β-CateninC429S mice exhibit sterility consequent to spatiotemporally sustained Wnt signalling in the internal genitalia.
Wnt/β-catenin signalling regulates numerous developmental and homeostatic processes. Ctnnb1 (also known as β-catenin) is the only protein that transmits signals from various Wnt ligands to downstream genes. In this study, we report that our newly established mouse strain, which harbours a Cys429 to Ser missense mutation in the β-catenin gene, exhibited specific organ defects in contrast to mice with broadly functioning Wnt/β-catenin signalling. Both homozygous mutant males and females produced normal gametes but were infertile because of abnormal seminal vesicle and vaginal morphogenesis. An ins-TOPGAL transgenic reporter spatiotemporally sustained Wnt/β-catenin signalling during the corresponding organogenesis. Therefore, β-catenin(C429S) should provide new insights into β-catenin as a universal component of Wnt/β-catenin signal transduction.
Mutagenesis and Genomics Team, RIKEN BioResource Center, Tsukuba, Ibaraki, Japan.
Takuya
T
Murata
Mutagenesis and Genomics Team, RIKEN BioResource Center, Tsukuba, Ibaraki, Japan.
Yuichi
Y
Ishitsuka
Population and Quantitative Genomics Team, RIKEN Genomic Sciences Center, Yokohama, Kanagawa, Japan.
Kumiko
K
Karouji
Japan Mouse Clinic, RIKEN BioResource Center, Tsukuba, Ibaraki, Japan.
Hideki
H
Kaneda
Team for Advanced Development and Evaluation of Human Disease Models, RIKEN BioResource Center, Tsukuba, Ibaraki, Japan.
Hideaki
H
Toki
Mutagenesis and Genomics Team, RIKEN BioResource Center, Tsukuba, Ibaraki, Japan.
Yuji
Y
Nakai
Mutagenesis and Genomics Team, RIKEN BioResource Center, Tsukuba, Ibaraki, Japan.
Shigeru
S
Makino
Mutagenesis and Genomics Team, RIKEN BioResource Center, Tsukuba, Ibaraki, Japan.
Ryutaro
R
Fukumura
Mutagenesis and Genomics Team, RIKEN BioResource Center, Tsukuba, Ibaraki, Japan.
Hayato
H
Kotaki
Japan Mouse Clinic, RIKEN BioResource Center, Tsukuba, Ibaraki, Japan.
Shigeharu
S
Wakana
Team for Advanced Development and Evaluation of Human Disease Models, RIKEN BioResource Center, Tsukuba, Ibaraki, Japan.
Tetsuo
T
Noda
Mutagenesis and Genomics Team, RIKEN BioResource Center, Tsukuba, Ibaraki, Japan.
Yoichi
Y
Gondo
D000818:Animals; D004622:Embryo, Mammalian; D005260:Female; D017930:Genes, Reporter; D006720:Homozygote; D007247:Infertility, Female / Q000235:genetics*; D007248:Infertility, Male / Q000235:genetics*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D009154:Mutation; D010063:Ovum / Q000254:growth & development / Q000378:metabolism; D012669:Seminal Vesicles / Q000002:abnormalities / Q000254:growth & development / Q000378:metabolism*; D013094:Spermatozoa / Q000254:growth & development / Q000378:metabolism; D014621:Vagina / Q000002:abnormalities / Q000254:growth & development / Q000378:metabolism*; D051153:Wnt Proteins / Q000235:genetics / Q000378:metabolism; D060449:Wnt Signaling Pathway; D051176:beta Catenin / Q000235:genetics* / Q000378:metabolism
C495267:CTNNB1 protein, mouse; D051153:Wnt Proteins; D051176:beta Catenin