Immunopreventive effects against murine H22 hepatocellular carcinoma in vivo by a DNA vaccine targeting a gastrin- releasing peptide.
There is a continuing need for innovative alternative therapies for liver cancer. DNA vaccines for hormone/ growth factor immune deprivation represent a feasible and attractive approach for cancer treatment. We reported a preventive effect of a DNA vaccine based on six copies of the B cell epitope GRP18-27 with optimized adjuvants against H22 hepatocarcinoma. Vaccination with pCR3.1-VS-HSP65-TP-GRP6-M2 (vaccine) elicited much higher level of anti-GRP antibodies and proved efficacious in preventing growth of transplanted hepatocarcinoma cells. The tumor size and weight were significantly lower (p<0.05) in the vaccine subgroup than in the control pCR3.1-VS-TP-HSP65-TP-GRP6, pCR3.1-VS-TP-HSP65-TP-M2 or saline subgroups. In addition, significant reduction of tumor-induced angiogenesis associated with intradermal tumors of H22 cells was observed. These potent effects may open ways towards the development of new immunotherapeutic approaches in the treatment of liver cancer.
School of Life Science and Technology, China Pharmaceutical University, Nanjing China E-mail : didicpu@gmail.com.
Jean Louis Didier
JL
Meko'o
Yun
Y
Xing
Huiyong
H
Zhang
Yong
Y
Lu
Jie
J
Wu
Rongyue
R
Cao
D000276:Adjuvants, Immunologic; D000818:Animals; D000906:Antibodies / Q000097:blood / Q000276:immunology; D019496:Cancer Vaccines / Q000008:administration & dosage* / Q000276:immunology; D006528:Carcinoma, Hepatocellular / Q000235:genetics / Q000276:immunology / Q000517:prevention & control*; D019886:Gastrin-Releasing Peptide / Q000037:antagonists & inhibitors* / Q000235:genetics; D007114:Immunization; D008113:Liver Neoplasms / Q000235:genetics / Q000276:immunology / Q000517:prevention & control*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009389:Neovascularization, Pathologic / Q000517:prevention & control*; D014407:Tumor Cells, Cultured; D019444:Vaccines, DNA / Q000008:administration & dosage* / Q000276:immunology
D000276:Adjuvants, Immunologic; D000906:Antibodies; D019496:Cancer Vaccines; D019444:Vaccines, DNA; D019886:Gastrin-Releasing Peptide