title: Simultaneous analysis of PhIP, 4'-OH-PhIP, and their precursors using UHPLC-MS/MS.
abstract: A novel method allowing simultaneous analysis of PhIP, 4'-OH-PhIP, and their precursors (phenylalanine, tyrosine, creatine, creatinine, glucose) has been developed as a robust kinetic study tool by using ultra high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS). A direct hydrochloric acid (HCl) extraction was applied to achieve the simultaneous extraction of all seven analytes, with the mean recoveries ranging from 60% to 120% at two concentration levels. Then, an Atlantis dC18 column selected from four different chromatographic columns was ultimately used to separate these compounds within 15 min. The limits of detection range of allseven analytes were calculated as 0.14-325.00 μg L(-1). The intra- and interday precision of the proposed method were less than 15.4 and 19.9%, respectively. The proposed method was successfully applied to depict the kinetic profiles of PhIP, 4'-OH-PhIP, and their precursors in pork model, reducing the analysis time and cost in the kinetic study.
authors.affiliation: Key Laboratory of Food Science and Technology, Jiangnan University , Wuxi 214122, People's Republic of China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Yan
authors.forename: Mao-Mao
authors.initials: MM
authors.lastname: Zeng
authors.forename: Zong-Ping
authors.initials: ZP
authors.lastname: Zheng
authors.forename: Zhi-Yong
authors.initials: ZY
authors.lastname: He
authors.forename: Guan-Jun
authors.initials: GJ
authors.lastname: Tao
authors.forename: Shuang
authors.initials: S
authors.lastname: Zhang
authors.forename: Ya-Hui
authors.initials: YH
authors.lastname: Gao
authors.forename: Jie
authors.initials: J
authors.lastname: Chen
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003296:Cooking; D003401:Creatine / Q000032:analysis; D003404:Creatinine / Q000032:analysis; D005947:Glucose / Q000032:analysis; D006358:Hot Temperature; D007093:Imidazoles / Q000032:analysis*; D008460:Meat / Q000032:analysis*; D010649:Phenylalanine / Q000032:analysis; D013552:Swine; D053719:Tandem Mass Spectrometry / Q000379:methods*; D014443:Tyrosine / Q000032:analysis
chemical_list: D007093:Imidazoles; D014443:Tyrosine; D010649:Phenylalanine; C049584:2-amino-1-methyl-6-phenylimidazo(4,5-b)pyridine; D003404:Creatinine; D005947:Glucose; D003401:Creatine
keywords: UHPLC−MS/MS; heterocyclic aromatic amines; meat model; precursors; simultaneous analysis