title: Immunomodulatory Effects of ZYM-201 on LPS-stimulated B Cells.
abstract: ZYM-201 is a methyl ester of triterpenoid glycoside from Sanguisorba officinalis which has been used for treatment of inflammatory and metabolic diseases. In this study, immunomodulatory effects of ZYM-201 on B cells were examined in vitro and in vivo. When splenocytes were activated with lipopolysaccharide (LPS), the major population which had shown an increase in cell numbers was B cells. However, when the B cells were treated with ZYM-201 after LPS activation, their cell numbers and the expression of major costimulatory molecules, CD80 and CD86, were decreased. Furthermore, the effect of LPS, which induces activation of NF-κB, was abolished by ZYM-201: LPS-stimulated B cells showed decrease of phosphorylation after treatment of ZYM-201. The same results were shown in vivo experiments. These results suggest that ZYM-201 may play a role in the modulation of inflammatory responses through inhibiting NF-κB activation and downregulating the expression of costimulatory molecules on B cells.
authors.affiliation: Department of Bioinformatics and Life Science, Soongsil University, Seoul 156-743, Korea.
authors.forename: Ye Eun
authors.initials: YE
authors.lastname: Lee
authors.affiliation: Department of Bioinformatics and Life Science, Soongsil University, Seoul 156-743, Korea.
authors.forename: Soochan
authors.initials: S
authors.lastname: Kim
authors.affiliation: Department of Bioinformatics and Life Science, Soongsil University, Seoul 156-743, Korea.
authors.forename: Woong-Jae
authors.initials: WJ
authors.lastname: Jung
authors.affiliation: Department of Bioinformatics and Life Science, Soongsil University, Seoul 156-743, Korea.
authors.forename: Hyung Soo
authors.initials: HS
authors.lastname: Lee
authors.affiliation: Department of Bioinformatics and Life Science, Soongsil University, Seoul 156-743, Korea.
authors.forename: Mi-Yeon
authors.initials: MY
authors.lastname: Kim
keywords: B cell; Inflammation; LPS; ZYM-201