title: Pineapple [Ananas comosus (L.) Merr].
abstract: The efficacy of Agrobacterium-mediated pineapple transformation technique has been improved (mean percentage of transgenic micro-shoots regenerated from initial callus explants up to 20.6%) using a novel encapsulation-based, antibiotic selection procedure. The detailed protocol using a standard plant transformation vector (pCAMBIA1304) as reported in an 'elite' Indian variety (Queen) of pineapple [Ananas comosus (L.) Merr] can be applied to other varieties of pineapple for introgression of target genes.
authors.affiliation: Division of Plant Biology, Bose Institute, 93/1 APC Road, Kolkata, 700 009, India, gaurab@jcbose.ac.in.
authors.forename: Gaurab
authors.initials: G
authors.lastname: Gangopadhyay
authors.forename: Kalyan K
authors.initials: KK
authors.lastname: Mukherjee
mesh_terms: D016960:Agrobacterium tumefaciens / Q000235:genetics / Q000254:growth & development; D000464:Alginates / Q000737:chemistry; D031230:Ananas / Q000235:genetics* / Q000254:growth & development*; D018920:Coculture Techniques; D005818:Genetic Engineering / Q000379:methods*; D020723:Glucuronic Acid / Q000737:chemistry; D005966:Glucuronidase / Q000235:genetics; D006603:Hexuronic Acids / Q000737:chemistry; D008863:Microspheres; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction; D013242:Sterilization; D014170:Transformation, Genetic
chemical_list: D000464:Alginates; D006603:Hexuronic Acids; D020723:Glucuronic Acid; D005966:Glucuronidase