Muriicola marianensis sp. nov., isolated from seawater.
A Gram-stain-negative, aerobic, orange-pigmented, rod-shaped and non-motile bacterium, designated strain A6B8(T), was isolated from seawater of the Mariana Trench. The isolate grew at 4-50 °C (optimum 30-35 °C), at pH 6.5-8.0 (optimum pH 7.5) and with 0.5-4.0 % (w/v) NaCl (optimum 1.0-2.0 %). Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain A6B8(T) was related most closely to the genus Muriicola and shared highest sequence similarity of 97.7 % with Muriicola jejuensis EM44(T). Chemotaxonomic analysis showed menaquinone 6 (MK-6) was the predominant isoprenoid and iso-C15 : 0, iso-C15 : 1 G and iso-C17 : 0 3-OH were the major cellular fatty acids. The polar lipid profile of strain A6B8(T) included phosphatidylethanolamine, three unidentified aminolipids and four unidentified lipids. The genomic DNA G+C content was 47.1 mol%. The DNA-DNA relatedness value (23.3 %) clearly demonstrated that strains A6B8(T) and M. jejuensis EM44(T) were representatives of two different species. Based on the phenotypic, phylogenetic and chemotaxonomic characterizations, A6B8(T) ( = CGMCC 1.12606(T) = KCTC 32436(T)) is considered to be the type strain of a novel species of the genus Muriicola, for which the name Muriicola marianensis sp. nov. is proposed.
College of Life Sciences, Zhejiang University, Hangzhou 310058, PR China.
Jing
J
Hu
College of Life Sciences, Zhejiang University, Hangzhou 310058, PR China.
Wei-Yan
WY
Zhang
School of Forestry and Biotechnology, Zhejiang Agriculture & Forestry University, Linan 311300, PR China.
Xin-Qi
XQ
Zhang
College of Life Sciences, Zhejiang University, Hangzhou 310058, PR China.
Hong-Cheng
College of Life Sciences, Zhejiang University, Hangzhou 310058, PR China.
Xu-Fen
XF
Zhu
College of Life Sciences, Zhejiang University, Hangzhou 310058, PR China.
Min
M
Wu
D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D041965:Flavobacteriaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010140:Pacific Ocean; D010714:Phosphatidylethanolamines / Q000737:chemistry; D010802:Phylogeny; D010858:Pigmentation; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
D004269:DNA, Bacterial; D005227:Fatty Acids; D010714:Phosphatidylethanolamines; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C483858:phosphatidylethanolamine; C042455:menaquinone 6