Antibiotic resistance in uropathogenic e. Coli strains isolated from non-hospitalized patients in pakistan.
OBJECTIVE
To study multidrug-resistance in Uropathogenic E. Coli (UPEC) isolated from non-hospitalized patients.


METHODS
Altogether, 250 bacterial samples were collected from non-hospitalized patients. Their identifications were done on basis of Gram-staining, colony morphology, biochemical testing and PCR. Susceptibility testing was performed by using standard protocols which were recommended by CLSI.


METHODS
For comparisons, statistical analysis was performed by using software, Graphpad Prism 5.0 RESULTS: In total, 32% (n = 80) of the isolates were identified as E. Coli strains and their susceptibility patterns for different antibiotics were determined. The data indicated least resistance against tazocin [(TZP) -1.25%], amikacin [(AK) -1.8%], tigecycline [(TGC)- 2.5%] and nitrofurantoin [(F) -3.75%]. For both minocycline (MH) and sulzone (SUL), resistance rate was 5%, for gentamicin (CN), it was 16.25%, while higher resistances were observed against cephalothine [(KF)- 70%], cefotaxime [(CTX) -58.5%], ceftazidime [(CAZ)- 57.5%], cefepime [(FEP) -55%], cefuroxime and cefixime [(CXM) (CFM)- 53.75 %]. Resistance against ciprofloxacin (CIP) was 57.5%, for norfloxacine (NOR), it was 52.5% and incase of sparfloxacin (SPX), it remained 55%. High percentage of the isolates were resistant to cotrimoxazole [(SXT) -86%] and Amoxicillin [AMX-CLA (AMC)- 76%]. No resistance against meropenem (MEM) was observed.


CONCLUSIONS
Highest level of drug-resistance was observed against trimethoprim-sulfamethoxazole (TMP-SMZ) among clinical isolates of uropathogenic E. Coli collected from non-hospitalized patients.
PhD Student, Department of Microbiology, Quaid-i-Azam University , Islamabad .
Ihsan
I
Ali
M. Phil Student, Department of Microbiology, Quaid-i-Azam University , Islamabad .
Neeraj
N
Kumar
Professor, Department of Microbiology, Quaid-i-Azam University , Islamabad .
Safia
S
Ahmed
Assistant Professor, Department of Microbiology, Quaid-i-Azam University , Islamabad .
Javid Iqbal
JI
Dasti
Antibiotic susceptibility; Beta-lactamase; Cotrimoxazole; E. Coli; Plasmids; UPEC