title: Extensive pericentric rearrangements in the bread wheat (Triticum aestivum L.) genotype "Chinese Spring" revealed from chromosome shotgun sequence data.
abstract: The bread wheat (Triticum aestivum L.) genotype "Chinese Spring" ("CS") is the reference base in wheat genetics and genomics. Pericentric rearrangements in this genotype were systematically assessed by analyzing homoeoloci for a set of nonredundant genes from Brachypodium distachyon, Triticum urartu, and Aegilops tauschii in the CS chromosome shotgun sequence obtained from individual chromosome arms flow-sorted from CS aneuploid lines. Based on patterns of their homoeologous arm locations, 551 genes indicated the presence of pericentric inversions in at least 10 of the 21 chromosomes. Available data from deletion bin-mapped expressed sequence tags and genetic mapping in wheat indicated that all inversions had breakpoints in the low-recombinant gene-poor pericentromeric regions. The large number of putative intrachromosomal rearrangements suggests the presence of extensive structural differences among the three subgenomes, at least some of which likely occurred during the production of the aneuploid lines of this hexaploid wheat genotype. These differences could have significant implications in wheat genome research where comparative approaches are used such as in ordering and orientating sequence contigs and in gene cloning.
authors.affiliation: CSIRO Agriculture Flagship, St Lucia, Queensland, Australia Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Ma
authors.affiliation: CSIRO Agriculture Flagship, St Lucia, Queensland, Australia.
authors.forename: Jiri
authors.initials: J
authors.lastname: Stiller
authors.affiliation: Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, China.
authors.forename: Yuming
authors.initials: Y
authors.lastname: Wei
authors.affiliation: Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, China.
authors.forename: You-Liang
authors.initials: YL
authors.lastname: Zheng
authors.affiliation: Department of Crop & Soil Sciences, and Department of Plant Biology, University of Georgia kdevos@uga.edu chunji.liu@csiro.au.
authors.forename: Katrien M
authors.initials: KM
authors.lastname: Devos
authors.affiliation: Centre of the Region Haná for Biotechnological and Agricultural Research, Institute of Experimental Botany, Šlechtitelů, Olomouc, Czech Republic.
authors.forename: Jaroslav
authors.initials: J
authors.lastname: Doležel
authors.affiliation: CSIRO Agriculture Flagship, St Lucia, Queensland, Australia School of Plant Biology, The University of Western Australia, Perth, Western Australia, Australia kdevos@uga.edu chunji.liu@csiro.au.
authors.forename: Chunji
authors.initials: C
authors.lastname: Liu
mesh_terms: D002503:Centromere / Q000235:genetics; D056905:Chromosome Breakpoints; D002872:Chromosome Deletion; D007446:Chromosome Inversion; D032461:Chromosomes, Plant / Q000235:genetics*; D005838:Genotype; D011995:Recombination, Genetic; D014908:Triticum / Q000235:genetics*
keywords: Chinese Spring; chromosomal rearrangement; comparative genomics; pericentric inversion; pericentromeric regions; translocation