title: miRNA-205 is a candidate tumor suppressor that targets ZEB2 in renal cell carcinoma.
abstract: BACKGROUND
  This study aims to characterize the function of downregulated MicroRNA miR-205 in renal cell carcinoma (RCC), and show how the downstream zinc finger E-box-binding homeobox 2 (ZEB2) is negatively regulated by miR-205.
  
  
  METHODS
  The expression of miR-205 was detected in RCC and adjacent non-tumor tissues using real-time polymerase chain reaction (PCR). The expression of miR-205 and ZEB2 was detected in RCC cell lines using real-time PCR. The luciferase reporter assay was used to assess ZEB2 as a target of miR-205. Protein levels of ZEB2, E-cadherin, and vimentin were measured by western blot after overexpression of miR-205 in ACHN cells. In vivo functions of miR-205 in ACHN cells were measured by MTT assays, migration and invasion assays, and flow cytometry.
  
  
  RESULTS
  MiR-205 was significantly downregulated in RCC samples and cell lines compared with matched non-tumor tissues and HK-2 cells, respectively. No significant difference was found in miR-205 expression between well differentiated and poorly to moderately differentiated groups or between phase I and phase II-III. ZEB2 was upregulated in RCC cell lines compared with expression in HK-2 cells. Upregulation of miR-205 expression caused the downregulation of ZEB2 and vimentin, and the upregulation of E-cadherin in ACHN cells. miR-205 also inhibited proliferation, migration, and invasion, and induced apoptosis of ACHN cells.
  
  
  CONCLUSIONS
  miR-205 is a candidate tumor suppressor that targets ZEB2 in RCC.
authors.affiliation: Department of Urology, Xiangya Hospital, Central South University, Changsha, China.
authors.forename: Zhi
authors.initials: Z
authors.lastname: Chen
authors.forename: Zheng-Yan
authors.initials: ZY
authors.lastname: Tang
authors.forename: Yao
authors.initials: Y
authors.lastname: He
authors.forename: Long-Fei
authors.initials: LF
authors.lastname: Liu
authors.forename: Dong-Jie
authors.initials: DJ
authors.lastname: Li
authors.forename: Xiang
authors.initials: X
authors.lastname: Chen
mesh_terms: D000328:Adult; D000368:Aged; D017209:Apoptosis; D015153:Blotting, Western; D015820:Cadherins / Q000378:metabolism; D002292:Carcinoma, Renal Cell / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D045744:Cell Line, Tumor; D002465:Cell Movement; D015536:Down-Regulation / Q000502:physiology; D005260:Female; D005434:Flow Cytometry; D016147:Genes, Tumor Suppressor / Q000502:physiology*; D018398:Homeodomain Proteins / Q000378:metabolism*; D006801:Humans; D007680:Kidney Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D060888:Real-Time Polymerase Chain Reaction; D012097:Repressor Proteins / Q000378:metabolism*; D014162:Transfection; D014407:Tumor Cells, Cultured; D014746:Vimentin / Q000378:metabolism; D000074165:Zinc Finger E-box Binding Homeobox 2
chemical_list: D015820:Cadherins; D018398:Homeodomain Proteins; D035683:MicroRNAs; D012097:Repressor Proteins; D014746:Vimentin; D000074165:Zinc Finger E-box Binding Homeobox 2; C534425:MIR205, human; C492363:ZEB2 protein, human