title: An integrated biotechnology platform for developing sustainable chemical processes.
abstract: Genomatica has established an integrated computational/experimental metabolic engineering platform to design, create, and optimize novel high performance organisms and bioprocesses. Here we present our platform and its use to develop E. coli strains for production of the industrial chemical 1,4-butanediol (BDO) from sugars. A series of examples are given to demonstrate how a rational approach to strain engineering, including carefully designed diagnostic experiments, provided critical insights about pathway bottlenecks, byproducts, expression balancing, and commercial robustness, leading to a superior BDO production strain and process.
authors.affiliation: , Genomatica, Inc., 4757 Nexus Center Drive, San Diego, CA, 92121, USA.
authors.forename: Nelson R
authors.initials: NR
authors.lastname: Barton
authors.forename: Anthony P
authors.initials: AP
authors.lastname: Burgard
authors.forename: Mark J
authors.initials: MJ
authors.lastname: Burk
authors.forename: Jason S
authors.initials: JS
authors.lastname: Crater
authors.forename: Robin E
authors.initials: RE
authors.lastname: Osterhout
authors.forename: Priti
authors.initials: P
authors.lastname: Pharkya
authors.forename: Brian A
authors.initials: BA
authors.lastname: Steer
authors.forename: Jun
authors.initials: J
authors.lastname: Sun
authors.forename: John D
authors.initials: JD
authors.lastname: Trawick
authors.forename: Stephen J
authors.initials: SJ
authors.lastname: Van Dien
authors.forename: Tae Hoon
authors.initials: TH
authors.lastname: Yang
authors.forename: Harry
authors.initials: H
authors.lastname: Yim
mesh_terms: D001709:Biotechnology / Q000379:methods*; D002072:Butylene Glycols / Q000378:metabolism; D002247:Carbon Isotopes; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics / Q000378:metabolism; D005285:Fermentation; D055772:Green Chemistry Technology; D060847:Metabolic Engineering; D053858:Metabolic Networks and Pathways / Q000235:genetics; D049490:Systems Biology
chemical_list: D002072:Butylene Glycols; D002247:Carbon Isotopes; C039681:1,4-butanediol