title: Evaluation of hypoglycemic efficacy of tangningtongluo formula, a traditional Chinese Miao medicine, in two rodent animal models.
abstract: Traditional Chinese medicines largely lack adequate and scientifically rigorous evidence regarding efficacy and functional mechanisms. The present study was aimed to confirm the hypoglycemic effect of Tangningtongluo (TNTL) formula, a traditional Chinese Miao medicine, in two animal models: high-fat diet and streptozotocin- (STZ-) induced diabetic rats and C57BL/KsJ-db/db diabetic mice. After 4 weeks, TNTL intervention in STZ-induced diabetic rats yielded in significant improvement on the glucose tolerance test. Moreover, the islet histopathology showed that oral TNTL reduced the severity of islet necrosis in pancreases tissue. Compared with diabetic controls, a 12-week TNTL treatment regimen (dosages = 0.9, 1.8, and 3.6 g/kg) in db/db mice significantly decreased fasting glucose and HbA1c. Additionally, oral glucose tolerance in TNTL-treated mice improved significantly, compared with diabetic mice receiving metformin. Finally, tissue histopathology and biochemical index evaluations revealed significant improvement in TNTL-treated mice. Taken together, our results show that TNTL exerted a strong hypoglycemic effect in two diabetic rodent animal models, preserving β-cells in the pancreas islet and reducing the risk of diabetic retinopathy and nephropathy.
authors.affiliation: Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Ministry of Education, No. 151, Malianwa North Road, Haidian District, Beijing 100193, China.
authors.forename: Long
authors.identifier: 0000-0002-8933-4730
authors.initials: L
authors.lastname: Cheng
authors.affiliation: Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Ministry of Education, No. 151, Malianwa North Road, Haidian District, Beijing 100193, China.
authors.forename: Xiang-bao
authors.initials: XB
authors.lastname: Meng
authors.affiliation: Heilongjiang University of Chinese Medicine, Harbin, Heilongjiang 150040, China.
authors.forename: Shan
authors.initials: S
authors.lastname: Lu
authors.affiliation: Pharmaceutical College, Harbin University of Commerce, Harbin, Heilongjiang 150076, China.
authors.forename: Ting-ting
authors.initials: TT
authors.lastname: Wang
authors.affiliation: Xiyuan Hospital, China Academy of Traditional Chinese Medicine, Beijing 100091, China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Ministry of Education, No. 151, Malianwa North Road, Haidian District, Beijing 100193, China.
authors.forename: Gui-bo
authors.initials: GB
authors.lastname: Sun
authors.affiliation: Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Ministry of Education, No. 151, Malianwa North Road, Haidian District, Beijing 100193, China.
authors.forename: Xiao-bo
authors.initials: XB
authors.lastname: Sun
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000097:blood; D001786:Blood Glucose / Q000187:drug effects / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental / Q000097:blood / Q000139:chemically induced / Q000188:drug therapy* / Q000473:pathology; D003928:Diabetic Nephropathies / Q000209:etiology / Q000517:prevention & control; D003930:Diabetic Retinopathy / Q000209:etiology / Q000517:prevention & control; D059305:Diet, High-Fat; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D006442:Glycated Hemoglobin / Q000378:metabolism; D007004:Hypoglycemic Agents / Q000494:pharmacology*; D007515:Islets of Langerhans / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D008297:Male; D008687:Metformin / Q000494:pharmacology; D008810:Mice, Inbred C57BL; D009336:Necrosis; D017207:Rats, Sprague-Dawley; D013311:Streptozocin; D013997:Time Factors
chemical_list: D015415:Biomarkers; D001786:Blood Glucose; D004365:Drugs, Chinese Herbal; D006442:Glycated Hemoglobin; D007004:Hypoglycemic Agents; D008687:Metformin; D013311:Streptozocin; C517652:hemoglobin A1c protein, human; C000599174:tangningtongluo