Sealing of vessels larger than 7 millimeters using Enseal in porcine aorta.
BACKGROUND
The Enseal (Ethicon Endo-Surgery, Blue Ash, Ohio) tissue-sealing device has proven efficacy for ligation of vessels<7 mm in diameter, even with significant supraphysiologic bursting pressures. We aimed to evaluate the safety of Enseal in porcine vessels>7 mm.


METHODS
The lumbar aortas of pigs that were euthanized for unrelated procedures were harvested. A 5- to 6-cm segment of aorta was sealed using the Enseal device. The opposite end was attached to a pressure-testing device to measure pressures at leak or bursting. The bivariate Pearson correlation was used to determine the relationship between diameter and bursting pressure. One-way analysis of variance was used to determine differences between the groups of vessels on the basis of their diameter.


RESULTS
Ninety samples of 5-cm aorta segments were used to assess bursting pressure. The median diameter was 14 mm (range, 7-18) and bursting pressure was 85 mm Hg (range, 24-650). The Pearson test showed a negative correlation between vessel diameter and bursting pressure (P=.25). One-way analysis of variance did not show any significant difference between vessel diameters grouped by size (P=.517), and neither did the Scheffe post hoc test when comparing diameter with bursting pressure; 31% of specimens failed to seal.


CONCLUSIONS
Bursting pressures are low and inconsistent after tissue sealing with the Enseal device in porcine vessels>7 mm. These vessels also demonstrated a higher rate of failure to seal. The histologic results of the aorta segments (ie, a low collagen-elastin ratio) may be the cause of the low bursting pressures.
Department of Colorectal Surgery, University of Illinois at Chicago, Chicago, IL, USA.
Shahida
S
Bibi
Department of Colorectal Surgery, University of Illinois at Chicago, Chicago, IL, USA.
Saeed
S
Alblawi
Department of Colorectal Surgery, University of Illinois at Chicago, Chicago, IL, USA.
Vamsi
V
Velchuru
Department of Colorectal Surgery, University of Illinois at Chicago, Chicago, IL, USA.
Gopichand
G
Thripuraneni
Division of Colorectal Surgery, Advocate Lutheran General Hospital, Park Ridge, IL, USA.
Francisco
F
Quinteros
Department of Colorectal Surgery, University of Illinois at Chicago, Chicago, IL, USA.
Jasna
J
Coralic
Division of Colorectal Surgery, Advocate Lutheran General Hospital, Park Ridge, IL, USA.
Slawomir
S
Marecik
Division of Colorectal Surgery, Advocate Lutheran General Hospital, Park Ridge, IL, USA.
John
J
Park
Department of Colorectal Surgery, University of Illinois at Chicago, Chicago, IL, USA.
Leela M
LM
Prasad
D000818:Animals; D001012:Aorta, Abdominal / Q000601:surgery*; D004195:Disease Models, Animal; D057510:Endovascular Procedures / Q000295:instrumentation*; D005260:Female; D008026:Ligation; D011312:Pressure; D013536:Suture Techniques / Q000295:instrumentation*; D013537:Sutures; D013552:Swine
Bursting pressure; Collagen; Elastin; Enseal; Porcine aorta; Vessels