title: The defect of SFRP2 modulates an influx of extracellular calcium in B lymphocytes.
abstract: BACKGROUND
  In the Wnt pathway, the secreted frizzled-related protein 2 (SFRP2) is thought to act as one of the several competitive inhibitors of Wnt. However, the precise role of SFRP2 is still poorly understood especially in B lymphocytes. Here, we investigated the function of SFRP2, comparing the SFRP2 defective as well as normal B lymphocytes in mice.
  
  
  RESULTS
  We demonstrated that calcium influx from extracellular to intracellular space in splenic B cells was clearly affected by the defect of SFRP2. In addition, the phosphorylation of phospholipase Cγ2 was observed to be reduced in SFRP2 defective splenic B cells with B cell receptor stimulation.
  
  
  CONCLUSIONS
  SFRP2 is suggested to modulate the influx from extracellular calcium in the B cell receptor signaling pathway.
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Tokuda
authors.forename: Masami
authors.initials: M
authors.lastname: Tanaka
authors.forename: Tomohito
authors.initials: T
authors.lastname: Yagi
authors.affiliation: Department of Genomic Medical Sciences, Kyoto Prefectural University of Medicine, 465 kajii-cho, Kawaramachi-Hirokoji, Kamigyo-ku, Kyoto 602-8566, Japan. tashiro@koto.kpu-m.ac.jp.
authors.forename: Kei
authors.initials: K
authors.lastname: Tashiro
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology / Q000378:metabolism*; D015153:Blotting, Western; D001854:Bone Marrow Cells / Q000166:cytology / Q000378:metabolism; D002118:Calcium / Q000378:metabolism*; D002454:Cell Differentiation; D004721:Endoplasmic Reticulum / Q000378:metabolism; D005110:Extracellular Space / Q000378:metabolism*; D008565:Membrane Proteins / Q000378:metabolism*; D008810:Mice, Inbred C57BL; D051966:Phospholipase C gamma / Q000378:metabolism; D010766:Phosphorylation; D051176:beta Catenin / Q000378:metabolism; D000094742:Secreted Frizzled-Related Proteins
chemical_list: D002118:Calcium; D008565:Membrane Proteins; D051966:Phospholipase C gamma; D051176:beta Catenin; C491628:Sfrp2 protein, mouse; D000094742:Secreted Frizzled-Related Proteins