title: Structure modeling and dynamics driven mutation and phosphorylation analysis of Beta-amyloid peptides.
abstract: The most common characteristics of diverse age-related neurodegenerative diseases are aggregation and accumulation of the misfolded protein in the brain. Alzheimer׳s disease (AD) is one of these protein conformational diseases. Extracellular accumulation of amyloid β (Aβ) is one the neuropathological hallmarks of Alzheimer disease. Various studies have shown that mutation in specific hydrophobic region of Aβ protein inhibit the formation of β sheet, thus aggregation of this protein is stalled. The identification of such mutation in Aβ protein can help us in elucidating the etiology of sporadic Aβ. In our study we have selected three positions: 19ILU, 21ALA and 41ILU in Aβ protein based on their hydrophobic nature and substituted them with PRO ( βSheet breaker). The effects of the substitutions were analysed using molecular dynamics simulation studies. The results validated that the mutations in the specified regions change the hydrophobicity of the protein and the βsheet formation was declined to zero per cent.
authors.affiliation: Department of Biotechnology and Bioinformatics, padmashree Dr.D.Y.Patil University, Belapur-400614, Navi Mumbai, India.
authors.forename: Sunil Kumar
authors.initials: SK
authors.lastname: Singh
authors.affiliation: Department of Biotechnology and Bioinformatics, padmashree Dr.D.Y.Patil University, Belapur-400614, Navi Mumbai, India.
authors.forename: Ankita
authors.initials: A
authors.lastname: Singh
authors.affiliation: Department of Biotechnology and Bioinformatics, padmashree Dr.D.Y.Patil University, Belapur-400614, Navi Mumbai, India.
authors.forename: Ved
authors.initials: V
authors.lastname: Prakash
authors.affiliation: Department of Biotechnology and Bioinformatics, padmashree Dr.D.Y.Patil University, Belapur-400614, Navi Mumbai, India.
authors.forename: Selvaa Kumar
authors.initials: SK
authors.lastname: C
keywords: Alzheimer׳s disease; Amyloid-beta peptide; Amyloid-beta precursor protein; Argus Lab; Gromos; PPI-Pred; UCSF Chimera