Differentiation by random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) of Candida albicans isolated from upper respiratory tract in patients with non-small cell lung cancer.
Cancer patients are predisposed to fungal infections caused by Candida albicans, especially to oral or respiratory tract candidiasis. The aim of this study was to estimate genetic diversity by RAPD-PCR (random amplified polymorphic DNA-polymerase chain reaction) of C. albicans isolated from upper respiratory tract of 100 patients with non-small cell lung cancer. Among 52 strains, 34 genotypes were defined. 10 clusters comprising 28 (53.85%) isolates with similarity coefficient ≥ 80% were formed. The remaining 24 (46.15%) isolates represented individual genotypes. The RAPD-PCR technique revealed genomic variability within C. albicans isolated from upper respiratory tract of the cancer patients.
Department of Pharmaceutical Microbiology, Medical University of Lublin, Lublin, Poland.
Anna
A
Biernasiuk
Department of Pharmaceutical Microbiology, Medical University of Lublin, Lublin, Poland.
Izabela
I
Korona-Głowniak
Department of Pharmaceutical Microbiology, Medical University of Lublin, Lublin, Poland.
Agnieszka
A
Grzegorczyk
Department of Pharmaceutical Microbiology, Medical University of Lublin, Lublin, Poland.
Anna
A
Malm
D002176:Candida albicans / Q000235:genetics / Q000378:metabolism*; D002289:Carcinoma, Non-Small-Cell Lung / Q000382:microbiology*; D006801:Humans; D019105:Random Amplified Polymorphic DNA Technique / Q000379:methods*; D012137:Respiratory System / Q000382:microbiology*