title: Effects of co-treatment with sulforaphane and autophagy modulators on uridine 5'-diphospho-glucuronosyltransferase 1A isoforms and cytochrome P450 3A4 expression in Caco-2 human colon cancer cells.
abstract: Sulforaphane (SFN), which is highly enriched in cruciferous vegetables, has been investigated for its cancer chemopreventive properties and ability to induce autophagy. Uridine 5'-diphospho (UDP)-glucuronosyltransferase (UGT)1A induction is one of the mechanisms that is responsible for the cancer chemopreventive activity of SFN. The current study demonstrates that rapamycin may enhance the chemopreventive effects of SFN on Caco-2 cells; this may be partially attributed to nuclear translocation of nuclear factor erythroid 2-related factor 2 (Nrf2)- and human pregnane X receptor (hPXR)-mediated UGT1A1, UGT1A8 and UGT1A10 induction. These results indicate that targeting autophagy modulation may be a promising strategy for increasing the chemopreventive effects of SFN in cases of colon cancer.
authors.affiliation: Department of Geriatrics and Gastroenterology, Qi-Lu Hospital of Shandong University, Key Laboratory of Proteomics of Shandong, Jinan, Shandong 250012, P.R. China.
authors.forename: Min
authors.initials: M
authors.lastname: Wang
authors.affiliation: Department of Gastroenterology, Jinan Central Hospital, Shandong University, Jinan, Shandong 250013, P.R. China.
authors.forename: Jing-Yu
authors.initials: JY
authors.lastname: Zhu
authors.affiliation: Department of Gastroenterology, China-Japan Friendship Hospital, Beijing 100029, P.R. China ; Graduate School, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing 100029, P.R. China.
authors.forename: Shuo
authors.initials: S
authors.lastname: Chen
authors.affiliation: Department of Geriatrics and Gastroenterology, Qi-Lu Hospital of Shandong University, Key Laboratory of Proteomics of Shandong, Jinan, Shandong 250012, P.R. China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Qing
authors.affiliation: Department of General Surgery, Qi-Lu Hospital of Shandong University, Jinan, Shandong 250012, P.R. China.
authors.forename: Dong
authors.initials: D
authors.lastname: Wu
authors.affiliation: Department of Geriatrics and Gastroenterology, Qi-Lu Hospital of Shandong University, Key Laboratory of Proteomics of Shandong, Jinan, Shandong 250012, P.R. China.
authors.forename: Ying-Min
authors.initials: YM
authors.lastname: Lin
authors.affiliation: School of Medicine, Shanghai Jiao Tong University, Shanghai 200030, P.R. China.
authors.forename: Ji-Zhuang
authors.initials: JZ
authors.lastname: Luo
authors.affiliation: Department of Gastroenterology, Qi-Lu Hospital of Shandong University, Jinan, Shandong 250012, P.R. China.
authors.forename: Wei
authors.initials: W
authors.lastname: Han
authors.affiliation: Department of Gastroenterology, Qi-Lu Hospital of Shandong University, Jinan, Shandong 250012, P.R. China.
authors.forename: Yan-Qing
authors.initials: YQ
authors.lastname: Li
keywords: Caco-2 cells; autophagy; chemoprevention; cytochrome P450 3A4; sulforaphane; uridine 5′-diphospho-glucuronosyltransferase 1A