title: Engineered collagen hydrogels for the sustained release of biomolecules and imaging agents: promoting the growth of human gingival cells.
abstract: We present here the in vitro release profiles of either fluorescently labeled biomolecules or computed tomography contrast nanoagents from engineered collagen hydrogels under physiological conditions. The collagen constructs were designed as potential biocompatible inserts into wounded human gingiva. The collagen hydrogels were fabricated under a variety of conditions in order to optimize the release profile of biomolecules and nanoparticles for the desired duration and amount. The collagen constructs containing biomolecules/nanoconstructs were incubated under physiological conditions (ie, 37°C and 5% CO2) for 24 hours, and the release profile was tuned from 20% to 70% of initially loaded materials by varying the gelation conditions of the collagen constructs. The amounts of released biomolecules and nanoparticles were quantified respectively by measuring the intensity of fluorescence and X-ray scattering. The collagen hydrogel we fabricated may serve as an efficient platform for the controlled release of biomolecules and imaging agents in human gingiva to facilitate the regeneration of oral tissues.
authors.affiliation: Department of Bionanotechnology, Hanyang University, Seoul Campus, Seoul, Korea ; Department of Bionanoengineering, Hanyang University, ERICA Campus, Ansan, Korea.
authors.forename: Jonghoon
authors.initials: J
authors.lastname: Choi
authors.affiliation: Center for Biomaterials, Biomedical Research Institute, Korea Institute of Science and Technology, Seoul, Korea.
authors.forename: Hoyoung
authors.initials: H
authors.lastname: Park
authors.affiliation: Center for Nanoparticle Research, Institute for Basic Science, Seoul, Korea ; School of Chemical and Biological Engineering, Seoul National University, Seoul, Korea.
authors.forename: Taeho
authors.initials: T
authors.lastname: Kim
authors.affiliation: Department of Bionanotechnology, Hanyang University, Seoul Campus, Seoul, Korea ; Department of Bionanoengineering, Hanyang University, ERICA Campus, Ansan, Korea.
authors.forename: Yoon
authors.initials: Y
authors.lastname: Jeong
authors.affiliation: Center for Nanoparticle Research, Institute for Basic Science, Seoul, Korea ; School of Chemical and Biological Engineering, Seoul National University, Seoul, Korea.
authors.forename: Myoung Hwan
authors.initials: MH
authors.lastname: Oh
authors.affiliation: Center for Nanoparticle Research, Institute for Basic Science, Seoul, Korea ; School of Chemical and Biological Engineering, Seoul National University, Seoul, Korea.
authors.forename: Taeghwan
authors.initials: T
authors.lastname: Hyeon
authors.affiliation: Department of Radiology and Radiological Health, Johns Hopkins University School of Medicine, Baltimore, MD, USA ; Institute for Cell Engineering, Baltimore, MD, USA.
authors.forename: Assaf A
authors.initials: AA
authors.lastname: Gilad
authors.affiliation: Center for Biomaterials, Biomedical Research Institute, Korea Institute of Science and Technology, Seoul, Korea.
authors.forename: Kwan Hyi
authors.initials: KH
authors.lastname: Lee
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry* / Q000494:pharmacology; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D003094:Collagen / Q000737:chemistry* / Q000494:pharmacology; D003692:Delayed-Action Preparations; D005293:Ferritins / Q000737:chemistry; D005347:Fibroblasts / Q000166:cytology; D005456:Fluorescent Dyes / Q000737:chemistry; D005881:Gingiva / Q000166:cytology / Q000187:drug effects*; D006046:Gold / Q000737:chemistry; D006801:Humans; D020100:Hydrogels / Q000737:chemistry* / Q000494:pharmacology; D008422:Materials Testing; D008954:Models, Biological; D010087:Oxides / Q000737:chemistry; D051381:Rats; D044968:Regenerative Medicine; D013635:Tantalum / Q000737:chemistry
chemical_list: D001672:Biocompatible Materials; D003692:Delayed-Action Preparations; D005456:Fluorescent Dyes; D020100:Hydrogels; D010087:Oxides; D013635:Tantalum; D006046:Gold; D003094:Collagen; D005293:Ferritins; C078151:tantalum oxide
keywords: biomolecules; engineered collagen hydrogels; growth; human gingival cells; imaging agents; sustained release