title: [Identification of susceptibility gene for pig umbilical hernia in different populations using transmission disequilibrium test].
abstract: A genome-wide scan for pig umbilical hernia (UH) was performed in a White Duroc × Erhualian resource population reported by our previously study, which detected two susceptibility microsatellite markers (SWR1928 on SSC7 and SW830 on SSC10) significantly affecting pig UH. Herein, fine mapping studies and identification of susceptibility genes for UH were performed in two different populations. A total of 40 SNPs in 12 positional candidate genes located on the two significant segments were genotyped in the F2/F3 resource population. Quality control of the genotype data and transmission disequilibrium test (TDT) were conducted using Plink v1.07 software. The results showed that g.708G>A in IL16 (interleukin 16) gene and g.10664G>A in CDC73 (cell division cycle 73) gene were significantly associated with pig UH. These two prominent SNPs and another two weakly associated SNPs g.10546A>G and g.10811A>G in CDC73 were also undergone the replication TDT test in the outbred commercial populations. All SNPs in the CDC73 gene were confirmed to be significantly associated with pig UH (P<0.05), including g. 10546A>G and g.10811A>G with extreme significant level (P<0.01). Based on these results, CDC73 should be a susceptibility gene for pig UH according to its biological functions and the molecular pathogenesis of UH.
authors.affiliation: Candidate of National Key Laboratory for Animal Biotechnology, Jiangxi Agricultural University, Nanchang, 330045, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Su
authors.affiliation: Candidate of National Key Laboratory for Animal Biotechnology, Jiangxi Agricultural University, Nanchang, 330045, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Long
authors.affiliation: 1. Candidate of National Key Laboratory for Animal Biotechnology, Jiangxi Agricultural University, Nanchang, 330045, China; 2. Fujian Vocational College of Agriculture, Fuzhou 350119, China;
authors.forename: Guorong
authors.initials: G
authors.lastname: Ruan
authors.affiliation: Candidate of National Key Laboratory for Animal Biotechnology, Jiangxi Agricultural University, Nanchang, 330045, China.
authors.forename: Lihua
authors.initials: L
authors.lastname: Wu
authors.affiliation: Candidate of National Key Laboratory for Animal Biotechnology, Jiangxi Agricultural University, Nanchang, 330045, China.
authors.forename: Zhiyan
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Candidate of National Key Laboratory for Animal Biotechnology, Jiangxi Agricultural University, Nanchang, 330045, China.
authors.forename: Shijun
authors.initials: S
authors.lastname: Xiao
authors.affiliation: Candidate of National Key Laboratory for Animal Biotechnology, Jiangxi Agricultural University, Nanchang, 330045, China.
authors.forename: Weiyun
authors.initials: W
authors.lastname: Deng
authors.affiliation: Candidate of National Key Laboratory for Animal Biotechnology, Jiangxi Agricultural University, Nanchang, 330045, China.
authors.forename: Xianshan
authors.initials: X
authors.lastname: Lv
authors.affiliation: Candidate of National Key Laboratory for Animal Biotechnology, Jiangxi Agricultural University, Nanchang, 330045, China.
authors.forename: Dou
authors.initials: D
authors.lastname: Hu
authors.affiliation: Candidate of National Key Laboratory for Animal Biotechnology, Jiangxi Agricultural University, Nanchang, 330045, China.
authors.forename: Guozao
authors.initials: G
authors.lastname: Wu
authors.affiliation: Candidate of National Key Laboratory for Animal Biotechnology, Jiangxi Agricultural University, Nanchang, 330045, China.
authors.forename: Huqun
authors.initials: H
authors.lastname: Shen
authors.affiliation: 1. Candidate of National Key Laboratory for Animal Biotechnology, Jiangxi Agricultural University, Nanchang, 330045, China; 2. College of Life Science of Jinggangshan University, Ji'an 343009, China;
authors.forename: Xinjun
authors.initials: X
authors.lastname: Liao
authors.affiliation: Candidate of National Key Laboratory for Animal Biotechnology, Jiangxi Agricultural University, Nanchang, 330045, China.
authors.forename: Nengshui
authors.initials: N
authors.lastname: Ding
authors.affiliation: Candidate of National Key Laboratory for Animal Biotechnology, Jiangxi Agricultural University, Nanchang, 330045, China.
authors.forename: Lusheng
authors.initials: L
authors.lastname: Huang
mesh_terms: D000818:Animals; D001947:Breeding; D002874:Chromosome Mapping; D020022:Genetic Predisposition to Disease / Q000235:genetics*; D060005:Genotyping Techniques; D006554:Hernia, Umbilical / Q000235:genetics / Q000662:veterinary*; D015810:Linkage Disequilibrium; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D013552:Swine / Q000235:genetics*; D013553:Swine Diseases / Q000235:genetics*