title: SUMO2/3 is associated with ubiquitinated protein aggregates in the mouse neocortex after middle cerebral artery occlusion.
abstract: Protein modifications cooperatively act to protect the proteome from cellular stress. Focal cerebral ischemia increases protein ubiquitination, resulting in formation of ubiquitin-rich aggregates. A concurrent elevation in small ubiquitin-related modifier (SUMO)-conjugated proteins has also been reported, but a potential connection to ubiquitin remains unexplored. Here we show that SUMO2/3 conjugates are present in postischemic ubiquitin-rich aggregates, physically associated with ubiquitin. The coaggregation of SUMO2/3 and ubiquitin is induced rapidly after ischemia, depends on reperfusion, and is also observed in the absence of ischemic damage. The association between SUMO and ubiquitin suggests overlapping functional roles after ischemia/reperfusion.
authors.affiliation: Feil Family Brain and Mind Research Institute, Weill Cornell Medical College, New York, NY, USA.
authors.forename: Karin
authors.initials: K
authors.lastname: Hochrainer
authors.affiliation: Feil Family Brain and Mind Research Institute, Weill Cornell Medical College, New York, NY, USA.
authors.forename: Katherine
authors.initials: K
authors.lastname: Jackman
authors.affiliation: Feil Family Brain and Mind Research Institute, Weill Cornell Medical College, New York, NY, USA.
authors.forename: Corinne
authors.initials: C
authors.lastname: Benakis
authors.affiliation: Feil Family Brain and Mind Research Institute, Weill Cornell Medical College, New York, NY, USA.
authors.forename: Josef
authors.initials: J
authors.lastname: Anrather
authors.affiliation: Feil Family Brain and Mind Research Institute, Weill Cornell Medical College, New York, NY, USA.
authors.forename: Costantino
authors.initials: C
authors.lastname: Iadecola
mesh_terms: D000818:Animals; D015153:Blotting, Western; D004195:Disease Models, Animal; D004591:Electrophoresis, Polyacrylamide Gel; D047468:Immunoprecipitation; D020244:Infarction, Middle Cerebral Artery / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008810:Mice, Inbred C57BL; D019579:Neocortex / Q000378:metabolism* / Q000473:pathology; D066329:Protein Aggregates; D015427:Reperfusion Injury / Q000378:metabolism; D025842:SUMO-1 Protein / Q000378:metabolism*; D025841:Small Ubiquitin-Related Modifier Proteins / Q000378:metabolism*; D058207:Sumoylation; D025801:Ubiquitin / Q000378:metabolism*
chemical_list: D066329:Protein Aggregates; D025842:SUMO-1 Protein; C515972:SUMO2 protein, mouse; D025841:Small Ubiquitin-Related Modifier Proteins; D025801:Ubiquitin