title: Plasma CC-chemokine ligand 28 level is correlated with hematopoietic stem cells in human cord blood.
abstract: BACKGROUND
  CC-chemokine ligand 28 (CCL28) was previously identified as a novel growth factor in vitro for hematopoietic stem cells (HSCs) from cord blood (CB). However, there is no report on the relationship between CCL28 and HSCs in a human body.
  
  
  METHODS
  To reveal the effect of CCL28 on hematopoietic cells in human CB at birth, we measured CCL28 in frozen CB plasma, which was preserved as a reference sample for cryopreserved CB units for HSC transplantation. We also evaluated the correlation of CCL28 level with CB components.
  
  
  RESULTS
  A total of 81 cryopreserved nonconforming CB units for transplantation were selected. The level of CCL28 was 2540 ± 377 pg/mL. The CCL28 levels correlated with the number of CD34+ cells (r = 0.222, p = 0.047) and white blood cells (r = 0.254, p = 0.022) in the CB units. The CCL28 levels also correlated with hemoglobin levels (r = 0.221, p = 0.048) in fresh CB.
  
  
  CONCLUSIONS
  This finding of positive correlation between CCL28 level and CD34+ cell numbers in vivo, together with the previous report that CCL28 influences the proliferation of hematopoietic cells in CB in vitro, may give a clue for better understanding the variability in HSC content in CB that is cryopreserved for HSC transplantation.
authors.affiliation: Department of Laboratory Medicine, Seoul National University College of Medicine, Seoul, Korea.|Department of Laboratory Medicine, Boramae Hospital, Seoul, Korea.|Seoul Metropolitan Government Public Cord Blood Bank (Allcord), Seoul, Korea.
authors.forename: Jong Hyun
authors.initials: JH
authors.lastname: Yoon
authors.affiliation: Biostatistics, Boramae Hospital, Seoul, Korea.
authors.forename: Sohee
authors.initials: S
authors.lastname: Oh
authors.affiliation: Department of Laboratory Medicine, Seoul National University College of Medicine, Seoul, Korea.|Department of Laboratory Medicine, Boramae Hospital, Seoul, Korea.|Seoul Metropolitan Government Public Cord Blood Bank (Allcord), Seoul, Korea.
authors.forename: Sue
authors.initials: S
authors.lastname: Shin
authors.affiliation: Department of Laboratory Medicine, Seoul National University College of Medicine, Seoul, Korea.|Department of Laboratory Medicine, Boramae Hospital, Seoul, Korea.|Seoul Metropolitan Government Public Cord Blood Bank (Allcord), Seoul, Korea.
authors.forename: Eun Youn
authors.initials: EY
authors.lastname: Roh
authors.affiliation: Department of Laboratory Medicine, Gyeongsang National University Hospital, Jinju, Korea.
authors.forename: Hye Ryun
authors.initials: HR
authors.lastname: Lee
authors.affiliation: Department of Laboratory Medicine, Seoul National University College of Medicine, Seoul, Korea.
authors.forename: Soo Hyun
authors.initials: SH
authors.lastname: Seo
authors.affiliation: Department of Laboratory Medicine, Seoul National University College of Medicine, Seoul, Korea.
authors.forename: Hyunwoong
authors.initials: H
authors.lastname: Park
authors.affiliation: Department of Laboratory Medicine, Seoul National University College of Medicine, Seoul, Korea.
authors.forename: Eun Young
authors.initials: EY
authors.lastname: Song
mesh_terms: D018952:Antigens, CD34 / Q000378:metabolism; D049109:Cell Proliferation / Q000502:physiology; D019742:Chemokines, CC / Q000097:blood*; D015925:Cryopreservation; D004797:Enzyme-Linked Immunosorbent Assay; D005312:Fetal Blood / Q000378:metabolism*; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000378:metabolism; D006412:Hematopoietic Stem Cells / Q000378:metabolism*; D006801:Humans
chemical_list: D018952:Antigens, CD34; C414047:CCL28 protein, human; D019742:Chemokines, CC; D016178:Granulocyte-Macrophage Colony-Stimulating Factor