title: [Expression and significance of bone sialoprotein(BSP) in esophageal squamous cell carcinoma].
abstract: OBJECTIVE
  To investigate the roll of bone sialoprotein (BSP), a secreted glycoprotein, found in mineralized tissues in the development and progression of human esophageal squamous cell carcimoma (ESCC), and explore its association with clinicopathological characteristics and five-year survival of the patients.
  
  
  METHODS
  The expression of BSP was determined in 211 primary ESCC tumors and their paired nontumorous tissues using tissue-array, RT-PCR and immunohistochemistry.
  
  
  RESULTS
  Primary ESCC tissues showed a significantly higher expression rate of BSP mRNA than their paired nontumorous tissues (93.8% vs. 16.6%, P < 0.001), the same with BSP protein (56.9% vs. 31.3%, P < 0.001). The expression rate of BSP protein was correlated to lymph node metastasis and TNM stage (P < 0.05). The 5-year survival rate of BSP protein-positive ESCC patients was significantly lower than that of BSP protein-negative ESCC patients (P < 0.05). Multivariate analysis showed that tumor differentiation, TNM staging and BSP protein expression were independent factors affecting the prognosis of ESCC patients (P < 0.05).
  
  
  CONCLUSIONS
  The abnormal expression of BSP may play a significant role in the malignant progression and prognosis of ESCC, and BSP might be a marker reflecting the biologial behavior of ESCC.
authors.affiliation: Tumor Hospital Affiliated to Zhengzhou University, Zhengzhou 450008, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Tang
authors.forename: Haiying
authors.initials: H
authors.lastname: Wang
authors.forename: Lili
authors.initials: L
authors.lastname: Wang
authors.forename: Qiming
authors.initials: Q
authors.lastname: Wang
authors.forename: Yanru
authors.initials: Y
authors.lastname: Qin
authors.forename: Xianzeng
authors.initials: X
authors.lastname: Wang
authors.affiliation: Email: luosxrm@163.com.
authors.forename: Suxia
authors.initials: S
authors.lastname: Luo
mesh_terms: D015153:Blotting, Western; D002294:Carcinoma, Squamous Cell / Q000175:diagnosis / Q000378:metabolism*; D004938:Esophageal Neoplasms / Q000175:diagnosis / Q000378:metabolism*; D000077277:Esophageal Squamous Cell Carcinoma; D006801:Humans; D007150:Immunohistochemistry; D058574:Integrin-Binding Sialoprotein / Q000235:genetics / Q000378:metabolism*; D008207:Lymphatic Metastasis; D009367:Neoplasm Staging; D011379:Prognosis; D012333:RNA, Messenger; D015996:Survival Rate
chemical_list: D058574:Integrin-Binding Sialoprotein; D012333:RNA, Messenger