title: Surfactant titration of nanoparticle-protein corona.
abstract: Nanoparticles (NP), when exposed to biological fluids, are coated by specific proteins that form the so-called protein corona. While some adsorbing proteins exchange with the surroundings on a short time scale, described as a "dynamic" corona, others with higher affinity and long-lived interaction with the NP surface form a "hard" corona (HC), which is believed to mediate NP interaction with cellular machineries. In-depth NP protein corona characterization is therefore a necessary step in understanding the relationship between surface layer structure and biological outcomes. In the present work, we evaluate the protein composition and stability over time and we systematically challenge the formed complexes with surfactants. Each challenge is characterized through different physicochemical measurements (dynamic light scattering, ζ-potential, and differential centrifugal sedimentation) alongside proteomic evaluation in titration type experiments (surfactant titration). 100 nm silicon oxide (Si) and 100 nm carboxylated polystyrene (PS-COOH) NPs cloaked by human plasma HC were titrated with 3-[(3-Cholamidopropyl) dimethylammonio]-1-propanesulfonate (CHAPS, zwitterionic), Triton X-100 (nonionic), sodium dodecyl sulfate (SDS, anionic), and dodecyltrimethylammonium bromide (DTAB, cationic) surfactants. Composition and density of HC together with size and ζ-potential of NP-HC complexes were tracked at each step after surfactant titration. Results on Si NP-HC complexes showed that SDS removes most of the HC, while DTAB induces NP agglomeration. Analogous results were obtained for PS NP-HC complexes. Interestingly, CHAPS and Triton X-100, thanks to similar surface binding preferences, enable selective extraction of apolipoprotein AI (ApoAI) from Si NP hard coronas, leaving unaltered the dispersion physicochemical properties. These findings indicate that surfactant titration can enable the study of NP-HC stability through surfactant variation and also selective separation of certain proteins from the HC. This approach thus has an immediate analytical value as well as potential applications in HC engineering.
authors.affiliation: Centre for BioNano Interactions, School of Chemistry and Chemical Biology and UCD Conway Institute for Biomolecular and Biomedical Research, University College Dublin , Belfield, Dublin 4, Ireland.
authors.forename: Daniele
authors.initials: D
authors.lastname: Maiolo
authors.forename: Paolo
authors.initials: P
authors.lastname: Bergese
authors.forename: Eugene
authors.initials: E
authors.lastname: Mahon
authors.forename: Kenneth A
authors.initials: KA
authors.lastname: Dawson
authors.forename: Marco P
authors.initials: MP
authors.lastname: Monopoli
mesh_terms: D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D053758:Nanoparticles / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry*; D013501:Surface-Active Agents / Q000737:chemistry*
chemical_list: D011506:Proteins; D013501:Surface-Active Agents