title: Recruitment of SH-containing peptides to lipid and biological membranes through the use of a palmitic acid functionalized with a maleimide group.
abstract: This study presents a novel and easily applicable approach to recruit sulfhydryl-containing biomolecules to membranes by using a palmitic acid which is functionalized with a maleimide group. Notably, this strategy can also be employed with preformed (biological) membranes. The applicability of the assay is demonstrated by characterizing the binding of a Rhodamine-labeled peptide to lipid and cellular membranes using methods of fluorescence spectroscopy, lifetime measurement, and microscopy. Our approach offers new possibilities for preparing biologically active liposomes and manipulating living cells.
authors.affiliation: Department of Biology, Humboldt University Berlin, Invalidenstrasse 42, 10115 Berlin (Germany).
authors.forename: Ivan
authors.initials: I
authors.lastname: Haralampiev
authors.forename: Monique
authors.initials: M
authors.lastname: Mertens
authors.forename: Roland
authors.initials: R
authors.lastname: Schwarzer
authors.forename: Andreas
authors.initials: A
authors.lastname: Herrmann
authors.forename: Rudolf
authors.initials: R
authors.lastname: Volkmer
authors.forename: Pablo
authors.initials: P
authors.lastname: Wessig
authors.forename: Peter
authors.initials: P
authors.lastname: Müller
mesh_terms: D002462:Cell Membrane / Q000378:metabolism*; D008081:Liposomes / Q000737:chemistry / Q000378:metabolism*; D008264:Macrophages / Q000166:cytology / Q000378:metabolism; D008301:Maleimides / Q000737:chemistry / Q000378:metabolism*; D018613:Microscopy, Confocal; D019308:Palmitic Acid / Q000737:chemistry / Q000378:metabolism*; D010455:Peptides / Q000737:chemistry / Q000378:metabolism*; D013050:Spectrometry, Fluorescence; D013438:Sulfhydryl Compounds / Q000032:analysis / Q000378:metabolism*
chemical_list: D008081:Liposomes; D008301:Maleimides; D010455:Peptides; D013438:Sulfhydryl Compounds; C043592:maleimide; D019308:Palmitic Acid
keywords: liposomes; maleimide; membranes; palmitic acid; palmitoylation; peptides