Stability and binding effects of silver(I) complexes at lipoxygenase-1.
An anti-inflammatory complex of Ag(I), namely [Ag(tpp)3(asp)](dmf) [tpp = triphenylphosphine, aspH = aspirin, dmf = N,N-dimethylformamide], was synthesized in an attempt to develop novel metallotherapeutic molecules. STD (1)H NMR experiments were used to examine if this complex binds to LOX-1. The (1)H NMR spectra in buffer Tris/D2O betrayed the existence of two complexes: the complex of aspirin and the complex of salicylic acid produced after deacetylation of aspirin. Nevertheless, the STD spectra showed that only the complex of salicylic acid is bound to the enzyme. Molecular docking and dynamics were used to complement our study. The complexes were stabilized inside a large LOX-1 cavity by establishing a network of hydrogen bonds and steric interactions. The complex formation with salicylic acid was more favorable. The in silico results provide a plausible explanation of the experimental results, which showed that only the complex with salicylic acid enters the binding cavity.
Organic Chemistry Laboratory, Department of Chemistry, University of Athens , Panepistimiopolis-Zografou, Athens , Greece .
Eleni
E
Vrontaki
Georgios
G
Leonis
Aggelos
A
Avramopoulos
Manthos G
MG
Papadopoulos
Mihael
M
Simčič
Simona Golič
SG
Grdadolnik
Antreas
A
Afantitis
Georgia
G
Melagraki
Sotiris K
SK
Hadjikakou
Thomas
T
Mavromoustakos
D008084:Lipoxygenase / Q000737:chemistry / Q000378:metabolism*; D062105:Molecular Docking Simulation; D056004:Molecular Dynamics Simulation; D066244:Proton Magnetic Resonance Spectroscopy; D012834:Silver / Q000737:chemistry / Q000378:metabolism*
D012834:Silver; D008084:Lipoxygenase
Anti-inflammatory activity; LOX-1; STD NMR spectroscopy; aspirin silver complex; molecular docking and dynamics