title: Participation of the Salmonella OmpD porin in the infection of RAW264.7 macrophages and BALB/c mice.
abstract: Salmonella Typhimurium is the etiological agent of gastroenteritis in humans and enteric fever in mice. Inside these hosts, Salmonella must overcome hostile conditions to develop a successful infection, a process in which the levels of porins may be critical. Herein, the role of the Salmonella Typhimurium porin OmpD in the infection process was assessed for adherence, invasion and proliferation in RAW264.7 mouse macrophages and in BALB/c mice. In cultured macrophages, a ΔompD strain exhibited increased invasion and proliferation phenotypes as compared to its parental strain. In contrast, overexpression of ompD caused a reduction in bacterial proliferation but did not affect adherence or invasion. In the murine model, the ΔompD strain showed increased ability to survive and replicate in target organs of infection. The ompD transcript levels showed a down-regulation when Salmonella resided within cultured macrophages and when it colonized target organs in infected mice. Additionally, cultured macrophages infected with the ΔompD strain produced lower levels of reactive oxygen species, suggesting that down-regulation of ompD could favor replication of Salmonella inside macrophages and the subsequent systemic dissemination, by limiting the reactive oxygen species response of the host.
authors.affiliation: Laboratorio de Microbiología Molecular, Departamento de Ciencias Biológicas, Facultad de Ciencias Biológicas, Universidad Andres Bello, Santiago, Chile.
authors.forename: Francisco
authors.initials: F
authors.lastname: Ipinza
authors.affiliation: Laboratorio de Microbiología Molecular, Departamento de Ciencias Biológicas, Facultad de Ciencias Biológicas, Universidad Andres Bello, Santiago, Chile.
authors.forename: Bernardo
authors.initials: B
authors.lastname: Collao
authors.affiliation: Laboratorio de Microbiología Molecular, Departamento de Ciencias Biológicas, Facultad de Ciencias Biológicas, Universidad Andres Bello, Santiago, Chile.
authors.forename: Debbie
authors.initials: D
authors.lastname: Monsalva
authors.affiliation: Departamento de Microbiología Molecular, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, México.
authors.forename: Victor H
authors.initials: VH
authors.lastname: Bustamante
authors.affiliation: Laboratorio de Microbiología Molecular, Departamento de Ciencias Biológicas, Facultad de Ciencias Biológicas, Universidad Andres Bello, Santiago, Chile.
authors.forename: Roberto
authors.initials: R
authors.lastname: Luraschi
authors.affiliation: Center for Bioinformatics and Integrative Biology, Facultad de Ciencias Biologicas, Universidad Andres Bello, Santiago, Chile.
authors.forename: Melissa
authors.initials: M
authors.lastname: Alegría-Arcos
authors.affiliation: Center for Bioinformatics and Integrative Biology, Facultad de Ciencias Biologicas, Universidad Andres Bello, Santiago, Chile.
authors.forename: Daniel E
authors.initials: DE
authors.lastname: Almonacid
authors.affiliation: Center for Bioinformatics and Integrative Biology, Facultad de Ciencias Biologicas, Universidad Andres Bello, Santiago, Chile.
authors.forename: Daniel
authors.initials: D
authors.lastname: Aguayo
authors.affiliation: Laboratorio de Microbiología Molecular, Departamento de Ciencias Biológicas, Facultad de Ciencias Biológicas, Universidad Andres Bello, Santiago, Chile.
authors.forename: Iván L
authors.initials: IL
authors.lastname: Calderón
authors.affiliation: Laboratorio de Microbiología Molecular, Departamento de Ciencias Biológicas, Facultad de Ciencias Biológicas, Universidad Andres Bello, Santiago, Chile.
authors.forename: Fernando
authors.initials: F
authors.lastname: Gil
authors.affiliation: Laboratorio de Microbiología, Departamento de Bioquímica y Biología Molecular, Facultad de Ciencias Químicas y Farmacéuticas, Universidad de Chile, Santiago, Chile.
authors.forename: Carlos A
authors.initials: CA
authors.lastname: Santiviago
authors.affiliation: Great Lakes Bioenergy Research Center and Department of Biomolecular Chemistry, University of Wisconsin-Madison, Madison, Wisconsin, United States of America.
authors.forename: Eduardo H
authors.initials: EH
authors.lastname: Morales
authors.affiliation: Departamento de Microbiología Molecular, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, México.
authors.forename: Edmundo
authors.initials: E
authors.lastname: Calva
authors.affiliation: Laboratorio de Microbiología Molecular, Departamento de Ciencias Biológicas, Facultad de Ciencias Biológicas, Universidad Andres Bello, Santiago, Chile.
authors.forename: Claudia P
authors.initials: CP
authors.lastname: Saavedra
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D015536:Down-Regulation; D004926:Escherichia coli / Q000378:metabolism; D005260:Female; D054884:Host-Pathogen Interactions; D008264:Macrophages / Q000378:metabolism* / Q000382:microbiology; D008807:Mice, Inbred BALB C; D008958:Models, Molecular; D009154:Mutation; D018272:Porins / Q000235:genetics / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000378:metabolism; D012481:Salmonella Infections, Animal / Q000378:metabolism* / Q000382:microbiology; D012486:Salmonella typhimurium / Q000472:pathogenicity / Q000502:physiology*
chemical_list: D001426:Bacterial Proteins; D018272:Porins; D017382:Reactive Oxygen Species