title: Application of response surface methodology for rapid chrysene biodegradation by newly isolated marine-derived fungus Cochliobolus lunatus strain CHR4D.
abstract: For the first time, Cochliobolus lunatus strain CHR4D, a marine-derived ascomycete fungus isolated from historically contaminated crude oil polluted shoreline of Alang-Sosiya ship-breaking yard, at Bhavnagar coast, Gujarat has been reported showing the rapid and enhanced biodegradation of chrysene, a four ringed high molecular weight (HMW) polycyclic aromatic hydrocarbon (PAH). Mineral Salt Broth (MSB) components such as ammonium tartrate and glucose along with chrysene, pH and trace metal solution have been successfully optimized by Response Surface Methodology (RSM) using central composite design (CCD). A validated, two-step optimization protocol has yielded a substantial 93.10% chrysene degradation on the 4(th) day, against unoptimized 56.37% degradation on the 14(th) day. The results depict 1.65 fold increase in chrysene degradation and 1.40 fold increase in biomass with a considerable decrement in time. Based on the successful laboratory experiments, C. lunatus strain CHR4D can thus be predicted as a potential candidate for mycoremediation of HMW PAHs impacted environments.
authors.affiliation: Department of Life Sciences, Maharaja Krishnakumarsinhji Bhavnagar University, Sardar Vallabhbhai Patel Campus, Gaurishankar Lake Road, Bhavnagar, Gujarat, 364002, India.
authors.forename: Jwalant K
authors.initials: JK
authors.lastname: Bhatt
authors.forename: Chirag M
authors.initials: CM
authors.lastname: Ghevariya
authors.forename: Dushyant R
authors.initials: DR
authors.lastname: Dudhagara
authors.forename: Rahul K
authors.initials: RK
authors.lastname: Rajpara
authors.forename: Bharti P
authors.initials: BP
authors.lastname: Dave
mesh_terms: D059001:Aquatic Organisms / Q000302:isolation & purification / Q000378:metabolism; D001203:Ascomycota / Q000302:isolation & purification / Q000378:metabolism*; D001673:Biodegradation, Environmental; D002911:Chrysenes / Q000378:metabolism*; D052918:Environmental Restoration and Remediation / Q000379:methods*; D019015:Geologic Sediments / Q000382:microbiology*; D011084:Polycyclic Aromatic Hydrocarbons / Q000378:metabolism
chemical_list: D002911:Chrysenes; D011084:Polycyclic Aromatic Hydrocarbons; C031180:chrysene