Core bioactive components promoting blood circulation in the traditional Chinese medicine compound xueshuantong capsule (CXC) based on the relevance analysis between chemical HPLC fingerprint and in vivo biological effects.
Compound xueshuantong capsule (CXC) is an oral traditional Chinese herbal formula (CHF) comprised of Panax notoginseng (PN), Radix astragali (RA), Salvia miltiorrhizae (SM), and Radix scrophulariaceae (RS). The present investigation was designed to explore the core bioactive components promoting blood circulation in CXC using high-performance liquid chromatography (HPLC) and animal studies. CXC samples were prepared with different proportions of the 4 herbs according to a four-factor, nine-level uniform design. CXC samples were assessed with HPLC, which identified 21 components. For the animal experiments, rats were soaked in ice water during the time interval between two adrenaline hydrochloride injections to reduce blood circulation. We assessed whole-blood viscosity (WBV), erythrocyte aggregation and red corpuscle electrophoresis indices (EAI and RCEI, respectively), plasma viscosity (PV), maximum platelet aggregation rate (MPAR), activated partial thromboplastin time (APTT), and prothrombin time (PT). Based on the hypothesis that CXC sample effects varied with differences in components, we performed grey relational analysis (GRA), principal component analysis (PCA), ridge regression (RR), and radial basis function (RBF) to evaluate the contribution of each identified component. Our results indicate that panaxytriol, ginsenoside Rb1, angoroside C, protocatechualdehyde, ginsenoside Rd, and calycosin-7-O-β-D-glucoside are the core bioactive components, and that they might play different roles in the alleviation of circulation dysfunction. Panaxytriol and ginsenoside Rb1 had close relevance to red blood cell (RBC) aggregation, angoroside C was related to platelet aggregation, protocatechualdehyde was involved in intrinsic clotting activity, ginsenoside Rd affected RBC deformability and plasma proteins, and calycosin-7-O-β-D-glucoside influenced extrinsic clotting activity. This study indicates that angoroside C, calycosin-7-O-β-D-glucoside, panaxytriol, and protocatechualdehyde may have novel therapeutic uses.
Guangzhou Quality R & D Center of Traditional Chinese Medicine, Guangdong Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-sen University, Guangzhou, P.R. China.
Hong
H
Liu
Guangzhou Quality R & D Center of Traditional Chinese Medicine, Guangdong Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-sen University, Guangzhou, P.R. China.
Jie-ping
JP
Liang
Guangzhou Quality R & D Center of Traditional Chinese Medicine, Guangdong Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-sen University, Guangzhou, P.R. China.
Pei-bo
PB
Li
Guangzhou Quality R & D Center of Traditional Chinese Medicine, Guangdong Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-sen University, Guangzhou, P.R. China.
Wei
W
Peng
Guangzhou Quality R & D Center of Traditional Chinese Medicine, Guangdong Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-sen University, Guangzhou, P.R. China.
Yao-yao
YY
Peng
Guangzhou Quality R & D Center of Traditional Chinese Medicine, Guangdong Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-sen University, Guangzhou, P.R. China.
Gao-min
GM
Zhang
Guangdong Zhongsheng Pharmaceutical Co., Ltd., Dongguan, P.R. China.
Cheng-shi
CS
Xie
Guangdong Zhongsheng Pharmaceutical Co., Ltd., Dongguan, P.R. China.
Chao-feng
CF
Long
Guangzhou Quality R & D Center of Traditional Chinese Medicine, Guangdong Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-sen University, Guangzhou, P.R. China.
Wei-wei
WW
Su
D000818:Animals; D001775:Blood Circulation / Q000187:drug effects*; D001809:Blood Viscosity / Q000187:drug effects; D002214:Capsules / Q000008:administration & dosage; D002851:Chromatography, High Pressure Liquid; D003373:Coumaric Acids / Q000494:pharmacology; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000737:chemistry* / Q000494:pharmacology*; D004586:Electrophoresis; D053281:Enediynes; D004903:Erythrocyte Aggregation / Q000187:drug effects; D005233:Fatty Alcohols; D005960:Glucosides / Q000494:pharmacology*; D006801:Humans; D007529:Isoflavones / Q000494:pharmacology*; D010314:Partial Thromboplastin Time; D025341:Principal Component Analysis; D051381:Rats; D012044:Regression Analysis; D014312:Trisaccharides / Q000494:pharmacology
D002214:Capsules; D003373:Coumaric Acids; D004365:Drugs, Chinese Herbal; D053281:Enediynes; D005233:Fatty Alcohols; D005960:Glucosides; D007529:Isoflavones; C519374:Panax notoginseng extract; D014312:Trisaccharides; C526426:calycosin-7-O-beta-D-glucoside; C413578:angoroside C; C057682:panaxytriol