title: Perforin, CD28 and CD95 expression in circulating CD4 and CD8 cells as predictors of head and neck (H&N) cancer patient survival.
abstract: Long-term survival of H&N cancer patients has not improved significantly over the last 30 years. The possibility of using circulating blood cell phenotypes as a prognostic biomarker of H&N cancer patient was investigated in this study. Pre-treatment, circulating T lymphocyte subpopulations as well as the survival time of the patients in question were studied. Upregulated CD4+ perforin+ and CD8+ CD95+ but downregulated CD4+ CD28+ (p < 0.001) were detected in H&N cancer patients. With 3 years of follow-up time, an increase in the frequency of the pre-treatment, circulating CD4+ perforin+ cells and CD8+ perforin+ cells was showed to have reverse effects on the survival time in H&N cancer patients (p < 0.01). Detection of perforin+ frequency in CD4+ and CD8+ lymphocyte by FACS is fast, simple and cost-effective. A potential role of perforin expression in CD4+ and CD8+ cells as a prognostic biomarker for H&N cancer patient in the clinical setting was suggested.
authors.affiliation: Department of Laboratory Medicine, Ryhov Hospital, SE-55185, Jönköping, Sweden, nongnit.lewin@lj.se.
authors.forename: Nongnit
authors.initials: N
authors.lastname: Laytragoon-Lewin
authors.forename: Fredrik
authors.initials: F
authors.lastname: Jönson
authors.forename: Jan
authors.initials: J
authors.lastname: Lundgren
authors.forename: Lars Erik
authors.initials: LE
authors.lastname: Rutqvist
authors.forename: Anders
authors.initials: A
authors.lastname: Wikby
authors.forename: Sture
authors.initials: S
authors.lastname: Löfgren
authors.forename: Freddi
authors.initials: F
authors.lastname: Lewin
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000097:blood; D018106:CD28 Antigens / Q000097:blood*; D015496:CD4-Positive T-Lymphocytes / Q000378:metabolism*; D018414:CD8-Positive T-Lymphocytes / Q000378:metabolism*; D005260:Female; D005500:Follow-Up Studies; D015972:Gene Expression Regulation, Neoplastic; D006258:Head and Neck Neoplasms / Q000097:blood* / Q000175:diagnosis / Q000401:mortality; D006801:Humans; D008297:Male; D008875:Middle Aged; D009360:Neoplastic Cells, Circulating / Q000378:metabolism; D054353:Perforin / Q000097:blood*; D011237:Predictive Value of Tests; D015996:Survival Rate / Q000639:trends; D019014:fas Receptor / Q000097:blood*
chemical_list: D014408:Biomarkers, Tumor; D018106:CD28 Antigens; C491901:FAS protein, human; D019014:fas Receptor; D054353:Perforin