Porcine SLITRK1: Molecular cloning and characterization.
The membrane protein SLITRK1 functions as a developmentally regulated stimulator of neurite outgrowth and variants in this gene have been implicated in Tourette syndrome. In the current study we have cloned and characterized the porcine SLITRK1 gene. The genomic organization of SLITRK1 lacks introns, as does its human and mouse counterparts. RT-PCR cloning revealed two SLITRK1 transcripts: a full-length mRNA and a transcript variant that results in a truncated protein. The encoded SLITRK1 protein, consisting of 695 amino acids, displays a very high homology to human SLITRK1 (99%). The porcine SLITRK1 gene is expressed exclusively in brain tissues.
Department of Molecular Biology and Genetics, Aarhus University, Blichers Alle 20, DK-8830 Tjele, Denmark.
Knud
K
Larsen
Department of Molecular Biology and Genetics, Aarhus University, Blichers Alle 20, DK-8830 Tjele, Denmark.
Jamal
J
Momeni
Department of Molecular Biology and Genetics, Aarhus University, Blichers Alle 20, DK-8830 Tjele, Denmark.
Leila
L
Farajzadeh
Department of Molecular Biology and Genetics, Aarhus University, Blichers Alle 20, DK-8830 Tjele, Denmark.
Christian
C
Bendixen
ADHD, attention deficit hyperactivity disorder; DNA methylation; LRR, leucine-rich repeats; OCD, obsessive–compulsive disorder; Pig; SLITRK1; TS, Tourette syndrome; Tourette