title: Hsp10 nuclear localization and changes in lung cells response to cigarette smoke suggest novel roles for this chaperonin.
abstract: Heat-shock protein (Hsp)10 is the co-chaperone for Hsp60 inside mitochondria, but it also resides outside the organelle. Variations in its levels and intracellular distribution have been documented in pathological conditions, e.g. cancer and chronic obstructive pulmonary disease (COPD). Here, we show that Hsp10 in COPD undergoes changes at the molecular and subcellular levels in bronchial cells from human specimens and derived cell lines, intact or subjected to stress induced by cigarette smoke extract (CSE). Noteworthy findings are: (i) Hsp10 occurred in nuclei of epithelial and lamina propria cells of bronchial mucosa from non-smokers and smokers; (ii) human bronchial epithelial (16HBE) and lung fibroblast (HFL-1) cells, in vitro, showed Hsp10 in the nucleus, before and after CSE exposure; (iii) CSE stimulation did not increase the levels of Hsp10 but did elicit qualitative changes as indicated by molecular weight and isoelectric point shifts; and (iv) Hsp10 nuclear levels increased after CSE stimulation in HFL-1, indicating cytosol to nucleus migration, and although Hsp10 did not bind DNA, it bound a DNA-associated protein.
authors.affiliation: Istituto Euro-Mediterraneo di Scienza e Tecnologia (IEMEST), Palermo, Italy Dipartimento di Biomedicina Sperimentale e Neuroscienze Cliniche, Università degli Studi di Palermo, Palermo, Italy.
authors.forename: Simona
authors.initials: S
authors.lastname: Corrao
authors.affiliation: Dipartimento di Biomedicina Sperimentale e Neuroscienze Cliniche, Università degli Studi di Palermo, Palermo, Italy.
authors.forename: Rita
authors.initials: R
authors.lastname: Anzalone
authors.affiliation: Istituto Euro-Mediterraneo di Scienza e Tecnologia (IEMEST), Palermo, Italy Dipartimento di Biomedicina Sperimentale e Neuroscienze Cliniche, Università degli Studi di Palermo, Palermo, Italy.
authors.forename: Melania
authors.initials: M
authors.lastname: Lo Iacono
authors.affiliation: Istituto Euro-Mediterraneo di Scienza e Tecnologia (IEMEST), Palermo, Italy Dipartimento di Biomedicina Sperimentale e Neuroscienze Cliniche, Università degli Studi di Palermo, Palermo, Italy.
authors.forename: Tiziana
authors.initials: T
authors.lastname: Corsello
authors.affiliation: Laboratorio di Citoimmunopatologia dell'apparato cardio-respiratorio, Fondazione 'S. Maugeri' IRCCS, Istituto di Veruno, Veruno (NO), Italy.
authors.forename: Antonino
authors.initials: A
authors.lastname: Di Stefano
authors.affiliation: HSR Giglio, Cefalù (PA), Italy.
authors.forename: Silvestro Ennio
authors.initials: SE
authors.lastname: D'Anna
authors.affiliation: Divisione di Pneumologia, Fondazione 'S. Maugeri' IRCCS, Istituto di Veruno, Veruno (NO), Italy.
authors.forename: Bruno
authors.initials: B
authors.lastname: Balbi
authors.affiliation: Fondazione 'S. Maugeri' IRCCS, Istituto Scientifico di Cassano delle Murge, Cassano delle Murge (BA), Italy.
authors.forename: Mauro
authors.initials: M
authors.lastname: Carone
authors.affiliation: Dipartimento STEBICEF, Istituto Telethon Dulbecco c/o Universita' degli Studi di Palermo, Sezione di Biologia Cellulare, Palermo, Italy.
authors.forename: Anna
authors.initials: A
authors.lastname: Sala
authors.affiliation: Dipartimento STEBICEF, Istituto Telethon Dulbecco c/o Universita' degli Studi di Palermo, Sezione di Biologia Cellulare, Palermo, Italy.
authors.forename: Davide
authors.initials: D
authors.lastname: Corona
authors.affiliation: Department of Biology and Ecology "La Tuscia" University, Viterbo, Italy.
authors.forename: Anna Maria
authors.initials: AM
authors.lastname: Timperio
authors.affiliation: Department of Biology and Ecology "La Tuscia" University, Viterbo, Italy.
authors.forename: Lello
authors.initials: L
authors.lastname: Zolla
authors.affiliation: Dipartimento di Biomedicina Sperimentale e Neuroscienze Cliniche, Università degli Studi di Palermo, Palermo, Italy.
authors.forename: Felicia
authors.initials: F
authors.lastname: Farina
authors.affiliation: Department of Microbiology and Immunology, School of Medicine, University of Maryland at Baltimore, Baltimore, MD, USA IMET, Columbus Center, Baltimore, MD, USA.
authors.forename: Everly Conway
authors.initials: EC
authors.lastname: de Macario
authors.affiliation: Istituto Euro-Mediterraneo di Scienza e Tecnologia (IEMEST), Palermo, Italy Department of Microbiology and Immunology, School of Medicine, University of Maryland at Baltimore, Baltimore, MD, USA IMET, Columbus Center, Baltimore, MD, USA.
authors.forename: Alberto J L
authors.initials: AJ
authors.lastname: Macario
authors.affiliation: Istituto Euro-Mediterraneo di Scienza e Tecnologia (IEMEST), Palermo, Italy Dipartimento di Biomedicina Sperimentale e Neuroscienze Cliniche, Università degli Studi di Palermo, Palermo, Italy.
authors.forename: Francesco
authors.initials: F
authors.lastname: Cappello
authors.affiliation: Istituto Euro-Mediterraneo di Scienza e Tecnologia (IEMEST), Palermo, Italy Dipartimento di Biomedicina Sperimentale e Neuroscienze Cliniche, Università degli Studi di Palermo, Palermo, Italy giampylr@hotmail.com giampiero.larocca@unipa.it.
authors.forename: Giampiero
authors.identifier: http://orcid.org/0000-0002-1334-5054
authors.initials: G
authors.lastname: La Rocca
mesh_terms: D000368:Aged; D001980:Bronchi / Q000378:metabolism; D002467:Cell Nucleus / Q000378:metabolism; D018835:Chaperonin 10 / Q000378:metabolism*; D018834:Chaperonin 60 / Q000378:metabolism; D003198:Computer Simulation; D003600:Cytosol / Q000378:metabolism; D004247:DNA / Q000737:chemistry; D004847:Epithelial Cells / Q000166:cytology* / Q000378:metabolism; D005260:Female; D005347:Fibroblasts / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D007526:Isoelectric Point; D008168:Lung / Q000166:cytology*; D008297:Male; D008875:Middle Aged; D024101:Mitochondrial Proteins / Q000378:metabolism; D008970:Molecular Weight; D009707:Nucleosomes / Q000737:chemistry; D029424:Pulmonary Disease, Chronic Obstructive / Q000378:metabolism; D012129:Respiratory Function Tests; D012906:Smoke; D012907:Smoking; D062789:Tobacco Products
chemical_list: D018835:Chaperonin 10; D018834:Chaperonin 60; C060293:HSPD1 protein, human; D024101:Mitochondrial Proteins; D009707:Nucleosomes; D012906:Smoke; D004247:DNA
keywords: COPD; Hsp10; bronchial epithelial cells; lung fibroblasts; nuclear localization