Near-infrared dye-conjugated amphiphilic hyaluronic acid derivatives as a dual contrast agent for in vivo optical and photoacoustic tumor imaging.
Amphiphilic hyaluronic acid (HA) derivatives bearing hydrophobic indocyanine green dye derivatives and hydrophilic poly(ethylene glycol) were synthesized through the use of condensation and copper-catalyzed click cyclization reactions. The amphiphilic HA derivatives dissolved in water and formed self-assemblies in which the near-infrared dyes were tightly packed and arranged to form dimers or H-aggregates. By irradiating an aqueous solution of HA derivatives with near-infrared light, photoacoustic signals were detected along with fluorescence emission. Self-assemblies consisting of HA derivatives could smoothly accumulate in tumor tissues by passive tumor targeting. By utilizing HA derivatives as a contrast agent, tumor sites were clearly visualized by optical imaging as well as by photoacoustic tomography.
Department of Energy and Hydrocarbon Chemistry and ‡Department of Polymer Chemistry, Graduate School of Engineering, Kyoto University , Nishikyo-ku, Kyoto 615-8510, Japan.
Koji
K
Miki
Tatsuhiro
T
Inoue
Yasuhito
Y
Kobayashi
Katsuya
K
Nakano
Hideki
H
Matsuoka
Fumio
F
Yamauchi
Tetsuya
T
Yano
Kouichi
K
Ohe
D000276:Adjuvants, Immunologic / Q000138:chemical synthesis; D000818:Animals; D045744:Cell Line, Tumor; D003110:Colonic Neoplasms / Q000473:pathology*; D004396:Coloring Agents / Q000737:chemistry; D003287:Contrast Media / Q000138:chemical synthesis; D006820:Hyaluronic Acid / Q000031:analogs & derivatives*; D007208:Indocyanine Green / Q000737:chemistry; D007259:Infrared Rays; D051379:Mice; D009368:Neoplasm Transplantation; D061088:Photoacoustic Techniques; D011092:Polyethylene Glycols / Q000737:chemistry; D014054:Tomography
D000276:Adjuvants, Immunologic; D004396:Coloring Agents; D003287:Contrast Media; D011092:Polyethylene Glycols; D006820:Hyaluronic Acid; D007208:Indocyanine Green