title: Synergistic actions of olomoucine and bone morphogenetic protein-4 in axonal repair after acute spinal cord contusion.
abstract: To determine whether olomoucine acts synergistically with bone morphogenetic protein-4 in the treatment of spinal cord injury, we established a rat model of acute spinal cord contusion by impacting the spinal cord at the T8 vertebra. We injected a suspension of astrocytes derived from glial-restricted precursor cells exposed to bone morphogenetic protein-4 (GDAs(BMP)) into the spinal cord around the site of the injury, and/or olomoucine intraperitoneally. Olomoucine effectively inhibited astrocyte proliferation and the formation of scar tissue at the injury site, but did not prevent proliferation of GDAs(BMP) or inhibit their effects in reducing the spinal cord lesion cavity. Furthermore, while GDAs(BMP) and olomoucine independently resulted in small improvements in locomotor function in injured rats, combined administration of both treatments had a significantly greater effect on the restoration of motor function. These data indicate that the combined use of olomoucine and GDAs(BMP) creates a better environment for nerve regeneration than the use of either treatment alone, and contributes to spinal cord repair after injury.
authors.affiliation: Capital Medical University School of Rehabilitation Medicine, Beijing, China ; Department of Spinal and Neural Function Reconstruction, China Rehabilitation Research Center, Beijing, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Chen
authors.affiliation: Capital Medical University School of Rehabilitation Medicine, Beijing, China ; Department of Spinal and Neural Function Reconstruction, China Rehabilitation Research Center, Beijing, China ; Center of Neural Injury and Repair, Beijing Institute for Brain Disorders, Beijing, China.
authors.forename: Jianjun
authors.initials: J
authors.lastname: Li
authors.affiliation: Rehabilitation Center, Beijing Xiaotangshan Rehabilitation Hospital, Beijing, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Wu
authors.affiliation: Capital Medical University School of Rehabilitation Medicine, Beijing, China ; Department of Spinal and Neural Function Reconstruction, China Rehabilitation Research Center, Beijing, China ; Center of Neural Injury and Repair, Beijing Institute for Brain Disorders, Beijing, China.
authors.forename: Mingliang
authors.initials: M
authors.lastname: Yang
authors.affiliation: Capital Medical University School of Rehabilitation Medicine, Beijing, China ; Department of Spinal and Neural Function Reconstruction, China Rehabilitation Research Center, Beijing, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Gao
authors.affiliation: Department of General Surgery, China Rehabilitation Research Center, Beijing, China.
authors.forename: Li
authors.initials: L
authors.lastname: Yuan
keywords: axonal regeneration; cavity; glial scar; glial-restricted precursor-derived astrocytes; nerve regeneration; neural regeneration; olomoucine; spinal cord injury