title: Interactions between gold nanoparticles and amyloid β25-35 peptide.
abstract: Amyloid β25-35 (Aβ25-35) peptide is a peculiar peptide for its rapid aggregation properties and high neurotoxicity in Alzheimer's disease. Here, the interactions between gold nanoparticles (GNPs) and Aβ25-35 monomers, oligomers and fibrils are explored under different molar ratio, temperature and pH by ultraviolet-visible and circular dichroism spectra, thioflavin T fluorescence assay and transmission electron microscope. It is concluded that Aβ25-35 can induce the aggregation of GNPs at certain concentration of Aβ25-35 monomer or oligomer. But at higher concentration of Aβ25-35, GNPs aggregates dissociate again. Furthermore, the aggregation rate increases at higher temperature or for lower pH. These results might provide the basis of a simple diagnostic tool for detecting Alzheimer's disease.
authors.affiliation: Department of Biomaterials, College of Materials, Xiamen University, Xiamen 361005, People's Republic of China.
authors.forename: Jian
authors.initials: J
authors.lastname: Peng
authors.affiliation: Department of Biomaterials, College of Materials, Xiamen University, Xiamen 361005, People's Republic of China.
authors.forename: Jian
authors.initials: J
authors.lastname: Weng
authors.affiliation: Department of Biomaterials, College of Materials, Xiamen University, Xiamen 361005, People's Republic of China.
authors.forename: Lei
authors.initials: L
authors.lastname: Ren
authors.affiliation: Department of Biomaterials, College of Materials, Xiamen University, Xiamen 361005, People's Republic of China. sunliping@xmu.edu.cn.
authors.forename: Li-Ping
authors.initials: LP
authors.lastname: Sun
mesh_terms: D000682:Amyloid / Q000737:chemistry / Q000378:metabolism; D016229:Amyloid beta-Peptides / Q000737:chemistry* / Q000378:metabolism*; D006046:Gold / Q000737:chemistry* / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D053768:Metal Nanoparticles / Q000737:chemistry*; D010446:Peptide Fragments / Q000737:chemistry* / Q000378:metabolism*; D013696:Temperature
chemical_list: D000682:Amyloid; D016229:Amyloid beta-Peptides; D010446:Peptide Fragments; C074636:amyloid beta-protein (25-35); D006046:Gold