Polo kinase regulates the localization and activity of the chromosomal passenger complex in meiosis and mitosis in Drosophila melanogaster.
Cell cycle progression is regulated by members of the cyclin-dependent kinase (CDK), Polo and Aurora families of protein kinases. The levels of expression and localization of the key regulatory kinases are themselves subject to very tight control. There is increasing evidence that crosstalk between the mitotic kinases provides for an additional level of regulation. We have previously shown that Aurora B activates Polo kinase at the centromere in mitosis, and that the interaction between Polo and the chromosomal passenger complex (CPC) component INCENP is essential in this activation. In this report, we show that Polo kinase is required for the correct localization and activity of the CPC in meiosis and mitosis. Study of the phenotype of different polo allele combinations compared to the effect of chemical inhibition revealed significant differences in the localization and activity of the CPC in diploid tissues. Our results shed new light on the mechanisms that control the activity of Aurora B in meiosis and mitosis.
The Wellcome Trust Centre for Cell Biology, Institute of Cell Biology, University of Edinburgh, Edinburgh EH9 3JR, UK mar.carmena@ed.ac.uk.
Mar
M
Carmena
Centre National de la Recherche Scientifique, Aix-Marseille Université, CNRS UMR 7257, AFMB, 163 Avenue de Luminy, 13288 Marseille, France.
Miguel Ortiz
MO
Lombardia
The Wellcome Trust Centre for Cell Biology, Institute of Cell Biology, University of Edinburgh, Edinburgh EH9 3JR, UK.
Hiromi
H
Ogawa
The Wellcome Trust Centre for Cell Biology, Institute of Cell Biology, University of Edinburgh, Edinburgh EH9 3JR, UK bill.earnshaw@ed.ac.uk.
William C
WC
Earnshaw
D000483:Alleles; D000595:Amino Acid Sequence; D000818:Animals; D064107:Aurora Kinase B / Q000378:metabolism; D020134:Catalytic Domain; D002868:Chromosomal Proteins, Non-Histone / Q000378:metabolism*; D004171:Diploidy; D029721:Drosophila Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000235:genetics / Q000378:metabolism*; D008540:Meiosis; D008938:Mitosis; D008969:Molecular Sequence Data; D009154:Mutation; D017346:Protein Serine-Threonine Kinases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D021381:Protein Transport
D002868:Chromosomal Proteins, Non-Histone; D029721:Drosophila Proteins; C512039:Incenp protein, Drosophila; C072004:polo protein, Drosophila; C576957:AurB protein, Drosophila; D064107:Aurora Kinase B; D017346:Protein Serine-Threonine Kinases
Aurora kinases; Drosophila; Polo-like kinases; chromosomal passenger complex; meiosis; mitosis