title: Endothelial damage and vascular calcification in patients with chronic kidney disease.
abstract: Vascular calcification (VC) is a frequent complication of chronic kidney disease (CKD) and is a predictor of cardiovascular morbidity and mortality. In the present study, we investigated the potential involvement of endothelial microparticles (MPs) and endothelial progenitor cells (EPCs) in the generation of VC in CKD patients. The number of circulating EMPs is greater in patients with VC than without VC (307 ± 167 vs. 99 ± 75 EMPs/μl, P < 0.001). The percentage of EPCs is significantly lower in patient with VC than in patients without VC (0.14 ± 0.11% vs. 0.25 ± 0.18%, P = 0.002). The number of EPCs expressing osteocalcin (OCN) was higher in VC patients (349 ± 63 cells/100,000) than in non-VC patients (139 ± 75 cells/100,000, P < 0.01). In vitro, MPs obtained from CKD patients were able to induce OCN expression in EPCs from healthy donors; the increase in OCN expression was more accentuated if MPs were obtained from CKD patients with VC. MPs from CKD patients also induced OCN expression in vascular smooth muscle cells and fibroblasts. In CKD patients, the rise in endothelial MPs associated with a decrease in the number of EPCs, suggesting an imbalance in the processes of endothelial damage and repair in CKD patients, mainly those with VC. Our results suggest that EPCs, through OCN expression, may directly participate in the process of VC.
authors.affiliation: Instituto Maimónides de Investigación Biomédica de Córdoba, Reina Sofía University Hospital, University of Córdoba, Córdoba, Spain; Nephrology Unit, Reina Sofía University Hospital, Córdoba, Spain; RETICs Red Renal (Instituto de Salud Carlos III), Madrid, Spain; and.
authors.forename: Sagrario
authors.initials: S
authors.lastname: Soriano
authors.affiliation: Instituto Maimónides de Investigación Biomédica de Córdoba, Reina Sofía University Hospital, University of Córdoba, Córdoba, Spain; Nephrology Unit, Reina Sofía University Hospital, Córdoba, Spain; RETICs Red Renal (Instituto de Salud Carlos III), Madrid, Spain; and.
authors.forename: Andrés
authors.initials: A
authors.lastname: Carmona
authors.affiliation: Radiology Unit, Reina Sofía University Hospital, Córdoba, Spain;
authors.forename: Francisco
authors.initials: F
authors.lastname: Triviño
authors.affiliation: Instituto Maimónides de Investigación Biomédica de Córdoba, Reina Sofía University Hospital, University of Córdoba, Córdoba, Spain; Nephrology Unit, Reina Sofía University Hospital, Córdoba, Spain; RETICs Red Renal (Instituto de Salud Carlos III), Madrid, Spain; and.
authors.forename: Mariano
authors.initials: M
authors.lastname: Rodriguez
authors.affiliation: Radiology Unit, Reina Sofía University Hospital, Córdoba, Spain;
authors.forename: Marina
authors.initials: M
authors.lastname: Alvarez-Benito
authors.affiliation: Instituto Maimónides de Investigación Biomédica de Córdoba, Reina Sofía University Hospital, University of Córdoba, Córdoba, Spain; Nephrology Unit, Reina Sofía University Hospital, Córdoba, Spain; RETICs Red Renal (Instituto de Salud Carlos III), Madrid, Spain; and.
authors.forename: Alejandro
authors.initials: A
authors.lastname: Martín-Malo
authors.affiliation: Instituto Maimónides de Investigación Biomédica de Córdoba, Reina Sofía University Hospital, University of Córdoba, Córdoba, Spain; Nephrology Unit, Reina Sofía University Hospital, Córdoba, Spain; RETICs Red Renal (Instituto de Salud Carlos III), Madrid, Spain; and.
authors.forename: Maria-Antonia
authors.initials: MA
authors.lastname: Alvarez-Lara
authors.affiliation: Instituto Maimónides de Investigación Biomédica de Córdoba, Reina Sofía University Hospital, University of Córdoba, Córdoba, Spain; RETICs Red Renal (Instituto de Salud Carlos III), Madrid, Spain; and Biologia de Sistemas Department Módulo II-Planta B, Alcala de Henares University, Madrid, Spain.
authors.forename: Rafael
authors.initials: R
authors.lastname: Ramírez
authors.affiliation: Instituto Maimónides de Investigación Biomédica de Córdoba, Reina Sofía University Hospital, University of Córdoba, Córdoba, Spain; Nephrology Unit, Reina Sofía University Hospital, Córdoba, Spain; RETICs Red Renal (Instituto de Salud Carlos III), Madrid, Spain; and.
authors.forename: Pedro
authors.initials: P
authors.lastname: Aljama
authors.affiliation: Instituto Maimónides de Investigación Biomédica de Córdoba, Reina Sofía University Hospital, University of Córdoba, Córdoba, Spain; Nephrology Unit, Reina Sofía University Hospital, Córdoba, Spain; RETICs Red Renal (Instituto de Salud Carlos III), Madrid, Spain; and julia.carracedo.exts@juntadeandalucia.es.
authors.forename: Julia
authors.initials: J
authors.lastname: Carracedo
mesh_terms: D000368:Aged; D017304:Annexin A5 / Q000096:biosynthesis / Q000235:genetics; D002114:Calcinosis / Q000378:metabolism / Q000473:pathology*; D002196:Capillaries / Q000502:physiology; D055252:Cell-Derived Microparticles / Q000378:metabolism / Q000473:pathology; D004730:Endothelium, Vascular / Q000378:metabolism / Q000473:pathology*; D005260:Female; D005347:Fibroblasts / Q000378:metabolism; D061307:Human Umbilical Vein Endothelial Cells / Q000378:metabolism / Q000473:pathology; D006801:Humans; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000378:metabolism; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000096:biosynthesis / Q000235:genetics; D061251:Primary Cell Culture; D051436:Renal Insufficiency, Chronic / Q000378:metabolism / Q000473:pathology*; D013234:Stem Cells / Q000473:pathology
chemical_list: D017304:Annexin A5; D019408:Platelet Endothelial Cell Adhesion Molecule-1
keywords: chronic kidney disease; endothelial damage; endothelial microparticles; endothelial progenitors cells; vascular calcification