Evaluation of the effects of antibiotics on cytotoxicity of EGFP and DsRed2 fluorescent proteins used for stable cell labeling.
The use of fluorescent markers has proven to be an attractive tool in biological imaging. However, its usefulness may be confined by the cytotoxicity of the fluorescent proteins. In this article, for the first time, we have examined an influence of the antibiotics present in culture medium on cytotoxicity of the EGFP and DsRed2 markers used for whole-cell labeling. Results showed that doxycycline negatively affected albumin synthesis in DsRed2-expressing hepatoma cells, and that both hepatoma cells and human skin fibroblasts, labeled with this protein, were characterized by the lowered growth rates. Thus, the cytotoxic effect of fluorescent markers depends on both protein used for cell labeling and on growth conditions that may cause cell stress.
Department of Hybrid Microbiosystems Engineering, Nałęcz Institute of Biocybernetics and Biomedical Engineering, PAS, Warsaw, Poland.
Karolina Ewa
KE
Zakrzewska
Department of Hybrid Microbiosystems Engineering, Nałęcz Institute of Biocybernetics and Biomedical Engineering, PAS, Warsaw, Poland.
Anna
A
Samluk
Department of Hybrid Microbiosystems Engineering, Nałęcz Institute of Biocybernetics and Biomedical Engineering, PAS, Warsaw, Poland.
Krzysztof Dariusz
KD
Pluta
Department of Hybrid Microbiosystems Engineering, Nałęcz Institute of Biocybernetics and Biomedical Engineering, PAS, Warsaw, Poland.
Dorota Genowefa
DG
Pijanowska
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002460:Cell Line; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D049452:Green Fluorescent Proteins / Q000009:adverse effects*; D006801:Humans; D008164:Luminescent Proteins / Q000009:adverse effects*
D000900:Anti-Bacterial Agents; D008164:Luminescent Proteins; D049452:Green Fluorescent Proteins