title: [Establishment of an induced pluripotent stem cell line from a patient with acute lung injury].
abstract: OBJECTIVE
  To establish an induced pluripotent stem (iPS) cell line from the skin fibroblasts of a patient with acute lung injury (ALI-iPS).
  
  
  METHODS
  We obtained iPS cells from a female ALI patient by forced expression of a cocktail of transcription factors (Sox2, Klf4, Oct4 and Nanog) into the dermal fibroblasts to result in reprogramming to a pluripotent state. The ALI-iPS cells generated were analyzed for colony morphology, alkaline phosphatase (AP) activity, surface antigens, and differentiation ability.
  
  
  RESULTS
  The ALI-iPS cells exhibited morphological and growth characteristics of ES cells, showed positivity for alkaline phosphatase by histochemical staining, and expressed ES cell marker genes. Subcutaneous injection of iPS cells into immunodeficient (SCID) mice resulted in tumors containing a variety of tissues from all the 3 germ layers.
  
  
  CONCLUSIONS
  The ALI patient-specific iPS cell line was successfully established to serve as a valuable model to study the cellular pathology of acute lung injury and develop high-throughput drug screening assays.
authors.affiliation: Pharmaceutical College of Kunming Medical University, Kunming 650031, China.E-mail: dt101180@sina.com.
authors.forename: Tao
authors.initials: T
authors.lastname: Ding
authors.forename: Yang
authors.initials: Y
authors.lastname: Li
authors.forename: Ruizhu
authors.initials: R
authors.lastname: Tang
authors.forename: Xuan
authors.initials: X
authors.lastname: Zhang
authors.forename: Yu
authors.initials: Y
authors.lastname: Yun
authors.forename: Jian
authors.initials: J
authors.lastname: Li
authors.forename: Dianhu
authors.initials: D
authors.lastname: Twang
mesh_terms: D055371:Acute Lung Injury; D000818:Animals; D002460:Cell Line; D065150:Cellular Reprogramming; D003470:Culture Media / Q000737:chemistry; D005260:Female; D005347:Fibroblasts / Q000166:cytology; D015870:Gene Expression; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology*; D000090062:Kruppel-Like Factor 4; D051379:Mice; D016513:Mice, SCID; D012867:Skin / Q000166:cytology; D014157:Transcription Factors / Q000737:chemistry
chemical_list: D003470:Culture Media; C000715467:KLF4 protein, human; C000715468:Klf4 protein, mouse; D000090062:Kruppel-Like Factor 4; D014157:Transcription Factors