title: Selected ⁶⁸Ga-siderophores versus ⁶⁸Ga-colloid and ⁶⁸Ga-citrate: biodistribution and small animal imaging in mice.
abstract: BACKGROUND
  (68)Ga-triacetylfusarinine C (TAFC) and (68)Ga-ferrioxamine E (FOXE) show great potential to be used as highly sensitive and selective tracers for Aspergillus infection imaging. Here we report on a comparison of the ex vivo biodistribution and small animal imaging of (68)Ga-TAFC and (68)Ga-FOXE versus (68)Ga-colloid and (68)Ga-citrate as unspecific control in mice.
  
  
  METHODS
  The radiochemical purity of tested (68)Ga labelled tracers was determined by RP-HPLC or ITLC-SG. Ex vivo biodistribution was studied in normal DBA/2 mice 30 min and 90 min p.i. Static and dynamic imaging were performed using µPET/CT.
  
  
  RESULTS
  (68)Ga-TAFC and (68)Ga-FOXE showed rapid renal excretion and low blood values even 90 min p.i. (68)Ga-TAFC showed almost no retention in other organs while (68)Ga-FOXE displayed some uptake in gastrointestinal tract. (68)Ga-colloid and (68)Ga-citrate revealed significantly different ex vivo biodistribution. (68)Ga-colloid showed pronounced radioactivity retention in the liver, while (68)Ga-citrate displayed high blood values and significant retention of radioactivity in highly perfused organs.
  
  
  CONCLUSIONS
  From the results, both (68)Ga-TAFC and (68)Ga-FOXE have excellent and significantly different in vivo behaviour compared to (68)Ga-colloid and (68)Ga-citrate. (68)Ga-TAFC in particular confirmed its great potential use as a specific tracer for Aspergillus infection imaging.
authors.affiliation: Institute of Molecular and Translational Medicine, Faculty of Medicine and Dentistry, Palacky University Olomouc, Czech Republic.
authors.forename: Milos
authors.initials: M
authors.lastname: Petrik
authors.forename: Adela
authors.initials: A
authors.lastname: Vlckova
authors.forename: Zbynek
authors.initials: Z
authors.lastname: Novy
authors.forename: Lubor
authors.initials: L
authors.lastname: Urbanek
authors.forename: Hubertus
authors.initials: H
authors.lastname: Haas
authors.forename: Clemens
authors.initials: C
authors.lastname: Decristoforo
mesh_terms: D000818:Animals; D001228:Aspergillosis / Q000000981:diagnostic imaging* / Q000378:metabolism; D002951:Citrates / Q000493:pharmacokinetics*; D003102:Colloids; D003952:Diagnostic Imaging; D005708:Gallium / Q000493:pharmacokinetics*; D005710:Gallium Radioisotopes; D006573:Heterocyclic Compounds, 1-Ring / Q000493:pharmacokinetics*; D051379:Mice; D008811:Mice, Inbred DBA; D010456:Peptides, Cyclic / Q000493:pharmacokinetics*; D049268:Positron-Emission Tomography / Q000379:methods*; D019275:Radiopharmaceuticals / Q000493:pharmacokinetics; D017262:Siderophores; D013004:Somatostatin / Q000031:analogs & derivatives* / Q000493:pharmacokinetics; D014018:Tissue Distribution
chemical_list: D002951:Citrates; D003102:Colloids; D005710:Gallium Radioisotopes; D006573:Heterocyclic Compounds, 1-Ring; D010456:Peptides, Cyclic; D019275:Radiopharmaceuticals; D017262:Siderophores; C558148:gallium 68-DOTA-lanreotide; D013004:Somatostatin; D005708:Gallium; C103850:gallium citrate