title: [Compound Huang Gan delays chronic renal failure after 5/6 nephrectomy in rats].
abstract: OBJECTIVE
  To observe the effect of compound Huang Gan in delaying chronic renal failure in rats after 5/6 nephrectomy and explore the possible mechanisms.
  
  
  METHODS
  High-performance liquid chromatography was used to was used identify the components of compound Huang Gan extract. Rat models of 5/6 nephrectomy received a 12-week treatment with intragastric administration of Niaoduqing, Cozaar, or compound Huang Gan at low, moderate or high doses (n=10). After the treatments, the rats were sacrificed for detecting Scr, BUN, Ucr and 24h UPr , pathological examination of the renal tissues, and determination of FN, MCP-1, and ICAM-1 expression levels in the renal tissues using RT-PCR and immunohistochemistry.
  
  
  RESULTS
  The major chemical components of compound Huang Gan extract included glycyrrhizin (0.61%), paeonol (1.2%), aloe emodin (0.72%), rhein (0.85%), emodin (0.87%), chrysophanol (0.79%) and physcion (0.8%). Treatment with compound Huang Gan at low, moderate and high doses significantly reduced Scr, BUN, Ucr , Ccr and 24 h UPr levels (P(P<0.05), improved interstitial fibrosis and glomerulosclerosis, and reduced FN and ICAM-1 expressions (P(P<0.05) in rats following nephrectomy.
  
  
  CONCLUSIONS
  Compound Huang Gan can improve the renal function and lessen glomerulosclerosis and renal interstitial fibrosis to delay the progression of chronic renal failure in rat models of 5/6 nephrectomy.
authors.affiliation: Department of Pharmacy, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. E-mail: xxyzjh119@163.com.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Xiao
authors.forename: Liqian
authors.initials: L
authors.lastname: Mo
authors.forename: Shaolian
authors.initials: S
authors.lastname: Song
authors.forename: Min
authors.initials: M
authors.lastname: Qin
authors.forename: Xixiao
authors.initials: X
authors.lastname: Yang
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005353:Fibronectins / Q000378:metabolism; D005355:Fibrosis; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism; D007668:Kidney / Q000473:pathology; D007676:Kidney Failure, Chronic / Q000188:drug therapy*; D009392:Nephrectomy; D051381:Rats
chemical_list: D004365:Drugs, Chinese Herbal; D005353:Fibronectins; D018799:Intercellular Adhesion Molecule-1