title: Fast and Simultaneous Analysis of Combined Anti-Diabetic Drugs by Capillary Zone Electrophoresis.
abstract: A fast capillary zone electrophoretic method with photodiode array detection (CZE-PAD) was established and validated for assays of commonly prescribed anti-diabetic drugs [metformin (MET), glibenclamide (GBM) and gliclazide (GCZ)] in 13 samples including raw material, single and combined tablets. CZE optimization revealed baseline separation of the analytes (Rs > 5.39) in 8 min, in 50 mM borate buffer (pH 9.0), using a capillary with an effective length of 56.0 cm and an inner diameter of 50 µm, a voltage of 20 kV, a temperature of 25°C and a detection wavelength at 210 nm. The method provides excellent linearity, precision (%RSDs < 1.90%), recovery (99.8-101.0%) and low detection and quantitation limits (<4 and 12 µg/mL, respectively). The procedure was fast (seven samples per hour) and cost effective, since no organic solvent, sample pre-treatments or clean-up procedures were required. Importantly, the method was accurate, sensitive and reliable for routine quality control of MET, GBM and GCZ in pharmaceutical products both in single and combined formulations.
authors.affiliation: Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Mahidol University, Bangkok, Thailand.
authors.forename: Athiporn
authors.initials: A
authors.lastname: Doomkaew
authors.affiliation: Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Mahidol University, Bangkok, Thailand.
authors.forename: Pornpan
authors.initials: P
authors.lastname: Prapatpong
authors.affiliation: Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Mahidol University, Bangkok, Thailand.
authors.forename: Sawanya
authors.initials: S
authors.lastname: Buranphalin
authors.affiliation: Department of Analytical Chemistry and Pharmaceutical Technology, Center for Pharmaceutical Research (CePhaR), Vrije Universiteit Brussel, Brussels 1090, Belgium.
authors.forename: Yvan
authors.initials: Y
authors.lastname: Vander Heyden
authors.affiliation: Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Mahidol University, Bangkok, Thailand Center of Excellence for Innovation in Drug Design and Discovery, Faculty of Pharmacy, Mahidol University, Bangkok, Thailand leena.sun@mahidol.ac.th.
authors.forename: Leena
authors.initials: L
authors.lastname: Suntornsuk
mesh_terms: D004338:Drug Combinations; D019075:Electrophoresis, Capillary / Q000379:methods; D005907:Gliclazide / Q000032:analysis* / Q000737:chemistry; D005905:Glyburide / Q000032:analysis* / Q000737:chemistry; D007004:Hypoglycemic Agents / Q000032:analysis* / Q000737:chemistry; D057230:Limit of Detection; D016014:Linear Models; D008687:Metformin / Q000032:analysis* / Q000737:chemistry; D015203:Reproducibility of Results; D013607:Tablets
chemical_list: D004338:Drug Combinations; D007004:Hypoglycemic Agents; D013607:Tablets; D008687:Metformin; D005907:Gliclazide; D005905:Glyburide