Folic acid supplemented goat milk has beneficial effects on hepatic physiology, haematological status and antioxidant defence during chronic Fe repletion.
The aim of the current study was to asses the effect of goat or cow milk-based diets, either normal or Fe-overloaded and folic acid supplement on some aspects of hepatic physiology, enzymatic antioxidant defence and lipid peroxidation in liver, brain and erythrocyte of control and anaemic rats after chronic Fe repletion. 160 male Wistar rats were placed on 40 d in two groups, a control group receiving normal-Fe diet and the Fe-deficient group receiving low Fe diet. Lately, the rats were fed with goat and cow milk-based diets during 30 d, with normal-Fe content or Fe-overload and either with normal folic or folic acid supplemented. Fe-overload increased plasma alanine transaminase and aspartate transaminase levels when cow milk was supplied. Dietary folate supplementation reduced plasma transaminases levels in animals fed goat milk with chronic Fe overload. A remarkable increase in the superoxide dismutase activity was observed in the animals fed cow milk. Dietary folate supplement lead to a decrease on the activity of this enzyme in all the tissues studied with both milk-based diets. A concomitant increment in catalase was also observed. The increase in lipid peroxidation products levels in rats fed cow milk with Fe-overload, suggest an imbalance in the functioning of the enzymatic antioxidant defence. In conclusion, dietary folate-supplemented goat milk reduces both plasma transaminases levels, suggesting a hepatoprotective effect and has beneficial effects in situation of Fe-overload, improving the antioxidant enzymes activities and reducing lipid peroxidation.
Department of Physiology,Faculty of Pharmacy,University of Granada and Institute of Nutrition and Food Technology,Granada 18071,Spain.
María J M
MJ
Alférez
Department of Physiology,Faculty of Pharmacy,University of Granada and Institute of Nutrition and Food Technology,Granada 18071,Spain.
Emilio
E
Rivas
Department of Physiology,Faculty of Pharmacy,University of Granada and Institute of Nutrition and Food Technology,Granada 18071,Spain.
Javier
J
Díaz-Castro
Department of Physiology,Faculty of Pharmacy,University of Granada and Institute of Nutrition and Food Technology,Granada 18071,Spain.
Silvia
S
Hijano
Department of Physiology,Faculty of Pharmacy,University of Granada and Institute of Nutrition and Food Technology,Granada 18071,Spain.
Teresa
T
Nestares
Department of Physiology,Faculty of Pharmacy,University of Granada and Institute of Nutrition and Food Technology,Granada 18071,Spain.
Miguel
M
Moreno
Department of Physiology,Faculty of Pharmacy,University of Granada and Institute of Nutrition and Food Technology,Granada 18071,Spain.
Margarita S
MS
Campos
Department of Physiology,Faculty of Pharmacy,University of Granada and Institute of Nutrition and Food Technology,Granada 18071,Spain.
Jose A
JA
Serrano-Reina
Department of Physiology,Faculty of Pharmacy,University of Granada and Institute of Nutrition and Food Technology,Granada 18071,Spain.
Inmaculada
I
López-Aliaga
D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D001219:Aspartate Aminotransferases / Q000097:blood; D002417:Cattle; D004032:Diet; D019587:Dietary Supplements; D005492:Folic Acid / Q000008:administration & dosage*; D006041:Goats; D006454:Hemoglobins / Q000032:analysis; D000090463:Iron Deficiencies; D019190:Iron Overload; D019266:Iron, Dietary / Q000008:administration & dosage*; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000187:drug effects* / Q000502:physiology; D008297:Male; D008892:Milk / Q000737:chemistry*; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000378:metabolism
D000975:Antioxidants; D006454:Hemoglobins; D019266:Iron, Dietary; D005492:Folic Acid; D013482:Superoxide Dismutase; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase
Goat and cow milk; antioxidant enzymes; folic acid supplementation; lipid peroxidation: chronic iron repletion