RNAi in the mouse: rapid and affordable gene function studies in a vertebrate system.
The addition of RNA interference (RNAi) to the mammalian genomic toolbox has significantly expanded our ability to use higher-order models in studies of development and disease. The mouse, in particular, has benefited most from RNAi technology. Unique combinations of RNAi vectors and delivery methods now offer a broad platform for gene silencing in transgenic mice, enabling the design of new physiologically relevant models. The era of RNAi mice has accelerated the pace of genetic study and made high-throughput screens not only feasible but also affordable.
Human Biology Division, Fred Hutchinson Cancer Research Center, Seattle, WA, USA.
Julie A
JA
Rytlewski
Slobodan
S
Beronja
D000818:Animals; D055785:Gene Knockdown Techniques / Q000379:methods* / Q000639:trends; D015316:Genetic Therapy / Q000379:methods / Q000639:trends; D005822:Genetic Vectors / Q000235:genetics; D023281:Genomics / Q000379:methods* / Q000639:trends; D006801:Humans; D051379:Mice; D008957:Models, Genetic; D034622:RNA Interference; D014714:Vertebrates / Q000145:classification / Q000235:genetics*