title: Differential inflammatory microRNA and cytokine expression in pulmonary sarcoidosis.
abstract: Sarcoidosis is a granulomatous disease of unknown etiology. The disease has an important inflammatory and immune component; however, its immunopathogenesis is not completely understood. Recently, the role of microRNAs (miRNAs), the small non-coding RNAs, has attracted attention as both being involved in pathogenesis and serving as disease markers. Accordingly, changes in the expression of some miRNAs have been also associated with different autoimmune pathologies. However, not much is known about the role of miRNAs in sarcoidosis. Therefore, the aim of this study was to compare the level of expression of selected miRNAs in healthy individuals and patients with sarcoidosis. We detected significantly increased level of miR-34a in peripheral blood mononuclear cells isolated from sarcoidosis patients. Moreover, significantly up-regulated levels of interferon (IFN)-γ, IFN-γ inducible protein (IP-10) and vascular endothelial growth factor were detected in sera of patients when compared to healthy subjects. Our results add to a known inflammatory component in sarcoidosis. Changes in the levels of miR-34a may suggest its involvement in the pathology of this disease.
authors.affiliation: Department of Medical Biotechnology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Gronostajowa 7, 30-387, Kraków, Poland.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Jazwa
authors.forename: Lukasz
authors.initials: L
authors.lastname: Kasper
authors.forename: Maciej
authors.initials: M
authors.lastname: Bak
authors.forename: Mateusz
authors.initials: M
authors.lastname: Sobczak
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Szade
authors.forename: Alicja
authors.initials: A
authors.lastname: Jozkowicz
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Sladek
authors.forename: Jozef
authors.initials: J
authors.lastname: Dulak
mesh_terms: D000328:Adult; D000368:Aged; D015551:Autoimmunity / Q000235:genetics; D054357:Chemokine CXCL10 / Q000097:blood; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D007249:Inflammation / Q000235:genetics; D007371:Interferon-gamma / Q000097:blood; D007963:Leukocytes, Mononuclear / Q000502:physiology*; D008297:Male; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D008875:Middle Aged; D017565:Sarcoidosis, Pulmonary / Q000235:genetics / Q000276:immunology*; D042461:Vascular Endothelial Growth Factor A / Q000097:blood
chemical_list: C114753:CXCL10 protein, human; D054357:Chemokine CXCL10; C522425:MIRN34 microRNA, human; D035683:MicroRNAs; D042461:Vascular Endothelial Growth Factor A; D007371:Interferon-gamma