title: CSF proteomics of secondary phase spinal cord injury in human subjects: perturbed molecular pathways post injury.
abstract: Recovery of sensory and motor functions following traumatic spinal cord injury (SCI) is dependent on injury severity. Here we identified 49 proteins from cerebrospinal fluid (CSF) of SCI patients, eight of which were differentially abundant among two severity groups of SCI. It was observed that the abundance profiles of these proteins change over a time period of days to months post SCI. Statistical analysis revealed that these proteins take part in several molecular pathways including DNA repair, protein phosphorylation, tRNA transcription, iron transport, mRNA metabolism, immune response and lipid and ATP catabolism. These pathways reflect a set of mechanisms that the system may adopt to cope up with the assault depending on the injury severity, thus leading to observed physiological responses. Apart from putting forward a picture of the molecular scenario at the injury site in a human study, this finding further delineates consequent pathways and molecules that may be altered by external intervention to restrict neural degeneration.
authors.affiliation: Biophysics and Structural Genomics Division, Saha Institute of Nuclear Physics, Kolkata, West Bengal, India.
authors.forename: Mohor Biplab
authors.initials: MB
authors.lastname: Sengupta
authors.affiliation: Condensed Matter Physics Division, Saha Institute of Nuclear Physics, Kolkata, West Bengal, India.
authors.forename: Mahashweta
authors.initials: M
authors.lastname: Basu
authors.affiliation: Department of Physical Medicine & Rehabilitation, Nil Ratan Sircar Medical College & Hospital, Kolkata, West Bengal, India.
authors.forename: Sourav
authors.initials: S
authors.lastname: Iswarari
authors.affiliation: Department of Orthopaedic Surgery, Nil Ratan Sircar Medical College & Hospital, Kolkata, West Bengal, India.
authors.forename: Kiran Kumar
authors.initials: KK
authors.lastname: Mukhopadhyay
authors.affiliation: Department of Orthopaedic Surgery, Nil Ratan Sircar Medical College & Hospital, Kolkata, West Bengal, India.
authors.forename: Krishna Pada
authors.initials: KP
authors.lastname: Sardar
authors.affiliation: Department of Orthopaedic Surgery, Nil Ratan Sircar Medical College & Hospital, Kolkata, West Bengal, India.
authors.forename: Biplab
authors.initials: B
authors.lastname: Acharyya
authors.affiliation: Condensed Matter Physics Division, Saha Institute of Nuclear Physics, Kolkata, West Bengal, India.
authors.forename: Pradeep K
authors.initials: PK
authors.lastname: Mohanty
authors.affiliation: Biophysics and Structural Genomics Division, Saha Institute of Nuclear Physics, Kolkata, West Bengal, India.
authors.forename: Debashis
authors.initials: D
authors.lastname: Mukhopadhyay
mesh_terms: D000328:Adult; D004586:Electrophoresis; D006429:Hemiplegia / Q000134:cerebrospinal fluid; D006801:Humans; D008297:Male; D008875:Middle Aged; D025941:Protein Interaction Mapping; D020543:Proteome / Q000378:metabolism*; D020127:Recovery of Function / Q000502:physiology; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013116:Spinal Cord / Q000378:metabolism; D013119:Spinal Cord Injuries / Q000134:cerebrospinal fluid* / Q000473:pathology*; D055815:Young Adult
chemical_list: D020543:Proteome