Protein profiling the differences between diabetic and normal mouse cumulus cells.
As the number of young people suffering from diabetes increases worldwide, the impact of this disease on human reproduction urgently needs to be addressed. Here we compared the proteomes of cumulus cells of super-ovulated cumulus-oocyte complexes from diabetic and normal mice. We identified 57 up-regulated and 74 down-regulated proteins in diabetic cumulus cells; among these groups were proteins associated with cell cycle, cellular communication, epigenetic regulation, protein localization, and chromatin organization - all in accordance with type I diabetes. The poor-quality follicles derived from diabetic mice were further enforced by the presence of glycoproteins that are specifically expressed by the oocyte or oviductal epithelial cells in the cumulus-cell samples. In conclusion, the proteomic differences between diabetic and normal cumulus cells provide targets for improving the reproduction health of type I diabetic patients.
Diabetes Research Center, Beijing University of Chinese Medicine, Beijing, China.
Guang-Jian
GJ
Jiang
Jun-Yu
JY
Ma
Guang-Li
GL
Zhang
Fang-Fang
FF
Mo
Dong-Wei
DW
Zhang
Si-Hua
SH
Gao
Xiu-Li
XL
Li
D000818:Animals; D015153:Blotting, Western; D054885:Cumulus Cells / Q000166:cytology* / Q000378:metabolism*; D003922:Diabetes Mellitus, Type 1 / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000235:genetics*; D006023:Glycoproteins / Q000378:metabolism; D051379:Mice; D060066:Protein Interaction Maps; D020543:Proteome; D040901:Proteomics; D012098:Reproduction / Q000235:genetics*
D006023:Glycoproteins; D020543:Proteome