title: In situ labelling chemistry of respiratory syncytial viruses by employing the biotinylated host-cell membrane protein for tracking the early stage of virus entry.
abstract: An in situ labelling strategy was proposed to produce quantum dot-labelled respiratory syncytial viruses (RSVs) by incorporating the biotinylated membrane protein of the host cells into mature virions, followed by conjugation with streptavidin modified quantum dots (SA-QDs), which has the advantages such as convenience, efficiency and minor influence on viral infectivity and thus could be successfully applied to track the early stage of virus entry.
authors.affiliation: Key Laboratory of Luminescent and Real-Time Analytical Chemistry, Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China. chengzhi@swu.edu.cn.
authors.forename: Lin Ling
authors.initials: LL
authors.lastname: Zheng
authors.forename: Xiao Xi
authors.initials: XX
authors.lastname: Yang
authors.forename: Yue
authors.initials: Y
authors.lastname: Liu
authors.forename: Xiao Yan
authors.initials: XY
authors.lastname: Wan
authors.forename: Wen Bi
authors.initials: WB
authors.lastname: Wu
authors.forename: Ting Ting
authors.initials: TT
authors.lastname: Wang
authors.forename: Qiang
authors.initials: Q
authors.lastname: Wang
authors.forename: Shu Jun
authors.initials: SJ
authors.lastname: Zhen
authors.forename: Cheng Zhi
authors.initials: CZ
authors.lastname: Huang
mesh_terms: D001710:Biotin / Q000737:chemistry* / Q000378:metabolism; D019921:Biotinylation; D002460:Cell Line; D005456:Fluorescent Dyes / Q000737:chemistry; D006801:Humans; D008565:Membrane Proteins / Q000737:chemistry* / Q000378:metabolism; D018613:Microscopy, Confocal; D045663:Quantum Dots / Q000737:chemistry*; D012367:RNA, Viral / Q000737:chemistry / Q000378:metabolism; D012136:Respiratory Syncytial Viruses / Q000502:physiology*; D019809:Streptavidin / Q000737:chemistry* / Q000378:metabolism; D013696:Temperature; D059008:Time-Lapse Imaging; D053586:Virus Internalization
chemical_list: D005456:Fluorescent Dyes; D008565:Membrane Proteins; D012367:RNA, Viral; D001710:Biotin; D019809:Streptavidin