title: Stoichiometric expression of MMP-2/TIMP-2 in benign and malignant tumours of the salivary gland.
abstract: The aim of this study was to determine the expression of matrix metalloproteinase 2 (MMP-2) and tissue inhibitors of matrix metalloproteinase 2 (TIMP-2) and the MMP-2/TIMP-2 expression ratio in salivary gland tumours (SGTs). Forty-three FFPE SGTs were prepared for antibody processing to MMP-2 and TIMP-2. Two investigators utilizing Sinicrope's method scored the uptake of immuno-stains. Cytoplasmic staining was considered as positive. Data was analysed using SPSS version 20. The significance level was set at p < 0.05. In benign SGTs, the mean score for MMP-2 was not significantly lower than that of TIMP-2 (p = 0.37). However, the mean scores for MMP-2 stain intensity and proportion were significantly higher in malignant than benign SGTs (p = 0.01 and p = 0.02 respectively). There was no significant difference in the mean MMP-2/TIMP-2 expression ratio of the malignant SGTs according to histological grade and histogenesis (p = 0.4 and p = 0.19 respectively). The MMP-2/TIMP-2 expression ratio has a higher prognostic value than the separate expressions of MMP-2 and TIMP-2.
authors.affiliation: Department of Oral Pathology, Faculty of Dentistry, College of Medicine, University of Ibadan, Ibadan, Oyo State, Nigeria, delekolude2003@yahoo.co.uk.
authors.forename: Bamidele
authors.initials: B
authors.lastname: Kolude
authors.forename: Akinyele Olumuyiwa
authors.initials: AO
authors.lastname: Adisa
authors.forename: Ahmed Oluwatoyin
authors.initials: AO
authors.lastname: Lawal
authors.forename: Bukola Folasade
authors.initials: BF
authors.lastname: Adeyemi
authors.forename: Akindayo Olufunto
authors.initials: AO
authors.lastname: Akinyamoju
mesh_terms: D000328:Adult; D000368:Aged; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D020778:Matrix Metalloproteinase 2 / Q000096:biosynthesis*; D020780:Matrix Metalloproteinase 9 / Q000096:biosynthesis; D008875:Middle Aged; D011379:Prognosis; D012468:Salivary Gland Neoplasms / Q000235:genetics* / Q000473:pathology; D019716:Tissue Inhibitor of Metalloproteinase-2 / Q000096:biosynthesis*
chemical_list: C560823:TIMP2 protein, human; D019716:Tissue Inhibitor of Metalloproteinase-2; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9