title: Transposition-mediated DNA re-replication in maize.
abstract: Every DNA segment in a eukaryotic genome normally replicates once and only once per cell cycle to maintain genome stability. We show here that this restriction can be bypassed through alternative transposition, a transposition reaction that utilizes the termini of two separate, nearby transposable elements (TEs). Our results suggest that alternative transposition during S phase can induce re-replication of the TEs and their flanking sequences. The DNA re-replication can spontaneously abort to generate double-strand breaks, which can be repaired to generate Composite Insertions composed of transposon termini flanking segmental duplications of various lengths. These results show how alternative transposition coupled with DNA replication and repair can significantly alter genome structure and may have contributed to rapid genome evolution in maize and possibly other eukaryotes.
authors.affiliation: Department of Genetics, Development and Cell Biology, Iowa State University, Ames, United States.
authors.forename: Jianbo
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Genetics, Development and Cell Biology, Iowa State University, Ames, United States.
authors.forename: Tao
authors.initials: T
authors.lastname: Zuo
authors.affiliation: Department of Genetics, Development and Cell Biology, Iowa State University, Ames, United States.
authors.forename: Dafang
authors.initials: D
authors.lastname: Wang
authors.affiliation: Department of Genetics, Development and Cell Biology, Iowa State University, Ames, United States.
authors.forename: Thomas
authors.initials: T
authors.lastname: Peterson
mesh_terms: D000483:Alleles; D020029:Base Pairing / Q000235:genetics; D059766:DNA End-Joining Repair / Q000235:genetics; D004261:DNA Replication / Q000235:genetics*; D004251:DNA Transposable Elements / Q000235:genetics*; D018744:DNA, Plant / Q000235:genetics; D020440:Gene Duplication; D056426:Genetic Loci; D018745:Genome, Plant; D008957:Models, Genetic; D016254:Mutagenesis, Insertional / Q000235:genetics; D016133:Polymerase Chain Reaction; D011995:Recombination, Genetic; D003313:Zea mays / Q000235:genetics*
chemical_list: D004251:DNA Transposable Elements; D018744:DNA, Plant
keywords: Ac/Ds; DNA replication; chromosomes; composite insertion; duplication; genes; maize; plant biology; transposition