[Regional genotyping and the geographical distribution regarding Yersinia pestis isolates in China].
OBJECTIVE
To type Yersinia (Y.) pestis isolates under different regions (DFR) and to observe their geographical distributions in China.


METHODS
23 DFRs primers and PMT1 (plasmid) primer were used to verify the DFR genomovars of Y. pestiss strains from 11 plague foci in China. A total of 3 044 Y. pestis isolates were involved for analysis on DFR profiles with the characteristics of geographical distribution.


RESULTS
52 genomovars were verified in 3 044 Y. pestis strains in China in which 19 genomovars as major and 33 genomovars as minor genomovar. 21 new genomovars, namely genomovar 32 to genomovar 52 were described on the basis of 31 genomovars previously confirmed. Three new genomovars belonged to new major genomovars, namely Himalayan marmot natural plague foci of the Qinghai-Tibet plateau newly added genomovar 32 and genomovar 44 as major genomovars. Mongolian gerbil natural plague foci of Inner Mongolia plateau were newly added genomovar 50 as one of the major genomovars.


CONCLUSIONS
Among 21 new genomovars, 3 were major genomovars, with Chinese Y. pestis DFR as the major genomovars which had obvious distribution characteristics.
Plague Prevention and Control Department, Qinghai Institute for Endemic Diseases Prevention and Control, Xining 811602, China.
Xiaoyan
X
Yang
Plague Prevention and Control Department, Qinghai Institute for Endemic Diseases Prevention and Control, Xining 811602, China.
Baiqing
B
Wei
Plague Prevention and Control Department, Qinghai Institute for Endemic Diseases Prevention and Control, Xining 811602, China.
Juan
J
Jin
Plague Prevention and Control Department, Qinghai Institute for Endemic Diseases Prevention and Control, Xining 811602, China.
Cunxiang
C
Li
Plague Prevention and Control Department, Qinghai Institute for Endemic Diseases Prevention and Control, Xining 811602, China.
Haoming
H
Xiong
Plague Prevention and Control Department, Qinghai Institute for Endemic Diseases Prevention and Control, Xining 811602, China.
Youquan
Y
Xin
Plague Prevention and Control Department, Qinghai Institute for Endemic Diseases Prevention and Control, Xining 811602, China.
Rongjie
R
Wei
Plague Prevention and Control Department, Qinghai Institute for Endemic Diseases Prevention and Control, Xining 811602, China.
Meiying
M
Qi
Plague Prevention and Control Department, Qinghai Institute for Endemic Diseases Prevention and Control, Xining 811602, China.
Jian
J
He
Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences.
Ruifu
R
Yang
Plague Prevention and Control Department, Qinghai Institute for Endemic Diseases Prevention and Control, Xining 811602, China. Email: drx200907@163.com.
Ruixia
R
Dai
Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences. Email: songyajun88@gmail.com.
Yajun
Y
Song
D002681:China; D005838:Genotype; D005843:Geography; D015010:Yersinia pestis / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification