title: Recovery of drugs of abuse from Dräger DCD5000 oral fluid collection device in Australia.
abstract: In Australia, it is a requirement of workplace oral fluid (OF) drugs of abuse testing that drug recovery from collection devices be verified by an accredited laboratory. Recovery data are used in conjunction with collection volume imprecision data and uncertainty of measurement to provide an estimation of drug concentration in neat OF. The manufacturer's product information for the DCD5000 collection device indicates that the collection volume of the swab is 380 µL. Recovery data for the swab when used with the isopropanol provided by the manufacturer are not available. A series of experiments using fortified drug-free OF were performed to assess the collection volume imprecision of the Dräger DCD5000 swab and the recovery of drugs from the swab using isopropanol. The fortified OF was collected with the swabs (n = 16), and swabs were discharged into vials of isopropanol as per the manufacturer's instructions. The mean collection volume of the DCD5000 swab was 487 µL with an imprecision of 1.3%. Recovery of drug from the device ranged from 86 to 98% for drugs listed in the Australian OF workplace testing standard. Recovery of methadone, buprenorphine and norbuprenorphine ranged from 93 to 102%. Recovery of 2-ethylidene-1,5-dimethyl-3,3-diphenylpyrrolidene was 45%, suggesting that urine is more suitable sample if methadone therapy is being monitored. Overall, drug recovery from the device using isopropanol was acceptable when the increased collection volume of the swab was taken into account.
authors.affiliation: Drugs of Abuse Laboratory, Clinical Chemistry, Level 6, Mater Adults Hospital, Raymond Terrace, South Brisbane, QLD 4101, Australia ashley.hall@mater.org.au.
authors.forename: Ashley-Jane
authors.initials: AJ
authors.lastname: Hall
authors.affiliation: Drugs of Abuse Laboratory, Clinical Chemistry, Level 6, Mater Adults Hospital, Raymond Terrace, South Brisbane, QLD 4101, Australia.
authors.forename: Janet V
authors.initials: JV
authors.lastname: Warner
authors.affiliation: Drugs of Abuse Laboratory, Clinical Chemistry, Level 6, Mater Adults Hospital, Raymond Terrace, South Brisbane, QLD 4101, Australia.
authors.forename: Michael G
authors.initials: MG
authors.lastname: Henman
authors.affiliation: Drugs of Abuse Laboratory, Clinical Chemistry, Level 6, Mater Adults Hospital, Raymond Terrace, South Brisbane, QLD 4101, Australia.
authors.forename: Wendy E
authors.initials: WE
authors.lastname: Ferguson
mesh_terms: D019840:2-Propanol / Q000032:analysis; D001315:Australia; D006801:Humans; D015813:Substance Abuse Detection / Q000295:instrumentation*
chemical_list: D019840:2-Propanol