Anti-oxidant effects of pomegranate juice on Saccharomyces cerevisiae cell growth.
BACKGROUND
Pomegranate juice has a number of positive effects on both human and animal subjects.


METHODS
Four groups were used in this study. i: Control group, ii: H2O2 group, iii: Pomegranate juice (PJ) group and iv: PJ + H2O2 group. Following the sterilization method for pomegranate juice (10%) and H2O2 (6% v/v), Saccharomyces cerevisiae cultures were added and the cultivation incubated at 35°C for 72 hours. Fatty acids and vitamin concentrations were measured using HPLC and GC and the total protein bands profile were determined by SDS-PAGE.


RESULTS
According to our results statistically significant differences have been determined among the study groups in terms of fatty acids and vitamin (p<0,05). Fatty acid synthesis, vitamin control and cell density increased in groups to which PJ was given in comparison with the control group (p<0,05). Pomegranate juice increased vitamins, fatty acids and total protein expression in Saccharomyces cerevisiae in comparison with the control.


CONCLUSIONS
Pomegranate juice has a positive effect on fatty acid, vitamin and protein synthesis by Saccharomyces cerevisiae. Accordingly, we believe that it has significantly decreased oxidative damage thereby making a positive impact on yeast development.
Firat University, Faculty of Science, Department of Biology, Elazığ-TURKEY.
Abdullah
A
Aslan
Firat University, Faculty of Science, Department of Biology, Elazığ-TURKEY.
Muhammed İsmail
Mİ
Can
Firat University, Faculty of Science, Department of Biology, Elazığ-TURKEY.
Didem
D
Boydak
D000975:Antioxidants / Q000494:pharmacology*; D001628:Beverages; D049109:Cell Proliferation / Q000187:drug effects*; D005227:Fatty Acids / Q000096:biosynthesis; D006861:Hydrogen Peroxide; D015227:Lipid Peroxidation / Q000187:drug effects; D029561:Lythraceae; D018384:Oxidative Stress / Q000187:drug effects*; D010936:Plant Extracts / Q000494:pharmacology*; D014176:Protein Biosynthesis / Q000187:drug effects; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D014815:Vitamins / Q000378:metabolism
D000975:Antioxidants; D005227:Fatty Acids; D010936:Plant Extracts; D014815:Vitamins; D006861:Hydrogen Peroxide
Pomegranate juice; SDS-PAGE; fatty acid; vitamin