title: Effect of ethanol and methanol on growth of ruminal bacteria Selenomonas ruminantium and Butyrivibrio fibrisolvens.
abstract: The effect of ethanol and methanol on growth of several ruminal bacterial strains was examined. Ethanol concentrations as low as 0.2% had a significant, but moderate, inhibitory effect on lag time or growth over time and 3.3% ethanol significantly inhibited maximum optical density obtained by both Selenomonas ruminantium and Butyrivibrio fibrisolvens. Little growth of either strain occurred at 10% ethanol concentrations. Methanol concentrations below 0.5% had little effect on either growth or maximum optical density of Selenomonas ruminantium whereas methanol concentrations below 3.3% had little effect on growth or maximum optical density of Butyrivibrio fibrisolvens. Higher methanol concentrations increasingly inhibited growth of both strains and no growth occurred at a 10% methanol concentration. Concentrations of ethanol or methanol used to add hydrophobic compounds to culture media should be kept below 1%.
authors.affiliation: a Department of Animal Sciences , Purdue University , West Lafayette , Indiana , USA.
authors.forename: J A
authors.initials: JA
authors.lastname: Patterson
authors.forename: S C
authors.initials: SC
authors.lastname: Ricke
mesh_terms: D000818:Animals; D045856:Butyrivibrio / Q000187:drug effects* / Q000254:growth & development; D003470:Culture Media; D004305:Dose-Response Relationship, Drug; D000431:Ethanol / Q000494:pharmacology*; D000432:Methanol / Q000494:pharmacology*; D012417:Rumen / Q000382:microbiology*; D020357:Selenomonas / Q000187:drug effects* / Q000254:growth & development
chemical_list: D003470:Culture Media; D000431:Ethanol; D000432:Methanol
keywords: Ethanol; bacteria; growth; methanol; ruminal; toxicity