Suppressor of fused (Sufu) represses Gli1 transcription and nuclear accumulation, inhibits glioma cell proliferation, invasion and vasculogenic mimicry, improving glioma chemo-sensitivity and prognosis.
Glioblastoma are highly aggressive brain tumors with poor prognosis. While various dysregulation of signaling pathways in gliomas have been described, the identification of biomarkers and therapy targets remains an important task for novel diagnostic and therapeutic approaches. Here we described that the Suppressor of fused (also known as Sufu) is significantly down-regulated in high-grade gliomas, correlating with a poor prognosis. We demonstrated that ectopic expression of Sufu inhibited cell proliferation, invasion and vasculogenic mimicry. In addition, overexpression of Sufu reduced Gli reporter gene transcription activity and prevented Gli1 nuclear accumulation, whereas knockdown of Sufu reversed these effects. Furthermore, overexpressed Sufu sensitized glioblastoma to Temozolomide and Cyclopamine. Thus, Sufu is potential tumor suppressor and therapeutic target in glioblastoma.
Department of Neurosurgery, The Second Affiliated Hospital of Harbin Medical University, Harbin, China. Chinese Glioma Cooperation Group (CGCG).
Xing
X
Liu
Department of Neurosurgery, The Second Affiliated Hospital of Harbin Medical University, Harbin, China. Chinese Glioma Cooperation Group (CGCG).
Xiaofeng
X
Wang
Department of Neurosurgery, The Second Affiliated Hospital of Harbin Medical University, Harbin, China. Chinese Glioma Cooperation Group (CGCG).
Wenzhong
W
Du
Department of Neurosurgery, Huashan Hospital, Fudan University, Shanghai, China.
Lingchao
L
Chen
Department of Neurosurgery, The Second Affiliated Hospital of Harbin Medical University, Harbin, China. Chinese Glioma Cooperation Group (CGCG).
Guangzhi
G
Wang
Department of Neurosurgery, The Second Affiliated Hospital of Harbin Medical University, Harbin, China. Chinese Glioma Cooperation Group (CGCG).
Yuqiong
Y
Cui
Department of Neurosurgery, The Second Affiliated Hospital of Harbin Medical University, Harbin, China. Chinese Glioma Cooperation Group (CGCG).
Yang
Y
Liu
Department of Neurosurgery, The Second Affiliated Hospital of Harbin Medical University, Harbin, China. Chinese Glioma Cooperation Group (CGCG).
Zhijin
Z
Dou
Department of Neurosurgery, The Second Affiliated Hospital of Harbin Medical University, Harbin, China. Chinese Glioma Cooperation Group (CGCG).
Hongjun
H
Wang
Department of Neurosurgery, The Second Affiliated Hospital of Harbin Medical University, Harbin, China. Chinese Glioma Cooperation Group (CGCG).
Ping
P
Zhang
Department of Neurosurgery, The Second Affiliated Hospital of Harbin Medical University, Harbin, China. Chinese Glioma Cooperation Group (CGCG).
Liang
L
Chang
Department of Neurosurgery, The Second Affiliated Hospital of Harbin Medical University, Harbin, China. Chinese Glioma Cooperation Group (CGCG).
Liye
L
Yi
Department of Neurosurgery, The Second Affiliated Hospital of Harbin Medical University, Harbin, China. Chinese Glioma Cooperation Group (CGCG).
Jinquan
J
Cai
Department of Neurosurgery, The Second Affiliated Hospital of Harbin Medical University, Harbin, China. Chinese Glioma Cooperation Group (CGCG).
Chuanlu
C
Jiang
D000328:Adult; D000818:Animals; D001932:Brain Neoplasms / Q000378:metabolism*; D045744:Cell Line, Tumor; D002467:Cell Nucleus / Q000378:metabolism*; D049109:Cell Proliferation; D003606:Dacarbazine / Q000008:administration & dosage / Q000031:analogs & derivatives; D018450:Disease Progression; D015536:Down-Regulation; D005260:Female; D005910:Glioma / Q000188:drug therapy / Q000378:metabolism*; D006801:Humans; D008297:Male; D051379:Mice; D008819:Mice, Nude; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D009368:Neoplasm Transplantation; D011379:Prognosis; D034741:RNA, Small Interfering / Q000378:metabolism; D012097:Repressor Proteins / Q000378:metabolism*; D000077204:Temozolomide; D014157:Transcription Factors / Q000378:metabolism*; D016896:Treatment Outcome; D014704:Veratrum Alkaloids / Q000008:administration & dosage; D000071676:Zinc Finger Protein GLI1
C400551:GLI1 protein, human; D034741:RNA, Small Interfering; D012097:Repressor Proteins; C490764:SUFU protein, human; D014157:Transcription Factors; D014704:Veratrum Alkaloids; D000071676:Zinc Finger Protein GLI1; D003606:Dacarbazine; D000077204:Temozolomide; C000541:cyclopamine