Carbon nanoparticle-based ratiometric fluorescent sensor for detecting mercury ions in aqueous media and living cells.
A novel nanohybrid ratiometric fluorescence sensor is developed for selective detection of mercuric ions (Hg(2+)), and the application has been successfully demonstrated in HEPES buffer solution, lake water, and living cells. The sensor comprises water-soluble fluorescent carbon nanoparticles (CNPs) and Rhodamine B (RhB) and exhibits their corresponding dual emissions peaked at 437 and 575 nm, respectively, under a single excitation wavelength (350 nm). The photoluminescence of the CNPs in the nanohybrid system can be completely quenched by Hg(2+) through effective electron or energy transfer process due to synergetic strong electrostatic interaction and metal-ligand coordination between the surface functional group of CNPs and Hg(2+), while that of the RhB remains constant. This results in an obviously distinguishable fluorescence color variation (from violet to orange) of the nanohybrid solution. This novel sensor can effectively identify Hg(2+) from other metal ions with relatively low background interference even in a complex system such as lake water. The detection limit of this method is as low as 42 nM. Furthermore, the sensing technique is applicable to detect Hg(2+) in living cells.
Center of Super-Diamond and Advanced Films (COSDAF) and Department of Physics and Materials Science, City University of Hong Kong , Hong Kong SAR, People's Republic of China.
Minhuan
M
Lan
Jinfeng
J
Zhang
Ying-San
YS
Chui
Pengfei
P
Wang
Xianfeng
X
Chen
Chun-Sing
CS
Lee
Hoi-Lun
HL
Kwong
Wenjun
W
Zhang
D002244:Carbon / Q000737:chemistry; D007477:Ions / Q000737:chemistry / Q000302:isolation & purification*; D057230:Limit of Detection; D008628:Mercury / Q000737:chemistry / Q000302:isolation & purification*; D053758:Nanoparticles / Q000737:chemistry; D045663:Quantum Dots; D012235:Rhodamines / Q000737:chemistry; D014867:Water / Q000737:chemistry*; D014874:Water Pollutants, Chemical / Q000737:chemistry / Q000302:isolation & purification*
D007477:Ions; D012235:Rhodamines; D014874:Water Pollutants, Chemical; D014867:Water; D002244:Carbon; D008628:Mercury; C029773:rhodamine B
bioimaging; carbon nanoparticles; mercury ions; ratiometric fluorescence sensor