Hypoglycaemic effect of galactooligosaccharides in alloxan-induced diabetic rats.
This study was conducted to assess the effect of prebiotic galactooligosaccharides (GOS) on alloxan-induced diabetes in male Sprague-Dawley (SD) rats. Diabetes was induced by administration of alloxan (100 mg/kg) and rats were divided in 4 groups: normal control group (NCG), prebiotic control group (PCG), diabetic control group (DCG) and diabetic prebiotic group (DPG). While PCG and DPG were fed with GOS supplemented (10% w/w) diet, NCG and DCG were administered with basal diet. Rats were sacrificed after 42 d for collection of blood and liver. Faecal samples were collected at the interval of 7 d throughout the study for measurement of lactobacilli and coliform count. Feeding of GOS decreased or delayed the severity of diabetes by amelioration of diabetes associated markers including fasting blood glucose, haemoglobin, glycosylated haemoglobin triglycerides, total cholesterol, low density lipoproteins, creatinine and urea. GOS was also found to improve the levels of antioxidative enzymes (superoxide dismutase, catalase and glutathione peroxidase) in liver and blood. Improvement in lactobacilli count along with a concomitant decrease in coliform count was observed in GOS fed groups.
Dairy Microbiology Division,National Dairy Research Institute,Karnal 132001,India.
Vikas
V
Sangwan
Dairy Microbiology Division,National Dairy Research Institute,Karnal 132001,India.
Sudhir K
SK
Tomar
Dairy Microbiology Division,National Dairy Research Institute,Karnal 132001,India.
Babar
B
Ali
Dairy Technology Division,National Dairy Research Institute,Karnal 132001,India.
Ram R B
RR
Singh
Dairy Technology Division,National Dairy Research Institute,Karnal 132001,India.
Ashish K
AK
Singh
D000818:Animals; D016667:Bacterial Capsules; D001786:Blood Glucose / Q000032:analysis; D002374:Catalase / Q000032:analysis / Q000097:blood; D003921:Diabetes Mellitus, Experimental / Q000097:blood* / Q000188:drug therapy*; D005215:Fasting; D005243:Feces / Q000382:microbiology; D005690:Galactose / Q000008:administration & dosage*; D005979:Glutathione Peroxidase / Q000032:analysis / Q000097:blood; D006442:Glycated Hemoglobin / Q000032:analysis; D006454:Hemoglobins / Q000032:analysis; D007004:Hypoglycemic Agents / Q000008:administration & dosage*; D007778:Lactobacillus / Q000302:isolation & purification; D008055:Lipids / Q000097:blood; D008099:Liver / Q000201:enzymology; D008297:Male; D009844:Oligosaccharides / Q000008:administration & dosage*; D056692:Prebiotics; D051381:Rats; D017207:Rats, Sprague-Dawley; D013482:Superoxide Dismutase / Q000032:analysis / Q000097:blood
D001786:Blood Glucose; D002374:Catalase; D005690:Galactose; D005979:Glutathione Peroxidase; D006442:Glycated Hemoglobin; D006454:Hemoglobins; D007004:Hypoglycemic Agents; D008055:Lipids; D009844:Oligosaccharides; D056692:Prebiotics; D013482:Superoxide Dismutase
Galactooligosaccharides; diabetes; hyperglycaemia; oxidative stress; prebiotics