Streptomyces klenkii sp. nov., isolated from deep marine sediment.
A novel actinomycete strain, designated S2704(T), was isolated from a deep sediment sample, collected from the southern Black Sea coast, Turkey and characterized using a polyphasic approach. Comparison of 16S rRNA gene sequences showed that strain S2704(T) is a member of the genus Streptomyces, exhibiting highest similarity with Streptomyces specialis GW 41-1564(T) (97.1 %). Strain S2704(T) could be distinguished from all other Streptomyces species with validly published names by sequence similarity values less than 97.0 %. The isolate was found to contain LL-diaminopimelic acid as the diagnostic diamino acid. Whole cell hydrolysates were found to contain glucose, mannose and ribose. The polar lipids were identified as diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylmethylethanolamine, phosphatidylinositol, glycophospholipid, a phospholipid and two unknown glycolipids. The predominant menaquinones were identified as MK-9(H8) and MK-10(H6). The major fatty acids were found to be anteiso-C17:0, iso-C16:0 and anteiso-C15:0. The G+C content of the DNA was determined to be 72.2 mol%. On the basis of its phenotypic and molecular properties, strain S2704(T) is considered to be a novel species of the genus Streptomyces, for which the name Streptomyces klenkii sp. nov. is proposed. The type strain is S2704(T) (=DSM 42104(T) = KCTC 29202(T)).
Department of Biology, Faculty of Art and Science, Ondokuz Mayis University, Kurupelit, 55139, Samsun, Turkey.
Aysel
A
Veyisoglu
Nevzat
N
Sahin
D015373:Bacterial Typing Techniques; D001482:Base Composition; D002241:Carbohydrates / Q000032:analysis; D002473:Cell Wall / Q000737:chemistry; D016000:Cluster Analysis; D003600:Cytosol / Q000737:chemistry; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D003960:Diaminopimelic Acid / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D019015:Geologic Sediments / Q000382:microbiology*; D006017:Glycolipids / Q000032:analysis; D008855:Microscopy, Electron, Scanning; D008969:Molecular Sequence Data; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013302:Streptomyces / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D014421:Turkey; D024482:Vitamin K 2 / Q000032:analysis
D002241:Carbohydrates; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D006017:Glycolipids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; D003960:Diaminopimelic Acid