Development and optimization of a non-enzymatic method of leukocyte isolation from macaque tissues.
METHODS
Cell isolation from macaque tissues involves laborious enzymatic digestion. The Medimachine provides a simpler, quicker non-enzymatic method, yielding 1.5–5 million cells/g of vaginal or rectal tissue from pigtailed macaques.


CONCLUSIONS
Flow cytometry analysis of the two methods revealed similar levels of cell viability and most major cell lineage and activation markers.
Lara E
LE
Pereira
Natalia
N
Makarova
Charles
C
Dobard
Rachael D
RD
Aubert
Priya
P
Srinivasan
Janet
J
McNicholl
James M
JM
Smith
D000818:Animals; D002469:Cell Separation / Q000379:methods*; D002470:Cell Survival; D005260:Female; D005434:Flow Cytometry; D007962:Leukocytes / Q000166:cytology* / Q000276:immunology / Q000378:metabolism; D008254:Macaca nemestrina / Q000502:physiology*; D012007:Rectum / Q000166:cytology; D014621:Vagina / Q000166:cytology