title: Cytotoxic constituents of Abutilon indicum leaves against U87MG human glioblastoma cells.
abstract: The study was aimed to identify cytotoxic leads from Abutilon indicum leaves for treating glioblastoma. The petroleum ether extract, methanol extract (AIM), chloroform and ethyl acetate sub-fractions (AIM-C and AIM-E, respectively) prepared from AIM were tested for cytotoxicity on U87MG human glioblastoma cells using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. These extracts exhibited considerable activity (IC50 values of 42.6-64.5 μg/mL). The most active AIM-C fraction was repeatedly chromatographed to yield four known compounds, methyl trans-p-coumarate (1), methyl caffeate (2), syringic acid (3) and pinellic acid (4). Cell viability assay of 1-4 against U87MG cells indicated 2 as most active (IC50 value of 8.2 μg/mL), whereas the other three compounds were much less active. Interestingly, compounds 1-4 were non-toxic towards normal human cells (HEK-293). The content of 2 in AIM-C was estimated as 3% by HPLC. Hence, presence of some more active substances besides methyl caffeate (2) in AIM-C is anticipated.
authors.affiliation: a Department of Pharmacy , BITS-Pilani Hyderabad Campus, Jawahar Nagar, Shamirpet Mandal, Hyderabad 500 078 , Telangana , India.
authors.forename: Rukaiyya Sirajuddin
authors.initials: RS
authors.lastname: Khan
authors.forename: Mahibalan
authors.initials: M
authors.lastname: Senthi
authors.forename: Poorna Chandra
authors.initials: PC
authors.lastname: Rao
authors.forename: Ameer
authors.initials: A
authors.lastname: Basha
authors.forename: Mallika
authors.initials: M
authors.lastname: Alvala
authors.forename: Dinesh
authors.initials: D
authors.lastname: Tummuri
authors.forename: Hironori
authors.initials: H
authors.lastname: Masubuti
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Fujimoto
authors.forename: Ahil Sajeli
authors.initials: AS
authors.lastname: Begum
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D002109:Caffeic Acids / Q000737:chemistry; D045744:Cell Line, Tumor / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D002934:Cinnamates / Q000737:chemistry; D005231:Fatty Acids, Unsaturated / Q000737:chemistry; D005707:Gallic Acid / Q000031:analogs & derivatives / Q000737:chemistry; D005909:Glioblastoma / Q000473:pathology*; D057809:HEK293 Cells; D006801:Humans; D020128:Inhibitory Concentration 50; D019660:Malvaceae / Q000737:chemistry*; D010936:Plant Extracts / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry*
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D002109:Caffeic Acids; D002934:Cinnamates; D005231:Fatty Acids, Unsaturated; D010936:Plant Extracts; C478851:methyl-p-coumarate; C478149:pinellic acid; C042405:methyl caffeate; D005707:Gallic Acid; C001945:syringic acid
keywords: Abutilon indicum; MTT; Malvaceae; U87MG; cytotoxicity; methyl caffeate