title: Identification of Chlamydiae and Mycoplasma species in ruminants with ocular infections.
abstract: Infectious keratoconjunctivitis (IKC) is a highly contagious ocular inflammatory condition, which is often reported in domestic small and large ruminants. Multiple infectious aetiologies are reported to be involved, but information about the role of certain fastidious bacterial pathogens such as chlamydiae and mycoplasmas is limited in India. Hence, this study was performed to determine the role of these pathogens and their identification by molecular approach. A total of 53 samples from 31 ovine, 14 caprine and eight bovine having clinical symptoms were collected and tested using species-specific PCR tests for chlamydiae and mycoplasmas followed by nucleotide sequence analysis. The results showed 77.41, 14.29 and 25% samples were chlamydiae positive in ovine, caprine and bovine, respectively, whereas 41.93, 14.29 and 37.5% prevalence of mycoplasma infection was detected in ovine, caprine and bovines, respectively. Chlamydophila abortus, Chlamydophila psittaci, Mycoplasma arginini and Mycoplasma hyorhinis were detected from tested samples. To the best of our knowledge, this is the first time these species are identified in IKC cases from India. Coinfection of both chlamydial and mycoplasmal species was detected in eight IKC cases of ovine which suggest synergistic roles played by both chlamydiae and mycoplasma in IKC samples.
authors.affiliation: Department of Veterinary Microbiology, DGCN College of Veterinary and Animal Sciences, CSK HPKV, Palampur, India.
authors.forename: S
authors.initials: S
authors.lastname: Gupta
authors.affiliation: Department of Veterinary Microbiology, DGCN College of Veterinary and Animal Sciences, CSK HPKV, Palampur, India.
authors.forename: R
authors.initials: R
authors.lastname: Chahota
authors.affiliation: Department of Veterinary Microbiology, DGCN College of Veterinary and Animal Sciences, CSK HPKV, Palampur, India.
authors.forename: B
authors.initials: B
authors.lastname: Bhardwaj
authors.affiliation: Department of Veterinary Microbiology, DGCN College of Veterinary and Animal Sciences, CSK HPKV, Palampur, India.
authors.forename: P
authors.initials: P
authors.lastname: Priyanka
authors.affiliation: Department of Veterinary Microbiology, DGCN College of Veterinary and Animal Sciences, CSK HPKV, Palampur, India.
authors.forename: S
authors.initials: S
authors.lastname: Verma
authors.affiliation: Department of Veterinary Microbiology, DGCN College of Veterinary and Animal Sciences, CSK HPKV, Palampur, India.
authors.forename: M
authors.initials: M
authors.lastname: Sharma
mesh_terms: D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000453:epidemiology / Q000382:microbiology; D002689:Chlamydia / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D002690:Chlamydia Infections / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D005798:Genes, Bacterial; D015511:Goat Diseases / Q000382:microbiology; D006041:Goats; D007194:India; D007639:Keratoconjunctivitis, Infectious / Q000382:microbiology*; D009174:Mycoplasma / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D009175:Mycoplasma Infections / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D016133:Polymerase Chain Reaction; D015995:Prevalence; D012418:Ruminants / Q000382:microbiology*; D012756:Sheep; D012757:Sheep Diseases; D034561:Sheep, Domestic
keywords: bovine; caprine; chlamydia; keratoconjunctivitis; mycoplasma; ovine