[Expression of CCDC67 mRNA and its role in patients with papillary thyroid carcinoma].
OBJECTIVE
To explore the expression of CCDC67 mRNA between papillary thyroid carcinoma (PTC) and adjacent normal tissues.


METHODS
Reverse transcription-polymerase chain reaction (RT-PCR) was employed to detect the expression of CCDC67 mRNA in 48 cases of papillary thyroid carcinoma and their matched adjacent non-cancerous epithelium and cell lines of thyroid carcinoma.


RESULTS
There was no expression of CCDC67 mRNA in SW579 and TPC-1 cell line. All cases had the expression of CCDC67 in adjacent normal tissues while the expression of CCDC67 mRNA was down-regulated significantly, only 39.6% (19/48) in 48 cases of thyroid carcinoma. In PTC, the expression rates of CCDC67 mRNA in stage I-II and III-IV were 60% (12/20) and 25% (7/28) and 24.1% (7/29) and 63.2% (12/19) in the lymph node metastasis and non-metastasis groups respectively. Also the expression rates of CCDC67 mRNA in Grades I and II were 61.1% (11/18) and 26.7% (8/30) respectively. And there was significant difference between two groups (P < 0.05).


CONCLUSIONS
The down-regulated expression of CCDC67 is one of reasons for inactivation of PTC. And it may play an important role in carcinogenesis and progression of PTC.
Department of Thyroid Surgery, First Affiliated Hospital, Zhengzhou University, Key-Discipline Laboratory Clinical Medicine, Zhengzhou 450052, China. Email: detaoyin@zzu.edu.cn.
Detao
D
Yin
Jianhui
J
Xu
Yongfei
Y
Wang
Hongqiang
H
Li
Yan
Y
Zhang
Jinhua
J
Jiang
Qingduan
Q
Wang
D002277:Carcinoma; D002291:Carcinoma, Papillary; D002460:Cell Line; D018450:Disease Progression; D015536:Down-Regulation; D004848:Epithelium; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008207:Lymphatic Metastasis; D008869:Microtubule-Associated Proteins; D012333:RNA, Messenger; D000077273:Thyroid Cancer, Papillary; D013964:Thyroid Neoplasms; D025521:Tumor Suppressor Proteins
C576056:DEUP1 protein, human; D008869:Microtubule-Associated Proteins; D012333:RNA, Messenger; D025521:Tumor Suppressor Proteins