title: Molecular characterization of an Apolipophorin-III gene from the Chinese oak silkworm, Antheraea pernyi (Lepidoptera: Saturniidae).
abstract: Apolipophorin-III (ApoLp-III) acts in lipid transport, lipoprotein metabolism, and innate immunity in insects. In this study, an ApoLp-III gene of Antheraea pernyi pupae (Ap-ApoLp-III) was isolated and characterized. The full-length cDNA of Ap-ApoLp-III is 687 bp, including a 5'-untranslated region (UTR) of 40 bp, 3'-UTR of 86 bp and an open reading frame of 561 bp encoding a polypeptide of 186 amino acids that contains an Apolipophorin-III precursor domain (PF07464). The deduced Ap-apoLp-III protein sequence has 68, 59, and 23% identity with its orthologs of Manduca sexta, Bombyx mori, and Aedes aegypti, respectively. Phylogenetic analysis showed that the Ap-apoLp-III was close to that of Bombycoidea. qPCR analysis revealed that Ap-ApoLp-III expressed during the four developmental stages and in integument, fat body, and ovaries. After six types of microorganism infections, expression levels of the Ap-ApoLp-III gene were upregulated significantly at different time points compared with control. RNA interference (RNAi) of Ap-ApoLp-III showed that the expression of Ap-ApoLp-III was significantly downregulated using qPCR after injection of E. coli. We infer that the Ap-ApoLp-III gene acts in the innate immunity of A. pernyi.
authors.affiliation: College of Life Sciences, Anhui Agricultural University, Hefei, P. R.China.
authors.forename: Qiu-Ning
authors.initials: QN
authors.lastname: Liu
authors.forename: Kun-Zhang
authors.initials: KZ
authors.lastname: Lin
authors.forename: Lin-Nan
authors.initials: LN
authors.lastname: Yang
authors.forename: Li-Shang
authors.initials: LS
authors.lastname: Dai
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.forename: Yu
authors.initials: Y
authors.lastname: Sun
authors.forename: Cen
authors.initials: C
authors.lastname: Qian
authors.forename: Guo-Qing
authors.initials: GQ
authors.lastname: Wei
authors.forename: Dong-Ran
authors.initials: DR
authors.lastname: Liu
authors.forename: Bao-Jian
authors.initials: BJ
authors.lastname: Zhu
authors.forename: Chao-Liang
authors.initials: CL
authors.lastname: Liu
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001053:Apolipoproteins / Q000096:biosynthesis / Q000235:genetics* / Q000276:immunology; D001483:Base Sequence; D018076:DNA, Complementary; D005260:Female; D007113:Immunity, Innate; D008018:Life Cycle Stages; D008297:Male; D008969:Molecular Sequence Data; D009036:Moths / Q000235:genetics* / Q000276:immunology / Q000382:microbiology; D016366:Open Reading Frames; D010802:Phylogeny; D034622:RNA Interference; D060888:Real-Time Polymerase Chain Reaction; D020506:Untranslated Regions
chemical_list: D001053:Apolipoproteins; D018076:DNA, Complementary; D020506:Untranslated Regions; C042470:apolipophorin III
keywords: Antheraea pernyi; apolipophorin III; expression; immune response