title: Functionalized graphene sheets as immobilization matrix for Fenugreek β-amylase: enzyme kinetics and stability studies.
abstract: β-Amylase finds application in food and pharmaceutical industries. Functionalized graphene sheets were customised as a matrix for covalent immobilization of Fenugreek β-amylase using glutaraldehyde as a cross-linker. The factors affecting the process were optimized using Response Surface Methodology based Box-Behnken design of experiment which resulted in 84% immobilization efficiency. Scanning and Transmission Electron Microscopy (SEM, TEM) and Fourier Tansform Infrared (FTIR) spectroscopy were employed for the purpose of characterization of attachment of enzyme on the graphene. The enzyme kinetic studies were carried out for obtaining best catalytic performance and enhanced reusability. Optimum temperature remained unchanged, whereas optimum pH showed shift towards acidic range for immobilized enzyme. Increase in thermal stability of immobilized enzyme and non-toxic nature of functionalized graphene can be exploited for production of maltose in food and pharmaceutical industries.
authors.affiliation: School of Biotechnology, Faculty of Science, Banaras Hindu University, Varanasi, India.
authors.forename: Garima
authors.initials: G
authors.lastname: Srivastava
authors.affiliation: School of Biotechnology, Faculty of Science, Banaras Hindu University, Varanasi, India.
authors.forename: Kritika
authors.initials: K
authors.lastname: Singh
authors.affiliation: Nanoscience and Nanotechnology Unit, Department of Physics, Banaras Hindu University, Varanasi, India.
authors.forename: Mahe
authors.initials: M
authors.lastname: Talat
authors.affiliation: Nanoscience and Nanotechnology Unit, Department of Physics, Banaras Hindu University, Varanasi, India.
authors.forename: Onkar Nath
authors.initials: ON
authors.lastname: Srivastava
authors.affiliation: School of Biotechnology, Faculty of Science, Banaras Hindu University, Varanasi, India.
authors.forename: Arvind M
authors.initials: AM
authors.lastname: Kayastha
mesh_terms: D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism; D006108:Graphite / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008320:Maltose / Q000378:metabolism; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D017550:Spectroscopy, Fourier Transform Infrared; D013696:Temperature; D029910:Trigonella / Q000201:enzymology*; D001614:beta-Amylase / Q000737:chemistry / Q000378:metabolism*
chemical_list: D004800:Enzymes, Immobilized; D008320:Maltose; D006108:Graphite; D001614:beta-Amylase