authors.forename: Benjamin R
authors.initials: BR
authors.lastname: Watts
authors.forename: Zhiyi
authors.initials: Z
authors.lastname: Zhang
authors.forename: Qiyin
authors.initials: Q
authors.lastname: Fang
authors.forename: Haiying
authors.initials: H
authors.lastname: Zhang
authors.forename: P Ravi
authors.initials: PR
authors.lastname: Selvaganapathy
authors.forename: M Jamal
authors.initials: MJ
authors.lastname: Deen
mesh_terms: D001431:Bacteriological Techniques / Q000295:instrumentation / Q000379:methods; D004867:Equipment Design; D004926:Escherichia coli; D005434:Flow Cytometry / Q000295:instrumentation* / Q000379:methods*; D046210:Microfluidic Analytical Techniques; D011137:Polystyrenes
chemical_list: D011137:Polystyrenes
keywords: Escherichia coli; Integrated optics; Microflow cytometer; Scattering detection