title: Chitosan-coated poly(lactic-co-glycolic) acid nanoparticles as an efficient delivery system for Newcastle disease virus DNA vaccine.
abstract: We determined the efficacy and safety of chitosan (CS)-coated poly(lactic-co-glycolic) acid (PLGA) nanoparticles (NPs) as a delivery system for a vaccine to protect chickens against Newcastle disease virus (NDV). The newly constructed vaccine contained DNA (the F gene) of NDV. The Newcastle disease virus (NDV) F gene deoxyribonucleic acid (DNA) plasmid (pFDNA)-CS/PLGA-NPs were spherical (diameter =699.1 ± 5.21 nm [mean ± standard deviation]) and smooth, with an encapsulation efficiency of 98.1% and a Zeta potential of +6.35 mV. An in vitro release assay indicated that CS controlled the burst release of plasmid DNA, such that up to 67.4% of the entire quantity of plasmid DNA was steadily released from the pFDNA-CS/PLGA-NPs. An in vitro expression assay indicated that the expression of nanoparticles (NPs) was maintained in the NPs. In an immunization test with specific pathogen-free chickens, the pFDNA-CS/PLGA-NPs induced stronger cellular, humoral, and mucosal immune responses than the plasmid DNA vaccine alone. The pFDNA-CS/PLGA-NPs did not harm 293T cells in an in vitro assay and did not harm chickens in an in vivo assay. Overall, the results indicated that CS-coated PLGA NPs can serve as an efficient and safe mucosal immune delivery system for NDV DNA vaccine.
authors.affiliation: Laboratory of Microbiology, School of Life Science, Heilongjiang University, Harbin, People's Republic of China.
authors.forename: Kai
authors.initials: K
authors.lastname: Zhao
authors.affiliation: Laboratory of Microbiology, School of Life Science, Heilongjiang University, Harbin, People's Republic of China ; Division of Swine Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Harbin, People's Republic of China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Laboratory of Microbiology, School of Life Science, Heilongjiang University, Harbin, People's Republic of China.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Laboratory of Microbiology, School of Life Science, Heilongjiang University, Harbin, People's Republic of China ; Division of Swine Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Harbin, People's Republic of China.
authors.forename: Ci
authors.initials: C
authors.lastname: Shi
authors.affiliation: Laboratory of Microbiology, School of Life Science, Heilongjiang University, Harbin, People's Republic of China.
authors.forename: Xin
authors.initials: X
authors.lastname: Wang
authors.affiliation: Laboratory of Microbiology, School of Life Science, Heilongjiang University, Harbin, People's Republic of China.
authors.forename: Xiaohua
authors.initials: X
authors.lastname: Wang
authors.affiliation: Key Laboratory of Chemical Engineering Process and Technology for High-efficiency Conversion, Heilongjiang University, Harbin, People's Republic of China.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Jin
authors.affiliation: Division of Swine Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Harbin, People's Republic of China.
authors.forename: Shangjin
authors.initials: S
authors.lastname: Cui
mesh_terms: D000818:Animals; D002645:Chickens; D048271:Chitosan / Q000737:chemistry* / Q000493:pharmacokinetics; D004279:DNA, Viral / Q000235:genetics / Q000276:immunology / Q000378:metabolism / Q000493:pharmacokinetics*; D004337:Drug Carriers / Q000737:chemistry* / Q000493:pharmacokinetics; D007070:Immunoglobulin A / Q000032:analysis; D019344:Lactic Acid / Q000737:chemistry* / Q000493:pharmacokinetics; D008214:Lymphocytes / Q000276:immunology; D053758:Nanoparticles / Q000737:chemistry*; D009521:Newcastle Disease / Q000517:prevention & control / Q000821:virology; D009522:Newcastle disease virus / Q000235:genetics*; D011100:Polyglycolic Acid / Q000737:chemistry* / Q000493:pharmacokinetics; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D013666:Tears / Q000737:chemistry; D019444:Vaccines, DNA / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology / Q000493:pharmacokinetics*
chemical_list: D004279:DNA, Viral; D004337:Drug Carriers; D007070:Immunoglobulin A; D019444:Vaccines, DNA; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D048271:Chitosan
keywords: immune effect; mucosal immune delivery system