Nasal IL-17F is related to bronchial IL-17F/neutrophilia and exacerbations in stable atopic severe asthma.
Severe asthma (SA) is associated with neutrophil recruitment and T helper (TH )17 chemokine overexpression in bronchial biopsies. We aimed to evaluate IL-17A and IL-17F expression in nasal/bronchial lamina propria of atopic mild-to-severe asthmatics and controls in relation to neutrophilia and asthma exacerbations. Cryostat sections of nasal/bronchial biopsies obtained from 14 SA and 14 mild asthma (MA) stable atopic patients with rhinitis, and seven healthy controls were analyzed by immunohistochemistry for neutrophils, IL-17A and IL-17F expression. Atopic SA showed an increase in asthma exacerbations number, IL-17F and IL-17A expression in nasal/bronchial lamina propria compared to MA and controls, and a higher expression of bronchial neutrophils in SA compared to MA and controls. In all asthmatics, significant relationships were found between bronchial IL-17F and neutrophils/FEV1 , nasal IL-17F and bronchial neutrophil/IL-17 markers and between the latter and exacerbations, suggesting that nasal IL-17F might be informative on bronchial IL17-driven neutrophilia in atopic SA.
Department of Clinical and Biological Sciences, University of Torino, Torino, Italy.
V
V
Sorbello
G
G
Ciprandi
A
A
Di Stefano
G M
GM
Massaglia
G
G
Favatà
S
S
Conticello
M
M
Malerba
G
G
Folkerts
M
M
Profita
G
G
Rolla
F L M
FL
Ricciardolo
D000328:Adult; D001249:Asthma / Q000175:diagnosis* / Q000378:metabolism*; D001706:Biopsy; D001980:Bronchi / Q000378:metabolism / Q000473:pathology; D016022:Case-Control Studies; D018450:Disease Progression; D005260:Female; D005541:Forced Expiratory Volume; D006801:Humans; D006969:Hypersensitivity, Immediate / Q000175:diagnosis* / Q000378:metabolism*; D020381:Interleukin-17 / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D009297:Nasal Mucosa / Q000378:metabolism; D020556:Neutrophil Infiltration; D009504:Neutrophils / Q000378:metabolism*; D009666:Nose / Q000473:pathology; D020545:Respiratory Mucosa / Q000378:metabolism / Q000473:pathology; D012307:Risk Factors
D020381:Interleukin-17
IL-17; asthma; bronchial biopsy; nasal biopsy; neutrophils