title: SPOT14-positive neural stem/progenitor cells in the hippocampus respond dynamically to neurogenic regulators.
abstract: Proliferation of neural stem/progenitor cells (NSPCs) in the adult brain is tightly controlled to prevent exhaustion and to ensure proper neurogenesis. Several extrinsic stimuli affect NSPC regulation. However, the lack of unique markers led to controversial results regarding the in vivo behavior of NSPCs to different stimuli. We recently identified SPOT14, which controls NSPC proliferation through regulation of de novo lipogenesis, selectively in low-proliferating NSPCs. Whether SPOT14-expressing (SPOT14+) NSPCs react in vivo to neurogenic regulators is not known. We show that aging is accompanied by a marked disappearance of SPOT14+ NSPCs, whereas running, a positive neurogenic stimulus, increases proliferation of SPOT14+ NSPCs. Furthermore, transient depletion of highly proliferative cells recruits SPOT14+ NSPCs into the proliferative pool. Additionally, we have established endogenous SPOT14 protein staining, reflecting transgenic SPOT14-GFP expression. Thus, our data identify SPOT14 as a potent marker for adult NSPCs that react dynamically to positive and negative neurogenic regulators.
authors.affiliation: Brain Research Institute, Faculty of Medicine, University of Zurich, 8057 Zurich, Switzerland; Neuroscience Center Zurich, University of Zurich and ETH Zurich, 8057 Zurich, Switzerland.
authors.forename: Marlen
authors.initials: M
authors.lastname: Knobloch
authors.affiliation: Brain Research Institute, Faculty of Medicine, University of Zurich, 8057 Zurich, Switzerland; Neuroscience Center Zurich, University of Zurich and ETH Zurich, 8057 Zurich, Switzerland.
authors.forename: Carolin
authors.initials: C
authors.lastname: von Schoultz
authors.affiliation: Brain Research Institute, Faculty of Medicine, University of Zurich, 8057 Zurich, Switzerland; Neuroscience Center Zurich, University of Zurich and ETH Zurich, 8057 Zurich, Switzerland.
authors.forename: Luis
authors.initials: L
authors.lastname: Zurkirchen
authors.affiliation: Brain Research Institute, Faculty of Medicine, University of Zurich, 8057 Zurich, Switzerland; Neuroscience Center Zurich, University of Zurich and ETH Zurich, 8057 Zurich, Switzerland.
authors.forename: Simon M G
authors.initials: SM
authors.lastname: Braun
authors.affiliation: Brain Research Institute, Faculty of Medicine, University of Zurich, 8057 Zurich, Switzerland.
authors.forename: Mojca
authors.initials: M
authors.lastname: Vidmar
authors.affiliation: Brain Research Institute, Faculty of Medicine, University of Zurich, 8057 Zurich, Switzerland; Neuroscience Center Zurich, University of Zurich and ETH Zurich, 8057 Zurich, Switzerland. Electronic address: jessberger@hifo.uzh.ch.
authors.forename: Sebastian
authors.initials: S
authors.lastname: Jessberger
mesh_terms: D000367:Age Factors; D000818:Animals; D018906:Antineoplastic Agents, Alkylating / Q000494:pharmacology; D015415:Biomarkers / Q000378:metabolism; D049109:Cell Proliferation / Q000187:drug effects; D003606:Dacarbazine / Q000031:analogs & derivatives / Q000494:pharmacology; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D006624:Hippocampus / Q000166:cytology / Q000254:growth & development / Q000378:metabolism*; D007150:Immunohistochemistry; D008822:Mice, Transgenic; D008856:Microscopy, Fluorescence; D058953:Neural Stem Cells / Q000378:metabolism*; D055495:Neurogenesis / Q000187:drug effects; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D000077204:Temozolomide; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: D018906:Antineoplastic Agents, Alkylating; D015415:Biomarkers; D009687:Nuclear Proteins; C501477:Thrsp protein, mouse; D014157:Transcription Factors; D049452:Green Fluorescent Proteins; D003606:Dacarbazine; D000077204:Temozolomide