title: Separation mechanisms for palonosetron stereoisomers at different chiral selector concentrations in MEKC.
abstract: The separation mechanisms for palonosetron (PALO) stereoisomers in MEKC using sodium cholate (SC) as surfactant and chiral selector have been studied, in a wide range of concentrations below and above the CMC. It was found that SC micelles only provide chirally selective recognition for 3a carbon chiral center in PALO molecules. The resolution of the configurations of 2 carbon chiral center is achieved by the difference of mobility in continuous phase. A schematic diagram depicting the separation mechanisms and the corresponding migration orders among all of four stereoisomers was proposed based on the measured separation parameters. A MEKC method to achieve the complete separation of four stereoisomers in very short time using a very low chiral selector concentration, instead of high concentrations generally considered, was developed based on the understanding of the mechanisms.
authors.affiliation: College of Chemistry and Chemical Engineering, Luoyang Normal University, Luoyang, China.
authors.forename: Shao-Qiang
authors.initials: SQ
authors.lastname: Hu
authors.forename: Xu-Ming
authors.initials: XM
authors.lastname: Guo
authors.forename: Hai-Jun
authors.initials: HJ
authors.lastname: Shi
authors.forename: Rong-Jie
authors.initials: RJ
authors.lastname: Luo
mesh_terms: D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D007546:Isoquinolines / Q000737:chemistry* / Q000302:isolation & purification*; D000077924:Palonosetron; D011812:Quinuclidines / Q000737:chemistry* / Q000302:isolation & purification*; D020358:Sodium Cholate / Q000737:chemistry; D013237:Stereoisomerism; D013501:Surface-Active Agents / Q000737:chemistry
chemical_list: D007546:Isoquinolines; D011812:Quinuclidines; D013501:Surface-Active Agents; D000077924:Palonosetron; D020358:Sodium Cholate
keywords: Chiral separation; MEKC; Palonosetron hydrochloride; Separation mechanisms; Sodium cholate