title: Re-emergence of circulatory foot-and-mouth disease virus serotypes Asia1 in Bangladesh and VP1 protein heterogeneity with vaccine strain IND 63/72.
abstract: Foot-and-mouth disease virus (FMDV) serotypes O, A and Asia1 are responsible for significant number of disease outbreaks in Bangladesh; however serotype Asia1 has not been reported in circulation since 1996. The present investigation reports the detection of serotype FMDV Asia1 from local farms in 2012 and 2013 outbreaks. The farms were located in Jessore and Gazipur districts, and one of these farms was under vaccine control programme. Phylogenetic analysis of the complete VP1 gene revealed that FMDV Asia1 is under genetic lineage C having close similarity to the Asia1 sequences of Indian origin. The circulatory genotype Asia1 showed VP1 protein sequence heterogeneity of eight amino acid substitutions within the G-H loop with the vaccine strain [IND 63/72 (AY304994)] used in vaccination programme. ELISA assay revealed that, of seven, only one local field serum sample (cattle vaccinated 38 days earlier) was positive at a titre level of >2.4 (log10) but failed to protect the cattle from infection occurred by the virus. This investigation focused that the eight amino acid substitution in VP1 protein at G-H loop of the locally circulated FMDV serotype Asia1 strain may be a reason for current vaccination failure.
authors.affiliation: Department of Microbiology, University of Dhaka, Dhaka, Bangladesh.
authors.forename: H
authors.initials: H
authors.lastname: Ullah
authors.affiliation: Department of Microbiology, University of Dhaka, Dhaka, Bangladesh.
authors.forename: M A
authors.initials: MA
authors.lastname: Siddique
authors.affiliation: Department of Microbiology, University of Dhaka, Dhaka, Bangladesh.
authors.forename: Md
authors.initials: M
authors.lastname: Al Amin
authors.affiliation: Department of Livestock Services, Central Disease Investigation Laboratory, Dhaka, Bangladesh.
authors.forename: B C
authors.initials: BC
authors.lastname: Das
authors.affiliation: Department of Microbiology, University of Dhaka, Dhaka, Bangladesh.
authors.forename: M
authors.initials: M
authors.lastname: Sultana
authors.affiliation: Department of Microbiology, University of Dhaka, Dhaka, Bangladesh.
authors.forename: M A
authors.initials: MA
authors.lastname: Hossain
mesh_terms: D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D000818:Animals; D001459:Bangladesh / Q000453:epidemiology; D001483:Base Sequence; D036022:Capsid Proteins / Q000737:chemistry / Q000235:genetics*; D002417:Cattle; D002418:Cattle Diseases / Q000453:epidemiology / Q000821:virology*; D005536:Foot-and-Mouth Disease / Q000453:epidemiology / Q000821:virology*; D029544:Foot-and-Mouth Disease Virus / Q000145:classification / Q000235:genetics* / Q000276:immunology*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010802:Phylogeny; D011487:Protein Conformation; D065288:Serogroup; D014765:Viral Vaccines
chemical_list: D036022:Capsid Proteins; C442501:VP1 protein, Foot-and-mouth disease virus; D014765:Viral Vaccines
keywords: Asia1; ELISA; FMDV; antigenic heterogeneity; vaccination failure