title: Synergistic antiproliferative effects of methotrexate-loaded smart silica nanocomposites in MDA-MB-231 breast cancer cells.
abstract: In this study, the ability of methotrexate (MTX)-loaded stimuli-responsive novel silica nanocomposites (MSNs) (with mean diameter of ± 60 nm) in the induction of apoptosis, and change in the Bax/Bcl-2 mRNA levels, were investigated. MTT assay and RT -PCR analysis were performed on MDA-MB-231 breast cancer cells, to evaluate their anti-proliferative, apoptotic and anti-apoptotic effects. MTX-loaded MSNs caused marked decrease in the percentage of viable cells, with a significant down-regulation in the level of expression of the anti-apoptotic gene (Bcl-2), and up-regulation in the apoptotic gene (Bax). MTX-loaded MSNs increased the efficacy of the chemotherapeutic agents in the inhibition of cell proliferation and induction of apoptosis.
authors.affiliation: a Drug Applied Research Center, Tabriz University of Medical Sciences , Tabriz , Iran.|b Department of Biochemistry and Clinical Laboratories , Faculty of Medical Sciences, Tabriz University of Medical Sciences , Tabriz , Iran.
authors.forename: Neda
authors.initials: N
authors.lastname: Alidadiyani
authors.affiliation: a Drug Applied Research Center, Tabriz University of Medical Sciences , Tabriz , Iran.|c School of Advanced Medical Science, Tabriz University of Medical Sciences , Tabriz , Iran.|d Research Center for Pharmaceutical Nanotechnology, Tabriz University of Medical Sciences , Tabriz , Iran.
authors.forename: Roya
authors.initials: R
authors.lastname: Salehi
authors.affiliation: e Department of Molecular Medicine , Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences , Tabriz , Iran.
authors.forename: Shahrooz
authors.initials: S
authors.lastname: Ghaderi
authors.affiliation: a Drug Applied Research Center, Tabriz University of Medical Sciences , Tabriz , Iran.|b Department of Biochemistry and Clinical Laboratories , Faculty of Medical Sciences, Tabriz University of Medical Sciences , Tabriz , Iran.|c School of Advanced Medical Science, Tabriz University of Medical Sciences , Tabriz , Iran.|d Research Center for Pharmaceutical Nanotechnology, Tabriz University of Medical Sciences , Tabriz , Iran.
authors.forename: Nasser
authors.initials: N
authors.lastname: Samadi
authors.affiliation: a Drug Applied Research Center, Tabriz University of Medical Sciences , Tabriz , Iran.|c School of Advanced Medical Science, Tabriz University of Medical Sciences , Tabriz , Iran.
authors.forename: Soodabeh
authors.initials: S
authors.lastname: Davaran
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry* / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D015536:Down-Regulation / Q000187:drug effects; D004337:Drug Carriers / Q000737:chemistry* / Q000494:pharmacology; D065546:Drug Liberation; D019008:Drug Resistance, Neoplasm / Q000187:drug effects; D004357:Drug Synergism; D006801:Humans; D008727:Methotrexate / Q000737:chemistry* / Q000494:pharmacology*; D053761:Nanocomposites / Q000737:chemistry*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012822:Silicon Dioxide / Q000737:chemistry*; D051028:bcl-2-Associated X Protein / Q000235:genetics
chemical_list: D000970:Antineoplastic Agents; D004337:Drug Carriers; D019253:Proto-Oncogene Proteins c-bcl-2; D012333:RNA, Messenger; D051028:bcl-2-Associated X Protein; D012822:Silicon Dioxide; D008727:Methotrexate
keywords: Bak; Bax; Bcl-2; MDA-MB-231 breast cancer cell line; biocompatible polymer; drug delivery; mesoporous silica nanoparticles; methotrexate; nanomedicine