Structural and compositional changes in the salivary pellicle induced upon exposure to SDS and STP.
Sodium dodecyl sulphate (SDS) and sodium tripolyphosphate (STP) act to remove stained pellicle from dentition and loosen deposits on tooth surfaces that may become cariogenic over time. This study investigated how SDS and STP impact the salivary pellicle adsorbed onto hydroxyapatite and silica sensors using a dual polarisation interferometer and a quartz-crystal microbalance with dissipation. After the pellicle was exposed to SDS and STP the remaining pellicle, although weaker, due to the loss of material, became less dense but with a higher elastic component; suggesting that the viscous component of the pellicle was being removed. This would imply a structural transformation from a soft but dense structured pellicle, to a more diffuse pellicle. In addition, the majority of proteins displaced by both SDS and STP were identified as being acidic in nature; implying that the negatively charged groups of SDS and STP may be responsible for the displacement of the pellicle proteins observed.
a Institute of Food Research , Colney , Norwich , UK.
Anthony
A
Ash
Francis
F
Mulholland
Gary R
GR
Burnett
Peter J
PJ
Wilde
D000328:Adult; D002853:Chromatography, Liquid; D044622:Dental Pellicle / Q000737:chemistry*; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D011122:Polyphosphates / Q000737:chemistry*; D058525:Quartz Crystal Microbalance Techniques; D012463:Saliva / Q000737:chemistry; D012471:Salivary Proteins and Peptides / Q000737:chemistry*; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry*; D053719:Tandem Mass Spectrometry; D055815:Young Adult
D011122:Polyphosphates; D012471:Salivary Proteins and Peptides; D012967:Sodium Dodecyl Sulfate; C005692:triphosphoric acid
DPI; QCM-D; SDS; mass spectrometry; salivary pellicle; sodium tripolyphosphate