Remote diffuse reflectance spectroscopy sensor for tissue engineering monitoring based on blind signal separation.
In this study the first results on evaluation and assessment of grafted bioengineered skin substitutes using an optical Diffuse Reflectance Spectroscopy (DRS) system with a remote optical probe are shown. The proposed system is able to detect early vascularization of skin substitutes expressing the Vascular Endothelial Growth Factor (VEGF) protein compared to normal grafts, even though devitalized skin is used to protect the grafts. Given the particularities of the biological problem, data analysis is performed using two Blind Signal Separation (BSS) methods: Principal Component Analysis (PCA) and Independent Component Analysis (ICA). These preliminary results are the first step towards point-of-care diagnostics for skin implants early assessment.
Department of Electronics Technology, Universidad Carlos III de Madrid, Leganes, Madrid 28911, Spain.
Pedro
P
Martín-Mateos
Epithelial Biomedicine Division, CIEMAT, Avenida Complutense 40, Madrid 28040, Spain and Centro de Investigación Biomédica en Red de Enfermedades Raras (CIBERER), Madrid, Spain.
Sergio
S
Crespo-Garcia
Department of Electronics Technology, Universidad Carlos III de Madrid, Leganes, Madrid 28911, Spain.
Marta
M
Ruiz-Llata
Department of Electronics Technology, Universidad Carlos III de Madrid, Leganes, Madrid 28911, Spain.
José Ramón
JR
Lopez-Fernandez
Epithelial Biomedicine Division, CIEMAT, Avenida Complutense 40, Madrid 28040, Spain and Centro de Investigación Biomédica en Red de Enfermedades Raras (CIBERER), Madrid, Spain ; Department of Bioengineering, Universidad Carlos III de Madrid, Leganes, Madrid 28911, Spain.
José Luis
JL
Jorcano
Epithelial Biomedicine Division, CIEMAT, Avenida Complutense 40, Madrid 28040, Spain and Centro de Investigación Biomédica en Red de Enfermedades Raras (CIBERER), Madrid, Spain ; Department of Bioengineering, Universidad Carlos III de Madrid, Leganes, Madrid 28911, Spain.
Marcela
M
Del Rio
Epithelial Biomedicine Division, CIEMAT, Avenida Complutense 40, Madrid 28040, Spain and Centro de Investigación Biomédica en Red de Enfermedades Raras (CIBERER), Madrid, Spain ; Department of Bioengineering, Universidad Carlos III de Madrid, Leganes, Madrid 28911, Spain.
Fernando
F
Larcher
Department of Electronics Technology, Universidad Carlos III de Madrid, Leganes, Madrid 28911, Spain.
Pablo
P
Acedo
(170.6510) Spectroscopy, tissue diagnostics; (170.6935) Tissue characterization