Cell-bound exopolysaccharides of Lactobacillus brevis KB290 enhance cytotoxic activity of mouse splenocytes.
OBJECTIVE
This study aimed to identify the main active component of Lactobacillus brevis KB290 (KB290) that is responsible for enhanced cell-mediated cytotoxic activity of mouse splenocytes Live KB290, a probiotic strain derived from a Japanese traditional pickle, was previously reported to modulate innate immune responses as affecting on cell-mediated cytotoxic activity of mouse splenocytes.


RESULTS
We used live KB290, heat-killed KB290, a derivative strain (Lact. brevis KB392) with different amounts of cell-bound exopolysaccharide (EPS-b), and a crude extract of EPS-b from KB290 cell surface. Female BALB/c mice were fed a diet containing 10(10) CFU live KB290, 10(10) CFU live KB392, 15 mg heat-killed KB290 or 600 μg crude extract of EPS-b for 1 day. Live KB290 (P < 0.01), heat-killed KB290 (P < 0.05) and crude EPS-b at 600 μg (P < 0.05) per mouse significantly enhanced cytotoxic activity; however, live KB392 had no effect.


CONCLUSIONS
Both live and heat-killed KB290 and crude EPS-b significantly enhanced cytotoxic activity of mouse splenocytes.


CONCLUSIONS
We demonstrated that EPS-b produced by KB290 has a critical role in enhancing cell-mediated cytotoxic activity in mouse spleen.
Nature & Wellness Research Department, Research Institute, Kagome Co. Ltd., Nasushiobara, Tochigi, Japan.
E
E
Sasaki
S
S
Suzuki
Y
Y
Fukui
N
N
Yajima
D000818:Animals; D003602:Cytotoxicity, Immunologic / Q000187:drug effects; D005260:Female; D052196:Levilactobacillus brevis; D008807:Mice, Inbred BALB C; D011135:Polysaccharides, Bacterial / Q000737:chemistry / Q000494:pharmacology*; D019936:Probiotics / Q000494:pharmacology*; D013154:Spleen / Q000276:immunology*
D011135:Polysaccharides, Bacterial
Lactobacillus brevis KB290; Lactobacillus brevis KB392; cell-bound exopolysaccharides; cell-mediated cytotoxicity; heat-killed KB290