Chemotactic signal transduction and phosphate metabolism as adaptive strategies during citrus canker induction by Xanthomonas citri.
The genome of Xanthomonas citri subsp. Citri strain 306 pathotype A (Xac) was completely sequenced more than 10 years; to date, few studies involving functional genomics Xac and its host compatible have been developed, specially related to adaptive events that allow the survival of Xac within the plant. Proteomic analysis of Xac showed that the processes of chemotactic signal transduction and phosphate metabolism are key adaptive strategies during the interaction of a pathogenic bacterium with its plant host. The results also indicate the importance of a group of proteins that may not be directly related to the classical virulence factors, but that are likely fundamental to the success of the initial stages of the infection, such as methyl-accepting chemotaxis protein (Mcp) and phosphate specific transport (Pst). Furthermore, the analysis of the mutant of the gene pstB which codifies to an ABC phosphate transporter subunit revealed a complete absence of citrus canker symptoms when inoculated in compatible hosts. We also conducted an in silico analysis which established the possible network of genes regulated by two-component systems PhoPQ and PhoBR (related to phosphate metabolism), and possible transcriptional factor binding site (TFBS) motifs of regulatory proteins PhoB and PhoP, detaching high degree of conservation of PhoB TFBS in 84 genes of Xac genome. This is the first time that chemotaxis signal transduction and phosphate metabolism were therefore indicated to be fundamental to the process of colonization of plant tissue during the induction of disease associated with Xanthomonas genus bacteria.
Departamento de Ciências Biológicas, Instituto de Ciências Exatas e Biológicas, Universidade Federal de Ouro Preto, Ouro Preto, MG, Brazil.
Leandro Marcio
LM
Moreira
Agda Paula
AP
Facincani
Cristiano Barbalho
CB
Ferreira
Rafael Marine
RM
Ferreira
Maria Inês Tiraboshi
MI
Ferro
Fabio Cesar
FC
Gozzo
Julio Cezar Franco
JC
de Oliveira
Jesus Aparecido
JA
Ferro
Márcia Regina
MR
Soares
D000220:Adaptation, Biological; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D001665:Binding Sites; D002633:Chemotaxis; D002957:Citrus / Q000382:microbiology*; D005407:Flagella / Q000502:physiology; D009154:Mutation; D010710:Phosphates / Q000378:metabolism*; D010935:Plant Diseases / Q000382:microbiology*; D018085:Regulon; D015398:Signal Transduction; D014157:Transcription Factors / Q000378:metabolism; D014974:Xanthomonas / Q000235:genetics / Q000378:metabolism* / Q000472:pathogenicity / Q000502:physiology
D001426:Bacterial Proteins; D010710:Phosphates; D014157:Transcription Factors; C061278:PhoB protein, Bacteria; C061064:PhoP protein, Bacteria