title: Effective and selective cell retention and recovery from whole blood by electroactive thin films.
abstract: Hematogenous metastatic spread causes most cancer patient deaths. Because circulating tumor cells (CTCs) are highly relevant to early metastatic spread, the capture or detection of these cells provides a diagnostic tool for patients with metastatic conditions. Herein, we demonstrate a programmable electroactive multilayered material platform with a smart electrically induced "switch" that captures CTCs from biological plasma with high efficiency and releases the captured cells flexibly. The released cells are still viable and proliferative, which facilitates the detection of trace levels of CTCs by amplification. Furthermore, the inherent rough characteristics of the nanoparticle-composed interface can promote capture efficiency and cell purification by integration with a simple microfluidic device. This elegant, inexpensive, and versatile platform for cell sorting and enrichment makes subsequent molecular and cell biological analysis achievable. The strategy has broad implications for favoring fundamental cancer biology research, for the diagnosis and monitoring of cancer individually, and for advanced intervention based on blood purification.
authors.affiliation: Cancer Institute (Key Laboratory of Cancer Prevention and Intervention, National Ministry of Education, Key Laboratory of Molecular Biology in Medical Sciences, Zhejiang Province), The Second Affiliated Hospital, School of Medicine, Zhejiang University , Hangzhou, 310009, China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Xiao
authors.forename: Hangyu
authors.initials: H
authors.lastname: Zhou
authors.forename: Nanxia
authors.initials: N
authors.lastname: Xuan
authors.forename: Meng
authors.initials: M
authors.lastname: Cheng
authors.forename: Yuefeng
authors.initials: Y
authors.lastname: Rao
authors.forename: Yan
authors.initials: Y
authors.lastname: Luo
authors.forename: Ben
authors.initials: B
authors.lastname: Wang
authors.forename: Ruikang
authors.initials: R
authors.lastname: Tang
mesh_terms: D049109:Cell Proliferation; D002469:Cell Separation / Q000379:methods*; D006801:Humans; D046210:Microfluidic Analytical Techniques; D053758:Nanoparticles / Q000737:chemistry*; D009362:Neoplasm Metastasis; D009369:Neoplasms / Q000097:blood*; D009360:Neoplastic Cells, Circulating
keywords: cell sorting; circulating tumor cells; controlled release; electroactive; multilayer films