title: Multifunctional peptides derived from an egg yolk protein hydrolysate: isolation and characterization.
abstract: An egg yolk protein by-product following ethanol extraction of phospholipids (YP) was hydrolyzed with pepsin to produce and identify novel peptides that revealed antioxidant, ACE inhibitory and antidiabetic (α-glucosidase and DPP-IV inhibitory) activities. The peptic hydrolysate of YP was fractionated by ion-exchange chromatography and reversed-phase high-pressure liquid chromatography. Isolated peptides were identified using mass spectrometry (MALDI-ToF) and the Mascot Search Results database. Four peptides of MW ranging from 1,210.62 to 1,677.88 Da corresponded to the fragments of Apolipoprotein B (YINQMPQKSRE; YINQMPQKSREA), Vitellogenin-2 (VTGRFAGHPAAQ) and Apovitellenin-1 (YIEAVNKVSPRAGQF). These peptides were chemically synthesized and showed antioxidant, ACE inhibitory or/and antidiabetic activities. Peptide YIEAVNKVSPRAGQF exerted the strongest ACE inhibitory activity, with IC50 = 9.4 µg/mL. The peptide YINQMPQKSRE showed the strongest DPPH free radical scavenging and DPP-IV inhibitory activities and its ACE inhibitory activity (IC50) reached 10.1 µg/mL. The peptide VTGRFAGHPAAQ revealed the highest α-glucosidase inhibitory activity (IC50 = 365.4 µg/mL). A novel nutraceutical effect for peptides from an egg yolk hydrolysate was shown.
authors.affiliation: Department of Animal Products Technology and Quality Management, Faculty of Food Science, Wrocław University of Environmental and Life Sciences, Chełmońskiego 37/41, 51-630, Wrocław, Poland, aleksandra.zambrowicz@up.wroc.pl.
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Zambrowicz
authors.forename: Marta
authors.initials: M
authors.lastname: Pokora
authors.forename: Bartosz
authors.initials: B
authors.lastname: Setner
authors.forename: Anna
authors.initials: A
authors.lastname: Dąbrowska
authors.forename: Marek
authors.initials: M
authors.lastname: Szołtysik
authors.forename: Konrad
authors.initials: K
authors.lastname: Babij
authors.forename: Zbigniew
authors.initials: Z
authors.lastname: Szewczuk
authors.forename: Tadeusz
authors.initials: T
authors.lastname: Trziszka
authors.forename: Gert
authors.initials: G
authors.lastname: Lubec
authors.forename: Józefa
authors.initials: J
authors.lastname: Chrzanowska
mesh_terms: D000818:Animals; D002645:Chickens; D004527:Egg Proteins / Q000737:chemistry*; D016166:Free Radical Scavengers / Q000737:chemistry* / Q000302:isolation & purification; D065089:Glycoside Hydrolase Inhibitors / Q000737:chemistry* / Q000302:isolation & purification; D010455:Peptides / Q000737:chemistry* / Q000302:isolation & purification; D011492:Protein Hydrolysates / Q000737:chemistry*; D012441:Saccharomyces cerevisiae / Q000201:enzymology; D029701:Saccharomyces cerevisiae Proteins / Q000037:antagonists & inhibitors / Q000737:chemistry; D000520:alpha-Glucosidases / Q000737:chemistry
chemical_list: D004527:Egg Proteins; D016166:Free Radical Scavengers; D065089:Glycoside Hydrolase Inhibitors; D010455:Peptides; D011492:Protein Hydrolysates; D029701:Saccharomyces cerevisiae Proteins; D000520:alpha-Glucosidases