Peptide-MHC multimer-based monitoring of CD8 T-cells in HIV-1 infection and AIDS vaccine development.
The use of MHC multimers allows precise and direct detecting and analyzing of antigen-specific T-cell populations and provides new opportunities to characterize T-cell responses in humans and animals. MHC-multimers enable us to enumerate specific T-cells targeting to viral, tumor and vaccine antigens with exceptional sensitivity and specificity. In the field of HIV/SIV immunology, this technique provides valuable information about the frequencies of HIV- and SIV-specific CD8(+) cytotoxic T lymphocytes (CTLs) in different tissues and sites of infection, AIDS progression, and pathogenesis. Peptide-MHC multimer technology remains a very sensitive tool in detecting virus-specific T -cells for evaluation of the immunogenicity of vaccines against HIV-1 in preclinical trials. Moreover, it helps to understand how immune responses are formed following vaccination in the dynamics from priming point until T-cell memory is matured. Here we review a diversity of peptide-MHC class I multimer applications for fundamental immunological studies in different aspects of HIV/SIV infection and vaccine development.
State Research Center of Virology and Biotechnology "Vector", Koltsovo, Novosibirsk region, 630559, Russia.
Alena Y
AY
Reguzova
Larisa I
LI
Karpenko
Ludmila V
LV
Mechetina
Igor M
IM
Belyakov
D016915:AIDS Vaccines / Q000276:immunology*; D000956:Antigens, Viral / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D003584:Cytological Techniques / Q000379:methods*; D005470:Fluorometry; D015658:HIV Infections / Q000276:immunology*; D015497:HIV-1 / Q000276:immunology*; D015395:Histocompatibility Antigens Class I / Q000276:immunology; D006801:Humans; D007158:Immunologic Techniques / Q000379:methods*; D011994:Recombinant Proteins / Q000276:immunology; D013194:Staining and Labeling / Q000379:methods*
D016915:AIDS Vaccines; D000956:Antigens, Viral; D015395:Histocompatibility Antigens Class I; D011994:Recombinant Proteins
AIDS; CD4+ T-cells; CD8+ CTL; CD8+ T-cells; HIV-1; MHC class I molecules; SIV; TCR; affinity; avidity; cytokines; peptide-MHC multimer; peptide-MHC tetramer; vaccine