title: Dot1L mediated histone H3 lysine79 methylation is essential to meiosis progression in mouse oocytes.
abstract: OBJECTIVE
  Post-translational modifications of lysine residues of histones can result in a series of functional changes. Lysine 79 of histone H3 (H3K79) can be methylated specifically by the Dot1 family of histone lysine methyltransferases. Although multiple developmental abnormalities in Dot1L-deficient mouse embryos have been studied, the biological function of H3K79 methylation in mammal oocytes remains unclear. Here, the distribution of Dot1L, methyltransferase of residue lys79 of histone H3 (H3K79) in mouse, and its effect on mouse oocytes meiosis were investigated to examine whether there are changes in the pattern of distribution and effect of Dot1L on mouse oocytes meiosis.
  
  
  METHODS
  The mRNA level of Dot1l in mouse oocytes was examined using real-time qPCR (RT-qPCR) technique. The distribution and level of Dot1L protein and H3K79 methylation were examined using immunofluorescence and western-blot techniques, respectively. The down regulation of Dot1l in mouse oocytes was conducted using siRNA injection technique.
  
  
  RESULTS
  Dot1L was detected diffuse staining in the nuclear of mouse GV (Germinal Vesicle) stage oocytes. The Dot1l expression and H3K79 methylation level were suppressed effectively with anti-Dot1l siRNA injection. In Dot1L deficient, accompanying with BubR1 (MAD3/Bub1b) remains on the chromosome, the mouse oocytes was blocked in metaphase of meiosis I. The histone deacetylation was also incomplete in Dot1L-deficient mouse oocytes.
  
  
  CONCLUSIONS
  Dot1L protein is well distributed in mouse GV stage oocytes. Dot1L and H3K79 methylation play important roles in meiosis progression and are supposed to be associated with chromosome deacetylation of mouse oocytes.
authors.affiliation: College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, China.
authors.forename: Xuguang
authors.initials: X
authors.lastname: Wang
authors.affiliation: College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, China.
authors.forename: Wenlong
authors.initials: W
authors.lastname: Gao
authors.affiliation: College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, China.
authors.forename: Xueshan
authors.initials: X
authors.lastname: Ma
authors.affiliation: College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, China.
authors.forename: Xiaona
authors.initials: X
authors.lastname: Wang
authors.affiliation: College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, China.
authors.forename: Chunlei
authors.initials: C
authors.lastname: Song
authors.affiliation: College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, China.
authors.forename: Xianju
authors.initials: X
authors.lastname: Huang
authors.affiliation: College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, China.
authors.forename: Honglin
authors.initials: H
authors.lastname: Liu
mesh_terms: D000107:Acetylation; D000818:Animals; D005260:Female; D011495:Histone-Lysine N-Methyltransferase; D006657:Histones / Q000235:genetics / Q000378:metabolism*; D008540:Meiosis / Q000502:physiology*; D008745:Methylation; D008780:Methyltransferases / Q000235:genetics / Q000378:metabolism*; D008813:Mice, Inbred ICR; D009865:Oocytes / Q000166:cytology / Q000378:metabolism*; D011499:Protein Processing, Post-Translational / Q000502:physiology; D012333:RNA, Messenger / Q000235:genetics; D034741:RNA, Small Interfering / Q000235:genetics
chemical_list: D006657:Histones; D012333:RNA, Messenger; D034741:RNA, Small Interfering; C481527:Dot1l protein, mouse; D008780:Methyltransferases; D011495:Histone-Lysine N-Methyltransferase