title: Comparison of test specificities of commercial antigen-based assays and in-house PCR methods for detection of rotavirus in stool specimens.
abstract: Seven commercial rotavirus antigen assays were compared with in-house PCR methods for detecting rotavirus in stool specimens. The assay sensitivities were 80% to 100%, while the specificities were 54.3% for one commercial immunochromatographic (ICT) method and 99.4% to 100% for other assays. Thus, except for one commercial ICT, all the assays were generally reliable for rotavirus detection.
authors.affiliation: Queensland Paediatric Infectious Diseases Laboratory, Queensland Children's Medical Research Institute, The University of Queensland, Brisbane, Queensland, Australia Microbiology Division, Pathology Queensland Central Laboratory, Herston, Queensland, Australia Queensland Children's Medical Research Institute, The University of Queensland, Brisbane, Queensland, Australia.
authors.forename: S
authors.initials: S
authors.lastname: Ye
authors.affiliation: Queensland Children's Medical Research Institute, The University of Queensland, Brisbane, Queensland, Australia Communicable Diseases Unit, Queensland Health, Brisbane, Australia.
authors.forename: S B
authors.initials: SB
authors.lastname: Lambert
authors.affiliation: Queensland Children's Medical Research Institute, The University of Queensland, Brisbane, Queensland, Australia Griffith Health Institute, Griffith University and Gold Coast University Hospital, Gold Coast, Queensland, Australia.
authors.forename: K
authors.initials: K
authors.lastname: Grimwood
authors.affiliation: Enteric Virus Research Group, Murdoch Childrens Research Institute, Parkville, Victoria, Australia.
authors.forename: S
authors.initials: S
authors.lastname: Roczo-Farkas
authors.affiliation: Microbiology Division, Pathology Queensland Central Laboratory, Herston, Queensland, Australia School of Medicine, Griffith University, Gold Coast, Queensland, Australia.
authors.forename: G R
authors.initials: GR
authors.lastname: Nimmo
authors.affiliation: Queensland Paediatric Infectious Diseases Laboratory, Queensland Children's Medical Research Institute, The University of Queensland, Brisbane, Queensland, Australia Queensland Children's Medical Research Institute, The University of Queensland, Brisbane, Queensland, Australia.
authors.forename: T P
authors.initials: TP
authors.lastname: Sloots
authors.affiliation: Enteric Virus Research Group, Murdoch Childrens Research Institute, Parkville, Victoria, Australia Department of Microbiology, La Trobe University, Bundoora, Victoria, Australia.
authors.forename: C D
authors.initials: CD
authors.lastname: Kirkwood
authors.affiliation: Queensland Paediatric Infectious Diseases Laboratory, Queensland Children's Medical Research Institute, The University of Queensland, Brisbane, Queensland, Australia Queensland Children's Medical Research Institute, The University of Queensland, Brisbane, Queensland, Australia d.whiley@uq.edu.au.
authors.forename: D M
authors.initials: DM
authors.lastname: Whiley
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002648:Child; D002675:Child, Preschool; D002846:Chromatography, Affinity / Q000379:methods*; D005243:Feces / Q000821:virology*; D005260:Female; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction / Q000379:methods*; D015203:Reproducibility of Results; D012401:Rotavirus / Q000235:genetics* / Q000302:isolation & purification*; D012400:Rotavirus Infections / Q000175:diagnosis* / Q000821:virology*; D012680:Sensitivity and Specificity; D055815:Young Adult