title: Depolarization affects the lateral microdomain structure of yeast plasma membrane.
abstract: We report the transmembrane voltage-induced lateral reorganization of highly-ordered lipid microdomains in the plasma membrane of living Saccharomyces cerevisiae. Using trans-parinaric acid (all-trans-9,11,13,15-octadecatetraenoic acid) as a probe of lipid order and different methods of membrane depolarization, we found that depolarization always invokes a significant reduction in the amount of gel-like microdomains in the membrane. Different depolarization mechanisms, including the application of ionophores, cell depolarization by an external electric field, depolarization by proton/hexose co-transport facilitated by HUP1 protein and a reduction of membrane potential caused by compromised respiration efficiency, yielded the same results independently of the yeast strain used. The data suggest that the voltage-induced reorganization of lateral membrane structure could play significant role in fast cellular response to acute stress conditions, as well as in other membrane microdomain-related regulatory mechanisms.
authors.affiliation: Faculty of Mathematics and Physics, Charles University, Prague, Czech Republic.
authors.forename: Petr
authors.initials: P
authors.lastname: Herman
authors.forename: Jaroslav
authors.initials: J
authors.lastname: Vecer
authors.forename: Miroslava
authors.initials: M
authors.lastname: Opekarova
authors.forename: Petra
authors.initials: P
authors.lastname: Vesela
authors.forename: Iva
authors.initials: I
authors.lastname: Jancikova
authors.forename: Jakub
authors.initials: J
authors.lastname: Zahumensky
authors.forename: Jan
authors.initials: J
authors.lastname: Malinsky
mesh_terms: D002462:Cell Membrane / Q000378:metabolism*; D021962:Membrane Microdomains / Q000378:metabolism*; D008564:Membrane Potentials / Q000502:physiology; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism
chemical_list: D029701:Saccharomyces cerevisiae Proteins
keywords: gel microdomains; lipid order; phase separation; time-resolved fluorescence; transmembrane potential