title: Microbial composition and antibiotic resistance of biofilms recovered from endotracheal tubes of mechanically ventilated patients.
abstract: In critically ill patients, breathing is impaired and mechanical ventilation, using an endotracheal tube (ET) connected to a ventilator, is necessary. Although mechanical ventilation is a life-saving procedure, it is not without risk. Because of several reasons, a biofilm often forms at the distal end of the ET and this biofilm is a persistent source of bacteria which can infect the lungs, causing ventilator-associated pneumonia (VAP). There is a link between the microbial flora of ET biofilms and the microorganisms involved in the onset of VAP. Culture dependent and independent techniques were already used to identify the microbial flora of ET biofilms and also, the antibiotic resistance of microorganisms obtained from ET biofilms was determined. The ESKAPE pathogens play a dominant role in the onset of VAP and these organisms were frequently identified in ET biofilms. Also, antibiotic resistant microorganisms were frequently present in ET biofilms. Members of the normal oral flora were also identified in ET biofilms but it is thought that these organisms initiate ET biofilm formation and are not directly involved in the development of VAP.
authors.affiliation: Laboratory of Pharmaceutical Microbiology, Ghent University, Ottergemsesteenweg 460, 9000, Ghent, Belgium.
authors.forename: Ilse
authors.initials: I
authors.lastname: Vandecandelaere
authors.forename: Tom
authors.initials: T
authors.lastname: Coenye
mesh_terms: D000900:Anti-Bacterial Agents / Q000627:therapeutic use; D001419:Bacteria / Q000145:classification / Q000187:drug effects; D018441:Biofilms / Q000187:drug effects / Q000254:growth & development*; D002175:Candida / Q000145:classification / Q000187:drug effects; D004352:Drug Resistance, Microbial; D006801:Humans; D007442:Intubation, Intratracheal / Q000295:instrumentation*; D059013:Microbial Consortia / Q000187:drug effects / Q000502:physiology*; D053717:Pneumonia, Ventilator-Associated / Q000188:drug therapy / Q000382:microbiology; D012121:Respiration, Artificial / Q000295:instrumentation*
chemical_list: D000900:Anti-Bacterial Agents