title: Flavobacterium suzhouense sp. nov., isolated from farmland river sludge.
abstract: A Gram-stain-negative bacterium, designated XIN-1(T), was isolated from a farmland river sludge sample in Suzhou, China. Cells of strain XIN-1(T) were strictly aerobic, non-motile and rod-shaped. Strain XIN-1(T) grew optimally at pH 7.0 and 28 °C. Phylogenetic analysis of the 16S rRNA gene sequences showed that strain XIN-1(T) was most closely related to Flavobacterium hauense BX12(T) (98.2 % sequence similarity), followed by Flavobacterium beibuense F44-8(T) (96.3 %). The major respiratory quinone was menaquinone-6 and the major polar lipid was phosphatidylethanolamine. The major fatty acids (>5 %) were summed feature 3 (comprising C16 : 1ω7c and/or C16 : 1ω6c), summed feature 4 (comprising iso-C17 : 1 I and/or anteiso-C17 : 1 B), iso-C15 : 0, C16 : 0 and iso-C17 : 0 3-OH. The genomic DNA G+C content of strain XIN-1(T) was 39.8 mol%. Strain XIN-1(T) showed low DNA-DNA relatedness with F. hauense BX12(T) (38.7±0.5 %). On the basis of genotypic and phenotypic data, strain XIN-1(T) is considered to represent a novel species of the genus Flavobacterium, for which the name Flavobacterium suzhouense sp. nov. is proposed. The type strain is XIN-1(T) ( = CCTCC AB 2014200(T) = KCTC 42107(T)).
authors.affiliation: Key Laboratory of Microbiology of Agricultural Environment, Ministry of Agriculture, College of life sciences, Nanjing Agricultural University, Nanjing, 210095, PR China.
authors.forename: Hao
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Microbiology of Agricultural Environment, Ministry of Agriculture, College of life sciences, Nanjing Agricultural University, Nanjing, 210095, PR China.
authors.forename: Ming-Gen
authors.initials: MG
authors.lastname: Cheng
authors.affiliation: Key Laboratory of Microbiology of Agricultural Environment, Ministry of Agriculture, College of life sciences, Nanjing Agricultural University, Nanjing, 210095, PR China.
authors.forename: Bin
authors.initials: B
authors.lastname: Sun
authors.affiliation: Key Laboratory of Microbiology of Agricultural Environment, Ministry of Agriculture, College of life sciences, Nanjing Agricultural University, Nanjing, 210095, PR China.
authors.forename: Su-Hui
authors.initials: SH
authors.lastname: Guo
authors.affiliation: Key Laboratory of Microbiology of Agricultural Environment, Ministry of Agriculture, College of life sciences, Nanjing Agricultural University, Nanjing, 210095, PR China.
authors.forename: Man
authors.initials: M
authors.lastname: Song
authors.affiliation: Key Laboratory of Microbiology of Agricultural Environment, Ministry of Agriculture, College of life sciences, Nanjing Agricultural University, Nanjing, 210095, PR China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Li
authors.affiliation: Key Laboratory of Microbiology of Agricultural Environment, Ministry of Agriculture, College of life sciences, Nanjing Agricultural University, Nanjing, 210095, PR China.
authors.forename: Xing
authors.initials: X
authors.lastname: Huang
mesh_terms: D000383:Agriculture; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D005417:Flavobacterium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010714:Phosphatidylethanolamines / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D045483:Rivers / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D012722:Sewage / Q000382:microbiology*; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010714:Phosphatidylethanolamines; D012336:RNA, Ribosomal, 16S; D012722:Sewage; D024482:Vitamin K 2; C483858:phosphatidylethanolamine; C042455:menaquinone 6