Quantitative proteomic analysis of serum from pregnant women carrying a fetus with conotruncal heart defect using isobaric tags for relative and absolute quantitation (iTRAQ) labeling.
OBJECTIVE
To identify differentially expressed proteins from serum of pregnant women carrying a conotruncal heart defects (CTD) fetus, using proteomic analysis.


METHODS
The study was conducted using a nested case-control design. The 5473 maternal serum samples were collected at 14-18 weeks of gestation. The serum from 9 pregnant women carrying a CTD fetus, 10 with another CHD (ACHD) fetus, and 11 with a normal fetus were selected from the above samples, and analyzed by using isobaric tags for relative and absolute quantitation (iTRAQ) coupled with two-dimensional liquid chromatography-tandem mass spectrometry(2D LC-MS/MS). The differentially expressed proteins identified by iTRAQ were further validated with Western blot.


RESULTS
A total of 105 unique proteins present in the three groups were identified, and relative expression data were obtained for 92 of them with high confidence by employing the iTRAQ-based experiments. The downregulation of gelsolin in maternal serum of fetus with CTD was further verified by Western blot.


CONCLUSIONS
The identification of differentially expressed protein gelsolin in the serum of the pregnant women carrying a CTD fetus by using proteomic technology may be able to serve as a foundation to further explore the biomarker for detection of CTD fetus from the maternal serum.
Obstetrics and Gynecology Hospital, Fudan University, Shanghai, China.
Ying
Y
Zhang
Obstetrics and Gynecology Hospital, Fudan University, Shanghai, China.
Yuan
Y
Kang
Obstetrics and Gynecology Hospital, Fudan University, Shanghai, China.
Qiongjie
Q
Zhou
Obstetrics and Gynecology Hospital, Fudan University, Shanghai, China.
Jizi
J
Zhou
Children's Hospital, Fudan University, Shanghai, China.
Huijun
H
Wang
Department of Chemistry, Fudan University, Shanghai, China; Institute of Biomedicine, Fudan University, Shanghai, China.
Hong
H
Jin
Department of Chemistry, Fudan University, Shanghai, China.
Xiaohui
X
Liu
Key Laboratory of Molecular Medicine, Ministry of Education, Department of Biochemistry and Molecular Biology, Institute of Biomedical Sciences, Shanghai Medical College, Fudan University, Shanghai, China.
Duan
D
Ma
Obstetrics and Gynecology Hospital, Fudan University, Shanghai, China; Key Laboratory of Molecular Medicine, Ministry of Education, Department of Biochemistry and Molecular Biology, Institute of Biomedical Sciences, Shanghai Medical College, Fudan University, Shanghai, China; Shanghai Key Laboratory of Female Reproductive Endocrine Related Diseases, Shanghai, China.
Xiaotian
X
Li
D000328:Adult; D015415:Biomarkers / Q000097:blood*; D001798:Blood Proteins / Q000032:analysis*; D016022:Case-Control Studies; D002853:Chromatography, Liquid; D005260:Female; D018260:Gelsolin / Q000097:blood*; D020869:Gene Expression Profiling; D006330:Heart Defects, Congenital / Q000175:diagnosis* / Q000196:embryology / Q000378:metabolism; D006801:Humans; D011247:Pregnancy; D011296:Prenatal Diagnosis / Q000379:methods*; D040901:Proteomics; D053719:Tandem Mass Spectrometry
D015415:Biomarkers; D001798:Blood Proteins; D018260:Gelsolin