title: Effect of distillers feedstuffs and lasalocid on Campylobacter carriage in feedlot cattle.
abstract: Campylobacter bacteria are foodborne pathogens that can colonize the gut of food animals. Limited in their ability to ferment sugars, Campylobacter can derive energy for growth via amino acid catabolism. The objectives of the present studies were to test whether supplemental distillers grains containing high amounts of rumen-undegradable intake protein or supplemental lasalocid may, by promoting amino acid flow to the lower bovine gut, increase intestinal carriage of Campylobacter. In study one, 10 steers (5 per treatment) were adapted to diets formulated to achieve 0 or 30% dried distillers grains. After an initial 14-day adaptation to the basal diet, control and treated steers were fed their respective diets for 23 days, after which time they were fed supplemental lasalocid for an additional 8 days, followed by a 5-day withdrawal. In study two, 24 steers preacclimated to a basal diet were adapted via 3-day periodic increases to dietary treatments formulated to achieve 0, 30, or 60% wet corn distillers grains with solubles. Analysis of Campylobacter bacteria cultured from duodenal and fecal samples in study one and from fecal samples in study two revealed no effect of dried distillers grains or wet corn distillers grains with solubles on the prevalence or concentrations of duodenal or fecal Campylobacter. The results from study one indicated that colonized steers, regardless of treatment, harbored higher Campylobacter concentrations when transitioned to the basal diet than when coming off pasture. Campylobacter carriage was unaffected by lasalocid. These results provide no evidence that feeding distillers grains high in rumen-undegradable intake protein or supplemental lasalocid contributes to increased intestinal carriage of Campylobacter in fed cattle.
authors.affiliation: U.S. Department of Agriculture, Agricultural Research Service, Southern Plains Agricultural Research Center, Food & Feed Safety Research Unit, 2881 F&B Road, College Station, Texas 77845, USA. robin.anderson@ars.usda.gov.
authors.forename: Robin C
authors.initials: RC
authors.lastname: Anderson
authors.affiliation: U.S. Department of Agriculture, Agricultural Research Service, Southern Plains Agricultural Research Center, Food & Feed Safety Research Unit, 2881 F&B Road, College Station, Texas 77845, USA.
authors.forename: Roger B
authors.initials: RB
authors.lastname: Harvey
authors.affiliation: Animal Science Department, Texas A&M University, College Station, Texas 77843, USA.
authors.forename: Tryon A
authors.initials: TA
authors.lastname: Wickersham
authors.affiliation: Feedlot Research Group, West Texas A&M University, Canyon, Texas 79016, USA; Texas AgriLife Research, 6500 Amarillo Boulevard West, Amarillo, Texas 79106, C220 Animal Science, University of Nebraska-Lincoln, Lincoln, NE 68583-0908, USA.
authors.forename: Jim C
authors.initials: JC
authors.lastname: MacDonald
authors.affiliation: Feedlot Research Group, West Texas A&M University, Canyon, Texas 79016, USA; Departamento de Ciencias de la Vida, Universidad de las Fuerzas Armadas-ESPE, Sangolqui 171-5-2318, Ecuador.
authors.forename: Christian H
authors.initials: CH
authors.lastname: Ponce
authors.affiliation: Feedlot Research Group, West Texas A&M University, Canyon, Texas 79016, Texas AgriLife Research, 6500 Amarillo Boulevard West, Amarillo, Texas 79106, USA; Global Animal Products, 3701 Airway Boulevard, Amarillo, TX 79118, USA.
authors.forename: Mike
authors.initials: M
authors.lastname: Brown
authors.affiliation: Texas AgriLife Research, P.O. Box 1658, Vernon, Texas 76385, USA.
authors.forename: William E
authors.initials: WE
authors.lastname: Pinchak
authors.affiliation: Texas AgriLife Research, 6500 Amarillo Boulevard West, Amarillo, Texas 79106, USA; Texas AgriLife Research, P.O. Box 1658, Vernon, Texas 76385, USA; Zoetis, 333 Portage Street, Kalamazoo, MI 49007, USA.
authors.forename: Jason B
authors.initials: JB
authors.lastname: Osterstock
authors.affiliation: U.S. Department of Agriculture, Agricultural Research Service, Southern Plains Agricultural Research Center, Food & Feed Safety Research Unit, 2881 F&B Road, College Station, Texas 77845, USA.
authors.forename: Nathan
authors.initials: N
authors.lastname: Krueger
authors.affiliation: U.S. Department of Agriculture, Agricultural Research Service, Southern Plains Agricultural Research Center, Food & Feed Safety Research Unit, 2881 F&B Road, College Station, Texas 77845, USA.
authors.forename: David J
authors.initials: DJ
authors.lastname: Nisbet
mesh_terms: D000821:Animal Feed / Q000032:analysis*; D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis / Q000494:pharmacology; D002167:Campylobacter / Q000187:drug effects / Q000254:growth & development*; D002417:Cattle / Q000378:metabolism / Q000382:microbiology*; D004032:Diet / Q000662:veterinary; D005243:Feces / Q000382:microbiology; D041981:Gastrointestinal Tract / Q000382:microbiology; D007832:Lasalocid / Q000032:analysis* / Q000494:pharmacology; D003313:Zea mays / Q000737:chemistry*
chemical_list: D000900:Anti-Bacterial Agents; D007832:Lasalocid