title: Protective effect of ultrafiltered XinMaiJia extract against H₂O₂-induced injury in human umbilical vein endothelial cells through NHE1 downregulation.
abstract: We examined the protective effects of ultrafiltered XinMaiJia (XMJ) extract in a hydrogen peroxide (H2O2)-induced injury model in human umbilical vein endothelial cells (HUVECs) and determined the corresponding changes in the Na(+)-H(+) exchanger (NHE1) protein content and NHE1 gene expression. H2O2-induced HUVECs were treated with different concentrations of XMJ extract and the corresponding changes in morphology, activity, membrane permeability, biochemical indicators, cytokine concentration, NHE1 protein content, and NHE1 gene expression were determined. H2O2 significantly promoted HUVEC injury, whereas ultrafiltered XMJ extract significantly improved the morphological changes in injured HUVECs, increased their activity, and decreased NHE1 gene and protein expression, as well as limited the decrease in membrane permeability and expression of intercellular adhesion molecule-1, vascular cell adhesion molecule-1, interleukin (IL)-1, IL-6, and nuclear factor-kB. Ultrafiltered XMJ extract inhibited H2O2-induced HUVEC injury by inhibiting NHE1 activity.
authors.affiliation: School of Basic Medical Sciences, Xinxiang Medical University, Xinxiang, China.
authors.forename: Y L
authors.initials: YL
authors.lastname: Yin
authors.affiliation: College of Pharmacy, Xinxiang Medical University, Xinxiang, China.
authors.forename: P
authors.initials: P
authors.lastname: Li
authors.affiliation: Puyang City Health School, Puyang, China.
authors.forename: J
authors.initials: J
authors.lastname: Yang
authors.affiliation: Department of Nephrology, The First Affiliated Hospital, Zhengzhou University, Zhengzhou, China.
authors.forename: D W
authors.initials: DW
authors.lastname: Liu
authors.affiliation: Department of Inspection, Xinxiang Medical University, Xinxiang, China.
authors.forename: R L
authors.initials: RL
authors.lastname: Sun
authors.affiliation: College of Pharmacy, Xinxiang Medical University, Xinxiang, China.
authors.forename: G P
authors.initials: GP
authors.lastname: Pan
authors.affiliation: Ophthalmology, The First Affiliated Hospital, Zhengzhou University, Zhengzhou, China.
authors.forename: G M
authors.initials: GM
authors.lastname: Wan
authors.affiliation: College of Pharmacy, Xinxiang Medical University, Xinxiang, China guangruiwan@126.com.
authors.forename: G R
authors.initials: GR
authors.lastname: Wan
mesh_terms: D027682:Cation Transport Proteins / Q000235:genetics / Q000378:metabolism*; D002463:Cell Membrane Permeability / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D016207:Cytokines / Q000096:biosynthesis; D004305:Dose-Response Relationship, Drug; D015536:Down-Regulation; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D015870:Gene Expression; D061307:Human Umbilical Vein Endothelial Cells / Q000187:drug effects* / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D006861:Hydrogen Peroxide / Q000009:adverse effects*; D020011:Protective Agents / Q000494:pharmacology*; D000074082:Sodium-Hydrogen Exchanger 1; D017923:Sodium-Hydrogen Exchangers / Q000235:genetics / Q000378:metabolism*; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D027682:Cation Transport Proteins; D016207:Cytokines; D004365:Drugs, Chinese Herbal; D020011:Protective Agents; C490998:SLC9A1 protein, human; D000074082:Sodium-Hydrogen Exchanger 1; D017923:Sodium-Hydrogen Exchangers; D006861:Hydrogen Peroxide; D013482:Superoxide Dismutase