title: Halorhodopsin pumps Cl- and bacteriorhodopsin pumps protons by a common mechanism that uses conserved electrostatic interactions.
abstract: Key mutations differentiate the functions of homologous proteins. One example compares the inward ion pump halorhodopsin (HR) and the outward proton pump bacteriorhodopsin (BR). Of the nine essential buried ionizable residues in BR, six are conserved in HR. However, HR changes three BR acids, D85 in a central cluster of ionizable residues, D96, nearer the intracellular, and E204, nearer the extracellular side of the membrane to the small, neutral amino acids T111, V122, and T230, respectively. In BR, acidic amino acids are stationary anions whose proton affinity is modulated by conformational changes, establishing a sequence of directed binding and release of protons. Multiconformation continuum electrostatics calculations of chloride affinity and residue protonation show that, in reaction intermediates where an acid is ionized in BR, a Cl(-) is bound to HR in a position near the deleted acid. In the HR ground state, Cl(-) binds tightly to the central cluster T111 site and weakly to the extracellular T230 site, recovering the charges on ionized BR-D85 and neutral E204 in BR. Imposing key conformational changes from the BR M intermediate into the HR structure results in the loss of Cl(-) from the central T111 site and the tight binding of Cl(-) to the extracellular T230 site, mirroring the changes that protonate BR-D85 and ionize E204 in BR. The use of a mobile chloride in place of D85 and E204 makes HR more susceptible to the environmental pH and salt concentrations than BR. These studies shed light on how ion transfer mechanisms are controlled through the interplay of protein and ion electrostatics.
authors.affiliation: Cyrus Biotechnology, Inc., Seattle, WA 98101; and.
authors.forename: Yifan
authors.identifier: http://orcid.org/0000-0001-8064-3564
authors.initials: Y
authors.lastname: Song
authors.affiliation: Physics Department, City College of New York, New York, NY 10031 gunner@sci.ccny.cuny.edu.
authors.forename: M R
authors.initials: MR
authors.lastname: Gunner
mesh_terms: D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D000596:Amino Acids / Q000737:chemistry; D001436:Bacteriorhodopsins / Q000737:chemistry / Q000378:metabolism*; D001665:Binding Sites; D020134:Catalytic Domain; D002712:Chlorides / Q000378:metabolism*; D017124:Conserved Sequence; D019617:Halobacterium salinarum / Q000378:metabolism; D025602:Halorhodopsins / Q000737:chemistry / Q000378:metabolism*; D017136:Ion Transport; D008958:Models, Molecular; D056004:Molecular Dynamics Simulation; D008969:Molecular Sequence Data; D009010:Monte Carlo Method; D011487:Protein Conformation; D011522:Protons; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity; D055672:Static Electricity
chemical_list: D000596:Amino Acids; D002712:Chlorides; D025602:Halorhodopsins; D011522:Protons; D001436:Bacteriorhodopsins
keywords: MCCE; buried charge; continuum electrostatics; ion binding; pKa