Sample treatment methods involving combinatorial peptide ligand libraries for improved proteomes analyses.
If used in an optimized manner, the technology of combinatorial peptide solid-phase libraries can easily improve the analytical determinations of proteomes by several factors. The discovery of novel species and of early stage biomarkers becomes thus reachable with a simple sample treatment. This report describes the most important point to consider (overloading and full recovery) along with a minimum scientific background and gives then detailed recipes to laboratory technicians. Orientations for optional routes are also given according to the objective of the experimental investigations. This covers different approaches to capture proteins of very low abundance. Total protein harvestings to prevent partial losses are also described such as single exhaustive desorption and fractionated elutions for more detailed analyses. Documented results are also reported demonstrating the capability of the technology well beyond what is the common assumption.
Department of Chemistry, Materials and Chemical Engineering "Giulio Natta", Politecnico di Milano, Via Mancinelli 7, Milan, 20131, Italy.
Pier Giorgio
PG
Righetti
Egisto
E
Boschetti
D053000:Analytic Sample Preparation Methods / Q000379:methods*; D002021:Buffers; D020650:Combinatorial Chemistry Techniques / Q000379:methods*; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D019151:Peptide Library; D011506:Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D040901:Proteomics / Q000379:methods*; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry; D012995:Solubility; D014867:Water / Q000737:chemistry
D002021:Buffers; D019151:Peptide Library; D011506:Proteins; D014867:Water; D012967:Sodium Dodecyl Sulfate