title: Optimization of growth inducing factors for colony forming and attachment of bone marrow-derived mesenchymal stem cells regarding bioengineering application.
abstract: OBJECTIVE
  These days, mesenchymal stem cells (MSCs) have received worldwide attention because of their potentiality in tissue engineering for implant dentistry. The purpose of this study was to evaluate various growth inducing factors in media for improvement of acquisition of bone marrow mesenchymal stem cells (BMMSCs) and colony forming unit-fibroblast (CFU-F).
  
  
  METHODS
  The mouse BMMSCs were freshly obtained from female C3H mouse femur and tibia. The cells seeded at the density of 10(6)/dish in media supplemented with different density of fetal bovine serum (FBS), 1α, 25-dihydroxyvitamin (VD3) and recombinant human epidermal growth factor (rhEGF). After 14 days, CFU-F assay was conducted to analyze the cell attachment and proliferation, and moreover for VD3, the 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay was additionally conducted.
  
  
  RESULTS
  The cell proliferation was increased with the increase of FBS concentration (P<.05). The cell proliferation was highest at the density of 20 ng/mL rhEGF compared with 0 ng/mL and 200 ng/mL rhEGF (P<.05). For VD3, although the colony number was increased with the increase of its concentration, the difference was not statistically significant (P>.05).
  
  
  CONCLUSIONS
  FBS played the main role in cell attachment and growth, and the growth factor like rhEGF played the additional effect. However, VD3 did not have much efficacy compare with the other two factors. Improvement of the conditions could be adopted to acquire more functional MSCs to apply into bony defect around implants easily.
authors.affiliation: Department of Prosthodontics & Dental Research Institute, Seoul National University Dental Hospital, School of Dentistry, Seoul National University, Seoul, Republic of Korea.
authors.forename: Hongxuan
authors.initials: H
authors.lastname: Quan
authors.affiliation: Department of Prosthodontics & Dental Research Institute, Seoul National University Dental Hospital, School of Dentistry, Seoul National University, Seoul, Republic of Korea.
authors.forename: Seong-Kyun
authors.initials: SK
authors.lastname: Kim
authors.affiliation: Department of Prosthodontics & Dental Research Institute, Seoul National University Dental Hospital, School of Dentistry, Seoul National University, Seoul, Republic of Korea.
authors.forename: Seong-Joo
authors.initials: SJ
authors.lastname: Heo
authors.affiliation: Department of Prosthodontics & Dental Research Institute, Seoul National University Dental Hospital, School of Dentistry, Seoul National University, Seoul, Republic of Korea.
authors.forename: Jai-Young
authors.initials: JY
authors.lastname: Koak
authors.affiliation: Department of Prosthodontics, Asan Medical Center, College of Medicine, University of Ulsan, Seoul, Republic of Korea.
authors.forename: Joo-Hee
authors.initials: JH
authors.lastname: Lee
keywords: 1α; 25-dihydroxyvitamin; Bone marrow mesenchymal stem cells; Cell proliferation; Fetal bovine serum; Recombinant human epidermal growth factor