Regulatory role of tetR gene in a novel gene cluster of Acidovorax avenae subsp. avenae RS-1 under oxidative stress.
Acidovorax avenae subsp. avenae is the causal agent of bacterial brown stripe disease in rice. In this study, we characterized a novel horizontal transfer of a gene cluster, including tetR, on the chromosome of A. avenae subsp. avenae RS-1 by genome-wide analysis. TetR acted as a repressor in this gene cluster and the oxidative stress resistance was enhanced in tetR-deletion mutant strain. Electrophoretic mobility shift assay demonstrated that TetR regulator bound directly to the promoter of this gene cluster. Consistently, the results of quantitative real-time PCR also showed alterations in expression of associated genes. Moreover, the proteins affected by TetR under oxidative stress were revealed by comparing proteomic profiles of wild-type and mutant strains via 1D SDS-PAGE and LC-MS/MS analyses. Taken together, our results demonstrated that tetR gene in this novel gene cluster contributed to cell survival under oxidative stress, and TetR protein played an important regulatory role in growth kinetics, biofilm-forming capability, superoxide dismutase and catalase activity, and oxide detoxicating ability.
State Key Laboratory of Rice Biology, Institute of Biotechnology, Zhejiang University Hangzhou, China ; Department of Plant Pathology, University of California Davis Davis, CA, USA.
He
H
Liu
State Key Laboratory of Rice Biology, Institute of Biotechnology, Zhejiang University Hangzhou, China.
Chun-Lan
CL
Yang
State Key Laboratory of Rice Biology, Institute of Biotechnology, Zhejiang University Hangzhou, China.
Meng-Yu
MY
Ge
State Key Laboratory of Rice Biology, Institute of Biotechnology, Zhejiang University Hangzhou, China ; Department of Biosciences, COMSATS Institute of Information Technology Sahiwal, Pakistan.
Muhammad
M
Ibrahim
State Key Laboratory of Rice Biology, Institute of Biotechnology, Zhejiang University Hangzhou, China.
Bin
B
Li
Chinese Academy of Inspection and Quarantine Beijing, China.
Wen-Jun
WJ
Zhao
School of Agriculture and Biology, Shanghai Jiao Tong University Shanghai, China.
Gong-You
GY
Chen
State Key Laboratory of Rice Biology, Institute of Biotechnology, Zhejiang University Hangzhou, China.
Bo
B
Zhu
State Key Laboratory of Rice Biology, Institute of Biotechnology, Zhejiang University Hangzhou, China.
Guan-Lin
GL
Xie
EMSA; horizontal gene transfer; oxidative stress; proteome profiling; repressor; tetR