Overproduction, crystallization and preliminary X-ray crystallographic analysis of Escherichia coli tRNA N6-threonylcarbamoyladenosine dehydratase.
Escherichia coli tRNA N6-threonylcarbamoyladenosine dehydratase (TcdA), previously called CsdL or YgdL, was overproduced and purified from E. coli and crystallized using polyethylene glycol 3350 as a crystallizing agent. X-ray diffraction data were collected to 2.70 Å resolution under cryoconditions using synchrotron X-rays. The crystals belonged to space group P2₁, with unit-cell parameters a=65.4, b=96.8, c=83.3 Å, β=111.7°. According to the Matthews coefficient, the asymmetric unit may contain up to four subunits of the monomeric protein, with a crystal volume per protein mass (VM) of 2.12 Å3 Da(-1) and 42.1% solvent content.
School of Systems Biomedical Science, Soongsil University, Seoul 156-743, Republic of Korea.
Sunmin
S
Kim
School of Systems Biomedical Science, Soongsil University, Seoul 156-743, Republic of Korea.
Keon Young
KY
Kim
School of Systems Biomedical Science, Soongsil University, Seoul 156-743, Republic of Korea.
Jeong Kuk
JK
Park
Biomolecular Function Research Branch, Division of Convergence Technology, Research Institute, National Cancer Center, Goyang, Gyeonggi 410-769, Republic of Korea.
Byung Il
BI
Lee
Department of Chemical Engineering, Soongsil University, Seoul 156-743, Republic of Korea.
Yun-Gon
YG
Kim
School of Systems Biomedical Science, Soongsil University, Seoul 156-743, Republic of Korea.
SangYoun
S
Park
D000241:Adenosine / Q000031:analogs & derivatives* / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics; D000595:Amino Acid Sequence; D003460:Crystallization; D018360:Crystallography, X-Ray; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics; D029968:Escherichia coli Proteins / Q000096:biosynthesis / Q000737:chemistry* / Q000235:genetics; D008969:Molecular Sequence Data; D012343:RNA, Transfer / Q000096:biosynthesis / Q000737:chemistry* / Q000235:genetics; D013913:Threonine Dehydratase / Q000096:biosynthesis / Q000737:chemistry* / Q000235:genetics
D029968:Escherichia coli Proteins; C003268:N(6)-(N-threonylcarbonyl)adenosine; D012343:RNA, Transfer; D013913:Threonine Dehydratase; D000241:Adenosine
CsdL; N6-threonylcarbamoyladenosine dehydratase; TcdA; tRNA hypermodification