Isolating cells from female/male blood mixtures using florescence in situ hybridization combined with low volume PCR and its application in forensic science.
To obtain single-source short tandem repeat (STR) profiles in trace female/male blood mixture samples, we combined florescence in situ hybridization (FISH), laser microdissection, and low volume PCR (LV-PCR) to isolate male/female cells and improve sensitivity. The results showed that isolation of as few as 10 leukocytes was sufficient to yield full STR profiles in fresh female or male blood samples for 32 independent tests with a low additional alleles rate (3.91%) and drop-out alleles rate (5.01%). Moreover, this procedure was tested in two fresh blood mixture series at three ratios (1:5, 1:10, and 1:20), two mock female/male blood mixture casework samples, and one practical casework sample. Male and female STR profiles were successfully detected in all of these samples, showing that this procedure could be used in forensic casework in the future.
Key Laboratory of Forensic Genetics, Ministry of Public Security, Institute of Forensic Science, Beijing, 100038, China.|Beijing Engineering Research Center of Crime Scene Evidence Examination, Institute of Forensic Science, Beijing, 100038, China.
Lei
L
Feng
Key Laboratory of Forensic Genetics, Ministry of Public Security, Institute of Forensic Science, Beijing, 100038, China. 13810666929@139.com.|Beijing Engineering Research Center of Crime Scene Evidence Examination, Institute of Forensic Science, Beijing, 100038, China. 13810666929@139.com.
Cai-Xia
CX
Li
Chinese Peoples Public Security University, Beijing, 100038, China.
Jun-Ping
JP
Han
Chinese Peoples Public Security University, Beijing, 100038, China.
Cheng
C
Xu
Key Laboratory of Forensic Genetics, Ministry of Public Security, Institute of Forensic Science, Beijing, 100038, China. 13911162826@139.com.|Beijing Engineering Research Center of Crime Scene Evidence Examination, Institute of Forensic Science, Beijing, 100038, China. 13911162826@139.com.
Lan
L
Hu
D001772:Blood Cell Count; D041321:Chromosomes, Human, X; D041322:Chromosomes, Human, Y; D016172:DNA Fingerprinting / Q000379:methods*; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D061346:Laser Capture Microdissection; D007962:Leukocytes / Q000166:cytology*; D008297:Male; D018895:Microsatellite Repeats; D016133:Polymerase Chain Reaction
DNA typing; Female/male blood mixture; Florescence in situ hybridization; Forensic science; Low volume PCR; Single-cell capture