Species-level assessment of secondary metabolite diversity amongHamigeraspecies and a taxonomic note on the genus.
Secondary metabolite phenotypes in nine species of theHamigeraclade were analysed to assess their correlations to a multi-gene species-level phylogeny. High-pressure-liquid-chromatography-based chemical analysis revealed three distinctive patterns of secondary metabolite production: (1) the nine species could be divided into two groups on the basis of production of the sesquiterpene tricinonoic acid; (2) the tricinonoic acid-producing group produced two cyclic peptides avellanins A and B; (3) the tricinonoic acid-non-producing group could be further divided into two groups according to the production of avellanins A and B. The chemical phenotype was consistent with the phylogeny of the species, although metabolite patterns were not diagnostic at the species level. In addition, the taxonomy of theHamigeraclade was updated with the new combinationHamigera ingelheimensisproposed forMerimbla ingelheimensis, so that all species in the clade are now in the same genus.
Department of Biotechnology and Biotechnology Research Center, Toyama Prefectural University , 5180 Kurokawa, Imizu , Toyama 939-0398 , Japan.
Yasuhiro
Y
Igarashi
Department of Biotechnology and Biotechnology Research Center, Toyama Prefectural University , 5180 Kurokawa, Imizu , Toyama 939-0398 , Japan.
Tomoaki
T
Hanafusa
Department of Biotechnology and Biotechnology Research Center, Toyama Prefectural University , 5180 Kurokawa, Imizu , Toyama 939-0398 , Japan.
Fumiya
F
Gohda
US Department of Agriculture, National Center for Agricultural Utilization Research , 1815 North University Street, Peoria , IL 61604 , USA.
Stephen
S
Peterson
Texas Therapeutics Institute, The Brown Foundation Institute of Molecular Medicine, University of Texas Health Science Center at Houston , 1881 East Road, Houston , TX 77054 , USA.
Gerald
G
Bills
Aspergillaceae; Eurotiomycetes; Hamigera ingelheimensis; chemotaxonomy; diversity; phylogeny; secondary metabolite