title: [Effect on T cell subsets and secretion of IFN-γ and IL-17 after exposure to ⁶⁰Co lethally total body irradiation in mice].
abstract: OBJECTIVE
  To investigate the effects of letherally total body irradiation (TBI) on distribution of T-lymphocyte subtypes and their cytokine expression.
  
  
  METHODS
  The BALB/c mice were divided randomly into ⁶⁰Co gamma rays TBI group and control group. Mice were sacrificed 7 days after irradiation. The lymphocytes in spleens, mesenteric lymphonodes, livers and bone marrow were collected and counted. Changes of CD4(+) T and CD8(+) T cell subsets as well as the expressions of IFN-γ and IL-17 were analyzed by flow cytometry.
  
  
  RESULTS
  (1)Compared with control group, the total number of lymphocytes in marrow, spleen, lymph node and liver distinctively decreased in TBI group [(5.34±1.14)×10⁵ vs (3.08±1.13)×10⁷, (2.10±0.54)×10⁵ vs (2.71±0.83)×10⁷, (5.89±1.07)×10⁵ vs (7.92±1.15)×10⁷ and (3.45±1.01)×10⁵ vs (7.44±0.79)×10⁶, respectively, and the significant differences were observed between two groups in each organ (P<0.05)]. (2)The level of IFN-γ produced by CD4(+) T in spleen, lymph node and liver elevated in TBI group compared to control group, which were (20.77±2.03)% vs (3.69±3.13)%, (6.28±0.46)% vs (1.11±0.17)%, (27.24±5.79)% vs (9.01±1.24)% respectively, the differences between two groups in each organ were significant (P<0.05). (3)Percentages of IFN-γ(+)CD8(+) T in spleen, lymph node and liver in TBI group increased compared to control group [(52.40±9.26)% vs (43.06±1.04)%, (33.56±5.02)% vs (21.83±4.22)%, and (44.27±8.97)% vs (19.32±3.11)%, respectively, and the differences between two groups in each tissue were significant (P<0.05)]. (4)However, IL-17A expressions in CD4(+) T and CD8(+) T cells from spleen and liver were lower than those in control group.
  
  
  CONCLUSIONS
  TBI induced the reduction of lymphocytes and the expansion of IFN-γ producing Th1 and Tc1 effector cells in mice.
authors.affiliation: Department of Hematology, the Affiliated Hospital of Xuzhou Medical College; Transplantation Immunology Laboratory of Xuzhou Medical College, Xuzhou　221002, China.
authors.forename: Dongmei
authors.initials: D
authors.lastname: Zhao
authors.affiliation: Department of Hematology, the Affiliated Hospital of Xuzhou Medical College; Transplantation Immunology Laboratory of Xuzhou Medical College, Xuzhou　221002, China.
authors.forename: Lingling
authors.initials: L
authors.lastname: Yin
authors.affiliation: Department of Hematology, the Affiliated Hospital of Xuzhou Medical College; Transplantation Immunology Laboratory of Xuzhou Medical College, Xuzhou　221002, China.
authors.forename: Kailin
authors.initials: K
authors.lastname: Xu
authors.affiliation: Department of Hematology, the Affiliated Hospital of Xuzhou Medical College; Transplantation Immunology Laboratory of Xuzhou Medical College, Xuzhou　221002, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Zhao
mesh_terms: D000818:Animals; D007371:Interferon-gamma / Q000378:metabolism*; D020381:Interleukin-17 / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D016176:T-Lymphocyte Subsets / Q000276:immunology* / Q000378:metabolism; D014916:Whole-Body Irradiation
chemical_list: D020381:Interleukin-17; D007371:Interferon-gamma