title: [TXNDC5 mediates serum starvation-induced proliferation inhibition of HeLa cell].
abstract: OBJECTIVE
  To investigate the role of TXNDC5 in serum starvation-induced proliferation inhibition of HeLa cell.
  
  
  METHODS
  TXNDC5 was either over-expressed or knocked down by small interfering RNA (siRNA) in HeLa cells which were then cultured in conventional medium or serum starvation medium. The protein level of TXNDC5 was evaluated by Western blot analysis. The mRNA level of TXNDC5 was measured by quantitative real-time PCR. Cell growth rate was determined by cell proliferation assay kit (MTS method). Cell cycle distribution and apoptosis were detected by flow cytometry.
  
  
  RESULTS
  Serum starvation mildly reduced the mRNA level of TXNDC5 (P<0.05), but dramatically increased the protein level of TXNDC5 in HeLa cells. The stability of TXNDC5 mRNA remained unchanged. Cycloheximide abolished the serum starvation-induced up-regulation of TXNDC5 protein. Over-expression of TXNDC5 had no effect on cell proliferation. However, suppression of TXNDC5 attenuated the proliferation inhibition of HeLa cell induced by serum starvation (P<0.05), increased the proportion of cells in S phase (P<0.05), but had no effect on cell apoptosis.
  
  
  CONCLUSIONS
  TXNDC5 mediates serum starvation-induced proliferation inhibition of HeLa cell.
authors.affiliation: State Key Laboratory of Medical Molecular Biology,Department of Biochemistry and Molecular Biology,Institute of Basic Medical Sciences,CAMS and PUMC,Beijing 100005,China.
authors.forename: Hong-fei
authors.initials: HF
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Medical Molecular Biology,Department of Biochemistry and Molecular Biology,Institute of Basic Medical Sciences,CAMS and PUMC,Beijing 100005,China.
authors.forename: Jie-wen
authors.initials: JW
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Medical Molecular Biology,Department of Biochemistry and Molecular Biology,Institute of Basic Medical Sciences,CAMS and PUMC,Beijing 100005,China.
authors.forename: Li-juan
authors.initials: LJ
authors.lastname: Kong
authors.affiliation: State Key Laboratory of Medical Molecular Biology,Department of Biochemistry and Molecular Biology,Institute of Basic Medical Sciences,CAMS and PUMC,Beijing 100005,China.
authors.forename: Le
authors.initials: L
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Medical Molecular Biology,Department of Biochemistry and Molecular Biology,Institute of Basic Medical Sciences,CAMS and PUMC,Beijing 100005,China.
authors.forename: Ning
authors.initials: N
authors.lastname: Zhu
authors.affiliation: State Key Laboratory of Medical Molecular Biology,Department of Biochemistry and Molecular Biology,Institute of Basic Medical Sciences,CAMS and PUMC,Beijing 100005,China.
authors.forename: Si-chao
authors.initials: SC
authors.lastname: Guo
authors.affiliation: State Key Laboratory of Medical Molecular Biology,Department of Biochemistry and Molecular Biology,Institute of Basic Medical Sciences,CAMS and PUMC,Beijing 100005,China.
authors.forename: Chuan
authors.initials: C
authors.lastname: Qiu
authors.affiliation: State Key Laboratory of Medical Molecular Biology,Department of Biochemistry and Molecular Biology,Institute of Basic Medical Sciences,CAMS and PUMC,Beijing 100005,China.
authors.forename: Xue-jing
authors.initials: XJ
authors.lastname: Yan
authors.affiliation: State Key Laboratory of Medical Molecular Biology,Department of Biochemistry and Molecular Biology,Institute of Basic Medical Sciences,CAMS and PUMC,Beijing 100005,China.
authors.forename: Mei-hong
authors.initials: MH
authors.lastname: Chen
mesh_terms: D017209:Apoptosis; D002453:Cell Cycle; D049109:Cell Proliferation; D003470:Culture Media / Q000737:chemistry; D055785:Gene Knockdown Techniques; D006367:HeLa Cells; D006801:Humans; D019704:Protein Disulfide-Isomerases / Q000235:genetics / Q000378:metabolism*; D044967:Serum / Q000737:chemistry
chemical_list: D003470:Culture Media; D019704:Protein Disulfide-Isomerases; C480743:TXNDC5 protein, human