title: Calcite as a bone substitute. Comparison with hydroxyapatite and tricalcium phosphate with regard to the osteoblastic activity.
abstract: Close to the bone mineral phase, the calcic bioceramics, such as hydroxyapatite (HA) and β-tricalcium phosphate (β-TCP), are commonly used as substitutes or filling materials in bone surgery. Besides, calcium carbonate (CaCO3) is also used for their excellent biocompatibility and bioactivity. However, the problem with the animal-origin aragonite demands the new technique to synthesize pure calcite capable of forming 3D bone implant. This study aims to manufacture and evaluate a highly-pure synthetic crystalline calcite with good cytocompatibility regarding to the osteoblasts, comparing to that of HA and β-TCP. After the manufacture of macroporous bioceramic scaffolds with the identical internal architecture, their cytocompatibility is studied through MC3T3-E1 osteoblasts with the tests of cell viability, proliferation, vitality, etc. The results confirmed that the studied process is able to form a macroporous material with a controlled internal architecture, and this synthesized calcite is non-cytotoxic and facilitate the cell proliferation. Indeed requiring further improvement, the studied calcite is definitely an interesting alternative not only to coralline aragonite but also to calcium phosphate ceramics, particularly in bone sites with the large bone remodelling.
authors.affiliation: Laboratoire Génie Civil et géo-Environnement (EA 4515, Université Lille Nord de France), Equipe Biomatériaux Artois (Université d'Artois), IUT/GMP, 1230, rue de l'Université, BP 819, 62408 Béthune cedex, France. Francine.monchau@univ-artois.fr
authors.forename: F
authors.initials: F
authors.lastname: Monchau
authors.affiliation: Laboratoire Génie Civil et géo-Environnement (EA 4515, Université Lille Nord de France), Equipe Biomatériaux Artois (Université d'Artois), IUT/GMP, 1230, rue de l'Université, BP 819, 62408 Béthune cedex, France.
authors.forename: Ph
authors.initials: P
authors.lastname: Hivart
authors.affiliation: Laboratoire Génie Civil et géo-Environnement (EA 4515, Université Lille Nord de France), Equipe Biomatériaux Artois (Université d'Artois), IUT/GMP, 1230, rue de l'Université, BP 819, 62408 Béthune cedex, France.
authors.forename: B
authors.initials: B
authors.lastname: Genestie
authors.affiliation: Laboratoire Médicaments et Biomatériaux à Libération Contrôlée (INSERM U 1008, Université Lille Nord de France), Groupe de Recherche sur les Biomatériaux (Université Lille-2), Faculté de Médecine, 1, place de Verdun, 59045 Lille cedex, France.
authors.forename: F
authors.initials: F
authors.lastname: Chai
authors.affiliation: Laboratoire des Matériaux Céramiques et Procédés Associés (EA 2443, Université Lille Nord de France), Université de Valenciennes et du Hainaut-Cambrésis, ZI du Champ de l'Abbesse, 59600 Maubeuge, France.
authors.forename: M
authors.initials: M
authors.lastname: Descamps
authors.affiliation: Laboratoire Médicaments et Biomatériaux à Libération Contrôlée (INSERM U 1008, Université Lille Nord de France), Groupe de Recherche sur les Biomatériaux (Université Lille-2), Faculté de Médecine, 1, place de Verdun, 59045 Lille cedex, France.
authors.forename: H F
authors.initials: HF
authors.lastname: Hildebrand
mesh_terms: D000818:Animals; D018786:Bone Substitutes / Q000494:pharmacology*; D002118:Calcium / Q000032:analysis; D002119:Calcium Carbonate / Q000494:pharmacology*; D002130:Calcium Phosphates / Q000494:pharmacology*; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002516:Ceramics / Q000494:pharmacology; D019245:Compressive Strength / Q000187:drug effects; D017886:Durapatite / Q000494:pharmacology*; D004847:Epithelial Cells / Q000166:cytology / Q000187:drug effects; D006801:Humans; D051379:Mice; D010006:Osteoblasts / Q000166:cytology* / Q000187:drug effects; D010758:Phosphorus / Q000032:analysis; D019904:Polymethyl Methacrylate / Q000494:pharmacology; D016062:Porosity; D011208:Powders; D013500:Surface Tension / Q000187:drug effects; D054457:Tissue Scaffolds / Q000737:chemistry; D014961:X-Ray Diffraction
chemical_list: D018786:Bone Substitutes; D002130:Calcium Phosphates; D011208:Powders; D010758:Phosphorus; D019904:Polymethyl Methacrylate; D017886:Durapatite; D002119:Calcium Carbonate; C018392:tricalcium phosphate; D002118:Calcium