title: Detection panel for identification of twelve hemorrhagic viruses using real-time RT-PCR.
abstract: BACKGROUND
  Viral hemorrhagic fevers are caused by viruses from four viral families and develop diseases with high fatality rates. However, no commercial diagnostic assay for these pathogens is available.
  
  
  RESULTS
  We developed real-time RT-PCR assays for viruses Ebola, Marburg, Lassa, Guanarito, Machupo, Junin, Sabiá, Seoul, Puumala, Hantaan, Crimean-Congo hemorrhagic fever virus and Rift Valley fever virus. The assays were optimized for identical reaction conditions and can be performed using several types of real-time PCR instruments, both capillary and plate, including a portable Ruggedized Advanced Pathogen Identification Device (R.A.P.I.D.) (Idaho Technology, Inc.).
  
  
  CONCLUSIONS
  In combination with primers and probes from previously published studies, we present a simple system for rapid identification of hemorrhagic filoviruses, arenaviruses and bunyaviruses with sufficient sensitivity for first contact laboratory and diagnosis under field conditions.
authors.forename: M
authors.initials: M
authors.lastname: Fajfr
authors.forename: V
authors.initials: V
authors.lastname: Neubauerová
authors.forename: P
authors.initials: P
authors.lastname: Pajer
authors.forename: P
authors.initials: P
authors.lastname: Kubíčková
authors.forename: D
authors.initials: D
authors.lastname: Růžek
mesh_terms: D006482:Hemorrhagic Fevers, Viral / Q000821:virology*; D006801:Humans; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D014780:Viruses / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*