Selective augmentation of stem cell populations in structural fat grafts for maxillofacial surgery.
Structural fat grafting utilizes the centrifugation of liposuction aspirates to create a graded density of adipose tissue. This study was performed to qualitatively investigate the effects of centrifugation on stem cells present in adipose tissue. Liposuction aspirates were obtained from healthy donors and either not centrifuged or centrifuged at 1,800 rpm for 3 minutes. The obtained fat volumes were divided into three layers and then analyzed. The results demonstrate that centrifugation induces a different distribution of stem cells in the three layers. The high-density layer displays the highest expression of mesenchymal stem cell and endothelial markers. The low-density layer exhibits an enrichment of multipotent stem cells. We conclude that appropriate centrifugation concentrates stem cells. This finding may influence the clinical practice of liposuction aspirate centrifugation and enhance graft uptake.
Unit of Cranio Maxillo Facial Surgery, Center for Craniofacial Deformities and Orbital Surgery - Reference Center for Rare Disease, Sant'Anna Hospital and University, Cona, Ferrara, Italy.
Luigi
L
Clauser
Department of Biomedical Sciences, University of Padova, Padova, Italy.
Letizia
L
Ferroni
Department of Biomedical Sciences, University of Padova, Padova, Italy.
Chiara
C
Gardin
Unit of Cranio Maxillo Facial Surgery, Center for Craniofacial Deformities and Orbital Surgery - Reference Center for Rare Disease, Sant'Anna Hospital and University, Cona, Ferrara, Italy.
Riccardo
R
Tieghi
Unit of Cranio Maxillo Facial Surgery, Center for Craniofacial Deformities and Orbital Surgery - Reference Center for Rare Disease, Sant'Anna Hospital and University, Cona, Ferrara, Italy.
Manlio
M
Galiè
Unit of Cranio Maxillo Facial Surgery, Center for Craniofacial Deformities and Orbital Surgery - Reference Center for Rare Disease, Sant'Anna Hospital and University, Cona, Ferrara, Italy.
Giovanni
G
Elia
Department of Medical, Oral, and Biotechnological Sciences, University of Chieti-Pescara, Chieti, Italy.
Adriano
A
Piattelli
Department of Morphology, Surgery and Experimental Medicine, Section of Pathology Oncology and Experimental Biology, University of Ferrara, Ferrara, Italy.
Paolo
P
Pinton
Department of Neurosciences, University of Padova, Padova, Italy.
Eriberto
E
Bressan
Department of Biomedical Sciences, University of Padova, Padova, Italy.
Barbara
B
Zavan
D000273:Adipose Tissue / Q000637:transplantation*; D000328:Adult; D002498:Centrifugation; D005260:Female; D006801:Humans; D015187:Lipectomy / Q000379:methods; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D013515:Surgery, Oral / Q000379:methods*; D054457:Tissue Scaffolds; D055815:Young Adult