Use of Candida-specific chicken egg yolk antibodies to inhibit the adhering of Candida to denture base materials: prevention of denture stomatitis.
OBJECTIVE
Polyclonal anti-Candida chicken egg yolk antibodies (anti-IgY) were used to investigate the prevention of adherence of Candida species to denture base material in vitro.


BACKGROUND
Candida is a potential virulence factor that can cause systemic infection and even death in immunocompromised individuals. Because long-term antifungal treatment may lead to the emergence of drug-resistant strains, it is necessary to develop novel preventive measures and treatments for candidiasis.


METHODS
Three types of chicken egg yolk antibodies were used in this study: non-specific antibody (control IgY), Candida albicans-specific antibody (anti-C.a.IgY) and Candida glabrata-specific antibody (anti-C.g.IgY). A mixture of different dilutions of each antibody with a suspension of Candida species and denture base material was incubated for 3 h, and then the colony-forming units of Candida on the denture base material were counted.


RESULTS
Compared with control IgY, anti-C.a.IgY and anti-C.g.IgY significantly inhibited the adherence of C. albicans, but anti-C.a.IgY tended to be more potent than anti-C.g.IgY. The adherence of C. glabrata was also inhibited significantly by anti-C.a.IgY and anti-C.g.IgY with almost equivalent potency, indicating that their actions against C. glabrata were comparable.


CONCLUSIONS
This study revealed the inhibitory effects of anti-C.a.IgY and anti-C.g.IgY against the adherence of C. albicans and C. glabrata to denture base material. This finding indicates the possibility of a beneficial effect of IgYs for the prevention of denture stomatitis and candidiasis in clinical settings.
Department of Oral Surgery, Kagoshima University Medical and Dental Hospital, Kagoshima, Japan.
Yoshiaki
Y
Kamikawa
Department of Maxillofacial Diagnostic and Surgical Sciences, Field of Oral and Maxillofacial Rehabilitation, Kagoshima University Graduate School of Medical and Dental Sciences, Kagoshima, Japan.
Junichi
J
Fujisaki
Department of Oral Surgery, Kagoshima University Medical and Dental Hospital, Kagoshima, Japan.
Tomohiro
T
Nagayama
Department of Maxillofacial Diagnostic and Surgical Sciences, Field of Oral and Maxillofacial Rehabilitation, Kagoshima University Graduate School of Medical and Dental Sciences, Kagoshima, Japan.
Kiyotsugu
K
Kawasaki
Department of Maxillofacial Diagnostic and Surgical Sciences, Field of Oral and Maxillofacial Rehabilitation, Kagoshima University Graduate School of Medical and Dental Sciences, Kagoshima, Japan.
Daisuke
D
Hirabayashi
Department of Maxillofacial Diagnostic and Surgical Sciences, Field of Oral and Maxillofacial Rehabilitation, Kagoshima University Graduate School of Medical and Dental Sciences, Kagoshima, Japan.
Tomofumi
T
Hamada
Department of Maxillofacial Diagnostic and Surgical Sciences, Field of Oral and Maxillofacial Rehabilitation, Kagoshima University Graduate School of Medical and Dental Sciences, Kagoshima, Japan.
Ryoich
R
Sakamoto
Department of Oral Surgery, Kagoshima University Medical and Dental Hospital, Kagoshima, Japan.|Department of Maxillofacial Diagnostic and Surgical Sciences, Field of Oral and Maxillofacial Rehabilitation, Kagoshima University Graduate School of Medical and Dental Sciences, Kagoshima, Japan.
Hiroshi
H
Mukai
Department of Oral Surgery, Kagoshima University Medical and Dental Hospital, Kagoshima, Japan.|Department of Maxillofacial Diagnostic and Surgical Sciences, Field of Oral and Maxillofacial Rehabilitation, Kagoshima University Graduate School of Medical and Dental Sciences, Kagoshima, Japan.
Kazumasa
http://orcid.org/0000-0003-4797-100X
K
Sugihara
D000818:Animals; D000906:Antibodies / Q000378:metabolism*; D002175:Candida / Q000378:metabolism*; D002177:Candidiasis / Q000517:prevention & control; D002645:Chickens; D003819:Denture Bases / Q000382:microbiology*; D004530:Egg Yolk; D006801:Humans; D013282:Stomatitis, Denture / Q000517:prevention & control*
D000906:Antibodies
Candida; egg yolk globulin; oral candidiasis; prophylaxis