title: [Effect of Sox2 on proliferation of cervical squamous cancer cell line SiHa].
abstract: OBJECTIVE
  To investigate the effect and its mechanism of stem cell related transcription factor Sox2 on the proliferation of cervical squamous carcinoma cell line SiHa.
  
  
  METHODS
  Plasmid pIRES-EGFP-Sox2 or empty plasmid (pIRES-EGFP-empty) was stably transfected into SiHa cells. The expression of Sox2 was detected by both RT-PCT and Western blot. The effects of Sox2 on cellular proliferation and cell cycle were studied by MTT assay and flow cytometry (FCM) respectively. The expression of cell cycle related protein CyclinD1 was detected by Western blot.
  
  
  RESULTS
  Compared to SiHa-EGFP cells, the expression of Sox2 was obviously up-regulated in SiHaSox2 cells (P < 0.01). MTT result showed that SiHa-Sox2 cells grew faster than the control cells. The over expression of Sox2 increased the proportion of transfected cells in phase S. The increased expression of CyclinD1 was further detected after the successful expression of Sox2 (P < 0.05).
  
  
  CONCLUSIONS
  Sox2 could enhance the proliferation of cervical squamous cancer cells in the manner of up-regulating CyclinD1 expression.
authors.forename: Jing
authors.initials: J
authors.lastname: Ji
authors.forename: Fen-Ru
authors.initials: FR
authors.lastname: Ning
authors.forename: Hai-Juan
authors.initials: HJ
authors.lastname: Liu
authors.forename: Xing
authors.initials: X
authors.lastname: Wei
authors.forename: Juan
authors.initials: J
authors.lastname: Zhao
authors.forename: Yue-Ling
authors.initials: YL
authors.lastname: Wang
mesh_terms: D002294:Carcinoma, Squamous Cell / Q000378:metabolism* / Q000473:pathology; D002453:Cell Cycle; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D019938:Cyclin D1 / Q000378:metabolism*; D005260:Female; D006801:Humans; D010957:Plasmids; D055748:SOXB1 Transcription Factors / Q000378:metabolism*; D014162:Transfection; D015854:Up-Regulation; D002583:Uterine Cervical Neoplasms / Q000378:metabolism* / Q000473:pathology
chemical_list: C530104:CCND1 protein, human; C527782:SOX2 protein, human; D055748:SOXB1 Transcription Factors; D019938:Cyclin D1