title: Determination of catecholamines in urine using aminophenylboronic acid functionalized magnetic nanoparticles extraction followed by high-performance liquid chromatography and electrochemical detection.
abstract: A new method was developed for the simultaneous determination of three catecholamines in urine using aminophenylboronic acid functionalized magnetic nanoparticles extraction followed by high-performance liquid chromatography with electrochemical detection. Novel aminophenylboronic acid functionalized magnetic nanoparticles were prepared by multi-step covalent modification, and characterized by transmission electron microscopy, Fourier-transformed infrared spectroscopy, X-ray diffraction, and vibrating sample magnetometry. With the help of the high affinity between the boronate and cis-diol group, the particles were used for the highly selective separation and enrichment of three major catecholamines, norepinephrine, epinephrine, and dopamine. Effects of the pH of the feed solution, the extraction time, the composition of the buffer solution, the amount of the magnetic particles, the elution conditions, and the recycling of aminophenylboronic acid functionalized magnetic nanoparticles were explored. Under the optimized conditions, 13-17-fold enrichment factors were obtained. The linear ranges were 0.01-2.0 μg/mL for the studied analytes. The limits of detection and quantification were in the range of 2.0-7.9 and 6.7-26.3 ng/mL, respectively. The relative recoveries were in the range of 92-108%, with intraday and interday relative standard deviations lower than 6.8%. This method was successfully applied to analysis of catecholamines in real urine.
authors.affiliation: Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research (Ministry of Education of China), Key Laboratory of Phytochemical R&D of Hunan Province, Hunan Normal University, Changsha, PR China.
authors.forename: Liwei
authors.initials: L
authors.lastname: Jiang
authors.forename: Yibang
authors.initials: Y
authors.lastname: Chen
authors.forename: Yanmei
authors.initials: Y
authors.lastname: Luo
authors.forename: Yueming
authors.initials: Y
authors.lastname: Tan
authors.forename: Ming
authors.initials: M
authors.lastname: Ma
authors.forename: Bo
authors.initials: B
authors.lastname: Chen
authors.forename: Qingji
authors.initials: Q
authors.lastname: Xie
authors.forename: Xubiao
authors.initials: X
authors.lastname: Luo
mesh_terms: D000328:Adult; D001897:Boronic Acids / Q000737:chemistry*; D002395:Catecholamines / Q000652:urine*; D002851:Chromatography, High Pressure Liquid; D055664:Electrochemical Techniques; D005260:Female; D064368:Healthy Volunteers; D006801:Humans; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D008297:Male; D055815:Young Adult
chemical_list: D001897:Boronic Acids; D002395:Catecholamines; D058185:Magnetite Nanoparticles; C028592:3-aminobenzeneboronic acid
keywords: Boronate affinity interaction; Catecholamines; Electrochemical detection; Magnetic nanoparticles; Sample preparation