Chemical compositions, chromatographic fingerprints and antioxidant activities of Andrographis Herba.
This paper describes the development of an HPLC-UV-MS method for quantitative determination of andrographolide and dehydroandrographolide in Andrographis Herba and establishment of its chromatographic fingerprint. The method was validated for linearity, limit of detection and quantification, inter- and intra-day precisions, repeatability, stability and recovery. All the validation results of quantitative determination and fingerprinting methods were satisfactory. The developed method was then applied to assay the contents of andrographolide and dehydroandrographolide and to acquire the fingerprints of all the collected Andrographis Herba samples. Furthermore, similarity analysis and principal component analysis were used to reveal the similarities and differences between the samples on the basis of the characteristic peaks. More importantly, the DPPH free radical-scavenging and ferric reducing capacities of the Andrographis Herba samples were assayed. By bivariate correlation analysis, we found that six compounds are positively correlated to DPPH free radical scavenging and ferric reducing capacities, and four compounds are negatively correlated to DPPH free radical scavenging and ferric reducing capacities.
Department of Chinese Pharmaceutical Sciences and Chinese Medicine Resources, College of Pharmacy, China Medical University, Taichung 40402, Taiwan.
Yang
Y
Zhao
Department of Nursing, Hsin Sheng College of Medical Care and Management, Taoyuan 32544, Taiwan.
Chun-Pin
CP
Kao
Department of Chinese Pharmaceutical Sciences and Chinese Medicine Resources, College of Pharmacy, China Medical University, Taichung 40402, Taiwan.
Kun-Chang
KC
Wu
Department of Chinese Pharmaceutical Sciences and Chinese Medicine Resources, College of Pharmacy, China Medical University, Taichung 40402, Taiwan.
Chi-Ren
CR
Liao
Department of Nursing, Hungkuang University, Taichung 43302, Taiwan. elaine@sunrise.hk.edu.tw.
Yu-Ling
YL
Ho
Department of Chinese Pharmaceutical Sciences and Chinese Medicine Resources, College of Pharmacy, China Medical University, Taichung 40402, Taiwan. yschang@mail.cmu.edu.tw.
Yuan-Shiun
YS
Chang
D035685:Andrographis / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D004224:Diterpenes / Q000032:analysis*; D016166:Free Radical Scavengers / Q000032:analysis*; D013058:Mass Spectrometry
D004224:Diterpenes; D016166:Free Radical Scavengers; C030419:andrographolide; C478098:dehydroandrographolide