title: Perinatal exposure to a low dose of bisphenol A impaired systemic cellular immune response and predisposes young rats to intestinal parasitic infection.
abstract: Perinatal exposure to the food contaminant bisphenol A (BPA) in rats induces long lasting adverse effects on intestinal immune homeostasis. This study was aimed at examining the immune response to dietary antigens and the clearance of parasites in young rats at the end of perinatal exposure to a low dose of BPA. Female rats were fed with BPA [5 µg/kg of body weight/day] or vehicle from gestational day 15 to pup weaning. Juvenile female offspring (day (D)25) were used to analyze immune cell populations, humoral and cellular responses after oral tolerance or immunization protocol to ovalbumin (OVA), and susceptibility to infection by the intestinal nematode Nippostrongylus brasiliensis (N. brasiliensis). Anti-OVA IgG titers following either oral tolerance or immunization were not affected after BPA perinatal exposure, while a sharp decrease in OVA-induced IFNγ secretion occurred in spleen and mesenteric lymph nodes (MLN) of OVA-immunized rats. These results are consistent with a decreased number of helper T cells, regulatory T cells and dendritic cells in spleen and MLN of BPA-exposed rats. The lack of cellular response to antigens questioned the ability of BPA-exposed rats to clear intestinal infections. A 1.5-fold increase in N. brasiliensis living larvae was observed in the intestine of BPA-exposed rats compared to controls due to an inappropriate Th1/Th2 cytokine production in infected jejunal tissues. These results show that perinatal BPA exposure impairs cellular response to food antigens, and increases susceptibility to intestinal parasitic infection in the juveniles. This emphasized the maturing immune system during perinatal period highly sensitive to low dose exposure to BPA, altering innate and adaptative immune response capacities in early life.
authors.affiliation: Neuro-Gastroenterology and Nutrition INRA, UMR1331 Toxalim, Research Centre in Food Toxicology, Toulouse, France.
authors.forename: Sandrine
authors.initials: S
authors.lastname: Ménard
authors.affiliation: Intestinal Development, Xenobiotics & ImmunoToxicology INRA, UMR1331 Toxalim, Research Centre in Food Toxicology, Toulouse, France.
authors.forename: Laurence
authors.initials: L
authors.lastname: Guzylack-Piriou
authors.affiliation: Neuro-Gastroenterology and Nutrition INRA, UMR1331 Toxalim, Research Centre in Food Toxicology, Toulouse, France.
authors.forename: Corinne
authors.initials: C
authors.lastname: Lencina
authors.affiliation: Neuro-Gastroenterology and Nutrition INRA, UMR1331 Toxalim, Research Centre in Food Toxicology, Toulouse, France.
authors.forename: Mathilde
authors.initials: M
authors.lastname: Leveque
authors.affiliation: Neuro-Gastroenterology and Nutrition INRA, UMR1331 Toxalim, Research Centre in Food Toxicology, Toulouse, France.
authors.forename: Manon
authors.initials: M
authors.lastname: Naturel
authors.affiliation: Intestinal Development, Xenobiotics & ImmunoToxicology INRA, UMR1331 Toxalim, Research Centre in Food Toxicology, Toulouse, France.
authors.forename: Soraya
authors.initials: S
authors.lastname: Sekkal
authors.affiliation: Neuro-Gastroenterology and Nutrition INRA, UMR1331 Toxalim, Research Centre in Food Toxicology, Toulouse, France.
authors.forename: Cherryl
authors.initials: C
authors.lastname: Harkat
authors.affiliation: Intestinal Development, Xenobiotics & ImmunoToxicology INRA, UMR1331 Toxalim, Research Centre in Food Toxicology, Toulouse, France.
authors.forename: Eric
authors.initials: E
authors.lastname: Gaultier
authors.affiliation: Neuro-Gastroenterology and Nutrition INRA, UMR1331 Toxalim, Research Centre in Food Toxicology, Toulouse, France.
authors.forename: Maïwenn
authors.initials: M
authors.lastname: Olier
authors.affiliation: INRA, UMR1331 Toxalim, Research Centre in Food Toxicology, Toulouse, France.
authors.forename: Raphael
authors.initials: R
authors.lastname: Garcia-Villar
authors.affiliation: Neuro-Gastroenterology and Nutrition INRA, UMR1331 Toxalim, Research Centre in Food Toxicology, Toulouse, France.
authors.forename: Vassilia
authors.initials: V
authors.lastname: Theodorou
authors.affiliation: Intestinal Development, Xenobiotics & ImmunoToxicology INRA, UMR1331 Toxalim, Research Centre in Food Toxicology, Toulouse, France.
authors.forename: Eric
authors.initials: E
authors.lastname: Houdeau
mesh_terms: D000818:Animals; D001559:Benzhydryl Compounds / Q000008:administration & dosage / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005260:Female; D007111:Immunity, Cellular / Q000187:drug effects*; D007411:Intestinal Diseases, Parasitic / Q000276:immunology*; D018811:Maternal Exposure; D010636:Phenols / Q000008:administration & dosage / Q000494:pharmacology*; D011247:Pregnancy; D051381:Rats; D017208:Rats, Wistar; D000098826:Bisphenol A Compounds
chemical_list: D001559:Benzhydryl Compounds; D010636:Phenols; C006780:bisphenol A; D000098826:Bisphenol A Compounds