Binding of the PET radiotracer [¹⁸F]BF227 does not reflect the presence of alpha-synuclein aggregates in transgenic mice.
Alpha-synuclein (α-syn) aggregation is a neuropathological hallmark of many neurodegenerative diseases, collectively termed synucleinopathies. There is currently no pre-mortem diagnosis tool for these diseases. Although some compounds have been described as potential ligands for α-syn aggregates, no specific PET radiotracer of aggregated α-syn is currently available. Recently, [(18)F]BF227 has been proposed as an α-syn PET radiotracer in the absence of other specific candidates. We proposed here, for the first time, to use this radiotracer in an accelerated mouse model of synucleinopathy presenting α-syn depositions in brainstem and thalamus. Our in vivo and in vitro studies showed that [(18)F]BF227 does not bind to α-syn aggregates. These results highlight the fact that [(18)F]BF227 PET has no suitable characteristics for monitoring this experimental synucleinopathy, justifying the need to develop alternative α-syn PET radiotracers.
Elise
E
Levigoureux
Sophie
S
Lancelot
Caroline
C
Bouillot
Fabien
F
Chauveau
Mathieu
M
Verdurand
Jeremy
J
Verchere
Thierry
T
Billard
Thierry
T
Baron
CERMEP-Imaging Platform, 59 boulevard Pinel, Lyon, F-69003, France. zimmer@univ-lyon1.fr.
Luc
L
Zimmer
D000818:Animals; D001583:Benzoxazoles / Q000737:chemistry; D001933:Brain Stem / Q000000981:diagnostic imaging / Q000473:pathology; D004195:Disease Models, Animal; D019788:Fluorodeoxyglucose F18; D006801:Humans; D008279:Magnetic Resonance Imaging; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D009154:Mutation / Q000235:genetics; D049268:Positron-Emission Tomography; D011869:Radioligand Assay; D024801:Tauopathies / Q000000981:diagnostic imaging* / Q000235:genetics; D013788:Thalamus / Q000000981:diagnostic imaging / Q000473:pathology; D013844:Thiazoles / Q000737:chemistry; D051844:alpha-Synuclein / Q000235:genetics / Q000378:metabolism*
C516779:2-(2-(2-dimethylaminothiazol-5-yl)ethenyl)-6-(2-(fluoro)ethoxy)benzoxazole; D001583:Benzoxazoles; D013844:Thiazoles; D051844:alpha-Synuclein; D019788:Fluorodeoxyglucose F18