Comparison of 3 selective media for enumeration of Bacillus cereus in several food matrixes.
In this study, we compared the inclusivity, exclusivity, recoverability, and selectivity of the 3 selective agars (mannitol yolk polymyxin B agar [MYPA], polymyxin pyruvate egg yolk mannitol bromothymol blue agar [PEMBA], and Brillance Bacillus cereus agar [BBC agar]) for Bacillus cereus (B. cereus) from pure culture and several food samples. BBC agar showed greater exclusivity and selectivity in pure culture and in foods with high background flora, respectively; however, all the tested media showed similar recoverability (P > 0.05) of B. cereus in pure culture and in most foods. Our results suggest that BBC agar could be useful to enumerate B. cereus from, in particular, food matrixes with high background competing micro flora.
KU Center for Food Safety, Konkuk Univ, 1 Hwayang-dong, Gwangjin-gu, Seoul, 143-701, The Republic of Korea.
Jung-Whan
JW
Chon
Kwang-Young
KY
Song
Hyunsook
H
Kim
Kun-Ho
KH
Seo
D000362:Agar / Q000737:chemistry; D001409:Bacillus cereus / Q000254:growth & development / Q000302:isolation & purification*; D015169:Colony Count, Microbial; D003470:Culture Media / Q000737:chemistry*; D004530:Egg Yolk / Q000737:chemistry; D005504:Food Analysis; D005506:Food Contamination / Q000032:analysis; D005516:Food Microbiology; D061352:Pasteurization; D011112:Polymyxin B / Q000737:chemistry; D012680:Sensitivity and Specificity
D003470:Culture Media; D000362:Agar; D011112:Polymyxin B
Bacillus cereus; background microflora; comparison; food matrix; selective agars