title: Factors affecting the development of somatic cell nuclear transfer embryos in Cattle.
abstract: Nuclear transfer is a complex multistep procedure that includes oocyte maturation, cell cycle synchronization of donor cells, enucleation, cell fusion, oocyte activation and embryo culture. Therefore, many factors are believed to contribute to the success of embryo development following nuclear transfer. Numerous attempts to improve cloning efficiency have been conducted since the birth of the first sheep by somatic cell nuclear transfer. However, the efficiency of somatic cell cloning has remained low, and applications have been limited. In this review, we discuss some of the factors that affect the developmental ability of somatic cell nuclear transfer embryos in cattle.
authors.affiliation: Animal Breeding and Reproduction Research Division, NARO Institute of Livestock and Grassland Science, Ibaraki 305-0901, Japan.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Akagi
authors.forename: Kazutsugu
authors.initials: K
authors.lastname: Matsukawa
authors.forename: Seiya
authors.initials: S
authors.lastname: Takahashi
mesh_terms: D000818:Animals; D001363:Awards and Prizes; D001755:Blastocyst / Q000166:cytology / Q000502:physiology; D002417:Cattle / Q000196:embryology*; D002452:Cell Count; D002453:Cell Cycle; D002459:Cell Fusion / Q000662:veterinary; D002478:Cells, Cultured; D019976:Cloning, Organism / Q000379:methods / Q000662:veterinary; D047108:Embryonic Development; D056572:Histone Deacetylase Inhibitors / Q000494:pharmacology; D059471:In Vitro Oocyte Maturation Techniques; D053652:Nuclear Transfer Techniques / Q000662:veterinary*; D009865:Oocytes / Q000502:physiology; D012098:Reproduction
chemical_list: D056572:Histone Deacetylase Inhibitors