title: Dark-field-based observation of single-nanoparticle dynamics on a supported lipid bilayer for in situ analysis of interacting molecules and nanoparticles.
abstract: Observation of single plasmonic nanoparticles in reconstituted biological systems allows us to obtain snapshots of dynamic processes between molecules and nanoparticles with unprecedented spatiotemporal resolution and single-molecule/single-particle-level data acquisition. This Concept is intended to introduce nanoparticle-tethered supported lipid bilayer platforms that allow for the dynamic confinement of nanoparticles on a two-dimensional fluidic surface. The dark-field-based long-term, stable, real-time observation of freely diffusing plasmonic nanoparticles on a lipid bilayer enables one to extract a broad range of information about interparticle and molecular interactions throughout the entire reaction period. Herein, we highlight important developments in this context to provide ideas on how molecular interactions can be interpreted by monitoring dynamic behaviors and optical signals of laterally mobile nanoparticles.
authors.affiliation: Department of Chemistry, Seoul National University, Seoul 151-747 (South Korea); Howard Hughes Medical Institute and Department of Chemistry, University of California, Berkeley, CA 94720 (USA).
authors.forename: Young Kwang
authors.initials: YK
authors.lastname: Lee
authors.forename: Sungi
authors.initials: S
authors.lastname: Kim
authors.forename: Jwa-Min
authors.initials: JM
authors.lastname: Nam
mesh_terms: D004058:Diffusion; D004867:Equipment Design; D008051:Lipid Bilayers / Q000032:analysis / Q000378:metabolism*; D008853:Microscopy / Q000295:instrumentation / Q000379:methods; D008958:Models, Molecular; D053758:Nanoparticles / Q000032:analysis / Q000378:metabolism* / Q000648:ultrastructure; D036103:Nanotechnology
chemical_list: D008051:Lipid Bilayers
keywords: in situ analysis; lipid bilayers; nanoparticles; plasmonic nanoparticles; single-molecule studies