title: Structure and microbial diversity of biofilms on different pipe materials of a model drinking water distribution systems.
abstract: The experiment was conducted in three model drinking water distribution systems (DWDSs) made of unplasticized polyvinyl chloride (PVC), silane cross-linked polyethylene (PEX) and high density polyethylene (HDPE) pipes to which tap water was introduced. After 2 years of system operation, microbial communities in the DWDSs were characterized with scanning electron microscopy, heterotrophic plate count, and denaturing gradient gel electrophoresis. The most extensive biofilms were found in HDPE pipes where bacteria were either attached to mineral deposits or immersed in exopolymers. On PEX surfaces, bacteria did not form large aggregates; however, they were present in the highest number (1.24 × 10(7) cells cm(-2)). PVC biofilm did not contain mineral deposits but was made of single cells with a high abundance of Pseudomonas aeruginosa, which can be harmful to human health. The members of Proteobacteria and Bacteroidetes were found in all biofilms and the water phase. Sphingomonadales and Methylophilaceae bacteria were found only in PEX samples, whereas Geothrix fermentans, which can reduce Fe(III), were identified only in PEX biofilm. The DNA sequences closely related to the members of Alphaproteobacteria were the most characteristic and intense amplicons detected in the HDPE biofilm.
authors.affiliation: Department of Water Supply and Sewage Disposal, Faculty of Environmental Engineering, Lublin University of Technology, ul. Nadbystrzycka 40B, 20-618, Lublin, Poland, a.rozej@pollub.pl.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Rożej
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Cydzik-Kwiatkowska
authors.forename: Beata
authors.initials: B
authors.lastname: Kowalska
authors.forename: Dariusz
authors.initials: D
authors.lastname: Kowalski
mesh_terms: D001419:Bacteria / Q000145:classification* / Q000302:isolation & purification*; D044822:Biodiversity; D018441:Biofilms / Q000254:growth & development*; D015169:Colony Count, Microbial; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D058645:Denaturing Gradient Gel Electrophoresis; D060766:Drinking Water / Q000382:microbiology*; D006801:Humans; D008855:Microscopy, Electron, Scanning; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D060766:Drinking Water