title: Properties of neural crest-like cells differentiated from human embryonic stem cells.
abstract: Neural crest cells (NCCs) derive early in vertebrate ontogenesis from neural tube as a population of migratory cells with exquisite differentiation potential. Abnormalities in NCC behaviour are cause of debilitating diseases including cancers and a spectrum of neurocristopathies. Thanks to their multilineage differentiation capacity NCCs offer a cell source for regenerative medicine. Both these aspects make NCC biology an important issue to study, which can currently be addressed using methodologies based on pluripotent stem cells. Here we contributed to understanding the biology of human NCCs by refining the protocol for differentiation/propagation of NCClike cells from human embryonic stem cells and by characterizing the molecular and functional phenotype of such cells. Most importantly, we improved formulation of media for NCC culture, we found that poly-L-ornithine combined with fibronectin provide good support for NCC growth, we unravelled the tendency of cultured NCCs to maintain heterogeneity of CD271 expression, and we showed that NCCs derived here possess the capacity to react to BMP4 signals by dramatically up-regulating MSX1, which is linked to odontogenesis.
authors.affiliation: Department of Histology and Embryology, Faculty of Medicine, Masaryk University, Brno.
authors.forename: J
authors.initials: J
authors.lastname: Křivánek
authors.affiliation: Institute of Animal Physiology and Genetics, Academy of Sciences of the Czech Republic, Liběchov, Czech Republic.
authors.forename: E
authors.initials: E
authors.lastname: Švandová
authors.affiliation: Department of Histology and Embryology, Faculty of Medicine, Masaryk University, Brno.
authors.forename: J
authors.initials: J
authors.lastname: Králik
authors.affiliation: Department of Histology and Embryology, Faculty of Medicine, Masaryk University, Brno.
authors.forename: Š
authors.initials: Š
authors.lastname: Hajda
authors.affiliation: Department of Cytokinetics, Institute of Biophysics AS CR, v. v. i., Brno, Czech Republic.
authors.forename: R
authors.initials: R
authors.lastname: Fedr
authors.affiliation: Department of Histology and Embryology, Faculty of Medicine, Masaryk University, Brno.
authors.forename: V
authors.initials: V
authors.lastname: Vinarský
authors.affiliation: Department of Histology and Embryology, Faculty of Medicine, Masaryk University, Brno.
authors.forename: J
authors.initials: J
authors.lastname: Jaroš
authors.affiliation: International Clinical Research Centre, St. Anne's University Hospital, Brno, Czech Republic.
authors.forename: K
authors.initials: K
authors.lastname: Souček
authors.affiliation: Institute of Animal Physiology and Genetics, Academy of Sciences of the Czech Republic, Liběchov, Czech Republic.
authors.forename: M
authors.initials: M
authors.lastname: Buchtová
authors.affiliation: Institute of Animal Physiology and Genetics, Academy of Sciences of the Czech Republic, Liběchov, Czech Republic.
authors.forename: E
authors.initials: E
authors.lastname: Matalová
authors.affiliation: Department of Histology and Embryology, Faculty of Medicine, Masaryk University, Brno.
authors.forename: A
authors.initials: A
authors.lastname: Hampl
mesh_terms: D000068816:Adapalene; D015415:Biomarkers / Q000378:metabolism; D055415:Bone Morphogenetic Protein 4 / Q000494:pharmacology; D002454:Cell Differentiation / Q000187:drug effects; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D005434:Flow Cytometry; D006801:Humans; D051957:MSX1 Transcription Factor / Q000378:metabolism; D009281:Naphthalenes / Q000378:metabolism; D009432:Neural Crest / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D010641:Phenotype; D016133:Polymerase Chain Reaction
chemical_list: D015415:Biomarkers; D055415:Bone Morphogenetic Protein 4; D051957:MSX1 Transcription Factor; C484083:MSX1 protein, human; D009281:Naphthalenes; D000068816:Adapalene