title: Vigna angularis water extracts protect against ultraviolet b-exposed skin aging in vitro and in vivo.
abstract: Exposure to ultraviolet (UV) radiation induces various pathological changes, such as thickened skin and wrinkle formation. In particular, UVB irradiation increases matrix metalloproteinase (MMP)-1 production and collagen degradation, leading to premature aging, termed photoaging. The azuki bean (Vigna angularis; VA) has been widely used as a food product as well as a traditional medicine. However, its activity needs additional study to confirm its functional application in foods and cosmetics for protecting skin. In this study, hot-water extract from VA (VAE) and its active component, rutin, were investigated to determine their antiphotoaging effects. VAE was found to have antioxidant activity. In UVB-exposed normal human dermal fibroblasts cells with VAE and rutin treatments, MMP-1 production was significantly suppressed (90% and 47%, respectively). The effects of both topical and oral administration of VAE were tested in UVB-irradiated hairless mice. VAE suppressed wrinkle formation and skin thickness by promoting elastin, procollagen type I, and TGF-β1 expression (118%, 156%, and 136%, respectively) and by diminishing MMP-1 production. These results suggest that VAE may be effective for preventing skin photoaging accelerated by UVB radiation.
authors.affiliation: Department of Oriental Medicinal Material and Processing, College of Life Science, Kyung Hee University Global Campus , Yongin-si, Gyeonggi-do, Korea.
authors.forename: Eunson
authors.initials: E
authors.lastname: Hwang
authors.forename: Sang-Yong
authors.initials: SY
authors.lastname: Park
authors.forename: Hyun Ji
authors.initials: HJ
authors.lastname: Lee
authors.forename: Zheng-wang
authors.initials: ZW
authors.lastname: Sun
authors.forename: Tae Youp
authors.initials: TY
authors.lastname: Lee
authors.forename: Hyun Geun
authors.initials: HG
authors.lastname: Song
authors.forename: Heon-Sub
authors.initials: HS
authors.lastname: Shin
authors.forename: Tae Hoo
authors.initials: TH
authors.lastname: Yi
mesh_terms: D000818:Animals; D024042:Collagen Type I / Q000378:metabolism / Q000528:radiation effects; D004549:Elastin / Q000187:drug effects; D007887:Fabaceae / Q000737:chemistry*; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D020781:Matrix Metalloproteinase 1 / Q000378:metabolism; D051379:Mice; D008812:Mice, Hairless; D010936:Plant Extracts / Q000494:pharmacology*; D020011:Protective Agents / Q000008:administration & dosage / Q000494:pharmacology*; D012867:Skin / Q000187:drug effects / Q000528:radiation effects; D015595:Skin Aging / Q000187:drug effects*; D053773:Transforming Growth Factor beta1 / Q000378:metabolism / Q000528:radiation effects; D014466:Ultraviolet Rays / Q000009:adverse effects*; D014867:Water
chemical_list: D024042:Collagen Type I; D010936:Plant Extracts; D020011:Protective Agents; D053773:Transforming Growth Factor beta1; D014867:Water; D004549:Elastin; D020781:Matrix Metalloproteinase 1
keywords: MMP-1; Vigna angularis; dermal fibroblast; hairless mice; photoaging rutin