title: Integrin β3 is required in infection and proliferation of classical swine fever virus.
abstract: Classical Swine Fever (CSF) is a highly infectious fatal pig disease, resulting in huge economic loss to the swine industry. Integrins are membrane-bound signal mediators, expressed on a variety of cell surfaces and are known as receptors or co-receptors for many viruses. However, the role of integrin β3 in CSFV infection is unknown. Here, through quantitive PCR, immunofluorescence (IFC) and immunocytohistochemistry (ICC), we revealed that ST (swine testicles epithelial) cells have a prominent advantage in CSFV proliferation as compared to EC (swine umbilical vein endothelial cell), IEC (swine intestinal epithelial cell) and PK (porcine kidney epithelial) cells. Meanwhile, ST cells had remarkably more integrin β3 expression as compared to EC, IEC and PK cells, which was positively correlated with CSFV infection and proliferation. Integrin β3 was up-regulated post CSFV infection in all the four cell lines, while the CSFV proliferation rate was decreased in integrin β3 function-blocked cells. ShRNA1755 dramatically decreased integrin β3, with a deficiency of 96% at the mRNA level and 80% at the protein level. CSFV proliferation was dramatically reduced in integrin β3 constantly-defected cells (ICDC), with the deficiencies of 92.6%, 99% and 81.7% at 24 h, 48 h and 72 h post CSFV infection, respectively. These results demonstrate that integrin β3 is required in CSFV infection and proliferation, which provide a new insight into the mechanism of CSFV infection.
authors.affiliation: College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, China.
authors.forename: Weiwei
authors.initials: W
authors.lastname: Li
authors.affiliation: Jiangsu Key Laboratory of Biological Cancer Therapy, Xuzhou Medical College, Xuzhou, Jiangsu, China.
authors.forename: Gang
authors.initials: G
authors.lastname: Wang
authors.affiliation: College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, China.
authors.forename: Wulong
authors.initials: W
authors.lastname: Liang
authors.affiliation: College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Kang
authors.affiliation: College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, China.
authors.forename: Kangkang
authors.initials: K
authors.lastname: Guo
authors.affiliation: College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, China.
authors.forename: Yanming
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D000818:Animals; D002448:Cell Adhesion; D018929:Cell Culture Techniques; D006691:Classical Swine Fever / Q000821:virology*; D006692:Classical Swine Fever Virus / Q000502:physiology*; D017931:DNA Primers / Q000737:chemistry; D042783:Endothelial Cells / Q000821:virology*; D004847:Epithelial Cells / Q000821:virology; D039661:Integrin beta3 / Q000378:metabolism*; D007422:Intestines / Q000821:virology; D008297:Male; D008856:Microscopy, Fluorescence; D016133:Polymerase Chain Reaction; D034741:RNA, Small Interfering / Q000378:metabolism; D013552:Swine; D013737:Testis / Q000821:virology; D013997:Time Factors; D014357:Trypsin / Q000737:chemistry; D014471:Umbilical Veins / Q000821:virology
chemical_list: D017931:DNA Primers; D039661:Integrin beta3; D034741:RNA, Small Interfering; D014357:Trypsin