title: Association mapping and validation of QTLs for flour yield in the soft winter wheat variety Kitahonami.
abstract: The winter wheat variety Kitahonami shows a superior flour yield in comparison to other Japanese soft wheat varieties. To map the quantitative trait loci (QTL) associated with this trait, association mapping was performed using a panel of lines from Kitahonami's pedigree, along with leading Japanese varieties and advanced breeding lines. Using a mixed linear model corrected for kernel types and familial relatedness, 62 marker-trait associations for flour yield were identified and classified into 21 QTLs. In eighteen of these, Kitahonami alleles showed positive effects. Pedigree analysis demonstrated that a continuous pyramiding of QTLs had occurred throughout the breeding history of Kitahonami. Linkage analyses using three sets of doubled haploid populations from crosses in which Kitahonami was used as a parent were performed, leading to the validation of five of the eight QTLs tested. Among these, QTLs on chromosomes 3B and 7A showed highly significant and consistent effects across the three populations. This study shows that pedigree-based association mapping using breeding materials can be a useful method for QTL identification at the early stages of breeding programs.
authors.affiliation: NARO Tohoku Agricultural Research Center, Morioka, Iwate, Japan.
authors.forename: Goro
authors.initials: G
authors.lastname: Ishikawa
authors.affiliation: NARO Tohoku Agricultural Research Center, Morioka, Iwate, Japan; NARO Kyusyu Okinawa Agricultural Research Center, Chikugo, Fukuoka, Japan.
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Nakamura
authors.affiliation: NARO Tohoku Agricultural Research Center, Morioka, Iwate, Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Ito
authors.affiliation: NARO Tohoku Agricultural Research Center, Morioka, Iwate, Japan.
authors.forename: Mika
authors.initials: M
authors.lastname: Saito
authors.affiliation: Kitami Agricultural Experiment Station, Hokkaido Research Organization, Tokoro-gun, Hokkaido, Japan; Central Agricultural Experiment Station, Hokkaido Research Organization, Yubari-gun, Hokkaido, Japan.
authors.forename: Mikako
authors.initials: M
authors.lastname: Sato
authors.affiliation: Kitami Agricultural Experiment Station, Hokkaido Research Organization, Tokoro-gun, Hokkaido, Japan.
authors.forename: Hironobu
authors.initials: H
authors.lastname: Jinno
authors.affiliation: Kitami Agricultural Experiment Station, Hokkaido Research Organization, Tokoro-gun, Hokkaido, Japan.
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Yoshimura
authors.affiliation: Kitami Agricultural Experiment Station, Hokkaido Research Organization, Tokoro-gun, Hokkaido, Japan; Kamikawa Agricultural Experiment Station, Hokkaido Research Organization, Kamikawa-gun, Hokkaido, Japan.
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Nishimura
authors.affiliation: Nagano Agricultural Experiment Station, Suzaka, Nagano, Japan.
authors.forename: Hidekazu
authors.initials: H
authors.lastname: Maejima
authors.affiliation: Nagano Agricultural Experiment Station, Suzaka, Nagano, Japan.
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Uehara
authors.affiliation: National Institute of Agrobiological Sciences, Kannondai, Tsukuba, Japan.
authors.forename: Fuminori
authors.initials: F
authors.lastname: Kobayashi
authors.affiliation: NARO Tohoku Agricultural Research Center, Morioka, Iwate, Japan.
authors.forename: Toshiki
authors.initials: T
authors.lastname: Nakamura
mesh_terms: D002874:Chromosome Mapping; D005433:Flour; D005819:Genetic Markers / Q000235:genetics; D014644:Genetic Variation; D008957:Models, Genetic; D016133:Polymerase Chain Reaction; D040641:Quantitative Trait Loci; D015203:Reproducibility of Results; D014908:Triticum / Q000737:chemistry* / Q000235:genetics*
chemical_list: D005819:Genetic Markers