Biodegradation of high concentrations of halomethanes by a fermentative enrichment culture.
A fermentative enrichment culture (designated DHM-1) that grows on corn syrup was evaluated for its ability to cometabolically biodegrade high concentrations of chloroform (CF), carbon tetrachloride (CT), and trichlorofluoromethane (CFC-11). When provided with corn syrup and vitamin B12 (0.03 mol B12 per mol CF), DHM-1 grew and biodegraded up to 2,000 mg/L of CF in 180 days, with only minor transient accumulation of dichloromethane and chloromethane. CT (15 mg/L) and CFC-11 (25 mg/L) were also biodegraded without significant accumulation of halomethane daughter products. The rate of CF biodegradation followed a Michaelis-Menten-like pattern with respect to the B12 concentration; one-half the maximum rate (66 mg CF/L/d) occurred at 0.005 mol B12 per mol CF. DHM-1 was able to biodegrade 500 mg/L of CF at an inoculum level as low as 10(-8) mg protein/L. The highest rate of CF biodegradation occurred at pH 7.7; activity decreased substantially below pH 6.0. DHM-1 biodegraded mixtures of CT, CFC-11, and CF, although CFC-11 inhibited CF biodegradation. Evidence for compete defluorination of CFC-11 was obtained based on a fluoride mass balance. Overall, the results suggest that DHM-1 may be effective for bioaugmentation in source zones contaminated with thousands of milligrams per liter of CF and tens of milligrams per liter of CT and CFC-11.
PeroxyChem Environmental Solutions (East Asia), Room 5B16, West Wing, Hanwei Plaza, 7 Guanghua Road, Chaoyang District, Beijing 100004, China.
Huifeng
H
Shan
Department of Environmental Engineering and Earth Sciences, Clemson University, Clemson, 29634-0919, SC, USA.
Han
H
Wang
Department of Environmental Engineering and Earth Sciences, Clemson University, Clemson, 29634-0919, SC, USA.
Rong
R
Yu
Department of Environmental Engineering and Earth Sciences, Clemson University, Clemson, 29634-0919, SC, USA.
Priya
P
Jacob
Department of Environmental Engineering and Earth Sciences, Clemson University, Clemson, 29634-0919, SC, USA.
David L
DL
Freedman
Bioremediation; Carbon tetrachloride; Chloroform; Trichlorofluoromethane; Vitamin B12