title: Identification of a role for nucleolin in rabies virus infection.
abstract: Rabies virus replicates in the cytoplasm of host cells, but rabies virus phosphoprotein (P-protein) undergoes active nucleocytoplasmic trafficking. Here we show that the largely nuclear P-protein isoform P3 can localize to nucleoli and forms specific interactions with nucleolin. Importantly, depletion of nucleolin expression inhibits viral protein expression and infectious virus production by infected cells. This provides the first evidence that lyssaviruses interact with nucleolin and that nucleolin is important to lyssavirus infection.
authors.affiliation: Viral Pathogenesis Laboratory, Department of Biochemistry and Molecular Biology, Bio21 Molecular Science and Biotechnology Institute, The University of Melbourne, Melbourne, Australia Nuclear Signalling Laboratory, Department of Biochemistry and Molecular Biology, Monash University, Clayton, Australia.
authors.forename: S
authors.initials: S
authors.lastname: Oksayan
authors.affiliation: Institute for Integrative Biology of the Cell (I2BC), Université Paris-Saclay, CEA, CNRS, Université Paris-Sud, Gif-sur-Yvette, France.
authors.forename: J
authors.initials: J
authors.lastname: Nikolic
authors.affiliation: Viral Pathogenesis Laboratory, Department of Biochemistry and Molecular Biology, Bio21 Molecular Science and Biotechnology Institute, The University of Melbourne, Melbourne, Australia.
authors.forename: C T
authors.initials: CT
authors.lastname: David
authors.affiliation: Institute for Integrative Biology of the Cell (I2BC), Université Paris-Saclay, CEA, CNRS, Université Paris-Sud, Gif-sur-Yvette, France.
authors.forename: D
authors.initials: D
authors.lastname: Blondel
authors.affiliation: Nuclear Signalling Laboratory, Department of Biochemistry and Molecular Biology, Monash University, Clayton, Australia.
authors.forename: D A
authors.initials: DA
authors.lastname: Jans
authors.affiliation: Viral Pathogenesis Laboratory, Department of Biochemistry and Molecular Biology, Bio21 Molecular Science and Biotechnology Institute, The University of Melbourne, Melbourne, Australia gregory.moseley@unimelb.edu.au.
authors.forename: G W
authors.initials: GW
authors.lastname: Moseley
mesh_terms: D002460:Cell Line; D054884:Host-Pathogen Interactions; D006801:Humans; D018832:Molecular Chaperones; D010750:Phosphoproteins / Q000378:metabolism*; D025941:Protein Interaction Mapping; D016601:RNA-Binding Proteins / Q000378:metabolism*; D011820:Rabies virus / Q000502:physiology*; D015678:Viral Structural Proteins / Q000378:metabolism*; D000097796:Nucleolin
chemical_list: D018832:Molecular Chaperones; D010750:Phosphoproteins; D016601:RNA-Binding Proteins; D015678:Viral Structural Proteins; D000097796:Nucleolin; C103961:P phosphoprotein, Rabies virus