title: Synergistic coupling of fluorescent "turn-off" with spectral overlap modulated FRET for ratiometric Ag+ sensor.
abstract: A useful strategy for ratiometric fluorescent detecting of Ag(+) is demonstrated. Upon selective binding of Ag(+) to a BODIPY-porphyrin dyad (1), the synergistic coupling of two functions, namely the suppressing of FRET from BODIPY donor to porphyrin acceptor and the fluorescence quenching of porphyrin acceptor, leads to exceptionally large changes in the intensity ratio of two distinct emissions (F513/F654) which allow for the ratiometric detecting of Ag(+) with excellent sensitivity in solution and living cells.
authors.affiliation: Beijing Key Laboratory for Science and Application of Functional Molecular and Crystalline Materials, Department of Chemistry, University of Science and Technology Beijing , Beijing 100083, China.
authors.forename: Mengliang
authors.initials: M
authors.lastname: Zhu
authors.forename: Yabin
authors.initials: Y
authors.lastname: Zhou
authors.forename: Liguo
authors.initials: L
authors.lastname: Yang
authors.forename: Lin
authors.initials: L
authors.lastname: Li
authors.forename: Dongdong
authors.initials: D
authors.lastname: Qi
authors.forename: Ming
authors.initials: M
authors.lastname: Bai
authors.forename: Yuting
authors.initials: Y
authors.lastname: Chen
authors.forename: Hongwu
authors.initials: H
authors.lastname: Du
authors.forename: Yongzhong
authors.initials: Y
authors.lastname: Bian
mesh_terms: D001896:Boron Compounds / Q000737:chemistry*; D018929:Cell Culture Techniques; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry* / Q000633:toxicity; D006801:Humans; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D057054:Molecular Imaging / Q000379:methods; D011166:Porphyrins / Q000737:chemistry*; D012680:Sensitivity and Specificity; D018030:Silver Compounds / Q000032:analysis*
chemical_list: C095489:4,4-difluoro-4-bora-3a,4a-diaza-s-indacene; D001896:Boron Compounds; D005456:Fluorescent Dyes; D011166:Porphyrins; D018030:Silver Compounds