title: Prognostic value of LAMP3 and TP53 overexpression in benign and malignant gastrointestinal tissues.
abstract: Lysosomal associated membrane protein 3 (LAMP3) is a newly identified tumor-specific protein. It is a downstream target gene of tumor suppressor TP53 and its expression has been associated with hypoxia-induced metastasis and poor overall survival in cervical and breast cancers. However, little is known of LAMP3 protein expression in gastrointestinal cancer and its prognostic value. We determined protein expression of LAMP3 and TP53 in both gastric (n=750) and colorectal (n=479) tissues by immunohistochemistry analysis on tissue microarray (TMA), their expression was correlated with patients' clinical parameters. LAMP3 and TP53 protein expression was significantly higher in cancerous tissues compared to normal and benign tissues. In both gastric and colorectal cancers, high LAMP3 protein expression (LAMP3+) was significantly associated with tumor stage (P=0.014 and P<0.001). No correlation between LAMP3 and TP53 expression was observed. Patients with high LAMP3 expression but not high TP53 expression had a poor overall survival (for gastric cancer P<0.001, CI: 1.762-4.567; for colorectal cancer P=0.036, CI: 1.062-5.980). Our data suggest that epithelial LAMP3 expression is an independent prognostic marker for gastrointestinal cancer.
authors.affiliation: Department of Pathology, Nantong University Affiliated Hospital, Nantong, Jiangsu, China. Department of General Surgery, Nantong University Affiliated Hospital, Nantong, Jiangsu, China.
authors.forename: Rongwei
authors.initials: R
authors.lastname: Sun
authors.affiliation: Department of Laboratory Medicine, Nantong University Affiliated Hospital, Nantong, Jiangsu, China.
authors.forename: Xudong
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Pathology, Nantong University Affiliated Hospital, Nantong, Jiangsu, China.
authors.forename: Huijun
authors.initials: H
authors.lastname: Zhu
authors.affiliation: Department of General Surgery, Nantong University Affiliated Hospital, Nantong, Jiangsu, China.
authors.forename: Haijun
authors.initials: H
authors.lastname: Mei
authors.affiliation: Department of Pathology, Nantong University Affiliated Hospital, Nantong, Jiangsu, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.affiliation: Department of Pathology, Nantong University Affiliated Hospital, Nantong, Jiangsu, China.
authors.forename: Shu
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Department of Pathology, Nantong University Affiliated Hospital, Nantong, Jiangsu, China.
authors.forename: Jianfei
authors.initials: J
authors.lastname: Huang
mesh_terms: D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000032:analysis*; D005260:Female; D005770:Gastrointestinal Neoplasms / Q000378:metabolism / Q000401:mortality / Q000473:pathology*; D006801:Humans; D007150:Immunohistochemistry; D053208:Kaplan-Meier Estimate; D051907:Lysosomal Membrane Proteins / Q000096:biosynthesis*; D008297:Male; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000096:biosynthesis*; D011379:Prognosis; D016016:Proportional Hazards Models; D046888:Tissue Array Analysis; D016159:Tumor Suppressor Protein p53 / Q000096:biosynthesis*; D059666:Lysosomal-Associated Membrane Protein 3
chemical_list: D014408:Biomarkers, Tumor; D051907:Lysosomal Membrane Proteins; D009363:Neoplasm Proteins; D016159:Tumor Suppressor Protein p53; C114233:LAMP3 protein, human; D059666:Lysosomal-Associated Membrane Protein 3; C495901:TP53 protein, human