Resveratrol exerts an anti-apoptotic effect on human bronchial epithelial cells undergoing cigarette smoke exposure.
Cigarette smoke can cause endoplasmic reticulum stress and induce apoptosis, both of which are important pathogenic factors contributing to chronic obstructive pulmonary disease. The aim of the present study was to produce a cigarette smoke extract (CSE)‑induced apoptosis human bronchial epithelial cell (HBEpC) model, to investigate the protective effects of resveratrol (RES). The role of oxygen‑regulated protein 150 (ORP150) in the RES‑induced activation of Sirtuin 1 (SIRT1) was additionally studied. Cultured HBEpCs were initially treated with CSE to induce apoptosis, followed by an incubation either with or without RES. Numerous techniques were used to evaluate the outcomes of the present study, including cell counting kit‑8 assay, quantitative polymerase chain reaction, western blotting, Hoechst 33342 staining and AnnexinV‑PI flow cytometry apoptosis analyses, and gene knockdown. It was identified that 24 h 2% CSE incubation induced apoptosis in HBEpC, accompanied by an overexpression of the apoptosis molecular markers CCAAT‑enhancer‑binding protein homologous protein, caspase 4 and caspase 3. Pre‑treatment of the cells with RES markedly alleviated the severity of apoptosis, as confirmed by apoptosis analyses and the expression levels of the apoptosis molecular markers. SIRT1 was shown to be overexpressed following RES treatment. However, following the gene knockdown of ORP150, the anti‑apoptotic effects of RES were significantly attenuated. The results of the present study demonstrate that RES may have a protective effect against CSE‑induced apoptosis, and a molecular pathway involving SIRT1 and ORP150 may be associated with the anti‑apoptotic functions of RES in HBEpC.
Department of Respiratory Medicine, Xiangya Hospital, Central South University, Changsha, Hunan 410008, P.R. China.
Li
L
Zhang
Department of Respiratory Medicine, Xiangya Hospital, Central South University, Changsha, Hunan 410008, P.R. China.
Xialing
X
Guo
Department of Respiratory Medicine, Xiangya Hospital, Central South University, Changsha, Hunan 410008, P.R. China.
Wang
W
Xie
Department of Respiratory Medicine, Xiangya Hospital, Central South University, Changsha, Hunan 410008, P.R. China.
Yuping
Y
Li
Department of Respiratory Medicine, Xiangya Hospital, Central South University, Changsha, Hunan 410008, P.R. China.
Miao
M
Ma
Department of Critical Emergency Medicine, The Second Xiangya Hospital, Central South University, Changsha, Hunan 410007, P.R. China.
Ting
T
Yuan
Department of Respiratory Medicine, Xiangya Hospital, Central South University, Changsha, Hunan 410008, P.R. China.
Bailing
B
Luo
D017209:Apoptosis / Q000187:drug effects* / Q000235:genetics; D002460:Cell Line; D004847:Epithelial Cells / Q000187:drug effects* / Q000378:metabolism*; D055786:Gene Knockout Techniques; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics; D006801:Humans; D020011:Protective Agents / Q000494:pharmacology; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics; D020545:Respiratory Mucosa / Q000166:cytology*; D000077185:Resveratrol; D056564:Sirtuin 1 / Q000235:genetics; D012906:Smoke / Q000009:adverse effects*; D012907:Smoking; D013267:Stilbenes / Q000494:pharmacology*
D018840:HSP70 Heat-Shock Proteins; D020011:Protective Agents; D034741:RNA, Small Interfering; D012906:Smoke; D013267:Stilbenes; C050087:oxygen-regulated proteins; D056564:Sirtuin 1; D000077185:Resveratrol