title: Elevated expression of NLRP1 and IPAF are related to oral pemphigus vulgaris pathogenesis.
abstract: Oral pemphigus vulgaris (PV) is a rare blistering disorder of the skin and mucous membranes in the mouth. Inflammasome serves as a molecular platform that mediates the autoactivation of caspase-1, which cleaves the pro-forms of IL-1β and IL-18 to active forms. Therefore, the main aim of this study was to evaluate the messenger RNA (mRNA) levels of NOD-like receptor-related protein (NLRP)1, NLRP3, and IPAF in the PBMCs of PV patients to determine their effect in PV pathogenesis. This study was designed as a cross-sectional study. We studied mRNA levels of three types of inflammasomes including NLRP1, NLRP3, and IPAF in 43 oral PV patients and 40 healthy controls by real-time PCR technique. Results were analyzed by SPSS software package version 18. Here, we showed that the mRNA levels of NLRP1 and IPAF in patients with active PV remarkably increased compared to those in healthy controls. However, the mRNA level of NLRP3 in PBMC of PV patients was similar to that of the control group. We showed important and emerging relationship of NLRP1 and IPAF mRNAs with PV disease progression. We hypothesize that NLRP1 and IPAF with cytokine activity of IL-1β are involved in the inflammation in PV patients.
authors.affiliation: Department of Orthodontics, School of Dentistry, Hamedan University of Medical Sciences, Hamedan, Iran.
authors.forename: Reza Mahvelati
authors.initials: RM
authors.lastname: Shamsabadi
authors.forename: Soroush
authors.initials: S
authors.lastname: Basafa
authors.forename: Reza
authors.initials: R
authors.lastname: Yarahmadi
authors.forename: Samaneh
authors.initials: S
authors.lastname: Goorani
authors.forename: Masood
authors.initials: M
authors.lastname: Khani
authors.forename: Maryam
authors.initials: M
authors.lastname: Kamarehei
authors.forename: Amir
authors.initials: A
authors.lastname: Hossein Kiani
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000096:biosynthesis* / Q000097:blood; D051017:Apoptosis Regulatory Proteins / Q000096:biosynthesis* / Q000097:blood; D015415:Biomarkers / Q000097:blood; D053468:CARD Signaling Adaptor Proteins / Q000096:biosynthesis* / Q000097:blood; D002135:Calcium-Binding Proteins / Q000096:biosynthesis* / Q000097:blood; D003430:Cross-Sectional Studies; D005786:Gene Expression Regulation; D006801:Humans; D053583:Interleukin-1beta / Q000097:blood; D000070576:NLR Proteins; D010392:Pemphigus / Q000097:blood* / Q000175:diagnosis
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D051017:Apoptosis Regulatory Proteins; D015415:Biomarkers; D053468:CARD Signaling Adaptor Proteins; D002135:Calcium-Binding Proteins; D053583:Interleukin-1beta; D000070576:NLR Proteins; C435692:NLRC4 protein, human; C427903:NLRP1 protein, human