Brain composition in Godyris zavaleta, a diurnal butterfly, Reflects an increased reliance on olfactory information.
Interspecific comparisons of brain structure can inform our functional understanding of brain regions, identify adaptations to species-specific ecologies, and explore what constrains adaptive changes in brain structure, and coevolution between functionally related structures. The value of such comparisons is enhanced when the species considered have known ecological differences. The Lepidoptera have long been a favored model in evolutionary biology, but to date descriptions of brain anatomy have largely focused on a few commonly used neurobiological model species. We describe the brain of Godyris zavaleta (Ithomiinae), a member of a subfamily of Neotropical butterflies with enhanced reliance on olfactory information. We demonstrate for the first time the presence of sexually dimorphic glomeruli within a distinct macroglomerular complex (MGC) in the antennal lobe of a diurnal butterfly. This presents a striking convergence with the well-known moth MGC, prompting a discussion of the potential mechanisms behind the independent evolution of specialized glomeruli. Interspecific analyses across four Lepidoptera further show that the relative size of sensory neuropils closely mirror interspecific variation in sensory ecology, with G. zavaleta displaying levels of sensory investment intermediate between the diurnal monarch butterfly (Danaus plexippus), which invests heavily in visual neuropil, and night-flying moths, which invest more in olfactory neuropil. We identify several traits that distinguish butterflies from moths, and several that distinguish D. plexippus and G. zavaleta. Our results illustrate that ecological selection pressures mold the structure of invertebrate brains, and exemplify how comparative analyses across ecologically divergent species can illuminate the functional significance of variation in brain structure.
Department of Genetics, Evolution & Environment, University College London, London, UK, WC1E 6BT.
Stephen H
SH
Montgomery
Swidbert R
SR
Ott
D000818:Animals; D057506:Arthropod Antennae / Q000033:anatomy & histology; D005075:Biological Evolution; D001921:Brain / Q000033:anatomy & histology*; D002080:Butterflies / Q000033:anatomy & histology*; D005260:Female; D008297:Male; D018613:Microscopy, Confocal; D024521:Mushroom Bodies / Q000033:anatomy & histology / Q000378:metabolism; D019581:Neuropil / Q000166:cytology*; D009833:Olfactory Pathways / Q000033:anatomy & histology*; D012727:Sex Characteristics; D018709:Statistics, Nonparametric; D016704:Synapsins / Q000378:metabolism; D014795:Visual Pathways / Q000033:anatomy & histology
D016704:Synapsins
Godyris zavaleta; Ithomiinae; Lepidoptera; adaptive brain evolution; antennal lobe; comparative neuroanatomy; macroglomerular complex; sexual dimorphism