title: Transcriptome-wide profiling and expression analysis of diploid and autotetraploid Paulownia tomentosa × Paulownia fortunei under drought stress.
abstract: Paulownia is a fast-growing deciduous hardwood species native to China, which has high ecological and economic value. In an earlier study, we reported ploidy-dependent differences in Paulownia drought tolerance by the microscopic observations of the leaves. Autotetraploid Paulownia has a higher resistance to drought stress than their diploid relatives. In order to obtain genetic information on molecular mechanisms responses of Paulownia plants to drought, Illumina/Solexa Genome sequencing platform was used to de novo assemble the transcriptomes of leaves from diploid and autotetraploid Paulownia tomentosa × Paulownia fortunei seedlings (PTF2 and PTF4 respectively) grown under control conditions and under drought stress and obtained 98,671 nonredundant unigenes. A comparative transcriptome analysis revealed that hundreds of unigenes were predicted to be involved mainly in ROS-scavenging system, amino acid and carbohydrate metabolism, plant hormone biosynthesis and signal transduction, while these unigenes exhibited differential transcript alteration of the two accessions. This study provides a comprehensive map of how P. tomentosa × P. fortunei responds to drought stress at physiological and molecular levels, which may help in understanding the mechanisms involve in water-deficit response and will be useful for further study of drought tolerance in woody plants.
authors.affiliation: Institute of Paulownia, Henan Agricultural University, Jinshui Area, Zhengzhou, Henan, P.R. China; College of Forestry, Henan Agricultural University, Jinshui Area, Zhengzhou, Henan, P.R. China.
authors.forename: Enkai
authors.initials: E
authors.lastname: Xu
authors.affiliation: Institute of Paulownia, Henan Agricultural University, Jinshui Area, Zhengzhou, Henan, P.R. China; College of Forestry, Henan Agricultural University, Jinshui Area, Zhengzhou, Henan, P.R. China.
authors.forename: Guoqiang
authors.initials: G
authors.lastname: Fan
authors.affiliation: Institute of Paulownia, Henan Agricultural University, Jinshui Area, Zhengzhou, Henan, P.R. China; College of Forestry, Henan Agricultural University, Jinshui Area, Zhengzhou, Henan, P.R. China.
authors.forename: Suyan
authors.initials: S
authors.lastname: Niu
authors.affiliation: Institute of Paulownia, Henan Agricultural University, Jinshui Area, Zhengzhou, Henan, P.R. China; College of Forestry, Henan Agricultural University, Jinshui Area, Zhengzhou, Henan, P.R. China.
authors.forename: Zhenli
authors.initials: Z
authors.lastname: Zhao
authors.affiliation: Institute of Paulownia, Henan Agricultural University, Jinshui Area, Zhengzhou, Henan, P.R. China; College of Forestry, Henan Agricultural University, Jinshui Area, Zhengzhou, Henan, P.R. China.
authors.forename: Minjie
authors.initials: M
authors.lastname: Deng
authors.affiliation: Institute of Paulownia, Henan Agricultural University, Jinshui Area, Zhengzhou, Henan, P.R. China; College of Forestry, Henan Agricultural University, Jinshui Area, Zhengzhou, Henan, P.R. China.
authors.forename: Yanpeng
authors.initials: Y
authors.lastname: Dong
mesh_terms: D000222:Adaptation, Physiological / Q000235:genetics; D003433:Crosses, Genetic; D004171:Diploidy; D055864:Droughts; D020869:Gene Expression Profiling / Q000379:methods*; D018506:Gene Expression Regulation, Plant; D063990:Gene Ontology; D053263:Gene Regulatory Networks / Q000235:genetics; D019684:Magnoliopsida / Q000235:genetics*; D018515:Plant Leaves / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D036226:Seedlings / Q000235:genetics; D015398:Signal Transduction / Q000235:genetics; D013312:Stress, Physiological / Q000235:genetics; D057891:Tetraploidy