Follicular steroid hormones as markers of oocyte quality and oocyte development potential.
BACKGROUND
Various components of follicular fluid are suggested as biochemical predictors of oocyte quality. Previous studies of follicular steroid hormone levels have shown disparate results when related with fertilization outcomes.


OBJECTIVE
The objective of the study was to relate the levels of steroid hormones of each individual follicle with oocyte maturation, fertilization results, embryo quality, and pregnancy rates.


METHODS
Prospective cohort study in a university hospital.


METHODS
In 31 patients, who underwent intracytoplasmic sperm injection, it was performed an ultrasound guided aspiration of follicular fluid of the first two mature follicles from each ovary. Follicular levels of estradiol, progesterone, testosterone, and dehydroepiandrosterone sulfate were measured by chemiluminescent immunoassay.


METHODS
Generalized estimating equation model.


RESULTS
In follicular fluids with mature oocyte presence, in normal as well as in failed fertilization, there was a positive correlation between follicular testosterone and progesterone (r = 0.794, P = 0.0001 and r = 0.829, P = 0.0001). Progesterone levels were higher in cases of normal fertilization compared to failed fertilization (P = 0.003). B quality embryos came from oocytes immersed in follicular fluids with higher estradiol values and higher estradiol/progesterone and estradiol/testosterone ratios than those of C quality (P = 0.01; P = 0.0009; P = 0.001). Estradiol levels were higher in patients who achieved pregnancy (P = 0.02).


CONCLUSIONS
The analysis of follicular hormone composition could be considered as an additional tool in oocyte selection.
Department of Obstetrics, Gynaecology and Reproduction, La Paz University Hospital, Paseo de la Castellana, Madrid, Spain.
Nayara López
NL
Carpintero
Department of Obstetrics, Gynaecology and Reproduction, La Paz University Hospital, Paseo de la Castellana, Madrid, Spain.
Onica Armijo
OA
Suárez
Department of Obstetrics, Gynaecology and Reproduction, La Paz University Hospital, Paseo de la Castellana, Madrid, Spain.
Carmen Cuadrado
CC
Mangas
Department of Obstetrics, Gynaecology and Reproduction, La Paz University Hospital, Paseo de la Castellana, Madrid, Spain.
Carolina González
CG
Varea
Department of Clinical Analyzes, La Paz University Hospital, Paseo de la Castellana, Madrid, Spain.
Rubén Gómez
RG
Rioja
Fertilisation; follicular fluid; oocyte maturation; pregnancy; steroid