title: JcCBF2 gene from Jatropha curcas improves freezing tolerance of Arabidopsis thaliana during the early stage of stress.
abstract: High chilling-susceptibility is becoming the bottleneck for cultivation and commercialization of Jatropha curcas L. For insights to chilling resistance ability of this plant species, a cold response transcription factor, JcCBF2, was cloned and studied. It codes a 26 kDa protein, which contains all conserved motifs unique to the C-repeat binding factor (CBF) family and has high similarity to CBFs of Ricinus communis and Populus. Its transcripts express specifically in leaves of Jatropha at cold temperature. After transmitting the report vector, 35S::JcCBF2-GFP, into Arabidopsis thaliana, JcCBF2 protein is main detected in cell nucleus, being consistent to the nuclear orientation signal in its N-terminal. Compared to the control Arabidopsis, the frozen leaves of JcCBF2-overexpressed seedlings grow stronger with less malondialdehyde, smaller leaf conductivity and activer superoxide dismutase, showing their higher freezing tolerance. RT-PCR tests revealed that JcCBF2 functioned mainly at the early stage (0-6 h) of resistance events in Arabidopsis, and its transcripts reduced after 6 h. In addition, JcCBF2 could quickly regulate transcripts of some cold-responsive (COR) genes such as RD29A, COR105A and COR6.6, also during the early stage of frozen treatment. This study not only proves the chilling resistance roles of JcCBF2, but also presents a candidate gene engineering for improvement of chilling tolerance in J. curcas.
authors.affiliation: Key Laboratory of Bio-resources and Eco-environment of Ministry of Education, College of Life Sciences, Sichuan University, 24 South Section 1, 1st Ring Road, Chengdu, 610064, China.
authors.forename: Linghui
authors.initials: L
authors.lastname: Wang
authors.forename: Jihai
authors.initials: J
authors.lastname: Gao
authors.forename: Xiaobo
authors.initials: X
authors.lastname: Qin
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Shi
authors.forename: Lin
authors.initials: L
authors.lastname: Luo
authors.forename: Guozhen
authors.initials: G
authors.lastname: Zhang
authors.forename: Hongwu
authors.initials: H
authors.lastname: Yu
authors.forename: Chenyang
authors.initials: C
authors.lastname: Li
authors.forename: Minchao
authors.initials: M
authors.lastname: Hu
authors.forename: Qifan
authors.initials: Q
authors.lastname: Liu
authors.forename: Ying
authors.initials: Y
authors.lastname: Xu
authors.forename: Fang
authors.initials: F
authors.lastname: Chen
mesh_terms: D000064:Acclimatization / Q000235:genetics*; D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics* / Q000502:physiology; D029681:Arabidopsis Proteins / Q000235:genetics; D058639:Cold-Shock Response / Q000235:genetics*; D005615:Freezing; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D031286:Jatropha / Q000235:genetics*; D008969:Molecular Sequence Data; D010802:Phylogeny; D018515:Plant Leaves / Q000235:genetics / Q000502:physiology; D010940:Plant Proteins / Q000235:genetics*; D030821:Plants, Genetically Modified / Q000502:physiology; D036226:Seedlings / Q000235:genetics / Q000502:physiology; D015534:Trans-Activators / Q000235:genetics*; D019076:Transgenes
chemical_list: D029681:Arabidopsis Proteins; D010940:Plant Proteins; C520629:RD29a protein, Arabidopsis; D015534:Trans-Activators; C072392:COR6.6 protein, Arabidopsis