title: Transactivation of large ribozymes.
abstract: The domains of large ribozymes are composed of secondary structural elements that fold independently in solution. Interactions between these structures contribute to the overall structure of the ribozyme. These elements can also be deleted, reducing catalytic activity, and their structure-function relationships determined by assaying the ability of variant elements to activate the deleted ribozyme in trans. This is illustrated for an unusual loop structure from Bacillus subtilis Ribonuclease P RNA.
authors.affiliation: Department of Biochemistry, University of Missouri, 117 Schweitzer Hall, 103A Schlundt Annex, Columbia, MO, 65211, USA.
authors.forename: Matthew B
authors.initials: MB
authors.lastname: Martin
authors.forename: Thomas L
authors.initials: TL
authors.lastname: Leeper
authors.forename: Frank J
authors.initials: FJ
authors.lastname: Schmidt
mesh_terms: D001412:Bacillus subtilis / Q000201:enzymology; D001483:Base Sequence; D008967:Molecular Biology / Q000379:methods*; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics; D016337:RNA, Catalytic / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D043262:Ribonuclease P / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D015533:Transcriptional Activation / Q000235:genetics*
chemical_list: D016337:RNA, Catalytic; D043262:Ribonuclease P