title: Autocrine stimulation of IL-10 is critical to the enrichment of IL-10-producing CD40(hi)CD5(+) regulatory B cells in vitro and in vivo.
abstract: IL-10-producing B (Breg) cells regulate various immune responses. However, their phenotype remains unclear. CD40 expression was significantly increased in B cells by LPS, and the Breg cells were also enriched in CD40(hi)CD5(+) B cells. Furthermore, CD40 expression on Breg cells was increased by IL-10, CD40 ligand, and B cell-activating factor, suggesting that CD40(hi) is a common phenotype of Breg cells. LPS-induced CD40 expression was largely suppressed by an anti-IL-10 receptor antibody and in IL-10(-/-)CD5(+)CD19(+) B cells. The autocrine effect of IL-10 on the CD40 expression was largely suppressed by an inhibitor of JAK/STAT3. In vivo, the LPS treatment increased the population of CD40(hi)CD5(+) Breg cells in mice. However, the population of CD40(hi)CD5(+) B cells was minimal in IL-10(-/-) mice by LPS. Altogether, our findings show that Breg cells are largely enriched in CD40(hi)CD5(+) B cells and the autocrine effect of IL-10 is critical to the formation of CD40(hi)CD5(+) Breg cells.
authors.affiliation: School of Medicine, Konkuk University, Chungju 380-701, Korea.
authors.forename: Hyuk Soon
authors.initials: HS
authors.lastname: Kim
authors.affiliation: College of Medicine, Korea University, Seoul 136-701, Korea.
authors.forename: Jun Ho
authors.initials: JH
authors.lastname: Lee
authors.affiliation: School of Medicine, Konkuk University, Chungju 380-701, Korea.
authors.forename: Hee Dong
authors.initials: HD
authors.lastname: Han
authors.affiliation: School of Medicine, Konkuk University, Chungju 380-701, Korea.
authors.forename: A-Ram
authors.initials: AR
authors.lastname: Kim
authors.affiliation: School of Medicine, Konkuk University, Chungju 380-701, Korea.
authors.forename: Seung Taek
authors.initials: ST
authors.lastname: Nam
authors.affiliation: School of Medicine, Konkuk University, Chungju 380-701, Korea.
authors.forename: Hyun Woo
authors.initials: HW
authors.lastname: Kim
authors.affiliation: School of Medicine, Konkuk University, Chungju 380-701, Korea.
authors.forename: Young Hwan
authors.initials: YH
authors.lastname: Park
authors.affiliation: School of Medicine, Konkuk University, Chungju 380-701, Korea.
authors.forename: Dajeong
authors.initials: D
authors.lastname: Lee
authors.affiliation: School of Medicine, Konkuk University, Chungju 380-701, Korea.
authors.forename: Min Bum
authors.initials: MB
authors.lastname: Lee
authors.affiliation: School of Medicine, Konkuk University, Chungju 380-701, Korea.
authors.forename: Yeong Min
authors.initials: YM
authors.lastname: Park
authors.affiliation: College of Pharmacy, Sungkyunkwan University, Suwon 440-746, Korea.
authors.forename: Hyung Sik
authors.initials: HS
authors.lastname: Kim
authors.affiliation: College of Pharmacy, Duksung Women's University, Seoul 132-714, Korea.
authors.forename: Young Mi
authors.initials: YM
authors.lastname: Kim
authors.affiliation: National Research Laboratory of Molecular Virology, Department of Pathology, School of Medicine, The Catholic University of Korea, Seoul 137-701, Korea.
authors.forename: Ji Chang
authors.initials: JC
authors.lastname: You
authors.affiliation: School of Medicine, Konkuk University, Chungju 380-701, Korea.
authors.forename: Wahn Soo
authors.initials: WS
authors.lastname: Choi
mesh_terms: D000818:Animals; D019898:Autocrine Communication / Q000187:drug effects; D060151:B-Lymphocytes, Regulatory / Q000276:immunology / Q000378:metabolism*; D019013:CD40 Antigens / Q000378:metabolism*; D018956:CD5 Antigens / Q000378:metabolism*; D002454:Cell Differentiation / Q000187:drug effects; D016753:Interleukin-10 / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology; D053612:Janus Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism; D008070:Lipopolysaccharides / Q000633:toxicity; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics / Q000494:pharmacology; D050796:STAT3 Transcription Factor / Q000037:antagonists & inhibitors / Q000378:metabolism
chemical_list: D019013:CD40 Antigens; D018956:CD5 Antigens; D008070:Lipopolysaccharides; D011994:Recombinant Proteins; D050796:STAT3 Transcription Factor; D016753:Interleukin-10; D053612:Janus Kinases