Using bio-functionalized magnetic nanoparticles and dynamic nuclear magnetic resonance to characterize the time-dependent spin-spin relaxation time for sensitive bio-detection.
In this work, we report the use of bio-functionalized magnetic nanoparticles (BMNs) and dynamic magnetic resonance (DMR) to characterize the time-dependent spin-spin relaxation time for sensitive bio-detection. The biomarkers are the human C-reactive protein (CRP) while the BMNs are the anti-CRP bound onto dextran-coated Fe3O4 particles labeled as Fe3O4-antiCRP. It was found the time-dependent spin-spin relaxation time, T2, of protons decreases as time evolves. Additionally, the ΔT2 of of protons in BMNs increases as the concentration of CRP increases. We attribute these to the formation of the magnetic clusters that deteriorate the field homogeneity of nearby protons. A sensitivity better than 0.1 μg/mL for assaying CRP is achieved, which is much higher than that required by the clinical criteria (0.5 mg/dL). The present MR-detection platform shows promise for further use in detecting tumors, viruses, and proteins.
Institute of Electro-Optical Science and Technology, National Taiwan Normal University, Taipei 116, Taiwan. shliao@ntnu.edu.tw.
Shu-Hsien
SH
Liao
Department of Electro-Optical Engineering, Kun Shan University, Tainan 710, Taiwan. chwu@phys.nchu.edu.tw.
Kuen-Lin
KL
Chen
Institute of Electro-Optical Science and Technology, National Taiwan Normal University, Taipei 116, Taiwan. u20522@hotmail.com.
Chun-Min
CM
Wang
Institute of Electro-Optical Science and Technology, National Taiwan Normal University, Taipei 116, Taiwan. jjchieh@ntnu.edu.tw.
Jen-Jie
JJ
Chieh
Institute of Electro-Optical Science and Technology, National Taiwan Normal University, Taipei 116, Taiwan. phyfv001@ntnu.edu.tw.
Herng-Er
HE
Horng
Graduate Institute of Applied Physics and Department of Physics, National Taiwan University, Taipei 106, Taiwan. liminwang@ntu.edu.tw.
Li-Min
LM
Wang
Department of Physics, National Chung Hsing University, Taichung 402, Taiwan. chwu@phys.nchu.edu.tw.
C H
CH
Wu
Department of Electro-Optical Engineering, Kun Shan University, Tainan 710, Taiwan. hcyang@phys.ntu.edu.tw.
Hong-Chang
HC
Yang
D015374:Biosensing Techniques / Q000295:instrumentation*; D002097:C-Reactive Protein / Q000032:analysis* / Q000276:immunology; D020099:Coated Materials, Biocompatible / Q000138:chemical synthesis; D003911:Dextrans / Q000737:chemistry*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006801:Humans; D018189:Immunomagnetic Separation / Q000295:instrumentation*; D009682:Magnetic Resonance Spectroscopy / Q000295:instrumentation*; D058185:Magnetite Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013113:Spin Labels
D020099:Coated Materials, Biocompatible; D003911:Dextrans; D058185:Magnetite Nanoparticles; D013113:Spin Labels; D002097:C-Reactive Protein