title: The Rts1 regulatory subunit of PP2A phosphatase controls expression of the HO endonuclease via localization of the Ace2 transcription factor.
abstract: The RTS1 gene encodes a subunit of the PP2A phosphatase that regulates cell cycle progression. Ace2 and Swi5 are cell cycle-regulated transcription factors, and we recently showed that phosphorylation of Ace2 and Swi5 is altered in an rts1 mutant. Here we examine expression of Ace2 and Swi5 target genes and find that an rts1 mutation markedly reduces expression of the HO gene. The decreased HO expression in an rts1 mutant is significantly restored by an additional ace2 mutation, a surprising result because HO is normally activated by Swi5 but not by Ace2. Ace2 normally accumulates only in daughter cells, and only activates transcription in daughters. However, in an rts1 mutant, Ace2 is present in both mother and daughter cells. One of the genes activated by Ace2 is ASH1, a protein that normally accumulates mostly in daughter cells; Ash1 is a transcriptional repressor, and it blocks HO expression in daughters. We show that in the rts1 mutant, Ace2 accumulation in mother cells results in Ash1 expression in mothers, and the Ash1 can now repress HO expression in mothers.
authors.affiliation: From the Department of Pathology, University of Utah Health Sciences Center, Salt Lake City, Utah 84112.
authors.forename: Emily J
authors.initials: EJ
authors.lastname: Parnell
authors.affiliation: From the Department of Pathology, University of Utah Health Sciences Center, Salt Lake City, Utah 84112.
authors.forename: Yaxin
authors.initials: Y
authors.lastname: Yu
authors.affiliation: the Department of Molecular, Cell and Developmental Biology, University of California, Santa Cruz, California 95064, and.
authors.forename: Rafael
authors.initials: R
authors.lastname: Lucena
authors.affiliation: the Center for Eukaryotic Gene Regulation, Department of Biochemistry and Molecular Biology, and.
authors.forename: Youngdae
authors.initials: Y
authors.lastname: Yoon
authors.affiliation: the Center for Eukaryotic Gene Regulation, Department of Biochemistry and Molecular Biology, and Department of Physics, The Pennsylvania State University, University Park, Pennsylvania 16802.
authors.forename: Lu
authors.initials: L
authors.lastname: Bai
authors.affiliation: the Department of Molecular, Cell and Developmental Biology, University of California, Santa Cruz, California 95064, and.
authors.forename: Douglas R
authors.initials: DR
authors.lastname: Kellogg
authors.affiliation: From the Department of Pathology, University of Utah Health Sciences Center, Salt Lake City, Utah 84112, david.stillman@path.utah.edu.
authors.forename: David J
authors.initials: DJ
authors.lastname: Stillman
mesh_terms: D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D015252:Deoxyribonucleases, Type II Site-Specific / Q000235:genetics* / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic; D015966:Gene Expression Regulation, Fungal; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D009154:Mutation; D054648:Protein Phosphatase 2 / Q000235:genetics* / Q000378:metabolism; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics* / Q000378:metabolism; D059008:Time-Lapse Imaging; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism
chemical_list: C066778:ACE2 protein, S cerevisiae; C098733:ASH1 protein, S cerevisiae; D018797:Cell Cycle Proteins; D004268:DNA-Binding Proteins; D012097:Repressor Proteins; C080218:SWI5 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; D049452:Green Fluorescent Proteins; C530845:HO protein, S cerevisiae; D015252:Deoxyribonucleases, Type II Site-Specific; D054648:Protein Phosphatase 2; C584366:Rts1 protein, S cerevisiae
keywords: Cell Cycle; Gene Regulation; Nuclear Translocation; Phosphatase; Yeast Transcription