title: Molecular genotyping of herbicide resistance in P. minor: ACCase resistance.
abstract: Little seed canary grass (Phalaris minor Retz.) populations resistant to herbicides that inhibit acetyl-CoA carboxylase (ACCase) represent an increasingly important weed control problem in northern India. The objective of this study was to develop DNA-based markers to differentiate herbicide-resistant and herbicide-susceptible population of P. minor. Primers were designed to amplify the conserved region carrying two reported mutations Trp2027 to Cys and Ile2041 to Asn conferring ACCase inhibitor resistance in several grass weeds and subjected to single-strand conformational polymorphism (SSCP) to detect the mutations. Five distinctive electrophoretic patterns on non-denaturing PAGE were observed, and four patterns were found to be associated with ACCase herbicide resistance in P. minor. The PCR-SSCP test developed in this study confirmed 17 resistant populations to contain mutations in CT domain of ACCase gene. This is the first report of rapid and easy molecular diagnosis of ACCase herbicide-resistant and herbicide-sensitive population of P. minor through PCR-SSCP analysis.
authors.affiliation: ICAR - Directorate of Wheat Research, Karnal, 132001, India, rajenderkhokhar@yahoo.com.
authors.forename: Rajender
authors.initials: R
authors.lastname: Singh
authors.forename: Davinder
authors.initials: D
authors.lastname: Sharma
authors.forename: Nishu
authors.initials: N
authors.lastname: Raghav
authors.forename: Rajender Singh
authors.initials: RS
authors.lastname: Chhokar
authors.forename: Indu
authors.initials: I
authors.lastname: Sharma
mesh_terms: D000103:Acetyl-CoA Carboxylase / Q000737:chemistry / Q000235:genetics*; D000595:Amino Acid Sequence; D060005:Genotyping Techniques; D053209:Herbicide Resistance / Q000235:genetics*; D008969:Molecular Sequence Data; D031783:Phalaris / Q000201:enzymology* / Q000235:genetics*; D057685:Plant Weeds / Q000201:enzymology / Q000235:genetics; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational; D017434:Protein Structure, Tertiary; D016415:Sequence Alignment
chemical_list: D000103:Acetyl-CoA Carboxylase