title: A novel antibacterial peptide active against peach crown gall (Agrobacterium tumefaciens) isolated from cyanide-tolerant actinomycetes G19.
abstract: An antimicrobial peptide was extracted from the antagonistic actinomycetes G19. It was designated as G19-F. By using MALDI-TOF mass spectrometry, the molecular weight of G19-F was determined. The primary structure of the antimicrobial peptide was determined using N-terminal sequencing and mass spectrometry. Results showed that the peptide had eleven amino acids, with the sequence D-V-C-D-G-G-D-G-D-E-D, and a calculated molecular mass of 1,096 Da. G19-F showed antimicrobial activity against peach crown gall caused by Agrobacterium tumefaciens. The antimicrobial peptide maintained its activity after being heated to 100 °C and exhibited stability from pH 4 to 10. Its activity has also remained after ultraviolet irradiation. The mechanism by which G19-F inhibits A. tumefaciens was to increase permeability of the cell membrane and destroy the cell wall structure. Furthermore, as a novel peptide, it has a potential for cure A. tumefaciens infection.
authors.affiliation: Department of Plant Science and Technology, Beijing University of Agriculture, No.7 Beinong road, Huilongguan, Changping, Beijing, 102206, China.
authors.forename: Shufang
authors.initials: S
authors.lastname: Wang
authors.forename: Jinglin
authors.initials: J
authors.lastname: Ji
authors.forename: Huanpu
authors.initials: H
authors.lastname: Ma
authors.forename: Zhimin
authors.initials: Z
authors.lastname: Liu
mesh_terms: D039903:Actinobacteria / Q000737:chemistry*; D016960:Agrobacterium tumefaciens / Q000187:drug effects* / Q000254:growth & development; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D002462:Cell Membrane / Q000187:drug effects / Q000502:physiology; D006863:Hydrogen-Ion Concentration; D013058:Mass Spectrometry; D008970:Molecular Weight; D010455:Peptides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D010539:Permeability / Q000187:drug effects; D010941:Plant Tumors / Q000382:microbiology*; D027861:Prunus / Q000382:microbiology; D020539:Sequence Analysis, Protein; D013696:Temperature
chemical_list: D000900:Anti-Bacterial Agents; D010455:Peptides