title: Phosphatidylethanolamine plasmalogen enhances the inhibiting effect of phosphatidylethanolamine on γ-secretase activity.
abstract: Plasmalogens (Pls) are widely distributed in the biological membrane of animals and certain anaerobic bacteria, but their functions in the cell membrane are still poorly understood. Decrease of phosphatidylethanolamine plasmalogen (PEPls) in the brain tissue of patients with Alzheimer's disease prompted us to investigate the effect of the membrane phosphorus lipid composition on the activity of γ-secretase that produces amyloid-beta protein (Aβ). To clarify the effect of phospholipids, including PEPls, on Aβ production, γ-secretase activity was measured in an in vitro assay using yeast microsomes and reconstituted liposomes. The presence of ethanolamine phospholipids in the proteoliposome weakened γ-secretase activity. In addition, increased PEPls content in total ethanolamine phospholipids further decreased the enzyme activity, indicating that γ-secretase activity is affected by the membrane phospholipid PEPls/PE ratio. Furthermore, PEPls from anaerobic bacterial cell membrane induced the same effect on γ-secretase activity.
authors.affiliation: Department of Microbial Biotechnology and Department of Molecular and Cell Biology, Graduate School of Agricultural Science, Tohoku University, 1-1 Tsutsumidori-Amamiyamachi, Sendai 981-8555, Japan; and Department of Human Health and Nutrition, Graduate School of Comprehensive Human Sciences, Shokei Gakuin University, Yurigaoka 4-10-1, Natori 981-1295, Japan.
authors.forename: Tomoko
authors.initials: T
authors.lastname: Onodera
authors.affiliation: Department of Microbial Biotechnology and Department of Molecular and Cell Biology, Graduate School of Agricultural Science, Tohoku University, 1-1 Tsutsumidori-Amamiyamachi, Sendai 981-8555, Japan; and Department of Human Health and Nutrition, Graduate School of Comprehensive Human Sciences, Shokei Gakuin University, Yurigaoka 4-10-1, Natori 981-1295, Japan.
authors.forename: Eugene
authors.initials: E
authors.lastname: Futai
authors.affiliation: Department of Microbial Biotechnology and Department of Molecular and Cell Biology, Graduate School of Agricultural Science, Tohoku University, 1-1 Tsutsumidori-Amamiyamachi, Sendai 981-8555, Japan; and Department of Human Health and Nutrition, Graduate School of Comprehensive Human Sciences, Shokei Gakuin University, Yurigaoka 4-10-1, Natori 981-1295, Japan.
authors.forename: Eiichiro
authors.initials: E
authors.lastname: Kan
authors.affiliation: Department of Microbial Biotechnology and Department of Molecular and Cell Biology, Graduate School of Agricultural Science, Tohoku University, 1-1 Tsutsumidori-Amamiyamachi, Sendai 981-8555, Japan; and Department of Human Health and Nutrition, Graduate School of Comprehensive Human Sciences, Shokei Gakuin University, Yurigaoka 4-10-1, Natori 981-1295, Japan.
authors.forename: Naoki
authors.initials: N
authors.lastname: Abe
authors.affiliation: Department of Microbial Biotechnology and Department of Molecular and Cell Biology, Graduate School of Agricultural Science, Tohoku University, 1-1 Tsutsumidori-Amamiyamachi, Sendai 981-8555, Japan; and Department of Human Health and Nutrition, Graduate School of Comprehensive Human Sciences, Shokei Gakuin University, Yurigaoka 4-10-1, Natori 981-1295, Japan.
authors.forename: Takafumi
authors.initials: T
authors.lastname: Uchida
authors.affiliation: Department of Microbial Biotechnology and Department of Molecular and Cell Biology, Graduate School of Agricultural Science, Tohoku University, 1-1 Tsutsumidori-Amamiyamachi, Sendai 981-8555, Japan; and Department of Human Health and Nutrition, Graduate School of Comprehensive Human Sciences, Shokei Gakuin University, Yurigaoka 4-10-1, Natori 981-1295, Japan.
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Kamio
authors.affiliation: Department of Microbial Biotechnology and Department of Molecular and Cell Biology, Graduate School of Agricultural Science, Tohoku University, 1-1 Tsutsumidori-Amamiyamachi, Sendai 981-8555, Japan; and Department of Human Health and Nutrition, Graduate School of Comprehensive Human Sciences, Shokei Gakuin University, Yurigaoka 4-10-1, Natori 981-1295, Japan jkaneko@biochem.tohoku.ac.jp.
authors.forename: Jun
authors.initials: J
authors.lastname: Kaneko
mesh_terms: D053829:Amyloid Precursor Protein Secretases / Q000378:metabolism*; D000818:Animals; D006801:Humans; D010714:Phosphatidylethanolamines / Q000378:metabolism*; D010955:Plasmalogens / Q000502:physiology*; D011510:Proteolipids
chemical_list: D010714:Phosphatidylethanolamines; D010955:Plasmalogens; D011510:Proteolipids; C020791:phosphatidal ethanolamines; C020485:proteoliposomes; C483858:phosphatidylethanolamine; D053829:Amyloid Precursor Protein Secretases
keywords: Alzheimer's disease; amyloid-beta; phosphatidylethanolamine plasmalogen; yeast microsomes; γ-secretase activity