Phenolic composition, antioxidant capacity of Salvia verticcilata and effect on multidrug resistant bacteria by flow-cytometry.
BACKGROUND
Antioxidants are of great importance for preventing oxidative stress that may cause several degenerative diseases. Studies have indicated phytochemicals have high free-radical scavenging activity, which helps to reduce the risk of chronic diseases. The aim of the present study is the determination of antioxidant properties, polyphenolic content and multidrug resistant bacteria of Salvia verticcilata L.


METHODS
Methanol was used as the extraction solvent. The total phenolic content was calculated using Folin-Ciocalteau method and phenolic composition was determined by HPLC. The radical scavenging activity of plant was evaluated in vitro based on the reduction of the stable DPPH free radical. The reducing capacity was identified by using the FRAP method. The ability of Salvia verticcilata L. to increase the permeability of multidrug resistant bacterial cells was conducted by flow cytometric assay on Listeria innocua and E-coli.


RESULTS
The amount of total phenolics was found to be 347.5 mg GA/g extract. The IC50 value and FRAP assay are 0.61, and 0.944 respectively, Free radical scavenging effect and FRAP values are less than synthetic antioxidant compounds (BHA and BHT). Eight phenolic compounds were found in Salvia verticcilata L. Intense concentration of S. verticcilata L. has destroyed 97 % of living cells for Listeria innocua and 94.86% for E-coli.


CONCLUSIONS
This study shows that methanolic extracts of Salvia verticcilata L. is a potential source of natural antioxidants and antimicrobial agent and can form the basis for pharmacological studies.
Mustafa Kemal University, Chemistry Dept.., Hatay Turkey.
Yener
Y
Tekeli
Mustafa Kemal University, Chemistry Dept.., Hatay Turkey.
Esra
E
Karpuz
Mustafa Kemal University, Chemistry Dept.., Hatay Turkey.
Hatice
H
Danahaliloglu
Mustafa Kemal University, Chemistry Dept.., Hatay Turkey.
Serbay
S
Bucak
Mustafa Kemal University, Biology Dept., Hatay Turkey.
Yelda
Y
Guzel
University of Applied Sciences, Flensburg Germany.
Helmuth
H
Erdmann
D000900:Anti-Bacterial Agents / Q000032:analysis / Q000494:pharmacology*; D000975:Antioxidants / Q000032:analysis / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects*; D001713:Biphenyl Compounds / Q000378:metabolism; D018432:Drug Resistance, Multiple / Q000187:drug effects*; D004926:Escherichia coli / Q000187:drug effects; D005434:Flow Cytometry / Q000379:methods; D020128:Inhibitory Concentration 50; D008087:Listeria / Q000187:drug effects; D010851:Picrates / Q000378:metabolism; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D059808:Polyphenols / Q000032:analysis / Q000494:pharmacology*; D027544:Salvia / Q000737:chemistry*
D000900:Anti-Bacterial Agents; D000975:Antioxidants; D001713:Biphenyl Compounds; D010851:Picrates; D010936:Plant Extracts; D059808:Polyphenols; C004931:1,1-diphenyl-2-picrylhydrazyl
Salvia verticcilata; antioxidant; medicinal plant; multidrug resistant; phenolic composition