title: Improving the cytological diagnosis of intraocular lymphoma from vitreous fluid.
abstract: OBJECTIVE
  To improve the cytological diagnosis of retinal lymphoma on vitreous fluid using improved cell collection and systematic analyses.
  
  
  RESULTS
  Since October 2010, we have developed and optimized in our department a method with which to perform the diagnosis of retinal lymphoma. The vitreous sample was collected in a tube containing RPMI-1640 medium, decomplemented fetal bovine serum, and gentamicin. The transport and technical steps were performed at 4°C. Systematically, cytological examination with May-Grünwald-Giemsa staining and immunocytochemistry (mainly anti-CD3, anti-CD20 and anti-CD68 antibodies) were performed on cytospins. Whenever possible, determination of B-cell clonality, flow cytometry and determination of the interleukin (IL)-10/IL-6 ratio were performed. From October 2010 to June 2013, with this optimized protocol, 38 vitreous cytological samples from 32 patients were analysed, and a final diagnosis was possible, avoiding a biopsy, in all cases except one.
  
  
  CONCLUSIONS
  The preservation of vitreous fluid cells on culture medium led to the diagnosis of retinal lymphoma in 10 of 12 cases, and exclusion of this diagnosis in 26 cases. This protocol may be applied even when the delay in shipping from the surgery to the pathology departments exceeds 1 h.
authors.affiliation: Pathology and Histology-Cytology Department, Toulouse-Rangueil University Hospital, Toulouse, France.
authors.forename: Marie-Laure
authors.initials: ML
authors.lastname: Ranty
authors.affiliation: Pathology Department, Toulouse-Purpan University Hospital, Toulouse, France.
authors.forename: Camille
authors.initials: C
authors.lastname: Laurent
authors.affiliation: Pathology and Histology-Cytology Department, Toulouse-Rangueil University Hospital, Toulouse, France.
authors.forename: Jacqueline
authors.initials: J
authors.lastname: Aziza
authors.affiliation: Ophthalmology Department, Toulouse-Purpan University Hospital, Toulouse, France.
authors.forename: Laurence
authors.initials: L
authors.lastname: Mahieu
authors.affiliation: Ophthalmology Department, Toulouse-Purpan University Hospital, Toulouse, France.
authors.forename: Priscille
authors.initials: P
authors.lastname: Olle
authors.affiliation: Ophthalmology Department, Toulouse-Purpan University Hospital, Toulouse, France.
authors.forename: Jean-Claude
authors.initials: JC
authors.lastname: Quintyn
authors.affiliation: Immunology Department, Lyon-South University Hospital, Pierre Bénite, France.
authors.forename: Lorna
authors.initials: L
authors.lastname: Garnier
authors.affiliation: Immunology Department, Lyon-South University Hospital, Pierre Bénite, France.
authors.forename: Jacques
authors.initials: J
authors.lastname: Bienvenu
authors.affiliation: Pathology and Histology-Cytology Department, Toulouse-Rangueil University Hospital, Toulouse, France.
authors.forename: Laetitia
authors.initials: L
authors.lastname: Collin
authors.affiliation: Pathology and Histology-Cytology Department, Toulouse-Rangueil University Hospital, Toulouse, France.
authors.forename: Marie-Bernadette
authors.initials: MB
authors.lastname: Delisle
authors.affiliation: Pathology and Histology-Cytology Department, Toulouse-Rangueil University Hospital, Toulouse, France.
authors.forename: Monique
authors.initials: M
authors.lastname: Courtade-Saidi
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001402:B-Lymphocytes / Q000473:pathology; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D016753:Interleukin-10 / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D008228:Lymphoma, Non-Hodgkin / Q000175:diagnosis* / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D061251:Primary Cell Culture; D019572:Retinal Neoplasms / Q000175:diagnosis* / Q000378:metabolism; D012189:Retrospective Studies; D014821:Vitrectomy; D014822:Vitreous Body / Q000473:pathology*
chemical_list: D015850:Interleukin-6; D016753:Interleukin-10
keywords: clonality; cytology; immunocytochemistry; interleukin; retinal lymphoma