In vitro method of studying the angiotensin activity of chemical compounds.
We developed and tested an experimental model to study in vitro the type 1 angiotensin antagonistic activity of compounds on the isolated portal vein of rats. The reliability of this method was confirmed in tests with saralasin (nonselective antagonist of angiotensin receptors) and losartan (selective antagonist of type 1 angiotensin receptors) in concentrations of 10(-9)-10(-5) mol/liter. The half-maximal inhibitory concentrations (IC50) of these substances were calculated.
Volgograd State Medical University, Ministry of Health of the Russian Federation; Volgograd Medical Scientific Center, Volgograd, Russia.
A A
AA
Spasov
D S
DS
Yakovlev
T M
TM
Bukatina
A A
AA
Brigadirova
D047228:Angiotensin II Type 1 Receptor Blockers / Q000494:pharmacology*; D000818:Animals; D004353:Drug Evaluation, Preclinical; D005260:Female; D066298:In Vitro Techniques; D020128:Inhibitory Concentration 50; D019808:Losartan / Q000494:pharmacology*; D008297:Male; D009119:Muscle Contraction / Q000187:drug effects; D009131:Muscle, Smooth, Vascular / Q000187:drug effects; D011169:Portal Vein / Q000187:drug effects / Q000502:physiology; D017208:Rats, Wistar; D012504:Saralasin / Q000494:pharmacology*; D014661:Vasoconstriction / Q000187:drug effects
D047228:Angiotensin II Type 1 Receptor Blockers; D012504:Saralasin; D019808:Losartan