title: Isolation, chemical characterization, and free radical scavenging activity of phenolics from Triticum aestivum L. aerial parts.
abstract: Fourteen phenolic compounds (flavonoids and phenolic acids) were isolated and 19 were identified in the aerial parts of Triticum aestivum L. The structures of these compounds were established on the basis of the data obtained by electrospray ionization-mass spectrometry (ESI-MS) and nuclear magnetic resonance (NMR) techniques. T. aestivum L. was found to be rich in flavones, especially in luteolin derivatives. Three of the isolated compounds, including luteolin 6-C-[6Glc″-O-E-caffeoyl-β-D-glucopyranosyl(1″→2)-β-glucopyranoside], luteolin 6-C-[5Rib″-O-E-feruoyl-β-D-ribofuranosyl(1″→2)-β-glucopyranoside], and 3',4',5'-O-trimethyltricetin 7-O-[β-D-glucuropyranosyl(1″→2)-β-D-glucopyranoside], have been reported for the first time in the plant kingdom. The amount of individual phenolics, in winter wheat, was also determined. Additionally, the free radical scavenging potential of the isolated compounds was tested in a simple and rapid thin-layer chromatography-2,2-diphenyl-1-picrylhydrazyl radical test (TLC-DPPH•) with image processing.
authors.affiliation: Department of Biochemistry and Crop Quality, Institute of Soil Science and Plant Cultivation, State Research Institute , Czartoryskich 8, 24-100 Pulawy, Poland.
authors.forename: Iwona
authors.initials: I
authors.lastname: Kowalska
authors.forename: Lukasz
authors.initials: L
authors.lastname: Pecio
authors.forename: Lukasz
authors.initials: L
authors.lastname: Ciesla
authors.forename: Wieslaw
authors.initials: W
authors.lastname: Oleszek
authors.forename: Anna
authors.initials: A
authors.lastname: Stochmal
mesh_terms: D016166:Free Radical Scavengers / Q000737:chemistry* / Q000302:isolation & purification; D005609:Free Radicals / Q000737:chemistry; D010636:Phenols / Q000737:chemistry* / Q000302:isolation & purification; D035261:Plant Components, Aerial / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry* / Q000302:isolation & purification; D014908:Triticum / Q000737:chemistry*
chemical_list: D016166:Free Radical Scavengers; D005609:Free Radicals; D010636:Phenols; D010936:Plant Extracts
keywords: Sorbfil TLC Videodensitometer; Triticum aestivum L.; free radical scavengers; phenolics