title: [Diagnostic value of urine HBV DNA for hepatitis B virus-associated glomerulonephritis].
abstract: OBJECTIVE
  To investigate the potential value of urine hepatitis B virus (HBV) DNA as a new noninvasive diagnostic indicator for HBV-associated glomerulonephritis (HBV-GN).
  
  
  METHODS
  A total of 152 patients including 66 with HBV-GN, 66 with non-HBV-GN, and 20 with chronic hepatitis B (CHB) without renal disease were examined for serum and urine HBV DNA levels using polymerase chain reaction (PCR) and for 5 serum HBV markers using enzyme-linked immunosorbent assays.
  
  
  RESULTS
  Twenty-two patients (33%) in the HBV-GN group, but none in the other two groups, were found positive for urine HBV DNA. In the diagnosis of HBV-GN, urine HBV DNA had a high specificity (0.98), a good positive predictive value (PPV, 0.96), and a modest negative predictive value (NPV, 0.60). Urine HBV DNA, alone or in combination with serum HBeAg, was superior in the diagnosis of HBV-GN to the combination of urine HBV DNA with serum HBV DNA, hepatitis B surface antigen and the hepatitis B e antigen.
  
  
  CONCLUSIONS
  Urine HBV DNA may be one of the new noninvasive diagnostic criterion for HBV-GN.
authors.affiliation: Department of Nephrology, Guangdong General Hospital/Guangdong Academy of Medical Sciences, Guangzhou 510080, China. E-mail: 15010942109@139.com.
authors.forename: Lifen
authors.initials: L
authors.lastname: Wang
authors.forename: Zhiming
authors.initials: Z
authors.lastname: Ye
authors.forename: Lixia
authors.initials: L
authors.lastname: Xu
authors.forename: Bin
authors.initials: B
authors.lastname: Zhang
authors.forename: Huaban
authors.initials: H
authors.lastname: Liang
authors.forename: Zhonglin
authors.initials: Z
authors.lastname: Feng
authors.forename: Shuangxin
authors.initials: S
authors.lastname: Liu
authors.forename: Wei
authors.initials: W
authors.lastname: Shi
mesh_terms: D015415:Biomarkers / Q000097:blood / Q000652:urine*; D004279:DNA, Viral / Q000097:blood / Q000652:urine*; D004797:Enzyme-Linked Immunosorbent Assay; D005921:Glomerulonephritis / Q000175:diagnosis* / Q000821:virology; D006514:Hepatitis B Surface Antigens / Q000097:blood; D006513:Hepatitis B e Antigens / Q000097:blood; D019694:Hepatitis B, Chronic / Q000150:complications*; D006801:Humans; D016133:Polymerase Chain Reaction; D011237:Predictive Value of Tests; D012680:Sensitivity and Specificity
chemical_list: D015415:Biomarkers; D004279:DNA, Viral; D006514:Hepatitis B Surface Antigens; D006513:Hepatitis B e Antigens