title: Rat brain normalization templates for robust regional analysis of [11C]ABP688 positron emission tomography/computed tomography.
abstract: A methodology to generate rat brain templates for spatial normalization of positron emission tomographic (PET)/computed tomographic (CT) images is described and applied to generate three different templates for imaging of [11C]ABP688, a PET ligand binding to the metabotropic glutamate 5 receptor. The templates are based on functional (PET), structural (CT), and combined PET and CT information, respectively. The templates are created from a test-retest study under normal conditions and are used to assess the different templates by using them in the analysis pipeline of a test-retest and a blocking experiment. The resulting average nondisplaceable binding potentials (BPND) show significant (analysis of variance, p < .05) and substantial (up to 23%) differences between the different approaches in several brain regions. The highest BPND values in receptor-rich regions are obtained using the PET-based approach. This approach also had the smallest variability in all tested regions (standard error of measurement of 9% versus 14% [PET/CT] and 20% [CT]). All approaches showed similar relative changes in BPND values with increased blocking. Taken together, these results suggest that the use of the tracer-specific PET-based template outperforms the other approaches with the performance of the combined PET/CT template between those of the PET and the tracer-independent CT template.
authors.forename: Jeroen
authors.initials: J
authors.lastname: Verhaeghe
authors.forename: Leonie
authors.initials: L
authors.lastname: Wyffels
authors.forename: Tine
authors.initials: T
authors.lastname: Wyckhuys
authors.forename: Sigrid
authors.initials: S
authors.lastname: Stroobants
authors.forename: Steven
authors.initials: S
authors.lastname: Staelens
mesh_terms: D000818:Animals; D001921:Brain / Q000000981:diagnostic imaging / Q000378:metabolism*; D064847:Multimodal Imaging; D010091:Oximes / Q000378:metabolism*; D049268:Positron-Emission Tomography; D011725:Pyridines / Q000378:metabolism*; D019275:Radiopharmaceuticals / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D064529:Receptor, Metabotropic Glutamate 5 / Q000378:metabolism; D014057:Tomography, X-Ray Computed
chemical_list: C510950:3-(6-methylpyridin-2-ylethynyl)cyclohex-2-enone-O-methyloxime; C578937:Grm5 protein, rat; D010091:Oximes; D011725:Pyridines; D019275:Radiopharmaceuticals; D064529:Receptor, Metabotropic Glutamate 5