title: The superoxide dismutase from red claw crayfish, Cherax quadricarinatus: molecular cloning and characterization analysis.
abstract: In the present study, an extracellular copper-zinc superoxide dismutase (ecCuZnSOD) gene and a mitochondrial manganese superoxide dismutase (mtMnSOD) gene were cloned from hemocytes of red claw crayfish, Cherax quadricarinatus. The open reading frame (ORF) of ecCuZnSOD is 498 bp and encodes a 166 amino acids (aa) protein, whereas the ORF of mtMnSOD is 654 bp and encodes a 218 aa protein. The amino acid sequences of C. quadricarinatus ecCuZnSOD and mtMnSOD showed high similarities with those of ecCuZnSODs and mtMnSODs of other crustaceans, respectively. Both ecCuZnSOD and mtMnSOD of C. quadricarinatus were highly expressed in hepatopancreas, hemocytes, intestine, and gill; low transcript levels were seen in other tissues (heart, muscle, and nerve). The immune responses of ecCuZnSOD and mtMnSOD were studied following inoculation with Spiroplasma eriocheiris and Aeromonas hydrophila. After S. eriocheiris or A. hydrophila challenge, mRNA transcription of ecCuZnSOD and mtMnSOD in hemocytes and gill was upregulated. mRNA transcription of ecCuZnSOD in the hepatopancreas was also upregulated after S. eriocheiris or A. hydrophila inoculation. mtMnSOD in hepatopancreas was upregulated after A. hydrophila inoculation, whereas this was down-regulated after S. eriocheiris challenge. After S. eriocheiris and A. hydrophila challenge, total SOD activity and CuZnSOD activity both increased compared to control group. The results showed that these SODs from C. quadricarinatus likely play an important role in protecting some tissues from reactive oxygen intermediates produced during challenge from S. eriocheiris and A. hydrophila.
authors.affiliation: 1 Jiangsu Key Laboratory for Biodiversity & Biotechnology and Jiangsu Key Laboratory for Aquatic Crustacean Diseases, College of Life Sciences, Nanjing Normal University, Nanjing 210023, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Gu
authors.forename: Jing
authors.initials: J
authors.lastname: Chen
authors.forename: Libo
authors.initials: L
authors.lastname: Hou
authors.forename: Yanqing
authors.initials: Y
authors.lastname: Huang
authors.forename: Siyao
authors.initials: S
authors.lastname: Xia
authors.forename: Qingguo
authors.initials: Q
authors.lastname: Meng
authors.forename: Wen
authors.initials: W
authors.lastname: Wang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D003400:Astacoidea / Q000201:enzymology*; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D015971:Gene Expression Regulation, Enzymologic; D008297:Male; D008969:Molecular Sequence Data; D010802:Phylogeny; D013482:Superoxide Dismutase / Q000235:genetics / Q000378:metabolism*
chemical_list: D018076:DNA, Complementary; D013482:Superoxide Dismutase
keywords: extracellular copper/zinc superoxide dismutase; mitochondrial manganese superoxide dismutase,Cherax quadricarinatus