title: Association of interleukin-10 promoter region polymorphisms with risk factors of Atherosclerosis.
abstract: Atherosclerosis is considered as an inflammatory disease, and carotid artery intima-media thickness (IMT) and carotid plaque are generally used as intermediated phenotype of atherosclerosis. The aim of this study was to investigate whether carotid IMT and plaque are associated with promoter region polymorphisms of interleukin 10 (IL-10) gene. We recruited 135 subjects from a rural area of south-eastern part of South Korea. Three polymorphisms in the promoter region of IL-10 (-1082 A/G, -819 T/C and -592 A/C) were genotyped by pyrosequencing. Carotid IMT was measured at common carotid arteries, and carotid bulbs and cardiovascular risk factors such as cholesterol, blood pressure, uric acid and homocysteine were measured using blood samples. Subjects with the minor allele (C) of -819 T/C or the minor allele (C) of -592 A/C showed lower values in carotid IMT than those with major allele homozygote of each polymorphism (P = 0.018 and P = 0.031, respectively). Subjects with carotid plaque were significantly older and showed higher values in carotid IMT, uric acid and homocysteine than those without plaque (P < 0.01, respectively). In conclusion, the promoter region polymorphisms of IL-10 gene associate with carotid IMT and plaque. Further studies with larger samples are needed to provide stronger evidence to justify anti-atheromatous properties of IL-10.
authors.affiliation: Department of Preventive Medicine, School of Medicine, Keimyung University, Daegu, Korea.
authors.forename: G I
authors.initials: GI
authors.lastname: Yu
authors.forename: S-E
authors.initials: SE
authors.lastname: Jun
authors.forename: H-C
authors.initials: HC
authors.lastname: Cho
authors.forename: K O
authors.initials: KO
authors.lastname: Park
authors.forename: J-H
authors.initials: JH
authors.lastname: Chung
authors.forename: D H
authors.initials: DH
authors.lastname: Shin
authors.forename: I S
authors.initials: IS
authors.lastname: Chung
mesh_terms: D050197:Atherosclerosis / Q000453:epidemiology / Q000235:genetics*; D001794:Blood Pressure; D059168:Carotid Intima-Media Thickness; D002784:Cholesterol / Q000097:blood; D005260:Female; D006710:Homocysteine / Q000097:blood; D006801:Humans; D016753:Interleukin-10 / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D058226:Plaque, Atherosclerotic / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic / Q000235:genetics*; D012307:Risk Factors; D014527:Uric Acid / Q000097:blood
chemical_list: C508609:IL10 protein, human; D006710:Homocysteine; D016753:Interleukin-10; D014527:Uric Acid; D002784:Cholesterol