title: Biotin-streptavidin-amplified real-time immune-PCR assay for detecting dimethyl phthalate in beverage and drinking water samples.
abstract: Dimethyl phthalate (DMP), one family of the phthalic acid diesters (PAEs), is an increasing widely used plasticizer. A sensitive and high-throughput direct competitive biotin-streptavidin-amplified system based on real-time immune-PCR (BA-rt-IPCR) techniques was developed for detecting DMP in beverage and drinking water samples. In our assay, we selected dimethyl 4-aminophthalate as the optimal DMP hapten to prepare high titer of rabbit polyclonal anti-DMP antibodies (pAb-DMP). Under the optimized conditions, the proposed method was used to detect DMP with a linearity range from 10 to 100 ng L(-1), and the limit of detection (LOD) was 1.98 pg L(-1). Finally, the results about DMP in beverage and drinking water samples were consistent with those using gas chromatography-mass spectrometry (GC-MS), which proved that the proposed immunoassay for detecting DMP in the environment was accurate, reliably rapid, and receptive.
authors.affiliation: School of Environmental Science and Engineering, Shanghai Jiao Tong University, 800 Dong Chuan Road, Shanghai, 200240, China.
authors.forename: Ruiyan
authors.initials: R
authors.lastname: Sun
authors.forename: Huisheng
authors.initials: H
authors.lastname: Zhuang
mesh_terms: D001628:Beverages / Q000032:analysis*; D001710:Biotin / Q000737:chemistry; D060766:Drinking Water / Q000032:analysis* / Q000737:chemistry; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005504:Food Analysis / Q000295:instrumentation / Q000379:methods; D057230:Limit of Detection; D010795:Phthalic Acids / Q000032:analysis*; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D019809:Streptavidin / Q000737:chemistry; D014874:Water Pollutants, Chemical / Q000032:analysis*
chemical_list: D060766:Drinking Water; D010795:Phthalic Acids; D014874:Water Pollutants, Chemical; C024629:dimethyl phthalate; D001710:Biotin; D019809:Streptavidin