title: Analysis of imprecision in incurred sample reanalysis for small molecules.
abstract: Over the years, incurred sample (IS) reanalysis (ISR) has become a tool to confirm the reliability of bioanalytical measurements. The recommendation for ISR acceptance criterion for small molecules is at least 67% of ISR samples that have reanalyzed concentrations within 20% of their original concentrations when normalized to their means. To understand the relevance of the ISR acceptance criterion and sample size requirements, simulated ISR studies evaluated the probability of ISR studies passing the acceptance criterion (ISR pass rate) as a function of IS imprecision and sample size. When IS imprecision (percent coefficient of variation: %CV) is low (≤ 10 or 1-10% CV), high ISR pass rate (≥ 99%) is attained with <50 samples. At intermediate IS imprecision (e.g., 12% CV or 7-12% CV range), 80-160 samples are required for a high ISR pass rate. When IS imprecision is at the higher end of the acceptance limit, ISR pass rate decreases significantly, and increasing sample size fails to achieve high ISR pass rate. The effect of systematic bias (e.g., instability, interconversion) on ISR pass rate is strongly dependent on sample size at intermediate IS imprecision. The results provide an understanding of the effect of IS imprecision on ISR pass rates and a framework for selection of ISR sample sizes.
authors.affiliation: Division of Bioequivalence III, Office of Bioequivalence, Office of Generic Drugs, Food and Drug Administration, 10903 New Hampshire Avenue, Silver Spring, Maryland, 20993, USA, sriram.subramaniam@fda.hhs.gov.
authors.forename: Sriram
authors.initials: S
authors.lastname: Subramaniam
authors.forename: Devvrat
authors.initials: D
authors.lastname: Patel
authors.forename: Barbara M
authors.initials: BM
authors.lastname: Davit
authors.forename: Dale P
authors.initials: DP
authors.lastname: Conner
mesh_terms: D002623:Chemistry Techniques, Analytical / Q000379:methods*; D003198:Computer Simulation; D004364:Pharmaceutical Preparations / Q000032:analysis*; D011786:Quality Control; D015203:Reproducibility of Results; D018401:Sample Size
chemical_list: D004364:Pharmaceutical Preparations