title: IL-33 expression in the cerebral cortex following experimental subarachnoid hemorrhage in rats.
abstract: Subarachnoid hemorrhage (SAH) is a pervasive and devastating condition in which inflammatory and apoptotic pathways contribute to poor outcome. Interleukin-33 (IL-33) plays a crucial role in the inflammatory and apoptotic pathways through binding of the transmembrane ST2 receptor. This study investigated the expression and cellular localization of IL-33 in the cerebral cortex after SAH in order to clarify the role of IL-33 after SAH. Sprague-Dawley rats were randomly divided into sham and SAH groups and evaluated 2, 6, and 12 h and 1, 2, 3, and 5 days after the surgery, with SAH animals subjected to prechiasmatic cistern SAH. IL-33 expression was measured by western blot analysis, real-time PCR, immunohistochemistry, and immunofluorescence. The mRNA levels of tumor necrosis factor (TNF)-α and IL-1β were also assessed. The expression of IL-33, IL-1β, and TNF-α was markedly elevated in the SAH as compared to the sham group; IL-33 was mainly localized in neurons and astrocytes and not microglia after SAH. Moreover, a significant positive association was observed between IL-33 and IL-1β expression. These findings indicate that IL-33 might play an important role in the inflammatory response following SAH.
authors.affiliation: Department of Neurosurgery, School of Medicine, Southern Medical University (Guangzhou), Jinling Hospital, 305 East Zhongshan Road, Nanjing, 20002, Jiangsu Province, People's Republic of China.
authors.forename: Li-Tian
authors.initials: LT
authors.lastname: Huang
authors.forename: Hua
authors.initials: H
authors.lastname: Li
authors.forename: Qing
authors.initials: Q
authors.lastname: Sun
authors.forename: Ming
authors.initials: M
authors.lastname: Liu
authors.forename: Wei-De
authors.initials: WD
authors.lastname: Li
authors.forename: Song
authors.initials: S
authors.lastname: Li
authors.forename: Zhuang
authors.initials: Z
authors.lastname: Yu
authors.forename: Wu-Ting
authors.initials: WT
authors.lastname: Wei
authors.forename: Chun-Hua
authors.initials: CH
authors.lastname: Hang
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000378:metabolism; D002467:Cell Nucleus / Q000378:metabolism; D002540:Cerebral Cortex / Q000378:metabolism* / Q000473:pathology; D005455:Fluorescent Antibody Technique; D053583:Interleukin-1beta / Q000235:genetics / Q000378:metabolism; D000067596:Interleukin-33 / Q000235:genetics / Q000378:metabolism*; D008297:Male; D009474:Neurons / Q000378:metabolism; D017207:Rats, Sprague-Dawley; D060888:Real-Time Polymerase Chain Reaction; D013345:Subarachnoid Hemorrhage / Q000235:genetics / Q000378:metabolism*; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000378:metabolism; D015854:Up-Regulation
chemical_list: D053583:Interleukin-1beta; D000067596:Interleukin-33; D014409:Tumor Necrosis Factor-alpha