title: Synthesis of fluorescent dye-doped silica nanoparticles for target-cell-specific delivery and intracellular microRNA imaging.
abstract: MicroRNA (miRNA) is found to be up-regulated in many kinds of cancer and therefore is classified as an oncomiR. Herein, we design a multifunctional fluorescent nanoprobe (FSiNP-AS/MB) with the AS1411 aptamer and a molecular beacon (MB) co-immobilized on the surface of the fluorescent dye-doped silica nanoparticles (FSiNPs) for target-cell-specific delivery and intracellular miRNA imaging. The FSiNPs were prepared by a facile reverse microemulsion method from tetraethoxysilane and silane derivatized coumarin that was previously synthesized by click chemistry. The as-prepared FSiNPs possess uniform size distribution, good optical stability and biocompatibility. In addition, there is a remarkable affinity interaction between the AS1411 aptamer and the nucleolin protein on the cancer cell surface. Thus, a target-cell-specific delivery system by the FSiNP-AS/MB is proposed for effectively transferring a MB into the cancer cells to recognize the target miRNA. Using miRNA-21 in MCF-7 cells (a human breast cancer cell line) as a model, the proposed multifunctional nanosystems not only allow target-cell-specific delivery with the binding affinity of AS1411, but also can track simultaneously the transfected cells and detect intracellular miRNA in situ. The proposed multifunctional nanosystems are a promising platform for a highly sensitive luminescent nonviral vector in biomedical and clinical research.
authors.affiliation: School of Chemistry and Chemical Engineering, Southeast University, Jiangning District, Nanjing, 211189, P. R. China. liusq@seu.edu.cn.
authors.forename: Henan
authors.initials: H
authors.lastname: Li
authors.forename: Yawen
authors.initials: Y
authors.lastname: Mu
authors.forename: Shanshan
authors.initials: S
authors.lastname: Qian
authors.forename: Jusheng
authors.initials: J
authors.lastname: Lu
authors.forename: Yakun
authors.initials: Y
authors.lastname: Wan
authors.forename: Guodong
authors.initials: G
authors.lastname: Fu
authors.forename: Songqin
authors.initials: S
authors.lastname: Liu
mesh_terms: D052157:Aptamers, Nucleotide / Q000378:metabolism; D045744:Cell Line, Tumor; D003374:Coumarins / Q000737:chemistry; D003952:Diagnostic Imaging / Q000379:methods; D005456:Fluorescent Dyes / Q000737:chemistry; D006801:Humans; D061986:MCF-7 Cells; D035683:MicroRNAs / Q000032:analysis* / Q000235:genetics*; D053758:Nanoparticles; D009369:Neoplasms / Q000175:diagnosis* / Q000378:metabolism; D009838:Oligodeoxyribonucleotides / Q000378:metabolism; D010750:Phosphoproteins / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000378:metabolism; D012821:Silanes / Q000737:chemistry; D000097796:Nucleolin
chemical_list: D052157:Aptamers, Nucleotide; D003374:Coumarins; D005456:Fluorescent Dyes; D035683:MicroRNAs; D009838:Oligodeoxyribonucleotides; D010750:Phosphoproteins; D016601:RNA-Binding Proteins; D012821:Silanes; D000097796:Nucleolin; C513936:AGRO 100; C524940:MIRN21 microRNA, human; C040733:tetraethoxysilane; C030123:coumarin