Hydroxyapatite-coated sillicone rubber enhanced cell adhesion and it may be through the interaction of EF1β and γ-actin.
Silicone rubber (SR) is a common soft tissue filler material used in plastic surgery. However, it presents a poor surface for cellular adhesion and suffers from poor biocompatibility. In contrast, hydroxyapatite (HA), a prominent component of animal bone and teeth, can promote improved cell compatibility, but HA is an unsuitable filler material because of the brittleness in mechanism. In this study, using a simple and economical method, two sizes of HA was applied to coat on SR to counteract the poor biocompatibility of SR. Surface and mechanical properties of SR and HA/SRs confirmed that coating with HA changes the surface topology and material properties. Analysis of cell proliferation and adhesion as well as measurement of the expression levels of adhesion related molecules indicated that HA-coated SR significantly increased cell compatibility. Furthermore, mass spectrometry proved that the biocompatibility improvement may be related to elongation factor 1-beta (EF1β)/γ-actin adjusted cytoskeletal rearrangement.
Department of Plastic and Cosmetic Surgery, Xinqiao Hospital, the Third Military Medical University, Chongqing, 400037, People's Republic of China.
Xiao-hua
XH
Shi
Department of Plastic and Cosmetic Surgery, Xinqiao Hospital, the Third Military Medical University, Chongqing, 400037, People's Republic of China.
Shao-liang
SL
Wang
Department of Plastic and Cosmetic Surgery, Xinqiao Hospital, the Third Military Medical University, Chongqing, 400037, People's Republic of China.
Yi-ming
YM
Zhang
Department of Plastic and Cosmetic Surgery, Chongqing Armed Police Corps Hospital, Chongqing, 400061, People's Republic of China.
Yi-cheng
YC
Wang
Department of War Trauma care, Hainan branch of PLA General Hospital, Sanya, Hainan, 572013, People's Republic of China.
Zhi
Z
Yang
Department of Plastic and Cosmetic Surgery, Xinqiao Hospital, the Third Military Medical University, Chongqing, 400037, People's Republic of China.
Xin
X
Zhou
Department of Plastic and Cosmetic Surgery, Xinqiao Hospital, the Third Military Medical University, Chongqing, 400037, People's Republic of China.
Ze-yuan
ZY
Lei
Department of Plastic and Cosmetic Surgery, Xinqiao Hospital, the Third Military Medical University, Chongqing, 400037, People's Republic of China.
Dong-li
DL
Fan
D000199:Actins / Q000096:biosynthesis / Q000378:metabolism*; D002448:Cell Adhesion / Q000502:physiology*; D015815:Cell Adhesion Molecules / Q000235:genetics / Q000378:metabolism; D049109:Cell Proliferation / Q000502:physiology; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible / Q000737:chemistry; D003599:Cytoskeleton / Q000378:metabolism; D017886:Durapatite / Q000737:chemistry*; D005347:Fibroblasts / Q000166:cytology; D006801:Humans; D008422:Materials Testing / Q000379:methods; D008855:Microscopy, Electron, Scanning; D020648:Peptide Elongation Factor 1 / Q000096:biosynthesis / Q000378:metabolism*; D012826:Silicone Elastomers / Q000737:chemistry*; D013499:Surface Properties
D000199:Actins; D015815:Cell Adhesion Molecules; D020099:Coated Materials, Biocompatible; D020648:Peptide Elongation Factor 1; D012826:Silicone Elastomers; D017886:Durapatite