Rhizobium sophorae sp. nov. and Rhizobium sophoriradicis sp. nov., nitrogen-fixing rhizobial symbionts of the medicinal legume Sophora flavescens.
Five bacterial strains representing 45 isolates originated from root nodules of the medicinal legume Sophora flavescens were defined as two novel groups in the genus Rhizobium based on their phylogenetic relationships estimated from 16S rRNA genes and the housekeeping genes recA, glnII and atpD. These groups were distantly related to Rhizobium leguminosarum USDA 2370(T) (95.6 % similarity for group I) and Rhizobium phaseoli ATCC 14482(T) (93.4 % similarity for group II) in multilocus sequence analysis. In DNA-DNA hybridization experiments, the reference strains CCBAU 03386(T) (group I) and CCBAU 03470(T) (group II) showed levels of relatedness of 17.9-57.8 and 11.0-42.9 %, respectively, with the type strains of related species. Both strains CCBAU 03386(T) and CCBAU 03470(T) contained ubiquinone 10 (Q-10) as the major respiratory quinone and possessed 16 : 0, 18 : 0, 19 : 0 cyclo ω8c, summed feature 8 and summed feature 2 as major fatty acids, but did not contain 20 : 3 ω6,8,12c. Phenotypic features distinguishing both groups from all closely related species of the genus Rhizobium were found. Therefore, two novel species, Rhizobium sophorae sp. nov. for group I (type strain CCBAU 03386(T) = E5(T) = LMG 27901(T) = HAMBI 3615(T)) and Rhizobium sophoriradicis sp. nov. for group II (type strain CCBAU 03470(T) = C-5-1(T) = LMG 27898(T) = HAMBI 3510(T)), are proposed. Both groups were able to nodulate Phaseolus vulgaris and their hosts of origin (Sophora flavescens) effectively and their nodulation gene nodC was phylogenetically located in the symbiovar phaseoli.
MOA Key Laboratory of Soil Microbiology, Beijing 100193, PR China.|College of Biological Sciences and Rhizobia Research Center, China Agricultural University, Beijing 100193, PR China.|State Key Laboratory of Agrobiotechnology, Beijing 100193, PR China.
Yin Shan
YS
Jiao
MOA Key Laboratory of Soil Microbiology, Beijing 100193, PR China.|College of Biological Sciences and Rhizobia Research Center, China Agricultural University, Beijing 100193, PR China.|State Key Laboratory of Agrobiotechnology, Beijing 100193, PR China.
Hui
H
Yan
MOA Key Laboratory of Soil Microbiology, Beijing 100193, PR China.|College of Biological Sciences and Rhizobia Research Center, China Agricultural University, Beijing 100193, PR China.|State Key Laboratory of Agrobiotechnology, Beijing 100193, PR China.
Zhao Jun
ZJ
Ji
MOA Key Laboratory of Soil Microbiology, Beijing 100193, PR China.|College of Biological Sciences and Rhizobia Research Center, China Agricultural University, Beijing 100193, PR China.|State Key Laboratory of Agrobiotechnology, Beijing 100193, PR China.
Yuan Hui
YH
Liu
MOA Key Laboratory of Soil Microbiology, Beijing 100193, PR China.|College of Biological Sciences and Rhizobia Research Center, China Agricultural University, Beijing 100193, PR China.|State Key Laboratory of Agrobiotechnology, Beijing 100193, PR China.
Xin Hua
XH
Sui
MOA Key Laboratory of Soil Microbiology, Beijing 100193, PR China.|College of Biological Sciences and Rhizobia Research Center, China Agricultural University, Beijing 100193, PR China.|Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, México D. F. 11340, México.|State Key Laboratory of Agrobiotechnology, Beijing 100193, PR China.
En Tao
ET
Wang
Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193, PR China.
Bao Lin
BL
Guo
MOA Key Laboratory of Soil Microbiology, Beijing 100193, PR China.|College of Biological Sciences and Rhizobia Research Center, China Agricultural University, Beijing 100193, PR China.|State Key Laboratory of Agrobiotechnology, Beijing 100193, PR China.
Wen Xin
WX
Chen
MOA Key Laboratory of Soil Microbiology, Beijing 100193, PR China.|College of Biological Sciences and Rhizobia Research Center, China Agricultural University, Beijing 100193, PR China.|State Key Laboratory of Agrobiotechnology, Beijing 100193, PR China.
Wen Feng
WF
Chen
D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D009586:Nitrogen Fixation; D009693:Nucleic Acid Hybridization; D027805:Phaseolus; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012231:Rhizobium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D053204:Root Nodules, Plant / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D029909:Sophora / Q000382:microbiology*; D013559:Symbiosis; D014451:Ubiquinone / Q000737:chemistry
D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D014451:Ubiquinone; C025204:Ubiquinone Q2