title: Evaluation of serum antioxidant activity in patients with liver pathology by the chemiluminescent method.
abstract: Total antioxidant activity of the serum in patients with liver pathology was assessed by two chemiluminescent methods based on different models of free radical oxidation: Hb-H2O2-luminol and ABAP-luminol. Comparative analysis showed a significant, but not high correlation of the results (r=0.798), which can be explained by different mechanisms of induction of free radicals and effects of various serum components (proteins and bilirubin) on the initiation process. The influence of aphysiological concentration of analyzed values manifested in the Hb-H2O2 model. Disagreement between the results of measurements was more pronounced in patients with abnormally high serum bilirubin content. The results suggested that ABAP-luminol chemiluminescent model is more preferable for evaluation of antioxidant activity in clinical practice.
authors.affiliation: Laboratory of Clinical Biochemistry of Lipoproteins, Research-and-Production Russian Cardiological Center, the Ministry of Health of the Russian Federation, Moscow, Russia.
authors.forename: V N
authors.initials: VN
authors.lastname: Titov
authors.forename: N N
authors.initials: NN
authors.lastname: Sazhina
mesh_terms: D000578:Amidines; D000975:Antioxidants / Q000378:metabolism*; D005609:Free Radicals / Q000378:metabolism; D006801:Humans; D006861:Hydrogen Peroxide; D007700:Kinetics; D008107:Liver Diseases / Q000097:blood*; D008163:Luminescent Measurements / Q000379:methods*; D008165:Luminol; D008954:Models, Biological; D010084:Oxidation-Reduction; D044967:Serum / Q000378:metabolism*
chemical_list: D000578:Amidines; D000975:Antioxidants; D005609:Free Radicals; D008165:Luminol; C046728:2,2'-azobis(2-amidinopropane); D006861:Hydrogen Peroxide