Overexpression of the CaTIP1-1 pepper gene in tobacco enhances resistance to osmotic stresses.
Both the gene expression and activity of water channel protein can control transmembrane water movement. We have reported the overexpression of CaTIP1-1, which caused a decrease in chilling tolerance in transgenic plants by increasing the size of the stomatal pore. CaTIP1-1 expression was strongly induced by salt and mannitol stresses in pepper (Capsicum annuum). However, its biochemical and physiological functions are still unknown in transgenic tobacco. In this study, transient expression of CaTIP1-1-GFP in tobacco suspension cells revealed that the protein was localized in the tonoplast. CaTIP1-1 overexpressed in radicle exhibited vigorous growth under high salt and mannitol treatments more than wild-type plants. The overexpression of CaTIP1-1 pepper gene in tobacco enhanced the antioxidant enzyme activities and increased transcription levels of reactive oxygen species-related gene expression under osmotic stresses. Moreover, the viability of transgenic tobacco cells was higher than the wild-type after exposure to stress. The pepper plants with silenced CaTIP1-1 in P70 decreased tolerance to salt and osmotic stresses using the detached leaf method. We concluded that the CaTIP1-1 gene plays an important role in response to osmotic stresses in tobacco.
College of Horticulture, Northwest A&F University, Yangling, Shaanxi 712100, China. yinyanxu2008@nwsuaf.edu.cn.
Yan-Xu
YX
Yin
Institute of Vegetable Crops, Jiangsu Academy of Agricultural Sciences, Nanjing, Jiangsu 210014, China. wangsbpep@163.net.
Shu-Bin
SB
Wang
College of Horticulture, Northwest A&F University, Yangling, Shaanxi 712100, China. yuanyi041ban@126.com.
Huai-Juan
HJ
Xiao
College of Horticulture, Northwest A&F University, Yangling, Shaanxi 712100, China. zhang1142154371@163.com.
Huai-Xia
HX
Zhang
College of Horticulture, Northwest A&F University, Yangling, Shaanxi 712100, China. zhangzhen030@163.com.
Zhen
Z
Zhang
College of Horticulture, Northwest A&F University, Yangling, Shaanxi 712100, China. jinghua08232014@126.com.
Hua
H
Jing
College of Horticulture, Northwest A&F University, Yangling, Shaanxi 712100, China. zyl41982@126.com.
Ying-Li
YL
Zhang
College of Horticulture, Northwest A&F University, Yangling, Shaanxi 712100, China. rugangchen@126.com.
Ru-Gang
RG
Chen
College of Horticulture, Northwest A&F University, Yangling, Shaanxi 712100, China. zhgong@nwsuaf.edu.cn.
Zhen-Hui
ZH
Gong
D000975:Antioxidants / Q000378:metabolism; D002212:Capsicum / Q000201:enzymology / Q000235:genetics* / Q000502:physiology; D002374:Catalase / Q000378:metabolism; D016923:Cell Death; D002470:Cell Survival; D004573:Electrolytes / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D020868:Gene Silencing; D017343:Genes, Plant; D008315:Malondialdehyde / Q000378:metabolism; D009997:Osmotic Pressure; D054464:Peroxiredoxins / Q000378:metabolism; D010641:Phenotype; D018515:Plant Leaves / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D030821:Plants, Genetically Modified; D021381:Protein Transport; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D036226:Seedlings / Q000254:growth & development; D013312:Stress, Physiological / Q000235:genetics*; D013347:Subcellular Fractions / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism; D014026:Nicotiana / Q000235:genetics* / Q000502:physiology*; D014867:Water
D000975:Antioxidants; D004573:Electrolytes; D010940:Plant Proteins; D012333:RNA, Messenger; D017382:Reactive Oxygen Species; D014867:Water; D008315:Malondialdehyde; D054464:Peroxiredoxins; D002374:Catalase; D013482:Superoxide Dismutase