title: Selection of Phage Display Peptides Targeting Human Pluripotent Stem Cell-Derived Progenitor Cell Lines.
abstract: The ability of human pluripotent stem cells (hPS) to both self-renew and differentiate into virtually any cell type makes them a promising source of cells for cell-based regenerative therapies. However, stem cell identity, purity, and scalability remain formidable challenges that need to be overcome for translation of pluripotent stem cell research into clinical applications. Directed differentiation from hPS cells is inefficient and residual contamination with pluripotent cells that have the potential to form tumors remains problematic. The derivation of scalable (self-renewing) embryonic progenitor stem cell lines offers a solution because they are well defined and clonally pure. Clonally pure progenitor stem cell lines also provide a means for identifying cell surface targeting reagents that are useful for identification, tracking, and repeated derivation of the corresponding progenitor stem cell types from additional hPS cell sources. Such stem cell targeting reagents can then be applied to the manufacture of genetically diverse banks of human embryonic progenitor cell lines for drug screening, disease modeling, and cell therapy. Here we present methods to identify human embryonic progenitor stem cell targeting peptides by selection of phage display libraries on clonal embryonic progenitor cell lines and demonstrate their use for targeting quantum dots (Qdots) for stem cell labeling.
authors.affiliation: Mandala Biosciences, LLC, San Diego, CA, USA.
authors.forename: Paola A
authors.initials: PA
authors.lastname: Bignone
authors.affiliation: Mandala Biosciences, LLC, San Diego, CA, USA.
authors.forename: Rachel A
authors.initials: RA
authors.lastname: Krupa
authors.affiliation: BioTime Inc., 1301 Harbor Bay Parkway, Alameda, CA, 94502, USA.
authors.forename: Michael D
authors.initials: MD
authors.lastname: West
authors.affiliation: Mandala Biosciences, LLC, San Diego, CA, USA. dlarocca@biotimemail.com.
authors.forename: David
authors.initials: D
authors.lastname: Larocca
mesh_terms: D000595:Amino Acid Sequence; D001435:Bacteriophages / Q000235:genetics* / Q000302:isolation & purification; D019070:Cell Lineage; D002469:Cell Separation; D002999:Clone Cells; D053595:Embryonic Stem Cells / Q000166:cytology*; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology; D008969:Molecular Sequence Data; D010012:Osteogenesis; D019151:Peptide Library; D039904:Pluripotent Stem Cells / Q000166:cytology*; D045663:Quantum Dots; D044968:Regenerative Medicine / Q000379:methods
chemical_list: D019151:Peptide Library
keywords: Affinity selection; Phage display; Progenitor cells; Stem cells; Targeting peptides