Selective capture of transcribed sequences in the functional gene analysis of microbial pathogens.
Selective capture of transcribed sequences (SCOTS) is an effective method to identify bacterial genes differentially expressed during different biological processes, including pathogenic interactions with a host species. The method can be used to elucidate molecular mechanisms driving and maintaining such interactions. The method is a powerful genetic tool that overcomes limitations found in other methods, by working with small amounts of mRNA and allowing for the separation of bacterial cDNA from host cDNA. It has been increasingly used in the discovery of genes involved in the bacterium-host interaction. In this review, we briefly introduce the SCOTS method, outline the technical advances offered in the method, and focus on the method's applications in several microbial pathogens.
College of Animal Science and Technology, Henan University of Science and Technology, Luoyang, China, wangyocean@163.com.
Yang
Y
Wang
Li
L
Yi
Shaohui
S
Wang
Chengping
C
Lu
Chan
C
Ding
D001419:Bacteria / Q000235:genetics*; D001424:Bacterial Infections / Q000382:microbiology*; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D018076:DNA, Complementary / Q000235:genetics / Q000302:isolation & purification; D015870:Gene Expression; D020869:Gene Expression Profiling; D005827:Genetics, Microbial / Q000379:methods; D054884:Host-Pathogen Interactions; D012333:RNA, Messenger / Q000235:genetics / Q000302:isolation & purification; D014158:Transcription, Genetic
D004269:DNA, Bacterial; D018076:DNA, Complementary; D012333:RNA, Messenger