title: Distinct allelic expression patterns of imprintedIGF2in adenocarcinoma and squamous cell carcinoma of the lung.
abstract: The insulin-like growth factor 2 gene (IGF2) is an imprinting gene, which mediates cell growth and apoptosis. The loss of imprinting (LOI) ofIGF2has been associated with the development of cancer. In the present study, loss LOI ofIGF2in lung cancer was analyzed using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) in combination with DNA sequencing of samples collected by laser capture microdissection. The status of each sample was assigned as imprinting when PCR-RFLP revealed only one band or sequence with a single peak; otherwise, the case was classified as LOI. LOI was identified in eight out of 13 adenocarcinoma cases (62%), but was not detected in any of the nine squamous cell carcinoma cases (0%). These results suggest thatIGF2LOI is involved in the molecular pathogenesis of lung adenocarcinoma, but not squamous cell carcinoma, and that LOI may be detected through increasedIGF2expression levels.
authors.affiliation: Department of Clinical Laboratory Science, Kanazawa University, Ishikawa 920-0942, Japan.
authors.forename: Satoru
authors.initials: S
authors.lastname: Ozaki
authors.affiliation: Department of Clinical Laboratory Science, Kanazawa University, Ishikawa 920-0942, Japan.
authors.forename: Ei
authors.initials: E
authors.lastname: Kawahara
authors.affiliation: Department of Clinical Laboratory Science, Kanazawa University, Ishikawa 920-0942, Japan.
authors.forename: Shiori
authors.initials: S
authors.lastname: Maenaka
authors.affiliation: Department of Clinical Laboratory Science, Kanazawa University, Ishikawa 920-0942, Japan.
authors.forename: Nguyen Viet
authors.initials: NV
authors.lastname: Hoang
authors.affiliation: Department of Pathology, Kanazawa University, Ishikawa 920-0942, Japan.
authors.forename: Takeru
authors.initials: T
authors.lastname: Oyama
authors.affiliation: Department of Health Science, Ishikawa Prefectural Nursing University, Ishikawa 929-1212, Japan.
authors.forename: Miwa
authors.initials: M
authors.lastname: Imai
authors.affiliation: Department of Lung Surgery, Kanazawa University Hospital, Ishikawa 920-8641, Japan.
authors.forename: Makoto
authors.initials: M
authors.lastname: Oda
authors.affiliation: Department of Internal Medicine, Kanazawa University Cancer Research Institute, Ishikawa 920-1192, Japan.
authors.forename: Seiji
authors.initials: S
authors.lastname: Yano
keywords: adenocarcinoma; genomic imprinting; insulin-like growth factor 2; lung cancer; microdissection