title: An integrated centrifugo-opto-microfluidic platform for arraying, analysis, identification and manipulation of individual cells.
abstract: In this work we present a centrifugal microfluidic system enabling highly efficient collective trapping and alignment of particles such as microbeads and cells, their multi-colour fluorescent detection and subsequent manipulation by optical tweezers. We demonstrate array-based capture and imaging followed by "cherry-picking" of individual particles, first for fluorescently labelled polystyrene (PS) beads and then for cells. Different cell lines are discriminated based on intracellular as well as surface-based markers.
authors.affiliation: Biomedical Diagnostics Institute, National Centre for Sensor Research, School of Physical Sciences, Dublin City University, Ireland. jens.ducree@dcu.ie.
authors.forename: R
authors.initials: R
authors.lastname: Burger
authors.forename: D
authors.initials: D
authors.lastname: Kurzbuch
authors.forename: R
authors.initials: R
authors.lastname: Gorkin
authors.forename: G
authors.initials: G
authors.lastname: Kijanka
authors.forename: M
authors.initials: M
authors.lastname: Glynn
authors.forename: C
authors.initials: C
authors.lastname: McDonagh
authors.forename: J
authors.initials: J
authors.lastname: Ducrée
mesh_terms: D000951:Antigens, Neoplasm / Q000378:metabolism; D015815:Cell Adhesion Molecules / Q000378:metabolism; D002469:Cell Separation / Q000295:instrumentation / Q000379:methods*; D002498:Centrifugation; D000071858:Epithelial Cell Adhesion Molecule; D004867:Equipment Design; D016650:Fluorescein-5-isothiocyanate / Q000737:chemistry; D018922:HL-60 Cells; D006367:HeLa Cells; D006801:Humans; D061986:MCF-7 Cells; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D008856:Microscopy, Fluorescence; D008863:Microspheres; D052898:Optical Tweezers; D011137:Polystyrenes / Q000737:chemistry
chemical_list: D000951:Antigens, Neoplasm; D015815:Cell Adhesion Molecules; C499379:EPCAM protein, human; D000071858:Epithelial Cell Adhesion Molecule; D011137:Polystyrenes; D016650:Fluorescein-5-isothiocyanate