Functional adaptations of the bacterial chaperone trigger factor to extreme environmental temperatures.
Trigger factor (TF) is the first molecular chaperone interacting cotranslationally with virtually all nascent polypeptides synthesized by the ribosome in bacteria. Thermal adaptation of chaperone function was investigated in TFs from the Antarctic psychrophile Pseudoalteromonas haloplanktis, the mesophile Escherichia coli and the hyperthermophile Thermotoga maritima. This series covers nearly all temperatures encountered by bacteria. Although structurally homologous, these TFs display strikingly distinct properties that are related to the bacterial environmental temperature. The hyperthermophilic TF strongly binds model proteins during their folding and protects them from heat-induced misfolding and aggregation. It decreases the folding rate and counteracts the fast folding rate imposed by high temperature. It also functions as a carrier of partially folded proteins for delivery to downstream chaperones ensuring final maturation. By contrast, the psychrophilic TF displays weak chaperone activities, showing that these functions are less important in cold conditions because protein folding, misfolding and aggregation are slowed down at low temperature. It efficiently catalyses prolyl isomerization at low temperature as a result of its increased cellular concentration rather than from an improved activity. Some chaperone properties of the mesophilic TF possibly reflect its function as a cold shock protein in E. coli.
Laboratory of Biochemistry, Centre for Protein Engineering, University of Liège, Liège, B-4000, Belgium.
Amandine
A
Godin-Roulling
Laboratorium für Biochemie, Bayreuther Zentrum für Molekulare Biowissenschaften, Universität Bayreuth, Bayreuth, D-95447, Germany.
Philipp A M
PA
Schmidpeter
Laboratorium für Biochemie, Bayreuther Zentrum für Molekulare Biowissenschaften, Universität Bayreuth, Bayreuth, D-95447, Germany.
Franz X
FX
Schmid
Laboratory of Biochemistry, Centre for Protein Engineering, University of Liège, Liège, B-4000, Belgium.
Georges
G
Feller
D000064:Acclimatization; D001426:Bacterial Proteins / Q000378:metabolism; D057773:Cold Shock Proteins and Peptides / Q000378:metabolism*; D003080:Cold Temperature; D004926:Escherichia coli / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000378:metabolism; D018832:Molecular Chaperones / Q000378:metabolism*; D019696:Peptidylprolyl Isomerase / Q000378:metabolism; D011485:Protein Binding; D017510:Protein Folding; D031161:Pseudoalteromonas / Q000378:metabolism*; D012270:Ribosomes / Q000378:metabolism; D013696:Temperature; D020124:Thermotoga maritima / Q000378:metabolism*
D001426:Bacterial Proteins; D057773:Cold Shock Proteins and Peptides; D029968:Escherichia coli Proteins; D018832:Molecular Chaperones; C095034:trigger factor, Campylobacter; D019696:Peptidylprolyl Isomerase