title: MOFzyme: Intrinsic protease-like activity of Cu-MOF.
abstract: The construction of efficient enzyme mimetics for the hydrolysis of peptide bonds in proteins is challenging due to the high stability of peptide bonds and the importance of proteases in biology and industry. Metal-organic frameworks (MOFs) consisting of infinite crystalline lattices with metal clusters and organic linkers may provide opportunities for protease mimic which has remained unknown. Herein, we report that Cu₂(C₉H₃O₆)₄/₃ MOF (which is well known as HKUST-1 and denoted as Cu-MOF here), possesses an intrinsic enzyme mimicking activity similar to that found in natural trypsin to bovine serum albumin (BSA) and casein. The Michaelis constant (Km) of Cu-MOF is about 26,000-fold smaller than that of free trypsin indicating a much higher affinity of BSA for Cu-MOF surface. Cu-MOF also exhibited significantly higher catalytic efficiency than homogeneous artificial metalloprotease Cu(II) complexes and could be reused for ten times without losing in its activity. Moreover, Cu-MOF was successfully used to simulate trypsinization in cell culture since it dissociated cells in culture even without EDTA.
authors.affiliation: Yunnan Engineering Research Center of Photocatalytic Treatment of Industrial Wastewater, The Universities' Center for Photocatalytic Treatment of Pollutants in Yunnan Province, Key Laboratory of Medicinal Chemistry for Natural Resource, Ministry of Education, School of Chemical Sciences &Technology, Yunnan University, Kunming 650091, P.R. China.
authors.forename: Bin
authors.initials: B
authors.lastname: Li
authors.affiliation: Yunnan Engineering Research Center of Photocatalytic Treatment of Industrial Wastewater, The Universities' Center for Photocatalytic Treatment of Pollutants in Yunnan Province, Key Laboratory of Medicinal Chemistry for Natural Resource, Ministry of Education, School of Chemical Sciences &Technology, Yunnan University, Kunming 650091, P.R. China.
authors.forename: Daomei
authors.initials: D
authors.lastname: Chen
authors.affiliation: Yunnan Engineering Research Center of Photocatalytic Treatment of Industrial Wastewater, The Universities' Center for Photocatalytic Treatment of Pollutants in Yunnan Province, Key Laboratory of Medicinal Chemistry for Natural Resource, Ministry of Education, School of Chemical Sciences &Technology, Yunnan University, Kunming 650091, P.R. China.
authors.forename: Jiaqiang
authors.initials: J
authors.lastname: Wang
authors.affiliation: Yunnan Engineering Research Center of Photocatalytic Treatment of Industrial Wastewater, The Universities' Center for Photocatalytic Treatment of Pollutants in Yunnan Province, Key Laboratory of Medicinal Chemistry for Natural Resource, Ministry of Education, School of Chemical Sciences &Technology, Yunnan University, Kunming 650091, P.R. China.
authors.forename: Zhiying
authors.initials: Z
authors.lastname: Yan
authors.affiliation: Yunnan Engineering Research Center of Photocatalytic Treatment of Industrial Wastewater, The Universities' Center for Photocatalytic Treatment of Pollutants in Yunnan Province, Key Laboratory of Medicinal Chemistry for Natural Resource, Ministry of Education, School of Chemical Sciences &Technology, Yunnan University, Kunming 650091, P.R. China.
authors.forename: Liang
authors.initials: L
authors.lastname: Jiang
authors.affiliation: Yunnan Engineering Research Center of Photocatalytic Treatment of Industrial Wastewater, The Universities' Center for Photocatalytic Treatment of Pollutants in Yunnan Province, Key Laboratory of Medicinal Chemistry for Natural Resource, Ministry of Education, School of Chemical Sciences &Technology, Yunnan University, Kunming 650091, P.R. China.
authors.lastname: Deliang Duan
authors.affiliation: Yunnan Engineering Research Center of Photocatalytic Treatment of Industrial Wastewater, The Universities' Center for Photocatalytic Treatment of Pollutants in Yunnan Province, Key Laboratory of Medicinal Chemistry for Natural Resource, Ministry of Education, School of Chemical Sciences &Technology, Yunnan University, Kunming 650091, P.R. China.
authors.forename: Jiao
authors.initials: J
authors.lastname: He
authors.affiliation: Yunnan Engineering Research Center of Photocatalytic Treatment of Industrial Wastewater, The Universities' Center for Photocatalytic Treatment of Pollutants in Yunnan Province, Key Laboratory of Medicinal Chemistry for Natural Resource, Ministry of Education, School of Chemical Sciences &Technology, Yunnan University, Kunming 650091, P.R. China.
authors.forename: Zhongrui
authors.initials: Z
authors.lastname: Luo
authors.affiliation: Yunnan Engineering Research Center of Photocatalytic Treatment of Industrial Wastewater, The Universities' Center for Photocatalytic Treatment of Pollutants in Yunnan Province, Key Laboratory of Medicinal Chemistry for Natural Resource, Ministry of Education, School of Chemical Sciences &Technology, Yunnan University, Kunming 650091, P.R. China.
authors.forename: Jinping
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Yunnan Engineering Research Center of Photocatalytic Treatment of Industrial Wastewater, The Universities' Center for Photocatalytic Treatment of Pollutants in Yunnan Province, Key Laboratory of Medicinal Chemistry for Natural Resource, Ministry of Education, School of Chemical Sciences &Technology, Yunnan University, Kunming 650091, P.R. China.
authors.forename: Fagui
authors.initials: F
authors.lastname: Yuan
mesh_terms: D002364:Caseins / Q000737:chemistry / Q000378:metabolism; D002384:Catalysis; D002460:Cell Line; D010450:Endopeptidases / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D007700:Kinetics; D000073396:Metal-Organic Frameworks; D009942:Organometallic Compounds / Q000737:chemistry / Q000378:metabolism*; D059748:Proteolysis; D012710:Serum Albumin, Bovine / Q000737:chemistry / Q000378:metabolism
chemical_list: D002364:Caseins; D000073396:Metal-Organic Frameworks; D009942:Organometallic Compounds; C539834:bis(1,3,5-benzenetricarboxylate)tricopper(II); D012710:Serum Albumin, Bovine; D010450:Endopeptidases