Hydrogel design of experiments methodology to optimize hydrogel for iPSC-NPC culture.
Bioactive signals can be incorporated in hydrogels to direct encapsulated cell behavior. Design of experiments methodology methodically varies the signals systematically to determine the individual and combinatorial effects of each factor on cell activity. Using this approach enables the optimization of three ligands concentrations (RGD, YIGSR, IKVAV) for the survival and differentiation of neural progenitor cells.
Biomedical Engineering Department, University of California, Los Angeles, CA, USA.
Jonathan
J
Lam
S Thomas
ST
Carmichael
William E
WE
Lowry
Tatiana
T
Segura
D000595:Amino Acid Sequence; D002448:Cell Adhesion / Q000187:drug effects; D018929:Cell Culture Techniques / Q000379:methods*; D006801:Humans; D006820:Hyaluronic Acid / Q000494:pharmacology; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000494:pharmacology*; D057026:Induced Pluripotent Stem Cells / Q000166:cytology* / Q000187:drug effects; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D058953:Neural Stem Cells / Q000166:cytology* / Q000187:drug effects; D010455:Peptides / Q000737:chemistry / Q000494:pharmacology
D010455:Peptides; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D006820:Hyaluronic Acid
design of experiments; hyaluronic acid; hydrogel; induced pluripotent stem cells; neurons