title: The role of nitric oxide in the pathogenesis and severity of psoriasis.
abstract: The aim of our study was to investigate the possible role of nitrogen reactive species in pathogenesis of psoriasis. A total of 187 individuals were included in this study, out of these 84 were patients suffering from psoriasis and 103 were healthy subjects, served as a control. Patients with psoriasis were graded according to the Psoriasis Area Severity Index (PASI), presenting at the time of blood collection. After obtaining prior consent, about 2 ml of random blood was collected for estimation blood free nitric oxide (NO) content by Electron Paramagnetic Resonance (EPR) Spectroscopic method. For detection of NO in blood the spin-trap (diethilditiocarbamate (DETC) (Sigma) was used. In blood samples of patients with psoriasis nitrosilated hemoglobin (HbNO) complexes and alterations of free spin-trapped NO EPR signal intensity and were detected. Free NO content in blood decreased with the increasing severity of the psoriasis. It may be concluded that under oxidative stress conditions during psoriasis the decrease level of free nitric oxide in the patient's blood may contribute to a violation of the vasomotor activity of subcutaneous capillaries, impairment of skin blood supply, development of hypoxia, exacerbation of oxidative stress, alteration of immune balance, spreading of skin infection and exacerbation of the severity of psoriasis. Use of NO-containing creams will contribute to a partial recovery of disturbed functions and remission of the disease.
authors.affiliation: Tbilisi State Medical University, Department of Dermatology and Venereology, Department of Medical Physics and Biophysics, Department of Human Normal Anatomy, Georgia; University of Rome вЂњSapienzaвЂќ, Department of Dermatology and Venereology, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Matoshvili
authors.affiliation: Tbilisi State Medical University, Department of Dermatology and Venereology, Department of Medical Physics and Biophysics, Department of Human Normal Anatomy, Georgia; University of Rome вЂњSapienzaвЂќ, Department of Dermatology and Venereology, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Katsitadze
authors.affiliation: Tbilisi State Medical University, Department of Dermatology and Venereology, Department of Medical Physics and Biophysics, Department of Human Normal Anatomy, Georgia; University of Rome вЂњSapienzaвЂќ, Department of Dermatology and Venereology, Italy.
authors.forename: T
authors.initials: T
authors.lastname: Sanikidze
authors.affiliation: Tbilisi State Medical University, Department of Dermatology and Venereology, Department of Medical Physics and Biophysics, Department of Human Normal Anatomy, Georgia; University of Rome вЂњSapienzaвЂќ, Department of Dermatology and Venereology, Italy.
authors.forename: D
authors.initials: D
authors.lastname: Tophuria
authors.affiliation: Tbilisi State Medical University, Department of Dermatology and Venereology, Department of Medical Physics and Biophysics, Department of Human Normal Anatomy, Georgia; University of Rome вЂњSapienzaвЂќ, Department of Dermatology and Venereology, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Richetta
authors.affiliation: Tbilisi State Medical University, Department of Dermatology and Venereology, Department of Medical Physics and Biophysics, Department of Human Normal Anatomy, Georgia; University of Rome вЂњSapienzaвЂќ, Department of Dermatology and Venereology, Italy.
authors.forename: S
authors.initials: S
authors.lastname: D'Epiro
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D002648:Child; D004578:Electron Spin Resonance Spectroscopy; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009569:Nitric Oxide / Q000097:blood*; D018384:Oxidative Stress; D011565:Psoriasis / Q000097:blood* / Q000503:physiopathology
chemical_list: D009569:Nitric Oxide