title: Increased TRPP2 expression in vascular smooth muscle cells from high-salt intake hypertensive rats: The crucial role in vascular dysfunction.
abstract: METHODS
  High-salt intake is a major risk factor in the development of hypertension. The underlying mechanism of high sodium on the cardiovascular system has received extensive attention. TRPP2 (Polycystin-2) is a Ca(2+) permeable nonselective cation channel that mediates Ca(2+) mobilization to control vascular smooth muscle cells (VSMCs) contraction. Here, we investigated TRPP2 expression change in VSMCs from high-salt intake hypertensive rats and role of TRPP2 in the development of high-salt diet-induced hypertension.
  
  
  RESULTS
  After 4 ws of dietary treatment, systolic blood pressure was significantly elevated in high-salt intake rats (132 ± 3 mmHg) compared with regular diet control rats (104 ± 2 mmHg). Results from vessel tension and diameter measurements show that high-salt intake potentiated phenylephrine-induced contraction in denuded mesenteric artery and thoracic aorta. Immunoblot and immunofluorescence data indicate that TRPP2 expression in VSMCs from mesenteric artery and thoracic aorta was significantly increased in high-salt intake-induced hypertensive rats. However, agonist-induced contractions in denuded mesenteric artery and thoracic aorta were markedly decreased if TRPP2 was knocked down by specific shRNA.
  
  
  CONCLUSIONS
  Our data demonstrate that high-salt intake increased TRPP2 expression in VSMCs, which in turn potentiated blood vessel response to contractors; this may participate in the development of hypertension.
authors.affiliation: Department of Physiology, Anhui Medical University, Hefei, Anhui, P. R. China; Department of Pharmacology, Anhui Medical University, Hefei, Anhui, P. R. China; Department of Cardiology, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui, P. R. China.
authors.forename: Ren
authors.initials: R
authors.lastname: Zhao
authors.forename: Muyao
authors.initials: M
authors.lastname: Zhou
authors.forename: Jie
authors.initials: J
authors.lastname: Li
authors.forename: Xia
authors.initials: X
authors.lastname: Wang
authors.forename: Kunli
authors.initials: K
authors.lastname: Su
authors.forename: Juncheng
authors.initials: J
authors.lastname: Hu
authors.forename: Yong
authors.initials: Y
authors.lastname: Ye
authors.forename: Jinhang
authors.initials: J
authors.lastname: Zhu
authors.forename: Gongliang
authors.initials: G
authors.lastname: Zhang
authors.forename: Kai
authors.initials: K
authors.lastname: Wang
authors.forename: Juan
authors.initials: J
authors.lastname: Du
authors.forename: Liecheng
authors.initials: L
authors.lastname: Wang
authors.forename: Bing
authors.initials: B
authors.lastname: Shen
mesh_terms: D000818:Animals; D001794:Blood Pressure / Q000187:drug effects; D006973:Hypertension / Q000235:genetics*; D008638:Mesenteric Arteries / Q000187:drug effects; D009119:Muscle Contraction / Q000187:drug effects; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000187:drug effects; D032389:Myocytes, Smooth Muscle / Q000187:drug effects* / Q000378:metabolism; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism; D051381:Rats; D012307:Risk Factors; D012982:Sodium, Dietary / Q000009:adverse effects*; D050396:TRPP Cation Channels / Q000235:genetics / Q000378:metabolism*
chemical_list: D034741:RNA, Small Interfering; D012982:Sodium, Dietary; D050396:TRPP Cation Channels
keywords: High-salt intake; Hypertension; TRPP2; Vascular smooth muscle cell; Vessel tension