title: The surface modification of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) copolymers to improve the attachment of urothelial cells.
abstract: Biocompatible and biodegradable poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) [P(HB-co-HHx)] substrates were modified to improve the attachment of porcine urothelial cell culture. The pristine copolymer exhibits excellent mechanical properties to replace the bladder tissue, but its surface lacks chemical functionalities to interact with cells. Thus, wet chemical treatments based on NaOH and ethylenediamine in aqueous [ED(aq)] and isopropanol [ED(isoOH)] media to functionalize the P(HB-co-HHx) films surfaces were compared. Among these treatments, short ED(aq) treatment was able to decrease the hydrophobicity, rendering a surface with amino groups and without a significant alteration of the mechanical properties. Furthermore, to enhance the interaction with urothelial cells, laminin derived YIGSR sequence was covalently bound to these amino functionalized substrates. The focal attachment was clearly improved with this last treatment, comparing with those results found with the unmodified and first-step functionalized P(HB-co-HHx).
authors.affiliation: Departamento de Química-Física de Polímeros, Instituto de Ciencia y Tecnología de Polímeros, Consejo Superior de Investigaciones Científicas (ICTP-CSIC), Juan de la Cierva, 3, E-28006, Madrid, Spain.
authors.forename: José M
authors.initials: JM
authors.lastname: García-García
authors.affiliation: Departamento de Química-Física de Polímeros, Instituto de Ciencia y Tecnología de Polímeros, Consejo Superior de Investigaciones Científicas (ICTP-CSIC), Juan de la Cierva, 3, E-28006, Madrid, Spain. Electronic address: iquijada@ictp.csic.es.
authors.forename: Isabel
authors.initials: I
authors.lastname: Quijada-Garrido
authors.affiliation: Hospital Universitario Fundación de Alcorcón C/Budapest, 1, E-28922, Alcorcón, Madrid, Spain.
authors.forename: Laura
authors.initials: L
authors.lastname: López
authors.affiliation: Departamento de Química-Física de Polímeros, Instituto de Ciencia y Tecnología de Polímeros, Consejo Superior de Investigaciones Científicas (ICTP-CSIC), Juan de la Cierva, 3, E-28006, Madrid, Spain.
authors.forename: Rodrigo
authors.initials: R
authors.lastname: París
authors.affiliation: Hospital Universitario Fundación de Alcorcón C/Budapest, 1, E-28922, Alcorcón, Madrid, Spain.
authors.forename: María Teresa
authors.initials: MT
authors.lastname: Núñez-López
authors.affiliation: Servicio de Urología del Hospital Universitario Fundación de Alcorcón, Avda. Budapest 1, E-28922, Alcorcón, Spain.
authors.forename: Enrique
authors.initials: E
authors.lastname: de la Peña Zarzuelo
authors.affiliation: Departamento de Química-Física de Polímeros, Instituto de Ciencia y Tecnología de Polímeros, Consejo Superior de Investigaciones Científicas (ICTP-CSIC), Juan de la Cierva, 3, E-28006, Madrid, Spain.
authors.forename: Leoncio
authors.initials: L
authors.lastname: Garrido
mesh_terms: D019840:2-Propanol / Q000737:chemistry; D020155:3-Hydroxybutyric Acid / Q000737:chemistry* / Q000494:pharmacology; D000595:Amino Acid Sequence; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry* / Q000494:pharmacology; D002208:Caproates / Q000737:chemistry* / Q000494:pharmacology; D002448:Cell Adhesion / Q000187:drug effects; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000166:cytology*; D005029:Ethylenediamines / Q000737:chemistry; D057927:Hydrophobic and Hydrophilic Interactions; D010455:Peptides / Q000737:chemistry; D012972:Sodium Hydroxide / Q000737:chemistry; D013499:Surface Properties; D013552:Swine; D013718:Tensile Strength; D000094122:Polyhydroxybutyrates
chemical_list: D019840:2-Propanol; D020155:3-Hydroxybutyric Acid; D001672:Biocompatible Materials; D002208:Caproates; D005029:Ethylenediamines; D010455:Peptides; D012972:Sodium Hydroxide; C115940:poly(3-hydroxybutyrate-co-3-hydroxyhexanoate); D000094122:Polyhydroxybutyrates; C031234:ethylenediamine