title: Dermal dendritic cells, but not Langerhans cells, are critical in murine single epicutaneous sensitization.
abstract: A murine repeated protein-patch model has been established to study epicutaneous sensitization in atopic dermatitis. This model has shown a predominant Th2 and a weak Th1 response in both BALB/c and C57BL/6 mice. However, Th responses induced in the repeated model are not consistent with the generally accepted theory that BALB/c and C57BL/6 mice are Th2 and Th1 prone and are representatives of human atopy and non-atopy, respectively. In this study, a single protein-patch model was established, which showed in addition to the Th2 response, a remarkable Th1 response in C57BL/6 mice, but not in BALB/c mice. Moreover, using muLangerin-DTR mice, we demonstrated that dermal dendritic cells, but not Langerhans cells, are critical in single epicutaneous sensitization in both strains of mice.
authors.affiliation: Department of Dermatology, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine, Kaohsiung, Taiwan.
authors.forename: Chih-Hung
authors.initials: CH
authors.lastname: Lee
authors.forename: Jau-Shiuh
authors.initials: JS
authors.lastname: Chen
authors.forename: Hsien-Ching
authors.initials: HC
authors.lastname: Chiu
authors.forename: Chien-Hui
authors.initials: CH
authors.lastname: Hong
authors.forename: Ching-Yi
authors.initials: CY
authors.lastname: Liu
authors.forename: Yng-Cun
authors.initials: YC
authors.lastname: Ta
authors.forename: Bjőrn E
authors.initials: BE
authors.lastname: Clausen
authors.forename: Ji-Chen
authors.initials: JC
authors.lastname: Ho
authors.forename: Li-Fang
authors.initials: LF
authors.lastname: Wang
mesh_terms: D000485:Allergens / Q000276:immunology; D000818:Animals; D015703:Antigens, CD / Q000378:metabolism; D002465:Cell Movement; D002478:Cells, Cultured; D016207:Cytokines / Q000276:immunology; D003713:Dendritic Cells / Q000166:cytology*; D003876:Dermatitis, Atopic / Q000276:immunology; D006801:Humans; D039001:Integrin alpha Chains / Q000378:metabolism; D007801:Langerhans Cells / Q000166:cytology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D012867:Skin / Q000276:immunology* / Q000473:pathology; D018417:Th1 Cells / Q000166:cytology; D018418:Th2 Cells / Q000166:cytology
chemical_list: D000485:Allergens; D015703:Antigens, CD; D016207:Cytokines; D039001:Integrin alpha Chains; C091367:alpha E integrins
keywords: Th1/Th2; dendritic cell subset; epicutaneous sensitization