title: Optical projection tomography as a tool for 3D imaging of hydrogels.
abstract: An Optical Projection Tomography (OPT) system was developed and optimized to image 3D tissue engineered products based in hydrogels. We develop pre-reconstruction algorithms to get the best result from the reconstruction procedure, which include correction of the illumination and determination of sample center of rotation (CoR). Existing methods for CoR determination based on the detection of the maximum variance of reconstructed slices failed, so we develop a new CoR search method based in the detection of the variance sharpest local maximum. We show the capabilities of the system to give quantitative information of different types of hydrogels that may be useful in its characterization.
authors.affiliation: Tampere University of Technology, ELT, BioMediTech, Tampere, Finland.
authors.forename: Edite
authors.initials: E
authors.lastname: Figueiras
authors.affiliation: Tampere University of Technology, ELT, BioMediTech, Tampere, Finland.
authors.forename: Ana M
authors.initials: AM
authors.lastname: Soto
authors.affiliation: Tampere University of Technology, ELT, BioMediTech, Tampere, Finland.
authors.forename: Danilo
authors.initials: D
authors.lastname: Jesus
authors.affiliation: Tampere University of Technology, ELT, BioMediTech, Tampere, Finland.
authors.forename: M
authors.initials: M
authors.lastname: Lehti
authors.affiliation: Tampere University of Technology, ELT, BioMediTech, Tampere, Finland ; University of Tampere, BioMediTech, Tampere, Finland.
authors.forename: J
authors.initials: J
authors.lastname: Koivisto
authors.affiliation: Tampere University of Technology, ELT, BioMediTech, Tampere, Finland.
authors.forename: J E
authors.initials: JE
authors.lastname: Parraga
authors.affiliation: 3Bs- Research Group, Biomaterials, Biodegradables and Biomimetics, University of Minho, Guimarães, Portugal ; ICVS/3B's - PT Government Associate Laboratory, Braga/Guimarães, Portugal.
authors.forename: J
authors.initials: J
authors.lastname: Silva-Correia
authors.affiliation: 3Bs- Research Group, Biomaterials, Biodegradables and Biomimetics, University of Minho, Guimarães, Portugal ; ICVS/3B's - PT Government Associate Laboratory, Braga/Guimarães, Portugal.
authors.forename: J M
authors.initials: JM
authors.lastname: Oliveira
authors.affiliation: 3Bs- Research Group, Biomaterials, Biodegradables and Biomimetics, University of Minho, Guimarães, Portugal ; ICVS/3B's - PT Government Associate Laboratory, Braga/Guimarães, Portugal.
authors.forename: R L
authors.initials: RL
authors.lastname: Reis
authors.affiliation: Tampere University of Technology, ELT, BioMediTech, Tampere, Finland.
authors.forename: M
authors.initials: M
authors.lastname: Kellomäki
authors.affiliation: Tampere University of Technology, ELT, BioMediTech, Tampere, Finland.
authors.forename: J
authors.initials: J
authors.lastname: Hyttinen
keywords: (100.6890) Three-dimensional image processing; (100.6950) Tomographic image processing; (160.1435) Biomaterials; (170.3010) Image reconstruction techniques