title: Two novel exopolysaccharides from Bacillus amyloliquefaciens C-1: antioxidation and effect on oxidative stress.
abstract: Two odorless, water-soluble exopolysaccharide (EPS) fractions, EPS-1 and EPS-2, were isolated from a newly isolated Bacillus amyloliquefaciens strain C-1 and purified by ion exchange and gel chromatography. The purified EPS-1 contained glucose/mannose/galactose/arabinose in a relative proportion of 15:4:2:1, and possessed a molecular weight of 79.6 kDa, while EPS-2 contained only glucose and mannose in a 3:1 ratio, with the molecular weights of 19.8 kDa. The antioxidant activity results showed that EPS-1 exhibited strong reducing power, superoxide radicals (O(2-)·), and hydroxyl free radicals (OH·) scavenging activities. For the H2O2-induced injury in HepG2 cells, EPS-1 significantly decreased the formation of reactive oxygen species, intracellular malondialdehyde levels, and restored intracellular superoxide dismutase activity. For EPS-2, there had no detectable antioxidant activities. And all these results collectively showed that as a natural antioxidant, only EPS-1 produced by C-1 had considerable potential to be used as medical compounds or functional additives.
authors.affiliation: Health Science Center, Xi'an Jiaotong University, P.O. 44, #76 Yanta West Road, Xi'an, 710061, Shaanxi, China.
authors.forename: Haixia
authors.initials: H
authors.lastname: Yang
authors.forename: Jianjun
authors.initials: J
authors.lastname: Deng
authors.forename: Yue
authors.initials: Y
authors.lastname: Yuan
authors.forename: Daidi
authors.initials: D
authors.lastname: Fan
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.forename: Ruijuan
authors.initials: R
authors.lastname: Zhang
authors.forename: Bei
authors.initials: B
authors.lastname: Han
mesh_terms: D000975:Antioxidants / Q000302:isolation & purification / Q000494:pharmacology; D001407:Bacillus / Q000145:classification / Q000235:genetics / Q000254:growth & development / Q000378:metabolism* / Q000648:ultrastructure; D005285:Fermentation; D016166:Free Radical Scavengers / Q000494:pharmacology; D056945:Hep G2 Cells; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism; D008315:Malondialdehyde / Q000378:metabolism; D008970:Molecular Weight; D010084:Oxidation-Reduction / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects; D010802:Phylogeny; D011135:Polysaccharides, Bacterial / Q000096:biosynthesis / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017382:Reactive Oxygen Species / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D000975:Antioxidants; D016166:Free Radical Scavengers; D011135:Polysaccharides, Bacterial; D012336:RNA, Ribosomal, 16S; D017382:Reactive Oxygen Species; C085953:exopolysaccharide, Bacillus; D008315:Malondialdehyde; D006861:Hydrogen Peroxide; D013482:Superoxide Dismutase