title: Effectiveness of HCV core antigen and RNA quantification in HCV-infected and HCV/HIV-1-coinfected patients.
abstract: BACKGROUND
  The measurement of hepatitis C virus core antigen (HCV-coreAg) has been shown to be an indicator of active HCV infection. The aim of the present study was 1) to investigate the stability and effectiveness of HCV-coreAg and HCV-RNA quantification in HCV infection with or without HIV-1 coinfection, 2) to explore the association between the HCV-coreAg/HCV-RNA (Ag/RNA) ratio and the immune status in chronic HCV/HIV-1-coinfected patients.
  
  
  METHODS
  A longitudinal investigation comprised of 227 HCV-monoinfected (n = 129) and HCV/HIV-1-coinfected (n = 98) patients was initiated in August 2009, and 139 (73 with HCV monoinfection and 66 with HCV/HIV-1 coinfection) were followed up in August 2012. Both HCV core antigen and HCV RNA quantification were determined on this cryopreserved plasma. HCV core antigen and HCV RNA quantification were performed subsequently. In addition, an in vitro experiment investigating the possibility of degradation of HCV components (core antigen and RNA) were conducted.
  
  
  RESULTS
  Significant and stable correlations (p < 0.001) were observed both in chronic HCV-monoinfected and HCV/HIV-1-coinfected patients over the 3-year observation. Coinfected patients with immunocompromised condition had a significantly higher (p < 0.05) Ag/RNA ratios than those patients with immunocompetent condition both at two time points (2009 and 2012). Moreover, the Ag/RNA ratios were negatively correlated with CD4+ T-cell counts (p < 0.001). An in vitro experiment investigated the possibility of the slower degradation of HCV particles under HIV-related immunocompromised condition was conducted and the data demonstrated that the Ag/RNA ratios were significantly higher in HIV-1-positive plasma than in healthy plasma (p = 0.005) in this study.
  
  
  CONCLUSIONS
  Our longitudinal study indicated that the HCV-coreAg presented comparable dynamics over time as HCV RNA in chronic HCV-infected patients. Meanwhile, the HCV-coreAg/HCV-RNA ratio was closely associated with immune status in HCV/HIV-1-coinfected patients.
authors.forename: Lu
authors.initials: L
authors.lastname: Long
authors.forename: Tao
authors.initials: T
authors.lastname: Shen
authors.forename: Jian
authors.initials: J
authors.lastname: Gao
authors.forename: Zhaojun
authors.initials: Z
authors.lastname: Duan
authors.forename: Hua
authors.initials: H
authors.lastname: Liang
authors.forename: Fengmin
authors.initials: F
authors.lastname: Lu
mesh_terms: D000914:Antibodies, Viral / Q000097:blood; D060085:Coinfection / Q000097:blood / Q000175:diagnosis* / Q000821:virology; D015658:HIV Infections / Q000097:blood / Q000175:diagnosis* / Q000821:virology; D015497:HIV-1 / Q000235:genetics* / Q000276:immunology; D016174:Hepacivirus / Q000235:genetics; D018936:Hepatitis C Antigens / Q000097:blood* / Q000276:immunology; D019698:Hepatitis C, Chronic / Q000097:blood / Q000175:diagnosis* / Q000821:virology; D006801:Humans; D008137:Longitudinal Studies; D025202:Molecular Diagnostic Techniques; D012367:RNA, Viral / Q000097:blood / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D019562:Viral Load
chemical_list: D000914:Antibodies, Viral; D018936:Hepatitis C Antigens; D012367:RNA, Viral