The involvement of the Toll-like receptor signaling and Nrf2-Keap1 pathways in the in vitro regulation of IL-8 and HMOX1 for skin sensitization.
In vitro gene profiling studies have associated the molecular pathways of Nrf2-Keap1 and Toll-like receptor (TLR) signaling with skin sensitization. In this study, the role of these pathways in the regulation of protein biomarkers for skin sensitization was further elucidated using transient gene knock-down of key components of the signaling cascades in HaCaT cells after exposure to dinitrochlorobenzene (DNCB). The effect of targeting these pathways was established through evaluation of heme oxygenase1 (HMOX1) and interleukin (IL)-8 production. These experiments showed that Nrf2 is not involved in regulating HMOX1 after exposure to DNCB, but that activation of TLR signaling moderates the expression of HMOX1. The regulation of IL-8 depended on Nrf2, but also on the Toll/interleukin-1 receptor (TIR)-domain-containing adapter-inducing interferon-β (TRIF) adaptor protein in TLR signaling. This study provides new insights into the regulation of HMOX1 and IL-8, but the exact regulating mechanisms remain to be further elucidated.
a National Institute for Public Health and the Environment (RIVM) , Bilthoven , The Netherlands and.|b Department of Toxicogenomics , Maastricht University , Maastricht , The Netherlands.
Jochem W
JW
van der Veen
a National Institute for Public Health and the Environment (RIVM) , Bilthoven , The Netherlands and.
Ruthmila F
RF
Paskel
a National Institute for Public Health and the Environment (RIVM) , Bilthoven , The Netherlands and.
Noortje A M
NA
Smits
a National Institute for Public Health and the Environment (RIVM) , Bilthoven , The Netherlands and.
Henny
H
Hodemaekers
a National Institute for Public Health and the Environment (RIVM) , Bilthoven , The Netherlands and.|b Department of Toxicogenomics , Maastricht University , Maastricht , The Netherlands.
Henk
H
van Loveren
a National Institute for Public Health and the Environment (RIVM) , Bilthoven , The Netherlands and.
Janine
J
Ezendam
D033942:Adaptor Proteins, Vesicular Transport / Q000235:genetics / Q000378:metabolism; D015415:Biomarkers / Q000378:metabolism; D002460:Cell Line; D004137:Dinitrochlorobenzene / Q000276:immunology; D020869:Gene Expression Profiling; D051547:Heme Oxygenase-1 / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D007114:Immunization; D016209:Interleukin-8 / Q000235:genetics / Q000378:metabolism*; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics / Q000378:metabolism; D000072019:Kelch-Like ECH-Associated Protein 1; D015603:Keratinocytes / Q000502:physiology*; D053594:Myeloid Differentiation Factor 88 / Q000235:genetics / Q000378:metabolism; D051267:NF-E2-Related Factor 2 / Q000235:genetics / Q000378:metabolism*; D034741:RNA, Small Interfering / Q000235:genetics; D015398:Signal Transduction / Q000235:genetics; D012867:Skin / Q000276:immunology*; D051193:Toll-Like Receptors / Q000378:metabolism
D033942:Adaptor Proteins, Vesicular Transport; D015415:Biomarkers; D004137:Dinitrochlorobenzene; D016209:Interleukin-8; D047908:Intracellular Signaling Peptides and Proteins; C431364:KEAP1 protein, human; D000072019:Kelch-Like ECH-Associated Protein 1; D053594:Myeloid Differentiation Factor 88; D051267:NF-E2-Related Factor 2; C495635:NFE2L2 protein, human; D034741:RNA, Small Interfering; C470409:TICAM1 protein, human; D051193:Toll-Like Receptors; D051547:Heme Oxygenase-1
Heme oxygenase 1; IL-8; Nrf2-Keap1 pathway; immunotoxicology; siRNA; skin sensitization; toll-like receptor signaling