title: Serotonin receptor 3A controls interneuron migration into the neocortex.
abstract: Neuronal excitability has been shown to control the migration and cortical integration of reelin-expressing cortical interneurons (INs) arising from the caudal ganglionic eminence (CGE), supporting the possibility that neurotransmitters could regulate this process. Here we show that the ionotropic serotonin receptor 3A (5-HT(3A)R) is specifically expressed in CGE-derived migrating interneurons and upregulated while they invade the developing cortex. Functional investigations using calcium imaging, electrophysiological recordings and migration assays indicate that CGE-derived INs increase their response to 5-HT(3A)R activation during the late phase of cortical plate invasion. Using genetic loss-of-function approaches and in vivo grafts, we further demonstrate that the 5-HT(3A)R is cell autonomously required for the migration and proper positioning of reelin-expressing CGE-derived INs in the neocortex. Our findings reveal a requirement for a serotonin receptor in controlling the migration and laminar positioning of a specific subtype of cortical IN.
authors.affiliation: 1] Department of Mental Health and Psychiatry, University of Geneva Medical School, CH-1211 Geneva 4, Switzerland [2] Department of Basic Neurosciences, University of Geneva Medical School, CH-1211 Geneva 4, Switzerland.
authors.forename: Sahana
authors.initials: S
authors.lastname: Murthy
authors.affiliation: 1] Department of Mental Health and Psychiatry, University of Geneva Medical School, CH-1211 Geneva 4, Switzerland [2] Department of Basic Neurosciences, University of Geneva Medical School, CH-1211 Geneva 4, Switzerland.
authors.forename: Mathieu
authors.initials: M
authors.lastname: Niquille
authors.affiliation: 1] Department of Mental Health and Psychiatry, University of Geneva Medical School, CH-1211 Geneva 4, Switzerland [2] Department of Basic Neurosciences, University of Geneva Medical School, CH-1211 Geneva 4, Switzerland.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Hurni
authors.affiliation: 1] Department of Mental Health and Psychiatry, University of Geneva Medical School, CH-1211 Geneva 4, Switzerland [2] Department of Basic Neurosciences, University of Geneva Medical School, CH-1211 Geneva 4, Switzerland.
authors.forename: Greta
authors.initials: G
authors.lastname: Limoni
authors.affiliation: 1] Department of Mental Health and Psychiatry, University of Geneva Medical School, CH-1211 Geneva 4, Switzerland [2] Department of Basic Neurosciences, University of Geneva Medical School, CH-1211 Geneva 4, Switzerland.
authors.forename: Sarah
authors.initials: S
authors.lastname: Frazer
authors.affiliation: Swammerdam Institute for Life Sciences, Center for NeuroScience, University of Amsterdam, Sciencepark 904, 1098 XH Amsterdam, The Netherlands.
authors.forename: Pascal
authors.initials: P
authors.lastname: Chameau
authors.affiliation: Swammerdam Institute for Life Sciences, Center for NeuroScience, University of Amsterdam, Sciencepark 904, 1098 XH Amsterdam, The Netherlands.
authors.forename: Johannes A
authors.initials: JA
authors.lastname: van Hooft
authors.affiliation: CNRS-UMR 8249, Brain Plasticity Unit, ESPCI ParisTech, 10 rue Vauquelin, 75005 Paris, France.
authors.forename: Tania
authors.initials: T
authors.lastname: Vitalis
authors.affiliation: 1] Department of Mental Health and Psychiatry, University of Geneva Medical School, CH-1211 Geneva 4, Switzerland [2] Department of Basic Neurosciences, University of Geneva Medical School, CH-1211 Geneva 4, Switzerland.
authors.forename: Alexandre
authors.initials: A
authors.lastname: Dayer
mesh_terms: D000818:Animals; D015816:Cell Adhesion Molecules, Neuronal / Q000378:metabolism; D002465:Cell Movement / Q000235:genetics*; D016326:Extracellular Matrix Proteins / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental / Q000235:genetics*; D007395:Interneurons / Q000378:metabolism*; D051379:Mice; D019579:Neocortex / Q000196:embryology / Q000378:metabolism*; D009419:Nerve Tissue Proteins / Q000378:metabolism; D044406:Receptors, Serotonin, 5-HT3 / Q000235:genetics*; D000091042:Reelin Protein; D012697:Serine Endopeptidases / Q000378:metabolism
chemical_list: D015816:Cell Adhesion Molecules, Neuronal; D016326:Extracellular Matrix Proteins; C511230:Htr3a protein, mouse; D009419:Nerve Tissue Proteins; D044406:Receptors, Serotonin, 5-HT3; D000091042:Reelin Protein; C000717128:Reln protein, mouse; D012697:Serine Endopeptidases