title: Antimycobacterial Efficacy of Andrographis paniculata Leaf Extracts Under Intracellular and Hypoxic Conditions.
abstract: The inhibition of the growth of Mycobacterium tuberculosis by the extracts of Andrographis paniculata has been studied using intracellular and axenic hypoxic conditions. The inhibition (confirmed using the gold standard colony forming unit assay) was found to increase with "double stimuli" or higher concentration of the extract. Organic solvent extracts were found to inhibit bacterial growth more than the aqueous extracts under microaerophilic conditions mimicked through axenic and intracellular assays. This could be further explored to evaluate the potential of the plant to be used against nonreplicating/dormant bacilli.
authors.affiliation: The Foundation for Medical Research, Mumbai, India.
authors.forename: Purva
authors.initials: P
authors.lastname: Bhatter
authors.affiliation: The Foundation for Medical Research, Mumbai, India.
authors.forename: Pooja
authors.initials: P
authors.lastname: Gupta
authors.affiliation: The Foundation for Medical Research, Mumbai, India.
authors.forename: Poonam
authors.initials: P
authors.lastname: Daswani
authors.affiliation: Naoroji Godrej Centre for Plant Research, Satara, India.
authors.forename: Pundarikakshudu
authors.initials: P
authors.lastname: Tetali
authors.affiliation: The Foundation for Medical Research, Mumbai, India fmr@fmrindia.org.
authors.forename: Tannaz
authors.initials: T
authors.lastname: Birdi
mesh_terms: D035685:Andrographis / Q000737:chemistry*; D000995:Antitubercular Agents / Q000737:chemistry / Q000494:pharmacology*; D045744:Cell Line, Tumor; D004352:Drug Resistance, Microbial; D006801:Humans; D042541:Intracellular Space / Q000378:metabolism / Q000382:microbiology; D009169:Mycobacterium tuberculosis / Q000187:drug effects* / Q000378:metabolism; D010100:Oxygen / Q000378:metabolism; D010936:Plant Extracts / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry
chemical_list: D000995:Antitubercular Agents; D010936:Plant Extracts; D010100:Oxygen
keywords: Andrographis paniculata; Mycobacterium tuberculosis; axenic anaerobic assays; drug resistant isolates; intracellular assays