title: Effects of Blocking αvβ₃ integrin by a recombinant RGD disintegrin on remodeling of wound healing after induction of incisional hernia in rats.
abstract: OBJECTIVE
  Incisional hernia (IH) is characterized by defective wound healing process. Disba-01, a αvb₃ integrin blocker has shown to control the rate of wound repair and therefore it could be a target for new wound healing therapies.The objective of the study was to determine the changes induced by Disba-01 on repair of wound healing after induced IH in rats.
  
  
  METHODS
  Thirty two male albino rats were submitted to IH and divided into 4 experimental groups: G1, placebo control; G2, DisBa-01-treated; G3, anti-αvβ₃ antibodies-treated and G4, anti-α₂ antibodies-treated. Histological. biochemical and extracellular matrix remodeling analysis of abdominal wall were evaluated.
  
  
  RESULTS
  After 14 days, 100% of the G2 did not present hernia, and the hernia ring was closed by a thin membrane. In contrast, all groups maintained incisional hernia. DisBa-01 also increased the number macrophages and fibroblasts and induced the formation of new vessels. Additionally, MMP-2 was strongly activated only in G2 (P<0.05). Anti- αvβ₃-integrin antibodies produced similar results than Disba-01 but not anti-α₂ integrin blocking antibodies.
  
  
  CONCLUSIONS
  These results strongly indicate that Disba-01 has an important role in the control of wound healing and the blocking of this integrin may be an interesting therapeutical strategy in IH.
authors.affiliation: Department of Medicine, São Carlos Federal University, São Carlos, SP, Brazil.
authors.forename: Claudio Ricardo de
authors.initials: CR
authors.lastname: Oliveira
authors.affiliation: Department of Physioterapy, Brasília Federal University, Brasília, DF, Brazil.
authors.forename: Rita de Cassia
authors.initials: Rde C
authors.lastname: Marqueti
authors.affiliation: Department of Gerontology, São Carlos Federal University, São Carlos, SP, Brazil.
authors.forename: Marcia Regina
authors.initials: MR
authors.lastname: Cominetti
authors.affiliation: São Carlos Federal University, São Carlos, SP, Brazil.
authors.forename: Estela Sant Ana
authors.initials: ES
authors.lastname: Vieira
authors.affiliation: Department of Physioterapy, São Carlos Federal University, São Carlos, SP, Brazil.
authors.forename: Juliana Uema
authors.initials: JU
authors.lastname: Ribeiro
authors.affiliation: Department of Physioterapy, São Carlos Federal University, São Carlos, SP, Brazil.
authors.forename: Carmen Lucia Salla
authors.initials: CL
authors.lastname: Pontes
authors.affiliation: Department of Medicine, São Carlos Federal University, São Carlos, SP, Brazil.
authors.forename: Audrey
authors.initials: A
authors.lastname: Borghi-Silva
authors.affiliation: São Carlos Federal University, São Carlos, SP, Brazil.
authors.forename: Heloisa Sobreiro
authors.initials: HS
authors.lastname: Selistre-de-Araujo
mesh_terms: D034861:Abdominal Wall; D000818:Animals; D003094:Collagen / Q000187:drug effects; D019483:Disintegrins / Q000494:pharmacology*; D005347:Fibroblasts / Q000187:drug effects; D006555:Hernia, Ventral / Q000188:drug therapy* / Q000378:metabolism; D039302:Integrin alphaVbeta3 / Q000037:antagonists & inhibitors* / Q000737:chemistry / Q000378:metabolism; D008264:Macrophages / Q000187:drug effects; D008297:Male; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism / Q000494:pharmacology*; D011183:Postoperative Complications / Q000517:prevention & control; D011897:Random Allocation; D017208:Rats, Wistar; D014945:Wound Healing / Q000187:drug effects* / Q000502:physiology
chemical_list: D019483:Disintegrins; D039302:Integrin alphaVbeta3; D003094:Collagen; D020778:Matrix Metalloproteinase 2