title: Differential Expression of Antioxidant Enzymes During Degradation of Azo Dye Reactive black 8 in Hairy roots of Physalis minima L.
abstract: The enzymes involved in the protection of plant metabolism in presence of azo dye was characterized by studying activities of the role of antioxidant enzymes in the hairy roots (HRs) of Physalis minima L. during degradation of an azo dye, Reactive Black 8 (RB8). When the HRs were exposed to RB8 (30 mg L(-1)), a  nine fold increase in SOD activity was observed after 24 h, while 22 and 50 fold increase in activity was observed for POX and APX respectively after 72 h, whereas there was no significant change in activity of CAT. The activation of different antioxidant enzymes at different time intervals under dye stress suggests the synchronized functioning of antioxidant machinery to protect the HRs from oxidative damage. FTIR analysis confirmed the degradation of dye and the non-toxic nature of metabolites formed after dye degradation was confirmed by phytotoxicity study.
authors.affiliation: a Department of Biotechnology and Bioinformatics , Padmashree Dr. D.Y. Patil University , Sector 15/50, CBD, Belapur, Navi Mumbai (M.S) , India.
authors.forename: Pamela
authors.initials: P
authors.lastname: Jha
authors.forename: Nikita
authors.initials: N
authors.lastname: Modi
authors.forename: Renitta
authors.initials: R
authors.lastname: Jobby
authors.forename: Neetin
authors.initials: N
authors.lastname: Desai
mesh_terms: D000975:Antioxidants / Q000378:metabolism*; D001391:Azo Compounds / Q000378:metabolism*; D001673:Biodegradation, Environmental; D004396:Coloring Agents / Q000378:metabolism*; D018384:Oxidative Stress; D032321:Physalis / Q000737:chemistry / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*; D018517:Plant Roots / Q000737:chemistry / Q000201:enzymology / Q000235:genetics / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D013482:Superoxide Dismutase / Q000235:genetics / Q000378:metabolism
chemical_list: D000975:Antioxidants; D001391:Azo Compounds; D004396:Coloring Agents; D017382:Reactive Oxygen Species; D013482:Superoxide Dismutase
keywords: APX; Agrobacterium rhizogenes; CAT; POX; ROS; SOD