Impact of electrospray ion source platforms on matrix effect due to plasma phospholipids in the determination of rivastigmine by LC-MS/MS.
BACKGROUND
This study evaluates the performance of three electrospray ionization source designs to monitor the interference of plasma phospholipids for reliable estimation of rivastigmine by LC-MS/MS for method ruggedness. The variation in the area response due to matrix effects was assessed by post-column infusion, post-extraction spiking and standard-line slope methods.


RESULTS
The observed interference due to coeluting phospholipids (m/z: 524.0/184.0) at the retention time of rivastigmine was 39.5, 12.9 and 0.4% using angular spray, orthogonal spray and dual orthogonal ion source spray design, respectively. Similarly, %CV for standard line slopes was 6.9, 4.6 and 2.0, respectively.


CONCLUSIONS
Z-spray source design provided better and efficient transfer of gas phase ions into the mass analyzer compared with angular and orthogonal spray. The study showed that Z-spray ion source provided minimum interference from phospholipids compared with other ion source designs.
Chemistry Department, Kadi Sarva Vishwavidyalaya, Sarva Vidyalaya Campus, Sector 15/23, Gandhinagar 382015, India.
Vikas
V
Trivedi
Vivek
V
Upadhyay
Manish
M
Yadav
Pranav S
PS
Shrivastav
Mallika
M
Sanyal
D001774:Blood Chemical Analysis / Q000379:methods*; D002853:Chromatography, Liquid; D006801:Humans; D048448:Phenylcarbamates / Q000097:blood* / Q000493:pharmacokinetics; D010743:Phospholipids / Q000097:blood*; D000068836:Rivastigmine; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods*; D013810:Therapeutic Equivalency
D048448:Phenylcarbamates; D010743:Phospholipids; D000068836:Rivastigmine