title: Efficacy of combined therapy of periosteum and bone allograft in a critical-sized defect model in New Zealand white rabbits.
abstract: BACKGROUND
  Large segmental bone defects caused by trauma, infection, or bone tumor resection are difficult to cure and have been a problem in the field of bone repair for decades. The objective of this study was to discuss the efficacy of combined therapy of free periosteum and bone allograft in treating bone defects and to provide a theoretical basis for clinical application of this therapy.
  
  
  METHODS
  A unilateral tibia cortical defect model in New Zealand white rabbits was established according to Girolamo method. Total 48 rabbits were randomized into 3 groups: a simple bone defect group (n=16), an autogenous bone graft group (n=16), and a periosteum and bone allograft combined therapy group (n=16). The efficacy was evaluated by imaging inspections and scoring, HE staining, and RT-PCR in postoperative weeks 2, 4, 8, and 12.
  
  
  RESULTS
  The results of imaging and histopathological inspections in the study indicated that in postoperative weeks 4, 8, and 12 the experimental and control groups had statistically significant differences in Lane-Sandhu radiographic scoring and relative bone density when compared with the simple bone defect group (P<0.05). The RT-PCR results suggested that the expression of SPP-1, BMP-2, and VEGF in the experimental group was higher than in the control group (P<0.05) and the expression of Col Ia1 in the control group was higher than in the experimental group (P<0.05).
  
  
  CONCLUSIONS
  Efficacies of the combined therapy (periosteum combined with bone allografting) and the criterion standard therapy (autogenous bone grafting) are equivalent in treating bone defects in New Zealand white rabbits.
authors.affiliation: Department of Traumatic and Microsurgical, The Third Affiliated Hospital of Southern Medical University and Second People's Hospital of Guangdong Province, Guangzhou, China (mainland).
authors.forename: Dawei
authors.initials: D
authors.lastname: Zhang
authors.affiliation: Department of Traumatic and Microsurgical, The Third Affiliated Hospital of Southern Medical University and Second People's Hospital of Guangdong Province, Guangzhou, China (mainland).
authors.forename: Dong
authors.initials: D
authors.lastname: Huang
authors.affiliation: Department of Traumatic and Microsurgical, The Third Affiliated Hospital of Southern Medical University and Second People's Hospital of Guangdong Province, Guangzhou, China (mainland).
authors.forename: Yongjun
authors.initials: Y
authors.lastname: Huang
authors.affiliation: Department of Traumatic and Microsurgical, The Third Affiliated Hospital of Southern Medical University and Second People's Hospital of Guangdong Province, Guangzhou, China (mainland).
authors.forename: Yuanhang
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Traumatic and Microsurgical, The Third Affiliated Hospital of Southern Medical University and Second People's Hospital of Guangdong Province, Guangzhou, China (mainland).
authors.forename: Bochuan
authors.initials: B
authors.lastname: Lin
authors.affiliation: Department of Traumatic and Microsurgical, The Third Affiliated Hospital of Southern Medical University and Second People's Hospital of Guangdong Province, Guangzhou, China (mainland).
authors.forename: Chaoqun
authors.initials: C
authors.lastname: Yu
authors.affiliation: Department of Traumatic and Microsurgical, The Third Affiliated Hospital of Southern Medical University and Second People's Hospital of Guangdong Province, Guangzhou, China (mainland).
authors.forename: Yong
authors.initials: Y
authors.lastname: Mou
authors.affiliation: Department of Traumatic and Microsurgical, The Third Affiliated Hospital of Southern Medical University and Second People's Hospital of Guangdong Province, Guangzhou, China (mainland).
authors.forename: Weichi
authors.initials: W
authors.lastname: Wu
authors.affiliation: Department of Traumatic and Microsurgical, The Third Affiliated Hospital of Southern Medical University and Second People's Hospital of Guangdong Province, Guangzhou, China (mainland).
authors.forename: Huiru
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Department of Traumatic and Microsurgical, The Third Affiliated Hospital of Southern Medical University and Second People's Hospital of Guangdong Province, Guangzhou, China (mainland).
authors.forename: Hao
authors.initials: H
authors.lastname: Lin
mesh_terms: D064591:Allografts; D000818:Animals; D015519:Bone Density; D001847:Bone Diseases / Q000000981:diagnostic imaging / Q000503:physiopathology / Q000628:therapy*; D016025:Bone Transplantation; D004195:Disease Models, Animal; D005453:Fluorescence; D021621:Imaging, Three-Dimensional; D010521:Periosteum / Q000637:transplantation*; D016133:Polymerase Chain Reaction; D011182:Postoperative Care; D011817:Rabbits; D011859:Radiography; D013977:Tibia / Q000000981:diagnostic imaging / Q000473:pathology* / Q000503:physiopathology; D016896:Treatment Outcome