title: Inducing effect of clofibric acid on stearoyl-CoA desaturase in intestinal mucosa of rats.
abstract: Fibrates have been reported to elevate the hepatic proportion of oleic acid (18:1n-9) through inducing stearoyl-CoA desaturase (SCD). Despite abundant studies on the regulation of SCD in the liver, little is known about this issue in the small intestine. The present study aimed to investigate the effect of clofibric acid on the fatty acid profile, particularly monounsaturated fatty acids (MUFA), and the SCD expression in intestinal mucosa. Treatment of rats with a diet containing 0.5% (w/w) clofibric acid for 7 days changed the MUFA profile of total lipids in intestinal mucosa; the proportion of 18:1n-9 was significantly increased, whereas those of palmitoleic (16:1n-7) and cis-vaccenic (18:1n-7) acids were not changed. Upon the treatment with clofibric acid, SCD was induced and the gene expression of SCD1, SCD2, and fatty acid elongase (Elovl) 6 was up-regulated, but that of Elovl5 was unaffected. Fat-free diet feeding for 28 days increased the proportions of 16:1n-7 and 18:1n-7, but did not effectively change that of 18:1n-9, in intestinal mucosa. Fat-free diet feeding up-regulated the gene expression of SCD1, but not that of SCD2, Elovl6, or Elovl5. These results indicate that intestinal mucosa significantly changes its MUFA profile in response to challenges by clofibric acid and a fat-free diet and suggest that up-regulation of the gene expression of SCD along with Elovl6 is indispensable to elevate the proportion of 18:1n-9 in intestinal mucosa.
authors.affiliation: School of Pharmaceutical Sciences, Josai University, 1-1 Keyakidai, Sakado, Saitama, 350-0295, Japan.
authors.forename: Tohru
authors.initials: T
authors.lastname: Yamazaki
authors.forename: Makiko
authors.initials: M
authors.lastname: Kadokura
authors.forename: Yuki
authors.initials: Y
authors.lastname: Mutoh
authors.forename: Takeshi
authors.initials: T
authors.lastname: Sakamoto
authors.forename: Mari
authors.initials: M
authors.lastname: Okazaki
authors.forename: Atsushi
authors.initials: A
authors.lastname: Mitsumoto
authors.forename: Yoichi
authors.initials: Y
authors.lastname: Kawashima
authors.forename: Naomi
authors.initials: N
authors.lastname: Kudo
mesh_terms: D042962:Acyl-CoA Oxidase; D000818:Animals; D002995:Clofibric Acid / Q000494:pharmacology*; D018752:Diet, Fat-Restricted; D005227:Fatty Acids / Q000378:metabolism; D005229:Fatty Acids, Monounsaturated / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D007413:Intestinal Mucosa / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism*; D050356:Lipid Metabolism / Q000187:drug effects; D008055:Lipids / Q000737:chemistry; D008297:Male; D010088:Oxidoreductases / Q000235:genetics; D047493:PPAR alpha / Q000235:genetics; D017208:Rats, Wistar; D013230:Stearoyl-CoA Desaturase / Q000235:genetics / Q000378:metabolism*; D051780:Sterol Regulatory Element Binding Protein 1 / Q000235:genetics
chemical_list: D005227:Fatty Acids; D005229:Fatty Acids, Monounsaturated; D008055:Lipids; D047493:PPAR alpha; D051780:Sterol Regulatory Element Binding Protein 1; D002995:Clofibric Acid; D010088:Oxidoreductases; D013230:Stearoyl-CoA Desaturase; C107176:peroxisomal acyl-CoA oxidase; D042962:Acyl-CoA Oxidase