Culturing protocols for human multipotent adult stem cells.
Culture procedures are presented that support the initiation and controlled expansion of the multipotent adult progenitor cell (MAPC) population within the human bone marrow derived multipotent mesenchymal stromal cell compartment. Culture procedures or conditions and characterization assays that maintain and survey the distinctive primitive MAPC properties are discussed in the context of cell culturing platforms that facilitate controlled expansion of clinical grade human MAPC product to levels required for mid to late stage clinical testing.
ReGenesys BVBA, Heverlee, Belgium.
Bart
B
Vaes
Sara
S
Walbers
Kristel
K
Gijbels
David
D
Craeye
Robert
R
Deans
Jef
J
Pinxteren
Wouter
W
van't Hof
D000328:Adult; D053687:Adult Stem Cells / Q000166:cytology*; D018929:Cell Culture Techniques / Q000379:methods*; D002469:Cell Separation / Q000379:methods; D002478:Cells, Cultured; D015925:Cryopreservation / Q000379:methods; D006801:Humans; D039902:Multipotent Stem Cells / Q000166:cytology*