Interaction of Omega, Sigma, and Theta glutathione transferases with p38b mitogen-activated protein kinase from the fruit fly, Drosophila melanogaster.
Glutathione S-transferases (GSTs) are a diverse family of phase II detoxification enzymes found in almost all organisms. Besides playing a major role in the detoxification of xenobiotic and toxic compounds, GSTs are also involved in the regulation of mitogen activated protein (MAP) kinase signal transduction by interaction with proteins in the pathway. An in vitro study was performed for Theta, Omega, Sigma GSTs and their interaction with MAP kinase p38b protein from the fruit fly Drosophila melanogaster Meigen (Diptera: Drosophilidae). The study included the effects of all five Omega class GSTs (DmGSTO1, DmGSTO2a, DmGSTO2b, DmGSTO3, DmGSTO4), all five Theta class GSTs (DmGSTT1, DmGSTT2, DmGSTT3a, DmGSTT3b, DmGSTT4), and one Sigma class glutathione transferase on the activity of Drosophila p38b, including the reciprocal effect of this kinase protein on glutathione transferase activity. It was found that DmGSTT2, DmGSTT3b, DmGSTO1, and DmGSTO3 activated p38b significantly. Substrate specificities of GSTs were also altered after co-incubation with p38b. Although p38b activated DmGSTO1, DmGSTO2a, and DmGSTT2, it inhibited DmGSTT3b and DmGSTO3 activity toward xenobiotic and physiological substrates tested. These results suggest a novel link between Omega and Theta GSTs with the p38b MAP kinase pathway.
Research Institute for Health Sciences (RIHES), Chiang Mai University, P.O.BOX 80 CMU, Chiang Mai, Thailand 50200 jwongtrakul@yahoo.com.
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Wongtrakul
Research Institute for Health Sciences (RIHES), Chiang Mai University, P.O.BOX 80 CMU, Chiang Mai, Thailand 50200 kanokwanjan@yahoo.com.
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Janphen
Institute of Molecular Biosciences, Mahidol University, Nakhon Pathom, Thailand 73170 biotbug@yahoo.com.
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Saisawang
Institute of Molecular Biosciences, Mahidol University, Nakhon Pathom, Thailand 73170 albertketterman@yahoo.com.
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Ketterman
D000818:Animals; D004331:Drosophila melanogaster / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology*; D005982:Glutathione Transferase / Q000145:classification* / Q000235:genetics / Q000378:metabolism; D048290:Mitogen-Activated Protein Kinase 11 / Q000235:genetics / Q000378:metabolism*; D013379:Substrate Specificity; D059467:Transcriptome
D005982:Glutathione Transferase; D048290:Mitogen-Activated Protein Kinase 11