title: Comparative studies on detergent-assisted apocytochrome b6 reconstitution into liposomal bilayers monitored by Zetasizer instruments.
abstract: The present paper is a systematic, comparative study on the reconstitution of an apocytochrome b6 purified from a heterologous system using a detergent-free method and reconstitution into liposomes performed using three different detergents: SDS, Triton X-100 and DM, and two methods of detergent removal by dialysis and using Bio-Beads. The product size, its distribution and zeta potential, and other parameters were monitored throughout the process. We found that zeta potential of proteoliposomes is correlated with reconstitution efficiency and, as such, can serve as a quick and convenient quality control for reconstitution experiments. We also advocate using detergent-free protein purification methods as they allow for an unfettered choice of detergent for reconstitution, which is the most crucial factor influencing the final product parameters.
authors.affiliation: Faculty of Biotechnology, University of Wroclaw, Wroclaw, Poland.
authors.forename: Michał A
authors.initials: MA
authors.lastname: Surma
authors.affiliation: Faculty of Biotechnology, University of Wroclaw, Wroclaw, Poland.
authors.forename: Andrzej
authors.initials: A
authors.lastname: Szczepaniak
authors.affiliation: Faculty of Biotechnology, University of Wroclaw, Wroclaw, Poland.
authors.forename: Jarosław
authors.initials: J
authors.lastname: Króliczewski
mesh_terms: D045347:Cytochromes b6 / Q000737:chemistry*; D003902:Detergents / Q000737:chemistry; D008051:Lipid Bilayers / Q000737:chemistry*; D008081:Liposomes / Q000737:chemistry*; D008564:Membrane Potentials
chemical_list: D045347:Cytochromes b6; D003902:Detergents; D008051:Lipid Bilayers; D008081:Liposomes