Whole-genome sequencing confirms that Burkholderia pseudomallei multilocus sequence types common to both Cambodia and Australia are due to homoplasy.
Burkholderia pseudomallei isolates with shared multilocus sequence types (STs) have not been isolated from different continents. We identified two STs shared between Australia and Cambodia. Whole-genome analysis revealed substantial diversity within STs, correctly identified the Asian or Australian origin, and confirmed that these shared STs were due to homoplasy.
Department of Clinical Sciences, Institute of Tropical Medicine, Antwerp, Belgium Laboratory of Microbiology, Faculty of Sciences, Ghent University, Ghent, Belgium.
Birgit
B
De Smet
Global and Tropical Health Division, Menzies School of Health Research, Darwin, Australia derek.sarovich@menzies.edu.au.
Derek S
http://orcid.org/0000-0002-6944-3980
DS
Sarovich
Global and Tropical Health Division, Menzies School of Health Research, Darwin, Australia.
Erin P
http://orcid.org/0000-0002-1079-4882
EP
Price
Global and Tropical Health Division, Menzies School of Health Research, Darwin, Australia.
Mark
M
Mayo
Global and Tropical Health Division, Menzies School of Health Research, Darwin, Australia.
Vanessa
V
Theobald
Sihanouk Hospital Centre of HOPE, Phnom Penh, Cambodia.
Chun
C
Kham
Institut Pasteur du Cambodge, Phnom Penh, Cambodia.
Seiha
S
Heng
Sihanouk Hospital Centre of HOPE, Phnom Penh, Cambodia.
Phe
P
Thong
Wellcome Trust Sanger Institute, Cambridge, United Kingdom University of St. Andrews, St. Andrews, United Kingdom.
Matthew T G
MT
Holden
Wellcome Trust Sanger Institute, Cambridge, United Kingdom.
Julian
http://orcid.org/0000-0002-7069-5958
J
Parkhill
Wellcome Trust Sanger Institute, Cambridge, United Kingdom University of Cambridge, Cambridge, United Kingdom.
Sharon J
SJ
Peacock
Imperial College, London, United Kingdom.
Brian G
BG
Spratt
Department of Clinical Sciences, Institute of Tropical Medicine, Antwerp, Belgium Department of Microbiology and Immunology, University of Leuven, Leuven, Belgium.
Jan A
JA
Jacobs
Laboratory of Microbiology, Faculty of Sciences, Ghent University, Ghent, Belgium.
Peter
P
Vandamme
Global and Tropical Health Division, Menzies School of Health Research, Darwin, Australia.
Bart J
BJ
Currie
D001315:Australia; D015373:Bacterial Typing Techniques; D016957:Burkholderia pseudomallei / Q000235:genetics*; D002160:Cambodia; D019143:Evolution, Molecular; D016680:Genome, Bacterial / Q000235:genetics; D006801:Humans; D008554:Melioidosis / Q000382:microbiology*; D058885:Multilocus Sequence Typing; D010802:Phylogeny