title: Identification of hydrogen peroxide-secreting cells by cytocompatible coating with a hydrogel membrane.
abstract: A method for identifying each cell secreting reactive oxygen species (ROS) is highly desirable to advance the understanding of the physiological and pathological processes attributed to extracellular ROS. Here, we first report a method for realizing this. The individual cells secreting hydrogen peroxide (H2O2), a common ROS, could be coated by a hydrogel membrane through a horseradish peroxidase-catalyzed reaction consuming H2O2 secreted from the cells themselves. This hydrogel membrane coating was proved to be cytocompatible. In addition, the hydrogel membrane made from an alginate derivative could be removed on demand without causing damage to the enclosed cells. These results demonstrated the feasibility of the proposed method to be an effective tool in cellular ROS studies.
authors.affiliation: Division of Chemical Engineering, Department of Materials Engineering Science, Graduate School of Engineering Science, Osaka University , Toyonaka, Osaka 560-8531, Japan.
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.forename: Shinji
authors.initials: S
authors.lastname: Sakai
authors.forename: Shogo
authors.initials: S
authors.lastname: Kawa
authors.forename: Masahito
authors.initials: M
authors.lastname: Taya
mesh_terms: D000818:Animals; D055162:Biocatalysis; D001672:Biocompatible Materials / Q000737:chemistry*; D002477:Cells / Q000378:metabolism*; D002478:Cells, Cultured; D005434:Flow Cytometry; D056945:Hep G2 Cells; D006735:Horseradish Peroxidase / Q000378:metabolism; D006801:Humans; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry*; D006861:Hydrogen Peroxide / Q000032:analysis* / Q000378:metabolism*; D051379:Mice
chemical_list: D001672:Biocompatible Materials; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D006861:Hydrogen Peroxide; D006735:Horseradish Peroxidase