Assessment of residual bio-efficacy and persistence of Ipomoea cairica plant extract against Culex quinquefasciatus Say mosquito.
Specification on residual action of a possible alternative insecticide derived from plant materials is important to determine minimum interval time between applications and the environmental persistence of the biopesticides. The objective of this study is to evaluate crude acethonilic extract of Ipomoea cairica leaves for its residual and persistence effects against Culex quinquefasciatus larvae. Wild strain of Cx. quinquefasciatus larvae were used for the purpose of the study. Two test designs, replenishment of water and without replenishment of water were carried out. For the first design, a total of 10 ml of test solution containing Ip. cairica extracts was replenished daily and replaced with 10 ml of distilled water. For the second design, treatment water was maintained at 1500 ml and only evaporated water was refilled. Larval mortality was recorded at 24 hours post-treatment after each introduction period and trials were terminated when mortality rate falls below 50%. Adult emergences from survived larvae were observed and number of survivals was recorded. For the non-replenishment design, mortality rate significantly reduced to below 50% after 28 days, meanwhile for replenishment of water declined significantly after 21 days (P < 0.05). There was no adult emergence observed up to seven days for non-replenishment and first two days for replenishment of water design. The short period of residual effectiveness of crude acethonilic extract of Ip. cairica leaves with high percentage of larval mortality on the first few days, endorses fewer concerns of having excess residues in the environment which may carry the risk of insecticide resistance and environmental pollution.
School of Biological Sciences, Universiti Sains Malaysia, Penang 11800, Malaysia.
Maniam
M
Thiagaletchumi
School of Biological Sciences, Universiti Sains Malaysia, Penang 11800, Malaysia.
Wan Fatma
WF
Zuharah
School of Biological Sciences, Universiti Sains Malaysia, Penang 11800, Malaysia.
Rattanam
R
Ahbi  Rami
School of Biological Sciences, Universiti Sains Malaysia, Penang 11800, Malaysia.
Nik
N
Fadzly
School of Biological Sciences, Universiti Sains Malaysia, Penang 11800, Malaysia.
Hamady
H
Dieng
School of Biological Sciences, Universiti Sains Malaysia, Penang 11800, Malaysia.
Abu Hassan
AH
Ahmad
Department of Medical Microbiology, University of Malaya, Kuala Lumpur 50603, Malaysia.
Sazaly
S
AbuBakar
D000818:Animals; D001681:Biological Assay; D003465:Culex / Q000187:drug effects*; D004355:Drug Stability; D007306:Insecticides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D027741:Ipomoea / Q000737:chemistry*; D007814:Larva / Q000187:drug effects; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry; D016019:Survival Analysis
D007306:Insecticides; D010936:Plant Extracts