title: HIV infection of monocytes-derived dendritic cells inhibits Vγ9Vδ2 T cells functions.
abstract: DCs act as sentinel cells against incoming pathogens and represent the most potent antigen presenting cells, having the unique capability to prime naïve T cells. In addition to their role in induction of adaptive immune responses, DC are also able to activate innate cells as γδ T cells; in particular, a reciprocal crosstalk between DC and γδ T cells was demonstrated. However, whether HIV infection may alter DC-Vγ9Vδ2 T cells cross-talk was not yet described. To clarify this issue, we cultured activated Vγ9Vδ2 T cells with HIV infected monocyte derived DC (MoDC). After 5 days we evaluated MoDC phenotype, and Vγ9Vδ2 T cells activation and proliferation. In our model, Vγ9Vδ2 T cells were not able to proliferate in response to HIV-infected MoDC, although an up-regulation of CD69 was observed. Upon phosphoantigens stimulation, Vγ9Vδ2 T cells proliferation and cytokine production were inhibited when cultured with HIV-infected MoDC in a cell-contact dependent way. Moreover, HIV-infected MoDC are not able to up-regulate CD86 molecules when cultured with activated Vγ9Vδ2 T cells, compared with uninfected MoDC. Further, activated Vγ9Vδ2 T cells are not able to induce HLA DR up-regulation and CCR5 down-regulation on HIV-infected MoDC. These data indicate that HIV-infected DC alter the capacity of Vγ9Vδ2 T cells to respond to their antigens, pointing out a new mechanisms of induction of Vγ9Vδ2 T cells anergy carried out by HIV, that could contribute to immune evasion.
authors.affiliation: Laboratory of Cellular Immunology, National Institute for Infectious Diseases "Lazzaro Spallanzani", Rome, Italy.
authors.forename: Alessandra
authors.initials: A
authors.lastname: Sacchi
authors.affiliation: Laboratory of Cellular Immunology, National Institute for Infectious Diseases "Lazzaro Spallanzani", Rome, Italy.
authors.forename: Alessandra
authors.initials: A
authors.lastname: Rinaldi
authors.affiliation: Laboratory of Cellular Immunology, National Institute for Infectious Diseases "Lazzaro Spallanzani", Rome, Italy; Department of Biology, University of Rome "Tor Vergata", Rome, Italy.
authors.forename: Nicola
authors.initials: N
authors.lastname: Tumino
authors.affiliation: Laboratory of Cellular Immunology, National Institute for Infectious Diseases "Lazzaro Spallanzani", Rome, Italy.
authors.forename: Rita
authors.initials: R
authors.lastname: Casetti
authors.affiliation: Laboratory of Cellular Immunology, National Institute for Infectious Diseases "Lazzaro Spallanzani", Rome, Italy.
authors.forename: Chiara
authors.initials: C
authors.lastname: Agrati
authors.affiliation: Laboratory of Cellular Immunology, National Institute for Infectious Diseases "Lazzaro Spallanzani", Rome, Italy.
authors.forename: Federica
authors.initials: F
authors.lastname: Turchi
authors.affiliation: Laboratory of Cellular Immunology, National Institute for Infectious Diseases "Lazzaro Spallanzani", Rome, Italy.
authors.forename: Veronica
authors.initials: V
authors.lastname: Bordoni
authors.affiliation: Laboratory of Cellular Immunology, National Institute for Infectious Diseases "Lazzaro Spallanzani", Rome, Italy.
authors.forename: Eleonora
authors.initials: E
authors.lastname: Cimini
authors.affiliation: Laboratory of Cellular Immunology, National Institute for Infectious Diseases "Lazzaro Spallanzani", Rome, Italy.
authors.forename: Federico
authors.initials: F
authors.lastname: Martini
mesh_terms: D015703:Antigens, CD / Q000378:metabolism; D000945:Antigens, Differentiation, T-Lymphocyte / Q000378:metabolism; D051940:B7-2 Antigen / Q000378:metabolism; D049109:Cell Proliferation; D002478:Cells, Cultured; D018920:Coculture Techniques; D016207:Cytokines / Q000378:metabolism; D003713:Dendritic Cells / Q000166:cytology* / Q000821:virology; D015658:HIV Infections / Q000097:blood* / Q000276:immunology*; D015497:HIV-1; D006801:Humans; D007107:Immune System; D007113:Immunity, Innate; D037181:Lectins, C-Type / Q000378:metabolism; D008213:Lymphocyte Activation / Q000276:immunology; D009000:Monocytes / Q000166:cytology*; D010641:Phenotype; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000276:immunology*; D000099192:CD69 Antigens
chemical_list: D015703:Antigens, CD; D000945:Antigens, Differentiation, T-Lymphocyte; D051940:B7-2 Antigen; D016207:Cytokines; D037181:Lectins, C-Type; D016692:Receptors, Antigen, T-Cell, gamma-delta; D000099192:CD69 Antigens