Effects of elaidic acid, a predominant industrial trans fatty acid, on bacterial growth and cell surface hydrophobicity of lactobacilli.
The consumption of trans fatty acids (TFAs) increases the risk of cardiovascular diseases and coronary heart disease in human, and there are no effective ways to remove TFAs after consumption. The aim of this study was to investigate the effects of elaidic acid on bacterial growth, cell surface hydrophobicity of lactobacilli, and metabolism of elaidic acid by lactobacilli. Lactobacilli were inoculated in MRS broth containing 0, 100, 200, and 500 mg/L of elaidic acid. Viable cell counts of lactobacilli were enumerated, concentrations of elaidic acid were determined, and cell surface hydrophobicity of lactobacilli was measured. The results showed that the growth of lactobacilli was significantly inhibited by 500 mg/L of elaidic acid, however, a cell count of 8.50 log10 CFU/mL was still reached for tested lactobacilli after 24-h incubation. In particular, a reduction of elaidic acid was found for tested lactobacilli after 24-h incubation as compared to its initial concentration of 200 mg/L. However, cell surface hydrophobicity showed no correlations with the metabolism of elaidic acid by lactobacilli. Moreover, elaidic acid was able to influence cell surface hydrophobicity, and the decrease in hydrophobicity was more obvious in Lactobacillus paracasei and Lactobacillus casei compared with that in other tested lactobacilli. This study suggests that elaidic acid could change physiochemical surface properties of lactobacilli and the lactobacilli have the potential to reduce TFAs.
Food and Nutritional Science, School of Biological Sciences, The Univ. of Hong Kong, Pokfulam Road, Hong Kong.
Qinglong
Q
Wu
Nagendra P
NP
Shah
D001422:Bacterial Adhesion / Q000187:drug effects; D002462:Cell Membrane / Q000187:drug effects*; D015169:Colony Count, Microbial; D008401:Gas Chromatography-Mass Spectrometry; D006131:Growth Inhibitors / Q000494:pharmacology; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D007778:Lactobacillus / Q000187:drug effects* / Q000254:growth & development*; D007780:Lacticaseibacillus casei / Q000187:drug effects / Q000254:growth & development; D050296:Microbial Viability; D019301:Oleic Acid / Q000494:pharmacology*; D009829:Oleic Acids; D044242:Trans Fatty Acids / Q000494:pharmacology*
D006131:Growth Inhibitors; D009829:Oleic Acids; D044242:Trans Fatty Acids; D019301:Oleic Acid; C011459:elaidic acid
bacterial metabolism; cell surface hydrophobicity; elaidic acid; lactobacilli