title: Surface roughness dependent osteoblast and fibroblast response on poly(L-lactide) films and electrospun membranes.
abstract: Poly(l-lactide) electrospun mats with random and aligned fiber orientation and films have been produced with degrees of crystallinity ranging from 0 up to nearly 50%. The overall surface roughness is practically constant irrespective of the sampling areas (1 × 1 µm to 20 × 20 µm) for degrees of crystallinity below 30%, increasing for higher degrees of crystallinity for the larger sampling areas. Further, due to fiber confinement, surface roughness variations are smaller in electrospun mats. Samples with 50% of crystallinity show the lowest osteoblast and the highest fibroblast proliferation. Therefore, it is verified that higher roughness promotes lower osteoblast but higher fibroblast proliferation. The overall results indicate the relevant role of the sub-microenvironment variations associated to the microscale roughness in determining the different cell responses.
authors.affiliation: Centro/Departamento de Física da Universidade do Minho, Universidade do Minho, Campus de Gualtar, 4710-057, Braga, Portugal.|INL-International Iberian Nanotechnology Laboratory, 4715-330, Braga, Portugal.
authors.forename: Clarisse
authors.initials: C
authors.lastname: Ribeiro
authors.affiliation: Centro/Departamento de Física da Universidade do Minho, Universidade do Minho, Campus de Gualtar, 4710-057, Braga, Portugal.|Instituto Politécnico do Cávado e do Ave, Campus do IPCA, 4750-810, Barcelos, Portugal.
authors.forename: Vitor
authors.initials: V
authors.lastname: Sencadas
authors.affiliation: Centro/Departamento de Física da Universidade do Minho, Universidade do Minho, Campus de Gualtar, 4710-057, Braga, Portugal.
authors.forename: Anabela C
authors.initials: AC
authors.lastname: Areias
authors.affiliation: IBB-Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, Universidade do Minho, Campus de Gualtar, 4710-057, Braga, Portugal.
authors.forename: F Miguel
authors.initials: FM
authors.lastname: Gama
authors.affiliation: Centro/Departamento de Física da Universidade do Minho, Universidade do Minho, Campus de Gualtar, 4710-057, Braga, Portugal.|INL-International Iberian Nanotechnology Laboratory, 4715-330, Braga, Portugal.
authors.forename: Senentxu
authors.initials: S
authors.lastname: Lanceros-Méndez
mesh_terms: D016475:3T3 Cells; D000818:Animals; D049109:Cell Proliferation; D003460:Crystallization; D005347:Fibroblasts / Q000166:cytology*; D051379:Mice; D010006:Osteoblasts / Q000166:cytology*; D011091:Polyesters / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D011091:Polyesters; C033616:poly(lactide)
keywords: cell-material interface; poly(l-lactic acid); tissue and biomedical engineering