title: Pollen tube cell walls of wild and domesticated tomatoes contain arabinosylated and fucosylated xyloglucan.
abstract: OBJECTIVE
  In flowering plants, fertilization relies on the delivery of the sperm cells carried by the pollen tube to the ovule. During the tip growth of the pollen tube, proper assembly of the cell wall polymers is required to maintain the mechanical properties of the cell wall. Xyloglucan (XyG) is a cell wall polymer known for maintaining the wall integrity and thus allowing cell expansion. In most angiosperms, the XyG of somatic cells is fucosylated, except in the Asterid clade (including the Solanaceae), where the fucosyl residues are replaced by arabinose, presumably due to an adaptive and/or selective diversification. However, it has been shown recently that XyG of Nicotiana alata pollen tubes is mostly fucosylated. The objective of the present work was to determine whether such structural differences between somatic and gametophytic cells are a common feature of Nicotiana and Solanum (more precisely tomato) genera.
  
  
  METHODS
  XyGs of pollen tubes of domesticated (Solanum lycopersicum var. cerasiforme and var. Saint-Pierre) and wild (S. pimpinellifolium and S. peruvianum) tomatoes and tobacco (Nicotiana tabacum) were analysed by immunolabelling, oligosaccharide mass profiling and GC-MS analyses.
  
  
  RESULTS
  Pollen tubes from all the species were labelled with the mAb CCRC-M1, a monoclonal antibody that recognizes epitopes associated with fucosylated XyG motifs. Analyses of the cell wall did not highlight major structural differences between previously studied N. alata and N. tabacum XyG. In contrast, XyG of tomato pollen tubes contained fucosylated and arabinosylated motifs. The highest levels of fucosylated XyG were found in pollen tubes from the wild species.
  
  
  CONCLUSIONS
  The results clearly indicate that the male gametophyte (pollen tube) and the sporophyte have structurally different XyG. This suggests that fucosylated XyG may have an important role in the tip growth of pollen tubes, and that they must have a specific set of functional XyG fucosyltransferases, which are yet to be characterized.
authors.affiliation: Laboratoire de Glycobiologie et Matrice Extracellulaire Végétale (Glyco-MEV), EA 4358, Normandy University, IRIB, VASI, 76821 Mont-Saint-Aignan Cedex, France, COBRA, UMR6014 and FR3038, Normandy University, INSA Rouen, CNRS, IRCOF, 76821 Mont-Saint-Aignan Cedex, France, Laboratoire Polymères, Biopolymères, Surfaces, UMR CNRS 6270, Normandy University, 76821 Mont-Saint-Aignan Cedex, France and Génétique et Amélioration des Fruits et Légumes, INRA UR1052, 84143 Montfavet Cedex, France.
authors.forename: Flavien
authors.initials: F
authors.lastname: Dardelle
authors.affiliation: Laboratoire de Glycobiologie et Matrice Extracellulaire Végétale (Glyco-MEV), EA 4358, Normandy University, IRIB, VASI, 76821 Mont-Saint-Aignan Cedex, France, COBRA, UMR6014 and FR3038, Normandy University, INSA Rouen, CNRS, IRCOF, 76821 Mont-Saint-Aignan Cedex, France, Laboratoire Polymères, Biopolymères, Surfaces, UMR CNRS 6270, Normandy University, 76821 Mont-Saint-Aignan Cedex, France and Génétique et Amélioration des Fruits et Légumes, INRA UR1052, 84143 Montfavet Cedex, France.
authors.forename: François
authors.initials: F
authors.lastname: Le Mauff
authors.affiliation: Laboratoire de Glycobiologie et Matrice Extracellulaire Végétale (Glyco-MEV), EA 4358, Normandy University, IRIB, VASI, 76821 Mont-Saint-Aignan Cedex, France, COBRA, UMR6014 and FR3038, Normandy University, INSA Rouen, CNRS, IRCOF, 76821 Mont-Saint-Aignan Cedex, France, Laboratoire Polymères, Biopolymères, Surfaces, UMR CNRS 6270, Normandy University, 76821 Mont-Saint-Aignan Cedex, France and Génétique et Amélioration des Fruits et Légumes, INRA UR1052, 84143 Montfavet Cedex, France.
authors.forename: Arnaud
authors.initials: A
authors.lastname: Lehner
authors.affiliation: Laboratoire de Glycobiologie et Matrice Extracellulaire Végétale (Glyco-MEV), EA 4358, Normandy University, IRIB, VASI, 76821 Mont-Saint-Aignan Cedex, France, COBRA, UMR6014 and FR3038, Normandy University, INSA Rouen, CNRS, IRCOF, 76821 Mont-Saint-Aignan Cedex, France, Laboratoire Polymères, Biopolymères, Surfaces, UMR CNRS 6270, Normandy University, 76821 Mont-Saint-Aignan Cedex, France and Génétique et Amélioration des Fruits et Légumes, INRA UR1052, 84143 Montfavet Cedex, France.
authors.forename: Corinne
authors.initials: C
authors.lastname: Loutelier-Bourhis
authors.affiliation: Laboratoire de Glycobiologie et Matrice Extracellulaire Végétale (Glyco-MEV), EA 4358, Normandy University, IRIB, VASI, 76821 Mont-Saint-Aignan Cedex, France, COBRA, UMR6014 and FR3038, Normandy University, INSA Rouen, CNRS, IRCOF, 76821 Mont-Saint-Aignan Cedex, France, Laboratoire Polymères, Biopolymères, Surfaces, UMR CNRS 6270, Normandy University, 76821 Mont-Saint-Aignan Cedex, France and Génétique et Amélioration des Fruits et Légumes, INRA UR1052, 84143 Montfavet Cedex, France.
authors.forename: Muriel
authors.initials: M
authors.lastname: Bardor
authors.affiliation: Laboratoire de Glycobiologie et Matrice Extracellulaire Végétale (Glyco-MEV), EA 4358, Normandy University, IRIB, VASI, 76821 Mont-Saint-Aignan Cedex, France, COBRA, UMR6014 and FR3038, Normandy University, INSA Rouen, CNRS, IRCOF, 76821 Mont-Saint-Aignan Cedex, France, Laboratoire Polymères, Biopolymères, Surfaces, UMR CNRS 6270, Normandy University, 76821 Mont-Saint-Aignan Cedex, France and Génétique et Amélioration des Fruits et Légumes, INRA UR1052, 84143 Montfavet Cedex, France.
authors.forename: Christophe
authors.initials: C
authors.lastname: Rihouey
authors.affiliation: Laboratoire de Glycobiologie et Matrice Extracellulaire Végétale (Glyco-MEV), EA 4358, Normandy University, IRIB, VASI, 76821 Mont-Saint-Aignan Cedex, France, COBRA, UMR6014 and FR3038, Normandy University, INSA Rouen, CNRS, IRCOF, 76821 Mont-Saint-Aignan Cedex, France, Laboratoire Polymères, Biopolymères, Surfaces, UMR CNRS 6270, Normandy University, 76821 Mont-Saint-Aignan Cedex, France and Génétique et Amélioration des Fruits et Légumes, INRA UR1052, 84143 Montfavet Cedex, France.
authors.forename: Mathilde
authors.initials: M
authors.lastname: Causse
authors.affiliation: Laboratoire de Glycobiologie et Matrice Extracellulaire Végétale (Glyco-MEV), EA 4358, Normandy University, IRIB, VASI, 76821 Mont-Saint-Aignan Cedex, France, COBRA, UMR6014 and FR3038, Normandy University, INSA Rouen, CNRS, IRCOF, 76821 Mont-Saint-Aignan Cedex, France, Laboratoire Polymères, Biopolymères, Surfaces, UMR CNRS 6270, Normandy University, 76821 Mont-Saint-Aignan Cedex, France and Génétique et Amélioration des Fruits et Légumes, INRA UR1052, 84143 Montfavet Cedex, France.
authors.forename: Patrice
authors.initials: P
authors.lastname: Lerouge
authors.affiliation: Laboratoire de Glycobiologie et Matrice Extracellulaire Végétale (Glyco-MEV), EA 4358, Normandy University, IRIB, VASI, 76821 Mont-Saint-Aignan Cedex, France, COBRA, UMR6014 and FR3038, Normandy University, INSA Rouen, CNRS, IRCOF, 76821 Mont-Saint-Aignan Cedex, France, Laboratoire Polymères, Biopolymères, Surfaces, UMR CNRS 6270, Normandy University, 76821 Mont-Saint-Aignan Cedex, France and Génétique et Amélioration des Fruits et Légumes, INRA UR1052, 84143 Montfavet Cedex, France.
authors.forename: Azeddine
authors.initials: A
authors.lastname: Driouich
authors.affiliation: Laboratoire de Glycobiologie et Matrice Extracellulaire Végétale (Glyco-MEV), EA 4358, Normandy University, IRIB, VASI, 76821 Mont-Saint-Aignan Cedex, France, COBRA, UMR6014 and FR3038, Normandy University, INSA Rouen, CNRS, IRCOF, 76821 Mont-Saint-Aignan Cedex, France, Laboratoire Polymères, Biopolymères, Surfaces, UMR CNRS 6270, Normandy University, 76821 Mont-Saint-Aignan Cedex, France and Génétique et Amélioration des Fruits et Légumes, INRA UR1052, 84143 Montfavet Cedex, France jean-claude.mollet@univ-rouen.fr.
authors.forename: Jean-Claude
authors.initials: JC
authors.lastname: Mollet
mesh_terms: D001089:Arabinose / Q000378:metabolism; D005647:Fucosyltransferases / Q000378:metabolism; D008401:Gas Chromatography-Mass Spectrometry; D005936:Glucans / Q000378:metabolism*; D007150:Immunohistochemistry; D018551:Solanum lycopersicum / Q000201:enzymology / Q000378:metabolism*; D009844:Oligosaccharides / Q000737:chemistry; D010940:Plant Proteins / Q000378:metabolism; D053205:Pollen Tube / Q000378:metabolism; D032322:Solanum / Q000201:enzymology / Q000378:metabolism*; D014026:Nicotiana / Q000201:enzymology / Q000378:metabolism*; D014990:Xylans / Q000378:metabolism*
chemical_list: D005936:Glucans; D009844:Oligosaccharides; D010940:Plant Proteins; D014990:Xylans; C029353:xyloglucan; D001089:Arabinose; D005647:Fucosyltransferases
keywords: GC-MS; Nicotiana tabacum.; OLIMP; S. peruvianum; S. pimpinellifolium; Solanum lycopersicum; XyG; gametophyte; immunolabelling; oligosaccharide mass profiling; plant cell wall; pollen tube; tomato; xyloglucan