title: Prognostic biomarkers for HNSCC using quantitative real-time PCR and microarray analysis: β-tubulin isotypes and the p53 interactome.
abstract: In 2014, more than 40,000 people in the United States will be diagnosed with head and neck squamous cell cancer (HNSCC) and nearly 8400 people will die of the disease (www.cancer.org/acs/groups). Little is known regarding molecular targets that might lead to better therapies and improved outcomes for these patients. The incorporation of taxanes into the standard cisplatin/5-fluouracil initial chemotherapy for HNSCC has been associated with improved response rate and survival. Taxanes target the β-subunit of the tubulin heterodimers, the major protein in microtubules, and halt cell division at G2/M phase. Both laboratory and clinical research suggest a link between β-tubulin expression and cancer patient survival, indicating that patterns of expression for β-tubulin isotypes along with activity of tumor suppressors such as p53 or micro-RNAs could be useful prognostic biomarkers and could suggest therapeutic targets. © 2014 Wiley Periodicals, Inc.
authors.affiliation: School of Nursing, University of Mississippi Medical Center, Jackson, Mississippi.
authors.forename: Sharon
authors.initials: S
authors.lastname: Lobert
authors.forename: Mary E
authors.initials: ME
authors.lastname: Graichen
authors.forename: Robert D
authors.initials: RD
authors.lastname: Hamilton
authors.forename: Karen T
authors.initials: KT
authors.lastname: Pitman
authors.forename: Michael R
authors.initials: MR
authors.lastname: Garrett
authors.forename: Chindo
authors.initials: C
authors.lastname: Hicks
authors.forename: Tejaswi
authors.initials: T
authors.lastname: Koganti
mesh_terms: D015415:Biomarkers; D002294:Carcinoma, Squamous Cell / Q000276:immunology*; D006258:Head and Neck Neoplasms / Q000276:immunology*; D006801:Humans; D046228:Microarray Analysis; D011379:Prognosis; D060888:Real-Time Polymerase Chain Reaction; D000077195:Squamous Cell Carcinoma of Head and Neck; D014404:Tubulin / Q000378:metabolism*; D016159:Tumor Suppressor Protein p53 / Q000235:genetics / Q000378:metabolism*
chemical_list: D015415:Biomarkers; D014404:Tubulin; D016159:Tumor Suppressor Protein p53
keywords: HNSCC; TUBB3; p53; tubulin; tubulin isotypes