Magnolin protects against contrast-induced nephropathy in rats via antioxidation and antiapoptosis.
BACKGROUND
Magnolin is the major active ingredient of the herb Magnolia fargesii which has anti-inflammatory and antioxidative effects. Oxidative stress and apoptosis are involved in the pathogenesis of contrast-induced nephropathy (CIN). We hypothesize that Magnolin could protect against CIN through antioxidative and antiapoptotic properties.


METHODS
To test whether Magnolin could attenuate CIN, oxidative stress and apoptosis, in vivo and in vitro, we utilized a rat model of ioversol-induced CIN and a cell model of oxidative stress in which HK2 cells were treated with H2O2. Rats were assigned to 4 groups (n = 6 per group): control group, ioversol group (ioversol-induced CIN), vehicle group (CIN rats pretreated with vehicle), and Magnolin group (CIN rats pretreated with 1 mg/kg Magnolin).


RESULTS
The results showed that magnolin ameliorated the renal tubular necrosis, apoptosis, and the deterioration of renal function (P < 0.05). Furthermore, Magnolin reduced the renal oxidative stress, suppressed caspase-3 activity, and increased Bcl-2 expression in vivo and in vitro.


CONCLUSIONS
Magnolin might protect CIN in rats through antioxidation and antiapoptosis.
Department of Nephrology, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, 600 Yishan Road, Shanghai 200233, China.
Feng
F
Wang
Department of Urology, Shanghai Jiao Tong University Affiliated First People's Hospital, 100 Haining Road, Shanghai 200080, China.
Guangyuan
G
Zhang
Department of Nephrology, The First Affiliated Hospital of Harbin Medical University, No. 23 Youzheng Street, Nangang District, Harbin 150001, China.
Yang
Y
Zhou
Department of Nephrology, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, 600 Yishan Road, Shanghai 200233, China.
Dingkun
D
Gui
Department of Nephrology, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, 600 Yishan Road, Shanghai 200233, China.
Junhui
J
Li
Florey Institute of Neuroscience and Mental Health, University of Melbourne, VIC 3010, Australia.
Tao
T
Xing
Department of Nephrology, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, 600 Yishan Road, Shanghai 200233, China.
Niansong
N
Wang
D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D003287:Contrast Media / Q000009:adverse effects*; D007674:Kidney Diseases / Q000139:chemically induced* / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D017705:Lignans / Q000494:pharmacology*; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D014283:Triiodobenzoic Acids / Q000009:adverse effects*
D003287:Contrast Media; D017705:Lignans; D014283:Triiodobenzoic Acids; C094148:magnolin; C054871:ioversol