Development of a sensitive ultra high performance liquid chromatography with tandem mass spectrometry method for the simultaneous quantification of nine active compounds in rat plasma and its application to a pharmacokinetic study after administration of Viscum coloratum extracts.
A simple, specific, and sensitive ultra high performance liquid chromatography with tandem mass spectrometry method was developed and validated for the simultaneous quantification of nine compounds including a new compound, rhamnazin-3-Ο-β-D-(6″-β-hydroxy-β-methyglutaryl)-β-D-glucoside-4'-Ο-β-D-glucoside, in rat plasma using baicalin as an internal standard. The plasma samples were pretreated and extracted by protein precipitation with 0.2% formic acid in acetonitrile. The analytes were separated on a Thermo Syncronis C18 column by gradient elution with a mobile phase consisting of acetonitrile and 0.1% aqueous formic acid at a flow rate of 0.25 mL/min. The detection of the analytes was performed on an electrospray ionization interface operating in positive-ion and multiple reaction monitoring acquisition modes. The calibration curves of these analytes showed good linearity (r > 0.99) within the test ranges. The lower limit of quantification ranged from 0.4 to 20.1 ng/mL for the analytes. The intra- and interday precision and accuracy were all within ±15%, and the recoveries were higher than 80.0%. The validated method was successfully applied to a pharmacokinetic study of the nine flavonoids after administration of the Viscum coloratum extracts by intravenous injection.
School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, China.
Rong-Hua
RH
Fan
Wei
W
Ding
Yu-Ying
YY
Ma
Hong-Li
HL
Lin
Lei
L
Men
Meng-Meng
MM
Duan
Yun-Li
YL
Zhao
Zhi-Guo
ZG
Yu
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D005419:Flavonoids / Q000008:administration & dosage / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics*; D007275:Injections, Intravenous; D008297:Male; D015394:Molecular Structure; D010936:Plant Extracts / Q000008:administration & dosage / Q000097:blood / Q000737:chemistry* / Q000493:pharmacokinetics; D051381:Rats; D017208:Rats, Wistar; D053719:Tandem Mass Spectrometry; D028181:Viscum / Q000737:chemistry*
D005419:Flavonoids; D010936:Plant Extracts
Flavonoids; Pharmacokinetics; Viscum coloratum