title: Membrane-bound mucins and mucin terminal glycans expression in idiopathic or Helicobacter pylori, NSAID associated peptic ulcers.
abstract: OBJECTIVE
  To determine the expression of membrane-bound mucins and glycan side chain sialic acids in Helicobacter pylori (H. pylori)-associated, non-steroidal inflammatory drug (NSAID)-associated and idiopathic-gastric ulcers.
  
  
  METHODS
  We studied a cohort of randomly selected patients with H. pylori (group 1, n = 30), NSAID (group 2, n = 18), combined H. pylori and NSAID associated gastric ulcers (group 3, n = 24), and patients with idiopathic gastric ulcers (group 4, n = 20). Immunohistochemistry for MUC1, MUC4, MUC17, and staining for Erythrina cristagalli agglutinin and Sambucus nigra agglutinin (SNA) lectins was performed on sections from the ulcer margins.
  
  
  RESULTS
  Staining intensity of MUC17 was higher in H. pylori ulcers (group 1) than in idiopathic ulcers (group 4), 11.05 ± 3.67 vs 6.93 ± 4.00 for foveola cells, and 10.29 ± 4.67 vs 8.00 ± 3.48 for gland cells, respectively (P < 0.0001). In contrast, MUC1 expression was higher in group 4 compared group 1, 9.89 ± 4.17 vs 2.93 ± 5.13 in foveola cells and 7.63 ± 4.60 vs 2.57± 4.50 for glands, respectively (P < 0.0001). SNA lectin staining was increased in group 4, in parallel to elevated MUC1 expression, indicating more abundant α2-6 sialylation in that group.
  
  
  CONCLUSIONS
  Cytoplasmic MUC17 staining was significantly decreased in the cases with idiopathic ulcer. The opposite was observed for both MUC1 and SNA lectin. This observation may reflect important pathogenic mechanisms, since different mucins with altered sialylation patterns may differ in their protection efficiency against acid and pepsin.
authors.affiliation: Yaron Niv, Doron Boltin, Zohar Levi, Alex Vilkin, Department of Gastroenterology, Rabin Medical Center, Tel Aviv University, Beilinson Hospital, Tel Aviv 49100, Israel.
authors.forename: Yaron
authors.initials: Y
authors.lastname: Niv
authors.affiliation: Yaron Niv, Doron Boltin, Zohar Levi, Alex Vilkin, Department of Gastroenterology, Rabin Medical Center, Tel Aviv University, Beilinson Hospital, Tel Aviv 49100, Israel.
authors.forename: Doron
authors.initials: D
authors.lastname: Boltin
authors.affiliation: Yaron Niv, Doron Boltin, Zohar Levi, Alex Vilkin, Department of Gastroenterology, Rabin Medical Center, Tel Aviv University, Beilinson Hospital, Tel Aviv 49100, Israel.
authors.forename: Marisa
authors.initials: M
authors.lastname: Halpern
authors.affiliation: Yaron Niv, Doron Boltin, Zohar Levi, Alex Vilkin, Department of Gastroenterology, Rabin Medical Center, Tel Aviv University, Beilinson Hospital, Tel Aviv 49100, Israel.
authors.forename: Miriam
authors.initials: M
authors.lastname: Cohen
authors.affiliation: Yaron Niv, Doron Boltin, Zohar Levi, Alex Vilkin, Department of Gastroenterology, Rabin Medical Center, Tel Aviv University, Beilinson Hospital, Tel Aviv 49100, Israel.
authors.forename: Zohar
authors.initials: Z
authors.lastname: Levi
authors.affiliation: Yaron Niv, Doron Boltin, Zohar Levi, Alex Vilkin, Department of Gastroenterology, Rabin Medical Center, Tel Aviv University, Beilinson Hospital, Tel Aviv 49100, Israel.
authors.forename: Alex
authors.initials: A
authors.lastname: Vilkin
authors.affiliation: Yaron Niv, Doron Boltin, Zohar Levi, Alex Vilkin, Department of Gastroenterology, Rabin Medical Center, Tel Aviv University, Beilinson Hospital, Tel Aviv 49100, Israel.
authors.forename: Sara
authors.initials: S
authors.lastname: Morgenstern
authors.affiliation: Yaron Niv, Doron Boltin, Zohar Levi, Alex Vilkin, Department of Gastroenterology, Rabin Medical Center, Tel Aviv University, Beilinson Hospital, Tel Aviv 49100, Israel.
authors.forename: Vahig
authors.initials: V
authors.lastname: Manugian
authors.affiliation: Yaron Niv, Doron Boltin, Zohar Levi, Alex Vilkin, Department of Gastroenterology, Rabin Medical Center, Tel Aviv University, Beilinson Hospital, Tel Aviv 49100, Israel.
authors.forename: Erica
authors.initials: E
authors.lastname: St Lawrence
authors.affiliation: Yaron Niv, Doron Boltin, Zohar Levi, Alex Vilkin, Department of Gastroenterology, Rabin Medical Center, Tel Aviv University, Beilinson Hospital, Tel Aviv 49100, Israel.
authors.forename: Pascal
authors.initials: P
authors.lastname: Gagneux
authors.affiliation: Yaron Niv, Doron Boltin, Zohar Levi, Alex Vilkin, Department of Gastroenterology, Rabin Medical Center, Tel Aviv University, Beilinson Hospital, Tel Aviv 49100, Israel.
authors.forename: Sukhwinder
authors.initials: S
authors.lastname: Kaur
authors.affiliation: Yaron Niv, Doron Boltin, Zohar Levi, Alex Vilkin, Department of Gastroenterology, Rabin Medical Center, Tel Aviv University, Beilinson Hospital, Tel Aviv 49100, Israel.
authors.forename: Poonam
authors.initials: P
authors.lastname: Sharma
authors.affiliation: Yaron Niv, Doron Boltin, Zohar Levi, Alex Vilkin, Department of Gastroenterology, Rabin Medical Center, Tel Aviv University, Beilinson Hospital, Tel Aviv 49100, Israel.
authors.forename: Surinder K
authors.initials: SK
authors.lastname: Batra
authors.affiliation: Yaron Niv, Doron Boltin, Zohar Levi, Alex Vilkin, Department of Gastroenterology, Rabin Medical Center, Tel Aviv University, Beilinson Hospital, Tel Aviv 49100, Israel.
authors.forename: Samuel B
authors.initials: SB
authors.lastname: Ho
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000009:adverse effects*; D001706:Biopsy; D002462:Cell Membrane / Q000737:chemistry* / Q000187:drug effects / Q000382:microbiology; D005260:Female; D016481:Helicobacter Infections / Q000150:complications / Q000175:diagnosis / Q000382:microbiology*; D016480:Helicobacter pylori / Q000302:isolation & purification*; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D018396:Mucin-1 / Q000032:analysis; D055264:Mucin-4 / Q000032:analysis; D009077:Mucins / Q000032:analysis*; D019158:N-Acetylneuraminic Acid / Q000032:analysis; D037121:Plant Lectins; D011134:Polysaccharides / Q000032:analysis*; D011237:Predictive Value of Tests; D012189:Retrospective Studies; D054788:Ribosome Inactivating Proteins; D013270:Stomach / Q000737:chemistry* / Q000187:drug effects / Q000382:microbiology / Q000473:pathology; D013276:Stomach Ulcer / Q000139:chemically induced / Q000175:diagnosis / Q000378:metabolism* / Q000382:microbiology; D055815:Young Adult
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; C060192:MUC1 protein, human; C450720:MUC17 protein, human; C524658:MUC4 protein, human; D018396:Mucin-1; D055264:Mucin-4; D009077:Mucins; D037121:Plant Lectins; D011134:Polysaccharides; C050600:Sambucus nigra lectins; C045629:erythrina lectin; D054788:Ribosome Inactivating Proteins; D019158:N-Acetylneuraminic Acid
keywords: Glycosylation; Helicobacter pylori; Idiopathic ulcer; Mucin; Mucosal cytoprotection; Peptic ulcer disease