title: Cytoprotective effect of short-term pretreatment with proanthocyanidin on human gingival fibroblasts exposed to harsh environmental conditions.
abstract: Our previous study showed that exposing mouse fibroblasts to proanthocyanidin (PA) for only 1 min accelerated cell proliferation in a concentration-dependent manner. In this study, exposing human gingival fibroblasts (HGFs) to PA for 1 min similarly accelerated the proliferative response of the cells. Besides the accelerated proliferative response, PA showed a cytoprotective effect on HGFs exposed to harsh environmental conditions; short-term exposure of HGFs in the mitotic phase to pure water or physiological saline resulted in a lower recovery of viable cells. Pretreatment and concomitant treatment with PA improved the low recovery of cells exposed to pure water or physiological saline. In addition, HGFs exposed to PA for 1 min proliferated well even after being cultured in serum-free medium. In 100% confluent HGFs, being cultured in serum-free medium resulted in a high intracellular reactive oxygen species (ROS) level, but pretreatment with PA prevented the cells from increasing intracellular ROS. Thus, the results suggest that a short-term PA treatment exerts cytoprotective effects on HGFs exposed to harsh environmental conditions by improving the intracellular oxidative stress response.
authors.affiliation: Tohoku University Graduate School of Dentistry, Aoba-ku, Sendai, Japan.
authors.forename: Michiko
authors.initials: M
authors.lastname: Kurauchi
authors.affiliation: Tohoku University Graduate School of Dentistry, Aoba-ku, Sendai, Japan.
authors.forename: Yoshimi
authors.initials: Y
authors.lastname: Niwano
authors.affiliation: Tohoku University Graduate School of Dentistry, Aoba-ku, Sendai, Japan.
authors.forename: Midori
authors.initials: M
authors.lastname: Shirato
authors.affiliation: Tohoku University Graduate School of Dentistry, Aoba-ku, Sendai, Japan.
authors.forename: Taro
authors.initials: T
authors.lastname: Kanno
authors.affiliation: Tohoku University Graduate School of Dentistry, Aoba-ku, Sendai, Japan.
authors.forename: Keisuke
authors.initials: K
authors.lastname: Nakamura
authors.affiliation: Tohoku University Graduate School of Dentistry, Aoba-ku, Sendai, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Egusa
authors.affiliation: Tohoku University Graduate School of Dentistry, Aoba-ku, Sendai, Japan.
authors.forename: Keiichi
authors.initials: K
authors.lastname: Sasaki
mesh_terms: D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D016895:Culture Media, Serum-Free / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D005347:Fibroblasts / Q000187:drug effects* / Q000378:metabolism; D005881:Gingiva / Q000166:cytology*; D006801:Humans; D044945:Proanthocyanidins / Q000494:pharmacology*; D020011:Protective Agents / Q000494:pharmacology; D017382:Reactive Oxygen Species / Q000378:metabolism; D012965:Sodium Chloride / Q000494:pharmacology; D013997:Time Factors; D014867:Water / Q000494:pharmacology
chemical_list: D016895:Culture Media, Serum-Free; D044945:Proanthocyanidins; D020011:Protective Agents; D017382:Reactive Oxygen Species; D014867:Water; C013221:proanthocyanidin; D012965:Sodium Chloride