Response surface methodology: optimisation of antifungal bioemulsifier from novel Bacillus thuringiensis.
An antifungal bioemulsifier compound was produced from a novel strain of Bacillus thuringiensis pak2310. To accentuate the production and as the first step to improve the yield, a central composite design (CCD) was used to study the effect of various factors like minimal salts (1X and 3X), glycerol concentration (2% and 4%), beef extract concentration (1% and 3%), and sunflower oil concentration (2% and 4%) on the production of bioemulsifier molecule and to optimize the conditions to increase the production. The E 24 emulsification index was used as the response variable as the increase in surfactant production was seen to be proportional to increased emulsification. A quadratic equation was employed to express the response variable in terms of the independent variables. Statistical tools like student's t-test, F-test, and ANOVA were employed to identify the important factors and to test the adequacy of the model. Under optimum conditions (1X concentration of minimal salts (MS), 2.6% glycerol (v/v), 1% beef extract (w/v), and 2% sunflower oil (v/v)) a 65% increase in yield was produced.
School of Chemical and Biotechnology, SASTRA University, Thirumalaisamudram, Thanjavur, Tamil Nadu 613401, India.
Deepak
0000-0002-0577-5169
D
Rajendran
School of Chemical and Biotechnology, SASTRA University, Thirumalaisamudram, Thanjavur, Tamil Nadu 613401, India.
Ponnusami
P
Venkatachalam
Centre for Research on Infectious Diseases (CRID), School of Chemical and Biotechnology, SASTRA University, Thirumalaisamudram, Thanjavur, Tamil Nadu 613401, India.
Jayapradha
0000-0002-1838-9718
J
Ramakrishnan
D000818:Animals; D000935:Antifungal Agents / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D001413:Bacillus thuringiensis / Q000737:chemistry* / Q000378:metabolism; D002417:Cattle; D045424:Complex Mixtures / Q000737:chemistry; D003470:Culture Media / Q000737:chemistry; D004655:Emulsions; D005163:Factor Analysis, Statistical; D005670:Fusarium / Q000187:drug effects / Q000254:growth & development; D005990:Glycerol / Q000737:chemistry; D008826:Microbial Sensitivity Tests; D010938:Plant Oils / Q000737:chemistry; D000074242:Sunflower Oil; D013501:Surface-Active Agents / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology
D000935:Antifungal Agents; D045424:Complex Mixtures; D003470:Culture Media; D004655:Emulsions; D010938:Plant Oils; D000074242:Sunflower Oil; D013501:Surface-Active Agents; D005990:Glycerol