Biosynthetic incorporation of the azulene moiety in proteins with high efficiency.
Biosynthetic incorporation of β-(1-azulenyl)-L-alanine, an isostere of tryptophan, is reported using a tryptophan auxotroph expression host. The azulene moiety introduced this way in proteins features many attractive spectroscopic properties, particularly suitable for in vivo studies.
Laboratory of Biophysical Chemistry and GBB (Groningen Biomolecular Science and Biotechnology Institute), University of Groningen, Nijenborgh 7, 9747 AG, Groningen, The Netherlands.
Jinfeng
J
Shao
Ivan V
IV
Korendovych
Jaap
J
Broos
D000409:Alanine / Q000378:metabolism*; D052176:Azulenes / Q000378:metabolism*; D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000235:genetics; D013294:Lactococcus lactis / Q000235:genetics / Q000378:metabolism*; D014176:Protein Biosynthesis; D014364:Tryptophan / Q000378:metabolism
D052176:Azulenes; D001426:Bacterial Proteins; C005525:azulene; D014364:Tryptophan; D000409:Alanine