title: The sustained-release behavior and in vitro and in vivo transfection of pEGFP-loaded core-shell-structured chitosan-based composite particles.
abstract: Novel submicron core-shell-structured chitosan-based composite particles encapsulated with enhanced green fluorescent protein plasmids (pEGFP) were prepared by complex coacervation method. The core was pEGFP-loaded thiolated N-alkylated chitosan (TACS) and the shell was pH- and temperature-responsive hydroxybutyl chitosan (HBC). pEGFP-loaded TACS-HBC composite particles were spherical, and had a mean diameter of approximately 120 nm, as measured by transmission electron microscopy and particle size analyzer. pEGFP showed sustained release in vitro for >15 days. Furthermore, in vitro transfection in human embryonic kidney 293T and human cervix epithelial cells, and in vivo transfection in mice skeletal muscle of loaded pEGFP, were investigated. Results showed that the expression of loaded pEGFP, both in vitro and in vivo, was slow but could be sustained over a long period. pEGFP expression in mice skeletal muscle was sustained for >60 days. This work indicates that these submicron core-shell-structured chitosan-based composite particles could potentially be used as a gene vector for in vivo controlled gene transfection.
authors.affiliation: Department of Plastic Surgery, First Affiliated Hospital of Anhui Medical University, Hefei, Anhui, People's Republic of China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Wang
authors.affiliation: CAS Key Laboratory of Soft Matter Chemistry, Department of Polymer Science and Engineering, University of Science and Technology of China, Hefei, Anhui, People's Republic of China.
authors.forename: Fu-xing
authors.initials: FX
authors.lastname: Lin
authors.affiliation: Department of Plastic Surgery, First Affiliated Hospital of Anhui Medical University, Hefei, Anhui, People's Republic of China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: CAS Key Laboratory of Soft Matter Chemistry, Department of Polymer Science and Engineering, University of Science and Technology of China, Hefei, Anhui, People's Republic of China.
authors.forename: Mo-zhen
authors.initials: MZ
authors.lastname: Wang
authors.affiliation: CAS Key Laboratory of Soft Matter Chemistry, Department of Polymer Science and Engineering, University of Science and Technology of China, Hefei, Anhui, People's Republic of China.
authors.forename: Xue-wu
authors.initials: XW
authors.lastname: Ge
authors.affiliation: Department of Plastic Surgery, First Affiliated Hospital of Anhui Medical University, Hefei, Anhui, People's Republic of China.
authors.forename: Zheng-xing
authors.initials: ZX
authors.lastname: Gong
authors.affiliation: Department of Plastic Surgery, First Affiliated Hospital of Anhui Medical University, Hefei, Anhui, People's Republic of China.
authors.forename: Dan-dan
authors.initials: DD
authors.lastname: Bao
authors.affiliation: Department of Plastic Surgery, First Affiliated Hospital of Anhui Medical University, Hefei, Anhui, People's Republic of China.
authors.forename: Yu-fang
authors.initials: YF
authors.lastname: Gu
mesh_terms: D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D048271:Chitosan / Q000737:chemistry*; D004247:DNA / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D003692:Delayed-Action Preparations / Q000737:chemistry* / Q000633:toxicity; D049452:Green Fluorescent Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D057809:HEK293 Cells; D006367:HeLa Cells; D006801:Humans; D008297:Male; D051379:Mice; D053758:Nanoparticles / Q000737:chemistry* / Q000633:toxicity; D010957:Plasmids / Q000235:genetics; D014162:Transfection / Q000379:methods*
chemical_list: D003692:Delayed-Action Preparations; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins; D004247:DNA; D048271:Chitosan
keywords: complex coacervation; gene therapy; gene transfection; hydroxybutyl chitosan; pEGFP; thiolated N-alkylated chitosan