Pentaplex PCR as screening assay for jellyfish species identification in food products.
Salted jellyfish, a traditional food in Asian Countries, is nowadays spreading on the Western markets. In this work, we developed a Pentaplex PCR for the identification of five edible species (Nemopilema nomurai, Rhopilema esculentum, Rhizostoma pulmo, Pelagia noctiluca, and Cotylorhiza tuberculata), which cannot be identified by a mere visual inspection in jellyfish products sold as food. A common degenerated forward primer and five specie-specific reverse primers were designed to amplify COI gene regions of different lengths. Another primer pair targeted the 28SrRNA gene and was intended as common positive reaction control. Considering the high level of degradation in the DNA extracted from acidified and salted products, the maximum length of the amplicons was set at 200 bp. The PCR was developed using 66 reference DNA samples. It gave successful amplifications in 85.4% of 48 ready to eat products (REs) and in 60% of 30 classical salted products (CPs) collected on the market.
FishLab, Department of Veterinary Sciences, University of Pisa , Via delle Piagge 2, 56124 Pisa, Italy.
Andrea
A
Armani
Alice
A
Giusti
Lorenzo
L
Castigliego
Aurelio
A
Rossi
Lara
L
Tinacci
Daniela
D
Gianfaldoni
Alessandra
A
Guidi
D000818:Animals; D017931:DNA Primers / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods*; D012339:RNA, Ribosomal, 28S / Q000235:genetics; D037401:Scyphozoa / Q000145:classification / Q000235:genetics*; D017747:Seafood / Q000032:analysis*
D017931:DNA Primers; D012339:RNA, Ribosomal, 28S
COI gene; jellyfish; multiplex PCR; seafood mislabeling; species identification