title: Genetic diversity of chloroquine-resistant Plasmodium vivax parasites from the western Brazilian Amazon.
abstract: The molecular basis of Plasmodium vivax chloroquine (CQ) resistance is still unknown. Elucidating the molecular background of parasites that are sensitive or resistant to CQ will help to identify and monitor the spread of resistance. By genotyping a panel of molecular markers, we demonstrate a similar genetic variability between in vitro CQ-resistant and sensitive phenotypes of P. vivax parasites. However, our studies identified two loci (MS8 and MSP1-B10) that could be used to discriminate between both CQ-susceptible phenotypes among P. vivax isolates in vitro. These preliminary data suggest that microsatellites may be used to identify and to monitor the spread of P. vivax-resistance around the world.
authors.affiliation: Laboratório de Infectologia e Parasitologia Molecular, Hospital Universitário Clementino Fraga Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brasil.
authors.forename: Omaira Vera
authors.initials: OV
authors.lastname: Lizcano
authors.affiliation: Laboratório de Malária, Centro de Pesquisa René Rachou-Fiocruz, Belo Horizonte, MG, Brasil.
authors.forename: Sarah Stela
authors.initials: SS
authors.lastname: Resende
authors.affiliation: Fundação de Medicina Tropical Dr Heitor Vieira Dourado, Manaus, AM, Brasil.
authors.forename: Yonne F
authors.initials: YF
authors.lastname: Chehuan
authors.affiliation: Fundação de Medicina Tropical Dr Heitor Vieira Dourado, Manaus, AM, Brasil.
authors.forename: Marcus V G
authors.initials: MV
authors.lastname: Lacerda
authors.affiliation: Laboratório de Malária, Centro de Pesquisa René Rachou-Fiocruz, Belo Horizonte, MG, Brasil.
authors.forename: Cristiana F A
authors.initials: CF
authors.lastname: Brito
authors.affiliation: Laboratório de Infectologia e Parasitologia Molecular, Hospital Universitário Clementino Fraga Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brasil.
authors.forename: Mariano G
authors.initials: MG
authors.lastname: Zalis
mesh_terms: D001938:Brazil / Q000453:epidemiology; D002738:Chloroquine / Q000494:pharmacology*; D016054:DNA, Protozoan / Q000302:isolation & purification*; D004351:Drug Resistance / Q000235:genetics*; D019353:Endemic Diseases / Q000706:statistics & numerical data; D005819:Genetic Markers; D014644:Genetic Variation; D006801:Humans; D016780:Malaria, Vivax / Q000097:blood / Q000453:epidemiology; D021261:Parasitic Sensitivity Tests; D010641:Phenotype; D010966:Plasmodium vivax / Q000187:drug effects* / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011897:Random Allocation
chemical_list: D016054:DNA, Protozoan; D005819:Genetic Markers; D002738:Chloroquine