title: Quantitative DNA methylation analysis for epigenotyping of colorectal cancer.
abstract: Accumulation of epigenetic alteration plays important roles in tumorigenesis. Aberrant DNA hypermethylation in gene promoter regions is a common epigenetic mechanism for silencing tumor suppressor genes in many types of cancer including colorectal cancer (CRC). By using quantitative methylation information, CRC can be classified into three distinct methylation epigenotypes with different genetic features, suggesting existence of at least three molecular pathways in genesis of CRC. We describe in this chapter, the methods for analyses of aberrant DNA methylation to epigenotype CRC.
authors.affiliation: Department of Molecular Oncology, Graduate School of Medicine, Chiba University, Inohana 1-8-1, Chuo-ku, Chiba, Japan, kaneda@chiba-u.jp.
authors.forename: Atsushi
authors.initials: A
authors.lastname: Kaneda
authors.forename: Koichi
authors.initials: K
authors.lastname: Yagi
mesh_terms: D016000:Cluster Analysis; D015179:Colorectal Neoplasms / Q000235:genetics*; D019175:DNA Methylation; D057890:Epigenomics / Q000379:methods*; D060005:Genotyping Techniques / Q000379:methods*; D006801:Humans; D009154:Mutation; D016133:Polymerase Chain Reaction; D012259:Ribonuclease, Pancreatic / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013447:Sulfites / Q000494:pharmacology; D014158:Transcription, Genetic
chemical_list: D013447:Sulfites; D012259:Ribonuclease, Pancreatic; C042345:hydrogen sulfite