Targeting the proteome of cellular fractions: focus on secreted proteins.
The high complexity of the total cellular proteome underscores the need for a more targeted investigation of particular subcellular fractions as a means to detect the changes at the level of low abundance proteins. However, this approach requires the application of an enrichment strategy. In this chapter, we present the protocols, which have been used for the analysis of secretome from cell lines, targeting the investigation of protein expression changes.
Biotechnology Division, Biomedical Research Foundation, Academy of Athens, Soranou Ephessiou 4, 11527, Athens, Greece.
Agnieszka
A
Latosinska
Maria
M
Frantzi
William
W
Mullen
Antonia
A
Vlahou
Manousos
M
Makridakis
D002460:Cell Line; D017077:Culture Media, Conditioned; D004721:Endoplasmic Reticulum / Q000378:metabolism; D006056:Golgi Apparatus / Q000378:metabolism; D020543:Proteome / Q000302:isolation & purification / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*
D017077:Culture Media, Conditioned; D020543:Proteome