title: [Allele-specific PCR and its application in forensic science].
abstract: Allele-specific polymerase chain reaction (AS-PCR) is a technique based on allele-specific primers, which can be used to analyze single nucleotide polymorphism (SNP) effectively including the transition, transversion and insertion/deletion polymorphism and has been exploited in the study of diseases research, molecular diagnosis, and forensic biological evidence. The article systematically reviews the principle, the detection methods, improvement of AS-PCR, and its research updates in the fields of autosome, Y chromosome and mitochondrial SNP, as well as its application in forensic science.
authors.forename: Yan-chai
authors.initials: YC
authors.lastname: Nie
authors.forename: Bin
authors.initials: B
authors.lastname: Wang
authors.forename: Zi-qin
authors.initials: ZQ
authors.lastname: Zhao
authors.forename: Huai-gu
authors.initials: HG
authors.lastname: Zhou
mesh_terms: D000483:Alleles; D017931:DNA Primers; D044707:Forensic Sciences; D006801:Humans; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D020641:Polymorphism, Single Nucleotide
chemical_list: D017931:DNA Primers