title: Fluctuation of "sperm DNA integrity" in accordance with semen parameters, and it's relationship with infertility.
abstract: OBJECTIVE
  Nowadays sperm analysis is used routinely to assess infertile men, but it's predictive value is still restricted. For this reason, new markers are needed for diagnosis and guide treatment. A DNA damage evaluation method, which is easily applicable for routine analysis is described in this paper to provide an important support in clinical evaluation.
  
  
  METHODS
  In this study, DNA damage evaluation was planned in infertile males with the COMET assay, a method which is simple, cheap, sensitive and reliable. For this purpose, the sperm DNA damage of normospermic, oligospermic, asthenospermic and teratospermic cases were investigated and the relationship between sperm parameters (sperm count, motility, morphology) and DNA damage was assessed.
  
  
  CONCLUSIONS
  According to our results we suggest that the factors to blame for sperm DNA damage and semen quality are the same. Also it was found that the infertile males possess substantially more sperm DNA damage than fertile men do. Improving and using the DNA damage evaluation tests seem to improve the results in ART clinics.
authors.affiliation: Faculty of Medicine, Family Planning-Infertility Research and Treatment Center, Ege University, Bornova, Izmir, Turkey, durmazaysen79@gmail.com.
authors.forename: Ayşen
authors.initials: A
authors.lastname: Durmaz
authors.forename: Nurten
authors.initials: N
authors.lastname: Dikmen
authors.forename: Cumhur
authors.initials: C
authors.lastname: Gündüz
authors.forename: Ege Nazan Tavmergen
authors.initials: EN
authors.lastname: Göker
authors.forename: Erol
authors.initials: E
authors.lastname: Tavmergen
mesh_terms: D000328:Adult; D020552:Comet Assay / Q000379:methods; D004249:DNA Damage / Q000235:genetics; D005260:Female; D006801:Humans; D007248:Infertility, Male / Q000235:genetics* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D027724:Reproductive Techniques, Assisted; D055101:Semen Analysis; D013076:Sperm Count; D013081:Sperm Motility / Q000502:physiology; D013094:Spermatozoa / Q000473:pathology*