Direct cytosolic delivery of siRNA using nanoparticle-stabilized nanocapsules.
The use of nanoparticle-stabilized nanocapsules (NPSCs) for the direct cytosolic delivery of siRNA is reported. In this approach, siRNA is complexed with cationic arginine-functionalized gold nanoparticles by electrostatic interactions, with the resulting ensemble self-assembled onto the surface of fatty acid nanodroplets to form a NPSC/siRNA nanocomplex. The complex rapidly delivers siRNA into the cytosol through membrane fusion, a mechanism supported by cellular uptake studies. Using destabilized green fluorescent protein (deGFP) as a target, 90% knockdown was observed in HEK293 cells. Moreover, the delivery of siRNA targeting polo-like kinase 1 (siPLK1) efficiently silenced PLK1 expression in cancer cells with concomitant cytotoxicity.
Department of Chemistry, University of Massachusetts Amherst, 710 North Pleasant Street, Amherst, MA 01003 (USA).
Ying
Y
Jiang
Rui
R
Tang
Bradley
B
Duncan
Ziwen
Z
Jiang
Bo
B
Yan
Rubul
R
Mout
Vincent M
VM
Rotello
D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles; D010455:Peptides / Q000737:chemistry*; D034741:RNA, Small Interfering / Q000008:administration & dosage / Q000737:chemistry*
D010455:Peptides; D034741:RNA, Small Interfering
RNA; antitumor agents; cancer; gold; nanoparticles