[Adrenomedullin inhibits TGF-β1-induced procollagen expression in cultured human fetal lung fibroblasts via Smad2/3 pathway].
OBJECTIVE
To observe the effects of adrenomedullin (AM) on transforming growth factor- β1 (TGF-β1) induced the expression of procollagen type 1 alpha 1 (Col1α1) and procollagen type 3 alpha 1 (Col3α1) as well as Smad2/3 phosphorylation in human fetal lung fibroblasts (HFLFs).


METHODS
Primary HFLFs were cultured in vitro. After treated with TGF-β1 and/or AM, the expression levels of Col1α1 and Col3α1 mRNA were determined by reverse transcription PCR (RT-PCR), and phospho-Smad2/3 (p-Smad2/3) protein levels in HFLFs were measured by Western blotting.


RESULTS
TGF-β1 increased the gene expression levels of Col1α1 and Col3α1, and promoted the phosphorylation levels of Smad2/3 protein in HFLFs. AM significantly reversed the expression level of Col3α1 mRNA, and inhibited p-Smad2/3 expression in HFLFs induced by TGF-β1.


CONCLUSIONS
AM could inhibit TGF-β1-induced the procollagen expression in cultured HFLFs possibly through the Smad2/3 signaling pathway.
Department of Respiratory Disease, Military General Hospital of Beijing PLA, Beijing 100700, China.
Shuling
S
Hao
Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Peking University, Beijing 100191, China.
Weiping
W
Wang
Department of Oncology Disease, Military General Hospital of Beijing PLA, Beijing 100700, China.
Zhonghe
Z
Yu
Department of Respiratory Disease, Military General Hospital of Beijing PLA, Beijing 100700, China.
Jiandong
J
Li
D053607:Adrenomedullin / Q000494:pharmacology*; D002478:Cells, Cultured; D005347:Fibroblasts / Q000378:metabolism; D006801:Humans; D008168:Lung / Q000166:cytology; D011347:Procollagen / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis; D015398:Signal Transduction / Q000187:drug effects*; D051899:Smad2 Protein / Q000502:physiology*; D051900:Smad3 Protein / Q000502:physiology*; D053773:Transforming Growth Factor beta1 / Q000037:antagonists & inhibitors* / Q000494:pharmacology
D011347:Procollagen; D012333:RNA, Messenger; C497782:SMAD2 protein, human; C497790:SMAD3 protein, human; D051899:Smad2 Protein; D051900:Smad3 Protein; D053773:Transforming Growth Factor beta1; D053607:Adrenomedullin