Pathogen reduction in human plasma using an ultrashort pulsed laser.
Pathogen reduction is a viable approach to ensure the continued safety of the blood supply against emerging pathogens. However, the currently licensed pathogen reduction techniques are ineffective against non-enveloped viruses such as hepatitis A virus, and they introduce chemicals with concerns of side effects which prevent their widespread use. In this report, we demonstrate the inactivation of both enveloped and non-enveloped viruses in human plasma using a novel chemical-free method, a visible ultrashort pulsed laser. We found that laser treatment resulted in 2-log, 1-log, and 3-log reductions in human immunodeficiency virus, hepatitis A virus, and murine cytomegalovirus in human plasma, respectively. Laser-treated plasma showed ≥70% retention for most coagulation factors tested. Furthermore, laser treatment did not alter the structure of a model coagulation factor, fibrinogen. Ultrashort pulsed lasers are a promising new method for chemical-free, broad-spectrum pathogen reduction in human plasma.
Department of Radiology, Washington University School of Medicine, St Louis, Missouri, United States of America.
Shaw-Wei D
SW
Tsen
U. S. Department of Agriculture, Agricultural Research Service, Food Safety and Intervention Technologies Research Unit, James W. W. Baker Center, Delaware State University, Dover, Delaware, United States of America.
David H
DH
Kingsley
Biodesign Institute, Arizona State University, Tempe, Arizona, United States of America.
Karen
K
Kibler
Biodesign Institute, Arizona State University, Tempe, Arizona, United States of America.
Bert
B
Jacobs
Department of Physics, Arizona State University, Tempe, Arizona, United States of America; Center for Biophysics, Arizona State University, Tempe, Arizona, United States of America.
Sara
S
Sizemore
Department of Physics, Arizona State University, Tempe, Arizona, United States of America; Center for Biophysics, Arizona State University, Tempe, Arizona, United States of America.
Sara M
SM
Vaiana
Department of Hematology, Barnes Jewish Hospital, St Louis, Missouri, United States of America.
Jeanne
J
Anderson
Department of Physics, Arizona State University, Tempe, Arizona, United States of America; Center for Biophysics, Arizona State University, Tempe, Arizona, United States of America.
Kong-Thon
KT
Tsen
Department of Radiology, Washington University School of Medicine, St Louis, Missouri, United States of America; Biochemistry and Molecular Biophysics, Washington University School of Medicine, St Louis, Missouri, United States of America; Biomedical Engineering, Washington University School of Medicine, St Louis, Missouri, United States of America.
Samuel
S
Achilefu
D001798:Blood Proteins / Q000737:chemistry / Q000378:metabolism; D058731:Blood Safety / Q000379:methods*; D017848:Blood-Borne Pathogens / Q000528:radiation effects*; D006801:Humans; D007834:Lasers; D066263:Protein Aggregation, Pathological; D038301:Virus Inactivation / Q000528:radiation effects
D001798:Blood Proteins