Analysis of biomolecular dynamics by FRAP and computer simulation.
We present a Monte Carlo simulation environment for modelling complex biological molecular interaction networks and for the design, validation, and quantitative analysis of FRAP assays to study these. The program is straightforward in its implementation and can be instructed through an intuitive script language. The simulation tool fits very well in a systems biology research setting that aims to maintain an interactive cycle of experiment-driven modelling and model-driven experimentation: the model and the experiment are in the same simulation. The full program can be obtained by request to the authors.
Department of Pathology, Josephine Nefkens Institute, Erasmus Optical Imaging Centre, Erasmus MC, Postbus 2040, Rotterdam, 3000 CA, The Netherlands.
Bart
B
Geverts
Martin E
ME
van Royen
Adriaan B
AB
Houtsmuller
D000465:Algorithms; D004058:Diffusion; D036681:Fluorescence Recovery After Photobleaching / Q000379:methods*; D008954:Models, Biological; D056004:Molecular Dynamics Simulation; D009010:Monte Carlo Method; D011485:Protein Binding; D049490:Systems Biology / Q000379:methods*; D014584:User-Computer Interface