title: Automated chemiluminescence immunoassay for a nonionic surfactant using a recycled spinning-pausing controlled washing procedure on a compact disc-type microfluidic platform.
abstract: A fully automated and integrated chemiluminescence immunoassay, carried out on a compact disc (CD)-type microfluidic platform, for the detection of alkylphenol polyethoxylates (APnEOs) is described. The pattern of the CD-type microchip was designed so as to permit the sequential solution delivery of the sample solution, the washing solution and the luminol solution, which are required in the chemiluminescence immunoassay process, along with a designed rotation program for spinning the CD-type microchip. The procedure for flowing the washing solution, the volume of which was limited on the CD-type microchip, was optimized by using a recycled spinning-pausing rotation program to overcome the non-specific adsorption of the horseradish peroxidase labeled APnEOs at the detection area. The detection limit of the immunoassay is about 10 ppb.
authors.affiliation: Department of Applied Chemistry, Graduate School of Engineering, Kyushu University, Fukuoka, Japan.
authors.forename: Shuai
authors.initials: S
authors.lastname: Guo
authors.affiliation: Department of Applied Chemistry, Graduate School of Engineering, Kyushu University, Fukuoka, Japan.
authors.forename: Ryoichi
authors.initials: R
authors.lastname: Ishimatsu
authors.affiliation: Department of Applied Chemistry, Graduate School of Engineering, Kyushu University, Fukuoka, Japan.
authors.forename: Koji
authors.initials: K
authors.lastname: Nakano
authors.affiliation: Department of Applied Chemistry, Graduate School of Engineering, Kyushu University, Fukuoka, Japan. Electronic address: imato@cstf.kyush-u.ac.jp.
authors.forename: Toshihiko
authors.initials: T
authors.lastname: Imato
mesh_terms: D004867:Equipment Design; D017022:Flow Injection Analysis / Q000295:instrumentation; D007118:Immunoassay / Q000295:instrumentation*; D008163:Luminescent Measurements / Q000295:instrumentation*; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D013501:Surface-Active Agents / Q000032:analysis*
chemical_list: D013501:Surface-Active Agents
keywords: Chemiluminescence immunoassay; Compact disc (CD)-type microfluidic platform; Nonionic surfactant; Recycled spinning-pausing washing procedure