Systemic host response following skin burn injury in rats: cytotoxicity and genotoxicity evaluation.
OBJECTIVE
The aim of the present study was to investigate whether skin burn injury (BI) can induce cellular changes in skeletal muscle, liver, kidney and blood by means of DNA damage and cytotoxicity.


METHODS
Thirty male Wistar rats were distributed into two groups: control (C) and submitted to scald burn (SB), subdivided into three subgroups: 1, 4 or 14 days post-injury. The gastrocnemius muscle and liver were dissected for histopathological evaluation and the single-cell gel (comet) assay was used to investigate damage in skeletal muscle, liver, kidney and blood cells.


RESULTS
Histopathological analysis of the muscle in the SB group revealed congested vessels containing inflammatory cells for all periods evaluated post-injury. In liver, the one day post-injury SB group showed sinusoidal congestion, while that of 14 days post-injury exhibited an increased number of Kupffer cells.


CONCLUSIONS
Despite the histophatological evidence, none of the groups showed any signs of genotoxicity in these target tissues.
Department of Biosciences, Federal University of São Paulo (UNIFESP), São Paulo, Brazil.
Hananiah Tardivo
HT
Quintana
Department of Biosciences, Federal University of São Paulo (UNIFESP), São Paulo, Brazil.
Jeferson André
JA
Bortolin
Department of Biosciences, Federal University of São Paulo (UNIFESP), São Paulo, Brazil.
Daniel Araki
DA
Ribeiro
Department of Biosciences, Federal University of São Paulo (UNIFESP), São Paulo, Brazil flavia.oliveira@unifesp.br.
Flavia
F
De Oliveira
D000818:Animals; D002056:Burns / Q000378:metabolism* / Q000473:pathology; D004249:DNA Damage; D007668:Kidney / Q000378:metabolism / Q000473:pathology; D008099:Liver / Q000378:metabolism / Q000473:pathology; D008297:Male; D018482:Muscle, Skeletal / Q000378:metabolism / Q000473:pathology; D051381:Rats; D012871:Skin Diseases / Q000378:metabolism* / Q000473:pathology
burn injury; genetic damage; histopathology; rat