title: Associations between pig adiponectin (ADIPOQ) genotype and serum lipid levels are modulated by age-specific modifiers.
abstract: The adiponectin (ADIPOQ) locus is a positional and functional candidate gene for 2 porcine chromosome 13 (SSC13) QTL influencing cholesterol (CHOL) and low-density lipoprotein (LDL) concentrations in 190-d-old pigs. By sequencing 2.37 kb of the pig ADIPOQ cDNA, we have identified 1 c.*1512G>T 3' untranslated region polymorphism that has been genotyped in a Duroc pig commercial population with records for serum lipid levels at 45 and 190 d of age. Statistical analysis of the data have revealed significant associations between the ADIPOQ genotype and CHOL (P=0.0040) and LDL (P=0.0011) concentrations at 190 d but not at 45 d. In family 3, most of the SSC13 QTL effects on LDL levels at 190 d were explained by the ADIPOQ genotype. We also found an association with triglyceride levels at 45 d (P=0.0060) but not at 190 d. Measurement of allelic mRNA imbalance demonstrated that the G and T alleles are expressed at very similar levels in muscle and fat tissues, indicating that the c.*1512G>T polymorphism does not affect transcript abundance. As a whole, results obtained in the current work as well as previous data gathered in humans and pigs provide evidence that the magnitude of associations between blood lipid phenotypes and candidate loci genotypes may vary depending on the age of the individual, therefore suggesting the existence of dynamic genotype×environment interactions changing on a temporal scale.
authors.affiliation: Department of Animal Genetics, Center for Research in Agricultural Genomics (CSIC-IRTA-UAB-UB), Campus de la Universitat Autònoma de Barcelona, Bellaterra, 08193 Spain Departament de Ciència Animal i dels Aliments, Facultat de Veterinària, Universitat Autònoma de Barcelona, Bellaterra, 08193 Spain.
authors.forename: A
authors.initials: A
authors.lastname: Castelló
authors.affiliation: IRTA, Genètica i Millora Animal, Torre Marimon, 08140 Caldes de Montbui, Spain.
authors.forename: R
authors.initials: R
authors.lastname: Quintanilla
authors.affiliation: Department of Animal Genetics, Center for Research in Agricultural Genomics (CSIC-IRTA-UAB-UB), Campus de la Universitat Autònoma de Barcelona, Bellaterra, 08193 Spain.
authors.forename: C
authors.initials: C
authors.lastname: Melo
authors.affiliation: Departament de Ciència Animal i dels Aliments, Facultat de Veterinària, Universitat Autònoma de Barcelona, Bellaterra, 08193 Spain.
authors.forename: D
authors.initials: D
authors.lastname: Gallardo
authors.affiliation: Department of Animal Genetics, Center for Research in Agricultural Genomics (CSIC-IRTA-UAB-UB), Campus de la Universitat Autònoma de Barcelona, Bellaterra, 08193 Spain.
authors.forename: A
authors.initials: A
authors.lastname: Zidi
authors.affiliation: Department of Animal Genetics, Center for Research in Agricultural Genomics (CSIC-IRTA-UAB-UB), Campus de la Universitat Autònoma de Barcelona, Bellaterra, 08193 Spain.
authors.forename: A
authors.initials: A
authors.lastname: Manunza
authors.affiliation: IRTA, Genètica i Millora Animal, Torre Marimon, 08140 Caldes de Montbui, Spain.
authors.forename: J L
authors.initials: JL
authors.lastname: Noguera
authors.affiliation: IRTA, Genètica i Millora Animal, Torre Marimon, 08140 Caldes de Montbui, Spain.
authors.forename: J
authors.initials: J
authors.lastname: Tibau
authors.affiliation: Departament de Ciència Animal i dels Aliments, Facultat de Veterinària, Universitat Autònoma de Barcelona, Bellaterra, 08193 Spain.
authors.forename: J
authors.initials: J
authors.lastname: Jordana
authors.affiliation: Department of Animal Production, University of Lleida-Agrotecnio Center, 25198, Lleida, Spain.
authors.forename: R N
authors.initials: RN
authors.lastname: Pena
authors.affiliation: Department of Animal Genetics, Center for Research in Agricultural Genomics (CSIC-IRTA-UAB-UB), Campus de la Universitat Autònoma de Barcelona, Bellaterra, 08193 Spain Departament de Ciència Animal i dels Aliments, Facultat de Veterinària, Universitat Autònoma de Barcelona, Bellaterra, 08193 Spain Marcel.Amills@uab.cat.
authors.forename: M
authors.initials: M
authors.lastname: Amills
mesh_terms: D052242:Adiponectin / Q000235:genetics*; D000367:Age Factors; D000483:Alleles; D000818:Animals; D002784:Cholesterol / Q000097:blood; D005838:Genotype; D006801:Humans; D008055:Lipids / Q000097:blood*; D010641:Phenotype; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D034421:Sus scrofa / Q000097:blood / Q000235:genetics*; D013552:Swine
chemical_list: D052242:Adiponectin; D008055:Lipids; D002784:Cholesterol
keywords: adiponectin; cholesterol; low-density lipoprotein; pig; pyrosequencing; triglyceride