title: Massively parallel sequencing of forensically relevant single nucleotide polymorphisms using TruSeq™ forensic amplicon.
abstract: The TruSeq™ Forensic Amplicon library preparation protocol, originally designed to attach sequencing adapters to chromatin-bound DNA for chromatin immunoprecipitation sequencing (TruSeq™ ChIP-Seq), was used here to attach adapters directly to amplicons containing markers of forensic interest. In this study, the TruSeq™ Forensic Amplicon library preparation protocol was used to detect 160 single nucleotide polymorphisms (SNPs), including human identification SNPs (iSNPs), ancestry, and phenotypic SNPs (apSNPs) in 12 reference samples. Results were compared with those generated by a second laboratory using the same technique, as well as to those generated by whole genome sequencing (WGS). The genotype calls made using the TruSeq™ Forensic Amplicon library preparation protocol were highly concordant. The protocol described herein represents an effective and relatively sensitive means of preparing amplified nuclear DNA for massively parallel sequencing (MPS).
authors.affiliation: Institute of Applied Genetics, Department of Molecular and Medical Genetics, University of North Texas Health Science Center, 3500 Camp Bowie Boulevard, Fort Worth, TX, 76107, USA.
authors.forename: David H
authors.initials: DH
authors.lastname: Warshauer
authors.forename: Carey P
authors.initials: CP
authors.lastname: Davis
authors.forename: Cydne
authors.initials: C
authors.lastname: Holt
authors.forename: Yonmee
authors.initials: Y
authors.lastname: Han
authors.forename: Paulina
authors.initials: P
authors.lastname: Walichiewicz
authors.forename: Tom
authors.initials: T
authors.lastname: Richardson
authors.forename: Kathryn
authors.initials: K
authors.lastname: Stephens
authors.forename: Anne
authors.initials: A
authors.lastname: Jager
authors.forename: Jonathan
authors.initials: J
authors.lastname: King
authors.forename: Bruce
authors.initials: B
authors.lastname: Budowle
mesh_terms: D017931:DNA Primers; D005819:Genetic Markers; D006579:Heterozygote; D059014:High-Throughput Nucleotide Sequencing; D006801:Humans; D020641:Polymorphism, Single Nucleotide; D060888:Real-Time Polymerase Chain Reaction; D017422:Sequence Analysis, DNA / Q000379:methods*
chemical_list: D017931:DNA Primers; D005819:Genetic Markers