Children living near a sanitary landfill have increased breath methane and Methanobrevibacter smithii in their intestinal microbiota.
This study evaluated the breath CH4 excretion and concentration of M. smithii in intestinal microbiota of schoolchildren from 2 slums. One hundred and eleven children from a slum near a sanitary landfill, 35 children of a slum located away from the sanitary landfill, and 32 children from a high socioeconomic level school were included in the study. Real-time PCR was performed to quantify the M. smithii nifH gene and it was present in the microbiota of all the participating children, with higher (P < 0.05) concentrations in those who lived in the slum near the landfill (3.16 × 10(7) CFU/g of feces), comparing with the children from the slum away from the landfill (2.05 × 10(6) CFU/g of feces) and those from the high socioeconomic level group (3.93 × 10(5) CFU/g of feces). The prevalence of children who present breath methane was 53% in the slum near the landfill, 31% in the slum further away from the landfill and, 22% in the high socioeconomic level group. To live near a landfill is associated with higher concentrations of M. smithii in intestinal microbiota, comparing with those who live away from the landfill, regardless of their socioeconomics conditions.
Division of Pediatric Gastroenterology, Universidade Federal de São Paulo, 598 Botucatu Street, Vila Clementino, 04023-062 São Paulo, SP, Brazil ; Centro Universitário FIEO, 300 Franz Voegeli Avenida, Vila Yara, 06020-190 Osasco, SP, Brazil.
Humberto Bezerra
0000-0001-6438-0995
HB
de Araujo Filho
Centro Universitário FIEO, 300 Franz Voegeli Avenida, Vila Yara, 06020-190 Osasco, SP, Brazil.
Mirian Silva
MS
Carmo-Rodrigues
Division of Pediatric Gastroenterology, Universidade Federal de São Paulo, 598 Botucatu Street, Vila Clementino, 04023-062 São Paulo, SP, Brazil.
Carolina Santos
CS
Mello
Division of Pediatric Gastroenterology, Universidade Federal de São Paulo, 598 Botucatu Street, Vila Clementino, 04023-062 São Paulo, SP, Brazil ; Centro Universitário FIEO, 300 Franz Voegeli Avenida, Vila Yara, 06020-190 Osasco, SP, Brazil.
Lígia Cristina Fonseca Lahoz
LC
Melli
Division of Pediatric Gastroenterology, Universidade Federal de São Paulo, 598 Botucatu Street, Vila Clementino, 04023-062 São Paulo, SP, Brazil.
Soraia
S
Tahan
Division of Medicine, Universidade Federal de São Paulo, 188 Leandro Dupret Street, Vila Clementino, 04025-010 São Paulo, SP, Brazil.
Antonio Carlos Campos
AC
Pignatari
Division of Pediatric Gastroenterology, Universidade Federal de São Paulo, 598 Botucatu Street, Vila Clementino, 04023-062 São Paulo, SP, Brazil.
Mauro Batista
MB
de Morais
D001938:Brazil; D001944:Breath Tests; D002648:Child; D041981:Gastrointestinal Tract / Q000382:microbiology*; D006801:Humans; D008697:Methane / Q000032:analysis*; D041581:Methanobrevibacter / Q000302:isolation & purification*; D010088:Oxidoreductases / Q000235:genetics; D011204:Poverty Areas; D060888:Real-Time Polymerase Chain Reaction; D013334:Students; D064091:Waste Disposal Facilities
D010088:Oxidoreductases; C029130:nitrogenase reductase; D008697:Methane