title: Long-term reduction of T-cell intracellular antigens reveals a transcriptome associated with extracellular matrix and cell adhesion components.
abstract: Knockdown of T-cell intracellular antigens TIA1 and TIAR contributes to a cellular phenotype characterised by uncontrolled proliferation and tumorigenesis. Massive-scale poly(A+) RNA sequencing of TIA1 or TIAR-knocked down HeLa cells reveals transcriptome signatures comprising genes and functional categories potentially able to modulate several aspects of membrane dynamics associated with extracellular matrix and focal/cell adhesion events. The transcriptomic heterogeneity is the result of differentially expressed genes and RNA isoforms generated by alternative splicing and/or promoter usage. These results suggest a role for TIA proteins in the regulation and/or modulation of cellular homeostasis related to focal/cell adhesion, extracellular matrix and membrane and cytoskeleton dynamics.
authors.affiliation: Centro de Biología Molecular 'Severo Ochoa', Consejo Superior de Investigaciones Científicas, Universidad Autónoma de Madrid (CSIC/UAM), Madrid, Spain.
authors.forename: Mario
authors.initials: M
authors.lastname: Núñez
authors.affiliation: Centro de Biología Molecular 'Severo Ochoa', Consejo Superior de Investigaciones Científicas, Universidad Autónoma de Madrid (CSIC/UAM), Madrid, Spain.
authors.forename: Carmen
authors.initials: C
authors.lastname: Sánchez-Jiménez
authors.affiliation: Centro de Biología Molecular 'Severo Ochoa', Consejo Superior de Investigaciones Científicas, Universidad Autónoma de Madrid (CSIC/UAM), Madrid, Spain.
authors.forename: José
authors.initials: J
authors.lastname: Alcalde
authors.affiliation: Centro de Biología Molecular 'Severo Ochoa', Consejo Superior de Investigaciones Científicas, Universidad Autónoma de Madrid (CSIC/UAM), Madrid, Spain.
authors.forename: José M
authors.initials: JM
authors.lastname: Izquierdo
mesh_terms: D002448:Cell Adhesion / Q000502:physiology*; D005109:Extracellular Matrix / Q000502:physiology*; D005455:Fluorescent Antibody Technique; D020869:Gene Expression Profiling; D055785:Gene Knockdown Techniques; D015723:Gene Library; D006367:HeLa Cells; D006801:Humans; D008156:Luciferases; D008856:Microscopy, Fluorescence; D039102:Poly(A)-Binding Proteins / Q000235:genetics*; D017423:Sequence Analysis, RNA; D000074019:T-Cell Intracellular Antigen-1; D059467:Transcriptome / Q000502:physiology*
chemical_list: D039102:Poly(A)-Binding Proteins; D000074019:T-Cell Intracellular Antigen-1; C071558:TIA1 protein, human; D008156:Luciferases