A coumarin-based fluorescent probe as a central nervous system disease biomarker.
Homocysteine and methylmalonic acid are important biomarkers for diseases associated with an impaired central nervous system (CNS). A new chemoassay utilizing coumarin-based fluorescent probe 1 to detect the levels of homocysteine is successfully implemented using Parkinson's disease (PD) patients' blood serum. In addition, a rapid identification of homocysteine and methylmalonic acid levels in blood serum of PD patients was also performed using the liquid chromatography-mass spectrometry (LC-MS). The results obtained from both analyses were in agreement. The new chemoassay utilizing coumarin-based fluorescent probe 1 offers a cost- and time-effective method to identify the biomarkers in CNS patients.
Department of Chemistry, Faculty of Science, University of Malaya, Kuala Lumpur 50603, Malaysia. cady.yap@gmail.com.
Ann-Chee
AC
Yap
Department of Medicine, Faculty of Medicine, University of Malaya, Kuala Lumpur 50603, Malaysia. ummiaffah@hotmail.com.
Ummi Affah
UA
Mahamad
Department of Medicine, Faculty of Medicine, University of Malaya, Kuala Lumpur 50603, Malaysia. limshenyang@gmail.com.
Shen-Yang
SY
Lim
Department of Chemistry, Hankuk University of Foreign Studies, Yongin 449-791, Korea. haejkim@hufs.ac.kr.
Hae-Jo
HJ
Kim
Department of Chemistry, Faculty of Science, University of Malaya, Kuala Lumpur 50603, Malaysia. ymchoo@um.edu.my.
Yeun-Mun
YM
Choo
D015415:Biomarkers / Q000097:blood; D015374:Biosensing Techniques / Q000379:methods; D003374:Coumarins; D005456:Fluorescent Dyes; D006710:Homocysteine / Q000097:blood*; D006801:Humans; D008764:Methylmalonic Acid / Q000097:blood*; D010300:Parkinson Disease / Q000097:blood* / Q000175:diagnosis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods*
D015415:Biomarkers; D003374:Coumarins; D005456:Fluorescent Dyes; D006710:Homocysteine; D008764:Methylmalonic Acid; C030123:coumarin