title: Study on the refractive index matching effect of ultrasound on optical clearing of bio-tissues based on the derivative total reflection method.
abstract: In recent years, the tissue optical clearing (OC) technique in the biomedicine field has drawn lots of attention. Various physical and chemical methods have been introduced to improve the efficacy of OC. In this study, the effect of the combination of glycerol and ultrasound treatment on OC of in vitro porcine muscle tissues has been investigated. The refractive index (RI) matching mechanism of OC was directly observed based on the derivative total reflection method. A theoretical model was used to simulate the proportion of tissue fluid in the illuminated area. Moreover, the total transmittance spectra have been obtained by a spectrometer over the range from 450 nm to 700 nm. The administration of glycerol and ultrasound has led to an increase of the RI of background medium and a more RI matching environment was achieved. The experimental results support the validity of the ultrasound treatment for OC. The RI matching mechanism has been firstly quantitatively analyzed based on the derivative total reflection method.
authors.affiliation: Key Laboratory of Weak-Light Nonlinear Photonics, Ministry of Education, School of physics and TEDA Applied Physics School, Nankai University, Tianjin 300071, China.
authors.forename: Huanhuan
authors.initials: H
authors.lastname: Zeng
authors.affiliation: Key Laboratory of Weak-Light Nonlinear Photonics, Ministry of Education, School of physics and TEDA Applied Physics School, Nankai University, Tianjin 300071, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Wang
authors.affiliation: Key Laboratory of Weak-Light Nonlinear Photonics, Ministry of Education, School of physics and TEDA Applied Physics School, Nankai University, Tianjin 300071, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Ye
authors.affiliation: Key Laboratory of Weak-Light Nonlinear Photonics, Ministry of Education, School of physics and TEDA Applied Physics School, Nankai University, Tianjin 300071, China.
authors.forename: Zhichao
authors.initials: Z
authors.lastname: Deng
authors.affiliation: Advanced Technology Institute, Nankai University, Tianjin 300071, China.
authors.forename: Jianchun
authors.initials: J
authors.lastname: Mei
authors.affiliation: Key Laboratory of Weak-Light Nonlinear Photonics, Ministry of Education, School of physics and TEDA Applied Physics School, Nankai University, Tianjin 300071, China.
authors.forename: Wenyuan
authors.initials: W
authors.lastname: Zhou
authors.affiliation: Key Laboratory of Weak-Light Nonlinear Photonics, Ministry of Education, School of physics and TEDA Applied Physics School, Nankai University, Tianjin 300071, China.
authors.forename: Chunping
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Weak-Light Nonlinear Photonics, Ministry of Education, School of physics and TEDA Applied Physics School, Nankai University, Tianjin 300071, China ; jjtian@nankai.edu.cn.
authors.forename: Jianguo
authors.initials: J
authors.lastname: Tian
keywords: (170.0170) Medical optics and biotechnology; (170.3660) Light propagation in tissues; (170.6930) Tissue; (170.7170) Ultrasound