Transient effect of single dose exposure of Nigerian Bonny-light crude oil on testicular steroidogenesis in Wistar rats is accompanied by oxidative stress.
The folkloric use of Nigerian Bonny-light crude oil (BLCO) in Niger Delta area of Nigeria is a common practice. There is increasing experimental evidence portending the adverse effects of BLCO an environmental toxicant on testicular function. We investigated the effects of single dose of BLCO (800 mg/kg body weight) on the activities of steroidogenic and antioxidant enzymes such as serum follicle stimulating hormone (FSH), luteinizing hormone (LH) and testosterone, 3 β-hydroxy-steroid dehydrogenase (3 β-HSD), 17 β-hydroxy-steroid dehydrogenase (17 β-HSD), superoxide dismutase (SOD), glutathione-S-transferase (GST) and glutathione peroxidase (GSH-Px), levels of lipid peroxidation (LPO), glutathione reduced (GSH) and steroidogenic acute regulatory (StAR) protein, in testes of rats. There was a sequential reduction in the concentration of steroid hormones and activities of steroidogenic enzymes with a concomitant decrease in levels of StAR protein, followed by a parallel increase in antioxidant enzyme activities and levels of LPO. These findings revealed inhibitory effects of BLCO on testicular steroidogenesis and the possible role of oxidative stress in testicular dysfunction observed in this study.
a Department of Biochemistry, Molecular Drug Metabolism & Toxicology Research Laboratories , College of Medicine, University of Ibadan , Ibadan , Nigeria and.|b Department of Biochemistry and Molecular Biology, Reproductive and Endocrine Biochemistry Research Laboratories , School of Life Sciences, Pondicherry University , Pondicherry , India.
Azubuike Peter
AP
Ebokaiwe
b Department of Biochemistry and Molecular Biology, Reproductive and Endocrine Biochemistry Research Laboratories , School of Life Sciences, Pondicherry University , Pondicherry , India.
Parjapath
P
Ramesh
b Department of Biochemistry and Molecular Biology, Reproductive and Endocrine Biochemistry Research Laboratories , School of Life Sciences, Pondicherry University , Pondicherry , India.
Premendu Prakash
PP
Mathur
a Department of Biochemistry, Molecular Drug Metabolism & Toxicology Research Laboratories , College of Medicine, University of Ibadan , Ibadan , Nigeria and.
Ebenezer Olatunde
EO
Farombi
D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D005640:Follicle Stimulating Hormone / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D007986:Luteinizing Hormone / Q000378:metabolism; D008297:Male; D016488:Medicine, African Traditional / Q000009:adverse effects; D009549:Nigeria; D018384:Oxidative Stress / Q000187:drug effects*; D010578:Petroleum / Q000633:toxicity*; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000378:metabolism; D013737:Testis / Q000187:drug effects* / Q000378:metabolism; D013739:Testosterone / Q000378:metabolism
D000975:Antioxidants; D010578:Petroleum; D013739:Testosterone; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone; D013482:Superoxide Dismutase; D005978:Glutathione
Bonny-light crude oil; oxidative stress; rats; steroidogenesis; testes