Glypican 4 may be involved in the adipose tissue redistribution in high-fat feeding C57BL/6J mice with peroxisome proliferators-activated receptor γ agonist rosiglitazone treatment.
Fat distribution affects the risk of developing obesity-related chronic diseases. Glypican 4 (Gpc4) may be involved in the regulation of obesity and body fat distribution. The aim of the study was to explore whether Gpc4 affects fat accumulation and the possible mechanism. C57BL/6J mice were fed with a high-fat diet for eight weeks and treated with a peroxisome proliferators-activated receptor γ (PPARγ) agonist, rosiglitazone, for another four weeks. The weight of inguinal and epididymal fat pads was determined. TheGpc4mRNA and protein expression and two probable regulators of theGpc4gene, specificity protein 1 (Sp1) andSp3mRNA, were also measured. Mice treated with rosiglitazone showed a significant increase in subcutaneous fat weight compared with the untreated mice. The expression ofGpc4mRNA and protein was significantly higher in visceral than in subcutaneous fat in all the groups. Compared with untreated mice the expression ofGpc4andSp3mRNA in subcutaneous fat and the expression ofSp1andSp3mRNA in visceral fat in mice treated with rosiglitazone increased significantly. TheSp3/Sp1ratio was consistent with the expression ofGpc4mRNA and protein in subcutaneous and visceral fat. The present study indicated thatGpc4may play an important role in fat distribution, and this effect is perhaps regulated by the ratio of Sp3/Sp1 in the subcutaneous and visceral fat tissues.
Department of Nutrition and Food Hygiene, School of Public Health, China Medical University, Shenyang, Liaoning 110001, P.R. China.
Li
L
Liu
Department of Orthopedics, Shengjing Hospital, China Medical University, Shenyang, Liaoning 110004, P.R. China.
Hailun
H
Gu
Department of Nutrition and Food Hygiene, School of Public Health, China Medical University, Shenyang, Liaoning 110001, P.R. China.
Yue
Y
Zhao
Department of Nutrition and Food Hygiene, School of Public Health, China Medical University, Shenyang, Liaoning 110001, P.R. China.
Li
L
An
Department of Nutrition and Food Hygiene, School of Public Health, China Medical University, Shenyang, Liaoning 110001, P.R. China.
Jun
J
Yang
glypican 4; obesity; peroxisome proliferators-activated receptor γ; subcutaneous fat; visceral fat