title: Isolation and molecular characterization of an ethylene response factor NtERF1-1 in Nicotiana tabacum cv. Xanthi.
abstract: Apetala2/Ethylene Response Factors (AP2/ERF) play important roles in regulating gene expression under abiotic and biotic stress in the plant kingdom. Here, we isolated a member of the AP2/ERF transcription factors, NtERF1-1, from Nicotiana tabcum cv. Xanthi NN carrying the N gene, which is resistant to Tobacco mosaic virus (TMV). NtERF1-1 encoded a putative protein of 229 amino acids with a predicted molecular mass of 24.58 kDa. Nucleotide sequence analysis showed that NtERF1-1 contained a conserved DNA-binding domain at the N-terminal. Comparison of amino acid sequences revealed that NtERF1-1 possessed high similarities to ERFs from diverse plants. Semi-quantitative and real-time quantitative RT-PCR analyses indicated that NtERF1-1 was up-regulated following TMV infection. In addition, we speculated that NtERF1-1 might participate in the signal transduction pathway of defence response inducted by the interaction between the N gene and TMV.
authors.affiliation: School of Life Sciences, Anhui Agricultural University, 230036 Hefei, Anhui, China.
authors.forename: Jun-Shan
authors.initials: JS
authors.lastname: Gao
authors.forename: Li
authors.initials: L
authors.lastname: Hu
authors.forename: Peng
authors.initials: P
authors.lastname: Xie
authors.forename: Yan
authors.initials: Y
authors.lastname: Meng
authors.forename: Yong-Ping
authors.initials: YP
authors.lastname: Cai
authors.forename: Yi
authors.initials: Y
authors.lastname: Lin
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D001665:Binding Sites; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D060467:Disease Resistance / Q000235:genetics; D018506:Gene Expression Regulation, Plant; D008969:Molecular Sequence Data; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D016415:Sequence Alignment; D020539:Sequence Analysis, Protein; D014026:Nicotiana / Q000235:genetics* / Q000378:metabolism / Q000821:virology; D015854:Up-Regulation
chemical_list: D004268:DNA-Binding Proteins; D010940:Plant Proteins; C093680:ethylene-responsive element binding protein