Preliminary characterization of a cholinesterase from roots of Bengal gram - Cicer arietinum L.
A Cholinesterase was isolated and purified (65-fold) from roots of Bengal gram (Cicer arietinum L.) seedlings. It hydrolyzes acetylcholine and acetylthiocholine more readily than propionylthiocholine or butyrylthiocholine. The enzyme has high affinity for acetylthiocholine (Km=150 μm) and is inhibited by animal anticholinesterases, neostigmine and physostigmine, and by Phosfon-D, a plant growth retardant.
Department of Botany, University of Delhi, Delhi-110007, India.
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R
Gupta
Department of Botany, University of Delhi, Delhi-110007, India.
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SC
Maheshwari
Acetylcholine; Bengal gram; Cholinesterase; Cicer arietinum; Neostigmine; Phosfon-D; Physostigmine