microRNA-195-Cdc42 axis acts as a prognostic factor of esophageal squamous cell carcinoma.
OBJECTIVE
Previous studies observed the downregulation of microRNA (miR)-195 in esophageal squamous cell carcinoma (ESCC) tissues, confirmed cell division cycle 42 (Cdc42) as one target gene of miR-195, and demonstrated that miR-195 may act as a tumor suppressor in ESCC by regulating Cdc42 expression. This study aimed to explore the association of miR-195 and Cdc42 combined expression with clinicopathologic factors and prognosis.


METHODS
Expression of miR-195 and Cdc42 mRNA in 98 pairs of ESCC and paracancerous tissues were detected using real-time quantitative RT-PCR.


RESULTS
miR-195 downregulation and Cdc42 upregulation were both prevalent in ESCC tissues, and negatively correlated with each other. In addition, miR-195 expression negatively correlated with TNM stage (P=0.008) and lymphatic metastasis (P=0.022), while Cdc42 expression positively correlated with TNM stage (P=0.011) and tumor differentiation (P=0.024). Moreover, combined expression of miR-195 and Cdc42 (miR-195/Cdc42) was found to be prognostic indicators for progression-free survival and overall survival of ESCC patients both in univariate and multivariate analyses.


CONCLUSIONS
The main findings of this study indicate the involvement of miR-195-Cdc42 axis in the progression of ESCC and suggest that the combined aberrant expression of miR-195 and Cdc42 mRNA can serve as a promising unfavorable prognostic biomarker in ESCC.
School of Nursing, Xinxiang Medical University Xinxiang 453003, China.
Niuniu
N
Sun
School of Nursing, Xinxiang Medical University Xinxiang 453003, China.
Lin
L
Ye
The First Affiliated Hospital of Xinxiang Medical University Xinxiang 453003, China.
Tingmin
T
Chang
School of Nursing, Xinxiang Medical University Xinxiang 453003, China ; Henan Nursing Vocational College Anyang 455000, China.
Xiaojing
X
Li
Center for Cancer Research, School of Nursing, Xinxiang Medical University Xinxiang 453003, China ; The Third Affiliated Hospital of Xinxiang Medical University Xinxiang 453003, China.
Xiumin
X
Li
D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000235:genetics*; D002294:Carcinoma, Squamous Cell / Q000201:enzymology / Q000235:genetics* / Q000401:mortality / Q000473:pathology / Q000628:therapy; D002454:Cell Differentiation; D016009:Chi-Square Distribution; D018450:Disease Progression; D018572:Disease-Free Survival; D004938:Esophageal Neoplasms / Q000201:enzymology / Q000235:genetics* / Q000401:mortality / Q000473:pathology / Q000628:therapy; D000077277:Esophageal Squamous Cell Carcinoma; D005260:Female; D015971:Gene Expression Regulation, Enzymologic; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D053208:Kaplan-Meier Estimate; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D015999:Multivariate Analysis; D009367:Neoplasm Staging; D016016:Proportional Hazards Models; D012333:RNA, Messenger / Q000032:analysis; D060888:Real-Time Polymerase Chain Reaction; D012307:Risk Factors; D013997:Time Factors; D020764:cdc42 GTP-Binding Protein / Q000235:genetics*
D014408:Biomarkers, Tumor; C552722:MIRN195 microRNA, human; D035683:MicroRNAs; D012333:RNA, Messenger; D020764:cdc42 GTP-Binding Protein
cell division cycle 42; esophageal squamous cell carcinoma; microRNA-195; prognosis; real-time quantitative PCR