title: On-chip controlled surfactant-DNA coil-globule transition by rapid solvent exchange using hydrodynamic flow focusing.
abstract: This paper presents a microfluidic method for precise control of the size and polydispersity of surfactant-DNA nanoparticles. A mixture of surfactant and DNA dispersed in 35% ethanol is focused between two streams of pure water in a microfluidic channel. As a result, a rapid change of solvent quality takes place in the central stream, and the surfactant-bound DNA molecules undergo a fast coil-globule transition. By adjusting the concentrations of DNA and surfactant, fine-tuning of the nanoparticle size, down to a hydrodynamic diameter of 70 nm with a polydispersity index below 0.2, can be achieved with a good reproducibility.
authors.affiliation: Institute of Bioengineering and Nanotechnology , 31 Biopolis Way, The Nanos #04-01, Singapore 138669, Singapore.
authors.forename: Ciprian
authors.initials: C
authors.lastname: Iliescu
authors.forename: Cătălin
authors.initials: C
authors.lastname: Mărculescu
authors.forename: Shrinivas
authors.initials: S
authors.lastname: Venkataraman
authors.forename: Baptiste
authors.initials: B
authors.lastname: Languille
authors.forename: Hanry
authors.initials: H
authors.lastname: Yu
authors.forename: Guillaume
authors.initials: G
authors.lastname: Tresset
mesh_terms: D000818:Animals; D010582:Bacteriophage lambda / Q000737:chemistry; D002417:Cattle; D004247:DNA / Q000737:chemistry*; D057446:Hydrodynamics; D046210:Microfluidic Analytical Techniques; D053758:Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D044367:Phase Transition; D013499:Surface Properties; D013501:Surface-Active Agents / Q000737:chemistry*
chemical_list: D013501:Surface-Active Agents; D004247:DNA