title: Magainin II modified polydiacetylene micelles for cancer therapy.
abstract: Polydiacetylene (PDA) micelles have been widely used to deliver anticancer drugs in the treatment of a variety of tumours and for imaging living cells. In this study, we developed an effective strategy to directly conjugate magainin II (MGN-II) to the surface of PDA micelles using a fluorescent dye. These stable and well-defined PDA micelles had high cytotoxicity in cancer cell lines, and were able to reduce the tumour size in mice. The modified PDA micelles improved the anticancer effects of MGN-II in the A549 cell line only at a concentration of 16.0 μg mL(-1) (IC50). In addition, following irradiation with UV light at 254 nm, the PDA micelles gave rise to an energy transfer from the fluorescent dye to the backbone of PDA micelles to enhance the imaging of living cells. Our results demonstrate that modified PDA micelles can not only be used in the treatment of tumors in vitro and in vivo in a simple and directed way, but also offer a new platform for designing functional liposomes to act as anticancer agents.
authors.affiliation: Key Labs for Advanced Materials and Institute of Fine Chemicals, East China University of Science and Technology, Shanghai 200237, China. jcwu@ecust.edu.cn tianhe@ecust.edu.cn.
authors.forename: Danling
authors.initials: D
authors.lastname: Yang
authors.forename: Rongfeng
authors.initials: R
authors.lastname: Zou
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhu
authors.forename: Ben
authors.initials: B
authors.lastname: Liu
authors.forename: Defan
authors.initials: D
authors.lastname: Yao
authors.forename: Juanjuan
authors.initials: J
authors.lastname: Jiang
authors.forename: Junchen
authors.initials: J
authors.lastname: Wu
authors.forename: He
authors.initials: H
authors.lastname: Tian
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000008:administration & dosage / Q000737:chemistry; D017209:Apoptosis / Q000187:drug effects; D045744:Cell Line, Tumor; D004058:Diffusion; D015195:Drug Design; D005456:Fluorescent Dyes / Q000737:chemistry; D054806:Magainins / Q000008:administration & dosage* / Q000737:chemistry; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D008823:Micelles; D008856:Microscopy, Fluorescence / Q000379:methods; D053769:Nanocapsules / Q000008:administration & dosage / Q000737:chemistry* / Q000648:ultrastructure; D009374:Neoplasms, Experimental / Q000188:drug therapy* / Q000473:pathology*; D010316:Particle Size; D000078789:Polyacetylene Polymer; D011108:Polymers / Q000737:chemistry*; D053279:Polyynes / Q000737:chemistry*; D016896:Treatment Outcome; D029867:Xenopus Proteins / Q000008:administration & dosage* / Q000737:chemistry
chemical_list: D000970:Antineoplastic Agents; D005456:Fluorescent Dyes; D054806:Magainins; D008823:Micelles; D053769:Nanocapsules; D000078789:Polyacetylene Polymer; D011108:Polymers; D029867:Xenopus Proteins; C053301:magainin 2 peptide, Xenopus; D053279:Polyynes; C082361:polydiacetylene