Molecularly imprinted polymers based on SBA-15 for selective solid-phase extraction of baicalein from plasma samples.
Highly selective molecularly imprinted mesoporous silica polymer (SBA-15@MIP) for baicalein (BAI) extraction was synthesized using a surface molecular imprinting technique on the SBA-15 supporter. Computational simulation was used to predict the optimal functional monomer for the rational design of SBA-15@MIP. Meanwhile, high adsorption capacity was obtained when a suitable yield of molecularly imprinted polymers (MIPs) layer was grafted onto the surface of SBA-15. Characterization and performance tests of the obtained polymer revealed that SBA-15@MIP possessed a highly ordered mesoporous structure, reached saturated adsorption within 60 min, and exhibited higher sorption capacity to the target molecule BAI compared with non-imprinted mesoporous silica polymer (SBA-15@NIP) and SBA-15. Finally, SBA-15@MIP was successfully applied to solid-phase extraction (SPE) coupled with high-performance liquid chromatography and ultraviolet detection (HPLC-UV) for the determination of trace BAI in plasma samples. Mean recoveries of BAI through the molecularly imprinted solid-phase extraction (MISPE) sorbent, non-imprinted solid-phase extraction (NISPE) sorbent, and SBA-15 solid-phase extraction (SBA-15-SPE) sorbent were 94.4, 22.7, and 10.7 %, respectively, and the relative standard deviations were 2.9, 2.6, and 3.6 %, respectively. These results reveal that SBA-15@MIP as a SPE sorbent has good applicability to selectively separate and enrich trace BAI from complex samples.
School of Pharmacy, Nanjing Medical University, Nanjing, 210029, China.
Hongliang
H
He
Xiaoli
X
Gu
Liying
L
Shi
Junli
J
Hong
Hongjuan
H
Zhang
Yankun
Y
Gao
Shuhu
S
Du
Lina
L
Chen
D000327:Adsorption; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D044950:Flavanones / Q000097:blood* / Q000302:isolation & purification*; D006801:Humans; D008855:Microscopy, Electron, Scanning; D054802:Molecular Imprinting / Q000379:methods*; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry; D051381:Rats; D015203:Reproducibility of Results; D012822:Silicon Dioxide / Q000737:chemistry*; D052616:Solid Phase Extraction / Q000379:methods*; D017550:Spectroscopy, Fourier Transform Infrared; D013818:Thermogravimetry; D014466:Ultraviolet Rays
D044950:Flavanones; D011108:Polymers; C509969:SBA-15; C006680:baicalein; D012822:Silicon Dioxide