title: Dual-functional probes for sequential thiol and redox homeostasis sensing in live cells.
abstract: A new type of resorufin-based dual-functional fluorescent probe whose fluorescence emission features are sensitive to thiol compounds and redox homeostasis was developed. Thiols-triggered nucleophilic substitution of the probes converts the nonfluorescent probe to the highly fluorescent resorufin moiety; the released resorufin not only enables fluorescence signaling specific for thiol compounds but functions as a redox indicator with sensitive colorimetric and fluorescence emission change upon redox variation. Preliminary fluorescence imaging experiments have revealed the biocompatibility of the as-prepared probes and validated their practicability for thiol sensing and redox homeostasis mapping in living cells.
authors.affiliation: School of Chemistry and Chemical Engineering, University of Chinese Academy of Sciences (UCAS), Beijing 100049, P. R. China. zytian@ucas.ac.cn.
authors.forename: Tao
authors.initials: T
authors.lastname: Ma
authors.forename: Hui
authors.initials: H
authors.lastname: Ding
authors.forename: Haijiao
authors.initials: H
authors.lastname: Xu
authors.forename: Yanlin
authors.initials: Y
authors.lastname: Lv
authors.forename: Heng
authors.initials: H
authors.lastname: Liu
authors.forename: Hongda
authors.initials: H
authors.lastname: Wang
authors.forename: Zhiyuan
authors.initials: Z
authors.lastname: Tian
mesh_terms: D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D006706:Homeostasis; D006801:Humans; D008264:Macrophages / Q000737:chemistry* / Q000166:cytology / Q000378:metabolism; D015394:Molecular Structure; D010078:Oxazines / Q000138:chemical synthesis / Q000737:chemistry*; D010084:Oxidation-Reduction; D013438:Sulfhydryl Compounds / Q000032:analysis* / Q000378:metabolism
chemical_list: D005456:Fluorescent Dyes; D010078:Oxazines; D013438:Sulfhydryl Compounds; C014180:resorufin