title: Complete genome sequence of a novel human papillomavirus identified by metagenomic analysis from a child with diarrhea in China.
abstract: A highly divergent human papillomavirus, named HPV-CH2, was identified in fecal samples from children with diarrhea in China by 454 high-throughput sequencing. Here, we report the complete genome sequence and genetic organization of the virus. The full-length nucleotide sequence of HPV-CH2 shares the highest sequence similarity with HPV-156, with 72 % nucleotide sequence identity. The L1 gene of the HPV-CH2 shared <70 % nucleotide identity with previously reported HPVs, suggesting HPV-CH2 as a new type of papillomavirus. Phylogenetic analysis revealed that the HPV-CH2 belongs to the genus Gammapapillomavirus. No HPV-CH2 was detected by PCR in samples from children with both gastroenteritis and respiratory infection.
authors.affiliation: National Institute for Viral Disease Control and Prevention, China CDC, Beijing, 100052, China.
authors.forename: Jie-mei
authors.initials: JM
authors.lastname: Yu
authors.forename: Guoyan
authors.initials: G
authors.lastname: Zhao
authors.forename: Yuan-yun
authors.initials: YY
authors.lastname: Ao
authors.forename: Li-li
authors.initials: LL
authors.lastname: Li
authors.forename: David
authors.initials: D
authors.lastname: Wang
authors.forename: Zhao-jun
authors.initials: ZJ
authors.lastname: Duan
mesh_terms: D001483:Base Sequence; D002675:Child, Preschool; D002681:China; D004279:DNA, Viral / Q000235:genetics*; D003967:Diarrhea / Q000821:virology*; D005243:Feces / Q000821:virology; D052689:Gammapapillomavirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D014644:Genetic Variation; D016679:Genome, Viral / Q000235:genetics*; D006801:Humans; D007223:Infant; D056186:Metagenomics; D030361:Papillomavirus Infections / Q000821:virology; D010802:Phylogeny; D017422:Sequence Analysis, DNA
chemical_list: D004279:DNA, Viral