title: Imaging of lipids and metabolites using nanospray desorption electrospray ionization mass spectrometry.
abstract: Nanospray desorption electrospray ionization (nano-DESI) is an ambient ionization technique that uses localized liquid extraction for mass spectrometry imaging of molecules on surfaces. Nano-DESI enables imaging of ionizable molecules from a sample in its native state without any special sample pretreatment. In this chapter we describe the protocol for nano-DESI imaging of thin tissue sections.
authors.affiliation: Physical Sciences Division, Pacific Northwest National Laboratory, K8-88, 999, Richland, WA, 99352, USA.
authors.forename: Ingela
authors.initials: I
authors.lastname: Lanekoff
authors.forename: Julia
authors.initials: J
authors.lastname: Laskin
mesh_terms: D050356:Lipid Metabolism; D057054:Molecular Imaging / Q000379:methods*; D036103:Nanotechnology / Q000379:methods*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*