Conjugates of a photoactivated rhodamine with biopolymers for cell staining.
Conjugates of the photoactivated rhodamine dyes with biopolymers (proteins, polysaccharides, and nucleic acids) are important tools for microscopic investigation of biological tissue. In this study, a precursor of the photoactivated fluorescent dye (PFD) has been successfully used for staining of numerous mammalian cells lines and for conjugate formation with chitosan ("Chitosan-PFD") and histone H1 ("Histone H1.3-PFD"). The intensive fluorescence has been observed after photoactivation of these conjugates inside cells (A431, HaCaT, HEK239, HBL-100, and MDCK). Developed procedures and obtained data are important for further application of novel precursors of fluorescent dyes ("caged" dyes) for microscopic probing of biological objects. Thus, the synthesized "Chitosan-PFD" and "Histone H1-PFD" have been successfully applied in this study for intracellular transport visualization by fluorescent microscopy.
Moscow State Academy of Veterinary Medicine and Biotechnology, Akad. Skryabin Street 23, Moscow 109472, Russia.
Sergei Yu
SY
Zaitsev
Moscow State Academy of Veterinary Medicine and Biotechnology, Akad. Skryabin Street 23, Moscow 109472, Russia.
Mikhail N
MN
Shaposhnikov
Moscow State Academy of Veterinary Medicine and Biotechnology, Akad. Skryabin Street 23, Moscow 109472, Russia.
Daria O
DO
Solovyeva
Kazan Federal University, Kremlevskaya Street 18, Kazan 420008, Russia.
Valeria V
VV
Solovyeva
Kazan Federal University, Kremlevskaya Street 18, Kazan 420008, Russia.
Albert A
AA
Rizvanov
D000818:Animals; D001704:Biopolymers / Q000737:chemistry*; D058948:Cell Tracking; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D057809:HEK293 Cells; D006801:Humans; D008856:Microscopy, Fluorescence; D011506:Proteins / Q000737:chemistry; D012235:Rhodamines / Q000138:chemical synthesis* / Q000737:chemistry; D013194:Staining and Labeling
D001704:Biopolymers; D005456:Fluorescent Dyes; D011506:Proteins; D012235:Rhodamines