title: Investigating fluorescent dyes in fluorescence-assisted screenings.
abstract: Screening of bead-based peptide libraries against fluorescent dye-labeled target proteins was found to be significantly influenced by the dye characteristics. Commercially available red fluorescent dyes with net negative charges adversely showed strong interactions with library beads. The introduction of zwitterionic dyes significantly reduced the unwanted interactions, which sheds light upon using the right fluorescent probe for acquisition of reliable results in various fluorescence-assisted applications.
authors.affiliation: Institute of Bioengineering and Nanotechnology, 31 Biopolis Way, The Nanos, Singapore 138669. sslee@ibn.a-star.edu.sg.
authors.forename: Joo-Eun
authors.initials: JE
authors.lastname: Jee
authors.forename: Jaehong
authors.initials: J
authors.lastname: Lim
authors.forename: Hoon
authors.initials: H
authors.lastname: Hyun
authors.forename: Jessica
authors.initials: J
authors.lastname: Oon
authors.forename: Yong Siang
authors.initials: YS
authors.lastname: Ong
authors.forename: Cedrik
authors.initials: C
authors.lastname: Massif
authors.forename: Young-Tae
authors.initials: YT
authors.lastname: Chang
authors.forename: Hak Soo
authors.initials: HS
authors.lastname: Choi
authors.forename: Su Seong
authors.initials: SS
authors.lastname: Lee
mesh_terms: D002232:Carbocyanines / Q000737:chemistry; D024402:Carbonic Anhydrase II / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D019151:Peptide Library; D010455:Peptides / Q000737:chemistry
chemical_list: C569686:Alexa Fluor 647; D002232:Carbocyanines; D005456:Fluorescent Dyes; D019151:Peptide Library; D010455:Peptides; D024402:Carbonic Anhydrase II