title: Differentiation of mint (Mentha haplocalyx Briq.) from different regions in China using gas and liquid chromatography.
abstract: In this study, complex substances such as Mint (Mentha haplocalyx Briq.) samples from different growing regions in China were analyzed for phenolic compounds by high-performance liquid chromatography with diode array detection and for the volatile aroma compounds by gas chromatography with mass spectrometry. Chemometrics methods, e.g. principal component analysis, back-propagation artificial neural networks, and partial least squares discriminant analysis, were applied to resolve complex chromatographic profiles of Mint samples. A total of 49 aroma components and 23 phenolic compounds were identified in 79 Mint samples. Principal component analysis score plots from gas chromatography with mass spectrometry and high-performance liquid chromatography with diode array detection data sets showed a clear distinction among Mint from three different regions in China. Classification results showed that satisfactory performance of prediction ability for back-propagation artificial neural networks and partial least squares discriminant analysis. The major compounds that contributed to the discrimination were chlorogenic acid, unknown 3, kaempherol 7-O-rutinoside, salvianolic acid L, hesperidin, diosmetin, unknown 6 and pebrellin in Mint according to regression coefficients of the partial least squares discriminant analysis model. This study indicated that the proposed strategy could provide a simple and rapid technique to distinguish clearly complex profiles from samples such as Mint.
authors.affiliation: State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang, China; Spice and Beverage Research Institute, Chinese Academy of Tropical Agricultural Sciences (CATAS), Wanning, China.
authors.forename: Wenjiang
authors.initials: W
authors.lastname: Dong
authors.forename: Yongnian
authors.initials: Y
authors.lastname: Ni
authors.forename: Serge
authors.initials: S
authors.lastname: Kokot
mesh_terms: D002681:China; D002849:Chromatography, Gas; D002851:Chromatography, High Pressure Liquid; D016002:Discriminant Analysis; D016018:Least-Squares Analysis; D027541:Mentha / Q000737:chemistry*; D010636:Phenols / Q000032:analysis*; D025341:Principal Component Analysis
chemical_list: D010636:Phenols
keywords: Chemometrics; Gas chromatography; Geographical origins; High-performance liquid chromatography; Mint