Protein modeling and molecular dynamics simulation of the two novel surfactant proteins SP-G and SP-H.
Surfactant proteins are well known from the human lung where they are responsible for the stability and flexibility of the pulmonary surfactant system. They are able to influence the surface tension of the gas-liquid interface specifically by directly interacting with single lipids. This work describes the generation of reliable protein structure models to support the experimental characterization of two novel putative surfactant proteins called SP-G and SP-H. The obtained protein models were complemented by predicted posttranslational modifications and placed in a lipid model system mimicking the pulmonary surface. Molecular dynamics simulations of these protein-lipid systems showed the stability of the protein models and the formation of interactions between protein surface and lipid head groups on an atomic scale. Thereby, interaction interface and strength seem to be dependent on orientation and posttranslational modification of the protein. The here presented modeling was fundamental for experimental localization studies and the simulations showed that SP-G and SP-H are theoretically able to interact with lipid systems and thus are members of the surfactant protein family.
Institute of Anatomy, Department II, Friedrich Alexander University Erlangen-Nuremberg, Erlangen, Germany, felix.rausch@anatomie2.med.uni-erlangen.de.
Felix
F
Rausch
Martin
M
Schicht
Lars
L
Bräuer
Friedrich
F
Paulsen
Wolfgang
W
Brandt
D015060:1,2-Dipalmitoylphosphatidylcholine / Q000737:chemistry; D056004:Molecular Dynamics Simulation; D011487:Protein Conformation; D011499:Protein Processing, Post-Translational; D055550:Protein Stability; D037701:Pulmonary Surfactant-Associated Protein B / Q000737:chemistry*; D037661:Pulmonary Surfactant-Associated Proteins / Q000737:chemistry* / Q000378:metabolism; D013329:Structure-Activity Relationship; D013499:Surface Properties
D037701:Pulmonary Surfactant-Associated Protein B; D037661:Pulmonary Surfactant-Associated Proteins; D015060:1,2-Dipalmitoylphosphatidylcholine