title: Cytoprotective alginate/polydopamine core/shell microcapsules in microbial encapsulation.
abstract: Chemical encapsulation of microbes in threedimensional polymeric microcapsules promises various applications, such as cell therapy and biosensors, and provides a basic platform for studying microbial communications. However, the cytoprotection of microbes in the microcapsules against external aggressors has been a major challenge in the field of microbial microencapsulation, because ionotropic hydrogels widely used for microencapsulation swell uncontrollably, and are physicochemically labile. Herein, we developed a simple polydopamine coating for obtaining cytoprotective capability of the alginate capsule that encapsulated Saccharomyces cerevisiae. The resulting alginate/ polydopamine core/shell capsule was mechanically tough, prevented gel swelling and cell leakage, and increased resistance against enzymatic attack and UV-C irradiation. We believe that this multifunctional core/shell structure will provide a practical tool for manipulating microorganisms inside the microcapsules.
authors.affiliation: Center for Cell-Encapsulation Research, Department of Chemistry, KAIST, Daejeon 305-701 (Korea).
authors.forename: Beom Jin
authors.initials: BJ
authors.lastname: Kim
authors.forename: Taegyun
authors.initials: T
authors.lastname: Park
authors.forename: Hee Chul
authors.initials: HC
authors.lastname: Moon
authors.forename: So-Young
authors.initials: SY
authors.lastname: Park
authors.forename: Daewha
authors.initials: D
authors.lastname: Hong
authors.forename: Eun Hyea
authors.initials: EH
authors.lastname: Ko
authors.forename: Ji Yup
authors.initials: JY
authors.lastname: Kim
authors.forename: Jong Wook
authors.initials: JW
authors.lastname: Hong
authors.forename: Sang Woo
authors.initials: SW
authors.lastname: Han
authors.forename: Yang-Gyun
authors.initials: YG
authors.lastname: Kim
authors.forename: Insung S
authors.initials: IS
authors.lastname: Choi
mesh_terms: D000464:Alginates / Q000737:chemistry*; D002214:Capsules / Q000737:chemistry*; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D007211:Indoles / Q000737:chemistry*; D018613:Microscopy, Confocal; D008858:Microscopy, Phase-Contrast; D011108:Polymers / Q000737:chemistry*; D020011:Protective Agents / Q000737:chemistry*; D012441:Saccharomyces cerevisiae / Q000737:chemistry / Q000254:growth & development / Q000528:radiation effects; D014466:Ultraviolet Rays
chemical_list: D000464:Alginates; D002214:Capsules; D006603:Hexuronic Acids; D007211:Indoles; D011108:Polymers; D020011:Protective Agents; C568283:polydopamine; D020723:Glucuronic Acid
keywords: cytoprotection; encapsulation; gels; nanostructures; polydopamine