title: Sugar modification inhibits autophagosome-lysosome fusion.
abstract: Autophagy is an intracellular degradation system that is mediated by orchestrated functions of membranes and proteins. A genetic screen in Caenorhabditis elegans revealed that O-linked N-acetylglucosamine modification of the SNARE protein SNAP-29 negatively regulates SNARE-dependent fusion between autophagosomes and lysosomes. This regulatory mechanism is conserved in mammals.
authors.affiliation: Department of Biochemistry and Molecular Biology, Graduate School and Faculty of Medicine, The University of Tokyo, Tokyo 113-0033, Japan.
authors.forename: Noboru
authors.initials: N
authors.lastname: Mizushima
mesh_terms: D000117:Acetylglucosamine / Q000378:metabolism*; D000818:Animals; D001343:Autophagy / Q000502:physiology*; D011992:Endosomes / Q000502:physiology*; D006801:Humans; D008247:Lysosomes / Q000502:physiology*; D008561:Membrane Fusion; D017351:N-Acetylglucosaminyltransferases / Q000378:metabolism*; D010588:Phagosomes / Q000502:physiology*; D050766:Qb-SNARE Proteins / Q000378:metabolism*; D050767:Qc-SNARE Proteins / Q000378:metabolism*
chemical_list: D050766:Qb-SNARE Proteins; D050767:Qc-SNARE Proteins; C432420:SNAP29 protein, human; D017351:N-Acetylglucosaminyltransferases; C063057:UDP-N-acetylglucosamine-peptide beta-N-acetylglucosaminyltransferase; D000117:Acetylglucosamine