Protection of Angelica sinensis (Oliv) Diels against hepatotoxicity induced by Dioscorea bulbifera L. and its mechanism.
Dioscorea bulbifera L., a traditionally used medicinal plant in China, is reported to induce hepatotoxicity. The present study is designed to investigate the protection of an ethanol extract of Angelica sinensis (Oliv) Diels (AE) against an ethyl acetate fraction of D. bulbifera (EF)-induced liver injury and its engaged mechanism. High performance liquid chromatography (HPLC) analysis showed that the amount of diosbulbin B in EF was 16.03% and ferulic acid in AE was 0.18%. EF (350 mg/kg) increased serum alanine/aspartate aminotransferase (ALT/AST), alkaline phosphatase (ALP) activities and total bilirubin (TB) amount, while AE inhibited such an increase. Liver histological evaluation showed that AE prevented development of severe hepatic lesions induced by EF. Further results showed that EF decreased the expression of Bcl-2 and induced the cleaved activation of caspase-9 and -3, and all those effects were reversed by AE. AE also reversed EF-induced decreased expression of the inhibitor of kappa B (IκB), superoxide dismutase (SOD), and glutathione peroxidase (GPx). Taken together, our results demonstrate that AE can prevent EF-induced hepatotoxicity via preventing apoptosis, meanwhile IκB, SOD, and GPx may be involved in such protection.
The MOE Key Laboratory for Standardization of Chinese Medicines, The Shanghai Key Laboratory for Complex Prescription, and The SATCM Key Laboratory for New Resources and Quality Evaluation of Chinese Medicines, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine.
Chengwei
C
Niu
Junming
J
Wang
Lili
L
Ji
Zhengtao
Z
Wang
D029971:Angelica sinensis / Q000737:chemistry*; D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D015415:Biomarkers / Q000097:blood; D056486:Chemical and Drug Induced Liver Injury / Q000517:prevention & control*; D003373:Coumaric Acids / Q000378:metabolism; D027742:Dioscorea / Q000009:adverse effects*; D006576:Heterocyclic Compounds, 4 or More Rings / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008297:Male; D008519:Medicine, Traditional / Q000009:adverse effects; D008813:Mice, Inbred ICR; D018384:Oxidative Stress / Q000187:drug effects; D010936:Plant Extracts / Q000494:pharmacology*; D020011:Protective Agents / Q000494:pharmacology*; D013482:Superoxide Dismutase / Q000378:metabolism
D015415:Biomarkers; D003373:Coumaric Acids; D006576:Heterocyclic Compounds, 4 or More Rings; D010936:Plant Extracts; D020011:Protective Agents; C569454:diosbulbin B; C004999:ferulic acid; D013482:Superoxide Dismutase