Standing surface acoustic wave (SSAW)-based cell washing.
Cell/bead washing is an indispensable sample preparation procedure used in various cell studies and analytical processes. In this article, we report a standing surface acoustic wave (SSAW)-based microfluidic device for cell and bead washing in a continuous flow. In our approach, the acoustic radiation force generated in a SSAW field is utilized to actively extract cells or beads from their original medium. A unique configuration of tilted-angle standing surface acoustic wave (taSSAW) is employed in our device, enabling us to wash beads with >98% recovery rate and >97% washing efficiency. We also demonstrate the functionality of our device by preparing high-purity (>97%) white blood cells from lysed blood samples through cell washing. Our SSAW-based cell/bead washing device has the advantages of label-free manipulation, simplicity, high biocompatibility, high recovery rate, and high washing efficiency. It can be useful for many lab-on-a-chip applications.
Department of Engineering Science and Mechanics, The Pennsylvania State University, University Park, PA 16802, USA. junhuang@psu.edu.
Sixing
S
Li
Xiaoyun
X
Ding
Zhangming
Z
Mao
Yuchao
Y
Chen
Nitesh
N
Nama
Feng
F
Guo
Peng
P
Li
Lin
L
Wang
Craig E
CE
Cameron
Tony Jun
TJ
Huang
D003198:Computer Simulation; D003584:Cytological Techniques / Q000295:instrumentation* / Q000379:methods; D004867:Equipment Design; D006801:Humans; D007962:Leukocytes / Q000166:cytology; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods; D013016:Sound