title: Evaluation of various silicon-and boron-containing compounds for the detection of phosphorylation in peptides via gas-phase ion-molecule reactions.
abstract: Gas-phase ion-molecule reactions [IMR] of various boron- and silicon-containing neutrals were investigated as a potential route for detecting phosphorylation within peptides in the negative ion mode. Trimethyl borate (TMB), triethyl borate (TEB) and N,O- Bis(trimethylsilyl)acetamide (TMSA), unlike diethylmethoxyborane (DEMB), diisopropoxymethylborane [DiPMB] and chlorotrimethylsi- Lane (TMSCIL], reacted differently if a phosphate moiety was present and thus are suitable to detect phosphorylation. During multistage collision-induced dissociation experiments of the reaction products of IMR with TMB and TEB, the [LSsF - 4H + B]- ion formed a modified y2 fragment allowing the phosphorylation site to be assigned, unlike reaction products of DEMB and DiPMB which lost both the phos- phoric acid and the boron-containing moiety.
authors.forename: Andrii
authors.initials: A
authors.lastname: Piatkivskyi
authors.forename: Yuriy
authors.initials: Y
authors.lastname: Pyatkivskyy
authors.forename: Victor
authors.initials: V
authors.lastname: Ryzhov
mesh_terms: D001881:Borates / Q000737:chemistry; D001896:Boron Compounds / Q000737:chemistry*; D005740:Gases / Q000737:chemistry; D013058:Mass Spectrometry / Q000379:methods*; D010455:Peptides / Q000032:analysis* / Q000737:chemistry* / Q000378:metabolism; D010748:Phosphopeptides / Q000032:analysis / Q000737:chemistry; D010766:Phosphorylation; D012825:Silicon / Q000737:chemistry*
chemical_list: D001881:Borates; D001896:Boron Compounds; D005740:Gases; D010455:Peptides; D010748:Phosphopeptides; C102081:trimethyl borate; D012825:Silicon