Response of Daphnia's antioxidant system to spatial heterogeneity in Cyanobacteria concentrations in a lowland reservoir.
Many species and clones of Daphnia inhabit ecosystems with permanent algal blooms, and they can develop tolerance to cyanobacterial toxins. In the current study, we examined the spatial differences in the response of Daphnia longispina to the toxic Microcystis aeruginosa in a lowland eutrophic dam reservoir between June (before blooms) and September (during blooms). The reservoir showed a distinct spatial pattern in cyanobacteria abundance resulting from the wind direction: the station closest to the dam was characterised by persistently high Microcystis biomass, whereas the upstream stations had a significantly lower biomass of Microcystis. Microcystin concentrations were closely correlated with the cyanobacteria abundance (r = 0.93). The density of daphniids did not differ among the stations. The main objective of this study was to investigate how the distribution of toxic Microcystis blooms affects the antioxidant system of Daphnia. We examined catalase (CAT) activity, the level of the low molecular weight antioxidant glutathione (GSH), glutathione S-transferase (GST) activity and oxidative stress parameters, such as lipid peroxidation (LPO). We found that the higher the abundance (and toxicity) of the cyanobacteria, the lower the values of the antioxidant parameters. The CAT activity and LPO level were always significantly lower at the station with the highest M. aeruginosa biomass, which indicated the low oxidative stress of D. longispina at the site with the potentially high toxic thread. However, the low concentration of GSH and the highest activity of GST indicated the occurrence of detoxification processes at this site. These results demonstrate that daphniids that have coexisted with a high biomass of toxic cyanobacteria have effective mechanisms that protect them against the toxic effects of microcystins. We also conclude that Daphnia's resistance capacity to Microcystis toxins may differ within an ecosystem, depending on the bloom's spatial distribution.
Department of Applied Ecology, University of Lodz, Lodz, Poland.
Adrianna
A
Wojtal-Frankiewicz
Department of Molecular Biophysics, University of Lodz, Lodz, Poland.
Joanna
J
Bernasińska
Department of Applied Ecology, University of Lodz, Lodz, Poland.
Piotr
P
Frankiewicz
Department of Molecular Biophysics, University of Lodz, Lodz, Poland.
Krzysztof
K
Gwoździński
Department of Applied Ecology, University of Lodz, Lodz, Poland.
Tomasz
T
Jurczak
D000222:Adaptation, Physiological / Q000187:drug effects; D000704:Analysis of Variance; D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D001427:Bacterial Toxins / Q000378:metabolism / Q000633:toxicity; D018533:Biomass; D002374:Catalase / Q000378:metabolism; D000087522:Cyanobacteria Toxins; D003621:Daphnia / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism*; D017753:Ecosystem; D005068:Eutrophication; D005618:Fresh Water / Q000382:microbiology / Q000469:parasitology; D005843:Geography; D005978:Glutathione / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism; D015227:Lipid Peroxidation; D008387:Marine Toxins / Q000378:metabolism / Q000633:toxicity; D052998:Microcystins / Q000378:metabolism / Q000633:toxicity; D046931:Microcystis / Q000254:growth & development*; D011044:Poland; D011156:Population Density; D012621:Seasons
D000975:Antioxidants; D001427:Bacterial Toxins; D000087522:Cyanobacteria Toxins; D008387:Marine Toxins; D052998:Microcystins; D002374:Catalase; D005982:Glutathione Transferase; D005978:Glutathione