The influence of 1α.25-dihydroxyvitamin d3 coating on implant osseointegration in the rabbit tibia.
OBJECTIVE
This study aims to evaluate bone response to an implant surface modified by 1α,25-dihydroxyvitamin D3 [1.25-(OH)2D3] in vivo and the potential link between 1.25-(OH) 2D3 surface concentration and bone response.


METHODS
Twenty-eight implants were divided into 4 groups (1 uncoated control, 3 groups coated with 1.25-(OH)2D3 in concentrations of 10(-8), 10(-7) and 10(-6) M respectively), placed in the rabbit tibia for 6 weeks. Topographical analyses were carried out on coated and uncoated discs using interferometer and atomic-force-microscope (AFM). Twenty-eight implants were histologically observed (bone-to-implant-contact [BIC] and new-bone-area [NBA]).


RESULTS
The results showed that the 1.25-(OH)2D3 coated implants presented a tendency to osseointegrate better than the non-coated surfaces, the differences were not significant (P > 0.05).


CONCLUSIONS
The effect of 1.25-(OH)2D3 coating to implants suggested possible dose dependent effects, however no statistical differences could be found. It is thought that the base substrate topography (turned) could not sustain sufficient amount of 1.25-(OH)2D3 enough to present significant biologic responses. Thus, development a base substrate that can sustain 1.25-(OH)2D3 for a long period is necessary in future studies.
epartment of Prosthodontics, Faculty of Odontology, Malmö University, Malmö Sweden. ; Department of Oral and Maxillofacial Prosthodontics and Oral Implantology, Institute of Health Biosciences, The University of Tokushima Graduate School, Tokushima Japan.
Yoshihito
Y
Naito
epartment of Prosthodontics, Faculty of Odontology, Malmö University, Malmö Sweden. ; Department of Applied Prosthodontics, Graduate School of Biomedical Sciences, Nagasaki University, Nagasaki Japan.
Ryo
R
Jimbo
Department of Restorative Dentistry, West Virginia University School of Dentistry, Morgantown, West Virginia USA.
Matthew S
MS
Bryington
Department of Periodontology and Oral Implantology, Dental School, Faculty of Medicine and Health sciences, University of Ghent Belgium.
Stefan
S
Vandeweghe
epartment of Prosthodontics, Faculty of Odontology, Malmö University, Malmö Sweden.
Bruno R
BR
Chrcanovic
Department of Biomaterials and Biomimetics, New York University, New York USA.
Nick
N
Tovar
epartment of Prosthodontics, Faculty of Odontology, Malmö University, Malmö Sweden.
Tetsuo
T
Ichikawa
Department of Biomaterials and Biomimetics, New York University, New York USA.
Coelho
C
Paulo G
epartment of Prosthodontics, Faculty of Odontology, Malmö University, Malmö Sweden.
Ann
A
Wennerberg
bone formation; dental implants; drug dose response relationship; histological techniques; vitamin D