Biomarkers identified from serum proteomic analysis for the differential diagnosis of systemic lupus erythematosus.
Systemic lupus erythematosus (SLE) is a chronic autoimmune disease that involves different organs. Its most important feature is the production of specific autoantibodies against nuclear or cytoplasmic antigens. Proteomic analysis of serum, as one of the most readily available body fluids, can be used as a method for clarifying the pathogenesis of SLE. In this study the serum proteome of 13 patients with SLE was evaluated and compared with seven healthy control participants. A specific kit was used to remove high-abundance proteins. After depletion, the protein expression patterns created by two-dimensional gel electrophoresis (2-DE) and MALDI-TOF/TOF-MS were used to identify disease-associated proteins. We found differential expression of 15 protein spots, including seven up-regulated and eight down-regulated proteins in SLE samples, in comparison with healthy participants. These spots were identified by MALDI-TOF/TOF-MS and classified into three groups include keratins, apolipoproteins and albumin, and individual proteins such as transthyretin, haptoglobin and prothrombin. These findings can help to clarify the pathophysiology and mechanism of SLE.
Department of Biochemistry, School of Veterinary Medicine, Shiraz University, Shiraz, Iran Department of Biology, College of Science, University of Sistan and Baluchestan, Zahedan, Iran kazemipour@shirazu.ac.ir.
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Kazemipour
Department of Biology, College of Science, University of Sistan and Baluchestan, Zahedan, Iran.
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Qazizadeh
Gastroenterohepatology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
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Sepehrimanesh
Cellular and Molecular Research Center, Zahedan University of Medical Sciences, Zahedan, Iran.
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Salimi
D000328:Adult; D015415:Biomarkers / Q000097:blood / Q000378:metabolism; D003937:Diagnosis, Differential; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D005260:Female; D006242:Haptoglobins / Q000378:metabolism; D006801:Humans; D008180:Lupus Erythematosus, Systemic / Q000097:blood* / Q000276:immunology / Q000473:pathology; D008297:Male; D011110:Polymorphism, Genetic; D011228:Prealbumin / Q000378:metabolism; D020543:Proteome / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods
D015415:Biomarkers; D006242:Haptoglobins; D011228:Prealbumin; D020543:Proteome
MALDI-TOF/TOF-MS; Systemic lupus erythematosus; haptoglobin; serum proteome; transthyretin