Water-soluble colorimetric and ratiometric fluorescent probe for selective imaging of palladium species in living cells.
A novel water-soluble colorimetric and ratiometric fluorescent probe was synthesized and applied to imaging palladium species under physiological conditions in phosphate buffered saline (PBS) containing less than 1% organic cosolvent without adding any additional reagents. Based on palladium triggered terminal propargyl ethers cleavage reaction, the probe exhibited a high selectivity and sensitivity for palladium species of all the typical oxidation states (0, +2, +4), with a low detection limit (25 nM, 2.7 μg/L) and an obvious color change. Furthermore, the probe was successfully used for ratiometric fluorescence imaging of palladium in living cells.
Key Laboratory of Nonferrous Metals Chemistry and Resources Utilization of Gansu Province, State Key Laboratory of Applied Organic Chemistry and College of Chemistry and Chemical Engineering, Lanzhou University , Lanzhou 730000, China.
Wei
W
Liu
Jie
J
Jiang
Chunyang
C
Chen
Xiaoliang
X
Tang
Jinmin
J
Shi
Peng
P
Zhang
Kaiming
K
Zhang
Zhiqi
Z
Li
Wei
W
Dou
Lizi
L
Yang
Weisheng
W
Liu
D003124:Colorimetry / Q000379:methods*; D005456:Fluorescent Dyes / Q000138:chemical synthesis* / Q000737:chemistry; D056945:Hep G2 Cells; D006801:Humans; D015394:Molecular Structure; D010165:Palladium / Q000737:chemistry*; D014867:Water / Q000737:chemistry*
D005456:Fluorescent Dyes; D014867:Water; D010165:Palladium