Dual modality optical coherence and whole-body photoacoustic tomography imaging of chick embryos in multiple development stages.
Chick embryos are an important animal model for biomedical studies. The visualization of chick embryos, however, is limited mostly to postmortem sectional imaging methods. In this work, we present a dual modality optical imaging system that combines swept-source optical coherence tomography and whole-body photoacoustic tomography, and apply it to image chick embryos at three different development stages. The explanted chick embryos were imaged in toto with complementary contrast from both optical scattering and optical absorption. The results serve as a prelude to the use of the dual modality system in longitudinal whole-body monitoring of chick embryos in ovo.
Center for Medical Physics and Biomedical Engineering, Medical University of Vienna, AKH 4L, Währinger Gürtel 18-20, Vienna 1090, Austria.
Mengyang
M
Liu
Center for Anatomy and Cell Biology, Medical University of Vienna, Währinger Straße 13, Vienna 1090, Austria.
Barbara
B
Maurer
Center for Medical Physics and Biomedical Engineering, Medical University of Vienna, AKH 4L, Währinger Gürtel 18-20, Vienna 1090, Austria.
Boris
B
Hermann
Center for Medical Physics and Biomedical Engineering, Medical University of Vienna, AKH 4L, Währinger Gürtel 18-20, Vienna 1090, Austria.
Behrooz
B
Zabihian
Department of Ophthalmology, University of Pittsburgh, Eye & Ear Institute, 203 Lothrop Street, Pittsburgh, PA 15213, USA.
Michelle G
MG
Sandrian
Center for Medical Physics and Biomedical Engineering, Medical University of Vienna, AKH 4L, Währinger Gürtel 18-20, Vienna 1090, Austria.
Angelika
A
Unterhuber
Center for Medical Physics and Biomedical Engineering, Medical University of Vienna, AKH 4L, Währinger Gürtel 18-20, Vienna 1090, Austria.
Bernhard
B
Baumann
Department of Medical Physics and Bioengineering, University College London, Shropshire House, 11-20 Capper Street, London WC1E 6JA, UK.
Edward Z
EZ
Zhang
Department of Medical Physics and Bioengineering, University College London, Shropshire House, 11-20 Capper Street, London WC1E 6JA, UK.
Paul C
PC
Beard
Center for Anatomy and Cell Biology, Medical University of Vienna, Währinger Straße 13, Vienna 1090, Austria.
Wolfgang J
WJ
Weninger
Center for Medical Physics and Biomedical Engineering, Medical University of Vienna, AKH 4L, Währinger Gürtel 18-20, Vienna 1090, Austria.
Wolfgang
W
Drexler
(170.3880) Medical and biological imaging; (170.4500) Optical coherence tomography; (170.5120) Photoacoustic imaging