title: Comparative Analysis of Sorghum bicolor Proteome in Response to Drought Stress and following Recovery.
abstract: The adaptive response of Sorghum bicolor landraces from Egypt to drought stress and following recovery was analyzed using two-dimensional difference gel electrophoresis, 2D-DIGE. Physiological measurements and proteome alterations of accession number 11434, drought tolerant, and accession number 11431, drought sensitive, were compared to their relative control values after drought stress and following recovery. Differentially expressed proteins were analysed by Matrix assisted laser desorption ionisation time-of-flight mass spectrometry, MALDI-TOF-MS. Alterations in protein contents related to the energy balance, metabolism (sensu Mewes et al. 1997), and chaperons were the most apparent features to elucidate the differences between the drought tolerant and sensitive accessions. Further alterations in the levels of proteins related to transcription and protein synthesis are discussed.
authors.affiliation: Institute of Ecology, Evolution, and Diversity, Johann Wolfgang Goethe-University Frankfurt, Max-Von-Laue Straße 13, 60438 Frankfurt, Germany.
authors.forename: Christoph
authors.identifier: 0000-0003-4647-4896
authors.initials: C
authors.lastname: Jedmowski
authors.affiliation: Institute of Ecology, Evolution, and Diversity, Johann Wolfgang Goethe-University Frankfurt, Max-Von-Laue Straße 13, 60438 Frankfurt, Germany ; Biodiversity and Climate Research Centre (BiK-F), 60325, Frankfurt am Main, Germany ; Agricultural Genetic Engineering Research Institute (AGERI), ARC, Giza 12619, Egypt.
authors.forename: Ahmed
authors.initials: A
authors.lastname: Ashoub
authors.affiliation: Institute of Pharmaceutical Chemistry, Johann Wolfgang Goethe-University Frankfurt, 60438 Frankfurt, Germany.
authors.forename: Tobias
authors.initials: T
authors.lastname: Beckhaus
authors.affiliation: Biodiversity and Climate Research Centre (BiK-F), 60325, Frankfurt am Main, Germany.
authors.forename: Thomas
authors.identifier: 0000-0001-9442-4450
authors.initials: T
authors.lastname: Berberich
authors.affiliation: Institute of Pharmaceutical Chemistry, Johann Wolfgang Goethe-University Frankfurt, 60438 Frankfurt, Germany.
authors.forename: Michael
authors.initials: M
authors.lastname: Karas
authors.affiliation: Institute of Ecology, Evolution, and Diversity, Johann Wolfgang Goethe-University Frankfurt, Max-Von-Laue Straße 13, 60438 Frankfurt, Germany ; Biodiversity and Climate Research Centre (BiK-F), 60325, Frankfurt am Main, Germany.
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Brüggemann