title: DHT inhibits the Aβ25-35-induced apoptosis by regulation of seladin-1, survivin, XIAP, bax, and bcl-xl expression through a rapid PI3-K/Akt signaling in C6 glial cell lines.
abstract: Previous evidences indicate that androgen is neuroprotective in the brain. However, the underling mechanisms remain to be fully elucidated. Moreover, it is controversial whether dihydrotestosterone (DHT) modulates the expression of apoptosis-related effectors, such as survivin, XIAP, bax, and bcl-xl proteins mediated by the PI3-K/Akt pathway, which contributes to androgen neuroprotection. In this study using a C6 glial cell model, apoptotic cells were detected by flow cytometry. Akt, seladin-1, survivin, XIAP, bcl-xl, and bax protein expression is investigated by Western blot. After amyloid β-protein fragment (Aβ25-35) treatment, apoptotic cells at early (annexin V+, PI-) and late (annexin V+, PI+) stages were significantly increased. Apoptosis at early and late was obviously inhibited in the presence of DHT. The effect of DHT was markedly blocked by PI3-K inhibitor LY294002.To elicit the mechanism of DHT protection, the expression of seladin-1, survivin, XIAP, bax, and bcl-xl protein was determined in C6 cells treated with Aβ25-35, DHT, or LY294002. Aβ25-35 significantly downregulated the expression of seladin-1, survivin, XIAP, bcl-xl protein and upregulated the expression of bax protein. DHT significantly inhibited the expression of bax, seladin-1, survivin, XIAP, and bcl-xl protein induced by Aβ25-35. Further, we found the effect of DHT was significantly inhibited by LY294002. Collectively, in a C6 glial cell model, we firstly found that DHT inhibits Aβ25-35-induced apoptosis by a rapid nongenic PI-3K/Akt activation as well as regulation of seladin-1, survivin, XIAP, bcl-xl, and bax proteins.
authors.affiliation: Department of Neurology, Jinshan Hospital Affiliated to Fudan University, No. 1508 Longhang Road, Jinshan District, Shanghai, 201508, China.
authors.forename: Lelin
authors.initials: L
authors.lastname: Bing
authors.forename: Junfeng
authors.initials: J
authors.lastname: Wu
authors.forename: Jianfeng
authors.initials: J
authors.lastname: Zhang
authors.forename: Yinghui
authors.initials: Y
authors.lastname: Chen
authors.forename: Zhen
authors.initials: Z
authors.lastname: Hong
authors.forename: Hengbing
authors.initials: H
authors.lastname: Zu
mesh_terms: D016229:Amyloid beta-Peptides / Q000037:antagonists & inhibitors* / Q000633:toxicity*; D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002460:Cell Line; D013196:Dihydrotestosterone / Q000494:pharmacology*; D051034:Inhibitor of Apoptosis Proteins / Q000187:drug effects*; D008869:Microtubule-Associated Proteins / Q000378:metabolism*; D009419:Nerve Tissue Proteins / Q000187:drug effects*; D009457:Neuroglia / Q000187:drug effects*; D044925:Oxidoreductases Acting on CH-CH Group Donors / Q000187:drug effects*; D010446:Peptide Fragments / Q000037:antagonists & inhibitors* / Q000633:toxicity*; D019869:Phosphatidylinositol 3-Kinases / Q000187:drug effects*; D051057:Proto-Oncogene Proteins c-akt / Q000187:drug effects*; D051381:Rats; D011944:Receptors, Androgen / Q000187:drug effects; D015398:Signal Transduction / Q000187:drug effects; D000077022:Survivin; D051028:bcl-2-Associated X Protein / Q000187:drug effects*; D051020:bcl-X Protein / Q000187:drug effects*
chemical_list: D016229:Amyloid beta-Peptides; D013196:Dihydrotestosterone; D051034:Inhibitor of Apoptosis Proteins; D008869:Microtubule-Associated Proteins; D009419:Nerve Tissue Proteins; D044925:Oxidoreductases Acting on CH-CH Group Donors; D010446:Peptide Fragments; D019869:Phosphatidylinositol 3-Kinases; D051057:Proto-Oncogene Proteins c-akt; D011944:Receptors, Androgen; D000077022:Survivin; D051028:bcl-2-Associated X Protein; D051020:bcl-X Protein; C494828:Bax protein, rat; C494813:Bcl2l1 protein, rat; C516989:Birc5 protein, rat; C494837:Xiap protein, rat; C074636:amyloid beta-protein (25-35); C000600134:DHCR24 protein, rat