title: Laser desorption postionization mass spectrometry imaging of biological targets.
abstract: Laser desorption photoionization mass spectrometry (LDPI-MS) utilizes two separate light sources for desorption and photoionization of species from a solid surface. This technique has been applied to study a wide variety of molecular analytes in biological systems, but is not yet available in commercial instruments. For this reason, a generalized protocol is presented here for the use of LDPI-MS imaging to detect small molecules within intact biological samples. Examples are provided here for LDPI-MS imaging of an antibiotic within a tooth root canal and a metabolite within a coculture bacterial biofilm.
authors.affiliation: Department of Chemistry, University of Illinois at Chicago, MC 111, 845 W. Taylor St., 4500 SES, Chicago, IL, 60607-7061, USA.
authors.forename: Artem
authors.initials: A
authors.lastname: Akhmetov
authors.forename: Chhavi
authors.initials: C
authors.lastname: Bhardwaj
authors.forename: Luke
authors.initials: L
authors.lastname: Hanley
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D018441:Biofilms / Q000254:growth & development; D002417:Cattle; D003786:Dental Pulp Cavity / Q000187:drug effects; D004318:Doxycycline / Q000494:pharmacology; D004926:Escherichia coli / Q000378:metabolism / Q000502:physiology; D057054:Molecular Imaging / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
chemical_list: D000900:Anti-Bacterial Agents; D004318:Doxycycline