Systematic identification of the lysine succinylation in the protozoan parasite Toxoplasma gondii.
Lysine succinylation is a new posttranslational modification identified in histone proteins of Toxoplasma gondii, an obligate intracellular parasite of the phylum Apicomplexa. However, very little is known about their scope and cellular distribution. Here, using LC-MS/MS to identify parasite peptides enriched by immunopurification with succinyl lysine antibody, we produced the first lysine succinylome in this parasite. Overall, a total of 425 lysine succinylation sites that occurred on 147 succinylated proteins were identified in extracellular Toxoplasma tachyzoites, which is a proliferative stage that results in acute toxoplasmosis. With the bioinformatics analysis, it is shown that these succinylated proteins are evolutionarily conserved and involved in a wide variety of cellular functions such as metabolism and epigenetic gene regulation and exhibit diverse subcellular localizations. Moreover, we defined five types of definitively conserved succinylation site motifs, and the results imply that lysine residue of a polypeptide with lysine on the +3 position and without lysine at the -1 to +2 position is a preferred substrate of lysine succinyltransferase. In conclusion, our findings suggest that lysine succinylation in Toxoplasma involves a diverse array of cellular functions, although the succinylation occurs at a low level.
Department of Laboratory Medicine, The First Affiliated Hospital of Wenzhou Medical University , University Town, Chashan, Wenzhou, Zhejiang 325035, P. R. China.
Xiaolong
X
Li
Xin
X
Hu
Yujing
Y
Wan
Guizhen
G
Xie
Xiangzhi
X
Li
Di
D
Chen
Zhongyi
Z
Cheng
Xingling
X
Yi
Shaohui
S
Liang
Feng
F
Tan
D020816:Amino Acid Motifs / Q000235:genetics; D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites / Q000235:genetics; D015153:Blotting, Western; D002853:Chromatography, Liquid; D006657:Histones / Q000235:genetics / Q000378:metabolism*; D008239:Lysine / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D010455:Peptides / Q000378:metabolism; D011499:Protein Processing, Post-Translational; D020543:Proteome / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*; D015800:Protozoan Proteins / Q000235:genetics / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid; D013386:Succinates / Q000378:metabolism*; D053719:Tandem Mass Spectrometry; D014122:Toxoplasma / Q000235:genetics / Q000378:metabolism*
D006657:Histones; D010455:Peptides; D020543:Proteome; D015800:Protozoan Proteins; D013386:Succinates; D008239:Lysine
Toxoplasma gondii; lysine succinylation; lysine succinylation motif; succinylome; tachyzoite