Laccase production and metabolic diversity among Flammulina velutipes strains.
Twelve Flammulina velutipes strains originating from Poland were identified using internal transcribed spacer (ITS) region sequencing. Based on the sequences obtained, the genomic relationship of the analyzed strains was determined. All F. velutipes strains were also characterized using Biolog FF MicroPlates to obtain data on C-substrate utilization and mitochondrial activity. The ability to decompose various substrates differed among the F. velutipes strains up to five times. The highest catabolic activities were characteristic for only two strains with capabilities to decompose up to 22 carbon sources. The correlation between carbon repression and laccase production by F. velutipes was analyzed based on glucose assimilation by these strains. Moreover, the influence of metal ions (Cu(2+), Cd(2+)), veratric and ferulic acids, and temperature on laccase activities in the analyzed strains was determined. The results obtained proved that all the inducers influenced laccase expression in almost all the analyzed strains. However, the degree of induction depended not only on the strain used but also on the day of the induction.
Department of Biochemistry, Maria Curie-Skłodowska University, Akademicka 19 St., 20-033, Lublin, Poland, gjanusz@poczta.umcs.lublin.pl.
Grzegorz
G
Janusz
Aleksandra
A
Czuryło
Magdalena
M
Frąc
Beata
B
Rola
Justyna
J
Sulej
Anna
A
Pawlik
Marek
M
Siwulski
Jerzy
J
Rogalski
D016000:Cluster Analysis; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000737:chemistry / Q000235:genetics; D055393:Flammulina / Q000201:enzymology* / Q000378:metabolism*; D042845:Laccase / Q000378:metabolism*; D008969:Molecular Sequence Data; D016533:Mycological Typing Techniques; D010802:Phylogeny; D011044:Poland; D017422:Sequence Analysis, DNA
D004271:DNA, Fungal; D021903:DNA, Ribosomal Spacer; D042845:Laccase