title: The avian embryo as a model system for skeletal myogenesis.
abstract: This review will focus on the use of the chicken and quail as model systems to analyze myogenesis and as such will emphasize the experimental approaches that are strongest in these systems-the amenability of the avian embryo to manipulation and in ovo observation. During somite differentiation, a wide spectrum of developmental processes occur such as cellular differentiation, migration, and fusion. Cell lineage studies combined with recent advancements in cell imaging allow these biological phenomena to be readily observed and hypotheses tested extremely rapidly-a strength that is restricted to the avian system. A clear weakness of the chicken in the past has been genetic approaches to modulate gene function. Recent advances in the electroporation of expression vectors, siRNA constructs, and use of tissue specific reporters have opened the door to increasingly sophisticated experiments that address questions of interest not only to the somite/muscle field in particular but also fundamental to biology in general. Importantly, an ever-growing body of evidence indicates that somite differentiation in birds is indistinguishable to that of mammals; therefore, these avian studies complement the complex genetic models of the mouse.
authors.affiliation: EMBL Australia, Australian Regenerative Medicine Institute (ARMI), Monash University, Clayton, VIC, 3800, Australia, claire.hirst@monash.edu.
authors.forename: Claire E
authors.initials: CE
authors.lastname: Hirst
authors.forename: Christophe
authors.initials: C
authors.lastname: Marcelle
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000235:genetics*; D019070:Cell Lineage / Q000235:genetics; D002642:Chick Embryo; D018507:Gene Expression Regulation, Developmental; D051379:Mice; D008954:Models, Biological; D024510:Muscle Development / Q000235:genetics*; D011784:Quail / Q000254:growth & development; D019170:Somites / Q000254:growth & development*