Differences in hormone localisation patterns of K and L type enteroendocrine cells in the mouse and pig small intestine and colon.
This study has investigated the patterns of colocalisation of the conventional K cell marker, glucagon-like insulinotropic peptide (GIP), and the L cell markers, glucagon like peptide-1 (GLP-1) and peptide YY (PYY), in enteroendocrine cells (EEC) of the small intestine and colon of mouse and pig. All combinations of the hormones, 3 in a cell, 2 in a cell and 1 at a time, were encountered. In both species, the three most common EEC types contained (1) both GLP-1 and PYY but not GIP, (2) GLP-1 alone or (3) GIP plus GLP-1 without PYY. Few GIP plus PYY cells and rare cells containing all 3 hormones were encountered. Gradients of cell types occurred along the intestine. For example, in mouse, there were no PYY cells in the duodenum and few in the jejunum, but >50% of labelled EEC in the distal ileum and colon were PYY immunoreactive. By contrast, over 40% of EEC in the pig duodenum contained PYY, and most also contained either GLP-1 or GIP. The gradient in pig was less pronounced. It is concluded that the traditional classification of K and L cells requires revision, and that there are major inter-species differences in the patterns of colocalisation of hormones that have been used to characterise K and L cells.
Department of Anatomy & Neuroscience, University of Melbourne, Parkville, VIC, 3010, Australia.
Hyun-Jung
HJ
Cho
Department of Anatomy & Neuroscience, University of Melbourne, Parkville, VIC, 3010, Australia.
Samin
S
Kosari
Department of Anatomy & Neuroscience, University of Melbourne, Parkville, VIC, 3010, Australia.
Billie
B
Hunne
Department of Anatomy & Neuroscience, University of Melbourne, Parkville, VIC, 3010, Australia.
Brid
B
Callaghan
Department of Anatomy & Neuroscience, University of Melbourne, Parkville, VIC, 3010, Australia.
Leni R
LR
Rivera
InVivo NSA, St Nolff, France.
David M
DM
Bravo
Department of Anatomy & Neuroscience, University of Melbourne, Parkville, VIC, 3010, Australia. j.furness@unimelb.edu.au.
John B
JB
Furness
D000818:Animals; D003106:Colon / Q000166:cytology* / Q000378:metabolism; D019858:Enteroendocrine Cells / Q000166:cytology* / Q000378:metabolism; D005749:Gastric Inhibitory Polypeptide / Q000378:metabolism; D052216:Glucagon-Like Peptide 1 / Q000378:metabolism; D006728:Hormones / Q000378:metabolism*; D007421:Intestine, Small / Q000166:cytology* / Q000378:metabolism; D008810:Mice, Inbred C57BL; D019894:Peptide YY / Q000378:metabolism; D034421:Sus scrofa
D006728:Hormones; D019894:Peptide YY; D005749:Gastric Inhibitory Polypeptide; D052216:Glucagon-Like Peptide 1