Optical imaging of fluorescent carbon biomarkers using artificial neural networks.
The principle possibility of extraction of fluorescence of nanoparticles in the presence of background autofluorescence of a biological environment using neural network algorithms is demonstrated. It is shown that the methods used allow detection of carbon nanoparticles fluorescence against the background of the autofluorescence of egg white with a sufficiently low concentration detection threshold (not more than 2 μg/ml for carbon dots 3 μg/ml and for nanodiamonds). It was also shown that the use of the input data compression can further improve the accuracy of solving the inverse problem by 1.5 times.
M. V. Lomonosov Moscow State University, Department of Physics, Leninskie Gory 1/2, Moscow 119991, Russia.
Tatiana A
TA
Dolenko
M. V. Lomonosov Moscow State University, Department of Physics, Leninskie Gory 1/2, Moscow 119991, Russia.
Sergey A
SA
Burikov
M. V. Lomonosov Moscow State University, Department of Physics, Leninskie Gory 1/2, Moscow 119991, Russia.
Alexey M
AM
Vervald
A. M. Prokhorov General Physics Institute, RAS, Vavilova Street 38, Moscow 119991, RussiacNational Research Nuclear University MEPhI, Kashirskoe Avenue 31, Moscow 115409, Russia.
Igor I
II
Vlasov
M. V. Lomonosov Moscow State University, D. V. Skobeltsyn Institute of Nuclear Physics, Leninskie Gory 1/2, Moscow 119991, Russia.
Sergey A
SA
Dolenko
M. V. Lomonosov Moscow State University, Department of Physics, Leninskie Gory 1/2, Moscow 119991, Russia.
Kirill A
KA
Laptinskiy
Abo Akademi University, Laboratory for Physical Chemistry, Porthansgatan 3, 20500 Turku, Finland.
Jessica M
JM
Rosenholm
Adámas Nanotechnologies, Inc., Raleigh, North Carolina 27617, United States.
Olga A
OA
Shenderova
D000818:Animals; D015415:Biomarkers / Q000737:chemistry*; D002244:Carbon / Q000737:chemistry*; D002645:Chickens; D004529:Egg White / Q000737:chemistry; D008956:Models, Chemical; D053758:Nanoparticles / Q000737:chemistry; D016571:Neural Networks, Computer; D061848:Optical Imaging / Q000379:methods*; D013050:Spectrometry, Fluorescence
D015415:Biomarkers; D002244:Carbon