title: Extracellular matrix-immobilized nanotopographical substrates for enhanced myogenic differentiation.
abstract: Extracellular matrix (ECM) components such as fibronectin (FN) and laminin (LMN) play prominent roles in controlling cellular behaviors. Many attempts have been made to explore cellular behaviors on combinatorial ECM arrays in high-throughput systems. However these studies were limited to physical adsorption of ECM, which does not guarantee a lasting effect of ECM in vitro. Here, we demonstrate ECM immobilization on polyurethane acrylate (PUA) substrate fabricated in 24 well-plate platforms to effectively differentiate C2C12. Our study demonstrate that co-immobilization of FN and LMN was found to enhance myogenic differentiation of C2C12 cells compared to single immobilization of either FN or LMN alone. Furthermore, utilizing nano-imprint lithography technique, 300 nm and 5 µm line-patterned substrates were fabricated on 24-well plates. FN and LMN co-immobilized substrates with line-patterns additionally provided the directionality for mimicking musculoskeletal structure and enhanced the myogenic differentiation.
authors.affiliation: School of Chemical and Biological Engineering, Seoul National University, Seoul, 151-744, Republic of Korea.
authors.forename: Eunjee A
authors.initials: EA
authors.lastname: Lee
authors.affiliation: School of Chemical and Biological Engineering, Seoul National University, Seoul, 151-744, Republic of Korea.
authors.forename: Giyoung
authors.initials: G
authors.lastname: Jung
authors.affiliation: Department of Chemical and Biomolecular Engineering and KI for the NanoCentury, Korea Advanced Institute of Science and Technology, Daejeon, 305-701, Republic of Korea.
authors.forename: Sung Gap
authors.initials: SG
authors.lastname: Im
authors.affiliation: School of Chemical and Biological Engineering, Seoul National University, Seoul, 151-744, Republic of Korea.|The BioMAX Institute of Seoul National University, Seoul National University, Seoul, Republic of Korea.
authors.forename: Nathaniel S
authors.initials: NS
authors.lastname: Hwang
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D002460:Cell Line; D005109:Extracellular Matrix / Q000737:chemistry*; D051379:Mice; D024510:Muscle Development; D018485:Muscle Fibers, Skeletal / Q000166:cytology / Q000378:metabolism*; D049329:Nanostructures / Q000737:chemistry*
keywords: extracellular matrix; myogenic differentiation; nanotopography; surface modification