title: Recruitment of bone marrow-derived cells to periodontal tissue defects.
abstract: Bone marrow-derived cells (BMCs) are considered to be a major source of mesenchymal stem cells (MSCs) in adults and are known to be effective in periodontal tissue regeneration. However, whether endogenous BMCs are involved in periodontal tissue repair process is uncertain. We therefore created periodontal tissue defects in the buccal alveolar bone of mandibular first molars in bone marrow chimeric mice, and immunohistochemically examined the expression of stromal cell derived factor-1 (SDF-1) and the mobilization of BMCs. We found that SDF-1 expression was increased around the defects at as early as 1 week after injury and that BMCs were mobilized to the defects, while GFP+/CD45+ were rarely observed. Fluorescence-activated cell sorting (FACS) analysis demonstrated that the number of platelet-derived growth factor receptor (pdgfr) α+/Sca-1+ (PαS) cells in the bone marrow decreased after injury. Taken together, these results suggest that BMCs are mobilized to the periodontal tissue defects. Recruitment of BMCs, including a subset of MSCs could be a new target of periodontal treatment.
authors.affiliation: Department of Periodontology, Graduate School of Medical and Dental Science, Tokyo Medical and Dental University Tokyo, Japan.
authors.forename: Yasuyuki
authors.initials: Y
authors.lastname: Kimura
authors.affiliation: Department of Nanomedicine (DNP), Graduate School of Medical and Dental Science, Tokyo Medical and Dental University Tokyo, Japan.
authors.forename: Motohiro
authors.initials: M
authors.lastname: Komaki
authors.affiliation: Department of Nanomedicine (DNP), Graduate School of Medical and Dental Science, Tokyo Medical and Dental University Tokyo, Japan.
authors.forename: Kengo
authors.initials: K
authors.lastname: Iwasaki
authors.affiliation: Department of Cardiovascular Medicine, Institute of Health Biosciences, The University of Tokushima Graduate School Tokushima, Japan.
authors.forename: Masataka
authors.initials: M
authors.lastname: Sata
authors.affiliation: Department of Periodontology, Graduate School of Medical and Dental Science, Tokyo Medical and Dental University Tokyo, Japan ; Global Center of Excellence Program, International Research Center for Molecular Science in Tooth and Bone Diseases, Tokyo Medical and Dental University Tokyo, Japan.
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Izumi
authors.affiliation: Global Center of Excellence Program, International Research Center for Molecular Science in Tooth and Bone Diseases, Tokyo Medical and Dental University Tokyo, Japan ; Department of Cellular Physiological Chemistry, Graduate School of Medical and Dental Science, Tokyo Medical and Dental University Tokyo, Japan.
authors.forename: Ikuo
authors.initials: I
authors.lastname: Morita
keywords: SDF-1; bone marrow chimeric mice; mesenchymal stem cells; periodontal defects; recruitment