Effects of polychlorinated biphenyls 28, 30 and 118 on bovine spermatozoa in vitro.
Decline of semen quality due to endocrine-disrupting chemicals is of concern globally. Among endocrine disrupting chemicals (EDC), Polychlorinated biphenyls (PCB) are associated with reduced semen quality in various epidemiological studies. In this study, we evaluated the direct effects of selected PCBs (28, 30 and 118) on fresh spermatozoa of Simmetial bulls aged 2-4 years were evaluated in vitro by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and computer assisted semen analysis (CASA) (SCA; Microptics) analysis. IC50 values were found as 8.45, 5.45 and 9.55 ng/ml for PCB 28, 30 and 118, respectively. Total motility, progressive motility and viability decreased dependent on dose and duration of exposure (0, 2, 4 h). Total motility at IC50 doses decreased the most in PCB 28 (72.24%) followed by 30 (60.75%) and 118 (64.77%) at 2nd hour following exposure. Motility results were found to be in accordance with the vitality and morphology data where total abnormalities (especially reacted acrosome rate) were found to have increased.
Department of Pharmacology and Toxicology, Ankara University Faculty of Veterinary Medicine, Ankara, Turkey.
B
B
Yurdakok
K
K
Tekin
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Daskin
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A
Filazi
D000818:Animals; D002417:Cattle; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D052244:Endocrine Disruptors; D007928:Lethal Dose 50; D008297:Male; D011078:Polychlorinated Biphenyls / Q000494:pharmacology* / Q000633:toxicity; D055101:Semen Analysis / Q000662:veterinary; D013081:Sperm Motility / Q000187:drug effects; D013094:Spermatozoa / Q000187:drug effects*; D013778:Tetrazolium Salts; D013844:Thiazoles
D052244:Endocrine Disruptors; D013778:Tetrazolium Salts; D013844:Thiazoles; D011078:Polychlorinated Biphenyls; C022616:thiazolyl blue