Cell growth behaviors of Clostridium acetobutylicum in a pervaporation membrane bioreactor for butanol fermentation.
Acetone-butanol-ethanol fermentation using Clostridium acetobutylicum was studied in the continuous and closed-circulating fermentation (CCCF) system. The experiment lasting for 192 H was carried out by integrating fermentation with in situ pervaporation. In the entire process, the cell growth profile took place in the following two phases: the logarithmic phase during early 28 H and the linear phase from 130 to 150 H. This was a unique characteristic compared with the curve of traditional fermentation, and the fitting equations of two growth phases were obtained by Origin software according to the kinetic model of cell growth. Besides, the kinetic parameters that include the butanol yield, maximum specific growth rate, average specific formation rate, and volumetric productivity of butanol were measured as 0.19 g g(-1) , 0.345 H(-1) , 0.134 H(-1) and 0.23 g L(-1)  H(-1) , respectively. The C. acetobutylicum in the CCCF system showed good adaptability and fermentation performance, and the prolonged fermentation period and high production were also the main advantages of CCCF technology.
Department of Chemical Engineering, Sichuan University, Chengdu, People's Republic of China.
Peina
P
Yao
Department of Chemical Engineering, Sichuan University, Chengdu, People's Republic of China.
Zeyi
Z
Xiao
Department of Chemical Engineering, Sichuan University, Chengdu, People's Republic of China.|College of Chemistry and Chemical Engineering, Southwest Petroleum University, Chengdu, People's Republic of China.
Chunyan
C
Chen
Department of Chemical Engineering, Sichuan University, Chengdu, People's Republic of China.
Weijia
W
Li
Department of Chemical Engineering, Sichuan University, Chengdu, People's Republic of China.
Qing
Q
Deng
D000096:Acetone / Q000378:metabolism; D019149:Bioreactors / Q000382:microbiology*; D000440:Butanols / Q000378:metabolism*; D046969:Clostridium acetobutylicum / Q000166:cytology / Q000254:growth & development* / Q000378:metabolism; D004867:Equipment Design; D000431:Ethanol / Q000378:metabolism; D005285:Fermentation; D005947:Glucose / Q000378:metabolism; D007700:Kinetics; D008567:Membranes, Artificial
D000440:Butanols; D008567:Membranes, Artificial; D000096:Acetone; D000431:Ethanol; D005947:Glucose
Clostridium acetobutylicum; butanol; cell growth; pervaporation membrane