title: Applications of hydrophilic interaction chromatography to amino acids, peptides, and proteins.
abstract: This review summarizes the recent advances in the analysis of amino acids, peptides, and proteins using hydrophilic interaction chromatography. Various reports demonstrate the successful analysis of amino acids under such conditions. However, a baseline resolution of the 20 natural amino acids has not yet been published and for this reason, there is often a need to use mass spectrometry for detection to further improve selectivity. Hydrophilic interaction chromatography is also recognized as a powerful technique for peptide analysis, and there are a lot of papers showing its applicability for proteomic applications (peptide mapping). It is expected that its use for peptide mapping will continue to grow in the future, particularly because this analytical strategy can be combined with reversed-phase liquid chromatography, in a two-dimensional setup, to reach very high resolving power. Finally, the interest in hydrophilic interaction chromatography for intact proteins analysis is less evident due to possible solubility issues and a lack of suitable hydrophilic interaction chromatography stationary phases. To date, it has been successfully employed only for the characterization of membrane proteins, histones, and the separation of glycosylated isoforms of an intact glycoprotein. From our point of view, the number of hydrophilic interaction chromatography columns compatible with intact proteins (higher upper temperature limit, large pore size, etc.) is still too limited.
authors.affiliation: School of Pharmaceutical Sciences, University of Geneva, University of Lausanne, Geneva, Switzerland.
authors.forename: Aurélie
authors.initials: A
authors.lastname: Periat
authors.forename: Ira S
authors.initials: IS
authors.lastname: Krull
authors.forename: Davy
authors.initials: D
authors.lastname: Guillarme
mesh_terms: D000596:Amino Acids / Q000032:analysis*; D002845:Chromatography; D057927:Hydrophobic and Hydrophilic Interactions; D010455:Peptides / Q000032:analysis*; D011506:Proteins / Q000032:analysis*
chemical_list: D000596:Amino Acids; D010455:Peptides; D011506:Proteins
keywords: Amino acids; Hydrophilic interaction chromatography; Peptides; Proteins