Identification of a new haplotype within the promoter region of the MSTN gene in horses from five of the most common breeds in Poland.
Myostatin (GDF-8) encoded by the MSTN gene is a negative regulator of muscle growth and development and belongs to the TGF-β superfamily of secreted growth and differentiation factors. In Thoroughbred horses, an MSTN sequence polymorphism (g.66493737C>T) is associated with optimum race distance. In the present study, a genetic polymorphism of a predicted promoter of the MSTN gene was investigated in 451 horses belonging to five different breeds: Arabian, Thoroughbred, Polish Konik, Hucul and Polish Heavy Draft. Two SNPs located at g.66495826T>C and g.66495696T>C (chr;18 EquCab 2.0) showed three haplotypes previously described: [g.66495826:T, g.66495696:T], [g.66495826:T, g.66495696:C], [g.66495826:C, g.66495696:T] with frequencies 0.877; 0.101; 0.005; respectively. Analysis performed on Polish Heavy Draft indicated the occurrence of a new haplotype [g.6649582626:C, g.66495696:C] with frequency 0.016.
Monika
M
Stefaniuk
Urszula
U
Kaczor
Romana
R
Augustyn
Artur
A
Gurgul
Maria
M
Kulisa
Zenon
Z
Podstawski
D000818:Animals; D005786:Gene Expression Regulation; D006239:Haplotypes; D006736:Horses / Q000235:genetics*; D055435:Myostatin / Q000235:genetics / Q000378:metabolism*; D011044:Poland; D011110:Polymorphism, Genetic; D011401:Promoter Regions, Genetic / Q000235:genetics*
D055435:Myostatin