Spectra Optia granulocyte apheresis collections result in higher collection efficiency of viable, functional neutrophils in a randomized, crossover, multicenter trial.
BACKGROUND
Granulocyte transfusion from healthy donors is used in the treatment of patients with granulocyte function defects, or transient neutropenia and severe bacterial or fungal infections resistant to maximal antimicrobial treatment.


METHODS
This study evaluated the performance and safety of the newly developed granulocyte collection protocol of the Spectra Optia in a prospective, multicenter, open-label, randomized, paired crossover trial compared with the COBE Spectra apheresis system in a population of 32 evaluable healthy subjects. All subjects received granulocyte-colony-stimulating factor and dexamethasone before collection.


RESULTS
Granulocyte procedures from Spectra Optia apheresis procedures had an approximately 23% higher polymorphonuclear (PMN) collection efficiency (CE) than the COBE Spectra collections (mean, 53.7% vs. 43.2%; p < 0.01), while the platelet CE (10.9% vs. 10.8%, respectively) and hematocrit (7.4% vs. 7.4%) were comparable between collections on both devices. Spectra Optia collections generated a higher total PMN yield per liter of blood processed than those produced by the COBE Spectra (with means of 8.6 × 10(10) vs. 6.8 × 10(10) , respectively). Granulocyte viability was more than 99% with both devices, and chemotaxic and bacterial killing activities of circulating versus collected granulocytes were similarly preserved. Fewer operator adjustments were required with Spectra Optia and there was no significant difference in the number or intensity of adverse events between instruments.


CONCLUSIONS
CE of the granulocyte collection procedure with the Spectra Optia was approximately 10 percentage points higher than with the COBE Spectra, required less operator involvement, and is safe for clinical implementation.
Hoxworth Blood Center, University of Cincinnati, Cincinnati, Ohio.
Jose A
JA
Cancelas
Blood Center of Wisconsin, Milwaukee, Wisconsin.
Anand
A
Padmanabhan
Bonfils Blood Center, Denver, Colorado.
Tuan
T
Le
Children's Hospital Colorado, University of Colorado, Aurora, Colorado.
Daniel R
DR
Ambruso
Hoxworth Blood Center, University of Cincinnati, Cincinnati, Ohio.
Neeta
N
Rugg
Hoxworth Blood Center, University of Cincinnati, Cincinnati, Ohio.
D Nicole
DN
Worsham
Hoxworth Blood Center, University of Cincinnati, Cincinnati, Ohio.
Susan L
SL
Pinkard
Blood Center of Wisconsin, Milwaukee, Wisconsin.
Sharon
S
Graminske
Terumo BCT, Lakewood, Colorado.
Jennifer
J
Buck
Terumo BCT, Lakewood, Colorado.
Julie
J
Goldberg
Terumo BCT, Lakewood, Colorado.
Jerry
J
Bill
D001331:Automation; D015415:Biomarkers; D002470:Cell Survival; D002498:Centrifugation / Q000295:instrumentation; D002634:Chemotaxis, Leukocyte; D018592:Cross-Over Studies; D003907:Dexamethasone / Q000008:administration & dosage; D046148:Donor Selection; D004867:Equipment Design; D016179:Granulocyte Colony-Stimulating Factor / Q000008:administration & dosage; D006801:Humans; D007937:Leukapheresis / Q000295:instrumentation* / Q000379:methods; D007958:Leukocyte Count; D017708:Leukocyte Transfusion; D019520:Living Donors; D009503:Neutropenia / Q000628:therapy; D009504:Neutrophils / Q000276:immunology; D011446:Prospective Studies
D015415:Biomarkers; D016179:Granulocyte Colony-Stimulating Factor; D003907:Dexamethasone