title: [Preliminary screening of downstream proteins of Sox2 and role of Sox2 in colonic cancer cell migration and invasion].
abstract: OBJECTIVE
  To screen the down-stream proteins of transcription factor Sox2 and explore the role of Sox2 in the proliferation and migration of colonic cancer cells in vitro.
  
  
  METHODS
  The cellular proteins were separated by SDS-PAGE electrophoresis and stained with Coomassie blue and amine plated silver. The differentially expressed proteins was identified by mass spectrometry and verified by QPCR and Western blotting. A cell counting kit-8 (CCK8) assay was performed to evaluate the cell proliferation, and the cell migration was assessed using Transwell assay.
  
  
  RESULTS
  S3a was identified by proteomics technology as a Sox2-downregulated protein while ENO1 and gama-actin the up-regulated proteins. QPCR and Western blotting analyses showed that overexpression of Sox2 significantly decreased the expression of S3a (P<0.005) and increased the expression of ENO1(P<0.05), but had no significant effect on gama-actin expression. Sox2 overexpression obviously promoted cell proliferation and migration (P<0.05), while inhibition of Sox2 produced contrary effects (P<0.05).
  
  
  CONCLUSIONS
  Sox2 negatively regulates S3a expression and positively regulates ENO1 expression to promot the proliferation and migration of colonic cancer cells.
authors.affiliation: Department of Pathology, College of Basic Medical Sciences, Southern Medical University; Key Laboratory of Molecular Tumor Pathology of Guangdong Province, Guangzhou 510515, China. E-mail: minzai5366@126.com.
authors.forename: Min
authors.initials: M
authors.lastname: Zhou
authors.forename: Yanxia
authors.initials: Y
authors.lastname: Lu
authors.forename: Li
authors.initials: L
authors.lastname: Yuan
authors.forename: Lin
authors.initials: L
authors.lastname: Zheng
authors.forename: Yan
authors.initials: Y
authors.lastname: Liu
authors.forename: Min
authors.initials: M
authors.lastname: Hong
authors.forename: Chao
authors.initials: C
authors.lastname: Zhang
authors.forename: Xuenong
authors.initials: X
authors.lastname: Li
mesh_terms: D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D003110:Colonic Neoplasms / Q000378:metabolism*; D015536:Down-Regulation; D006801:Humans; D040901:Proteomics; D012269:Ribosomal Proteins / Q000378:metabolism; D055748:SOXB1 Transcription Factors / Q000378:metabolism*; D015854:Up-Regulation
chemical_list: C031016:RPS3A protein, human; D012269:Ribosomal Proteins; C527782:SOX2 protein, human; D055748:SOXB1 Transcription Factors