title: A phthalimidation protocol that follows protein defined parameters.
abstract: This work outlines the first phthalimidation protocol suitable for protein labeling and performed in aqueous media at room temperature and neutral pH with no catalyst or co-reagent required. The methodology is suitable for a range of amines and its efficiency was determined with chemoselective and site-selective protein labeling.
authors.affiliation: Department of Chemistry, Indian Institute of Science Education and Research (IISER) Bhopal, Indore By-pass Road, Bhauri, Bhopal, MP, India 462066. vrai@iiserb.ac.in.
authors.forename: Rohith
authors.initials: R
authors.lastname: Singudas
authors.forename: Srinivasa Rao
authors.initials: SR
authors.lastname: Adusumalli
authors.forename: Pralhad Namdev
authors.initials: PN
authors.lastname: Joshi
authors.forename: Vishal
authors.initials: V
authors.lastname: Rai
mesh_terms: D000588:Amines / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D010797:Phthalimides / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry*; D013194:Staining and Labeling / Q000379:methods*; D013696:Temperature; D014867:Water / Q000737:chemistry*
chemical_list: D000588:Amines; D010797:Phthalimides; D011506:Proteins; D014867:Water