title: Vitellibacter nionensis sp. nov., isolated from a shallow water hydrothermal vent.
abstract: A novel, Gram-stain-negative, non-motile, rod-shaped yellow bacterium, designated VBW088(T) was isolated from a shallow water hydrothermal vent in Espalamaca in the Azores. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain VBW088(T) clustered with three type strains of species of the genus Vitellibacter and exhibited a sequence similarity of 97.3 % with Vitellibacter soesokkakensis RSSK-12(T). However, strain VBW088(T) and V. soesokkakensis RSSK-12(T) exhibited low DNA-DNA relatedness (12.7±3.5 %). Strain VBW088(T) was positive for catalase and oxidase. Growth occurred at 10-37 °C, with the optimum at 30 °C, and at pH 6.0-8.0 (optimum pH 6.0) and in up to 5 % (w/v) NaCl with optimum growth at 1-2 % (w/v) NaCl. The major fatty acids (>10 %) were iso-C15 : 0 (33.5 %) and iso-C17 : 0 3-OH (32.0 %). The polar lipids detected in strain VBW088(T) consisted of phosphatidylethanolamine, one unidentified aminolipid and three unidentified phospholipids. The DNA G+C content of strain VBW088(T) was 36.7 mol%. On the basis of phylogenetic inference, DNA-DNA relatedness, chemotaxonomic analysis and physiological data, the isolate represents a novel species of the genus Vitellibacter, for which the name Vitellibacter nionensis sp. nov. is proposed, with the type strain as VBW088(T) ( = KCTC 32420(T) = MCC 2354(T)).
authors.affiliation: Biological Oceanography Division, CSIR-National Institute of Oceanography, Dona Paula, Goa 403 004, India.
authors.forename: Raju
authors.initials: R
authors.lastname: Rajasabapathy
authors.affiliation: Biological Oceanography Division, CSIR-National Institute of Oceanography, Dona Paula, Goa 403 004, India.
authors.forename: Chellandi
authors.initials: C
authors.lastname: Mohandass
authors.affiliation: Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, Republic of Korea.
authors.forename: Jung-Hoon
authors.initials: JH
authors.lastname: Yoon
authors.affiliation: NCIM Resource Center, CSIR-National Chemical Laboratory, Dr. Homi Bhabha road, Pune 411 008, India.
authors.forename: Syed Gulam
authors.initials: SG
authors.lastname: Dastager
authors.affiliation: Yunnan Institute of Microbiology, Yunnan University, Kunming, Yunnan 650091, PR China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Liu
authors.affiliation: School of Biotechnology and Food Technology, Hanoi University of Science and Technology, Vietnam.|Yunnan Institute of Microbiology, Yunnan University, Kunming, Yunnan 650091, PR China.
authors.forename: Thi-Nhan
authors.initials: TN
authors.lastname: Khieu
authors.affiliation: School of Biotechnology and Food Technology, Hanoi University of Science and Technology, Vietnam.
authors.forename: Chu Ky
authors.initials: CK
authors.lastname: Son
authors.affiliation: Yunnan Institute of Microbiology, Yunnan University, Kunming, Yunnan 650091, PR China.
authors.forename: Wen-Jun
authors.initials: WJ
authors.lastname: Li
authors.affiliation: IMAR-Centre of IMAR of the University of the Azores/Department of Oceanography and Fisheries and LARSyS Associated Laboratory, Rua Prof Frederico Machado 9901-862, Horta, Portugal.
authors.forename: Ana
authors.initials: A
authors.lastname: Colaço
mesh_terms: D001394:Azores; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D041965:Flavobacteriaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D060086:Hydrothermal Vents / Q000382:microbiology*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D010858:Pigmentation; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S