Suppression of starvation-induced autophagy by recombinant toxic shock syndrome toxin-1 in epithelial cells.
Toxic shock syndrome toxin-1 (TSST-1), a superantigen produced from Staphylococcus aureus, has been reported to bind directly to unknown receptor(s) and penetrate into non-immune cells but its function is unclear. In this study, we demonstrated that recombinant TSST-1 suppresses autophagosomal accumulation in the autophagic-induced HeLa 229 cells. This suppression is shared by a superantigenic-deficient mutant of TSST-1 but not by staphylococcal enterotoxins, suggesting that autophagic suppression of TSST-1 is superantigenic-independent. Furthermore, we showed that TSST-1-producing S. aureus suppresses autophagy in the response of infected cells. Our data provides a novel function of TSST-1 in autophagic suppression which may contribute in staphylococcal persistence in host cells.
Department of Microbiology and Immunology, Hirosaki University Graduate School of Medicine, Hirosaki, Aomori, Japan.
Krisana
K
Asano
Department of Neuroanatomy, Cell Biology and Histology, Hirosaki University Graduate School of Medicine, Hirosaki, Aomori, Japan.
Yoshiya
Y
Asano
Department of Microbiology and Immunology, Hirosaki University Graduate School of Medicine, Hirosaki, Aomori, Japan.
Hisaya K
HK
Ono
Department of Microbiology and Immunology, Hirosaki University Graduate School of Medicine, Hirosaki, Aomori, Japan.
Akio
A
Nakane
D001343:Autophagy / Q000187:drug effects / Q000276:immunology*; D001427:Bacterial Toxins / Q000235:genetics / Q000494:pharmacology*; D015153:Blotting, Western; D016207:Cytokines / Q000378:metabolism; D004768:Enterotoxins / Q000235:genetics / Q000494:pharmacology*; D004847:Epithelial Cells / Q000276:immunology* / Q000473:pathology; D006367:HeLa Cells; D006801:Humans; D009154:Mutation / Q000235:genetics; D013203:Staphylococcal Infections / Q000188:drug therapy / Q000276:immunology* / Q000382:microbiology / Q000473:pathology; D013211:Staphylococcus aureus / Q000187:drug effects*; D013217:Starvation; D018089:Superantigens / Q000235:genetics / Q000494:pharmacology*
D001427:Bacterial Toxins; D016207:Cytokines; D004768:Enterotoxins; D018089:Superantigens; C030107:enterotoxin F, Staphylococcal