title: Endostatin level in cerebrospinal fluid of patients with Alzheimer's disease.
abstract: The aim of this study was to measure the level of endostatin, a fragment of collagen XVIII that accumulates in the brain of patients with Alzheimer's disease (AD), in the cerebrospinal fluids (CSF) of patients with neurodegenerative diseases. The concentrations of total protein, endostatin, amyloid-β1-42 peptide, tau, and hyperphosphorylated tau proteins were measured by enzyme-linked immunosorbent assay in CSF of patients with AD (n = 57), behavioral frontotemporal dementia (bvFTD, n = 22), non AD and non FTD dementia (nAD/nFTD, n = 84), and 45 subjects without neurodegenerative diseases. The statistical significance of the results was assessed by Mann-Whitney and Kruskal and Wallis tests, and by ROC analysis. The concentration of endostatin in CSF was higher than the levels of the three markers of AD both in control subjects and in patients with neurodegenerative diseases. The endostatin/amyloid-β1-42 ratio was significantly increased in patients with AD (257%, p < 0.0001) and nAD/nFTD (140%, p < 0.0001) compared to controls. The endostatin/tau protein ratio was significantly decreased in patients with AD (-49%, p < 0.0001) but was increased in bvFTD patients (89%, p < 0.0001) compared to controls. In the same way, the endostatin/hyperphosphorylated tau protein ratio was decreased in patients with AD (-21%, p = 0.0002) but increased in patients with bvFTD (81%, p = 0.0026), compared to controls. The measurement of endostatin in CSF and the calculation of its ratio relative to well-established AD markers improve the diagnosis of bvFTD patients and the discrimination of patients with AD from those with bvFTD and nAD/nFTD.
authors.affiliation: Bases Moléculaires et Structurales des Systèmes Infectieux, UMR 5086 CNRS, Université Lyon 1, Institut de Biologie et Chimie des Protéines, Lyon, France.
authors.forename: Romain
authors.initials: R
authors.lastname: Salza
authors.affiliation: CHU de Reims, Laboratoire Central de Biochimie, Reims, France Université de Reims Champagne-Ardenne, CNRS UMR 7369, Matrice Extracellulaire et Dynamique Cellulaire, Reims, France.
authors.forename: Jean-Baptiste
authors.initials: JB
authors.lastname: Oudart
authors.affiliation: CHU de Reims, Laboratoire Central de Biochimie, Reims, France Université de Reims Champagne-Ardenne, CNRS UMR 7369, Matrice Extracellulaire et Dynamique Cellulaire, Reims, France.
authors.forename: Laurent
authors.initials: L
authors.lastname: Ramont
authors.affiliation: CHU de Reims, Laboratoire Central de Biochimie, Reims, France Université de Reims Champagne-Ardenne, CNRS UMR 7369, Matrice Extracellulaire et Dynamique Cellulaire, Reims, France.
authors.forename: François-Xavier
authors.initials: FX
authors.lastname: Maquart
authors.affiliation: CHU de Reims, Service de Neurologie, Centre Mémoire de Ressources et de Recherche Champagne-Ardenne (CMRR), Reims, France.
authors.forename: Serge
authors.initials: S
authors.lastname: Bakchine
authors.affiliation: CHU de Reims, Laboratoire Central de Biochimie, Reims, France.
authors.forename: Henri
authors.initials: H
authors.lastname: Thoannès
authors.affiliation: Bases Moléculaires et Structurales des Systèmes Infectieux, UMR 5086 CNRS, Université Lyon 1, Institut de Biologie et Chimie des Protéines, Lyon, France.
authors.forename: Sylvie
authors.initials: S
authors.lastname: Ricard-Blum
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D000544:Alzheimer Disease / Q000134:cerebrospinal fluid*; D016229:Amyloid beta-Peptides / Q000134:cerebrospinal fluid; D043169:Endostatins / Q000134:cerebrospinal fluid*; D005260:Female; D057180:Frontotemporal Dementia / Q000134:cerebrospinal fluid; D006801:Humans; D008297:Male; D008875:Middle Aged; D010446:Peptide Fragments / Q000134:cerebrospinal fluid; D010766:Phosphorylation; D011569:Psychiatric Status Rating Scales; D012372:ROC Curve; D016875:tau Proteins / Q000134:cerebrospinal fluid
chemical_list: D016229:Amyloid beta-Peptides; D043169:Endostatins; D010446:Peptide Fragments; C075222:amyloid beta-protein (1-42); D016875:tau Proteins
keywords: Alzheimer's disease; amyloid-$\beta_{1-42}$ peptide; biomarkers; cerebrospinal fluid; endostatin; hyperphosphorylated tau; neurodegenerative diseases; tau