title: Human beta defensin-4 and keratinocyte growth factor gene expression in cultured keratinocyte and fibroblasts of burned patients.
abstract: OBJECTIVE
  To evaluate KGF and human beta defensin-4 (HBD-4) levels produced by dermic fibroblasts and keratinocytes cultivated from burned patients' skin samples.
  
  
  METHODS
  Keratinocytes and fibroblasts of 10 patients (four major burns, four minor burns and two controls) were primarily cultivated according to standard methods. HBD-4 and KGF genes were analyzed by quantitative PCR.
  
  
  RESULTS
  In fibroblasts, KGF gene expression was 220±80 and 33.33±6.67 (M±SD; N=4), respectively for major and minor burn groups. In keratinocytes, KGF gene expression was 11.2±1.9 and 3.45±0.37 (M±SD; N=4), respectively for major and minor burn groups. In fibroblasts, HBD-4 gene expression was 15.0±4.0 and 11.5±0.5 (M±SD; N=4), respectively for major and minor burn. In keratinocyte, HBD-4 gene expression was 0.0±0.0 and 13.4±4.8 (M±SD; N=4), respectively for major and minor burn.
  
  
  CONCLUSIONS
  KGF expression was increased in burn patient fibroblasts compared to control group. In keratinocytes culture, KGF suppression is inversely proportional to burn extension; it is active and increased in major burn but decreased in minor burn. HBD-4 expression was increased in fibroblasts and decreased in keratinocytes from all burned patients.
authors.affiliation: Department of Surgery, EPM, UNIFESP, Sao Paulo, SP, Brazil.
authors.forename: Silvana Aparecida Alves Corrêa de
authors.initials: SA
authors.lastname: Noronha
authors.affiliation: Department of Surgery, EPM, UNIFESP, Sao Paulo, SP, Brazil.
authors.forename: Samuel Marcos Ribeiro de
authors.initials: SM
authors.lastname: Noronha
authors.affiliation: EPM, UNIFESP, Sao Paulo, SP, Brazil.
authors.forename: Larissa Elias
authors.initials: LE
authors.lastname: Lanziani
authors.affiliation: EPM, UNIFESP, Sao Paulo, SP, Brazil.
authors.forename: Michele Zampieri
authors.initials: MZ
authors.lastname: Ipolito
authors.affiliation: Department of Surgery, EPM, UNIFESP, Sao Paulo, SP, Brazil.
authors.forename: Lydia Masako
authors.initials: LM
authors.lastname: Ferreira
authors.affiliation: Department of Surgery, Paulista School of Medicine, Federal University of Sao Paulo, Brazil.
authors.forename: Alfredo
authors.initials: A
authors.lastname: Gragnani
mesh_terms: D002056:Burns / Q000235:genetics*; D002478:Cells, Cultured; D005260:Female; D051523:Fibroblast Growth Factor 7 / Q000032:analysis* / Q000235:genetics; D005347:Fibroblasts / Q000378:metabolism*; D015870:Gene Expression; D006801:Humans; D015603:Keratinocytes / Q000378:metabolism*; D008297:Male; D016133:Polymerase Chain Reaction; D012313:RNA / Q000302:isolation & purification; D012867:Skin / Q000166:cytology / Q000293:injuries; D055815:Young Adult; D023083:beta-Defensins / Q000235:genetics* / Q000378:metabolism
chemical_list: C106641:DEFB4A protein, human; D023083:beta-Defensins; D051523:Fibroblast Growth Factor 7; D012313:RNA