Vitamin B6 status, immune response and inflammation markers in kidney transplant recipients treated with polyclonal anti-thymocyte globulin.
OBJECTIVE
Vitamin B6 status has an impact on the body's inflammatory and immune responses. Immunosuppressive therapy may influence vitamin B6 metabolism in kidney transplant recipients. Treatment with polyclonal anti-thymocyte globulin (ATG) is associated with long-term changes in inflammatory and immune parameters. It is not known if ATG therapy also may have an impact on vitamin B6 status in kidney transplant recipients. We aimed to analyze the impact of therapy with ATG on vitamin B6 status, immune response, and the profile of inflammatory cytokines.


METHODS
This was a retrospective, observational study that included 44 kidney allograft recipients. Twenty patients received induction therapy with ATG (6 to 24 months before enrollment). Twenty-four patients constituted the control group, matched with respect to time since transplantation. The B6 vitamers, total lymphocyte count, CD3 percentage, interleukin (IL)-6, -7, and -10, transforming growth factor β, interferon γ, and chemokine ligand 21 were analyzed in a study group.


RESULTS
All indicators of vitamin B6 status were lower in the ATG group than in the control group. There were also significant differences with respect to immune response (significantly lower total lymphocyte count and CD3 in the ATG group) and inflammatory status (significantly higher IL-6 and IL-10 in the ATG group). Vitamin B6 vitamers and derivatives were not related to lymphocyte count and cytokine levels or to estimated glomerular filtration rate and age of the study population.


CONCLUSIONS
Vitamin B6 stores and active forms are lower in kidney transplant recipients treated with ATG. ATG therapy promotes CD3 and total lymphocyte depletion and increases indicators of inflammation. We found no associations between vitamers of B6, immune response cells, and inflammatory cytokines in study population.
Department of Nephrology, Transplantology and Internal Medicine, Medical University of Gdańsk, Poland. Electronic address: maja@gumed.edu.pl.
M
M
Jankowska
Department of Clinical Immunology and Transplantology, Medical University of Gdańsk, Poland.
P
P
Trzonkowski
Department of Nephrology, Transplantology and Internal Medicine, Medical University of Gdańsk, Poland.
A
A
Dębska-Ślizień
Department of Toxicology, Medical University of Gdańsk, Poland.
M
M
Marszałł
Department of Nephrology, Transplantology and Internal Medicine, Medical University of Gdańsk, Poland.
B
B
Rutkowski
D000328:Adult; D000961:Antilymphocyte Serum / Q000494:pharmacology; D016207:Cytokines / Q000097:blood; D005260:Female; D006084:Graft Rejection / Q000276:immunology; D006085:Graft Survival / Q000187:drug effects; D006801:Humans; D016753:Interleukin-10 / Q000097:blood; D015850:Interleukin-6 / Q000097:blood; D016030:Kidney Transplantation; D008297:Male; D008875:Middle Aged; D012372:ROC Curve; D012189:Retrospective Studies; D013601:T-Lymphocytes / Q000276:immunology*; D066027:Transplant Recipients; D025101:Vitamin B 6 / Q000097:blood*
D000961:Antilymphocyte Serum; D016207:Cytokines; D015850:Interleukin-6; D016753:Interleukin-10; D025101:Vitamin B 6