title: Native human papillomavirus production, quantification, and infectivity analysis.
abstract: In a natural infection, human papillomavirus (HPV) replicates in a stratified and differentiated epithelium. We have developed an in vitro organotypic raft culture system that allows researchers to study HPV in its natural environment. Not only does this system reproduce the differentiation-dependent replication cycle of HPV, but it also allows for the production of high titers of native HPV virions. Currently, much of the HPV research has been done utilizing synthetic particles produced in transfection systems. However, by production of native virions, this research can now be continued using native particles. This chapter presents methods for producing, titering, and qualitating, via infectivity assay, native virus produced from organotypic raft culture.
authors.affiliation: Department of Microbiology and Immunology, College of Medicine, The Pennsylvania State University, Mailbox H107, 500 University Drive, Hershey, PA, 17033, USA.
authors.forename: Jennifer
authors.initials: J
authors.lastname: Biryukov
authors.forename: Linda
authors.initials: L
authors.lastname: Cruz
authors.forename: Eric J
authors.initials: EJ
authors.lastname: Ryndock
authors.forename: Craig
authors.initials: C
authors.lastname: Meyers
mesh_terms: D000818:Animals; D002460:Cell Line; D004279:DNA, Viral / Q000302:isolation & purification; D006801:Humans; D051379:Mice; D027383:Papillomaviridae / Q000254:growth & development* / Q000472:pathogenicity*; D016133:Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014773:Virology / Q000379:methods*
chemical_list: D004279:DNA, Viral