Analysis of human papillomavirus and herpes simplex virus genus -2 from patients with cervical cancer in isfahan, iran.
BACKGROUND
Although cervical uterine cancer is a second form of cancer among women, but it occupies fifth form among all cancer types.


METHODS
In the present study, human papillomavirus (HPV) and herpes simplex virus 2 (HSV-2) in cervical cancer patients by using real time polymerase chain reaction (PCR) technique and the relation between their viral loads were investigated. 156 cervical carcinoma tissues were collected from married women in health centers in Isfahan, Iran.


RESULTS
The results showed that among 156 specimens, 58.97%, 45.51% and 7.05% were positive for HPV DNA, HPV-16 and HPV-18 respectively. Only in 2.3% specimens, HSV-2 and HPV-16 were positively detected where viral load HSV-2 in conjunction with HSV-16 dramatically increased.


CONCLUSIONS
Thus the present study not only confirmed that viral load of HPV-16 is more than other HPV types, but also in possible conjunction with HSV-2, both rates will significantly increase.
Department of Medicinal Biology, Zist Partak Institute, Academic Center for Education and Research, Isfahan, Iran.
Mehrnaz
M
Shanehsazzadeh
Zabol Medicinal Plants Research Center, Zabol University of Medical Sciences, Zabol, Iran ; Department of Pharmacognosy, Faculty of Pharmacy, Zabol University of Medical Sciences, Zabol, Iran.
Javad
J
Sharifi-Rad
Department of Medicinal Biology, Zist Partak Institute, Academic Center for Education and Research, Isfahan, Iran.
Mandana
M
Behbahani
Department of Medicinal Biology, Zist Partak Institute, Academic Center for Education and Research, Isfahan, Iran.
Abbasali
A
Pourazar
HPV; HPV16/18; HSV-2; cervical cancer; real time PCR