Impacts of CLA and dietary concentrate proportion on blood metabolite concentration and proliferation of peripheral blood mononuclear cells of periparturient dairy cows.
The study aimed to examine effects of supplemented CLA to periparturient dairy cows receiving different concentrate proportions antepartum (a.p.) to investigate CLA effects on metabolism and immune function. Compared with adapted feeding, high-concentrate diet a.p. should induce a ketogenic metabolic situation postpartum (p.p.) to better understand how CLA works. A total of 64 pregnant German Holstein cows had ad libitum access to partial mixed rations based on concentrate and roughage 3 weeks before calving until day 60 p.p. A.p., cows received 100 g/day control fat (CON) or a CLA supplement, either in a low-concentrate (20%, CON-20, CLA-20) or high-concentrate diet (60%, CON-60, CLA-60). P.p., concentrate proportion was adjusted to 50% while fat supplementation continued. After day 32 p.p., half of the animals of CLA-groups changed to CON supplementation (CLA-20-CON, CLA-60-CON). A ketogenic metabolic state p.p. was not achieved and respective impacts of CLA could not be examined. Blood samples for isolation of peripheral blood mononuclear cells (PBMC) were collected on day -21, 7, 28 and 56 relative to calving. Blood chemistry samples were taken over the entire experimental period. Mitogen-stimulated proliferation of PBMC remained unaffected. Besides serum concentrations of triglycerides, total bilirubin, total protein, albumin and IGF-1, clinical-chemical serum characteristics remained uninfluenced by treatments. No post-supplementation effect could be observed. Measured blood metabolites and mitogen-stimulated proliferation of PBMC indicate that all groups had an increased metabolic stress around calving, whereby group CLA-20 was affected more severely. Overall, supplemented CLA did not positively affect metabolism or immune function of periparturient dairy cows. However, feeding CLA in a low-concentrate diet a.p. seems to increase liver stress around calving via reduced DMI.
1Institute of Animal Nutrition,Friedrich-Loeffler-Institute (FLI),Federal Research Institute for Animal Health,38116 Braunschweig,Germany.
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Petzold
1Institute of Animal Nutrition,Friedrich-Loeffler-Institute (FLI),Federal Research Institute for Animal Health,38116 Braunschweig,Germany.
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Meyer
1Institute of Animal Nutrition,Friedrich-Loeffler-Institute (FLI),Federal Research Institute for Animal Health,38116 Braunschweig,Germany.
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Kersten
2Institute of Agricultural and Nutritional Sciences,Biometrics and Informatics in Agriculture Group,Martin-Luther University Halle-Wittenberg,06120 Halle (Saale),Germany.
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Spilke
3Department of Physiology,University of Veterinary Medicine,30173 Hannover,Germany.
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Breves
1Institute of Animal Nutrition,Friedrich-Loeffler-Institute (FLI),Federal Research Institute for Animal Health,38116 Braunschweig,Germany.
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Dänicke
D000824:Animal Nutritional Physiological Phenomena / Q000502:physiology*; D000818:Animals; D001663:Bilirubin / Q000097:blood; D001798:Blood Proteins / Q000032:analysis; D002417:Cattle / Q000502:physiology*; D049109:Cell Proliferation / Q000187:drug effects*; D004032:Diet / Q000662:veterinary*; D019587:Dietary Supplements; D005260:Female; D007334:Insulin-Like Growth Factor I / Q000378:metabolism; D007963:Leukocytes, Mononuclear / Q000187:drug effects*; D044243:Linoleic Acids, Conjugated / Q000494:pharmacology*; D008099:Liver / Q000187:drug effects; D015233:Models, Statistical; D049590:Postpartum Period / Q000187:drug effects; D011247:Pregnancy; D012709:Serum Albumin / Q000032:analysis; D013778:Tetrazolium Salts; D013844:Thiazoles; D013997:Time Factors; D014280:Triglycerides / Q000097:blood; D014343:Trypan Blue
D001798:Blood Proteins; D044243:Linoleic Acids, Conjugated; D012709:Serum Albumin; D013778:Tetrazolium Salts; D013844:Thiazoles; D014280:Triglycerides; C496197:trans-10,cis-12-conjugated linoleic acid; D007334:Insulin-Like Growth Factor I; C022616:thiazolyl blue; D014343:Trypan Blue; D001663:Bilirubin
peripheral blood mononuclear cells