Monitoring changes of tumor microenvironment in colorectal submucosa using multiphoton microscopy.
Recently, targeting tumor microenvironment has become a novel approach for cancer therapy. Collagen is one of important components of tissue microenvironment, and has been considered as a new visible target for cancer therapy. In this work, multiphoton microscopy (MPM) was used to monitor the changes of collagen in tumor microenvironment during tumor progression. It was found that MPM facilitates imaging of tumor cells and collagen. MPM images in different tumor microenvironment during tumor progression shows obvious increase in cell number and collagen degration. In addition, the quantitative analysis of collagen content and orientation index in tumor microenvironment shows significant alteration during tumor progression. These results suggest that MPM has the ability to monitor the changes of collagen morphology in tumor microenvironment and quantify content and orientation index of collagen during tumor progression. Therefore this technique is a powerful imaging tool for the investigation of targeting tumor microenvironment for cancer therapy.
Institute of Laser and Optoelectronics Technology, Fujian Provincial Key Laboratory for Photonics Technology, Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, Fujian Normal University, Fuzhou, China.
Jingting
J
Qiu
Weizhong
W
Jiang
Yinghong
Y
Yang
Changyin
C
Feng
Zhifen
Z
Chen
Guoxian
G
Guan
Shuangmu
S
Zhuo
Jianxin
J
Chen
D003094:Collagen / Q000032:analysis*; D008853:Microscopy / Q000379:methods*; D009369:Neoplasms / Q000473:pathology*; D059016:Tumor Microenvironment
D003094:Collagen
collagen; colorectal cancer; multiphoton microscopy; second harmonic generation (SHG); tumor microenvironment; two-photon excited fluorescence (TPEF)