title: Deficiency of Long Pentraxin PTX3 Promoted Neointimal Hyperplasia after Vascular Injury.
abstract: OBJECTIVE
  Pentraxin 3 (PTX3) is a novel marker for the primary local activation of innate immunity and inflammatory responses. Although clinical and experimental evidence suggests that PTX3 is associated with atherosclerosis, the relationship between PTX3 and vascular remodeling after wall injury remains to be determined. We investigated the effects of PTX3 on neointimal hyperplasia following wire vascular injury.
  
  
  METHODS
  PTX3 systemic knockout (PTX3-KO) mice and wild-type littermate (WT) mice were subjected to wire-mediated endovascular injury. At four weeks after wire-mediated injury, the areas of neointimal and medial hyperplasia were evaluated.
  
  
  RESULTS
  The PTX3-KO mice exhibited higher hyperplasia/media ratios than the WT mice after wire injury, and the degree of Mac-3-positive macrophage accumulation was significantly higher in the PTX3-KO mice than in the WT mice. Furthermore, the PTX3-KO mice showed a much greater increase in the number of PCNA-stained cells in the vascular wall than that observed in the WT mice.
  
  
  CONCLUSIONS
  A deficiency of PTX3 results in deteriorated neointimal hyperplasia after vascular injury via the effects of macrophage accumulation and vascular smooth muscle cell proliferation and migration.
authors.affiliation: Department of Cardiology, Pulmonology, and Nephrology, Yamagata University School of Medicine.
authors.forename: Mitsunori
authors.initials: M
authors.lastname: Ishino
authors.forename: Tetsuro
authors.initials: T
authors.lastname: Shishido
authors.forename: Satoshi
authors.initials: S
authors.lastname: Suzuki
authors.forename: Shigehiko
authors.initials: S
authors.lastname: Katoh
authors.forename: Toshiki
authors.initials: T
authors.lastname: Sasaki
authors.forename: Akira
authors.initials: A
authors.lastname: Funayama
authors.forename: Shunsuke
authors.initials: S
authors.lastname: Netsu
authors.forename: Hiromasa
authors.initials: H
authors.lastname: Hasegawa
authors.forename: Shintaro
authors.initials: S
authors.lastname: Honda
authors.forename: Hiroki
authors.initials: H
authors.lastname: Takahashi
authors.forename: Takanori
authors.initials: T
authors.lastname: Arimoto
authors.forename: Takehiko
authors.initials: T
authors.lastname: Miyashita
authors.forename: Takuya
authors.initials: T
authors.lastname: Miyamoto
authors.forename: Tetsu
authors.initials: T
authors.lastname: Watanabe
authors.forename: Yasuchika
authors.initials: Y
authors.lastname: Takeishi
authors.forename: Isao
authors.initials: I
authors.lastname: Kubota
mesh_terms: D000818:Animals; D002097:C-Reactive Protein / Q000502:physiology*; D002465:Cell Movement; D049109:Cell Proliferation; D006965:Hyperplasia / Q000209:etiology* / Q000378:metabolism / Q000473:pathology; D008264:Macrophages / Q000378:metabolism / Q000473:pathology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009131:Muscle, Smooth, Vascular / Q000378:metabolism / Q000473:pathology*; D058426:Neointima / Q000209:etiology* / Q000378:metabolism / Q000473:pathology; D009419:Nerve Tissue Proteins / Q000502:physiology*; D057772:Vascular System Injuries / Q000150:complications* / Q000378:metabolism / Q000473:pathology; D000099153:Pentraxins
chemical_list: D002097:C-Reactive Protein; D009419:Nerve Tissue Proteins; D000099153:Pentraxins