title: Synthesis and characterization of amino acid containing Cu(II) chelated nanoparticles for lysozyme adsorption.
abstract: Immobilized metal ion affinity chromatography (IMAC) is a useful method for adsorption of proteins that have an affinity for transition metal ions. In this study, poly(hydroxyethyl methacrylate-methacryloyl-L-tryptophan) (PHEMATrp) nanoparticles were prepared by surfactant free emulsion polymerization. Then, Cu(II) ions were chelated on the PHEMATrp nanoparticles to be used in lysozyme adsorption studies in batch system. The maximum lysozyme adsorption capacity of the PHEMATrp nanoparticles was found to be 326.9 mg/g polymer at pH 7.0. The nonspecific lysozyme adsorption onto the PHEMA nanoparticles was negligible. In terms of protein desorption, it was observed that adsorbed lysozyme was readily desorbed in medium containing 1.0 M NaCl. The results showed that the metal-chelated PHEMATrp nanoparticles can be considered as a good adsorbent for lysozyme purification.
authors.affiliation: Hacettepe University, Department of Chemistry, Ankara, Turkey.
authors.forename: Gözde
authors.initials: G
authors.lastname: Baydemir
authors.affiliation: Hacettepe University, Department of Chemistry, Ankara, Turkey.
authors.forename: Müge
authors.initials: M
authors.lastname: Andaç
authors.affiliation: Hacettepe University, Department of Chemistry, Ankara, Turkey.
authors.forename: Ali
authors.initials: A
authors.lastname: Derazshamshir
authors.affiliation: Adnan Menderes University, Department of Chemistry, Aydın, Turkey.
authors.forename: Deniz Aktaş
authors.initials: DA
authors.lastname: Uygun
authors.affiliation: Ege University, Department of Biochemistry, İzmir, Turkey.
authors.forename: Emir
authors.initials: E
authors.lastname: Özçalışkan
authors.affiliation: Ege University, Department of Biochemistry, İzmir, Turkey. Electronic address: sinanakgol@yahoo.co.uk.
authors.forename: Sinan
authors.initials: S
authors.lastname: Akgöl
authors.affiliation: Hacettepe University, Department of Chemistry, Ankara, Turkey.
authors.forename: Adil
authors.initials: A
authors.lastname: Denizli
mesh_terms: D000327:Adsorption; D000596:Amino Acids / Q000737:chemistry*; D000818:Animals; D002614:Chelating Agents / Q000737:chemistry*; D002645:Chickens; D003300:Copper / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D009113:Muramidase / Q000302:isolation & purification*; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D054118:Nanospheres; D009994:Osmolar Concentration; D011102:Polyhydroxyethyl Methacrylate / Q000737:chemistry; D012492:Salts / Q000737:chemistry; D012996:Solutions; D013696:Temperature; D014364:Tryptophan / Q000737:chemistry
chemical_list: D000596:Amino Acids; D002614:Chelating Agents; D012492:Salts; D012996:Solutions; D011102:Polyhydroxyethyl Methacrylate; D003300:Copper; D014364:Tryptophan; D009113:Muramidase