title: All-in-one nanowire-decorated multifunctional membrane for rapid cell lysis and direct DNA isolation.
abstract: This paper describes a handheld device that uses an all-in-one membrane for continuous mechanical cell lysis and rapid DNA isolation without the assistance of power sources, lysis reagents, and routine centrifugation. This nanowire-decorated multifunctional membrane was fabricated to isolate DNA by selective adsorption to silica surface immediately after disruption of nucleus membranes by ultrasharp tips of nanowires for a rapid cell lysis, and it can be directly assembled with commercial syringe filter holders. The membrane was fabricated by photoelectrochemical etching to create microchannel arrays followed by hydrothermal synthesis of nanowires and deposition of silica. The proposed membrane successfully purifies high-quality DNA within 5 min, whereas a commercial purification kit needs more than an hour.
authors.affiliation: Department of Mechanical Engineering, Berkeley Sensor & Actuator Center and ‡Department of Bioengineering, University of California , Berkeley, California 94720, United States.
authors.forename: Hongyun
authors.initials: H
authors.lastname: So
authors.forename: Kunwoo
authors.initials: K
authors.lastname: Lee
authors.forename: Niren
authors.initials: N
authors.lastname: Murthy
authors.forename: Albert P
authors.initials: AP
authors.lastname: Pisano
mesh_terms: D000327:Adsorption; D002462:Cell Membrane / Q000737:chemistry*; D004247:DNA / Q000737:chemistry / Q000302:isolation & purification*; D053770:Nanowires / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry*
chemical_list: D012822:Silicon Dioxide; D004247:DNA
keywords: DNA purification; cell lysis; nanowires; point-of-care diagnostics; porous silicon membrane