Structure of type II dehydroquinase from Pseudomonas aeruginosa.
Pseudomonas aeruginosa causes opportunistic infections and is resistant to most antibiotics. Ongoing efforts to generate much-needed new antibiotics include targeting enzymes that are vital for P. aeruginosa but are absent in mammals. One such enzyme, type II dehydroquinase (DHQase), catalyzes the interconversion of 3-dehydroquinate and 3-dehydroshikimate, a necessary step in the shikimate pathway. This step is vital for the proper synthesis of phenylalanine, tryptophan, tyrosine and other aromatic metabolites. The recombinant expression, purification and crystal structure of catalytically active DHQase from P. aeruginosa (PaDHQase) are presented. Cubic crystals belonging to space group F23, with unit-cell parameters a=b=c=125.39 Å, were obtained by vapor diffusion in sitting drops and the structure was refined to an R factor of 16% at 1.74 Å resolution. PaDHQase is a prototypical type II DHQase with the classical flavodoxin-like α/β topology.
Toxicology Department, School of Public Health University, University of Nebraska Medical Center, Omaha, NE 68198, USA.
Scott
S
Reiling
National School of Tropical Medicine, Baylor College of Medicine, Houston, TX 77030, USA.
Alan
A
Kelleher
National School of Tropical Medicine, Baylor College of Medicine, Houston, TX 77030, USA.
Monica M
MM
Matsumoto
National School of Tropical Medicine, Baylor College of Medicine, Houston, TX 77030, USA.
Gonteria
G
Robinson
National School of Tropical Medicine, Baylor College of Medicine, Houston, TX 77030, USA.
Oluwatoyin A
OA
Asojo
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics; D018360:Crystallography, X-Ray; D006836:Hydro-Lyases / Q000737:chemistry* / Q000235:genetics; D008969:Molecular Sequence Data; D017433:Protein Structure, Secondary; D011550:Pseudomonas aeruginosa / Q000201:enzymology* / Q000235:genetics
D001426:Bacterial Proteins; D006836:Hydro-Lyases; C020000:3-dehydroquinate dehydratase
Pseudomonas aeruginosa; shikimate pathway; type II dehydroquinase