title: Development of a surface plasmon resonance biosensing approach for the rapid detection of porcine circovirus type2 in sample solutions.
abstract: A sensitive and label-free analytical approach for the detection of porcine circovirus type 2 (PCV2) instead of PCV2 antibody in serum sample was systematically investigated in this research based on surface plasmon resonance (SPR) with an establishment of special molecular identification membrane. The experimental device for constructing the biosensing analyzer is composed of an integrated biosensor, a home-made microfluidic module, and an electrical control circuit incorporated with a photoelectric converter. In order to detect the PCV2 using the surface plasmon resonance immunoassay, the mercaptopropionic acid has been used to bind the Au film in advance through the known form of the strong S-Au covalent bonds formed by the chemical radical of the mercaptopropionic acid and the Au film. PCV2 antibodies were bonded with the mercaptopropionic acid by covalent -CO-NH- amide bonding. For the purpose of evaluating the performance of this approach, the known concentrations of PCV2 Cap protein of 10 µg/mL, 7.5 µg/mL, 5 µg/mL, 2.5 µg/mL, 1 µg/mL, and 0.5 µg/mL were prepared by diluting with PBS successively and then the delta response units (ΔRUs) were measured individually. Using the data collected from the linear CCD array, the ΔRUs gave a linear response over a wide concentration range of standard known concentrations of PCV2 Cap protein with the R-Squared value of 0.99625. The theoretical limit of detection was calculated to be 0.04 µg/mL for the surface plasmon resonance biosensing approach. Correspondingly, the recovery rate ranged from 81.0% to 89.3% was obtained. In contrast to the PCV2 detection kits, this surface plasmon resonance biosensing system was validated through linearity, precision and recovery, which demonstrated that the surface plasmon resonance immunoassay is reliable and robust. It was concluded that the detection method which is associated with biomembrane properties is expected to contribute much to determine the PCV2 in sample solutions instead of PCV2 antibody in serum samples quantitatively.
authors.affiliation: Department of Electrical Engineering, Henan Agricultural University, Zhengzhou, China; State key laboratory of wheat and maize crop science, Zhengzhou, China.
authors.forename: Jiandong
authors.initials: J
authors.lastname: Hu
authors.affiliation: Department of Electrical Engineering, Henan Agricultural University, Zhengzhou, China.
authors.forename: Tingting
authors.initials: T
authors.lastname: Wang
authors.affiliation: Department of Electrical Engineering, Henan Agricultural University, Zhengzhou, China.
authors.forename: Shun
authors.initials: S
authors.lastname: Wang
authors.affiliation: Zhengzhou Oriole Electronic (Group) Joint-Stock Co., Ltd, Zhengzhou, China.
authors.forename: Mingwen
authors.initials: M
authors.lastname: Chen
authors.affiliation: Department of Electrical Engineering, Henan Agricultural University, Zhengzhou, China.
authors.forename: Manping
authors.initials: M
authors.lastname: Wang
authors.affiliation: Department of Electrical Engineering, Henan Agricultural University, Zhengzhou, China.
authors.forename: Linying
authors.initials: L
authors.lastname: Mu
authors.affiliation: College of Animal Husbandry and Veterinary, Henan Agricultural University, Zhengzhou, China.
authors.forename: Hongyin
authors.initials: H
authors.lastname: Chen
authors.affiliation: School of Human Nutrition and Dietetics, McGill University, Ste Anne de Bellevue, Quebec, Canada.
authors.forename: Xinran
authors.initials: X
authors.lastname: Hu
authors.affiliation: Department of Electronic and Telecommunications, University of Gavle, Gävle, Sweden.
authors.forename: Hao
authors.initials: H
authors.lastname: Liang
authors.affiliation: Department of Electrical Engineering, Henan Agricultural University, Zhengzhou, China.
authors.forename: Juanhua
authors.initials: J
authors.lastname: Zhu
authors.affiliation: College of life sciences, Henan Agricultural University, Zhengzhou, China.
authors.forename: Min
authors.initials: M
authors.lastname: Jiang
mesh_terms: D000818:Animals; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D036022:Capsid Proteins / Q000737:chemistry*; D017925:Circovirus / Q000737:chemistry / Q000302:isolation & purification*; D044085:Microfluidics / Q000295:instrumentation / Q000379:methods; D020349:Surface Plasmon Resonance / Q000379:methods*; D013552:Swine
chemical_list: D036022:Capsid Proteins