title: Energetically biased DNA motor containing a thermodynamically stable partial strand displacement state.
abstract: Current work in tuning DNA kinetics has focused on changing toehold lengths and DNA concentrations. However, kinetics can also be improved by enhancing the completion probability of the strand displacement process. Here, we execute this strategy by creating a toehold DNA motor device with the inclusion of a synthetic nucleotide, inosine, at selected sites. Furthermore, we found that the energetic bias can be tuned such that the device can stay in a stable partially displaced state. This work demonstrates the utility of energetic biases to change DNA strand displacement kinetics and introduces a complementary strategy to the existing designs.
authors.affiliation: Department of Bioengineering, ‡Department of Mechanical and Aerospace Engineering, §Materials Science and Engineering, and ∥Department of Nanoengineering, University of California, San Diego , 9500 Gilman Drive, La Jolla, California 92093, United States.
authors.forename: Preston B
authors.initials: PB
authors.lastname: Landon
authors.forename: Joon
authors.initials: J
authors.lastname: Lee
authors.forename: Michael Taeyoung
authors.initials: MT
authors.lastname: Hwang
authors.forename: Alexander H
authors.initials: AH
authors.lastname: Mo
authors.forename: Chen
authors.initials: C
authors.lastname: Zhang
authors.forename: Anthony
authors.initials: A
authors.lastname: Neuberger
authors.forename: Brian
authors.initials: B
authors.lastname: Meckes
authors.forename: Jose J
authors.initials: JJ
authors.lastname: Gutierrez
authors.forename: Gennadi
authors.initials: G
authors.lastname: Glinsky
authors.forename: Ratnesh
authors.initials: R
authors.lastname: Lal
mesh_terms: D004247:DNA / Q000737:chemistry*; D007700:Kinetics
chemical_list: D004247:DNA