Cre/loxP-based mouse models of mast cell deficiency and mast cell-specific gene inactivation.
Over the past decades, research on in vivo functions of mast cells has largely relied on kit-mutant mouse strains. Recently, new mouse models for investigation of mast cell functions based on the Cre/loxP recombination system have been published and results in these new models challenged findings of previous studies in kit-mutant mice. Herein we describe procedures central to mast cell-specific gene inactivation and the generation of mast cell-deficient mice based on the mouse strain Mcpt5-Cre, which expresses Cre recombinase selectively in connective tissue mast cells.
Medical Faculty "Carl-Gustav Carus", Institute for Immunology, Technische Universität Dresden, Fetscherstraße 74, Dresden, 01307, Germany.
Katrin
K
Peschke
Anne
A
Dudeck
Anja
A
Rabenhorst
Karin
K
Hartmann
Axel
A
Roers
D000483:Alleles; D000818:Animals; D001665:Binding Sites; D053818:Chymases / Q000235:genetics; D003238:Connective Tissue / Q000276:immunology; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D005434:Flow Cytometry; D017353:Gene Deletion; D055879:Gene Knock-In Techniques; D020868:Gene Silencing; D005818:Genetic Engineering / Q000379:methods*; D060005:Genotyping Techniques; D019426:Integrases / Q000378:metabolism*; D008407:Mast Cells / Q000166:cytology* / Q000378:metabolism*; D051379:Mice; D016133:Polymerase Chain Reaction; D011995:Recombination, Genetic; D019076:Transgenes / Q000235:genetics
D004247:DNA; C045073:Cre recombinase; D019426:Integrases; C509227:Cma1 protein, mouse; D053818:Chymases