title: The aggregation of aβ42 induced by nano copper and the antagonistic action of polysaccharides.
abstract: The toxic effect of Aβ42 induced by copper nanoparticle (Cu NPs) was studied by atomic force microscopy (AFM), circular dichroism (CD) spectroscopy, and Thioflavin T (ThT) fluorescence technique. Five hundred nanometers of copper nanoparticle capped with polyvinylpyrrolidone (PVP) was used to evaluate the aggregation and fibrils of Aβ42. The morphologies of Aβ42 incubated in the presence of Cu NPs changed gradually. The aggregation and fibrils were observed in AFM images. However, in the presence of polysaccharides, the Cu NPs-induced fibrillation of Aβ42 was inhibited. Interestingly, the formed Cu NPs-polysaccharides complexes can even remodel the preformed Aβ42 fibrils into the low neurotoxic amorphous aggregates, which were maybe ascribed to the higher affinity of polysaccharides for Aβ42 than Cu NPs. Besides, it was found that the binding constant of Cu NPs to Aβ42 is smaller than that of polysaccharides. The relationship among polysaccharides, copper nanoparticle, and Aβ42 morphologies and its neurotoxicity were discussed, and the binding force was analyzed.
authors.affiliation: Department of Biological Sciences and BioTechnology, Minnan Normal University, Zhangzhou, 363000, China, wangwei0596@163.com.
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.forename: Guoguang
authors.initials: G
authors.lastname: Zhang
authors.forename: Jinmei
authors.initials: J
authors.lastname: Zou
mesh_terms: D016229:Amyloid beta-Peptides / Q000737:chemistry*; D052160:Benzothiazoles; D002942:Circular Dichroism; D003300:Copper / Q000737:chemistry*; D005453:Fluorescence; D007700:Kinetics; D018625:Microscopy, Atomic Force; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D010446:Peptide Fragments / Q000737:chemistry*; D011134:Polysaccharides / Q000494:pharmacology*; D066329:Protein Aggregates; D017433:Protein Structure, Secondary; D013844:Thiazoles / Q000737:chemistry; D014961:X-Ray Diffraction
chemical_list: D016229:Amyloid beta-Peptides; D052160:Benzothiazoles; D010446:Peptide Fragments; D011134:Polysaccharides; D066329:Protein Aggregates; D013844:Thiazoles; C075222:amyloid beta-protein (1-42); C009462:thioflavin T; D003300:Copper