title: Transcriptome analysis of epithelioma papulosum cyprini cells after SVCV infection.
abstract: BACKGROUND
  Spring viraemia of carp virus (SVCV) has been identified as the causative agent of spring viraemia of carp (SVC) and it has caused significant losses in the cultured common carp (Cyprinus carpio) industry. The molecular mechanisms that underlie the pathogenesis of the disease remain poorly understood. In this study, deep RNA sequencing was used to analyse the transcriptome and gene expression profile of EPC cells at progressive times after SVCV infection. This study addressed the complexity of virus-cell interactions and added knowledge that may help to understand SVCV.
  
  
  RESULTS
  A total of 33,849,764 clean data from 36,000,000 sequence reads, with a mean read length 100 bp, were obtained. These raw data were assembled into 88,772 contigs. Of these contigs, 19,642 and 25,966 had significant hits to the NR and Uniprot databases where they matched 17,642 and 13,351 unique protein accessions, respectively. At 24 h post SVCV infection (1.0 MOI), a total of 623 genes were differentially expressed in EPC cells compared to non-infected cells, including 288 up-regulated genes and 335 down-regulated genes. These regulated genes were primarily involved in pathways of apoptosis, oxidative stress and the interferon system, all of which may be involved in viral pathogenesis. In addition, 8 differentially expressed genes (DEGs) were validated by quantitative PCR.
  
  
  CONCLUSIONS
  Our findings demonstrate previously unrecognised changes in gene transcription that are associated with SVCV infection in vitro, and many potential cascades identified in the study clearly warrant further experimental investigation. Our data provide new clues to the mechanism of viral susceptibility in EPC cells.
authors.forename: Junfa
authors.initials: J
authors.lastname: Yuan
authors.forename: Yi
authors.initials: Y
authors.lastname: Yang
authors.forename: Huihui
authors.initials: H
authors.lastname: Nie
authors.forename: Lijuan
authors.initials: L
authors.lastname: Li
authors.forename: Wangang
authors.initials: W
authors.lastname: Gu
authors.forename: Li
authors.initials: L
authors.lastname: Lin
authors.forename: Min
authors.initials: M
authors.lastname: Zou
authors.forename: Xueqin
authors.initials: X
authors.lastname: Liu
authors.forename: Min
authors.initials: M
authors.lastname: Wang
authors.affiliation: Department of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, Wuhan 430070, People's Republic of China. guzemao@mail.hzau.edu.cn.
authors.forename: Zemao
authors.initials: Z
authors.lastname: Gu
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000235:genetics; D002347:Carps / Q000821:virology*; D045744:Cell Line, Tumor; D018450:Disease Progression; D005393:Fish Diseases / Q000235:genetics*; D020869:Gene Expression Profiling; D059014:High-Throughput Nucleotide Sequencing; D007372:Interferons / Q000378:metabolism; D007700:Kinetics; D058977:Molecular Sequence Annotation; D018384:Oxidative Stress / Q000235:genetics; D012209:Rhabdoviridae / Q000502:physiology*; D018353:Rhabdoviridae Infections / Q000235:genetics* / Q000378:metabolism / Q000473:pathology
chemical_list: D007372:Interferons