The selected reaction monitoring/multiple reaction monitoring-based mass spectrometry approach for the accurate quantitation of proteins: clinical applications in the cardiovascular diseases.
Selected reaction monitoring, also known as multiple reaction monitoring, is a powerful targeted mass spectrometry approach for a confident quantitation of proteins/peptides in complex biological samples. In recent years, its optimization and application have become pivotal and of great interest in clinical research to derive useful outcomes for patient care. Thus, selected reaction monitoring/multiple reaction monitoring is now used as a highly sensitive and selective method for the evaluation of protein abundances and biomarker verification with potential applications in medical screening. This review describes technical aspects for the development of a robust multiplex assay and discussing its recent applications in cardiovascular proteomics: verification of promising disease candidates to select only the highest quality peptides/proteins for a preclinical validation, as well as quantitation of protein isoforms and post-translational modifications.
Laboratory of Cell Biology and Biochemistry of Atherothrombosis, Unit of Proteomics, Centro Cardiologico Monzino IRCCS, Via Parea 4, 20138 Milan, Italy.
Erica
E
Gianazza
Elena
E
Tremoli
Cristina
C
Banfi
D015415:Biomarkers / Q000032:analysis; D035843:Biomedical Research; D002318:Cardiovascular Diseases / Q000175:diagnosis / Q000378:metabolism*; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods; D010455:Peptides / Q000032:analysis; D011506:Proteins / Q000032:analysis*; D040901:Proteomics
D015415:Biomarkers; D010455:Peptides; D011506:Proteins
biomarkers; cardiovascular diseases; endogenous proteins; mass spectrometry; multiple reaction monitoring; post-translational modifications; protein isoforms; selected reaction monitoring; targeted proteomics