title: Characterization of the binding interaction between the oncoprotein gankyrin and a grafted S6 ATPase.
abstract: A complex with the C-terminal portion of the proteosomal subunit S6 ATPase is the only available structure of a protein-protein interaction involving the oncoprotein gankyrin. However, difficulties associated with recombinant expression of S6 ATPase alone, or truncations thereof, have limited our understanding of this assembly. We replaced the C-terminal portion of FtsH from Escherichia coli with the structurally homologous C-terminal portion of S6 ATPase and used this grafted protein to characterize the gankyrin-S6 ATPase binding interaction by isothermal titration calorimetry.
authors.affiliation: Department of Chemistry and ‡Department of Biochemistry & Molecular Biology, Colorado State University , Fort Collins, Colorado 80523, United States.
authors.forename: Alex M
authors.initials: AM
authors.lastname: Chapman
authors.forename: Bryce E
authors.initials: BE
authors.lastname: Rogers
authors.forename: Brian R
authors.initials: BR
authors.lastname: McNaughton
mesh_terms: D049069:ATP-Dependent Proteases / Q000737:chemistry; D000251:Adenosine Triphosphatases / Q000737:chemistry*; D002151:Calorimetry; D020134:Catalytic Domain; D004926:Escherichia coli; D029968:Escherichia coli Proteins / Q000737:chemistry; D006801:Humans; D046988:Proteasome Endopeptidase Complex / Q000737:chemistry*; D011485:Protein Binding; D054730:Protein Interaction Domains and Motifs; D021122:Protein Subunits / Q000737:chemistry; D011518:Proto-Oncogene Proteins / Q000737:chemistry*; D011993:Recombinant Fusion Proteins / Q000737:chemistry; D013816:Thermodynamics; D017186:Titrimetry
chemical_list: D029968:Escherichia coli Proteins; C403591:PSMD10 protein, human; D021122:Protein Subunits; D011518:Proto-Oncogene Proteins; D011993:Recombinant Fusion Proteins; D049069:ATP-Dependent Proteases; C513383:FtsH protein, E coli; D046988:Proteasome Endopeptidase Complex; D000251:Adenosine Triphosphatases