title: Epigenetic regulation of human hedgehog interacting protein in glioma cell lines and primary tumor samples.
abstract: Glioma constitutes one of the most common groups of brain tumors, and its prognosis is influenced by different genetic and epigenetic modulations. In this study, we demonstrated low or no expression of hedgehog interacting protein (HHIP) in most of the cell lines and primary glioma tumor samples. We further proceeded to promoter methylation study of this gene in the same cell lines and primary tumor samples and found 87 % (7/8) HHIP methylation in glioblastoma cell lines and 75 % (33/44) in primary tumor samples. These methylation pattern correlates with low or unexpressed HHIP in both cell lines and primary tumor samples. Our results suggest the possibility of epigenetic regulation of this gene in glioma, similarly to medulloblastoma, gastric, hepatic, and pancreatic cancers. Also, HHIP might be a diagnostic or prognostic marker in glioma and help to the detection of these tumors in early stages of disease.
authors.affiliation: Brain Tumor Biology Unit, University of Navarra School of Sciences, Pamplona, Spain.
authors.forename: Mehdi H
authors.initials: MH
authors.lastname: Shahi
authors.forename: Idoya
authors.initials: I
authors.lastname: Zazpe
authors.forename: Mohammad
authors.initials: M
authors.lastname: Afzal
authors.forename: Subrata
authors.initials: S
authors.lastname: Sinha
authors.forename: Robert B
authors.initials: RB
authors.lastname: Rebhun
authors.forename: Bárbara
authors.initials: B
authors.lastname: Meléndez
authors.forename: Juan A
authors.initials: JA
authors.lastname: Rey
authors.forename: Javier S
authors.initials: JS
authors.lastname: Castresana
mesh_terms: D000328:Adult; D001932:Brain Neoplasms / Q000235:genetics* / Q000473:pathology; D002352:Carrier Proteins / Q000096:biosynthesis* / Q000235:genetics; D045744:Cell Line, Tumor; D019175:DNA Methylation / Q000235:genetics*; D044127:Epigenesis, Genetic; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics; D005910:Glioma / Q000235:genetics* / Q000473:pathology; D006801:Humans; D008562:Membrane Glycoproteins / Q000096:biosynthesis* / Q000235:genetics; D011401:Promoter Regions, Genetic; D014157:Transcription Factors / Q000096:biosynthesis; D000071676:Zinc Finger Protein GLI1
chemical_list: D002352:Carrier Proteins; C400551:GLI1 protein, human; C117596:HHIP protein, human; D008562:Membrane Glycoproteins; D014157:Transcription Factors; D000071676:Zinc Finger Protein GLI1