Analysis of non-typeable Haemophilous influenzae VapC1 mutations reveals structural features required for toxicity and flexibility in the active site.
Bacteria have evolved mechanisms that allow them to survive in the face of a variety of stresses including nutrient deprivation, antibiotic challenge and engulfment by predator cells. A switch to dormancy represents one strategy that reduces energy utilization and can render cells resistant to compounds that kill growing bacteria. These persister cells pose a problem during treatment of infections with antibiotics, and dormancy mechanisms may contribute to latent infections. Many bacteria encode toxin-antitoxin (TA) gene pairs that play an important role in dormancy and the formation of persisters. VapBC gene pairs comprise the largest of the Type II TA systems in bacteria and they produce a VapC ribonuclease toxin whose activity is inhibited by the VapB antitoxin. Despite the importance of VapBC TA pairs in dormancy and persister formation, little information exists on the structural features of VapC proteins required for their toxic function in vivo. Studies reported here identified 17 single mutations that disrupt the function of VapC1 from non-typeable H. influenzae in vivo. 3-D modeling suggests that side chains affected by many of these mutations sit near the active site of the toxin protein. Phylogenetic comparisons and secondary mutagenesis indicate that VapC1 toxicity requires an alternative active site motif found in many proteobacteria. Expression of the antitoxin VapB1 counteracts the activity of VapC1 mutants partially defective for toxicity, indicating that the antitoxin binds these mutant proteins in vivo. These findings identify critical chemical features required for the biological function of VapC toxins and PIN-domain proteins.
Department of Microbiology and Immunology, University of Rochester Medical Center, Rochester, New York, United States of America.
Brooke
B
Hamilton
Department of Microbiology and Immunology, University of Rochester Medical Center, Rochester, New York, United States of America.
Alexander
A
Manzella
Department of Biochemistry and Biophysics, University of Rochester Medical Center, Rochester, New York, United States of America.
Karyn
K
Schmidt
Department of Microbiology and Immunology, University of Rochester Medical Center, Rochester, New York, United States of America.
Victoria
V
DiMarco
Department of Microbiology and Immunology, University of Rochester Medical Center, Rochester, New York, United States of America; Department of Biochemistry and Biophysics, University of Rochester Medical Center, Rochester, New York, United States of America; Center for RNA Biology, University of Rochester Medical Center, Rochester, New York, United States of America.
J Scott
JS
Butler
D000595:Amino Acid Sequence; D000992:Antitoxins / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics*; D001427:Bacterial Toxins / Q000235:genetics*; D020134:Catalytic Domain / Q000235:genetics*; D015870:Gene Expression / Q000235:genetics; D006193:Haemophilus influenzae / Q000235:genetics*; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics*; D017434:Protein Structure, Tertiary / Q000235:genetics; D012260:Ribonucleases / Q000235:genetics; D016415:Sequence Alignment
D000992:Antitoxins; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D012260:Ribonucleases