title: Preparation of monoclonal antibodies against bovine haptoglobin.
abstract: Female, 8-week-old BALB/c mice were immunized with purified recombinant proteins of the predicted immunodominant region of bovine haptoglobin (pirBoHp). Two monoclonal antibodies (MAbs), named 1B3 and 6D6, were prepared by conventional B lymphocyte hybridoma technique. Titers of ascitic fluid and cell culture supernatant of MAb 1B3 were 1:9.6 × 10(8) and 1:8.2 × 10(4), respectively, and that of MAb 6D6 were 1:4.4 × 10(5) and 1:1.0 × 10(4), respectively. The subtype of MAbs 1B3 and 6D6 was IgG1κ. In Western blot analysis, MAbs 1B3 and 6D6 could recognize the α-chain of native BoHp from plasma of dairy cows. These data indicated that MAbs 1B3 and 6D6 have a potential use for developing diagnostic reagents of BoHp.
authors.affiliation: Department of Veterinary Clinical Medicine, College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University , Daqing, P.R. China .
authors.forename: Caihong
authors.initials: C
authors.lastname: Wang
authors.forename: Cheng
authors.initials: C
authors.lastname: Gu
authors.forename: Donghua
authors.initials: D
authors.lastname: Guo
authors.forename: Jing
authors.initials: J
authors.lastname: Gao
authors.forename: Chunqiu
authors.initials: C
authors.lastname: Li
authors.forename: Na
authors.initials: N
authors.lastname: Liu
authors.forename: Yufei
authors.initials: Y
authors.lastname: Geng
authors.forename: Mingjun
authors.initials: M
authors.lastname: Su
authors.forename: Xinyu
authors.initials: X
authors.lastname: Wang
authors.forename: Dongbo
authors.initials: D
authors.lastname: Sun
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity / Q000276:immunology; D002417:Cattle; D005260:Female; D006242:Haptoglobins / Q000276:immunology*; D006825:Hybridomas / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D011993:Recombinant Fusion Proteins / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D006242:Haptoglobins; D011993:Recombinant Fusion Proteins