title: A rapid LC-MS/MS method for quantification of CSUOH0901, a novel antitumor agent, in rat plasma.
abstract: CSUOH0901, a novel anticancer derivative of nimesulide, exhibits very promising anticancer activities in various cancer cell lines. In order to support further pharmacological and toxicological studies of this promising anticancer drug candidate, an LC-MS/MS method was developed and validated in accordance with the US Food and Drug Administration guidelines. The drug molecules were extracted from plasma samples by protein precipitation and then analyzed with LC-ESI-MS/MS. An excellent analyte separation was achieved using a phenomenex C18 column with a mobile phase of 90% methanol and 5 m m of ammonium formate. The validated linear dynamic range was between 0.5 and 100 ng/mL and the achieved correlation coefficient (r(2)) was >0.9996. The results of inter- and intra-day precision and accuracy were satisfactory, that is, <12% for accuracy and within ±5% for precision at a low and high quality control concentrations, respectively. In addition, the analyte and internal standard (JCC76) were found to be stable under the storage conditions at -20°C for about 2 months. Hence, the acquired results proved that the LC-ESI-MS/MS method developed is precise, accurate and selective for the quantification of CSUOH0901 in plasma, and can be used for pharmacokinetic studies.
authors.affiliation: Department of Chemistry, Cleveland State University, 2121 Euclid Avenue, Cleveland, OH, 44115, USA.
authors.forename: Ramakrishna R
authors.initials: RR
authors.lastname: Voggu
authors.forename: Ravali
authors.initials: R
authors.lastname: Alagandula
authors.forename: Xiang
authors.initials: X
authors.lastname: Zhou
authors.forename: Bin
authors.initials: B
authors.lastname: Su
authors.forename: Bo
authors.initials: B
authors.lastname: Zhong
authors.forename: Baochuan
authors.initials: B
authors.lastname: Guo
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000097:blood*; D052117:Benzodioxoles / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005260:Female; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D013449:Sulfonamides / Q000097:blood*; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D000970:Antineoplastic Agents; D052117:Benzodioxoles; D013449:Sulfonamides; C574650:benzo(1,3)dioxole-5-carboxylic acid (3-(2,5-dimethylbenzyloxy)-4-(methanesulfonylmethylamino)phenyl)amide
keywords: CSUOH0901; LC-MS/MS; nimesulide; protein precipitation; rat plasma