title: Optimization of production, purification and lyophilisation of cellobiose dehydrogenase by Sclerotium rolfsii.
abstract: BACKGROUND
  The enzyme cellobiose dehydrogenase (CDH) can be used to oxidize lactose to lactobionic acid. As Sclerotium rolfsii is known to be a good producer of CDH, the aim of this paper was to simplify its production and secondly to systematically study its purification aiming for a high yield. Two preservation methods (freezing and freeze-drying) and the influence of several protectants were investigated.
  
  
  RESULTS
  Production of cellobiose dehydrogenase was optimized leading to a more simplified medium composition. Purification of the enzyme was evaluated by determining breakthrough profiles on different ion exchange (IEX) and hydrophobic interaction (HIC) materials with regard to buffer composition. Highest purification with an acceptable loss during the capture step using IEX was obtained with a Q Sepharose XL medium and a 100 mM sodium acetate buffer at pH 4.5. Subsequent purification using hydrophobic interaction chromatography was done at 1.1 M ammonium sulfate concentration. Purification was moderate, yielding a specific activity of 11.9 U/mg (56% yield). However, as could be shown in a preliminary experiment, purity of the obtained enzyme solution was sufficient for its intended use to oxidize lactose to lactobionic acid. Various sugars and sugar alcohols were investigated to study their protective effect during lyophilisation and freezing at -20 °C. Glucose and lactulose could be identified to have a high lyoprotective effect while loss of enzyme activity was high (77%) when using no additives.
  
  
  CONCLUSIONS
  By simplifying the cultivation medium of Sclerotium rolfsii, the costs of cellobiose dehydrogenase production could be reduced. Simultaneously, CDH production was increased by 21%. The production of lactobionic acid from lactose is possible using partially purified and unpurified enzyme. Storage at -20 °C using 50% (w/v) glycerol was considered to be most suited for preservation of the enzyme.
authors.affiliation: Anhalt University of Applied Sciences, Department of Applied Biosciences and Process Engineering, Bernburger Str. 55, 06366, Köthen, Germany. c.fischer@bwp.hs-anhalt.de.
authors.forename: Christin
authors.initials: C
authors.lastname: Fischer
authors.affiliation: Anhalt University of Applied Sciences, Department of Applied Biosciences and Process Engineering, Bernburger Str. 55, 06366, Köthen, Germany. a.krause@bwp.hs-anhalt.de.
authors.forename: Annett
authors.initials: A
authors.lastname: Krause
authors.affiliation: Anhalt University of Applied Sciences, Department of Applied Biosciences and Process Engineering, Bernburger Str. 55, 06366, Köthen, Germany. t.kleinschmidt@bwp.hs-anhalt.de.
authors.forename: Thomas
authors.initials: T
authors.lastname: Kleinschmidt
mesh_terms: D001487:Basidiomycota / Q000737:chemistry / Q000201:enzymology* / Q000235:genetics; D002237:Carbohydrate Dehydrogenases / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D004187:Disaccharides / Q000378:metabolism; D005656:Fungal Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D007700:Kinetics; D007785:Lactose / Q000378:metabolism; D013379:Substrate Specificity
chemical_list: D004187:Disaccharides; D005656:Fungal Proteins; C005608:lactobionic acid; D002237:Carbohydrate Dehydrogenases; C019859:cellobiose-quinone oxidoreductase; D007785:Lactose