title: Fc-optimized antibodies quickly pull the trigger.
abstract: In this issue of Blood, Romain et al demonstrate that natural killer (NK) cell–mediated killing of tumor cells coated with the Fc-optimized CD33 antibody DLE-HuM195 reveals a distinct kinetic profile. The presented work gives important novel insights into the mechanism of effector cell–mediated target cell killing triggered by Fc-engineered antibodies and explains how they achieve a higher antibody-dependent cell-mediated cytotoxicity (ADCC) potency than native immunoglobulin G1 (IgG1) antibodies. Using time-lapse imaging microscopy in nanowell grids (TIMING), the authors were able to demonstrate at the single-cell level that antibody Fc engineering improves frequency and promotes kinetic boosting of serial killing mediated by NK cells.
authors.affiliation: CHRISTIAN-ALBRECHTS-UNIVERSITY KIEL.
authors.forename: Christian
authors.initials: C
authors.lastname: Kellner
authors.affiliation: CHRISTIAN-ALBRECHTS-UNIVERSITY KIEL.
authors.forename: Matthias
authors.initials: M
authors.lastname: Peipp
mesh_terms: D000920:Antibody-Dependent Cell Cytotoxicity; D006801:Humans; D007141:Immunoglobulin Fc Fragments / Q000276:immunology*; D007074:Immunoglobulin G / Q000276:immunology*; D007694:Killer Cells, Natural / Q000276:immunology*
chemical_list: D007141:Immunoglobulin Fc Fragments; D007074:Immunoglobulin G