Structure of the OsSERK2 leucine-rich repeat extracellular domain.
Somatic embryogenesis receptor kinases (SERKs) are leucine-rich repeat (LRR)-containing integral membrane receptors that are involved in the regulation of development and immune responses in plants. It has recently been shown that rice SERK2 (OsSERK2) is essential for XA21-mediated resistance to the pathogen Xanthomonas oryzae pv. oryzae. OsSERK2 is also required for the BRI1-mediated, FLS2-mediated and EFR-mediated responses to brassinosteroids, flagellin and elongation factor Tu (EF-Tu), respectively. Here, crystal structures of the LRR domains of OsSERK2 and a D128N OsSERK2 mutant, expressed as hagfish variable lymphocyte receptor (VLR) fusions, are reported. These structures suggest that the aspartate mutation does not generate any significant conformational change in the protein, but instead leads to an altered interaction with partner receptors.
Physical Biosciences Division, Lawrence Berkeley National Laboratory, 1 Cyclotron Road, Berkeley, CA 94720, USA.
Ryan
R
McAndrew
Plant Pathology Faculty, The Genome Center, University of California, Davis, CA 95616, USA.
Rory N
RN
Pruitt
Department of Entomology, University of California, Davis, CA 95616, USA.
Shizuo G
SG
Kamita
Physical Biosciences Division, Lawrence Berkeley National Laboratory, 1 Cyclotron Road, Berkeley, CA 94720, USA.
Jose Henrique
JH
Pereira
Plant Pathology Faculty, The Genome Center, University of California, Davis, CA 95616, USA.
Dipali
D
Majumdar
Department of Entomology, University of California, Davis, CA 95616, USA.
Bruce D
BD
Hammock
Physical Biosciences Division, Lawrence Berkeley National Laboratory, 1 Cyclotron Road, Berkeley, CA 94720, USA.
Paul D
PD
Adams
Plant Pathology Faculty, The Genome Center, University of California, Davis, CA 95616, USA.
Pamela C
PC
Ronald
D000595:Amino Acid Sequence; D000818:Animals; D002460:Cell Line; D018360:Crystallography, X-Ray; D016350:Leucine Zippers; D008958:Models, Molecular; D008969:Molecular Sequence Data; D012275:Oryza / Q000737:chemistry* / Q000235:genetics; D010940:Plant Proteins / Q000737:chemistry* / Q000235:genetics; D017354:Point Mutation; D011487:Protein Conformation; D011494:Protein Kinases / Q000737:chemistry* / Q000235:genetics; D017434:Protein Structure, Tertiary; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics
D010940:Plant Proteins; D011993:Recombinant Fusion Proteins; D011494:Protein Kinases; C106294:SERK protein, plant
OsSERK2; SERKs; leucine-rich repeats