title: Effects of diabetes on oxidative and nitrosative stress in kidney mitochondria from aged rats.
abstract: Diabetes mellitus (DM) is characterized by chronic hyperglycemia resulting from defects in the secretion and/or action of insulin. Diabetic nephropathy (DN) develops in diabetic patients and is characterized by a progressive deterioration of renal function. The mitochondrial electron transport chain (ETC) produces most of the reactive oxygen species (ROS) that are involved in diabetic nephropathy. Due to the high incidence of DM in the elderly, the aim of this study was to evaluate oxidative and nitrosative stress in kidney mitochondria from aged rats. We evaluated lipid peroxidation (LPO), nitric oxide (NO(•)) production, S-nitrosylation profiles, glutathione levels, and glutathione reductase and aconitase activities under streptozotocin (STZ)-induced experimental diabetes in kidney mitochondria from aged rats. The results showed an increase in LPO, NO(•) production, and S-nitrosylated proteins in rats with STZ-induced diabetes. A decrease in glutathione (GSH) levels and glutathione reductase (GR) and aconitase activities in the rats that received the STZ-induced diabetes treatment was also observed, when compared with the age-related controls. The data suggest that oxidative and nitrosative stresses promote mitochondrial oxidative dysfunction in the more advanced age rat kidney in STZ-induced diabetes.
authors.affiliation: Instituto de Investigaciones Químico-Biológicas, Universidad Michoacana de San Nicolás de Hidalgo, Edificio B-3. C.U., Morelia, Mich, 58030, Mexico.
authors.forename: Rocío V
authors.initials: RV
authors.lastname: Pérez-Gallardo
authors.forename: Ruth
authors.initials: R
authors.lastname: Noriega-Cisneros
authors.forename: Edgar
authors.initials: E
authors.lastname: Esquivel-Gutiérrez
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Calderón-Cortés
authors.forename: Christian
authors.initials: C
authors.lastname: Cortés-Rojo
authors.forename: Salvador
authors.initials: S
authors.lastname: Manzo-Avalos
authors.forename: Jesús
authors.initials: J
authors.lastname: Campos-García
authors.forename: Rafael
authors.initials: R
authors.lastname: Salgado-Garciglia
authors.forename: Rocío
authors.initials: R
authors.lastname: Montoya-Pérez
authors.forename: Istvan
authors.initials: I
authors.lastname: Boldogh
authors.forename: Alfredo
authors.initials: A
authors.lastname: Saavedra-Molina
mesh_terms: D000375:Aging; D000818:Animals; D003921:Diabetes Mellitus, Experimental / Q000235:genetics* / Q000378:metabolism*; D007668:Kidney / Q000378:metabolism*; D008297:Male; D008928:Mitochondria / Q000378:metabolism*; D009569:Nitric Oxide / Q000378:metabolism*; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D051381:Rats; D017208:Rats, Wistar; D017382:Reactive Oxygen Species
chemical_list: D017382:Reactive Oxygen Species; D009569:Nitric Oxide