title: A compact Airy beam light sheet microscope with a tilted cylindrical lens.
abstract: Light-sheet imaging is rapidly gaining importance for imaging intact biological specimens. Many of the latest innovations rely on the propagation-invariant Bessel or Airy beams to form an extended light sheet to provide high resolution across a large field of view. Shaping light to realize propagation-invariant beams often relies on complex programming of spatial light modulators or specialized, custom made, optical elements. Here we present a straightforward and low-cost modification to the traditional light-sheet setup, based on the open-access light-sheet microscope OpenSPIM, to achieve Airy light-sheet illumination. This brings wide field single-photon light-sheet imaging to a broader range of endusers. Fluorescent microspheres embedded in agarose and a zebrafish larva were imaged to demonstrate how such a microscope can have a minimal footprint and cost without compromising on imaging quality.
authors.affiliation: SUPA, School of Physics and Astronomy, University of St Andrews, North Haugh, St Andrews, KY16 9SS, UK.
authors.forename: Zhengyi
authors.initials: Z
authors.lastname: Yang
authors.affiliation: SUPA, School of Physics and Astronomy, University of St Andrews, North Haugh, St Andrews, KY16 9SS, UK.
authors.forename: Martynas
authors.initials: M
authors.lastname: Prokopas
authors.affiliation: SUPA, School of Physics and Astronomy, University of St Andrews, North Haugh, St Andrews, KY16 9SS, UK ; School of Biology, Medical and Biological Sciences Building, University of St Andrews, North Haugh, St Andrews, KY16 9TF, UK.
authors.forename: Jonathan
authors.initials: J
authors.lastname: Nylk
authors.affiliation: The Scottish Oceans Institute, Gatty Marine Laboratory, School of Biology, University of St Andrews, East Sands, St Andrews, KY16 8LB, UK.
authors.forename: Clara
authors.initials: C
authors.lastname: Coll-Lladó
authors.affiliation: School of Biology, Medical and Biological Sciences Building, University of St Andrews, North Haugh, St Andrews, KY16 9TF, UK.
authors.forename: Frank J
authors.initials: FJ
authors.lastname: Gunn-Moore
authors.affiliation: The Scottish Oceans Institute, Gatty Marine Laboratory, School of Biology, University of St Andrews, East Sands, St Andrews, KY16 8LB, UK.
authors.forename: David E K
authors.initials: DE
authors.lastname: Ferrier
authors.affiliation: SUPA, School of Physics and Astronomy, University of St Andrews, North Haugh, St Andrews, KY16 9SS, UK.
authors.forename: Tom
authors.initials: T
authors.lastname: Vettenburg
authors.affiliation: SUPA, School of Physics and Astronomy, University of St Andrews, North Haugh, St Andrews, KY16 9SS, UK.
authors.forename: Kishan
authors.initials: K
authors.lastname: Dholakia
keywords: (070.0070) Fourier optics and signal processing; (110.0180) Microscopy; (110.1758) Computational imaging; (110.4850) Optical transfer functions; (110.7348) Wavefront encoding; (140.3300) Laser beam shaping; (170.0170) Medical optics and biotechnology; (180.6900) Three-dimensional microscopy; (220.1000) Aberration compensation