title: Overproduction of the MtrCDE efflux pump in Neisseria gonorrhoeae produces unexpected changes in cellular transcription patterns.
abstract: The global consequence of drug efflux gene overexpression in bacteria has not been specifically analyzed because strains showing high-level expression typically have mutations in genes encoding regulatory proteins that control other genes. Results from a transcriptional profiling study performed with a strain of Neisseria gonorrhoeae that is capable of high-level transcription of the mtrCDE efflux pump operon independently of control by cognate regulatory proteins revealed that its overexpression has ramifications for systems other than drug efflux.
authors.affiliation: Department of Microbiology and Immunology, Emory University School of Medicine, Atlanta, Georgia, USA.
authors.forename: Elizabeth A
authors.initials: EA
authors.lastname: Ohneck
authors.affiliation: Department of Microbiology and Immunology, Emory University School of Medicine, Atlanta, Georgia, USA.
authors.forename: Maira
authors.initials: M
authors.lastname: Goytia
authors.affiliation: Department of Microbiology and Immunology, Emory University School of Medicine, Atlanta, Georgia, USA.
authors.forename: Corinne E
authors.initials: CE
authors.lastname: Rouquette-Loughlin
authors.affiliation: Department of Medicine, Emory University School of Medicine, Atlanta, Georgia, USA.
authors.forename: Sandeep J
authors.initials: SJ
authors.lastname: Joseph
authors.affiliation: Department of Medicine, Emory University School of Medicine, Atlanta, Georgia, USA.
authors.forename: Timothy D
authors.initials: TD
authors.lastname: Read
authors.affiliation: Department of Microbiology and Immunology, F. Edward Herbert School of Medicine, Uniformed Services University of the Health Sciences, Bethesda, Maryland, USA.
authors.forename: Ann E
authors.initials: AE
authors.lastname: Jerse
authors.affiliation: Department of Microbiology and Immunology, Emory University School of Medicine, Atlanta, Georgia, USA Laboratories of Bacterial Pathogenesis, VA Medical Center, Decatur, Georgia, USA wshafer@emory.edu.
authors.forename: William M
authors.initials: WM
authors.lastname: Shafer
mesh_terms: D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial / Q000235:genetics / Q000502:physiology; D019450:Genes, MDR / Q000235:genetics / Q000502:physiology*; D009344:Neisseria gonorrhoeae / Q000235:genetics / Q000378:metabolism*; D060888:Real-Time Polymerase Chain Reaction; D014158:Transcription, Genetic / Q000235:genetics / Q000502:physiology