title: The influence of particle size and static magnetic fields on the uptake of magnetic nanoparticles into three dimensional cell-seeded collagen gel cultures.
abstract: Over recent decades there has been and continues to be major advances in the imaging, diagnosis and potential treatment of medical conditions, by the use of magnetic nanoparticles. However, to date the majority of cell delivery studies employ a traditional 2D monolayer culture. This article aims to determine the ability of various sized magnetic nanoparticles to penetrate and travel through a cell seeded collagen gel model, in the presence or absence of a magnetic field. Three different sized (100, 200, and 500 nm) nanoparticles were employed in the study. The results showed cell viability was unaffected by the presence of nanoparticles over a 24-h test period. The initial uptake of the 100 nm nanoparticle into the collagen gel structure was superior compared to the larger sized nanoparticles under the influence of a magnetic field and incubated for 24 h. Interestingly, it was the 200 nm nanoparticles, which proved to penetrate the gel furthest, under the influence of a magnetic field, during the initial culture stage after 1-h incubation.
authors.affiliation: Centre for Cell Engineering, University of Glasgow, Glasgow, UK, G12 8QQ.
authors.forename: Emily E L
authors.initials: EE
authors.lastname: Lewis
authors.affiliation: Centre for Cell Engineering, University of Glasgow, Glasgow, UK, G12 8QQ.
authors.forename: Hannah W
authors.initials: HW
authors.lastname: Child
authors.affiliation: School of Science, University of the West of Scotland, Paisley, UK, PA1 2BE.
authors.forename: Andrew
authors.initials: A
authors.lastname: Hursthouse
authors.affiliation: School of Science, University of the West of Scotland, Paisley, UK, PA1 2BE.
authors.forename: David
authors.initials: D
authors.lastname: Stirling
authors.affiliation: Centre for Cell Engineering, University of Glasgow, Glasgow, UK, G12 8QQ.
authors.forename: Mark
authors.initials: M
authors.lastname: McCully
authors.affiliation: Centre for Cell Engineering, University of Glasgow, Glasgow, UK, G12 8QQ.
authors.forename: David
authors.initials: D
authors.lastname: Paterson
authors.affiliation: Electron Microscopy Unit, University of Glasgow, Glasgow, UK, G12 8QQ.
authors.forename: Margaret
authors.initials: M
authors.lastname: Mullin
authors.affiliation: Centre for Cell Engineering, University of Glasgow, Glasgow, UK, G12 8QQ.
authors.forename: Catherine C
authors.initials: CC
authors.lastname: Berry
mesh_terms: D002461:Cell Line, Transformed; D017690:Cell Transplantation / Q000379:methods; D003094:Collagen / Q000737:chemistry*; D005782:Gels / Q000737:chemistry; D006801:Humans; D060526:Magnetic Fields; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D010316:Particle Size
chemical_list: D005782:Gels; D058185:Magnetite Nanoparticles; D003094:Collagen
keywords: 3D culture; collagen gels; magnetic nanoparticles; nanotechnology