title: Sustained immobilization of growth factor proteins based on functionalized parylenes.
abstract: Protein molecules immobilized on biomaterial surfaces are performed based on oriented conjugation or replaced mimicking peptides. The sustainable immobilization of growth factor proteins using functionalized parylene coatings is demonstrated in this study. Site-specific and nonspecific immobilization approaches are realized to conjugate bone morphogenetic protein (BMP-2). The binding affinities and conformational changes of BMP-2 are confirmed by QCM and SPR characterizations. Osteoinduction of stem cells is examined by ALP activity on the BMP-2 modified surfaces. Finally, immobilizations and equally sustained biological functions of vascular endothelial growth factor (VEGF) and a mimicking peptide of KLTWQELYQLKYKG (QK) are also examined and confirmed.
authors.affiliation: Department of Chemical Engineering, National Taiwan University , Taipei 10617, Taiwan.
authors.forename: Yung-Chih
authors.initials: YC
authors.lastname: Chen
authors.forename: Ting-Pi
authors.initials: TP
authors.lastname: Sun
authors.forename: Chiao-Tzu
authors.initials: CT
authors.lastname: Su
authors.forename: Jyun-Ting
authors.initials: JT
authors.lastname: Wu
authors.forename: Chih-Yeh
authors.initials: CY
authors.lastname: Lin
authors.forename: Jiashing
authors.initials: J
authors.lastname: Yu
authors.forename: Chao-Wei
authors.initials: CW
authors.lastname: Huang
authors.forename: Chia-Jie
authors.initials: CJ
authors.lastname: Chen
authors.forename: Hsien-Yeh
authors.initials: HY
authors.lastname: Chen
mesh_terms: D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D036341:Intercellular Signaling Peptides and Proteins / Q000737:chemistry*; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism; D011108:Polymers / Q000737:chemistry*; D014992:Xylenes / Q000737:chemistry*
chemical_list: D036341:Intercellular Signaling Peptides and Proteins; D011108:Polymers; D014992:Xylenes; C011055:parylene
keywords: bioconjugation; biomaterial; growth factor; protein immobilization; surface modification