title: Endophytic fungi from the genus Colletotrichum are abundant in the Phaseolus vulgaris and have high genetic diversity.
abstract: OBJECTIVE
  To evaluate the diversity of endophytic fungi from the leaves of the common bean and the genetic diversity of endophytic fungi from the genus Colletotrichum using IRAP (inter-retrotransposon amplified polymorphism) and REMAP (retrotransposon-microsatellite amplified polymorphism) analyses.
  
  
  RESULTS
  The fungi were isolated by tissue fragmentation and identified by analysing the morphological features and sequencing the internal transcribed spacer (ITS) regions and the rDNA large subunit (LSU). Twenty-seven different taxa were identified. Colletotrichum was the most commonly isolated genera from the common bean (32.69% and 24.29% of the total isolates from the Ouro Negro and Talismã varieties, respectively). The IRAP and REMAP analyses revealed a high genetic diversity in the Colletotrichum endophytic isolates and were able to discriminate these isolates from the phytopathogen Colletotrichum lindemuthianum.
  
  
  CONCLUSIONS
  Fungi from the genus Colletotrichum are abundant in the Phaseolus vulgaris endophytic community, and the IRAP and REMAP markers can be used to rapidly distinguish between C. lindemuthianum and other Colletotrichum members that are frequently found as endophytes.
  
  
  CONCLUSIONS
  This is the first report of the diversity of endophytic fungi present in the common bean and the use of IRAP and REMAP markers to assess the genetic diversity of endophytic fungi from the genus Colletotrichum.
authors.affiliation: Department of Microbiology, Universidade Federal de Viçosa, Viçosa, Brazil.
authors.forename: L L
authors.initials: LL
authors.lastname: Gonzaga
authors.forename: L E O
authors.initials: LE
authors.lastname: Costa
authors.forename: T T
authors.initials: TT
authors.lastname: Santos
authors.forename: E F
authors.initials: EF
authors.lastname: Araújo
authors.forename: M V
authors.initials: MV
authors.lastname: Queiroz
mesh_terms: D020231:Colletotrichum / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D060026:Endophytes / Q000235:genetics; D005658:Fungi / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D014644:Genetic Variation; D018895:Microsatellite Repeats; D027805:Phaseolus / Q000382:microbiology*; D018515:Plant Leaves / Q000382:microbiology; D011110:Polymorphism, Genetic; D018626:Retroelements
chemical_list: D018626:Retroelements
keywords: Colletotrichum; Phaseolus vulgaris; endophytic fungi; inter-retrotransposon amplified polymorphism; internal transcribed spacer; large subunit; retrotransposon-microsatellite amplified polymorphism