title: Penicillium janthinellum: a source of efficient and high levels of β-glucosidase.
abstract: Penicillium janthinellum strain isolated from leaf litters of oak trees from montane alpine forests of Shivalik hills (India) produced high levels of β-glucosidase both during solid-state fermentation (796 units/gds) and shake flask cultures (65.3 units/ml). The peptide mass fingerprinting of the secretome showed a variety of glycosyl hydrolases. β-Glucosidase was purified and characterized to be a GH3 family member that had a molecular weight (M r) of 101 kDa and pI of 4.5. β-Glucosidase was optimally active at 60 °C at pH 5.0 but showed appreciable activity and thermostability under alkaline conditions (pH 9.0) also. β-Glucosidase activity was positively modulated in the presence of Mn(2+) ions. The enzyme preferentially catalyzed the hydrolysis of p-nitrophenol-β-D-glucopyranoside (pNPG) but also recognized cellobiose as substrates. K m and V max for the hydrolysis of pNPG by β-glucosidase were calculated as 3.3 mM and 444 μmol min(-1) mg protein(-1). Purified β-glucosidase showed transglycosylation activity in the presence of methanol as an acceptor molecule.
authors.affiliation: Department of Microbiology, Guru Nanak Dev University, Amritsar, Punjab, 143005, India, amandeep.aurora@gmail.com.
authors.forename: Amandeep
authors.initials: A
authors.lastname: Kaur
authors.forename: B S
authors.initials: BS
authors.lastname: Chadha
mesh_terms: D004795:Enzyme Stability; D065928:Forests; D006031:Glycosylation; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008670:Metals / Q000494:pharmacology; D010407:Penicillium / Q000201:enzymology / Q000378:metabolism*; D018515:Plant Leaves / Q000378:metabolism; D029963:Quercus / Q000382:microbiology; D013379:Substrate Specificity; D013696:Temperature; D001617:beta-Glucosidase / Q000096:biosynthesis* / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D008670:Metals; D001617:beta-Glucosidase