title: ROG1 encodes a monoacylglycerol lipase in Saccharomyces cerevisiae.
abstract: Lipid metabolism is extensively studied in Saccharomyces cerevisiae. Here, we report that revertant of glycogen synthase kinase mutation-1 (Rog1p) possesses monoacylglycerol (MAG) lipase activity in S. cerevisiae. The lipase activity of Rog1p was confirmed in two ways: through analysis of a strain with a double deletion of ROG1 and monoglyceride lipase YJU3 (yju3Δrog1Δ) and by site-directed mutagenesis of the ROG1 lipase motif (GXSXG). Rog1p is localized in both the cytosol and the nucleus. Overexpression of ROG1 in a ROG1-deficient strain resulted in an accumulation of reactive oxygen species. These results suggest that Rog1p is a MAG lipase that regulates lipid homeostasis.
authors.affiliation: Department of Biochemistry, School of Life Sciences, Bharathidasan University, Tiruchirappalli 620 024, Tamil Nadu, India.
authors.forename: Lakshmanaperumal
authors.initials: L
authors.lastname: Vishnu Varthini
authors.affiliation: Department of Biochemistry, School of Life Sciences, Bharathidasan University, Tiruchirappalli 620 024, Tamil Nadu, India.
authors.forename: Kandasamy
authors.initials: K
authors.lastname: Selvaraju
authors.affiliation: CSIR-Central Food Technological Research Institute, Lipidomics Center, Allalasandra, GKVK Post, Bellary Road, Bangalore 560 065, Karnataka, India.
authors.forename: Malathi
authors.initials: M
authors.lastname: Srinivasan
authors.affiliation: Department of Biochemistry, School of Life Sciences, Bharathidasan University, Tiruchirappalli 620 024, Tamil Nadu, India. Electronic address: vasanthibch@gmail.com.
authors.forename: Vasanthi
authors.initials: V
authors.lastname: Nachiappan
mesh_terms: D020816:Amino Acid Motifs; D017353:Gene Deletion; D008994:Monoacylglycerol Lipases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D016297:Mutagenesis, Site-Directed; D017382:Reactive Oxygen Species / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry / Q000378:metabolism*
chemical_list: D017382:Reactive Oxygen Species; D029701:Saccharomyces cerevisiae Proteins; D008994:Monoacylglycerol Lipases
keywords: Lipid droplet; Lipid metabolism; Monoacylglycerol; Monoacylglycerol lipase; Reactive oxygen species; Saccharomyces cerevisiae