title: Podosome-regulating kinesin KIF1C translocates to the cell periphery in a CLASP-dependent manner.
abstract: The kinesin KIF1C is known to regulate podosomes, actin-rich adhesion structures that remodel the extracellular matrix during physiological processes. Here, we show that KIF1C is a player in the podosome-inducing signaling cascade. Upon induction of podosome formation by protein kinase C (PKC), KIF1C translocation to the cell periphery intensifies and KIF1C accumulates both in the proximity of peripheral microtubules that show enrichment for the plus-tip-associated proteins CLASPs and around podosomes. Importantly, without CLASPs, both KIF1C trafficking and podosome formation are suppressed. Moreover, chimeric mitochondrially targeted CLASP2 recruits KIF1C, suggesting a transient CLASP-KIF1C association. We propose that CLASPs create preferred microtubule tracks for KIF1C to promote podosome induction downstream of PKC.
authors.affiliation: Department of Cell and Developmental Biology, Vanderbilt University Medical Center, Nashville 37232, TN, USA.
authors.forename: Nadia
authors.initials: N
authors.lastname: Efimova
authors.affiliation: Department of Cell and Developmental Biology, Vanderbilt University Medical Center, Nashville 37232, TN, USA.
authors.forename: Ashley
authors.initials: A
authors.lastname: Grimaldi
authors.affiliation: Centre for Mechanochemical Cell Biology, Warwick Medical School, University of Warwick, Coventry CV4 7AL, UK.
authors.forename: Alice
authors.initials: A
authors.lastname: Bachmann
authors.affiliation: Department of Cell and Developmental Biology, Vanderbilt University Medical Center, Nashville 37232, TN, USA.
authors.forename: Keyada
authors.initials: K
authors.lastname: Frye
authors.affiliation: Department of Cell and Developmental Biology, Vanderbilt University Medical Center, Nashville 37232, TN, USA.
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Zhu
authors.affiliation: Department of Cell and Developmental Biology, Vanderbilt University Medical Center, Nashville 37232, TN, USA.
authors.forename: Alexander
authors.initials: A
authors.lastname: Feoktistov
authors.affiliation: Centre for Mechanochemical Cell Biology, Warwick Medical School, University of Warwick, Coventry CV4 7AL, UK.
authors.forename: Anne
authors.initials: A
authors.lastname: Straube
authors.affiliation: Department of Cell and Developmental Biology, Vanderbilt University Medical Center, Nashville 37232, TN, USA irina.kaverina@vanderbilt.edu.
authors.forename: Irina
authors.initials: I
authors.lastname: Kaverina
mesh_terms: D000818:Animals; D002460:Cell Line; D022081:Cell Surface Extensions / Q000378:metabolism*; D006801:Humans; D016547:Kinesins / Q000378:metabolism*; D008869:Microtubule-Associated Proteins / Q000378:metabolism*; D008870:Microtubules / Q000378:metabolism; D008954:Models, Biological; D009131:Muscle, Smooth, Vascular / Q000166:cytology; D032389:Myocytes, Smooth Muscle / Q000166:cytology / Q000378:metabolism; D011493:Protein Kinase C / Q000378:metabolism; D021381:Protein Transport; D051381:Rats; D015398:Signal Transduction
chemical_list: C000602629:CLASP1 protein, rat; C577861:CLASP2 protein, rat; C114063:KIF1C protein, human; D008869:Microtubule-Associated Proteins; D011493:Protein Kinase C; C000602628:KIF1C protein, rat; D016547:Kinesins
keywords: CLASP; KIF1C; Kinesin; Microtubule; Podosome; Trafficking