Electroacupuncture improves cerebral blood flow and attenuates moderate ischemic injury via Angiotensin II its receptors-mediated mechanism in rats.
BACKGROUND
To investigate the effects and potential mechanism of electroacupuncture intervention on expressions of Angiotensin II and its receptors-mediated signaling pathway in experimentally induced cerebral ischemia.


METHODS
Totally 126 male Wistar rats were randomly divided into control group, model group and EA group. The latter two were further divided into ten subgroups (n = 6) following Middle Cerebral Artery Occlusion (MCAO). Changes in regional cerebral blood flow (rCBF) and expressions of Angiotensin II and its receptors (AT1R, AT2R), as well as effector proteins in phosphatidyl inositol signal pathway were monitored before and at different times after MCAO.


RESULTS
MCAO-induced decline of ipsilateral rCBF was partially suppressed by electroacupuncture, and contralateral blood flow was also superior to that of model group. Angiotensin II level was remarkably elevated immediately after MCAO, while electroacupuncture group exhibited significantly lower levels at 1 to 3 h and the value was significantly increased thereafter. The enhanced expression of AT1R was partially inhibited by electroacupuncture, while increased AT2R level was further induced. Electroacupuncture stimulation attenuated and postponed the upregulated-expressions of Gq and CaM these upregulations. ELISA results showed sharply increased expressions of DAG and IP3, which were remarkably neutralized by electroacupuncture.


CONCLUSIONS
MCAO induced significant increases in expression of Angiotensin II and its receptor-mediated signal pathway. These enhanced expressions were significantly attenuated by electroacupuncture intervention, followed by reduced vasoconstriction and improved blood supply in ischemic region, and ultimately conferred beneficial effects on cerebral ischemia.
Jing
J
Li
Jiaojun
J
He
Department of Acupuncture and Moxibustion Research Institute, First Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China. jpjs_cn@sina.com.
Yuanhao
Y
Du
Jingjun
J
Cui
Ying
Y
Ma
Xuezhu
X
Zhang
D000804:Angiotensin II / Q000378:metabolism*; D000818:Animals; D002545:Brain Ischemia / Q000378:metabolism / Q000628:therapy*; D002544:Cerebral Infarction / Q000517:prevention & control; D054022:Cerebrum / Q000378:metabolism / Q000473:pathology*; D004195:Disease Models, Animal; D015671:Electroacupuncture; D020244:Infarction, Middle Cerebral Artery; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D011945:Receptors, Angiotensin / Q000378:metabolism*; D012039:Regional Blood Flow; D015854:Up-Regulation
D011945:Receptors, Angiotensin; D000804:Angiotensin II