Identification of ubiquinol binding motifs at the Qo-site of the cytochrome bc1 complex.
Enzymes of the bc1 complex family power the biosphere through their central role in respiration and photosynthesis. These enzymes couple the oxidation of quinol molecules by cytochrome c to the transfer of protons across the membrane, to generate a proton-motive force that drives ATP synthesis. Key for the function of the bc1 complex is the initial redox process that involves a bifurcated electron transfer in which the two electrons from a quinol substrate are passed to different electron acceptors in the bc1 complex. The electron transfer is coupled to proton transfer. The overall mechanism of quinol oxidation by the bc1 complex is well enough characterized to allow exploration at the atomistic level, but details are still highly controversial. The controversy stems from the uncertain binding motifs of quinol at the so-called Qo active site of the bc1 complex. Here we employ a combination of classical all atom molecular dynamics and quantum chemical calculations to reveal the binding modes of quinol at the Qo-site of the bc1 complex from Rhodobacter capsulatus. The calculations suggest a novel configuration of amino acid residues responsible for quinol binding and support a mechanism for proton-coupled electron transfer from quinol to iron-sulfur cluster through a bridging hydrogen bond from histidine that stabilizes the reaction complex.
Department of Physics, University of Illinois at Urbana-Champaign , 1110 W. Green Street, Urbana, Illinois 61801, United States.
Angela M
AM
Barragan
Antony R
AR
Crofts
Klaus
K
Schulten
Ilia A
IA
Solov'yov
D020816:Amino Acid Motifs; D004579:Electron Transport; D014450:Electron Transport Complex III / Q000737:chemistry* / Q000378:metabolism*; D056004:Molecular Dynamics Simulation; D011485:Protein Binding; D011789:Quantum Theory; D016354:Rhodobacter capsulatus / Q000201:enzymology; D014451:Ubiquinone / Q000031:analogs & derivatives* / Q000378:metabolism; D014867:Water / Q000378:metabolism
D014867:Water; D014451:Ubiquinone; D014450:Electron Transport Complex III; C003741:ubiquinol