title: Impact of basal diet on dextran sodium sulphate (DSS)-induced colitis in rats.
abstract: OBJECTIVE
  Dextran sodium sulphate (DSS)-induced colitis is a widely used model for inflammatory bowel disease. However, various factors including nutrition may affect the development of this colitis. This study aimed to compare and characterize the impact of purified and non-purified basal diets on the development of DSS-induced colitis in the rat.
  
  
  METHODS
  Wistar rats were fed a non-purified or a semi-synthetic purified diet for 21 days. Colitis was then induced in half of the rats by administration of DSS in drinking water (4% w/v) during the last 7 days of experimentation. At the end of the experimental period, colon sections were taken for histopathological examination, determination of various markers of inflammation (myeloperoxidase: MPO, cytokines) and oxidative stress (superoxide dismutase: SOD, catalase: CAT, glutathione peroxidase: GPx and glutathione reductase: GRed activities), and evaluation of the expression of various genes implicated in this disorder.
  
  
  RESULTS
  DSS ingestion induced a more marked colitis in animals receiving the purified diet, as reflected by higher histological score and increased MPO activity. A significant decrease in SOD and CAT activities was also observed in rats fed the purified diet. Also, in these animals, administration of DSS induced a significant increase in interleukin (IL)-1α, IL-1β and IL-6. In addition, various genes implicated in inflammation were over-expressed after ingestion of DSS by rats fed the purified diet.
  
  
  CONCLUSIONS
  These results show that a purified diet promotes the onset of a more severe induced colitis than a non-purified one, highlighting the influence of basal diet in colitis development.
authors.affiliation: Unité de Nutrition Humaine, Equipe ECREIN, Laboratoire de Pharmacognosie et Phytothérapie, Clermont Université, Université d'Auvergne, 63000, Clermont-Ferrand, France.|3inature Biosphère, Parc Naturopôle, 03800, Saint-Bonnet-de-Rochefort, France.
authors.forename: Ahlem
authors.initials: A
authors.lastname: Boussenna
authors.affiliation: Unité de Nutrition Humaine, Equipe ECREIN, Laboratoire de Biochimie Biologie Moléculaire et Nutrition, Clermont Université, Université d'Auvergne, 63000, Clermont-Ferrand, France.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Goncalves-Mendes
authors.affiliation: Service d'Anatomie et de Cytologie Pathologiques, CHU de Clermont-Ferrand, 63003, Clermont-Ferrand, France.
authors.forename: Juliette
authors.initials: J
authors.lastname: Joubert-Zakeyh
authors.affiliation: Biostatistics Unit (DRCI), CHU de Clermont-Ferrand, 63003, Clermont-Ferrand, France.
authors.forename: Bruno
authors.initials: B
authors.lastname: Pereira
authors.affiliation: Unité de Nutrition Humaine, Equipe ECREIN, Laboratoire de Pharmacognosie et Phytothérapie, Clermont Université, Université d'Auvergne, 63000, Clermont-Ferrand, France.
authors.forename: Didier
authors.initials: D
authors.lastname: Fraisse
authors.affiliation: Unité de Nutrition Humaine, Equipe ECREIN, Laboratoire de Biochimie Biologie Moléculaire et Nutrition, Clermont Université, Université d'Auvergne, 63000, Clermont-Ferrand, France.
authors.forename: Marie-Paule
authors.initials: MP
authors.lastname: Vasson
authors.affiliation: Unité de Nutrition Humaine, Equipe ECREIN, Laboratoire de Pharmacognosie et Phytothérapie, Clermont Université, Université d'Auvergne, 63000, Clermont-Ferrand, France.
authors.forename: Odile
authors.initials: O
authors.lastname: Texier
authors.affiliation: Unité de Nutrition Humaine, Equipe ECREIN, Laboratoire de Pharmacognosie et Phytothérapie, Clermont Université, Université d'Auvergne, 63000, Clermont-Ferrand, France. catherine.felgines@udamail.fr.|Laboratoire de Pharmacognosie et Phytothérapie, Faculté de Pharmacie, Clermont Université, Université d'Auvergne, 28 Place Henri-Dunant, BP 38, 63001, Clermont-Ferrand Cedex 1, France. catherine.felgines@udamail.fr.
authors.forename: Catherine
authors.initials: C
authors.lastname: Felgines
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D001835:Body Weight; D002374:Catalase / Q000378:metabolism; D003092:Colitis / Q000139:chemically induced / Q000178:diet therapy*; D003106:Colon / Q000187:drug effects / Q000378:metabolism; D016207:Cytokines / Q000378:metabolism; D016264:Dextran Sulfate / Q000009:adverse effects; D004032:Diet; D004195:Disease Models, Animal; D002149:Energy Intake; D005979:Glutathione Peroxidase / Q000378:metabolism; D005980:Glutathione Reductase / Q000378:metabolism; D007249:Inflammation / Q000139:chemically induced / Q000178:diet therapy; D053582:Interleukin-1alpha / Q000378:metabolism; D053583:Interleukin-1beta / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects; D009195:Peroxidase / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000378:metabolism; D015854:Up-Regulation
chemical_list: D000975:Antioxidants; D016207:Cytokines; D053582:Interleukin-1alpha; D053583:Interleukin-1beta; D015850:Interleukin-6; D016264:Dextran Sulfate; D002374:Catalase; D009195:Peroxidase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005980:Glutathione Reductase
keywords: Colitis; Dextran sodium sulphate; Oxidative stress; Purified diet; Rat