title: Retrospective analysis of nuclear protein in testis (NUT) midline carcinoma in the upper aerodigestive tract and mediastinum.
abstract: OBJECTIVE
  Nuclear protein in testis (NUT) midline carcinoma (NMC) is a very aggressive tumor with limited survival, recently recognized as a subset of poorly differentiated squamous cell carcinoma. A simple chromosomal translocation results in NUT overexpression and malignant transformation. This study used immunohistochemistry to retrospectively diagnose and characterize NMC cases.
  
  
  METHODS
  Immunoperoxidase staining was performed according to a standard protocol and interpreted independently by two pathologists. Scores were based on nuclear staining with monoclonal NUT antibody (C52B1) in the tumor cells.
  
  
  RESULTS
  Fifty-one poorly differentiated carcinoma cases with material available for testing were retrieved. Average patient age was 54.9 years (range: 16-82), with 20 women and 31 men. A single NMC case (2%) was retrospectively diagnosed in a 26-year-old man with a left maxillary sinus/nasal cavity tumor; he died of his disease 18 months after presentation, despite treatment.
  
  
  CONCLUSIONS
  These results support inclusion of NUT antibody in diagnostic immunohistochemical panels for poorly differentiated carcinomas of the upper aerodigestive tract.
authors.affiliation: Department of Pathology and Laboratory Medicine, Emory University School of Medicine, Emory University, Atlanta, GA, USA; Department of Oral Diagnostic Sciences, Nova Southeastern University, College of Dental Medicine, Emory University, Atlanta, GA, USA. Electronic address: Lsolomon1@nova.edu.
authors.forename: Lynn W
authors.initials: LW
authors.lastname: Solomon
authors.affiliation: Department of Pathology and Laboratory Medicine, Emory University School of Medicine, Emory University, Atlanta, GA, USA.
authors.forename: Kelly R
authors.initials: KR
authors.lastname: Magliocca
authors.affiliation: Department of Pathology and Laboratory Medicine, Emory University School of Medicine, Emory University, Atlanta, GA, USA.
authors.forename: Cynthia
authors.initials: C
authors.lastname: Cohen
authors.affiliation: Department of Pathology and Laboratory Medicine, Emory University School of Medicine, Emory University, Atlanta, GA, USA; Department of Otolaryngology Head and Neck Surgery, Emory University School of Medicine, Emory University, Atlanta, GA, USA.
authors.forename: Susan
authors.initials: S
authors.lastname: Müller
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002294:Carcinoma, Squamous Cell / Q000473:pathology*; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D008297:Male; D008479:Mediastinal Neoplasms / Q000473:pathology*; D008875:Middle Aged; D009363:Neoplasm Proteins; D009687:Nuclear Proteins / Q000032:analysis*; D015513:Oncogene Proteins / Q000032:analysis*; D010039:Otorhinolaryngologic Neoplasms / Q000473:pathology*; D012189:Retrospective Studies; D012307:Risk Factors
chemical_list: C000630260:NUTM1 protein, human; D009363:Neoplasm Proteins; D009687:Nuclear Proteins; D015513:Oncogene Proteins