title: Protective effects of resveratrol oligomers from Vitis amurensis against sodium nitroprusside-induced neurotoxicity in human neuroblastoma SH-SY5Y cells.
abstract: Nitric oxide (NO) induces apoptosis in neuronal cells, and has been implicating in a variety of neuronal pathological process. Thus, there is much interest in identifying natural substances which have protective effects against damage induced by nitrosative stress. The roots of Vitis amurensis have been used as traditional medicine and contain structurally diverse resveratrol oligomers with various biological activities. However, there have been few studies on the protective effect of resveratrol oligomers against neurotoxic reactive nitrogen species. In this study, we evaluated the protective effects of two resveratrol oligomers from V. amurensis, vitisin A and heyneanol A, against NO-induced toxicity. Additionally, their antioxidant activities were determined by measuring NO and hydroxyl radical scavenging ability. Both vitisin A and heyneanol A reduced cell death and DNA fragmentation induced by sodium nitroprusside in SH-SY5Y cells. The present study indicates that radical scavenging activities of vitisin A and heyneanol A contribute to protecting neuronal cells against nitrosative stress.
authors.affiliation: National Forensic Service, 331-1 Sinwol-7-dong Yangcheon-gu, Seoul, 158-707, Republic of Korea.
authors.forename: Moonhee
authors.initials: M
authors.lastname: Jang
authors.forename: Eun Jung
authors.initials: EJ
authors.lastname: Cho
authors.forename: Xiang-Lan
authors.initials: XL
authors.lastname: Piao
mesh_terms: D000975:Antioxidants / Q000494:pharmacology; D001572:Benzofurans / Q000494:pharmacology; D002460:Cell Line; D053938:DNA Fragmentation / Q000187:drug effects; D016166:Free Radical Scavengers / Q000494:pharmacology; D006801:Humans; D017665:Hydroxyl Radical / Q000378:metabolism; D018696:Neuroprotective Agents / Q000627:therapeutic use*; D020258:Neurotoxicity Syndromes / Q000517:prevention & control*; D009569:Nitric Oxide / Q000037:antagonists & inhibitors / Q000633:toxicity; D009599:Nitroprusside / Q000633:toxicity*; D010636:Phenols / Q000494:pharmacology; D018517:Plant Roots / Q000737:chemistry; D026361:Reactive Nitrogen Species / Q000378:metabolism; D000077185:Resveratrol; D013267:Stilbenes / Q000494:pharmacology / Q000627:therapeutic use*; D027843:Vitis / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D001572:Benzofurans; D016166:Free Radical Scavengers; D018696:Neuroprotective Agents; D010636:Phenols; D026361:Reactive Nitrogen Species; D013267:Stilbenes; C482862:heyneanol A; C412469:vitisin A; D009599:Nitroprusside; D009569:Nitric Oxide; D017665:Hydroxyl Radical; D000077185:Resveratrol