title: Multiple NUCLEAR FACTOR Y transcription factors respond to abiotic stress in Brassica napus L.
abstract: Members of the plant NUCLEAR FACTOR Y (NF-Y) family are composed of the NF-YA, NF-YB, and NF-YC subunits. In Brassica napus (canola), each of these subunits forms a multimember subfamily. Plant NF-Ys were reported to be involved in several abiotic stresses. In this study, we demonstrated that multiple members of thirty three BnNF-Ys responded rapidly to salinity, drought, or ABA treatments. Transcripts of five BnNF-YAs, seven BnNF-YBs, and two BnNF-YCs were up-regulated by salinity stress, whereas the expression of thirteen BnNF-YAs, ten BnNF-YBs, and four BnNF-YCs were induced by drought stress. Under NaCl treatments, the expression of one BnNF-YA10 and four NF-YBs (BnNF-YB3, BnNF-YB7, BnNF-YB10, and BnNF-YB14) were greatly increased. Under PEG treatments, the expression levels of four NF-YAs (BnNF-YA9, BnNF-YA10, BnNF-YA11, and BnNF-YA12) and five NF-YBs (BnNF-YB1, BnNF-YB8, BnNF-YB10, BnNF-YB13, and BnNF-YB14) were greatly induced. The expression profiles of 20 of the 27 salinity- or drought-induced BnNF-Ys were also affected by ABA treatment. The expression levels of six NF-YAs (BnNF-YA1, BnNF-YA7, BnNF-YA8, BnNF-YA9, BnNF-YA10, and BnNF-YA12) and seven BnNF-YB members (BnNF-YB2, BnNF-YB3, BnNF-YB7, BnNF-YB10, BnNF-YB11, BnNF-YB13, and BnNF-YB14) and two NF-YC members (BnNF-YC2 and BnNF-YC3) were greatly up-regulated by ABA treatments. Only a few BnNF-Ys were inhibited by the above three treatments. Several NF-Y subfamily members exhibited collinear expression patterns. The promoters of all stress-responsive BnNF-Ys harbored at least two types of stress-related cis-elements, such as ABRE, DRE, MYB, or MYC. The cis-element organization of BnNF-Ys was similar to that of Arabidopsis thaliana, and the promoter regions exhibited higher levels of nucleotide sequence identity with Brassica rapa than with Brassica oleracea. This work represents an entry point for investigating the roles of canola NF-Y proteins during abiotic stress responses and provides insight into the genetic evolution of Brassica NF-Ys.
authors.affiliation: College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing, China.
authors.forename: Li
authors.initials: L
authors.lastname: Xu
authors.affiliation: College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing, China; Jiangsu Key Lab of Marine Biology, Nanjing, China.
authors.forename: Zhongyuan
authors.initials: Z
authors.lastname: Lin
authors.affiliation: College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing, China; Jiangsu Key Lab of Marine Biology, Nanjing, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Tao
authors.affiliation: College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing, China; Jiangsu Key Lab of Marine Biology, Nanjing, China.
authors.forename: Mingxiang
authors.initials: M
authors.lastname: Liang
authors.affiliation: College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing, China; Jiangsu Key Lab of Marine Biology, Nanjing, China.
authors.forename: Gengmao
authors.initials: G
authors.lastname: Zhao
authors.affiliation: College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing, China; Jiangsu Key Lab of Marine Biology, Nanjing, China.
authors.forename: Xiangzhen
authors.initials: X
authors.lastname: Yin
authors.affiliation: College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing, China; Jiangsu Key Lab of Marine Biology, Nanjing, China.
authors.forename: Ruixin
authors.initials: R
authors.lastname: Fu
mesh_terms: D000040:Abscisic Acid / Q000494:pharmacology; D029681:Arabidopsis Proteins / Q000235:genetics; D001483:Base Sequence; D029688:Brassica napus / Q000187:drug effects / Q000235:genetics / Q000502:physiology*; D023081:CCAAT-Binding Factor / Q000235:genetics / Q000378:metabolism*; D055864:Droughts; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D017343:Genes, Plant; D010802:Phylogeny; D018515:Plant Leaves / Q000187:drug effects / Q000235:genetics; D018517:Plant Roots / Q000187:drug effects / Q000235:genetics; D020218:Response Elements / Q000235:genetics; D054712:Salinity; D012689:Sequence Homology, Nucleic Acid; D013312:Stress, Physiological / Q000187:drug effects / Q000235:genetics
chemical_list: D029681:Arabidopsis Proteins; D023081:CCAAT-Binding Factor; C402244:nuclear factor Y; D000040:Abscisic Acid