title: Bioactive surface modification of metal oxides via catechol-bearing modular peptides: multivalent-binding, surface retention, and peptide bioactivity.
abstract: A series of multivalent dendrons containing a bioactive osteogenic growth peptide (OGP) domain and surface-binding catechol domains were obtained through solid phase synthesis, and their binding affinity to hydroxyapatite, TiO2, ZrO2, CeO2, Fe3O4 and gold was characterized using a quartz crystal microbalance with dissipation (QCM-d). Using the distinct difference in binding affinity of the bioconjugate to the metal oxides, TiO2-coated glass slides were selectively patterned with bioactive peptides. Cell culture studies demonstrated the bioavailability of the OGP and that OGP remained on the surface for at least 2 weeks under in vitro cell culture conditions. Bone sialoprotein (BSP) and osteocalcein (OCN) markers were upregulated 3-fold and 60-fold, respectively, relative to controls at 21 days. Similarly, 3-fold more calcium was deposited using the OGP tethered dendron compared to TiO2. These catechol-bearing dendrons provide a fast and efficient method to functionalize a wide range of inorganic materials with bioactive peptides and have the potential to be used in coating orthopaedic implants and fixation devices.
authors.affiliation: Department of Polymer Science, The University of Akron , Akron, Ohio 44325, United States.
authors.forename: Wen
authors.initials: W
authors.lastname: Tang
authors.forename: Gina M
authors.initials: GM
authors.lastname: Policastro
authors.forename: Geng
authors.initials: G
authors.lastname: Hua
authors.forename: Kai
authors.initials: K
authors.lastname: Guo
authors.forename: Jinjun
authors.initials: J
authors.lastname: Zhou
authors.forename: Chrys
authors.initials: C
authors.lastname: Wesdemiotis
authors.forename: Gary L
authors.initials: GL
authors.lastname: Doll
authors.forename: Matthew L
authors.initials: ML
authors.lastname: Becker
mesh_terms: D016475:3T3 Cells; D000327:Adsorption; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D002396:Catechols / Q000737:chemistry*; D002448:Cell Adhesion / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D050091:Dendrimers / Q000737:chemistry; D006657:Histones / Q000737:chemistry* / Q000494:pharmacology*; D006863:Hydrogen-Ion Concentration; D055767:Immobilized Proteins / Q000737:chemistry / Q000494:pharmacology; D036341:Intercellular Signaling Peptides and Proteins / Q000737:chemistry* / Q000494:pharmacology*; D051379:Mice; D008903:Minerals / Q000378:metabolism; D010012:Osteogenesis / Q000187:drug effects; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry*
chemical_list: D002396:Catechols; D050091:Dendrimers; D006657:Histones; D055767:Immobilized Proteins; D036341:Intercellular Signaling Peptides and Proteins; D008903:Minerals; C074533:osteogenic growth peptide; C009495:titanium dioxide; D014025:Titanium; D000469:Alkaline Phosphatase; C034221:catechol