Targeted delivery of vaccine to dendritic cells by chitosan nanoparticles conjugated with a targeting peptide ligand selected by phage display technique.
The paper presents a novel dendritic cells (DC)-targeting peptide, TPAFRYS (TP) identified by phage display technique and conjugated to chitosan in order to develop an efficient DC-targeting vaccine delivery carrier. TP-conjugated chitosan nanoparticles (TPC-NPs) were prepared with ovalbumin (OVA) as a model vaccine by ionic gelation. Flow cytometry and immunocytochemistry studies demonstrated the higher targeting ability of TPC-NPs to DCs in compared to chitosan NPs. Moreover, TPC-NPs exhibited higher targeting specificity in DCs than macrophage and myoblasts. Furthermore, immunization of mice with OVA-loaded TPC-NPs enhanced OVA-specific serum IgG and IgG isotype antibodies production. Thus, DC-targeting strategy demonstrates a potential approach to enhance the effectiveness of vaccines.
Department of Agricultural Biotechnology and Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul, 151-921, Korea.
Su-Na
SN
Jung
Sang-Kee
SK
Kang
Guen-Hye
GH
Yeo
Hai-Ying
HY
Li
Tao
T
Jiang
Jae-Woon
JW
Nah
Jin-Duck
JD
Bok
Chong-Su
CS
Cho
Yun-Jaie
YJ
Choi
D000595:Amino Acid Sequence; D000818:Animals; D000918:Antibody Specificity; D001854:Bone Marrow Cells / Q000166:cytology; D002454:Cell Differentiation; D002460:Cell Line; D002469:Cell Separation; D061505:Cell Surface Display Techniques / Q000379:methods*; D048271:Chitosan / Q000737:chemistry*; D003713:Dendritic Cells / Q000166:cytology / Q000378:metabolism*; D016503:Drug Delivery Systems; D005260:Female; D008024:Ligands; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D053758:Nanoparticles / Q000737:chemistry*; D010047:Ovalbumin / Q000378:metabolism; D010455:Peptides / Q000737:chemistry*; D010641:Phenotype; D066244:Proton Magnetic Resonance Spectroscopy; D015203:Reproducibility of Results; D012995:Solubility; D014612:Vaccines / Q000276:immunology*; D014867:Water
D008024:Ligands; D010455:Peptides; D014612:Vaccines; D014867:Water; D010047:Ovalbumin; D048271:Chitosan
DC-targeting; chitosan; nanoparticles; phage display; vaccine delivery system