title: Dynamic piezoelectric stimulation enhances osteogenic differentiation of human adipose stem cells.
abstract: This work reports on the influence of the substrate polarization of electroactive β-poly(vinylidene fluoride) (β-PVDF) on human adipose stem cells (hASCs) differentiation under static and dynamic conditions. hASCs were cultured on different β-PVDF surfaces (non-poled and "poled -") adsorbed with fibronectin and osteogenic differentiation was determined using a quantitative alkaline phosphatase assay. "Poled -" β-PVDF samples promote higher osteogenic differentiation, which is even higher under dynamic conditions. It is thus demonstrated that electroactive membranes can provide the necessary electromechanical stimuli for the differentiation of specific cells and therefore will support the design of suitable tissue engineering strategies, such as bone tissue engineering.
authors.affiliation: Center/Department of Physics, University of Minho, Braga, 4710-057, Portugal; INL-International Iberian Nanotechnology Laboratory, 4715-330, Braga, Portugal.
authors.forename: Clarisse
authors.initials: C
authors.lastname: Ribeiro
authors.forename: Jenita
authors.initials: J
authors.lastname: Pärssinen
authors.forename: Vítor
authors.initials: V
authors.lastname: Sencadas
authors.forename: Vítor
authors.initials: V
authors.lastname: Correia
authors.forename: S
authors.initials: S
authors.lastname: Miettinen
authors.forename: Vesa P
authors.initials: VP
authors.lastname: Hytönen
authors.forename: Senentxu
authors.initials: S
authors.lastname: Lanceros-Méndez
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D004558:Electric Stimulation; D006801:Humans; D010012:Osteogenesis / Q000187:drug effects; D011145:Polyvinyls / Q000494:pharmacology; D013234:Stem Cells / Q000166:cytology*; D005465:Fluorocarbon Polymers
chemical_list: D011145:Polyvinyls; C024865:polyvinylidene fluoride; D005465:Fluorocarbon Polymers
keywords: dynamic conditions; electroactive polymer; osteogenesis; stem cell