title: A bacterial mutant library as a tool to study the attack of a defensin peptide.
abstract: Defensin attack! Here we report the screening of human defensin 5 against the Keio Collection of E. coli strains. The results of this screen further our understanding of how this important hostdefense peptide kills bacteria and how bacteria protect themselves against the attack from the human host.
authors.affiliation: Department of Chemistry, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139 (USA).
authors.forename: Simone
authors.initials: S
authors.lastname: Moser
authors.forename: Haritha R
authors.initials: HR
authors.lastname: Chileveru
authors.forename: Jill
authors.initials: J
authors.lastname: Tomaras
authors.forename: Elizabeth M
authors.initials: EM
authors.lastname: Nolan
mesh_terms: D000595:Amino Acid Sequence; D004926:Escherichia coli / Q000235:genetics / Q000276:immunology / Q000502:physiology*; D004927:Escherichia coli Infections / Q000276:immunology* / Q000382:microbiology; D015723:Gene Library; D054884:Host-Pathogen Interactions; D006801:Humans; D007113:Immunity, Innate; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009154:Mutation; D023084:alpha-Defensins / Q000737:chemistry / Q000276:immunology*
chemical_list: C500225:DEFA5 protein, human; D023084:alpha-Defensins
keywords: Keio Collection; human defensins; innate immunity; knockout mutants; lipopolysaccharide