Clinical Presentation and Microarray Analysis of Peruvian Children with Atypical Development and/or Aberrant Behavior.
We report our experience with high resolution microarray analysis in infants and young children with developmental disability and/or aberrant behavior enrolled at the Centro Ann Sullivan del Peru in Lima, Peru, a low income country. Buccal cells were collected with cotton swabs from 233 participants for later DNA isolation and identification of copy number variation (deletions/duplications) and regions of homozygosity (ROH) for estimating consanguinity status in 15 infants and young children (12 males, 3 females; mean age ± SD = 28.1 m ±  7.9 m; age range 14 m-41 m) randomly selected for microarray analysis. An adequate DNA yield was found in about one-half of the enrolled participants. Ten participants showed deletions or duplications containing candidate genes reported to impact behavior or cognitive development. Five children had ROHs which could have harbored recessive gene alleles contributing to their clinical presentation. The coefficient of inbreeding was calculated and three participants showed first-second cousin relationships, indicating consanguinity. Our preliminary study showed that DNA isolated from buccal cells using cotton swabs was suboptimal, but yet in a subset of participants the yield was adequate for high resolution microarray analysis and several genes were found that impact development and behavior and ROHs identified to determine consanguinity status.
Department of Psychiatry and Behavioral Sciences, The University of Kansas Medical Center, 3901 Rainbow Boulevard, MS 4015, Kansas City, KS 66160, USA ; Kansas University Center on Development Disabilities, Schiefelbusch Institute for Life Span Institute, The University of Kansas, Lawrence, KS 66045, USA.
Merlin G
MG
Butler
Department of Psychiatry and Behavioral Sciences, The University of Kansas Medical Center, 3901 Rainbow Boulevard, MS 4015, Kansas City, KS 66160, USA.
Kelly
K
Usrey
Department of Psychiatry and Behavioral Sciences, The University of Kansas Medical Center, 3901 Rainbow Boulevard, MS 4015, Kansas City, KS 66160, USA.
Jennifer L
JL
Roberts
Department of Pediatrics, The University of Kansas Medical Center, Kansas City, KS 66160, USA.
Stephen R
SR
Schroeder
Department of Psychiatry and Behavioral Sciences, The University of Kansas Medical Center, 3901 Rainbow Boulevard, MS 4015, Kansas City, KS 66160, USA.
Ann M
0000-0001-9283-7809
AM
Manzardo