Intracellular signaling transduction pathways triggered by a well-known anti-GHR monoclonal antibody, Mab263, in vitro and in vivo.
A series of studies have reported that monoclonal antibody 263 (Mab263), a monoclonal antibody against the growth hormone receptor (GHR), acts as an agonist in vitro and in vivo. However, the intracellular signaling pathways triggered by Mab263 have not yet been delineated. Therefore, we examined the intracellular signaling pathways induced by Mab263 in vivo and in vitro in the present study. The results show that this antibody activated janus kinase 2 (JAK2), signal transducer and activator of transcription 3 (STAT3), STAT1 and extracellular signal-regulated kinase 1/2 (ERK1/2), but not STAT5. The phosphorylation kinetics of JAK2, STAT3/1 and ERK1/2 induced by Mab263 were subsequently analyzed in dose-response and time course experiments. Our observations indicate that Mab263 induced different intracellular signaling pathways than GH, which indicates that Mab263 is a signal-specific molecule and that Mab263 may be a valuable biological reagent to study the mechanism(s) of GHR-mediated intracellular signaling pathways.
College of Animal Science and Technology, Jilin Agricultural University, Xincheng Street 2888, Changchun 130118, China. lan2012172@163.com.
Hainan
H
Lan
State Key Laboratory of Animal Nutrition, China Agricultural University, Beijing 100193, China. weiv_li@hotmail.com.
Wei
W
Li
College of Animal Science and Technology, Jilin Agricultural University, Xincheng Street 2888, Changchun 130118, China. lanbotonglan@163.com.
Hailong
H
Jiang
College of Animal Science and Technology, Jilin Agricultural University, Xincheng Street 2888, Changchun 130118, China. lilantougao@163.com.
Yanhong
Y
Yang
College of Animal Science and Technology, Jilin Agricultural University, Xincheng Street 2888, Changchun 130118, China. zhengtougao@163.com.
Xin
X
Zheng
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology / Q000378:metabolism*; D016466:CHO Cells; D002460:Cell Line; D003412:Cricetulus; D004789:Enzyme Activation; D053614:Janus Kinase 2 / Q000378:metabolism*; D051379:Mice; D019950:Mitogen-Activated Protein Kinase 1 / Q000378:metabolism; D048052:Mitogen-Activated Protein Kinase 3 / Q000378:metabolism; D010766:Phosphorylation; D051381:Rats; D011986:Receptors, Somatotropin / Q000276:immunology / Q000378:metabolism*; D050794:STAT1 Transcription Factor / Q000378:metabolism*; D050796:STAT3 Transcription Factor / Q000378:metabolism*; D015398:Signal Transduction
D000911:Antibodies, Monoclonal; D011986:Receptors, Somatotropin; D050794:STAT1 Transcription Factor; D050796:STAT3 Transcription Factor; D053614:Janus Kinase 2; D019950:Mitogen-Activated Protein Kinase 1; D048052:Mitogen-Activated Protein Kinase 3