title: Identification of rhabdoviral sequences in oropharyngeal swabs from German and Danish bats.
abstract: BACKGROUND
  In the frame of active lyssavirus surveillance in bats, oropharyngeal swabs from German (N = 2297) and Danish (N = 134) insectivorous bats were investigated using a newly developed generic pan-lyssavirus real-time reverse transcriptase PCR (RT-qPCR).
  
  
  RESULTS
  In total, 15 RT-qPCR positive swabs were detected. Remarkably, sequencing of positive samples did not confirm the presence of bat associated lyssaviruses but revealed nine distinct novel rhabdovirus-related sequences.
  
  
  CONCLUSIONS
  Several novel rhabdovirus-related sequences were detected both in German and Danish insectivorous bats. The results also prove that the novel generic pan-lyssavirus RT-qPCR offers a very broad detection range that allows the collection of further valuable data concerning the broad and complex diversity within the family Rhabdoviridae.
authors.affiliation: Institute of Diagnostic Virology, Friedrich-Loeffler-Institut, Südufer 10, D-17493, Greifswald-Insel Riems, Germany. mlnfis@aol.com.
authors.forename: Melina
authors.initials: M
authors.lastname: Fischer
authors.affiliation: Institute of Molecular Virology and Cell Biology, Friedrich-Loeffler-Institut, Südufer 10, D-17493, Greifswald-Insel Riems, Germany. Conrad.Freuling@fli.bund.de.
authors.forename: Conrad M
authors.initials: CM
authors.lastname: Freuling
authors.affiliation: Institute of Molecular Virology and Cell Biology, Friedrich-Loeffler-Institut, Südufer 10, D-17493, Greifswald-Insel Riems, Germany. Thomas.Muller@fli.bund.de.
authors.forename: Thomas
authors.initials: T
authors.lastname: Müller
authors.affiliation: Institute of Molecular Virology and Cell Biology, Friedrich-Loeffler-Institut, Südufer 10, D-17493, Greifswald-Insel Riems, Germany. julianeschatz@hotmail.com.
authors.forename: Juliane
authors.initials: J
authors.lastname: Schatz
authors.affiliation: DTU Vet, Technical University of Denmark, Lindholm, DK-4771, Kalvehave, Denmark. tbrur@vet.dtu.dk.
authors.forename: Thomas Bruun
authors.initials: TB
authors.lastname: Rasmussen
authors.affiliation: DTU Vet, Technical University of Denmark, DK-1870, Frederiksberg C, Denmark. march@vet.dtu.dk.
authors.forename: Mariann
authors.initials: M
authors.lastname: Chriel
authors.affiliation: Institute of Novel and Emerging Infectious Diseases, Friedrich-Loeffler-Institut, Südufer 10, D-17493, Greifswald-Insel Riems, Germany. anne.balkema-buschmann@fli.bund.de.
authors.forename: Anne
authors.initials: A
authors.lastname: Balkema-Buschmann
authors.affiliation: Institute of Diagnostic Virology, Friedrich-Loeffler-Institut, Südufer 10, D-17493, Greifswald-Insel Riems, Germany. Martin.Beer@fli.bund.de.
authors.forename: Martin
authors.initials: M
authors.lastname: Beer
authors.affiliation: Institute of Diagnostic Virology, Friedrich-Loeffler-Institut, Südufer 10, D-17493, Greifswald-Insel Riems, Germany. Bernd.Hoffmann@fli.bund.de.
authors.forename: Bernd
authors.initials: B
authors.lastname: Hoffmann
mesh_terms: D000818:Animals; D002685:Chiroptera / Q000821:virology*; D003718:Denmark; D005858:Germany; D008969:Molecular Sequence Data; D009960:Oropharynx / Q000821:virology*; D012367:RNA, Viral / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012209:Rhabdoviridae / Q000302:isolation & purification*; D018353:Rhabdoviridae Infections / Q000662:veterinary* / Q000821:virology; D017422:Sequence Analysis, DNA
chemical_list: D012367:RNA, Viral