title: Genotypic analysis of Kaposi's sarcoma-associated herpesvirus from patients with Kaposi's sarcoma in Xinjiang, China.
abstract: Kaposi's sarcoma-associated herpesvirus (KSHV) is the causal agent of all forms of Kaposi's sarcoma (KS), including AIDS-KS, endemic KS, classic KS and iatrogenic KS. Based on Open reading frame (ORF) K1 sequence analysis, KSHV has been classified into seven major molecular subtypes (A, B, C, D, E, F and Z). The distribution of KSHV strains varies according to geography and ethnicity. Xinjiang is a unique region where the seroprevalence of KSHV is significantly higher than other parts of China. The genotyping of KSHV strains in this region has not been thoroughly studied. The present study aimed to evaluate the frequency of KSHV genotypes isolated from KS tissues in Classical KS and AIDS KS patients from Xinjiang, China. ORF-K1 of KSHV from tissue samples of 28 KS patients was amplified and sequenced. Two subtypes of KSHV were identified according to K1 genotyping. Twenty-three of them belonged to subtype A, while five of them were subtype C. More genotype A than genotype C strains were found in both Classical KS and AIDS KS. No significant difference was found in the prevalence of different genotype between Classical KS and AIDS KS.
authors.affiliation: State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China. annlooy@gmail.com.
authors.forename: Xinxing
authors.initials: X
authors.lastname: Ouyang
authors.affiliation: Laboratory of Xinjiang Endemic and Ethnic Diseases, Shihezi University, Shihezi 832002, China. yzeng910@163.com.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zeng
authors.affiliation: State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China. fubishi001@yeah.net.
authors.forename: Bishi
authors.initials: B
authors.lastname: Fu
authors.affiliation: Department of Microbiology, Anhui Medical University, Hefei 230032, China. Wangxiaowuxiaowu@sina.com.
authors.forename: Xiaowu
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Microbiology, Anhui Medical University, Hefei 230032, China. chenwei1413@163.com.
authors.forename: Wei
authors.initials: W
authors.lastname: Chen
authors.affiliation: Department of Microbiology, Anhui Medical University, Hefei 230032, China. yuanslience@163.com.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Fang
authors.affiliation: State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China.
authors.forename: Minhua
authors.initials: M
authors.lastname: Luo
authors.affiliation: Department of Microbiology, Anhui Medical University, Hefei 230032, China. wanglinding@ahmu.edu.cn.
authors.forename: Linding
authors.initials: L
authors.lastname: Wang
mesh_terms: D000328:Adult; D000368:Aged; D002681:China / Q000453:epidemiology / Q000208:ethnology; D005260:Female; D005838:Genotype; D019288:Herpesvirus 8, Human / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D008297:Male; D008875:Middle Aged; D016366:Open Reading Frames; D010802:Phylogeny; D012514:Sarcoma, Kaposi / Q000453:epidemiology / Q000208:ethnology / Q000821:virology*; D055815:Young Adult