Elevated PD-1 expression and decreased telomerase activity in memory T cells of patients with symptomatic Herpes Zoster infection.
We investigated PD-1 levels on VZV-specific CD8+ T-cells of patients with zoster and the effect of PD-1 on the telomerase activity. CD3, CD8, CD137 and PD-1 expressions were analyzed on PBMCs from 9 symptomatic and 5 asymptomatic individuals. The effect of PD-1 blockade at the time of stimulation on the telomerase activity of non-senescent CD57-CD45RO+CD8+CD3+ memory T-cells was evaluated. PD-1 was elevated on CD8+ T-cells in patients. The frequency of PD-1+ and CD137- cells in total CD3+CD8+ T cells of patients was elevated compared to controls. Telomerase activity of non-senescent memory T-cells was lower than that of controls. Blockade of PD-1 at the time of stimulation increased telomerase activity of non-senescent memory T-cells, accompanied by increased CD137 expression. Low telomerase activity of the patients with reactivated zoster could be partially overcome by blocking PD-1 pathway.
Shiraz University of Medical Sciences Student Research Committee, School of Medicine Shiraz Iran.
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Z
Zangeneh
Shiraz University of Medical Sciences Memory T cell Laboratory, Department of Immunology, School of Medicine Shiraz Iran.
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H
Golmoghaddam
Shiraz University of Medical Sciences Department of Dermatology, School of Medicine Shiraz Iran.
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M
Emad
Shiraz University of Medical Sciences Shiraz Institute for Cancer Research, School of Medicine Shiraz Iran.
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N
Erfani
Shiraz University of Medical Sciences Memory T cell Laboratory, Department of Immunology, School of Medicine Shiraz Iran mdoroud&commat;sums.ac.ir.
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Doroudchi
D000328:Adult; D000368:Aged; D018414:CD8-Positive T-Lymphocytes / Q000201:enzymology / Q000276:immunology / Q000473:pathology*; D004789:Enzyme Activation / Q000187:drug effects; D005260:Female; D006562:Herpes Zoster / Q000201:enzymology / Q000276:immunology / Q000473:pathology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D010455:Peptides / Q000494:pharmacology; D061026:Programmed Cell Death 1 Receptor / Q000032:analysis* / Q000037:antagonists & inhibitors / Q000276:immunology; D019098:Telomerase / Q000276:immunology* / Q000378:metabolism
C105992:PDCD1 protein, human; D010455:Peptides; D061026:Programmed Cell Death 1 Receptor; D019098:Telomerase