title: Cyclic nucleotide gated channel 10 negatively regulates salt tolerance by mediating Na+ transport in Arabidopsis.
abstract: A number of cyclic nucleotide gated channel (CNGC) genes have been identified in plant genomes, but their functions are mainly undefined. In this study, we identified the role of CNGC10 in the response of Arabidopsis thaliana to salt stress. The cngc10 T-DNA insertion mutant showed greater tolerance to salt than wild-type A. thaliana during seed germination and seedling growth. The cngc10 mutant accumulated less Na(+) and K(+), but not less Ca(2+), in shoots in response to salt stress. By contrast, overexpression of CNGC10 resulted in greater sensitivity to salt stress, and complementation of this gene recovered salt sensitivity. In response to salt stress, heterologous expression of CNGC10 in the Na(+) sensitive yeast mutant strain B31 inhibited growth due to accumulation of Na(+) at a rate greater than that of yeast transformed with an empty vector. Quantitative RT-PCR analysis demonstrated that CNGC10 was expressed mainly in roots and flowers. GUS analysis of a root cross section indicated that CNGC10 was expressed mainly in the endodermis and epidermis. Furthermore, the expression of CNGC10 in roots was dramatically inhibited by exposure to 200 mM NaCl for 6 h. These data suggest that CNGC10 negatively regulates salt tolerance in A. thaliana and may be involved in mediating Na(+) transport.
authors.affiliation: College of Life Sciences, State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, 210095, People's Republic of China.
authors.forename: Yakang
authors.initials: Y
authors.lastname: Jin
authors.forename: Wen
authors.initials: W
authors.lastname: Jing
authors.forename: Qun
authors.initials: Q
authors.lastname: Zhang
authors.forename: Wenhua
authors.initials: W
authors.lastname: Zhang
mesh_terms: D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics / Q000502:physiology*; D029681:Arabidopsis Proteins / Q000378:metabolism*; D001692:Biological Transport / Q000187:drug effects; D054815:Cyclic Nucleotide-Gated Cation Channels / Q000378:metabolism*; D015536:Down-Regulation / Q000187:drug effects; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D055786:Gene Knockout Techniques; D005816:Genetic Complementation Test; D007477:Ions; D009154:Mutation / Q000235:genetics; D010641:Phenotype; D018520:Plant Shoots / Q000187:drug effects / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics; D055049:Salt Tolerance / Q000187:drug effects / Q000235:genetics; D036226:Seedlings / Q000187:drug effects / Q000502:physiology; D012964:Sodium / Q000378:metabolism*; D012965:Sodium Chloride / Q000494:pharmacology; D013312:Stress, Physiological / Q000187:drug effects; D014170:Transformation, Genetic
chemical_list: D029681:Arabidopsis Proteins; C525437:CNGC10 protein, Arabidopsis; D054815:Cyclic Nucleotide-Gated Cation Channels; D007477:Ions; D012965:Sodium Chloride; D012964:Sodium