title: Rapid determination of retinoic acid and its main isomers in plasma by second-order high-performance liquid chromatography data modeling.
abstract: This paper reports the development of a method based on high-performance liquid chromatography (HPLC) coupled to second-order data modeling with multivariate curve resolution-alternating least-squares (MCR-ALS) for quantification of retinoic acid and its main isomers in plasma in only 5.5 min. The compounds retinoic acid (RA), 13-cis-retinoic acid, 9-cis-retinoic acid, and 9,13-di-cis-retinoic acid were partially separated by use of a Poroshell 120 EC-C18 (3.0 mm × 30 mm, 2.7 μm particle size) column. Overlapping not only among the target analytes but also with the plasma interferents was resolved by exploiting the second-order advantage of the multi-way calibration. A validation study led to the following results: trueness with recoveries of 98.5-105.9 % for RA, 95.7-110.1 % for 13-cis-RA, 97.1-110.8 % for 9-cis-RA, and 99.5-110.9 % for 9,13-di-cis-RA; repeatability with RSD of 3.5-3.1 % for RA, 3.5-1.5 % for 13-cis-RA, 4.6-2.7 % for 9-cis-RA, and 5.2-2.7 % for 9,13-di-cis-RA (low and high levels); and intermediate precision (inter-day precision) with RSD of 3.8-3.0 % for RA, 2.9-2.4 % for 13-cis-RA, 3.6-3.2 % for 9,13-di-cis-RA, and 3.2-2.9 % for 9-cis-RA (low and high levels). In addition, a robustness study revealed the method was suitable for monitoring patients with dermatological diseases treated with pharmaceutical products containing RA and 13-cis-RA.
authors.affiliation: Laboratorio de Desarrollo Analítico y Quimiometría (LADAQ), Cátedra de Química Analítica I, Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral-CONICET, Ciudad Universitaria, 3000, Santa Fe, Argentina.
authors.forename: Carla M
authors.initials: CM
authors.lastname: Teglia
authors.forename: María S
authors.initials: MS
authors.lastname: Cámara
authors.forename: Héctor C
authors.initials: HC
authors.lastname: Goicoechea
mesh_terms: D000077556:Alitretinoin; D002851:Chromatography, High Pressure Liquid / Q000191:economics / Q000379:methods*; D006801:Humans; D007536:Isomerism; D015474:Isotretinoin / Q000097:blood* / Q000302:isolation & purification; D057230:Limit of Detection; D013997:Time Factors; D014212:Tretinoin / Q000031:analogs & derivatives* / Q000097:blood / Q000302:isolation & purification
chemical_list: D000077556:Alitretinoin; C105492:9,13-retinoic acid; D014212:Tretinoin; D015474:Isotretinoin