title: Full-length genome sequence analysis of a Hungarian porcine reproductive and respiratory syndrome virus isolated from a pig with severe respiratory disease.
abstract: Here, we report the isolation of a type 1 porcine reproductive and respiratory syndrome virus (PRRSV) strain from a clinical outbreak of severe respiratory problems and high fever. Next-generation sequencing was used to determine the complete genome sequence of the isolate (9625/2012). The virus belongs to a new branch within subtype 1, clade D, and shows the highest similarity to PRRSV Olot/1991 and to the Amervac vaccine strain. Mutation analysis of 9625/2012 revealed no evidence of recombination but did show a high proportion of amino acid substitutions in the putative neutralizing epitopes, suggesting an important role of selective immune pressure in the evolution of PRRSV 9625/2012.
authors.affiliation: National Food Chain Safety Office Veterinary Diagnostic Directorate, Tábornok u. 2, 1149, Budapest, Hungary, balintad@nebih.gov.hu.
authors.forename: Ádám
authors.initials: Á
authors.lastname: Bálint
authors.forename: Gyula
authors.initials: G
authors.lastname: Balka
authors.forename: Péter
authors.initials: P
authors.lastname: Horváth
authors.forename: Sándor
authors.initials: S
authors.lastname: Kecskeméti
authors.forename: Ádám
authors.initials: Á
authors.lastname: Dán
authors.forename: Attila
authors.initials: A
authors.lastname: Farsang
authors.forename: Levente
authors.initials: L
authors.lastname: Szeredi
authors.forename: Krisztián
authors.initials: K
authors.lastname: Bányai
authors.forename: Dániel
authors.initials: D
authors.lastname: Bartha
authors.forename: Ferenc
authors.initials: F
authors.lastname: Olasz
authors.forename: Sándor
authors.initials: S
authors.lastname: Belák
authors.forename: Zoltán
authors.initials: Z
authors.lastname: Zádori
mesh_terms: D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D000818:Animals; D001483:Base Sequence; D060085:Coinfection; D004196:Disease Outbreaks / Q000662:veterinary*; D016679:Genome, Viral / Q000235:genetics*; D059014:High-Throughput Nucleotide Sequencing; D006814:Hungary / Q000453:epidemiology; D008969:Molecular Sequence Data; D009154:Mutation; D045705:Mycoplasma hyopneumoniae; D045729:Pneumonia of Swine, Mycoplasmal / Q000473:pathology / Q000821:virology; D019318:Porcine Reproductive and Respiratory Syndrome / Q000453:epidemiology* / Q000473:pathology / Q000821:virology; D019316:Porcine respiratory and reproductive syndrome virus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D012367:RNA, Viral / Q000235:genetics; D016415:Sequence Alignment; D017423:Sequence Analysis, RNA / Q000662:veterinary; D013552:Swine
chemical_list: D012367:RNA, Viral