Protein profiling of preeclampsia placental tissues.
Preeclampsia is a multi-system disorder involved in pregnancy without an effective treatment except delivery. The precise pathogenesis of this complicated disorder is still not completely understood. The objective of this study is to evaluate the alterations of protein expression and phosphorylations that are important in regulating placental cell function in preterm and term preeclampsia. Using the Protein Pathway Array, 38 proteins in placental tissues were found to be differentially expressed between preterm preeclampsia and gestational age matched control, while 25 proteins were found to be expressed differentially between term preeclampsia and matched controls. Among these proteins, 16 proteins and their associated signaling pathways overlapped between preterm and term preeclampsia, suggesting the common pathogenesis of two subsets of disease. On the other hand, many proteins are uniquely altered in either preterm or term preeclampsia and correlated with severity of clinical symptoms and outcomes, therefore, providing molecular basis for these two subsets of preeclampsia. Furthermore, the expression levels of some of these proteins correlated with neonatal small for gestational age (PAI-1 and PAPP-A) and adverse outcomes (Flt-1) in women with preterm preeclampsia. These proteins could potentially be used as candidate biomarkers for predicting outcomes of preeclampsia.
Department of Obstetrics and Gynecology, The First Hospital of Jilin University, Changchun, Jilin, China.
Chang
C
Shu
Department of Obstetrics and Gynecology, Tufts Medical Center, Tufts University School of Medicine, Boston, Massachusetts, United States of America.
Zitao
Z
Liu
Department of Obstetrics and Gynecology, The First Hospital of Jilin University, Changchun, Jilin, China.
Lifeng
L
Cui
Department of Medicine Bioinformatics Core, Icahn School of Medicine at Mount Sinai, New York, New York, United States of America.
Chengguo
C
Wei
Computer Center of Jilin Province, Changchun, Jilin, China.
Shuwen
S
Wang
Department of Obstetrics, Gynecology and Reproductive Science, Icahn School of Medicine at Mount Sinai, New York, New York, United States of America.
Jian Jenny
JJ
Tang
Department of Pathology, Icahn School of Medicine at Mount Sinai, New York, New York, United States of America.
Miao
M
Cui
Department of Gastrointestinal Surgery, Shandong Provincial Hospital, Jinan, Shandong, China.
Guodong
G
Lian
Department of Gastrointestinal Surgery, The First Hospital of Jilin University, Changchun, Jilin, China.
Wei
W
Li
Department of Ophthalmology, The First Hospital of Jilin University, Changchun, Jilin, China.
Xiufen
X
Liu
Department of Obstetrics and Gynecology, The First Hospital of Jilin University, Changchun, Jilin, China.
Hongmei
H
Xu
Division of Clinical Epidemiology, The First Hospital of Jilin University, Chang chun, Jilin, China.
Jing
J
Jiang
Departments of Pathology, Urology and New York University Cancer Institute, New York University, School of Medicine, New York, New York, United States of America.
Peng
P
Lee
Department of Pathology, Icahn School of Medicine at Mount Sinai, New York, New York, United States of America.
David Y
DY
Zhang
Department of Obstetrics and Gynecology, The First Hospital of Jilin University, Changchun, Jilin, China.
Jin
J
He
Department of Pathology, Icahn School of Medicine at Mount Sinai, New York, New York, United States of America.
Fei
F
Ye
D000328:Adult; D015415:Biomarkers / Q000378:metabolism; D005260:Female; D005865:Gestational Age; D006801:Humans; D010920:Placenta / Q000378:metabolism* / Q000503:physiopathology; D011225:Pre-Eclampsia / Q000175:diagnosis / Q000378:metabolism* / Q000503:physiopathology; D011247:Pregnancy; D011257:Pregnancy Proteins / Q000096:biosynthesis*
D015415:Biomarkers; D011257:Pregnancy Proteins