Analysis of trace microcystins in vegetables using solid-phase extraction followed by high performance liquid chromatography triple-quadrupole mass spectrometry.
A selective and sensitive method for the simultaneous detection of three common and hazardous microcystins (microcystins-LR, -RR, and -YR) in various vegetables was established using solid-phase extraction followed by high performance liquid chromatography coupled with mass spectrometry. The methanol-water proportion ratio of the extraction solvent and its acidity, as well as the efficiencies of solid-phase extraction, were evaluated to optimize a pretreatment procedure for extracting the microcystins from 10 vegetable matrices. The limits of detection and quantitation were below 7.5 μg/kg (dw) and 25 μg/kg (dw), respectively, in different vegetable matrices. The recoveries of the microcystins in the 10 vegetable matrices ranged from 61.3 to 117.3%, with RSDs of 0.2-18.3%. The established method was used to analyze 28 field vegetable samples collected from the sides of Lake Dianchi, and microcystin-RR was found in almost all samples at concentrations of 36.4-2352.2 μg/kg (dw).
Key Laboratory of Water/Soil Toxic Pollutants Control and Bioremediation of Guangdong Higher Education Institutions, School of Environment, Jinan University , Guangzhou 510632, China.
Yan-Wen
YW
Li
Xiao-Jing
XJ
Zhan
Lei
L
Xiang
Zhe-Shen
ZS
Deng
Bin-Hui
BH
Huang
Hong-Fei
HF
Wen
Teng-Fei
TF
Sun
Quan-Ying
QY
Cai
Hui
H
Li
Ce-Hui
CH
Mo
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005506:Food Contamination / Q000032:analysis*; D052998:Microcystins / Q000737:chemistry* / Q000302:isolation & purification; D052616:Solid Phase Extraction / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods*; D014675:Vegetables / Q000737:chemistry*
D052998:Microcystins; C078588:microcystin
HPLC-MS/MS; matrix effect; microcystins; simultaneous analysis; vegetable