Detection of aneugenicity and clastogenicity in buccal epithelial cells of pan masala and gutkha users by pan-centromeric FISH analysis.
Chewing of betel quid, smoking and alcohol consumption are all associated with higher incidences of oral cancer. Genetic damage can be detected by fluorescent in situ hybridization (FISH) using human centromeric probes. In the present study FISH was performed on buccal epithelial cells of pan masala and gutkha chewers alone with and without additional tobacco smoking and/or alcohol consumption. The study comprised of 1500 male individuals. The present study found the highest frequency of micronuclei without a centromeric region (MN(-)) among gutkha users who also smoked and drank (P < 0.05). A significant increase in cells having micronuclei with a centromeric region (MN(+)) was observed among pan masala users who also smoked (P < 0.05). The study reveals that the clastogenic effects of pan masala/gutkha increase with smoking and alcohol consumption, but aneugenic effects were also observed among the pan masala chewers who smoked.
Department of Zoology, Section of Genetics, Faculty of Life Sciences, Aligarh Muslim University, Aligarh 202002, Uttar Pradesh, India, Department of Periodontics and Community Dentistry, Dr. Z.A. Dental College, Aligarh Muslim University, Aligarh 202002, Uttar Pradesh, India.
Smita
S
Jyoti
Department of Zoology, Section of Genetics, Faculty of Life Sciences, Aligarh Muslim University, Aligarh 202002, Uttar Pradesh, India, Department of Periodontics and Community Dentistry, Dr. Z.A. Dental College, Aligarh Muslim University, Aligarh 202002, Uttar Pradesh, India.
Falaq
F
Naz
Department of Zoology, Section of Genetics, Faculty of Life Sciences, Aligarh Muslim University, Aligarh 202002, Uttar Pradesh, India, Department of Periodontics and Community Dentistry, Dr. Z.A. Dental College, Aligarh Muslim University, Aligarh 202002, Uttar Pradesh, India.
Rahul
Department of Periodontics and Community Dentistry, Dr. Z.A. Dental College, Aligarh Muslim University, Aligarh 202002, Uttar Pradesh, India.
Saif
S
Khan
Department of Zoology, Section of Genetics, Faculty of Life Sciences, Aligarh Muslim University, Aligarh 202002, Uttar Pradesh, India, Department of Periodontics and Community Dentistry, Dr. Z.A. Dental College, Aligarh Muslim University, Aligarh 202002, Uttar Pradesh, India.
Fahad
F
Ali
Department of Zoology, Section of Genetics, Faculty of Life Sciences, Aligarh Muslim University, Aligarh 202002, Uttar Pradesh, India, Department of Periodontics and Community Dentistry, Dr. Z.A. Dental College, Aligarh Muslim University, Aligarh 202002, Uttar Pradesh, India.
Ambreen
A
Fatima
Department of Zoology, Section of Genetics, Faculty of Life Sciences, Aligarh Muslim University, Aligarh 202002, Uttar Pradesh, India, Department of Periodontics and Community Dentistry, Dr. Z.A. Dental College, Aligarh Muslim University, Aligarh 202002, Uttar Pradesh, India.
Saba
S
Khanam
Department of Zoology, Section of Genetics, Faculty of Life Sciences, Aligarh Muslim University, Aligarh 202002, Uttar Pradesh, India, Department of Periodontics and Community Dentistry, Dr. Z.A. Dental College, Aligarh Muslim University, Aligarh 202002, Uttar Pradesh, India yasir_hasansiddique@rediffmail.com.
Yasir Hasan
YH
Siddique
D000428:Alcohol Drinking / Q000009:adverse effects*; D024422:Aneugens / Q000633:toxicity*; D002503:Centromere; D004847:Epithelial Cells / Q000187:drug effects; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D048629:Micronuclei, Chromosome-Defective / Q000139:chemically induced*; D009061:Mouth Mucosa / Q000187:drug effects*; D009153:Mutagens / Q000633:toxicity*; D028321:Plant Preparations / Q000633:toxicity*; D012907:Smoking / Q000009:adverse effects*
D024422:Aneugens; D009153:Mutagens; D028321:Plant Preparations