title: Vitreoscilla hemoglobin promotes Salecan production by Agrobacterium sp. ZX09.
abstract: Salecan is a novel exopolysaccharide produced by the strain Agrobacterium sp. ZX09, and it is composed of only glucose monomers. The unique chemical composition and excellent physicochemical properties make Salecan a promising material for applications in coagulation, lubrication, protection against acute liver injury, and alleviating constipation. In this study, we cloned the Vitreoscilla hemoglobin gene into a broad-host-range plasmid pCM158. Without antibiotic selection, there was negligible loss of the plasmid in the host Agrobacterium sp. ZX09 after one passage of cultivation. The expression of Vitreoscilla hemoglobin was demonstrated by carbon monoxide (CO) difference spectrum. The engineered strain Agrobacterium sp. ZX09 increased Salecan yield by 30%. The other physiological changes included its elevated respiration rate and cellular invertase activity.
authors.affiliation: Center for Molecular Metabolism, Nanjing University of Science & Technology, Nanjing 210094, China.
authors.forename: Yun-mei
authors.initials: YM
authors.lastname: Chen
authors.forename: Hai-yang
authors.initials: HY
authors.lastname: Xu
authors.forename: Yang
authors.initials: Y
authors.lastname: Wang
authors.forename: Jian-fa
authors.initials: JF
authors.lastname: Zhang
authors.forename: Shi-ming
authors.initials: SM
authors.lastname: Wang
mesh_terms: D060054:Agrobacterium / Q000502:physiology*; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism*; D003001:Cloning, Molecular; D024861:Genetic Enhancement / Q000379:methods*; D011994:Recombinant Proteins / Q000378:metabolism; D054793:Truncated Hemoglobins / Q000235:genetics* / Q000378:metabolism*; D047071:beta-Glucans / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D011994:Recombinant Proteins; D054793:Truncated Hemoglobins; D047071:beta-Glucans; C577623:salecan; C082613:hemoglobin protein, Vitreoscilla
keywords: Agrobacterium sp. ZX09; Invertase; Respiration rate; Salecan; Vitreoscilla hemoglobin