Spatial segmentation of MALDI FT-ICR MSI data: a powerful tool to explore the head and neck tumor in situ lipidome.
Matrix-assisted laser desorption/ionization mass spectrometric imaging (MALDI MSI) is a well-established analytical technique for determining spatial localization of lipids in biological samples. The use of Fourier-transform ion cyclotron resonance (FT-ICR) mass spectrometers for the molecular imaging of endogenous compounds is gaining popularity, since the high mass accuracy and high mass resolving power enables accurate determination of exact masses and, consequently, a more confident identification of these molecules. The high mass resolution FT-ICR imaging datasets are typically large in size. In order to analyze them in an appropriate timeframe, the following approach has been employed: the FT-ICR imaging datasets were spatially segmented by clustering all spectra by their similarity. The resulted spatial segmentation maps were compared with the histologic annotation. This approach facilitates interpretation of the full datasets by providing spatial regions of interest. The application of this approach, which has originally been developed for MALDI-TOF MSI datasets, to the lipidomic analysis of head and neck tumor tissue revealed new insights into the metabolic organization of the carcinoma tissue.
Institute of Microbiology, v.v.i., Videnska 1083, CZ 142 20, Prague 4, Czech Republic.
Lukas
L
Krasny
Franziska
F
Hoffmann
Günther
G
Ernst
Dennis
D
Trede
Theodore
T
Alexandrov
Vladimir
V
Havlicek
Orlando
O
Guntinas-Lichius
Ferdinand
F
von Eggeling
Anna C
AC
Crecelius
D005583:Fourier Analysis; D006258:Head and Neck Neoplasms / Q000737:chemistry*; D006801:Humans; D007091:Image Processing, Computer-Assisted; D008055:Lipids / Q000032:analysis* / Q000737:chemistry*; D057054:Molecular Imaging / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
D008055:Lipids