title: Stem cell augmented mesh materials: an in vitro and in vivo study.
abstract: OBJECTIVE
  To test in vitro and in vivo the capability of mesh materials to act as scaffolds for rat-derived mesenchymal stem cells (rMSCs) and to compare inflammatory response and collagen characteristics of implant materials, either seeded or not with rMSCs.
  
  
  METHODS
  rMSCs isolated from rat bone marrow were seeded and cultured in vitro on four different implant materials. Implants showing the best rMSC proliferation rate were selected for the in vivo experiment. Forty-eight adult female Sprague-Dawley rats were randomly divided into two treatment groups. The implant of interest-either seeded or not with rMSCs-was laid and fixed over the muscular abdominal wall. Main outcome measures were: in vitro, proliferation of rMSCs on selected materials; in vivo, the occurrence of topical complications, the evaluation of systemic and local inflammatory response and examination of the biomechanical properties of explants.
  
  
  RESULTS
  Surgisis and Pelvitex displayed the best cell growth in vitro. At 90 days in the rat model, rMSCs were related to a lower count of neutrophil cells for Pelvitex and a greater organisation and collagen amount for Surgisis. At 7 days Surgisis samples seeded with rMSCs displayed higher breaking force and stiffness.
  
  
  CONCLUSIONS
  The presence of rMSCs reduced the systemic inflammatory response on synthetic implants and improved collagen characteristics at the interface between biological grafts and native tissues. rMSCs enhanced the stripping force on biological explants.
authors.affiliation: Department of Obstetrics and Gynaecology, San Gerardo Hospital, University of Milano-Bicocca, Monza, Italy.
authors.forename: Federico
authors.initials: F
authors.lastname: Spelzini
authors.forename: Stefano
authors.initials: S
authors.lastname: Manodoro
authors.forename: Matteo
authors.initials: M
authors.lastname: Frigerio
authors.forename: Gabriella
authors.initials: G
authors.lastname: Nicolini
authors.forename: Daniele
authors.initials: D
authors.lastname: Maggioni
authors.forename: Elisabetta
authors.initials: E
authors.lastname: Donzelli
authors.forename: Lina
authors.initials: L
authors.lastname: Altomare
authors.forename: Silvia
authors.initials: S
authors.lastname: Farè
authors.forename: Fanny
authors.initials: F
authors.lastname: Veneziano
authors.forename: Federica
authors.initials: F
authors.lastname: Avezza
authors.forename: Giovanni
authors.initials: G
authors.lastname: Tredici
authors.forename: Rodolfo
authors.initials: R
authors.lastname: Milani
mesh_terms: D062088:Acellular Dermis / Q000009:adverse effects; D000818:Animals; D001672:Biocompatible Materials / Q000009:adverse effects; D049109:Cell Proliferation; D002478:Cells, Cultured; D003094:Collagen / Q000009:adverse effects / Q000378:metabolism / Q000648:ultrastructure; D004548:Elasticity; D005260:Female; D007249:Inflammation / Q000209:etiology; D007958:Leukocyte Count; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000502:physiology*; D009504:Neutrophils; D011126:Polypropylenes / Q000009:adverse effects; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D013526:Surgical Mesh / Q000009:adverse effects; D013718:Tensile Strength; D054457:Tissue Scaffolds / Q000009:adverse effects
chemical_list: D001672:Biocompatible Materials; D011126:Polypropylenes; C588278:surgisis; D003094:Collagen