title: [Applicability of laser-based geological techniques in bone research: analysis of calcium oxide distribution in thin-cut animal bones].
abstract: The structural similarities between the inorganic component of bone tissue and geological formations make it possible that mathematic models may be used to determine weight percentage composition of different mineral element oxides constituting the inorganic component of bone tissue. The determined weight percentage composition can be verified with the determination of element oxide concentration values by laser induced plasma spectroscopy and inductively coupled plasma optical emission spectrometry. It can be concluded from calculated weight percentage composition of the inorganic component of bone tissue and laboratory analyses that the properties of bone tissue are determined primarily by hydroxylapatite. The inorganic bone structure can be studied well by determining the calcium oxide concentration distribution using the laser induced plasma spectroscopy technique. In the present study, thin polished bone slides prepared from male bovine tibia were examined with laser induced plasma spectroscopy in a regular network and combined sampling system to derive the calculated calcium oxide concentration distribution. The superficial calcium oxide concentration distribution, as supported by "frequency distribution" curves, can be categorized into a number of groups. This, as such, helps in clearly demarcating the cortical and trabecular bone structures. Following analyses of bovine tibial bone, the authors found a positive association between the attenuation value, as determined by quantitative computer tomography and the "ρ" density, as used in geology. Furthermore, the calculated "ρ" density and the measured average calcium oxide concentration values showed inverse correlation.
authors.affiliation: Magyar Földtani és Geofizikai Intézet Budapest.
authors.forename: László
authors.initials: L
authors.lastname: Andrássy
authors.affiliation: Magyar Földtani és Geofizikai Intézet Budapest.
authors.forename: Gyula
authors.initials: G
authors.lastname: Maros
authors.affiliation: Magyar Földtani és Geofizikai Intézet Budapest.
authors.forename: István János
authors.initials: IJ
authors.lastname: Kovács
authors.affiliation: Debreceni Egyetem, Általános Orvostudományi Kar Reumatológiai Tanszék Debrecen Nagyerdei krt. 98. 4032.
authors.forename: Ágnes
authors.initials: Á
authors.lastname: Horváth
authors.affiliation: Debreceni Egyetem, Általános Orvostudományi Kar Reumatológiai Tanszék Debrecen Nagyerdei krt. 98. 4032.
authors.forename: Katalin
authors.initials: K
authors.lastname: Gulyás
authors.affiliation: Magyar Földtani és Geofizikai Intézet Budapest.
authors.forename: Éva
authors.initials: É
authors.lastname: Bertalan
authors.affiliation: Magyar Földtani és Geofizikai Intézet Budapest.
authors.forename: Anikó
authors.initials: A
authors.lastname: Besnyi
authors.affiliation: Magyar Földtani és Geofizikai Intézet Budapest.
authors.forename: Judit
authors.initials: J
authors.lastname: Füri
authors.affiliation: Magyar Földtani és Geofizikai Intézet Budapest.
authors.forename: Tamás
authors.initials: T
authors.lastname: Fancsik
authors.affiliation: Debreceni Egyetem, Általános Orvostudományi Kar Reumatológiai Tanszék Debrecen Nagyerdei krt. 98. 4032.
authors.forename: Zoltán
authors.initials: Z
authors.lastname: Szekanecz
authors.affiliation: Debreceni Egyetem, Általános Orvostudományi Kar Laboratóriumi Medicina Intézet Debrecen.
authors.forename: Harjit Pal
authors.initials: HP
authors.lastname: Bhattoa
mesh_terms: D000818:Animals; D001857:Bone Matrix / Q000737:chemistry; D001842:Bone and Bones / Q000737:chemistry*; D017610:Calcium Compounds / Q000032:analysis*; D002417:Cattle; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D005844:Geology; D007834:Lasers; D008297:Male; D008962:Models, Theoretical; D010087:Oxides / Q000032:analysis*; D014018:Tissue Distribution; D014131:Trace Elements / Q000032:analysis*
chemical_list: D017610:Calcium Compounds; D010087:Oxides; D014131:Trace Elements; C016538:lime
keywords: bone tissue; calcium oxide; csontszövet; kalcium-oxid; laser induced plasma spectroscopy; lézerindukált plazmaspektroszkópia; mineral composition; ásványok összetétele