Milk fermented with a 15-lipoxygenase-1-producing Lactococcus lactis alleviates symptoms of colitis in a murine model.
Inflammatory bowel diseases (IBD), such as Crohn's disease and ulcerative colitis, is characterized by extensive inflammation due to dysregulation of the innate and adaptive immune system whose exact etiology is not yet completely understood. Currently there is no cure for IBD, thus the search for new molecules capable of controlling IBD and their delivery to the site of inflammation are the goal of many researchers. The aim of this work was to evaluate the anti-inflammatory effect of the administration of milks fermented by a Lactococcus (L.) lactis strain producing 15-lipoxygenase-1 (15-LOX-1) using a trinitrobenzenesulfonic acid-induced IBD mouse model. The results obtained demonstrated that 15-LOX-1 producing L. lactis was effective in the prevention of the intestinal damage associated to inflammatory bowel disease in a murine model. The work also confirmed previous studies showing that fermented milk is an effective form of administration of recombinant lactic acid bacteria expressing beneficial molecules.
Tessalia D L
TD
Saraiva
Katia
K
Morais
Vanessa B
VB
Pereira
Marcela
M
de Azevedo
Clarissa S
CS
Rocha
Camila C
CC
Prosperi
Ana C
AC
Gomes-Santos
Luis
L
Bermudez-Humaran
Ana M C
AM
Faria
Herve M
HM
Blottiere
Philippe
P
Langella
Anderson
A
Miyoshi
Alejandra de Moreno
Ade M
de LeBlanc
Jean G
JG
LeBlanc
Laboratorio de Genetica Celular e Molecular (LGCM), Instituto de Ciencias Biologicas, Universidade Federal de Minas Gerais (ICB/UFMG), CP 486 CEP 31270-901, Belo Horizonte- MG, Brasil. vasco@icb.ufmg.br.
Vasco
V
Azevedo
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000627:therapeutic use*; D001093:Arachidonate 15-Lipoxygenase / Q000235:genetics / Q000378:metabolism*; D003092:Colitis / Q000139:chemically induced / Q000188:drug therapy*; D004195:Disease Models, Animal; D005260:Female; D005285:Fermentation; D013294:Lactococcus lactis / Q000201:enzymology*; D008807:Mice, Inbred BALB C; D008892:Milk; D014302:Trinitrobenzenesulfonic Acid
D000893:Anti-Inflammatory Agents; D014302:Trinitrobenzenesulfonic Acid; C515716:ALOX15 protein, human; D001093:Arachidonate 15-Lipoxygenase