title: Identification of death receptors DR4 and DR5 in HTB-12 astrocytoma cell lines and determination of TRAIL sensitivity.
abstract: Astrocytomas are tumors which arise from astrocytes, cells that form the blood-brain barrier. There are very few drugs that successfully treat brain tumors. In this study, the cytotoxic effects on the HTB-12 astrocytoma cell line by tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) were studied. The presence of the TRAIL receptors, Death receptor 4 (DR4) and Death receptor 5 (DR5), were detected in HTB-12 cells by Enzyme-Linked Immunosorbent Assay (ELISA). Cytotoxicity assay by Trypan Blue Exclusion Method showed effective cell killing by TRAIL treatment. Thus, the presence of death receptors and TRAIL efficacy raises the therapeutic potential for this type of brain tumor.
authors.affiliation: Department of Biological and Pharmaceutical Sciences, Elizabeth City State University campus of The University of North Carolina, Elizabeth City, NC / USA.
authors.forename: Elenia
authors.initials: E
authors.lastname: Riddick
authors.affiliation: Department of Biological and Pharmaceutical Sciences, Elizabeth City State University campus of The University of North Carolina, Elizabeth City, NC / USA.
authors.forename: Shavonda
authors.initials: S
authors.lastname: Evans
authors.affiliation: Department of Biological and Pharmaceutical Sciences, Elizabeth City State University campus of The University of North Carolina, Elizabeth City, NC / USA.
authors.forename: Jeffrey
authors.initials: J
authors.lastname: Rousch
authors.affiliation: Department of Biological and Pharmaceutical Sciences, Elizabeth City State University campus of The University of North Carolina, Elizabeth City, NC / USA.
authors.forename: Ephraim
authors.initials: E
authors.lastname: Gwebu
authors.affiliation: Department of Biological and Pharmaceutical Sciences, Elizabeth City State University campus of The University of North Carolina, Elizabeth City, NC / USA.
authors.forename: Hirendra Nath
authors.initials: HN
authors.lastname: Banerjee
keywords: Astrocytes; Astrocytoma; Death receptors; TRAIL