Effects of feeding polyphenol-rich winery wastes on digestibility, nitrogen utilization, ruminal fermentation, antioxidant status and oxidative stress in wethers.
Four wethers were used in a 4 × 4 Latin square design experiment to evaluate the availability of two types of winery wastes, winery sediment and grape pomace, as ruminant feeds possessing antioxidant activities. Each wether was assigned to one of the following four treatments: (i) 75 g/kg winery sediment (WS) on a dry matter (DM) basis; (ii) 166 g/kg DM winery grape pomace (WP); (iii) control diet (CD; 17 g/kg DM soybean meal);and (iv) only tall fescue hay (TFH; no additive). Winery sediment and grape pomace had high levels of polyphenols and of radical scavenging activities. Feeding with winery sediment and grape pomace did not negatively affect the intake, but it depressed crude protein (CP) digestibility compared with CD (P = 0.052 and P < 0.01 for WS and WP, respectively). Polyphenols in winery wastes decreased ruminal ammonia production (P = 0.089 and P < 0.05), likely due to their inhibitive effect on microbial activities in the rumen. The addition of winery sediment and grape pomace decreased urinary 8-hydroxy-2'-deoxyguanosine (8-OHdG; an index of oxidative damages) excretion per day (P < 0.05 and P = 0.059). The results indicated that winery sediment and grape pomace could alter nitrogen metabolism and/or act as new antioxidants for ruminants.
Laboratory of Animal Husbandry Resources, Division of Applied Biosciences, Graduate School of Agriculture, Kyoto University, Kyoto, Japan.
Kyohei
K
Ishida
Yosuke
Y
Kishi
Kazato
K
Oishi
Hiroyuki
H
Hirooka
Hajime
H
Kumagai
D000821:Animal Feed / Q000032:analysis; D000824:Animal Nutritional Physiological Phenomena / Q000502:physiology*; D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D004063:Digestion / Q000502:physiology*; D005285:Fermentation / Q000502:physiology*; D005525:Food-Processing Industry; D006801:Humans; D007220:Industrial Waste / Q000032:analysis; D008297:Male; D009584:Nitrogen / Q000378:metabolism*; D018384:Oxidative Stress / Q000502:physiology*; D059808:Polyphenols / Q000032:analysis; D012417:Rumen / Q000382:microbiology / Q000502:physiology*; D012756:Sheep / Q000378:metabolism* / Q000502:physiology*; D062611:Solid Waste / Q000032:analysis; D027843:Vitis / Q000737:chemistry; D014920:Wine
D000975:Antioxidants; D007220:Industrial Waste; D059808:Polyphenols; D062611:Solid Waste; D009584:Nitrogen
antioxidant; polyphenols; wethers; winery grape pomace; winery sedimant