title: Role of xenogenous bovine platelet gel embedded within collagen implant on tendon healing: an in vitro and in vivo study.
abstract: Surgical reconstruction of large Achilles tendon defects is demanding. Platelet concentrates may be useful to favor healing in such conditions. The characteristics of bovine platelet-gel embedded within a collagen-implant were determined in vitro, and its healing efficacy was examined in a large Achilles tendon defect in rabbits. Two cm of the left Achilles tendon of 60 rabbits were excised, and the animals were randomly assigned to control (no implant), collagen-implant, or bovine-platelet-gel-collagen-implant groups. The tendon edges were maintained aligned using a Kessler suture. No implant was inserted in the control group. In the two other groups, a collagen-implant or bovine-platelet-gel-collagen-implant was inserted in the defect. The bioelectricity and serum platelet-derived growth factor levels were measured weekly and at 60 days post injury, respectively. After euthanasia at 60 days post injury, the tendons were tested at macroscopic, microscopic, and ultrastructural levels, and their dry matter and biomechanical performances were also assessed. Another 60 rabbits were assigned to receive no implant, a collagen-implant, or a bovine-platelet-gel-collagen-implant, euthanized at 10, 20, 30, and 40 days post injury, and their tendons were evaluated grossly and histologically to determine host-graft interactions. Compared to the control and collagen-implant, treatment with bovine-platelet-gel-collagen-implant improved tissue bioelectricity and serum platelet-derived growth factor levels, and increased cell proliferation, differentiation, and maturation. It also increased number, diameter, and density of the collagen fibrils, alignment and maturation of the collagen fibrils and fibers, biomechanical properties and dry matter content of the injured tendons at 60 days post injury. The bovine-platelet-gel-collagen-implant also increased biodegradability, biocompatibility, and tissue incorporation behavior of the implant compared to the collagen-implant alone. This treatment also decreased tendon adhesion, muscle fibrosis, and atrophy, and improved the physical activity of the animals. The bovine-platelet-gel-collagen-implant was effective in neotenon formation in vivo, which may be valuable in the clinical setting.
authors.affiliation: Department of Pathology, School of Veterinary Medicine, Shiraz University, Shiraz, 71345, Iran.
authors.forename: Ahmad
authors.initials: A
authors.lastname: Oryan
authors.affiliation: Division of Surgery and Radiology, Department of Clinical Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, 71345, Iran dr.ali.moshiri@gmail.com.
authors.forename: Ali
authors.initials: A
authors.lastname: Moshiri
authors.affiliation: Division of Surgery and Radiology, Department of Clinical Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, 71345, Iran.
authors.forename: Abdolhamid
authors.initials: A
authors.lastname: Meimandi-Parizi
authors.affiliation: University of Salerno, Faculty of Medicine and Surgery, Department of Musculoskeletal Medicine and Surgery, and Centre for Sport and Exercise Medicine, Barts and the London School of Medicine and Dentistry, University of London, Queen Mary, E1 4DG, UK.
authors.forename: Nicola
authors.initials: N
authors.lastname: Maffulli
mesh_terms: D020341:Absorbable Implants; D000818:Animals; D001792:Blood Platelets; D002417:Cattle; D003094:Collagen; D036341:Intercellular Signaling Peptides and Proteins / Q000378:metabolism; D011817:Rabbits; D012038:Regeneration; D013708:Tendon Injuries / Q000378:metabolism* / Q000473:pathology / Q000628:therapy*; D013997:Time Factors
chemical_list: D036341:Intercellular Signaling Peptides and Proteins; D003094:Collagen
keywords: Tendon; biomechanics; collagen; host graft interaction; platelet gel; tissue engineering; ultrastructure