Effect of induced peritoneal endometriosis on oocyte and embryo quality in a mouse model.
OBJECTIVE
To assess the impact of peritoneal endometriosis on oocyte and embryo quality in a mouse model.


METHODS
Peritoneal endometriosis was surgically induced in 33 B6CBA/F1 female mice (endometriosis group, N = 17) and sham-operated were used as control (sham group, N = 16). Mice were superovulated 4 weeks after surgery and mated or not, to collect E0.5-embryos or MII-oocytes. Evaluation of oocyte and zygote quality was done by immunofluorescence under spinning disk confocal microscopy.


RESULTS
Endometriosis-like lesions were observed in all mice of endometriosis group. In both groups, a similar mean number of MII oocytes per mouse was observed in non-mated mice (30.2 vs 32.6), with a lower proportion of normal oocytes in the endometriosis group (61 vs 83 %, p < 0.0001). Abnormalities were incomplete extrusion or division of the first polar body and spindle abnormalities. The mean number of zygotes per mouse was lower in the endometriosis group (21 vs 35.5, p = 0.02) without difference in embryo quality.


CONCLUSIONS
Our results support that induced peritoneal endometriosis in a mouse model is associated with a decrease in oocyte quality and embryo number. This experimental model allows further studies to understand mechanisms of endometriosis-associated infertility.
Service de Gynécologie-Obstétrique, Hôpital Tenon, Assistance Publique des Hôpitaux de Paris, Université Pierre et Marie Curie Paris 6, GRC 6-UPMC Centre Expert en Endométriose (C3E), Paris, France, drcohenjonathan@gmail.com.
J
J
Cohen
A
A
Ziyyat
I
I
Naoura
N
N
Chabbert-Buffet
S
S
Aractingi
E
E
Darai
B
B
Lefevre
D000818:Animals; D004195:Disease Models, Animal; D004622:Embryo, Mammalian / Q000473:pathology; D004715:Endometriosis / Q000209:etiology / Q000473:pathology* / Q000601:surgery; D005260:Female; D008815:Mice, Inbred Strains; D009865:Oocytes / Q000473:pathology*; D010532:Peritoneal Diseases / Q000209:etiology / Q000473:pathology*; D015053:Zygote / Q000502:physiology