title: Reactions of chicken sera to recombinant Campylobacter jejuni flagellar proteins.
abstract: Campylobacter jejuni is a Gram-negative spiral rod bacterium and is the leading but underreported bacterial food-borne pathogen that causes human campylobacteriosis worldwide. Raw or undercooked poultry products are regarded as a major source for human infection. C. jejuni flagella have been implicated in colonization and adhesion to the mucosal surface of chicken gastrointestinal tracts. Therefore, flagellar proteins would be the excellent targets for further investigation. In this report, we used the recombinant technology to generate a battery of C. jejuni flagellar proteins, which were purified by His tag affinity chromatography and determined antigenic profiles of these recombinant flagellar proteins using sera from chickens older than 6 weeks of age. The immunoblot results demonstrate that each chicken serum reacted to various numbers of recombinant flagellar proteins. Among these recombinant proteins, chicken sera reacted predominantly to the FlgE1, FlgK, FlhF, FliG and FliY proteins. These antibody screening results provide a rationale for further evaluation of these recombinant flagellar proteins as potential vaccines for chickens to improve food safety as well as investigation of host immune response to C. jejuni.
authors.affiliation: Poultry Microbiological Safety Research Unit, Richard B. Russell Agricultural Research Center, United States Department of Agriculture, Agricultural Research Service, 950 College Station Road, Athens, GA, 30605-2720, USA, hungyueh.yeh@ars.usda.gov.
authors.forename: Hung-Yueh
authors.initials: HY
authors.lastname: Yeh
authors.forename: Kelli L
authors.initials: KL
authors.lastname: Hiett
authors.forename: John E
authors.initials: JE
authors.lastname: Line
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood*; D000942:Antigens, Bacterial / Q000235:genetics / Q000276:immunology*; D016123:Campylobacter jejuni / Q000235:genetics / Q000276:immunology*; D002645:Chickens; D004926:Escherichia coli / Q000235:genetics; D005407:Flagella / Q000737:chemistry; D015151:Immunoblotting; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology*
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D011994:Recombinant Proteins