title: The novel surfactant protein SP-H enhances the phagocytosis efficiency of macrophage-like cell lines U937 and MH-S.
abstract: BACKGROUND
  Surfactant proteins (SP) secreted by alveolar type 2 cells, play an essential role in maintaining the air-liquid barrier of the lung and are also involved in the opsonisation and clearance of bacteria by phagocytes. We have recently described a novel surfactant protein, SP-H (SFTA3). Expression of SP-H was earlier demonstrated to be upregulated by LPS and negatively regulated by IL-1β and IL-23 in vitro. The influence of SP-H on phagocytosis was measured using a murine and a human phagocytic cell line and fluorescent latex beads.
  
  
  RESULTS
  SP-H markedly increases phagocytosis in vitro in the murine-derived alveolar macrophage cell lines MH-S and in human-derived differentiated U937 cells.
  
  
  CONCLUSIONS
  It can be assumed that SP-H is involved in regulating phagocytic activity of macrophages. SP-H is a new player in pulmonary host defence.
authors.forename: Ebru
authors.initials: E
authors.lastname: Diler
authors.forename: Martin
authors.initials: M
authors.lastname: Schicht
authors.forename: Andrea
authors.initials: A
authors.lastname: Rabung
authors.affiliation: Institute of Anatomy and Cell Biology, Saarland University, 66421 Homburg/Saar, Germany. thomas.tschernig@uks.eu.
authors.forename: Thomas
authors.initials: T
authors.lastname: Tschernig
authors.forename: Carola
authors.initials: C
authors.lastname: Meier
authors.forename: Felix
authors.initials: F
authors.lastname: Rausch
authors.forename: Fabian
authors.initials: F
authors.lastname: Garreis
authors.forename: Lars
authors.initials: L
authors.lastname: Bräuer
authors.forename: Friedrich
authors.initials: F
authors.lastname: Paulsen
mesh_terms: D000818:Animals; D002460:Cell Line; D004305:Dose-Response Relationship, Drug; D005453:Fluorescence; D006801:Humans; D008214:Lymphocytes / Q000166:cytology / Q000187:drug effects* / Q000276:immunology; D016676:Macrophages, Alveolar / Q000166:cytology / Q000187:drug effects* / Q000276:immunology; D051379:Mice; D008863:Microspheres; D009928:Organ Specificity; D010587:Phagocytosis; D037661:Pulmonary Surfactant-Associated Proteins / Q000494:pharmacology*; D013045:Species Specificity
chemical_list: D037661:Pulmonary Surfactant-Associated Proteins; C000597598:SFTA3 protein, human