Changes in protein expression of pacific oyster Crassostrea gigas exposed in situ to urban sewage.
The composition and concentration of substances in urban effluents are complex and difficult to measure. These contaminants elicit biological responses in the exposed organisms. Proteomic analysis is a powerful tool in environmental toxicology by evidencing alterations in protein expression due to exposure to contaminants and by providing a useful framework for the development of new potential biomarkers. The aim of this study was to determine changes in protein expression signatures (PES) in the digestive gland of oysters Crassostrea gigas transplanted to two farming areas (LIS and RIB) and to one area contaminated by sanitary sewage (BUC) after 14 days of exposure. This species is one of the most cultivated molluscs in the world. The identified proteins are related to the cytoskeleton (CKAP5 and ACT2), ubiquitination pathway conjugation (UBE3C), G protein-coupled receptor and signal transduction (SVEP1), and cell cycle/division (CCNB3). CKAP5 showed higher expression in oysters kept at BUC in comparison with those kept at the farming areas, while ACT2, UBE3C, SVEP1, and CCNB3 were suppressed. The results suggest that these changes might lead to DNA damage, apoptosis, and interference with the immune system in oyster C. gigas exposed to sewage and give initial information on PES of C. gigas exposed to sanitary sewage, which can subsequently be useful in the development of more sensitive tools for biomonitoring coastal areas, particularly those devoted mainly to oyster farming activities.
Laboratory for Biomarkers of Aquatic Contamination and Immunochemistry, Federal University Santa Catarina, Florianópolis, Brazil.
Fabrício
F
Flores-Nunes
CIMA, Faculty of Science and Technology, University of Algarve, Campus de Gambelas, 8005-139, Faro, Portugal.
Tânia
T
Gomes
CIMA, Faculty of Science and Technology, University of Algarve, Campus de Gambelas, 8005-139, Faro, Portugal.
Rui
R
Company
Laboratory for Biomarkers of Aquatic Contamination and Immunochemistry, Federal University Santa Catarina, Florianópolis, Brazil.
Roberta R M
RR
Moraes
Laboratory of Marine Organic Chemistry, Oceanographic Institute, University of São Paulo, São Paulo, Brazil.
Silvio T
ST
Sasaki
Laboratory of Marine Organic Chemistry, Oceanographic Institute, University of São Paulo, São Paulo, Brazil.
Satie
S
Taniguchi
Laboratory of Marine Organic Chemistry, Oceanographic Institute, University of São Paulo, São Paulo, Brazil.
Márcia C
MC
Bicego
Laboratory of Marine Molluscs, Federal University of Santa Catarina, Florianópolis, Brazil.
Cláudio M R
CM
Melo
Laboratory for Biomarkers of Aquatic Contamination and Immunochemistry, Federal University Santa Catarina, Florianópolis, Brazil.
Afonso C D
AC
Bainy
CIMA, Faculty of Science and Technology, University of Algarve, Campus de Gambelas, 8005-139, Faro, Portugal. mbebian@ualg.pt.
Maria J
MJ
Bebianno
D000818:Animals; D002947:Cities; D049896:Crassostrea / Q000187:drug effects* / Q000378:metabolism*; D004784:Environmental Monitoring; D004785:Environmental Pollutants / Q000633:toxicity*; D011506:Proteins / Q000378:metabolism*; D012722:Sewage / Q000009:adverse effects*; D059467:Transcriptome / Q000187:drug effects*
D004785:Environmental Pollutants; D011506:Proteins; D012722:Sewage
Crassostrea gigas; Proteomic analysis; Two-dimensional gel electrophoresis; Urban sewage