title: Impact of monitoring blood volume in the BD BACTEC™ FX blood culture system: virtual volume versus actual volume.
abstract: The BACTEC™ FX (BD, USA) has been recently upgraded to measure virtual blood volume (VV) in batches of 25 PLUS Aerobic/F bottles. VVs were compared with manually measured actual volumes (AVs). The mean AV was larger in anaerobic bottles: 2.6±1.9mL in 2995 Aerobic/F bottles and 3.3±1.8mL in 3053 Lytic/10 Anaerobic/F bottles (P<0.001). The VV of Aerobic/F bottles was 2.4±1.4mL, and there was a significant correlation between the VVs and AVs of each department (P<0.001). The potential positivity in aerobic bottles increased 1.2-fold with a 1-mL increase in the AV (P<0.001). AV and VV were consistently less than adequate. Although the proportional effect of volume on positivity can be proven only with the AV, VVs are a reliable substitute for AVs to monitor the overall blood volumes and provide feedback to the individual departments or locations.
authors.affiliation: Department of Laboratory Medicine, University of Ulsan College of Medicine and Asan Medical Center, Seoul, Republic of Korea.
authors.forename: Jeonghyun
authors.initials: J
authors.lastname: Chang
authors.affiliation: Department of Laboratory Medicine, University of Ulsan College of Medicine and Asan Medical Center, Seoul, Republic of Korea.
authors.forename: Jeong Su
authors.initials: JS
authors.lastname: Park
authors.affiliation: Department of Laboratory Medicine, University of Ulsan College of Medicine and Asan Medical Center, Seoul, Republic of Korea.
authors.forename: Sookja
authors.initials: S
authors.lastname: Park
authors.affiliation: Department of Laboratory Medicine, University of Ulsan College of Medicine and Asan Medical Center, Seoul, Republic of Korea.
authors.forename: Byeonghoo
authors.initials: B
authors.lastname: Choi
authors.affiliation: Department of Laboratory Medicine, University of Ulsan College of Medicine and Asan Medical Center, Seoul, Republic of Korea.
authors.forename: Nam Seop
authors.initials: NS
authors.lastname: Yoon
authors.affiliation: Department of Laboratory Medicine, University of Ulsan College of Medicine and Asan Medical Center, Seoul, Republic of Korea.
authors.forename: Heungsup
authors.initials: H
authors.lastname: Sung
authors.affiliation: Department of Laboratory Medicine, University of Ulsan College of Medicine and Asan Medical Center, Seoul, Republic of Korea. Electronic address: mnkim@amc.seoul.kr.
authors.forename: Mi-Na
authors.initials: MN
authors.lastname: Kim
mesh_terms: D000328:Adult; D016470:Bacteremia / Q000175:diagnosis*; D001431:Bacteriological Techniques / Q000379:methods*; D001769:Blood / Q000382:microbiology*; D006801:Humans; D011446:Prospective Studies; D012680:Sensitivity and Specificity; D013048:Specimen Handling / Q000379:methods*; D014481:United States
keywords: Aerobic bottle; Anaerobic bottle; Blood culture; Blood volume; Monitoring