title: Map-based cloning of a candidate gene conferring Fusarium yellows resistance in Brassica oleracea.
abstract: We identified the candidate gene conferring yellow wilt resistance (YR) in B. oleracea . This work will facilitate YR breeding programs for B. oleracea and its closely related species. Yellow wilt disease is one of the most serious diseases of cabbage worldwide. Type A resistance to the disease is controlled by a single dominant gene that is used in cabbage breeding. Our previous QTL study identified the FocBo1 locus controlling type A resistance. In this study, the FocBo1 locus was fine-mapped by using 139 recombinant F2 plants derived from resistant cabbage (AnjuP01) and susceptible broccoli (GCP04) DH lines. As a result, we successfully delimited the location of FocBo1 within 1.00 cM between markers, BoInd 2 and BoInd 11. Analysis of BAC and cosmid sequences corresponding to the FocBo1 locus identified an orthologous gene of Bra012688 that was recently identified as an candidate gene that confers yellows resistance in Chinese cabbage. The candidate gene-specific DNA markers and phenotypes in F1 cabbage cultivars and their selfed F2 populations showed a perfect correlation. Our identification of the candidate gene for FocBo1 will assist introduction of fusarium resistance into B. oleracea cultivars and contribute further understanding of interaction between Brassica plants and fusarium.
authors.affiliation: Laboratory of Plant Breeding, Graduate School of Science and Technology, Faculty of Agriculture, Niigata University, 2-8050, Ikarashi, Nishi-ku, Niigata, 950-2181, Japan.
authors.forename: Motoki
authors.initials: M
authors.lastname: Shimizu
authors.forename: Zi-jing
authors.initials: ZJ
authors.lastname: Pu
authors.forename: Takahiro
authors.initials: T
authors.lastname: Kawanabe
authors.forename: Hiroyasu
authors.initials: H
authors.lastname: Kitashiba
authors.forename: Satoru
authors.initials: S
authors.lastname: Matsumoto
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Ebe
authors.forename: Monari
authors.initials: M
authors.lastname: Sano
authors.forename: Taketo
authors.initials: T
authors.lastname: Funaki
authors.forename: Eigo
authors.initials: E
authors.lastname: Fukai
authors.forename: Ryo
authors.initials: R
authors.lastname: Fujimoto
authors.forename: Keiichi
authors.initials: K
authors.lastname: Okazaki
mesh_terms: D001937:Brassica / Q000235:genetics* / Q000382:microbiology; D001947:Breeding; D002874:Chromosome Mapping; D032461:Chromosomes, Plant; D003001:Cloning, Molecular; D018744:DNA, Plant / Q000235:genetics; D060467:Disease Resistance / Q000235:genetics*; D005670:Fusarium; D005799:Genes, Dominant; D017343:Genes, Plant; D005819:Genetic Markers; D005838:Genotype; D010641:Phenotype; D010935:Plant Diseases / Q000235:genetics / Q000382:microbiology; D017422:Sequence Analysis, DNA; D026801:Synteny
chemical_list: D018744:DNA, Plant; D005819:Genetic Markers