title: Discovery of catalytic phages by biocatalytic self-assembly.
abstract: Discovery of new catalysts for demanding aqueous reactions is challenging. Here, we describe methodology for selection of catalytic phages by taking advantage of localized assembly of the product of the catalytic reaction that is screened for. A phage display library covering 10(9) unique dodecapeptide sequences is incubated with nonassembling precursors. Phages which are able to catalyze formation of the self-assembling reaction product (via amide condensation) acquire an aggregate of reaction product, enabling separation by centrifugation. The thus selected phages can be amplified by infection of Escherichia coli. These phages are shown to catalyze amide condensation and hydrolysis. Kinetic analysis shows a minor role for substrate binding. The approach enables discovery and mass-production of biocatalytic phages.
authors.affiliation: Department of Chemistry and Biochemistry, Hunter College, City University of New York , 695 Park Avenue, New York, New York 10065, United States.
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Maeda
authors.forename: Nadeem
authors.initials: N
authors.lastname: Javid
authors.forename: Krystyna
authors.initials: K
authors.lastname: Duncan
authors.forename: Louise
authors.initials: L
authors.lastname: Birchall
authors.forename: Kirsty F
authors.initials: KF
authors.lastname: Gibson
authors.forename: Daniel
authors.initials: D
authors.lastname: Cannon
authors.forename: Yuka
authors.initials: Y
authors.lastname: Kanetsuki
authors.forename: Charles
authors.initials: C
authors.lastname: Knapp
authors.forename: Tell
authors.initials: T
authors.lastname: Tuttle
authors.forename: Rein V
authors.initials: RV
authors.lastname: Ulijn
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Matsui
mesh_terms: D000577:Amides / Q000737:chemistry; D000595:Amino Acid Sequence; D017104:Bacteriophage M13 / Q000378:metabolism* / Q000502:physiology; D055162:Biocatalysis; D004926:Escherichia coli / Q000821:virology; D006868:Hydrolysis; D009842:Oligopeptides / Q000737:chemistry
chemical_list: D000577:Amides; D009842:Oligopeptides