New tyrosinase inhibitors from Paecilomyces gunnii.
Through screening 50 strains of entomopathogenic fungi and rescreening of 7 strains of Paecilomyces gunnii, a methanol extract of liquid-cultivated mycelia of P. gunnii was found to have the strongest tyrosinase inhibitory activity. Preparative high-speed counter-current chromatography (HSCCC) guided by high-performance liquid chromatography (HPLC)-electrospray ionization (ESI)-high-resolution mass spectrometry (HRMS) was employed for the isolation and purification of the active components, and three new compounds with half inhibition concentration (IC50) of 0.11, 0.17, and 0.14 mM against diphenolase were obtained from the extract, respectively. Their chemical structures were identified by HRMS, one- and two-dimensional nuclear magnetic resonance (2D NMR) spectroscopy as paecilomycones A, B, and C. Structure and activity studies showed that the tyrosinase inhibition activities are positively related to the number of hydroxyl groups on the paecilomycones.
Anhui Agricultural University , Hefei, Anhui 230036, People's Republic of China.
Ruili
R
Lu
Xiaoxiao
X
Liu
Sha
S
Gao
Wancun
W
Zhang
Fan
F
Peng
Fenglin
F
Hu
Bo
B
Huang
Longyun
L
Chen
Guanhu
G
Bao
Chunru
C
Li
Zengzhi
Z
Li
D001685:Biological Factors / Q000737:chemistry* / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid; D003377:Countercurrent Distribution; D004791:Enzyme Inhibitors / Q000737:chemistry* / Q000302:isolation & purification; D015394:Molecular Structure; D014442:Monophenol Monooxygenase / Q000037:antagonists & inhibitors*; D010143:Paecilomyces / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization
D001685:Biological Factors; D004791:Enzyme Inhibitors; D014442:Monophenol Monooxygenase
Paecilomyces gunnii; phenalenones; tyrosinase inhibition