title: Rotavirus G2P[4] detection in fresh vegetables and oysters in Mexico City.
abstract: Rotaviruses are the principal cause of dehydration caused by diarrhea in children younger than 2 years of age. Although these viral infections have mainly been associated with ingestion of fecally contaminated food and water, few studies have addressed the presence of the virus in food that is consumed raw or slightly cooked. In this work, 30 oyster samples and 33 vegetable samples were examined for the presence of rotavirus genotypes to evaluate their potential to produce gastrointestinal infections. The rotaviruses were identified by reverse transcriptase PCR amplification of the VP7 gene. G and P genotyping was also performed by reverse transcriptase PCR, with a detection sensitivity of up to 15 PFU/ml. Rotaviruses were found in 17 (26.9%) of 63 samples (10 oysters and 7 vegetables). The G2 genotype was found in 11 (64.7%) of 17 of the rotavirus strains, and 16 (94.1%) of 17 had the P[4] genotype. The combined genotypes found most frequently were G2P[4] (10 [58.82%] of 17), GNTP[4] (6 [35.29%] of 17), and G2P[NT] (1 [5.8%] of 17).
authors.affiliation: Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, 11340 Mexico D.F., Mexico.
authors.forename: Carolina
authors.initials: C
authors.lastname: Quiroz-Santiago
authors.affiliation: Departamento de Biotecnología, Ciencias Biológicas y de la Salud, Universidad Autónoma Metropolitana-Iztapalapa, 09340 Mexico D.F., Mexico.
authors.forename: Carlos
authors.initials: C
authors.lastname: Vázquez-Salinas
authors.affiliation: Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, 11340 Mexico D.F., Mexico.
authors.forename: Ivan
authors.initials: I
authors.lastname: Natividad-Bonifacio
authors.affiliation: Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, 11340 Mexico D.F., Mexico.
authors.forename: Blanca Lilia
authors.initials: BL
authors.lastname: Barrón-Romero
authors.affiliation: Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, 11340 Mexico D.F., Mexico. elsairma46@yahoo.com.mx.
authors.forename: Elsa Irma
authors.initials: EI
authors.lastname: Quiñones-Ramírez
mesh_terms: D000818:Animals; D005506:Food Contamination / Q000032:analysis* / Q000706:statistics & numerical data; D005838:Genotype; D008800:Mexico; D010125:Ostreidae / Q000821:virology*; D012401:Rotavirus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D012400:Rotavirus Infections / Q000382:microbiology; D012758:Shellfish / Q000032:analysis / Q000821:virology*; D014675:Vegetables / Q000821:virology*