The efficacy of hydro alcoholic extract of Seidlitzia rosmarinus on experimental zoonotic cutaneous leishmaniasis lesions in murine model.
OBJECTIVE
Leishmaniasis is one of the most important parasitic infectious diseases in the world. Since last century, many efforts have been made to control and treat the disease, but appropriate vaccines, pesticides and medicines are not available or even eligible. The purpose of this study was to evaluate the effect of hydro-alcoholic extract of Seidlitzia rosmarinus on the lesions of experimental Cutaneous Leishmaniasis (CL) in Balb/c mice.


METHODS
The population study was 60 Ballb/c mice which divided to 6 groups, all infected with Leishmania major [MRHO/75/IR]. Soon after the ulcer started to appear in the early stage, a dose of provided herbal extract with 5, 10 and 15% concentration applied on each lesion. The surface area of the lesions measured during an interval of 10 days. Direct Giemsa stained smears prepared two and four weeks after treatment.


RESULTS
Increasing the mean size of the lesions was statistically significant compared to those in control group (p>0.001). Visceral Leishmaniasis (VL) developed in all of the mice including the control group that received Eucerine alone. Survival rate in group receiving 15% S. rosmarinus extracts showed significantly higher compared to mice in control group (p<0.001).


CONCLUSIONS
Hydro-alcoholic extracts of S.rosmarinus with concentrations below15% did not show a therapeutic effect on experimental CL ulcers of Balb/c mice. Further studies with higher concentrations or nano particles are recommended.
Department of Parasitology and Mycology, Emam Reza Hospital, School of Medicine, Mashhad University of Medical Sciences. Mashhad, I. R. Iran.
Maryam
M
Ahmadi
Department of Parasitology and Mycology, Emam Reza Hospital, School of Medicine, Mashhad University of Medical Sciences. Mashhad, I. R. Iran ; Research Center for Skin Diseases & Cutaneous Leishmaniasis, School of Medicine, Mashhad University of Medical Sciences. Mashhad, I. R. Iran.
Abdolmajid
A
Fata
Centre for Research and Training in Skin Diseases and Leprosy, Tehran University of Medical Sciences, Tehran, I. R. Iran.
Ali
A
Khamesipour
Department of Pharmacology and Medicinal plant Research Center, School of Medicine, Mashhad University of Medical Science, Mashhad, I. R. Iran.
Hasan
H
Rakhshandeh
Centre for Research and Training in Skin Diseases and Leprosy, Tehran University of Medical Sciences, Tehran, I. R. Iran.
Akram
A
Miramin Mohammadi
Research Center for Skin Diseases & Cutaneous Leishmaniasis, School of Medicine, Mashhad University of Medical Sciences. Mashhad, I. R. Iran.
Ghodratollah
G
Salehi
Department of Pharmacology and Medicinal plant Research Center, School of Medicine, Mashhad University of Medical Science, Mashhad, I. R. Iran.
Hadi
H
Monavari
Cutaneous Leishmaniasis; Hydro-alcoholic extract; Leishmania major; Seidlitziarosmarinus