title: Canine distemper virus antigen detection in external epithelia of recently vaccinated, sick dogs by fluorescence microscopy is a valuable prognostic indicator.
abstract: Currently, there are no reliable predictors of the clinical outcomes of domesticated dogs that have been recently vaccinated against canine distemper virus (CDV) and develop respiratory disease. In this study, vaccinated dogs from Oklahoma City that were showing clinical signs of respiratory disease were evaluated for CDV antigen using a direct fluorescent antibody test (FAT). Clinical outcomes after standard symptomatic therapy for respiratory disease were recorded, and a statistical analysis of the results was performed. We present our study showing that CDV FAT results were predictive of clinical recovery (prognostic indicator, prospects of clinical recovery) among vaccinated dogs showing clinical signs of respiratory disease. Negative CDV FAT results equated to 80% chances of recovery after symptomatic therapy, compared to 55% chances of recovery when the CDV FAT results were positive. Based on the results of this study, we show that veterinarians can make better informed decisions about the clinical outcomes of suspected CDV cases, with 2-h turnaround times, by using the CDV FAT. Thus, antemortem examination with the CDV FAT on external epithelia of recently vaccinated, sick dogs is a clinically useful diagnostic test and valuable prognostic indicator for veterinarians. Application of the CDV FAT to these samples avoids unnecessary euthanasia of dogs with suspected CDV.
authors.affiliation: Oklahoma Animal Disease Diagnostic Laboratory, Center for Veterinary Health Sciences, Stillwater, Oklahoma, USA sanjay.kapil@okstate.edu.
authors.forename: Sanjay
authors.initials: S
authors.lastname: Kapil
authors.affiliation: Neel Veterinary Clinic, Oklahoma City, Oklahoma, USA.
authors.forename: Tina
authors.initials: T
authors.lastname: Neel
mesh_terms: D000818:Animals; D000956:Antigens, Viral / Q000032:analysis*; D004216:Distemper / Q000175:diagnosis* / Q000821:virology*; D004217:Distemper Virus, Canine / Q000302:isolation & purification*; D004285:Dogs; D004848:Epithelium / Q000821:virology*; D019085:Fluorescent Antibody Technique, Direct / Q000379:methods; D008856:Microscopy, Fluorescence / Q000379:methods*; D009825:Oklahoma; D011379:Prognosis; D012141:Respiratory Tract Infections / Q000175:diagnosis / Q000662:veterinary* / Q000821:virology
chemical_list: D000956:Antigens, Viral