title: Catalpol suppresses proliferation and facilitates apoptosis of OVCAR-3 ovarian cancer cells through upregulating microRNA-200 and downregulating MMP-2 expression.
abstract: Catalpol is expected to possess diverse pharmacological actions including anti-cancer, anti-inflammatory and hypoglycemic properties. Matrix metalloproteinase-2 (MMP-2) is closely related to the pathogenesis of ovarian cancer. In addition, microRNA-200 (miR-200) can modulate phenotype, proliferation, infiltration and transfer of various tumors. Here, OVCAR-3 cells were employed to investigate whether the effect of catalpol (25, 50 and 100 μg/mL) promoted apoptosis of ovarian cancer cells and to explore the potential mechanisms. Our results demonstrate that catalpol could remarkably reduce the proliferation and accelerate the apoptosis of OVCAR-3 cells. Interestingly, our findings show that catalpol treatment significantly decreased the MMP-2 protein level and increased the miR-200 expression level in OVCAR-3 cells. Further, microRNA-200 was shown to regulate the protein expression of MMP-2 in OVCAR-3 cells. It is concluded that catalpol suppressed cellular proliferation and accelerated apoptosis in OVCAR-3 ovarian cancer cells via promoting microRNA-200 expression levels and restraining MMP-2 signaling.
authors.affiliation: Department of Obstetrics and Gynecology, First Affiliated Hospital of Dalian Medical University, Dalian 116011, China. nagaomr@163.com.
authors.forename: Na
authors.initials: N
authors.lastname: Gao
authors.affiliation: Department of Obstetrics and Gynecology, First Affiliated Hospital of Dalian Medical University, Dalian 116011, China. jianxintianmr@163.com.
authors.forename: Jian-Xin
authors.initials: JX
authors.lastname: Tian
authors.affiliation: Department of Obstetrics and Gynecology, First Affiliated Hospital of Dalian Medical University, Dalian 116011, China. Yuhongshangmr@163.com.
authors.forename: Yu-Hong
authors.initials: YH
authors.lastname: Shang
authors.affiliation: Department of Oncology, Second Affiliated Hospital of Dalian Medical University, Dalian 116027, China. Danyizhaomr@163.com.
authors.forename: Dan-Yi
authors.initials: DY
authors.lastname: Zhao
authors.affiliation: Department of Oncology, Second Affiliated Hospital of Dalian Medical University, Dalian 116027, China. yha@dlmedu.edu.cn.
authors.forename: Tao
authors.initials: T
authors.lastname: Wu
mesh_terms: D017209:Apoptosis / Q000187:drug effects / Q000235:genetics; D053148:Caspase 3; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004789:Enzyme Activation / Q000187:drug effects; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects*; D006801:Humans; D057888:Iridoid Glucosides / Q000737:chemistry / Q000494:pharmacology*; D020778:Matrix Metalloproteinase 2 / Q000235:genetics*; D035683:MicroRNAs / Q000235:genetics*; D010051:Ovarian Neoplasms / Q000235:genetics* / Q000378:metabolism; D034622:RNA Interference; D012333:RNA, Messenger / Q000235:genetics
chemical_list: D057888:Iridoid Glucosides; C533896:MIRN200 microRNA, human; D035683:MicroRNAs; D012333:RNA, Messenger; C078040:catalpol; D053148:Caspase 3; D020778:Matrix Metalloproteinase 2