title: Effects of the Oxygen-Carrying Solution OxyVita C on the Cerebral Microcirculation and Systemic Blood Pressures in Healthy Rats.
abstract: The use of hemoglobin-based oxygen carriers (HBOC) as oxygen delivering therapies during hypoxic states has been hindered by vasoconstrictive side effects caused by depletion of nitric oxide (NO). OxyVita C is a promising oxygen-carrying solution that consists of a zero-linked hemoglobin polymer with a high molecular weight (~17 MDa). The large molecular weight is believed to prevent extravasation and limit NO scavenging and vasoconstriction. The aim of this study was to assess vasoactive effects of OxyVita C on systemic blood pressures and cerebral pial arteriole diameters. Anesthetized healthy rats received four intravenous (IV) infusions of an increasing dose of OxyVita C (2, 25, 50, 100 mg/kg) and hemodynamic parameters and pial arteriolar diameters were measured pre- and post-infusion. Normal saline was used as a volume-matched control. Systemic blood pressures increased (P ≤ 0.05) with increasing doses of OxyVita C, but not with saline. There was no vasoconstriction in small (<50 µm) and medium-sized (50-100 µm) pial arterioles in the OxyVita C group. In contrast, small and medium-sized pial arterioles vasoconstricted in the control group. Compared to saline, OxyVita C showed no cerebral vasoconstriction after any of the four doses evaluated in this rat model despite increases in blood pressure.
authors.affiliation: NeuroTrauma Department, Naval Medical Research Center (NMRC), Silver Spring, MD 20910, USA. rania.abutarboush@med.navy.mil.
authors.forename: Rania
authors.initials: R
authors.lastname: Abutarboush
authors.affiliation: NeuroTrauma Department, Naval Medical Research Center (NMRC), Silver Spring, MD 20910, USA. chioma.aligbe@med.navy.mil.
authors.forename: Chioma
authors.initials: C
authors.lastname: Aligbe
authors.affiliation: NeuroTrauma Department, Naval Medical Research Center (NMRC), Silver Spring, MD 20910, USA. georgina.pappas@quinnipiac.edu.
authors.forename: Georgina
authors.initials: G
authors.lastname: Pappas
authors.affiliation: NeuroTrauma Department, Naval Medical Research Center (NMRC), Silver Spring, MD 20910, USA. biswajit.saha@med.navy.mil.
authors.forename: Biswajit
authors.initials: B
authors.lastname: Saha
authors.affiliation: NeuroTrauma Department, Naval Medical Research Center (NMRC), Silver Spring, MD 20910, USA. francoise.arnaud@med.navy.mil.
authors.forename: Francoise
authors.initials: F
authors.lastname: Arnaud
authors.affiliation: NeuroTrauma Department, Naval Medical Research Center (NMRC), Silver Spring, MD 20910, USA. ashraful.haque@med.navy.mil.
authors.forename: Ashraful
authors.initials: A
authors.lastname: Haque
authors.affiliation: NeuroTrauma Department, Naval Medical Research Center (NMRC), Silver Spring, MD 20910, USA. chioma.aligbe@med.navy.mil.
authors.forename: Charles
authors.initials: C
authors.lastname: Auker
authors.affiliation: NeuroTrauma Department, Naval Medical Research Center (NMRC), Silver Spring, MD 20910, USA. richard.mccarron@med.navy.mil.
authors.forename: Richard
authors.initials: R
authors.lastname: McCarron
authors.affiliation: NeuroTrauma Department, Naval Medical Research Center (NMRC), Silver Spring, MD 20910, USA. anke.scultetus@med.navy.mil.
authors.forename: Anke
authors.initials: A
authors.lastname: Scultetus
authors.affiliation: NeuroTrauma Department, Naval Medical Research Center (NMRC), Silver Spring, MD 20910, USA. pfmoon@hotmail.com.
authors.forename: Paula
authors.initials: P
authors.lastname: Moon-Massat