title: Extraction and Quantification of Cyclic Di-GMP fromP. aeruginosa.
abstract: Cyclic di-GMP (c-di-GMP) has emerged as an important intracellular signaling molecule, controlling the transitions between planktonic (free-living) and sessile lifestyles, biofilm formation, and virulence in a wide variety of microorganisms. The following protocol describes the extraction and quantification of c-di-GMP fromPseudomonas aeruginosasamples. We have made every effort to keep the protocol as general as possible to enable the procedure to be applicable for the analysis of c-di-GMP levels in various bacterial species. However, some modifications may be required for the analysis of c-di-GMP levels in other bacterial species.
authors.affiliation: Department of Biological Sciences, Binghamton University, Binghamton, NY 13902.
authors.forename: Ankita Basu
authors.initials: AB
authors.lastname: Roy
authors.affiliation: Department of Biological Sciences, Binghamton University, Binghamton, NY 13902.
authors.forename: Olga E
authors.initials: OE
authors.lastname: Petrova
authors.affiliation: Department of Biological Sciences, Binghamton University, Binghamton, NY 13902.
authors.forename: Karin
authors.initials: K
authors.lastname: Sauer