title: Cynomolgus Monkey Induced Pluripotent Stem Cells Generated By Using Allogeneic Genes.
abstract: Induced pluripotent stem (iPS) cells that are potentially similar to embryonic stem (ES) cells can be artificially established by introduction into somatic cells of the transgenes POU5F1 (also known as Oct3/4), SOX2, KLF4, and c-MYC. In cynomolgus monkeys (Macaca fascicularis), iPS cells generated by using these four allogeneic transgenes should be an important resource for various types of biomedical research because the use of xenogeneic transgenes may cause complications. To establish such iPS cells, cynomolgus monkey somatic cells were infected with amphotropic retroviral vectors, which were derived from Plat-A cells, containing cDNA for the cynomolgus monkey genes POU5F1, SOX2, KLF4, and c-MYC. As a result, iPS cells could be established from somatic cells from fetal liver and newborn skin of cynomolgus monkeys, similarly to the case for mouse and human somatic cells.
authors.affiliation: Tsukuba Primate Research Center (TPRC), National Institute of Biomedical Innovation (NIBIO), 1-1 Hachimandai, Tsukuba, Ibaraki, 305-0843, Japan. shimo@nibio.go.jp.
authors.forename: Nobuhiro
authors.initials: N
authors.lastname: Shimozawa
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques; D002478:Cells, Cultured; D065150:Cellular Reprogramming; D000067470:Cellular Reprogramming Techniques / Q000379:methods; D015925:Cryopreservation; D018076:DNA, Complementary / Q000235:genetics; D005347:Fibroblasts / Q000166:cytology; D016259:Genes, myc; D005822:Genetic Vectors / Q000235:genetics; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology*; D000090062:Kruppel-Like Factor 4; D051741:Kruppel-Like Transcription Factors / Q000235:genetics / Q000502:physiology; D008099:Liver / Q000166:cytology / Q000196:embryology; D008252:Macaca fascicularis; D051379:Mice; D050814:Octamer Transcription Factor-3 / Q000235:genetics / Q000502:physiology; D020598:Proto-Oncogene Proteins c-myb / Q000502:physiology; D012190:Retroviridae / Q000235:genetics; D055748:SOXB1 Transcription Factors / Q000235:genetics / Q000502:physiology; D012867:Skin / Q000166:cytology / Q000196:embryology; D013045:Species Specificity; D019076:Transgenes; D058989:Vitrification
chemical_list: D018076:DNA, Complementary; C000715467:KLF4 protein, human; C000715468:Klf4 protein, mouse; D000090062:Kruppel-Like Factor 4; D051741:Kruppel-Like Transcription Factors; D050814:Octamer Transcription Factor-3; D020598:Proto-Oncogene Proteins c-myb; D055748:SOXB1 Transcription Factors
keywords: Amphotropic retroviral vector; Cell culture; Cynomolgus monkey; Cynomolgus monkey gene; Induced pluripotent stem cell; Medical research; Nonhuman primate; Plat-A cell