Characterization of a subpopulation of developing cortical interneurons from human iPSCs within serum-free embryoid bodies.
Production and isolation of forebrain interneuron progenitors are essential for understanding cortical development and developing cell-based therapies for developmental and neurodegenerative disorders. We demonstrate production of a population of putative calretinin-positive bipolar interneurons that express markers consistent with caudal ganglionic eminence identities. Using serum-free embryoid bodies (SFEBs) generated from human inducible pluripotent stem cells (iPSCs), we demonstrate that these interneuron progenitors exhibit morphological, immunocytochemical, and electrophysiological hallmarks of developing cortical interneurons. Finally, we develop a fluorescence-activated cell-sorting strategy to isolate interneuron progenitors from SFEBs to allow development of a purified population of these cells. Identification of this critical neuronal cell type within iPSC-derived SFEBs is an important and novel step in describing cortical development in this iPSC preparation.
New York Stem Cell Foundation Laboratory, New York, New York; The Hussman Institute for Autism, Baltimore, Maryland mnestor@hussmanautism.org.
Michael W
MW
Nestor
New York Stem Cell Foundation Laboratory, New York, New York;
Samson
S
Jacob
New York Stem Cell Foundation Laboratory, New York, New York;
Bruce
B
Sun
Department of Pathology and Cell Biology and Taub Institute for Research on Alzheimer's Disease and the Aging Brain, Columbia University, New York, New York;
Deborah
D
Prè
New York Stem Cell Foundation Laboratory, New York, New York;
Andrew A
AA
Sproul
Department of Biological Science, Hunter College, New York, New York;
Seong Im
SI
Hong
New York Stem Cell Foundation Laboratory, New York, New York;
Chris
C
Woodard
New York Stem Cell Foundation Laboratory, New York, New York;
Matthew
M
Zimmer
Department of Pathology and Cell Biology and Taub Institute for Research on Alzheimer's Disease and the Aging Brain, Columbia University, New York, New York; Research Center for Neuroscience, Institute of Molecular Biosciences, Mahidol University, Salaya, Nakhonpathom, Thailand; and.
Vorapin
V
Chinchalongporn
Department of Pathology and Cell Biology and Taub Institute for Research on Alzheimer's Disease and the Aging Brain, Columbia University, New York, New York;
Ottavio
O
Arancio
New York Stem Cell Foundation Laboratory, New York, New York;
Scott A
SA
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D000818:Animals; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology* / Q000502:physiology*; D058732:Embryoid Bodies / Q000502:physiology*; D005347:Fibroblasts / Q000502:physiology; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000502:physiology*; D007395:Interneurons / Q000502:physiology*; D051379:Mice; D018345:Mice, Knockout
calretinin; cortex; inducible pluripotent stem cell; interneuron; neuron progenitor; serum-free embryoid body