Evaluation of the antibacterial activity of cystatin against selected strains of Escherichia coli.
The aim of this study was to analyze the antibacterial activity of hen egg white cystatin against selected Escherichia coli strains. We used a monomeric solution of hen egg white cystatin in bovine serum albumin (BSA) with added phosphate buffered saline (PBS), and three test strains: Escherichia coli ATCC 23811, Escherichia coli ATCC 8739 and Escherichia coli ATCC 25922. The effect of cystatin against the tested strains was determined on the basis of minimal inhibitory concentration (MIC) and survival curves of the microorganisms in a cystatin-containing environment during incubation at various temperatures. Our study confirmed the activity of cystatin against the analyzed Escherichia coli strains. taining environment, as compared to control samples incubated in a ovocystatin-deficient medium. Depending on the incubation temperature (20 degrees C or 37 degrees C) the reduction persisted up to 12 hours after incubation.
Maciej
M
Szpak
Tadeusz
T
Trziszka
Antoni
A
Polanowski
Jakub
J
Gburek
Krzysztof
K
Gołąb
Katarzyna
K
Juszczyńska
Paulina
P
Janik
Adam
A
Malicki
Katarzyna
K
Szyplik
D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology*; D002645:Chickens; D004529:Egg White / Q000737:chemistry; D004926:Escherichia coli / Q000145:classification* / Q000187:drug effects*; D010455:Peptides / Q000737:chemistry / Q000494:pharmacology; D013997:Time Factors
D000900:Anti-Bacterial Agents; D010455:Peptides; C072752:ovocystatin