title: PCB77 Inducing Renal Tubular Cell Apoptosis.
abstract: PCBs are a family of persistent environmental toxicants with a wide spectrum of toxic features, such as neurotoxic, hepatoxicity, immunotoxicity, endocrine disruption effects, and oncogenic effects. The kidney is the most important organ involved in the elimination of toxins and drugs. To date, little has been done to investigate the potential influence of nephrotoxicity of 3,3',4,4'- tetrachlorobiphenyl (PCB77). By assessing cell viability and apoptotic cell death in renal tubular epithelial (NRK-52E) cells cultures, we found that PCB77 could decrease cellular viability at least at 30 μM concentration after 3 h exposure. PCB77 was demonstrated to promote DNA breakage resulting in apoptosis. Moreover, apoptotic subcellular morphological changes administration of PCB77 was observed using transmission electron microscopy. Appearance swelling of mitochondria, endoplasmic reticulum dilation and chromatin agglutinate, and other apoptosis cells morphological characteristics could be visible. Due to increased PCB77 concentration, cells viability was decreased. Collectively, our findings identified the morphological mechanism that PCB77-induced nephrotoxicity via promoting renal tubular epithelial cells apoptosis. It is suggested that using and production of PCB77 should be carefully managed to reduce public health risks.
authors.affiliation: Division of Nephrology, Renmin Hospital of Wuhan University , Wuhan, Hubei , People's Republic of China .
authors.forename: Ke
authors.initials: K
authors.lastname: Su
authors.forename: Xifeng
authors.initials: X
authors.lastname: Lv
authors.forename: Hui
authors.initials: H
authors.lastname: Song
authors.forename: Xuesong
authors.initials: X
authors.lastname: Luo
authors.forename: Cheng
authors.initials: C
authors.lastname: Chen
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D004847:Epithelial Cells / Q000187:drug effects* / Q000648:ultrastructure; D007684:Kidney Tubules / Q000187:drug effects* / Q000648:ultrastructure; D008928:Mitochondria / Q000187:drug effects; D011078:Polychlorinated Biphenyls / Q000494:pharmacology*; D051381:Rats
chemical_list: D011078:Polychlorinated Biphenyls; C028451:3,4,3',4'-tetrachlorobiphenyl
keywords: Apoptosis; PCB77; nephrotoxicity; ultrastructure