title: MicroRNA-9 controls dendritic development by targeting REST.
abstract: MicroRNAs (miRNAs) are conserved noncoding RNAs that function as posttranscriptional regulators of gene expression. miR-9 is one of the most abundant miRNAs in the brain. Although the function of miR-9 has been well characterized in neural progenitors, its role in dendritic and synaptic development remains largely unknown. In order to target miR-9 in vivo, we developed a transgenic miRNA sponge mouse line allowing conditional inactivation of the miR-9 family in a spatio-temporal-controlled manner. Using this novel approach, we found that miR-9 controls dendritic growth and synaptic transmission in vivo. Furthermore, we demonstrate that miR-9-mediated downregulation of the transcriptional repressor REST is essential for proper dendritic growth.
authors.affiliation: Department of Molecular Neurobiology, Max Planck Institute of Psychiatry, Munich, Germany.
authors.forename: Sebastian A
authors.initials: SA
authors.lastname: Giusti
authors.affiliation: Department of Molecular Neurobiology, Max Planck Institute of Psychiatry, Munich, Germany.
authors.forename: Annette M
authors.initials: AM
authors.lastname: Vogl
authors.affiliation: Department of Molecular Neurobiology, Max Planck Institute of Psychiatry, Munich, Germany.
authors.forename: Marisa M
authors.initials: MM
authors.lastname: Brockmann
authors.affiliation: Department of Molecular Neurobiology, Instituto de Investigación en Biomedicina de Buenos Aires (IBioBA)-CONICET-Partner Institute of the Max Planck Society, Buenos Aires, Argentina.
authors.forename: Claudia A
authors.initials: CA
authors.lastname: Vercelli
authors.affiliation: Department of Neurobiology of Stress and Neurogenetics, Max Planck Institute of Psychiatry, Munich, Germany.
authors.forename: Martin L
authors.initials: ML
authors.lastname: Rein
authors.affiliation: Institute of Developmental Genetics, Helmholtz Zentrum München, Neuherberg, Germany.
authors.forename: Dietrich
authors.initials: D
authors.lastname: Trümbach
authors.affiliation: Institute of Developmental Genetics, Helmholtz Zentrum München, Neuherberg, Germany.
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Wurst
authors.affiliation: Department of Biochemistry, University of Utah School of Medicine, Salt Lake City, United States.
authors.forename: Demian
authors.initials: D
authors.lastname: Cazalla
authors.affiliation: Institute of Physiology, University of Bonn, Bonn, Germany.
authors.forename: Valentin
authors.initials: V
authors.lastname: Stein
authors.affiliation: Department of Neurobiology of Stress and Neurogenetics, Max Planck Institute of Psychiatry, Munich, Germany.
authors.forename: Jan M
authors.initials: JM
authors.lastname: Deussing
authors.affiliation: Department of Molecular Neurobiology, Max Planck Institute of Psychiatry, Munich, Germany.
authors.forename: Damian
authors.initials: D
authors.lastname: Refojo
mesh_terms: D000375:Aging / Q000378:metabolism; D000818:Animals; D001921:Brain / Q000378:metabolism; D002478:Cells, Cultured; D003712:Dendrites / Q000378:metabolism*; D017930:Genes, Reporter; D057809:HEK293 Cells; D006801:Humans; D019426:Integrases / Q000378:metabolism; D008822:Mice, Transgenic; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D064231:Nestin / Q000378:metabolism; D009474:Neurons / Q000378:metabolism; D012097:Repressor Proteins / Q000378:metabolism*; D009435:Synaptic Transmission; D000099229:RE1-Silencing Transcription Factor
chemical_list: D019426:Integrases; D035683:MicroRNAs; D064231:Nestin; D012097:Repressor Proteins; D000099229:RE1-Silencing Transcription Factor; C551804:MIRN9 microRNA, mouse; C045073:Cre recombinase
keywords: REST; dendrite development; miR-9; miR-9 reporter; miR-9 sponge; mouse; neuroscience