Induced Pluripotent Stem Cells: Generation, Characterization, and Differentiation--Methods and Protocols.
Reprogramming fibroblasts into induced pluripotent stem cells (iPSC) remains a promising technique for cell replacement therapy. Diverse populations of somatic cells have been examined for their reprogramming potential. Recently, ocular ciliary body epithelial cells (CECs) have been reprogrammed with high reprogramming efficiency and single transcription factor reprogramming, making them an exciting candidate for cellular reprogramming strategies.
Department of Surgery, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.
Veronica Kon
VK
Graversen
Department of Ophthalmology, University of North Carolina, 5151 Bioinformatics Building, CB #7040, Chapel Hill, NC, 27599-7040, USA. schavala@med.unc.edu.
Sai H
SH
Chavala
D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation; D002478:Cells, Cultured; D065150:Cellular Reprogramming / Q000187:drug effects; D000067470:Cellular Reprogramming Techniques / Q000379:methods*; D002924:Ciliary Body / Q000166:cytology*; D003470:Culture Media / Q000494:pharmacology; D004847:Epithelial Cells / Q000166:cytology; D005822:Genetic Vectors / Q000235:genetics; D057026:Induced Pluripotent Stem Cells / Q000166:cytology* / Q000187:drug effects; D016086:Lentivirus / Q000235:genetics; D051379:Mice; D011993:Recombinant Fusion Proteins / Q000235:genetics; D018874:Spheroids, Cellular; D014157:Transcription Factors / Q000235:genetics / Q000502:physiology
D003470:Culture Media; D011993:Recombinant Fusion Proteins; D014157:Transcription Factors
Ciliary body epithelial cells; Induced pluripotent stem cells; Reprogramming; Somatic cells; Transcription factors