Distinct expression profiles of three melatonin receptors during early development and metamorphosis in the flatfish Solea senegalensis.
Melatonin actions are mediated through G protein-coupled transmembrane receptors. Recently, mt1, mt2, and mel1c melatonin receptors were cloned in the Senegalese sole. Here, their day-night and developmental expressions were analyzed by quantitative PCR. These results revealed distinct expression patterns of each receptor through development. mel1c transcripts were more abundant in unfertilized ovulated oocytes and declined during embryonic development. mt1 and mt2 expression was higher at the earliest stages (2-6 days post-fertilization), decreasing before (mt2) or during (mt1) metamorphosis. Only mt1 and mel1c expression exhibited day-night variations, with higher nocturnal mRNA levels. These results suggest different roles and transcriptional regulation of these melatonin receptors during flatfish development and metamorphosis.
Department of Biology, Faculty of Marine and Environmental Sciences, University of Cádiz, Marine Campus of International Excellence (CEIMAR), Agrifood Campus of International Excellence (ceiA3), E-11510 Puerto Real, Spain. olivier@iats.csic.es.
Olivier
O
Lan-Chow-Wing
Department of Biology, Faculty of Marine and Environmental Sciences, University of Cádiz, Marine Campus of International Excellence (CEIMAR), Agrifood Campus of International Excellence (ceiA3), E-11510 Puerto Real, Spain. francesca.confente@uca.es.
Francesca
F
Confente
Department of Biology, Faculty of Marine and Environmental Sciences, University of Cádiz, Marine Campus of International Excellence (CEIMAR), Agrifood Campus of International Excellence (ceiA3), E-11510 Puerto Real, Spain. patricia.herrera@uca.es.
Patricia
P
Herrera-Pérez
Department of Physiology (Animal Physiology II), Faculty of Biology, Complutense University of Madrid, E-28040 Madrid, Spain. eisornaa@bio.ucm.es.
Esther
E
Isorna
IEO, Spanish Institute of Oceanography, Santander Oceanographic Centre, Promontorio de San Martín, s/n, P.O. Box 240, E-39080 Santander, Spain. o.chereguini@st.ieo.es.
Olvido
O
Chereguini
Department of Biology, Faculty of Marine and Environmental Sciences, University of Cádiz, Marine Campus of International Excellence (CEIMAR), Agrifood Campus of International Excellence (ceiA3), E-11510 Puerto Real, Spain. maricarmen.rendon@uca.es.
Maria del Carmen
Mdel C
Rendón
Aragó Laboratory-UMR7628 (CNRS and UPMC) and GDR2821 (CNRS/Ifremer), F-66651 Banyuls/Mer, France. falcon@obs-banyuls.fr.
Jack
J
Falcón
Department of Biology, Faculty of Marine and Environmental Sciences, University of Cádiz, Marine Campus of International Excellence (CEIMAR), Agrifood Campus of International Excellence (ceiA3), E-11510 Puerto Real, Spain. munoz.cueto@uca.es.
José A
JA
Muñoz-Cueto
D000818:Animals; D029941:Fish Proteins / Q000235:genetics*; D005412:Flatfishes / Q000196:embryology / Q000235:genetics* / Q000254:growth & development*; D018507:Gene Expression Regulation, Developmental; D008675:Metamorphosis, Biological; D044094:Receptors, Melatonin / Q000235:genetics*
D029941:Fish Proteins; D044094:Receptors, Melatonin