Prevalence of human enteroviruses among apparently healthy nursery school children in Accra.
BACKGROUND
Human enteroviruses are common in children causing asymptomatic infections ranging from mild to severe illnesses. In Ghana, information on the prevalence of non-polio enterovirus causing acute flaccid paralysis is available but data on surveillance of these viruses in school children is scanty. Here, the prevalence of human enteroviruses among apparently healthy children in selected school in Accra was studied.


METHODS
Stool samples from 273 apparently healthy children less than eight years of age in 9 selected nursery schools were collected between December 2010 and March 2011 and processed for human enteroviruses on L20B, RD and Hep-2 cell lines. Positive Isolates were characterized by microneutralisation assay with antisera pools from RIVM, the Netherlands according to standard methods recommended by WHO.


RESULTS
Of the 273 samples processed, 66 (24.2%) non-polio enteroviruses were isolated. More growth was seen on Hep-2C (46%) only than RD (18%) only and on both cell lines (34%). No growth was seen on L20B even after blind passage. Excretion of non-polio enteroviruses was found in all the schools with majority in BD school. Serotyping of the isolates yielded predominantly Coxsackie B viruses followed by echoviruses 13 and 7. More than half of the isolates could not be typed by the antisera pools.


CONCLUSIONS
The study detected 13 different serotypes of non-polio enteroviruses in circulation but no poliovirus was found. BD school was found to have the highest prevalence of NPEV. Complete identification through molecular methods is essential to establish the full range of NPEVs in circulation in these schools.
Department Of Microbiology, University of Ghana Medical School, Legon, Ghana ; Ghana Health Service, Ministry of Health, Ghana.
Juliana
J
Attoh
Department of Virology, Noguchi Memorial Institute for Medical Research, University of Ghana, Legon, Ghana.
Evangeline
E
Obodai
Department Of Microbiology, University of Ghana Medical School, Legon, Ghana.
Theophilus
T
Adiku
Department of Virology, Noguchi Memorial Institute for Medical Research, University of Ghana, Legon, Ghana.
John Kofi
JK
Odoom
D000914:Antibodies, Viral / Q000276:immunology; D058070:Asymptomatic Diseases; D002460:Cell Line; D002648:Child; D002675:Child, Preschool; D003384:Coxsackievirus Infections / Q000453:epidemiology / Q000821:virology; D003588:Cytopathogenic Effect, Viral; D003906:Developing Countries; D004770:Enterovirus / Q000145:classification / Q000276:immunology / Q000302:isolation & purification*; D029822:Enterovirus B, Human / Q000276:immunology / Q000302:isolation & purification; D004769:Enterovirus Infections / Q000453:epidemiology* / Q000821:virology; D005243:Feces / Q000821:virology*; D005260:Female; D005869:Ghana / Q000453:epidemiology; D006801:Humans; D007223:Infant; D008297:Male; D011055:Poliovirus Vaccine, Oral; D011204:Poverty Areas; D015995:Prevalence; D012494:Sampling Studies; D012703:Serotyping; D014611:Vaccination; D014776:Virus Cultivation
D000914:Antibodies, Viral; D011055:Poliovirus Vaccine, Oral
Accra; Non-polio enterovirus; apparently healthy; school children