Genetic diversity and evolution of two capsid protein genes of citrus tristeza virus isolates from China.
The genetic diversity and population structure of citrus tristeza virus (CTV) isolates from China were investigated based on partial sequences spanning the C-terminal end of p61 and the complete sequences of the CPm and CP genes. Phylogenetic analysis revealed five known groups (RB, T30, T36, HA and VT) and one new group (VI) consisting of only Chinese CTV isolates. Incongruent phylogenetic trees coupled with recombination analysis suggested several recombination events in the CPm gene. Positive selection was detected at codon 9 of CPm and codons 31, 41 and 68 of CP. The widespread CTV subpopulation AT-1 found in China has a unique amino acid insertion at the C-terminus of p61, which could increase CTV population complexity with implications for the evolutionary history of the virus. Our results suggest relevant roles for gene flow, purifying selection and recombination in shaping the CTV population in China.
National Key Laboratory of Agromicrobiology, Huazhong Agricultural University, Wuhan, 430070, Hubei, China.
Guan-Wei
GW
Wu
Min
M
Tang
Guo-Ping
GP
Wang
Feng-Yin
FY
Jin
Zuo-Kun
ZK
Yang
Li-Jing
LJ
Cheng
Ni
N
Hong
D036022:Capsid Proteins / Q000235:genetics*; D002681:China; D002957:Citrus / Q000821:virology*; D017790:Closterovirus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D016000:Cluster Analysis; D019143:Evolution, Molecular; D051456:Gene Flow; D014644:Genetic Variation; D008969:Molecular Sequence Data; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology; D012367:RNA, Viral / Q000235:genetics; D011995:Recombination, Genetic; D012641:Selection, Genetic; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
D036022:Capsid Proteins; D012367:RNA, Viral