title: The Effect of Root Canal Irrigation with Combination of Sodium Hypo-chlorite and Chlorhexidine Gluconate on the Sealing Ability of Obturation Materials.
abstract: OBJECTIVE
  The aim of this study was to evaluate the effect of the precipitate that was formed by combining Sodium Hypochlorite (NaOCl) and Chlorhexidine Gluconate (CHX) on the sealing ability of root canal obturation materials.
  
  
  METHODS
  The fluid filtration method was conducted on a total of 100 roots. Samples were randomly divided into two control (n=5) and three experimental groups (n=30). The samples in group 1 were irrigated with 1.5 mL of 2.5% NaOCl, and then the smear layers of the teeth were removed by 17% EDTA, while the specimens of group 2 were irrigated by 1.5 mL of 2.5% NaOCl and 1.5 mL of 2% CHX; after the smear layer removal, a final flush with 1.5 mL of 2.5% NaOCl was performed. The samples of group 3 were irrigated the same as group 1 but after the smear layer removal canals were irrigated again with 1.5 mL of 2.5% NaOCl and then a final flush with 1.5 mL of 2% CHX was performed. Teeth were obturated with gutta-percha and AH26 sealer and after seven days, microleakage was evaluated by the fluid filtration technique. The results were analyzed by the ANOVA and Tukey's test.
  
  
  RESULTS
  The samples in group 3 had significantly greater microleakage compared to teeth in group 1, 2 (p<0.05), and the specimens in group 1 showed significantly less amount of microleakage than samples in group 2, 3 (p<0.05).
  
  
  CONCLUSIONS
  The presence of the precipitate that is formed due to interaction between NaOCl and CHX has negative effect on the sealing ability of gutta-percha and AH26 sealer.
authors.affiliation: Department of Endodontics, Dental School, Qazvin University of Medical Sciences, Qazvin, Iran.
authors.forename: Hamed
authors.initials: H
authors.lastname: Homayouni
authors.affiliation: Dental Research Laboratory, Howard University College of Dentistry, Washington DC, USA.
authors.forename: Nima Moradi
authors.initials: NM
authors.lastname: Majd
authors.affiliation: Dental Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
authors.forename: Heidar
authors.initials: H
authors.lastname: Zohrehei
authors.affiliation: Dental Carries Research Center, School of Dentistry, Qazvin University of Medical Sciences, Qazvin, Iran.
authors.forename: Behrad
authors.initials: B
authors.lastname: Mosavari
authors.affiliation: Department of Endodontics, Dental School, Qazvin University of Medical Sciences, Qazvin, Iran.
authors.forename: Mamak
authors.initials: M
authors.lastname: Adel
authors.affiliation: Department of Endodontics, Dental School, Qazvin University of Medical Sciences, Qazvin, Iran.
authors.forename: Reyhaneh
authors.initials: R
authors.lastname: Dajmar
authors.affiliation: Dental Research Center Dentistry Research Institute, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Ali
authors.initials: A
authors.lastname: Homayouni
keywords: Chlorhexidine gluconate; Fluid filtration technique; Microleakage; Para-chloroaniline; Sodium hypochlorite.