title: Miniaturized paper-based gene sensor for rapid and sensitive identification of contagious plant virus.
abstract: Plant viruses cause significant production and economic losses in the agricultural industry worldwide. Rapid and early identification of contagious plant viruses is an essential prerequisite for the effective control of further spreading of infection. In this work, we describe a miniaturized paper-based gene sensor for the rapid and sensitive identification of a contagious plant virus. Our approach makes use of hybridization-mediated target capture based on a miniaturized lateral flow platform and gold nanoparticle colorimetric probes. The captured colorimetric probes on the test line and control line of the gene sensor produce characteristic red bands, enabling visual detection of the amplified products within minutes without the need for sophisticated instruments or the multiple incubation and washing steps performed in most other assays. Quantitative analysis is realized by recording the optical intensity of the test line. The sensor was used successfully for the identification of banana bunchy top virus (BBTV). The detection limit was 0.13 aM of gene segment, which is 10 times higher than that of electrophoresis and provides confirmation of the amplified products. We believe that this simple, rapid, and sensitive bioactive platform has great promise for warning against plant diseases in agricultural production.
authors.affiliation: MOE Key Laboratory of Laser Life Science & Institute of Laser Life Science, College of Biophotonics, South China Normal University , Guangzhou 510631, China.
authors.forename: Jitao
authors.initials: J
authors.lastname: Wei
authors.forename: Hongxing
authors.initials: H
authors.lastname: Liu
authors.forename: Fang
authors.initials: F
authors.lastname: Liu
authors.forename: Minjun
authors.initials: M
authors.lastname: Zhu
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Zhou
authors.forename: Da
authors.initials: D
authors.lastname: Xing
mesh_terms: D052658:Babuvirus / Q000235:genetics* / Q000302:isolation & purification*; D015374:Biosensing Techniques / Q000295:instrumentation; D003124:Colorimetry / Q000295:instrumentation; D018744:DNA, Plant / Q000032:analysis* / Q000235:genetics; D017403:In Situ Hybridization / Q000295:instrumentation; D008904:Miniaturization; D028521:Musa / Q000821:virology*; D020411:Oligonucleotide Array Sequence Analysis / Q000295:instrumentation*; D010209:Paper
chemical_list: D018744:DNA, Plant
keywords: contagious plant virus; gold nanoparticle colorimetric probes; low-cost diagnostics; paper-based gene sensor; visual detection