Characterization of incomplete vitellogenin (VgC) in the Indian freshwater murrel, Channa punctatus (Bloch).
A novel incomplete vitellogenin (VgC) was purified from the plasma of estradiol-treated male murrel, Channa punctatus, by gel filtration chromatography. The native mass of VgC protein was 180 kDa, and it resolved as a single peptide of 100 kDa on SDS-PAGE. The peptide on subjecting to matrix-assisted laser desorption/ionization-time of flight produced a peptide mass fingerprint. On tandem mass spectrometry, some of these peptides showed mass to charge (m/z) ratio and amino acid sequence similarity with VgC peptides of other teleosts. Phylogenetic analysis revealed a similarity of murrel VgC with fish species of the order Perciformes. Semi-quantitative RT-PCR assay was developed to study expression of vgc gene at variable levels of estradiol exposure. Presence of VgC in males indicates that fish has been exposed to estrogens; hence, it can be used as a biomarker for estrogenic exposure.
Department of Zoology, University of Delhi, Delhi, 110007, India.
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Pipil
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Rawat
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Sharma
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Sehgal
D000595:Amino Acid Sequence; D000818:Animals; D015415:Biomarkers / Q000097:blood*; D002850:Chromatography, Gel / Q000662:veterinary; D004591:Electrophoresis, Polyacrylamide Gel / Q000662:veterinary; D004958:Estradiol / Q000494:pharmacology; D016013:Likelihood Functions; D008297:Male; D008957:Models, Genetic; D008969:Molecular Sequence Data; D010473:Perciformes / Q000235:genetics*; D010802:Phylogeny; D060888:Real-Time Polymerase Chain Reaction; D017385:Sequence Homology; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D053719:Tandem Mass Spectrometry; D014819:Vitellogenins / Q000097:blood / Q000235:genetics* / Q000302:isolation & purification
D015415:Biomarkers; D014819:Vitellogenins; D004958:Estradiol