title: Switching of bacterial adhesion to a glycosylated surface by reversible reorientation of the carbohydrate ligand.
abstract: The surface recognition in many biological systems is guided by the interaction of carbohydrate-specific proteins (lectins) with carbohydrate epitopes (ligands) located within the unordered glycoconjugate layer (glycocalyx) of cells. Thus, for recognition, the respective ligand has to reorient for a successful matching event. Herein, we present for the first time a model system, in which only the orientation of the ligand is altered in a controlled manner without changing the recognition quality of the ligand itself. The key for this orientational control is the embedding into an interfacial system and the use of a photoswitchable mechanical joint, such as azobenzene.
authors.affiliation: Department of Biochemistry, Chemistry, and Pharmacy, University of Frankfurt, Max-von-Laue-Strasse 7, 60438 Frankfurt/M. (Germany).
authors.forename: Theresa
authors.initials: T
authors.lastname: Weber
authors.forename: Vijayanand
authors.initials: V
authors.lastname: Chandrasekaran
authors.forename: Insa
authors.initials: I
authors.lastname: Stamer
authors.forename: Mikkel B
authors.initials: MB
authors.lastname: Thygesen
authors.forename: Andreas
authors.initials: A
authors.lastname: Terfort
authors.forename: Thisbe K
authors.initials: TK
authors.lastname: Lindhorst
mesh_terms: D018830:Adhesins, Escherichia coli / Q000737:chemistry / Q000378:metabolism; D001391:Azo Compounds / Q000737:chemistry; D001422:Bacterial Adhesion; D002241:Carbohydrates / Q000737:chemistry*; D004926:Escherichia coli / Q000502:physiology; D036781:Fimbriae Proteins / Q000737:chemistry / Q000378:metabolism; D006031:Glycosylation; D037102:Lectins / Q000737:chemistry* / Q000378:metabolism; D008024:Ligands; D008362:Mannosides / Q000737:chemistry; D013055:Spectrophotometry, Infrared
chemical_list: D018830:Adhesins, Escherichia coli; D001391:Azo Compounds; D002241:Carbohydrates; D037102:Lectins; D008024:Ligands; D008362:Mannosides; C058769:fimH protein, E coli; D036781:Fimbriae Proteins; C009850:azobenzene
keywords: azobenzene; carbohydrate recognition; glycocalyx; lectin; light-induced switching