title: Polymer enzyme conjugates as chiral ligands for sharpless dihydroxylation of alkenes in organic solvents.
abstract: Conjugates of enzymes and poly(2-methyloxazoline) were used as organosoluble amphiphilic polymer nanocontainers for dissolving osmate, thereby converting the enzymes into organosoluble artificial metalloenzymes. These were shown to catalyze the dihydroxylation of different alkenes with high enantioselectivity. The highest selectivities, found for osmate complexed with laccase polymer-enzyme conjugates (PECs), even exceed those of classical Sharpless catalysts.
authors.affiliation: Chair of Biomaterials and Polymer Science, Department of Biochemical and Chemical Engineering, TU Dortmund, Emil-Figge-Strasse 66, 44227 Dortmund (Germany).
authors.forename: Stefan
authors.initials: S
authors.lastname: Konieczny
authors.forename: Melanie
authors.initials: M
authors.lastname: Leurs
authors.forename: Joerg C
authors.initials: JC
authors.lastname: Tiller
mesh_terms: D000475:Alkenes / Q000737:chemistry*; D055162:Biocatalysis; D002918:Chymotrypsin / Q000737:chemistry*; D005656:Fungal Proteins / Q000737:chemistry*; D006900:Hydroxylation; D042845:Laccase / Q000737:chemistry; D008024:Ligands; D008049:Lipase / Q000737:chemistry; D009113:Muramidase / Q000737:chemistry; D010080:Oxazoles / Q000737:chemistry; D009195:Peroxidase / Q000737:chemistry; D011108:Polymers / Q000737:chemistry*; D012997:Solvents; D013237:Stereoisomerism
chemical_list: D000475:Alkenes; D005656:Fungal Proteins; D008024:Ligands; D010080:Oxazoles; D011108:Polymers; D012997:Solvents; D042845:Laccase; D009195:Peroxidase; D008049:Lipase; D009113:Muramidase; D002918:Chymotrypsin; C117608:alpha-chymotrypsin
keywords: Sharpless dihydroxylation; artificial metalloenzymes; asymmetric catalysis; organic solvents; polymer enzyme conjugates