Histological and Histometrical Evaluation of two Synthetic Hydroxyapatite Based Biomaterials in the Experimental Periodontal Defects in Dogs.
OBJECTIVE
The present study was to evaluate histologically and histometrically the efficacy of Chitra granules in the regeneration of alveolar bone and to compare it with that of OsteoGenR (HA Resorb)(TM) in iatrogenically created alveolar bone defects in mongrel dogs.


METHODS
Four dogs (16 sites) were used for this split-mouth study. The animals were divided randomly into two groups of two animals. Same animals were used as control and test. Each dog had four implantation sites. The periodontal defects were prepared by acute defect model. Animals were sacrificed at 3 months (n=2), 6 months (n=2) and histologic and histometric evaluation was carried out.


METHODS
The data was analysed using statistical package Graph pad Software. Comparison of the hard and soft tissue parameters in the two groups was done using the Wilcoxan (Man Whitney), two tailed t-test. A p-value less than 0.05 were considered significant.


RESULTS
Maturing bone with immature periodontal ligament fibers were observed at three months and advanced osteogenesis at six months with both the types of bone graft materials. The mean values showed that amount of new bone formed with OsteoGenR (HA Resorb)(TM) was slightly more than that obtained by Chitra granules in histometric evaluation.


CONCLUSIONS
Histological study showed similar healing pattern with both the types of bone graft materials with maturing bone at 3 months and advanced osteogenesis at six months in experimental intraosseous periodontal defects in dogs. However, histological evaluation for longer period is necessary to determine the time taken for complete replacement of the bone graft materials with new bone.
Professor, Department of Periodontics, Annoor Dental College , Ernakulam, India .
Johnson Prakash
JP
D'lima
Professor, Department of Periodontics, Annoor Dental College , Ernakulam, India .
Jose
J
Paul
Senior Lecturer, Department of Periodontics, Annoor Dental College , Ernakulam, India .
Plato
P
Palathingal
Professor, Department of Periodontics, Manipal College of Dental Surgery , Manipal, India .
Brr
B
Varma
Professor, Department of Periodontics, Manipal College of Dental Surgery , Manipal, India .
Mahalinga
M
Bhat
Scientist, Department of Pathophysiology, Sri Chitra Tirunal Institiute of Medical Science and Technology , Trivandrum, India .
Mira
M
Mohanty
Chitra granules; Intrabony alveolar bone defects; Mongrel dogs; Osteogenesis; Osteogenr (Ha Resorb)TM