title: Genomic analysis using Affymetrix standard microarray genechips (169 format) in degenerate murine retina.
abstract: Microarray is one of the most useful tools for gene expression profiling. The growing development of microarray genechip technology has enabled increasingly sophisticated studies on the differences in gene transcription between diseased and non-diseased tissues and provides clues to their contribution to the disease in a single experiment. Thus, microarray is used in not only in clinical diagnostics, but also to understand pathological processes and identify leads for new treatments. Here, we present a detailed protocol for performing genomic analysis of retinal tissue with Affymetrix genechip microarray together with additional guidelines from the authors.
authors.affiliation: Macular Research Group, Clinical Ophthalmology and Eye Health, Sydney Eye Hospital, The University of Sydney, Level 2 South Block, Hospital Road, Sydney, NSW, 2000, Australia, sook.chung@sydney.edu.au.
authors.forename: Sook Hyun
authors.initials: SH
authors.lastname: Chung
authors.forename: Weiyong
authors.initials: W
authors.lastname: Shen
authors.forename: Mark
authors.initials: M
authors.lastname: Gillies
mesh_terms: D000818:Animals; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000235:genetics; D023281:Genomics; D051379:Mice; D046228:Microarray Analysis / Q000379:methods*; D008967:Molecular Biology / Q000379:methods; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D012160:Retina / Q000378:metabolism* / Q000473:pathology