title: A self-powered one-touch blood extraction system: a novel polymer-capped hollow microneedle integrated with a pre-vacuum actuator.
abstract: Blood is the gold standard sample medium that can provide a wide variety of useful biological information for the diagnosis of various diseases. For portable point-of-care diagnosis, blood extraction systems have attracted attention as easier, safer, and more rapid methods of collecting small blood volumes. In this paper, we introduce a novel self-powered one-touch blood extraction system created by assembling a smart polymer-capped hollow microneedle in a pre-vacuum polydimethylsiloxane actuator. The optimized hollow microneedle was precisely fabricated by drawing lithography for minimally invasive blood extraction, with a length of 1800 μm, an inner diameter of 60 μm, an outer diameter of 130 μm, and a bevel angle of 15°. The system utilizes only a single step for operation; a finger press activates the blood sampling process based on the negative pressure-driven force built into the pre-vacuum activated actuator. A sufficient volume of blood (31.3 ± 2.0 μl) was successfully extracted from a rabbit for evaluation using a micro total analysis system. The entire system was made of low-cost and disposable materials to achieve easy operation with a miniature structure and to meet the challenging requirements for single-use application in a point-of-care system without the use of any external power equipment.
authors.affiliation: Department of Biotechnology, Yonsei University, 50 Yonsei-ro, Seodaemun-gu, Seoul, Korea. hijung@yonsei.ac.kr.
authors.forename: Cheng Guo
authors.initials: CG
authors.lastname: Li
authors.forename: Manita
authors.initials: M
authors.lastname: Dangol
authors.forename: Chang Yeol
authors.initials: CY
authors.lastname: Lee
authors.forename: Mingyu
authors.initials: M
authors.lastname: Jang
authors.forename: Hyungil
authors.initials: H
authors.lastname: Jung
mesh_terms: D000818:Animals; D001800:Blood Specimen Collection / Q000295:instrumentation / Q000379:methods*; D004129:Dimethylpolysiloxanes / Q000737:chemistry; D009339:Needles; D019095:Point-of-Care Systems; D011108:Polymers / Q000737:chemistry*; D011817:Rabbits; D013499:Surface Properties; D014618:Vacuum
chemical_list: D004129:Dimethylpolysiloxanes; D011108:Polymers; C013830:baysilon