Hair follicle targeting, penetration enhancement and Langerhans cell activation make cyanoacrylate skin surface stripping a promising delivery technique for transcutaneous immunization with large molecules and particle-based vaccines.
Transcutaneous immunization (TCI) requires targeting of a maximum number of skin antigen-presenting cells as non-invasive as possible on small skin areas. In two clinical trials, we introduced cyanoacrylate skin surface stripping (CSSS) as a safe method for TCI. Here, using ex vivo human skin, we demonstrate that one CSSS procedure removed only 30% of stratum corneum, but significantly increased the penetration of 200 nm polystyrene particles deep into vellus and intermediate hair follicles from where they could not been retrieved by conventional tape stripping. Two subsequent CSSS had no striking additional effect. CSSS increased particle penetration in superficial stratum corneum and induced Langerhans cell activation. Formulation in amphiphilic ointment or massage did not substantially influences the interfollicular penetration profiles. Hair follicle (HF) targeting by CSSS could become a highly effective tool for TCI when combined with carrier-based delivery and is gaining new attention as our understanding on the HF immune system increases.
Department of Dermatology and Allergy, Clinical Research Center for Hair and Skin Science, Charité-Universitätsmedizin Berlin, Berlin, Germany; UMR S CR7, Centre d¹Immunologie et de Maladies Infectieuses Paris (Cimi-Paris), UPMC University Paris 06, Sorbonne Universités, Paris, France; INSERM U1135, Cimi-Paris, Paris, France.
Annika
A
Vogt
Sabrina
S
Hadam
Iliane
I
Deckert
Julia
J
Schmidt
Andrea
A
Stroux
Zahra
Z
Afraz
Fiorenza
F
Rancan
Jürgen
J
Lademann
Behazine
B
Combadiere
Ulrike
U
Blume-Peytavi
D000279:Administration, Cutaneous; D018949:Antigens, CD1 / Q000378:metabolism; D002465:Cell Movement; D003487:Cyanoacrylates / Q000737:chemistry*; D004337:Drug Carriers / Q000737:chemistry; D016503:Drug Delivery Systems; D004817:Epidermis / Q000276:immunology / Q000378:metabolism; D018859:Hair Follicle / Q000473:pathology*; D064368:Healthy Volunteers; D006801:Humans; D007114:Immunization; D007801:Langerhans Cells / Q000166:cytology*; D053758:Nanoparticles / Q000737:chemistry; D036103:Nanotechnology; D011137:Polystyrenes / Q000737:chemistry; D012867:Skin / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D014612:Vaccines / Q000737:chemistry
D018949:Antigens, CD1; C419393:CD1a antigen; D003487:Cyanoacrylates; D004337:Drug Carriers; D011137:Polystyrenes; D014612:Vaccines
hair follicle targeting; nanocarriers; nanoparticles; transcutaneous vaccination