Antioxidant activity and inhibitory effect of some commonly used medicinal plants against lipid per-oxidation in mice brain.
BACKGROUND
The present study compares the protective properties of aqueous extracts of six medicinal plants, Phyllanthus emblica, Terminalia chebula (black and yellow), Terminalia arjuna, Balsamodendron Mukul and Alium sativum against lipid per-oxidation in mice brain.


METHODS
The antioxidant activities were analyzed by lipid per-oxidation assay, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical assay, total antioxidant activity and metal chelation.


RESULTS
The extracts (fruits and bark) showed inhibition against thiobarbituric acid reactive species (TBARS) induced by pro-oxidant (10 µM FeSO4) in mice brain. Moreover, the free radical scavenging activities of the extracts was evaluated by the scavenging of DPPH radical (IC₅₀, 23.23 ± 1.2 µg/ml (Phyllanthus emblica), 20.24 ± 0.9 µg/ml (Terminalia chebula yellow) and 17.33 ± 1.1 µg/ml (Terminalia chebula black), 19.44 ± 0.45 µg/ml (Terminalia arjuna), 56.59 ± 2.1 µg/ml (Balsamodendron Mukul) and < 200 µg/ml (Alium sativum).


CONCLUSIONS
The higher antioxidant and inhibitory effect of Terminalia chebula black in this study could be attributed to its significantly higher phenolic content, Fe(II) chelating ability, reducing ability and free radical scavenging activity. Therefore oxidative stress in brain could be potentially prevented by the intake of these plants.
Faculty of Eastern Medicine, Hamdard University Karachi, Pakistan.
Asmatullah
A
Khan
Faculty of Eastern Medicine, Hamdard University Karachi, Pakistan.
Halima
H
Nazar
Department of Chemistry, University of Poonch Rawalakot A.K Pakistan.
Syed Mubashar
SM
Sabir
Department of Chemistry, University of Poonch Rawalakot A.K Pakistan.
Muhammad
M
Irshad
Department of Plant Breeding and Molecular Genetics, University of Poonch Rawalakot A.K Pakistan.
Shahid Iqbal
SI
Awan
University of Azad Jammu and Kashmir, Muzaffarabad A.K Pakistan.
Rizwan
R
Abbas
Faculty of Medical and Health Sciences, University of Poonch Rawalakot A.K Pakistan.
Muhammad
M
Akram
Department of Plant Breeding and Molecular Genetics, University of Poonch Rawalakot A.K Pakistan.
Abdul
A
Khaliq
Departmento de Química, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Santa Maria, CEP 97105-900, Brazil.
João Batista Texeira
JB
Rocha
University of Azad Jammu and Kashmir, Muzaffarabad A.K Pakistan.
Syed Dilnawaz
SD
Ahmad
National Institute of Health, Islamabad, Pakistan.
Farnaz
F
Malik
D000490:Allium; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001713:Biphenyl Compounds / Q000378:metabolism; D001921:Brain / Q000187:drug effects* / Q000378:metabolism; D029744:Commiphora; D005638:Fruit; D015227:Lipid Peroxidation / Q000187:drug effects*; D019684:Magnoliopsida; D051379:Mice; D016877:Oxidants / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D010636:Phenols / Q000494:pharmacology; D029803:Phyllanthus; D010851:Picrates / Q000378:metabolism; D024301:Plant Bark; D010936:Plant Extracts / Q000494:pharmacology*; D010946:Plants, Medicinal; D029766:Terminalia; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
D000975:Antioxidants; D001713:Biphenyl Compounds; D016877:Oxidants; D010636:Phenols; D010851:Picrates; D010936:Plant Extracts; D017392:Thiobarbituric Acid Reactive Substances; C004931:1,1-diphenyl-2-picrylhydrazyl
Antioxidant activity; Balb c mice; iron chelation; medicinal plants; oxidative stress; phenolics