Association of BRAFV600E mutation with clinicopathological features of papillary thyroid carcinoma: a study on a Chinese population.
BACKGROUND
The new finding of the heterogeneous distribution of BRAF(V600E) mutation in primary papillary thyroid carcinoma suggested the percentage of BRAF(V600E) alleles should be taken into consideration when evaluating its association with clinicopathological features of papillary thyroid carcinoma. The aim of this study was to detect both the presence and the percentage of BRAF(V600E) alleles in fine-needle aspiration biopsy samples and to assess its association with clinicopathological characteristics of papillary thyroid carcinoma in a Chinese population.


METHODS
Fine needle aspiration samples were collected in a total of 182 patients (132 conventional papillary thyroid carcinomas and 50 goiters). The associations of the presence and percentage of BRAF(V600E) alleles genotyped by pyrosequencing with clinicopathological characteristics were evaluated in papillary thyroid carcinomas.


RESULTS
80 (60.61%) of papillary thyroid carcinomas exhibited BRAF(V600E) mutation in a range of 7.7% to 46.3% of the total BRAF alleles. The presence of BRAF(V600E) mutation was significantly associated with extrathyroidal invasion. There was no significant difference between the presence of BRAF(V600E) mutation and other clinicopathological features. It was not found that the significant relationship between percentage of BRAF(V600E) alleles and clinicopathological characteristics.


CONCLUSIONS
We concluded that the presence of BRAF(V600E) could be preoperatively predictive of extrathyroidal invasion in a Chinese population.
Department of Endocrinology, The First Affiliated Hospital of Xi'an Jiaotong University Health Science Center Xi'an 710061, P. R. China.
Shu
S
Liu
Department of Endocrinology, The First Affiliated Hospital of Xi'an Jiaotong University Health Science Center Xi'an 710061, P. R. China.
Bingfei
B
Zhang
Department of Endocrinology, The First Affiliated Hospital of Xi'an Jiaotong University Health Science Center Xi'an 710061, P. R. China.
Yanru
Y
Zhao
Department of Endocrinology, The First Affiliated Hospital of Xi'an Jiaotong University Health Science Center Xi'an 710061, P. R. China.
Pu
P
Chen
Department of Endocrinology, The First Affiliated Hospital of Xi'an Jiaotong University Health Science Center Xi'an 710061, P. R. China.
Meiju
M
Ji
Department of Endocrinology, The First Affiliated Hospital of Xi'an Jiaotong University Health Science Center Xi'an 710061, P. R. China.
Peng
P
Hou
Department of Endocrinology, The First Affiliated Hospital of Xi'an Jiaotong University Health Science Center Xi'an 710061, P. R. China.
Bingyin
B
Shi
D000328:Adult; D044466:Asian People / Q000235:genetics; D044963:Biopsy, Fine-Needle; D002277:Carcinoma / Q000201:enzymology / Q000208:ethnology / Q000235:genetics* / Q000473:pathology / Q000601:surgery; D002291:Carcinoma, Papillary; D002681:China / Q000453:epidemiology; D004252:DNA Mutational Analysis; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D006801:Humans; D008297:Male; D008875:Middle Aged; D009154:Mutation; D009361:Neoplasm Invasiveness; D010641:Phenotype; D011237:Predictive Value of Tests; D011379:Prognosis; D048493:Proto-Oncogene Proteins B-raf / Q000235:genetics*; D000077273:Thyroid Cancer, Papillary; D013964:Thyroid Neoplasms / Q000201:enzymology / Q000208:ethnology / Q000235:genetics* / Q000473:pathology / Q000601:surgery
C482119:BRAF protein, human; D048493:Proto-Oncogene Proteins B-raf
Papillary thyroid carcinoma (PTC); percentage of BRAFV600E alleles; preoperative risk stratification; pyrosequencing