title: Alanine mutants of the interface residues of human thymidylate synthase decode key features of the binding mode of allosteric anticancer peptides.
abstract: Allosteric peptide inhibitors of thymidylate synthase (hTS) bind to the dimer interface and stabilize the inactive form of the protein. Four interface residues were mutated to alanine, and interaction studies were employed to decode the key role of these residues in the peptide molecular recognition. This led to the identification of three crucial interface residues F59, L198, and Y202 that impart activity to the peptide inhibitors and suggest the binding area for further inhibitor design.
authors.affiliation: Department of Biochemistry and Biophysics, University of California-San Francisco , 600 16th Street, San Francisco, California 94158, United States.
authors.forename: Anna
authors.initials: A
authors.lastname: Tochowicz
authors.forename: Matteo
authors.initials: M
authors.lastname: Santucci
authors.forename: Puneet
authors.initials: P
authors.lastname: Saxena
authors.forename: Giambattista
authors.initials: G
authors.lastname: Guaitoli
authors.forename: Matteo
authors.initials: M
authors.lastname: Trande
authors.forename: Janet
authors.initials: J
authors.lastname: Finer-Moore
authors.forename: Robert M
authors.initials: RM
authors.lastname: Stroud
authors.forename: Maria P
authors.initials: MP
authors.lastname: Costi
mesh_terms: D000409:Alanine; D000494:Allosteric Regulation; D000970:Antineoplastic Agents / Q000138:chemical synthesis* / Q000494:pharmacology; D018360:Crystallography, X-Ray; D004791:Enzyme Inhibitors / Q000138:chemical synthesis* / Q000494:pharmacology; D006801:Humans; D016297:Mutagenesis, Site-Directed; D013329:Structure-Activity Relationship; D013940:Thymidylate Synthase / Q000037:antagonists & inhibitors* / Q000737:chemistry / Q000235:genetics
chemical_list: D000970:Antineoplastic Agents; D004791:Enzyme Inhibitors; D013940:Thymidylate Synthase; D000409:Alanine