One-step click engineering considerably ameliorates the practicality of an unqualified rhodamine probe.
This study describes the exploitation of click chemistry in the one-step molecular engineering of an unqualified rhodamine probe, leading to its considerable functional enhancement in terms of water solubility, ion selectivity, and usefulness in detecting biological and environmental samples. A dipropargyl rhodamine dye previously identified as an unselective and poorly water-soluble mercury(II) probe was used to couple with an azido polyethylene glycol (PEG) by the Cu(I)-catalyzed azide-alkyne 1,3-dipolar cycloaddition click reaction in almost quantitative yield. The simple click-engineered rhodamine probe shows, remarkably, better water solubility and mercury(II) selectivity comparing to the raw counterpart, and can be used to sensitively image mercury ions internalized by live cells and to accurately quantify the ion spiked in river water specimens. This study provides insights into the simple functional improvement of unqualified molecular dye probes via the efficient "click engineering".
Key Laboratory for Advanced Materials & Institute of Fine Chemicals, East China University of Science and Technology , 130 Meilong Road, Shanghai 200237, PR China.
Kai-Bin
KB
Li
Huan
H
Wang
Yi
Y
Zang
Xiao-Peng
XP
He
Jia
J
Li
Guo-Rong
GR
Chen
He
H
Tian
D000480:Alkynes / Q000737:chemistry; D001386:Azides / Q000737:chemistry; D002384:Catalysis; D057930:Click Chemistry; D003300:Copper / Q000737:chemistry*; D007477:Ions / Q000737:chemistry; D008628:Mercury / Q000302:isolation & purification*; D011092:Polyethylene Glycols / Q000737:chemistry; D012235:Rhodamines / Q000737:chemistry*; D014873:Water Pollutants / Q000737:chemistry / Q000302:isolation & purification*
D000480:Alkynes; D001386:Azides; D007477:Ions; D012235:Rhodamines; D014873:Water Pollutants; D011092:Polyethylene Glycols; D003300:Copper; D008628:Mercury
PEG; cell imaging; click chemistry; probe; rhodamine; triazole