title: [The biological evaluation of depression severity: a novel method for the determination of platelet serotonin concentration].
abstract: OBJECTIVE
  To develop a highly sensitive method for the determination of platelet-rich plasma serotonin concentration for every-day clinical practice.
  
  
  METHODS
  The method was developed for determination of serotonin in plateled-rich plasma. Serotonin was determined using high performance liquid chromatography with fluorescent detection and hematologic analyzer.
  
  
  CONCLUSIONS
  In healthy people, concentrations of platelet serotonin were similar to those reported in literature: from 2.19 to 4.87 nmol/109 platelets. In patients with depression (n=30), platelet serotonin concentrations were lower (from 0.11 to 2.89 nmol/109 platelets) and correlated with the severity of depression. The method may be used in clinical purposes.
authors.forename: M V
authors.initials: MV
authors.lastname: Bezrukov
authors.forename: Iu E
authors.initials: IuE
authors.lastname: Shilov
authors.forename: N V
authors.initials: NV
authors.lastname: Shestakova
authors.forename: T P
authors.initials: TP
authors.lastname: Kliushnik
mesh_terms: D001792:Blood Platelets / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003866:Depressive Disorder / Q000097:blood*; D006801:Humans; D010976:Platelet Count; D012701:Serotonin / Q000097:blood*; D012720:Severity of Illness Index
chemical_list: D012701:Serotonin