title: The regulatory role of nickel on H3K27 demethylase JMJD3 in kidney cancer cells.
abstract: Nickel compounds are an important class of environmental pollutants and carcinogens. Chronic exposure to nickel compounds has been connected with increased risks of numerous cancers, including lung and kidney cancers. But the precise mechanism by which nickel compounds exert their carcinogenic properties is not completely understood. In this study, kidney cancer cells namely human embryonic kidney 293-containing SV40 large T-antigen (HEK293T) and 786-0 were incubated with various concentrations of nickel chloride for 24 h before analysing the expression of three histone H3K27 methylation-modifying enzymes and H3K27me3 using quantitative real-time polymerase chain reaction, Western blot and immunofluorescence analyses. Our results showed that incubation of nickel chloride upregulated the expression of H3K27me3 demethylase jumonji domain-containing protein 3 (JMJD3) in kidney cancer cells, which was accompanied by the reduction in the protein level of H3K27me3. Enhanced demethylation of H3K27me3 may represent a novel mechanism underlying the carcinogenicity of nickel compounds.
authors.affiliation: Shenzhen Key Laboratory of Genitourinary Tumor, Shenzhen Second People's Hospital, Shenzhen, China Laboratory of Molecular Iron Metabolism, College of Life Science, Hebei Normal University, Shijiazhuang, Hebei, China Department of Biochemistry, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, China.
authors.forename: Xiaoqiang
authors.initials: X
authors.lastname: Guo
authors.affiliation: Guangdong and Shenzhen Key Laboratory of Male Reproductive Medicine and Genetics, Institute of Urology, Peking University Shenzhen Hospital, Shenzhen PKU-HKUST Medical Center, Shenzhen, China.
authors.forename: Yanmin
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Guangdong and Shenzhen Key Laboratory of Male Reproductive Medicine and Genetics, Institute of Urology, Peking University Shenzhen Hospital, Shenzhen PKU-HKUST Medical Center, Shenzhen, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: Guangdong and Shenzhen Key Laboratory of Male Reproductive Medicine and Genetics, Institute of Urology, Peking University Shenzhen Hospital, Shenzhen PKU-HKUST Medical Center, Shenzhen, China.
authors.forename: Pingping
authors.initials: P
authors.lastname: Fa
authors.affiliation: Guangdong and Shenzhen Key Laboratory of Male Reproductive Medicine and Genetics, Institute of Urology, Peking University Shenzhen Hospital, Shenzhen PKU-HKUST Medical Center, Shenzhen, China.
authors.forename: Yaoting
authors.initials: Y
authors.lastname: Gui
authors.affiliation: Department of Biochemistry, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, China gaogq@mail.sysu.edu.cn.
authors.forename: Guoquan
authors.initials: G
authors.lastname: Gao
authors.affiliation: Shenzhen Key Laboratory of Genitourinary Tumor, Shenzhen Second People's Hospital, Shenzhen, China.
authors.forename: Zhiming
authors.initials: Z
authors.lastname: Cai
mesh_terms: D057809:HEK293 Cells; D006657:Histones / Q000378:metabolism; D006801:Humans; D056484:Jumonji Domain-Containing Histone Demethylases / Q000235:genetics / Q000378:metabolism*; D007668:Kidney / Q000166:cytology / Q000187:drug effects* / Q000473:pathology; D007680:Kidney Neoplasms; D008745:Methylation; D009532:Nickel / Q000633:toxicity*; D015854:Up-Regulation
chemical_list: D006657:Histones; C022838:nickel chloride; D009532:Nickel; D056484:Jumonji Domain-Containing Histone Demethylases; C524220:KDM6B protein, human
keywords: H3K27 demethylase; JMJD3; Nickel chloride; kidney cancer cell