title: Biocompatibility of core@shell particles: cytotoxicity and genotoxicity in human osteosarcoma cells of colloidal silica spheres coated with crystalline or amorphous zirconia.
abstract: The cytotoxicity and genotoxicity of novel colloidal silica spheres coated with crystalline or amorphous zirconia (SiO2@ZrO2(cryst) or SiO2@ZrO2(am)) have been studied in a human osteosarcoma cell line (MG-63), after 24 h exposure. SiO2@ZrO2(cryst) and SiO2@ZrO2(am) had mean diameters of 782±19 and 891±34 nm, respectively. SiO2@ZrO2(cryst) exposure reduced cell viability, with an increase in reactive oxygen species (ROS) and a decrease of the GSH/GSSG ratio. The comet and micronucleus (MN) assays detected DNA damage at 5 and 25 μg/mL, respectively. SiO2@ZrO2(am) induced genotoxic action only at 10 and 50 μg/mL (comet and MN assays), along with a decrease of the GSH/GSSG ratio at 50 μg/mL. Both particles were found inside the cells, forming vesicles; however, none of them entered the nucleus. Our findings show that crystallization of the shell of the amorphous ZrO2 increases both cytotoxicity and genotoxicity.
authors.affiliation: CEQUINOR (Centro de Química Inorgánica), Facultad de Ciencias Exactas, UNLP. 47 y 115 (1900), La Plata, Argentina; Cátedra de Bioquímica Patológica, Facultad de Ciencias Exactas, UNLP. 47 y 115 (1900), La Plata, Argentina. Electronic address: aldivirgilio@biol.unlp.edu.ar.
authors.forename: A L
authors.initials: AL
authors.lastname: Di Virgilio
authors.affiliation: CETMIC (Centro de Tecnología de Recursos Minerales y Cerámica), Cno Centenario y 506, CC 49 (B1897ZCA), M.B. Gonnet, Prov. Buenos Aires, Argentina.
authors.forename: P M
authors.initials: PM
authors.lastname: Arnal
authors.affiliation: Cátedra de Bioquímica Patológica, Facultad de Ciencias Exactas, UNLP. 47 y 115 (1900), La Plata, Argentina; CETMIC (Centro de Tecnología de Recursos Minerales y Cerámica), Cno Centenario y 506, CC 49 (B1897ZCA), M.B. Gonnet, Prov. Buenos Aires, Argentina.
authors.forename: I
authors.initials: I
authors.lastname: Maisuls
mesh_terms: D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D055598:Chemical Phenomena; D020552:Comet Assay; D004249:DNA Damage / Q000187:drug effects*; D006801:Humans; D008422:Materials Testing / Q000379:methods*; D015162:Micronucleus Tests; D012516:Osteosarcoma / Q000378:metabolism; D010316:Particle Size; D017382:Reactive Oxygen Species / Q000378:metabolism; D012822:Silicon Dioxide / Q000494:pharmacology*; D015040:Zirconium / Q000737:chemistry*
chemical_list: D017382:Reactive Oxygen Species; D012822:Silicon Dioxide; D015040:Zirconium; C028541:zirconium oxide
keywords: Core@shell materials; Cytotoxicity; Genotoxicity; Human osteosarcoma cells; Silica; Zirconia