title: Regenerative endodontic treatment of an immature tooth with a necrotic pulp and apical periodontitis using platelet-rich plasma (PRP) and mineral trioxide aggregate (MTA): a case report.
abstract: OBJECTIVE
  To report the successful clinical and radiographic outcome of a regenerative endodontic treatment.
  
  
  CONCLUSIONS
  A 16-year-old male patient presented with a discoloured, maxillary left lateral incisor with a necrotic pulp. Radiographic examination revealed an incompletely developed root with an open apex. Under local anaesthesia and rubber dam isolation, an access cavity was prepared and the necrotic pulpal remnants were removed. The canal was disinfected without mechanical instrumentation with 5.25% NaOCl solution and dried with sterile paper points. A triple antibiotic (metronidazole, ciprofloxacin and minocycline) mixed with distilled water was packed in the canal and left for 28 days. Ten millimetres of whole blood was drawn by venipuncture from the patients antecubital vein for preparation of platelet-rich plasma (PRP). After removal of the antibiotic mixture, the PRP was injected into the canal space up to the cementoenamel junction level. Three millimetres of white MTA was placed directly over the PRP clot. Two days later, the tooth was restored with permanent filling materials. The patient was recalled for 3, 6, 12, 24 and 36 months clinical/radiographic follow-up. A 3-year follow-up radiograph revealed resolution of the periapical lesion, increased thickening of the root walls, further root development and continued apical closure of the root apex. The tooth was not responsive to cold tests; however, sensitivity tests with an electric pulp tester (EPT) elicited a delayed positive response.
  
  
  CONCLUSIONS
  Regeneration is a viable treatment modality that allows continued root development of immature teeth with open apices and necrotic pulps. Platelet-rich plasma appears to be a suitable scaffold for regeneration of vital tissues in teeth with a necrotic pulps and an associated periapical lesion. Regenerative endodontic procedures may offer an effective treatment option to save teeth with compromised structural integrity.
authors.affiliation: Department of Conservative Dentistry and Endodontics, Himachal Dental College and Hospital, Sundernagar, India.
authors.forename: G S
authors.initials: GS
authors.lastname: Sachdeva
authors.affiliation: Department of Conservative Dentistry and Endodontics, Himachal Dental College and Hospital, Sundernagar, India.
authors.forename: L T
authors.initials: LT
authors.lastname: Sachdeva
authors.affiliation: Department of Conservative Dentistry and Endodontics, Himachal Dental College and Hospital, Sundernagar, India.
authors.forename: M
authors.initials: M
authors.lastname: Goel
authors.affiliation: Department of Conservative Dentistry and Endodontics, Himachal Dental College and Hospital, Sundernagar, India.
authors.forename: S
authors.initials: S
authors.lastname: Bala
mesh_terms: D000293:Adolescent; D017607:Aluminum Compounds; D000900:Anti-Bacterial Agents / Q000627:therapeutic use; D017610:Calcium Compounds; D003790:Dental Pulp Necrosis / Q000628:therapy*; D004338:Drug Combinations; D006801:Humans; D007180:Incisor / Q000503:physiopathology; D008297:Male; D010087:Oxides; D010485:Periapical Periodontitis / Q000628:therapy*; D053657:Platelet-Rich Plasma; D012038:Regeneration; D012390:Root Canal Therapy / Q000379:methods*; D017640:Silicates; D014092:Tooth Root / Q000503:physiopathology
chemical_list: D017607:Aluminum Compounds; D000900:Anti-Bacterial Agents; D017610:Calcium Compounds; D004338:Drug Combinations; D010087:Oxides; D017640:Silicates; C086631:mineral trioxide aggregate
keywords: incisor; mineral trioxide aggregate (MTA); platelet-rich plasma (PRP); regeneration; revascularisation