Rapid modulation of droplet composition with pincer microvalves.
Single-layer membrane valves are simple to fabricate and to integrate into microfluidic devices. However, due to their rectangular flow channel geometry, they do not fully seal. Here, we show that liquid flow can be reduced by over 3 orders of magnitude, enabling the contents of forming droplets to be dynamically modulated. We use this precision control to perform combinatorial DNA synthesis in the droplets.
Department of Bioengineering and Therapeutic Sciences, California Institute for Quantitative Biosciences, University of California San Francisco, San Francisco, California, USA. adam.abate@ucsf.edu.
Christopher J
CJ
Ochs
Adam R
AR
Abate
D004867:Equipment Design; D057446:Hydrodynamics; D008567:Membranes, Artificial; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*
D008567:Membranes, Artificial