title: Effects of Sucrose, Phosphate, and Calcium Carbonate on the Production of Pikromycin from Streptomyces venezuelae.
abstract: Polyketide secondary metabolites share common precursor pools, acyl-CoA. Thus, the effects of engineering strategies for heterologous and native secondary metabolite production are often determined by the measurement of pikromycin in Streptomyces venezuelae. It is hard to compare the effectiveness of engineering targets among published data owing to the different pikromycin production media used from one study to the other. To determine the most important nutritional factor and establish optimal culture conditions, medium optimization of pikromycin from Streptomyces venezuelae ATCC 15439 was studied with a statistical method, Plackett-Burman design. Nine variables (glucose, sucrose, peptone, (NH4)2SO4, K2HPO4, KH2PO4, NaCl, MgSO4·7H2O, and CaCO3) were analyzed for their effects on a response, pikromycin. Glucose, K2HPO4, and CaCO3 were determined to be the most significant factors. The path of the steepest ascent and response surface methodology about the three selected components were performed to study interactions among the three factors, and the fine-tune concentrations for maximized product yields. The significant variables and optimal concentrations were 139 g/1 sucrose, 5.29 g/l K2HPO4, and 0.081 g/l CaCO3, with the maximal pikromycin yield of 35.5 mg/l. Increases of the antibiotics production by 1.45-fold, 1.3-fold, and 1.98-fold, compared with unoptimized medium and two other pikromycin production media SCM and SGGP, respectively, were achieved.
authors.affiliation: School of Chemical and Biological Engineering, Institute of Molecular Biology and Genetics, and Bioengineering Institute, Seoul National University, Seoul 151-744, Republic of Korea.
authors.forename: Jeong Sang
authors.initials: JS
authors.lastname: Yi
authors.affiliation: School of Chemical and Biological Engineering, Institute of Molecular Biology and Genetics, and Bioengineering Institute, Seoul National University, Seoul 151-744, Republic of Korea.
authors.forename: Min-Suk
authors.initials: MS
authors.lastname: Kim
authors.affiliation: Division of Life Sciences, Korea Polar Research Institute, Incheon 406-840, Republic of Korea.|Interdisciplinary Program for Bioengineering, Seoul National University, Seoul 152-742, Republic of Korea.
authors.forename: Sung-Jin
authors.initials: SJ
authors.lastname: Kim
authors.affiliation: School of Chemical and Biological Engineering, Institute of Molecular Biology and Genetics, and Bioengineering Institute, Seoul National University, Seoul 151-744, Republic of Korea.|Interdisciplinary Program for Biochemical Engineering and Biotechnology, Seoul National University, Seoul 151-742, Republic of Korea.
authors.forename: Byung-Gee
authors.initials: BG
authors.lastname: Kim
mesh_terms: D002119:Calcium Carbonate / Q000378:metabolism*; D005285:Fermentation; D018942:Macrolides / Q000378:metabolism*; D010710:Phosphates / Q000378:metabolism*; D064210:Secondary Metabolism; D013302:Streptomyces / Q000378:metabolism*; D013395:Sucrose / Q000378:metabolism*
chemical_list: D018942:Macrolides; D010710:Phosphates; D013395:Sucrose; C003145:picromycin; D002119:Calcium Carbonate