title: MicroRNAs expression in triple negative vs non triple negative breast cancer in Tunisia: interaction with clinical outcome.
abstract: BACKGROUND
  MicroRNAs are small, non coding regulatory molecules containing approximately 21 to 25 nucleotides. They function as controllers of expression at post transcriptional levels of most human protein-coding genes and play an essential role in cell signaling pathways. The objective of the present study is to evaluate the expression profile of the following micro-RNAs: miR-10b, miR-17, miR-21, miR-34a, miR-146a, miR-148a and miR-182, and to determine their possible interaction in triple-negative and non triple-negative primary breast cancers based on clinical outcome.
  
  
  METHODS
  60 triple-negative and non triple-negative breast cancer cases, along with their corresponding normal samples were investigated in relation to the expression of the seven studied miRNAs using qPCR Syber Green.
  
  
  RESULTS
  We observed that miR-21, miR-146a and miR-182 were significantly over expressed in triple negative breast cancer. Moreover, miR-10b, miR-21 and miR-182 were significantly associated to lymph node metastases occurrence in triple negative breast carcinoma while only miR-10b was associated with grade III in non triple negative breast cancer cases. Almost all the analyzed microRNAs were strongly associated with patients' genico-obstetric history in non triple negative breast cancer cases except for miR-34a. All the studied microRNAs were strongly correlated with the use of the contraceptive pills in non triple negative breast cancer groups. The additive effect of hormonal factors in triple negative breast cancer cases showed an association with all the studied miRs except for miR-34 and miR-146a.
  
  
  CONCLUSIONS
  The studied microRNAs are strongly influenced by environmental factors especially with hormonal patients' history. Moreover, miR-10b, miR-21 and miR-182 could be defined as biomarkers in breast cancer to predict both lymph node metastases and grade III occurrence.
authors.affiliation: Laboratory of Genetics, Immunology and Human Pathology, Faculty of Sciences of Tunis, University of Tunis El Manar, Tunis, Tunisia.
authors.forename: Imen
authors.initials: I
authors.lastname: Medimegh
authors.affiliation: Laboratory of Genetics, Immunology and Human Pathology, Faculty of Sciences of Tunis, University of Tunis El Manar, Tunis, Tunisia.
authors.forename: Ines
authors.initials: I
authors.lastname: Omrane
authors.affiliation: Laboratory of Genetics and Molecular Diagnostic, Centre Jean Perrin, Clermont-Ferrand, France.
authors.forename: Maud
authors.initials: M
authors.lastname: Privat
authors.affiliation: Laboratory of Genetics and Molecular Diagnostic, Centre Jean Perrin, Clermont-Ferrand, France.
authors.forename: Nancy
authors.initials: N
authors.lastname: Uhrhummer
authors.affiliation: Laboratory of Genetics, Immunology and Human Pathology, Faculty of Sciences of Tunis, University of Tunis El Manar, Tunis, Tunisia.
authors.forename: Hajer
authors.initials: H
authors.lastname: Ayari
authors.affiliation: Laboratory of Genetics, Immunology and Human Pathology, Faculty of Sciences of Tunis, University of Tunis El Manar, Tunis, Tunisia.
authors.forename: Fadoua
authors.initials: F
authors.lastname: Belaiba
authors.affiliation: Salah Azaiez Institute of Carcinology, Tunis, Tunisia.
authors.forename: Farhat
authors.initials: F
authors.lastname: Benayed
authors.affiliation: Salah Azaiez Institute of Carcinology, Tunis, Tunisia.
authors.forename: Khaled
authors.initials: K
authors.lastname: Benromdhan
authors.affiliation: Laboratory of Molecular Screening in the Treatment of Breast Cancer, Immunology and Cancer Research Institute, University of Montreal, Montreal, Canada.
authors.forename: Sylvie
authors.initials: S
authors.lastname: Mader
authors.affiliation: Laboratory of Genetics and Molecular Diagnostic, Centre Jean Perrin, Clermont-Ferrand, France.
authors.forename: Ives-Jean
authors.initials: IJ
authors.lastname: Bignon
authors.affiliation: Laboratory of Genetics, Immunology and Human Pathology, Faculty of Sciences of Tunis, University of Tunis El Manar, Tunis, Tunisia.
authors.forename: Amel Benammar
authors.initials: AB
authors.lastname: Elgaaied
mesh_terms: D000328:Adult; D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism*; D018270:Carcinoma, Ductal, Breast / Q000175:diagnosis / Q000378:metabolism* / Q000628:therapy; D005260:Female; D015870:Gene Expression; D006801:Humans; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D011379:Prognosis; D012372:ROC Curve; D016896:Treatment Outcome; D064726:Triple Negative Breast Neoplasms / Q000175:diagnosis / Q000378:metabolism* / Q000628:therapy; D014416:Tunisia
chemical_list: D014408:Biomarkers, Tumor; D035683:MicroRNAs