title: Lipid-soluble ginseng extract inhibits invasion and metastasis of B16F10 melanoma cells.
abstract: This study was performed to elucidate the effect of a lipid-soluble ginseng extract (LSGE) on cancer invasion and metastasis. The LSGE, even at noncytotoxic concentrations, potently inhibited invasion and migration of B16F10 mouse melanoma cells in a dose-dependent manner. In the presence of 3 μg/mL of LSGE, the invasion and migration of B16F10 cells were significantly inhibited by 98.1% and 71.4%, respectively. Furthermore, the LSGE decreased mRNA and protein levels of matrix metalloproteinase (MMP)-2 in B16F10 cells, leading to a decrease in MMP-2 activity. After B16F10 cells were intravenously injected in the tail vein of C57BL/6 mice, 1000 mg/kg/day of LSGE was orally administered for 13 days, after which lung metastasis of cancer cells was inhibited by 59.3%. These findings indicate that LSGE inhibits cancer cell invasion and migration in vitro and lung metastasis of melanoma cells in vivo by inhibiting MMP-2 expression.
authors.affiliation: 1 Bio-Evaluation Center, Korea Research Institute of Bioscience and Biotechnology , Ochang, Republic of Korea.
authors.forename: Jieun
authors.initials: J
authors.lastname: Yun
authors.forename: Bo Geun
authors.initials: BG
authors.lastname: Kim
authors.forename: Jong Soon
authors.initials: JS
authors.lastname: Kang
authors.forename: Song-Kyu
authors.initials: SK
authors.lastname: Park
authors.forename: Kiho
authors.initials: K
authors.lastname: Lee
authors.forename: Dong-Hoon
authors.initials: DH
authors.lastname: Hyun
authors.forename: Hwan Mook
authors.initials: HM
authors.lastname: Kim
authors.forename: Man-Jin
authors.initials: MJ
authors.lastname: In
authors.forename: Dong Chung
authors.initials: DC
authors.lastname: Kim
mesh_terms: D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology / Q000627:therapeutic use*; D045744:Cell Line, Tumor; D004305:Dose-Response Relationship, Drug; D008175:Lung Neoplasms / Q000378:metabolism / Q000517:prevention & control* / Q000556:secondary; D020778:Matrix Metalloproteinase 2 / Q000235:genetics / Q000378:metabolism*; D008545:Melanoma / Q000188:drug therapy* / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D008810:Mice, Inbred C57BL; D009361:Neoplasm Invasiveness; D005894:Panax; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use*; D012333:RNA, Messenger / Q000378:metabolism; D012995:Solubility
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D010936:Plant Extracts; D012333:RNA, Messenger; D020778:Matrix Metalloproteinase 2
keywords: cancer invasion; lipid-soluble ginseng extract; lung metastasis; matrix metalloprotease-2