Simultaneous ionization and analysis of 84 anabolic androgenic steroids in human urine using liquid chromatography-silver ion coordination ionspray/triple-quadrupole mass spectrometry.
Metal ion coordination ionspray (M(+) CIS) ionization is a powerful technique to enhance ionization efficiency and sensitivity. In this study, we developed and validated an analytical method for simultaneous ionization and analysis of 84 anabolic androgenic steroids (65 exogenous and 19 endogenous) using liquid chromatography-silver ion coordination ionspray/triple-quadrupole mass spectrometry (LC-Ag(+) CIS/MS/MS). The concentrations of silver ions and organic solvents have been optimized to increase the amount of silver ion coordinated complexes. A combination of 25 μM of silver ions and methanol showed the best sensitivity. The validation results showed the intra- (0.8-9.2%) and inter-day (2.5-14.9%) precisions, limits of detection (0.0005-5.0 ng/mL), and matrix effect (71.8-100.3%) for the screening analysis. No significant ion suppression was observed. In addition, this method was successfully applied to analysis of positive samples from suspected abusers and useful for the detection of the trace levels of anabolic steroids in human urine samples.
Doping Control Center, Korea Institute of Science and Technology, Hwarang-ro 14-gil 5, Seongbuk-gu, Seoul, 136-791, Korea; Department of Chemistry, Research Institute for Natural Sciences, Korea University, 145, Anam-ro, Seongbuk-gu, Seoul, 136-701, Korea.
So-Hee
SH
Kim
Eun-Ju
EJ
Cha
Kang Mi
KM
Lee
Ho Jun
HJ
Kim
Oh-Seung
OS
Kwon
Jaeick
J
Lee
D045930:Anabolic Agents / Q000652:urine*; D000728:Androgens / Q000652:urine*; D002851:Chromatography, High Pressure Liquid; D004300:Doping in Sports; D006801:Humans; D012015:Reference Standards; D015203:Reproducibility of Results; D012834:Silver / Q000652:urine*; D021241:Spectrometry, Mass, Electrospray Ionization; D013256:Steroids / Q000652:urine*
D045930:Anabolic Agents; D000728:Androgens; D013256:Steroids; D012834:Silver
LC-Ag+ CIS/MS/MS; anabolic androgenic steroids; doping control; ionization; silver coordination