title: Fluorescent correlation spectroscopy measurements with adaptive optics in the intercellular space of spheroids.
abstract: In this study we demonstrate the use of adaptive optics to correct the biasing effects of optical aberrations when measuring the dynamics of molecules diffusing between cells in multicellular spheroids. Our results indicate that, on average, adaptive optics leads to a reduction of the 3D size of the point spread function that is statistically significant in terms of measured number of molecules and diffusion time. The sensorless approach, which uses the molecular brightness as optimization metric, is validated in a complex, highly heterogeneous, biological environment. This work paves the way towards the design of accurate diffusion measurements of molecules in thick biological specimens.
authors.affiliation: Univ. Grenoble Alpes, LIPHY, F-38000 Grenoble, France ; CNRS, LIPHY, F-38000 Grenoble, France ; Institut Langevin, ESPCI ParisTech, CNRS, 1 rue Jussieu, 75238 Paris Cedex 05, France.
authors.forename: Charles-Edouard
authors.initials: CE
authors.lastname: Leroux
authors.affiliation: UMR 168, Institut Curie, Centre de Recherche, 26 rue d'Ulm 75005 Paris, France.
authors.forename: Sylvain
authors.initials: S
authors.lastname: Monnier
authors.affiliation: Univ. Grenoble Alpes, LIPHY, F-38000 Grenoble, France ; CNRS, LIPHY, F-38000 Grenoble, France.
authors.forename: Irène
authors.initials: I
authors.lastname: Wang
authors.affiliation: Univ. Grenoble Alpes, LIPHY, F-38000 Grenoble, France ; CNRS, LIPHY, F-38000 Grenoble, France.
authors.forename: Giovanni
authors.initials: G
authors.lastname: Cappello
authors.affiliation: Univ. Grenoble Alpes, LIPHY, F-38000 Grenoble, France ; CNRS, LIPHY, F-38000 Grenoble, France.
authors.forename: Antoine
authors.initials: A
authors.lastname: Delon
keywords: (010.1080) Active or adaptive optics; (170.6280) Spectroscopy, fluorescence and luminescence; (180.1790) Confocal microscopy; (180.2520) Fluorescence microscopy