title: Development of multiplex PCR assay for simultaneous detection of Salmonella genus, Salmonella subspecies I, Salm. Enteritidis, Salm. Heidelberg and Salm. Typhimurium.
abstract: OBJECTIVE
  The aim of this research was to develop multiplex PCR assay that could simultaneously detect Salmonella genus, Salmonella subsp. I, Salm. Enteritidis, Heidelberg and Typhimurium because these Salmonella serovars are the most common isolates associated with poultry products.
  
  
  RESULTS
  Five primers were utilized to establish multiplex PCR and applied to Salmonella isolates from chickens and farm environments. These isolates were identified as Salmonella subsp. I and 16 of 66 isolates were classified as Salm. Enteritidis, while Heidelberg or Typhimurium was not detected. We also spiked three Salmonella strains on chicken breast meat to evaluate the specificity and sensitivity of multiplex PCR as well as qPCR to optimize quantification of Salmonella in these samples. The optimized multiplex PCR and qPCR could detect approx. 2·2 CFU of Salmonella per gram after 18 h enrichment.
  
  
  CONCLUSIONS
  The multiplex PCR and qPCR would provide rapid and consistent results. Also, these techniques would be useful for the detection and quantification of Salmonella in contaminated poultry, foods and environmental samples.
  
  
  CONCLUSIONS
  The strategy for the rapid detection of Salmonella serovars in poultry is needed to further reduce the incidence of salmonellosis in humans. The optimized multiplex PCR will be useful to detect prevalent Salmonella serovars in poultry products.
authors.affiliation: Cell and Molecular Biology Program, Department of Food Science, University of Arkansas, Fayetteville, AR, USA; Center for Food Safety, Department of Food Science, University of Arkansas, Fayetteville, AR, USA.
authors.forename: S H
authors.initials: SH
authors.lastname: Park
authors.forename: S C
authors.initials: SC
authors.lastname: Ricke
mesh_terms: D000818:Animals; D002645:Chickens / Q000382:microbiology; D017931:DNA Primers; D008460:Meat / Q000382:microbiology; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods*; D060888:Real-Time Polymerase Chain Reaction; D012475:Salmonella / Q000235:genetics / Q000302:isolation & purification*; D019779:Salmonella enterica / Q000235:genetics / Q000302:isolation & purification*; D012477:Salmonella enteritidis / Q000235:genetics / Q000302:isolation & purification*; D012486:Salmonella typhimurium / Q000235:genetics / Q000302:isolation & purification*; D065288:Serogroup
chemical_list: D017931:DNA Primers
keywords: Salmonella; chicken breast meat; enrichment; multiplex PCR; quantitative PCR