title: Evidence of a tomato spotted wilt virus resistance-breaking strain originated through natural reassortment between two evolutionary-distinct isolates.
abstract: A Tsw resistance-breaking tomato spotted wilt virus field isolate (TSWV-p331) found in northern Italy originated via reassortment from two evolutionary distinct TSWV strains, as revealed by recombination and phylogenetic analysis. Compared to the closest isolate present in the database, p331 NSs protein carries an unusually high number of amino acid substitutions, but no differences in the nucleocapsid protein. Despite these substitutions, p331 NSs is a potent silencing suppressor. As shown by phylogenetic analyses of TSWV nucleocapsid sequences collected over fifteen years, one likely p331 parental lineage has never been detected in northern Italy, allowing speculations on the origin of TSWV-p331.
authors.affiliation: Istituto per la Protezione Sostenibile delle Piante, CNR, Strada delle Cacce 73, 10135 Torino, Italy; Department of Plant Pathology and Environmental Microbiology, Pennsylvania State University, University Park, PA 16802, USA. Electronic address: pzm150@psu.edu.
authors.forename: P
authors.initials: P
authors.lastname: Margaria
authors.affiliation: Istituto per la Protezione Sostenibile delle Piante, CNR, Strada delle Cacce 73, 10135 Torino, Italy. Electronic address: m.ciuffo@ivv.cnr.it.
authors.forename: M
authors.initials: M
authors.lastname: Ciuffo
authors.affiliation: Department of Plant Pathology and Environmental Microbiology, Pennsylvania State University, University Park, PA 16802, USA. Electronic address: czr2@psu.edu.
authors.forename: C
authors.initials: C
authors.lastname: Rosa
authors.affiliation: Istituto per la Protezione Sostenibile delle Piante, CNR, Strada delle Cacce 73, 10135 Torino, Italy. Electronic address: m.turina@ivv.cnr.it.
authors.forename: M
authors.initials: M
authors.lastname: Turina
mesh_terms: D060467:Disease Resistance; D019143:Evolution, Molecular; D016679:Genome, Viral; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology*; D012367:RNA, Viral; D016865:Reassortant Viruses; D011995:Recombination, Genetic; D017865:Tospovirus / Q000145:classification / Q000235:genetics*
chemical_list: D012367:RNA, Viral
keywords: Phylogeny; Reassortment; Silencing suppressor; Tospovirus