title: Prognostic role of SIRT1 in hepatocellular carcinoma.
abstract: OBJECTIVE
  To determine the clinical significance of silent mating type information regulation 2 homolog 1 (SIRT1) expression in Hepatocellular Carcinoma (HCC) and its association with P53 and Yes-associated protein 2 (YAP2) expression.
  
  
  METHODS
  Observational study.
  
  
  METHODS
  Department of General Surgery, China-Japan Union Hospital of Jilin University, Changchun, China, from January 2000 to January 2010.
  
  
  METHODS
  Tissue microarray technique and immunohistochemistry were conducted to detect the expression of SIRT1, P53 and YAP2 proteins in 300 self-paired HCC samples. Associations with clinicopathologic manifestations were analyzed, overall survival analysis and multivariate analysis were performed.
  
  
  RESULTS
  By tissue microarray technique and immunohistochemistry on 300 self-paired HCC samples, it was found that SIRT1, P53 and YAP2 were significantly overexpressed in HCC tumor tissues compared with adjacent non-tumor tissues. SIRT1 immunostaining localized both in the nucleus (145/300, 48.3%) and the cytoplasm (70/300, 23.3%), and the overexpression of nuclear SIRT1 was positively related to the overexpression of P53 and YAP2. Survival analysis showed that nuclear SIRT1, P53 and YAP2 overexpression predicted poor overall survival while cytoplasmic SIRT1 overexpression predicted longer overall survival. Multivariate analysis showed nuclear SIRT1 and P53 overexpression as independent tumor promoters while cytoplasmic SIRT1 overexpression as an independent tumor suppressor.
  
  
  CONCLUSIONS
  SIRT1 was overexpressed in HCC and the expression was positively related to P53 and YAP2 expression. As the nuclear SIRT1 functions as a tumor promoter and cytoplasmic SIRT1 functions as a tumor suppressor, the role of SIRT1 in HCC should be reconsidered.
authors.affiliation: Department of General Surgery, China-Japan Union Hospital of Jilin University, Changchun, China.
authors.forename: Shijun
authors.initials: S
authors.lastname: Song
authors.affiliation: Department of Pain Management, China-Japan Union Hospital of Jilin University, Changchun, China.
authors.forename: Min
authors.initials: M
authors.lastname: Luo
authors.affiliation: Department of General Surgery, China-Japan Union Hospital of Jilin University, Changchun, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Song
authors.affiliation: Department of Pain Management, China-Japan Union Hospital of Jilin University, Changchun, China.
authors.forename: Tianji
authors.initials: T
authors.lastname: Liu
authors.affiliation: Department of General Surgery, China-Japan Union Hospital of Jilin University, Changchun, China.
authors.forename: Haishan
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Department of General Surgery, China-Japan Union Hospital of Jilin University, Changchun, China.
authors.forename: Zhongshi
authors.initials: Z
authors.lastname: Xie
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics / Q000378:metabolism*; D000293:Adolescent; D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor; D006528:Carcinoma, Hepatocellular / Q000235:genetics / Q000473:pathology*; D002681:China; D005260:Female; D005500:Follow-Up Studies; D015972:Gene Expression Regulation, Neoplastic / Q000502:physiology; D006801:Humans; D007150:Immunohistochemistry; D053208:Kaplan-Meier Estimate; D008113:Liver Neoplasms / Q000235:genetics / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D015999:Multivariate Analysis; D010750:Phosphoproteins / Q000235:genetics / Q000378:metabolism*; D012189:Retrospective Studies; D056564:Sirtuin 1 / Q000235:genetics / Q000378:metabolism*; D016019:Survival Analysis; D046888:Tissue Array Analysis; D014157:Transcription Factors; D016159:Tumor Suppressor Protein p53 / Q000235:genetics / Q000378:metabolism*; D000091102:YAP-Signaling Proteins; D055815:Young Adult
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D014408:Biomarkers, Tumor; D010750:Phosphoproteins; D014157:Transcription Factors; D016159:Tumor Suppressor Protein p53; D000091102:YAP-Signaling Proteins; C088374:YAP1 protein, human; D056564:Sirtuin 1