title: The Illumina-solexa sequencing protocol for bacterial genomes.
abstract: Based on reversible dye-terminators technology, the Illumina-solexa sequencing platform enables rapid sequencing-by-synthesis (SBS) of large DNA stretches spanning entire genomes, with the latest instruments capable of producing hundreds of gigabases of data in a single sequencing run. Illumina's NGS instruments powerfully combine the flexibility of single reads with short- and long-insert paired-end reads, and enable a wide range of DNA sequencing applications. Here, we describe the paired-end library preparation with an average insert size of 470 bp, 2 kbp, and 6 kbp, together with the DNA cluster generation and sequencing procedure of E. coli O104:H4 genome on Illumina Hiseq 2000 platform.
authors.affiliation: BGI, Beishan Road, Shenzen, 518083, China.
authors.forename: Zhenfei
authors.initials: Z
authors.lastname: Hu
authors.forename: Lei
authors.initials: L
authors.lastname: Cheng
authors.forename: Hai
authors.initials: H
authors.lastname: Wang
mesh_terms: D020451:Contig Mapping / Q000379:methods*; D015342:DNA Probes / Q000138:chemical synthesis / Q000737:chemistry; D004269:DNA, Bacterial; D004587:Electrophoresis, Agar Gel; D004926:Escherichia coli / Q000235:genetics*; D016680:Genome, Bacterial; D015698:Genomic Library; D059014:High-Throughput Nucleotide Sequencing / Q000295:instrumentation / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods; D017422:Sequence Analysis, DNA / Q000706:statistics & numerical data
chemical_list: D015342:DNA Probes; D004269:DNA, Bacterial