title: Denitrification of groundwater using a sulfur-oxidizing autotrophic denitrifying anaerobic fluidized-bed MBR: performance and bacterial community structure.
abstract: This paper investigates a novel sulfur-oxidizing autotrophic denitrifying anaerobic fluidized bed membrane bioreactor (AnFB-MBR) that has the potential to overcome the limitations of conventional sulfur-oxidizing autotrophic denitrification systems. The AnFB-MBR produced consistent high-quality product water when fed by a synthetic groundwater with NO3 (-)-N ranging 25-80 mg/L and operated at hydraulic retention times of 0.5-5.0 h. A nitrate removal rate of up to 4.0 g NO3 (-)-N/Lreactord was attained by the bioreactor, which exceeded any reported removal capacity. The flux of AnFB-MBR was maintained in the range of 1.5-15 L m(-2) h(-1). Successful membrane cleaning was practiced with cleaning cycles of 35-81 days, which had no obvious effect on the AnFB-MBR performance. The (15) N-tracer analyses elucidated that nitrogen was converted into (15) N2-N and (15) N-biomass accounting for 88.1-93.1 % and 6.4-11.6 % of the total nitrogen produced, respectively. Only 0.3-0.5 % of removed nitrogen was in form of (15)N2O-N in sulfur-oxidizing autotrophic denitrification process, reducing potential risks of a significant amount of N2O emissions. The sulfur-oxidizing autotrophic denitrifying bacterial consortium was composed mainly of bacteria from Proteobacteria, Chlorobi, and Chloroflexi phyla, with genera Thiobacillus, Sulfurimonas, and Ignavibacteriales dominating the consortium. The pyrosequencing assays also suggested that the stable microbial communities corresponded to the elevated performance of the AnFB-MBR. Overall, this research described relatively high nitrate removal, acceptable flux, indicating future potential for the technology in practice.
authors.affiliation: Key Laboratory of Drinking Water Science and Technology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, 100085, China.
authors.forename: Lili
authors.initials: L
authors.lastname: Zhang
authors.forename: Chao
authors.initials: C
authors.lastname: Zhang
authors.forename: Chengzhi
authors.initials: C
authors.lastname: Hu
authors.forename: Huijuan
authors.initials: H
authors.lastname: Liu
authors.forename: Jiuhui
authors.initials: J
authors.lastname: Qu
mesh_terms: D052818:Autotrophic Processes; D001419:Bacteria / Q000145:classification / Q000378:metabolism*; D018533:Biomass; D019149:Bioreactors; D004269:DNA, Bacterial / Q000235:genetics; D058440:Denitrification; D020565:Epsilonproteobacteria / Q000145:classification / Q000378:metabolism; D060587:Groundwater / Q000382:microbiology*; D009566:Nitrates / Q000737:chemistry; D009584:Nitrogen / Q000737:chemistry; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D013455:Sulfur / Q000737:chemistry*; D013855:Thiobacillus / Q000145:classification / Q000378:metabolism
chemical_list: D004269:DNA, Bacterial; D009566:Nitrates; D012336:RNA, Ribosomal, 16S; D013455:Sulfur; D009584:Nitrogen