title: In vitro and in vivo replication of a chemically synthesized consensus genome of hepatitis B virus genotype B.
abstract: Hepatitis B virus (HBV) genotypes vary in their geographical distribution and virological features. Previous investigations have shown that HBV genotype B is a predominant HBV genotype in China. Studies on HBV concerning different isolates frequently meet the question about the HBV reference strain and its representativeness. Although HBV consensus sequences can be generated easily by sequence alignment, they may not exist in nature or could not usually be isolated from patient samples. Thus, the construction of a consensus HBV genome has been proposed. In this study, an HBV genotype B consensus sequence was established by comparing 42 full-length HBV genotype B sequences and the genome was generated by chemical synthesis. This consensus genome was fully replication competent by in vitro transfection into hepatoma cells. The plasmid pHBV1.3B carrying a 1.3× full-length HBV consensus genome was hydrodynamically injected into Balb/c mice. HBsAg, anti-HBs, HBeAg, anti-HBe, and anti-HBc detection indicated expression and replication of this HBV genome in mice, similar to other HBV isolates. This approach represents a strategy to design and create consensus HBV genomes for future studies.
authors.affiliation: Department of Infectious Diseases, The First Affiliated Hospital, Anhui Medical University, Hefei 230022, China; Department of Gastroenterology and Hepatology, University Hospital of Essen, University Duisburg-Essen, Essen 45122, Germany; Institute of Virology, University Hospital of Essen, University Duisburg-Essen, Essen 45122, Germany.
authors.forename: Zhenhua
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Institute of Virology, University Hospital of Essen, University Duisburg-Essen, Essen 45122, Germany.
authors.forename: Jianbo
authors.initials: J
authors.lastname: Xia
authors.affiliation: Department of Infectious Diseases, The First Affiliated Hospital, Anhui Medical University, Hefei 230022, China.
authors.forename: Binghu
authors.initials: B
authors.lastname: Sun
authors.affiliation: Department of Infectious Diseases, The First Affiliated Hospital, Anhui Medical University, Hefei 230022, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Dai
authors.affiliation: Department of Infectious Diseases, The First Affiliated Hospital, Anhui Medical University, Hefei 230022, China.
authors.forename: Xu
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Gastroenterology and Hepatology, University Hospital of Essen, University Duisburg-Essen, Essen 45122, Germany.
authors.forename: Joerg F
authors.initials: JF
authors.lastname: Schlaak
authors.affiliation: Institute of Virology, University Hospital of Essen, University Duisburg-Essen, Essen 45122, Germany. Electronic address: Mengji.lu@uni-due.de.
authors.forename: Mengji
authors.initials: M
authors.lastname: Lu
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D016384:Consensus Sequence; D004279:DNA, Viral / Q000138:chemical synthesis* / Q000235:genetics; D016679:Genome, Viral; D005838:Genotype; D006510:Hepatitis B Antibodies / Q000097:blood; D006514:Hepatitis B Surface Antigens / Q000097:blood; D006515:Hepatitis B virus / Q000235:genetics / Q000502:physiology*; D022781:Hepatocytes / Q000821:virology; D006801:Humans; D008297:Male; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA; D014779:Virus Replication
chemical_list: D004279:DNA, Viral; D006510:Hepatitis B Antibodies; D006514:Hepatitis B Surface Antigens
keywords: Consensus genome; Genotype; Hepatitis B virus; Replication