Genotoxicity and cytotoxicity of sevoflurane in two human cell lines in vitro with ionizing radiation.
OBJECTIVE
To determine the in vitro toxicity of different concentrations of sevoflurane in cells exposed to X-ray.


METHODS
The genotoxic effects of sevofluorane were studied by means of the micronucleus test in cytokinesis-blocked cells of irradiated human lymphocytes. Subsequently, its cytotoxic effects on PNT2 (normal prostate) cells was determined using the cell viability test (MTT) and compared with those induced by different doses of X-rays.


RESULTS
A dose- and time-dependent cytotoxic effect of sevofluorane on PNT2 cells was determined (p >0.001) and a dose-dependent genotoxic effect of sevofluorane was established (p >0.001). However, at volumes lower than 30 μL of sevofluorane at 100%, a non-toxic effect on PNT2 cells was shown.


CONCLUSIONS
Sevofluorane demonstrates a genotoxic capacity as determined in vitro by micronucleus test in cytokinesis-blocked cells of irradiated human lymphocytes.
Radiology and Physical Medicine Department. Faculty of Medicine/Dentistry. University of Murcia. 30100 Espinardo. Murcia. Spain.
Miguel
M
Alcaraz
Radiology and Physical Medicine Department. Faculty of Medicine/Dentistry. University of Murcia. 30100 Espinardo. Murcia. Spain.
Samuel
S
Quesada
Nursing Department. Faculty of Nursing. University of Murcia. 30100 Espinardo. Murcia. Spain.
David
D
Armero
Anesthesiology Department. Virgen de la Arrixaca University Hospital, 30120. El Palmar. Murcia. Spain.
Rocio
R
Martin-Gíl
Radiology and Physical Medicine Department. Faculty of Medicine/Dentistry. University of Murcia. 30100 Espinardo. Murcia. Spain.
Amparo
A
Olivares
Applied Radiation Biology Centre, Radiological and Medical Sciences Research Institute, Ghana Atomic Energy Commission, Legon-Accra, Ghana.
G Daniel
GD
Achel
D018685:Anesthetics, Inhalation / Q000008:administration & dosage / Q000633:toxicity*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005260:Female; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects* / Q000378:metabolism / Q000528:radiation effects; D008297:Male; D008738:Methyl Ethers / Q000008:administration & dosage / Q000633:toxicity*; D015162:Micronucleus Tests; D009153:Mutagens / Q000008:administration & dosage / Q000633:toxicity; D011467:Prostate / Q000166:cytology / Q000187:drug effects*; D011839:Radiation, Ionizing; D000077149:Sevoflurane; D013997:Time Factors
D018685:Anesthetics, Inhalation; D008738:Methyl Ethers; D009153:Mutagens; D000077149:Sevoflurane
Anaesthesia; genotoxicity; micronucleus; radiation effects; sevofluorane