Low-dose γ-irradiation induces dual radio-adaptive responses depending on the post-irradiation time by altering microRNA expression profiles in normal human dermal fibroblasts.
Exposure to high-dose ionizing radiation, including γ-radiation, induces severe skin disorders. However, the biological consequences and molecular mechanisms responsible for the response of human skin to low-dose γ-radiation (LDR) are largely unknown. In the present study, we demonstrate that LDR (0.1 Gy) induces distinct cellular responses in normal human dermal fibroblasts (NHDFs) depending on the post-irradiation time point. A MTT-based cell viability assay and propidium iodide staining-based cell cycle assay revealed that the viability and proportion of the cells in the G2/M phase were differed at 6 and 24 h post-irradiation. Reverse transcription quantitative PCR (RT-qPCR) revealed that LDR significantly upregulated the mRNA expression of collagen type I alpha 1 (COL1A1), but downregulated the mRNA expression of matrix metalloproteinase 1 (MMP1) at 24 h post-irradiation. MicroRNA (miRNA) microarray analysis further demonstrated that LDR induced changes in the expression profiles of specific miRNAs and that some of the deregulated miRNAs were specific to either the early or late radio-adaptive response. Our results suggest that LDR generates dual radio-adaptive responses depending on the post-irradiation time by altering specific miRNA expression profiles in NHDFs.
Molecular-Targeted Drug Research Center and Korea Institute for Skin and Clinical Sciences, Konkuk University, Seoul 143-701, Republic of Korea.
Seunghee
S
Bae
Molecular-Targeted Drug Research Center and Korea Institute for Skin and Clinical Sciences, Konkuk University, Seoul 143-701, Republic of Korea.
Karam
K
Kim
Molecular-Targeted Drug Research Center and Korea Institute for Skin and Clinical Sciences, Konkuk University, Seoul 143-701, Republic of Korea.
Hwa Jun
HJ
Cha
Molecular-Targeted Drug Research Center and Korea Institute for Skin and Clinical Sciences, Konkuk University, Seoul 143-701, Republic of Korea.
Yeongmin
Y
Choi
Molecular-Targeted Drug Research Center and Korea Institute for Skin and Clinical Sciences, Konkuk University, Seoul 143-701, Republic of Korea.
Shang Hun
SH
Shin
Molecular-Targeted Drug Research Center and Korea Institute for Skin and Clinical Sciences, Konkuk University, Seoul 143-701, Republic of Korea.
In-Sook
IS
An
Laboratory of Molecular Oncology, Cheil General Hospital and Women's Healthcare Center, Kwandong University, College of Medicine, Seoul 100-380, Republic of Korea.
Jae Ho
JH
Lee
Department of Chemistry, Hanyang University, Seoul 133-791, Republic of Korea.
Su Jae
SJ
Lee
Radiation Effect Research Team, Radiation Health Research Institute, Korea Hydro and Nuclear Power Co., Ltd., Seoul 132-703, Republic of Korea.
Ji Young
JY
Kim
Radiation Effect Research Team, Radiation Health Research Institute, Korea Hydro and Nuclear Power Co., Ltd., Seoul 132-703, Republic of Korea.
Seon Young
SY
Nam
Molecular-Targeted Drug Research Center and Korea Institute for Skin and Clinical Sciences, Konkuk University, Seoul 143-701, Republic of Korea.
Sungkwan
S
An
D000220:Adaptation, Biological / Q000235:genetics; D002453:Cell Cycle / Q000235:genetics / Q000528:radiation effects; D059447:Cell Cycle Checkpoints / Q000235:genetics / Q000528:radiation effects; D002470:Cell Survival / Q000235:genetics / Q000528:radiation effects; D016000:Cluster Analysis; D024042:Collagen Type I / Q000235:genetics; D000090602:Collagen Type I, alpha 1 Chain; D019295:Computational Biology; D005347:Fibroblasts / Q000378:metabolism* / Q000528:radiation effects*; D005720:Gamma Rays; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006801:Humans; D020781:Matrix Metalloproteinase 1 / Q000235:genetics; D035683:MicroRNAs / Q000235:genetics*; D034622:RNA Interference; D011829:Radiation Dosage; D059467:Transcriptome
C000716127:COL1A1 protein, human; D024042:Collagen Type I; D000090602:Collagen Type I, alpha 1 Chain; D035683:MicroRNAs; D020781:Matrix Metalloproteinase 1