title: Bioconversion of rebaudioside I from rebaudioside A.
abstract: To supply the increasing demand of natural high potency sweeteners to reduce the calories in food and beverages, we have looked to steviol glycosides. In this work we report the bioconversion of rebaudioside A to rebaudioside I using a glucosyltransferase enzyme. This bioconversion reaction adds one sugar unit with a 1→3 linkage. We utilized 1D and 2D NMR spectroscopy (1H, 13C, COSY, HSQC-DEPT, HMBC, 1D TOCSY and NOESY) and mass spectral data to fully characterize rebaudioside I.
authors.affiliation: The Coca-Cola Company, Atlanta, GA 30313, USA. iprakash@coca-cola.com.
authors.forename: Indra
authors.initials: I
authors.lastname: Prakash
authors.affiliation: The Coca-Cola Company, Atlanta, GA 30313, USA. cbunders@coca-cola.com.
authors.forename: Cynthia
authors.initials: C
authors.lastname: Bunders
authors.affiliation: AMRI-Albany, Analytical Development, Albany, NY 12212, USA. Krishna.Devkota@amriglobal.com.
authors.forename: Krishna P
authors.initials: KP
authors.lastname: Devkota
authors.affiliation: AMRI-Albany, Analytical Development, Albany, NY 12212, USA. Romila.Charan@amriglobal.com.
authors.forename: Romila D
authors.initials: RD
authors.lastname: Charan
authors.affiliation: AMRI-Albany, Analytical Development, Albany, NY 12212, USA. Catherine.Ramirez@amriglobal.com.
authors.forename: Catherine
authors.initials: C
authors.lastname: Ramirez
authors.affiliation: AMRI-Albany, Analytical Development, Albany, NY 12212, USA. Tara.Snyder@amriglobal.com.
authors.forename: Tara M
authors.initials: TM
authors.lastname: Snyder
authors.affiliation: AMRI-Albany, Analytical Development, Albany, NY 12212, USA. Christopher.Priedemann@amriglobal.com.
authors.forename: Christopher
authors.initials: C
authors.lastname: Priedemann
authors.affiliation: PureCircle Limited, Lengkuk Teknologi, Bandar Enstek 71760, Malaysia. avetik@purecircle.com.
authors.forename: Avetik
authors.initials: A
authors.lastname: Markosyan
authors.affiliation: Libragen SA, 3 Rue des Satellites, Toulouse 31400, France. c.jarrin@libragen.com.
authors.forename: Cyrille
authors.initials: C
authors.lastname: Jarrin
authors.affiliation: Libragen SA, 3 Rue des Satellites, Toulouse 31400, France. r.terhalle@libragen.com.
authors.forename: Robert Ter
authors.initials: RT
authors.lastname: Halle
mesh_terms: D001628:Beverages; D045786:Diterpenes, Kaurane / Q000378:metabolism*; D005502:Food; D005960:Glucosides / Q000378:metabolism; D005964:Glucosyltransferases / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D013058:Mass Spectrometry / Q000379:methods; D013549:Sweetening Agents / Q000378:metabolism
chemical_list: D045786:Diterpenes, Kaurane; D005960:Glucosides; D013549:Sweetening Agents; C025474:rebaudioside A; D005964:Glucosyltransferases