title: Quantitative analysis on collagen of dermatofibrosarcoma protuberans skin by second harmonic generation microscopy.
abstract: Dermatofibrosarcoma protuberans (DFSP) is a skin cancer usually mistaken as other benign tumors. Abnormal DFSP resection results in tumor recurrence. Quantitative characterization of collagen alteration on the skin tumor is essential for developing a diagnostic technique. In this study, second harmonic generation (SHG) microscopy was performed to obtain images of the human DFSP skin and normal skin. Subsequently, structure and texture analysis methods were applied to determine the differences in skin texture characteristics between the two skin types, and the link between collagen alteration and tumor was established. Results suggest that combining SHG microscopy and texture analysis methods is a feasible and effective method to describe the characteristics of skin tumor like DFSP.
authors.affiliation: Key Lab of OptoElectronic Science and Technology for Medicine, Ministry of Education, Fujian Provincial Key Lab of Photonic Technology, College of Photonic and Electronic Engineering, Fujian Normal University, Fuzhou, Fujian, China.
authors.forename: Shulian
authors.initials: S
authors.lastname: Wu
authors.forename: Yudian
authors.initials: Y
authors.lastname: Huang
authors.forename: Hui
authors.initials: H
authors.lastname: Li
authors.forename: Yunxia
authors.initials: Y
authors.lastname: Wang
authors.forename: Xiaoman
authors.initials: X
authors.lastname: Zhang
mesh_terms: D003094:Collagen / Q000032:analysis*; D018223:Dermatofibrosarcoma / Q000175:diagnosis / Q000473:pathology*; D006801:Humans; D008853:Microscopy / Q000379:methods*; D010336:Pathology / Q000379:methods; D012867:Skin / Q000473:pathology*; D012878:Skin Neoplasms / Q000175:diagnosis / Q000473:pathology*
chemical_list: D003094:Collagen
keywords: Dermatofibrosarcoma protuberans skin; second generation microscopy; texture feature