title: Enzyme-catalyzed production of biodiesel by ultrasound-assisted ethanolysis of soybean oil in solvent-free system.
abstract: This work reports the transesterification of soybean oil with ethanol using a commercial immobilized lipase, Novozym 435, under the influence of ultrasound irradiation, in a solvent-free s. The experiments were performed in an ultrasonic water bath, following a sequence of experimental designs to evaluate the effects of temperature, enzyme and water concentrations, oil to ethanol molar ratio and output irradiation power on the reaction yield. Besides, a kinetic study varying the substrates molar ratio and enzyme concentration was also carried out. Results show that ultrasound-assisted lipase-catalyzed transesterification of soybean oil with ethanol in solvent-free system might be a potential alternative route to conventional alkali-catalyzed and/or traditional enzymatic methods, as high reaction yields (~78 wt%) were obtained at mild irradiation power supply (~132 W), and temperature (63 °C) in a relatively short reaction time, 1 h. Additionally, a study regarding the enzyme reuse was carried out at the experimental condition that afforded the best reaction yield.
authors.affiliation: Department of Food Engineering, URI-Campus de Erechim, Av. Sete de Setembro, 1621, Erechim, RS, 99700-000, Brazil.
authors.forename: Claudia M
authors.initials: CM
authors.lastname: Trentin
authors.forename: Ariana S
authors.initials: AS
authors.lastname: Popiolki
authors.forename: Luciane
authors.initials: L
authors.lastname: Batistella
authors.forename: Clarissa Dalla
authors.initials: CD
authors.lastname: Rosa
authors.forename: Helen
authors.initials: H
authors.lastname: Treichel
authors.forename: Débora
authors.initials: D
authors.lastname: de Oliveira
authors.forename: J Vladimir
authors.initials: JV
authors.lastname: Oliveira
mesh_terms: D056804:Biofuels; D002384:Catalysis; D004800:Enzymes, Immobilized; D005656:Fungal Proteins; D008049:Lipase / Q000737:chemistry*; D013016:Sound; D013024:Soybean Oil / Q000737:chemistry*
chemical_list: D056804:Biofuels; D004800:Enzymes, Immobilized; D005656:Fungal Proteins; D013024:Soybean Oil; C093667:Novozyme 435; D008049:Lipase