Plant regeneration of Korean wild ginseng (Panax ginseng Meyer) mutant lines induced by γ-irradiation ((60)Co) of adventitious roots.
An efficient in vitro protocol has been established for somatic embryogenesis and plantlet conversion of Korean wild ginseng (Panax ginseng Meyer). Wild-type and mutant adventitious roots derived from the ginseng produced calluses on Murashige and Skoog (MS) medium supplemented with 0.5 mg/L 2,4-dichlorophenoxyacetic acid and 0.3 mg/L kinetin; 53.3% of the explants formed callus. Embryogenic callus proliferation and somatic embryo induction occurred on MS medium containing 0.5 mg/L 2,4-dichlorophenoxyacetic acid. The induced somatic embryos further developed to maturity on MS medium with 5 mg/L gibberellic acid, and 85% of them germinated. The germinated embryos were developed to shoots and elongated on MS medium with 5 mg/L gibberellic acid. The shoots developed into plants with well-developed taproots on one-third strength Schenk and Hildebrandt basal medium supplemented with 0.25 mg/L 1-naphthaleneacetic acid. When the plants were transferred to soil, about 30% of the regenerated plants developed into normal plants.
Faculty of Biotechnology, Jeju National University, Jeju, Korea.
Jun-Ying
JY
Zhang
Subtropical Horticulture Research Institute, Jeju National University, Jeju, Korea.
Hyeon-Jin
HJ
Sun
Subtropical Horticulture Research Institute, Jeju National University, Jeju, Korea.
In-Ja
IJ
Song
Subtropical Horticulture Research Institute, Jeju National University, Jeju, Korea.
Tae-Woong
TW
Bae
Subtropical Horticulture Research Institute, Jeju National University, Jeju, Korea.
Hong-Gyu
HG
Kang
Subtropical Horticulture Research Institute, Jeju National University, Jeju, Korea.
Suk-Min
SM
Ko
Subtropical Horticulture Research Institute, Jeju National University, Jeju, Korea.
Yong-Ik
YI
Kwon
Subtropical Horticulture Research Institute, Jeju National University, Jeju, Korea.
Il-Woung
IW
Kim
School of Medicine, Jeju National University, Jeju, Korea.
Jaechun
J
Lee
Department of Clinical Pathology, Cheju Halla University, Jeju, Korea.
Shin-Young
SY
Park
Department of New Biology, Daegu Gyeongbuk Institute of Science and Technology (DGIST), Daegu, Korea.
Pyung-Ok
PO
Lim
Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry and Fisheries (IPET), Anyang, Korea.
Yong Hwan
YH
Kim
Faculty of Biotechnology, Jeju National University, Jeju, Korea ; Subtropical Horticulture Research Institute, Jeju National University, Jeju, Korea.
Hyo-Yeon
HY
Lee
Panax ginseng Meyer; mutant adventitious roots; somatic embryo; somatic embryogenesis