Grease matrix as a versatile carrier of proteins for serial crystallography.
Serial femtosecond X-ray crystallography (SFX) has revolutionized atomic-resolution structural investigation by expanding applicability to micrometer-sized protein crystals, even at room temperature, and by enabling dynamics studies. However, reliable crystal-carrying media for SFX are lacking. Here we introduce a grease-matrix carrier for protein microcrystals and obtain the structures of lysozyme, glucose isomerase, thaumatin and fatty acid-binding protein type 3 under ambient conditions at a resolution of or finer than 2 Å.
RIKEN SPring-8 Center, Sayo, Japan.
Michihiro
M
Sugahara
Department of Applied Chemistry, Graduate School of Engineering, Osaka University, Suita, Japan.
Eiichi
E
Mizohata
RIKEN SPring-8 Center, Sayo, Japan.
Eriko
E
Nango
1] RIKEN SPring-8 Center, Sayo, Japan. [2] Laboratory of Supramolecular Crystallography, Research Center for Structural and Functional Proteomics, Institute for Protein Research, Osaka University, Suita, Japan.
Mamoru
M
Suzuki
RIKEN SPring-8 Center, Sayo, Japan.
Tomoyuki
T
Tanaka
1] RIKEN SPring-8 Center, Sayo, Japan. [2] Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University, Uji, Japan.
Tetsuya
T
Masuda
RIKEN SPring-8 Center, Sayo, Japan.
Rie
R
Tanaka
Department of Cell Biology, Graduate School of Medicine, Kyoto University, Sakyo, Japan.
Tatsuro
T
Shimamura
Department of Cell Biology, Graduate School of Medicine, Kyoto University, Sakyo, Japan.
Yoshiki
Y
Tanaka
Department of Cell Biology, Graduate School of Medicine, Kyoto University, Sakyo, Japan.
Chiyo
C
Suno
Department of Cell Biology, Graduate School of Medicine, Kyoto University, Sakyo, Japan.
Kentaro
K
Ihara
Department of Structural Biology, Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo, Japan.
Dongqing
D
Pan
Japan Science and Technology Agency (JST)-Exploratory Research for Advanced Technology (ERATO), Lipid Active Structure Project, Toyonaka, Japan.
Keisuke
K
Kakinouchi
Japan Science and Technology Agency (JST)-Exploratory Research for Advanced Technology (ERATO), Lipid Active Structure Project, Toyonaka, Japan.
Shigeru
S
Sugiyama
Japan Science and Technology Agency (JST)-Exploratory Research for Advanced Technology (ERATO), Lipid Active Structure Project, Toyonaka, Japan.
Michio
M
Murata
Department of Applied Chemistry, Graduate School of Engineering, Osaka University, Suita, Japan.
Tsuyoshi
T
Inoue
Japan Synchrotron Radiation Research Institute, Sayo, Japan.
Kensuke
K
Tono
RIKEN SPring-8 Center, Sayo, Japan.
Changyong
C
Song
RIKEN SPring-8 Center, Sayo, Japan.
Jaehyun
J
Park
Japan Synchrotron Radiation Research Institute, Sayo, Japan.
Takashi
T
Kameshima
RIKEN SPring-8 Center, Sayo, Japan.
Takaki
T
Hatsui
Japan Synchrotron Radiation Research Institute, Sayo, Japan.
Yasumasa
Y
Joti
RIKEN SPring-8 Center, Sayo, Japan.
Makina
0000000224721684
M
Yabashi
1] RIKEN SPring-8 Center, Sayo, Japan. [2] Department of Cell Biology, Graduate School of Medicine, Kyoto University, Sakyo, Japan.
So
S
Iwata
D019747:Aldose-Ketose Isomerases / Q000737:chemistry; D003460:Crystallization; D018360:Crystallography, X-Ray / Q000379:methods*; D000074026:Fatty Acid Binding Protein 3; D050556:Fatty Acid-Binding Proteins / Q000737:chemistry; D007834:Lasers; D054327:Lubricants; D008899:Mineral Oil; D009113:Muramidase / Q000737:chemistry; D010940:Plant Proteins / Q000737:chemistry; D011506:Proteins / Q000737:chemistry*
C493408:FABP3 protein, human; D000074026:Fatty Acid Binding Protein 3; D050556:Fatty Acid-Binding Proteins; D054327:Lubricants; D010940:Plant Proteins; D011506:Proteins; C003427:thaumatin protein, plant; D008899:Mineral Oil; D009113:Muramidase; D019747:Aldose-Ketose Isomerases; C023298:xylose isomerase