Hierarchical Bayesian model for rare variant association analysis integrating genotype uncertainty in human sequence data.
Next-generation sequencing (NGS) has led to the study of rare genetic variants, which possibly explain the missing heritability for complex diseases. Most existing methods for rare variant (RV) association detection do not account for the common presence of sequencing errors in NGS data. The errors can largely affect the power and perturb the accuracy of association tests due to rare observations of minor alleles. We developed a hierarchical Bayesian approach to estimate the association between RVs and complex diseases. Our integrated framework combines the misclassification probability with shrinkage-based Bayesian variable selection. It allows for flexibility in handling neutral and protective RVs with measurement error, and is robust enough for detecting causal RVs with a wide spectrum of minor allele frequency (MAF). Imputation uncertainty and MAF are incorporated into the integrated framework to achieve the optimal statistical power. We demonstrate that sequencing error does significantly affect the findings, and our proposed model can take advantage of it to improve statistical power in both simulated and real data. We further show that our model outperforms existing methods, such as sequence kernel association test (SKAT). Finally, we illustrate the behavior of the proposed method using a Finnish low-density lipoprotein cholesterol study, and show that it identifies an RV known as FH North Karelia in LDLR gene with three carriers in 1,155 individuals, which is missed by both SKAT and Granvil.
Department of Public Health, Hjelt Institute, University of Helsinki, Helsinki, Finland.
Liang
L
He
Janne
J
Pitkäniemi
Antti-Pekka
AP
Sarin
Veikko
V
Salomaa
Mikko J
MJ
Sillanpää
Samuli
S
Ripatti
D000483:Alleles; D001483:Base Sequence; D001499:Bayes Theorem; D008078:Cholesterol, LDL / Q000235:genetics; D005387:Finland; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease / Q000235:genetics; D014644:Genetic Variation / Q000235:genetics*; D005838:Genotype; D006579:Heterozygote; D059014:High-Throughput Nucleotide Sequencing; D006801:Humans; D008957:Models, Genetic; D011973:Receptors, LDL / Q000235:genetics; D012984:Software; D035501:Uncertainty
D008078:Cholesterol, LDL; C573412:LDLR protein, human; D011973:Receptors, LDL
LDL-C; classification error; rare variant; shrinkage-based Bayesian variable selection