title: Phospholipase D2 is involved in the formation of Golgi tubules and ArfGAP1 recruitment.
abstract: Lipids and lipid-modifying enzymes play a key role in the biogenesis, maintenance and fission of transport carriers in the secretory and endocytic pathways. In the present study we demonstrate that phosphatidic acid generated by phospholipase D2 (PLD2) is involved in the formation of Golgi tubules. The main evidence to support this is: 1) inhibitors of phosphatidic acid formation and PLD2 depletion inhibit the formation of tubules containing resident enzymes and regulators of intra-Golgi transport in a low temperature (15°C) model of Golgi tubulation but do not affect brefeldin A-induced tubules, 2) inhibition of PLD2 enzymatic activity and PLD2 depletion in cells cultured under physiological conditions (37°C) induce the formation of tubules specifically containing Golgi matrix proteins, and, 3) over-expression of PLD2 induces the formation of a tubular network. In addition, it was found that the generation of this lipid by the isoenzyme is necessary for ArfGAP1 recruitment to Golgi membranes. These results suggest that both proteins are involved in the molecular mechanisms which drive the formation of different types of Golgi tubules.
authors.affiliation: Department of Cell Biology and Histology, Medical School, IMIB-Arrixaca, University of Murcia, Murcia, Spain.
authors.forename: Narcisa
authors.initials: N
authors.lastname: Martínez-Martínez
authors.affiliation: Department of Cell Biology and Histology, Medical School, IMIB-Arrixaca, University of Murcia, Murcia, Spain.
authors.forename: Emma
authors.initials: E
authors.lastname: Martínez-Alonso
authors.affiliation: Department of Cell Biology and Histology, Medical School, IMIB-Arrixaca, University of Murcia, Murcia, Spain.
authors.forename: José
authors.initials: J
authors.lastname: Ballesta
authors.affiliation: Department of Cell Biology and Histology, Medical School, IMIB-Arrixaca, University of Murcia, Murcia, Spain.
authors.forename: José A
authors.initials: JA
authors.lastname: Martínez-Menárguez
mesh_terms: D020690:GTPase-Activating Proteins / Q000235:genetics / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D010712:Phosphatidic Acids / Q000378:metabolism; D010739:Phospholipase D / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D021381:Protein Transport; D021601:trans-Golgi Network / Q000378:metabolism*
chemical_list: C503237:ARFGAP1 protein, human; D020690:GTPase-Activating Proteins; D010712:Phosphatidic Acids; C105606:phospholipase D2; D010739:Phospholipase D