Assay of mast cell mediators.
Mediator release from activated mast cells is a major initiator of the symptomology associated with allergic disorders such as anaphylaxis and asthma. Thus, methods to monitor the generation and release of such mediators have widespread applicability in studies designed to understand the processes regulating mast cell activation and for the identification of therapeutic approaches to block mast cell-driven disease. In this chapter, we discuss approaches used for the determination of mast cell degranulation, lipid-derived inflammatory mediator production, and cytokine/chemokine gene expression as well as cytokine release.
Krefting Research Centre, The Sahlgrenska Academy, University of Gothenburg, 424, Gothenburg, 405 30, Sweden, madeleine.radinger@gu.se.
Madeleine
M
Rådinger
Bettina M
BM
Jensen
Emily
E
Swindle
Alasdair M
AM
Gilfillan
D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology; D015550:Cell Degranulation; D016207:Cytokines / Q000096:biosynthesis / Q000378:metabolism; D018076:DNA, Complementary / Q000235:genetics; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D018836:Inflammation Mediators / Q000378:metabolism; D017997:Leukotriene C4 / Q000096:biosynthesis; D008407:Mast Cells / Q000166:cytology / Q000378:metabolism*; D051379:Mice; D016133:Polymerase Chain Reaction; D015230:Prostaglandin D2 / Q000096:biosynthesis
D016207:Cytokines; D018076:DNA, Complementary; D018836:Inflammation Mediators; D017997:Leukotriene C4; D015230:Prostaglandin D2