title: Solution NMR of MPS-1 reveals a random coil cytosolic domain structure.
abstract: Caenorhabditis elegans MPS1 is a single transmembrane helical auxiliary subunit that co-localizes with the voltage-gated potassium channel KVS1 in the nematode nervous system. MPS-1 shares high homology with KCNE (potassium voltage-gated channel subfamily E member) auxiliary subunits, and its cytosolic domain was reported to have a serine/threonine kinase activity that modulates KVS1 channel function via phosphorylation. In this study, NMR spectroscopy indicated that the full length and truncated MPS-1 cytosolic domain (134-256) in the presence or absence of n-dodecylphosphocholine detergent micelles adopted a highly flexible random coil secondary structure. In contrast, protein kinases usually adopt a stable folded conformation in order to implement substrate recognition and phosphoryl transfer. The highly flexible random coil secondary structure suggests that MPS-1 in the free state is unstructured but may require a substrate or binding partner to adopt stable structure required for serine/threonine kinase activity.
authors.affiliation: Hefei National Laboratory of Microscale Physical Sciences, School of Life Sciences, University of Science and Technology of China, Hefei, Anhui, P. R. China.
authors.forename: Pan
authors.initials: P
authors.lastname: Li
authors.affiliation: High Magnetic Field Laboratory, Hefei institutes of Physical Science, Chinese Academy of Sciences, Hefei, Anhui, P. R. China.
authors.forename: Pan
authors.initials: P
authors.lastname: Shi
authors.affiliation: Hefei National Laboratory of Microscale Physical Sciences, School of Life Sciences, University of Science and Technology of China, Hefei, Anhui, P. R. China.
authors.forename: Chaohua
authors.initials: C
authors.lastname: Lai
authors.affiliation: Hefei National Laboratory of Microscale Physical Sciences, School of Life Sciences, University of Science and Technology of China, Hefei, Anhui, P. R. China.
authors.forename: Juan
authors.initials: J
authors.lastname: Li
authors.affiliation: Hefei National Laboratory of Microscale Physical Sciences, School of Life Sciences, University of Science and Technology of China, Hefei, Anhui, P. R. China.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Zheng
authors.affiliation: Hefei National Laboratory of Microscale Physical Sciences, School of Life Sciences, University of Science and Technology of China, Hefei, Anhui, P. R. China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Xiong
authors.affiliation: Hefei National Laboratory of Microscale Physical Sciences, School of Life Sciences, University of Science and Technology of China, Hefei, Anhui, P. R. China.
authors.forename: Longhua
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Hefei National Laboratory of Microscale Physical Sciences, School of Life Sciences, University of Science and Technology of China, Hefei, Anhui, P. R. China; High Magnetic Field Laboratory, Hefei institutes of Physical Science, Chinese Academy of Sciences, Hefei, Anhui, P. R. China.
authors.forename: Changlin
authors.initials: C
authors.lastname: Tian
mesh_terms: D000818:Animals; D029742:Caenorhabditis elegans Proteins / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008823:Micelles; D019906:Nuclear Magnetic Resonance, Biomolecular; D010455:Peptides / Q000737:chemistry; D010767:Phosphorylcholine / Q000031:analogs & derivatives / Q000737:chemistry; D015221:Potassium Channels / Q000737:chemistry*; D011487:Protein Conformation; D054730:Protein Interaction Domains and Motifs; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D012996:Solutions
chemical_list: D029742:Caenorhabditis elegans Proteins; C473893:MPS-1 protein, C elegans; D008823:Micelles; D010455:Peptides; D015221:Potassium Channels; D011994:Recombinant Proteins; D012996:Solutions; D010767:Phosphorylcholine; C028810:dodecylphosphocholine