title: miR-21 improves the neurological outcome after traumatic brain injury in rats.
abstract: The expression levels of microRNAs (miRNAs) including miR-21, have been reported to change in response to traumatic brain injury (TBI), suggesting that they may influence the pathophysiological process in brain injury. To analyze the potential effect of miR-21 on neurological function after TBI, we employed the fluid percussion injury rat model and manipulated the expression level of miR-21 in brain using intracerebroventricular infusion of miR-21 agomir or antagomir. We found that upregulation of miR-21 level in brain conferred a better neurological outcome after TBI by improving long-term neurological function, alleviating brain edema and decreasing lesion volume. To further investigate the mechanism underlying this protective effect, we evaluated the impact of miR-21 on apoptosis and angiogenesis in brain after TBI. We found that miR-21 inhibited apoptosis and promoted angiogenesis through regulating the expression of apoptosis- and angiogenesis-related molecules. In addition, the expression of PTEN, a miR-21 target gene, was inhibited and Akt signaling was activated in the procedure. Taken together, these data indicate that miR-21 could be a potential therapeutic target for interventions after TBI.
authors.affiliation: 1] Laboratory of Neuro-Trauma, Tianjin Neurological Institute, Tianjin, China [2] Key Laboratory of Post-trauma Neuro-repair and Regeneration in Central Nervous System, Ministry of Education, Tianjin, China; Tianjin Key Laboratory of Injuries, Variations and Regeneration of Nervous System, Tianjin, Tianjin, China [3] Department of Neurosurgery, Tianjin Medical University General Hospital, Tianjin, China.
authors.forename: Xin-Tong
authors.initials: XT
authors.lastname: Ge
authors.affiliation: 1] Key Laboratory of Post-trauma Neuro-repair and Regeneration in Central Nervous System, Ministry of Education, Tianjin, China; Tianjin Key Laboratory of Injuries, Variations and Regeneration of Nervous System, Tianjin, Tianjin, China [2] Department of Neurosurgery, Tianjin Medical University General Hospital, Tianjin, China [3] Department of Geriatrics, Tianjin Medical University General Hospital, Tianjin, China; Laboratory of Neuro-Trauma and Neurodegenerative Disorders, Tianjin Geriatrics Institute, Tianjin, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Lei
authors.affiliation: Department of Neurology, Duke University School of Medicine, Durham, North Carolina, U.S.A.
authors.forename: Hai-Chen
authors.initials: HC
authors.lastname: Wang
authors.affiliation: 1] Key Laboratory of Post-trauma Neuro-repair and Regeneration in Central Nervous System, Ministry of Education, Tianjin, China; Tianjin Key Laboratory of Injuries, Variations and Regeneration of Nervous System, Tianjin, Tianjin, China [2] Department of Neurosurgery, Tianjin Medical University General Hospital, Tianjin, China [3] Laboratory of Neuro-Oncology, Tianjin Neurological Institute, Tianjin, China.
authors.forename: An-Ling
authors.initials: AL
authors.lastname: Zhang
authors.affiliation: 1] Laboratory of Neuro-Trauma, Tianjin Neurological Institute, Tianjin, China [2] Key Laboratory of Post-trauma Neuro-repair and Regeneration in Central Nervous System, Ministry of Education, Tianjin, China; Tianjin Key Laboratory of Injuries, Variations and Regeneration of Nervous System, Tianjin, Tianjin, China [3] Department of Geriatrics, Tianjin Medical University General Hospital, Tianjin, China; Laboratory of Neuro-Trauma and Neurodegenerative Disorders, Tianjin Geriatrics Institute, Tianjin, China.
authors.forename: Zhao-Li
authors.initials: ZL
authors.lastname: Han
authors.affiliation: 1] Laboratory of Neuro-Trauma, Tianjin Neurological Institute, Tianjin, China [2] Key Laboratory of Post-trauma Neuro-repair and Regeneration in Central Nervous System, Ministry of Education, Tianjin, China; Tianjin Key Laboratory of Injuries, Variations and Regeneration of Nervous System, Tianjin, Tianjin, China [3] Department of Neurosurgery, Tianjin Medical University General Hospital, Tianjin, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Chen
authors.affiliation: 1] Laboratory of Neuro-Trauma, Tianjin Neurological Institute, Tianjin, China [2] Key Laboratory of Post-trauma Neuro-repair and Regeneration in Central Nervous System, Ministry of Education, Tianjin, China; Tianjin Key Laboratory of Injuries, Variations and Regeneration of Nervous System, Tianjin, Tianjin, China [3] Department of Neurosurgery, Tianjin Medical University General Hospital, Tianjin, China.
authors.forename: Sheng-Hui
authors.initials: SH
authors.lastname: Li
authors.affiliation: 1] Laboratory of Neuro-Trauma, Tianjin Neurological Institute, Tianjin, China [2] Key Laboratory of Post-trauma Neuro-repair and Regeneration in Central Nervous System, Ministry of Education, Tianjin, China; Tianjin Key Laboratory of Injuries, Variations and Regeneration of Nervous System, Tianjin, Tianjin, China [3] Department of Neurosurgery, Tianjin Medical University General Hospital, Tianjin, China.
authors.forename: Rong-Cai
authors.initials: RC
authors.lastname: Jiang
authors.affiliation: 1] Key Laboratory of Post-trauma Neuro-repair and Regeneration in Central Nervous System, Ministry of Education, Tianjin, China; Tianjin Key Laboratory of Injuries, Variations and Regeneration of Nervous System, Tianjin, Tianjin, China [2] Department of Neurosurgery, Tianjin Medical University General Hospital, Tianjin, China [3] Laboratory of Neuro-Oncology, Tianjin Neurological Institute, Tianjin, China.
authors.forename: Chun-Sheng
authors.initials: CS
authors.lastname: Kang
authors.affiliation: 1] Laboratory of Neuro-Trauma, Tianjin Neurological Institute, Tianjin, China [2] Key Laboratory of Post-trauma Neuro-repair and Regeneration in Central Nervous System, Ministry of Education, Tianjin, China; Tianjin Key Laboratory of Injuries, Variations and Regeneration of Nervous System, Tianjin, Tianjin, China [3] Department of Neurosurgery, Tianjin Medical University General Hospital, Tianjin, China.
authors.forename: Jian-Ning
authors.initials: JN
authors.lastname: Zhang
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000235:genetics; D051017:Apoptosis Regulatory Proteins / Q000235:genetics / Q000378:metabolism; D001253:Astrocytes / Q000378:metabolism; D001930:Brain Injuries / Q000235:genetics* / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D004195:Disease Models, Animal; D015870:Gene Expression; D007150:Immunohistochemistry; D008297:Male; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D017628:Microglia / Q000378:metabolism; D018919:Neovascularization, Physiologic / Q000235:genetics; D009474:Neurons / Q000378:metabolism; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D051381:Rats; D015398:Signal Transduction
chemical_list: D051017:Apoptosis Regulatory Proteins; D035683:MicroRNAs; C546082:mirn21 microRNA, rat; D051057:Proto-Oncogene Proteins c-akt