title: Improved ROS measurement in root hair cells.
abstract: Reactive oxygen species (ROS) are recognized as important signaling components in various processes in plants. ROS are produced for NADPH oxidase in different subcellular compartments and they are involved for a wide range of stimuli, such as cell cycle, growth, plant defenses, abiotic stress responses, and abscisic acid signaling in guard cells. In Arabidopsis, root hairs ROS also play a key role in root hair growth and they control the activity of calcium channels required for polar growth (Takeda et al. Science 319:1241-1244, 2008). The production of reactive oxygen species is under a specific molecular control in order to avoid detrimental side effects. Here we describe a protocol to detect ROS by oxidation of a derivative of fluorescein: 2',7-dihidro dicloro fluorescein (H2DCFDA).
authors.affiliation: Laboratorio de Fisiología y Biología Molecular, IFIByNE (CONICET), FCEyN, Universidad de Buenos Aires, Pab.II, Ciudad, Universitaria, Intendente Güiraldes 2160, Buenos Aires, C1428EGA, Argentina.
authors.forename: Silvina Paola Denita
authors.initials: SP
authors.lastname: Juárez
authors.forename: Silvina
authors.initials: S
authors.lastname: Mangano
authors.forename: José M
authors.initials: JM
authors.lastname: Estevez
mesh_terms: D017360:Arabidopsis / Q000166:cytology* / Q000201:enzymology; D029681:Arabidopsis Proteins / Q000378:metabolism; D003600:Cytosol / Q000378:metabolism; D005452:Fluoresceins / Q000378:metabolism*; D005456:Fluorescent Dyes / Q000378:metabolism*; D019255:NADPH Oxidases / Q000378:metabolism; D059828:Plant Cells / Q000378:metabolism; D018517:Plant Roots / Q000166:cytology* / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology
chemical_list: C109098:2,7-dihydrodichlorofluorescein diacetate; D029681:Arabidopsis Proteins; D005452:Fluoresceins; D005456:Fluorescent Dyes; D017382:Reactive Oxygen Species; D019255:NADPH Oxidases