miR‑107 promotes the erythroid differentiation of leukemia cells via the downregulation of Cacna2d1.
microRNAs (miRNAs) have been reported to be involved in various human diseases. They may have uses in diagnosis and as therapeutic targets, thus the discovery of novel miRNAs has the potential to provide clinical tools or shed light on novel mechanisms. In the current study, miR‑107 was revealed to be downregulated in chronic myeloid leukemia cells. Overexpression of miR‑107 in K562 and KCL‑22 chronic myeloid leukemia cells promotes erythroid differentiation, while having no effect on cell proliferation. Further bioinformatics predicted that one target of miR‑107 may be Cacna2d1, a calcium channel protein. A luciferase reporter assay and quantitative polymerase chain reaction were utilized to confirm that Cacna2d1 is a target molecule of miR-107. The effect of miR‑107 on K562 and KCL‑22 cells was mediated through the downregulation of Cacna2d1, as rescued expression of Cacna2d1 reversed the effects of miR‑107. In summary, the current study identified a novel miRNA that is involved in chronic myeloid leukemia cell erythroid differentiation and the associated mechanisms, making it a potential therapeutic target in the treatment of chronic myeloid leukemia.
Department of Clinical Laboratory, Chongqing Medical University, Chongqing 400016, P.R. China.
Jie
J
Ruan
Department of Clinical Laboratory, Chongqing Medical University, Chongqing 400016, P.R. China.
Xinguang
X
Liu
Institute of Aging Research, Guangdong Medical College, Dongguan, Guandong 523808, P.R. China.
Xingdong
X
Xiong
Department of Clinical Laboratory, Chongqing Medical University, Chongqing 400016, P.R. China.
Chunlong
C
Zhang
Institute of Aging Research, Guangdong Medical College, Dongguan, Guandong 523808, P.R. China.
Jiangbin
J
Li
Institute of Aging Research, Guangdong Medical College, Dongguan, Guandong 523808, P.R. China.
Huiling
H
Zheng
Institute of Laboratory Medicine, Guangdong Medical College, Dongguan, Guandong 523808, P.R. China.
Chirong
C
Huang
Department of Clinical Laboratory, Chongqing Medical University, Chongqing 400016, P.R. China.
Qiong
Q
Shi
Department of Clinical Laboratory, Chongqing Medical University, Chongqing 400016, P.R. China.
Yaguang
Y
Weng
D020413:3' Untranslated Regions; D015703:Antigens, CD / Q000378:metabolism; D001483:Base Sequence; D015220:Calcium Channels / Q000235:genetics / Q000378:metabolism*; D002454:Cell Differentiation; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015536:Down-Regulation; D041905:Erythroid Cells / Q000166:cytology; D016044:Fusion Proteins, bcr-abl / Q000378:metabolism; D006801:Humans; D020014:K562 Cells; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000378:metabolism / Q000473:pathology; D035683:MicroRNAs / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D009841:Oligonucleotides / Q000378:metabolism; D011990:Receptors, Transferrin / Q000378:metabolism; D016415:Sequence Alignment; D014162:Transfection
D020413:3' Untranslated Regions; D015703:Antigens, CD; C425903:CACNA2D1 protein, human; C068126:CD71 antigen; D015220:Calcium Channels; C549045:MIRN107 microRNA, human; D035683:MicroRNAs; D009841:Oligonucleotides; D011990:Receptors, Transferrin; D016044:Fusion Proteins, bcr-abl