Morphological phenotyping of mouse hearts using optical coherence tomography.
Transgenic mouse models have been instrumental in the elucidation of the molecular mechanisms behind many genetically based cardiovascular diseases such as Marfan syndrome (MFS). However, the characterization of their cardiac morphology has been hampered by the small size of the mouse heart. In this report, we adapted optical coherence tomography (OCT) for imaging fixed adult mouse hearts, and applied tools from computational anatomy to perform morphometric analyses. The hearts were first optically cleared and imaged from multiple perspectives. The acquired volumes were then corrected for refractive distortions, and registered and stitched together to form a single, high-resolution OCT volume of the whole heart. From this volume, various structures such as the valves and myofibril bundles were visualized. The volumetric nature of our dataset also allowed parameters such as wall thickness, ventricular wall masses, and luminal volumes to be extracted. Finally, we applied the entire acquisition and processing pipeline in a preliminary study comparing the cardiac morphology of wild-type mice and a transgenic mouse model of MFS.
Simon Fraser University, Faculty of Applied Science, School of Engineering Science, 8888 University Drive, Burnaby, BC V5A 1S6, Canada.
Michelle
M
Cua
Simon Fraser University, Faculty Science, Department of Biomedical Physiology and Kinesiology, 8888 University Drive, Burnaby, BC V5A 1S6, Canada.
Eric
E
Lin
Simon Fraser University, Faculty Science, Department of Biomedical Physiology and Kinesiology, 8888 University Drive, Burnaby, BC V5A 1S6, CanadacChild and Family Research Institute, Department of Cardiovascular Science, 950 West 28th Avenue, Vancouver, B.
Ling
L
Lee
Child and Family Research Institute, Department of Cardiovascular Science, 950 West 28th Avenue, Vancouver, BC V5Z 4H4, Canada.
Xiaoye
X
Sheng
Simon Fraser University, Faculty of Applied Science, School of Engineering Science, 8888 University Drive, Burnaby, BC V5A 1S6, Canada.
Kevin S K
KS
Wong
Simon Fraser University, Faculty Science, Department of Biomedical Physiology and Kinesiology, 8888 University Drive, Burnaby, BC V5A 1S6, CanadacChild and Family Research Institute, Department of Cardiovascular Science, 950 West 28th Avenue, Vancouver, B.
Glen F
GF
Tibbits
Simon Fraser University, Faculty of Applied Science, School of Engineering Science, 8888 University Drive, Burnaby, BC V5A 1S6, Canada.
Mirza Faisal
MF
Beg
Simon Fraser University, Faculty of Applied Science, School of Engineering Science, 8888 University Drive, Burnaby, BC V5A 1S6, Canada.
Marinko V
MV
Sarunic
D000818:Animals; D057791:Cardiac Imaging Techniques / Q000379:methods*; D004195:Disease Models, Animal; D005990:Glycerol; D006321:Heart / Q000033:anatomy & histology*; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D008382:Marfan Syndrome / Q000473:pathology; D051379:Mice; D008822:Mice, Transgenic; D009206:Myocardium / Q000473:pathology; D041623:Tomography, Optical Coherence / Q000379:methods*
D005990:Glycerol