Evaluation of the Aspergillus Western blot IgG kit for diagnosis of chronic aspergillosis.
Immunoprecipitin detection (IPD) is the current reference confirmatory technique for anti-Aspergillus antibody detection; however, the lack of standardization is a critical drawback of this assay. In this study, we evaluated the performance of the Aspergillus Western blot (Asp-WB) IgG kit (LDBio Diagnostics, Lyon, France), a recently commercialized immunoblot assay for the diagnosis of various clinical presentations of chronic aspergillosis. Three hundred eight serum samples from 158 patients with aspergillosis sensu lato (s.l.) were analyzed. More specifically, 267 serum samples were derived from patients with Aspergillus disease, including 89 cases of chronic pulmonary aspergillosis, 10 of aspergilloma, and 32 of allergic bronchopulmonary aspergillosis, while 41 samples were from patients with Aspergillus colonization, including 15 cystic fibrosis (CF) and 12 non-CF patients. For blood donor controls, the Asp-WB specificity was 94%, while the kit displayed a sensitivity for the aspergillosis s.l. diagnosis of 88.6%, with a diagnostic odds ratio (DOR) of 119 (95% confidence interval [CI], 57 to 251). The DOR values were 185.22 (95% CI,78.79 to 435.45) and 43.74 (95% CI, 15.65 to 122.20) for the diagnosis of Aspergillus disease and Aspergillus colonization, respectively. Among the patients, the sensitivities of the Asp-WB in the diagnosis of Aspergillus colonization were 100% and 41.7% in CF and non-CF patients, respectively. The Asp-WB yielded fewer false-negative results than did IPD. In conclusion, the Asp-WB kit performed well for the diagnosis of various clinical presentations of aspergillosis in nonimmunocompromised patients, with an enhanced standardization and a higher sensitivity than with IPD, which is the current reference method.
Aix Marseille University, IP-TPT UMR MD3, Marseille, France.
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A
Oliva
Service des Agents Infectieux et Hygiène, CHU de Saint-Etienne, Saint-Etienne, France Université Jean Monnet, EA 3064-GIMAP, Saint-Etienne, France.
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P
Flori
Sorbonne Universités, UPMC Univ. Paris 06, CR7, Centre d'Immunologie et des Maladies Infectieuses (CIMI-Paris), Paris, France INSERM, U1135, CIMI-Paris, Paris, France CNRS, ERL 8255, CIMI-Paris, Paris, France AP-HP, Hôpital St Antoine, Service de Parasitologie-Mycologie, Paris, France.
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C
Hennequin
Pediatric Pulmonology Unit and Cystic Fibrosis Centre, APHM, CHU Timone, Marseille, France.
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JC
Dubus
Department of Pulmonary Disease and Lung Transplantation, Competence Centre for Rare Pulmonary Diseases, APHM North Hospital, Aix Marseille University, Marseille, France.
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M
Reynaud-Gaubert
Clinique des Bronches, Allergie et Sommeil, Hôpital Nord et INSERM U1067 CNRS UMR1067, Marseille, France.
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D
Charpin
Service de Pneumologie et d'Oncologie Thoracique, CHU de Saint-Etienne, Saint-Etienne, France Université Jean Monnet, EA 4624-LINA, Saint-Etienne, France.
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JM
Vergnon
Service de Pneumologie et d'Oncologie Thoracique, CHU de Saint-Etienne, Saint-Etienne, France.
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P
Gay
Service des Agents Infectieux et Hygiène, CHU de Saint-Etienne, Saint-Etienne, France.
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A
Colly
Aix Marseille University, IP-TPT UMR MD3, Marseille, France Parasitology-Mycology, APHM, CHU Timone, Marseille, France.
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R
Piarroux
Parasitologie-Mycologie, Département des Agents Infectieux, CHU Grenoble and Université Grenoble Alpes, Grenoble, France.
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H
Pelloux
Aix Marseille University, IP-TPT UMR MD3, Marseille, France Parasitology-Mycology, APHM, CHU Timone, Marseille, France stephane.ranque@ap-hm.fr.
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http://orcid.org/0000-0003-3293-5276
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Ranque
D000908:Antibodies, Fungal / Q000276:immunology*; D001228:Aspergillosis / Q000175:diagnosis* / Q000276:immunology*; D001230:Aspergillus / Q000276:immunology*; D015153:Blotting, Western; D016022:Case-Control Studies; D002908:Chronic Disease; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology*; D011933:Reagent Kits, Diagnostic; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D000908:Antibodies, Fungal; D007074:Immunoglobulin G; D011933:Reagent Kits, Diagnostic