Contribution of donor and host mesenchyme to the transplanted tooth germs.
Autologous tooth germ transplantation of immature teeth is an alternative method of tooth replacement that could be used instead of dental implants in younger patients. However, it is paramount that the dental pulp remain vital and that root formation continue in the transplanted location. The goal of this study is to characterize the healing of allogenic tooth grafts in an animal model using GFP-labeled donor or host postnatal mice. In addition, the putative stem cells were labeled before transplantation with a pulse-chase paradigm. Transplanted molars formed cusps and roots and erupted into occlusion by 2 wk postoperatively. Host label-retaining cells (LRCs) were maintained in the center of pulp tissue associating with blood vessels. Dual labeling showed that a proportion of LRCs were incorporated into the odontoblast layer. Host cells, including putative dendritic cells and the endothelium, also immigrated into the pulp tissue but did not contribute to the odontoblast layer. Therefore, LRCs or putative mesenchymal stem cells are retained in the transplanted pulps. Hertwig's epithelial root sheath remains vital, and epithelial LRCs are present in the donor cervical loops. Thus, the dynamic donor-host interaction occurred in the developing transplant, suggesting that these changes affect the characteristics of the dental pulp.
Division of Anatomy and Cell Biology of the Hard Tissue, Department of Tissue Regeneration and Reconstruction, Niigata University Graduate School of Medical and Dental Sciences, Niigata, Japan.
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Nakaki
Division of Anatomy and Cell Biology of the Hard Tissue, Department of Tissue Regeneration and Reconstruction, Niigata University Graduate School of Medical and Dental Sciences, Niigata, Japan.
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Saito
Division of Anatomy and Cell Biology of the Hard Tissue, Department of Tissue Regeneration and Reconstruction, Niigata University Graduate School of Medical and Dental Sciences, Niigata, Japan.
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Ida-Yonemochi
Division of Anatomy and Cell Biology of the Hard Tissue, Department of Tissue Regeneration and Reconstruction, Niigata University Graduate School of Medical and Dental Sciences, Niigata, Japan.
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Nakagawa
Division of Anatomy and Cell Biology of the Hard Tissue, Department of Tissue Regeneration and Reconstruction, Niigata University Graduate School of Medical and Dental Sciences, Niigata, Japan.
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Kenmotsu
Division of Anatomy and Cell Biology of the Hard Tissue, Department of Tissue Regeneration and Reconstruction, Niigata University Graduate School of Medical and Dental Sciences, Niigata, Japan histoman@dent.niigata-u.ac.jp.
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Ohshima
D064591:Allografts / Q000166:cytology / Q000637:transplantation*; D000818:Animals; D017209:Apoptosis / Q000502:physiology; D002465:Cell Movement / Q000502:physiology; D049109:Cell Proliferation / Q000502:physiology; D003713:Dendritic Cells / Q000166:cytology; D003771:Dental Papilla / Q000166:cytology; D003782:Dental Pulp / Q000098:blood supply / Q000166:cytology; D003810:Dentinogenesis / Q000502:physiology; D042783:Endothelial Cells / Q000166:cytology; D004730:Endothelium, Vascular / Q000166:cytology; D004847:Epithelial Cells / Q000166:cytology; D049452:Green Fluorescent Proteins; D059630:Mesenchymal Stem Cells / Q000502:physiology; D008648:Mesoderm / Q000166:cytology*; D051379:Mice; D008810:Mice, Inbred C57BL; D008815:Mice, Inbred Strains; D008822:Mice, Transgenic; D023421:Models, Animal; D008963:Molar / Q000166:cytology / Q000502:physiology / Q000637:transplantation*; D009804:Odontoblasts / Q000166:cytology; D009805:Odontogenesis / Q000502:physiology; D019228:Tooth Crown / Q000502:physiology; D014078:Tooth Eruption / Q000502:physiology; D014083:Tooth Germ / Q000166:cytology / Q000502:physiology / Q000637:transplantation*; D014092:Tooth Root / Q000502:physiology
D049452:Green Fluorescent Proteins
bromodeoxyuridine; cell differentiation; cell proliferation; dental pulp; mice (inbred B6); odontoblasts