title: Development of an immunochromatographic strip test for the rapid detection of zearalenone in corn.
abstract: A rapid immunochromatographic test strip has been developed for the detection of zearalenone (ZEN) residues in corn. For this purpose, a specific anti-ZEN monoclonal antibody (mAb), 4A3-F9, was obtained and identified. ZEN coupled to bovine serum albumin (BSA) via 1,4-butanediol diglycidyl ether was prepared as immunogen. The mAb showed low cross-reactivity with five ZEN analogues. Using an antibody preparation with a titer of ≥1:5.12 × 10(5), the cross-reactivity (CR) of the anti-ZEN monoclonal antibody with four of the analogues was <4%, except for zearalanone, which was 53.121%. The recovery rates of ZEN in spiked corn samples were in the range of 91.30-97.07% with coefficients of variation <5.32%. An immunochromatographic strip was developed using the specific anti-ZEN monoclonal antibody and applied to the screening of corn samples for ZEN residues. The test could be accomplished within 5-10 min. The sensitivity of the test strip in corn sample extract was confirmed to be 20 μg/kg by unaided visual assessment, and the IC50 was calculated as 3.4 ng/mL using a test strip reader. The test strip, analyzed by unaided visual assessment and strip reader, showed very good agreement with competitive indirect ELISA and high-performance liquid chromatography (HPLC) analysis for naturally contaminated corn samples.
authors.affiliation: Key Laboratory of Animal Immunology of the Ministry of Agriculture, Henan Academy of Agricultural Sciences , Zhengzhou 450002, China.
authors.forename: Yaning
authors.initials: Y
authors.lastname: Sun
authors.forename: Xiaofei
authors.initials: X
authors.lastname: Hu
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhang
authors.forename: Jifei
authors.initials: J
authors.lastname: Yang
authors.forename: Fangyu
authors.initials: F
authors.lastname: Wang
authors.forename: Yao
authors.initials: Y
authors.lastname: Wang
authors.forename: Ruiguang
authors.initials: R
authors.lastname: Deng
authors.forename: Gaiping
authors.initials: G
authors.lastname: Zhang
mesh_terms: D000906:Antibodies / Q000032:analysis; D002846:Chromatography, Affinity / Q000295:instrumentation / Q000379:methods*; D005506:Food Contamination / Q000032:analysis*; D003313:Zea mays / Q000737:chemistry*; D015025:Zearalenone / Q000032:analysis* / Q000302:isolation & purification
chemical_list: D000906:Antibodies; D015025:Zearalenone
keywords: colloidal gold; immunoassay; monoclonal antibody; test strip; zearalenone