Surface plasmon-enhanced nanoscopy of intracellular cytoskeletal actin filaments using random nanodot arrays.
The feasibility of super-resolution microscopy has been investigated based on random localization of surface plasmon using blocked random nanodot arrays. The resolution is mainly determined by the size of localized fields in the range of 100-150 nm. The concept was validated by imaging FITC-conjugated phalloidin that binds to cellular actin filaments. The experimental results confirm improved resolution in reconstructed images. Effect of far-field registration on image reconstruction was also analyzed. Correlation between reconstructed images was maintained to be above 81% after registration. Nanodot arrays are synthesized by temperature-annealing without sophisticated lithography and thus can be mass-produced in an extremely large substrate. The results suggest a super-resolution imaging technique that can be accessible and available in large amounts.
Youngjin
Y
Oh
Taehwang
T
Son
Sook Young
SY
Kim
Wonju
W
Lee
Heejin
H
Yang
Jong-Ryul
JR
Choi
Jeon-Soo
JS
Shin
Donghyun
D
Kim
D008841:Actin Cytoskeleton / Q000378:metabolism*; D000818:Animals; D002460:Cell Line; D007091:Image Processing, Computer-Assisted; D042541:Intracellular Space / Q000378:metabolism*; D051379:Mice; D008856:Microscopy, Fluorescence; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D009716:Numerical Analysis, Computer-Assisted; D020349:Surface Plasmon Resonance / Q000379:methods*