title: Overexpression of a soybean ariadne-like ubiquitin ligase gene GmARI1 enhances aluminum tolerance in Arabidopsis.
abstract: Ariadne (ARI) subfamily of RBR (Ring Between Ring fingers) proteins have been found as a group of putative E3 ubiquitin ligases containing RING (Really Interesting New Gene) finger domains in fruitfly, mouse, human and Arabidopsis. Recent studies showed several RING-type E3 ubiquitin ligases play important roles in plant response to abiotic stresses, but the function of ARI in plants is largely unknown. In this study, an ariadne-like E3 ubiquitin ligase gene was isolated from soybean, Glycine max (L.) Merr., and designated as GmARI1. It encodes a predicted protein of 586 amino acids with a RBR supra-domain. Subcellular localization studies using Arabidopsis protoplast cells indicated GmARI protein was located in nucleus. The expression of GmARI1 in soybean roots was induced as early as 2-4 h after simulated stress treatments such as aluminum, which coincided with the fact of aluminum toxicity firstly and mainly acting on plant roots. In vitro ubiquitination assay showed GmARI1 protein has E3 ligase activity. Overexpression of GmARI1 significantly enhanced the aluminum tolerance of transgenic Arabidopsis. These findings suggest that GmARI1 encodes a RBR type E3 ligase, which may play important roles in plant tolerance to aluminum stress.
authors.affiliation: National Key Laboratory of Crop Genetics and Germplasm Enhancement, National Center for Soybean Improvement, Key Laboratory for Biology and Genetic Improvement of Soybean (General, Ministry of Agriculture), Nanjing Agricultural University, Nanjing, Jiangsu, China.
authors.forename: Xiaolian
authors.initials: X
authors.lastname: Zhang
authors.affiliation: National Key Laboratory of Crop Genetics and Germplasm Enhancement, National Center for Soybean Improvement, Key Laboratory for Biology and Genetic Improvement of Soybean (General, Ministry of Agriculture), Nanjing Agricultural University, Nanjing, Jiangsu, China.
authors.forename: Ning
authors.initials: N
authors.lastname: Wang
authors.affiliation: National Key Laboratory of Crop Genetics and Germplasm Enhancement, National Center for Soybean Improvement, Key Laboratory for Biology and Genetic Improvement of Soybean (General, Ministry of Agriculture), Nanjing Agricultural University, Nanjing, Jiangsu, China.
authors.forename: Pei
authors.initials: P
authors.lastname: Chen
authors.affiliation: National Key Laboratory of Crop Genetics and Germplasm Enhancement, National Center for Soybean Improvement, Key Laboratory for Biology and Genetic Improvement of Soybean (General, Ministry of Agriculture), Nanjing Agricultural University, Nanjing, Jiangsu, China.
authors.forename: Mengmeng
authors.initials: M
authors.lastname: Gao
authors.affiliation: National Key Laboratory of Crop Genetics and Germplasm Enhancement, National Center for Soybean Improvement, Key Laboratory for Biology and Genetic Improvement of Soybean (General, Ministry of Agriculture), Nanjing Agricultural University, Nanjing, Jiangsu, China.
authors.forename: Juge
authors.initials: J
authors.lastname: Liu
authors.affiliation: National Key Laboratory of Crop Genetics and Germplasm Enhancement, National Center for Soybean Improvement, Key Laboratory for Biology and Genetic Improvement of Soybean (General, Ministry of Agriculture), Nanjing Agricultural University, Nanjing, Jiangsu, China.
authors.forename: Yufeng
authors.initials: Y
authors.lastname: Wang
authors.affiliation: National Key Laboratory of Crop Genetics and Germplasm Enhancement, National Center for Soybean Improvement, Key Laboratory for Biology and Genetic Improvement of Soybean (General, Ministry of Agriculture), Nanjing Agricultural University, Nanjing, Jiangsu, China.
authors.forename: Tuanjie
authors.initials: T
authors.lastname: Zhao
authors.affiliation: National Key Laboratory of Crop Genetics and Germplasm Enhancement, National Center for Soybean Improvement, Key Laboratory for Biology and Genetic Improvement of Soybean (General, Ministry of Agriculture), Nanjing Agricultural University, Nanjing, Jiangsu, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.affiliation: National Key Laboratory of Crop Genetics and Germplasm Enhancement, National Center for Soybean Improvement, Key Laboratory for Biology and Genetic Improvement of Soybean (General, Ministry of Agriculture), Nanjing Agricultural University, Nanjing, Jiangsu, China.
authors.forename: Junyi
authors.initials: J
authors.lastname: Gai
mesh_terms: D000535:Aluminum / Q000378:metabolism*; D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism*; D003001:Cloning, Molecular; D004789:Enzyme Activation; D018506:Gene Expression Regulation, Plant; D008969:Molecular Sequence Data; D010641:Phenotype; D010802:Phylogeny; D010937:Plant Growth Regulators / Q000494:pharmacology; D030821:Plants, Genetically Modified; D021381:Protein Transport; D055049:Salt Tolerance / Q000235:genetics*; D016415:Sequence Alignment; D013025:Glycine max / Q000235:genetics*; D013312:Stress, Physiological; D044767:Ubiquitin-Protein Ligases / Q000235:genetics*; D054875:Ubiquitination
chemical_list: D010937:Plant Growth Regulators; D000535:Aluminum; D044767:Ubiquitin-Protein Ligases