title: [Influence of UV-radiation on physical-chemical and functional properties of free immobilized glucoamylase from Aspergillus awamori].
abstract: The effect of UV light (240-390 nm) at the doses of 151-1510 J/m2 on the catalytic activity of free and im- mobilized glucoamylase from Aspergillus awamori was investigated. It was established that the loss of catalytic activity of the enzyme was associated with photochemical transformations of Trp-120, which is part of the active site of glucoamylase. It was shown that the use of collagen as a carrier for immobilization of glucoamylase reduces the constant photoinactivation of the enzyme. It was suggested that the protective effect of collagen in relation to the glucoamylase subjected to UV irradiation may be related to the acceptance of reactive oxygen species by collagen and the formation of the complex carrier-enzyme which is more photostable than the native enzyme.
authors.forename: V G
authors.initials: VG
authors.lastname: Artiukhov
authors.forename: T A
authors.initials: TA
authors.lastname: Kovaleva
authors.forename: E L
authors.initials: EL
authors.lastname: Makarova
authors.forename: O M
authors.initials: OM
authors.lastname: Kozhokina
mesh_terms: D001230:Aspergillus / Q000201:enzymology*; D002384:Catalysis; D004800:Enzymes, Immobilized / Q000528:radiation effects*; D005087:Glucan 1,4-alpha-Glucosidase / Q000737:chemistry / Q000528:radiation effects*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D014466:Ultraviolet Rays
chemical_list: D004800:Enzymes, Immobilized; D005087:Glucan 1,4-alpha-Glucosidase