title: Shuanghuanglian injection downregulates nuclear factor-kappa B expression in mice with viral encephalitis.
abstract: A mouse model of viral encephalitis was induced by intracranial injection of a Coxsackie virus B3 suspension. Quantitative real-time reverse transcription-PCR and western blot assay were applied to detect mRNA and protein expression of intelectin-2 and nuclear factor-kappa B in the viral encephalitis and control groups. Nuclear factor-kappa B and intelectin-2 mRNA and protein expression were significantly increased in mice with viral encephalitis. After intraperitoneal injection of Shuanghuanglian at a dose of 1.5 mg/kg for 5 successive days, intelectin-2 and nuclear factor-kappa B protein and mRNA expression were significantly decreased. To elucidate the relationship between intelectin-2 and nuclear factor-kappa B, mice with viral encephalitis were administered an intracerebral injection of 107 pfu recombinant lentivirus expressing intelectin shRNA. Both protein and mRNA levels of intelectin and nuclear factor-kappa B in brain tissue of mice were significantly decreased. Experimental findings suggest that Shuanghuanglian injection may downregulate nuclear factor-kappa B production via suppression of intelectin production, thus inhibiting inflammation associated with viral encephalitis.
authors.affiliation: Department of Neurology, Xi'an Center Hospital, Xi'an 710003, Shaanxi Province, China.
authors.forename: Naibing
authors.initials: N
authors.lastname: Gu
authors.affiliation: Department of Neurology, Xi'an Center Hospital, Xi'an 710003, Shaanxi Province, China.
authors.forename: Ye
authors.initials: Y
authors.lastname: Tian
authors.affiliation: Department of Neurology, Xi'an Center Hospital, Xi'an 710003, Shaanxi Province, China.
authors.forename: Zhengli
authors.initials: Z
authors.lastname: Di
authors.affiliation: Department of Neurology, Xi'an Center Hospital, Xi'an 710003, Shaanxi Province, China.
authors.forename: Caiping
authors.initials: C
authors.lastname: Han
authors.affiliation: Department of Neurology, Xi'an Center Hospital, Xi'an 710003, Shaanxi Province, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Lei
authors.affiliation: Department of Neurology, Xi'an Center Hospital, Xi'an 710003, Shaanxi Province, China.
authors.forename: Gejuan
authors.initials: G
authors.lastname: Zhang
keywords: Shuanghuanglian injection; intelectin; lentivirus; mice; nervous system disease; neural regeneration; nuclear factor-kappa B; short hairpin RNA; traditional Chinese medicine; viral encephalitis