Inhibition of fumonisin B1 cytotoxicity by nanosilicate platelets during mouse embryo development.
Nanosilicate platelets (NSP), the form of natural silicate clay that was exfoliated from montmorillonite (MMT), is widely used as a feed additive for its high non-specific binding capacity with mycotoxins such as fumonisin B1 (FB1), and has been evaluated its safety for biomedical use including cytotoxicity, genotoxicity, and lethal dosage (LD). In the study, we further examined its toxicity on the development of CD1 mouse embryos and its capacity to prevent teratogenesis-induced by FB1. In vitro cultures, NSP did not disturb the development and the quality of intact pre-implantation mouse embryos. Further, newborn mice from females consumed with NSP showed no abnormalities. NSP had an unexpected high adsorption capacity in vitro. In contrast to female mice consumed with FB1 only, a very low residual level of FB1 in the circulation, reduced incidence of neutral tube defects and significantly increased fetal weight were observed in the females consumed with FB1 and NSP, suggesting a high alleviation effect of NSP on FB1 in vivo. Furthermore, FB1 treatment disturbed the gene expression of sphingolipid metabolism enzymes (longevity assurance homolog 5, LASS 5; sphingosine kinase 1, Sphk1; sphingosine kinase 2, Sphk2; sphingosine 1- phosphate lyase, Sgpl1; sphingosine 1-phosphate phosphatase, Sgpp1) in the maternal liver, uterus, fetus, and placenta, but NSP administration reversed the perturbations. Based on these findings, we conclude that NSP is a feasible and effective agent for supplementary use in reducing the toxicity of FB1 to animals.
Division of Physiology, Livestock Research Institute, Council of Agriculture Executive Yuan, Tainan, Taiwan; Department of Animal Science, National Chung Hsing University, Taichung, Taiwan.
Yu-Jing
YJ
Liao
Division of Physiology, Livestock Research Institute, Council of Agriculture Executive Yuan, Tainan, Taiwan.
Jenn-Rong
JR
Yang
Department of Animal Science, National Chung Hsing University, Taichung, Taiwan; Agricultural Biotechnology Center, National Chung Hsing University, Taichung, Taiwan; Center for the Integrative and Evolutionary Galliformes Genomics, National Chung Hsing University, Taichung, Taiwan.
Shuen-Ei
SE
Chen
Department of Animal Science, National Chung Hsing University, Taichung, Taiwan.
Sing-Jhou
SJ
Wu
Department of Animal Science, National Chung Hsing University, Taichung, Taiwan; Agricultural Biotechnology Center, National Chung Hsing University, Taichung, Taiwan; Center for the Integrative and Evolutionary Galliformes Genomics, National Chung Hsing University, Taichung, Taiwan; Center of Nanoscience and Nanotechnology, National Chung Hsing University, Taichung, Taiwan.
San-Yuan
SY
Huang
Institute of Polymer Science and Engineering, National Taiwan University, Taipei, Taiwan.
Jiang-Jen
JJ
Lin
Division of Physiology, Livestock Research Institute, Council of Agriculture Executive Yuan, Tainan, Taiwan.
Lih-Ren
LR
Chen
Department of Animal Science, National Chung Hsing University, Taichung, Taiwan; Agricultural Biotechnology Center, National Chung Hsing University, Taichung, Taiwan; Center for the Integrative and Evolutionary Galliformes Genomics, National Chung Hsing University, Taichung, Taiwan.
Pin-Chi
PC
Tang
D000327:Adsorption; D000818:Animals; D001755:Blastocyst / Q000187:drug effects; D003603:Cytotoxins / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000633:toxicity; D047108:Embryonic Development / Q000187:drug effects*; D005260:Female; D037341:Fumonisins / Q000037:antagonists & inhibitors* / Q000737:chemistry / Q000633:toxicity*; D008297:Male; D051379:Mice; D049329:Nanostructures; D017640:Silicates / Q000737:chemistry* / Q000494:pharmacology*
D003603:Cytotoxins; D037341:Fumonisins; D017640:Silicates; C056933:fumonisin B1