title: Transplanted Human Amniotic Epithelial Cells Secrete Paracrine Proangiogenic Cytokines in Rat Model of Myocardial Infarction.
abstract: Human amniotic epithelial cells (h-AECs) have been shown to differentiate into cardiomyocyte-like cells in vivo that can regenerate myocardial tissue and improve cardiac function in a rat model of myocardial infarction (MI). In this study, we investigated the paracrine factors released from h-AECs under hypoxic conditions to elucidate the possible mechanisms underlying this previously reported phenomenon of h-AEC-mediated cardiac repair. We used hypoxic cell culture conditions to simulate myocardial infarction in vitro. In comparison to normal conditions, we found that h-AECs secreted higher levels of several cytokines, including angiogenin (ANG), epidermal growth factor (EGF), interleukin (IL)-6, and monocyte chemoattractant protein (MCP)-1. To determine whether transplanted h-AECs express these proangiogenic cytokines in vivo, we ligated the coronary artery of rats to cause MI and injected either h-AECs or saline into the infarcted area. We found that the infarct and border zones of rat myocardium treated with h-AECs had higher expression levels of the human-origin cytokines ANG, EGF, IL-6, and MCP-1 compared to the tissues of saline-treated rats. In conclusion, h-AECs secreted proangiogenic cytokines in a rat model of MI, which may suggest that the paracrine effect by h-AECs could regenerate myocardial tissue and improve cardiac function.
authors.affiliation: Graduate School of Biomedical Science and Engineering, Hanyang University, Seoul, South Korea.
authors.forename: Yi-Sun
authors.initials: YS
authors.lastname: Song
authors.forename: Hyun-Woo
authors.initials: HW
authors.lastname: Joo
authors.forename: In-Hwa
authors.initials: IH
authors.lastname: Park
authors.forename: Guang-Yin
authors.initials: GY
authors.lastname: Shen
authors.forename: Yonggu
authors.initials: Y
authors.lastname: Lee
authors.forename: Jeong Hun
authors.initials: JH
authors.lastname: Shin
authors.forename: Hyuck
authors.initials: H
authors.lastname: Kim
authors.forename: Il-Seob
authors.initials: IS
authors.lastname: Shin
authors.forename: Kyung-Soo
authors.initials: KS
authors.lastname: Kim
mesh_terms: D000650:Amnion / Q000166:cytology*; D000818:Animals; D002454:Cell Differentiation / Q000502:physiology; D017690:Cell Transplantation / Q000379:methods; D003331:Coronary Vessels / Q000378:metabolism; D016207:Cytokines / Q000378:metabolism; D004195:Disease Models, Animal; D004847:Epithelial Cells / Q000166:cytology*; D006801:Humans; D009203:Myocardial Infarction / Q000628:therapy*; D009206:Myocardium / Q000378:metabolism; D032383:Myocytes, Cardiac / Q000166:cytology*; D018919:Neovascularization, Physiologic / Q000502:physiology*; D051381:Rats; D016277:Ventricular Function, Left / Q000502:physiology
chemical_list: D016207:Cytokines