Greek Pinus essential oils: larvicidal activity and repellency against Aedes albopictus (Diptera: Culicidae).
The needle volatiles metabolites of seven Pinus spp.: Pinus nigra (3 samples), Pinus stankewiczii, Pinus brutia, Pinus halepensis, Pinus canariensis, Pinus pinaster and Pinus strobus from Greece were determined by gas chromatography and gas chromatography-mass spectrometry. P. nigra and P. canariensis essential oils were dominated by α-pinene (24.9-28.9 % and 15 %, respectively) and germacrene D (20.3-31.9 % and 55.8 %, respectively), whereas P. brutia and P. strobus by α-pinene (20.6 % and 31.4 %, respectively) and β-pinene (31.7 % and 33.6 %, respectively). P. halepensis and P. pinaster oils were characterized by β-caryophyllene (28.5 % and 22.5 %, respectively). Finally, β-pinene (31.4 %), germacrene D (23.3 %) and α-pinene (17.5 %) were the most abundant compounds in the needle oil of P. stankewiczii. Additionally the larvicidal and repellent properties of their essential oils were evaluated against Aedes albopictus, a mosquito of great ecological and medical importance. The results of bioassays revealed that repellent abilities of the tested essential oils were more potent than their larvicidal activities. The essential oils of P. brutia, P. halepensis and P. stankewiczii presented considerable larvicidal activity (LC50 values 67.04 mgL(-1) and 70.21 mgL(-1), respectively), while the others were weak to inactive against larvae. The essential oils of P. halepensis, P. brutia, and P. stankewiczii presented a high repellent activity, even at the dose of 0.2 μL cm(-2), while in the dose of 0.4 μL cm(-2), almost all the tested EOs displayed protection against the mosquito.
Department of Pharmacognosy and Chemistry of Natural Products, School of Pharmacy, University of Athens, Panepistimioupoli Zographou, 157 71, Athens, Greece.
Katerina
K
Koutsaviti
Athanassios
A
Giatropoulos
Danae
D
Pitarokili
Dimitrios
D
Papachristos
Antonios
A
Michaelakis
Olga
O
Tzakou
D000330:Aedes / Q000187:drug effects* / Q000254:growth & development; D000818:Animals; D001681:Biological Assay; D002849:Chromatography, Gas; D008401:Gas Chromatography-Mass Spectrometry; D006115:Greece; D007302:Insect Repellents / Q000737:chemistry / Q000494:pharmacology*; D007306:Insecticides / Q000737:chemistry / Q000494:pharmacology*; D007814:Larva / Q000187:drug effects / Q000254:growth & development; D009822:Oils, Volatile / Q000737:chemistry / Q000494:pharmacology*; D028223:Pinus / Q000737:chemistry*; D010938:Plant Oils / Q000737:chemistry / Q000494:pharmacology*
D007302:Insect Repellents; D007306:Insecticides; D009822:Oils, Volatile; D010938:Plant Oils