title: Sensitive electrochemical biosensing of H2O2 based on cobalt nanoparticles synthesised in iron storage protein molecules, ferritin.
abstract: In this report, a highly sensitive electrochemical biosensor based on cobaltferritin immobilised on a self-assembled monolayer modiﬁed gold electrode for determination of hydrogen peroxide (H2O2) in phosphate buffer solution (pH 7.5) was investigated. The modiﬁed electrode showed excellent electrochemical activity for oxidation of H2O2. The response to H2O2 on the modiﬁed electrode was examined using linear sweep and differential pulse voltammetries. In phosphate buffer (pH 7.5, 0.1 M), the fabricated biosensor exhibited a linear dependence (R = 0.989) on the concentration of H2O2 from 2.49 × 10(-9) to 1.91 × 10(-8) M, a high sensitivity of -0.4099 µA/nM and detection limit of 2.48 × 10(-9) based on a signal-to-noise ratio of 3. Charge transfer coefﬁcient (α) and the exchange current (i0) of oxidation for H2O2 were found to be 0.57 and 7.55 A, respectively. It has been shown that, this modiﬁed electrode is able to determine H2O2 with a high sensitivity, low detection limit and high selectivity.
authors.affiliation: Faculty of Chemistry, Razi University, Kermanshah, Iran.
authors.forename: Ronak
authors.initials: R
authors.lastname: Rafipour
authors.affiliation: Faculty of Chemistry, Sensor and Biosensor Research Center (SBRC) & Nanoscience and Nanotechnology Research Center (NNRC), Razi University, Kermanshah, Iran. kashanian_s@yahoo.com.
authors.forename: Soheila
authors.initials: S
authors.lastname: Kashanian
authors.affiliation: Department of Biology, Faculty of Science, Razi University, Kermanshah, Iran.
authors.forename: Fereshteh
authors.initials: F
authors.lastname: Abasi Tarighat
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D003035:Cobalt / Q000737:chemistry*; D055664:Electrochemical Techniques; D005293:Ferritins / Q000737:chemistry*; D006861:Hydrogen Peroxide / Q000032:analysis*; D057230:Limit of Detection; D016014:Linear Models; D053768:Metal Nanoparticles / Q000737:chemistry*
chemical_list: D003035:Cobalt; D005293:Ferritins; D006861:Hydrogen Peroxide