Characterization of a novel β-glucosidase from Gongronella sp. W5 and its application in the hydrolysis of soybean isoflavone glycosides.
A novel β-glucosidase named BglW5 from Gongronella sp. was isolated, purified, and characterized for the first time. Under solid state fermentation, the yield of BglW5 was 49.9 U/g fermented medium. BglW5 was stable over a wide pH range of 3.0-8.5 and retained more than 50% of its maximal activity after incubation at 25 °C for 96 h. The half-lives of BglW5 were 20 h at 60 °C, and 1 h at 70 °C. The activity of BglW5 was stimulated by xylose and fructose at concentrations up to 500 mM, with maximal stimulatory effect of 1.6-fold and 2.2-fold, respectively. BglW5 converted isoflavone glycosides to aglycones, with a hydrolysis rate of 96.2% for daidzin and 96.7% for genistin. The productivities were 1.5 mmol L(-1) h(-1) for daidzein and 1.23 mmol L(-1) h(-1) for genistein, respectively. These features suggest that BglW5 has great application potential in the hydrolysis of soybean isoflavone glycosides.
School of Life Sciences, Anhui University , Hefei, Anhui 230601, China.
Wei
W
Fang
Rui
R
Song
Xinxin
X
Zhang
Xianbing
X
Zhang
Xuecheng
X
Zhang
Xiaotang
X
Wang
Zemin
Z
Fang
Yazhong
Y
Xiao
D055162:Biocatalysis; D004795:Enzyme Stability; D005656:Fungal Proteins / Q000737:chemistry*; D005658:Fungi / Q000201:enzymology*; D006027:Glycosides / Q000737:chemistry*; D007529:Isoflavones / Q000737:chemistry*; D007700:Kinetics; D013025:Glycine max / Q000737:chemistry*; D001617:beta-Glucosidase / Q000737:chemistry*
D005656:Fungal Proteins; D006027:Glycosides; D007529:Isoflavones; D001617:beta-Glucosidase
daidzin; genistin; solid-state fermentation; zygomyzetes