Ginsenoside-Rp1-induced apolipoprotein A-1 expression in the LoVo human colon cancer cell line.
BACKGROUND
Ginsenoside Rp1 (G-Rp1) is a novel ginsenoside derived from ginsenoside Rk1. This compound was reported to have anticancer, anti-platelet, and anti-inflammatory activities. In this study, we examined the molecular target of the antiproliferative and proapoptotic activities of G-Rp1.


METHODS
To examine the effects of G-Rp1, cell proliferation assays, propidium iodine staining, proteomic analysis by two-dimensional gel electrophoresis, immunoblotting analysis, and a knockdown strategy were used.


RESULTS
G-Rp1 dose-dependently suppressed the proliferation of colorectal cancer LoVo cells and increased their apoptosis. G-Rp1 markedly upregulated the protein level of apolipoprotein (Apo)-A1 in LoVo, SNU-407, DLD-1, SNU-638, AGS, KPL-4, and SK-BR-3 cells. The knockdown of Apo-A1 by its small-interfering RNA increased the levels of cleaved poly(ADP-ribose) polymerase and p53 and diminished the proliferation of LoVo cells.


CONCLUSIONS
These results suggest that G-Rp1 may act as an anticancer agent by strongly inhibiting cell proliferation and enhancing apoptosis through upregulation of Apo-A1.
Department of Bioinformatics and Life Science, Soongsil University, Seoul, Korea.
Mi-Yeon
MY
Kim
Research Institute and Hospital, National Cancer Center, Goyang, Korea.
Byong Chul
BC
Yoo
Department of Genetic Engineering, Sungkyunkwan University, Suwon, Korea.
Jae Youl
JY
Cho
Panax ginseng Meyer; anticancer effect; apolipoprotein A1; apoptosis; ginsenoside Rp1