title: Characterization of ESBL-producing Escherichia coli and Klebsiella pneumoniae from bloodstream infections in three hospitals in Bucharest, Romania: a preliminary study.
abstract: BACKGROUND
  Our objective was to describe the distribution of extended-spectrum beta-lactamase (ESBL) genes found in Escherichia coli and Klebsiella pneumoniae isolates from hospital-acquired or community-onset bloodstream infections (BSIs) in Bucharest, Romania.
  
  
  METHODS
  E. coli and K. pneumoniae isolates, resistant to at least one antimicrobial drug family, were analyzed for genes encoding ESBL by PCR and sequencing. The E. coli isolates were screened by an ST131 clone allele-specific PCR for the pabB gene.
  
  
  RESULTS
  ESBL genes were found in 30 (35%) of 85 drug-resistant E. coli and 19 (70%) of 27 drug-resistant K. pneumoniae. Twenty-nine (97%) E. coli carried blaCTX-M-15. Nineteen (63%) E. coli carrying an ESBL gene belonged to the ST131 clonal group. The blaCTX-M-15 gene was found in 15 (79%) and blaSHV-12 in 12 (63%) K. pneumoniae isolates.
  
  
  CONCLUSIONS
  In Bucharest CTX-M15 was the most frequently encountered ESBL in both E. coli and K. pneumoniae BSI isolates regardless of the setting of infections.
authors.affiliation: From the National Institute for Infectious Diseases "Prof. Dr. Matei Bals" , Bucharest , Romania.
authors.forename: Adriana
authors.initials: A
authors.lastname: Hristea
authors.forename: Ioana D
authors.initials: ID
authors.lastname: Olaru
authors.forename: Sheila
authors.initials: S
authors.lastname: Adams-Sapper
authors.forename: Lee W
authors.initials: LW
authors.lastname: Riley
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D016470:Bacteremia / Q000382:microbiology*; D001426:Bacterial Proteins / Q000235:genetics; D052978:Disk Diffusion Antimicrobial Tests; D004926:Escherichia coli / Q000187:drug effects / Q000201:enzymology / Q000235:genetics*; D004927:Escherichia coli Infections / Q000382:microbiology*; D006801:Humans; D007710:Klebsiella Infections / Q000382:microbiology*; D007711:Klebsiella pneumoniae / Q000187:drug effects / Q000201:enzymology / Q000235:genetics*; D012189:Retrospective Studies; D012383:Romania; D018440:beta-Lactam Resistance; D001618:beta-Lactamases / Q000235:genetics
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D001618:beta-Lactamases
keywords: CTX-M; ESBL; SHV; ST131; bloodstream infections