A polystyrene-based microfluidic device with three-dimensional interconnected microporous walls for perfusion cell culture.
In this article, we present a simple, rapid prototyped polystyrene-based microfluidic device with three-dimensional (3D) interconnected microporous walls for long term perfusion cell culture. Patterned 3D interconnected microporous structures were created by a chemical treatment together with a protective mask and the native hydrophobic nature of the microporous structures were selectively made hydrophilic using oxygen plasma treatment together with a protective mask. Using this polystyrene-based cell culture microfluidic device, we successfully demonstrated the support of four days perfusion cell culture of hepatocytes (C3A cells).
Department of Engineering Science and Mechanics, The Pennsylvania State University , University Park, Pennsylvania 16802, USA.
Chung Yu
CY
Chan
Science & Technology, Corning Incorporated , Corning, New York 14831, USA.
Vasiliy N
VN
Goral
Science & Technology, Corning Incorporated , Corning, New York 14831, USA.
Michael E
ME
DeRosa
Department of Engineering Science and Mechanics, The Pennsylvania State University , University Park, Pennsylvania 16802, USA.
Tony Jun
TJ
Huang
Science & Technology, Corning Incorporated , Corning, New York 14831, USA.
Po Ki
0000-0002-8489-574X
PK
Yuen