title: WAVE binds Ena/VASP for enhanced Arp2/3 complex-based actin assembly.
abstract: The WAVE complex is the main activator of the Arp2/3 complex for actin filament nucleation and assembly in the lamellipodia of moving cells. Other important players in lamellipodial protrusion are Ena/VASP proteins, which enhance actin filament elongation. Here we examine the molecular coordination between the nucleating activity of the Arp2/3 complex and the elongating activity of Ena/VASP proteins for the formation of actin networks. Using an in vitro bead motility assay, we show that WAVE directly binds VASP, resulting in an increase in Arp2/3 complex-based actin assembly. We show that this interaction is important in vivo as well, for the formation of lamellipodia during the ventral enclosure event of Caenorhabditis elegans embryogenesis. Ena/VASP's ability to bind F-actin and profilin-complexed G-actin are important for its effect, whereas Ena/VASP tetramerization is not necessary. Our data are consistent with the idea that binding of Ena/VASP to WAVE potentiates Arp2/3 complex activity and lamellipodial actin assembly.
authors.affiliation: Institut Curie, Centre de Recherche Centre National de la Recherche Scientifique, Unité Mixte de Recherche 168 Université Pierre et Marie Curie, Paris F-75248, France.
authors.forename: Svitlana
authors.initials: S
authors.lastname: Havrylenko
authors.affiliation: Institut Curie, Centre de Recherche Centre National de la Recherche Scientifique, Unité Mixte de Recherche 168 Université Pierre et Marie Curie, Paris F-75248, France.
authors.forename: Philippe
authors.initials: P
authors.lastname: Noguera
authors.affiliation: Institut Curie, Centre de Recherche Centre National de la Recherche Scientifique, Unité Mixte de Recherche 168 Université Pierre et Marie Curie, Paris F-75248, France.
authors.forename: Majdouline
authors.initials: M
authors.lastname: Abou-Ghali
authors.affiliation: Institut Curie, Centre de Recherche Centre National de la Recherche Scientifique, Unité Mixte de Recherche 168 Université Pierre et Marie Curie, Paris F-75248, France.
authors.forename: John
authors.initials: J
authors.lastname: Manzi
authors.affiliation: Institut Curie, Centre de Recherche Centre National de la Recherche Scientifique, Unité Mixte de Recherche 168 Université Pierre et Marie Curie, Paris F-75248, France.
authors.forename: Fahima
authors.initials: F
authors.lastname: Faqir
authors.affiliation: Institut Curie, Centre de Recherche Centre National de la Recherche Scientifique, Unité Mixte de Recherche 168 Université Pierre et Marie Curie, Paris F-75248, France.
authors.forename: Audrey
authors.initials: A
authors.lastname: Lamora
authors.affiliation: Laboratoire de Physiologie Cellulaire et Végétale, Institut de Recherches en Technologies et Sciences pour le Vivant, CNRS/CEA/INRA/UJF, Grenoble 38054, France.
authors.forename: Christophe
authors.initials: C
authors.lastname: Guérin
authors.affiliation: Laboratoire de Physiologie Cellulaire et Végétale, Institut de Recherches en Technologies et Sciences pour le Vivant, CNRS/CEA/INRA/UJF, Grenoble 38054, France.
authors.forename: Laurent
authors.initials: L
authors.lastname: Blanchoin
authors.affiliation: Institut Curie, Centre de Recherche Centre National de la Recherche Scientifique, Unité Mixte de Recherche 168 Université Pierre et Marie Curie, Paris F-75248, France julie.plastino@curie.fr.
authors.forename: Julie
authors.initials: J
authors.lastname: Plastino
mesh_terms: D008841:Actin Cytoskeleton / Q000737:chemistry*; D051376:Actin-Related Protein 2-3 Complex / Q000737:chemistry*; D000199:Actins / Q000737:chemistry*; D000818:Animals; D017173:Caenorhabditis elegans; D029742:Caenorhabditis elegans Proteins / Q000737:chemistry*; D002465:Cell Movement; D004268:DNA-Binding Proteins / Q000737:chemistry*; D047108:Embryonic Development; D006801:Humans; D051304:Profilins / Q000737:chemistry; D011554:Pseudopodia; D051300:Wiskott-Aldrich Syndrome Protein Family / Q000737:chemistry*
chemical_list: D051376:Actin-Related Protein 2-3 Complex; D000199:Actins; D029742:Caenorhabditis elegans Proteins; D004268:DNA-Binding Proteins; C092744:ENA-VASP proteins; D051304:Profilins; C114864:WASF1 protein, human; D051300:Wiskott-Aldrich Syndrome Protein Family; C532811:wve-1 protein, C elegans