hARIP2 is a putative growth-promoting factor involved in human colon tumorigenesis.
Activin is a multifunctional growth and differentiation factor of the growth factor-beta (TGF-β) superfamily, which inhibits the proliferation of colon cancer cells. It induces phosphorylation of intracellular signaling molecules (Smads) by interacting with its type I and type II receptors. Previous studies showed that human activin receptor-interacting protein 2 (hARIP2) can reduce activin signaling by interacting with activin type II receptors; however, the activity of hARIP2 in colon cancer has yet to be detailed. In vitro, overexpression of hARIP2 reduced activin-induced transcriptional activity and enhanced cell proliferation and colony formation in human colon cancer HCT8 cells and SW620 cells. Also, hARIP2 promoted colon cancer cell apoptosis, suggesting that a vital role in the initial stage of colon carcinogenesis. In vivo, immunohistochemistry revealed that hARIP2 was expressed more frequently and much more intensely in malignant colon tissues than in controls. These results indicate that hARIP2 is involved in human colon tumorigenesis and could be a predictive maker for colon carcinoma aggressiveness.
Changchun University of Science and Technology, Changchun, China E-mail : nszhang9@sina.com, hltiet@163.com, rumeng2215137@163.com.
Rui-Feng
RF
Gao
Zhan-Dong
ZD
Li
Jing
J
Jiang
Li-Hua
LH
Yang
Ke-Tong
KT
Zhu
Rui-Xin
RX
Lin
Hao
H
Li
Quan
Q
Zhao
Nai-Sheng
NS
Zhang
D028341:Activins / Q000235:genetics; D017209:Apoptosis / Q000235:genetics; D015153:Blotting, Western; D063646:Carcinogenesis / Q000235:genetics*; D049109:Cell Proliferation / Q000235:genetics; D003110:Colonic Neoplasms / Q000235:genetics* / Q000473:pathology; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D010766:Phosphorylation; D012334:RNA, Neoplasm / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D053475:Receptor-Interacting Protein Serine-Threonine Kinase 2 / Q000235:genetics*; D014162:Transfection; D016212:Transforming Growth Factor beta / Q000235:genetics / Q000378:metabolism*; D014407:Tumor Cells, Cultured
D012334:RNA, Neoplasm; D016212:Transforming Growth Factor beta; D028341:Activins; C506253:RIPK2 protein, human; D053475:Receptor-Interacting Protein Serine-Threonine Kinase 2