title: The effect of nimesulide on oxidative damage inflicted by ischemia-reperfusion on the rat renal tissue.
abstract: The objective of our study is to research biochemically and histopathologically the effect of nimesulide on oxidative damage inflicted by ischemia-reperfusion (I/R) on the rat renal tissue. Twenty-four albino Wistar type of male rats were used for the experiment. The animals were divided into groups as: renal ischemia-reperfusion control (RIR), nimesulide+renal ischemia-reperfusion of 50 mg/kg (NRIR-50), nimesulide+renal ischemia-reperfusion of 100 mg/kg (NRIR-100), and sham groups (SG). In NRIR-50 and NRIR-100 groups were given nimesulide, and RIR and SG groups were given distilled water, an hour after anesthesia. Groups, except for the SG group, 1-h-ischemia and then 6-h-reperfusion were performed. In the renal tissue of the RIR group in which the malondialdehyde (MDA), myeloperoxidase (MPO), and 8-hydroxyguanine (8-OHGua) levels were measured, the COX-1 and COX-2 activities were recorded. Nimesulide at 100 mg/kg doses reduced the oxidant parameters more significantly than 50 mg/kg doses; on the other hand, it raised the antioxidant parameters. It has been shown that 100 mg/kg doses of nimesulide prevented the renal I/R damage more significantly than a dose of 50 mg/kg, which shows that nimesulide, in clinics, could be used in the prevention of I/R damage.
authors.affiliation: Department of Nursing, School of Health Services, Recep Tayyip Erdogan University , Rize , Turkey .
authors.forename: Zeynep
authors.initials: Z
authors.lastname: Suleyman
authors.forename: Ebru
authors.initials: E
authors.lastname: Sener
authors.forename: Nezahat
authors.initials: N
authors.lastname: Kurt
authors.forename: Mehmet
authors.initials: M
authors.lastname: Comez
authors.forename: Turgut
authors.initials: T
authors.lastname: Yapanoglu
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D007668:Kidney / Q000473:pathology; D007674:Kidney Diseases / Q000188:drug therapy / Q000209:etiology / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D009195:Peroxidase / Q000378:metabolism; D010975:Platelet Aggregation Inhibitors / Q000494:pharmacology; D011451:Prostaglandin-Endoperoxide Synthases / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000188:drug therapy / Q000378:metabolism; D013449:Sulfonamides / Q000494:pharmacology*; D013997:Time Factors; D016896:Treatment Outcome
chemical_list: D000975:Antioxidants; D010975:Platelet Aggregation Inhibitors; D013449:Sulfonamides; D008315:Malondialdehyde; D009195:Peroxidase; D011451:Prostaglandin-Endoperoxide Synthases; C012655:nimesulide
keywords: Ischemia; nimesulide; rat; renal; reperfusion