title: Electrospun poly(ε-caprolactone)-based skin substitutes: In vivo evaluation of wound healing and the mechanism of cell proliferation.
abstract: In the present study, we have fabricated electrospun poly(ε-caprolactone)-based membranes, characterized and studied the in vivo cell migration and proliferation and wound healing activity. Moreover, we did not seed any cells prior to the animal implantation and we could observe excellent fibroblast attachment and cell proliferation. Further full thickness excision wound on guinea pig completely healed within 35 days. We could reach in an assumption that the enhanced cell proliferation and wound healing might be due to the surface degradation of the polymer under physiological conditions and the formation of functional groups like hydroxyl and carboxyl groups that promoted cell proliferation in a cell adhesion protein mediated mechanism. This study is a novel tissue engineering concept for the reconstruction of a damaged tissue without the in vitro cell seeding and proliferation prior to the in vivo implantation.
authors.affiliation: International and Inter University Centre for Nanoscience and Nanotechnology, Mahatma Gandhi University, Kottayam, 686 560, Kerala, India.
authors.forename: Robin
authors.initials: R
authors.lastname: Augustine
authors.affiliation: Pushpagiri Research Centre, Pushpagiri Institute of Medical Sciences & Research Centre, Tiruvalla, 689 101, Kerala, India.
authors.forename: Edwin Anto
authors.initials: EA
authors.lastname: Dominic
authors.affiliation: Pushpagiri Research Centre, Pushpagiri Institute of Medical Sciences & Research Centre, Tiruvalla, 689 101, Kerala, India.
authors.forename: Indu
authors.initials: I
authors.lastname: Reju
authors.affiliation: Pushpagiri Research Centre, Pushpagiri Institute of Medical Sciences & Research Centre, Tiruvalla, 689 101, Kerala, India.
authors.forename: Balarama
authors.initials: B
authors.lastname: Kaimal
authors.affiliation: International and Inter University Centre for Nanoscience and Nanotechnology, Mahatma Gandhi University, Kottayam, 686 560, Kerala, India.|School of Pure and Applied Physics, Mahatma Gandhi University, Kottayam, 686 560, Kerala, India.
authors.forename: Nandakumar
authors.initials: N
authors.lastname: Kalarikkal
authors.affiliation: International and Inter University Centre for Nanoscience and Nanotechnology, Mahatma Gandhi University, Kottayam, 686 560, Kerala, India.|School of Chemical Sciences, Mahatma Gandhi University, Kottayam, 686 560, Kerala, India.
authors.forename: Sabu
authors.initials: S
authors.lastname: Thomas
mesh_terms: D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects*; D006168:Guinea Pigs; D011091:Polyesters / Q000494:pharmacology*; D012867:Skin / Q000293:injuries* / Q000378:metabolism*; D017768:Skin, Artificial; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D011091:Polyesters; C016240:polycaprolactone
keywords: electrospinning; poly(ε-caprolactone); skin substitutes; wound healing