title: A 90-day subchronic feeding study of genetically modified rice expressing Cry1Ab protein in Sprague-Dawley rats.
abstract: Bacillus thuringiensis (Bt) transgenic rice line (mfb-MH86) expressing a synthetic cry1Ab gene can be protected against feeding damage from Lepidopteran insects, including Sesamia inferens, Chilo suppressalis, Tryporyza incertulas and Cnaphalocrocis medinalis. Rice flour from mfb-MH86 and its near-isogenic control MH86 was separately formulated into rodent diets at concentrations of 17.5, 35 and 70 % (w/w) for a 90-day feeding test with rats, and all of the diets were nutritionally balanced. In this study, the responses of rats fed diets containing mfb-MH86 were compared to those of rats fed flour from MH86. Overall health, body weight and food consumption were comparable between groups fed diets containing mfb-MH86 and MH86. Blood samples were collected prior to sacrifice and a few significant differences (p < 0.05) were observed in haematological and biochemical parameters between rats fed genetically modified (GM) and non-GM diets. However, the values of these parameters were within the normal ranges of values for rats of this age and sex, thus not considered treatment related. In addition, upon sacrifice a large number of organs were weighed, macroscopic and histopathological examinations were performed with only minor changes to report. In conclusion, these results demonstrated that no toxic effect was observed in the conditions of the experiment, based on the different parameters assessed. GM rice mfb-MH86 is as safe and nutritious as non-GM rice.
authors.affiliation: College of Food Science and Nutritional Engineering, China Agricultural University, No. 17 Tsinghua Donglu, Beijing, 100083, China.
authors.forename: Huan
authors.initials: H
authors.lastname: Song
authors.forename: Xiaoyun
authors.initials: X
authors.lastname: He
authors.forename: Shiying
authors.initials: S
authors.lastname: Zou
authors.forename: Teng
authors.initials: T
authors.lastname: Zhang
authors.forename: Yunbo
authors.initials: Y
authors.lastname: Luo
authors.forename: Kunlun
authors.initials: K
authors.lastname: Huang
authors.forename: Zhen
authors.initials: Z
authors.lastname: Zhu
authors.forename: Wentao
authors.initials: W
authors.lastname: Xu
mesh_terms: D000818:Animals; D001413:Bacillus thuringiensis / Q000235:genetics; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000235:genetics*; D004033:Diet Fads; D004731:Endotoxins / Q000235:genetics*; D005433:Flour / Q000009:adverse effects*; D006460:Hemolysin Proteins / Q000235:genetics*; D012275:Oryza / Q000009:adverse effects / Q000235:genetics*; D030821:Plants, Genetically Modified / Q000009:adverse effects / Q000235:genetics*; D051381:Rats
chemical_list: D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D004731:Endotoxins; D006460:Hemolysin Proteins; C030956:insecticidal crystal protein, Bacillus Thuringiensis