title: Developmental validation of the AGCU 21+1 STR kit: a novel multiplex assay for forensic application.
abstract: In this study, we describe the developmental validation assay performed on a novel designed STR multiplex system, AGCU 21+1 STR kit. This kit contains a sex-determining locus amelogenin and 21 noncombined DNA index system STR loci, that are, D6S474, D12ATA63, D22S1045, D10S1248, D1S1677, D11S4463, D1S1627, D3S4529, D2S441, D6S1017, D4S2408, D19S433, D17S1301, D1GATA113, D18S853, D20S482, D14S1434, D9S1122, D2S1776, D10S1435, and D5S2500. The 21+1 kit was validated by a series of tests including optimized PCR conditions, sensitivity, precision and accuracy, stutter ratio, DNA mixture, inhibitors, and species specificity according to the revised validation guidelines issued by the Scientific Working Group on DNA Analysis Methods (SWGDAM). Our results in this study show that the kit is a useful tool for forensic application.
authors.affiliation: Research Center of Stomatology, Stomatology Hospital, Xi'an Jiaotong University, Xi'an, China.
authors.forename: Bo-Feng
authors.initials: BF
authors.lastname: Zhu
authors.forename: Yu-Dang
authors.initials: YD
authors.lastname: Zhang
authors.forename: Chun-Mei
authors.initials: CM
authors.lastname: Shen
authors.forename: Wei-An
authors.initials: WA
authors.lastname: Du
authors.forename: Wen-Juan
authors.initials: WJ
authors.lastname: Liu
authors.forename: Hao-Tian
authors.initials: HT
authors.lastname: Meng
authors.forename: Hong-Dan
authors.initials: HD
authors.lastname: Wang
authors.forename: Guang
authors.initials: G
authors.lastname: Yang
authors.forename: Rui
authors.initials: R
authors.lastname: Jin
authors.forename: Chun-Hua
authors.initials: CH
authors.lastname: Yang
authors.forename: Jiang-Wei
authors.initials: JW
authors.lastname: Yan
authors.forename: Xiao-Hua
authors.initials: XH
authors.lastname: Bie
mesh_terms: D000818:Animals; D017931:DNA Primers; D044707:Forensic Sciences / Q000379:methods; D006801:Humans; D008274:Magnesium; D018895:Microsatellite Repeats; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods*; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity; D012732:Sex Determination Analysis; D013045:Species Specificity; D019914:Taq Polymerase; D013696:Temperature
chemical_list: D017931:DNA Primers; D011933:Reagent Kits, Diagnostic; D019914:Taq Polymerase; D008274:Magnesium
keywords: AGCU 21+1 STR kit; DNA genotyping; Forensic validation; Short tandem repeat