title: [A method for PCR product cloning based on exonuclease III].
abstract: Gene cloning is one of the most important and widely used technologies in molecular biology research. Generally, DNA fragment is cut with restriction enzyme, and then the product is ligated to a linearized vector with complementary sticky end or blunt end by DNA-ligase. This traditional DNA cloning method requires compatible enzyme recognition sites existing in both PCR fragment and targeted vector. Several ligase-free methods have been established to avoid the using of restriction enzyme. However, those methods are time-consuming, labor-intensive and expensive. To overcome these shortcomings, we developed an Exonuclease III based DNA cloning method that takes only 30 minutes with high cloning efficiency and significant economic advantage. Therefore, this method is suitable for large-scale gene cloning.
authors.forename: Yanyan
authors.initials: Y
authors.lastname: Wang
authors.forename: Chunyu
authors.initials: C
authors.lastname: Zhang
authors.forename: Xingchun
authors.initials: X
authors.lastname: Wang
authors.forename: Bin
authors.initials: B
authors.lastname: Liu
mesh_terms: D003001:Cloning, Molecular / Q000379:methods*; D004247:DNA / Q000737:chemistry; D004262:DNA Restriction Enzymes; D005090:Exodeoxyribonucleases / Q000737:chemistry*; D005822:Genetic Vectors; D016133:Polymerase Chain Reaction
chemical_list: D004247:DNA; D005090:Exodeoxyribonucleases; C020133:exodeoxyribonuclease III; D004262:DNA Restriction Enzymes