title: Y-chromosomal haplotyping of single sperm cells isolated from semen mixtures - a successful identification of three perpetrators in a multi-suspect sexual assault case.
abstract: To obtain individual Y-short tandem repeat (STR) profiles in a multi-suspect sexual assault case. Methods. We used laser cut microdissection to capture the single sperm cell in the multi-contributor semen sample, combined with the low volume polymerase chain reaction (LV-PCR) method to genotype the single sperm cell profiles using the Yfiler(®) kit. Consensus DNA profiles were generated from 5 replicate experiments. Results. Ninety-four parallel LV-PCRs were performed and 41 reactions (44%) produced Y-STR profiles with more than nine loci. Three individual Y-STR profiles were successfully obtained. Conclusion. The three Y haplotype units matched three known perpetrators' genotypes. Our results showed that single sperm cells Y-STR analysis was a powerful method for analyzing multi-donor semen mixture sample.
authors.forename: Lei
authors.initials: L
authors.lastname: Feng
authors.forename: Cheng
authors.initials: C
authors.lastname: Xu
authors.forename: Xianhai
authors.initials: X
authors.lastname: Zeng
authors.forename: Huaicai
authors.initials: H
authors.lastname: Zhang
authors.forename: Fan
authors.initials: F
authors.lastname: Yang
authors.forename: Wanshui
authors.initials: W
authors.lastname: Li
authors.forename: Zheng
authors.initials: Z
authors.lastname: Tu
authors.forename: Caixia
authors.initials: C
authors.lastname: Li
authors.affiliation: Institute of Forensic Science, Ministry of Public Security, Beijing, 100038, China, 13911162826@139.com.
authors.forename: Lan
authors.initials: L
authors.lastname: Hu
mesh_terms: D041322:Chromosomes, Human, Y / Q000235:genetics*; D004247:DNA / Q000235:genetics; D016172:DNA Fingerprinting / Q000379:methods; D005260:Female; D005838:Genotype; D006239:Haplotypes; D006801:Humans; D008297:Male; D018895:Microsatellite Repeats / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods; D020641:Polymorphism, Single Nucleotide; D011902:Rape; D013094:Spermatozoa / Q000378:metabolism*; D014626:Vaginal Smears
chemical_list: D004247:DNA