title: Phospholipase A2 group IIA is elevated in endometriomas but not in peritoneal fluid and serum of ovarian endometriosis patients.
abstract: Our previous gene expression analysis identified phospholipase A2 group IIA (PLA2G2A) as a potential biomarker of ovarian endometriosis. The aim of this study was to evaluate PLA2G2A mRNA and protein levels in tissue samples (endometriomas and normal endometrium) and in serum and peritoneal fluid of ovarian endometriosis patients and control women. One-hundred and sixteen women were included in this study: the case group included 70 ovarian endometriosis patients, and the control group included 38 healthy women and 8 patients with benign ovarian cysts. We observed 41.6-fold greater PLA2G2A mRNA levels in endometrioma tissue, compared to normal endometrium tissue. Using Western blotting, PLA2G2A was detected in all samples of endometriomas, but not in normal endometrium, and immunohistochemistry showed PLA2G2A-specific staining in epithelial cells of endometrioma paraffin sections. However, there were no significant differences in PLA2G2A levels between cases and controls according to ELISA of peritoneal fluid (6.0 ± 4.4 ng/ml, 6.6 ± 4.3 ng/ml; p = 0.5240) and serum (2.9 ± 2.1 ng/ml, 3.1 ± 2.2 ng/ml; p = 0.7989). Our data indicate that PLA2G2A is implicated in the pathophysiology of ovarian endometriosis, but that it cannot be used as a diagnostic biomarker.
authors.affiliation: Institute of Biochemistry, Faculty of Medicine, University of Ljubljana , Ljubljana , Slovenia .
authors.forename: Vida
authors.initials: V
authors.lastname: Kocbek
authors.forename: Nick A
authors.initials: NA
authors.lastname: Bersinger
authors.forename: Vesna
authors.initials: V
authors.lastname: Brglez
authors.forename: Michael D
authors.initials: MD
authors.lastname: Mueller
authors.forename: Toni
authors.initials: T
authors.lastname: Petan
authors.forename: Tea Lanišnik
authors.initials: TL
authors.lastname: Rižner
mesh_terms: D000328:Adult; D001202:Ascitic Fluid / Q000378:metabolism*; D016022:Case-Control Studies; D004715:Endometriosis / Q000097:blood / Q000235:genetics / Q000378:metabolism*; D004717:Endometrium / Q000378:metabolism*; D005260:Female; D054501:Group II Phospholipases A2 / Q000097:blood / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D008875:Middle Aged; D010048:Ovarian Cysts / Q000097:blood / Q000235:genetics / Q000378:metabolism; D010049:Ovarian Diseases / Q000097:blood / Q000235:genetics / Q000378:metabolism*; D055815:Young Adult
chemical_list: D054501:Group II Phospholipases A2
keywords: Biomarkers; PLA2G2A; inflammation; ovarian endometriosis