Measurement of nitric oxide in mast cells with the fluorescent indicator DAF-FM diacetate.
The production of nitric oxide in mast cells has been difficult to measure due to the low amounts made by mast cells, as well as limitations in the specificity and sensitivity of the assays available. We present here a sensitive and specific 96-well plate-based method to directly measure NO using the cell-permeable fluorescent compound DAF-FM diacetate.
Pulmonary Research Group, Department of Medicine, University of Alberta, 116th Street & 87 Avenue, Edmonton, AB, Canada, T6G 2R3.
Chris D
CD
St Laurent
Tae Chul
TC
Moon
A Dean
AD
Befus
D000818:Animals; D002460:Cell Line; D005452:Fluoresceins / Q000378:metabolism*; D005456:Fluorescent Dyes / Q000378:metabolism*; D006801:Humans; D008407:Mast Cells / Q000378:metabolism*; D051379:Mice; D009569:Nitric Oxide / Q000378:metabolism*; D013050:Spectrometry, Fluorescence / Q000379:methods*
C503301:4-amino-5-methylamino-2',7'-difluorofluorescein diacetate; D005452:Fluoresceins; D005456:Fluorescent Dyes; D009569:Nitric Oxide