The pepsin digestibility of thermal gel products made from white croaker (Pennahia argentata) muscle in associating with myosin polymerization levels.
Thermal gels were made from white croaker (Pennahia argentata) surimi at various polymerization levels of myosin heavy chains induced by suwari treatment at 38 °C for various time periods and subsequently heated at 85 °C for 20 min. Myosin heavy chain polymerization levels were also achieved in the presence of microbial transglutaminase (MTG) added at various concentrations in the surimi. The breaking strength and breaking strain rate were markedly increased during suwari treatment up to 60 min in accordance with the increased levels of myosin heavy chain polymerization. MTG enhanced myosin heavy chain polymerization during suwari treatment for 15 and 30 min, resulting in the increase of breaking strength. The solubilization in 8 M urea and pepsin digestibility of these gels as well as angiotensin I-converting enzyme (ACE) inhibitory activity of their pepsin digests were decreased with the increased levels of myosin heavy chain polymerization. These results suggest that myosin heavy chain polymerization affects not only rheological properties of thermal gels but also their functional properties for human health.
Fish Protein Laboratory, Research and Development Center, Suzuhiro Kamaboko Honten Co., Ltd, Odawara, Kanagawa, 250-0862, Japan.
Nobuhiko
N
Ueki
Jianrong
J
Wan
Shugo
S
Watabe
D000806:Angiotensin-Converting Enzyme Inhibitors / Q000378:metabolism; D000818:Animals; D004063:Digestion; D004591:Electrophoresis, Polyacrylamide Gel; D005782:Gels / Q000737:chemistry*; D006358:Hot Temperature; D009132:Muscles / Q000737:chemistry*; D018995:Myosin Heavy Chains / Q000737:chemistry*; D010434:Pepsin A / Q000378:metabolism*; D007703:Peptidyl-Dipeptidase A / Q000378:metabolism; D010473:Perciformes; D058105:Polymerization; D011503:Transglutaminases / Q000378:metabolism
D000806:Angiotensin-Converting Enzyme Inhibitors; D005782:Gels; D011503:Transglutaminases; D007703:Peptidyl-Dipeptidase A; D010434:Pepsin A; D018995:Myosin Heavy Chains
myosin heavy chain polymerization; pepsin digestibility; thermal gel; transglutaminase; white croaker