Comparability of higher order structure in proteins: chemometric analysis of second-derivative amide I Fourier transform infrared spectra.
Comparing higher order structure (HOS) in therapeutic proteins is a significant challenge. Previously, we showed that changes in solution conditions produced detectable changes in the second-derivative amide I Fourier transform infrared (FTIR) spectra for a variety of model proteins. Those comparisons utilized vector-based approaches, such as spectral overlap and spectral correlation coefficients to quantify differences between spectra. In this study, chemometric analyses of the same data were performed, to classify samples into different groups based on the solution conditions received. The solution conditions were composed of various combinations of temperature, pH, and salt types. At first, principal component analysis (PCA) was used to visually demonstrate that FTIR spectra respond to changes in solution conditions, which, presumably indicates variations in HOS. This observed when samples from the same solution condition form clusters within a PCA score plot. The second approach, called soft independent modeling of class analogy (SIMCA), was conducted to account for the within-class experimental error for the lysozyme spectra. The DModX values, indicative of the distance of each spectra to their respective class models, was found to be a more sensitive quantitative indicator of changes in HOS, when compared with the modified area of overlap algorithm. The SIMCA approach provides a metric to determine whether new observations do, or do not belong to a particular class or group. Thus, SIMCA is the recommended approach when multiple samples from each condition are available.
Department of Statistical Sciences, Projects Clinical Platform, R&D, GlaxoSmithKline, King of Prussia, Pennsylvania, 19406.
Gregory
G
Stockdale
Brian M
BM
Murphy
Jennifer
J
D'Antonio
Mark Cornell
MC
Manning
Wasfi
W
Al-Azzam
D000465:Algorithms; D053000:Analytic Sample Preparation Methods; D000818:Animals; D002626:Chemistry, Pharmaceutical / Q000379:methods*; D002645:Chickens; D019295:Computational Biology; D006736:Horses; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007074:Immunoglobulin G / Q000737:chemistry*; D008958:Models, Molecular; D009113:Muramidase / Q000737:chemistry*; D009211:Myoglobin / Q000737:chemistry*; D025341:Principal Component Analysis; D011487:Protein Conformation; D058767:Protein Unfolding; D017550:Spectroscopy, Fourier Transform Infrared; D013696:Temperature
D007074:Immunoglobulin G; D009211:Myoglobin; C421864:hen egg lysozyme; D009113:Muramidase
FTIR; biopharmaceuticals characterization; chemometrics; protein structure; proteins; spectroscopy; stability