title: Controlled drug release from antibiotic-loaded layered double hydroxide coatings on porous titanium implants in a mouse model.
abstract: As an alternative to degradable organic coatings the possibility of using layered double hydroxides (LDHs) to generate implant coatings for controlled drug delivery was evaluated in vivo and in vitro. Coatings prepared from LDH suspensions dissolved slowly and appeared compatible with cultured cells. LDH coatings loaded with an antibiotic resulted in antibacterial effects in vitro. The LDH coating prolonged the drug release period and improved the proliferation of adherent cells in comparison to pure drug coatings. However, during incubation in physiological solutions the LDH coatings became brittle and pieces occasionally detached from the surface. For stress protection porous titanium implants were investigated as a substrate for the coatings. The pores prevented premature detachment of the coatings. To evaluate the coated porous implants in vivo a mouse model was established. To monitor bacterial infection of implants noninvasive in vivo imaging was used to monitor luminescently labeled Pseudomonas aeruginosa. In this model porous implants with antibiotic-loaded LDH coatings could antagonize bacterial infections for over 1 week. The findings provide evidence that delayed drug delivery from LDH coatings could be feasible in combination with structured implant surfaces.
authors.affiliation: Helmholtz Centre for Infection Research, Inhoffenstrasse 7, 38124, Braunschweig, Germany; Gomal Center of Biochemistry and Biotechnology (GCBB), Gomal University, Dera Ismail Khan, Pakistan.
authors.forename: Muhammad
authors.initials: M
authors.lastname: Badar
authors.forename: Muhammad Imran
authors.initials: MI
authors.lastname: Rahim
authors.forename: Marc
authors.initials: M
authors.lastname: Kieke
authors.forename: Thomas
authors.initials: T
authors.lastname: Ebel
authors.forename: Manfred
authors.initials: M
authors.lastname: Rohde
authors.forename: Hansjörg
authors.initials: H
authors.lastname: Hauser
authors.forename: Peter
authors.initials: P
authors.lastname: Behrens
authors.forename: Peter P
authors.initials: PP
authors.lastname: Mueller
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002939:Ciprofloxacin / Q000494:pharmacology; D020099:Coated Materials, Biocompatible / Q000494:pharmacology*; D003692:Delayed-Action Preparations / Q000494:pharmacology; D005260:Female; D006878:Hydroxides / Q000494:pharmacology*; D051379:Mice; D008807:Mice, Inbred BALB C; D023421:Models, Animal; D041681:NIH 3T3 Cells; D016062:Porosity; D014025:Titanium / Q000494:pharmacology*; D014835:Volatilization
chemical_list: D000900:Anti-Bacterial Agents; D020099:Coated Materials, Biocompatible; D003692:Delayed-Action Preparations; D006878:Hydroxides; D002939:Ciprofloxacin; C031356:hydroxide ion; D014025:Titanium
keywords: bacterial biofilm; degradable implant coating; implant infection; layered double hydroxides; local drug delivery