title: Defined patterns of neuronal networks on 3D thiol-functionalized microstructures.
abstract: It is very challenging to study the behavior of neuronal cells in a network due to the multiple connections between the cells. Our idea is then to simplify such a network with a configuration where cells can have just a fixed number of connections in order to create a well-defined and ordered network. Here, we report about guiding primary cortical neurons with three-dimensional gold microspines selectively functionalized with an amino-terminated molecule.
authors.affiliation: Institute of Bioelectronics ICS-8/PGI-8, Forschungszentrum Jülich , 52425 Jülich, Germany.
authors.forename: Francesca
authors.initials: F
authors.lastname: Santoro
authors.forename: Gregory
authors.initials: G
authors.lastname: Panaitov
authors.forename: Andreas
authors.initials: A
authors.lastname: Offenhäusser
mesh_terms: D000818:Animals; D061249:Batch Cell Culture Techniques / Q000379:methods; D002448:Cell Adhesion / Q000502:physiology; D002478:Cells, Cultured; D006046:Gold / Q000737:chemistry; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D051379:Mice; D054802:Molecular Imprinting / Q000379:methods*; D009415:Nerve Net / Q000166:cytology* / Q000502:physiology; D009474:Neurons / Q000166:cytology* / Q000502:physiology; D010316:Particle Size; D013438:Sulfhydryl Compounds / Q000737:chemistry*; D046888:Tissue Array Analysis / Q000379:methods*
chemical_list: D013438:Sulfhydryl Compounds; D006046:Gold
keywords: 3D microstructures; cell guidance; cortical neurons; patterning; self-assembled monolayer