title: Effect of temperature on denitrifying methanotrophic activity of 'Candidatus Methylomirabilis oxyfera'.
abstract: The activity of denitrifying methanotrophic bacteria at 11-30 °C was assessed in short-term experiments. The aim was to determine the feasibility of applying denitrifying methanotrophic bacteria in low-temperature anaerobic wastewater treatment. This study showed that biomass enriched at 21 °C had an optimum temperature of 20-25 °C and that activity dropped as temperature was increased to 30 °C. Biomass enriched at 30 °C had an optimum temperature of 25-30 °C. These results indicated that biomass from low-temperature inocula adjusted to the enrichment temperature and that low-temperature enrichment is suitable for applications in low-temperature wastewater treatment. Biomass growth at ≤20 °C still needs to be studied.
authors.affiliation: Present address: Department of Chemical and Biological Engineering, Industrial Biotechnology, Chalmers University of Technology, Kemivägen 10, 412 96 Gothenburg, Sweden E-mail: christel.kampman@chalmers.se.
authors.forename: Christel
authors.initials: C
authors.lastname: Kampman
authors.affiliation: Present address: Rodovia Akzo Nobel 707, 13295-000, Itupeva - São Paulo, Brazil.
authors.forename: Laura
authors.initials: L
authors.lastname: Piai
authors.affiliation: Sub-Department of Environmental Technology, Wageningen University, P.O. Box 17, 6700 AA Wageningen, The Netherlands.
authors.forename: Tim L G
authors.initials: TL
authors.lastname: Hendrickx
authors.affiliation: Sub-Department of Environmental Technology, Wageningen University, P.O. Box 17, 6700 AA Wageningen, The Netherlands.
authors.forename: Hardy
authors.initials: H
authors.lastname: Temmink
authors.affiliation: Sub-Department of Environmental Technology, Wageningen University, P.O. Box 17, 6700 AA Wageningen, The Netherlands.
authors.forename: Grietje
authors.initials: G
authors.lastname: Zeeman
authors.affiliation: Sub-Department of Environmental Technology, Wageningen University, P.O. Box 17, 6700 AA Wageningen, The Netherlands.
authors.forename: Cees J N
authors.initials: CJ
authors.lastname: Buisman
mesh_terms: D001419:Bacteria / Q000378:metabolism*; D018533:Biomass; D003080:Cold Temperature; D058440:Denitrification; D014865:Waste Disposal, Fluid