Cross-generational trans fat intake modifies BDNF mRNA in the hippocampus: Impact on memory loss in a mania animal model.
Recently, we have described the influence of dietary fatty acids (FA) on mania-like behavior of first generation animals. Here, two sequential generations of female rats were supplemented with soybean oil (SO, rich in n-6 FA, control group), fish oil (FO, rich in n-3 FA) and hydrogenated vegetable fat (HVF, rich in trans FA) from pregnancy and during lactation. In adulthood, half of each group was exposed to an amphetamine (AMPH)-induced mania animal model for behavioral, biochemical and molecular assessments. FO supplementation was associated with lower reactive species (RS) generation and protein carbonyl (PC) levels and increased dopamine transporter (DAT) levels, while HVF increased RS and PC levels, thus decreasing catalase (CAT) activity and DAT levels in hippocampus after AMPH treatment. AMPH impaired short- (1 h) and long- (24 h) term memory in the HVF group. AMPH exposure was able to reduce hippocampal BDNF- mRNA expression, which was increased in FO. While HVF was related to higher trans FA (TFA) incorporation in hippocampus, FO was associated with increased percentage of n-3 polyunsaturated FA (PUFA) together with lower n-6/n-3 PUFA ratio. Interestingly, our data showed a positive correlation between brain-derived neurotrophic factor (BDNF) mRNA and short- and long-term memory (r(2)  = 0.53; P = 0.000/r(2)  = 0.32; P = 0.011, respectively), as well as a negative correlation between PC and DAT levels (r(2)  = 0.23; P = 0.015). Our findings confirm that provision of n-3 or TFA during development over two generations is able to change the neuronal membrane lipid composition, protecting or impairing the hippocampus, respectively, thus affecting neurothrophic factor expression such as BDNF mRNA. In this context, chronic consumption of trans fats over two generations can facilitate the development of mania-like behavior, so leading to memory impairment and emotionality, which are related to neuropsychiatric conditions.
Programa de Pós-Graduação em Farmacologia-Universidade Federal de Santa Maria(UFSM)-RS, Brasil.
Fabíola
F
Trevizol
Verônica T
VT
Dias
Katiane
K
Roversi
Raquel C S
RC
Barcelos
Fábio T
FT
Kuhn
Karine
K
Roversi
Camila S
CS
Pase
Ronaldo
R
Golombieski
Juliana C
JC
Veit
Jaqueline
J
Piccolo
Tatiana
T
Emanuelli
João B T
JB
Rocha
Marilise E
ME
Bürger
D000818:Animals; D001714:Bipolar Disorder / Q000378:metabolism* / Q000523:psychology; D019208:Brain-Derived Neurotrophic Factor / Q000378:metabolism*; D004195:Disease Models, Animal; D050483:Dopamine Plasma Membrane Transport Proteins / Q000378:metabolism; D005260:Female; D005395:Fish Oils / Q000633:toxicity; D006624:Hippocampus / Q000378:metabolism*; D007774:Lactation; D008297:Male; D008569:Memory Disorders / Q000378:metabolism; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects; D012333:RNA, Messenger / Q000378:metabolism*; D017208:Rats, Wistar; D021641:Recognition, Psychology / Q000502:physiology; D013024:Soybean Oil / Q000633:toxicity; D044242:Trans Fatty Acids / Q000633:toxicity*; D000067075:Vegetable Products / Q000633:toxicity
D019208:Brain-Derived Neurotrophic Factor; D050483:Dopamine Plasma Membrane Transport Proteins; D005395:Fish Oils; D012333:RNA, Messenger; D044242:Trans Fatty Acids; D013024:Soybean Oil
dopamine transporter; neural membrane phospholipid; omega-3; oxidative damage