title: Evaluating Antiproliferative and Antioxidant Activity of Marrubium crassidens.
abstract: OBJECTIVE
  Naturally occurring substances as novel drugs in cancer therapy, at all times, represent a challenge to science since medicinal plants are proving to be brilliant sources of new chemopreventive agents.
  
  
  METHODS
  In the present study, methanol extract from aerial parts of Marrubium crassidens was assessed for its antiproliferative activity in the breast cancer cell line MCF-7 through MTT bioassay using cell viability and cytotoxicity indices. The antioxidant property of M. crassidens extract together with its phenolic and flavonoids content were evaluated, as well.
  
  
  RESULTS
  According to data obtained in the study, M. crassidens exhibited antiproliferative activity with a gradual rise in cytotoxicty effect setting out on 240µg/mL concentration of the extract. Moreover, the RC50 value for antioxidant activity of the extract was determined as 40µg/mL and values for the total phenolic and flavonoids were calculated as 512.64mg gallic acid equivalent and 212.73mg quercetin equivalent per 100g of dry plant material.
  
  
  CONCLUSIONS
  Generally, the observed antiproliferative and antioxidant properties of M. crassidens could be certified to the high amounts of phenolic and flavonoid content detected in the extract.
authors.affiliation: Students' Research Committee, Faculty of Pharmacy, Tabriz University of Medical Sciences, Iran. ; Research Center for Pharmaceutical Nanotechnology, Faculty of Pharmacy, Tabriz University of Medical Sciences, Iran.
authors.forename: Sanaz
authors.initials: S
authors.lastname: Hamedeyazdan
authors.affiliation: Students' Research Committee, Faculty of Pharmacy, Tabriz University of Medical Sciences, Iran. ; Research Center for Pharmaceutical Nanotechnology, Faculty of Pharmacy, Tabriz University of Medical Sciences, Iran.
authors.forename: Simin
authors.initials: S
authors.lastname: Sharifi
authors.affiliation: Research Center for Pharmaceutical Nanotechnology, Faculty of Pharmacy, Tabriz University of Medical Sciences, Iran.
authors.forename: Hossein
authors.initials: H
authors.lastname: Nazemiyeh
authors.affiliation: Department of Pharmacognosy, Faculty of Pharmacy, Tabriz University of Medical Sciences, Iran.
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Fathiazad
keywords: Free radical scavenger; Lamiaceae; MCF-7 cell line; MTT assay; Marrubium crassidens; Phenols