title: S. sanguinis adhesion on rough titanium surfaces: effect of culture media.
abstract: Bacterial colonization plays a key role in dental implant failure, because they attach directly on implant surface upon implantation. Between different types of bacteria associated with the oral environment, Streptococcus sanguinis is essential in this process since it is an early colonizer. In this work the relationship between titanium surfaces modified by shot blasting treatment and S. sanguinis adhesion; have been studied in approached human mouth environment. Bacteria pre-inoculated with routinary solution were put in contact with titanium samples, shot-blasted with alumina and silicon carbide, and adhesion results were compared with those obtained when bacteria were pre-inoculated with modified artificial saliva medium and on saliva pre-coated titanium samples. Our results showed that bacterial adhesion on titanium samples was influenced by culture conditions. When S. sanguinis was inoculated in routinary culture media, colonies forming unities per square millimeter presented an increment correlated with roughness and surface energy, but separated by the type of particle used during shot-blasting treatment; whereas in modified artificial saliva only a relationship between bacteria adhered and the increment in both roughness and surface energy were observed, regardless of the particle type. Finally, on human saliva pre-coated samples no significant differences were observed among roughness, surface energy or particle.
authors.affiliation: Microbiology and Parasitology Department, Medicine Faculty, National Autonomous University of Mexico (UNAM), México DF, Mexico. Electronic address: a.g.rodriguez.hernandez@gmail.com.
authors.forename: Ana G
authors.initials: AG
authors.lastname: Rodríguez-Hernández
authors.affiliation: Institute of Physics, National Autonomous University of México (UNAM), Circuito de la Investigación Científica s/n, Cd Universitaria, 04510 México DF, Mexico.
authors.forename: José A
authors.initials: JA
authors.lastname: Muñoz-Tabares
authors.affiliation: Biomaterials, Biomechanics and Tissue Engineering Group (BIBITE), Department of Materials Science and Metallurgy, Universitat Politècnica de Catalunya, Diagonal Av. 647, 08028 Barcelona, Spain.
authors.forename: Maria
authors.initials: M
authors.lastname: Godoy-Gallardo
authors.affiliation: Microbiology Department, Faculty of Biology, University of Barcelona, Av Diagonal 645, 08028 Barcelona, Spain; Institut de Bioenginyeria de Catalunya (IBEC), Parc Cientific of Barcelona, C/Josep Samitier 1-5, 08028 Barcelona, Spain.
authors.forename: Antonio
authors.initials: A
authors.lastname: Juárez
authors.affiliation: Biomaterials, Biomechanics and Tissue Engineering Group (BIBITE), Department of Materials Science and Metallurgy, Universitat Politècnica de Catalunya, Diagonal Av. 647, 08028 Barcelona, Spain.
authors.forename: Francisco-Javier
authors.initials: FJ
authors.lastname: Gil
mesh_terms: D001422:Bacterial Adhesion; D003470:Culture Media / Q000737:chemistry*; D015921:Dental Implants; D006801:Humans; D008855:Microscopy, Electron, Scanning; D012463:Saliva / Q000737:chemistry; D012464:Saliva, Artificial / Q000737:chemistry; D013298:Streptococcus sanguis / Q000502:physiology*; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry*
chemical_list: D003470:Culture Media; D015921:Dental Implants; D012464:Saliva, Artificial; D014025:Titanium