Multiple-fingerprint analysis for investigating quality control of Flammulina velutipes fruiting body polysaccharides.
Quality control issues overshadow potential health benefits of the edible mushroom Flammulina velutipes, with the detection and isolation of polysaccharides posing particular problems. In this study, multiple-fingerprint analysis was performed using chemometrics to assess polysaccharide quality and antioxidant activity of F. velutipes fruiting bodies from different sources. The authentic source exhibited differences in both oxygen radical absorbance capacity and ferric reducing antioxidant power from foreign sources. IR spectroscopic/HPLC fingerprints of polysaccharide extracts from the authentic source were established and applied to assess the polysaccharide quality of foreign sources. Analysis of IR fingerprints using Pearson correlation coefficient gave correlation coefficient r values of 0.788 and 0.828 for two foreign sources, respectively, indicating distinctness from the authentic source. Analysis of HPLC fingerprints using the supervised method by Traditional Chinese Medicine could not discriminate between sources (r > 0.9), but principal component analysis of IR and HPLC fingerprints distinguished the foreign sources.
Research Center for Food Safety and Nutrition, Key Laboratory of Urban Agriculture (South), Bor S. Luh Food Safety Research Center, School of Agriculture and Biology, Shanghai Jiao Tong University , Shanghai 200240, China.
Pu
P
Jing
Shu-Juan
SJ
Zhao
Man-Man
MM
Lu
Zan
Z
Cai
Jie
J
Pang
Li-Hua
LH
Song
D002851:Chromatography, High Pressure Liquid; D055393:Flammulina / Q000737:chemistry*; D048690:Fruiting Bodies, Fungal / Q000737:chemistry; D011134:Polysaccharides / Q000737:chemistry*; D011786:Quality Control
D011134:Polysaccharides
Flammulina velutipes; antioxidant activity; fingerprint analysis; polysaccharide; principal component analysis; similarity