title: Activated P2X7 receptor upregulates the expression levels of NALP3 in P388D1 murine macrophage‑like cells.
abstract: The aims of the current study were to systematically analyze the regulation of the expression of NALP3 by the P2X7 receptor in P388D1 murine macrophage‑like cells, and to investigate the association between the P2X7 receptor and the NALP3 at the molecular level. Cell culture, RNA transfection, adenosine triphosphate (ATP)‑induced expression of NALP3, reverse transcription polymerase chain reaction and western blotting were used to explore the association between the P2X7 receptor and NALP3‑encoding gene in P388D1 murine macrophage‑like cells at the molecular level. The ATP‑activated P2X7 receptor can induce the upregulation of NALP3 expression at the gene and protein levels in P388D1 cells. These results demonstrated that the activation of P2X7 increases the expression levels of NALP3 in P388D1 murine macrophage‑like cells.
authors.affiliation: Department of Gastrointestinal Surgery, The Second Affiliated Hospital of Dalian Medical University, Dalian, Liaoning 116023, P.R. China.
authors.forename: Zhe
authors.initials: Z
authors.lastname: Chen
authors.affiliation: Department of Gastrointestinal Surgery, The Second Affiliated Hospital of Dalian Medical University, Dalian, Liaoning 116023, P.R. China.
authors.forename: Hongfei
authors.initials: H
authors.lastname: Jin
authors.affiliation: Department of Clinical Biochemistry, Dalian Medical University, Dalian, Liaoning 116044, P.R. China.
authors.forename: Yahui
authors.initials: Y
authors.lastname: Hou
authors.affiliation: Department of Gastrointestinal Surgery, The Second Affiliated Hospital of Dalian Medical University, Dalian, Liaoning 116023, P.R. China.
authors.forename: Bo
authors.initials: B
authors.lastname: Wang
authors.affiliation: Department of Gastrointestinal Surgery, The Second Affiliated Hospital of Dalian Medical University, Dalian, Liaoning 116023, P.R. China.
authors.forename: Chunlin
authors.initials: C
authors.lastname: Chen
authors.affiliation: Department of Clinical Biochemistry, Dalian Medical University, Dalian, Liaoning 116044, P.R. China.
authors.forename: Ziyi
authors.initials: Z
authors.lastname: Yang
authors.affiliation: Department of Gastrointestinal Surgery, The Second Affiliated Hospital of Dalian Medical University, Dalian, Liaoning 116023, P.R. China.
authors.forename: Dongbing
authors.initials: D
authors.lastname: Ding
authors.affiliation: Department of Clinical Biochemistry, Dalian Medical University, Dalian, Liaoning 116044, P.R. China.
authors.forename: Yunfei
authors.initials: Y
authors.lastname: Zuo
authors.affiliation: Department of Gastrointestinal Surgery, The Second Affiliated Hospital of Dalian Medical University, Dalian, Liaoning 116023, P.R. China.
authors.forename: Shuangyi
authors.initials: S
authors.lastname: Ren
mesh_terms: D000255:Adenosine Triphosphate / Q000494:pharmacology; D000818:Animals; D015153:Blotting, Western; D002352:Carrier Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D008264:Macrophages / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D000071199:NLR Family, Pyrin Domain-Containing 3 Protein; D034622:RNA Interference; D012333:RNA, Messenger / Q000378:metabolism; D034741:RNA, Small Interfering / Q000378:metabolism; D058486:Receptors, Purinergic P2X7 / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D015854:Up-Regulation / Q000187:drug effects
chemical_list: D002352:Carrier Proteins; D000071199:NLR Family, Pyrin Domain-Containing 3 Protein; C492716:Nlrp3 protein, mouse; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D058486:Receptors, Purinergic P2X7; D000255:Adenosine Triphosphate