title: Electron capture dissociation in a branched radio-frequency ion trap.
abstract: We have developed a high-throughput electron capture dissociation (ECD) device coupled to a quadrupole time-of-flight mass spectrometer using novel branched radio frequency ion trap architecture. With this device, a low-energy electron beam can be injected orthogonally into the analytical ion beam with independent control of both the ion and electron beams. While ions and electrons can interact in a "flow-through" mode, we observed a large enhancement in ECD efficiency by introducing a short ion trapping period at the region of ion and electron beam intersection. This simultaneous trapping mode still provides up to five ECD spectra per second while operating in an information-dependent acquisition workflow. Coupled to liquid chromatography (LC), this LC-ECD workflow provides good sequence coverage for both trypsin and Lys C digests of bovine serum albumin, providing ECD spectra for doubly charged precursor ions with very good efficiency.
authors.affiliation: AB Sciex , 71 Four Valley Drive, Concord, Ontario, L4K 4V8, Canada.
authors.forename: Takashi
authors.initials: T
authors.lastname: Baba
authors.forename: J Larry
authors.initials: JL
authors.lastname: Campbell
authors.forename: J C Yves
authors.initials: JC
authors.lastname: Le Blanc
authors.forename: James W
authors.initials: JW
authors.lastname: Hager
authors.forename: Bruce A
authors.initials: BA
authors.lastname: Thomson
mesh_terms: D000818:Animals; D002417:Cattle; D002853:Chromatography, Liquid / Q000379:methods*; D004583:Electrons; D007477:Ions; D008239:Lysine / Q000737:chemistry; D011846:Radio Waves; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D017550:Spectroscopy, Fourier Transform Infrared; D014357:Trypsin / Q000737:chemistry
chemical_list: D007477:Ions; D012710:Serum Albumin, Bovine; D014357:Trypsin; D008239:Lysine