title: Studies on a suitable antibiotic therapy for treating swine brucellosis.
abstract: The aim of this work was developing effective treatments against Brucella suis biovar 2, responsible for swine brucellosis in Europe. MICs for antibiotics used classically in brucellosis and two new macrolides (tulathromycin and tildipirosin) were determined for 33 B. suis biovar 2 field and B. suis reference strains. MIC90 values ranged from 0.01 to 0.25 μg/mL. The best candidates, given alone or combined, were then evaluated in mice. Ten groups (n = 7) of BALB/c mice were inoculated (1 × 10(5) CFU/mouse) with a virulent B. suis biovar 2 field strain. All groups, excepting untreated control, were treated for 14 days with, respectively, doxycycline, dihydrostreptomycin, tulathromycin (one or two doses), or tildipirosin (one or two doses) given alone, and doxycycline combined with dihydrostreptomycin, tulathromycin, or tildipirosin. Combined tildipirosin treatment was the most effective, then selected for pig studies. Sixteen B. suis biovar 2 naturally infected sows were treated with oxytetracycline (20 mg/kg BW/daily) for 21 days. The half of these received also tildipirosin (4 mg/kg BW) in two doses with a 10-day interval. An extensive bacteriological study conducted ten days after ceasing treatments proved the efficacy of this combined oxytetracycline/tildipirosin treatment.
authors.affiliation: Unidad de Sanidad Animal, Centro de Investigación y Tecnología Agroalimentaria (CITA), Zaragoza, Spain.
authors.forename: L
authors.initials: L
authors.lastname: Dieste-Pérez
authors.affiliation: Departamento de Producción Animal, Universitat de Lleida, Lleida, Spain.
authors.forename: L
authors.initials: L
authors.lastname: Fraile
authors.affiliation: Unidad de Sanidad Animal, Centro de Investigación y Tecnología Agroalimentaria (CITA), Zaragoza, Spain.
authors.forename: M J
authors.initials: MJ
authors.lastname: de Miguel
authors.affiliation: Facultad de Veterinaria, Departamento de Patología Animal de la, Universidad de Zaragoza, Zaragoza, Spain.
authors.forename: M
authors.initials: M
authors.lastname: Barberán
authors.affiliation: Unidad de Sanidad Animal, Centro de Investigación y Tecnología Agroalimentaria (CITA), Zaragoza, Spain.
authors.forename: J M
authors.initials: JM
authors.lastname: Blasco
authors.affiliation: Unidad de Sanidad Animal, Centro de Investigación y Tecnología Agroalimentaria (CITA), Zaragoza, Spain.
authors.forename: P M
authors.initials: PM
authors.lastname: Muñoz
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000627:therapeutic use*; D041021:Brucella suis; D002006:Brucellosis / Q000188:drug therapy / Q000382:microbiology / Q000662:veterinary*; D004187:Disaccharides / Q000627:therapeutic use*; D004359:Drug Therapy, Combination; D005260:Female; D006571:Heterocyclic Compounds / Q000627:therapeutic use*; D051379:Mice; D008807:Mice, Inbred BALB C; D008826:Microbial Sensitivity Tests; D010118:Oxytetracycline / Q000008:administration & dosage / Q000627:therapeutic use; D013552:Swine; D013553:Swine Diseases / Q000188:drug therapy* / Q000382:microbiology; D015645:Tylosin / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000627:therapeutic use; D018942:Macrolides
chemical_list: D000900:Anti-Bacterial Agents; D004187:Disaccharides; D006571:Heterocyclic Compounds; D010118:Oxytetracycline; D015645:Tylosin; C576258:20,23-dipiperidinyl-mycaminosyl-tylonolide; C485204:tulathromycin; D018942:Macrolides