title: Simultaneous determination by UPLC-MS/MS of seven bioactive compounds in rat plasma after oral administration of Ginkgo biloba tablets: application to a pharmacokinetic study.
abstract: A rapid, reliable, and sensitive method was developed using ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) with an electrospray ionization (ESI) source for determination of seven bioactive compounds in rat plasma after oral administration of Ginkgo biloba tablets (GBTs). The method simultaneously detects bilobalide (BB), ginkgolide A (GA), ginkgolide B (GB), ginkgolide C (GC), quercetin (QCT), kaempferol (KMF), and isorhamnetin (ISR) for pharmacokinetic study. The analytes and internal standard (IS) were extracted from rat plasma by acetidin. An MS/MS detection was conducted using multiple reaction monitoring (MRM) and operating in the negative ionization mode. The calibration curve ranges were 5-500, 5-500, 2.5-250, 1-100, 1-100, 1-100, and 1-100 ng/ml for BB, GA, GB, GC, QCT, KMF, and ISR, respectively. The mean recovery of the analytes ranged from 68.11% to 84.42%. The intra- and inter-day precisions were in the range of 2.33%-9.86% and the accuracies were between 87.67% and 108.37%. The method was used successfully in a pharmacokinetic study of GBTs. The pharmacokinetic parameters of seven compounds were analyzed using a non-compartment model. Plasma concentrations of the seven compounds were determined up to 48 h after administration, and their pharmacokinetic parameters were in agreement with previous studies.
authors.affiliation: School of Chinese Material Medica, Beijing University of Chinese Medicine, Beijing 100102, China; Beijing Handian Pharmaceutical Co., Ltd., Beijing 100004, China.
authors.forename: Wen-ping
authors.initials: WP
authors.lastname: Wang
authors.forename: Na
authors.initials: N
authors.lastname: Liu
authors.forename: Qian
authors.initials: Q
authors.lastname: Kang
authors.forename: Pei-pei
authors.initials: PP
authors.lastname: Du
authors.forename: Yi
authors.initials: Y
authors.lastname: Lan
authors.forename: Bo-chen
authors.initials: BC
authors.lastname: Zhao
authors.forename: Yan-yan
authors.initials: YY
authors.lastname: Chen
authors.forename: Qing
authors.initials: Q
authors.lastname: Zhang
authors.forename: Hui
authors.initials: H
authors.lastname: Li
authors.forename: Ye-wen
authors.initials: YW
authors.lastname: Zhang
authors.forename: Qing
authors.initials: Q
authors.lastname: Wu
mesh_terms: D000284:Administration, Oral; D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods*; D004305:Dose-Response Relationship, Drug; D044948:Flavonols / Q000097:blood*; D020441:Ginkgo biloba / Q000737:chemistry*; D007783:Lactones / Q000097:blood*; D008297:Male; D010936:Plant Extracts / Q000008:administration & dosage / Q000493:pharmacokinetics*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D013607:Tablets; D013729:Terpenes / Q000097:blood
chemical_list: D044948:Flavonols; D007783:Lactones; D010936:Plant Extracts; D013607:Tablets; D013729:Terpenes
keywords: Ginkgo biloba tablet; Pharmacokinetics; Ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS)