In vitro selection of a single-stranded DNA molecular recognition element specific for bromacil.
Bromacil is a widely used herbicide that is known to contaminate environmental systems. Due to the hazards it presents and inefficient detection methods, it is necessary to create a rapid and efficient sensing device. Towards this end, we have utilized a stringent in vitro selection method to identify single-stranded DNA molecular recognition elements (MRE) specific for bromacil. We have identified one MRE with high affinity (K d = 9.6 nM) and specificity for bromacil compared to negative targets of selection and other pesticides. The selected ssDNA MRE will be useful as the sensing element in a field-deployable bromacil detection device.
Department of Basic Pharmaceutical Sciences, West Virginia University, 1 Medical Center Drive, P.O. Box 9530, Morgantown, WV 26506, USA ; Memorial Sloan Kettering Cancer Center, Molecular Pharmacology & Chemistry Program, 1275 York Avenue, New York, NY 10065, USA.
Ryan M
RM
Williams
Department of Basic Pharmaceutical Sciences, West Virginia University, 1 Medical Center Drive, P.O. Box 9530, Morgantown, WV 26506, USA.
Amanda R
AR
Kulick
Department of Chemistry, West Virginia University, 217 Clark Hall, Morgantown, WV 26506, USA.
Srilakshmi
S
Yedlapalli
Department of Basic Pharmaceutical Sciences, West Virginia University, 1 Medical Center Drive, P.O. Box 9530, Morgantown, WV 26506, USA.
Louisa
L
Battistella
Department of Basic Pharmaceutical Sciences, West Virginia University, 1 Medical Center Drive, P.O. Box 9530, Morgantown, WV 26506, USA.
Cyrus J
CJ
Hajiran
Department of Basic Pharmaceutical Sciences, West Virginia University, 1 Medical Center Drive, P.O. Box 9530, Morgantown, WV 26506, USA.
Letha J
LJ
Sooter