title: Co-cultivation of fungal and microalgal cells as an efficient system for harvesting microalgal cells, lipid production and wastewater treatment.
abstract: The challenges which the large scale microalgal industry is facing are associated with the high cost of key operations such as harvesting, nutrient supply and oil extraction. The high-energy input for harvesting makes current commercial microalgal biodiesel production economically unfeasible and can account for up to 50% of the total cost of biofuel production. Co-cultivation of fungal and microalgal cells is getting increasing attention because of high efficiency of bio-flocculation of microalgal cells with no requirement for added chemicals and low energy inputs. Moreover, some fungal and microalgal strains are well known for their exceptional ability to purify wastewater, generating biomass that represents a renewable and sustainable feedstock for biofuel production. We have screened the flocculation efficiency of the filamentous fungus A. fumigatus against 11 microalgae representing freshwater, marine, small (5 µm), large (over 300 µm), heterotrophic, photoautotrophic, motile and non-motile strains. Some of the strains are commercially used for biofuel production. Lipid production and composition were analysed in fungal-algal pellets grown on media containing alternative carbon, nitrogen and phosphorus sources contained in wheat straw and swine wastewater, respectively. Co-cultivation of algae and A. fumigatus cells showed additive and synergistic effects on biomass production, lipid yield and wastewater bioremediation efficiency. Analysis of fungal-algal pellet's fatty acids composition suggested that it can be tailored and optimised through co-cultivating different algae and fungi without the need for genetic modification.
authors.affiliation: Royal Melbourne Institute of Technology University, School of Applied Sciences, 3083 Bundoora, VIC, Australia.
authors.forename: Digby
authors.initials: D
authors.lastname: Wrede
authors.affiliation: Royal Melbourne Institute of Technology University, School of Applied Sciences, 3083 Bundoora, VIC, Australia.
authors.forename: Mohamed
authors.initials: M
authors.lastname: Taha
authors.affiliation: Royal Melbourne Institute of Technology University, School of Applied Sciences, 3083 Bundoora, VIC, Australia.
authors.forename: Ana F
authors.initials: AF
authors.lastname: Miranda
authors.affiliation: Royal Melbourne Institute of Technology University, School of Applied Sciences, 3083 Bundoora, VIC, Australia.
authors.forename: Krishna
authors.initials: K
authors.lastname: Kadali
authors.affiliation: Royal Melbourne Institute of Technology University, School of Applied Sciences, 3083 Bundoora, VIC, Australia.
authors.forename: Trevor
authors.initials: T
authors.lastname: Stevenson
authors.affiliation: Royal Melbourne Institute of Technology University, School of Applied Sciences, 3083 Bundoora, VIC, Australia.
authors.forename: Andrew S
authors.initials: AS
authors.lastname: Ball
authors.affiliation: Royal Melbourne Institute of Technology University, School of Applied Sciences, 3083 Bundoora, VIC, Australia.
authors.forename: Aidyn
authors.initials: A
authors.lastname: Mouradov
mesh_terms: D001232:Aspergillus fumigatus / Q000166:cytology / Q000254:growth & development / Q000378:metabolism; D056804:Biofuels / Q000382:microbiology; D018533:Biomass; D018920:Coculture Techniques / Q000379:methods; D005658:Fungi / Q000166:cytology* / Q000254:growth & development / Q000378:metabolism; D007218:Industrial Microbiology / Q000379:methods; D008055:Lipids / Q000096:biosynthesis*; D058086:Microalgae / Q000166:cytology* / Q000254:growth & development / Q000378:metabolism; D015203:Reproducibility of Results; D014865:Waste Disposal, Fluid / Q000379:methods*; D062065:Wastewater / Q000382:microbiology
chemical_list: D056804:Biofuels; D008055:Lipids; D062065:Wastewater