title: Electrogenerated chemiluminescence resonance energy transfer between luminol and CdSe@ZnS quantum dots and its sensing application in the determination of thrombin.
abstract: In this work, electrogenerated chemiluminescence resonance energy transfer (ECL-RET) between luminol as a donor and CdSe@ZnS quantum dots (QDs) as an acceptor was reported in neutral conditions. It was observed that a glassy carbon electrode modified with CdSe@ZnS quantum dots (CdSe@ZnS/GCE) can catalyze the luminol oxidation to promote the anodic luminol ECL without coreactants. The intensity of anodic luminol ECL (0.60 V) at the CdSe@ZnS/GCE was enhanced more than 1 order of magnitude compared with that at the bare GCE. Another stronger anodic ECL peak observed at more positive potential (1.10 V) could be assigned to the ECL-RET between the excited state of luminol and the QDs. A label-free ECL aptasensor for the detection of thrombin was fabricated based on the synergic effect of the electrocatalysis and the ECL-RET. The approach showed high sensitivity, good selectivity, and wide linearity for the detection of thrombin in the range of 10 fM-100 pM with a detection limit of 1.4 fM (S/N = 3). The results suggested that the as-proposed luminol-QDs ECL biosensor will be promising in the detection of protein.
authors.affiliation: State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University , Nanjing 210093, China.
authors.forename: Yong-Ping
authors.initials: YP
authors.lastname: Dong
authors.forename: Ting-Ting
authors.initials: TT
authors.lastname: Gao
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhou
authors.forename: Jun-Jie
authors.initials: JJ
authors.lastname: Zhu
mesh_terms: D019187:Cadmium Compounds / Q000138:chemical synthesis / Q000737:chemistry*; D004735:Energy Transfer; D008163:Luminescent Measurements; D008165:Luminol / Q000737:chemistry*; D045663:Quantum Dots; D018036:Selenium Compounds / Q000138:chemical synthesis / Q000737:chemistry*; D013440:Sulfides / Q000138:chemical synthesis / Q000737:chemistry*; D013917:Thrombin / Q000032:analysis*; D017967:Zinc Compounds / Q000138:chemical synthesis / Q000737:chemistry*
chemical_list: D019187:Cadmium Compounds; D018036:Selenium Compounds; D013440:Sulfides; D017967:Zinc Compounds; D008165:Luminol; C058667:cadmium selenide; D013917:Thrombin; C031238:zinc sulfide