title: MiR-145 acts as a metastasis suppressor by targeting metadherin in lung cancer.
abstract: MiR-145 has been reported to be downregulated in multiple tumors. It acts as a tumor suppressor in lung cancer. In this study, we investigated the potential effects of miR-145 on invasion and metastasis and the molecular mechanism in non-small cell lung cancer. MiR-145 was downregulated in the NSCLC specimens and significantly correlated with advanced clinical stage and lymph node metastasis. In addition, AEG-1/MTDH was a direct target of miR-145, and the expression of AEG-1/MTDH was inversely correlated with miR-145 expression in NSCLC tissues. Ectopic expression of miR-145 suppressed cell invasion and metastasis in NSCLC cells. AEG-1/MTDH overexpression partially reversed the suppressive effect of miR-145. These findings provide novel insights with potential therapeutic applications for the treatment of NSCLC.
authors.affiliation: Department of Cardiothoracic Surgery, Guangdong No.2 Provincial People Hospital, 1 Shiliugang Road, Guangzhou, 510317, Guangdong, China.
authors.forename: Mingzhi
authors.initials: M
authors.lastname: Wang
authors.forename: Jianhua
authors.initials: J
authors.lastname: Wang
authors.forename: Jianming
authors.initials: J
authors.lastname: Deng
authors.forename: Xuejun
authors.initials: X
authors.lastname: Li
authors.forename: Weiguang
authors.initials: W
authors.lastname: Long
authors.forename: Yongmei
authors.initials: Y
authors.lastname: Chang
mesh_terms: D015153:Blotting, Western; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics* / Q000473:pathology; D015815:Cell Adhesion Molecules / Q000096:biosynthesis*; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics*; D006801:Humans; D007150:Immunohistochemistry; D008175:Lung Neoplasms / Q000235:genetics* / Q000473:pathology; D008565:Membrane Proteins; D035683:MicroRNAs / Q000235:genetics*; D009361:Neoplasm Invasiveness / Q000235:genetics; D016601:RNA-Binding Proteins; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014162:Transfection
chemical_list: D015815:Cell Adhesion Molecules; C541010:MIRN145 microRNA, human; C483039:MTDH protein, human; D008565:Membrane Proteins; D035683:MicroRNAs; D016601:RNA-Binding Proteins