Conjugation of monocarboxybetaine molecules on amino-poly-p-xylylene films to reduce protein adsorption and cell adhesion.
A surface that resists protein adsorption and cell adhesion is highly desirable for many biomedical applications such as blood-contact devices and biosensors. In this study, we fabricated a carboxybetaine-containing surface and evaluated its antifouling efficacy. First, an amine-containing substrate was created by chemical vapor deposition of 4-aminomethyl-p-xylylene-co-p-xylylene (Amino-PPX). Aldehyde-ended carboxybetaine molecules were synthesized and conjugated onto Amino-PPX. The carboxybetaine-PPX surface greatly reduced protein adsorption and cell adhesion. The attachment of L929 cells on the carboxybetaine-PPX surface was reduced by 87% compared to the cell adhesion on Amino-PPX. Furthermore, RGD peptides could be conjugated on carboxybetaine-PPX to mediate specific cell adhesion. In conclusion, we demonstrate that a surface decoration with monocarboxybetaine molecules is useful for antifouling applications.
Department of Chemical Engineering, National Taiwan University , No. 1, Sec. 4,Roosevelt, Rd., Taipei 106, Taiwan.
Hsiu-Wen
HW
Chien
Ming-Chun
MC
Keng
Meng-Jiy
MJ
Wang
Hsien-Yeh
HY
Chen
Sheng-Tung
ST
Huang
Wei-Bor
WB
Tsai
D000327:Adsorption; D000814:Aniline Compounds / Q000737:chemistry*; D001622:Betaine / Q000737:chemistry*; D002448:Cell Adhesion / Q000187:drug effects; D011108:Polymers / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry*; D013499:Surface Properties
D000814:Aniline Compounds; D011108:Polymers; D011506:Proteins; C554824:poly(o-amino-p-xylylene-co-p-xylylene); D001622:Betaine