Anti-tumor effect of cactus polysaccharides on lung squamous carcinoma cells (SK-MES-1).
BACKGROUND
Cactus polysaccharides are the active components of Opuntia dillenii which have been used extensively in folk medicine. In this study, we investigate the anti-tumor effect of cactus polysaccharides on lung squamous carcinoma cells SK-MES-1.


METHODS
The inhibitory effect of Cactus polysaccharides on lung squamous carcinoma cells were detected by MTT assay. Cell cycle was determined by flow cytometry and cell apoptosis was determined by AnnexinV assay. Western-blotting was applied to detect P53 and PTEN protein expression in the cells treated with cactus polysaccharides.


RESULTS
Results showed that different concentrations of wild cactus polysaccharides prevent SK-MES-1 cells growth and induces S phase arrest. The data also revealed that cactus polysaccharides cause apoptosis in SK-MES-1 cells determined by Annexin-V assay. Furthermore, cactus polysaccharides induced growth arrest and apoptosis may be due to the increase of P53 and phosphatase and tension homolog deleted on chromosome ten (PTEN) protein.


CONCLUSIONS
Cactus polysaccharides have anti-tumor activity on lung squamous carcinoma cells.
Department of Biotechnology, Dalian Medical University, Dalian, 116044, Liaoning, China.
W
W
Li
Department of Biotechnology, Dalian Medical University, Dalian, 116044, Liaoning, China.
D
D
Wu
Department of Biotechnology, Dalian Medical University, Dalian, 116044, Liaoning, China.
B
B
Wei
Department of Biotechnology, Dalian Medical University, Dalian, 116044, Liaoning, China.
S
S
Wang
Department of Biotechnology, Dalian Medical University, Dalian, 116044, Liaoning, China.
Hx
H
Sun
Department of Biotechnology, Dalian Medical University, Dalian, 116044, Liaoning, China.
Xl
X
Li
Department of Biotechnology, Dalian Medical University, Dalian, 116044, Liaoning, China.
F
F
Zhang
Department of Biotechnology, Dalian Medical University, Dalian, 116044, Liaoning, China.
Cl
C
Zhang
Department of Biotechnology, Dalian Medical University, Dalian, 116044, Liaoning, China.
Y
Y
Xin
D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology / Q000627:therapeutic use*; D017209:Apoptosis; D029421:Cactaceae; D002294:Carcinoma, Squamous Cell / Q000188:drug therapy*; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D006801:Humans; D008175:Lung Neoplasms / Q000188:drug therapy*; D029747:Opuntia / Q000737:chemistry*; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use*; D011134:Polysaccharides / Q000494:pharmacology / Q000627:therapeutic use*
D000972:Antineoplastic Agents, Phytogenic; D010936:Plant Extracts; D011134:Polysaccharides
Anti-tumor effect; Cactus polysaccharides; Lung squamous carcinoma; P53; PTEN