title: An essential role of a ferritin-like protein in acid stress tolerance of Listeria monocytogenes.
abstract: The expression of ten genes of Listeria monocytogenes previously identified as penicillin G-inducible was transcriptionally analyzed in the presence of 0.5 M KCl, pH 5.0 and 42 °C. This study revealed that all the genes are upregulated by osmotic stress, seven by acid stress and four by temperature stress conditions. The contribution of a gene encoding a ferritin-like protein (fri), a two-component phosphate-response regulator (phoP) and an AraC/XylS family transcription regulator (axyR) to temperature, acid and osmotic stress tolerance was further examined by analysis of nonpolar deletion mutants. This revealed that a lack of PhoP or AxyR does not affect the ability to grow under the tested stress conditions. However, the Δ fri strain showed slightly delayed growth under osmotic and clearly impaired growth under acid stress conditions, indicating an important role of the ferritin-like protein in acid stress tolerance.
authors.affiliation: Department of Applied Microbiology, Faculty of Biology, University of Warsaw, Miecznikowa 1, 02-096, Warsaw, Poland.
authors.forename: Dorota
authors.initials: D
authors.lastname: Milecka
authors.forename: Anna
authors.initials: A
authors.lastname: Samluk
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Wasiak
authors.forename: Agata
authors.initials: A
authors.lastname: Krawczyk-Balska
mesh_terms: D000143:Acids / Q000378:metabolism; D000222:Adaptation, Physiological / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D005293:Ferritins / Q000235:genetics / Q000378:metabolism*; D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial; D008089:Listeria monocytogenes / Q000235:genetics / Q000254:growth & development / Q000502:physiology*; D009997:Osmotic Pressure / Q000502:physiology; D017384:Sequence Deletion / Q000235:genetics; D013312:Stress, Physiological / Q000502:physiology; D013696:Temperature
chemical_list: D000143:Acids; D001426:Bacterial Proteins; C418864:ferritin-like protein, Listeria; D005293:Ferritins