In vitro Evaluation of Antibacterial Efficacy of Pineapple Extract (Bromelain) on Periodontal Pathogens.
BACKGROUND
Periodontitis is an inflammatory disease resulting in the destruction of periodontal tissues. Various treatment modalities have been tried in the form of mechanical therapy and surgical therapy. Antimicrobial agents have been used as a monotherapy and as an adjunct with mechanical debridement. Various plant extracts have been used as antibacterial agents. Pineapple extract (bromelian) is one such agent. Hence this study was conducted to assess the antibacterial efficacy of bromelain on both aerobic and anaerobic periodontal microorganisms. The aim was to assess the antibacterial efficacy of bromelain on both aerobic and anaerobic periodontal microorganisms.


METHODS
Minimum inhibitory concentration (MIC) of bromelain was tested on isolated strains of Streptococcus mutans, Enterococcus fecalis Aggregatibacter actinomycetemcomitans (Aa), and Porphyromonas gingivalis (Pg) using serial dilution broth method.


RESULTS
S. mutans showed sensitivity at the lowest concentration of 2 mg/ml as compared to E fecalis (31.25 mg/ml) while Pgingivalis showed sensitivity at the lowest concentration of 4.15 mg/ml as compared to Aa (16.6 mg/ml).


CONCLUSIONS
Bromelain exerts an antibacterial effect against potent periodontal pathogens; hence, it may be used as an antibacterial agent. However, further trial has to be conducted to validate this result.
Assistant Professor, Department of Periodontics, College of Dental Sciences, Davangere, Karnataka, India.
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NC
Praveen
Reader, Department of Periodontics, KGF College of Dental Sciences, KGF, Karnataka, India.
A
A
Rajesh
Professor, Department of Pedodontics, MM College of Dental Sciences, Mullana, Haryana, India.
Manish
M
Madan
PG Student, Department of Conservative Dentistry and Endodontics, KLE Dental College, Belgaum, Karnataka, India.
Vishwajit Rampratap
VR
Chaurasia
Assistant Professor, Department of Conservative Dentistry and Endodontics, College of Dental Sciences, Davanagere, Karnataka, India.
Neel V
NV
Hiremath
BDS Student, KLE Dental College, Belgaum, Karnataka, India.
Akanksha Manmohan
AM
Sharma
Aggregatibacter actinomycetemcomitans; Enterococcus fecalis periodontitis; Porphyromonas gingivalis; Streptococcus mutans; bromelain