title: Synergistic anticancer activity of photo- and chemoresponsive nanoformulation based on polylysine-functionalized graphene.
abstract: Multimodal therapeutic agents based on nanomaterials for cancer combination therapy have attracted increasing attention. In this report, a novel photo- and chemoactive nanohybrid was fabricated by assembling photosensitizer Zn(II)-phthalocyanine (ZnPc) and anticancer drug doxorubicin (DOX) on the biocompatible poly-l-lysine (PLL)-grafted graphene (G-PLL). This nanocomplex of G-PLL/DOX/ZnPc showed excellent physiochemical properties, including high solubility and stability in biological solutions, high drug loading efficiency, pH-triggered drug release, and ability to generalize (1)O2 under light excitation. Compared to free drug molecules, cells treated with G-PLL/DOX/ZnPc showed a higher cellular uptake. In particular, G-PLL/DOX/ZnPc elicited a remarkable synergistic anticancer activity owing to combined photodynamic and chemotherapeutic effects. The combination dose reduction indexes revealed that combining DOX with ZnPc provided strong synergistic effects (combination index < 0.1) against three cancer cell lines tested (HeLa, MCF-7, and B16). Thus, this study demonstrates programmable dual-modality therapy exemplified by G-PLL/DOX/ZnPc to synergistically treat cancers.
authors.affiliation: Department of Biophysics, School of Life Science and Technology, University of Electronic Science and Technology of China , Chengdu, Sichuan 610054, P. R. China.
authors.forename: Chunhui
authors.initials: C
authors.lastname: Wu
authors.forename: Qiuming
authors.initials: Q
authors.lastname: He
authors.forename: Anni
authors.initials: A
authors.lastname: Zhu
authors.forename: Dan
authors.initials: D
authors.lastname: Li
authors.forename: Min
authors.initials: M
authors.lastname: Xu
authors.forename: Hong
authors.initials: H
authors.lastname: Yang
authors.forename: Yiyao
authors.initials: Y
authors.lastname: Liu
mesh_terms: D000970:Antineoplastic Agents / Q000008:administration & dosage / Q000737:chemistry; D004317:Doxorubicin / Q000008:administration & dosage / Q000737:chemistry*; D016503:Drug Delivery Systems; D065546:Drug Liberation; D006367:HeLa Cells; D006801:Humans; D053758:Nanoparticles / Q000008:administration & dosage / Q000737:chemistry*; D009369:Neoplasms / Q000188:drug therapy*; D017319:Photosensitizing Agents / Q000008:administration & dosage / Q000737:chemistry; D011092:Polyethylene Glycols / Q000008:administration & dosage / Q000737:chemistry; D011107:Polylysine / Q000008:administration & dosage / Q000737:chemistry
chemical_list: D000970:Antineoplastic Agents; D017319:Photosensitizing Agents; D011107:Polylysine; D011092:Polyethylene Glycols; D004317:Doxorubicin
keywords: chemotherapy; photodynamic therapy; photosensitizer; poly-l-lysine-functionalized graphene; synergistic anticancer efficiency