title: [Chromosomal translocation involving USP6 gene in nodular fasciitis].
abstract: OBJECTIVE
  To investigate the frequency of USP6 gene rearrangement in nodular fasciitis (NF) and to evaluate its clinical application.
  
  
  METHODS
  Twenty nine cases of previously diagnosed NF were screened for the presence of the USP6 gene rearrangement by interphase fluorescence-in-situ hybridization (FISH) on formalin-fixed paraffin-embedded tissue. Fifteen of these cases, which had available tissue, were also analysed for MYH9-USP6 fusion transcripts by reverse transcription-polymerase chain reaction (RT-PCR).
  
  
  RESULTS
  Twenty four of the 29 cases (83%) were positive for the USP6 gene rearrangement by interphase FISH. The 15 cases with RT-PCR showed the following results: 11 positive, one deletion and three negative for USP6 gene rearrangement. Of these 15 cases, eight (8/15) showed MYH9-USP6 fusion transcript by RT-PCR. Of these eight cases, seven were positive for USP6 gene rearrangement and one showed USP6 deletion by FISH.
  
  
  CONCLUSIONS
  USP6 gene rearrangement is a recurrent genetic event in NF. It is a valuable ancillary tool for the pathological diagnosis of these lesions.
authors.affiliation: Department of Pathology, the Affiliated Tumor Hospital of Guangxi Medical University, Nanning 530021, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Chen
authors.forename: Xinqing
authors.initials: X
authors.lastname: Ye
authors.forename: Yao
authors.initials: Y
authors.lastname: Li
authors.forename: Changhong
authors.initials: C
authors.lastname: Wei
authors.forename: Qian
authors.initials: Q
authors.lastname: Zheng
authors.forename: Ping
authors.initials: P
authors.lastname: Zhong
authors.forename: Shengming
authors.initials: S
authors.lastname: Wu
authors.forename: Yuan
authors.initials: Y
authors.lastname: Luo
authors.forename: Zhiling
authors.initials: Z
authors.lastname: Liao
authors.affiliation: E-mail: dr.hongtaoye@googlemail.com.
authors.forename: Hongtao
authors.initials: H
authors.lastname: Ye
mesh_terms: D005208:Fasciitis / Q000235:genetics*; D015321:Gene Rearrangement; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007399:Interphase; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014178:Translocation, Genetic; D043222:Ubiquitin Thiolesterase / Q000235:genetics*
chemical_list: D011518:Proto-Oncogene Proteins; C473330:USP6 protein, human; D043222:Ubiquitin Thiolesterase