Foodborne bacterial pathogens recovered from contaminated shawarma meat in northern Jordan.
BACKGROUND
The purpose of the study was to isolate, identify, and determine the antimicrobial resistance of the bacterial pathogens recovered from shawarma (donair) sandwiches served to the public in Jordan.


METHODS
Bacterial contamination of 100 shawarma sandwiches with pathogenic bacteria was studied by culture on selective media, serology, PCR assay, and antimicrobial susceptibility testing.


RESULTS
One hundred and forty-five bacterial isolates were identified. The predominant species was Escherichia coli (28.3%), with six isolates of serotype O157:H7, followed by Salmonella spp. (25.5%). Higher contamination rates were found in chicken sandwiches. The majority of these bacteria expressed high resistance to several antimicrobials, especially tetracycline and streptomycin. Citrobacter freundii was isolated from 15.9% and Staphylococcus aureus was isolated from 8.3% of the sandwiches. The presence of these pathogens is of primary concern because some strains are capable of producing a heat-stable enterotoxin that causes food poisoning in humans, and should therefore be taken into account in risk assessment.


CONCLUSIONS
Results signify the importance of sustained surveillance of foodborne pathogens in shawarma sandwiches to minimize the risk of contamination. Availability of data on the isolated pathogens and modes of transmission in food from different countries would provide a common ground for reaching international agreement on food safety regulations.
Faculty of Applied Medical Sciences, Jordan University of Science and Technology, Irbid, Jordan. nimri@just.edu.jo.
Laila
L
Nimri
Fatina
F
Abu Al-Dahab
Raymond
R
Batchoun
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001419:Bacteria / Q000145:classification* / Q000187:drug effects / Q000302:isolation & purification*; D024881:Drug Resistance, Bacterial; D059022:Food Safety; D006801:Humans; D007597:Jordan; D008460:Meat / Q000382:microbiology*; D008826:Microbial Sensitivity Tests; D016133:Polymerase Chain Reaction; D012703:Serotyping
D000900:Anti-Bacterial Agents