title: Role of CD11b/CD18 in priming of human leukocytes by endotoxin glycoforms from Escherichia coli.
abstract: The primary objective of this study was to determine the role of β2 integrin α-subunit (CD11b) in the mechanism of human polymorphonuclear leukocyte (PML) priming by S or Re endotoxin glycoforms from Escherichia coli for fMLP-induced respiratory burst. Similar priming activity of S and Re endotoxin glycoforms for fMLP-induced reactive oxygen species (ROS) generation from primed PML was found. Anti-CD11b antibodies (clone ICRF 44) as well as isotype-matched immunoglobulin G1 (clone MOPC-21) do not influence the fMLP-induced ROS generation from unprimed PML. Antibodies against CD11b do not change fMLP-induced ROS generation from endotoxin-primed PML as well. The involvement of different isoforms of Fcγ receptors in fMLP-induced ROS generation from activated PML is proposed.
authors.affiliation: Institute of Basic Biological Problems, Russian Academy of Sciences, Pushchino, Moscow Region, 142290, Russia. kabanovd1@rambler.ru.
authors.forename: D S
authors.initials: DS
authors.lastname: Kabanov
authors.forename: S V
authors.initials: SV
authors.lastname: Grachev
authors.forename: I R
authors.initials: IR
authors.lastname: Prokhorenko
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology; D039481:CD11b Antigen / Q000276:immunology*; D018821:CD18 Antigens / Q000276:immunology*; D004731:Endotoxins / Q000737:chemistry* / Q000276:immunology*; D004926:Escherichia coli / Q000737:chemistry* / Q000276:immunology*; D006031:Glycosylation; D006801:Humans; D007962:Leukocytes / Q000166:cytology / Q000276:immunology*; D008070:Lipopolysaccharides / Q000276:immunology; D017382:Reactive Oxygen Species / Q000378:metabolism; D016897:Respiratory Burst / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D039481:CD11b Antigen; D018821:CD18 Antigens; D004731:Endotoxins; D008070:Lipopolysaccharides; D017382:Reactive Oxygen Species