title: Evaluation of antibacterial activity of Prosopis juliflora (SW.) DC. leaves.
abstract: BACKGROUND
  The ethnobotanical importance of Prosopis juliflora is well-known in the folkloric system of medicine for the treatment of various ailments. Although, the study related to the antibacterial potential of this plant, from Central India is scanty.
  
  
  METHODS
  The in vitro antibacterial activity of Prosopis juliflora leaves collected from the local area was evaluated against ten bacterial type cultures by agar well diffusion assay. The crude extracts prepared by two methods separately with three different solvents were examined for the preliminary antibacterial activity and phytochemical screening, the results of which were used for the choice of solvent and mass extraction of crude extract. Solvent fractionation of crude extract was done employing two sets of solvents namely Set-PCE and Set-HDB which resulted in total, six organic and two aqueous fractions, which were finally subjected to antibacterial activities.
  
  
  RESULTS
  Varying degrees of growth inhibition was shown by all the fractions against tested microorganisms. The highest antibacterial activity was observed in aqueous fractions as compared to solvent fractions.
  
  
  CONCLUSIONS
  Isolation and characterization of the bioactive components can be further done by systematic screening of the most active solvent fraction which could lead to the possible source of new antibacterial agents.
authors.affiliation: Biochemical Research Laboratory, Centre for Scientific Research & Development, People's University, Bhopal - 462037, Madhya Pradesh, India.
authors.forename: Rupesh
authors.initials: R
authors.lastname: Thakur
authors.affiliation: Biochemical Research Laboratory, Centre for Scientific Research & Development, People's University, Bhopal - 462037, Madhya Pradesh, India.
authors.forename: Rupal
authors.initials: R
authors.lastname: Singh
authors.affiliation: Biotechnology & Pharmacology Laboratory, Centre for Scientific Research & Development, People's University, Bhopal - 462037, Madhya Pradesh, India.
authors.forename: Pooja
authors.initials: P
authors.lastname: Saxena
authors.affiliation: Biotechnology & Pharmacology Laboratory, Centre for Scientific Research & Development, People's University, Bhopal - 462037, Madhya Pradesh, India.
authors.forename: Abin
authors.initials: A
authors.lastname: Mani
mesh_terms: D000900:Anti-Bacterial Agents / Q000302:isolation & purification / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects / Q000254:growth & development; D004353:Drug Evaluation, Preclinical; D007194:India; D008826:Microbial Sensitivity Tests; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry; D029862:Prosopis / Q000737:chemistry*
chemical_list: D000900:Anti-Bacterial Agents; D010936:Plant Extracts
keywords: antibacterial activity; herbal extract; phytochemical screening; solvent fractionation