title: Use of an acidophilic yeast strain to enable the growth of leaching bacteria on solid media.
abstract: In this study, a Candida digboiensis strain was isolated from a heap leaching plant in Zambia and used in double-layer agar plate to efficiently isolate and purify leaching bacteria. Unlike Acidiphilium sp., the yeast strain was tetrathionate tolerant and could metabolize a great range of organic compounds including organic acids. These properties allowed the yeast strain to enable and fasten the growth of iron and sulfur oxidizers on double-layer agar plate. The isolates were identified as Acidithiobacillus ferrooxidans FOX1, Leptospirillun ferriphilum BN, and Acidithiobacillus thiooxidans ZMB. These three leaching bacteria were inhibited by organic acids such as acetic and propionic acids; however, their activities were enhanced by Candida digboiensis NB under dissolved organic matter stress.
authors.affiliation: School of Minerals Processing and Bioengineering, Central South University, Changsha, 410083, Hunan, China, ngom-baba@hotmail.com.
authors.forename: Baba
authors.initials: B
authors.lastname: Ngom
authors.forename: Yili
authors.initials: Y
authors.lastname: Liang
authors.forename: Yi
authors.initials: Y
authors.lastname: Liu
authors.forename: Huaqun
authors.initials: H
authors.lastname: Yin
authors.forename: Xueduan
authors.initials: X
authors.lastname: Liu
mesh_terms: D019342:Acetic Acid / Q000494:pharmacology; D041801:Acidiphilium / Q000254:growth & development / Q000378:metabolism; D042763:Acidithiobacillus / Q000145:classification / Q000187:drug effects / Q000235:genetics / Q000254:growth & development* / Q000648:ultrastructure; D013856:Acidithiobacillus thiooxidans / Q000145:classification / Q000187:drug effects / Q000235:genetics / Q000254:growth & development / Q000648:ultrastructure; D002175:Candida / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D046508:Culture Techniques; D007501:Iron / Q000378:metabolism; D056265:Microbial Interactions; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D013457:Sulfur Compounds / Q000378:metabolism
chemical_list: D012336:RNA, Ribosomal, 16S; D012337:RNA, Ribosomal, 18S; D013457:Sulfur Compounds; D007501:Iron; D019342:Acetic Acid