title: Controversial effect on Erk activation of some cytotoxic drugs in human LOVO colon cancer cells.
abstract: The use of some classic antibiotics was recently shown to inhibit growth and to induce apoptosis in human LOVO colon cancer cells. In this study, we describe that ciprofloxacin (CI), trimebutine maleate (COL) and tiemonium methylsulfate (VIS) greatly inhibit cell proliferation in vitro. Proliferation inhibition reached its maximum at 10(-4 )M, 10(-3 )M and 10(-2 )M, respectively, for COL, CI and VIS. Moreover, phospho-extracellular-regulated kinase was totally abrogated in non-apoptotic cytotoxicity of VIS but decreases or increases in the apoptotic inhibition, respectively, of COL and CI treatments.
  
  
  BACKGROUND
  CI: ciprofloxacin; COL: trimebutine maleate; VIS: tiemonium methylsulfate; MAPK/Erk: mitogen-activated protein kinases/extracellular-regulated kinase.
authors.affiliation: a Center of Biotechnology of Sfax , Sfax , Tunisia and.
authors.forename: Ikram
authors.initials: I
authors.lastname: Jemel-Oualha
authors.affiliation: a Center of Biotechnology of Sfax , Sfax , Tunisia and.
authors.forename: Jihene
authors.initials: J
authors.lastname: Elloumi-Mseddi
authors.affiliation: a Center of Biotechnology of Sfax , Sfax , Tunisia and.|b Institute of Molecular Immunology, Klinikum rechts der Isar, Technische Universität , Munich , Germany.
authors.forename: Abdelhamid
authors.initials: A
authors.lastname: Beji
authors.affiliation: a Center of Biotechnology of Sfax , Sfax , Tunisia and.
authors.forename: Bochra
authors.initials: B
authors.lastname: Hakim
authors.affiliation: a Center of Biotechnology of Sfax , Sfax , Tunisia and.
authors.forename: Sami
authors.initials: S
authors.lastname: Aifa
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D015153:Blotting, Western; D049109:Cell Proliferation / Q000187:drug effects; D002939:Ciprofloxacin / Q000494:pharmacology*; D003110:Colonic Neoplasms / Q000188:drug therapy / Q000378:metabolism* / Q000473:pathology*; D004789:Enzyme Activation; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism*; D005765:Gastrointestinal Agents / Q000494:pharmacology; D006801:Humans; D009025:Morpholines / Q000494:pharmacology*; D059005:Topoisomerase II Inhibitors / Q000494:pharmacology; D014287:Trimebutine / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
chemical_list: D005765:Gastrointestinal Agents; D009025:Morpholines; D059005:Topoisomerase II Inhibitors; D002939:Ciprofloxacin; C020526:tiemonium methylsulfate; D048049:Extracellular Signal-Regulated MAP Kinases; D014287:Trimebutine
keywords: Colorectal cancer; Erk activation; ciprofloxacin; proliferation; tiemonium methylsulfate; trimebutin maleate