Targeted allelic expression profiling in human islets identifies cis-regulatory effects for multiple variants identified by type 2 diabetes genome-wide association studies.
Genome-wide association studies (GWAS) have identified variation at >65 genomic loci associated with susceptibility to type 2 diabetes, but little progress has been made in elucidating the molecular mechanisms behind most of these associations. Using samples heterozygous for transcribed single nucleotide polymorphisms (SNPs), allelic expression profiling is a powerful technique for identifying cis-regulatory variants controlling gene expression. In this study, exonic SNPs, suitable for measuring mature mRNA levels and in high linkage disequilibrium with 65 lead type 2 diabetes GWAS SNPs, were identified and allelic expression determined by real-time PCR using RNA and DNA isolated from islets of 36 white nondiabetic donors. A significant allelic expression imbalance (AEI) was identified for 7/14 (50%) genes tested (ANPEP, CAMK2B, HMG20A, KCNJ11, NOTCH2, SLC30A8, and WFS1), with significant AEI confirmed for five of these genes using other linked exonic SNPs. Lastly, results of a targeted islet expression quantitative trait loci experiment support the AEI findings for ANPEP, further implicating ANPEP as the causative gene at its locus. The results of this study support the hypothesis that changes to cis-regulation of gene expression are involved in a large proportion of SNP associations with type 2 diabetes susceptibility.
Institute of Biomedical & Clinical Science, University of Exeter Medical School, Exeter, U.K.
Jonathan M
JM
Locke
Institute of Biomedical & Clinical Science, University of Exeter Medical School, Exeter, U.K.
Gerald
G
Hysenaj
Institute of Biomedical & Clinical Science, University of Exeter Medical School, Exeter, U.K.
Andrew R
AR
Wood
Institute of Biomedical & Clinical Science, University of Exeter Medical School, Exeter, U.K.
Michael N
MN
Weedon
Institute of Biomedical & Clinical Science, University of Exeter Medical School, Exeter, U.K. l.w.harries@exeter.ac.uk.
Lorna W
LW
Harries
D000483:Alleles; D022981:Allelic Imbalance; D003924:Diabetes Mellitus, Type 2 / Q000235:genetics*; D005091:Exons; D020869:Gene Expression Profiling; D056426:Genetic Loci; D055106:Genome-Wide Association Study; D006801:Humans; D050436:Regulatory Elements, Transcriptional