title: Composition of diet modifies colonization dynamics of Campylobacter jejuni in broiler chickens.
abstract: OBJECTIVE
  To evaluate the impact of diet composition on colonization dynamics of Camp. jejuni and on related physiological parameters in the chicken intestine.
  
  
  RESULTS
  A total of 54 1-day-old Ross 308 broiler chicks were randomly divided into three isocaloric and isonitrogenous dietary groups: maize-based (MB), wheat-based (WB) diet and wheat-based diet with NSP-degrading enzyme supplementation (WBES). Chickens were orally infected with 10(8)  CFU Camp. jejuni on day 14, and samples (n = 6) were collected on 7, 14 and 21 days postinfection (DPI), respectively. Colony forming units of Camp. jejuni of caecum and jejunum, short-chain fatty acid (SCFA) concentrations, pH values of the caecum, jejunal histomorphology and viscosity of jejunal chymus were measured. In case of WBES diet, lower Camp. jejuni colonization 14 DPI, higher jejunal viscosity, higher total SCFA concentrations in the caecum and enhanced jejunal histomorphology were observed compared to those measured in chickens fed MB diet.
  
  
  CONCLUSIONS
  The WBES diet altered Camp. jejuni colonization dynamics in the chicken intestine which resulted by higher SCFA concentrations in the caecum and by the change of gut morphology.
  
  
  CONCLUSIONS
  Our study proves that diet composition can modify Camp. jejuni colonization depending on sampling time point postinfection.
authors.affiliation: Department of Animal Science and Animal Husbandry, Georgikon Faculty, University of Pannonia, Keszthely, Hungary.
authors.forename: A
authors.initials: A
authors.lastname: Molnár
authors.forename: C
authors.initials: C
authors.lastname: Hess
authors.forename: L
authors.initials: L
authors.lastname: Pál
authors.forename: L
authors.initials: L
authors.lastname: Wágner
authors.forename: W A
authors.initials: WA
authors.lastname: Awad
authors.forename: F
authors.initials: F
authors.lastname: Husvéth
authors.forename: M
authors.initials: M
authors.lastname: Hess
authors.forename: K
authors.initials: K
authors.lastname: Dublecz
mesh_terms: D000818:Animals; D016123:Campylobacter jejuni / Q000254:growth & development*; D002432:Cecum / Q000737:chemistry / Q000382:microbiology; D002645:Chickens / Q000382:microbiology*; D004032:Diet; D005232:Fatty Acids, Volatile / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D007583:Jejunum / Q000033:anatomy & histology / Q000382:microbiology / Q000502:physiology; D014908:Triticum; D014783:Viscosity; D003313:Zea mays
chemical_list: D005232:Fatty Acids, Volatile
keywords: Campylobacter jejuni; broiler chicken; enzyme; histomorphology; poultry; short-chain fatty acids; viscosity; wheat