Liver growth factor induces testicular regeneration in EDS-treated rats and increases protein levels of class B scavenger receptors.
The aim of the present work was to determine the effects of liver growth factor (LGF) on the regeneration process of rat testes after chemical castration induced by ethane dimethanesulfonate (EDS) by analyzing some of the most relevant proteins involved in cholesterol metabolism, such as hormone sensitive lipase (HSL), 3β-hydroxysteroid dehydrogenase (3β-HSD), scavenger receptor SR-BI, and other components of the SR family that could contribute to the recovery of steroidogenesis and spermatogenesis in the testis. Sixty male rats were randomized to nontreated (controls) and LGF-treated, EDS-treated, and EDS + LGF-treated groups. Testes were obtained on days 10 (T1), 21 (T2), and 35 (T3) after EDS treatment, embedded in paraffin, and analyzed by immunohistochemistry and Western blot. LGF improved the recovery of the seminiferous epithelia, the appearance of the mature pattern of Leydig cell interstitial distribution, and the expression of mature SR-BI. Moreover, LGF treatment resulted in partial recovery of HSL expression in Leydig cells and spermatogonia. No changes in serum testosterone were observed in control or LGF-treated rats, but in EDS-castrated animals LGF treatment induced a progressive increase in serum testosterone levels and 3β-HSD expression. Based on the pivotal role of SR-BI in the uptake of cholesteryl esters from HDL, it is suggested that the observed effects of LGF would facilitate the provision of cholesterol for sperm cell growth and Leydig cell recovery.
Departamento de Biomedicina y Biotecnología, Universidad de Alcalá, Alcalá de Henares, Madrid, Spain;
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MV
Lobo
Departamento de Biomedicina y Biotecnología, Universidad de Alcalá, Alcalá de Henares, Madrid, Spain;
M I
MI
Arenas
Servicio de Bioquímica-Departamento Investigación, Hospital Universitario Ramón y Cajal, Instituto Ramón y Cajal de Investigación Sanitaria, CIBER de Fisiopatología de la Obesidad y Nutrición, Instituto de Salud Carlos III, Madrid, Spain;
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L
Huerta
Servicio de Bioquímica-Departamento Investigación, Hospital Universitario Ramón y Cajal, Instituto Ramón y Cajal de Investigación Sanitaria, CIBER de Fisiopatología de la Obesidad y Nutrición, Instituto de Salud Carlos III, Madrid, Spain;
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Sacristán
Departamento de Reproducción Animal y Conservación de Recursos Zoogenéticos, INIA, Madrid, Spain; and.
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M
Pérez-Crespo
Departamento de Reproducción Animal y Conservación de Recursos Zoogenéticos, INIA, Madrid, Spain; and.
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Gutiérrez-Adán
Servicio de Bioquímica Experimental, Hospital Universitario Puerta de Hierro Majadahonda, Madrid, Spain.
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JJ
Díaz-Gil
Servicio de Bioquímica-Departamento Investigación, Hospital Universitario Ramón y Cajal, Instituto Ramón y Cajal de Investigación Sanitaria, CIBER de Fisiopatología de la Obesidad y Nutrición, Instituto de Salud Carlos III, Madrid, Spain;
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MA
Lasunción
Servicio de Bioquímica-Departamento Investigación, Hospital Universitario Ramón y Cajal, Instituto Ramón y Cajal de Investigación Sanitaria, CIBER de Fisiopatología de la Obesidad y Nutrición, Instituto de Salud Carlos III, Madrid, Spain; antonia.martin@hrc.es.
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Martín-Hidalgo
D000818:Animals; D001663:Bilirubin / Q000494:pharmacology*; D015153:Blotting, Western; D018955:CD36 Antigens / Q000378:metabolism*; D007150:Immunohistochemistry; D007985:Leydig Cells / Q000378:metabolism*; D008297:Male; D008698:Mesylates / Q000008:administration & dosage; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D012709:Serum Albumin / Q000494:pharmacology*; D000075462:Serum Albumin, Human; D013081:Sperm Motility; D013091:Spermatogenesis / Q000502:physiology*; D002787:Sterol Esterase / Q000378:metabolism*; D013737:Testis / Q000166:cytology / Q000378:metabolism*; D013739:Testosterone / Q000097:blood
D018955:CD36 Antigens; D008698:Mesylates; D012709:Serum Albumin; C053498:albumin-bilirubin complex; D013739:Testosterone; D002787:Sterol Esterase; C002994:ethylene dimethanesulfonate; D001663:Bilirubin; D000075462:Serum Albumin, Human
cholesterol esters; class B scavenger receptor; epididymis; ethane 1,2-dimethane sulphonate; fertility; hormone-sensitive lipase; liver growth factor; lysosomal integral membrane protein II; scavenger receptor-BI; scavenger receptor-BII; spermatogenesis; sterility; steroidogenesis; testis