Next generation semiconductor based-sequencing of a nutrigenetics target gene (GPR120) and association with growth rate in Italian Large White pigs.
The GPR120 gene (also known as FFAR4 or O3FAR1) encodes for a functional omega-3 fatty acid receptor/sensor that mediates potent insulin sensitizing effects by repressing macrophage-induced tissue inflammation. For its functional role, GPR120 could be considered a potential target gene in animal nutrigenetics. In this work we resequenced the porcine GPR120 gene by high throughput Ion Torrent semiconductor sequencing of amplified fragments obtained from 8 DNA pools derived, on the whole, from 153 pigs of different breeds/populations (two Italian Large White pools, Italian Duroc, Italian Landrace, Casertana, Pietrain, Meishan, and wild boars). Three single nucleotide polymorphisms (SNPs), two synonymous substitutions and one in the putative 3'-untranslated region (g.114765469C > T), were identified and their allele frequencies were estimated by sequencing reads count. The g.114765469C > T SNP was also genotyped by PCR-RFLP confirming estimated frequency in Italian Large White pools. Then, this SNP was analyzed in two Italian Large White cohorts using a selective genotyping approach based on extreme and divergent pigs for back fat thickness (BFT) estimated breeding value (EBV) and average daily gain (ADG) EBV. Significant differences of allele and genotype frequencies distribution was observed between the extreme ADG-EBV groups (P < 0.001) whereas this marker was not associated with BFT-EBV.
a Department of Agricultural and Food Sciences (DISTAL), Division of Animal Sciences , University of Bologna , Bologna , Italy.
Luca
L
Fontanesi
Francesca
F
Bertolini
Emilio
E
Scotti
Giuseppina
G
Schiavo
Michela
M
Colombo
Paolo
P
Trevisi
Anisa
A
Ribani
Luca
L
Buttazzoni
Vincenzo
V
Russo
Stefania
S
Dall'Olio
D000818:Animals; D005787:Gene Frequency; D056726:Genetic Association Studies; D007558:Italy; D054647:Nutrigenomics / Q000379:methods*; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D043562:Receptors, G-Protein-Coupled / Q000235:genetics*; D012666:Semiconductors; D017422:Sequence Analysis, DNA / Q000295:instrumentation / Q000379:methods*; D034421:Sus scrofa / Q000235:genetics* / Q000254:growth & development*
D043562:Receptors, G-Protein-Coupled
Association study; GPR120; Heavy pigs; Ion Torrent semiconductor sequencing; SNP