title: Note: A micro-perfusion system for use during real-time physiological studies under high pressure.
abstract: We construct a micro-perfusion system using piston screw pump generators for use during real-time, high-pressure physiological studies. Perfusion is achieved using two generators, with one generator being compressed while the other is retracted, thus maintaining pressurization while producing fluid flow. We demonstrate control over perfusion rates in the 10-μl/s range and the ability to change between fluid reservoirs at up to 50 MPa. We validate the screw-pump approach by monitoring the cyanide-induced response of UV-excited autofluorescence from Saccharomyces cerevisiae under pressurization.
authors.affiliation: Department of Physics, Miami University, Oxford, Ohio 45056, USA.
authors.forename: Jeff
authors.initials: J
authors.lastname: Maltas
authors.affiliation: Department of Physics, Miami University, Oxford, Ohio 45056, USA.
authors.forename: Zac
authors.initials: Z
authors.lastname: Long
authors.affiliation: Department of Physics, Miami University, Oxford, Ohio 45056, USA.
authors.forename: Alison
authors.initials: A
authors.lastname: Huff
authors.affiliation: Department of Physics, Miami University, Oxford, Ohio 45056, USA.
authors.forename: Ryan
authors.initials: R
authors.lastname: Maloney
authors.affiliation: Department of Physics, Miami University, Oxford, Ohio 45056, USA.
authors.forename: Jordan
authors.initials: J
authors.lastname: Ryan
authors.affiliation: Department of Physics, Miami University, Oxford, Ohio 45056, USA.
authors.forename: Paul
authors.initials: P
authors.lastname: Urayama
mesh_terms: D003486:Cyanides / Q000494:pharmacology; D004867:Equipment Design; D055616:Microtechnology / Q000295:instrumentation*; D010477:Perfusion / Q000295:instrumentation*; D011312:Pressure; D012441:Saccharomyces cerevisiae / Q000737:chemistry / Q000187:drug effects; D013050:Spectrometry, Fluorescence; D013997:Time Factors
chemical_list: D003486:Cyanides