title: Bmi-1 induces radioresistance by suppressing senescence in human U87 glioma cells.
abstract: Radiotherapy is the main locoregional control modality for a number of types of malignant tumors, including glioblastoma. However, radiotherapy fails to prevent recurrence in numerous patients due to the intrinsic radioresistance of cancer cells. Cell senescence is significant in tumor suppressor mechanisms and is closely associated with the radioresistance of cancer cells. Bmi-1 has been proposed to be an oncogene that can induce anti-senescence in tumor cells. The present study investigated the response of U87 glioma cells to radiation exposure and the role of Bmi-1 in the response following radiotherapy. Cell apoptosis and cell cycle distribution were assessed using flow cytometry, and a SA-β-Gal stain was used to observe the senescence ratio of U87 cells following radiation. The expression of Bmi-1 in U87 cells exposed to different doses of radiation was evaluated by western blot analysis. X-ray radiation was found to inhibit U87 cell proliferation through the induction of senescence rather than apoptosis. Following exposure to radiation, the cell cycle distribution was dysregulated, with an increased number of cells in the G2/M phase, and the expression of Bmi-1 was upregulated, particularly when a dose of ≥6 Gy was administered. The results indicated that senescence is the main mechanism by which U87 cell growth is inhibited following radiation. In addition, Bmi-1 may be significant in increasing the radioresistance of glioma cells by enabling cell senescence.
authors.affiliation: Cancer Center, The Second Hospital of Shandong University, Jinan, Shandong 250033, P.R. China.
authors.forename: Lan
authors.initials: L
authors.lastname: Ye
authors.affiliation: Cancer Center, The Second Hospital of Shandong University, Jinan, Shandong 250033, P.R. China.
authors.forename: Cuihong
authors.initials: C
authors.lastname: Wang
authors.affiliation: Jinan Central Hospital, Jinan, Shandong 250014, P.R. China.
authors.forename: Guanying
authors.initials: G
authors.lastname: Yu
authors.affiliation: Cancer Center, The Second Hospital of Shandong University, Jinan, Shandong 250033, P.R. China.
authors.forename: Yuhua
authors.initials: Y
authors.lastname: Jiang
authors.affiliation: Cancer Center, The Second Hospital of Shandong University, Jinan, Shandong 250033, P.R. China.
authors.forename: Dianshui
authors.initials: D
authors.lastname: Sun
authors.affiliation: Cancer Center, The Second Hospital of Shandong University, Jinan, Shandong 250033, P.R. China.
authors.forename: Zaiyun
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Cancer Center, The Second Hospital of Shandong University, Jinan, Shandong 250033, P.R. China.
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Yu
authors.affiliation: Cancer Center, The Second Hospital of Shandong University, Jinan, Shandong 250033, P.R. China.
authors.forename: Xiaomei
authors.initials: X
authors.lastname: Li
authors.affiliation: Cancer Center, The Second Hospital of Shandong University, Jinan, Shandong 250033, P.R. China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wei
authors.affiliation: Cancer Center, The Second Hospital of Shandong University, Jinan, Shandong 250033, P.R. China.
authors.forename: Ping
authors.initials: P
authors.lastname: Liu
authors.affiliation: Cancer Center, The Second Hospital of Shandong University, Jinan, Shandong 250033, P.R. China.
authors.forename: Jian
authors.initials: J
authors.lastname: Cheng
authors.affiliation: Jinan Central Hospital, Jinan, Shandong 250014, P.R. China.
authors.forename: Bin
authors.initials: B
authors.lastname: DU
authors.affiliation: Department of Radiotherapy, Qilu Hospital, Shandong University, Jinan, Shandong 250012, P.R. China.
authors.forename: Likuan
authors.initials: L
authors.lastname: Hu
keywords: Bmi-1; glioma; radiation; senescence