A multiplex chemiluminescent biosensor for type B-fumonisins and aflatoxin B1 quantitative detection in maize flour.
A multiplex chemiluminescent biosensor for simple, rapid and ultrasensitive on-site quantification of aflatoxin B1 and type B-fumonisins in maize samples has been developed. The biosensor integrates a multiplex indirect competitive lateral flow immunoassay (LFIA) based on enzyme-catalyzed chemiluminescence detection and a highly sensitive portable charge-coupled device (CCD) camera, employed in a lensless "contact" imaging configuration. The developed assay requires a simple extraction of the analytes from maize flour samples followed by their detection with a 30 min assay time. The use of chemiluminescence detection allowed accurate and objective analytes quantification, enabling simultaneous detection of type B-fumonisins and aflatoxin B1 down to 6 μg kg(-1) and 1.5 μg kg(-1), respectively, thus fulfilling the standards imposed by the legislation of European Union. Maize flour samples spiked with both analytes were subjected to multiplex analysis obtaining recoveries ranging from 80 to 115% and the coefficient of variation below 20%. Finally, analysis of naturally contaminated maize samples resulted in a good agreement between CL-LFIA and a validated confirmatory HPLC-UV and commercial ELISA kit, obtaining recoveries in the range 88-120%. The proposed CL-LFIA protocol is rapid, inexpensive, easy-to-use, and fit for the purpose of rapid screening of mycotoxins in maize flour.
Department of Chemistry "G. Ciamician", University of Bologna, via Selmi 2, 40126 Bologna, Italy. mara.mirasoli@unibo.it.
Martina
M
Zangheri
Fabio
F
Di Nardo
Laura
L
Anfossi
Cristina
C
Giovannoli
Claudio
C
Baggiani
Aldo
A
Roda
Mara
M
Mirasoli
D016604:Aflatoxin B1 / Q000032:analysis*; D015374:Biosensing Techniques; D005433:Flour / Q000032:analysis*; D037341:Fumonisins / Q000032:analysis*; D049449:Luminescence; D003313:Zea mays / Q000737:chemistry*
D037341:Fumonisins; D016604:Aflatoxin B1