title: Immobilization of Streptomyces thermotolerans 11432 on polyurethane foam to improve production of acetylisovaleryltylosin.
abstract: In this study, polyurethane foam (PUF) was chemically treated to immobilize Streptomyces thermotolerans 11432 for semi-continuous production of acetylisovaleryltylosin (AIV). Based on experimental results, positive cross-linked PUF (PCPUF) was selected as the most effective carrier according to immobilized cell mass. The effect of adsorption time on immobilized mass was investigated. AIV concentration (33.54 mg/l) in batch fermentations with immobilized cells was higher than with free cells (20.34 mg/l). In repeated batch fermentations with immobilized S. thermotolerans 11432 using PCPUF cubes, high AIV concentrations and conversion rates were attained, ranging from 25.56 to 34.37 mg/l and 79.93 to 86.31 %, respectively. Significantly, this method provides a feasible strategy for efficient AIV production and offers the potential for large-scale production.
authors.affiliation: Key Laboratory of Systems Bioengineering, Ministry of Education, School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072, China.
authors.forename: Hongji
authors.initials: H
authors.lastname: Zhu
authors.forename: Weihua
authors.initials: W
authors.lastname: Wang
authors.forename: Jiaheng
authors.initials: J
authors.lastname: Liu
authors.forename: Qinggele
authors.initials: Q
authors.lastname: Caiyin
authors.forename: Jianjun
authors.initials: J
authors.lastname: Qiao
mesh_terms: D000327:Adsorption; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D018914:Cells, Immobilized; D003001:Cloning, Molecular; D005285:Fermentation; D011140:Polyurethanes / Q000737:chemistry*; D013302:Streptomyces / Q000378:metabolism*; D015645:Tylosin / Q000031:analogs & derivatives* / Q000096:biosynthesis
chemical_list: D001426:Bacterial Proteins; D011140:Polyurethanes; C085543:acyB2 protein, Streptomyces thermotolerans; C031783:3-acetyl-4''-isovaleryltylosin; C028279:polyurethane foam; D015645:Tylosin