Determination of Aflatoxin M1 in Milk Powder by Ultrasonic-Assisted Extraction and Dispersive Solid-Phase Clean-up.
This work describes the application of ultrasound-assisted dispersive solid-phase extraction (UA-DSPE) as a sample preparation approach for aflatoxin M1 (AFM1) and also its subsequent determination by HPLC-fluorescence detection. A Box-Behnken design in combination with response surface methodology was implemented to determine the variables affecting the extraction procedure. The effects of different variables, including type and quantity of clean-up phase, ultrasonication time, ultrasonication temperature, nature and volume of the leaching solvent, were investigated in the optimization study. Primary secondary amine (PSA) and acetonitrile were selected as the clean-up phase and the leaching solvent, respectively. The obtained optimized values were 30 mg of PSA, 10 min ultrasonication time, 32°C ultrasonication temperature and 10 mL of acetonitrile. Under the optimal conditions, the limit of detection and limit of quantification were 0.0012 and 0.0045 µg kg(-1), respectively. The recoveries of the UA-DSPE procedure ranged from 80 to 92%, with relative standard deviations lower than 10% in all cases. Eventually, this method was successfully applied to the extraction of AFM1 in milk powder samples.
Department of Chemistry, Central Tehran Branch, Islamic Azad University, PO Box 14676-86831, Tehran, Iran dr.manoochehri@yahoo.com.
Mahboobeh
M
Manoochehri
Young Researchers and Elite Club, Karaj Branch, Islamic Azad University, PO Box 31485-313, Karaj, Iran.
Ali Akbar
AA
Asgharinezhad
Pirayeh Zist Laboratory, Qazvin, Iran.
Mahdi
M
Safaei
D016607:Aflatoxin M1 / Q000032:analysis*; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D057230:Limit of Detection; D016014:Linear Models; D008892:Milk / Q000737:chemistry*; D015233:Models, Statistical; D015203:Reproducibility of Results; D052616:Solid Phase Extraction / Q000379:methods*; D013010:Sonication
D016607:Aflatoxin M1