Micro+nanotexturing of substrates to enhance ligand-assisted cancer cell isolation.
This paper presents a simple approach to create a two-tiered surface for superior cancer cell isolation. The idea is inspired by the interactions of cells with a nanotextured basement membrane. The texture mimicked the extracellular matrix and basement membrane for superior target cell adhesion. Prepared micro+nanotextured surfaces showed enhanced cell capture. Preparation of the two-tiered surface was done using micro- and nanotexturing and was easily reproducible. It has been shown before that the larger surface area of a nanotextured surface assists the cell's attachment through surface-anchored ligands. Taking it a step further, ligand functionalized two-level micro+nanotextured surfaces improved the sensitivity of the cancer cell isolation over simple flat nanotexturing. The isolation efficiency increased by 208% compared to the surface with a single-level nanotexture. The two-tiered surface was compatible with previously reported nanotextured devices used for cancer cell isolation. Micro-texture on the glass surface was created using simple sand gritting, followed by reactive ion etching (RIE) of the entire surface. The approach could create large surface areas within a short time while maintaining superior cell isolation efficiency.
Nano-Bio Lab, University of Texas at Arlington, Texas, USA. Department of Electrical Engineering, University of Texas at Arlington, Texas, USA. Nanotechnology Research Center, Shimadzu Institute for Research Technologies, University of Texas at Arlington, Texas, USA.
Mohammed Arif I
MA
Mahmood
Yuan
Y
Wan
Muhymin
M
Islam
Waqas
W
Ali
Madiha
M
Hanif
Young-tae
YT
Kim
Samir M
SM
Iqbal
D001485:Basement Membrane; D002448:Cell Adhesion; D002469:Cell Separation; D005109:Extracellular Matrix; D005909:Glioblastoma / Q000175:diagnosis*; D006801:Humans; D008024:Ligands; D049329:Nanostructures / Q000737:chemistry*
D008024:Ligands