title: Neuroprotective effect of Shenfu Injection () following cardiac arrest in pig correlates with improved mitochondrial function and cerebral glucose uptake.
abstract: OBJECTIVE
  To test whether Shenfu Injection (, SFI) might attenuate the impact of cerebral energy dysfunction after resuscitation in a pig model of cardiac arrest (CA).
  
  
  METHODS
  Thirty-four Wuzhishan miniature inbred pigs were randomly divided into three groups: the SFI group (n=12), the saline group (SA group, n=12), and the sham-operated group (sham group, n=10). Following successful return of spontaneous circulation (ROSC) from 8-min untreated ventricular fibrillation, animals received a continuous infusion of either SFI (0.2 mL/min) or saline for 6 h. Cerebral performance category score was evaluated at 24 and 48 h after ROSC, followed by positron emission tomography and computed tomography scans of cerebral glucose uptake. Surviving pigs were euthanized 48 h after ROSC, and the brains were removed for detecting mitochondrial function.
  
  
  RESULTS
  Compared with the SA group, SFI treatment produced a better neurologic outcome 48 h after ROSC (P<0.05). However, there was no significant difference of survival rate between the SA and SFI groups (83.3% vs. 81.8%, P>0.05). After ROSC, the SA group showed a decrease in the maximum standardized uptake value of different regions in the brain tissue, where SFI treatment can ameliorate these decreases (P<0.01 or P<0.05). Improved mitochondrial respiratory properties and higher mitochondrial membrane potential were also found following SFI treatment compared with the SA group at 48 h after ROSC (P<0.05 or P<0.01).
  
  
  CONCLUSIONS
  SFI treatment after resuscitation has significant neuroprotective effects against disruption of cerebral energy metabolism from CA by improving glucose uptake and by normalizing mitochondrial function.
authors.affiliation: Department of Hyperbaric Oxygen, Beijing Chao-Yang Hospital, Capital Medical University, Beijing, 100020, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhang
authors.forename: Chun-Sheng
authors.initials: CS
authors.lastname: Li
authors.forename: Cai-Jun
authors.initials: CJ
authors.lastname: Wu
authors.forename: Jun
authors.initials: J
authors.lastname: Yang
authors.forename: Chen-Chen
authors.initials: CC
authors.lastname: Hang
mesh_terms: D000818:Animals; D001921:Brain / Q000000981:diagnostic imaging / Q000378:metabolism*; D016887:Cardiopulmonary Resuscitation; D004365:Drugs, Chinese Herbal / Q000627:therapeutic use*; D006323:Heart Arrest / Q000188:drug therapy*; D008297:Male; D008928:Mitochondria / Q000502:physiology*; D018696:Neuroprotective Agents / Q000627:therapeutic use*; D049268:Positron-Emission Tomography; D013552:Swine; D013556:Swine, Miniature; D014057:Tomography, X-Ray Computed
chemical_list: D004365:Drugs, Chinese Herbal; D018696:Neuroprotective Agents; C492793:Shen-Fu