title: Differences in the antibacterial activity of nisin and bovicin HC5 against Salmonella Typhimurium under different temperature and pH conditions.
abstract: OBJECTIVE
  To compare the action of nisin and bovicin HC5 in combination with EDTA on Salmonella Typhimurium under different environmental conditions.
  
  
  RESULTS
  Salmonella Typhimurium was treated in BHI broth containing EDTA (1·5 mmol l(-1)) and nisin or bovicin HC5 (200 AU ml(-1)) under different pH and temperature conditions, and according to a central composite design with two factors (temperature and pH). Cell viability was evaluated on plate count agar for 48 h. The combination of nisin or bovicin HC5 with EDTA was able to inhibit the growth of Salmonella, but the temperature and pH conditions promoting inhibition were distinct for each bacteriocin. Nisin was bactericidal over a broad range of temperature and pH, while bovicin HC5 was bacteriostatic in most conditions and bactericidal only in specific conditions (pH >6·0 and temperature >30°C). Salmonella Typhimurium did not show tolerance to bovicin HC5 or cross-tolerance between these lantibiotics.
  
  
  CONCLUSIONS
  Nisin and bovicin HC5 both inhibited the growth of Salmonella, but the activity of each bacteriocin was differently influenced by environmental conditions.
  
  
  CONCLUSIONS
  Nisin and bovicin HC5 have the potential to inhibit the growth of Salmonella, but environmental conditions should be considered to establish optimal conditions for its application.
authors.affiliation: Departamento de Microbiologia, Universidade Federal de Viçosa, Viçosa, Minas Gerais, Brazil.
authors.forename: C V
authors.initials: CV
authors.lastname: Prudêncio
authors.forename: H C
authors.initials: HC
authors.lastname: Mantovani
authors.forename: P R
authors.initials: PR
authors.lastname: Cecon
authors.forename: M C D
authors.initials: MC
authors.lastname: Vanetti
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001430:Bacteriocins / Q000494:pharmacology*; D004492:Edetic Acid / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D050296:Microbial Viability / Q000187:drug effects; D009561:Nisin / Q000494:pharmacology*; D012486:Salmonella typhimurium / Q000187:drug effects*; D013696:Temperature
chemical_list: D000900:Anti-Bacterial Agents; D001430:Bacteriocins; C514465:bovicin HC5, Streptococcus bovis HC5; D009561:Nisin; D004492:Edetic Acid
keywords: bacteriocins; biocontrol; environmental conditions; gram-negative bacteria; lantibiotics