title: Negligible effects of tryptophan on the aflatoxin adsorption of sodium bentonite.
abstract: The main objective of this study was to determine if the competitive adsorption of tryptophan (Trp) and aflatoxin B₁ (AFB₁) could potentially affect the ability of a sodium bentonite (NaB) to prevent aflatoxicosis in monogastric animals. The adsorption of Trp and AFB₁ on this adsorbent is fast and could be operating on the same time-scale making competition feasible. In vitro competitive adsorption experiments under simulated gastrointestinal conditions were performed. A high affinity of the clay for Trp and NaB was observed. The effect of an excess of KCl to mimic the ionic strength of the physiological conditions were also investigated. A six-times decrease in the Trp surface excess at saturation was observed. A similar behaviour was previously found for AFB₁ adsorption. Taking into account the amount of Trp adsorbed by the clay and the usual adsorbent supplementation level in diets, a decrease in Trp bioavailability is not expected to occur. Tryptophan adsorption isotherms on NaB were 'S'-shaped and were adjusted by the Frumkin-Fowler-Guggenheim model. The reversibility of the adsorption processes was investigated in order to check a potential decrease in the ability of NaB to protect birds against chronic aflatoxicoses. Adsorption processes were completely reversible for Trp, while almost irreversible for AFB₁. In spite of the high affinity of the NaB for Trp, probably due to the reversible character of Trp adsorption, no changes in the AFB₁ adsorption isotherm were observed when an excess of the amino acid was added to the adsorption medium. As a consequence of the preferential and irreversible AFB₁ adsorption and the reversible weak binding of Trp to the NaB, no changes in the aflatoxin sorption ability of the clay are expected to occur in the gastrointestinal tract of birds.
authors.affiliation: a Departamento de Microbiología e Inmunología , Universidad Nacional de Río Cuarto , Pergamino , Argentina.
authors.forename: A P
authors.initials: AP
authors.lastname: Magnoli
authors.forename: P
authors.initials: P
authors.lastname: Copia
authors.forename: M P
authors.initials: MP
authors.lastname: Monge
authors.forename: C E
authors.initials: CE
authors.lastname: Magnoli
authors.forename: A M
authors.initials: AM
authors.lastname: Dalcero
authors.forename: S M
authors.initials: SM
authors.lastname: Chiacchiera
mesh_terms: D000327:Adsorption; D016604:Aflatoxin B1 / Q000037:antagonists & inhibitors / Q000737:chemistry* / Q000378:metabolism; D000821:Animal Feed; D000818:Animals; D001118:Argentina; D001546:Bentonite / Q000737:chemistry* / Q000378:metabolism; D001667:Binding, Competitive; D002274:Carcinogens, Environmental / Q000737:chemistry* / Q000378:metabolism; D002614:Chelating Agents / Q000737:chemistry* / Q000378:metabolism; D005240:Feasibility Studies; D005503:Food Additives / Q000737:chemistry / Q000378:metabolism; D005506:Food Contamination / Q000517:prevention & control; D005766:Gastrointestinal Contents; D007700:Kinetics; D008956:Models, Chemical; D009994:Osmolar Concentration; D011200:Poultry; D014364:Tryptophan / Q000737:chemistry* / Q000378:metabolism
chemical_list: D002274:Carcinogens, Environmental; D002614:Chelating Agents; D005503:Food Additives; D001546:Bentonite; D014364:Tryptophan; D016604:Aflatoxin B1
keywords: aflatoxin B1; competitive adsorption; sodium bentonite; tryptophan