title: Physical mapping of the 5S and 18S rDNA in ten species of Hypostomus Lacépède 1803 (Siluriformes: Loricariidae): evolutionary tendencies in the genus.
abstract: Hypostomus is a diverse group with unclear aspects regarding its biology, including the mechanisms that led to chromosome diversification within the group. Fluorescence in situ hybridization (FISH) with 5S and 18S rDNA probes was performed on ten Hypostomini species. Hypostomus faveolus, H. cochliodon, H. albopunctatus, H. aff. paulinus, and H. topavae had only one chromosome pair with 18S rDNA sites, while H. ancistroides, H. commersoni, H. hermanni, H. regani, and H. strigaticeps had multiple 18S rDNA sites. Regarding the 5S rDNA genes, H. ancistroides, H. regani, H. albopunctatus, H. aff. paulinus, and H. topavae had 5S rDNA sites on only one chromosome pair and H. faveolus, H. cochliodon, H. commersoni, H. hermanni, and H. strigaticeps had multiple 5S rDNA sites. Most species had 18S rDNA sites in the telomeric region of the chromosomes. All species but H. cochliodon had 5S rDNA in the centromeric/pericentromeric region of one metacentric pair. Obtained results are discussed based on existent phylogenies for the genus, with comments on possible dispersion mechanisms to justify the variability of the rDNA sites in Hypostomus.
authors.affiliation: Universidade Tecnológica Federal do Paraná, Prolongamento da Rua Cerejeira s/n, São Luiz, 85892-00l Santa Helena, PR, Brazil.
authors.forename: Vanessa
authors.identifier: 0000-0003-0508-1666
authors.initials: V
authors.lastname: Bueno
authors.affiliation: Departamento de Biologia e Zoologia, Universidade Federal de Mato Grosso, Instituto de Biociências, Avenida Fernando Correa da Costa s/n, Coxipó, 78060-900 Cuiabá, MT, Brazil.
authors.forename: Paulo César
authors.initials: PC
authors.lastname: Venere
authors.affiliation: Universidade Estadual do Oeste do Paraná, Centro de Ciências Biológicas e da Saúde, Rua Universitária 2069, 85801-110 Cascavel, PR, Brazil.
authors.forename: Jocicléia
authors.initials: J
authors.lastname: Thums Konerat
authors.affiliation: Departamento de Biologia/Nupélia, Universidade Estadual de Maringá, Avenida Colombo 5790, 87020-900 Maringá, PR, Brazil.
authors.forename: Cláudio Henrique
authors.initials: CH
authors.lastname: Zawadzki
authors.affiliation: Departamento de Biologia Estrutural, Molecular e Genética, Universidade Estadual de Ponta Grossa, Avenida Carlos Cavalcanti 4748, 84030-900 Ponta Grossa, PR, Brazil.
authors.forename: Marcelo Ricardo
authors.initials: MR
authors.lastname: Vicari
authors.affiliation: Universidade Estadual do Oeste do Paraná, Centro de Ciências Biológicas e da Saúde, Rua Universitária 2069, 85801-110 Cascavel, PR, Brazil.
authors.forename: Vladimir Pavan
authors.initials: VP
authors.lastname: Margarido
mesh_terms: D000818:Animals; D005075:Biological Evolution; D001938:Brazil; D002397:Catfishes / Q000145:classification / Q000235:genetics*; D002503:Centromere / Q000737:chemistry; D029941:Fish Proteins / Q000235:genetics*; D020459:Genes, rRNA; D059785:Karyotype; D010802:Phylogeny; D058974:Phylogeography; D020161:Physical Chromosome Mapping; D011003:Ploidies; D012337:RNA, Ribosomal, 18S / Q000235:genetics*; D012341:RNA, Ribosomal, 5S / Q000235:genetics*; D016615:Telomere / Q000737:chemistry
chemical_list: D029941:Fish Proteins; D012337:RNA, Ribosomal, 18S; D012341:RNA, Ribosomal, 5S