Effects of antibacterial peptide on cellular immunity in weaned piglets.
The aim of this study was to evaluate the effects of antibacterial peptide (ABP) sufficiency on cellular immune functions by determining the spleen cell cycle and apoptosis, peripheral blood T cell subsets, and T cell proliferation function in weaned piglets. A total of 90 piglets (Duroc × Landrace × Yorkshire) of both sexes were randomly allotted to 5 dietary treatments. Each treatment consisted of 3 replicates with 6 piglets per replicate. The dietary treatments consisted of the negative control (NC; basal diet), positive control (PC; basal diet supplemented with 400 mg/kg Astragalus polysaccharide), and ABP (basal diet mixed with 250, 500, and 1,000 mg/kg ABP). The experimental lasted for 28 d. Two piglets from each replicate were selected randomly for blood samples extraction from the jugular vein to obtain peripheral blood T cell subsets, and T cell proliferation function analysis was performed on d 32, 39, 46, and 53. Two piglets from each replicate were selected and euthanized to observe the spleen cell cycle and apoptosis on d 39 and 53. In ABP-sufficient piglets, the G0/G1 phase of the spleen cell cycle was much lower (P < 0.05) and the S and G2 + M phases and proliferation index (PI) were greater (P < 0.05) than in NC piglets. The percentage of apoptotic cells in the spleen significantly decreased under ABP sufficiency (P < 0.05). The proliferation function of peripheral blood T cells increased (P < 0.05) in ABP-sufficient piglets. Percentages of CD3 (+) and CD3 (+)CD4 (+) ratios (d 39, 46, and 53) and CD4 (+)CD8 (+) ratios (d 32, 39, 46, and 53) increased remarkably (P < 0.05) under ABP sufficiency compared with NC. These results suggest that ABP sufficiency could increase the T cell population and proliferation function of T cells and could induce decreased percentages of apoptotic cells. Overall, the cellular immune function was evidently improved in weaned piglets. We suggest optimal dosages of 500 mg/kg ABP for 4-wk addition and 1,000 mg/kg ABP for 2-wk addition.
Department of Veterinary Medicine, Sichuan Agricultural University, Yaan, Sichuan 625014, China.
Z H
ZH
Ren
Department of Veterinary Medicine, Sichuan Agricultural University, Yaan, Sichuan 625014, China.
W
W
Yuan
Department of Veterinary Medicine, Sichuan Agricultural University, Yaan, Sichuan 625014, China.
H D
HD
Deng
Department of Veterinary Medicine, Sichuan Agricultural University, Yaan, Sichuan 625014, China dengjl213@126.com.
J L
JL
Deng
Department of Veterinary Medicine, Sichuan Agricultural University, Yaan, Sichuan 625014, China.
Q X
QX
Dan
Department of Veterinary Medicine, Sichuan Agricultural University, Yaan, Sichuan 625014, China.
H T
HT
Jin
Department of Veterinary Medicine, Sichuan Agricultural University, Yaan, Sichuan 625014, China.
C L
CL
Tian
Department of Veterinary Medicine, Sichuan Agricultural University, Yaan, Sichuan 625014, China.
X
X
Peng
Department of Veterinary Medicine, Sichuan Agricultural University, Yaan, Sichuan 625014, China.
Z
Z
Liang
Department of Veterinary Medicine, Sichuan Agricultural University, Yaan, Sichuan 625014, China.
S
S
Gao
Qianxing Veterinary Pharmaceutical Co., Ltd, Chengdu, Sichuan 610000, China.
S H
SH
Xu
Rota Bioengineering Co., Ltd., Chengdu, Sichuan 610000, China.
G
G
Li
Rota Bioengineering Co., Ltd., Chengdu, Sichuan 610000, China.
Y
Y
Hu
D000818:Animals; D023181:Antimicrobial Cationic Peptides / Q000494:pharmacology*; D002453:Cell Cycle / Q000187:drug effects; D004032:Diet / Q000662:veterinary; D005260:Female; D007111:Immunity, Cellular / Q000187:drug effects / Q000276:immunology*; D008213:Lymphocyte Activation / Q000187:drug effects; D008297:Male; D011134:Polysaccharides; D013154:Spleen / Q000187:drug effects; D034421:Sus scrofa / Q000276:immunology* / Q000378:metabolism; D016176:T-Lymphocyte Subsets / Q000276:immunology
D023181:Antimicrobial Cationic Peptides; D011134:Polysaccharides
T cell subset; antimicrobial peptide; apoptosis; lymphocyte cycle; lymphocyte proliferation; weaned piglets