title: Gene expression of Hsps in normal and abnormal embryonic development of mouse hindlimbs.
abstract: Heat shock proteins (Hsps), which have important biological functions, are a class of highly conserved genetic molecules with the capacity of protecting and promoting cells to repair themselves from damage caused by various stimuli. Our previous studies found that Hsp25, HspB2, HspB3, HspB7, Hsp20, HspB9, HspB10, and Hsp40 may be related to all-trans retinoic acid (atRA)-induced phocomelic and other abnormalities, while HspA12B, HspA14, Trap1, and Hsp105 may be forelimb development-related genes; Grp78 may play an important role in forelimb development. In this study, the embryonic phocomelic, oligodactylic model of both forelimbs and hindlimbs was developed by atRA administered per os to the pregnant mice on gestational day 11, and the expression of 36 members of Hsps family in normal and abnormal development of embryonic hindlimbs was measured by real-time fluorescent quantitative polymerase chain reaction (qRT-PCR). It is found that HspA1L, Hsp22, Hsp10, Hsp60, Hsp47, HspB2, HspB10, HspA12A, Apg1, HspB4, Grp78, and HspB9 probably performs a major function in limb development, and HspA13, Grp94 and Hsp110 may be hindlimb development-related genes.
authors.affiliation: Medical School, Hunan Normal University, Changsha, People's Republic of China.
authors.forename: Zhengli
authors.initials: Z
authors.lastname: Yan
authors.affiliation: Department of Health Toxicology, Second Military Medical University, Shanghai, People's Republic of China.
authors.forename: Huimiao
authors.initials: H
authors.lastname: Wei
authors.affiliation: Department of Laboratory, No.100 Hospital of CPLA, Suzhou, People's Republic of China.
authors.forename: Chuanlu
authors.initials: C
authors.lastname: Ren
authors.affiliation: Medical School, Hunan Normal University, Changsha, People's Republic of China.
authors.forename: Shishan
authors.initials: S
authors.lastname: Yuan
authors.affiliation: Medical School, Hunan Normal University, Changsha, People's Republic of China.
authors.forename: Hu
authors.initials: H
authors.lastname: Fu
authors.affiliation: Medical School, Hunan Normal University, Changsha, People's Republic of China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Lv
authors.affiliation: Medical School, Hunan Normal University, Changsha, People's Republic of China njzhu70@126.com.
authors.forename: Yongfei
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: Department of Health Toxicology, Second Military Medical University, Shanghai, People's Republic of China tbzhang2009@yeah.net.
authors.forename: Tianbao
authors.initials: T
authors.lastname: Zhang
mesh_terms: D000818:Animals; D047108:Embryonic Development; D000091342:Endoplasmic Reticulum Chaperone BiP; D005260:Female; D015870:Gene Expression / Q000187:drug effects; D006360:Heat-Shock Proteins / Q000235:genetics*; D006614:Hindlimb / Q000196:embryology*; D008431:Maternal-Fetal Exchange; D008813:Mice, Inbred ICR; D011247:Pregnancy; D014212:Tretinoin / Q000633:toxicity*
chemical_list: D000091342:Endoplasmic Reticulum Chaperone BiP; D006360:Heat-Shock Proteins; C000717248:Hspa5 protein, mouse; D014212:Tretinoin
keywords: Heat shock proteins; all-trans retinoic acid; development; embryo; hindlimbs