title: Histamine-releasing factor/translationally controlled tumor protein plays a role in induced cell adhesion, apoptosis resistance and chemoresistance in non-Hodgkin lymphomas.
abstract: Mounting evidence has proved that cellular adhesion confers resistance to chemotherapy in multiple lymphomas. The molecular mechanism underlying cell adhesion-mediated drug resistance (CAM-DR) is, however, poorly understood. In this study, we investigated the expression and biologic function of histamine-releasing factor (HRF) in non-Hodgkin lymphomas (NHLs). Clinically, by immunohistochemistry analysis we observed obvious up-regulation of HRF in NHLs including diffuse large B-cell lymphoma (DLBCL), follicular lymphoma (FL) and natural killer (NK)/T-cell lymphoma. Functionally, overexpression and knockdown of HRF demonstrated the antiapoptotic effect of HRF in NHL cells, which may be associated with activation of the p-CREB/BCL-2 signaling pathway. Moreover, cell adhesion assay demonstrated that adhesion to fibronectin (FN) or HS-5 up-regulated HRF expression, while knockdown of HRF resulted in decreased cell adhesion, which led to reversed CAM-DR. Our finding supports the role of HRF in NHL cell apoptosis, adhesion and drug resistance, and may provide a clinical therapeutic target for CAM-DR in NHL.
authors.affiliation: Department of Oncology, Affiliated Cancer Hospital of Nantong University, Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target , Nantong, Jiangsu , China.
authors.forename: Song
authors.initials: S
authors.lastname: He
authors.forename: Yuejiao
authors.initials: Y
authors.lastname: Huang
authors.forename: Yuchan
authors.initials: Y
authors.lastname: Wang
authors.forename: Jie
authors.initials: J
authors.lastname: Tang
authors.forename: Yan
authors.initials: Y
authors.lastname: Song
authors.forename: Xiafei
authors.initials: X
authors.lastname: Yu
authors.forename: Jing
authors.initials: J
authors.lastname: Ma
authors.forename: Shitao
authors.initials: S
authors.lastname: Wang
authors.forename: Haibing
authors.initials: H
authors.lastname: Yin
authors.forename: Qiuyue
authors.initials: Q
authors.lastname: Li
authors.forename: Lili
authors.initials: L
authors.lastname: Ji
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Xu
mesh_terms: D017209:Apoptosis; D014408:Biomarkers, Tumor / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D015153:Blotting, Western; D002448:Cell Adhesion; D049109:Cell Proliferation; D019008:Drug Resistance, Neoplasm; D005434:Flow Cytometry; D006801:Humans; D007124:Immunoenzyme Techniques; D008228:Lymphoma, Non-Hodgkin / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology*; D034741:RNA, Small Interfering / Q000235:genetics; D015398:Signal Transduction; D014407:Tumor Cells, Cultured; D000090324:Tumor Protein, Translationally-Controlled 1
chemical_list: D014408:Biomarkers, Tumor; D034741:RNA, Small Interfering; D000090324:Tumor Protein, Translationally-Controlled 1
keywords: CAM-DR; HRF; Non-Hodgkin lymphoma (NHL); apoptosis; cell adhesion; immunohistochemistry