title: Microfluidic antibody arrays for simultaneous cell separation and stimulus.
abstract: A microfluidic chip containing stamped antibody arrays was developed for simultaneous cell separation and drug testing. Poly(dimethyl siloxane) (PDMS) stamping was used to deposit antibodies in a microfluidic channel, forming discrete cell-capture regions on the surface. Cell mixtures were then introduced, resulting in the separation of cells when specific antibodies were used. Anti-CD19 antibody regions resulted in 94 % capture purity for CD19+ Ramos cells. An antibody that captures multiple cell types, for example anti-CD71, can also be used to capture several cell types simultaneously. Cells could also be loaded onto the arrays with spatial control using laminar streams. Both Ramos B cells and HuT 78 T cells were isolated in the chip and exposed to staurosporine in the same channel. Both cell lines had similar responses to the drug, with 2-10 % of cells remaining viable after 20 h of drug treatment, depending on cell type. The chip can also be used to analyze the efficacy of antibody therapy against cancer cells. Anti-CD95 was deposited on the surface and used for simultaneous cell capture and apoptosis induction via the extrinsic pathway. Cells captured on anti-CD95 surfaces had significant viability loss (15 % viability after 24 h) when compared with a control anti-CD71 antibody (81 % viability after 24 h). This chip can be used for a variety of cell separation and/or drug testing studies, enabling researchers to isolate cells and test them against different anti-cancer compounds and to follow cell response using fluorescence or other readout methods.
authors.affiliation: Department of Chemistry and Biochemistry, Texas Tech University, Lubbock, TX, 79409-1061, USA.
authors.forename: Yan
authors.initials: Y
authors.lastname: Liu
authors.forename: Todd
authors.initials: T
authors.lastname: Germain
authors.forename: Dimitri
authors.initials: D
authors.lastname: Pappas
mesh_terms: D000818:Animals; D055910:Antibodies, Immobilized / Q000737:chemistry*; D002460:Cell Line; D045744:Cell Line, Tumor; D002469:Cell Separation / Q000295:instrumentation*; D004354:Drug Screening Assays, Antitumor / Q000295:instrumentation; D004867:Equipment Design; D006801:Humans; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D040081:Protein Array Analysis / Q000295:instrumentation*; D059010:Single-Cell Analysis / Q000295:instrumentation*
chemical_list: D055910:Antibodies, Immobilized