title: siRNA-mediated knockdown against NUF2 suppresses pancreatic cancer proliferation in vitro and in vivo.
abstract: NUF2 (NUF2, Ndc80 kinetochore complex component) plays an important role in kinetochore-microtubule attachment. It has been reported that NUF2 is associated with multiple human cancers. However, the functional role of NUF2 in pancreatic cancer remains unclear. In this study, we found that NUF2 expression was stronger in tumour tissues than in normal pancreatic tissues, and its overexpression could be related to poor prognosis. Moreover, NUF2 was highly expressed in several human pancreatic cancer cell lines. We took advantage of lentivirus-mediated siRNA (small interfering RNA) to suppress NUF2 expression in PANC-1 and Sw1990 cell lines aiming to investigate the role of NUF2 in pancreatic cancer. NUF2 silencing by RANi (RNA interference) reduced the proliferation and colony formation ability of pancreatic cancer cells in vitro. Cell cycle analysis showed that NUF2 knockdown induced cell cycle arrest at G0/G1 phase via suppression of Cyclin B1, Cdc2 and Cdc25A. More importantly, NUF2 silencing was able to alleviate in vivo tumourigenesis in pancreatic cancer xenograft nude mice. Collectively, the present study indicates that the siRNA-mediated knockdown against NUF2 may be a promising therapeutic method for the treatment of pancreatic cancer.
authors.affiliation: *Department of Hepatobiliary Surgery, Southwest Hospital, Third Military Medical University, ChongQing, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Hu
authors.affiliation: *Department of Hepatobiliary Surgery, Southwest Hospital, Third Military Medical University, ChongQing, China.
authors.forename: Xi
authors.initials: X
authors.lastname: Chen
authors.affiliation: *Department of Hepatobiliary Surgery, Southwest Hospital, Third Military Medical University, ChongQing, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Sun
authors.affiliation: *Department of Hepatobiliary Surgery, Southwest Hospital, Third Military Medical University, ChongQing, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Bie
authors.affiliation: *Department of Hepatobiliary Surgery, Southwest Hospital, Third Military Medical University, ChongQing, China.
authors.forename: Lei-Da
authors.initials: LD
authors.lastname: Zhang
mesh_terms: D000818:Animals; D002453:Cell Cycle; D018797:Cell Cycle Proteins / Q000235:genetics*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D010179:Pancreas / Q000378:metabolism / Q000473:pathology; D010190:Pancreatic Neoplasms / Q000235:genetics* / Q000473:pathology / Q000628:therapy*; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics* / Q000627:therapeutic use; D000067492:RNAi Therapeutics / Q000379:methods
chemical_list: D018797:Cell Cycle Proteins; C490197:NUF2 protein, human; D034741:RNA, Small Interfering