title: Live cell imaging of the cytoskeleton and cell wall enzymes in plant cells.
abstract: The use of live imaging techniques to visualize the dynamic changes and interactions within plant cells has given us detailed information on the function and organization of the cytoskeleton and cell wall associated proteins. This information has grown with the constant improvement in imaging hardware and molecular tools. In this chapter, we describe the procedure for the preparation and live visualization of fluorescent protein fusions associated with the cytoskeleton and the cell wall in Arabidopsis.
authors.affiliation: Division of Biology and Biological Engineering 156-29, California Institute of Technology, Pasadena, CA, 91125, USA, arun.sampathkumar@gmail.com.
authors.forename: Arun
authors.initials: A
authors.lastname: Sampathkumar
authors.forename: Raymond
authors.initials: R
authors.lastname: Wightman
mesh_terms: D017360:Arabidopsis / Q000166:cytology* / Q000201:enzymology*; D029681:Arabidopsis Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D002473:Cell Wall / Q000201:enzymology; D003599:Cytoskeleton / Q000201:enzymology; D049452:Green Fluorescent Proteins / Q000378:metabolism; D047569:Laser Scanning Cytometry / Q000379:methods*; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism
chemical_list: D029681:Arabidopsis Proteins; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins