title: Effects of histamine on ciliary beat frequency of ciliated cells from guinea pigs nasal mucosa.
abstract: We aimed to investigate the effect of histamine on ciliary beat frequency (CBF) through combining high-speed digital microscopy and patch-clamp technology. Ciliated cells were obtained from septum and turbinate of 90-120-day-old healthy male guinea pigs. Tight seal was formed by applying negative pressure on the glass electrode after the drawing and pushing progress. Then, we enrolled high-speed digital microscopy to measure CBF before and after treatment with histamine of different concentrations ranging from 10(-6) to 10(-1) mol/L in Hank's solution and D-Hank's solution as well as after administrating adenosine triphosphate. One-way ANOVA, Student's t test or Kruskal-Wallis test was used for statistical comparisons. Glass electrode fix up ciliated cell is available at tip diameter of 2-5 μm and negative pressure of 10-20 cmH2O column. The baseline CBF in Hank's solution was higher than in D-Hank's solution. Treatment with 10(-6)-l0(-3) mol/L histamine of concentrations can stimulate a rise of CBF. Nevertheless, CBF in all groups decreased to baseline CBF within 20 min. Generally, 10(-2) mol/L histamine can stimulate a rise of CBF; meanwhile, the high concentration of histamine killed 50% ciliated cell. Histamine at 10(-1) mol/L killed all ciliated cells. Ciliary beating activity decreased in Ca(2+)-free solution. Moreover, adenosine triphosphate could increase CBF effectively after the stimulation effect of histamine. We construct an effective technology integrating patch-clamp technique with CBF measurements on ciliated cells. Extracellular histamine stimulation could increase CBF effectively.
authors.affiliation: Department of Otorhinolaryngology, Jinan Military General Hospital, Jinan, 250031, Shandong Province, China.
authors.forename: Fengwei
authors.initials: F
authors.lastname: An
authors.forename: Lijun
authors.initials: L
authors.lastname: Xing
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Zhang
authors.forename: Lei
authors.initials: L
authors.lastname: Chen
mesh_terms: D000818:Animals; D002923:Cilia / Q000187:drug effects* / Q000502:physiology; D006168:Guinea Pigs; D006632:Histamine / Q000008:administration & dosage*; D017442:Histamine Agonists / Q000008:administration & dosage; D008297:Male; D009297:Nasal Mucosa / Q000166:cytology / Q000187:drug effects*; D018408:Patch-Clamp Techniques; D012996:Solutions
chemical_list: D017442:Histamine Agonists; D012996:Solutions; D006632:Histamine