title: Synthesis of coenzyme A thioesters using methyl acyl phosphates in an aqueous medium.
abstract: Regioselective S-acylation of coenzyme A (CoA) is achieved under aqueous conditions using various aliphatic and aromatic carboxylic acids activated as their methyl acyl phosphate monoesters. Unlike many hydrophobic activating groups, the anionic methyl acyl phosphate mixed anhydride is more compatible with aqueous solvents, making it useful for conducting acylation reactions in an aqueous medium.
authors.affiliation: Department of Biochemistry and Molecular Biology, Dalhousie University, Halifax, NS B3H 4R2, Canada. sbearne@dal.ca.
authors.forename: Mohan
authors.initials: M
authors.lastname: Pal
authors.forename: Stephen L
authors.initials: SL
authors.lastname: Bearne
mesh_terms: D003065:Coenzyme A / Q000737:chemistry* / Q000378:metabolism; D004952:Esters / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism; D015394:Molecular Structure; D010710:Phosphates / Q000737:chemistry* / Q000378:metabolism; D013438:Sulfhydryl Compounds / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism; D014867:Water / Q000737:chemistry / Q000378:metabolism
chemical_list: D004952:Esters; D010710:Phosphates; D013438:Sulfhydryl Compounds; D014867:Water; D003065:Coenzyme A