title: Iron speciation by microsequential injection solid phase spectrometry using 3-hydroxy-1(H)-2-methyl-4-pyridinone as chromogenic reagent.
abstract: The speciation of iron using the newly synthesized 3-hydroxy-1(H)-2-methyl-4-pyridinone by solid phase spectrophotometry in a microsequential injection lab-on-valve (µSI-LOV-SPS) methodology is described. Iron was retained in a reusable column, Nitrilotriacetic Acid Superflow (NTA) resin, and the ligand was used as both chromogenic and eluting reagent. This approach, analyte retention and matrix removal, enabled the assessment of iron (III) and total iron content in fresh waters and high salinity coastal waters with direct sample introduction, in the range of 20.0-100 µg/L. with a LOD of 9 µg/L. The overall effluent production was 2 mL, corresponding to the consumption of 0.48 µg of 2-metil-3-hydroxy-4-pyridinone, 0.34 mg of NaHCO3, 16 mg of HNO3, 4.4 µg H2O2 and 400µL of sample. Four reference samples were analyzed and a relative deviation<10% was obtained; furthermore, several bathing waters (♯13) were analyzed using the developed method and the results were comparable to those obtained by atomic absorption spectrophotometry (relative deviations<6%).
authors.affiliation: Group of Analytical Chemistry, Automation, and Environment, Department of Chemistry, University of the Balearic Islands, Carreterra de Valldemossa km 7.5, 07122 Palma de Mallorca, Spain.
authors.forename: Ruth
authors.initials: R
authors.lastname: Suárez
authors.affiliation: CBQF - Centro de Biotecnologia e Química Fina - Laboratório Associado, Escola Superior de Biotecnologia, Universidade Católica Portuguesa/Porto, Rua Dr. António Bernardino Almeida, 4200-072 Porto, Portugal; Laboratório de Hidrobiologia, Instituto de Ciências Biomédicas Abel Salazar (ICBAS) and Instituto de Investigação Marinha (CIIMAR), Universidade do Porto, Rua de Jorge Viterbo Ferreira no. 228, 4050-313 Porto, Portugal. Electronic address: rmesquita@porto.ucp.pt.
authors.forename: Raquel B R
authors.initials: RB
authors.lastname: Mesquita
authors.affiliation: REQUIMTE, Instituto de Ciências Biomédicas Abel Salazar (ICBAS), Rua de Jorge Viterbo Ferreira no. 228, 4050-313 Porto, Portugal.
authors.forename: Maria
authors.initials: M
authors.lastname: Rangel
authors.affiliation: Group of Analytical Chemistry, Automation, and Environment, Department of Chemistry, University of the Balearic Islands, Carreterra de Valldemossa km 7.5, 07122 Palma de Mallorca, Spain.
authors.forename: Víctor
authors.initials: V
authors.lastname: Cerdà
authors.affiliation: CBQF - Centro de Biotecnologia e Química Fina - Laboratório Associado, Escola Superior de Biotecnologia, Universidade Católica Portuguesa/Porto, Rua Dr. António Bernardino Almeida, 4200-072 Porto, Portugal.
authors.forename: António O S S
authors.initials: AO
authors.lastname: Rangel
mesh_terms: D004867:Equipment Design; D017022:Flow Injection Analysis / Q000295:instrumentation; D005618:Fresh Water / Q000032:analysis*; D057230:Limit of Detection; D008745:Methylation; D008958:Models, Molecular; D009571:Nitrilotriacetic Acid / Q000737:chemistry; D009792:Oceans and Seas; D011728:Pyridones / Q000737:chemistry*; D013053:Spectrophotometry / Q000295:instrumentation*
chemical_list: D011728:Pyridones; C017679:2-hydroxypyridine; D009571:Nitrilotriacetic Acid
keywords: 3-Hydroxy-4-pyridinone; Iron(III); Lab-on-valve; NTA resin; Solid phase spectrometry; Total iron