title: Continuous monitoring of arthritis in animal models using optical imaging modalities.
abstract: Given the several difficulties associated with histology, including difficulty in continuous monitoring, this study aimed to investigate the feasibility of optical imaging modalities—cross-polarization color (CPC) imaging, erythema index (EI) imaging, and laser speckle contrast (LSC) imaging—for continuous evaluation and monitoring of arthritis in animal models. C57BL/6 mice, used for the evaluation of arthritis, were divided into three groups: arthritic mice group (AMG), positive control mice group (PCMG), and negative control mice group (NCMG). Complete Freund’s adjuvant, mineral oil, and saline were injected into the footpad for AMG, PCMG, and NCMG, respectively. LSC and CPC images were acquired from 0 through 144 h after injection for all groups. EI images were calculated from CPC images. Variations in feet area, EI, and speckle index for each mice group over time were calculated for quantitative evaluation of arthritis. Histological examinations were performed, and the results were found to be consistent with those from optical imaging analysis. Thus, optical imaging modalities may be successfully applied for continuous evaluation and monitoring of arthritis in animal models.
authors.affiliation: Yonsei University, Department of Biomedical Engineering, 1 Yonseidae-gil, Wonju, Gangwon-do 220-710, Republic of Korea.
authors.forename: Taeyoon
authors.initials: T
authors.lastname: Son
authors.affiliation: Catholic University of Korea, Department of Internal Medicine, Research Institute of Immunobiology, 505 Banpo-dong, Seocho-gu, Seoul 137-701 Republic of Korea.
authors.forename: Hyung-Ju
authors.initials: HJ
authors.lastname: Yoon
authors.affiliation: Catholic University of Korea, Department of Internal Medicine, Research Institute of Immunobiology, 505 Banpo-dong, Seocho-gu, Seoul 137-701 Republic of Korea.
authors.forename: Saseong
authors.initials: S
authors.lastname: Lee
authors.affiliation: Yonsei University, Department of Radiological Science, 1 Yonseidae-gil, Wonju, Gangwon-do 220-710, Republic of Korea.
authors.forename: Won Seuk
authors.initials: WS
authors.lastname: Jang
authors.affiliation: Yonsei University, Department of Biomedical Engineering, 1 Yonseidae-gil, Wonju, Gangwon-do 220-710, Republic of Korea.
authors.forename: Byungjo
authors.initials: B
authors.lastname: Jung
authors.affiliation: Catholic University of Korea, Department of Internal Medicine, Research Institute of Immunobiology, 505 Banpo-dong, Seocho-gu, Seoul 137-701 Republic of KoreadCatholic University of Korea, Seoul St. Mary's Hospital, Department of Internal Medicine, Divisi.
authors.forename: Wan-Uk
authors.initials: WU
authors.lastname: Kim
mesh_terms: D000818:Animals; D001169:Arthritis, Experimental / Q000473:pathology*; D004890:Erythema / Q000473:pathology; D006614:Hindlimb / Q000473:pathology; D007091:Image Processing, Computer-Assisted; D007834:Lasers; D051379:Mice; D008810:Mice, Inbred C57BL; D061848:Optical Imaging / Q000379:methods*