title: Nitrogen-fixing bacteria in Eucalyptus globulus plantations.
abstract: Eucalypt cultivation is an important economic activity worldwide. In Portugal, Eucalyptus globulus plantations account for one-third of the total forested area. The nutritional requirements of this crop have been well studied, and nitrogen (N) is one of the most important elements required for vegetal growth. N dynamics in soils are influenced by microorganisms, such as diazotrophic bacteria (DB) that are responsible for biological nitrogen fixation (BNF), so the aim of this study was to evaluate and identity the main groups of DB in E. globulus plantations. Samples of soil and root systems were collected in winter and summer from three different Portuguese regions (Penafiel, Gavião and Odemira). We observed that DB communities were affected by season, N fertilization and moisture. Furthermore Bradyrhizobium and Burkholderia were the most prevalent genera in these three regions. This is the first study describing the dynamic of these bacteria in E. globulus plantations, and these data will likely contribute to a better understanding of the nutritional requirements of eucalypt cultivation and associated organic matter turnover.
authors.affiliation: Departamento de Microbiologia, Universidade Federal de Viçosa, Viçosa, Minas Gerais, Brazil.
authors.forename: Marliane de Cássia Soares
authors.initials: Mde C
authors.lastname: da Silva
authors.affiliation: Departamento de Microbiologia, Universidade Federal de Viçosa, Viçosa, Minas Gerais, Brazil.
authors.forename: Thiago de Almeida
authors.initials: Tde A
authors.lastname: Paula
authors.affiliation: Departamento de Microbiologia, Universidade Federal de Viçosa, Viçosa, Minas Gerais, Brazil.
authors.forename: Bruno Coutinho
authors.initials: BC
authors.lastname: Moreira
authors.affiliation: Faculdade de Ciências da Universidade de Lisboa, Centro de Biologia Ambiental, Lisboa, Campo Grande, Portugal.
authors.forename: Manuela
authors.initials: M
authors.lastname: Carolino
authors.affiliation: Faculdade de Ciências da Universidade de Lisboa, Centro de Biologia Ambiental, Lisboa, Campo Grande, Portugal.
authors.forename: Cristina
authors.initials: C
authors.lastname: Cruz
authors.affiliation: Departamento de Microbiologia, Universidade Federal de Viçosa, Viçosa, Minas Gerais, Brazil.
authors.forename: Denise Mara Soares
authors.initials: DM
authors.lastname: Bazzolli
authors.affiliation: Departamento de Microbiologia, Universidade Federal de Viçosa, Viçosa, Minas Gerais, Brazil.
authors.forename: Cynthia Canedo
authors.initials: CC
authors.lastname: Silva
authors.affiliation: Departamento de Microbiologia, Universidade Federal de Viçosa, Viçosa, Minas Gerais, Brazil.
authors.forename: Maria Catarina Megumi
authors.initials: MC
authors.lastname: Kasuya
mesh_terms: D001419:Bacteria / Q000378:metabolism*; D058645:Denaturing Gradient Gel Electrophoresis; D004777:Environment; D005052:Eucalyptus / Q000382:microbiology*; D005843:Geography; D009584:Nitrogen / Q000737:chemistry; D009586:Nitrogen Fixation; D018517:Plant Roots / Q000382:microbiology; D016133:Polymerase Chain Reaction; D011174:Portugal; D025341:Principal Component Analysis; D012987:Soil; D012988:Soil Microbiology; D013045:Species Specificity
chemical_list: D012987:Soil; D009584:Nitrogen