title: Chemotaxis-based endosulfan biotransformation: enrichment and isolation of endosulfan-degrading bacteria.
abstract: The study was conducted to isolate endosulfan biotransforming or biodegrading microbes based on chemotaxis. Pseudomonas aeruginosa strain KKc3, Ochrobactrum sp. strain KKc4, Achromobacter xylosoxidans strain KKc6 and Bacillus megaterium KKc7 were isolated based on their migration towards endosulfan in a soil column. Out of the four bacteria, B. megaterium converted endosulfan into toxic metabolite endosulfan sulphate, while the other three bacteria followed the non-toxic endosulfan diol pathway. The mixed culture system consisting of P. aeruginosa, Ochrobactrum sp and A. xylosoxidans could remove 94% of total endosulfan by using endosulfan as the sole source of sulphur.
authors.affiliation: a Environmental Health Division , National Environmental Engineering Research Institute , Nagpur 440022 , India.
authors.forename: Muhil Vannan
authors.initials: MV
authors.lastname: Seralathan
authors.forename: Saravanadevi
authors.initials: S
authors.lastname: Sivanesan
authors.forename: Srinivasan
authors.initials: S
authors.lastname: Nargunanathan
authors.forename: Amit
authors.initials: A
authors.lastname: Bafana
authors.forename: Krishnamurthi
authors.initials: K
authors.lastname: Kannan
authors.forename: Tapan
authors.initials: T
authors.lastname: Chakrabarti
mesh_terms: D001419:Bacteria / Q000302:isolation & purification* / Q000378:metabolism*; D061249:Batch Cell Culture Techniques / Q000295:instrumentation / Q000379:methods; D001673:Biodegradation, Environmental; D019149:Bioreactors / Q000382:microbiology; D001711:Biotransformation; D002633:Chemotaxis / Q000502:physiology*; D004726:Endosulfan / Q000302:isolation & purification / Q000378:metabolism*; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D012989:Soil Pollutants; D004726:Endosulfan
keywords: DGGE; biotransformation; chemotaxis; endosulfan; kinetics