Modulation of Caenorhabditis elegans immune response and modification of Shigella endotoxin upon interaction.
To analyze the pathogenesis at both physiological and molecular level using the model organism, Caenorhabditis elegans at different developmental stages in response to Shigella spp. and its pathogen associated molecular patterns such as lipopolysaccharide. The solid plate and liquid culture-based infection assays revealed that Shigella spp. infects C. elegans and had an impact on the brood size and pharyngeal pumping rate. LPS of Shigella spp. was toxic to C. elegans. qPCR analysis revealed that host innate immune genes have been modulated upon Shigella spp. infections and its LPS challenges. Non-destructive analysis was performed to kinetically assess the alterations in LPS during interaction of Shigella spp. with C. elegans. The modulation of innate immune genes attributed the surrendering of host immune system to Shigella spp. by favoring the infection. LPS appeared to have a major role in Shigella-mediated pathogenesis and Shigella employs a tactic behavior of modifying its LPS content to escape from the recognition of host immune system.
Department of Biotechnology, Alagappa University, Karaikudi, Tamil Nadu, India.
Periyanaina
P
Kesika
Mani Iyer
MI
Prasanth
Krishnaswamy
K
Balamurugan
D000818:Animals; D017173:Caenorhabditis elegans / Q000235:genetics / Q000276:immunology* / Q000382:microbiology*; D054884:Host-Pathogen Interactions; D057131:Immune Evasion; D007113:Immunity, Innate; D008070:Lipopolysaccharides / Q000737:chemistry* / Q000276:immunology / Q000502:physiology*; D060888:Real-Time Polymerase Chain Reaction; D012760:Shigella / Q000472:pathogenicity*
D008070:Lipopolysaccharides
C-type lectins; C. elegans; Innate immunity; Lipopolysaccharides; Shigella