An open tubular ion chromatograph.
We describe an open tubular ion chromatograph (OTIC) that uses anion exchange latex coated 5 μm radius silica and 9.8 μm radius poly(methyl methacrylate) tubes and automated time/pressure based hydrodynamic injection for pL-nL scale injections. It is routinely possible to generate 50,000 plates or more (up to 150,000 plates/m, columns between 0.3 and 0.8 m have been used), and as such, fast separations are possible, comparable to or in some cases better than the current practice of IC. With an optimized admittance detector, nonsuppressed detection permits LODs of submicromolar to double digit micromolar for a variety of analytes. However, large volume injections are possible and can significantly improve on this. A variety of eluents, the use of organic modifiers, and variations of eluent pH can be used to tailor a given separation. The approach is discussed in the context of extraterrestrial exploration, especially Mars, where the existence of large amounts of perchlorate in the soil needs to be confirmed. These columns can survive drying and freezing, and small footprint, low power consumption, and simplicity make OTIC a good candidate for such a mission.
Department of Chemistry and Biochemistry, The University of Texas at Arlington , Arlington, Texas 76019-0065, United States.
Bingcheng
B
Yang
Min
M
Zhang
Tinakorn
T
Kanyanee
Brian N
BN
Stamos
Purnendu K
PK
Dasgupta
D000837:Anion Exchange Resins / Q000737:chemistry*; D002852:Chromatography, Ion Exchange; D057446:Hydrodynamics; D006863:Hydrogen-Ion Concentration; D019904:Polymethyl Methacrylate / Q000737:chemistry*; D011312:Pressure; D012822:Silicon Dioxide / Q000737:chemistry*; D013696:Temperature; D013997:Time Factors
D000837:Anion Exchange Resins; D012822:Silicon Dioxide; D019904:Polymethyl Methacrylate