title: Neural-competent cells of adult human dermis belong to the Schwann lineage.
abstract: Resident neural precursor cells (NPCs) have been reported for a number of adult tissues. Understanding their physiological function or, alternatively, their activation after tissue damage or in vitro manipulation remains an unsolved issue. Here, we investigated the source of human dermal NPCs in adult tissue. By following an unbiased, comprehensive approach employing cell-surface marker screening, cell separation, transcriptomic characterization, and in vivo fate analyses, we found that p75NTR(+) precursors of human foreskin can be ascribed to the Schwann (CD56(+)) and perivascular (CD56(-)) cell lineages. Moreover, neural differentiation potential was restricted to the p75NTR(+)CD56(+) Schwann cells and mediated by SOX2 expression levels. Double-positive NPCs were similarly obtained from human cardiospheres, indicating that this phenomenon might be widespread.
authors.affiliation: Tissue Engineering Laboratory, Bioengineering Area, Instituto Biodonostia, Hospital Universitario Donostia, Paseo Dr. Begiristain s/n, 20014 San Sebastián, Spain.
authors.forename: Usue
authors.initials: U
authors.lastname: Etxaniz
authors.affiliation: Tissue Engineering Laboratory, Bioengineering Area, Instituto Biodonostia, Hospital Universitario Donostia, Paseo Dr. Begiristain s/n, 20014 San Sebastián, Spain.
authors.forename: Adrián
authors.initials: A
authors.lastname: Pérez-San Vicente
authors.affiliation: Tissue Engineering Laboratory, Bioengineering Area, Instituto Biodonostia, Hospital Universitario Donostia, Paseo Dr. Begiristain s/n, 20014 San Sebastián, Spain; Division of Cardiology, University of Washington Medical Center, 850 Republican Street, Seattle, WA 98195, USA.
authors.forename: Nuria
authors.initials: N
authors.lastname: Gago-López
authors.affiliation: Cell Differentiation Lab, Stem Cells Department, Centro Andaluz de Biología Molecular y Medicina Regenerativa (CABIMER), Avenida Americo Vespucio s/n, 41092 Sevilla, Spain.
authors.forename: Mario
authors.initials: M
authors.lastname: García-Dominguez
authors.affiliation: Tissue Engineering Laboratory, Bioengineering Area, Instituto Biodonostia, Hospital Universitario Donostia, Paseo Dr. Begiristain s/n, 20014 San Sebastián, Spain.
authors.forename: Haizea
authors.initials: H
authors.lastname: Iribar
authors.affiliation: Cell Cytometry and Microscopy Unit, Fundación Inbiomed, Paseo Mikeletegi 81, 20009 San Sebastián, Spain.
authors.forename: Ariane
authors.initials: A
authors.lastname: Aduriz
authors.affiliation: Tissue Engineering Laboratory, Bioengineering Area, Instituto Biodonostia, Hospital Universitario Donostia, Paseo Dr. Begiristain s/n, 20014 San Sebastián, Spain.
authors.forename: Virginia
authors.initials: V
authors.lastname: Pérez-López
authors.affiliation: Tissue Engineering Laboratory, Bioengineering Area, Instituto Biodonostia, Hospital Universitario Donostia, Paseo Dr. Begiristain s/n, 20014 San Sebastián, Spain.
authors.forename: Izaskun
authors.initials: I
authors.lastname: Burgoa
authors.affiliation: Neuroscience Area, Instituto Biodonostia, Hospital Universitario Donostia, Paseo Doctor Begiristain s/n, 20014 San Sebastián, Spain.
authors.forename: Haritz
authors.initials: H
authors.lastname: Irizar
authors.affiliation: Neuroscience Area, Instituto Biodonostia, Hospital Universitario Donostia, Paseo Doctor Begiristain s/n, 20014 San Sebastián, Spain.
authors.forename: Maider
authors.initials: M
authors.lastname: Muñoz-Culla
authors.affiliation: Neuroscience Area, Instituto Biodonostia, Hospital Universitario Donostia, Paseo Doctor Begiristain s/n, 20014 San Sebastián, Spain.
authors.forename: Ainara
authors.initials: A
authors.lastname: Vallejo-Illarramendi
authors.affiliation: StemTek Therapeutics SL, Rodriguez Arias 23, 48011 Bilbao, Spain.
authors.forename: Olatz
authors.initials: O
authors.lastname: Leis
authors.affiliation: Oncology Area, Instituto Biodonostia, Hospital Universitario Donostia, Paseo Doctor Begiristain s/n, 20014 San Sebastián, Spain.
authors.forename: Ander
authors.initials: A
authors.lastname: Matheu
authors.affiliation: StemTek Therapeutics SL, Rodriguez Arias 23, 48011 Bilbao, Spain.
authors.forename: Angel G
authors.initials: AG
authors.lastname: Martín
authors.affiliation: Neuroscience Area, Instituto Biodonostia, Hospital Universitario Donostia, Paseo Doctor Begiristain s/n, 20014 San Sebastián, Spain.
authors.forename: David
authors.initials: D
authors.lastname: Otaegui
authors.affiliation: Cell Cytometry and Microscopy Unit, Fundación Inbiomed, Paseo Mikeletegi 81, 20009 San Sebastián, Spain.
authors.forename: María Paz
authors.initials: MP
authors.lastname: López-Mato
authors.affiliation: Tissue Engineering Laboratory, Bioengineering Area, Instituto Biodonostia, Hospital Universitario Donostia, Paseo Dr. Begiristain s/n, 20014 San Sebastián, Spain.
authors.forename: Araika
authors.initials: A
authors.lastname: Gutiérrez-Rivera
authors.affiliation: Division of Cardiology, University of Washington Medical Center, 850 Republican Street, Seattle, WA 98195, USA.
authors.forename: Robb
authors.initials: R
authors.lastname: MacLellan
authors.affiliation: Tissue Engineering Laboratory, Bioengineering Area, Instituto Biodonostia, Hospital Universitario Donostia, Paseo Dr. Begiristain s/n, 20014 San Sebastián, Spain. Electronic address: ander.izeta@biodonostia.org.
authors.forename: Ander
authors.initials: A
authors.lastname: Izeta
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000818:Animals; D019002:CD56 Antigen / Q000235:genetics / Q000378:metabolism; D002454:Cell Differentiation / Q000235:genetics; D019070:Cell Lineage; D002478:Cells, Cultured; D002648:Child; D002675:Child, Preschool; D020405:Dermis / Q000166:cytology* / Q000378:metabolism; D052816:Foreskin / Q000166:cytology; D020869:Gene Expression Profiling; D006801:Humans; D007223:Infant; D008297:Male; D051379:Mice; D018613:Microscopy, Confocal; D008875:Middle Aged; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism; D058953:Neural Stem Cells / Q000166:cytology* / Q000378:metabolism; D009474:Neurons / Q000166:cytology / Q000378:metabolism; D017475:Receptors, Nerve Growth Factor / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012583:Schwann Cells / Q000166:cytology* / Q000378:metabolism; D055815:Young Adult
chemical_list: D019002:CD56 Antigen; C484065:NGFR protein, human; D009419:Nerve Tissue Proteins; D017475:Receptors, Nerve Growth Factor