miR-101 suppresses tumor proliferation and migration, and induces apoptosis by targeting EZH2 in esophageal cancer cells.
OBJECTIVE
To investigate the role of miR-101 in the regulation of tumor proliferation, invasion, apoptosis and to its target gene in human ESCC.


METHODS
The expression level of miR-101 in Eca109 cell line was determined by real-time polymerase chain reaction (PCR). After transfected with miR-101 mimics and inhibitor, proliferation, migration and apoptosis in ESCC cell line (Eca109) were detected by MTT, cell wound healing assay and flow cytometry, respectively. The expression of EZH2 in Eca109 cell was examined by immunohistochemical staining.


RESULTS
We found that miR-101 was significantly down-regulated in ESCC cell than in matched normal esophageal epithelium cell. The expression level of miR-101 was inversely correlated to EZH2 protein expression in ESCC cell. In Eca109 cells, over-expression of miR-101 significantly inhibited the migration and invasion of ESCC cells, and promotes cell apoptosis.


CONCLUSIONS
These findings suggest that decreased expression of miR-101 might promote metastasis of human ESCC by inducing accumulation of EZH2 protein.
Department of Pathology, Basic Medical Sciences, Xinjiang Medical University Urumqi, China.
Chen
C
Lin
Department of Pathology, The Affiliated Tumor Hospital Of Xinjiang Medical University Urumqi, China.
Fei
F
Huang
Department of Pathology, Basic Medical Sciences, Xinjiang Medical University Urumqi, China.
Qiao-Zhi
QZ
Li
Department of Pathology, The Forth Affiliated Hospital Urumqi, China.
Ya-Jing
YJ
Zhang
D017209:Apoptosis; D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D015536:Down-Regulation; D000071221:Enhancer of Zeste Homolog 2 Protein; D004938:Esophageal Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D000077277:Esophageal Squamous Cell Carcinoma; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D009361:Neoplasm Invasiveness; D063151:Polycomb Repressive Complex 2 / Q000378:metabolism*; D015398:Signal Transduction; D013997:Time Factors; D014162:Transfection
C534072:MIRN101 microRNA, human; D035683:MicroRNAs; C498409:EZH2 protein, human; D000071221:Enhancer of Zeste Homolog 2 Protein; D063151:Polycomb Repressive Complex 2
ESCC; EZH2; MiR-101; invasion; proliferation