title: Modification interference analysis of the ribosome.
abstract: RNAs are versatile molecules involved in myriad functions in the cell. To understand how a RNA molecule functions in the cell it is important to identify the nucleotides in the RNA molecule that are important for its structure and function. There are several biochemical methods such as footprinting, cross-linking, and modification interference analysis that can be used to study RNA-RNA and RNA-protein interactions. Ribosome is a classical example of a RNA-protein complex that has been extensively studied using these methods. Here, we describe a modification interference method that was used to identify bases in 16S rRNA that are important for the translocation of the mRNA-tRNA complex by the ribosome.
authors.affiliation: Department of Chemistry and Biochemistry, University of California at San Diego, 4102 Urey Hall, 9500 Gilman Drive, La Jolla, CA, 92093-0314, USA, sjoseph@ucsd.edu.
authors.forename: Simpson
authors.initials: S
authors.lastname: Joseph
mesh_terms: D019921:Biotinylation; D003432:Cross-Linking Reagents / Q000378:metabolism; D004926:Escherichia coli / Q000378:metabolism; D008967:Molecular Biology / Q000379:methods*; D014176:Protein Biosynthesis; D012336:RNA, Ribosomal, 16S / Q000235:genetics / Q000378:metabolism; D012343:RNA, Transfer / Q000235:genetics; D012270:Ribosomes / Q000378:metabolism*
chemical_list: D003432:Cross-Linking Reagents; D012336:RNA, Ribosomal, 16S; D012343:RNA, Transfer