Dynamic fluorescence lifetime imaging based on acousto-optic deflectors.
We report a dynamic fluorescence lifetime imaging (D-FLIM) system that is based on a pair of acousto-optic deflectors for the random regions of interest (ROI) study in the sample. The two-dimensional acousto-optic deflector devices are used to rapidly scan the femtosecond excitation laser beam across the sample, providing specific random access to the ROI. Our experimental results using standard fluorescent dyes in live cancer cells demonstrate that the D-FLIM system can dynamically monitor the changing process of the microenvironment in the ROI in live biological samples.
Wei
W
Yan
Xiao
X
Peng
Jing
J
Qi
Jian
J
Gao
Shunping
S
Fan
Qi
Q
Wang
Junle
J
Qu
Hanben
H
Niu
D004867:Equipment Design; D056945:Hep G2 Cells; D006801:Humans; D008856:Microscopy, Fluorescence / Q000295:instrumentation* / Q000379:methods; D061088:Photoacoustic Techniques / Q000295:instrumentation* / Q000379:methods; D012235:Rhodamines / Q000737:chemistry
D012235:Rhodamines; C026188:rhodamine 6G