title: Molecular detection of human papillomavirus in Brazilian women with cervical intraepithelial neoplasia in a northeast Brazilian city.
abstract: We examined the prevalence of human papillomavirus (HPV) infection in Brazilian women with cervical intraepithelial neoplasia. Our goal was to identify the types of HPV and their association with risk factors. This prospective cross-sectional study included 97 samples collected from women aged 14-79 years at the public health units of gynecological care in São Luís, MA, Brazil. HPV detection was performed by nested polymerase chain reaction and sequence analysis. The study patients completed a structured questionnaire to provide information regarding their socio-demographic, clinical, and behavioral status. HPV prevalence was found to be 80.4%, with 17 virus types detected, including HPV 16, 18, 58, 6, and 11. Significant associations between HPV infection and age and frequency of doctor visits were identified. The study findings indicate the significance of age and low frequency of visits to the gynecologist as risk factors for genital HPV infection, suggesting that HPV infection-derived cervical cancer could be prevented through orientation programs for women, which include sex education and information regarding screening tests. We also found an increased prevalence of high-risk HPV serotypes in cervical lesions, which reveals an association between cervical lesions and high-risk HPV.
authors.affiliation: Programa de Pós-Graduação em Saúde Materno-Infantil, Universidade Federal do Maranhão, São Luís, MA, Brasil jomdiogo@yahoo.com.br.
authors.forename: J D C
authors.initials: JD
authors.lastname: Nunes
authors.affiliation: Programa de Pós-Graduação em Saúde Materno-Infantil, Universidade Federal do Maranhão, São Luís, MA, Brasil.
authors.forename: F C B
authors.initials: FC
authors.lastname: Vidal
authors.affiliation: Programa de Pós-Graduação em Oncologia, Instituto Nacional de Câncer, Rio de Janeiro, RJ, Brasil.
authors.forename: C T L
authors.initials: CT
authors.lastname: Ferraro
authors.affiliation: Programa de Pós-Graduação em Saúde Materno-Infantil, Universidade Federal do Maranhão, São Luís, MA, Brasil.
authors.forename: M B C
authors.initials: MB
authors.lastname: Chein
authors.affiliation: Programa de Pós-Graduação em Saúde Materno-Infantil, Universidade Federal do Maranhão, São Luís, MA, Brasil.
authors.forename: L M O
authors.initials: LM
authors.lastname: Brito
authors.affiliation: Programa de Pós-Graduação em Saúde Materno-Infantil, Universidade Federal do Maranhão, São Luís, MA, Brasil.
authors.forename: S C M
authors.initials: SC
authors.lastname: Monteiro
mesh_terms: D000293:Adolescent; D000328:Adult; D000367:Age Factors; D000368:Aged; D001938:Brazil / Q000453:epidemiology; D003430:Cross-Sectional Studies; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics; D005260:Female; D054884:Host-Pathogen Interactions; D006801:Humans; D008875:Middle Aged; D027383:Papillomaviridae / Q000145:classification / Q000235:genetics / Q000502:physiology*; D030361:Papillomavirus Infections / Q000175:diagnosis / Q000453:epidemiology / Q000821:virology*; D016133:Polymerase Chain Reaction; D015995:Prevalence; D011446:Prospective Studies; D012307:Risk Factors; D017422:Sequence Analysis, DNA; D013045:Species Specificity; D011795:Surveys and Questionnaires; D002583:Uterine Cervical Neoplasms / Q000821:virology*; D055815:Young Adult; D002578:Uterine Cervical Dysplasia / Q000821:virology*
chemical_list: D004279:DNA, Viral