Comparative analysis of colorimetric staining in skin using open-source software.
Colorimetric staining techniques such as immunohistochemistry (IHC), immunofluorescence (IF) and histochemistry (HC) provide useful information regarding the localization and relative amount of a molecule/substance in skin. We have developed a novel, straightforward method to assess colorimetric staining by combining features from two open-source software programs. As a proof of principle, we demonstrate the utility of this approach by analysing changes in skin melanin deposition during the radiation-induced tanning response of Yucatan mini-pigs. This method includes a visualization step to validate the accuracy of colour selection before quantitation to ensure accuracy. The data show that this method is robust and will provide a means to obtain accurate comparative analyses of staining in IHC/IF/HC samples.
Department of Radiation Oncology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.
Paul C
PC
Billings
Jenine K
JK
Sanzari
Ann R
AR
Kennedy
Keith A
KA
Cengel
John T
JT
Seykora
D000818:Animals; D001706:Biopsy; D003124:Colorimetry / Q000379:methods*; D004817:Epidermis / Q000378:metabolism; D007091:Image Processing, Computer-Assisted; D007150:Immunohistochemistry; D008543:Melanins / Q000737:chemistry*; D008856:Microscopy, Fluorescence; D012867:Skin / Q000378:metabolism*; D012984:Software; D013552:Swine; D013556:Swine, Miniature
D008543:Melanins
analysis; histochemistry; immunofluorescence; immunohistochemistry; method