Biocatalysts: application and engineering for industrial purposes.
Enzymes are widely applied in various industrial applications and processes, including the food and beverage, animal feed, textile, detergent and medical industries. Enzymes screened from natural origins are often engineered before entering the market place because their native forms do not meet the requirements for industrial application. Protein engineering is concerned with the design and construction of novel enzymes with tailored functional properties, including stability, catalytic activity, reaction product inhibition and substrate specificity. Two broad approaches have been used for enzyme engineering, namely, rational design and directed evolution. The powerful and revolutionary techniques so far developed for protein engineering provide excellent opportunities for the design of industrial enzymes with specific properties and production of high-value products at lower production costs. The present review seeks to highlight the major fields of enzyme application and to provide an updated overview on previous protein engineering studies wherein natural enzymes were modified to meet the operational conditions required for industrial application.
a Laboratoire de Microorganismes et de Biomolécules , Centre de Biotechnologie de Sfax, Université de Sfax , Tunisie.
Sonia
S
Jemli
a Laboratoire de Microorganismes et de Biomolécules , Centre de Biotechnologie de Sfax, Université de Sfax , Tunisie.
Dorra
D
Ayadi-Zouari
a Laboratoire de Microorganismes et de Biomolécules , Centre de Biotechnologie de Sfax, Université de Sfax , Tunisie.
Hajer Ben
HB
Hlima
a Laboratoire de Microorganismes et de Biomolécules , Centre de Biotechnologie de Sfax, Université de Sfax , Tunisie.
Samir
S
Bejar
D055162:Biocatalysis; D057005:Bioengineering; D001709:Biotechnology; D004798:Enzymes; D007221:Industry
D004798:Enzymes
Bioengineering; directed evolution; industrial requirements; protein design; rational design; semi-rational design; tailor-made enzyme; white biotechnology