Metabolomics reveals significant impairments in the immune system of the APP/PS1 transgenic mice of Alzheimer's disease.
Inflammatory processes and other failures related to the immune system are common features associated with Alzheimer's disease (AD), in both brain and the peripheral system. Thus, the study of the main organs of the immune system may have a great potential for the elucidation of pathological mechanisms underlying these abnormalities. This is the first metabolomic investigation performed in spleen and thymus from transgenic mice of AD. Tissues were fingerprinted using a metabolomic platform comprising GC-MS and ultra-HPLC-MS. Multivariate statistics demonstrated significant differences in numerous metabolites between the APP/PS1 mice and wild-type controls, and it was proven that multiple biochemical pathways are disturbed in these organs including abnormal metabolism of phospholipids, energy deficiencies, altered homeostasis of amino acids, oxidative stress, and others. Therefore, these findings highlight the importance of the proper metabolic functioning of peripheral immune system in the development of neurodegenerative disorders such as AD.
Department of Chemistry and CCMM, Faculty of Experimental Sciences, University of Huelva, Campus de El Carmen, Huelva, Spain; Campus of Excellence International ceiA3, University of Huelva, Huelva, Spain; Research Center of Health and Environment (CYSMA), University of Huelva, Campus de El Carmen, Huelva, Spain.
Raúl
R
González-Domínguez
Tamara
T
García-Barrera
Javier
J
Vitorica
José Luis
JL
Gómez-Ariza
D000544:Alzheimer Disease / Q000276:immunology / Q000378:metabolism*; D016564:Amyloid beta-Protein Precursor / Q000235:genetics / Q000378:metabolism; D000818:Animals; D016022:Case-Control Studies; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004195:Disease Models, Animal; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry; D007107:Immune System / Q000378:metabolism*; D008297:Male; D055432:Metabolomics / Q000379:methods*; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D013154:Spleen / Q000276:immunology / Q000378:metabolism; D013950:Thymus Gland / Q000276:immunology / Q000378:metabolism
D016564:Amyloid beta-Protein Precursor
APP/PS1; Alzheimer's disease; Metabolomics; Spleen; Thymus