title: LSM14A inhibits porcine reproductive and respiratory syndrome virus (PRRSV) replication by activating IFN-β signaling pathway in Marc-145.
abstract: Porcine reproductive and respiratory syndrome virus (PRRSV) is considered as a significant contributor to porcine reproductive and respiratory syndrome, one of the most economically important diseases for the pig industry worldwide. Emerging evidence indicates that pattern recognition receptors play key roles in recognizing pathogen-associated molecular patterns. In the present study, we investigated the effects of a novel pattern recognition receptor LSM14A in regulating PRRSV replication. Results in Marc-145 cells and porcine alveolar macrophages (PAMs) indicated that overexpression of porcine LSM14A effectively inhibited the replication of PRRSV, and knockdown of LSM14A by siRNA enhanced the replication of PRRSV. Mechanistically, LSM14A up-regulated the activities of IFN-β and ISRE promoters, enhanced the production of IFN-β, RIG-I, and ISGs, and inhibited the production of the inflammatory cytokines of TNF-α and IL-6 mRNA. Additionally, the expression pattern of LSM14A during the infection of PRRSV in Tongcheng and Large White pigs was suppressed by the PRRSV challenge. Taken together, our results suggest that LSM14A is an important PRR that inhibits PPRSV replication by inducing IFN-β signaling and restraining inflammatory responses. Furthermore, the down-regulation of LSM14A by PRRSV might represent an important mechanism by which PRRSV invades the host. Our study sheds light on the possibility of developing a new strategy to control this disease.
authors.affiliation: Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Ministry of Education and College of Animal Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China.
authors.forename: Zhenhong
authors.initials: Z
authors.lastname: Li
authors.forename: Rui
authors.initials: R
authors.lastname: Chen
authors.forename: Jinhua
authors.initials: J
authors.lastname: Zhao
authors.forename: Ziyu
authors.initials: Z
authors.lastname: Qi
authors.forename: Likai
authors.initials: L
authors.lastname: Ji
authors.forename: Yueran
authors.initials: Y
authors.lastname: Zhen
authors.forename: Bang
authors.initials: B
authors.lastname: Liu
mesh_terms: D000818:Animals; D002460:Cell Line; D002522:Chlorocebus aethiops; D055785:Gene Knockdown Techniques; D016899:Interferon-beta / Q000502:physiology*; D015850:Interleukin-6 / Q000235:genetics / Q000378:metabolism; D016676:Macrophages, Alveolar / Q000276:immunology / Q000378:metabolism / Q000821:virology; D019318:Porcine Reproductive and Respiratory Syndrome / Q000378:metabolism* / Q000821:virology; D019316:Porcine respiratory and reproductive syndrome virus / Q000502:physiology*; D011401:Promoter Regions, Genetic; D034741:RNA, Small Interfering / Q000235:genetics; D051192:Receptors, Pattern Recognition / Q000502:physiology*; D015398:Signal Transduction; D034421:Sus scrofa; D013552:Swine; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000378:metabolism; D014779:Virus Replication
chemical_list: D015850:Interleukin-6; D034741:RNA, Small Interfering; D051192:Receptors, Pattern Recognition; D014409:Tumor Necrosis Factor-alpha; D016899:Interferon-beta