Proteomic Profiling of a Biomimetic Drug Delivery Platform.
Current delivery platforms are typically designed for prolonged circulation that favors superior accumulation of the payload in the targeted tissue. The design of efficient surface modifications determines both a longer circulation time and targeting abilities of particles. The optimization of synthesis protocols to efficiently combine targeting molecules and elements that allow for an increased circulation time can be challenging and almost impossible when several functional elements are needed. On the other hand, in the last decade, the development of bioinspired technologies was proposed as a new approach with which to increase particle safety, biocompatibility and targeting, while maintaining the synthesis protocols simple and reproducible. Recently, we developed a new drug delivery system inspired by the biology of immune cells called leukolike vector (LLV) and formed by a nanoporous silicon core and a shell derived from the leucocyte cell membrane. The goal of this study is to investigate the protein content of the LLV. Here we report the proteomic profiling of the LLV and demonstrate that our approach can be used to modify the surface of synthetic particles with more than 150 leukocyte membrane associated proteins that determine particle safety, circulation time and targeting abilities towards inflamed endothelium.
Claudia
C
Corbo
Alessandro
A
Parodi
Michael
M
Evangelopoulos
David A
DA
Engler
Rise K
RK
Matsunami
Anthony C
AC
Engler
Roberto
R
Molinaro
Shilpa
S
Scaria
Francesco
F
Salvatore
Department of Nanomedicine, The Houston Methodist Research Institute, Houston, 6670 Bertner Avenue, Houston Texas 77030, USA. etasciotti@HoustonMethodist.org.
Ennio
E
Tasciotti
D000818:Animals; D032701:Biomimetics / Q000379:methods*; D002460:Cell Line; D002462:Cell Membrane / Q000737:chemistry; D016503:Drug Delivery Systems; D007962:Leukocytes / Q000737:chemistry; D051379:Mice; D053758:Nanoparticles; D016062:Porosity; D011506:Proteins / Q000737:chemistry; D040901:Proteomics / Q000379:methods*; D012825:Silicon / Q000737:chemistry
D011506:Proteins; D012825:Silicon