Bacterial endophytic communities in the grapevine depend on pest management.
Microbial plant endophytes are receiving ever-increasing attention as a result of compelling evidence regarding functional interaction with the host plant. Microbial communities in plants were recently reported to be influenced by numerous environmental and anthropogenic factors, including soil and pest management. In this study we used automated ribosomal intergenic spacer analysis (ARISA) fingerprinting and pyrosequencing of 16S rDNA to assess the effect of organic production and integrated pest management (IPM) on bacterial endophytic communities in two widespread grapevines cultivars (Merlot and Chardonnay). High levels of the dominant Ralstonia, Burkholderia and Pseudomonas genera were detected in all the samples We found differences in the composition of endophytic communities in grapevines cultivated using organic production and IPM. Operational taxonomic units (OTUs) assigned to the Mesorhizobium, Caulobacter and Staphylococcus genera were relatively more abundant in plants from organic vineyards, while Ralstonia, Burkholderia and Stenotrophomonas were more abundant in grapevines from IPM vineyards. Minor differences in bacterial endophytic communities were also found in the grapevines of the two cultivars.
Research and Innovation Centre, Fondazione Edmund Mach (FEM), S. Michele all'Adige (TN), Italy.
Andrea
A
Campisano
Research and Innovation Centre, Fondazione Edmund Mach (FEM), S. Michele all'Adige (TN), Italy; Austrian Institute of Technology GmbH, Department of Health & Environment, Bioresources Unit, Tulln, Austria.
Livio
L
Antonielli
Research and Innovation Centre, Fondazione Edmund Mach (FEM), S. Michele all'Adige (TN), Italy.
Michael
M
Pancher
Research and Innovation Centre, Fondazione Edmund Mach (FEM), S. Michele all'Adige (TN), Italy; Department of Environmental Sciences, Quaid-i-Azam University, Islamabad, Pakistan.
Sohail
S
Yousaf
Research and Innovation Centre, Fondazione Edmund Mach (FEM), S. Michele all'Adige (TN), Italy.
Massimo
M
Pindo
Research and Innovation Centre, Fondazione Edmund Mach (FEM), S. Michele all'Adige (TN), Italy.
Ilaria
I
Pertot
D001419:Bacteria / Q000235:genetics*; D019117:Burkholderia / Q000235:genetics; D004275:DNA, Ribosomal; D060026:Endophytes / Q000502:physiology*; D007558:Italy; D059013:Microbial Consortia / Q000502:physiology*; D010571:Pest Control; D011549:Pseudomonas / Q000235:genetics; D043367:Ralstonia / Q000235:genetics; D017422:Sequence Analysis, DNA / Q000379:methods; D027843:Vitis / Q000382:microbiology*
D004275:DNA, Ribosomal