title: Silencing of E6/E7 expression in cervical cancer stem-like cells.
abstract: Accumulating evidence supports the concept that cancer stem cells (CSCs) are responsible for the tumor recurrence and metastasis, the two major causes of cancer-related death. Therefore, CSC-targeted cancer therapy is important for the future development of more effective and advanced cancer therapy. One of the approaches is to specifically silence oncogene expression in CSCs and inhibit their growth. The significance of this approach is its specificity and ability to avoid multi-drug resistance of CSCs. In this chapter, we will describe a method of silencing HPV oncogenes E6/E7 in human cervical CSCs using HeLa cells as a model system.
authors.affiliation: Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, Corner College and Cooper Roads (Building 75), Brisbane, St. Lucia, QLD, 4072, Australia.
authors.forename: Wenyi
authors.initials: W
authors.lastname: Gu
authors.forename: Nigel
authors.initials: N
authors.lastname: McMillan
authors.forename: Chengzhong
authors.initials: C
authors.lastname: Yu
mesh_terms: D002452:Cell Count; D002469:Cell Separation; D048429:Cell Size; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D005260:Female; D020868:Gene Silencing; D006367:HeLa Cells; D006801:Humans; D016086:Lentivirus / Q000378:metabolism; D014411:Neoplastic Stem Cells / Q000378:metabolism*; D009856:Oncogene Proteins, Viral / Q000235:genetics* / Q000378:metabolism; D009857:Oncogenes; D034741:RNA, Small Interfering / Q000378:metabolism; D018874:Spheroids, Cellular / Q000378:metabolism; D014161:Transduction, Genetic; D002583:Uterine Cervical Neoplasms / Q000378:metabolism* / Q000473:pathology*
chemical_list: D004268:DNA-Binding Proteins; C052603:E6 protein, Human papillomavirus type 18; C052633:E7 protein, Human papillomavirus type 18; D009856:Oncogene Proteins, Viral; D034741:RNA, Small Interfering