title: Ultrafast extraction of proteins from tissues using desorption by impulsive vibrational excitation.
abstract: A picosecond IR laser (PIRL) can be used to blast proteins out of tissues through desorption by impulsive excitation (DIVE) of intramolecular vibrational states of water molecules in the cell in less than a millisecond. With PIRL-DIVE proteins covering a range of a few kDa up to several MDa are extracted in high quantities compared to conventional approaches. The chemical composition of extracted proteins remains unaltered and even enzymatic activities are maintained.
authors.affiliation: University Medical Center Hamburg-Eppendorf, Department of Clinical Chemistry, Martinistrasse 52, Hamburg, 20246 (Germany).
authors.forename: Marcel
authors.initials: M
authors.lastname: Kwiatkowski
authors.forename: Marcus
authors.initials: M
authors.lastname: Wurlitzer
authors.forename: Maryam
authors.initials: M
authors.lastname: Omidi
authors.forename: Ling
authors.initials: L
authors.lastname: Ren
authors.forename: Sebastian
authors.initials: S
authors.lastname: Kruber
authors.forename: Refat
authors.initials: R
authors.lastname: Nimer
authors.forename: Wesley D
authors.initials: WD
authors.lastname: Robertson
authors.forename: Andrea
authors.initials: A
authors.lastname: Horst
authors.forename: R J Dwayne
authors.initials: RJ
authors.lastname: Miller
authors.forename: Hartmut
authors.initials: H
authors.lastname: Schlüter
mesh_terms: D000818:Animals; D007259:Infrared Rays; D007834:Lasers; D008099:Liver / Q000737:chemistry; D051379:Mice; D009132:Muscles / Q000737:chemistry; D011506:Proteins / Q000302:isolation & purification*; D040901:Proteomics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D011506:Proteins
keywords: mass spectrometry; picosecond IR laser; protein extraction; proteomics; tissue samples