title: Radiation-induced metabolomic changes in sterile male Μοnochamus alternatus (Coleoptera: Cerambycidae).
abstract: Radiation-induced sterile insect technique is a biologically based, environment-friendly method for the suppression or eradication of a number of insect pests. Although the basic mechanisms underlying the technology have been well studied, little is known about the cell responses in organisms. Characterization of the metabolic shift associated with radiation exposure in sterile insects would be helpful for understanding the detailed mechanism underlying this technique and promote its practical application. In this article, a metabolomic study was performed to characterize the global metabolic changes induced by radiation using untreated and 40 Gy (60)Coγ-irradiated testes of Japanese pine sawyer, Monochamus alternatus Hope. Differential metabolites were detected and tentatively identified. Many key metabolites in glycolysis and the tricarboxylic acid cycle, as well as most fatty and amino acids, were elevated in irradiated male M. alternatus, presumably resulting from depression of glycolysis and the tricarboxylic acid cycle, each of which are important pathways for energy generation Adenosine Triphosphate (ATP) in insect spermatozoa. The findings in this article will contribute to our knowledge of the characteristic metabolic changes associated with irradiation sterility and understand the molecular mechanisms underlying radiation-induced sterile insect technique.
authors.affiliation: Department of Entomology, China Agricultural University, Yuanmingyuan West Rd., Beijing 100193, China The Key Laboratory of Forest Protection, State Forestry Administration, Research Institute of Forest Ecology, Environment and Protection, Chinese Academy of Forestry, Beijing 100091, China.
authors.forename: L J
authors.initials: LJ
authors.lastname: Qu
authors.affiliation: State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China.
authors.forename: L J
authors.initials: LJ
authors.lastname: Wang
authors.affiliation: The Key Laboratory of Forest Protection, State Forestry Administration, Research Institute of Forest Ecology, Environment and Protection, Chinese Academy of Forestry, Beijing 100091, China.
authors.forename: Y A
authors.initials: YA
authors.lastname: Zhang
authors.affiliation: The Key Laboratory of Forest Protection, State Forestry Administration, Research Institute of Forest Ecology, Environment and Protection, Chinese Academy of Forestry, Beijing 100091, China.
authors.forename: Q H
authors.initials: QH
authors.lastname: Wang
authors.affiliation: The Key Laboratory of Forest Protection, State Forestry Administration, Research Institute of Forest Ecology, Environment and Protection, Chinese Academy of Forestry, Beijing 100091, China.
authors.forename: Y Z
authors.initials: YZ
authors.lastname: Wang
authors.affiliation: The Key Laboratory of Forest Protection, State Forestry Administration, Research Institute of Forest Ecology, Environment and Protection, Chinese Academy of Forestry, Beijing 100091, China.
authors.forename: T H
authors.initials: TH
authors.lastname: Zhao
authors.affiliation: Department of Entomology, China Agricultural University, Yuanmingyuan West Rd., Beijing 100193, China caiwz@cau.edu.cn.
authors.forename: W Z
authors.initials: WZ
authors.lastname: Cai
mesh_terms: D000818:Animals; D050260:Carbohydrate Metabolism / Q000528:radiation effects*; D001517:Coleoptera / Q000378:metabolism / Q000528:radiation effects*; D008401:Gas Chromatography-Mass Spectrometry; D007248:Infertility, Male; D050356:Lipid Metabolism / Q000528:radiation effects*; D008297:Male; D055442:Metabolome / Q000528:radiation effects*; D010572:Pest Control, Biological
keywords: gas chromatography time-of-flight mass spectrometry; metabolite; sterile insect technique; vector insect