Multiplexed digital gene expression analysis for genetical genomics in large plant populations.
Digital gene expression (DGE) analysis is a cost-effective method for large-scale quantitative transcriptome analysis using second generation sequencing. Here we describe how adaptation of DGE with barcode indexing in large segregating plant populations of over 100 genotypes can be applied for successful expression QTL (eQTL) and gene expression network analysis to develop transcript-based markers for breeding.
Department of Plant Breeding, Justus Liebig University Giessen, Heinrich-Buff-Ring 26-32, Giessen, 35392, Germany, christian.obermeier@agrar.uni-giessen.de.
Christian
C
Obermeier
Bertha M
BM
Salazar-Colqui
Viola
V
Spamer
Rod
R
Snowdon
D018076:DNA, Complementary / Q000096:biosynthesis / Q000235:genetics; D003851:Deoxyribonucleases / Q000378:metabolism; D004587:Electrophoresis, Agar Gel; D020869:Gene Expression Profiling / Q000379:methods*; D018506:Gene Expression Regulation, Plant; D023281:Genomics / Q000379:methods*; D060328:Magnetic Phenomena; D008863:Microspheres; D010944:Plants / Q000235:genetics*; D016133:Polymerase Chain Reaction; D018749:RNA, Plant / Q000302:isolation & purification; D015203:Reproducibility of Results; D015183:Restriction Mapping; D017422:Sequence Analysis, DNA; D013223:Statistics as Topic
D018076:DNA, Complementary; D018749:RNA, Plant; D003851:Deoxyribonucleases