title: An upconversion nanocomposite for fluorescence resonance energy transfer based cholesterol-sensing in human serum.
abstract: Upconversion nanophosphors (UCNPs) are extremely useful for analytical applications, since they display a high signal-to-noise ratio, and their photobleaching can be ignored. Herein, a novel upconversion nanocomposite composed of β-cyclodextrin (β-CD) derivative modified UCNPs and rhodamine B (RB) was prepared for the detection of cholesterol (Cho). The upconversion luminescence (UCL) emission can serve as a Cho-sensing signal by an effective fluorescence resonance energy transfer (FRET) process, using UCNPs as the donor and RB as the quencher. The sensor for Cho detection in human serum shows excellent sensitivity and selectivity, which has the potential for clinical applications in the analysis of other biological and environmental samples.
authors.affiliation: State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, 210093, China. jjzhu@nju.edu.cn jianglp@nju.edu.cn.
authors.forename: Yujie
authors.initials: Y
authors.lastname: Ding
authors.forename: Hao
authors.initials: H
authors.lastname: Zhu
authors.forename: Xiaoxia
authors.initials: X
authors.lastname: Zhang
authors.forename: Jiangang
authors.initials: J
authors.lastname: Gao
authors.forename: E S
authors.initials: ES
authors.lastname: Abdel-Halim
authors.forename: Liping
authors.initials: L
authors.lastname: Jiang
authors.forename: Jun-Jie
authors.initials: JJ
authors.lastname: Zhu
mesh_terms: D000328:Adult; D002784:Cholesterol / Q000097:blood*; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D006801:Humans; D053761:Nanocomposites / Q000032:analysis* / Q000737:chemistry*; D015203:Reproducibility of Results; D012235:Rhodamines / Q000097:blood* / Q000737:chemistry; D012680:Sensitivity and Specificity; D047392:beta-Cyclodextrins / Q000097:blood* / Q000737:chemistry
chemical_list: D012235:Rhodamines; D047392:beta-Cyclodextrins; D002784:Cholesterol; C029773:rhodamine B