Identification of histological patterns in clinically affected and unaffected palm regions in dupuytren's disease.
Dupuytren's disease is a fibro-proliferative disease characterized by a disorder of the extracellular matrix (ECM) and high myofibroblast proliferation. However, studies failed to determine if the whole palm fascia is affected by the disease. The objective of this study was to analyze several components of the extracellular matrix of three types of tissues-Dupuytren's diseased contracture cords (DDC), palmar fascia clinically unaffected by Dupuytren's disease contracture (NPF), and normal forehand fascia (NFF). Histological analysis, quantification of cells recultured from each type of tissue, mRNA microarrays and immunohistochemistry for smooth muscle actin (SMA), fibrillar ECM components and non-fibrillar ECM components were carried out. The results showed that DDC samples had abundant fibrosis with reticular fibers and few elastic fibers, high cell proliferation and myofibroblasts, laminin and glycoproteins, whereas NFF did not show any of these findings. Interestingly, NPF tissues had more cells showing myofibroblasts differentiation and more collagen and reticular fibers, laminin and glycoproteins than NFF, although at lower level than DDC, with similar elastic fibers than DDC. Immunohistochemical expression of decorin was high in DDC, whereas versican was highly expressed NFF, with no differences for aggrecan. Cluster analysis revealed that the global expression profile of NPF was very similar to DDC, and reculturing methods showed that cells corresponding to DDC tissues proliferated more actively than NPF, and NPF more actively than NFF. All these results suggest that NPF tissues may be affected, and that a modification of the therapeutic approach used for the treatment of Dupuytren's disease should be considered.
Department of Histology (Tissue Engineering Group), University of Granada, Granada, Spain, and Instituto de Investigación Biosanitaria ibs, Granada, Spain; Ph.D. Programme in Biomedicine, University of Granada, Granada, Spain.
Camilo-Andrés
CA
Alfonso-Rodríguez
Department of Histology (Tissue Engineering Group), University of Granada, Granada, Spain, and Instituto de Investigación Biosanitaria ibs, Granada, Spain.
Ingrid
I
Garzón
Division of Trauma and Orthopedic surgery, University Hospital San Cecilio, Granada, Spain.
Juan
J
Garrido-Gómez
Department of Histology (Tissue Engineering Group), University of Granada, Granada, Spain, and Instituto de Investigación Biosanitaria ibs, Granada, Spain.
Ana-Celeste-Ximenes
AC
Oliveira
Department of Histology (Tissue Engineering Group), University of Granada, Granada, Spain, and Instituto de Investigación Biosanitaria ibs, Granada, Spain.
Miguel-Ángel
MÁ
Martín-Piedra
Department of Histology (Tissue Engineering Group), University of Granada, Granada, Spain, and Instituto de Investigación Biosanitaria ibs, Granada, Spain.
Giuseppe
G
Scionti
Department of Histology (Tissue Engineering Group), University of Granada, Granada, Spain, and Instituto de Investigación Biosanitaria ibs, Granada, Spain.
Víctor
V
Carriel
Division of Trauma and Orthopedic surgery, University Hospital San Cecilio, Granada, Spain.
Pedro
P
Hernández-Cortés
Department of Histology (Tissue Engineering Group), University of Granada, Granada, Spain, and Instituto de Investigación Biosanitaria ibs, Granada, Spain.
Antonio
A
Campos
Department of Histology (Tissue Engineering Group), University of Granada, Granada, Spain, and Instituto de Investigación Biosanitaria ibs, Granada, Spain.
Miguel
M
Alaminos
D000199:Actins / Q000235:genetics / Q000378:metabolism; D000368:Aged; D002452:Cell Count; D049109:Cell Proliferation / Q000235:genetics; D002478:Cells, Cultured; D016000:Cluster Analysis; D003094:Collagen / Q000235:genetics / Q000378:metabolism; D004387:Dupuytren Contracture / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D005109:Extracellular Matrix / Q000378:metabolism / Q000473:pathology; D005205:Fascia / Q000378:metabolism / Q000473:pathology*; D020869:Gene Expression Profiling / Q000379:methods; D006023:Glycoproteins / Q000235:genetics / Q000378:metabolism; D006225:Hand / Q000473:pathology*; D006801:Humans; D007150:Immunohistochemistry; D007797:Laminin / Q000235:genetics / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D009130:Muscle, Smooth / Q000737:chemistry; D058628:Myofibroblasts / Q000378:metabolism / Q000473:pathology; D020411:Oligonucleotide Array Sequence Analysis; D060449:Wnt Signaling Pathway / Q000235:genetics
D000199:Actins; D006023:Glycoproteins; D007797:Laminin; D003094:Collagen