title: Canine distemper virus envelope protein interactions modulated by hydrophobic residues in the fusion protein globular head.
abstract: Membrane fusion for morbillivirus cell entry relies on critical interactions between the viral fusion (F) and attachment (H) envelope glycoproteins. Through extensive mutagenesis of an F cavity recently proposed to contribute to F's interaction with the H protein, we identified two neighboring hydrophobic residues responsible for severe F-to-H binding and fusion-triggering deficiencies when they were mutated in combination. Since both residues reside on one side of the F cavity, the data suggest that H binds the F globular head domain sideways.
authors.affiliation: Division of Neurological Sciences, DCR-VPH, Vetsuisse Faculty, University of Bern, Bern, Switzerland Graduate School for Cellular and Biomedical Sciences, University of Bern, Bern, Switzerland.
authors.forename: Mislay
authors.initials: M
authors.lastname: Avila
authors.affiliation: Division of Neurological Sciences, DCR-VPH, Vetsuisse Faculty, University of Bern, Bern, Switzerland Graduate School for Cellular and Biomedical Sciences, University of Bern, Bern, Switzerland.
authors.forename: Mojtaba
authors.initials: M
authors.lastname: Khosravi
authors.affiliation: Division of Neurological Sciences, DCR-VPH, Vetsuisse Faculty, University of Bern, Bern, Switzerland Graduate School for Cellular and Biomedical Sciences, University of Bern, Bern, Switzerland.
authors.forename: Lisa
authors.initials: L
authors.lastname: Alves
authors.affiliation: Division of Neurological Sciences, DCR-VPH, Vetsuisse Faculty, University of Bern, Bern, Switzerland Graduate School for Cellular and Biomedical Sciences, University of Bern, Bern, Switzerland.
authors.forename: Nadine
authors.initials: N
authors.lastname: Ader-Ebert
authors.affiliation: Division of Neurological Sciences, DCR-VPH, Vetsuisse Faculty, University of Bern, Bern, Switzerland Graduate School for Cellular and Biomedical Sciences, University of Bern, Bern, Switzerland.
authors.forename: Fanny
authors.initials: F
authors.lastname: Bringolf
authors.affiliation: Division of Neurological Sciences, DCR-VPH, Vetsuisse Faculty, University of Bern, Bern, Switzerland.
authors.forename: Andreas
authors.initials: A
authors.lastname: Zurbriggen
authors.affiliation: Institute for Biomedical Sciences, Georgia State University, Atlanta, Georgia, USA.
authors.forename: Richard K
authors.initials: RK
authors.lastname: Plemper
authors.affiliation: Division of Neurological Sciences, DCR-VPH, Vetsuisse Faculty, University of Bern, Bern, Switzerland philippe.plattet@vetsuisse.unibe.ch.
authors.forename: Philippe
authors.initials: P
authors.lastname: Plattet
mesh_terms: D000818:Animals; D004252:DNA Mutational Analysis; D004217:Distemper Virus, Canine / Q000235:genetics / Q000502:physiology*; D008958:Models, Molecular; D011485:Protein Binding; D011487:Protein Conformation; D055503:Protein Multimerization; D014759:Viral Envelope Proteins / Q000235:genetics / Q000378:metabolism*; D053586:Virus Internalization
chemical_list: D014759:Viral Envelope Proteins