title: Quantitative chemical exchange saturation transfer with hyperpolarized nuclei (qHyper-CEST): sensing xenon-host exchange dynamics and binding affinities by NMR.
abstract: The reversible binding of xenon to host molecules has found numerous applications in nuclear magnetic resonance studies. Quantitative characterization of the Xe exchange dynamics is important to understand and optimize the physico-chemical behavior of such Xe hosts, but is often challenging to achieve at low host concentrations. We have investigated a sensitive quantification technique based on chemical exchange saturation transfer with hyperpolarized nuclei, qHyper-CEST. Using simulated signals we demonstrated that qHyper-CEST yielded accurate and precise results and was robust in the presence of large amounts of noise (10%). This is of particular importance for samples with completely unknown exchange rates. Using these findings we experimentally determined the following exchange parameters for the Xe host cryptophane-A monoacid in dimethyl sulfoxide in one type of experiment: the ratio of bound and free Xe, the Xe exchange rate, the resonance frequencies of free and bound Xe, the Xe host occupancy, and the Xe binding constant. Taken together, qHyper-CEST facilitates sensitive quantification of the Xe exchange dynamics and binding to hydrophobic cavities and has the potential to analyze many different host systems or binding sites. This makes qHyper-CEST an indispensable tool for the efficient design of highly specific biosensors.
authors.affiliation: ERC Project BiosensorImaging, Leibniz-Institut für Molekulare Pharmakologie (FMP), 13125 Berlin, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Kunth
authors.affiliation: ERC Project BiosensorImaging, Leibniz-Institut für Molekulare Pharmakologie (FMP), 13125 Berlin, Germany.
authors.forename: C
authors.initials: C
authors.lastname: Witte
authors.affiliation: ERC Project BiosensorImaging, Leibniz-Institut für Molekulare Pharmakologie (FMP), 13125 Berlin, Germany.
authors.forename: L
authors.initials: L
authors.lastname: Schröder
mesh_terms: D001665:Binding Sites; D004121:Dimethyl Sulfoxide / Q000737:chemistry; D057927:Hydrophobic and Hydrophilic Interactions; D057230:Limit of Detection; D008279:Magnetic Resonance Imaging; D008962:Models, Theoretical; D011083:Polycyclic Compounds / Q000737:chemistry; D014978:Xenon / Q000737:chemistry*
chemical_list: D011083:Polycyclic Compounds; C545484:cryptophane A; D014978:Xenon; D004121:Dimethyl Sulfoxide