Characterization of homologous and heterologous interactions between viroplasm proteins P6 and P9-1 of the fijivirus southern rice black-streaked dwarf virus.
P6 of southern rice black-streaked dwarf virus (SRBSDV) is a multifunctional protein that is involved in the formation of viroplasms by interacting with P5-1. Here, we used yeast two-hybrid and bimolecular fluorescence complementation assays to show that there were homologous and heterologous interactions between SRBSDV P6 and P9-1 in yeast and plant cells. Mutational analysis showed that the N-terminal region (residues 1-93) of P6 was necessary for the interaction between P6 and P9-1. Self-interactions only occurred between the full-length P6 or P9-1. P9-1 was able to form viroplasm-like inclusion structures alone in the absence of other viral proteins.
State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, Key Laboratory of Plant Protection and Biotechnology, Ministry of Agriculture, Zhejiang Provincial Key Laboratory of Plant Virology, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, China.
Jing
J
Li
Jin
J
Xue
Heng-Mu
HM
Zhang
Jian
J
Yang
Li
L
Xie
Jian-Ping
JP
Chen
D000595:Amino Acid Sequence; D009154:Mutation; D016366:Open Reading Frames / Q000235:genetics; D012275:Oryza / Q000821:virology; D010935:Plant Diseases / Q000821:virology; D012367:RNA, Viral / Q000235:genetics; D012087:Reoviridae / Q000235:genetics*; D014764:Viral Proteins / Q000235:genetics* / Q000378:metabolism*; D014779:Virus Replication
D012367:RNA, Viral; D014764:Viral Proteins