Multilayered polyelectrolyte microcapsules: interaction with the enzyme cytochrome C oxidase.
Cell-sized polyelectrolyte capsules functionalized with a redox-driven proton pump protein were assembled for the first time. The interaction of polyelectrolyte microcapsules, fabricated by electrostatic layer-by-layer assembly, with cytochrome c oxidase molecules was investigated. We found that the cytochrome c oxidase retained its functionality, that the functionalized microcapsules interacting with cytochrome c oxidase were permeable and that the permeability characteristics of the microcapsule shell depend on the shell components. This work provides a significant input towards the fabrication of an integrated device made of biological components and based on specific biomolecular functions and properties.
Department of Informatics, Bioengineering, Robotics and Systems Engineering, University of Genova, Genova, Italy.
Laura
L
Pastorino
Department of Informatics, Bioengineering, Robotics and Systems Engineering, University of Genova, Genova, Italy.
Elena
E
Dellacasa
Department of Chemical and Environmental Sciences, University of Limerick, Limerick, Ireland; Materials and Surface Science Institute, University of Limerick, Limerick, Ireland.
Mohamed R
MR
Noor
Department of Chemical and Environmental Sciences, University of Limerick, Limerick, Ireland; Materials and Surface Science Institute, University of Limerick, Limerick, Ireland.
Tewfik
T
Soulimane
Nanophysics, Istituto Italiano di Tecnologia, Genova, Italy.
Paolo
P
Bianchini
Nanophysics, Istituto Italiano di Tecnologia, Genova, Italy.
Francesca
F
D'Autilia
PlasmaChem GmbH, Berlin, Germany.
Alexei
A
Antipov
Nanophysics, Istituto Italiano di Tecnologia, Genova, Italy.
Alberto
A
Diaspro
Materials and Surface Science Institute, University of Limerick, Limerick, Ireland.
Syed A M
SA
Tofail
Department of Informatics, Bioengineering, Robotics and Systems Engineering, University of Genova, Genova, Italy.
Carmelina
C
Ruggiero
D001426:Bacterial Proteins / Q000737:chemistry*; D002214:Capsules; D003576:Electron Transport Complex IV / Q000737:chemistry*; D004800:Enzymes, Immobilized / Q000737:chemistry*; D016963:Thermus thermophilus / Q000201:enzymology*
D001426:Bacterial Proteins; D002214:Capsules; D004800:Enzymes, Immobilized; D003576:Electron Transport Complex IV