title: Association of IL-10 GTC haplotype with serum level and HPV infection in the development of cervical carcinoma.
abstract: OBJECTIVE
  Cervical cancer is the most common gynecological malignancy in the developing countries like India. In addition to human papillomavirus (HPV) infection, host genetic factors play an important role in viral persistence and neoplastic growth. IL-10, a multifunctional cytokine, plays an active role to promote tumor growth in the presence of HPV. The present study aims to find out the impact of IL-10 promoter polymorphisms at -1082A/G (rs1800896), -819C/T (rs1800872), and -592C/A (rs1800871) sites along with IL-10 production and HPV infection in the progression of cervical cancer.
  
  
  METHODS
  We have genotyped a total of 506 subjects, 256 cases (208 cervical cancer + 48 precancer), and 250 healthy controls by using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method followed by sequencing. IL-10 serum concentration was measured by enzyme-linked immunosorbent assay.
  
  
  RESULTS
  The frequency of IL-10 -592 variant genotype (AA) was found significantly reduced in cases as compare to controls while -1082 variant genotype (GG) was found ~4-fold higher risk of cervical cancer (p = <0.0001, OR = 3.667, 95% CI = 2.329-5.773). On construction of haplotypes, GTC haplotype was emerged as a major risk haplotype while ACA haplotype was seemed as a marker for precancerous lesions. IL-10 serum concentration was observed higher in HPV-infected precancer and cancer cases. GTC haplotype was found to be coupled with higher serum concentration of IL-10 and HPV infection.
  
  
  CONCLUSIONS
  IL-10 polymorphisms play a role in cervical cancer development and that GTC haplotype, which is closely related to its serum concentration, maybe a useful biomarker for HPV-mediated cervical cancer.
authors.affiliation: Division of Molecular Genetics and Biochemistry, Institute of Cytology and Preventive Oncology (ICMR), I-7, Sector 39, Noida, Uttar Pradesh, 201301, India.
authors.forename: Pallavi
authors.initials: P
authors.lastname: Singhal
authors.forename: Anoop
authors.initials: A
authors.lastname: Kumar
authors.forename: Soham
authors.initials: S
authors.lastname: Bharadwaj
authors.forename: Showket
authors.initials: S
authors.lastname: Hussain
authors.forename: Mausumi
authors.initials: M
authors.lastname: Bharadwaj
mesh_terms: D000328:Adult; D005260:Female; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006239:Haplotypes / Q000235:genetics; D006801:Humans; D007194:India; D016753:Interleukin-10 / Q000097:blood / Q000235:genetics*; D008875:Middle Aged; D027383:Papillomaviridae / Q000472:pathogenicity; D030361:Papillomavirus Infections / Q000097:blood / Q000235:genetics* / Q000473:pathology / Q000821:virology; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic; D002583:Uterine Cervical Neoplasms / Q000097:blood / Q000235:genetics* / Q000473:pathology / Q000821:virology
chemical_list: C508609:IL10 protein, human; D016753:Interleukin-10