title: Metabolic engineering of strains: from industrial-scale to lab-scale chemical production.
abstract: A plethora of successful metabolic engineering case studies have been published over the past several decades. Here, we highlight a collection of microbially produced chemicals using a historical framework, starting with titers ranging from industrial scale (more than 50 g/L), to medium-scale (5-50 g/L), and lab-scale (0-5 g/L). Although engineered Escherichia coli and Saccharomyces cerevisiae emerge as prominent hosts in the literature as a result of well-developed genetic engineering tools, several novel native-producing strains are gaining attention. This review catalogs the current progress of metabolic engineering towards production of compounds such as acids, alcohols, amino acids, natural organic compounds, and others.
authors.affiliation: McKetta Department of Chemical Engineering, The University of Texas at Austin, 200 E Dean Keeton St. Stop C0400, Austin, TX, 78712, USA.
authors.forename: Jie
authors.initials: J
authors.lastname: Sun
authors.forename: Hal S
authors.initials: HS
authors.lastname: Alper
mesh_terms: D000143:Acids / Q000378:metabolism; D000438:Alcohols / Q000378:metabolism; D000596:Amino Acids / Q000096:biosynthesis / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D007218:Industrial Microbiology / Q000379:methods*; D007753:Laboratories; D060847:Metabolic Engineering / Q000379:methods*; D009930:Organic Chemicals / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism
chemical_list: D000143:Acids; D000438:Alcohols; D000596:Amino Acids; D009930:Organic Chemicals