title: Electrophoretic mobility shift assays: analysis of tRNA binding to the T box riboswitch antiterminator RNA.
abstract: Changes in electrophoretic mobility upon complex formation with RNA can be used to probe structure-function relationships that are critical for complex formation. Here, we describe the application of this technique to monitor tRNA binding to the T box riboswitch antiterminator RNA.
authors.affiliation: Department of Chemistry and Biochemistry, Ohio University, Clippinger Laboratory, 1 Ohio Universitys, Athens, OH, 45701, USA.
authors.forename: R
authors.initials: R
authors.lastname: Anupam
authors.forename: S
authors.initials: S
authors.lastname: Zhou
authors.forename: J V
authors.initials: JV
authors.lastname: Hines
mesh_terms: D001345:Autoradiography; D001412:Bacillus subtilis / Q000378:metabolism; D001483:Base Sequence; D024202:Electrophoretic Mobility Shift Assay / Q000379:methods*; D008969:Molecular Sequence Data; D012329:RNA, Bacterial / Q000235:genetics / Q000378:metabolism*; D012343:RNA, Transfer / Q000235:genetics / Q000378:metabolism*; D058928:Riboswitch; D012996:Solutions; D013194:Staining and Labeling; D013728:Terminator Regions, Genetic
chemical_list: D012329:RNA, Bacterial; D058928:Riboswitch; D012996:Solutions; D012343:RNA, Transfer