title: Nonthrombogenic, biodegradable elastomeric polyurethanes with variable sulfobetaine content.
abstract: For applications where degradable polymers are likely to have extended blood contact, it is often important for these materials to exhibit high levels of thromboresistance. This can be achieved with surface modification approaches, but such modifications may be transient with degradation. Alternatively, polymer design can be altered such that the bulk polymer is thromboresistant and this is maintained with degradation. Toward this end a series of biodegradable, elastic polyurethanes (PESBUUs) containing different zwitterionic sulfobetaine (SB) content were synthesized from a polycaprolactone-diol (PCL-diol):SB-diol mixture (100:0, 75:25, 50:50, 25:75 and 0:100) reacted with diisocyanatobutane and chain extended with putrescine. The chemical structure, tensile mechanical properties, thermal properties, hydrophilicity, biodegradability, fibrinogen adsorption and thrombogenicity of the resulting polymers was characterized. With increased SB content some weakening in tensile properties occurred in wet conditions and enzymatic degradation also decreased. However, at higher zwitterionic molar ratios (50% and 75%) wet tensile strength exceeded 15 MPa and breaking strain was >500%. Markedly reduced thrombotic deposition was observed both before and after substantial degradation for both of these PESBUUs and they could be processed by electrospinning into a vascular conduit format with appropriate compliance properties. The mechanical and degradation properties as well as the acute in vitro thrombogenicity assessment suggest that these tunable polyurethanes could provide options appropriate for use in blood contacting applications where a degradable, elastomeric component with enduring thromboresistance is desired.
authors.affiliation: McGowan Institute for Regenerative Medicine, ‡Department of Surgery, ||Department of Bioengineering, and ▽Department of Chemical Engineering, University of Pittsburgh , Pittsburgh, Pennsylvania 15219, United States.
authors.forename: Sang-Ho
authors.initials: SH
authors.lastname: Ye
authors.forename: Yi
authors.initials: Y
authors.lastname: Hong
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Sakaguchi
authors.forename: Venkat
authors.initials: V
authors.lastname: Shankarraman
authors.forename: Samuel K
authors.initials: SK
authors.lastname: Luketich
authors.forename: Antonio
authors.initials: A
authors.lastname: D'Amore
authors.forename: William R
authors.initials: WR
authors.lastname: Wagner
mesh_terms: D020341:Absorbable Implants; D000818:Animals; D001622:Betaine / Q000031:analogs & derivatives* / Q000737:chemistry; D001672:Biocompatible Materials / Q000138:chemical synthesis* / Q000494:pharmacology*; D001777:Blood Coagulation / Q000187:drug effects* / Q000502:physiology; D002417:Cattle; D005343:Fibrinolytic Agents / Q000138:chemical synthesis / Q000494:pharmacology; D006244:Hardness; D008422:Materials Testing; D011140:Polyurethanes / Q000737:chemistry*; D013718:Tensile Strength
chemical_list: D001672:Biocompatible Materials; D005343:Fibrinolytic Agents; D011140:Polyurethanes; D001622:Betaine; C483727:sulfobetaine
keywords: biodegradable polyurethane; cardiovascular; sulfobetaine; thromboresistance; vascular graft; zwitterion