title: [Extraction and purification technologies of total flavonoids from Aconitum tanguticum].
abstract: OBJECTIVE
  To optimize the extraction and purification technologies of total flavonoids from Aconitum tanguticum whole plant.
  
  
  METHODS
  With the content of total flavonoids as index, the optimum extraction conditions for the concentration, volume of alcohol, extracting time and times were selected by orthogonal optimized; Comparing the adsorption quantity (mg/g) and resolution (%), four kinds of macroporous adsorption resins including D101, AB-8, X-5 and XAD-16 were investigated for the enrichment ability of total flavonoids from Aconitum tanguticum; Concentration and pH value of sample, sampling amount, elution solvent and loading and elution velocity for the optimum adsorption resin were determined.
  
  
  RESULTS
  The content of total flavonoids in Aconitum tanguticum was about 4.39%; The optimum extraction technique was 70% alcohol reflux extraction for three times,each time for one hour, the ratio of material and liquid was 1:10 (w/v); The optimum purification technology was: using XAD-16 macroporous resin, the initial concentration of total flavonoids of Aconitum tanguticum was 8 mg/mL, the sampling amount was 112 mg/g dry resin, the pH value was 5, the loading velocity was 3 mL/min, the elution solvent was 70% ethanol and the elution velocity was 5 mL/min. Under the optimum conditions, the average content of total flavonoids was raised from 4.39% to 46.19%.
  
  
  CONCLUSIONS
  The optimum extraction and purification technologies for total flavonoids of Aconitum tanguticum were suitable for industrial production for its simplicity and responsibility.
authors.forename: Yan-Rong
authors.initials: YR
authors.lastname: Li
authors.forename: Li-Xin
authors.initials: LX
authors.lastname: Yan
authors.forename: Wei-Hong
authors.initials: WH
authors.lastname: Feng
authors.forename: Chun
authors.initials: C
authors.lastname: Li
authors.forename: Zhi-Min
authors.initials: ZM
authors.lastname: Wang
mesh_terms: D031841:Aconitum / Q000737:chemistry* / Q000254:growth & development; D000327:Adsorption; D002681:China; D000431:Ethanol / Q000737:chemistry; D005419:Flavonoids / Q000737:chemistry / Q000302:isolation & purification*; D006863:Hydrogen-Ion Concentration; D010946:Plants, Medicinal / Q000737:chemistry*; D012117:Resins, Synthetic / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet; D013678:Technology, Pharmaceutical / Q000379:methods*
chemical_list: D005419:Flavonoids; D012117:Resins, Synthetic; D000431:Ethanol