Crystallization and preliminary X-ray analysis of the hypothetical deaminase RPB_0146 from Rhodopseudomonas palustris HaA2.
RPB_0146, a putative deaminase from Rhodopseudomonas palustris HaA2, was expressed in Escherichia coli BL21 (DE3) cells and purified using a His6 tag by Ni2+-chelating affinity chromatography for X-ray crystallographic analysis. Diffraction-quality crystals were grown by the hanging-drop vapour-diffusion method at 289 K and diffracted to a resolution of 2.44 Å using a wavelength of 1.000 Å at the Photon Factory (KEK), Japan. The crystals belonged to the orthorhombic space group P2₁2₁2₁, with unit-cell parameters a=66.26, b=123.94, c=155.95 Å.
Tianjin University of Science and Technology, Tianjin 300222, People's Republic of China.
Guofang
G
Zhang
Tianjin University of Science and Technology, Tianjin 300222, People's Republic of China.
Dan
D
Yu
Tianjin International Joint Academy of Biotechnology and Medicine, Tianjin 300457, People's Republic of China.
Guodong
G
Yang
Tianjin International Joint Academy of Biotechnology and Medicine, Tianjin 300457, People's Republic of China.
Hui
H
Dong
Tianjin University of Science and Technology, Tianjin 300222, People's Republic of China.
Tongcun
T
Zhang
Tianjin International Joint Academy of Biotechnology and Medicine, Tianjin 300457, People's Republic of China.
Xiang
X
Liu
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics; D003460:Crystallization; D018360:Crystallography, X-Ray; D008969:Molecular Sequence Data; D012241:Rhodopseudomonas / Q000201:enzymology* / Q000235:genetics
D001426:Bacterial Proteins
RPB_0146; Rhodopseudomonas palustris HaA2; deaminase