title: Correlation of HER-2/Neu protein expression and HER2 gene amplification with clinical morphological parameters of nonsmall cell lung cancer.
abstract: A total of 218 operation specimens from patients with nonsmall cell lung cancer were examined. The expression of HER-2 protein was studied by the immunohistochemical method, amplification of HER2 gene and centromere part of chromosome 17 (CEP17) - by the in situ chromogenic hybridization method. Moderate correlation (r=0.38, p<0.001) between the immunohistochemical status and HER2 gene amplification was detected. A relationship between TNM clinical morphological parameters and HER-2 protein content and chromosome 17 polysomia (for N parameter), HER-2 protein content, and HER2 gene amplification (for tumor histogenesis) was detected. The content of HER-2 protein and amplification of HER2 and CEP17 correlated with the clinical morphological parameters of patients with nonsmall cell lung cancer.
authors.affiliation: Altai Affiliated Branch of N. N. Blokhin Russian Cancer Center, Russian Academy of Medical Sciences, Barnaul, Russia, pathol@soramn.ru.
authors.forename: D S
authors.initials: DS
authors.lastname: Kobyakov
authors.forename: A M
authors.initials: AM
authors.lastname: Avdalyan
authors.forename: I P
authors.initials: IP
authors.lastname: Bobrov
authors.forename: E Yu
authors.initials: EY
authors.lastname: Bychkova
authors.forename: A F
authors.initials: AF
authors.lastname: Lazarev
authors.forename: E L
authors.initials: EL
authors.lastname: Lushnikova
authors.forename: L M
authors.initials: LM
authors.lastname: Nepomnyashchikh
mesh_terms: D000328:Adult; D000368:Aged; D002289:Carcinoma, Non-Small-Cell Lung / Q000378:metabolism* / Q000473:pathology*; D002886:Chromosomes, Human, Pair 17 / Q000235:genetics*; D005260:Female; D005784:Gene Amplification / Q000235:genetics*; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008297:Male; D008875:Middle Aged; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000235:genetics* / Q000378:metabolism*
chemical_list: D018719:Erb-b2 Receptor Tyrosine Kinases; C508053:ERBB2 protein, human