title: Cupric oxide nanoparticles-enhanced chemiluminescence method for measurement of β-lactam antibiotics.
abstract: A simple, sensitive cupric oxide nanoparticles (CuO NPs) enhanced chemiluminescence (CL) method was developed for the measurement of β-lactam antibiotics, including amoxicillin and cefazolin sodium. The method was based on suppression of the CuO NPs-luminol-H2O2 CL reaction by β-lactam antibiotics. Experimental parameters that influenced the inhibitory effect of the antibiotic drugs on the CL system, such as NaOH (mol/L), luminol (µmol/L), H2O2 (mol/L) and CuO NPs (mg/L) concentrations, were optimized. Calibration graphs were linear and had dynamic ranges of 1.0 × 10(-6) to 8.0 × 10(-6) mol/L and 3.0 × 10(-5) to 5.0 × 10(-3) mol/L for amoxicillin and cefazolin sodium, respectively, with corresponding detection limits of 7.9 × 10(-7) mol/L and 1.8 × 10(-5) mol/L. The relative standard deviations of five replicate measurements of 5.0 × 10(-6) amoxicillin and 5 × 10(-4) cefazolin sodium were 5.43 and 5.01%, respectively. The synthesized CuO NPs were characterized by X-ray diffraction (XRD) and transmission electronmicroscopy (TEM). The developed approach was exploited successfully to measure antibiotics in pharmaceutical preparations.
authors.affiliation: Department of Chemistry, Faculty of Science, University of Maragheh, Maragheh, Iran.
authors.forename: Mortaza
authors.initials: M
authors.lastname: Iranifam
authors.affiliation: Department of Chemistry, Faculty of Science, University of Maragheh, Maragheh, Iran.
authors.forename: Merhnaz
authors.initials: M
authors.lastname: Khabbaz Kharameh
mesh_terms: D000658:Amoxicillin / Q000032:analysis; D000900:Anti-Bacterial Agents / Q000032:analysis*; D002437:Cefazolin / Q000032:analysis; D003300:Copper / Q000737:chemistry*; D006861:Hydrogen Peroxide / Q000737:chemistry; D057230:Limit of Detection; D008163:Luminescent Measurements / Q000379:methods*; D008165:Luminol / Q000737:chemistry; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles / Q000737:chemistry*; D014961:X-Ray Diffraction; D047090:beta-Lactams / Q000032:analysis*
chemical_list: D000900:Anti-Bacterial Agents; D047090:beta-Lactams; D008165:Luminol; D003300:Copper; D000658:Amoxicillin; D006861:Hydrogen Peroxide; D002437:Cefazolin; C030973:cupric oxide
keywords: amoxicillin; cefazolin sodium; chemiluminescence; cupric oxide nanoparticles; luminol