A photostable near-infrared fluorescent tracker with pH-independent specificity to lysosomes for long time and multicolor imaging.
A new boron-dipyrromethene-based lysosome tracker, Lyso-NIR, is facilely synthesized. Besides the intensive near-infrared (NIR) fluorescence and high photostability, Lyso-NIR shows the capability to stably localize in lysosomes, which is independent of the local pH. Lyso-NIR does not have the problematic alkalization effect suffered by the commonly used lysotrackers; thus, it shows ignorable cytotoxicity and slightly affects normal physiological functions of lysosomes. The above advantages of Lyso-NIR make it feasible to track lysosomes' dynamic changes in a relatively long time during the full cellular processes such as apoptosis, heavy metal stimulation, and endocytosis, as is demonstrated in this work. Moreover, Lyso-NIR's narrow NIR emission at 740 nm with a full width at half-maximum smaller than 50 nm makes it easy to avoid the crosstalk with the emissions from other common fluorescent probes, which strengthens Lyso-NIR's competitiveness as a standard lysosome tracker for multicolor bioimaging.
State Key Laboratory of Fine Chemicals and School of Life Science and Technology, Dalian University of Technology , 2 Linggong Road, Dalian 116024, P. R. China.
Xinfu
X
Zhang
Chao
C
Wang
Zhuo
Z
Han
Yi
Y
Xiao
D001895:Boron / Q000737:chemistry; D058948:Cell Tracking; D003952:Diagnostic Imaging; D005456:Fluorescent Dyes / Q000737:chemistry; D006801:Humans; D008247:Lysosomes / Q000473:pathology / Q000648:ultrastructure*; D011162:Porphobilinogen / Q000031:analogs & derivatives / Q000737:chemistry; D019265:Spectroscopy, Near-Infrared
D005456:Fluorescent Dyes; C507544:dipyrromethene; D011162:Porphobilinogen; D001895:Boron
fluorescence imaging; lysosome; near-infrared; probe