title: Carbon dioxide stimulation of photosynthesis in Liquidambar styraciflua is not sustained during a 12-year field experiment.
abstract: Elevated atmospheric CO2 (eCO2) often increases photosynthetic CO2 assimilation (A) in field studies of temperate tree species. However, there is evidence that A may decline through time due to biochemical and morphological acclimation, and environmental constraints. Indeed, at the free-air CO2 enrichment (FACE) study in Oak Ridge, Tennessee, A was increased in 12-year-old sweetgum trees following 2 years of ∼40 % enhancement of CO2. A was re-assessed a decade later to determine if the initial enhancement of photosynthesis by eCO2 was sustained through time. Measurements were conducted at prevailing CO2 and temperature on detached, re-hydrated branches using a portable gas exchange system. Photosynthetic CO2 response curves (A versus the CO2 concentration in the intercellular air space (Ci); or A-Ci curves) were contrasted with earlier measurements using leaf photosynthesis model equations. Relationships between light-saturated photosynthesis (Asat), maximum electron transport rate (Jmax), maximum Rubisco activity (Vcmax), chlorophyll content and foliar nitrogen (N) were assessed. In 1999, Asat for eCO2 treatments was 15.4 ± 0.8 μmol m(-2) s(-1), 22 % higher than aCO2 treatments (P < 0.01). By 2009, Asat declined to <50 % of 1999 values, and there was no longer a significant effect of eCO2 (Asat = 6.9 or 5.7 ± 0.7 μmol m(-2) s(-1) for eCO2 or aCO2, respectively). In 1999, there was no treatment effect on area-based foliar N; however, by 2008, N content in eCO2 foliage was 17 % less than that in aCO2 foliage. Photosynthetic N-use efficiency (Asat : N) was greater in eCO2 in 1999 resulting in greater Asat despite similar N content, but the enhanced efficiency in eCO2 trees was lost as foliar N declined to sub-optimal levels. There was no treatment difference in the declining linear relationships between Jmax or Vcmax with declining N, or in the ratio of Jmax : Vcmax through time. Results suggest that the initial enhancement of photosynthesis to elevated CO2 will not be sustained through time if N becomes limited.
authors.affiliation: Climate Change Science Institute and Environmental Sciences Division, Oak Ridge National Laboratory, Oak Ridge, TN 37831-6301, USA warrenjm@ornl.gov.
authors.forename: Jeffrey M
authors.initials: JM
authors.lastname: Warren
authors.affiliation: Climate Change Science Institute and Environmental Sciences Division, Oak Ridge National Laboratory, Oak Ridge, TN 37831-6301, USA.
authors.forename: Anna M
authors.initials: AM
authors.lastname: Jensen
authors.affiliation: School of Biological Sciences, Macquarie University, Sydney, NSW 2019, Australia.
authors.forename: Belinda E
authors.initials: BE
authors.lastname: Medlyn
authors.affiliation: Climate Change Science Institute and Environmental Sciences Division, Oak Ridge National Laboratory, Oak Ridge, TN 37831-6301, USA.
authors.forename: Richard J
authors.initials: RJ
authors.lastname: Norby
authors.affiliation: Hawkesbury Institute for the Environment, University of Western Sydney, Richmond, NSW 2753, Australia.
authors.forename: David T
authors.initials: DT
authors.lastname: Tissue
keywords: Acclimation; down-regulation; free-air CO2 enrichment; nitrogen limitation; sweetgum.