title: [Live cell fluorescent imaging and cytotoxicity assessment of pH fluorescent probe based on styrylcyanine dyes].
abstract: OBJECTIVE
  To prepare a pH fluorescence probe based on styrylcyanine dyes for live cell imaging.
  
  
  METHODS
  The Probe 1 was prepared by reaction of 4-pyridinecarboxaldehyde with 1,1,2-trimethylbenz[e]indole. The influence of pH on the fluorescent properties was examined, and the cell viability was examined using cell counting kit-8. The Probe 1 was used as a pH fluorescence probe in living cell.
  
  
  RESULTS
  Probe 1 emitted green fluorescence under neutral and basic conditions but orange fluorescence under acid condition. Probe 1 selectively stained the cytoplasmic regions of living cells without significantly affecting the cell viability.
  
  
  CONCLUSIONS
  The pH-sensitive fluorescent probe prepared based on styrylcyanine possesses good ability of cell membrane permeation for live cell fluorescent imaging.
authors.affiliation: Medical Imaging Center, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. E-mail: scroll561@163.com.
authors.forename: Lixia
authors.initials: L
authors.lastname: Huang
authors.forename: Xiang
authors.initials: X
authors.lastname: Liu
authors.forename: Danting
authors.initials: D
authors.lastname: Cui
authors.forename: Yikai
authors.initials: Y
authors.lastname: Xu
authors.forename: Wenwei
authors.initials: W
authors.lastname: You
authors.forename: Yichen
authors.initials: Y
authors.lastname: Yan
authors.forename: Xinwei
authors.initials: X
authors.lastname: Lu
authors.forename: Ruiyuan
authors.initials: R
authors.lastname: Liu
mesh_terms: D002478:Cells, Cultured; D005453:Fluorescence; D005456:Fluorescent Dyes; D006863:Hydrogen-Ion Concentration; D061848:Optical Imaging
chemical_list: D005456:Fluorescent Dyes