Gold nanoaggregates for probing single-living cell based on surface-enhanced Raman spectroscopy.
Gold nanoparticles are delivered into living cells by transient electroporation method to obtain intracellular surface-enhanced Raman spectroscopy (SERS). The subcellular localization of gold nanoparticles is characterized by transmission electron microscopy, and the forming large gold nanoaggregates are mostly found in the cytoplasm. The SERS detection of cells indicates that this kind of gold nanostructures induces a high signal enhancement of cellular chemical compositions, in addition to less cellular toxicity than that of silver nanoparticles. These results demonstrate that rapid incorporation of gold nanoparticles by electroporation into cells has great potential applications in the studies of cell biology and biomedicine.
Fujian Normal University, Ministry of Education and Fujian Provincial Key Laboratory for Photonics Technology, Key Laboratory of Optoelectronic Science and Technology for Medicine, Fuzhou 350007, China.
Peng
P
Lu
Fujian Normal University, Ministry of Education and Fujian Provincial Key Laboratory for Photonics Technology, Key Laboratory of Optoelectronic Science and Technology for Medicine, Fuzhou 350007, China.
Jing
J
Wang
Fujian Normal University, Ministry of Education and Fujian Provincial Key Laboratory for Photonics Technology, Key Laboratory of Optoelectronic Science and Technology for Medicine, Fuzhou 350007, China.
Jinyong
J
Lin
Fujian Normal University, Ministry of Education and Fujian Provincial Key Laboratory for Photonics Technology, Key Laboratory of Optoelectronic Science and Technology for Medicine, Fuzhou 350007, China.
Juqiang
J
Lin
Fujian Normal University, Ministry of Education and Fujian Provincial Key Laboratory for Photonics Technology, Key Laboratory of Optoelectronic Science and Technology for Medicine, Fuzhou 350007, China.
Nenrong
N
Liu
Fujian Normal University, Ministry of Education and Fujian Provincial Key Laboratory for Photonics Technology, Key Laboratory of Optoelectronic Science and Technology for Medicine, Fuzhou 350007, China.
Zufang
Z
Huang
Fujian Normal University, Ministry of Education and Fujian Provincial Key Laboratory for Photonics Technology, Key Laboratory of Optoelectronic Science and Technology for Medicine, Fuzhou 350007, China.
Buhong
B
Li
British Columbia Cancer Research Centre, Imaging Unit-Integrative Oncology Department, Vancouver, British Columbia, V5Z 1L3 Canada.
Haishan
H
Zeng
Fujian Normal University, Ministry of Education and Fujian Provincial Key Laboratory for Photonics Technology, Key Laboratory of Optoelectronic Science and Technology for Medicine, Fuzhou 350007, China.
Rong
R
Chen
D000465:Algorithms; D003593:Cytoplasm / Q000378:metabolism; D018274:Electroporation; D006046:Gold / Q000737:chemistry*; D056945:Hep G2 Cells; D006801:Humans; D007091:Image Processing, Computer-Assisted; D053768:Metal Nanoparticles / Q000737:chemistry*; D046529:Microscopy, Electron, Transmission; D036103:Nanotechnology; D012834:Silver / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet; D013059:Spectrum Analysis, Raman; D013499:Surface Properties
D012834:Silver; D006046:Gold