title: [Correlation between five RNA markers of rat's skin and PMI at different temperatures].
abstract: OBJECTIVE
  To explore the correlation between postmortem interval (PMI) and five RNA markers of rat's skin--β-actin, glyceraldehyde-3-phosphate dehydrogenase (GAPDH), 18S ribosomal RNA(18S rRNA), 5S ribosomal RNA (5S rRNA), and microRNA-203 (miR-203), at different temperatures.
  
  
  METHODS
  Eighteen SD rats were randomly divided into three environmental temperature groups: 4 °C, 15 °C and 35 °C, respectively. Skin samples were taken at 11 time points from 0 h to 120 h post-mortem. The total RNA was extracted from the skin samples and the five RNA levels were detected by real-time fluorescent quantitative PCR. Proper internal reference was selected by geNorm software. Regression analysis of the RNA markers was conducted by GraphPad software.
  
  
  RESULTS
  5S rRNA and miR-203 were most suitable internal references. A good linear relationship between PMI and RNA levels (β-actin and GAPDH) was observed in two groups (4 °C and 15 °C), whereas the S type curve relationship between the expression levels of the two markers (β-actin and GAPDH) and PMI was observed in the 35 °C group. The partial linear relationship between 18S rRNA and PMI was observed in the groups (15 °C and 35 °C).
  
  
  CONCLUSIONS
  Skin could be a suitable material for extracting RNA. The RNA expression levels of β-actin and GAPDH correlate well with PMI, and these RNA markers of skin tissue could be additional indice for the estimation of PMI.
authors.forename: Hui
authors.initials: H
authors.lastname: Pan
authors.forename: Heng
authors.initials: H
authors.lastname: Zhang
authors.forename: Ye-hui
authors.initials: YH
authors.lastname: Lü
authors.forename: Jian-long
authors.initials: JL
authors.lastname: Ma
authors.forename: Kai-jun
authors.initials: KJ
authors.lastname: Ma
authors.forename: Long
authors.initials: L
authors.lastname: Chen
mesh_terms: D000199:Actins; D000818:Animals; D001344:Autopsy; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000235:genetics*; D011180:Postmortem Changes; D012313:RNA; D020871:RNA Stability; D012337:RNA, Ribosomal, 18S; D051381:Rats; D060888:Real-Time Polymerase Chain Reaction; D012044:Regression Analysis; D012867:Skin; D013696:Temperature
chemical_list: D000199:Actins; D012337:RNA, Ribosomal, 18S; D012313:RNA; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases