title: Development of an ionic liquid-based microwave-assisted method for the extraction and determination of taxifolin in different parts of Larix gmelinii.
abstract: An ionic liquid-based microwave-assisted extraction method (ILMAE) was successfully applied for the extraction of taxifolin from Larix gmelinii. Different kinds of 1-alkyl-3-methylimidazolium ionic liquids with different kinds of cations and anions were studied and 1-butyl-3-methylimidazolium bromide was chosen as the optimal solvent for taxifolin extraction. The optimal conditions of ILMAE were determined by single factor experiments and Box-Behnken design as follows: [C4mim]Br concentration of 1.00 M, soaking time of 2 h, liquid-solid ratio of 15:1 mL/g, microwave irradiation power of 406 W, microwave irradiation time of 14 min. No degradation of taxifolin had been observed under the optimum conditions as evidenced from the stability studies performed with standard taxifolin. Compared with traditional solvent and methods, ILMAE provided higher extraction yield, lower energy and time consumption. The distribution of taxifolin in different parts of larch and the influences of age, orientation, and season on the accumulation of taxifolin were analyzed for the sufficient utilization of L. gmelinii.
authors.affiliation: Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin 150040, China. zaizhiliu@hotmail.com.
authors.forename: Zaizhi
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Pharmacy & Medical Laboratory Department, Daqing Medical College, Daqing 163312, China. jiajiaplay2006@163.com.
authors.forename: Jia
authors.initials: J
authors.lastname: Jia
authors.affiliation: Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin 150040, China. chenfengli@163.com.
authors.forename: Fengli
authors.initials: F
authors.lastname: Chen
authors.affiliation: Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin 150040, China. yangfj@nefu.edu.cn.
authors.forename: Fengjian
authors.initials: F
authors.lastname: Yang
authors.affiliation: Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin 150040, China. zygorl@163.com.
authors.forename: Yuangang
authors.initials: Y
authors.lastname: Zu
authors.affiliation: Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin 150040, China. ylnefu@163.com.
authors.forename: Lei
authors.initials: L
authors.lastname: Yang
mesh_terms: D000838:Anions; D018533:Biomass; D002412:Cations; D002625:Chemistry, Organic / Q000379:methods*; D052578:Ionic Liquids / Q000737:chemistry*; D028221:Larix / Q000737:chemistry*; D008872:Microwaves; D018517:Plant Roots / Q000737:chemistry; D011794:Quercetin / Q000031:analogs & derivatives* / Q000737:chemistry / Q000302:isolation & purification; D012015:Reference Standards; D012044:Regression Analysis; D015203:Reproducibility of Results; D012621:Seasons; D012997:Solvents; D013997:Time Factors; D014934:Wood / Q000737:chemistry; D052584:Xylem / Q000737:chemistry
chemical_list: D000838:Anions; D002412:Cations; D052578:Ionic Liquids; D012997:Solvents; D011794:Quercetin; C003377:taxifolin