Genotyping of chloroquine resistant Plasmodium falciparum in wild caught Anopheles minimus mosquitoes in a malaria endemic area of Assam, India.
We validated the feasibility of using Plasmodium falciparum, the human malaria parasite, DNA present in wild caught vector mosquitoes for the characterization of chloroquine resistance status. House frequenting mosquitoes belonging to Anopheles minimus complex were collected from human dwellings in a malaria endemic area of Assam, Northeast India and DNA was extracted from the head-thorax region of individual mosquitoes. Anopheles minimus complex mosquitoes were identified to species level and screened for the presence of Plasmodium sp. using molecular tools. Nested PCR-RFLP method was used for genotyping of P. falciparum based on K76T mutation in the chloroquine resistance transporter (pfcrt) gene. Three of the 27 wild caught An. minimus mosquitoes were harbouring P. falciparum sporozoites (positivity 11.1%) and all 3 were had 76T mutation in the pfcrt gene, indicating chloroquine resistance. The approach of characterizing antimalarial resistance of malaria parasite in vector mosquitoes can potentially be used as a surveillance tool for monitoring transmission of antimalarial drug resistant parasite strains in the community.
Regional Medical Research Centre, NE Region (Indian Council of Medical Research), Post Box. No. 105, Dibrugarh - 786 001, Assam (India).
D K
DK
Sarma
Regional Medical Research Centre, NE Region (Indian Council of Medical Research), Post Box. No. 105, Dibrugarh - 786 001, Assam (India).
P K
PK
Mohapatra
Regional Medical Research Centre, NE Region (Indian Council of Medical Research), Post Box. No. 105, Dibrugarh - 786 001, Assam (India).
D R
DR
Bhattacharyya
Regional Medical Research Centre, NE Region (Indian Council of Medical Research), Post Box. No. 105, Dibrugarh - 786 001, Assam (India).
J
J
Mahanta
Regional Medical Research Centre, NE Region (Indian Council of Medical Research), Post Box. No. 105, Dibrugarh - 786 001, Assam (India).
A
A
Prakash
D000818:Animals; D000852:Anopheles / Q000145:classification / Q000235:genetics / Q000469:parasitology*; D000962:Antimalarials / Q000494:pharmacology*; D002738:Chloroquine / Q000494:pharmacology*; D016054:DNA, Protozoan / Q000235:genetics* / Q000302:isolation & purification; D004351:Drug Resistance; D019353:Endemic Diseases; D005787:Gene Frequency; D005838:Genotype; D060005:Genotyping Techniques; D006801:Humans; D007194:India / Q000453:epidemiology; D016778:Malaria, Falciparum / Q000453:epidemiology; D026901:Membrane Transport Proteins / Q000235:genetics; D020125:Mutation, Missense; D010963:Plasmodium falciparum / Q000145:classification / Q000187:drug effects* / Q000235:genetics* / Q000302:isolation & purification; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D015800:Protozoan Proteins / Q000235:genetics
D000962:Antimalarials; D016054:DNA, Protozoan; D026901:Membrane Transport Proteins; C416369:PfCRT protein, Plasmodium falciparum; D015800:Protozoan Proteins; D002738:Chloroquine