title: A new quadrannulate species of Orobdella (Hirudinida, Arhynchobdellida, Orobdellidae) from central Honshu, Japan.
abstract: A new quadrannulate species of Orobdella, Orobdellamasaakikuroiwai sp. n., from the mountainous region of central Honshu, Japan is described. This is only the second small species known within this genus, with a body length of less than 4 cm for mature individuals. Phylogenetic analyses using nuclear 18S rDNA and histone H3 as well as mitochondrial COI, tRNA(Cys), tRNA(Met), 12S, tRNA(Val), 16S, and ND1 markers showed that Orobdellamasaakikuroiwai sp. n. is the sister species of the quadrannulate Orobdellawhitmani Oka, 1895. Phylogenetic relationships within Orobdellamasaakikuroiwai sp. n. conducted using mitochondrial markers reveled a distinction between eastern and western phylogroups.
authors.affiliation: Department of Zoology, Graduate School of Science, Kyoto University, Kyoto 606-8502, Japan.
authors.forename: Takafumi
authors.initials: T
authors.lastname: Nakano
keywords: Hirudinea; Hirudinida; Japan; Orobdella; gastroporous; molecular phylogeny; new species