title: Specific recognition of guanines in non-duplex regions of nucleic acids with potassium tungstate and hydrogen peroxide.
abstract: Structural features of nucleic acids have become an integral part of current biomedical research. Highly selective and readily performed methods with little toxicity that target guanosines in non-duplex nucleic acids are needed, which led us to search for an effective agent for guanosine sequencing. Treatment of DNA or RNA with potassium tungstate and hydrogen peroxide produced damaged guanosines in DNA or RNA sequences. The damaged guanosines in non-duplex DNA could be cleaved by hot piperidine. Similarly, damaged guanosines in non-duplex RNA could be cleaved by aniline acetate. We could identify structural features of nucleic acid using this strategy instead of dimethyl sulphate and Ribonuclease T1.
authors.affiliation: College of Chemistry and Molecular Sciences, Key Laboratory of Biomedical Polymers of Ministry of Education, State Key Laboratory of Virology, Wuhan University, Hubei, Wuhan 430072, P. R. China.
authors.forename: Wuxiang
authors.initials: W
authors.lastname: Mao
authors.affiliation: College of Chemistry and Molecular Sciences, Key Laboratory of Biomedical Polymers of Ministry of Education, State Key Laboratory of Virology, Wuhan University, Hubei, Wuhan 430072, P. R. China.
authors.forename: Xiaowei
authors.initials: X
authors.lastname: Xu
authors.affiliation: College of Chemistry and Molecular Sciences, Key Laboratory of Biomedical Polymers of Ministry of Education, State Key Laboratory of Virology, Wuhan University, Hubei, Wuhan 430072, P. R. China.
authors.forename: Huan
authors.initials: H
authors.lastname: He
authors.affiliation: College of Chemistry and Molecular Sciences, Key Laboratory of Biomedical Polymers of Ministry of Education, State Key Laboratory of Virology, Wuhan University, Hubei, Wuhan 430072, P. R. China.
authors.forename: Rong
authors.initials: R
authors.lastname: Huang
authors.affiliation: College of Chemistry and Molecular Sciences, Key Laboratory of Biomedical Polymers of Ministry of Education, State Key Laboratory of Virology, Wuhan University, Hubei, Wuhan 430072, P. R. China.
authors.forename: Xi
authors.initials: X
authors.lastname: Chen
authors.affiliation: College of Chemistry and Molecular Sciences, Key Laboratory of Biomedical Polymers of Ministry of Education, State Key Laboratory of Virology, Wuhan University, Hubei, Wuhan 430072, P. R. China.
authors.forename: Heng
authors.initials: H
authors.lastname: Xiao
authors.affiliation: College of Chemistry and Molecular Sciences, Key Laboratory of Biomedical Polymers of Ministry of Education, State Key Laboratory of Virology, Wuhan University, Hubei, Wuhan 430072, P. R. China.
authors.forename: Zhenduo
authors.initials: Z
authors.lastname: Yu
authors.affiliation: College of Chemistry and Molecular Sciences, Key Laboratory of Biomedical Polymers of Ministry of Education, State Key Laboratory of Virology, Wuhan University, Hubei, Wuhan 430072, P. R. China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Liu
authors.affiliation: College of Chemistry and Molecular Sciences, Key Laboratory of Biomedical Polymers of Ministry of Education, State Key Laboratory of Virology, Wuhan University, Hubei, Wuhan 430072, P. R. China xzhou@whu.edu.cn.
authors.forename: Xiang
authors.initials: X
authors.lastname: Zhou
mesh_terms: D004247:DNA / Q000737:chemistry*; D004277:DNA, Single-Stranded / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D006147:Guanine / Q000737:chemistry*; D006861:Hydrogen Peroxide / Q000737:chemistry*; D008958:Models, Molecular; D009690:Nucleic Acid Conformation; D010084:Oxidation-Reduction; D012313:RNA / Q000737:chemistry*; D017973:Tungsten Compounds / Q000737:chemistry*
chemical_list: D004277:DNA, Single-Stranded; D017973:Tungsten Compounds; D006147:Guanine; D012313:RNA; D004247:DNA; D006861:Hydrogen Peroxide; C045951:tungstate