Toll-like receptors 2 and 4 in pigs' kidneys early after autotransplantation procedure.
OBJECTIVE
The aim of this paper was to evaluate mRNA expression of Toll-like receptors 2 (TLR2) and 4 (TLR4) and the adaptor protein myeloid differentiation primary-response protein 88 (MyD88) in pigs' kidneys 14 and 30 days after autotransplantation.


METHODS
The research was conducted on 12 animals that underwent left renal transplantation procedure with further standardized rinsing with Biolasol solution and 24 hours' storage in 4°C; subsequently the kidneys were implanted in the right retroperitoneal space after right-side nephrectomy. Six randomly chosen animals (group I) were under observation for 14 days, the other 6 (group II) for 30 days. After these observation periods, the animals were killed and 4-g samples were collected from the renal cortex and medulla.


RESULTS
Expression of mRNA in homogenates of collected samples were determined with the use of reverse-transcription polymerase chain reaction analysis. Obtained results in both groups, presented in relation to GAPDH, were compared with the use of Mann-Whitney U test. Stable graft function was observed in all animals from the 2nd day after the procedure. TLR2 in group I reached the mean value of 3.64 and was statistically significantly higher than in group II (2.19). Inverse proportion was observed in case of mRNA for TLR4: group II presented 2 times higher value than group I (0.25 vs 0.11). Similarly, significant difference was observed in MyD88 (group I, 0.067; group II, 0.45).


CONCLUSIONS
At 14 days after autotransplantation of a pig kidney, mRNA expression for TLR2 is dominant; later, expression increases for TLR4 and MyD88.
Department of General, Vascular, and Transplant Surgery, Medical University of Silesia, Katowice, Poland. Electronic address: acaban@man.pl.
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Caban
Department of General, Vascular, and Transplant Surgery, Medical University of Silesia, Katowice, Poland.
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Oczkowicz
Department of General, Vascular, and Transplant Surgery, Medical University of Silesia, Katowice, Poland.
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Budziński
Department of Histology and Embryology, Medical University of Silesia, Katowice, Poland.
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Suszka-Świtek
Department of Applied Pharmacy and Drug Technology, Sosnowiec, Poland.
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Dolińska
Department of Applied Pharmacy and Drug Technology, Sosnowiec, Poland.
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Ostróżka-Cieślik
National Research Institute of Animal Production, Kraków-Balice, Poland.
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Wieczorek
"Biochefa" Pharmaceutical Research Production Plant, Sosnowiec, Poland.
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Ryszka
Department of Histology and Embryology, Medical University of Silesia, Katowice, Poland.
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Wiaderkiewicz
Department of General, Vascular, and Transplant Surgery, Medical University of Silesia, Katowice, Poland.
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Cierpka
D000818:Animals; D007668:Kidney / Q000378:metabolism*; D016030:Kidney Transplantation; D053594:Myeloid Differentiation Factor 88 / Q000235:genetics*; D009926:Organ Preservation; D012333:RNA, Messenger / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013552:Swine; D051195:Toll-Like Receptor 2 / Q000235:genetics*; D051197:Toll-Like Receptor 4 / Q000235:genetics*; D014182:Transplantation, Autologous
D053594:Myeloid Differentiation Factor 88; D012333:RNA, Messenger; D051195:Toll-Like Receptor 2; D051197:Toll-Like Receptor 4