title: Synthesis and antibacterial activities of antibacterial peptides with a spiropyran fluorescence probe.
abstract: In this report, antibacterial peptides 1-3 were prepared with a spiropyran fluorescence probe. The probe exhibits a change in fluorescence when isomerized from a colorless spiro-form (spiropyran, Sp) to a colored open-form (merocyanine, Mc) under different chemical environments, which can be used to study the mechanism of antimicrobial activity. Peptides 1-3 exhibit a marked decrease in antimicrobial activity with increasing alkyl chain length. This is likely due to the Sp-Mc isomers in different polar environments forming different aggregate sizes in TBS, as demonstrated by time-dependent dynamic light scattering (DLS). Moreover, peptides 1-3 exhibited low cytotoxicity and hemolytic activity. These probe-modified peptides may provide a novel approach to study the effect of structural changes on antibacterial activity, thus facilitating the design of new antimicrobial agents to combat bacterial infection.
authors.affiliation: Key Lab for Advanced Materials and Institute of Fine Chemicals, East China University of Science and Technology, Shanghai 200237 (P. R. China).
authors.forename: Lei
authors.initials: L
authors.lastname: Chen
authors.affiliation: Key Lab for Advanced Materials and Institute of Fine Chemicals, East China University of Science and Technology, Shanghai 200237 (P. R. China).
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: Key Lab for Advanced Materials and Institute of Fine Chemicals, East China University of Science and Technology, Shanghai 200237 (P. R. China).
authors.forename: Danling
authors.initials: D
authors.lastname: Yang
authors.affiliation: Key Lab for Advanced Materials and Institute of Fine Chemicals, East China University of Science and Technology, Shanghai 200237 (P. R. China).
authors.forename: Rongfeng
authors.initials: R
authors.lastname: Zou
authors.affiliation: Key Lab for Advanced Materials and Institute of Fine Chemicals, East China University of Science and Technology, Shanghai 200237 (P. R. China).
authors.forename: Junchen
authors.initials: J
authors.lastname: Wu
authors.affiliation: Key Lab for Advanced Materials and Institute of Fine Chemicals, East China University of Science and Technology, Shanghai 200237 (P. R. China).
authors.forename: He
authors.initials: H
authors.lastname: Tian
mesh_terms: D000900:Anti-Bacterial Agents / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology*; D023181:Antimicrobial Cationic Peptides / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology*; D001578:Benzopyrans / Q000737:chemistry; D002460:Cell Line; D005456:Fluorescent Dyes / Q000737:chemistry; D006090:Gram-Negative Bacteria / Q000187:drug effects; D006094:Gram-Positive Bacteria / Q000187:drug effects; D006801:Humans; D007211:Indoles / Q000737:chemistry; D020128:Inhibitory Concentration 50; D008826:Microbial Sensitivity Tests; D015394:Molecular Structure; D009574:Nitro Compounds / Q000737:chemistry
chemical_list: D000900:Anti-Bacterial Agents; D023181:Antimicrobial Cationic Peptides; D001578:Benzopyrans; D005456:Fluorescent Dyes; D007211:Indoles; D009574:Nitro Compounds; C088184:spiropyran