Functional diversity of carbohydrate-active enzymes enabling a bacterium to ferment plant biomass.
Microbial metabolism of plant polysaccharides is an important part of environmental carbon cycling, human nutrition, and industrial processes based on cellulosic bioconversion. Here we demonstrate a broadly applicable method to analyze how microbes catabolize plant polysaccharides that integrates carbohydrate-active enzyme (CAZyme) assays, RNA sequencing (RNA-seq), and anaerobic growth screening. We apply this method to study how the bacterium Clostridium phytofermentans ferments plant biomass components including glucans, mannans, xylans, galactans, pectins, and arabinans. These polysaccharides are fermented with variable efficiencies, and diauxies prioritize metabolism of preferred substrates. Strand-specific RNA-seq reveals how this bacterium responds to polysaccharides by up-regulating specific groups of CAZymes, transporters, and enzymes to metabolize the constituent sugars. Fifty-six up-regulated CAZymes were purified, and their activities show most polysaccharides are degraded by multiple enzymes, often from the same family, but with divergent rates, specificities, and cellular localizations. CAZymes were then tested in combination to identify synergies between enzymes acting on the same substrate with different catalytic mechanisms. We discuss how these results advance our understanding of how microbes degrade and metabolize plant biomass.
Genoscope, CEA, DSV, IG, Évry, France; CNRS-UMR8030, Évry, France; Department of Biology, Université d'Évry Val d'Essonne, Évry, France.
Magali
M
Boutard
Genoscope, CEA, DSV, IG, Évry, France; CNRS-UMR8030, Évry, France; Department of Biology, Université d'Évry Val d'Essonne, Évry, France.
Tristan
T
Cerisy
Genoscope, CEA, DSV, IG, Évry, France; CNRS-UMR8030, Évry, France; Department of Biology, Université d'Évry Val d'Essonne, Évry, France.
Pierre-Yves
PY
Nogue
Genoscope, CEA, DSV, IG, Évry, France.
Adriana
A
Alberti
Genoscope, CEA, DSV, IG, Évry, France; CNRS-UMR8030, Évry, France.
Jean
J
Weissenbach
Genoscope, CEA, DSV, IG, Évry, France; CNRS-UMR8030, Évry, France; Department of Biology, Université d'Évry Val d'Essonne, Évry, France.
Marcel
M
Salanoubat
Genoscope, CEA, DSV, IG, Évry, France; CNRS-UMR8030, Évry, France; Department of Biology, Université d'Évry Val d'Essonne, Évry, France.
Andrew C
AC
Tolonen
D002473:Cell Wall / Q000378:metabolism; D002482:Cellulose / Q000235:genetics / Q000378:metabolism; D003001:Cloning, Molecular; D003013:Clostridium / Q000201:enzymology*; D004798:Enzymes / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D005285:Fermentation; D005947:Glucose / Q000378:metabolism; D006801:Humans; D010944:Plants / Q000378:metabolism*; D011134:Polysaccharides / Q000378:metabolism*; D017423:Sequence Analysis, RNA; D014994:Xylose / Q000235:genetics / Q000378:metabolism
D004798:Enzymes; D011134:Polysaccharides; D002482:Cellulose; D014994:Xylose; D005947:Glucose