Silver nanoparticle toxicity to Atlantic killifish (Fundulus heteroclitus) and Caenorhabditis elegans: a comparison of mesocosm, microcosm, and conventional laboratory studies.
The use of silver nanoparticles (AgNPs) in consumer products and industrial applications, as well as their recent detection in waste streams, has created concern about potential impacts on aquatic ecosystems. The effect of complex environmental media on AgNP toxicity was investigated using wetland mesocosms and smaller scale microcosms. Mesocosms were dosed with 2.5 mg Ag/L as gum arabic (GA)-coated AgNPs, polyvinylpyrrolidone (PVP)-coated AgNPs, or AgNO3. Water samples were taken from mesocosms 24 h after dosing for acute toxicity tests with embryos and larvae of Atlantic killifish (Fundulus heteroclitus) and the nematode Caenorhabditis elegans. Acute toxicity tests were also performed on Atlantic killifish with AgNO3, GA AgNPs, and PVP AgNPs prepared in the laboratory with similar water. For killifish embryos, mesocosm samples were much less toxic than laboratory samples for all types of silver. For larvae, in contrast, all 3 silver mesocosm treatments exhibited toxicity. Interestingly, mesocosm samples of AgNO3 were less toxic than laboratory samples; samples containing GA AgNPs were similar in toxicity, and samples containing PVP AgNPs were more toxic. For C. elegans, results were similar to killifish larvae. Results obtained from the mesocosms were not replicated on the smaller scale of the microcosms. These results indicate that environmental factors unique to the mesocosms acted differentially on AgNO3 to reduce its toxicity in a manner that does not translate to AgNPs for larval fish.
Center for the Environmental Implications of NanoTechnology (CEINT), Duke University, Durham, North Carolina, USA; Nicholas School of the Environment, Duke University, Durham, North Carolina, USA.
Audrey J
AJ
Bone
Cole W
CW
Matson
Benjamin P
BP
Colman
Xinyu
X
Yang
Joel N
JN
Meyer
Richard T
RT
Di Giulio
D000818:Animals; D017173:Caenorhabditis elegans / Q000187:drug effects*; D004625:Embryo, Nonmammalian / Q000187:drug effects; D023781:Fundulidae / Q000378:metabolism*; D007753:Laboratories; D007814:Larva / Q000187:drug effects; D053768:Metal Nanoparticles / Q000633:toxicity*; D012834:Silver / Q000633:toxicity*; D018675:Toxicity Tests
D012834:Silver
Aquatic toxicology; Ecotoxicology; Mesocosms; Microcosms; Nanotoxicology