title: Molecular instability induced by aluminum stress in Plantago species.
abstract: Aluminum (Al) is one of the most abundant metals on earth's crust and Al toxicity represents one of the major factors that limit plant growth and productivity in acid soils (with a pH≤5.0). In this study the mutagenic/genotoxic effects of Al were evaluated in roots and leaves of two Plantago, species, Plantago almogravensis and Plantago lagopus, using ISSRs markers. Both species were exposed to 400 μM Al during 7 and 21 days. Ten ISSR primers produced polymorphic bands. In P. almogravensis, a total of 257 and 258 bands in roots and 255 and 265 bands in leaves were produced in the presence and absence of Al, respectively. In P. lagopus were produced 279 and 278 a total bands in roots and 275 and 274 bands in leaves, under the same conditions. The changes in ISSR profiles after Al treatment were considered as gain and/or loss of bands compared with the controls. The results suggest that changes in genomic template stability (GTS) could be detected with ISSR profiles. This molecular marker proved to be a good tool to detect the effects of Al on DNA profiles. It seems that Al did not interfere significantly with DNA integrity in both species but generated less ISSR stability in P. almogravensis than in P. lagopus. The results confirm the tolerance of P. almogravensis and suggest the same behavior of P. lagopus. Although further studies are required for confirmation the Al tolerance behavior of P. lagopus, a potential application for phytoremediation can be also considered due its wide distribution.
authors.affiliation: Institute of Biotechnology and Bioengineering, Centre of Genomics and Biotechnology, University of Trás-os-Montes and Alto Douro (IBB/CGB-UTAD), P.O. Box 1013, 5000-801 Vila Real, Portugal. Electronic address: sofiammcorreia@gmail.com.
authors.forename: Sofia
authors.initials: S
authors.lastname: Correia
authors.affiliation: Institute of Biotechnology and Bioengineering, Centre of Genomics and Biotechnology, University of Trás-os-Montes and Alto Douro (IBB/CGB-UTAD), P.O. Box 1013, 5000-801 Vila Real, Portugal.
authors.forename: Manuela
authors.initials: M
authors.lastname: Matos
authors.affiliation: Institute of Biotechnology and Bioengineering, Centre of Genomics and Biotechnology, University of Trás-os-Montes and Alto Douro (IBB/CGB-UTAD), P.O. Box 1013, 5000-801 Vila Real, Portugal.
authors.forename: Vanessa
authors.initials: V
authors.lastname: Ferreira
authors.affiliation: Institute of Biotechnology and Bioengineering, Centre of Genomics and Biotechnology (IBB/CGB), Faculty of Sciences and Technology, University of Algarve, Gambelas Campus, Ed. 8, 8005-139 Faro, Portugal.
authors.forename: Neusa
authors.initials: N
authors.lastname: Martins
authors.affiliation: Institute of Biotechnology and Bioengineering, Centre of Genomics and Biotechnology (IBB/CGB), Faculty of Sciences and Technology, University of Algarve, Gambelas Campus, Ed. 8, 8005-139 Faro, Portugal.
authors.forename: Sandra
authors.initials: S
authors.lastname: Gonçalves
authors.affiliation: Institute of Biotechnology and Bioengineering, Centre of Genomics and Biotechnology (IBB/CGB), Faculty of Sciences and Technology, University of Algarve, Gambelas Campus, Ed. 8, 8005-139 Faro, Portugal.
authors.forename: Anabela
authors.initials: A
authors.lastname: Romano
authors.affiliation: Institute of Biotechnology and Bioengineering, Centre of Genomics and Biotechnology, University of Trás-os-Montes and Alto Douro (IBB/CGB-UTAD), P.O. Box 1013, 5000-801 Vila Real, Portugal.
authors.forename: Olinda
authors.initials: O
authors.lastname: Pinto-Carnide
mesh_terms: D000535:Aluminum / Q000633:toxicity*; D001673:Biodegradation, Environmental / Q000187:drug effects; D004249:DNA Damage / Q000187:drug effects*; D018744:DNA, Plant / Q000302:isolation & purification; D042822:Genomic Instability / Q000187:drug effects; D018515:Plant Leaves / Q000187:drug effects; D018517:Plant Roots / Q000187:drug effects*; D010943:Plantago / Q000737:chemistry / Q000187:drug effects* / Q000235:genetics
chemical_list: D018744:DNA, Plant; D000535:Aluminum
keywords: Aluminum; Genomic template stability (GTS); Genotoxic effects; Inter Simple Sequence Repeat (ISSR); Plantago