Functional investigation on aromatase in endometrial hyperplasia in polycystic ovary syndrome cases.
OBJECTIVE
To explore the possible significance of aromatase P450 in endometrial hyperplasia with a background of polycystic ovary syndrome (PCOS).


METHODS
Immunohistochemistry was used to determine the expression of aromatase P450 in endometrium of PCOS patients. Semiquantitative analysis of aromatase P450 expression of mRNA and protein level wasalso carried out by real-time quantitative RT-PCR method. After endometrial cells were stimulated by testosterone and letrozole in vitro, the estradiol (E2) level was determined, and the expression of cell aromatase P450 mRNA was assessed.


RESULTS
The aromatase P450 mRNA level was increased in endometria of PCOS patients. When endometrial cells were cultured with 10-6 M testosterone, the E2 level in the culture medium increased. An inhibitory effect on E2 generation and expression of aromatase P450 mRNA was observed when the endometrial cells were treated with 10(-5) M letrozole.


CONCLUSIONS
There is an increased expression of aromatase P450 in PCOS patient endometrium. Androgen stimulation could enhance the synthesis of aromatase P450 mRNA and the production of E2 in endometrial cells in vitro while letrozole could do the reverse.
Reproductive Center, Peking University Third Hospital, Beijing, China E-mail : jie.qiao@263.net.
Pan-Lin
PL
Zhao
Qiu-Fang
QF
Zhang
Li-Ying
LY
Yan
Shuo
S
Huang
Yuan
Y
Chen
Jie
J
Qiao
D000728:Androgens / Q000494:pharmacology; D001141:Aromatase / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D047072:Aromatase Inhibitors / Q000494:pharmacology; D015153:Blotting, Western; D016022:Case-Control Studies; D002478:Cells, Cultured; D004714:Endometrial Hyperplasia / Q000201:enzymology* / Q000235:genetics / Q000473:pathology; D004717:Endometrium / Q000201:enzymology*; D004958:Estradiol / Q000378:metabolism; D005260:Female; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects*; D006801:Humans; D007124:Immunoenzyme Techniques; D000077289:Letrozole; D009570:Nitriles / Q000494:pharmacology; D011085:Polycystic Ovary Syndrome / Q000201:enzymology* / Q000235:genetics / Q000473:pathology; D011379:Prognosis; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013739:Testosterone / Q000494:pharmacology; D014230:Triazoles / Q000494:pharmacology
D000728:Androgens; D047072:Aromatase Inhibitors; D009570:Nitriles; D012333:RNA, Messenger; D014230:Triazoles; D013739:Testosterone; D004958:Estradiol; D000077289:Letrozole; D001141:Aromatase