title: Urinary bile acids as biomarkers for liver diseases II. Signature profiles in patients.
abstract: Hepatobiliary diseases result in the accumulation of bile acids (BAs) in the liver, systemic blood, and other tissues leading to an unfavorable prognosis. The BA profile was characterized by the calculation of indices that describe the composition, sulfation, and amidation of total and individual BAs. Comparison of the urinary BA profiles between healthy subjects and patients with hepatobiliary diseases demonstrated significantly higher absolute concentrations of individual and total BAs in patients. The percentage sulfation of some individual BAs were different between the two groups. The percentage amidation of overall and most individual BAs was higher in patients than controls. The percentage of primary BAs (CDCA and CA) was higher in patients, whereas the percentage of secondary BAs (DCA and LCA) was lower in patients. BA indices belonging to percentage amidation and percentage composition were better associated with the severity of the liver disease as determined by the model for end-stage liver disease (MELD) score and disease compensation status compared with the absolute concentrations of individual and total BAs. In addition, BA indices corresponding to percentage amidation and percentage composition of certain BAs demonstrated the highest area under the receiver operating characteristic (ROC) curve suggesting their utility as diagnostic biomarkers in clinic. Furthermore, significant increase in the risk of having liver diseases was associated with changes in BA indices.
authors.affiliation: *Department of Pharmaceutical Sciences, College of Pharmacy, Department of Internal Medicine, College of Medicine and Department of Biostatistics, College of Public Health, University of Nebraska Medical Center, Omaha, Nebraska 68198 yalnouti@unmc.edu.
authors.forename: Sai Praneeth R
authors.initials: SP
authors.lastname: Bathena
authors.affiliation: *Department of Pharmaceutical Sciences, College of Pharmacy, Department of Internal Medicine, College of Medicine and Department of Biostatistics, College of Public Health, University of Nebraska Medical Center, Omaha, Nebraska 68198 yalnouti@unmc.edu.
authors.forename: Rhishikesh
authors.initials: R
authors.lastname: Thakare
authors.affiliation: *Department of Pharmaceutical Sciences, College of Pharmacy, Department of Internal Medicine, College of Medicine and Department of Biostatistics, College of Public Health, University of Nebraska Medical Center, Omaha, Nebraska 68198 yalnouti@unmc.edu.
authors.forename: Nagsen
authors.initials: N
authors.lastname: Gautam
authors.affiliation: *Department of Pharmaceutical Sciences, College of Pharmacy, Department of Internal Medicine, College of Medicine and Department of Biostatistics, College of Public Health, University of Nebraska Medical Center, Omaha, Nebraska 68198.
authors.forename: Sandeep
authors.initials: S
authors.lastname: Mukherjee
authors.affiliation: *Department of Pharmaceutical Sciences, College of Pharmacy, Department of Internal Medicine, College of Medicine and Department of Biostatistics, College of Public Health, University of Nebraska Medical Center, Omaha, Nebraska 68198.
authors.forename: Marco
authors.initials: M
authors.lastname: Olivera
authors.affiliation: *Department of Pharmaceutical Sciences, College of Pharmacy, Department of Internal Medicine, College of Medicine and Department of Biostatistics, College of Public Health, University of Nebraska Medical Center, Omaha, Nebraska 68198.
authors.forename: Jane
authors.initials: J
authors.lastname: Meza
authors.affiliation: *Department of Pharmaceutical Sciences, College of Pharmacy, Department of Internal Medicine, College of Medicine and Department of Biostatistics, College of Public Health, University of Nebraska Medical Center, Omaha, Nebraska 68198 yalnouti@unmc.edu.
authors.forename: Yazen
authors.initials: Y
authors.lastname: Alnouti
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000577:Amides / Q000652:urine*; D001647:Bile Acids and Salts / Q000737:chemistry / Q000652:urine*; D001660:Biliary Tract Diseases / Q000652:urine*; D015415:Biomarkers / Q000652:urine; D016022:Case-Control Studies; D003627:Data Interpretation, Statistical; D005260:Female; D064368:Healthy Volunteers; D006801:Humans; D008107:Liver Diseases / Q000652:urine*; D016015:Logistic Models; D008297:Male; D008875:Middle Aged; D013431:Sulfates / Q000652:urine*; D055815:Young Adult
chemical_list: D000577:Amides; D001647:Bile Acids and Salts; D015415:Biomarkers; D013431:Sulfates
keywords: bile acid amidation; bile acid sulfation; bile acids; biomarker; hepatobiliary diseases