Improved detection of Mycobacterium bovis in bovine tissues using immunomagnetic separation approaches.
Immunomagnetic separation (IMS) represents a simple but effective method of selectively capturing and concentrating Mycobacterium bovis, the causative agent of bovine tuberculosis (bTB), from tissue samples. It is a physical cell separation technique that does not impact cell viability, unlike traditional chemical decontamination prior to culture. IMS is performed with paramagnetic beads coated with M. bovis-specific antibody and peptide binders. Once captured by IMS, M. bovis cells can be detected by either PCR or cultural detection methods. Increased detection rates of M. bovis, particularly from non-visibly lesioned lymph node tissues from bTB reactor animals, have recently been reported when IMS-based methods were employed.
Institute for Global Food Security, School of Biological Sciences, Queen's University Belfast,, Medical Biology Centre, 97 Lisburn Road, Belfast BT9 7BL, Northern Ireland, UK, i.grant@qub.ac.uk.
Irene R
IR
Grant
Linda D
LD
Stewart
D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis* / Q000382:microbiology*; D018189:Immunomagnetic Separation / Q000379:methods*; D025202:Molecular Diagnostic Techniques / Q000379:methods* / Q000592:standards*; D009163:Mycobacterium bovis / Q000235:genetics* / Q000302:isolation & purification*; D014376:Tuberculosis / Q000662:veterinary*