title: Phosvitin phosphorus is involved in chicken embryo bone formation through dephosphorylation.
abstract: The aim of this study was to investigate the role of phosvitin in bone formation in chicken embryos. The yolk P content, P/N ratio and secondary structure of phosvitin, alkaline phosphatase (ALP) activity of the tibia, and body length were determined during incubation. A high correlation was found between the phosphate group content of phosvitin and both secondary structure and bone metabolism (ALP activity in the tibia, body length). The ALP activity and body length growth slightly lagged behind changes in the P/N ratio and the secondary structure of phosvitin. The phosphate content of phosvitin decreased, the γ-random coil and β-turn gradually transformed into α-helixes, and the secondary structure of protein tended to become more orderly; these changes mainly occurred on d 13 to 16. Bone formation of the chicken embryos occurred primarily on d 14 to 18, whereas ALP activity and body length growth increased substantially. The results indicate that phosvitin P is involved in chicken embryo bone formation through dephosphorylation.
authors.affiliation: National Research and Development Center for Egg Processing, College of Food Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, PR China.
authors.forename: Chunyan
authors.initials: C
authors.lastname: Li
authors.affiliation: National Research and Development Center for Egg Processing, College of Food Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, PR China.
authors.forename: Fang
authors.initials: F
authors.lastname: Geng
authors.affiliation: National Research and Development Center for Egg Processing, College of Food Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, PR China.
authors.forename: Xi
authors.initials: X
authors.lastname: Huang
authors.affiliation: National Research and Development Center for Egg Processing, College of Food Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, PR China mameihuhn@yahoo.com.cn.
authors.forename: Meihu
authors.initials: M
authors.lastname: Ma
authors.affiliation: National Research and Development Center for Egg Processing, College of Food Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, PR China.
authors.forename: Xiaowei
authors.initials: X
authors.lastname: Zhang
mesh_terms: D000818:Animals; D001846:Bone Development / Q000187:drug effects / Q000502:physiology*; D002642:Chick Embryo; D004530:Egg Yolk / Q000737:chemistry / Q000502:physiology; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D009584:Nitrogen / Q000737:chemistry / Q000378:metabolism; D010758:Phosphorus / Q000378:metabolism*; D010766:Phosphorylation; D010774:Phosvitin / Q000378:metabolism*; D017433:Protein Structure, Secondary; D013997:Time Factors
chemical_list: D010758:Phosphorus; D010774:Phosvitin; D009584:Nitrogen
keywords: bone formation; chicken embryo development; dephosphorylation; phosvitin