title: [Expression of T lymphocyte cytokines in peripheral blood of renal transplant recipients].
abstract: OBJECTIVE
  To evaluate the clinical values of T-lymphocyte cytokines in renal transplant acute rejection.
  
  
  METHODS
  A total of 31 recipients with renal transplantation and 15 healthy volunteers from January 2010 to January 2012 were enrolled and divided into acute rejection group (n = 11) and stable renal allograft function group (n = 20) according to the inclusion criteria. Peripheral blood was collected from the patients before transplantation, 1, 7, 14, 28 day after transplantation and acute rejection onset. Cytometric bead array (CBA) was used to monitor the levels of interleukin-17 (IL-17), interferon-gamma (IFN-γ), tumor necrosis factor (TNF), interleukin(IL)-10, IL-6, IL-4 and IL-2. The associations of the changes and levels of cytokines in 3 groups were examined with Pearson correlation analysis.
  
  
  RESULTS
  The levels of IL-17A, TNF, IL-10 and IL-2 in recipients before transplantation were (3.40 ± 1.29) , (1.79 ± 0.53) , (2.73 ± 0.65) and (1.79 ± 1.02) ng/L respectively and decreased significantly compared to healthy volunteers ((4.52 ± 2.01), (3.36 ± 1.09) , (3.91 ± 0.42) , (3.12 ± 1.07) ng/L respectively, all P < 0.05). However the levels of IFN-γ, IL-6 and IL-4 showed no significant changes between two groups (all P > 0.05). In acute rejection group after transplantation, the levels of IL-17A, IFN-γ, IL-10 and IL-6 were (9.47 ± 4.75) , (5.01 ± 2.23) , (12.20 ± 5.79) , (6.55 ± 3.45) ng/L respectively and increased significantly compared to the renal allograft function group ((4.39 ± 1.26), (2.90 ± 0.87),(5.68 ± 2.25) and (2.10 ± 0.70) ng/L respectively, all P < 0.05); the level of IL-17A was correlated with those of IFN-γ and IL-4 (Pearson r = 0.519, 0.395, both P < 0.01), the level of IFN-γ was correlated with those of IL-4 and IL-2 (r = 0.276, 0.335, all P < 0.05) , the level of TNF was correlated with that of IL-4 (r = 0.423, P < 0.05) and the level of IL-10 was correlated with that of IL-6 (r = 0.361, P < 0.05).
  
  
  CONCLUSIONS
  Cytokines play an important role in acute rejection of renal transplant. And further understanding of its mechanism may provide experimental and preventive rationales.
authors.affiliation: Beijing Key Laboratory of Immunological Regulation and Organ Transplantation, Basic Research Laboratory of Organ Transplant Institute, 309th Hospital of PLA, Beijing 100091, China.
authors.forename: Li
authors.initials: L
authors.lastname: Xiao
authors.affiliation: Beijing Key Laboratory of Immunological Regulation and Organ Transplantation, Basic Research Laboratory of Organ Transplant Institute, 309th Hospital of PLA, Beijing 100091, China.
authors.forename: Xiuyun
authors.initials: X
authors.lastname: He
authors.affiliation: Beijing Key Laboratory of Immunological Regulation and Organ Transplantation, Basic Research Laboratory of Organ Transplant Institute, 309th Hospital of PLA, Beijing 100091, China.
authors.forename: Wenqiang
authors.initials: W
authors.lastname: Zhou
authors.affiliation: Beijing Key Laboratory of Immunological Regulation and Organ Transplantation, Basic Research Laboratory of Organ Transplant Institute, 309th Hospital of PLA, Beijing 100091, China.
authors.forename: Wenmei
authors.initials: W
authors.lastname: Fan
authors.affiliation: Beijing Key Laboratory of Immunological Regulation and Organ Transplantation, Basic Research Laboratory of Organ Transplant Institute, 309th Hospital of PLA, Beijing 100091, China.
authors.forename: Yuxiang
authors.initials: Y
authors.lastname: Wei
authors.affiliation: Beijing Key Laboratory of Immunological Regulation and Organ Transplantation, Basic Research Laboratory of Organ Transplant Institute, 309th Hospital of PLA, Beijing 100091, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Gao
authors.affiliation: Beijing Key Laboratory of Immunological Regulation and Organ Transplantation, Basic Research Laboratory of Organ Transplant Institute, 309th Hospital of PLA, Beijing 100091, China.
authors.forename: Xihui
authors.initials: X
authors.lastname: Ma
authors.affiliation: Beijing Key Laboratory of Immunological Regulation and Organ Transplantation, Basic Research Laboratory of Organ Transplant Institute, 309th Hospital of PLA, Beijing 100091, China.
authors.forename: Xiangrui
authors.initials: X
authors.lastname: Kong
authors.affiliation: Beijing Key Laboratory of Immunological Regulation and Organ Transplantation, Basic Research Laboratory of Organ Transplant Institute, 309th Hospital of PLA, Beijing 100091, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Han
authors.affiliation: Beijing Key Laboratory of Immunological Regulation and Organ Transplantation, Basic Research Laboratory of Organ Transplant Institute, 309th Hospital of PLA, Beijing 100091, China.
authors.forename: Xiaoguang
authors.initials: X
authors.lastname: Xu
authors.affiliation: Beijing Key Laboratory of Immunological Regulation and Organ Transplantation, Basic Research Laboratory of Organ Transplant Institute, 309th Hospital of PLA, Beijing 100091, China.
authors.forename: Haiyan
authors.initials: H
authors.lastname: Huang
authors.affiliation: Beijing Key Laboratory of Immunological Regulation and Organ Transplantation, Basic Research Laboratory of Organ Transplant Institute, 309th Hospital of PLA, Beijing 100091, China.
authors.forename: Xinying
authors.initials: X
authors.lastname: Wang
authors.affiliation: Beijing Key Laboratory of Immunological Regulation and Organ Transplantation, Basic Research Laboratory of Organ Transplant Institute, 309th Hospital of PLA, Beijing 100091, China. Email: shibingyi@medmail.com.cn.
authors.forename: Bingyi
authors.initials: B
authors.lastname: Shi
mesh_terms: D016207:Cytokines; D006801:Humans; D016030:Kidney Transplantation; D013601:T-Lymphocytes; D066027:Transplant Recipients; D014184:Transplantation, Homologous
chemical_list: D016207:Cytokines