title: Novel L-lactic acid biosensors based on conducting polypyrrole-block copolymer nanoparticles.
abstract: The development of advanced nanomaterials for the highly efficient electrical detection of biological species has attracted great attention. Here, novel polypyrrole-Pluronic F127 nanoparticles (PPy-F127 NPs) with conducting and biocompatibility properties were synthesized and used to construct a L-lactic acid biosensor that could be applied in biochemical assays. The PPy-F127 NPs were characterized by transmission electron microscopy (TEM), elemental analysis and UV-vis spectroscopy. Lactate oxidase (LOx) structure variation on the PPy-F127 NPs was investigated by circular dichroism (CD). The cyclic voltammetric results indicated that LOx immobilized on the PPy-F127 NPs exhibited direct electron transfer reaction with a formal potential value (E(0)') of 0.154 V vs. SCE. Moreover, the biosensor had good electrocatalytic activity toward L-lactic acid with a wide linear range (0.015-37.5 mM) and a low detection limit of 0.0088 mM. The regression equation was I (μA) = 0.02353c (mM) + 1.4135 (R(2) = 0.9939). The L-lactic acid biosensor had a good anti-interference property towards uric acid (UA), ascorbic acid (AA), glucose and cysteine. The idea and method provide a promising platform for the rapid development of biosensors that can be used in the detection of biological species.
authors.affiliation: Institute of Agricultural Products Processing, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, PR China.
authors.forename: Chong
authors.initials: C
authors.lastname: Sun
authors.forename: Daoying
authors.initials: D
authors.lastname: Wang
authors.forename: Muhan
authors.initials: M
authors.lastname: Zhang
authors.forename: Yanxiu
authors.initials: Y
authors.lastname: Ni
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Shen
authors.forename: Youchao
authors.initials: Y
authors.lastname: Song
authors.forename: Zhiming
authors.initials: Z
authors.lastname: Geng
authors.forename: Weimin
authors.initials: W
authors.lastname: Xu
authors.forename: Fang
authors.initials: F
authors.lastname: Liu
authors.forename: Chun
authors.initials: C
authors.lastname: Mao
mesh_terms: D000818:Animals; D001205:Ascorbic Acid / Q000032:analysis; D015374:Biosensing Techniques / Q000379:methods*; D002942:Circular Dichroism; D003545:Cysteine / Q000032:analysis; D004563:Electrochemistry; D004566:Electrodes; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism; D005947:Glucose / Q000032:analysis; D019344:Lactic Acid / Q000032:analysis*; D057230:Limit of Detection; D046529:Microscopy, Electron, Transmission; D006899:Mixed Function Oxygenases / Q000737:chemistry / Q000378:metabolism; D018482:Muscle, Skeletal / Q000378:metabolism*; D053758:Nanoparticles / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D011758:Pyrroles / Q000737:chemistry*; D013552:Swine; D014527:Uric Acid / Q000032:analysis
chemical_list: D004800:Enzymes, Immobilized; D011108:Polymers; D011758:Pyrroles; D014527:Uric Acid; C067635:polypyrrole; D019344:Lactic Acid; D006899:Mixed Function Oxygenases; C021497:lactate 2-monooxygenase; D005947:Glucose; D003545:Cysteine; D001205:Ascorbic Acid