Notch2 signaling in mast cell development and distribution in the intestine.
Notch signaling controls cell-fate specification events in various types of blood cells, and it further regulates the function of particular blood cells. Recent studies have identified the role of Notch signaling as a determinant of mast cell fate from bone marrow progenitors and mast cell maturation towards mucosal type rather than connective tissue type. Furthermore, Notch2 has functional properties for immune defense against Strongyloides venezuelensis through properly distributing intestinal mast cells. The goal of this chapter is to provide the researchers with the comprehensive protocols to examine the functions of Notch signaling in mast cells both in vitro and in vivo.
Department ofHematology, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki, 305-8575, Japan.
Mamiko
M
Sakata-Yanagimoto
Shigeru
S
Chiba
D000818:Animals; D002135:Calcium-Binding Proteins; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D053818:Chymases / Q000378:metabolism; D017353:Gene Deletion; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D018014:Gene Transfer Techniques; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000494:pharmacology; D007422:Intestines / Q000276:immunology* / Q000469:parasitology; D008024:Ligands; D008297:Male; D008407:Mast Cells / Q000166:cytology* / Q000187:drug effects / Q000276:immunology; D008747:Methylcellulose / Q000737:chemistry; D023461:Myeloid Progenitor Cells / Q000166:cytology / Q000187:drug effects; D021381:Protein Transport; D051381:Rats; D051883:Receptor, Notch2 / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D012190:Retroviridae / Q000235:genetics; D015398:Signal Transduction / Q000187:drug effects; D013321:Strongyloides / Q000502:physiology
D002135:Calcium-Binding Proteins; C512925:Dlk1 protein, mouse; D036341:Intercellular Signaling Peptides and Proteins; D008024:Ligands; D051883:Receptor, Notch2; D008747:Methylcellulose; C083294:chymase 2; D053818:Chymases; C509231:Mcpt1 protein, rat