title: Investigation of functional activity human dental pulp stem cells at acute and chronic pulpitis.
abstract: It is already recognized that together with the other connective tissues organ-specific progenic stem cells are also found in postnatal dental pulp. This group of undifferentiated cells is only 1% of total cell population of the pulp. The aim of the study was the identification of stem cells in human dental pulp, detection of their localization and assessment of functional activity during inflammation process and/or at norm. The obtained results showed that at acute pulpitis the pulp stroma is hypocellular in comparison with the norm but cells proliferative activity is low. CD 133 and NCAM (CD 56) positive stem cells were found in perivascularl space of the pulp stroma and in Hohle layer. At process prolongation and transition to the chronic phase pulp stroma is hypercellular, the cells with large, rounded or oval-shaped nuclei with clear chromatin appear together with fibroblasts. They are distributed as about entire thickness of the stroma as especially Hohle layer. In such cells higher proliferative activity (Ki67 expression) was observed. The cells in the mentioned proliferation phase are intensively marked by CD133, the rate of which is high in Hohle layer and along it. A large number of NCAM (CD 56) positive cells appear in pulp stroma.
  
  
  CONCLUSIONS
  During pulpitis an involvement of stem cells into the process of reparative dentinogenesis should be conducted stepwise. In acute cases of the disease, stem cell perivascularl mobilization and proliferation and its migration to Hohle layer occur in response to irritation /stimulation. Chronification of the process leads not only to the migration of stem cells to the periphery of the pulp but also s their В«maturationВ» (increase of NCAM expression in the stem cells), which causes an increase the number of dentin producing active odontoblasts and initiation of reparative dentinogenesis.
authors.affiliation: Tbilisi State Medical University, Al. Natishvili Institute of Experimental Morphology; "UniDent" - Dental Clinic, Training and Research Center, Georgia.
authors.forename: M
authors.initials: M
authors.lastname: Ustiashvili
authors.affiliation: Tbilisi State Medical University, Al. Natishvili Institute of Experimental Morphology; "UniDent" - Dental Clinic, Training and Research Center, Georgia.
authors.forename: D
authors.initials: D
authors.lastname: Kordzaia
authors.affiliation: Tbilisi State Medical University, Al. Natishvili Institute of Experimental Morphology; "UniDent" - Dental Clinic, Training and Research Center, Georgia.
authors.forename: M
authors.initials: M
authors.lastname: Mamaladze
authors.affiliation: Tbilisi State Medical University, Al. Natishvili Institute of Experimental Morphology; "UniDent" - Dental Clinic, Training and Research Center, Georgia.
authors.forename: M
authors.initials: M
authors.lastname: Jangavadze
authors.affiliation: Tbilisi State Medical University, Al. Natishvili Institute of Experimental Morphology; "UniDent" - Dental Clinic, Training and Research Center, Georgia.
authors.forename: L
authors.initials: L
authors.lastname: Sanodze
mesh_terms: D000071916:AC133 Antigen; D015703:Antigens, CD / Q000096:biosynthesis; D002454:Cell Differentiation; D049109:Cell Proliferation; D003782:Dental Pulp / Q000378:metabolism / Q000473:pathology*; D003810:Dentinogenesis / Q000235:genetics*; D005260:Female; D006023:Glycoproteins / Q000096:biosynthesis; D006801:Humans; D019394:Ki-67 Antigen / Q000096:biosynthesis; D008297:Male; D019006:Neural Cell Adhesion Molecules / Q000096:biosynthesis; D009804:Odontoblasts / Q000378:metabolism / Q000473:pathology; D010455:Peptides; D011671:Pulpitis / Q000201:enzymology* / Q000235:genetics; D013234:Stem Cells / Q000378:metabolism*
chemical_list: D000071916:AC133 Antigen; D015703:Antigens, CD; D006023:Glycoproteins; D019394:Ki-67 Antigen; D019006:Neural Cell Adhesion Molecules; C000606023:PROM1 protein, human; D010455:Peptides