title: Piceatannol and its metabolite, isorhapontigenin, induce SIRT1 expression in THP-1 human monocytic cell line.
abstract: Piceatannol is a phytochemical that is present in large amounts in passion fruit (Passiflora edulis) seeds, and is an analog of resveratrol. Recently, the absorption and metabolism of piceatannol were investigated in rats, and isorhapontigenin, O-methyl piceatannol, was detected as a piceatannol metabolite in rat plasma. To elucidate the function of piceatannol and its metabolites, we investigated the expression of sirtuin 1 (SIRT1) in THP-1 monocytic cells after treatment with piceatannol and its metabolites, and compared their effects with those of resveratrol and its metabolites. Piceatannol and resveratrol upregulated the expression levels of SIRT1 mRNA and SIRT1 protein. An extract of passion fruit seeds, which contained high levels of piceatannol, also upregulated SIRT1 mRNA expression. As for the metabolites, isorhapontigenin upregulated SIRT1 mRNA expression, whereas resveratrol glucuronides and sulfate did not affect SIRT1 expression. These findings indicate that after intake of piceatannol, not only piceatannol itself, but also its metabolite, isorhapontigenin, contributed to the upregulation of SIRT1 expression.
authors.affiliation: Research Institute, Morinaga and Company Limited, 2-1-1 Shimosueyoshi, Tsurumi-ku, Yokohama 230-8504, Japan. s-kawakami-jf@morinaga.co.jp.
authors.forename: Shinpei
authors.initials: S
authors.lastname: Kawakami
authors.affiliation: Research Institute, Morinaga and Company Limited, 2-1-1 Shimosueyoshi, Tsurumi-ku, Yokohama 230-8504, Japan. y-kinoshita-jg@morinaga.co.jp.
authors.forename: Yosuke
authors.initials: Y
authors.lastname: Kinoshita
authors.affiliation: Research Institute, Morinaga and Company Limited, 2-1-1 Shimosueyoshi, Tsurumi-ku, Yokohama 230-8504, Japan. h-uchida-ji@morinaga.co.jp.
authors.forename: Hiroko
authors.initials: H
authors.lastname: Maruki-Uchida
authors.affiliation: Research Institute, Morinaga and Company Limited, 2-1-1 Shimosueyoshi, Tsurumi-ku, Yokohama 230-8504, Japan. k-yanae-ja@morinaga.co.jp.
authors.forename: Koji
authors.initials: K
authors.lastname: Yanae
authors.affiliation: Research Institute, Morinaga and Company Limited, 2-1-1 Shimosueyoshi, Tsurumi-ku, Yokohama 230-8504, Japan. m-sai-ia@morinaga.co.jp.
authors.forename: Masahiko
authors.initials: M
authors.lastname: Sai
authors.affiliation: Research Institute, Morinaga and Company Limited, 2-1-1 Shimosueyoshi, Tsurumi-ku, Yokohama 230-8504, Japan. t-ito-jj@morinaga.co.jp.
authors.forename: Tatsuhiko
authors.initials: T
authors.lastname: Ito
mesh_terms: D002460:Cell Line; D006801:Humans; D009000:Monocytes / Q000187:drug effects / Q000378:metabolism*; D029598:Passiflora / Q000737:chemistry; D010936:Plant Extracts / Q000494:pharmacology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D000077185:Resveratrol; D012639:Seeds / Q000737:chemistry; D056564:Sirtuin 1 / Q000235:genetics / Q000378:metabolism*; D013267:Stilbenes / Q000494:pharmacology*; D015854:Up-Regulation
chemical_list: D010936:Plant Extracts; D012333:RNA, Messenger; D013267:Stilbenes; C432307:isorhapontigenin; C041525:3,3',4,5'-tetrahydroxystilbene; C447939:SIRT1 protein, human; D056564:Sirtuin 1; D000077185:Resveratrol