L-carnitine effectively improves the metabolism and quality of platelet concentrates during storage.
Human platelets undergo structural and biochemical alternations during storage which are collectively called platelet storage lesion (PSL). PSL is characterized as metabolic and functionally changes. It causes decrease in platelet recovery and survival. Here, we evaluated the effect of L-carnitine (LC) on the metabolism, function, and mitochondrial metabolic activity of platelet during storage. Platelet-rich plasma was used to prepare platelet concentrate (PC) in Iranian Blood Transfusion Organization. For this purpose, ten PC bags from healthy donors were stored at 22 °C with gentle agitation in the presence or absence of LC. The effects of LC (15 mM) on the platelet quality were assessed by analyzing the levels of glucose, lactate, ATP, and lactate dehydrogenase (LDH) activity. Platelet aggregations induced by arachidonate and ristocetin were analyzed by aggregometer. Platelet mitochondrial melablolic activity was measured by tetrazolium salt 3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyl tetrazolium bromide (MTT) assay; platelet count and mean platelet volume were also determined by a hematology analyzer during 5 days of PC storage. The results indicated that LC could significantly decrease lactate concentration and glucose consumption accompanied with the increased oxygen consumption in stored PC. LDH activity also less significantly increased in LC-treated PC on days 2 and 5 of storage. Platelet aggregation in response to the ristocetin and arachidonate was significantly higher in LC-treated PC than that in untreated PC on day 5 of storage. Finally, platelet mitochondrial metabolic activity less significantly decreased in LC-treated PC compared to the control group on days 2 and 5 of storage. It seems that LC would be a good additive to reduce PSL and improve the platelet metabolism and quality of the stored PC for platelet transfusion therapy.
Department of Clinical Biochemistry, Faculty of Medical Sciences, Tarbiat Modares University, P.O. Box: 14115-331, 14117-13116, Tehran, Iran.
Mohammad Reza
MR
Deyhim
Seyed Alireza
SA
Mesbah-Namin
Fatemeh
F
Yari
Mohammad
M
Taghikhani
Naser
N
Amirizadeh
D016718:Arachidonic Acid / Q000494:pharmacology; D001792:Blood Platelets / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D001793:Blood Preservation / Q000379:methods* / Q000592:standards; D002331:Carnitine / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D006801:Humans; D019344:Lactic Acid / Q000378:metabolism; D010865:Pilot Projects; D010974:Platelet Aggregation / Q000187:drug effects; D010976:Platelet Count; D053657:Platelet-Rich Plasma / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D011786:Quality Control; D012310:Ristocetin / Q000494:pharmacology
D012310:Ristocetin; D016718:Arachidonic Acid; D019344:Lactic Acid; D002331:Carnitine