title: Carboxyl-ester lipase maturity-onset diabetes of the young disease protein biomarkers in secretin-stimulated duodenal juice.
abstract: Patients with carboxyl-ester lipase-maturity-onset diabetes of the young (CEL-MODY) display distinct disease stages toward the development of monogenic diabetes and exocrine pancreatic disease. The finding of differentially increased proteins, some related to MAPK signaling, in a discovery proteomics study of secretin-stimulated duodenal juice in three CEL-MODY patients, prompted us to monitor their abundance in an extensive number of CEL-MODY subjects at different disease stages and controls using targeted proteomics. In the current study, we demonstrate the feasibility of selected reaction monitoring assays to quantify protein levels in secretin-stimulated duodenal juice. Furthermore, we define a set of five peptides for potential use as diagnostic tests in CEL-MODY patients. Finally, we propose a further set of seven proteins with a likely pathogenic role in CEL-MODY disease progression.
authors.affiliation: KG Jebsen Center for Diabetes Research, Department of Clinical Science, University of Bergen , Jonas Lies Vei 65, Bergen 5021, Norway.
authors.forename: Yngvild
authors.initials: Y
authors.lastname: Bjorlykke
authors.forename: Heidrun
authors.initials: H
authors.lastname: Vethe
authors.forename: Marc
authors.initials: M
authors.lastname: Vaudel
authors.forename: Harald
authors.initials: H
authors.lastname: Barsnes
authors.forename: Frode S
authors.initials: FS
authors.lastname: Berven
authors.forename: Erling
authors.initials: E
authors.lastname: Tjora
authors.forename: Helge
authors.initials: H
authors.lastname: Raeder
mesh_terms: D015415:Biomarkers / Q000378:metabolism*; D043182:Carboxylesterase / Q000378:metabolism*; D003924:Diabetes Mellitus, Type 2 / Q000175:diagnosis / Q000378:metabolism*; D004386:Duodenum / Q000378:metabolism*; D006801:Humans; D007419:Intestinal Secretions / Q000378:metabolism*; D007553:Isotope Labeling; D013058:Mass Spectrometry; D020543:Proteome / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*; D018709:Statistics, Nonparametric; D000516:alpha-Amylases / Q000378:metabolism
chemical_list: D015415:Biomarkers; D020543:Proteome; D043182:Carboxylesterase; D000516:alpha-Amylases
keywords: CEL-MODY; biomarker; diabetes; duodenal juice; mass spectrometry; proteomics; selected reaction monitoring