Studies on expression of p14ARF and MDM2 in human thyroid neoplasms.
OBJECTIVE
We sought to evaluate the expression and clinical significance of p14ARF and MDM2 proteins in thyroid neoplasm.


METHODS
Immunohistochemical streptavidin-peroxidase (S-P) method was used to detect the expression of p14ARF and MDM2 proteins in 78 cases of papillary thyroid carcinoma (PTC), 34 cases of papillary thyroid microcarcinoma (PTMC) and 45 cases of thyroid adenoma.


RESULTS
The expression of p14ARF and MDM2 protein differed significantly (P<0.01) among three group. The positivity rate of p14ARF protein in PTC was significantly lower than that in thyroid adenoma (P=0.002) and PTMC (P=0.008). While the positivity rate of MDM2 protein in PTC was significantly higher than that in thyroid adenoma (P=0.000) and PTMC (P=0.009). There was a significant correlation found between the expressions of p14ARF and MDM2 proteins in PTC (P=0.013) and PTMC (P=0.012). Also, a significant correlation was found between p14ARF protein expression and lymph node metastasis in PTC (P=0.011).


CONCLUSIONS
It was concluded that p14ARF and MDM2 proteins might be involved in the induction and development of PTC and PTMC whereas p14ARF also had diagnostic value in determining the biological behavior of PTC.
Department of Pathology the Wuxi No 4 Hospital, Wuxi, China - qixiaowei97@163.com.
X W
XW
Qi
H R
HR
Wu
Y
Y
Yin
S H
SH
Xia
J J
JJ
Feng
Y
Y
Pu
L F
LF
Jin
D000236:Adenoma / Q000737:chemistry* / Q000473:pathology; D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000032:analysis*; D002291:Carcinoma, Papillary / Q000737:chemistry* / Q000473:pathology; D002648:Child; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D011237:Predictive Value of Tests; D051736:Proto-Oncogene Proteins c-mdm2 / Q000032:analysis*; D013964:Thyroid Neoplasms / Q000737:chemistry* / Q000473:pathology; D025803:Tumor Suppressor Protein p14ARF / Q000032:analysis*; D055815:Young Adult
D014408:Biomarkers, Tumor; D025803:Tumor Suppressor Protein p14ARF; C497206:MDM2 protein, human; D051736:Proto-Oncogene Proteins c-mdm2