In vitro selection of a peptide aptamer that changes fluorescence in response to verotoxin.
A peptide aptamer that changes fluorescence upon binding to verotoxin was selected in vitro using ribosome display with a tRNA carrying an environment-sensitive fluorescent probe. The aptamer specifically bound to verotoxin with a dissociation constant (K d) of 3.94 ± 1.6 µM, and the fluorescence decreased by 78% as the verotoxin concentration was increased. The selected peptide can be used for detection of verotoxin.
Nano Medical Engineering Laboratory, RIKEN, 2-1 Hirosawa, Wako, Saitama, 351-0198, Japan, yasodha@riken.jp.
Yasodha
Y
Manandhar
K C Tara
KC
Bahadur
Wei
W
Wang
Takanori
T
Uzawa
Toshiro
T
Aigaki
Yoshihiro
Y
Ito
D052158:Aptamers, Peptide / Q000302:isolation & purification* / Q000378:metabolism*; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D005453:Fluorescence; D005470:Fluorometry / Q000379:methods*; D011485:Protein Binding; D022603:Shiga Toxins / Q000032:analysis*
D052158:Aptamers, Peptide; D022603:Shiga Toxins