Expression, purification, crystallization and preliminary X-ray crystallographic analysis of the extracellular olfactomedin domain of gliomedin.
Gliomedin (GLDN) is one of the essential proteins in the development of the nodes of Ranvier in the vertebrate peripheral nervous system. An olfactomedin (OLF) domain is located at the GLDN extracellular C-terminus and is involved in the accumulation of neuronal plasma membrane voltage-gated sodium channels in the nodes by interacting with neurofascin and NrCAM. No structures of OLF domains have previously been reported. Here, the crystallization of the rat GLDN OLF domain, which was expressed in an insect-cell system, is reported. The crystal diffracted to 1.55 Å resolution and belonged to space group P2₁, with unit-cell parameters a=37.5, b=141.7, c=46.0 Å, β=110.6°, and had two molecules in the asymmetric unit.
Faculty of Biochemistry and Molecular Medicine and Biocenter Oulu, University of Oulu, PO Box 3000, FIN-90014 Oulu, Finland.
Huijong
H
Han
Faculty of Biochemistry and Molecular Medicine and Biocenter Oulu, University of Oulu, PO Box 3000, FIN-90014 Oulu, Finland.
Petri
P
Kursula
D000595:Amino Acid Sequence; D000818:Animals; D015816:Cell Adhesion Molecules, Neuronal / Q000096:biosynthesis* / Q000737:chemistry* / Q000302:isolation & purification; D002460:Cell Line; D003460:Crystallization; D018360:Crystallography, X-Ray; D016326:Extracellular Matrix Proteins / Q000096:biosynthesis* / Q000737:chemistry* / Q000302:isolation & purification; D005786:Gene Expression Regulation; D006023:Glycoproteins / Q000096:biosynthesis* / Q000737:chemistry* / Q000302:isolation & purification; D007313:Insecta; D008969:Molecular Sequence Data; D017434:Protein Structure, Tertiary / Q000502:physiology; D051381:Rats
D015816:Cell Adhesion Molecules, Neuronal; D016326:Extracellular Matrix Proteins; D006023:Glycoproteins; C504359:gliomedin, rat; C081451:olfactomedin
Schwann cell; cell adhesion; gliomedin; myelin; olfactomedin