title: Rice stripe tenuivirus p2 may recruit or manipulate nucleolar functions through an interaction with fibrillarin to promote virus systemic movement.
abstract: Rice stripe virus (RSV) is the type species of the genus Tenuivirus and represents a major viral pathogen affecting rice production in East Asia. In this study, RSV p2 was fused to yellow fluorescent protein (p2-YFP) and expressed in epidermal cells of Nicotiana benthamiana. p2-YFP fluorescence was found to move to the nucleolus initially, but to leave the nucleolus for the cytoplasm forming numerous distinct bright spots there at later time points. A bimolecular fluorescence complementation (BiFC) assay showed that p2 interacted with fibrillarin and that the interaction occurred in the nucleus. Both the nucleolar localization and cytoplasmic distribution of p2-YFP fluorescence were affected in fibrillarin-silenced N. benthamiana. Fibrillarin depletion abolished the systemic movement of RSV, but not that of Tobacco mosaic virus (TMV) and Potato virus X (PVX). A Tobacco rattle virus (TRV)-based virus-induced gene silencing (VIGS) method was used to diminish RSV NS2 (encoding p2) or NS3 (encoding p3) during RSV infection. Silencing of NS3 alleviated symptom severity and reduced RSV accumulation, but had no obvious effects on virus movement and the timing of symptom development. However, silencing of NS2 abolished the systemic movement of RSV. The possibility that RSV p2 may recruit or manipulate nucleolar functions to promote virus systemic infection is discussed.
authors.affiliation: Institute of Plant Virology, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
authors.forename: Luping
authors.initials: L
authors.lastname: Zheng
authors.affiliation: Institute of Plant Virology, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.|Guangdong Provincial Key Laboratory of High Technology for Plant Protection, Plant Protection Research Institute, GAAS, Guangzhou, 510640, China.
authors.forename: Zhenguo
authors.initials: Z
authors.lastname: Du
authors.affiliation: Institute of Plant Virology, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.|Bayuquan Entry-Exit Inspection and Quarantine Bureau, Yingkou, 115007, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Lin
authors.affiliation: Institute of Plant Virology, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
authors.forename: Qianzhuo
authors.initials: Q
authors.lastname: Mao
authors.affiliation: Institute of Plant Virology, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
authors.forename: Kangcheng
authors.initials: K
authors.lastname: Wu
authors.affiliation: Institute of Plant Virology, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
authors.forename: Jianguo
authors.initials: J
authors.lastname: Wu
authors.affiliation: Institute of Plant Virology, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
authors.forename: Taiyun
authors.initials: T
authors.lastname: Wei
authors.affiliation: Institute of Plant Virology, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
authors.forename: Zujian
authors.initials: Z
authors.lastname: Wu
authors.affiliation: Institute of Plant Virology, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
authors.forename: Lianhui
authors.initials: L
authors.lastname: Xie
mesh_terms: D002466:Cell Nucleolus / Q000378:metabolism / Q000821:virology*; D002868:Chromosomal Proteins, Non-Histone / Q000378:metabolism*; D010940:Plant Proteins / Q000378:metabolism*; D012191:Retroviridae Proteins / Q000378:metabolism*; D029364:Tenuivirus / Q000502:physiology*; D014026:Nicotiana / Q000821:virology
chemical_list: D002868:Chromosomal Proteins, Non-Histone; D010940:Plant Proteins; D012191:Retroviridae Proteins; C047458:fibrillarin
keywords: Rice stripe virus; fibrillarin; nucleolus; systemic movement