title: Cherry.
abstract: Agrobacterium tumefaciens-mediated transformation of sour chgahtvy (Prunus cerasus L.) "Montmorency" and sweet cherry rootstocks "Gisela 6" and "Gisela 7" (P. cerasus × P. canescens) is described. Briefly, leaf explants from in vitro shoots are cocultivated with A. tumefaciens either directly (for "Gisela 6" and "Gisela 7") or after pretreatment (for "Montmorency") on cocultivation medium; selection and regeneration of transformed shoots are carried out on selection medium containing 50 mg/L kanamycin (Km) and 250 mg/L timentin (or cefotaxime) for 3-5 months. In this protocol, the optimal media for shoot proliferation and shoot regeneration from leaf explants are genotype dependent.
authors.affiliation: Department of Horticulture, Plant Biotechnology Resource and Outreach Center, Michigan State University, 1066 Bogue Street, East Lansing, MI, 48824, USA, songg@msu.edu.
authors.forename: Guo-Qing
authors.initials: GQ
authors.lastname: Song
mesh_terms: D016960:Agrobacterium tumefaciens / Q000235:genetics / Q000254:growth & development; D018920:Coculture Techniques; D004780:Environment, Controlled; D005818:Genetic Engineering / Q000379:methods*; D018515:Plant Leaves / Q000254:growth & development; D018517:Plant Roots / Q000254:growth & development; D027861:Prunus / Q000235:genetics* / Q000254:growth & development* / Q000502:physiology; D012038:Regeneration; D014170:Transformation, Genetic