Cell growth on pore-graded biomimetic TiO2 bone scaffolds.
In order to prevent soft tissue down-growth into osseous defect areas, membranes are used when placing bone graft materials. These membranes still show shortcomings in their performance and applications. In the current study, we choose an approach to integrate micro-porous surface structures into a macro-porous scaffold. Low porous surfaces were fabricated by dip-coatings. Four different material compositions (titanium dioxide, polycaprolactone, polycaprolactone/water, polycaprolactone/β-tricalcium phosphate) were characterised in terms of their appearance, architecture, topographical features and cell response. Titanium dioxide surfaces exhibited rougher and more complex textures, resulting in the highest number of osteosarcoma cells and distinct morphologies in terms of cell spreading. Polycaprolactone-based surfaces showed a smoother topography and enhanced microporosity, but the effect on secretion of the bone markers sclerostin and interleukin-6 from human osteoblasts was lower compared to secretion from cells cultured on titanium dioxide. β-Tricalcium phosphate modification of polycaprolactone did not show any significant improvement regarding cell-material interaction. Nevertheless, surfaces show potential in the mechanical blockage of epithelial and soft tissue cells and may still permit sufficient nutrient transport.
Department of Biomaterials, University of Oslo, Norway.
Benjamin
B
Müller
Department of Biomaterials, University of Oslo, Norway.
Janne Elin
JE
Reseland
Department of Biomaterials, University of Oslo, Norway.
Håvard Jostein
HJ
Haugen
Department of Biomaterials, University of Oslo, Norway hanna.tiainen@odont.uio.no.
Hanna
H
Tiainen
D048868:Adaptor Proteins, Signal Transducing; D040761:Biomimetic Materials / Q000737:chemistry*; D019485:Bone Morphogenetic Proteins / Q000378:metabolism; D018786:Bone Substitutes / Q000737:chemistry; D002130:Calcium Phosphates / Q000737:chemistry; D002460:Cell Line; D049109:Cell Proliferation; D020099:Coated Materials, Biocompatible / Q000737:chemistry; D005819:Genetic Markers; D006801:Humans; D015850:Interleukin-6 / Q000378:metabolism; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D010006:Osteoblasts / Q000166:cytology / Q000378:metabolism; D011091:Polyesters / Q000737:chemistry; D016062:Porosity; D013499:Surface Properties; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry*; D014025:Titanium / Q000737:chemistry*
D048868:Adaptor Proteins, Signal Transducing; D019485:Bone Morphogenetic Proteins; D018786:Bone Substitutes; D002130:Calcium Phosphates; D020099:Coated Materials, Biocompatible; D005819:Genetic Markers; C508600:IL6 protein, human; D015850:Interleukin-6; D011091:Polyesters; C422903:SOST protein, human; C485817:beta-tricalcium phosphate; C009495:titanium dioxide; C016240:polycaprolactone; D014025:Titanium
TiO2; dip-coating; micro-porous surface; osteoblast; polycaprolactone; pore structure; scaffold; soft tissue