title: High-fat diet-induced met-hemoglobin formation in rats prone (WOKW) or resistant (DA) to the metabolic syndrome: effect of CoQ10 supplementation.
abstract: The aim of this study was to evaluate the effects of a high-fat diet (HFD) on oxidative indexes in WistarOttawaKarlsburg W (WOKW) rats used as a model of metabolic syndrome in comparison with Dark Agouti (DA) rats used as a control strain. This syndrome is defined by the occurrence of two or more risk factors including obesity, hypertension, dyslipidemia, and insulin resistance. Forty rats were used in the study and the effect of HFD was evaluated in terms of body weight and both hemoglobin and CoQ oxidative status. Moreover, 16 rats (8 of each strain) were supplemented with 3 mg/100 g b.w. of CoQ10 for 1 month in view of its beneficial properties in cardiovascular disease due to its antioxidant activity in the lipid environment. HFD promoted an increase in body weight, in particular in WOKW males, and in the methemoglobin (met-Hb) index in both strains. Moreover, HFD promoted endogenous CoQ10 oxidation. CoQ10 supplementation was able to efficiently counteract the HFD pro-oxidant effects, preventing met-Hb formation and CoQ oxidation.
authors.affiliation: Department of Clinical and Dental Sciences, Polytechnic University of Marche, Ancona, Italy.
authors.forename: Patrick
authors.initials: P
authors.lastname: Orlando
authors.forename: Sonia
authors.initials: S
authors.lastname: Silvestri
authors.forename: Francesca
authors.initials: F
authors.lastname: Brugè
authors.forename: Luca
authors.initials: L
authors.lastname: Tiano
authors.forename: Ingrid
authors.initials: I
authors.lastname: Kloting
authors.forename: Giancarlo
authors.initials: G
authors.lastname: Falcioni
authors.forename: Carlo
authors.initials: C
authors.lastname: Polidori
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage* / Q000378:metabolism; D001835:Body Weight / Q000187:drug effects; D059305:Diet, High-Fat; D004041:Dietary Fats / Q000009:adverse effects*; D019587:Dietary Supplements; D004198:Disease Susceptibility; D005260:Female; D008297:Male; D024821:Metabolic Syndrome / Q000097:blood / Q000178:diet therapy* / Q000209:etiology* / Q000473:pathology; D008706:Methemoglobin / Q000037:antagonists & inhibitors / Q000378:metabolism; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D051381:Rats; D013045:Species Specificity; D016896:Treatment Outcome; D014451:Ubiquinone / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000097:blood
chemical_list: D000975:Antioxidants; D004041:Dietary Fats; D014451:Ubiquinone; D008706:Methemoglobin; C024989:coenzyme Q10
keywords: Dark Agouti; WistarOttawaKarlsburg W; coenzyme Q10; hemoglobin; high-fat diet; metabolic syndrome; methemoglobin; oxidative stress