title: Spread of Streptococcus pneumoniae serotype 8-ST63 multidrug-resistant recombinant Clone, Spain.
abstract: Since 2004, a total of 131 isolates of Streptococcus pneumoniae multidrug-resistant invasive serotype 8 have been detected in Spain. These isolates showed resistance to erythromycin, clindamycin, tetracycline, and ciprofloxacin. All isolates were obtained from adult patients and shared a common genotype (sequence type [ST]63; penicillin-binding protein 1a [pbp1a], pbp2b, and pbp2x gene profiles; ermB and tetM genes; and a ParC-S79F change). Sixty-eight isolates that required a ciprofloxacin MIC ≥16 μg/mL had additional gyrA gene changes. Serotype 8-ST63 pbp2x sequences were identical with those of antimicrobial drug-susceptible serotype 8-ST53 isolates. Serotype 8-ST63 pbp2b sequences were identical with those of the multidrug-resistant Sweden 15A-ST63 clone. Recombination between the capsular locus and flanking regions of an ST53 isolate (donor) and an ST63 pneumococcus (recipient) generated the novel 15A-ST63 clone. One recombination point was upstream of pbp2x and another was within pbp1a. A serotype 8-ST63 clone was identified as a cause of invasive disease in Spain.
authors.forename: Carmen
authors.initials: C
authors.lastname: Ardanuy
authors.forename: Adela G
authors.initials: AG
authors.lastname: de la Campa
authors.forename: Ernesto
authors.initials: E
authors.lastname: García
authors.forename: Asunción
authors.initials: A
authors.lastname: Fenoll
authors.forename: Laura
authors.initials: L
authors.lastname: Calatayud
authors.forename: Emilia
authors.initials: E
authors.lastname: Cercenado
authors.forename: Emilio
authors.initials: E
authors.lastname: Pérez-Trallero
authors.forename: Emilio
authors.initials: E
authors.lastname: Bouza
authors.forename: Josefina
authors.initials: J
authors.lastname: Liñares
mesh_terms: D000293:Adolescent; D000328:Adult; D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics; D001483:Base Sequence; D002648:Child; D003062:Codon; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics; D023061:Gene Order; D016680:Genome, Bacterial; D005838:Genotype; D006801:Humans; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D058889:Molecular Typing; D010641:Phenotype; D011008:Pneumococcal Infections / Q000453:epidemiology* / Q000382:microbiology*; D011110:Polymorphism, Genetic; D011995:Recombination, Genetic; D065288:Serogroup; D013030:Spain / Q000453:epidemiology; D013296:Streptococcus pneumoniae / Q000145:classification* / Q000235:genetics; D055815:Young Adult
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D003062:Codon