title: Perineural pretreatment of bee venom attenuated the development of allodynia in the spinal nerve ligation injured neuropathic pain model; an experimental study.
abstract: BACKGROUND
  Diluted bee venom (BV) is known to have anti-nociceptive and anti-inflammatory effects. We therefore assessed whether perineural bee venom pretreatment could attenuate the development of neuropathic pain in the spinal nerve ligation injured animal model.
  
  
  METHODS
  Neuropathic pain was surgically induced in 30 male Sprague Dawley rats by ligation of the L5 and L6 spinal nerves, with 10 rats each treated with saline and 0.05 and 0.1 mg BV. Behavioral testing for mechanical, cold, and thermal allodynia was conducted on postoperative days 3 to 29. Three rats in each group and 9 sham operated rats were sacrificed on day 9, and the expression of transient receptor potential vanilloid type 1 (TRPV1), ankyrin type 1 (TRPA1), and melastatin type 8 (TRPM8) receptors in the ipsilateral L5 dorsal root ganglion was analyzed.
  
  
  RESULTS
  The perineural administration of BV to the spinal nerves attenuated the development of mechanical, thermal, and cold allodynia, and the BV pretreatment reduced the expression of TRPV1, TRPA1, TRPM8 and c - Fos in the ipsilateral dorsal root ganglion.
  
  
  CONCLUSIONS
  The current study demonstrates that the perineural pretreatment with diluted bee venom before the induction of spinal nerve ligation significantly suppresses the development of neuropathic pain. Furthermore, this bee venom induced suppression was strongly related with the involvement of transient receptor potential family members.
authors.forename: Won Uk
authors.initials: WU
authors.lastname: Koh
authors.forename: Seong Soo
authors.initials: SS
authors.lastname: Choi
authors.forename: Jong Hyuk
authors.initials: JH
authors.lastname: Lee
authors.forename: So Hee
authors.initials: SH
authors.lastname: Lee
authors.forename: Sun Kyung
authors.initials: SK
authors.lastname: Lee
authors.forename: Yoon Kyung
authors.initials: YK
authors.lastname: Lee
authors.forename: Jeong Gil
authors.initials: JG
authors.lastname: Leem
authors.affiliation: Department of Anesthesiology and Pain Medicine, Asan Medical Center, University of Ulsan, College of Medicine, Pungnap-2Dong, Songpa-Gu, Seoul 388-1, Korea. jaljary@naver.com.
authors.forename: Jun Gol
authors.initials: JG
authors.lastname: Song
authors.forename: Jin Woo
authors.initials: JW
authors.lastname: Shin
mesh_terms: D000818:Animals; D001514:Bee Venoms / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use*; D004195:Disease Models, Animal; D005727:Ganglia, Spinal / Q000187:drug effects* / Q000378:metabolism; D006930:Hyperalgesia / Q000209:etiology / Q000378:metabolism / Q000517:prevention & control*; D008026:Ligation; D008161:Lumbosacral Region; D008297:Male; D009437:Neuralgia / Q000209:etiology / Q000378:metabolism / Q000517:prevention & control*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013127:Spinal Nerves / Q000187:drug effects* / Q000293:injuries / Q000378:metabolism; D050051:Transient Receptor Potential Channels / Q000378:metabolism
chemical_list: D001514:Bee Venoms; D050051:Transient Receptor Potential Channels