RT-PCR testing of allograft musculoskeletal tissue: is it time for culture-based methods to move over?
Allograft musculoskeletal tissue samples are assessed for microbial bioburden to reduce the risk of post-transplant infection. Traditionally, solid agar and broth culture media have been used however, nucleic acid testing, such as real-time (RT) polymerase chain reaction (PCR), has been described as more sensitive. This study evaluated the recovery of low numbers of challenge organisms from inoculated swab and musculoskeletal biopsy samples using solid agar culture, cooked meat medium, blood culture bottles and a RT-PCR assay. It was found that broth culture methods were the most sensitive with RT-PCR unable to detect low numbers of bacteria from these samples. Investigation of other non-culture methods may be worthwhile.
South Eastern Area Laboratory Services (SEALS), St George Hospital, Sydney, NSW, Australia.
Kerry
K
Varettas
D000362:Agar / Q000302:isolation & purification; D064591:Allografts; D001419:Bacteria / Q000302:isolation & purification*; D003470:Culture Media; D006801:Humans; D018482:Muscle, Skeletal / Q000473:pathology / Q000637:transplantation*; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D013048:Specimen Handling
D003470:Culture Media; D000362:Agar