title: Role of the DHH1 gene in the regulation of monocarboxylic acids transporters expression in Saccharomyces cerevisiae.
abstract: Previous experiments revealed that DHH1, a RNA helicase involved in the regulation of mRNA stability and translation, complemented the phenotype of a Saccharomyces cerevisiae mutant affected in the expression of genes coding for monocarboxylic-acids transporters, JEN1 and ADY2 (Paiva S, Althoff S, Casal M, Leao C. FEMS Microbiol Lett, 1999, 170:301-306). In wild type cells, JEN1 expression had been shown to be undetectable in the presence of glucose or formic acid, and induced in the presence of lactate. In this work, we show that JEN1 mRNA accumulates in a dhh1 mutant, when formic acid was used as sole carbon source. Dhh1 interacts with the decapping activator Dcp1 and with the deadenylase complex. This led to the hypothesis that JEN1 expression is post-transcriptionally regulated by Dhh1 in formic acid. Analyses of JEN1 mRNAs decay in wild-type and dhh1 mutant strains confirmed this hypothesis. In these conditions, the stabilized JEN1 mRNA was associated to polysomes but no Jen1 protein could be detected, either by measurable lactate carrier activity, Jen1-GFP fluorescence detection or western blots. These results revealed the complexity of the expression regulation of JEN1 in S. cerevisiae and evidenced the importance of DHH1 in this process. Additionally, microarray analyses of dhh1 mutant indicated that Dhh1 plays a large role in metabolic adaptation, suggesting that carbon source changes triggers a complex interplay between transcriptional and post-transcriptional effects.
authors.affiliation: Centre of Molecular and Environmental Biology (CBMA), Department of Biology, University of Minho, Campus de Gualtar, Braga, Portugal; Centre of Health and Environmental Research (CISA), School of Allied Health Sciences, Polytechnic Institute of Porto, Vila Nova de Gaia, Portugal.
authors.forename: Sandra
authors.initials: S
authors.lastname: Mota
authors.affiliation: Centre of Molecular and Environmental Biology (CBMA), Department of Biology, University of Minho, Campus de Gualtar, Braga, Portugal.
authors.forename: Neide
authors.initials: N
authors.lastname: Vieira
authors.affiliation: Centre of Molecular and Environmental Biology (CBMA), Department of Biology, University of Minho, Campus de Gualtar, Braga, Portugal.
authors.forename: Sónia
authors.initials: S
authors.lastname: Barbosa
authors.affiliation: Sorbonne Universités, Université Pierre et Marie Curie, UMR7238, Laboratoire de Biologie computationnelle et quantitative, Paris, France; CNRS, UMR7238, Laboratoire de Biologie computationnelle et quantitative, Paris, France.
authors.forename: Thierry
authors.initials: T
authors.lastname: Delaveau
authors.affiliation: CNRS, UMR8226, Laboratoire de Biologie Moléculaire et Cellulaire des Eucaryotes, Institut de Biologie Physico-Chimique, Paris, France; Sorbonne Universités, Université Pierre et Marie Curie UPMC, UMR8226, Laboratoire de Biologie Moléculaire et Cellulaire des Eucaryotes, Institut de Biologie Physico-Chimique, Paris, France.
authors.forename: Claire
authors.initials: C
authors.lastname: Torchet
authors.affiliation: CNRS, FRE3630, Laboratoire de l'Expression Génétique Microbienne, Institut de Biologie Physico-Chimique, Paris, France.
authors.forename: Agnès
authors.initials: A
authors.lastname: Le Saux
authors.affiliation: Sorbonne Universités, Université Pierre et Marie Curie, UMR7238, Laboratoire de Biologie computationnelle et quantitative, Paris, France; CNRS, UMR7238, Laboratoire de Biologie computationnelle et quantitative, Paris, France.
authors.forename: Mathilde
authors.initials: M
authors.lastname: Garcia
authors.affiliation: Centre of Molecular and Environmental Biology (CBMA), Department of Biology, University of Minho, Campus de Gualtar, Braga, Portugal.
authors.forename: Ana
authors.initials: A
authors.lastname: Pereira
authors.affiliation: École normale supérieure, Institut de Biologie de l'ENS, IBENS, Paris, France; Inserm, U1024, Paris, France; CNRS, UMR 8197, Paris, France.
authors.forename: Sophie
authors.initials: S
authors.lastname: Lemoine
authors.affiliation: École normale supérieure, Institut de Biologie de l'ENS, IBENS, Paris, France; Inserm, U1024, Paris, France; CNRS, UMR 8197, Paris, France.
authors.forename: Fanny
authors.initials: F
authors.lastname: Coulpier
authors.affiliation: École normale supérieure, Institut de Biologie de l'ENS, IBENS, Paris, France; Inserm, U1024, Paris, France; CNRS, UMR 8197, Paris, France.
authors.forename: Xavier
authors.initials: X
authors.lastname: Darzacq
authors.affiliation: CNRS, UMR8226, Laboratoire de Biologie Moléculaire et Cellulaire des Eucaryotes, Institut de Biologie Physico-Chimique, Paris, France; Sorbonne Universités, Université Pierre et Marie Curie UPMC, UMR8226, Laboratoire de Biologie Moléculaire et Cellulaire des Eucaryotes, Institut de Biologie Physico-Chimique, Paris, France.
authors.forename: Lionel
authors.initials: L
authors.lastname: Benard
authors.affiliation: Centre of Molecular and Environmental Biology (CBMA), Department of Biology, University of Minho, Campus de Gualtar, Braga, Portugal.
authors.forename: Margarida
authors.initials: M
authors.lastname: Casal
authors.affiliation: Sorbonne Universités, Université Pierre et Marie Curie, UMR7238, Laboratoire de Biologie computationnelle et quantitative, Paris, France; CNRS, UMR7238, Laboratoire de Biologie computationnelle et quantitative, Paris, France.
authors.forename: Frédéric
authors.initials: F
authors.lastname: Devaux
authors.affiliation: Centre of Molecular and Environmental Biology (CBMA), Department of Biology, University of Minho, Campus de Gualtar, Braga, Portugal.
authors.forename: Sandra
authors.initials: S
authors.lastname: Paiva
mesh_terms: D000222:Adaptation, Physiological; D053487:DEAD-box RNA Helicases / Q000235:genetics* / Q000378:metabolism*; D005561:Formates / Q000378:metabolism; D020869:Gene Expression Profiling; D015966:Gene Expression Regulation, Fungal; D055106:Genome-Wide Association Study; D027501:Monocarboxylic Acid Transporters / Q000235:genetics* / Q000378:metabolism; D009154:Mutation; D011132:Polyribosomes / Q000378:metabolism; D020871:RNA Stability; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics* / Q000378:metabolism*; D027981:Symporters / Q000235:genetics / Q000378:metabolism
chemical_list: D005561:Formates; C118697:JEN1 protein, S cerevisiae; D027501:Monocarboxylic Acid Transporters; D012333:RNA, Messenger; D029701:Saccharomyces cerevisiae Proteins; D027981:Symporters; C030544:formic acid; C445083:DHH1 protein, S cerevisiae; D053487:DEAD-box RNA Helicases