title: Mycelial fermentation characteristics and antiproliferative activity of Phellinus vaninii Ljup.
abstract: BACKGROUND
  The mycelial fermentation of higher fungi were investigated to posses various bioactivities.
  
  
  METHODS
  The mycelial growth and pellet morphology in a 5-L bioreactor were investigated. The mycelial broth containing biomass and extracellular products harvested from the fermentor was tested for antiproliferative activity of colon cancer LoVo cells using 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide assay.
  
  
  RESULTS
  The maximum mycelial concentration in a 5-L bioreactor was 12.5 g/L after 8 days cultivation. Further investigation in the mycelial pellets during the fermentation period revealed that the mean diameter of the pellet morphology was positively correlated with mycelial biomass (R 2 = 0.82, P < 0.05) and broth viscosity (R 2 = 0.90, P < 0.01), significantly. The ethyl acetate extract showed the most significant effects, increasing the inhibition rate up to 87.5% after 48 h at concentration of 1000 μg/mL.
  
  
  CONCLUSIONS
  The results demonstrated the feasibility of P. vaninii Ljup mycelial fermentation for large-scale production of bioactive and medicinal compounds.
authors.affiliation: Branch of Forest Protection, School of Forestry, Northeast Forestry University, Mudanjiang 157011, China ; Heilongjiang Forest By-product and Specialty Institute, Mudanjiang 157011, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Hu
authors.affiliation: College of Food and Biological Engineering, Zhengzhou University of Light Industry, Zhengzhou, Henan 450002, China.
authors.forename: Shuai
authors.initials: S
authors.lastname: Liu
authors.affiliation: Heilongjiang Forest By-product and Specialty Institute, Mudanjiang 157011, China.
authors.forename: Yuexin
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Heilongjiang Forestry Protection Institute, Harbin 150040, China.
authors.forename: Deng
authors.initials: D
authors.lastname: Xun
authors.affiliation: College of Food and Biological Engineering, Zhengzhou University of Light Industry, Zhengzhou, Henan 450002, China.
authors.forename: Chunping
authors.initials: C
authors.lastname: Xu
keywords: Antiproliferative activity; Phellinus vaninii Ljup; fermentation; morphology; mycelia