title: Lgr4 is required for endometrial receptivity acquired through ovarian hormone signaling.
abstract: Previously, using the Keratin5-Cre transgenic mouse model we reported that female Lgr4-conditional KO mice (Lgr4(K5 KO)) showed subfertility with defective stromal decidualization due to abnormal development of the uterine gland. However, the impact of the LGR4 defect on luminal epithelial cells was not investigated in the previous report. Here, we focused on the receptive state of the luminal epithelium in Lgr4(K5 KO) mice that received ovarian hormone treatment. In Lgr4(K5 KO) mice, progesterone failed to inhibit the luminal epithelial cell proliferation. Immunohistochemical and qRT-PCR analyses revealed down-regulated progesterone signaling in the uterus of Lgr4(K5 KO) mice. These results demonstrated that LGR4 is essential for the acquisition of endometrial receptivity through ovarian hormone signaling.
authors.affiliation: a Laboratory of Molecular Biology, Graduate School of Agricultural Science , Tohoku University , Sendai , Japan.
authors.forename: Tomoyo
authors.initials: T
authors.lastname: Kida
authors.forename: Kazunori
authors.initials: K
authors.lastname: Oyama
authors.forename: Mizuki
authors.initials: M
authors.lastname: Sone
authors.forename: Masae
authors.initials: M
authors.lastname: Koizumi
authors.forename: Shizu
authors.initials: S
authors.lastname: Hidema
authors.forename: Katsuhiko
authors.initials: K
authors.lastname: Nishimori
mesh_terms: D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D004717:Endometrium / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D005260:Female; D005300:Fertility Agents, Female / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects; D051379:Mice; D018345:Mice, Knockout; D011374:Progesterone / Q000235:genetics / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D043562:Receptors, G-Protein-Coupled / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects
chemical_list: D005300:Fertility Agents, Female; C496200:LGR4 protein, mouse; D043562:Receptors, G-Protein-Coupled; D011374:Progesterone
keywords: estrogen receptor; leucine-rich repeat containing G-protein coupled receptor 4 (LGR4); progesterone receptor; uterine receptivity