title: [Correlation analysis of survival period and CD4+ Tcell-iATP levels in liver transplant recipients].
abstract: OBJECTIVE
  To analyze the correlation between survival time after liver transplantation and the intracellular (i)ATP levels of CD4+ T cells.
  
  
  METHODS
  Liver transplantation patients treated in our hospital,and with complete follow-up data,were enrolled retrospectively in our study (between July 2010 to October 2012) and divided into six groups according to survival time: less than 1 year,1-2 years,2-3 years,3-4 years,4-8 years,and more than 8 years.The less than 1 year survival group was further sub-divided for survival less than 1 month,1-2 months,2-3 months,3-5 months and 5-12 months.Blood samples collected from all enrollees (n=273) were analyzed by the Cylex ImmuKnow Cell Function Assay to detect the iATP level in CD4+ T cells.Correlation of iATP level in CD4+ T cells with survival time was statistically analyzed.
  
  
  RESULTS
  The levels of CD4+ T cell-iATP were significantly different among the various survival recipient groups by years: less than 1 year:325+228 ng/ml,1-2 years:216-147 ng/ml,2-3 years:225-172 ng/ml,3-5 years:236-184 ng/ml,4-8 years:298-145 ng/ml,more than 8 years:323-153 ng/ml.In addition,the levels of CD4+ T cell-iATP were significantly different between the groups of patients who survived less than 1 year: less than 1 month:441+255 ng/ml,1-2 months:357-235 ng/ml,2-3 months:353-257 ng/ml,3-5 months:202-123 ng/ml,5-12 months:234-145 ng/ml.
  
  
  CONCLUSIONS
  There is a relationship between levels of iATP in CD4+ T cells and survival time after liver transplantation.For patients with postoperative survival time of less than 1 year,the CD4+ T cell iATP level will not accurately reflect the status of CD4+ T cells' immune activation.For patients with postoperative survival time of 2-4 years,the CD4+ T cell-iATP level is relatively low and the CD4+ T lymphocyte activation status is in the low reaction zone.Patients with postoperative survival time of more than 5 years had higher CD4+ T cell-iATP level then 2-4 years survivors,and had stable CD4+ T cell immune activation.
authors.affiliation: Department of Liver Transplantation, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Qu
authors.forename: Liying
authors.initials: L
authors.lastname: Sun
authors.forename: Zhijun
authors.initials: Z
authors.lastname: Zhu
authors.forename: Ying
authors.initials: Y
authors.lastname: Liu
authors.forename: Lin
authors.initials: L
authors.lastname: Wei
authors.forename: Zhigui
authors.initials: Z
authors.lastname: Zeng
mesh_terms: D000255:Adenosine Triphosphate / Q000276:immunology; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D006085:Graft Survival; D006801:Humans; D007166:Immunosuppressive Agents; D016031:Liver Transplantation; D008213:Lymphocyte Activation; D012189:Retrospective Studies; D066027:Transplant Recipients
chemical_list: D007166:Immunosuppressive Agents; D000255:Adenosine Triphosphate