title: MicroRNA-183 inhibits apoptosis and promotes proliferation and invasion of gastric cancer cells by targeting PDCD4.
abstract: MicroRNA plays an important role in multiple processes of cancer development. Aberrant expression of miR-183 has been frequently reported in a variety of cancer types; however, the roles and mechanisms of miR-183 in gastric cancer are largely unknown. Here, we report that miR-183 is significantly up-regulated in human gastric tumor tissues compared to the adjacent normal tissues. Up-regulation of miR-183 is associated with advanced clinical stage, positive lymph node, deep stromal invasion, and distant metastasis in gastric cancer patients. We further demonstrated that miR-183 promotes gastric cancer cell growth in vitro by inhibition of apoptosis. Moreover, overexpression of miR-183 enhances gastric cancer cell migration and invasion. Mechanistically, we demonstrated that overexpression of miR-183 decreased, and inhibition of miR-183 increased the expression of PDCD4, a tumor suppressor, at both mRNA and protein levels. Taken together, our results suggest that miR-183 may modulate progression and metastatic potential of gastric cancer through inhibition of PDCD4 expression. miR-183 could serve as a potential biomarker for gastric cancer progression and a novel therapeutic target for gastric cancer treatment.
authors.affiliation: Department of Gastroenterology, Ruijin Hospital, Luwan Branch, Shanghai Jiaotong University School of Medicine Shanghai, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Gu
authors.affiliation: Department of Geriatrics, Renji Hospital, Shanghai Jiaotong University School of Medicine Shanghai, China.
authors.forename: Tian
authors.initials: T
authors.lastname: Gao
authors.affiliation: Department of Gastroenterology, Shanghai Tenth people's Hospital, Tongji University School of Medicine Shanghai, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Shen
authors.affiliation: Department of Gastroenterology, Ruijin Hospital, Luwan Branch, Shanghai Jiaotong University School of Medicine Shanghai, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Sun
authors.affiliation: Department of Gastroenterology, Ruijin Hospital, Luwan Branch, Shanghai Jiaotong University School of Medicine Shanghai, China.
authors.forename: Xiong
authors.initials: X
authors.lastname: Zheng
authors.affiliation: Department of Gastroenterology, Ruijin Hospital, Luwan Branch, Shanghai Jiaotong University School of Medicine Shanghai, China.
authors.forename: Ji
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Gastroenterology, Ruijin Hospital, Luwan Branch, Shanghai Jiaotong University School of Medicine Shanghai, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Ma
authors.affiliation: Department of Gastroenterology, Ruijin Hospital, Luwan Branch, Shanghai Jiaotong University School of Medicine Shanghai, China.
authors.forename: Xiao-Ying
authors.initials: XY
authors.lastname: Hu
authors.affiliation: Department of Gastroenterology, Ruijin Hospital, Luwan Branch, Shanghai Jiaotong University School of Medicine Shanghai, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Gastroenterology, Ruijin Hospital, Luwan Branch, Shanghai Jiaotong University School of Medicine Shanghai, China.
authors.forename: Mei-Jie
authors.initials: MJ
authors.lastname: Hu
keywords: Gastric cancer; PDCD4; metastasis; miR-183