title: Osteocalcin/fibronectin-functionalized collagen matrices for bone tissue engineering.
abstract: Collagen is the most abundant protein found in the extracellular matrix and is widely used to build scaffolds for biomedical applications which are the result of its biocompatibility and biodegradability. In the present study, we constructed a rhOCN/FNIII9-10 fusion protein and rhOCN/FNIII9-10-functionalized collagen matrices and investigated the potential value for bone tissue engineering. In vitro studies carried out with preosteoblastic MC3T3-E1 cells showed that rhOCN/FNIII9-10 fusion protein promoted cell adhesion and the mRNA levels of osteogenic markers including osteocalcin, runt-related transcription factor 2, alkaline phosphatase (ALP), and collagen type I. In addition, rhOCN/FNIII9-10-functionalized collagen matrices showed significant induction of the ALP activity more than rhFNIII9-10-functionalized collagen matrices or collagen matrices alone. These results suggested that rhOCN/FNIII9-10-functionalized collagen matrices have potential for bone tissue engineering.
authors.affiliation: Department of Biochemistry, Inha University School of Medicine, Incheon, 400-712, Republic of Korea.
authors.forename: S G
authors.initials: SG
authors.lastname: Kim
authors.forename: D S
authors.initials: DS
authors.lastname: Lee
authors.forename: S
authors.initials: S
authors.lastname: Lee
authors.forename: J-H
authors.initials: JH
authors.lastname: Jang
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D001842:Bone and Bones / Q000187:drug effects / Q000502:physiology*; D002448:Cell Adhesion / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D003094:Collagen / Q000494:pharmacology*; D005353:Fibronectins / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D051379:Mice; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects; D015675:Osteocalcin / Q000494:pharmacology*; D010012:Osteogenesis / Q000187:drug effects; D011993:Recombinant Fusion Proteins / Q000494:pharmacology; D023822:Tissue Engineering / Q000379:methods*
chemical_list: D015415:Biomarkers; D005353:Fibronectins; D011993:Recombinant Fusion Proteins; D015675:Osteocalcin; D003094:Collagen; D000469:Alkaline Phosphatase
keywords: MC3T3-E1; bone tissue engineering; cell adhesion; collagen matrices; osteogenic differentiation; rhOCN/FNIII9-10