title: X-ray-induced changes in the expression of inflammation-related genes in human peripheral blood.
abstract: Using quantitative real-time polymerase chain reaction (PCR) array, we explored and compared the expression changes of inflammation-related genes in human peripheral blood irradiated with 0.5, 3, and 10 Gy doses of X-rays 24 h after exposure. Results indicated that the expression of 62 out of 84 genes was significantly altered after X-ray radiation. Among these 62 genes, 35 (such as TNFSF4) are known to be associated with radiation response, but others are novel. At a low radiation dose (0.5 Gy), 9 genes were up-regulated and 19 were down-regulated. With further increased dose to 3 Gy, 8 unique genes were up-regulated and 19 genes were down-regulated. We also identified 48 different genes that were differentially expressed significantly after 10 Gy of irradiation, and among these transcripts, up-regulated genes accounted for only one-third (16 genes) of the total. Of the 62 genes, 31 were significantly altered only at a specific dose, and a total of 10 genes were significantly expressed at all 3 doses. The dose- and time-dependent expression of CCL2 was confirmed by quantitative real-time reverse-transcription PCR. A number of candidate genes reported herein may be useful molecular biomarkers of radiation exposure in human peripheral blood.
authors.affiliation: Department of Toxicology, Henan Institute of Occupational Medicine, Zhengzhou 450052, China. wangping980623@126.com.
authors.forename: Ping
authors.initials: P
authors.lastname: Wang
authors.affiliation: College of Public Health, Zhengzhou University, Zhengzhou 450001, China. guofei_19870427@163.com.
authors.forename: Fei
authors.initials: F
authors.lastname: Guo
authors.affiliation: Department of Toxicology, Henan Institute of Occupational Medicine, Zhengzhou 450052, China. zhifangsuohanlin@126.com.
authors.forename: Lin
authors.initials: L
authors.lastname: Han
authors.affiliation: Department of Toxicology, Henan Institute of Occupational Medicine, Zhengzhou 450052, China. zhzhwxa@126.com.
authors.forename: Xi'ai
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Toxicology, Henan Institute of Occupational Medicine, Zhengzhou 450052, China. lijie1101@sina.cn.
authors.forename: Jie
authors.initials: J
authors.lastname: Li
authors.affiliation: College of Public Health, Zhengzhou University, Zhengzhou 450001, China. guoyan.208@163.com.
authors.forename: Yan
authors.initials: Y
authors.lastname: Guo
authors.affiliation: Department of Toxicology, Henan Institute of Occupational Medicine, Zhengzhou 450052, China. lym636160@sina.com.
authors.forename: Yumin
authors.initials: Y
authors.lastname: Lü
mesh_terms: D000328:Adult; D001773:Blood Cells / Q000378:metabolism; D018932:Chemokine CCL2 / Q000235:genetics; D004307:Dose-Response Relationship, Radiation; D005260:Female; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000528:radiation effects*; D006801:Humans; D007249:Inflammation / Q000235:genetics*; D008297:Male; D011829:Radiation Dosage; D015203:Reproducibility of Results; D013997:Time Factors; D014965:X-Rays
chemical_list: D018932:Chemokine CCL2