Allogeneic versus autologous derived cell sources for use in engineered bone-ligament-bone grafts in sheep anterior cruciate ligament repair.
The use of autografts versus allografts for anterior cruciate ligament (ACL) reconstruction is controversial. The current popular options for ACL reconstruction are patellar tendon or hamstring autografts, yet advances in allograft technologies have made allogeneic grafts a favorable option for repair tissue. Despite this, the mismatched biomechanical properties and risk of osteoarthritis resulting from the current graft technologies have prompted the investigation of new tissue sources for ACL reconstruction. Previous work by our lab has demonstrated that tissue-engineered bone-ligament-bone (BLB) constructs generated from an allogeneic cell source develop structural and functional properties similar to those of native ACL and vascular and neural structures that exceed those of autologous patellar tendon grafts. In this study, we investigated the effectiveness of our tissue-engineered ligament constructs fabricated from autologous versus allogeneic cell sources. Our preliminary results demonstrate that 6 months postimplantation, our tissue-engineered auto- and allogeneic BLB grafts show similar histological and mechanical outcomes indicating that the autologous grafts are a viable option for ACL reconstruction. These data indicate that our tissue-engineered autologous ligament graft could be used in clinical situations where immune rejection and disease transmission may preclude allograft use.
1 Department of Molecular and Integrative Physiology, University of Michigan , Ann Arbor, Michigan.
Vasudevan D
VD
Mahalingam
Nilofar
N
Behbahani-Nejad
Storm V
SV
Horine
Tyler J
TJ
Olsen
Michael J
MJ
Smietana
Edward M
EM
Wojtys
Deneen M
DM
Wellik
Ellen M
EM
Arruda
Lisa M
LM
Larkin
D000818:Animals; D016118:Anterior Cruciate Ligament / Q000601:surgery*; D059549:Anterior Cruciate Ligament Reconstruction; D016025:Bone Transplantation; D001842:Bone and Bones / Q000098:blood supply / Q000294:innervation; D003094:Collagen / Q000378:metabolism; D055119:Elastic Modulus; D004549:Elastin / Q000378:metabolism; D007593:Joint Instability; D007719:Knee Joint / Q000473:pathology / Q000503:physiopathology; D008022:Ligaments / Q000098:blood supply / Q000294:innervation / Q000637:transplantation*; D018919:Neovascularization, Physiologic; D012756:Sheep; D023822:Tissue Engineering / Q000379:methods*; D014182:Transplantation, Autologous; D014184:Transplantation, Homologous
D003094:Collagen; D004549:Elastin