title: Development of amperometric glucose biosensor based on Prussian Blue functionlized TiO2 nanotube arrays.
abstract: Amperometric biosensors consisting of oxidase and peroxidase have attracted great attention because of their wide application. The current work demonstrates a novel approach to construct an enzymatic biosensor based on TiO2 nanotube arrays (TiNTs) as a supporting electrode on which Prussian Blue (PB)-an "artificial enzyme peroxidase" and enzyme glucose oxidase (GOx) have been immobilized. For this, PB nanocrystals are deposited onto the nanotube wall photocatalytically using the intrinsic photocatalytical property of TiO2, and the GOx/AuNPs nanobiocomposites are subsequently immobilized into the nanotubes via the electrodeposition of polymer. The resulting electrode exhibits a fast response, wide linear range, and good stability for glucose sensing. The sensitivity of the sensor is as high as 248 mA M(-1) cm(-2), and the detection limit is about 3.2 μM. These findings demonstrate a promising strategy to integrate enzymes and TiNTs, which could provide an analytical access to a large group of enzymes for bioelectrochemical applications including biosensors and biofuel cells.
authors.affiliation: 1] College of Sciences, Northeastern University, Shenyang 110004, China [2] National Laboratory of Solid State Microstructures, Nanjing University, Nanjing 210093, China.
authors.forename: Zhi-Da
authors.initials: ZD
authors.lastname: Gao
authors.affiliation: College of Sciences, Northeastern University, Shenyang 110004, China.
authors.forename: Yongfang
authors.initials: Y
authors.lastname: Qu
authors.affiliation: College of Sciences, Northeastern University, Shenyang 110004, China.
authors.forename: Tongtong
authors.initials: T
authors.lastname: Li
authors.affiliation: Department of Chemistry, Hanyang University, Haengdang-dong 17, Sungdong-ku, Seoul 133-791, South Korea.
authors.forename: Nabeen K
authors.initials: NK
authors.lastname: Shrestha
authors.affiliation: College of Sciences, Northeastern University, Shenyang 110004, China.
authors.forename: Yan-Yan
authors.initials: YY
authors.lastname: Song
mesh_terms: D001674:Bioelectric Energy Sources; D015374:Biosensing Techniques; D001786:Blood Glucose; D004566:Electrodes; D004800:Enzymes, Immobilized / Q000737:chemistry; D005295:Ferrocyanides / Q000737:chemistry*; D005947:Glucose / Q000032:analysis*; D005949:Glucose Oxidase / Q000737:chemistry; D006801:Humans; D057230:Limit of Detection; D043942:Nanotubes / Q000737:chemistry*; D009195:Peroxidase / Q000737:chemistry; D014025:Titanium / Q000737:chemistry*
chemical_list: D001786:Blood Glucose; D004800:Enzymes, Immobilized; D005295:Ferrocyanides; C009495:titanium dioxide; D014025:Titanium; D005949:Glucose Oxidase; D009195:Peroxidase; D005947:Glucose; C000170:ferric ferrocyanide