title: Investigation of total phenolic, total flavonoid, antioxidant and allyl isothiocyanate content in the different organs of Wasabi japonica grown in an organic system.
abstract: BACKGROUND
  This study was carried out to investigate the total polyphenol (TP), total flavonoid (TF), antioxidative effect and allyl isothyocyanate (ITC) content in different organs of wasabi plant grown in an organic system.
  
  
  METHODS
  Invitro study of methanol and boiled water extracts of wasabi were conducted by analyzing the 1-1-diphenyl-2-picryl hydrozyl (DPPH) radial scavenging activity, metal chelating activity and total antioxidant capacity in a comparative manner.
  
  
  RESULTS
  The result revealed that methanol extract showed higher TP in flower (3644 mg TAE/100 g dw), leaf (3201 mg TAE/100 g dw) and fruit (3025 mg TAE/100 g dw) as compared to the boiled water extract. Similarly, TF content was also higher in methanol extracts of flower (1152 mg QE/100 g dw) and leaf (325 mg QE/100 g dw), however, the other parts showed ignorable value.
  
  
  RESULTS
  Results of antioxidant activity were found at different magnitude of potency. The methanol extract of different parts of wasabi exhibited higher activity in total antioxidant capacity and DPPH radical scavenging assay as compared to water extract. In metal chelating assay, the boiled water extracts of leaf showed higher (76.9%) activity, followed by fruit (68.8%) and flower (62.8%). Ally ITC detected by gas chromatography was present in all of the tissues of wasabi plant but the content was found to be varied in different tissues.
  
  
  CONCLUSIONS
  Overall, this study will allow consumers and processors to understand the possibility for medical application of wasabi plant by knowing the level of total polyphenol distribution, Ally ITC content and antioxidant property distributed in different parts and tissues.Key words: Allyl ITC, antioxidant, flavonoid, polyphenol, Wasabi japonica.
authors.affiliation: College of Biomedical Science, Department of Bio-health Technology, Kangwon National University, Chuncheon 200-701, Republic of S. Korea.
authors.forename: Seong Woo
authors.initials: SW
authors.lastname: Shin
authors.affiliation: College of Biomedical Science, Department of Bio-health Technology, Kangwon National University, Chuncheon 200-701, Republic of S. Korea.
authors.forename: Amal Kumar
authors.initials: AK
authors.lastname: Ghimeray
authors.affiliation: College of Biomedical Science, Department of Bio-health Technology, Kangwon National University, Chuncheon 200-701, Republic of S. Korea.
authors.forename: Cheol Ho
authors.initials: CH
authors.lastname: Park
mesh_terms: D000975:Antioxidants / Q000032:analysis*; D005419:Flavonoids / Q000032:analysis*; D035264:Flowers / Q000737:chemistry; D005638:Fruit / Q000737:chemistry; D017879:Isothiocyanates / Q000032:analysis*; D058871:Organic Agriculture; D010636:Phenols / Q000032:analysis*; D010936:Plant Extracts / Q000032:analysis*; D018515:Plant Leaves / Q000737:chemistry; D031228:Wasabia / Q000737:chemistry* / Q000254:growth & development
chemical_list: D000975:Antioxidants; D005419:Flavonoids; D017879:Isothiocyanates; D010636:Phenols; D010936:Plant Extracts; C004471:allyl isothiocyanate