Spectroscopic approach for dynamic bioanalyte tracking with minimal concentration information.
Vibrational spectroscopy has emerged as a promising tool for non-invasive, multiplexed measurement of blood constituents - an outstanding problem in biophotonics. Here, we propose a novel analytical framework that enables spectroscopy-based longitudinal tracking of chemical concentration without necessitating extensive a priori concentration information. The principal idea is to employ a concentration space transformation acquired from the spectral information, where these estimates are used together with the concentration profiles generated from the system kinetic model. Using blood glucose monitoring by Raman spectroscopy as an illustrative example, we demonstrate the efficacy of the proposed approach as compared to conventional calibration methods. Specifically, our approach exhibits a 35% reduction in error over partial least squares regression when applied to a dataset acquired from human subjects undergoing glucose tolerance tests. This method offers a new route at screening gestational diabetes and opens doors for continuous process monitoring without sample perturbation at intermediate time points.
1] Laser Biomedical Research Center, Massachusetts Institute of Technology, Cambridge, MA 02139, USA [2] Department of Chemistry, School of Science and Technology, Kwansei Gakuin University, Sanda, Hyogo 669-1337, Japan.
Nicolas
N
Spegazzini
1] Department of Mechanical Engineering, Johns Hopkins University, Baltimore, Maryland 21218, USA [2] Department of Oncology, Johns Hopkins University, Baltimore, Maryland 21287, USA.
Ishan
I
Barman
Laser Biomedical Research Center, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
Narahara Chari
NC
Dingari
Laser Biomedical Research Center, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
Rishikesh
R
Pandey
Departamento de Física, Universidade Federal de Ouro Preto, Ouro Preto, MG 35400-000, Brazil.
Jaqueline S
JS
Soares
Department of Chemistry, School of Science and Technology, Kwansei Gakuin University, Sanda, Hyogo 669-1337, Japan.
Yukihiro
Y
Ozaki
Laser Biomedical Research Center, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
Ramachandra Rao
RR
Dasari
D001786:Blood Glucose / Q000032:analysis* / Q000378:metabolism; D004058:Diffusion; D005951:Glucose Tolerance Test; D006801:Humans; D007700:Kinetics; D016018:Least-Squares Analysis; D055095:Optics and Photonics / Q000295:instrumentation / Q000379:methods*; D012867:Skin / Q000098:blood supply; D013059:Spectrum Analysis, Raman / Q000295:instrumentation / Q000379:methods*; D014732:Vibration
D001786:Blood Glucose