title: Development of a GnRH-PGF2α-progesterone-based synchronization protocol with eCG for inducing single and double ovulations in beef cattle.
abstract: Experiments were designed to investigate the effect of different doses and timing of an eCG treatment given during GnRH-based synchronization protocols on follicular dynamics and fertility in cattle. In Exp. 1, Angus heifers (n = 50) received a 7-d Ovsynch + progesterone protocol (on d 0, GnRH and progesterone insert were administered; on d 7, progesterone insert was removed and PGF2α was injected; and on d 9.5, GnRH was injected 56 h after progesterone removal) with eCG (0, 300, 500, 700, or 1,000 IU) administered on d 7. In Exp. 2, Angus cows (n = 27) received the same protocol as Exp. 1 and were assigned randomly to receive 0 or 400 IU eCG i.m. on d 2 or 7. In Exp. 3, Angus cows (n = 18) received a 6-d Ovsynch + progesterone protocol and were randomly assigned to receive 0 or 800 IU eCG on d 3 of the protocol (Exp. 3a). A pilot field trial was also performed using the same treatments in suckled Angus-cross cows (n = 72; Exp. 3b). In Exp. 4, beef heifers (n = 200) were assigned randomly to the same treatments as in Exp. 3, but the second GnRH was not given, with Holstein bulls introduced on d 6. In Exp. 5, Angus cows (n = 12) received the same treatment as in Exp. 3, but were not inseminated. Progesterone concentrations were assessed in plasma collected during the estrous cycle following synchronization. Ultrasonography was used to monitor ovarian dynamics and to diagnose pregnancy. In Exp. 1, the mean number of ovulations was affected (P < 0.02) by the dose of eCG and the stage of follicular development when administered. Treatment with eCG on d 2 tended (P < 0.08) to extend the interval from PGF2α to ovulation, but was not successful in inducing double ovulations. In contrast, eCG on d 3 increased (P < 0.01) the number of cows with double ovulation when administered i.m. and increased (P < 0.04) pregnancy rate in single ovulating heifers after bull breeding (68.0 vs. 53.1%). This treatment also elevated progesterone concentrations during the estrous cycle following synchronization. Thus, the mechanism by which administration of eCG on d 3 of the synchronization increased pregnancy rates may be through supporting development of a healthy follicle and subsequent corpus luteum capable of secreting increased concentrations of progesterone during early pregnancy. In conclusion, strategic administration of eCG during a synchronization protocol can be used to improve reproductive performance through increased pregnancy rates in single ovulating animals as well as the induction of twin ovulations for twinning.
authors.affiliation: AgResearch Invermay Agricultural Centre, Dunedin 9013, New Zealand marcelo.martinez@agresearch.co.nz.
authors.forename: M F
authors.initials: MF
authors.lastname: Martinez
authors.affiliation: AgResearch Invermay Agricultural Centre, Dunedin 9013, New Zealand.
authors.forename: D
authors.initials: D
authors.lastname: Tutt
authors.affiliation: AgResearch Invermay Agricultural Centre, Dunedin 9013, New Zealand.
authors.forename: L D
authors.initials: LD
authors.lastname: Quirke
authors.affiliation: Integrated Foods Ltd., 266 Childers Road, Gisborne 4010, New Zealand.
authors.forename: G
authors.initials: G
authors.lastname: Tattersfield
authors.affiliation: AgResearch Invermay Agricultural Centre, Dunedin 9013, New Zealand.
authors.forename: J L
authors.initials: JL
authors.lastname: Juengel
mesh_terms: D000818:Animals; D001947:Breeding / Q000379:methods; D002417:Cattle / Q000502:physiology*; D006063:Chorionic Gonadotropin / Q000494:pharmacology*; D015237:Dinoprost / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D030762:Estrous Cycle / Q000187:drug effects; D004973:Estrus Synchronization / Q000379:methods; D005260:Female; D005298:Fertility / Q000187:drug effects; D007987:Gonadotropin-Releasing Hormone / Q000494:pharmacology*; D006064:Gonadotropins, Equine / Q000494:pharmacology; D008297:Male; D006080:Ovarian Follicle / Q000187:drug effects; D010060:Ovulation / Q000187:drug effects* / Q000502:physiology; D010062:Ovulation Induction / Q000379:methods / Q000662:veterinary*; D011247:Pregnancy; D018873:Pregnancy Rate; D011374:Progesterone / Q000494:pharmacology*
chemical_list: D006063:Chorionic Gonadotropin; D006064:Gonadotropins, Equine; D007987:Gonadotropin-Releasing Hormone; D011374:Progesterone; D015237:Dinoprost
keywords: beef cattle; double ovulation; equine chorionic gonadotropin; estrus synchronization; ovulation synchronization; twinning