title: Hydroxybutyl Chitosan Polymer-Mediated CD133 Antibody Coating of Metallic Stents to Reduce Restenosis in a Porcine Model of Atherosclerosis.
abstract: Antibody-coated stents to capture circulating endothelial progenitor cells (EPCs) for re-endothelialization appear to be a novel therapeutic option for the treatment of atherosclerotic disease. Hydroxybutyl chitosan (HBC), a linear polysaccharide made from shrimps and other crustacean shells, is biocompatible, nontoxic, and hydrophilic, making it ideal for biomedical applications. In this study, HBC was explored for the immobilization of anti-CD133 antibodies. We demonstrated that CD133 antibodies mediated by HBC were successfully coated on cobalt-chromium alloy discs and metal stents. The coating was homogeneous and smooth as shown by electronic microscopy analysis. Balloon expansion of coated stents did not cause cracking or peeling. The HBC discs promoted CD133+ EPCs and human umbilical vein endothelial cell growth in vitro. The CD133 antibody-coated but not bare discs bound CD133+ EPCs in vitro. Implantation of CD133 antibody-coated stents significantly inhibited intimal hyperplasia and reduced restenosis compared with implantation of bare stents in a porcine model of atherosclerosis. These findings suggest HBC is a valuable anchoring agent that can be applied for bioactive coating of stents and that CD133 antibody-coated stents might be a potential therapeutic alternative for the treatment of atherosclerotic disease.
authors.affiliation: Department of Cardiology, The Affiliated Hospital of Medical College, Qingdao University, Qingdao, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Li
authors.affiliation: Division of Cardiology, Keenan Research Center for Biomedical Science at the Li Ka Shing Knowledge Institute, St. Michael's Hospital, University of Toronto, Toronto, Canada.
authors.forename: Qiuwang
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: Department of Cardiology, The Affiliated Hospital of Medical College, Qingdao University, Qingdao, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Li
authors.affiliation: Department of Cardiology, The Affiliated Hospital of Medical College, Qingdao University, Qingdao, China dryian2014@gmail.com.
authors.forename: Yi
authors.initials: Y
authors.lastname: An
authors.affiliation: Division of Cardiology, Keenan Research Center for Biomedical Science at the Li Ka Shing Knowledge Institute, St. Michael's Hospital, University of Toronto, Toronto, Canada.
authors.forename: Michael B J
authors.initials: MB
authors.lastname: Kutryk
mesh_terms: D000071916:AC133 Antigen; D000818:Animals; D055910:Antibodies, Immobilized / Q000008:administration & dosage*; D015703:Antigens, CD / Q000276:immunology*; D050197:Atherosclerosis / Q000628:therapy*; D049109:Cell Proliferation; D002478:Cells, Cultured; D048271:Chitosan / Q000008:administration & dosage*; D066026:Endothelial Progenitor Cells / Q000502:physiology*; D006023:Glycoproteins / Q000276:immunology*; D006801:Humans; D006965:Hyperplasia; D008297:Male; D058426:Neointima / Q000473:pathology; D010455:Peptides / Q000276:immunology*; D011108:Polymers / Q000008:administration & dosage*; D015607:Stents; D013552:Swine
chemical_list: D000071916:AC133 Antigen; D055910:Antibodies, Immobilized; D015703:Antigens, CD; D006023:Glycoproteins; C000606023:PROM1 protein, human; D010455:Peptides; D011108:Polymers; D048271:Chitosan
keywords: CD133 antibody; endothelial progenitor cells; hydroxybutyl chitosan; re-endothelialization; restenosis; stent coating