Relationship between gelatin concentrations in silk fibroin-based composite scaffolds and adhesion and proliferation of mouse embryo fibroblasts.
Porous scaffolds of silk fibroin and composite porous scaffolds with 10, 20, 30, 40, and 50% gelatin were made by the freezing-thawing method. The relationship between adhesion and proliferation rate mouse embryo fibroblast and the scaffold composition was studied by laser confocal scanning microscopy. Addition of gelatin to the scaffold structure stimulated adhesion and proliferation of mouse embryo fibroblasts; the optimal content of gelatin was 30%.
Biological Faculty, M. V. Lomonosov Moscow State University, Moscow, Russia, orlovaselin@gmail.com.
A A
AA
Orlova
M S
MS
Kotlyarova
V S
VS
Lavrenov
S V
SV
Volkova
A Yu
AY
Arkhipova
D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D002448:Cell Adhesion; D049109:Cell Proliferation; D002470:Cell Survival; D002478:Cells, Cultured; D003470:Culture Media; D005260:Female; D005347:Fibroblasts / Q000502:physiology*; D005349:Fibroins / Q000737:chemistry*; D005780:Gelatin / Q000737:chemistry*; D008810:Mice, Inbred C57BL; D016062:Porosity; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry*
D001672:Biocompatible Materials; D003470:Culture Media; C482288:fibroin, silkworm; D005780:Gelatin; D005349:Fibroins