title: Effects of targeted modulation of miR-762 on expression of the IFITM5 gene in Saos-2 cells.
abstract: Interferon-induced transmembrane protein 5 (IFITM5) is an osteoblast-specific membrane protein that plays an important role in the mineralization of the matrix in mature osteoblasts. However, understanding of the regulatory mechanism of IFITM5 expression is limited. Emerging evidence indicates that microRNAs (miRNAs) act as pivotal regulators in various biological processes including osteoblast proliferation and differentiation. This study aimed to investigate the impact of miRNAs on IFITM5 expression. Bioinformatic analyses predicted that miR-762 would be a potential regulator of IFITM5. A Dual-Luciferase Reporter Assay System indicated that miR-762 could bond with the 3'untranslated region (3'UTR) of IFITM5 via wild-type or mutant recombinant vectors and Western blotting verified that miR-762 negatively regulated IFITM5 expression. Collectively, these data indicate that miR-762 is a novel regulator of IFITM5 and that it suppresses the expression of IFITM5 in Saos-2 cells.
authors.affiliation: Key Laboratory for Rare Disease Research of Shandong Province, Key Laboratory for Biotech Drugs of the Ministry of Health, Shandong Medical Biotechnological Center, Shandong Academy of Medical Sciences, Ji'nan, Shandong, China; ; School of Medicine and Life Sciences, University of Jinan-Shandong Academy of Medical Science, Ji'nan, Shandong, China.
authors.forename: Xinkai
authors.initials: X
authors.lastname: Mo
authors.affiliation: Key Laboratory for Rare Disease Research of Shandong Province, Key Laboratory for Biotech Drugs of the Ministry of Health, Shandong Medical Biotechnological Center, Shandong Academy of Medical Sciences, Ji'nan, Shandong, China;
authors.forename: Yanqin
authors.initials: Y
authors.lastname: Lu
authors.affiliation: Key Laboratory for Rare Disease Research of Shandong Province, Key Laboratory for Biotech Drugs of the Ministry of Health, Shandong Medical Biotechnological Center, Shandong Academy of Medical Sciences, Ji'nan, Shandong, China;
authors.forename: Jinxiang
authors.initials: J
authors.lastname: Han
keywords: IFITM5 gene; miR-762; mineralization; target gene