title: Glycyrrhetinic Acid inhibits cell growth and induces apoptosis in ovarian cancer a2780 cells.
abstract: OBJECTIVE
  Accumulating evidence indicates that glycyrrhizin (GZ) and its hydrolyzed metabolite 18-β glycyrrhetinic acid (GA) exhibit anti-inflammatory and anticancer activities. The objective of this study was to examine the in vitro cytotoxic activity of GA on human ovarian cancer A2780 cells.
  
  
  METHODS
  A2780 cells were cultured in RPMI1640 containing 10% fetal bovine serum. Cells were treated with different doses of GA and cell viability and proliferation were detected by dye exclusion and 3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide (XTT) assays. Apoptosis induction and expression of Fas and Fas ligand (FasL) were analyzed by flow cytometry.
  
  
  RESULTS
  We observed that GA decreases cell viability and suppressed cells proliferation in a dose-dependent manner as detected by dye-exclusion and XTT assayes. In addition, our flow cytometry data show that GA not only induces apoptosis in A2780 cells but also upregulates both Fas and FasL on these cells in a dose-dependent manner.
  
  
  CONCLUSIONS
  we demonstrate that GA causes cell death in A2780 cells by inducing apoptosis.
authors.affiliation: Department of Biochemistry, Research and Science, Islamic Azad University, Sanandaj, Iran.
authors.forename: Venus
authors.initials: V
authors.lastname: Haghshenas
authors.affiliation: Kurdistan Cellular and Molecular Research Center, Kurdistan University of Medical Sciences, Sanandaj, Iran.
authors.forename: Shohreh
authors.initials: S
authors.lastname: Fakhari
authors.affiliation: Department of Biochemistry, Sanandaj Branch, Islamic Azad University, Iran.
authors.forename: Sako
authors.initials: S
authors.lastname: Mirzaie
authors.affiliation: Kurdistan Cellular and Molecular Research Center, Kurdistan University of Medical Sciences, Sanandaj, Iran.
authors.forename: Mohammadreza
authors.initials: M
authors.lastname: Rahmani
authors.affiliation: Department of Gynecology, Faculty of Medicine, Kurdistan University of Medical Sciences, Sanandaj, Iran.
authors.forename: Fariba
authors.initials: F
authors.lastname: Farhadifar
authors.affiliation: Department of Biochemistry, Research and Science, Islamic Azad University, Sanandaj, Iran.
authors.forename: Sara
authors.initials: S
authors.lastname: Pirzadeh
authors.affiliation: Kurdistan Cellular and Molecular Research Center, Kurdistan University of Medical Sciences, Sanandaj, Iran.
authors.forename: Ali
authors.initials: A
authors.lastname: Jalili
keywords: Apoptosis; Glycyrrhetinic acid; Ovarian Cancer