title: Screening of drugs of abuse and toxic compounds in human whole blood using online solid-phase extraction and high-performance liquid chromatography with time-of-flight mass spectrometry.
abstract: A novel method for the screening of 151 drugs of abuse and toxic compounds in human whole blood has been developed and validated by online solid-phase extraction with liquid chromatography coupled to time-of-flight mass spectrometry. Analytes were extracted and separated by using a fully automated online solid-phase extraction liquid chromatography system with total chromatographic run time of 26 min. Time-of-flight mass spectrometry screening of 151 drugs of abuse and toxic compounds was performed in a full-scan (m/z 50-800) mode using an MS(E) acquisition of molecular ions and fragment ions data at two collision energies (one was 6 eV and another one was in the range of 5-45 eV). The compounds were identified based on retention times and exact mass of molecular ions and fragment ions. The limit of detection ranged from 1 to 100 ng/mL and the recovery of the method ranged from 6.3 to 163.5%. This method is proved to be a valuable screening method allowing fast and specific identification of drugs in human whole blood.
authors.affiliation: State Key Laboratory of New Drug and Pharmaceutical Process, Shanghai Institute of Pharmaceutical Industry, Shanghai, P. R. China; Shanghai Key Laboratory of Crime Scene Evidence, Shanghai Institute of Forensic Science, Shanghai, P. R. China.
authors.forename: Xiaomei
authors.initials: X
authors.lastname: Teng
authors.forename: Chen
authors.initials: C
authors.lastname: Liang
authors.forename: Rong
authors.initials: R
authors.lastname: Wang
authors.forename: Tao
authors.initials: T
authors.lastname: Sun
authors.forename: Yulan
authors.initials: Y
authors.lastname: Rao
authors.forename: Chunfang
authors.initials: C
authors.lastname: Ni
authors.forename: Libo
authors.initials: L
authors.lastname: Zeng
authors.forename: Lingjuan
authors.initials: L
authors.lastname: Xiong
authors.forename: Yuan
authors.initials: Y
authors.lastname: Li
authors.forename: Yurong
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D001331:Automation / Q000379:methods; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D013287:Illicit Drugs / Q000097:blood* / Q000302:isolation & purification*; D013058:Mass Spectrometry / Q000379:methods*; D052616:Solid Phase Extraction / Q000379:methods*; D015813:Substance Abuse Detection / Q000379:methods*
chemical_list: D013287:Illicit Drugs
keywords: Drugs of abuse; Human whole blood; Online solid-phase extraction; Screening; Time-of-flight mass spectrometry