A new multienzyme-type biosensor for triglyceride determination.
An amperometric multienzyme biosensor for determination of triglycerides (TGs) was constructed by mounting three gelatin membrane-bound enzymes on a glassy carbon electrode (working electrode), then connecting it to electrometer along with an Ag/AgCl reference electrode and a Pt auxiliary electrode. Characterization and optimization of the multienzyme biosensor, which is prepared with glycerol kinase (GK) (E.C.2.7.1.30), glycerol-3-phosphate oxidase (GPO) (EC 1.1.3.21), and lipase (EC 3.1.1.3), were studied. In the optimization studies for the bioactive layer components of the prepared biosensor, the optimum amounts of gelatin, bovine serum albumin (BSA), and glutaraldehyde was calculated as 1 mg/cm(2), 1 mg/cm(2), and 2.5%, respectively. Optimum pH and temperature of the reaction of biosensor were determined as 7.0 and 40 °C, respectively. Linear range of triolein for the biosensor was found from the calibration curve between several substrate concentration and Δ Current. After optimization and characterization of the biosensor, its operationability in triglycerides was also tested.
a Technology Research and Development Centre , Trakya University , Edirne , Turkey.
Alp
A
Yücel
b Faculty of Science, Department of Chemistry , Trakya University , Edirne , Turkey.
Hakkı Mevlüt
HM
Özcan
b Faculty of Science, Department of Chemistry , Trakya University , Edirne , Turkey.
Ayten
A
Sağıroğlu
D015374:Biosensing Techniques; D004566:Electrodes; D005991:Glycerol Kinase / Q000378:metabolism; D005993:Glycerolphosphate Dehydrogenase / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D008049:Lipase / Q000378:metabolism; D015203:Reproducibility of Results; D013379:Substrate Specificity; D013696:Temperature; D014280:Triglycerides / Q000032:analysis*
D014280:Triglycerides; D005993:Glycerolphosphate Dehydrogenase; C036140:L-alpha-glycerol-phosphate oxidase; D005991:Glycerol Kinase; D008049:Lipase
Amperometry; TG; glycerol kinase; glycerol-3-phosphate oxidase; lipase; multienzyme biosensor