title: Over-expression of SlSHN1 gene improves drought tolerance by increasing cuticular wax accumulation in tomato.
abstract: Increasing cuticular wax accumulation in plants has been associated with improving drought tolerance in plants. In this study, a cDNA clone encoding the SlSHN1 transcription factor, the closest ortholog to WIN/SHN1 gene in Arabidopsis, was isolated from tomato plant. Expression analysis of SlSHN1 indicated that it is induced in response to drought conditions. The over-expression of SlSHN1 in tomato under the control of the constitutive CaMV 35S promoter produced plants that showed mild growth retardation phenotype with shiny and dark green leaves. Scanning electron microscopy showed that the over-expression of SlSHN1 in tomato resulted in higher cuticular wax deposition on leaf epidermial tissue when compared to non-transformed plants. Expression analysis in transgenic lines over-expressing SlSHN1 indicated that several wax-related synthesis genes were induced. Transgenic tomato plants over-expressing SlSHN1 showed higher drought tolerance when compared with wild type plants; this was reflected in delayed wilting of transgenic lines, improved water status and reduced water loss rate when compared with wild type plants. In conclusion, the SlSHN1 gene can modulate wax accumulation and could be utilized to enhance drought tolerance in tomato plant.
authors.affiliation: Department of Horticulture and Crop Science, Faculty of Agriculture, the University of Jordan, Amman 11942, Jordan. a.alabdallat@ju.edu.jo.
authors.forename: Ayed M
authors.initials: AM
authors.lastname: Al-Abdallat
authors.affiliation: Department of Horticulture and Crop Science, Faculty of Agriculture, the University of Jordan, Amman 11942, Jordan. debeih@ju.edu.jo.
authors.forename: Hmoud S
authors.initials: HS
authors.lastname: Al-Debei
authors.affiliation: Department of Horticulture and Crop Science, Faculty of Agriculture, the University of Jordan, Amman 11942, Jordan. ayadj@ju.edu.jo.
authors.forename: Jamal Y
authors.initials: JY
authors.lastname: Ayad
authors.affiliation: Hamdi Mango Center for Scientific Research, the University of Jordan, Amman 11942, Jordan. s.hasan@ju.edu.jo.
authors.forename: Shireen
authors.initials: S
authors.lastname: Hasan
mesh_terms: D000222:Adaptation, Physiological / Q000235:genetics*; D000595:Amino Acid Sequence; D055864:Droughts; D015870:Gene Expression; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D018551:Solanum lycopersicum / Q000235:genetics* / Q000378:metabolism*; D008969:Molecular Sequence Data; D010641:Phenotype; D010802:Phylogeny; D018515:Plant Leaves / Q000378:metabolism / Q000648:ultrastructure; D030821:Plants, Genetically Modified; D016415:Sequence Alignment; D014885:Waxes / Q000378:metabolism*
chemical_list: D014885:Waxes