title: MicroRNA signatures in chemotherapy resistant esophageal cancer cell lines.
abstract: OBJECTIVE
  To investigate expression of microRNA (miRNA) and potential targets in chemotherapy resistant esophageal cancer cell lines.
  
  
  METHODS
  An in-vitro model of acquired chemotherapy resistance in esophageal adeno- (EAC) and squamous cell carcinoma (ESCC) cells was used, and microRNA expression profiles for cisplatin or 5-fluorouracil (5-FU) resistant variants vs chemotherapy sensitive controls were compared using microarray and quantitative real-time polymerase chain reaction (PCR). The expression of chemotherapy-relevant genes potentially targeted by the dysregulated microRNAs in the chemotherapy resistant variants was also evaluated.
  
  
  RESULTS
  Chemotherapy resistant sublines were found to have specific miRNA signatures, and these miRNA signatures were different for the cisplatin vs 5-FU resistant cells from the same tumor cell line, and also for EAC vs ESCC cells with resistance to the same specific chemotherapy agent. Amongst others, miR-27b-3p, miR-193b-3p, miR-192-5p, miR-378 a-3p, miR-125a-5p and miR-18a-3p were dysregulated, consistent with negative posttranscriptional control of KRAS, TYMS, ABCC3, CBL-B and ERBB2 expression via these miRNAs.
  
  
  CONCLUSIONS
  The current study supports the hypothesis that microRNA expression has an impact on chemotherapy resistance in esophageal cancer.
authors.affiliation: Richard Hummel, Corina Sie, David I Watson, Tingting Wang, Alfiya Ansar, Damian J Hussey, Department of Surgery, Flinders University, Bedford Park, South Australia 5042, Australia.
authors.forename: Richard
authors.initials: R
authors.lastname: Hummel
authors.affiliation: Richard Hummel, Corina Sie, David I Watson, Tingting Wang, Alfiya Ansar, Damian J Hussey, Department of Surgery, Flinders University, Bedford Park, South Australia 5042, Australia.
authors.forename: Corina
authors.initials: C
authors.lastname: Sie
authors.affiliation: Richard Hummel, Corina Sie, David I Watson, Tingting Wang, Alfiya Ansar, Damian J Hussey, Department of Surgery, Flinders University, Bedford Park, South Australia 5042, Australia.
authors.forename: David I
authors.initials: DI
authors.lastname: Watson
authors.affiliation: Richard Hummel, Corina Sie, David I Watson, Tingting Wang, Alfiya Ansar, Damian J Hussey, Department of Surgery, Flinders University, Bedford Park, South Australia 5042, Australia.
authors.forename: Tingting
authors.initials: T
authors.lastname: Wang
authors.affiliation: Richard Hummel, Corina Sie, David I Watson, Tingting Wang, Alfiya Ansar, Damian J Hussey, Department of Surgery, Flinders University, Bedford Park, South Australia 5042, Australia.
authors.forename: Alfiya
authors.initials: A
authors.lastname: Ansar
authors.affiliation: Richard Hummel, Corina Sie, David I Watson, Tingting Wang, Alfiya Ansar, Damian J Hussey, Department of Surgery, Flinders University, Bedford Park, South Australia 5042, Australia.
authors.forename: Michael Z
authors.initials: MZ
authors.lastname: Michael
authors.affiliation: Richard Hummel, Corina Sie, David I Watson, Tingting Wang, Alfiya Ansar, Damian J Hussey, Department of Surgery, Flinders University, Bedford Park, South Australia 5042, Australia.
authors.forename: Mark
authors.initials: M
authors.lastname: Van der Hoek
authors.affiliation: Richard Hummel, Corina Sie, David I Watson, Tingting Wang, Alfiya Ansar, Damian J Hussey, Department of Surgery, Flinders University, Bedford Park, South Australia 5042, Australia.
authors.forename: Joerg
authors.initials: J
authors.lastname: Haier
authors.affiliation: Richard Hummel, Corina Sie, David I Watson, Tingting Wang, Alfiya Ansar, Damian J Hussey, Department of Surgery, Flinders University, Bedford Park, South Australia 5042, Australia.
authors.forename: Damian J
authors.initials: DJ
authors.lastname: Hussey
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D000970:Antineoplastic Agents / Q000494:pharmacology; D014408:Biomarkers, Tumor / Q000235:genetics* / Q000378:metabolism; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D002945:Cisplatin / Q000494:pharmacology; D019008:Drug Resistance, Neoplasm / Q000235:genetics*; D004938:Esophageal Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D000077277:Esophageal Squamous Cell Carcinoma; D005472:Fluorouracil / Q000494:pharmacology; D020869:Gene Expression Profiling / Q000379:methods; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D000970:Antineoplastic Agents; D014408:Biomarkers, Tumor; D035683:MicroRNAs; D002945:Cisplatin; D005472:Fluorouracil
keywords: Chemotherapy; Esophageal cancer; MicroRNA; Resistance; Target