title: Quantitative peptidomics study reveals that a wound-induced peptide from PR-1 regulates immune signaling in tomato.
abstract: Many important cell-to-cell communication events in multicellular organisms are mediated by peptides, but only a few peptides have been identified in plants. In an attempt to address the difficulties in identifying plant signaling peptides, we developed a novel peptidomics approach and used this approach to discover defense signaling peptides in plants. In addition to the canonical peptide systemin, several novel peptides were confidently identified in tomato (Solanum lycopersicum) and quantified to be induced by both wounding and methyl jasmonate (MeJA). A wounding or wounding plus MeJA-induced peptide derived from the pathogenesis-related protein 1 (PR-1) family was found to induce significant antipathogen and minor antiherbivore responses in tomato. This study highlights a role for PR-1 in immune signaling and suggests the potential application of plant endogenous peptides in efforts to defeat biological threats in crop production. As PR-1 is highly conserved across many organisms and the putative peptide from At-PR1 was also found to be bioactive in Arabidopsis thaliana, our results suggest that this peptide may be useful for enhancing resistance to stress in other plant species.
authors.affiliation: Agricultural Biotechnology Research Center, Academia Sinica, Taipei 11529, Taiwan Department of Life Science and Institute of Plant Biology, National Taiwan University, Taipei 10617, Taiwan.
authors.forename: Ying-Lan
authors.initials: YL
authors.lastname: Chen
authors.affiliation: Agricultural Biotechnology Research Center, Academia Sinica, Taipei 11529, Taiwan.
authors.forename: Chi-Ying
authors.initials: CY
authors.lastname: Lee
authors.affiliation: Agricultural Biotechnology Research Center, Academia Sinica, Taipei 11529, Taiwan.
authors.forename: Kai-Tan
authors.initials: KT
authors.lastname: Cheng
authors.affiliation: Agricultural Biotechnology Research Center, Academia Sinica, Taipei 11529, Taiwan.
authors.forename: Wei-Hung
authors.initials: WH
authors.lastname: Chang
authors.affiliation: Department of Entomology and Research Center for Plant Medicine, National Taiwan University, Taipei 10617, Taiwan.
authors.forename: Rong-Nan
authors.initials: RN
authors.lastname: Huang
authors.affiliation: Center for Plant Aging Research, Institute for Basic Science, Daegu 711-873, Republic of Korea Department of New Biology, Daegu Gyeongbuk Institute of Science and Technology, Daegu 711-873, Republic of Korea.
authors.forename: Hong Gil
authors.initials: HG
authors.lastname: Nam
authors.affiliation: Agricultural Biotechnology Research Center, Academia Sinica, Taipei 11529, Taiwan Department of Bioscience and Biotechnology, National Taiwan Ocean University, Keelung 20224, Taiwan yetran@gate.sinica.edu.tw.
authors.forename: Yet-Ran
authors.identifier: http://orcid.org/0000-0002-2409-6655
authors.initials: YR
authors.lastname: Chen
mesh_terms: D000085:Acetates / Q000494:pharmacology; D000595:Amino Acid Sequence; D002853:Chromatography, Liquid; D003517:Cyclopentanes / Q000494:pharmacology; D060467:Disease Resistance / Q000187:drug effects / Q000235:genetics / Q000276:immunology; D054884:Host-Pathogen Interactions / Q000187:drug effects / Q000276:immunology; D018551:Solanum lycopersicum / Q000235:genetics / Q000378:metabolism* / Q000382:microbiology; D008969:Molecular Sequence Data; D020411:Oligonucleotide Array Sequence Analysis; D054883:Oxylipins / Q000494:pharmacology; D010455:Peptides / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology; D010935:Plant Diseases / Q000235:genetics / Q000382:microbiology; D010937:Plant Growth Regulators / Q000494:pharmacology; D018515:Plant Leaves / Q000235:genetics / Q000378:metabolism / Q000382:microbiology; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D020543:Proteome / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology; D040901:Proteomics; D044224:Pseudomonas syringae / Q000276:immunology / Q000502:physiology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013314:Stress, Mechanical; D053719:Tandem Mass Spectrometry; D059467:Transcriptome / Q000187:drug effects / Q000235:genetics / Q000276:immunology
chemical_list: D000085:Acetates; D003517:Cyclopentanes; D054883:Oxylipins; D010455:Peptides; D010937:Plant Growth Regulators; D010940:Plant Proteins; D020543:Proteome; C053376:pathogenesis-related proteins, plant; C072239:methyl jasmonate