title: The level of viral infection of antigen-presenting cells correlates with the level of development of Theiler's murine encephalomyelitis virus-induced demyelinating disease.
abstract: Intracerebral infection with Theiler's murine encephalomyelitis virus (TMEV) induces immune-mediated demyelinating disease in susceptible SJL/J mice but not in resistant C57BL/6 mice. Previous studies have indicated that the major histocompatibility complex (MHC) genes play the most prominent role in the development of TMEV-induced demyelinating disease. In this study, we used C57BL/6.S (B6.S) congenic mice, which carry H-2(s) MHC genes instead of H-2(b) MHC genes in conjunction with the C57BL/6 (B6) background genes. Our data show that virus-infected B6.S mice are free from disease and have significantly lower viral loads than susceptible SJL mice, particularly in the spinal cord. A strong protective Th1-type T helper response with virtually no pathogenic Th17 response was detected in B6.S mice, in contrast to the reduced Th1- and robust Th17-type responses in SJL mice. Notably, lower levels of viral infectivity in B6.S antigen-presenting cells (APCs) correlated with the disease resistance and T-cell-type response. In vitro studies using APCs from B6.S and SJL mice show that TLR2, -3, -4, and -7, but not TLR9, signaling can replace viral infection and augment the effect of viral infection in the differentiation of the pathogenic Th17 cell type. Taken together, these results strongly suggest that the viral replication levels in APCs critically affect the induction of protective versus pathogenic Th cell types via the signaling of pattern recognition receptors for innate immune responses. Our current findings further imply that the levels of viral infectivity/replication and TLR-mediated signaling play critical roles in the pathogenesis of chronic viral diseases.
  
  
  OBJECTIVE
  This study indicates that innate immune cytokines produced in antigen-presenting cells stimulating the T cell immune responses during early viral infection play a critical role in determining the susceptibility of mice to the development of demyelinating disease. The level of innate immune cytokines reflects the level of initial viral infection in the antigen-presenting cells, and the level determines the development of T cell types, which are either protective or pathogenic. The level of initial viral infection to the cells is controlled by a gene or genes that are not associated with the major histocompatibility antigen complex genes. This finding has an important implication in controlling not only chronic viral infections but also infection-induced autoimmune-like diseases, which are closely associated with the pathogenic type of T cell responses.
authors.affiliation: Department of Microbiology-Immunology, Northwestern University Medical School, Chicago, Illinois, USA.
authors.forename: Young Hee
authors.initials: YH
authors.lastname: Jin
authors.affiliation: Department of Microbiology-Immunology, Northwestern University Medical School, Chicago, Illinois, USA.
authors.forename: Hyun Seok
authors.initials: HS
authors.lastname: Kang
authors.affiliation: Department of Microbiology-Immunology, Northwestern University Medical School, Chicago, Illinois, USA.
authors.forename: Wanqiu
authors.initials: W
authors.lastname: Hou
authors.affiliation: Department of Microbiology-Immunology, Northwestern University Medical School, Chicago, Illinois, USA.
authors.forename: Liping
authors.initials: L
authors.lastname: Meng
authors.affiliation: Department of Microbiology-Immunology, Northwestern University Medical School, Chicago, Illinois, USA Department of Pathology, Northwestern University Medical School, Chicago, Illinois, USA bskim@northwestern.edu.
authors.forename: Byung S
authors.initials: BS
authors.lastname: Kim
mesh_terms: D000818:Animals; D000938:Antigen-Presenting Cells / Q000276:immunology / Q000821:virology*; D018188:Cardiovirus Infections / Q000276:immunology / Q000473:pathology* / Q000821:virology*; D003711:Demyelinating Diseases / Q000276:immunology / Q000473:pathology* / Q000821:virology*; D005260:Female; D005805:Genes, MHC Class I; D008810:Mice, Inbred C57BL; D018417:Th1 Cells / Q000276:immunology; D058504:Th17 Cells / Q000276:immunology; D017945:Theilovirus / Q000276:immunology / Q000302:isolation & purification*; D051193:Toll-Like Receptors / Q000276:immunology; D019562:Viral Load
chemical_list: D051193:Toll-Like Receptors