title: The thermosensitive TRPV3 channel contributes to rapid wound healing in oral epithelia.
abstract: The oral cavity provides an entrance to the alimentary tract to serve as a protective barrier against harmful environmental stimuli. The oral mucosa is susceptible to injury because of its location; nonetheless, it has faster wound healing than the skin and less scar formation. However, the molecular pathways regulating this wound healing are unclear. Here, we show that transient receptor potential vanilloid 3 (TRPV3), a thermosensitive Ca(2+)-permeable channel, is more highly expressed in murine oral epithelia than in the skin by quantitative RT-PCR. We found that temperatures above 33°C activated TRPV3 and promoted oral epithelial cell proliferation. The proliferation rate in the oral epithelia of TRPV3 knockout (TRPV3KO) mice was less than that of wild-type (WT) mice. We investigated the contribution of TRPV3 to wound healing using a molar tooth extraction model and found that oral wound closure was delayed in TRPV3KO mice compared with that in WT mice. TRPV3 mRNA was up-regulated in wounded tissues, suggesting that TRPV3 may contribute to oral wound repair. We identified TRPV3 as an essential receptor in heat-induced oral epithelia proliferation and wound healing. Our findings suggest that TRPV3 activation could be a potential therapeutic target for wound healing in skin and oral mucosa.
authors.affiliation: Department of Molecular Cell Biology and Oral Anatomy, Graduate School of Dental Science, Kyushu University, Fukuoka, Japan; Department of Oral and Maxillofacial Surgery, Faculty of Medicine, Saga University, Saga, Japan; Division of Histology and Neuroanatomy, Department of Anatomy and Physiology, Faculty of Medicine, Saga University, Saga, Japan;
authors.forename: Reona
authors.initials: R
authors.lastname: Aijima
authors.affiliation: Department of Molecular Cell Biology and Oral Anatomy, Graduate School of Dental Science, Kyushu University, Fukuoka, Japan;
authors.forename: Bing
authors.initials: B
authors.lastname: Wang
authors.affiliation: Department of Molecular Cell Biology and Oral Anatomy, Graduate School of Dental Science, Kyushu University, Fukuoka, Japan;
authors.forename: Tomoka
authors.initials: T
authors.lastname: Takao
authors.affiliation: Division of Cell Signaling, Okazaki Institute for Integrative Bioscience (National Institute for Physiological Sciences), National Institutes of Natural Sciences, Okazaki, Japan; and.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Mihara
authors.affiliation: Division of Cell Signaling, Okazaki Institute for Integrative Bioscience (National Institute for Physiological Sciences), National Institutes of Natural Sciences, Okazaki, Japan; and.
authors.forename: Makiko
authors.initials: M
authors.lastname: Kashio
authors.affiliation: Department of Molecular Cell Biology and Oral Anatomy, Graduate School of Dental Science, Kyushu University, Fukuoka, Japan;
authors.forename: Yasuyoshi
authors.initials: Y
authors.lastname: Ohsaki
authors.affiliation: Department of Molecular Cell Biology and Oral Anatomy, Graduate School of Dental Science, Kyushu University, Fukuoka, Japan;
authors.forename: Jing-Qi
authors.initials: JQ
authors.lastname: Zhang
authors.affiliation: Department of Pharmacology, Jichi Medical University, Shimotsuke, Japan.
authors.forename: Atsuko
authors.initials: A
authors.lastname: Mizuno
authors.affiliation: Department of Pharmacology, Jichi Medical University, Shimotsuke, Japan.
authors.forename: Makoto
authors.initials: M
authors.lastname: Suzuki
authors.affiliation: Department of Oral and Maxillofacial Surgery, Faculty of Medicine, Saga University, Saga, Japan;
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Yamashita
authors.affiliation: Division of Histology and Neuroanatomy, Department of Anatomy and Physiology, Faculty of Medicine, Saga University, Saga, Japan;
authors.forename: Sadahiko
authors.initials: S
authors.lastname: Masuko
authors.affiliation: Department of Oral and Maxillofacial Surgery, Faculty of Medicine, Saga University, Saga, Japan;
authors.forename: Masaaki
authors.initials: M
authors.lastname: Goto
authors.affiliation: Division of Cell Signaling, Okazaki Institute for Integrative Bioscience (National Institute for Physiological Sciences), National Institutes of Natural Sciences, Okazaki, Japan; and.
authors.forename: Makoto
authors.initials: M
authors.lastname: Tominaga
authors.affiliation: Department of Molecular Cell Biology and Oral Anatomy, Graduate School of Dental Science, Kyushu University, Fukuoka, Japan; kido@dent.kyushu-u.ac.jp.
authors.forename: Mizuho A
authors.initials: MA
authors.lastname: Kido
mesh_terms: D000818:Animals; D049109:Cell Proliferation; D002478:Cells, Cultured; D004195:Disease Models, Animal; D066246:ErbB Receptors / Q000378:metabolism; D006358:Hot Temperature; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009061:Mouth Mucosa / Q000293:injuries* / Q000473:pathology / Q000503:physiopathology; D010766:Phosphorylation; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D050916:TRPV Cation Channels / Q000172:deficiency / Q000235:genetics / Q000502:physiology*; D014081:Tooth Extraction; D014945:Wound Healing / Q000235:genetics / Q000502:physiology*
chemical_list: D012333:RNA, Messenger; D050916:TRPV Cation Channels; C486760:Trpv3 protein, mouse; C541485:EGFR protein, mouse; D066246:ErbB Receptors
keywords: ambient temperature; oral mucosa; wound repair