[Study on the reference range of reticulocyte multi-parameter in newborn from Beijing].
OBJECTIVE
To explore the RET reference values of newborns in Beijing Area and the relationship between the RET and the day ages of newborns.


METHODS
2 759 (1 404 males and 1 355 females) healthy newborns (born in Beijing Tongren Hospital from January 2007 to December 2012 with 4.31 average day age) were selected, the RET percentage was counted with XE-2100 (adjusted before in use), after the inverse sine transformation of variable's square root, Statistical results show as X⁻ ± s, the reference value refers to bilateral X⁻ ± 1.96 s. The sample was divided into nine groups according to different day ages. Independent sample t served to test and compare the RET difference between two genders newborns, single-factor analysis of variance served to compare the RET difference of the different day ages newborns'groups, Bonferroni adjustment method served to apply the pairwise comparisons. SPSS 18 was used for statistical analysis between males and females across nine age groups.


RESULTS
The normal RET reference values of male and female newborns separately were 2.408%-3.438%, 2.740%-3.912%. RET of male newborns was lower than that of female newborns, the difference was statistically significant (t = -2.564, P < 0.05). The RET difference of different day ages of male and female newborns was statistically significant (F = 138.105, P < 0.01). The comparison of the same day newborns proves that there is RET difference between the male, female newborns of fifth day groups, respectively (2.656 ± 0.067)%, (3.295 ± 0.149)%, there is statistically significant (t = -2.219, P < 0.05) . Newborns' RET vary with day ages, and there is obvious peak value.


CONCLUSIONS
There is RET difference between the male and female newborns, which vary with day ages. The study on the RET reference values of newborns of different genders or different day ages contributes to the clinical disease diagnosis and treatment as reference.
Department of Clinical Laboratory, Beijing Tongren Hospital Affiliated to Capital Medical University, Beijing 100760, China.
Rui
R
Chen
Department of Clinical Laboratory, Beijing Tongren Hospital Affiliated to Capital Medical University, Beijing 100760, China.
Haibin
H
Li
Department of Clinical Laboratory, Beijing Tongren Hospital Affiliated to Capital Medical University, Beijing 100760, China.
Xin
X
Zhang
Department of Clinical Laboratory, Beijing Tongren Hospital Affiliated to Capital Medical University, Beijing 100760, China.
Ling
L
Zhang
Department of Clinical Laboratory, Beijing Tongren Hospital Affiliated to Capital Medical University, Beijing 100760, China.
Zhihai
Z
Wang
Department of Clinical Laboratory, Beijing Tongren Hospital Affiliated to Capital Medical University, Beijing 100760, China. Email: luxinxin2009@126.com.
Xinxin
X
Lu
D005260:Female; D006761:Hospitals; D006801:Humans; D007231:Infant, Newborn; D008297:Male; D012016:Reference Values; D012156:Reticulocytes; D012727:Sex Characteristics