Phenotypic and proteomic characteristics of human dental pulp derived mesenchymal stem cells from a natal, an exfoliated deciduous, and an impacted third molar tooth.
The level of heterogeneity among the isolated stem cells makes them less valuable for clinical use. The purpose of this study was to understand the level of heterogeneity among human dental pulp derived mesenchymal stem cells by using basic cell biology and proteomic approaches. The cells were isolated from a natal (NDPSCs), an exfoliated deciduous (stem cells from human exfoliated deciduous (SHED)), and an impacted third molar (DPSCs) tooth of three different donors. All three stem cells displayed similar features related to morphology, proliferation rates, expression of various cell surface markers, and differentiation potentials into adipocytes, osteocytes, and chondrocytes. Furthermore, using 2DE approach coupled with MALDI-TOF/TOF, we have generated a common 2DE profile for all three stem cells. We found that 62.3 ± 7% of the protein spots were conserved among the three mesenchymal stem cell lines. Sixty-one of these conserved spots were identified by MALDI-TOF/TOF analysis. Classification of the identified proteins based on biological function revealed that structurally important proteins and proteins that are involved in protein folding machinery are predominantly expressed by all three stem cell lines. Some of these proteins may hold importance in understanding specific properties of human dental pulp derived mesenchymal stem cells.
DEKART Proteomics Laboratory, Kocaeli University Medical School, Umuttepe, 41380 Kocaeli, Turkey.
Gurler
G
Akpinar
DEKART Proteomics Laboratory, Kocaeli University Medical School, Umuttepe, 41380 Kocaeli, Turkey ; Department of Medical Biology, Kocaeli University Medical School, Umuttepe, 41380 Kocaeli, Turkey.
Murat
0000-0001-8527-2096
M
Kasap
Department of Stem Cell, Center for Stem Cell and Gene Therapies Research and Practice, Institute of Health Sciences, Umuttepe, 41380 Kocaeli, Turkey.
Ayca
A
Aksoy
Department of Stem Cell, Center for Stem Cell and Gene Therapies Research and Practice, Institute of Health Sciences, Umuttepe, 41380 Kocaeli, Turkey.
Gokhan
G
Duruksu
Department of Stem Cell, Center for Stem Cell and Gene Therapies Research and Practice, Institute of Health Sciences, Umuttepe, 41380 Kocaeli, Turkey.
Gulcin
G
Gacar
Department of Stem Cell, Center for Stem Cell and Gene Therapies Research and Practice, Institute of Health Sciences, Umuttepe, 41380 Kocaeli, Turkey ; Liv Hospital, Center for Regenerative Medicine and Stem Cell Research & Manufacturing (Liv MedCell), Besiktas, 34340 Istanbul, Turkey.
Erdal
E
Karaoz