title: A real-time PCR approach based on SPF10 primers and the INNO-LiPA HPV genotyping extra assay for the detection and typing of human papillomavirus.
abstract: A highly sensitive SPF10 real-time PCR was developed to achieve simultaneous amplification and detection of the human papillomavirus (HPV) target. That way, LiPA analysis of the HPV-negative samples can be avoided, reducing workload and cost. Here, we describe in detail a SYBR Green I-based real-time PCR assay based on SPF10 primers using the LightCycler(®) 480 system to generate and detect HPV amplicons, which are compatible with the LiPA assay.
authors.affiliation: Applied Molecular Biology Research (AMBIOR) group, Laboratory of Cell Biology and Histology, Vaccine & Infectious Diseases Institute (VAXINFECTIO), University of Antwerp (Campus Groenenborger), Groenenborgerlaan 171, B-2020, Antwerp, Belgium, Isabel.Micalessi@ua.ac.be.
authors.forename: M Isabel
authors.initials: MI
authors.lastname: Micalessi
authors.forename: Gaëlle A
authors.initials: GA
authors.lastname: Boulet
authors.forename: Johannes
authors.initials: J
authors.lastname: Bogers
mesh_terms: D017931:DNA Primers / Q000378:metabolism*; D005838:Genotype; D060005:Genotyping Techniques / Q000379:methods*; D006801:Humans; D058889:Molecular Typing / Q000379:methods*; D009691:Nucleic Acid Denaturation / Q000235:genetics; D027383:Papillomaviridae / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*
chemical_list: D017931:DNA Primers