title: Evaluation of the Sagittal Saw Blade as an Intraoperative Fomite During Diabetic Foot Surgery.
abstract: Surgical site infection is a major potential complication of all operative interventions, and the diabetic foot is particularly at risk for bacterial recontamination and infectious sequelae. The objective of this study was to identify whether the sagittal saw blade used during partial foot amputations and diabetic foot debridements carries the potential to serve as a bacterial fomite. We physically cultured the sagittal saw blade during 20 foot debridements involving the resection of bone in patients diagnosed with a diabetic foot infection. The culture was taken after the initial debridement and during the irrigation phase of the procedure. We observed 16 positive routine intraoperative culture results, with positive saw blade culture results in 15 (93.8%; 15/16) of these cases. In 14 (93.3%; 14/15) of these cases, the saw blade culture grew at least one of the same bacteria as our other routine intraoperative cultures. We observed 4 negative routine intraoperative culture results, with negative saw blade culture results in 3 (75.0%; 3/4) of these cases. This results in agreement between routine intraoperative cultures and saw blade culture of 85.0% (17/20). The results of this investigation demonstrate that the sagittal saw blade used for osseous resection during diabetic foot debridements and partial foot amputations carries the potential for intraoperative bacterial transmission. We recommend changing at least the sagittal saw blade if more bone is resected following irrigation, particularly if it is used to obtain a "clean margin" for microbiological or histological examination.
  
  
  METHODS
  Therapeutic, Level IV: Case series.
authors.affiliation: Podiatric Surgical Residency Program, Temple University Hospital, Philadelphia, Pennsylvania (CLC)Podiatric Surgical Residency Program, St Luke's Allentown Hospital, Allentown, Pennsylvania (JM)Department of Podiatric Surgery, Temple University School of Podiatric Medicine, Philadelphia, Pennsylvania (AJM).
authors.forename: Corine L
authors.initials: CL
authors.lastname: Creech
authors.affiliation: Podiatric Surgical Residency Program, Temple University Hospital, Philadelphia, Pennsylvania (CLC)Podiatric Surgical Residency Program, St Luke's Allentown Hospital, Allentown, Pennsylvania (JM)Department of Podiatric Surgery, Temple University School of Podiatric Medicine, Philadelphia, Pennsylvania (AJM).
authors.forename: Jared R
authors.initials: JR
authors.lastname: Malan
authors.affiliation: Podiatric Surgical Residency Program, Temple University Hospital, Philadelphia, Pennsylvania (CLC)Podiatric Surgical Residency Program, St Luke's Allentown Hospital, Allentown, Pennsylvania (JM)Department of Podiatric Surgery, Temple University School of Podiatric Medicine, Philadelphia, Pennsylvania (AJM) ajmeyr@gmail.com.
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: Meyr
mesh_terms: D000671:Amputation, Surgical / Q000295:instrumentation*; D001419:Bacteria / Q000302:isolation & purification*; D017719:Diabetic Foot / Q000601:surgery*; D004866:Equipment Contamination; D050456:Fomites / Q000382:microbiology*; D006801:Humans; D007432:Intraoperative Period; D010019:Osteomyelitis / Q000209:etiology* / Q000382:microbiology; D013525:Surgical Instruments / Q000382:microbiology*; D013530:Surgical Wound Infection / Q000175:diagnosis / Q000209:etiology* / Q000382:microbiology
keywords: bacterial contamination; bone biopsy; diabetic foot infection; microbiological culture; osteomyelitis