title: Combining in-cell NMR and X-ray fluorescence microscopy to reveal the intracellular maturation states of human superoxide dismutase 1.
abstract: An integrated approach which combines in-cell NMR spectroscopy with optical and X-ray fluorescence microscopy was developed to describe the intracellular maturation state of human Cu,Zn-SOD1. Microscopy data show a correlation between the intracellular levels of SOD1 and the content of zinc, corresponding to zinc binding to SOD1 observed by in-cell NMR.
authors.affiliation: Magnetic Resonance Center - CERM, University of Florence, Via Luigi Sacconi 6, 50019, Sesto Fiorentino, Florence, Italy. banci@cerm.unifi.it.
authors.forename: E
authors.initials: E
authors.lastname: Luchinat
authors.forename: A
authors.initials: A
authors.lastname: Gianoncelli
authors.forename: T
authors.initials: T
authors.lastname: Mello
authors.forename: A
authors.initials: A
authors.lastname: Galli
authors.forename: L
authors.initials: L
authors.lastname: Banci
mesh_terms: D003300:Copper / Q000737:chemistry; D005453:Fluorescence; D006801:Humans; D042541:Intracellular Space; D009682:Magnetic Resonance Spectroscopy; D013482:Superoxide Dismutase / Q000737:chemistry* / Q000378:metabolism / Q000648:ultrastructure; D000072105:Superoxide Dismutase-1; D014965:X-Rays; D015032:Zinc / Q000737:chemistry
chemical_list: C000606290:SOD1 protein, human; D003300:Copper; D013482:Superoxide Dismutase; D000072105:Superoxide Dismutase-1; D015032:Zinc