Visualizing mammalian brain area interactions by dual-axis two-photon calcium imaging.
Fluorescence Ca(2+) imaging enables large-scale recordings of neural activity, but collective dynamics across mammalian brain regions are generally inaccessible within single fields of view. Here we introduce a two-photon microscope possessing two articulated arms that can simultaneously image two brain areas (∼0.38 mm(2) each), either nearby or distal, using microendoscopes. Concurrent Ca(2+) imaging of ∼100-300 neurons in primary visual cortex (V1) and lateromedial (LM) visual area in behaving mice revealed that the variability in LM neurons' visual responses was strongly dependent on that in V1, suggesting that fluctuations in sensory responses propagate through extended cortical networks.
James H. Clark Center for Biomedical Engineering &Sciences, Stanford University, Stanford, California, USA.
Jérôme
J
Lecoq
1] James H. Clark Center for Biomedical Engineering &Sciences, Stanford University, Stanford, California, USA. [2] Howard Hughes Medical Institute, Stanford University, Stanford, California, USA.
Joan
J
Savall
James H. Clark Center for Biomedical Engineering &Sciences, Stanford University, Stanford, California, USA.
Dejan
D
Vučinić
James H. Clark Center for Biomedical Engineering &Sciences, Stanford University, Stanford, California, USA.
Benjamin F
BF
Grewe
James H. Clark Center for Biomedical Engineering &Sciences, Stanford University, Stanford, California, USA.
Hyun
H
Kim
James H. Clark Center for Biomedical Engineering &Sciences, Stanford University, Stanford, California, USA.
Jin Zhong
JZ
Li
James H. Clark Center for Biomedical Engineering &Sciences, Stanford University, Stanford, California, USA.
Lacey J
LJ
Kitch
1] James H. Clark Center for Biomedical Engineering &Sciences, Stanford University, Stanford, California, USA. [2] Howard Hughes Medical Institute, Stanford University, Stanford, California, USA. [3] CNC Program, Stanford University, Stanford, California, USA.
Mark J
MJ
Schnitzer
D000200:Action Potentials / Q000502:physiology*; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D005260:Female; D008297:Male; D051379:Mice; D057507:Mice, 129 Strain; D008810:Mice, Inbred C57BL; D036641:Microscopy, Fluorescence, Multiphoton / Q000379:methods*; D010775:Photic Stimulation / Q000379:methods*; D014793:Visual Cortex / Q000737:chemistry / Q000502:physiology*; D014795:Visual Pathways / Q000737:chemistry / Q000502:physiology
D002118:Calcium