title: Possible mechanism of antifungal phenazine-1-carboxamide from Pseudomonas sp. against dimorphic fungi Benjaminiella poitrasii and human pathogen Candida albicans.
abstract: OBJECTIVE
  Investigation of antifungal mechanism of phenazine 1-carboxamide (PC) produced by a Pseudomonas strain MCC2142.
  
  
  RESULTS
  An antifungal metabolite produced by a Pseudomonas was purified and identified as PC. Human pathogenic fungi such as Candida albicans, Candida glabrata, Cryptococcus neoformans, Fusarium oxysporum, Aspergillus fumigatus and Aspergillus niger were found to be inhibited by PC (MIC90 32-64 μg ml(-1)). Addition of PC (20 μg ml(-1)) during yeast (Y)-hypha (H) transitions inhibited germ tube formation by >90% and >99% in C. albicans National Collection of Industrial Microorganisms (NCIM) 3471 and nonpathogenic model Benjaminiella poitrasii, respectively. After exposure to PC (20 μg ml(-1)), 75-80% yeast cells of B. poitrasii and C. albicans NCIM 3471 showed rhodamine 123 fluorescence indicating high intracellular reactive oxygen species (ROS) production. ROS further led to hyperpolarization of mitochondrial membrane, subsequently induction of apoptosis as evident by externalization of phosphatidylserine, DNA fragmentation, chromatin condensation and finally death in B. poitrasii. In C. albicans NCIM 3471, PC (20 μg ml(-1)) induced apoptosis.
  
  
  CONCLUSIONS
  The antifungal effect of PC in B. poitrasii and C. albicans may be due to ROS-mediated apoptotic death.
  
  
  CONCLUSIONS
  Inhibition of Y-H transition of B. poitrasii and C. albicans by PC indicates that it may prove useful in the control of dimorphic human pathogens.
authors.affiliation: Biochemical Sciences Division, CSIR-National Chemical Laboratory, Pune, India.
authors.forename: S G
authors.initials: SG
authors.lastname: Tupe
authors.forename: R R
authors.initials: RR
authors.lastname: Kulkarni
authors.forename: F
authors.initials: F
authors.lastname: Shirazi
authors.forename: D G
authors.initials: DG
authors.lastname: Sant
authors.forename: S P
authors.initials: SP
authors.lastname: Joshi
authors.forename: M V
authors.initials: MV
authors.lastname: Deshpande
mesh_terms: D000935:Antifungal Agents / Q000494:pharmacology*; D017209:Apoptosis; D002176:Candida albicans / Q000187:drug effects*; D009090:Mucorales / Q000187:drug effects* / Q000378:metabolism; D010619:Phenazines / Q000302:isolation & purification / Q000494:pharmacology*; D011549:Pseudomonas / Q000737:chemistry; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D000935:Antifungal Agents; D010619:Phenazines; D017382:Reactive Oxygen Species; C420923:phenazine-1-carboxamide
keywords: Candida albicans; Pseudomonas sp.; apoptosis; dimorphism; phenazines; reactive oxygen species