Involvement of Toll-like receptors in cervical cancer susceptibility among Tunisian women.
Previous studies underscored the importance of genetic factors in the pathogenesis of certain cancers, including cervical cancer. Epidemiological evidence supports an association between specific polymorphisms of Toll-like receptors (TLR) with several human pathological states, including cervical cancer. The aim of this study was to investigate the link between specific gene variants in TLR2 (-196 to -174 del), TLR3 (c.1377 C>T), TLR4 (Asp299Gly), and TLR9 (2848 G>A) and susceptibility to cervical cancer in Tunisian women. Study subjects comprised 122 women with histopathologically-confirmed cervical cancer, and 260 unrelated age- and ethnically-matched healthy females, who served as controls. TLR genotyping was done using PCR-restriction fragment length polymorphism. The C/C genotype of TLR3 (c.1377 C>T) is associated with cervical cancer susceptibility (OR: 1.71, CI: 1.08-2.70). For TLR4 (Asp299Gly), the Asp/Asp genotype and the Asp allele were associated with higher risk of developing cervical cancer (OR: 4.95, CI: 1.97-13.22) and (OR: 5.17, CI: 2.11-13.50) respectively. We demonstrated no association between the TLR2 (-196 to -174 del) and the TLR 9 (2848 G>A) polymorphisms and the susceptibility of cervical cancer among Tunisian women. However, the C/C genotype for the TLR3 (c.1377 C>T) polymorphism and the Asp/Asp genotype and the Asp allele for (Asp299Gly) TLR4 polymorphism were found to be associated with a higher risk of cervical cancer.
Université de Tunis El Manar, Faculté des Sciences de Tunis, LR 206 Micro-Organismes et Bio-molécules Actives, 1092 El Manar I, Tunis, Tunisia.
Sabrina
S
Zidi
Baskent university, Faculty of medicine, Department of Medical Biology, Ankara, Turkey.
Hasibe
H
Verdi
Baskent university, Faculty of medicine, Department of Medical Biology, Ankara, Turkey.
Yaprak
Y
Yilmaz-Yalcin
Baskent university, Faculty of medicine, Department of Biostatistics, Ankara, Turkey.
A C
AC
Yazici
Military Hospital of Tunis, Laboratory of Immunology, Tunis, Tunisia.
Ezzedine
E
Gazouani
Salah Azaiz Oncology Institute, boulevard 9 avril 1938, 1006 Tunis, Tunisia.
Amel
A
Mezlini
Baskent university, Faculty of medicine, Department of Medical Biology, Ankara, Turkey.
Fatma-Belgin
FB
Atac
Université de Tunis El Manar, Faculté des Sciences de Tunis, LR 206 Micro-Organismes et Bio-molécules Actives, 1092 El Manar I, Tunis, Tunisia.
Besma
B
Yacoubi-Loueslati
D000230:Adenocarcinoma / Q000235:genetics / Q000473:pathology; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000483:Alleles; D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000473:pathology; D016022:Case-Control Studies; D016001:Confidence Intervals; D005260:Female; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D008875:Middle Aged; D016017:Odds Ratio; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D012044:Regression Analysis; D012189:Retrospective Studies; D012306:Risk; D012509:Sarcoma / Q000235:genetics / Q000473:pathology; D051195:Toll-Like Receptor 2 / Q000235:genetics*; D051196:Toll-Like Receptor 3 / Q000235:genetics*; D051197:Toll-Like Receptor 4 / Q000235:genetics*; D051217:Toll-Like Receptor 9 / Q000235:genetics*; D014416:Tunisia; D002583:Uterine Cervical Neoplasms / Q000235:genetics* / Q000473:pathology
C495371:TLR9 protein, human; D051195:Toll-Like Receptor 2; D051196:Toll-Like Receptor 3; D051197:Toll-Like Receptor 4; D051217:Toll-Like Receptor 9
PCR; Toll-like receptor; cervical cancer; gene polymorphism