title: Monoclonal antibodies against G protein of spring viremia of carp virus.
abstract: Part of the G protein (3094-4170 bp) of spring viremia of carp virus (SVCV) was expressed in Escherichia coli and purified by dialysis in our study. Two clones of monoclonal antibodies (MAbs 1H11 and 4B8) against G protein were generated by fusion of mouse myeloma cell line SP2/0 and spleen lymphocytes from part of G protein (3094-4170 bp) immunized mice. The results of ELISA (enzyme-linked immunosorbent assay), IFA (indirect immunofluorescent assay), and Western blot assay further demonstrated the characterizations of the two MAbs. Both 1H11 and 4B8 were specific to SVCV G protein. Ten pairs of synthesized overlapping peptides were used to identify the epitope of the MAbs. The MAbs are useful in the development of SVCV diagnostic methods.
authors.affiliation: 1 Department of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University , Wuhan, Hubei, China .
authors.forename: Peixiao
authors.initials: P
authors.lastname: Luo
authors.forename: Xindi
authors.initials: X
authors.lastname: Ruan
authors.forename: Qi
authors.initials: Q
authors.lastname: Zhang
authors.forename: Zeming
authors.initials: Z
authors.lastname: Li
authors.forename: Min
authors.initials: M
authors.lastname: Wang
authors.forename: Xueqin
authors.initials: X
authors.lastname: Liu
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity / Q000276:immunology; D002347:Carps / Q000276:immunology* / Q000821:virology*; D002460:Cell Line; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D000939:Epitopes / Q000235:genetics / Q000276:immunology; D004926:Escherichia coli / Q000235:genetics; D019084:Fluorescent Antibody Technique, Indirect / Q000379:methods; D019204:GTP-Binding Proteins / Q000235:genetics / Q000276:immunology*; D008214:Lymphocytes / Q000276:immunology / Q000821:virology; D051379:Mice; D006435:Renal Dialysis / Q000379:methods; D013154:Spleen / Q000821:virology; D014766:Viremia / Q000276:immunology* / Q000821:virology*
chemical_list: D000911:Antibodies, Monoclonal; D000939:Epitopes; D019204:GTP-Binding Proteins