Elevated OPN, IP-10, and neutrophilia in loop-mediated isothermal amplification confirmed tuberculosis patients.
Tuberculosis (TB) is the second most common cause of death from infectious diseases and results in high socioeconomic losses to many countries. Proper diagnosis is the first step in TB eradication. To develop a rapid, simple, and accurate diagnostic TB test and to characterize the prevalence of Mycobacterium tuberculosis (MTB) genotypes and immune profiles of TB patients, a total of 37 TB patients and 30 healthy control (HC) from Metro Manila were enrolled. Loop-mediated isothermal amplification (LAMP) reliably detected MTB infection. Manila genotype was identified by spoligotyping method in all TB patients. Osteopontin (OPN), interferon-γ-induced protein 10 kDa (IP-10), and neutrophil counts were found to reflect the acute stage of MTB infection. The sensitivity and specificity were 94.6% and 93.3%, respectively, for both OPN and IP-10, and they were 83.8% and 78.6%, respectively, for neutrophils. The combination of OPN, IP-10, neutrophil count, IL-6, IL-8, TNF-α, MCP-1, platelets, galectin-9, and leukocyte count correctly identifies all the HC and 96.3% of TB patients. LAMP method may serve as a rapid, supportive method in addition to time-consuming culture methods. OPN, IP-10, and neutrophil counts are useful in detecting MTB infection and may have utility in monitoring the course of the disease.
Division of Disaster-Related Infectious Diseases, International Research Institute of Disaster Science, Tohoku University, 1-1 Seiryo-machi, Aoba-ku, Sendai, Miyagi 980-8574, Japan ; Division of Emerging Infectious Diseases, Graduate School of Medicine, Tohoku University, 1-1 Seiryo-machi, Aoba-ku, Sendai, Miyagi 980-8574, Japan ; Japan International Corporation of Welfare Services, 2-3-20 Toranomon YHK Building 4F, Toranomon, Minato-ku, Tokyo 105-0001, Japan.
Beata
0000-0001-8234-5691
B
Shiratori
STD AIDS Cooperative Central Laboratory, San Lazaro Hospital, Quiricada Street, 1003 Manila, Philippines.
Susan
S
Leano
Division of Global Epidemiology, Research Center for Zoonosis Control, Hokkaido University, North 20, West 10 Kita-ku, Sapporo 001-0020, Japan.
Chie
C
Nakajima
Division of Disaster-Related Infectious Diseases, International Research Institute of Disaster Science, Tohoku University, 1-1 Seiryo-machi, Aoba-ku, Sendai, Miyagi 980-8574, Japan ; Division of Emerging Infectious Diseases, Graduate School of Medicine, Tohoku University, 1-1 Seiryo-machi, Aoba-ku, Sendai, Miyagi 980-8574, Japan.
Haorile
0000-0003-0771-6071
H
Chagan-Yasutan
Research Division, GalPharma Company, Ltd., NEXT-Kagawa 204, 2217-44 Hayashi-cho, Takamatsu-shi, Kagawa 760-0301, Japan.
Toshiro
T
Niki
Division of Disaster-Related Infectious Diseases, International Research Institute of Disaster Science, Tohoku University, 1-1 Seiryo-machi, Aoba-ku, Sendai, Miyagi 980-8574, Japan ; Division of Emerging Infectious Diseases, Graduate School of Medicine, Tohoku University, 1-1 Seiryo-machi, Aoba-ku, Sendai, Miyagi 980-8574, Japan.
Yugo
Y
Ashino
Division of Global Epidemiology, Research Center for Zoonosis Control, Hokkaido University, North 20, West 10 Kita-ku, Sapporo 001-0020, Japan.
Yasuhiko
Y
Suzuki
STD AIDS Cooperative Central Laboratory, San Lazaro Hospital, Quiricada Street, 1003 Manila, Philippines.
Elisabeth
E
Telan
Division of Disaster-Related Infectious Diseases, International Research Institute of Disaster Science, Tohoku University, 1-1 Seiryo-machi, Aoba-ku, Sendai, Miyagi 980-8574, Japan ; Division of Emerging Infectious Diseases, Graduate School of Medicine, Tohoku University, 1-1 Seiryo-machi, Aoba-ku, Sendai, Miyagi 980-8574, Japan ; Division of Disaster-Related Infectious Diseases, International Research Institute of Disaster Science, Tohoku University, 2-1 Seiryo-machi, Aoba-ku, Sendai 980-8574, Japan.
Toshio
0000-0003-0947-7625
T
Hattori
D000293:Adolescent; D000328:Adult; D015373:Bacterial Typing Techniques; D015415:Biomarkers / Q000097:blood; D016022:Case-Control Studies; D054357:Chemokine CXCL10 / Q000097:blood*; D005260:Female; D037161:Galectins / Q000097:blood; D006801:Humans; D007958:Leukocyte Count; D008297:Male; D008875:Middle Aged; D009169:Mycobacterium tuberculosis / Q000145:classification / Q000235:genetics / Q000276:immunology; D009504:Neutrophils; D053495:Osteopontin / Q000097:blood*; D010679:Philippines; D014397:Tuberculosis, Pulmonary / Q000097:blood* / Q000175:diagnosis* / Q000382:microbiology; D055815:Young Adult
D015415:Biomarkers; C114753:CXCL10 protein, human; D054357:Chemokine CXCL10; D037161:Galectins; C105032:LGALS9 protein, human; D053495:Osteopontin