title: Hierarchically ordered nanopatterns for spatial control of biomolecules.
abstract: The development and study of a benchtop, high-throughput, and inexpensive fabrication strategy to obtain hierarchical patterns of biomolecules with sub-50 nm resolution is presented. A diblock copolymer of polystyrene-b-poly(ethylene oxide), PS-b-PEO, is synthesized with biotin capping the PEO block and 4-bromostyrene copolymerized within the polystyrene block at 5 wt %. These two handles allow thin films of the block copolymer to be postfunctionalized with biotinylated biomolecules of interest and to obtain micropatterns of nanoscale-ordered films via photolithography. The design of this single polymer further allows access to two distinct superficial nanopatterns (lines and dots), where the PEO cylinders are oriented parallel or perpendicular to the substrate. Moreover, we present a strategy to obtain hierarchical mixed morphologies: a thin-film coating of cylinders both parallel and perpendicular to the substrate can be obtained by tuning the solvent annealing and irradiation conditions.
authors.affiliation: Department of Chemistry, Columbia University , New York, New York 10027, United States.
authors.forename: Helen
authors.initials: H
authors.lastname: Tran
authors.forename: Kacey
authors.initials: K
authors.lastname: Ronaldson
authors.forename: Nevette A
authors.initials: NA
authors.lastname: Bailey
authors.forename: Nathaniel A
authors.initials: NA
authors.lastname: Lynd
authors.forename: Kato L
authors.initials: KL
authors.lastname: Killops
authors.forename: Gordana
authors.initials: G
authors.lastname: Vunjak-Novakovic
authors.forename: Luis M
authors.initials: LM
authors.lastname: Campos
mesh_terms: D002478:Cells, Cultured; D006801:Humans; D018625:Microscopy, Atomic Force; D036103:Nanotechnology; D011137:Polystyrenes / Q000737:chemistry; D011506:Proteins / Q000737:chemistry
chemical_list: D011137:Polystyrenes; D011506:Proteins
keywords: block copolymer self-assembly; dual protein patterning; hierarchical patterns