Role of high mobility group box-1 and protection of growth hormone and somatostatin in severe acute pancreatitis.
In this study, we investigated the potential role of high-mobility group box 1 (HMGB1) in severe acute pancreatitis (SAP) and the effects of growth hormone (G) and somatostatin (S) in SAP rats. The rats were randomly divided into 6 groups of 20 each: sham-operated, SAP, SAP+saline, SAP+G, SAP+S and SAP+G+S. Ileum and pancreas tissues of rats in each group were evaluated histologically. HMGB1 mRNA expression was measured by reverse transcription-PCR. Levels of circulating TNF-α, IL-1, IL-6, and endotoxin were also measured. In the SAP group, interstitial congestion and edema, inflammatory cell infiltration, and interstitial hemorrhage occurred in ileum and pancreas tissues. The levels of HMGB1, TNF-α, IL-1, IL-6 and endotoxin were significantly up-regulated in the SAP group compared with those in the sham-operated group, and the 7-day survival rate was 0%. In the SAP+G and SAP+S groups, the inflammatory response of the morphological structures was alleviated, the levels of HMGB1, TNF-α, IL-1, IL-6, and endotoxin were significantly decreased compared with those in the SAP group, and the survival rate was increased. Moreover, in the SAP+G+S group, all histological scores were significantly improved and the survival rate was significantly higher compared with the SAP group. In conclusion, HMGB1 might participate in pancreas and ileum injury in SAP. Growth hormone and somatostatin might play a therapeutic role in the inflammatory response of SAP.
Department of Surgery, Huashan Hospital, Fudan University, Shanghai, China.
Y F
YF
Wang
Department of Surgery, Jinshan Pavilion Forest Hospital, Shanghai, China.
M
M
Wu
Department of Surgery, Huashan Hospital, Fudan University, Shanghai, China.
B J
BJ
Ma
Department of Surgery, Huashan Hospital, Fudan University, Shanghai, China.
D A
DA
Cai
Department of Surgery, Huashan Hospital, Fudan University, Shanghai, China.
B B
BB
Yin
D000818:Animals; D004487:Edema / Q000473:pathology; D004731:Endotoxins / Q000097:blood; D015870:Gene Expression; D013006:Growth Hormone / Q000378:metabolism*; D024243:HMGB1 Protein / Q000235:genetics / Q000378:metabolism*; D006406:Hematoma / Q000473:pathology; D007082:Ileum / Q000293:injuries / Q000473:pathology; D053583:Interleukin-1beta / Q000097:blood; D015850:Interleukin-6 / Q000097:blood; D008297:Male; D046529:Microscopy, Electron, Transmission; D020556:Neutrophil Infiltration / Q000502:physiology; D010179:Pancreas / Q000293:injuries / Q000378:metabolism / Q000473:pathology*; D019283:Pancreatitis, Acute Necrotizing / Q000209:etiology* / Q000378:metabolism / Q000473:pathology; D012333:RNA, Messenger / Q000302:isolation & purification; D011897:Random Allocation; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013004:Somatostatin / Q000378:metabolism*; D015996:Survival Rate; D014409:Tumor Necrosis Factor-alpha / Q000097:blood
D004731:Endotoxins; D024243:HMGB1 Protein; D053583:Interleukin-1beta; D015850:Interleukin-6; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; D013004:Somatostatin; D013006:Growth Hormone