Nanoparticle-mediated binning and profiling of heterogeneous circulating tumor cell subpopulations.
The analysis of circulating tumor cells (CTCs) is an important capability that may lead to new approaches for cancer management. CTC capture devices developed to date isolate a bulk population of CTCs and do not differentiate subpopulations that may have varying phenotypes with different levels of clinical relevance. Here, we present a new device for CTC spatial sorting and profiling that sequesters blood-borne tumor cells with different phenotypes into discrete spatial bins. Validation data are presented showing that cancer cell lines with varying surface expression generate different binning profiles within the device. Working with patient blood samples, we obtain profiles that elucidate the heterogeneity of CTC populations present in cancer patients and also report on the status of CTCs within the epithelial-to-mesenchymal transition (EMT).
Leslie Dan Faculty of Pharmacy, University of Toronto, Toronto, ON (Canada).
Reza M
RM
Mohamadi
Justin D
JD
Besant
Adam
A
Mepham
Brenda
B
Green
Laili
L
Mahmoudian
Thaddeus
T
Gibbs
Ivaylo
I
Ivanov
Anahita
A
Malvea
Jessica
J
Stojcic
Alison L
AL
Allan
Lori E
LE
Lowes
Edward H
EH
Sargent
Robert K
RK
Nam
Shana O
SO
Kelley
D000951:Antigens, Neoplasm / Q000032:analysis; D014408:Biomarkers, Tumor / Q000032:analysis; D015815:Cell Adhesion Molecules / Q000032:analysis; D045744:Cell Line, Tumor; D002469:Cell Separation / Q000295:instrumentation*; D000071858:Epithelial Cell Adhesion Molecule; D058750:Epithelial-Mesenchymal Transition; D004867:Equipment Design; D006801:Humans; D058185:Magnetite Nanoparticles; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D009369:Neoplasms / Q000473:pathology*; D009360:Neoplastic Cells, Circulating / Q000473:pathology*
D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; D015815:Cell Adhesion Molecules; D000071858:Epithelial Cell Adhesion Molecule; D058185:Magnetite Nanoparticles
cancer cell analysis; cell sorting; magnetic nanoparticles; microfluidics; tumor cells