title: Multiple roles of extracellular fibroblast growth factors in lung cancer cells.
abstract: Cancer cells are surrounded by the extracellular fluid, matrix, and stroma cells. Little is known about how extracellular components such as growth factor ligands affect the biology of lung cancer cells. The objective of this study was to determine whether extracellular fibroblast growth factors (FGFs) can affect the biology of lung cancer cells and to understand how extracellular FGFs affect the biology of lung cancer cells, including non-small cell lung cancer (NSCLC) and small cell lung cancer (SCLC) cells. Out of the 23 reported FGFs, we focused on FGF2, FGF9 and FGF10. We examined the effect of FGFs on proliferation, treatment sensitivity, and apoptosis of NSCLC (PC9) and SCLC (H69, H82 and H146) cells in vitro. To determine which FGF was the most clinically relevant, we also examined FGF2 and FGF9 concentrations in the serum of patients with lung cancer. We found that extracellular FGFs can affect proliferation, treatment sensitivity, and apoptosis of lung cancer cells in a cell-specific manner. Our results indicate that extracellular FGFs affect the biology of lung cancer cells through multiple functions.
authors.affiliation: Keio University School of Medicine, Tokyo 160-8582, Japan.
authors.forename: Takahiro
authors.initials: T
authors.lastname: Suzuki
authors.affiliation: Division of Pulmonary Medicine, Department of Medicine, Keio University School of Medicine, Tokyo 160-8582, Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Yasuda
authors.affiliation: Keio University School of Medicine, Tokyo 160-8582, Japan.
authors.forename: Koji
authors.initials: K
authors.lastname: Funaishi
authors.affiliation: Division of Pulmonary Medicine, Department of Medicine, Keio University School of Medicine, Tokyo 160-8582, Japan.
authors.forename: Daisuke
authors.initials: D
authors.lastname: Arai
authors.affiliation: Division of Pulmonary Medicine, Department of Medicine, Keio University School of Medicine, Tokyo 160-8582, Japan.
authors.forename: Kota
authors.initials: K
authors.lastname: Ishioka
authors.affiliation: Division of Pulmonary Medicine, Department of Medicine, Keio University School of Medicine, Tokyo 160-8582, Japan.
authors.forename: Keiko
authors.initials: K
authors.lastname: Ohgino
authors.affiliation: Division of Pulmonary Medicine, Department of Medicine, Keio University School of Medicine, Tokyo 160-8582, Japan.
authors.forename: Tetsuo
authors.initials: T
authors.lastname: Tani
authors.affiliation: Division of Pulmonary Medicine, Department of Medicine, Keio University School of Medicine, Tokyo 160-8582, Japan.
authors.forename: Junko
authors.initials: J
authors.lastname: Hamamoto
authors.affiliation: Division of Pulmonary Medicine, Department of Medicine, Keio University School of Medicine, Tokyo 160-8582, Japan.
authors.forename: Ayano
authors.initials: A
authors.lastname: Ohashi
authors.affiliation: Keio Cancer Center, Keio University Hospital, Shinjuku-ku, Tokyo 160-8582, Japan.
authors.forename: Katsuhiko
authors.initials: K
authors.lastname: Naoki
authors.affiliation: Division of Pulmonary Medicine, Department of Medicine, Keio University School of Medicine, Tokyo 160-8582, Japan.
authors.forename: Tomoko
authors.initials: T
authors.lastname: Betsuyaku
authors.affiliation: Division of Pulmonary Medicine, Department of Medicine, Keio University School of Medicine, Tokyo 160-8582, Japan.
authors.forename: Kenzo
authors.initials: K
authors.lastname: Soejima
mesh_terms: D002289:Carcinoma, Non-Small-Cell Lung / Q000097:blood / Q000473:pathology; D049109:Cell Proliferation / Q000187:drug effects; D005110:Extracellular Space; D016222:Fibroblast Growth Factor 2 / Q000097:blood / Q000494:pharmacology / Q000502:physiology; D051525:Fibroblast Growth Factor 9 / Q000097:blood / Q000494:pharmacology / Q000502:physiology; D005346:Fibroblast Growth Factors / Q000097:blood / Q000494:pharmacology / Q000502:physiology*; D006801:Humans; D008175:Lung Neoplasms / Q000097:blood / Q000473:pathology*; D020935:MAP Kinase Signaling System / Q000187:drug effects; D051058:Oncogene Protein v-akt / Q000378:metabolism; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism; D055752:Small Cell Lung Carcinoma / Q000097:blood / Q000473:pathology; D014407:Tumor Cells, Cultured
chemical_list: C496459:FGF9 protein, human; D051525:Fibroblast Growth Factor 9; D016222:Fibroblast Growth Factor 2; D005346:Fibroblast Growth Factors; D019869:Phosphatidylinositol 3-Kinases; D051058:Oncogene Protein v-akt