title: Bioactive composition, antimicrobial activities and the influence of Agrocybe aegerita (Brig.) Sing on certain quorum-sensing-regulated functions and biofilm formation by Pseudomonas aeruginosa.
abstract: Agrocybe aegerita (Brig.) Sing is a basidiomycete, white rot fungus. Antimicrobial activities and the antiqourum effect on Pseudomonas aeruginosa of an A. aegerita methanolic extract were investigated. The extract showed very good antimicrobial activity against all the tested microorganisms in a dose dependent manner. Effects of the Sub-MIC, MIC and 2MIC of the A. aegerita methanolic extract regulated the virulence factors in the quorum sensing (QS) test, as well as biofilm formation on P. aeruginosa. Sub-inhibitory and inhibitory concentrations of the extract demonstrated the reduction of virulence factors such as pyocyanin production, twitching and swimming motility. The biofilm forming capability of P. aeruginosa PAO1 was also reduced in a concentration-dependent manner. Furthermore, the chemical composition of the methanolic extract was determined considering its phenolic composition. The methanolic extract of A. aegerita can be a very good source of bioactive substances. This research is of great importance due to the prevalence of drug-resistant microorganisms.
authors.affiliation: Institute for Biological Research "Siniša Stanković", University of Belgrade, Bulevar Despota Stefana 142, 11060, Belgrade, Serbia. mris@ibiss.bg.ac.rs.
authors.forename: Jovana
authors.initials: J
authors.lastname: Petrović
authors.forename: Jasmina
authors.initials: J
authors.lastname: Glamočlija
authors.forename: Dejan
authors.initials: D
authors.lastname: Stojković
authors.forename: Miloš
authors.initials: M
authors.lastname: Nikolić
authors.forename: Ana
authors.initials: A
authors.lastname: Ćirić
authors.forename: Angela
authors.initials: A
authors.lastname: Fernandes
authors.forename: Isabel C F R
authors.initials: IC
authors.lastname: Ferreira
authors.forename: Marina
authors.initials: M
authors.lastname: Soković
mesh_terms: D055362:Agrocybe / Q000737:chemistry*; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D018441:Biofilms / Q000187:drug effects*; D010936:Plant Extracts / Q000494:pharmacology*; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000235:genetics / Q000502:physiology; D053038:Quorum Sensing / Q000187:drug effects*; D014675:Vegetables / Q000737:chemistry*; D037521:Virulence Factors / Q000235:genetics / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D010936:Plant Extracts; D037521:Virulence Factors