title: Lentinan reduces tumor progression by enhancing gemcitabine chemotherapy in urothelial bladder cancer.
abstract: It has been shown that chemotherapy has limited antitumor activity against advanced urothelial bladder cancer (UBC). Consequently, there is an urgent need to develop effective therapeutic methods for patients with advanced UBC. In the present study, the inhibitory effects of lentinan alone, gemcitabine alone, or lentinan combined with gemcitabine on the proliferation of the UBC cell line, T24, were investigated. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay, annexin V fluorescein isothiocyanate/propidium iodide staining, and flow cytometry were used to determine the proliferation and apoptosis of T24 cells in each treatment group. Survival-related protein expression was analyzed by western blotting. Increased concentrations of lentinan, or lentinan combined with gemcitabine, positively correlated with decreased T24 cell proliferation. Lentinan combined with gemcitabine chemotherapy significantly inhibited UBC cell proliferation. Gemcitabine has the ability to induce T24 cell apoptosis, and this effect is enhanced when it is combined with lentinan.
authors.affiliation: Department of Urology, Shengjing Hospital of China Medical University, Shenyang, 110004, Liaoning Province, PR China.
authors.forename: Ming
authors.initials: M
authors.lastname: Sun
authors.affiliation: Department of General Surgery, Shengjing Hospital of China Medical University, Shenyang, 110004, Liaoning Province, PR China.
authors.forename: Wenyan
authors.initials: W
authors.lastname: Zhao
authors.affiliation: Department of Urology, Shengjing Hospital of China Medical University, Shenyang, 110004, Liaoning Province, PR China.
authors.forename: Qingpeng
authors.initials: Q
authors.lastname: Xie
authors.affiliation: Department of Urology, Shengjing Hospital of China Medical University, Shenyang, 110004, Liaoning Province, PR China.
authors.forename: Yunhong
authors.initials: Y
authors.lastname: Zhan
authors.affiliation: Department of Urology, Shengjing Hospital of China Medical University, Shenyang, 110004, Liaoning Province, PR China. Electronic address: wubinshengjing@163.com.
authors.forename: Bin
authors.initials: B
authors.lastname: Wu
mesh_terms: D000964:Antimetabolites, Antineoplastic / Q000494:pharmacology; D000970:Antineoplastic Agents / Q000494:pharmacology*; D000971:Antineoplastic Combined Chemotherapy Protocols; D017209:Apoptosis / Q000187:drug effects; D045744:Cell Line, Tumor; D003841:Deoxycytidine / Q000031:analogs & derivatives* / Q000494:pharmacology; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007912:Lentinan / Q000494:pharmacology*; D001749:Urinary Bladder Neoplasms / Q000188:drug therapy*; D019459:Urothelium / Q000473:pathology; D000093542:Gemcitabine
chemical_list: D000964:Antimetabolites, Antineoplastic; D000970:Antineoplastic Agents; D003841:Deoxycytidine; D007912:Lentinan; D000093542:Gemcitabine
keywords: Apoptosis; Gemcitabine; Lentinan; Proliferation; Urothelial bladder cancer