A mouse model of atopic dermatitis.
Atopic dermatitis (AD) is a chronic or chronically relapsing, pruritic inflammatory skin disease. The incidence of AD has dramatically increased for the past three decades in industrialized countries. We established a highly efficient method to induce AD-like skin lesions using repeated epicutaneous treatments with house dust mite allergen and staphylococcal enterotoxin B (SEB). The dermatitis-induced mice showed increased serum IgE levels that were similar to human AD patients and also treatable with dexamethasone. This mouse AD model has been used in a vaccinia virus infection study. It will also be useful to study pathogenic processes of AD and to evaluate the efficacy of a drug candidate. In this chapter, we describe the detailed method that can induce AD-like skin inflammation in multiple mouse strains.
Division of Cell Biology, La Jolla Institute for Allergy and Immunology, 9420 Athena Circle, San Diego, CA, 92037, USA.
Yuko
Y
Kawakami
Toshiaki
T
Kawakami
D000818:Animals; D003876:Dermatitis, Atopic / Q000276:immunology; D040001:Dermatophagoides farinae / Q000276:immunology; D004195:Disease Models, Animal; D051379:Mice