RNA-seq approaches for determining mRNA abundance in Leishmania.
High-throughput sequencing of cDNA copies of mRNA (RNA-seq) provides a digital read-out of mRNA levels over several orders of magnitude, as well as mapping the transcripts to the nucleotide level. Here we describe an RNA-seq approach that exploits the 39-nucleotide mini-exon or spliced leader (SL) sequence found at the 5' end of all Leishmania (and other trypanosomatid) mRNAs.
Seattle BioMed, 307 Westlake Ave N., Suite 500, Seattle, WA, 98109-5219, USA.
Andrew
A
Haydock
Monica
M
Terrao
Aarthi
A
Sekar
Gowthaman
G
Ramasamy
Loren
L
Baugh
Peter J
PJ
Myler
D005786:Gene Expression Regulation; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods*; D007891:Leishmania / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods; D012333:RNA, Messenger / Q000235:genetics; D016053:RNA, Protozoan; D020038:RNA, Spliced Leader; D017423:Sequence Analysis, RNA / Q000379:methods*
D012333:RNA, Messenger; D016053:RNA, Protozoan; D020038:RNA, Spliced Leader