title: Effects of La(III) and Ca(II) on isolated Carassius auratus liver mitochondria: heat production and mitochondrial permeability transition.
abstract: The effects of lanthanum and calcium on heat production of mitochondria isolated from Carassius auratus liver were investigated by microcalorimetry, and their effects on mitochondrial swelling and membrane potential (Δψ) were determined by spectroscopic methods. La(3+) showed only inhibitory action on mitochondrial energy turnover with inhibition concentration of 50 % (IC50) being 71.2 μmol L(-1). Similarly, Ca(2+) restrained the heat production of mitochondria, and the IC50 of Ca(2+) was much higher than that of La(3+). In the spectroscopic experiments, La(3+) and Ca(2+) induced fish liver mitochondrial swelling and decreased membrane potential (Δψ), and the induction ability of La(3+) was stronger than that of Ca(2+). It is concluded that the effects of La(3+) and Ca(2+) on fish liver mitochondria differ, and La represents toxic action rather than Ca analogy.
authors.affiliation: College of Chemistry and Environmental Engineering, Yangtze University, Jingzhou, Hubei, 434023, People's Republic of China.
authors.forename: Man
authors.initials: M
authors.lastname: Wu
authors.forename: Jia-Ling
authors.initials: JL
authors.lastname: Gao
authors.forename: Mei-Xiang
authors.initials: MX
authors.lastname: Sun
authors.forename: Ye-Zhong
authors.initials: YZ
authors.lastname: Zhang
authors.forename: Yi
authors.initials: Y
authors.lastname: Liu
authors.forename: Jie
authors.initials: J
authors.lastname: Dai
mesh_terms: D000818:Animals; D002118:Calcium / Q000494:pharmacology*; D006054:Goldfish / Q000378:metabolism*; D007811:Lanthanum / Q000494:pharmacology*; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects*; D008930:Mitochondria, Liver / Q000378:metabolism*; D051336:Mitochondrial Membranes / Q000378:metabolism*; D010539:Permeability
chemical_list: D007811:Lanthanum; D002118:Calcium