title: Universal probe amplification: multiplex screening technologies for genetic variations.
abstract: In order to achieve multiplex screening of genetic variations, multiplex amplification of target genomic DNA is necessary. Universal amplification technology meets this requirement by simultaneously amplifying a number of different regions within the target genomic DNA using a single pair of universal primers and thus eliminating the limitations associated with the use of multiple pairs of primers. We comprehensively review universal probe amplification and its use with multiplex technologies for the identification of the most representative genetic variation, i. e. single nucleotide polymorphisms. The progress and key issues relating to universal probe amplification are discussed and the representative technologies are summarized with an emphasis on their application for the identification of susceptibility to human diseases.
authors.affiliation: Department of Chemical and Biomolecular Engineering, KAIST, Yuseong-gu, Daejeon, Republic of Korea.
authors.forename: Jung Hun
authors.initials: JH
authors.lastname: Park
authors.forename: Ki Soo
authors.initials: KS
authors.lastname: Park
authors.forename: Kyungmee
authors.initials: K
authors.lastname: Lee
authors.forename: Hyowon
authors.initials: H
authors.lastname: Jang
authors.forename: Hyun Gyu
authors.initials: HG
authors.lastname: Park
mesh_terms: D001709:Biotechnology; D019075:Electrophoresis, Capillary; D014644:Genetic Variation; D015336:Molecular Probe Techniques; D015335:Molecular Probes; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D020411:Oligonucleotide Array Sequence Analysis; D012107:Research Design
chemical_list: D015335:Molecular Probes
keywords: Analytical biotechnology; Genetic variation; Microarrays; Multiplex screening; Universal amplification