title: Relationship between apoptosis imaging and radioiodine therapy in tumor cells with different sodium iodide symporter gene expression.
abstract: The therapeutic efficacy of radioiodine (¹³¹I) therapy has been reported to be variable among cancer patients and even between metastatic regions in the same patients. Because the expression level of sodium iodide symporter (NIS) cannot reflect the efficacy of therapy, other strategies are required to predict the precise therapeutic effect of ¹³¹I therapy. In this research, we investigated the correlation between iodine (I) uptake, apoptosis imaging, and therapeutic efficacy. Two HT29 cell lines, cytomegalovirus (CMV)-NIS (or NIS+++) and TERT-NIS (or NIS+), were established by retroviral transfection. I uptake was estimated by I-uptake assay and gamma camera imaging. Apoptosis was evaluated by confocal microscopy and a Maestro fluorescence imaging system (CRi Inc., Woburn, MA) using ApoFlamma (BioACTs, Seoul, Korea), a fluorescent dye-conjugated apoptosis-targeting peptide 1 (ApoPep-1). Therapeutic efficacy was determined by tumor size. The CMV-NIS showed higher I uptake and ApoFlamma signals than TERT-NIS. In xenograft models, CMV-NIS also showed high 99m technetium signals and ApoFlamma signals. Tumor reduction had a stronger correlation with apoptosis imaging signals than with gamma camera imaging signals, which reflect I uptake. Higher NIS-expressing tumors showed increased apoptosis and I uptake, resulting in a significant tumor reduction. Moreover, tumor reduction showed a strong correlation with ApoFlamma imaging compared to I-uptake imaging.
authors.forename: Kyung Oh
authors.initials: KO
authors.lastname: Jung
authors.forename: Hyewon
authors.initials: H
authors.lastname: Youn
authors.forename: Young-Hwa
authors.initials: YH
authors.lastname: Kim
authors.forename: Seunghoo
authors.initials: S
authors.lastname: Kim
authors.forename: Juri
authors.initials: J
authors.lastname: Na
authors.forename: Yong-il
authors.initials: YI
authors.lastname: Kim
authors.forename: Jin Woo
authors.initials: JW
authors.lastname: Park
authors.forename: Keon Wook
authors.initials: KW
authors.lastname: Kang
authors.forename: Dong Soo
authors.initials: DS
authors.lastname: Lee
authors.forename: June-Key
authors.initials: JK
authors.lastname: Chung
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D003110:Colonic Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology / Q000532:radiotherapy*; D015316:Genetic Therapy; D005822:Genetic Vectors / Q000008:administration & dosage; D019073:HT29 Cells; D006801:Humans; D007457:Iodine Radioisotopes / Q000493:pharmacokinetics* / Q000627:therapeutic use; D018613:Microscopy, Confocal; D009842:Oligopeptides / Q000737:chemistry / Q000494:pharmacology*; D061848:Optical Imaging; D012190:Retroviridae / Q000235:genetics; D027981:Symporters / Q000235:genetics / Q000378:metabolism*; D013667:Technetium / Q000494:pharmacology*; D016896:Treatment Outcome; D000099209:Sodium-Iodide Symporters
chemical_list: D007457:Iodine Radioisotopes; D009842:Oligopeptides; D027981:Symporters; D013667:Technetium; D000099209:Sodium-Iodide Symporters; C578342:cysteinyl-glutaminyl-arginyl-prolyl-prolyl-arginine