title: Nutrients intake is associated with DNA methylation of candidate inflammatory genes in a population of obese subjects.
abstract: The aim of the present study was to evaluate the potential association between dietary nutrients and alterations in DNA methylation in a set of five candidate genes, including CD14, Et-1, iNOS, HERV-w and TNFα, in a population of overweight/obese subjects. We evaluated possible associations between gene methylation and clinical blood parameters, including total cholesterol (TC), low- and high-density lipoprotein cholesterol (LDL-C and HDL-C), triglyceride and homocysteine levels. We employed validated methods to assess anthropometric, clinical and dietary data, as well as pyrosequencing to evaluate DNA methylation of the five candidate genes in 165 overweight/obese subjects. There was no association between body mass index and DNA methylation of the five candidate genes in this group of subjects. Positive associations were observed between TNFα methylation and blood levels of LDL-C (β = 0.447, p = 0.002), TC/HDL-C (β = 0.467, p = 0.001) and LDL-C/HDL-C (β = 0.445, p = 0.002), as well as between HERV-w methylation and dietary intakes of β-carotene (β = 0.088, p = 0.051) and carotenoids (β = 0.083, p = 0.029). TNFα methylation showed negative associations with dietary intakes of cholesterol (β = -0.278, p = 0.048), folic acid (β = -0.339, p = 0.012), β-carotene (β = -0.332, p = 0.045), carotenoids (β = -0.331, p = 0.015) and retinol (β = -0.360, p = 0.008). These results suggest a complex relationship among nutrient intake, oxidative stress and DNA methylation.
authors.affiliation: Center of Molecular and Genetic Epidemiology, Department of Clinical Sciences and Community Health, Università degli Studi di Milano, Milan 20122, Italy. valentina.bollati@unimi.it.
authors.forename: Valentina
authors.initials: V
authors.lastname: Bollati
authors.affiliation: Center of Molecular and Genetic Epidemiology, Department of Clinical Sciences and Community Health, Università degli Studi di Milano, Milan 20122, Italy. chiara.favero@unimi.it.
authors.forename: Chiara
authors.initials: C
authors.lastname: Favero
authors.affiliation: Center of Molecular and Genetic Epidemiology, Department of Clinical Sciences and Community Health, Università degli Studi di Milano, Milan 20122, Italy. benedetta.albetti@unimi.it.
authors.forename: Benedetta
authors.initials: B
authors.lastname: Albetti
authors.affiliation: Center of Molecular and Genetic Epidemiology, Department of Clinical Sciences and Community Health, Università degli Studi di Milano, Milan 20122, Italy. letizia.tarantini@unimi.it.
authors.forename: Letizia
authors.initials: L
authors.lastname: Tarantini
authors.affiliation: Center of Molecular and Genetic Epidemiology, Department of Clinical Sciences and Community Health, Università degli Studi di Milano, Milan 20122, Italy. alice.moroni1@gmail.com.
authors.forename: Alice
authors.initials: A
authors.lastname: Moroni
authors.affiliation: Laboratory of Environmental Epigenetics, Exposure Epidemiology and Risk Program, Harvard School of Public Health, Boston, MA 02115, USA. hmbyun@hsph.harvard.edu.
authors.forename: Hyang-Min
authors.initials: HM
authors.lastname: Byun
authors.affiliation: Center of Molecular and Genetic Epidemiology, Department of Clinical Sciences and Community Health, Università degli Studi di Milano, Milan 20122, Italy. valeria.motta@unimi.it.
authors.forename: Valeria
authors.initials: V
authors.lastname: Motta
authors.affiliation: Worker's Health Protection and Promotion Unit, Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico, Milan 20122, Italy. diana.misaela@gmail.com.
authors.forename: Diana Misaela
authors.initials: DM
authors.lastname: Conti
authors.affiliation: Laboratory of Clinical Chemistry & Microbiology, Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico, Milan 20122, Italy. amedea.tirelli@policlinico.mi.it.
authors.forename: Amedea Silvia
authors.initials: AS
authors.lastname: Tirelli
authors.affiliation: Worker's Health Protection and Promotion Unit, Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico, Milan 20122, Italy. luisella.vigna@policlinico.mi.it.
authors.forename: Luisella
authors.initials: L
authors.lastname: Vigna
authors.affiliation: Center of Molecular and Genetic Epidemiology, Department of Clinical Sciences and Community Health, Università degli Studi di Milano, Milan 20122, Italy. pieralberto.bertazzi@unimi.it.
authors.forename: Pier Alberto
authors.initials: PA
authors.lastname: Bertazzi
authors.affiliation: Center of Molecular and Genetic Epidemiology, Department of Clinical Sciences and Community Health, Università degli Studi di Milano, Milan 20122, Italy. angela.pesatori@unimi.it.
authors.forename: Angela Cecilia
authors.initials: AC
authors.lastname: Pesatori
mesh_terms: D000328:Adult; D000368:Aged; D015992:Body Mass Index; D002338:Carotenoids / Q000097:blood; D002784:Cholesterol / Q000097:blood; D008076:Cholesterol, HDL / Q000097:blood; D019175:DNA Methylation; D004435:Eating / Q000235:genetics; D019332:Endothelin-1 / Q000235:genetics; D002149:Energy Intake / Q000235:genetics; D005260:Female; D005492:Folic Acid / Q000097:blood; D015686:Gene Products, env / Q000235:genetics; D006801:Humans; D007249:Inflammation / Q000235:genetics*; D018950:Lipopolysaccharide Receptors / Q000235:genetics; D008077:Lipoproteins, LDL / Q000097:blood; D008297:Male; D008875:Middle Aged; D052247:Nitric Oxide Synthase Type II / Q000235:genetics; D009752:Nutritional Status / Q000235:genetics*; D009765:Obesity / Q000097:blood / Q000235:genetics* / Q000378:metabolism*; D050177:Overweight / Q000235:genetics; D011257:Pregnancy Proteins / Q000235:genetics; D014280:Triglycerides / Q000097:blood; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics; D014801:Vitamin A / Q000097:blood; D019207:beta Carotene / Q000097:blood
chemical_list: D008076:Cholesterol, HDL; D019332:Endothelin-1; D015686:Gene Products, env; D018950:Lipopolysaccharide Receptors; D008077:Lipoproteins, LDL; D011257:Pregnancy Proteins; D014280:Triglycerides; D014409:Tumor Necrosis Factor-alpha; C405335:syncytin; D019207:beta Carotene; D014801:Vitamin A; D002338:Carotenoids; D005492:Folic Acid; D002784:Cholesterol; C496319:NOS2 protein, human; D052247:Nitric Oxide Synthase Type II