Assessment of protein tyrosine phosphatases number 22 polymorphism prevalence among rheumatoid arthritis patients: A study on Iranian patients.
BACKGROUND
It has been proposed that Trp (620) allotype of protein tyrosine phosphatases number 22 (PTPN22) gene can intensify the susceptibility to rheumatoid arthritis (RA) and other autoimmune diseases. Thus, in this study, the prevalence of this polymorphism has been surveyed among RA patients compared with healthy persons. The samples were selected from Isfahan province (one of the most populated area of Iran).


METHODS
In this study, 100 patients (case group) and 100 healthy persons (control group) were participated voluntarily. The case group was selected from people who had referred to the rheumatology clinic of AlZahra University Hospital to follow-up their treatment and change their drugs dosage. The control group members, who were living in Isfahan province, mutually had similar age with patients. On a total, 22% of the case group was male and 75% of the control group was female. DNA was extracted from the blood sample of all cases and controls and the PTPN22 single nucleotide polymorphism (SNP) C1858> T gene polymorphism were studied using the polymerase chain reaction-restriction fragment length polymorphism method.


RESULTS
PTPN22 SNP C1858> T gene polymorphism was observed in 11 persons (11%) of the case group and 8 persons (8%) of the control group.


CONCLUSIONS
The results show that the difference was not statistically significant in Isfahan RA population (P = 0.47; OR = 1.42; 95% CI 0.55-3.69). Although, another study on Iranian population had shown that this polymorphism confers susceptibility to RA.
Division of Rheumatology, Isfahan University of Medical Sciences, Isfahan, Iran.
Mansour
M
Salesi
Division of Internal Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
Golshan Taghipour
GT
Boroujeni
Division of Genetics, Medical School, Isfahan University of Medical Sciences, Isfahan, Iran.
Mansoor
M
Salehi
Division of Rheumatology, Isfahan University of Medical Sciences, Isfahan, Iran.
Hadi
H
Karimzadeh
Isfahan; polymorphism; protein tyrosine phosphates number 22; rheumatoid arthritis