title: Epigallocatechin-3-Gallate Protects Erythrocyte Ca(2+)-ATPase and Na(+)/K(+)-ATPase Against Oxidative Induced Damage During Aging in Humans.
abstract: OBJECTIVE
  The main purpose of this study was to investigate the protective role of epigallocatechin-3-gallate on tertiary butyl hydroperoxide induced oxidative damage in erythrocyte during aging in humans.
  
  
  METHODS
  Human erythrocyte membrane bound Ca(2+)-ATPase and Na(+)/K(+)-ATPase activities were determined as a function of human age. Protective role of epigallocatechin-3-gallate was evaluated by in vitro experiments by adding epigallocatechin-3-gallate in concentration dependent manner (final concentration range 10(-7)M to 10(-4)M) to the enzyme assay medium. Oxidative stress was induced in vitro by incubating washed erythrocyte ghosts with tertiary butyl hydroperoxide (10(-5) M final concentration).
  
  
  RESULTS
  We have reported concentration dependent effect of epigallocatechin-3-gallate on tertiary butyl hydroperoxide induced damage on activities of Ca(2+)-ATPase and Na(+)/K(+)-ATPase during aging in humans. We have detected a significant (p < 0.001) decreased activity of Ca(2+)-ATPase and Na(+)/K(+) -ATPase as a function of human age. Epigallocatechin-3-gallate protected ATPases against tertiary butyl hydroperoxide induced damage in concentration dependent manner during aging in humans.
  
  
  CONCLUSIONS
  Epigallocatechin-3-gallate is a powerful antioxidant that is capable of protecting erythrocyte Ca(2+)-ATPase and Na(+)/K(+) -ATPase against oxidative stress during aging in humans. We may propose hypothesis that a high intake of catechin rich diet may provide some protection against development of aging and age related diseases.
authors.affiliation: Amity Institute of Biotechnology, Amity University Uttar Pradesh, Noida, 201303, India.
authors.forename: Prabhanshu
authors.initials: P
authors.lastname: Kumar
authors.affiliation: Amity Institute of Biotechnology, Amity University Uttar Pradesh, Noida, 201303, India.
authors.forename: Pawan Kumar
authors.initials: PK
authors.lastname: Maurya
keywords: Aging; Ca2+-ATPase; EGCG; Erythrocytes; Na+/K+ -ATPase; Oxidative stress