title: Identification of antisense RNA stem-loops that inhibit RNA-protein interactions using a bacterial reporter system.
abstract: RNA-protein interactions play important roles in gene regulation, functional RNA-protein complexes such as the ribosome, and in viral replication. Molecules that regulate specific RNA-protein interactions may be used to dissect biological processes, and to establish the validity of targeting an RNA-protein interaction. There are many examples of biological regulation by antisense RNA stem-loops that form loop-loop and loop-linear RNA-RNA interactions. Here, a bacterial reporter system for the identification of RNA stem-loops that inhibit the formation of RNA-protein complexes through RNA-RNA interactions is described.
authors.affiliation: Department of Life Sciences, Tokyo Gakugei University, 4-1-1 Nukuikita-machi, Koganei, Tokyo, 184-8501, Japan, harada@u-gakugei.ac.jp.
authors.forename: Kazuo
authors.initials: K
authors.lastname: Harada
mesh_terms: D001483:Base Sequence; D003114:Colony-Forming Units Assay; D004926:Escherichia coli / Q000378:metabolism*; D017930:Genes, Reporter; D008967:Molecular Biology / Q000379:methods*; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D010455:Peptides / Q000378:metabolism; D010957:Plasmids / Q000378:metabolism; D011485:Protein Binding; D016372:RNA, Antisense / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000378:metabolism*; D001616:beta-Galactosidase / Q000378:metabolism
chemical_list: D010455:Peptides; D016372:RNA, Antisense; D016601:RNA-Binding Proteins; D001616:beta-Galactosidase