Improving enzymatic hydrolysis of corn stover pretreated by ethylene glycol-perchloric acid-water mixture.
To improve the enzymatic saccharification of lignocellulosic biomass, a mixture of ethylene glycol-HClO4-water (88.8:1.2:10, w/w/w) was used for pretreating corn stover in this study. After the optimization in oil-bath system, the optimum pretreatment temperature and time were 130 °C and 30 min, respectively. After the saccharification of 10 g/L pretreated corn stover for 48 h, the saccharification rate was obtained in the yield of 77.4 %. To decrease pretreatment temperature and shorten pretreatment time, ethylene glycol-HClO4-water (88.8:1.2:10, w/w/w) media under microwave irradiation was employed to pretreat corn stover effectively at 100 °C and 200 W for 5 min. Finally, the recovered hydrolyzates containing glucose obtained from the enzymatic hydrolysis of pretreated corn stovers could be fermented into ethanol efficiently. These results would be helpful for developing a cost-effective pretreatment combined with enzymatic saccharification of cellulosic materials for the production of lignocellulosic ethanol.
Laboratory of Biochemical Engineering, College of Pharmaceutical Engineering and Life Sciences, Changzhou University, Changzhou, 213164, China, heyucai2001@163.com.
Yu-Cai
YC
He
Feng
F
Liu
Lei
L
Gong
Ting
T
Lu
Yun
Y
Ding
Dan-Ping
DP
Zhang
Qing
Q
Qing
Yue
Y
Zhang
D000143:Acids / Q000494:pharmacology; D001709:Biotechnology / Q000379:methods*; D050260:Carbohydrate Metabolism / Q000187:drug effects; D002480:Cellulase / Q000378:metabolism*; D019855:Ethylene Glycol / Q000494:pharmacology*; D005285:Fermentation / Q000187:drug effects; D006868:Hydrolysis; D008872:Microwaves; D009821:Oils; D010472:Perchlorates / Q000494:pharmacology*; D017550:Spectroscopy, Fourier Transform Infrared; D014866:Waste Products; D014867:Water / Q000494:pharmacology*; D003313:Zea mays / Q000737:chemistry* / Q000187:drug effects / Q000648:ultrastructure
D000143:Acids; D009821:Oils; D010472:Perchlorates; D014866:Waste Products; D014867:Water; D002480:Cellulase; D019855:Ethylene Glycol