title: MeDIP-on-Chip for methylation profiling.
abstract: DNA methylation is an important regulatory step in gene expression. Knowledge of the alterations in DNA methylation at a whole genome scale improves our understanding of gene regulation and potential correlations with biological events or disease progression. Methylated DNA immunoprecipitation (MeDIP) uses an antibody that efficiently enriches methylated DNA fragments for downstream locus-specific or genome-wide analyses. MeDIP-on-Chip uses the MeDIP approach in combination with a tiling array for the investigation of genome-wide DNA methylation patterns (or DNA methylomics). The following protocol describes the application of MeDIP to the hybridization of DNA microarrays and data analysis.
authors.affiliation: Department of Obstetrics and Gynecology, Tri-Service General Hospital and Graduate Institute of Life Sciences, National Defense Medical Center, Taipei City, Taiwan, ROC.
authors.forename: Yaw-Wen
authors.initials: YW
authors.lastname: Hsu
authors.forename: Rui-Lan
authors.initials: RL
authors.lastname: Huang
authors.forename: Hung-Cheng
authors.initials: HC
authors.lastname: Lai
mesh_terms: D018899:CpG Islands / Q000235:genetics; D004247:DNA / Q000378:metabolism; D019175:DNA Methylation / Q000235:genetics*; D015342:DNA Probes / Q000378:metabolism; D004587:Electrophoresis, Agar Gel; D015723:Gene Library; D006801:Humans; D047468:Immunoprecipitation / Q000379:methods*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D011401:Promoter Regions, Genetic / Q000235:genetics; D012685:Sepharose; D013223:Statistics as Topic
chemical_list: D015342:DNA Probes; D004247:DNA; D012685:Sepharose