title: Alcoholic extraction enables EPR analysis to characterize radiation-induced cellulosic signals in spices.
abstract: Different spices such as turmeric, oregano, and cinnamon were γ-irradiated at 1 and 10 kGy. The electron paramagnetic resonance (EPR) spectra of the nonirradiated samples were characterized by a single central signal (g = 2.006), the intensity of which was significantly enhanced upon irradiation. The EPR spectra of the irradiated spice samples were characterized by an additional triplet signal at g = 2.006 with a hyperfine coupling constant of 3 mT, associated with the cellulose radical. EPR analysis on various sample pretreatments in the irradiated spice samples demonstrated that the spectral features of the cellulose radical varied on the basis of the pretreatment protocol. Alcoholic extraction pretreatment produced considerable improvements of the EPR signals of the irradiated spice samples relative to the conventional oven and freeze-drying techniques. The alcoholic extraction process is therefore proposed as the most suitable sample pretreatment for unambiguous detection of irradiated spices by EPR spectroscopy.
authors.affiliation: School of Food Science & Biotechnology, Kyungpook National University , Daegu 702-701, Republic of Korea.
authors.forename: Jae-Jun
authors.initials: JJ
authors.lastname: Ahn
authors.forename: Bhaskar
authors.initials: B
authors.lastname: Sanyal
authors.forename: Kashif
authors.initials: K
authors.lastname: Akram
authors.forename: Joong-Ho
authors.initials: JH
authors.lastname: Kwon
mesh_terms: D002482:Cellulose / Q000737:chemistry*; D002935:Cinnamomum zeylanicum / Q000737:chemistry* / Q000528:radiation effects; D030024:Curcuma / Q000737:chemistry* / Q000528:radiation effects; D004578:Electron Spin Resonance Spectroscopy; D005514:Food Irradiation; D005720:Gamma Rays; D031341:Origanum / Q000737:chemistry* / Q000528:radiation effects; D017365:Spices / Q000032:analysis* / Q000528:radiation effects
chemical_list: D002482:Cellulose
keywords: EPR spectroscopy; detection of irradiated food; food irradiation; spices