title: Green rapid biogenic synthesis of bioactive silver nanoparticles (AgNPs) using Pseudomonas aeruginosa.
abstract: The present work was focused on isolating a bacterial strain of Pseudomonas sp. with the ability to synthesise AgNPs rapidly. A strain of Pseudomonas aeruginosa designated JO was found to be a potential candidate for rapid synthesis of AgNPs with a synthesis time of 4h in light, at room temperature which is a shorter time period noticed for the synthesis when compared to the previous reports Biosynthesis of AgNPs was achieved by addition of culture supernatant with aqueous silver nitrate solution (1 mM). The reaction mixture exhibits change in colour from green to brown with a peak at 420 nm corresponding to the plasmon absorbance of AgNPs by UV-vis spectroscopy. The nanoparticles were characterised by X-ray diffraction (XRD), energy-dispersive X-ray analysis, Fourier-transform infrared spectroscopy, scanning electron microscopy (SEM), Zetasizer and transmission electron microscopy (TEM). The XRD spectrum exhibited 2θ values corresponding to the silver nanocrystals. TEM and SEM micrographs revealed the extracellular formation of polydispersed elongated nanoparticles with an average size of 27.5 nm. Synthesised nanoparticles showed antibacterial property against both gram-positive and gram-negative microorganisms, but more effective towards gram-negative.
authors.affiliation: Centre for Microbiology, School of Life Sciences, Pondicherry University, Puducherry 605014, India. siddhardha.busi@gmail.com.
authors.forename: Siddhardha
authors.initials: S
authors.lastname: Busi
authors.affiliation: Centre for Microbiology, School of Life Sciences, Pondicherry University, Puducherry 605014, India.
authors.forename: Jobina
authors.initials: J
authors.lastname: Rajkumari
authors.affiliation: Centre for Microbiology, School of Life Sciences, Pondicherry University, Puducherry 605014, India.
authors.forename: Bibhuti
authors.initials: B
authors.lastname: Ranjan
authors.affiliation: Centre for Microbiology, School of Life Sciences, Pondicherry University, Puducherry 605014, India.
authors.forename: Sukumar
authors.initials: S
authors.lastname: Karuganti
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology; D001419:Bacteria / Q000187:drug effects; D019149:Bioreactors / Q000382:microbiology*; D053768:Metal Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D011550:Pseudomonas aeruginosa / Q000235:genetics / Q000378:metabolism*; D012834:Silver / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D012834:Silver