title: Effects of hemostatic agents on fibroblast cells.
abstract: OBJECTIVE
  Hemostatic agents may be used topically to control hemorrhage, especially in patients with bleeding disorders. The agent used may have a negative effect on the tissue prolonging the healing time. The aim of this study was to compare the effects of 3 different hemostatic agents on fibroblast cells on a rat primary fibroblast cell culture model.
  
  
  METHODS
  Ankaferd Blood Stopper (ABD) (Ankaferd Pharmaceuticals Cosmetics Production and Marketing Co.), fibrin glue, and tranexamic acid were the agents to be evaluated for their effects on cell proliferation, cell numbers, cell viability, and cell morphology. Also lactate dehydrogenase, basic fibroblast growth factor, and vascular endothelial growth factor C levels were measured.
  
  
  RESULTS
  It was found that all of the agents used in the study have negative effects on fibroblasts, with ABD having the lowest values of cell proliferation, cell number, and cell viability.
  
  
  CONCLUSIONS
  The results of this study indicate that ABD, fibrin glue, and tranexamic acid may negatively affect tissue healing.
authors.affiliation: *Associate Professor, Faculty of Dentistry, Department of Oral and Maxillofacial Surgery, Istanbul University, Istanbul, Turkey. †Professor, Faculty of Dentistry, Department of Oral and Maxillofacial Surgery, Istanbul University, Istanbul, Turkey. ‡Professor, Istanbul Faculty of Medicine, Department of Biochemistry, Istanbul University, Istanbul, Turkey. §Professor, Istanbul Faculty of Medicine, Department of Public Health, Istanbul University, Istanbul, Turkey. ‖Professor, Faculty of Dentistry, Department of Oral and Maxillofacial Surgery, Istanbul University, Istanbul, Turkey. ¶Associate Professor, Istanbul University, Faculty of Dentistry, Department of Oral and Maxillofacial Surgery, Istanbul, Turkey. #Research Assistant, Istanbul University, Faculty of Dentistry, Department of Endodontics Istanbul, Turkey. **Professor, Istanbul University, Istanbul Faculty of Medicine, Department of Histology and Embryology, Istanbul, Turkey.
authors.forename: Yusuf
authors.initials: Y
authors.lastname: Emes
authors.forename: Buket
authors.initials: B
authors.lastname: Aybar
authors.forename: Pervin
authors.initials: P
authors.lastname: Vural
authors.forename: Halim
authors.initials: H
authors.lastname: Işsever
authors.forename: Serhat
authors.initials: S
authors.lastname: Yalçn
authors.forename: Belir
authors.initials: B
authors.lastname: Atalay
authors.forename: Emir
authors.initials: E
authors.lastname: Dinçol
authors.forename: Ayhan
authors.initials: A
authors.lastname: Bilir
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000032:analysis; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D015718:Fibrin Tissue Adhesive / Q000494:pharmacology; D016222:Fibroblast Growth Factor 2 / Q000032:analysis; D005347:Fibroblasts / Q000187:drug effects*; D006490:Hemostatics / Q000494:pharmacology*; D007770:L-Lactate Dehydrogenase / Q000032:analysis; D008855:Microscopy, Electron, Scanning; D010936:Plant Extracts / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014148:Tranexamic Acid / Q000494:pharmacology; D042461:Vascular Endothelial Growth Factor A / Q000032:analysis
chemical_list: D015415:Biomarkers; D015718:Fibrin Tissue Adhesive; D006490:Hemostatics; D010936:Plant Extracts; D042461:Vascular Endothelial Growth Factor A; C539751:ankaferd blood stopper; D016222:Fibroblast Growth Factor 2; D014148:Tranexamic Acid; D007770:L-Lactate Dehydrogenase