title: [Study of association between Parvimonas micra and pulp dominant pathogens in the infected root canals with chronic periradicular periodontitis].
abstract: OBJECTIVE
  To study the prevalence of Parvimonas micra (Pm) and the associations between Pm and pulp dominant pathogens in order to reflect the colonization of Pm in the infected root canals with chronic periradicular periodontitis.
  
  
  METHODS
  A total of 120 teeth diagnosed as chronic periradicular periodontitis from 104 patients were included into the study. The teeth were allocated into untreated (primary infectious) and root-canal- treated (secondary infectious) groups with 60 in either group. Samples were collected from the root canals using sterile files and paper points, and subsequent extraction of bacterial DNA was undertaken. The Pm 16S rDNA level was evaluated using 16S rDNA PCR. The prevalence of Pm in chronic periradicular periodontitis was determined accordingly. Then, the associations of Pm and Enterococcus faecalis (Ef), Porphyromonas endodontalis (Pe) as well as Porphyromonas gingivalis (Pg) were analysed.
  
  
  RESULTS
  Pm was detected in 40% (24/60) of the samples from the primary infectious group, 5% (3/60) from the secondary infectious group. The prevalences of Pm from the two groups were different significantly (χ² = 21.06, P < 0.05). Significant correlations (untreated group OR = 5.98, root-canal-treated group OR = 33.50) between Pm and Pe were identified in both groups, while the correlations between Pm and Pg as well as Ef were not of significance, respectively.
  
  
  CONCLUSIONS
  A significantly higher relevance ratio of Pm was estimated in the primary infectious group than the secondary infectious one. Pm and Pe were correlated significantly in the infected root canals, suggesting a symbiotic relation between these two bacteria.
authors.affiliation: Department of Cariology and Endodontology, Capital Medical University School of Stomatology, Beijing 100050, China.
authors.forename: Hai
authors.initials: H
authors.lastname: Ji
authors.forename: Hong
authors.initials: H
authors.lastname: Li
authors.forename: Yanyan
authors.initials: Y
authors.lastname: He
authors.affiliation: Email: endohou@qq.com.
authors.forename: Benxiang
authors.initials: B
authors.lastname: Hou
mesh_terms: D055113:Chronic Periodontitis; D004269:DNA, Bacterial; D003786:Dental Pulp Cavity / Q000382:microbiology*; D013293:Enterococcus faecalis / Q000302:isolation & purification; D006801:Humans; D010485:Periapical Periodontitis / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D045202:Porphyromonas endodontalis / Q000302:isolation & purification; D016966:Porphyromonas gingivalis / Q000302:isolation & purification; D012390:Root Canal Therapy
chemical_list: D004269:DNA, Bacterial