title: Development of 3-alkyl-6-methoxy-7-hydroxy-chromones (AMHCs) from natural isoflavones, a new class of fluorescent scaffolds for biological imaging.
abstract: Starting from 7-hydroxyisoflavones, we developed a new class of fluorescent scaffolds, 3-alkyl-6-methoxy-7-hydroxy-chromones (AMHCs, MW∼ 205.19, λab∼ 350 nm, λem∼ 450 nm) via a trial and error process. AMHCs have the advantages of being a small molecular moiety, having strong fluorescence in basic buffers, reasonable solubility and stability, non-toxicity, and are conveniently linked to pharmacophores. AMHCs were successfully used in fluorescence microscopy imaging of cells and tissues.
authors.affiliation: State Key Laboratory of Bioactive Substances and Function of Natural Medicine, Institute of Materia Medica, Peking Union Medical College and Chinese Academy of Medical Sciences, 1 Xiannongtan Street, Beijing, 100050, China. yindali@imm.ac.cn hcui@imm.ac.cn.
authors.forename: Jianzhuang
authors.initials: J
authors.lastname: Miao
authors.forename: Huaqing
authors.initials: H
authors.lastname: Cui
authors.forename: Jing
authors.initials: J
authors.lastname: Jin
authors.forename: Fangfang
authors.initials: F
authors.lastname: Lai
authors.forename: Hui
authors.initials: H
authors.lastname: Wen
authors.forename: Xiang
authors.initials: X
authors.lastname: Zhang
authors.forename: Gian Filippo
authors.initials: GF
authors.lastname: Ruda
authors.forename: Xiaoguang
authors.initials: X
authors.lastname: Chen
authors.forename: Dali
authors.initials: D
authors.lastname: Yin
mesh_terms: D000818:Animals; D001688:Biological Products / Q000737:chemistry*; D002867:Chromones / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D056945:Hep G2 Cells; D006801:Humans; D007529:Isoflavones / Q000737:chemistry*; D051379:Mice; D061848:Optical Imaging / Q000379:methods*; D012995:Solubility
chemical_list: C582365:7-hydroxyisoflavone; D001688:Biological Products; D002867:Chromones; D005456:Fluorescent Dyes; D007529:Isoflavones