title: A novel rose hip preparation with enhanced anti-inflammatory and chondroprotective effects.
abstract: Rose hip powder (RHP) alleviates osteoarthritis (OA) due to its anti-inflammatory and cartilage-protective properties. Substances contained in RHP might contribute to its clinical efficacy. The activity of two RHP (i.e., RH-A, from the whole fruit, RH-B, from fruits without seeds) was investigated in human peripheral blood leukocytes (PBL) and primary chondrocytes (NHAC-kn). RH-A and RH-B diminished the secretion of chemokines and cytokines in LPS/IFN-γ-activated PBL, including CCL5/RANTES, CXCL10/IP-10, interleukin- (IL-) 6, and IL-12. Most effects were transcriptional, since gene expression levels were significantly influenced by RH-A and RH-B. In IL-1β treated normal chondrocytes (NHAC-kn), both RH preparations reduced the expression of matrix metalloproteinase- (MMP-) 1, MMP-3, and MMP-13 and ADAMTS-4. These changes are associated with diminished inflammatory damage or cartilage erosion. Principal component analysis revealed that (1) RH-A and RH-B modified a large pattern of biomarkers, and (2) RH-B outperformed RH-A. Furthermore, RH-B contained more chondroprotective and anti-inflammatory constituents than RH-A. Thus, RHP contributed to restore cellular homeostasis in PBL and chondrocytes. RH preparations from fruits without seeds are thus expected to have an improved OA-preventive or OA-therapeutic profile, as subsequently shown in a related clinical trial.
authors.affiliation: DSM Nutritional Products Ltd., Department of Human Nutrition & Health, P.O. Box 2676, 4002 Basel, Switzerland.
authors.forename: Joseph
authors.identifier: 0000-0002-6174-6063
authors.initials: J
authors.lastname: Schwager
authors.affiliation: DSM Nutritional Products Ltd., Department of Human Nutrition & Health, P.O. Box 2676, 4002 Basel, Switzerland.
authors.forename: Nathalie
authors.identifier: 0000-0002-7091-6896
authors.initials: N
authors.lastname: Richard
authors.affiliation: DSM Nutritional Products Ltd., Department of Human Nutrition & Health, P.O. Box 2676, 4002 Basel, Switzerland.
authors.forename: Rotraut
authors.initials: R
authors.lastname: Schoop
authors.affiliation: DSM Nutritional Products Ltd., Department of Human Nutrition & Health, P.O. Box 2676, 4002 Basel, Switzerland.
authors.forename: Swen
authors.initials: S
authors.lastname: Wolfram
mesh_terms: D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D002478:Cells, Cultured; D019902:Chondrocytes / Q000187:drug effects / Q000378:metabolism; D016207:Cytokines / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D007962:Leukocytes / Q000187:drug effects / Q000378:metabolism; D010003:Osteoarthritis / Q000188:drug therapy* / Q000235:genetics / Q000378:metabolism; D008517:Phytotherapy; D028321:Plant Preparations / Q000494:pharmacology; D027826:Rosa
chemical_list: D000893:Anti-Inflammatory Agents; D016207:Cytokines; D028321:Plant Preparations