title: SIRT2 plays a key role in both cell cycle regulation and cell survival of BV2 microglia.
abstract: Sirtuin 2 (SIRT2) is a member of sirtuin protein family. Previous studies have suggested that SIRT2 plays differential roles in the survival and cell cycle regulation of various cell types. Because microglia plays critical roles in multiple major neurological disorders, in our current study we investigated the roles of SIRT2 in regulation of the cell cycle and cell survival of BV2 microglia by applying SIRT2 siRNA. We found that SIRT2 reductions by SIRT2 siRNA can produce cell cycle arrest of the cells at G0/G1 phase, by significantly increasing percentage of the cells in G0/G1 phase as well as decreasing percentage of the cells in S phase. The SIRT2 reductions can also increase late-stage apoptosis of the cells. We further found that SIRT2 silencing can lead to a decrease in the number of surviving BV2 cells, which may result from the effects of SIRT2 siRNA on both cell cycle and cell survival of the cells. Collectively, our study has suggested an important role of SIRT2 in regulating both the cell cycle and basal survival of microglia.
authors.affiliation: Med-X Research Institute and School of Biomedical Engineering, Shanghai Jiao Tong University Shanghai 200030, PR China.
authors.forename: Hui
authors.initials: H
authors.lastname: Nie
authors.affiliation: Med-X Research Institute and School of Biomedical Engineering, Shanghai Jiao Tong University Shanghai 200030, PR China ; Microbiology Institute of Shanxi Xi'an 710043, PR China.
authors.forename: Yexin
authors.initials: Y
authors.lastname: Li
authors.affiliation: Med-X Research Institute and School of Biomedical Engineering, Shanghai Jiao Tong University Shanghai 200030, PR China.
authors.forename: Caixia
authors.initials: C
authors.lastname: Wang
authors.affiliation: Shanghai Key Laboratory of Gastric Neoplasms, Department of Surgery, Shanghai Institute of Digestive Surgery, Rui Jin Hospital, Shanghai Jiao Tong University School of Medicine Shanghai 200025, PR China.
authors.forename: Xuehua
authors.initials: X
authors.lastname: Chen
authors.affiliation: Shanghai Key Laboratory of Gastric Neoplasms, Department of Surgery, Shanghai Institute of Digestive Surgery, Rui Jin Hospital, Shanghai Jiao Tong University School of Medicine Shanghai 200025, PR China.
authors.forename: Bingya
authors.initials: B
authors.lastname: Liu
authors.affiliation: Department of Neurology, Shanghai Third People's Hospital, Shanghai Jiao Tong University School of Medicine Shanghai 201999, PR China.
authors.forename: Danhong
authors.initials: D
authors.lastname: Wu
authors.affiliation: Med-X Research Institute and School of Biomedical Engineering, Shanghai Jiao Tong University Shanghai 200030, PR China ; Department of Neurology, Rui Jin Hospital, Shanghai Jiao Tong University School of Medicine Shanghai 200025, PR China.
authors.forename: Weihai
authors.initials: W
authors.lastname: Ying
keywords: SIRT2; apoptosis; cell cycle; microglia; neurological disorders