Fluorescence correlation spectroscopy.
Fluorescence fluctuation spectroscopy techniques allow the quantification of fluorescent molecules present at the nanomolar concentration level. After a brief introduction to the technique, this chapter presents a protocol including background information in order to measure and quantify the molecular interaction of two signaling proteins inside the living cell using fluorescence cross-correlation spectroscopy.
Department Molecular Cytology, van Leeuwenhoek Centre for Advanced Microscopy (LCAM), University of Amsterdam, Sciencepark 904, 1098 XH, Amsterdam, The Netherlands, m.a.hink@uva.nl.
Mark A
MA
Hink
D002138:Calibration; D005456:Fluorescent Dyes; D006367:HeLa Cells; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000378:metabolism*; D025941:Protein Interaction Mapping / Q000379:methods*; D013050:Spectrometry, Fluorescence / Q000295:instrumentation* / Q000379:methods*; D014162:Transfection
D005456:Fluorescent Dyes; D047908:Intracellular Signaling Peptides and Proteins