A chromatin modifying enzyme, SDG8, is involved in morphological, gene expression, and epigenetic responses to mechanical stimulation.
Thigmomorphogenesis is viewed as being a response process of acclimation to short repetitive bursts of mechanical stimulation or touch. The underlying molecular mechanisms that coordinate changes in how touch signals lead to long-term morphological changes are enigmatic. Touch responsive gene expression is rapid and transient, and no transcription factor or DNA regulatory motif has been reported that could confer a genome wide mechanical stimulus. We report here on a chromatin modifying enzyme, SDG8/ASHH2, which can regulate the expression of many touch responsive genes identified in Arabidopsis. SDG8 is required for the permissive expression of touch induced genes; and the loss of function of sdg8 perturbs the maximum levels of induction on selected touch gene targets. SDG8 is required to maintain permissive H3K4 trimethylation marks surrounding the Arabidopsis touch-inducible gene TOUCH 3 (TCH3), which encodes a calmodulin-like protein (CML12). The gene neighboring was also slightly down regulated, revealing a new target for SDG8 mediated chromatin modification. Finally, sdg8 mutants show perturbed morphological response to wind-agitated mechanical stimuli, implicating an epigenetic memory-forming process in the acclimation response of thigmomorphogenesis.
Hawkesbury Institute for the Environment, University of Western Sydney Penrith, NSW, Australia ; Australian Research Council Centre of Excellence in Plant Energy Biology, Research School of Biology, College of Medicine, Biology and Environment, The Australian National University Canberra, ACT, Australia.
Christopher I
CI
Cazzonelli
Australian Research Council Centre of Excellence in Plant Energy Biology, Research School of Biology, College of Medicine, Biology and Environment, The Australian National University Canberra, ACT, Australia.
Nazia
N
Nisar
Australian Research Council Centre of Excellence in Plant Energy Biology, Research School of Biology, College of Medicine, Biology and Environment, The Australian National University Canberra, ACT, Australia.
Andrea C
AC
Roberts
Australian Research Council Centre of Excellence in Plant Energy Biology, Research School of Biology, College of Medicine, Biology and Environment, The Australian National University Canberra, ACT, Australia.
Kevin D
KD
Murray
Australian Research Council Centre of Excellence in Plant Energy Biology, Research School of Biology, College of Medicine, Biology and Environment, The Australian National University Canberra, ACT, Australia.
Justin O
JO
Borevitz
Australian Research Council Centre of Excellence in Plant Energy Biology, Research School of Biology, College of Medicine, Biology and Environment, The Australian National University Canberra, ACT, Australia.
Barry J
BJ
Pogson
Arabidopsis; acclimation; histone; mechanical; regulation; thigmomorphogenesis; touch; transcription