title: Tumor targeting, trifunctional dendritic wedge.
abstract: We report in vitro and in vivo evaluation of a newly designed trifunctional theranostic agent for targeting solid tumors. This agent combines a dendritic wedge with high boron content for boron neutron capture therapy or boron MRI, a monomethine cyanine dye for visible-light fluorescent imaging, and an integrin ligand for efficient tumor targeting. We report photophysical properties of the new agent, its cellular uptake and in vitro targeting properties. Using live animal imaging and intravital microscopy (IVM) techniques, we observed a rapid accumulation of the agent and its retention for a prolonged period of time (up to 7 days) in fully established animal models of human melanoma and murine mammary adenocarcinoma. This macromolecular theranostic agent can be used for targeted delivery of high boron load into solid tumors for future applications in boron neutron capture therapy.
authors.affiliation: Department of Pharmacology and Pharmaceutical Sciences, University of Southern California , 1985 Zonal Avenue, PSC B15C, HSC 9121, Los Angeles, California 90089, United States.
authors.forename: Ramin
authors.initials: R
authors.lastname: Dubey
authors.forename: Swati
authors.initials: S
authors.lastname: Kushal
authors.forename: Alexis
authors.initials: A
authors.lastname: Mollard
authors.forename: Lesya
authors.initials: L
authors.lastname: Vojtovich
authors.forename: Philip
authors.initials: P
authors.lastname: Oh
authors.forename: Michael D
authors.initials: MD
authors.lastname: Levin
authors.forename: Jan E
authors.initials: JE
authors.lastname: Schnitzer
authors.forename: Ilya
authors.initials: I
authors.lastname: Zharov
authors.forename: Bogdan Z
authors.initials: BZ
authors.lastname: Olenyuk
mesh_terms: D000818:Animals; D001692:Biological Transport; D045744:Cell Line, Tumor; D002470:Cell Survival; D050091:Dendrimers / Q000378:metabolism / Q000493:pharmacokinetics / Q000627:therapeutic use*; D006801:Humans; D016023:Integrins / Q000378:metabolism; D008024:Ligands; D051379:Mice; D009369:Neoplasms / Q000175:diagnosis* / Q000473:pathology / Q000628:therapy*; D014018:Tissue Distribution
chemical_list: D050091:Dendrimers; D016023:Integrins; D008024:Ligands