title: HLA-F polymorphisms in a Euro-Brazilian population from Southern Brazil.
abstract: HLA-F is a non-classical major histocompatibility complex (MHC) gene. It codes class Ib MHC molecules with restricted distribution and less nucleotide variations than MHC class Ia genes. Of the 22 alleles registered on the IMGT database only four alleles encode for proteins that differ in their primary structure. To estimate genotype and allele frequencies, this study targeted on known protein coding regions of the HLA-F gene. Genotyping was performed by Sequence Base Typing (SBT). The sample was composed by 199-unrelated bone marrow donors from the Brazilian Bone Marrow Donor Registry (REDOME), Euro-Brazilians, from Southern Brazil. About 1673 bp were analyzed. The most frequent allele was HLA-F*01:01 (87.19%), followed by HLA-F*01:03 (12.31%), HLA-F*01:02 (0.25%) and HLA-F*01:04 (0.25%). Significant linkage disequilibrium (LD) was verified between HLA-F and HLA classes I and II alleles. This is the first study regarding HLA-F polymorphisms in a Euro-Brazilian population contributing to the Southern Brazilian genetic characterization.
authors.affiliation: Laboratory of Immunogenetics and Histocompatibility, Genetics Department, Universidade Federal do Paraná, Curitiba, Brazil.
authors.forename: L F S
authors.initials: LF
authors.lastname: Manvailer
authors.forename: P F
authors.initials: PF
authors.lastname: Wowk
authors.forename: S B
authors.initials: SB
authors.lastname: Mattar
authors.forename: J S
authors.initials: JS
authors.lastname: da Siva
authors.forename: M
authors.initials: M
authors.lastname: da Graça Bicalho
authors.forename: V M M S
authors.initials: VM
authors.lastname: Roxo
mesh_terms: D000483:Alleles; D001938:Brazil; D030561:Databases, Nucleic Acid; D005260:Female; D005838:Genotype; D015395:Histocompatibility Antigens Class I / Q000235:genetics*; D006801:Humans; D015810:Linkage Disequilibrium; D008297:Male; D011110:Polymorphism, Genetic
chemical_list: C062341:HLA-F antigens; D015395:Histocompatibility Antigens Class I
keywords: Euro-Brazilians; HLA-F; linkage disequilibrium; polymorphisms; population