Membrane-cytoskeleton dynamics in the course of mast cell activation.
Aggregation of the high-affinity IgE receptor (FcεRI) on the plasma membrane of mast cells and basophils initiates signaling events leading to a rapid release of preformed inflammatory mediators from secretory granules, and overall changes in cell morphology. Mast cell activation also causes reorganization of cytoskeletal components associated with membrane ruffling, spreading, and migration. Here we describe methods used for visualization of mast cell cytoskeleton, focusing on its two major components, microfilaments and microtubules, and their changes after cell triggering.
Laboratory of Biology of Cytoskeleton, Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, v.v.i., Vídeňská 1083, 142 20, Prague, Czech Republic, paveldra@img.cas.cz.
Pavel
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Dráber
Petr
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Dráber
D008841:Actin Cytoskeleton / Q000378:metabolism; D000818:Animals; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism*; D003599:Cytoskeleton / Q000378:metabolism*; D008407:Mast Cells / Q000166:cytology* / Q000648:ultrastructure; D051379:Mice; D008854:Microscopy, Electron; D008856:Microscopy, Fluorescence; D008870:Microtubules / Q000378:metabolism; D017455:Receptors, IgE / Q000378:metabolism; D013997:Time Factors
D017455:Receptors, IgE