title: Current peptidomics: applications, purification, identification, quantification, and functional analysis.
abstract: Peptidomics is an emerging field branching from proteomics that targets endogenously produced protein fragments. Endogenous peptides are often functional within the body-and can be both beneficial and detrimental. This review covers the use of peptidomics in understanding digestion, and identifying functional peptides and biomarkers. Various techniques for peptide and glycopeptide extraction, both at analytical and preparative scales, and available options for peptide detection with MS are discussed. Current algorithms for peptide sequence determination, and both analytical and computational techniques for quantification are compared. Techniques for statistical analysis, sequence mapping, enzyme prediction, and peptide function, and structure prediction are explored.
authors.affiliation: Department of Food Science and Technology, University of California, Davis, One Shields Avenue, Davis, CA, USA; Foods for Health Institute, University of California, Davis, One Shields Avenue, Davis, CA, USA.
authors.forename: David C
authors.initials: DC
authors.lastname: Dallas
authors.forename: Andres
authors.initials: A
authors.lastname: Guerrero
authors.forename: Evan A
authors.initials: EA
authors.lastname: Parker
authors.forename: Randall C
authors.initials: RC
authors.lastname: Robinson
authors.forename: Junai
authors.initials: J
authors.lastname: Gan
authors.forename: J Bruce
authors.initials: JB
authors.lastname: German
authors.forename: Daniela
authors.initials: D
authors.lastname: Barile
authors.forename: Carlito B
authors.initials: CB
authors.lastname: Lebrilla
mesh_terms: D000818:Animals; D015415:Biomarkers; D002417:Cattle; D004044:Dietary Proteins; D004063:Digestion; D005504:Food Analysis; D006801:Humans; D007223:Infant; D013058:Mass Spectrometry; D010446:Peptide Fragments / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D011487:Protein Conformation; D040901:Proteomics / Q000379:methods*; D020539:Sequence Analysis, Protein; D049490:Systems Biology
chemical_list: D015415:Biomarkers; D004044:Dietary Proteins; D010446:Peptide Fragments
keywords: Functional prediction; MS; Peptidomics; Quantitation; Structure prediction; Systems biology