title: Cofilin Phosphorylation Decreased by Serum-free Starvation with Low Glucose in the L6 Myoblasts.
abstract: [Purpose] Many studies have been using cell culture models of muscle cells with exogenous cytokines or glucocorticoids to mimic atrophy in in vivo and in vitro tests. However, the changes in the phosphorylation of atrophy-related cofilin are still poorly understood in starved skeletal muscle cells. In this study, we first examined whether or not phosphorylation of cofilin is altered in L6 myoblasts after 3, 6, 12, 24, 48, and 72 hours of serum-free starvation with low glucose. [Methods] We used Western blotting to exam protein expression and phosphorylation in atrophied L6 myoblasts. [Results] L6 cell sizes and numbers were diminished as a result of serum-free starvation in a time-dependent manner. Serum-free starvation for 3, 6, 12, 24, 48, and 72 hours significantly decreased the phosphorylation of cofilin, respectively. [Conclusion] These results suggest that starvation-induced atrophy may be in part related to changes in the phosphorylation of cofilin in L6 myoblasts.
authors.affiliation: Department of Physical Therapy, Laboratory of Health Science and Nanophysiotherapy, Yongin University, Republic of Korea ; Department of Physical Therapy, Laboratory of Health Science and Nanophysiotherapy, Yongin University, Republic of Korea.
authors.forename: Mee-Young
authors.initials: MY
authors.lastname: Kim
authors.affiliation: Department of Physical Therapy, Laboratory of Health Science and Nanophysiotherapy, Yongin University, Republic of Korea.
authors.forename: Ju-Hyun
authors.initials: JH
authors.lastname: Kim
authors.affiliation: Department of Physical Therapy, Laboratory of Health Science and Nanophysiotherapy, Yongin University, Republic of Korea.
authors.forename: Jeong-Uk
authors.initials: JU
authors.lastname: Lee
authors.affiliation: Department of Physical Therapy, Laboratory of Health Science and Nanophysiotherapy, Yongin University, Republic of Korea.
authors.forename: Lim-Kyu
authors.initials: LK
authors.lastname: Lee
authors.affiliation: Department of Physical Therapy, Laboratory of Health Science and Nanophysiotherapy, Yongin University, Republic of Korea.
authors.forename: Seung-Min
authors.initials: SM
authors.lastname: Yang
authors.affiliation: Department of Physical Therapy, Laboratory of Health Science and Nanophysiotherapy, Yongin University, Republic of Korea.
authors.forename: Byoung-Sun
authors.initials: BS
authors.lastname: Park
authors.affiliation: Department of Physical Therapy, Laboratory of Health Science and Nanophysiotherapy, Yongin University, Republic of Korea.
authors.forename: Hye-Joo
authors.initials: HJ
authors.lastname: Jeon
authors.affiliation: Department of Physical Therapy, Laboratory of Health Science and Nanophysiotherapy, Yongin University, Republic of Korea.
authors.forename: Won-Deok
authors.initials: WD
authors.lastname: Lee
authors.affiliation: Department of Physical Therapy, Laboratory of Health Science and Nanophysiotherapy, Yongin University, Republic of Korea.
authors.forename: Ji-Woong
authors.initials: JW
authors.lastname: Noh
authors.affiliation: Department of Taekwondo Instructor Education, College of Martial Arts, Yongin University, Republic of Korea.
authors.forename: Taek-Yong
authors.initials: TY
authors.lastname: Kwak
authors.affiliation: Department of Judo, College of Martial Arts, Yongin University, Republic of Korea.
authors.forename: Sung-Ho
authors.initials: SH
authors.lastname: Jang
authors.affiliation: Combative Martial Arts Training, College of Martial Arts, Yongin University, Republic of Korea.
authors.forename: Tae-Hyun
authors.initials: TH
authors.lastname: Lee
authors.affiliation: Combative Martial Arts Training, College of Martial Arts, Yongin University, Republic of Korea.
authors.forename: Ju-Young
authors.initials: JY
authors.lastname: Kim
authors.affiliation: Department of Sports Science and Engineering, Korea Institute of Sport Science, Republic of Korea.
authors.forename: Tae-Whan
authors.initials: TW
authors.lastname: Kim
authors.affiliation: Department of Physiology, School of Medicine, Institute of Functional Genomics, Konkuk University, Republic of Korea.
authors.forename: Bokyung
authors.initials: B
authors.lastname: Kim
authors.affiliation: Department of Physical Therapy, College of Public Health and Welfare, Yongin University, Republic of Korea.
authors.forename: Junghwan
authors.initials: J
authors.lastname: Kim
keywords: Cofilin; L6 myoblasts; Serum-free starvation