title: Isolation of clathrin-coated vesicles from tissue culture cells.
abstract: The study of clathrin-coated vesicles (CCVs) isolated from various organs has revealed the identities and important features of many of the factors involved in membrane trafficking. The development of isolation methods using cultured cell lines has made it possible to manipulate the source material before isolation to ask important questions about the roles of these factors and the pathways in which they are involved. We discuss here the advantages and limitations of the use of cultured cell lines for the isolation of CCVs.
authors.affiliation: Department of Proteomics and Signal Transduction, Max Plank Institute of Biochemistry, 82152 Martinsried, Germany;
authors.forename: Georg H H
authors.initials: GH
authors.lastname: Borner
authors.affiliation: Department of Cellular and Molecular Physiology, Institute of Translational Medicine, University of Liverpool, Liverpool L69 3BX, United Kingdom.
authors.forename: Andrew B
authors.initials: AB
authors.lastname: Fielding
mesh_terms: D002458:Cell Fractionation / Q000379:methods*; D002478:Cells, Cultured; D022163:Clathrin-Coated Vesicles