title: Generation of Mammalian offspring by haploid embryonic stem cells microinjection.
abstract: In this unit we introduce the derivation and genetic modification of mouse haploid embryonic stem (ES) cells. We detail how to produce haploid embryos and the subsequent ES derivation and cell culture. We further introduce readers to the intracytoplasmic injection processes of two types of haploid ES cells [androgenetic haploid ES (ahES) and parthenogenetic ES (phES)], both of which possess potential to produce fertile progenies by microinjection. This unit will be interesting to researchers who focus on recessive screens and transgenic animal model production with haploid stem cells.
authors.affiliation: State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Shuai
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.forename: Haifeng
authors.initials: H
authors.lastname: Wan
authors.forename: Xiao-Yang
authors.initials: XY
authors.lastname: Zhao
authors.forename: Liu
authors.initials: L
authors.lastname: Wang
authors.forename: Qi
authors.initials: Q
authors.lastname: Zhou
mesh_terms: D000818:Animals; D004622:Embryo, Mammalian / Q000166:cytology / Q000378:metabolism*; D051379:Mice; D008845:Microinjections / Q000379:methods*; D000066450:Mouse Embryonic Stem Cells / Q000166:cytology / Q000378:metabolism*; D010312:Parthenogenesis
keywords: genetic modification; haploid embryonic stem cells; microinjection; recessive screens