title: Zoonotic Dirofilaria repens (Nematoda: Filarioidea) in Aedes vexans mosquitoes, Czech Republic.
abstract: The surveillance of vectors for arthropod-borne pathogens is nowadays an important tool in surveillance programmes throughout Europe. Whereas many studies have been performed to screen arthropods for viruses or bacterial pathogens, only limited information is available concerning the geographical distribution and vector range of pathogenic filariae in Central Europe. To consider the prevalence of filarial parasites in mosquito vectors, we performed a molecular survey of mosquitoes for filarial DNA. Mosquito collection was conducted at six study sites in the South Moravian region (Czech Republic) close to the borders with Slovakia and Austria from 2009 to 2011. Molecular screening of mosquitoes was conducted using conventional PCR with primers designed to amplify the mitochondrial cytochromoxidase subunit I gene as well as the partial 5.8S ribosomal RNA gene. A total of 13,222 mosquitoes belonging to six species were captured and distributed into 237 pools with different numbers of individuals. Overall, four pools were positive for Dirofilaria repens (a minimum infection rate 0.03%) at two study sites (both natural and urban). Another filarial parasite detected during a study into Aedes vexans mosquitoes revealed the closest homology to Setaria spp. We detected specific D. repens DNA in Ae. vexans mosquitoes for the first time in the Czech Republic and confirmed the circulation of Dirofilaria spp. in a natural focus of infection providing an epidemiological link between autochthonous canine cases and mosquito vectors in the area studied.
authors.affiliation: Academy of Sciences, Institute of Vertebrate Biology, v.v.i., Kvetna 8, 603 65, Brno, Czech Republic, rudolf@ivb.cz.
authors.forename: Ivo
authors.initials: I
authors.lastname: Rudolf
authors.forename: Oldřich
authors.initials: O
authors.lastname: Šebesta
authors.forename: Jan
authors.initials: J
authors.lastname: Mendel
authors.forename: Lenka
authors.initials: L
authors.lastname: Betášová
authors.forename: Eva
authors.initials: E
authors.lastname: Bocková
authors.forename: Petra
authors.initials: P
authors.lastname: Jedličková
authors.forename: Kristýna
authors.initials: K
authors.lastname: Venclíková
authors.forename: Hana
authors.initials: H
authors.lastname: Blažejová
authors.forename: Silvie
authors.initials: S
authors.lastname: Šikutová
authors.forename: Zdeněk
authors.initials: Z
authors.lastname: Hubálek
mesh_terms: D000330:Aedes / Q000145:classification / Q000469:parasitology*; D000818:Animals; D001483:Base Sequence; D018153:Czech Republic / Q000453:epidemiology; D060348:Dirofilaria repens / Q000235:genetics / Q000302:isolation & purification*; D004184:Dirofilariasis / Q000453:epidemiology / Q000469:parasitology / Q000635:transmission*; D004283:Dog Diseases / Q000453:epidemiology / Q000469:parasitology / Q000635:transmission*; D004285:Dogs; D005260:Female; D005618:Fresh Water; D005843:Geography; D006801:Humans; D007303:Insect Vectors / Q000145:classification / Q000469:parasitology*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000662:veterinary; D011634:Public Health; D017422:Sequence Analysis, DNA / Q000662:veterinary; D015047:Zoonoses / Q000469:parasitology / Q000635:transmission