title: [ITF increases the transcriptional activity of ITF promoter via the JAK-STAT3 signal transduction pathway].
abstract: OBJECTIVE
  To investigate the eff ect of intestinal trefoil factor (ITF) on the transcriptional activity of ITF promoter and to explore the regulatory mechanism of Janus kinase/signal transducers and activators of transcription (JAK/STAT) on ITF promoter.
  
  
  METHODS
  The 5' flanking sequence of the ITF gene was cloned from human whole blood genomic DNA by PCR. ITF promoter fragment was cloned and inserted into the pGL3-Basic vector to construct recombinant vector. ITF promoter vector was stimulated with ITF at various concentrations and the luciferase activity was measured. The JAK-STAT3 signal transduction pathway was then blocked by a specific inhibitor AG490 to determine the signal pathway involved in ITF promoter activity.
  
  
  RESULTS
  Restriction endonuclease analysis and DNA sequencing confirmed that the recombinant plasmid, containing ITF promoter, was constructed successfully. After transient transfection, the activity of ITF promoter was increased significantly in the presence of ITF (P<0.05). Blockage of the JAK-STAT3 signal transduction pathway with AG490 significantly reduced the ITF promoter activity (P<0.05).
  
  
  CONCLUSIONS
  ITF increases the transcriptional activity of ITF promoter via the JAK-STAT3 signal transduction pathway.
authors.affiliation: Department of Burn Surgery, Huaihai Hospital Affi liated to Xuzhou Medical College, Xuzhou Jiangshu 221004; Department of Burn Surgery, the No. 97 Hospital of PLA, Xuzhou Jiangshu 221004, China sunyong_97@sina.com.
authors.forename: Yong
authors.initials: Y
authors.lastname: Sun
authors.forename: Juan
authors.initials: J
authors.lastname: LE
authors.forename: Baojun
authors.initials: B
authors.lastname: Chen
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Pan
authors.forename: Fang
authors.initials: F
authors.lastname: Zhang
mesh_terms: D006801:Humans; D053612:Janus Kinases / Q000378:metabolism*; D010455:Peptides / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D050796:STAT3 Transcription Factor / Q000378:metabolism*; D015398:Signal Transduction; D014162:Transfection; D000071164:Trefoil Factor-2; D020032:Tyrphostins
chemical_list: D010455:Peptides; D050796:STAT3 Transcription Factor; C494086:STAT3 protein, human; D000071164:Trefoil Factor-2; D020032:Tyrphostins; C095512:alpha-cyano-(3,4-dihydroxy)-N-benzylcinnamide; D053612:Janus Kinases