title: Expression and localization of type II diacylglycerol kinase isozymes δ and η in the developing mouse brain.
abstract: The functions of type II diacylglycerol kinase (DGK) δ and -η in the brain are still unclear. As a first step, we investigated the spatial and temporal expression of DGKδ and -η in the brains of mice. DGKδ2, but not DGKδ1, was highly expressed in layers II-VI of the cerebral cortex; CA-CA3 regions and dentate gyrus of hippocampus; mitral cell, glomerular and granule cell layers of the olfactory bulb; and the granule cell layer in the cerebellum in 1- to 32-week-old mice. DGKδ2 was expressed just after birth, and its expression levels dramatically increased from weeks 1 to 4. A substantial amount of DGKη (η1/η2) was detected in layers II-VI of the cerebral cortex, CA1 and CA2 regions and dentate gyrus of the hippocampus, mitral cell and glomerular layers of the olfactory bulb, and Purkinje cells in the cerebellum of 1- to 32-week-old mice. DGKη2 expression reached maximum levels at P5 and decreased by 4 weeks, whereas DGKη1 increased over the same time frame. These results indicate that the expression patterns of DGK isozymes differ from each other and also from other isozymes, and this suggests that DGKδ and -η play distinct and specific roles in the brain.
authors.affiliation: Department of Chemistry, Graduate School of Science, Chiba University, Chiba, Japan. (TU, HS, TS, FS)
authors.forename: Takako
authors.initials: T
authors.lastname: Usuki
authors.affiliation: Department of Chemistry, Graduate School of Science, Chiba University, Chiba, Japan. (TU, HS, TS, FS)
authors.forename: Hiromichi
authors.initials: H
authors.lastname: Sakai
authors.affiliation: Department of Chemistry, Graduate School of Science, Chiba University, Chiba, Japan. (TU, HS, TS, FS)
authors.forename: Takao
authors.initials: T
authors.lastname: Shionoya
authors.affiliation: Department of Nanobiology, Graduate School of Advanced Integration Science, Chiba University, Chiba Japan. (NS)
authors.forename: Naruki
authors.initials: N
authors.lastname: Sato
authors.affiliation: Department of Chemistry, Graduate School of Science, Chiba University, Chiba, Japan. (TU, HS, TS, FS)
authors.forename: Fumio
authors.initials: F
authors.lastname: Sakane
mesh_terms: D000818:Animals; D001921:Brain / Q000201:enzymology* / Q000254:growth & development*; D019852:Diacylglycerol Kinase / Q000235:genetics* / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism; D051379:Mice; D021381:Protein Transport; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D062211:Spatio-Temporal Analysis
chemical_list: D007527:Isoenzymes; D012333:RNA, Messenger; D019852:Diacylglycerol Kinase
keywords: Purkinje cell; age-dependent expression; bipolar disorder; central nerves system; cerebral cortex; diacylglycerol kinase; epileptic seizure; hippocampus; in situ hybridization; olfactory bulb