Isolation of a novel beta-cypermethrin degrading strain Bacillus subtilis BSF01 and its biodegradation pathway.
Continuous use of the pyrethroid insecticide beta-cypermethrin (beta-cp) has resulted in serious environmental contamination problems. We report here that a novel bacterial strain BSF01, which was isolated from activated sludge and identified as Bacillus subtilis (collection number: CCTCC AB 2014103), showed high efficiency in degrading beta-cp. Strain BSF01 was able to utilize beta-cp as the sole carbon source for growth and degraded 89.4 % of 50 mg L(-1) beta-cp within 7 days. The optimal conditions for beta-cp degradation were determined to be 34.5 °C, pH 6.7, and inocula amount 0.11 g dry wt L(-1) using response surface methodology. The kinetic parameters q max, K s, and K i were established to be 2.19 day(-1), 76.37 mg L(-1), and 54.14 mg L(-1), respectively. The critical inhibitor concentration was determined to be 64.30 mg L(-1). Seven metabolites were identified by gas chromatography-mass spectrometry. Furthermore, a novel biodegradation pathway for beta-cp was proposed on the basis of analysis of the metabolites. This strain was also capable of degrading a wide range of pyrethroid insecticides including cypermethrin, deltamethrin, cyhalothrin, and beta-cyfluthrin, which similar to beta-cp are hazardous chemicals. Taken together, our results depict the biodegradation pathway of beta-cp and highlight the promising potentials of strain BSF01 in bioremediation of pyrethroid-contaminated environments.
Laboratory of Insect Toxicology, and Key Laboratory of Pesticide and Chemical Biology, Ministry of Education, South China Agricultural University, Guangzhou, 510642, People's Republic of China.
Ying
Y
Xiao
Shaohua
S
Chen
Yuanqi
Y
Gao
Wei
W
Hu
Meiying
M
Hu
Guohua
G
Zhong
D001412:Bacillus subtilis / Q000302:isolation & purification* / Q000378:metabolism*; D001673:Biodegradation, Environmental; D008401:Gas Chromatography-Mass Spectrometry; D007306:Insecticides / Q000737:chemistry*; D009570:Nitriles / Q000737:chemistry; D011722:Pyrethrins / Q000737:chemistry*; D012722:Sewage / Q000382:microbiology
D007306:Insecticides; D009570:Nitriles; D011722:Pyrethrins; D012722:Sewage; C017160:cypermethrin; C017180:decamethrin; C052570:cyfluthrin; C037304:cyhalothrin