title: Induction of the Escherichia coli yijE gene expression by cystine.
abstract: Cystine is formed from two molecules of the cysteine under oxidized conditions, but is reversibly converted to cysteine by reduction. Growth of Escherichia coli is retarded in the presence of excess cystine. Transcriptome analysis showed 11 up-regulated and 26 down-regulated genes upon exposure to excess cystine. The reporter assay confirmed regulation by cystine of the expression of one up-regulated membrane gene, yijE, and two down-regulated membrane genes, yhdT and yihN. In order to identify the as yet unidentified gene encoding cystine efflux transporter, the putative cystine efflux candidate, yijE gene, was over-expressed. Expression of the yijE gene suppressed the slow growth of E. coli in the presence of high concentration of extracellular cystine. In good agreement, the knock-out of yijE gene increased the sensibility to cystine. These observations altogether imply that the yijE gene is involved in response to cystine in E. coli.
authors.affiliation: a Department of Frontier Bioscience and Research Center for Micro-Nano Technology , Hosei University , Koganei , Japan.
authors.forename: Kaneyoshi
authors.initials: K
authors.lastname: Yamamoto
authors.forename: Gen
authors.initials: G
authors.lastname: Nonaka
authors.forename: Takahiro
authors.initials: T
authors.lastname: Ozawa
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Takumi
authors.forename: Akira
authors.initials: A
authors.lastname: Ishihama
mesh_terms: D003553:Cystine / Q000494:pharmacology*; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics*; D029968:Escherichia coli Proteins / Q000235:genetics*; D020869:Gene Expression Profiling; D055786:Gene Knockout Techniques; D017930:Genes, Reporter / Q000235:genetics; D008565:Membrane Proteins / Q000235:genetics*; D015533:Transcriptional Activation / Q000187:drug effects*
chemical_list: D029968:Escherichia coli Proteins; D008565:Membrane Proteins; C000603332:YijE protein, E coli; D003553:Cystine
keywords: Escherichia coli; cystine; cystine resistance; genome expression; yijE