Direct stochastic optical reconstruction microscopy (dSTORM).
Single-molecule localization-based super-resolution microscopy can be performed with regular, bright, and photostable organic fluorophores. We review a concept termed direct stochastic optical reconstruction microscopy (dSTORM), which operates conventional fluorophores as photoswitches and provides an optical resolution of ~20 nm. We introduce the principle of dSTORM, illustrate experimental schemes, and discuss approaches for data analysis.
Institute of Physical & Theoretical Chemistry, Goethe University Frankfurt am Main, Max-von-Laue-Street 7, Frankfurt, 60438, Germany.
Ulrike
U
Endesfelder
Mike
M
Heilemann
D003584:Cytological Techniques / Q000379:methods*; D005456:Fluorescent Dyes; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D008856:Microscopy, Fluorescence / Q000379:methods*; D057054:Molecular Imaging / Q000379:methods*; D013194:Staining and Labeling
D005456:Fluorescent Dyes