Coating with a novel gentamicinpalmitate formulation prevents implant-associated osteomyelitis induced by methicillin-susceptible Staphylococcus aureus in a rat model.
OBJECTIVE
Implant-associated osteomyelitis still represents a demanding challenge due to unfavourable biological conditions, bacterial properties and incremental resistance to antibiotic treatment. Therefore different bactericide or bacteriostatic implant coatings have been developed recently to control local intramedullary infections. Controlled local release of gentamicin base from a highly lipophilic gentamicin palmitate compound achieves extended intramedullary retention times and thus may improve its bactericide effect.


METHODS
Forty male Sprague-Dawley rats were divided into two groups receiving an intramedullary femoral injection of 10(2) colony-forming units (CFU) of a common methicillin susceptible Staphylococcus aureus strain (MSSA Rosenbach) and either an uncoated femur nail (Group I) or a nail coated with gentamicin palmitate (Group II). Animals were observed for 28 and 42 days. Serum haptoglobin and relative weight gain were assessed as well as rollover cultures of explanted femur nails and histological scores of periprosthetic infection in dissected femurs.


RESULTS
Implants coated with gentamicin palmitate significantly reduced periprosthetic bacterial growth as well as signs of systemic inflammation compared with uncoated implants.


CONCLUSIONS
Gentamicin palmitate appears to be a viable coating for the prevention of implant-associated infections. These findings will have to be confirmed in larger animal models as well as in clinical trials.
Department of Orthopaedics and Rheumatology, University Hospital Marburg, Marburg, Germany.
Christian
C
Fölsch
Maike
M
Federmann
Klaus D
KD
Kuehn
Clemens
C
Kittinger
Stefan
S
Kogler
Gernot
G
Zarfel
Martina
M
Kerwat
Steve
S
Braun
Susanne
S
Fuchs-Winkelmann
Jürgen R J
JR
Paletta
Philip P
PP
Roessler
D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000627:therapeutic use; D019072:Antibiotic Prophylaxis / Q000379:methods; D020099:Coated Materials, Biocompatible / Q000008:administration & dosage; D004195:Disease Models, Animal; D005839:Gentamicins / Q000008:administration & dosage*; D008297:Male; D010019:Osteomyelitis / Q000382:microbiology / Q000517:prevention & control*; D016459:Prosthesis-Related Infections / Q000382:microbiology / Q000517:prevention & control*; D017207:Rats, Sprague-Dawley; D013203:Staphylococcal Infections / Q000517:prevention & control
D000900:Anti-Bacterial Agents; D020099:Coated Materials, Biocompatible; D005839:Gentamicins