title: Supercritical CO₂ extract and essential oil of aerial part of Ledum palustre L.--Chemical composition and anti-inflammatory activity.
abstract: The anti-inflammatory activity of two extracts from the aerial parts of Ledum palustre has been reported. The volatile oil was obtained by supercritical fluid extraction (SFE) and the essential oil by hydrodistillation (HD). The oils were analysed by gas chromatography-mass spectrometry to monitor their composition. Both extracts shared as main compound (41.0-43.4%) ledol (23.3-26.7%) and ascaridole (15.1-4.5%). The anti-inflammatory activity was evaluated by the subcutaneous carrageenan injection-induced hind paw oedema. The treated animals received essential oil (SFE and HD), the reference group received ketoprofen or piroxicam and the control group received NaCl 0.9%. A statistical analysis was performed by the Student t-test. The results show that L. palustre essential oil enhanced a significant inhibition of oedema (50-73%) for HD oil and (52-80%) for SFE oil. These results were similar to those obtained with piroxicam (70%) and ketoprofen (55%).
authors.affiliation: a Laboratory of Pharmacology , Faculty of Medicine, University of Monastir , 5019 Monastir , Tunisia.
authors.forename: Sameh
authors.initials: S
authors.lastname: Baananou
authors.forename: Edita
authors.initials: E
authors.lastname: Bagdonaite
authors.forename: Bruno
authors.initials: B
authors.lastname: Marongiu
authors.forename: Alessandra
authors.initials: A
authors.lastname: Piras
authors.forename: Silvia
authors.initials: S
authors.lastname: Porcedda
authors.forename: Danilo
authors.initials: D
authors.lastname: Falconieri
authors.forename: Naceur A
authors.initials: NA
authors.lastname: Boughattas
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D025924:Chromatography, Supercritical Fluid; D000081005:Cyclohexane Monoterpenes; D004487:Edema / Q000188:drug therapy; D008401:Gas Chromatography-Mass Spectrometry; D029794:Ledum / Q000737:chemistry*; D008097:Lithuania; D039821:Monoterpenes / Q000737:chemistry / Q000302:isolation & purification; D009822:Oils, Volatile / Q000737:chemistry*; D010545:Peroxides / Q000737:chemistry / Q000302:isolation & purification; D035261:Plant Components, Aerial / Q000737:chemistry*; D010938:Plant Oils / Q000737:chemistry*; D017208:Rats, Wistar; D012717:Sesquiterpenes / Q000737:chemistry / Q000302:isolation & purification
chemical_list: D000893:Anti-Inflammatory Agents; D000081005:Cyclohexane Monoterpenes; D039821:Monoterpenes; D009822:Oils, Volatile; D010545:Peroxides; D010938:Plant Oils; D012717:Sesquiterpenes; C043372:ascaridole; C421806:ledol
keywords: Ledum palustre; anti-inflammatory activity; essential oil