Evaluation of the influence of the antibiotic ciprofloxacin in the development of an Old World screwworm fly, Chrysomya putoria.
Chrysomya putoria (Wiedemann) (Diptera: Calliphoridae), an Old World screwworm fly, is a species with potential for maggot therapy practice and has been described in myiasis and forensic entomology studies. The objective of the present study was to assess the action of different ciprofloxacin concentrations on the growth and development of C. putoria. First instar maggots of the third generation were raised on 60 g of chicken gizzard homogenate in 65% agar diet and received ciprofloxacin chloridrate. Each concentration of the antibiotic tested (3.33 µg/mL, 6.66 µg/mL, and 13.33 µg/mL) and the control (no antibiotic) were replicated four times (40 maggots/replication). The control received distilled water instead of the antibiotic. Maggots were kept in an acclimatized chamber at 30° C during the day and 28° C at night, with 70 + 10% RH and a 14:10 L:D photoperiod. They were weighed in batches of five and stored in test tubes sealed with nylon fabric and elastic. Microsoft Excel and STAT were used for the analysis. The variation among the maggot weight means and the duration of the maggot stage, pupal stage, and time to total development (neolarvae to adult) were analyzed by Student's t-test (α= 5%). The viabilities and the normality rates were compared using ANOVA, and the expected sex ratio frequency was tested by the chisquared test (χ(2)). There was no significant difference among the four treatments regarding mean individual maggot weight, mean duration of the maggot inoculation until abandonment, the duration of the maggot and pupal stages, and the total duration of all stages. The sex ratios found in the four treatments did not differ from the expected. Only treatment 2 (6.66 µg/mL concentration of ciprofloxacin) differed significantly from the control in maggot and total viability. The antibiotic did not seem to alter C. putoria development in the postembryonic period.
Universidade Federal Rural do Rio de Janeiro, Curso de pós-graduação. Rod. BR 465, Km 7, CEP 23890-000 Seropédica, RJ, Brazil Universidade Federal do Estado do Rio de Janeiro, Rua Frei Caneca, 94, Centro, CEP 20211-040 Rio de Janeiro, RJ, Brasil adrianapedroso7@yahoo.com.br.
Adriana C P
AC
Ferraz
Universidade Federal do Estado do Rio de Janeiro, Rua Frei Caneca, 94, Centro, CEP 20211-040 Rio de Janeiro, RJ, Brasil danieledallavecchia@gmail.com.
Daniele L
DL
Dallavecchia
Universidade Federal Rural do Rio de Janeiro, Curso de pós-graduação. Rod. BR 465, Km 7, CEP 23890-000 Seropédica, RJ, Brazil Universidade Estadual do Sudoeste da Bahia, Departamento de Estudos Básicos e Instrumentais, Praça Primavera s/n, Bairro Primavera, CEP 45700-000, Itapetinga, BA, Brasil dcardoso_rj@hotmail.com.
Débora Cardoso
DC
da Silva
Universidade Federal do Estado do Rio de Janeiro, Rua Frei Caneca, 94, Centro, CEP 20211-040 Rio de Janeiro, RJ, Brasil rafa_pereirarj@hotmail.com.
Rafaela Pereira
RP
de Carvalho
Universidade Federal do Estado do Rio de Janeiro, Rua Frei Caneca, 94, Centro, CEP 20211-040 Rio de Janeiro, RJ, Brasil rgbk@baktron.com.br.
Renato Geraldo
RG
da Silva Filho
Universidade Federal do Estado do Rio de Janeiro, Rua Frei Caneca, 94, Centro, CEP 20211-040 Rio de Janeiro, RJ, Brasil valerialed@yahoo.com.br.
Valéria M
VM
Aguiar-Coelho
D000818:Animals; D000900:Anti-Bacterial Agents / Q000633:toxicity*; D002939:Ciprofloxacin / Q000633:toxicity*; D004175:Diptera / Q000187:drug effects* / Q000254:growth & development*; D005260:Female; D007814:Larva / Q000187:drug effects / Q000254:growth & development; D008297:Male; D011679:Pupa / Q000187:drug effects / Q000254:growth & development
D000900:Anti-Bacterial Agents; D002939:Ciprofloxacin
blowflies; drug; maggots; pupae