title: Antioxidative activities and active compounds of extracts from Catalpa plant leaves.
abstract: In order to screen the Catalpa plant with high antioxidant activity and confirm the corresponding active fractions from Catalpa ovata G. Don, C. fargesii Bur., and C. bungei C. A. Mey., total flavonoid contents and antioxidant activities of the extracts/fractions of Catalpa plant leaves were determined. The determined total flavonoid content and antioxidant activity were used as assessment criteria. Those compounds with antioxidant activity were isolated with silica gel column chromatography and ODS column chromatography. Our results showed that the total flavonoid content in C. bungei C. A. Mey. (30.07 mg/g · DW) was the highest, followed by those in C. fargesii Bur. (25.55 mg/g · DW) and C. ovata G. Don (24.96 mg/g · DW). According to the determination results of total flavonoid content and antioxidant activity in 3 clones of leaves of C. bungei C. A. Mey., the total flavonoid content and antioxidant activity in crude extracts from C. bungei C. A. Mey. 6 (CA6) leaves were the highest. Moreover, the results showed that the total flavonoid content and antioxidant activities of ethyl acetate (EA) fraction in ethanol crude extracts in CA6 leaves were the highest, followed by n-butanol, petroleum ether (PE), and water fractions. Two flavonoid compounds with antioxidant activity were firstly isolated based on EA fraction. The two compounds were luteolin (1) and apigenin (2), respectively.
authors.affiliation: College of Forestry, Northwest A&F University, Yangling, Shaanxi 712100, China.
authors.forename: Hongyu
authors.initials: H
authors.lastname: Xu
authors.affiliation: College of Life Sciences, Northwest A&F University, Yangling, Shaanxi 712100, China.
authors.forename: Gege
authors.initials: G
authors.lastname: Hu
authors.affiliation: College of Life Sciences, Northwest A&F University, Yangling, Shaanxi 712100, China.
authors.forename: Juane
authors.initials: J
authors.lastname: Dong
authors.affiliation: College of Forestry, Northwest A&F University, Yangling, Shaanxi 712100, China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Wei
authors.affiliation: Key Laboratory of Coastal Biology & Bioresources Utilization, Yantai Institute of Coastal Zone Research (YIC), Chinese Academy of Sciences (CAS), Yantai 264003, China ; Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China.
authors.forename: Hongbo
authors.initials: H
authors.lastname: Shao
authors.affiliation: College of Life Sciences, Northwest A&F University, Yangling, Shaanxi 712100, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Lei
mesh_terms: D020001:1-Butanol; D000085:Acetates; D000473:Alkanes; D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification*; D047310:Apigenin / Q000737:chemistry / Q000302:isolation & purification*; D029402:Bignoniaceae / Q000737:chemistry*; D001713:Biphenyl Compounds / Q000037:antagonists & inhibitors; D000431:Ethanol; D017665:Hydroxyl Radical / Q000037:antagonists & inhibitors; D047311:Luteolin / Q000737:chemistry / Q000302:isolation & purification*; D010851:Picrates / Q000037:antagonists & inhibitors; D010936:Plant Extracts / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry*; D012997:Solvents
chemical_list: D000085:Acetates; D000473:Alkanes; D000975:Antioxidants; D001713:Biphenyl Compounds; D010851:Picrates; D010936:Plant Extracts; D012997:Solvents; D017665:Hydroxyl Radical; D000431:Ethanol; C007650:ethyl acetate; D047310:Apigenin; D020001:1-Butanol; C004931:1,1-diphenyl-2-picrylhydrazyl; D047311:Luteolin; C004544:naphtha