Collyricloides massanae (Digenea, Collyriclidae): spermatozoon ultrastructure and phylogenetic importance.
The spermatological characteristics of Collyricloides massanae (Digenea: Collyriclidae), a parasite of Apodemus sylvaticus caught in France, were studied by means of transmission electron microscopy. The mature sperm of C. massanae presents two axonemes of different lengths with the 9 + "1" pattern of the Trepaxonemata, two bundles of parallel cortical microtubules, external ornamentation of the plasma membrane, spine-like bodies, one mitochondrion, a nucleus and granules of glycogen. An analysis of spermatological organisation emphasised some differences between the mature spermatozoon of C. massanae and those reported in the Gorgoderoidea species studied to date, specially belonging to the families Dicrocoeliidae, Paragonimidae and Troglotrematidae. The ultrastructural criteria described in C. massanae such as the morphology of both anterior and posterior spermatozoon extremities, the association "external ornamentation+cortical microtubules", the type 2 of external ornamentation and the spine-like bodies would allow us to bring closer the Collyriclidae to Microphalloidea. However, further ultrastructural and molecular studies are needed particularly in the unexplored taxa in order to fully resolve the phylogenetic position of the Collyriclidae.
CNRS - University of Corsica, UMR SPE 6134, SERME "Service d'Étude et de Recherche en Microscopie Électronique", 20250 Corte, France - Laboratory of Evolutionary Biology, Ecology and Management of Ecosystems, Faculty of Sciences and Techniques, Cheikh Anta Diop University of Dakar, BP 5055, Dakar, Senegal.
Abdoulaye Jacque
AJ
Bakhoum
CNRS - University of Corsica, UMR SPE 6134, SERME "Service d'Étude et de Recherche en Microscopie Électronique", 20250 Corte, France.
Yann
Y
Quilichini
Laboratori de Parasitologia, Departament de Microbiologia i Parasitologia Sanitàries, Facultat de Farmàcia, Universitat de Barcelona, Av. Joan XXIII, sn, 08028 Barcelona, Spain - Institut de Recerca de la Biodiversitat, Facultat de Biologia, Universitat de Barcelona, Av. Diagonal 645, 08028 Barcelona, Spain.
Jordi
J
Miquel
Laboratori de Parasitologia, Departament de Microbiologia i Parasitologia Sanitàries, Facultat de Farmàcia, Universitat de Barcelona, Av. Joan XXIII, sn, 08028 Barcelona, Spain - Institut de Recerca de la Biodiversitat, Facultat de Biologia, Universitat de Barcelona, Av. Diagonal 645, 08028 Barcelona, Spain.
Carlos
C
Feliu
Laboratory of Evolutionary Biology, Ecology and Management of Ecosystems, Faculty of Sciences and Techniques, Cheikh Anta Diop University of Dakar, BP 5055, Dakar, Senegal.
Cheikh Tidiane
CT
Bâ
CNRS - University of Corsica, UMR SPE 6134, SERME "Service d'Étude et de Recherche en Microscopie Électronique", 20250 Corte, France.
Bernard
B
Marchand
D000818:Animals; D054468:Axoneme / Q000648:ultrastructure; D005602:France; D008297:Male; D008870:Microtubules / Q000648:ultrastructure; D008928:Mitochondria / Q000648:ultrastructure; D051189:Murinae / Q000469:parasitology; D010802:Phylogeny; D012376:Rodent Diseases / Q000469:parasitology; D013094:Spermatozoa / Q000648:ultrastructure*; D014200:Trematoda / Q000145:classification / Q000166:cytology*; D014201:Trematode Infections / Q000469:parasitology / Q000662:veterinary