title: Identification of Mycoplasma suis MSG1 interaction proteins on porcine erythrocytes.
abstract: Adhesion protein MSG1 mediating adherence to porcine erythrocytes in Mycoplasma suis (M. suis) invasion has been identified previously. In order to determine the host membrane proteins that interact with MSG1, recombinant His-tagged MSG1 (rMSG1) was used to screen for interacting proteins in the protein extracts of porcine erythrocyte membrane. Potential rMSG1-interacting proteins were initially identified as band 3 and β-actin with molecular weight of 46 and 45 kDa, respectively. Immune fluorescence results showed that rMSG1 can specifically bind with the β-actin of HeLa, BHK-21, and HEK-293A cells, respectively. RNA interference assays further demonstrated that the interaction between β-actin and rMSG1 on HeLa cells was specific and dose dependent. Confocal microscopy showed that both rMSG1 and M. suis can partially co-localize with β-actin on the surface of porcine erythrocytes. Pull-down assays showed that rMSG1 can directly interact with β-actin. Our study is the first to report the interaction of MSG1 with β-actin, which will be of help to understand the pathogenesis of M. suis and develop a cultivation system.
authors.affiliation: Key Laboratory of Bacteriology, Ministry of Agriculture, College of Veterinary Medicine, Nanjing Agricultural University, 210095, Nanjing, China.
authors.forename: Yaning
authors.initials: Y
authors.lastname: Zhang
authors.forename: Yao
authors.initials: Y
authors.lastname: Zou
authors.forename: Peipei
authors.initials: P
authors.lastname: Ma
authors.forename: Hassan Mushtaq
authors.initials: HM
authors.lastname: Muhammad
authors.forename: Yufeng
authors.initials: Y
authors.lastname: Li
authors.forename: Ping
authors.initials: P
authors.lastname: Jiang
mesh_terms: D000199:Actins / Q000378:metabolism; D000818:Animals; D001426:Bacterial Proteins / Q000378:metabolism*; D002462:Cell Membrane / Q000382:microbiology; D004912:Erythrocytes / Q000382:microbiology*; D057809:HEK293 Cells; D006367:HeLa Cells; D006801:Humans; D008565:Membrane Proteins / Q000378:metabolism; D018613:Microscopy, Confocal; D009174:Mycoplasma / Q000378:metabolism; D009175:Mycoplasma Infections / Q000382:microbiology / Q000662:veterinary*; D011485:Protein Binding; D021381:Protein Transport; D011994:Recombinant Proteins / Q000378:metabolism; D013552:Swine; D013553:Swine Diseases / Q000382:microbiology*; D015534:Trans-Activators / Q000378:metabolism
chemical_list: D000199:Actins; D001426:Bacterial Proteins; D008565:Membrane Proteins; D011994:Recombinant Proteins; D015534:Trans-Activators