Biological Evaluation of a New Synthesized Pt(II) Complex by Cytotoxic and Spectroscopic Studies.
In this study, the interaction of β-casein (β-CN) with a new synthesized Pt(II) complex (bipyridin morpholin dithiocarbamate Pt(II) nitrate), as an anticancer compound, was studied. This study was carried by fluorescence and circular dichroism (CD) measurements at 25 and 37° C. Also, cytotoxicity and apoptotic activity of the complex were studied against cancer model cell line of K562 using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Fluorescence data revealed that the intrinsic fluorescence of β-CN was quenched by the addition of Pt(II) complex through dynamic quenching mechanism. The CD spectra indicated that the binding of Pt(II) complex to β-CN causes a slight conformational change in the secondary structure of protein. Also, MTT assay represented growth inhibitory effect of the complex toward the cancer cell line. From above results, it can be concluded that β-CN can bind to the Pt complex and transfer this new anticancer drug. It may be suggested that the antitumor activity of this complex against K562 cell reveals typical morphology features of apoptotic death.
Department of Cell & Molecular Biology, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran. divsalar@khu.ac.ir.
Adeleh
A
Divsalar
Department of Cell & Molecular Biology, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran.
Mahdieh
M
Razmi
Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran.
Ali Akbar
AA
Saboury
Department of Chemistry, University of Sistan & Baluchestan, Zahedan, Iran.
Hassan
H
Mansouri-Torshizi
Centre for Interdisciplinary Research in Basic Sciences, Jamia Millia Islamia, New Delhi, India.
Faizan
F
Ahmad
D000970:Antineoplastic Agents / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D002364:Caseins / Q000378:metabolism; D002942:Circular Dichroism; D004354:Drug Screening Assays, Antitumor; D006801:Humans; D020014:K562 Cells; D009944:Organoplatinum Compounds / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology*; D013050:Spectrometry, Fluorescence; D013816:Thermodynamics
C000589196:(bipyridine)(morpholine dithiocarbamate)platinum(II); D000970:Antineoplastic Agents; D002364:Caseins; D009944:Organoplatinum Compounds
Anticancer; Apoptosis; Pt(II) complex; Quenching; β-Casein