title: Downregulation of T cell immunoglobulin and mucin protein 3 in the pathogenesis of intracranial aneurysm.
abstract: Evidence has shown that inflammation acts as a critical contributor to the pathogenesis of intracranial aneurysm (IA), a potentially devastating clinical problem. T cell immunoglobulin and mucin protein 3 (Tim-3) is a negative regulatory molecule and plays important roles in the inflammation process. In the current study, we investigated the expression of Tim-3 and its correlation with tumor necrosis factor alpha (TNF-α) in IA patients. Data showed that both messenger RNA (mRNA) level and protein level of Tim-3 were significantly decreased in CD4+ T cells and CD8+ T cells from IA patients than from healthy controls (P < 0.001). However, expression of Tim-3 was not altered in monocytes between patients and healthy donors. Further analyses revealed that patients with ruptured aneurysm had significantly lower level of Tim-3 in CD8+ T cells than those with un-ruptured aneurysm. In addition, a negative correlation between serum level of TNF-α and the expression of Tim-3 in CD4+ T cells was observed in IA patients. Similar correlation was also identified in CD8+ T cells from IA patients. Our study suggests that Tim-3 may participate in the development and progression of IA by probably its negative regulation on TNF-α.
authors.affiliation: Department of Neurosurgery, General Hospital of Shenyang Military Area Command, No. 83 Wenhua Road, Shenhe District, Shenyang, 110840, China.
authors.forename: Hai-Feng
authors.initials: HF
authors.lastname: Zhang
authors.forename: Ming-Guang
authors.initials: MG
authors.lastname: Zhao
authors.forename: Guo-Biao
authors.initials: GB
authors.lastname: Liang
authors.forename: Chun-Yong
authors.initials: CY
authors.lastname: Yu
authors.forename: Zhi-Qing
authors.initials: ZQ
authors.lastname: Li
authors.forename: Xu
authors.initials: X
authors.lastname: Gao
mesh_terms: D000328:Adult; D000368:Aged; D015496:CD4-Positive T-Lymphocytes / Q000378:metabolism*; D018414:CD8-Positive T-Lymphocytes / Q000378:metabolism*; D015536:Down-Regulation / Q000502:physiology*; D005260:Female; D000072597:Hepatitis A Virus Cellular Receptor 2; D006801:Humans; D002532:Intracranial Aneurysm / Q000175:diagnosis* / Q000378:metabolism*; D008297:Male; D008565:Membrane Proteins / Q000096:biosynthesis*; D008875:Middle Aged
chemical_list: C448908:HAVCR2 protein, human; D000072597:Hepatitis A Virus Cellular Receptor 2; D008565:Membrane Proteins