title: Engineered hyperphosphorylation of the β2-adrenoceptor prolongs arrestin-3 binding and induces arrestin internalization.
abstract: G protein-coupled receptor phosphorylation plays a major role in receptor desensitization and arrestin binding. It is, however, unclear how distinct receptor phosphorylation patterns may influence arrestin binding and subsequent trafficking. Here we engineer phosphorylation sites into the C-terminal tail of the β2-adrenoceptor (β2AR) and demonstrate that this mutant, termed β2AR(SSS), showed increased isoprenaline-stimulated phosphorylation and differences in arrestin-3 affinity and trafficking. By measuring arrestin-3 recruitment and the stability of arrestin-3 receptor complexes in real time using fluorescence resonance energy transfer and fluorescence recovery after photobleaching, we demonstrate that arrestin-3 dissociated quickly and almost completely from the β2AR, whereas the interaction with β2AR(SSS) was 2- to 4-fold prolonged. In contrast, arrestin-3 interaction with a β2-adrenoceptor fused to the carboxyl-terminal tail of the vasopressin type 2 receptor was nearly irreversible. Further analysis of arrestin-3 localization revealed that by engineering phosphorylation sites into the β2-adrenoceptor the receptor showed prolonged interaction with arrestin-3 and colocalization with arrestin in endosomes after internalization. This is in contrast to the wild-type receptor that interacts transiently with arrestin-3 at the plasma membrane. Furthermore, β2AR(SSS) internalized more efficiently than the wild-type receptor, whereas recycling was very similar for both receptors. Thus, we show how the interaction between arrestins and receptors can be increased with minimal receptor modification and that relatively modest increases in receptor-arrestin affinity are sufficient to alter arrestin trafficking.
authors.affiliation: Institut für Pharmakologie und Klinische Pharmazie, Philipps-Universität Marburg, Marburg, Germany (D.Z., M.B., C.K.); MRC Toxicology Unit, University of Leicester, Leicester, United Kingdom (A.J.B., A.B.T.); Department of Pharmacology and Toxicology, Kuwait University, Kuwait (S.A.-S.); and University of Applied Sciences Upper Austria, Wels, Austria (P.L., J.W.).
authors.forename: Diana
authors.initials: D
authors.lastname: Zindel
authors.affiliation: Institut für Pharmakologie und Klinische Pharmazie, Philipps-Universität Marburg, Marburg, Germany (D.Z., M.B., C.K.); MRC Toxicology Unit, University of Leicester, Leicester, United Kingdom (A.J.B., A.B.T.); Department of Pharmacology and Toxicology, Kuwait University, Kuwait (S.A.-S.); and University of Applied Sciences Upper Austria, Wels, Austria (P.L., J.W.).
authors.forename: Adrian J
authors.initials: AJ
authors.lastname: Butcher
authors.affiliation: Institut für Pharmakologie und Klinische Pharmazie, Philipps-Universität Marburg, Marburg, Germany (D.Z., M.B., C.K.); MRC Toxicology Unit, University of Leicester, Leicester, United Kingdom (A.J.B., A.B.T.); Department of Pharmacology and Toxicology, Kuwait University, Kuwait (S.A.-S.); and University of Applied Sciences Upper Austria, Wels, Austria (P.L., J.W.).
authors.forename: Suleiman
authors.initials: S
authors.lastname: Al-Sabah
authors.affiliation: Institut für Pharmakologie und Klinische Pharmazie, Philipps-Universität Marburg, Marburg, Germany (D.Z., M.B., C.K.); MRC Toxicology Unit, University of Leicester, Leicester, United Kingdom (A.J.B., A.B.T.); Department of Pharmacology and Toxicology, Kuwait University, Kuwait (S.A.-S.); and University of Applied Sciences Upper Austria, Wels, Austria (P.L., J.W.).
authors.forename: Peter
authors.initials: P
authors.lastname: Lanzerstorfer
authors.affiliation: Institut für Pharmakologie und Klinische Pharmazie, Philipps-Universität Marburg, Marburg, Germany (D.Z., M.B., C.K.); MRC Toxicology Unit, University of Leicester, Leicester, United Kingdom (A.J.B., A.B.T.); Department of Pharmacology and Toxicology, Kuwait University, Kuwait (S.A.-S.); and University of Applied Sciences Upper Austria, Wels, Austria (P.L., J.W.).
authors.forename: Julian
authors.initials: J
authors.lastname: Weghuber
authors.affiliation: Institut für Pharmakologie und Klinische Pharmazie, Philipps-Universität Marburg, Marburg, Germany (D.Z., M.B., C.K.); MRC Toxicology Unit, University of Leicester, Leicester, United Kingdom (A.J.B., A.B.T.); Department of Pharmacology and Toxicology, Kuwait University, Kuwait (S.A.-S.); and University of Applied Sciences Upper Austria, Wels, Austria (P.L., J.W.).
authors.forename: Andrew B
authors.initials: AB
authors.lastname: Tobin
authors.affiliation: Institut für Pharmakologie und Klinische Pharmazie, Philipps-Universität Marburg, Marburg, Germany (D.Z., M.B., C.K.); MRC Toxicology Unit, University of Leicester, Leicester, United Kingdom (A.J.B., A.B.T.); Department of Pharmacology and Toxicology, Kuwait University, Kuwait (S.A.-S.); and University of Applied Sciences Upper Austria, Wels, Austria (P.L., J.W.).
authors.forename: Moritz
authors.initials: M
authors.lastname: Bünemann
authors.affiliation: Institut für Pharmakologie und Klinische Pharmazie, Philipps-Universität Marburg, Marburg, Germany (D.Z., M.B., C.K.); MRC Toxicology Unit, University of Leicester, Leicester, United Kingdom (A.J.B., A.B.T.); Department of Pharmacology and Toxicology, Kuwait University, Kuwait (S.A.-S.); and University of Applied Sciences Upper Austria, Wels, Austria (P.L., J.W.) cornelius.krasel@staff.uni-marburg.de.
authors.forename: Cornelius
authors.initials: C
authors.lastname: Krasel
mesh_terms: D000595:Amino Acid Sequence; D019390:Arrestins / Q000235:genetics* / Q000378:metabolism*; D004705:Endocytosis / Q000502:physiology*; D057809:HEK293 Cells; D006801:Humans; D008969:Molecular Sequence Data; D010766:Phosphorylation / Q000502:physiology; D011485:Protein Binding / Q000502:physiology; D015202:Protein Engineering / Q000379:methods*; D021381:Protein Transport / Q000502:physiology; D018343:Receptors, Adrenergic, beta-2 / Q000235:genetics* / Q000378:metabolism*
chemical_list: C553443:ADRB2 protein, human; D019390:Arrestins; D018343:Receptors, Adrenergic, beta-2; C082333:arrestin3