Generation of iPS Cells from Human Peripheral Blood Mononuclear Cells Using Episomal Vectors.
Peripheral blood is the easy-to-access, minimally invasive, and the most abundant cell source to use for cell reprogramming. The episomal vector is among the best approaches for generating integration-free induced pluripotent stem (iPS) cells due to its simplicity and affordability. Here we describe the detailed protocol for the efficient generation of integration-free iPS cells from peripheral blood mononuclear cells. With this optimized protocol, one can readily generate hundreds of iPS cell colonies from 1 ml of peripheral blood.
Division of Regenerative Medicine MC1528B, Department of Medicine, Loma Linda University, 11234 Anderson Street, Loma Linda, CA, 92350, USA.
Ruijun Jeanna
RJ
Su
Division of Regenerative Medicine MC1528B, Department of Medicine, Loma Linda University, 11234 Anderson Street, Loma Linda, CA, 92350, USA.
Amanda
A
Neises
Division of Regenerative Medicine MC1528B, Department of Medicine, Loma Linda University, 11234 Anderson Street, Loma Linda, CA, 92350, USA. xzhang@llu.edu.
Xiao-Bing
XB
Zhang
D018929:Cell Culture Techniques / Q000379:methods; D002454:Cell Differentiation; D002478:Cells, Cultured; D065150:Cellular Reprogramming; D015870:Gene Expression; D005822:Genetic Vectors / Q000235:genetics*; D017919:Hepatitis B Virus, Woodchuck / Q000235:genetics; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology*; D007963:Leukocytes, Mononuclear / Q000166:cytology*; D010957:Plasmids / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics; D011994:Recombinant Proteins; D020218:Response Elements / Q000235:genetics; D013155:Spleen Focus-Forming Viruses / Q000235:genetics; D014162:Transfection; D051020:bcl-X Protein / Q000235:genetics / Q000502:physiology
C494811:BCL2L1 protein, human; D011994:Recombinant Proteins; D051020:bcl-X Protein
Episomal vectors; Hematopoietic cells; Human peripheral blood; Induced pluripotent stem cells; Integration-free; Mononuclear cells; Reprogramming