title: Biochemical characteristics, adhesion, and cytotoxicity of environmental and clinical isolates of Herbaspirillum spp.
abstract: Herbaspirillum bacteria are best known as plant growth-promoting rhizobacteria but have also been recovered from clinical samples. Here, biochemical tests, matrix-assisted laser deionization-time of flight (MALDI-TOF) mass spectrometry, adherence, and cytotoxicity to eukaryotic cells were used to compare clinical and environmental isolates of Herbaspirillum spp. Discrete biochemical differences were observed between human and environmental strains. All strains adhered to HeLa cells at low densities, and cytotoxic effects were discrete, supporting the view that Herbaspirillum bacteria are opportunists with low virulence potential.
authors.affiliation: Departamento de Análises Clínicas, Universidade Federal do Paraná, Setor de Ciências da Saúde, Curitiba, Paraná, Brazil.
authors.forename: Ana C Q
authors.initials: AC
authors.lastname: Marques
authors.affiliation: Departamento de Biologia Estrutural Molecular e Genética, Universidade Estadual de Ponta Grossa, Ponta Grossa, Paraná, Brazil.
authors.forename: Katia S
authors.initials: KS
authors.lastname: Paludo
authors.affiliation: Departamento de Análises Clínicas, Universidade Federal do Paraná, Setor de Ciências da Saúde, Curitiba, Paraná, Brazil.
authors.forename: Cibelle B
authors.initials: CB
authors.lastname: Dallagassa
authors.affiliation: Departamento de Análises Clínicas, Universidade Federal do Paraná, Setor de Ciências da Saúde, Curitiba, Paraná, Brazil.
authors.forename: Monica
authors.initials: M
authors.lastname: Surek
authors.affiliation: Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, Setor de Ciências Biológicas, Curitiba, Paraná, Brazil.
authors.forename: Fábio O
authors.initials: FO
authors.lastname: Pedrosa
authors.affiliation: Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, Setor de Ciências Biológicas, Curitiba, Paraná, Brazil.
authors.forename: Emanuel M
authors.initials: EM
authors.lastname: Souza
authors.affiliation: Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, Setor de Ciências Biológicas, Curitiba, Paraná, Brazil.
authors.forename: Leonardo M
authors.initials: LM
authors.lastname: Cruz
authors.affiliation: Department of Pediatrics, University of Michigan, Ann Arbor, Michigan, USA.
authors.forename: John J
authors.initials: JJ
authors.lastname: LiPuma
authors.affiliation: Departamento de Patologia Básica, Universidade Federal do Paraná, Setor de Ciências Biológicas, Curitiba, Paraná, Brazil.
authors.forename: Sílvio M
authors.initials: SM
authors.lastname: Zanata
authors.affiliation: Departamento de Análises Clínicas, Universidade Federal do Paraná, Setor de Ciências da Saúde, Curitiba, Paraná, Brazil.
authors.forename: Fabiane G M
authors.initials: FG
authors.lastname: Rego
authors.affiliation: Departamento de Análises Clínicas, Universidade Federal do Paraná, Setor de Ciências da Saúde, Curitiba, Paraná, Brazil fpicheth@ufpr.br.
authors.forename: Cyntia M T
authors.initials: CM
authors.lastname: Fadel-Picheth
mesh_terms: D001422:Bacterial Adhesion / Q000502:physiology*; D002470:Cell Survival; D004783:Environmental Microbiology; D016905:Gram-Negative Bacterial Infections / Q000382:microbiology*; D006367:HeLa Cells; D042803:Herbaspirillum / Q000737:chemistry / Q000145:classification / Q000472:pathogenicity* / Q000502:physiology*; D006801:Humans; D010802:Phylogeny; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization