Physicochemical analysis of structural alteration and advanced glycation end products generation during glycation of H2A histone by 3-deoxyglucosone.
Advanced glycation end-products comprise a complex and heterogeneous group of compounds that have been implicated in diabetes-related complications. The importance of the Maillard reaction is depicted by the formation of reactive intermediate products known as α-oxoaldehydes, such as 3-deoxyglucosone (3-DG). This product has been found to be involved in accelerated vascular damage in diabetes. In the present study, calf thymus histone H2A was reacted with 3-DG, and the generation of advanced glycation end products was investigated by determining the degree of side chain modifications (lysine and arginine residues), Amadori products, carbonyl content, N(ε) -carboxymethyl lysine, and pentosidine using various physicochemical techniques. Moreover, fluorescence, absorbance as well as structural characteristics of glycated-H2A were comprehensively investigated. Overall, this study demonstrates structural perturbation, formation of different intermediates, and AGEs that are believed to hamper the normal functioning of H2A histone, compromising the integrity of chromatin structures and function in secondary complications of diabetes.
School of Biotechnology, Yeungnam University, Gyeongsan, Republic of Korea.
Jalaluddin M
JM
Ashraf
Saheem
S
Ahmad
Gulam
G
Rabbani
Arif Tasleem
AT
Jan
Eun Ju
EJ
Lee
Rizwan Hasan
RH
Khan
Inho
I
Choi
D001120:Arginine / Q000031:analogs & derivatives / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D002942:Circular Dichroism; D003847:Deoxyglucose / Q000031:analogs & derivatives* / Q000494:pharmacology; D005453:Fluorescence; D017127:Glycation End Products, Advanced / Q000737:chemistry* / Q000378:metabolism*; D006031:Glycosylation; D006657:Histones / Q000737:chemistry* / Q000378:metabolism*; D006801:Humans; D008239:Lysine / Q000031:analogs & derivatives / Q000378:metabolism; D017550:Spectroscopy, Fourier Transform Infrared
D017127:Glycation End Products, Advanced; D006657:Histones; C048496:N(6)-carboxymethyllysine; D001120:Arginine; D003847:Deoxyglucose; C062187:pentosidine; C016350:3-deoxyglucosone; D008239:Lysine
3-deoxyglucosone; Nε-carboxymethyllysine; advanced glycation end products; glycation; pentosidine