title: Characterization of the enterovirus 71 P1 polyprotein expressed in Pichia pastor as a candidate vaccine.
abstract: Human enterovirus 71 (EV71) plays an important role in hand, foot, and mouth disease (HFMD), which recently caused the death of hundreds of children in the Asia-Pacific region. However, there are no specific treatments available for EV71 infections; thus, a safe and effective vaccine is needed urgently. In this study, we developed an effective and economical method for producing EV71 polyprotein (P1 protein) in Pichia pastoris. Furthermore, we evaluated the potential of P1 protein as a candidate vaccine against EV71 virus. The data revealed that P1 protein induced persistent high cross-neutralization antibodies for different EV71 subtypes, and elicited significant splenocyte proliferation. The high levels of interleukin-10(IL-10) and interferon-gamma (IFN-γ) showed that P1 protein induced Th1 and Th2 immune responses. Interestingly, vaccinating female mice with the P1 protein conferred cross-protection against different EV71 subtypes to their neonatal offspring.Compared with heat-inactivated EV71, the P1 protein elicited improved humoral and cellular immune responses and showed good cross-protection with different EV71 subtypes. Therefore, the EV71-P1 protein produced by P. pastoris is a promising candidate vaccine against EV71.
authors.affiliation: a College of life science; Jianghan University; Wuhan, Hubei, PR China.
authors.forename: Xue
authors.initials: X
authors.lastname: Han
authors.forename: Xiao-ling
authors.initials: XL
authors.lastname: Ying
authors.forename: Shi-li
authors.initials: SL
authors.lastname: Zhou
authors.forename: Tao
authors.initials: T
authors.lastname: Han
authors.forename: Hao
authors.initials: H
authors.lastname: Huang
authors.forename: Qi
authors.initials: Q
authors.lastname: Jin
authors.forename: Fan
authors.initials: F
authors.lastname: Yang
authors.forename: Qi-ying
authors.initials: QY
authors.lastname: Sun
authors.forename: Xian-xun
authors.initials: XX
authors.lastname: Sun
mesh_terms: D000818:Animals; D057134:Antibodies, Neutralizing / Q000097:blood; D000914:Antibodies, Viral / Q000097:blood; D049109:Cell Proliferation; D003429:Cross Reactions; D029821:Enterovirus A, Human / Q000235:genetics / Q000276:immunology*; D005260:Female; D015870:Gene Expression; D006232:Hand, Foot and Mouth Disease / Q000517:prevention & control*; D007371:Interferon-gamma / Q000378:metabolism; D007963:Leukocytes, Mononuclear / Q000276:immunology; D008807:Mice, Inbred BALB C; D010843:Pichia / Q000235:genetics / Q000254:growth & development; D020815:Polyproteins / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification; D014765:Viral Vaccines / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification
chemical_list: D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D020815:Polyproteins; D011994:Recombinant Proteins; D014614:Vaccines, Synthetic; D014765:Viral Vaccines; D007371:Interferon-gamma
keywords: P1 polyprotein; Pichia pastoris; enterovirus 71; immunogenicity vaccine