title: Evaluation of the efficacy of outer membrane protein 31 vaccine formulations for protection against Brucella canis in BALB/c mice.
abstract: Canine brucellosis is an infectious disease caused by the Gram-negative bacterium Brucella canis. Unlike conventional control programs for other species of the genus Brucella, currently there is no vaccine available against canine brucellosis, and preventive measures are simply diagnosis and isolation of infected dogs. New approaches are therefore needed to develop an effective and safe immunization strategy against this zoonotic pathogen. In this study, BALB/c mice were subcutaneously immunized with the following: (i) the recombinant Brucella Omp31 antigen formulated in different adjuvants (incomplete Freund adjuvant, aluminum hydroxide, Quil A, and Montanide IMS 3012 VGPR), (ii) plasmid pCIOmp31, or (iii) pCIOmp31 plasmid followed by boosting with recombinant Omp31 (rOmp31). The immune response and the protective efficacy against B. canis infection were characterized. The different strategies induced a strong immunoglobulin G (IgG) response. Furthermore, spleen cells from rOmp31-immunized mice produced gamma interferon and interleukin-4 (IL-4) after in vitro stimulation with rOmp31, indicating the induction of a mixed Th1-Th2 response. Recombinant Omp31 administered with different adjuvants as well as the prime-boost strategy conferred protection against B. canis. In conclusion, our results suggest that Omp31 could be a useful candidate for the development of a subcellular vaccine against B. canis infection.
authors.affiliation: Laboratorio de Inmunología, Departamento SAMP, Facultad de Ciencias Veterinarias, Centro de Investigación Veterinaria de Tandil (CIVETAN-CONICET), Universidad Nacional del Centro de la Provincia de Buenos Aires, Tandil, Argentina.
authors.forename: Maria
authors.initials: M
authors.lastname: Clausse
authors.affiliation: Laboratorio de Inmunología, Departamento SAMP, Facultad de Ciencias Veterinarias, Centro de Investigación Veterinaria de Tandil (CIVETAN-CONICET), Universidad Nacional del Centro de la Provincia de Buenos Aires, Tandil, Argentina.
authors.forename: Alejandra G
authors.initials: AG
authors.lastname: Díaz
authors.affiliation: Instituto de Inmunología, Genética y Metabolismo (CONICET/UBA), Buenos Aires, Argentina.
authors.forename: Andrés E
authors.initials: AE
authors.lastname: Ibañez
authors.affiliation: Instituto de Inmunología, Genética y Metabolismo (CONICET/UBA), Buenos Aires, Argentina.
authors.forename: Juliana
authors.initials: J
authors.lastname: Cassataro
authors.affiliation: Instituto de Inmunología, Genética y Metabolismo (CONICET/UBA), Buenos Aires, Argentina.
authors.forename: Guillermo H
authors.initials: GH
authors.lastname: Giambartolomei
authors.affiliation: Laboratorio de Inmunología, Departamento SAMP, Facultad de Ciencias Veterinarias, Centro de Investigación Veterinaria de Tandil (CIVETAN-CONICET), Universidad Nacional del Centro de la Provincia de Buenos Aires, Tandil, Argentina silmares@vet.unicen.edu.ar.
authors.forename: Silvia M
authors.initials: SM
authors.lastname: Estein
mesh_terms: D000276:Adjuvants, Immunologic / Q000008:administration & dosage; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood*; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology*; D002004:Brucella Vaccine / Q000276:immunology*; D041002:Brucella canis / Q000276:immunology*; D002006:Brucellosis / Q000276:immunology* / Q000517:prevention & control; D004285:Dogs; D051379:Mice; D008807:Mice, Inbred BALB C; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D014611:Vaccination
chemical_list: D000276:Adjuvants, Immunologic; D000907:Antibodies, Bacterial; D001425:Bacterial Outer Membrane Proteins; D002004:Brucella Vaccine