title: Cryopreservation of transgenic Huntington's disease rhesus macaque sperm-A Case Report.
abstract: The cryoprotective effects of glycerol in three different semen freezing extenders, Tris-citrate (TRIS), TEST, and Tes-Tris-Egg yolk (TTE), on wild-type (WT) rhesus monkey (Macaca mulatta) sperm cryopreservation have been tested. Sperm motility and viability were examined to evaluate the integrity of frozen-thawed sperm, and the best extender was selected to cryopreserve sperm from transgenic Huntington's disease (HD) rhesus monkey. The results showed no post-thaw motility difference among the freezing extender tested (P>0.05). However, sperm membrane integrity in TEST and TTE were significantly better than in TRIS extender (P<0.05). TEST was chosen for HD rhesus monkey sperm cryopreservation. The results showed that post-thawed HD sperm motility and viability was not different compared with WT control group (P>0.05). The present study demonstrates that TEST and TTE were excellent extenders and suitable for rhesus monkey sperm cryopreservation and no detectible differences of post-thaw sperm motility and viability between HD and WT rhesus monkeys resulted from TEST extender.
authors.affiliation: Yerkes National Primate Research Center Emory University School of Medicine 954 Gatewood Rd., N.E. Atlanta, Georgia 30329, USA. ; Embryo Technology and Stem Cell Research Center, School of Biotechnology, Suranaree University of Technology, Nakhon Ratchasima 30000, Thailand.
authors.forename: Kittiphong
authors.initials: K
authors.lastname: Putkhao
authors.affiliation: Department of Veterinary Pathobiology, College of Veterinary Medicine, University of Missouri 1600 East Rollins Rd., Columbia, MO 65211 agcay@missouri.edu.
authors.forename: Anthony W S
authors.initials: AW
authors.lastname: Chan
authors.affiliation: Embryo Technology and Stem Cell Research Center, School of Biotechnology, Suranaree University of Technology, Nakhon Ratchasima 30000, Thailand.
authors.forename: Yuksel
authors.initials: Y
authors.lastname: Agca
authors.affiliation: Yerkes National Primate Research Center Emory University School of Medicine 954 Gatewood Rd., N.E. Atlanta, Georgia 30329, USA. ; Yerkes National Primate Research Center Emory University School of Medicine 954 Gatewood Rd., N.E. Atlanta, Georgia 30329, USA.
authors.forename: Rangsun
authors.initials: R
authors.lastname: Parnpai
keywords: Freezing extender; Huntington's disease; Sperm cryopreservation; Transgenic monkey model