Cryopreservation of human pluripotent stem cells: a general protocol.
Cryopreservation is an essential technique to preserve stem cells, semipermanently sustaining their potentials. There are two main approaches of cryopreservation for human pluripotent stem cells (hPSCs). The first is the vitrification, which involves instantaneous freeze and thaw of hPSCs. The second is the conventional slow-cooling method and a rapid thaw. Both cryopreservation protocols have been standardized and optimized to yield high survivability of hPSCs.
Department of Embryonic Stem Cell Research, Institute for Frontier Medical Sciences, Kyoto University, 53 Kawaharacho, Shogoin, Sakyo-Ku, Kyoto, 606-8507, Japan.
Takamichi
T
Miyazaki
Hirofumi
H
Suemori
D002470:Cell Survival; D015925:Cryopreservation / Q000379:methods*; D003451:Cryoprotective Agents / Q000737:chemistry; D006801:Humans; D039904:Pluripotent Stem Cells / Q000166:cytology*; D058989:Vitrification
D003451:Cryoprotective Agents