ARFGAP1 is dynamically associated with lipid droplets in hepatocytes.
The ARF GTPase Activating Protein 1 (ARFGAP1) associates mainly with the cytosolic side of Golgi cisternal membranes where it participates in the formation of both COPI and clathrin-coated vesicles. In this study, we show that ARFGAP1 associates transiently with lipid droplets upon addition of oleate in cultured cells. Also, that addition of cyclic AMP shifts ARFGAP1 from lipid droplets to the Golgi apparatus and that overexpression and knockdown of ARFGAP1 affect lipid droplet formation. Examination of human liver tissue reveals that ARFGAP1 is found associated with lipid droplets at steady state in some but not all hepatocytes.
The Research Institute of the McGill University Health Centre, McGill, Royal Victoria Hospital, 687 Pine Avenue West, Montreal, Quebec, Canada.
Joan
J
Gannon
The Centre for Cellular Imaging at the University of Gothenburg, Gothenburg, Sweden.
Julia
J
Fernandez-Rodriguez
The Research Institute of the McGill University Health Centre, McGill, Royal Victoria Hospital, 687 Pine Avenue West, Montreal, Quebec, Canada.
Hussam
H
Alamri
The Research Institute of the McGill University Health Centre, McGill, Royal Victoria Hospital, 687 Pine Avenue West, Montreal, Quebec, Canada.
Shi Bo
SB
Feng
The Research Institute of the McGill University Health Centre, McGill, Royal Victoria Hospital, 687 Pine Avenue West, Montreal, Quebec, Canada.
Fariba
F
Kalantari
The Research Institute of the McGill University Health Centre, McGill, Royal Victoria Hospital, 687 Pine Avenue West, Montreal, Quebec, Canada.
Sarita
S
Negi
The Research Institute of the McGill University Health Centre, McGill, Royal Victoria Hospital, 687 Pine Avenue West, Montreal, Quebec, Canada.
Amy H Y
AH
Wong
The Research Institute of the McGill University Health Centre, McGill, Royal Victoria Hospital, 687 Pine Avenue West, Montreal, Quebec, Canada.
Alexander
A
Mazur
The Department of Medical and Clinical Genetics, Institute of Biomedicine, University of Gothenburg, Gothenburg, Sweden.
Lennart
L
Asp
The Research Institute of the McGill University Health Centre, McGill, Royal Victoria Hospital, 687 Pine Avenue West, Montreal, Quebec, Canada.
Ali
A
Fazel
The Research Institute of the McGill University Health Centre, McGill, Royal Victoria Hospital, 687 Pine Avenue West, Montreal, Quebec, Canada.
Ayat
A
Salman
The Research Institute of the McGill University Health Centre, McGill, Royal Victoria Hospital, 687 Pine Avenue West, Montreal, Quebec, Canada.
Anthoula
A
Lazaris
The Research Institute of the McGill University Health Centre, McGill, Royal Victoria Hospital, 687 Pine Avenue West, Montreal, Quebec, Canada.
Peter
P
Metrakos
The Research Institute of the McGill University Health Centre, McGill, Royal Victoria Hospital, 687 Pine Avenue West, Montreal, Quebec, Canada.
John J M
JJ
Bergeron
The Research Institute of the McGill University Health Centre, McGill, Royal Victoria Hospital, 687 Pine Avenue West, Montreal, Quebec, Canada.
Tommy
T
Nilsson
D015415:Biomarkers / Q000378:metabolism; D045744:Cell Line, Tumor; D000242:Cyclic AMP / Q000494:pharmacology; D004721:Endoplasmic Reticulum / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D020690:GTPase-Activating Proteins / Q000378:metabolism*; D055785:Gene Knockdown Techniques; D006056:Golgi Apparatus / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D022781:Hepatocytes / Q000378:metabolism* / Q000648:ultrastructure; D006801:Humans; D066292:Lipid Droplets / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D019301:Oleic Acid / Q000494:pharmacology; D000070796:Perilipin-3; D021381:Protein Transport / Q000187:drug effects; D033921:Vesicular Transport Proteins / Q000378:metabolism
C503237:ARFGAP1 protein, human; D015415:Biomarkers; D020690:GTPase-Activating Proteins; C038196:PLIN3 protein, human; D000070796:Perilipin-3; D033921:Vesicular Transport Proteins; D019301:Oleic Acid; D000242:Cyclic AMP