title: Biocomposite of hydroxyapatite-titania rods (HApTiR): physical properties and in vitro study.
abstract: The aim in this research is to study the physical and biocompatible properties of hydroxyapatite (HAp) composites (HApTiR) having different amounts of titania rod (TiR) in them (10-90 wt.%). The HAp and TiR were produced using hydrothermal and co-precipitation under reflux methods, respectively. The physical properties and the in vitro biocompatibility of the composites to simulated body fluid (SBF) were investigated. They were also cultured with rat osteoblast-like UMR-106 cells. The synthesized powder showed a core-shell structure with the titania rod as the core and the apatite as the shell. The hardness of the composites of HApTiR's whisker increased from 74.8 to 92.9 MPa as the TiR content was increased from 10 to 90 wt.%. Mineralization study in SBF showed the formation of apatite crystals on the HApTiR's surface after 7 days of incubation. In vitro cell adhesion tests confirmed the osteoblast attachment and growth on the HApTiR's surface. The density of cells, spread and the production of calcium nodules on the substrate were seen to increase with increasing TiR contents except for HApTiR90 (TiR=90 wt.%) which exhibited lesser growth. MTT tests on HApTiR70 indicated that UMR-106 cells were viable and the density of cells on the substrate was seen to increase with increasing culturing time.
authors.affiliation: Department of Physics, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand. wponun@yahoo.com
authors.forename: Weeraphat
authors.initials: W
authors.lastname: Pon-On
authors.affiliation: Center of Calcium and Bone Research, Faculty of Science, Mahidol University, Thailand; Department of Physiology, Faculty of Science, Mahidol University, Thailand.
authors.forename: Narattaphol
authors.initials: N
authors.lastname: Charoenphandhu
authors.affiliation: ThEP Center, Commission of Higher Education, 328 Si Ayuthaya Rd., Thailand; Department of Physics, Faculty of Science, Mahidol University, Bangkok 10400, Thailand.
authors.forename: I-Ming
authors.initials: IM
authors.lastname: Tang
authors.affiliation: Center of Calcium and Bone Research, Faculty of Science, Mahidol University, Thailand; Department of Physiology, Faculty of Science, Mahidol University, Thailand.
authors.forename: Jarinthorn
authors.initials: J
authors.lastname: Teerapornpuntakit
authors.affiliation: Center of Calcium and Bone Research, Faculty of Science, Mahidol University, Thailand; Department of Physiology, Faculty of Science, Mahidol University, Thailand.
authors.forename: Jirawan
authors.initials: J
authors.lastname: Thongbunchoo
authors.affiliation: Center of Calcium and Bone Research, Faculty of Science, Mahidol University, Thailand; Department of Physiology, Faculty of Science, Mahidol University, Thailand.
authors.forename: Nateetip
authors.initials: N
authors.lastname: Krishnamra
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry* / Q000633:toxicity; D002448:Cell Adhesion / Q000187:drug effects; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D017886:Durapatite / Q000737:chemistry*; D006244:Hardness; D017348:Microscopy, Electron, Scanning Transmission; D051381:Rats; D017550:Spectroscopy, Fourier Transform Infrared; D014025:Titanium / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials; C009495:titanium dioxide; D017886:Durapatite; D014025:Titanium