title: Endothelial cells use dynamic actin to facilitate lymphocyte transendothelial migration and maintain the monolayer barrier.
abstract: The vascular endothelium is a highly dynamic structure, and the integrity of its barrier function is tightly regulated. Normally impenetrable to cells, the endothelium actively assists lymphocytes to exit the bloodstream during inflammation. The actin cytoskeleton of the endothelial cell (EC) is known to facilitate transmigration, but the cellular and molecular mechanisms are not well understood. Here we report that actin assembly in the EC, induced by Arp2/3 complex under control of WAVE2, is important for several steps in the process of transmigration. To begin transmigration, ECs deploy actin-based membrane protrusions that create a cup-shaped docking structure for the lymphocyte. We found that docking structure formation involves the localization and activation of Arp2/3 complex by WAVE2. The next step in transmigration is creation of a migratory pore, and we found that endothelial WAVE2 is needed for lymphocytes to follow a transcellular route through an EC. Later, ECs use actin-based protrusions to close the gap behind the lymphocyte, which we discovered is also driven by WAVE2. Finally, we found that ECs in resting endothelial monolayers use lamellipodial protrusions dependent on WAVE2 to form and maintain contacts and junctions between cells.
authors.affiliation: Department of Cell Biology and Physiology, Washington University, St. Louis, MO 63110.
authors.forename: Olivia L
authors.initials: OL
authors.lastname: Mooren
authors.affiliation: Department of Cell Biology and Physiology, Washington University, St. Louis, MO 63110.
authors.forename: Jinmei
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Cell Biology and Physiology, Washington University, St. Louis, MO 63110.
authors.forename: Julie
authors.initials: J
authors.lastname: Nawas
authors.affiliation: Department of Cell Biology and Physiology, Washington University, St. Louis, MO 63110 jcooper@wustl.edu.
authors.forename: John A
authors.initials: JA
authors.lastname: Cooper
mesh_terms: D008841:Actin Cytoskeleton / Q000378:metabolism / Q000648:ultrastructure; D051376:Actin-Related Protein 2-3 Complex / Q000502:physiology; D000199:Actins / Q000378:metabolism; D002199:Capillary Permeability; D002478:Cells, Cultured; D017097:Electric Impedance; D042783:Endothelial Cells / Q000502:physiology*; D004730:Endothelium, Vascular / Q000166:cytology; D006801:Humans; D008214:Lymphocytes / Q000502:physiology*; D021381:Protein Transport; D057705:Transendothelial and Transepithelial Migration; D051300:Wiskott-Aldrich Syndrome Protein Family / Q000502:physiology
chemical_list: D051376:Actin-Related Protein 2-3 Complex; D000199:Actins; C120152:WASF2 protein, human; D051300:Wiskott-Aldrich Syndrome Protein Family