Biocompatible flavone-based fluorogenic probes for quick wash-free mitochondrial imaging in living cells.
Mitochondria, vital organelles existing in almost all eukaryotic cells, play a crucial role in energy metabolism and apoptosis of aerobic organisms. In this work, we report two new flavone-based fluorescent probes, MC-Mito1 and MC-Mito2, for monitoring mitochondria in living cells. These two probes exhibit remarkably low toxicity, good cell permeability, and high specificity; these probes complement the existing library of mitochondrial imaging agents. The new dyes give nearly no background fluorescence, and their application does not require tedious postwashing after cell staining. The appreciable tolerance of MC-Mito2 encourages a broader range of biological applications for understanding the cell degeneration and apoptosis mechanism.
Department of Chemistry and Maurice Morton Institute of Polymer Science, ‡Department of Biomedical Engineering, and §Department of Biology, The University of Akron , Akron, Ohio 44325, United States.
Bin
B
Liu
Mickey
M
Shah
Ge
G
Zhang
Qin
Q
Liu
Yi
Y
Pang
D017209:Apoptosis / Q000235:genetics; D001672:Biocompatible Materials / Q000737:chemistry; D002463:Cell Membrane Permeability / Q000187:drug effects; D047309:Flavones / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000008:administration & dosage / Q000737:chemistry; D006801:Humans; D008928:Mitochondria / Q000648:ultrastructure*; D057054:Molecular Imaging
D001672:Biocompatible Materials; D047309:Flavones; D005456:Fluorescent Dyes; C043562:flavone
biocompatibility; flavone; fluorescence; imaging agents; mitochondria; wash-free