title: A target-triggered exponential amplification-based DNAzyme biosensor for ultrasensitive detection of folate receptors.
abstract: We develop a new method for ultrasensitive detection of folate receptors (FRs) using a target-triggered isothermally exponential amplification reaction (EXPAR)-based DNAzyme biosensor. This method exhibits excellent specificity and high sensitivity with a detection limit as low as 0.23 fM and a large dynamic range of 6 orders of magnitude from 1 fM to 1 nM. It might be further applied for the detection of various small molecule-binding proteins by simply changing the linked small molecule moiety of the hairpin probes.
authors.affiliation: Single-Molecule Detection and Imaging Laboratory, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China. zhangcy@siat.ac.cn.
authors.forename: Li-juan
authors.initials: LJ
authors.lastname: Wang
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.forename: Chun-yang
authors.initials: CY
authors.lastname: Zhang
mesh_terms: D015374:Biosensing Techniques; D045744:Cell Line, Tumor; D021881:DNA, Catalytic / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry; D058973:Folate Receptors, GPI-Anchored / Q000032:analysis* / Q000737:chemistry; D054856:G-Quadruplexes; D006367:HeLa Cells; D006801:Humans; D011524:Protoporphyrins / Q000737:chemistry; D044967:Serum / Q000737:chemistry; D013050:Spectrometry, Fluorescence
chemical_list: D021881:DNA, Catalytic; D005456:Fluorescent Dyes; D058973:Folate Receptors, GPI-Anchored; D011524:Protoporphyrins; C017803:zinc protoporphyrin