title: Thioclava indica sp. nov., isolated from surface seawater of the Indian Ocean.
abstract: A short-rod shaped, non-motile and Gram-negative strain, designated as DT23-4(T), was isolated from a dotriacontane-degrading bacterial consortium, enriched in the surface seawater of the Indian Ocean. To delineate its taxonomic position, strain DT23-4(T) was subjected to polyphasic characterization. Strain DT23-4(T) can grow at temperatures from 10 to 41 °C, in the range of pH 6-10 and at salinities from 0 to 18 % (w/v). It is positive for denitrification, oxidase and catalase, but negative for gelatin hydrolysis and indole production. Phylogenetic analysis based on the 16S rRNA gene sequences indicated that strain DT23-4(T) affiliates to the genus Thioclava, sharing high sequence similarity to Thioclava dalianensis DLFJ1-1(T) (98.2 %), Thioclava atlantica 13D2W-2(T) (96.8 %), Thioclava pacifica TL 2(T) (95.9 %) and less than 95.7 % with other species of the family Rhodobacteraceae. The digital DNA:DNA hybridization (dDDH) pairwise values between strain DT23-4(T) and T. dalianensis DLFJ1-1(T), T. atlantica 13D2W-2(T) and T. pacifica TL 2(T) were 21.5 ± 2.3, 20.6 ± 2.3 and 20.9 ± 2.3 %, respectively. Similarly, the average nucleotide identity values between strain DT23-4(T) and these three type strains were 78.0, 77.1 and 77.3 %, respectively. The principal fatty acid was identified as Summed Feature 8 (C18:1 ω7c/ω6c) (81.9 %). The quinone of strain DT23-4(T) was identified as Q10 (100 %). The polar lipids of strain DT23-4(T) were found to contain phosphatidylethanolamine, phosphatidylglycerol, two glycolipids and four phospholipids. The genomic DNA G+C content was determined to be 60.3 mol%. On the basis of genotypic, phenotypic and chemotaxonomic analyses, strain DT23-4(T) can be concluded to represent a novel species of the genus Thioclava, for which the name Thioclava indica sp. nov. is proposed, with the type strain DT23-4(T) (= MCCC 1A00513(T) = LMG 27698(T) = KCTC 33533(T)).
authors.affiliation: State Key Laboratory Breeding Base of Marine Genetic Resources, Key Laboratory of Marine Genetic Resources, Third Institute of Oceanography, SOA, Collaborative Innovation Center of Deep Sea Biology, Key Laboratory of Marine Genetic Resources of Fujian Province, Xiamen, 361005, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.forename: Qiliang
authors.initials: Q
authors.lastname: Lai
authors.forename: Juan
authors.initials: J
authors.lastname: Du
authors.forename: Hongxiu
authors.initials: H
authors.lastname: Xu
authors.forename: Lijing
authors.initials: L
authors.lastname: Jiang
authors.forename: Zongze
authors.initials: Z
authors.lastname: Shao
mesh_terms: D000473:Alkanes / Q000378:metabolism; D015373:Bacterial Typing Techniques; D001482:Base Composition; D016000:Cluster Analysis; D003600:Cytosol / Q000737:chemistry; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D004798:Enzymes / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D016680:Genome, Bacterial; D006017:Glycolipids / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D019156:Indian Ocean; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D011809:Quinones / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D041902:Rhodobacteraceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D054712:Salinity; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D013696:Temperature
chemical_list: D000473:Alkanes; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D004798:Enzymes; D005227:Fatty Acids; D006017:Glycolipids; D010743:Phospholipids; D011809:Quinones; D012336:RNA, Ribosomal, 16S; C578748:dotriacontane