title: PKCδ and θ possibly mediate FSH-induced mouse oocyte maturation via NOX-ROS-TACE cascade signaling pathway.
abstract: In mammals, gonadotropins stimulate oocyte maturation via the epidermal growth factor (EGF) network, and the protein kinase C (PKC) signaling pathway mediates this process. Tumor necrosis factor-α converting enzyme (TACE) is an important protein responding to PKC activation. However, the detailed signaling cascade between PKC and TACE in follicle-stimulating hormone (FSH)-induced oocyte maturation in vitro remains unclear. In this study, we found that rottlerin (mallotoxin, MTX), the inhibitor of PKC δ and θ, blocked FSH-induced maturation of mouse cumulus-oocyte complexes (COCs) in vitro. We further clarified the relationship between two molecules downstream of PKC δ and θ and TACE in COCs: nicotinamide adenine dinucleotide phosphate (NADPH) oxidase (NOX) and its products, reactive oxygen species (ROS). We proved that the respective inhibitors of NOX, ROS and TACE could block FSH-stimulated oocyte maturation dose-dependently, but these inhibitory effects could be reversed partially by amphiregulin (Areg), an EGF family member. Notably, inhibition of PKC δ and θ prevented FSH-induced translocation of two cytosolic components of NOX, p47phox and p67phox, to the plasma membrane in cumulus cells. Moreover, FSH-induced TACE activity in cumulus cells was decreased markedly by inhibition of NOX and ROS. In conclusion, PKC δ and θ possibly mediate FSH-induced meiotic resumption in mouse COCs via NOX-ROS-TACE signaling pathway.
authors.affiliation: State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing, People's Republic of China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing, People's Republic of China.
authors.forename: Wenqiang
authors.initials: W
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing, People's Republic of China.
authors.forename: Hao
authors.initials: H
authors.lastname: Ran
authors.affiliation: State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing, People's Republic of China.
authors.forename: Lizhao
authors.initials: L
authors.lastname: Feng
authors.affiliation: State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing, People's Republic of China.
authors.forename: Hao
authors.initials: H
authors.lastname: Yan
authors.affiliation: Department of histology and embryology, Chongqing medical university, Chongqing, People's Republic of China.
authors.forename: Xinyi
authors.initials: X
authors.lastname: Mu
authors.affiliation: College of biological sciences and technology, Beijing Forestry University, Beijing, People's Republic of China.
authors.forename: Yingying
authors.initials: Y
authors.lastname: Han
authors.affiliation: State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing, People's Republic of China.
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing, People's Republic of China.
authors.forename: Guoliang
authors.initials: G
authors.lastname: Xia
authors.affiliation: State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing, People's Republic of China.
authors.forename: Chao
authors.initials: C
authors.lastname: Wang
mesh_terms: D051722:ADAM Proteins / Q000378:metabolism*; D000072198:ADAM17 Protein; D000098:Acetophenones / Q000737:chemistry; D066258:Amphiregulin / Q000378:metabolism; D000818:Animals; D001578:Benzopyrans / Q000737:chemistry; D001835:Body Weight; D002478:Cells, Cultured; D005260:Female; D005640:Follicle Stimulating Hormone / Q000378:metabolism*; D007527:Isoenzymes / Q000378:metabolism*; D008540:Meiosis; D051379:Mice; D008856:Microscopy, Fluorescence; D009247:NADH, NADPH Oxidoreductases / Q000378:metabolism; D000074624:NADPH Oxidase 1; D009865:Oocytes / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D010587:Phagocytosis; D011493:Protein Kinase C / Q000378:metabolism*; D051745:Protein Kinase C-delta / Q000378:metabolism*; D000074121:Protein Kinase C-theta; D017382:Reactive Oxygen Species / Q000378:metabolism; D015398:Signal Transduction
chemical_list: D000098:Acetophenones; D066258:Amphiregulin; D001578:Benzopyrans; D007527:Isoenzymes; D017382:Reactive Oxygen Species; D005640:Follicle Stimulating Hormone; C085746:rottlerin; D009247:NADH, NADPH Oxidoreductases; D000074624:NADPH Oxidase 1; C489593:Prkcq protein, mouse; D011493:Protein Kinase C; D051745:Protein Kinase C-delta; D000074121:Protein Kinase C-theta; D051722:ADAM Proteins; D000072198:ADAM17 Protein; C000606463:Adam17 protein, mouse