title: Phaeobacter marinintestinus sp. nov., isolated from the intestine of a sea cucumber (Apostichopus japonicus).
abstract: A Gram-negative, strictly aerobic, non-motile, and rod-shaped bacterial strain designated UB-M7(T) was isolated from the intestine of a sea cucumber (Apostichopus japonicus) collected from Pohang in South Korea. Strain UB-M7(T) displayed optimal growth at 25 °C, pH 7.0-7.5, and with 2.0-3.0 % (w/v) NaCl. Phylogenetic analyses based on the 16S rRNA gene sequences showed that strain UB-M7(T) clustered with Phaeobacter arcticus DSM 23566(T), Phaeobacter inhibens DSM 16374(T), Phaeobacter gallaeciensis BS107(T), and Phaeobacter leonis 306(T), exhibiting 16S rRNA gene sequence similarity values of 96.8, 96.6, 96.4, and 96.2 %, respectively. Strain UB-M7(T) was found to exhibit the highest gyrB sequence similarity value of 80.6 % to the type strain of P. arcticus. The major respiratory quinone of strain UB-M7(T) was found to be ubiquinone 10 (Q-10). The major cellular fatty acids (>5 % of the total fatty acids) are summed features 8 (comprising C18:1 ω7c and/or C18:1 ω6c), 11-methyl C18:1 ω7c, and cyclo C19:0 ω8c. The DNA G+C content was found to be 58.5 mol% and DNA-DNA relatedness value with P. arcticus JCM 14644(T) was 17.2 ± 2.4 %. The major polar lipids of strain UB-M7(T) were identified as phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylcholine; one phospholipid, and three other lipids remain unidentified. Based on its phenotypic, phylogenetic, and chemotaxonomic properties it is concluded that strain UB-M7(T) represents a novel species in the genus Phaeobacter, for which the name Phaeobacter marinintestinus sp. nov. is proposed. The type strain is UB-M7(T) (=KCCM 43045(T) = JCM 19926(T)).
authors.affiliation: Fermentation Research Group, Korea Food Research Institute (KFRI), Sungnam, 463-746, Republic of Korea.
authors.forename: Mi-Hwa
authors.initials: MH
authors.lastname: Lee
authors.forename: Eun-Ji
authors.initials: EJ
authors.lastname: Song
authors.forename: Myung-Ji
authors.initials: MJ
authors.lastname: Seo
authors.forename: Dong-Wook
authors.initials: DW
authors.lastname: Hyun
authors.forename: Jin-Woo
authors.initials: JW
authors.lastname: Bae
authors.forename: So-Young
authors.initials: SY
authors.lastname: Lee
authors.forename: Seong Woon
authors.initials: SW
authors.lastname: Roh
authors.forename: Young-Do
authors.initials: YD
authors.lastname: Nam
mesh_terms: D000332:Aerobiosis; D000818:Animals; D015373:Bacterial Typing Techniques; D001482:Base Composition; D016000:Cluster Analysis; D003600:Cytosol / Q000737:chemistry; D027081:DNA Gyrase / Q000235:genetics; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D007422:Intestines / Q000382:microbiology; D007723:Korea; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D011809:Quinones / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D041902:Rhodobacteraceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride / Q000378:metabolism; D047528:Stichopus / Q000382:microbiology*; D013696:Temperature
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D010743:Phospholipids; D011809:Quinones; D012336:RNA, Ribosomal, 16S; D012965:Sodium Chloride; D027081:DNA Gyrase