Increased dipicolinic acid production with an enhanced spoVF operon in Bacillus subtilis and medium optimization.
Dipicolinic acid (DPA) is a multi-functional agent for cosmetics, antimicrobial products, detergents, and functional polymers. The aim of this study was to design a new method for producing DPA from renewable material. The Bacillus subtilis spoVF operon encodes enzymes for DPA synthase and the part of lysine biosynthetic pathway. However, DPA is only synthesized in the sporulation phase, so the productivity of DPA is low level. Here, we report that DPA synthase was expressed in vegetative cells, and DPA was produced in the culture medium by replacement of the spoVFA promoter with other highly expressed promoter in B. subtilis vegetative cells, such as spoVG promoter. DPA levels were increased in the culture medium of genetically modified strains. DPA productivity was significantly improved up to 29.14 g/L in 72 h culture by improving the medium composition using a two-step optimization technique with the Taguchi methodology.
a Biological Science Laboratories, KAO Corporation , Wakayama , Japan.
Fumikazu
F
Takahashi
Nobuyuki
N
Sumitomo
Hiroshi
H
Hagihara
Katsuya
K
Ozaki
D001412:Bacillus subtilis / Q000201:enzymology* / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D003470:Culture Media / Q000737:chemistry*; D004274:DNA, Recombinant / Q000235:genetics; D005818:Genetic Engineering; D009876:Operon / Q000235:genetics*; D010088:Oxidoreductases / Q000235:genetics* / Q000378:metabolism; D010848:Picolinic Acids / Q000378:metabolism*
D001426:Bacterial Proteins; D003470:Culture Media; D004274:DNA, Recombinant; D010848:Picolinic Acids; D010088:Oxidoreductases; C082565:dipicolinic acid synthetase, Bacillus subtilis; C004860:dipicolinic acid
Bacillus subtilis; Taguchi method; dipicolinic acid; genetically modified; medium optimization