Pseudomonas aeruginosa LysR PA4203 regulator NmoR acts as a repressor of the PA4202 nmoA gene, encoding a nitronate monooxygenase.
The PA4203 gene encodes a LysR regulator and lies between the ppgL gene (PA4204), which encodes a periplasmic gluconolactonase, and, in the opposite orientation, the PA4202 (nmoA) gene, coding for a nitronate monooxygenase, and ddlA (PA4201), encoding a d-alanine alanine ligase. The intergenic regions between PA4203 and ppgL and between PA4203 and nmoA are very short (79 and 107 nucleotides, respectively). Here we show that PA4203 (nmoR) represses its own transcription and the expression of nmoA. A chromatin immunoprecipitation analysis showed the presence of a single NmoR binding site between nmoA and nmoR, which was confirmed by electrophoretic mobility shift assays (EMSAs) with the purified NmoR protein. Despite this observation, a transcriptome analysis revealed more genes to be affected in an nmoR mutant, including genes known to be part of the MexT LysR activator regulon. The PA1225 gene, encoding a quinone oxidoreductase, was the most highly upregulated gene in the nmoR deletion mutant, independently of MexT. Finally, deletion of the nmoA gene resulted in an increased sensitivity of the cells to 3-nitropropionic acid (3-NPA), confirming the role of the nitronate monooxygenase protein in the detoxification of nitronate.
Research Group of Microbiology, Department of Bioengineering Sciences, Vrije Universiteit Brussel, Brussels, Belgium VIB Department of Structural Biology, Vrije Universiteit Brussel, Brussels, Belgium.
Ken
K
Vercammen
Research Group of Microbiology, Department of Bioengineering Sciences, Vrije Universiteit Brussel, Brussels, Belgium VIB Department of Structural Biology, Vrije Universiteit Brussel, Brussels, Belgium.
Qing
Q
Wei
Research Group of Microbiology, Department of Bioengineering Sciences, Vrije Universiteit Brussel, Brussels, Belgium.
Daniel
D
Charlier
Chronic Pseudomonas Infection Research Group, Helmholtz Center for Infection Research, Braunschweig, and TWINCORE, Center for Experimental and Clinical Infection Research, Hanover, Germany.
Andreas
A
Dötsch
Chronic Pseudomonas Infection Research Group, Helmholtz Center for Infection Research, Braunschweig, and TWINCORE, Center for Experimental and Clinical Infection Research, Hanover, Germany.
Susanne
S
Haüssler
Chronic Pseudomonas Infection Research Group, Helmholtz Center for Infection Research, Braunschweig, and TWINCORE, Center for Experimental and Clinical Infection Research, Hanover, Germany.
Sebastian
S
Schulz
Department of Chemistry, Georgia State University, Atlanta, Georgia, USA.
Francesca
F
Salvi
Department of Chemistry, Georgia State University, Atlanta, Georgia, USA Department of Biology, Georgia State University, Atlanta, Georgia, USA Center for Biotechnology and Drug Design, Georgia State University, Atlanta, Georgia, USA Center for Diagnostics and Therapeutics, Georgia State University, Atlanta, Georgia, USA.
Giovanni
G
Gadda
School of Civil and Environmental Engineering, Georgia Institute of Technology, Atlanta, Georgia, USA.
Jim
J
Spain
Department of International Health, Immunology, and Microbiology, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark.
Morten Levin
ML
Rybtke
Department of International Health, Immunology, and Microbiology, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark.
Tim
T
Tolker-Nielsen
Research Group of Microbiology, Department of Bioengineering Sciences, Vrije Universiteit Brussel, Brussels, Belgium VIB Department of Structural Biology, Vrije Universiteit Brussel, Brussels, Belgium.
Jozef
J
Dingemans
Research Group of Microbiology, Department of Bioengineering Sciences, Vrije Universiteit Brussel, Brussels, Belgium VIB Department of Structural Biology, Vrije Universiteit Brussel, Brussels, Belgium.
Lumeng
L
Ye
Research Group of Microbiology, Department of Bioengineering Sciences, Vrije Universiteit Brussel, Brussels, Belgium VIB Department of Structural Biology, Vrije Universiteit Brussel, Brussels, Belgium pcornel@vub.ac.be.
Pierre
P
Cornelis
D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D003198:Computer Simulation; D021901:DNA, Intergenic / Q000235:genetics; D017353:Gene Deletion; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology*; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology*; D016680:Genome, Bacterial; D006899:Mixed Function Oxygenases / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D020411:Oligonucleotide Array Sequence Analysis; D011550:Pseudomonas aeruginosa / Q000235:genetics / Q000378:metabolism*
D001426:Bacterial Proteins; D021901:DNA, Intergenic; D006899:Mixed Function Oxygenases