title: N-(2-amino-5-chlorobenzoyl)benzamidoxime derivatives inhibit human leukemia cell growth.
abstract: OBJECTIVE
  Amidoxime derivatives have been previously reported to have potent anti-microbial and anti-tumor activity. Little is known about the tumor cell growth-inhibition mechanism of amidoximes, especially benzamidoxime derivatives. Herein we determined the effects of N-(2-amino-5-chlorobenzoyl)benzamidoxime analogs on mammalian cancer cells.
  
  
  METHODS
  We synthesized four chloride-substituted benzamidoxime analogs from the original benzamidoxime to investigate their anticancer cell activity using the Jurkat T-cell lymphoma cell line and the human leukemia cell line HL-60RG.
  
  
  RESULTS
  All amidoxime derivatives inhibited Jurkat and HL-60RG cell viability dose-dependently. Benzamidoximes tended to damage HL-60RG cells to a greater extent compared to Jurkat cells. Benzamidoximes with chloride substitutes caused a strong decrease in cell growth, and this cell growth attenuation was transient at 5 μM (below the half-maximal inhibitory concentration, IC50) but long-lasting at 10 μM (greater than the IC50).
  
  
  CONCLUSIONS
  Benzamidoxime derivatives caused a transient cell-cycle delay at a low dose and cell death at a high dose.
authors.affiliation: Division of Life Science and Engineering, College of Science and Engineering, Tokyo Denki University, Hatoyama, Hiki-gun, Saitama, Japan yuki@mail.dendai.ac.jp.
authors.forename: Yukitoshi
authors.initials: Y
authors.lastname: Nagahara
authors.affiliation: Department of Pharmaceutical Life Sciences, Faculty of Pharmacy, Kitasato University, 5-9-1 Shirokane, Minato-ku, Tokyo, Japan.
authors.forename: Katsuhiko
authors.initials: K
authors.lastname: Nagahara
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D001550:Benzamidines / Q000737:chemistry*; D002453:Cell Cycle / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects*; D005434:Flow Cytometry; D006131:Growth Inhibitors / Q000494:pharmacology*; D006801:Humans; D007938:Leukemia / Q000188:drug therapy* / Q000473:pathology*; D016399:Lymphoma, T-Cell / Q000188:drug therapy* / Q000473:pathology*; D015394:Molecular Structure; D014407:Tumor Cells, Cultured
chemical_list: D001550:Benzamidines; D006131:Growth Inhibitors; C053561:benzamidoxime
keywords: Benzamidoxime; HL-60RG; Jurkat; apoptosis; cancer