Characterization and quantification of γ-oryzanol in grains of 16 Korean rice varieties.
γ-Oryzanol, a mixture of ferulic acid esters of triterpene alcohols and sterols, is a nutritionally important group of rice secondary metabolites. A library of 27 γ-oryzanol was assembled from existing data and used to assist identification and quantification of γ-oryzanol isolated from 16 Korean rice varieties (11 white and 5 pigmented). γ-Oryzanol was analyzed with liquid chromatography with diode array detection and electrospray ionization mass spectrometry. Nineteen different γ-oryzanol were observed and identified as stigmasterol, campesterol and sitosterol or common and hydroxylated triterpene alcohols. In the 16 varieties, the total γ-oryzanol content averaged 43.8 mg/100 g (range, 26.7-61.6 mg/100 g), which Josaengheugchal exhibited the highest level (61.6 mg/100 g). The Korean rice varieties were classified based on qualitative and quantitative γ-oryzanol data by multivariate statistical analysis. Clusters of specialty rice varieties exhibited higher γ-oryzanol levels than those of common rice varieties.
Department of Agro-Food Resources, National Academy of Agricultural Science, Rural Development Administration , Suwon , Republic of Korea and.
Heon Woong
HW
Kim
Jung Bong
JB
Kim
Soo-Muk
SM
Cho
Il Kyu
IK
Cho
Qing X
QX
Li
Hwan-Hee
HH
Jang
Sung-Hyeon
SH
Lee
Young-Min
YM
Lee
Kyung-A
KA
Hwang
D002784:Cholesterol / Q000031:analogs & derivatives / Q000032:analysis; D002853:Chromatography, Liquid; D003373:Coumaric Acids / Q000032:analysis; D006801:Humans; D007723:Korea; D012275:Oryza / Q000737:chemistry* / Q000145:classification; D010666:Phenylpropionates / Q000032:analysis*; D010840:Phytosterols / Q000032:analysis*; D012639:Seeds / Q000737:chemistry*; D013045:Species Specificity; D021241:Spectrometry, Mass, Electrospray Ionization; D014315:Triterpenes / Q000032:analysis
D003373:Coumaric Acids; D010666:Phenylpropionates; D010840:Phytosterols; D014315:Triterpenes; C021273:campesterol; D002784:Cholesterol; C004999:ferulic acid; C013172:gamma-oryzanol
ferulate; ferulic acid; functional food; mass spectrometry; rice; γ-Oryzanol