title: Identification of clinical aeromonas species by rpoB and gyrB sequencing and development of a multiplex PCR method for detection of Aeromonas hydrophila, A. caviae, A. veronii, and A. media.
abstract: Conventional identification of Aeromonas species based on biochemical methods is challenged by the heterogeneous nature of the species. Here, we present a new multiplex PCR method directed toward the gyrB and rpoB genes that identifies four Aeromonas species, A. hydrophila, A. media, A. veronii, and A. caviae, and we describe the application of this method on a Danish strain collection.
authors.affiliation: Statens Serum Institut, Department of Microbiology and Infection Control, Copenhagen, Denmark spn@ssi.dk.
authors.forename: Søren
authors.initials: S
authors.lastname: Persson
authors.affiliation: Statens Serum Institut, Department of Microbiology and Infection Control, Copenhagen, Denmark.
authors.forename: Suzan
authors.initials: S
authors.lastname: Al-Shuweli
authors.affiliation: Statens Serum Institut, Department of Microbiology and Infection Control, Copenhagen, Denmark.
authors.forename: Seval
authors.initials: S
authors.lastname: Yapici
authors.affiliation: Statens Serum Institut, Department of Microbiology and Infection Control, Copenhagen, Denmark.
authors.forename: Joan N
authors.initials: JN
authors.lastname: Jensen
authors.affiliation: Statens Serum Institut, Department of Microbiology and Infection Control, Copenhagen, Denmark.
authors.forename: Katharina E P
authors.initials: KE
authors.lastname: Olsen
mesh_terms: D000333:Aeromonas / Q000145:classification* / Q000302:isolation & purification*; D001431:Bacteriological Techniques / Q000379:methods*; D027081:DNA Gyrase / Q000235:genetics; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D012321:DNA-Directed RNA Polymerases / Q000235:genetics; D003718:Denmark; D016905:Gram-Negative Bacterial Infections / Q000175:diagnosis* / Q000382:microbiology; D006801:Humans; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D008969:Molecular Sequence Data; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods*; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D012321:DNA-Directed RNA Polymerases; C105078:RNA polymerase beta subunit; D027081:DNA Gyrase