Isolation and characterization of polymorphic microsatellite loci in Spondias radlkoferi (Anacardiaceae).
OBJECTIVE
Microsatellite markers were developed for Spondias radlkoferi to assess the impact of primate seed dispersal on the genetic diversity and structure of this important tree species of Anacardiaceae. •


RESULTS
Fourteen polymorphic loci were isolated from S. radlkoferi through 454 GS-FLX Titanium pyrosequencing of genomic DNA. The number of alleles ranged from three to 12. The observed and expected heterozygosities ranged from 0.382 to 1.00 and from 0.353 to 0.733, respectively. The amplification was also successful in S. mombin and two genera of Anacardiaceae: Rhus aromatica and Toxicodendron radicans. •


CONCLUSIONS
These microsatellite loci will be useful to assess the genetic diversity and population structure of S. radlkoferi and related species, and will allow us to investigate the effects of seed dispersal by spider monkeys (Ateles geoffroyi) on the genetic structure and diversity of S. radlkoferi populations in a fragmented rainforest.
Escuela Nacional de Estudios Superiores (ENES) Unidad Morelia, Universidad Nacional Autónoma de México (UNAM), Morelia, Michoacán, Mexico.
Esther
E
Aguilar-Barajas
Department of Ecology and Evolutionary Biology, University of California, Los Angeles, California USA.
Victoria L
VL
Sork
Instituto de Investigaciones Biológicas, Universidad Veracruzana, Xalapa, Veracruz, Mexico.
Arturo
A
González-Zamora
Centro de Investigaciones en Ecosistemas, UNAM, Morelia, Michoacán, Mexico.
Víctor
V
Rocha-Ramírez
Centro de Investigaciones en Ecosistemas, UNAM, Morelia, Michoacán, Mexico.
Víctor
V
Arroyo-Rodríguez
Escuela Nacional de Estudios Superiores (ENES) Unidad Morelia, Universidad Nacional Autónoma de México (UNAM), Morelia, Michoacán, Mexico ; Centro de Investigaciones en Ecosistemas, UNAM, Morelia, Michoacán, Mexico.
Ken
K
Oyama
Anacardiaceae; Rhus; Spondias; Toxicodendron; microsatellites