title: In vitro activity of glucosinolates and their degradation products against brassica-pathogenic bacteria and fungi.
abstract: Glucosinolates (GSLs) are secondary metabolites found in Brassica vegetables that confer on them resistance against pests and diseases. Both GSLs and glucosinolate hydrolysis products (GHPs) have shown positive effects in reducing soil pathogens. Information about their in vitro biocide effects is scarce, but previous studies have shown sinigrin GSLs and their associated allyl isothiocyanate (AITC) to be soil biocides. The objective of this work was to evaluate the biocide effects of 17 GSLs and GHPs and of leaf methanolic extracts of different GSL-enriched Brassica crops on suppressing in vitro growth of two bacterial (Xanthomonas campestris pv. campestris and Pseudomonas syringae pv. maculicola) and two fungal (Alternaria brassicae and Sclerotinia scletoriorum) Brassica pathogens. GSLs, GHPs, and methanolic leaf extracts inhibited the development of the pathogens tested compared to the control, and the effect was dose dependent. Furthermore, the biocide effects of the different compounds studied were dependent on the species and race of the pathogen. These results indicate that GSLs and their GHPs, as well as extracts of different Brassica species, have potential to inhibit pathogen growth and offer new opportunities to study the use of Brassica crops in biofumigation for the control of multiple diseases.
authors.affiliation: Group of Genetics, Breeding and Biochemistry of Brassicas, Misión Biológica de Galicia (MBG-CSIC), Pontevedra, Spain.
authors.forename: T
authors.initials: T
authors.lastname: Sotelo
authors.affiliation: Department of Ecology, Faculty of Biology, University of Santiago de Compostela, Santiago de Compostela, Spain.
authors.forename: M
authors.initials: M
authors.lastname: Lema
authors.affiliation: Group of Genetics, Breeding and Biochemistry of Brassicas, Misión Biológica de Galicia (MBG-CSIC), Pontevedra, Spain.
authors.forename: P
authors.initials: P
authors.lastname: Soengas
authors.affiliation: Group of Genetics, Breeding and Biochemistry of Brassicas, Misión Biológica de Galicia (MBG-CSIC), Pontevedra, Spain.
authors.forename: M E
authors.initials: ME
authors.lastname: Cartea
authors.affiliation: Group of Genetics, Breeding and Biochemistry of Brassicas, Misión Biológica de Galicia (MBG-CSIC), Pontevedra, Spain velasco@mbg.csic.es.
authors.forename: P
authors.initials: P
authors.lastname: Velasco
mesh_terms: D000528:Alternaria / Q000187:drug effects; D000900:Anti-Bacterial Agents / Q000378:metabolism / Q000494:pharmacology*; D000935:Antifungal Agents / Q000378:metabolism / Q000494:pharmacology*; D001203:Ascomycota / Q000187:drug effects; D001937:Brassica / Q000737:chemistry* / Q000382:microbiology; D005961:Glucosinolates / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests; D010935:Plant Diseases / Q000382:microbiology; D010936:Plant Extracts / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology*; D044224:Pseudomonas syringae / Q000187:drug effects; D016959:Xanthomonas campestris / Q000187:drug effects
chemical_list: D000900:Anti-Bacterial Agents; D000935:Antifungal Agents; D005961:Glucosinolates; D010936:Plant Extracts