Longitudinal cerebral metabolic changes in pig-tailed macaques infected with the neurovirulent virus SIVsmmFGb.
Longitudinal cerebral metabolite changes in pig-tailed macaques inoculated with the simian immunodeficiency virus SIVsmmFGb were evaluated with in vivo proton MRS at 3 T. Blood sample collection, and MRS were carried out before and 2, 4, 8, 12, 16, 20, and 24 weeks after SIV inoculation. Significant reduction of N-acetylaspartate (NAA)/creatine (Cr) and choline (Cho)/Cr ratios in prefrontal gray matter (PGM) and glutamate/glutamine(Glx)/Cr ratio in striatum, and increase of myo-inositol (mI)/Cr in striatum were observed during acute SIV infection. The metabolite alterations during the SIVsmmFGb infection are largely in agreement with previous findings in other non-human primate models and HIV patients. Also, NAA/Cr in PGM and striatum and Glx/Cr in striatum are negatively correlated with the percentage of CD8+ T cells after the SIV infection, suggesting the interaction between brain metabolite and immune dysfunction. The present study complements previous studies by describing the time course of alterations of brain metabolites during SIVsmmFGb infection. The findings further demonstrate the efficacy of the SIVsmmFGb-infected macaque as a model to characterize central nervous system infection using novel neuroimaging approaches and also as a tool for exploration of novel and advanced neuroimaging techniques in HIV/AIDS studies.
Yerkes Imaging Center, Yerkes National Primate Research Center, Emory University, 954 Gatewood Rd, Atlanta, GA, 30329, USA.
Chun-Xia
CX
Li
Xiaodong
X
Zhang
Amelia
A
Komery
Yingxia
Y
Li
Hui
H
Mao
James G
JG
Herndon
Francis J
FJ
Novembre
D000818:Animals; D001224:Aspartic Acid / Q000031:analogs & derivatives / Q000378:metabolism; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology / Q000473:pathology / Q000821:virology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology / Q000473:pathology / Q000821:virology; D002794:Choline / Q000378:metabolism; D003342:Corpus Striatum / Q000378:metabolism* / Q000473:pathology / Q000821:virology; D003401:Creatine / Q000378:metabolism; D004195:Disease Models, Animal; D015658:HIV Infections / Q000276:immunology / Q000378:metabolism / Q000473:pathology / Q000821:virology; D006801:Humans; D007294:Inositol / Q000378:metabolism; D008137:Longitudinal Studies; D008254:Macaca nemestrina; D008297:Male; D017397:Prefrontal Cortex / Q000378:metabolism* / Q000473:pathology / Q000821:virology; D066244:Proton Magnetic Resonance Spectroscopy; D016097:Simian Acquired Immunodeficiency Syndrome / Q000276:immunology / Q000378:metabolism* / Q000473:pathology / Q000821:virology; D015302:Simian Immunodeficiency Virus / Q000472:pathogenicity* / Q000502:physiology; D014774:Virulence
D001224:Aspartic Acid; D007294:Inositol; C000179:N-acetylaspartate; D003401:Creatine; D002794:Choline