title: Culturing Drosophila melanogaster (S2) in a chemostat.
abstract: Insect cells are used for the expression of complex proteins in products such as vaccines and biopharmaceuticals. Physiology of a Drosophila melanogaster (lineage S2), transfected to stably express rabies virus glycoprotein (RVGP), was studied in batch culture and in a chemostat with serum-free medium. In batch mode, the system reached 3 × 10(7) cells mL(-1) with specific growth rate of 1.5 d(-1) with RVGP at 2.50 µg L(-1). When operated continuously, three well-defined steady states were achieved at dilution rates (D) of 0.8, 0.5 and 0.2 d(-1). The residual glucose and glutamine concentrations and the cell yields on glucose and glutamine decreased at lower D. High values of substrate consumption for maintenance may explain this variation in yields. The results indicated that glucose is not the limiting substrate of this process.
authors.affiliation: Laboratório de Células Animais, Escola Politécnica--Universidade de São Paulo, P.O. Box 61.548, São Paulo, SP, CEP 05524-970, Brazil, paulabruzadelle@gmail.com.
authors.forename: Paula Bruzadelle
authors.initials: PB
authors.lastname: Vieira
authors.forename: Bruno Labate Vale
authors.initials: BL
authors.lastname: da Costa
authors.forename: Elisabeth
authors.initials: E
authors.lastname: de Fatima Pires Augusto
authors.forename: Aldo
authors.initials: A
authors.lastname: Tonso
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques; D002460:Cell Line; D016895:Culture Media, Serum-Free / Q000737:chemistry; D004331:Drosophila melanogaster; D005947:Glucose / Q000032:analysis; D005973:Glutamine / Q000032:analysis; D006023:Glycoproteins / Q000235:genetics / Q000378:metabolism*; D011820:Rabies virus / Q000235:genetics*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D016895:Culture Media, Serum-Free; D006023:Glycoproteins; D011994:Recombinant Proteins; D014764:Viral Proteins; D005973:Glutamine; D005947:Glucose