Detection of koi herpesvirus (KHV) in healthy cyprinid seed stock.
Koi herpesvirus (KHV) disease is a lethal disease in common carp, an important food fish in Asian countries, the seed of which is used in restocking programs for freshwater fishery management. We inspected apparently healthy seed stock of common carp Cyprinus carpio L. and Siberian crucian carp Carassius auratus for the presence of KHV using PCR-based diagnostic tests as a part of a stock enhancement program from 2009 to 2010 in Korea. Consequently, KHV was detected from 24 of 232 inspections with yearly detection percentages of 5.2% in 2009 and 15.5% in 2010 using PCR primer sets for TK or SphI-5 as recommended by the OIE Manual of Diagnostic Tests for Aquatic Animals. Results indicate that the SphI-5 primer set was slightly more sensitive than the TK primer set, as shown by a higher detection rate. To determine the genotype of the KHV strains detected in this study, ORF40-specific PCR amplification was conducted, and the PCR products from 6 samples showed 100% nucleotide sequence identity with a Japanese strain (GenBank accession number AP008984) but not with US (DG657948) and Israeli strains (DG177346). This report conclusively demonstrated the presence of KHV in externally healthy seed of common carp and Siberian crucian carp, indicating a possible risk that subclinically infected seed stock can be released with a potential threat to wild populations.
Aquatic Life Disease Control Division, National Fisheries Research and Development Institute, Busan 619-902, Korea.
Mi-Young
MY
Cho
Kyoung-Mi
KM
Won
Jin-Woo
JW
Kim
Bo-Young
BY
Jee
Myoung-Ae
MA
Park
Suhee
S
Hong
D000818:Animals; D017756:Aquaculture; D001483:Base Sequence; D003530:Cyprinidae; D004279:DNA, Viral / Q000235:genetics / Q000302:isolation & purification; D005393:Fish Diseases / Q000821:virology*; D006564:Herpesviridae / Q000145:classification* / Q000302:isolation & purification*; D006566:Herpesviridae Infections / Q000662:veterinary*
D004279:DNA, Viral