title: Polymorphism, genetic effect and association with egg production traits of chicken matrix metalloproteinases 9 promoter.
abstract: Matrix metalloproteinases (MMP) are key enzymes involved in cell and tissue remodeling during ovarian follicle development and ovulation. The control of MMP9 transcription in ovarian follicles occurs through a core promoter region (-2,400 to -1,700 bp). The aim of this study was to screen genetic variations in the core promoter region and examine MMP9 transcription regulation and reproduction performance. A single cytosine deletion/insertion polymorphism was found at -1954 C(+)/C(-). Genetic association analysis indicated significant correlation between the deletion genotype (C(-)) with total egg numbers at 28 weeks (p = 0.031). Furthermore, luciferase-reporter assay showed the deletion genotype (C(-)) had significantly lower promoter activity than the insertion genotype (C(+)) in primary granulosa cells (p<0.01). Therefore, the identified polymorphism could be used for marker-assisted selection to improve chicken laying performance.
authors.affiliation: Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Taian 271018, China ; Department of Biology Science and Technology, Taishan University, Taian 271021, China .
authors.forename: Guiyu
authors.initials: G
authors.lastname: Zhu
authors.affiliation: Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Taian 271018, China.
authors.forename: Yunliang
authors.initials: Y
authors.lastname: Jiang
keywords: Chicken; Matrix Metalloproteinases 9; Ovary; Polymorphism; Reproduction