title: First evaluation of an outbreak of bovine babesiosis and anaplasmosis in Southern Brazil using multiplex PCR.
abstract: Outbreaks of tick-borne disease cases in Santa Catarina, Brazil are known, but the presence of the pathogen DNA has never been determined. In this study, the first survey of Anaplasma marginale, Babesia bigemina, and Babesia bovis DNA on blood samples of 33 cattle from an outbreak in Ponte Alta Municipality, Santa Catarina, Brazil, has been carried out. A multiplex PCR detected 54.5% of animals were co-infected with 2 or 3 parasites, while 24.2% were infected with only 1 species. The most prevalent agent was B. bigemina (63.6%) followed by A. marginale (60.6%). This is the first report of tick-borne disease pathogens obtained by DNA analysis in Southern Brazil.
authors.affiliation: Laboratório de Bioquímica de Hemoparasitas e Vetores, Centro de Ciências Agroveterinárias, Universidade do Estado de Santa Catarina, Lages, Brazil.
authors.forename: Mariana Feltrin
authors.initials: MF
authors.lastname: Canever
authors.affiliation: Laboratório de Bioquímica de Hemoparasitas e Vetores, Centro de Ciências Agroveterinárias, Universidade do Estado de Santa Catarina, Lages, Brazil.
authors.forename: Luisa Lemos
authors.initials: LL
authors.lastname: Vieira
authors.affiliation: Laboratório de Bioquímica de Hemoparasitas e Vetores, Centro de Ciências Agroveterinárias, Universidade do Estado de Santa Catarina, Lages, Brazil.
authors.forename: Carolina
authors.initials: C
authors.lastname: Reck
authors.affiliation: Companhia Integrada de Desenvolvimento Agrícola de Santa Catarina, Brazil.
authors.forename: Luisa
authors.initials: L
authors.lastname: Richter
authors.affiliation: Laboratório de Bioquímica de Hemoparasitas e Vetores, Centro de Ciências Agroveterinárias, Universidade do Estado de Santa Catarina, Lages, Brazil.
authors.forename: Luiz Claudio
authors.initials: LC
authors.lastname: Miletti
mesh_terms: D042322:Anaplasma marginale / Q000235:genetics / Q000302:isolation & purification; D000712:Anaplasmosis / Q000453:epidemiology*; D000818:Animals; D001403:Babesia / Q000235:genetics / Q000302:isolation & purification; D001404:Babesiosis / Q000453:epidemiology*; D001938:Brazil / Q000453:epidemiology; D002417:Cattle; D016054:DNA, Protozoan / Q000097:blood / Q000302:isolation & purification; D004196:Disease Outbreaks / Q000662:veterinary*; D016133:Polymerase Chain Reaction / Q000662:veterinary*
chemical_list: D016054:DNA, Protozoan
keywords: Anaplasma marginale; Babesia bigemina; Babesia bovis; Brazil; multiplex-PCR