title: The generation of kidney organoids by differentiation of human pluripotent cells to ureteric bud progenitor-like cells.
abstract: This protocol presents recently developed methodologies for the differentiation of human pluripotent stem cells (hPSCs) into ureteric bud (UB) progenitor-like cells. Differentiation of human PSCs to UB progenitor-like cells allows for the generation of chimeric kidney cultures in which the human cells can self-assemble into chimeric 3D structures in combination with embryonic mouse kidney cells over a period of 18 d. UB progenitor-like cells are generated by a two-step process that combines in vitro commitment of human PSCs, whether embryonic stem cells (ESCs) or induced PSCs (iPSCs), under chemically defined culture conditions, with ex vivo cultures for the induction of 3D organogenesis. The models described here provide new opportunities for investigating human kidney development, modeling disease, evaluating regenerative medicine strategies, as well as for toxicology studies.
authors.affiliation: Gene Expression Laboratory, Salk Institute for Biological Studies, La Jolla, California, USA.
authors.forename: Yun
authors.initials: Y
authors.lastname: Xia
authors.affiliation: Gene Expression Laboratory, Salk Institute for Biological Studies, La Jolla, California, USA.
authors.forename: Ignacio
authors.identifier: 0000000343481038
authors.initials: I
authors.lastname: Sancho-Martinez
authors.affiliation: Gene Expression Laboratory, Salk Institute for Biological Studies, La Jolla, California, USA.
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Nivet
authors.affiliation: Gene Expression Laboratory, Salk Institute for Biological Studies, La Jolla, California, USA.
authors.forename: Concepcion
authors.initials: C
authors.lastname: Rodriguez Esteban
authors.affiliation: Renal Division, Hospital Clinic, University of Barcelona, Institut d'investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), Barcelona, Spain.
authors.forename: Josep Maria
authors.initials: JM
authors.lastname: Campistol
authors.affiliation: Gene Expression Laboratory, Salk Institute for Biological Studies, La Jolla, California, USA.
authors.forename: Juan Carlos
authors.initials: JC
authors.lastname: Izpisua Belmonte
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D005260:Female; D006801:Humans; D007668:Kidney / Q000166:cytology* / Q000196:embryology; D008297:Male; D008815:Mice, Inbred Strains; D009924:Organ Culture Techniques / Q000379:methods*; D038081:Organogenesis; D009940:Organoids / Q000166:cytology*; D039904:Pluripotent Stem Cells / Q000166:cytology*