title: Hsp25 and Hsp72 content in rat skeletal muscle following controlled shortening and lengthening contractions.
abstract: The cytoprotective proteins, Hsp25 and Hsp72, are increased in skeletal muscle after nondamaging, shortening contractions, but the temporal pattern of expression and stimulatory mechanisms remain unclear. Thus, we sought to define the in vivo temporal patterns of expression for Hsp25 and Hsp72 after 2 opposing contractions types. To do this, male Sprague-Dawley rats had 1 tibialis anterior (TA) muscle electrically stimulated (5 sets of 20 repetitions) while being either forcibly lengthened (LC) or shortened (SC). At 2, 8, 24, 48, 72, or 168 h after the contractions both the stimulated and the nonstimulated (contra-lateral control) TA muscles were removed and processed to examine muscle damage (hemotoxylin and eosin staining) and Hsp content (Western blot analyses). Cross-sections from TA muscles subjected to LCs showed muscle fibre damage at 8 h and thereafter. In contrast, no muscle fibre damage was observed at any time point following SCs. When normalized to contra-lateral controls, Hsp25 and Hsp72 content were significantly (P < 0.01) increased at 24 h (3.1- and 3.8-fold, respectively) and thereafter. There were no significant increases in Hsp25 or Hsp72 content at any time point following SC. These data suggest that LCs, but not SCs, result in Hsp accumulation and that the fibre/cellular damage sustained from LCs may be the stimulus for elevating Hsp content.
authors.affiliation: Faculty of Kinesiology and Physical Education, University of Toronto, Toronto, ON M5S 2W6, Canada.
authors.forename: Andrew M
authors.initials: AM
authors.lastname: Holwerda
authors.forename: Marius
authors.initials: M
authors.lastname: Locke
mesh_terms: D000818:Animals; D055551:HSP27 Heat-Shock Proteins / Q000032:analysis*; D050884:HSP72 Heat-Shock Proteins / Q000032:analysis*; D008297:Male; D009119:Muscle Contraction; D018482:Muscle, Skeletal / Q000737:chemistry* / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D055551:HSP27 Heat-Shock Proteins; D050884:HSP72 Heat-Shock Proteins; C486114:Hspb1 protein, rat
keywords: Hsp25; Hsp72; concentric; contraction musculaire; eccentric; lésion musculaire; miométrique; muscle contraction; muscle damage; pliométrique