title: MicroRNA-146a rs2910164 polymorphism and the risk of diffuse large B cell lymphoma in the Chinese Han population.
abstract: The aim of this study was to determine the association of the microRNA-146a (miR-146a) polymorphism with the risk of diffuse large B cell lymphoma (DLBCL). The genotyping of miR-146a rs2910164 polymorphism was detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). The results showed that CC genotype and C alleles distribution in the DLBCL patient was significantly higher than that of the controls (p = 0.01 and p = 0.01, respectively). No significant differences were found between the two subgroups when stratified by clinical characteristics including sexual, age at admission, performance status, pathological type, Ann Arbor stage, LDH and β2-MG value. The miR-146a expression was detected by the Taqman real-time PCR. The result showed that the miR-146a expression was notably upregulated in DLBCL patients when compared with controls (p = 0.02). In addition, the miR-146a expression of CC genotypes subgroup was drastically downregulated than that of GC/GG genotype subgroup in DLBCL patients (p = 0.0003), suggesting that this polymorphism can functionally affect the expression of miR-146a. In conclusion, it was shown that the miR-146a rs2910164 polymorphism is associated with the risk of DLBCL in the Chinese Han population.
authors.affiliation: Department of Hematology, The First Affiliated Hospital of Zhejiang Chinese Medicine University, No.54 You-dian Road, Hangzhou, 310006, Zhejiang, People's Republic of China.
authors.forename: Haifeng
authors.initials: H
authors.lastname: Zhuang
authors.forename: Jianping
authors.initials: J
authors.lastname: Shen
authors.forename: Zhiyin
authors.initials: Z
authors.lastname: Zheng
authors.forename: Xiusu
authors.initials: X
authors.lastname: Luo
authors.forename: Ruilan
authors.initials: R
authors.lastname: Gao
authors.forename: Xiaofen
authors.initials: X
authors.lastname: Zhuang
mesh_terms: D000368:Aged; D044466:Asian People / Q000235:genetics; D016022:Case-Control Studies; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D006801:Humans; D016403:Lymphoma, Large B-Cell, Diffuse / Q000235:genetics*; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide
chemical_list: C542542:MIRN146 microRNA, human; D035683:MicroRNAs