title: Helicobacter pylori vacA and cagA genotype diversity and interferon gamma expression in patients with chronic gastritis and patients with gastric cancer.
abstract: BACKGROUND
  Helicobacter pylori (H. pylori) is the main risk factor for the development of chronic gastritis, gastric ulcer, and gastric cancer. In H. pylori-infected individuals, the clinical result is dependent on various factors, among which are bacterial components, the immune response, and environmental influence.
  
  
  OBJECTIVE
  To compare IFN-γ expression with the H. pylori vacA and cagA genotypes in patients with chronic gastritis and patients with gastric cancer.
  
  
  METHODS
  Ninety-five patients diagnosed with chronic gastritis and 20 with gastric cancer were included in the study. Three gastric biopsies were taken; one was used for the molecular detection and genotyping of H. pylori; another was fixed in absolute alcohol and histologic sections were made for determining IFN-γ expression through immunohistochemistry.
  
  
  RESULTS
  No differences were found in the cells that expressed IFN-γ between the patients with chronic gastritis (median percentage of positive cells: 82.6% in patients without H. pylori and 82% in infected persons) and those with gastric cancer (70.5% in H. pylori-negative patients and 78.5% in infected persons). IFN-γ expression was 69% in chronic gastritis patients infected with H. pylori vacAs2m2/cagA⁻ it was 86.5% in patients infected with H. pylori vacAs1m2/cagA⁻, 86.5% in vacAs1m1/cagA⁻, and 82% in vacAs1m1/cagA⁺. Similar data were found in the patients with gastric cancer.
  
  
  CONCLUSIONS
  IFN-γ expression varied depending on the H. pylori vacA and cagA genotype, but not in accordance with the presence of chronic gastritis or gastric cancer.
authors.forename: D N
authors.initials: DN
authors.lastname: Martínez-Carrillo
authors.forename: J
authors.initials: J
authors.lastname: Atrisco-Morales
authors.forename: R
authors.initials: R
authors.lastname: Hernández-Pando
authors.forename: S
authors.initials: S
authors.lastname: Reyes-Navarrete
authors.forename: R
authors.initials: R
authors.lastname: Betancourt-Linares
authors.forename: I
authors.initials: I
authors.lastname: Cruz-del Carmen
authors.forename: B
authors.initials: B
authors.lastname: Illades Aguiar
authors.forename: A
authors.initials: A
authors.lastname: Román-Román
authors.forename: G
authors.initials: G
authors.lastname: Fernández-Tilapa
mesh_terms: D000328:Adult; D000368:Aged; D002908:Chronic Disease; D005260:Female; D005756:Gastritis / Q000378:metabolism* / Q000382:microbiology*; D005798:Genes, Bacterial / Q000235:genetics; D014644:Genetic Variation; D005838:Genotype; D016481:Helicobacter Infections / Q000382:microbiology; D016480:Helicobacter pylori / Q000235:genetics*; D006801:Humans; D007371:Interferon-gamma / Q000096:biosynthesis*; D008297:Male; D008875:Middle Aged; D013274:Stomach Neoplasms / Q000378:metabolism* / Q000382:microbiology*
chemical_list: D007371:Interferon-gamma