Biological effects of soft denture reline materials on L929 cells in vitro.
Soft denture reline materials have been developed to help patients when their oral mucosa is damaged or affected due to ill-fitting dentures or post-implant surgery. Although reports have indicated that these materials leach monomers and other components that do affect their biocompatibility, there is little information on what cell molecules may be implicated in these material/tissue interactions. The biocompatibility of six soft liners (Ufi Gel P, Sofreliner S, Durabase Soft, Trusoft, Softone and Coe Comfort) was evaluated using a mouse fibroblast cell line, L929. Within 2 h of material disc preparation, each of the materials was exposed by direct contact to L929 cells for periods of 24 and 48 h. The effect of this interaction was assessed by alamarBlue assay (for cell survival). The expression of integrin α5β1 and transforming growth factor β1 was also assessed using plate assays such as enzyme-linked immunosorbent assay. Trusoft, Softone and Coe Comfort showed significantly reduced cell survival compared with the other soft lining materials at each incubation period. Furthermore, there were significant differences with these same materials in the expression of both integrin α5β1 and transforming growth factor β1. Soft liner materials may affect cell viability and cellular proteins that have important roles in wound healing and the preservation of cell viability and function in the presence of environmental challenges and stresses.
Department of Dental Materials and Prosthodontics, Araraquara Dental School, UNESP - Univ. Estadual Paulista, Araraquara, Brazil.
Carolina Al
CA
Chaves
Department of Dental Materials and Prosthodontics, Araraquara Dental School, UNESP - Univ. Estadual Paulista, Araraquara, Brazil.
Carlos E
CE
Vergani
Department of Biomaterials and Tissue Engineering, UCL Eastman Dental Institute, London, UK.
Dominique
D
Thomas
Department of Biomaterials and Tissue Engineering, UCL Eastman Dental Institute, London, UK.
Anne
A
Young
Department of Physiology and Pathology, Araraquara Dental School, UNESP - Univ. Estadual Paulista, Araraquara, Brazil.
Carlos As
CA
Costa
Department of Biomaterials and Tissue Engineering, UCL Eastman Dental Institute, London, UK ; Plymouth University Peninsula Schools of Medicine and Dentistry, Plymouth University, Plymouth, UK.
Vehid M
VM
Salih
Department of Dental Materials and Prosthodontics, Araraquara Dental School, UNESP - Univ. Estadual Paulista, Araraquara, Brazil.
Ana L
AL
Machado
Soft denture reline material; biocompatibility; fibroblast; integrin α5β1; transforming growth factor β1