title: siRNA targeting stathmin inhibits invasion and enhances chemotherapy sensitivity of stem cells derived from glioma cell lines.
abstract: Glioma is one of the most highly angiogenic tumors, and glioma stem cells (GSCs) are responsible for resistance to chemotherapy and radiotherapy, as well as recurrence after operation. Stathmin is substantial for mitosis and plays an important role in proliferation and migration of glioma-derived endothelial cells. However, the relationship between stathmin and GSCs is incompletely understood. Here we isolated GSCs from glioma cell lines U87MG and U251, and then used siRNA targeting stathmin for silencing. We showed that silencing of stathmin suppressed the proliferation, increased the apoptosis rate, and arrested the cell cycle at G2/M phase in GSCs. Silencing of stathmin in GSCs also resulted in inhibited the migration/invasion as well as the capability of vasculogenic mimicry. The susceptibility of GSCs to temozolomide was also enhanced by stathmin silencing. Our findings suggest stathmin as a potential target in GSCs for glioma treatment.
authors.affiliation: Department of Neurosurgery, the Fourth Affiliated Hospital of Harbin Medical University, Harbin 150001, China.
authors.forename: Yuwen
authors.initials: Y
authors.lastname: Song
authors.affiliation: Department of Neurosurgery, the Fourth Affiliated Hospital of Harbin Medical University, Harbin 150001, China.
authors.forename: Luyan
authors.initials: L
authors.lastname: Mu
authors.affiliation: Neurosurgery and Vascular Biology Program, Children's Hospital Boston/Harvard Medical School, Boston, MA 02115, USA.
authors.forename: Xuezhe
authors.initials: X
authors.lastname: Han
authors.affiliation: Department of Neurosurgery, the Fourth Affiliated Hospital of Harbin Medical University, Harbin 150001, China lxq1960@hotmail.com.
authors.forename: Xiaoqian
authors.initials: X
authors.lastname: Liu
authors.affiliation: Department of Genetics, Harbin Medical University, Harbin 150081, China.
authors.forename: Songbin
authors.initials: S
authors.lastname: Fu
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology* / Q000627:therapeutic use*; D001483:Base Sequence; D001932:Brain Neoplasms / Q000188:drug therapy* / Q000473:pathology; D045744:Cell Line, Tumor; D017931:DNA Primers; D020868:Gene Silencing; D005910:Glioma / Q000188:drug therapy* / Q000473:pathology; D006801:Humans; D014411:Neoplastic Stem Cells / Q000187:drug effects*; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics*; D060888:Real-Time Polymerase Chain Reaction; D050777:Stathmin / Q000235:genetics / Q000502:physiology*
chemical_list: D000970:Antineoplastic Agents; D017931:DNA Primers; D034741:RNA, Small Interfering; D050777:Stathmin
keywords: glioma stem cells; invasion; stathmin; temozolomide