Phylogenetic analysis of a G6P[5] bovine rotavirus strain isolated in a neonatal diarrhea outbreak in a beef cattle herd vaccinated with G6P[1] and G10P[11] genotypes.
The aim of this study was to perform the molecular characterization of the eleven genes of a G6P[5] bovine group A rotavirus (RVA) strain detected in a diarrhea outbreak from a vaccinated beef cattle herd. The outbreak affected 80 % of calves between 15-30 days old. RVA was identified by RT-PCR in 12 (70.6 %) out of 17 diarrheic fecal samples evaluated. The rotavirus wild-type strain had the genotype constellation G6(IV)-P[5](IX)-I2c-R2-C2-M2-A3-N2-T6-E2e-H3a. This study confirms the importance of homotypic immunity against the bovine RVA P[5] genotype in neonatal diarrhea in cattle herds that are regularly vaccinated against rotaviruses.
Laboratory of Animal Virology, Department of Veterinary Preventive Medicine, Universidade Estadual de Londrina, Celso Garcia Cid Road, PR455 Km 380, P.O. Box 10.011, Londrina, PR, 86057-970, Brazil.
Thais Neris
TN
da Silva Medeiros
Elis
E
Lorenzetti
Alice Fernandes
AF
Alfieri
Amauri Alcindo
AA
Alfieri
D000818:Animals; D000956:Antigens, Viral / Q000235:genetics; D036022:Capsid Proteins / Q000235:genetics; D002417:Cattle; D002418:Cattle Diseases / Q000821:virology*; D003967:Diarrhea / Q000517:prevention & control / Q000662:veterinary* / Q000821:virology; D004196:Disease Outbreaks / Q000517:prevention & control / Q000662:veterinary*; D005243:Feces / Q000821:virology; D016679:Genome, Viral; D010802:Phylogeny; D012401:Rotavirus / Q000145:classification* / Q000235:genetics; D012400:Rotavirus Infections / Q000453:epidemiology* / Q000662:veterinary* / Q000821:virology; D014611:Vaccination
D000956:Antigens, Viral; D036022:Capsid Proteins; C060339:VP4 protein, Rotavirus; C050779:VP7 protein, Rotavirus