title: Transcriptome-wide variability in single embryonic development cells.
abstract: Molecular heterogeneity of individual molecules within single cells has been recently shown to be crucial for cell fate diversifications. However, on a global scale, the effect of molecular variability for embryonic developmental stages is largely underexplored. Here, to understand the origins of transcriptome-wide variability of oocytes to blastocysts in human and mouse, we examined RNA-Seq datasets. Evaluating Pearson correlation, Shannon entropy and noise patterns (η(2) vs. μ), our investigations reveal a phase transition from low to saturating levels of diversity and variability of transcriptome-wide expressions through the development stages. To probe the observed behaviour further, we utilised a stochastic transcriptional model to simulate the global gene expressions pattern for each development stage. From the model, we concur that transcriptome-wide regulation initially begins from 2-cell stage, and becomes strikingly variable from 8-cell stage due to amplification and quantal transcriptional activity.
authors.affiliation: 1] Institute for Advanced Biosciences, Keio University, 14-1 Baba-cho, 997-0035, Tsuruoka, Japan [2] Systems Biology Program, Graduate School of Media and Governance, Keio University, 5322 Endo, 252-0882, Fujisawa, Japan.
authors.forename: Vincent
authors.initials: V
authors.lastname: Piras
authors.affiliation: 1] Institute for Advanced Biosciences, Keio University, 14-1 Baba-cho, 997-0035, Tsuruoka, Japan [2] Systems Biology Program, Graduate School of Media and Governance, Keio University, 5322 Endo, 252-0882, Fujisawa, Japan.
authors.forename: Masaru
authors.initials: M
authors.lastname: Tomita
authors.affiliation: 1] Institute for Advanced Biosciences, Keio University, 14-1 Baba-cho, 997-0035, Tsuruoka, Japan [2] Systems Biology Program, Graduate School of Media and Governance, Keio University, 5322 Endo, 252-0882, Fujisawa, Japan.
authors.forename: Kumar
authors.initials: K
authors.lastname: Selvarajoo
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000166:cytology / Q000378:metabolism*; D002454:Cell Differentiation; D004622:Embryo, Mammalian; D047108:Embryonic Development / Q000235:genetics; D053595:Embryonic Stem Cells / Q000166:cytology / Q000378:metabolism*; D019277:Entropy; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D014644:Genetic Variation; D059014:High-Throughput Nucleotide Sequencing; D006801:Humans; D051379:Mice; D015233:Models, Statistical; D009865:Oocytes / Q000166:cytology / Q000378:metabolism*; D017423:Sequence Analysis, RNA; D059010:Single-Cell Analysis; D013269:Stochastic Processes; D059467:Transcriptome