Efficacy of rhBMP-2 loaded PCL/PLGA/β-TCP guided bone regeneration membrane fabricated by 3D printing technology for reconstruction of calvaria defects in rabbit.
We successfully fabricated a three-dimensional (3D) printing-based PCL/PLGA/β-TCP guided bone regeneration (GBR) membrane that slowly released rhBMP-2. To impregnate the GBR membrane with intact rhBMP-2, collagen solution encapsulating rhBMP-2 (5 µg ml(-1)) was infused into pores of a PCL/PLGA/β-TCP membrane constructed using a 3D printing system with four dispensing heads. In a release profile test, sustained release of rhBMP-2 was observed for up to 28 d. To investigate the efficacy of the GBR membrane on bone regeneration, PCL/PLGA/β-TCP membranes with or without rhBMP-2 were implanted in an 8 mm calvaria defect of rabbits. Bone formation was evaluated at weeks 4 and 8 histologically and histomorphometrically. A space making ability of the GBR membrane was successfully maintained in both groups, and significantly more new bone was formed at post-implantation weeks 4 and 8 by rhBMP-2 loaded GBR membranes. Interestingly, implantation with rhBMP-2 loaded GBR membranes led to almost entire healing of calvaria defects within 8 weeks.
Department of Mechanical Engineering, Korea Polytechnic University, 237, Sangidaehak-Ro, Siheung-Si, Gyeonggi-do, 429-793, Korea.
Jin-Hyung
JH
Shim
Min-Chul
MC
Yoon
Chang-Mo
CM
Jeong
Jinah
J
Jang
Sung-In
SI
Jeong
Dong-Woo
DW
Cho
Jung-Bo
JB
Huh
D000818:Animals; D001672:Biocompatible Materials; D055396:Bone Morphogenetic Protein 2 / Q000737:chemistry*; D001861:Bone Regeneration; D001842:Bone and Bones / Q000473:pathology*; D002130:Calcium Phosphates / Q000737:chemistry*; D003094:Collagen / Q000737:chemistry; D019344:Lactic Acid / Q000737:chemistry*; D008567:Membranes, Artificial; D008855:Microscopy, Electron, Scanning; D010012:Osteogenesis; D011091:Polyesters / Q000737:chemistry*; D011100:Polyglycolic Acid / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D066330:Printing, Three-Dimensional; D011817:Rabbits; D011994:Recombinant Proteins / Q000737:chemistry; D012886:Skull / Q000187:drug effects* / Q000473:pathology
D001672:Biocompatible Materials; D055396:Bone Morphogenetic Protein 2; D002130:Calcium Phosphates; D008567:Membranes, Artificial; D011091:Polyesters; D011994:Recombinant Proteins; C485817:beta-tricalcium phosphate; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; C016240:polycaprolactone; D011100:Polyglycolic Acid; D019344:Lactic Acid; D003094:Collagen