title: Suppressive effect of interleukin 10 on priming of naive hepatitis C virus-specific CD8+ T cells.
abstract: Growing evidence suggests a role for the immunomodulatory cytokine interleukin-10 (IL-10) in hepatitis C virus (HCV)-specific CD8(+) T-cell failure. To address the possible role of IL-10 during priming, we performed in vitro priming experiments with naive HCV-specific CD8(+) T cells and autologous monocyte-derived dendritic cells in the absence or presence of IL-10. Our results showed that IL-10, when present during priming, significantly reduced the frequency of HCV-specific CD8(+) T cells after coculture; It was directly targeting CD8(+) T cells and led to impaired effector cell differentiation. These results may provide a possible mechanistic basis for the association between early IL-10 elevation, T-cell failure, and viral persistence.
authors.affiliation: Department of Medicine II, University Hospital Freiburg.
authors.forename: Emanuel
authors.initials: E
authors.lastname: Niesen
authors.affiliation: Department of Medicine II, University Hospital Freiburg.
authors.forename: Julia
authors.initials: J
authors.lastname: Schmidt
authors.affiliation: Department of Medicine II, University Hospital Freiburg Spemann Graduate School of Biology and Medicine Faculty of Biology, Albert-Ludwigs University Freiburg, Germany.
authors.forename: Tobias
authors.initials: T
authors.lastname: Flecken
authors.affiliation: Department of Medicine II, University Hospital Freiburg.
authors.forename: Robert
authors.initials: R
authors.lastname: Thimme
mesh_terms: D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D002478:Cells, Cultured; D018920:Coculture Techniques; D003713:Dendritic Cells / Q000276:immunology*; D016174:Hepacivirus / Q000276:immunology*; D006801:Humans; D016753:Interleukin-10 / Q000276:immunology*
chemical_list: D016753:Interleukin-10
keywords: CD8+ T cells; CD8+ T-cell responses; HCV; IL-10; chronic hepatitis C; chronic viral infection; dendritic cells; primary activation