title: RNA-directed recombination of RNA in vitro.
abstract: Construction of long RNAs can be achieved in vitro by using ribozymes to recombine shorter RNAs. This can be a useful technique to prepare RNAs when the final product is either very long or contains chemical modifications that are difficult to incorporate using standard in vitro transcription techniques. Here, we describe the use of the Azoarcus group I intron ribozyme to recombine shorter RNAs into longer ones. This ribozyme is a generalized RNA recombinase ribozyme that operates rapidly and with high efficiency.
authors.affiliation: Department of Chemistry, Portland State University, 751, Portland, OR, 97207, USA, niles@pdx.edu.
authors.forename: Niles
authors.initials: N
authors.lastname: Lehman
authors.forename: Nilesh
authors.initials: N
authors.lastname: Vaidya
authors.forename: Jessica A M
authors.initials: JA
authors.lastname: Yeates
mesh_terms: D020627:Azoarcus / Q000378:metabolism*; D001483:Base Sequence; D008967:Molecular Biology / Q000379:methods*; D008969:Molecular Sequence Data; D010761:Phosphorus Radioisotopes; D012313:RNA / Q000378:metabolism*; D016337:RNA, Catalytic / Q000378:metabolism; D011995:Recombination, Genetic
chemical_list: C116167:GIR1 ribozyme; D010761:Phosphorus Radioisotopes; D016337:RNA, Catalytic; C039919:RNA, recombinant; D012313:RNA