title: Beneficial effect of tolerogenic dendritic cells pulsed with MOG autoantigen in experimental autoimmune encephalomyelitis.
abstract: BACKGROUND
  Treatment with tolerogenic dendritic cells (TolDC) is a promising, cell-based strategy to regulate autoimmune diseases such as multiple sclerosis (MS) in an antigen-specific way. This technique involves the use of TolDC from MS patients cultured in the presence of vitamin D(3) (VitD3) and pulsed with myelin peptides to induce a stable hyporesponsiveness in myelin-specific autologous T cells.
  
  
  OBJECTIVE
  The purpose of this study was to analyze the in vivo effect of VitD3-TolDC treatment on experimental autoimmune encephalomyelitis, an animal model of MS.
  
  
  METHODS
  Bone marrow-derived TolDC cultured in the presence of VitD3 and pulsed with peptide 40-55 of the myelin oligodendrocyte glycoprotein (MOG(40-55)) were administrated preventively, preclinically, and therapeutically to EAE-induced mice.
  
  
  RESULTS
  We found that VitD3-TolDC-MOG treatment showed a beneficial effect, not only decreasing the incidence of the disease but also reducing the severity of the clinical signs mediated by induction of regulatory T cells (Treg), as well as IL-10 production and reduction of Ag-specific lymphoproliferation. Our results support VitD3-TolDC-peptide(s) treatment as a potential strategy to restore tolerance in autoimmune diseases such as MS.
authors.affiliation: Division of Immunology, Germans Trias i Pujol University Hospital and Research Institute, Campus Can Ruti, Badalona, Spain; Department of Cellular Biology, Physiology and Immunology, Universitat Autònoma de Barcelona, Cerdanyola del Vallès, Spain.
authors.forename: María José
authors.initials: MJ
authors.lastname: Mansilla
authors.forename: Carla
authors.initials: C
authors.lastname: Sellès-Moreno
authors.forename: Sara
authors.initials: S
authors.lastname: Fàbregas-Puig
authors.forename: Joan
authors.initials: J
authors.lastname: Amoedo
authors.forename: Juan
authors.initials: J
authors.lastname: Navarro-Barriuso
authors.forename: Aina
authors.initials: A
authors.lastname: Teniente-Serra
authors.forename: Laia
authors.initials: L
authors.lastname: Grau-López
authors.forename: Cristina
authors.initials: C
authors.lastname: Ramo-Tello
authors.forename: Eva M
authors.initials: EM
authors.lastname: Martínez-Cáceres
mesh_terms: D000818:Animals; D001324:Autoantigens / Q000378:metabolism*; D001854:Bone Marrow Cells / Q000502:physiology; D018929:Cell Culture Techniques; D002465:Cell Movement / Q000502:physiology; D017690:Cell Transplantation / Q000379:methods*; D002478:Cells, Cultured; D002762:Cholecalciferol / Q000378:metabolism; D003713:Dendritic Cells / Q000473:pathology / Q000502:physiology / Q000637:transplantation*; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000473:pathology / Q000503:physiopathology / Q000628:therapy*; D005260:Female; D016753:Interleukin-10 / Q000378:metabolism; D008810:Mice, Inbred C57BL; D063308:Myelin-Oligodendrocyte Glycoprotein / Q000276:immunology*; D012720:Severity of Illness Index; D013154:Spleen / Q000473:pathology / Q000503:physiopathology; D050378:T-Lymphocytes, Regulatory / Q000502:physiology
chemical_list: D001324:Autoantigens; C556062:IL10 protein, mouse; C571327:Mog protein, mouse; D063308:Myelin-Oligodendrocyte Glycoprotein; D016753:Interleukin-10; D002762:Cholecalciferol
keywords: Dendritic cells; Experimental autoimmune encephalomyelitis; Regulatory T cells; Tolerance; Vitamin D3