title: Enhancing stress-resistance for efficient microbial biotransformations by synthetic biology.
abstract: Chemical conversions mediated by microorganisms, otherwise known as microbial biotransformations, are playing an increasingly important role within the biotechnology industry. Unfortunately, the growth and production of microorganisms are often hampered by a number of stressful conditions emanating from environment fluctuations and/or metabolic imbalances such as high temperature, high salt condition, strongly acidic solution, and presence of toxic metabolites. Therefore, exploring methods to improve the stress tolerance of host organisms could significantly improve the biotransformation process. With the help of synthetic biology, it is now becoming feasible to implement strategies to improve the stress-resistance of the existing hosts. This review summarizes synthetic biology efforts to enhance the efficiency of biotransformations by improving the robustness of microbes. Particular attention will be given to strategies at the cellular and the microbial community levels.
authors.affiliation: Department of Biological Engineering, School of Life Science, Beijing Institute of Technology , Beijing , China.
authors.forename: Haiyang
authors.initials: H
authors.lastname: Jia
authors.affiliation: Department of Biological Engineering, School of Life Science, Beijing Institute of Technology , Beijing , China.
authors.forename: Yanshuang
authors.initials: Y
authors.lastname: Fan
authors.affiliation: Department of Biological Engineering, School of Life Science, Beijing Institute of Technology , Beijing , China.
authors.forename: Xudong
authors.initials: X
authors.lastname: Feng
authors.affiliation: Department of Biological Engineering, School of Life Science, Beijing Institute of Technology , Beijing , China.
authors.forename: Chun
authors.initials: C
authors.lastname: Li
keywords: biotransformation; robustness; stress condition; stress-resistance; synthetic biology