title: Anticancer activity of the phytomedicine DAS-77.
abstract: This study was designed to investigate the anticancer activity of extracts of the phytomedicine DAS-77. The sulforhodamine B (SRB) in vitro cytotoxicity assay, Sarcoma-180 (S-180) ascites and solid tumor, and L1210 lymphoid leukemia in vivo models were employed. DAS-A001 (ethanol extract, IC50 12 and 13 µg/mL with HCT-116 and PC3, respectively); DAS-A002 (hydroethanol extract, IC50 <5 and 13 µg/mL with HCT-116 and PC3, respectively); DAS-A003 (aqueous extract, IC50 <5 µg/mL with THP-1); and DAS-A004 (dichloromethane:methanol extract; IC50 <5 and 17 µg/mL with HCT-116 and PC3, respectively) demonstrated significant activity in vitro. DAS-A002 and DAS-A003 (80-120 mg/kg) elicited significant (P < .05-.001) dose-dependent inhibition of tumor growth in the S-180 ascites model. Peak effects were produced at the highest dose of 120 mg/kg with inhibition values of 87.50% and 89.23% for DAS-A002 and DAS-A003, respectively, compared with a value of 97.27% for 5-FU (20 mg/kg). As regards the S-180 solid tumor model, inhibition of tumor growth was found to be 52.56% and 37.95%, respectively, for DAS-A002 and DAS-A003. The effect of DAS-A002 was comparable and not significantly different (P > .05) from that of 5-FU (20 mg/kg; 50.18% inhibition). DAS-A003 but not DAS-A002 showed significant activity in the leukemia model with 177.78% increase in mean survival time relative to 211.11% for 5-FU. Findings in this study suggest that the hydroethanol and aqueous extracts of DAS-77 possess significant anticancer activity.
authors.affiliation: Cancer Pharmacology Division, Indian Institute of Integrative Medicine, Council of Scientific & Industrial Research, Jammu-Tawi, Jammu & Kashmir, India Natural Products Chemistry Division, Indian Institute of Integrative Medicine, Council of Scientific & Industrial Research, Jammu-Tawi, Jammu & Kashmir, India Department of Pharmacology, Therapeutics & Toxicology, Faculty of Basic Medical Sciences, College of Medicine, University of Lagos, Lagos, Nigeria ajakindele@cmul.edu.ng.
authors.forename: Abidemi J
authors.initials: AJ
authors.lastname: Akindele
authors.affiliation: Cancer Pharmacology Division, Indian Institute of Integrative Medicine, Council of Scientific & Industrial Research, Jammu-Tawi, Jammu & Kashmir, India.
authors.forename: Girish
authors.initials: G
authors.lastname: Mahajan
authors.affiliation: Cancer Pharmacology Division, Indian Institute of Integrative Medicine, Council of Scientific & Industrial Research, Jammu-Tawi, Jammu & Kashmir, India.
authors.forename: Zahoor A
authors.initials: ZA
authors.lastname: Wani
authors.affiliation: Cancer Pharmacology Division, Indian Institute of Integrative Medicine, Council of Scientific & Industrial Research, Jammu-Tawi, Jammu & Kashmir, India.
authors.forename: Sadhana
authors.initials: S
authors.lastname: Sharma
authors.affiliation: Cancer Pharmacology Division, Indian Institute of Integrative Medicine, Council of Scientific & Industrial Research, Jammu-Tawi, Jammu & Kashmir, India.
authors.forename: Naresh K
authors.initials: NK
authors.lastname: Satti
authors.affiliation: Cancer Pharmacology Division, Indian Institute of Integrative Medicine, Council of Scientific & Industrial Research, Jammu-Tawi, Jammu & Kashmir, India.
authors.forename: Olufunmilayo O
authors.initials: OO
authors.lastname: Adeyemi
authors.affiliation: Cancer Pharmacology Division, Indian Institute of Integrative Medicine, Council of Scientific & Industrial Research, Jammu-Tawi, Jammu & Kashmir, India.
authors.forename: Dilip M
authors.initials: DM
authors.lastname: Mondhe
authors.affiliation: Cancer Pharmacology Division, Indian Institute of Integrative Medicine, Council of Scientific & Industrial Research, Jammu-Tawi, Jammu & Kashmir, India.
authors.forename: Ajit K
authors.initials: AK
authors.lastname: Saxena
mesh_terms: D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000494:pharmacology*; D045744:Cell Line, Tumor / Q000187:drug effects; D004354:Drug Screening Assays, Antitumor; D006801:Humans; D020128:Inhibitory Concentration 50; D051379:Mice; D008807:Mice, Inbred BALB C; D008811:Mice, Inbred DBA; D008517:Phytotherapy; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D012510:Sarcoma 180 / Q000188:drug therapy*
chemical_list: D000972:Antineoplastic Agents, Phytogenic; C581152:DAS-77; D010936:Plant Extracts
keywords: DAS-77; anticancer; cytotoxicity; human cancer cell lines; lymphoid leukemia; solid tumor