Magnetic resonance imaging of post-ischemic blood-brain barrier damage with PEGylated iron oxide nanoparticles.
Blood-brain barrier (BBB) damage during ischemia may induce devastating consequences like cerebral edema and hemorrhagic transformation. This study presents a novel strategy for dynamically imaging of BBB damage with PEGylated supermagnetic iron oxide nanoparticles (SPIONs) as contrast agents. The employment of SPIONs as contrast agents made it possible to dynamically image the BBB permeability alterations and ischemic lesions simultaneously with T2-weighted MRI, and the monitoring could last up to 24 h with a single administration of PEGylated SPIONs in vivo. The ability of the PEGylated SPIONs to highlight BBB damage by MRI was demonstrated by the colocalization of PEGylated SPIONs with Gd-DTPA after intravenous injection of SPION-PEG/Gd-DTPA into a mouse. The immunohistochemical staining also confirmed the leakage of SPION-PEG from cerebral vessels into parenchyma. This study provides a novel and convenient route for imaging BBB alteration in the experimental ischemic stroke model.
Jiangsu Key Laboratory of Molecular and Functional Imaging, Department of Radiology, Medical School, Zhongda Hospital, Southeast University, No. 87, Dingjiaqiao, Nanjing 210009, China. gjteng@vip.sina.com.
Dong-Fang
DF
Liu
Cheng
C
Qian
Yan-Li
YL
An
Di
D
Chang
Sheng-Hong
SH
Ju
Gao-Jun
GJ
Teng
D000818:Animals; D001812:Blood-Brain Barrier / Q000473:pathology*; D002545:Brain Ischemia / Q000473:pathology*; D003287:Contrast Media / Q000138:chemical synthesis; D003911:Dextrans / Q000737:chemistry / Q000648:ultrastructure; D008279:Magnetic Resonance Imaging / Q000379:methods*; D058185:Magnetite Nanoparticles / Q000737:chemistry / Q000648:ultrastructure; D051379:Mice; D008810:Mice, Inbred C57BL; D053769:Nanocapsules / Q000737:chemistry / Q000648:ultrastructure; D010316:Particle Size; D011092:Polyethylene Glycols / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D003287:Contrast Media; D003911:Dextrans; D058185:Magnetite Nanoparticles; D053769:Nanocapsules; C097921:ferumoxtran-10; D011092:Polyethylene Glycols