title: Protein kinase C regulates the expression of cell wall-related genes in RlmA-dependent and independent manners in Aspergillus nidulans.
abstract: A protein kinase C of Aspergillus nidulans, PkcA, is required for cell wall integrity (CWI) and is considered a major component of the regulating pathway. To investigate whether PkcA regulates the transcription of cell wall-related genes, we constructed strains expressing pkcA(R429A) that encodes an activated form of PkcA. The mRNA levels of most chitin synthase genes and an α-glucan synthase gene, agsB, were increased when pkcA(R429A) expression was induced. These mRNA increases were not observed or were only partially observed, in a deletion mutant of rlmA, an ortholog of RLM1 that encodes a transcription factor in the CWI pathway in Saccharomyces cerevisiae. In addition, in a pkcA temperature-sensitive mutant under heat stress, the mRNA levels of some chitin synthase genes and agsB did not increase. These results suggest that PkcA is involved in CWI maintenance through the transcriptional regulation of cell wall-related genes.
authors.affiliation: a Department of Biotechnology , The University of Tokyo , Tokyo , Japan.
authors.forename: Takuya
authors.initials: T
authors.lastname: Katayama
authors.forename: Akinori
authors.initials: A
authors.lastname: Ohta
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Horiuchi
mesh_terms: D001233:Aspergillus nidulans / Q000166:cytology* / Q000235:genetics* / Q000378:metabolism; D002473:Cell Wall / Q000235:genetics*; D002687:Chitin Synthase / Q000235:genetics; D004789:Enzyme Activation; D005656:Fungal Proteins / Q000378:metabolism*; D015966:Gene Expression Regulation, Fungal; D018869:Heat-Shock Response / Q000235:genetics; D011493:Protein Kinase C / Q000378:metabolism*; D014158:Transcription, Genetic
chemical_list: D005656:Fungal Proteins; D002687:Chitin Synthase; C097994:PKCA protein, Aspergillus niger; D011493:Protein Kinase C
keywords: Aspergillus nidulans; RlmA; cell wall integrity; protein kinase C