title: Genome-wide DNA Methylation Profiles of Small Intestine and Liver in Fast-growing and Slow-growing Weaning Piglets.
abstract: Although growth rate is one of the main economic traits of concern in pig production, there is limited knowledge on its epigenetic regulation, such as DNA methylation. In this study, we conducted methyl-CpG binding domain protein-enriched genome sequencing (MBD-seq) to compare genome-wide DNA methylation profile of small intestine and liver tissue between fast- and slow-growing weaning piglets. The genome-wide methylation pattern between the two different growing groups showed similar proportion of CpG (regions of DNA where a cytosine nucleotide occurs next to a guanine nucleotide in the linear sequence) coverage, genomic regions, and gene regions. Differentially methylated regions and genes were also identified for downstream analysis. In canonical pathway analysis using differentially methylated genes, pathways (triacylglycerol pathway, some cell cycle related pathways, and insulin receptor signaling pathway) expected to be related to growth rate were enriched in the two organ tissues. Differentially methylated genes were also organized in gene networks related to the cellular development, growth, and carbohydrate metabolism. Even though further study is required, the result of this study may contribute to the understanding of epigenetic regulation in pig growth.
authors.affiliation: C&K Genomics, Seoul 151-919, Korea ; Interdisciplinary Program in Bioinformatics, Seoul National University, Seoul 151-747, Korea .
authors.forename: Woori
authors.initials: W
authors.lastname: Kwak
authors.affiliation: C&K Genomics, Seoul 151-919, Korea.
authors.forename: Jin-Nam
authors.initials: JN
authors.lastname: Kim
authors.affiliation: C&K Genomics, Seoul 151-919, Korea.
authors.forename: Daewon
authors.initials: D
authors.lastname: Kim
authors.affiliation: Department of Agricultural Biotechnology, Seoul National University, Seoul 151-921, Korea .
authors.forename: Jin Su
authors.initials: JS
authors.lastname: Hong
authors.affiliation: Department of Agricultural Biotechnology, Seoul National University, Seoul 151-921, Korea .
authors.forename: Jae Hark
authors.initials: JH
authors.lastname: Jeong
authors.affiliation: C&K Genomics, Seoul 151-919, Korea ; Interdisciplinary Program in Bioinformatics, Seoul National University, Seoul 151-747, Korea . ; Department of Agricultural Biotechnology, Seoul National University, Seoul 151-921, Korea .
authors.forename: Heebal
authors.initials: H
authors.lastname: Kim
authors.affiliation: C&K Genomics, Seoul 151-919, Korea.
authors.forename: Seoae
authors.initials: S
authors.lastname: Cho
authors.affiliation: Department of Agricultural Biotechnology, Seoul National University, Seoul 151-921, Korea .
authors.forename: Yoo Yong
authors.initials: YY
authors.lastname: Kim
keywords: DNA Methylation; Epigenetic Profile; Genome-wide Methylation Profile; MBD-seq; Weaning Piglet