title: Multicommutated stepwise injection determination of ascorbic acid in medicinal plants and food samples by capillary zone electrophoresis ultraviolet detection.
abstract: An automation of the extraction of analytes from solid samples into the aqueous phase based on multicommutated stepwise injection analysis concept has been suggested. The feasibility of the approach has been demonstrated by determination of ascorbic acid as model analyte. The method includes automated extraction of ascorbic acid from solid sample into borate buffer solution pH 8 in mixing chamber during vigorous mixing by nitrogen stream, and subsequent detection by capillary zone electrophoresis at 254 nm. The method has a linear range of 0.1-5.0 mg g(-1) for ascorbic acid with the LOD of 0.03 mg g(-1). The sample throughput was 7 h(-1). This method was applied for determination of ascorbic acid in various medicinal plants and food samples.
authors.affiliation: Department of Analytical Chemistry, Faculty of Chemistry, Saint Petersburg State University, 198504 Saint Petersburg, Russia.
authors.forename: Marina T
authors.initials: MT
authors.lastname: Falkova
authors.affiliation: Department of Analytical Chemistry, Faculty of Chemistry, Saint Petersburg State University, 198504 Saint Petersburg, Russia. Electronic address: bulatov_andrey@mail.ru.
authors.forename: Andrey V
authors.initials: AV
authors.lastname: Bulatov
authors.affiliation: Department of Analytical Chemistry, Faculty of Chemistry, Saint Petersburg State University, 198504 Saint Petersburg, Russia.
authors.forename: Maria O
authors.initials: MO
authors.lastname: Pushina
authors.affiliation: Department of Analytical Chemistry, Saint Petersburg State Chemical-Pharmaceutical Academy, 197376 Saint Petersburg, Russia.
authors.forename: Aleksey A
authors.initials: AA
authors.lastname: Ekimov
authors.affiliation: Department of Analytical Chemistry, Saint Petersburg State Chemical-Pharmaceutical Academy, 197376 Saint Petersburg, Russia.
authors.forename: Galina M
authors.initials: GM
authors.lastname: Alekseeva
authors.affiliation: Department of Analytical Chemistry, Faculty of Chemistry, Saint Petersburg State University, 198504 Saint Petersburg, Russia.
authors.forename: Leonid N
authors.initials: LN
authors.lastname: Moskvin
mesh_terms: D001205:Ascorbic Acid / Q000032:analysis*; D019075:Electrophoresis, Capillary / Q000295:instrumentation*; D017022:Flow Injection Analysis / Q000295:instrumentation; D057230:Limit of Detection; D010946:Plants, Medicinal / Q000737:chemistry*; D015203:Reproducibility of Results
chemical_list: D001205:Ascorbic Acid
keywords: Automation; Capillary zone electrophoresis; Food; Medicinal plants; Multicommutated stepwise injection analysis