title: [Improving thermal stability of xylanase by introducing aromatic residues at the N-terminus].
abstract: Thermophilic and alkalophilic xylanases have great potential in the pulp bleaching industry. In order to improve the thermal stability of an alkaline family 11 xylanase Xyn11A-LC, aromatic residues were introduced into the N-terminus of the enzyme by rational design. The mutant increased the optimum temperature by 5 degrees C. The wild type had a half-time of 22 min at 65 degrees C and pH 8.0 (Tris-HCl buffer). Under the same condition, the mutant had the half-time of 106 min. CD spectroscopy revealed that the melting temperature (T(m)) values of the wild type and mutant were 55.3 degrees C and 67.9 degrees C, respectively. These results showed that the introduction of aromatic residues could enhance the thermal stability of Xyn11A-LC.
authors.forename: Wenqin
authors.initials: W
authors.lastname: Bai
authors.forename: Luhong
authors.initials: L
authors.lastname: Yang
authors.forename: Yanhe
authors.initials: Y
authors.lastname: Ma
mesh_terms: D043364:Endo-1,4-beta Xylanases / Q000737:chemistry*; D004795:Enzyme Stability; D006863:Hydrogen-Ion Concentration; D015202:Protein Engineering; D013696:Temperature
chemical_list: D043364:Endo-1,4-beta Xylanases