title: Influence of processing procedure on the quality of Radix Scrophulariae: a quantitative evaluation of the main compounds obtained by accelerated solvent extraction and high-performance liquid chromatography.
abstract: An improved high-performance liquid chromatography with diode array detection combined with accelerated solvent extraction method was used to simultaneously determine six compounds in crude and processed Radix Scrophulariae samples. Accelerated solvent extraction parameters such as extraction solvent, temperature, number of cycles, and analysis procedure were systematically optimized. The results indicated that compared with crude Radix Scrophulariae samples, the processed samples had lower contents of harpagide and harpagoside but higher contents of catalpol, acteoside, angoroside C, and cinnamic acid. The established method was sufficiently rapid and reliable for the global quality evaluation of crude and processed herbal medicines.
authors.affiliation: College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, P. R. China; Research Center of TCM Processing Technology, Zhejiang Chinese Medical University, Hangzhou, P. R. China.
authors.forename: Gang
authors.initials: G
authors.lastname: Cao
authors.forename: Xin
authors.initials: X
authors.lastname: Wu
authors.forename: Qinglin
authors.initials: Q
authors.lastname: Li
authors.forename: Hao
authors.initials: H
authors.lastname: Cai
authors.forename: Baochang
authors.initials: B
authors.lastname: Cai
authors.forename: Xuemei
authors.initials: X
authors.lastname: Zhu
mesh_terms: D002851:Chromatography, High Pressure Liquid; D002934:Cinnamates / Q000032:analysis / Q000302:isolation & purification; D003373:Coumaric Acids / Q000032:analysis / Q000302:isolation & purification; D005960:Glucosides / Q000032:analysis / Q000302:isolation & purification; D006027:Glycosides / Q000032:analysis / Q000302:isolation & purification; D057888:Iridoid Glucosides / Q000032:analysis / Q000302:isolation & purification; D057889:Iridoid Glycosides / Q000032:analysis / Q000302:isolation & purification; D010636:Phenols / Q000032:analysis / Q000302:isolation & purification; D011714:Pyrans / Q000032:analysis / Q000302:isolation & purification; D011786:Quality Control; D032266:Scrophularia / Q000737:chemistry*; D012997:Solvents / Q000737:chemistry; D014312:Trisaccharides / Q000032:analysis / Q000302:isolation & purification; D059808:Polyphenols
chemical_list: D002934:Cinnamates; D003373:Coumaric Acids; D005960:Glucosides; D006027:Glycosides; D057888:Iridoid Glucosides; D057889:Iridoid Glycosides; D010636:Phenols; D011714:Pyrans; D012997:Solvents; D014312:Trisaccharides; C413578:angoroside C; C029010:cinnamic acid; C078040:catalpol; C058956:acteoside; D059808:Polyphenols; C033249:harpagoside; C033250:harpagide
keywords: Accelerated solvent extraction; High-performance liquid chromatography; Processing procedures; Quantitative evaluation; Radix Scrophulariae