title: Model-potential-free analysis of small angle scattering of proteins in solution: insights into solvent effects on protein-protein interaction.
abstract: To extract protein-protein interaction from experimental small-angle scattering of proteins in solutions using liquid state theory, a model potential consisting of a hard-sphere repulsive potential and the excess interaction potential has been introduced. In the present study, we propose a model-potential-free integral equation method that extracts the excess interaction potential by using the experimental small-angle scattering data without specific model potential such as the Derjaguin-Landau-Verwey-Overbeek (DLVO)-type model. Our analysis of experimental small-angle X-ray scattering data for lysozyme solution shows both the stabilization of contact configurations of protein molecules and a large activation barrier against the formation of the contact configurations in addition to the screened Coulomb repulsion. These characteristic features, which are not well-described by the DLVO-type model, are interpreted as solvent effects.
authors.affiliation: Department of Chemistry, Faculty of Science, Okayama University, 3-1-1 Tsushima-Naka, Kita-ku, Okayama 700-8530, Japan. sumi@okayama-u.ac.jp.
authors.forename: Tomonari
authors.initials: T
authors.lastname: Sumi
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Imamura
authors.forename: Takeshi
authors.initials: T
authors.lastname: Morita
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Isogai
authors.forename: Keiko
authors.initials: K
authors.lastname: Nishikawa
mesh_terms: D008958:Models, Molecular; D009113:Muramidase / Q000737:chemistry* / Q000378:metabolism*; D011485:Protein Binding / Q000187:drug effects; D053838:Scattering, Small Angle; D012996:Solutions; D012997:Solvents / Q000494:pharmacology*; D014961:X-Ray Diffraction
chemical_list: D012996:Solutions; D012997:Solvents; D009113:Muramidase