title: Molecular cytogenetic analysis of the crucian carp, Carassius carassius (Linnaeus, 1758) (Teleostei, Cyprinidae), using chromosome staining and fluorescence in situ hybridisation with rDNA probes.
abstract: The crucian carp Carassius carassius (Linnaeus, 1758) is a species with restricted and decreasing distribution in Europe. Six males and six females of the species from the Baltic Sea basin in Poland were examined to show sequentially CMA3/AgNO3 staining pattern, DAPI staining, and, for the first time in literature, molecular cytogenetic analysis using double-colour fluorescence in situ hybridisation (FISH) with 28S and 5S rDNA probes. The karyotype consisted of 20 m, 36 sm and 44 sta chromosomes, NF=156. The AgNO3 stained NORs were most frequently located terminally in the short arms of two sm and two sta elements, and CMA3-positive sites were also observed suggesting abundant GC-rich repetitive DNA in the regions. Other CMA3-positive sites in the short arms of six to ten sm and sta chromosomes were detected. The results based on 28S rDNA FISH confirmed the location of rDNA sites. DAPI-negative staining of NORs suggested the scarcity of AT-rich DNA in the regions. FISH with 5S rDNA probe revealed 8-14 loci (ten and 12 in respectively 49 and 29% of metaphases). They were located in two sm and eight to ten sta chromosomes and six of them were larger than others. Simultaneously, mapping of the two rDNA families on the chromosomes of C. carassius revealed that both 28S and 5S rDNA probes were located in different chromosomes. Molecular cytogenetic data of C. carassius presented here for the first time give an important insight into the structure of chromosomes of this polyploid and declining species and may be useful in its systematics.
authors.affiliation: Department of Zoology, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, M. Oczapowskiego Str. 5, 10-718 Olsztyn, Poland.
authors.forename: Aneta
authors.initials: A
authors.lastname: Spoz
authors.affiliation: Department of Zoology, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, M. Oczapowskiego Str. 5, 10-718 Olsztyn, Poland.
authors.forename: Alicja
authors.initials: A
authors.lastname: Boron
authors.affiliation: Department of Zoology, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, M. Oczapowskiego Str. 5, 10-718 Olsztyn, Poland.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Porycka
authors.affiliation: Department of Zoology, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, M. Oczapowskiego Str. 5, 10-718 Olsztyn, Poland.
authors.forename: Monika
authors.initials: M
authors.lastname: Karolewska
authors.affiliation: Instituto Gulbenkian de Ciência, Rua da Quinta Grade, 6, 2780-156, Oeiras, Portugal.
authors.forename: Daisuke
authors.initials: D
authors.lastname: Ito
authors.affiliation: Sanriku Fisheries Research Center, Department of Revitalization for Sanriku-region, Iwate University, 3-75-1, Heita, Kamaishi 026-0001, Iwate, Japan.
authors.forename: Syuiti
authors.initials: S
authors.lastname: Abe
authors.affiliation: Department of Zoology, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, M. Oczapowskiego Str. 5, 10-718 Olsztyn, Poland.
authors.forename: Lech
authors.initials: L
authors.lastname: Kirtiklis
authors.affiliation: Department of Zoology, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, M. Oczapowskiego Str. 5, 10-718 Olsztyn, Poland.
authors.forename: Dorota
authors.initials: D
authors.lastname: Juchno
keywords: CMA3; Cyprinidae; FISH with rDNA; NOR-phenotype; molecular cytogenetics; polyploid species