title: TMIO-PyrImid hybrids are profluorescent, site-directed spin labels for nucleic acids.
abstract: We report the synthesis of a new class of molecules which are hybrids of long-lived tetramethylisoindolinoxyl (TMIO) radicals and the pyrido[1,2-a]benzimidazole (PyrImid) scaffold. These compounds represent a new lead for noncovalently binding nucleic acid probes, as they interact with nucleic acids with previously unreported C (DNA) and C/U (RNA) complementarity, which can be detected by electron paramagnetic resonance (EPR) techniques. They also have promising properties for fluorimetric analysis, as their fluorescent spin-quenched derivatives exhibit a significant Stokes shift.
authors.affiliation: Faculty of Science and Engineering, Queensland University of Technology , P.O. Box 2434, 2 George Street, Brisbane, QLD 4001, Australia.
authors.forename: Benjamin A
authors.initials: BA
authors.lastname: Chalmers
authors.forename: Subham
authors.initials: S
authors.lastname: Saha
authors.forename: Tri
authors.initials: T
authors.lastname: Nguyen
authors.forename: John
authors.initials: J
authors.lastname: McMurtrie
authors.forename: Snorri Th
authors.initials: ST
authors.lastname: Sigurdsson
authors.forename: Steven E
authors.initials: SE
authors.lastname: Bottle
authors.forename: Kye-Simeon
authors.initials: KS
authors.lastname: Masters
mesh_terms: D001562:Benzimidazoles / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D004578:Electron Spin Resonance Spectroscopy / Q000379:methods*; D054833:Isoindoles / Q000737:chemistry*; D015394:Molecular Structure; D009696:Nucleic Acids / Q000737:chemistry*; D011725:Pyridines / Q000737:chemistry*; D012313:RNA / Q000737:chemistry*; D013113:Spin Labels / Q000138:chemical synthesis*
chemical_list: D001562:Benzimidazoles; D054833:Isoindoles; D009696:Nucleic Acids; D011725:Pyridines; D013113:Spin Labels; D012313:RNA; D004247:DNA