title: [Impact of miR-148a on the proliferation of gastric carcinoma MKN45 cells and its mechanism].
abstract: OBJECTIVE
  To investigate the influence of miR-148a on cell proliferation of human gastric cancer cell lines MKN45.
  
  
  METHODS
  Expression level of miR-148a was detected by qRT-PCR in the carcinoma tissues and the tissues adjacent to carcinoma of 60 patients with gastric cancer. Lentivirus packaging was used to establish MKN45 gastric cancer cell line expressing stable miR-148a as transfection group,and the untransfected MKN45 cell line as the control group. Gastric cancer MKN45 cell proliferation was detected by CCK8 method. The target gene of miR-148a was predicted by targetscan. Expression of target gene was examined by Western blotting.
  
  
  RESULTS
  Expression of miR-148a in gastric cancer tissues of 54 cases(90.0%, 54/60) decreased, and among them, 37 tissue samples(61.7%, 37/60) decreased by 2 times. mir-148a expression in cancer tissues of patients with lymph node metastasis, vascular invasion and TNM stage III(-IIII( was down-regulated more obviously(P<0.05). From the third day after transfection, the growth of MKN45 cells was significantly inhibited(P<0.01). Gene CDC25B might be the target gene of miR-148a according to the results of targetscan. Western blot showed that CDC25B expression in transfection group was lower as compared to control group.
  
  
  CONCLUSIONS
  MiR-148a expression is down-regulated in gastric cancer tissues and inhibits gastric cancer cell proliferation. CDC25B may be the target gene of miR-148a that plays a role in tumor suppressor.
authors.affiliation: Department of General surgery, Shanghai First People's Hospital, Shanghai Jiaotong University, School of Medicine, Shanghai 200080, China. jkli65975@163.com.
authors.forename: Guangting
authors.initials: G
authors.lastname: Qiu
authors.forename: Suihan
authors.initials: S
authors.lastname: Wang
authors.forename: Dapeng
authors.initials: D
authors.lastname: Li
authors.forename: Jikun
authors.initials: J
authors.lastname: Li
mesh_terms: D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015536:Down-Regulation; D006801:Humans; D008207:Lymphatic Metastasis; D035683:MicroRNAs / Q000235:genetics*; D009367:Neoplasm Staging; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013274:Stomach Neoplasms / Q000235:genetics*; D014162:Transfection
chemical_list: D035683:MicroRNAs