title: Analysis of mechanisms of interaction between ciprofloxacin-based radiopharmaceutical labeled with technetium-99m and cultured Staphylococcus aureus.
abstract: Mechanisms of interaction between ciprofloxacin-based radiopharmaceutical labeled with technetium-99m and cultured Staphylococcus aureus were studied. The substance, reagent for radiopharmaceutical, and the radiopharmaceutical demonstrated similar bactericidal activity against the test bacterial cultures, which confirmed specific antibacterial activity of technetium-99m-labeled ciprofloxacin. The technetium-99m-labeled antibiotic reversibly interacts with inactivated Staphylococcus aureus culture, which indicates the existence of an additional non-specific pathway for radiopharmaceutical accumulation in the bacterial inflammation focus. Our experiment ruled out the possibility of binding free technetium-99m at concentrations <5% (its concentration in the radiopharmaceutical) with Staphylococcus aureus.
authors.affiliation: Laboratory of Radionuclide Analysis, Research Institute of Cardiology, Siberian Division of the Russian Academy of Medical Sciences, Tomsk, Russia, sazonova_si@mail.ru.
authors.forename: S I
authors.initials: SI
authors.lastname: Sazonova
authors.forename: M R
authors.initials: MR
authors.lastname: Karpova
authors.forename: Yu N
authors.initials: YN
authors.lastname: Il'yushenkova
authors.forename: N V
authors.initials: NV
authors.lastname: Varlamova
authors.forename: Yu B
authors.initials: YB
authors.lastname: Lishmanov
mesh_terms: D000900:Anti-Bacterial Agents / Q000378:metabolism / Q000494:pharmacology*; D002939:Ciprofloxacin / Q000378:metabolism / Q000494:pharmacology*; D052978:Disk Diffusion Antimicrobial Tests; D024881:Drug Resistance, Bacterial / Q000235:genetics / Q000502:physiology; D015609:Organotechnetium Compounds / Q000378:metabolism / Q000494:pharmacology*; D019275:Radiopharmaceuticals / Q000378:metabolism / Q000494:pharmacology*; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D015609:Organotechnetium Compounds; D019275:Radiopharmaceuticals; D002939:Ciprofloxacin