Pig skin includes dendritic cell subsets transcriptomically related to human CD1a and CD14 dendritic cells presenting different migrating behaviors and T cell activation capacities.
Swine skin is one of the best structural models for human skin, widely used to probe drug transcutaneous passage and to test new skin vaccination devices. However, little is known about its composition in immune cells, and among them dendritic cells (DC), that are essential in the initiation of the immune response. After a first seminal work describing four different DC subpopulations in pig skin, we hereafter deepen the characterization of these cells, showing the similarities between swine DC subsets and their human counterparts. Using comparative transcriptomic study, classical phenotyping as well as in vivo and in vitro functional studies, we show that swine CD163(pos) dermal DC (DDC) are transcriptomically similar to the human CD14(pos) DDC. CD163(pos) DDC are recruited in inflamed skin, they migrate in inflamed lymph but they are not attracted toward CCL21, and they modestly activate allogeneic CD8 T cells. We also show that CD163(low) DDC are transcriptomically similar to the human CD1a(pos) DDC. CD163(low) DDC migrate toward CCL21, they activate allogeneic CD8 and CD4 T cells and, like their potential human lung counterpart, they skew CD4 T cells toward a Th17 profile. We thus conclude that swine skin is a relevant model for human skin vaccination.
Virologie et Immunologie Moléculaires UR892, Institut National de la Recherche Agronomique, Domaine de Vilvert, 78352 Jouy-en-Josas, France;
Florian
F
Marquet
Centre d'Immunologie de Marseille-Luminy, UM2, Aix-Marseille Université, Case 906, 13288 Marseille Cedex 9, France; INSERM, U1104, 13288 Marseille, France; Centre National de la Recherche Scientifique, Unité Mixte de Recherche 7280, 13288 Marseille, France;
Thien-Phong
TP
Vu Manh
Virologie et Immunologie Moléculaires UR892, Institut National de la Recherche Agronomique, Domaine de Vilvert, 78352 Jouy-en-Josas, France;
Pauline
P
Maisonnasse
Virologie et Immunologie Moléculaires UR892, Institut National de la Recherche Agronomique, Domaine de Vilvert, 78352 Jouy-en-Josas, France;
Jamila
J
Elhmouzi-Younes
Virologie et Immunologie Moléculaires UR892, Institut National de la Recherche Agronomique, Domaine de Vilvert, 78352 Jouy-en-Josas, France;
Céline
C
Urien
Virologie et Immunologie Moléculaires UR892, Institut National de la Recherche Agronomique, Domaine de Vilvert, 78352 Jouy-en-Josas, France;
Edwige
E
Bouguyon
Virologie et Immunologie Moléculaires UR892, Institut National de la Recherche Agronomique, Domaine de Vilvert, 78352 Jouy-en-Josas, France;
Luc
L
Jouneau
IMAGIF, Centre National de la Recherche Scientifique, 91190 Gif-sur-Yvette, France;
Mickael
M
Bourge
Anses, Laboratoire de Ploufragan/Plouzané, Unité Virologie Immunologie Porcines, BP53, 22440 Ploufragan, France; Université Européenne de Bretagne, 35000 Rennes, France;
Gaëlle
G
Simon
Departamento de Biotecnología, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria, 28040 Madrid, Spain;
Angel
A
Ezquerra
Le Centre de Ressources Biologiques pour la Génomique des Animaux Domestiques et d'Intérêt Economique, Institut National de la Recherche Agronomique, Unité Mixte de Recherche 1313 Génétique Animale et Biologie Intégrative, F-78350 Jouy-en-Josas, France; and.
Jérôme
J
Lecardonnel
Centre de Recherche en Imagerie Interventionnelle, Institut National de la Recherche Agronomique, Domaine de Vilvert, 78352 Jouy-en-Josas, France.
Michel
M
Bonneau
Centre d'Immunologie de Marseille-Luminy, UM2, Aix-Marseille Université, Case 906, 13288 Marseille Cedex 9, France; INSERM, U1104, 13288 Marseille, France; Centre National de la Recherche Scientifique, Unité Mixte de Recherche 7280, 13288 Marseille, France;
Marc
M
Dalod
Virologie et Immunologie Moléculaires UR892, Institut National de la Recherche Agronomique, Domaine de Vilvert, 78352 Jouy-en-Josas, France;
Isabelle
I
Schwartz-Cornil
Virologie et Immunologie Moléculaires UR892, Institut National de la Recherche Agronomique, Domaine de Vilvert, 78352 Jouy-en-Josas, France; nicolas.bertho@jouy.inra.fr.
Nicolas
N
Bertho
D000818:Animals; D018949:Antigens, CD1 / Q000235:genetics / Q000378:metabolism; D000954:Antigens, Surface / Q000378:metabolism; D002633:Chemotaxis / Q000235:genetics / Q000276:immunology*; D016207:Cytokines / Q000096:biosynthesis; D003713:Dendritic Cells / Q000276:immunology* / Q000378:metabolism*; D020869:Gene Expression Profiling; D006801:Humans; D016130:Immunophenotyping; D007249:Inflammation / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D007801:Langerhans Cells / Q000276:immunology* / Q000378:metabolism*; D018950:Lipopolysaccharide Receptors / Q000235:genetics / Q000378:metabolism; D008198:Lymph Nodes / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D008213:Lymphocyte Activation / Q000276:immunology*; D008264:Macrophages / Q000276:immunology / Q000378:metabolism; D051379:Mice; D010641:Phenotype; D012867:Skin / Q000276:immunology; D013552:Swine; D016176:T-Lymphocyte Subsets / Q000276:immunology*; D059467:Transcriptome
D018949:Antigens, CD1; D000954:Antigens, Surface; C419393:CD1a antigen; D016207:Cytokines; D018950:Lipopolysaccharide Receptors