title: RNA-RNA SELEX.
abstract: Systematic evolution of ligands by exponential enrichment (SELEX) protocol is a valuable technique to identify RNA aptamers interacting with RNA structural motifs. RNA aptamers are mainly resolved with affinity column chromatography and electrophoretic mobility shift assay (EMSA). Here, we describe the separation of the RNA aptamers binding to an RNA stem-loop target with affinity chromatography using the column attached the target RNA and nondenaturing polyacrylamide gel electrophoresis to obtain a single predominant RNA aptamer family.
authors.affiliation: Department of Applied Chemistry, Cheongju University, 298 Daeseongro, Cheongju Chungbuk, 360-764, S. Korea, brcho@cju.ac.kr.
authors.forename: B
authors.initials: B
authors.lastname: Cho
mesh_terms: D002846:Chromatography, Affinity; D024202:Electrophoretic Mobility Shift Assay; D015723:Gene Library; D012313:RNA / Q000378:metabolism*; D052156:SELEX Aptamer Technique / Q000379:methods*
chemical_list: D012313:RNA