title: Formulation, characterization, and in vitro/vivo studies of aclacinomycin A-loaded solid lipid nanoparticles.
abstract: OBJECTIVE
  The aim of this study was to prepare aclacinomycin A (ACM)-loaded solid lipid nanoparticles (SLNs) and to evaluate their in vitro and in vivo characteristics.
  
  
  METHODS
  SLNs were prepared using an emulsion evaporation-solidification method, and characterized in accordance with the morphological examination, particle size distribution, entrapment efficiency, drug-loading, and in vitro release. Pharmacokinetic and biodistribution studies were employed to evaluate the in vivo of SLNs.
  
  
  RESULTS
  The SLNs were spherical in shape, uniform in size, and appropriate for administration via intravenous injection. The drug content, encapsulation efficiency, and drug loading of prepared SLNs were 96.4% ± 4.6%, 86.7% ± 2.3%, and 4.8% ± 0.7% (n = 3), respectively, and the mean diameter was 68.2 ± 5.6 nm from three batches. The SLNs were produced with stable physical properties and demonstrated significantly sustained release. The pharmacokinetic behavior of ACM was greatly improved by lyophilized injection of SLN with sustained drug release and high bioavailability. In addition, the results obtained from tissue distribution showed that ACM-SLNs were hepatic targeting in vivo.
  
  
  CONCLUSIONS
  The present work demonstrated the feasibility of liver-targeted delivery of ACM utilizing SLNs.
authors.affiliation: a Department of General Surgery , Dalian Municipal Center Hospital , Liaoning , China .
authors.forename: Youpeng
authors.initials: Y
authors.lastname: Jia
authors.affiliation: b Department of Central Laboratory , Dalian Municipal Center Hospital , Liaoning , China , and.
authors.forename: Jun
authors.initials: J
authors.lastname: Ji
authors.affiliation: c Department of Pharmaceutics , Shanghai Institute of Pharmaceutical Industry , Shanghai , China.
authors.forename: Feng
authors.initials: F
authors.lastname: Wang
authors.affiliation: a Department of General Surgery , Dalian Municipal Center Hospital , Liaoning , China .
authors.forename: Liangang
authors.initials: L
authors.lastname: Shi
authors.affiliation: a Department of General Surgery , Dalian Municipal Center Hospital , Liaoning , China .
authors.forename: Jingbo
authors.initials: J
authors.lastname: Yu
authors.affiliation: a Department of General Surgery , Dalian Municipal Center Hospital , Liaoning , China .
authors.forename: Dong
authors.initials: D
authors.lastname: Wang
mesh_terms: D015250:Aclarubicin / Q000737:chemistry* / Q000493:pharmacokinetics*; D002626:Chemistry, Pharmaceutical; D003692:Delayed-Action Preparations; D004337:Drug Carriers / Q000493:pharmacokinetics*; D004339:Drug Compounding; D004655:Emulsions / Q000737:chemistry; D008055:Lipids / Q000737:chemistry / Q000493:pharmacokinetics*; D053758:Nanoparticles / Q000737:chemistry*
chemical_list: D003692:Delayed-Action Preparations; D004337:Drug Carriers; D004655:Emulsions; D008055:Lipids; D015250:Aclarubicin
keywords: Aclacinomycin A; biodistribution; in vitro release; pharmacokinetics; solid lipid nanoparticles