title: Tissue fatty acid composition and secretory phospholipase-A2 activity in oral squamous cell carcinoma.
abstract: OBJECTIVE
  Oral squamous cell carcinoma (OSCC) is a remarkable health problem worldwide, but its pathogenesis remains unknown. The aim of this study was to compare fat composition and secretory phospholipase-A2 (sPLA2) activity between the malignant and adjacent normal squamous tissues in patients with OSCC.
  
  
  METHODS
  Paired samples of malignant squamous and adjacent normal-appearing tissues were collected from 27 patients with OSCC. The fatty acid composition in the obtained tissues was determined by gas liquid chromatography. Tissue enzyme activities of sPLA2 were measured using the standard assay with Diheptanoyl Thio-Phosphatidylcholine as substrate.
  
  
  RESULTS
  In the OSCC tissue, the level of stearic acid (18:0) and activity of sPLA2 were higher (P < 0.001), and the levels of oleic acid (18:1n-9) and linoleic acid (18:2n-6) were lower than that in the adjacent normal-appearing squamous tissue (P < 0.001). The activity of sPLA2 in OSCC was strongly negatively correlated with the amount of 18:2n-6 (r = -0.41, P < 0.001). Negative significant associations were observed between the OSCC invasion and tissue levels of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHE).
  
  
  CONCLUSIONS
  The changes in the fatty acid composition and sPLA2 activity may be regarded as indicators of altered lipid metabolism occurring in vivo during squamous cell carcinogenesis.
authors.affiliation: Department of Maxillofacial Pathology, Faculty of Dentistry, International Campus, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: M
authors.initials: M
authors.lastname: Askari
authors.forename: M
authors.initials: M
authors.lastname: Darabi
authors.forename: R
authors.initials: R
authors.lastname: Zare Mahmudabadi
authors.forename: M
authors.initials: M
authors.lastname: Oboodiat
authors.forename: S
authors.initials: S
authors.lastname: Fayezi
authors.forename: Z
authors.initials: Z
authors.lastname: Mostakhdemin Hosseini
authors.forename: A
authors.initials: A
authors.lastname: Pirzadeh
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D002294:Carcinoma, Squamous Cell / Q000737:chemistry* / Q000201:enzymology / Q000473:pathology; D004281:Docosahexaenoic Acids / Q000032:analysis; D015118:Eicosapentaenoic Acid / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis*; D005260:Female; D006801:Humans; D019787:Linoleic Acid / Q000032:analysis; D008297:Male; D008875:Middle Aged; D009061:Mouth Mucosa / Q000737:chemistry / Q000201:enzymology; D009062:Mouth Neoplasms / Q000737:chemistry* / Q000201:enzymology / Q000473:pathology; D009361:Neoplasm Invasiveness; D019301:Oleic Acid / Q000032:analysis; D009959:Oropharyngeal Neoplasms / Q000737:chemistry* / Q000201:enzymology / Q000473:pathology; D054467:Phospholipases A2 / Q000378:metabolism*; D013229:Stearic Acids / Q000032:analysis
chemical_list: D005227:Fatty Acids; D013229:Stearic Acids; D004281:Docosahexaenoic Acids; D019301:Oleic Acid; C031183:stearic acid; D019787:Linoleic Acid; D015118:Eicosapentaenoic Acid; D054467:Phospholipases A2