title: Utilization of an automated pipetting system in the cell line-based screening of the activity of a DNA-damaging anti-tumour drug.
abstract: The principles of large screening strategies, which are developed by industrial companies, have been recently adopted by researchers in the fields of molecular biology and oncology as invaluable tools for translational medicine. The declining costs of laboratory robotic machines have allowed high-throughput screening to become more available to academic centres with limited resources. Here, we describe how a robotic conventional liquid handling system could be used on a daily basis in laboratories to obtain consistent and reproducible results. Our approach allowed us to quickly screen a panel of more than 20 tumorigenic and non-tumorigenic cell lines for their responses to hydroxyurea, which is a DNA-damaging anticancer therapeutic drug. The format of 384-well microplates was used for manual cell seeding, and the effect of hydroxyurea was screened at multiple concentrations. The fluorescence-based CyQuant assay was employed as the readout method to analyse the cellular DNA content. The effectiveness of our approach was demonstrated in the experimental results.
authors.affiliation: Institute of Biophysics, Academy of Sciences of the Czech Republic, v. v. i., Brno, Czech Republic.
authors.forename: T
authors.initials: T
authors.lastname: Suchánková
authors.affiliation: Institute of Biostatistics and Analyses, Masaryk University, Brno, Czech Republic.
authors.forename: P
authors.initials: P
authors.lastname: Ovesná
authors.affiliation: National Centre for Biomolecular Research, Faculty of Science and Department of Biology, Faculty of Medicine, Masaryk University, Brno, Czech Republic.
authors.forename: P
authors.initials: P
authors.lastname: Samadder
authors.affiliation: Institute of Biophysics, Academy of Sciences of the Czech Republic, v. v. i., Brno, Czech Republic.
authors.forename: K
authors.initials: K
authors.lastname: Souček
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D001331:Automation; D063646:Carcinogenesis / Q000187:drug effects / Q000473:pathology; D018929:Cell Culture Techniques / Q000379:methods*; D045744:Cell Line, Tumor; D004249:DNA Damage; D004354:Drug Screening Assays, Antitumor; D006801:Humans; D006918:Hydroxyurea / Q000494:pharmacology; D012044:Regression Analysis
chemical_list: D000970:Antineoplastic Agents; D006918:Hydroxyurea