title: CHoMP: a chemoenzymatic histology method using clickable probes.
abstract: The characterization of aberrant glycosylation patterns in biopsied patient samples represents a remarkable challenge for scientists and medical doctors due to the lack of specific methods for detection. Here, we report the development of a histological method, dubbed CHoMP-chemoenzymatic histology of membrane polysaccharides-for analyzing glycosylation patterns in mammalian tissues. This method exploits a recombinant glycosyltransferase to transfer a monosaccharide analogue equipped with a chemical handle to a specific cell-surface glycan target, which can then be derivatized with imaging probes by using bioorthogonal click chemistry for visualization. We applied CHoMP to survey changes in expression of N-acetyllactosamine (LacNAc) in human samples from patients afflicted with lung adenocarcinoma and observed a sharp decrease in expression levels between normal and early grade tumors, thus suggesting a potential application of this technique in early cancer diagnosis.
authors.affiliation: Department of Biochemistry, Albert Einstein College of Medicine, Price Center for Genetics and Translational Medicine, 1301 Morris Park Avenue, Room 513, Bronx, NY 10461 (USA).
authors.forename: Sara H
authors.initials: SH
authors.lastname: Rouhanifard
authors.forename: Aimé
authors.initials: A
authors.lastname: López-Aguilar
authors.forename: Peng
authors.initials: P
authors.lastname: Wu
mesh_terms: D000230:Adenocarcinoma / Q000175:diagnosis* / Q000473:pathology; D000606:Amino Sugars / Q000032:analysis*; D000818:Animals; D057930:Click Chemistry / Q000379:methods; D006031:Glycosylation; D006652:Histological Techniques / Q000379:methods; D006801:Humans; D008168:Lung / Q000473:pathology*; D008175:Lung Neoplasms / Q000175:diagnosis* / Q000473:pathology; D008297:Male; D008810:Mice, Inbred C57BL
chemical_list: D000606:Amino Sugars; C000458:N-acetyllactosamine
keywords: CuAAC; click chemistry; glycobiology; histology; lung cancer