G protein-coupled receptor signaling to Kir channels in Xenopus oocytes.
Kir3 (or GIRK) channels have been known for nearly three decades to be activated by direct interactions with the βγ subunits of heterotrimeric G (Gαβγ) proteins in a membrane-delimited manner. Gα also interacts with GIRK channels and since PTX-sensitive Gα subunits show higher affinity of interaction they confer signaling specificity to G Protein- Coupled Receptors (GPCRs) that normally couple to these G protein subunits. In heterologous systems, overexpression of non PTX-sensitive Gα subunits scavenges the available Gβγ and biases GIRK activation through GPCRs that couple to these Gα subunits. Moreover, all Kir channels rely on their direct interactions with the phospholipid PIP2 to maintain their activity. Thus, signals that activate phospholipase C (e.g. through Gq signaling) to hydrolyze PIP2 result in inhibition of Kir channel activity. In this review, we illustrate with experiments performed in Xenopus oocytes that Kir channels can be used efficiently as reporters of GPCR function through Gi, Gs or Gq signaling. The membrane-delimited nature of this expression system makes it highly efficient for constructing dose-response curves yielding highly reproducible apparent affinities of different ligands for each GPCR tested.
Candice
C
Hatcher-Solis
Miguel
M
Fribourg
Katerina
K
Spyridaki
Jason
J
Younkin
Amr
A
Ellaithy
Guoqing
G
Xiang
George
G
Liapakis
Javier
J
Gonzalez-Maeso
Hailin
H
Zhang
Meng
M
Cui
Department of Physiology and Biophysics, Medical College of Virginia Campus, Sanger Hall 3-038a, Virginia Commonwealth University, School of Medicine, 1101 E. Marshall Street, Richmond, VA 23298-0551, USA. delogothetis@vcu.edu.
Diomedes E
DE
Logothetis
D000818:Animals; D005260:Female; D009865:Oocytes / Q000378:metabolism*; D024661:Potassium Channels, Inwardly Rectifying / Q000378:metabolism*; D043562:Receptors, G-Protein-Coupled / Q000378:metabolism*; D015398:Signal Transduction; D014982:Xenopus laevis
D024661:Potassium Channels, Inwardly Rectifying; D043562:Receptors, G-Protein-Coupled