title: Combined use of Mycobacterium tuberculosis-specific CD4 and CD8 T-cell responses is a powerful diagnostic tool of active tuberculosis.
abstract: Immune-based assays are promising tools to help to formulate diagnosis of active tuberculosis. A multiparameter flow cytometry assay assessing T-cell responses specific to Mycobacterium tuberculosis and the combination of both CD4 and CD8 T-cell responses accurately discriminated between active tuberculosis and latent infection.
authors.affiliation: Division of Immunology and Allergy, Lausanne University Hospital, Switzerland South African Tuberculosis Vaccine Initiative, Institute of Infectious Diseases and Molecular Medicine, University of Cape Town, South Africa.
authors.forename: Virginie
authors.initials: V
authors.lastname: Rozot
authors.affiliation: Division of Immunology and Allergy, Lausanne University Hospital, Switzerland.
authors.forename: Amelio
authors.initials: A
authors.lastname: Patrizia
authors.affiliation: Division of Immunology and Allergy, Lausanne University Hospital, Switzerland.
authors.forename: Selena
authors.initials: S
authors.lastname: Vigano
authors.affiliation: Division of Pneumology.
authors.forename: Jesica
authors.initials: J
authors.lastname: Mazza-Stalder
authors.affiliation: Division of Immunology and Allergy, Lausanne University Hospital, Switzerland.
authors.forename: Elita
authors.initials: E
authors.lastname: Idrizi
authors.affiliation: South African Tuberculosis Vaccine Initiative, Institute of Infectious Diseases and Molecular Medicine, University of Cape Town, South Africa.
authors.forename: Cheryl L
authors.initials: CL
authors.lastname: Day
authors.affiliation: Division of Immunology and Allergy, Lausanne University Hospital, Switzerland.
authors.forename: Matthieu
authors.initials: M
authors.lastname: Perreau
authors.affiliation: Occupational Medicine, Centre Hospitalier Universitaire Vaudois, University of Lausanne, Switzerland.
authors.forename: Catherine
authors.initials: C
authors.lastname: Lazor-Blanchet
authors.affiliation: Division of Immunology and Allergy, Lausanne University Hospital, Switzerland.
authors.forename: Khalid
authors.initials: K
authors.lastname: Ohmiti
authors.affiliation: National Institute for Infectious Diseases, Rome, Italy.
authors.forename: Delia
authors.initials: D
authors.lastname: Goletti
authors.affiliation: Division of Immunology and Allergy, Lausanne University Hospital, Switzerland.
authors.forename: Pierre-Alexandre
authors.initials: PA
authors.lastname: Bart
authors.affiliation: South African Tuberculosis Vaccine Initiative, Institute of Infectious Diseases and Molecular Medicine, University of Cape Town, South Africa.
authors.forename: Willem
authors.initials: W
authors.lastname: Hanekom
authors.affiliation: South African Tuberculosis Vaccine Initiative, Institute of Infectious Diseases and Molecular Medicine, University of Cape Town, South Africa.
authors.forename: Thomas J
authors.initials: TJ
authors.lastname: Scriba
authors.affiliation: Division of Pneumology.
authors.forename: Laurent
authors.initials: L
authors.lastname: Nicod
authors.affiliation: Division of Immunology and Allergy, Lausanne University Hospital, Switzerland Swiss Vaccine Research Institute, Lausanne, Switzerland.
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Pantaleo
authors.affiliation: Division of Immunology and Allergy, Lausanne University Hospital, Switzerland Swiss Vaccine Research Institute, Lausanne, Switzerland.
authors.forename: Alexandre
authors.initials: A
authors.lastname: Harari
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000942:Antigens, Bacterial / Q000276:immunology*; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D002648:Child; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D008297:Male; D008875:Middle Aged; D009169:Mycobacterium tuberculosis / Q000276:immunology*; D014376:Tuberculosis / Q000175:diagnosis* / Q000276:immunology; D055815:Young Adult
chemical_list: D000942:Antigens, Bacterial
keywords: CD8 T cells; active tuberculosis disease; diagnosis; functional profile; latent Mtb infection