title: Heterogeneous nano metal-organic framework fluorescence probe for highly selective and sensitive detection of hydrogen sulfide in living cells.
abstract: Hydrogen sulfide (H2S) has been regarded as the third important gaseous signaling molecule involved in human physiological and pathological processes. Due to the high reactive and diffusible properties of H2S, real-time detection of H2S fluctuations in living biological specimens is crucial. Here, we present a Cu(II)-metalated 3D porous nanoscale metal-organic framework (nano-MOF) {CuL[AlOH]2}n (PAC; H6L = meso-tetrakis(4-carboxylphenyl)porphyrin) and successfully employ this nano-MOF as a novel heterogeneous fluorescence probe for H2S detection. As far as we know, nano-MOFs have never been used as selective fluorescence probes for H2S detection. On the basis of the advantages of nano-MOF materials, this biocompatible nano-MOF probe exhibits rapid response, excellent selectivity, and hypotoxicity in in situ detection of H2S and represents the most sensitive fluorescence probe for selective H2S detection under physiological pH. In addition, confocal imaging was achieved successfully in living cells.
authors.affiliation: College of Chemistry, Chemical Engineering and Materials Science, Collaborative Innovation Center of Functionalized Probes for Chemical Imaging, Key Laboratory of Molecular and Nano Probes, Ministry of Education, Shangdong Provincial Key Laboratory of Clean Production of Fine Chemicals, Shandong Normal University , Jinan 250014, People's Republic of China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Ma
authors.forename: Hao
authors.initials: H
authors.lastname: Su
authors.forename: Xuan
authors.initials: X
authors.lastname: Kuang
authors.forename: Xiangyuan
authors.initials: X
authors.lastname: Li
authors.forename: Tingting
authors.initials: T
authors.lastname: Zhang
authors.forename: Bo
authors.initials: B
authors.lastname: Tang
mesh_terms: D002470:Cell Survival; D005456:Fluorescent Dyes / Q000737:chemistry*; D056945:Hep G2 Cells; D006801:Humans; D006862:Hydrogen Sulfide / Q000032:analysis*; D049329:Nanostructures / Q000737:chemistry*; D009942:Organometallic Compounds / Q000138:chemical synthesis / Q000737:chemistry*; D014407:Tumor Cells, Cultured
chemical_list: D005456:Fluorescent Dyes; D009942:Organometallic Compounds; D006862:Hydrogen Sulfide