Crystallization and preliminary X-ray diffraction data of β-galactosidase from Aspergillus niger.
β-Galactosidase from Aspergillus niger (An-β-Gal), belonging to the family 35 glycoside hydrolases, hydrolyzes the β-galactosidase linkages in lactose and other galactosides. It is extensively used in industry owing to its high hydrolytic activity and safety. The enzyme has been expressed in yeasts and purified by immobilized metal-ion affinity chromatography for crystallization experiments. The recombinant An-β-Gal, deglycosylated to avoid heterogeneity of the sample, has a molecular mass of 109 kDa. Rod-shaped crystals grew using PEG 3350 as the main precipitant agent. A diffraction data set was collected to 1.8 Å resolution.
Grupo EXPRELA, Departamento de Bioloxía Celular e Molecular, Facultade de Ciencias, Universidade da Coruña, Campus da Zapateira, s/n, 15071 A Coruña, Spain.
Agustín
A
Rico-Díaz
Grupo EXPRELA, Departamento de Bioloxía Celular e Molecular, Facultade de Ciencias, Universidade da Coruña, Campus da Zapateira, s/n, 15071 A Coruña, Spain.
Ángel
Á
Vizoso Vázquez
Grupo EXPRELA, Departamento de Bioloxía Celular e Molecular, Facultade de Ciencias, Universidade da Coruña, Campus da Zapateira, s/n, 15071 A Coruña, Spain.
M Esperanza
ME
Cerdán
Grupo EXPRELA, Departamento de Bioloxía Celular e Molecular, Facultade de Ciencias, Universidade da Coruña, Campus da Zapateira, s/n, 15071 A Coruña, Spain.
Manuel
M
Becerra
Grupo de Cristalografía Macromolecular y Biología Estructural, Instituto de Química-Física Rocasolano, CSIC, Serrano 119, 28006 Madrid, Spain.
Julia
J
Sanz-Aparicio
D000595:Amino Acid Sequence; D001234:Aspergillus niger / Q000201:enzymology* / Q000235:genetics; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics; D003460:Crystallization; D030541:Databases, Genetic; D008969:Molecular Sequence Data; D014961:X-Ray Diffraction; D001616:beta-Galactosidase / Q000737:chemistry* / Q000235:genetics
D001426:Bacterial Proteins; D001616:beta-Galactosidase
Aspergillus niger; glycosidase hydrolase family 35; β-galactosidase