title: p32 is a novel target for viral protein ICP34.5 of herpes simplex virus type 1 and facilitates viral nuclear egress.
abstract: As a large double-stranded DNA virus, herpes simplex virus type 1 (HSV-1) assembles capsids in the nucleus where the viral particles exit by budding through the inner nuclear membrane. Although a number of viral and host proteins are involved, the machinery of viral egress is not well understood. In a search for host interacting proteins of ICP34.5, which is a virulence factor of HSV-1, we identified a cellular protein, p32 (gC1qR/HABP1), by mass spectrophotometer analysis. When expressed, ICP34.5 associated with p32 in mammalian cells. Upon HSV-1 infection, p32 was recruited to the inner nuclear membrane by ICP34.5, which paralleled the phosphorylation and rearrangement of nuclear lamina. Knockdown of p32 in HSV-1-infected cells significantly reduced the production of cell-free viruses, suggesting that p32 is a mediator of HSV-1 nuclear egress. These observations suggest that the interaction between HSV-1 ICP34.5 and p32 leads to the disintegration of nuclear lamina and facilitates the nuclear egress of HSV-1 particles.
authors.affiliation: From the Key laboratory of Microbial Functional Genomics of the Ministry of Education, College of Life Sciences, Nankai University, Tianjin 300071, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Wang
authors.affiliation: From the Key laboratory of Microbial Functional Genomics of the Ministry of Education, College of Life Sciences, Nankai University, Tianjin 300071, China.
authors.forename: Yin
authors.initials: Y
authors.lastname: Yang
authors.affiliation: From the Key laboratory of Microbial Functional Genomics of the Ministry of Education, College of Life Sciences, Nankai University, Tianjin 300071, China.
authors.forename: Songfang
authors.initials: S
authors.lastname: Wu
authors.affiliation: From the Key laboratory of Microbial Functional Genomics of the Ministry of Education, College of Life Sciences, Nankai University, Tianjin 300071, China.
authors.forename: Shuang
authors.initials: S
authors.lastname: Pan
authors.affiliation: Department of Biochemistry, Institute for Drug Control, Tianjin 300070, China.
authors.forename: Chaodong
authors.initials: C
authors.lastname: Zhou
authors.affiliation: Department of Microbiology and Immunology, College of Medicine, University of Illinois, Chicago, Illinois 60612, and.
authors.forename: Yijie
authors.initials: Y
authors.lastname: Ma
authors.affiliation: Department of Electron Microscopy, Institute of Hematology and Blood Diseases Hospital, Peking Union College, Tianjin 300020, China.
authors.forename: Yongxin
authors.initials: Y
authors.lastname: Ru
authors.affiliation: Department of Electron Microscopy, Institute of Hematology and Blood Diseases Hospital, Peking Union College, Tianjin 300020, China.
authors.forename: Shuxu
authors.initials: S
authors.lastname: Dong
authors.affiliation: Department of Microbiology and Immunology, College of Medicine, University of Illinois, Chicago, Illinois 60612, and tshuo@uic.edu.
authors.forename: Bin
authors.initials: B
authors.lastname: He
authors.affiliation: From the Key laboratory of Microbial Functional Genomics of the Ministry of Education, College of Life Sciences, Nankai University, Tianjin 300071, China, czz912@aliyun.com.
authors.forename: Cuizhu
authors.initials: C
authors.lastname: Zhang
authors.affiliation: From the Key laboratory of Microbial Functional Genomics of the Ministry of Education, College of Life Sciences, Nankai University, Tianjin 300071, China, caoyj@nankai.edu.cn.
authors.forename: Youjia
authors.initials: Y
authors.lastname: Cao
mesh_terms: D000818:Animals; D002352:Carrier Proteins / Q000378:metabolism*; D002467:Cell Nucleus / Q000821:virology*; D002522:Chlorocebus aethiops; D057809:HEK293 Cells; D006367:HeLa Cells; D018259:Herpesvirus 1, Human / Q000502:physiology*; D054884:Host-Pathogen Interactions; D006801:Humans; D024101:Mitochondrial Proteins / Q000378:metabolism*; D025941:Protein Interaction Mapping; D021381:Protein Transport; D018160:Receptors, Cytoplasmic and Nuclear / Q000378:metabolism; D014709:Vero Cells; D014764:Viral Proteins / Q000378:metabolism*; D057074:Virus Release; D000097682:Lamin B Receptor
chemical_list: D002352:Carrier Proteins; D024101:Mitochondrial Proteins; D018160:Receptors, Cytoplasmic and Nuclear; D014764:Viral Proteins; D000097682:Lamin B Receptor; C087299:C1QBP protein, human; C066469:gamma 34.5 protein, Human herpesvirus 1
keywords: HSV; Herpesvirus; ICP34.5; Nuclear Lamina; Nuclear Membrane; Protein-Protein Interaction; Viral Egress; Viral Protein; p32 (gC1qR/HABP1)