Oenanthe javanica extract increases immunoreactivities of antioxidant enzymes in the rat kidney.
BACKGROUND
Oenanthe javanica is an aquatic perennial herb originated from East Asia. Nowadays, the effects of Oenanthe javanica have been proven in various disease models. Studies regarding the antioxidant effect of Oenanthe javanica in the kidney are still unclear.


METHODS
This study was therefore performed to investigate the effect of the Oenanthe javanica extract (OJE) in the rat kidney using immunohistochemistry for antioxidant enzymes, copper, zinc-superoxide dismutase (SOD1), manganese superoxide dismutase (SOD2), catalase (CAT) and glutathione peroxidase (GPx). Sprague-Dawley rats were randomly assigned to three groups: (1) normal diet fed-group (normal-group), (2) diet containing ascorbic acid (AA)-fed group (AA-group) as a positive control, (3) diet containing OJE-fed group (OJE-group). AA and OJE were supplied during 28 days.


RESULTS
The side-effects were not observed in all the groups. Immunoreactivities of SOD1, SOD2, CAT and GPx were easily detected in the distal tubules of the kidney, and their immunoreactivities in the AA-and OJE-groups were increased to about 1.4-1.5 times and 2 times, respectively, compared with those in the normal-group.


CONCLUSIONS
OJE significantly increased expressions of SOD1 & 2, CAT and GPx immunoreactivities in the distal tubules of the rat kidney, and this finding suggests that significant enhancements of endogenous enzymatic antioxidants by OJE treatment may be a legitimate strategy for decreasing oxidative stresses in the kidney.
Department of Biomedical Science and Research Institute for Bioscience and Biotechnology, Hallym University, Chuncheon 200-702, South Korea.
Hyun-Jin
HJ
Tae
Department of Neurobiology, School of Medicine, Kangwon National University, Chuncheon 200-701, South Korea.
Joon Ha
JH
Park
Department of Neurobiology, School of Medicine, Kangwon National University, Chuncheon 200-701, South Korea.
Jeong-Hwi
JH
Cho
Department of Neurobiology, School of Medicine, Kangwon National University, Chuncheon 200-701, South Korea.
In Hye
IH
Kim
Department of Neurobiology, School of Medicine, Kangwon National University, Chuncheon 200-701, South Korea.
Ji Hyeon
JH
Ahn
Department of Neurobiology, School of Medicine, Kangwon National University, Chuncheon 200-701, South Korea.
Jae Chul
JC
Lee
Department of Food Science and Biotechnology, College of Agriculture and Life Sciences, Kangwon National University, Chuncheon 200-701, South Korea.
Jong-Dai
JD
Kim
Department of Biomedical Science and Research Institute for Bioscience and Biotechnology, Hallym University, Chuncheon 200-702, South Korea.
Jinseu
J
Park
Department of Biomedical Science and Research Institute for Bioscience and Biotechnology, Hallym University, Chuncheon 200-702, South Korea.
Soo Young
SY
Choi
Department of Neurobiology, School of Medicine, Kangwon National University, Chuncheon 200-701, South Korea. Email: mhwon@kangwon.ac.kr.
Moo-Ho
MH
Won
D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D002374:Catalase / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D007668:Kidney / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism*; D008297:Male; D031050:Oenanthe / Q000737:chemistry*; D018384:Oxidative Stress / Q000187:drug effects; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013482:Superoxide Dismutase / Q000378:metabolism; D000099156:Superoxide Dismutase 2
D000975:Antioxidants; D002374:Catalase; D005979:Glutathione Peroxidase; D010936:Plant Extracts; D013482:Superoxide Dismutase; D000099156:Superoxide Dismutase 2