title: [Effect of inhibiting brain reactive oxygen species on sympathetic nerve activity in DOCA-salt hypertensive rats].
abstract: OBJECTIVE
  To investigate whether brain reactive oxygen species mediate sympathoexcitation and arterial pressure elevation in DOCA-salt hypertensive rats.
  
  
  METHODS
  DOCA-salt hypertensive model was established in male SD rats by subcutaneous injection of DOCA after uninephrectomy and drinking 1% NaCl solution for 4 weeks. The baseline mean arterial pressure (MAP), heart rate (HR) and renal sympathetic nerve activity (RSNA) were recorded in the rats under mild anesthesia, and MAP changes following intravenous hexamethonium injection were observed. The responses of MAP, HR and RSNA to intracerebroventricular administration of tempol (20 µmol/L in 10 µl) were evaluated; plasma NE level was measured with ELISA, and ROS level and NAD(P)H oxidase activity in the hypothalamus were detected using chemiluminescence assay.
  
  
  RESULTS
  MAP and plasma NE levels were significantly increased in DOCA-salt rats as compared with those in the control group (P<0.01). In DOCA-salt hypertensive rats, intravenous hexamethonium injection induced a blood pressure reduction 240% of that in control rats, and significantly increased the levels of superoxide anion and NAD(P)H oxidase activity in the hypothalamus. Intracerebroventricular microinjection of tempol also resulted in more significant changes of MAP, HR and RSNA in DOCA-salt rats than in the control group (P<0.01).
  
  
  CONCLUSIONS
  Sympathoexcitation due to increased NAD(P)H oxidase-derived ROS levels in the hypothalamus may mediate arterial pressure elevation in DOCA-salt hypertensive rats.
authors.affiliation: Medical Research Experimental Center, Shaanxi University of Chinese Medicine, Xianyang 712046, China. E-mail: zhangqimail@163.com.
authors.forename: Qi
authors.initials: Q
authors.lastname: Zhang
authors.forename: Yingying
authors.initials: Y
authors.lastname: Tan
mesh_terms: D000818:Animals; D000975:Antioxidants; D062186:Arterial Pressure; D001794:Blood Pressure; D001921:Brain / Q000378:metabolism*; D003497:Cyclic N-Oxides / Q000494:pharmacology; D003900:Desoxycorticosterone; D064791:Desoxycorticosterone Acetate; D004195:Disease Models, Animal; D006339:Heart Rate; D006973:Hypertension; D007668:Kidney / Q000294:innervation; D008297:Male; D019255:NADPH Oxidases / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D017382:Reactive Oxygen Species / Q000378:metabolism*; D012965:Sodium Chloride; D013113:Spin Labels; D013481:Superoxides / Q000378:metabolism; D013564:Sympathetic Nervous System
chemical_list: D000975:Antioxidants; D003497:Cyclic N-Oxides; D017382:Reactive Oxygen Species; D013113:Spin Labels; D013481:Superoxides; D003900:Desoxycorticosterone; D012965:Sodium Chloride; D064791:Desoxycorticosterone Acetate; D019255:NADPH Oxidases; C001803:tempol