title: Two-step procedure for evaluating experimentally induced DNA damage: Texas Red and Comet assays.
abstract: The technique of Texas Red immunostaining for cellular γH2AX identifies by fluorescence microscopy DNA undergoing active remodeling or repair. To fully characterize γH2AX foci, the technique of alkaline single cell electrophoresis (Comet) assay quantifiably resolves DNA double-strand breaks from other types of DNA damage. When used together, these two techniques may provide evidence for radiochemotherapy-induced DNA damage.
authors.affiliation: Department of Radiation Oncology, Case Western Reserve University, Cleveland, OH, USA.
authors.forename: Gina J
authors.initials: GJ
authors.lastname: Ferris
authors.forename: Lauren B
authors.initials: LB
authors.lastname: Shunkwiler
authors.forename: Charles A
authors.initials: CA
authors.lastname: Kunos
mesh_terms: D045744:Cell Line, Tumor; D020552:Comet Assay / Q000379:methods*; D004249:DNA Damage; D006657:Histones / Q000378:metabolism; D006801:Humans; D007091:Image Processing, Computer-Assisted; D014966:Xanthenes / Q000378:metabolism*
chemical_list: C468783:H2AX protein, human; D006657:Histones; D014966:Xanthenes; C034657:Texas red