title: Increased membrane lipid peroxidation and decreased Na+/K+-ATPase activity in erythrocytes of patients with stable coronary artery disease.
abstract: OBJECTIVE
  We aimed to determine erythrocyte membrane lipid peroxidation levels and Na/K-ATPase activity in patients with and without coronary artery disease (CAD) documented by coronary angiography.
  
  
  METHODS
  A total of 144 patients who had undergone coronary angiography were divided into a CAD group (n=82) and a non-CAD group (control group, n=62) according to the results of coronary angiography. Lipid peroxide levels in plasma and the erythrocyte membrane were measured using a fluorimetric method. Total antioxidant status and Na/K-ATPase activity in plasma were determined using spectrophotometric methods.
  
  
  RESULTS
  Lipid peroxidation levels were significantly higher in the erythrocyte membrane of CAD patients compared with controls, whereas Na/K-ATPase activity was significantly lower in the erythrocyte membrane of CAD patients compared with controls. The coronary artery scores were correlated positively with membrane lipid peroxidation (r=0.324, P<0.001) and negatively with Na/K-ATPase activity (r=-0.302, P<0.001).
  
  
  CONCLUSIONS
  This study shows that the levels of membrane lipid peroxidation and Na/K-ATPase activity are correlated with the severity of CAD.
authors.affiliation: aDepartment of Clinical Biochemistry, School of Pharmacy and Pharmaceutical Sciences bIsfahan Cardiovascular Research Center cCardiac Rehabilitation Research Center, Isfahan Cardiovascular Research Institute, Isfahan University of Medical Sciences, Isfahan, Iran.
authors.forename: Gholamreza
authors.initials: G
authors.lastname: Namazi
authors.forename: Sabieh
authors.initials: S
authors.lastname: Jamshidi Rad
authors.forename: Ahmad Movahedian
authors.initials: AM
authors.lastname: Attar
authors.forename: Nizal
authors.initials: N
authors.lastname: Sarrafzadegan
authors.forename: Masoumeh
authors.initials: M
authors.lastname: Sadeghi
authors.forename: Gholamali
authors.initials: G
authors.lastname: Naderi
authors.forename: Morteza
authors.initials: M
authors.lastname: Pourfarzam
mesh_terms: D000975:Antioxidants / Q000378:metabolism; D015415:Biomarkers / Q000097:blood; D016022:Case-Control Studies; D017023:Coronary Angiography; D003324:Coronary Artery Disease / Q000097:blood / Q000000981:diagnostic imaging / Q000201:enzymology*; D015536:Down-Regulation; D004910:Erythrocyte Membrane / Q000201:enzymology*; D005260:Female; D006801:Humans; D007492:Iran; D015227:Lipid Peroxidation; D008297:Male; D008563:Membrane Lipids / Q000378:metabolism*; D008875:Middle Aged; D012720:Severity of Illness Index; D000254:Sodium-Potassium-Exchanging ATPase / Q000097:blood*; D013050:Spectrometry, Fluorescence
chemical_list: D000975:Antioxidants; D015415:Biomarkers; D008563:Membrane Lipids; D000254:Sodium-Potassium-Exchanging ATPase