Collection of 125 oocytes in an in vitro maturation cycle using a new oocyte collection technique: a case report.
BACKGROUND
We have developed a new oocyte collection technique applicable to use in women with "string-of-pearls" polycystic ovaries undergoing in vitro maturation (IVM) of oocytes for in vitro fertilization.


METHODS
A 34-year-old woman with polycystic ovary syndrome and infertility underwent IVM. Her ovaries had the string-of-pearls appearance on ultrasound, and antral follicle counts were consistently less than 60. An IVM cycle was performed using a new "rapid-pass" oocyte collection technique. We retrieved 125 germinal vesicle oocytes. A total of 44 oocytes reached the metaphase II stage after 48 hours in culture. After fertilization, four embryos were transferred to the uterus, resulting in a live birth.


CONCLUSIONS
We believe this to be the largest number of oocytes retrieved from a single individual at one time. This was done using a newly developed aspiration technique.
McGill University Health Centre Reproductive Centre, Royal Victoria Hospital, Montreal QC.
Michael H
MH
Dahan
McGill University Health Centre Reproductive Centre, Royal Victoria Hospital, Montreal QC; Assisted Reproduction Unit, Department of Obstetrics and Gynecology, Uludag University, Bursa, Turkey.
Baris
B
Ata
McGill Medical School, Montreal QC.
Resa
R
Rosenberg
McGill University Health Centre Reproductive Centre, Royal Victoria Hospital, Montreal QC.
Jin Tae
JT
Chung
McGill University Health Centre Reproductive Centre, Royal Victoria Hospital, Montreal QC.
Weon Young
WY
Son
McGill University Health Centre Reproductive Centre, Royal Victoria Hospital, Montreal QC.
Seang Lin
SL
Tan
D000328:Adult; D005260:Female; D006801:Humans; D054315:Oocyte Retrieval; D010053:Ovary / Q000502:physiology*; D011085:Polycystic Ovary Syndrome / Q000473:pathology*; D011247:Pregnancy; D047929:Term Birth
IVM; in vitro maturation; polycystic ovaries; record egg retrieval