title: Promiscuous activity of (S,S)-butanediol dehydrogenase is responsible for glycerol production from 1,3-dihydroxyacetone in Corynebacterium glutamicum under oxygen-deprived conditions.
abstract: Corynebacterium glutamicum can consume glucose to excrete glycerol under oxygen deprivation. Although glycerol synthesis from 1,3-dihydroxyacetone (DHA) has been speculated, no direct evidence has yet been provided in C. glutamicum. Enzymatic and genetic investigations here indicate that the glycerol is largely produced from DHA and, unexpectedly, the reaction is catalyzed by (S,S)-butanediol dehydrogenase (ButA) that inherently catalyzes the interconversion between S-acetoin and (S,S)-2,3-butanediol. Consequently, the following pathway for glycerol biosynthesis in the bacterium emerges: dihydroxyacetone phosphate is dephosphorylated by HdpA to DHA, which is subsequently reduced to glycerol by ButA. This study emphasizes the importance of promiscuous activity of the enzyme in vivo.
authors.affiliation: Research Institute of Innovative Technology for the Earth, 9-2, Kizugawadai, Kizugawa, Kyoto, 619-0292, Japan.
authors.forename: Toru
authors.initials: T
authors.lastname: Jojima
authors.forename: Takafumi
authors.initials: T
authors.lastname: Igari
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Moteki
authors.forename: Masako
authors.initials: M
authors.lastname: Suda
authors.forename: Hideaki
authors.initials: H
authors.lastname: Yukawa
authors.forename: Masayuki
authors.initials: M
authors.lastname: Inui
mesh_terms: D000429:Alcohol Oxidoreductases / Q000235:genetics / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D048230:Corynebacterium glutamicum / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism; D004098:Dihydroxyacetone / Q000378:metabolism; D005990:Glycerol / Q000378:metabolism*; D010100:Oxygen / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D000429:Alcohol Oxidoreductases; C019801:butanediol dehydrogenase; D004098:Dihydroxyacetone; D005990:Glycerol; D010100:Oxygen