Potential conservation of circadian clock proteins in the phylum Nematoda as revealed by bioinformatic searches.
Although several circadian rhythms have been described in C. elegans, its molecular clock remains elusive. In this work we employed a novel bioinformatic approach, applying probabilistic methodologies, to search for circadian clock proteins of several of the best studied circadian model organisms of different taxa (Mus musculus, Drosophila melanogaster, Neurospora crassa, Arabidopsis thaliana and Synechoccocus elongatus) in the proteomes of C. elegans and other members of the phylum Nematoda. With this approach we found that the Nematoda contain proteins most related to the core and accessory proteins of the insect and mammalian clocks, which provide new insights into the nematode clock and the evolution of the circadian system.
Laboratorio de Cronobiología, Dto de Ciencia y Tecnología, Universidad Nacional de Quilmes, Bernal, Buenos Aires, Argentina.
Andrés
A
Romanowski
Laboratorio de Ingeniería Genética y Biología Celular y Molecular - Área Virosis de Insectos (LIGBCM - AVI), Instituto de Microbiología Básica y Aplicada (IMBA), Dto de Ciencia y Tecnología, Universidad Nacional de Quilmes, Bernal, Buenos Aires, Argentina.
Matías Javier
MJ
Garavaglia
Laboratorio de Cronobiología, Dto de Ciencia y Tecnología, Universidad Nacional de Quilmes, Bernal, Buenos Aires, Argentina.
María Eugenia
ME
Goya
Laboratorio de Ingeniería Genética y Biología Celular y Molecular - Área Virosis de Insectos (LIGBCM - AVI), Instituto de Microbiología Básica y Aplicada (IMBA), Dto de Ciencia y Tecnología, Universidad Nacional de Quilmes, Bernal, Buenos Aires, Argentina.
Pablo Daniel
PD
Ghiringhelli
Laboratorio de Cronobiología, Dto de Ciencia y Tecnología, Universidad Nacional de Quilmes, Bernal, Buenos Aires, Argentina.
Diego Andrés
DA
Golombek
D000818:Animals; D017360:Arabidopsis / Q000235:genetics; D056925:Circadian Rhythm Signaling Peptides and Proteins / Q000235:genetics*; D019295:Computational Biology / Q000379:methods*; D017124:Conserved Sequence / Q000235:genetics*; D004331:Drosophila melanogaster / Q000235:genetics; D051379:Mice; D015233:Models, Statistical; D009348:Nematoda / Q000235:genetics*; D009492:Neurospora crassa / Q000235:genetics; D013045:Species Specificity; D046940:Synechococcus / Q000235:genetics
D056925:Circadian Rhythm Signaling Peptides and Proteins