title: Anti-inflammatory and antioxidant activities of methanol extracts and alkaloid fractions of four Mexican medicinal plants of Solanaceae.
abstract: BACKGROUND
  Methanol extracts and alkaloid fractions of different parts of four plant species belonging to Solanaceae family and used in Mexican traditional medicine were investigated for their total phenolic contents, anti-inflammatory and antioxidant properties.
  
  
  METHODS
  The total phenolic compounds of each extract was determined according to the Folin-Ciocalteu method, while the in vitro radical scavenging activities of the extracts were assessed using the DPPH and ABTS radicals. The in vivo anti-inflammatory activity was determined using the TPA-induced mouse ear edema model.
  
  
  RESULTS
  The methanol extracts contained the highest concentrations of phenolic compounds and also exhibited the best reducing power on the DPPH and ABTS radicals, in a concentration-dependent fashion. However, the anti-inflammatory activity did not follow the same trend, as some alkaloid fractions that showed low radical reducing power exhibited the strongest anti-inflammatory activity.
  
  
  CONCLUSIONS
  The methanol extract obtained from the flowers of Nicotiana glauca presented the best overall performance with the largest amount of phenolic compounds (111 µg garlic acid equivalents/g of extract), the best antioxidant activity (94.80% inhibition of DPPH and 97.57% of ABTS) and the highest anti-inflammatory activity (81.93% inhibition of the inflammation).
authors.affiliation: Facultad de Química, Universidad Autónoma de Querétaro, Cerro de las Campanas s/n, C.P. 76010 Querétaro, Querétaro, México.
authors.forename: Dora M
authors.initials: DM
authors.lastname: Gutiérrez A
authors.affiliation: Facultad de Química, Universidad Autónoma de Querétaro, Cerro de las Campanas s/n, C.P. 76010 Querétaro, Querétaro, México.
authors.forename: Moustapha
authors.initials: M
authors.lastname: Bah
authors.affiliation: Centro de Investigaciones Químicas, Lab. 9, Química de Productos Naturales y Evaluaciones Farmacológicas. Universidad Autónoma del Estado de Morelos, Av. Universidad 1001, C.P. 62209. Col. Chamilpa. Cuernavaca, Morelos México.
authors.forename: María L
authors.initials: ML
authors.lastname: Garduño R
authors.affiliation: Facultad de Química, Universidad Autónoma de Querétaro, Cerro de las Campanas s/n, C.P. 76010 Querétaro, Querétaro, México.
authors.forename: Sandra O
authors.initials: SO
authors.lastname: Mendoza D
authors.affiliation: Facultad de Ciencias Naturales, Herbario de Querétaro "Dr. Jerzy Rzedowski" (QMEX), Universidad Autónoma de Querétaro, avenida de las Ciencias s/n, C.P. 76230, Juriquilla, Querétaro, México.
authors.forename: Valentina
authors.initials: V
authors.lastname: Serrano C
mesh_terms: D000470:Alkaloids / Q000008:administration & dosage* / Q000032:analysis / Q000302:isolation & purification; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage* / Q000032:analysis / Q000302:isolation & purification; D000975:Antioxidants / Q000008:administration & dosage* / Q000032:analysis / Q000302:isolation & purification; D004487:Edema / Q000188:drug therapy*; D006801:Humans; D008800:Mexico; D051379:Mice; D010936:Plant Extracts / Q000008:administration & dosage* / Q000032:analysis / Q000302:isolation & purification; D018515:Plant Leaves / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry / Q000145:classification; D019657:Solanaceae / Q000737:chemistry* / Q000145:classification
chemical_list: D000470:Alkaloids; D000893:Anti-Inflammatory Agents; D000975:Antioxidants; D010936:Plant Extracts
keywords: Solanaceae family; anti-inflammatory activity; antioxidant activity