title: Two-photon photolysis combined with a kilobeam array scanner to probe calcium signaling in cardiomyocytes.
abstract: Photorelease of caged compounds allows a fast and defined intracellular increase in calcium (Ca(2+)) or other biologically relevant substances or molecules without impinging on other cellular functions. In particular, two-photon photolysis (2PP) allows a spatially restricted uncaging in sub-femtoliter volumes. Here, we describe how to combine 2PP with a confocal kilobeam array scanner and provide an example where Ca(2+) is released in an isolated cardiac myocyte.
authors.affiliation: Institute for Molecular Cell Biology and Research Center for Molecular Imaging and Screening, School of Medicine, Saarland University, 66421 Homburg/Saar, Germany.
authors.forename: Benjamin
authors.initials: B
authors.lastname: Sauer
authors.affiliation: Institute for Molecular Cell Biology and Research Center for Molecular Imaging and Screening, School of Medicine, Saarland University, 66421 Homburg/Saar, Germany.
authors.forename: Martin
authors.initials: M
authors.lastname: Oberhofer
authors.affiliation: Institute for Molecular Cell Biology and Research Center for Molecular Imaging and Screening, School of Medicine, Saarland University, 66421 Homburg/Saar, Germany.
authors.forename: Peter
authors.initials: P
authors.lastname: Lipp
authors.affiliation: Institute for Molecular Cell Biology and Research Center for Molecular Imaging and Screening, School of Medicine, Saarland University, 66421 Homburg/Saar, Germany.
authors.forename: Lars
authors.initials: L
authors.lastname: Kaestner
mesh_terms: D020013:Calcium Signaling; D003584:Cytological Techniques / Q000379:methods*; D036641:Microscopy, Fluorescence, Multiphoton / Q000379:methods*; D032383:Myocytes, Cardiac / Q000502:physiology*; D061848:Optical Imaging / Q000379:methods*; D010782:Photolysis