title: Poly(Ethylene glycol)-based backbones with high peptide loading capacities.
abstract: Polymer-peptide conjugates are a promising class of compounds, where polymers can be used to overcome some of the limitations associated with peptides intended for therapeutic and/or diagnostic applications. Linear polymers such as poly(ethylene glycol) can be conjugated through terminal moieties and have therefore limited loading capacities. In this research, functionalised linear poly(ethylene glycol)s are utilised for peptide conjugation, to increase their potential loading capacities. These poly(ethylene glycol) derivatives are conjugated to peptide sequences containing representative side-chain functionalised amino acids, using different conjugation chemistries, including copper-catalysed azide-alkyne cycloaddition, amide coupling and thiol-ene reactions. Conjugation of a sequence containing the RGD motif to poly(allyl glycidyl ether) by the thiol-ene reaction, provided a conjugate which could be used in platelet adhesion studies.
authors.affiliation: Department of Pharmaceutical and Medicinal Chemistry, Centre for Synthesis and Chemical Biology, Royal College of Surgeons in Ireland, 123 St Stephen's Green, Dublin 2, Ireland. aoifemoconnor87@gmail.com.
authors.forename: Aoife
authors.initials: A
authors.lastname: O'Connor
authors.affiliation: Department of Pharmaceutical and Medicinal Chemistry, Centre for Synthesis and Chemical Biology, Royal College of Surgeons in Ireland, 123 St Stephen's Green, Dublin 2, Ireland. marsatjn@yahoo.fr.
authors.forename: Jean-Noel
authors.initials: JN
authors.lastname: Marsat
authors.affiliation: Department of Molecular and Cellular Therapeutics, Royal College of Surgeons in Ireland, 123 St Stephen's Green, Dublin 2, Ireland. annachiaramitrugno@rcsi.ie.
authors.forename: Annachiara
authors.initials: A
authors.lastname: Mitrugno
authors.affiliation: Department of Pharmaceutical and Medicinal Chemistry, Centre for Synthesis and Chemical Biology, Royal College of Surgeons in Ireland, 123 St Stephen's Green, Dublin 2, Ireland. tomflahive@rcsi.ie.
authors.forename: Tom
authors.initials: T
authors.lastname: Flahive
authors.affiliation: Department of Molecular and Cellular Therapeutics, Royal College of Surgeons in Ireland, 123 St Stephen's Green, Dublin 2, Ireland. nmoran@rcsi.ie.
authors.forename: Niamh
authors.initials: N
authors.lastname: Moran
authors.affiliation: UCD School of Veterinary Medicine and UCD Conway Institute, University College Dublin, Belfield, Dublin 4, Ireland. david.brayden@ucd.ie.
authors.forename: David
authors.initials: D
authors.lastname: Brayden
authors.affiliation: Department of Pharmaceutical and Medicinal Chemistry, Centre for Synthesis and Chemical Biology, Royal College of Surgeons in Ireland, 123 St Stephen's Green, Dublin 2, Ireland. mdevocelle@rcsi.ie.
authors.forename: Marc
authors.initials: M
authors.lastname: Devocelle
mesh_terms: D000480:Alkynes / Q000737:chemistry; D000596:Amino Acids / Q000737:chemistry; D001386:Azides / Q000737:chemistry; D001792:Blood Platelets / Q000187:drug effects; D004852:Epoxy Compounds / Q000737:chemistry; D006801:Humans; D010455:Peptides / Q000737:chemistry* / Q000494:pharmacology*; D011092:Polyethylene Glycols / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry; D013438:Sulfhydryl Compounds / Q000737:chemistry
chemical_list: D000480:Alkynes; D000596:Amino Acids; D001386:Azides; D004852:Epoxy Compounds; D010455:Peptides; D011108:Polymers; D013438:Sulfhydryl Compounds; D011092:Polyethylene Glycols; C054361:allyl glycidyl ether