title: Siliceous mesostructured cellular foams/poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) composite biomaterials for bone regeneration.
abstract: Osteoinductive and biodegradable composite biomaterials for bone regeneration were prepared by combining poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (PHBHHx) with siliceous mesostructured cellular foams (SMC), using the porogen leaching method. Surface hydrophilicity, morphology, and recombinant human bone morphogenetic protein 2 adsorption/release behavior of the SMC/PHBHHx scaffolds were analyzed. Results of scanning electron microscopy indicated that the SMC was uniformly dispersed in the PHBHHx scaffolds, and SMC modification scaffolds have an interconnected porous architecture with pore sizes ranging from 200 to 400 μm. The measurements of the water contact angles suggested that the incorporation of SMC into PHBHHx improves the hydrophilicity of the composite. In vitro studies with simulated body fluid show great improvements to bioactivity and biodegradability versus pure PHBHHx scaffolds. Cell adhesion and cell proliferation on the scaffolds was also evaluated, and the new tools provide a better environment for human mesenchymal stem cell attachment, spreading, proliferation, and osteogenic differentiation on PHBHHx scaffolds. Moreover, micro-computed tomography and histological evaluation confirmed that the SMC/PHBHHx scaffolds improved the efficiency of new bone regeneration with excellent biocompatibility and biodegradability and faster and more effective osteogenesis in vivo.
authors.affiliation: The State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, People's Republic of China.
authors.forename: Shengbing
authors.initials: S
authors.lastname: Yang
authors.affiliation: Changhai Hospital, Department of Orthopedics, the Second Military Medical University, Shanghai, People's Republic of China.
authors.forename: Shuogui
authors.initials: S
authors.lastname: Xu
authors.affiliation: Changhai Hospital, Department of Orthopedics, the Second Military Medical University, Shanghai, People's Republic of China.
authors.forename: Panyu
authors.initials: P
authors.lastname: Zhou
authors.affiliation: Key Laboratory for Ultrafine Materials of Ministry of Education, East China University of Science and Technology, Shanghai, People's Republic of China.
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.affiliation: Shanghai Key Laboratory of Orthopedic Implant, Department of Orthopedic Surgery, Shanghai 9th People's Hospital, Shanghai Jiao Tong University School of Medicine China, Shanghai, People's Republic of China.
authors.forename: Honglue
authors.initials: H
authors.lastname: Tan
authors.affiliation: Engineering Research Center for Biomedical Materials of Ministry of Education, East China University of Science and Technology, Shanghai, People's Republic of China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Shanghai Key Laboratory of Orthopedic Implant, Department of Orthopedic Surgery, Shanghai 9th People's Hospital, Shanghai Jiao Tong University School of Medicine China, Shanghai, People's Republic of China.
authors.forename: TingTing
authors.initials: T
authors.lastname: Tang
authors.affiliation: The State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, People's Republic of China ; Key Laboratory for Ultrafine Materials of Ministry of Education, East China University of Science and Technology, Shanghai, People's Republic of China ; Engineering Research Center for Biomedical Materials of Ministry of Education, East China University of Science and Technology, Shanghai, People's Republic of China.
authors.forename: ChangSheng
authors.initials: C
authors.lastname: Liu
mesh_terms: D020155:3-Hydroxybutyric Acid / Q000737:chemistry*; D000818:Animals; D055396:Bone Morphogenetic Protein 2 / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D018786:Bone Substitutes / Q000737:chemistry*; D002208:Caproates / Q000737:chemistry*; D002468:Cell Physiological Phenomena / Q000187:drug effects; D002478:Cells, Cultured; D006801:Humans; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects; D053761:Nanocomposites / Q000737:chemistry* / Q000648:ultrastructure; D011817:Rabbits; D011884:Radius / Q000293:injuries / Q000601:surgery; D011994:Recombinant Proteins / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D012038:Regeneration; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds; D016212:Transforming Growth Factor beta / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D000094122:Polyhydroxybutyrates
chemical_list: D020155:3-Hydroxybutyric Acid; D055396:Bone Morphogenetic Protein 2; D018786:Bone Substitutes; D002208:Caproates; D011994:Recombinant Proteins; D016212:Transforming Growth Factor beta; C115940:poly(3-hydroxybutyrate-co-3-hydroxyhexanoate); D000094122:Polyhydroxybutyrates; C499469:recombinant human bone morphogenetic protein-2
keywords: bone regeneration; mesoporous; rhBMP-2; scaffolds; stem cells