title: Theoretical and experimental studies on ionic currents in nanopore-based biosensors.
abstract: Novel generation of analytical technology based on nanopores has provided possibilities to fabricate nanofluidic devices for low-cost DNA sequencing or rapid biosensing. In this paper, a simplified model was suggested to describe DNA molecule's translocation through a nanopore, and the internal potential, ion concentration, ionic flowing speed and ionic current in nanopores with different sizes were theoretically calculated and discussed on the basis of Poisson-Boltzmann equation, Navier-Stokes equation and Nernst-Planck equation by considering several important parameters, such as the applied voltage, the thickness and the electric potential distributions in nanopores. In this way, the basic ionic currents, the modulated ionic currents and the current drops induced by translocation were obtained, and the size effects of the nanopores were carefully compared and discussed based on the calculated results and experimental data, which indicated that nanopores with a size of 10 nm or so are more advantageous to achieve high quality ionic current signals in DNA sensing.
authors.affiliation: Suzhou Key Laboratory for Design and Manufacture of Micro-Nano Biomedical Instruments, Suzhou Research Institute of Southeast University, Suzhou 215123, People's Republic of China. liulei@mail.ustc.edu.cn.
authors.forename: Lei
authors.initials: L
authors.lastname: Liu
authors.affiliation: Jiangsu Key Laboratory for Design and Manufacture of Micro-Nano Biomedical Instruments, School of Mechanical Engineering, Southeast University, Nanjing, Jiangsu 211189, People's Republic of China.
authors.forename: Chu
authors.initials: C
authors.lastname: Li
authors.affiliation: Jiangsu Key Laboratory for Design and Manufacture of Micro-Nano Biomedical Instruments, School of Mechanical Engineering, Southeast University, Nanjing, Jiangsu 211189, People's Republic of China.
authors.forename: Jian
authors.initials: J
authors.lastname: Ma
authors.affiliation: Jiangsu Key Laboratory for Design and Manufacture of Micro-Nano Biomedical Instruments, School of Mechanical Engineering, Southeast University, Nanjing, Jiangsu 211189, People's Republic of China.
authors.forename: Yingdong
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Jiangsu Key Laboratory for Design and Manufacture of Micro-Nano Biomedical Instruments, School of Mechanical Engineering, Southeast University, Nanjing, Jiangsu 211189, People's Republic of China.
authors.forename: Zhonghua
authors.initials: Z
authors.lastname: Ni
authors.affiliation: Jiangsu Key Laboratory for Design and Manufacture of Micro-Nano Biomedical Instruments, School of Mechanical Engineering, Southeast University, Nanjing, Jiangsu 211189, People's Republic of China.
authors.forename: Yunfei
authors.initials: Y
authors.lastname: Chen
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation*; D004247:DNA / Q000737:chemistry*; D004563:Electrochemistry; D007477:Ions / Q000737:chemistry*; D008956:Models, Chemical; D058608:Nanopores; D010316:Particle Size
chemical_list: D007477:Ions; D004247:DNA