Synthesis of a Cu²⁺-selective probe derived from rhodamine and its application in cell imaging.
A new fluorescent probe P based on rhodamine for Cu2+ was synthesized and characterized. The new probe P showed high selectivity to Cu2+ over other tested metal ions. With optimal conditions, the proposed probe P worked in a wide linear range of 1.0 × 10(-6)-1.0 × 10(-5) M with a detection limit of 3.3 × 10(-7) M Cu2+ in ethanol-water solution (9:1, v:v, 20 mM HEPES, pH 7.0). Furthermore, it has been used for imaging of Cu2+ in living cells with satisfying results.
Laboratory of Environmental Monitoring, School of Tropical and Laboratory Medicine, Hainan Medical University, Haikou 571199, China. yucw_1979@sina.com.
Chunwei
C
Yu
Laboratory of Environmental Monitoring, School of Tropical and Laboratory Medicine, Hainan Medical University, Haikou 571199, China. wyy_418@163.com.
Yingying
Y
Wen
Laboratory of Environmental Monitoring, School of Tropical and Laboratory Medicine, Hainan Medical University, Haikou 571199, China. jun_zh1979@163.com.
Jun
J
Zhang
D003300:Copper / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D056945:Hep G2 Cells; D006801:Humans; D007089:Image Enhancement / Q000379:methods*; D008856:Microscopy, Fluorescence / Q000379:methods*; D015203:Reproducibility of Results; D012235:Rhodamines / Q000737:chemistry*; D012680:Sensitivity and Specificity; D013347:Subcellular Fractions / Q000648:ultrastructure*
D005456:Fluorescent Dyes; D012235:Rhodamines; D003300:Copper