title: Oscillatory AAA+ ATPase Knk1 constitutes a novel morphogenetic pathway in fission yeast.
abstract: Cellular morphogenesis relies partly on cell polarization by the cytoskeleton. In the fission yeast Schizosaccharomyces pombe, it is well established that microtubules (MTs) deliver the spatial cue Tea1, a kelch repeat protein, to the tip regions to direct the growth machinery at the cell tips driving the linear extension of the rod-shaped organism to maintain a straight long axis. Here, we report the characterization of Knk1 (kink), a previously unidentified member of the superfamily of ATPases associated with various cellular activities (AAA(+)), whose deletion causes a unique morphological defect characterized by the formation of kinks close to cell tips. Through genetic analysis, we place Knk1 into a novel pathway controlling cell shape independently of MTs and Tea1. Knk1 localizes at cell tips. Its localization is mediated by the Knk1 N terminus and is enhanced upon ATP binding to the C-terminal ATPase domain. Furthermore, Knk1 tip recruitment is regulated by SRC-like adaptor 2 (Sla2) and cell division cycle 42 (Cdc42) independently of Sla2's role in endocytosis. Finally, we discovered that Knk1 shows an anticorrelated oscillatory behavior between the two cell tips at a periodicity that is different from the reported oscillatory Cdc42 dynamics.
authors.affiliation: Centre de Recherche, Institut Curie, F-75248 Paris, France; CNRS-UMR144, F-75248 Paris, France;
authors.forename: Kathleen
authors.initials: K
authors.lastname: Scheffler
authors.affiliation: Institut de Biologie du Développement de Marseille, CNRS-UMR7288, 13009 Marseille, France; and.
authors.forename: Pierre
authors.initials: P
authors.lastname: Recouvreux
authors.affiliation: Centre de Recherche, Institut Curie, F-75248 Paris, France; CNRS-UMR144, F-75248 Paris, France;
authors.forename: Anne
authors.initials: A
authors.lastname: Paoletti
authors.affiliation: Centre de Recherche, Institut Curie, F-75248 Paris, France; CNRS-UMR144, F-75248 Paris, France; Department of Cell and Developmental Biology, University of Pennsylvania, Philadelphia PA 19104 phong.tran@curie.fr.
authors.forename: Phong T
authors.initials: PT
authors.lastname: Tran
mesh_terms: D000251:Adenosine Triphosphatases / Q000235:genetics* / Q000378:metabolism*; D001683:Biological Clocks / Q000235:genetics; D015153:Blotting, Western; D019295:Computational Biology; D008856:Microscopy, Fluorescence; D008870:Microtubules / Q000378:metabolism; D009024:Morphogenesis / Q000235:genetics* / Q000502:physiology; D012568:Schizosaccharomyces / Q000201:enzymology* / Q000254:growth & development; D029702:Schizosaccharomyces pombe Proteins / Q000235:genetics* / Q000378:metabolism*; D015398:Signal Transduction / Q000235:genetics*
chemical_list: D029702:Schizosaccharomyces pombe Proteins; D000251:Adenosine Triphosphatases; C000597342:Knk1 protein, S pombe
keywords: kink; morphogenesis; oscillation