title: Next-generation sequencing of the yellowfin tuna mitochondrial genome reveals novel phylogenetic relationships within the genus Thunnus.
abstract: Recently, the next-generation sequencing (NGS) technology has become a powerful tool for sequencing the teleost mitochondrial genome (mitogenome). Here, we used this technology to determine the mitogenome of the yellowfin tuna (Thunnus albacares). A total of 41,378 reads were generated by Illumina platform with an average depth of 250×. The mitogenome (16,528 bp in length) contained 37 mitochondrial genes with the similar gene order to other typical teleosts. These mitochondrial genes were encoded on the heavy strand except for ND6 and eight tRNA genes. The result of phylogenetic analysis supported two distinct clades dividing the genus Thunnus, but the tuna species of these two genetic clades were different from that of two recognized subgenus based on anatomical characters and geographical distribution. Our results might help to understand the structure, function, and evolutionary history of the yellowfin tuna mitogenome and also provide valuable new insights for phylogenetic affinity of tuna species.
authors.affiliation: a State Key Laboratory of Biocontrol , Institute of Aquatic Economic Animals, Guangdong Province Key Laboratory for Aquatic Economic Animals, School of Life Sciences, Sun Yat-Sen University , Guangzhou , China and.
authors.forename: Liang
authors.initials: L
authors.lastname: Guo
authors.affiliation: a State Key Laboratory of Biocontrol , Institute of Aquatic Economic Animals, Guangdong Province Key Laboratory for Aquatic Economic Animals, School of Life Sciences, Sun Yat-Sen University , Guangzhou , China and.
authors.forename: Mingming
authors.initials: M
authors.lastname: Li
authors.affiliation: b Key Laboratory of East China Sea & Oceanic Fishery Resources Exploitation and Utilization , Ministry of Agriculture, East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences , Shanghai , China.
authors.forename: Heng
authors.initials: H
authors.lastname: Zhang
authors.affiliation: a State Key Laboratory of Biocontrol , Institute of Aquatic Economic Animals, Guangdong Province Key Laboratory for Aquatic Economic Animals, School of Life Sciences, Sun Yat-Sen University , Guangzhou , China and.
authors.forename: Sen
authors.initials: S
authors.lastname: Yang
authors.affiliation: a State Key Laboratory of Biocontrol , Institute of Aquatic Economic Animals, Guangdong Province Key Laboratory for Aquatic Economic Animals, School of Life Sciences, Sun Yat-Sen University , Guangzhou , China and.
authors.forename: Xinghan
authors.initials: X
authors.lastname: Chen
authors.affiliation: a State Key Laboratory of Biocontrol , Institute of Aquatic Economic Animals, Guangdong Province Key Laboratory for Aquatic Economic Animals, School of Life Sciences, Sun Yat-Sen University , Guangzhou , China and.
authors.forename: Zining
authors.initials: Z
authors.lastname: Meng
authors.affiliation: a State Key Laboratory of Biocontrol , Institute of Aquatic Economic Animals, Guangdong Province Key Laboratory for Aquatic Economic Animals, School of Life Sciences, Sun Yat-Sen University , Guangzhou , China and.
authors.forename: Haoran
authors.initials: H
authors.lastname: Lin
mesh_terms: D000818:Animals; D004272:DNA, Mitochondrial / Q000235:genetics; D050259:Genes, Mitochondrial; D054629:Genome, Mitochondrial; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods*; D010802:Phylogeny; D012343:RNA, Transfer / Q000235:genetics; D014413:Tuna / Q000235:genetics*
chemical_list: D004272:DNA, Mitochondrial; D012343:RNA, Transfer
keywords: Mitochondrial genome; phylogeny; thunnus albacares