title: Ion channel probes for scanning ion conductance microscopy.
abstract: The sensitivity and selectivity of ion channels provide an appealing opportunity for sensor development. Here, we describe ion channel probes (ICPs), which consist of multiple ion channels reconstituted into lipid bilayers suspended across the opening of perflourinated glass micropipets. When incorporated with a scanning ion conductance microscope (SICM), ICPs displayed a distance-dependent current response that depended on the number of ion channels in the membrane. With distance-dependent current as feedback, probes were translated laterally, to demonstrate the possibility of imaging with ICPs. The ICP platform yields several potential advantages for SICM that will enable exciting opportunities for incorporation of chemical information into imaging and for high-resolution imaging.
authors.affiliation: Department of Chemistry, Indiana University , 800 E. Kirkwood Avenue, Bloomington, Indiana 47405, United States.
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhou
authors.forename: Leonard K
authors.initials: LK
authors.lastname: Bright
authors.forename: Wenqing
authors.initials: W
authors.lastname: Shi
authors.forename: Craig A
authors.initials: CA
authors.lastname: Aspinwall
authors.forename: Lane A
authors.initials: LA
authors.lastname: Baker
mesh_terms: D015374:Biosensing Techniques; D004553:Electric Conductivity; D005246:Feedback; D007473:Ion Channels / Q000737:chemistry / Q000378:metabolism*; D008051:Lipid Bilayers / Q000378:metabolism; D020527:Microscopy, Scanning Probe / Q000379:methods*; D008958:Models, Molecular; D011487:Protein Conformation
chemical_list: D007473:Ion Channels; D008051:Lipid Bilayers