title: Characterization of an Anti-gout Xanthine Oxidase Inhibitor from Pleurotus ostreatus.
abstract: We selected Pleurotus ostreatus from among several edible mushrooms because it has high anti-gout xanthine oxidase (XOD) inhibitory activity. The maximal amount of XOD inhibitor was extracted when the Pleurotus ostreatus fruiting body was treated with distilled water at 40℃ for 48 hr. The XOD inhibitor thus obtained was purified by Sephadex G-50 gel permeation chromatography, ultrafiltration, C18 solid phase extraction chromatography and reverse-phase high-performance liquid chromatography with 3% of solid yield, and its XOD inhibitory activity was 0.9 mg/mL of IC50. The purified XOD inhibitor was a tripeptide with the amino acid sequence phenylalanine-cysteine-histidine and a molecular weight of 441.3 Da. The XOD inhibitor-containing ultrafiltrates from Pleurotus ostreatus demonstrated dose-dependent anti-gout effects in a Sprague-Dawley rat model of potassium oxonate-induced gout, as shown by decreased serum urated levels at doses of 500 and 1,000 mg/kg, although the effect was not as great as that achieved with the commercial anti-gout agent, allopurinol when administered at a dose of 50 mg/kg.
authors.affiliation: Department of Biomedicinal Science and Biotechnology, Paichai University, Daejeon 302-735, Korea.
authors.forename: In-Taek
authors.initials: IT
authors.lastname: Jang
authors.affiliation: Department of Biomedicinal Science and Biotechnology, Paichai University, Daejeon 302-735, Korea.
authors.forename: Se-Hee
authors.initials: SH
authors.lastname: Hyun
authors.affiliation: Department of Biomedicinal Science and Biotechnology, Paichai University, Daejeon 302-735, Korea.
authors.forename: Ja-Won
authors.initials: JW
authors.lastname: Shin
authors.affiliation: Mushroom Research Station, Gyeonggi-do Agricultural Research and Extension Services, Gwangju 464-873, Korea.
authors.forename: Yun-Hae
authors.initials: YH
authors.lastname: Lee
authors.affiliation: Mushroom Research Station, Gyeonggi-do Agricultural Research and Extension Services, Gwangju 464-873, Korea.
authors.forename: Jeong-Hyun
authors.initials: JH
authors.lastname: Ji
authors.affiliation: Department of Biomedicinal Science and Biotechnology, Paichai University, Daejeon 302-735, Korea.
authors.forename: Jong-Soo
authors.initials: JS
authors.lastname: Lee
keywords: Anti-gout xanthine oxidase inhibitor; Edible oyster mushroom; Pleurotus ostreatus