title: Acute restraint stress impairs induction of long-term potentiation by activating GSK-3β.
abstract: The present study investigated the effect of acute restraint stress on long-term potentiation (LTP) and the underlying mechanism. Induction of Schaffer collateral-CA1 LTP was suppressed in hippocampal slices from mice with 1-h restraint stress. Cell surface localization of the N-methyl-D-aspartate (NMDA) receptor subunits NR1 and NR2B and the α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptor subunits GluA1 and GluA2 was not affected in the hippocampus from mice with 1-h restraint stress. Phosphorylation of Akt at Ser473, but not at Thr308, and phosphorylation of GSK-3β at Ser9, but not at Tyr216, were significantly inhibited in the hippocampus with 1-h restraint stress. Taken together, the results of the present study show that acute restraint stress impairs induction of LTP by enhancing GSK-3β activity following suppressed Akt activity, without affecting cell surface localization of the NMDA and AMPA receptor subunits.
authors.affiliation: Division of Bioinformation, Department of Physiology, Hyogo College of Medicine, 1-1 Mukogawa-cho, Nishinomiya, 663-8501, Japan.
authors.forename: Yu
authors.initials: Y
authors.lastname: Jin
authors.forename: Takeshi
authors.initials: T
authors.lastname: Kanno
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Nishizaki
mesh_terms: D000818:Animals; D004789:Enzyme Activation; D038362:Glycogen Synthase Kinase 3 / Q000378:metabolism*; D000071679:Glycogen Synthase Kinase 3 beta; D006624:Hippocampus / Q000201:enzymology / Q000378:metabolism; D017774:Long-Term Potentiation; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D017382:Reactive Oxygen Species / Q000378:metabolism; D018091:Receptors, AMPA / Q000187:drug effects; D013315:Stress, Psychological / Q000201:enzymology / Q000378:metabolism*
chemical_list: D017382:Reactive Oxygen Species; D018091:Receptors, AMPA; D000071679:Glycogen Synthase Kinase 3 beta; C000605507:Gsk3b protein, mouse; D038362:Glycogen Synthase Kinase 3