title: Discovering human RNA aptamers by structure-based bioinformatics and genome-based in vitro selection.
abstract: In vitro selection and structure-based searches have emerged as useful techniques for the discoveries of structurally complex RNAs with high affinity and specificity toward metabolites. Here, we focus on the design of a human genomic library that serves as the DNA template for in vitro selection of RNA aptamers. In addition, the structural solutions obtained from the in vitro selection can be used for structure-based searches for discovery of analogous aptamers in various genomic databases.
authors.affiliation: Department of Pharmaceutical Sciences, University of California, Irvine, California, USA; Department of Molecular Biology and Biochemistry, University of California, Irvine, California, USA; Department of Chemistry, University of California, Irvine, California, USA.
authors.forename: Bao
authors.initials: B
authors.lastname: Ho
authors.affiliation: Department of Pharmaceutical Sciences, University of California, Irvine, California, USA; Department of Molecular Biology and Biochemistry, University of California, Irvine, California, USA; Department of Chemistry, University of California, Irvine, California, USA.
authors.forename: Julio
authors.initials: J
authors.lastname: Polanco
authors.affiliation: Department of Pharmaceutical Sciences, University of California, Irvine, California, USA; Department of Molecular Biology and Biochemistry, University of California, Irvine, California, USA; Department of Chemistry, University of California, Irvine, California, USA.
authors.forename: Randi
authors.initials: R
authors.lastname: Jimenez
authors.affiliation: Department of Pharmaceutical Sciences, University of California, Irvine, California, USA; Department of Molecular Biology and Biochemistry, University of California, Irvine, California, USA; Department of Chemistry, University of California, Irvine, California, USA. Electronic address: aluptak@uci.edu.
authors.forename: Andrej
authors.initials: A
authors.lastname: Lupták
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry* / Q000235:genetics*; D001483:Base Sequence; D019295:Computational Biology / Q000379:methods*; D015723:Gene Library; D015894:Genome, Human; D006801:Humans; D052156:SELEX Aptamer Technique / Q000379:methods*
chemical_list: D052157:Aptamers, Nucleotide
keywords: Bioinformatics search; Human-genomic library; In vitro selection; Pool design; RNA aptamers; SELEX; Structural based