Toxic detection in mine water based on proteomic analysis of lysosomal enzymes in Saccharomyces cerevisiae.
OBJECTIVE
Lysosome is the cell-organelle which is commonly used as biomonitoring tool in environmental pollution. In this study, the lysosomal proteomic of the yeast Saccharomyces cerevisiae was analyzed for utilization in the detection of toxic substances in mine water samples.


METHODS
This work informs the expression of lysosomal proteomic in yeast in response with toxic chemicals, such as sodium meta-arsenite and tetracycline, for screening specific biomarkers. After that, a recombinant yeast contained this biomarker were constructed for toxic detection in pure toxic chemicals and mine water samples.


RESULTS
Each chemical had an optimal dose at which the fluorescent protein intensity reached the peak. In the case of water samples, the yeast showed the response with sample 1, 3, 4, and 5; whereas there is no response with sample 2, 6, and 7.


CONCLUSIONS
The recombinant yeast showed a high ability of toxic detection in response with several chemicals such as heavy metals and pharmaceuticals. In the case of mine water samples, the response varied depending on the sample content.
Department of Bioprocess Engineering, Chonbuk National University, Jeonju, Korea.
Ngoc-Tu
NT
Nguyen
Department of Microbiology, Chungbuk National University, Cheongju, Korea.
Yang-Hoon
YH
Kim
Department of Bioprocess Engineering, Chonbuk National University, Jeonju, Korea.
Seung Hyuck
SH
Bang
Mine Reclamation Corporation, Coal Center, Seoul, Korea.
Ji Hye
JH
Hong
Mine Reclamation Corporation, Coal Center, Seoul, Korea.
Soon Dong
SD
Kwon
Department of Bioprocess Engineering, Chonbuk National University, Jeonju, Korea.
Jiho
J
Min
Heavy metals; Lysosomal proteomic; Lysosomal response; Mine water; Pharmaceuticals