title: New strategy to apply perfluorodecalin as an oxygen carrier in lipase production: minimisation and reuse.
abstract: A novel strategy for the production of lipase by Bacillus sp. ITP-001 in a stirred tank fermenter using perfluorodecalin (PFD) was studied. Firstly, a response surface methodology 2(2) with three central points was employed to optimise the effect of agitation speed and aeration rate in lipase production. According to the response from the experimental designs, 300 rpm (revolutions per minute) and 0.5 vvm (air volume/liquid volume per minute) were found to provide the best condition (lipolytic activity: LA = 3,140.76 U mL(-1)). Then, the influence of PFD concentration on the fermentation process was evaluated. Incorporation of PFD at all concentrations above 1% had no statistically significant influence on lipase production, that is, the previous optimisation allowed the reduction of the amount of PFD added besides increasing lipase production. Furthermore, PFD could be used in three sequential fermentations without altering the statistical production of lipase, reducing by 67% the cost of PFD addition.
authors.affiliation: Programa de Pós-Graduação em Engenharia de Processos, Universidade Tiradentes, Av. Murilo Dantas 300, Farolândia, Aracaju, 49032-490, Brazil.
authors.forename: Erika Souza
authors.initials: ES
authors.lastname: Vieira
authors.forename: Tâmara Karoline
authors.initials: TK
authors.lastname: de Oliveira Fontes
authors.forename: Matheus Mendonça
authors.initials: MM
authors.lastname: Pereira
authors.forename: Hofsky Vieira
authors.initials: HV
authors.lastname: Alexandre
authors.forename: Daniel Pereira
authors.initials: DP
authors.lastname: da Silva
authors.forename: Cleide Mara Faria
authors.initials: CM
authors.lastname: Soares
authors.forename: Álvaro Silva
authors.initials: ÁS
authors.lastname: Lima
mesh_terms: D001407:Bacillus / Q000378:metabolism*; D018533:Biomass; D019149:Bioreactors; D005285:Fermentation; D005466:Fluorocarbons / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D007218:Industrial Microbiology; D008049:Lipase / Q000096:biosynthesis*; D010100:Oxygen / Q000737:chemistry*; D013696:Temperature
chemical_list: D005466:Fluorocarbons; C008805:perfluorodecalin; D008049:Lipase; D010100:Oxygen