title: Evaluation of Thrombin Generation Assay in Patients With Hemophilia.
abstract: We evaluated the correlation between thrombin generation (TG) parameters with bleeding symptoms and disease severity in patients with hemophilia. In this cross-sectional study, 59 patients with hemophilia without inhibitors and regardless of their severity were randomly selected from southern Iran and TG assays were conducted. Bleeding score (BS) was calculated by performing a clinical evaluation using Tosetto questionnaire. Only lag time showed a statistically significant correlation with BS (rs = .316,P= .016). All TG parameters except peak showed association with disease severity (P< .05). Endogenous thrombin potential showed a significant correlation with factor activity level (rs = .459,P< .001). Both lag time and start tail showed significant negative correlations with factor activity level (rs = -0.488,P< .001 andrs = - .289,P< .026, respectively). Although most of the TG parameters evaluated were not significantly correlated with the BS of patients with hemophilia, the majority of TG parameters were significantly associated with factor activity level and disease severity.
authors.affiliation: Hematology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Sezaneh
authors.initials: S
authors.lastname: Haghpanah
authors.affiliation: Hematology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Asghar
authors.initials: A
authors.lastname: Bazrafshan
authors.affiliation: Hematology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Samir
authors.initials: S
authors.lastname: Silavizadeh
authors.affiliation: Hematology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Javad
authors.initials: J
authors.lastname: Dehghani
authors.affiliation: Hematology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Abdolreza
authors.initials: A
authors.lastname: Afrasiabi
authors.affiliation: Hematology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran karimim@sums.ac.ir.
authors.forename: Mehran
authors.initials: M
authors.lastname: Karimi
mesh_terms: D019774:Blood Coagulation Factor Inhibitors / Q000097:blood*; D005260:Female; D006467:Hemophilia A / Q000097:blood*; D006801:Humans; D007492:Iran; D008297:Male; D012720:Severity of Illness Index; D013917:Thrombin / Q000378:metabolism*; D013918:Thrombin Time / Q000379:methods
chemical_list: D019774:Blood Coagulation Factor Inhibitors; D013917:Thrombin
keywords: bleeding; blood coagulation tests; hemophilia; hemostasis; thrombin time