title: A spatially and chemically defined platform for the uniform growth of human pluripotent stem cells.
abstract: We report the design of a chemically defined platform engineered for the culture of human pluripotent stem cells (hPSCs) that supports the long-term maintenance of self-renewing hPSC populations in a more uniform manner than standard culture systems. Microcontact printing (μCP) of alkanethiol self-assembled monolayers (SAMs) was used to spatially direct hPSC adherence. This technique not only establishes control over hPSC colony size and shape but also preserves genetic stability and provides unprecedented uniformity in the pluripotency of hPSC populations that is quantitatively assessed in the present study.
authors.affiliation: Department of Materials Science and Engineering, University of California Los Angeles, Los Angeles, California 90095, USA.
authors.forename: Steven J
authors.initials: SJ
authors.lastname: Jonas
authors.affiliation: Department of Microbiology, Immunology, and Molecular Genetics, University of California Los Angeles, Los Angeles, California 90095, United States.
authors.forename: Jackelyn A
authors.initials: JA
authors.lastname: Alva
authors.affiliation: Department of Materials Science and Engineering, University of California Los Angeles, Los Angeles, California 90095, United States.
authors.forename: Wade
authors.initials: W
authors.lastname: Richardson
authors.affiliation: Molecular Biology Institute, University of California Los Angeles, Los Angeles, California 90095, United States.
authors.forename: Sean P
authors.initials: SP
authors.lastname: Sherman
authors.affiliation: Department of Microbiology, Immunology, and Molecular Genetics, University of California Los Angeles, Los Angeles, California 90095, United States; Eli & Edythe Broad Center of Regenerative Medicine & Stem Cell Research, University of California Los Angeles, Los Angeles, California 90095, United States.
authors.forename: Zoran
authors.initials: Z
authors.lastname: Galic
authors.affiliation: Department of Microbiology, Immunology, and Molecular Genetics, University of California Los Angeles, Los Angeles, California 90095, United States; Molecular Biology Institute, University of California Los Angeles, Los Angeles, California 90095, United States; Eli & Edythe Broad Center of Regenerative Medicine & Stem Cell Research, University of California Los Angeles, Los Angeles, California 90095, United States. Electronic address: apyle@mednet.ucla.edu.
authors.forename: April D
authors.initials: AD
authors.lastname: Pyle
authors.affiliation: Department of Materials Science and Engineering, University of California Los Angeles, Los Angeles, California 90095, United States; Eli & Edythe Broad Center of Regenerative Medicine & Stem Cell Research, University of California Los Angeles, Los Angeles, California 90095, United States; California NanoSystems Institute, University of California Los Angeles, Los Angeles, California 90095, United States. Electronic address: bdunn@ucla.edu.
authors.forename: Bruce
authors.initials: B
authors.lastname: Dunn
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry* / Q000494:pharmacology; D002448:Cell Adhesion / Q000187:drug effects; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D003094:Collagen / Q000737:chemistry; D004129:Dimethylpolysiloxanes / Q000737:chemistry; D004338:Drug Combinations; D053595:Embryonic Stem Cells / Q000166:cytology / Q000378:metabolism; D006801:Humans; D007621:Karyotyping; D007797:Laminin / Q000737:chemistry; D039904:Pluripotent Stem Cells / Q000166:cytology* / Q000378:metabolism; D011509:Proteoglycans / Q000737:chemistry; D012825:Silicon / Q000737:chemistry; D014157:Transcription Factors / Q000378:metabolism
chemical_list: D001672:Biocompatible Materials; D004129:Dimethylpolysiloxanes; D004338:Drug Combinations; D007797:Laminin; D011509:Proteoglycans; D014157:Transcription Factors; C059485:matrigel; C013830:baysilon; D003094:Collagen; D012825:Silicon