title: Oxyresveratrol suppresses lipopolysaccharide-induced inflammatory responses in murine macrophages.
abstract: Excessive inflammation is considered a critical factor in many human diseases. Oxyresveratrol(trans-2,3',4,5'-tetrahydroxystilbene), a natural hydroxystilbene, has been shown to possess antioxidant and free radical-scavenging activity. In this study, we investigated the effects of oxyresveratrol (OxyR) on the lipopolysaccharide (LPS)-induced production of inflammatory cytokines and mediators and further explored the mechanism of action in RAW264.7 murine macrophage cell line. Production of nitric oxide (NO), prostaglandin E2 (PGE2), messenger RNA (mRNA) and protein expressions of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), interleukin 6 (IL-6), and granulocyte macrophage colony-stimulating factor (GM-CSF), phosphorylation of mitogen-activated protein kinases (MAPKs; extracellular signal-regulated kinase (ERK), c-Jun N-terminal kinase (JNK), p38), and the activation of nuclear factor κ-light chain enhancer of activated B cells (NFκB) with OxyR were assayed in LPS-stimulated RAW264.7 cells. OxyR inhibited the productions of NO, PGE2, IL-6, and GM-CSF significantly in LPS-stimulated RAW264.7 cells. OxyR suppressed mRNA and protein expressions of iNOS, COX-2, IL-6, and GM-CSF in LPS-stimulated RAW264.7 cells. OxyR suppressed the phosphorylation of Akt and JNK and p38 MAPKs and the translocation of NFκB p65 subunit into the nucleus. These results indicate that OxyR inhibits LPS-stimulated inflammatory responses though the blocking of MAPK and NFκB signaling pathway in macrophages, and suggest that OxyR possesses anti-inflammatory effects.
authors.affiliation: Department of Food Science and Nutrition, Dongseo University, Busan, Republic of Korea.
authors.forename: H S
authors.initials: HS
authors.lastname: Lee
authors.affiliation: Center for Efficacy Assessment and Development of Functional Food and Drugs, Hallym University, Chuncheon, Republic of Korea.
authors.forename: D H
authors.initials: DH
authors.lastname: Kim
authors.affiliation: Center for Efficacy Assessment and Development of Functional Food and Drugs, Hallym University, Chuncheon, Republic of Korea.
authors.forename: J E
authors.initials: JE
authors.lastname: Hong
authors.affiliation: Center for Efficacy Assessment and Development of Functional Food and Drugs, Hallym University, Chuncheon, Republic of Korea Department of Biochemistry, College of Medicine, Hallym University, Chuncheon, Republic of Korea.
authors.forename: J-Y
authors.initials: JY
authors.lastname: Lee
authors.affiliation: Center for Efficacy Assessment and Development of Functional Food and Drugs, Hallym University, Chuncheon, Republic of Korea n-myej4@hallym.ac.kr.
authors.forename: E J
authors.initials: EJ
authors.lastname: Kim
mesh_terms: D000818:Animals; D002460:Cell Line; D051546:Cyclooxygenase 2 / Q000378:metabolism; D015232:Dinoprostone / Q000096:biosynthesis; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000096:biosynthesis; D007249:Inflammation / Q000517:prevention & control*; D015850:Interleukin-6 / Q000096:biosynthesis; D008070:Lipopolysaccharides / Q000633:toxicity*; D008264:Macrophages / Q000187:drug effects* / Q000201:enzymology / Q000276:immunology; D051379:Mice; D009569:Nitric Oxide / Q000096:biosynthesis; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism; D010766:Phosphorylation; D010936:Plant Extracts / Q000494:pharmacology*; D011494:Protein Kinases / Q000378:metabolism; D013267:Stilbenes / Q000494:pharmacology*
chemical_list: D015850:Interleukin-6; D008070:Lipopolysaccharides; D010936:Plant Extracts; D013267:Stilbenes; D009569:Nitric Oxide; C034912:puag-haad; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D052247:Nitric Oxide Synthase Type II; D051546:Cyclooxygenase 2; D011494:Protein Kinases; D015232:Dinoprostone
keywords: Akt; NFκB; Oxyresveratrol; anti-inflammation; granulocyte macrophage-colony stimulating factor; mitogen-activated protein kinase