title: Morphological transitions under oxidative stress in response to metabolite formation in Aspergillus niger.
abstract: Oxidative stress is associated with metabolite formation in fungi. In contrast to an Aspergillus niger wild-type strain, a sclerotia-formation regulator ansclR deletion strain demonstrated increased susceptibility to oxidative stress and reduced transcription of the catalase gene, catB, while an ansclR overexpression strain showed enhanced resistance to oxidative stress and increased expression of catB. In addition, ansclR complementation strain expressed a wild-type level of catB. The ansclR overexpression strain also produced the same metabolites as the wild type strain treated with H2O2. Furthermore, LC-MS, NMR, and IR analyses showed that the main metabolite was a steroid analog. Our study adds new clues to oxidative stress-related factors and metabolite formation in A. niger.
authors.affiliation: Department of Bioengineering, School of Bioscience and Bioengineering, South China University of Technology, Guangzhou, People's Republic of China.
authors.forename: Yangyong
authors.initials: Y
authors.lastname: Lv
authors.forename: Feng
authors.initials: F
authors.lastname: Zhou
authors.forename: Bin
authors.initials: B
authors.lastname: Wang
authors.forename: Li
authors.initials: L
authors.lastname: Pan
mesh_terms: D001234:Aspergillus niger / Q000187:drug effects* / Q000254:growth & development* / Q000378:metabolism / Q000502:physiology; D002374:Catalase / Q000235:genetics / Q000378:metabolism; D002853:Chromatography, Liquid; D017353:Gene Deletion; D015870:Gene Expression; D005816:Genetic Complementation Test; D006861:Hydrogen Peroxide / Q000633:toxicity; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D018384:Oxidative Stress; D013055:Spectrophotometry, Infrared; D013256:Steroids / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism
chemical_list: D013256:Steroids; D014157:Transcription Factors; D006861:Hydrogen Peroxide; D002374:Catalase