title: Genetic association analyses of fast plasma glucose level in pre-menopausal Chinese women: potential interaction between osteocalcin and oestrogen receptor α.
abstract: BACKGROUND
  Fasting plasma glucose (FPG) levels are usually tightly regulated within a narrow physiologic range. Variation of FPG levels is clinically important and is strongly heritable. Several lines of evidence suggest the importance of the oestrogen receptor α (ER-α) and osteocalcin (also known as BGP, for bone Gla protein) in determining FPG; however, whether their polymorphisms are associated with FPG variation is not well understood.
  
  
  OBJECTIVE
  To investigate whether ER-a PvuII and BGP HindIII genetic polymorphisms and their potential interaction are associated with FPG variation.
  
  
  METHODS
  The study subjects were 328 unrelated pre-menopausal Chinese women aged 21 years and over (mean age ± SD, 33.2 ± 5.9 years), with an average FPG of 4.92 (SD = 0.81). All subjects were genotyped at the ER-α PvuII and BGP HindIII loci using polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP).
  
  
  RESULTS
  The ER-α PvuII genotypes were significantly associated with FPG (p = 0.007). In addition, a significant interaction was observed of the ER-α PvuII polymorphism with BGP HindIII polymorphism on FPG variation (p = 0.013), although the BGP HindIII polymorphism was not shown to be individually associated with FPG.
  
  
  CONCLUSIONS
  The PvuII polymorphism of the ER-α gene and its potential interaction with the HindIII polymorphism of the BGP gene were associated with FPG in pre-menopausal Chinese women.
authors.affiliation: a Department of Physiology , Jiangxi Medical College of Nanchang University , Nanchang , Jiangxi , PR China .
authors.forename: Hong
authors.initials: H
authors.lastname: Xu
authors.affiliation: a Department of Physiology , Jiangxi Medical College of Nanchang University , Nanchang , Jiangxi , PR China .
authors.forename: Lu-Ling
authors.initials: LL
authors.lastname: He
authors.affiliation: b Foreign Nursing Class-2013 Grade, Fuzhou Medical College of Nanchang University , Fuzhou , Jiangxi , PR China .
authors.forename: Chao-Peng
authors.initials: CP
authors.lastname: Xiong
authors.affiliation: c School of Life Science & Technology, China Pharmaceutical University , Nanjing , PR China .
authors.forename: Cheng-Xin
authors.initials: CX
authors.lastname: Gong
authors.affiliation: a Department of Physiology , Jiangxi Medical College of Nanchang University , Nanchang , Jiangxi , PR China .
authors.forename: Chang-Le
authors.initials: CL
authors.lastname: Liu
authors.affiliation: d Experimental Class-2011 Grade .
authors.forename: Lu-Lu
authors.initials: LL
authors.lastname: Peng
authors.affiliation: d Experimental Class-2011 Grade .
authors.forename: Ya-Jun
authors.initials: YJ
authors.lastname: Cheng
authors.affiliation: e Analysis Class-2011 Grade , and.
authors.forename: Fu-Qing
authors.initials: FQ
authors.lastname: Jiang
authors.affiliation: e Analysis Class-2011 Grade , and.
authors.forename: Li-Ping
authors.initials: LP
authors.lastname: Tan
authors.affiliation: f Clinic 6 Class-2012 Grade, Jiangxi Medical College of Nanchang University , Nanchang , Jiangxi , PR China .
authors.forename: Lan
authors.initials: L
authors.lastname: Tang
authors.affiliation: d Experimental Class-2011 Grade .
authors.forename: Wei
authors.initials: W
authors.lastname: Peng
authors.affiliation: g Department of Endocrinology , The Fourth Affiliated Hospital of Nanchang University , Nanchang , Jiangxi , PR China , and.
authors.forename: Yun-Ming
authors.initials: YM
authors.lastname: Tu
authors.affiliation: g Department of Endocrinology , The Fourth Affiliated Hospital of Nanchang University , Nanchang , Jiangxi , PR China , and.
authors.forename: Yu-Ping
authors.initials: YP
authors.lastname: Yang
authors.affiliation: a Department of Physiology , Jiangxi Medical College of Nanchang University , Nanchang , Jiangxi , PR China .
authors.forename: Dan
authors.initials: D
authors.lastname: Luo
authors.affiliation: h Department of Radioimmunology , People's Hospital of Jiangxi Province , Nanchang , Jiangxi , PR China.
authors.forename: Lin
authors.initials: L
authors.lastname: Zou
authors.affiliation: a Department of Physiology , Jiangxi Medical College of Nanchang University , Nanchang , Jiangxi , PR China .
authors.forename: Shang-Dong
authors.initials: SD
authors.lastname: Liang
mesh_terms: D000328:Adult; D044466:Asian People; D001786:Blood Glucose / Q000502:physiology*; D002681:China; D047628:Estrogen Receptor alpha / Q000235:genetics / Q000378:metabolism*; D005260:Female; D056726:Genetic Association Studies; D006801:Humans; D015675:Osteocalcin / Q000235:genetics / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length / Q000235:genetics; D017697:Premenopause / Q000502:physiology*; D055815:Young Adult
chemical_list: D001786:Blood Glucose; C506487:ESR1 protein, human; D047628:Estrogen Receptor alpha; D015675:Osteocalcin
keywords: Analysis of covariance (ANCOVA); fasting plasma glucose (FPG); interaction; oestrogen receptor-α (ER-α) gene; osteocalcin (BGP) gene