Novel cationic triblock copolymer of poly[2-(dimethylamino)ethyl methacrylate]-block-poly(β-amino ester)-block-poly[2-(dimethylamino)ethyl methacrylate]: a promising non-viral gene delivery system.
This manuscript reports the synthesis of a new cationic block copolymer based on poly[2-(dimethylamino)ethyl methacrylate] and poly(β-amino ester) from different polymerization strategies. For the first time, it is proposed a triblock copolymer based only on cationic segments, aiming a high biocompatibility, enhanced buffering capacity and stimuli-responsive character in a single structure. The new block copolymer successfully condensed the plasmid DNA into nanosized polyplexes. The polyplexes were tested in two different cell lines revealing ∼4-fold and ∼6-fold (in HeLa cells), and ∼11-fold (in COS-7 cells) higher transgene expression than branched PEI and TurboFect™, respectively. These results show that this new block copolymer is a promising candidate to be used as a polymeric non-viral vector.
Department of Chemical Engineering, University of Coimbra, Polo II, Rua Sílvio Lima, 3030-790, Coimbra, Portugal.
Rosemeyre A
RA
Cordeiro
Dina
D
Farinha
Nuno
N
Rocha
Arménio C
AC
Serra
Henrique
H
Faneca
Jorge F J
JF
Coelho
D000818:Animals; D019556:COS Cells; D002522:Chlorocebus aethiops; D002850:Chromatography, Gel; D057930:Click Chemistry / Q000379:methods*; D004587:Electrophoresis, Agar Gel; D018014:Gene Transfer Techniques / Q000295:instrumentation*; D006367:HeLa Cells; D006801:Humans; D046911:Macromolecular Substances / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008689:Methacrylates / Q000737:chemistry*; D018613:Microscopy, Confocal; D009757:Nylons / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013054:Spectrophotometry, Atomic; D017550:Spectroscopy, Fourier Transform Infrared; D013818:Thermogravimetry
D046911:Macromolecular Substances; D008689:Methacrylates; D009757:Nylons; D011108:Polymers; C407037:poly(2-(dimethylamino)ethyl methacrylate); C507253:poly(beta-amino ester)
cationic block copolymer; gene delivery; poly(β-amino ester); poly[2-(dimethylamino)ethyl methacrylate]; “click” chemistry