Graded behavioral responses and habituation to sound in the common cuttlefish Sepia officinalis.
Sound is a widely available and vital cue in aquatic environments, yet most bioacoustic research has focused on marine vertebrates, leaving sound detection in invertebrates poorly understood. Cephalopods are an ecologically key taxon that likely use sound and may be impacted by increasing anthropogenic ocean noise, but little is known regarding their behavioral responses or adaptations to sound stimuli. These experiments identify the acoustic range and levels that elicit a wide range of secondary defense behaviors such as inking, jetting and rapid coloration change. Secondarily, it was found that cuttlefish habituate to certain sound stimuli. The present study examined the behavioral responses of 22 cuttlefish (Sepia officinalis) to pure-tone pips ranging from 80 to 1000 Hz with sound pressure levels of 85-188 dB re. 1 μPa rms and particle accelerations of 0-17.1 m s(-2). Cuttlefish escape responses (inking, jetting) were observed between frequencies of 80 and 300 Hz and at sound levels above 140 dB re. 1 μPa rms and 0.01 m s(-2) (0.74 m s(-2) for inking responses). Body patterning changes and fin movements were observed at all frequencies and sound levels. Response intensity was dependent upon stimulus amplitude and frequency, suggesting that cuttlefish also possess loudness perception with a maximum sensitivity around 150 Hz. Cuttlefish habituated to repeated 200 Hz tone pips, at two sound intensities. Total response inhibition was not reached, however, and a basal response remained present in most animals. The graded responses provide a loudness sensitivity curve and suggest an ecological function for sound use in cephalopods.
Biology Department, Woods Hole Oceanographic Institution, Woods Hole, MA 02543, USA. Experimental Zoology Group, Wageningen University, De Elst 1, 6708WD Wageningen, The Netherlands.
Julia E
JE
Samson
Biology Department, Woods Hole Oceanographic Institution, Woods Hole, MA 02543, USA. Program in Sensory Physiology and Behavior, Marine Biological Laboratory, Woods Hole, MA, 02543, USA. amooney@whoi.edu.
T Aran
TA
Mooney
Experimental Zoology Group, Wageningen University, De Elst 1, 6708WD Wageningen, The Netherlands.
Sander W S
SW
Gussekloo
Program in Sensory Physiology and Behavior, Marine Biological Laboratory, Woods Hole, MA, 02543, USA.
Roger T
RT
Hanlon
D000161:Acoustic Stimulation; D000222:Adaptation, Physiological / Q000502:physiology*; D000818:Animals; D001522:Behavior, Animal; D004924:Escape Reaction / Q000502:physiology*; D006185:Habituation, Psychophysiologic; D006309:Hearing / Q000502:physiology; D049833:Sepia / Q000502:physiology*; D013016:Sound / Q000009:adverse effects
Bioacoustics; Cephalopod; Ear; Hearing; Invertebrate; Lateral line; Loudness; Noise; Statocyst