Green and sensitive supercritical fluid chromatographic-tandem mass spectrometric method for the separation and determination of flutriafol enantiomers in vegetables, fruits, and soil.
A green and sensitive chiral analytical method was developed to determine flutriafol enantiomers in vegetables (tomato, cucumber), fruits (apple, grape), and soil by supercritical fluid chromatography-tandem mass spectrometry. The enantioseparation was performed within 3.50 min using Chiralpak IA-3 column with CO2/methanol (88:12, v/v) as the mobile phase at a 2.2 mL/min flow rate. The postcolumn compensation technology provided with 1% formic acid/methanol greatly improved the ionization efficiency of mass spectrometry. Column temperature, auto back pressure regulator pressure, and flow rate of compensation solvent were optimized to 30 °C, 2200 psi, and 0.1 mL/min, respectively. The simple and fast QuEChERS pretreatment method was adopted. Mean recoveries for flutriafol enantiomers were 77.2-98.9% with RSDs ≤ 9.6% in all matrices. The limits of quantification ranged from 0.41 to 1.18 μg/kg. Well-applied to analyze authentic samples, the developed method could act as a versatile strategy for the analysis of flutriafol enantiomers in food and environmental matrices.
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences , Beijing 100193, People's Republic of China.
Yan
Y
Tao
Fengshou
F
Dong
Jun
J
Xu
Xingang
X
Liu
Youpu
Y
Cheng
Na
N
Liu
Zenglong
Z
Chen
Yongquan
Y
Zheng
D025924:Chromatography, Supercritical Fluid / Q000379:methods*; D005506:Food Contamination / Q000032:analysis; D005638:Fruit / Q000737:chemistry*; D055772:Green Chemistry Technology; D057230:Limit of Detection; D015203:Reproducibility of Results; D012987:Soil / Q000737:chemistry*; D012989:Soil Pollutants / Q000032:analysis; D013237:Stereoisomerism; D053719:Tandem Mass Spectrometry / Q000379:methods*; D014230:Triazoles / Q000032:analysis*; D014675:Vegetables / Q000737:chemistry*
D012987:Soil; D012989:Soil Pollutants; D014230:Triazoles; C543009:flutriafol
QuEChERS; SFC-MS/MS; chiral; flutriafol; green