title: Human Staufen1 associates to miRNAs involved in neuronal cell differentiation and is required for correct dendritic formation.
abstract: Double-stranded RNA-binding proteins are key elements in the intracellular localization of mRNA and its local translation. Staufen is a double-stranded RNA binding protein involved in the localised translation of specific mRNAs during Drosophila early development and neuronal cell fate. The human homologue Staufen1 forms RNA-containing complexes that include proteins involved in translation and motor proteins to allow their movement within the cell, but the mechanism underlying translation repression in these complexes is poorly understood. Here we show that human Staufen1-containing complexes contain essential elements of the gene silencing apparatus, like Ago1-3 proteins, and we describe a set of miRNAs specifically associated to complexes containing human Staufen1. Among these, miR-124 stands out as particularly relevant because it appears enriched in human Staufen1 complexes and is over-expressed upon differentiation of human neuroblastoma cells in vitro. In agreement with these findings, we show that expression of human Staufen1 is essential for proper dendritic arborisation during neuroblastoma cell differentiation, yet it is not necessary for maintenance of the differentiated state, and suggest potential human Staufen1 mRNA targets involved in this process.
authors.affiliation: Departamento de Biología Molecular y Celular, Centro Nacional de Biotecnología (CSIC), Madrid, Spain; Ciber de Enfermedades Respiratorias (ISCIII), Madrid, Spain.
authors.forename: Joan
authors.initials: J
authors.lastname: Peredo
authors.affiliation: Departamento de Biología Molecular y Celular, Centro Nacional de Biotecnología (CSIC), Madrid, Spain.
authors.forename: Patricia
authors.initials: P
authors.lastname: Villacé
authors.affiliation: Departamento de Biología Molecular y Celular, Centro Nacional de Biotecnología (CSIC), Madrid, Spain; Ciber de Enfermedades Respiratorias (ISCIII), Madrid, Spain.
authors.forename: Juan
authors.initials: J
authors.lastname: Ortín
authors.affiliation: Departamento de Biología Molecular y Celular, Centro Nacional de Biotecnología (CSIC), Madrid, Spain; Ciber de Enfermedades Respiratorias (ISCIII), Madrid, Spain.
authors.forename: Susana
authors.initials: S
authors.lastname: de Lucas
mesh_terms: D002454:Cell Differentiation; D002460:Cell Line; D003598:Cytoskeletal Proteins / Q000378:metabolism*; D003712:Dendrites / Q000502:physiology*; D006801:Humans; D035683:MicroRNAs / Q000378:metabolism*; D009474:Neurons / Q000166:cytology*; D011485:Protein Binding; D016601:RNA-Binding Proteins / Q000378:metabolism*
chemical_list: D003598:Cytoskeletal Proteins; D035683:MicroRNAs; D016601:RNA-Binding Proteins; C492185:STAU1 protein, human