title: Monitoring mitophagy in mammalian cells.
abstract: It is essential for a cell to maintain a proper mitochondrial quality and quantity for normal cellular functions. Damaged or unwanted mitochondria can be selectively removed through mitophagy. Mitophagy research has attracted great attention from life sciences and biomedical fields, it is thus important for the community to properly measure mitophagy. Here, we will focus on the current techniques that have been used to monitor mitophagy in mammalian cells, including morphological and biochemical parameters for monitoring the occurrence of mitophagy.
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology, Tianjin Key Laboratory of Protein Science, College of Life Sciences, Nankai University, Tianjin, China.
authors.forename: Yushan
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology, Tianjin Key Laboratory of Protein Science, College of Life Sciences, Nankai University, Tianjin, China.
authors.forename: Guo
authors.initials: G
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology, Tianjin Key Laboratory of Protein Science, College of Life Sciences, Nankai University, Tianjin, China.
authors.forename: Linbo
authors.initials: L
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Biomembrane and Membrane Biotechnology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China.
authors.forename: Weiling
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Guangdong Key laboratory of Age-related Cardiac-cerebral Vascular Disease, Institute of Neurology, Guangdong Medical College, Zhanjiang, Guangdong Province, China.
authors.forename: Du
authors.initials: D
authors.lastname: Feng
authors.affiliation: State Key Laboratory of Biomembrane and Membrane Biotechnology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology, Tianjin Key Laboratory of Protein Science, College of Life Sciences, Nankai University, Tianjin, China; State Key Laboratory of Biomembrane and Membrane Biotechnology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China. Electronic address: chenq@ioz.ac.cn.
authors.forename: Quan
authors.initials: Q
authors.lastname: Chen
mesh_terms: D000818:Animals; D015153:Blotting, Western / Q000379:methods; D002518:Ceramides / Q000494:pharmacology; D005456:Fluorescent Dyes / Q000032:analysis / Q000378:metabolism; D006801:Humans; D008322:Mammals; D046529:Microscopy, Electron, Transmission / Q000379:methods; D008856:Microscopy, Fluorescence / Q000379:methods; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism; D063306:Mitophagy / Q000187:drug effects; D008967:Molecular Biology / Q000379:methods*; D015336:Molecular Probe Techniques
chemical_list: D002518:Ceramides; D005456:Fluorescent Dyes
keywords: Autophagy; Electron microscopy; LC3; Mitophagy; Western blot