title: Robust, flexible, and bioadhesive free-standing films for the co-delivery of antibiotics and growth factors.
abstract: Free-standing polymer films that adhere strongly to tissue and can codeliver multiple therapeutic agents in a controlled manner are useful as medical plasters. In this study, a bilayer polymer film comprising a drug reservoir layer and a supporting layer is fabricated by spin-coating poly(lactic-co-glycolic acid) (PLGA) on top of a layer-by-layer assembled film of poly(β-amino esters) (PAE), alginate sodium (ALG), and recombinant human basic fibroblast growth factor (bFGF). Apart from bFGF, the bilayer film can also load antibiotic drug ceftriaxone sodium (CTX) by a postdiffusion process. The PLGA supporting layer facilitates the direct peeling of the bilayer film from substrate to produce a robust and flexible free-standing film with excellent adhesion onto the human skin and porcine liver. The excellent adhesion of the bilayer film originates from the ALG component in the drug reservoir layer. CTX is quickly released by easily breaking its electrostatic interaction with the drug reservoir layer, whereas the sustained release of bFGF is due to the slow degradation of PAE component in the drug reservoir layer. Wounds can be synergetically treated by fast release of CTX to effectively eradicate invasive bacteria and by sustained release of bFGF to accelerate wound healing. Our results serve as a basis for designing multifunctional free-standing films with combination therapy for biomedical applications.
authors.affiliation: State Key Laboratory of Supramolecular Structure and Materials, International Joint Research Laboratory of Nano-Micro Architecture Chemistry (NMAC), College of Chemistry, Jilin University , Changchun, P. R. China 130012.
authors.forename: Dongdong
authors.initials: D
authors.lastname: Chen
authors.forename: Mingda
authors.initials: M
authors.lastname: Wu
authors.forename: Jie
authors.initials: J
authors.lastname: Chen
authors.forename: Chunqiu
authors.initials: C
authors.lastname: Zhang
authors.forename: Tiezheng
authors.initials: T
authors.lastname: Pan
authors.forename: Bing
authors.initials: B
authors.lastname: Zhang
authors.forename: Huayu
authors.initials: H
authors.lastname: Tian
authors.forename: Xuesi
authors.initials: X
authors.lastname: Chen
authors.forename: Junqi
authors.initials: J
authors.lastname: Sun
mesh_terms: D000464:Alginates / Q000737:chemistry / Q000494:pharmacology; D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology; D001412:Bacillus subtilis / Q000187:drug effects*; D002443:Ceftriaxone / Q000737:chemistry / Q000494:pharmacology; D002460:Cell Line; D004926:Escherichia coli / Q000254:growth & development*; D016222:Fibroblast Growth Factor 2 / Q000737:chemistry / Q000494:pharmacology; D020723:Glucuronic Acid / Q000737:chemistry / Q000494:pharmacology; D006603:Hexuronic Acids / Q000737:chemistry / Q000494:pharmacology; D006801:Humans; D019344:Lactic Acid / Q000737:chemistry / Q000494:pharmacology; D008567:Membranes, Artificial; D051379:Mice; D011100:Polyglycolic Acid / Q000737:chemistry / Q000494:pharmacology; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D013552:Swine; D014014:Tissue Adhesives / Q000737:chemistry / Q000494:pharmacology
chemical_list: D000464:Alginates; D000900:Anti-Bacterial Agents; D006603:Hexuronic Acids; D008567:Membranes, Artificial; D014014:Tissue Adhesives; D016222:Fibroblast Growth Factor 2; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D002443:Ceftriaxone; D020723:Glucuronic Acid