title: Comparative analyses of nonpathogenic, opportunistic, and totally pathogenic mycobacteria reveal genomic and biochemical variabilities and highlight the survival attributes of Mycobacterium tuberculosis.
abstract: Mycobacterial evolution involves various processes, such as genome reduction, gene cooption, and critical gene acquisition. Our comparative genome size analysis of 44 mycobacterial genomes revealed that the nonpathogenic (NP) genomes were bigger than those of opportunistic (OP) or totally pathogenic (TP) mycobacteria, with the TP genomes being smaller yet variable in size--their genomic plasticity reflected their ability to evolve and survive under various environmental conditions. From the 44 mycobacterial species, 13 species, representing TP, OP, and NP, were selected for genomic-relatedness analyses. Analysis of homologous protein-coding genes shared between Mycobacterium indicus pranii (NP), Mycobacterium intracellulare ATCC 13950 (OP), and Mycobacterium tuberculosis H37Rv (TP) revealed that 4,995 (i.e., ~95%) M. indicaus pranii proteins have homology with M. intracellulare, whereas the homologies among M. indicus pranii, M. intracellulare ATCC 13950, and M. tuberculosis H37Rv were significantly lower. A total of 4,153 (~79%) M. indicus pranii proteins and 4,093 (~79%) M. intracellulare ATCC 13950 proteins exhibited homology with the M. tuberculosis H37Rv proteome, while 3,301 (~82%) and 3,295 (~82%) M. tuberculosis H37Rv proteins showed homology with M. indicus pranii and M. intracellulare ATCC 13950 proteomes, respectively. Comparative metabolic pathway analyses of TP/OP/NP mycobacteria showed enzymatic plasticity between M. indicus pranii (NP) and M. intracellulare ATCC 13950 (OP), Mycobacterium avium 104 (OP), and M. tuberculosis H37Rv (TP). Mycobacterium tuberculosis seems to have acquired novel alternate pathways with possible roles in metabolism, host-pathogen interactions, virulence, and intracellular survival, and by implication some of these could be potential drug targets.
  
  
  OBJECTIVE
  The complete sequence analysis of Mycobacterium indicus pranii, a novel species of Mycobacterium shown earlier to have strong immunomodulatory properties and currently in use for the treatment of leprosy, places it evolutionarily at the point of transition to pathogenicity. With the purpose of establishing the importance of M. indicus pranii in providing insight into the virulence mechanism of tuberculous and nontuberculous mycobacteria, we carried out comparative genomic and proteomic analyses of 44 mycobacterial species representing nonpathogenic (NP), opportunistic (OP), and totally pathogenic (TP) mycobacteria. Our results clearly placed M. indicus pranii as an ancestor of the M. avium complex. Analyses of comparative metabolic pathways between M. indicus pranii (NP), M. tuberculosis (TP), and M. intracellulare (OP) pointed to the presence of novel alternative pathways in M. tuberculosis with implications for pathogenesis and survival in the human host and identification of new drug targets.
authors.affiliation: European Molecular Biology Laboratory, European Bioinformatics Institute, Wellcome Trust Genome Campus, Hinxton, United Kingdom asad@ebi.ac.uk seyedhasnain@gmail.com.
authors.forename: Syed Asad
authors.initials: SA
authors.lastname: Rahman
authors.affiliation: Kusuma School of Biological Sciences, Indian Institute of Technology, Hauz Khas, New Delhi, India.
authors.forename: Yadvir
authors.initials: Y
authors.lastname: Singh
authors.affiliation: Kusuma School of Biological Sciences, Indian Institute of Technology, Hauz Khas, New Delhi, India.
authors.forename: Sakshi
authors.initials: S
authors.lastname: Kohli
authors.affiliation: Department of Biophysics, All India Institute of Medical Sciences, New Delhi, India.
authors.forename: Javeed
authors.initials: J
authors.lastname: Ahmad
authors.forename: Nasreen Z
authors.initials: NZ
authors.lastname: Ehtesham
authors.affiliation: Department of Biochemistry, University of Delhi South Campus, New Delhi, India.
authors.forename: Anil K
authors.initials: AK
authors.lastname: Tyagi
authors.affiliation: asad@ebi.ac.uk seyedhasnain@gmail.com.
authors.forename: Seyed E
authors.initials: SE
authors.lastname: Hasnain
mesh_terms: D000220:Adaptation, Biological; D000222:Adaptation, Physiological; D001426:Bacterial Proteins / Q000235:genetics; D016000:Cluster Analysis; D004783:Environmental Microbiology; D019143:Evolution, Molecular; D014644:Genetic Variation; D016680:Genome, Bacterial; D006801:Humans; D053858:Metabolic Networks and Pathways / Q000235:genetics*; D009161:Mycobacterium / Q000235:genetics* / Q000378:metabolism / Q000472:pathogenicity; D010802:Phylogeny; D017386:Sequence Homology, Amino Acid; D014376:Tuberculosis / Q000382:microbiology*
chemical_list: D001426:Bacterial Proteins