title: Vitamin K3 increased BMD at 1 and 2 months post-surgery and the maximum stress of the middle femur in the rat.
abstract: The therapeutic effects of vitamin K3 (VK3) on osteoporosis are still unknown. In this study, we hypothesized that VK3 possesses therapeutic effects on osteoporosis; to verify this hypothesis, the ovariectomized rat was used as an osteoporosis model. Fifty-six Sprague-Dawley female rats aged 8 to 9 months were randomly assigned to 4 groups: sham surgery, ovariectomy with saline, ovariectomy with low-dose VK3, and ovariectomy with high-dose VK3. Intramuscular injection of VK3 was performed every other day beginning 1 month postoperatively. The therapeutic effects of VK3 on osteoporosis were evaluated by measurement of bone mineral density (BMD), bone biochemical markers, biomechanical properties, and bone morphometric parameters. The overall average BMD in VK3-treated groups increased to a level between those of the ovariectomy group and the sham surgery group. The procollagen I N-terminal peptide level peaked at 2 months after surgery in all groups except in the group that had undergone ovariectomy with low-dose VK3. The tartrate-resistant acid phosphatase 5b level increased more slowly at 4 months after surgery than at 2 months after surgery in the VK3-treated groups. The ovariectomy with high-dose VK3 group had the highest maximum stress of the middle femur of all groups. With VK3 treatment, the trabecular bone area percentage increased. All morphometric indicators for the middle tibia in the VK3-treated groups reached the levels found in the sham surgery group. In summary, VK3 therapy increased BMD at 1 and 2 months postsurgery and the maximum stress of the middle femur. In addition, VK3 therapy slowed the increase in bone turnover in ovariectomized rats. Furthermore, VK3 can improve morphometric indicators for the middle tibia. Our preliminary study indicates that VK3 has a potential therapeutic effect on osteoporosis and is worthy of further investigation.
authors.affiliation: Department of Ultrasound, Tumor Hospital of Shantou University Medical College, Shantou, Guangdong, China.
authors.forename: You-jia
authors.initials: YJ
authors.lastname: Hong
authors.affiliation: Department of Nuclear Medicine, Sun Yat-sen Memorial Hospital, Sun Yat-sen University School of Medicine, Guangzhou, Guangdong, China.
authors.forename: Sheng
authors.initials: S
authors.lastname: Liu
authors.affiliation: Department of Nuclear Medicine, Sun Yat-sen Memorial Hospital, Sun Yat-sen University School of Medicine, Guangzhou, Guangdong, China.
authors.forename: Ning-yi
authors.initials: NY
authors.lastname: Jiang
authors.affiliation: Department of Radiology, Tumor Hospital of Shantou University Medical College, Shantou, Guangdong, China.
authors.forename: Sen
authors.initials: S
authors.lastname: Jiang
authors.affiliation: Department of Nuclear Medicine, Sun Yat-sen Memorial Hospital, Sun Yat-sen University School of Medicine, Guangzhou, Guangdong, China. Electronic address: liangjiugen@sohu.com.
authors.forename: Jiu-gen
authors.initials: JG
authors.lastname: Liang
mesh_terms: D000135:Acid Phosphatase / Q000378:metabolism; D000818:Animals; D015519:Bone Density / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D005260:Female; D005269:Femur / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology*; D007527:Isoenzymes / Q000378:metabolism; D010024:Osteoporosis / Q000178:diet therapy* / Q000209:etiology / Q000378:metabolism / Q000473:pathology; D010052:Ovariectomy / Q000009:adverse effects*; D010446:Peptide Fragments / Q000378:metabolism; D011347:Procollagen / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D000071681:Tartrate-Resistant Acid Phosphatase; D013997:Time Factors; D016896:Treatment Outcome; D024483:Vitamin K 3 / Q000008:administration & dosage / Q000494:pharmacology*
chemical_list: D007527:Isoenzymes; D010446:Peptide Fragments; D011347:Procollagen; D024483:Vitamin K 3; D000135:Acid Phosphatase; D000071681:Tartrate-Resistant Acid Phosphatase
keywords: Osteoporosis; Ovariectomized rats model; Procollagen I N-terminal peptide (PINP); Tartrate-resistant acid phosphatase 5b (TRACP 5b); Vitamin K(3)