title: Cryo-EM reveals different coronin binding modes for ADP- and ADP-BeFx actin filaments.
abstract: Essential cellular processes involving the actin cytoskeleton are regulated by auxiliary proteins that can sense the nucleotide state of actin. Here we report cryo-EM structures for ADP-bound and ADP-beryllium fluoride (ADP-BeFx, an ADP-Pi mimic)-bound actin filaments in complex with the β-propeller domain of yeast coronin 1 (crn1), at 8.6-Å resolution. Our structures reveal the main differences in the interaction of coronin with the two nucleotide states of F-actin. We derived pseudoatomic models by fitting the atomic structures of actin and coronin into the EM envelopes and confirmed the identified interfaces on actin by chemical cross-linking, fluorescence spectroscopy and actin mutagenesis. The models offer a structural explanation for the nucleotide-dependent effects of coronin on cofilin-assisted remodeling of F-actin.
authors.affiliation: 1] Department of Microbiology, Immunology and Molecular Genetics, University of California, Los Angeles (UCLA), Los Angeles, California, USA. [2] California NanoSystems Institute (CNSI), UCLA, Los Angeles, California, USA.
authors.forename: Peng
authors.initials: P
authors.lastname: Ge
authors.affiliation: Department of Chemistry and Biochemistry, UCLA, Los Angeles, California, USA.
authors.forename: Zeynep A Oztug
authors.initials: ZA
authors.lastname: Durer
authors.affiliation: Department of Chemistry and Biochemistry, Ohio State University, Columbus, Ohio, USA.
authors.forename: Dmitri
authors.initials: D
authors.lastname: Kudryashov
authors.affiliation: 1] Department of Microbiology, Immunology and Molecular Genetics, University of California, Los Angeles (UCLA), Los Angeles, California, USA. [2] California NanoSystems Institute (CNSI), UCLA, Los Angeles, California, USA. [3] Molecular Biology Institute, UCLA, Los Angeles, California, USA.
authors.forename: Z Hong
authors.initials: ZH
authors.lastname: Zhou
authors.affiliation: 1] Department of Chemistry and Biochemistry, UCLA, Los Angeles, California, USA. [2] Molecular Biology Institute, UCLA, Los Angeles, California, USA.
authors.forename: Emil
authors.initials: E
authors.lastname: Reisler
mesh_terms: D008841:Actin Cytoskeleton / Q000378:metabolism* / Q000648:ultrastructure; D000199:Actins / Q000378:metabolism* / Q000648:ultrastructure; D000244:Adenosine Diphosphate / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D001608:Beryllium / Q000378:metabolism; D020285:Cryoelectron Microscopy; D005459:Fluorides / Q000378:metabolism; D008840:Microfilament Proteins / Q000737:chemistry / Q000378:metabolism* / Q000648:ultrastructure; D008958:Models, Molecular; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D011817:Rabbits; D012441:Saccharomyces cerevisiae / Q000737:chemistry / Q000378:metabolism* / Q000648:ultrastructure
chemical_list: D000199:Actins; D008840:Microfilament Proteins; C072061:coronin proteins; C035982:beryllium fluoride; D000244:Adenosine Diphosphate; D001608:Beryllium; D005459:Fluorides