title: Adhesion properties of Lactobacillus rhamnosus mucus-binding factor to mucin and extracellular matrix proteins.
abstract: We previously described potential probiotic Lactobacillus rhamnosus strains, isolated from fermented mare milk produced in Sumbawa Island, Indonesia, which showed high adhesion to porcine colonic mucin (PCM) and extracellular matrix (ECM) proteins. Recently, mucus-binding factor (MBF) was found in the GG strain of L. rhamnosus as a mucin-binding protein. In this study, we assessed the ability of recombinant MBF protein from the FSMM22 strain, one of the isolates of L. rhamnosus from fermented Sumbawa mare milk, to adhere to PCM and ECM proteins by overlay dot blot and Biacore assays. MBF bound to PCM, laminin, collagen IV, and fibronectin with submicromolar dissociation constants. Adhesion of the FSMM22 mbf mutant strain to PCM and ECM proteins was significantly less than that of the wild-type strain. Collectively, these results suggested that MBF contribute to L. rhamnosus host colonization via mucin and ECM protein binding.
authors.affiliation: a Department of Animal Science, School of Veterinary Medicine , Kitasato University , Towada, Japan.
authors.forename: Keita
authors.initials: K
authors.lastname: Nishiyama
authors.forename: Koichi
authors.initials: K
authors.lastname: Nakamata
authors.forename: Shintaro
authors.initials: S
authors.lastname: Ueno
authors.forename: Akari
authors.initials: A
authors.lastname: Terao
authors.forename: Ni Putu Desy
authors.initials: NP
authors.lastname: Aryantini
authors.forename: I Nengah
authors.initials: IN
authors.lastname: Sujaya
authors.forename: Kenji
authors.initials: K
authors.lastname: Fukuda
authors.forename: Tadasu
authors.initials: T
authors.lastname: Urashima
authors.forename: Yuji
authors.initials: Y
authors.lastname: Yamamoto
authors.forename: Takao
authors.initials: T
authors.lastname: Mukai
mesh_terms: D001422:Bacterial Adhesion; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D002473:Cell Wall / Q000378:metabolism; D003001:Cloning, Molecular; D016326:Extracellular Matrix Proteins / Q000378:metabolism*; D052201:Lacticaseibacillus rhamnosus / Q000166:cytology / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D009077:Mucins / Q000378:metabolism*; D009154:Mutation; D017421:Sequence Analysis
chemical_list: D001426:Bacterial Proteins; D016326:Extracellular Matrix Proteins; D009077:Mucins
keywords: Lactobacillus rhamnosus; bacterial adhesion; extracellular matrix (ECM) proteins; mucus-binding factor