title: Discrimination of p53 immunohistochemistry-positive tumors by its staining pattern in gastric cancer.
abstract: Immunohistochemistry staining of p53 is a cheap and simple method to detect aberrant function of p53. However, there are some discrepancies between the result of immunohistochemistry staining and mutation analysis. This study attempted to find a new definition of p53 staining by its staining pattern. Immunohistochemistry staining of p53 and TP53 gene mutation analysis were performed in 148 gastric cancer patients. Also SNP-CGH array analysis was conducted to four cases. Positive staining of p53 was observed in 88 (59.5%) tumors. Tumors with positive p53 staining showed malignant features compared to negative tumors. Mutation of TP53 gene was observed in 29 (19.6%) tumors with higher age and differentiated type. In positive p53 tumors, two types could be distinguished; aberrant type and scattered type. With comparison to TP53 gene mutation analysis, all the scattered type had wild-type TP53 gene (P = 0.0003). SNP-CGH array showed that scattered-type tumors had no change in the structure of chromosome 17. P53-scattered-type staining tumors may reflect a functionally active nonmutated TP53 gene. In interpretation of p53 immunohistochemistry staining, distinguishing p53-positive tumors by their staining pattern may be important in gastric cancer.
authors.affiliation: Department of Surgery and Science, Graduate School of Medical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka City, Fukuoka, 812-8582, Japan.
authors.forename: Koji
authors.initials: K
authors.lastname: Ando
authors.forename: Eiji
authors.initials: E
authors.lastname: Oki
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Saeki
authors.forename: Zhao
authors.initials: Z
authors.lastname: Yan
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Tsuda
authors.forename: Gen
authors.initials: G
authors.lastname: Hidaka
authors.forename: Yuta
authors.initials: Y
authors.lastname: Kasagi
authors.forename: Hajime
authors.initials: H
authors.lastname: Otsu
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Kawano
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Kitao
authors.forename: Masaru
authors.initials: M
authors.lastname: Morita
authors.forename: Yoshihiko
authors.initials: Y
authors.lastname: Maehara
mesh_terms: D000368:Aged; D002886:Chromosomes, Human, Pair 17; D055028:Comparative Genomic Hybridization; D005260:Female; D015870:Gene Expression; D006801:Humans; D007150:Immunohistochemistry; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D009154:Mutation; D009367:Neoplasm Staging; D009389:Neovascularization, Pathologic; D020641:Polymorphism, Single Nucleotide; D013274:Stomach Neoplasms / Q000175:diagnosis / Q000235:genetics / Q000378:metabolism*; D016159:Tumor Suppressor Protein p53 / Q000235:genetics / Q000378:metabolism*
chemical_list: D016159:Tumor Suppressor Protein p53
keywords: Gastric cancer; immunohistochemistry; mutation analysis; p53; staining pattern