title: Attenuation of veterinary antibiotics in full-scale vermicomposting of swine manure via the housefly larvae (Musca domestica).
abstract: Animal waste from concentrated swine farms is widely considered to be a source of environmental pollution, and the introduction of veterinary antibiotics in animal manure to ecosystems is rapidly becoming a major public health concern. A housefly larvae (Musca domestica) vermireactor has been increasingly adopted for swine manure value-added bioconversion and pollution control, but few studies have investigated its efficiency on antibiotic attenuation during manure vermicomposting. In this study we explored the capacity and related attenuation mechanisms of antibiotic degradation and its linkage with waste reduction by field sampling during a typical cycle (6 days) of full-scale larvae manure vermicomposting. Nine antibiotics were dramatically removed during the 6-day vermicomposting process, including tetracyclines, sulfonamides, and fluoroquinolones. Of these, oxytetracycline and ciprofloxacin exhibited the greater reduction rate of 23.8 and 32.9 mg m(-2), respectively. Environmental temperature, pH, and total phosphorus were negatively linked to the level of residual antibiotics, while organic matter, total Kjeldahl nitrogen, microbial respiration intensity, and moisture exhibited a positive effect. Pyrosequencing data revealed that the dominant phyla related to Firmicutes, Bacteroidetes, and Proteobacteria accelerated manure biodegradation likely through enzyme catalytic reactions, which may enhance antibiotic attenuation during vermicomposting.
authors.affiliation: 1] Institute of Environmental Sciences, College of Environmental and Resource Sciences, ZheJiang University, HangZhou, 310058, China [2] China Academy of West Region Development, ZheJiang University, HangZhou, 310058, China.
authors.forename: ZhiJian
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Agro-technology Extension Center at YuHang District, HangZhou, 311100, China.
authors.forename: JianGuo
authors.initials: J
authors.lastname: Shen
authors.affiliation: Institute of Environmental Sciences, College of Environmental and Resource Sciences, ZheJiang University, HangZhou, 310058, China.
authors.forename: Hang
authors.initials: H
authors.lastname: Wang
authors.affiliation: Institute of Environmental Sciences, College of Environmental and Resource Sciences, ZheJiang University, HangZhou, 310058, China.
authors.forename: Meng
authors.initials: M
authors.lastname: Liu
authors.affiliation: Institute of Soil Science, Chinese Academy of Sciences, East Beijing Road 71, NanJing, 210008, China.
authors.forename: LongHua
authors.initials: L
authors.lastname: Wu
authors.affiliation: Institute of Environmental Sciences, College of Environmental and Resource Sciences, ZheJiang University, HangZhou, 310058, China.
authors.forename: Fan
authors.initials: F
authors.lastname: Ping
authors.affiliation: Department of Civil and Environmental Engineering, University of Tennessee, Knoxville, TN, 37996-2010, USA.
authors.forename: Qiang
authors.initials: Q
authors.lastname: He
authors.affiliation: Institute of Environmental Sciences, College of Environmental and Resource Sciences, ZheJiang University, HangZhou, 310058, China.
authors.forename: HongYi
authors.initials: H
authors.lastname: Li
authors.affiliation: HangZhou TianYuan Agriculture Development Co., Ltd. HaiTuo Ave 55, XiaoShan District, HangZhou, 321103, China.
authors.forename: ChangFeng
authors.initials: C
authors.lastname: Zheng
authors.affiliation: Institute of Environmental Sciences, College of Environmental and Resource Sciences, ZheJiang University, HangZhou, 310058, China.
authors.forename: XinHua
authors.initials: X
authors.lastname: Xu
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents; D001673:Biodegradation, Environmental / Q000187:drug effects; D044822:Biodiversity; D006793:Houseflies; D008372:Manure / Q000382:microbiology / Q000469:parasitology*; D013552:Swine
chemical_list: D000900:Anti-Bacterial Agents; D008372:Manure