title: Ashitaba (Angelica keiskei) extract prevents adiposity in high-fat diet-fed C57BL/6 mice.
abstract: Two main chalcones, 4-hydroxyderricin and xanthoangelol, from Ashitaba, which is a food ingredient and a folk medicine in Asia, have been demonstrated to modulate lipid metabolism in 3T3-L1 and HepG2 cells. In this study, we investigated the effects of Ashitaba extract on adiposity in mice fed a high-fat (HF) diet and its underlying mechanisms based on adipose tissue and hepatic lipid metabolism. C57BL/6 mice were fed a normal or HF diet supplemented with Ashitaba extract (0.01% and 0.1%, w/w) for 16 weeks. Ashitaba extract suppressed the HF diet-induced body weight gain and fat deposition in white adipose tissue, reduced plasma cholesterol, glucose, and insulin levels, increased the adiponectin level, lowered triglyceride and the liver cholesterol content, increased phosphorylation of AMP-activated protein kinase (AMPK) in adipose tissue and liver, inhibited lipogenesis in adipose tissue by down-expression of peroxisome proliferator-activated receptor (PPAR) γ, CCAAT/enhancer-binding protein α and sterol regulatory element-binding protein 1 (SREBP1), inhibited lipogenesis in the liver by down-expression of SREBP1 and its target enzyme fatty acid synthase, and promoted fatty acid oxidation by up-expression of carnitine palmitoyltransferase-1A and PPARα. In conclusion, Ashitaba extract can possibly prevent adiposity through modulating lipid metabolism through phosphorylation of AMPK in adipose tissue and liver.
authors.affiliation: Department of Agrobioscience, Graduate School of Agricultural Science, Kobe University, Kobe 657-8501, Japan. ashida@kobe-u.ac.jp.
authors.forename: Tianshun
authors.initials: T
authors.lastname: Zhang
authors.forename: Yoko
authors.initials: Y
authors.lastname: Yamashita
authors.forename: Michiko
authors.initials: M
authors.lastname: Yasuda
authors.forename: Norio
authors.initials: N
authors.lastname: Yamamoto
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Ashida
mesh_terms: D055372:AMP-Activated Protein Kinases / Q000737:chemistry / Q000378:metabolism; D052436:Adipose Tissue, White / Q000201:enzymology / Q000378:metabolism; D050154:Adiposity; D029969:Angelica / Q000737:chemistry*; D000818:Animals; D019440:Anti-Obesity Agents / Q000008:administration & dosage / Q000737:chemistry / Q000627:therapeutic use*; D059305:Diet, High-Fat / Q000009:adverse effects; D019587:Dietary Supplements / Q000032:analysis; D004789:Enzyme Activation; D050155:Lipogenesis; D008099:Liver / Q000201:enzymology / Q000378:metabolism; D008297:Male; D008810:Mice, Inbred C57BL; D009765:Obesity / Q000209:etiology / Q000517:prevention & control*; D010766:Phosphorylation; D010936:Plant Extracts / Q000008:administration & dosage / Q000737:chemistry / Q000627:therapeutic use*; D011499:Protein Processing, Post-Translational; D011897:Random Allocation; D015430:Weight Gain
chemical_list: D019440:Anti-Obesity Agents; D010936:Plant Extracts; D055372:AMP-Activated Protein Kinases