title: Interaction of six candidate genes in essential hypertension.
abstract: We explored the interaction of 6 candidate genetic mutations in essential hypertension (EH). The mutations AGT M235T, ACE I/D, eNOS Glu298Asp, ET-2 A985G, ANP T2238C, and NPRC A-55C were detected using a genechip microarray in 100 patients with EH and 97 controls from the Han population living in the Yunnan Province of China. Risks of EH were evaluated with respect to a combination of these genotypes. Interactions were analyzed using multifactor dimensionality reduction (MDR). P values were corrected using Bonferroni's adjustment. Results showed that CC genotype frequencies for NPRC A-55C (0.540) in EH were significantly higher than those in controls (0.237, Pc < 0.01; odds ratio (OR) = 3.777; 95% confidence interval (CI) = 2.050-6.960). The OR for NPRC A-55C CC combined with ET-2 A985G GG increased to 4.673 and to 5.529 when the MT genotype of AGT M235T, the EE genotype of eNOS Glu298Asp, the GG genotype of ET-2 A985G, and the CC genotype of NPRC A-55C were combined. MDR showed that ET-2/NPRC is the best model (OR = 4.002; 95%CI = 2.1597-7.4159). The CC genotype for NPRC A-55C and the G allele for ET-2 A985G were associated with susceptibility to EH. Although the contributions of the candidate genes differ, they may have cooperative effects on conferring risk for EH. Moreover, potential gene-gene interactions were found between ET-2 A985G and NPRC A-55C in EH.
authors.affiliation: Department of Clinical Laboratory, The First People's Hospital of Kunming, The Key Clinical Molecular Biology Laboratory of Kunming, Kunming, China dachunhu@163.com.
authors.forename: D C
authors.initials: DC
authors.lastname: Hu
authors.affiliation: Department of Clinical Laboratory, The First People's Hospital of Kunming, The Key Clinical Molecular Biology Laboratory of Kunming, Kunming, China.
authors.forename: X L
authors.initials: XL
authors.lastname: Zhao
authors.affiliation: Department of Clinical Laboratory, The First People's Hospital of Kunming, The Key Clinical Molecular Biology Laboratory of Kunming, Kunming, China.
authors.forename: J C
authors.initials: JC
authors.lastname: Shao
authors.affiliation: Department of Clinical Laboratory, The First People's Hospital of Kunming, The Key Clinical Molecular Biology Laboratory of Kunming, Kunming, China.
authors.forename: W
authors.initials: W
authors.lastname: Wang
authors.affiliation: Department of Clinical Laboratory, The First People's Hospital of Kunming, The Key Clinical Molecular Biology Laboratory of Kunming, Kunming, China.
authors.forename: J
authors.initials: J
authors.lastname: Qian
authors.affiliation: Department of Clinical Laboratory, The First People's Hospital of Kunming, The Key Clinical Molecular Biology Laboratory of Kunming, Kunming, China.
authors.forename: A H
authors.initials: AH
authors.lastname: Chen
authors.affiliation: Department of Clinical Laboratory, The First People's Hospital of Kunming, The Key Clinical Molecular Biology Laboratory of Kunming, Kunming, China.
authors.forename: H Q
authors.initials: HQ
authors.lastname: Zhang
authors.affiliation: Department of Clinical Laboratory, The First People's Hospital of Kunming, The Key Clinical Molecular Biology Laboratory of Kunming, Kunming, China.
authors.forename: H
authors.initials: H
authors.lastname: Guo
authors.affiliation: Department of Clinical Laboratory, The First People's Hospital of Kunming, The Key Clinical Molecular Biology Laboratory of Kunming, Kunming, China.
authors.forename: J
authors.initials: J
authors.lastname: Jiang
authors.affiliation: Department of Clinical Laboratory, The First People's Hospital of Kunming, The Key Clinical Molecular Biology Laboratory of Kunming, Kunming, China.
authors.forename: H Y
authors.initials: HY
authors.lastname: Li
mesh_terms: D000368:Aged; D000483:Alleles; D016022:Case-Control Studies; D004843:Epistasis, Genetic; D000075222:Essential Hypertension; D005260:Female; D005787:Gene Frequency; D056726:Genetic Association Studies; D056426:Genetic Loci; D005838:Genotype; D006801:Humans; D006973:Hypertension / Q000235:genetics* / Q000503:physiopathology; D008297:Male; D008875:Middle Aged; D016017:Odds Ratio; D011110:Polymorphism, Genetic