title: Purification of Proteins From Cell-Culture Medium or Cell-Lysate by High-Speed Counter-Current Chromatography Using Cross-Axis Coil Planet Centrifuge.
abstract: This review describes protein purifications from cell culture medium or cell-lysate by high speed counter-current chromatography using the cross-axis coil planet centrifuge. Purifications were performed using aqueous two phase systems composed of polyethylene glycols and dextrans.
authors.affiliation: Division of Pharmaceutical and Biomedical Analysis, School of Pharmacy, Tokyo University of Pharmacy and Life Science, 1432-1 Horinouchi Hachioji, Tokyo 192-0392, Japan.
authors.forename: Yoichi
authors.initials: Y
authors.lastname: Shibusawa
authors.affiliation: Center for Biochemistry and Biophysics, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892-8014, USA.
authors.forename: Yoichiro
authors.initials: Y
authors.lastname: Ito