title: Effect of inoculation process on lycopene production by Blakeslea trispora in a stirred-tank reactor.
abstract: Lycopene biosynthesis by Blakeslea trispora was greatly enhanced in a stirred-tank reactor when a nonsynchronous inoculation process, in which the (+) mating type was inoculated after the (-) mating type has been grown for a certain period of time, was applied. The lycopene concentration with nonsynchronous inoculation in a 24-h inoculation interval was 33 % higher than that with synchronous inoculation. The optimum inoculation ratio was 1:2 (+/-) at the 36 and 48 h inoculum age of mating types (+) and (-), respectively. Fermentation time for the individual strains and mated conditions showed that the (+) mating type grows faster than the (-) mating type. Morphological observation showed that the mycelium ratio of B. trispora (-) in mating culture with nonsynchronous inoculation was higher than that with synchronous inoculation. The results indicated that nonsynchronous inoculation process increased the dominance of B. trispora (-) in joint cultivation and hence stimulated lycopene biosynthesis.
authors.affiliation: The Key Laboratory of Industrial Biotechnology, Ministry of Education, and School of Biotechnology, Jiangnan University, Wuxi, 214122, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Wang
authors.forename: Ling-Ran
authors.initials: LR
authors.lastname: Feng
authors.forename: Wei
authors.initials: W
authors.lastname: Luo
authors.forename: Han-Guang
authors.initials: HG
authors.lastname: Li
authors.forename: Ya
authors.initials: Y
authors.lastname: Zhou
authors.forename: Xiao-Bin
authors.initials: XB
authors.lastname: Yu
mesh_terms: D018533:Biomass; D019149:Bioreactors; D002338:Carotenoids / Q000096:biosynthesis*; D005285:Fermentation; D005947:Glucose / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D000077276:Lycopene; D000072235:Mating Factor; D009090:Mucorales / Q000378:metabolism*; D025282:Mycelium / Q000378:metabolism*; D010455:Peptides / Q000502:physiology*; D013997:Time Factors
chemical_list: D010455:Peptides; D002338:Carotenoids; D000072235:Mating Factor; D005947:Glucose; D000077276:Lycopene