title: Proteomic investigation of male Gammarus fossarum, a freshwater crustacean, in response to endocrine disruptors.
abstract: While the decrease in human sperm count in response to pollutants is a worldwide concern, little attention is being devoted to its causes and occurrence in the biodiversity of the animal kingdom. Arthropoda is the most species-rich phyla, inhabiting all aquatic and terrestrial ecosystems. During evolution, key molecular players of the arthropod endocrine system have diverged from the vertebrate counterparts. Consequently, arthropods may have different sensitivities toward endocrine disrupting chemicals (EDCs). Here alteration of sperm quality in a crustacean, Gammarus fossarum, a popular organism in freshwater risk assessment, was investigated after laboratory exposure to various concentrations of three different xenobiotics: cadmium, methoxyfenozide, and pyriproxyfen. The integrity of the reproductive process was assessed by means of sperm-quality markers. For each substance, semiquantitative/relative proteomics based on spectral counting procedure was carried out on male gonads to observe the biological impact. The changes in a total of 871 proteins were monitored in response to toxic pressure. A drastic effect was observed on spermatozoon production, with a dose-response relationship. While exposure to EDCs leads to strong modulations of male-specific proteins in testis, no induction of female-specific proteins was noted. Also, a significant portion of orphans proved to be sensitive to toxic stress.
authors.affiliation: Irstea, Unité de Recherche MALY, Laboratoire d'écotoxicologie, CS70077, F-69626 Villeurbanne, France.
authors.forename: Judith
authors.initials: J
authors.lastname: Trapp
authors.forename: Jean
authors.initials: J
authors.lastname: Armengaud
authors.forename: Olivier
authors.initials: O
authors.lastname: Pible
authors.forename: Jean-Charles
authors.initials: JC
authors.lastname: Gaillard
authors.forename: Khedidja
authors.initials: K
authors.lastname: Abbaci
authors.forename: Yassine
authors.initials: Y
authors.lastname: Habtoul
authors.forename: Arnaud
authors.initials: A
authors.lastname: Chaumot
authors.forename: Olivier
authors.initials: O
authors.lastname: Geffard
mesh_terms: D033304:Amphipoda / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D002104:Cadmium; D004305:Dose-Response Relationship, Drug; D052244:Endocrine Disruptors / Q000633:toxicity*; D005786:Gene Expression Regulation / Q000187:drug effects*; D006834:Hydrazines; D007605:Juvenile Hormones; D008297:Male; D020543:Proteome; D040901:Proteomics / Q000379:methods*; D011725:Pyridines; D012737:Sex Factors; D013045:Species Specificity; D013094:Spermatozoa / Q000187:drug effects; D015262:Xenobiotics / Q000633:toxicity*
chemical_list: D052244:Endocrine Disruptors; D006834:Hydrazines; D007605:Juvenile Hormones; D020543:Proteome; D011725:Pyridines; D015262:Xenobiotics; D002104:Cadmium; C055613:pyriproxyfen; C406712:methoxyfenozide
keywords: crustacean; ecotoxicology; endocrine disruption; expression profile; reproduction; shotgun proteomics