title: Generation of a neuro-specific microarray reveals novel differentially expressed noncoding RNAs in mouse models for neurodegenerative diseases.
abstract: We have generated a novel, neuro-specific ncRNA microarray, covering 1472 ncRNA species, to investigate their expression in different mouse models for central nervous system diseases. Thereby, we analyzed ncRNA expression in two mouse models with impaired calcium channel activity, implicated in Epilepsy or Parkinson's disease, respectively, as well as in a mouse model mimicking pathophysiological aspects of Alzheimer's disease. We identified well over a hundred differentially expressed ncRNAs, either from known classes of ncRNAs, such as miRNAs or snoRNAs or which represented entirely novel ncRNA species. Several differentially expressed ncRNAs in the calcium channel mouse models were assigned as miRNAs and target genes involved in calcium signaling, thus suggesting feedback regulation of miRNAs by calcium signaling. In the Alzheimer mouse model, we identified two snoRNAs, whose expression was deregulated prior to amyloid plaque formation. Interestingly, the presence of snoRNAs could be detected in cerebral spine fluid samples in humans, thus potentially serving as early diagnostic markers for Alzheimer's disease. In addition to known ncRNAs species, we also identified 63 differentially expressed, entirely novel ncRNA candidates, located in intronic or intergenic regions of the mouse genome, genomic locations, which previously have been shown to harbor the majority of functional ncRNAs.
authors.affiliation: Division of Genomics and RNomics, Innsbruck Biocenter, Medical University of Innsbruck, 6020 Innsbruck, Austria.
authors.forename: Ronald
authors.initials: R
authors.lastname: Gstir
authors.affiliation: Division of Genomics and RNomics, Innsbruck Biocenter, Medical University of Innsbruck, 6020 Innsbruck, Austria.
authors.forename: Simon
authors.initials: S
authors.lastname: Schafferer
authors.affiliation: RNA Biology Group, Institute for Genomics and Bioinformatics, Graz University of Technology, 8010 Graz, Austria.
authors.forename: Marcel
authors.initials: M
authors.lastname: Scheideler
authors.affiliation: Division of Genomics and RNomics, Innsbruck Biocenter, Medical University of Innsbruck, 6020 Innsbruck, Austria.
authors.forename: Matthias
authors.initials: M
authors.lastname: Misslinger
authors.affiliation: Division of Genomics and RNomics, Innsbruck Biocenter, Medical University of Innsbruck, 6020 Innsbruck, Austria.
authors.forename: Matthias
authors.initials: M
authors.lastname: Griehl
authors.affiliation: Department of Psychiatry and Psychotherapy, University Clinic of General and Social Psychiatry, Innsbruck Medical University, 6020 Innsbruck, Austria.
authors.forename: Nina
authors.initials: N
authors.lastname: Daschil
authors.affiliation: Department of Psychiatry and Psychotherapy, University Clinic of General and Social Psychiatry, Innsbruck Medical University, 6020 Innsbruck, Austria.
authors.forename: Christian
authors.initials: C
authors.lastname: Humpel
authors.affiliation: Division of Physiology, Department of Physiology and Medical Physics, Innsbruck Medical University, 6020 Innsbruck, Austria.
authors.forename: Gerald J
authors.initials: GJ
authors.lastname: Obermair
authors.affiliation: Pharmacology and Toxicology, Institute of Pharmacy, and Center for Molecular Biosciences, University of Innsbruck, 6020 Innsbruck, Austria.
authors.forename: Claudia
authors.initials: C
authors.lastname: Schmuckermair
authors.affiliation: Pharmacology and Toxicology, Institute of Pharmacy, and Center for Molecular Biosciences, University of Innsbruck, 6020 Innsbruck, Austria.
authors.forename: Joerg
authors.initials: J
authors.lastname: Striessnig
authors.affiliation: Division of Physiology, Department of Physiology and Medical Physics, Innsbruck Medical University, 6020 Innsbruck, Austria.
authors.forename: Bernhard E
authors.initials: BE
authors.lastname: Flucher
authors.affiliation: Division of Genomics and RNomics, Innsbruck Biocenter, Medical University of Innsbruck, 6020 Innsbruck, Austria alexander.huettenhofer@i-med.ac.at.
authors.forename: Alexander
authors.initials: A
authors.lastname: Hüttenhofer
mesh_terms: D000544:Alzheimer Disease / Q000235:genetics* / Q000473:pathology; D000818:Animals; D015220:Calcium Channels / Q000235:genetics; D002490:Central Nervous System / Q000378:metabolism / Q000473:pathology; D004195:Disease Models, Animal; D004827:Epilepsy / Q000235:genetics* / Q000473:pathology; D005786:Gene Expression Regulation; D016678:Genome; D006801:Humans; D051379:Mice; D035683:MicroRNAs / Q000096:biosynthesis* / Q000235:genetics; D009928:Organ Specificity; D010300:Parkinson Disease / Q000235:genetics* / Q000473:pathology; D022661:RNA, Untranslated / Q000096:biosynthesis* / Q000235:genetics; D046888:Tissue Array Analysis
chemical_list: D015220:Calcium Channels; D035683:MicroRNAs; D022661:RNA, Untranslated
keywords: Alzheimer's disease; Parkinson's disease; microarray; noncoding RNAs; snoRNA; voltage-gated Ca2+ channels