title: Periadventitial adipose-derived stem cell treatment halts elastase-induced abdominal aortic aneurysm progression.
abstract: OBJECTIVE
  Demonstrate that periadventitial delivery of adipose-derived mesenchymal stem cells (ADMSCs) slows aneurysm progression in an established murine elastase-perfusion model of abdominal aortic aneurysm (AAA).
  
  
  METHODS
  AAAs were induced in C57BL/6 mice using porcine elastase. During elastase perfusion, a delivery device consisting of a subcutaneous port, tubing and porous scaffold was implanted. Five days after elastase perfusion, 100,000 ADMSCs were delivered through the port to the aorta. After sacrifice at day 14, analyzed metrics included aortic diameter and structure of aortic elastin.
  
  
  RESULTS
  ADMSC treated aneurysms had a smaller diameter and less fragmented elastin versus saline controls.
  
  
  CONCLUSIONS
  Periadventitial stem cell delivery prevented the expansion of an established aneurysm between days 5 and 14 after elastase perfusion.
authors.affiliation: Department of Bioengineering, University of Pittsburgh, Pittsburgh, PA, USA.
authors.forename: Kory J
authors.initials: KJ
authors.lastname: Blose
authors.forename: Terri L
authors.initials: TL
authors.lastname: Ennis
authors.forename: Batool
authors.initials: B
authors.lastname: Arif
authors.forename: Justin S
authors.initials: JS
authors.lastname: Weinbaum
authors.forename: John A
authors.initials: JA
authors.lastname: Curci
authors.forename: David A
authors.initials: DA
authors.lastname: Vorp
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D063194:Adventitia / Q000166:cytology*; D000818:Animals; D017544:Aortic Aneurysm, Abdominal / Q000209:etiology / Q000473:pathology / Q000517:prevention & control*; D002454:Cell Differentiation; D002478:Cells, Cultured; D004195:Disease Models, Animal; D018450:Disease Progression; D005455:Fluorescent Antibody Technique; D007091:Image Processing, Computer-Assisted; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D051379:Mice; D008810:Mice, Inbred C57BL; D010196:Pancreatic Elastase / Q000009:adverse effects*; D013552:Swine
chemical_list: D010196:Pancreatic Elastase
keywords: abdominal aortic aneurysm; adipose-derived stem cells; elastin; regeneration