title: [Effect of quercetin on heat shock protein 27 expression in prostate cancer cells].
abstract: OBJECTIVE
  To evaluate the mRNA expression of heat shock protein 27 (HSP27) in different prostate cancer cell lines including RWPE-1, LNCaP, PC-3, and TSU-Pr1 and to further analyze the effect of quercetin on PC-3 cell lines.
  
  
  METHODS
  The in vitro cultured human prostate cancer cell lines RWPE-1, LNCaP, PC-3, and TSU-Pr1 were randomly divided into four groups: control group; negative control group (treated with dimethyl sulfoxide), high-dose group (treated with 150 Μl 0.6 mg/ml quercetin), and low-dose group (treated with 150 Μl 0.3 mg/ml quercetin). The mRNA expression of HSP27 was detected by reverse transcription polymerase chain reaction (RT-PCR) and immunofluorescence staining.
  
  
  RESULTS
  RT-PCR and immunofluorescence staining showed that HSP27 expression were highly dependent on the cell types and increased in an order of RWPE-1, LNCaP, PC-3, and TSU-Pr1 after quercetin treatment. The HSP27 mRNA expression levels were 128%, 110%, 50%, and 60% in control group, negative control group, high-dose group, and low-dose group. Obviously, it was significantly lower in the high-dose group and low-dose group than in the control group (both P<0.05).
  
  
  CONCLUSIONS
  HSP27 expression level is associated with the the degree of prostate cancer. Quercetin may inhibit HSP27 expression in PC-3 cell.
authors.affiliation: Department of Orthopedic Surgery,Hongqi Hospital of Mudanjiang Medical University,Mudanjiang,Heilongjiang 157011,China.
authors.forename: Feng
authors.initials: F
authors.lastname: Yu
authors.affiliation: Department of Orthopedic Surgery,Hongqi Hospital of Mudanjiang Medical University,Mudanjiang,Heilongjiang 157011,China.
authors.forename: Li-li
authors.initials: LL
authors.lastname: Jiang
authors.affiliation: Department of Orthopedic Surgery,Hongqi Hospital of Mudanjiang Medical University,Mudanjiang,Heilongjiang 157011,China.
authors.forename: Yan-cheng
authors.initials: YC
authors.lastname: Di
mesh_terms: D045744:Cell Line, Tumor; D055551:HSP27 Heat-Shock Proteins / Q000378:metabolism*; D006360:Heat-Shock Proteins; D006801:Humans; D008297:Male; D018832:Molecular Chaperones; D011471:Prostatic Neoplasms / Q000378:metabolism / Q000473:pathology*; D011794:Quercetin / Q000494:pharmacology*
chemical_list: D055551:HSP27 Heat-Shock Proteins; C057725:HSPB1 protein, human; D006360:Heat-Shock Proteins; D018832:Molecular Chaperones; D011794:Quercetin