title: The effect of gestational diabetes on proliferation capacity and viability of human umbilical cord-derived stromal cells.
abstract: Stomal cells derived from Wharton's jelly of human umbilical cord (WJMSCs) are considered as the potential therapeutic agents for regeneration and are getting famous for stem cell banking. Our study aims to evaluate the effects of gestational diabetes on proliferation capacity and viability of WJMSCs. Mesenchymal stromal cells were isolated from Wharton's jelly of human umbilical cords from normal and gestational diabetic (DWJMSCs) mothers. Growth patterns of both types of cells were analyzed through MTT assay and population doubling time. Cell survival, cell death and glucose utilization were estimated through trypan blue exclusion assay, LDH assay and glucose detection assay respectively. Angiogenic ability was evaluated by immunocytochemistry and ELISA for VEGF A. Anti-cancerous potential was analyzed on HeLa cells. DWJMSCs exhibited low proliferative rate, increased population doubling time, reduced cell viability and increased cell death. Interestingly, DWJMSCs were found to have a reduced glucose utilization and anti-cancerous ability while enhanced angiogenic ability. Gestational diabetes induces adverse effects on growth, angiogenic and anti-cancerous potential of WJMSCs.
authors.affiliation: IMBB, The University of Lahore, Defense Road Campus, Lahore, Pakistan, Nadia.wajid@imbb.uol.edu.pk.
authors.forename: Nadia
authors.initials: N
authors.lastname: Wajid
authors.forename: Rashida
authors.initials: R
authors.lastname: Naseem
authors.forename: Sanam Saiqa
authors.initials: SS
authors.lastname: Anwar
authors.forename: Sana Javaid
authors.initials: SJ
authors.lastname: Awan
authors.forename: Muhammad
authors.initials: M
authors.lastname: Ali
authors.forename: Sara
authors.initials: S
authors.lastname: Javed
authors.forename: Fatima
authors.initials: F
authors.lastname: Ali
mesh_terms: D017209:Apoptosis; D049109:Cell Proliferation; D002470:Cell Survival / Q000502:physiology; D002478:Cells, Cultured; D016640:Diabetes, Gestational / Q000473:pathology* / Q000503:physiopathology*; D005260:Female; D005312:Fetal Blood / Q000166:cytology* / Q000378:metabolism; D005947:Glucose / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism*; D018919:Neovascularization, Physiologic; D011247:Pregnancy
chemical_list: D005947:Glucose