title: The in vitro biocompatibility and macrophage phagocytosis of Mg17Al12 phase in Mg-Al-Zn alloys.
abstract: Mg alloys are gaining interest for applications as biodegradable medical implant, including Mg-Al-Zn series alloys with good combination of mechanical properties and reasonable corrosion resistance. However, whether the existence of second phase particles in the alloys exerts influence on the biocompatibility is still not clear. A deeper understanding of how the particles regulate specific biological responses is becoming a crucial requirement for their subsequent biomedical application. In this work, the in vitro biocompatibility of Mg17Al12 as a common second phase in biodegradable Mg-Al-Zn alloys was investigated via hemolysis, cytotoxicity, cell proliferation, and cell adhesion tests. Moreover, osteogenic differentiation was evaluated by the extracellular matrix mineralization assay. The Mg17Al12 particles were also prepared to simulate the real situation of second phase in the in vivo environment in order to estimate the cellular response in macrophages to the Mg17Al12 particles. The experimental results indicated that no hemolysis was found and an excellent cytocompatibility was also proved for the Mg17Al12 second phase when co-cultured with L929 cells, MC3T3-E1 cells and BMSCs. Macrophage phagocytosis co-culture test revealed that Mg17Al12 particles exerted no harmful effect on RAW264.7 macrophages and could be phagocytized by the RAW264.7 cells. Furthermore, the possible inflammatory reaction and metabolic way for Mg17Al12 phase were also discussed in detail.
authors.affiliation: Department of Materials Science and Engineering, Nanjing University of Science and Technology, Nanjing, 210094, China.|Institute of Metal Research, Chinese Academy of Science, Shenyang, 110016, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Liu
authors.affiliation: Southern Medical University, Guangzhou, 510515, China.|Hospital of Orthopedics, Guangzhou General Hospital of Guangzhou Military Command, Guangzhou, 510010, China.
authors.forename: Peng
authors.initials: P
authors.lastname: He
authors.affiliation: Institute of Metal Research, Chinese Academy of Science, Shenyang, 110016, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Wan
authors.affiliation: Hospital of Orthopedics, Guangzhou General Hospital of Guangzhou Military Command, Guangzhou, 510010, China.
authors.forename: Mei
authors.initials: M
authors.lastname: Li
authors.affiliation: Department of Materials Science and Engineering, Nanjing University of Science and Technology, Nanjing, 210094, China.
authors.forename: Kehong
authors.initials: K
authors.lastname: Wang
authors.affiliation: Institute of Metal Research, Chinese Academy of Science, Shenyang, 110016, China.
authors.forename: Lili
authors.initials: L
authors.lastname: Tan
authors.affiliation: Hospital of Orthopedics, Guangzhou General Hospital of Guangzhou Military Command, Guangzhou, 510010, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Institute of Metal Research, Chinese Academy of Science, Shenyang, 110016, China.
authors.forename: Ke
authors.initials: K
authors.lastname: Yang
mesh_terms: D000497:Alloys; D000818:Animals; D001672:Biocompatible Materials; D002460:Cell Line; D066298:In Vitro Techniques; D008264:Macrophages / Q000502:physiology*; D051379:Mice; D010587:Phagocytosis
chemical_list: D000497:Alloys; D001672:Biocompatible Materials
keywords: Mg17Al12; biocompatibility; macrophage phagocytosis; magnesium alloys; second phase