title: Effect of demineralized bone particle/poly(lactic-co-glycolic acid) scaffolds on the attachment and proliferation of mesenchymal stem cells.
abstract: The aim of this study was to investigate the effect of demineralized bone particle/ poly(lactic-co-glycolic acid) (DBP/PLGA) scaffolds on the proliferation of mesenchymal stem cells (MSCs). DBP/PLGA hybrid scaffolds were fabricated by solvent casting/salt-leaching with DBP contents of 0, 20, 40, and 80 wt%. MSCs were seeded on the DBP/PLGA scaffolds and then evaluated by a series of analytical process: SEM, MTT, RT-PCR, and in vivo histological assay. As the DBP contents increased, the cell attachment behavior and cell viability also increased. A DBP content of 80 wt% marked the best water absorption performance and the highest cell viability. Gene expression of aggrecan on DBP/PLGA scaffolds tended to increase, whereas that on PLGA scaffolds was decreased at 1 week. However, strong expression of aggrecan was observed at 2 weeks regardless of the contents of DBP. Scaffolds showed a trend of increasing type II and I collagen at 2 weeks. The results showed that MSCs on DBP/PLGA scaffolds showed more efficient cell proliferation and tissue formation in the presence of tissue-inductive stimuli. Suitable biomaterials could be more conducive to proliferation of MSCs. These results suggest that the DBP/PLGA scaffolds are a feasible biomaterial for intervertebral disc regeneration.
authors.affiliation: a Chonbuk National University Medical School , Jeonbuk 561-180 , South Korea.
authors.forename: Kap-Soo
authors.initials: KS
authors.lastname: Han
authors.forename: Jeong Eun
authors.initials: JE
authors.lastname: Song
authors.forename: Su Ji
authors.initials: SJ
authors.lastname: Kang
authors.forename: Dongwon
authors.initials: D
authors.lastname: Lee
authors.forename: Gilson
authors.initials: G
authors.lastname: Khang
mesh_terms: D053674:Aggrecans / Q000378:metabolism; D000818:Animals; D001672:Biocompatible Materials / Q000138:chemical synthesis / Q000737:chemistry; D018775:Bone Demineralization Technique; D002417:Cattle; D002448:Cell Adhesion; D049109:Cell Proliferation; D002470:Cell Survival; D002478:Cells, Cultured; D024042:Collagen Type I / Q000378:metabolism; D024043:Collagen Type II / Q000378:metabolism; D005260:Female; D015870:Gene Expression; D006801:Humans; D019344:Lactic Acid / Q000138:chemical synthesis / Q000737:chemistry*; D008422:Materials Testing; D059630:Mesenchymal Stem Cells / Q000502:physiology* / Q000648:ultrastructure; D011100:Polyglycolic Acid / Q000138:chemical synthesis / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D017208:Rats, Wistar; D054457:Tissue Scaffolds; D014867:Water / Q000737:chemistry
chemical_list: D053674:Aggrecans; D001672:Biocompatible Materials; D024042:Collagen Type I; D024043:Collagen Type II; D014867:Water; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid
keywords: demineralized bone particle; intervertebral disc; mesenchymal stem cells; poly(lactide-co-glycolide); proliferation