title: Efficient single-cell poration by microsecond laser pulses.
abstract: Payloads including FITC-Dextran dye and plasmids were delivered into NIH/3T3 fibroblasts using microbubbles produced by microsecond laser pulses to induce pores in the cell membranes. Two different operational modes were used to achieve molecular delivery. Smaller molecules, such as the FITC-Dextran dye, were delivered via a scanning-laser mode. The poration efficiency and the cell viability were both 95.1 ± 3.0%. Relatively larger GFP plasmids can be delivered efficiently via a fixed-laser mode, which is a more vigorous method that can create larger transient pores in the cell membrane. The transfection efficiency of 5.7 kb GFP plasmid DNA can reach to 86.7 ± 3.3%. Using this cell poration system, targeted single cells can be porated with high resolution, and cells can be porated in arbitrary patterns.
authors.affiliation: Department of Mechanical Engineering, University of Hawaii at Manoa, 2540 Dole Street, Holmes Hall 302, Honolulu, USA. fanqihui@hawaii.edu.
authors.forename: Qihui
authors.initials: Q
authors.lastname: Fan
authors.forename: Wenqi
authors.initials: W
authors.lastname: Hu
authors.forename: Aaron T
authors.initials: AT
authors.lastname: Ohta
mesh_terms: D000818:Animals; D002470:Cell Survival; D003911:Dextrans / Q000737:chemistry; D016650:Fluorescein-5-isothiocyanate / Q000031:analogs & derivatives / Q000737:chemistry; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D007834:Lasers; D051379:Mice; D008856:Microscopy, Fluorescence; D041681:NIH 3T3 Cells; D010957:Plasmids / Q000235:genetics / Q000378:metabolism*; D013997:Time Factors; D014162:Transfection / Q000379:methods*
chemical_list: D003911:Dextrans; C015219:fluorescein isothiocyanate dextran; D049452:Green Fluorescent Proteins; D016650:Fluorescein-5-isothiocyanate