Using the ubiquitous pH meter combined with a loop mediated isothermal amplification method for facile and sensitive detection of Nosema bombycis genomic DNA PTP1.
Here we show an amplification-coupled detection method for directly measuring released hydrogen ions during the loop mediated isothermal amplification (LAMP) procedure by using a pH meter. The genomic DNA of Nosema bombycis (N. bombycis) was amplified and detected by employing this LAMP-pH meter platform for the first time.
State Key Laboratory of Silkworm Genome Biology, Key Laboratory of Luminescent and Real-Time Analytical Chemistry, Ministry of Education (Southwest University), College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, PR China. yuanruo@swu.edu.cn yqchai@swu.edu.cn.
Shunbi
S
Xie
Yali
Y
Yuan
Yue
Y
Song
Ying
Y
Zhuo
Tian
T
Li
Yaqin
Y
Chai
Ruo
R
Yuan
D017931:DNA Primers / Q000737:chemistry / Q000378:metabolism; D004271:DNA, Fungal / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D016818:Nosema / Q000235:genetics*; D021141:Nucleic Acid Amplification Techniques
D017931:DNA Primers; D004271:DNA, Fungal