Screening of differential promoter hypermethylated genes in primary oral squamous cell carcinoma.
BACKGROUND
Promoter hypermethylation leads to altered gene functions and may result in malignant cellular transformation. Thus, identification of biomarkers for hypermethylated genes could be useful for diagnosis, prognosis, and therapeutic treatment of oral squamous cell carcinoma (OSCC).


OBJECTIVE
To screen hypermethylated genes with a microarray approach and to validate selected hypermethylated genes with the methylation-specific polymerase chain reaction (MSPCR).


METHODS
Genome-wide analysis of normal oral mucosa and OSCC tissues was conducted using the Illumina methylation microarray. The specified differential genes were selected and hypermethylation status was further verified with an independent cohort sample of OSCC samples. Candidate genes were screened using microarray assay and run by MSPCR analysis.


RESULTS
TP73, PIK3R5, and CELSR3 demonstrated high percentages of differential hypermethylation status.


CONCLUSIONS
Our microarray screening and MSPCR approaches revealed that the signature candidates of differentially hypermethylated genes may possibly become potential biomarkers which would be useful for diagnostic, prognostic and therapeutic targets of OSCC in the near future.
Centre of Preclinical Science Studies, Faculty of Medicine, Universiti Teknologi MARA, Selangor, Malaysia E-mail : gootheah@salam.uitm.edu.my; thongkl@.um.edu.my.
Goot Heah
GH
Khor
Gabrielle Ruth Anisah
GR
Froemming
Rosnah Binti
RB
Zain
Mannil Thomas
MT
Abraham
Kwai Lin
KL
Thong
D014408:Biomarkers, Tumor / Q000235:genetics*; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000473:pathology; D016022:Case-Control Studies; D019175:DNA Methylation; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D009061:Mouth Mucosa / Q000378:metabolism; D009062:Mouth Neoplasms / Q000235:genetics* / Q000473:pathology; D020411:Oligonucleotide Array Sequence Analysis; D016133:Polymerase Chain Reaction; D011379:Prognosis; D011401:Promoter Regions, Genetic / Q000235:genetics*
D014408:Biomarkers, Tumor