title: Ultra-performance liquid chromatography quadrupole time-of-flight MS for identification of electron beam from accelerator degradation products of aflatoxin B1.
abstract: Electron beam irradiation was proven to be a successful method in aflatoxin degradation in earlier researches. However, the exact nature of the result radiation products generated by the aflatoxins remains unknown. Based on ultra-performance liquid chromatography quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF MS) analysis, the solution of aflatoxin B1 (AFB1) in acetonitrile irradiated by electron beam degraded to two kinds of major products. The doses employed were in the range of 0 (control) to 8.60 kGy. The absorbed doses were monitored with FWT-60-00 radio-chromic dosimeters. By using UPLC-Q-TOF MS, accurate masses and proposed molecular formula for the degradation products, 261.1233 m/z (C14H13O5) and 299.1104 m/z (C17H15O5), were obtained from low mass error and high matching properties. Structural formula for the radio-degradation products and the degradation pathways leading to the compounds were proposed, based on the molecular formula and MS-MS spectra. The results showed that electron beam (EB) irradiation is an effective method for degrading AFB1.
authors.affiliation: State Key Laboratory of Food Science and Technology, Synergetic Innovation Center of Food Safety and Nutrition, School of Food Science and Technology, Jiangnan University, 1800 Lihu Road, Wuxi, 214122, Jiangsu Province, People's Republic of China.
authors.forename: Ruiqi
authors.initials: R
authors.lastname: Wang
authors.forename: Ruijie
authors.initials: R
authors.lastname: Liu
authors.forename: Ming
authors.initials: M
authors.lastname: Chang
authors.forename: Qingzhe
authors.initials: Q
authors.lastname: Jin
authors.forename: Jianhua
authors.initials: J
authors.lastname: Huang
authors.forename: Yuanfa
authors.initials: Y
authors.lastname: Liu
authors.forename: Xingguo
authors.initials: X
authors.lastname: Wang
mesh_terms: D016604:Aflatoxin B1 / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004583:Electrons; D007477:Ions; D007700:Kinetics; D013058:Mass Spectrometry / Q000379:methods*; D008970:Molecular Weight; D013997:Time Factors
chemical_list: D007477:Ions; D016604:Aflatoxin B1