title: [Preliminary study of Dioscorea bulbifera plantlet microtuber in vitro induction].
abstract: OBJECTIVE
  In order to provide a theoretical basis for the microtuber factory production and its germplasm resources preservation, in vitro induction of Dioscorea bulbifera plantlet microtubers was studied.
  
  
  METHODS
  Through plant tissue culture technique and single factor experiment method, stems with buds of Dioscorea bulbifera plantlets as explants, the effects of various factors such as sugar, inorganic salt, cultivation mode, activated carbon and physiological state of stems with buds on in vitro induction of Dioscorea bulbifera microtubers were studied.
  
  
  RESULTS
  The optimal sugar of Dioscorea bulbifera microtuber in vitro induction was 60 g/L sucrose or 90 g/L white sugar. The best inorganic salt concentration of Dioscorea bulbifera microtuber in vitro induction was MS. The best culture method of Dioscorea bulbifera microtuber in vitro induction was solid-liquid double layer culture. Activated carbon had a significant effect on Dioscorea bulbifera microtuber in vitro induction, whose optimum concentration was 0.03%. More mature the stem with buds was, the shorter the time of microtuber formation need.
  
  
  CONCLUSIONS
  This experiment establishes a rapid method of Dioscorea bulbifera microtuber in vitro induction for the first time,which provides a new way for the application of Dioscorea bulbifera microtubers in agricultural production.
authors.forename: Sen-Rong
authors.initials: SR
authors.lastname: Hong
authors.forename: Ming-Hua
authors.initials: MH
authors.lastname: Yin
mesh_terms: D003470:Culture Media; D027742:Dioscorea / Q000187:drug effects / Q000254:growth & development*; D010937:Plant Growth Regulators / Q000494:pharmacology; D018547:Plant Stems / Q000187:drug effects / Q000254:growth & development*; D035281:Plant Tubers / Q000187:drug effects / Q000254:growth & development*; D013395:Sucrose / Q000494:pharmacology; D046509:Tissue Culture Techniques / Q000379:methods*
chemical_list: D003470:Culture Media; D010937:Plant Growth Regulators; D013395:Sucrose