title: [Metabolome response to tdh expression of pathogenic Vibrio parahaemolyticus induced by different temperatures].
abstract: OBJECTIVE
  We studied the metabolome responses to different relative virulence gene expression of pathogenic Vibrio parahaemolyticus based on reverse transcription real-time PCR (RT-PCR) and metabolomics.
  
  
  METHODS
  We extracted total RNA and metabolites of V. parahaemolyticus ATCC33846 cultured at different temperatures (4, 25 and 37 degrees C). We applied relative quantification to analyze the tdh expression and Ultra Performance Liquid Chromatography & Quadrupole-Time-of-Flight Mass Spectrometry (UPLC/Q-TOF-MS) platform to determine the low-molecular-weight metabolites. Then, we compared the metabolic profiling of V. parahaemolyticus by principal components analysis. The correlation between relative gene expression and metabolome was obtained by Pearson and Spearman correlation coefficien (r) analysis.
  
  
  RESULTS
  The expression level of tdh at different temperatures ranked 25 degrees C > 4 degrees C > 37 degrees C. The mai metabolites that changed significantly (P < 0.05) were organic acids, amino acids, alcohols, ketones, and esters. The results from correlation coefficient suggested that 11 metabolites were highly correlated with tdh expression, including negative correlations and 8 positive correlations, at the threshold of | r | = 1, P < 0.05. Further analysis revealed that alcohols were highly significant correlated with tdh expression.
  
  
  CONCLUSIONS
  The results suggested that there were significant correlation between metabolome and virulence genes expression, which might contribute to understanding the pathogenic mechanism of V. parahaemolyticus.
authors.forename: Zhuoran
authors.initials: Z
authors.lastname: Guo
authors.forename: Xiaowen
authors.initials: X
authors.lastname: Cui
authors.forename: Jingjing
authors.initials: J
authors.lastname: Wang
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Sun
authors.forename: Yingjie
authors.initials: Y
authors.lastname: Pan
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhao
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D006460:Hemolysin Proteins / Q000235:genetics / Q000378:metabolism*; D055442:Metabolome; D013696:Temperature; D014736:Vibrio parahaemolyticus / Q000235:genetics / Q000378:metabolism*; D037521:Virulence Factors / Q000235:genetics / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D006460:Hemolysin Proteins; D037521:Virulence Factors