title: Lipopolysaccharide-binding protein and leptin are associated with stress-induced interleukin-6 cytokine expression ex vivo in obesity.
abstract: Obesity is associated with enhanced inflammation and mental stress, but limited information has addressed the potential additive effect of psychological stress on obesity-associated inflammation. This study examined whether obese subjects would elicit a greater host immune response (IL-6 mRNA and cytokine) to lipopolysaccharide (LPS) in response to mental stress. Blood samples for LPS-stimulated IL-6 mRNA and cytokine were collected prior to and following mental stress. Results showed that obese subjects elicited a greater LPS-induced IL-6 along with its mRNA expression following mental stress compared to normal-weight subjects. Stress-induced IL-6 cytokine response to LPS was correlated with the baseline levels of plasma LPS binding protein (LBP) and leptin. These findings are consistent with the idea that endogenous inflammatory agents (e.g., LBP and leptin), often elevated with obesity, enhance inflammatory responses to psychological stress.
authors.affiliation: Department of Exercise Science and Health Promotion, Florida Atlantic University, Boca Raton, Florida, USA.
authors.forename: Chun-Jung
authors.initials: CJ
authors.lastname: Huang
authors.forename: Jennifer K
authors.initials: JK
authors.lastname: Stewart
authors.forename: Yoshimi
authors.initials: Y
authors.lastname: Shibata
authors.forename: Aaron L
authors.initials: AL
authors.lastname: Slusher
authors.forename: Edmund O
authors.initials: EO
authors.lastname: Acevedo
mesh_terms: D000209:Acute-Phase Proteins; D000328:Adult; D002352:Carrier Proteins / Q000097:blood*; D006801:Humans; D015850:Interleukin-6 / Q000097:blood*; D020738:Leptin / Q000097:blood*; D008297:Male; D008562:Membrane Glycoproteins / Q000097:blood*; D009765:Obesity / Q000097:blood*; D013315:Stress, Psychological / Q000097:blood*; D000099231:Lipopolysaccharide-Binding Protein
chemical_list: D000209:Acute-Phase Proteins; D002352:Carrier Proteins; D015850:Interleukin-6; D020738:Leptin; D008562:Membrane Glycoproteins; D000099231:Lipopolysaccharide-Binding Protein; C508600:IL6 protein, human
keywords: Interleukin-6; Leptin; Lipopolysaccharide-binding protein; Obesity; Psychological stress