title: A dot-ELISA test using a Gnathostoma spinigerum recombinant matrix metalloproteinase protein for the serodiagnosis of human gnathostomiasis.
abstract: Gnathostomiasis caused by Gnathostoma spinigerum, is a hazardous food- borne helminthic zoonosis, and is endemic especially in developing countries in Asia. Definitive diagnosis, relying upon identification of worms from human tissues or body, is rarely accomplished. Consequently, sensitive supporting tools such as serological tests have been used widely. But these methods are time con- suming, need sophisticated equipment and are impractical in some settings. In the present study a dot enzyme-linked immunosorbent assay (dot-ELISA), using C. spin igerum recombinant matrix metalloproteinase protein as the antigen, was developed and assessed using sera of gnathostomiasis and other parasitosis pa- tients as well as healthy controls. The accuracy, sensitivity, specificity, positive and negative predictive values were 97.4%, 100%, 96.1%, 92.9%, and 100%, respectively. The dot-ELISA appears to be a suitable rapid test for diagnostic purpose as well as epidemiological studies.
authors.forename: Suphakdee
authors.initials: S
authors.lastname: Saenseeha
authors.forename: Janwan
authors.initials: J
authors.lastname: Penchom
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Yamasaki
authors.forename: Porntip
authors.initials: P
authors.lastname: Laummaunwai
authors.forename: Chatchai
authors.initials: C
authors.lastname: Tayapiwatana
authors.forename: Amnat
authors.initials: A
authors.lastname: Kitkhuandee
authors.forename: Wanchai
authors.initials: W
authors.lastname: Maleewong
authors.forename: Pewpan M
authors.initials: PM
authors.lastname: Intapan
mesh_terms: D000818:Animals; D004797:Enzyme-Linked Immunosorbent Assay; D058429:Gnathostomiasis / Q000175:diagnosis*; D006801:Humans; D020782:Matrix Metalloproteinases; D011994:Recombinant Proteins; D012680:Sensitivity and Specificity; D012698:Serologic Tests
chemical_list: D011994:Recombinant Proteins; D020782:Matrix Metalloproteinases