title: Novosphingobium and its potential role in chronic obstructive pulmonary diseases: insights from microbiome studies.
abstract: Bacterial infection of lung airways underlies some of the main complications of COPD, significantly impacting disease progression and outcome. Colonization by bacteria may further synergize, amplify, or trigger pathways of tissue damage started by cigarette smoke, contributing to the characteristic airway inflammation and alveolar destruction of COPD. We sought to elucidate the presence and types of lung bacterial populations in different stages of COPD, aimed at revealing important insights into the pathobiology of the disease. Sequencing of the bacterial small subunit ribosomal RNA gene in 55 well-characterized clinical lung samples, revealed the presence of Novosphingobium spp. (>2% abundance) in lungs of patients with GOLD 3-GOLD 4 COPD, cystic fibrosis and a subset of control individuals. Novosphingobium-specific quantitative PCR was concordant with the sequence data and high levels of Novosphingobium spp. were quantifiable in advanced COPD, but not from other disease stages. Using a mouse model of subacute lung injury due to inhalation of cigarette smoke, bronchoalveolar lavage neutrophil and macrophage counts were significantly higher in mice challenged intratracheally with N. panipatense compared to control mice (p<0.01). Frequencies of neutrophils and macrophages in lung tissue were increased in mice challenged with N. panipatense at room air compared to controls. However, we did not observe an interaction between N. panipatense and subacute cigarette smoke exposure in the mouse. In conclusion, Novosphingobium spp. are present in more severe COPD disease, and increase inflammation in a mouse model of smoke exposure.
authors.affiliation: University of Colorado School of Medicine, Program in Translational Lung Research, Division of Pulmonary Sciences and Critical Care Medicine, Anschutz Medical Campus, Aurora, Colorado, United States of America.
authors.forename: Alleluiah
authors.initials: A
authors.lastname: Rutebemberwa
authors.affiliation: University of Colorado - School of Medicine, Department of Pediatrics, Pulmonary Medicine, Mucosal and Vaccine Research Colorado, Microbiome Research Colorado, Children's Hospital Colorado, Aurora, Colorado, United States of America.
authors.forename: Mark J
authors.initials: MJ
authors.lastname: Stevens
authors.affiliation: University of Colorado School of Medicine, Program in Translational Lung Research, Division of Pulmonary Sciences and Critical Care Medicine, Anschutz Medical Campus, Aurora, Colorado, United States of America.
authors.forename: Mario J
authors.initials: MJ
authors.lastname: Perez
authors.affiliation: University of Colorado School of Medicine, Program in Translational Lung Research, Division of Pulmonary Sciences and Critical Care Medicine, Anschutz Medical Campus, Aurora, Colorado, United States of America.
authors.forename: Lynelle P
authors.initials: LP
authors.lastname: Smith
authors.affiliation: University of Colorado School of Medicine, Program in Translational Lung Research, Division of Pulmonary Sciences and Critical Care Medicine, Anschutz Medical Campus, Aurora, Colorado, United States of America.
authors.forename: Linda
authors.initials: L
authors.lastname: Sanders
authors.affiliation: National Jewish Health, Department of Medicine and University of Colorado School of Medicine, Division of Pulmonary Sciences and Critical Care Medicine, Anschutz Medical Campus, Aurora, Colorado, United States of America.
authors.forename: Gregory
authors.initials: G
authors.lastname: Cosgrove
authors.affiliation: Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder, Colorado, United States of America.
authors.forename: Charles E
authors.initials: CE
authors.lastname: Robertson
authors.affiliation: University of Colorado School of Medicine, Program in Translational Lung Research, Division of Pulmonary Sciences and Critical Care Medicine, Anschutz Medical Campus, Aurora, Colorado, United States of America.
authors.forename: Rubin M
authors.initials: RM
authors.lastname: Tuder
authors.affiliation: University of Colorado - School of Medicine, Department of Pediatrics, Pulmonary Medicine, Mucosal and Vaccine Research Colorado, Microbiome Research Colorado, Children's Hospital Colorado, Aurora, Colorado, United States of America.
authors.forename: J Kirk
authors.initials: JK
authors.lastname: Harris
mesh_terms: D000328:Adult; D000368:Aged; D020561:Alphaproteobacteria / Q000302:isolation & purification / Q000472:pathogenicity*; D000818:Animals; D018893:Bronchoalveolar Lavage; D002469:Cell Separation; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007249:Inflammation; D008168:Lung / Q000378:metabolism / Q000382:microbiology / Q000503:physiopathology; D008264:Macrophages / Q000382:microbiology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D064307:Microbiota; D008875:Middle Aged; D009504:Neutrophils / Q000382:microbiology; D016133:Polymerase Chain Reaction; D029424:Pulmonary Disease, Chronic Obstructive / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D012906:Smoke; D012907:Smoking / Q000009:adverse effects
chemical_list: D012906:Smoke