A fully validated method for the determination of lacosamide in human plasma using gas chromatography with mass spectrometry: application for therapeutic drug monitoring.
A simple gas chromatographic method with mass spectrometry detection was developed and validated for the determination of lacosamide in human plasma. Lacosamide and the internal standard, levetiracetam-d6, were extracted from 200 μL plasma, by a solid-phase extraction through HF Bond Elut C18 columns, and derivatized using N-methyl-N-tert-butyldimethylsilyltrifluoroacetamide with 1% tert-butyldimethylsilylchloride in acetonitrile. The limit of quantification was found to be 0.20 μg/mL and the assay was linear up to 20.0 μg/mL with correlation coefficient ≥0.994. The intra- and interday precision values were <4.1% in terms of relative standard deviation (%) and the values of intra- and interday accuracy were found to be within -7.2 and 5.3% in terms of relative error (%). Absolute recovery of the method for lacosamide was determined at three concentration levels and ranged from 92.5 to 97.6%. The developed method uses small volumes of plasma and proved to be simple, rapid, and sensitive for the determination of lacosamide in plasma. This method can be used in routine every day analysis of plasma samples obtained from patients who follow respective antiepileptic treatment and for the investigation of clinical and forensic cases where lacosamide is involved.
Faculty of Medicine, Department of Forensic Medicine and Toxicology, National and Kapodistrian University of Athens, Greece.
Panagiota
P
Nikolaou
Ioannis
I
Papoutsis
Chara
C
Spiliopoulou
Constantinos
C
Voudris
Sotiris
S
Athanaselis
D000081:Acetamides / Q000097:blood*; D000927:Anticonvulsants / Q000097:blood* / Q000627:therapeutic use; D002138:Calibration; D016903:Drug Monitoring / Q000379:methods*; D004827:Epilepsy / Q000188:drug therapy*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D000078334:Lacosamide; D011786:Quality Control
D000081:Acetamides; D000927:Anticonvulsants; D000078334:Lacosamide
Epilepsy; Gas chromatography; Lacosamide; Mass spectrometry; Plasma