title: The Shigella flexneri type 3 secretion system is required for tyrosine kinase-dependent protrusion resolution, and vacuole escape during bacterial dissemination.
abstract: Shigella flexneri is a human pathogen that triggers its own entry into intestinal cells and escapes primary vacuoles to gain access to the cytosolic compartment. As cytosolic and motile bacteria encounter the cell cortex, they spread from cell to cell through formation of membrane protrusions that resolve into secondary vacuoles in adjacent cells. Here, we examined the roles of the Type 3 Secretion System (T3SS) in S. flexneri dissemination in HT-29 intestinal cells infected with the serotype 2a strain 2457T. We generated a 2457T strain defective in the expression of MxiG, a central component of the T3SS needle apparatus. As expected, the ΔmxiG strain was severely affected in its ability to invade HT-29 cells, and expression of mxiG under the control of an arabinose inducible expression system (ΔmxiG/pmxiG) restored full infectivity. In this experimental system, removal of the inducer after the invasion steps (ΔmxiG/pmxiG (Ara withdrawal)) led to normal actin-based motility in the cytosol of HT-29 cells. However, the time spent in protrusions until vacuole formation was significantly increased. Moreover, the number of formed protrusions that failed to resolve into vacuoles was also increased. Accordingly, the ΔmxiG/pmxiG (Ara withdrawal) strain failed to trigger tyrosine phosphorylation in membrane protrusions, a signaling event that is required for the resolution of protrusions into vacuoles. Finally, the ΔmxiG/pmxiG (Ara withdrawal) strain failed to escape from the formed secondary vacuoles, as previously reported in non-intestinal cells. Thus, the T3SS system displays multiple roles in S. flexneri dissemination in intestinal cells, including the tyrosine kinase signaling-dependent resolution of membrane protrusions into secondary vacuoles, and the escape from the formed secondary vacuoles.
authors.affiliation: Department of Microbial Pathogenesis, Yale School of Medicine, Boyer Center for Molecular Medicine, New Haven, Connecticut, United States of America.
authors.forename: Carole J
authors.initials: CJ
authors.lastname: Kuehl
authors.affiliation: Department of Microbial Pathogenesis, Yale School of Medicine, Boyer Center for Molecular Medicine, New Haven, Connecticut, United States of America.
authors.forename: Ana-Maria
authors.initials: AM
authors.lastname: Dragoi
authors.affiliation: Department of Microbial Pathogenesis, Yale School of Medicine, Boyer Center for Molecular Medicine, New Haven, Connecticut, United States of America.
authors.forename: Hervé
authors.initials: H
authors.lastname: Agaisse
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D022081:Cell Surface Extensions / Q000502:physiology*; D017931:DNA Primers / Q000235:genetics; D004405:Dysentery, Bacillary / Q000378:metabolism / Q000503:physiopathology*; D005455:Fluorescent Antibody Technique; D019073:HT29 Cells; D006801:Humans; D010766:Phosphorylation; D011505:Protein-Tyrosine Kinases / Q000378:metabolism; D012763:Shigella flexneri / Q000378:metabolism*; D000069296:Type III Secretion Systems / Q000378:metabolism*; D014617:Vacuoles / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D017931:DNA Primers; D000069296:Type III Secretion Systems; D011505:Protein-Tyrosine Kinases