title: From flab to fab: transforming surgical waste into an effective bioactive coating material.
abstract: Cellular events are regulated by the interaction between integrin receptors in the cell membrane and the extracellular matrix (ECM). Hence, ECM, as a material, can potentially play an instructive role in cell-material interactions. Currently, adipose tissue in the form of lipoaspirate is often discarded. Here, it is demonstrated how our chemical-free decellularization method could be used to obtain ECM from human lipoaspirate waste material. These investigations show that the main biological components are retained in the lipoaspirate-derived ECM (LpECM) material and that this LpECM material could subsequently be used as a coating material to confer bioactivity to an otherwise inert biodegradable material (i.e., polycaprolactone). Overall, lipoaspirate material, a complex blend of endogenous proteins, is effectively used a bioactive coating material. This work is an important stepping-stone towards the development of biohybrid scaffolds that contain cellular benefits without requiring the use of additional biologics based on commonly discarded lipoaspirate material.
authors.affiliation: Division of Materials Technology, School of Materials Science and Engineering, Nanyang Technological University, 50 Nanyang Avenue, 639798, Singapore.
authors.forename: Baiwen
authors.initials: B
authors.lastname: Luo
authors.forename: Shaojun
authors.initials: S
authors.lastname: Yuan
authors.forename: Selin Ee Min
authors.initials: SE
authors.lastname: Foo
authors.forename: Marcus Thien Chong
authors.initials: MT
authors.lastname: Wong
authors.forename: Thiam Chye
authors.initials: TC
authors.lastname: Lim
authors.forename: Nguan Soon
authors.initials: NS
authors.lastname: Tan
authors.forename: Cleo
authors.initials: C
authors.lastname: Choong
mesh_terms: D000328:Adult; D002448:Cell Adhesion / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D020099:Coated Materials, Biocompatible / Q000494:pharmacology*; D005109:Extracellular Matrix / Q000378:metabolism; D005260:Female; D006801:Humans; D015187:Lipectomy; D008506:Medical Waste; D008875:Middle Aged; D011091:Polyesters / Q000737:chemistry; D058105:Polymerization; D013234:Stem Cells / Q000166:cytology / Q000187:drug effects; D013499:Surface Properties; D014867:Water / Q000737:chemistry
chemical_list: D020099:Coated Materials, Biocompatible; D008506:Medical Waste; D011091:Polyesters; D014867:Water; C016240:polycaprolactone
keywords: extracellular matrix; matricellular proteins; polycaprolactone; polymerization; tissue engineering; waste management