title: The profile of Porphyromonas gingivalis kgp biotype and fimA genotype mosaic in subgingival plaque samples.
abstract: Combined analysis of allelic variation of the virulence-associated, strain-specific lys-gingipain gene (kgp) and major fimbrial gene (fimA) of Porphyromonas gingivalis was undertaken in 116 subgingival plaque samples to understand the kgp biotype and fimA genotype profile in a subject-specific manner. Allelic variation in the polyadhesin domain of kgp from P. gingivalis strains 381 (ATCC 33277), HG66 and W83 generated four isoforms corresponding to four biotypes of P. gingivalis. Similarly, variation in the fimA subunit of the fimA gene cluster of P. gingivalis resulted in six fimA genotypes. Strain-specific differential PCR was performed for kgp and fimA using DNA isolated from subgingival plaque samples. Our findings demonstrate that all of the P. gingivalis kgp biotypes detected in this study were predominantly associated with the fimA II genotype. Dominance of kgp biotypes 381 or HG66 combined with fimA II fimbriae could imply an adaptive strategy by P. gingivalis to generate the fittest strains for survival in the host environment.
authors.affiliation: Institute of Dental Research, Westmead Millennium Institute and Westmead Centre for Oral Health, Westmead, NSW, Australia; Faculty of Dentistry, University of Sydney, Sydney, NSW, Australia.
authors.forename: Mangala A
authors.initials: MA
authors.lastname: Nadkarni
authors.forename: Kim-Ly
authors.initials: KL
authors.lastname: Chhour
authors.forename: Cheryl C
authors.initials: CC
authors.lastname: Chapple
authors.forename: Ky-Anh
authors.initials: KA
authors.lastname: Nguyen
authors.forename: Neil
authors.initials: N
authors.lastname: Hunter
mesh_terms: D018829:Adhesins, Bacterial / Q000235:genetics*; D000328:Adult; D001426:Bacterial Proteins / Q000235:genetics; D016866:Bacteroidaceae Infections / Q000382:microbiology*; D003546:Cysteine Endopeptidases / Q000235:genetics*; D003773:Dental Plaque / Q000382:microbiology*; D005260:Female; D036781:Fimbriae Proteins / Q000235:genetics*; D005838:Genotype; D000080867:Gingipain Cysteine Endopeptidases; D006801:Humans; D008297:Male; D010518:Periodontitis / Q000382:microbiology*; D016966:Porphyromonas gingivalis / Q000145:classification / Q000201:enzymology / Q000235:genetics* / Q000302:isolation & purification*; D055815:Young Adult
chemical_list: D018829:Adhesins, Bacterial; D001426:Bacterial Proteins; D000080867:Gingipain Cysteine Endopeptidases; D036781:Fimbriae Proteins; D003546:Cysteine Endopeptidases
keywords: Porphyromonas gingivalis; chronic periodontitis; fimA; kgp