title: Characteristics and response of mouse bone marrow derived novel low adherent mesenchymal stem cells acquired by quantification of extracellular matrix.
abstract: OBJECTIVE
  The aim of present study was to identify characteristic and response of mouse bone marrow (BM) derived low-adherent bone marrow mesenchymal stem cells (BMMSCs) obtained by quantification of extracellular matrix (ECM).
  
  
  METHODS
  Non-adherent cells acquired by ECM coated dishes were termed low-adherent BMMSCs and these cells were analyzed by in vitro and in vivo methods, including colony forming unit fibroblast (CFU-f), bromodeoxyuridine (BrdU), multi-potential differentiation, flow cytometry and transplantation into nude mouse to measure the bone formation ability of these low-adherent BMMSCs. Titanium (Ti) discs with machined and anodized surfaces were prepared. Adherent and low-adherent BMMSCs were cultured on the Ti discs for testing their proliferation.
  
  
  RESULTS
  The amount of CFU-f cells was significantly higher when non-adherent cells were cultured on ECM coated dishes, which was made by 7 days culturing of adherent BMMSCs. Low-adherent BMMSCs had proliferation and differentiation potential as adherent BMMSCs in vitro. The mean amount bone formation of adherent and low-adherent BMMSCs was also investigated in vivo. There was higher cell proliferation appearance in adherent and low-adherent BMMSCs seeded on anodized Ti discs than machined Ti discs by time.
  
  
  CONCLUSIONS
  Low-adherent BMMSCs acquired by ECM from non-adherent cell populations maintained potential characteristic similar to those of the adherent BMMSCs and therefore could be used effectively as adherent BMMSCs in clinic.
authors.affiliation: Department of Prosthodontics & Dental Research Institute, School of Dentistry, Seoul National University, Seoul, Republic of Korea.
authors.forename: Ri-Cheng
authors.initials: RC
authors.lastname: Zheng
authors.affiliation: Department of Prosthodontics & Dental Research Institute, Seoul National University Dental Hospital, School of Dentistry, Seoul National University, Seoul, Republic of Korea.
authors.forename: Seong-Kyun
authors.initials: SK
authors.lastname: Kim
authors.affiliation: Department of Prosthodontics & Dental Research Institute, Seoul National University Dental Hospital, School of Dentistry, Seoul National University, Seoul, Republic of Korea.
authors.forename: Seong-Joo
authors.initials: SJ
authors.lastname: Heo
authors.affiliation: Department of Prosthodontics & Dental Research Institute, Seoul National University Dental Hospital, School of Dentistry, Seoul National University, Seoul, Republic of Korea.
authors.forename: Jai-Young
authors.initials: JY
authors.lastname: Koak
authors.affiliation: Department of Prosthodontics, Asan Medical Center, College of Medicine, University of Ulsan, Seoul, Republic of Korea.
authors.forename: Joo-Hee
authors.initials: JH
authors.lastname: Lee
authors.affiliation: Department of Prosthodontics, Ewha Womans University, Seoul, Republic of Korea.
authors.forename: Ji-Man
authors.initials: JM
authors.lastname: Park
keywords: Bone formation; Bone marrow mesenchymal stem cells; Extracellular matrix; Low-adherent; Non-adherent cells; Quantification