title: Construction and Characterization of Recombinant HSV-1 Expressing Early Growth Response-1.
abstract: Early Growth response-1 (Egr-1) is a transcription factor that possesses a variety of biological functions. It has been shown to regulate HSV-1 gene expression and replication in different cellular environments through the recruitment of distinct cofactor complexes. Previous studies demonstrated that Egr-1 can be induced by HSV-1 infection in corneal cells but the level was lower compared to other cell types. The primary goal of this report is to generate a recombinant HSV-1 constitutively expressing Egr-1 and to investigate the regulation of viral replication in different cell types or in animals with Egr-1 overexpression. The approach utilized was to introduce Egr-1 into the BAC system containing complete HSV-1 (F) genome. To assist in the insertion of Egr-1, a gene cassette was constructed that contains the Egr-1 gene flanked byloxP sites. In this clone Egr-1 is expressed under control of CMV immediate-early promoter followed by another gene cassette expressing the enhanced green fluorescent protein (EGFP) under the control of the elongation factor 1α (EF-1 α) promoter. The constructed recombinant viruses were completed containing the Egr-1 gene within the viral genome and the expression was characterized by qRT-PCR and Western blot analyses. Our results showed that Egr-1 transcript and protein can be generated and accumulated upon infection of recombinant virus in Vero and rabbit corneal cells SIRC. This unique virus therefore is useful for studying the effects of Egr-1 during HSV-1 replication and gene regulation in epithelial cells and neurons.
authors.affiliation: Department of Pharmaceutical Sciences, School of Pharmacy, University of Maryland Eastern Shore, 1 College Backbone Road, Princess Anne, MD 21853, USA.
authors.forename: Gautam
authors.initials: G
authors.lastname: Bedadala
authors.affiliation: Department of Pharmaceutical Sciences, School of Pharmacy, University of Maryland Eastern Shore, 1 College Backbone Road, Princess Anne, MD 21853, USA.
authors.forename: Feng
authors.initials: F
authors.lastname: Chen
authors.affiliation: Department of Pharmaceutical Sciences, School of Pharmacy, University of Maryland Eastern Shore, 1 College Backbone Road, Princess Anne, MD 21853, USA.
authors.forename: Robert
authors.initials: R
authors.lastname: Figliozzi
authors.affiliation: Department of Pharmaceutical Sciences, School of Pharmacy, University of Maryland Eastern Shore, 1 College Backbone Road, Princess Anne, MD 21853, USA.
authors.forename: Matthew
authors.initials: M
authors.lastname: Balish
authors.affiliation: Department of Pharmaceutical Sciences, School of Pharmacy, University of Maryland Eastern Shore, 1 College Backbone Road, Princess Anne, MD 21853, USA.
authors.forename: Victor
authors.initials: V
authors.lastname: Hsia