title: A label-free and sensitive fluorescent method for the detection of uracil-DNA glycosylase activity.
abstract: The activity of uracil-DNA glycosylase (UDG), an enzyme in the base excision repair, is detected at a high sensitivity by a DNA substrate containing only one uracil through a label-free fluorescent approach, which is also successfully applied for the measurement of UDG inhibitors.
authors.affiliation: Department of Chemistry, Beijing Key Laboratory for Microanalytical Methods and Instrumentation, Key Laboratory of Bioorganic Phosphorus Chemistry and Chemical Biology (Ministry of Education), Tsinghua University, Beijing 100084, China. xiang-yu@tsinghua.edu.cn.
authors.forename: Jing
authors.initials: J
authors.lastname: Tao
authors.forename: Panshu
authors.initials: P
authors.lastname: Song
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Sato
authors.forename: Seiichi
authors.initials: S
authors.lastname: Nishizawa
authors.forename: Norio
authors.initials: N
authors.lastname: Teramae
authors.forename: Aijun
authors.initials: A
authors.lastname: Tong
authors.forename: Yu
authors.initials: Y
authors.lastname: Xiang
mesh_terms: D001483:Base Sequence; D004247:DNA / Q000235:genetics / Q000378:metabolism; D057075:Enzyme Assays / Q000379:methods*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D013050:Spectrometry, Fluorescence; D014498:Uracil / Q000378:metabolism; D051981:Uracil-DNA Glycosidase / Q000037:antagonists & inhibitors / Q000378:metabolism*
chemical_list: D004791:Enzyme Inhibitors; D014498:Uracil; D004247:DNA; D051981:Uracil-DNA Glycosidase