title: ATPase may play a critical role in disturbance of energy metabolism in congestive heart failure rats.
abstract: OBJECTIVE
  This study aims to explore the expression and significance of ATPase in myocardium of congestive heart failure induced by pressure overload in rats.
  
  
  METHODS
  Male SD rats were divided into 3 groups randomly: Control group (C group), coarctation of abdominal aorta 15 weeks group (A group) and sham operation group (SH group). The hemodynamics parameters were measured. The size of adenine acid pool (ATP, ADP) in myocardium was measured by high performance liquid chromatography (HPLC). The mRNA copies of ATPase-α subunit, ATPase-6 subunit in ventricular myocardium were determined by RT-PCR. The protein content of F1-ATPase in mitochondria was detected by Western blot analysis.
  
  
  RESULTS
  The ±dp/dtmax and contents of ATP and ADP decreased significantly in myocardium were decreased in A group. Compared with C and SH group, the mRNA copies of ATPase-α subunit, ATPase-6 subunit and the protein content of F1-ATPase were decreased in A group.
  
  
  CONCLUSIONS
  The expression of F1-ATPase decreased parallel with abnormal of the adenine nucleotide in myocardium of congestive heart failure. It may play a critical role in disturbance of energy metabolism in disfunction of heart.
authors.affiliation: Department of Intensive Care Unit, The Fifth Hospital of PLA Yinchuan 750004, Ningxia, P. R. China.
authors.forename: Rong
authors.initials: R
authors.lastname: Song
authors.affiliation: Department of Cardiology, Xinqiao Hospital, The Third Military Medical University Chongqing 400038, P. R. China.
authors.forename: Shanjun
authors.initials: S
authors.lastname: Zhu
authors.affiliation: Department of Cardiology, The General Hospital of Jinan Military Region Jinan 250031, Shandong, P. R. China.
authors.forename: Hongming
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Department of Cardiology, The General Hospital of Jinan Military Region Jinan 250031, Shandong, P. R. China.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Cardiology, Renmin Hospital of Wuhan University Wuhan 430060, Hubei, P. R. China.
authors.forename: Songlin
authors.initials: S
authors.lastname: Wu
keywords: ATPase; Congestive heart failure; mitochondria