Acaricidal activity of Ocimum basilicum and Spilanthes acmella against the ectoparasitic tick, Rhipicephalus (Boophilus) microplus (Arachinida: Ixodidae).
The ectoparasitic tick, Rhipicephalus (Boophilus) microplus collected at various cattle farms in and around Chennai was subjected to treatment of different crude solvent extracts of leaves of Ocimum basilicum and Spilanthes acmella for acaricidal activity. Among various solvent extracts of leaves of O. basilicum and S. acmella used, chloroform extract of O. basilicum at concentrations between 6% and 10% exhibited 70% and 100% mortality of ticks when compared to control. The LC50 and LC90 values of the chloroform extract of leaves of O. basilicum treatment on the ticks after 24 h were observed as 5.46% and 7.69%. Quantitative and qualitative analysis of α- and β- carboxylesterase enzymes in the whole gut homogenate of cattle tick, R. microplus treated with chloroform extract of leaves of O. basilicum revealed higher level of activities for the enzymes. This indicated that there was an induced response in the tick, R. microplus against the toxic effects of the extract of O. basilicum.
Department of Zoology, University of Madras, Life Sciences Building, Guindy Campus, Chennai 600025, Tamil Nadu, India.
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Veeramani
Department of Zoology, University of Madras, Life Sciences Building, Guindy Campus, Chennai 600025, Tamil Nadu, India.
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Sakthivelkumar
Department of Zoology, University of Madras, Life Sciences Building, Guindy Campus, Chennai 600025, Tamil Nadu, India.
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Tamilarasan
Department of Zoology, University of Madras, Life Sciences Building, Guindy Campus, Chennai 600025, Tamil Nadu, India.
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Aisha
Department of Zoology, University of Madras, Life Sciences Building, Guindy Campus, Chennai 600025, Tamil Nadu, India.
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Janarthanan
D056810:Acaricides / Q000302:isolation & purification / Q000494:pharmacology*; D000818:Animals; D019659:Asteraceae / Q000737:chemistry*; D001681:Biological Assay; D043182:Carboxylesterase / Q000032:analysis; D041981:Gastrointestinal Tract / Q000201:enzymology; D007194:India; D018649:Ocimum basilicum / Q000737:chemistry*; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry; D048494:Rhipicephalus / Q000187:drug effects* / Q000201:enzymology; D016019:Survival Analysis
D056810:Acaricides; D010936:Plant Extracts; D043182:Carboxylesterase