title: Characterization of bovine interferon α1: expression in yeast Pichia pastoris, biological activities, and physicochemical characteristics.
abstract: A bovine interferon α (BoIFNα) gene that included signal sequence was amplified from bovine liver genomic DNA. The gene was named BoIFN-α1 according to the position at which the encoded gene of the bovine IFN was located in the bovine genome. The sequence included a 23-amino-acid signal peptide and a 166-amino-acid mature peptide. The structural characteristics and phylogenetic relationships of the BoIFN-α1 gene were analyzed. A recombinant mature BoIFN-α1 (rBoIFN-α1) was expressed in the yeast Pichia pastoris. Physicochemical characteristics and antiviral activity were determined in vitro. Recombinant BoIFN-α1 was found to be highly sensitive to trypsin and stable at pH 2.0 or 65°C. It also exhibited antiviral activity, which was neutralized by a rabbit anti-rBoIFNα polyclonal antibody. This study revealed that rBoIFN-α1 has the typical characteristics of IFNα and can be used for both research and industrial application.
authors.affiliation: 1 College of Veterinary Medicine, Northeast Agricultural University , Harbin, China .
authors.forename: Jianwei
authors.initials: J
authors.lastname: Shao
authors.forename: Chong
authors.initials: C
authors.lastname: Cao
authors.forename: Jun
authors.initials: J
authors.lastname: Bao
authors.forename: Hongtao
authors.initials: H
authors.lastname: Liu
authors.forename: Tongquan
authors.initials: T
authors.lastname: Peng
authors.forename: Mingchun
authors.initials: M
authors.lastname: Gao
authors.forename: Junwei
authors.initials: J
authors.lastname: Wang
mesh_terms: D000818:Animals; D019138:Antibodies, Blocking / Q000378:metabolism; D000998:Antiviral Agents / Q000737:chemistry / Q000378:metabolism*; D002417:Cattle; D002460:Cell Line; D004847:Epithelial Cells / Q000187:drug effects* / Q000502:physiology; D015870:Gene Expression; D016678:Genome; D006801:Humans; D016898:Interferon-alpha / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D008099:Liver / Q000502:physiology*; D010802:Phylogeny; D010843:Pichia / Q000235:genetics; D011817:Rabbits
chemical_list: D019138:Antibodies, Blocking; D000998:Antiviral Agents; D016898:Interferon-alpha