Heterogeneous nuclear ribonucleoprotein E1 regulates protein disulphide isomerase translation in oxidized low-density lipoprotein-activated endothelial cells.
OBJECTIVE
Endothelium-derived protein disulphide isomerase (PDI) is required for thrombus formation in vivo. But, how to control PDI overproduction in oxidized low-density lipoprotein (oxLDL)-activated vascular endothelial cells (VECs) is not well understood. In this study, we try to answer this question using our newly identified activator of mTOC1 3-benzyl-5-((2-nitrophenoxy) methyl)-dihydrofuran-2 (3H)-one (3BDO) that has been shown to protect VECs.


METHODS
First, we performed a proteomics analysis on the oxLDL-activated vascular VECs in the presence or absence of 3BDO. Next, we constructed the heterogeneous nuclear ribonucleoprotein E1 (hnRNP E1) mutants at Ser43 and used the RNA-ChIP technique to investigate the relationship between hnRNP E1 and PDI production. Furthermore, we examined the effect of 3BDO on oxLDL-altered phosphorylation of Akt1 and Akt2. Finally, we studied the effect of 3BDO on oxLDL-altered PDI protein level in apolipoprotein E(-/-) mice with advanced atherosclerosis.


RESULTS
In VECs, oxLDL-increased PDI protein level, induced hnRNP E1 phosphorylation at Ser43, suppressed the binding of hnRNP E1 to PDI 5'UTR and induced the phosphorylation of Akt2 but not Akt1. All of these processes were blocked by 3BDO. Importantly, Ser43 mutant of hnRNP E1 inhibited the increase of PDI protein level and the decrease of the binding of hnRNP E1 and PDI 5'UTR induced by oxLDL. Furthermore, 3BDO suppressed oxLDL-induced PDI protein increase in the serum and plaque endothelium of apolipoprotein E(-/-) mice.


CONCLUSIONS
hnRNP E1 is a new regulator of PDI translation in oxLDL-activated VECs, and 3BDO is a powerful agent for controlling PDI overproduction.
Shandong Provincial Key Laboratory of Animal Cells and Developmental Biology, School of Life Science, Shandong University, Jinan, China; School of Biological Science and Technology, University of Jinan, Jinan, China.
N
N
Meng
N
N
Peng
S
S
Huang
S Q
SQ
Wang
J
J
Zhao
L
L
Su
Y
Y
Zhang
S
S
Zhang
B
B
Zhao
J
J
Miao
D015107:4-Butyrolactone / Q000031:analogs & derivatives / Q000494:pharmacology; D020121:5' Untranslated Regions; D000818:Animals; D001057:Apolipoproteins E / Q000172:deficiency / Q000235:genetics; D050197:Atherosclerosis / Q000201:enzymology* / Q000235:genetics; D001665:Binding Sites; D002478:Cells, Cultured; D004268:DNA-Binding Proteins; D004195:Disease Models, Animal; D034441:Heterogeneous-Nuclear Ribonucleoproteins / Q000235:genetics / Q000378:metabolism*; D061307:Human Umbilical Vein Endothelial Cells / Q000187:drug effects / Q000201:enzymology*; D006801:Humans; D008077:Lipoproteins, LDL / Q000378:metabolism*; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009154:Mutation; D010766:Phosphorylation; D019704:Protein Disulfide-Isomerases / Q000096:biosynthesis* / Q000235:genetics; D040901:Proteomics / Q000379:methods; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D016601:RNA-Binding Proteins; D014162:Transfection
C553471:3-benzyl-5-((2-nitrophenoxy)methyl)dihydrofuran-2(3H)-one; D020121:5' Untranslated Regions; D001057:Apolipoproteins E; D004268:DNA-Binding Proteins; D034441:Heterogeneous-Nuclear Ribonucleoproteins; D008077:Lipoproteins, LDL; C094604:PCBP1 protein, human; D016601:RNA-Binding Proteins; C099252:oxidized low density lipoprotein; C494921:AKT2 protein, human; D051057:Proto-Oncogene Proteins c-akt; D019704:Protein Disulfide-Isomerases; D015107:4-Butyrolactone
heterogeneous nuclear ribonucleoprotein E1; protein disulphide isomerase; vascular endothelial cells