title: Fabrication of three-dimensional porous scaffold based on collagen fiber and bioglass for bone tissue engineering.
abstract: An ideal scaffold for bone tissue engineering should have interconnected porous structure, good biocompatibility, and mechanical properties well-matched with natural bones. Collagen is the key component in the extracellular matrix (ECM) of natural bones, and plays an important role in bone regeneration. The biological activity of collagen has promoted it to be an advantageous biomaterial for bone tissue engineering; however, the mechanical properties of these scaffolds are insufficient and the porous structures are not stable in the wet state. An effective strategy to solve this problem is to fabricate a hybrid scaffold of biologically derived and synthetic material, which have the necessary bioactivity and mechanical stability needed for bone synthesis. In this work, a three-dimensional macroporous bone scaffold based on collagen (CO) fiber and bioglass (BG) is fabricated by a slurry-dipping technique, and its relevant mechanical and biological properties are evaluated. The CO/BG scaffold is interconnected with a porosity of 81 ± 4.6% and pore size of 40-200 μm. Compared with CO scaffold, water absorption value of CO/BG scaffold decreases greatly from 889% to 52%, which significantly alleviates the swelling behavior of collagen and improves the stability of scaffold structure. The CO/BG scaffold has a compression strength of 5.8 ± 1.6 MPa and an elastic modulus of 0.35 ± 0.01 Gpa, which are well-matched with the mechanical properties of trabecular bones. In vitro cell assays demonstrate that the CO/BG scaffold has good biocompatibility to facilitate the spreading and proliferation of human bone marrow stromal cells. Hence, the CO/BG scaffold is promising for bone tissue engineering application.
authors.affiliation: Shanghai Key Laboratory of Orthopedic Implant, Department of Orthopedic Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200011, China.
authors.forename: Teng
authors.initials: T
authors.lastname: Long
authors.affiliation: The Education Ministry Key Lab of Resource Chemistry and Shanghai Key Laboratory of Rare Earth Functional Materials, Shanghai Normal University, Shanghai, 200234, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Yang
authors.affiliation: Department of Orthopedics and Rehabilitation, Center for Musculoskeletal Research, University of Rochester Medical Center, Rochester, New York, 14642.
authors.forename: Shan-Shan
authors.initials: SS
authors.lastname: Shi
authors.affiliation: The Education Ministry Key Lab of Resource Chemistry and Shanghai Key Laboratory of Rare Earth Functional Materials, Shanghai Normal University, Shanghai, 200234, China.
authors.forename: Ya-Ping
authors.initials: YP
authors.lastname: Guo
authors.affiliation: The Education Ministry Key Lab of Resource Chemistry and Shanghai Key Laboratory of Rare Earth Functional Materials, Shanghai Normal University, Shanghai, 200234, China.
authors.forename: Qin-Fei
authors.initials: QF
authors.lastname: Ke
authors.affiliation: Shanghai Key Laboratory of Orthopedic Implant, Department of Orthopedic Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200011, China.
authors.forename: Zhen-An
authors.initials: ZA
authors.lastname: Zhu
mesh_terms: D001854:Bone Marrow Cells / Q000166:cytology / Q000378:metabolism*; D018786:Bone Substitutes / Q000737:chemistry*; D002516:Ceramics / Q000737:chemistry*; D003094:Collagen / Q000737:chemistry*; D006801:Humans; D008422:Materials Testing; D016062:Porosity; D017154:Stromal Cells / Q000166:cytology / Q000378:metabolism; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: C022107:Bioglass; D018786:Bone Substitutes; D003094:Collagen
keywords: biocompatibility; bioglass; collagen fiber; mechanical property; porous scaffold