title: [Chitosan/PIK3CA siRNA nanoparticle-mediated PIK3CA gene interference decreases the invasive capacity of gastric cancer cells in vitro].
abstract: OBJECTIVE
  To investigate the effect of PIK3CA/siRNA chitosan nanoparticle on the invasiveness of gastric carcinoma and the potential value of PIK3CA/siRNA chitosan nanoparticle in suppressing the metastasis of gastric carcinoma.
  
  
  METHODS
  Gastric cancer cells were treated with PIK3CA/siRNA nanoparticle (with a diameter of 350 nm), and the efficiency of PIK3CA gene interference was evaluated using Western blotting and real-time PCR. The changes of the invasive capacity of the treated cells was assessed with Transwell assay.
  
  
  RESULTS
  PIK3CA/siRNA chitosan nanoparticle efficiently lowered the expression level of PIK3CA and significantly decreased the invasion of BGC823 cells.
  
  
  CONCLUSIONS
  PIK3CA gene interference mediated by PIK3CA/siRNA chitosan nanoparticle can decrease the invasive capacity of gastric cancer cells in vitro.
authors.affiliation: Affiliated Tumor Hospital of Guangzhou Medical University, Guangzhou 510095, China.E-mail: zxkstar@126.com.
authors.forename: Xinke
authors.initials: X
authors.lastname: Zhou
authors.forename: Lu
authors.initials: L
authors.lastname: He
authors.forename: Min
authors.initials: M
authors.lastname: Liang
authors.forename: Jifang
authors.initials: J
authors.lastname: Liu
mesh_terms: D045744:Cell Line, Tumor; D049109:Cell Proliferation; D048271:Chitosan; D058534:Class I Phosphatidylinositol 3-Kinases; D006801:Humans; D053758:Nanoparticles; D019869:Phosphatidylinositol 3-Kinases / Q000235:genetics*; D034741:RNA, Small Interfering; D060888:Real-Time Polymerase Chain Reaction; D013274:Stomach Neoplasms / Q000473:pathology*
chemical_list: D034741:RNA, Small Interfering; D048271:Chitosan; D019869:Phosphatidylinositol 3-Kinases; D058534:Class I Phosphatidylinositol 3-Kinases; C484760:PIK3CA protein, human