title: An insight into silver nanoparticles bioavailability in rats.
abstract: A comprehensive study of the bioavailability of orally administered silver nanoparticles (AgNPs) was carried out using a rat model. The silver uptake was monitored in liver and kidney tissues, as well as in urine and in feces. Significant accumulation of silver was found in both organs, the liver being the principal target of AgNPs. A significant (∼50%) fraction of silver was found in feces whereas the fraction excreted via urine was negligible (< 0.01%). Intact silver nanoparticles were found in feces by asymmetric flow field-flow fractionation (AsFlFFF) coupled with UV-Vis analysis. Laser ablation-ICP MS imaging showed that AgNPs were able to penetrate into the liver, in contrast to kidneys where they were retained in the cortex. Silver speciation analysis in cytosols from kidneys showed the metallothionein complex as the major species whereas in the liver the majority of silver was bound to high-molecular (70-25 kDa) proteins. These findings demonstrate the presence of Ag(i), released by the oxidation of AgNPs in the biological environment.
authors.affiliation: Group of Analytical Spectroscopy and Sensors (GEAS), Institute of Environmental Sciences (IUCA), University of Zaragoza, Pedro Cerbuna 12, 50009 Zaragoza, Spain. jjlamana@unizar.es.
authors.forename: Javier
authors.initials: J
authors.lastname: Jiménez-Lamana
authors.forename: Francisco
authors.initials: F
authors.lastname: Laborda
authors.forename: Eduardo
authors.initials: E
authors.lastname: Bolea
authors.forename: Isabel
authors.initials: I
authors.lastname: Abad-Álvaro
authors.forename: Juan R
authors.initials: JR
authors.lastname: Castillo
authors.forename: Juliusz
authors.initials: J
authors.lastname: Bianga
authors.forename: Man
authors.initials: M
authors.lastname: He
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Bierla
authors.forename: Sandra
authors.initials: S
authors.lastname: Mounicou
authors.forename: Laurent
authors.initials: L
authors.lastname: Ouerdane
authors.forename: Sylvie
authors.initials: S
authors.lastname: Gaillet
authors.forename: Jean-Max
authors.initials: JM
authors.lastname: Rouanet
authors.forename: Joanna
authors.initials: J
authors.lastname: Szpunar
mesh_terms: D000284:Administration, Oral; D000818:Animals; D001682:Biological Availability; D005243:Feces / Q000737:chemistry; D007668:Kidney / Q000737:chemistry; D008099:Liver / Q000737:chemistry; D008297:Male; D053768:Metal Nanoparticles / Q000008:administration & dosage / Q000032:analysis*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012834:Silver / Q000008:administration & dosage / Q000032:analysis* / Q000493:pharmacokinetics*
chemical_list: D012834:Silver