title: Exploring processing adjuvants' influence on traditional Chinese medicine compatibility of Aconiti Radix Cocta and Pinelliae rhizome using rapid resolution liquid chromatography-quadrupole time-of-flight mass spectrometry.
abstract: BACKGROUND
  It is known that when crude Pinelliae rhizome and Pinelliae rhizoma preparatum are combined with Aconiti Radix Cocta respectively, the toxicity of the combination varies. However, the component's transformation between different compatibility have remained unclear.
  
  
  OBJECTIVE
  In this paper, a novel approach using rapid resolution liquid chromatography-quadrupole time-of-flight mass spectrometry (RRLC-Q-TOF-MS) coupled with multivariate statistical analysis was established for exploring the influence of processing adjuvants (PAs) on the compatibility of Aconiti Radix Cocta and Pinelliae rhizome.
  
  
  METHODS
  In order to obtain information about the representative markers between different groups, an exhaustive study of different protocols based on adding or removing different PAs step by step was carried out and the influence of PAs on compatibility was investigated.
  
  
  RESULTS
  It was found that lime can facilitate diester diterpenoid alkaloids with high toxicity in Aconiti Radix Cocta to be converted into low-toxic or non-toxic derivatives. Glycyrrhizae Radix et Rhizoma had no remarkable effect on the process.
  
  
  CONCLUSIONS
  The established method in this study will be of great significance to process research mechanism and study on traditional Chinese Medicine compatibility and clinical application.
authors.affiliation: Tianjin State Key Laboratory of Modern Chinese Medicine, School of Traditional Chinese Materia Medica, Tianjin University of TCM, Tianjin 300193, China.
authors.forename: Yuming
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Tianjin State Key Laboratory of Modern Chinese Medicine, School of Traditional Chinese Materia Medica, Tianjin University of TCM, Tianjin 300193, China.
authors.forename: Yubo
authors.initials: Y
authors.lastname: Li
authors.affiliation: Tianjin State Key Laboratory of Modern Chinese Medicine, School of Traditional Chinese Materia Medica, Tianjin University of TCM, Tianjin 300193, China.
authors.forename: Xiuxiu
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Tianjin State Key Laboratory of Modern Chinese Medicine, School of Traditional Chinese Materia Medica, Tianjin University of TCM, Tianjin 300193, China.
authors.forename: Yanyan
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Tianjin State Key Laboratory of Modern Chinese Medicine, School of Traditional Chinese Materia Medica, Tianjin University of TCM, Tianjin 300193, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Wang
authors.affiliation: Tianjin State Key Laboratory of Modern Chinese Medicine, School of Traditional Chinese Materia Medica, Tianjin University of TCM, Tianjin 300193, China.
authors.forename: Yanjun
authors.initials: Y
authors.lastname: Zhang
keywords: Aconiti Radix Cocta; Pinelliae rhizome; RRLC-Q-TOF-MS; processing adjuvants