title: Underlying mechanism of protection from hypoxic injury seen with n-butanol extract of Potentilla anserine L. in hippocampal neurons.
abstract: The alcohol and n-butanol extract of Potentilla anserine L. significantly protects myocardium from acute ischemic injury. However, its effects on rat hippocampal neurons and the mechanism of protection remain unclear. In this study, primary cultured hippocampal neurons from neonatal rats were incubated in 95% N2 and 5% CO2 for 4 hours. Results indicated that hypoxic injury decreased the viability of neurons, increased the expression levels of caspase-9 and caspase-3 mRNA, as well as cytochrome c, Caspase-9, and Caspase-3 protein. Pretreatment with 0.25, 0.062 5, 0.015 6 mg/mL n-butanol extract of Potentilla anserine L. led to a significant increase in cell viability. Expression levels of caspase-9 and caspase-3 mRNA, as well as cytochrome c, Caspase-9, and Caspase-3 protein, were attenuated. The neuroprotective effect of n-butanol extract of Potentilla anserine L. was equivalent to tanshinone IIA. Our data suggest that the n-butanol extract of Potentilla anserine L. could protect primary hippocampal neurons from hypoxic injury by deactivating mitochondrial cell death.
authors.affiliation: Department of Pathology, Affiliated Hospital of Logistics University of Chinese People's Armed Police Forces, Tianjin 300162, China.
authors.forename: Xiaojing
authors.initials: X
authors.lastname: Qin
authors.affiliation: Department of Medicinal Chemistry, Logistics University of Chinese People's Armed Police Forces, Tianjin 300162, China ; Tianjin Key Laboratory of Occupational and Environmental Hazard Biomarkers, Tianjin 300162, China.
authors.forename: Lingzhi
authors.initials: L
authors.lastname: Li
authors.affiliation: Department of Central Laboratory, Logistics University of Chinese People's Armed Police Forces, Tianjin 300162, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Lv
authors.affiliation: Department of Rescue Medicine, Logistics University of Chinese People's Armed Police Forces, Tianjin 300162, China.
authors.forename: Baoguo
authors.initials: B
authors.lastname: Yu
authors.affiliation: Department of Postgraduate, Logistics University of Chinese People's Armed Police Forces, Tianjin 300162, China.
authors.forename: Shuwang
authors.initials: S
authors.lastname: Yang
authors.affiliation: Department of Pathology, Affiliated Hospital of Logistics University of Chinese People's Armed Police Forces, Tianjin 300162, China.
authors.forename: Tao
authors.initials: T
authors.lastname: He
authors.affiliation: Tianjin Key Laboratory of Occupational and Environmental Hazard Biomarkers, Tianjin 300162, China ; Ministry of Scientific Research, Logistics University of Chinese People's Armed Police Forces, Tianjin 300162, China.
authors.forename: Yongliang
authors.initials: Y
authors.lastname: Zhang
keywords: caspase; cytochrome c; hypoxia; mitochondria injury; n-butanol extract of Potentilla anserine L; neural regeneration; neuron