title: Precise and long-term tracking of adipose-derived stem cells and their regenerative capacity via superb bright and stable organic nanodots.
abstract: Monitoring and understanding long-term fate and regenerative therapy of administrated stem cells in vivo is of great importance. Herein we report organic nanodots with aggregation-induced emission characteristics (AIE dots) for long-term tracking of adipose-derived stem cells (ADSCs) and their regenerative capacity in living mice. The AIE dots possess high fluorescence (with a high quantum yield of 25±1%), excellent biological and photophysical stabilities, low in vivo toxicity, and superb retention in living ADSCs with negligible interference on their pluripotency and secretome. These AIE dots also exhibit superior in vitro cell tracking capability compared to the most popular commercial cell trackers, PKH26 and Qtracker 655. In vivo quantitative studies with bioluminescence and GFP labeling as the controls reveal that the AIE dots can precisely and quantitatively report the fate of ADSCs and their regenerative capacity for 42 days in an ischemic hind limb bearing mouse model.
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology, Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Sciences, and Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Nankai University , Tianjin 300071, P. R. China.
authors.forename: Dan
authors.initials: D
authors.lastname: Ding
authors.forename: Duo
authors.initials: D
authors.lastname: Mao
authors.forename: Kai
authors.initials: K
authors.lastname: Li
authors.forename: Xiaomin
authors.initials: X
authors.lastname: Wang
authors.forename: Wei
authors.initials: W
authors.lastname: Qin
authors.forename: Rongrong
authors.initials: R
authors.lastname: Liu
authors.forename: David Shunzhong
authors.initials: DS
authors.lastname: Chiam
authors.forename: Nikodem
authors.initials: N
authors.lastname: Tomczak
authors.forename: Zhimou
authors.initials: Z
authors.lastname: Yang
authors.forename: Ben Zhong
authors.initials: BZ
authors.lastname: Tang
authors.forename: Deling
authors.initials: D
authors.lastname: Kong
authors.forename: Bin
authors.initials: B
authors.lastname: Liu
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D058948:Cell Tracking / Q000379:methods*; D006614:Hindlimb / Q000098:blood supply / Q000502:physiology; D007511:Ischemia / Q000628:therapy; D008297:Male; D051379:Mice; D008958:Models, Molecular; D008968:Molecular Conformation; D053758:Nanoparticles; D009930:Organic Chemicals / Q000737:chemistry*; D012038:Regeneration; D033581:Stem Cell Transplantation; D013234:Stem Cells / Q000166:cytology*
chemical_list: D009930:Organic Chemicals
keywords: adipose-derived stem cells; aggregation-induced emission; organic nanodots; regenerative capacity; stem cell tracking