title: A solid-phase affinity labeling method for target-selective isolation and modification of proteins.
abstract: Solid-phase affinity labeling of a target protein, peanut agglutinin (PNA), with the specifically designed chemical tool 1 selectively and effectively furnished the labeled PNA. Furthermore, this method was applicable to native human carbonic anhydrase II in red blood cell lysate using the chemical tool 2 without the need for tedious manipulations.
authors.affiliation: Department of Applied Chemistry, Faculty of Science and Technology, Keio University, 3-14-1 Hiyoshi, Kohoku-ku, Yokohama 223-8522, Japan. dtak@applc.keio.ac.jp toshima@applc.keio.ac.jp.
authors.forename: Daichi
authors.initials: D
authors.lastname: Kuwahara
authors.forename: Takahiro
authors.initials: T
authors.lastname: Hasumi
authors.forename: Hajime
authors.initials: H
authors.lastname: Kaneko
authors.forename: Madoka
authors.initials: M
authors.lastname: Unno
authors.forename: Daisuke
authors.initials: D
authors.lastname: Takahashi
authors.forename: Kazunobu
authors.initials: K
authors.lastname: Toshima
mesh_terms: D000345:Affinity Labels / Q000737:chemistry*; D000818:Animals; D024402:Carbonic Anhydrase II / Q000302:isolation & purification; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D019887:Peanut Agglutinin / Q000302:isolation & purification; D011506:Proteins / Q000302:isolation & purification*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D000345:Affinity Labels; D019887:Peanut Agglutinin; D011506:Proteins; D024402:Carbonic Anhydrase II