title: Isolation of a new triterpene derivative and in vitro and in vivo anticancer activity of ethanolic extract from root bark of Zizyphus nummularia Aubrev.
abstract: The various parts of Zizyphus nummularia has been used traditionally in several disease conditions. However, its anticancer activity and mechanism of action remain to be elucidated. Considering this, the objective of this study was to isolate, identify and screen for possible anticancer activity in vitro and in vivo of the ethanolic extract (EE) and isolated identified compound (IC) from Z. nummularia root bark. The in vitro activity against human breast cancer, leukaemia, ovarian cancer, colon adenocarcinoma and human kidney carcinoma cell lines was determined. The in vivo activity in female Swiss albino mice against Ehrlich ascites carcinoma (EAC) was determined. The isolated compound is a new triterpene derivative. EE/IC showed cytotoxicity against different cell lines. The administration of EE/IC decreased tumour parameters such as tumour volume, viable tumour cell count and increased body weight, haematological parameters and life span in comparison to the EAC control mice.
authors.affiliation: a Department of Pharmaceutical Technology , Jadavpur University , Kolkata 700 032 , India.
authors.forename: Sarbani Dey
authors.initials: SD
authors.lastname: Ray
authors.forename: Saikat
authors.initials: S
authors.lastname: Dewanjee
mesh_terms: D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry*; D045744:Cell Line, Tumor / Q000187:drug effects; D005260:Female; D006801:Humans; D007928:Lethal Dose 50; D051379:Mice; D015394:Molecular Structure; D010936:Plant Extracts / Q000737:chemistry*; D018517:Plant Roots / Q000737:chemistry; D023382:Toxicity Tests, Acute; D014315:Triterpenes / Q000737:chemistry*; D031957:Ziziphus / Q000737:chemistry*
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D010936:Plant Extracts; D014315:Triterpenes; C000602151:octadecahydro-picene-2,3-14-15-tetranone
keywords: Ehrlich ascites carcinoma; Zizyphus nummularia; anticancer; cell lines; triterpene derivatives