Effects of engineered conserved dopamine neurotrophic factor-expressing bone marrow stromal cells on dopaminergic neurons following 6-OHDA administrations.
Numerous lines of evidence previously indicated that conserved dopamine neurotrophic factor (CDNF) has potential therapeutic value for Parkinson's disease (PD); however, this hypothesis remains controversial. In the present study, the therapeutic effects of engineered CDNF-expressing bone marrow stromal cells (CDNF-BMSCs) on dopaminergic (DA) neurons were evaluated in vivo. CDNF-BMSCs and control BMSCs were transplanted into the rat striatum and one week later, 6-hydroxydopamine (6-OHDA) was administered to induce neurotoxicity. It was discovered that intrastriatal transplantation of CDNF-BMSCs significantly reduced 6-OHDA-induced neurotoxicity in DA neurons with regard to behavioral recovery and tyrosine hydroxylase levels in the substantia nigra and striatum. These data therefore indicated that transplantation of engineered CDNF-BMSCs into the striatum may present a beneficial strategy for the treatment of PD.
Department of Neurosurgery, Anhui Provincial Hospital Affiliated to Anhui Medical University, Anhui Province Key Laboratory of Brain Function and Brain Diseases, Hefei, Anhui 230001, P.R. China.
Jiaming
J
Mei
Department of Neurosurgery, Anhui Provincial Hospital Affiliated to Anhui Medical University, Anhui Province Key Laboratory of Brain Function and Brain Diseases, Hefei, Anhui 230001, P.R. China.
Chaoshi
C
Niu
D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects; D001854:Bone Marrow Cells / Q000166:cytology*; D003342:Corpus Striatum / Q000378:metabolism; D004195:Disease Models, Animal; D059290:Dopaminergic Neurons / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D005260:Female; D007150:Immunohistochemistry; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D051379:Mice; D009414:Nerve Growth Factors / Q000235:genetics / Q000378:metabolism*; D016627:Oxidopamine / Q000633:toxicity*; D010300:Parkinson Disease / Q000378:metabolism / Q000473:pathology; D051381:Rats; D017207:Rats, Sprague-Dawley; D017208:Rats, Wistar
C577430:CDNF protein, rat; D009414:Nerve Growth Factors; D016627:Oxidopamine