title: Murine model for non-invasive imaging to detect and monitor ovarian cancer recurrence.
abstract: Epithelial ovarian cancer is the most lethal gynecologic malignancy in the United States. Although patients initially respond to the current standard of care consisting of surgical debulking and combination chemotherapy consisting of platinum and taxane compounds, almost 90% of patients recur within a few years. In these patients the development of chemoresistant disease limits the efficacy of currently available chemotherapy agents and therefore contributes to the high mortality. To discover novel therapy options that can target recurrent disease, appropriate animal models that closely mimic the clinical profile of patients with recurrent ovarian cancer are required. The challenge in monitoring intra-peritoneal (i.p.) disease limits the use of i.p. models and thus most xenografts are established subcutaneously. We have developed a sensitive optical imaging platform that allows the detection and anatomical location of i.p. tumor mass. The platform includes the use of optical reporters that extend from the visible light range to near infrared, which in combination with 2-dimensional X-ray co-registration can provide anatomical location of molecular signals. Detection is significantly improved by the use of a rotation system that drives the animal to multiple angular positions for 360 degree imaging, allowing the identification of tumors that are not visible in single orientation. This platform provides a unique model to non-invasively monitor tumor growth and evaluate the efficacy of new therapies for the prevention or treatment of recurrent ovarian cancer.
authors.affiliation: Department of Obstetrics, Gynecology and Reproductive Sciences, Reproductive Immunology Unit, Yale University School of Medicine.
authors.forename: Natalia J
authors.initials: NJ
authors.lastname: Sumi
authors.affiliation: Department of Obstetrics, Gynecology and Reproductive Sciences, Reproductive Immunology Unit, Yale University School of Medicine.
authors.forename: Eydis
authors.initials: E
authors.lastname: Lima
authors.affiliation: NatureMost Laboratories.
authors.forename: John
authors.initials: J
authors.lastname: Pizzonia
authors.affiliation: Bruker Preclinical Imaging.
authors.forename: Sean P
authors.initials: SP
authors.lastname: Orton
authors.affiliation: Department of Obstetrics, Gynecology and Reproductive Sciences, Reproductive Immunology Unit, Yale University School of Medicine.
authors.forename: Vinicius
authors.initials: V
authors.lastname: Craveiro
authors.affiliation: Department of Obstetrics, Gynecology and Reproductive Sciences, Reproductive Immunology Unit, Yale University School of Medicine.
authors.forename: Wonduk
authors.initials: W
authors.lastname: Joo
authors.affiliation: Department of Obstetrics, Gynecology and Reproductive Sciences, Reproductive Immunology Unit, Yale University School of Medicine.
authors.forename: Jennie C
authors.initials: JC
authors.lastname: Holmberg
authors.affiliation: Department of Obstetrics, Gynecology and Reproductive Sciences, Reproductive Immunology Unit, Yale University School of Medicine.
authors.forename: Marta
authors.initials: M
authors.lastname: Gurrea
authors.affiliation: Department of Obstetrics, Gynecology and Reproductive Sciences, Reproductive Immunology Unit, Yale University School of Medicine.
authors.forename: Yang
authors.initials: Y
authors.lastname: Yang-Hartwich
authors.affiliation: Department of Obstetrics, Gynecology and Reproductive Sciences, Reproductive Immunology Unit, Yale University School of Medicine; ayesha.alvero@yale.edu.
authors.forename: Ayesha
authors.initials: A
authors.lastname: Alvero
authors.affiliation: Department of Obstetrics, Gynecology and Reproductive Sciences, Reproductive Immunology Unit, Yale University School of Medicine.
authors.forename: Gil
authors.initials: G
authors.lastname: Mor
mesh_terms: D000818:Animals; D000077216:Carcinoma, Ovarian Epithelial; D004195:Disease Models, Animal; D005260:Female; D064593:Heterografts; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D008991:Monitoring, Physiologic / Q000379:methods; D009364:Neoplasm Recurrence, Local / Q000175:diagnosis* / Q000188:drug therapy / Q000473:pathology; D009368:Neoplasm Transplantation; D009375:Neoplasms, Glandular and Epithelial / Q000175:diagnosis* / Q000188:drug therapy / Q000473:pathology; D061848:Optical Imaging / Q000379:methods*; D010051:Ovarian Neoplasms / Q000175:diagnosis* / Q000188:drug therapy / Q000473:pathology