title: Microbial diversity in sugarcane ethanol production in a Brazilian distillery using a culture-independent method.
abstract: Sugarcane ethanol production occurs in non-sterile conditions, and microbial contamination can decrease productivity. In this study, we assessed the microbial diversity of contaminants of ethanol production in an industrial facility in Brazil. Samples obtained at different stages were analyzed by pyrosequencing-based profiling of bacterial and archaeal 16S rRNA genes and the fungal internal transcribed spacer region. A total of 355 bacterial groups, 22 archaeal groups, and 203 fungal groups were identified, and community changes were related to temperature changes at certain stages. After fermentation, Lactobacillus and unclassified Lactobacillaceae accounted for nearly 100 % of the bacterial sequences. Predominant Fungi groups were "unclassified Fungi," Meyerozyma, and Candida. The predominant Archaea group was unclassified Thaumarchaeota. This is the first work to assess the diversity of Bacteria, and Archaea and Fungi associated with the industrial process of sugarcane-ethanol production using culture-independent techniques.
authors.affiliation: Genomic Sciences and Biotechnology Program, Universidade Católica de Brasília, Brasília, DF, 70790-160, Brazil.
authors.forename: Ohana Yonara Assis
authors.initials: OY
authors.lastname: Costa
authors.forename: Betulia Morais
authors.initials: BM
authors.lastname: Souto
authors.forename: Daiva Domenech
authors.initials: DD
authors.lastname: Tupinambá
authors.forename: Jessica Carvalho
authors.initials: JC
authors.lastname: Bergmann
authors.forename: Cynthia Maria
authors.initials: CM
authors.lastname: Kyaw
authors.forename: Ricardo Henrique
authors.initials: RH
authors.lastname: Kruger
authors.forename: Cristine Chaves
authors.initials: CC
authors.lastname: Barreto
authors.forename: Betania Ferraz
authors.initials: BF
authors.lastname: Quirino
mesh_terms: D001105:Archaea / Q000145:classification* / Q000302:isolation & purification / Q000378:metabolism; D001419:Bacteria / Q000145:classification* / Q000302:isolation & purification / Q000378:metabolism; D044822:Biodiversity; D056804:Biofuels; D001938:Brazil; D003470:Culture Media / Q000737:chemistry; D046508:Culture Techniques; D019641:DNA, Archaeal / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D004271:DNA, Fungal / Q000235:genetics; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation; D005658:Fungi / Q000145:classification* / Q000302:isolation & purification / Q000378:metabolism; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D031786:Saccharum / Q000382:microbiology*; D017422:Sequence Analysis, DNA
chemical_list: D056804:Biofuels; D003470:Culture Media; D019641:DNA, Archaeal; D004269:DNA, Bacterial; D004271:DNA, Fungal; D012336:RNA, Ribosomal, 16S; D000431:Ethanol