title: Cofactor metals and antioxidant enzymes in cisplatin-treated rats: effect of antioxidant intervention.
abstract: We explored the association between the activities of antioxidant enzymes and their metallic cofactors in rats treated with cisplatin. The antioxidant effects of aminoguanidine, and a combination of vitamins E and C were investigated. Plasma platin was significantly lower than liver and kidney. Cisplatin treatment caused significant increase in plasma Se-glutathione peroxidase activity. Activities of Se-glutathione peroxidase, glutathione S-transferase, catalase and Cu,Zn-superoxide dismutase have been found to be significantly decreased in liver and kidney compared to controls. Zn levels in these organs were diminished upon cisplatin treatment, while levels of Cu were unaffected. Interestingly, levels of iron, the cofactor of catalase, were found to be significantly increased in liver and kidney. Intervention with aminoguanidine or vitamins was generally prevented cisplatin-caused changes in the activity of enzymes and in the tissue levels of cofactor metals. These observations suggest that relation between activities of enzymes and levels of cofactor metals is multifactorial.
authors.affiliation: a Department of Toxicology, Faculty of Pharmacy , Hacettepe University , Ankara , Turkey .
authors.forename: Suna
authors.initials: S
authors.lastname: Sabuncuoglu
authors.affiliation: b Department of Toxicology, Faculty of Pharmacy , Erciyes University , Kayseri , Turkey .
authors.forename: Ayse
authors.initials: A
authors.lastname: Eken
authors.affiliation: c Department of Toxicology, Faculty of Pharmacy , Yeditepe University , Istanbul , Turkey , and.
authors.forename: Ahmet
authors.initials: A
authors.lastname: Aydin
authors.affiliation: a Department of Toxicology, Faculty of Pharmacy , Hacettepe University , Ankara , Turkey .
authors.forename: Hilal
authors.initials: H
authors.lastname: Ozgunes
authors.affiliation: d Department of Toxicology, Faculty of Pharmacy , Ege University , Izmir , Turkey.
authors.forename: Hilmi
authors.initials: H
authors.lastname: Orhan
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000633:toxicity; D000975:Antioxidants / Q000008:administration & dosage / Q000378:metabolism* / Q000494:pharmacology; D001205:Ascorbic Acid / Q000008:administration & dosage / Q000494:pharmacology; D002374:Catalase / Q000378:metabolism; D002945:Cisplatin / Q000633:toxicity*; D003067:Coenzymes / Q000737:chemistry / Q000378:metabolism*; D004798:Enzymes / Q000378:metabolism*; D005979:Glutathione Peroxidase / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism; D006146:Guanidines / Q000008:administration & dosage / Q000494:pharmacology; D007668:Kidney / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008297:Male; D008670:Metals / Q000737:chemistry / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013482:Superoxide Dismutase / Q000378:metabolism; D014810:Vitamin E / Q000008:administration & dosage / Q000494:pharmacology
chemical_list: D000970:Antineoplastic Agents; D000975:Antioxidants; D003067:Coenzymes; D004798:Enzymes; D006146:Guanidines; D008670:Metals; D014810:Vitamin E; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005982:Glutathione Transferase; D001205:Ascorbic Acid; D002945:Cisplatin; C004479:pimagedine
keywords: Antioxidant enzymes; cisplatin; in vivo; metallic cofactors; rat