Designer self-assembling hydrogel scaffolds can impact skin cell proliferation and migration.
There is a need to develop economical, efficient and widely available therapeutic approaches to enhance the rate of skin wound healing. The optimal outcome of wound healing is restoration to the pre-wound quality of health. In this study we investigate the cellular response to biological stimuli using functionalized nanofibers from the self-assembling peptide, RADA16. We demonstrate that adding different functional motifs to the RADA16 base peptide can influence the rate of proliferation and migration of keratinocytes and dermal fibroblasts. Relative to unmodified RADA16; the Collagen I motif significantly promotes cell migration, and reduces proliferation.
School of Chemistry and Biochemistry, M313, The University of Western Australia, 35 Stirling Highway, Crawley, WA, 6009 (Australia).
Michael
M
Bradshaw
School of Chemistry and Biochemistry, M313, The University of Western Australia, 35 Stirling Highway, Crawley, WA, 6009 (Australia).
Diwei
D
Ho
Fiona Wood Foundation and Burn Injury Research Unit, M318, The University of Western Australia, 35 Stirling Highway, Crawley, WA, 6009 (Australia).
Mark W
MW
Fear
1] Center for Nanomedicine and Tissue Engineering, CNTE - 4th floor Pad. Mariani, A.O., Ospedale Niguarda Ca' Granda, Piazza dell'Ospedale Maggiore 3, Milan, 20162 (Italy) [2] IRCCS Casa Sollievo della Sofferenza Opera di San Pio da Pietrelcina, San Giovanni Rotondo 71013 (Italy).
Fabrizio
F
Gelain
1] Fiona Wood Foundation and Burn Injury Research Unit, M318, The University of Western Australia, 35 Stirling Highway, Crawley, WA, 6009 (Australia) [2] Burns Service of Western Australia, Royal Perth and Princess Margaret Hospitals, WA, 6000 (Australia).
Fiona M
FM
Wood
School of Chemistry and Biochemistry, M313, The University of Western Australia, 35 Stirling Highway, Crawley, WA, 6009 (Australia).
K Swaminathan
KS
Iyer
D000818:Animals; D002460:Cell Line; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D006801:Humans; D020100:Hydrogels / Q000737:chemistry / Q000494:pharmacology*; D015603:Keratinocytes / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D051379:Mice; D008954:Models, Biological; D041681:NIH 3T3 Cells; D057139:Nanofibers / Q000737:chemistry / Q000648:ultrastructure; D010455:Peptides / Q000138:chemical synthesis / Q000494:pharmacology*; D013329:Structure-Activity Relationship; D054457:Tissue Scaffolds; D014945:Wound Healing / Q000502:physiology
D020100:Hydrogels; D010455:Peptides