title: Evaluation of FTA ® paper for storage of oral meta-genomic DNA.
abstract: OBJECTIVE
  The purpose of the present study was to evaluate the short-term storage of meta-genomic DNA from native oral biofilms on FTA(®) paper.
  
  
  METHODS
  Thirteen volunteers of both sexes received an acrylic splint for intraoral biofilm formation over a period of 48 hours. The biofilms were collected, resuspended in phosphate-buffered saline, and either stored on FTA(®) paper or directly processed by standard laboratory DNA extraction. The nucleic acid extraction efficiencies were evaluated by 16S rDNA targeted SSCP fingerprinting. The acquired banding pattern of FTA-derived meta-genomic DNA was compared to a standard DNA preparation protocol. Sensitivity and positive predictive values were calculated.
  
  
  RESULTS
  The volunteers showed inter-individual differences in their bacterial species composition. A total of 200 bands were found for both methods and 85% of the banding patterns were equal, representing a sensitivity of 0.941 and a false-negative predictive value of 0.059.
  
  
  CONCLUSIONS
  Meta-genomic DNA sampling, extraction, and adhesion using FTA(®) paper is a reliable method for storage of microbial DNA for a short period of time.
authors.affiliation: Department of Prosthetic Denstiry and Biomedical Materials Science, Hannover Medical School , Hannover, Germany .
authors.forename: Magdalena
authors.initials: M
authors.lastname: Foitzik
authors.forename: Sascha N
authors.initials: SN
authors.lastname: Stumpp
authors.forename: Jasmin
authors.initials: J
authors.lastname: Grischke
authors.forename: Jörg
authors.initials: J
authors.lastname: Eberhard
authors.forename: Meike
authors.initials: M
authors.lastname: Stiesch
mesh_terms: D001419:Bacteria / Q000235:genetics* / Q000254:growth & development; D001422:Bacterial Adhesion; D004269:DNA, Bacterial / Q000032:analysis*; D003773:Dental Plaque / Q000382:microbiology; D005260:Female; D006801:Humans; D008297:Male; D054892:Metagenome; D009055:Mouth / Q000382:microbiology*; D011309:Preservation, Biological / Q000379:methods; D012336:RNA, Ribosomal, 16S / Q000032:analysis*; D013048:Specimen Handling / Q000379:methods
chemical_list: D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S