title: Direct Interaction between Ras Homolog Enriched in Brain and FK506 Binding Protein 38 in Cashmere Goat Fetal Fibroblast Cells.
abstract: Ras homolog enriched in brain (Rheb) and FK506 binding protein 38 (FKBP38) are two important regulatory proteins in the mammalian target of rapamycin (mTOR) pathway. There are contradictory data on the interaction between Rheb and FKBP38 in human cells, but this association has not been examined in cashmere goat cells. To investigate the interaction between Rheb and FKBP38, we overexpressed goat Rheb and FKBP38 in goat fetal fibroblasts, extracted whole proteins, and performed coimmunoprecipitation to detect them by western blot. We found Rheb binds directly to FKBP38. Then, we constructed bait vectors (pGBKT7-Rheb/FKBP38) and prey vectors (pGADT7-Rheb/FKBP38), and examined their interaction by yeast two-hybrid assay. Their direct interaction was observed, regardless of which plasmid served as the prey or bait vector. These results indicate that the 2 proteins interact directly in vivo. Novel evidence is presented on the mTOR signal pathway in Cashmere goat cells.
authors.affiliation: Chifeng Municipal Hospital, Chifeng, Inner Mongolia 024000, China .
authors.forename: Xiaojing
authors.initials: X
authors.lastname: Wang
authors.affiliation: Chifeng Municipal Hospital, Chifeng, Inner Mongolia 024000, China .
authors.forename: Yanfeng
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Chifeng Municipal Hospital, Chifeng, Inner Mongolia 024000, China .
authors.forename: Xu
authors.initials: X
authors.lastname: Zheng
authors.affiliation: Chifeng Municipal Hospital, Chifeng, Inner Mongolia 024000, China .
authors.forename: Xiyan
authors.initials: X
authors.lastname: Hao
authors.affiliation: Chifeng Municipal Hospital, Chifeng, Inner Mongolia 024000, China .
authors.forename: Yan
authors.initials: Y
authors.lastname: Liang
authors.affiliation: Chifeng Municipal Hospital, Chifeng, Inner Mongolia 024000, China .
authors.forename: Manlin
authors.initials: M
authors.lastname: Wu
authors.affiliation: Chifeng Municipal Hospital, Chifeng, Inner Mongolia 024000, China .
authors.forename: Xiao
authors.initials: X
authors.lastname: Wang
authors.affiliation: Chifeng Municipal Hospital, Chifeng, Inner Mongolia 024000, China .
authors.forename: Zhigang
authors.initials: Z
authors.lastname: Wang
keywords: FK506 Binding Protein 38 [FKBP38]; Interaction; Ras homolog enriched in brain [Rheb]; mammalian Target of Rapamycin [mTOR]