Vascular smooth muscle cell culture in microfluidic devices.
This paper presents a microfluidic device enabling culture of vascular smooth muscle cells (VSMCs) where extracellular matrix coating, VSMC seeding, culture, and immunostaining are demonstrated in a tubing-free manner. By optimizing droplet volume differences between inlets and outlets of micro channels, VSMCs were evenly seeded into microfluidic devices. Furthermore, the effects of extracellular matrix (e.g., collagen, poly-l-Lysine (PLL), and fibronectin) on VSMC proliferation and phenotype expression were explored. As a platform technology, this microfluidic device may function as a new VSMC culture model enabling VSMC studies.
State Key Laboratory of Transducer Technology, Institute of Electronics , Chinese Academy of Sciences, Beijing, People's Republic of China.
Y C
YC
Wei
Department of Vascular Surgery, Clinical Division of Surgery, Chinese PLA General Hospital , Beijing, People's Republic of China.
F
F
Chen
T
T
Zhang
State Key Laboratory of Transducer Technology, Institute of Electronics , Chinese Academy of Sciences, Beijing, People's Republic of China.
D Y
DY
Chen
Department of Vascular Surgery, Clinical Division of Surgery, Chinese PLA General Hospital , Beijing, People's Republic of China.
X
X
Jia
State Key Laboratory of Transducer Technology, Institute of Electronics , Chinese Academy of Sciences, Beijing, People's Republic of China.
J B
JB
Wang
Department of Vascular Surgery, Clinical Division of Surgery, Chinese PLA General Hospital , Beijing, People's Republic of China.
W
W
Guo
State Key Laboratory of Transducer Technology, Institute of Electronics , Chinese Academy of Sciences, Beijing, People's Republic of China.
J
J
Chen