title: Recombinant expression and purification of "virus-like" bacterial encapsulin protein cages.
abstract: Ultracentrifugation, particularly the use of sucrose or cesium chloride density gradients, is a highly reliable and efficient technique for the purification of virus-like particles and protein cages. Since virus-like particles and protein cages have a unique size compared to cellular macromolecules and organelles, the rate of migration can be used as a tool for purification. Here we describe a detailed protocol for the purification of recently discovered virus-like assemblies called bacterial encapsulins from Thermotoga maritima and Brevibacterium linens.
authors.affiliation: Laboratory for Biomolecular Nanotechnology, MESA + Institute for Nanotechnology, University of Twente, 217, 7500 AE, Enschede, The Netherlands.
authors.forename: W Frederik
authors.initials: WF
authors.lastname: Rurup
authors.forename: Jeroen J L M
authors.initials: JJ
authors.lastname: Cornelissen
authors.forename: Melissa S T
authors.initials: MS
authors.lastname: Koay
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry* / Q000302:isolation & purification*; D001951:Brevibacterium / Q000737:chemistry; D036103:Nanotechnology; D011994:Recombinant Proteins; D020124:Thermotoga maritima / Q000737:chemistry
chemical_list: D001426:Bacterial Proteins; D011994:Recombinant Proteins