title: Characterization of CaHsp70-1, a pepper heat-shock protein gene in response to heat stress and some regulation exogenous substances in Capsicum annuum L.
abstract: Pepper (Capsicum annuum L.) is sensitive to heat stress (HS). Heat shock proteins 70 (Hsp70s) play a crucial role in protecting plant cells against HS and control varies characters in different plants. However, CaHsp70-1 gene was not well characterized in pepper. In this study, CaHsp70-1 was cloned from the pepper thermotolerant line R9, which encoded a protein of 652 amino acids, with a molecular weight of 71.54 kDa and an isoelectric point of 5.20. CaHsp70-1 belongs to the cytosolic Hsp70 subgroup, and best matched with tomato SlHsp70. CaHsp70-1 was highly induced in root, stem, leaf and flower in R9 with HS treatment (40 °C for 2 h). In both thermosensitive line B6 and thermotolerant line R9, CaHsp70-1 significantly increased after 0.5 h of HS (40 °C), and maintained in a higher level after 4 h HS. The expression of CaHsp70-1 induced by CaCl2, H2O2 and putrescine (Put) under HS were difference between B6 and R9 lines. The different expression patterns may be related to the differences in promoters of CaHsp70-1 from the two lines. These results suggest that CaHsp70-1 as a member of cytosolic Hsp70 subgroup, may be involved in HS defense response via a signal transduction pathway contained Ca2+, H2O2 and Put.
authors.affiliation: College of Horticulture, Northwest A&F University, Yangling 712100, China. guomeng19881025@126.com.
authors.forename: Meng
authors.initials: M
authors.lastname: Guo
authors.affiliation: College of Horticulture, Northwest A&F University, Yangling 712100, China. 18829785690@163.com.
authors.forename: Yu-Fei
authors.initials: YF
authors.lastname: Zhai
authors.affiliation: College of Horticulture, Northwest A&F University, Yangling 712100, China. lujinpingok@163.com.
authors.forename: Jin-Ping
authors.initials: JP
authors.lastname: Lu
authors.affiliation: College of Horticulture, Northwest A&F University, Yangling 712100, China. 18717360273@163.com.
authors.forename: Lin
authors.initials: L
authors.lastname: Chai
authors.affiliation: Institute of Vegetables, Hangzhou Academy of Agricultural Sciences, Hangzhou 310024, China. kuni@21cn.com.
authors.forename: Wei-Guo
authors.initials: WG
authors.lastname: Chai
authors.affiliation: College of Horticulture, Northwest A&F University, Yangling 712100, China. zhgong@nwsuaf.edu.cn.
authors.forename: Zhen-Hui
authors.initials: ZH
authors.lastname: Gong
authors.affiliation: College of Horticulture, Northwest A&F University, Yangling 712100, China. xnjacklu@nwsuaf.edu.cn.
authors.forename: Ming-Hui
authors.initials: MH
authors.lastname: Lu
mesh_terms: D001483:Base Sequence; D002122:Calcium Chloride / Q000494:pharmacology; D002212:Capsicum / Q000378:metabolism*; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism*; D006861:Hydrogen Peroxide / Q000633:toxicity; D008969:Molecular Sequence Data; D008970:Molecular Weight; D059828:Plant Cells / Q000378:metabolism; D018515:Plant Leaves / Q000378:metabolism; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D011700:Putrescine / Q000494:pharmacology; D013696:Temperature
chemical_list: D018840:HSP70 Heat-Shock Proteins; D010940:Plant Proteins; D006861:Hydrogen Peroxide; D002122:Calcium Chloride; D011700:Putrescine