Cancer-associated fibroblasts are not formed from cancer cells by epithelial-to-mesenchymal transition in nu/nu mice.
Cancer-associated fibroblasts are bioactive elements influencing the biological properties of malignant tumors. Their origin from different cell types has been established, and the possibility of their formation by epithelial-to-mesenchymal transition from cancer cells is under debate. This study shows that human cancer cells grafted to nu/nu mice induced formation of tumor stroma with the presence of typical smooth muscle actin-containing cancer-associated fibroblasts. These cells seem to be of the host origin because they are not recognized by an antibody specific for human vimentin, as was also verified in vitro. These results suggest that cancer-associated stromal fibroblasts are not formed by epithelial-to-mesenchymal transition from cancer cells.
First Faculty of Medicine, Institute of Anatomy, Charles University, U nemocnice 3, 128 00, Prague 2, Czech Republic.
Barbora
B
Dvořánková
Karel
K
Smetana
Blanka
B
Říhová
Jan
J
Kučera
Rosana
R
Mateu
Pavol
P
Szabo
D000230:Adenocarcinoma / Q000378:metabolism / Q000473:pathology*; D000818:Animals; D014408:Biomarkers, Tumor / Q000378:metabolism; D002294:Carcinoma, Squamous Cell / Q000378:metabolism / Q000473:pathology*; D019070:Cell Lineage; D015179:Colorectal Neoplasms / Q000378:metabolism / Q000473:pathology*; D058750:Epithelial-Mesenchymal Transition; D005260:Female; D005347:Fibroblasts / Q000378:metabolism / Q000473:pathology*; D019073:HT29 Cells; D006258:Head and Neck Neoplasms / Q000378:metabolism / Q000473:pathology*; D064593:Heterografts; D006801:Humans; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D010610:Pharyngeal Neoplasms / Q000378:metabolism / Q000473:pathology*; D000077195:Squamous Cell Carcinoma of Head and Neck; D017154:Stromal Cells / Q000378:metabolism / Q000473:pathology*; D013997:Time Factors
D014408:Biomarkers, Tumor