Human papillomavirus detection in head and neck squamous cell carcinoma.
BACKGROUND
Human Papillomavirus (HPV) has been associated with benign and malignant lesions in different epitheliums. The relationship between specific genotypes of high-risk HPV and some human cancers is well established. The aim of this work was to detect the HPV genotypes present in head and neck squamous cell carcinoma (HNSCC).


METHODS
We evaluated 71 samples of patients with histopathological diagnosis of HNSCC. The DNA extraction was conducted with the QIAGEN commercial kit. HPV detection and genotyping were performed by reverse hybridisation (INNO-LiPA) following the commercial specifications.


RESULTS
The mean age of the patients evaluated was 60.7 ± 13.11 years. The distribution of the lesions included 25 (35.20%) cases of squamous cell carcinoma (SCC) of the oral cavity, 23 (32.39%) of larynx, 16 (22.50%) of the oropharynx, 4 (5.63%) of paranasal sinus, and 2 (2. 80%) cases of SCC of the nostril. Of the patients, 78.9% were males, and of these 76% were tobacco users and 67.6% were alcohol consumers. The viral DNA was detected in 67.6% of the samples. The oral cavity and the larynx were the highest HPV-positivity sites with 35.40% and 29.10% respectively. The most frequent genotype was 16 as single infection (18.70%), or in combination with another HPV types. In the oral cavity and larynx the genotypes 16 or the combination 6 and 51 were present in 11.76% and 14.28%, respectively; and in the oropharynx the most frequent genotype was 16 in 22.50% of the cases, and in the paranasal sinus 50% presented infection with HPV-6. We observed that tumours with most advanced size and stage presented greater HPV positivity.


CONCLUSIONS
This study shows a high percentage of HPV positivity in SCC is mainly associated with high-risk HPV. It is important to highlight that viral infection, especially HPV-16, could be a risk factor in HNSCC progression.
Laboratorio de Genética Molecular, Instituto de Oncología y Hematología-MPPS, Caracas 1050, Venezuela.
Dayahindara
D
Vietía
Servicio de cabeza y cuello del Hospital Oncológico 'Padre Machado'-IVSS, Caracas 1050, Venezuela.
Juan
J
Liuzzi
Laboratorio de Genética Molecular, Instituto de Oncología y Hematología-MPPS, Caracas 1050, Venezuela.
Maira
M
Avila
Laboratorio de Genética Molecular, Instituto de Oncología y Hematología-MPPS, Caracas 1050, Venezuela.
Zoraya
Z
De Guglielmo
Laboratorio de Genética Molecular, Instituto de Oncología y Hematología-MPPS, Caracas 1050, Venezuela.
Yrneh
Y
Prado
Laboratorio de Genética Molecular, Instituto de Oncología y Hematología-MPPS, Caracas 1050, Venezuela.
María
M
Correnti
HPV; carcinoma; head; neck; squamous