title: Preparation of caffeic acid phenethyl ester-incorporated nanoparticles and their biological activity.
abstract: The aim of this study is to fabricate caffeic acid phenethyl ester (CAPE)-incorporated nanoparticles using methoxy poly(ethylene glycol)-b-poly(ε-caprolactone) (CE) copolymer and to study their antitumor activity against pulmonary metastasis model of CT26 colon carcinoma cells. CAPE-incorporated nanoparticles showed spherical shapes having small diameters less than 300 nm and CAPE was continuously released from CE nanoparticles over 4 days. CAPE-incorporated polymeric micelles properly inhibited proliferation and induced apoptosis of CT26 cells as well as CAPE itself. Furthermore, they showed similar anti-invasive and antimigrative effect against CT26 cells at in vitro compared with CAPE itself, indicating that CAPE-incorporated nanoparticles have at least equivalent anticarcinogenic activity against CT26 cells compared with CAPE itself. At pulmonary metastasis model of CT26 cells using nude mouse, CAPE-incorporated nanoparticles have superior antimetastatic efficacy against, that is, control treatment with pulmonary metastasis model showed significant increase of lung weight because of the metastasis of tumor cells, whereas CAPE or CAPE-incorporated nanoparticles properly inhibited metastasis of tumor cells. We suggest CAPE-incorporated nanoparticles as a promising candidate for antimetastatic chemotherapeutic agent. © 2014 Wiley Periodicals, Inc. and the American Pharmacists Association J Pharm Sci 104:144-154, 2015.
authors.affiliation: Department of Medical Science, Pusan National University School of Medicine, Yangsan, Gyeongnam, 626-770, Korea.
authors.forename: Hyo-Young
authors.initials: HY
authors.lastname: Lee
authors.forename: Young-Il
authors.initials: YI
authors.lastname: Jeong
authors.forename: Eun Jin
authors.initials: EJ
authors.lastname: Kim
authors.forename: Kyung Dong
authors.initials: KD
authors.lastname: Lee
authors.forename: Seon-Hee
authors.initials: SH
authors.lastname: Choi
authors.forename: Yun Jin
authors.initials: YJ
authors.lastname: Kim
authors.forename: Da Hye
authors.initials: DH
authors.lastname: Kim
authors.forename: Ki-Choon
authors.initials: KC
authors.lastname: Choi
mesh_terms: D000818:Animals; D016588:Anticarcinogenic Agents / Q000008:administration & dosage* / Q000009:adverse effects / Q000494:pharmacology / Q000627:therapeutic use; D000970:Antineoplastic Agents / Q000008:administration & dosage* / Q000009:adverse effects / Q000494:pharmacology / Q000627:therapeutic use; D017209:Apoptosis / Q000187:drug effects; D002109:Caffeic Acids / Q000008:administration & dosage* / Q000009:adverse effects / Q000494:pharmacology / Q000627:therapeutic use; D002277:Carcinoma / Q000188:drug therapy* / Q000473:pathology / Q000556:secondary; D002461:Cell Line, Transformed; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects; D015179:Colorectal Neoplasms / Q000473:pathology; D004337:Drug Carriers / Q000008:administration & dosage* / Q000009:adverse effects / Q000494:pharmacology / Q000627:therapeutic use; D004339:Drug Compounding; D008168:Lung / Q000187:drug effects / Q000473:pathology; D008175:Lung Neoplasms / Q000188:drug therapy* / Q000473:pathology / Q000556:secondary; D008264:Macrophages / Q000187:drug effects / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008823:Micelles; D053758:Nanoparticles / Q000009:adverse effects / Q000737:chemistry*; D009368:Neoplasm Transplantation; D010626:Phenylethyl Alcohol / Q000008:administration & dosage / Q000009:adverse effects / Q000031:analogs & derivatives* / Q000494:pharmacology / Q000627:therapeutic use; D012995:Solubility; D047368:Tumor Burden / Q000187:drug effects
chemical_list: D016588:Anticarcinogenic Agents; D000970:Antineoplastic Agents; D002109:Caffeic Acids; D004337:Drug Carriers; D008823:Micelles; C055494:caffeic acid phenethyl ester; D010626:Phenylethyl Alcohol
keywords: caffeic acid phenethyl ester; cancer chemotherapy; invasion; metastasis; nanoparticles; natural products; polymeric drug carrier