Development and validation of a liquid chromatography with mass spectrometry method to determine resveratrol and piceid isomers in beeswax.
This paper represents the first report of a liquid chromatography coupled to electrospray ionization mass spectrometry method for simultaneously analyzing resveratrol and piceid isomers (cis and trans) in beeswax. An efficient extraction procedure has been proposed (average analyte recoveries were between 89 and 95%); this involved a solid-liquid extraction using a mixture of ethanol and water (80:20, v/v) and a concentration step in a rotary evaporator. The separation of all the compounds was achieved using a C18 column and a mobile phase composed of ammonium formate 0.03 M in water and acetonitrile in gradient elution mode at a flow rate of 1 mL/min. The method was fully validated in terms of selectivity, limits of detection and quantification, linearity, precision, and accuracy. The limits of detection and quantification ranged from 1.0 to 1.7 and 3.5 to 5.5 μg/kg, respectively. Finally, the proposed method was applied to analyze beeswax samples collected from experimental and organic apiaries.
I.U. CINQUIMA, Analytical Chemistry Group, University of Valladolid, Valladolid, Spain.
Ana M
AM
Ares
Yolanda
Y
González
María J
MJ
Nozal
José L
JL
Bernal
Mariano
M
Higes
José
J
Bernal
D002853:Chromatography, Liquid / Q000379:methods*; D005960:Glucosides / Q000032:analysis*; D007536:Isomerism; D057230:Limit of Detection; D013058:Mass Spectrometry / Q000379:methods*; D012015:Reference Standards; D015203:Reproducibility of Results; D000077185:Resveratrol; D013267:Stilbenes / Q000032:analysis*; D014885:Waxes / Q000737:chemistry*
D005960:Glucosides; D013267:Stilbenes; D014885:Waxes; C038228:beeswax; D000077185:Resveratrol; C058229:polydatin
Beeswax; Liquid chromatography; Mass spectrometry; Piceid isomers; Resveratrol isomers