title: The interactive effects of mercury and selenium on metabolic profiles, gene expression and antioxidant enzymes in halophyte Suaeda salsa.
abstract: Suaeda salsa is the pioneer halophyte in the Yellow River Delta and was consumed as a popular vegetable. Mercury has become a highly risky contaminant in the sediment of intertidal zones of the Yellow River Delta. In this work, we investigated the interactive effects of mercury and selenium in S. salsa on the basis of metabolic profiling, antioxidant enzyme activities and gene expression quantification. Our results showed that mercury exposure (20 μg L(-1)) inhibited plant growth of S. salsa and induced significant metabolic responses and altered expression levels of INPS, CMO, and MDH in S. salsa samples, together with the increased activities of antioxidant enzymes including SOD and POD. Overall, these results indicated osmotic and oxidative stresses, disturbed protein degradation and energy metabolism change in S. salsa after mercury exposures. Additionally, the addition of selenium could induce both antagonistic and synergistic effects including alleviating protein degradation and aggravating osmotic stress caused by mercury.
authors.affiliation: School of Life Sciences, Ludong University, Yantai 264025, People's Republic of China.
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Liu
authors.affiliation: School of Life Sciences, Ludong University, Yantai 264025, People's Republic of China.
authors.forename: Yongkai
authors.initials: Y
authors.lastname: Lai
authors.affiliation: School of Life Sciences, Ludong University, Yantai 264025, People's Republic of China.
authors.forename: Hushan
authors.initials: H
authors.lastname: Sun
authors.affiliation: School of Life Sciences, Ludong University, Yantai 264025, People's Republic of China.
authors.forename: Yiyan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: School of Life Sciences, Ludong University, Yantai 264025, People's Republic of China.
authors.forename: Ning
authors.initials: N
authors.lastname: Zou
mesh_terms: D019663:Chenopodiaceae / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D015870:Gene Expression / Q000187:drug effects; D050538:Malate Dehydrogenase (NADP+) / Q000235:genetics; D008628:Mercury / Q000633:toxicity*; D055442:Metabolome; D055432:Metabolomics; D007296:Myo-Inositol-1-Phosphate Synthase / Q000235:genetics; D018384:Oxidative Stress; D010105:Oxygenases / Q000235:genetics; D009195:Peroxidase / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D055051:Salt-Tolerant Plants / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D012643:Selenium / Q000494:pharmacology*; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D010940:Plant Proteins; C105448:choline monooxygenase; D050538:Malate Dehydrogenase (NADP+); D009195:Peroxidase; D010105:Oxygenases; D013482:Superoxide Dismutase; D007296:Myo-Inositol-1-Phosphate Synthase; D008628:Mercury; D012643:Selenium
keywords: Suaeda salsa; gene expression; mercury; metabolomics; selenium