title: Method validation of a tricyclic antidepressant drug panel in urine by UPLC-MS/MS.
abstract: Tricyclic antidepressant (TCA) drugs are also used as adjunctive therapy to treat chronic pain. To monitor patient compliance to therapy, urine specimens may be preferred since collection is non-invasive, and the specimen can provide a longer detection window. TCA drugs are frequently monitored by immunoassay; however, poor antibody specificity may compromise results. The purpose for this study was to develop a confirmation method for determining TCA in urine specimens by ultra-pressure liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). Our method can quantify 9 TCA drugs in less than 5 min. Method validation experiments were performed, and the coefficient variation for inter- and intra-day precision was within 12% for each analyte at five different concentrations. Accuracy studies had good agreement with another laboratory that performs testing by GC/MS.
  
  
  CONCLUSIONS
  1. Tricyclic antidepressants are commonly used to treat depression, anxiety, and neuropathic pain. 2. A confirmation method by ultra-pressure liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) to determine TCA in urine specimens was developed. 3. Urine testing for TCA is preferred for monitoring adherence to therapy due to ease of collection.
authors.affiliation: Department of Laboratory Medicine, Chang-Gung Memorial Hospital, Taoyuan, Taiwan Department of Pathology, University of Utah Health Sciences Center, Salt Lake City, UT.
authors.forename: Chia-Ni
authors.initials: CN
authors.lastname: Lin
authors.affiliation: ARUP Institute for Clinical and Experimental Pathology, Salt Lake City, UT, United States.
authors.forename: JoEtta M
authors.initials: JM
authors.lastname: Juenke
authors.affiliation: Department of Pathology, University of Utah Health Sciences Center, Salt Lake City, UT ARUP Institute for Clinical and Experimental Pathology, Salt Lake City, UT, United States kamisha.johnson-davis@aruplab.com.
authors.forename: Kamisha L
authors.initials: KL
authors.lastname: Johnson-Davis
mesh_terms: D000929:Antidepressive Agents, Tricyclic / Q000627:therapeutic use / Q000652:urine*; D002851:Chromatography, High Pressure Liquid; D003863:Depression / Q000188:drug therapy / Q000652:urine; D006801:Humans; D007118:Immunoassay; D016014:Linear Models; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry; D016482:Urinalysis
chemical_list: D000929:Antidepressive Agents, Tricyclic
keywords: Tricyclic antidepressants (TCA); UPLC-MS/MS; Urine drug testing