title: Induction, purification and characterization of a novel manganese peroxidase from Irpex lacteus CD2 and its application in the decolorization of different types of dye.
abstract: Manganese peroxidase (MnP) is the one of the important ligninolytic enzymes produced by lignin-degrading fungi which has the great application value in the field of environmental biotechnology. Searching for new MnP with stronger tolerance to metal ions and organic solvents is important for the maximization of potential of MnP in the biodegradation of recalcitrant xenobiotics. In this study, it was found that oxalic acid, veratryl alcohol and 2,6-Dimehoxyphenol could stimulate the synthesis of MnP in the white-rot fungus Irpex lacteus CD2. A novel manganese peroxidase named as CD2-MnP was purified and characterized from this fungus. CD2-MnP had a strong capability for tolerating different metal ions such as Ca2+, Cd2+, Co2+, Mg2+, Ni2+ and Zn2+ as well as organic solvents such as methanol, ethanol, DMSO, ethylene glycol, isopropyl alcohol, butanediol and glycerin. The different types of dyes including the azo dye (Remazol Brilliant Violet 5R, Direct Red 5B), anthraquinone dye (Remazol Brilliant Blue R), indigo dye (Indigo Carmine) and triphenylmethane dye (Methyl Green) as well as simulated textile wastewater could be efficiently decolorized by CD2-MnP. CD2-MnP also had a strong ability of decolorizing different dyes with the coexistence of metal ions and organic solvents. In summary, CD2-MnP from Irpex lacteus CD2 could effectively degrade a broad range of synthetic dyes and exhibit a great potential for environmental biotechnology.
authors.affiliation: College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, 430074, China.
authors.forename: Xing
authors.initials: X
authors.lastname: Qin
authors.affiliation: College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, 430074, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Zhang
authors.affiliation: College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, 430074, China.
authors.forename: Xiaoyu
authors.initials: X
authors.lastname: Zhang
authors.affiliation: College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, 430074, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Yang
mesh_terms: D000880:Anthraquinones / Q000378:metabolism; D001391:Azo Compounds / Q000378:metabolism; D001487:Basidiomycota / Q000187:drug effects / Q000201:enzymology*; D001592:Benzyl Alcohols / Q000494:pharmacology; D001673:Biodegradation, Environmental; D004396:Coloring Agents / Q000378:metabolism*; D005656:Fungal Proteins / Q000302:isolation & purification* / Q000378:metabolism; D007203:Indigo Carmine / Q000378:metabolism; D008739:Methyl Green / Q000378:metabolism; D019815:Oxalic Acid / Q000494:pharmacology; D010544:Peroxidases / Q000302:isolation & purification* / Q000378:metabolism
chemical_list: D000880:Anthraquinones; D001391:Azo Compounds; D001592:Benzyl Alcohols; D004396:Coloring Agents; D005656:Fungal Proteins; D008739:Methyl Green; D019815:Oxalic Acid; D007203:Indigo Carmine; D010544:Peroxidases; C051129:manganese peroxidase; C024758:Remazol Brilliant Blue R; C042197:veratryl alcohol