Influence of Carotino oil on in vitro rumen fermentation, metabolism and apparent biohydrogenation of fatty acids.
The study appraised the effects of Carotino oil on in vitro rumen fermentation, gas production, metabolism and apparent biohydrogenation of oleic, linoleic and linolenic acids. Carotino oil was added to a basal diet (50% concentrate and 50% oil palm frond) at the rate of 0, 2, 4, 6 and 8% dry matter of the diet. Rumen inoculum was obtained from three fistulated Boer bucks and incubated with 200 mg of each treatment for 24 h at 39°C. Gas production, fermentation kinetics, in vitro organic matter digestibility (IVOMD), volatile fatty acids (VFA), in vitro dry matter digestibility (IVDMD), metabolizable energy and free fatty acids were determined. Carotino oil did not affect (P > 0.05) gas production, metabolizable energy, pH, IVOMD, IVDMD, methane, total and individual VFAs. However, Carotino oil decreased (P < 0.05) the biohydrogenation of linoleic and linolenic acids but enhanced (P < 0.05) the biohydrogenation of oleic acid. After 24 h incubation, the concentrations of stearic, palmitic, pentadecanoic, myristic, myristoleic and lauric acids decreased (P < 0.05) while the concentration of linolenic, linoleic, oleic and transvaccenic acids and conjugated linoleic acid (CLAc9t11) increased (P < 0.05) with increasing levels of Carotino oil. Carotino oil seems to enhance the accumulation of beneficial unsaturated fatty acids without disrupting rumen fermentation.
Department of Animal Science, Faculty of Agriculture, Universiti Putra Malaysia, UPM Serdang, Selangor; Department of Animal Production, University of Ilorin, Ilorin, Nigeria.
Kazeem Dauda
KD
Adeyemi
Mahdi
M
Ebrahimi
Anjas Asmara
AA
Samsudin
Abd Razak
AR
Alimon
Roselina
R
Karim
Saiful Anuar
SA
Karsani
Awis Qurni
AQ
Sazili
D000821:Animal Feed; D000818:Animals; D003670:Deer; D004042:Dietary Fats, Unsaturated / Q000494:pharmacology*; D004063:Digestion / Q000187:drug effects / Q000502:physiology; D004734:Energy Metabolism / Q000187:drug effects; D005227:Fatty Acids / Q000378:metabolism*; D005231:Fatty Acids, Unsaturated / Q000378:metabolism; D005285:Fermentation / Q000187:drug effects*; D005740:Gases / Q000378:metabolism; D006865:Hydrogenation / Q000187:drug effects*; D066298:In Vitro Techniques; D008297:Male; D000073878:Palm Oil; D010938:Plant Oils / Q000494:pharmacology*; D012417:Rumen / Q000378:metabolism* / Q000382:microbiology
D004042:Dietary Fats, Unsaturated; D005227:Fatty Acids; D005231:Fatty Acids, Unsaturated; D005740:Gases; D010938:Plant Oils; D000073878:Palm Oil
biohydrogenation; carotino oil; rumen fermentation; unsaturated fatty acids