2DE maps in the discovery of human autoimmune kidney diseases: the case of membranous glomerulonephritis.
The identification of antigens in the autoimmune diseases is a primary point to elucidate the pathogenesis of disease. Here, we propose an "in vivo" proteomics approach to identify the antigens of auto-antibodies in membranous glomerulonephritis. In this approach, podocyte proteins resolved by two-dimensional electrophoresis were semidry blotted to nitrocellulose membrane. Then the antibodies eluted from microdissected glomeruli and serum samples were used as a probe for the detection of podocyte antigens and characterized by means of mass spectrometry. These combined methods allowed us to identify six new antigens in membranous glomerulonephritis.
Laboratory on Pathophysiology of Uremia, Istituto Giannina Gaslini, Largo G. Gaslini 5, Genoa, Italy.
Maurizio
M
Bruschi
Laura
L
Santucci
Gian Marco
GM
Ghiggeri
Giovanni
G
Candiano
D000906:Antibodies / Q000032:analysis; D002460:Cell Line; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel; D015433:Glomerulonephritis, Membranous / Q000175:diagnosis* / Q000378:metabolism / Q000473:pathology; D006801:Humans; D007091:Image Processing, Computer-Assisted; D015151:Immunoblotting; D050199:Podocytes / Q000378:metabolism; D040901:Proteomics; D012394:Rosaniline Dyes / Q000378:metabolism; D013194:Staining and Labeling
D000906:Antibodies; D012394:Rosaniline Dyes; C048139:Coomassie blue