title: Zoogloea oleivorans sp. nov., a floc-forming, petroleum hydrocarbon-degrading bacterium isolated from biofilm.
abstract: A floc-forming, Gram-stain-negative, petroleum hydrocarbon-degrading bacterial strain, designated Buc(T), was isolated from a petroleum hydrocarbon-contaminated site in Hungary. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain Buc(T) formed a distinct phyletic lineage within the genus Zoogloea. Its closest relative was found to be Zoogloea caeni EMB43(T) (97.2% 16S rRNA gene sequence similarity) followed by Zoogloea oryzae A-7(T) (95.9%), Zoogloea ramigera ATCC 19544(T) (95.5%) and Zoogloea resiniphila DhA-35(T) (95.4%). The level of DNA-DNA relatedness between strain Buc(T) and Z. caeni EMB43(T) was 31.6%. Cells of strain Buc(T) are facultatively aerobic, rod-shaped, and motile by means of a polar flagellum. The strain grew at temperatures of 5-35 °C (optimum 25-28 °C), and at pH 6.0-9.0 (optimum 6.5-7.5). The predominant fatty acids were C16:0, C10 : 0 3-OH, C12:0 and summed feature 3 (C16 : 1ω7c and/or iso-C15 : 0 2-OH). The major respiratory quinone was ubiquinone-8 (Q-8) and the predominant polar lipid was phosphatidylethanolamine. The genomic DNA G+C content was 63.2 mol%. On the basis of the chemotaxonomic, molecular and phenotypic data, isolate Buc(T) is considered to represent a novel species of the genus Zoogloea, for which the name Zoogloea oleivorans sp. nov. is proposed. The type strain is Buc(T) ( =DSM 28387(T) =NCAIM B 02570(T)).
authors.affiliation: Regional University Center of Excellence in Environmental Industry, Szent István University, Gödöllő, Hungary.|Department of Environmental Protection and Environmental Safety, Szent István University, Gödöllő, Hungary.
authors.forename: Milán
authors.initials: M
authors.lastname: Farkas
authors.affiliation: Regional University Center of Excellence in Environmental Industry, Szent István University, Gödöllő, Hungary.
authors.forename: András
authors.initials: A
authors.lastname: Táncsics
authors.affiliation: Department of Environmental Protection and Environmental Safety, Szent István University, Gödöllő, Hungary.
authors.forename: Balázs
authors.initials: B
authors.lastname: Kriszt
authors.affiliation: Regional University Center of Excellence in Environmental Industry, Szent István University, Gödöllő, Hungary.
authors.forename: Tibor
authors.initials: T
authors.lastname: Benedek
authors.affiliation: Department of Microbiology, Eötvös Loránd University, Budapest, Hungary.
authors.forename: Erika M
authors.initials: EM
authors.lastname: Tóth
authors.affiliation: Department of Microbiology, Eötvös Loránd University, Budapest, Hungary.
authors.forename: Zsuzsa
authors.initials: Z
authors.lastname: Kéki
authors.affiliation: Department of Environmental Protection and Environmental Safety, Szent István University, Gödöllő, Hungary.
authors.forename: Péter G
authors.initials: PG
authors.lastname: Veres
authors.affiliation: Department of Environmental Protection and Environmental Safety, Szent István University, Gödöllő, Hungary.
authors.forename: Sándor
authors.initials: S
authors.lastname: Szoboszlay
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D001673:Biodegradation, Environmental; D018441:Biofilms; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D006814:Hungary; D006838:Hydrocarbons / Q000378:metabolism; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010578:Petroleum / Q000382:microbiology*; D010714:Phosphatidylethanolamines / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D014451:Ubiquinone / Q000737:chemistry; D015045:Zoogloea / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D006838:Hydrocarbons; D010578:Petroleum; D010714:Phosphatidylethanolamines; D012336:RNA, Ribosomal, 16S; D014451:Ubiquinone; C483858:phosphatidylethanolamine; C030778:ubiquinone 8