Preparation of lysine-coated magnetic Fe2O3 nanoparticles and influence on viability of A549 lung cancer cells.
OBJECTIVE
To explore the effect of lysine-coated oxide magnetic nanoparticles (Lys@MNPs) on viability and apoptosis of A549 lung cancer cells.


METHODS
Transmission electron microscopy (TEM), vibrating sample magnetometer (VSM) and Zeta potentiometric analyzer were employed to characterize Lys@MNPs. Then Lys@MNPs and lung cancer A549 cells were co-cultured to study the effect of Lys@MNPs on cell viability and apoptosis. The pathway of Lys@MNPs entering A549 cells was detected by TEM and cell imaging by 1.5 T MRI.


RESULTS
Lys@MNPs were 10.2 nm in grain diameter, characterized by small size, positive charge, and superparamagnetism. Under low-dose concentration of Lys@MNPs (<40 μg/mL), the survival rate of A549 cells was decreased but remained higher than 95% while under high-dose concentration (100 μg/mL), the survival ratewas still higher than 80%, which suggested Lys@MNPs had limited influence on the viability of A549 cells, with good biocompatibility and and no induction of apoptosis. Moreover, high affinity for cytomembranes, was demonstrated presenting good imaging effects.


CONCLUSIONS
Lys@MNPs can be regarded as a good MRI negative contrast agents, with promising prospects in biomedicine.
Department of Clinical Laboratory Medicine, Linyi People's Hospital, Linyi, Shandong, China E-mail : yunerniyunerni @163.com.
Yu-Hua
YH
Ma
Hai-Ying
HY
Peng
Rui-Xia
RX
Yang
Fang
F
Ni
D017209:Apoptosis; D049109:Cell Proliferation; D003287:Contrast Media; D005290:Ferric Compounds / Q000737:chemistry*; D006801:Humans; D008175:Lung Neoplasms / Q000473:pathology*; D008239:Lysine / Q000737:chemistry*; D008279:Magnetic Resonance Imaging; D058185:Magnetite Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D046529:Microscopy, Electron, Transmission; D014407:Tumor Cells, Cultured
D003287:Contrast Media; D005290:Ferric Compounds; D058185:Magnetite Nanoparticles; C000499:ferric oxide; D008239:Lysine