title: Advanced bile acid-based multi-compartmental microencapsulated pancreatic β-cells integrating a polyelectrolyte-bile acid formulation, for diabetes treatment.
abstract: This study utilized the Seahorse Analyzer to examine the effect of the bile acid ursodeoxycholic acid (UDCA), on the morphology, swelling, stability, and size of novel microencapsulated β-cells, in real-time. UDCA was conjugated with fluorescent compounds, and its partitioning within the microcapsules was examined using confocal microscopy. UDCA produced microcapsules with good morphology, better mechanical strength (p < 0.01), and reduced swelling properties (p < 0.01), but lower cell viability (p < 0.05) and cell count per microcapsule (p < 0.01). UDCA reduced the cells' biochemical activities, mitochondrial respiration, and energy production, post-microencapsulation. This is the first time biological functions of microencapsulated β-cells have been analyzed in real-time.
authors.affiliation: a Biotechnology and Drug Development Research Laboratory, School of Pharmacy, Curtin Health Innovation Research Institute, Biosciences Research Precinct, Curtin University , Perth , Western Australia , Australia.
authors.forename: Armin
authors.initials: A
authors.lastname: Mooranian
authors.affiliation: a Biotechnology and Drug Development Research Laboratory, School of Pharmacy, Curtin Health Innovation Research Institute, Biosciences Research Precinct, Curtin University , Perth , Western Australia , Australia.
authors.forename: Rebecca
authors.initials: R
authors.lastname: Negrulj
authors.affiliation: b Faculty of Science and Engineering, Department of Imaging and Applied Physics , Curtin University , Perth , Western Australia , Australia.
authors.forename: Nigel
authors.initials: N
authors.lastname: Chen-Tan
authors.affiliation: c Faculty of Medicine, Department of Neuroscience , University of Montreal , Montreal , Quebec , Canada.
authors.forename: Marc
authors.initials: M
authors.lastname: Fakhoury
authors.affiliation: d Curtin Health Innovation Research Institute, Biosciences Research Precinct, School of Biomedical Science, Curtin University , Perth , Western Australia , Australia.
authors.forename: Frank
authors.initials: F
authors.lastname: Arfuso
authors.affiliation: e Faculty of Science and Engineering, Department of Chemistry , Curtin University , Perth , Western Australia , Australia.
authors.forename: Franca
authors.initials: F
authors.lastname: Jones
authors.affiliation: a Biotechnology and Drug Development Research Laboratory, School of Pharmacy, Curtin Health Innovation Research Institute, Biosciences Research Precinct, Curtin University , Perth , Western Australia , Australia.
authors.forename: Hani
authors.initials: H
authors.lastname: Al-Salami
mesh_terms: D002214:Capsules; D002460:Cell Line; D019069:Cell Respiration / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002626:Chemistry, Pharmaceutical; D003920:Diabetes Mellitus / Q000188:drug therapy* / Q000473:pathology*; D004355:Drug Stability; D005456:Fluorescent Dyes / Q000737:chemistry; D050417:Insulin-Secreting Cells / Q000187:drug effects* / Q000473:pathology; D055595:Mechanical Phenomena; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism; D014580:Ursodeoxycholic Acid / Q000737:chemistry* / Q000494:pharmacology* / Q000627:therapeutic use
chemical_list: D002214:Capsules; D005456:Fluorescent Dyes; D014580:Ursodeoxycholic Acid
keywords: Seahorse analyzer; bile acid; microencapsulation; pancreatic beta cells