title: Effect of Euphorbia cyparissias ointments on acanthosis.
abstract: BACKGROUND
  A pharmaco-chemical investigation of the Euphorbia cyparissias plant was justified by its known multiple therapeutic valences. Numerous components from extracts and latex of Euphorbiacae were identified, revealing a large plant family with a polyvalent therapeutic activity.
  
  
  METHODS
  The aim of the study was to assess the skin tolerance level to irritation on different testing concentrations, of Euphorbia cyparissias extracts and ointments. Study was accomplished in rats and dogs, with the identification of all possible skin injuries and histological changes, after a simple patch test methodology.
  
  
  RESULTS
  Ointment dermatological testing on rats, proved to be bearable on epilated skin at concentrations of 1, 2 and 5%. Ointments and mother tincture with higher concentrations (10% and 20%), led to irritation and cutis damages, and this was revealed through histology.
  
  
  CONCLUSIONS
  Ointment tested on dog's skin was tolerable for epilated skin to concentrations of 1, 2 and/or 5%, additional testing on human volunteers confirmed the same situation.
authors.affiliation: Pharmacology and Pharmacy Dept., USAMVB, Faculty of Veterinary Medicine, 119, Calea Aradului, Timişoara, Romania.
authors.forename: Romeo T
authors.initials: RT
authors.lastname: Cristina
authors.affiliation: Pharmacology and Pharmacy Dept., USAMVB, Faculty of Veterinary Medicine, 119, Calea Aradului, Timişoara, Romania.
authors.forename: Eugenia
authors.initials: E
authors.lastname: Dumitrescu
authors.affiliation: Histology and Molecular biology Dept., USAMVB, Faculty of Veterinary Medicine, 119, Calea Aradului, Timişoara, Romania.
authors.forename: Diana
authors.initials: D
authors.lastname: Brezovan
authors.affiliation: Botany Dept., USAMVB, Faculty of Veterinary Medicine, 119, Calea Aradului, Timişoara, Romania.
authors.forename: Florin
authors.initials: F
authors.lastname: Muselin
authors.affiliation: Drugs Production Dept., Romvac Company, Şos. Centurii no. 7, Voluntari, Romania.
authors.forename: Viorica
authors.initials: V
authors.lastname: Chiurciu
mesh_terms: D000052:Acanthosis Nigricans / Q000188:drug therapy*; D000818:Animals; D004285:Dogs; D028481:Euphorbia / Q000737:chemistry*; D006801:Humans; D009824:Ointments / Q000008:administration & dosage; D010936:Plant Extracts / Q000008:administration & dosage*; D051381:Rats
chemical_list: D009824:Ointments; D010936:Plant Extracts
keywords: Acanthosis; Euphorbia cyparissias; dermatology test; dog; ointments; rat