title: Secretome analysis revealed adaptive and non-adaptive responses of the Staphylococcus carnosus femB mutant.
abstract: FemABX peptidyl transferases are involved in non-ribosomal pentaglycine interpeptide bridge biosynthesis. Here, we characterized the phenotype of a Staphylococcus carnosus femB deletion mutant, which was affected in growth and showed pleiotropic effects such as enhanced methicillin sensitivity, lysostaphin resistance, cell clustering, and decreased peptidoglycan cross-linking. However, comparative secretome analysis revealed a most striking difference in the massive secretion or release of proteins into the culture supernatant in the femB mutant than the wild type. The secreted proteins can be categorized into typical cytosolic proteins and various murein hydrolases. As the transcription of the murein hydrolase genes was up-regulated in the mutant, they most likely represent an adaption response to the life threatening mutation. Even though the transcription of the cytosolic protein genes was unaltered, their high abundance in the supernatant of the mutant is most likely due to membrane leakage triggered by the weakened murein sacculus and enhanced autolysins.
authors.affiliation: Microbial Genetics, Interfaculty Institute of Microbiology and Infection Medicine, University of Tübingen, Tübingen, Germany.
authors.forename: Mulugeta
authors.initials: M
authors.lastname: Nega
authors.forename: Linda
authors.initials: L
authors.lastname: Dube
authors.forename: Melanie
authors.initials: M
authors.lastname: Kull
authors.forename: Anne-Kathrin
authors.initials: AK
authors.lastname: Ziebandt
authors.forename: Patrick
authors.initials: P
authors.lastname: Ebner
authors.forename: Dirk
authors.initials: D
authors.lastname: Albrecht
authors.forename: Bernhard
authors.initials: B
authors.lastname: Krismer
authors.forename: Ralf
authors.initials: R
authors.lastname: Rosenstein
authors.forename: Michael
authors.initials: M
authors.lastname: Hecker
authors.forename: Friedrich
authors.initials: F
authors.lastname: Götz
mesh_terms: D000222:Adaptation, Physiological; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D017384:Sequence Deletion; D013210:Staphylococcus / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic; D015854:Up-Regulation
chemical_list: D001426:Bacterial Proteins
keywords: Cytosolic proteins; FemB; Microbiology; Peptidoglycan; Secretome; Staphylococcus carnosus