Survival of skin graft between transgenic cloned dogs and non-transgenic cloned dogs.
Whereas it has been assumed that genetically modified tissues or cells derived from somatic cell nuclear transfer (SCNT) should be accepted by a host of the same species, their immune compatibility has not been extensively explored. To identify acceptance of SCNT-derived cells or tissues, skin grafts were performed between cloned dogs that were identical except for their mitochondrial DNA (mtDNA) haplotypes and foreign gene. We showed here that differences in mtDNA haplotypes and genetic modification did not elicit immune responses in these dogs: 1) skin tissues from genetically-modified cloned dogs were successfully transplanted into genetically-modified cloned dogs with different mtDNA haplotype under three successive grafts over 63 days; and 2) non-transgenic cloned tissues were accepted into transgenic cloned syngeneic recipients with different mtDNA haplotypes and vice versa under two successive grafts over 63 days. In addition, expression of the inserted gene was maintained, being functional without eliciting graft rejection. In conclusion, these results show that transplanting genetically-modified tissues into normal, syngeneic or genetically-modified recipient dogs with different mtDNA haplotypes do not elicit skin graft rejection or affect expression of the inserted gene. Therefore, therapeutically valuable tissue derived from SCNT with genetic modification might be used safely in clinical applications for patients with diseased tissues.
Department of Theriogenology & Biotechnology, College of Veterinary Medicine, Seoul National University, Seoul, Republic of Korea.
Geon A
GA
Kim
Department of Theriogenology & Biotechnology, College of Veterinary Medicine, Seoul National University, Seoul, Republic of Korea.
Hyun Ju
HJ
Oh
Department of Theriogenology & Biotechnology, College of Veterinary Medicine, Seoul National University, Seoul, Republic of Korea.
Min Jung
MJ
Kim
Department of Theriogenology & Biotechnology, College of Veterinary Medicine, Seoul National University, Seoul, Republic of Korea.
Young Kwang
YK
Jo
Department of Theriogenology & Biotechnology, College of Veterinary Medicine, Seoul National University, Seoul, Republic of Korea.
Jin
J
Choi
Department of Theriogenology & Biotechnology, College of Veterinary Medicine, Seoul National University, Seoul, Republic of Korea.
Jung Eun
JE
Park
Department of Theriogenology & Biotechnology, College of Veterinary Medicine, Seoul National University, Seoul, Republic of Korea.
Eun Jung
EJ
Park
Central Research Institutes, K-stem cell, Seoul, Republic of Korea.
Sang Hyun
SH
Lim
Laboratory of Histology and Molecular Pathogenesis, College of Veterinary Medicine, Kangwon National University, Chuncheon, Gangwon-do, Republic of Korea.
Byung Il
BI
Yoon
Central Research Institutes, K-stem cell, Seoul, Republic of Korea.
Sung Keun
SK
Kang
Department of Theriogenology & Biotechnology, College of Veterinary Medicine, Seoul National University, Seoul, Republic of Korea.
Goo
G
Jang
Department of Theriogenology & Biotechnology, College of Veterinary Medicine, Seoul National University, Seoul, Republic of Korea.
Byeong Chun
BC
Lee
D000818:Animals; D030801:Animals, Genetically Modified; D004272:DNA, Mitochondrial / Q000235:genetics; D004285:Dogs; D005260:Female; D006239:Haplotypes; D064596:Isografts / Q000276:immunology / Q000637:transplantation*; D053652:Nuclear Transfer Techniques; D012867:Skin / Q000276:immunology*; D016038:Skin Transplantation / Q000379:methods*
D004272:DNA, Mitochondrial