title: Depth resolved hyperspectral imaging spectrometer based on structured light illumination and Fourier transform interferometry.
abstract: A depth resolved hyperspectral imaging spectrometer can provide depth resolved imaging both in the spatial and the spectral domain. Images acquired through a standard imaging Fourier transform spectrometer do not have the depth-resolution. By post processing the spectral cubes (x, y, λ) obtained through a Sagnac interferometer under uniform illumination and structured illumination, spectrally resolved images with depth resolution can be recovered using structured light illumination algorithms such as the HiLo method. The proposed scheme is validated with in vitro specimens including fluorescent solution and fluorescent beads with known spectra. The system is further demonstrated in quantifying spectra from 3D resolved features in biological specimens. The system has demonstrated depth resolution of 1.8 μm and spectral resolution of 7 nm respectively.
authors.affiliation: Department of Mechanical Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
authors.forename: Heejin
authors.initials: H
authors.lastname: Choi
authors.affiliation: BioSym, Singapore-MIT Alliance for Research and Technology, Singapore 138602, Singapore ; Department of Biological Sciences, National University of Singapore, Singapore 138602, Singapore.
authors.forename: Dushan
authors.initials: D
authors.lastname: Wadduwage
authors.affiliation: Department of Biological Sciences, National University of Singapore, Singapore 138602, Singapore.
authors.forename: Paul T
authors.initials: PT
authors.lastname: Matsudaira
authors.affiliation: Department of Mechanical Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA ; Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA ; BioSym, Singapore-MIT Alliance for Research and Technology, Singapore 138602, Singapore ; Laser Biomedical Research Center, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
authors.forename: Peter T C
authors.initials: PT
authors.lastname: So
keywords: (170.2520) Fluorescence microscopy; (170.6900) Three-dimensional microscopy; (300.6300) Spectroscopy, Fourier transforms