Hematopoietic stem/progenitor cell sources to generate reticulocytes for Plasmodium vivax culture.
The predilection of Plasmodium vivax (P. vivax) for reticulocytes is a major obstacle for its establishment in a long-term culture system, as this requires a continuous supply of large quantities of reticulocytes, representing only 1-2% of circulating red blood cells. We here compared the production of reticulocytes using an established in vitro culture system from three different sources of hematopoietic stem/progenitor cells (HSPC), i.e. umbilical cord blood (UCB), bone marrow (BM) and adult peripheral blood (PB). Compared to CD34+-enriched populations of PB and BM, CD34+-enriched populations of UCB produced the highest amount of reticulocytes that could be invaded by P. vivax. In addition, when CD34+-enriched cells were first expanded, a further extensive increase in reticulocytes was seen for UCB, to a lesser degree BM but not PB. As invasion by P. vivax was significantly better in reticulocytes generated in vitro, we also suggest that P. vivax may have a preference for invading immature reticulocytes, which should be confirmed in future studies.
Unit of Malariology, Institute of Tropical Medicine, Antwerp, Belgium.
Florian
F
Noulin
Department of development and regeneration, Stem Cell Institute, Leuven, Belgium.
Javed Karim
JK
Manesia
Unit of Malariology, Institute of Tropical Medicine, Antwerp, Belgium.
Anna
A
Rosanas-Urgell
Unit of Malariology, Institute of Tropical Medicine, Antwerp, Belgium.
Annette
A
Erhart
Unit of Malariology, Institute of Tropical Medicine, Antwerp, Belgium.
Céline
C
Borlon
Unit of Veterinary Protozoology, Institute of Tropical Medicine, Antwerp, Belgium.
Jan
J
Van Den Abbeele
Medical Research Council Unit, Fajara, The Gambia.
Umberto
U
d'Alessandro
Department of development and regeneration, Stem Cell Institute, Leuven, Belgium.
Catherine M
CM
Verfaillie
D015703:Antigens, CD / Q000378:metabolism; D018952:Antigens, CD34 / Q000378:metabolism; D001853:Bone Marrow; D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation; D002469:Cell Separation; D002478:Cells, Cultured; D005312:Fetal Blood / Q000166:cytology; D005434:Flow Cytometry; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000469:parasitology; D006801:Humans; D010966:Plasmodium vivax / Q000502:physiology*; D011990:Receptors, Transferrin / Q000378:metabolism; D012156:Reticulocytes / Q000166:cytology*
D015703:Antigens, CD; D018952:Antigens, CD34; C068126:CD71 antigen; D011990:Receptors, Transferrin