Effect of fasudil hydrochloride on the post-thaw viability of cryopreserved porcine adipose-derived stem cells.
BACKGROUND
Adipose-derived stem cells (ADSCs) are of interest for regenerative medicine as they are isolated easily and can differentiate into multiple cell lineages. Recently, it was reported that a Rho-associated kinase (ROCK) inhibitor Y-27632 could enhance the post-thaw viability and physiological function of cryopreserved BMSC.


OBJECTIVE
The present study is to investigate whether Fasudil hydrochloride (FH, a selective Rho-kinase inhibitor like Y-27632) can exert a similar beneficial effect on the post-thaw viability of cryopreserved ADSCs.


METHODS
ADSCs were allotted in three cryopreservation solutions [I:10 % (v/v) (DMSO), 30 % (v/v) FBS (as a negative control); II:10µM FH, 10 % (v/v) DMSO, 30 % (v/v) FBS; III:10 µM Y-27632, 10 % (v/v) DMSO, 30 % (v/v) fetal bovine serum, All groups were frozen using a rapid freezing method and stored at -196 degree C in liquid nitrogen for 30 days.After thawing and being cultured 24h,viability of ADSCs were detected by MTT assay.


RESULTS
The MTT assay showed significant differences in the proportion of adherent viable cells over the concentration of Y-27632 and FH, Additionally, FH did not induce morphological changes in the frozen-thawed ADSCs like Y-27632 did.


CONCLUSIONS
FH might represent a promising cryopreservation solution in enhancing the post-thaw viability and physiological function of cryopreserved ADSCs and did not chang the cell appearance.
School of Life Science, FuYang Teachers College, Fuyang, Anhui, China.
Y T
YT
Ji
School of Life Science, FuYang Teachers College, Fuyang, Anhui, China.
D Y
DY
Chen
School of Life Science, FuYang Teachers College, Fuyang, Anhui, China.
S L
SL
Jiang
School of Life Science, FuYang Teachers College, Fuyang, Anhui, China.
C Q
CQ
Qu
D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine / Q000031:analogs & derivatives* / Q000378:metabolism; D000273:Adipose Tissue / Q000166:cytology* / Q000187:drug effects; D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D015925:Cryopreservation / Q000379:methods / Q000662:veterinary*; D003451:Cryoprotective Agents / Q000378:metabolism*; D047428:Protein Kinase Inhibitors / Q000378:metabolism*; D013234:Stem Cells / Q000166:cytology* / Q000187:drug effects; D013552:Swine; D054460:rho-Associated Kinases / Q000037:antagonists & inhibitors
D003451:Cryoprotective Agents; D047428:Protein Kinase Inhibitors; D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; D054460:rho-Associated Kinases; C049347:fasudil