title: Label-free hyperspectral nonlinear optical microscopy of the biofuel micro-algae Haematococcus Pluvialis.
abstract: We consider multi-modal four-wave mixing microscopies to be ideal tools for the in vivo study of carotenoid distributions within the important biofuel microalgae Haematococcus pluvialis. We show that hyperspectral coherent anti-Stokes Raman scattering (CARS) microscopy generates non-invasive, quantitative real-time concentrations maps of intracellular carotenoid distributions in live algae.
authors.affiliation: Department of Physics, University of Ottawa, Ottawa ON, Canada ; Security & Disruptive Technologies, National Research Council, 100 Sussex Dr., Ottawa ON, Canada.
authors.forename: Aaron M
authors.initials: AM
authors.lastname: Barlow
authors.affiliation: Department of Physics & Astronomy, Trent University, Peterborough ON, Canada ; aaronslepkov@trentu.ca.
authors.forename: Aaron D
authors.initials: AD
authors.lastname: Slepkov
authors.affiliation: Security & Disruptive Technologies, National Research Council, 100 Sussex Dr., Ottawa ON, Canada.
authors.forename: Andrew
authors.initials: A
authors.lastname: Ridsdale
authors.affiliation: Algal Carbon Conversion Flagship Program, National Research Council, Halifax, N.S., Canada.
authors.forename: Patrick J
authors.initials: PJ
authors.lastname: McGinn
authors.affiliation: Department of Physics, University of Ottawa, Ottawa ON, Canada ; Security & Disruptive Technologies, National Research Council, 100 Sussex Dr., Ottawa ON, Canada.
authors.forename: Albert
authors.initials: A
authors.lastname: Stolow
keywords: (170.1420) Biology; (170.1530) Cell analysis; (180.4315) Nonlinear microscopy; (300.6230) Spectroscopy, coherent anti-Stokes Raman scattering; (300.6290) Spectroscopy, four-wave mixing