title: A flow-through holed PDMS membrane as a reusable microarray spotter for biomedical assays.
abstract: We propose the exploitation of a holed-designed poly(dimethyl)siloxane (PDMS) membrane as an innovative microarray spotter. The membrane is fabricated by a simple technological approach and can be reused several times. A good level of reproducibility is found upon spotting fluorescent proteins at different concentrations over large areas.
authors.affiliation: Department of Applied Science and Technology - DISAT, Politecnico di Torino, C.so Duca degli Abruzzi 24, 10129 Torino, Italy. andrea.lamberti@polito.it.
authors.forename: A
authors.initials: A
authors.lastname: Lamberti
authors.forename: A
authors.initials: A
authors.lastname: Angelini
authors.forename: S
authors.initials: S
authors.lastname: Ricciardi
authors.forename: F
authors.initials: F
authors.lastname: Frascella
mesh_terms: D004129:Dimethylpolysiloxanes / Q000737:chemistry*; D004867:Equipment Design; D005456:Fluorescent Dyes; D008164:Luminescent Proteins; D008567:Membranes, Artificial; D008751:Methylene Blue; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D015203:Reproducibility of Results
chemical_list: D004129:Dimethylpolysiloxanes; D005456:Fluorescent Dyes; D008164:Luminescent Proteins; D008567:Membranes, Artificial; C013830:baysilon; D008751:Methylene Blue