title: Drug transporter expression during in vitro differentiation of first-trimester and term human villous trophoblasts.
abstract: Drug transporters interfere with drug disposition during pregnancy by actively transporting drugs from mother to fetus, and vice versa. Data on their placental expression are scarce, especially during the first trimester of pregnancy. The aim of our study was to assess mRNA expression of more than 80 drug transporters by using an RT-qPCR array in primary cytotrophoblastic cells isolated from first-trimester and term human placentas and cultured for 72 h to form syncytiotrophoblasts. This original expression panel of human placental drug transporters should help to understand transplacental drug transfer and to ensure more rational drug use during pregnancy.
authors.affiliation: INSERM, UMR-S1139, Paris, France; Université Paris Descartes, Sorbonne Paris Cité, Paris, France.
authors.forename: P
authors.initials: P
authors.lastname: Berveiller
authors.affiliation: INSERM, UMR-S1139, Paris, France; Université Paris Descartes, Sorbonne Paris Cité, Paris, France.
authors.forename: S A
authors.initials: SA
authors.lastname: Degrelle
authors.affiliation: INSERM, UMR-S1139, Paris, France; Université Paris Descartes, Sorbonne Paris Cité, Paris, France.
authors.forename: N
authors.initials: N
authors.lastname: Segond
authors.affiliation: Institut Mutualiste Montsouris, Paris, France.
authors.forename: H
authors.initials: H
authors.lastname: Cohen
authors.affiliation: INSERM, UMR-S1139, Paris, France; Université Paris Descartes, Sorbonne Paris Cité, Paris, France; PremUp Foundation, Paris, France.
authors.forename: D
authors.initials: D
authors.lastname: Evain-Brion
authors.affiliation: INSERM, UMR-S1139, Paris, France; Université Paris Descartes, Sorbonne Paris Cité, Paris, France; PremUp Foundation, Paris, France. Electronic address: sophie.gil@parisdescartes.fr.
authors.forename: S
authors.initials: S
authors.lastname: Gil
mesh_terms: D001692:Biological Transport; D002454:Cell Differentiation / Q000502:physiology*; D005260:Female; D006801:Humans; D026901:Membrane Transport Proteins / Q000378:metabolism*; D010920:Placenta / Q000378:metabolism; D011247:Pregnancy; D011261:Pregnancy Trimester, First / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D014327:Trophoblasts / Q000166:cytology / Q000378:metabolism*
chemical_list: D026901:Membrane Transport Proteins; D012333:RNA, Messenger
keywords: ABC transporters; Cytotrophoblast; Placenta; SLC transporters; Syncytiotrophoblast