title: Potential effects of aerobic exercise on the expression of perilipin 3 in the adipose tissue of women with polycystic ovary syndrome: a pilot study.
abstract: OBJECTIVE
  Polycystic ovary syndrome (PCOS) is associated with reduced adipose tissue lipolysis that can be rescued by aerobic exercise. We aimed to identify differences in the gene expression of perilipins and associated targets in adipose tissue in women with PCOS before and after exercise.
  
  
  METHODS
  We conducted a cross-sectional study in eight women with PCOS and eight women matched for BMI and age with normal cycles. Women with PCOS also completed a 16-week prospective aerobic exercise-training study. Abdominal subcutaneous adipose tissue biopsies were collected, and primary adipose-derived stromal/stem cell cultures were established from women with PCOS before 16 weeks of aerobic exercise training (n=5) and controls (n=5). Gene expression was measured using real-time PCR, in vitro lipolysis was measured using radiolabeled oleate, and perilipin 3 (PLIN3) protein content was measured by western blotting analysis.
  
  
  RESULTS
  The expression of PLIN1, PLIN3, and PLIN5, along with coatomers ARF1, ARFRP1, and βCOP was ∼ 80% lower in women with PCOS (all P<0.05). Following exercise training, PLIN3 was the only perilipin to increase significantly (P<0.05), along with coatomers ARF1, ARFRP1, βCOP, and SEC23A (all P<0.05). Furthermore, PLIN3 protein expression was undetectable in the cell cultures from women with PCOS vs controls. Following exercise training, in vitro adipose oleate oxidation, glycerol secretion, and PLIN3 protein expression were increased, along with reductions in triglyceride content and absence of large lipid droplet morphology.
  
  
  CONCLUSIONS
  These findings suggest that PLIN3 and coatomer GTPases are important regulators of lipolysis and triglyceride storage in the adipose tissue of women with PCOS.
authors.affiliation: Laboratory of Reproductive Endocrinology and Woman's HealthPennington Biomedical Research Center, 6400 Perkins Road, Baton Rouge, Louisiana 70808, USADepartment of KinesiologyUniversity of Texas in El Paso, 500 University Avenue, El Paso, Texas 79968, USAInserm UMR 1048Institute of Metabolic and Cardiovascular Diseases and Paul Sabatier University, Toulouse, France.
authors.forename: Jeffrey D
authors.initials: JD
authors.lastname: Covington
authors.affiliation: Laboratory of Reproductive Endocrinology and Woman's HealthPennington Biomedical Research Center, 6400 Perkins Road, Baton Rouge, Louisiana 70808, USADepartment of KinesiologyUniversity of Texas in El Paso, 500 University Avenue, El Paso, Texas 79968, USAInserm UMR 1048Institute of Metabolic and Cardiovascular Diseases and Paul Sabatier University, Toulouse, France.
authors.forename: Sudip
authors.initials: S
authors.lastname: Bajpeyi
authors.affiliation: Laboratory of Reproductive Endocrinology and Woman's HealthPennington Biomedical Research Center, 6400 Perkins Road, Baton Rouge, Louisiana 70808, USADepartment of KinesiologyUniversity of Texas in El Paso, 500 University Avenue, El Paso, Texas 79968, USAInserm UMR 1048Institute of Metabolic and Cardiovascular Diseases and Paul Sabatier University, Toulouse, France.
authors.forename: Cedric
authors.initials: C
authors.lastname: Moro
authors.affiliation: Laboratory of Reproductive Endocrinology and Woman's HealthPennington Biomedical Research Center, 6400 Perkins Road, Baton Rouge, Louisiana 70808, USADepartment of KinesiologyUniversity of Texas in El Paso, 500 University Avenue, El Paso, Texas 79968, USAInserm UMR 1048Institute of Metabolic and Cardiovascular Diseases and Paul Sabatier University, Toulouse, France.
authors.forename: Yourka D
authors.initials: YD
authors.lastname: Tchoukalova
authors.affiliation: Laboratory of Reproductive Endocrinology and Woman's HealthPennington Biomedical Research Center, 6400 Perkins Road, Baton Rouge, Louisiana 70808, USADepartment of KinesiologyUniversity of Texas in El Paso, 500 University Avenue, El Paso, Texas 79968, USAInserm UMR 1048Institute of Metabolic and Cardiovascular Diseases and Paul Sabatier University, Toulouse, France.
authors.forename: Philip J
authors.initials: PJ
authors.lastname: Ebenezer
authors.affiliation: Laboratory of Reproductive Endocrinology and Woman's HealthPennington Biomedical Research Center, 6400 Perkins Road, Baton Rouge, Louisiana 70808, USADepartment of KinesiologyUniversity of Texas in El Paso, 500 University Avenue, El Paso, Texas 79968, USAInserm UMR 1048Institute of Metabolic and Cardiovascular Diseases and Paul Sabatier University, Toulouse, France.
authors.forename: David H
authors.initials: DH
authors.lastname: Burk
authors.affiliation: Laboratory of Reproductive Endocrinology and Woman's HealthPennington Biomedical Research Center, 6400 Perkins Road, Baton Rouge, Louisiana 70808, USADepartment of KinesiologyUniversity of Texas in El Paso, 500 University Avenue, El Paso, Texas 79968, USAInserm UMR 1048Institute of Metabolic and Cardiovascular Diseases and Paul Sabatier University, Toulouse, France.
authors.forename: Eric
authors.initials: E
authors.lastname: Ravussin
authors.affiliation: Laboratory of Reproductive Endocrinology and Woman's HealthPennington Biomedical Research Center, 6400 Perkins Road, Baton Rouge, Louisiana 70808, USADepartment of KinesiologyUniversity of Texas in El Paso, 500 University Avenue, El Paso, Texas 79968, USAInserm UMR 1048Institute of Metabolic and Cardiovascular Diseases and Paul Sabatier University, Toulouse, France leanne.redman@pbrc.edu.
authors.forename: Leanne M
authors.initials: LM
authors.lastname: Redman
mesh_terms: D000273:Adipose Tissue / Q000378:metabolism*; D000328:Adult; D003430:Cross-Sectional Studies; D015444:Exercise / Q000502:physiology*; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D000070796:Perilipin-3; D010865:Pilot Projects; D011085:Polycystic Ovary Syndrome / Q000378:metabolism* / Q000628:therapy*; D011446:Prospective Studies; D016896:Treatment Outcome; D033921:Vesicular Transport Proteins / Q000096:biosynthesis*; D055815:Young Adult
chemical_list: C038196:PLIN3 protein, human; D000070796:Perilipin-3; D033921:Vesicular Transport Proteins