title: [Simultaneous determination of sugar alcohols and sugars in functional foods by precolumn ultraviolet derivatization--high performance liquid chromatography].
abstract: OBJECTIVE
  To establish a method using precolumn ultraviolet derivatization coupled with high performance liquid chromatography (HPLC) for simultaneous determination of erythritol, xylitol, galactitol, sorbitol, mannitol, maltitol, glucose and sucrose in functional foods.
  
  
  METHODS
  Target sugar alcohols and sugars in food samples were extracted in water by ultrasonic method and then reacted with benzoyl chloride to form violet-absorbing products, which were separated on a C18 column with gradient elution using methanol and water as mobile phase. The experiment was performed using a flow rate of 1.00 mL/min, column temperature at 30 degrees C and detected wavelength at 232 nm.
  
  
  RESULTS
  The linear correlation coefficients of all the derivatives were more than 0. 999. The detection limits of the method were as low as 2. 2 microg/mL. The average recoveries were 89.6%-117.0%, with intraday relative standard derivations lower than 5%.
  
  
  CONCLUSIONS
  This method is simple, inexpensive and easy to operate and it is suitable for the determination of sugar alcohols and glucose and sucrose in functional foods.
authors.forename: Ya-Pan
authors.initials: YP
authors.lastname: Liu
authors.forename: Xue-Qin
authors.initials: XQ
authors.lastname: Ran
authors.forename: Lu-Ying
authors.initials: LY
authors.lastname: Chen
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.forename: Jia
authors.initials: J
authors.lastname: Ruan
authors.forename: Yong-Xin
authors.initials: YX
authors.lastname: Li
authors.forename: Chengjun
authors.initials: C
authors.lastname: Sun
mesh_terms: D002241:Carbohydrates / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D055951:Functional Food / Q000032:analysis*; D013402:Sugar Alcohols / Q000032:analysis*
chemical_list: D002241:Carbohydrates; D013402:Sugar Alcohols