title: Effects of capsaicin on adipogenic differentiation in bovine bone marrow mesenchymal stem cell.
abstract: Capsaicin is a major constituent of hot chili peppers that influences lipid metabolism in animals. In this study, we explored the effects of capsaicin on adipogenic differentiation of bovine bone marrow mesenchymal stem cells (BMSCs) in a dose- and time-dependent manner. The BMSCs were treated with various concentrations of capsaicin (0, 0.1, 1, 5, and 10 μM) for 2, 4, and 6 days. Capsaicin suppressed fat deposition significantly during adipogenic differentiation. Peroxisome proliferator-activated receptor gamma, cytosine-cytosine-adenosine-adenosine-thymidine/enhancer binding protein alpha, fatty acid binding protein 4, and stearoyl-CoA desaturase expression decreased after capsaicin treatment. We showed that the number of apoptotic cells increased in dose- and time-dependent manners. Furthermore, we found that capsaicin increased the expression levels of apoptotic genes, such as B-cell lymphoma 2-associated X protein and caspase 3. Overall, capsaicin inhibits fat deposition by triggering apoptosis.
authors.affiliation: Animal Nutrition and Physiology Team, National Institute of Animal Science, Rural Development Administration, Suwon 441-706, Korea .
authors.forename: Jin Young
authors.initials: JY
authors.lastname: Jeong
authors.affiliation: Animal Nutrition and Physiology Team, National Institute of Animal Science, Rural Development Administration, Suwon 441-706, Korea .
authors.forename: Sekar
authors.initials: S
authors.lastname: Suresh
authors.affiliation: Animal Nutrition and Physiology Team, National Institute of Animal Science, Rural Development Administration, Suwon 441-706, Korea .
authors.forename: Mi Na
authors.initials: MN
authors.lastname: Park
authors.affiliation: Animal Nutrition and Physiology Team, National Institute of Animal Science, Rural Development Administration, Suwon 441-706, Korea .
authors.forename: Mi
authors.initials: M
authors.lastname: Jang
authors.affiliation: Animal Nutrition and Physiology Team, National Institute of Animal Science, Rural Development Administration, Suwon 441-706, Korea .
authors.forename: Sungkwon
authors.initials: S
authors.lastname: Park
authors.affiliation: Animal Nutrition and Physiology Team, National Institute of Animal Science, Rural Development Administration, Suwon 441-706, Korea .
authors.forename: Kuppannan
authors.initials: K
authors.lastname: Gobianand
authors.affiliation: The Laboratory of Cell Growth and Function Regulation, Division of Bioscience and Technology, College of Life and Environmental Sciences, Korea University, Seoul 136-701, Korea .
authors.forename: Seungkwon
authors.initials: S
authors.lastname: You
authors.affiliation: Animal Nutrition and Physiology Team, National Institute of Animal Science, Rural Development Administration, Suwon 441-706, Korea .
authors.forename: Sung-Heom
authors.initials: SH
authors.lastname: Yeon
authors.affiliation: Animal Nutrition and Physiology Team, National Institute of Animal Science, Rural Development Administration, Suwon 441-706, Korea .
authors.forename: Hyun-Jeong
authors.initials: HJ
authors.lastname: Lee
keywords: Adipogenesis; Apoptosis; Bovine Bone Marrow Mesenchymal Stem Cells [BMSCs]; Capsaicin; Differentiation