title: Pseudomonas aeruginosa induced lung injury model.
abstract: In order to study human acute lung injury and pneumonia, it is important to develop animal models to mimic various pathological features of this disease. Here we have developed a mouse lung injury model by intra-tracheal injection of bacteria Pseudomonas aeruginosa (P. aeruginosa or PA). Using this model, we were able to show lung inflammation at the early phase of injury. In addition, alveolar epithelial barrier leakiness was observed by analyzing bronchoalveolar lavage (BAL); and alveolar cell death was observed by Tunel assay using tissue prepared from injured lungs. At a later phase following injury, we observed cell proliferation required for the repair process. The injury was resolved 7 days from the initiation of P. aeruginosa injection. This model mimics the sequential course of lung inflammation, injury and repair during pneumonia. This clinically relevant animal model is suitable for studying pathology, mechanism of repair, following acute lung injury, and also can be used to test potential therapeutic agents for this disease.
authors.affiliation: Department of Pharmacology, University of Illinois College of Medicine, University of Illinois at Chicago.
authors.forename: Varsha
authors.initials: V
authors.lastname: Suresh Kumar
authors.affiliation: Section of Pulmonary and Critical Care Medicine, Atlanta VAMC, Emory University.
authors.forename: Ruxana T
authors.initials: RT
authors.lastname: Sadikot
authors.affiliation: Biologic Resources Lab, University of Illinois at Chicago.
authors.forename: Jeanette E
authors.initials: JE
authors.lastname: Purcell
authors.affiliation: Department of Pharmacology, University of Illinois College of Medicine, University of Illinois at Chicago.
authors.forename: Asrar B
authors.initials: AB
authors.lastname: Malik
authors.affiliation: Department of Pharmacology, University of Illinois College of Medicine, University of Illinois at Chicago; yuruliu@uic.edu.
authors.forename: Yuru
authors.initials: Y
authors.lastname: Liu
mesh_terms: D055371:Acute Lung Injury / Q000382:microbiology*; D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000382:microbiology; D004195:Disease Models, Animal; D051379:Mice; D011552:Pseudomonas Infections / Q000382:microbiology*; D011550:Pseudomonas aeruginosa