title: Lentivirus-mediated Wnt10b overexpression enhances fracture healing in a rat atrophic non-union model.
abstract: Lentivirus vectors encoding Wnt10b gene (LV-Wnt10b) or luciferase gene (LV-luc) were constructed to determine whether Wnt10b overexpression improved fracture healing in a rat atrophic non-union model. After fracture, rats were injected with LV-Wnt10b or LV-luc. Luciferase signals were clearly detected. At 2 and 4 weeks, LV-Wnt10b group had 107 and 98% more proliferating cell nuclear antigen (PCNA) positive cells, respectively, and promoted expression of bone morphogenetic protein-2 (BMP-2) in the callus compared with controls. LV-Wnt10b injection significantly increased bone mass density and bone mineral content: 46-84 and 96-193%, respectively, at the site of fracturein the LV-Wnt10b group compared with controls. At 8 weeks, fractured femora were healed in the LV-Wnt10b group compared with atrophic non-unions formed in controls. Thus, Wnt10b overexpression associated with lentiviral gene therapy is effective in healing atrophic non-unions in rats.
authors.affiliation: Department of Orthopedic Surgery, Shanghai Jiao Tong University affiliated Sixth People's Hospital, Shanghai Jiaotong University School of Medicine, Shanghai Jiaotong University, Shanghai, 200233, China, cqcps@sina.cn.
authors.forename: Feng
authors.initials: F
authors.lastname: Gao
authors.forename: Chang-qing
authors.initials: CQ
authors.lastname: Zhang
authors.forename: Yi-min
authors.initials: YM
authors.lastname: Chai
authors.forename: Xiao-lin
authors.initials: XL
authors.lastname: Li
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D004337:Drug Carriers / Q000008:administration & dosage*; D017102:Fracture Healing / Q000187:drug effects*; D050723:Fractures, Bone / Q000473:pathology / Q000628:therapy*; D015316:Genetic Therapy / Q000379:methods*; D016086:Lentivirus / Q000235:genetics*; D051381:Rats; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D016896:Treatment Outcome; D051153:Wnt Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D004337:Drug Carriers; D011994:Recombinant Proteins; D051153:Wnt Proteins; C102068:Wnt10b protein, mouse