Cellulolytic enzymes production by utilizing agricultural wastes under solid state fermentation and its application for biohydrogen production.
Phanerochaete chrysosporium was evaluated for cellulase and hemicellulase production using various agricultural wastes under solid state fermentation. Optimization of various environmental factors, type of substrate, and medium composition was systematically investigated to maximize the production of enzyme complex. Using grass powder as a carbon substrate, maximum activities of endoglucanase (188.66 U/gds), exoglucanase (24.22 U/gds), cellobiase (244.60 U/gds), filter paperase (FPU) (30.22 U/gds), glucoamylase (505.0 U/gds), and xylanase (427.0 U/gds) were produced under optimized conditions. The produced crude enzyme complex was employed for hydrolysis of untreated and mild acid pretreated rice husk. The maximum amount of reducing sugar released from enzyme treated rice husk was 485 mg/g of the substrate. Finally, the hydrolysates of rice husk were used for hydrogen production by Clostridium beijerinckii. The maximum cumulative H2 production and H2 yield were 237.97 mL and 2.93 mmoL H2/g of reducing sugar, (or 2.63 mmoL H2/g of cellulose), respectively. Biohydrogen production performance obtained from this work is better than most of the reported results from relevant studies. The present study revealed the cost-effective process combining cellulolytic enzymes production under solid state fermentation (SSF) and the conversion of agro-industrial residues into renewable energy resources.
Department of Chemical and Biological Engineering, Korea University, Seongbuk-gu, Seoul, 136-713, South Korea.
Ganesh D
GD
Saratale
Siddheshwar D
SD
Kshirsagar
Vilas T
VT
Sampange
Rijuta G
RG
Saratale
Sang-Eun
SE
Oh
Sanjay P
SP
Govindwar
Min-Kyu
MK
Oh
D000383:Agriculture; D056804:Biofuels; D002480:Cellulase / Q000737:chemistry*; D046970:Clostridium beijerinckii / Q000254:growth & development*; D005656:Fungal Proteins / Q000737:chemistry*; D006026:Glycoside Hydrolases / Q000737:chemistry*; D006859:Hydrogen / Q000378:metabolism*; D017744:Medical Waste Disposal / Q000379:methods; D020075:Phanerochaete / Q000201:enzymology*; D062611:Solid Waste
D056804:Biofuels; D005656:Fungal Proteins; D017744:Medical Waste Disposal; D062611:Solid Waste; D006859:Hydrogen; D006026:Glycoside Hydrolases; C023305:hemicellulase; D002480:Cellulase