Differential expression profiling of microRNAs in para-carcinoma, carcinoma and relapse human pancreatic cancer.
OBJECTIVE
To explore the altered different expression of miRNAs and the mechanisms underlying the relapse and metastasis of pancreatic cancer.


METHODS
The most differentially expressed miRNAs were analyzed by gene ontology (GO) term analysis, Kyoto encyclopedia of genes and genomes (KEGG) pathway analysis and protein interaction analysis. The potentially regulated target genes of the most differentially expressed miRNAs were also analyzed further by GO term analysis and KEGG pathway analysis, and quantitated by qRT-PCR.


RESULTS
In total, we found 12 miRNAs displayed at least a 30-fold increase or decrease in expression of carcinoma and relapse vs. para-carcinoma human pancreatic cancer (C/R vs. P). In addition, our study found that pancreatic cancer was related to pathways in cancer, including Jak-STAT signaling pathway, MAPK signaling pathway and PPAR signaling pathway.


CONCLUSIONS
The differential expressed miRNAs and their predicted target genes that involved in Jak-STAT signaling pathway, MAPK signaling pathway and PPAR signaling pathway indicating their potential roles in pancreatic carcinogenesis and progress.
Veterinary Faculty, College of Veterinary Medicine, Hunan Agricultural University, Changsha, 410128, China.
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D002277:Carcinoma / Q000235:genetics*; D015536:Down-Regulation; D015972:Gene Expression Regulation, Neoplastic; D063990:Gene Ontology; D006801:Humans; D053612:Janus Kinases / Q000235:genetics; D020935:MAP Kinase Signaling System; D035683:MicroRNAs / Q000235:genetics*; D009364:Neoplasm Recurrence, Local / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis; D010179:Pancreas / Q000737:chemistry; D010190:Pancreatic Neoplasms / Q000235:genetics*; D047492:Peroxisome Proliferator-Activated Receptors / Q000235:genetics; D050791:STAT Transcription Factors / Q000235:genetics; D059467:Transcriptome; D015854:Up-Regulation
D035683:MicroRNAs; D047492:Peroxisome Proliferator-Activated Receptors; D050791:STAT Transcription Factors; D053612:Janus Kinases