A real-time PCR assay for the diagnosis of gastrointestinal nematode infections of small ruminants.
The diagnosis of gastrointestinal nematode infections in small ruminants is central to studying the biology and epidemiology of these parasites and underpins their control. Traditional methods of diagnosis are inaccurate, time-consuming and laborious. Here, we describe a step-by-step protocol for the molecular-based diagnosis of infections by real-time PCR.
Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Parkville, VIC, 3010, Australia.
Florian
F
Roeber
Aaron R
AR
Jex
Robin B
RB
Gasser
D000820:Animal Diseases / Q000175:diagnosis* / Q000469:parasitology*; D000818:Animals; D005767:Gastrointestinal Diseases / Q000662:veterinary*; D009348:Nematoda / Q000235:genetics*; D009349:Nematode Infections / Q000662:veterinary*; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D012418:Ruminants