Interleukin-8 promotes human ovarian cancer cell migration by epithelial-mesenchymal transition induction in vitro.
OBJECTIVE
It has been well established that high serum levels of interleukin-8 (IL-8) in ovarian cancer result in a poor clinical outcome. Thus, the aim of this study was investigating the role of IL-8 in ovarian cancer development.


METHODS
Two human ovarian cancer cell lines (SKOV3 and OVCAR3) were cocultured with IL-8 (100 ng/L) for 24 h, then cell migration was determined by transwell assay. Epithelial-mesenchymal transition (EMT)-associated proteins including E-cadherin and β-catenin, and phosphorylation status of β-catenin were investigated by Western blot analysis.


RESULTS
After treatment with IL-8 (100 ng/L) for 24 h, transwell assay result showed that the number of migrated ovarian cells increased significantly. Western blot analysis revealed that protein levels of E-cadherin were decreased, while that of β-catenin were elevated both in IL-8 pretreated SKOV3 and OVCAR3 cells. We further found that phosphorylation status of β-catenin were elevated either in cytoplasm or in nucleus of these two ovarian cancer cell lines after treatment with IL-8 for 24 h.


CONCLUSIONS
Our data suggest that IL-8 induces EMT in ovarian cancer cells and implicates its potential role in enhancing ovarian cancer cell metastasis.
Department of Gynecology, The Ninth People's Hospital of Chongqing, 69 JiaLing Road, Chongqing, 400700, People's Republic of China.
J
J
Yin
F
F
Zeng
N
N
Wu
K
K
Kang
Z
Z
Yang
H
H
Yang
D015820:Cadherins / Q000378:metabolism; D045744:Cell Line, Tumor; D054443:Cell Migration Assays; D002465:Cell Movement / Q000187:drug effects*; D058750:Epithelial-Mesenchymal Transition / Q000187:drug effects*; D005260:Female; D006801:Humans; D016209:Interleukin-8 / Q000494:pharmacology*; D010051:Ovarian Neoplasms / Q000503:physiopathology*; D010766:Phosphorylation / Q000187:drug effects; D051176:beta Catenin / Q000378:metabolism
D015820:Cadherins; D016209:Interleukin-8; D051176:beta Catenin