title: Measurement of deubiquitinating enzyme activity via a suicidal HA-Ub-VS probe.
abstract: Deubiquitinating enzymes (DUBs) are a novel "drug-able" target for cervical cancer, and small-molecule inhibitors of DUBs are currently being evaluated as novel chemotherapeutic agents. In this chapter, we describe an enzyme activity assay to assess the selectivity of a putative small-molecule DUB inhibitor toward a subset of DUBs in cancer cell lines.
authors.affiliation: Department of Obstetrics, University of Minnesota Medical School, MMC 395 Mayo, 8395C (Campus Delivery Code), 420 Delaware Street SE, Minneapolis, MN, 55455, USA, clrivard@umn.edu.
authors.forename: Colleen
authors.initials: C
authors.lastname: Rivard
authors.forename: Martina
authors.initials: M
authors.lastname: Bazzaro
mesh_terms: D045744:Cell Line, Tumor; D057075:Enzyme Assays / Q000379:methods*; D005260:Female; D041722:Genes, Transgenic, Suicide; D006388:Hemagglutinins / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D015335:Molecular Probes / Q000378:metabolism*; D017434:Protein Structure, Tertiary; D013450:Sulfones / Q000378:metabolism*; D025801:Ubiquitin / Q000378:metabolism*; D064570:Ubiquitin-Specific Proteases / Q000378:metabolism*
chemical_list: D006388:Hemagglutinins; D015335:Molecular Probes; D013450:Sulfones; D025801:Ubiquitin; C060916:methyl vinyl sulfone; D064570:Ubiquitin-Specific Proteases