Sphingobium subterraneum sp. nov., isolated from ground water.
A Gram-stain-negative, strictly aerobic, non-motile, non-spore-forming, yellow, rod-shaped bacterium, designated S-II-13(T), was isolated from ground water at Daejeon in Korea. Strain S-II-13(T) grew between 15 and 30 °C (optimal growth at 28 °C), between pH 6.0 and 9.0 (optimal growth at pH 7.5) and at salinities of 0.3-1.5 % (w/v) NaCl, growing optimally with 0.5 % (w/v) NaCl. On the basis of 16S rRNA gene sequence analysis, strain S-II-13(T) was found to belong to the genus Sphingobium, showing closest phylogenetic similarity to Rhizorhapis suberifaciens CA1(T) (97.0 % 16S rRNA gene sequence similarity), Sphingobium sufflavum HL-25(T) (96.9 %) and Sphingobium vulgare HU1-GD12(T) (96.6 %). The major polar lipids were phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylmonomethylethanolamine, phosphatidylcholine and sphingoglycolipid. The predominant ubiquinone was Q-10. The major fatty acids were C18 : 1ω7c, C14 : 0 2-OH, C16 : 0 and summed feature 3 (C16 : 1ω7c and/or iso-C15 : 0 2-OH). The DNA G+C content of strain S-II-13(T) was 63.5 mol%. DNA-DNA relatedness between strain S-II-13(T) and Rhizorhapis suberifaciens LMG 17323(T), Sphingobium sufflavum KCTC 23953(T) and Sphingobium vulgare KCTC 22289(T) was 24, 52 and 55 %, respectively. On the basis of evidence from this taxonomic study using a polyphasic approach, strain S-II-13(T) represents a novel species of the genus Sphingobium for which the name Sphingobium subterraneum sp. nov. is proposed. The type strain is S-II-13(T) ( = KACC 17606(T) = NBRC 109814(T)).
Institute of Microbial Ecology and Resources, Mokwon University, 88 Doanbuk-ro, Seo-gu, Daejeon 302-318, Republic of Korea.
Jae-Chan
JC
Lee
University of Science and Technology, Daejeon 305-850, Republic of Korea.|Microbial Resource Center/KCTC, Korea Research Institute of Bioscience and Biotechnology, Daejeon 305-806, Republic of Korea.
Song-Gun
SG
Kim
Department of Microbial & Nano Materials, College of Science & Technology, Mokwon University, 88 Doanbuk-ro, Seo-gu, Daejeon 302-318, Republic of Korea.|Institute of Microbial Ecology and Resources, Mokwon University, 88 Doanbuk-ro, Seo-gu, Daejeon 302-318, Republic of Korea.
Kyung-Sook
KS
Whang
D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D060587:Groundwater / Q000382:microbiology*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D010858:Pigmentation; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D017422:Sequence Analysis, DNA; D042301:Sphingomonadaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D014451:Ubiquinone / Q000031:analogs & derivatives / Q000737:chemistry
D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D014451:Ubiquinone; C024989:coenzyme Q10