title: Pseudomonas granadensis sp. nov., a new bacterial species isolated from the Tejeda, Almijara and Alhama Natural Park, Granada, Spain.
abstract: During the course of screening bacterial isolates as sources of as-yet unknown bioactive compounds with pharmaceutical applications, a chemo-organotrophic, Gram-negative bacterium was isolated from a soil sample taken from the Tejeda, Almijara and Alhama Natural Park, Granada, Spain. Strain F-278,770(T) was oxidase- and catalase-positive, aerobic, with a respiratory type of metabolism with oxygen as the terminal electron acceptor, non-spore-forming and motile by one polar flagellum, although some cells had two polar flagella. Phylogenetic analysis of the 16S rRNA, gyrB, rpoB and rpoD genes revealed that strain F-278,770(T) belongs to the Pseudomonas koreensis subgroup (Pseudomonas fluorescens lineage), with Pseudomonas moraviensis, P. koreensis, P. baetica and P. helmanticensis as its closest relatives. Chemotaxonomic traits such as polar lipid and fatty acid compositions and G+C content of genomic DNA corroborated the placement of strain F-278,770(T) in the genus Pseudomonas. DNA-DNA hybridization assays and phenotypic traits confirmed that this strain represents a novel species of the genus Pseudomonas, for which the name Pseudomonas granadensis sp. nov. is proposed. The type strain is F-278,770(T) ( = DSM 28040(T) = LMG 27940(T)).
authors.affiliation: Fundación MEDINA, Avenida del Conocimiento, 3. Health Sciences Technology Park, 18016 Granada, Spain.
authors.forename: Javier
authors.initials: J
authors.lastname: Pascual
authors.affiliation: Fundación MEDINA, Avenida del Conocimiento, 3. Health Sciences Technology Park, 18016 Granada, Spain.
authors.forename: Marina
authors.initials: M
authors.lastname: García-López
authors.affiliation: Fundación MEDINA, Avenida del Conocimiento, 3. Health Sciences Technology Park, 18016 Granada, Spain.
authors.forename: Gerald F
authors.initials: GF
authors.lastname: Bills
authors.affiliation: Fundación MEDINA, Avenida del Conocimiento, 3. Health Sciences Technology Park, 18016 Granada, Spain.
authors.forename: Olga
authors.initials: O
authors.lastname: Genilloud
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D005407:Flagella; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D011549:Pseudomonas / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013030:Spain
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S