title: Quantitative proteomics of the human skin secretome reveal a reduction in immune defense mediators in ectodermal dysplasia patients.
abstract: In healthy human skin host defense molecules such as antimicrobial peptides (AMPs) contribute to skin immune homeostasis. In patients with the congenital disease ectodermal dysplasia (ED) skin integrity is disturbed and as a result patients have recurrent skin infections. The disease is characterized by developmental abnormalities of ectodermal derivatives and absent or reduced sweating. We hypothesized that ED patients have a reduced skin immune defense because of the reduced ability to sweat. Therefore, we performed a label-free quantitative proteome analysis of wash solution of human skin from ED patients or healthy individuals. A clear-cut difference between both cohorts could be observed in cellular processes related to immunity and host defense. In line with the extensive underrepresentation of proteins of the immune system, dermcidin, a sweat-derived AMP, was reduced in its abundance in the skin secretome of ED patients. In contrast, proteins involved in metabolic/catabolic and biosynthetic processes were enriched in the skin secretome of ED patients. In summary, our proteome profiling provides insights into the actual situation of healthy versus diseased skin. The systematic reduction in immune system and defense-related proteins may contribute to the high susceptibility of ED patients to skin infections and altered skin colonization.
authors.affiliation: Department of Dermatology, Eberhard-Karls-University Tübingen, Tübingen, Germany. Electronic address: marc.burian@med.uni-tuebingen.de.
authors.forename: Marc
authors.initials: M
authors.lastname: Burian
authors.affiliation: Proteome Center Tübingen, Eberhard-Karls-University Tübingen, Tübingen, Germany.
authors.forename: Ana
authors.initials: A
authors.lastname: Velic
authors.affiliation: Proteome Center Tübingen, Eberhard-Karls-University Tübingen, Tübingen, Germany.
authors.forename: Katarina
authors.initials: K
authors.lastname: Matic
authors.affiliation: Department of Dermatology, Eberhard-Karls-University Tübingen, Tübingen, Germany.
authors.forename: Stephanie
authors.initials: S
authors.lastname: Günther
authors.affiliation: Department of Dermatology, Eberhard-Karls-University Tübingen, Tübingen, Germany.
authors.forename: Beatrice
authors.initials: B
authors.lastname: Kraft
authors.affiliation: Department of Dermatology, Eberhard-Karls-University Tübingen, Tübingen, Germany.
authors.forename: Lena
authors.initials: L
authors.lastname: Gonser
authors.affiliation: Department of Dermatology, Eberhard-Karls-University Tübingen, Tübingen, Germany.
authors.forename: Stephan
authors.initials: S
authors.lastname: Forchhammer
authors.affiliation: B.R.A.I.N AG, Biotechnology Research And Information Network, Zwingenberg, Germany.
authors.forename: Yvonne
authors.initials: Y
authors.lastname: Tiffert
authors.affiliation: B.R.A.I.N AG, Biotechnology Research And Information Network, Zwingenberg, Germany.
authors.forename: Christian
authors.initials: C
authors.lastname: Naumer
authors.affiliation: B.R.A.I.N AG, Biotechnology Research And Information Network, Zwingenberg, Germany.
authors.forename: Michael
authors.initials: M
authors.lastname: Krohn
authors.affiliation: Department of Dermatology, University of Regensburg, Regensburg, Germany.
authors.forename: Mark
authors.initials: M
authors.lastname: Berneburg
authors.affiliation: Department of Dermatology, Eberhard-Karls-University Tübingen, Tübingen, Germany.
authors.forename: Amir S
authors.initials: AS
authors.lastname: Yazdi
authors.affiliation: Proteome Center Tübingen, Eberhard-Karls-University Tübingen, Tübingen, Germany.
authors.forename: Boris
authors.initials: B
authors.lastname: Maček
authors.affiliation: Department of Dermatology, Eberhard-Karls-University Tübingen, Tübingen, Germany. Electronic address: birgit.schittek@med.uni-tuebingen.de.
authors.forename: Birgit
authors.initials: B
authors.lastname: Schittek
mesh_terms: D000328:Adult; D000818:Animals; D016022:Case-Control Studies; D004195:Disease Models, Animal; D004476:Ectodermal Dysplasia / Q000276:immunology* / Q000378:metabolism*; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009824:Ointments; D010455:Peptides / Q000008:administration & dosage / Q000378:metabolism* / Q000627:therapeutic use; D040901:Proteomics; D012867:Skin / Q000378:metabolism*; D013207:Staphylococcal Skin Infections / Q000188:drug therapy / Q000382:microbiology; D013211:Staphylococcus aureus; D013545:Sweat Glands / Q000378:metabolism
chemical_list: D009824:Ointments; D010455:Peptides; C442243:dermcidin