title: Expression profiling of selected glutathione transferase genes in Zea mays (L.) seedlings infested with cereal aphids.
abstract: The purpose of this report was to evaluate the expression patterns of selected glutathione transferase genes (gst1, gst18, gst23 and gst24) in the tissues of two maize (Zea mays L.) varieties (relatively resistant Ambrozja and susceptible Tasty Sweet) that were colonized with oligophagous bird cherry-oat aphid (Rhopalosiphum padi L.) or monophagous grain aphid (Sitobion avenae L.). Simultaneously, insect-triggered generation of superoxide anion radicals (O2•-) in infested Z. mays plants was monitored. Quantified parameters were measured at 1, 2, 4, 8, 24, 48 and 72 h post-initial aphid infestation (hpi) in relation to the non-infested control seedlings. Significant increases in gst transcript amounts were recorded in aphid-stressed plants in comparison to the control seedlings. Maximal enhancement in the expression of the gst genes in aphid-attacked maize plants was found at 8 hpi (gst23) or 24 hpi (gst1, gst18 and gst24) compared to the control. Investigated Z. mays cultivars formed excessive superoxide anion radicals in response to insect treatments, and the highest overproduction of O2•- was noted 4 or 8 h after infestation, depending on the aphid treatment and maize genotype. Importantly, the Ambrozja variety could be characterized as having more profound increments in the levels of gst transcript abundance and O2•- generation in comparison with the Tasty Sweet genotype.
authors.affiliation: Siedlce University of Natural Sciences and Humanities, Department of Biochemistry and Molecular Biology, Siedlce, Poland.
authors.forename: Hubert
authors.initials: H
authors.lastname: Sytykiewicz
authors.affiliation: Siedlce University of Natural Sciences and Humanities, Department of Biochemistry and Molecular Biology, Siedlce, Poland.
authors.forename: Grzegorz
authors.initials: G
authors.lastname: Chrzanowski
authors.affiliation: Siedlce University of Natural Sciences and Humanities, Department of Biochemistry and Molecular Biology, Siedlce, Poland.
authors.forename: Paweł
authors.initials: P
authors.lastname: Czerniewicz
authors.affiliation: Siedlce University of Natural Sciences and Humanities, Department of Biochemistry and Molecular Biology, Siedlce, Poland.
authors.forename: Iwona
authors.initials: I
authors.lastname: Sprawka
authors.affiliation: Siedlce University of Natural Sciences and Humanities, Department of Biochemistry and Molecular Biology, Siedlce, Poland.
authors.forename: Iwona
authors.initials: I
authors.lastname: Łukasik
authors.affiliation: Siedlce University of Natural Sciences and Humanities, Department of Biochemistry and Molecular Biology, Siedlce, Poland.
authors.forename: Sylwia
authors.initials: S
authors.lastname: Goławska
authors.affiliation: Siedlce University of Natural Sciences and Humanities, Department of Biochemistry and Molecular Biology, Siedlce, Poland.
authors.forename: Cezary
authors.initials: C
authors.lastname: Sempruch
mesh_terms: D000818:Animals; D001042:Aphids; D015971:Gene Expression Regulation, Enzymologic; D018506:Gene Expression Regulation, Plant; D005982:Glutathione Transferase / Q000096:biosynthesis*; D010935:Plant Diseases / Q000469:parasitology*; D010940:Plant Proteins / Q000096:biosynthesis*; D036226:Seedlings / Q000201:enzymology / Q000469:parasitology; D003313:Zea mays / Q000201:enzymology / Q000469:parasitology
chemical_list: D010940:Plant Proteins; D005982:Glutathione Transferase