title: Calmodulin-like protein from M. tuberculosis H37Rv is required during infection.
abstract: M. tuberculosis constitutes very sophisticated signaling systems that convert the environment signals into appropriate cellular response and helps the bacilli to overcome the onslaught of host defence mechanisms. Although mycobacterial two-component systems and STPKs have gained lot of attention as virulence factors, mycobacterial calcium signaling has not been very well studied. Calcium signaling has been the primary mechanism in eukaryotes for regulation of kinases, however in prokaryotes auto-phosphorylation of number of kinases has been reported. We have previously reported a small calmodulin-like-protein (CAMLP) from M. tuberculosis regulating enzymes of heterogeneous origin. To understand its role in both viability and virulence, we have assessed the effect of reduced expression of CAMLP coding gene Rv1211 on M. tb growth in vitro and ex vivo. Further, we have also studied the expression profile of Rv1211 in various conditions simulating host microenvironments. Our results highlight the possible role of CAMLP in growth and survival of M. tb during infection.
authors.affiliation: Department of Biochemistry, Sri Venkateswara College, University of Delhi South Campus, New Delhi - 110 021, INDIA.
authors.forename: Meeta J
authors.initials: MJ
authors.lastname: Advani
authors.affiliation: Biomedical Research, The University of Texas Health Center at Tyler, Tyler, Texas- 75708-3154, United States.
authors.forename: Malini
authors.initials: M
authors.lastname: Rajagopalan
authors.affiliation: Department of Biochemistry, Sri Venkateswara College, University of Delhi South Campus, New Delhi - 110 021, INDIA.
authors.forename: P Hemalatha
authors.initials: PH
authors.lastname: Reddy
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D002147:Calmodulin / Q000235:genetics / Q000378:metabolism*; D015870:Gene Expression; D015964:Gene Expression Regulation, Bacterial; D008264:Macrophages / Q000276:immunology / Q000382:microbiology; D009169:Mycobacterium tuberculosis / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D010957:Plasmids / Q000235:genetics; D014376:Tuberculosis / Q000382:microbiology
chemical_list: D001426:Bacterial Proteins; D002147:Calmodulin