title: Determination of Total Glutathione in Dried Blood Spot Samples Using a High-Performance Liquid Chromatography.
abstract: A method is described for the determination of total glutathione (TGSH) in dried blood spot (DBS) samples using high-performance liquid chromatography with fluorescence detection. Whole blood and DBS samples were obtained from a group of blood donors. After GSH reduction with dithiothreitol and protein precipitation with ethanol, the samples were derivatized with naphthalene-2,3-dicarboxaldehyde to form a very stable, highly fluorescent derivative. For the separation, a reversed phase HPLC method was used. The mixture of ethanol and deionized water (8 : 92, v/v) was used as a mobile phase. The analytical performance of this method was satisfactory: the intra- and interassay coefficients of variation were below 10%. Quantitative recoveries from spiked DBS samples were between 98.3 and 103.6%. The presented method is inexpensive and suitable for clinical testing purposes.
authors.affiliation: Department of Biological and Biochemical Sciences, Faculty of Chemical Technology, University of Pardubice, Studentska 573, 532 10 Pardubice, Czech Republic roman.kandar@upce.cz.
authors.forename: Roman
authors.initials: R
authors.lastname: Kanďár
authors.affiliation: Department of Biological and Biochemical Sciences, Faculty of Chemical Technology, University of Pardubice, Studentska 573, 532 10 Pardubice, Czech Republic.
authors.forename: Xenie
authors.initials: X
authors.lastname: Štramová
authors.affiliation: Department of Biological and Biochemical Sciences, Faculty of Chemical Technology, University of Pardubice, Studentska 573, 532 10 Pardubice, Czech Republic.
authors.forename: Petra
authors.initials: P
authors.lastname: Drábková
authors.affiliation: Department of Biological and Biochemical Sciences, Faculty of Chemical Technology, University of Pardubice, Studentska 573, 532 10 Pardubice, Czech Republic.
authors.forename: Martina
authors.initials: M
authors.lastname: Brandtnerová
mesh_terms: D000293:Adolescent; D000328:Adult; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D059788:Dried Blood Spot Testing / Q000379:methods*; D005260:Female; D005978:Glutathione / Q000097:blood* / Q000737:chemistry; D006801:Humans; D057230:Limit of Detection; D016014:Linear Models; D008297:Male; D000432:Methanol; D008875:Middle Aged; D015203:Reproducibility of Results; D055815:Young Adult
chemical_list: D005978:Glutathione; D000432:Methanol