title: Biopsy of different oral soft tissues lesions by KTP and diode laser: histological evaluation.
abstract: BACKGROUND
  Oral biopsy aims to obtain clear and safe diagnosis; it can be performed by scalpel or laser. The controversy in this latter application is the thermal alteration due to tissue heating. The aim of this study is the histological evaluation of margins of "in vivo" biopsies collected by diode and KTP lasers.
  
  
  METHODS
  17 oral benign lesions biopsies were made by diode 808 nm (SOL, DenMatItalia, Italy) and KTP 532 nm (SmartLite, DEKA, Italy). Samples were observed at OM LEICA DM 2000; margin alterations were evaluated through Leica Application Suite 3.4.
  
  
  RESULTS
  Epithelial and connective damages were assessed for each pathology with an average of 0.245 mm and a standard deviation of ± 0.162 mm in mucoceles, 0.382 mm ± 0.149 mm in fibromas, 0.336 mm ± 0.106 mm in hyperkeratosis, 0.473 mm ± 0.105 mm in squamous hyperplasia, 0.182 mm in giant cell granuloma, and 0.149 mm in melanotic macula.
  
  
  CONCLUSIONS
  The histologic aspect of lesions influenced the response to laser, whereas the greater inflammation and cellularity were linked with the higher thermal signs. Many artifacts were also associated to histologic procedures.
  
  
  CONCLUSIONS
  Both tested lasers permitted sure histologic diagnosis. However, it is suggested to enlarge biopsies of about 0.5 mm, to avoid thermal alterations, especially in inflammatory lesions like oral lichen planus.
authors.affiliation: Department of Oral and Maxillofacial Sciences, "Sapienza" University of Rome, Via Caserta 6, 00161 Rome, Italy.
authors.forename: Umberto
authors.initials: U
authors.lastname: Romeo
authors.affiliation: Department of Oral and Maxillofacial Sciences, "Sapienza" University of Rome, Via Caserta 6, 00161 Rome, Italy.
authors.forename: Claudia
authors.initials: C
authors.lastname: Russo
authors.affiliation: Department of Oral and Maxillofacial Sciences, "Sapienza" University of Rome, Via Caserta 6, 00161 Rome, Italy.
authors.forename: Gaspare
authors.identifier: 0000-0001-8509-2255
authors.initials: G
authors.lastname: Palaia
authors.affiliation: Department of Oral and Maxillofacial Sciences, "Sapienza" University of Rome, Via Caserta 6, 00161 Rome, Italy.
authors.forename: Rossella
authors.initials: R
authors.lastname: Lo Giudice
authors.affiliation: Department of Oral and Maxillofacial Sciences, "Sapienza" University of Rome, Via Caserta 6, 00161 Rome, Italy.
authors.forename: Alessandro
authors.identifier: 0000-0002-9278-8872
authors.initials: A
authors.lastname: Del Vecchio
authors.affiliation: Department of Cytology and Cellular Diagnostics, Regina Elena Institute, Via Elio Chianesi 53, 00144 Rome, Italy.
authors.forename: Paolo
authors.initials: P
authors.lastname: Visca
authors.affiliation: Department of Oral and Maxillofacial Sciences, "Sapienza" University of Rome, Via Caserta 6, 00161 Rome, Italy.
authors.forename: Guido
authors.initials: G
authors.lastname: Migliau
authors.affiliation: Department of Oral and Maxillofacial Sciences, "Sapienza" University of Rome, Via Caserta 6, 00161 Rome, Italy.
authors.forename: Alberto
authors.identifier: 0000-0002-8475-463X
authors.initials: A
authors.lastname: De Biase
mesh_terms: D001706:Biopsy / Q000379:methods; D005260:Female; D005350:Fibroma / Q000175:diagnosis* / Q000473:pathology; D006101:Granuloma, Giant Cell / Q000175:diagnosis* / Q000473:pathology; D006358:Hot Temperature; D006801:Humans; D017488:Hyperkeratosis, Epidermolytic / Q000175:diagnosis* / Q000473:pathology; D054023:Lasers, Semiconductor / Q000009:adverse effects; D053844:Lasers, Solid-State / Q000009:adverse effects; D017676:Lichen Planus, Oral / Q000175:diagnosis* / Q000473:pathology; D008297:Male; D009055:Mouth / Q000473:pathology; D009078:Mucocele / Q000175:diagnosis* / Q000473:pathology