title: Optimization of supercritical fluid extraction of oil from the fruit of Gardenia jasminoides and its antidepressant activity.
abstract: A response surface methodology was applied to optimize the variables affecting the supercritical ﬂuid carbon dioxide extraction of oil from the fruit of Gardenia jasminoides using the Box-Behnken design. The optimum extraction parameters were an extraction temperature of 49.94 °C, an extraction pressure of 29.89 MPa and an extraction time of 93.82 min. Through a GC/MS analysis, we revealed 16 major components of the oil extract, which showed potent antidepressant effects in both of two behavior despair models in mice: tail suspension test and forced swimming test. Our results suggest that the oil extract of Gardenia jasminoides prepared using the supercritical ﬂuid carbon dioxide extraction may contain effective constituents to be used for depression therapy.
authors.affiliation: Center for Translational Systems Biology and Neuroscience, School of Basic Biomedical Science, Nanjing University of Chinese Medicine, Nanjing 210023, China. jinaoduan1956@126.com.
authors.forename: Weiwei
authors.initials: W
authors.lastname: Tao
authors.affiliation: Center for Translational Systems Biology and Neuroscience, School of Basic Biomedical Science, Nanjing University of Chinese Medicine, Nanjing 210023, China. hailouzhang@163.com.
authors.forename: Hailou
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Center for Translational Systems Biology and Neuroscience, School of Basic Biomedical Science, Nanjing University of Chinese Medicine, Nanjing 210023, China. xuewenda@njutcm.edu.cn.
authors.forename: Wenda
authors.initials: W
authors.lastname: Xue
authors.affiliation: Center for Translational Systems Biology and Neuroscience, School of Basic Biomedical Science, Nanjing University of Chinese Medicine, Nanjing 210023, China. nicolight@163.com.
authors.forename: Li
authors.initials: L
authors.lastname: Ren
authors.affiliation: Center for Translational Systems Biology and Neuroscience, School of Basic Biomedical Science, Nanjing University of Chinese Medicine, Nanjing 210023, China. babaysummer@163.com.
authors.forename: Baomei
authors.initials: B
authors.lastname: Xia
authors.affiliation: Center for Translational Systems Biology and Neuroscience, School of Basic Biomedical Science, Nanjing University of Chinese Medicine, Nanjing 210023, China. aeon401@126.com.
authors.forename: Xin
authors.initials: X
authors.lastname: Zhou
authors.affiliation: Center for Translational Systems Biology and Neuroscience, School of Basic Biomedical Science, Nanjing University of Chinese Medicine, Nanjing 210023, China. wuhaoxin@sohu.com.
authors.forename: Haoxin
authors.initials: H
authors.lastname: Wu
authors.affiliation: National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine, Nanjing University of Chinese Medicine, Nanjing 210023, China. jinaoduan1956@126.com.
authors.forename: Jinao
authors.initials: J
authors.lastname: Duan
authors.affiliation: Center for Translational Systems Biology and Neuroscience, School of Basic Biomedical Science, Nanjing University of Chinese Medicine, Nanjing 210023, China. chengang@njutcm.edu.cn.
authors.forename: Gang
authors.initials: G
authors.lastname: Chen
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D000928:Antidepressive Agents / Q000494:pharmacology*; D025924:Chromatography, Supercritical Fluid / Q000379:methods*; D005638:Fruit / Q000737:chemistry*; D032062:Gardenia / Q000737:chemistry*; D007103:Immobilization; D007649:Ketamine / Q000494:pharmacology; D008297:Male; D051379:Mice; D010938:Plant Oils / Q000494:pharmacology*; D011312:Pressure; D013550:Swimming; D013696:Temperature; D013997:Time Factors
chemical_list: D000928:Antidepressive Agents; D010938:Plant Oils; D007649:Ketamine