Dynamic Electrochemical Membranes for Continuous Affinity Protein Separation.
A membrane system with nm-scale thick electrodes is able to selectively bind genetically modified proteins and pump them across the membrane with sequential voltage pulses. The electrodes are located at the first 20nm of pore entrances to specifically capture targeted proteins and block non-specific protein transport through the pores during the binding cycle. During the release cycle, concentration of imidazole is controlled to keep the pore blocked while releasing proteins at the bottom edge of the electrode. A separation factor for GFP:BSA of 16 was achieved with observed GFP electrophoretic mobility of 2.54×10-6cm2v-1S-1. This non-optimized system with a membrane area of 0.75 cm2has the same throughput as 1ml of commercially available chromatography columns showing viability as a continuous process. This system will enable continuous separation of expressed proteins directly from fermentation broths dramatically simplifying the separation process as well as reducing biopharmaceutical production costs.
Department of Chemical and Materials Engineering, University of Kentucky, Lexington, KY 40506, USA.
Zhiqiang
Z
Chen
Department of Chemical and Materials Engineering, University of Kentucky, Lexington, KY 40506, USA.
Tao
T
Chen
Department of Chemical and Materials Engineering, University of Kentucky, Lexington, KY 40506, USA.
Xinghua
X
Sun
Department of Chemical and Materials Engineering, University of Kentucky, Lexington, KY 40506, USA.
Bruce J
BJ
Hinds
Biomimetics; Nanoporous electrode; continuous separation; dynamic membrane; electrophoresis