title: Physiological characteristics of crab Portunus sanguinolentus egg mass extract from southeast coast of India.
abstract: BACKGROUND
  The present investigation was undertaken to evaluate the physiological characteristics of crab egg mass (Portunus sanguinolentus) crude extract.
  
  
  METHODS
  Assays were followed by standard methods.
  
  
  RESULTS
  A maximum of 256 hemolytic units (HU) and a minimum of 128 HU were found in chicken and goat erythrocytes. In an antimicrobial assay, Salmonella paratyphi and Aspergillus niger showed most susceptibility (8.0 mm and 13.0 mm) against n-butanol extracts. In the tail flick method, 5.33±0.19* AR the maximum analgesic response was recorded 30 min after the administration of crude extract (150 mg kg-1). In the hot plate method, 5.13±0.13* AR maximum paw licking was recorded 15 min after the administration of crude extract (150 mg kg-1). In an anti-inflammatory assay, 0.31±0.23* anti-inflammatory response and inhibition of paw edema 39.22% were observed 1 h after the administration of crude extract (250 mg kg-1). Primary structural groups gained through Fourier transformed infrared (FTIR) analysis peaks were observed and assigned as amide groups I (1517.81-1633.12 cm-1), II (1446.58-1517.81 cm-1) and III (1342.47-1406.85 cm-1).
  
  
  CONCLUSIONS
  The results indicate that crab egg masses have remarkable antimicrobial, hemolytic and cytotoxic activities.
authors.forename: Subramanian
authors.initials: S
authors.lastname: Bragadeeswaran
authors.forename: Nadarajah Sri
authors.initials: NS
authors.lastname: Kumaran
authors.forename: Lakshmanan
authors.initials: L
authors.lastname: Pandimuthu
authors.forename: Rajagopal
authors.initials: R
authors.lastname: Prabahar
mesh_terms: D000700:Analgesics / Q000494:pharmacology; D000818:Animals; D000890:Anti-Infective Agents / Q000494:pharmacology; D000893:Anti-Inflammatory Agents / Q000494:pharmacology; D001234:Aspergillus niger / Q000187:drug effects; D003386:Brachyura / Q000737:chemistry*; D002645:Chickens; D045424:Complex Mixtures / Q000494:pharmacology*; D004487:Edema / Q000188:drug therapy; D004531:Eggs; D004912:Erythrocytes / Q000187:drug effects; D006041:Goats; D053878:Hemolytic Agents / Q000494:pharmacology; D007194:India; D051379:Mice; D012482:Salmonella paratyphi A / Q000187:drug effects
chemical_list: D000700:Analgesics; D000890:Anti-Infective Agents; D000893:Anti-Inflammatory Agents; D045424:Complex Mixtures; D053878:Hemolytic Agents