title: Identification of Acremonium acutatum and Trichothecium roseum isolated from Grape with White Stain Symptom in Korea.
abstract: During 2010 and 2012 grape harvest seasons in Gyeonggi-do, Korea, a white stain symptom was observed on the harvested grape fruits in 'Campbell-Early' and 'Kyoho' varieties. In samples collected from the infected vine, two different strains of pathogenic fungi have been found and identified as Acremonium acutatum and Trichothecium roseum based on fungal morphology and nucleotide sequence of internal transcribed spacer (ITS) and supported by the phylogenetic analysis of the rDNA-ITS region. The DNA homologies of the isolated strains were 99.8% and 99.6% identical with T. roseum (IFB-22133) and A. acutatum (CBS682.71), respectively. In the pathogenicity test, the spores of A. acutatum and T. roseum sprayed on the grapes caused white stain symptoms on the fruits in two weeks after the artificial inoculation, which is similar to observations in the field. To our knowledge, this is the first report of white stain symptoms caused by A. acutatum and T. roseum on the grapes in Korea.
authors.affiliation: Research Institute of International Agriculture, Technology and Information, Hankyong National University, Anseong 456-749, Korea.
authors.forename: Soh-Young
authors.initials: SY
authors.lastname: Oh
authors.affiliation: Research Institute of International Agriculture, Technology and Information, Hankyong National University, Anseong 456-749, Korea. ; Department of Horticulture, Hankyong National University, Anseong 456-749, Korea.
authors.forename: Ki-Woong
authors.initials: KW
authors.lastname: Nam
authors.affiliation: Research Institute of International Agriculture, Technology and Information, Hankyong National University, Anseong 456-749, Korea.
authors.forename: Deok-Hoon
authors.initials: DH
authors.lastname: Yoon
keywords: Acremonium acutatum; Grape; Trichothecium roseum; White stain symptom