Engineering lead-sensing GFP through rational designing.
Lead is one of the most hazardous metals ubiquitous in the environment, causing serious health hazards to organisms. Recently, fluorescent proteins such as GFP and Dsred were utilized for the development of reagent-less rapid metal sensors. Here, we demonstrate the development of a lead-sensing GFP that is highly sensitive to lead at micro molar concentrations.
Department of Bioscience & Biotechnology, Konkuk University, Seoul 143-701, Korea. hyungdon@konkuk.ac.kr.
Saravanan Prabhu
SP
Nadarajan
Yuvaraj
Y
Ravikumar
Kanagavel
K
Deepankumar
Chong-Soon
CS
Lee
Hyungdon
H
Yun
D001665:Binding Sites; D049452:Green Fluorescent Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007854:Lead / Q000032:analysis; D008628:Mercury / Q000032:analysis; D008670:Metals / Q000032:analysis; D015202:Protein Engineering; D013050:Spectrometry, Fluorescence
D008670:Metals; D049452:Green Fluorescent Proteins; D007854:Lead; D008628:Mercury