Allele frequencies for 13 STRs loci in a Western Anatolia population and their forensic evaluation.
BACKGROUND
Numerous studies demonstrated that STRs have become powerful tools in forensic case work.


OBJECTIVE
To profile DNA samples from 104 Turkish males for 13 autosomal, STR markers intended for human identification purposes and to estimate the allele frequency distribution in forensic cases in a Turkish population.


METHODS
Thirteen autosomal STR loci, namely D3S1358, D2S1338, D16S539, D8S1179, D21S11, D18S51, TH01, D13S317, D7S820, CSF1PO, TPOX, D5S818 and FGA, were analysed in a sample of 104 healthy and unrelated Turkish individuals who have been living in the city of İzmir. All loci were amplified by using AmpFlSTR Identifier Kit. Genetic analysis was carried out on an ABI PRISM 310 Genetic Analyser.


RESULTS
For each locus, 6-15 alleles were found with frequencies ranging from 0.005-0.514 and heterozygosities ranging from 0.686-0.868. The PIC value was highly significant (0.999).


CONCLUSIONS
The 13 STR loci in the AmpFlSTR Identifier Kit are suitable for forensic identification and paternity tests due to high heterozygosity. The observed PD value is sufficiently high for human identification purposes. In conclusion, the 13 STR loci seem to be useful markers for personal identification and forensic case work in the Turkish population. The results also demonstrate the importance of region-specific studies.
a Department of Forensic Medicine, Faculty of Medicine , University of Gaziantep , Gaziantep , Turkey .
Aysun
A
Baransel Isir
b Department of Medical Biology, Faculty of Science , University of Ege , İzmir , Turkey .
Abdulmuttalip
A
Ozkorkmaz
c Department of Medical Biology, Faculty of Medicine , University of Gaziantep , Gaziantep , Turkey , and.|d Department of Medical Biology, Faculty of Medicine , University of Istanbul , Istanbul , Turkey.
Sacide
S
Pehlivan
D000483:Alleles; D005260:Female; D053803:Forensic Genetics / Q000379:methods*; D005787:Gene Frequency / Q000235:genetics*; D005819:Genetic Markers / Q000235:genetics*; D005820:Genetic Testing; D014644:Genetic Variation; D005828:Genetics, Population; D006801:Humans; D008297:Male; D018895:Microsatellite Repeats / Q000235:genetics*; D016133:Polymerase Chain Reaction; D014421:Turkey
D005819:Genetic Markers
Forensic science; population genetics; short tandem repeats