Crystallization and preliminary X-ray diffraction analysis of the S-adenosylhomocysteine hydrolase (SAHH) from Thermotoga maritima.
S-Adenosylhomocysteine hydrolase (SAHH) catalyzes the reversible conversion of S-adenosylhomocysteine into adenosine and homocysteine. The SAHH from Thermotoga maritima (TmSAHH) was expressed in Escherichia coli and the recombinant protein was purified and crystallized. TmSAHH crystals belonging to space group C2, with unit-cell parameters a=106.3, b=112.0, c=164.9 Å, β=103.5°, were obtained by the sitting-drop vapour-diffusion method and diffracted to 2.85 Å resolution. Initial phase determination by molecular replacement clearly indicated that the crystal contains one homotetramer per asymmetric unit. Further refinement of the crystal structure is in progress.
College of Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, People's Republic of China.
Miao
M
He
Industrial Enzymes National Engineering Laboratory, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, People's Republic of China.
Yingying
Y
Zheng
Industrial Enzymes National Engineering Laboratory, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, People's Republic of China.
Chun-Hsiang
CH
Huang
Biofuels Institute, School of Environment, Jiangsu University, Zhenjiang 212013, People's Republic of China.
Guojun
G
Qian
Industrial Enzymes National Engineering Laboratory, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, People's Republic of China.
Xiansha
X
Xiao
Institute of Biological Chemistry, Academia Sinica, Taipei 115, Taiwan.
Tzu-Ping
TP
Ko
Biofuels Institute, School of Environment, Jiangsu University, Zhenjiang 212013, People's Republic of China.
Weilan
W
Shao
Industrial Enzymes National Engineering Laboratory, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, People's Republic of China.
Rey-Ting
RT
Guo
D043383:Adenosylhomocysteinase / Q000737:chemistry* / Q000302:isolation & purification; D001426:Bacterial Proteins / Q000737:chemistry* / Q000302:isolation & purification; D003460:Crystallization; D020124:Thermotoga maritima / Q000201:enzymology*; D014961:X-Ray Diffraction
D001426:Bacterial Proteins; D043383:Adenosylhomocysteinase
S-adenosylhomocysteine hydrolase; Thermotoga maritima; thermostable enzyme