title: Sugarcane (Saccharum spp. hybrids).
abstract: Genetic transformation of sugarcane has a tremendous potential to complement traditional breeding in crop improvement and will likely transform sugarcane into a bio-factory for value-added products. We describe here Agrobacterium tumefaciens-mediated transformation of sugarcane. Embryogenic callus induced from immature leaf whorls was used as target for transformation with the hypervirulent Agrobacterium strain AGL1 carrying a constitutive nptII expression cassette in vector pPZP200. Selection with 30 mg/L geneticin during the callus phase and 30 mg/L paromomycin during regeneration of shoots and roots effectively suppressed the development of non-transgenic plants. This protocol was successful with a commercially important sugarcane cultivar, CP-88-1762, at a transformation efficiency of two independent transgenic plants per g of callus.
authors.affiliation: Agronomy Department, Plant Molecular and Cellular Biology Program, Genetics Institute, University of Florida, 3085 McCarty Hall, 110500, Gainesville, FL, 32611-0500, USA.
authors.forename: Hao
authors.initials: H
authors.lastname: Wu
authors.forename: Fredy
authors.initials: F
authors.lastname: Altpeter
mesh_terms: D016960:Agrobacterium tumefaciens / Q000235:genetics / Q000254:growth & development; D018920:Coculture Techniques; D004780:Environment, Controlled; D005818:Genetic Engineering / Q000379:methods*; D012038:Regeneration; D031786:Saccharum / Q000235:genetics* / Q000254:growth & development* / Q000502:physiology; D014170:Transformation, Genetic