title: Quantitative assessment of graded burn wounds in a porcine model using spatial frequency domain imaging (SFDI) and laser speckle imaging (LSI).
abstract: Accurate and timely assessment of burn wound severity is a critical component of wound management and has implications related to course of treatment. While most superficial burns and full thickness burns are easily diagnosed through visual inspection, burns that fall between these extremes are challenging to classify based on clinical appearance. Because of this, appropriate burn management may be delayed, increasing the risk of scarring and infection. Here we present an investigation that employs spatial frequency domain imaging (SFDI) and laser speckle imaging (LSI) as non-invasive technologies to characterize in-vivo burn severity. We used SFDI and LSI to investigate controlled burn wounds of graded severity in a Yorkshire pig model. Burn wounds were imaged starting at one hour after the initial injury and daily at approximately 24, 48 and 72 hours post burn. Biopsies were taken on each day in order to correlate the imaging data to the extent of burn damage as indicated via histological analysis. Changes in reduced scattering coefficient and blood flow could be used to categorize burn severity as soon as one hour after the burn injury. The results of this study suggest that SFDI and LSI information have the potential to provide useful metrics for quantifying the extent and severity of burn injuries.
authors.affiliation: Beckman Laser Institute and Medical Clinic, University of California, Irvine, 1002 Health Sciences Road East, Irvine, CA 92617, USA ; co-first authors.
authors.forename: Adrien
authors.initials: A
authors.lastname: Ponticorvo
authors.affiliation: United States Army Institute of Surgical Research, 3698 Chambers Pass, Fort Sam Houston, TX, 78234, USA ; co-first authors.
authors.forename: David M
authors.initials: DM
authors.lastname: Burmeister
authors.affiliation: Beckman Laser Institute and Medical Clinic, University of California, Irvine, 1002 Health Sciences Road East, Irvine, CA 92617, USA.
authors.forename: Bruce
authors.initials: B
authors.lastname: Yang
authors.affiliation: Beckman Laser Institute and Medical Clinic, University of California, Irvine, 1002 Health Sciences Road East, Irvine, CA 92617, USA ; Department of Biomedical Engineering, University of California, Irvine, 3120 Natural Sciences II, Irvine, CA 92697, USA.
authors.forename: Bernard
authors.initials: B
authors.lastname: Choi
authors.affiliation: United States Army Institute of Surgical Research, 3698 Chambers Pass, Fort Sam Houston, TX, 78234, USA.
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Christy
authors.affiliation: Beckman Laser Institute and Medical Clinic, University of California, Irvine, 1002 Health Sciences Road East, Irvine, CA 92617, USA.
authors.forename: Anthony J
authors.initials: AJ
authors.lastname: Durkin
keywords: (110.4234) Multispectral and hyperspectral imaging; (170.3660) Light propagation in tissues; (170.3880) Medical and biological imaging; (170.6510) Spectroscopy, tissue diagnostics