title: Plasma microRNAs as potential noninvasive biomarkers for in-stent restenosis.
abstract: OBJECTIVE
  To investigate whether microRNAs (miRs) can serve as novel biomarkers for in-stent restenosis (ISR).
  
  
  METHODS
  This retrospective, observational single-centre study was conducted at the cardiovascular department of a tertiary hospital centre in the north of China. Follow-up coronary angiography at 6 to 12 months was performed in 181 consecutive patients implanted with drug-eluting stents. Fifty-two healthy volunteers served as the control group. The plasma miRs levels were analyzed by quantitative real-time PCR. Receiver-operating characteristic curve (ROC) analysis was performed to investigate the characters of these miRs as potential biomarkers of ISR.
  
  
  RESULTS
  MiR-21 levels in ISR patients were significantly higher than those in non-ISR patients and healthy controls (P<0.05), while miR-100 (P<0.05), miR-143 (P<0.001) and miR-145 (P<0.0001) levels were significantly decreased in ISR patients. Further analysis showed that miR-21 levels were remarkably increased (P = 0.045), while miR-100 (P = 0.041), miR-143 (P = 0.029) and miR-145 (P<0.01) levels were dramatically decreased in patients with diffuse ISR compared to those with focal ISR. ROC analysis demonstrated that the area under curve of miR-145, miR-143, miR-100 and miR-21 were 0.880 (95% confidence interval; CI = 0.791-0.987, P<0.001), 0.818 (95% confidence interval; CI = 0.755-0.963, P<0.001), 0.608 (95% confidence interval; CI = 0.372-0.757, P<0.05) and 0.568 (95% confidence interval; CI = 0.372-0.757, P<0.05), with specificity of 83.1%, 80.1%, 68.9% and 68.6%, and sensitivity of 88.7%, 82.1%, 60.2% and 50.1%, respectively.
  
  
  CONCLUSIONS
  Circulating miR-143 and miR-145 levels are associated with the occurrence of ISR and can serve as novel noninvasive biomarkers for ISR.
authors.affiliation: Cardiovascular Department, the First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang Province, P.R. China.
authors.forename: Meijiao
authors.initials: M
authors.lastname: He
authors.affiliation: Cardiovascular Department, the First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang Province, P.R. China.
authors.forename: Yongtai
authors.initials: Y
authors.lastname: Gong
authors.affiliation: Cardiovascular Department, the First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang Province, P.R. China.
authors.forename: Jing
authors.initials: J
authors.lastname: Shi
authors.affiliation: Department of Pharmacology, Harbin Medical University, Harbin, Heilongjiang Province, P.R. China.
authors.forename: Zhenwei
authors.initials: Z
authors.lastname: Pan
authors.affiliation: Cardiovascular Department, the Hospital of Heilongjiang Province, Harbin, Heilongjiang Province, P.R. China.
authors.forename: Hui
authors.initials: H
authors.lastname: Zou
authors.affiliation: Cardiovascular Department, the First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang Province, P.R. China.
authors.forename: Danghui
authors.initials: D
authors.lastname: Sun
authors.affiliation: Life Science and Technology College of Huazhong University of Science and Technology, Wuhan, Hubei Province, P.R. China.
authors.forename: Xin
authors.initials: X
authors.lastname: Tu
authors.affiliation: Wyeth Research, Cambridge, MA, United States of America.
authors.forename: Xiangyang
authors.initials: X
authors.lastname: Tan
authors.affiliation: Cardiovascular Department, the First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang Province, P.R. China.
authors.forename: Jianqiang
authors.initials: J
authors.lastname: Li
authors.affiliation: Cardiovascular Department, the First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang Province, P.R. China.
authors.forename: Weimin
authors.initials: W
authors.lastname: Li
authors.affiliation: Cardiovascular Department, the First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang Province, P.R. China.
authors.forename: Bin
authors.initials: B
authors.lastname: Liu
authors.affiliation: Cardiovascular Department, the First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang Province, P.R. China.
authors.forename: Jingyi
authors.initials: J
authors.lastname: Xue
authors.affiliation: Cardiovascular Department, the First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang Province, P.R. China.
authors.forename: Li
authors.initials: L
authors.lastname: Sheng
authors.affiliation: Cardiovascular Department, the First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang Province, P.R. China.
authors.forename: Chunhong
authors.initials: C
authors.lastname: Xiu
authors.affiliation: Cardiovascular Department, the First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang Province, P.R. China.
authors.forename: Ning
authors.initials: N
authors.lastname: Yang
authors.affiliation: Cardiovascular Department, the First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang Province, P.R. China.
authors.forename: Hongjie
authors.initials: H
authors.lastname: Xue
authors.affiliation: Cardiovascular Department, the First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang Province, P.R. China.
authors.forename: Xue
authors.initials: X
authors.lastname: Ding
authors.affiliation: Cardiovascular Department, the First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang Province, P.R. China.
authors.forename: Chengyuan
authors.initials: C
authors.lastname: Yu
authors.affiliation: Cardiovascular Department, the First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang Province, P.R. China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Li
mesh_terms: D000368:Aged; D015906:Angioplasty, Balloon, Coronary; D019540:Area Under Curve; D015415:Biomarkers / Q000097:blood; D016022:Case-Control Studies; D017023:Coronary Angiography; D023903:Coronary Restenosis / Q000097:blood* / Q000175:diagnosis / Q000000981:diagnostic imaging; D054855:Drug-Eluting Stents; D005260:Female; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000097:blood*; D008875:Middle Aged; D012372:ROC Curve; D012189:Retrospective Studies
chemical_list: D015415:Biomarkers; C542359:MIRN143 microRNA, human; C541010:MIRN145 microRNA, human; D035683:MicroRNAs