title: Colchicine treatment reversibly blocks cytokinesis but not mitosis in Trypanosoma cruzi epimastigotes.
abstract: This work analyzes the effect of the alkaloid colchicine on the growth of Trypanosoma cruzi epimastigotes, using immunofluorescence microscopy and flow cytometry techniques. We found that colchicine reversibly inhibited cytokinesis but not synthesis or segregation of nuclear and kinetoplastid DNA, in a concentration-dependent manner. We showed that, once colchicine was removed from the growth medium, cytokinesis was restored but abnormal segregation of kinetoplasts and nuclei generated zoids and parasites with two nuclei and one kinetoplast, among other aberrant cells. After drug removal, we also observed a few anucleated cells carrying two kinetoplasts in a stage compatible with the end of cytokinesis. The anomalous subcellular localization of the kinetoplast and flagellum observed in treated parasites suggests that the effect of colchicine and its interaction with T. cruzi microtubules is cell cycle dependent. The crosstalk between nuclear and kinetoplastid mitosis and its incidence on flagellum growth and parasite cell division regulation are discussed.
authors.affiliation: Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. H.N. Torres", INGEBI-CONICET, Vuelta de Obligado 2490, C1428ADN, Buenos Aires, Argentina, mariana.potenza@ingebi.conicet.gov.ar.
authors.forename: Mariana
authors.initials: M
authors.lastname: Potenza
authors.forename: María Teresa
authors.initials: MT
authors.lastname: Tellez-Iñón
mesh_terms: D000981:Antiprotozoal Agents / Q000494:pharmacology*; D002453:Cell Cycle / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002467:Cell Nucleus / Q000235:genetics / Q000378:metabolism; D003078:Colchicine / Q000494:pharmacology*; D048749:Cytokinesis / Q000187:drug effects*; D008870:Microtubules / Q000378:metabolism; D008938:Mitosis / Q000187:drug effects*; D014349:Trypanosoma cruzi / Q000166:cytology / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism
chemical_list: D000981:Antiprotozoal Agents; D003078:Colchicine