title: A piglet with surgically induced exocrine pancreatic insufficiency as an animal model of newborns to study fat digestion.
abstract: The maldigestion and malabsorption of fat in infants fed milk formula results due to the minimal production of pancreatic lipase. Thus, to investigate lipid digestion and absorption and mimic the situation in newborns, a young porcine exocrine pancreatic insufficient (EPI) model was adapted and validated in the present study. A total of thirteen EPI pigs, aged 8 weeks old, were randomised into three groups and fed either a milk-based formula or a milk-based formula supplemented with either bacterial or fungal lipase. Digestion and absorption of fat was directly correlated with the addition of lipases as demonstrated by a 30% increase in the coefficient of fat absorption. In comparison to the control group, a 40 and 25% reduction in total fat content and 26 and 45% reduction in n-3 and n-6 fatty acid (FA) content in the stool was observed for lipases 1 and 2, respectively. Improved fat absorption was reflected in the blood levels of lipid parameters. During the experiment, only a very slight gain in body weight was observed in EPI piglets, which can be explained by the absence of pancreatic protease and amylase in the gastrointestinal tract. This is similar to newborn babies that have reduced physiological function of exocrine pancreas. In conclusion, we postulate that the EPI pig model fed with infant formula mimics the growth and lipid digestion and absorption in human neonates and can be used to elucidate further importance of fat and FA in the development and growth of newborns, as well as for testing novel formula compositions.
authors.affiliation: Department of Biology,Lund University,Lund,Sweden.
authors.forename: Kateryna
authors.initials: K
authors.lastname: Goncharova
authors.affiliation: Department of Biology,Lund University,Lund,Sweden.
authors.forename: Stefan G
authors.initials: SG
authors.lastname: Pierzynowski
authors.affiliation: Alcresta,Newtön,MA,USA.
authors.forename: Danica
authors.initials: D
authors.lastname: Grujic
authors.affiliation: Division of Biochemical Pharmacology, Institute of Pharmacology and Biochemistry,Grodno,Republic of Belarus.
authors.forename: Siarhei
authors.initials: S
authors.lastname: Kirko
authors.affiliation: Department of Biology,Lund University,Lund,Sweden.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Szwiec
authors.affiliation: Department of Biology,Lund University,Lund,Sweden.
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Cytology,Bogomoletz Institute of Physiology,Kyiv,Ukraine.
authors.forename: Tetiana
authors.initials: T
authors.lastname: Kovalenko
authors.affiliation: Department of Cytology,Bogomoletz Institute of Physiology,Kyiv,Ukraine.
authors.forename: Iryna
authors.initials: I
authors.lastname: Osadchenko
authors.affiliation: Department of Biophysics and Biochemistry,Oles Honchar Dnepropetrovsk National University,Dnepropetrovsk,Ukraine.
authors.forename: Galyna
authors.initials: G
authors.lastname: Ushakova
authors.affiliation: Department of Biochemistry,Vasyl Stefanyk Prycarpathian National University,Ivano-Frankivsk,Ukraine.
authors.forename: Halyna
authors.initials: H
authors.lastname: Shmigel
authors.affiliation: SGPlus,Malmö,Sweden.
authors.forename: Olexandr
authors.initials: O
authors.lastname: Fedkiv
authors.affiliation: Department of Biology,Lund University,Lund,Sweden.
authors.forename: Blanka
authors.initials: B
authors.lastname: Majda
authors.affiliation: SGPlus,Malmö,Sweden.
authors.forename: Olena
authors.initials: O
authors.lastname: Prykhodko
mesh_terms: D000818:Animals; D001835:Body Weight; D004042:Dietary Fats, Unsaturated / Q000378:metabolism*; D004063:Digestion; D004195:Disease Models, Animal; D010188:Exocrine Pancreatic Insufficiency / Q000209:etiology / Q000378:metabolism*; D015525:Fatty Acids, Omega-3 / Q000378:metabolism; D043371:Fatty Acids, Omega-6 / Q000378:metabolism; D005243:Feces; D041981:Gastrointestinal Tract / Q000378:metabolism; D006128:Growth; D006801:Humans; D041943:Infant Formula; D007231:Infant, Newborn; D007408:Intestinal Absorption; D008026:Ligation; D008049:Lipase / Q000172:deficiency* / Q000494:pharmacology; D050356:Lipid Metabolism; D008297:Male; D008892:Milk; D046790:Pancreas, Exocrine; D010183:Pancreatic Ducts / Q000601:surgery; D011897:Random Allocation; D013552:Swine
chemical_list: D004042:Dietary Fats, Unsaturated; D015525:Fatty Acids, Omega-3; D043371:Fatty Acids, Omega-6; D008049:Lipase