title: Synthesis and study of a molecularly imprinted polymer for specific solid-phase extraction of vinflunine and its metabolite from biological fluids.
abstract: A molecularly imprinted polymer (MIP) was synthesized in order to specifically extract vinflunine, an anticancer agent, and its metabolite (4-O-deacetylvinflunine) from bovine plasma and artificial urine by solid-phase extraction (SPE). Vinorelbine, a non-fluorinated analogue of vinflunine, was selected as a template for MIP synthesis. The selectivity of MIP versus the template (vinorelbine) and other alkaloids (catharanthine, vinblastine, vincristine, vinflunine and 4-O-deacetylvinflunine) was shown by a SPE protocol carried out with non-aqueous samples. A second protocol was developed for aqueous samples with two consecutive washing steps (AcOH-NH2 OH buffer (pH 7, I=10 mM)-MeOH mixture 95:5 v/v and ACN-AcOH mixture 99:1 v/v) and an elution step (MeOH-AcOH mixture 90:10 v/v). Thus, MIP-SPE of bovine plasma brought high recoveries, 81 and 89% for vinflunine and its metabolite, respectively. This protocol was slightly modified for artificial urine samples in order to obtain a good MIP/NIP selectivity; furthermore, elution recoveries were 73 and 81% for vinflunine and its metabolite, respectively. Repeatability was assessed in both biological matrices and RSD (%) were inferior to 4%. The MIP also showed a suitable linearity (r(2) superior to 0.99), between 0.25 and 10 μg/mL for plasma, and between 1 and 5 μg/mL for artificial urine.
authors.affiliation: Institut de Chimie Organique et Analytique, Université d'Orléans -CNRS FR 2708- UMR 6005, Orléans, France.
authors.forename: Claire
authors.initials: C
authors.lastname: Lopez
authors.forename: Bérengère
authors.initials: B
authors.lastname: Claude
authors.forename: Philippe
authors.initials: P
authors.lastname: Morin
authors.forename: Martine
authors.initials: M
authors.lastname: Pelissou
authors.forename: Richard
authors.initials: R
authors.lastname: Pena
authors.forename: Jean-Paul
authors.initials: JP
authors.lastname: Max
authors.forename: Jean-Paul
authors.initials: JP
authors.lastname: Ribet
mesh_terms: D001826:Body Fluids / Q000737:chemistry* / Q000378:metabolism; D054802:Molecular Imprinting; D015394:Molecular Structure; D011108:Polymers / Q000138:chemical synthesis* / Q000737:chemistry; D052616:Solid Phase Extraction / Q000379:methods*; D014747:Vinblastine / Q000031:analogs & derivatives* / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism
chemical_list: D011108:Polymers; C111217:vinflunine; D014747:Vinblastine
keywords: Biological fluids; Metabolite; Molecularly imprinted polymer; SPE; Vinflunine