title: Novel IL-6-secreting γδT cells increased in patients with atherosclerotic cerebral infarction.
abstract: Mounting evidence has suggested that inflammation associated with interleukin (IL)‑6 and T‑helper (Th)17 cells, has a role in the development of atherosclerotic cerebral infarction (ACI). However, it remains unclear which population of cells determines the levels of IL‑6, and the role of IL‑6‑secreting cells in inducing Th17 cell production. In the present study, IL‑6 levels were determined in patients with ACI, by ELISA. The percentage of CD3+T, CD4+T, CD8+T, CD11c+ dendritic cells and γδT cells were determined by flow cytometry, and the correlation between cytokine IL‑6 and γδT cells was determined by statistical analysis. An in vitro culture assay was used to determine whether γδT cells secreted high levels of IL‑6, and induced production of Th17 cells. The patients with ACI had significantly higher levels of IL‑6 and γδT cells. Furthermore, γδT cells were associated with the secretion of a high level of IL‑6 in patients with ACI. These results indicate that γδT cells are novel IL‑6‑secreting cells, which from then on were known as γδT6 cells. In addition, the novel γδT6 cells induced Th17‑cell production, and this induction was dependent on IL‑6. Novel γδT6 cells increased the induction of Th17‑cell production in patients with ACI. The results of the present study suggest that novel γδT6 cells may be a target for strategic therapies of ACI.
authors.affiliation: Department of Neurology, Beijing Chaoyang Hospital, Capital Medical University, Beijing 100020, P.R. China.
authors.forename: Youdi
authors.initials: Y
authors.lastname: He
authors.affiliation: Laboratory of Immunology, Institute of Basic Medical Sciences, Beijing 100850, P.R. China.
authors.forename: Ning
authors.initials: N
authors.lastname: Ma
authors.affiliation: Laboratory of Immunology, Institute of Basic Medical Sciences, Beijing 100850, P.R. China.
authors.forename: Chen
authors.initials: C
authors.lastname: Xing
authors.affiliation: Laboratory of Immunology, Institute of Basic Medical Sciences, Beijing 100850, P.R. China.
authors.forename: Xiaoqian
authors.initials: X
authors.lastname: Wang
authors.affiliation: Laboratory of Immunology, Institute of Basic Medical Sciences, Beijing 100850, P.R. China.
authors.forename: He
authors.initials: H
authors.lastname: Xiao
authors.affiliation: Laboratory of Immunology, Institute of Basic Medical Sciences, Beijing 100850, P.R. China.
authors.forename: Mingke
authors.initials: M
authors.lastname: Zheng
authors.affiliation: Laboratory of Immunology, Institute of Basic Medical Sciences, Beijing 100850, P.R. China.
authors.forename: Gencheng
authors.initials: G
authors.lastname: Han
authors.affiliation: Laboratory of Immunology, Institute of Basic Medical Sciences, Beijing 100850, P.R. China.
authors.forename: Guojiang
authors.initials: G
authors.lastname: Chen
authors.affiliation: Laboratory of Immunology, Institute of Basic Medical Sciences, Beijing 100850, P.R. China.
authors.forename: Chunmei
authors.initials: C
authors.lastname: Hou
authors.affiliation: Laboratory of Immunology, Institute of Basic Medical Sciences, Beijing 100850, P.R. China.
authors.forename: Beifen
authors.initials: B
authors.lastname: Shen
authors.affiliation: Laboratory of Immunology, Institute of Basic Medical Sciences, Beijing 100850, P.R. China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Rheumatology, First Hospital of Jilin University, Changchun, Jilin 130021, P.R. China.
authors.forename: Zhenyu
authors.initials: Z
authors.lastname: Jiang
authors.affiliation: Laboratory of Immunology, Institute of Basic Medical Sciences, Beijing 100850, P.R. China.
authors.forename: Renxi
authors.initials: R
authors.lastname: Wang
authors.affiliation: Department of Neurology, Beijing Chaoyang Hospital, Capital Medical University, Beijing 100020, P.R. China.
authors.forename: Wenli
authors.initials: W
authors.lastname: Hu
mesh_terms: D000328:Adult; D000368:Aged; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D002544:Cerebral Infarction / Q000276:immunology / Q000378:metabolism / Q000473:pathology*; D018920:Coculture Techniques; D003713:Dendritic Cells / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D015850:Interleukin-6 / Q000032:analysis / Q000378:metabolism*; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000378:metabolism; D016176:T-Lymphocyte Subsets / Q000166:cytology / Q000378:metabolism*; D058504:Th17 Cells / Q000166:cytology / Q000378:metabolism
chemical_list: D015850:Interleukin-6; D016692:Receptors, Antigen, T-Cell, gamma-delta