title: Preparation, characterization, and optimization of altretamine-loaded solid lipid nanoparticles using Box-Behnken design and response surface methodology.
abstract: The objective of the present study was to prepare solid lipid nanoparticles (SLNs) of altretamine (ALT) by the hot homogenization and ultrasonication method. The study was conducted using the Box-Behnken design (BBD), with a 3(3) design and a total of 17 experimental runs, performed in combination with response surface methodology (RSM). The SLNs were evaluated for mean particle size, entrapment efficiency, and drug-loading. The optimized formulation, with a desirability factor of 0.92, was selected and characterized. In vitro release studies showed a biphasic release pattern from the SLNs for up to 24 h. The results of % EE (93.21 ± 1.5), %DL (1.15 ± 0.6), and mean diameter of (100.6 ± 2.1) nm, were very close to the predicted values.
authors.affiliation: a University Institute of Pharmacy, Pt. Ravi Shankar Shukla University , Raipur , Chhattisgarh , India.
authors.forename: Bina
authors.initials: B
authors.lastname: Gidwani
authors.affiliation: a University Institute of Pharmacy, Pt. Ravi Shankar Shukla University , Raipur , Chhattisgarh , India.
authors.forename: Amber
authors.initials: A
authors.lastname: Vyas
mesh_terms: D006585:Altretamine / Q000737:chemistry*; D004337:Drug Carriers / Q000737:chemistry*; D065546:Drug Liberation; D004355:Drug Stability; D008055:Lipids / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D013501:Surface-Active Agents / Q000737:chemistry; D000069453:Ultrasonic Waves
chemical_list: D004337:Drug Carriers; D008055:Lipids; D013501:Surface-Active Agents; D006585:Altretamine
keywords: Box-Behnken design; altretamine; optimization; response surface methodology; solid lipid nanoparticles