title: Optimization of process parameters of extraction of amentoflavone, quercetin and ginkgetin from Taxus chinensis using supercritical CO2 plus co-solvent.
abstract: The effects of extraction time, temperature, pressure and different concentration of ethanol and their interactions on the yields of amentoflavone, quercetin and ginkgetin extracted from Taxus chinensis by supercritical CO2 were investigated by using a central composite design (CCD). An CCD experimental design with four factors and five levels was used to optimize the extraction parameters. Ultra performance liquid chromatography (UPLC) was used to analyze the content of the tree components in the extracts. Experimental results show that the main effects of factors and their interactions are significant on the yields (p < 0.05). The optimal extraction conditions were established for the three compounds: yield of 4.47 mg/g for amentoflavone at 48 °C, 25 MPa, 2.02 h and 78.5% ethanol, 3.73 mg/g for quercetin at 46 °C, 24 MPa, 2.3 h, 82% ethanol and 3.47 mg/g for ginkgetin at 48 °C, 20 MPa, 2.38 h, 82% ethanol, respectively.
authors.affiliation: Ningbo Institute of Technology, Zhejiang University, Ningbo, 315100, China. ruanxiao@nit.zju.edu.cn.
authors.forename: Xiao
authors.initials: X
authors.lastname: Ruan
authors.affiliation: Ningbo Institute of Technology, Zhejiang University, Ningbo, 315100, China. yanliuye@163.com.
authors.forename: Liu-Ye
authors.initials: LY
authors.lastname: Yan
authors.affiliation: Ningbo Institute of Technology, Zhejiang University, Ningbo, 315100, China. lixianxian@163.com.
authors.forename: Xian-Xian
authors.initials: XX
authors.lastname: Li
authors.affiliation: Ningbo Institute of Technology, Zhejiang University, Ningbo, 315100, China. liuben@nit.zju.edu.cn.
authors.forename: Ben
authors.initials: B
authors.lastname: Liu
authors.affiliation: Ningbo Institute of Technology, Zhejiang University, Ningbo, 315100, China. zhhuan@nit.zju.edu.cn.
authors.forename: Huan
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Ningbo Institute of Technology, Zhejiang University, Ningbo, 315100, China. wangqiangsky@263.net.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Wang
mesh_terms: D044946:Biflavonoids / Q000737:chemistry*; D002245:Carbon Dioxide / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D000431:Ethanol / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry*; D011312:Pressure; D011794:Quercetin / Q000737:chemistry*; D012997:Solvents / Q000737:chemistry*; D020946:Taxus / Q000737:chemistry*; D013696:Temperature
chemical_list: D044946:Biflavonoids; D010936:Plant Extracts; D012997:Solvents; D002245:Carbon Dioxide; D000431:Ethanol; C077458:ginkgetin; C011164:amentoflavone; D011794:Quercetin