title: Fluorescence anisotropy: analysis of tRNA binding to the T box riboswitch antiterminator RNA.
abstract: Fluorescence anisotropy can be utilized in drug discovery screening assays to identify compounds that disrupt medicinally important RNA-macromolecular complexes. Here we describe the application of this technique to monitor tRNA binding to T box riboswitch antiterminator RNA.
authors.affiliation: Department of Chemistry and Biochemistry, Ohio University, Athens, OH, 45701, USA.
authors.forename: S
authors.initials: S
authors.lastname: Zhou
authors.forename: R
authors.initials: R
authors.lastname: Anupam
authors.forename: J V
authors.initials: JV
authors.lastname: Hines
mesh_terms: D001412:Bacillus subtilis / Q000378:metabolism; D001483:Base Sequence; D003956:Dialysis; D005454:Fluorescence Polarization / Q000379:methods*; D008969:Molecular Sequence Data; D012329:RNA, Bacterial / Q000235:genetics / Q000378:metabolism*; D012343:RNA, Transfer / Q000235:genetics / Q000378:metabolism*; D058928:Riboswitch; D013728:Terminator Regions, Genetic
chemical_list: D012329:RNA, Bacterial; D058928:Riboswitch; D012343:RNA, Transfer