title: Lateral resolution in NALDI MSI: back to the future.
abstract: Nanostructure-assisted laser desorption/ionization (NALDI) has been recognized as a powerful matrix-free mass spectrometry tool ideal for imaging of small molecules. In this report, the NALDI approach was compared with matrix-assisted laser desorption/ionization (MALDI) mass spectrometry in terms of sensitivity, reproducibility, and lateral resolution, which can be achieved in mass spectrometry imaging (MSI) experiments using a Nd:YAG laser. Scanning electron microscopy was used for surface topology analysis and evaluation of a putative surface-enhanced sensitivity effect, which was observed upon reduction of the laser focus. NALDI was identified as a more reproducible technique lacking MSI artifacts arising from distant tissue removal known from MALDI oversampling.
authors.affiliation: Institute of Microbiology, v.v.i., Videnska 1083, 14220, Prague 4, Czech Republic.
authors.forename: Lukas
authors.initials: L
authors.lastname: Krasny
authors.forename: Oldřich
authors.initials: O
authors.lastname: Benada
authors.forename: Marcela
authors.initials: M
authors.lastname: Strnadova
authors.forename: Karel
authors.initials: K
authors.lastname: Lemr
authors.forename: Vladimir
authors.initials: V
authors.lastname: Havlicek
mesh_terms: D000818:Animals; D003952:Diagnostic Imaging; D007668:Kidney / Q000737:chemistry*; D007834:Lasers; D051379:Mice; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000295:instrumentation / Q000379:methods; D053719:Tandem Mass Spectrometry / Q000295:instrumentation / Q000379:methods*