Reverse line blot hybridization with species-specific oligonucleotide probes: application to piroplasm detection.
Reverse line blot (RLB) hybridization has become a well-established and widely used method for the multiplex identification of several Babesia and Theileria species in hosts and tick vectors. The procedure is based on the simultaneous PCR amplification of a polymorphic region of the 18S rRNA gene from different piroplasms followed by identification of the individual species by hybridization to species-specific oligonucleotide probes covalently linked to a nylon membrane in a macroarray format.
Department of Animal Health, NEIKER-Instituto Vasco de Investigación y Desarrollo Agrario, Berreaga 1, 48160, Derio, Bizkaia, Spain, ahurtado@neiker.net.
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Hurtado
D000818:Animals; D001403:Babesia / Q000235:genetics*; D001404:Babesiosis / Q000175:diagnosis* / Q000469:parasitology*; D009693:Nucleic Acid Hybridization / Q000379:methods*; D015345:Oligonucleotide Probes; D016133:Polymerase Chain Reaction / Q000379:methods; D012337:RNA, Ribosomal, 18S / Q000235:genetics
D015345:Oligonucleotide Probes; D012337:RNA, Ribosomal, 18S