title: Influence of recombinant lentiviral vector encoding miR-15a/16-1 in biological features of human nasopharyngeal carcinoma CNE-2Z cells.
abstract: MicroRNAs can function as tumor suppressor miRNAs. Bcl-2 is an antiapoptotic gene overexpressed in many tumors, including nasopharyngeal carcinoma (NPC). It is reported that microRNA-15a (miR-15a) and microRNA-16-1 (miR-16-1) could act as bcl-2 inhibitors. To investigate their effects on NPC, the authors used recombinant lentiviral vector to upregulate the expression of miR-15a/16-1 in NPC CNE-2Z cells. The authors divided cells into the control group, transfection group, radiotherapy group, and transfection-radiotherapy group. In this experiment, reverse transcription-quantitative polymerase chain reaction was used to detect the expression of miR-15a/16-1 and bcl-2 mRNA. Cell proliferation was analyzed by MTT assay. Flow cytometry was used to measure cell apoptosis. Radiosensitivity was measured using colony-forming experiment. The protein expression of bcl-2 was measured by western blot, the activation levels of caspase were detected by a spectrophotometric method. After transfection, cell proliferation was inhibited, while the apoptosis rate and radiosensitivity were increased. In addition, the activation of caspase-2 and caspase-3 was aggrandized correspondingly. Although the expression levels of bcl-2 mRNA in each group had no difference, the protein expression of bcl-2 was downregulated. These results suggested that miR-15a/16-1 could inhibit cell proliferation and increase the apoptosis and radiosensitivity of CNE-2 cells, by regulating the bcl-2 gene at post-transcriptional level and by increasing the activation of caspase-2 and caspase-3.
authors.affiliation: 1 Department of Otorhinolaryngology, the First Affiliated Hospital of Wenzhou Medical College , Wenzhou, Zhejiang, China .
authors.forename: Chunhong
authors.initials: C
authors.lastname: Zhang
authors.forename: Xiaobi
authors.initials: X
authors.lastname: Fang
authors.forename: Wenfeng
authors.initials: W
authors.lastname: Li
authors.forename: Qingyuan
authors.initials: Q
authors.lastname: Shi
authors.forename: Liping
authors.initials: L
authors.lastname: Wu
authors.forename: Xiaoyun
authors.initials: X
authors.lastname: Chen
authors.forename: Zhenxiao
authors.initials: Z
authors.lastname: Huang
authors.forename: Peng
authors.initials: P
authors.lastname: Wu
authors.forename: Zhenzhen
authors.initials: Z
authors.lastname: Wang
authors.forename: Zhisu
authors.initials: Z
authors.lastname: Liao
mesh_terms: D017209:Apoptosis / Q000235:genetics; D002277:Carcinoma; D053143:Caspase 2 / Q000235:genetics; D053148:Caspase 3 / Q000235:genetics; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics; D003546:Cysteine Endopeptidases / Q000235:genetics; D005822:Genetic Vectors / Q000235:genetics*; D006801:Humans; D016086:Lentivirus / Q000235:genetics*; D035683:MicroRNAs / Q000235:genetics*; D000077274:Nasopharyngeal Carcinoma; D009303:Nasopharyngeal Neoplasms / Q000235:genetics*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D011836:Radiation Tolerance / Q000235:genetics; D014162:Transfection / Q000379:methods
chemical_list: C505361:MIRN15 microRNA, human; C505362:MIRN16 microRNA, human; D035683:MicroRNAs; D019253:Proto-Oncogene Proteins c-bcl-2; D012333:RNA, Messenger; C490360:CASP2 protein, human; D053143:Caspase 2; D053148:Caspase 3; D003546:Cysteine Endopeptidases
keywords: apoptosis; bcl-2; microRNAs; nasopharyngeal carcinoma; radiosensitivity