Oxidative stress markers and C-reactive protein are related to severity of heart failure in patients with dilated cardiomyopathy.
BACKGROUND
The aim of study was to determine relationships between functional capacity (NYHA class), left ventricle ejection fraction (LVEF), hemodynamic parameters, and biomarkers of redox state and inflammation in patients with dilated cardiomyopathy (DCM).


METHODS
DCM patients (n = 109, aged 45.97 ± 10.82 years), NYHA class IIV, and LVEF 2.94 ± 7.1% were studied. Controls comprised age-matched healthy volunteers (n = 28). Echocardiography and right heart catheterization were performed. Serum activities of superoxide dismutase isoenzymes (MnSOD and CuZnSOD), concentrations of uric acid (UA), malondialdehyde (MDA), and C-reactive protein (hs-CRP) were measured.


RESULTS
MnSOD, UA, hs-CRP, and MDA were significantly higher in DCM patients compared to controls. Except MDA concentration, above parameters were higher in patients in III-IV NYHA class or with lower LVEF. hsCRP correlated with of MnSOD (P < 0.05) and CuZnSOD activity (P < 0.01). Both isoenzymes positively correlated with mPAP and pulmonary capillary wedge pressure (MnSOD, resp., P < 0.01 and P < 0.05 and CuZnSOD P < 0.05; P < 0.05). UA positively correlated with MnSOD (P < 0.05), mPAP (P < 0.05), and PVRI (P < 0.05). The negative correlation between LVEF and UA (P < 0.01) was detected.


CONCLUSIONS
There are relationships among the severity of symptoms of heart failure, echocardiographic hemodynamic parameters, oxidative stress, and inflammatory activation. Increased MnSOD activity indicates the mitochondrial source of ROS in patients with advanced heart failure.
Second Department of Cardiology, School of Medicine with the Division of Dentistry, Medical University of Silesia, M. C. Skłodowskiej 10 Street, 41-800 Zabrze, Poland.
Celina
0000-0003-3700-8439
C
Wojciechowska
Department of Biochemistry, School of Medicine with the Division of Dentistry, Medical University of Silesia, Jordana 19 Street, 41-808 Zabrze, Poland.
Ewa
0000-0002-2555-8102
E
Romuk
Second Department of Cardiology, School of Medicine with the Division of Dentistry, Medical University of Silesia, M. C. Skłodowskiej 10 Street, 41-800 Zabrze, Poland.
Andrzej
0000-0002-9312-762X
A
Tomasik
Department of Biochemistry, School of Medicine with the Division of Dentistry, Medical University of Silesia, Jordana 19 Street, 41-808 Zabrze, Poland.
Bronisława
B
Skrzep-Poloczek
Second Department of Cardiology, School of Medicine with the Division of Dentistry, Medical University of Silesia, M. C. Skłodowskiej 10 Street, 41-800 Zabrze, Poland.
Ewa
E
Nowalany-Kozielska
Department of Biochemistry, School of Medicine with the Division of Dentistry, Medical University of Silesia, Jordana 19 Street, 41-808 Zabrze, Poland.
Ewa
E
Birkner
Second Department of Cardiology, School of Medicine with the Division of Dentistry, Medical University of Silesia, M. C. Skłodowskiej 10 Street, 41-800 Zabrze, Poland.
Wojciech
W
Jacheć
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015415:Biomarkers / Q000097:blood; D002097:C-Reactive Protein / Q000378:metabolism*; D002311:Cardiomyopathy, Dilated / Q000097:blood* / Q000503:physiopathology; D016022:Case-Control Studies; D005260:Female; D006333:Heart Failure / Q000097:blood* / Q000503:physiopathology; D006439:Hemodynamics; D006801:Humans; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D008875:Middle Aged; D018384:Oxidative Stress; D011669:Pulmonary Wedge Pressure; D013318:Stroke Volume; D013482:Superoxide Dismutase / Q000097:blood; D014527:Uric Acid / Q000097:blood; D016277:Ventricular Function, Left; D055815:Young Adult
D015415:Biomarkers; D014527:Uric Acid; D008315:Malondialdehyde; D002097:C-Reactive Protein; D013482:Superoxide Dismutase