title: Effect of Withania somnifera root extract on spontaneous estrogen receptor-negative mammary cancer in MMTV/Neu mice.
abstract: The cancer-preventive activity of an extract of Withania somnifera (WS) roots was examined in female transgenic (MMTV/Neu) mice that received a diet containing the extract (750 mg/kg of diet) for 10 months. Mice in the treated group (n=35) had an average of 1.66 mammary carcinomas, and mice in the control group (n=33) had 2.48, showing a reduction of 33%. The average weights of the carcinomas were 2.36 g for mice in the treated group and 2.63 g for the controls, a difference of 10%. Labeling indices for Ki67 and proliferating cell nuclear antigen marker in mammary carcinomas of the treated group were 35% and 30% lower, respectively, than those of the corresponding control group. Expression of the chemokine was reduced by 50%. These results indicate that the root extract reduced the number of mammary carcinomas that developed and reduced the rate of cell division in the carcinomas.
authors.affiliation: Department of Biomedical Sciences School of Veterinary Medicine, Tuskegee University, Tuskegee, AL, U.S.A.
authors.forename: Kamel F
authors.initials: KF
authors.lastname: Khazal
authors.affiliation: Division of Preventive Medicine, University of Alabama at Birmingham, Birmingham, AL, U.S.A.
authors.forename: Donald L
authors.initials: DL
authors.lastname: Hill
authors.affiliation: Department of Surgery, University of Alabama at Birmingham, Birmingham, AL, U.S.A. clintongrubbs@uabmc.edu.
authors.forename: Clinton J
authors.initials: CJ
authors.lastname: Grubbs
mesh_terms: D000818:Animals; D014408:Biomarkers, Tumor / Q000378:metabolism; D005260:Female; D007124:Immunoenzyme Techniques; D008325:Mammary Neoplasms, Experimental / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D008324:Mammary Tumor Virus, Mouse / Q000235:genetics; D051379:Mice; D008822:Mice, Transgenic; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology*; D018517:Plant Roots / Q000737:chemistry*; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000235:genetics*; D011960:Receptors, Estrogen / Q000378:metabolism*; D014407:Tumor Cells, Cultured; D032341:Withania / Q000737:chemistry*
chemical_list: D014408:Biomarkers, Tumor; D010936:Plant Extracts; D018719:Erb-b2 Receptor Tyrosine Kinases; D011960:Receptors, Estrogen; C524056:Erbb2 protein, mouse
keywords: Withania; chemoprevention; mammary cancer; mice