title: GhDRIN1, a novel drought-induced gene of upland cotton (Gossypium hirsutum L.) confers abiotic and biotic stress tolerance in transgenic tobacco.
abstract: A novel stress tolerance cDNA fragment encoding GhDRIN1 protein was identified and its regulation was studied in cotton boll tissues and seedlings subjected to various biotic and abiotic stresses. Phylogenetic and conserved domain prediction indicated that GhDRIN1 was annotated with a hypothetical protein of unknown function. Subcellular localization showed that GhDRIN1 is localized in the chloroplasts. The promoter sequence was isolated and subjected to in silico study. Various cis-acting elements responsive to biotic and abiotic stresses and hormones were found. Transgenic tobacco seedlings exhibited better growth on amended MS medium and showed minimal leaf damage in insect bioassays carried out with Helicoverpa armigera larvae. Transgenic tobacco showed better tolerance to water-deficit and fast recovered upon rewatering. Present work demonstrated that GhDRIN1, a novel stress tolerance gene of cotton, positively regulates the response to biotic and abiotic stresses in transgenic tobacco.
authors.affiliation: National Research Centre on Plant Biotechnology, LBS Building, Pusa Campus, New Delhi, 110012, India.
authors.forename: Gurusamy
authors.initials: G
authors.lastname: Dhandapani
authors.forename: Azhagiyamanavalan
authors.initials: A
authors.lastname: Lakshmi Prabha
authors.forename: Mogilicherla
authors.initials: M
authors.lastname: Kanakachari
authors.forename: Mullapudi Lakshmi Venkata
authors.initials: ML
authors.lastname: Phanindra
authors.forename: Narayanasamy
authors.initials: N
authors.lastname: Prabhakaran
authors.forename: Sellamuthu
authors.initials: S
authors.lastname: Gothandapani
authors.forename: Kethireddy Venkata
authors.initials: KV
authors.lastname: Padmalatha
authors.forename: Amolkumar U
authors.initials: AU
authors.lastname: Solanke
authors.forename: Polumetla Ananda
authors.initials: PA
authors.lastname: Kumar
mesh_terms: D000818:Animals; D060365:Chloroplast Proteins / Q000235:genetics / Q000378:metabolism; D003470:Culture Media / Q000737:chemistry; D003681:Dehydration; D015870:Gene Expression; D003368:Gossypium / Q000235:genetics*; D007915:Lepidoptera / Q000502:physiology; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D030821:Plants, Genetically Modified; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D013312:Stress, Physiological; D014026:Nicotiana / Q000235:genetics / Q000469:parasitology / Q000502:physiology*
chemical_list: D060365:Chloroplast Proteins; D003470:Culture Media; D010940:Plant Proteins; D011994:Recombinant Proteins