[STC-1 is involved in anti-hypoxia proliferative balance of renal cancer cells by down-regulation of intracellular Ca2+ and HIF-1α levels].
To investigate effects of stanniocalcin-1 (STC-1) on proliferation balance under hypoxic condition in renal cancer cells and its mechanism.



Hypoxic model was induced on renal cancer GRC-1 cells (Group H), the cells were treated with STC-1 protein at concentrations of 0.1 nmol/L (H1), 0.5 nmol/L (H2), 1.0 nmol/L (H3), or normal saline (H0) for 48 h， respectively. Cells proliferation was measured by MTT assay; mRNA and protein expressions of hypoxia inducible factor 1α (HIF-1α) and STC-1 in GRC-1 cells were detected by RT-PCR and ELISA, respectively; the intracellular levels of Ca2+ and adenosine triphosphate (ATP) were determined by fluorescence spectrophotometry and spectrophotometry, respectively.



The expression of HIF-1α, STC-1 and Ca2+ levels were increased in GRC-1 cells under hypoxia condition; STC-1 reversed these changes in a dose-effect manner. Hypoxia significantly inhibited cell proliferation and the generation of ATP in GRC-1 cells and exogenous STC-1 reversed the effects of hypoxia; ATP generation increased gradually with increasing STC-1 concentration, but the cell proliferation was reduced.



Exogenous STC-1 can promote the proliferation of renal cancer cells in hypoxia condition by reducing HIF-1α expression and Ca2+ content and increased ATP production, but the progressive inhibition of HIF-1 α hindered the renal carcinoma cell proliferation further, which indicates that STC-1 may be involved in anti-hypoxia proliferative balance of renal cancer cells.
Department of Urological Surgery，Affiliated Hospital of Guiyang Medical College，Guiyang 550004，China.
Zhi-hui
ZH
Zhu
Department of Urological Surgery，Affiliated Hospital of Guiyang Medical College，Guiyang 550004，China.
Jiang
J
Gu
Department of Urological Surgery，Affiliated Hospital of Guiyang Medical College，Guiyang 550004，China.
Yong-chun
YC
Zhang
Department of Urological Surgery，Affiliated Hospital of Guiyang Medical College，Guiyang 550004，China.
Qing-tao
QT
Yang
Department of Urological Surgery，Affiliated Hospital of Guiyang Medical College，Guiyang 550004，China.
Yong-an
YA
Yang
Department of Urological Surgery，Affiliated Hospital of Guiyang Medical College，Guiyang 550004，China.
Nan
N
Wang
Department of Urological Surgery，Jiangdu People's Hospital of Yangzhou，Yangzhou 225200，China.
Qing-liang
QL
Zhu
D002118:Calcium / Q000378:metabolism*; D015687:Cell Hypoxia; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015536:Down-Regulation; D006023:Glycoproteins / Q000378:metabolism*; D006801:Humans; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000378:metabolism*; D007680:Kidney Neoplasms / Q000473:pathology*; D012333:RNA, Messenger
D006023:Glycoproteins; C497442:HIF1A protein, human; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D012333:RNA, Messenger; C051795:teleocalcin; D002118:Calcium