title: Chromatographic purification of adenoviral vectors on anion-exchange resins.
abstract: Anion-exchange chromatography is a useful and effective tool for adenoviral vectors purification. However, due to the different functional groups and matrices, both binding capacity and resolution of most AEC resins are different. In this study, four different AEC resins are compared by the binding capacity, resolution and recovery. Using Fractogel TMAE as an adsorbent to purify adenoviral vectors has obvious advantages over the other resins, namely (1) dynamic binding capacity is higher than other resins; (2) unprecedented sharpness (1,570,000±250,000) and symmetry of adenoviral vectors peak (1.67±0.06); (3) higher resolution with other contaminants (2.16±0.08); (4) no enzymatic treatment; (5) the recovery can reach 75%; (6) the purity is higher and the total virion to infectious particle ratios can reach 18.9. In the present work, we confirmed the possibility of purifying pharmaceutical-grade adenoviral vectors by AEC.
authors.affiliation: School of Bioscience and Biopharmaceutics, Guangdong Province Key Laboratory for Biotechnology Drug Candidates, Guangdong Pharmaceutical University, 510006 Guangzhou, Guangdong, PR China.
authors.forename: Huaben
authors.initials: H
authors.lastname: Bo
authors.affiliation: College of Pharmacy, Guangdong Pharmaceutical University, 510006 Guangzhou, Guangdong, PR China.
authors.forename: Jun
authors.initials: J
authors.lastname: Chen
authors.affiliation: School of Bioscience and Biopharmaceutics, Guangdong Province Key Laboratory for Biotechnology Drug Candidates, Guangdong Pharmaceutical University, 510006 Guangzhou, Guangdong, PR China.
authors.forename: Ting
authors.initials: T
authors.lastname: Liang
authors.affiliation: School of Bioscience and Biopharmaceutics, Guangdong Province Key Laboratory for Biotechnology Drug Candidates, Guangdong Pharmaceutical University, 510006 Guangzhou, Guangdong, PR China.
authors.forename: Senhai
authors.initials: S
authors.lastname: Li
authors.affiliation: School of Bioscience and Biopharmaceutics, Guangdong Province Key Laboratory for Biotechnology Drug Candidates, Guangdong Pharmaceutical University, 510006 Guangzhou, Guangdong, PR China.
authors.forename: Hongwei
authors.initials: H
authors.lastname: Shao
authors.affiliation: School of Bioscience and Biopharmaceutics, Guangdong Province Key Laboratory for Biotechnology Drug Candidates, Guangdong Pharmaceutical University, 510006 Guangzhou, Guangdong, PR China. Electronic address: shulhuang@sina.com.
authors.forename: Shulin
authors.initials: S
authors.lastname: Huang
mesh_terms: D000256:Adenoviridae / Q000235:genetics / Q000302:isolation & purification*; D000837:Anion Exchange Resins; D002852:Chromatography, Ion Exchange; D005822:Genetic Vectors / Q000235:genetics / Q000302:isolation & purification*; D049452:Green Fluorescent Proteins / Q000235:genetics; D057809:HEK293 Cells; D006801:Humans
chemical_list: D000837:Anion Exchange Resins; D049452:Green Fluorescent Proteins
keywords: Adenoviral vector; Anion-exchange chromatography; Gene therapy; Vaccines