title: Effect of γ-irradiation on expression of tight and adherens junction protein mRNA on in vitro blood-brain barrier model.
abstract: We studied the effect of γ-irradiation on HUVEC endothelial cells co-cultured with allogeneic astrocytes. This 2D in vitro model of the blood-brain barrier has the same parameters as cerebral microvascular endothelial cells forming the blood-brain barrier and allows reproducing its functions in vivo. Dose-dependent changes in cell morphology and violation of monolayer integrity were observed. Real-time PCR and immunocytochemical analysis revealed changes in the expression of tight (ZO-1, claudin-5) and adherens junction protein (vascular endothelial cadherin, β-catenin) mRNA. Expression of tight and adherens junction proteins mRNA decreased in 2, 24, and 48 h after irradiation in doses of 2, 4, and 6 Gy. Significant dose-dependent changes were found only for β-catenin mRNA expression in 2 h after exposition. This model of blood-brain barrier in vitro can be used for studying the molecular mechanisms regulating permeability of cerebral endothelium under normal conditions and after pathological exposures, e.g. γ-irradiation.
authors.affiliation: V. P. Serbsky State Research Center for Social and Forensic Psychiatry, Moscow, Russia, zrkyana@gmail.com.
authors.forename: Ya A
authors.initials: YA
authors.lastname: Zorkina
authors.forename: N E
authors.initials: NE
authors.lastname: Volgina
authors.forename: G E
authors.initials: GE
authors.lastname: Gorlachev
authors.forename: P A
authors.initials: PA
authors.lastname: Mel'nikov
authors.forename: A V
authors.initials: AV
authors.lastname: Golanov
authors.forename: A A
authors.initials: AA
authors.lastname: Potapov
authors.forename: V P
authors.initials: VP
authors.lastname: Chekhonin
mesh_terms: D022005:Adherens Junctions / Q000378:metabolism*; D001812:Blood-Brain Barrier / Q000378:metabolism*; D002478:Cells, Cultured; D062507:Claudin-5 / Q000235:genetics / Q000378:metabolism; D018920:Coculture Techniques; D005720:Gamma Rays; D015870:Gene Expression / Q000528:radiation effects*; D061307:Human Umbilical Vein Endothelial Cells / Q000378:metabolism* / Q000528:radiation effects; D006801:Humans; D019108:Tight Junctions / Q000378:metabolism*; D062826:Zonula Occludens-1 Protein / Q000235:genetics / Q000378:metabolism; D051176:beta Catenin / Q000235:genetics / Q000378:metabolism
chemical_list: C107466:CLDN5 protein, human; C495270:CTNNB1 protein, human; D062507:Claudin-5; C569042:TJP1 protein, human; D062826:Zonula Occludens-1 Protein; D051176:beta Catenin