2,3-Butanedione monoxime increases sensitivity to Nikkomycin Z in the budding yeastSaccharomyces cerevisiae.
Nikkomycin Z (NZ) is a competitive inhibitor of chitin synthase III in the yeastSaccharomyces cerevisiae.Myosin type II-deficient yeast strains (myo1) display a dramatic reduction in growth when chitin synthase III activity is inhibited by NZ, supporting the contention that actomyosin motility plays an important role in maintaining cell wall integrity. A proposed inhibitor of cortical actin polymerizationin vitro, 2,3-butanedione monoxime (BDM), also inhibits growth of wild-type yeast strains at a concentration of 20 mM. In this study, we assayed for potentialin vivointerplay between BDM-sensitive cell functions and cell wall chitin synthesis by testing for increased sensitivity to NZ during co-treatment with BDM at sub-inhibitory concentrations. Our results show that BDM can increase the sensitivity of yeast cells to Nikkomycin Z.
Félix E
FE
Rivera-Molina
Sahily
S
González-Crespo
Yasdet
Y
Maldonado-De la Cruz
José M
JM
Ortiz-Betancourt
Department of Biochemistry, School of Medicine, University of Puerto Rico, P.O. Box 365067, San Juan, Puerto Rico, 00936-5067.
José R
JR
Rodríguez-Medina
BDM; Nikkomycin Z; Saccharomyces cerevisiae; chitin; myo1; yeast