title: Synthesis of and specific antibody generation for glycopeptides with arginine N-GlcNAcylation.
abstract: As a unique and unappreciated protein posttranslational modification, arginine N-glycosylation was recently discovered to play an important role in the process that bacteria counteract host defenses. To provide chemical tools for further proteomic and biochemical studies on arginine N-glycosylation, we report the first general strategy for a rapid and cost-effective synthesis of glycopeptides carrying single or multiple arginine N-GlcNAcyl groups. These glycopeptides were successfully utilized to generate the first antibodies that can specifically recognize arginine N-GlcNAcylated peptides or proteins in a sequence-independent manner.
authors.affiliation: Department of Organic Chemistry, School of Pharmacy, Second Military Medical University, Shanghai 200433 (China); Tsinghua-Peking Center for Life Sciences, Department of Chemistry, Tsinghua University, Beijing 100084 (China).
authors.forename: Man
authors.initials: M
authors.lastname: Pan
authors.forename: Shan
authors.initials: S
authors.lastname: Li
authors.forename: Xiang
authors.initials: X
authors.lastname: Li
authors.forename: Feng
authors.initials: F
authors.lastname: Shao
authors.forename: Lei
authors.initials: L
authors.lastname: Liu
authors.forename: Hong-Gang
authors.initials: HG
authors.lastname: Hu
mesh_terms: D000906:Antibodies / Q000737:chemistry* / Q000378:metabolism; D001120:Arginine / Q000737:chemistry*; D029968:Escherichia coli Proteins / Q000378:metabolism; D005944:Glucosamine / Q000737:chemistry*; D006020:Glycopeptides / Q000737:chemistry*; D006031:Glycosylation; D012834:Silver / Q000737:chemistry; D060327:Solid-Phase Synthesis Techniques; D037521:Virulence Factors / Q000378:metabolism
chemical_list: D000906:Antibodies; D029968:Escherichia coli Proteins; D006020:Glycopeptides; C553814:NleB protein, E coli; D037521:Virulence Factors; D012834:Silver; D001120:Arginine; D005944:Glucosamine
keywords: N-GlcNAcylation; antibodies; arginine; glycopeptides; solid-phase peptide synthesis