title: Vitamin D Status Affects Serum Metabolomic Profiles in Pregnant Adolescents.
abstract: Vitamin D is linked to a number of adverse pregnancy outcomes through largely unknown mechanisms. This study was conducted to examine the role of vitamin D status in metabolomic profiles in a group of 30 pregnant, African American adolescents (17.1 ± 1.1 years) at midgestation (26.8 ± 2.8 weeks), in 15 adolescents with 25-hydroxy vitamin D (25(OH)D) ≥20 ng/mL, and in 15 teens with 25(OH)D <20 ng/mL. Serum metabolomic profiles were examined using gas chromatography-mass spectrometry and liquid chromatography-tandem mass spectrometry. A novel hierarchical mixture model was used to evaluate differences in metabolite profiles between low and high groups. A total of 326 compounds were identified and included in subsequent statistical analyses. Eleven metabolites had significantly different means between the 2 vitamin D groups, after correcting for multiple hypothesis testing: pyridoxate, bilirubin, xylose, and cholate were higher, and leukotrienes, 1,2-propanediol, azelate, undecanedioate, sebacate, inflammation associated complement component 3 peptide (HWESASXX), and piperine were lower in serum from adolescents with 25(OH)D ≥20 ng/mL. Lower maternal vitamin D status at midgestation impacted serum metabolic profiles in pregnant adolescents.
authors.affiliation: Division of Nutritional Sciences, Cornell University, Ithaca, NY, USA.
authors.forename: Julia L
authors.initials: JL
authors.lastname: Finkelstein
authors.affiliation: Department of Obstetrics and Gynecology, University of Rochester, Rochester, NY, USA.
authors.forename: Eva K
authors.initials: EK
authors.lastname: Pressman
authors.affiliation: Department of Obstetrics and Gynecology, University of Rochester, Rochester, NY, USA.
authors.forename: Elizabeth M
authors.initials: EM
authors.lastname: Cooper
authors.affiliation: Division of Nutritional Sciences, Cornell University, Ithaca, NY, USA.
authors.forename: Tera R
authors.initials: TR
authors.lastname: Kent
authors.affiliation: Department of Statistics, University of Connecticut, Storrs, CT, USA.
authors.forename: Haim Y
authors.initials: HY
authors.lastname: Bar
authors.affiliation: Division of Nutritional Sciences, Cornell University, Ithaca, NY, USA koo4@cornell.edu.
authors.forename: Kimberly O
authors.initials: KO
authors.lastname: O'Brien
mesh_terms: D000293:Adolescent; D001741:Black or African American; D000367:Age Factors; D015415:Biomarkers / Q000097:blood; D002853:Chromatography, Liquid; D016000:Cluster Analysis; D004734:Energy Metabolism; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry; D005865:Gestational Age; D006801:Humans; D007422:Intestines / Q000382:microbiology; D055432:Metabolomics / Q000379:methods; D064307:Microbiota; D009518:New York / Q000453:epidemiology; D011247:Pregnancy; D011253:Pregnancy in Adolescence / Q000097:blood* / Q000208:ethnology; D015995:Prevalence; D053719:Tandem Mass Spectrometry; D014807:Vitamin D / Q000031:analogs & derivatives* / Q000097:blood; D014808:Vitamin D Deficiency / Q000097:blood* / Q000175:diagnosis / Q000208:ethnology
chemical_list: D015415:Biomarkers; D014807:Vitamin D; C104450:25-hydroxyvitamin D
keywords: adolescent; biomarker; metabolomics; pregnancy; vitamin D