House dust mite induced lung inflammation does not alter circulating vitamin D levels.
Low circulating levels of 25-hydroxyvitamin D [25(OH)D] are associated with chronic lung diseases such as asthma. However, it is unclear whether vitamin D is involved in disease pathogenesis or is modified by the inflammation associated with the disease process. We hypothesized that allergic inflammation decreases the level of circulating 25(OH)D and tested this using a mice model of house dust mite (HDM) induced allergic airway inflammation. Cellular influx was measured in bronchoalvelar lavage (BAL) fluid, and allergic sensitization and 25(OH)D levels were measured in serum. Exposure to HDM caused a robust inflammatory response in the lung that was enhanced by prior influenza infection. These responses were not associated with any change in circulating levels of 25(OH)D. These data suggest that alterations in circulating 25(OH)D levels induced by Th-2 driven inflammation are unlikely to explain the cross-sectional epidemiological association between vitamin D deficiency and asthma.
School of Medicine, Faculty of Health, University of Tasmania, Hobart, Tasmania, Australia.
Ling
L
Chen
Harry Perkins Institute of Medical Research, Nedlands, WA, Australia.
Kara L
KL
Perks
Telethon Kids Institute, University of Western Australia, Subiaco, WA, Australia.
Stephen M
SM
Stick
Telethon Kids Institute, University of Western Australia, Subiaco, WA, Australia.
Anthony
A
Kicic
Telethon Kids Institute, University of Western Australia, Subiaco, WA, Australia.
Alexander N
AN
Larcombe
School of Medicine, Faculty of Health, University of Tasmania, Hobart, Tasmania, Australia.
Graeme
G
Zosky
D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry / Q000166:cytology; D004804:Eosinophils / Q000276:immunology / Q000473:pathology / Q000821:virology; D005260:Female; D007073:Immunoglobulin E / Q000097:blood; D007249:Inflammation / Q000097:blood* / Q000209:etiology / Q000276:immunology / Q000821:virology; D009980:Influenza A virus / Q000276:immunology; D008168:Lung / Q000276:immunology / Q000473:pathology / Q000821:virology; D008264:Macrophages / Q000276:immunology / Q000473:pathology / Q000821:virology; D051379:Mice; D008807:Mice, Inbred BALB C; D009504:Neutrophils / Q000276:immunology / Q000473:pathology / Q000821:virology; D009976:Orthomyxoviridae Infections / Q000097:blood* / Q000276:immunology / Q000473:pathology / Q000821:virology; D039981:Pyroglyphidae / Q000276:immunology*; D012130:Respiratory Hypersensitivity / Q000097:blood* / Q000209:etiology / Q000276:immunology / Q000821:virology; D018418:Th2 Cells / Q000276:immunology / Q000473:pathology / Q000821:virology; D014807:Vitamin D / Q000031:analogs & derivatives* / Q000097:blood
D014807:Vitamin D; D007073:Immunoglobulin E; C104450:25-hydroxyvitamin D