title: Volatile profiling of aromatic traditional medicinal plant, Polygonum minus in different tissues and its biological activities.
abstract: The aim of this research was to identify the volatile metabolites produced in different organs (leaves, stem and roots) of Polygonum minus, an important essential oil producing crop in Malaysia. Two methods of extraction have been applied: Solid Phase Microextraction (SPME) and hydrodistillation coupled with Gas Chromatography-Mass Spectrometry (GC-MS). Approximately, 77 metabolites have been identified and aliphatic compounds contribute significantly towards the aroma and flavour of this plant. Two main aliphatic compounds: decanal and dodecanal were found to be the major contributor. Terpenoid metabolites were identified abundantly in leaves but not in the stem and root of this plant. Further studies on antioxidant, total phenolic content, anticholinesterase and antimicrobial activities were determined in the essential oil and five different extracts. The plant showed the highest DPPH radical scavenging activity in polar (ethanol) extract for all the tissues tested. For anti-acetylcholinesterase activity, leaf in aqueous extract and methanol extract showed the best acetylcholinesterase inhibitory activities. However, in microbial activity, the non-polar extracts (n-hexane) showed high antimicrobial activity against Methicillin-resistant Staphylococcus aureus (MRSA) compared to polar extracts. This study could provide the first step in the phytochemical profiles of volatile compounds and explore the additional value of pharmacology properties of this essential oil producing crop Polygonum minus.
authors.affiliation: Metabolomics Research Laboratory, Institute of Systems Biology, Universiti Kebangsaan Malaysia, Bangi 43600, Selangor, Malaysia. fida.ahmad@ukm.edu.my.
authors.forename: Rafidah
authors.initials: R
authors.lastname: Ahmad
authors.affiliation: Metabolomics Research Laboratory, Institute of Systems Biology, Universiti Kebangsaan Malaysia, Bangi 43600, Selangor, Malaysia. nataqain@ukm.edu.my.
authors.forename: Syarul Nataqain
authors.initials: SN
authors.lastname: Baharum
authors.affiliation: Metabolomics Research Laboratory, Institute of Systems Biology, Universiti Kebangsaan Malaysia, Bangi 43600, Selangor, Malaysia. hamidunb@yahoo.com.
authors.forename: Hamidun
authors.initials: H
authors.lastname: Bunawan
authors.affiliation: Department of Bio-Environment Chemistry, Chungnam National University, 99 Daehak-Ro, Yuseong-Gu, Daejon 305-764, Korea. mklove22@nate.com.
authors.forename: Minki
authors.initials: M
authors.lastname: Lee
authors.affiliation: Metabolomics Research Laboratory, Institute of Systems Biology, Universiti Kebangsaan Malaysia, Bangi 43600, Selangor, Malaysia. normah@ukm.edu.my.
authors.forename: Normah
authors.initials: N
authors.lastname: Mohd Noor
authors.affiliation: Metabolomics Research Laboratory, Institute of Systems Biology, Universiti Kebangsaan Malaysia, Bangi 43600, Selangor, Malaysia. emelda@ukm.edu.my.
authors.forename: Emelda Roseleena
authors.initials: ER
authors.lastname: Rohani
authors.affiliation: Metabolomics Research Laboratory, Institute of Systems Biology, Universiti Kebangsaan Malaysia, Bangi 43600, Selangor, Malaysia. ekin86@ukm.edu.my.
authors.forename: Norashikin
authors.initials: N
authors.lastname: Ilias
authors.affiliation: Programme of Biomedical Science, Faculty of Health Sciences, School of Diagnostic and Applied Health Sciences, Universiti Kebangsaan Malaysia, Kuala Lumpur 50300, Malaysia. noraziah.zin@ukm.edu.my.
authors.forename: Noraziah Mohamad
authors.initials: NM
authors.lastname: Zin
mesh_terms: D000890:Anti-Infective Agents / Q000494:pharmacology; D000975:Antioxidants / Q000494:pharmacology; D002800:Cholinesterase Inhibitors / Q000494:pharmacology; D016166:Free Radical Scavengers / Q000494:pharmacology; D008296:Malaysia; D055624:Methicillin-Resistant Staphylococcus aureus / Q000187:drug effects; D008826:Microbial Sensitivity Tests / Q000379:methods; D009822:Oils, Volatile / Q000494:pharmacology*; D010636:Phenols / Q000494:pharmacology; D010936:Plant Extracts / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry; D018517:Plant Roots / Q000737:chemistry; D018547:Plant Stems / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D028502:Polygonum / Q000737:chemistry*; D013729:Terpenes / Q000494:pharmacology
chemical_list: D000890:Anti-Infective Agents; D000975:Antioxidants; D002800:Cholinesterase Inhibitors; D016166:Free Radical Scavengers; D009822:Oils, Volatile; D010636:Phenols; D010936:Plant Extracts; D013729:Terpenes