title: The distinct distributions of immunocompetent cells in rat dentin pulp after pulpotomy.
abstract: Pulpotomy involves the removal of the coronal portion of pulp, including the diseased tissue, with the intent of maintaining the vitality of the remaining pulpal tissue via a therapeutic dressing. Once odontoblasts suffer injuries, the differentiation of mesenchymal cells is induced from the precursor cell population in the dental pulp, and these cells are recruited to the injured site to differentiate into odontoblasts. However, the involvement of immunocompetent cells during pulpal regeneration remains unclear. Thus, the purpose of this study was to investigate the properties of macrophages that infiltrated wound healing sites in rats between 1 and 28 days after pulpotomy (dap). During the inflammatory phase, ED1(+) (CD68(+) ) macrophages significantly increased throughout root pulp, especially apical to the demarcation zone, and this population persisted until 3 dap before decreasing gradually until 28 dap. OX6(+) macrophages expressing class II MHC also increased in the apical pulp at 1 dap and declined thereafter. However, OX6(+) cells appeared prior to dentin bridge formation at 3 dap and appeared again apical to the dentin bridge during the healing stage at 14 dap. The shift from ED1(+) cells in the inflammation phase to OX6(+) cells during dentin bridge formation might contribute to wound healing.
authors.affiliation: Division of Oral Pathology, Department of Health Promotion, Kyushu Dental University, 2-6-1 Manazuru, Kokurakita-ku, Kitakyushu, Fukuoka, 803-8580, Japan.
authors.forename: Min
authors.initials: M
authors.lastname: Zhang
authors.forename: Shoichiro
authors.initials: S
authors.lastname: Kokabu
authors.forename: Chihiro
authors.initials: C
authors.lastname: Nakatomi
authors.forename: Goro
authors.initials: G
authors.lastname: Sugiyama
authors.forename: Kou
authors.initials: K
authors.lastname: Matsuo
authors.forename: Eijiro
authors.initials: E
authors.lastname: Jimi
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D003782:Dental Pulp / Q000166:cytology* / Q000378:metabolism; D003804:Dentin / Q000166:cytology* / Q000378:metabolism; D008264:Macrophages / Q000166:cytology* / Q000473:pathology; D008297:Male; D009804:Odontoblasts / Q000166:cytology* / Q000378:metabolism; D011672:Pulpotomy; D051381:Rats; D017208:Rats, Wistar; D014945:Wound Healing
keywords: ED1; OX6; immunocompetent cells; immunohistochemistry; pulpotomy