title: Identification of suitable reference genes for gene expression studies by qRT-PCR in the blister beetle Mylabris cichorii.
abstract: The blister beetle Mylabris cichorii L. (Coleoptera: Meloidae) is a traditional medicinal insect recorded in the Chinese Pharmacopoeia. It synthesizes cantharidin, which kills cancer cells efficiently. Only males produce large amounts of cantharidin. Reference genes are required as endogenous controls for the analysis of differential gene expression in M. cichorii. Our study chose 10 genes as candidate reference genes. The stability of expression of these genes was analyzed by quantitative PCR and determined with two algorithms, geNorm and Normfinder. We recommend UBE3A and RPL22e as suitable reference genes in females and UBE3A, TAF5, and RPL22e in males.
authors.affiliation: Bio-Engineering College of Chongqing University, Key Lab of Genetic Function and Regulation in Chongqing, Chongqing, China Panzhihua University, Panzhihua, China wy1980831@yahoo.com.cn.
authors.forename: Yu
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Bio-Engineering College of Chongqing University, Key Lab of Genetic Function and Regulation in Chongqing, Chongqing, China w-zk@163.com.
authors.forename: Zhong-Kang
authors.initials: ZK
authors.lastname: Wang
authors.affiliation: Bio-Engineering College of Chongqing University, Key Lab of Genetic Function and Regulation in Chongqing, Chongqing, China 793989350@qq.com.
authors.forename: Yi
authors.initials: Y
authors.lastname: Huang
authors.affiliation: Bio-Engineering College of Chongqing University, Key Lab of Genetic Function and Regulation in Chongqing, Chongqing, China 281133403@qq.com.
authors.forename: Yu-Feng
authors.initials: YF
authors.lastname: Liao
authors.affiliation: Bio-Engineering College of Chongqing University, Key Lab of Genetic Function and Regulation in Chongqing, Chongqing, China yinyouping@cqu.edu.cn.
authors.forename: You-Ping
authors.initials: YP
authors.lastname: Yin
mesh_terms: D000818:Animals; D001517:Coleoptera / Q000235:genetics* / Q000378:metabolism; D017931:DNA Primers; D005260:Female; D015870:Gene Expression; D017344:Genes, Insect; D008297:Male; D060888:Real-Time Polymerase Chain Reaction; D012015:Reference Standards; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D017931:DNA Primers
keywords: cantharidin; normalization