Protective effects of sesaminol on BEAS-2B cells impaired by cigarette smoke extract.
The aim of this study is to investigate protective effects of sesaminol on the human bronchial epithelial (BEAS-2B) cell line against oxidative damage of cigarette smoke extract (CSE). BEAS-2B cells were pre-incubated with sesaminol for 12 h and then treated with various concentrations of CSE for 24 h. After that proliferation ability, levels of reactive oxygen species (ROS) and lactate dehydrogenase (LDH), cell apoptosis, activities of catalase (CAT) and superoxide dismutase (SOD), and mRNA levels of IL-8 and IL-6 were measured. The results showed that sesaminol significantly improved BEAS-2B cell viability, reduced the production of ROS and LDH of cells, inhibited cell apoptosis and increased CAT and SOD activities in CSE-treated cells. Sesaminol also inhibited the expression of IL-8 and IL-6 mRNA following CSE exposure. In conclusion, sesaminol may protect BEAS-2B cells against CSE-induced oxidative damage.
State Key Laboratory of Analytical Chemistry for Life Science and Jiangsu Key Laboratory of Molecular Medicine, Nanjing University Medical School, 22 Hankou Road, Nanjing, 210093, China.
Ping
P
Dong
Xiao
X
Fu
Xiang
X
Wang
Wen-mei
WM
Wang
Wen-ming
WM
Cao
Wei-yun
WY
Zhang
D017209:Apoptosis / Q000187:drug effects* / Q000502:physiology; D001980:Bronchi / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D002460:Cell Line; D019610:Cytoprotection / Q000187:drug effects / Q000502:physiology*; D004149:Dioxoles / Q000008:administration & dosage*; D004305:Dose-Response Relationship, Drug; D004847:Epithelial Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D005663:Furans / Q000008:administration & dosage*; D006801:Humans; D018384:Oxidative Stress / Q000187:drug effects / Q000502:physiology; D017382:Reactive Oxygen Species / Q000378:metabolism; D013638:Tars / Q000633:toxicity*
D004149:Dioxoles; D005663:Furans; D017382:Reactive Oxygen Species; D013638:Tars; C024746:tobacco tar; C066016:sesaminol