title: Correlative multicolor 3D SIM and STORM microscopy.
abstract: Within the last decade, super-resolution methods that surpass the diffraction limit of light microscopy have provided invaluable insight into a variety of biological questions. Each of these approaches has inherent advantages and limitations, such that their combination is a powerful means to transform them into versatile tools for the life sciences. Here, we report the development of a combined SIM and STORM setup that maintains the optimal resolution of both methods and which is coupled to image registration to localize biological structures in 3D using multicolor labeling. We utilized this workflow to determine the localization of Bld12p/CrSAS-6 in purified basal bodies of Chlamydomonas reinhardtii with utmost precision, demonstrating its usefulness for accurate molecular mapping in 3D.
authors.affiliation: Swiss Institute for Experimental Cancer Research (ISREC), School of Life Sciences, Swiss Federal Institute of Technology (EPFL), Lausanne, Switzerland ; These authors contributed equally to this work.
authors.forename: Virginie
authors.initials: V
authors.lastname: Hamel
authors.affiliation: Swiss Institute for Experimental Cancer Research (ISREC), School of Life Sciences, Swiss Federal Institute of Technology (EPFL), Lausanne, Switzerland ; These authors contributed equally to this work.
authors.forename: Paul
authors.initials: P
authors.lastname: Guichard
authors.affiliation: Bioimaging and Optics platform (BIOP), School of Life Sciences, Swiss Federal Institute of Technology (EPFL), Lausanne, Switzerland ; These authors contributed equally to this work.
authors.forename: Mathias
authors.initials: M
authors.lastname: Fournier
authors.affiliation: Bioimaging and Optics platform (BIOP), School of Life Sciences, Swiss Federal Institute of Technology (EPFL), Lausanne, Switzerland.
authors.forename: Romain
authors.initials: R
authors.lastname: Guiet
authors.affiliation: Swiss Institute for Experimental Cancer Research (ISREC), School of Life Sciences, Swiss Federal Institute of Technology (EPFL), Lausanne, Switzerland.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Flückiger
authors.affiliation: Bioimaging and Optics platform (BIOP), School of Life Sciences, Swiss Federal Institute of Technology (EPFL), Lausanne, Switzerland.
authors.forename: Arne
authors.initials: A
authors.lastname: Seitz
authors.affiliation: Swiss Institute for Experimental Cancer Research (ISREC), School of Life Sciences, Swiss Federal Institute of Technology (EPFL), Lausanne, Switzerland.
authors.forename: Pierre
authors.initials: P
authors.lastname: Gönczy
keywords: (100.6640) Superresolution; (170.3880) Medical and biological imaging; (180.0180) Microscopy; (180.2520) Fluorescence microscopy; (180.6900) Three-dimensional microscopy