Mycobacterium celeriflavum sp. nov., a rapidly growing scotochromogenic bacterium isolated from clinical specimens.
Six strains of a rapidly growing scotochromogenic mycobacterium were isolated from pulmonary specimens of independent patients. Biochemical and cultural tests were not suitable for their identification. The mycolic acid pattern analysed by HPLC was different from that of any other mycobacterium. Genotypic characterization, targeting seven housekeeping genes, revealed the presence of microheterogeneity in all of them. Different species were more closely related to the test strains in various regions: the type strain of Mycobacterium moriokaense showed 99.0 % 16S rRNA gene sequence similarity, and 91.5-96.5 % similarity for the remaining six regions. The whole genome sequences of the proposed type strain and that of M. moriokaense presented an average nucleotide identity (ANI) of 82.9 %. Phylogenetic analysis produced poorly robust trees in most genes with the exception of rpoB and sodA where Mycobacterium flavescens and Mycobacterium novocastrense were the closest species. This phylogenetic relatedness was confirmed by the tree inferred from five concatenated genes, which was very robust. The polyphasic characterization of the test strains, supported by the ANI value, demonstrates that they belong to a previously unreported species, for which the name Mycobacterium celeriflavum sp. nov. is proposed. The type strain is AFPC-000207(T) ( = DSM 46765(T) = JCM 18439(T)).
Department of Epidemiology, Pasteur Institute of Iran, Tehran, Iran.
Abdolrazagh Hashemi
AH
Shahraki
Department of Medical Microbiology, Ege University, Izmir, Turkey.
Cengiz
C
Çavuşoğlu
Emerging Bacterial Pathogens Unit, IRCCS San Raffaele Scientific Institute, Milan, Italy.
Emanuele
E
Borroni
Department of Bacteriology and Virology, Alborz University of Medical Science, Karaj, Iran.
Parvin
P
Heidarieh
Institute for Medical Experimental Research, Istanbul University, Istanbul, Turkey.
Orhan Kaya
OK
Koksalan
Emerging Bacterial Pathogens Unit, IRCCS San Raffaele Scientific Institute, Milan, Italy.
Andrea Maurizio
AM
Cabibbe
Infectious and Tropical Diseases Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
Mohamad
M
Hashemzadeh
Diagnostic Genetics Unit, Careggi University Hospital, Florence, Italy.
Alessandro
A
Mariottini
Department of Epidemiology, Pasteur Institute of Iran, Tehran, Iran.
Ehsan
E
Mostafavi
Center for Translational Genomics and Bioinformatics, IRCCS San Raffaele Scientific Institute, Milan, Italy.
Davide
D
Cittaro
Department of Microbiology, Tehran University of Medical Science, Tehran, Iran.
Mohamad Mehdi
MM
Feizabadi
Center for Translational Genomics and Bioinformatics, IRCCS San Raffaele Scientific Institute, Milan, Italy.
Dejan
D
Lazarevic
Department of Epidemiology, Pasteur Institute of Iran, Tehran, Iran.
Farhad
F
Yaghmaei
Microbiology Laboratory, Ospedale Maggiore Carità, Novara, Italy.
Gian Lorenzo
GL
Molinari
Microbiology Laboratory, Ospedale Maggiore Carità, Novara, Italy.
Anna
A
Camaggi
Emerging Bacterial Pathogens Unit, IRCCS San Raffaele Scientific Institute, Milan, Italy.
Enrico
E
Tortoli
D000328:Adult; D000368:Aged; D015373:Bacterial Typing Techniques; D002675:Child, Preschool; D004269:DNA, Bacterial / Q000235:genetics; D005260:Female; D005798:Genes, Bacterial; D006801:Humans; D007492:Iran; D008297:Male; D008826:Microbial Sensitivity Tests; D008875:Middle Aged; D008969:Molecular Sequence Data; D009161:Mycobacterium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D009164:Mycobacterium Infections / Q000382:microbiology*; D009171:Mycolic Acids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D014421:Turkey
D004269:DNA, Bacterial; D009171:Mycolic Acids; D012336:RNA, Ribosomal, 16S