An airborne actinobacteriaNocardiopsis albaisolated from bioaerosol of a mushroom compost facility.
Actinobacteriaare widely distributed in many environments and represent the most important trigger to the occupant respiratory health. Health complaints, including hypersensitivity pneumonitis of the workers, were recorded in a mushroom compost facility (MCF). The studies on the airborne bacteria were carried out to find a possible microbiological source of these symptoms. Culture analysis of compost bioaerosols collected in different location of the MCF was performed. An assessment of the indoor microbial exposure revealed bacterial flora of bioaerosol in the mushroom compost facility represented byBacillus,Geobacillus,Micrococcus,Pseudomonas,Staphylococcusspp., and actinobacterial strain with white aerial mycelium. The thermotolerant actinobacterial strain of the same morphology was repeatedly isolated from many locations in MCF: air, compost sample, and solid surface in production hall. On the base of complex morphological, chemotaxonomic, and phylogenetic characteristics, the isolate has been classified asNocardiopsis alba. Dominant position ofN. albain microbial environment of the mushroom compost facility may represent an indicator microorganism in compost bioaerosol. The bioavailability ofN. albain mushroom compost facility creates potential risk for the health of workers, and the protection of respiratory tract and/or skin is strongly recommended.
Laboratory of Medical Microbiology, Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Rudolfa Weigla 12, 53-114 Wrocław, Poland.
Mariola
M
Paściak
Laboratory of Molecular Biology of Microorganisms, Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Rudolfa Weigla 12, 53-114 Wrocław, Poland.
Krzysztof
K
Pawlik
Laboratory of Medical Microbiology, Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Rudolfa Weigla 12, 53-114 Wrocław, Poland.
Andrzej
A
Gamian
Laboratory of Medical Microbiology, Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Rudolfa Weigla 12, 53-114 Wrocław, Poland.
Bogumiła
B
Szponar
Institute of Fermentation Technology and Microbiology, Technical University of Łódź, Wólczańska 171/173, 90-924 Lodz, Poland.
Justyna
J
Skóra
Institute of Fermentation Technology and Microbiology, Technical University of Łódź, Wólczańska 171/173, 90-924 Lodz, Poland.
Beata
B
Gutarowska
Actinobacteria; Airborne microorganisms; Bioaerosol; Compost; Indoor microbial exposure; Nocardiopsis alba; Occupational exposure