title: Up-regulation of Glis2 involves in neuronal apoptosis after intracerebral hemorrhage in adult rats.
abstract: The novel Krüppel-like zinc finger protein Gli-similar 2 (Glis2), one member of the transcription factors, is involved in controlling the flow of genetic information and the modulation of diverse cellular activities. Accumulating evidence has demonstrated its important roles in adult development and several diseases. However, information regarding the regulation and possible function of Glis2 in the central nervous system is still limited. In this study, we explored the roles of Glis2 during the pathophysiological process of intracerebral hemorrhage (ICH). An ICH rat model was established and assessed by behavioral tests. Expression of Glis2 was significantly up-regulated in brain areas surrounding the hematoma following ICH. Immunofluorescence showed that Glis2 was strikingly increased in neurons, but not astrocytes or microglia. Up-regulation of Glis2 was found to be accompanied by the increased expression of active caspase-3 and Bax and decreased expression of Bcl-2 in vivo and vitro studies. Moreover, knocking down Glis2 by RNA-interference in PC12 cells reduced active caspase-3 and Bax expression while increased Bcl-2. Collectively, we speculated that Glis2 might exert pro-apoptotic function in neurons following ICH.
authors.affiliation: Department of Neurology, Affiliated Hospital of Nantong University, Nantong, 226001, Jiangsu Province, People's Republic of China.
authors.forename: Kaifu
authors.initials: K
authors.lastname: Ke
authors.affiliation: Department of Neurology, Affiliated Hospital of Nantong University, Nantong, 226001, Jiangsu Province, People's Republic of China.|Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Nantong University, Nantong, 226001, Jiangsu Province, People's Republic of China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Song
authors.affiliation: Department of Neurology, Affiliated Hospital of Nantong University, Nantong, 226001, Jiangsu Province, People's Republic of China.|Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Nantong University, Nantong, 226001, Jiangsu Province, People's Republic of China.
authors.forename: Jiabing
authors.initials: J
authors.lastname: Shen
authors.affiliation: Department of Neurology, Affiliated Hospital of Nantong University, Nantong, 226001, Jiangsu Province, People's Republic of China.|Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Nantong University, Nantong, 226001, Jiangsu Province, People's Republic of China.
authors.forename: Mu
authors.initials: M
authors.lastname: Niu
authors.affiliation: Department of Neurology, Affiliated Hospital of Nantong University, Nantong, 226001, Jiangsu Province, People's Republic of China.
authors.forename: Haiyan
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Department of Neurology, Affiliated Hospital of Nantong University, Nantong, 226001, Jiangsu Province, People's Republic of China.
authors.forename: Daming
authors.initials: D
authors.lastname: Yuan
authors.affiliation: Department of Neurology, Affiliated Hospital of Nantong University, Nantong, 226001, Jiangsu Province, People's Republic of China.
authors.forename: Haidan
authors.initials: H
authors.lastname: Ni
authors.affiliation: Department of Neurology, Affiliated Hospital of Nantong University, Nantong, 226001, Jiangsu Province, People's Republic of China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Neurology, Affiliated Hospital of Nantong University, Nantong, 226001, Jiangsu Province, People's Republic of China.
authors.forename: Xiaorong
authors.initials: X
authors.lastname: Liu
authors.affiliation: Department of Neurology, Affiliated Hospital of Nantong University, Nantong, 226001, Jiangsu Province, People's Republic of China.
authors.forename: Aihua
authors.initials: A
authors.lastname: Dai
authors.affiliation: Department of Neurology, Affiliated Hospital of Nantong University, Nantong, 226001, Jiangsu Province, People's Republic of China. cmhongnt@sina.com.
authors.forename: Maohong
authors.initials: M
authors.lastname: Cao
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D002543:Cerebral Hemorrhage / Q000378:metabolism* / Q000473:pathology; D051741:Kruppel-Like Transcription Factors / Q000096:biosynthesis*; D008297:Male; D009474:Neurons / Q000378:metabolism* / Q000473:pathology; D016716:PC12 Cells; D051381:Rats; D017207:Rats, Sprague-Dawley; D015854:Up-Regulation / Q000502:physiology*
chemical_list: C447919:GLIS2 protein, human; D051741:Kruppel-Like Transcription Factors