Direct capture and heterologous expression of Salinispora natural product genes for the biosynthesis of enterocin.
Heterologous expression of secondary metabolic pathways is a promising approach for the discovery and characterization of bioactive natural products. Herein we report the first heterologous expression of a natural product from the model marine actinomycete genus Salinispora. Using the recently developed method of yeast-mediated transformation-associated recombination for natural product gene clusters, we captured a type II polyketide synthase pathway from Salinispora pacifica with high homology to the enterocin pathway from Streptomyces maritimus and successfully produced enterocin in two different Streptomyces host strains. This result paves the way for the systematic interrogation of Salinispora's promising secondary metabolome.
†Center for Marine Biotechnology and Biomedicine, Scripps Institution of Oceanography, La Jolla, California 92037, United States.
Bailey
B
Bonet
†Center for Marine Biotechnology and Biomedicine, Scripps Institution of Oceanography, La Jolla, California 92037, United States.
Robin
R
Teufel
†Center for Marine Biotechnology and Biomedicine, Scripps Institution of Oceanography, La Jolla, California 92037, United States.
Max
M
Crüsemann
†Center for Marine Biotechnology and Biomedicine, Scripps Institution of Oceanography, La Jolla, California 92037, United States.
Nadine
N
Ziemert
†Center for Marine Biotechnology and Biomedicine, Scripps Institution of Oceanography, La Jolla, California 92037, United States.|‡Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California San Diego, La Jolla, California 92093, United States.
Bradley S
BS
Moore
D039903:Actinobacteria / Q000737:chemistry / Q000235:genetics*; D001688:Biological Products / Q000737:chemistry / Q000378:metabolism*; D001952:Bridged-Ring Compounds / Q000737:chemistry / Q000378:metabolism; D055808:Drug Discovery; D008386:Marine Biology; D015394:Molecular Structure; D005810:Multigene Family; D048630:Polyketide Synthases / Q000378:metabolism; D013302:Streptomyces / Q000235:genetics*
D001688:Biological Products; D001952:Bridged-Ring Compounds; C012306:enterocin; D048630:Polyketide Synthases