title: Evaluation of photodynamic therapy efficiency using an in vitro three-dimensional microfluidic breast cancer tissue model.
abstract: In recognition of the limitations of monolayer cell cultures and resource-intensive animal studies, a microfluidic culture system was developed for creation of physiologically relevant three-dimensional (3D) tissues. In this study, an in vitro 3D breast cancer tissue model was established in a microfluidic system with human breast cancer cells (MCF-7) and primary adipose-derived stromal cells (ASCs). It was evaluated for utility in determining the efficiency of photodynamic therapy (PDT) with therapeutic agents (i.e. photosensitizer and gold nanoparticles) under various irradiation conditions. We demonstrated, for the first time, the potential use of a microfluidic-based in vitro 3D breast cancer model for effective evaluation of PDT, with the capability of controlling 3D microenvironments for breast cancer tissue formation, real-time monitoring of tissue progression, implementing a circulation-like dynamic medium flow and drug supplements, and investigating the relation between light penetration and tissue depth in PDT.
authors.affiliation: Department of Chemistry, Chemical Biology and Biomedical Engineering, Stevens Institute of Technology, Hoboken, NJ 07030, USA. hongjun.wang@stevens.edu.
authors.forename: Yamin
authors.initials: Y
authors.lastname: Yang
authors.forename: Xiaochuan
authors.initials: X
authors.lastname: Yang
authors.forename: Jin
authors.initials: J
authors.lastname: Zou
authors.forename: Chao
authors.initials: C
authors.lastname: Jia
authors.forename: Yue
authors.initials: Y
authors.lastname: Hu
authors.forename: Henry
authors.initials: H
authors.lastname: Du
authors.forename: Hongjun
authors.initials: H
authors.lastname: Wang
mesh_terms: D001943:Breast Neoplasms / Q000378:metabolism / Q000473:pathology* / Q000628:therapy*; D002470:Cell Survival; D002478:Cells, Cultured; D004867:Equipment Design; D005260:Female; D006801:Humans; D061986:MCF-7 Cells; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation; D008954:Models, Biological; D010778:Photochemotherapy; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D017382:Reactive Oxygen Species