title: [Prediction and identification of T-cell epitopes in major group 3 allergen derived from Dermatophagoides farina].
abstract: OBJECTIVE
  To predict and identify T cell epitopes of major group 3 allergen derived from Dermatophagoidesfarina (Der f 3).
  
  
  METHODS
  The T cell epitopes of Der f 3 were analyzed through the sequence analysis by using the bioinformatics online tools. The five predicted peptides of T-cell epitopes were artificially synthesized. The spleen lymphocytes were co-cultured with the five T cell epitopes by using the modified MTT method and the levels of IL-2, IFN-γ, IL-4 and IL-5 in the supernatant of the cultures were detected by ELISA.
  
  
  RESULTS
  Five T cell epitopes of Der f 3 were predicted and three of which could promote the proliferation of the mouse spleen lymphocytes. The secretions of IL-2 and IFN-γ were significantly induced and the secretions of IL-4 and IL-5 were significantly decreased by three of five prediction epitopes of Der f 3: 37GDCPYQISLQSSSHFCGG54, 98IYQHENYDSMTIDNDVALIKLKTPMT123 and 164SELQRVDIDVVSREQCDQLYS184.
  
  
  CONCLUSIONS
  Three T cell epitopes of Der f 3 have been initially identified, which lays the foundation of the diagnosis and treatment of asthma.
authors.forename: Na
authors.initials: N
authors.lastname: Li
authors.forename: Chao-pin
authors.initials: CP
authors.lastname: Li
authors.forename: Ji-dong
authors.initials: JD
authors.lastname: Diao
authors.forename: Bei-bei
authors.initials: BB
authors.lastname: Zhao
authors.forename: Yu-xin
authors.initials: YX
authors.lastname: Jiang
mesh_terms: D000818:Animals; D039741:Antigens, Dermatophagoides / Q000276:immunology*; D049109:Cell Proliferation; D018604:Epitope Mapping; D018984:Epitopes, T-Lymphocyte / Q000276:immunology*; D039981:Pyroglyphidae / Q000276:immunology*; D013154:Spleen / Q000276:immunology; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology
chemical_list: D039741:Antigens, Dermatophagoides; D018984:Epitopes, T-Lymphocyte