Adjuvant effects of recombinant giant panda (Ailuropoda melanoleuca) IL-18 on the canine distemper disease vaccine in mice.
Canine distemper virus (CDV) is a morbillivirus known to cause morbidity and mortality in a broad range of animals. Giant pandas (Ailuropoda melanoleuca), especially captive ones, are susceptible to natural infection with CDV. Interleukin-18 (IL-18) is a powerful adjuvant molecule that can enhance the development of antigen-specific immunity and vaccine efficacy. In this study, a giant panda IL-18 gene eukaryotic expression plasmid (pcAmIL-18) was constructed. Female BALB/c mice were muscularly inoculated with the plasmids pcAmIL-18, pcDNA3.1 and PBS, respectively. They were subsequently injected with an attenuated CDV vaccine for dogs, and the induced humoral and cellular responses were evaluated. The results showed that pcAmIL-18 remarkably improved the level of specific antibody, IFN-γ and IL-2 in mice sera, the T lymphocyte proliferation index and the percentage of CD4(+) and CD8(+) cells. These data indicated that pcAmIL-18 is a potential adjuvant that promotes specific immunity.
College of Life Sciences, Sichuan University, Key Laboratory for Bio-Resources and Eco-Environment of the Ministry of Education, Sichuan Key Laboratory of Molecular Biology and Biotechnology, Chengdu 610064, PR China.
Yue
Y
Yan
Lili
L
Niu
Jiabo
J
Deng
Qiang
Q
Wang
Jianqiu
J
Yu
Yizheng
Y
Zhang
Jianxi
J
Wang
Jiao
J
Chen
Changhe
C
Wei
Xuemei
X
Tan
D000276:Adjuvants, Immunologic; D000818:Animals; D004216:Distemper / Q000517:prevention & control*; D004285:Dogs; D006367:HeLa Cells; D006801:Humans; D020382:Interleukin-18 / Q000494:pharmacology*; D051379:Mice; D001503:Ursidae; D014765:Viral Vaccines / Q000276:immunology*
D000276:Adjuvants, Immunologic; D020382:Interleukin-18; D014765:Viral Vaccines