Mediators of mast cells in bullous pemphigoid and dermatitis herpetiformis.
Bullous pemphigoid (BP) and dermatitis herpetiformis (DH) are skin diseases associated with inflammation. However, few findings exist concerning the role of mast cells in autoimmune blistering disease. Skin biopsies were taken from 27 BP and 14 DH patients, as well as 20 healthy individuals. Immunohistochemistry was used to identify the localization and mast cell expression of TNFα and MMP9 in skin lesions and perilesional skin. The serum concentrations of TNFα, MMP9, chymase, tryptase, PAF, and IL-4 were measured by immunoassay. TNFα and MMP9 expression in the epidermis and in inflammatory influxed cells in the dermis was detected in skin biopsies from patients. Although these mediators were found to be expressed in the perilesional skin of all patients, the level was much lower than that in lesional skin. Increased serum PAF levels were observed in BP patients. Mast cells may play an essential role in activating inflammation, which ultimately contributes to the tissue damage observed in BP and DH. Our findings suggest that differences in the pattern of cytokine expression directly contribute to variations in cellular infiltration in DH and BP.
Department of Dermatology and Venereology, Medical University of Lodz, Plac Hallera 1, 90-497 Lodz, Poland.
Agnieszka
0000-0002-4548-3080
A
Zebrowska
Laboratory of Nephropathology of Medical University of Lodz, Pomorska 251, 92-213 Lodz, Poland.
Malgorzata
0000-0002-6265-307X
M
Wagrowska-Danilewicz
Laboratory of Nephropathology of Medical University of Lodz, Pomorska 251, 92-213 Lodz, Poland.
Marian
0000-0003-0732-406X
M
Danilewicz
Laboratory of Nephropathology of Medical University of Lodz, Pomorska 251, 92-213 Lodz, Poland.
Olga
O
Stasikowska-Kanicka
Department of Dermatology and Venereology, Medical University of Lodz, Plac Hallera 1, 90-497 Lodz, Poland.
Lilianna
L
Kulczycka-Siennicka
Department of Dermatology and Venereology, Medical University of Lodz, Plac Hallera 1, 90-497 Lodz, Poland.
Anna
A
Wozniacka
Department of Dermatology and Venereology, Medical University of Lodz, Plac Hallera 1, 90-497 Lodz, Poland.
Elzbieta
E
Waszczykowska
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D016022:Case-Control Studies; D018925:Chemokines / Q000097:blood; D003874:Dermatitis Herpetiformis / Q000097:blood / Q000201:enzymology / Q000276:immunology*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D018836:Inflammation Mediators / Q000097:blood / Q000378:metabolism*; D008297:Male; D008407:Mast Cells / Q000276:immunology*; D020780:Matrix Metalloproteinase 9 / Q000097:blood / Q000378:metabolism; D008875:Middle Aged; D010391:Pemphigoid, Bullous / Q000097:blood / Q000201:enzymology / Q000276:immunology*; D010972:Platelet Activating Factor / Q000378:metabolism; D012867:Skin / Q000201:enzymology / Q000276:immunology; D014409:Tumor Necrosis Factor-alpha / Q000097:blood / Q000378:metabolism; D055815:Young Adult
D018925:Chemokines; D018836:Inflammation Mediators; D010972:Platelet Activating Factor; D014409:Tumor Necrosis Factor-alpha; C579270:MMP9 protein, human; D020780:Matrix Metalloproteinase 9