A high-density genetic map identifies a novel major QTL for boron efficiency in oilseed rape (Brassica napus L.).
Low boron (B) seriously limits the growth of oilseed rape (Brassica napus L.), a high B demand species that is sensitive to low B conditions. Significant genotypic variations in response to B deficiency have been observed among B. napus cultivars. To reveal the genetic basis for B efficiency in B. napus, quantitative trait loci (QTLs) for the plant growth traits, B uptake traits and the B efficiency coefficient (BEC) were analyzed using a doubled haploid (DH) population derived from a cross between a B-efficient parent, Qingyou 10, and a B-inefficient parent, Westar 10. A high-density genetic map was constructed based on single nucleotide polymorphisms (SNPs) assayed using Brassica 60 K Infinium BeadChip Array, simple sequence repeats (SSRs) and amplified fragment length polymorphisms (AFLPs). The linkage map covered a total length of 2139.5 cM, with 19 linkage groups (LGs) and an average distance of 1.6 cM between adjacent markers. Based on hydroponic evaluation of six B efficiency traits measured in three separate repeated trials, a total of 52 QTLs were identified, accounting for 6.14-46.27% of the phenotypic variation. A major QTL for BEC, qBEC-A3a, was co-located on A3 with other QTLs for plant growth and B uptake traits under low B stress. Using a subset of substitution lines, qBEC-A3a was validated and narrowed down to the interval between CNU384 and BnGMS436. The results of this study provide a novel major locus located on A3 for B efficiency in B. napus that will be suitable for fine mapping and marker-assisted selection breeding for B efficiency in B. napus.
National Key Laboratory of Crop Genetic Improvement, and Microelement Research Centre, Huazhong Agricultural University, Wuhan, China.
Didi
D
Zhang
National Key Laboratory of Crop Genetic Improvement, and Microelement Research Centre, Huazhong Agricultural University, Wuhan, China.
Yingpeng
Y
Hua
National Key Laboratory of Crop Genetic Improvement, and Microelement Research Centre, Huazhong Agricultural University, Wuhan, China.
Xiaohua
X
Wang
National Key Laboratory of Crop Genetic Improvement, and Microelement Research Centre, Huazhong Agricultural University, Wuhan, China.
Hua
H
Zhao
National Key Laboratory of Crop Genetic Improvement, and Microelement Research Centre, Huazhong Agricultural University, Wuhan, China.
Lei
L
Shi
National Key Laboratory of Crop Genetic Improvement, and Microelement Research Centre, Huazhong Agricultural University, Wuhan, China.
Fangsen
F
Xu
D001895:Boron / Q000378:metabolism* / Q000493:pharmacokinetics; D029688:Brassica napus / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D008040:Genetic Linkage; D005828:Genetics, Population; D006238:Haploidy; D018527:Hydroponics; D018895:Microsatellite Repeats; D010641:Phenotype; D020641:Polymorphism, Single Nucleotide; D040641:Quantitative Trait Loci
D001895:Boron