title: Proteomic approaches in research of cyanobacterial photosynthesis.
abstract: Oxygenic photosynthesis in cyanobacteria, algae, and plants is carried out by a fabulous pigment-protein machinery that is amazingly complicated in structure and function. Many different approaches have been undertaken to characterize the most important aspects of photosynthesis, and proteomics has become the essential component in this research. Here we describe various methods which have been used in proteomic research of cyanobacteria, and demonstrate how proteomics is implemented into on-going studies of photosynthesis in cyanobacterial cells.
authors.affiliation: Laboratory of Molecular Plant Biology, Department of Biochemistry, University of Turku, 20014, Turku, Finland. natbat@utu.fi.
authors.forename: Natalia
authors.initials: N
authors.lastname: Battchikova
authors.affiliation: Laboratory of Molecular Plant Biology, Department of Biochemistry, University of Turku, 20014, Turku, Finland.
authors.forename: Martina
authors.initials: M
authors.lastname: Angeleri
authors.affiliation: Laboratory of Molecular Plant Biology, Department of Biochemistry, University of Turku, 20014, Turku, Finland.
authors.forename: Eva-Mari
authors.initials: EM
authors.lastname: Aro
mesh_terms: D001426:Bacterial Proteins / Q000032:analysis; D000458:Cyanobacteria / Q000166:cytology / Q000502:physiology*; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D007553:Isotope Labeling / Q000379:methods; D013058:Mass Spectrometry / Q000379:methods; D008565:Membrane Proteins / Q000032:analysis; D010788:Photosynthesis; D011499:Protein Processing, Post-Translational; D040901:Proteomics / Q000379:methods*; D060431:Thylakoid Membrane Proteins / Q000032:analysis / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D008565:Membrane Proteins; D060431:Thylakoid Membrane Proteins
keywords: Cyanobacteria; Mass spectrometry; Photosynthesis; Post-translational modification; Protein identification; Protein quantitation; Proteomics