Abrogation of the interferon response promotes more efficient human cytomegalovirus replication.
The effect of abrogating the interferon (IFN) response on human cytomegalovirus (HCMV) replication was investigated using primary human cells engineered to block either the production of or the response to type I IFNs. In IFN-deficient cells, HCMV produced larger plaques and spread and replicated more rapidly than in parental cells. These cells demonstrate the vital role of IFNs in controlling HCMV replication and provide useful tools to investigate the IFN response to HCMV.
Discipline of Infectious Diseases and Immunology, Sydney Medical School, University of Sydney, Camperdown, NSW, Australia.
Brian P
BP
McSharry
Discipline of Infectious Diseases and Immunology, Sydney Medical School, University of Sydney, Camperdown, NSW, Australia Centre for Virus Research, Westmead Millennium Institute, Westmead, NSW, Australia.
Simone K
SK
Forbes
Discipline of Infectious Diseases and Immunology, Sydney Medical School, University of Sydney, Camperdown, NSW, Australia.
Selmir
S
Avdic
School of Biology, University of St. Andrews, St. Andrews, United Kingdom.
Richard E
RE
Randall
Department of Medical Microbiology, Cardiff Institute of Infection and Immunity, Cardiff University School of Medicine, Cardiff, United Kingdom.
Gavin W G
GW
Wilkinson
Discipline of Infectious Diseases and Immunology, Sydney Medical School, University of Sydney, Camperdown, NSW, Australia.
Allison
A
Abendroth
Discipline of Infectious Diseases and Immunology, Sydney Medical School, University of Sydney, Camperdown, NSW, Australia barry.slobedman@sydney.edu.au.
Barry
B
Slobedman
D002478:Cells, Cultured; D003587:Cytomegalovirus / Q000276:immunology* / Q000502:physiology*; D006801:Humans; D007370:Interferon Type I / Q000172:deficiency / Q000276:immunology*; D010948:Viral Plaque Assay; D014779:Virus Replication
D007370:Interferon Type I