Light-emitting diode therapy in exercise-trained mice increases muscle performance, cytochrome c oxidase activity, ATP and cell proliferation.
Light-emitting diode therapy (LEDT) applied over the leg, gluteus and lower-back muscles of mice using a LED cluster (630 nm and 850 nm, 80 mW/cm(2) , 7.2 J/cm(2) ) increased muscle performance (repetitive climbing of a ladder carrying a water-filled tube attached to the tail), ATP and mitochondrial metabolism; oxidative stress and proliferative myocyte markers in mice subjected to acute and progressive strength training. Six bi-daily training sessions LEDT-After and LEDT-Before-After regimens more than doubled muscle performance and increased ATP more than tenfold. The effectiveness of LEDT on improving muscle performance and recovery suggest applicability for high performance sports and in training programs. Positioning of the mice and light-emitting diode therapy (LEDT) applied on mouse legs, gluteus and lower-back muscles without contact.
Laboratory of Electrothermophototherapy, Department of Physical Therapy, Federal University of Sao Carlos, SP, Brazil.|Post-Graduation Program in Biotechnology, Federal University of Sao Carlos, SP, Brazil.|Optics Group, Physics Institute of Sao Carlos, University of São Paulo, Sao Carlos, SP, Brazil.|Wellman Center for Photomedicine, Massachusetts General Hospital, 40 Blossom Street, Boston, MA 02114, USA.
Cleber
C
Ferraresi
Laboratory of Electrothermophototherapy, Department of Physical Therapy, Federal University of Sao Carlos, SP, Brazil.|Post-Graduation Program in Biotechnology, Federal University of Sao Carlos, SP, Brazil.
Nivaldo Antonio
NA
Parizotto
Wellman Center for Photomedicine, Massachusetts General Hospital, 40 Blossom Street, Boston, MA 02114, USA.|Laboratory of Radiation Dosimetry and Medical Physics, Institute of Physics, Sao Paulo University,, SP, Brazil.
Marcelo Victor
MV
Pires de Sousa
Wellman Center for Photomedicine, Massachusetts General Hospital, 40 Blossom Street, Boston, MA 02114, USA.|Federal University of Rio de Janeiro, RJ, Brazil.
Beatriz
B
Kaippert
Wellman Center for Photomedicine, Massachusetts General Hospital, 40 Blossom Street, Boston, MA 02114, USA.|Department of Dermatology, Harvard Medical School, Boston, MA, USA.
Ying-Ying
YY
Huang
Wellman Center for Photomedicine, Massachusetts General Hospital, 40 Blossom Street, Boston, MA 02114, USA.|Graduate School Biomedical Engineering, Tohoku University, Japan.
Tomoharu
T
Koiso
Optics Group, Physics Institute of Sao Carlos, University of São Paulo, Sao Carlos, SP, Brazil.
Vanderlei Salvador
VS
Bagnato
Wellman Center for Photomedicine, Massachusetts General Hospital, 40 Blossom Street, Boston, MA 02114, USA. mhamblin@partners.org.|Department of Dermatology, Harvard Medical School, Boston, MA, USA. mhamblin@partners.org.|Harvard-MIT Division of Health Science and Technology, Cambridge, MA, USA. mhamblin@partners.org.
Michael R
MR
Hamblin
D000255:Adenosine Triphosphate / Q000378:metabolism*; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D002374:Catalase / Q000378:metabolism; D049109:Cell Proliferation / Q000528:radiation effects; D003576:Electron Transport Complex IV / Q000378:metabolism*; D006003:Glycogen / Q000378:metabolism; D015227:Lipid Peroxidation / Q000528:radiation effects; D028022:Low-Level Light Therapy; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D053580:Muscle Strength / Q000528:radiation effects; D009132:Muscles / Q000166:cytology / Q000378:metabolism / Q000502:physiology* / Q000528:radiation effects*; D018384:Oxidative Stress / Q000528:radiation effects; D010805:Physical Conditioning, Animal; D050050:Protein Carbonylation / Q000528:radiation effects; D016474:Weight-Bearing
D015415:Biomarkers; D000255:Adenosine Triphosphate; D006003:Glycogen; D002374:Catalase; D003576:Electron Transport Complex IV
ATP; LEDT; LLLT; muscle performance; muscle recovery