Evaluation of matrix-assisted laser desorption ionization-time of flight mass spectrometry for identification of Vibrio cholerae.
We evaluated the use of matrix-assisted laser desorption ionization-time of flight mass spectrometry (MS) for the identification of Vibrio cholerae. MS identified all 42 isolates of V. cholerae O1 and O139 and 7 of 9 non-O1/O139 isolates. MS correctly discriminated between all Aeromonas and V. cholerae isolates. Overall, MS performed as well as or better than biochemical methods.
Department of Pathology, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts, USA Department of Medicine, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts, USA Division of Infectious Diseases, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts, USA.
Jenna
J
Rychert
bioMérieux, Hazelwood, Missouri, USA.
David
D
Creely
Division of Infectious Diseases, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts, USA.
Leslie M
LM
Mayo-Smith
Department of Medicine, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts, USA Division of Infectious Diseases, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts, USA.
Stephen B
SB
Calderwood
Department of Global Health and Social Medicine, Harvard Medical School, Boston, Massachusetts, USA Partners In Health, Boston, Massachusetts, USA.
Louise C
LC
Ivers
Department of Medicine, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts, USA Division of Infectious Diseases, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts, USA.
Edward T
ET
Ryan
National Public Health Laboratory, Port-au-Prince, Haiti.
Jacques
J
Boncy
International Centre for Diarrhoeal Disease Research, Dhaka, Bangladesh.
Firdausi
F
Qadri
International Centre for Diarrhoeal Disease Research, Dhaka, Bangladesh.
Dilruba
D
Ahmed
Department of Pathology, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts, USA Department of Medicine, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts, USA.
Mary Jane
MJ
Ferraro
Department of Medicine, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts, USA Division of Infectious Diseases, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts, USA Department of Pediatrics, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts, USA jbharris@partners.org.
Jason B
JB
Harris
D001426:Bacterial Proteins / Q000235:genetics; D015373:Bacterial Typing Techniques / Q000379:methods*; D002771:Cholera / Q000175:diagnosis* / Q000382:microbiology; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D006801:Humans; D058889:Molecular Typing / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D014734:Vibrio cholerae / Q000737:chemistry* / Q000145:classification
D001426:Bacterial Proteins; D004269:DNA, Bacterial