title: Effect of different desensitizers on inhibition of bovine dentin demineralization: micro-computed tomography assessment.
abstract: This study evaluated the effect of two desensitizers on inhibition of dentin demineralization, after immersion in artificial saliva using micro-computed tomography (μCT). Dentin blocks cut from bovine incisors were treated with deionized water (DW, a negative control) or one of three desensitizers: a fluoride varnish (Duraphat, a positive control), a calcium phosphate desensitizer (Teethmate Desensitizer), and a fluoro-alumino-calcium silicate-based desensitizer (Nanoseal). After each treatment, the specimens in Duraphat, Nanoseal, and Teethmate Desensitizer groups were pre-immersed in artificial saliva (pH 6.5) for either 1 d or 1 wk. The mineral loss of the specimens after demineralization (pH 5.0, 3 h) was evaluated by μCT. The treated surface was investigated with scanning electron microscopy. Mineral loss in all treatment groups was significantly lower than that in DW. Duraphat was the most effective treatment against demineralization, followed by Nanoseal. Nanoseal showed significantly better reduction in mineral loss following immersion for 1 wk in artificial saliva than for 1 d. However, Teethmate Desensitizer and Duraphat did not exhibit enhanced inhibition of demineralization over a longer period of immersion in artificial saliva. Scanning electron microscopy images showed deposition of particles on the dentin in both Teethmate Desensitizer. The application of Teethmate Desensitizer and Nanoseal to the exposed dentin surface resulted in inhibition of demineralization, with Nanoseal resulting in improved inhibition after prolonged immersion in artificial saliva.
authors.affiliation: Department of Cariology and Operative Dentistry, Division of Oral Health Sciences, Graduate School of Medical and Dental Science, Tokyo Medical and Dental University (TMDU), Tokyo, Japan.
authors.forename: Ena
authors.initials: E
authors.lastname: Lodha
authors.forename: Hidenori
authors.initials: H
authors.lastname: Hamba
authors.forename: Syozi
authors.initials: S
authors.lastname: Nakashima
authors.forename: Alireza
authors.initials: A
authors.lastname: Sadr
authors.forename: Toru
authors.initials: T
authors.lastname: Nikaido
authors.forename: Junji
authors.initials: J
authors.lastname: Tagami
mesh_terms: D017607:Aluminum Compounds / Q000627:therapeutic use; D000818:Animals; D002130:Calcium Phosphates / Q000627:therapeutic use; D002327:Cariostatic Agents / Q000627:therapeutic use; D002417:Cattle; D003804:Dentin / Q000187:drug effects* / Q000648:ultrastructure; D057133:Dentin Desensitizing Agents / Q000627:therapeutic use*; D005459:Fluorides / Q000627:therapeutic use; D005460:Fluorides, Topical; D006863:Hydrogen-Ion Concentration; D007101:Immersion; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D008903:Minerals / Q000032:analysis; D053758:Nanoparticles / Q000627:therapeutic use; D012464:Saliva, Artificial / Q000737:chemistry; D017655:Silicon Compounds / Q000627:therapeutic use; D012969:Sodium Fluoride; D013997:Time Factors; D017001:Tooth Demineralization / Q000517:prevention & control*; D055114:X-Ray Microtomography / Q000379:methods*
chemical_list: D017607:Aluminum Compounds; D002130:Calcium Phosphates; D002327:Cariostatic Agents; D057133:Dentin Desensitizing Agents; D005460:Fluorides, Topical; D008903:Minerals; C588013:Nanoseal; D012464:Saliva, Artificial; D017655:Silicon Compounds; C023807:sodium fluoride topical preparation; C485830:tetracalcium phosphate; D012969:Sodium Fluoride; C485829:calcium phosphate, dibasic, anhydrous; D005459:Fluorides
keywords: demineralization; dentin; desensitizer; fluoride; micro-CT