The effects of germacrone on lipopolysaccharide-induced acute lung injury in neonatal rats.
Germacrone is one of the main bioactive components in the traditional Chinese medicine Rhizoma curcuma and has been shown to possess an anti-inﬂammatory activity. Our present study aimed to investigate the protective effects of germacrone on lipopolysaccharide (LPS)-induced acute lung injury in neonatal rats. Results showed that germacrone treatment significantly decreased the expression of pro-inflammatory cytokines IL-6 and TNF-α. Meanwhile, the expression of anti-inflammatory mediators TGF-β1 and IL-10 was obviously increased following germacrone administration. The LPS-induced pathological changes in neonatal rats were also attenuated by germacrone treatment. In vitro, MTT and EdU incorporation assay indicated that germacrone administration significantly increased the A549 cell viabilities in a dose-dependent manner. Besides, flow cytometry and TUNEL analysis showed that the cell apoptosis rate was significantly reduced in a concentration-dependent manner after germacrone injection. At the molecular level, we found that germacrone treatment promoted the expression of claudin-4 both in vivo and in vitro as shown by real time PCR and western blot. Collectively, our study demonstrated that germacrone protected neonatal rats against LPS-induced ALI partially by modulation of claudin-4.
Xi&apos;an Children&apos;s Hospital Department of Integrated TCM & Western Medicine Xi&apos;an China.
J-F
JF
An
First Affiliated Hospital of Xi&apos;an Jiaotong University Department of Traditional Chinese Medicine Xi&apos;an China.
Y
Y
Sun
Shanghai Jiao Tong University Center for Reproductive Medicine, Renji Hospital, School of Medicine Shanghai China.
Q-L
QL
Zhang
Xi&apos;an Hospital of Traditional Chinese Medicine Department of Respiration Xi&apos;an China.
F-L
FL
Zhang
Xi&apos;an Children&apos;s Hospital Department of Neonatology Xi&apos;an China zjuanlijl&commat;163.com.
J-L
JL
Zhang
D055371:Acute Lung Injury / Q000139:chemically induced* / Q000188:drug therapy* / Q000276:immunology / Q000473:pathology; D000818:Animals; D000831:Animals, Newborn; D000893:Anti-Inflammatory Agents / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*; D017209:Apoptosis / Q000187:drug effects; D002460:Cell Line; D062506:Claudin-4 / Q000235:genetics; D030024:Curcuma / Q000737:chemistry; D006801:Humans; D016753:Interleukin-10 / Q000276:immunology; D015850:Interleukin-6 / Q000276:immunology; D008070:Lipopolysaccharides; D008168:Lung / Q000187:drug effects / Q000276:immunology / Q000473:pathology*; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D017208:Rats, Wistar; D045788:Sesquiterpenes, Germacrane / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*; D014409:Tumor Necrosis Factor-alpha / Q000276:immunology; D015854:Up-Regulation / Q000187:drug effects
D000893:Anti-Inflammatory Agents; D062506:Claudin-4; D015850:Interleukin-6; D008070:Lipopolysaccharides; D012333:RNA, Messenger; D045788:Sesquiterpenes, Germacrane; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10; C048393:germacrone