title: Isolation and characterization of microsatellite loci for Bixa orellana, an important source of natural dyes.
abstract: Annatto (Bixa orellana) is a plant native from the American continental tropical zone. The seeds are used to produce a carotenoid-based yellow to orange food coloring. Microsatellite markers were developed for the Brazilian native species Bixa orellana to describe its genetic diversity and structure as well as to support conservation studies. Twenty-five microsatellite loci were isolated and characterized using an enriched genomic library. Ten loci were polymorphic in the 50 accessions sampled in this study, while 15 were considered monomorphic. The mean number of alleles per locus was 3.8, ranging from 2 to 6 alleles per locus. Mean values for the observed and expected heterozygosities were 0.541 (ranging from 0 to 0.658) and 0.639 (ranging from 0.422 to 0.787), respectively. All markers described in this study will be useful in further studies evaluating the genetic diversity, population dynamics, and conservation genetics of Bixa orellana.
authors.affiliation: Departamento de Genética, Escola Superior de Agricultura "Luiz de Queiroz", Universidade de São Paulo, Piracicaba, SP, Brasil gabriel.d@usp.br.
authors.forename: G
authors.initials: G
authors.lastname: Dequigiovanni
authors.affiliation: Departamento de Genética, Escola Superior de Agricultura "Luiz de Queiroz", Universidade de São Paulo, Piracicaba, SP, Brasil.
authors.forename: S L F
authors.initials: SL
authors.lastname: Ramos
authors.affiliation: Agência Paulista de Tecnologia dos Agronegócios, Pólo Apta Centro Sul, Piracicaba, SP, Brasil.
authors.forename: M I
authors.initials: MI
authors.lastname: Zucchi
authors.affiliation: Departamento de Genética, Escola Superior de Agricultura "Luiz de Queiroz", Universidade de São Paulo, Piracicaba, SP, Brasil.
authors.forename: M M
authors.initials: MM
authors.lastname: Bajay
authors.affiliation: Departamento de Genética, Escola Superior de Agricultura "Luiz de Queiroz", Universidade de São Paulo, Piracicaba, SP, Brasil.
authors.forename: J B
authors.initials: JB
authors.lastname: Pinheiro
authors.affiliation: Centro de Horticultura, Instituto Agronômico, Campinas, SP, Brasil.
authors.forename: E G
authors.initials: EG
authors.lastname: Fabri
authors.affiliation: Departamento de Genética, Escola Superior de Agricultura "Luiz de Queiroz", Universidade de São Paulo, Piracicaba, SP, Brasil.
authors.forename: E A
authors.initials: EA
authors.lastname: Bressan
authors.affiliation: Departamento de Genética, Escola Superior de Agricultura "Luiz de Queiroz", Universidade de São Paulo, Piracicaba, SP, Brasil.
authors.forename: E A
authors.initials: EA
authors.lastname: Veasey
mesh_terms: D000483:Alleles; D031212:Bixaceae / Q000737:chemistry* / Q000145:classification / Q000235:genetics*; D002338:Carotenoids / Q000378:metabolism*; D004396:Coloring Agents / Q000378:metabolism*; D018744:DNA, Plant / Q000737:chemistry / Q000235:genetics; D005787:Gene Frequency; D015698:Genomic Library; D005838:Genotype; D018895:Microsatellite Repeats / Q000235:genetics*; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic; D017422:Sequence Analysis, DNA
chemical_list: D004396:Coloring Agents; D018744:DNA, Plant; D002338:Carotenoids