A high-throughput cellulase screening system based on droplet microfluidics.
A new ultra-high-throughput screening assay for the detection of cellulase activity was developed based on microfluidic sorting. Cellulase activity is detected using a series of coupled enzymes leading to the formation of a fluorescent product that can be detected on a chip. Using this method, we have achieved up to 300-fold enrichments of the active population of cells and greater than 90% purity after just one sorting round. In addition, we proved that we can sort the cellulase-expressing cells from mixtures containing less than 1% active cells.
Raluca
R
Ostafe
Radivoje
R
Prodanovic
Department of Physics, School of Engineering and Applied Sciences, Harvard University , Cambridge, Massachusetts 02138, USA.
W
W
Lloyd Ung
Department of Physics, School of Engineering and Applied Sciences, Harvard University , Cambridge, Massachusetts 02138, USA.
David A
DA
Weitz
Rainer
R
Fischer