title: Garlic-derived compound S-allylmercaptocysteine inhibits cell growth and induces apoptosis via the JNK and p38 pathways in human colorectal carcinoma cells.
abstract: S-allylmercaptocysteine (SAMC) is an active compound that is derived from garlic and has been demonstrated to possess antitumor propertiesin vitro. The present study aimed to investigate the effect of SAMC and determine the underlying mechanism of this effect on human colorectal carcinoma cells. The SW620 cells were cultured with various concentrations of SAMC and cell viability was detected using an MTT assay. Analysis of apoptosis was performed using terminal deoxynucleotidyl-transferase-mediated deoxyuridine triphosphate nick end labeling. The c-Jun N-terminal kinase (JNK) and p38 mitogen activated protein kinase (p38) signaling pathways were investigated by polymerase chain reaction. SAMC was observed to reduce cell viability in a dose- and time-dependent manner, partially through the induction of apoptosis in human colorectal carcinoma cells. At the molecular level, SAMC induces apoptosis through JNK and p38 signaling pathways, increasing tumor protein p53 (p53) and Bax activation in the SW620 cells. The most effective concentration of SAMC for the induction of SW620 cell apoptosis was found to be 400 μM, which was confirmed through cell viability assays and apoptosis analysis. The current study indicated that SAMC inhibits cell proliferation and induces apoptosis of SW620 cells via the JNK and p38 pathways. The results from the current study demonstrated that SAMC must be further investigated as a novel preventive or therapeutic agent for the treatment of colorectal carcinoma, and potentially for use in other tumor types.
authors.affiliation: Department of Colorectal Surgery, Third Hospital of Hebei Medical University, Shijiazhuang, Hebei 050051, P.R. China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Colorectal Surgery, Third Hospital of Hebei Medical University, Shijiazhuang, Hebei 050051, P.R. China.
authors.forename: Hong-Yan
authors.initials: HY
authors.lastname: Li
authors.affiliation: Department of English, Hebei University of Science and Technology, Shijiazhuang, Hebei 050051, P.R. China.
authors.forename: Zhi-Hua
authors.initials: ZH
authors.lastname: Zhang
authors.affiliation: Department of Colorectal Surgery, Third Hospital of Hebei Medical University, Shijiazhuang, Hebei 050051, P.R. China.
authors.forename: Hong-Lei
authors.initials: HL
authors.lastname: Bian
authors.affiliation: Department of Colorectal Surgery, Third Hospital of Hebei Medical University, Shijiazhuang, Hebei 050051, P.R. China.
authors.forename: Gui
authors.initials: G
authors.lastname: Lin
keywords: S-allylmercaptocysteine; SW620; c-Jun N-terminal kinase; human colorectal carcinoma cells; p38; p53; signal pathway