Antifungal efficiency of miconazole and econazole and the interaction with transport protein: a comparative study.
BACKGROUND
Miconazole (MIZ) and econazole (ECZ) are clinically used as antifungal drugs.


OBJECTIVE
The drug effect and binding property with transport protein human serum albumin of MIZ and ECZ were studied.


METHODS
The antifungal efficiency was investigated by microdiluting drug solutions from 0 to 48 μmol L(-1) through microcalorimetry and voltammetry studies. Transmission electron microscopy was used for morphological observations of C. albicans. The interaction with HSA was studied by electrochemical methods, fluorescence spectrometry, electron microscopy, and molecular simulation.


RESULTS
IC50 of MIZ and ECZ for C. albicans were obtained as 19.72 and 29.90 μmol L(-1). Binding constants of MIZ and ECZ with HSA of 2.36 × 10(4) L mol(-1) and 3.73 × 10(4) L mol(-1) were obtained. After adding MIZ solution of 12 and 40 μmol L(-1), the peak currents increased to 4.887 and 6.024 μA. The peak currents of C. albicans in the presence of 20 and 48 μmol L(-1) ECZ were 4.701 and 5.544 μA. The docking scores for MIZ and ECZ of the best binding conformation in site I and site II were 5.60, 4.79, 5.63, and 5.85.


CONCLUSIONS
Strong inhibition to the metabolism of C. albicans and destructive effect was proved for both drugs. The lower IC50, growth rate constant of C. albicans, and higher peak current, reveal stronger antifungal activity of MIZ. Both drugs show an efficient quenching effect to intrinsic fluorescence residues of protein. MIZ mainly binds on site I while ECZ on site II. Molecular modeling experiments give further insight of the binding mechanism.
The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST KLOS) & Key Laboratory for Oral Biomedical Engineering of Ministry of Education (KLOBME), School and Hospital of Stomatology, Wuhan University , Wuhan , PR China .
Zheqin
Z
Hu
Juan
J
Zhang
Xiangrong
X
Cheng
D000935:Antifungal Agents / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology*; D002176:Candida albicans / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism / Q000648:ultrastructure; D002352:Carrier Proteins / Q000737:chemistry*; D004305:Dose-Response Relationship, Drug; D004464:Econazole / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology*; D006801:Humans; D008825:Miconazole / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology*; D046529:Microscopy, Electron, Transmission; D062105:Molecular Docking Simulation; D011485:Protein Binding; D012709:Serum Albumin / Q000737:chemistry*; D013050:Spectrometry, Fluorescence
D000935:Antifungal Agents; D002352:Carrier Proteins; D012709:Serum Albumin; D004464:Econazole; D008825:Miconazole
Azoles drug; Candida albicans, human serum albumin