title: The potential role of the glycoprotein osteoactivin/glycoprotein nonmetastatic melanoma protein B in pancreatic cancer.
abstract: OBJECTIVE
  Pancreatic ductal adenocarcinoma is still one of the deadliest solid cancers so the finding of new therapeutic approaches and novel targets are of utmost importance. Glycoprotein nonmetastatic melanoma protein B (GPNMB), initially termed glycoprotein nonmetastatic gene B and also named osteoactivin (OA), is a type 1 transmembrane protein that has been recently found to play a role in cancer cell proliferation, angiogenesis, and invasion. Due to its potential responsibility in cancer aggressiveness, the main objective of this work was to assess the role of GPNMB/OA in human pancreatic cancer.
  
  
  METHODS
  Using the human pancreatic cancer cell line Panc-1 in vitro, the effects of GPNMB on growth, proliferation, and invasion were tested by BrdU uptake, cell cycle and Annexin V-FITC analysis, RT-PCR, protein expression, and invasion chamber assays.
  
  
  RESULTS
  Our results showed that GPNMB/OA protein expression prevents cells from apoptosis-enhancing proliferation and represents a novel modulator of the invasion and metastasis in pancreatic cancer cells.
  
  
  CONCLUSIONS
  Due to its main membrane localization in cancer cells and its role in the aggressiveness of pancreatic cancer, GPNMB/OA could represent a novel targeted therapy for pancreatic cancer being attractive for antibody-based therapies.
authors.affiliation: From the *Department of Biochemistry and Molecular Biology I, University of Granada; †GENYO, Centre for Genomics and Oncological Research, Pfizer/University of Granada/Andalusian Regional Government, PTS Granada; and ‡Oncology Service, Virgen de las Nieves Hospital, Granada, Spain.
authors.forename: Carolina
authors.initials: C
authors.lastname: Torres
authors.forename: Ana
authors.initials: A
authors.lastname: Linares
authors.forename: Maria Jose
authors.initials: MJ
authors.lastname: Alejandre
authors.forename: Rogelio
authors.initials: R
authors.lastname: Palomino-Morales
authors.forename: Miguel
authors.initials: M
authors.lastname: Martin
authors.forename: Juan Ramon
authors.initials: JR
authors.lastname: Delgado
authors.forename: Joquina
authors.initials: J
authors.lastname: Martinez
authors.forename: Sonia
authors.initials: S
authors.lastname: Perales
mesh_terms: D017209:Apoptosis; D021441:Carcinoma, Pancreatic Ductal / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D045744:Cell Line, Tumor; D002462:Cell Membrane / Q000378:metabolism; D002465:Cell Movement; D049109:Cell Proliferation; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008562:Membrane Glycoproteins / Q000235:genetics / Q000378:metabolism*; D009361:Neoplasm Invasiveness; D010190:Pancreatic Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D015398:Signal Transduction; D013997:Time Factors; D014162:Transfection
chemical_list: C503042:GPNMB protein, human; D008562:Membrane Glycoproteins