title: Transcriptional Profiling of a Cross-ProtectiveSalmonella entericaserovar Typhimurium UK-1damMutant Identifies a Set of Genes More Transcriptionally Active Compared to Wild-Type, and Stably Transcribed across Biologically Relevant Microenvironments.
abstract: Vaccination withSalmonella entericaserovar Typhimurium lacking DNA adenine methyltransferase confers cross-protective immunity against multipleSalmonellaserotypes. The mechanistic basis is thought to be associated with the de-repression of genes that are tightly regulated when transiting from one microenvironment to another. This de-repression provides a potential means for the production of a more highly expressed and stable antigenic repertoire capable of inducing cross-protective immune responses. To identify genes encoding proteins that may contribute to cross-protective immunity, we used aSalmonellaTyphimurium DNA adenine methyltransferase mutant strain (UK-1dammutant) derived from the parental UK-1 strain, and assessed the transcriptional profile of the UK-1dammutant and UK-1 strain grown under conditions that simulate the intestinal or endosomal microenvironments encountered during the infective process. As expected, the transcriptional profile of the UK-1dammutant identified a set of genes more transcriptionally active when compared directly to UK-1, and stably transcribed in biologically relevant culture conditions. Further, 22% of these genes were more highly transcribed in comparison to two other clinically-relevantSalmonellaserovars. The strategy employed here helps to identify potentially conserved proteins produced by the UK-1dammutant that stimulate and/or modulate the development of cross-protective immune responses toward multipleSalmonellaserotypes.
authors.affiliation: Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164, USA.
authors.forename: Claire B
authors.initials: CB
authors.lastname: Miller
authors.affiliation: Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164, USA ; Paul G. Allen School for Global Animal Health, Washington State University, Pullman, WA 99164, USA.
authors.forename: Sebastian Aguilar
authors.initials: SA
authors.lastname: Pierlé
authors.affiliation: Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164, USA ; Paul G. Allen School for Global Animal Health, Washington State University, Pullman, WA 99164, USA.
authors.forename: Kelly A
authors.initials: KA
authors.lastname: Brayton
authors.affiliation: Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164, USA.
authors.forename: Jennine N
authors.initials: JN
authors.lastname: Ochoa
authors.affiliation: Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164, USA ; Paul G. Allen School for Global Animal Health, Washington State University, Pullman, WA 99164, USA.
authors.forename: Devendra H
authors.initials: DH
authors.lastname: Shah
authors.affiliation: Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164, USA ; Paul G. Allen School for Global Animal Health, Washington State University, Pullman, WA 99164, USA ; Department of Biomedical Sciences and Pathobiology, Virginia Tech, Blacksburg, VA 24061, USA.
authors.forename: Kevin K
authors.initials: KK
authors.lastname: Lahmers
keywords: DNA adenine methyltransferase; Salmonella Typhimurium; bacteriophage; fimbriae; immunity; transcriptome