title: Ultrasonication-assisted one-step self-assembly preparation of biocompatible fluorescent-magnetic nanobeads for rare cancer cell detection.
abstract: Multifunctional nanomaterials simultaneously possessing attractive properties, such as strong fluorescent intensity, excellent superparamagnetic behavior, easy modification and good biocompatibility, are always desired in a wide range of applications. In this work, we present a facile ultrasonication-assisted one-step self-assembly strategy for the fabrication of smart fluorescent-magnetic nanobeads (FMNBs) without using a matrix. Via one-step ultrasonication, organic-soluble superparamagnetic nanoparticles (MNPs) and quantum dots (QDs) were automatically encapsulated by amphiphilic (2-hydroxyl-3-dodecanoxyl) propylcarboxymethylchitosans (HDP-CMCHSs) through hydrophobic interaction to form hydrophilic FMNBs, presenting a good QD fluorescent property and a strong MNP magnetic response. The outer surface of the FMNBs was derived from natural biopolymer chitosans, enabling FMNBs with good biocompatibility and convenience for biological modification. As-prepared FMNBs can be easily modified with streptavidin, facilitating bioconjugation with biotin-labeled human epidermal growth factor (hEGF). hEGF-functionalized FMNBs are able to specifically recognize and capture rare target cells spiked in white blood cells, and the recovered cells can be further cultured for a long time. All of these excellent properties make nanobeads promising for circulating tumor cell (CTC) detection.
authors.affiliation: Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan University, Wuhan, 430072, People's Republic of China.
authors.forename: Shan
authors.initials: S
authors.lastname: Guo
authors.forename: Yu-Qi
authors.initials: YQ
authors.lastname: Chen
authors.forename: Ning-Ning
authors.initials: NN
authors.lastname: Lu
authors.forename: Xue-Ying
authors.initials: XY
authors.lastname: Wang
authors.forename: Min
authors.initials: M
authors.lastname: Xie
authors.forename: Wei-Ping
authors.initials: WP
authors.lastname: Sui
mesh_terms: D048271:Chitosan / Q000737:chemistry; D004815:Epidermal Growth Factor / Q000032:analysis; D006367:HeLa Cells; D006801:Humans; D061986:MCF-7 Cells; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D009360:Neoplastic Cells, Circulating / Q000737:chemistry; D045663:Quantum Dots / Q000737:chemistry*; D013010:Sonication / Q000379:methods*; D019809:Streptavidin / Q000737:chemistry
chemical_list: D058185:Magnetite Nanoparticles; D004815:Epidermal Growth Factor; D048271:Chitosan; D019809:Streptavidin