title: Study of polymorphic variants of the serotonin 2A receptor gene (5-HT2A) and its possible effects on smoking habits of a population from northeastern Brazil.
abstract: Previous studies have revealed a genetic component, including genetic polymorphisms in the serotonergic pathway, particularly in the serotonin receptor gene (5-HT2A). The aim of this study was to investigate associations of the T102C (rs6313) and A-1438G (rs6311) polymorphisms with tobacco use in a population from northeastern Brazil. We evaluated these polymorphisms in 135 nonsmokers and 135 smokers using polymerase chain reaction-restricted fragment length polymorphism. The distribution of allele and genotype frequencies and associations of polymorphisms with smoking were assessed with the chi-squared (χ(2)) test, the Fisher exact test, and odds ratio (OR) with a 95% confidence interval (CI). There were no differences in the distribution of genotype and allele frequencies between nonsmokers and smokers for A-1438G (P = 0.80) and T102C (P = 0.35). However, these polymorphisms were significantly associated with habit frequency (A/G: P = 0.02, OR = 6.87, 95%CI = 1.23-38.31, P = 0.04; A/G+G/G: P = 0.04, OR = 3.67, 95%CI = 1.06-12.75, P = 0.07), age of onset (C/C: P = 0.02, OR = 3.26, 95%CI = 1.17-9.07, P = 0.03, and nicotine dependence level (A/G: P = 0.02, OR = 3.28, 95%CI = 1.17-9.18, P = 0.04; A/G+G/G: P = 0.04, OR = 2.81, 95%CI = 1.13-6.99, P = 0.04; T/C: P = 0.03, OR = 3.12, 95%CI = 1.13-8.57, P = 0.04; T/C+C/C: P = 0.02, OR = 3.06, 95%CI = 1.22-7.70, P = 0.02). Therefore, these polymorphisms may not contribute significantly to smoking initiation, they do appear to be associated with habit maintenance.
authors.affiliation: Laboratório de Genética e Biologia Molecular, Universidade Federal do Piauí, Parnaíba, PI, Brasil.
authors.forename: E S
authors.initials: ES
authors.lastname: Ramos Neto
authors.affiliation: Laboratório de Genética e Biologia Molecular, Universidade Federal do Piauí, Parnaíba, PI, Brasil.
authors.forename: J O
authors.initials: JO
authors.lastname: Mágulas
authors.affiliation: Laboratório de Genética e Biologia Molecular, Universidade Federal do Piauí, Parnaíba, PI, Brasil.
authors.forename: J J S
authors.initials: JJ
authors.lastname: Sousa
authors.affiliation: Laboratório de Genética e Biologia Molecular, Universidade Federal do Piauí, Parnaíba, PI, Brasil.
authors.forename: A C M
authors.initials: AC
authors.lastname: Moura
authors.affiliation: Laboratório de Genética e Biologia Molecular, Universidade Federal do Piauí, Parnaíba, PI, Brasil.
authors.forename: G R
authors.initials: GR
authors.lastname: Pinto
authors.affiliation: Laboratório de Genética e Biologia Molecular, Universidade Federal do Piauí, Parnaíba, PI, Brasil.
authors.forename: F K N
authors.initials: FK
authors.lastname: Yoshioka
authors.affiliation: Laboratório de Genética e Biologia Molecular, Universidade Federal do Piauí, Parnaíba, PI, Brasil.
authors.forename: R
authors.initials: R
authors.lastname: Canalle
authors.affiliation: Laboratório de Genética e Biologia Molecular, Universidade Federal do Piauí, Parnaíba, PI, Brasil motta@ufpi.edu.br.
authors.forename: F J N
authors.initials: FJ
authors.lastname: Motta
mesh_terms: D000328:Adult; D000368:Aged; D000483:Alleles; D001938:Brazil; D016022:Case-Control Studies; D005260:Female; D005787:Gene Frequency; D056726:Genetic Association Studies; D005838:Genotype; D006239:Haplotypes; D006801:Humans; D015810:Linkage Disequilibrium; D008297:Male; D008875:Middle Aged; D016017:Odds Ratio; D011110:Polymorphism, Genetic; D020641:Polymorphism, Single Nucleotide; D044402:Receptor, Serotonin, 5-HT2A / Q000235:genetics*; D012907:Smoking / Q000235:genetics*
chemical_list: D044402:Receptor, Serotonin, 5-HT2A