title: Preparation of poly(vinylphenylboronic acid) chain grafted poly(glycidylmethacrylate-co-ethylenedimethacrylate) beads for the selective enrichment of glycoprotein.
abstract: Surface-initiated atom transfer radical polymerization was successfully used to prepare 4-vinylphenylboronic acid functionalized poly(glycidylmethacrylate-co-ethylenedimethacrylate) beads for the selective enrichment of glycoprotein from complex biological samples in this study. The modified bead surfaces were characterized using Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy. The sorption behaviors, including adsorption isotherms, incubation time, and pH effect, were investigated. The results demonstrated that the boronated beads have a high affinity for glycoprotein, which is due to the well-defined boronic acid brushes on the beads surfaces. Furthermore, the polyvinylphenylboronic acid grafted poly(glycidylmethacrylate-co-ethylenedimethacrylate) beads were used to efficiently enrich and purify glycoprotein from real egg white samples and α-fetoprotein from human serum samples. The mass spectrometry results demonstrated that the polyvinylphenylboronic acid grafted poly(glycidylmethacrylate-co-ethylenedimethacrylate) beads are a suitable material for the enrichment of glycosylated protein from complex biological samples.
authors.affiliation: Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an, P. R. China.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.forename: Yan-li
authors.initials: YL
authors.lastname: Ni
authors.forename: Xiao-ling
authors.initials: XL
authors.lastname: Zheng
mesh_terms: D000327:Adsorption; D053000:Analytic Sample Preparation Methods / Q000379:methods*; D000818:Animals; D001897:Boronic Acids / Q000737:chemistry; D002645:Chickens; D004529:Egg White / Q000737:chemistry; D006023:Glycoproteins / Q000737:chemistry / Q000302:isolation & purification*; D006801:Humans; D008768:Methylmethacrylates / Q000737:chemistry*; D058105:Polymerization; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry*; D000509:alpha-Fetoproteins / Q000737:chemistry / Q000302:isolation & purification
chemical_list: D001897:Boronic Acids; D006023:Glycoproteins; D008768:Methylmethacrylates; D011108:Polymers; D000509:alpha-Fetoproteins; C020347:GMA-EDMA; C010686:benzeneboronic acid
keywords: Atom transfer radical polymerization; Boronic acid; Enrichment; Glycoprotein