Bacillus cereus AR156-induced resistance to Colletotrichum acutatum is associated with priming of defense responses in loquat fruit.
The effectiveness of a biocontrol agent Bacillus cereus AR156 for control of anthracnose rot caused by Colletotrichum acutatum in harvested loquat fruit and the possible mechanisms of its action have been investigated. Treatment of fruit with B. cereus AR156 resulted in lower disease incidence and smaller lesion diameters compared with that of untreated fruit. The treatment enhanced activities of defense-related enzymes including chitinase, β-1, 3-glucanase, phenylalanine ammonia-lyase, peroxidase and polyphenoloxidase, and promoted accumulation of H2O2. Total phenolic content and 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity were also increased by treatment. Transcripts of three defense-related genes were enhanced only in fruit undergoing both B. cereus AR156 treatment and C. acutatum inoculation compared with those receiving either intervention alone. These results suggest that the disease resistance against C. acutatum in loquat fruit is enhanced by B. cereus AR156 and that the induced resistance is associated with induction and priming of defense responses in the fruit.
College of Food Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu, China; College of Life Science and Chemical Engineering, Huaiyin Institute of Technology, Huai'an, Jiangsu, China.
Xiaoli
X
Wang
College of Food Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu, China.
Lei
L
Wang
College of Food Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu, China.
Jing
J
Wang
College of Food Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu, China.
Peng
P
Jin
College of Plant Protection, Nanjing Agricultural University, Nanjing, Jiangsu, China.
Hongxia
H
Liu
College of Food Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu, China.
Yonghua
Y
Zheng
D001409:Bacillus cereus / Q000235:genetics*; D061046:Biological Control Agents; D020231:Colletotrichum; D060467:Disease Resistance; D031983:Eriobotrya / Q000235:genetics / Q000276:immunology / Q000382:microbiology*; D005638:Fruit / Q000235:genetics / Q000276:immunology / Q000382:microbiology
D061046:Biological Control Agents