Identification and evaluation of strain B37 of Bacillus subtilis antagonistic to sapstain fungi on poplar wood.
Devaluation of poplar products by sapstain accounts for huge and unpredictable losses each year in China. We had isolated four poplar sapstain fungi, Ceratocystis adiposa Hz91, Lasiodiplodia theobromae YM0737, L. theobromae Fx46, and Fusarium sp. YM05, from five poplar varieties and 13 antagonistic bacteria from nine diverse varieties. After being experimented with agar plates, wood chips, and enzyme activities, strain B37 was identified as the best poplar sapstain biocontrol bacterium. The strain B37 was identified as Bacillus subtilis using sequences of the 16S rRNA gene, physiological biochemical, and morphological characteristics.
Key Laboratory of Agricultural Environmental Microbiology, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China ; Jiangsu Polytechnic College of Agriculture and Forestry, Jurong, Jiangsu 212400, China.
XiaoHua
X
Zhang
Jiangsu Polytechnic College of Agriculture and Forestry, Jurong, Jiangsu 212400, China.
GuiHua
G
Zhao
The Connecticut Agricultural Experiment Station Valley Laboratory, 153 Cook Hill Road, Windsor, CT 06095, USA.
DeWei
D
Li
Key Laboratory of Agricultural Environmental Microbiology, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China.
ShunPeng
S
Li
Key Laboratory of Agricultural Environmental Microbiology, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China.
Qing
Q
Hong
D000935:Antifungal Agents / Q000302:isolation & purification* / Q000494:pharmacology; D001412:Bacillus subtilis / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification*; D003904:Mitosporic Fungi / Q000187:drug effects / Q000254:growth & development / Q000302:isolation & purification*; D032107:Populus; D013045:Species Specificity; D014934:Wood / Q000382:microbiology*
D000935:Antifungal Agents