Structure of a sialo-oligosaccharide from glycophorin in carp red blood cell membranes.
We isolated a high-purity carp glycophorin from carp erythrocyte membranes and prepared the oligosaccharide fraction from glycophorin by β-elimination [1]. The oligosaccharide fraction was separated into two components (P-1 and P-2) using a Glyco-Pak DEAE column. These O-linked oligosaccharides (P-1 and P-2) were composed of glucose, galactose, fucose, N-acetylgalactosamine and N-glycolylneuraminic acid (NeuGc). The P-1 and P-2 contained one and two NeuGc residues, respectively, and the P-1 exhibited bacteriostatic activity [1]. Using NMR and GC-MS, we determined that the structure of the bacteriostatic P-1 was NeuGcα2→6 (Fucα1→4) (Glcα1→3) Galβ1→4GalNAc-ol. This O-linked oligosaccharide was unique for a vertebrate with respect to the hexosamine and hexose linkages and its non-chain structure.
Laboratory of Quality in Marine Products, Graduate School of Bioresources, Mie University, 1577 Kurima Machiya-cho, Tsu, Mie 514-8507, Japan. aoki@bio.mie-u.ac.jp.
Takahiko
T
Aoki
Laboratory of Quality in Marine Products, Graduate School of Bioresources, Mie University, 1577 Kurima Machiya-cho, Tsu, Mie 514-8507, Japan. c.blacky@softbank.ne.jp.
Kenji
K
Chimura
Laboratory of Quality in Marine Products, Graduate School of Bioresources, Mie University, 1577 Kurima Machiya-cho, Tsu, Mie 514-8507, Japan. mie_uni1423@me.com.
Hikaru
H
Sugiura
Toray Research Centre, Inc., 1111 Tebiro, Kamakura, Kanagawa 248-8555, Japan. Yasuko_Mizuno@trc.toray.co.jp.
Yasuko
Y
Mizuno