title: Color test for selective detection of secondary amines on resin and in solution.
abstract: Resins for solid-phase synthesis give orange to red-brown resin beads selectively when secondary amines are present on the resin when treated with a solution of acetaldehyde and an Fmoc-amino acid in NMP. The method shows good specificity and gives colorless beads when exposed to a variety of other functional groups. Furthermore, the acetaldehyde/Fmoc amino acid method can be used as a selective colorimetric test for secondary amines in solution.
authors.affiliation: The National Veterinary Institute, Technical University of Denmark . Department of Immunology and Vaccinology, Bülowsvej 27, DK-1870, Frederiksberg, Denmark.
authors.forename: Ulrik
authors.initials: U
authors.lastname: Boas
authors.forename: Sahar
authors.initials: S
authors.lastname: Mirsharghi
mesh_terms: D000079:Acetaldehyde / Q000737:chemistry; D000588:Amines / Q000032:analysis* / Q000737:chemistry; D000596:Amino Acids / Q000737:chemistry; D003124:Colorimetry / Q000379:methods*; D005449:Fluorenes / Q000737:chemistry; D015394:Molecular Structure; D012117:Resins, Synthetic / Q000737:chemistry*; D060327:Solid-Phase Synthesis Techniques; D012996:Solutions
chemical_list: D000588:Amines; D000596:Amino Acids; D005449:Fluorenes; C016456:N(alpha)-fluorenylmethyloxycarbonylamino acids; D012117:Resins, Synthetic; D012996:Solutions; D000079:Acetaldehyde