title: Interleukin-17A and interleukin-17F gene polymorphisms and hepatitis B virus-related hepatocellular carcinoma risk in a Chinese population.
abstract: Interleukin (IL)-17A and IL-17F are inflammatory cytokines, which play a critical function in inflammation. Genetic variations in the IL-17A and IL-17F genes may be associated with a risk of hepatitis B virus (HBV)-related hepatocellular carcinoma (HCC), which is a typical inflammation-related cancer. However, their relationship with HBV-related HCC has not been thoroughly investigated. We conducted a case-control study including 155 patients with HBV-related HCC and 171 healthy controls to assess the association between IL-17A rs4711998, IL-17A rs2275913, and IL-17F rs763780 polymorphisms and risk of HCC. Genotypes were determined by polymerase chain reaction-restriction fragment length polymorphism and DNA sequencing. There were no significant differences in the genotype and allele frequencies of IL-17A rs4711998, IL-17A rs2275913, and IL-17F rs763780 polymorphisms between the HBV-related HCC patients and healthy controls. However, our results revealed a statistically significant association between the ACA haplotype and increased HCC risk [odds ratio (OR) 1.820, 95 % confidence interval (CI) 1.181-2.624, P = 0.013]. In contrast, the GCG haplotype was associated with a significantly decreased risk of HBV-related HCC (OR 0.454, 95 % CI 0.112-0.898, P = 0.035). Our results suggest that IL-17A rs4711998, IL-17A rs2275913, and IL-17F rs763780 polymorphisms do not contribute to HBV-related HCC susceptibility independently. However, the ACA and GCG haplotypes in the IL-17 gene might be a risk factor and a protective marker, respectively, for HBV-related HCC in a Chinese population.
authors.affiliation: Department of Clinical Laboratory, First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, China.
authors.forename: Xue-E
authors.initials: XE
authors.lastname: Xi
authors.forename: Yanqiong
authors.initials: Y
authors.lastname: Liu
authors.forename: Yu
authors.initials: Y
authors.lastname: Lu
authors.forename: Liying
authors.initials: L
authors.lastname: Huang
authors.forename: Xue
authors.initials: X
authors.lastname: Qin
authors.forename: Shan
authors.initials: S
authors.lastname: Li
mesh_terms: D044466:Asian People / Q000235:genetics*; D006528:Carcinoma, Hepatocellular / Q000235:genetics* / Q000821:virology; D016022:Case-Control Studies; D005260:Female; D020022:Genetic Predisposition to Disease / Q000235:genetics*; D005838:Genotype; D006509:Hepatitis B / Q000150:complications; D006801:Humans; D020381:Interleukin-17 / Q000235:genetics*; D008113:Liver Neoplasms / Q000235:genetics* / Q000821:virology; D008297:Male; D008875:Middle Aged; D012150:Polymorphism, Restriction Fragment Length; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012307:Risk Factors
chemical_list: C508717:IL17A protein, human; C440021:IL17F protein, human; D020381:Interleukin-17