title: Homogeneous electrochemical assay for protein kinase activity.
abstract: Herein, we report a homogeneous assay for protein kinase activity using an electrochemistry-based probe. The approach involves a peptide substrate conjugated with a redox tag and the phosphate-specific receptor immobilized on an electrode surface. The peptide substrate phosphorylated by a protein kinase binds to the receptor site of the probe, which results in a redox current under voltammetric measurement. Our method was successfully applied even in the presence of citrated human blood and modified to enable a single-use, chip-based electrochemical assay for kinase activity.
authors.affiliation: Department of Chemistry, Seoul National University , Seoul 151-747, Republic of Korea.
authors.forename: Ik-Soo
authors.initials: IS
authors.lastname: Shin
authors.forename: Rohit
authors.initials: R
authors.lastname: Chand
authors.forename: Sang Wook
authors.initials: SW
authors.lastname: Lee
authors.forename: Hyun-Woo
authors.initials: HW
authors.lastname: Rhee
authors.forename: Yong-Sang
authors.initials: YS
authors.lastname: Kim
authors.forename: Jong-In
authors.initials: JI
authors.lastname: Hong
mesh_terms: D055664:Electrochemical Techniques; D006801:Humans; D011494:Protein Kinases / Q000032:analysis* / Q000378:metabolism*
chemical_list: D011494:Protein Kinases