Strontium fructose 1, 6-diphosphate alleviate cyclophosphamide-induced oligozoospermia by improving antioxidant and inhibiting testicular apoptosis via FAS/FASL pathway.
This study investigated the treatment effects of a new compound, strontium fructose 1, 6-diphosphate (FDP-Sr), in cyclophosphamide (CP)-induced oligozoospermia. FDP-Sr, with extra high-energy supply, could reverse male hypogonadism in the testis. Male Wistar rats were randomly divided into three groups: control group (vehicle treated), CP group and CP + FDP-Sr group. Both CP group and CP + FDP-Sr groups were orally administered CP (20 mg kg(-1) ) consecutively for the first 7 days to establish CP-induced testicular toxic models. Subsequently, CP group was given orally distilled water per day, whereas CP + FDP-Sr group was received FDP-Sr (200 mg kg(-1) ) for 49 days. Compared to the CP group, the FDP-Sr group showed significantly increased levels of serum testosterone, testis relative weights and epididymal sperm counts in rats. In addition, rats treated by FDP-Sr showed the recuperative activities of testicular marker enzymes and normalised levels of antioxidants in tissue. Testicular protection of FDP-Sr was further demonstrated by enhancing expression of P450scc, reducing ability of FAS/FASL and generating cytoprotection in the histopathological study. FDP-Sr appeared to possess an ability to attenuate CP-induced reproduction toxicity via the activation of antioxidants and steroidogenesis enzymes, and alleviate oligozoospermia via inhibition of testicular apoptosis by FAS/FASL pathway.
School of Life Sciences and Pharmaceutical Engineering, Nanjing Tech University, Nanjing, China.
J
J
Li
School of Pharmaceutical Sciences, Nanjing Tech University, Nanjing, China.
Y
Y
Hu
School of Pharmaceutical Sciences, Nanjing Tech University, Nanjing, China.
Q
Q
Zhang
School of Pharmaceutical Sciences, Nanjing Tech University, Nanjing, China.
B
B
Ma
School of Pharmacy, FMHS, The University of Auckland, Auckland, CA, USA.
Z
Z
Wu
School of Pharmaceutical Sciences, Nanjing Tech University, Nanjing, China.
Y
Y
Wang
School of Pharmaceutical Sciences, Nanjing Tech University, Nanjing, China.
J
J
Sun
School of Pharmaceutical Sciences, Nanjing Tech University, Nanjing, China.
J
J
Zhu
School of Life Sciences and Pharmaceutical Engineering, Nanjing Tech University, Nanjing, China.
H
H
Ying
School of Life Sciences and Pharmaceutical Engineering, Nanjing Tech University, Nanjing, China.
P
P
Ouyang
D000818:Animals; D018906:Antineoplastic Agents, Alkylating / Q000633:toxicity; D000975:Antioxidants / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects* / Q000235:genetics; D003520:Cyclophosphamide / Q000633:toxicity; D004195:Disease Models, Animal; D053222:Fas Ligand Protein / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D005635:Fructosediphosphates / Q000494:pharmacology*; D005978:Glutathione / Q000187:drug effects / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000187:drug effects / Q000378:metabolism; D007064:L-Iditol 2-Dehydrogenase / Q000187:drug effects / Q000378:metabolism; D007770:L-Lactate Dehydrogenase / Q000187:drug effects / Q000378:metabolism; D008054:Lipid Peroxides / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D009845:Oligospermia / Q000139:chemically induced / Q000378:metabolism*; D009929:Organ Size / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013076:Sperm Count; D053625:Sperm Retrieval; D013482:Superoxide Dismutase / Q000187:drug effects / Q000378:metabolism; D013737:Testis / Q000033:anatomy & histology / Q000187:drug effects*; D013739:Testosterone / Q000097:blood; D019014:fas Receptor / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D005723:gamma-Glutamyltransferase / Q000187:drug effects / Q000378:metabolism
D018906:Antineoplastic Agents, Alkylating; D000975:Antioxidants; D053222:Fas Ligand Protein; C491898:Fas protein, rat; C505662:Faslg protein, rat; D005635:Fructosediphosphates; D008054:Lipid Peroxides; D019014:fas Receptor; D013739:Testosterone; D008315:Malondialdehyde; D003520:Cyclophosphamide; D007064:L-Iditol 2-Dehydrogenase; D007770:L-Lactate Dehydrogenase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005723:gamma-Glutamyltransferase; D005978:Glutathione; C029063:fructose-1,6-diphosphate
Cyclophosphamide; oligozoospermia; strontium fructose 1, 6-diphosphate