title: Thermodynamic, kinetic and conformational analysis of proteins diffusion-sorption on a solid surface.
abstract: In this paper we examine particularly some of the more fundamental properties of protein conformational changes at a solid surface coupled with diffusion from the bulk of an aqueous solution and with the adsorption-desorption processes. We focus our attention on adsorbed protein monolayers upon a solid surface using a thermodynamic and kinetic analytical development. Account is also taken of the effects on the overall rate of the conformational change on a solid surface of deviation from ideality, of protein flexibility, of surface free energy and of interaction with reactive solid sites. Our theory applied to steady states is illustrated by examples such as folding-misfolding-unfolding of RNase and SNase on a solid surface after diffusion and adsorption from an aqueous solution. For this purpose, we put forward the determining steps which shall lead to the steady state. The existence of three situations is highlighted according to the values of the typical constants relevant for the protein considered: reaction rate determining step, diffusion and sorption determining steps, mixed adsorption diffusion and reaction rate. Finally, we have tried to link the developments of our theories to a large literature based on experimental results encountered during proteins diffusion-sorption-reaction processes, fundamental topics that has been since long investigated by Miller's team in MPKG.
authors.affiliation: MADIREL UMR 7246 Aix-Marseille University, Bd Escadrille Normandie Niemen, 13397, Marseille Cedex 20, France.
authors.forename: Albert
authors.initials: A
authors.lastname: Sanfeld
authors.affiliation: Centre de Biochimie Structurale, INSERM U554, CNRS UMR 5048, 29, rue de Navacelles, 34090 Montpellier, France.
authors.forename: Catherine
authors.initials: C
authors.lastname: Royer
authors.affiliation: MADIREL UMR 7246 Aix-Marseille University, Bd Escadrille Normandie Niemen, 13397, Marseille Cedex 20, France.
authors.forename: Annie
authors.initials: A
authors.lastname: Steinchen
mesh_terms: D000327:Adsorption; D004058:Diffusion; D007700:Kinetics; D011487:Protein Conformation; D011506:Proteins / Q000737:chemistry*; D013816:Thermodynamics
chemical_list: D011506:Proteins
keywords: Conformational change; Diffusion; Proteins; Solid surface; Sorption