title: The use of MYBL2 as a novel candidate biomarker of cervical cancer.
abstract: Cervical cancer is the third most common cancer affecting women worldwide. It is characterized by chromosomal aberrations and alteration in the expression levels of many cell cycle regulatory proteins, driven primarily by transforming human papillomavirus (HPV) infection. MYBL2 is a member of the MYB proto-oncogene family that encodes DNA binding proteins. These proteins are involved in cell proliferation and control of cellular differentiation. We have previously demonstrated the utility of MYBL2 as a putative biomarker for cervical pre-cancer and cancer. In this chapter we describe the methodological approach for testing MYBL2 protein expression in tissue biopsies from cases of cervical intraepithelial neoplasia (CIN) and cervical cancer, using immunohistochemistry techniques on the automated immunostaining platform, the Ventana BenchMark LT. The protocol outlines the various steps in the procedure from cutting tissue sections, antibody optimization, antigen retrieval, immunostaining, and histological review.
authors.affiliation: Department of Histopathology, University of Dublin, Trinity College, Dublin, Ireland, cara.martin@tcd.ie.
authors.forename: Cara M
authors.initials: CM
authors.lastname: Martin
authors.forename: Katharine
authors.initials: K
authors.lastname: Astbury
authors.forename: Louise
authors.initials: L
authors.lastname: Kehoe
authors.forename: Jacqueline Barry
authors.initials: JB
authors.lastname: O'Crowley
authors.forename: Sharon
authors.initials: S
authors.lastname: O'Toole
authors.forename: John J
authors.initials: JJ
authors.lastname: O'Leary
mesh_terms: D014408:Biomarkers, Tumor / Q000378:metabolism*; D018797:Cell Cycle Proteins / Q000378:metabolism*; D045744:Cell Line, Tumor; D005260:Female; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods*; D016612:Paraffin Embedding; D000090063:Proto-Oncogene Mas; D016707:Tissue Fixation; D015534:Trans-Activators / Q000378:metabolism*; D002583:Uterine Cervical Neoplasms / Q000378:metabolism*
chemical_list: D014408:Biomarkers, Tumor; D018797:Cell Cycle Proteins; C000715470:MAS1 protein, human; C064352:MYBL2 protein, human; D000090063:Proto-Oncogene Mas; D015534:Trans-Activators