title: Insect-specific production of new GameXPeptides in photorhabdus luminescens TTO1, widespread natural products in entomopathogenic bacteria.
abstract: Discovery of new natural products by heterologous expression reaches its limits, especially when specific building blocks are missing in the heterologous host or the production medium. Here, we describe the insect-specific production of the new GameXPeptides E-H (5-8) from Photorhabdus luminescens TTO1, which can be produced heterologously from expression of the GameXPeptide synthetase GxpS only upon supplementation of the production media with the missing building blocks, and thus must be regarded as the true natural products under natural conditions.
authors.affiliation: Merck Stiftungsprofessur für Molekulare Biotechnologie, Fachbereich der Biowissenschaften, Goethe Universität Frankfurt, Max-von-Laue-Strasse 9, 60438 Frankfurt am Main (Germany).
authors.forename: Friederike I
authors.initials: FI
authors.lastname: Nollmann
authors.forename: Christina
authors.initials: C
authors.lastname: Dauth
authors.forename: Geraldine
authors.initials: G
authors.lastname: Mulley
authors.forename: Carsten
authors.initials: C
authors.lastname: Kegler
authors.forename: Marcel
authors.initials: M
authors.lastname: Kaiser
authors.forename: Nick R
authors.initials: NR
authors.lastname: Waterfield
authors.forename: Helge B
authors.initials: HB
authors.lastname: Bode
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000378:metabolism*; D020869:Gene Expression Profiling; D007814:Larva / Q000382:microbiology; D009036:Moths / Q000382:microbiology*; D005810:Multigene Family; D009154:Mutation; D010453:Peptide Synthases / Q000235:genetics / Q000378:metabolism; D010455:Peptides / Q000737:chemistry* / Q000378:metabolism; D020637:Photorhabdus / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D015202:Protein Engineering / Q000379:methods; D064210:Secondary Metabolism
chemical_list: D001426:Bacterial Proteins; D010455:Peptides; D010453:Peptide Synthases
keywords: Photorhabdus; aminophenylalanine; environment-specific natural products; nonribosomal peptides