A qualitative and quantitative analysis of AgNORs in keratocystic odontogenic tumor, unicystic ameloblastoma and multicystic ameloblastoma.
OBJECTIVE
A comparative evaluation of proliferation activity in unicystic ameloblastoma (UA), multicystic ameloblastoma (MA) and keratocystic odontogenic tumor (KCOT) using silver staining technique.


METHODS
In the present study 21 histopathologically confirmed paraffin blocks,7 each of UA, MA and KCOT were selected and stained with silver nitrate.


METHODS
For quantitative analysis, 100 cells were counted at 1000x magnification for AgNORs and the mean value was calculated. Qualitative analysis of AgNORs included normal (oval shaped) and abnormal groups (bean shaped) in the lesion.


METHODS
The statistical analysis of data was done by a specialist statistician using two way ANOVA and multiple comparisons with Tukey's test in advanced excel.


RESULTS
The AgNOR count was more in KCOT when compared to MA and UA with the pattern of distribution of AgNORs more in basal than in the parabasal layer in KCOT. The qualitative analysis showed small to large oval AgNOR's in KCOT and few clusters in MA whereas in UA irregular clusters were seen.


CONCLUSIONS
This concludes the expediency of AgNOR staining in reflecting the high proliferation rate and a more aggressive behavior of KCOT in comparison to MA and UA which signifies requirement of a more hostile surgical approach in KCOT to avoid recurrences following different treatment modalities.
Professor, Department of Oral and Maxillofacial Pathology, St Joseph Dental College , Duggirala, Eluru, Andhra Pradesh, India .
Anuradha
A
Ananthaneni
Postgraduate Student, Department of Oral and Maxillofacial Pathology, St Joseph Dental College , Duggirala, Eluru, Andhra Pradesh, India .
Urmila
U
Udayashankar
Professor and Head, Department of Oral and Maxillofacial Pathology, St Joseph Dental College , Duggirala, Eluru, Andhra Pradesh, India .
Vijay Srinivasvas
VS
Guduru
Reader, Department of Oral and Maxillofacial Pathology, St Joseph Dental College , Duggirala, Eluru, Andhra Pradesh, India .
V V S
VV
Ramprasad
Reader, Department of Oral and Maxillofacial Pathology, St Joseph Dental College , Duggirala, Eluru, Andhra Pradesh, India .
Sabitha Devi
SD
Ramisetty
Postgraduate Student, Department of Oral and Maxillofacial Pathology, St Joseph Dental College , Duggirala, Eluru, Andhra Pradesh, India .
Srilekha
S
Namala
Postgraduate Student, Department of Oral and Maxillofacial Pathology, St Joseph Dental College , Duggirala, Eluru, Andhra Pradesh, India .
Kiran Kumar Naik
KK
Badavath
AgNORs; KCOT; MA; UA