title: Analysis of HSV Viral Reactivation in Explants of Sensory Neurons.
abstract: As with all Herpesviruses, Herpes simplex virus (HSV) has both a lytic replication phase and a latency-reactivation cycle. During lytic replication, there is an ordered cascade of viral gene expression that leads to the synthesis of infectious viral progeny. In contrast, latency is characterized by the lack of significant lytic gene expression and the absence of infectious virus. Reactivation from latency is characterized by the re-entry of the virus into the lytic replication cycle and the production of recurrent disease. This unit describes the establishment of the mouse sensory neuron model of HSV-1 latency-reactivation as a useful in vivo system for the analysis of mechanisms involved in latency and reactivation. Assays including the determination of viral yields, immunohistochemical/immunofluorescent detection of viral antigens, and mRNA quantitation are used in experiments designed to investigate the network of cellular and viral proteins regulating HSV-1 lytic infection, latency, and reactivation.
authors.affiliation: Laboratory of Viral Diseases, National Institutes of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland.
authors.forename: Jesse H
authors.initials: JH
authors.lastname: Arbuckle
authors.forename: Anne-Marie W
authors.initials: AM
authors.lastname: Turner
authors.forename: Thomas M
authors.initials: TM
authors.lastname: Kristie
mesh_terms: D000818:Animals; D002522:Chlorocebus aethiops; D003315:Cornea / Q000821:virology; D003316:Corneal Diseases / Q000821:virology; D006561:Herpes Simplex / Q000821:virology; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D011984:Sensory Receptor Cells / Q000502:physiology / Q000821:virology*; D018139:Simplexvirus / Q000502:physiology*; D046509:Tissue Culture Techniques; D014709:Vero Cells; D014776:Virus Cultivation; D017735:Virus Latency / Q000502:physiology*
keywords: Herpes simplex virus; corneal infection; immunohistochemistry; latency model; small-molecule inhibitors; trigeminal ganglion; viral titer