Effect of aminoguanidine on sciatic functional index, oxidative stress, and rate of apoptosis in an experimental rat model of ischemia-reperfusion injury.
This study was conducted to investigate the potential protective effects of aminoguanidine (AG) on sciatic functional index (SFI), oxidative stress status, and apoptosis index using a rat model of experimental sciatic nerve ischemia-reperfusion injury (I/R). Treatment groups received 150 mg AG/kg body mass, 24 h after the induction of ischemia. After reperfusion for 2, 4, 7, 14, and 28 days, we evaluated measured SFI, plasma antioxidant enzymes, total antioxidant capacity (TAC), malondialdehyde (MDA), and index of apoptosis. SFI was significantly improved on the 7th and 14th day of reperfusion in the AG-treated groups. AG treatment resulted in the significant reduction of MDA levels on the 7th and 14th day of reperfusion. TAC was only increased after 7 days of reperfusion compared with the untreated group. SOD activity was decreased in both the untreated and AG-treated groups by comparison with the control, but did not show a significant change. GPx activity decreased only after 7 days of reperfusion. The maximal rate of apoptosis occurred on the 7th day of reperfusion. Treatment with AG significantly reduced this enhancement. AG exhibits positive effects against sciatic nerve I/R injury, possibly in part because of the protective effects of AG against apoptosis and I/R-induced oxidative stress.
Mohsen
M
Alipour
a Department of Physiology & Pharmacology, School of Medicine, Kramandan, Zanjan University of Medical Sciences, 4513956111, Islamic Republic of Iran.
Alipour
A
Mohsen
Farhad
F
Gadiri-Soufi
Gadiri Soufi
GS
Farhad
Mohammad-Reza
MR
Jafari
Jafari
J
Mohammad-Reza
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology* / Q000627:therapeutic use; D017209:Apoptosis / Q000187:drug effects*; D006146:Guanidines / Q000494:pharmacology* / Q000627:therapeutic use; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects*; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000188:drug therapy / Q000473:pathology* / Q000503:physiopathology; D012584:Sciatic Nerve / Q000187:drug effects* / Q000473:pathology / Q000503:physiopathology
D000975:Antioxidants; D006146:Guanidines; C004479:pimagedine
aminoguanidine; indice fonctionnel sciatique; ischemia–reperfusion; ischémie–reperfusion; oxidative stress; rat; sciatic functional index; stress oxydant