title: [Purification of recombinant Bacillus cereus ResD-ResE proteins expressed in Escherichia coli strains].
abstract: Recombinant E. coli strains expressing the Bacillus cereus ATCC 14579T resD and resEgenes fused with the ubiquitin gene were constructed, and purification of the ResD and ResE proteins was performed. The approach used in the study allowed us to increase the protein yield of the electrophoretic homogeneous ResD andResE proteins without denaturation steps up to 150 mg per gram of wet cell weight.
authors.forename: E V
authors.initials: EV
authors.lastname: Shapyrina
authors.forename: A M
authors.initials: AM
authors.lastname: Shadrin
authors.forename: A S
authors.initials: AS
authors.lastname: Solonin
mesh_terms: D001409:Bacillus cereus / Q000235:genetics* / Q000378:metabolism; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D014157:Transcription Factors / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification
chemical_list: D001426:Bacterial Proteins; D011993:Recombinant Fusion Proteins; D014157:Transcription Factors