A homodimeric BODIPY rotor as a fluorescent viscosity sensor for membrane-mimicking and cellular environments.
Fluorescence properties of a novel homodimeric BODIPY dye rotor for Fluorescence Lifetime Imaging Microscopy (FLIM) are reported. Steady state and time resolved fluorescence measurements established the viscosity dependent behaviour in vitro. Homodimeric BODIPY embedded in different membrane mimicking lipid vesicles (DPPC, POPC and POPC plus cholesterol) is demonstrated to be a viable sensor for fluorescence lifetime based viscosity measurements. Moreover, SKOV3 cells readily endocytosed the dye, which accumulated in membranous structures inside the cytoplasm thereby allowing viscosity mapping of internal cell components.
Department of Physics and Astronomy, Texas Christian University, Fort Worth, TX 76129, USA. z.gryczynski@tcu.edu.
Sangram
S
Raut
Joseph
J
Kimball
Rafal
R
Fudala
Hung
H
Doan
Badri
B
Maliwal
Nirupama
N
Sabnis
Andras
A
Lacko
Ignacy
I
Gryczynski
Sergei V
SV
Dzyuba
Zygmunt
Z
Gryczynski
D001896:Boron Compounds / Q000737:chemistry*; D002460:Cell Line; D002462:Cell Membrane / Q000737:chemistry*; D003593:Cytoplasm / Q000737:chemistry*; D019281:Dimerization; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D008856:Microscopy, Fluorescence; D061848:Optical Imaging; D014783:Viscosity
C095489:4,4-difluoro-4-bora-3a,4a-diaza-s-indacene; D001896:Boron Compounds; D005456:Fluorescent Dyes