title: [AFLP analysis on genetic diversity of Angelica sinensis].
abstract: OBJECTIVE
  To explore the genetic diversity and relationship of different germplasm of Angelica sinensis.
  
  
  METHODS
  Amplified fragment length polymorphism (AFLP) markers were developed to analyze genetic polymorphism in 12 populations 117 samples of Angelica sinensis. The amplified fragments were used for cluster analysis among the different germplasm of Angelica sinensis and to construct the genetic phylogenetic tree with NTSYSpc 2. 11 software.
  
  
  RESULTS
  Eight primer combinations selected from 64 primer combinations were used for amplification and a total of 815 fragments were obtained. Analysis identified 812 polymorphic fragments, accounting for 99.63% of the total detected fragments. Different phenotypes and germplasm of Angelica sinensis could be divided by genetic phylogenetic tree analysis.
  
  
  CONCLUSIONS
  AFLP molecular markers can indicate the significant polymorphism and genetic diversity among germplasm resources of Angelica sinensis. The cultivated purple-stemmed and green-stemmed phenotypes of Angelica sinensis may have different genotypes. The results can provide theoretical evidence for reasonable utilization and breeding new cultivar in molecular level.
authors.forename: Hong-Yi
authors.initials: HY
authors.lastname: Zhang
authors.forename: Wen-Bo
authors.initials: WB
authors.lastname: Liao
mesh_terms: D054458:Amplified Fragment Length Polymorphism Analysis; D029971:Angelica sinensis / Q000145:classification / Q000235:genetics*; D016000:Cluster Analysis; D017931:DNA Primers / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics; D014644:Genetic Variation; D010802:Phylogeny; D018515:Plant Leaves / Q000235:genetics
chemical_list: D017931:DNA Primers; D018744:DNA, Plant