An acidic pectin lyase from Aspergillus niger with favourable efficiency in fruit juice clarification.
The pectin lyase gene pnl-zj5a from Aspergillus niger ZJ5 was identified and expressed in Pichia pastoris. PNL-ZJ5A was purified by ultrafiltration, anion exchange and gel chromatography. The Km and Vmax values determined using citrus pectin were 0.66 mg ml(-1) and 32.6 μmol min(-1) mg(-1) , respectively. PNL-ZJ5A exhibited optimal activity at 43°C and retained activity over 25-50°C. PNL-ZJ5A was optimally active at pH 5 and effective in apple juice clarification. Compared with controls, PNL-ZJ5A increased the fruit juice yield significantly. Furthermore, PNL-ZJ5A reduced the viscosity of apple juice by 38.8% and increased its transmittance by 86.3%. PNL-ZJ5A combined with a commercial pectin esterase resulted in higher juice volume.
College of Life Sciences, Henan Agricultural University, Zhengzhou, China.
S X
SX
Xu
College of Life Sciences, Henan Agricultural University, Zhengzhou, China.|Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, China.
X
X
Qin
Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, China.
B
B
Liu
Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, China.
D Q
DQ
Zhang
Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, China.
W
W
Zhang
College of Life Sciences, Henan Agricultural University, Zhengzhou, China.
K
K
Wu
Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, China.
Y H
YH
Zhang
D001234:Aspergillus niger / Q000201:enzymology* / Q000235:genetics; D001628:Beverages; D002265:Carboxylic Ester Hydrolases / Q000378:metabolism; D003001:Cloning, Molecular; D005511:Food Handling / Q000379:methods*; D005638:Fruit; D005656:Fungal Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D005800:Genes, Fungal; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D027845:Malus; D010368:Pectins / Q000378:metabolism; D010843:Pichia / Q000235:genetics; D011133:Polysaccharide-Lyases / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D011994:Recombinant Proteins / Q000302:isolation & purification / Q000378:metabolism; D013379:Substrate Specificity; D014783:Viscosity
D005656:Fungal Proteins; D011994:Recombinant Proteins; D010368:Pectins; D002265:Carboxylic Ester Hydrolases; C022323:pectinesterase; D011133:Polysaccharide-Lyases; C027349:pectin lyase
Aspergillus niger; Pichia pastoris; juice clarification; pectin lyase; viscosity