Preparation and characterization of novel PBAE/PLGA polymer blend microparticles for DNA vaccine delivery.
BACKGROUND
Poly(beta-amino ester) (PBAE) with its pH sensitiveness and Poly(lactic-co-glycolic acid) (PLGA) with huge DNA cargo capacity in combination prove to be highly efficient as DNA delivery system.


OBJECTIVE
To study the effectiveness of novel synthesized PBAE polymer with PLGA blend at different ratios in DNA vaccine delivery.


METHODS
In the present study, multifunctional polymer blend microparticles using a combination of PLGA and novel PBAE polymers A1 (bis(3-(propionyloxy)propyl)3,3'-(propane-1,3-diyl-bis(methylazanediyl))dipropanoate) and A2 (bis(4-(propionyloxy)butyl)3,3'-(ethane-1,2-diyl-bis(isopropylazanediyl))dipropanoate) at different ratios (85:15, 75:25, and 50:50) were prepared by double emulsion solvent removal method. The microparticles were characterized for cytotoxicity, transfection efficiency, and DNA encapsulation efficiency.


RESULTS
It was evident from results that among the microparticles prepared with PLGA/PBAE blend the PLGA:PBAE at 85:15 ratio was found to be more effective combination than the microparticles prepared with PLGA alone in terms of transfection efficiency and better DNA integrity. Microparticles made of PLGA and PBAE A1 at 85:15 ratio, respectively, were found to be less toxic when compared with microparticles prepared with A2 polymer.


CONCLUSIONS
The results encourage the use of the synthesized PBAE polymer in combination with PLGA as an effective gene delivery system.
Department of Pharmaceutical Biotechnology, Manipal College of Pharmaceutical Sciences, Manipal University, Manipal 576104, India.
Meenashi Vanathi
MV
Balashanmugam
Department of Pharmaceutical Biotechnology, Manipal College of Pharmaceutical Sciences, Manipal University, Manipal 576104, India ; College of Pharmacy-Boys, Shaqra University, Al Dawadmi Campus, Al Dawadmi 11911, Saudi Arabia.
Sivagurunathan
S
Nagarethinam
Department of Pharmaceutical Biotechnology, Manipal College of Pharmaceutical Sciences, Manipal University, Manipal 576104, India.
Hitesh
H
Jagani
Department of Pharmaceutical Biotechnology, Manipal College of Pharmaceutical Sciences, Manipal University, Manipal 576104, India.
Venkata Rao
VR
Josyula
College of Pharmacy-Boys, Shaqra University, Al Dawadmi Campus, Al Dawadmi 11911, Saudi Arabia.
Abdulmohsen
A
Alrohaimi
Directorate of Research, Manipal University, Manipal 576104, India.
Nayanabhirama
N
Udupa
D000818:Animals; D045744:Cell Line, Tumor; D018014:Gene Transfer Techniques; D019344:Lactic Acid / Q000008:administration & dosage* / Q000138:chemical synthesis; D051379:Mice; D008863:Microspheres; D011100:Polyglycolic Acid / Q000008:administration & dosage* / Q000138:chemical synthesis; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011108:Polymers / Q000008:administration & dosage* / Q000138:chemical synthesis; D019444:Vaccines, DNA / Q000008:administration & dosage* / Q000138:chemical synthesis
D011108:Polymers; D019444:Vaccines, DNA; C507253:poly(beta-amino ester); D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid