title: Effect of Casein Phosphopeptide-amorphous Calcium Phosphate Treatment on Microtensile Bond Strength to Carious Affected Dentin Using Two Adhesive Strategies.
abstract: Background and aims. The aim was to evaluate the effect of casein phosphopeptide-amorphous calcium phosphate (CPP-ACP) on microtensile bond strength (μTBS) to carious affected dentin (CAD) using etch-and-rinse and self-etch adhesive systems. Materials and methods. The occlusal surface of 32 human molars with moderate occlusal caries was removed. Infected dentin was removed until reaching CAD and the teeth were randomly divided into two groups based on the Single Bond (SB) and Clearfil SE Bond (CSE) adhesive systems. Before composite resin bonding, each group was subdivided into three subgroups of ND, CAD and CPP-ACP-treated CAD (CAD-CPP) based on the dentin substrate. After dissecting samples to l-mm-thick cross-sections (each subgroup: n = 13), μTBS was measured at a strain rate of 0.5 mm/min. Data was analyzed using two-way ANOVA, independent samples t-test and post-hoc Tukey tests (α=0.05). Results. Bond strength of both adhesive systems to ND was significantly higher than that to CAD (P <0.001) and CAD/CPP (P < 0.001). There were no significant differences between the μTBS of SB to CAD and CAD-CPP (P > 0.05).μTBS of CSE to CAD-CPP was higher than that to CAD; however, the difference was not significant (P > 0.05). Significant differences were found between SB and CSE systems only with CAD substrate (P < 0.001). Conclusion. Regardless of the adhesive system used, surface treatment of CAD with CPP-ACP did not have a significant effect on bond strength. However, bond strength to CAD was higher with SB rather than with CSE.
authors.affiliation: Dental and Periodontal Research Center, Tabriz Medical Sciences University, Tabriz, Iran ; Assistant Professor, Department of Operative Dentistry, Faculty of Dentistry, Tabriz Medical Sciences University, Tabriz, Iran.
authors.forename: Mahmoud
authors.initials: M
authors.lastname: Bahari
authors.affiliation: Professor, Department of Operative Dentistry, Faculty of Dentistry, Tabriz Medical Sciences University, Tabriz, Iran.
authors.forename: Siavash
authors.initials: S
authors.lastname: Savadi Oskoee
authors.affiliation: Professor, Department of Operative Dentistry, Faculty of Dentistry, Tabriz Medical Sciences University, Tabriz, Iran.
authors.forename: Soodabeh
authors.initials: S
authors.lastname: Kimyai
authors.affiliation: Assistant Professor, Department of Oral Medicine, Faculty of Dentistry, Tabriz Medical Sciences University, Tabriz, Iran.
authors.forename: Firoz
authors.initials: F
authors.lastname: Pouralibaba
authors.affiliation: Assistant Professor, Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Tabriz Medical Sciences University, Tabriz, Iran.
authors.forename: Farrokh
authors.initials: F
authors.lastname: Farhadi
authors.affiliation: Post-graduate Student, Department of Oral and Maxillofacial Radiology, Faculty of Dentistry, Hamadan Medical Sciences University, Hamadan, Iran.
authors.forename: Marouf
authors.initials: M
authors.lastname: Norouzi
keywords: Carious affected dentin; casein phosphopeptide–amorphous calcium phosphate; etch-and-rinse; microtensile bond strength; self-etch