title: Hydrogen sulfide augments survival signals in warm ischemia and reperfusion of the mouse liver.
abstract: OBJECTIVE
  Hydrogen sulfide (H2S) ameliorates hepatic ischemia and reperfusion injury (IRI), but the precise mechanism remains elusive. We investigated whether sodium hydrogen sulfide (NaHS), a soluble derivative of H2S, would ameliorate hepatic IRI, and if so, via what mechanism.
  
  
  METHODS
  Mice were subjected to partial warm ischemia for 75 min followed by reperfusion. Either NaHS or saline was administered intravenously 10 min before reperfusion. The liver and serum were collected 3, 6, and 24 h after reperfusion.
  
  
  RESULTS
  In the NaHS(-) group, severe IRI was apparent by the ALT leakage, tissue injury score, apoptosis, lipid peroxidation, and inflammation (higher plasma TNF-α, IL-6, IL-1β, IFN-γ, IL-23, IL-17, and CD40L), whereas IRI was significantly ameliorated in the NaHS(+) group. These effects could be explained by the augmented nuclear translocation of Nrf2, and the resulting up-regulation of HO-1 and thioredoxin-1. Phosphorylation of the PDK-1/Akt/mTOR/p70S6k axis, which is known to mediate pro-survival and anti-apoptotic signals, was significantly augmented in the NaHS(+) group, with a higher rate of PCNA-positive cells thereafter.
  
  
  CONCLUSIONS
  NaHS ameliorated hepatic IRI by direct and indirect anti-oxidant activities by augmenting pro-survival, anti-apoptotic, and anti-inflammatory signals via mechanisms involving Nrf-2, and by accelerating hepatic regeneration via mechanisms involving Akt-p70S6k.
authors.affiliation: Department of Gastroenterological Surgery I, Graduate School of Medicine, Hokkaido University, N-15, W-7, Kita-ku, Sapporo, 060-8638, Japan.
authors.forename: Shingo
authors.initials: S
authors.lastname: Shimada
authors.forename: Moto
authors.initials: M
authors.lastname: Fukai
authors.forename: Kenji
authors.initials: K
authors.lastname: Wakayama
authors.forename: Takahisa
authors.initials: T
authors.lastname: Ishikawa
authors.forename: Nozomi
authors.initials: N
authors.lastname: Kobayashi
authors.forename: Taichi
authors.initials: T
authors.lastname: Kimura
authors.forename: Kenichiro
authors.initials: K
authors.lastname: Yamashita
authors.forename: Toshiya
authors.initials: T
authors.lastname: Kamiyama
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Shimamura
authors.forename: Akinobu
authors.initials: A
authors.lastname: Taketomi
authors.forename: Satoru
authors.initials: S
authors.lastname: Todo
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D015415:Biomarkers / Q000378:metabolism; D015153:Blotting, Western; D016207:Cytokines / Q000378:metabolism*; D007150:Immunohistochemistry; D008099:Liver / Q000098:blood supply* / Q000187:drug effects / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018384:Oxidative Stress / Q000187:drug effects; D020011:Protective Agents / Q000494:pharmacology / Q000627:therapeutic use*; D015424:Reperfusion / Q000009:adverse effects*; D015427:Reperfusion Injury / Q000209:etiology / Q000378:metabolism / Q000517:prevention & control*; D013440:Sulfides / Q000494:pharmacology / Q000627:therapeutic use*; D052096:Warm Ischemia / Q000009:adverse effects*
chemical_list: D015415:Biomarkers; D016207:Cytokines; D020011:Protective Agents; D013440:Sulfides; C025451:sodium bisulfide