title: Detection of protein cage assembly with bisarsenic fluorescent probes.
abstract: We describe a method for the detection of specific protein-protein interactions in protein cages through the exploitation of designed binding sites for bisarsenic fluorescent probes. These sites are engineered to be protein-protein interface specific. We have adapted this method to ferritins; however, it could conceivably be applied to other protein cages. It is thought that this technique could be utilized in the thermodynamic and kinetic characterization of cage assembly mechanisms and in the high-throughput screening of protein cage libraries for the discovery of proteins with new assembly properties or of optimized conditions for assembly.
authors.affiliation: Department of Chemistry, King's College London, Guys Campus, London, SE1 1DB, UK, Thomas.cornell@kcl.ac.uk.
authors.forename: Thomas A
authors.initials: TA
authors.lastname: Cornell
authors.forename: Brendan P
authors.initials: BP
authors.lastname: Orner
mesh_terms: D001151:Arsenic / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D011485:Protein Binding; D011489:Protein Denaturation; D015202:Protein Engineering / Q000379:methods*; D055503:Protein Multimerization; D011506:Proteins / Q000737:chemistry*; D013050:Spectrometry, Fluorescence
chemical_list: D005456:Fluorescent Dyes; D011506:Proteins; D001151:Arsenic