title: Goniometer-based femtosecond crystallography with X-ray free electron lasers.
abstract: The emerging method of femtosecond crystallography (FX) may extend the diffraction resolution accessible from small radiation-sensitive crystals and provides a means to determine catalytically accurate structures of acutely radiation-sensitive metalloenzymes. Automated goniometer-based instrumentation developed for use at the Linac Coherent Light Source enabled efficient and flexible FX experiments to be performed on a variety of sample types. In the case of rod-shaped Cpl hydrogenase crystals, only five crystals and about 30 min of beam time were used to obtain the 125 still diffraction patterns used to produce a 1.6-Å resolution electron density map. For smaller crystals, high-density grids were used to increase sample throughput; 930 myoglobin crystals mounted at random orientation inside 32 grids were exposed, demonstrating the utility of this approach. Screening results from cryocooled crystals of β2-adrenoreceptor and an RNA polymerase II complex indicate the potential to extend the diffraction resolution obtainable from very radiation-sensitive samples beyond that possible with undulator-based synchrotron sources.
authors.affiliation: Stanford Synchrotron Radiation Lightsource, HodgsonK@stanford.edu acohen@slac.stanford.edu.
authors.forename: Aina E
authors.initials: AE
authors.lastname: Cohen
authors.affiliation: Stanford Synchrotron Radiation Lightsource.
authors.forename: S Michael
authors.initials: SM
authors.lastname: Soltis
authors.affiliation: Stanford Synchrotron Radiation Lightsource.
authors.forename: Ana
authors.initials: A
authors.lastname: González
authors.affiliation: Stanford Synchrotron Radiation Lightsource.
authors.forename: Laura
authors.initials: L
authors.lastname: Aguila
authors.affiliation: Linac Coherent Light Source, and.
authors.forename: Roberto
authors.initials: R
authors.lastname: Alonso-Mori
authors.affiliation: Department of Structural Biology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261;
authors.forename: Christopher O
authors.initials: CO
authors.lastname: Barnes
authors.affiliation: Stanford Synchrotron Radiation Lightsource, Departments of Chemistry.
authors.forename: Elizabeth L
authors.initials: EL
authors.lastname: Baxter
authors.affiliation: Stanford Synchrotron Radiation Lightsource.
authors.forename: Winnie
authors.initials: W
authors.lastname: Brehmer
authors.affiliation: Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720;
authors.forename: Aaron S
authors.initials: AS
authors.lastname: Brewster
authors.affiliation: Molecular and Cellular Physiology, and Howard Hughes Medical Institute, Stanford University, Stanford, CA 94305;
authors.forename: Axel T
authors.initials: AT
authors.lastname: Brunger
authors.affiliation: Department of Structural Biology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261;
authors.forename: Guillermo
authors.initials: G
authors.lastname: Calero
authors.affiliation: Stanford Synchrotron Radiation Lightsource.
authors.forename: Joseph F
authors.initials: JF
authors.lastname: Chang
authors.affiliation: Linac Coherent Light Source, and.
authors.forename: Matthieu
authors.initials: M
authors.lastname: Chollet
authors.affiliation: Stanford Synchrotron Radiation Lightsource.
authors.forename: Paul
authors.initials: P
authors.lastname: Ehrensberger
authors.affiliation: Stanford Synchrotron Radiation Lightsource.
authors.forename: Thomas L
authors.initials: TL
authors.lastname: Eriksson
authors.affiliation: Linac Coherent Light Source, and.
authors.forename: Yiping
authors.initials: Y
authors.lastname: Feng
authors.affiliation: Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720;
authors.forename: Johan
authors.initials: J
authors.lastname: Hattne
authors.affiliation: Stanford Synchrotron Radiation Lightsource.
authors.forename: Britt
authors.initials: B
authors.lastname: Hedman
authors.affiliation: Stanford Synchrotron Radiation Lightsource.
authors.forename: Michael
authors.initials: M
authors.lastname: Hollenbeck
authors.affiliation: Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720; Department of Biochemistry and Biophysics, University of California, San Francisco, CA 94158; and.
authors.forename: James M
authors.initials: JM
authors.lastname: Holton
authors.affiliation: Department of Chemistry and Biochemistry, Montana State University, Bozeman, MT 59715.
authors.forename: Stephen
authors.initials: S
authors.lastname: Keable
authors.affiliation: Molecular and Cellular Physiology, and.
authors.forename: Brian K
authors.initials: BK
authors.lastname: Kobilka
authors.affiliation: Stanford Synchrotron Radiation Lightsource.
authors.forename: Elena G
authors.initials: EG
authors.lastname: Kovaleva
authors.affiliation: Molecular and Cellular Physiology, and.
authors.forename: Andrew C
authors.initials: AC
authors.lastname: Kruse
authors.affiliation: Linac Coherent Light Source, and.
authors.forename: Henrik T
authors.initials: HT
authors.lastname: Lemke
authors.affiliation: Department of Structural Biology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261;
authors.forename: Guowu
authors.initials: G
authors.lastname: Lin
authors.affiliation: Molecular and Cellular Physiology, and Howard Hughes Medical Institute, Stanford University, Stanford, CA 94305;
authors.forename: Artem Y
authors.initials: AY
authors.lastname: Lyubimov
authors.affiliation: Molecular and Cellular Physiology, and.
authors.forename: Aashish
authors.initials: A
authors.lastname: Manglik
authors.affiliation: Stanford Synchrotron Radiation Lightsource.
authors.forename: Irimpan I
authors.initials: II
authors.lastname: Mathews
authors.affiliation: Stanford Synchrotron Radiation Lightsource.
authors.forename: Scott E
authors.initials: SE
authors.lastname: McPhillips
authors.affiliation: Linac Coherent Light Source, and.
authors.forename: Silke
authors.initials: S
authors.lastname: Nelson
authors.affiliation: Department of Chemistry and Biochemistry, Montana State University, Bozeman, MT 59715.
authors.forename: John W
authors.initials: JW
authors.lastname: Peters
authors.affiliation: Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720;
authors.forename: Nicholas K
authors.initials: NK
authors.lastname: Sauter
authors.affiliation: Stanford Synchrotron Radiation Lightsource.
authors.forename: Clyde A
authors.initials: CA
authors.lastname: Smith
authors.affiliation: Stanford Synchrotron Radiation Lightsource.
authors.forename: Jinhu
authors.initials: J
authors.lastname: Song
authors.affiliation: Department of Structural Biology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261;
authors.forename: Hilary P
authors.initials: HP
authors.lastname: Stevenson
authors.affiliation: Stanford Synchrotron Radiation Lightsource, Departments of Chemistry.
authors.forename: Yingssu
authors.initials: Y
authors.lastname: Tsai
authors.affiliation: Molecular and Cellular Physiology, and Howard Hughes Medical Institute, Stanford University, Stanford, CA 94305;
authors.forename: Monarin
authors.initials: M
authors.lastname: Uervirojnangkoorn
authors.affiliation: Stanford Synchrotron Radiation Lightsource.
authors.forename: Vladimir
authors.initials: V
authors.lastname: Vinetsky
authors.affiliation: Photon Science, SLAC National Accelerator Laboratory, Stanford University, Menlo Park, CA 94025; Structural Biology, and.
authors.forename: Soichi
authors.initials: S
authors.lastname: Wakatsuki
authors.affiliation: Molecular and Cellular Physiology, and Structural Biology, and.
authors.forename: William I
authors.initials: WI
authors.lastname: Weis
authors.affiliation: Department of Chemistry and Biochemistry, Montana State University, Bozeman, MT 59715.
authors.forename: Oleg A
authors.initials: OA
authors.lastname: Zadvornyy
authors.affiliation: Molecular and Cellular Physiology, and Howard Hughes Medical Institute, Stanford University, Stanford, CA 94305;
authors.forename: Oliver B
authors.initials: OB
authors.lastname: Zeldin
authors.affiliation: Linac Coherent Light Source, and.
authors.forename: Diling
authors.initials: D
authors.lastname: Zhu
authors.affiliation: Stanford Synchrotron Radiation Lightsource, Departments of Chemistry, HodgsonK@stanford.edu acohen@slac.stanford.edu.
authors.forename: Keith O
authors.initials: KO
authors.lastname: Hodgson
mesh_terms: D002627:Chemistry, Physical / Q000295:instrumentation*; D003460:Crystallization; D018360:Crystallography, X-Ray / Q000379:methods*; D004583:Electrons; D007834:Lasers; D008958:Models, Molecular; D009211:Myoglobin / Q000737:chemistry; D011487:Protein Conformation; D011506:Proteins / Q000737:chemistry*; D012319:RNA Polymerase II / Q000737:chemistry; D018343:Receptors, Adrenergic, beta-2 / Q000737:chemistry; D015203:Reproducibility of Results; D017356:Synchrotrons; D014961:X-Ray Diffraction / Q000379:methods; D014965:X-Rays
chemical_list: D009211:Myoglobin; D011506:Proteins; D018343:Receptors, Adrenergic, beta-2; D012319:RNA Polymerase II
keywords: XFEL; crystallography; femtosecond diffraction; structural biology