title: Multiplex MALDI-MS imaging of plant metabolites using a hybrid MS system.
abstract: Plant tissues present intriguing systems for study by mass spectrometry imaging, as they exhibit a complex metabolism and a high degree of spatial localization. This chapter presents a methodology for preparation of plant tissue sections for matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) analysis and the use of a hybrid mass spectrometer for "multiplex" imaging. The multiplex method described here provides a wide range of analytical information, including high-resolution, accurate mass imaging and tandem MS scans for structural information, all within a single experiment. While this procedure was developed for plant tissues, it can be readily adapted for analysis of other sample types.
authors.affiliation: Department of Chemistry, Iowa State University of Science and Technology, Ames, IA, USA.
authors.forename: Andrew R
authors.initials: AR
authors.lastname: Korte
authors.forename: Gargey B
authors.initials: GB
authors.lastname: Yagnik
authors.forename: Adam D
authors.initials: AD
authors.lastname: Feenstra
authors.forename: Young Jin
authors.initials: YJ
authors.lastname: Lee
mesh_terms: D053000:Analytic Sample Preparation Methods; D003890:Desiccation; D057054:Molecular Imaging / Q000379:methods*; D010944:Plants / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D003313:Zea mays / Q000166:cytology / Q000378:metabolism