title: Bacterial metabarcoding by 16S rRNA gene ion torrent amplicon sequencing.
abstract: Ion Torrent is a next generation sequencing technology based on the detection of hydrogen ions produced during DNA chain elongation; this technology allows analyzing and characterizing genomes, genes, and species. Here, we describe an Ion Torrent procedure applied to the metagenomic analysis of 16S rRNA gene amplicons to study the bacterial diversity in food and environmental samples.
authors.affiliation: Italian National Agency for New technologies, Energy and Sustainable development, Roma, Italy.
authors.forename: Elio
authors.initials: E
authors.lastname: Fantini
authors.forename: Giulio
authors.initials: G
authors.lastname: Gianese
authors.forename: Giovanni
authors.initials: G
authors.lastname: Giuliano
authors.forename: Alessia
authors.initials: A
authors.lastname: Fiore
mesh_terms: D001419:Bacteria / Q000235:genetics*; D002874:Chromosome Mapping; D058893:DNA Barcoding, Taxonomic / Q000379:methods / Q000706:statistics & numerical data*; D017931:DNA Primers / Q000138:chemical synthesis / Q000737:chemistry; D004587:Electrophoresis, Agar Gel; D005798:Genes, Bacterial; D016680:Genome, Bacterial; D015698:Genomic Library; D059014:High-Throughput Nucleotide Sequencing / Q000295:instrumentation / Q000379:methods*; D056186:Metagenomics; D058977:Molecular Sequence Annotation; D016133:Polymerase Chain Reaction / Q000379:methods; D011522:Protons; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D017422:Sequence Analysis, DNA / Q000706:statistics & numerical data
chemical_list: D017931:DNA Primers; D011522:Protons; D012336:RNA, Ribosomal, 16S