title: Determination of Functional Activity of Human iPSC-Derived Hepatocytes by Measurement of CYP Metabolism.
abstract: The advent of induced pluripotent stem cell (iPSC) technology has enabled the modeling of an array of specific human disease phenotypes, aiding in the increasingly important and indispensable understanding of disease progression and pathogenesis. Pluripotent stem cell-derived hepatocytes present a new avenue for drug screening and personalized drug testing toward precision medicine. CYP450 microsomal enzymes play a critical role in drug metabolism. Hence, CYP activity measurement of iPSC-derived hepatocytes is a vital prerequisite, to ensure metabolic functionality before proceeding to drug testing. Herein, we describe the protocol for measurement of different CYP450 enzyme activities in human iPSC-derived hepatocytes.
authors.affiliation: Department of Oncology, The Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine, Baltimore, MD, 21205, USA.
authors.forename: Pooja
authors.initials: P
authors.lastname: Chaudhari
authors.affiliation: Department of Oncology, The Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine, Baltimore, MD, 21205, USA.
authors.forename: Neha
authors.initials: N
authors.lastname: Prasad
authors.affiliation: Department of Oncology, The Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine, Baltimore, MD, 21205, USA.
authors.forename: Lipeng
authors.initials: L
authors.lastname: Tian
authors.affiliation: Department of Oncology, The Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine, Baltimore, MD, 21205, USA. yjang3@jhmi.edu.
authors.forename: Yoon-Young
authors.initials: YY
authors.lastname: Jang
mesh_terms: D002448:Cell Adhesion; D018929:Cell Culture Techniques / Q000379:methods; D002454:Cell Differentiation; D002478:Cells, Cultured; D065150:Cellular Reprogramming; D000067470:Cellular Reprogramming Techniques / Q000379:methods; D003470:Culture Media / Q000494:pharmacology; D003577:Cytochrome P-450 Enzyme System / Q000096:biosynthesis* / Q000235:genetics; D015870:Gene Expression; D022781:Hepatocytes / Q000166:cytology / Q000201:enzymology*; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology*; D008163:Luminescent Measurements; D008862:Microsomes, Liver / Q000201:enzymology; D012333:RNA, Messenger / Q000096:biosynthesis; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods
chemical_list: D003470:Culture Media; D012333:RNA, Messenger; D003577:Cytochrome P-450 Enzyme System
keywords: CYP450; Drug metabolism; Hepatic differentiation; Liver function; iPSCs