SNP genotyping using KASPar assays.
In a separate chapter we describe a simple method for single nucleotide polymorphism (SNP) discovery using genomic reduction. Here we describe a scalable and cost-effective SNP genotyping method based on KBioscience's competitive allele-specific PCR amplification of target sequences and endpoint fluorescence genotyping (KASPar™) using a FRET capable plate reader or Fluidigm's dynamic array high-throughput platform.
Department of Plant and Microbial Biology, North Carolina State University, 600 Laureate Way, Kannapolis, 28081, NC, smsmit30@ncsu.edu.
Scott M
SM
Smith
Peter J
PJ
Maughan
D000483:Alleles; D017931:DNA Primers / Q000378:metabolism; D031541:Fluorescence Resonance Energy Transfer; D060005:Genotyping Techniques / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods*; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*
D017931:DNA Primers