title: ROS-mediated cytotoxic effect of copper(II) hydrazone complexes against human glioma cells.
abstract: 2-Acetylpyridine acetylhydrazone (H2AcMe), 2-benzoylpyridine acetylhydrazone (H2BzMe) and complexes [Cu(H2AcMe)Cl2] (1) and [Cu(H2BzMe)Cl2] (2) were assayed for their cytotoxicity against wild type p53 U87 and mutant p53 T98 glioma cells, and against MRC-5 fibroblast cells. Compounds 1 and 2 proved to be more active than the corresponding hydrazones against U87, but not against T98 cells. Compound 1 induced higher levels of ROS than H2AcMe in both glioma cell lines. H2AcMe and 1 induced lower levels of ROS in MRC5 than in U87 cells. Compound 2 induced lower levels of ROS in MRC5 than in T98 cells. The cytotoxic effect of 1 in U87 cells could be related to its ability to provoke the release of ROS, suggesting that the cytotoxicity of 1 might be somehow p53 dependent.
authors.affiliation: Departamento de Química, Universidade Federal de Minas Gerais, 31270-901 Belo Horizonte, Brazil. areciod@yahoo.com.
authors.forename: Angel A
authors.initials: AA
authors.lastname: Recio Despaigne
authors.affiliation: Departamento de Química, Universidade Federal de Minas Gerais, 31270-901 Belo Horizonte, Brazil. jefersonpi1@yahoo.com.br.
authors.forename: Jeferson G
authors.initials: JG
authors.lastname: Da Silva
authors.affiliation: Centro de Desenvolvimento da Tecnologia Nuclear (CDTN), 31270-901 Belo Horizonte, Brazil. pryrodriigues@yahoo.com.br.
authors.forename: Pryscila R
authors.initials: PR
authors.lastname: da Costa
authors.affiliation: Centro de Desenvolvimento da Tecnologia Nuclear (CDTN), 31270-901 Belo Horizonte, Brazil. raqueou@uol.com.br.
authors.forename: Raquel G
authors.initials: RG
authors.lastname: Dos Santos
authors.affiliation: Departamento de Química, Universidade Federal de Minas Gerais, 31270-901 Belo Horizonte, Brazil. hberaldo@ufmg.br.
authors.forename: Heloisa
authors.initials: H
authors.lastname: Beraldo
mesh_terms: D002460:Cell Line; D045744:Cell Line, Tumor; D003300:Copper / Q000494:pharmacology*; D005347:Fibroblasts / Q000187:drug effects; D005910:Glioma / Q000188:drug therapy* / Q000378:metabolism; D006801:Humans; D006835:Hydrazones / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism*; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism
chemical_list: D006835:Hydrazones; D017382:Reactive Oxygen Species; D016159:Tumor Suppressor Protein p53; D003300:Copper