title: [Expression of nesfatin-1/NUCB2 and ghrelin in gastric mucosa of rats with intrauterine growth retardation].
abstract: OBJECTIVE
  To investigate the expression of nesfatin-1/NUCB2 and ghrelin in the gastric mucosa of rats with intrauterine growth retardation (IUGR) and its significance.
  
  
  METHODS
  The IUGR animal model was established by feeding rats low-protein diets during their pregnancy. Newborn rats were divided into catch-up growth, non-catch-up growth and control groups. Protein and mRNA levels of nesfatin-1/NUCB2 and ghrelin in the gastric mucosa of rats were determined by RT-PCR and Western blot, respectively.
  
  
  RESULTS
  Nesfatin-1/NUCB2 mRNA and protein were expressed in the gastric mucosa of rats immediately after birth, and their expression increased in an age-dependent manner in all three groups. Furthermore, the level of nesfatin-1/NUCB2 in the catch-up growth group was higher than that in the control group before weaning, whereas there was no significant difference in nesfatin-1/NUCB2 expression between the two groups after weaning. The level of nesfatin-1/NUCB2 in the non-catch-up growth group was lower than that in the catch-up growth group during the whole observation period. The level of ghrelin in the catch-up growth group was higher than that in the control group starting from day 12 after birth, whereas there was no significant difference in ghrelin expression between the two groups after weaning. The level of ghrelin in the non-catch-up growth group was lower compared with those in the catch-up growth and control groups from days 12 to 28 after birth.
  
  
  CONCLUSIONS
  Nesfatin-1 and ghrelin are co-expressed in the gastric mucosa of rats with IUGR after birth and interact with each other to produce long-term nutritional regulation.
authors.affiliation: Department of Pediatrics, Hebei General Hospital, Shijiazhuang 050051, China. sguangyao2@163.com.
authors.forename: Ya-Ying
authors.initials: YY
authors.lastname: Cheng
authors.forename: Hong-Yan
authors.initials: HY
authors.lastname: Lv
authors.forename: Xin
authors.initials: X
authors.lastname: Wang
authors.forename: Guang-Yao
authors.initials: GY
authors.lastname: Song
mesh_terms: D000367:Age Factors; D000818:Animals; D002135:Calcium-Binding Proteins / Q000032:analysis* / Q000235:genetics; D004268:DNA-Binding Proteins / Q000032:analysis* / Q000235:genetics; D005260:Female; D005317:Fetal Growth Retardation / Q000378:metabolism*; D005753:Gastric Mucosa / Q000737:chemistry*; D054439:Ghrelin / Q000032:analysis* / Q000235:genetics; D008297:Male; D009419:Nerve Tissue Proteins / Q000032:analysis* / Q000235:genetics; D000080866:Nucleobindins; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D002135:Calcium-Binding Proteins; D004268:DNA-Binding Proteins; D054439:Ghrelin; D009419:Nerve Tissue Proteins; C000630652:Nucb1 protein, rat; D000080866:Nucleobindins; D012333:RNA, Messenger