title: Long-term thiol monitoring in living cells using bioorthogonal chemistry.
abstract: Intracellular thiols play vital roles in living systems, and their in situ monitoring is of great importance. Here, we report on a bioorthogonal chemistry based fluorescent probe, which is capable of monitoring intracellular thiols in living cells for up to 36 hours with an obvious blue-to-green fluorescence change.
authors.affiliation: Key Laboratory of Biomedical Polymers of Ministry of Education & Department of Chemistry, Wuhan University, Wuhan 430072, P. R. China. xz-zhang@whu.edu.cn.
authors.forename: Lei
authors.initials: L
authors.lastname: Rong
authors.forename: Chi
authors.initials: C
authors.lastname: Zhang
authors.forename: Qi
authors.initials: Q
authors.lastname: Lei
authors.forename: Hua-Ling
authors.initials: HL
authors.lastname: Sun
authors.forename: Si-Yong
authors.initials: SY
authors.lastname: Qin
authors.forename: Jun
authors.initials: J
authors.lastname: Feng
authors.forename: Xian-Zheng
authors.initials: XZ
authors.lastname: Zhang
mesh_terms: D002470:Cell Survival; D005456:Fluorescent Dyes / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D053644:Naphthalimides / Q000737:chemistry*; D061848:Optical Imaging; D013438:Sulfhydryl Compounds / Q000032:analysis*
chemical_list: D005456:Fluorescent Dyes; D053644:Naphthalimides; D013438:Sulfhydryl Compounds