title: Comparative serology techniques for the diagnosis of Trypanosoma cruzi infection in a rural population from the state of Querétaro, Mexico.
abstract: Immunological diagnostic methods for Trypanosoma cruzi depend specifically on the presence of antibodies and parasitological methods lack sensitivity during the chronic and "indeterminate" stages of the disease. This study performed a serological survey of 1,033 subjects from 52 rural communities in 12 of the 18 municipalities in the state of Querétaro, Mexico. We detected anti-T. cruzi antibodies using the following tests: indirect haemagglutination assay (IHA), indirect immunofluorescence assay (IFA), ELISA and recombinant ELISA (rELISA). We also performed Western blot (WB) analysis using iron superoxide dismutase (FeSOD), a detoxifying enzyme excreted by the parasite, as the antigen. Positive test results were distributed as follows: ELISA 8%, rELISA 6.2%, IFA and IHA 5.4% in both cases and FeSOD 8%. A comparative study of the five tests was undertaken. Sensitivity levels, specificity, positive and negative predictive values, concordance percentage and kappa index were considered. Living with animals, trips to other communities, gender, age, type of housing and symptomatology at the time of the survey were statistically analysed using SPSS software v.11.5. Detection of the FeSOD enzyme that was secreted by the parasite and used as an antigenic fraction in WBs showed a 100% correlation with traditional ELISA tests.
authors.affiliation: Departamento de Investigación Biomédica, Facultad de Medicina, Universidad Autónoma de Querétaro, Santiago de Querétaro, Querétaro, México.
authors.forename: María Elena
authors.initials: ME
authors.lastname: Villagrán-Herrera
authors.affiliation: Departamento de Parasitología, Facultad de Ciencias, Universidad de Granada, Granada, España.
authors.forename: Manuel
authors.initials: M
authors.lastname: Sánchez-Moreno
authors.affiliation: Departamento de Investigación Biomédica, Facultad de Medicina, Universidad Autónoma de Querétaro, Santiago de Querétaro, Querétaro, México.
authors.forename: Adriana Jheny
authors.initials: AJ
authors.lastname: Rodríguez-Méndez
authors.affiliation: Departamento de Investigación Biomédica, Facultad de Medicina, Universidad Autónoma de Querétaro, Santiago de Querétaro, Querétaro, México.
authors.forename: Hebert Luis
authors.initials: HL
authors.lastname: Hernández-Montiel
authors.affiliation: Departamento de Investigación Biomédica, Facultad de Medicina, Universidad Autónoma de Querétaro, Santiago de Querétaro, Querétaro, México.
authors.forename: Felipe de Jesús
authors.initials: Fde J
authors.lastname: Dávila-Esquivel
authors.affiliation: Departamento de Investigación Biomédica, Facultad de Medicina, Universidad Autónoma de Querétaro, Santiago de Querétaro, Querétaro, México.
authors.forename: Germán
authors.initials: G
authors.lastname: González-Pérez
authors.affiliation: Centro Universitario del Sur, Universidad de Guadalajara, Ciudad Guzmán, Jalisco, México.
authors.forename: José Alejandro
authors.initials: JA
authors.lastname: Martínez-Ibarra
authors.affiliation: Departamento de Medicina Preventiva y Salud Pública, Facultad de Medicina, Universidad Autónoma de Madrid, Madrid, España.
authors.forename: José Antonio de
authors.initials: JA
authors.lastname: Diego-Cabrera
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000913:Antibodies, Protozoan / Q000302:isolation & purification*; D014355:Chagas Disease / Q000097:blood* / Q000175:diagnosis* / Q000453:epidemiology; D002648:Child; D002675:Child, Preschool; D006798:Housing; D006801:Humans; D007223:Infant; D008019:Life Style; D008800:Mexico / Q000453:epidemiology; D008875:Middle Aged; D012424:Rural Population; D016036:Seroepidemiologic Studies; D013482:Superoxide Dismutase / Q000378:metabolism; D014349:Trypanosoma cruzi / Q000201:enzymology / Q000276:immunology* / Q000302:isolation & purification; D055815:Young Adult
chemical_list: D000913:Antibodies, Protozoan; D013482:Superoxide Dismutase