title: HPLC method for identification and quantification of three active substances in a dermatological preparation--Viosept ointment.
abstract: The study was aimed at developing a HPLC method to identify and quantify domiphen bromide, tripelennamine hydrochloride and clioquinol in Viosept ointment. The tested substances were successfully separated using Inertsil ODS-3 (250 x 4.6 mm, 5 μm) as a stationary phase and a gradient elution. Detection at 310 nm wavelength was applied for tripelennamine hydrochloride and clioquinol, and at 215 nm wavelength for domiphen bromide. Methods of extraction of the tested substances were developed: domiphen bromide and clioquinol were extracted with acetone from heated solutions, and tripelennamine hydrochloride was extracted in a hexane-water system. Validation procedure confirmed the method to be sufficiently selective, precise and accurate. Correlation coefficients of calibration curves pointed out that they were linear within the examined concentration range.
authors.forename: Marena
authors.initials: M
authors.lastname: Podolska
authors.forename: Anna
authors.initials: A
authors.lastname: Kulik
authors.forename: Wanda
authors.initials: W
authors.lastname: Białecka
authors.forename: Barbara
authors.initials: B
authors.lastname: Kwiatkowska-Puchniarz
authors.forename: Aleksander
authors.initials: A
authors.lastname: Mazurek
mesh_terms: D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000592:standards*; D007464:Clioquinol / Q000032:analysis*; D003879:Dermatologic Agents / Q000032:analysis*; D004338:Drug Combinations; D016014:Linear Models; D009824:Ointments; D000644:Quaternary Ammonium Compounds / Q000032:analysis*; D012015:Reference Standards; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet; D014309:Tripelennamine / Q000032:analysis*
chemical_list: D003879:Dermatologic Agents; D004338:Drug Combinations; D009824:Ointments; D000644:Quaternary Ammonium Compounds; C016185:clioquinol, domiphen bromide, tripelennamine hydrochloride drug combination; D014309:Tripelennamine; C007556:domiphen; D007464:Clioquinol