title: Sub-diffusive scattering parameter maps recovered using wide-field high-frequency structured light imaging.
abstract: This study investigates the hypothesis that structured light reflectance imaging with high spatial frequency patterns [Formula: see text] can be used to quantitatively map the anisotropic scattering phase function distribution [Formula: see text] in turbid media. Monte Carlo simulations were used in part to establish a semi-empirical model of demodulated reflectance ([Formula: see text]) in terms of dimensionless scattering [Formula: see text] and [Formula: see text], a metric of the first two moments of the [Formula: see text] distribution. Experiments completed in tissue-simulating phantoms showed that simultaneous analysis of [Formula: see text] spectra sampled at multiple [Formula: see text] in the frequency range [0.05-0.5] [Formula: see text] allowed accurate estimation of both [Formula: see text] in the relevant tissue range [0.4-1.8] [Formula: see text], and [Formula: see text] in the range [1.4-1.75]. Pilot measurements of a healthy volunteer exhibited [Formula: see text]-based contrast between scar tissue and surrounding normal skin, which was not as apparent in wide field diffuse imaging. These results represent the first wide-field maps to quantify sub-diffuse scattering parameters, which are sensitive to sub-microscopic tissue structures and composition, and therefore, offer potential for fast diagnostic imaging of ultrastructure on a size scale that is relevant to surgical applications.
authors.affiliation: Thayer School of Engineering, Dartmouth College, Hanover, NH, 03755 USA.
authors.forename: Stephen Chad
authors.initials: SC
authors.lastname: Kanick
authors.affiliation: Thayer School of Engineering, Dartmouth College, Hanover, NH, 03755 USA.
authors.forename: David M
authors.initials: DM
authors.lastname: McClatchy
authors.affiliation: Thayer School of Engineering, Dartmouth College, Hanover, NH, 03755 USA.
authors.forename: Venkataramanan
authors.initials: V
authors.lastname: Krishnaswamy
authors.affiliation: Thayer School of Engineering, Dartmouth College, Hanover, NH, 03755 USA.
authors.forename: Jonathan T
authors.initials: JT
authors.lastname: Elliott
authors.affiliation: Thayer School of Engineering, Dartmouth College, Hanover, NH, 03755 USA.
authors.forename: Keith D
authors.initials: KD
authors.lastname: Paulsen
authors.affiliation: Thayer School of Engineering, Dartmouth College, Hanover, NH, 03755 USA.
authors.forename: Brian W
authors.initials: BW
authors.lastname: Pogue
keywords: (170.3660) Light propagation in tissues; (170.3880) Medical and biological imaging; (170.6510) Spectroscopy, tissue diagnostics; (170.7050) Turbid media; (290.0290) Scattering