Generation of iPS Cells from Granulosa Cells.
Various types of somatic cells can be reprogrammed to induced pluripotent stem (iPS) cells. Somatic stem cells may generate iPS cells more efficiently than do differentiated cells. We show that granulosa cells exhibit characteristic of somatic stem cells and can be reprogrammed to iPS cells more efficiently or with few factors. Here, we describe generation of mouse and pig iPS cells from granulosa cells with high efficiency.
State Key Laboratory of Medicinal Chemical Biology, Department of Cell Biology and Genetics, College of Life Sciences, Nankai University, 96 Weijin Road, Tianjin, 300071, China.
Jian
J
Mao
State Key Laboratory of Medicinal Chemical Biology, Department of Cell Biology and Genetics, College of Life Sciences, Nankai University, 96 Weijin Road, Tianjin, 300071, China. liulin@nankai.edu.cn.
Lin
L
Liu
D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D002478:Cells, Cultured; D065150:Cellular Reprogramming; D000067470:Cellular Reprogramming Techniques / Q000379:methods*; D015925:Cryopreservation / Q000379:methods; D003470:Culture Media / Q000494:pharmacology; D058732:Embryoid Bodies; D005260:Female; D005347:Fibroblasts / Q000166:cytology; D005822:Genetic Vectors / Q000235:genetics; D006064:Gonadotropins, Equine / Q000494:pharmacology; D006107:Granulosa Cells / Q000737:chemistry / Q000166:cytology* / Q000187:drug effects; D057809:HEK293 Cells; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology*; D034904:Lamin Type A / Q000032:analysis; D051379:Mice; D008810:Mice, Inbred C57BL; D011993:Recombinant Fusion Proteins / Q000235:genetics; D012190:Retroviridae / Q000235:genetics; D034421:Sus scrofa; D013552:Swine; D014157:Transcription Factors / Q000235:genetics
D003470:Culture Media; D006064:Gonadotropins, Equine; D034904:Lamin Type A; D011993:Recombinant Fusion Proteins; D014157:Transcription Factors
Granulosa cells; Induced pluripotent stem cells (iPSCs); Mouse; Pig; Reprogramming