Characterization and miRNA-mediated posttranscriptional regulation of vitelline membrane outer layer protein I in the adult chicken oviduct.
The laying hen is the best model for oviduct growth and development. The chicken oviduct produces the egg components, including the egg white and eggshell. However, the mechanism of egg component production during oviduct development requires further investigation. Vitelline membrane outer layer protein 1 (VMO-1) is found in the outer layer of the vitelline membrane of avian eggs. Comparison of the chicken VMO-1 protein-coding sequence and the human, mouse, rat, and bovine VMO-1 proteins via multiple sequence alignment analysis revealed high degrees of homology of 55%, 53%, 48%, and 54%, respectively. Although the avian homologue of VMO-1 is highly expressed in the magnum of the oviduct, little is known about the transcriptional and posttranscriptional regulation of VMO-1 during oviduct development. The results of this study revealed that estrogen induces VMO-1 messenger RNA (mRNA) expression in oviduct cells in vitro. The expression of genes interacting with VMO-1 by RNA interference (RNAi) functional analysis revealed that ovomucin expression was decreased by VMO-1 silencing. In addition, gga-miR-1623, 1552-3p, and 1651-3p influenced VMO-1 expression via its 3'-UTR, suggesting the posttranscriptional regulation of VMO-1 expression in chickens. Collectively, these results suggest that VMO-1 is an estrogen-induced gene that is posttranscriptionally regulated by microRNAs (miRNAs). The present study may contribute to an understanding of egg component production during chicken oviduct development.
Animal Biotechnology Division, Rural Development Administration, National Institute of Animal Science, Seosuwon-ro, Gwonseon-gu, Suwon, 441-706, Republic of Korea.
Sang
S
In Lee
Mi-Ran
MR
Ji
Ye-Jin
YJ
Jang
Mi-hyang
MH
Jeon
Jeom Sun
JS
Kim
Jin-Ki
JK
Park
Ik-Soo
IS
Jeon
Sung June
SJ
Byun
D000375:Aging / Q000235:genetics; D000595:Amino Acid Sequence; D000818:Animals; D030161:Avian Proteins / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D002645:Chickens / Q000235:genetics* / Q000254:growth & development*; D030561:Databases, Nucleic Acid; D004958:Estradiol / Q000494:pharmacology; D005260:Female; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000187:drug effects; D055785:Gene Knockdown Techniques; D016678:Genome; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D010057:Oviducts / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D010802:Phylogeny; D015203:Reproducibility of Results; D016415:Sequence Alignment; D020539:Sequence Analysis, Protein; D014158:Transcription, Genetic / Q000187:drug effects; D014817:Vitelline Membrane / Q000187:drug effects / Q000378:metabolism*
D030161:Avian Proteins; D035683:MicroRNAs; D004958:Estradiol