Enzymatic synthesis of a DNA triblock copolymer that is composed of natural and unnatural nucleotides.
DNA molecules have come under the spotlight as potential templates for the fabrication of nanoscale products, such as molecular-scale electronic or photonic devices. Herein, we report an enhanced approach for the synthesis of oligoblock copolymer-type DNA by using the Klenow fragment exonuclease minus of E. coli DNA polymerase I (KF(-) ) in a multi-step reaction with natural and unnatural nucleotides. First, we confirmed the applicability of unnatural nucleotides with 7-deaza-nucleosides-which was expected because they were non-metalized nucleotides-on the unique polymerization process known as the "strand-slippage model". Because the length of the DNA sequence could be controlled by tuning the reaction time, analogous to a living polymerization reaction on this process, stepwise polymerization provided DNA block copolymers with natural and unnatural bases. AFM images showed that this DNA block copolymer could be metalized sequence-selectively. This approach could expand the utility of DNA as a template.
Research Institute for Electronic Science, Hokkaido University, Sapporo, Hokkaido, 001-0021 (Japan).
Hideyuki
H
Mitomo
Yukie
Y
Watanabe
Yasutaka
Y
Matsuo
Kenichi
K
Niikura
Kuniharu
K
Ijiro
D002942:Circular Dichroism; D004247:DNA / Q000138:chemical synthesis / Q000737:chemistry*; D004256:DNA Polymerase I / Q000378:metabolism; D004926:Escherichia coli / Q000201:enzymology; D018625:Microscopy, Atomic Force; D010984:Platinum / Q000737:chemistry; D011089:Polydeoxyribonucleotides / Q000138:chemical synthesis / Q000737:chemistry; D058105:Polymerization; D044366:Transition Temperature
D011089:Polydeoxyribonucleotides; C016681:poly(dG).poly(dC); D010984:Platinum; D004247:DNA; D004256:DNA Polymerase I
DNA; enzymes; nucleotides; polymerization; template synthesis