title: An integrated microspectrometer for localised multiplexing measurements.
abstract: We describe the development of an integrated lensed Arrayed Waveguide Grating (AWG) microspectrometer for localized multiplexing fluorescence measurements. The device, which has a footprint that is only 1 mm wide and 1 cm long, is capable of spectroscopic measurements on chip. Multiple fluorescence signals were measured simultaneously based upon simple intensity readouts from a CCD camera. We also demonstrate the integration of the AWG spectrometer with a microfluidic platform using a lensing function to confine the beam shape for focused illumination. This capability enhances signal collection, gives better spatial resolution, and provides a route for the analysis of small volume samples (e.g. cells) in flow. To show these capabilities we developed a novel "bead-AWG" platform with which we demonstrate localized multiplexed fluorescence detection either simultaneously or successively. Such an integrated system provides the basis for a portable system capable of optical detection of multi-wavelength fluorescence from a single defined location.
authors.affiliation: Division of Biomedical Engineering, School of Engineering, University of Glasgow, Glasgow, UK. huzhixiong@nim.ac.cn huabing.yin@glasgow.ac.uk.
authors.forename: Zhixiong
authors.initials: Z
authors.lastname: Hu
authors.forename: Andrew
authors.initials: A
authors.lastname: Glidle
authors.forename: Charles
authors.initials: C
authors.lastname: Ironside
authors.forename: Jonathan M
authors.initials: JM
authors.lastname: Cooper
authors.forename: Huabing
authors.initials: H
authors.lastname: Yin
mesh_terms: D004867:Equipment Design; D005456:Fluorescent Dyes; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods; D008863:Microspheres; D045663:Quantum Dots; D013050:Spectrometry, Fluorescence / Q000295:instrumentation* / Q000379:methods; D019809:Streptavidin
chemical_list: D005456:Fluorescent Dyes; D019809:Streptavidin