title: Detection of rotavirus using padlock probes and rolling circle amplification.
abstract: Rotavirus infections are one of the most common reasons for hospitalizations due to gastrointestinal diseases. Rotavirus is often diagnosed by latex agglutination assay, chromatography immunoassay, or by electron microscopy, which are all quite insensitive. Reverse transcription polymerase chain reaction, on the other hand, is very sensitive to variations at the genomic level. We developed a novel assay based on a set of 58 different padlock probes with a detection limit of 1,000 copies. Twenty-two patient samples were analyzed and the assay showed high concordance with a PCR-based assay. In summary, we present a new assay for sensitive and variation tolerant detection of rotavirus.
authors.affiliation: Science for Life Laboratory, Department of Biochemistry and Biophysics, Stockholm University, Solna, Sweden.
authors.forename: Anja
authors.initials: A
authors.lastname: Mezger
authors.affiliation: Section of Clinical Virology, Department of Medical Sciences, Uppsala University Hospital, Uppsala, Sweden.
authors.forename: Christina
authors.initials: C
authors.lastname: Öhrmalm
authors.affiliation: Science for Life Laboratory, Department of Biochemistry and Biophysics, Stockholm University, Solna, Sweden.
authors.forename: David
authors.initials: D
authors.lastname: Herthnek
authors.affiliation: Section of Clinical Virology, Department of Medical Sciences, Uppsala University Hospital, Uppsala, Sweden.
authors.forename: Jonas
authors.initials: J
authors.lastname: Blomberg
authors.affiliation: Science for Life Laboratory, Department of Biochemistry and Biophysics, Stockholm University, Solna, Sweden.
authors.forename: Mats
authors.initials: M
authors.lastname: Nilsson
mesh_terms: D001483:Base Sequence; D015342:DNA Probes / Q000235:genetics; D006801:Humans; D057230:Limit of Detection; D025202:Molecular Diagnostic Techniques; D008969:Molecular Sequence Data; D021141:Nucleic Acid Amplification Techniques; D012401:Rotavirus / Q000235:genetics*; D012400:Rotavirus Infections / Q000175:diagnosis* / Q000821:virology
chemical_list: D015342:DNA Probes