Wogonin prevents rat dorsal root ganglion neurons death via inhibiting tunicamycin-induced ER stress in vitro.
Wogonin is a natural flavonoid isolated from the root of Scutellaria baicalensis Georgi, which has been widely used in various research areas for its anti-oxidant, anti-inflammatory, and anti-cancer activities. It also presents a neuroprotective effect in the brain while encounters stress conditions, but the mechanisms controlling the neuroprotective effect of wogonin are not clear. In this study, we investigated the biomechanism underlying the neuroprotective effect of wogonin on rat dorsal root ganglion (DRG) neurons. Wogonin pre-treatment at 75 μM significantly increased the cell viability of DRG neurons and decreased the number of the propidium iodide-positive DRG neurons before the endoplasmic reticulum (ER) stress is being induced by tunicamycin (TUN) (0.75 μg/mL). In addition, Wogonin also inhibited the release of LDH and up-regulated the level of GSH. Furthermore, wogonin decreased the activation of ER stress-related molecules, including glucose-regulated protein 78 (GRP78), GRP94, C/EBP-homologous protein, active caspase12 and active caspase3, phosphorylation of pancreatic ER stress kinase, and eukaryotic initiation factor 2 alpha (eIF2α). In summary, our results indicated that wogonin could protect DRG neurons against TUN-induced ER stress.
Department of Surgery, Yijishan Hospital, Wannan Medical College, Wuhu, Anhui, People's Republic of China.
Shujuan
S
Xu
Department of Orthopedic Surgery, Yijishan Hospital, Wannan Medical College, Wuhu, Anhui, People's Republic of China.
Xin
X
Zhao
Department of Orthopedic Surgery, Yijishan Hospital, Wannan Medical College, Wuhu, Anhui, People's Republic of China.
Quanlai
Q
Zhao
Department of Orthopedic Surgery, Yijishan Hospital, Wannan Medical College, Wuhu, Anhui, People's Republic of China.
Quan
Q
Zheng
Department of Orthopedic Surgery, Yijishan Hospital, Wannan Medical College, Wuhu, Anhui, People's Republic of China.
Zhen
Z
Fang
Department of Orthopedic Surgery, Yijishan Hospital, Wannan Medical College, Wuhu, Anhui, People's Republic of China.
Xiaoming
X
Yang
Department of Orthopedic Surgery, Yijishan Hospital, Wannan Medical College, Wuhu, Anhui, People's Republic of China.
Hong
H
Wang
Department of Orthopedic Surgery, Yijishan Hospital, Wannan Medical College, Wuhu, Anhui, People's Republic of China.
Ping
P
Liu
Department of Orthopedic Surgery, Yijishan Hospital, Wannan Medical College, Wuhu, Anhui, People's Republic of China. xuhg@medmail.com.cn.
Hongguang
H
Xu
D000818:Animals; D016923:Cell Death / Q000187:drug effects / Q000502:physiology; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D059865:Endoplasmic Reticulum Stress / Q000187:drug effects / Q000502:physiology*; D044950:Flavanones / Q000494:pharmacology*; D005727:Ganglia, Spinal / Q000187:drug effects / Q000378:metabolism*; D018696:Neuroprotective Agents / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014415:Tunicamycin / Q000037:antagonists & inhibitors / Q000633:toxicity*
D004365:Drugs, Chinese Herbal; D044950:Flavanones; D018696:Neuroprotective Agents; D014415:Tunicamycin; C085514:wogonin