title: Pre-clinical evaluation of the osteogenic potential of bone morphogenetic protein-2 loaded onto a particulate porcine bone biomaterial.
abstract: OBJECTIVE
  The objective of study was to determine the osteogenic potential of bone morphogenetic protein-2 (BMP-2) loaded onto a particulate porcine bone mineral (PBM) biomaterial using a sinus augmentation model.
  
  
  METHODS
  Release kinetics of BMP-2/PBM was determined in vitro. Eight rabbits received BMP-2/PBM or PBM alone into contra-lateral sinus sites. The animals were killed following a 2-week healing interval for micro-CT and histometrical analysis.
  
  
  RESULTS
  Approximately 40% of the BMP-2 was released from PBM over the first 3 days in vitro; release maintained at a reduced level through day 21. In vivo, total augmented implant volume did not differ significantly between treatments. However, local bone formation was enhanced in the BMP-2/PBM group compared with PBM control (10.5% versus 6.6%; p = 0.03), specifically in the central aspect of the PBM implant (14.2% versus 5.5%; p < 0.01) and adjoining the Schneiderian membrane (11.9% versus 5.0%; p < 0.05). There were no significant overall differences in residual biomaterial and fibrovascular tissue.
  
  
  CONCLUSIONS
  Bone morphogenetic protein-2 enhanced local bone formation in the rabbit maxillary sinus model following implantation using a PBM carrier.
authors.affiliation: Department of Periodontology, Research Institute for Periodontal Regeneration, College of Dentistry, Yonsei University, Seoul, Korea.
authors.forename: Jeyoung
authors.initials: J
authors.lastname: Yon
authors.forename: Jung-Seok
authors.initials: JS
authors.lastname: Lee
authors.forename: Hyun-Chang
authors.initials: HC
authors.lastname: Lim
authors.forename: Min-Soo
authors.initials: MS
authors.lastname: Kim
authors.forename: Ji-Youn
authors.initials: JY
authors.lastname: Hong
authors.forename: Seong-Ho
authors.initials: SH
authors.lastname: Choi
authors.forename: Ui-Won
authors.initials: UW
authors.lastname: Jung
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000627:therapeutic use*; D055396:Bone Morphogenetic Protein 2 / Q000493:pharmacokinetics / Q000627:therapeutic use*; D018786:Bone Substitutes / Q000627:therapeutic use*; D004353:Drug Evaluation, Preclinical; D015726:Giant Cells / Q000473:pathology; D007091:Image Processing, Computer-Assisted / Q000379:methods; D021621:Imaging, Three-Dimensional / Q000379:methods; D008297:Male; D008443:Maxillary Sinus / Q000187:drug effects / Q000473:pathology; D009297:Nasal Mucosa / Q000187:drug effects / Q000473:pathology; D010012:Osteogenesis / Q000187:drug effects*; D011817:Rabbits; D011897:Random Allocation; D059546:Sinus Floor Augmentation / Q000379:methods*; D013552:Swine; D055114:X-Ray Microtomography / Q000379:methods
chemical_list: D001672:Biocompatible Materials; D055396:Bone Morphogenetic Protein 2; D018786:Bone Substitutes
keywords: Schneiderian membrane; animal model; bone morphogenetic protein-2; bone regeneration; xenograft