title: [Modulating expression of key genes within β-carotene synthetic pathway in recombinant Escherichia coli with RBS library to improve β-carotene production].
abstract: β-carotene belongs to carotenoids family, widely applied in pharmaceuticals, neutraceuticals, cosmetics and food industries. In this study, three key genes (dxs, idi, and crt operon) within β-carotene synthetic pathway in recombinant Escherichia coli strain CAR005 were modulated with RBS Library to improve β-carotene production. There were 7%, 11% and 17% increase of β-carotene yield respectively after modulating dxs, idi and crt operon genes with RBS Library, demonstrating that modulating gene expression with regulatory parts libraries would have more opportunities to obtain optimal production of target compound. Combined modulation of crt operon, dxs and idi genes led to 35% increase of β-carotene yield compared to parent strain CAR005. The optimal gene expression strength identified in single gene modulation would not be the optimal strength when used in combined modulation. Our study provides a new strategy for improving production of target compound through modulation of gene expression.
authors.forename: Guanping
authors.initials: G
authors.lastname: Dai
authors.forename: Tao
authors.initials: T
authors.lastname: Sun
authors.forename: Liangtian
authors.initials: L
authors.lastname: Miao
authors.forename: Qingyan
authors.initials: Q
authors.lastname: Li
authors.forename: Dongguang
authors.initials: D
authors.lastname: Xiao
authors.forename: Xueli
authors.initials: X
authors.lastname: Zhang
mesh_terms: D004926:Escherichia coli / Q000378:metabolism*; D015870:Gene Expression; D015723:Gene Library; D009876:Operon; D019207:beta Carotene / Q000096:biosynthesis*
chemical_list: D019207:beta Carotene