High-quality RNA extracted from biopsied samples dehydrated and stored dried at room temperature without chemical preservation for up to 3 months as evidenced by RT-PCR results.
Handling and maintenance of biological tissues for nucleic acid and/or protein analysis has long been a challenge because of the perceived instability of these molecules at room temperature if not preserved or processed. Structural damage and compromised integrity of aforementioned biomolecules subsequent to preservation have also posed difficulties in their use in research. The development of technologies employing nonfixative methods with the capability to store at room temperature have been of growing interest. Our previous publication exploring preservation of proteins by desiccation challenged the convention of their unstable nature. Herein, we report the results of quantitative and qualitative analyses of RNA from tissue samples that were desiccated and stored at room temperature for up to 3 months. Our results indicate that viable RNA can be obtained from dehydrated ex vivo tissue samples that have been stored at room temperature.
*Department of Science, Concordia University, St. Paul, MN †Department of Pathology, USC Keck School of Medicine, Los Angeles, CA.
Theodore R
TR
Sadler
Ani C
AC
Khodavirdi
D000199:Actins / Q000032:analysis; D000818:Animals; D030161:Avian Proteins / Q000032:analysis; D002122:Calcium Chloride / Q000737:chemistry; D002645:Chickens; D003890:Desiccation / Q000379:methods*; D024601:Glyceraldehyde 3-Phosphate Dehydrogenase (NADP+) / Q000032:analysis; D058427:Hygroscopic Agents / Q000737:chemistry; D008099:Liver / Q000737:chemistry; D011309:Preservation, Biological / Q000379:methods*; D012313:RNA / Q000032:analysis*; D020871:RNA Stability; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013048:Specimen Handling / Q000295:instrumentation / Q000379:methods; D013696:Temperature
D000199:Actins; D030161:Avian Proteins; D058427:Hygroscopic Agents; D012313:RNA; D024601:Glyceraldehyde 3-Phosphate Dehydrogenase (NADP+); D002122:Calcium Chloride