title: Nanoemulsions as an effective medium for encapsulation and stabilization of cholesterol/β-cyclodextrin inclusion complex.
abstract: BACKGROUND
  Inclusion complex formation between β-cyclodextrin (β-CD) and suitable guest molecules such as cholesterol (Ch) has regularly been exploited to design self-assembled structures. In the present study an effective nanoemulsion medium (lecithin/Tween 80/ethyl oleate/water) was selected for solubilizing and stabilizing Ch and Ch/β-CD inclusion complex. Phase solubility, spectroscopic, thermodynamic, Z-average diameter and morphological analyses were conducted.
  
  
  RESULTS
  Phase solubility data analysis demonstrated an increase in Ch solubility at low β-CD concentrations (0.01-0.35 mmol L(-1) ). Transmission electron microscopy and Z-average diameter data indicated the spherical nature of the droplets and confirmed the formation of nanoemulsions with an average size of less than 50 nm. The negative value of ΔG obtained during analysis further indicated that the binding was spontaneous in nature.
  
  
  CONCLUSIONS
  Primarily, this research demonstrates the use of nanoemulsions as a medium in food matrices, instead of water, for hosting Ch in β-CD.
authors.affiliation: Department of Chemistry and Centre of Advanced Studies in Chemistry, Panjab University, Chandigarh 160 014, India.
authors.forename: Khushwinder
authors.initials: K
authors.lastname: Kaur
mesh_terms: D002152:Calorimetry, Differential Scanning; D002214:Capsules; D002784:Cholesterol / Q000737:chemistry*; D016503:Drug Delivery Systems; D004655:Emulsions; D055951:Functional Food; D054709:Lecithins / Q000737:chemistry; D049329:Nanostructures; D009829:Oleic Acids / Q000737:chemistry; D010316:Particle Size; D011136:Polysorbates / Q000737:chemistry; D012995:Solubility; D017550:Spectroscopy, Fourier Transform Infrared; D013816:Thermodynamics; D014867:Water / Q000737:chemistry; D047392:beta-Cyclodextrins / Q000737:chemistry*
chemical_list: D002214:Capsules; D004655:Emulsions; D054709:Lecithins; D009829:Oleic Acids; D011136:Polysorbates; D047392:beta-Cyclodextrins; D014867:Water; D002784:Cholesterol; C031215:betadex; C033180:ethyl oleate
keywords: cholesterol; functional foods; nanoemulsions; solubility; β-cyclodextrin