Functional classification of BRCA2 DNA variants by splicing assays in a large minigene with 9 exons.
Numerous pathogenic DNA variants impair the splicing mechanism in human genetic diseases. Minigenes are optimal approaches to test variants under the splicing viewpoint without the need of patient samples. We aimed to design a robust minigene construct of the breast cancer gene BRCA2 in order to investigate the impact of variants on splicing. BRCA2 exons 19-27 (MGBR2_ex19-27) were cloned in the new vector pSAD. It produced a large transcript of the expected size (2,174 nucleotides) and exon structure (V1-ex19-27-V2). Splicing assays showed that 18 (17 splice-site and 1 silencer variants) out of 40 candidate DNA variants induced aberrant patterns. Twenty-four anomalous transcripts were accurately detected by fluorescent-RT-PCR that were generated by exon-skipping, alternative site usage, and intron-retention events. Fourteen variants induced major anomalies and were predicted to disrupt protein function so they could be classified as pathogenic. Furthermore, minigene mimicked previously reported patient RNA outcomes of seven variants supporting the reproducibility of minigene assays. Therefore, a relevant fraction of variants are involved in breast cancer through splicing alterations. MGBR2_ex19-27 is the largest reported BRCA2 minigene and constitutes a valuable tool for the functional and clinical classification of sequence variations.
Splicing and Genetic Susceptibility to Cancer, Instituto de Biología y Genética Molecular (CSIC-UVa), Valladolid, Spain.
Alberto
A
Acedo
Cristina
C
Hernández-Moro
Álvaro
Á
Curiel-García
Beatriz
B
Díez-Gómez
Eladio A
EA
Velasco
D017398:Alternative Splicing; D024682:BRCA2 Protein / Q000235:genetics* / Q000378:metabolism; D005091:Exons; D056726:Genetic Association Studies; D006367:HeLa Cells; D061325:Hereditary Breast and Ovarian Cancer Syndrome / Q000235:genetics; D006801:Humans; D061986:MCF-7 Cells; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism
D024682:BRCA2 Protein; C551750:BRCA2 protein, human; D020033:Protein Isoforms
BRCA2; hereditary breast and ovarian cancer; minigenes; splicing; unclassified variants