title: Target antigens for perinuclear antineutrophil cytoplasmic antibodies in Iranian patients with ulcerative colitis.
abstract: BACKGROUND Patients with ulcerative colitis (UC) carry autoantibodies such as perinuclear antineutrophil cytoplasmic antibodies (p-ANCA).
  
  
  OBJECTIVE
  The aim of the present study was to evaluate the target antigens for p-ANCA in Iranian patients with UC. METHODS p-ANCA target antigens including elastase, lactoferrin, cathepsin G, myeloproxidase, lysozyme, and bactericidal permeability increasing protein (BPI) were determined in 113 patients with UC using enzyme-linked immunosorbent assay (ELISA). RESULTS 59.2% of the patients were positive for at least one antigen and p-ANCA directed against lactoferrin, elastase, lysozyme, cathepsin G, Bactericidal permeability increasing protein, and myeloproxidase in 31.5%, 25.9%, 8.3%, 7.4%, 5.6%, and 0% of the patients, respectively. CONCLUSION The highest prevalence of p-ANCA was observed against lactoferrin and elastase. Also, myeloproxidase was not an antigen for p-ANCA among our patients.
authors.affiliation: Endoscopic & Minimally Invasive Surgery Research Center, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
authors.forename: Ali
authors.initials: A
authors.lastname: Bahari
authors.affiliation: Department of Inflammatory Bowel Disease, Research Center of Gastroenterology and Liver Diseases, Tehran, Iran ; Department of Biomedical & Molecular Sciences, Queen's University, Canada.
authors.forename: Mahmoud
authors.initials: M
authors.lastname: Aarabi
authors.affiliation: Clinical Laboratory, Research Center for Endocrinology and Metabolic Diseases, Tehran, Iran.
authors.forename: Mehdi
authors.initials: M
authors.lastname: Hedayati
authors.affiliation: Clinical Pathology Laboratory, Mofid Children Hospital, Tehran, Iran.
authors.forename: Ali
authors.initials: A
authors.lastname: Jarollahi
authors.affiliation: Research Institute of Gastroenterology and Liver Diseases (RIGLD), Shahid Beheshti University, Tehran, Iran.
authors.forename: Farzad
authors.initials: F
authors.lastname: Firouzi
authors.affiliation: Department of Inflammatory Bowel Disease, Research Center of Gastroenterology and Liver Diseases, Tehran, Iran.
authors.forename: Rahim
authors.initials: R
authors.lastname: Aghazadeh
authors.affiliation: Department of Inflammatory Bowel Disease, Research Center of Gastroenterology and Liver Diseases, Tehran, Iran.
authors.forename: Mohammad Reza
authors.initials: MR
authors.lastname: Zali
authors.affiliation: Department of Gastroenterology, Mazandaran University of Medical Sciences, Sari, Iran.
authors.forename: Zohreh
authors.initials: Z
authors.lastname: Bari
authors.affiliation: Department of Clinical Biochemistry, School of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran ; Cellular and Molecular Research Center, Zahedan University of Medical Sciences, Zahedan, Iran.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Hashemi
authors.affiliation: Department of Internal Medicine, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
authors.forename: Abbas
authors.initials: A
authors.lastname: Esmaeilzadeh
authors.affiliation: Department of Internal Medicine, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
authors.forename: Ali
authors.initials: A
authors.lastname: Mokhtarifar
authors.affiliation: Department of Internal Medicine, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
authors.forename: Azita
authors.initials: A
authors.lastname: Ganji
keywords: Anti-neutrophil cytoplasmic antibody; Inflammatory bowel disease; Ttarget antigen; Ulcerative colitis