title: Bio-mimetic nanostructure self-assembled from Au@Ag heterogeneous nanorods and phage fusion proteins for targeted tumor optical detection and photothermal therapy.
abstract: Nanomaterials with near-infrared (NIR) absorption have been widely studied in cancer detection and photothermal therapy (PTT), while it remains a great challenge in targeting tumor efficiently with minimal side effects. Herein we report a novel multifunctional phage-mimetic nanostructure, which was prepared by layer-by-layer self-assembly of Au@Ag heterogenous nanorods (NRs) with rhodamine 6G, and specific pVIII fusion proteins. Au@Ag NRs, first being applied for PTT, exhibited excellent stability, cost-effectivity, biocompatibility and tunable NIR absorption. The fusion proteins were isolated from phage DDAGNRQP specifically selected from f8/8 landscape phage library against colorectal cancer cells in a high-throughput way. Considering the definite charge distribution and low molecular weight, phage fusion proteins were assembled on the negatively charged NR core by electrostatic interactions, exposing the N-terminus fused with DDAGNRQP peptide on the surface. The fluorescent images showed that assembled phage fusion proteins can direct the nanostructure into cancer cells. The nanostructure was more efficient than gold nanorods and silver nanotriangle-based photothermal agents and was capable of specifically ablating SW620 cells after 10 min illumination with an 808 nm laser in the light intensity of 4 W/cm(2). The prepared nanostructure would become an ideal reagent for simutaneously targeted optical imaging and PTT of tumor.
authors.affiliation: Laboratory for Biosensing, Qingdao Institute of Bioenergy &Bioprocess Technology, and Key Laboratory of Biofuels, Chinese Academy of Sciences, 189 Songling Road, Qingdao 266101, China, and University of Chinese Academy of Sciences, 19A Yuquan Road, Beijing 100049, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Wang
authors.affiliation: Laboratory for Biosensing, Qingdao Institute of Bioenergy &Bioprocess Technology, and Key Laboratory of Biofuels, Chinese Academy of Sciences, 189 Songling Road, Qingdao 266101, China, and University of Chinese Academy of Sciences, 19A Yuquan Road, Beijing 100049, China.
authors.forename: Pei
authors.initials: P
authors.lastname: Liu
authors.affiliation: Key Laboratory of Chemical Sensing &Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Sun
authors.affiliation: Key Laboratory of Chemical Sensing &Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, China.
authors.forename: Cuncheng
authors.initials: C
authors.lastname: Li
authors.affiliation: Department of Pathobiology, Auburn University, 269 Greene Hall, Auburn, Alabama 36849-5519, United States.
authors.forename: Valery A
authors.initials: VA
authors.lastname: Petrenko
authors.affiliation: Laboratory for Biosensing, Qingdao Institute of Bioenergy &Bioprocess Technology, and Key Laboratory of Biofuels, Chinese Academy of Sciences, 189 Songling Road, Qingdao 266101, China, and University of Chinese Academy of Sciences, 19A Yuquan Road, Beijing 100049, China.
authors.forename: Aihua
authors.initials: A
authors.lastname: Liu
mesh_terms: D032701:Biomimetics; D036022:Capsid Proteins / Q000737:chemistry* / Q000627:therapeutic use; D045744:Cell Line, Tumor; D015179:Colorectal Neoplasms / Q000628:therapy* / Q000648:ultrastructure / Q000821:virology; D006046:Gold / Q000737:chemistry; D006801:Humans; D049329:Nanostructures / Q000737:chemistry* / Q000627:therapeutic use; D043942:Nanotubes / Q000737:chemistry; D010789:Phototherapy; D012235:Rhodamines / Q000737:chemistry / Q000627:therapeutic use; D012834:Silver / Q000737:chemistry; D019265:Spectroscopy, Near-Infrared
chemical_list: D036022:Capsid Proteins; D012235:Rhodamines; C119217:coat protein pVIII, Enterobacteria phage fd; C026188:rhodamine 6G; D012834:Silver; D006046:Gold