title: Purification and characterization of a novel laccase from Cerrena sp. HYB07 with dye decolorizing ability.
abstract: Laccases (EC 1.10.3.2) are a class of multi-copper oxidases with important industrial values. A basidiomycete strain Cerrena sp. HYB07 with high laccase yield was identified. After cultivation in the shaking flask for 4 days, a maximal activity of 210.8 U mL(-1) was attained. A 58.6-kDa laccase (LacA) with 7.2% carbohydrate and a specific activity of 1952.4 U mg(-1) was purified. 2,2'-Azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) was the optimal substrate, with Km and kcat being 93.4 µM and 2468.0 s(-1), respectively. LacA was stable at 60°C, pH 5.0 and above, and in organic solvents. Metal ions Na+, K+, Ca2+, Mg2+, Mn2+, Zn2+ enhanced LacA activity, while Fe2+ and Li+ inhibited LacA activity. LacA decolorized structurally different dyes and a real textile effluent. Its gene and cDNA sequences were obtained. Putative cis-acting transcriptional response elements were identified in the promoter region. The high production yield and activity, robustness and dye decolorizing capacity make LacA and Cerrena sp. HYB07 potentially useful for industrial and environmental applications such as textile finishing and wastewater treatment.
authors.affiliation: College of Biological Sciences and Technology, Fuzhou University, Fuzhou, Fujian, China; National Engineering Laboratory for Enzyme Expression, Fuzhou, Fujian, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Yang
authors.affiliation: College of Biological Sciences and Technology, Fuzhou University, Fuzhou, Fujian, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Lin
authors.affiliation: School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China.
authors.forename: Tzi Bun
authors.initials: TB
authors.lastname: Ng
authors.affiliation: College of Biological Sciences and Technology, Fuzhou University, Fuzhou, Fujian, China; National Engineering Laboratory for Enzyme Expression, Fuzhou, Fujian, China.
authors.forename: Xiuyun
authors.initials: X
authors.lastname: Ye
authors.affiliation: College of Biological Sciences and Technology, Fuzhou University, Fuzhou, Fujian, China; National Engineering Laboratory for Enzyme Expression, Fuzhou, Fujian, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Lin
mesh_terms: D001487:Basidiomycota / Q000201:enzymology*; D004396:Coloring Agents / Q000737:chemistry*; D005656:Fungal Proteins / Q000737:chemistry* / Q000302:isolation & purification*; D042845:Laccase / Q000737:chemistry* / Q000302:isolation & purification*; D013783:Textile Industry; D018508:Water Purification
chemical_list: D004396:Coloring Agents; D005656:Fungal Proteins; D042845:Laccase