title: Identification of best indicators of peptide-spectrum match using a permutation resampling approach.
abstract: Various indicators of observed-theoretical spectrum matches were compared and the resulting statistical significance was characterized using permutation resampling. Novel decoy databases built by resampling the terminal positions of peptide sequences were evaluated to identify the conditions for accurate computation of peptide match significance levels. The methodology was tested on real and manually curated tandem mass spectra from peptides across a wide range of sizes. Spectra match indicators from complementary database search programs were profiled and optimal indicators were identified. The combination of the optimal indicator and permuted decoy databases improved the calculation of the peptide match significance compared to the approaches currently implemented in the database search programs that rely on distributional assumptions. Permutation tests using p-values obtained from software-dependent matching scores and E-values outperformed permutation tests using all other indicators. The higher overlap in matches between the database search programs when using end permutation compared to existing approaches confirmed the superiority of the end permutation method to identify peptides. The combination of effective match indicators and the end permutation method is recommended for accurate detection of peptides.
authors.affiliation: Department of Animal Sciences, University of Illinois Urbana-Champaign, Urbana, IL 61801, USA.
authors.forename: Malik N
authors.initials: MN
authors.lastname: Akhtar
authors.forename: Bruce R
authors.initials: BR
authors.lastname: Southey
authors.forename: Per E
authors.initials: PE
authors.lastname: Andrén
authors.forename: Jonathan V
authors.initials: JV
authors.lastname: Sweedler
authors.forename: Sandra L
authors.initials: SL
authors.lastname: Rodriguez-Zas
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D019295:Computational Biology; D030562:Databases, Protein; D051379:Mice; D010455:Peptides / Q000737:chemistry*; D040901:Proteomics; D012984:Software; D053719:Tandem Mass Spectrometry
chemical_list: D010455:Peptides
keywords: Ends permuted decoy database; Neuropeptides; database search programs; p-value; permutations; significance levels; tandem MS