title: Nanoparticle-assisted laser desorption/ionization for metabolite imaging.
abstract: Matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI-IMS) has enabled the spatial analysis of various molecules, including peptides, nucleic acids, lipids, and drug molecules. To expand the capabilities of MALDI-IMS, we have established an imaging technique using metal nanoparticles (NPs) to visualize metabolites, termed nanoparticle-assisted laser desorption/ionization imaging mass spectrometry (nano-PALDI-IMS). By utilizing Ag-, Fe-, Au-, and TiO2-derived NPs, we have succeeded in visualizing various metabolites, including fatty acid and glycosphingolipids, with higher sensitivity and spatial resolution than conventional techniques. Herein, we describe the practical experimental procedures and methods associated with nano-PALDI-IMS for the visualization of these molecules.
authors.affiliation: Department of Cell Biology and Anatomy, Hamamatsu University School of Medicine, 1-20-1, Handayama, Higashi-ku, Hamamatsu-shi, Shizuoka, Japan.
authors.forename: Michihiko
authors.initials: M
authors.lastname: Waki
authors.forename: Eiji
authors.initials: E
authors.lastname: Sugiyama
authors.forename: Takeshi
authors.initials: T
authors.lastname: Kondo
authors.forename: Keigo
authors.initials: K
authors.lastname: Sano
authors.forename: Mitsutoshi
authors.initials: M
authors.lastname: Setou
mesh_terms: D000818:Animals; D001921:Brain / Q000166:cytology / Q000378:metabolism; D006046:Gold / Q000737:chemistry; D007501:Iron / Q000737:chemistry; D051379:Mice; D008810:Mice, Inbred C57BL; D057054:Molecular Imaging / Q000379:methods*; D053758:Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D012834:Silver / Q000737:chemistry; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D014025:Titanium / Q000737:chemistry
chemical_list: C009495:titanium dioxide; D012834:Silver; D006046:Gold; D014025:Titanium; D007501:Iron