title: NMDA receptor-dependent metaplasticity by high-frequency magnetic stimulation.
abstract: High-frequency magnetic stimulation (HFMS) can elicit N-methyl-D-aspartate (NMDA) receptor-dependent long-term potentiation (LTP) at Schaffer collateral-CA1 pyramidal cell synapses. Here, we investigated the priming effect of HFMS on the subsequent magnitude of electrically induced LTP in the CA1 region of rat hippocampal slices using field excitatory postsynaptic potential (fEPSP) recordings. In control slices, electrical high-frequency conditioning stimulation (CS) could reliably induce LTP. In contrast, the same CS protocol resulted in long-term depression when HFMS was delivered to the slice 30 min prior to the electrical stimulation. HFMS-priming was diminished when applied in the presence of the metabotropic glutamate receptor antagonists (RS)-α-methylserine-O-phosphate (MSOP) and (RS)-α-methyl-4-carboxyphenylglycine (MCPG). Moreover, when HFMS was delivered in the presence of the NMDA receptor-antagonist D-2-amino-5-phosphonovalerate (50 µM), CS-induced electrical LTP was again as high as under control conditions in slices without priming. These results demonstrate that HFMS significantly reduced the propensity of subsequent electrical LTP and show that both metabotropic glutamate and NMDA receptor activation were involved in this form of HFMS-induced metaplasticity.
authors.affiliation: Oscar Langendorff Institute of Physiology, University of Rostock, Gertrudenstraße 9, 18057 Rostock, Germany ; Center for Life Sciences, Nazarbayev University, 53 Kabanbay Batyr Avenue, Astana 010000, Kazakhstan.
authors.forename: Tursonjan
authors.initials: T
authors.lastname: Tokay
authors.affiliation: Oscar Langendorff Institute of Physiology, University of Rostock, Gertrudenstraße 9, 18057 Rostock, Germany.
authors.forename: Timo
authors.initials: T
authors.lastname: Kirschstein
authors.affiliation: Oscar Langendorff Institute of Physiology, University of Rostock, Gertrudenstraße 9, 18057 Rostock, Germany.
authors.forename: Marco
authors.identifier: 0000-0002-6320-441X
authors.initials: M
authors.lastname: Rohde
authors.affiliation: Institute of Sport Sciences, University of Rostock, Ulmenstraße 69, 18057 Rostock, Germany.
authors.forename: Volker
authors.initials: V
authors.lastname: Zschorlich
authors.affiliation: Oscar Langendorff Institute of Physiology, University of Rostock, Gertrudenstraße 9, 18057 Rostock, Germany.
authors.forename: Rüdiger
authors.initials: R
authors.lastname: Köhling
mesh_terms: D000818:Animals; D056547:CA1 Region, Hippocampal / Q000502:physiology*; D004558:Electric Stimulation; D017774:Long-Term Potentiation; D060526:Magnetic Fields; D008297:Male; D051381:Rats; D016194:Receptors, N-Methyl-D-Aspartate / Q000502:physiology
chemical_list: D016194:Receptors, N-Methyl-D-Aspartate