Multiplex serology for common viral infections in feral pigs (Sus scrofa) in Hawaii between 2007 and 2010.
Multiplex serology was performed for the detection of total immunoglobulin (Ig) and IgM antibodies against porcine circovirus type 2 (PCV2), porcine reproductive and respiratory syndrome virus (PRRSV), and swine influenza virus (SIV) antigens in feral swine (Sus scrofa). Serum samples were collected from the islands of Oahu (292 pigs) and Hawaii (52 pigs) between 2007 and 2010. The highest antibody prevalence was to PCV2 (63%), followed by SIV (7.8%) and PRRSV (5.8%). Antigen-specific IgM was detected at a much lower prevalence. PCR amplification and sequence analysis of PCV2 in three IgM-positive samples identified PCV2b as the only genotype. While the prevalence of PCV2 and PRRSV remained similar between 2007 and 2010, the percentage of SIV-positive samples on Oahu increased from 2% to 19%. Our results demonstrate the utility of multiplex serology for pathogen surveillance in feral pig populations.
1 School of Chemistry and Molecular Biosciences, University of Queensland, Chemistry Building 68 Cooper Rd., Brisbane, QLD 4072, Australia.
Rachel J
RJ
Stephenson
Benjamin R
BR
Trible
Yu
Y
Wang
Maureen A
MA
Kerrigan
Samuel M
SM
Goldstein
Raymond R R
RR
Rowland
D000818:Animals; D000835:Animals, Wild; D000914:Antibodies, Viral / Q000097:blood; D017925:Circovirus / Q000235:genetics / Q000276:immunology; D006254:Hawaii / Q000453:epidemiology; D007075:Immunoglobulin M / Q000097:blood; D009980:Influenza A virus / Q000276:immunology; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary*; D019316:Porcine respiratory and reproductive syndrome virus / Q000276:immunology; D012698:Serologic Tests / Q000662:veterinary; D013552:Swine; D013553:Swine Diseases / Q000097:blood / Q000453:epidemiology / Q000821:virology*; D013997:Time Factors; D014777:Virus Diseases / Q000097:blood / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology
D000914:Antibodies, Viral; D007075:Immunoglobulin M
Feral pigs; Hawaii; fluorescence microsphere immunoassay; porcine circovirus; porcine reproductive and respiratory syndrome virus; swine influenza virus