title: Diagnostic ability of macular nerve fiber layer thickness using new segmentation software in glaucoma suspects.
abstract: OBJECTIVE
  To assess the capacity of internal retinal layer thickness measurements made at the macula using new spectral-domain optical coherence tomography (OCT) software to distinguish between healthy subjects and those with suspected glaucoma. The diagnostic performance of such measurements also was compared with that of conventional peripapillary retinal nerve fiber layer (RNFL) thickness measurements.
  
  
  METHODS
  The study included 38 subjects with suspected glaucoma and 38 age-matched healthy subjects. In one randomly selected eye of each participant, thickness measurements at the level of the macula were made of the nerve fiber layer (mRNFL), the ganglion cell layer (GCL), and the ganglion cell complex (GCC; GCL + internal plexiform layer) through automated OCT segmentation. Peripapillary RNFL thickness (pRNFL) also was determined using the conventional scan.
  
  
  RESULTS
  As the only variable showing intergroup variation, mRNFL in the glaucoma suspects was significantly thinner in the quadrants inner inferior (P = 0.003), inner temporal (P = 0.010), and outer inferior (P = 0.017). The variable best able to discriminate between the two groups was inner inferior mRNFL thickness, as indicated by an area below the receiver operating characteristic (ROC) curve of 0.742.
  
  
  CONCLUSIONS
  Macular RNFL thickness measurements showed an improved diagnostic capacity over the other variables examined to distinguish between healthy subjects and glaucoma suspects.
authors.affiliation: Servicio de Oftalmologia, Hospital Clinico San Carlos; Departamento de Oftalmologia, Facultad de Medicina, Universidad Complutense de Madrid; and Instituto de Investigacion Sanitaria del Hospital Clinico San Carlos (IdISSC), Madrid, Spain.
authors.forename: Jose M
authors.initials: JM
authors.lastname: Martinez-de-la-Casa
authors.affiliation: Servicio de Oftalmologia, Hospital Clinico San Carlos; Departamento de Oftalmologia, Facultad de Medicina, Universidad Complutense de Madrid; and Instituto de Investigacion Sanitaria del Hospital Clinico San Carlos (IdISSC), Madrid, Spain.
authors.forename: Pilar
authors.initials: P
authors.lastname: Cifuentes-Canorea
authors.affiliation: Servicio de Oftalmologia, Hospital Clinico San Carlos; Departamento de Oftalmologia, Facultad de Medicina, Universidad Complutense de Madrid; and Instituto de Investigacion Sanitaria del Hospital Clinico San Carlos (IdISSC), Madrid, Spain.
authors.forename: Clara
authors.initials: C
authors.lastname: Berrozpe
authors.affiliation: Servicio de Oftalmologia, Hospital Clinico San Carlos; Departamento de Oftalmologia, Facultad de Medicina, Universidad Complutense de Madrid; and Instituto de Investigacion Sanitaria del Hospital Clinico San Carlos (IdISSC), Madrid, Spain.
authors.forename: Marina
authors.initials: M
authors.lastname: Sastre
authors.affiliation: Servicio de Oftalmología, Hospital Miguel Servet, Zaragoza, Spain.
authors.forename: Vicente
authors.initials: V
authors.lastname: Polo
authors.affiliation: Servicio de Oftalmología, Clinica Universitaria de Navarra, Pamplona, Spain.
authors.forename: Javier
authors.initials: J
authors.lastname: Moreno-Montañes
authors.affiliation: Servicio de Oftalmologia, Hospital Clinico San Carlos; Departamento de Oftalmologia, Facultad de Medicina, Universidad Complutense de Madrid; and Instituto de Investigacion Sanitaria del Hospital Clinico San Carlos (IdISSC), Madrid, Spain.
authors.forename: Julian
authors.initials: J
authors.lastname: Garcia-Feijoo
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D003430:Cross-Sectional Studies; D003936:Diagnosis, Computer-Assisted / Q000379:methods*; D003937:Diagnosis, Differential; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D007429:Intraocular Pressure; D008297:Male; D008875:Middle Aged; D009412:Nerve Fibers / Q000473:pathology*; D009798:Ocular Hypertension / Q000175:diagnosis* / Q000503:physiopathology; D012372:ROC Curve; D015203:Reproducibility of Results; D012165:Retinal Ganglion Cells / Q000473:pathology*; D012189:Retrospective Studies; D012984:Software; D041623:Tomography, Optical Coherence / Q000379:methods*
keywords: ganglion cell; glaucoma; macula; optical coherence tomography; retinal nerve fiber layer