title: Experimental approaches for measuring pKa's in RNA and DNA.
abstract: RNA and DNA carry out diverse functions in biology including catalysis, splicing, gene regulation, and storage of genetic information. Interest has grown in understanding how nucleic acids perform such sophisticated functions given their limited molecular repertoire. RNA can fold into diverse shapes that often perturb pKa values and allow it to ionize appreciably under biological conditions, thereby extending its molecular diversity. The goal of this chapter is to enable experimental measurement of pKa's in RNA and DNA. A number of experimental methods for measuring pKa values in RNA and DNA have been developed over the last 10 years, including RNA cleavage kinetics; UV-, fluorescence-, and NMR-detected pH titrations; and Raman crystallography. We begin with general considerations for choosing a pKa assay and then describe experimental conditions, advantages, and disadvantages for these assays. Potential pitfalls in measuring a pKa are provided including the presence of apparent pKa's due to a kinetic pKa or coupled acid- and alkali-promoted RNA unfolding, as well as degradation of RNA, precipitation of metal hydroxides and poor baselines. Use of multiple data fitting procedures and the study of appropriate mutants are described as ways to avoid some of these pitfalls. Application of these experimental methods to RNA and DNA will increase the number of available nucleic acid pKa values in the literature, which should deepen insight into biology and provide benchmarks for pKa calculations. Future directions for measuring pKa's in nucleic acids are discussed.
authors.affiliation: Department of Chemistry and Center for RNA Molecular Biology, The Pennsylvania State University, University Park, Pennsylvania, USA.
authors.forename: Pallavi
authors.initials: P
authors.lastname: Thaplyal
authors.affiliation: Department of Chemistry and Center for RNA Molecular Biology, The Pennsylvania State University, University Park, Pennsylvania, USA. Electronic address: pcb5@psu.edu.
authors.forename: Philip C
authors.initials: PC
authors.lastname: Bevilacqua
mesh_terms: D001483:Base Sequence; D003461:Crystallography / Q000379:methods; D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D007700:Kinetics; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods; D012313:RNA / Q000737:chemistry* / Q000378:metabolism; D059370:RNA Folding; D020871:RNA Stability; D016337:RNA, Catalytic / Q000737:chemistry / Q000378:metabolism; D013050:Spectrometry, Fluorescence / Q000379:methods; D013059:Spectrum Analysis, Raman / Q000379:methods
chemical_list: D016337:RNA, Catalytic; D012313:RNA; D004247:DNA
keywords: Absorbance; Fluorescence; NMR; RNA biology; Raman crystallography; Ribozyme; Spectroscopy; Theory; pK(a) methods