title: Seabuckthron (Hippophae rhamnoides L.) leaf extract ameliorates the gamma radiation mediated DNA damage and hepatic alterations.
abstract: In vitro assessment showed that H. rhamnoides (HrLE) extract possessed free radical scavenging activities and can protect gamma (gamma) radiation induced supercoiled DNA damage. For in vivo study, Swiss albino mice were administered with HrLE (30 mg/kg body weight) for 15 consecutive days before exposing them to a single dose of 5 Gy of beta radiation. HrLE significantly prevented the radiation induced genomic DNA damage indicated as a significant reduction in the comet parameters. The lipid peroxidation, liver function enzymes, expression of phosphorylated NFkappaB (p65) and IkappaBalpha increased whereas the endogenous antioxidants diminished upon radiation exposure compared to control. Pretreatment of HrLE extract ameliorated these changes. Based on the present results it can be concluded that H. rhamnoides possess a potential preventive element in planned and accidental nuclear exposures.
authors.forename: Amitava
authors.initials: A
authors.lastname: Khan
authors.forename: Krishnendu
authors.initials: K
authors.lastname: Manna
authors.lastname: Chinchubose
authors.forename: Dipesh Kr
authors.initials: DK
authors.lastname: Das
authors.forename: Mahuya
authors.initials: M
authors.lastname: Sinha
authors.forename: Swaraj Bandhu
authors.initials: SB
authors.lastname: Kesh
authors.forename: Ujjal
authors.initials: U
authors.lastname: Das
authors.forename: Rakhi Sharma
authors.initials: RS
authors.lastname: Dey
authors.forename: Asoke
authors.initials: A
authors.lastname: Banerji
authors.forename: Sanjit
authors.initials: S
authors.lastname: Dey
mesh_terms: D000818:Animals; D004249:DNA Damage / Q000187:drug effects*; D004278:DNA, Superhelical / Q000737:chemistry / Q000187:drug effects / Q000528:radiation effects; D016166:Free Radical Scavengers / Q000737:chemistry / Q000494:pharmacology*; D005720:Gamma Rays; D031281:Hippophae / Q000737:chemistry*; D008099:Liver / Q000737:chemistry / Q000187:drug effects* / Q000473:pathology; D008297:Male; D051379:Mice; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry
chemical_list: D004278:DNA, Superhelical; D016166:Free Radical Scavengers; D010936:Plant Extracts