title: Ratiometric fluorescence detection of cysteine and homocysteine with a BODIPY dye by mimicking the native chemical ligation.
abstract: The selective detection of cysteine and homocysteine over glutathione and other amino acids was demonstrated with an 8-MeS-BODIPY probe by mimicking the native chemical ligation approach, which allowed the selective and ratiometric fluorescence sensing of cysteine over other biothiols at physiologically relevant concentrations and in different organs of a zebrafish.
authors.affiliation: Department of Chemistry and Center for Electro-Photo Behaviors in Advanced Molecular Systems, Pohang University of Science and Technology (POSTECH), 77 Cheongam-Ro, Nam-Gu, Pohang, 790-784, Republic of Korea. ahn@postech.ac.kr.
authors.forename: Dong Hee
authors.initials: DH
authors.lastname: Ma
authors.forename: Dokyoung
authors.initials: D
authors.lastname: Kim
authors.forename: Eunseok
authors.initials: E
authors.lastname: Seo
authors.forename: Sang-Joon
authors.initials: SJ
authors.lastname: Lee
authors.forename: Kyo Han
authors.initials: KH
authors.lastname: Ahn
mesh_terms: D000818:Animals; D001896:Boron Compounds / Q000737:chemistry*; D003545:Cysteine / Q000032:analysis*; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000737:chemistry*; D005978:Glutathione / Q000737:chemistry; D006710:Homocysteine / Q000032:analysis*; D018613:Microscopy, Confocal; D013438:Sulfhydryl Compounds / Q000032:analysis*; D015027:Zebrafish / Q000033:anatomy & histology
chemical_list: C095489:4,4-difluoro-4-bora-3a,4a-diaza-s-indacene; D001896:Boron Compounds; D005456:Fluorescent Dyes; D013438:Sulfhydryl Compounds; D006710:Homocysteine; D005978:Glutathione; D003545:Cysteine