title: Profile of microRNA expression in brain tumors of different malignancy.
abstract: The expression profiles of 10 mature microRNA (7, 10a, 17, 20a, 21, 23a, 26a, 137, 222) in biopsy specimens of gliomas of different malignancy were studied by the real time PCR with SYBR Green I fluorescent stain. The expression of microRNA-21 increased significantly, while that of microRNA-137 decreased, depending on the tumor malignancy. The expression of microRNA-9, -17, -20a, -23a, -26a was significantly higher, while that of microRNA-7 lower in the tumor vs. control tissue samples. New data on the molecular pathological mechanisms of gliomas related to their malignancy were obtained. Quantitative analysis of microRNA was suggested as a potential diagnostic marker of brain tumors.
authors.affiliation: Department of Medical Nanobiotechnologies, N. I. Pirogov Russian Research Medical University, the Ministry of Health of the Russian Federation, Moscow, Russia, philipkoshkin@gmail.com.
authors.forename: F A
authors.initials: FA
authors.lastname: Koshkin
authors.forename: D A
authors.initials: DA
authors.lastname: Chistyakov
authors.forename: A G
authors.initials: AG
authors.lastname: Nikitin
authors.forename: A N
authors.initials: AN
authors.lastname: Konovalov
authors.forename: A A
authors.initials: AA
authors.lastname: Potapov
authors.forename: D Yu
authors.initials: DY
authors.lastname: Usachyov
authors.forename: D I
authors.initials: DI
authors.lastname: Pitskhelauri
authors.forename: G L
authors.initials: GL
authors.lastname: Kobyakov
authors.forename: L V
authors.initials: LV
authors.lastname: Shishkina
authors.forename: V P
authors.initials: VP
authors.lastname: Chekhonin
mesh_terms: D052160:Benzothiazoles; D014408:Biomarkers, Tumor / Q000378:metabolism; D017931:DNA Primers / Q000235:genetics; D003959:Diamines; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics*; D005910:Glioma / Q000378:metabolism*; D006801:Humans; D035683:MicroRNAs / Q000378:metabolism*; D009930:Organic Chemicals; D011804:Quinolines; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D018709:Statistics, Nonparametric; D059467:Transcriptome
chemical_list: D052160:Benzothiazoles; D014408:Biomarkers, Tumor; D017931:DNA Primers; D003959:Diamines; D035683:MicroRNAs; D009930:Organic Chemicals; D011804:Quinolines; C098022:SYBR Green I