Development of a novel fluorescence probe capable of assessing the cytoplasmic entry of siderophore-based conjugates.
A novel fluorescence probe capable of assessing the cytoplasmic entry of siderophore-based conjugates was synthesized and evaluated by photochemical characterization and cell-based assays. The specific responsiveness to the cytoplasmic entry of the probe was implemented by adopting a disulfide linker, whose cleavage under the reducing conditions of the cytoplasm induced the display of a distinctive fluorescence signal.
Department of Chemistry, College of Science, Korea University, Seoul 136-701, Republic of Korea. hakkim@korea.ac.kr.
Hyeon Seok
HS
Kim
Woon Young
WY
Song
Hak Joong
HJ
Kim
D001692:Biological Transport; D003593:Cytoplasm / Q000378:metabolism*; D004220:Disulfides / Q000737:chemistry; D015195:Drug Design; D004926:Escherichia coli / Q000166:cytology; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D055668:Photochemical Processes; D016958:Pseudomonas putida / Q000166:cytology; D017262:Siderophores / Q000737:chemistry* / Q000378:metabolism*; D013050:Spectrometry, Fluorescence
D004220:Disulfides; D005456:Fluorescent Dyes; D017262:Siderophores