title: Generation of Minicells from an Endotoxin-Free Gram-Positive Strain Corynebacterium glutamicum.
abstract: Drug delivery systems (DDSs) incorporating bacterial minicells have been evaluated as a very powerful tool in view of biocompatibility. However, limited studies have been carried out on these systems, mainly using minicells from Salmonella sp. and Escherichia coli. Thus, we generated a new minicell-producing strain from an endotoxin-free Corynebacterium glutamicum by the inactivation of genes related to cell division. The two knockout strains, ΔparA and Δncgl1366, showed distinct abilities to produce minicells. The resulting minicells were purified via sequential antibiotic treatments and centrifugations, which resulted in reproducible yields.
authors.affiliation: Department of Biological Sciences, College of Natural Sciences, Chonnam National University, Gwangju 500-757, Republic of Korea.
authors.forename: Jin-Young
authors.initials: JY
authors.lastname: Lee
authors.affiliation: Department of Microbiology, Chonnam National University Medical School, Gwangju 500-757, Republic of Korea.
authors.forename: Hyon E
authors.initials: HE
authors.lastname: Choy
authors.affiliation: Department of Food Science and Biotechnology, Kyungsung University, Busan 608-736, Republic of Korea.
authors.forename: Jin-Ho
authors.initials: JH
authors.lastname: Lee
authors.affiliation: Department of Biological Sciences, College of Natural Sciences, Chonnam National University, Gwangju 500-757, Republic of Korea.
authors.forename: Geun-Joong
authors.initials: GJ
authors.lastname: Kim
mesh_terms: D048230:Corynebacterium glutamicum / Q000235:genetics*; D004731:Endotoxins / Q000172:deficiency / Q000235:genetics*; D055786:Gene Knockout Techniques; D023061:Gene Order; D018390:Gene Targeting; D005818:Genetic Engineering; D005822:Genetic Vectors / Q000235:genetics; D059765:Homologous Recombination
chemical_list: D004731:Endotoxins