title: Prognostic significance of SERPINE2 in gastric cancer and its biological function in SGC7901 cells.
abstract: OBJECTIVE
  Altered expression of serine protease inhibitor peptidase inhibitor clade E member 2 (SERPINE2) associates with human cancer development and progression; thus, this study investigated SERPINE2 expression in gastric cancer tissues for association with clinicopathological and survival data from the patients and then investigated the role of SERPINE2 in gastric cancer cells in vitro.
  
  
  METHODS
  The levels of SERPINE2 mRNA in 243 gastric cancer tissues and paired non-cancerous mucosa were determined using quantitative PCR. Inhibition of SERPINE2 expression by small interfering RNA (siRNA) was detected by Western blotting. tetrazolium, soft agar, and transwell assays were performed to evaluate the proliferation, anchorage-independent growth, and motility of gastric cancer SGC7901 cells transfected with SERPINE2 siRNA.
  
  
  RESULTS
  Compared with the normal mucosa, SERPINE2 mRNA was increased in gastric cancer tissues and cells. Analysis of the 243 matched specimens showed that high SERPINE2 levels were associated with lymph node metastasis, distant metastasis, and clinical stage. Patients with high SERPINE2 mRNA levels had poorer survival compared with patients with low SERPINE2 mRNA levels. In vitro, SERPINE2 inhibited anchorage-independent growth, migration, and invasion of SGC7901 cells, but not proliferation.
  
  
  CONCLUSIONS
  Our findings indicate that upregulated SERPINE2 may contribute to the aggressive phenotype of gastric cancer and suggest that SERPINE2 can be used as a novel prognostic factor and anticancer target in patients with gastric cancer.
authors.affiliation: Department of General Surgery, Qianfoshan Hospital, Shandong University, 16766 Jing Shi Road, Jinan, 250014, China.
authors.forename: Kun
authors.initials: K
authors.lastname: Wang
authors.forename: Bin
authors.initials: B
authors.lastname: Wang
authors.forename: Ai Yan
authors.initials: AY
authors.lastname: Xing
authors.forename: Ke Sen
authors.initials: KS
authors.lastname: Xu
authors.forename: Guang Xin
authors.initials: GX
authors.lastname: Li
authors.forename: Zhen Hai
authors.initials: ZH
authors.lastname: Yu
mesh_terms: D000328:Adult; D000368:Aged; D015153:Blotting, Western; D045744:Cell Line, Tumor; D002465:Cell Movement; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D008875:Middle Aged; D010641:Phenotype; D016133:Polymerase Chain Reaction; D011237:Predictive Value of Tests; D011379:Prognosis; D034741:RNA, Small Interfering / Q000378:metabolism; D058258:Serpin E2 / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D013274:Stomach Neoplasms / Q000378:metabolism*; D015854:Up-Regulation
chemical_list: D034741:RNA, Small Interfering; C545486:SERPINE2 protein, human; D058258:Serpin E2