Initial stem cell adhesion on porous silicon surface: molecular architecture of actin cytoskeleton and filopodial growth.
The way cells explore their surrounding extracellular matrix (ECM) during development and migration is mediated by lamellipodia at their leading edge, acting as an actual motor pulling the cell forward. Lamellipodia are the primary area within the cell of actin microfilaments (filopodia) formation. In this work, we report on the use of porous silicon (pSi) scaffolds to mimic the ECM of mesenchymal stem cells from the dental pulp (DPSC) and breast cancer (MCF-7) cells. Our atomic force microscopy (AFM), fluorescence microscopy, and scanning electron microscopy (SEM) results show that pSi promoted the appearance of lateral filopodia protruding from the DPSC cell body and not only in the lamellipodia area. The formation of elongated lateral actin filaments suggests that pores provided the necessary anchorage points for protrusion growth. Although MCF-7 cells displayed a lower presence of organized actin network on both pSi and nonporous silicon, pSi stimulated the formation of extended cell protrusions.
BioNano Laboratory EA 4203, Université Montpellier 1, Montpellier 34193, France.
Pierre-Yves
PY
Collart-Dutilleul
BioNano Laboratory EA 4203, Université Montpellier 1, Montpellier 34193, France.
Ivan
I
Panayotov
Matériaux Avancés Catalyse Santé, Institut Charles Gerhardt, UMR 5253 CNRS, Montpellier 34296, France.
Emilie
E
Secret
Matériaux Avancés Catalyse Santé, Institut Charles Gerhardt, UMR 5253 CNRS, Montpellier 34296, France.
Frédérique
F
Cunin
Laboratoire Charles Coulomb UMR 5221, Université Montpellier 2, Montpellier 34095, France ; Laboratoire Charles Coulomb UMR 5221, CNRS, Montpellier 34095, France.
Csilla
C
Gergely
BioNano Laboratory EA 4203, Université Montpellier 1, Montpellier 34193, France.
Frédéric
F
Cuisinier
Laboratoire Charles Coulomb UMR 5221, Université Montpellier 2, Montpellier 34095, France ; Laboratoire Charles Coulomb UMR 5221, CNRS, Montpellier 34095, France.
Marta
M
Martin
Cell adhesion; Mesenchymal stem cells; Porosity; Tissue engineering