title: Citrus transformation using juvenile tissue explants.
abstract: The most frequently used method for production of citrus transgenic plants is via Agrobacterium-mediated transformation of tissues found on explants obtained from juvenile seedlings. Within the last decade and especially within the last 5-6 years, this robust method was employed to produce thousands of transgenic plants. With the newly applied screening methods that allow easier and faster detection of transgenic shoots, estimates of transformation rate for some cultivars have gone up making this approach even more attractive. Although adjustments have to be made regarding the (varietal) source of the starting material and Agrobacterium strain used in each experiment preformed, the major steps of this procedure have not changed significantly if at all. Transgenic citrus plants produced this way belong to cultivars of rootstocks, sweet oranges, grapefruits, mandarins, limes, and lemons.
authors.affiliation: Citrus Research and Education Center, University of Florida/IFAS, 700 Experiment Station Road, Room 103, Lake Alfred, FL, 33850, USA, orbovic@ufl.edu.
authors.forename: Vladimir
authors.initials: V
authors.lastname: Orbović
authors.forename: Jude W
authors.initials: JW
authors.lastname: Grosser
mesh_terms: D016960:Agrobacterium tumefaciens / Q000235:genetics / Q000254:growth & development; D002957:Citrus / Q000166:cytology / Q000235:genetics* / Q000254:growth & development*; D018920:Coculture Techniques; D017930:Genes, Reporter / Q000235:genetics; D005818:Genetic Engineering / Q000379:methods*; D018520:Plant Shoots / Q000254:growth & development; D030821:Plants, Genetically Modified; D016133:Polymerase Chain Reaction; D014170:Transformation, Genetic; D019076:Transgenes / Q000235:genetics