title: The dual role of scavenger receptor class A in development of diabetes in autoimmune NOD mice.
abstract: Human type 1 diabetes is an autoimmune disease that results from the autoreactive destruction of pancreatic β cells by T cells. Antigen presenting cells including dendritic cells and macrophages are required to activate and suppress antigen-specific T cells. It has been suggested that antigen uptake from live cells by dendritic cells via scavenger receptor class A (SR-A) may be important. However, the role of SR-A in autoimmune disease is unknown. In this study, SR-A-/- nonobese diabetic (NOD) mice showed significant attenuation of insulitis, lower levels of insulin autoantibodies, and suppression of diabetes development compared with NOD mice. We also found that diabetes progression in SR-A-/- NOD mice treated with low-dose polyinosinic-polycytidylic acid (poly(I:C)) was significantly accelerated compared with that in disease-resistant NOD mice treated with low-dose poly(I:C). In addition, injection of high-dose poly(I: C) to mimic an acute RNA virus infection significantly accelerated diabetes development in young SR-A-/- NOD mice compared with untreated SR-A-/- NOD mice. Pathogenic cells including CD4+CD25+ activated T cells were increased more in SR-A-/- NOD mice treated with poly(I:C) than in untreated SR-A-/- NOD mice. These results suggested that viral infection might accelerate diabetes development even in diabetes-resistant subjects. In conclusion, our studies demonstrated that diabetes progression was suppressed in SR-A-/- NOD mice and that acceleration of diabetes development could be induced in young mice by poly(I:C) treatment even in SR-A-/- NOD mice. These results suggest that SR-A on antigen presenting cells such as dendritic cells may play an unfavorable role in the steady state and a protective role in a mild infection. Our findings imply that SR-A may be an important target for improving therapeutic strategies for type 1 diabetes.
authors.affiliation: Department of General Internal Medicine, Kobe University Graduate School of Medicine, Chuo-ku, Kobe, Japan.
authors.forename: Mami
authors.initials: M
authors.lastname: Shimizu
authors.affiliation: Department of General Internal Medicine, Kobe University Graduate School of Medicine, Chuo-ku, Kobe, Japan; Division of Health Sciences, Department of Community Health Sciences, Kobe University Graduate School of Health Sciences, Suma-ku, Kobe, Japan.
authors.forename: Hisafumi
authors.initials: H
authors.lastname: Yasuda
authors.affiliation: Department of General Internal Medicine, Kobe University Graduate School of Medicine, Chuo-ku, Kobe, Japan.
authors.forename: Kenta
authors.initials: K
authors.lastname: Hara
authors.affiliation: Division of Diabetes and Metabolism, Department of Internal Medicine, Iwate Medical University School of Medicine, Morioka, Japan.
authors.forename: Kazuma
authors.initials: K
authors.lastname: Takahashi
authors.affiliation: Division of Internal Medicine and Diabetes, Kakogawa West City Hospital, Kakogawa, Japan.
authors.forename: Masao
authors.initials: M
authors.lastname: Nagata
authors.affiliation: Department of General Internal Medicine, Kobe University Graduate School of Medicine, Chuo-ku, Kobe, Japan.
authors.forename: Koichi
authors.initials: K
authors.lastname: Yokono
mesh_terms: D000818:Animals; D000938:Antigen-Presenting Cells / Q000276:immunology / Q000473:pathology; D016207:Cytokines / Q000276:immunology / Q000378:metabolism; D003713:Dendritic Cells / Q000276:immunology / Q000473:pathology; D003920:Diabetes Mellitus / Q000235:genetics* / Q000276:immunology / Q000473:pathology; D006801:Humans; D008264:Macrophages / Q000276:immunology / Q000473:pathology; D051379:Mice; D016688:Mice, Inbred NOD; D051117:Scavenger Receptors, Class A / Q000235:genetics*; D050378:T-Lymphocytes, Regulatory / Q000276:immunology / Q000378:metabolism* / Q000473:pathology
chemical_list: D016207:Cytokines; D051117:Scavenger Receptors, Class A