title: Dampened neural activity and abolition of epileptic-like activity in cortical slices by active ingredients of spices.
abstract: Active ingredients of spices (AIS) modulate neural response in the peripheral nervous system, mainly through interaction with TRP channel/receptors. The present study explores how different AIS modulate neural response in layer 5 pyramidal neurons of S1 neocortex. The AIS tested are agonists of TRPV1/3, TRPM8 or TRPA1. Our results demonstrate that capsaicin, eugenol, menthol, icilin and cinnamaldehyde, but not AITC dampen the generation of APs in a voltage- and time-dependent manner. This effect was further tested for the TRPM8 ligands in the presence of a TRPM8 blocker (BCTC) and on TRPM8 KO mice. The observable effect was still present. Finally, the influence of the selected AIS was tested on in vitro gabazine-induced seizures. Results coincide with the above observations: except for cinnamaldehyde, the same AIS were able to reduce the number, duration of the AP bursts and increase the concentration of gabazine needed to elicit them. In conclusion, our data suggests that some of these AIS can modulate glutamatergic neurons in the brain through a TRP-independent pathway, regardless of whether the neurons are stimulated intracellularly or by hyperactive microcircuitry.
authors.affiliation: Laboratory of Neural Microcircuitry. Brain and Mind Institute. École Polytechnique Fédérale de Lausanne. Switzerland.
authors.forename: Maurizio
authors.initials: M
authors.lastname: Pezzoli
authors.affiliation: Laboratory of Neural Microcircuitry. Brain and Mind Institute. École Polytechnique Fédérale de Lausanne. Switzerland.
authors.forename: Abdeladim
authors.initials: A
authors.lastname: Elhamdani
authors.affiliation: 1] Laboratory of Neural Microcircuitry. Brain and Mind Institute. École Polytechnique Fédérale de Lausanne. Switzerland [2] Laboratory of Perception Physiology. Nestlé Research Center. Lausanne, Switzerland.
authors.forename: Susana
authors.initials: S
authors.lastname: Camacho
authors.affiliation: Laboratory of Neural Microcircuitry. Brain and Mind Institute. École Polytechnique Fédérale de Lausanne. Switzerland.
authors.forename: Julie
authors.initials: J
authors.lastname: Meystre
authors.affiliation: Laboratory of Perception Physiology. Nestlé Research Center. Lausanne, Switzerland.
authors.forename: Stephanie Michlig
authors.initials: SM
authors.lastname: González
authors.affiliation: 1] Laboratory of Perception Physiology. Nestlé Research Center. Lausanne, Switzerland [2] Organization for Interdisciplinary Research Projects, The University of Tokyo, Tokyo, Japan.
authors.forename: Johannes
authors.initials: J
authors.lastname: le Coutre
authors.affiliation: Laboratory of Neural Microcircuitry. Brain and Mind Institute. École Polytechnique Fédérale de Lausanne. Switzerland.
authors.forename: Henry
authors.initials: H
authors.lastname: Markram
mesh_terms: D000200:Action Potentials / Q000187:drug effects*; D000818:Animals; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000187:drug effects / Q000378:metabolism*; D004827:Epilepsy / Q000188:drug therapy / Q000378:metabolism*; D008610:Menthol / Q000494:pharmacology; D051379:Mice; D009474:Neurons / Q000187:drug effects / Q000378:metabolism*; D010936:Plant Extracts / Q000494:pharmacology*; D017365:Spices; D050053:TRPM Cation Channels / Q000378:metabolism; D050051:Transient Receptor Potential Channels / Q000378:metabolism
chemical_list: D010936:Plant Extracts; D050053:TRPM Cation Channels; C492297:TRPM8 protein, mouse; D050051:Transient Receptor Potential Channels; D008610:Menthol