Challenges of genotyping polyploid species.
Most plant species are known to be either ancient or recent polyploids, containing more than one genome as a result of past interspecific hybridization events (allopolyploidy) and/or genome doubling (autopolyploidy). Genotyping in polyploid species offers a set of unique challenges. Most molecular marker methodologies are made more complex by polyploidy, as multilocus alleles are generally produced when a single locus is targeted. Genotyping by sequencing is also more challenging in polyploids, with problematic assemblies of duplicated regions and difficulties in distinguishing between inter- and intragenomic polymorphisms. Strategies for identifying and overcoming the challenges of polyploidy in plant genotyping are proposed.
School of Agriculture and Food Sciences, University of Queensland, Brisbane, QLD, 4072, Australia, annaliese.mason@uq.edu.au.
Annaliese S
AS
Mason
D000483:Alleles; D005819:Genetic Markers; D005838:Genotype; D060005:Genotyping Techniques / Q000379:methods*; D010944:Plants / Q000235:genetics*; D011123:Polyploidy
D005819:Genetic Markers