title: The potential signaling pathway between peroxisome proliferator-activated receptor gamma and retinoic acid receptor alpha in renal interstitial fibrosis disease.
abstract: Peroxisome proliferator-activated receptorγ (PPARγ) can regulate the process of cell apoptosis and is related to the progression of renal disorders. Retinoic acid receptor alpha (RARα) is one of the nuclear receptors involved in a variety of kidney diseases. Renal interstitial fibrosis (RIF) is a common denominator of chronic kidney disease (CKD). This study investigated whether a potential signaling pathway existed between PPARγ and RARα in RIF rats with unilateral ureteral obstruction (UUO). The rats were randomly divided into four groups: a model group subjected to UUO (GU), and three other groups treated with rosiglitazone sodium (GRS), GW9662 and dimethyl sulfoxide (DMSO), n = 40, respectively. Renal tissues were collected two and four weeks after post-surgery. The relevant indicators were detected. In comparison with the GU group, the expressions of PPARγ and RARα (protein and mRNA) were increased in the GRS group, and decreased in the GW9662 group (all p < 0.01). The RIF index, mRNA and protein expression of transforming growth factor-β1 (TGF-β1), and the protein expressions of collagen-IV (Col-IV) and fibronectin (FN) in the GRS group were more markedly reduced than those in the GU group; their levels in the GW9662 group were elevated (all p < 0.01). PPARγ or RARα was negatively correlated to the RIF index, TGF-β1, Col-IV and FN. PPARγ was positively correlated with RARα (all p < 0.01). In conclusion, PPARγ agonist can elevate the expression of PPARγ or RARα in RIF rats. There might be a potential signaling pathway between PPARγ and RARα in RIF disease.
authors.affiliation: a Department of Pediatrics , The First Affiliated Hospital of GuangXi Medical University , Nanning , China and.
authors.forename: Ling
authors.initials: L
authors.lastname: Jiang
authors.affiliation: a Department of Pediatrics , The First Affiliated Hospital of GuangXi Medical University , Nanning , China and.
authors.forename: Xiu-Ping
authors.initials: XP
authors.lastname: Chen
authors.affiliation: a Department of Pediatrics , The First Affiliated Hospital of GuangXi Medical University , Nanning , China and.
authors.forename: Yao-Bin
authors.initials: YB
authors.lastname: Long
authors.affiliation: a Department of Pediatrics , The First Affiliated Hospital of GuangXi Medical University , Nanning , China and.
authors.forename: Feng-Ying
authors.initials: FY
authors.lastname: Lei
authors.affiliation: a Department of Pediatrics , The First Affiliated Hospital of GuangXi Medical University , Nanning , China and.
authors.forename: Zhi-Qiang
authors.initials: ZQ
authors.lastname: Zhou
authors.affiliation: a Department of Pediatrics , The First Affiliated Hospital of GuangXi Medical University , Nanning , China and.
authors.forename: Yuan-Han
authors.initials: YH
authors.lastname: Qin
authors.affiliation: a Department of Pediatrics , The First Affiliated Hospital of GuangXi Medical University , Nanning , China and.
authors.forename: Wei-Fang
authors.initials: WF
authors.lastname: Huang
authors.affiliation: b Department of Nephrology , The Six Affiliated Hospital of Sun Yat-Sen University , Guangzhou , China.
authors.forename: Tian-Biao
authors.initials: TB
authors.lastname: Zhou
mesh_terms: D000818:Animals; D024141:Collagen Type IV / Q000378:metabolism; D005353:Fibronectins / Q000378:metabolism; D005355:Fibrosis; D015870:Gene Expression; D007150:Immunohistochemistry; D007668:Kidney / Q000378:metabolism / Q000473:pathology; D008297:Male; D047495:PPAR gamma / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D018168:Receptors, Retinoic Acid / Q000235:genetics / Q000378:metabolism*; D051436:Renal Insufficiency, Chronic / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*; D000072482:Retinoic Acid Receptor alpha; D015398:Signal Transduction; D053773:Transforming Growth Factor beta1 / Q000235:genetics / Q000378:metabolism
chemical_list: D024141:Collagen Type IV; D005353:Fibronectins; D047495:PPAR gamma; D012333:RNA, Messenger; C000607073:Rara protein, rat; D018168:Receptors, Retinoic Acid; D000072482:Retinoic Acid Receptor alpha; D053773:Transforming Growth Factor beta1
keywords: Peroxisome proliferator-activated receptorγ; renal interstitial fibrosis; retinoic acid receptor alpha; signaling pathway