title: The formin FMNL3 assembles plasma membrane protrusions that participate in cell-cell adhesion.
abstract: FMNL3 is a vertebrate-specific formin protein previously shown to play a role in angiogenesis and cell migration. Here we define the cellular localization of endogenous FMNL3, the dynamics of GFP-tagged FMNL3 during cell migration, and the effects of FMNL3 suppression in mammalian culture cells. The majority of FMNL3 localizes in a punctate pattern, with >95% of these puncta being indistinguishable from the plasma membrane by fluorescence microscopy. A small number of dynamic cytoplasmic FMNL3 patches also exist, which enrich near cell-cell contact sites and fuse with the plasma membrane at these sites. These cytoplasmic puncta appear to be part of larger membranes of endocytic origin. On the plasma membrane, FMNL3 enriches particularly in filopodia and membrane ruffles and at nascent cell-cell adhesions. FMNL3-containing filopodia occur both at the cell-substratum interface and at cell-cell contacts, with the latter being 10-fold more stable. FMNL3 suppression by siRNA has two major effects: decrease in filopodia and compromised cell-cell adhesion in cells migrating as a sheet. Overall our results suggest that FMNL3 functions in assembly of actin-based protrusions that are specialized for cell-cell adhesion.
authors.affiliation: Department of Biochemistry, Geisel School of Medicine at Dartmouth, Hanover, NH 03755.
authors.forename: Timothy J
authors.initials: TJ
authors.lastname: Gauvin
authors.affiliation: Department of Biochemistry, Geisel School of Medicine at Dartmouth, Hanover, NH 03755.
authors.forename: Lorna E
authors.initials: LE
authors.lastname: Young
authors.affiliation: Department of Biochemistry, Geisel School of Medicine at Dartmouth, Hanover, NH 03755 henry.higgs@dartmouth.edu.
authors.forename: Henry N
authors.initials: HN
authors.lastname: Higgs
mesh_terms: D000199:Actins / Q000378:metabolism; D000818:Animals; D002448:Cell Adhesion; D002462:Cell Membrane / Q000502:physiology*; D000080865:Formins; D006801:Humans; D051379:Mice; D011506:Proteins / Q000378:metabolism / Q000502:physiology*; D011554:Pseudopodia / Q000378:metabolism / Q000502:physiology*; D034741:RNA, Small Interfering
chemical_list: D000199:Actins; C476209:FMNL3 protein, human; C570808:FMNL3 protein, mouse; D000080865:Formins; D011506:Proteins; D034741:RNA, Small Interfering