Diethylcarbamazine reduces chronic inflammation and fibrosis in carbon tetrachloride- (CCl₄-) induced liver injury in mice.
This study investigated the anti-inflammatory effects of DEC on the CCl4-induced hepatotoxicity in C57BL/6 mice. Chronic inflammation was induced by i.p. administration of CCl4 0.5 μL/g of body weight through two injections a week for 6 weeks. DEC (50 mg/kg) was administered by gavage for 12 days before finishing the CCl4 induction. Histological analyses of the DEC-treated group exhibited reduced inflammatory process and prevented liver necrosis and fibrosis. Immunohistochemical and immunofluorescence analyses of the DEC-treated group showed reduced COX-2, IL1β, MDA, TGF-β, and αSMA immunopositivity, besides exhibiting decreased IL1β, COX-2, NFκB, IFNγ, and TGFβ expressions in the western blot analysis. The DEC group enhanced significantly the IL-10 expression. The reduction of hepatic injury in the DEC-treated group was confirmed by the COX-2 and iNOS mRNA expression levels. Based on the results of the present study, DEC can be used as a potential anti-inflammatory drug for chronic hepatic inflammation.
Laboratory of Ultrastructure, Aggeu Magalhães Research Center (CPqAM), Avenida Professor Moraes Rego, s/n, Cidade Universitária, 50740-465 Recife, PE, Brazil ; Department of Entomology, Aggeu Magalhães Research Center (CPqAM), Avenida Professor Moraes Rego, s/n, Cidade Universitária, 50740-465 Recife, PE, Brazil.
Sura Wanessa Santos
SW
Rocha
Laboratory of Ultrastructure, Aggeu Magalhães Research Center (CPqAM), Avenida Professor Moraes Rego, s/n, Cidade Universitária, 50740-465 Recife, PE, Brazil.
Maria Eduarda Rocha
ME
de França
Laboratory of Ultrastructure, Aggeu Magalhães Research Center (CPqAM), Avenida Professor Moraes Rego, s/n, Cidade Universitária, 50740-465 Recife, PE, Brazil.
Gabriel Barros
GB
Rodrigues
Laboratory of Ultrastructure, Aggeu Magalhães Research Center (CPqAM), Avenida Professor Moraes Rego, s/n, Cidade Universitária, 50740-465 Recife, PE, Brazil.
Karla Patrícia Sousa
KP
Barbosa
Laboratory of Ultrastructure, Aggeu Magalhães Research Center (CPqAM), Avenida Professor Moraes Rego, s/n, Cidade Universitária, 50740-465 Recife, PE, Brazil.
Ana Karolina Santana
AK
Nunes
Laboratory of Virology and Experimental Therapy, Aggeu Magalhães Research Center (CPqAM), Avenida Professor Moraes Rego, s/n, Cidade Universitária, 50740-465 Recife, PE, Brazil.
André Filipe
AF
Pastor
Laboratory of Microscopy and Microanalisis, Northeastern Center for Strategic Technologies (CETENE), Avenida Professor Luiz Freire, Cidade Universitária, 50740-540 Recife, PE, Brazil.
Anne Gabrielle Vasconcelos
AG
Oliveira
Laboratory of Ultrastructure, Aggeu Magalhães Research Center (CPqAM), Avenida Professor Moraes Rego, s/n, Cidade Universitária, 50740-465 Recife, PE, Brazil.
Wilma Helena
WH
Oliveira
Laboratory of Ultrastructure, Aggeu Magalhães Research Center (CPqAM), Avenida Professor Moraes Rego, s/n, Cidade Universitária, 50740-465 Recife, PE, Brazil.
Rayana Leal Almeida
RL
Luna
Laboratory of Ultrastructure, Aggeu Magalhães Research Center (CPqAM), Avenida Professor Moraes Rego, s/n, Cidade Universitária, 50740-465 Recife, PE, Brazil.
Christina Alves
CA
Peixoto
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D002251:Carbon Tetrachloride / Q000633:toxicity; D056486:Chemical and Drug Induced Liver Injury / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D003094:Collagen / Q000378:metabolism; D051546:Cyclooxygenase 2 / Q000235:genetics / Q000378:metabolism; D016207:Cytokines / Q000378:metabolism; D004049:Diethylcarbamazine / Q000494:pharmacology*; D004195:Disease Models, Animal; D015870:Gene Expression / Q000187:drug effects; D007249:Inflammation / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology; D008103:Liver Cirrhosis / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009569:Nitric Oxide / Q000378:metabolism; D052247:Nitric Oxide Synthase Type II / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
D000893:Anti-Inflammatory Agents; D016207:Cytokines; D012333:RNA, Messenger; D009569:Nitric Oxide; D003094:Collagen; D002251:Carbon Tetrachloride; D052247:Nitric Oxide Synthase Type II; C496317:Nos2 protein, mouse; C077623:Ptgs2 protein, mouse; D051546:Cyclooxygenase 2; D004049:Diethylcarbamazine