title: Lipoxygenase inhibitory activity of Cuspidaria pulchra and isolated compounds.
abstract: This work evaluated the in vitro inhibitory activity of the crude ethanolic extract from the aerial parts of Cuspidaria pulchra (Cham.) L.G. Lohmann against 15-lipoxygenase (15-LOX). The bioassay-guided fractionation of the n-butanol fraction, which displayed the highest activity, led to the isolation of three compounds: caffeoylcalleryanin (1), verbascoside (2) and 6-hydroxyluteolin-7-O-β-glucoside (3). Assessment of the ability of the isolated compounds to inhibit 15-LOX revealed that compounds 1, 2 and 3 exerted strong 15-LOX inhibitory activity; IC50 values were 1.59, 1.76 and 2.35 μM respectively. The XTT assay showed that none of the isolated compounds seemed to be significantly toxic.
authors.affiliation: a Núcleo de Pesquisa em Ciências Exatas e Tecnológicas, Universidade de Franca , Av. Dr. Armando Salles de Oliveira, 201, 14404-600 Franca , Brazil.
authors.forename: Tavane A
authors.initials: TA
authors.lastname: Alvarenga
authors.forename: Camila S
authors.initials: CS
authors.lastname: Bertanha
authors.forename: Pollyanna F
authors.initials: PF
authors.lastname: de Oliveira
authors.forename: Denise C
authors.initials: DC
authors.lastname: Tavares
authors.forename: Valéria M M
authors.initials: VM
authors.lastname: Gimenez
authors.forename: Márcio L A
authors.initials: ML
authors.lastname: Silva
authors.forename: Wilson R
authors.initials: WR
authors.lastname: Cunha
authors.forename: Ana H
authors.initials: AH
authors.lastname: Januário
authors.forename: Patrícia M
authors.initials: PM
authors.lastname: Pauletti
mesh_terms: D029402:Bignoniaceae / Q000737:chemistry*; D002109:Caffeic Acids / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D005960:Glucosides / Q000737:chemistry / Q000302:isolation & purification; D016859:Lipoxygenase Inhibitors / Q000494:pharmacology*; D047311:Luteolin / Q000737:chemistry / Q000302:isolation & purification; D015394:Molecular Structure; D010636:Phenols / Q000737:chemistry / Q000302:isolation & purification; D035261:Plant Components, Aerial / Q000737:chemistry; D010936:Plant Extracts / Q000494:pharmacology*; D059808:Polyphenols
chemical_list: D002109:Caffeic Acids; D005960:Glucosides; D016859:Lipoxygenase Inhibitors; D047311:Luteolin; D010636:Phenols; D010936:Plant Extracts; C017406:6-hydroxyluteolin-7-O-glucoside; C000599314:caffeoylcalleryanin; C058956:acteoside; D059808:Polyphenols
keywords: 15-lipoxygenase inhibitory activity; Arrabidaea; cytotoxic activity