title: [Prediction and identification of linear B-cell epitopes in major group 3 allergen derived from Dermatophagoides farina].
abstract: OBJECTIVE
  To predict and identify the linear B-cell epitopes in the major group 3 allergen derived from Dermatophagoides farina (Der f 3).
  
  
  METHODS
  The linear B-cell epitopes of Der f 3 allergen were analyzed based on the physicochemical properties of amino acids including antigenicity, surface accessibility, flexibility, hydrophilicity, beta-turn by online bioinformatics softwares. The eight predicted peptides of linear B-cell epitopes were artificially synthesized and incubated with three aller gic serum pools (4 serum samples in each), which were consisted of total 12 serum samples from the allergic individuals, and the strong positive epitopes were selected.
  
  
  RESULTS
  Eight B-cell epitopes from Der f 3 were predicted successfully. Five of eight B-cell epitopes were identified with strong IgE-binding abilities followed by specific IgE assay. The amino acid sequences of them were following: 33KAKAGDCP40, 86HASGGEKIQVAEIYQHENYDSMTID110, 118LKTPMTLDQTNAKPVPLPPQGSDVKVG144, 156QEGSYSLP163 and 199DVANGGVDSCQGDSGGPVVD218.
  
  
  CONCLUSIONS
  Five linear B-cell epitopes of Der f 3 allergen have been identified successfully. This result might provide a basis of the diagnosis and treatment for asthma.
authors.forename: Na
authors.initials: N
authors.lastname: Li
authors.forename: Chao-pin
authors.initials: CP
authors.lastname: Li
authors.forename: Ji-dong
authors.initials: JD
authors.lastname: Diao
authors.forename: Bei-bei
authors.initials: BB
authors.lastname: Zhao
authors.forename: Wei
authors.initials: W
authors.lastname: Lu
authors.forename: Yu-xin
authors.initials: YX
authors.lastname: Jiang
mesh_terms: D000485:Allergens / Q000737:chemistry / Q000276:immunology*; D000595:Amino Acid Sequence; D000818:Animals; D001249:Asthma / Q000097:blood / Q000276:immunology; D018604:Epitope Mapping; D018985:Epitopes, B-Lymphocyte / Q000737:chemistry* / Q000276:immunology*; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D007073:Immunoglobulin E / Q000276:immunology; D008969:Molecular Sequence Data; D039981:Pyroglyphidae / Q000276:immunology*; D013499:Surface Properties
chemical_list: D000485:Allergens; D018985:Epitopes, B-Lymphocyte; D007073:Immunoglobulin E