title: High-density array comparative genomic hybridization detects novel copy number alterations in gastric adenocarcinoma.
abstract: OBJECTIVE
  To investigate frequent quantitative alterations of intestinal-type gastric adenocarcinoma.
  
  
  METHODS
  We analyzed genome-wide DNA copy numbers of 22 samples and using CytoScan® HD Array.
  
  
  RESULTS
  We identified 22 gene alterations that to the best of our knowledge have not been described for gastric cancer, including of v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 4 (ERBB4), SRY (sex determining region Y)-box 6 (SOX6), regulator of telomere elongation helicase 1 (RTEL1) and UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 5 (B4GALT5). The most significant alterations related to peritoneal invasion involved the regions 13q21.1 (gain) and 15q15.1, 17q23.1, 19q13.2 and 20q11.22 (loss of heterozygozity; LOH), where we found LOH of erythrocyte membrane protein band 4.1-like 1 (EPB41L1) gene. In relation to early age of onset, the most significant alterations were gains in the regions Xq26 and Xp22.31 and a loss in the region 11p15.4.
  
  
  CONCLUSIONS
  These quantitative changes may play a role in the development of this type of neoplasia and may be used as markers in evaluating poor prognosis, as well as act as potential therapeutic targets for gastric cancer.
authors.affiliation: Human Cytogenetics Laboratory, Federal University of Pará, Belém Pará, Brazil Oncology Research Center, Federal University of Pará, Belém Pará, Brazil.
authors.forename: Aline Damasceno
authors.initials: AD
authors.lastname: Seabra
authors.affiliation: Human Cytogenetics Laboratory, Federal University of Pará, Belém Pará, Brazil Oncology Research Center, Federal University of Pará, Belém Pará, Brazil.
authors.forename: Taíssa Maíra Thomaz
authors.initials: TM
authors.lastname: Araújo
authors.affiliation: Human Cytogenetics Laboratory, Federal University of Pará, Belém Pará, Brazil Oncology Research Center, Federal University of Pará, Belém Pará, Brazil.
authors.forename: Fernando Augusto Rodrigues
authors.initials: FA
authors.lastname: Mello Junior
authors.affiliation: Human Cytogenetics Laboratory, Federal University of Pará, Belém Pará, Brazil Oncology Research Center, Federal University of Pará, Belém Pará, Brazil.
authors.forename: Diego
authors.initials: D
authors.lastname: Di Felipe Ávila Alcântara
authors.affiliation: Human Cytogenetics Laboratory, Federal University of Pará, Belém Pará, Brazil Oncology Research Center, Federal University of Pará, Belém Pará, Brazil.
authors.forename: Amanda Paiva
authors.initials: AP
authors.lastname: De Barros
authors.affiliation: Oncology Research Center, Federal University of Pará, Belém Pará, Brazil.
authors.forename: Paulo Pimentel
authors.initials: PP
authors.lastname: De Assumpção
authors.affiliation: Human Cytogenetics Laboratory, Federal University of Pará, Belém Pará, Brazil Oncology Research Center, Federal University of Pará, Belém Pará, Brazil.
authors.forename: Raquel Carvalho
authors.initials: RC
authors.lastname: Montenegro
authors.affiliation: Human Cytogenetics Laboratory, Federal University of Pará, Belém Pará, Brazil Oncology Research Center, Federal University of Pará, Belém Pará, Brazil.
authors.forename: Adriana Costa
authors.initials: AC
authors.lastname: Guimarães
authors.affiliation: Oncology Research Center, Federal University of Pará, Belém Pará, Brazil.
authors.forename: Samia
authors.initials: S
authors.lastname: Demachki
authors.affiliation: Human Cytogenetics Laboratory, Federal University of Pará, Belém Pará, Brazil Oncology Research Center, Federal University of Pará, Belém Pará, Brazil.
authors.forename: Rommel Mario Rodríguez
authors.initials: RM
authors.lastname: Burbano
authors.affiliation: Human Cytogenetics Laboratory, Federal University of Pará, Belém Pará, Brazil Oncology Research Center, Federal University of Pará, Belém Pará, Brazil khayatas@gmail.com.
authors.forename: André Salim
authors.initials: AS
authors.lastname: Khayat
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics* / Q000473:pathology; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000235:genetics*; D043171:Chromosomal Instability; D002877:Chromosomes, Human / Q000235:genetics*; D055028:Comparative Genomic Hybridization / Q000379:methods*; D056915:DNA Copy Number Variations / Q000235:genetics*; D004273:DNA, Neoplasm / Q000235:genetics; D005260:Female; D006801:Humans; D007414:Intestinal Neoplasms / Q000235:genetics / Q000473:pathology; D019656:Loss of Heterozygosity; D008297:Male; D018895:Microsatellite Repeats; D008875:Middle Aged; D009367:Neoplasm Staging; D011379:Prognosis; D013274:Stomach Neoplasms / Q000235:genetics* / Q000473:pathology
chemical_list: D014408:Biomarkers, Tumor; D004273:DNA, Neoplasm
keywords: Gastric adenocarcinoma; aCGH; carcinogenesis; intestinal type