Three-dimensional optical coherence micro-elastography of skeletal muscle tissue.
In many muscle pathologies, impairment of skeletal muscle function is closely linked to changes in the mechanical properties of the muscle constituents. Optical coherence micro-elastography (OCME) uses optical coherence tomography (OCT) imaging of tissue under a quasi-static, compressive mechanical load to map variations in tissue mechanical properties on the micro-scale. We present the first study of OCME on skeletal muscle tissue. We show that this technique can resolve features of muscle tissue including fibers, fascicles and tendon, and can also detect necrotic lesions in skeletal muscle from the mdx mouse model of Duchenne muscular dystrophy. In many instances, OCME provides better or additional contrast complementary to that provided by OCT. These results suggest that OCME could provide new understanding and opportunity for assessment of skeletal muscle pathologies.
Optical + Biomedical Engineering Laboratory, School of Electrical, Electronic & Computer Engineering, The University of Western Australia, Crawley, Australia.
Lixin
L
Chin
Optical + Biomedical Engineering Laboratory, School of Electrical, Electronic & Computer Engineering, The University of Western Australia, Crawley, Australia.
Brendan F
BF
Kennedy
Optical + Biomedical Engineering Laboratory, School of Electrical, Electronic & Computer Engineering, The University of Western Australia, Crawley, Australia.
Kelsey M
KM
Kennedy
Optical + Biomedical Engineering Laboratory, School of Electrical, Electronic & Computer Engineering, The University of Western Australia, Crawley, Australia.
Philip
P
Wijesinghe
School of Anatomy, Physiology & Human Biology, The University of Western Australia, Crawley, Australia.
Gavin J
GJ
Pinniger
School of Anatomy, Physiology & Human Biology, The University of Western Australia, Crawley, Australia ; School of Biomedical, Biomolecular & Chemical Science, The University of Western Australia, Crawley, Australia.
Jessica R
JR
Terrill
Optical + Biomedical Engineering Laboratory, School of Electrical, Electronic & Computer Engineering, The University of Western Australia, Crawley, Australia.
Robert A
RA
McLaughlin
Optical + Biomedical Engineering Laboratory, School of Electrical, Electronic & Computer Engineering, The University of Western Australia, Crawley, Australia ; Centre for Microscopy, Characterisation & Analysis, The University of Western Australia, Crawley, Australia.
David D
DD
Sampson
(110.1650) Coherence imaging; (170.3880) Medical and biological imaging; (170.4500) Optical coherence tomography; (170.6935) Tissue characterization; (280.1415) Biological sensing and sensors