title: Coffee (Coffea arabica L.).
abstract: Coffee (Coffea sp.) is a perennial plant widely cultivated in many tropical countries. It is a cash crop for millions of small farmers in these areas. As for other tree species, coffee has long breeding cycles, which makes conventional breeding programs time-consuming. For that matter, genetic transformation can be an effective way to introduce a desired trait in elite varieties or for functional genomics. In this chapter, we describe two highly efficient and reliable Agrobacterium-mediated transformation techniques developed for the C. arabica cultivated species: (1) A. tumefaciens to study and introduce genes conferring resistance/tolerance to biotic (coffee leaf rust, insects) and abiotic stress (drought, heat, seed desiccation) in fully transformed plants and (2) A. rhizogenes to study candidate gene expression for nematode resistance in transformed roots.
authors.affiliation: CIRAD, Centre de Coopération Internationale en Recherche Agronomique pour le Développement, UMR RPB, 911 Avenue Agropolis, BP 64501, 34398, Montpellier, France.
authors.forename: Eveline
authors.initials: E
authors.lastname: Déchamp
authors.forename: Jean-Christophe
authors.initials: JC
authors.lastname: Breitler
authors.forename: Thierry
authors.initials: T
authors.lastname: Leroy
authors.forename: Hervé
authors.initials: H
authors.lastname: Etienne
mesh_terms: D016960:Agrobacterium tumefaciens / Q000235:genetics / Q000254:growth & development; D018920:Coculture Techniques; D040503:Coffea / Q000235:genetics* / Q000254:growth & development* / Q000502:physiology; D005818:Genetic Engineering / Q000379:methods*; D018515:Plant Leaves / Q000254:growth & development; D018517:Plant Roots / Q000254:growth & development; D030821:Plants, Genetically Modified; D012038:Regeneration; D012639:Seeds / Q000254:growth & development; D013242:Sterilization; D014170:Transformation, Genetic