title: Coadministration of Pinellia ternata Can Significantly Reduce Aconitum carmichaelii to Inhibit CYP3A Activity in Rats.
abstract: Chuanwu (CW), the mother root of Aconitum carmichaelii Debx., is a traditional Chinese medicine (TCM) for treating traumatic injuries, rheumatoid arthritis, and tumors. CW coadministered with banxia (BX), the root of Pinellia ternata, is also widely prescribed in clinical practice. However, the mechanism of this combination is yet deciphered. Current study aimed to investigate the effects of CW, including raw chuanwu (RCW) and processed chuanwu (PCW) alone, as well as CW coadministered with BX on CYP3A activity. Buspirone (BP) and testosterone (Tes) were used as specific probe substrates in vivo and ex vivo, respectively. CYP3A activity was determined by the metabolites formation ratios from the substrates. Compared with those in the control group, the metabolites formation ratios significantly decreased in the RCW and PCW alone groups, accompanied by a marked decrease in CYP3A protein and mRNA levels. However, there was a significant increase in those ratios in the RCW-BX and PCW-BX groups compared to the RCW and PCW alone groups. The results indicated that both RCW and PCW can inhibit CYP3A activity in rats because of downregulation of CYP3A protein and mRNA levels. Decreases in CYP3A activity can be reversed by coadministration with BX.
authors.affiliation: Institute of Chinese Meteria Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China ; International Institute for Translational Chinese Medicine, Guangzhou University of Chinese Medicine, Guangzhou 510006, China.
authors.forename: Jinjun
authors.initials: J
authors.lastname: Wu
authors.affiliation: Institute of Chinese Meteria Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China ; College of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou 350108, China.
authors.forename: Zaixing
authors.initials: Z
authors.lastname: Cheng
authors.affiliation: International Institute for Translational Chinese Medicine, Guangzhou University of Chinese Medicine, Guangzhou 510006, China.
authors.forename: Lijun
authors.initials: L
authors.lastname: Zhu
authors.affiliation: International Institute for Translational Chinese Medicine, Guangzhou University of Chinese Medicine, Guangzhou 510006, China.
authors.forename: Linlin
authors.initials: L
authors.lastname: Lu
authors.affiliation: International Institute for Translational Chinese Medicine, Guangzhou University of Chinese Medicine, Guangzhou 510006, China.
authors.forename: Guiyu
authors.initials: G
authors.lastname: Zhang
authors.affiliation: International Institute for Translational Chinese Medicine, Guangzhou University of Chinese Medicine, Guangzhou 510006, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Institute of Chinese Meteria Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Institute of Chinese Meteria Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China ; International Institute for Translational Chinese Medicine, Guangzhou University of Chinese Medicine, Guangzhou 510006, China.
authors.forename: Na
authors.initials: N
authors.lastname: Lin
authors.affiliation: International Institute for Translational Chinese Medicine, Guangzhou University of Chinese Medicine, Guangzhou 510006, China.
authors.forename: Zhongqiu
authors.initials: Z
authors.lastname: Liu