title: Poly(2-vinyl-4,4-dimethylazlactone)-functionalized magnetic nanoparticles as carriers for enzyme immobilization and its application.
abstract: Fabrication of various efficient enzyme reactors has triggered increasing interests for its extensive applications in biological and clinical research. In this study, magnetic nanoparticles were functionalized by a biocompatible reactive polymer, poly(2-vinyl-4,4-dimethylazlactone), which was synthesized by reversible addition-fragmentation chain transfer polymerization. Then, the prepared polymer-modified magnetic nanoparticles were employed as favorable carriers for enzyme immobilization. l-Asparaginase was selected as the model enzyme to fabricate the enzyme reactor, and the prepared enzyme reactor exhibited high loading capacity of 318.0 μg mg(-1) magnetic nanoparticle. Interestingly, it has been observed that the enzymolysis efficiency increased slightly with the lengthened polymer chain, resulting from the increased immobilization amount of enzyme. Meanwhile, the immobilized enzyme could retain more than 95.7% activity after 10 repeated uses and maintain more than 72.6% activity after 10 weeks storage. Moreover, an extracorporeal shunt system was simulated to estimate the potential application capability of the prepared l-asparaginase reactor in acute lymphoblastic leukemia treatment.
authors.affiliation: Beijing National Laboratory for Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, Chinese Academy of Sciences , Beijing 100190, P.R. China.
authors.forename: Xiaoyu
authors.initials: X
authors.lastname: Mu
authors.forename: Juan
authors.initials: J
authors.lastname: Qiao
authors.forename: Li
authors.initials: L
authors.lastname: Qi
authors.forename: Ping
authors.initials: P
authors.lastname: Dong
authors.forename: Huimin
authors.initials: H
authors.lastname: Ma
mesh_terms: D001215:Asparaginase / Q000008:administration & dosage / Q000737:chemistry; D019149:Bioreactors; D004800:Enzymes, Immobilized / Q000008:administration & dosage / Q000737:chemistry*; D006801:Humans; D007783:Lactones / Q000737:chemistry*; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D011145:Polyvinyls / Q000737:chemistry*; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000188:drug therapy*
chemical_list: D004800:Enzymes, Immobilized; D007783:Lactones; D058185:Magnetite Nanoparticles; D011145:Polyvinyls; C539182:poly(2-vinyl-4,4-dimethyl azlactone); D001215:Asparaginase
keywords: enzyme immobilization; l-asparaginase; magnetic nanoparticles; poly(2-vinyl-4,4-dimethylazlactone)