title: Laser-assisted generation of human induced pluripotent stem cells.
abstract: This unit describes a procedure for generating human induced pluripotent stem cells (hiPSCs) using the Laser-Enabled Analysis and Processing (LEAP®) system, which combines high-throughput cell imaging with laser-mediated cell manipulation. Use of this system should not only improve the quality and uniformity of hiPSCs produced, but ultimately enable a more rapid, efficient, high-throughput, and automated production process.
authors.affiliation: Stem Cell Research Center and Core Facility, Sanford-Burnham Medical Research Institute, La Jolla, California.
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.forename: Dongmei
authors.initials: D
authors.lastname: Wu
authors.forename: Donglin
authors.initials: D
authors.lastname: Lao
authors.forename: Cory
authors.initials: C
authors.lastname: Peterson
authors.forename: Kristi A
authors.initials: KA
authors.lastname: Hohenstein Elliott
authors.forename: Evan Y
authors.initials: EY
authors.lastname: Snyder
mesh_terms: D000067470:Cellular Reprogramming Techniques / Q000379:methods*; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology* / Q000378:metabolism*; D007834:Lasers
keywords: automated; high-throughput; iPSC generation; laser-assisted