DSTYK kinase domain ablation impaired the mice capabilities of learning and memory in water maze test.
DSTYK (Dual serine/threonine and tyrosine protein kinase) is a putative dual Ser/Thr and Tyr protein kinase with unique structural features. It is proposed that DSTYK may play important roles in brain because of its high expression in most brain areas. In the present study, a DSTYK knockout (KO) mouse line with the ablation of C-terminal of DSTYK including the kinase domain was generated to study the physiological function of DSTYK. The DSTYK KO mice are fertile and have no significant morphological defects revealed by Nissl staining compared with wildtype mice. Open field test and rotarod test showed there is no obvious difference in basic motor and balance capacity between the DSTYK homozygous KO mice and DSTYK heterozygous KO mice. In water maze test, however, the DSTYK homozygous KO mice show impaired capabilities of learning and memory compared with the DSTYK heterozygous KO mice.
Shanghai Key Laboratory of New Drug Design, School of Pharmacy, East China University of Science and Technology 130 Meilong Road, Shanghai, China ; Shanghai Institute of Materia Medica, Chinese Academy of Sciences Shanghai, China.
Kui
K
Li
Shanghai Key Laboratory of New Drug Design, School of Pharmacy, East China University of Science and Technology 130 Meilong Road, Shanghai, China.
Ji-Wei
JW
Liu
Shanghai Key Laboratory of New Drug Design, School of Pharmacy, East China University of Science and Technology 130 Meilong Road, Shanghai, China.
Zhi-Chuan
ZC
Zhu
Shanghai Key Laboratory of New Drug Design, School of Pharmacy, East China University of Science and Technology 130 Meilong Road, Shanghai, China.
Hong-Tao
HT
Wang
Shanghai Key Laboratory of New Drug Design, School of Pharmacy, East China University of Science and Technology 130 Meilong Road, Shanghai, China.
Yong
Y
Zu
Shanghai Key Laboratory of New Drug Design, School of Pharmacy, East China University of Science and Technology 130 Meilong Road, Shanghai, China.
Yong-Jie
YJ
Liu
Shanghai Key Laboratory of New Drug Design, School of Pharmacy, East China University of Science and Technology 130 Meilong Road, Shanghai, China.
Yan-Hong
YH
Yang
Shanghai Key Laboratory of New Drug Design, School of Pharmacy, East China University of Science and Technology 130 Meilong Road, Shanghai, China.
Zhi-Qi
ZQ
Xiong
Shanghai Institute of Materia Medica, Chinese Academy of Sciences Shanghai, China.
Xu
X
Shen
Department of Molecular and Human Genetics, Baylor College of Medicine Houston, Texas, United States of America.
Rui
R
Chen
Shanghai Key Laboratory of New Drug Design, School of Pharmacy, East China University of Science and Technology 130 Meilong Road, Shanghai, China.
Jing
J
Zheng
Shanghai Key Laboratory of New Drug Design, School of Pharmacy, East China University of Science and Technology 130 Meilong Road, Shanghai, China.
Ze-Lan
ZL
Hu
D000818:Animals; D001522:Behavior, Animal; D005838:Genotype; D007859:Learning Disabilities / Q000201:enzymology* / Q000235:genetics / Q000523:psychology; D008297:Male; D018782:Maze Learning; D008568:Memory; D008569:Memory Disorders / Q000201:enzymology* / Q000235:genetics / Q000523:psychology; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009043:Motor Activity; D010641:Phenotype; D053422:Receptor-Interacting Protein Serine-Threonine Kinases / Q000172:deficiency* / Q000235:genetics; D045442:Rotarod Performance Test
D053422:Receptor-Interacting Protein Serine-Threonine Kinases; C000599269:DSTYK protein, mouse
DSTYK; brain development; knockout mice; learning and memory