title: Bioactive lignan constituents from the twigs of Lindera glauca.
abstract: A bioassay-guided fractionation and chemical investigation of the MeOH extract from the twigs of Lindera glauca (SIEB. et ZUCC.) BLUME resulted in the isolation and identification of six lignans (1-6) including three new lignan derivatives, named linderuca A (1), B (2), and C (3). The structures of the new compounds (1-3) were determined on the basis of spectroscopic analyses, including two dimensional NMR and circular dichroism (CD) spectroscopy studies. The cytotoxic activities of the isolates (1-6) were evaluated by determining their inhibitory effects on human tumor cell lines. Compounds 1-5 showed antiproliferative activities against A549, SK-OV-3, SK-MEL-2, and HCT-15 cell lines with IC50 values of 7.79-29.42 µM. Based on the understanding that inflammation is a crucial cause of tumor progression, we also investigated the anti-inflammatory activities of the isolates (1-6) in the lipopolysaccharide-stimulated murine microglia BV-2 cell line by measuring nitric oxide (NO) levels. The new lignans (1-3) significantly inhibited NO production with IC50 values of 12.10, 9.48, and 9.87 µM, respectively, without cytotoxicity.
authors.affiliation: Natural Products Laboratory, School of Pharmacy, Sungkyunkwan University.
authors.forename: Ki Hyun
authors.initials: KH
authors.lastname: Kim
authors.forename: Eunjung
authors.initials: E
authors.lastname: Moon
authors.forename: Sang Keun
authors.initials: SK
authors.lastname: Ha
authors.forename: Won Se
authors.initials: WS
authors.lastname: Suh
authors.forename: Ho Kyung
authors.initials: HK
authors.lastname: Kim
authors.forename: Sun Yeou
authors.initials: SY
authors.lastname: Kim
authors.forename: Sang Un
authors.initials: SU
authors.lastname: Choi
authors.forename: Kang Ro
authors.initials: KR
authors.lastname: Lee
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000032:analysis* / Q000302:isolation & purification / Q000494:pharmacology*; D000972:Antineoplastic Agents, Phytogenic / Q000032:analysis* / Q000302:isolation & purification / Q000494:pharmacology*; D002460:Cell Line; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D006801:Humans; D017705:Lignans / Q000032:analysis* / Q000302:isolation & purification / Q000494:pharmacology*; D027443:Lindera / Q000737:chemistry*; D051379:Mice; D017628:Microglia / Q000187:drug effects / Q000276:immunology; D009369:Neoplasms / Q000188:drug therapy; D009569:Nitric Oxide / Q000032:analysis / Q000276:immunology; D010936:Plant Extracts / Q000737:chemistry
chemical_list: D000893:Anti-Inflammatory Agents; D000972:Antineoplastic Agents, Phytogenic; D017705:Lignans; D010936:Plant Extracts; D009569:Nitric Oxide