title: Preparation of morusin from Ramulus mori and its effects on mice with transplanted H22 hepatocarcinoma.
abstract: In this study, we used branches Ramulus mori from cultivated mulberry Husang-32 (Morus multicaulis Perry) as the experimental material and anhydrous ethanol as the extraction solution to obtain the crude extract from the branch bark. The ethanolic extract was successively purified through a macroporous resin, Sephadex LH-20, and semipreparative reversed-phase high-performance liquid chromatography (RP-HPLC). The high-purity monomer was identified as morusin by HPLC with diode array detection (HPLC-DAD) and its UV spectrum. The contents of morusin exhibited almost no difference between the root and branch bark in Husang-32, and morusin was not detected in the leaves. Morusin is able to inhibit the tumor growth of transplanted H22 hepatocarcinoma in mice and has no side effects. The fluorescence quantitative real-time PCR (qRT-PCR) results indicate that morusin has a marked inhibitory effect on liver cancer cells through a mechanism that may be related increases in the expression of p53, Survivin, CyclinB1, and Caspase-3 and a decrease in NF-κ B gene expression. The influence of this compound is more apparent in the Caspase-3 and the NF-κ B genes.
authors.affiliation: Silk Biotechnology Laboratory, School of Biology and Basic Medical Sciences, Soochow University, Suzhou, 215123, People's Republic of China.
authors.forename: Liang-Ze
authors.initials: LZ
authors.lastname: Wan
authors.forename: Bin
authors.initials: B
authors.lastname: Ma
authors.forename: Yu-Qing
authors.initials: YQ
authors.lastname: Zhang
mesh_terms: D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000302:isolation & purification / Q000494:pharmacology*; D006528:Carcinoma, Hepatocellular / Q000188:drug therapy* / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D053148:Caspase 3 / Q000235:genetics / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects; D056744:Cyclin B1 / Q000819:agonists / Q000235:genetics / Q000378:metabolism; D000431:Ethanol; D005419:Flavonoids / Q000302:isolation & purification / Q000494:pharmacology*; D015972:Gene Expression Regulation, Neoplastic; D051034:Inhibitor of Apoptosis Proteins / Q000819:agonists / Q000235:genetics / Q000378:metabolism; D008114:Liver Neoplasms, Experimental / Q000188:drug therapy* / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D031625:Morus / Q000737:chemistry*; D016328:NF-kappa B / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D024301:Plant Bark / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry; D018517:Plant Roots / Q000737:chemistry; D012097:Repressor Proteins / Q000819:agonists / Q000235:genetics / Q000378:metabolism; D012997:Solvents; D000077022:Survivin; D047368:Tumor Burden / Q000187:drug effects; D016159:Tumor Suppressor Protein p53 / Q000819:agonists / Q000235:genetics / Q000378:metabolism
chemical_list: D000972:Antineoplastic Agents, Phytogenic; C117749:Birc5 protein, mouse; C534700:Ccnb1 protein, mouse; D056744:Cyclin B1; D005419:Flavonoids; D051034:Inhibitor of Apoptosis Proteins; D016328:NF-kappa B; D010936:Plant Extracts; D012097:Repressor Proteins; D012997:Solvents; D000077022:Survivin; D016159:Tumor Suppressor Protein p53; D000431:Ethanol; C057451:morusin; D053148:Caspase 3
keywords: HPLC-DAD; NF-κB; alcohol extract; morusin; mulberry