title: Postsynaptic insertion of AMPA receptor onto cortical pyramidal neurons in the anterior cingulate cortex after peripheral nerve injury.
abstract: Long-term potentiation (LTP) is the key cellular mechanism for physiological learning and pathological chronic pain. Postsynaptic accumulation of AMPA receptor (AMPAR) GluA1 plays an important role for injury-related cortical LTP. However, there is no direct evidence for postsynaptic GluA1 insertion or accumulation after peripheral injury. Here we report nerve injury increased the postsynaptic expression of AMPAR GluA1 in pyramidal neurons in the layer V of the anterior cingulate cortex (ACC), including the corticospinal projecting neurons. Electrophysiological recordings show that potentiation of postsynaptic responses was reversed by Ca2+ permeable AMPAR antagonist NASPM. Finally, behavioral studies show that microinjection of NASPM into the ACC inhibited behavioral sensitization caused by nerve injury. Our findings provide direct evidence that peripheral nerve injury induces postsynaptic GluA1 accumulation in cingulate cortical neurons, and inhibits postsynaptic GluA1 accumulation which may serve as a novel target for treating neuropathic pain.
authors.forename: Tao
authors.initials: T
authors.lastname: Chen
authors.forename: Wen
authors.initials: W
authors.lastname: Wang
authors.forename: Yu-Lin
authors.initials: YL
authors.lastname: Dong
authors.forename: Ming-Ming
authors.initials: MM
authors.lastname: Zhang
authors.forename: Jian
authors.initials: J
authors.lastname: Wang
authors.forename: Kohei
authors.initials: K
authors.lastname: Koga
authors.forename: Yong-Hui
authors.initials: YH
authors.lastname: Liao
authors.forename: Jin-Lian
authors.initials: JL
authors.lastname: Li
authors.forename: Timotheus
authors.initials: T
authors.lastname: Budisantoso
authors.forename: Ryuichi
authors.initials: R
authors.lastname: Shigemoto
authors.forename: Makoto
authors.initials: M
authors.lastname: Itakura
authors.forename: Richard L
authors.initials: RL
authors.lastname: Huganir
authors.forename: Yun-Qing
authors.initials: YQ
authors.lastname: Li
authors.forename: Min
authors.initials: M
authors.lastname: Zhuo
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism; D017868:Cyclic AMP-Dependent Protein Kinases / Q000378:metabolism; D003712:Dendrites / Q000378:metabolism / Q000648:ultrastructure; D006179:Gyrus Cinguli / Q000473:pathology*; D006930:Hyperalgesia / Q000473:pathology; D017774:Long-Term Potentiation; D008297:Male; D008810:Mice, Inbred C57BL; D059348:Peripheral Nerve Injuries; D010766:Phosphorylation; D021122:Protein Subunits / Q000378:metabolism; D017966:Pyramidal Cells / Q000378:metabolism* / Q000473:pathology*; D018091:Receptors, AMPA / Q000378:metabolism*; D013116:Spinal Cord / Q000378:metabolism / Q000473:pathology; D013569:Synapses / Q000378:metabolism*
chemical_list: D002118:Calcium; D017868:Cyclic AMP-Dependent Protein Kinases; D021122:Protein Subunits; D018091:Receptors, AMPA