title: MBP-positive and CD11c-positive cells are associated with different phenotypes of Korean patients with non-asthmatic chronic rhinosinusitis.
abstract: BACKGROUND
  Asthmatic nasal polyps primarily exhibit eosinophilic infiltration. However, the identities of the immune cells that infiltrate non-asthmatic nasal polyps remain unclear. Thus, we thought to investigate the distribution of innate immune cells and its clinical relevance in non-asthmatic chronic rhinosinusitis (CRS) in Korea.
  
  
  METHODS
  Tissues from uncinate process (UP) were obtained from controls (n = 18) and CRS without nasal polyps (CRSsNP, n = 45). Nasal polyps (NP) and UP were obtained from CRS with nasal polyps (CRSwNP, n = 56). The innate immune cells was evaluated by immunohistochemistry such as, eosinophil major basic protein (MBP), tryptase, CD68, CD163, CD11c, 2D7, human neutrophil elastase (HNE) and its distribution was analyzed according to clinical parameters.
  
  
  RESULTS
  In comparisons between UP from each group, CRSwNP had a higher number of MPB(+), CD68(+), and CD11c(+) cells relative to CRSsNP. Comparisons between UP and NP from CRSwNP indicated that NP have a higher infiltrate of MBP(+), CD163(+), CD11c(+), 2D7(+) and HNE(+) cells, whereas fewer CD68(+) cells were found in NP. In addition, MBP(+) and CD11c(+) cells were increased from UP of CRSsNP, to UP of CRSwNP, and to NP of CRSwNP. Moreover, in UP from CRSwNP, the number of MBP(+) and CD11c(+) cells positively correlated with CT scores. In the analysis of CRSwNP phenotype, allergic eosinophilic polyps had a higher number of MBP(+), tryptase(+), CD11c(+), 2D7(+) cells than others, whereas allergic non-eosinophilic polyps showed mainly infiltration of HNE(+) and 2D7(+) cells.
  
  
  CONCLUSIONS
  The infiltration of MBP(+) and CD11c(+) innate immune cells show a significant association with phenotype and disease extent of CRS and allergic status also may influences cellular phenotype in non-asthmatic CRSwNP in Korea.
authors.affiliation: Department of Otorhinolaryngology-Head and Neck Surgery, Chuncheon Sacred Heart Hospital, Hallym University College of Medicine, Chuncheon, Korea.
authors.forename: Dong-Kyu
authors.initials: DK
authors.lastname: Kim
authors.affiliation: Department of Otorhinolaryngology-Head and Neck Surgery, Boramae Medical Center, Seoul National University College of Medicine, Seoul, Korea.
authors.forename: Min-Hyun
authors.initials: MH
authors.lastname: Park
authors.affiliation: Laboratory of Immunology and Infectious Diseases, Graduate School of Medical Science and Engineering, Korea Advanced Institute of Science and Technology, Daejeon, Korea.
authors.forename: Dong-Yeop
authors.initials: DY
authors.lastname: Chang
authors.affiliation: Department of Otorhinolaryngology-Head and Neck Surgery, Boramae Medical Center, Seoul National University College of Medicine, Seoul, Korea.
authors.forename: Kyung Mi
authors.initials: KM
authors.lastname: Eun
authors.affiliation: Department of Pharmacology and Biomedical Science, Ischemic/Hypoxic Disease Institute, Seoul National University College of Medicine, Seoul, Korea.
authors.forename: Hyun-Woo
authors.initials: HW
authors.lastname: Shin
authors.affiliation: Department of Otorhinolaryngology, Dankook University College of Medicine, Chonan, Korea.
authors.forename: Ji-Hun
authors.initials: JH
authors.lastname: Mo
authors.affiliation: Laboratory of Immunology and Infectious Diseases, Graduate School of Medical Science and Engineering, Korea Advanced Institute of Science and Technology, Daejeon, Korea.
authors.forename: Eui-Cheol
authors.initials: EC
authors.lastname: Shin
authors.affiliation: Department of Otorhinolaryngology-Head and Neck Surgery, Boramae Medical Center, Seoul National University College of Medicine, Seoul, Korea.
authors.forename: Hong Ryul
authors.initials: HR
authors.lastname: Jin
authors.affiliation: Department of Laboratory Medicine, Boramae Medical Center, Seoul National University College of Medicine, Seoul, Korea.
authors.forename: Sue
authors.initials: S
authors.lastname: Shin
authors.affiliation: Department of Laboratory Medicine, Boramae Medical Center, Seoul National University College of Medicine, Seoul, Korea.
authors.forename: Eun Youn
authors.initials: EY
authors.lastname: Roh
authors.affiliation: Department of Otorhinolaryngology-Head and Neck Surgery, Seoul National University College of Medicine, Seoul, Korea.
authors.forename: Doo Hee
authors.initials: DH
authors.lastname: Han
authors.affiliation: Department of Otorhinolaryngology-Head and Neck Surgery, Boramae Medical Center, Seoul National University College of Medicine, Seoul, Korea.
authors.forename: Dae Woo
authors.initials: DW
authors.lastname: Kim
mesh_terms: D000328:Adult; D039521:CD11c Antigen / Q000378:metabolism*; D002908:Chronic Disease; D047093:Eosinophil Major Basic Protein / Q000378:metabolism*; D005260:Female; D006801:Humans; D007113:Immunity, Innate; D008297:Male; D008875:Middle Aged; D009298:Nasal Polyps / Q000150:complications; D010641:Phenotype; D056910:Republic of Korea; D012852:Sinusitis / Q000150:complications / Q000276:immunology* / Q000378:metabolism
chemical_list: D039521:CD11c Antigen; D047093:Eosinophil Major Basic Protein