Highly ordered nanoporous carbon films with tunable pore diameters and their excellent sensing properties.
Ordered porous carbon films with tunable pore diameters, immobilized with glucose oxidase (GOD) have been fabricated and employed for the construction of a biosensor for glucose molecules. The as-prepared porous films have large specific surface areas and highly ordered porous structure with uniform pore sizes, which are critical for the immobilization of large amounts of GOD and support the promotion of heterogeneous electron transfer. The developed biosensors give enough room for the encapsulation of a high amount of GOD molecules and show excellent biosensing performance with a linear response to glucose concentration ranging from 0.5 to 9 mM and a detection limit of 1.5 μM. It is also demonstrated that the sensitivity of the biosensor can be easily tuned by modulating the pore size of carbon film as it dictates the amount of immobilization of GOD in the porous channels. The fabricated carbon-film-based biosensor has a good stability and a high reproducibility, which opens the gateway for the commercialization of this excellent technology.
International Centre for Materials Nanoarchitectonics, WPI Research Centre, National Institute for Materials Science, 1-1 Namiki, Tsukuba 305-0044 (Japan).
Lichao
L
Jia
Geoffrey
G
Lawrence
V V
VV
Balasubramanian
Goeun
G
Choi
Jin-Ho
JH
Choy
Aboubakr M
AM
Abdullah
Ahmed
A
Elzatahry
Katsuhiko
K
Ariga
Ajayan
A
Vinu
D001230:Aspergillus / Q000201:enzymology; D015374:Biosensing Techniques / Q000379:methods*; D002244:Carbon / Q000737:chemistry*; D004800:Enzymes, Immobilized / Q000378:metabolism; D005947:Glucose / Q000032:analysis* / Q000378:metabolism; D005949:Glucose Oxidase / Q000378:metabolism; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure; D016062:Porosity; D015203:Reproducibility of Results
D004800:Enzymes, Immobilized; D002244:Carbon; D005949:Glucose Oxidase; D005947:Glucose
biosensors; carbon; nanostructures; porous materials; thin films