Increased susceptibility to apoptosis and growth arrest of human breast cancer cells treated by a snake venom-loaded silica nanoparticles.
BACKGROUND
The development of effective treatments against metastatic cancers, including breast cancer, is among the most important challenges in current experimental and clinical cancer research. We recently demonstrated that Walterinnesia aegyptia venom (WEV), either alone or in combination with silica nanoparticles (WEV+NP), resulted in the growth arrest and apoptosis of different cancer cell lines.


OBJECTIVE
In the present study, we evaluated the impact of WEV alone and WEV+NP on human breast cancer cells isolated from cancer biopsies.


METHODS
The potential effects of WEV alone and WEV+NP on the proliferation, induction of apoptosis and generation of free radicals in breast cancer cells isolated from 80 patients clinically diagnosed with breast cancer were evaluated by flow cytometry and ELISA.


RESULTS
WEV alone and WEV+NP inhibited the proliferation, altered the cell cycle and enhanced the induction of apoptosis of the breast cancer cells by increasing the activities of caspase-3, caspase-8 and caspase-9. In addition, the combination of WEV and NP robustly sensitized the breast cancer cells to growth arrest and apoptosis by increasing the generation of free radicals, including reactive oxygen species (ROS), hydroperoxide and nitric oxide. The combination of WEV with NP significantly enhanced the anti-tumor effect of WEV in breast cancer cells.


CONCLUSIONS
Our data indicate the therapeutic potential of the nanoparticle-sustained delivery of snake venom for the treatment of breast cancer.
Laboratory of Immunology & Molecular Biology, Zoology Department, Faculty of Science, Assiut University, Assiut, Egypt.
Gamal
G
Badr
Douaa
D
Sayed
Doaa
D
Maximous
Amany O
AO
Mohamed
Mustafa
M
Gul
D017209:Apoptosis / Q000187:drug effects*; D001943:Breast Neoplasms / Q000188:drug therapy* / Q000378:metabolism; D020169:Caspases / Q000378:metabolism; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D005260:Female; D006801:Humans; D053758:Nanoparticles / Q000008:administration & dosage*; D017382:Reactive Oxygen Species / Q000378:metabolism; D012822:Silicon Dioxide / Q000008:administration & dosage*; D012910:Snake Venoms / Q000494:pharmacology*
D017382:Reactive Oxygen Species; D012910:Snake Venoms; D012822:Silicon Dioxide; D020169:Caspases