title: Innate and adaptive immunity gene expression of human keratinocytes cultured of severe burn injury.
abstract: OBJECTIVE
  Evaluate the expression profile of genes related to Innate and Adaptive Immune System (IAIS) of human Primary Epidermal keratinocytes (hPEKP) of patients with severe burns.
  
  
  METHODS
  After obtaining viable fragments of skin with and without burning, culture hKEP was initiated by the enzymatic method using Dispase (Sigma-Aldrich). These cells were treated with Trizol(r) (Life Technologies) for extraction of total RNA. This was quantified and analyzed for purity for obtaining cDNA for the analysis of gene expression using specific IAIS PCR Arrays plates (SA Biosciences).
  
  
  RESULTS
  After the analysis of gene expression we found that 63% of these genes were differentially expressed, of which 77% were repressed and 23% were hyper-regulated. Among these, the following genes (fold increase or decrease): IL8 (41), IL6 (32), TNF (-92), HLA-E (-86), LYS (-74), CCR6 (- 73), CD86 (-41) and HLA-A (-35).
  
  
  CONCLUSIONS
  This study contributes to the understanding of the molecular mechanisms underlying wound infection caused by the burn. Furthermore, it may provide new strategies to restore normal expression of these genes and thereby change the healing process and improve clinical outcome.
authors.affiliation: Department of Surgery, EPM, UNIFESP, Sao Paulo, SP, Brazil.
authors.forename: Silvana Aparecida Alves Corrêa de
authors.initials: SA
authors.lastname: Noronha
authors.affiliation: Department of Surgery, EPM, UNIFESP, Sao Paulo, SP, Brazil.
authors.forename: Samuel Marcos Ribeiro de
authors.initials: SM
authors.lastname: Noronha
authors.affiliation: EPM, UNIFESP, Sao Paulo, SP, Brazil.
authors.forename: Larissa Elias
authors.initials: LE
authors.lastname: Lanziani
authors.affiliation: UNIFESP, Brazil.
authors.forename: Lydia Masako
authors.initials: LM
authors.lastname: Ferreira
authors.affiliation: Department of Surgery, Paulista School of Medicine, Federal University of Sao Paulo, Brazil.
authors.forename: Alfredo
authors.initials: A
authors.lastname: Gragnani
mesh_terms: D056704:Adaptive Immunity / Q000235:genetics* / Q000276:immunology; D000328:Adult; D002056:Burns / Q000235:genetics* / Q000276:immunology; D002478:Cells, Cultured; D005260:Female; D015870:Gene Expression; D006801:Humans; D007113:Immunity, Innate / Q000235:genetics*; D015603:Keratinocytes / Q000166:cytology* / Q000276:immunology; D008297:Male; D016133:Polymerase Chain Reaction; D012107:Research Design; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis / Q000235:genetics; D014945:Wound Healing / Q000235:genetics
chemical_list: D014409:Tumor Necrosis Factor-alpha