title: Soybean dihydrolipoamide dehydrogenase (ferric leghemoglobin reductase 2) interacts with and reduces ferric rice non-symbiotic hemoglobin 1.
abstract: Ferrous oxygenated hemoglobins (Hb2+O2) autoxidize to ferric Hb3+, but Hb3+is reduced to Hb2+by enzymatic and non-enzymatic mechanisms. We characterized the interaction between the soybean ferric leghemoglobin reductase 2 (FLbR2) and ferric rice non-symbiotic Hb1 (Hb13+). Spectroscopic analysis showed that FLbR2 reduces Hb13+. Analysis by tryptophan fluorescence quenching showed that FLbR2 interacts with Hb13+, however the use of ITC and IEF techniques revealed that this interaction is weak.In silicomodeling showed that predicted FLbR2 and native Hb13+interact at the FAD-binding domain of FLbR2 and the CD-loop and helix F of Hb13+.
authors.affiliation: Lab. de Biofísica y Biología Molecular, Universidad Autónoma del Estado de Morelos, Ave. Universidad 1001 Col. Chamilpa, 62210 Cuernavaca, Morelos, México.
authors.forename: Sabarinathan K
authors.initials: SK
authors.lastname: Gopalasubramaniam
authors.affiliation: Dept. of Biochemistry and Molecular Biology, School of Medicine, Wayne State University, Detroit, Michigan 48201, USA.
authors.forename: Kalyan C
authors.initials: KC
authors.lastname: Kondapalli
authors.affiliation: Fac. de Ciencias, Universidad Autónoma del Estado de Morelos, Ave. Universidad 1001, Col. Chamilpa, 62210 Cuernavaca, Morelos, México.
authors.forename: César
authors.initials: C
authors.lastname: Millán-Pacheco
authors.affiliation: Fac. de Ciencias, Universidad Autónoma del Estado de Morelos, Ave. Universidad 1001, Col. Chamilpa, 62210 Cuernavaca, Morelos, México.
authors.forename: Nina
authors.initials: N
authors.lastname: Pastor
authors.affiliation: Dept. of Biochemistry and Molecular Biology, School of Medicine, Wayne State University, Detroit, Michigan 48201, USA.
authors.forename: Timothy L
authors.initials: TL
authors.lastname: Stemmler
authors.affiliation: Depto. de Ciencias del Medio Natural/Dept. of Environmental Sciences, Public University of Navarre, Spain.
authors.forename: Jose F
authors.initials: JF
authors.lastname: Moran
authors.affiliation: Lab. de Biofísica y Biología Molecular, Universidad Autónoma del Estado de Morelos, Ave. Universidad 1001 Col. Chamilpa, 62210 Cuernavaca, Morelos, México.
authors.forename: Raúl
authors.initials: R
authors.lastname: Arredondo-Peter
keywords: Fluorescence quenching; Hemoglobin; Isothermal calorimetry; Non-symbiotic; Oryza sativa; Protein-protein interaction