title: Effect of amphiphilic PCL-PEG nano-micelles on HepG2 cell migration.
abstract: Uptake of nanoparticles (NPs) affects cell migration but the mechanism remains poorly understood. In this study, the amphiphilic block PCL-PEG nano-micelles with well-controlled hydrophilic/hydrophobic chains were used to investigate the effect of internalized nano-micelles on cancer cell migration. Our results indicated that the nano-micelles with medium PCL and PEG chains increased expression of Rho GTPases and impeded focal adhesion components. This could enhance Hep G2 cell motility. The nano-micelles with large PCL and PEG chains showed lower Rho GTPase levels and higher FA components. This is consistent with slower cell migration. Understanding the mechanism of NPs regulating cell behaviors may help the design of efficient drug delivery systems based on polymer micelles.
authors.affiliation: Institute of Biomedical Engineering, School of Preclinical and Forensic Medicine, Sichuan University, Chengdu, 610041, P. R. China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Shen
authors.forename: Mengtian
authors.initials: M
authors.lastname: Leng
authors.forename: Hongchi
authors.initials: H
authors.lastname: Yu
authors.forename: Qin
authors.initials: Q
authors.lastname: Zhang
authors.forename: Xianglin
authors.initials: X
authors.lastname: Luo
authors.forename: Hans
authors.initials: H
authors.lastname: Gregersen
authors.forename: Guixue
authors.initials: G
authors.lastname: Wang
authors.forename: Xiaoheng
authors.initials: X
authors.lastname: Liu
mesh_terms: D002465:Cell Movement / Q000187:drug effects*; D004705:Endocytosis / Q000187:drug effects; D005027:Ethylene Oxide / Q000494:pharmacology*; D051416:Focal Adhesion Protein-Tyrosine Kinases / Q000378:metabolism; D056945:Hep G2 Cells; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D016023:Integrins / Q000378:metabolism; D007783:Lactones / Q000494:pharmacology*; D008823:Micelles; D046529:Microscopy, Electron, Transmission; D008970:Molecular Weight; D053758:Nanoparticles / Q000737:chemistry*; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism; D010766:Phosphorylation / Q000187:drug effects; D021122:Protein Subunits / Q000378:metabolism; D020741:rho GTP-Binding Proteins / Q000378:metabolism
chemical_list: D016023:Integrins; D007783:Lactones; D008823:Micelles; C400421:PLC(20)-b-PEO(44); D021122:Protein Subunits; D019869:Phosphatidylinositol 3-Kinases; D051416:Focal Adhesion Protein-Tyrosine Kinases; D020741:rho GTP-Binding Proteins; D005027:Ethylene Oxide
keywords: PCL-PEG; Rho GTPases; amphiphiles; biodegradable; cell migration