title: Complete genome sequence of a proposed new tymovirus, tomato blistering mosaic virus.
abstract: In a previous work, a distinct tymovirus infecting tomato plants in Brazil was reported and tentatively named tomato blistering mosaic virus (ToBMV). In this study, the complete genome sequence of ToBMV was determined and shown to have a size of 6277 nucleotides and three ORFs: ORF 1 encodes the replication-complex polyprotein, ORF 2 the movement protein, and ORF 3 the coat protein. The cleavage sites of the replication-complex polyprotein (GS/LP and VAG/QSP) of ToBMV were predicted by alignment analysis of amino acid sequences of other tymoviruses. In the phylogenetic tree, ToBMV clustered with the tymoviruses that infect solanaceous hosts.
authors.affiliation: Universidade Estadual do Piauí, Teresina, Piauí, Brazil.
authors.forename: Cícero
authors.initials: C
authors.lastname: Nicolini
authors.forename: Alice Kazuko
authors.initials: AK
authors.lastname: Inoue-Nagata
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Nagata
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002874:Chromosome Mapping; D016679:Genome, Viral / Q000235:genetics; D018551:Solanum lycopersicum / Q000821:virology*; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology*; D012367:RNA, Viral / Q000235:genetics; D016415:Sequence Alignment; D017423:Sequence Analysis, RNA; D017806:Tymovirus / Q000145:classification / Q000235:genetics*
chemical_list: D012367:RNA, Viral