title: Mapping biological composition through quantitative phase and absorption X-ray ptychography.
abstract: Isolating compositional information in biological X-ray imaging can be problematic as such information is conflated with thickness and density variations when viewing in projection through a sample. We demonstrate an effective method for identifying variations in material composition by simultaneously using the quantitative phase and magnitude images provided through soft X-ray ptychography. Using this approach we show significantly increased contrast and improved reliability of the identification of intracellular features from uncharacterised samples. While demonstrated for X-ray ptychography, this method is immediately applicable to electron and optical microscopy methods where the complex transmission function of the sample is recovered.
authors.affiliation: ARC Centre of Excellence for Coherent X-Ray Science, Department of Physics, La Trobe University, Bundoora 3086, Australia.
authors.forename: Michael W M
authors.initials: MW
authors.lastname: Jones
authors.affiliation: ARC Centre of Excellence for Coherent X-Ray Science, Department of Biochemistry, La Trobe University, Bundoora 3086, Australia.
authors.forename: Kirstin
authors.initials: K
authors.lastname: Elgass
authors.affiliation: ARC Centre of Excellence for Coherent X-Ray Science, Department of Physics, La Trobe University, Bundoora 3086, Australia.
authors.forename: Mark D
authors.initials: MD
authors.lastname: Junker
authors.affiliation: ARC Centre of Excellence for Coherent X-Ray Science, Department of Physics, La Trobe University, Bundoora 3086, Australia.
authors.forename: Mac B
authors.initials: MB
authors.lastname: Luu
authors.affiliation: ARC Centre of Excellence for Coherent X-Ray Science, Department of Biochemistry, La Trobe University, Bundoora 3086, Australia.
authors.forename: Michael T
authors.initials: MT
authors.lastname: Ryan
authors.affiliation: 1] ARC Centre of Excellence for Coherent X-Ray Science, Department of Physics, La Trobe University, Bundoora 3086, Australia [2] Australian Synchrotron, 800 Blackburn Rd, Clayton 3168, Australia [3] ARC Centre of Excellence for Advanced Molecular Imaging, Australian Synchrotron, 800 Blackburn Rd, Clayton 3168, Australia.
authors.forename: Andrew G
authors.initials: AG
authors.lastname: Peele
authors.affiliation: ARC Centre of Excellence for Coherent X-Ray Science, Department of Physics, La Trobe University, Bundoora 3086, Australia.
authors.forename: Grant A
authors.initials: GA
authors.lastname: van Riessen
mesh_terms: D000818:Animals; D058948:Cell Tracking / Q000379:methods*; D003287:Contrast Media / Q000737:chemistry; D004583:Electrons; D005347:Fibroblasts / Q000648:ultrastructure; D051379:Mice; D008853:Microscopy / Q000379:methods*; D057054:Molecular Imaging; D014965:X-Rays
chemical_list: D003287:Contrast Media