Description of Kribbella italica sp. nov., isolated from a Roman catacomb.
A novel actinobacterium, strain BC637(T), was isolated from a biodeteriogenic biofilm sample collected in 2009 in the Saint Callixstus Roman catacomb. The strain was found to belong to the genus Kribbella by analysis of the 16S rRNA gene. Phylogenetic analysis using the 16S rRNA gene and the gyrB, rpoB, relA, recA and atpD concatenated gene sequences showed that strain BC637(T) was most closely related to the type strains of Kribbella lupini and Kribbella endophytica. DNA-DNA hybridization experiments confirmed that strain BC637(T) is a genomic species that is distinct from its closest phylogenetic relatives, K. endophytica DSM 23718(T) (63 % DNA relatedness) and K. lupini LU14(T) (63 % DNA relatedness). Physiological comparisons showed that strain BC637(T) is phenotypically distinct from the type strains of K. endophytica and K. lupini. Thus, strain BC637(T) represents the type strain of a novel species, for which the name Kribella italica sp. nov. is proposed ( = DSM 28967(T) = NRRL B-59155(T)).
Department of Molecular and Cell Biology, University of Cape Town, Private Bag X3, Rondebosch, 7701 Cape Town, South Africa.
Gareth J
GJ
Everest
Department of Molecular and Cell Biology, University of Cape Town, Private Bag X3, Rondebosch, 7701 Cape Town, South Africa.
Sarah M
SM
Curtis
Department of Biological and Environmental Sciences, University of Messina, Viale F. Stagno d'Alcontres 31, I-98166 Messina, Italy.
Filomena
F
De Leo
Department of Biological and Environmental Sciences, University of Messina, Viale F. Stagno d'Alcontres 31, I-98166 Messina, Italy.
Clara
C
Urzì
Department of Molecular and Cell Biology, University of Cape Town, Private Bag X3, Rondebosch, 7701 Cape Town, South Africa.
Paul R
PR
Meyers
D000192:Actinomycetales / Q000145:classification*; D015373:Bacterial Typing Techniques; D018441:Biofilms; D059552:Caves / Q000382:microbiology*; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010457:Peptidoglycan / Q000737:chemistry; D010713:Phosphatidylcholines / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012384:Rome; D017422:Sequence Analysis, DNA; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
D004269:DNA, Bacterial; D005227:Fatty Acids; D010457:Peptidoglycan; D010713:Phosphatidylcholines; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C073355:menaquinone 9