title: Enhanced chondrogenesis with upregulation of PKR using a novel hydrostatic pressure bioreactor.
abstract: In this study, we developed a novel bioreactor to load hydrostatic pressure to promote chondrogenesis of prechondrogenic ATDC5 cells in as little as 3 days. Furthermore, we showed that loading hydrostatic pressure induced the upregulation of PKR, which is known to participate in mechanotransduction in various models.
authors.affiliation: a Department of Mechanical Engineering, Graduate School of Engineering , University of Tokyo , Tokyo , Japan.
authors.forename: Jeonghyun
authors.initials: J
authors.lastname: Kim
authors.forename: Kevin
authors.initials: K
authors.lastname: Montagne
authors.forename: Takashi
authors.initials: T
authors.lastname: Ushida
authors.forename: Katsuko
authors.initials: K
authors.lastname: Furukawa
mesh_terms: D000818:Animals; D019149:Bioreactors; D002460:Cell Line; D020219:Chondrogenesis / Q000235:genetics*; D006874:Hydrostatic Pressure; D040542:Mechanotransduction, Cellular; D051379:Mice; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D015854:Up-Regulation; D019892:eIF-2 Kinase / Q000235:genetics*
chemical_list: D012333:RNA, Messenger; D019892:eIF-2 Kinase
keywords: ATDC5; bioreactor; chondrogenesis; hydrostatic pressure; mechanotransduction