title: Quantitative trait loci pyramiding can improve the nutritional potential of tomato (Solanum lycopersicum) fruits.
abstract: Solanum lycopersicum represents an important source of antioxidants and other bioactive compounds. Previously two Solanum pennellii introgression lines (IL 7-3 and IL 12-4) were identified as carrying quantitative trait loci (QTL) increasing fruit ascorbic acid and phenolics content. Novel tomato lines were obtained by pyramiding these selected QTLs in the genetic background of the cultivated line M82. Pyramided lines revealed significant increases of total phenolics, phenolic acids, ascorbic acid, and total antioxidant activity compared to parental lines IL 7-3 and IL 12-4 and the cultivated line M82. In addition, tomato extracts obtained from the pyramided lines had no cytotoxic effect on normal human cells while exhibiting a selective cytotoxic effect on aggressive cancer cells. Therefore, the present study demonstrates that it is possible to incorporate favorable wild-species QTLs in the cultivated genetic background to obtain genotypes with higher nutritional value.
authors.affiliation: Department of Agricultural Sciences, University of Naples Federico II , Via Università 100, 80055 Portici (Naples), Italy.
authors.forename: Maria Manuela
authors.initials: MM
authors.lastname: Rigano
authors.forename: Assunta
authors.initials: A
authors.lastname: Raiola
authors.forename: Gian Carlo
authors.initials: GC
authors.lastname: Tenore
authors.forename: Daria Maria
authors.initials: DM
authors.lastname: Monti
authors.forename: Rita
authors.initials: R
authors.lastname: Del Giudice
authors.forename: Luigi
authors.initials: L
authors.lastname: Frusciante
authors.forename: Amalia
authors.initials: A
authors.lastname: Barone
mesh_terms: D000975:Antioxidants / Q000032:analysis / Q000494:pharmacology; D001205:Ascorbic Acid / Q000032:analysis / Q000494:pharmacology; D002338:Carotenoids / Q000032:analysis / Q000494:pharmacology; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D005838:Genotype; D006801:Humans; D018551:Solanum lycopersicum / Q000235:genetics*; D009753:Nutritive Value; D010636:Phenols / Q000032:analysis / Q000494:pharmacology; D010936:Plant Extracts / Q000032:analysis / Q000494:pharmacology; D040641:Quantitative Trait Loci
chemical_list: D000975:Antioxidants; D010636:Phenols; D010936:Plant Extracts; D002338:Carotenoids; D001205:Ascorbic Acid
keywords: MTT test; Solanum lycopersicum; antioxidant power; ascorbic acid; phenolic acid; phenolics