title: Impact of dietary protein level and source of polyunsaturated fatty acids on lipid metabolism-related protein expression and fatty acid concentrations in porcine tissues.
abstract: The study assessed the effects of reduced protein (RPD) vs high protein diet (HPD) in combination with n-3/n-6 PUFA-containing plant oils [linseed oil (LO)/sunflower seed oil (SO)] supplementation on lipid metabolism-related protein expression and fatty acid concentrations in porcine tissues. Forty male Landrace pigs (castrates) were allocated into four groups fed diets different in dietary protein and PUFA level and one control group. SCD-1 protein expression in pig muscle, back fat, and liver was not affected by diet. The protein expression of precursor (pSREBP-1c) and active nuclear form of SREBP-1c (mSREBP-1c) in muscle and back fat was affected by diet, however not in liver of pigs. In contrast, the expression of ACC and FAS expression was significantly affected by diet only in the liver. The fatty acid concentrations in muscle, liver, and back fat resulted in higher n-3 PUFA concentrations of LO groups compared to the SO groups.
authors.affiliation: Institute of Muscle Biology and Growth and Institute of Genetics and Biometry, Leibniz Institute for Farm Animal Biology , Wilhelm-Stahl-Allee 2, 18196 Dummerstorf, Germany.
authors.forename: Dirk
authors.initials: D
authors.lastname: Dannenberger
authors.forename: Karin
authors.initials: K
authors.lastname: Nuernberg
authors.forename: Gerd
authors.initials: G
authors.lastname: Nuernberg
authors.forename: Antje
authors.initials: A
authors.lastname: Priepke
mesh_terms: D000103:Acetyl-CoA Carboxylase / Q000235:genetics / Q000378:metabolism; D000818:Animals; D004044:Dietary Proteins / Q000378:metabolism*; D005231:Fatty Acids, Unsaturated / Q000378:metabolism*; D050356:Lipid Metabolism; D008297:Male; D008460:Meat / Q000032:analysis; D018482:Muscle, Skeletal / Q000201:enzymology / Q000378:metabolism*; D010938:Plant Oils / Q000378:metabolism*; D013230:Stearoyl-CoA Desaturase / Q000235:genetics / Q000378:metabolism; D051780:Sterol Regulatory Element Binding Protein 1 / Q000235:genetics / Q000378:metabolism; D013552:Swine / Q000235:genetics* / Q000378:metabolism*
chemical_list: D004044:Dietary Proteins; D005231:Fatty Acids, Unsaturated; D010938:Plant Oils; D051780:Sterol Regulatory Element Binding Protein 1; D013230:Stearoyl-CoA Desaturase; D000103:Acetyl-CoA Carboxylase
keywords: PUFA; adipose tissue; lipid metabolism; muscle; pig; plant oils; reduced protein diet