title: Pharmacokinetic of pseudoephedrine in rat serum with luminol-pepsin chemiluminescence system by flow injection analysis.
abstract: Pepsin (Pep) accelerated the electron transferring rate of excited 3-aminophathlate and enhanced luminol-dissolved oxygen chemiluminescence (CL) intensity, and the flow injection (FI) luminol-Pep CL system was first developed. It was found that the CL intensity of luminol-Pep reaction could be remarkably inhibited by pseudoephedrine (PE); the decrement of CL intensity was linear to the logarithm of PE concentration in the range of 0.1∼100.0 nmol L(-1) with a detection limit of 0.03 nmol mL(-1) (3σ). At a flow rate of 2.0 mL min(-1), the complete process including washing and sampling was performed within 40 s, offering a sample throughput of 90 h(-1). This proposed method was successfully applied to determining PE in rat serum for 18 h after intragastric administration with the elimination ratio of 42.34 % and recoveries from 90.3 to 110.6 %. The pharmacokinetic results showed that PE could be rapidly absorbed into serum with peak concentration (C max) of 1.45 ± 0.18 g L(-1) at the time (T max) of 1.49 ± 0.02 h; the absorption half-life (0.35 ± 0.04 h), elimination half-life (1.86 ± 0.24 h), the area under curve (109.81 ± 6.03 mg L(-1) h(-1)), mean residence time (3.82 ± 0.27 h), and elimination rate constant (2.26 ± 0.23 L g(-1) h(-1)) in rats vivo were derived, respectively. The possible CL mechanism of luminol-Pep-PE reaction was discussed by FI-CL, fluorescence, and molecular docking (MD) methods.
authors.affiliation: Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education, College of Chemistry & Material Science, Northwest University, Xi'an, 710069, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Luo
authors.forename: Yajuan
authors.initials: Y
authors.lastname: Li
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Zheng
authors.forename: Zhenghua
authors.initials: Z
authors.lastname: Song
mesh_terms: D000818:Animals; D001993:Bronchodilator Agents / Q000097:blood / Q000493:pharmacokinetics*; D018360:Crystallography, X-Ray; D017022:Flow Injection Analysis; D006207:Half-Life; D057230:Limit of Detection; D049449:Luminescence; D008163:Luminescent Measurements; D008165:Luminol / Q000737:chemistry*; D062105:Molecular Docking Simulation; D010434:Pepsin A / Q000737:chemistry*; D054199:Pseudoephedrine / Q000097:blood / Q000493:pharmacokinetics*; D051381:Rats; D013816:Thermodynamics
chemical_list: D001993:Bronchodilator Agents; D008165:Luminol; D054199:Pseudoephedrine; D010434:Pepsin A