Multi-isotype antibody responses against the multimeric Salmonella Typhi recombinant hemolysin E antigen.
The detection and measurement of different antibody isotypes in the serum provide valuable indicators of the different stages of typhoid infection. Here, the ability of S. Typhi recombinant hemolysin E (HlyE) to detect multi-isotype antibody responses in sera of patients with typhoid and paratyphoid A was investigated using an indirect antibody immunoassay. Nanogram amounts of HlyE were found to be sufficient for detection of IgG and IgA isotypes and, in a study of individuals' sera (n = 100), the immunoassay was able to distinguish between typhoid and non-typhoid sera. The overall sensitivity, specificity and efficiency of the ELISA were 70% (39/56), 100% (44/44) and 83% respectively.
Institute for Research in Molecular Medicine, Universiti Sains Malaysia, 11800, Minden, Malaysia.
Eugene Boon Beng
EB
Ong
Joshua
J
Ignatius
Amy Amilda
AA
Anthony
Ismail
I
Aziah
Asma
A
Ismail
Theam Soon
TS
Lim
D000907:Antibodies, Bacterial / Q000097:blood*; D000942:Antigens, Bacterial / Q000235:genetics / Q000276:immunology*; D003955:Diagnostic Tests, Routine / Q000379:methods*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006460:Hemolysin Proteins / Q000235:genetics / Q000276:immunology*; D006513:Hepatitis B e Antigens; D006801:Humans; D007070:Immunoglobulin A / Q000097:blood; D007074:Immunoglobulin G / Q000097:blood; D010284:Paratyphoid Fever / Q000175:diagnosis; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology; D012485:Salmonella typhi / Q000235:genetics / Q000276:immunology*; D012680:Sensitivity and Specificity; D012698:Serologic Tests / Q000379:methods; D014435:Typhoid Fever / Q000175:diagnosis*
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D006460:Hemolysin Proteins; D006513:Hepatitis B e Antigens; C531350:HlyE protein, Salmonella enterica; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D011994:Recombinant Proteins
ELISA; Salmonella Typhi; antibody isotypes; recombinant hemolysin E antigen