title: Expression and significance of MOC-31 and calretinin in pleural fluid of patients with lung cancer.
abstract: BACKGROUND
  The cytologic assessment of pleural effusions to distinguish carcinoma cells from reactive mesothelial cells is particularly challenging. The aim of this study was to investigate the diagnostic value of monoclonal antibody (MOC-31) and calretinin in pleural fluid of patients with lung cancer to significantly improve the diagnostic accuracy.
  
  
  METHODS
  The expressions of MOC-31 and calretinin were detected by means of S-P immunocytochemical technique in pleural effusions of patients with lung cancer (n = 92) and in patients with benign lung disease (n = 70).
  
  
  RESULTS
  The positive rate of MOC-31 in pleural fluid was 90.2% (83/92) from patients with lung cancer and 2.9% (2/70) from patients with benign lung diseases, showing a significant difference (P < 0.01). Calretinin was expressed 87.1% (61/70) in benign lung diseases and 6.5% (6/92) in lung cancer, also showing a significant difference (P < 0.01). The optimal combination for assay was MOC-31 + calretinin: Sensitivity and specificity were 100 and 98.6%, respectively.
  
  
  CONCLUSIONS
  MOC-31 and calretinin are of important clinical value for diagnosing and differentially diagnosing the cancer cells in pleural fluid of patients with lung cancer.
authors.affiliation: Department of Pathology, Jilin Cancer Hospital, Changchun, 130012, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Lv
authors.affiliation: Department of Pathology, Jilin Cancer Hospital, Changchun, 130012, China.
authors.forename: Ji-Hong
authors.initials: JH
authors.lastname: Leng
authors.affiliation: Department of Pathology, Jilin Cancer Hospital, Changchun, 130012, China.
authors.forename: Yan-Yong
authors.initials: YY
authors.lastname: Hao
authors.affiliation: Department of Pathology, Jilin Cancer Hospital, Changchun, 130012, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Sun
authors.affiliation: Department of Pathology, Changchun Obstetrics Gynecology Hospital, Changchun, 130042, China.
authors.forename: Na
authors.initials: N
authors.lastname: Cha
authors.affiliation: Department of Pathology, The First Affiliated Hospital and College of Basic Medical Sciences, China Medical University, Shenyang, 110001, China.
authors.forename: Guang-Ping
authors.initials: GP
authors.lastname: Wu
mesh_terms: D000230:Adenocarcinoma / Q000175:diagnosis* / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000911:Antibodies, Monoclonal / Q000235:genetics* / Q000378:metabolism; D014408:Biomarkers, Tumor / Q000235:genetics* / Q000378:metabolism; D064032:Calbindin 2 / Q000235:genetics* / Q000378:metabolism; D005260:Female; D015870:Gene Expression; D006801:Humans; D007150:Immunohistochemistry; D008175:Lung Neoplasms / Q000175:diagnosis* / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D008297:Male; D008875:Middle Aged; D035422:Pleural Cavity / Q000378:metabolism / Q000473:pathology; D016066:Pleural Effusion, Malignant / Q000175:diagnosis* / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D012680:Sensitivity and Specificity
chemical_list: D000911:Antibodies, Monoclonal; D014408:Biomarkers, Tumor; C576764:CALB2 protein, human; D064032:Calbindin 2; C546130:MOC-31 monoclonal antibody, human
keywords: MOC-31; calretinin; immunocytochemistry; lung neoplasms; pleural fluid; tumor markers