Expression and function of GSTA1 in lung cancer cells.
Glutathione S-transferase A1 (GSTA1) appears to be primarily involved in detoxification processes, but possible roles in lung cancer remain unclear. The objective of this study was to investigate the expression and function of GSTA1 in lung cancer cells. Real-time PCR and Western blotting were performed to assess expression in cancer cell lines and the normal lung cells, then verify the A549 cells line with stable overexpression. Localization of GSTA1 proteins was assessed by cytoimmunofluorescence. Three double-strand DNA oligoRNAs (SiRNAs) were synthesized prior to being transfected into A549 cells with Lipofectamine 2000, and then the most efficient SiRNA was selected. Expression of the GSTA1 gene in the transfected cells was determined by real-time PCR and Western blotting. The viability of the transfected cells were assessed by MTT. Results showed that the mRNA and protein expression of A549 cancer cells was higher than in MRC-5 normal cells. Cytoimmunofluorescence demonstrated GSTA1 localization in the cell cytoplasm and/or membranes. Transfection into A549 cells demonstrated that down-regulated expression could inhibit cell viability. Our data indicated that GSTA1 expression may be a target molecule in early diagnosis and treatment of lung cancer.
College of Pharmacy, Guang dong Pharmaceutical University , Guangzhou, China E-mail : zp657493@163.com.
Xue-Diao
XD
Pan
Zhou-Ping
ZP
Yang
Qi-Ling
QL
Tang
Tong
T
Peng
Zheng-Bing
ZB
Zhang
Si-Gui
SG
Zhou
Gui-Xiang
GX
Wang
Bing
B
He
Lin-Quan
LQ
Zang
D000704:Analysis of Variance; D015153:Blotting, Western; D002470:Cell Survival / Q000235:genetics; D055088:Early Detection of Cancer / Q000379:methods*; D005455:Fluorescent Antibody Technique; D015972:Gene Expression Regulation, Neoplastic; D005819:Genetic Markers / Q000235:genetics*; D005982:Glutathione Transferase / Q000235:genetics*; D006801:Humans; D008175:Lung Neoplasms / Q000175:diagnosis / Q000235:genetics* / Q000473:pathology* / Q000628:therapy; D034741:RNA, Small Interfering / Q000032:analysis / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods; D012680:Sensitivity and Specificity; D014162:Transfection; D014407:Tumor Cells, Cultured; D015854:Up-Regulation
D005819:Genetic Markers; D034741:RNA, Small Interfering; C484555:GSTA1 protein, human; D005982:Glutathione Transferase