title: A rapid new approach for the quality evaluation of the folk medicine Dianbaizhu based on chemometrics.
abstract: Dianbaizhu, a folk medicine from Gaultheria leucocarpa BLUME var. yunnanensis (FRANCH.) T. Z. HSU & R. C. FANG (Ericaceae) used as an antirheumatic, has multiple plant origins and officinal parts. A rapid high-performance liquid chromatography with diode array detector (HPLC-DAD) method was established for the simultaneous determination of the characteristic ingredient methyl benzoate-2-O-β-D-glucopyranosyl(1 → 2) [O-β-D-xylopyranosyl(1 → 6)]-O-β-D-glucopyranoside and seven bioactive constituents in eight Gaultheria species. This chromatographic method is precise, accurate, and stable. Kruskal-Wallis analysis, hierarchical cluster analysis, and factor analysis were used to analyze the content of reference compounds in different Gaultheria species and officinal parts. The analyses showed significant differences (p<0.05) in Gaultheria species but few differences (p>0.05) in their medicinal parts. G. leucocarpa var. yunnanensis appeared to the best among the Gaultheria species tested for the treatment of rheumatic diseases. Taken together, the results show that this simultaneous quantiﬁcation of multiple active constituents using HPLC-DAD combined with chemometrics can be reliably applied to evaluate the quality of Dianbaizhu.
authors.affiliation: School of Chinese Pharmacy, Beijing University of Chinese Medicine.
authors.forename: Zizhen
authors.initials: Z
authors.lastname: Liu
authors.forename: Rui
authors.initials: R
authors.lastname: Jiang
authors.forename: Meng
authors.initials: M
authors.lastname: Xie
authors.forename: Guanling
authors.initials: G
authors.lastname: Xu
authors.forename: Weirui
authors.initials: W
authors.lastname: Liu
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Wang
authors.forename: Hongying
authors.initials: H
authors.lastname: Lin
authors.forename: Jianqiu
authors.initials: J
authors.lastname: Lu
authors.forename: Gaimei
authors.initials: G
authors.lastname: She
mesh_terms: D018501:Antirheumatic Agents / Q000032:analysis / Q000302:isolation & purification*; D001565:Benzoates / Q000032:analysis / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D016000:Cluster Analysis; D031282:Gaultheria / Q000737:chemistry*; D005960:Glucosides / Q000032:analysis / Q000302:isolation & purification*; D008519:Medicine, Traditional; D010936:Plant Extracts / Q000737:chemistry*
chemical_list: D018501:Antirheumatic Agents; D001565:Benzoates; D005960:Glucosides; D010936:Plant Extracts; C044605:methyl benzoate