title: Mouse adipose tissue stromal cells give rise to skeletal and cardiomyogenic cell sub-populations.
abstract: We previously reported that adipose tissue could generate cardiomyocyte-like cells from crude stromal vascular fraction (SVF) in vitro that improved cardiac function in a myocardial infarction context. However, it is not clear whether these adipose-derived cardiomyogenic cells (AD-CMG) constitute a homogenous population and if AD-CMG progenitors could be isolated as a pure population from the SVF of adipose tissue. This study aims to characterize the different cell types that constitute myogenic clusters and identify the earliest AD-CMG progenitors in vitro for establishing a complete phenotype and use it to sort AD-CMG progenitors from crude SVF. Here, we report cell heterogeneity among adipose-derived clusters during their course of maturation and highlighted sub-populations that exhibit original mixed cardiac/skeletal muscle phenotypes with a progressive loss of cardiac phenotype with time in liquid culture conditions. Moreover, we completed the phenotype of AD-CMG progenitors but we failed to sort them from the SVF. We demonstrated that micro-environment is required for the maturation of myogenic phenotype by co-culture experiments. These findings bring complementary data on AD-CMG and suggest that their emergence results from in vitro events.
authors.affiliation: Centre National de la Recherche Scientifique (CNRS) UMR 5273, STROMALab Toulouse, France ; Université Paul Sabatier de Toulouse Toulouse, France ; Institut National de la Santé et de la Recherche Médical (INSERM) U1031, STROMALab Toulouse, France ; Etablissement Français du Sang (EFS) Pyrénées-Méditerranée, STROMALab Toulouse, France.
authors.forename: Cécile
authors.initials: C
authors.lastname: Dromard
authors.affiliation: Centre National de la Recherche Scientifique (CNRS) UMR 5273, STROMALab Toulouse, France ; Université Paul Sabatier de Toulouse Toulouse, France ; Institut National de la Santé et de la Recherche Médical (INSERM) U1031, STROMALab Toulouse, France ; Etablissement Français du Sang (EFS) Pyrénées-Méditerranée, STROMALab Toulouse, France.
authors.forename: Corinne
authors.initials: C
authors.lastname: Barreau
authors.affiliation: Centre National de la Recherche Scientifique (CNRS) UMR 5273, STROMALab Toulouse, France ; Institut National de la Santé et de la Recherche Médical (INSERM) U1031, STROMALab Toulouse, France ; Etablissement Français du Sang (EFS) Pyrénées-Méditerranée, STROMALab Toulouse, France.
authors.forename: Mireille
authors.initials: M
authors.lastname: André
authors.affiliation: Centre National de la Recherche Scientifique (CNRS) UMR 5273, STROMALab Toulouse, France ; Institut National de la Santé et de la Recherche Médical (INSERM) U1031, STROMALab Toulouse, France ; Etablissement Français du Sang (EFS) Pyrénées-Méditerranée, STROMALab Toulouse, France.
authors.forename: Sandra
authors.initials: S
authors.lastname: Berger-Müller
authors.affiliation: Centre National de la Recherche Scientifique (CNRS) UMR 5273, STROMALab Toulouse, France ; Université Paul Sabatier de Toulouse Toulouse, France ; Institut National de la Santé et de la Recherche Médical (INSERM) U1031, STROMALab Toulouse, France ; Etablissement Français du Sang (EFS) Pyrénées-Méditerranée, STROMALab Toulouse, France.
authors.forename: Louis
authors.initials: L
authors.lastname: Casteilla
authors.affiliation: Centre National de la Recherche Scientifique (CNRS) UMR 5273, STROMALab Toulouse, France ; Université Paul Sabatier de Toulouse Toulouse, France ; Institut National de la Santé et de la Recherche Médical (INSERM) U1031, STROMALab Toulouse, France ; Etablissement Français du Sang (EFS) Pyrénées-Méditerranée, STROMALab Toulouse, France.
authors.forename: Valerie
authors.initials: V
authors.lastname: Planat-Benard
keywords: Adipose tissue stem cells; cardiac and skeletal myogenesis; cell plasticity; in vitro differentiation