title: [Effects of lentivirus-mediated epidermal growth factor-like domain 7 silencing on proliferation and invasion of human laryngeal carcinoma Hep-2 cells].
abstract: OBJECTIVE
  To explore the effects of epidermal growth factor-like domain 7 (EGFL7) gene silencing on the proliferation and invasion ablity of laryngeal carcinoma cells.
  
  
  METHODS
  A lentiviral vector expressing EGFL7 shRNA was constructed and transfected into human laryngeal carcinoma Hep-2 cells. The expressions of EGFL7 mRNA and protein were detected by Real-time PCR and Western blot, respectively. Cell proliferation was evaluated by CCK-8 assay, cell cycle and apoptosis were tested by flow cytometry, and cell invasion was detected by transwell invasion assay.
  
  
  RESULTS
  The relative expression level s of EGFL7 mRNA and protein in EGFL7-SuRNA group were svgnificantly lower than control group (P < 0.001). Western blot analysis proved that the relative expression of EGFL7 protein in NC group, Lenti-NC group and Lenti-EGFL7 group was (0.39 ± 0.12),(0.36 ± 0.14) and (0.07 ± 0.04), respectively. EGFL7 expression in Lenri-EGFL7 group was significantly inhibited than NC group (P < 0.001), which confirmed that the recombinant lentivirus was successfully transfected into Hep-2 cells. The proliferation of Hep-2 cells was significantly inhibited after transfection (P < 0.01). Compared with the NC group and Lenti-NC group, the proportion of cells in S phase was significantly increased in Lenti-EGFL7 group (P < 0.01), and the proportion in G1 phase was significantly decreased (P < 0.05). Cell apoptosis assay showed that the apoptotic rate in Lenti-EGFL7 group (66.2 ± 1.28) % was significantly increased in NC group (6.09 ± 3.28) % and Lenti-NC group (9.86 ± 2.13) %. In Transwell invision assay, the mean number of cells coming through the Metrigel in Lenti-EGFL7 group was significantly decreased than in the NC group and Lenti-NC group (P < 0.01).
  
  
  CONCLUSIONS
  The proliferation and invasion ablity of laryngeal carcinoma Hep-2 cells can be inhibited by siRNA mediated EGFL7 gene silencing.
authors.affiliation: Department of Otorhinolaryngology Head and Neck Surgery, Third Affiliated Hospital of SUN Yat-sen University, Guangzhou 510630, China.
authors.forename: Jingjia
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Otorhinolaryngology Head and Neck Surgery, Third Affiliated Hospital of SUN Yat-sen University, Guangzhou 510630, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Ye
authors.affiliation: Department of Otorhinolaryngology Head and Neck Surgery, Third Affiliated Hospital of SUN Yat-sen University, Guangzhou 510630, China.
authors.forename: Shimin
authors.initials: S
authors.lastname: Zhuang
authors.affiliation: Department of Otorhinolaryngology Head and Neck Surgery, Third Affiliated Hospital of SUN Yat-sen University, Guangzhou 510630, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Wang
authors.affiliation: Department of Otorhinolaryngology Head and Neck Surgery, Third Affiliated Hospital of SUN Yat-sen University, Guangzhou 510630, China.
authors.forename: Zhiyuan
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Department of Otorhinolaryngology Head and Neck Surgery, Third Affiliated Hospital of SUN Yat-sen University, Guangzhou 510630, China.
authors.forename: Lihong
authors.initials: L
authors.lastname: Chang
authors.affiliation: Department of Otorhinolaryngology Head and Neck Surgery, Third Affiliated Hospital of SUN Yat-sen University, Guangzhou 510630, China. Email: gehuazh@hotmail.com.
authors.forename: Gehua
authors.initials: G
authors.lastname: Zhang
mesh_terms: D017209:Apoptosis; D002453:Cell Cycle; D002455:Cell Division; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D066255:EGF Family of Proteins; D020868:Gene Silencing; D005822:Genetic Vectors; D056945:Hep G2 Cells; D006801:Humans; D007822:Laryngeal Neoplasms / Q000378:metabolism / Q000821:virology*; D016086:Lentivirus; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D014162:Transfection
chemical_list: D066255:EGF Family of Proteins; D012333:RNA, Messenger; D034741:RNA, Small Interfering