Lab-in-a-syringe using gold nanoparticles for rapid immunosensing of protein biomarkers.
We have developed a paper and gold nanoparticle (AuNP)-based lab-in-a-syringe (LIS) for immunosensing of biomarkers. This simple diagnostic device features simultaneous sampling and vertical-flow operation, which means that unlike typical immunosensors, it does not suffer from any delay between sampling and detection. It can handle large-volume, low-concentration samples for analysis in diverse applications (e.g. biomedical, environmental, food, etc.). Furthermore, its operating range for sample concentration can be tuned by simply changing the volume of the syringed sample, which enables on-demand limits of detection (LOD). The LIS contains two nitrocellulose pads: the conjugate pad (which captures the analyte) and the detection pad (which signals the presence of the captured analyte) both embedded into reusable plastic cartridges. We demonstrated its efficiency in detecting human IgG (HIgG) (LOD: 1.0 ng mL(-1)) and prostate-specific antigen (PSA) (spiked urine samples; LOD: 1.9 ng mL(-1)). In the field, the LIS can be used for complete on-site analysis or to obtain partially analyzed samples (AuNPs with captured analyte) for subsequent detailed testing in specialized laboratories.
ICN2 - Nanobioelectronics & Biosensors Group, Institut Catala de Nanociencia i Nanotecnologia, Campus UAB, 08193 Bellaterra, Barcelona, Spain. arben.merkoci@icn.cat.
Gisele Elias
GE
Nunes Pauli
Alfredo
A
de la Escosura-Muñiz
Claudio
C
Parolo
Ivan
I
Helmuth Bechtold
Arben
A
Merkoçi
D000906:Antibodies / Q000737:chemistry / Q000276:immunology; D055910:Antibodies, Immobilized / Q000737:chemistry / Q000276:immunology; D015415:Biomarkers / Q000032:analysis; D006046:Gold / Q000737:chemistry*; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation / Q000379:methods*; D007074:Immunoglobulin G / Q000032:analysis*; D056656:Lab-On-A-Chip Devices; D057230:Limit of Detection; D008297:Male; D053768:Metal Nanoparticles / Q000737:chemistry*; D017430:Prostate-Specific Antigen / Q000032:analysis* / Q000652:urine; D013594:Syringes
D000906:Antibodies; D055910:Antibodies, Immobilized; D015415:Biomarkers; D007074:Immunoglobulin G; D006046:Gold; D017430:Prostate-Specific Antigen