title: The Effect of Tacrolimus on Reactive Oxygen Species and Total Antioxidant Status in Pancreatic Beta Cell Line.
abstract: OBJECTIVE
  The mechanism responsible for the development of posttransplant diabetes mellitus associated with tacrolimus treatment remains unclear. To investigate the possible effect of tacrolimus on the development of impaired glucose tolerance in transplant recipients, this study focused on early and second phase insulin secretion, which may be affected by reactive oxygen species under tacrolimus therapy.
  
  
  METHODS
  We measured the anti-oxidant status and reactive oxygen species levels before and after tacrolimus treatment. The pro-duction of reactive oxygen species was determined by flow cytometry. Total antioxidant status was measured using total antioxidant status kits.
  
  
  RESULTS
  The antioxidant status decreased while reactive oxygen species level increased significantly (P = .032) CONCLUSIONS: Tacrolimus treatment resulted in dose- and time-dependent increases in the production of reactive oxygen species by beta cells. The antioxidant status decreased in beta cells after tacrolimus treatment. Increased production of reactive oxygen species and decreased antioxidant status by tacrolimus in beta cells may lead to some adverse events.
authors.affiliation: From the Department of Pharmacology, College of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Namvaran
authors.forename: Ali
authors.initials: A
authors.lastname: Sharifi
authors.forename: Mohamad Mehdi
authors.initials: MM
authors.lastname: Namvaran
authors.forename: Neda
authors.initials: N
authors.lastname: Maruf
authors.forename: Negar
authors.initials: N
authors.lastname: Azarpira
mesh_terms: D000975:Antioxidants; D002460:Cell Line; D004305:Dose-Response Relationship, Drug; D006801:Humans; D007166:Immunosuppressive Agents / Q000494:pharmacology*; D007328:Insulin / Q000032:analysis; D050417:Insulin-Secreting Cells / Q000187:drug effects* / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000032:analysis*; D016559:Tacrolimus / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; D007166:Immunosuppressive Agents; D007328:Insulin; D017382:Reactive Oxygen Species; D016559:Tacrolimus