title: The role of visfatin on the regulation of inflammation and apoptosis in the spleen of LPS-treated rats.
abstract: The purpose of the present study is to determine if visfatin is involved in inflammation or apoptosis induced by LPS in rat. Forty Wistar rats were divided into four groups: saline group, LPS group, visfatin group and Visfatin + LPS co-stimulated group. Spleen samples from each group of rats were collected for study. The spleen structure was examined by histological imaging. Apoptosis was evaluated with TUNEL reaction. Caspase-3 was detected with immunohistochemistry and western blot. The apoptosis-related genes were detected by qPCR and inflammatory cytokines were tested by ELISA. Our main findings were as follows. (1) Macrophages were markedly increased in the visfatin group compared with the saline group. This finding was confirmed when spleen samples were examined with western blot using CD68 antibody. (2) Visfatin promoted the expression of CD68 and caspase-3 in rat spleen, whereas visfatin could inhibit the expression of CD68 and activated caspase-3 in spleen of LPS-induced acute inflammation. (3) Visfatin had a pro-apoptotic effect on normal rat spleen, whereas it exerted an anti-apoptotic effect during LPS-induced lymphocytes apoptosis in rat spleen. Moreover, the effect of visfatin on cell apoptosis was mediated by the mitochondrial pathway. (4) Visfatin could modulate both the anti-inflammatory cytokines and pro-inflammatory cytokines in rat spleen, such as IL-10, IL-4, IL-6, TNF-α and IL-1β. Taken together, we demonstrate that visfatin could participate in the inflammatory process in rat spleen by modulating the macrophages and inflammatory cytokines. Also, visfatin plays a dual role in the apoptosis in rat spleen, which is mediated by the mitochondrial pathway.
authors.affiliation: College of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, China.
authors.forename: Ke
authors.initials: K
authors.lastname: Xiao
authors.affiliation: College of Plant Science & Technology, Huazhong Agricultural University, Wuhan, 430070, China.
authors.forename: Wei-Hua
authors.initials: WH
authors.lastname: Zou
authors.affiliation: College of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, China.
authors.forename: Zhi
authors.initials: Z
authors.lastname: Yang
authors.affiliation: College of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, China.
authors.forename: Zia Ur
authors.initials: ZU
authors.lastname: Rehman
authors.affiliation: College of Veterinary and Animal Sciences, Jhang University of Veterinary and Animal Sciences, Lahore, Pakistan.
authors.forename: Abdur Rahman
authors.initials: AR
authors.lastname: Ansari
authors.affiliation: College of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, China.
authors.forename: Huai-Rui
authors.initials: HR
authors.lastname: Yuan
authors.affiliation: College of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: College of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Cui
authors.affiliation: College of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, China.
authors.forename: Ke-Mei
authors.initials: KM
authors.lastname: Peng
authors.affiliation: College of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, China. songh2007@mail.hzau.edu.cn.
authors.forename: Hui
authors.initials: H
authors.lastname: Song
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000378:metabolism; D015214:Antigens, Differentiation, Myelomonocytic / Q000378:metabolism; D017209:Apoptosis; D053148:Caspase 3 / Q000235:genetics / Q000378:metabolism; D016207:Cytokines / Q000378:metabolism; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay; D007150:Immunohistochemistry; D007249:Inflammation / Q000473:pathology*; D018836:Inflammation Mediators / Q000378:metabolism; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008214:Lymphocytes / Q000187:drug effects / Q000378:metabolism; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism; D054409:Nicotinamide Phosphoribosyltransferase / Q000378:metabolism*; D017208:Rats, Wistar; D015398:Signal Transduction / Q000187:drug effects; D013154:Spleen / Q000187:drug effects / Q000201:enzymology* / Q000473:pathology*; D051028:bcl-2-Associated X Protein / Q000235:genetics / Q000378:metabolism; D000097382:CD68 Molecule
chemical_list: D015703:Antigens, CD; D015214:Antigens, Differentiation, Myelomonocytic; D053148:Caspase 3; D016207:Cytokines; D018836:Inflammation Mediators; D008070:Lipopolysaccharides; D054409:Nicotinamide Phosphoribosyltransferase; D051028:bcl-2-Associated X Protein; C067980:CD68 antigen, human; D000097382:CD68 Molecule