title: Selective extraction based on poly(MAA-VB-EGMDA) monolith followed by HPLC for determination of hordenine in plasma and urine samples.
abstract: Hordenine is an active compound found in several foods, herbs and beer. In this work, a novel sorbent was fabricated for selective solid-phase extraction (SPE) of hordenine in biological samples. The organic polymer sorbent was synthesized in one step in the plastic barrel of a syringe by a pre-polymerization solution consisting of methacrylic acid (MAA), 4-vinylphenylboronic acid (VB) and ethylene glycol dimethacrylate (EGDMA). The conditions for preparation were optimized to generate a poly(MAA-VB-EGMDA) monolith with good permeability. The monolith exhibited good enrichment efficiency towards hordenine. By using tyramine as the internal standard, a poly(MAA-VB-EGMDA)-based SPE-HPLC method was established for analysis of hordenine. Conditions for SPE, including volume of eluting solvent, pH of sample solution, sampling rate and sample volume, were optimized. The proposed SPE-HPLC method presented good linearity (R(2)  = 0.9992) within 10-2000 ng/mL and the detection limits was 3 ng/mL, which is significantly more sensitive than reported methods. The method was also applied in plasma and urine samples; good capability of removing matrices was observed, while hordenine in low content was well extracted and enriched. The recoveries were from 90.6 to 94.7% and from 89.3 to 91.5% for the spiked plasma and urine samples, respectively, with the relative standard deviations <4.7%.
authors.affiliation: Department of Pharmacy, The Third Hospital of Wuhan, Wuhan, Hubei, 430060, People's Republic of China.
authors.forename: Yonggang
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Department of Pharmacy, The Third Hospital of Wuhan, Wuhan, Hubei, 430060, People's Republic of China.
authors.forename: Junhua
authors.initials: J
authors.lastname: Meng
authors.affiliation: Department of Pharmacy, The Third Hospital of Wuhan, Wuhan, Hubei, 430060, People's Republic of China.
authors.forename: Jili
authors.initials: J
authors.lastname: Zou
authors.affiliation: Department of Pharmacy, The Third Hospital of Wuhan, Wuhan, Hubei, 430060, People's Republic of China.
authors.forename: Jing
authors.initials: J
authors.lastname: An
mesh_terms: D001897:Boronic Acids / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005026:Ethylene Glycols / Q000737:chemistry*; D006801:Humans; D016014:Linear Models; D008689:Methacrylates / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D052616:Solid Phase Extraction / Q000295:instrumentation / Q000379:methods*; D014439:Tyramine / Q000031:analogs & derivatives* / Q000097:blood / Q000737:chemistry / Q000652:urine; D014753:Vinyl Compounds / Q000737:chemistry*
chemical_list: C427523:4-vinylphenyl boronic acid; D001897:Boronic Acids; D005026:Ethylene Glycols; D008689:Methacrylates; D014753:Vinyl Compounds; C502776:poly(methacrylic acid-ethylene glycol dimethacrylate); C007964:hordenine; D014439:Tyramine
keywords: hordenine; plasma; poly(MAA-VB-EGMDA); selective extraction; urine