title: Anti-inflammatory activity of mushroom-derived hispidin through blocking of NF-κB activation.
abstract: BACKGROUND
  Hispidin, a polyphenol compound mainly derived from the valuable medicinal mushroom Phellinus species, has been found to possess distinct biological effects. However, the anti-inflammatory potential of hispidin still remains uncharacterized.
  
  
  RESULTS
  In this study, the effects of hispidin on activation of nuclear factor kappa B (NF-κB) and the subsequent production of inducible nitric oxide synthase (iNOS) were determined in the lipopolysaccharide (LPS)-induced macrophage RAW 264.7 cells. Our data indicated that hispidin inhibits transcriptional activity of NF-κB in a dose-dependent manner. Hispidin also attenuated LPS-induced NF-κB nuclear translocation and associated inhibitor of kappa B (IκB-α) degradation. Furthermore, hispidin deceased iNOS protein expression and the generation of reactive oxygen species (ROS) in the LPS-induced cells, but did not affect phosphorylation of mitogen-activated protein kinases.
  
  
  CONCLUSIONS
  These findings suggest that hispidin exhibits anti-inflammatory activity through suppressing ROS mediated NF-κB pathway in mouse macrophage cells.
authors.affiliation: Department of Nutrition and Food Science, University of Maryland, College Park, MD 20742, USA.|College of Food Engineering and Nutrition Science, Shaanxi Normal University, Xi'an, 741609, P.R. China.
authors.forename: Hong Jun
authors.initials: HJ
authors.lastname: Shao
authors.affiliation: Department of Nutrition and Food Science, University of Maryland, College Park, MD 20742, USA.
authors.forename: Jin Boo
authors.initials: JB
authors.lastname: Jeong
authors.affiliation: Department of Nutrition and Food Science, University of Maryland, College Park, MD 20742, USA.
authors.forename: Kui-Jin
authors.initials: KJ
authors.lastname: Kim
authors.affiliation: Department of Nutrition and Food Science, University of Maryland, College Park, MD 20742, USA.
authors.forename: Seong-Ho
authors.initials: SH
authors.lastname: Lee
mesh_terms: D000363:Agaricales; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology* / Q000627:therapeutic use; D007249:Inflammation / Q000188:drug therapy; D008262:Macrophage Activation / Q000187:drug effects; D008264:Macrophages / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D016328:NF-kappa B / Q000037:antagonists & inhibitors*; D008517:Phytotherapy; D011753:Pyrones / Q000494:pharmacology* / Q000627:therapeutic use; D000067996:RAW 264.7 Cells; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D000893:Anti-Inflammatory Agents; D016328:NF-kappa B; D011753:Pyrones; D017382:Reactive Oxygen Species; C007946:hispidin
keywords: NF-κB; ROS; anti-inflammation; hispidin