Self‑assembled monolayers of alkanethiolates on surface chemistry groups in osteosarcoma cells.
Cell biomedical behavior is influenced by a number of factors, and the extracellular matrix (ECM) of the cellular microenvironment affects certain cancer cells. In the current study, U‑2OS cells were cultured on gold surfaces modified with different terminal chemical groups [methyl (‑CH3), amino (‑NH2), hydroxyl (‑OH) and carboxyl (‑COOH)]. The results revealed that different chemical surfaces convey different behaviors. The density of the different functional surfaces was confirmed by atomic force microscopy. Cell morphology, proliferation rate and cell cycle were investigated using scanning electron microscopy, cell counting and flow cytometry. In conclusion, the type of chemical group on a biomaterial is an important property for the growth of osteosarcoma cells; ‑NH2 and ‑COOH surfaces sustained visible cell adhesion and promoted cell growth.
Department of Orthopaedics Surgery, The First Clinical Medical College, Southern Medical University, Guangzhou, Guangdong 510515, P.R. China.
Ying-Hu
YH
Deng
Department of Spinal Surgery, Hospital of Orthopedics, Guangzhou General Hospital of Guangzhou Military Command, Guangzhou, Guangdong 510010, P.R. China.
Li-Hua
LH
Li
Department of Materials Science and Engineering, Institute of Regenerative and Biomimetic Materials, Tsinghua University, Beijing 100084, P.R. China.
Jin
J
He
Department of Spinal Surgery, Hospital of Orthopedics, Guangzhou General Hospital of Guangzhou Military Command, Guangzhou, Guangdong 510010, P.R. China.
Mei
M
Li
Department of Spinal Surgery, Hospital of Orthopedics, Guangzhou General Hospital of Guangzhou Military Command, Guangzhou, Guangdong 510010, P.R. China.
Yu
Y
Zhang
Department of Materials Science and Engineering, Institute of Regenerative and Biomimetic Materials, Tsinghua University, Beijing 100084, P.R. China.
Xiu-Mei
XM
Wang
Department of Spinal Surgery, Hospital of Orthopedics, Guangzhou General Hospital of Guangzhou Military Command, Guangzhou, Guangdong 510010, P.R. China.
Fu-Zhai
FZ
Cui
Department of Spinal Surgery, Hospital of Orthopedics, Guangzhou General Hospital of Guangzhou Military Command, Guangzhou, Guangdong 510010, P.R. China.
Hong
H
Xia
D000473:Alkanes / Q000737:chemistry / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D001672:Biocompatible Materials / Q000737:chemistry / Q000494:pharmacology*; D002448:Cell Adhesion / Q000187:drug effects; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D055598:Chemical Phenomena; D005109:Extracellular Matrix / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D008855:Microscopy, Electron, Scanning; D012516:Osteosarcoma / Q000378:metabolism*; D013438:Sulfhydryl Compounds / Q000737:chemistry / Q000494:pharmacology*; D013499:Surface Properties
C510729:11-amino-1-undecanethiol; D000473:Alkanes; D001672:Biocompatible Materials; D013438:Sulfhydryl Compounds