Simultaneous quantification of Hg²⁺ and MeHg⁺ in aqueous media with a single fluorescent probe by multiplexing in the time domain.
Development of a molecular probe for selective detection of MeHg(+) in the presence of Hg(2+) is a mission impossible to accomplish. Speciation analysis of two substrates with a single kinetic trace exploiting their differential reactivity toward a single probe, i.e., multiplexing in the time domain, is a cost-effective and powerful alternative. We have developed such a probe (Hg410) for simultaneously quantification of Hg(2+) and MeHg(+) in aqueous media. Hg410 is designed via the "covalent-assembly" approach, displays a zero background, and bears a very concise molecular construct. It has harnessed proximity-based catalysis to achieve high reactivity toward Hg(2+) and MeHg(+). An unprecedentedly low detection limit of ca. 4.6 pM and 160 pM was measured for Hg(2+) and MeHg(+), respectively.
State Key Laboratory of Bioreactor Engineering, and ‡Shanghai Key Laboratory of Chemical Biology, School of Pharmacy, East China University of Science and Technology , Shanghai, 200237 China.
Ziqian
Z
Zhang
Baoyan
B
Zhang
Xuhong
X
Qian
Zhong
Z
Li
Zhiping
Z
Xu
Youjun
Y
Yang
D005456:Fluorescent Dyes / Q000737:chemistry*; D008628:Mercury / Q000032:analysis*; D008767:Methylmercury Compounds / Q000032:analysis*; D008968:Molecular Conformation; D013997:Time Factors; D014867:Water / Q000737:chemistry*
D005456:Fluorescent Dyes; D008767:Methylmercury Compounds; D014867:Water; D008628:Mercury