Population structure, genetic variability, and gene flow of the bean leaf beetle, Cerotoma trifurcata, in the Midwestern United States.
Bean leaf beetle, Cerotoma trifurcata (Forster) (Coleoptera: Chrysomelidae), is a common pest of soybean in the Midwest United States. However, there are currently no reports on the genetic variability of C. trifurcata. This study examined 15-30 individuals from 25 sample locations to estimate genetic variability and gene flow within and among C. trifurcata from across the Mid- west. Amplified fragment length polymorphism generated 175 markers for analyses. Results from analysis of molecular variance (AMOVA) indicated that the majority of genetic variation was from within samples; only a small amount of the total variation was attributed to the variation among the samples. The GST for the entire C. trifurcata population indicated that the majority of genetic variation was found within the samples, further supporting the AMOVA results. The estimated average gene flow among the C. trifurcata samples was 1.83. The Mantel test revealed no indication of correlation between geographical and genetic distance for all the C. trifurcata samples. These findings show that C. trifurcata in the Midwest are genetically heterogeneous and part of a large, interbreeding population.
Department of Entomology, University of Nebraska-Lincoln, Lincoln, NE 68583-0816 btiroese@yahoo.com.
Bamphitlhi
B
Tiroesele
USDA-ARS-KBUSLIRL Screwworm Research Unit, Kerrville, TX 78028 steve.skoda@ars.usda.gov.
Steven R
SR
Skoda
Department of Entomology, University of Nebraska Haskell Agricultural Laboratory, Concord, NE, 68728-2828 thunt2@unl.edu.
Thomas E
TE
Hunt
Department of Agronomy and Horticulture, University of Nebraska-Lincoln, Lincoln, NE 68583-0915 dlee1@unl.edu.
Donald J
DJ
Lee
Coordinación General de Investigación Científica, Centro Universitario de Investigación y Desarrollo Agropecuario, Tecomán Colima 28930, México jmolina18@hotmail.com.
Jaime
J
Molina-Ochoa
Department of Entomology, University of Nebraska-Lincoln, Lincoln, NE 68583-0816 jfoster@unlnotes.unl.edu.
John E
JE
Foster
D063147:Animal Distribution; D000818:Animals; D001517:Coleoptera / Q000235:genetics* / Q000502:physiology*; D051456:Gene Flow; D014644:Genetic Variation; D015146:Midwestern United States
AFLP; genetic distance; geographic distance; soybean pests