title: MDR-1 gene C/T polymorphism in COPD: data from Aegean part of Turkey.
abstract: OBJECTIVE
  Genetic factors, in addition to oxidative stress factors, have been implicated in the development of chronic obstructive pulmonary disease (COPD). Multi-drug resistant-1 (MDR-1) is a gene located on chromosome 7 and the products of this gene protect lung tissue from oxidative stress. We searched the frequency of MDR-1 gene C/T polymorphism in patients with COPD and aimed to explain the association between MDR-1 gene and COPD development.
  
  
  METHODS
  47 patients with COPD and 64 healthy control participants were placed in this study. DNAs were extracted from blood samples and MDR-1 amplification of DNA was performed using polymerase chain reaction and enzyme digestion techniques.
  
  
  RESULTS
  The frequencies of MDR-1 genotypes were found 17.0% for CC, 51.1% for CT and 31.9% for TT in the COPD group and 39.1% for CC, 53.1% for CT and 7.8% for TT in the control group. The distribution of MDR-1 gene C alleles were found 32.3% in COPD group and 67.7% in control group; T alleles were found 55.1% in COPD group and 44.9% in control group. There was statistically significant difference between the groups for genotype and allele frequency of MDR-1 gene (P = 0.001).
  
  
  CONCLUSIONS
  TT genotype of MDR-1 gene was significantly more frequent in COPD patients. MDR-1 gene C/T polymorphism may play a role in COPD development.
authors.affiliation: Department of Chest Diseases, Faculty of Medicine, Dumlupınar University Kütahya 43100, Turkey.
authors.forename: Umran
authors.initials: U
authors.lastname: Toru
authors.affiliation: Department of Physiology, Faculty of Medicine, Dumlupınar University Kütahya 43100, Turkey.
authors.forename: Ceylan
authors.initials: C
authors.lastname: Ayada
authors.affiliation: Department of Physiology, Faculty of Medicine, Dumlupınar University Kütahya 43100, Turkey.
authors.forename: Osman
authors.initials: O
authors.lastname: Genç
authors.affiliation: Department of Physiology, Faculty of Medicine, Pamukkale University Denizli 20070, Turkey.
authors.forename: Sebahat
authors.initials: S
authors.lastname: Turgut
authors.affiliation: Department of Physiology, Faculty of Medicine, Pamukkale University Denizli 20070, Turkey.
authors.forename: Günfer
authors.initials: G
authors.lastname: Turgut
authors.affiliation: Department of Allergic Disease, Süreyyapaşa Chest Diseases and Thoracic Surgery Training and Research Hospital Istanbul 34844, Turkey.
authors.forename: Ismet
authors.initials: I
authors.lastname: Bulut
keywords: Multi-drug resistant-1 gene; chronic obstructive pulmonary disease; polymorphism