title: Comparative studies on the interaction of cefixime with bovine serum albumin by fluorescence quenching spectroscopy and synchronous fluorescence spectroscopy.
abstract: Under simulated physiological conditions, the reaction mechanism between cefixime and bovine serum albumin at different temperatures (293, 303 and 310 K) was investigated using a fluorescence quenching method and synchronous fluorescence method, respectively. The results indicated that the fluorescence intensity and synchronous fluorescence intensity of bovine serum albumin decreased regularly on the addition of cefixime. In addition, the quenching mechanism, binding constants, number of binding sites, type of interaction force and energy-transfer parameters of cefixime with bovine serum albumin obtained from two methods using the same equation were consistent. The results indicated that the synchronous fluorescence spectrometry could be used to study the binding mechanism between drug and protein, and was a useful supplement to the conventional method.
authors.affiliation: Key Laboratory of Medical Chemistry and Molecular Diagnosis, Hebei University, Baoding, People's Republic of China.
authors.forename: Lihui
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Medical Chemistry and Molecular Diagnosis, Hebei University, Baoding, People's Republic of China.
authors.forename: Baosheng
authors.initials: B
authors.lastname: Liu
authors.affiliation: Key Laboratory of Medical Chemistry and Molecular Diagnosis, Hebei University, Baoding, People's Republic of China.
authors.forename: Zhiyun
authors.initials: Z
authors.lastname: Li
authors.affiliation: Key Laboratory of Medical Chemistry and Molecular Diagnosis, Hebei University, Baoding, People's Republic of China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Guo
mesh_terms: D001665:Binding Sites; D020682:Cefixime / Q000737:chemistry* / Q000378:metabolism*; D012710:Serum Albumin, Bovine / Q000737:chemistry* / Q000378:metabolism*; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013056:Spectrophotometry, Ultraviolet
chemical_list: D012710:Serum Albumin, Bovine; D020682:Cefixime
keywords: bovine serum albumin; cefixime; fluorescence quenching spectroscopy; interaction; synchronous fluorescence