title: Characterization of mixtures of compounds produced in chlorpromazine aqueous solutions by ultraviolet laser irradiation: their applications in antimicrobial assays.
abstract: The study reports an investigation of the photoproducts obtained by exposure of chlorpromazine hydrochloride in ultrapure water (concentration 2 mg/mL) to a 266-nm laser beam obtained by fourth harmonic generation from a Nd:YAG laser (6-ns full time width at half maximum, 10-Hz pulse repetition rate). The photoproducts were analyzed by steady-state UV-Vis absorption, laser-induced fluorescence, Fourier transform infrared spectroscopy, and liquid chromatography-tandem time-of-flight mass spectroscopy. Two figures showing pathways that take place during irradiation for obtaining the final products are shown. The quantum yield of singlet oxygen generation by chlorpromazine (CPZ) was determined relative to standard Zn-phthalocyanine in dimethyl sulfoxide. To outline the role of fluorescence in photoproducts formation rates, fluorescence quantum yield of CPZ during exposure to 355-nm radiation (third harmonic of the fundamental beam of Nd:YAG laser) was investigated relative to standard Coumarin 1 in ethanol. The CPZ solutions exposed 60 and 240 min to 266-nm laser beam, respectively, were tested against Staphylococcus aureus ATCC 25923 strain. For 25 μL of CPZ samples irradiated 240 min, a higher diameter of inhibition has obtained against the tested strain than for the 60-min exposed ones.
authors.affiliation: National Institute for Laser, Plasma and Radiation Physics, Laser Department, Magurele, Ilfov, 077125, RomaniabUniversity of Bucharest, Faculty of Physics, Magurele, Ilfov, 077125, Romania.
authors.forename: Tatiana
authors.initials: T
authors.lastname: Alexandru
authors.affiliation: National Institute for Laser, Plasma and Radiation Physics, Laser Department, Magurele, Ilfov, 077125, Romania.
authors.forename: Angela
authors.initials: A
authors.lastname: Staicu
authors.affiliation: National Institute for Laser, Plasma and Radiation Physics, Laser Department, Magurele, Ilfov, 077125, Romania.
authors.forename: Alexandru
authors.initials: A
authors.lastname: Pascu
authors.affiliation: National Institute for Research and Development in Chemistry and Petrochemistry, Bucharest, 060021, Romania.
authors.forename: Elena
authors.initials: E
authors.lastname: Radu
authors.affiliation: National Institute for Laser, Plasma and Radiation Physics, Laser Department, Magurele, Ilfov, 077125, RomaniadUniversity of Bucharest, Faculty of Chemistry, Bucharest, 030018, Romania.
authors.forename: Alexandru
authors.initials: A
authors.lastname: Stoicu
authors.affiliation: National Institute for Laser, Plasma and Radiation Physics, Laser Department, Magurele, Ilfov, 077125, Romania.
authors.forename: Viorel
authors.initials: V
authors.lastname: Nastasa
authors.affiliation: National Institute for Laser, Plasma and Radiation Physics, Laser Department, Magurele, Ilfov, 077125, Romania.
authors.forename: Andra
authors.initials: A
authors.lastname: Dinache
authors.affiliation: National Institute for Laser, Plasma and Radiation Physics, Laser Department, Magurele, Ilfov, 077125, RomaniabUniversity of Bucharest, Faculty of Physics, Magurele, Ilfov, 077125, Romania.
authors.forename: Mihai
authors.initials: M
authors.lastname: Boni
authors.affiliation: New University of Lisbon, Unit of Medical Microbiology, Institute of Hygiene and Tropical Medicine, Group of Mycobacteriology, Lisbon, 1349-008 PortugalfNew University of Lisbon, Center from Malaria and Other Tropical Diseases, Institute of Hygiene and Tr.
authors.forename: Leonard
authors.initials: L
authors.lastname: Amaral
authors.affiliation: National Institute for Laser, Plasma and Radiation Physics, Laser Department, Magurele, Ilfov, 077125, RomaniabUniversity of Bucharest, Faculty of Physics, Magurele, Ilfov, 077125, Romania.
authors.forename: Mihail Lucian
authors.initials: ML
authors.lastname: Pascu
mesh_terms: D000890:Anti-Infective Agents / Q000737:chemistry*; D001681:Biological Assay; D002746:Chlorpromazine / Q000737:chemistry*; D002853:Chromatography, Liquid; D003374:Coumarins / Q000737:chemistry; D004121:Dimethyl Sulfoxide / Q000737:chemistry; D000431:Ethanol / Q000737:chemistry; D007211:Indoles / Q000737:chemistry; D054833:Isoindoles; D007834:Lasers; D013058:Mass Spectrometry; D008826:Microbial Sensitivity Tests; D010100:Oxygen / Q000737:chemistry; D026082:Singlet Oxygen / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000378:metabolism; D014466:Ultraviolet Rays; D015032:Zinc / Q000737:chemistry
chemical_list: D000890:Anti-Infective Agents; D003374:Coumarins; D007211:Indoles; D054833:Isoindoles; D026082:Singlet Oxygen; D000431:Ethanol; C030123:coumarin; D015032:Zinc; D010100:Oxygen; D002746:Chlorpromazine; C013647:phthalocyanine; D004121:Dimethyl Sulfoxide