The Effect of Vitamin D Supplementation on Thrombin Generation Assessed by the Calibrated Automated Thrombogram.
Observational and in vitro studies suggest that vitamin D may have antithrombotic activity. This study aimed to examine the relationship between vitamin D supplementation and thrombin generation. Serum 25-hydroxyvitamin D (25(OH)D) and thrombin generation parameters were measured in 73 healthy volunteers. Participants with serum 25(OH)D <50 nmol/L (n = 53) were treated with vitamin D3and tested for 25(OH)D and thrombin generation at the end of treatment. Lag time and time to peak decreased after treatment by a mean of -0.49 ± 0.51 minute (P< .001) and -0.76 ± 0.70 minute (P< .001), respectively, whereas endogenous thrombin potential and peak height increased after treatment by a mean of 170.1 ± 339.8 nmol/L minute (P= .001) and 34.2 ± 47.8 nmol/L (P< .001), respectively. Treatment with vitamin D supplementation seems to have prothrombotic effect in patients with vitamin D insufficiency. These findings should be interpreted with caution and need to be replicated in future studies.
Department of Internal Medicine C, Ha'emek Medical Center, Afula, Israel Bruce Rappaport Faculty of Medicine, Technion-Israel Institute of Technology, Haifa, Israel saliba_wa@clalit.org.il.
Walid
W
Saliba
Department of Internal Medicine A, Ha'emek Medical Center, Afula, Israel.
Karem
K
Awad
Department of Internal Medicine C, Ha'emek Medical Center, Afula, Israel.
Gilat
G
Ron
Department of Internal Medicine C, Ha'emek Medical Center, Afula, Israel Bruce Rappaport Faculty of Medicine, Technion-Israel Institute of Technology, Haifa, Israel.
Mazen
M
Elias
D000328:Adult; D000818:Animals; D057205:Automation, Laboratory; D045744:Cell Line, Tumor; D003430:Cross-Sectional Studies; D019587:Dietary Supplements; D005260:Female; D006801:Humans; D008297:Male; D051381:Rats; D013917:Thrombin / Q000378:metabolism*; D013918:Thrombin Time; D054556:Venous Thromboembolism / Q000097:blood*; D014807:Vitamin D / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000493:pharmacokinetics
D014807:Vitamin D; C104450:25-hydroxyvitamin D; D013917:Thrombin
25(OH)D; calibrated automated thrombogram; coagulation; thrombin generation; thrombosis; vitamin D