The low level laser therapy (LLLT) operating in 660 nm reduce gene expression of inflammatory mediators in the experimental model of collagenase-induced rat tendinitis.
Tendinopathy is a common disease with a variety of treatments and therapies. Laser therapy appears as an alternative treatment. Here, we investigate the effects of laser irradiation in an experimental model of tendinitis induced by collagenase injection on rats' Achilles tendon, verifying its action in important inflammatory markers. Male Wistar rats were used and divided into five groups: control saline (C), non-treated tendinitis (NT) and tendinitis treated with sodium diclofenac (D) or laser (1 J) and (3 J). The tendinitis was induced by collagenase (100 μg/tendon) on the Achilles tendon, which was removed for further analyses. The gene expression for COX-2; TNF-α; IL-6; and IL-10 (RT-PCR) was measured. The laser irradiation (660 nm, 100 mW, 3 J) used in the treatment of the tendinitis induced by collagenase in Achilles tendon in rats was effective in the reduction of important pro-inflammatory markers such as IL-6 and TNF-α, becoming a promising tool for the treatment of tendon diseases.
Postgraduate Program in Biophotonics Applied to Health Sciences, Nove de Julho University, São Paulo, 01504-001, Brazil.
Romildo
R
Torres-Silva
Rodrigo Alvaro Brandão
RA
Lopes-Martins
Jan Magnus
JM
Bjordal
Lucio
L
Frigo
Rachid
R
Rahouadj
Gilles
G
Arnold
Ernesto Cesar Pinto
EC
Leal-Junior
Jacques
J
Magdalou
Rodney
R
Pallotta
Rodrigo Labat
RL
Marcos
D000125:Achilles Tendon / Q000378:metabolism / Q000473:pathology / Q000528:radiation effects*; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000627:therapeutic use; D017364:Collagenases; D051546:Cyclooxygenase 2 / Q000235:genetics / Q000378:metabolism; D004008:Diclofenac / Q000627:therapeutic use; D004195:Disease Models, Animal; D015870:Gene Expression / Q000528:radiation effects*; D016753:Interleukin-10 / Q000235:genetics / Q000378:metabolism; D015850:Interleukin-6 / Q000235:genetics / Q000378:metabolism; D028022:Low-Level Light Therapy; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D052256:Tendinopathy / Q000139:chemically induced / Q000378:metabolism / Q000532:radiotherapy*; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000378:metabolism
D000894:Anti-Inflammatory Agents, Non-Steroidal; D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10; D004008:Diclofenac; D051546:Cyclooxygenase 2; C489691:Ptgs2 protein, rat; D017364:Collagenases