title: Identification and molecular characterization of a chitin-binding protein from the beet webworm, Loxostege sticticalis L.
abstract: As the first crucial barrier in the midgut of insects, the peritrophic membrane (PM) plays an important role in preventing external invasion. PM proteins, as the major components of the PM, determine the structure and function of this membrane. A new PM protein, named LstiCBP, from the PM of Loxostege sticticalis larvae was identified using cDNA library screening. The full cDNA of LstiCBP is 2606 bp in length and contains a 2403 bp ORF that encodes an 808-amino acid preprotein with a 15-amino acid as signal peptide. The deduced protein sequence of the cDNA contains 8 cysteine-rich chitin-binding domains (CBDs). Recombinant LstiCBP was successfully expressed in BL21 cells using recombinant plasmid DNA and showed high chitin-binding activity. LstiCBP expression was detected in the midgut at both the transcriptional and translational levels; however, the biochemical and physiological functions of LstiCBP in L. sticticalis require further investigation.
authors.affiliation: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China. jyin@ippcaas.cn.
authors.forename: Jiao
authors.initials: J
authors.lastname: Yin
authors.affiliation: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China. tinyys@126.com.
authors.forename: Shuang
authors.initials: S
authors.lastname: Yang
authors.affiliation: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China. kbli@ippcaas.cn.
authors.forename: Kebin
authors.initials: K
authors.lastname: Li
authors.affiliation: Plant Science and Technology College, Beijing University of Agriculture, Beijing 102206, China. guoweibua@163.com.
authors.forename: Wei
authors.initials: W
authors.lastname: Guo
authors.affiliation: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China. yzcao@ippcaas.cn.
authors.forename: Yazhong
authors.initials: Y
authors.lastname: Cao
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D027461:Beta vulgaris / Q000469:parasitology*; D002352:Carrier Proteins / Q000235:genetics* / Q000378:metabolism*; D002686:Chitin / Q000378:metabolism*; D003001:Cloning, Molecular / Q000379:methods; D018076:DNA, Complementary; D015723:Gene Library; D019476:Insect Proteins / Q000235:genetics* / Q000378:metabolism*; D007814:Larva / Q000378:metabolism; D007915:Lepidoptera / Q000235:genetics*; D008969:Molecular Sequence Data; D016415:Sequence Alignment
chemical_list: D002352:Carrier Proteins; D018076:DNA, Complementary; D019476:Insect Proteins; D002686:Chitin