title: Application of thinned-skull cranial window to mouse cerebral blood flow imaging using optical microangiography.
abstract: In vivo imaging of mouse brain vasculature typically requires applying skull window opening techniques: open-skull cranial window or thinned-skull cranial window. We report non-invasive 3D in vivo cerebral blood flow imaging of C57/BL mouse by the use of ultra-high sensitive optical microangiography (UHS-OMAG) and Doppler optical microangiography (DOMAG) techniques to evaluate two cranial window types based on their procedures and ability to visualize surface pial vessel dynamics. Application of the thinned-skull technique is found to be effective in achieving high quality images for pial vessels for short-term imaging, and has advantages over the open-skull technique in available imaging area, surgical efficiency, and cerebral environment preservation. In summary, thinned-skull cranial window serves as a promising tool in studying hemodynamics in pial microvasculature using OMAG or other OCT blood flow imaging modalities.
authors.affiliation: Department of Bioengineering, University of Washington, Seattle, Washington, United States of America.
authors.forename: Yuandong
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Bioengineering, University of Washington, Seattle, Washington, United States of America; Department of Electrical Engineering, University of Washington, Seattle, Washington, United States of America.
authors.forename: Utku
authors.initials: U
authors.lastname: Baran
authors.affiliation: Department of Bioengineering, University of Washington, Seattle, Washington, United States of America.
authors.forename: Ruikang K
authors.initials: RK
authors.lastname: Wang
mesh_terms: D000818:Animals; D002533:Cerebral Angiography / Q000379:methods*; D002536:Cerebral Arteries / Q000000981:diagnostic imaging; D002550:Cerebral Veins / Q000000981:diagnostic imaging; D002560:Cerebrovascular Circulation; D051379:Mice; D008810:Mice, Inbred C57BL; D012886:Skull / Q000098:blood supply*