Association between a PD-1 gene polymorphism and antisperm antibody-related infertility in Iranian men.
OBJECTIVE
Programmed cell death-1 (PD-1, Pdcd1), an immunoreceptor belonging to the CD28/CTLA-4 family negatively regulates antigen receptor signalling by recruiting protein tyrosine phosphatase, SHP-2 upon interacting with either of two ligands, PD-L1 or PD-L2. This study investigates PD-1 gene polymorphism in patients with antisperm antibody-related infertility


METHODS
Genotyping was performed by polymerase chain reaction and restriction enzyme digestion (PCR-RFLP), this polymorphism was genotyped in 145 Iranian subjects (61 patients with antisperm antibody-related infertility and 84 healthy controls).


RESULTS
Patients frequencies of the G/A genotype in comparison with healthy controls (38.2 % vs. 32.7 %, OR =1.21, P = 0.35) were not significantly different. However, G/G and A/A genotype frequencies between patients and healthy controls were significantly different (P = 0.042, P = 0.00001, respectively). Also, allele frequencies of this polymorphism were significantly different (P = 0.0012) in patients compared to healthy controls.


CONCLUSIONS
According to these results, there is a correlation between PD-1 gene polymorphism and susceptibility to antisperm antibody-related infertility in our study group.
Department of Immunology and Biology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran, mr-zamani@razi.tums.ac.ir.
Mohammad Reza
MR
Zamani
Firouzeh Akbari
FA
Asbagh
Amir Hossein
AH
Massoud
Arash
A
Salmaninejad
Ahmad
A
Massoud
Nima
N
Rezaei
D000328:Adult; D000906:Antibodies / Q000235:genetics* / Q000276:immunology; D056726:Genetic Association Studies; D005838:Genotype; D006801:Humans; D007248:Infertility, Male / Q000235:genetics* / Q000473:pathology; D007492:Iran; D008297:Male; D020641:Polymorphism, Single Nucleotide; D061026:Programmed Cell Death 1 Receptor / Q000235:genetics*; D054592:Protein Tyrosine Phosphatase, Non-Receptor Type 11 / Q000235:genetics; D013094:Spermatozoa / Q000276:immunology / Q000473:pathology
D000906:Antibodies; C105992:PDCD1 protein, human; D061026:Programmed Cell Death 1 Receptor; C516724:PTPN11 protein, human; D054592:Protein Tyrosine Phosphatase, Non-Receptor Type 11