Human intestinal mucosa-associated Lactobacillus and Bifidobacterium strains with probiotic properties modulate IL-10, IL-6 and IL-12 gene expression in THP-1 cells.
Lactobacilli and bifidobacteria are considered one of the permanent genera of the physiological human intestinal microbiota and represent an enormous pool of potential probiotic candidates. Approximately 450 isolates of presumptive Lactobacillus or Bifidobacterium strains were obtained from bioptic samples of colonic and ileal mucosa from 15 adolescents aged 12 to 18 years. On the basis of randomly amplified polymorphic DNA (RAPD)-PCR analysis, 20 strains were selected for further taxonomic classification and characterisation, as well as assessment of probiotic properties and safety. Importantly, selected strains showed the capability of colonising different parts of the intestine. The most frequently isolated species was Lactobacillus paracasei followed by Lactobacillus fermentum. The majority of isolates were susceptible to antimicrobials of human and veterinary importance, however, tetracycline and/or erythromycin resistance was observed in Lactobacillus plantarum and L. fermentum strains. Thirteen strains were able to ferment more than 19 different carbon sources and three out of five tested strains exerted antagonistic activity against several different indicator strains. Two Lactobacillus isolates (L. paracasei L350 and L. fermentum L930 bb) and one Bifidobacterium isolate (Bifidobacterium animalis subsp. animalis IM386) fulfilled in vitro selection criteria for probiotic strains and exhibited strong downregulation of pro-inflammatory cytokines IL-6 and IL-12 and upregulation of anti-inflammatory IL-10. The selected strains represent suitable candidates for further studies regarding their positive influence on host health and could play an important role in ameliorating the symptoms of inflammatory bowel diseases.
Biotechnical Faculty, Department of Animal Science, Institute of Dairy Science and Probiotics, University of Ljubljana, Groblje 3, 1230 Domžale, Slovenia Medis, Pharmaceutical Company, d.o.o., Brnčičeva 1, 1231 Ljubljana, Slovenia.
M
M
Čitar
Veterinary Faculty, National Veterinary Institute, Pri Hrastu 18, 5000 Nova Gorica, Slovenia.
B
B
Hacin
Biotechnical Faculty, Department of Animal Science, Institute of Dairy Science and Probiotics, University of Ljubljana, Groblje 3, 1230 Domžale, Slovenia.
G
G
Tompa
Medis, Pharmaceutical Company, d.o.o., Brnčičeva 1, 1231 Ljubljana, Slovenia.
M
M
Štempelj
Biotechnical Faculty, Department of Animal Science, Institute of Dairy Science and Probiotics, University of Ljubljana, Groblje 3, 1230 Domžale, Slovenia.
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I
Rogelj
Department of Pediatrics, Gastroenterology, hepatology and nutrition Unit, University Medical Centre Maribor, Ljubljanska ulica 5, 2000 Maribor, Slovenia.
J
J
Dolinšek
Biotechnical Faculty, Department of Animal Science, Chair of Genetics, Animal Biotechnology and Immunology, University of Ljubljana, Groblje 3, 1230 Domžale, Slovenia.
M
M
Narat
Biotechnical Faculty, Department of Animal Science, Institute of Dairy Science and Probiotics, University of Ljubljana, Groblje 3, 1230 Domžale, Slovenia.
B Bogovič
BB
Matijašić
D000293:Adolescent; D001644:Bifidobacterium / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D002648:Child; D005260:Female; D005767:Gastrointestinal Diseases / Q000382:microbiology; D006801:Humans; D016753:Interleukin-10 / Q000235:genetics* / Q000276:immunology; D018664:Interleukin-12 / Q000235:genetics* / Q000276:immunology; D015850:Interleukin-6 / Q000235:genetics* / Q000276:immunology; D007413:Intestinal Mucosa / Q000276:immunology / Q000382:microbiology*; D007778:Lactobacillus / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D008297:Male; D009000:Monocytes / Q000187:drug effects / Q000276:immunology*; D019936:Probiotics / Q000494:pharmacology*
C508609:IL10 protein, human; D015850:Interleukin-6; D016753:Interleukin-10; D018664:Interleukin-12
Lactobacillus, Bifidobacterium; intestinal microbiota; probiotic; safety assessment