Different structures of heterogeneous starch granules from high-amylose rice.
High-amylose cereal starches usually have heterogeneous starch granules in morphological structure. In the present study, the polygonal, aggregate, elongated, and hollow starch granules were separated from different regions of the kernels of high-amylose rice, and their structures were investigated. The results showed that the polygonal starch granules had low amylose content and high short branch-chain and branching degree of amylopectin, and exhibited A-type crystallinity. The aggregate starch granules had high long branch-chain of amylopectin, relative crystallinity, and double helix content, and exhibited C-type crystallinity. The elongated starch granules had high amylose content and low branching degree of amylopectin and relative crystallinity, and exhibited C-type crystallinity. The hollow starch granules had very high amylose content, proportion of amorphous conformation, and amylose-lipid complex, and very low branch-chain of amylopectin, branching degree of amylopectin, and double helix content, and exhibited no crystallinity. The different structures of heterogeneous starch granules from high-amylose rice resulted in significantly different thermal properties.
Key Laboratories of Crop Genetics and Physiology of the Jiangsu Province and Plant Functional Genomics of the Ministry of Education, ‡Co-Innovation Center for Modern Production Technology of Grain Crops, and §Testing Center, Yangzhou University , Yangzhou 225009, China.
Jianmin
J
Man
Lingshang
L
Lin
Zhifeng
Z
Wang
Youping
Y
Wang
Qiaoquan
Q
Liu
Cunxu
C
Wei
D000688:Amylose / Q000737:chemistry*; D003460:Crystallization; D012275:Oryza / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry*; D013213:Starch / Q000737:chemistry; D014961:X-Ray Diffraction
D010936:Plant Extracts; D013213:Starch; D000688:Amylose
crystalline structure; heterogeneous starch granule; high-amylose rice; lamellar structure; molecular weight distribution; thermal property