title: Application of high-throughput, high-resolution and cost-effective next generation sequencing-based large-scale HLA typing in donor registry.
abstract: Next generation sequencing (NGS)-based human leukocyte antigen (HLA) typing was used for ultra large-scale genotyping of registry donors for the China Marrow Donor Program (CMDP). More than 79,000 samples were subjected to HLA genotyping at 4-digit allelic level without ambiguities for HLA-A, -B, -C, DRB1 and DQB1 loci, with throughput up to 2068 samples per lane in a HiSeq flow cell (eight lanes per run), and cost reduced by 95% compared with that of Sanger-based typing. Two percent of randomly selected samples were quality control (QC) tested at 4-digit allelic level by the CMDP QC laboratory, yielded a concordance of 99.72%. These results demonstrate that NGS is a cost effective and valuable tool for HLA typing of registry donors.
authors.affiliation: BGI Diagnosis Co., Ltd, Shenzhen, China.
authors.forename: M
authors.initials: M
authors.lastname: Zhou
authors.forename: D
authors.initials: D
authors.lastname: Gao
authors.forename: X
authors.initials: X
authors.lastname: Chai
authors.forename: J
authors.initials: J
authors.lastname: Liu
authors.forename: Z
authors.initials: Z
authors.lastname: Lan
authors.forename: Q
authors.initials: Q
authors.lastname: Liu
authors.forename: F
authors.initials: F
authors.lastname: Yang
authors.forename: Y
authors.initials: Y
authors.lastname: Guo
authors.forename: J
authors.initials: J
authors.lastname: Fang
authors.forename: L
authors.initials: L
authors.lastname: Yang
authors.forename: D
authors.initials: D
authors.lastname: Du
authors.forename: L
authors.initials: L
authors.lastname: Chen
authors.forename: X
authors.initials: X
authors.lastname: Yang
authors.forename: M
authors.initials: M
authors.lastname: Zhang
authors.forename: H
authors.initials: H
authors.lastname: Zeng
authors.forename: J
authors.initials: J
authors.lastname: Lu
authors.forename: H
authors.initials: H
authors.lastname: Chen
authors.forename: X
authors.initials: X
authors.lastname: Zhang
authors.forename: S
authors.initials: S
authors.lastname: Wu
authors.forename: Y
authors.initials: Y
authors.lastname: Han
authors.forename: J
authors.initials: J
authors.lastname: Tan
authors.forename: Z
authors.initials: Z
authors.lastname: Cheng
authors.forename: C
authors.initials: C
authors.lastname: Huang
authors.forename: W
authors.initials: W
authors.lastname: Wang
mesh_terms: D016026:Bone Marrow Transplantation; D006680:HLA Antigens / Q000145:classification* / Q000235:genetics / Q000276:immunology; D059014:High-Throughput Nucleotide Sequencing / Q000191:economics* / Q000295:instrumentation / Q000379:methods; D006650:Histocompatibility Testing; D006801:Humans; D011786:Quality Control; D012042:Registries; D014019:Tissue Donors
chemical_list: D006680:HLA Antigens
keywords: allelic level; cost effective; genotyping; high throughput; human leukocyte antigen; next generation sequencing