In vivo label-free quantification of liver microcirculation using dual-modality microscopy.
Microcirculation lesion is a common symptom of chronic liver diseases in the form of vasculature deformation and circulation alteration. In acute to chronic liver diseases such as biliary atresia, microcirculation lesion can have an early onset. Detection of microcirculation lesion is meaningful for studying the progression of liver disease. We have combined wide-field fluorescence microscopy and a laser speckle contrast technique to characterize hepatic microcirculation in vivo without labeling in a bile-duct ligation rat fibrosis model of biliary atresia. Through quantitative image analysis of four microcirculation parameters, we observed significant microcirculation lesion in the early to middle stages of fibrosis. This bimodal imaging method is useful to assess hepatic microcirculation lesion for the study of liver diseases.
BioSystems and Micromechanics IRG, Singapore-MIT Alliance for Research and Technology, 1 CREATE Way, #04-13/14 Enterprise Wing, 138602 Singapore, SingaporebInstitute of Bioengineering and Nanotechnology, 31 Biopolis Way, #04-01, 138669 Singapore, Singapor.
Jie
J
Yan
BioSystems and Micromechanics IRG, Singapore-MIT Alliance for Research and Technology, 1 CREATE Way, #04-13/14 Enterprise Wing, 138602 Singapore, SingaporedZhujiang Hospital of Southern Medical University, Department of Critical Care Medicine, 253 Gongye.
Yuzhan
Y
Kang
BioSystems and Micromechanics IRG, Singapore-MIT Alliance for Research and Technology, 1 CREATE Way, #04-13/14 Enterprise Wing, 138602 Singapore, SingaporebInstitute of Bioengineering and Nanotechnology, 31 Biopolis Way, #04-01, 138669 Singapore, Singapor.
Shuoyu
S
Xu
BioSystems and Micromechanics IRG, Singapore-MIT Alliance for Research and Technology, 1 CREATE Way, #04-13/14 Enterprise Wing, 138602 Singapore, Singapore.
Lee-Ling S
LL
Ong
BioSystems and Micromechanics IRG, Singapore-MIT Alliance for Research and Technology, 1 CREATE Way, #04-13/14 Enterprise Wing, 138602 Singapore, SingaporeeFujian Normal University, Institute of Laser and Optoelectronics Technology, Fuzhou 350007, China.
Shuangmu
S
Zhuo
Duke-NUS Graduate Medical School Singapore, 8 College Road, 169857 Singapore, Singapore.
Ralph M
RM
Bunte
National University of Singapore, Faculty of Engineering, Department of bioengineering, 9 Engineering Drive 1,117574 Singapore, Singapore.
Nanguang
N
Chen
BioSystems and Micromechanics IRG, Singapore-MIT Alliance for Research and Technology, 1 CREATE Way, #04-13/14 Enterprise Wing, 138602 Singapore, SingaporehMassachusetts Institute of Technology, Department of Mechanical Engineering, 77 Massachusetts Avenu.
H Harry
HH
Asada
BioSystems and Micromechanics IRG, Singapore-MIT Alliance for Research and Technology, 1 CREATE Way, #04-13/14 Enterprise Wing, 138602 Singapore, SingaporehMassachusetts Institute of Technology, Department of Mechanical Engineering, 77 Massachusetts Avenu.
Peter T C
PT
So
Dalhousie University, Victoria General Hospital, Department of Pathology, Halifax, Nova Scotia, B3H 2Y9 Canada.
Ian R
IR
Wanless
BioSystems and Micromechanics IRG, Singapore-MIT Alliance for Research and Technology, 1 CREATE Way, #04-13/14 Enterprise Wing, 138602 Singapore, SingaporebInstitute of Bioengineering and Nanotechnology, 31 Biopolis Way, #04-01, 138669 Singapore, Singapor.
Hanry
H
Yu
D000818:Animals; D001656:Biliary Atresia; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D008099:Liver / Q000098:blood supply*; D008103:Liver Cirrhosis / Q000473:pathology / Q000503:physiopathology; D008833:Microcirculation / Q000502:physiology*; D008853:Microscopy / Q000379:methods*; D012372:ROC Curve; D051381:Rats