Effects on growth of human osteoblast-like cells of three nonsteroidal anti-inflammatory drugs: metamizole, dexketoprofen, and ketorolac.
Some nonsteroidal anti-inflammatory drugs (NSAIDs) have adverse effects on bone tissue. The objective of this study was to determine the effect of different doses of dexketoprofen, ketorolac, and metamizole on growth of the osteoblast MG63 cell line. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide spectrophotometry results showed that MG63 cell growth was significantly inhibited after 24 hr of culture with doses of 10, 20, 100, or 1,000 µM of each NSAID and with doses of 0.1, 1, or 5 µM of dexketoprofen and ketorolac but not metamizole. Cell-cycle studies revealed that dexketoprofen and ketorolac treatments significantly arrested the cell cycle in phase G0/G1, increasing the percentage of cells in this phase. Apoptosis/necrosis studies showed significant changes versus control cells, with an increased percentage of cells in apoptosis after treatment with 10, 100, or 1,000 µM of metamizole and after treatment with 1, 10, 100, or 1,000 µM of dexketoprofen or ketorolac. In conclusion, treatment of osteoblast-like cells with high doses of the NSAIDs tested increased not only the percentage of cells in apoptosis but also the percentage of necrotic cells.
Department of Nursing, School of Health Sciences, University of Granada, Granada, Spain.
Elvira
E
De Luna-Bertos
Department of Nursing, School of Health Sciences, University of Granada, Granada, Spain.
Javier
J
Ramos-Torrecillas
Department of Nursing, School of Health Sciences, University of Granada, Granada, Spain.
Francisco Javier
FJ
Manzano-Moreno
Department of Nursing, School of Health Sciences, University of Granada, Granada, Spain.
Olga
O
García-Martínez
Department of Nursing, School of Health Sciences, Institute of Neurosciences, University of Granada, Granada crr@ugr.es.
Concepción
C
Ruiz
D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology; D002453:Cell Cycle / Q000187:drug effects; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects*; D004177:Dipyrone / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D006801:Humans; D007660:Ketoprofen / Q000494:pharmacology*; D020910:Ketorolac / Q000494:pharmacology*; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects*
D000894:Anti-Inflammatory Agents, Non-Steroidal; D004177:Dipyrone; D007660:Ketoprofen; D020910:Ketorolac
MG63; NSAIDs; bone tissue; cell growth; osteoblasts