Inter-SINE Amplified Polymorphism (ISAP) for rapid and robust plant genotyping.
The unambiguous differentiation of crop genotypes is often laborious or expensive. A rapid, robust, and cost-efficient marker system is required for routine genotyping in plant breeding and marker-assisted selection. We describe the Inter-SINE Amplified Polymorphism (ISAP) system that is based on standard molecular methods resulting in genotype-specific fingerprints at high resolution. These markers are derived from Short Interspersed Nuclear Elements (SINEs) which are dispersed repetitive sequences present in most if not all plant genomes and can be efficiently extracted from plant genome sequences. The ISAP method was developed on potato as model plant but is also transferable to other plant species.
Institute of Botany, Technische Universität Dresden, Mommsenstraße 11, Dresden, 01062, Germany.
Torsten
T
Wenke
Kathrin M
KM
Seibt
Thomas
T
Döbel
Katja
K
Muders
Thomas
T
Schmidt
D001483:Base Sequence; D017931:DNA Primers / Q000378:metabolism; D004587:Electrophoresis, Agar Gel; D019075:Electrophoresis, Capillary; D060005:Genotyping Techniques / Q000379:methods*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D016415:Sequence Alignment; D020086:Short Interspersed Nucleotide Elements / Q000235:genetics*; D011198:Solanum tuberosum / Q000235:genetics*; D013223:Statistics as Topic
D017931:DNA Primers