title: Magnolol affects expression of IGF-1 and associated binding proteins in human prostate cancer cells in vitro.
abstract: OBJECTIVE
  This study investigated the effects of magnolol, a compound from Magnolia officinalis, on the behavior of LNCaP and PC3 human prostate cancer cells in vitro.
  
  
  METHODS
  In vitro cell culture approach with biochemical tests and Western blot analyses was used.
  
  
  RESULTS
  Magnolol, (80 μM, 6 hour exposure) was found to affect the expression of insulin-like growth factor-1 (IGF-1) and associated proteins. In both cell lines, protein expression of IGF-1 and insulin-like growth factor binding protein-5 (IGFBP-5) were significantly decreased, while protein expression of IGFBP-3 was significantly increased. Additionally, protein expression of insulin-like growth factor-1 receptor (IGF-1R) was significantly increased and the phosphorylated form of IGF-1 (p-IGF-1R) was significantly decreased in PC3 cells, while IGFBP-4 protein expression was significantly increased in LNCaP cells.
  
  
  CONCLUSIONS
  This study has demonstrated for the first time that magnolol can alter the expression of IGF-1 and associated proteins in human prostate cancer cells in vitro and suggests that magnolol may have a potential role as a novel anti-prostate cancer agent.
authors.affiliation: Department of Biology, University of Prince Edward Island, Charlottetown, PE, Canada.
authors.forename: Brendan T
authors.initials: BT
authors.lastname: McKeown
authors.affiliation: Department of Biology, University of Prince Edward Island, Charlottetown, PE, Canada rhurta@upei.ca.
authors.forename: Robert A R
authors.initials: RA
authors.lastname: Hurta
mesh_terms: D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D001713:Biphenyl Compounds / Q000494:pharmacology*; D015153:Blotting, Western; D006801:Humans; D066298:In Vitro Techniques; D018969:Insulin-Like Growth Factor Binding Proteins / Q000378:metabolism*; D007334:Insulin-Like Growth Factor I / Q000378:metabolism*; D017705:Lignans / Q000494:pharmacology*; D008297:Male; D011471:Prostatic Neoplasms / Q000188:drug therapy* / Q000378:metabolism*; D017526:Receptor, IGF Type 1 / Q000378:metabolism*; D014407:Tumor Cells, Cultured
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D001713:Biphenyl Compounds; D018969:Insulin-Like Growth Factor Binding Proteins; D017705:Lignans; C005498:magnolol; D007334:Insulin-Like Growth Factor I; D017526:Receptor, IGF Type 1
keywords: IGF; IGF-BPs; magnolol; prostate cancer cells