Influence of surface-modified maghemite nanoparticles on in vitro survival of human stem cells.
Surface-modified maghemite (γ-Fe2O3) nanoparticles were obtained by using a conventional precipitation method and coated with D-mannose and poly(N,N-dimethylacrylamide). Both the initial and the modified particles were characterized by transmission electron microscopy and dynamic light scattering with regard to morphology, particle size and polydispersity. In vitro survival of human stem cells was then investigated by using the methyl thiazolyl tetrazolium (MTT) assay, which showed that D-mannose- and poly(N,N-dimethylacrylamide)-coated γ-Fe2O3 particles exhibit much lower level of cytotoxicity than the non-coated γ-Fe2O3.
Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, Heyrovského nám. 2, 162 06 Prague 6, Czech Republic.
Michal
M
Babič
Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, Heyrovského nám. 2, 162 06 Prague 6, Czech Republic.
Daniel
D
Horák
Department of Human Genetics of Institute of Molecular Biology and Genetics, NAS of Ukraine, Zabolotnogo 150, 03143 Kiev, Ukraine.
Lyubov L
LL
Lukash
Department of Human Genetics of Institute of Molecular Biology and Genetics, NAS of Ukraine, Zabolotnogo 150, 03143 Kiev, Ukraine.
Tetiana A
TA
Ruban
Department of Human Genetics of Institute of Molecular Biology and Genetics, NAS of Ukraine, Zabolotnogo 150, 03143 Kiev, Ukraine.
Yurii N
YN
Kolomiets
Department of Human Genetics of Institute of Molecular Biology and Genetics, NAS of Ukraine, Zabolotnogo 150, 03143 Kiev, Ukraine.
Svitlana P
SP
Shpylova
Department of Human Genetics of Institute of Molecular Biology and Genetics, NAS of Ukraine, Zabolotnogo 150, 03143 Kiev, Ukraine.
Oksana A
OA
Grypych
MTT assay; maghemite; magnetic; nanoparticles; stem cells