title: Proteomes of host cell membranes modified by intracellular activities of Salmonella enterica.
abstract: Intracellular pathogens need to establish a growth-stimulating host niche for survival and replication. A unique feature of the gastrointestinal pathogen Salmonella enterica serovar Typhimurium is the creation of extensive membrane networks within its host. An understanding of the origin and function of these membranes is crucial for the development of new treatment strategies. However, the characterization of this compartment is very challenging, and only fragmentary knowledge of its composition and biogenesis exists. Here, we describe a new proteome-based approach to enrich and characterize Salmonella-modified membranes. Using a Salmonella mutant strain that does not form this unique membrane network as a reference, we identified a high-confidence set of host proteins associated with Salmonella-modified membranes. This comprehensive analysis allowed us to reconstruct the interactions of Salmonella with host membranes. For example, we noted that Salmonella redirects endoplasmic reticulum (ER) membrane trafficking to its intracellular niche, a finding that has not been described for Salmonella previously. Our system-wide approach therefore has the potential to rapidly close gaps in our knowledge of the infection process of intracellular pathogens and demonstrates a hitherto unrecognized complexity in the formation of Salmonella host niches.
authors.affiliation: From the ‡Division of Microbiology, School of Biology/Chemistry, University of Osnabrück, 49076 Osnabrück, Germany.
authors.forename: Stephanie
authors.initials: S
authors.lastname: Vorwerk
authors.affiliation: From the ‡Division of Microbiology, School of Biology/Chemistry, University of Osnabrück, 49076 Osnabrück, Germany.
authors.forename: Viktoria
authors.initials: V
authors.lastname: Krieger
authors.affiliation: From the ‡Division of Microbiology, School of Biology/Chemistry, University of Osnabrück, 49076 Osnabrück, Germany.
authors.forename: Jörg
authors.initials: J
authors.lastname: Deiwick
authors.affiliation: From the ‡Division of Microbiology, School of Biology/Chemistry, University of Osnabrück, 49076 Osnabrück, Germany Nicole.Hansmeier@biologie.uni-osnabrueck.de Michael.Hensel@biologie.uni-osnabrueck.de.
authors.forename: Michael
authors.initials: M
authors.lastname: Hensel
authors.affiliation: From the ‡Division of Microbiology, School of Biology/Chemistry, University of Osnabrück, 49076 Osnabrück, Germany Nicole.Hansmeier@biologie.uni-osnabrueck.de Michael.Hensel@biologie.uni-osnabrueck.de.
authors.forename: Nicole
authors.initials: N
authors.lastname: Hansmeier
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D002460:Cell Line; D004721:Endoplasmic Reticulum / Q000378:metabolism; D006367:HeLa Cells; D054884:Host-Pathogen Interactions; D006801:Humans; D008565:Membrane Proteins / Q000378:metabolism*; D051379:Mice; D020543:Proteome; D012480:Salmonella Infections / Q000378:metabolism; D019779:Salmonella enterica / Q000502:physiology*
chemical_list: D001426:Bacterial Proteins; D008565:Membrane Proteins; D020543:Proteome