Monitoring cerebral hemodynamics following optogenetic stimulation via optical coherence tomography.
In this article, spectral domain optical coherence tomography is used to measure the hemodynamic response induced by optogenetic stimulation in the somatosensory cortex of transgenic mice. By analyzing the 3-D angiograms and Doppler measurements produced by coherence tomography, we observed significant increase in blood flow as a result of increased vessel diameter and blood velocity following optical stimulation of cortical neurons. Such distinct responses were not observed in control experiments where the brain of wild-type mice were exposed to the same light pulses.
Farid
F
Atry
Seth
S
Frye
Thomas J
TJ
Richner
Sarah K
SK
Brodnick
Alana
A
Soehartono
Justin
J
Williams
Ramin
R
Pashaie
D000818:Animals; D001783:Blood Flow Velocity / Q000502:physiology*; D001921:Brain / Q000502:physiology*; D002533:Cerebral Angiography / Q000295:instrumentation; D002560:Cerebrovascular Circulation / Q000502:physiology*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007834:Lasers; D051379:Mice; D062308:Optogenetics / Q000295:instrumentation*; D010775:Photic Stimulation / Q000295:instrumentation*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D041623:Tomography, Optical Coherence / Q000295:instrumentation*