title: Early second-trimester serum microRNAs as potential biomarker for nondiabetic macrosomia.
abstract: BACKGROUND
  Macrosomia has become a worldwide problem with the rapid economic growth in the past few years. However, the detailed mechanism of how the macrosomia happened remains unknown. Growing evidence indicates that miRNAs are involved in maintaining metabolic homeostasis. We hypothesized that serum miRNAs are potential biomarkers for macrosomia.
  
  
  METHODS
  We performed miRNAs profiling using TLDA chips in the discovery phase in two pooled samples from 30 cases and 30 controls, respectively. Individual qRT-PCR was conducted for the discovery phase samples. To confirm the results, we detected the miRNAs which were differentially expressed in the microarray assays and individual qRT-PCR in external validation phase with another 30 cases and 30 controls.
  
  
  RESULTS
  In the discovery stage, miR-194 and miR-376a expression levels were significantly different between macrosomia group and controls (P=0.048 for miR-194 and P=0.018 for miR-376a, resp.). Further evaluation of the two miRNAs on a total of 120 serum samples showed that the miR-376a remains significantly lower in macrosomia (P=0.032). Receiver operating characteristic curve analyses showed that the area under curve for miR-376a was 67.8% (sensitivity=96.7% and specificity=40.0%).
  
  
  CONCLUSIONS
  Serum miR-376a may serve as a potential noninvasive biomarker in detecting macrosomia.
authors.affiliation: Changzhou Maternity and Child Health Care Hospital, Nanjing Medical University, Changzhou, Jiangsu 213003, China.
authors.forename: Lingmin
authors.initials: L
authors.lastname: Hu
authors.affiliation: Department of Epidemiology and Biostatistics, State Key Lab of Reproductive Medicine, School of Public Health, Nanjing Medical University, Nanjing 210029, China.
authors.forename: Jing
authors.identifier: 0000-0003-1754-6782
authors.initials: J
authors.lastname: Han
authors.affiliation: Changzhou Maternity and Child Health Care Hospital, Nanjing Medical University, Changzhou, Jiangsu 213003, China.
authors.forename: Fangxiu
authors.initials: F
authors.lastname: Zheng
authors.affiliation: Department of Epidemiology and Biostatistics, State Key Lab of Reproductive Medicine, School of Public Health, Nanjing Medical University, Nanjing 210029, China.
authors.forename: Hongxia
authors.initials: H
authors.lastname: Ma
authors.affiliation: Department of Epidemiology and Biostatistics, State Key Lab of Reproductive Medicine, School of Public Health, Nanjing Medical University, Nanjing 210029, China.
authors.forename: Jiaping
authors.identifier: 0000-0002-1687-8112
authors.initials: J
authors.lastname: Chen
authors.affiliation: Department of Epidemiology and Biostatistics, State Key Lab of Reproductive Medicine, School of Public Health, Nanjing Medical University, Nanjing 210029, China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Jiang
authors.affiliation: Changzhou Maternity and Child Health Care Hospital, Nanjing Medical University, Changzhou, Jiangsu 213003, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Jiang
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000097:blood*; D005260:Female; D005320:Fetal Macrosomia / Q000097:blood / Q000235:genetics* / Q000473:pathology; D005786:Gene Expression Regulation; D006801:Humans; D035683:MicroRNAs / Q000096:biosynthesis / Q000097:blood*; D011247:Pregnancy; D011262:Pregnancy Trimester, Second / Q000097:blood
chemical_list: D015415:Biomarkers; C554846:MIRN194 microRNA, human; C573498:MIRN376C microRNA, human; D035683:MicroRNAs