Antioxidant and anti-inflammatory effects of selenium in oral buccal mucosa and small intestinal mucosa during intestinal ischemia-reperfusion injury.
BACKGROUND
The aim of this study were to investigate whether selenium treatment attenuates lipid peroxidation and downregulates the NF-κB pathway in small intestinal mucosa and to examine whether the effect of selenium is also observed in oral buccal mucosa, during small intestinal IR injury.


METHODS
Eighteen rats were assigned into three groups: sham, IR, and IR + selenium. Saline or selenium was administered through a tail vein. 24 hours later, the superior mesenteric artery was exposed and clamped in the IR and IR + selenium groups. After ischemic and reperfusion period, animals were sacrificed and oral buccal mucosa and small intestinal mucosa were harvested.


RESULTS
Glutathione peroxidase activity and cytoplasmic IκB-α expression was higher in the IR + selenium group than that in the IR group. A malondialdehyde level, cytoplasmic phosphorylated inhibitor κB-α, nuclear NF-κB p65 expressions, and NF-κB p65 DNA-binding activity were lower in the IR + selenium group than those in the IR group.


CONCLUSIONS
A selenium treatment may cause increased GPx activity, attenuated lipid peroxidation, and downregulated the NF-κB pathway during small intestinal IR injury. Furthermore, these therapeutic benefits of selenium can be observed in oral buccal mucosa as well as small intestinal mucosa.
Department of Oral & Maxillofaical Surgery, School of Dentistry and Institute of Oral Bioscience, Research Institute of Chonbuk National University Hospital, Chonbuk National University, 664-14, Duckjindong, Chonju, 561-756 Chonbuk Korea.
Yongsoo
Y
Kim
Department of Oral & Maxillofaical Surgery, School of Dentistry and Institute of Oral Bioscience, Research Institute of Chonbuk National University Hospital, Chonbuk National University, 664-14, Duckjindong, Chonju, 561-756 Chonbuk Korea.
Dong Chil
DC
Kim
Department of Oral & Maxillofaical Surgery, School of Dentistry and Institute of Oral Bioscience, Research Institute of Chonbuk National University Hospital, Chonbuk National University, 664-14, Duckjindong, Chonju, 561-756 Chonbuk Korea.
Eui-Sic
ES
Cho
Department of Oral & Maxillofaical Surgery, School of Dentistry and Institute of Oral Bioscience, Research Institute of Chonbuk National University Hospital, Chonbuk National University, 664-14, Duckjindong, Chonju, 561-756 Chonbuk Korea.
Seung-O
SO
Ko
Department of Emergency Medicine, Seoul National University College of Medicine, 101 Daehak-Ro Jongno-Gu, Seoul, 110-744 Korea.
Woon Yong
WY
Kwon
Department of Emergency Medicine, Seoul National University College of Medicine, 101 Daehak-Ro Jongno-Gu, Seoul, 110-744 Korea.
Gil Joon
GJ
Suh
Department of Oral & Maxillofaical Surgery, School of Dentistry and Institute of Oral Bioscience, Research Institute of Chonbuk National University Hospital, Chonbuk National University, 664-14, Duckjindong, Chonju, 561-756 Chonbuk Korea.
Hyo-Keun
HK
Shin
Buccal mucosa; Ischemia reperfusion injury; Lipid peroxidation; NF kappaB; Selenium; Small intestine