title: A new GH43 α-arabinofuranosidase from Humicola insolens Y1: biochemical characterization and synergistic action with a xylanase on xylan degradation.
abstract: A new α-arabinofuranosidase gene (Hiabf43) was cloned from Humicola insolens Y1 and successfully expressed in Pichia pastoris GS115. Deduced HiAbf43 contained a putative signal peptide and a catalytic domain of glycoside hydrolase (GH) family 43. Purified recombinant HiAbf43 showed optimal activity at pH 5.0 and 50 °C, and was active over a broad pH range. The enzyme was specific for the cleavage of α-1,3-linkage and showed high activity against 4-nitrophenyl α-L-arabinofuranoside, debranched arabinan, and sugar beet arabinan. Sequential addition of HiAbf43 followed by Xyn11A increased the degradation efficiency of birchwood and beechwood xylans but not wheat arabinoxylan. The synergy degree was high up to 1.21-fold.
authors.affiliation: Key Laboratory for Feed Biotechnology of the Ministry of Agriculture, Feed Research Institute, Chinese Academy of Agricultural Sciences, No. 12 Zhongguancun South Street, Beijing, 100081, People's Republic of China.
authors.forename: Xinzhuo
authors.initials: X
authors.lastname: Yang
authors.forename: Pengjun
authors.initials: P
authors.lastname: Shi
authors.forename: Rui
authors.initials: R
authors.lastname: Ma
authors.forename: Huiying
authors.initials: H
authors.lastname: Luo
authors.forename: Huoqing
authors.initials: H
authors.lastname: Huang
authors.forename: Peilong
authors.initials: P
authors.lastname: Yang
authors.forename: Bin
authors.initials: B
authors.lastname: Yao
mesh_terms: D000595:Amino Acid Sequence; D001089:Arabinose / Q000031:analogs & derivatives / Q000378:metabolism; D020134:Catalytic Domain; D003001:Cloning, Molecular; D004357:Drug Synergism; D043364:Endo-1,4-beta Xylanases / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism* / Q000494:pharmacology; D057075:Enzyme Assays; D005656:Fungal Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism* / Q000494:pharmacology; D015870:Gene Expression; D006026:Glycoside Hydrolases / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism* / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010843:Pichia / Q000201:enzymology / Q000235:genetics; D011134:Polysaccharides / Q000378:metabolism; D021382:Protein Sorting Signals; D011994:Recombinant Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D004718:Saccharomycetales / Q000737:chemistry* / Q000201:enzymology; D016415:Sequence Alignment; D013379:Substrate Specificity; D013696:Temperature; D014934:Wood / Q000187:drug effects; D014990:Xylans / Q000378:metabolism*
chemical_list: D005656:Fungal Proteins; D011134:Polysaccharides; D021382:Protein Sorting Signals; D011994:Recombinant Proteins; D014990:Xylans; C030080:araban; C017567:4-nitrophenyl-alpha-L-arabinofuranoside; D001089:Arabinose; D006026:Glycoside Hydrolases; C019713:alpha-N-arabinofuranosidase; D043364:Endo-1,4-beta Xylanases