Lactobacillus acidophilus L-92 Cells Activate Expression of Immunomodulatory Genes in THP-1 Cells.
To understand the immunomodulatory effects of Lactobacillus acidophilus L-92 cells suggested from our previous study of in vivo anti-allergy and anti-virus effects, host immune responses in macrophage-like THP-1 cells after 4 h (the early phase) and 24 h (the late phase) of cocultivation with L-92 cells were investigated by transcriptome analysis. In the early phase of L-92 treatment, various transcription regulator genes, such as, NFkB1, NFkB2, JUN, HIVEP2 and RELB, and genes encoding chemokines and cytokines, such as CCL4, CXCL11, CCL3 and TNF, were upregulated. Two transmembrane receptor genes, TLR7 and ICAM1, were also upregulated in the early phase of treatment. In contrast, many transmembrane receptor genes, such as IL7R, CD80, CRLF2, CD86, CD5, HLA-DQA1, IL2RA, IL15RA and CSF2RA, and some cytokine genes, including IL6, IL23A and CCL22, were significantly upregulated in the late phase after L-92 exposure. Some genes encoding cytokines, such as IL1A, IL1B and IL8, and the enzyme IDO1 were upregulated at both the early and the late phases of treatment. These results suggest that probiotic L-92 might promote Th1 and regulatory T-cell responses by activation of the MAPK signaling pathway, followed by the NOD-like receptor signaling pathway in THP-1 cells.
Microbiology and Fermentation Laboratory, Calpis Co. Ltd., 5-11-10 Fuchinobe, Chuo-ku, Sagamihara-shi, Kanagawa 252-0206, Japan.
Sae
S
Yanagihara
Microbiology and Fermentation Laboratory, Calpis Co. Ltd., 5-11-10 Fuchinobe, Chuo-ku, Sagamihara-shi, Kanagawa 252-0206, Japan.
Hiroaki
H
Goto
Microbiology and Fermentation Laboratory, Calpis Co. Ltd., 5-11-10 Fuchinobe, Chuo-ku, Sagamihara-shi, Kanagawa 252-0206, Japan.
Tatsuhiko
T
Hirota
Intestinal Microbe Symbiosis Laboratory, RIKEN, Wako, Saitama 351-0198, Japan ; Laboratory for Intestinal Ecosystem, RCAI, RIKEN Center for Integrative Medical Sciences, Yokohama, Kanagawa 230-0045, Japan ; Immunobiology Laboratory, Graduate School of Medical Life Science, Yokohama City University, Yokohama, Kanagawa 230-0045, Japan ; Institute for Advanced Biosciences, Keio University, Tsuruoka, Yamagata 997-0052, Japan.
Shinji
S
Fukuda
Intestinal Microbe Symbiosis Laboratory, RIKEN, Wako, Saitama 351-0198, Japan ; Laboratory for Intestinal Ecosystem, RCAI, RIKEN Center for Integrative Medical Sciences, Yokohama, Kanagawa 230-0045, Japan ; Immunobiology Laboratory, Graduate School of Medical Life Science, Yokohama City University, Yokohama, Kanagawa 230-0045, Japan.
Hiroshi
H
Ohno
Intestinal Microbe Symbiosis Laboratory, RIKEN, Wako, Saitama 351-0198, Japan ; Research and Development Planning Department, Calpis Co. Ltd., 5-11-10 Fuchinobe, Chuo-ku, Sagamihara-shi, Kanagawa 252-0206, Japan.
Naoyuki
N
Yamamoto
Lactobacillus acidophilus L-92; THP-1; cytokine; transcription regulator; transcriptome analysis