title: Master regulators, regulatory networks, and pathways of glioblastoma subtypes.
abstract: Glioblastoma multiforme (GBM) is the most common malignant brain tumor. GBM samples are classified into subtypes based on their transcriptomic and epigenetic profiles. Despite numerous studies to better characterize GBM biology, a comprehensive study to identify GBM subtype- specific master regulators, gene regulatory networks, and pathways is missing. Here, we used FastMEDUSA to compute master regulators and gene regulatory networks for each GBM subtype. We also ran Gene Set Enrichment Analysis and Ingenuity Pathway Analysis on GBM expression dataset from The Cancer Genome Atlas Project to compute GBM- and GBM subtype-specific pathways. Our analysis was able to recover some of the known master regulators and pathways in GBM as well as some putative novel regulators and pathways, which will aide in our understanding of the unique biology of GBM subtypes.
authors.affiliation: Neuro-Oncology Branch, National Cancer Institute, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, Maryland, USA. ; Department of Mathematics, Statistics, and Computer Science, Marquette University, Milwaukee, Wisconsin, USA.
authors.forename: Serdar
authors.initials: S
authors.lastname: Bozdag
authors.affiliation: Neuro-Oncology Branch, National Cancer Institute, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, Maryland, USA.
authors.forename: Aiguo
authors.initials: A
authors.lastname: Li
authors.affiliation: Neuro-Oncology Branch, National Cancer Institute, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, Maryland, USA. ; New York University Cancer Institute, New York University Langone Medical Center, New York, New York, USA.
authors.forename: Mehmet
authors.initials: M
authors.lastname: Baysan
authors.affiliation: Neuro-Oncology Branch, National Cancer Institute, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, Maryland, USA. ; New York University Cancer Institute, New York University Langone Medical Center, New York, New York, USA.
authors.forename: Howard A
authors.initials: HA
authors.lastname: Fine
keywords: GBM; GBM subtypes; classical; gene regulatory networks; glioblastoma multiforme; master regulators; mesenchymal; neural; pathways; proneural