title: Phytochemical screening of five medicinal legumes and their evaluation for in vitro anti-tubercular activity.
abstract: BACKGROUND
  Tuberculosis is a leading cause of death in the world. A new alternative for the treatment of tuberculosis is urgently required, due to the emergence of multidrug resistant Mycobacterium tuberculosis.
  
  
  OBJECTIVE
  There is currently considerable interest in developing potential drugs from medicinal plants for treating tuberculosis. To evaluate anti-tubercular activity in the leaves of Kingiodendron pinnatum Rox. Hams., Humboldtia brunonis Wall., Indigofera cassioides Rottl.ex DC., Derris scandens Benth. and Ceasalpinia mimosoides Lamk.
  
  
  METHODS
  Non-polar and polar solvent extracts of leaves of these medicinal legumes were tested against M. tuberculosis H37RV and minimum inhibitory concentrations (MICs) were determined by the agar based proportion assay.
  
  
  RESULTS
  Phytochemical screening for secondary metabolites revealed the presence of saponins, steroids, anthro-quinones, terpinods, flavonoids and phlabotanins. Crude leaf extracts of these plants have shown MIC value of 50 μg/ml as against the standard drug Isoniazid value of 0.025 μg/ml.
  
  
  CONCLUSIONS
  Results showed that crude extracts of legume leaves screened exhibited potential anti-tubercular activity against M. tuberculosis and further work is required to identify the active molecule of these legumes, to get a novel anti-tubercular drug. This is the maiden finding on anti-tubercular activity of these medicinal legumes.
authors.affiliation: Department of Clinical and Experimental Medicine, University of Linkoping, Sweden, India.
authors.forename: J Komal
authors.initials: JK
authors.lastname: Kumar
authors.affiliation: Department of Environmental Science, University of Mysore, Karnataka, India.
authors.forename: A G
authors.initials: AG
authors.lastname: Devi Prasad
authors.affiliation: Drug Target Discovery and Development Division, Central Drug Research Institute, Lucknow, Uttar Pradesh, India.
authors.forename: Vinita
authors.initials: V
authors.lastname: Chaturvedi
keywords: Bioactive compounds; medicinal legumes; secondary metabolites; tuberculosis