title: Identification of the biosynthetic gene clusters for the lipopeptides fusaristatin A and W493 B in Fusarium graminearum and F. pseudograminearum.
abstract: The closely related species Fusarium graminearum and Fusarium pseudograminearum differ in that each contains a gene cluster with a polyketide synthase (PKS) and a nonribosomal peptide synthetase (NRPS) that is not present in the other species. To identify their products, we deleted PKS6 and NRPS7 in F. graminearum and NRPS32 in F. pseudograminearum. By comparing the secondary metabolite profiles of the strains we identified the resulting product in F. graminearum as fusaristatin A, and as W493 A and B in F. pseudograminearum. These lipopeptides have previously been isolated from unidentified Fusarium species. On the basis of genes in the putative gene clusters we propose a model for biosynthesis where the polyketide product is shuttled to the NPRS via a CoA ligase and a thioesterase in F. pseudograminearum. In F. graminearum the polyketide is proposed to be directly assimilated by the NRPS.
authors.affiliation: Department of Biotechnology, Chemistry and Environmental Engineering, Aalborg University , DK-9000 Aalborg, Denmark.
authors.forename: Jens Laurids
authors.initials: JL
authors.lastname: Sørensen
authors.forename: Teis Esben
authors.initials: TE
authors.lastname: Sondergaard
authors.forename: Lorenzo
authors.initials: L
authors.lastname: Covarelli
authors.forename: Patricia Romans
authors.initials: PR
authors.lastname: Fuertes
authors.forename: Frederik Teilfeldt
authors.initials: FT
authors.lastname: Hansen
authors.forename: Rasmus John Normand
authors.initials: RJ
authors.lastname: Frandsen
authors.forename: Wagma
authors.initials: W
authors.lastname: Saei
authors.forename: Mie Bech
authors.initials: MB
authors.lastname: Lukassen
authors.forename: Reinhard
authors.initials: R
authors.lastname: Wimmer
authors.forename: Kristian Fog
authors.initials: KF
authors.lastname: Nielsen
authors.forename: Donald Max
authors.initials: DM
authors.lastname: Gardiner
authors.forename: Henriette
authors.initials: H
authors.lastname: Giese
mesh_terms: D047630:Depsipeptides / Q000737:chemistry / Q000302:isolation & purification*; D005670:Fusarium / Q000235:genetics / Q000378:metabolism*; D055666:Lipopeptides / Q000737:chemistry / Q000302:isolation & purification*; D015394:Molecular Structure; D005810:Multigene Family; D010453:Peptide Synthases / Q000378:metabolism; D010456:Peptides, Cyclic / Q000737:chemistry / Q000302:isolation & purification*; D048630:Polyketide Synthases / Q000378:metabolism*
chemical_list: D047630:Depsipeptides; D055666:Lipopeptides; D010456:Peptides, Cyclic; C500255:W493 B; C522435:fusaristatin A; D048630:Polyketide Synthases; D010453:Peptide Synthases; C104385:non-ribosomal peptide synthase