R-eriodictyol and S-eriodictyol exhibited comparable effect against H2O2-induced oxidative stress in EA.hy926 cells.
Eriodictyol is a flavanone well-known for its antioxidative activity. Due to a chiral carbon atom in position C-2, eriodictyol always exist in racemic form. In order to study the a Chiral Amylose-C column as chiral stationary phase. Online coupling HPLC-circular dichroism (CD) method was used for the determination of elution order and the absolute configurations of the two eluates. The protective effects of racemic and enantiomeric eriodictyol against H2O2-induced cytotoxicity with EA.hy926 cells were tested. The results showed that the two enantiomers of eriodictyol and the corresponding racemate were equipotent, suggesting that the configuration of the C-2 chiral center does not influence the cytoprotective activity against H2O2-induced oxidative stress in EA.hy926 cells.
Key Laboratory of Chemical Biology (Ministry of Education), School of Pharmaceutical Sciences, Shandong University.
Haizhen
H
Li
Chao
C
Li
Tao
T
Shen
Lijuan
L
Zhao
Dongmei
D
Ren
D017209:Apoptosis / Q000187:drug effects; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D044950:Flavanones / Q000494:pharmacology*; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D016877:Oxidants / Q000494:pharmacology*; D018384:Oxidative Stress / Q000187:drug effects*; D017382:Reactive Oxygen Species / Q000378:metabolism; D013237:Stereoisomerism
D044950:Flavanones; D016877:Oxidants; D017382:Reactive Oxygen Species; D006861:Hydrogen Peroxide; C007619:eriodictyol