title: Different inhibitory effect and mechanism of hydroxyapatite nanoparticles on normal cells and cancer cells in vitro and in vivo.
abstract: Hydroxyapatite (HAP), similar to inorganic phase in bones, shows good biocompatibility and bioactivity as bone defect repairing material. Recently, nanoscaled HAP shows the special properties differing from bulk HAP in physics, chemistry and biology. This paper demonstrates that HAP nanoparticle (nHAP) possesses the ability for inhibiting cancer cell growth in vitro and in vivo. In vitro, after treatment with nHAP for 3 days, proliferation of human cancer cells are inhibited by more than 65% and by less than 30% for human normal cells. In vivo, injection of nHAP in transplanted tumor results in significant reduction (about 50%) of tumor size. The anticancer effect of nHAP is mainly attributed to high amount by endocytosis in cancer cells and inhibition on protein synthesis in cells. The abundant nHAP internalized in cancer cells around endoplasmic reticulum may inhibit the protein synthesis by decreasing the binding of mRNA to ribosome due to its high adsorption capacity for ribosome and arrest cell cycle in G0/G1 phase. nHAP shows no ROS-involved cytotoxicity and low cytotoxicity to normal cells. These results strongly suggest that nHAP can inhibit cancer cell proliferation and have a potential application in cancer treatment.
authors.affiliation: Biomedical Materials and Engineering Center, Wuhan University of Technology, 122 Luoshi Road, Wuhan, 430070, P.R. China.
authors.forename: Yingchao
authors.initials: Y
authors.lastname: Han
authors.affiliation: 1] Biomedical Materials and Engineering Center, Wuhan University of Technology, 122 Luoshi Road, Wuhan, 430070, P.R. China [2] State Key Laboratory of Advanced Technology for Materials Synthesis and Processing, 122 Luoshi Road, Wuhan, 430070, P.R. China.
authors.forename: Shipu
authors.initials: S
authors.lastname: Li
authors.affiliation: Biomedical Materials and Engineering Center, Wuhan University of Technology, 122 Luoshi Road, Wuhan, 430070, P.R. China.
authors.forename: Xianying
authors.initials: X
authors.lastname: Cao
authors.affiliation: Biomedical Materials and Engineering Center, Wuhan University of Technology, 122 Luoshi Road, Wuhan, 430070, P.R. China.
authors.forename: Lin
authors.initials: L
authors.lastname: Yuan
authors.affiliation: Biomedical Materials and Engineering Center, Wuhan University of Technology, 122 Luoshi Road, Wuhan, 430070, P.R. China.
authors.forename: Youfa
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Biomedical Materials and Engineering Center, Wuhan University of Technology, 122 Luoshi Road, Wuhan, 430070, P.R. China.
authors.forename: Yixia
authors.initials: Y
authors.lastname: Yin
authors.affiliation: Biomedical Materials and Engineering Center, Wuhan University of Technology, 122 Luoshi Road, Wuhan, 430070, P.R. China.
authors.forename: Tong
authors.initials: T
authors.lastname: Qiu
authors.affiliation: Biomedical Materials and Engineering Center, Wuhan University of Technology, 122 Luoshi Road, Wuhan, 430070, P.R. China.
authors.forename: Honglian
authors.initials: H
authors.lastname: Dai
authors.affiliation: Biomedical Materials and Engineering Center, Wuhan University of Technology, 122 Luoshi Road, Wuhan, 430070, P.R. China.
authors.forename: Xinyu
authors.initials: X
authors.lastname: Wang
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology*; D006528:Carcinoma, Hepatocellular / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D002460:Cell Line; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D017886:Durapatite / Q000737:chemistry / Q000494:pharmacology*; D004705:Endocytosis; D004721:Endoplasmic Reticulum; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D022781:Hepatocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D015552:Injections, Intralesional; D015603:Keratinocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D008113:Liver Neoplasms / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D051379:Mice; D008819:Mice, Nude; D053758:Nanoparticles / Q000737:chemistry / Q000633:toxicity*; D009928:Organ Specificity; D010006:Osteoblasts / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D023041:Xenograft Model Antitumor Assays
chemical_list: D000970:Antineoplastic Agents; D017886:Durapatite