title: [Expression of cysteine-rich secretory protein 2 in patients with asthenozoospermia and its clinical significance].
abstract: OBJECTIVE
  To investigate the expression of cysteine-rich secretory protein 2 (CRISP2) in spermatozoa of patients with asthenozoospermia and explore its clinical significance.
  
  
  METHODS
  Semen samples were collected from 24 normal volunteers and 24 patients with asthenozoospermia for detecting CRISP2 mRNA and protein expressions using qRT-PCR and Western blotting, respectively. The correlation of CRISP2 expressions with sperm morphology, progressive motility and fertility prognosis were analyzed in patients with asthenozoospermia.
  
  
  RESULTS
  CRISP2 protein expression was obviously lowered in the ejaculated spermatozoa of patients with asthenozoospermia as compared to the normal volunteers, but no significant difference in CRISP2 mRNA expression was found between the two groups. Correlation analysis showed that CRISP2 protein expression was positively correlated with normal sperm morphology (r=0.6182, P=0.0037) and progressive motility (r=0.6309, P=0.0029). Follow-up study of the patients revealed a higher fertility rate in patients with a relatively high CRISP2 protein expression than in those with low CRISP2 protein expression (80.0% vs 20.0%, P=0.0230).
  
  
  CONCLUSIONS
  The expression level of CRISP2 protein is positively correlated with normal sperm morphology and progressive motility. A reduced CRISP2 protein expression indicates poor fertility prognosis of patients with asthenozoospermia, suggesting the potential value of CRISP2 as a novel therapeutic target for treating asthenozoospermia.
authors.affiliation: Department of Urology, Third Affiliated Hospital of Southern Medical University, Guangzhou 510630, China.E-mail: zhouatian@163.com.
authors.forename: Junhao
authors.initials: J
authors.lastname: Zhou
authors.forename: Kangyi
authors.initials: K
authors.lastname: Xue
authors.forename: Mingkun
authors.initials: M
authors.lastname: Chen
authors.forename: Qizhao
authors.initials: Q
authors.lastname: Zhou
authors.forename: Jiankun
authors.initials: J
authors.lastname: Yang
authors.forename: Jun
authors.initials: J
authors.lastname: Bian
authors.forename: Xin
authors.initials: X
authors.lastname: Li
authors.forename: Wenbin
authors.initials: W
authors.lastname: Guo
authors.forename: Hui
authors.initials: H
authors.lastname: Xia
authors.forename: Tao
authors.initials: T
authors.lastname: Qi
authors.forename: Cundong
authors.initials: C
authors.lastname: Liu
mesh_terms: D053627:Asthenozoospermia / Q000378:metabolism*; D016022:Case-Control Studies; D015815:Cell Adhesion Molecules; D005298:Fertility; D005500:Follow-Up Studies; D006023:Glycoproteins / Q000378:metabolism*; D006801:Humans; D008297:Male; D012333:RNA, Messenger; D013081:Sperm Motility; D013094:Spermatozoa / Q000378:metabolism
chemical_list: C062621:CRISP2 protein, human; D015815:Cell Adhesion Molecules; D006023:Glycoproteins; D012333:RNA, Messenger