Expression analysis of several antiviral related genes to BmNPV in different resistant strains of silkworm, Bombyx mori.
Bombyx mori L. (Lepidoptera: Bombycidae) nucleopolyhedrovirus (BmNPV) is a highly pathogenic virus in the sericultural industry, often causing severe damage leading to large economic losses. The immune mechanisms of B. mori against this virus remain obscure. Previous studies had demonstrated Bmlipase-1, BmNox and Bmserine protease-2 showing antiviral activity in vitro, but data on the transcription levels of these proteins in different resistant strains were not reported. In order to determine the resistance level of the four different strains (P50, A35, A40, A53) and gain a better understanding of the mechanism of resistance to BmNPV in B. mori, the relative expression level of the genes coding the three antiviral proteins in larval haemolymph and midgut of different B. mori strains resistant to BmNPV was determined. The results showed that these genes expressed significantly higher in the resistant strains compared to the susceptible strain, and the differential expression levels were consistent with the LC50 values in different strains. The transcription level of the target genes almost all up-regulated in the larvae midgut and down-regulated in the haemolymph. The results indicate the correlation of these genes to BmNPV resistance in B. mori.
School of Life Sciences, Anhui Agricultural University, Hefei 230036, China youngchengmail@126.com.
Yang
Y
Cheng
School of Life Sciences, Anhui Agricultural University, Hefei 230036, China 738471791@qq.com.
Xue-yang
XY
Wang
School of Life Sciences, Anhui Agricultural University, Hefei 230036, China 13dc@163.com.
Chang
C
Du
School of Life Sciences, Anhui Agricultural University, Hefei 230036, China haust-gaojuan@163.com.
Juan
J
Gao
School of Life Sciences, Anhui Agricultural University, Hefei 230036, China jiapingxu@163.com.
Jia-ping
JP
Xu
D000818:Animals; D012831:Bombyx / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D041981:Gastrointestinal Tract / Q000378:metabolism; D006458:Hemolymph / Q000378:metabolism; D054884:Host-Pathogen Interactions / Q000235:genetics*; D017924:Nucleopolyhedroviruses / Q000502:physiology*; D060888:Real-Time Polymerase Chain Reaction
BmNox; Bmlipase-1; Bmserine protease-2; Bombyx mori nucleopolyhedrovirus; expression analysis