Identification of RNA sequence isomer by isotope labeling and LC-MS/MS.
Recently, we developed a method for modified ribonucleic acid (RNA) analysis based on the comparative analysis of RNA digests (CARD). Within this CARD approach, sequence or modification differences between two samples are identified through differential isotopic labeling of two samples. Components present in both samples will each be labeled, yielding doublets in the CARD mass spectrum. Components unique to only one sample should be detected as singlets. A limitation of the prior singlet identification strategy occurs when the two samples contain components of unique sequence but identical base composition. At the first stage of mass spectrometry, these sequence isomers cannot be differentiated and would appear as doublets rather than singlets. However, underlying sequence differences should be detectable by collision-induced dissociation tandem mass spectrometry (CID MS/MS), as y-type product ions will retain the original enzymatically incorporated isotope label. Here, we determine appropriate instrumental conditions that enable CID MS/MS of isotopically labeled ribonuclease T1 (RNase T1) digestion products such that the original isotope label is maintained in the product ion mass spectrum. Next, we demonstrate how y-type product ions can be used to differentiate singlets and doublets from isomer sequences. We were then able to extend the utility of this approach by using CID MS/MS for the confirmation of an expected RNase T1 digestion product within the CARD analysis of an Escherichia coli mutant strain even in the presence of interfering and overlapping digestion products from other transfer RNAs.
Rieveschl Laboratories for Mass Spectrometry, Department of Chemistry, University of Cincinnati, PO Box 210172, Cincinnati, OH, 45221-0172, USA.
Siwei
S
Li
Patrick A
PA
Limbach
D001483:Base Sequence; D002853:Chromatography, Liquid / Q000379:methods; D004926:Escherichia coli / Q000235:genetics; D007536:Isomerism; D007553:Isotope Labeling / Q000379:methods; D008969:Molecular Sequence Data; D012313:RNA / Q000737:chemistry* / Q000378:metabolism; D012260:Ribonucleases / Q000378:metabolism; D017423:Sequence Analysis, RNA / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods
D012313:RNA; D012260:Ribonucleases
RNA isomers; collision-induced dissociation; comparative analysis; isotopic labeling; liquid chromatography-tandem mass spectrometry; ncRNA; tRNA