title: Multilayered Inorganic Microparticles for Tunable Dual Growth Factor Delivery.
abstract: There is an increasing need to control the type, quantity, and timing of growth factors released during tissue healing. Sophisticated delivery systems offering the ability to deliver multiple growth factors with independently tunable kinetics are highly desirable. Here, a multilayered, mineral coated micro-particle (MCMs) platform that can serve as an adaptable dual growth factor delivery system is developed. Bone morphogenetic protein-2 (BMP-2) and vascular endothelial growth factor (VEGF) are bound to the mineral coatings with high binding efficiencies of up to 80%. BMP-2 is firstly bound onto a 1stmineral coating layer; then VEGF is bound onto a 2ndmineral coating layer. The release of BMP-2 is sustained over a period of 50 days while the release of VEGF is a typical two-phase release with rapid release in the first 14 days and more sustained release for the following 36 days. Notably, the release behaviors of both growth factors can be independently tailored by changing the intrinsic properties of the mineral coatings. Furthermore, the release of BMP-2 can be tuned by changing the thickness of the 2ndlayer. This injectable microparticle based delivery platform with tunable growth factor release has immense potential for applications in tissue engineering and regenerative medicine.
authors.affiliation: Department of Biomedical Engineering, University of Wisconsin, 1111 Highland Ave, Madison, WI, 53705, USA.
authors.forename: Xiaohua
authors.initials: X
authors.lastname: Yu
authors.affiliation: Department of Biomedical Engineering, University of Wisconsin, 1111 Highland Ave, Madison, WI, 53705, USA.
authors.forename: Andrew
authors.initials: A
authors.lastname: Khalil
authors.affiliation: Department of Biomedical Engineering and Orthopaedic Surgery, Case Western Reserve University, 10900 Euclid Avenue, Cleveland, OH, 44106, USA.
authors.forename: Phuong Ngoc
authors.initials: PN
authors.lastname: Dang
authors.affiliation: Department of Biomedical Engineering and Orthopaedic Surgery, Case Western Reserve University, 10900 Euclid Avenue, Cleveland, OH, 44106, USA.
authors.forename: Eben
authors.initials: E
authors.lastname: Alsberg
authors.affiliation: Department of Biomedical Engineering, University of Wisconsin, 1111 Highland Ave, Madison, WI, 53705, USA.
authors.forename: William L
authors.initials: WL
authors.lastname: Murphy