title: Conjugative reporter system for the use in Bacillus licheniformis and closely related Bacilli.
abstract: Bacillus wild-type strains are genetically difficult to manipulate, and thus, the options for rational strain investigation and design are limited. Here, we present a set of small conjugative shuttle vectors for the use in Bacillus licheniformis and related, genetically difficult accessible wild-type strains. The vector set comprises the modular general-purpose vector pV2 and its derivatives pV3SDlacZ and pV3lacZ. The pV3 vectors are designed for the investigation of transcriptional and translational activities of regulatory regions like promoters and ribosomal binding sites (RBS). The vector set has been tested for investigating gene regulation under aerobic and anaerobic conditions.
authors.affiliation: Department of Genomic and Applied Microbiology, Institute of Microbiology and Genetics, Georg-August University of Göttingen, Göttingen, Germany.
authors.forename: R
authors.initials: R
authors.lastname: Hertel
authors.affiliation: Department of Genomic and Applied Microbiology, Institute of Microbiology and Genetics, Georg-August University of Göttingen, Göttingen, Germany.
authors.forename: S
authors.initials: S
authors.lastname: Volland
authors.affiliation: Department of Genomic and Applied Microbiology, Institute of Microbiology and Genetics, Georg-August University of Göttingen, Göttingen, Germany.
authors.forename: H
authors.initials: H
authors.lastname: Liesegang
mesh_terms: D001407:Bacillus / Q000235:genetics*; D003227:Conjugation, Genetic; D004926:Escherichia coli / Q000235:genetics; D017930:Genes, Reporter; D005822:Genetic Vectors; D007763:Lac Operon; D010957:Plasmids; D011401:Promoter Regions, Genetic
keywords: Bacillus licheniformis DSM13; Firmicutes; Shuttle vector; beta-galactosidase; lacZ; pV2; pV3SDlacZ; pV3lacZ