title: Non-(13)CO2 targeted breath tests: a feasibility study.
abstract: Breath tests allow a non-invasive and fast diagnostic of different specific enzymes' phenotypic functionality. Over the last decade several 13C-breath tests were successfully tested, with the (13)C-urea breath test being approved by the US Food and Drug Administration (FDA) and the European Medicines Agency (EMA). The use of other targets than labeled (13)CO2 in exhaled breath offers additional possibilities. High sensitivity analytical technologies, such as proton-transfer reaction time-of-flight mass spectrometry, enable the detection of different volatile targets in the low ppb (parts per billion) range in real-time.In the current study volunteers received 0.8 mg deuterated 2-propanol, which was converted to d3-acetone (m/z 62.08) by alcohol dehydrogenase. D3-acetone (m/z 62.08) appeared in exhaled breath concentrations up to 30 ppb (at maximum). Parallel consumption of ethanol seems to reduce the activity of the enzyme, resulting in approximately 15-30% reduction of the produced d3-acetone. Phenotypic determination of enzyme activities is important, since the functionality of enzymes is influenced by factors such as age, sex, life-style, diet, organ function, metabolism, etc, which cannot be entirely accounted for by genetic factors.
authors.affiliation: Department of Anesthesiology and Critical Care Medicine, Innsbruck Medical University, Anichstr 35, A-6020 Innsbruck, Austria. Breath Research Institute of the University of Innsbruck, Rathausplatz 4, A-6850 Dornbirn, Austria.
authors.forename: Veronika
authors.initials: V
authors.lastname: Ruzsanyi
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Lederer
authors.forename: Christoph
authors.initials: C
authors.lastname: Seger
authors.forename: Bogdan
authors.initials: B
authors.lastname: Calenic
authors.forename: Klaus R
authors.initials: KR
authors.lastname: Liedl
authors.forename: Anton
authors.initials: A
authors.lastname: Amann
mesh_terms: D019840:2-Propanol / Q000008:administration & dosage / Q000494:pharmacology; D000096:Acetone / Q000032:analysis; D001944:Breath Tests / Q000379:methods*; D002245:Carbon Dioxide; D002247:Carbon Isotopes; D000431:Ethanol / Q000032:analysis; D045853:Exhalation / Q000187:drug effects; D005240:Feasibility Studies; D006801:Humans; D057230:Limit of Detection
chemical_list: D002247:Carbon Isotopes; D000096:Acetone; D002245:Carbon Dioxide; D000431:Ethanol; D019840:2-Propanol