title: Biomarkers for monitoring pre-analytical quality variation of mRNA in blood samples.
abstract: There is an increasing need for proper quality control tools in the pre-analytical phase of the molecular diagnostic workflow. The aim of the present study was to identify biomarkers for monitoring pre-analytical mRNA quality variations in two different types of blood collection tubes, K2EDTA (EDTA) tubes and PAXgene Blood RNA Tubes (PAXgene tubes). These tubes are extensively used both in the diagnostic setting as well as for research biobank samples. Blood specimens collected in the two different blood collection tubes were stored for varying times at different temperatures, and microarray analysis was performed on resultant extracted RNA. A large set of potential mRNA quality biomarkers for monitoring post-phlebotomy gene expression changes and mRNA degradation in blood was identified. qPCR assays for the potential biomarkers and a set of relevant reference genes were generated and used to pre-validate a sub-set of the selected biomarkers. The assay precision of the potential qPCR based biomarkers was determined, and a final validation of the selected quality biomarkers using the developed qPCR assays and blood samples from 60 healthy additional subjects was performed. In total, four mRNA quality biomarkers (USP32, LMNA, FOSB, TNRFSF10C) were successfully validated. We suggest here the use of these blood mRNA quality biomarkers for validating an experimental pre-analytical workflow. These biomarkers were further evaluated in the 2nd ring trial of the SPIDIA-RNA Program which demonstrated that these biomarkers can be used as quality control tools for mRNA analyses from blood samples.
authors.affiliation: DiaGenic ASA, Oslo, Norway.
authors.forename: Hui
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Laboratory of Gene Expression, Institute of Biotechnology, Academy of Sciences of the Czech Republic, Prague, Czech Republic.
authors.forename: Vlasta
authors.initials: V
authors.lastname: Korenková
authors.affiliation: TATAA Biocenter, Gothenburg, Sweden.
authors.forename: Robert
authors.initials: R
authors.lastname: Sjöback
authors.affiliation: Laboratory of Gene Expression, Institute of Biotechnology, Academy of Sciences of the Czech Republic, Prague, Czech Republic; TATAA Biocenter, Gothenburg, Sweden.
authors.forename: David
authors.initials: D
authors.lastname: Švec
authors.affiliation: TATAA Biocenter, Gothenburg, Sweden.
authors.forename: Jens
authors.initials: J
authors.lastname: Björkman
authors.affiliation: AROS Applied Biotechnology AS, Aarhus, Denmark.
authors.forename: Mogens
authors.initials: M
authors.lastname: Kruhøffer
authors.affiliation: Fondazione IRCCS Istituto Nazionale dei Tumori di Milano, Milano, Italy.
authors.forename: Paolo
authors.initials: P
authors.lastname: Verderio
authors.affiliation: Fondazione IRCCS Istituto Nazionale dei Tumori di Milano, Milano, Italy.
authors.forename: Sara
authors.initials: S
authors.lastname: Pizzamiglio
authors.affiliation: Fondazione IRCCS Istituto Nazionale dei Tumori di Milano, Milano, Italy.
authors.forename: Chiara Maura
authors.initials: CM
authors.lastname: Ciniselli
authors.affiliation: Qiagen GmbH, Hilden, Germany.
authors.forename: Ralf
authors.initials: R
authors.lastname: Wyrich
authors.affiliation: Qiagen GmbH, Hilden, Germany.
authors.forename: Uwe
authors.initials: U
authors.lastname: Oelmueller
authors.affiliation: Laboratory of Gene Expression, Institute of Biotechnology, Academy of Sciences of the Czech Republic, Prague, Czech Republic; TATAA Biocenter, Gothenburg, Sweden.
authors.forename: Mikael
authors.initials: M
authors.lastname: Kubista
authors.affiliation: DiaGenic ASA, Oslo, Norway.
authors.forename: Torbjørn
authors.initials: T
authors.lastname: Lindahl
authors.affiliation: DiaGenic ASA, Oslo, Norway.
authors.forename: Anders
authors.initials: A
authors.lastname: Lönneborg
authors.affiliation: DiaGenic ASA, Oslo, Norway.
authors.forename: Edith
authors.initials: E
authors.lastname: Rian
mesh_terms: D001800:Blood Specimen Collection; D020869:Gene Expression Profiling; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis; D016133:Polymerase Chain Reaction; D020871:RNA Stability; D012333:RNA, Messenger / Q000097:blood* / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification
chemical_list: D012333:RNA, Messenger