title: BPTF Associated with EMT Indicates Negative Prognosis in Patients with Hepatocellular Carcinoma.
abstract: OBJECTIVE
  Epithelial-mesenchymal transition (EMT) plays an important role in hepatocellular carcinoma (HCC) dissemination. Bromodomain PHD-finger transcription factor (BPTF) could regulate embrogenesis and stem cell differentiation, and it may be involved in tumor progression and EMT. In this study, we aimed to determine BPTF, E-cadherin and vimentin expression in tumor tissues and the clinical significance in relation to HCC.
  
  
  METHODS
  The BPTF, vimentin and E-cadherin expression of 106 HCC tissue samples was examined by immunohistochemical staining.
  
  
  RESULTS
  BPTF and vimentin showed high expression and E-cadherin showed low expression in HCC. BPTF is associated with the tumor number, vascular invasion, Edmondson-Steiner grade, TNM stage and recurrence (P < 0.05). Vimentin is positively correlated with tumor size, tumor number, vascular invasion, Edmondson-Steiner grade, TNM stage and recurrence (P < 0.05). E-cadherin is negatively correlated with tumor number, Edmondson-Steiner grade, TNM stage and recurrence (P < 0.05). Survival analysis has shown that high expression of BPTF and vimentin indicates poorer overall and disease-free survival (P < 0.05). Multivariate analysis shows that BPTF is an independent marker for survival prediction (P = 0.015). Additionally, high BPTF expression is correlated with high vimentin expression and low E-cadherin expression (P < 0.05).
  
  
  CONCLUSIONS
  High BPTF expression may be an independent marker for survival prediction in HCC patients and is probably involved in EMT.
authors.affiliation: Department of General Surgery, Affiliated Nanhua Hospital of University of South China, 336 South Dongfeng Road, Hengyang, 421002, Hunan Province, China.
authors.forename: Shuai
authors.initials: S
authors.lastname: Xiao
authors.forename: Longfei
authors.initials: L
authors.lastname: Liu
authors.forename: Min
authors.initials: M
authors.lastname: Fang
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Zhou
authors.forename: Xiuda
authors.initials: X
authors.lastname: Peng
authors.forename: Jianwu
authors.initials: J
authors.lastname: Long
authors.forename: Xianzhou
authors.initials: X
authors.lastname: Lu
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D034961:Antigens, Nuclear / Q000378:metabolism*; D014408:Biomarkers, Tumor / Q000378:metabolism*; D015820:Cadherins / Q000378:metabolism; D006528:Carcinoma, Hepatocellular / Q000378:metabolism* / Q000401:mortality / Q000473:pathology; D002681:China / Q000453:epidemiology; D058750:Epithelial-Mesenchymal Transition; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D008113:Liver Neoplasms / Q000378:metabolism* / Q000401:mortality / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D011379:Prognosis; D014157:Transcription Factors / Q000378:metabolism*; D014746:Vimentin / Q000378:metabolism; D055815:Young Adult; D000092468:Bromodomain Containing Proteins
chemical_list: D034961:Antigens, Nuclear; D014408:Biomarkers, Tumor; D015820:Cadherins; D009419:Nerve Tissue Proteins; D014157:Transcription Factors; D014746:Vimentin; C406238:fetal Alzheimer antigen; D000092468:Bromodomain Containing Proteins