title: A comparison of infectivity between polyhedra of the Spodoptera litura multiple nucleopolyhedrovirus before and after passage through the gut of the stink bug, Eocanthecona furcellata.
abstract: Infectivity of polyhedra of Spodoptera litura multiple nucleopolyhedrovirus before and after passage through the gut of the predatory stink bug, Eocanthecona furcellata Wolff (Hemiptera: Pentatomidae) was compared through field bioassay studies. Three sets of E. furcellata were used for bioassays and these were allowed to feed on a single meal of five third instar Oriental leaf worm, Spodoptera litura (Fabricius) (Lepidoptera: Noctuidae), that were infected with polyhedra before passage, after passage, and healthy (control) larvae 1 day prior to the trial. The predators were subsequently released on cabbage plants that were infested with 100 healthy S. litura larvae. The median lethal dose (LD50) and survival time (ST50) values before and after passage through the gut were not significantly different. Additional mortality due to virus infection increased 13- 17% before and after treatments but within these treatments the mortality did not vary significantly. It was concluded that E. furcellata disseminated the virus through their feces into the ecosystem and infectivity of the SpltMNPV was not altered after passage through the gut of the predator.
authors.affiliation: 1Division of Entomology, Sher-e-Kashmir University of Agricultural Sciences and Technology-Jammu, Chatha, Jammu and Kashmir, India - 180009 rkguptaentoskuast@gmail.com.
authors.forename: R K
authors.initials: RK
authors.lastname: Gupta
authors.affiliation: 1Division of Entomology, Sher-e-Kashmir University of Agricultural Sciences and Technology-Jammu, Chatha, Jammu and Kashmir, India - 180009 mudasir32@gmail.com.
authors.forename: Mudasir
authors.initials: M
authors.lastname: Gani
authors.affiliation: 2GLBRC Research Coordinator, W.K. Kellogg Biological Station; Michigan State University; 3700 E Gull Lake Dr Hickory Corners, MI 49060 poonamjasrotia@gmail.com.
authors.forename: P
authors.initials: P
authors.lastname: Jasrotia
authors.affiliation: 1Division of Entomology, Sher-e-Kashmir University of Agricultural Sciences and Technology-Jammu, Chatha, Jammu and Kashmir, India - 180009 kuldeep.ipm@gmail.com.
authors.forename: K
authors.initials: K
authors.lastname: Srivastava
authors.affiliation: 1Division of Entomology, Sher-e-Kashmir University of Agricultural Sciences and Technology-Jammu, Chatha, Jammu and Kashmir, India - 180009 kaulvirender@gmail.com.
authors.forename: V
authors.initials: V
authors.lastname: Kaul
mesh_terms: D000818:Animals; D005260:Female; D020387:Food Chain; D020062:Heteroptera; D054884:Host-Pathogen Interactions; D017924:Nucleopolyhedroviruses / Q000472:pathogenicity*; D018411:Spodoptera / Q000821:virology*
keywords: LD50; ST50; SpltMNPV after passage; SpltMNPV before passage