title: Human bone marrow mesenchymal progenitors: perspectives on an optimized in vitro manipulation.
abstract: When it comes to regenerative medicine, mesenchymal stem cells (MSCs) are considered one of the most promising cell types for use in many cell therapies and bioengineering protocols. The International Society of Cellular Therapy recommended minimal criteria for defining multipotential MSC is based on adhesion and multipotency in vitro, and the presence or absence of select surface markers. Though these criteria help minimize discrepancies and allow some comparisons of data generated in different laboratories, the conditions in which cells are isolated and expanded are often not considered. Herein, we propose and recommend a few procedures to be followed to facilitate the establishment of quality control standards when working with mesenchymal progenitors isolation and expansion. Following these procedures, the classic Colony-Forming Unit-Fibroblast (CFU-f) assay is revisited and three major topics are considered to define conditions and to assist on protocol optimization and data interpretation. We envision that the creation of a guideline will help in the identification and isolation of long-term stem cells and short-term progenitors to better explore their regenerative potential for multiple therapeutic purposes.
authors.affiliation: LaBioTeC, Universidade Veiga de Almeida Rio de Janeiro, Brazil.
authors.forename: Eric
authors.initials: E
authors.lastname: Cordeiro-Spinetti
authors.affiliation: LaBioTeC, Universidade Veiga de Almeida Rio de Janeiro, Brazil ; Laboratório de Pesquisas sobre o Timo, Instituto Oswaldo Cruz Fiocruz, Rio de Janeiro, Brazil.
authors.forename: Wallace
authors.initials: W
authors.lastname: de Mello
authors.affiliation: Department of Biological Sciences, Tokyo Metropolitan University Hachioji, Tokyo, Japan.
authors.forename: Lucas Siqueira
authors.initials: LS
authors.lastname: Trindade
authors.affiliation: Department of Vetarans Affairs, Hematology and Immunology Research, Washington DC Veterans Affairs Medical Center Washington, DC, USA.
authors.forename: Dennis D
authors.initials: DD
authors.lastname: Taub
authors.affiliation: School of Dentistry, Department of Periodontics and Oral Medicine, University of Michigan Ann Arbor, MI, USA.
authors.forename: Russell S
authors.initials: RS
authors.lastname: Taichman
authors.affiliation: LaBioTeC, Universidade Veiga de Almeida Rio de Janeiro, Brazil ; Excellion Serviços Biomédicos Petrópolis, Rio de Janeiro, Brazil.
authors.forename: Alex
authors.initials: A
authors.lastname: Balduino
keywords: bone marrow; colony; in vitro expansion; mesenchymal stem cell; mutipotent progenitor