title: Enrichment of maternal diet with conjugated linoleic acids influences desaturases activity and fatty acids profile in livers and hepatic microsomes of the offspring with 7,12-dimethylbenz[a]anthracene-induced mammary tumors.
abstract: The aim of this study was to assess the influence of diet supplementation of pregnant and breast-feeding female Sprague-Dawley rats with conjugated linoleic acids (CLA) on the Δ6- and Δ5-desaturase activity in hepatic microsomes as well as on fatty acids profile and lipids peroxidation in liver and hepatic microsomes of the progeny with chemically induced mammary tumors. Rats were divided into two groups with different diet supplementation (vegetable oil (which did not contain CLA) or CLA). Their female offspring was divided within these groups into two subgroups: (1)--fed the same diet as mothers (K1 - oil, 01 - CLA), and (2)--fed the standard fodder (K2, O2). At 50th day of life, the progeny obtained carcinogenic agent (7,12-dimethylbenz[a]anthracene). Higher supply of CLA in diet of mothers resulted in lower susceptibility to chemically induced mammary tumors in their offspring (p = 0.0322). It also influenced the fatty acids profile in livers and in hepatic microsomes, especially polyunsaturated n3 and n6 fatty acids. CLA inhibited the activity of the desaturases, which confirmed that CLA can reduce the level of arachidonic acid directly, reducing linoleic acid content in membranes, or indirectly, through the regulation of its metabolism. We were unable to confirm or deny the antioxidative properties of CLA. Our results indicate that the higher supply of CLA in mothers' diet during pregnancy and breastfeeding causes their incorporation into tissues of children, changes the efficiency of fatty acids metabolism and exerts health-promoting effect in their adult life reducing the breast cancer risk.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Białek
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Stawarska
authors.forename: Andrzej
authors.initials: A
authors.lastname: Tokarz
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Czuba
authors.forename: Anna
authors.initials: A
authors.lastname: Konarska
authors.forename: Magdalena
authors.initials: M
authors.lastname: Mazurkiewicz
mesh_terms: D015127:9,10-Dimethyl-1,2-benzanthracene; D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D016588:Anticarcinogenic Agents / Q000008:administration & dosage*; D001943:Breast Neoplasms / Q000201:enzymology / Q000517:prevention & control*; D000090943:Delta-5 Fatty Acid Desaturase; D019587:Dietary Supplements; D004195:Disease Models, Animal; D004791:Enzyme Inhibitors / Q000008:administration & dosage*; D044943:Fatty Acid Desaturases / Q000037:antagonists & inhibitors* / Q000378:metabolism; D005260:Female; D007774:Lactation; D044243:Linoleic Acids, Conjugated / Q000008:administration & dosage*; D050602:Linoleoyl-CoA Desaturase / Q000037:antagonists & inhibitors / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000187:drug effects* / Q000201:enzymology; D039382:Maternal Nutritional Physiological Phenomena; D008862:Microsomes, Liver / Q000187:drug effects / Q000201:enzymology; D011247:Pregnancy; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D016588:Anticarcinogenic Agents; D000090943:Delta-5 Fatty Acid Desaturase; D004791:Enzyme Inhibitors; D044243:Linoleic Acids, Conjugated; D015127:9,10-Dimethyl-1,2-benzanthracene; D044943:Fatty Acid Desaturases; D050602:Linoleoyl-CoA Desaturase