title: [Comparison and analysis of wild and cultivated Lonicerae japonicae flos from Xinmi].
abstract: OBJECTIVE
  To compare and analyze the wild and cultivated Lonicerae Japonicae Flos from Xinmi.
  
  
  METHODS
  Macroscopic identification and microscopic identification were adopted to compare and analyze characteristics of the wild and cultivated Lonicerae Japonicae Flos from Xinmi. The content of chlorogenic acid and luteoloside were determined by the methods in the Chinese Pharmacopoeia. DNA molecular marker technology was used to identify Lonicerae Japonicae Flos of different varieties.
  
  
  RESULTS
  The differences in macroscopic and microscopic characteristics as well as the content of chlorogenic acid and luteoloside between them had been found. PCR amplification reaction system and procedure of Lonicerae Japonicae Flos from Xinmi were optimized. An effective primer for identification was chosen from 11 primers.
  
  
  CONCLUSIONS
  The results provide the scientific basis for quality assessment of Lonicerae Japonicae Flos from Xinmi.
authors.forename: Sui-Qing
authors.initials: SQ
authors.lastname: Chen
authors.forename: Xiao-Ya
authors.initials: XY
authors.lastname: Sun
authors.forename: Xiao-Jun
authors.initials: XJ
authors.lastname: Liu
authors.forename: Xiao
authors.initials: X
authors.lastname: Dai
mesh_terms: D002726:Chlorogenic Acid / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D017931:DNA Primers / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000235:genetics*; D035264:Flowers / Q000033:anatomy & histology* / Q000145:classification / Q000235:genetics; D031239:Lonicera / Q000033:anatomy & histology* / Q000145:classification / Q000235:genetics*; D008854:Microscopy, Electron; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011786:Quality Control; D017422:Sequence Analysis, DNA
chemical_list: D017931:DNA Primers; D018744:DNA, Plant; D021903:DNA, Ribosomal Spacer; D002726:Chlorogenic Acid