Evaluation of antibody response of killed and live vaccines against porcine epidemic diarrhea virus in a field study.
BACKGROUND
Porcine epidemic diarrhea virus (PEDV) is an infectious, highly contagious virus, and is an etiological agent of acute entero-pathogenic diarrhea in swine.


OBJECTIVE
Evaluation of the antibody response of two types of PEDV vaccines is to be carried out.


METHODS
Sows were vaccinated with either live or killed commercial PEDV SM98 (GenBank: GU937797.1) vaccines. Four different groups of sows with five sows in each group were used in this study: the unvaccinated negative control group, the killed virus vaccination group with killed virus boosting (K/K), the live virus vaccinated group with live virus boosting (L/L), and the combination group vaccinated with live virus and subsequently boosted with killed vaccine (L/K). Sows were vaccinated intramuscularly twice at four and two weeks prior to farrowing with 2ml/head vaccine dose. Antibody titers in sow and piglet serum one week after farrowing and that in colostrum were compared by enzyme-linked immunosorbent assay (ELISA) and serum neutralization test.


RESULTS
Vaccination with K/K vaccine induced the highest level of IgG and IgA in sow serum, colostrum, and especially in piglet serum, with the lowest levels found in the L/L group. The major neutralizing activity was also found in the K/K group, particularly in colostrum, with piglets bearing higher neutralizing activity compared to sow sera. Among recombinant spike S1, S2, S3, and nucleocapsid N protein of PEDV, S3 protein presented the highest antibody level in the K/K group.


CONCLUSIONS
Killed PEDV SM98 vaccine induced higher antibody levels.


CONCLUSIONS
This study clearly confirms that killed vaccine has induced higher antibody levels and may contribute to the design of future research and vaccine programs.
a Laboratory of Infectious Disease, College of Veterinary Medicine , Chungnam National University , Daejeon , Korea.
S
S
Paudel
J E
JE
Park
H
H
Jang
B H
BH
Hyun
D G
DG
Yang
H J
HJ
Shin
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D018352:Coronavirus Infections / Q000276:immunology / Q000662:veterinary* / Q000821:virology; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005260:Female; D007273:Injections, Intramuscular / Q000662:veterinary; D009500:Neutralization Tests / Q000662:veterinary; D053485:Porcine epidemic diarrhea virus / Q000276:immunology*; D056910:Republic of Korea; D013552:Swine; D013553:Swine Diseases / Q000276:immunology* / Q000821:virology; D014613:Vaccines, Attenuated / Q000276:immunology; D015164:Vaccines, Inactivated / Q000276:immunology; D014765:Viral Vaccines / Q000008:administration & dosage / Q000276:immunology*
D000914:Antibodies, Viral; D014613:Vaccines, Attenuated; D015164:Vaccines, Inactivated; D014765:Viral Vaccines
PEDV; pig; porcine; porcine epidemic diarrhea virus; vaccine