Full-depth epidermis tomography using a Mirau-based full-field optical coherence tomography.
With a Gaussian-like broadband light source from high brightness Ce(3+):YAG single-clad crystal fiber, a full-field optical coherence tomography using a home-designed Mirau objective realized high quality images of in vivo and excised skin tissues. With a 40 × silicone-oil-immersion Mirau objective, the achieved spatial resolutions in axial and lateral directions were 0.9 and 0.51 μm, respectively. Such a high spatial resolution enables the separation of lamellar structure of the full epidermis in both the cross-sectional and en face planes. The number of layers of stratum corneum and its thickness were quantitatively measured. This label free and non-invasive optical probe could be useful for evaluating the water barrier of skin tissue in clinics. As a preliminary in vivo experiment, the blood vessel in dermis was also observed, and the flowing of the red blood cells and location of the melanocyte were traced.
Graduate Institute of Photonics and Optoelectronics, National Taiwan University, Taipei 10617, Taiwan.
Chien-Chung
CC
Tsai
Graduate Institute of Photonics and Optoelectronics, National Taiwan University, Taipei 10617, Taiwan.
Chia-Kai
CK
Chang
Graduate Institute of Photonics and Optoelectronics, National Taiwan University, Taipei 10617, Taiwan.
Kuang-Yu
KY
Hsu
Graduate Institute of Photonics and Optoelectronics, National Taiwan University, Taipei 10617, Taiwan.
Tuan-Shu
TS
Ho
Department of Dermatology, National Taiwan University Hospital and College of Medicine, National Taiwan University, Taipei, Taiwan.
Ming-Yi
MY
Lin
Department of Dermatology, National Taiwan University Hospital and College of Medicine, National Taiwan University, Taipei, Taiwan ; jengweitjiu@gmail.com.
Jeng-Wei
JW
Tjiu
Graduate Institute of Photonics and Optoelectronics, National Taiwan University, Taipei 10617, Taiwan ; Department of Electrical Engineering, National Taiwan University, Taipei 10617, Taiwan.
Sheng-Lung
SL
Huang
(060.2380) Fiber optics sources and detectors; (160.1435) Biomaterials; (170.3880) Medical and biological imaging; (170.4500) Optical coherence tomography; (180.3170) Interference microscopy