title: Pulsed field gel electrophoresis and genome size estimates.
abstract: Pulsed field gel electrophoresis (PFGE) is a quick and reliable procedure to resolve DNA molecules larger than 30 kb by applying an electric field that periodically changes direction. This technique can be used to estimate genome size of a microorganism, to reveal if a genome is circular or linear, to indicate the presence of megaplasmids, and to show if a strain contains only one or more chromosomes.
authors.affiliation: Department of Biological, Chemical and Pharmaceutical Sciences and Technologies, University of Palermo, Via delle Scienze, Ed. 16, Palermo, 90128, Italy, valeria.alduina@unipa.it.
authors.forename: Rosa
authors.initials: R
authors.lastname: Alduina
authors.forename: Annalisa
authors.initials: A
authors.lastname: Pisciotta
mesh_terms: D001105:Archaea / Q000235:genetics*; D001419:Bacteria / Q000235:genetics*; D001482:Base Composition; D019847:Chromosomes, Archaeal / Q000737:chemistry; D002876:Chromosomes, Bacterial / Q000737:chemistry; D019641:DNA, Archaeal / Q000737:chemistry / Q000235:genetics; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004587:Electrophoresis, Agar Gel; D016521:Electrophoresis, Gel, Pulsed-Field / Q000379:methods*; D059646:Genome Size; D020745:Genome, Archaeal; D016680:Genome, Bacterial; D020161:Physical Chromosome Mapping; D010957:Plasmids / Q000737:chemistry / Q000235:genetics
chemical_list: D019641:DNA, Archaeal; D004269:DNA, Bacterial