An insight to the conserved water mediated dynamics of catalytic His88 and its recognition to thyroxin and RBP binding residues in human transthyretin.
Human transthyretin (hTTR) is a multifunctional protein involved in several amyloidogenic diseases. Besides transportation of thyroxin and vitamin-A, its role towards the catalysis of apolipoprotein-A1 and Aβ-peptide are also drawing interest. The role of water molecules in the catalytic mechanism is still unknown. Extensive analyses of 14 high-resolution X-ray structures of human transthyretin and MD simulation studies have revealed the presence of eight conserved hydrophilic centres near its catalytic zone which may be indispensable for the function, dynamics and stability of the protein. Three water molecules (W1, W2 and W3) form a cluster and play an important role in the recognition of the catalytic and RBP-binding residues. They also induce the reorganisation of the His88 for coupling with other catalytic residues (His90, Glu92). Another water molecule (W5) participate in inter-monomer recognition between the catalytic and thyroxin binding sites. The rest four water molecules (W6, W*, W(#) and W(†)) form a distorted tetrahedral cluster and impart stability to the catalytic core of hTTR. The conserved water mediated recognition dynamics of the different functional sites may provide some rational clues towards the understanding of the activity and mechanism of hTTR.
a Department of Chemistry , National Institute of Technology-Durgapur , Durgapur 713209 , West Bengal , India.
Avik
A
Banerjee
Bishnu P
BP
Mukhopadhyay
D001054:Apolipoproteins A / Q000737:chemistry; D001665:Binding Sites; D002384:Catalysis; D018360:Crystallography, X-Ray; D006639:Histidine / Q000737:chemistry* / Q000235:genetics; D006801:Humans; D006860:Hydrogen Bonding; D056004:Molecular Dynamics Simulation; D011228:Prealbumin / Q000737:chemistry* / Q000378:metabolism; D017434:Protein Structure, Tertiary; D013974:Thyroxine / Q000737:chemistry*; D014801:Vitamin A / Q000737:chemistry / Q000378:metabolism; D014867:Water / Q000737:chemistry*
D001054:Apolipoproteins A; D011228:Prealbumin; D014867:Water; D014801:Vitamin A; D006639:Histidine; D013974:Thyroxine
conserved water; human-transthyretin; molecular dynamics; proteolysis; retinol binding protein