Multifunctional spider silk polymers for gene delivery to human mesenchymal stem cells.
Non-viral gene delivery systems are important transport vehicles that can be safe and effective alternatives to currently available viral systems. A new family of multifunctional spider silk-based gene carriers was bioengineered and found capable of targeting human mesenchymal stem cells (hMSCs). These carriers successfully delivered DNA to the nucleus of these mammalian cells. The presence of specific functional sequences in the recombinant proteins, such as a nuclear localization sequence (NLS) of the large tumor (T) antigen of the Simian virus 40 (SV40 ), an hMSC high affinity binding peptide (HAB), and a translocation motif (TLM) of the hepatitis-B virus surface protein (PreS2), and their roles in mitigation and enhancement of gene transfection efficiency towards hMSCs were characterized. The results demonstrate that these bioengineered spider silk proteins serve as effective carriers, without the well-known complications associated with viral delivery systems.
Department of Biomedical Engineering, Tufts University, Medford, Massachusetts, 02155.
Olena S
OS
Tokareva
Department of Biomedical Engineering, Tufts University, Medford, Massachusetts, 02155.
Dean L
DL
Glettig
Department of Biomedical Engineering, Tufts University, Medford, Massachusetts, 02155.
Rosalyn D
RD
Abbott
Department of Biomedical Engineering, Tufts University, Medford, Massachusetts, 02155.
David L
DL
Kaplan
D000818:Animals; D018014:Gene Transfer Techniques; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism*; D047011:Silk / Q000737:chemistry*; D013112:Spiders
D047011:Silk
human mesenchymal stem cells; non-viral gene delivery; nuclear targeting; recombinant DNA technology; spider silks