title: Isolation and expression of enolase gene in Fusarium oxysporum f. sp. lycopersici.
abstract: Fusarium oxysporum f. sp. lycopersici is a fungus responsible for the tomato disease known as fusariosis. Enolase, which is the enzyme that catalyzes the reaction of 2-phosphoglycerate to phosphoenolpyruvate, is present during glycolysis. Enolase genes have been isolated from bacteria and fungi, among other organisms. In this research, a large portion of the enolase, eno, gene sequence was isolated from F. oxysporum and compared with those of other microorganisms, revealing a similarity of 51-69 %. We analyzed the copy number of the eno gene and determined that only a single copy is present in F. oxysporum, as in several fungi, such as Candida albicans and Aspergillus oryzae. We also detected the expression of the eno gene by reverse transcription-polymerase chain reaction during in vitro growth under two growth conditions where glucose was used as the carbon source, and we observed the same eno gene expression levels under both growth conditions.
authors.affiliation: Unidad Profesional Interdisciplinaria de Ingeniería Campus Guanajuato del Instituto Politécnico Nacional (UPIIG-IPN), Avenida Mineral de Valenciana No. 200, Fraccionamiento Industrial Puerto Interior, 36275, Silao de la Victoria, Guanajuato, Mexico, kmaciass@ipn.mx.
authors.forename: Karla Lizbeth
authors.initials: KL
authors.lastname: Macías-Sánchez
authors.forename: Jesús
authors.initials: J
authors.lastname: García-Soto
authors.forename: M Isabel G
authors.initials: MI
authors.lastname: Roncero
authors.forename: Wendy
authors.initials: W
authors.lastname: Hernández-Monjaraz
authors.forename: César
authors.initials: C
authors.lastname: Caudillo-Pérez
authors.forename: Ma Guadalupe
authors.initials: MG
authors.lastname: Martínez-Cadena
mesh_terms: D000595:Amino Acid Sequence; D005670:Fusarium / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D015870:Gene Expression; D008969:Molecular Sequence Data; D010751:Phosphopyruvate Hydratase / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification*
chemical_list: D010751:Phosphopyruvate Hydratase