title: Sampling of intestinal microbiota and targeted amplification of bacterial 16S rRNA genes for microbial ecologic analysis.
abstract: Dysbiosis of host-associated commensal microbiota is emerging as an important factor in risk and phenotype of immunologic, metabolic, and behavioral diseases. Accurate analysis of microbial composition and functional state in humans or mice requires appropriate collection and pre-processing of biospecimens. Methods to sample luminal and mucosal microbiota from human or mouse intestines and to profile microbial phylogenetic composition using 16S rRNA sequencing are presented here. Data generated using the methods in this unit can be used for downstream quantitative analysis of microbial ecology.
authors.affiliation: Department of Pathology and Laboratory Medicine, David Geffen School of Medicine at the University of California at Los Angeles, Los Angeles, California.
authors.forename: Maomeng
authors.initials: M
authors.lastname: Tong
authors.affiliation: Division of Digestive Diseases, Department of Medicine, David Geffen School of Medicine at the University of California at Los Angeles, Los Angeles, California.
authors.forename: Jonathan P
authors.initials: JP
authors.lastname: Jacobs
authors.affiliation: Department of Pathology and Laboratory Medicine, David Geffen School of Medicine at the University of California at Los Angeles, Los Angeles, California.
authors.forename: Ian H
authors.initials: IH
authors.lastname: McHardy
authors.affiliation: Department of Pathology and Laboratory Medicine, David Geffen School of Medicine at the University of California at Los Angeles, Los Angeles, California.
authors.forename: Jonathan
authors.initials: J
authors.lastname: Braun
mesh_terms: D000818:Animals; D000069196:Gastrointestinal Microbiome / Q000235:genetics* / Q000276:immunology; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods*; D006801:Humans; D051379:Mice; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D012329:RNA, Bacterial / Q000235:genetics* / Q000276:immunology; D012336:RNA, Ribosomal, 16S / Q000235:genetics* / Q000276:immunology
chemical_list: D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S
keywords: 16S rRNA; Illumina sequencing; intestinal microbiota; lavage; mucosal microbiota