Computational evidence for structural consequences of kiteplatin damage on DNA.
The reaction of the potential anticancer drug kiteplatin, cis-[PtCl2(cis-1,4-DACH)], with oligomers of single- and double-stranded DNA ranging from 2 to 12 base pairs in length was performed as a model for DNA interaction. The potential for conformational flexibility of single-stranded adducts was examined with density functional theory (DFT) and compared with data from (1)H-NMR 1D and 2D spectroscopy. This indicates the presence of multiple conformations of an adduct with d(GpG), but only one form of the adduct with d(TGGT). The importance of a suitable theoretical model, and in particular basis set, in reproducing experimental data is demonstrated. The DFT theoretical model was extended to platinated base pair step (GG/CC), allowing a comparison to the related compounds cisplatin and oxaliplatin. Adducts of kiteplatin with larger fragments of double-stranded DNA, including tetramer, octamer, and dodecamer, were studied theoretically using hybrid quantum mechanics/molecular mechanics methods. Structural parameters of all the base-paired models were evaluated and binding energies calculated in gas phase and in solution; these are compared across the series and also with the related complexes cisplatin and oxaliplatin, thus revealing insights into how kiteplatin binds to DNA and similarities and differences between this and related compounds.
School of Chemistry, Cardiff University, Park Place, Cardiff, CF10 3AT, UK.
Shaun T
ST
Mutter
Department of Chemistry, University of Bari Aldo Moro, Via E. Orabona 4, 70125, Bari, Italy.
Nicola
N
Margiotta
Department of Biotechnology and Environmental Sciences, University of Salento, via Monteroni, 73100, Lecce, Italy.
Paride
P
Papadia
School of Chemistry, Cardiff University, Park Place, Cardiff, CF10 3AT, UK. platts@cf.ac.uk.
James A
JA
Platts
D000970:Antineoplastic Agents / Q000737:chemistry*; D020029:Base Pairing; D003198:Computer Simulation; D004249:DNA Damage; D004277:DNA, Single-Stranded / Q000737:chemistry*; D008958:Models, Molecular; D009944:Organoplatinum Compounds / Q000737:chemistry*
D000970:Antineoplastic Agents; D004277:DNA, Single-Stranded; D009944:Organoplatinum Compounds; C445102:platinum(II)(1,4-diazacycloheptane)dichloride