title: Evaluation of the expression of P-glycoprotein in propoxur-resistant Caco-2 cells.
abstract: There is a great concern about the effect of propoxur, as one of the more common N-methyl carbamate pesticides, on human health due to its extensive use in agricultural and non-agricultural applications. Caco-2 cells became resistant to propoxur, and the resistance was confirmed through MTT assay. Then the cell membrane integrity and P-glycoprotein expression were measured by LDH assay and western blot analysis, respectively and compared to the parent cells.  Contrary to what was expected, the expression of P-glycoprotein in propoxur resistant cells was lower than parent cells.
  
  
  CONCLUSIONS
  This study indicates that the resistance to propoxur may not be related to P-glycoprotein expression directly, since P-glycoprotein expression has decreased in these cells.
authors.affiliation: Department of Toxicology & Pharmacology, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran. yazdian.shabnam@gmail.com.
authors.forename: Shabnam
authors.initials: S
authors.lastname: Yazdian
authors.affiliation: Department of Pharmacology, Iran Pasteur Institute, Tehran, Iran. fahamn@yahoo.com.
authors.forename: Najmeh
authors.initials: N
authors.lastname: Fahham
authors.affiliation: Department of Toxicology & Pharmacology, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran. mhghahremani@tums.ac.ir.
authors.forename: Mohammad Hossein
authors.initials: MH
authors.lastname: Ghahremani
authors.affiliation: Virtual School, Tehran University of Medical Sciences, Tehran, Iran. monireafzali_t@yahoo.com.
authors.forename: Monireh
authors.initials: M
authors.lastname: Afzali
authors.affiliation: Department of Toxicology & Pharmacology, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran. farsandaj@razi.tums.ac.ir.
authors.forename: Narges
authors.initials: N
authors.lastname: Farsandaj
authors.affiliation: Department of Biology, College of Science, University of Tehran, Tehran, Iran. ostadnas@sina.tums.ac.ir.
authors.forename: Melody
authors.initials: M
authors.lastname: Vatankhah
authors.affiliation: Department of Toxicology and Pharmacology, Toxicology and Poisoning Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran. ostadnas@sina.tums.ac.ir.
authors.forename: Seyed Nasser
authors.initials: SN
authors.lastname: Ostad
mesh_terms: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000235:genetics*; D018938:Caco-2 Cells; D002462:Cell Membrane / Q000378:metabolism*; D004351:Drug Resistance; D006801:Humans; D001074:Propoxur / Q000494:pharmacology*
chemical_list: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D001074:Propoxur