title: MicroRNA-1 promotes apoptosis of hepatocarcinoma cells by targeting apoptosis inhibitor-5 (API-5).
abstract: Although microRNA-1 (miR-1) is a known liver cancer suppressor, the role of miR-1 in apoptosis of hepatoma cells has remained largely unknown. Our study shows that ectopic miR-1 overexpression induced apoptosis of liver hepatocellular carcinoma (HepG2) cells. Apoptosis inhibitor 5 (API-5) was found to be a potential regulator of miR-1 induced apoptosis, using a bioinformatics approach. Furthermore, an inverse relationship between miR-1 and API-5 expression was observed in human liver cancer tissues and adjacent normal liver tissues. Negative regulation of API-5 expression by miR-1 was demonstrated to promote apoptosis of HepG2 cells. Our study provides a novel regulatory mechanism of miR-1 in the apoptosis of hepatoma cells.
authors.affiliation: Department of Oncology, Chengdu Military General Hospital, Chengdu 610083, Sichuan Province, China.
authors.forename: Dong
authors.initials: D
authors.lastname: Li
authors.affiliation: Department of Oncology, Chengdu Military General Hospital, Chengdu 610083, Sichuan Province, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Oncology, Chengdu Military General Hospital, Chengdu 610083, Sichuan Province, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Li
authors.affiliation: Department of Oncology, Chengdu Military General Hospital, Chengdu 610083, Sichuan Province, China.
authors.forename: Jing-Jing
authors.initials: JJ
authors.lastname: Peng
authors.affiliation: Department of Scientific Research and Training, Chengdu Military General Hospital, Chengdu 610083, Sichuan Province, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Tan
authors.affiliation: Institute of Aging and Immunity, Chengdu Medical College, Chengdu 610083, Sichuan Province, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Zou
authors.affiliation: Department of Health Care, Chengdu Military General Hospital, Chengdu 610083, Sichuan Province, China.
authors.forename: Xiao-Feng
authors.initials: XF
authors.lastname: Song
authors.affiliation: Department of Oncology, Chengdu Military General Hospital, Chengdu 610083, Sichuan Province, China.
authors.forename: Min
authors.initials: M
authors.lastname: Du
authors.affiliation: Department of Neurology, Chengdu Military General Hospital, Chengdu 610083, Sichuan Province, China.
authors.forename: Zheng-Hui
authors.initials: ZH
authors.lastname: Yang
authors.affiliation: Department of Oncology, Chengdu Military General Hospital, Chengdu 610083, Sichuan Province, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Tan
authors.affiliation: Department of Oncology, Chengdu Military General Hospital, Chengdu 610083, Sichuan Province, China.
authors.forename: Jin-Jun
authors.initials: JJ
authors.lastname: Zhou
authors.affiliation: Department of Oncology, Chengdu Military General Hospital, Chengdu 610083, Sichuan Province, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Xu
authors.affiliation: Department of Oncology, Chengdu Military General Hospital, Chengdu 610083, Sichuan Province, China.
authors.forename: Zeng-Qiang
authors.initials: ZQ
authors.lastname: Fu
authors.affiliation: Department of Oncology, Chengdu Military General Hospital, Chengdu 610083, Sichuan Province, China.
authors.forename: Jian-Qiong
authors.initials: JQ
authors.lastname: Feng
authors.affiliation: Department of Oncology, Chengdu Military General Hospital, Chengdu 610083, Sichuan Province, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Cheng
authors.affiliation: Department of Oncology, Chengdu Military General Hospital, Chengdu 610083, Sichuan Province, China.
authors.forename: Tao
authors.initials: T
authors.lastname: chen
authors.affiliation: Department of Oncology, Chengdu Military General Hospital, Chengdu 610083, Sichuan Province, China.
authors.forename: Dong
authors.initials: D
authors.lastname: Wei
authors.affiliation: Department of Oncology, Chengdu Military General Hospital, Chengdu 610083, Sichuan Province, China.
authors.forename: Xiao-Mei
authors.initials: XM
authors.lastname: Su
authors.affiliation: Department of Oncology, Chengdu Military General Hospital, Chengdu 610083, Sichuan Province, China.
authors.forename: Huan-Yi
authors.initials: HY
authors.lastname: Liu
authors.affiliation: Department of Oncology, Chengdu Military General Hospital, Chengdu 610083, Sichuan Province, China.
authors.forename: Zhong-Chun
authors.initials: ZC
authors.lastname: Qi
authors.affiliation: Department of General Surgery, Chengdu Military General Hospital, Chengdu 610083, Sichuan Province, China.
authors.forename: Li-Jun
authors.initials: LJ
authors.lastname: Tang
authors.affiliation: Department of General Surgery, Chengdu Military General Hospital, Chengdu 610083, Sichuan Province, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Wang
authors.affiliation: Department of Medical Lab, Chengdu Military General Hospital, Chengdu 610083, Sichuan Province, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Guo
authors.affiliation: Department of Oncology, Chengdu Military General Hospital, Chengdu 610083, Sichuan Province, China. Electronic address: huyonghe@vip.126.com.
authors.forename: Yong-He
authors.initials: YH
authors.lastname: Hu
authors.affiliation: Department of Oncology, Chengdu Military General Hospital, Chengdu 610083, Sichuan Province, China. Electronic address: 13438078785@163.com.
authors.forename: Tao
authors.initials: T
authors.lastname: Zhang
mesh_terms: D017209:Apoptosis; D051017:Apoptosis Regulatory Proteins / Q000096:biosynthesis* / Q000235:genetics; D006528:Carcinoma, Hepatocellular / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics; D056945:Hep G2 Cells; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D035683:MicroRNAs / Q000096:biosynthesis* / Q000235:genetics; D009363:Neoplasm Proteins / Q000096:biosynthesis* / Q000235:genetics; D009687:Nuclear Proteins / Q000096:biosynthesis* / Q000235:genetics; D012334:RNA, Neoplasm / Q000096:biosynthesis* / Q000235:genetics
chemical_list: C108386:API5 protein, human; D051017:Apoptosis Regulatory Proteins; C544547:MIRN1 microRNA, human; D035683:MicroRNAs; D009363:Neoplasm Proteins; D009687:Nuclear Proteins; D012334:RNA, Neoplasm
keywords: Apoptosis; Apoptosis inhibitor 5; Hepatocellular carcinoma; Hepatoma cell; microRNA-1