Alteration in the intrafollicular thiol-redox system in infertile women with endometriosis.
The aim of this study was to compare intrafollicular biomarkers of thiol-redox system and chronic inflammation in infertile patients with and without endometriosis, and examine correlations between biomarkers and IVF outcomes. The study included 65 patients receiving IVF: 31 patients with endometriosis vs 34 patients without endometriosis. Follicular fluid (FF) was obtained from a single-dominant follicle during oocyte retrieval and stored at -70 °C. Malondialdehyde, superoxide dismutase, glutathione (GSH), glutathione peroxidase 3 (GPX3), thioredoxin (TRX), TRX-binding protein 2 (TBP2), and peroxiredoxin-4 levels were measured in the FF samples by ELISAs as biomarkers of oxidative stress. The inflammatory cytokines interleukin 1 beta (IL1β), IL6, IL8, and tumor-necrosis factor alpha (TNFα) were also measured by ELISAs. GSH levels were significantly lower in the endometriosis group compared with the controls. TBP2 levels were significantly higher in the endometriosis group. IL6, IL8, and TNFα levels were significantly higher in the endometriosis group. The levels of all of the inflammatory cytokines positively correlated with the levels of TRX. GSH levels positively correlated with the number of high-quality embryos. GPX3 and TRX levels negatively correlated with the percentage of mature oocytes. TNFα levels negatively correlated with the cumulative embryo score per embryo. Logistic regression analysis revealed that the number of high-quality embryos was an independent factor predicting clinical pregnancy. In conclusion, there may be an imbalance in the thiol-redox system and increased levels of inflammatory cytokines in the intrafollicular microenvironment of infertile patients with endometriosis, which may affect the qualities of the oocyte and embryo.
Department of Obstetrics and GynecologySeverance Hospital, Yonsei University College of Medicine, Seoul 120-752, Republic of KoreaInstitute of Women's Life Medical ScienceYonsei University College of Medicine, Seoul 120-752, Republic of KoreaDepartment of Obstetrics and GynecologyGangnam Severance Hospital, Yonsei University College of Medicine, 146-92 Dogok-dong, Kangnam-gu, Seoul 135-720, Republic of KoreaDepartment of Obstetrics and GynecologyCollege of Medicine, Seoul National University, 28 Yeongeon-Dong, Jongno-Gu, Seoul 110-744, Republic of Korea Department of Obstetrics and GynecologySeverance Hospital, Yonsei University College of Medicine, Seoul 120-752, Republic of KoreaInstitute of Women's Life Medical ScienceYonsei University College of Medicine, Seoul 120-752, Republic of KoreaDepartment of Obstetrics and GynecologyGangnam Severance Hospital, Yonsei University College of Medicine, 146-92 Dogok-dong, Kangnam-gu, Seoul 135-720, Republic of KoreaDepartment of Obstetrics and GynecologyCollege of Medicine, Seoul National University, 28 Yeongeon-Dong, Jongno-Gu, Seoul 110-744, Republic of Korea.
Young Sik
YS
Choi
Department of Obstetrics and GynecologySeverance Hospital, Yonsei University College of Medicine, Seoul 120-752, Republic of KoreaInstitute of Women's Life Medical ScienceYonsei University College of Medicine, Seoul 120-752, Republic of KoreaDepartment of Obstetrics and GynecologyGangnam Severance Hospital, Yonsei University College of Medicine, 146-92 Dogok-dong, Kangnam-gu, Seoul 135-720, Republic of KoreaDepartment of Obstetrics and GynecologyCollege of Medicine, Seoul National University, 28 Yeongeon-Dong, Jongno-Gu, Seoul 110-744, Republic of Korea Department of Obstetrics and GynecologySeverance Hospital, Yonsei University College of Medicine, Seoul 120-752, Republic of KoreaInstitute of Women's Life Medical ScienceYonsei University College of Medicine, Seoul 120-752, Republic of KoreaDepartment of Obstetrics and GynecologyGangnam Severance Hospital, Yonsei University College of Medicine, 146-92 Dogok-dong, Kangnam-gu, Seoul 135-720, Republic of KoreaDepartment of Obstetrics and GynecologyCollege of Medicine, Seoul National University, 28 Yeongeon-Dong, Jongno-Gu, Seoul 110-744, Republic of Korea.
SiHyun
S
Cho
Department of Obstetrics and GynecologySeverance Hospital, Yonsei University College of Medicine, Seoul 120-752, Republic of KoreaInstitute of Women's Life Medical ScienceYonsei University College of Medicine, Seoul 120-752, Republic of KoreaDepartment of Obstetrics and GynecologyGangnam Severance Hospital, Yonsei University College of Medicine, 146-92 Dogok-dong, Kangnam-gu, Seoul 135-720, Republic of KoreaDepartment of Obstetrics and GynecologyCollege of Medicine, Seoul National University, 28 Yeongeon-Dong, Jongno-Gu, Seoul 110-744, Republic of Korea Department of Obstetrics and GynecologySeverance Hospital, Yonsei University College of Medicine, Seoul 120-752, Republic of KoreaInstitute of Women's Life Medical ScienceYonsei University College of Medicine, Seoul 120-752, Republic of KoreaDepartment of Obstetrics and GynecologyGangnam Severance Hospital, Yonsei University College of Medicine, 146-92 Dogok-dong, Kangnam-gu, Seoul 135-720, Republic of KoreaDepartment of Obstetrics and GynecologyCollege of Medicine, Seoul National University, 28 Yeongeon-Dong, Jongno-Gu, Seoul 110-744, Republic of Korea.
Seok Kyo
SK
Seo
Department of Obstetrics and GynecologySeverance Hospital, Yonsei University College of Medicine, Seoul 120-752, Republic of KoreaInstitute of Women's Life Medical ScienceYonsei University College of Medicine, Seoul 120-752, Republic of KoreaDepartment of Obstetrics and GynecologyGangnam Severance Hospital, Yonsei University College of Medicine, 146-92 Dogok-dong, Kangnam-gu, Seoul 135-720, Republic of KoreaDepartment of Obstetrics and GynecologyCollege of Medicine, Seoul National University, 28 Yeongeon-Dong, Jongno-Gu, Seoul 110-744, Republic of Korea Department of Obstetrics and GynecologySeverance Hospital, Yonsei University College of Medicine, Seoul 120-752, Republic of KoreaInstitute of Women's Life Medical ScienceYonsei University College of Medicine, Seoul 120-752, Republic of KoreaDepartment of Obstetrics and GynecologyGangnam Severance Hospital, Yonsei University College of Medicine, 146-92 Dogok-dong, Kangnam-gu, Seoul 135-720, Republic of KoreaDepartment of Obstetrics and GynecologyCollege of Medicine, Seoul National University, 28 Yeongeon-Dong, Jongno-Gu, Seoul 110-744, Republic of Korea.
Joo Hyun
JH
Park
Department of Obstetrics and GynecologySeverance Hospital, Yonsei University College of Medicine, Seoul 120-752, Republic of KoreaInstitute of Women's Life Medical ScienceYonsei University College of Medicine, Seoul 120-752, Republic of KoreaDepartment of Obstetrics and GynecologyGangnam Severance Hospital, Yonsei University College of Medicine, 146-92 Dogok-dong, Kangnam-gu, Seoul 135-720, Republic of KoreaDepartment of Obstetrics and GynecologyCollege of Medicine, Seoul National University, 28 Yeongeon-Dong, Jongno-Gu, Seoul 110-744, Republic of Korea dr222@yuhs.ac seokhyun@snu.ac.kr.
Seok Hyun
SH
Kim
Department of Obstetrics and GynecologySeverance Hospital, Yonsei University College of Medicine, Seoul 120-752, Republic of KoreaInstitute of Women's Life Medical ScienceYonsei University College of Medicine, Seoul 120-752, Republic of KoreaDepartment of Obstetrics and GynecologyGangnam Severance Hospital, Yonsei University College of Medicine, 146-92 Dogok-dong, Kangnam-gu, Seoul 135-720, Republic of KoreaDepartment of Obstetrics and GynecologyCollege of Medicine, Seoul National University, 28 Yeongeon-Dong, Jongno-Gu, Seoul 110-744, Republic of Korea Department of Obstetrics and GynecologySeverance Hospital, Yonsei University College of Medicine, Seoul 120-752, Republic of KoreaInstitute of Women's Life Medical ScienceYonsei University College of Medicine, Seoul 120-752, Republic of KoreaDepartment of Obstetrics and GynecologyGangnam Severance Hospital, Yonsei University College of Medicine, 146-92 Dogok-dong, Kangnam-gu, Seoul 135-720, Republic of KoreaDepartment of Obstetrics and GynecologyCollege of Medicine, Seoul National University, 28 Yeongeon-Dong, Jongno-Gu, Seoul 110-744, Republic of Korea dr222@yuhs.ac seokhyun@snu.ac.kr.
Byung Seok
BS
Lee
D000328:Adult; D015415:Biomarkers / Q000032:analysis; D016207:Cytokines / Q000032:analysis; D004715:Endometriosis / Q000378:metabolism* / Q000601:surgery; D005260:Female; D005307:Fertilization in Vitro; D015571:Follicular Fluid / Q000737:chemistry; D006801:Humans; D007247:Infertility, Female / Q000378:metabolism*; D007249:Inflammation / Q000378:metabolism; D006080:Ovarian Follicle / Q000378:metabolism*; D010062:Ovulation Induction; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D011247:Pregnancy; D011446:Prospective Studies; D013438:Sulfhydryl Compounds / Q000378:metabolism*
D015415:Biomarkers; D016207:Cytokines; D013438:Sulfhydryl Compounds