title: Sterility verification of innovative pressurized carbon dioxide lavage for bone surface preparation in cemented knee arthroplasty.
abstract: OBJECTIVE
  To verify sterility of innovative pressurized carbon dioxide (CO) lavage for bone surface preparation in cemented knee arthroplasty.
  
  
  METHODS
  Twenty-five 500 L. air samples from the innovative pressurized CO, lavage were laboratory tested to verify the presence or absence of either bacteria or fungi in microbial air samplings taken in a biological safety cabinet. CO2 gas was delivered though a pressure regulator, a sterile delivery tube, a hand piece, and a microbial air filter with a pressure level of 50 psi, according to the medical standard. Contamination samples for bacteria and fungi were taken by using Tryptic Soy Agar and SabouraudAgar as the culture media and were then analyzed at 48 hours and 10 days, respectively.
  
  
  RESULTS
  Twenty-five samples of microbial culture showed no bacterial or fungal growth on either Tryptic Soy Agar or Sabouraud Agar
  
  
  CONCLUSIONS
  The sterility of the innovative pressurized carbon dioxide lavage was confirmed by laboratory test results and was approved for application for bone surface preparation in cemented total knee arthroplasty.
authors.forename: Krit
authors.initials: K
authors.lastname: Boontanapibul
authors.forename: Pakpoom
authors.initials: P
authors.lastname: Ruangsomboon
authors.forename: Keerati
authors.initials: K
authors.lastname: Charoencholvanich
mesh_terms: D000391:Air Microbiology; D019645:Arthroplasty, Replacement, Knee; D001843:Bone Cements; D002245:Carbon Dioxide; D003666:Decontamination / Q000379:methods*; D006801:Humans; D007507:Therapeutic Irrigation / Q000379:methods*
chemical_list: D001843:Bone Cements; D002245:Carbon Dioxide