title: Qualitative and quantitative analysis of the major constituents in Acorus tatarinowii Schott by HPLC/ESI-QTOF-MS/MS.
abstract: Acorus tatarinowii Schott (ATS) is a well-known traditional Chinese medicine (TCM) for the treatment of epilepsy, amnesia and insomnia. In this study, a methodology utilizing high-performance liquid chromatography (HPLC) coupled with electrospray ionization quadrupole time-of-flight mass spectrometry (ESI-QTOF-MS/MS) was established for the separation and structural identification of the major chemical constituents in ATS for the first time. Overall, 46 major constituents including flavonoid glycosides, phenylpropane derivatives, amides and lignans were identified or tentatively characterized. Seven major constituents, including four phenylpropane derivatives and three lignans, were further quantified as marker substances, which showed good linearity within the test ranges. These results indicated that the developed quantitative method was linear, sensitive, and precise for quality control of ATS.
authors.affiliation: Shanghai Institute of MateriaMedica, Chinese Academy of Sciences, Shanghai, 201203, People's Republic of China.
authors.forename: Feng
authors.initials: F
authors.lastname: Zhang
authors.affiliation: Shanghai Institute of MateriaMedica, Chinese Academy of Sciences, Shanghai, 201203, People's Republic of China.
authors.forename: Peng
authors.initials: P
authors.lastname: Qi
authors.affiliation: Shanghai Institute of MateriaMedica, Chinese Academy of Sciences, Shanghai, 201203, People's Republic of China.
authors.forename: Rui
authors.initials: R
authors.lastname: Xue
authors.affiliation: Shanghai Institute of MateriaMedica, Chinese Academy of Sciences, Shanghai, 201203, People's Republic of China.
authors.forename: Zhixiong
authors.initials: Z
authors.lastname: Li
authors.affiliation: Shanghai Institute of MateriaMedica, Chinese Academy of Sciences, Shanghai, 201203, People's Republic of China.
authors.forename: Kaicheng
authors.initials: K
authors.lastname: Zhu
authors.affiliation: Life and Environment Science College, Shanghai Normal University, Shanghai, 200234, People's Republic of China.
authors.forename: Ping
authors.initials: P
authors.lastname: Wan
authors.affiliation: Shanghai Institute of MateriaMedica, Chinese Academy of Sciences, Shanghai, 201203, People's Republic of China.
authors.forename: Chenggang
authors.initials: C
authors.lastname: Huang
mesh_terms: D029023:Acorus / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D005419:Flavonoids / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification; D006027:Glycosides / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification; D017705:Lignans / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification; D057230:Limit of Detection; D016014:Linear Models; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D004365:Drugs, Chinese Herbal; D005419:Flavonoids; D006027:Glycosides; D017705:Lignans
keywords: Acorus tatarinowii Schott; HPLC/ESI-QTOF-MS/MS; identification; quantification