title: The role of EBV in the pathogenesis of Burkitt's Lymphoma: an Italian hospital based survey.
abstract: The exact worldwide incidence of Burkitt's lymphoma is not known. There are three distinct clinical variants of Burkitt's lymphoma, each manifesting differences in epidemiology, clinical presentation, morphology, biology and genetic features: the endemic (African), the sporadic (non-endemic), and the immunodeficiency-associated form. In particular, we reported data regarding Burkitt's lymphoma incidence in the world and across different European countries. Finally, we described clinic-pathological data of 48 Burkitt's lymphomas occurred in Italy from 2003 to 2013, in 4 different hospitals, two of which located in east side, and the other ones located in the west-coast. Forty Burkitt's lymphomas occurs in children (age range 3-12), and 8 were adulthood Burkitt's lymphomas (age range 18-87). In the pediatric group the Male:Female ratio (M:F) was of 4:1, whereas the group of the adult patients has a M:F of 1:1.67. Immunohistochemical detection of Latent Membrane Protein 1 (LMP1) expression and Epstein-Barr virus Encoded RNA (EBER) In Situ Hybridization (ISH) procedures have been performed. Lymphocyte B monoclonal spread has been demonstrated using a Polymerase Chain Reaction (PCR) based method to amplify Fragment Restriction FR1, FR2 and FR3 immunoglobulin heavy chains DNA fragments. Only 38 cases out of 48 were analyzed for LMP-1 showing various percentage of stained cells in 47.4% of the patients. Considering ISH for EBER detection results: 1 out 2 (50%) adult analyzed cases was positive, with 50% of stained tumor cells (this patient was a 22 years old female, coming from Napoli);15 out 24 (62.5%) children analyzed Burkitt's lymphomas resulted as positive for EBER;the overall positivity has been observed in 16/26 Burkitt's lymphomas (61.53%).Finally, EBV has been detected in children and adult patients, one of them with deregulation of the oncogene c-MYC by chromosomal translocation.
authors.affiliation: Department of Clinical and Experimental Medicine, Institute of Pathological Anatomy, University of Foggia, Foggia, Italy.
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Pannone
authors.affiliation: Section of Pathological Anatomy Ospedale di Ascoli, Ascoli Piceno, Italy.
authors.forename: Rosanna
authors.initials: R
authors.lastname: Zamparese
authors.affiliation: Department of Clinical and Experimental Medicine, Institute of Pathological Anatomy, University of Foggia, Foggia, Italy.
authors.forename: Mirella
authors.initials: M
authors.lastname: Pace
authors.affiliation: Department of Clinical and Experimental Medicine, Institute of Pathological Anatomy, University of Foggia, Foggia, Italy.
authors.forename: Maria Carmela
authors.initials: MC
authors.lastname: Pedicillo
authors.affiliation: Department of Clinical and Experimental Medicine, Institute of Pathological Anatomy, University of Foggia, Foggia, Italy.
authors.forename: Simona
authors.initials: S
authors.lastname: Cagiano
authors.affiliation: Section of Pathological Anatomy, Ospedale dei Colli - Monaldi, Napoli, Italy.
authors.forename: Pasquale
authors.initials: P
authors.lastname: Somma
authors.affiliation: Section of Pathological Anatomy, Paediatric Oncological Hospital Pausillipon, Naples, Italy.
authors.forename: Maria Elena
authors.initials: ME
authors.lastname: Errico
authors.affiliation: Section of Pathological Anatomy, Paediatric Oncological Hospital Pausillipon, Naples, Italy.
authors.forename: Vittoria
authors.initials: V
authors.lastname: Donofrio
authors.affiliation: Pathology Unit - Istituto Nazionale dei Tumori, Naples, Italy.
authors.forename: Renato
authors.initials: R
authors.lastname: Franco
authors.affiliation: Pathology Unit - Istituto Nazionale dei Tumori, Naples, Italy.
authors.forename: Annarosaria
authors.initials: A
authors.lastname: De Chiara
authors.affiliation: Pathology Unit - Istituto Nazionale dei Tumori, Naples, Italy.
authors.forename: Gabriella
authors.initials: G
authors.lastname: Aquino
authors.affiliation: Department of Odontostomatological and Maxillofacial Sciences, University of Napoli 'Federico II', Naples, Italy.
authors.forename: Paolo
authors.initials: P
authors.lastname: Bucci
authors.affiliation: Department of Odontostomatological and Maxillofacial Sciences, University of Napoli 'Federico II', Naples, Italy.
authors.forename: Eduardo
authors.initials: E
authors.lastname: Bucci
authors.affiliation: Department of Laboratory, Institute of Histopathology and Diagnostic Cytopathology, Fondazione di Ricerca e Cura 'Giovanni Paolo II'-UCSC, Campobasso, Italy ; Piazza Attilio Omodei Zorini, 48, int.6 00166, Rome, RM Italy.
authors.forename: Angela
authors.initials: A
authors.lastname: Santoro
authors.affiliation: Department of Clinical and Experimental Medicine, Institute of Pathological Anatomy, University of Foggia, Foggia, Italy ; IRCCS CROB - Basilicata Cancer Institute, Rionero in Vulture, Potenza, Italy.
authors.forename: Pantaleo
authors.initials: P
authors.lastname: Bufo
keywords: Burkitt’s lymphoma; Immunohistochemistry; In situ hybridization