title: Embryo developmental potential of microsurgically corrected human three-pronuclear zygotes.
abstract: We explored the embryo development potential of human three-pronuclear (3PN) zygotes reduced to two-pronuclear (2PN) zygotes (3 → 2PN zygotes) by micropuncture. In this study, there were three groups, the 3 → 2PN group (338 zygotes), the non-corrected 3PN group (381 zygotes), and the normal 2PN group (359 zygotes). The first cleavage mode (2-cell cleavage or 3-cell cleavage), 6-8 cell embryogenesis rate, high-quality embryogenesis rate and Day 5/Day 6 blastulation rate were compared between the three groups. The success rate of enucleation was 92.9%. The 2-cell cleavage rate was significantly higher in the 3 → 2PN group (74.3%) than in the 3PN group (36.4%) (P < 0.05), but had no statistical difference compared with the 2PN group (86.0%) (P > 0.05). The 6-8 cell embryogenesis rate was significantly higher in the 3 → 2PN group (91.1%) as compared to the 2PN group (85.6%) (P < 0.05), but had no statistical difference compared with the 3PN group (95.0%) (P > 0.05). Total blastulation rate was significantly higher in the 2PN group (58.8%) as compared to the 3PN group (21.5%) (P < 0.01), and in the 3 → 2PN group as compared to the 3PN group (5.6%) (P < 0.01). Also D5 blastulation rate was significantly higher in the 2PN group (53.7%) as compared to the 3 → 2PN group (8.9%) (P < 0.01), and in the 3 → 2PN group as compared to the 3PN group (1.9%) (P < 0.01). In 3 → 2PN zygotes, the first cleavage mode is mainly 2 cells which is significantly higher than that in 3PN zygotes. Compared with 3PN zygotes, the embryo developmental potential of 3 → 2PN zygotes is improved, but still is lower than that in 2PN zygotes.
authors.affiliation: Reproductive Medical Center, First Affiliated Hospital of Zhengzhou University , Zhengzhou , China.
authors.forename: Hai-xia
authors.initials: HX
authors.lastname: Jin
authors.forename: Shan-jun
authors.initials: SJ
authors.lastname: Dai
authors.forename: Wen-yan
authors.initials: WY
authors.lastname: Song
authors.forename: Gui-dong
authors.initials: GD
authors.lastname: Yao
authors.forename: Sen-lin
authors.initials: SL
authors.lastname: Shi
authors.forename: Ying-pu
authors.initials: YP
authors.lastname: Sun
mesh_terms: D000328:Adult; D001755:Blastocyst / Q000648:ultrastructure; D002970:Cleavage Stage, Ovum; D004622:Embryo, Mammalian / Q000601:surgery*; D047108:Embryonic Development / Q000502:physiology*; D005260:Female; D005306:Fertilization; D005307:Fertilization in Vitro; D006801:Humans; D007246:Infertility; D007621:Karyotyping; D008297:Male; D046228:Microarray Analysis; D008866:Microsurgery / Q000379:methods*; D010062:Ovulation Induction; D020641:Polymorphism, Single Nucleotide
keywords: Blastula; micropuncture; polyspermic fertilization; three-pronuclear zygotes