title: Biocompatibility of tungsten disulfide inorganic nanotubes and fullerene-like nanoparticles with salivary gland cells.
abstract: Impaired salivary gland (SG) function leading to oral diseases is relatively common with no adequate solution. Previously, tissue engineering of SG had been proposed to overcome this morbidity, however, not yet clinically available. Multiwall inorganic (tungsten disulfide [WS2]) nanotubes (INT-WS2) and fullerene-like nanoparticles (IF-WS2) have many potential medical applications. A yet unexplored venue application is their interaction with SG, and therefore, our aim was to test the biocompatibility of INT/IF-WS2 with the A5 and rat submandibular cells (RSC) SG cells. The cells were cultured and subjected after 1 day to different concentrations of INT-WS2 and were compared to control groups. Growth curves, trypan blue viability test, and carboxyfluorescein succinimidyl ester (CFSE) proliferation assay were obtained. Furthermore, cells morphology and interaction with the nanoparticles were observed by light microscopy, scanning electron microscopy and transmission electron microscopy (TEM), and energy dispersive X-ray spectroscopy. The results showed no significant differences in growth curves, proliferation kinetics, and viability between the groups compared. Moreover, no alterations were observed in the cell morphology. Interestingly, TEM images indicated that the nanoparticles are uptaken by the cells and accumulate in cytoplasmic vesicles. These results suggest promising future medical applications for these nanoparticles.
authors.affiliation: 1 Faculty of Dental Medicine, The Hebrew University of Jerusalem , Jerusalem, Israel .
authors.forename: Elisheva B
authors.initials: EB
authors.lastname: Goldman
authors.forename: Alla
authors.initials: A
authors.lastname: Zak
authors.forename: Reshef
authors.initials: R
authors.lastname: Tenne
authors.forename: Elena
authors.initials: E
authors.lastname: Kartvelishvily
authors.forename: Smadar
authors.initials: S
authors.lastname: Levin-Zaidman
authors.forename: Yoav
authors.initials: Y
authors.lastname: Neumann
authors.forename: Raluca
authors.initials: R
authors.lastname: Stiubea-Cohen
authors.forename: Aaron
authors.initials: A
authors.lastname: Palmon
authors.forename: Avi-Hai
authors.initials: AH
authors.lastname: Hovav
authors.forename: Doron J
authors.initials: DJ
authors.lastname: Aframian
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D037741:Fullerenes / Q000494:pharmacology*; D008422:Materials Testing / Q000379:methods*; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D043942:Nanotubes / Q000737:chemistry* / Q000648:ultrastructure; D051381:Rats; D013363:Submandibular Gland / Q000166:cytology* / Q000187:drug effects / Q000648:ultrastructure; D013440:Sulfides / Q000494:pharmacology*; D017973:Tungsten Compounds / Q000494:pharmacology*
chemical_list: D001672:Biocompatible Materials; D037741:Fullerenes; D013440:Sulfides; D017973:Tungsten Compounds