title: Alteration of gene expression profiling including GPR174 and GNG2 is associated with vasovagal syncope.
abstract: Vasovagal syncope (VVS) causes accidental harm for susceptible patients. However, pathophysiology of this disorder remains largely unknown. In an effort to understanding of molecular mechanism for VVS, genome-wide gene expression profiling analyses were performed on VVS patients at syncope state. A total of 66 Type 1 VVS child patients and the same number healthy controls were enrolled in this study. Peripheral blood RNAs were isolated from all subjects, of which 10 RNA samples were randomly selected from each groups for gene expression profile analysis using Gene ST 1.0 arrays (Affymetrix). The results revealed that 103 genes were differently expressed between the patients and controls. Significantly, two G-proteins related genes, GPR174 and GNG2 that have not been related to VVS were among the differently expressed genes. The microarray results were confirmed by qRT-PCR in all the tested individuals. Ingenuity pathway analysis and gene ontology annotation study showed that the differently expressed genes are associated with stress response and apoptosis, suggesting that the alteration of some gene expression including G-proteins related genes is associated with VVS. This study provides new insight into the molecular mechanism of VVS and would be helpful to further identify new molecular biomarkers for the disease.
authors.affiliation: Shanghai Institute of Medical Genetics, Shanghai Children's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China, huangyj@shchildren.com.cn.
authors.forename: Yu-Juan
authors.initials: YJ
authors.lastname: Huang
authors.forename: Zai-wei
authors.initials: ZW
authors.lastname: Zhou
authors.forename: Miao
authors.initials: M
authors.lastname: Xu
authors.forename: Qing-wen
authors.initials: QW
authors.lastname: Ma
authors.forename: Jing-bin
authors.initials: JB
authors.lastname: Yan
authors.forename: Jian-yi
authors.initials: JY
authors.lastname: Wang
authors.forename: Quo-qin
authors.initials: QQ
authors.lastname: Zhang
authors.forename: Min
authors.initials: M
authors.lastname: Huang
authors.forename: Liming
authors.initials: L
authors.lastname: Bao
mesh_terms: D000293:Adolescent; D016022:Case-Control Studies; D002648:Child; D005260:Female; D019204:GTP-Binding Proteins / Q000235:genetics*; D020869:Gene Expression Profiling / Q000379:methods; D020022:Genetic Predisposition to Disease; D006801:Humans; D008297:Male; D046228:Microarray Analysis / Q000379:methods; D060888:Real-Time Polymerase Chain Reaction; D043562:Receptors, G-Protein-Coupled / Q000235:genetics*; D019462:Syncope, Vasovagal / Q000235:genetics* / Q000503:physiopathology
chemical_list: C410342:GNG2 protein, human; C581018:GPR174 protein, human; D043562:Receptors, G-Protein-Coupled; D019204:GTP-Binding Proteins