title: Design of covalently functionalized carbon nanotubes filled with metal oxide nanoparticles for imaging, therapy, and magnetic manipulation.
abstract: Nanocomposites combining multiple functionalities in one single nano-object hold great promise for biomedical applications. In this work, carbon nanotubes (CNTs) were filled with ferrite nanoparticles (NPs) to develop the magnetic manipulation of the nanotubes and their theranostic applications. The challenges were both the filling of CNTs with a high amount of magnetic NPs and their functionalization to form biocompatible water suspensions. We propose here a filling process using CNTs as nanoreactors for high-yield in situ growth of ferrite NPs into the inner carbon cavity. At first, NPs were formed inside the nanotubes by thermal decomposition of an iron stearate precursor. A second filling step was then performed with iron or cobalt stearate precursors to enhance the encapsulation yield and block the formed NPs inside the tubes. Water suspensions were then obtained by addition of amino groups via the covalent functionalization of the external surface of the nanotubes. Microstructural and magnetic characterizations confirmed the confinement of NPs into the anisotropic structure of CNTs making them suitable for magnetic manipulations and MRI detection. Interactions of highly water-dispersible CNTs with tumor cells could be modulated by magnetic fields without toxicity, allowing control of their orientation within the cell and inducing submicron magnetic stirring. The magnetic properties were also used to quantify CNTs cellular uptake by measuring the cell magnetophoretic mobility. Finally, the photothermal ablation of tumor cells could be enhanced by magnetic stimulus, harnessing the hybrid properties of NP loaded-CNTs.
authors.affiliation: Institut de Chimie et Procédés pour l'Energie, l'Environnement et la Santé (ICPEES), UMR-7515 CNRS-Université de Strasbourg , 25 rue Becquerel, 67087 Strasbourg Cedex 2, France.
authors.forename: Xiaojie
authors.initials: X
authors.lastname: Liu
authors.forename: Iris
authors.initials: I
authors.lastname: Marangon
authors.forename: Georgian
authors.initials: G
authors.lastname: Melinte
authors.forename: Claire
authors.initials: C
authors.lastname: Wilhelm
authors.forename: Cécilia
authors.initials: C
authors.lastname: Ménard-Moyon
authors.forename: Benoit P
authors.initials: BP
authors.lastname: Pichon
authors.forename: Ovidiu
authors.initials: O
authors.lastname: Ersen
authors.forename: Kelly
authors.initials: K
authors.lastname: Aubertin
authors.forename: Walid
authors.initials: W
authors.lastname: Baaziz
authors.forename: Cuong
authors.initials: C
authors.lastname: Pham-Huu
authors.forename: Sylvie
authors.initials: S
authors.lastname: Bégin-Colin
authors.forename: Alberto
authors.initials: A
authors.lastname: Bianco
authors.forename: Florence
authors.initials: F
authors.lastname: Gazeau
authors.forename: Dominique
authors.initials: D
authors.lastname: Bégin
mesh_terms: D003952:Diagnostic Imaging / Q000295:instrumentation*; D008280:Magnetics; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000627:therapeutic use; D046529:Microscopy, Electron, Transmission; D037742:Nanotubes, Carbon / Q000737:chemistry*; D010087:Oxides / Q000737:chemistry
chemical_list: D037742:Nanotubes, Carbon; D010087:Oxides
keywords: MRI; cell interactions; cobalt oxide nanoparticles; filling; functionalization; hyperthermia; iron oxide nanoparticles; magnetic carbon nanotubes; magnetic core−shell nanoparticles