title: A plasmonic nanosensor for immunoassay via enzyme-triggered click chemistry.
abstract: Current techniques for plasmonic immunoassay often require the introduction and additional conjugation of enzyme, and thus cannot accommodate conventional immunoassay platforms. Herein, we develop a plasmonic nanosensor that well accommodates conventional immunoassays and dramatically improves their sensitivity and stability. This plasmonic nanosensor directly employs alkaline phosphatase-triggered click chemistry between azide/alkyne functionalized gold nanoparticles as the readout. This straightforward approach broadens the applicability of nanoparticle-based immunoassays and has great potential for applications in resource-constrained settings.
authors.affiliation: Beijing Engineering Research Center for BioNanotechnology & Key Laboratory for Biological Effects of Nanomaterials and Nanosafety, National Center for NanoScience and Technology , Beijing 100190, China.
authors.forename: Yunlei
authors.initials: Y
authors.lastname: Xianyu
authors.forename: Zhuo
authors.initials: Z
authors.lastname: Wang
authors.forename: Xingyu
authors.initials: X
authors.lastname: Jiang
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism*; D000480:Alkynes / Q000737:chemistry; D001386:Azides / Q000737:chemistry; D015374:Biosensing Techniques / Q000379:methods*; D057930:Click Chemistry; D006046:Gold / Q000737:chemistry; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D007074:Immunoglobulin G / Q000276:immunology; D053768:Metal Nanoparticles / Q000737:chemistry; D036103:Nanotechnology / Q000379:methods*
chemical_list: D000480:Alkynes; D001386:Azides; D007074:Immunoglobulin G; D006046:Gold; D000469:Alkaline Phosphatase
keywords: alkaline phosphatase; click chemistry; gold nanoparticles; naked-eye readout; plasmonic immunoassay