title: PALMsiever: a tool to turn raw data into results for single-molecule localization microscopy.
abstract: During the past decade, localization microscopy (LM) has transformed into an accessible, commercially available technique for life sciences. However, data processing can be challenging to the non-specialist and care is still needed to produce meaningful results. PALMsiever has been developed to provide a user-friendly means of visualizing, filtering and analyzing LM data. It includes drift correction, clustering, intelligent line profiles, many rendering algorithms and 3D data visualization. It incorporates the main analysis and data processing modalities used by experts in the field, as well as several new features we developed, and makes them broadly accessible. It can easily be extended via plugins and is provided as free of charge open-source software.
authors.affiliation: Design of Biological Systems, Systems Biology, Center for Genomic Regulation, Dr. Aiguader 88, 08003 Barcelona, Spain, Advanced Light Microscopy Unit, Center for Genomic Regulation, Dr. Aiguader 88, 08003 Barcelona, Spain, and Laboratory for Experimental Biophysics, Institute for Physics of Biological Systems, School of Basic Sciences, Swiss Federal School of Technology, 1015 Lausanne, Switzerland Design of Biological Systems, Systems Biology, Center for Genomic Regulation, Dr. Aiguader 88, 08003 Barcelona, Spain, Advanced Light Microscopy Unit, Center for Genomic Regulation, Dr. Aiguader 88, 08003 Barcelona, Spain, and Laboratory for Experimental Biophysics, Institute for Physics of Biological Systems, School of Basic Sciences, Swiss Federal School of Technology, 1015 Lausanne, Switzerland.
authors.forename: Thomas
authors.initials: T
authors.lastname: Pengo
authors.affiliation: Design of Biological Systems, Systems Biology, Center for Genomic Regulation, Dr. Aiguader 88, 08003 Barcelona, Spain, Advanced Light Microscopy Unit, Center for Genomic Regulation, Dr. Aiguader 88, 08003 Barcelona, Spain, and Laboratory for Experimental Biophysics, Institute for Physics of Biological Systems, School of Basic Sciences, Swiss Federal School of Technology, 1015 Lausanne, Switzerland.
authors.forename: Seamus J
authors.initials: SJ
authors.lastname: Holden
authors.affiliation: Design of Biological Systems, Systems Biology, Center for Genomic Regulation, Dr. Aiguader 88, 08003 Barcelona, Spain, Advanced Light Microscopy Unit, Center for Genomic Regulation, Dr. Aiguader 88, 08003 Barcelona, Spain, and Laboratory for Experimental Biophysics, Institute for Physics of Biological Systems, School of Basic Sciences, Swiss Federal School of Technology, 1015 Lausanne, Switzerland.
authors.forename: Suliana
authors.initials: S
authors.lastname: Manley
mesh_terms: D000465:Algorithms; D016000:Cluster Analysis; D003196:Computer Graphics; D006801:Humans; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D008853:Microscopy / Q000379:methods*; D008870:Microtubules / Q000648:ultrastructure*; D012984:Software; D014584:User-Computer Interface