title: Protective effects of Arctium lappa L. roots against hydrogen peroxide-induced cell injury and potential mechanisms in SH-SY5Y cells.
abstract: Accumulated evidence has shown that excessive reactive oxygen species (ROS) have been implicated in neuronal cell death related with various chronic neurodegenerative disorders. This study was designed to explore neuroprotective effects of ethyl acetate extract of Arctium lappa L. roots (EAL) on hydrogen peroxide (H2O2)-induced cell injury in human SH-SY5Y neuroblastoma cells. The cell viability was significantly decreased after exposure to 200 μM H2O2, whereas pretreatment with different concentrations of EAL attenuated the H2O2-induced cytotoxicity. Hoechst 33342 staining indicated that EAL reversed nuclear condensation in H2O2-treated cells. Meanwhile, TUNEL assay with DAPI staining showed that EAL attenuated apoptosis was induced by H2O2. Pretreatment with EAL also markedly elevated activities of antioxidant enzyme (GSH-Px and SOD), reduced lipid peroxidation (MDA) production, prevented ROS formation, and the decrease of mitochondrial membrane potential. In addition, EAL showed strong radical scavenging ability in 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) assays. Furthermore, EAL inhibited H2O2-induced apoptosis by increases in the Bcl-2/Bax ratio, decreases in cytochrome c release, and attenuation of caspase-3, caspase-9 activities, and expressions. These findings suggest that EAL may be regarded as a potential antioxidant agent and possess potent neuroprotective activity against H2O2-induced injury.
authors.affiliation: Department of Pharmacy, General Hospital of Shenyang Military Area Command, Shenyang, 110840, China.|Department of Life Science and Biochemistry, Shenyang Pharmaceutical University, Shenyang, 110016, China.
authors.forename: Xing
authors.initials: X
authors.lastname: Tian
authors.affiliation: Department of Pharmacy, General Hospital of Shenyang Military Area Command, Shenyang, 110840, China.
authors.forename: Li-Ping
authors.initials: LP
authors.lastname: Guo
authors.affiliation: Department of Pharmacy, General Hospital of Shenyang Military Area Command, Shenyang, 110840, China.
authors.forename: Xiao-Long
authors.initials: XL
authors.lastname: Hu
authors.affiliation: Department of Pharmacy, General Hospital of Shenyang Military Area Command, Shenyang, 110840, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Huang
authors.affiliation: Department of Pharmacy, General Hospital of Shenyang Military Area Command, Shenyang, 110840, China.
authors.forename: Yan-Hua
authors.initials: YH
authors.lastname: Fan
authors.affiliation: Department of Pharmacy, General Hospital of Shenyang Military Area Command, Shenyang, 110840, China.
authors.forename: Tian-Shu
authors.initials: TS
authors.lastname: Ren
authors.affiliation: Department of Pharmacy, General Hospital of Shenyang Military Area Command, Shenyang, 110840, China. zhaoqingchun1967@163.com.|Department of Life Science and Biochemistry, Shenyang Pharmaceutical University, Shenyang, 110016, China. zhaoqingchun1967@163.com.
authors.forename: Qing-Chun
authors.initials: QC
authors.lastname: Zhao
mesh_terms: D000975:Antioxidants / Q000302:isolation & purification / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects / Q000502:physiology; D031170:Arctium; D016923:Cell Death / Q000187:drug effects / Q000502:physiology; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D004305:Dose-Response Relationship, Drug; D006801:Humans; D006861:Hydrogen Peroxide / Q000037:antagonists & inhibitors / Q000633:toxicity*; D018384:Oxidative Stress / Q000187:drug effects / Q000502:physiology; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D018517:Plant Roots; D020011:Protective Agents / Q000302:isolation & purification / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; D010936:Plant Extracts; D020011:Protective Agents; D006861:Hydrogen Peroxide