Stochastic 3D optical mapping by holographic localization of Brownian scatterers.
We present a wide-field microscopy technique for the 3D mapping of optical intensity distributions using Brownian gold nanopar-ticles as local probes, which are localized by off-axis holography. Fast computation methods allow us to localize hundreds of particles per minute with accuracies as good as 3 × 3 × 10nm³ for immobilized particles. Factors limiting this accuracy are discussed and the possibilities of the technique are illustrated through the 3D optical mapping of an evanescent and a propagative wave. Our results pave the way for a new stochastic imaging technique, well adapted to subwavelength optical characterization in water-based systems.
Ariadna
A
Martinez-Marrades
Jean-François
JF
Rupprecht
Michel
M
Gross
Gilles
G
Tessier
D006696:Holography / Q000379:methods*; D008027:Light; D008853:Microscopy / Q000379:methods*; D012542:Scattering, Radiation