title: Ru(III)(EDTA) mediated S-nitrosylation of cysteine by nitrite.
abstract: Reported here is the first example of a ruthenium(iii) complex [Ru(III)(EDTA)(H2O)](-) (EDTA(4-) = ethylenediaminetetraacetate) that mediates S-nitrosylation of cysteine in the presence of nitrite at pH 4.5 (acetate buffer) and results in the formation of [Ru(III)(EDTA)(SNOCy)](-). The kinetics of the reaction was studied by stopped-flow and rapid-scan spectrophotometry as a function of [Cysteine], [NO2(-)] and pH (3.5-8.5). Formation of [Ru(III)(EDTA)(SNOCy)](-), the product of the S-nitrosylation reaction, was identified by ESI-MS experiments. A working mechanism in agreement with the spectroscopic and kinetic data is presented.
authors.affiliation: Chemistry and Biomimetics Group, CSIR-Central Mechanical Engineering Research Institute, MG Avenue, Durgapur-713209, India. dchat57@hotmail.com.
authors.forename: Debabrata
authors.initials: D
authors.lastname: Chatterjee
authors.forename: Namita
authors.initials: N
authors.lastname: Jaiswal
authors.forename: Matthias
authors.initials: M
authors.lastname: Schmeisser
authors.forename: Rudi
authors.initials: R
authors.lastname: van Eldik
mesh_terms: D056831:Coordination Complexes / Q000737:chemistry*; D003545:Cysteine / Q000737:chemistry*; D004492:Edetic Acid / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D009573:Nitrites / Q000737:chemistry*; D012428:Ruthenium / Q000737:chemistry*; D013053:Spectrophotometry
chemical_list: D056831:Coordination Complexes; D009573:Nitrites; D012428:Ruthenium; D004492:Edetic Acid; D003545:Cysteine