A new PANI biosensor based on catalase for cyanide determination.
Cyanide is one of the most widespread of compounds measured in environmental analysis due to their toxic effects on environment and health. We report a highly sensitive, reliable, selective amperometric sensor for determination of cyanide, using a polyaniline conductive polymer. The enzyme catalase was immobilized by electropolymerization. The steps during the immobilization were controlled by electrochemical impedance spectroscopy. Optimum pH, temperature, aniline concentration, enzyme concentration, and the number of scans obtained during electropolymerization, were investigated. In addition, the cyanide present in artificial waste water samples was determined. In the characterization studies of the biosensor, some parameters such as reproducibility and storage stability, were analyzed.
a Department of Chemistry , Faculty of Science, Trakya Üniversity , Edirne , Turkey.
Hakkı Mevlüt
HM
Özcan
Tuba
T
Aydin
D000814:Aniline Compounds / Q000737:chemistry*; D000818:Animals; D015374:Biosensing Techniques / Q000379:methods*; D002374:Catalase / Q000737:chemistry / Q000378:metabolism*; D002417:Cattle; D003486:Cyanides / Q000032:analysis* / Q000737:chemistry; D004553:Electric Conductivity; D004563:Electrochemistry; D004566:Electrodes; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism; D005976:Glutaral / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D057230:Limit of Detection; D016014:Linear Models; D058105:Polymerization; D015203:Reproducibility of Results; D013696:Temperature; D013997:Time Factors; D062065:Wastewater / Q000737:chemistry; D014874:Water Pollutants, Chemical / Q000032:analysis* / Q000737:chemistry
D000814:Aniline Compounds; D002374:Catalase; D003486:Cyanides; D004800:Enzymes, Immobilized; D005976:Glutaral; D062065:Wastewater; D014874:Water Pollutants, Chemical; C416807:polyaniline; C023650:aniline
PANI; catalase; cyanide; electrochemical impedance spectroscopy; inhibitory biosensor