The effect of Astragalus extractive on alveolar bone rebuilding progress of tooth extracted socket of ovariectomied rats.
BACKGROUND
Postmenopausal osteoporosis (PMO) is an estrogen deficiency condition that causes severe loss of bone mass in the vertebrae and long bones. We explored the effect and the possible underlying mechanism of the extracts of Astragalus (AE) on the tooth alveolar bone rebuilding progress of postmenopausal osteoporosis of PMO animal models.


METHODS
The PMO models were acquired by ovariectomy. After 8 weeks the first left maxillary molars of the rats were extracted and AE was administered orally for 8 weeks. Then the histological morphology and the transcription and expression of TGF-β1 and TNF-α in the tooth extracted socket were detected by HE dying, QRT-PCR and ELISA.


RESULTS
The results showed that the new bone volume and TGF-β1 was significantly lower in PMO group than the control group and AE group at the observing period. At the same time TNF-α in PMO group was significantly higher than the other two groups. Moreover AE group had no significant differences with the control group in all data at the observing period.


CONCLUSIONS
AE has positive effect on alveolar bone rebuilding progress of tooth extracted socket of PMO rats. AE also has the potential to enhance the expression of TGF-β1 and decrease the expression of TNF-α of the rebuilding tooth extracted socket.
Stomatology Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang, China.
Xiaochen
X
Liu
Women's Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang, China.
Runju
R
Zhang
Stomatology Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang, China.
Yi
Y
Zhou
Stomatology Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang, China.
Yanli
Y
Yang
Stomatology Hospital?Xian Jiaotong University, Xian, Shaanxi, China.
Hongling
H
Si
Stomatology Hospital?Xian Jiaotong University, Xian, Shaanxi, China.
Xiaohong
X
Li
Stomatology Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang, China.
Li
L
Liu
D000818:Animals; D027883:Astragalus Plant; D004195:Disease Models, Animal; D005260:Female; D006801:Humans; D010012:Osteogenesis / Q000187:drug effects*; D015663:Osteoporosis, Postmenopausal / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D010052:Ovariectomy; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D014081:Tooth Extraction; D020390:Tooth Socket / Q000187:drug effects* / Q000473:pathology; D014158:Transcription, Genetic; D053773:Transforming Growth Factor beta1 / Q000378:metabolism*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
D010936:Plant Extracts; D053773:Transforming Growth Factor beta1; D014409:Tumor Necrosis Factor-alpha
Astragalus; PMO; TGF-β1; TNF-α