title: A novel peptide interferes with Mycobacterium tuberculosis virulence and survival.
abstract: Tuberculosis (TB) is a huge global burden, with new and resistant strains emerging at an alarming rate, necessitating an urgent need for a new class of drug candidates. Here, we report that SL3, a novel 33-amino acid peptide, causes debilitating effects on mycobacterial morphology. Treatment with SL3 drastically inhibits the growth of Mycobacterium tuberculosis in vitro as well as in a pre-clinical mouse model for M.tb infection. Microarray analysis of SL3-expressing strain demonstrates wide-scale transcriptional disruption in M.tb. We therefore believe that SL3 and similar peptides may herald a new approach towards discovering new molecules for TB therapy.
authors.affiliation: Recombinant Gene Products Group, International Centre for Genetic Engineering and Biotechnology, ICGEB, Aruna Asaf Ali Marg, New Delhi 110067, India.
authors.forename: Sachin Kumar
authors.initials: SK
authors.lastname: Samuchiwal
authors.affiliation: Immunology Group, International Centre for Genetic Engineering and Biotechnology, ICGEB, Aruna Asaf Ali Marg, New Delhi 110067, India ; Department of Biochemistry, University of Calcutta, 35, Ballygunge Circular Road, Kolkata 700 019, India ; School of Laboratory Medicine, College of Health Sciences, University of Kwazulu-Natal, Durban, South Africa.
authors.forename: Sultan
authors.initials: S
authors.lastname: Tousif
authors.affiliation: Immunology Group, International Centre for Genetic Engineering and Biotechnology, ICGEB, Aruna Asaf Ali Marg, New Delhi 110067, India.
authors.forename: Dhiraj Kumar
authors.initials: DK
authors.lastname: Singh
authors.affiliation: Immunology Group, International Centre for Genetic Engineering and Biotechnology, ICGEB, Aruna Asaf Ali Marg, New Delhi 110067, India.
authors.forename: Arun
authors.initials: A
authors.lastname: Kumar
authors.affiliation: Recombinant Gene Products Group, International Centre for Genetic Engineering and Biotechnology, ICGEB, Aruna Asaf Ali Marg, New Delhi 110067, India.
authors.forename: Anamika
authors.initials: A
authors.lastname: Ghosh
authors.affiliation: Recombinant Gene Products Group, International Centre for Genetic Engineering and Biotechnology, ICGEB, Aruna Asaf Ali Marg, New Delhi 110067, India.
authors.forename: Kuhulika
authors.initials: K
authors.lastname: Bhalla
authors.affiliation: Recombinant Gene Products Group, International Centre for Genetic Engineering and Biotechnology, ICGEB, Aruna Asaf Ali Marg, New Delhi 110067, India.
authors.forename: Prem
authors.initials: P
authors.lastname: Prakash
authors.affiliation: Recombinant Gene Products Group, International Centre for Genetic Engineering and Biotechnology, ICGEB, Aruna Asaf Ali Marg, New Delhi 110067, India.
authors.forename: Sushil
authors.initials: S
authors.lastname: Kumar
authors.affiliation: Innovative Life Discoveries Pvt. Ltd., IMT Manesar, Haryana, India.
authors.forename: Ashish Chandra
authors.initials: AC
authors.lastname: Trivedi
authors.affiliation: Department of Biochemistry, University of Calcutta, 35, Ballygunge Circular Road, Kolkata 700 019, India.
authors.forename: Maitree
authors.initials: M
authors.lastname: Bhattacharyya
authors.affiliation: School of Laboratory Medicine, College of Health Sciences, University of Kwazulu-Natal, Durban, South Africa.
authors.forename: Gobardhan
authors.initials: G
authors.lastname: Das
authors.affiliation: Recombinant Gene Products Group, International Centre for Genetic Engineering and Biotechnology, ICGEB, Aruna Asaf Ali Marg, New Delhi 110067, India.
authors.forename: Anand
authors.initials: A
authors.lastname: Ranganathan
keywords: AMADID, agilent microarray design identifiers; Antimycobacterial peptides; CFU, colony forming units; ESAT6; MOI, multiplicity of infection; Mycobacterium tuberculosis; Protein–protein interaction; RD1, region of difference 1