Microfluidic chip with thermoresponsive boronate affinity for the capture-release of cis-diol biomolecules.
The polydimethylsiloxane microfluidic chip grafted with poly(N-isopropylacrylamide-3-acrylamidephenylboronic acid) (P(NIPAAm-co-AAPBA)) was fabricated by UV-induced grafting polymerization for the capture-release of cis-diol-containing biomolecules by temperature-modulated changes instead of changing the pH value of the mobile phase. Based on the optimal time for benzophenone soaking and UV irradiation of grafting polymerization, P(NIPAAm-co-AAPBA) was successfully grafted on the polydimethylsiloxane substrates, which were characterized by scanning electron microscopy, water contact angle measurements, and Fourier transform infrared spectroscopy. The P(NIPAAm-co-AAPBA)-grafted polydimethylsiloxane microfluidic chip can be successfully used for the capture and release of cis-diol-containing adenosine by adjusting the temperature from 4 to 55°C, and the result was validated by Triple Quad liquid chromatography with mass spectrometry. With further development, the fabricated polydimethylsiloxane microfluidic chips might be chosen as a potential tool for the capture and release of cis-diol-containing macromolecules, such as horseradish peroxidase and glycoprotein.
School of Life Science, Beijing Institute of Technology, Beijing, China.
Yan
Y
Man
Guang
G
Peng
Jianshe
J
Wang
Xuefei
X
Lv
Yulin
Y
Deng
D001897:Boronic Acids / Q000737:chemistry*; D004129:Dimethylpolysiloxanes; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D008855:Microscopy, Electron, Scanning; D017550:Spectroscopy, Fourier Transform Infrared; D013696:Temperature
D001897:Boronic Acids; D004129:Dimethylpolysiloxanes; C013830:baysilon
Boronate affinity; Poly(N-isopropylacrylamide); Polydimethylsiloxane; Thermoresponse