title: Quantitative proteomics reveals the dynamics of protein changes during Drosophila oocyte maturation and the oocyte-to-embryo transition.
abstract: The onset of development is marked by two major, posttranscriptionally controlled, events: oocyte maturation (release of the prophase I primary arrest) and egg activation (release from the secondary meiotic arrest). Using quantitative mass spectrometry, we previously described proteome remodeling during Drosophila egg activation. Here, we describe our quantitative mass spectrometry-based analysis of the changes in protein levels during Drosophila oocyte maturation. This study presents the first quantitative survey, to our knowledge, of proteome changes accompanying oocyte maturation in any organism and provides a powerful resource for identifying both key regulators and biological processes driving this critical developmental window. We show that Muskelin, found to be up-regulated during oocyte maturation, is required for timely nurse cell nuclei clearing from mature egg chambers. Other proteins up-regulated at maturation are factors needed not only for late oogenesis but also completion of meiosis and early embryogenesis. Interestingly, the down-regulated proteins are predominantly involved in RNA processing, translation, and RNAi. Integrating datasets on the proteome changes at oocyte maturation and egg activation uncovers dynamics in proteome remodeling during the change from oocyte to embryo. Notably, 66 proteins likely act uniquely during late oogenesis, because they are up-regulated at maturation and down-regulated at activation. We find down-regulation of this class of proteins to be mediated partially by APC/C(CORT), a meiosis-specific form of the E3 ligase anaphase promoting complex/cyclosome (APC/C).
authors.affiliation: Whitehead Institute, Cambridge, MA 02142;
authors.forename: Iva
authors.initials: I
authors.lastname: Kronja
authors.affiliation: Whitehead Institute, Cambridge, MA 02142; Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02138; and.
authors.forename: Zachary J
authors.initials: ZJ
authors.lastname: Whitfield
authors.affiliation: Whitehead Institute, Cambridge, MA 02142;
authors.forename: Bingbing
authors.initials: B
authors.lastname: Yuan
authors.affiliation: European Molecular Biology Laboratory, Proteomics Core Facility, 69117 Heidelberg, Germany.
authors.forename: Kristina
authors.initials: K
authors.lastname: Dzeyk
authors.affiliation: European Molecular Biology Laboratory, Proteomics Core Facility, 69117 Heidelberg, Germany.
authors.forename: Joanna
authors.initials: J
authors.lastname: Kirkpatrick
authors.affiliation: European Molecular Biology Laboratory, Proteomics Core Facility, 69117 Heidelberg, Germany.
authors.forename: Jeroen
authors.initials: J
authors.lastname: Krijgsveld
authors.affiliation: Whitehead Institute, Cambridge, MA 02142; Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02138; and weaver@wi.mit.edu.
authors.forename: Terry L
authors.initials: TL
authors.lastname: Orr-Weaver
mesh_terms: D064173:Anaphase-Promoting Complex-Cyclosome / Q000378:metabolism; D000818:Animals; D029721:Drosophila Proteins / Q000378:metabolism*; D004331:Drosophila melanogaster; D004625:Embryo, Nonmammalian / Q000166:cytology / Q000378:metabolism*; D009865:Oocytes / Q000166:cytology / Q000378:metabolism*; D014176:Protein Biosynthesis / Q000502:physiology; D020543:Proteome / Q000378:metabolism*; D040901:Proteomics / Q000379:methods; D012323:RNA Processing, Post-Transcriptional / Q000502:physiology
chemical_list: D029721:Drosophila Proteins; D020543:Proteome; D064173:Anaphase-Promoting Complex-Cyclosome
keywords: Muskelin; embryo; meiosis; oocyte; spindle