A PCR detection method for testing Mycoplasma contamination of veterinary vaccines and biological products.
A rapid test method was developed for detecting mycoplasma contamination in veterinary biological products. The method reduces testing time by 2 weeks and shows comparable sensitivity to the current agar-based detection model. The primary goals for the development of the test were to reduce the testing time, incorporate a method that was easily adaptable across the veterinary biologics industry and reduce the subjective interpretation of results. We found that biological enrichment is necessary to maintain sensitivity of the detection method when compared to the standard culture-based test and that periodic sampling of enrichment cultures is essential to detect a wide variety of mycoplasma species that may be present as contaminants. The PCR detection method is comparable to the agar-based model and can reduce the overall testing time by up to 14 days.
Center for Veterinary Biologics, Veterinary Services, Animal and Plant Health Inspection Service, United States Department of Agriculture, Ames, IA, USA.
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Ingebritson
Center for Veterinary Biologics, Veterinary Services, Animal and Plant Health Inspection Service, United States Department of Agriculture, Ames, IA, USA.
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Gibbs
Center for Veterinary Biologics, Veterinary Services, Animal and Plant Health Inspection Service, United States Department of Agriculture, Ames, IA, USA.
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Tong
Center for Veterinary Biologics, Veterinary Services, Animal and Plant Health Inspection Service, United States Department of Agriculture, Ames, IA, USA.
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Srinivas
D001688:Biological Products; D004340:Drug Contamination; D009174:Mycoplasma / Q000254:growth & development / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014612:Vaccines; D019155:Veterinary Drugs
D001688:Biological Products; D014612:Vaccines; D019155:Veterinary Drugs
biological enrichment; mycoplasma; nucleic acid technology; touch-down PCR; veterinary biologics