A novel method HPLC-DAD analysis of the Contentsof Moutan Cortexand Paeoniae Radix Alba with similar constituents-monoterpene glycosides in Guizhi Fuling Wan.
A variety of traditional Chinese medical formulations contain two or more herbs from the same genus or family. Although these herbs may have a similar appearance and constituents, they usually have different pharmacodynamic actions. A series of qualitative and quantitative analysis methods are developed to determine one or more compounds for quality control of medicine. As far as we know, no method has been found to determine the real ratio of the two herbs along with the prescription. In this study, we used HPLC-DAD as a way to determine the content of Moutan cortex (M) and Paeoniae radix alba (P) in GuizhiFuling Wan (GZFLW). An effective, accurate and reliable HPLC-DAD method was developed for detecting the content of M and P in GZFLW through the analysis of four monoterpeneglycosides, namely, galloylpaeoniflorin (1), paeoniflorin (2), mudanpioside C (3) and benzoylpaeoniflorin (4). Due to the different UV characteristics of the compounds, the detection wavelength was 270 nm for 1 and 2, while 3 and 4 were monitored at 254 nm and 230 nm, respectively. Four equations were put forward to describe the relationship between content of M as well as P and the four monoterpene glycosides in GZFLW. After validation, all the accuracies of the M and P contents in GZFLW were within 10%. The result showed that the method could be successfully applied to analyze the contents of M and P in GZFLW. Moreover, our method may be more widely used to control the quality of proprietary Chinese medicines, especially for those containing the same genus or family herbs, in industrial GMP production.
School of Traditional Chinese Materia Medica & Key Laboratory of Structure-Based Drug Design and Discovery of Ministry of Education, Shenyang Pharmaceutical University, Shenyang 110016, China. wangshuyun0426@163.com.
Shuyun
S
Wang
School of Traditional Chinese Materia Medica & Key Laboratory of Structure-Based Drug Design and Discovery of Ministry of Education, Shenyang Pharmaceutical University, Shenyang 110016, China. huangjian_703@163.com.
Jian
J
Huang
School of Traditional Chinese Materia Medica & Key Laboratory of Structure-Based Drug Design and Discovery of Ministry of Education, Shenyang Pharmaceutical University, Shenyang 110016, China. maohuijuan123@163.com.
Huijuan
H
Mao
School of Traditional Chinese Materia Medica & Key Laboratory of Structure-Based Drug Design and Discovery of Ministry of Education, Shenyang Pharmaceutical University, Shenyang 110016, China. wangyuliang123@163.com.
Yuliang
Y
Wang
School of Pharmacy, Xinjiang Medical University, Urumqi 830054, China. renakasimu@vip.sina.com.
Rena
R
Kasimu
Jiangsu Kanion Pharmaceut Co Ltd, Lianyungang 222001, China. xmb@kanion.com.
Wei
W
Xiao
School of Traditional Chinese Materia Medica & Key Laboratory of Structure-Based Drug Design and Discovery of Ministry of Education, Shenyang Pharmaceutical University, Shenyang 110016, China. 15999290001@163.com.
Jinhui
J
Wang
D019086:Bridged Bicyclo Compounds, Heterocyclic / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D005960:Glucosides / Q000737:chemistry; D006027:Glycosides / Q000737:chemistry*; D008516:Medicine, Chinese Traditional / Q000379:methods; D039821:Monoterpenes / Q000737:chemistry*; D029596:Paeonia / Q000737:chemistry*; D018517:Plant Roots / Q000737:chemistry*
D019086:Bridged Bicyclo Compounds, Heterocyclic; D004365:Drugs, Chinese Herbal; D005960:Glucosides; D006027:Glycosides; D039821:Monoterpenes; C119233:galloylpaeoniflorin; C525079:guizhi-fuling; C038881:moutan cortex; C015423:peoniflorin; C047591:benzoylpaeoniflorin