title: Regulation of the ahpC gene encoding alkyl hydroperoxide reductase in Mycobacterium smegmatis.
abstract: The ahpC (MSMEG_4891) gene encodes alkyl hydroperoxide reductase C in Mycobacterium smegmatis mc2155 and its expression is induced under oxidative stress conditions. Two well-defined inverted repeat sequences (IR1 and IR2) were identified in the upstream region of ahpC. Using a crp (cAMP receptor protein: MSMEG_6189) mutant and in vitro DNA-binding assay, it was demonstrated that the IR1 sequence serves as a Crp-binding site and that Crp functions as an activator in the regulation of ahpC expression. The expression level of ahpC was shown to be proportional to intracellular cAMP levels. Intracellular levels of cAMP were increased in M. smegmatis, when it was treated with oxidative stress inducers. The IR2 sequence is very similar to the known consensus sequence of FurA-binding sites and involved in the negative regulation of ahpC expression. Taken together, these results suggest that the induction of ahpC expression under oxidative stress conditions probably results from a combinatory effect of both inactivation of FurA by oxidative stress and activation of Crp in response to increased levels of cAMP.
authors.affiliation: Department of Microbiology, Pusan National University, Busan, Korea.
authors.forename: Ha-Na
authors.initials: HN
authors.lastname: Lee
authors.affiliation: Department of Microbiology, Pusan National University, Busan, Korea.
authors.forename: Na-On
authors.initials: NO
authors.lastname: Lee
authors.affiliation: Department of Microbiology and Institute for Immunology and Immunological Diseases, Yonsei University, Seoul, Korea.
authors.forename: Seung J
authors.initials: SJ
authors.lastname: Han
authors.affiliation: Korea Science Academy of KAIST, Busan, Korea.
authors.forename: In-Jeong
authors.initials: IJ
authors.lastname: Ko
authors.affiliation: Department of Microbiology, Pusan National University, Busan, Korea.
authors.forename: Jeong-Il
authors.initials: JI
authors.lastname: Oh
mesh_terms: D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial; D015971:Gene Expression Regulation, Enzymologic; D020102:Mycobacterium smegmatis / Q000201:enzymology* / Q000235:genetics; D018384:Oxidative Stress; D054464:Peroxiredoxins / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D054464:Peroxiredoxins