Electromigrative separation techniques in forensic science: combining selectivity, sensitivity, and robustness.
In this review we introduce the advantages and limitations of electromigrative separation techniques in forensic toxicology. We thus present a summary of illustrative studies and our own experience in the field together with established methods from the German Federal Criminal Police Office rather than a complete survey. We focus on the analytical aspects of analytes' physicochemical characteristics (e.g. polarity, stereoisomers) and analytical challenges including matrix tolerance, separation from compounds present in large excess, sample volumes, and orthogonality. For these aspects we want to reveal the specific advantages over more traditional methods. Both detailed studies and profiling and screening studies are taken into account. Care was taken to nearly exclusively document well-validated methods outstanding for the analytical challenge discussed. Special attention was paid to aspects exclusive to electromigrative separation techniques, including the use of the mobility axis, the potential for on-site instrumentation, and the capillary format for immunoassays. The review concludes with an introductory guide to method development for different separation modes, presenting typical buffer systems as starting points for different analyte classes. The objective of this review is to provide an orientation for users in separation science considering using capillary electrophoresis in their laboratory in the future.
Forschungszentrum Jülich GmbH, Central Institute for Engineering, Electronics and Analytics, Analytics ZEA-3, 52425, Jülich, Germany.
Tjorben Nils
TN
Posch
Michael
M
Pütz
Nathalie
N
Martin
Carolin
C
Huhn
D004586:Electrophoresis / Q000379:methods*; D044707:Forensic Sciences / Q000379:methods*; D006801:Humans; D012680:Sensitivity and Specificity