title: Expression and clinical significance of miRNA-34a in colorectal cancer.
abstract: BACKGROUND
  The aim of this study was to investigate differences of miRNA-34a expression in benign and malignant colorectal lesions.
  
  
  METHODS
  Samples of cancer, paraneoplastic tissues and polyps were selected and total RNA was extracted by conventional methods for real-time PCR to detect the miRNA- 34a expression. In addition, the LOVO colorectal cancer cell line was cultured, treated with the demethylating agent 5-azacytidine and screened for differentially expressed miRNA-34a.
  
  
  RESULTS
  After the drug treatment, the miRNA-34a expression of colorectal cancer cell line LOVO was increased and real-time PCR showed that levels of expression in both cell line and colorectal cancer tissues were low, as compared to paraneoplastic tissue (p<0.05). Polyps tissues had significantly higher expression than paraneoplastic and colorectal cancer samples (p<0.05).
  
  
  CONCLUSIONS
  miRNA-34a-5p may play a role as a tumor suppressor gene in colorectal cancer, with involvement of DNA methylation.
authors.affiliation: Department of Gastroenterology, the First Affiliated Hospital of Harbin Medical University, Harbin, China E-mail : yinghualicn@163.com.
authors.forename: Zhi-Bin
authors.initials: ZB
authors.lastname: Ma
authors.forename: Xiao-Lin
authors.initials: XL
authors.lastname: Kong
authors.forename: Gang
authors.initials: G
authors.lastname: Cui
authors.forename: Cui-Cui
authors.initials: CC
authors.lastname: Ren
authors.forename: Ying-Jie
authors.initials: YJ
authors.lastname: Zhang
authors.forename: Sheng-Jin
authors.initials: SJ
authors.lastname: Fan
authors.forename: Ying-Hua
authors.initials: YH
authors.lastname: Li
mesh_terms: D000230:Adenocarcinoma / Q000737:chemistry / Q000235:genetics*; D000236:Adenoma / Q000737:chemistry / Q000235:genetics*; D000368:Aged; D045744:Cell Line, Tumor; D003106:Colon / Q000737:chemistry; D003111:Colonic Polyps / Q000737:chemistry / Q000235:genetics*; D015179:Colorectal Neoplasms / Q000737:chemistry / Q000235:genetics* / Q000473:pathology; D019175:DNA Methylation; D005260:Female; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000032:analysis / Q000235:genetics*; D008875:Middle Aged; D012333:RNA, Messenger / Q000032:analysis; D012007:Rectum / Q000737:chemistry
chemical_list: C522425:MIRN34 microRNA, human; D035683:MicroRNAs; D012333:RNA, Messenger