Label-free, isothermal and ultrasensitive electrochemical detection of DNA and DNA 3'-phosphatase using a cascade enzymatic cleavage strategy.
Label-free and ultrasensitive electrochemical assays of target DNA and T4 polynucleotide kinase phosphatase (PNKP) were developed, which took full advantage of three enzymes to realize the signal readout and amplification. It can ultimately achieve the low detection limits of 10 fM and 1 mU mL(-1) for target DNA and PNKP, respectively.
Key Laboratory of Sensor Analysis of Tumor Marker, Ministry of Education, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, China. sliu@qust.edu.cn.
Shufeng
S
Liu
Tao
T
Liu
Li
L
Wang
D004247:DNA / Q000032:analysis* / Q000378:metabolism; D015342:DNA Probes / Q000737:chemistry / Q000378:metabolism; D055664:Electrochemical Techniques; D004566:Electrodes; D005292:Ferricyanides / Q000737:chemistry; D009693:Nucleic Acid Hybridization; D009708:Nucleotidases / Q000378:metabolism*
D015342:DNA Probes; D005292:Ferricyanides; C007931:hexacyanoferrate III; D004247:DNA; D009708:Nucleotidases; C039170:deoxynucleotide 3'-phosphatase