title: Intravenous AAV9 efficiently transduces myenteric neurons in neonate and juvenile mice.
abstract: Gene therapies for neurological diseases with autonomic or gastrointestinal involvement may require global gene expression. Gastrointestinal complications are often associated with Parkinson's disease and autism. Lewy bodies, a pathological hallmark of Parkinson's brains, are routinely identified in the neurons of the enteric nervous system (ENS) following colon biopsies from patients. The ENS is the intrinsic nervous system of the gut, and is responsible for coordinating the secretory and motor functions of the gastrointestinal tract. ENS dysfunction can cause severe patient discomfort, malnourishment, or even death as in intestinal pseudo-obstruction (Ogilvie syndrome). Importantly, ENS transduction following systemic vector administration has not been thoroughly evaluated. Here we show that systemic injection of AAV9 into neonate or juvenile mice results in transduction of 25-57% of ENS myenteric neurons. Transgene expression was prominent in choline acetyltransferase positive cells, but not within vasoactive intestinal peptide or neuronal nitric oxide synthase cells, suggesting a bias for cells involved in excitatory signaling. AAV9 transduction in enteric glia is very low compared to CNS astrocytes. Enteric glial transduction was enhanced by using a glial specific promoter. Furthermore, we show that AAV8 results in comparable transduction in neonatal mice to AAV9 though AAV1, 5, and 6 are less efficient. These data demonstrate that systemic AAV9 has high affinity for peripheral neural tissue and is useful for future therapeutic development and basic studies of the ENS.
authors.affiliation: Department of Neuroscience, Ohio State University Columbus, OH, USA.
authors.forename: Sara E
authors.initials: SE
authors.lastname: Gombash
authors.affiliation: Department of Neuroscience, Ohio State University Columbus, OH, USA.
authors.forename: Christopher J
authors.initials: CJ
authors.lastname: Cowley
authors.affiliation: Department of Neuroscience, Ohio State University Columbus, OH, USA.
authors.forename: Julie A
authors.initials: JA
authors.lastname: Fitzgerald
authors.affiliation: Department of Neuroscience, Center for Brain and Spinal Cord Repair, Ohio State University Columbus, OH, USA.
authors.forename: Jodie C E
authors.initials: JC
authors.lastname: Hall
authors.affiliation: Department of Pediatrics, Gene Therapy Center, University of Massachusetts Medical School Worcester, MA, USA.
authors.forename: Christian
authors.initials: C
authors.lastname: Mueller
authors.affiliation: Department of Anesthesiology, Ohio State University Columbus, OH, USA.
authors.forename: Fedias L
authors.initials: FL
authors.lastname: Christofi
authors.affiliation: Department of Neuroscience, Ohio State University Columbus, OH, USA.
authors.forename: Kevin D
authors.initials: KD
authors.lastname: Foust
keywords: AAV9; adeno-associated virus; enteric glia; enteric nervous system; enteric neuropathy; functional gastrointestinal motility disorders; gene therapy; myenteric plexus