Intrahepatic fat content correlates with soluble CD163 in relation to weight loss induced by Roux-en-Y gastric bypass.
OBJECTIVE
Soluble CD163 (sCD163) is a new marker of obesity-related metabolic complications. sCD163 and CD163 mRNA were investigated in relation to the fat distribution at baseline and 12 months after Roux-en-Y gastric bypass (RYGB).


METHODS
Thirty-one obese subjects (BMI: 42.3 ± 4.7 kg/m(2)) were enrolled. Subcutaneous (SAT) and visceral adipose tissue (VAT) volume were determined by MRI, intrahepatic lipid content (IHL) by MR-spectroscopy, and body composition by DXA. Fasting blood samples and adipose tissue samples were obtained, and ELISA and RT-PCR were performed.


RESULTS
RYGB-induced weight loss (36 ± 11 kg) was accompanied by a significant reduction in sCD163 (2.1 ± 0.8 mg/l vs. 1.7 ± 0.7 mg/l), SAT, VAT, and IHL (all, P < 0.001). At baseline, sCD163 was associated with VAT (r = 0.40, P < 0.05) but not with SAT or IHL. Moreover, CD163 mRNA was significantly upregulated in VAT compared with SAT at baseline (P < 0.05) and significantly downregulated in SAT after RYGB (P < 0.001). ΔsCD163 was significantly associated with ΔIHL after RYGB compared with baseline (r = 0.40, P < 0.05).


CONCLUSIONS
RYGB-induced weight loss results in a reduction of sCD163 and CD163 mRNA. The association between ΔsCD163 and ΔIHL may reflect a reduction in sCD163-producing Kupffer cells in the liver. Moreover, sCD163 may be a marker of "unhealthy" fat distribution in obese subjects.
Department of Medicine and Endocrinology MEA, Aarhus University Hospital, Aarhus, Denmark.
Karen
K
Fjeldborg
Steen B
SB
Pedersen
Holger J
HJ
Møller
Peter
P
Rask
Allan Vestergaard
AV
Danielsen
Hans
H
Stødkilde-Jørgensen
Bjørn
B
Richelsen
D050154:Adiposity / Q000502:physiology; D000328:Adult; D015703:Antigens, CD / Q000097:blood* / Q000235:genetics; D015214:Antigens, Differentiation, Myelomonocytic / Q000097:blood* / Q000235:genetics; D015415:Biomarkers / Q000097:blood; D050218:Body Fat Distribution; D005260:Female; D015390:Gastric Bypass; D006801:Humans; D050152:Intra-Abdominal Fat / Q000378:metabolism; D050356:Lipid Metabolism / Q000502:physiology*; D008099:Liver / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D009765:Obesity / Q000378:metabolism / Q000601:surgery*; D011956:Receptors, Cell Surface / Q000097:blood* / Q000235:genetics; D050151:Subcutaneous Fat / Q000378:metabolism; D015431:Weight Loss; D000098902:CD163 Antigen
D015703:Antigens, CD; D015214:Antigens, Differentiation, Myelomonocytic; D015415:Biomarkers; D000098902:CD163 Antigen; D011956:Receptors, Cell Surface