title: Physical and chemical characterization of adsorbed protein onto gold electrode functionalized with Tunisian coral and nacre.
abstract: Bone substitutes are more and more used in bone surgery because of their biologic safety, clinic efficiency and facility to synthesize. Bone substitutes with active osteogenic properties, associating biomaterials with organic macromolecule components of the extracellular matrix (protein, GAG) are recommended. Nevertheless, we should have a simple technique to control interactions between proteins and the material. Natural coral and nacre have been found to be impressive bone graft substitutes. In this work, we characterize nacre and coral powder using energy dispersive X-ray analysis (EDX). We used electrochemical impedance spectroscopy (EIS) and attenuated total reflectance Fourier transform infrared (ATR-FTIR) spectroscopy to evaluate bovine serum albumin (BSA) as model protein, adsorbed to these biomaterial surfaces. In order to understand the nacre/coral-protein interfacial compatibility, it is necessary to investigate the wettability.
authors.affiliation: Biomaterials and Biomechanics Laboratory, National Institute M.T. Kassab of Orthopedic, 2010 La Manouba, Tunis, Tunisia. samir.hamza@insat.rnu.tn
authors.forename: Samir
authors.initials: S
authors.lastname: Hamza
authors.affiliation: National Institute of Applied Sciences and Technology, Centre Urbain Nord, Box 676, 1080 Tunis cedex, Tunisia. Electronic address: bouchemimeryem@yahoo.fr.
authors.forename: Meryem
authors.initials: M
authors.lastname: Bouchemi
authors.affiliation: Biomaterials and Biomechanics Laboratory, National Institute M.T. Kassab of Orthopedic, 2010 La Manouba, Tunis, Tunisia. Electronic address: labiomecanique@yahoo.fr.
authors.forename: Noureddine
authors.initials: N
authors.lastname: Slimane
authors.affiliation: Laboratory of Biomechanics, Polymer and Structures Mechanics, National School of Engineers of Metz, France, 1 route d'Ars Laquenexy, CS 65820 57078 Metz cedex 03, France. Electronic address: azari@univ-metz.fr.
authors.forename: Zitouni
authors.initials: Z
authors.lastname: Azari
mesh_terms: D000327:Adsorption; D000818:Animals; D037421:Anthozoa / Q000737:chemistry*; D002417:Cattle; D058266:Dielectric Spectroscopy; D055664:Electrochemical Techniques; D004566:Electrodes; D006046:Gold / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D060734:Nacre / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D013052:Spectrometry, X-Ray Emission; D017550:Spectroscopy, Fourier Transform Infrared; D014416:Tunisia; D017155:Wettability
chemical_list: D060734:Nacre; D012710:Serum Albumin, Bovine; D006046:Gold