title: Comparative transcriptomic analysis of the response to cold acclimation in Eucalyptus dunnii.
abstract: Eucalyptus dunnii is an important macrophanerophyte with high economic value. However, low temperature stress limits its productivity and distribution. To study the cold response mechanisms of E. dunnii, 5 cDNA libraries were constructed from mRNA extracted from leaves exposed to cold stress for varying lengths of time and were evaluated by RNA-Seq analysis. The assembly of the Illumina datasets was optimized using various assembly programs and parameters. The final optimized assembly generated 205,325 transcripts with an average length of 1,701 bp and N50 of 2,627 bp, representing 349.38 Mb of the E. dunnii transcriptome. Among these transcripts, 134,358 transcripts (65.4%) were annotated in the Nr database. According to the differential analysis results, most transcripts were up-regulated as the cold stress prolonging, suggesting that these transcripts may be involved in the response to cold stress. In addition, the cold-relevant GO categories, such as 'response to stress' and 'translational initiation', were the markedly enriched GO terms. The assembly of the E. dunnii gene index and the GO classification performed in this study will serve as useful genomic resources for the genetic improvement of E. dunnii and also provide insights into the molecular mechanisms of cold acclimation in E. dunnii.
authors.affiliation: Department of Ornamental Horticulture, China Agricultural University, Beijing 100193, China; College of Life Science & Forestry, Chongqing University of Art & Science, Yongchuan 402160, China.
authors.forename: Yiqing
authors.initials: Y
authors.lastname: Liu
authors.affiliation: College of Life Science & Forestry, Chongqing University of Art & Science, Yongchuan 402160, China.
authors.forename: Yusong
authors.initials: Y
authors.lastname: Jiang
authors.affiliation: College of Life Science & Forestry, Chongqing University of Art & Science, Yongchuan 402160, China.
authors.forename: Jianbin
authors.initials: J
authors.lastname: Lan
authors.affiliation: College of Life Science & Forestry, Chongqing University of Art & Science, Yongchuan 402160, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Zou
authors.affiliation: Department of Ornamental Horticulture, China Agricultural University, Beijing 100193, China.
authors.forename: Junping
authors.initials: J
authors.lastname: Gao
mesh_terms: D000064:Acclimatization; D003080:Cold Temperature; D005052:Eucalyptus / Q000235:genetics / Q000502:physiology*; D020869:Gene Expression Profiling / Q000379:methods*; D018506:Gene Expression Regulation, Plant; D015723:Gene Library; D018515:Plant Leaves / Q000235:genetics; D010940:Plant Proteins / Q000235:genetics*; D017423:Sequence Analysis, RNA / Q000379:methods*
chemical_list: D010940:Plant Proteins