title: Short communication: Anti-HIV-1 envelope immunoglobulin Gs in blood and cervicovaginal samples of Beninese commercial sex workers.
abstract: Characterization of the immune correlates of protection against HIV infection is crucial for the development of preventive strategies. This study examined HIV-1 envelope (Env) glycoproteins, specifically immunoglobulin G (IgG), in systemic and mucosal compartments of female Beninese commercial sex workers (CSWs). Samples of 23 HIV-1-positive and 20 highly exposed HIV-1-seronegative (HESN) CSWs were studied. HIV-1 Env-specific IgG detection in sera and cervicovaginal lavages (CVLs) from the study population was done by cell-based ELISA. The HIV neutralizing activity was evaluated with a neutralization assay. The HIV-1-specific antibody-dependent cellular cytotoxicity (ADCC) response of the cohort was measured with a FACS-based assay evaluating the ADCC-mediated elimination of gp120-coated target cells. No anti-HIV-1 Env-specific IgG neutralizing or ADCC activities were detected in samples from HESN CSWs. Samples from HIV-1-infected CSWs presented ADCC activity in both sera and CVLs. Anti-Env IgG from sera and CVLs from HIV-1-infected CSWs preferentially recognized Env in its CD4-bound conformation. HIV-1-infected CSWs have ADCC-mediating IgG that preferentially recognizes Env in its CD4-bound conformation at the mucosal site.
authors.affiliation: 1 Centre de Recherche du Centre Hospitalier de l'Université de Montréal , Montréal, Québec, Canada .
authors.forename: Laurie-Anne
authors.initials: LA
authors.lastname: Batraville
authors.forename: Jonathan
authors.initials: J
authors.lastname: Richard
authors.forename: Maxime
authors.initials: M
authors.lastname: Veillette
authors.forename: Annie-Claude
authors.initials: AC
authors.lastname: Labbé
authors.forename: Michel
authors.initials: M
authors.lastname: Alary
authors.forename: Fernand
authors.initials: F
authors.lastname: Guédou
authors.forename: Daniel E
authors.initials: DE
authors.lastname: Kaufmann
authors.forename: Johanne
authors.initials: J
authors.lastname: Poudrier
authors.forename: Andrés
authors.initials: A
authors.lastname: Finzi
authors.forename: Michel
authors.initials: M
authors.lastname: Roger
mesh_terms: D000293:Adolescent; D000328:Adult; D000920:Antibody-Dependent Cell Cytotoxicity; D001541:Benin; D012634:Bodily Secretions / Q000276:immunology*; D003142:Communication; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005836:Genitalia, Female / Q000276:immunology*; D015483:HIV Antibodies / Q000032:analysis* / Q000097:blood*; D015658:HIV Infections / Q000276:immunology*; D015497:HIV-1 / Q000276:immunology*; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis / Q000097:blood; D007136:Immunoglobulins; D009500:Neutralization Tests; D011477:Sex Work; D060386:Sex Workers; D044364:Vaginal Douching; D055815:Young Adult; D054299:env Gene Products, Human Immunodeficiency Virus / Q000276:immunology
chemical_list: D015483:HIV Antibodies; D007074:Immunoglobulin G; D007136:Immunoglobulins; D054299:env Gene Products, Human Immunodeficiency Virus