Transfer of silica-coated magnetic (Fe3O4) nanoparticles through food: a molecular and morphological study in zebrafish.
The increasing use of magnetic iron oxide nanoparticles (NPs) in biomedical applications has prompted extensive investigation of their interactions with biological systems also through animal models. A variety of toxic effects have been detected in NP-exposed fish and fish embryos, including oxidative stress and associated changes, such as lipid oxidation, apoptosis, and gene expression alterations. The main exposure route for fish is through food and the food web. This study was devised to investigate the effects of silica-coated NP administration through food in zebrafish (ZF, Danio rerio). Silica-coated magnetic NPs were administered to ZF through feed (zooplankton) from day 1 to 15 posthatching (ph). Larvae were examined 6 and 15 days ph and adults 3 and 6 months ph. A multidisciplinary approach, including morphometric examination; light, transmission electron, and confocal microscopy; inductively coupled plasma emission spectrometry; and real-time polymerase chain reaction, was applied to detect NP accumulation, structural and ultrastructural damage, and activation of detoxification processes in larvae and adults. Our findings document that the silica-coated NPs: (1) do not induce toxicity in ZF, (2) are excreted through feces, and (3) do not activate detoxification processes or promote tissue/cell injury.
1 Dipartimento di Scienze della Vita e dell'Ambiente, Università Politecnica delle Marche , Ancona, Italy .
Chiara Carla
CC
Piccinetti
Costanza
C
Montis
Massimo
M
Bonini
Rosaria
R
Laurà
Maria Cristina
MC
Guerrera
Giuseppe
G
Radaelli
Fabio
F
Vianello
Veronica
V
Santinelli
Francesca
F
Maradonna
Valentina
V
Nozzi
Andrea
A
Miccoli
Ike
I
Olivotto
D000818:Animals; D001837:Body Weights and Measures; D020387:Food Chain; D007814:Larva / Q000378:metabolism; D058185:Magnetite Nanoparticles / Q000008:administration & dosage / Q000032:analysis* / Q000633:toxicity; D018613:Microscopy, Confocal; D046529:Microscopy, Electron, Transmission; D060888:Real-Time Polymerase Chain Reaction; D012822:Silicon Dioxide / Q000008:administration & dosage / Q000032:analysis; D013054:Spectrophotometry, Atomic; D015027:Zebrafish / Q000378:metabolism*; D015048:Zooplankton / Q000737:chemistry*
D058185:Magnetite Nanoparticles; D012822:Silicon Dioxide