title: The Bioconversion of Red Ginseng Ethanol Extract into Compound K by Saccharomyces cerevisiae HJ-014.
abstract: A β-glucosidase producing yeast strain was isolated from Korean traditional rice wine. Based on the sequence of the YCL008c gene and analysis of the fatty acid composition, the isolate was identified as Saccharomyces cerevisiae strain HJ-014. S. cerevisiae HJ-014 produced ginsenoside Rd, F2, and compound K from the ethanol extract of red ginseng. The production was increased by shaking culture, where the bioconversion efficiency was increased 2-fold compared to standing culture. The production of ginsenoside F2 and compound K was time-dependent and thought to proceed by the transformation pathway of: red ginseng extract→Rd→F2→compound K. The optimum incubation time and concentration of red ginseng extract for the production of compound K was 96 hr and 4.5% (w/v), respectively.
authors.affiliation: Traditional and Biomedical Research Center, Daejeon University, Daejeon 300-716, Korea.
authors.forename: Hak Joo
authors.initials: HJ
authors.lastname: Choi
authors.affiliation: Traditional and Biomedical Research Center, Daejeon University, Daejeon 300-716, Korea.
authors.forename: Eun A
authors.initials: EA
authors.lastname: Kim
authors.affiliation: Traditional and Biomedical Research Center, Daejeon University, Daejeon 300-716, Korea. ; Department of Pathology, College of Korean Medicine, Daejeon University, Daejeon 300-716, Korea.
authors.forename: Dong Hee
authors.initials: DH
authors.lastname: Kim
authors.affiliation: Division of Life Sciences, College of Natural Science, Daejeon University, Daejeon 300-716, Korea.
authors.forename: Kwang-Soo
authors.initials: KS
authors.lastname: Shin
keywords: Bioconversion; Compound K; Red ginseng extract; Saccharomyces cerevisiae HJ-014