title: Comparison of putative circulating cancer stem cell detection between the hepatic portal system and peripheral blood in colorectal cancer patients.
abstract: OBJECTIVE
  The present pilot study was conducted to detect putative cancer stem cell (CSC) from the hepatic portal system and peripheral blood in the colorectal cancer patients and to compare them to healthy donor and diverticulitis patients.
  
  
  METHODS
  Laboratory study was performed to identify the expression of cell surface markers, epithelial cell adhesion molecule (EpCAM), cytokeratin (CK) 18, CK20, CD44, and CD133, on several colon cancer cell lines. Clinical pilot study was conducted to detect putative circulating CSC as EpCAM(+)CD133(+) cell in colorectal cancer (n = 10), diverticulitis (n = 5), and four healthy donors, by using flow cytometry. Blood was drawn from the hepatic portal system and peripheral vein.
  
  
  RESULTS
  On laboratory study, EpCAM was expressed in whole colon cancer cell lines, and CD44 and CD133 were simultaneously expressed in 50% of the cell lines with stemness phenotype, but CK18 and CK20 were not expressed in most of the cell lines. On clinical study, the mean EpCAM(+)CD133(+) cell counts of 11.6/10(5) in the hepatic portal system were somewhat lower than 15.4/10(5) in peripheral vein (P = 0.241). As for diverticulitis patients, EpCAM(+)CD133(+) cells were also detected to have steeper dropped to near zero, after the surgery.
  
  
  CONCLUSIONS
  The numbers of putative CSC were not statistically different between the detection sites of the portal vein and peripheral vein in the colon cancer patients. Therefore, we may not have benefitted by getting the cells from the hepatic portal system. In addition, the CD133(+)EpCAM(+) cells in the colon cancer patients might contain normal stem cells from cancer inflammation similar to diverticulitis.
authors.affiliation: Department of Surgery, Pusan National University Yangsan Hospital, Yangsan, Korea. ; Research Institute for Convergence of Biomedical Science and Technology, Pusan National University Yangsan Hospital, Yangsan, Korea.
authors.forename: Byung Soo
authors.initials: BS
authors.lastname: Park
authors.affiliation: Department of Physiology, Pusan National University School of Medicine, Yangsan, Korea.
authors.forename: Seok Yun
authors.initials: SY
authors.lastname: Jung
authors.affiliation: Department of Physiology, Pusan National University School of Medicine, Yangsan, Korea.
authors.forename: Sang Mo
authors.initials: SM
authors.lastname: Kwon
authors.affiliation: Department of Biochemistry, Pusan National University School of Medicine, Yangsan, Korea.
authors.forename: Jae Ho
authors.initials: JH
authors.lastname: Bae
authors.affiliation: Department of Laboratory Medicine, Pusan National University Yangsan Hospital, Yangsan, Korea.
authors.forename: Sun Min
authors.initials: SM
authors.lastname: Lee
authors.affiliation: Department of Pathology, Pusan National University Yangsan Hospital, Yangsan, Korea.
authors.forename: Dong Hoon
authors.initials: DH
authors.lastname: Shin
authors.affiliation: Department of Surgery, Pusan National University Yangsan Hospital, Yangsan, Korea. ; Research Institute for Convergence of Biomedical Science and Technology, Pusan National University Yangsan Hospital, Yangsan, Korea.
authors.forename: Gyung Mo
authors.initials: GM
authors.lastname: Son
keywords: CD133; Cancer stem cell; Colorectal cancer; EpCAM; Hepatic portal system