title: Structural basis of multivalent galactose-based dendrimer recognition by human galectin-7.
abstract: Galectins are evolutionarily conserved and ubiquitously present animal lectins with a high affinity for β-galactose-containing oligosaccharides. To date, 15 mammalian galectins have been identified. Their involvement in cell-cell and cell-matrix interactions has highlighted their importance in signal transduction and other intracellular processes. Human galectin-7 (hGal-7) is a 15 kDa proto type galectin that forms a dimer in solution and its involvement in the stimulation and development of tumour growth has been reported. Previously, we reported the crystal structure of hGal-7 and its complex with galactose and lactose which provided insight into its molecular recognition and detailed interactions. Here, we present newly obtained high-resolution structural data on carbohydrate-based dendrons in complex with hGal-7. Our crystallographic data reveal how multivalent ligands interact with and form cross-links with these galectin molecules. Understanding how these dendrimeric compounds interact with hGal-7 would help in the design of new tools to investigate the recognition of carbohydrates by lectins.
authors.affiliation: Department of Biology and Biochemistry, University of Bath, UK.
authors.forename: Sneha
authors.initials: S
authors.lastname: Ramaswamy
authors.forename: Mazen H
authors.initials: MH
authors.lastname: Sleiman
authors.forename: Geoffrey
authors.initials: G
authors.lastname: Masuyer
authors.forename: Cécile
authors.initials: C
authors.lastname: Arbez-Gindre
authors.forename: Maria
authors.initials: M
authors.lastname: Micha-Screttas
authors.forename: Theodora
authors.initials: T
authors.lastname: Calogeropoulou
authors.forename: Barry R
authors.initials: BR
authors.lastname: Steele
authors.forename: K Ravi
authors.initials: KR
authors.lastname: Acharya
mesh_terms: D000818:Animals; D001665:Binding Sites; D018360:Crystallography, X-Ray; D050091:Dendrimers / Q000737:chemistry; D019281:Dimerization; D005690:Galactose / Q000737:chemistry* / Q000378:metabolism; D037161:Galectins / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D007785:Lactose / Q000737:chemistry*; D008024:Ligands; D009844:Oligosaccharides / Q000737:chemistry*; D017434:Protein Structure, Tertiary; D017386:Sequence Homology, Amino Acid
chemical_list: D050091:Dendrimers; D037161:Galectins; C092525:LGALS7 protein, human; D008024:Ligands; D009844:Oligosaccharides; D007785:Lactose; D005690:Galactose
keywords: carbohydrate binding; dendrimers; galectin-7; lectin; multivalency