Berteroin present in cruciferous vegetables exerts potent anti-inflammatory properties in murine macrophages and mouse skin.
Berteroin (5-methylthiopentyl isothiocyanate) is a sulforaphane analog present in cruciferous vegetables, including Chinese cabbage, rucola salad leaves, and mustard oil. We examined whether berteroin exerts anti-inflammatory activities using lipopolysaccharide (LPS)-stimulated Raw 264.7 macrophages and 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced mouse skin inflammation models. Berteroin decreased LPS-induced release of inflammatory mediators and pro-inflammatory cytokines in Raw 264.7 macrophages. Berteroin inhibited LPS-induced degradation of inhibitor of κBα (IκBα) and nuclear factor-κB p65 translocation to the nucleus and DNA binding activity. Furthermore, berteroin suppressed degradation of IL-1 receptor-associated kinase and phosphorylation of transforming growth factor β activated kinase-1. Berteroin also inhibited LPS-induced phosphorylation of p38 MAPK, ERK1/2, and AKT. In the mouse ear, berteroin effectively suppressed TPA-induced edema formation and down-regulated iNOS and COX-2 expression as well as phosphorylation of AKT and ERK1/2. These results demonstrate that berteroin exhibits potent anti-inflammatory properties and suggest that berteroin can be developed as a skin anti-inflammatory agent.
Department of Food Science and Nutrition, Hallym University, Chuncheon 200-702, Korea. betyloving@naver.com.
Yoo Jin
YJ
Jung
Department of Food Science and Nutrition, Hallym University, Chuncheon 200-702, Korea. wjdwodls@hallym.ac.kr.
Jae In
JI
Jung
WCU Biomodulation Major, Department of Agricultural Biotechnology and Center for Food and Bioconvergence, Seoul National University, Seoul 151-921, Korea. hanjini@snu.ac.kr.
Han Jin
HJ
Cho
Center for Food and Nutritional Genomics Research and Department of Food Science and Nutrition, Kyungpook National University, Daegu 702-701, Korea. mschoi@knu.ac.kr.
Myung-Sook
MS
Choi
Department of Food and Nutrition, Sookmyung Women's University, Seoul 140-742, Korea. mksung@sookmyung.ac.kr.
Mi-Kyung
MK
Sung
Department of Food Science and Nutrition, University of Ulsan, Ulsan 680-749, Korea. rinayu@ulsan.ac.kr.
Rina
R
Yu
Department of Food Science and Nutrition, Hallym University, Chuncheon 200-702, Korea. yhkang@hallym.ac.kr.
Young-Hee
YH
Kang
Department of Food Science and Nutrition, Hallym University, Chuncheon 200-702, Korea. jyoon@hallym.ac.kr.
Jung Han Yoon
JH
Park
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000494:pharmacology*; D051546:Cyclooxygenase 2 / Q000276:immunology; D016207:Cytokines / Q000276:immunology; D005260:Female; D020672:I-kappa B Proteins / Q000276:immunology; D007249:Inflammation / Q000139:chemically induced / Q000188:drug therapy* / Q000276:immunology; D017879:Isothiocyanates / Q000737:chemistry / Q000494:pharmacology*; D008070:Lipopolysaccharides / Q000276:immunology; D008264:Macrophages / Q000187:drug effects* / Q000276:immunology; D051379:Mice; D000072000:NF-KappaB Inhibitor alpha; D009569:Nitric Oxide / Q000276:immunology; D052247:Nitric Oxide Synthase Type II / Q000276:immunology; D012867:Skin / Q000187:drug effects* / Q000276:immunology; D013755:Tetradecanoylphorbol Acetate / Q000031:analogs & derivatives; D014675:Vegetables / Q000737:chemistry*
D000893:Anti-Inflammatory Agents; D016207:Cytokines; D020672:I-kappa B Proteins; D017879:Isothiocyanates; D008070:Lipopolysaccharides; C000606205:Nfkbia protein, mouse; C000599329:berteroin; D000072000:NF-KappaB Inhibitor alpha; D009569:Nitric Oxide; C020922:4-O-methyl-12-O-tetradecanoylphorbol 13-acetate; D052247:Nitric Oxide Synthase Type II; D051546:Cyclooxygenase 2; D013755:Tetradecanoylphorbol Acetate