Multifunctional PEG retinylamine conjugate provides prolonged protection against retinal degeneration in mice.
A polyethylene glycol (PEG) retinylamine (Ret-NH2) conjugate PEG-GFL-NH-Ret with a glycine-phenylalanine-leucine (GFL) spacer was synthesized for controlled oral delivery of Ret-NH2 to treat retinal degenerative diseases, including Stargardt disease (STGD) and age-related macular degeneration (AMD). The peptide spacer was introduced for sustained release of the drug by digestive enzymes in the gastrointestinal tract. The pharmacokinetics experiments showed that the PEG conjugate could control the sustained drug release after oral administration and had much lower nonspecific liver drug accumulation than the free drug in wild-type female C57BL mice. In the mean time, the conjugate maintained the same concentration of Ret-NH2 in the eye as the free drug. Also, PEG-GFL-NH-Ret at a Ret-NH2 equivalent dose of 25 mg/kg produced complete protection of Abca4(-/-)Rdh8(-/-) mouse retinas against light-induced retinal degeneration for 3 days after oral administration, as revealed by OCT retina imaging, whereas free Ret-NH2 did not provide any protection under identical conditions. The polymer conjugate PEG-GFL-NH-Ret has great potential for controlled delivery of Ret-NH2 to the eye for effective protection against retinal degenerative diseases.
Department of Biomedical Engineering, School of Engineering, §Department of Pharmacology, Cleveland Center for Membrane and Structural Biology, School of Medicine, and ∥Department of Ophthalmology, School of Medicine, Case Western Reserve University , Cleveland, Ohio 44140, United States.
Guanping
G
Yu
Xueming
X
Wu
Nadia
N
Ayat
Akiko
A
Maeda
Song-Qi
SQ
Gao
Marcin
M
Golczak
Krzysztof
K
Palczewski
Zheng-Rong
ZR
Lu
D000818:Animals; D004224:Diterpenes / Q000737:chemistry* / Q000493:pharmacokinetics; D016503:Drug Delivery Systems; D005260:Female; D008027:Light / Q000009:adverse effects; D008268:Macular Degeneration / Q000151:congenital / Q000188:drug therapy / Q000473:pathology; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D011092:Polyethylene Glycols / Q000737:chemistry* / Q000493:pharmacokinetics; D012162:Retinal Degeneration / Q000188:drug therapy* / Q000473:pathology; D000080362:Stargardt Disease
D004224:Diterpenes; D011092:Polyethylene Glycols; C009167:retinylamine