title: Growth suppression of colorectal cancer by plant-derived multiple mAb CO17-1A × BR55 via inhibition of ERK1/2 phosphorylation.
abstract: We have generated the transgenic Tabaco plants expressing multiple monoclonal antibody (mAb) CO7-1A × BR55 by cross-pollinating with mAb CO17-1A and mAb BR55. We have demonstrated the anti-cancer effect of plant-derived multiple mAb CO17-1A × BR55. We find that co-treatment of colorectal mAbs (anti-epithelial cellular adhesion molecule (EpCAM), plant-derived monoclonal antibody (mAb(P)) CO17-1A and mAb(P) CO17-1A × BR55) with RAW264.7 cells significantly inhibited the cell growth in SW620 cancer cells. In particular, multi mAb(P) CO17-1A × BR55 significantly and efficiently suppressed the growth of SW620 cancer cells compared to another mAbs. Apoptotic death-positive cells were significantly increased in the mAb(P) CO17-1A × BR55-treated. The mAb(P) CO17-1A × BR55 treatment significantly decreased the expression of B-Cell lymphoma-2 (BCl-2), but the expression of Bcl-2-associated X protein (Bax), and cleaved caspase-3 were markedly increased. In vivo, the mAb(P) CO17-1A × BR55 significantly and efficiently inhibited the growth of colon tumors compared to another mAbs. The apoptotic cell death and inhibition of pro-apoptotic proteins expression were highest by treatment with mAb(P) CO17-1A × BR55. In addition, the mAb(P) CO17-1A × BR55 significantly inhibited the extracellular signal-regulated kinase 1 and 2 (ERK1/2) phosphorylation in cancer cells and tumors. Therefore, this study results suggest that multiple mAb(P) CO17-1A × BR55 has a significant effect on apoptosis-mediated anticancer by suppression of ERK1/2 phosphorylation in colon cancer compared to another mAbs. In light of these results, further clinical investigation should be conducted on mAb(P) CO17-1A × BR55 to determine its possible chemopreventive and/or therapeutic efficacy against human colon cancer.
authors.affiliation: Institute of Glycoscience, Wonkwang University, Iksan, Jeonbuk 570-749, Korea. velvety7@nate.com.
authors.forename: Dong Hoon
authors.initials: DH
authors.lastname: Kwak
authors.affiliation: Department of Biological Science, College of Natural Sciences, Institute of Biotechnology Wonkwang University, Iksan, Jeonbuk 570-749, Korea. strozzi37@gmail.com.
authors.forename: Ghislain
authors.initials: G
authors.lastname: Moussavou
authors.affiliation: Department of Biological Science, College of Natural Sciences, Institute of Biotechnology Wonkwang University, Iksan, Jeonbuk 570-749, Korea. oooe64@naver.com.
authors.forename: Ju Hyoung
authors.initials: JH
authors.lastname: Lee
authors.affiliation: Department of Biological Science, College of Natural Sciences, Institute of Biotechnology Wonkwang University, Iksan, Jeonbuk 570-749, Korea. akewhen@gmail.com.
authors.forename: Sung Youn
authors.initials: SY
authors.lastname: Heo
authors.affiliation: Department of Medicine, Medical Research Institute, College of Medicine Chung-Ang University, Heukseok-ro 84, Seoul 156-756, Korea. ksko@cau.ac.kr.
authors.forename: Kisung
authors.initials: K
authors.lastname: Ko
authors.affiliation: Department of Agrofood Resources, National Academy of Agricultural Science, RDA, Suwon 441-853, Korea. kah366@korea.kr.
authors.forename: Kyung-A
authors.initials: KA
authors.lastname: Hwang
authors.affiliation: Department of Clinical Laboratory Science, Wonkwang Health Science University, Iksan zipcode, Korea. sjjei@wu.ac.kr.
authors.forename: Seung-Joo
authors.initials: SJ
authors.lastname: Jekal
authors.affiliation: Institute of Glycoscience, Wonkwang University, Iksan, Jeonbuk 570-749, Korea. ykchoo@wonkwang.ac.kr.
authors.forename: Young-Kug
authors.initials: YK
authors.lastname: Choo
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000235:genetics / Q000627:therapeutic use*; D000951:Antigens, Neoplasm / Q000235:genetics / Q000276:immunology*; D002460:Cell Line; D045744:Cell Line, Tumor; D003106:Colon / Q000276:immunology / Q000473:pathology; D015179:Colorectal Neoplasms / Q000276:immunology / Q000473:pathology / Q000628:therapy*; D006801:Humans; D007167:Immunotherapy; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D019950:Mitogen-Activated Protein Kinase 1 / Q000276:immunology*; D048052:Mitogen-Activated Protein Kinase 3 / Q000276:immunology*; D010766:Phosphorylation; D030821:Plants, Genetically Modified / Q000235:genetics; D012007:Rectum / Q000276:immunology / Q000473:pathology; D014026:Nicotiana / Q000235:genetics
chemical_list: D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; C050928:carcinoma-associated antigen 17-1A; D019950:Mitogen-Activated Protein Kinase 1; D048052:Mitogen-Activated Protein Kinase 3