title: Food allergy enhances allergic asthma in mice.
abstract: BACKGROUND
  Atopic march refers to the typical transition from a food allergy in early childhood to allergic asthma in older children and adults. However the precise interplay of events involving gut, skin and pulmonary inflammation in this process is not completely understood.
  
  
  OBJECTIVE
  To develop a mouse model of mixed food and respiratory allergy mimicking the atopic march and better understand the impact of food allergies on asthma.
  
  
  METHODS
  Food allergy to ovalbumin (OVA) was induced through intra-peritoneal sensitization and intra-gastric challenge, and/or a respiratory allergy to house dust mite (HDM) was obtained through percutaneous sensitization and intra-nasal challenges with dermatophagoides farinae (Der f) extract. Digestive, respiratory and systemic parameters were analyzed.
  
  
  RESULTS
  OVA-mediated gut allergy was associated with an increase in jejunum permeability, and a worsening of Der f-induced asthma with stronger airway hyperresponsiveness and pulmonary cell infiltration, notably eosinophils. There was overproduction of the pro-eosinophil chemokine RANTES in broncho-alveolar lavages associated with an enhanced Th2 cytokine secretion and increased total and Der f-specific IgE when the two allergies were present. Both AHR and lung inflammation increased after a second pulmonary challenge.
  
  
  CONCLUSIONS
  Gut sensitization to OVA amplifies Der f-induced asthma in mice.
authors.affiliation: INSERM U1087, l'institut du Thorax CHU Nantes, Hôpital Laënnec, 44093, Nantes Cedex 1, France. Tiphaine.BIHOUEE@chu-nantes.fr.|CNRS, UMR 6291, Nantes, F-44000, France. Tiphaine.BIHOUEE@chu-nantes.fr.|Université de Nantes, Nantes, F-44000, France. Tiphaine.BIHOUEE@chu-nantes.fr.|DHU2020 médecine personnalisée des maladies chroniques, Nantes, F-44000, France. Tiphaine.BIHOUEE@chu-nantes.fr.|CHU de Nantes, Service de Pédiatrie, Nantes, F-44000, France. Tiphaine.BIHOUEE@chu-nantes.fr.
authors.forename: Tiphaine
authors.initials: T
authors.lastname: Bihouée
authors.affiliation: INSERM U1087, l'institut du Thorax CHU Nantes, Hôpital Laënnec, 44093, Nantes Cedex 1, France. gregory.bouchaud@nantes.inra.fr.|CNRS, UMR 6291, Nantes, F-44000, France. gregory.bouchaud@nantes.inra.fr.|Université de Nantes, Nantes, F-44000, France. gregory.bouchaud@nantes.inra.fr.|DHU2020 médecine personnalisée des maladies chroniques, Nantes, F-44000, France. gregory.bouchaud@nantes.inra.fr.|INRA, UR1268 BIA, Nantes, F-44316, France. gregory.bouchaud@nantes.inra.fr.
authors.forename: Gregory
authors.initials: G
authors.lastname: Bouchaud
authors.affiliation: INSERM U1087, l'institut du Thorax CHU Nantes, Hôpital Laënnec, 44093, Nantes Cedex 1, France. julie.chesne@univ-nantes.fr.|CNRS, UMR 6291, Nantes, F-44000, France. julie.chesne@univ-nantes.fr.|Université de Nantes, Nantes, F-44000, France. julie.chesne@univ-nantes.fr.|DHU2020 médecine personnalisée des maladies chroniques, Nantes, F-44000, France. julie.chesne@univ-nantes.fr.
authors.forename: Julie
authors.initials: J
authors.lastname: Chesné
authors.affiliation: INSERM U1087, l'institut du Thorax CHU Nantes, Hôpital Laënnec, 44093, Nantes Cedex 1, France. david.Lair@inserm.fr.|CNRS, UMR 6291, Nantes, F-44000, France. david.Lair@inserm.fr.|Université de Nantes, Nantes, F-44000, France. david.Lair@inserm.fr.|DHU2020 médecine personnalisée des maladies chroniques, Nantes, F-44000, France. david.Lair@inserm.fr.
authors.forename: David
authors.initials: D
authors.lastname: Lair
authors.affiliation: INSERM U1087, l'institut du Thorax CHU Nantes, Hôpital Laënnec, 44093, Nantes Cedex 1, France. camille.rolland-debord@univ-nantes.fr.|CNRS, UMR 6291, Nantes, F-44000, France. camille.rolland-debord@univ-nantes.fr.|Université de Nantes, Nantes, F-44000, France. camille.rolland-debord@univ-nantes.fr.|DHU2020 médecine personnalisée des maladies chroniques, Nantes, F-44000, France. camille.rolland-debord@univ-nantes.fr.
authors.forename: Camille
authors.initials: C
authors.lastname: Rolland-Debord
authors.affiliation: INSERM U1087, l'institut du Thorax CHU Nantes, Hôpital Laënnec, 44093, Nantes Cedex 1, France. faouzi.braza@etu.univ-nantes.fr.|CNRS, UMR 6291, Nantes, F-44000, France. faouzi.braza@etu.univ-nantes.fr.|Université de Nantes, Nantes, F-44000, France. faouzi.braza@etu.univ-nantes.fr.|DHU2020 médecine personnalisée des maladies chroniques, Nantes, F-44000, France. faouzi.braza@etu.univ-nantes.fr.
authors.forename: Faouzi
authors.initials: F
authors.lastname: Braza
authors.affiliation: INSERM U1087, l'institut du Thorax CHU Nantes, Hôpital Laënnec, 44093, Nantes Cedex 1, France. Marie-Aude.Muller@univ-nantes.fr.|CNRS, UMR 6291, Nantes, F-44000, France. Marie-Aude.Muller@univ-nantes.fr.|Université de Nantes, Nantes, F-44000, France. Marie-Aude.Muller@univ-nantes.fr.|DHU2020 médecine personnalisée des maladies chroniques, Nantes, F-44000, France. Marie-Aude.Muller@univ-nantes.fr.
authors.forename: Marie-Aude
authors.initials: MA
authors.lastname: Cheminant
authors.affiliation: Université de Nantes, Nantes, F-44000, France. philippe.aubert@univ-nantes.fr.|DHU2020 médecine personnalisée des maladies chroniques, Nantes, F-44000, France. philippe.aubert@univ-nantes.fr.|INSERM, UMR U913, Nantes, F-44000, France. philippe.aubert@univ-nantes.fr.
authors.forename: Philippe
authors.initials: P
authors.lastname: Aubert
authors.affiliation: INSERM U1087, l'institut du Thorax CHU Nantes, Hôpital Laënnec, 44093, Nantes Cedex 1, France. guillaume.mahay@free.fr.|CNRS, UMR 6291, Nantes, F-44000, France. guillaume.mahay@free.fr.|Université de Nantes, Nantes, F-44000, France. guillaume.mahay@free.fr.|DHU2020 médecine personnalisée des maladies chroniques, Nantes, F-44000, France. guillaume.mahay@free.fr.
authors.forename: Guillaume
authors.initials: G
authors.lastname: Mahay
authors.affiliation: INSERM U1087, l'institut du Thorax CHU Nantes, Hôpital Laënnec, 44093, Nantes Cedex 1, France. christine.sagan@chu-nantes.fr.|CNRS, UMR 6291, Nantes, F-44000, France. christine.sagan@chu-nantes.fr.|CHU de Nantes, Service d'anatomie et cytologique pathologiques, Nantes, France. christine.sagan@chu-nantes.fr.
authors.forename: Christine
authors.initials: C
authors.lastname: Sagan
authors.affiliation: Université de Nantes, Nantes, F-44000, France. michel.neunlist@univ-nantes.fr.|DHU2020 médecine personnalisée des maladies chroniques, Nantes, F-44000, France. michel.neunlist@univ-nantes.fr.|INSERM, UMR U913, Nantes, F-44000, France. michel.neunlist@univ-nantes.fr.
authors.forename: Michel
authors.initials: M
authors.lastname: Neunlist
authors.affiliation: INSERM, UMR U1064 and Institut de Transplantation Urologie, Néphrologie (ITUN), Nantes, F44093, France. Sophie.Brouard@univ-nantes.fr.
authors.forename: Sophie
authors.initials: S
authors.lastname: Brouard
authors.affiliation: INRA, UR1268 BIA, Nantes, F-44316, France. marie.bodinier@nantes.inra.fr.
authors.forename: Marie
authors.initials: M
authors.lastname: Bodinier
authors.affiliation: INSERM U1087, l'institut du Thorax CHU Nantes, Hôpital Laënnec, 44093, Nantes Cedex 1, France. Antoine.Magnan@univ-nantes.fr.|CNRS, UMR 6291, Nantes, F-44000, France. Antoine.Magnan@univ-nantes.fr.|Université de Nantes, Nantes, F-44000, France. Antoine.Magnan@univ-nantes.fr.|CHU de Nantes, l'institut du thorax, Service de Pneumologie, Nantes, F-44000, France. Antoine.Magnan@univ-nantes.fr.|DHU2020 médecine personnalisée des maladies chroniques, Nantes, F-44000, France. Antoine.Magnan@univ-nantes.fr.
authors.forename: Antoine
authors.initials: A
authors.lastname: Magnan
mesh_terms: D000818:Animals; D039741:Antigens, Dermatophagoides; D060829:Arthropod Proteins; D001249:Asthma / Q000276:immunology* / Q000378:metabolism / Q000503:physiopathology; D016535:Bronchial Hyperreactivity / Q000276:immunology* / Q000378:metabolism / Q000503:physiopathology; D016084:Bronchoconstriction; D018946:Chemokine CCL5 / Q000276:immunology / Q000378:metabolism; D004195:Disease Models, Animal; D005260:Female; D005512:Food Hypersensitivity / Q000276:immunology* / Q000378:metabolism; D007073:Immunoglobulin E / Q000276:immunology / Q000378:metabolism; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000276:immunology*; D008168:Lung / Q000276:immunology* / Q000378:metabolism / Q000503:physiopathology; D008807:Mice, Inbred BALB C; D010047:Ovalbumin; D010539:Permeability; D011014:Pneumonia / Q000276:immunology / Q000378:metabolism; D011657:Pulmonary Eosinophilia / Q000276:immunology / Q000378:metabolism; D018418:Th2 Cells / Q000276:immunology / Q000378:metabolism; D013997:Time Factors
chemical_list: D039741:Antigens, Dermatophagoides; D060829:Arthropod Proteins; C503460:Ccl5 protein, mouse; D018946:Chemokine CCL5; D007073:Immunoglobulin E; D010047:Ovalbumin