title: Genetic traits of Vibrio cholerae O1 Haitian isolates that are absent in contemporary strains from Kolkata, India.
abstract: The world's worst cholera epidemic in Haiti (2010) coerced to trace the origin and dissemination of the causative agent Vibrio cholerae O1 for proper management of cholera. Sequence analysis of the Haitian strain showed several variations in the genes encoding cholera toxin B subunit (ctxB); toxin-co-regulated pilus (tcpA), repeat in toxins (rtxA), quinolone resistance-determining region (QRDR) of gyrase A (gyrA), rstB of RS element along with the change in the number of repeat sequences at the promoter region of ctxAB. Our earlier studies showed that variant tcpA (tcpA CIRS) and ctxB (ctxB7) first appeared in Kolkata during 2003 and 2006, respectively. The present study revealed that a variant rtxA was first isolated in Kolkata during 2004 and probably formed the genetic background for the emergence of the ctxB7 allele as we were unable to detect a single strain with the combination of El Tor rtxA and ctxB7. The variant gyrA was first time detected in Kolkata during 1994. The Kolkata strains contained four heptad repeats (TTTTGAT) in their CT promoter regions whereas Haitian strains carried 5 heptad repeats. Haitian strains had 3 nucleotide deletions at the rstB gene, which is a unique feature of the classical biotype strains. But the Kolkata strains did not have such deletion mutations in the rstB. Our study demonstrated the existence of some Haitian genetic traits in Kolkata isolates along with the dissimilarities in genomic content with respect to rstB and ctxAB promoter region. Finally, we conclude that Haitian variant strain may be evolved due to sequential event in the Indian subcontinent strain with some cryptic modification in the genome.
authors.affiliation: Division of Bacteriology, National Institute of Cholera, Enteric Diseases, Kolkata, India.
authors.forename: Priyanka
authors.initials: P
authors.lastname: Ghosh
authors.affiliation: Division of Bacteriology, National Institute of Cholera, Enteric Diseases, Kolkata, India.
authors.forename: Arindam
authors.initials: A
authors.lastname: Naha
authors.affiliation: Division of Bacteriology, National Institute of Cholera, Enteric Diseases, Kolkata, India.
authors.forename: G P
authors.initials: GP
authors.lastname: Pazhani
authors.affiliation: Division of Bacteriology, National Institute of Cholera, Enteric Diseases, Kolkata, India.
authors.forename: T
authors.initials: T
authors.lastname: Ramamurthy
authors.affiliation: Division of Bacteriology, National Institute of Cholera, Enteric Diseases, Kolkata, India.
authors.forename: Asish K
authors.initials: AK
authors.lastname: Mukhopadhyay
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics*; D001427:Bacterial Toxins / Q000235:genetics; D001483:Base Sequence; D002771:Cholera / Q000453:epidemiology / Q000382:microbiology*; D002772:Cholera Toxin / Q000235:genetics; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D019143:Evolution, Molecular; D036781:Fimbriae Proteins / Q000235:genetics; D014644:Genetic Variation; D006205:Haiti / Q000453:epidemiology; D006801:Humans; D007194:India; D008969:Molecular Sequence Data; D009154:Mutation; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic / Q000235:genetics; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics; D017422:Sequence Analysis, DNA; D013045:Species Specificity; D041241:Vibrio cholerae O1 / Q000145:classification / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; D001427:Bacterial Toxins; D004269:DNA, Bacterial; C434464:RtxA protein, Vibrio cholerae; C085807:TcpA protein, Vibrio cholerae; D036781:Fimbriae Proteins; D002772:Cholera Toxin