title: microRNA-dependent temporal gene expression in the ureteric bud epithelium during mammalian kidney development.
abstract: BACKGROUND
  Our previous study on mouse mutants with the ureteric bud (UB) epithelium-specific Dicer deletion (Dicer UB mutants) demonstrated the significance of UB epithelium-derived miRNAs in UB development.
  
  
  RESULTS
  Our whole-genome transcriptional profiling showed that the Dicer mutant UB epithelium abnormally retained transcriptional features of the early UB epithelium and failed to express many genes associated with collecting duct differentiation. Furthermore, we identified a temporal expression pattern of early UB genes during UB epithelium development in which gene expression was detected at early developmental stages and became undetectable by embryonic day 14.5. In contrast, expression of early UB genes persisted at later stages in the Dicer mutant UB epithelium and increased at early stages. Our bioinformatic analysis of the abnormally persistently expressed early genes in the Dicer mutant UB epithelium showed significant enrichment of the let-7 family miRNA targets. We further identified a temporal expression pattern of let-7 miRNAs in the UB epithelium that is anti-parallel to that of some early UB genes during kidney development.
  
  
  CONCLUSIONS
  We propose a model in which the let-7 family miRNAs silence the expression of a subset of early genes in the UB epithelium at later developmental stages to promote collecting duct differentiation. Developmental Dynamics 244:444-456, 2015. © 2014 Wiley Periodicals, Inc.
authors.affiliation: Department of Cell Biology and Child Health Research Center, University of Virginia School of Medicine, Charlottesville, Virginia.
authors.forename: Vidya K
authors.initials: VK
authors.lastname: Nagalakshmi
authors.forename: Volkhard
authors.initials: V
authors.lastname: Lindner
authors.forename: Andy
authors.initials: A
authors.lastname: Wessels
authors.forename: Jing
authors.initials: J
authors.lastname: Yu
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000502:physiology*; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D007685:Kidney Tubules, Collecting / Q000166:cytology / Q000196:embryology*; D051379:Mice; D018345:Mice, Knockout; D035683:MicroRNAs / Q000096:biosynthesis* / Q000235:genetics; D059467:Transcriptome / Q000502:physiology*; D019459:Urothelium / Q000166:cytology / Q000196:embryology*
chemical_list: D035683:MicroRNAs; C542687:mirnlet7 microRNA, mouse
keywords: collecting duct differentiation; let-7; miRNAs; mouse