title: Comparative transcriptional profiling of two wheat genotypes, with contrasting levels of minerals in grains, shows expression differences during grain filling.
abstract: Wheat is one of the most important cereal crops in the world. To identify the candidate genes for mineral accumulation, it is important to examine differential transcriptome between wheat genotypes, with contrasting levels of minerals in grains. A transcriptional comparison of developing grains was carried out between two wheat genotypes- Triticum aestivum Cv. WL711 (low grain mineral), and T. aestivum L. IITR26 (high grain mineral), using Affymetrix GeneChip Wheat Genome Array. The study identified a total of 580 probe sets as differentially expressed (with log2 fold change of ≥2 at p≤0.01) between the two genotypes, during grain filling. Transcripts with significant differences in induction or repression between the two genotypes included genes related to metal homeostasis, metal tolerance, lignin and flavonoid biosynthesis, amino acid and protein transport, vacuolar-sorting receptor, aquaporins, and stress responses. Meta-analysis revealed spatial and temporal signatures of a majority of the differentially regulated transcripts.
authors.affiliation: National Agri-Food Biotechnology Institute, Department of Biotechnology (DBT), C-127, Industrial Area, Phase VIII, Mohali, 160071, India.
authors.forename: Sudhir P
authors.initials: SP
authors.lastname: Singh
authors.affiliation: National Agri-Food Biotechnology Institute, Department of Biotechnology (DBT), C-127, Industrial Area, Phase VIII, Mohali, 160071, India.
authors.forename: Raja
authors.initials: R
authors.lastname: Jeet
authors.affiliation: National Agri-Food Biotechnology Institute, Department of Biotechnology (DBT), C-127, Industrial Area, Phase VIII, Mohali, 160071, India.
authors.forename: Jitendra
authors.initials: J
authors.lastname: Kumar
authors.affiliation: National Agri-Food Biotechnology Institute, Department of Biotechnology (DBT), C-127, Industrial Area, Phase VIII, Mohali, 160071, India.
authors.forename: Vishnu
authors.initials: V
authors.lastname: Shukla
authors.affiliation: School of Biotechnology, Banaras Hindu University, Varanasi, India.
authors.forename: Rakesh
authors.initials: R
authors.lastname: Srivastava
authors.affiliation: National Agri-Food Biotechnology Institute, Department of Biotechnology (DBT), C-127, Industrial Area, Phase VIII, Mohali, 160071, India.
authors.forename: Shrikant S
authors.initials: SS
authors.lastname: Mantri
authors.affiliation: National Agri-Food Biotechnology Institute, Department of Biotechnology (DBT), C-127, Industrial Area, Phase VIII, Mohali, 160071, India.
authors.forename: Rakesh
authors.initials: R
authors.lastname: Tuli
mesh_terms: D001483:Base Sequence; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant / Q000502:physiology*; D008903:Minerals / Q000378:metabolism*; D008969:Molecular Sequence Data; D012639:Seeds / Q000235:genetics / Q000378:metabolism*; D013045:Species Specificity; D014158:Transcription, Genetic / Q000502:physiology*; D014908:Triticum / Q000235:genetics / Q000378:metabolism*
chemical_list: D008903:Minerals