Oral vaccination of mice with Tremella fuciformis yeast-like conidium cells expressing HBsAg.
Tremella fuciformis yeast-like conidium (YLC) cells were transformed by co-cultivation with Agrobacterium cells harboring the hepatitis B surface antigen (HBsAg) gene construct under the control of the CaMV35S promoter. Integration of HBsAg DNA into the YLC genome was confirmed by PCR and dot-blot hybridization. Immunoblotting verified expression of the recombinant protein. Oral administration of YLC cells expressing HBsAg in mice significantly increased anti-HBsAg antibody titer levels using a double prime-boost strategy that combined parenteral and oral HBsAg boosters.
Department of Biotechnology, Catholic University of Daegu, Gyeongsan, 712-702, Korea.
Dong-Il
DI
Shin
Kyu-Seon
KS
Song
Hee-Sung
HS
Park
D000284:Administration, Oral; D000818:Animals; D001487:Basidiomycota / Q000235:genetics*; D004337:Drug Carriers / Q000008:administration & dosage*; D005822:Genetic Vectors; D006510:Hepatitis B Antibodies / Q000097:blood; D006514:Hepatitis B Surface Antigens / Q000235:genetics / Q000276:immunology*; D017325:Hepatitis B Vaccines / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D051379:Mice; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology; D013172:Spores, Fungal / Q000235:genetics; D014611:Vaccination / Q000379:methods*; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology
D004337:Drug Carriers; D006510:Hepatitis B Antibodies; D006514:Hepatitis B Surface Antigens; D017325:Hepatitis B Vaccines; D011994:Recombinant Proteins; D014614:Vaccines, Synthetic