Clot waveform analysis in patients with haemophilia A.
Clot waveform analysis extends the interpretation of aPTT measurement curves. The curve is mathematically processed to obtain information about fibrin formation kinetics including semiquantitative determination of thrombin, prothrombinase and tenase activity.


METHODS
In this study the feasibility of clot waveform analysis for monitoring of haemophilia A was investigated using blood samples from healthy controls as well as haemophilia A patients under various clinical conditions.


RESULTS
Thrombin, prothrombinase and tenase activity show a high correlation to factor VIII levels. Tenase activity was found to exhibit a linear relationship to factor VIII levels over a very large concentration range and was able to discriminate patients with severe, moderate and mild haemophilia.


CONCLUSIONS
Clot waveform analysis is an easy, fast and cheap method to access disturbances in clot formation and can be done without any additional measurements beside an aPTT.
T. Siegemund, MVZ Lab Dr. Reising-Ackermann and Colleagues, Center of Coagulation Disorders, Strümpellstr. 40, 04289 Leipzig, Germany.
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Siegemund
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Scholz
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Schobess
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Siegemund
D001780:Blood Coagulation Tests / Q000379:methods*; D003627:Data Interpretation, Statistical; D003936:Diagnosis, Computer-Assisted / Q000379:methods*; D005169:Factor VIII / Q000032:analysis* / Q000378:metabolism*; D005240:Feasibility Studies; D005337:Fibrin / Q000032:analysis / Q000378:metabolism*; D006467:Hemophilia A / Q000097:blood* / Q000175:diagnosis*; D006801:Humans; D008657:Metabolic Clearance Rate; D010363:Pattern Recognition, Automated / Q000379:methods; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D005169:Factor VIII; D005337:Fibrin
Activated partial thromboplastin time; fibrin formation kinetics; haemophilia; prothrombinase; tenase; thrombin