In situ hybridization with labeled probes: assessment of african Swine Fever virus in formalin-fixed paraffin-embedded tissues.
In situ hybridization (ISH) has become a very valuable molecular diagnostic tool to detect specific DNA or RNA sequences in biological samples through the use of complementary DNA- or RNA-labeled probes. Here, we describe an optimized in situ hybridization protocol to detect African swine fever virus (ASFV) DNA in formalin-fixed, paraffin-embedded tissues using digoxigenin-labeled probes.
Centre de Recerca en Sanitat Animal (CReSA), UAB-IRTA, Campus de la Universitat Autònoma de Barcelona, Cerdanyola del Vallès, 08193, Bellaterra, Spain, mariabal77@hotmail.com.
Maria
M
Ballester
Fernando
F
Rodríguez
D000357:African Swine Fever / Q000175:diagnosis* / Q000821:virology*; D000358:African Swine Fever Virus / Q000235:genetics*; D000818:Animals; D017403:In Situ Hybridization / Q000295:instrumentation / Q000379:methods*; D015341:Nucleic Acid Probes; D013552:Swine
D015341:Nucleic Acid Probes