title: Modulation of the faecal microbiome of healthy adult dogs by inclusion of potato fibre in the diet.
abstract: Inclusion of fermentable fibres in the diet can have an impact on the hindgut microbiome and provide numerous health benefits to the host. Potato fibre (PF), a co-product of potato starch isolation, has a favourable chemical composition of pectins, resistant and digestible starch, cellulose, and hemicelluloses. The objective of the present study was to evaluate the effect of increasing dietary PF concentrations on the faecal microbiome of healthy adult dogs. Fresh faecal samples were collected from ten female dogs with hound bloodlines (6·13 (SEM 0·17) years; 22·0 (SEM 2·1) kg) fed five test diets containing graded concentrations of PF (0, 1·5, 3, 4·5 or 6% as-fed; Roquette Frères) in a replicated 5 × 5 Latin square design. Extraction of DNA was followed by amplification of the V4-V6 variable region of the 16S rRNA gene using barcoded primers. Sequences were classified into taxonomic levels using Basic Local Alignment Search Tool (BLASTn) against a curated GreenGenes database. Inclusion of PF increased (P< 0·05) the faecal proportions of Firmicutes, while those of Fusobacteria decreased (P< 0·05). Similar shifts were observed at the genus level and were confirmed by quantitative PCR (qPCR) analysis. With increasing concentrations of PF, faecal proportions of Faecalibacterium increased (P< 0·05). Post hoc Pearson's correlation analysis showed positive (P< 0·05) correlations with Bifidobacterium spp. and butyrate production and Lactobacillus spp. concentrations. Overall, increases in the proportion of Faecalibacterium (not Lactobacillus/Bifidobacterium, as confirmed by qPCR analysis) and faecal SCFA concentrations with increasing dietary PF concentrations suggest that PF is a possible prebiotic fibre.
authors.affiliation: Department of Animal Sciences,University of Illinois,1207 West Gregory Drive,Urbana,IL61801,USA.
authors.forename: Matthew R
authors.initials: MR
authors.lastname: Panasevich
authors.affiliation: Department of Animal Sciences,University of Illinois,1207 West Gregory Drive,Urbana,IL61801,USA.
authors.forename: Katherine R
authors.initials: KR
authors.lastname: Kerr
authors.affiliation: Department of Animal Sciences,University of Illinois,1207 West Gregory Drive,Urbana,IL61801,USA.
authors.forename: Ryan N
authors.initials: RN
authors.lastname: Dilger
authors.affiliation: Department of Animal Sciences,University of Illinois,1207 West Gregory Drive,Urbana,IL61801,USA.
authors.forename: George C
authors.initials: GC
authors.lastname: Fahey
authors.affiliation: Roquette Frères, Biology and Nutrition Department, Rue de la Haute Loge,Lestrem62080,France.
authors.forename: Laetitia
authors.initials: L
authors.lastname: Guérin-Deremaux
authors.affiliation: Roquette America, Inc.,2211 Innovation Drive,Geneva,IL60134,USA.
authors.forename: Gary L
authors.initials: GL
authors.lastname: Lynch
authors.affiliation: Roquette Frères, Biology and Nutrition Department, Rue de la Haute Loge,Lestrem62080,France.
authors.forename: Daniel
authors.initials: D
authors.lastname: Wils
authors.affiliation: Gastrointestinal Laboratory,Texas A&M University,College Station,TX77843,USA.
authors.forename: Jan S
authors.initials: JS
authors.lastname: Suchodolski
authors.affiliation: Gastrointestinal Laboratory,Texas A&M University,College Station,TX77843,USA.
authors.forename: Jörg M
authors.initials: JM
authors.lastname: Steer
authors.affiliation: MR DNA Molecular Research LP,503 Clovis Road,Shallowater,TX79363,USA.
authors.forename: Scot E
authors.initials: SE
authors.lastname: Dowd
authors.affiliation: Department of Animal Sciences,University of Illinois,1207 West Gregory Drive,Urbana,IL61801,USA.
authors.forename: Kelly S
authors.initials: KS
authors.lastname: Swanson
mesh_terms: D000818:Animals; D001644:Bifidobacterium / Q000302:isolation & purification; D000068016:Eubacteriales / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D004032:Diet / Q000662:veterinary*; D004043:Dietary Fiber / Q000008:administration & dosage*; D004285:Dogs / Q000382:microbiology*; D005243:Feces / Q000737:chemistry / Q000382:microbiology*; D005260:Female; D005285:Fermentation; D000068536:Bacillota / Q000302:isolation & purification; D045182:Fusobacteria / Q000302:isolation & purification; D000069196:Gastrointestinal Microbiome; D041981:Gastrointestinal Tract / Q000382:microbiology; D056692:Prebiotics / Q000008:administration & dosage; D012336:RNA, Ribosomal, 16S / Q000235:genetics / Q000302:isolation & purification; D011198:Solanum tuberosum / Q000737:chemistry*
chemical_list: D004269:DNA, Bacterial; D004043:Dietary Fiber; D056692:Prebiotics; D012336:RNA, Ribosomal, 16S
keywords: Dogs; Faecal microbiome; Potato fibre