Effects of hypothermia combined with neural stem cell transplantation on recovery of neurological function in rats with spinal cord injury.
The microenvironment of the injured spinal cord is hypothesized to be involved in driving the differentiation and survival of engrafted neural stem cells (NSCs). Hypothermia is known to improve the microenvironment of the injured spinal cord in a number of ways. To investigate the effect of NSC transplantation in combination with hypothermia on the recovery of rat spinal cord injury, 60 Sprague‑Dawley female rats were used to establish a spinal cord hemisection model. They were divided randomly into three groups: A, spinal cord injury group; B, NSC transplantation group; and C, NSC transplantation + hypothermia group. At 1, 2, 4, 6 and 8 weeks post‑injury, the motor function of all animals was evaluated using the Basso, Beattie and Besnaham locomotor scoring system and the inclined plane test. At 4 weeks post‑transplantation, histological analysis and immunocytochemistry were performed. At 8 weeks post‑transplantation, horseradish peroxidase nerve tracing and transmission electron microscopy were conducted to observe axonal regeneration. The outcome of hind limb motor function recovery in group C significantly surpassed that in group B at 4 weeks post‑injury (P<0.05). Recovery was also observed in group A, but to a lesser degree. For the pathological sections no neural axonal were observed in group A. A few axon‑like structures were observed in group B and more in group C. Horseradish peroxidase‑labeled neurofibers and bromodeoxyuridine‑positive cells were observed in the spinal cords of group C. Fewer of these cells were found in group B and fewer still in group A. The differences among the three groups were significant (P<0.05). Using transmission electron microscopy, newly formed nerve fibers and myelinated nerve fibers were observed in the central transverse plane in groups B and C, although these nerve fibers were not evident in group A. In conclusion, NSC transplantation promoted the recovery of hind limb function in rats, and combination treatment with hypothermia produced synergistic effects.
Department of Neurosurgery, The Fourth Center Clinical College of Tianjin Medical University, Tianjin 300140, P.R. China.
Dong
D
Wang
Department of Neurosurgery, The Fourth Center Clinical College of Tianjin Medical University, Tianjin 300140, P.R. China.
Jianjun
J
Zhang
D000818:Animals; D001253:Astrocytes / Q000378:metabolism / Q000473:pathology; D001692:Biological Transport; D001812:Blood-Brain Barrier / Q000378:metabolism; D004195:Disease Models, Animal; D005260:Female; D007036:Hypothermia, Induced / Q000379:methods; D007150:Immunohistochemistry; D059630:Mesenchymal Stem Cells / Q000166:cytology; D058953:Neural Stem Cells / Q000166:cytology*; D009474:Neurons / Q000166:cytology / Q000378:metabolism; D051381:Rats; D020127:Recovery of Function; D013119:Spinal Cord Injuries / Q000378:metabolism / Q000473:pathology / Q000628:therapy*; D033581:Stem Cell Transplantation