Relationship between cotinine and trans-3'-hydroxycotinine glucuronidation and the nicotine metabolite ratio in Caucasian smokers.
BACKGROUND
Nicotine metabolite ratio (NMR) is linked with smoking behaviour and quitting, but little is known about the importance of UDP-glucuronosyltransferase metabolism phenotype on NMR.


OBJECTIVE
To investigate the relationship between NMR and UDP-glucuronosyltransferase phenotype in a Caucasian population.


METHODS
Urine samples from 260 smokers were measured by UPLC-MS/MS for cotinine (COT), 3'-hydroxycotinine, COT-glucuronide and 3'-hydroxycotinine-glucuronide. UDP-glucuronosyltransferase phenotype was assessed using the proportion of metabolites present as the glucuronide.


RESULTS
A correlation was found between NMR and UDP-glucuronosyltransferase phenotype for COT (males and females) and 3'-HC in females (p < 0.0001).


CONCLUSIONS
NMR is clearly influenced by UDP-glucuronosyltransferase phenotype for COT in Caucasians.
School of Medicine, University of Tasmania , Hobart , Australia.
Glenn A
GA
Jacobson
Stuart G
SG
Ferguson
D002853:Chromatography, Liquid; D003367:Cotinine / Q000031:analogs & derivatives* / Q000378:metabolism*; D005260:Female; D020719:Glucuronides / Q000378:metabolism*; D014453:Glucuronosyltransferase / Q000378:metabolism; D006801:Humans; D008297:Male; D009538:Nicotine / Q000378:metabolism*; D053719:Tandem Mass Spectrometry; D044465:White People
D020719:Glucuronides; C001381:hydroxycotinine; D009538:Nicotine; D014453:Glucuronosyltransferase; D003367:Cotinine
Genotype; nicotine metabolite ratio; smoking cessation