The effect of weight reduction on antioxidant enzymes and their association with dietary intake of vitamins A, C and E.
OBJECTIVE
Our goal was to assess the effects of weight loss on antioxidant enzymes of red blood cells and it's relation with vitamins A, E and C intake in 30 obese women.


METHODS
General information, anthropometric measurements, 3-day food recall, and fasting blood samples were collected from 30 obese women at the beginning of the study and after 3 months intervention. Weight loss was set at about 10% of their weight before the intervention.


RESULTS
Glutathione reductase and catalase activities showed a significant increase (P < 0.01) after weight reduction, but no significant changes were seen in the superoxide dismutase and glutathione peroxidase activities. There was a positive linear correlation between daily vitamin C intake with superoxide dismutase enzyme after intervention (P = 0.004, r = 0.507). There was a negative linear correlation between vitamin E intake and glutathione peroxidase activity before intervention (P = 0.005, r = -0.5). A negative correlation was found between daily vitamin A intake and glutathione reductase enzyme before and after intervention (r = -0.385, r = -0.397, P < 0.05) respectively. No significant correlation was observed between vitamins A, C, E amounts and catalase activity.


CONCLUSIONS
Ten percent weight reduction can have a significant role in increasing antioxidant enzymes activities, especially glutathione reductase, and catalase enzymes in obese women. However, it is important to take into consideration a balanced amount of certain nutrients while administering a diet with limited energy.
Department of Cellular and Molecular Nutrition, School of Nutritional Sciences and Dietetic, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
Masoud
M
Ramezanipour
Department of Cellular and Molecular Nutrition, School of Nutritional Sciences and Dietetic, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
Mahmood
M
Jalali
Department of Community Nutrition, School of Nutritional Sciences and Dietetic, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
Haleh
H
Sadrzade-Yeganeh
Department of Clinical Nutrition, School of Nutritional Sciences and Dietetic, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
Seyed Ali
SA
Keshavarz
Department of Epidemiology and Biostatistics, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
Mohammad Reza
MR
Eshraghian
Department of Community Nutrition, School of Nutritional Sciences and Dietetic, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
Minoo
M
Bagheri
Tehran University of Medical Sciences, Tehran, Iran.
Sara Seyed
SS
Emami
D000328:Adult; D000975:Antioxidants / Q000378:metabolism; D001205:Ascorbic Acid / Q000008:administration & dosage* / Q000378:metabolism; D001835:Body Weight / Q000502:physiology; D031204:Caloric Restriction; D002374:Catalase / Q000097:blood; D005260:Female; D005979:Glutathione Peroxidase / Q000097:blood; D005980:Glutathione Reductase / Q000097:blood; D006454:Hemoglobins / Q000032:analysis; D006801:Humans; D008875:Middle Aged; D065228:Non-Randomized Controlled Trials as Topic / Q000379:methods; D009765:Obesity / Q000178:diet therapy*; D010088:Oxidoreductases / Q000032:analysis / Q000378:metabolism*; D013482:Superoxide Dismutase / Q000097:blood; D014801:Vitamin A / Q000008:administration & dosage* / Q000378:metabolism; D014810:Vitamin E / Q000008:administration & dosage* / Q000378:metabolism*; D015431:Weight Loss / Q000187:drug effects / Q000502:physiology*; D055815:Young Adult
D000975:Antioxidants; D006454:Hemoglobins; D014801:Vitamin A; D014810:Vitamin E; D010088:Oxidoreductases; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005980:Glutathione Reductase; D001205:Ascorbic Acid