title: Immunopotentiating nano-chitosan as potent vaccine carter for efficacious prophylaxis of filarial antigens.
abstract: Lymphatic filariasis (LF), a morbid vector-borne parasitic infection affects millions in tropical areas. Complete eradication can only be achieved by the development of a potent vaccine. Among the various filarial antigens that have been characterized, antigens Brugia malayi thioredoxin (TRX) and abundant larval transcript (ALT) have produced recognizable level of protection in Jirds, thereby evidenced to be good vaccine candidates. In this study an attempt was made to enhance their immunoprophylactic activity by encapsulating them in natural polysaccharide chitosan forming nanospheres (CN). High encapsulation efficiency for TRX (93%) in CN (TCN) and ALT-2 (90%) in CN (ACN) was achieved. Morphological studies confirmed the spherical and uniform distribution of nanospheres to be 220 nm. The electrostatic interaction between chitosan and the antigens were confirmed using differential scanning calorimetry and FT-IR. The study revealed the immunostimulatory property of chitosan providing enhanced level of proliferation for encapsulated antigens in peripheral blood mononuclear cells from endemic normal personals, at low concentration (TCN mean stimulation index (SI)=4.23±0.15 and ACN (SI)=4.05±0.33) compared to stimulation obtained by antigens alone. Hence, our study demonstrated that natural macromolecule derived CN can be used as efficacious immunostimulatory vaccine carter for LF thereby diminishing pathological sequel.
authors.affiliation: Anna University, Guindy, Chennai 600025, Tamil Nadu, India.
authors.forename: Balasubramaniyan
authors.initials: B
authors.lastname: Malathi
authors.affiliation: Centre for Biotechnology, Anna University, Guindy, Chennai 600025, Tamil Nadu, India.
authors.forename: Santhanam
authors.initials: S
authors.lastname: Mona
authors.affiliation: Anna University, Guindy, Chennai 600025, Tamil Nadu, India. Electronic address: tdevasenabio@annauniv.edu.
authors.forename: Devasena
authors.initials: D
authors.lastname: Thiyagarajan
authors.affiliation: Centre for Biotechnology, Anna University, Guindy, Chennai 600025, Tamil Nadu, India. Electronic address: kaliraj55@yahoo.co.in.
authors.forename: Perumal
authors.initials: P
authors.lastname: Kaliraj
mesh_terms: D000818:Animals; D000947:Antigens, Helminth / Q000276:immunology; D017178:Brugia malayi / Q000276:immunology; D048271:Chitosan / Q000737:chemistry* / Q000276:immunology; D004605:Elephantiasis, Filarial / Q000517:prevention & control; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000276:immunology; D008213:Lymphocyte Activation / Q000276:immunology; D054118:Nanospheres / Q000737:chemistry* / Q000648:ultrastructure; D017550:Spectroscopy, Fourier Transform Infrared; D013879:Thioredoxins / Q000008:administration & dosage / Q000276:immunology* / Q000302:isolation & purification; D014612:Vaccines / Q000737:chemistry* / Q000276:immunology
chemical_list: D000947:Antigens, Helminth; D014612:Vaccines; D013879:Thioredoxins; D048271:Chitosan
keywords: Abundant larval transcript-2; Chitosan nanospheres; Lymphatic filariasis; PBMC; Thioredoxin