title: Experimental evaluation does not reveal a direct effect of microRNA from the callipyge locus on DLK1 expression.
abstract: BACKGROUND
  Polar overdominance at the ovine callipyge (CLPG) locus involves the post-transcriptional trans-inhibition of DLK1 in skeletal muscle of CLPG/CLPG sheep. The abundant maternally expressed microRNAs (miRNAs) mapping to the imprinted DLK1-GTL2 domain are prime candidate mediators of this trans-effect.
  
  
  RESULTS
  We have tested the affinity of 121 miRNAs processed from this locus for DLK1 by co-transfecting COS1 cells with a vector expressing the full-length ovine DLK1 with corresponding mimic miRNAs. None of the tested miRNAs was able to down regulate DLK1 to the extent observed in vivo.
  
  
  CONCLUSIONS
  This suggests that other factors, with or without these miRNAs, are involved in mediating the observed trans-effect.
authors.forename: Huijun
authors.initials: H
authors.lastname: Cheng
authors.forename: Xuewen
authors.initials: X
authors.lastname: Xu
authors.forename: Tracy
authors.initials: T
authors.lastname: Hadfield
authors.forename: Noelle
authors.initials: N
authors.lastname: Cockett
authors.forename: Carole
authors.initials: C
authors.lastname: Charlier
authors.forename: Michel
authors.initials: M
authors.lastname: Georges
authors.affiliation: Unit of Animal Genomics, GIGA-R & Faculty of Veterinary Medicine, University of Liège (B34), 1 Avenue de l'Hôpital, 4000 Liège, Belgium. htakeda@ulg.ac.be.
authors.forename: Haruko
authors.initials: H
authors.lastname: Takeda
mesh_terms: D000818:Animals; D019556:COS Cells; D002522:Chlorocebus aethiops; D005786:Gene Expression Regulation; D018392:Genomic Imprinting; D036341:Intercellular Signaling Peptides and Proteins / Q000378:metabolism*; D035683:MicroRNAs / Q000378:metabolism*; D018482:Muscle, Skeletal / Q000378:metabolism*; D012756:Sheep / Q000235:genetics*; D014162:Transfection
chemical_list: D036341:Intercellular Signaling Peptides and Proteins; D035683:MicroRNAs