Deregulation of serum microRNA expression is associated with cigarette smoking and lung cancer.
Lung cancer is the leading cause of cancer-related death and cigarette smoking is the main risk factor for lung cancer. Circulating microRNAs (miRNAs) are considered potential biomarkers of various cancers, including lung cancer. However, it is unclear whether changes in circulating miRNAs are associated with smoking and smoking-related lung cancer. In this study, we determined the serum miRNA profiles of 10 nonsmokers, 10 smokers, and 10 lung-cancer patients with miRCURY LNA microRNA arrays. The differentially expressed miRNAs were then confirmed in a larger sample. We found that let-7i-3p and miR-154-5p were significantly downregulated in the sera of smokers and lung-cancer patients, so the serum levels of let-7i-3p and miR-154-5p are associated with smoking and smoking-related lung cancer. The areas under receiver operating characteristic curves for let-7i-3p and miR-154-5p were approximately 0.892 and 0.957, respectively. In conclusion, our results indicate that changes in serum miRNAs are associated with cigarette smoking and lung cancer and that let-7i-3p and miR-154-5p are potential biomarkers of smoking-related lung cancer.
State Key Laboratory of Respiratory Diseases, Institute for Chemical Carcinogenesis, Guangzhou Medical University, 195 Dongfeng Road West, Guangzhou 510182, China ; The First Affiliated Hospital of Guangzhou Medical University, Guangzhou 510120, China.
Jinkun
J
Huang
State Key Laboratory of Respiratory Diseases, Institute for Chemical Carcinogenesis, Guangzhou Medical University, 195 Dongfeng Road West, Guangzhou 510182, China.
Jianjun
J
Wu
State Key Laboratory of Respiratory Diseases, Institute for Chemical Carcinogenesis, Guangzhou Medical University, 195 Dongfeng Road West, Guangzhou 510182, China.
Yuanqi
Y
Li
State Key Laboratory of Respiratory Diseases, Institute for Chemical Carcinogenesis, Guangzhou Medical University, 195 Dongfeng Road West, Guangzhou 510182, China.
Xun
X
Li
State Key Laboratory of Respiratory Diseases, Institute for Chemical Carcinogenesis, Guangzhou Medical University, 195 Dongfeng Road West, Guangzhou 510182, China.
Ti
T
Yang
State Key Laboratory of Respiratory Diseases, Institute for Chemical Carcinogenesis, Guangzhou Medical University, 195 Dongfeng Road West, Guangzhou 510182, China.
Qiaoyuan
Q
Yang
State Key Laboratory of Respiratory Diseases, Institute for Chemical Carcinogenesis, Guangzhou Medical University, 195 Dongfeng Road West, Guangzhou 510182, China.
Yiguo
Y
Jiang
D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000097:blood; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008175:Lung Neoplasms / Q000097:blood / Q000235:genetics*; D008297:Male; D035683:MicroRNAs / Q000096:biosynthesis* / Q000097:blood; D008875:Middle Aged; D011379:Prognosis; D012907:Smoking / Q000009:adverse effects / Q000097:blood / Q000235:genetics*
D014408:Biomarkers, Tumor; C561122:MIRN154 microRNA, human; D035683:MicroRNAs; C487931:mirnlet7 microRNA, human