Effective targeting of DC-SIGN by α-fucosylamide functionalized gold nanoparticles.
Dendritic Cells (DCs), the most potent antigen-presenting cells, play a critical role in the detection of invading pathogens, which are recognized also by multiple carbohydrate-specific receptors. Among them, DC-SIGN is one of the best characterized, with high-mannose and Lewis-type glycan specificity. In this study, we present a potent DC-SIGN targeting device developed using gold nanoparticles functionalized with α-fucosyl-β-alanyl amide. The nanoparticles bound to cellular DC-SIGN and induced internalization as effectively as similar particles coated with comparable amounts of Lewis(X) oligosaccharide. They were found to be neutral toward dendritic cell maturation and IL-10 production, thus envisaging a possible use as targeted imaging tools and antigen delivery devices.
CNR-Institute of Molecular Science and Technologies (ISTM) , via Golgi 19, I-20133 Milan, Italy.
Daniela
D
Arosio
Fabrizio
F
Chiodo
José J
JJ
Reina
Marcello
M
Marelli
Soledad
S
Penadés
Yvette
Y
van Kooyk
Juan J
JJ
Garcia-Vallejo
Anna
A
Bernardi
D015815:Cell Adhesion Molecules / Q000378:metabolism*; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000378:metabolism*; D005643:Fucose / Q000031:analogs & derivatives* / Q000737:chemistry; D006046:Gold / Q000737:chemistry; D006801:Humans; D016753:Interleukin-10 / Q000378:metabolism; D037181:Lectins, C-Type / Q000378:metabolism*; D007983:Lewis Blood Group Antigens; D015335:Molecular Probes; D053758:Nanoparticles / Q000737:chemistry* / Q000378:metabolism*; D009844:Oligosaccharides / Q000737:chemistry; D011956:Receptors, Cell Surface / Q000378:metabolism*; D015091:beta-Alanine / Q000031:analogs & derivatives* / Q000737:chemistry; D000099215:DC-Specific ICAM-3 Grabbing Nonintegrin
D015815:Cell Adhesion Molecules; D005643:Fucose; D006046:Gold; D016753:Interleukin-10; D037181:Lectins, C-Type; D007983:Lewis Blood Group Antigens; D015335:Molecular Probes; D009844:Oligosaccharides; D011956:Receptors, Cell Surface; D015091:beta-Alanine; D000099215:DC-Specific ICAM-3 Grabbing Nonintegrin; C494219:Lewis a oligosaccharide; C000607323:fucosyl-beta-alanyl amide