title: Hepatic Differentiation from Human Ips Cells Using M15 Cells.
abstract: Here, we describe a procedure of human iPS cells differentiation into the definitive endoderm, further into albumin-expressing and albumin-secreting hepatocyte, using M15, a mesonephros- derived cell line. Approximately 90 % of human iPS cells differentiated into SOX17-positive definitive endoderm then approximately 50 % of cells became albumin-positive cells, and secreted ALB protein. This M15 feeder system for endoderm and hepatic differentiation is a simple and efficient method, and useful for elucidating molecular mechanisms for hepatic fate decision, and could represent an attractive approach for a surrogate cell source for pharmaceutical studies.
authors.affiliation: Department of Stem Cell Biology, Institute of Molecular Embryology and Genetics (IMEG), Kumamoto University, Honjo 2-2-1, Chuo-ku, 860-0811, Kumamoto, Japan.|Program for Leading Graduate Schools "HIGO (Health life science; Interdisciplinary and Glocal Oriented) Program", Kumamoto University, Honjo 2-2-1, Chuo-ku, 860-0811, Kumamoto, Japan.
authors.forename: Kahoko
authors.initials: K
authors.lastname: Umeda
authors.affiliation: Department of Stem Cell Biology, Institute of Molecular Embryology and Genetics (IMEG), Kumamoto University, Honjo 2-2-1, Chuo-ku, 860-0811, Kumamoto, Japan.
authors.forename: Nobuaki
authors.initials: N
authors.lastname: Shiraki
authors.affiliation: Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama, Kanagawa, Japan. skume@kumamoto-u.ac.jp.
authors.forename: Shoen
authors.initials: S
authors.lastname: Kume
mesh_terms: D018929:Cell Culture Techniques / Q000379:methods*; D002460:Cell Line; D019070:Cell Lineage; D002478:Cells, Cultured; D065150:Cellular Reprogramming; D000067470:Cellular Reprogramming Techniques / Q000379:methods*; D003470:Culture Media / Q000494:pharmacology; D004707:Endoderm / Q000166:cytology*; D022781:Hepatocytes / Q000166:cytology*; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology*; D016685:Mitomycin / Q000494:pharmacology; D055760:SOXF Transcription Factors / Q000032:analysis; D012709:Serum Albumin / Q000096:biosynthesis
chemical_list: D003470:Culture Media; C109188:SOX17 protein, human; D055760:SOXF Transcription Factors; D012709:Serum Albumin; D016685:Mitomycin
keywords: Endoderm differentiation; Feeder cells; Hepatic differentiation; M15 cells