title: Noble-Collip Drum Trauma Induces Disseminated Intravascular Coagulation But Not Acute Coagulopathy of Trauma-Shock.
abstract: BACKGROUND
  There are two opposing possibilities for the main pathogenesis of trauma-induced coagulopathy: an acute coagulopathy of trauma shock and disseminated intravascular coagulation with the fibrinolytic phenotype.
  
  
  OBJECTIVE
  The objective of this study was to clarify the main pathogenesis of trauma-induced coagulopathy using a rat model of Noble-Collip drum trauma.
  
  
  METHODS
  Eighteen rats were divided into the control, trauma 0, and trauma 30 groups. The trauma 0 and 30 groups were exposed to Noble-Collip drum trauma. Blood samples were drawn without, immediately after, and 30 min after Noble-Collip drum trauma in the control, trauma 0, and trauma 30 groups, respectively. Coagulation and fibrinolysis markers were measured. Thrombin generation was assessed according to a calibrated automated thrombogram.
  
  
  RESULTS
  Spontaneous thrombin bursts resulting from circulating procoagulants were observed in the nonstimulated thrombin generation assay immediately after trauma. Soluble fibrin levels (a marker of thrombin generation in the systemic circulation) were 50-fold greater in the trauma groups than in the control group. The resultant coagulation activation consumed platelets, coagulation factors, and antithrombin. Endogenous thrombin potential and factor II ratio were significantly negatively correlated with antithrombin levels, suggesting insufficient control of thrombin generation by antithrombin. High levels of active tissue-type plasminogen activator induced hyperfibrin(ogen)olysis. Soluble thrombomodulin increased significantly. However, activated protein C levels did not change.
  
  
  CONCLUSIONS
  The systemic thrombin generation accelerated by insufficient antithrombin control leads to the consumption of platelets and coagulation factors associated with hyperfibrin(ogen)olysis. These changes are collectively termed disseminated intravascular coagulation with the fibrinolytic phenotype.
authors.affiliation: *Division of Acute and Critical Care Medicine, Department of Anesthesiology and Critical Care Medicine, Hokkaido University Graduate School of Medicine, Sapporo; and †Department of Internal Medicine, School of Dentistry, Health Sciences University of Hokkaido, Ishikari-gun, Japan.
authors.forename: Mineji
authors.initials: M
authors.lastname: Hayakawa
authors.forename: Satoshi
authors.initials: S
authors.lastname: Gando
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Ono
authors.forename: Takeshi
authors.initials: T
authors.lastname: Wada
authors.forename: Yuichiro
authors.initials: Y
authors.lastname: Yanagida
authors.forename: Atsushi
authors.initials: A
authors.lastname: Sawamura
authors.forename: Masahiro
authors.initials: M
authors.lastname: Ieko
mesh_terms: D000818:Animals; D001778:Blood Coagulation Disorders / Q000097:blood / Q000209:etiology*; D004195:Disease Models, Animal; D004211:Disseminated Intravascular Coagulation / Q000097:blood / Q000209:etiology*; D005338:Fibrin Fibrinogen Degradation Products / Q000378:metabolism; D005342:Fibrinolysis; D008297:Male; D010641:Phenotype; D051381:Rats; D017208:Rats, Wistar; D012774:Shock, Traumatic / Q000097:blood / Q000150:complications*; D013917:Thrombin / Q000096:biosynthesis; D010959:Tissue Plasminogen Activator / Q000097:blood
chemical_list: D005338:Fibrin Fibrinogen Degradation Products; D013917:Thrombin; D010959:Tissue Plasminogen Activator