title: Placental extracts induce the expression of antioxidant enzyme genes and suppress melanogenesis in B16 melanoma cells.
abstract: One of the activities of placental extracts (PEs) is skin-whitening effect, but the physiological and genetic mechanism for this effect has not yet been clarified. Here, we focus on PE as a regulator of antioxidant enzyme genes. Porcine PE was prepared, and its activity was investigated in B16 melanoma cells. PE treatment decreased the melanin content of UV-irradiated B16 cells in a dose-dependent manner. PE directly reduced the enzyme activity of tyrosinase in a cell-free assay. In addition, PE treatment increased the gene expression of cytosolic superoxide dismutase (SOD-1), extracellular SOD (SOD-3) and catalase but did not affect the expression of tyrosinase. Moreover, PE protected the B16 cells from H2O2-induced cell death. Taken together, our data suggest that PEs could play a role not only as a suppressor of melanin synthesis but also as a regulator of antioxidant genes and might protect the skin against oxidative stress.
authors.affiliation: a Department of Health Chemistry , Hoshi University , 2-4-41, Ebara, Shinagawa-Ku, Tokyo 142-8501 , Japan.
authors.forename: Masahiro
authors.initials: M
authors.lastname: Yamasaki
authors.affiliation: a Department of Health Chemistry , Hoshi University , 2-4-41, Ebara, Shinagawa-Ku, Tokyo 142-8501 , Japan.
authors.forename: Shinya
authors.initials: S
authors.lastname: Hasegawa
authors.affiliation: b Snowden Corporation , Chiyoda-Ku, Tokyo 101-0031 , Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Takahashi
authors.affiliation: a Department of Health Chemistry , Hoshi University , 2-4-41, Ebara, Shinagawa-Ku, Tokyo 142-8501 , Japan.
authors.forename: Yuka
authors.initials: Y
authors.lastname: Kobayashi
authors.affiliation: a Department of Health Chemistry , Hoshi University , 2-4-41, Ebara, Shinagawa-Ku, Tokyo 142-8501 , Japan.
authors.forename: Chihiro
authors.initials: C
authors.lastname: Sakai
authors.affiliation: a Department of Health Chemistry , Hoshi University , 2-4-41, Ebara, Shinagawa-Ku, Tokyo 142-8501 , Japan.
authors.forename: Yoko
authors.initials: Y
authors.lastname: Ashizawa
authors.affiliation: a Department of Health Chemistry , Hoshi University , 2-4-41, Ebara, Shinagawa-Ku, Tokyo 142-8501 , Japan.
authors.forename: Yuuki
authors.initials: Y
authors.lastname: Asai
authors.affiliation: a Department of Health Chemistry , Hoshi University , 2-4-41, Ebara, Shinagawa-Ku, Tokyo 142-8501 , Japan.
authors.forename: Mamiko
authors.initials: M
authors.lastname: Kanzaki
authors.affiliation: a Department of Health Chemistry , Hoshi University , 2-4-41, Ebara, Shinagawa-Ku, Tokyo 142-8501 , Japan.
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Fukui
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D002374:Catalase / Q000378:metabolism; D045744:Cell Line, Tumor; D008543:Melanins / Q000378:metabolism; D008546:Melanoma, Experimental / Q000378:metabolism*; D014442:Monophenol Monooxygenase / Q000037:antagonists & inhibitors / Q000378:metabolism; D010924:Placental Extracts / Q000494:pharmacology*; D013482:Superoxide Dismutase / Q000378:metabolism; D013552:Swine
chemical_list: D000975:Antioxidants; D008543:Melanins; D010924:Placental Extracts; D002374:Catalase; D014442:Monophenol Monooxygenase; D013482:Superoxide Dismutase
keywords: antioxidant enzyme; melanin synthesis; placenta extracts; superoxide dismutase