title: Effects of elongation delay in transcription dynamics.
abstract: In the transcription process, elongation delay is induced by the movement of RNA polymerases (RNAP) along the DNA sequence, and can result in changes in the transcription dynamics. This paper studies the transcription dynamics that involved the elongation delay and effects of cell division and DNA replication. The stochastic process of gene expression is modeled with delay chemical master equation with periodic coefficients, and is studied numerically through the stochastic simulation algorithm with delay. We show that the average transcription level approaches to a periodic dynamics over cell cycles at homeostasis, and the elongation delay can reduce the transcription level and increase the transcription noise. Moreover, the transcription elongation can induce bimodal distribution of mRNA levels that can be measured by the techniques of flow cytometry.
authors.affiliation: School of Mathematics and Systems Science, Beihang University, Beijing 100191, China. zhangxuan_66@yeah.net.
authors.forename: Xuan
authors.initials: X
authors.lastname: Zhang
authors.forename: Huiqin
authors.initials: H
authors.lastname: Jin
authors.forename: Zhuoqin
authors.initials: Z
authors.lastname: Yang
authors.forename: Jinzhi
authors.initials: J
authors.lastname: Lei
mesh_terms: D000465:Algorithms; D002453:Cell Cycle / Q000235:genetics*; D003198:Computer Simulation; D004247:DNA / Q000235:genetics*; D004261:DNA Replication / Q000235:genetics*; D012321:DNA-Directed RNA Polymerases / Q000235:genetics*; D008957:Models, Genetic; D012333:RNA, Messenger / Q000235:genetics*; D013269:Stochastic Processes; D014158:Transcription, Genetic
chemical_list: D012333:RNA, Messenger; D004247:DNA; D012321:DNA-Directed RNA Polymerases