title: A haploid genetics toolbox for Arabidopsis thaliana.
abstract: Genetic analysis in haploids provides unconventional yet powerful advantages not available in diploid organisms. In Arabidopsis thaliana, haploids can be generated through seeds by crossing a wild-type strain to a transgenic strain with altered centromeres. Here we report the development of an improved haploid inducer (HI) strain, SeedGFP-HI, that aids selection of haploid seeds prior to germination. We also show that haploids can be used as a tool to accelerate a variety of genetic analyses, specifically pyramiding multiple mutant combinations, forward mutagenesis screens, scaling down a tetraploid to lower ploidy levels and swapping of nuclear and cytoplasmic genomes. Furthermore, the A. thaliana HI can be used to produce haploids from a related species A. suecica and generate homozygous mutant plants from strong maternal gametophyte lethal alleles, which is not possible via conventional diploid genetics. Taken together, our results demonstrate the utility and power of haploid genetics in A. thaliana.
authors.affiliation: 1] Department of Plant Biology, University of California, Davis, California 95616, USA [2] School of Biology, Indian Institute of Science Education and Research, IISER-Thiruvananthapuram, CET Campus, Thiruvananthapuram 695016, India.
authors.forename: Maruthachalam
authors.initials: M
authors.lastname: Ravi
authors.affiliation: 1] Department of Plant Biology, University of California, Davis, California 95616, USA [2] Genome Center, University of California, Davis, California 95616, USA.
authors.forename: Mohan Prem Anand
authors.initials: MP
authors.lastname: Marimuthu
authors.affiliation: 1] Department of Plant Biology, University of California, Davis, California 95616, USA [2] Genome Center, University of California, Davis, California 95616, USA.
authors.forename: Ek Han
authors.initials: EH
authors.lastname: Tan
authors.affiliation: 1] Department of Plant Biology, University of California, Davis, California 95616, USA [2] Genome Center, University of California, Davis, California 95616, USA.
authors.forename: Shamoni
authors.initials: S
authors.lastname: Maheshwari
authors.affiliation: 1] Department of Plant Biology, University of California, Davis, California 95616, USA [2] Genome Center, University of California, Davis, California 95616, USA.
authors.forename: Isabelle M
authors.initials: IM
authors.lastname: Henry
authors.affiliation: Department of Plant Biology, University of California, Davis, California 95616, USA.
authors.forename: Brenda
authors.initials: B
authors.lastname: Marin-Rodriguez
authors.affiliation: Department of Plant Biology, University of California, Davis, California 95616, USA.
authors.forename: Guillaume
authors.initials: G
authors.lastname: Urtecho
authors.affiliation: Department of Plant Biology, University of California, Davis, California 95616, USA.
authors.forename: Jie
authors.initials: J
authors.lastname: Tan
authors.affiliation: Department of Plant Biology, University of California, Davis, California 95616, USA.
authors.forename: Kristina
authors.initials: K
authors.lastname: Thornhill
authors.affiliation: Department of Plant Biology, University of California, Davis, California 95616, USA.
authors.forename: Fan
authors.initials: F
authors.lastname: Zhu
authors.affiliation: Department of Plant Biology, University of California, Davis, California 95616, USA.
authors.forename: Aneesh
authors.initials: A
authors.lastname: Panoli
authors.affiliation: Department of Plant Biology, University of California, Davis, California 95616, USA.
authors.forename: Venkatesan
authors.initials: V
authors.lastname: Sundaresan
authors.affiliation: Department of Plant Biology, University of California, Davis, California 95616, USA.
authors.forename: Anne B
authors.initials: AB
authors.lastname: Britt
authors.affiliation: 1] Department of Plant Biology, University of California, Davis, California 95616, USA [2] Genome Center, University of California, Davis, California 95616, USA.
authors.forename: Luca
authors.initials: L
authors.lastname: Comai
authors.affiliation: 1] Department of Plant Biology, University of California, Davis, California 95616, USA [2] Howard-Hughes Medical Institute and the Gordon and Betty Moore Foundation, University of California, Davis, California 95616, USA.
authors.forename: Simon W L
authors.initials: SW
authors.lastname: Chan
mesh_terms: D017360:Arabidopsis / Q000235:genetics*; D005821:Genetic Techniques; D018745:Genome, Plant; D006238:Haploidy; D006720:Homozygote; D009154:Mutation; D010641:Phenotype