High Resolution Phase-Sensitive Magnetomotive Optical Coherence Microscopy for Tracking Magnetic Microbeads and Cellular Mechanics.
We present a real-time multimodal near-infrared imaging technology that tracks externally induced axial motion of magnetic microbeads in single cells in culture. The integrated multimodal imaging technique consists of phase-sensitive magnetomotive optical coherence microscopy (MM-OCM) and multiphoton microscopy (MPM).MPMis utilized for the visualization of multifunctional fluorescent and magnetic microbeads, while MM-OCM detects, with nanometer-scale sensitivity, periodic displacements of the microbeads induced by the modulation of an external magnetic field. Magnetomotive signals are measured from mouse macrophages, human breast primary ductal carcinoma cells, and human breast epithelial cells in culture, and validated with full-field phase-sensitive microscopy. This methodology demonstrates the capability for imaging controlled cell dynamics and has the potential for measuring cell biomechanical properties, which are important in assessing the health and pathological state of cells.
Department of Physics, University of Illinois at Urbana-Champaign, Urbana, IL 61801 USA ( crecea@illinois.edu ).
Vasilica
V
Crecea
University of Illinois at Urbana-Champaign, Urbana, IL 61801 USA. He is now with NinePoint Medical, Cambridge, MA 02139 USA ( bgraf@ninepointmedical.com ).
Benedikt W
BW
Graf
Department of Electrical and Computer Engineering, University of Illinois at Urbana-Champaign, Urbana, IL 61801 USA ( tkim44@illinois.edu ).
Taewoo
T
Kim
Departments of Electrical and Computer Engineering and Bioengineering, University of Illinois at Urbana-Champaign, Urbana, IL 61801 USA ( gpopescu@illinois.edu ).
Gabriel
G
Popescu
Departments of Electrical and Computer Engineering, Bioengineering, and Internal Medicine, University of Illinois at Urbana-Champaign, Urbana, IL 61801 USA ( boppart@illinois.edu ).
Stephen A
SA
Boppart
Cellular biomechanics; magnetic tweezers; multiphoton microscopy (MPM); optical coherence tomography (OCT); timodal microscopy