Modulation of rotation-induced lift force for cell filtration in a low aspect ratio microchannel.
Cell filtration is a critical step in sample preparation in many bioapplications. Herein, we report on a simple, filter-free, microfluidic platform based on hydrodynamic inertial migration. Our approach builds on the concept of two-stage inertial migration which permits precise prediction of microparticle position within the microchannel. Our design manipulates equilibrium positions of larger microparticles by modulating rotation-induced lift force in a low aspect ratio microchannel. Here, we demonstrate filtration of microparticles with extreme efficiency (>99%). Using multiple prostate cell lines (LNCaP and human prostate epithelial tumor cells), we show filtration from spiked blood, with 3-fold concentration and >83% viability. Results of a proliferation assay show normal cell division and suggest no negative effects on intrinsic properties. Considering the planar low-aspect-ratio structure and predictable focusing, we envision promising applications and easy integration with existing lab-on-a-chip systems.
BioMicroSystems Lab, Department of Electrical Engineering and Computing Systems, University of Cincinnati , Cincinnati, Ohio 45221, USA.
Jian
J
Zhou
Department of Environmental Health, College of Medicine, University of Cincinnati , Cincinnati, Ohio 45221, USA.
Premkumar Vummidi
PV
Giridhar
Department of Environmental Health, College of Medicine, University of Cincinnati , Cincinnati, Ohio 45221, USA.
Susan
S
Kasper
BioMicroSystems Lab, Department of Electrical Engineering and Computing Systems, University of Cincinnati , Cincinnati, Ohio 45221, USA.
Ian
I
Papautsky