title: Cytotoxicity and gene expression in sarcoma 180 cells in response to spiky magnetoplasmonic supraparticles.
abstract: Multifunctional nanoparticles (NPs) have been designed for a variety of cell imaging and therapeutic applications, and the study of their cellular interactions is crucial to the development of more efficient biomedical applications. Among current nanomaterials, concave core-shell NPs with complex angled geometries are attractive owing to their unique shape-dependent optical and physical properties as well as different tendency for cell interaction. In this study, we investigated the morphology effect of spiky gold-coated iron oxide supraparticles (Fe3O4@Au SPs) on cytotoxicity and global gene expression in sarcoma 180 cells. Cells treated for 7 days with spiky supraparticles (SPs) at concentrations up to 50 μg/mL showed >90% viability, indicating that these NPs were nontoxic. To shed light on the differences in cytotoxicity, we monitored the expression of 33,315 genes using microarray analysis of SP-treated cells. The 171 up-regulated genes and 181 down-regulated genes in spiky SP-treated cells included Il1b, Spp1, Il18, Rbp4, and Il11ra1, where these genes are mainly involved in cell proliferation, differentiation, and apoptosis. These results suggested that the spiky Fe3O4@Au SPs can induce noncytotoxicity and gene expression in tumor cells, which may be a promising cornerstone on which to base related research such as cyto-/genotoxicology of nanomaterials or the design of nanoscale drug carriers.
authors.affiliation: Department of Nano Fusion, and Cogno-Mechatronics Engineering, Pusan National University , Busan 609-735, Republic of Korea.
authors.forename: Hongjian
authors.initials: H
authors.lastname: Zhou
authors.forename: Sun Il
authors.initials: SI
authors.lastname: Choi
authors.forename: Fengming
authors.initials: F
authors.lastname: Zou
authors.forename: Sangjin
authors.initials: S
authors.lastname: Oh
authors.forename: Ji Eun
authors.initials: JE
authors.lastname: Kim
authors.forename: Dae Youn
authors.initials: DY
authors.lastname: Hwang
authors.forename: Jaebeom
authors.initials: J
authors.lastname: Lee
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004337:Drug Carriers / Q000009:adverse effects* / Q000737:chemistry; D005290:Ferric Compounds / Q000737:chemistry; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006046:Gold / Q000737:chemistry; D006801:Humans; D053768:Metal Nanoparticles / Q000009:adverse effects / Q000627:therapeutic use; D009363:Neoplasm Proteins / Q000096:biosynthesis*; D012510:Sarcoma 180 / Q000188:drug therapy / Q000235:genetics*
chemical_list: D004337:Drug Carriers; D005290:Ferric Compounds; D009363:Neoplasm Proteins; C000499:ferric oxide; D006046:Gold
keywords: concave core−shell nanoparticle; cytotoxicity; gene expression; microarray; tumor cell