Three photons are better than two.
Three-photon microscopy was suggested in the 1990s, but laser technology at the time was just not up to the challenge. Lauren Ware explores how recent technology advances are bringing three-photon microscopy back into focus.
Lauren Arcuri
LA
Ware
D000818:Animals; D001921:Brain / Q000166:cytology*; D001923:Brain Chemistry; D006801:Humans; D018613:Microscopy, Confocal / Q000379:methods; D008856:Microscopy, Fluorescence; D036641:Microscopy, Fluorescence, Multiphoton / Q000295:instrumentation / Q000379:methods* / Q000639:trends*; D017785:Photons