title: [Impact of dopamine receptor modulation on reduced anxiety-like behavior in neonatal rats after hypoxic-ischemic brain damage].
abstract: OBJECTIVE
  To observe the long-term changes in anxiety-like behavior and tyrosine hydroxylase (TH) expression in the substantia nigra (SN) after hypoxic-ischemic brain damage (HIBD) in a neonatal rat model and to further explore the relationship between dopamine (DA) level and long-term anxiety-like behavior using the DA receptor (DAR) antagonist.
  
  
  METHODS
  Seven-day-old (P7) neonatal Sprague-Dawley (SD) rats were randomized into normal control, sham-operated, HIBD and HIBD+DAR antagonist groups. HIBD model was prepared by ligating the right common carotid artery and 8% hypoxia exposure. The rats in the sham-operated group were sham-operated and were not subjected to right common carotid artery ligation and hypoxia exposure. The DAR antagonist was injected intraperitoneally before and after inducing HIBD. The same amount of normal saline was given to the other three groups as a control. Anxiety-like behavior was evaluated by elevated plus maze test, and TH expression in the SN was measured by immunohistochemistry on P14, P21, and P28.
  
  
  RESULTS
  On P21 and P28, the time spent in the open arms and the percentage of open arms entries in the HIBD group were significantly increased compared with those in the normal control, sham-operated and HIBD+DAR antagonist groups (P&lt;0.05); in addition, the HIBD+DAR antagonist group showed a significantly longer time spent in the open arms than the normal control group (P&lt;0.05). On P14, P21, and P28, TH expression in the HIBD and HIBD+DAR antagonist groups was significantly lower than that in the normal control and sham-operated groups, and TH level in the HIBD group was significantly lower than that in the HIBD+DAR antagonist group (P&lt;0.05).
  
  
  CONCLUSIONS
  DAR antagonist allows the restoration of anxiety-like behavior and alleviates the damage to dopaminergic neurons in SD rats after HIBD.
authors.affiliation: Department of Pediatrics, Third Xiangya Hospital of Central South University, Changsha 410013, China. heiming_yan@aliyun.com.
authors.forename: Hui-Kang
authors.initials: HK
authors.lastname: Tao
authors.forename: Qin
authors.initials: Q
authors.lastname: Tang
authors.forename: Jin-Jin
authors.initials: JJ
authors.lastname: Dai
authors.forename: Yuan-Yuan
authors.initials: YY
authors.lastname: Li
authors.forename: Ming-Yan
authors.initials: MY
authors.lastname: Hei
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001007:Anxiety / Q000209:etiology* / Q000517:prevention & control; D018492:Dopamine Antagonists / Q000627:therapeutic use; D020925:Hypoxia-Ischemia, Brain / Q000150:complications*; D018782:Maze Learning; D051381:Rats; D017207:Rats, Sprague-Dawley; D011954:Receptors, Dopamine / Q000502:physiology*; D013378:Substantia Nigra / Q000201:enzymology; D014446:Tyrosine 3-Monooxygenase / Q000032:analysis
chemical_list: D018492:Dopamine Antagonists; D011954:Receptors, Dopamine; D014446:Tyrosine 3-Monooxygenase