title: [Regulative effect of bakuchiol on ESF-1 cells anti-aging gene].
abstract: OBJECTIVE
  To explore the mechanism of bakuchiol on anti-aging gene mRNA expression level of human skin fibroblasts (ESF-1).
  
  
  METHODS
  The potential of cell proliferation which was divided into blank group,positive control estradiol group, and bakuchiol high, medium and low dose groups was detected by MTT. The expression levels of Col I, Col III, TIMP-1, TIMP-2 and MMP-1 mRNA were detected with RT-PCR.
  
  
  RESULTS
  ESF-1 vitality and the expression levels of Col I, Col III, TIMP-1 and TIMP-2 mRNA were significantly increased by bakuchiol and E2. However, the expression of MMP-1 mRNA was reduced by bakuchiol and E2.
  
  
  CONCLUSIONS
  The bakuchiol can enhance ESF-1 cell activity, promote collagen and matrix metalloproteinase inhibitors mRNA expression level and inhibit mRNA expression of matrix metalloproteinases in order to postpone skin aging.
authors.forename: Qian
authors.initials: Q
authors.lastname: Yu
authors.forename: Hai-Man
authors.initials: HM
authors.lastname: Zou
authors.forename: Shuai
authors.initials: S
authors.lastname: Wang
authors.forename: Yan-Ming
authors.initials: YM
authors.lastname: Xu
authors.forename: Jian-Min
authors.initials: JM
authors.lastname: Li
authors.forename: Ning
authors.initials: N
authors.lastname: Zhang
mesh_terms: D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D003094:Collagen / Q000235:genetics / Q000378:metabolism*; D024042:Collagen Type I / Q000235:genetics / Q000378:metabolism; D024061:Collagen Type III / Q000235:genetics / Q000378:metabolism; D004958:Estradiol / Q000494:pharmacology; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D020781:Matrix Metalloproteinase 1 / Q000235:genetics / Q000378:metabolism; D010636:Phenols / Q000008:administration & dosage / Q000494:pharmacology*; D029907:Psoralea / Q000737:chemistry; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012867:Skin / Q000166:cytology*; D015595:Skin Aging / Q000187:drug effects; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000235:genetics / Q000378:metabolism; D019716:Tissue Inhibitor of Metalloproteinase-2 / Q000235:genetics / Q000378:metabolism; D019714:Tissue Inhibitor of Metalloproteinases / Q000235:genetics / Q000378:metabolism*
chemical_list: D024042:Collagen Type I; D024061:Collagen Type III; D010636:Phenols; D012333:RNA, Messenger; C560822:TIMP1 protein, human; C560823:TIMP2 protein, human; D019715:Tissue Inhibitor of Metalloproteinase-1; D019714:Tissue Inhibitor of Metalloproteinases; D019716:Tissue Inhibitor of Metalloproteinase-2; D004958:Estradiol; D003094:Collagen; C522360:MMP1 protein, human; D020781:Matrix Metalloproteinase 1; C012765:bakuchiol