A medium throughput device to study the effects of combinations of surface strains and fluid-flow shear stresses on cells.
We report a medium throughput device to study the effects of combinations of two mechanical stimuli - surface strains and fluid flow shear stresses, on cells. The first generation prototype can screen combinations of five strain and five shear stress levels. Computational modeling and empirical measurements were used to determine the generated strains and flows. Uniform equibiaxial strains up to 20% and shear stresses up to 0.3 Pa can be generated. Compatibility of the device with cell culture and end point fixation, staining and imaging is shown using C2C12 mouse myoblast cells.
Department of Biomechanical Engineering, MIRA Institute for Biomedical Technology and Technical Medicine, University of Twente, Enschede, The Netherlands. j.rouwkema@utwente.nl.
R
R
Sinha
S
S
Le Gac
N
N
Verdonschot
A
A
van den Berg
B
B
Koopman
J
J
Rouwkema
D000818:Animals; D018929:Cell Culture Techniques / Q000295:instrumentation* / Q000379:methods; D002460:Cell Line; D003198:Computer Simulation; D004867:Equipment Design; D051379:Mice; D011312:Pressure; D033081:Shear Strength