title: Human procollagen type I surface-modified PHB-based non-woven textile scaffolds for cell growth: preparation and short-term biological tests.
abstract: 3D fine porous structures obtained by electrospinning a poly[(R,S)-3-hydroxybutyrate] (aPHB)/ poly[(R)-3-hydroxybutyrate] (PHB) (85/15 w/w) blend were successfully modified with human procollagen type I by simple immersion of the polyester scaffold in an aqueous solution of the protein. Effective modification of the scaffold with human procollagen I was confirmed by an immunodetection test, which revealed the presence of the procollagen type I as an outer layer even on inner structures of the porous matrixes. Biological tests of 3D fabrics made of the PHB blend provide support for the adhesion and proliferation of human fibroblasts, while their modification with procollagen type I increased the biocompatibility of the final scaffolds significantly, as shown by the notable increase in the number of attached cells during the early hours of their incubation. Based on these findings, human procollagen type I surface-modified aPHB/PHB scaffolds should be considered a promising material in regenerative medicine.
authors.affiliation: Polish Academy of Sciences, Centre of Polymer and Carbon Materials, 34, M. Curie-Skłodowska St., 41-819 Zabrze, Poland.
authors.forename: Michał
authors.initials: M
authors.lastname: Kawalec
authors.forename: Anna
authors.initials: A
authors.lastname: Sitkowska
authors.forename: Michał
authors.initials: M
authors.lastname: Sobota
authors.forename: Aleksander L
authors.initials: AL
authors.lastname: Sieroń
authors.forename: Patrycja
authors.initials: P
authors.lastname: Komar
authors.forename: Piotr
authors.initials: P
authors.lastname: Kurcok
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry*; D002448:Cell Adhesion; D049109:Cell Proliferation; D002478:Cells, Cultured; D024042:Collagen Type I / Q000737:chemistry*; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism; D006801:Humans; D006885:Hydroxybutyrates / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D010455:Peptides / Q000737:chemistry; D011091:Polyesters / Q000737:chemistry; D016062:Porosity; D000091402:Prohibitins; D012038:Regeneration; D012492:Salts / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared; D013499:Surface Properties; D023822:Tissue Engineering / Q000379:methods; D054457:Tissue Scaffolds / Q000737:chemistry*; D014783:Viscosity
chemical_list: D001672:Biocompatible Materials; D024042:Collagen Type I; D006885:Hydroxybutyrates; C000717328:PHB protein, human; D010455:Peptides; D011091:Polyesters; D000091402:Prohibitins; D012492:Salts