title: Reduced synaptic vesicle protein degradation at lysosomes curbs TBC1D24/sky-induced neurodegeneration.
abstract: Synaptic demise and accumulation of dysfunctional proteins are thought of as common features in neurodegeneration. However, the mechanisms by which synaptic proteins turn over remain elusive. In this paper, we study Drosophila melanogaster lacking active TBC1D24/Skywalker (Sky), a protein that in humans causes severe neurodegeneration, epilepsy, and DOOR (deafness, onychdystrophy, osteodystrophy, and mental retardation) syndrome, and identify endosome-to-lysosome trafficking as a mechanism for degradation of synaptic vesicle-associated proteins. In fly sky mutants, synaptic vesicles traveled excessively to endosomes. Using chimeric fluorescent timers, we show that synaptic vesicle-associated proteins were younger on average, suggesting that older proteins are more efficiently degraded. Using a genetic screen, we find that reducing endosomal-to-lysosomal trafficking, controlled by the homotypic fusion and vacuole protein sorting (HOPS) complex, rescued the neurotransmission and neurodegeneration defects in sky mutants. Consistently, synaptic vesicle proteins were older in HOPS complex mutants, and these mutants also showed reduced neurotransmission. Our findings define a mechanism in which synaptic transmission is facilitated by efficient protein turnover at lysosomes and identify a potential strategy to suppress defects arising from TBC1D24 mutations in humans.
authors.affiliation: VIB Center for the Biology of Disease and Center for Human Genetics Laboratory of Neuronal Communication, KU Leuven, 3000 Leuven, Belgium VIB Center for the Biology of Disease and Center for Human Genetics Laboratory of Neuronal Communication, KU Leuven, 3000 Leuven, Belgium.
authors.forename: Ana Clara
authors.initials: AC
authors.lastname: Fernandes
authors.affiliation: VIB Center for the Biology of Disease and Center for Human Genetics Laboratory of Neuronal Communication, KU Leuven, 3000 Leuven, Belgium VIB Center for the Biology of Disease and Center for Human Genetics Laboratory of Neuronal Communication, KU Leuven, 3000 Leuven, Belgium.
authors.forename: Valerie
authors.initials: V
authors.lastname: Uytterhoeven
authors.affiliation: VIB Center for the Biology of Disease and Center for Human Genetics Laboratory of Neuronal Communication, KU Leuven, 3000 Leuven, Belgium VIB Center for the Biology of Disease and Center for Human Genetics Laboratory of Neuronal Communication, KU Leuven, 3000 Leuven, Belgium.
authors.forename: Sabine
authors.initials: S
authors.lastname: Kuenen
authors.affiliation: VIB Center for the Biology of Disease and Center for Human Genetics Laboratory of Neuronal Communication, KU Leuven, 3000 Leuven, Belgium VIB Center for the Biology of Disease and Center for Human Genetics Laboratory of Neuronal Communication, KU Leuven, 3000 Leuven, Belgium.
authors.forename: Yu-Chun
authors.initials: YC
authors.lastname: Wang
authors.affiliation: VIB Center for the Biology of Disease and Center for Human Genetics Laboratory of Neuronal Communication, KU Leuven, 3000 Leuven, Belgium VIB Center for the Biology of Disease and Center for Human Genetics Laboratory of Neuronal Communication, KU Leuven, 3000 Leuven, Belgium.
authors.forename: Jan R
authors.initials: JR
authors.lastname: Slabbaert
authors.affiliation: VIB Center for the Biology of Disease and Center for Human Genetics Laboratory of Neuronal Communication, KU Leuven, 3000 Leuven, Belgium VIB Center for the Biology of Disease and Center for Human Genetics Laboratory of Neuronal Communication, KU Leuven, 3000 Leuven, Belgium.
authors.forename: Jef
authors.initials: J
authors.lastname: Swerts
authors.affiliation: VIB Center for the Biology of Disease and Center for Human Genetics Laboratory of Neuronal Communication, KU Leuven, 3000 Leuven, Belgium VIB Center for the Biology of Disease and Center for Human Genetics Laboratory of Neuronal Communication, KU Leuven, 3000 Leuven, Belgium.
authors.forename: Jaroslaw
authors.initials: J
authors.lastname: Kasprowicz
authors.affiliation: VIB Center for the Biology of Disease and Center for Human Genetics Laboratory of Neuronal Communication, KU Leuven, 3000 Leuven, Belgium.
authors.forename: Stein
authors.initials: S
authors.lastname: Aerts
authors.affiliation: VIB Center for the Biology of Disease and Center for Human Genetics Laboratory of Neuronal Communication, KU Leuven, 3000 Leuven, Belgium VIB Center for the Biology of Disease and Center for Human Genetics Laboratory of Neuronal Communication, KU Leuven, 3000 Leuven, Belgium patrik.verstreken@med.kuleuven.be.
authors.forename: Patrik
authors.initials: P
authors.lastname: Verstreken
mesh_terms: D000818:Animals; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism; D019465:Craniofacial Abnormalities / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D029721:Drosophila Proteins / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000235:genetics / Q000378:metabolism*; D011992:Endosomes / Q000378:metabolism; D020690:GTPase-Activating Proteins; D006228:Hand Deformities, Congenital / Q000235:genetics; D006319:Hearing Loss, Sensorineural / Q000235:genetics; D006801:Humans; D008607:Intellectual Disability / Q000235:genetics; D008247:Lysosomes / Q000378:metabolism; D008562:Membrane Glycoproteins / Q000378:metabolism; D008565:Membrane Proteins; D009154:Mutation; D009264:Nails, Malformed / Q000235:genetics; D009419:Nerve Tissue Proteins; D019636:Neurodegenerative Diseases / Q000235:genetics / Q000378:metabolism*; D009469:Neuromuscular Junction / Q000378:metabolism; D021381:Protein Transport; D059748:Proteolysis; D011726:Pyridinium Compounds / Q000494:pharmacology; D000644:Quaternary Ammonium Compounds / Q000494:pharmacology; D050683:R-SNARE Proteins / Q000096:biosynthesis / Q000235:genetics; D009435:Synaptic Transmission / Q000502:physiology*; D013572:Synaptic Vesicles / Q000378:metabolism; D020691:rab GTP-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D020696:rab5 GTP-Binding Proteins / Q000096:biosynthesis
chemical_list: D002352:Carrier Proteins; D004268:DNA-Binding Proteins; C104790:DOR protein, Drosophila; D029721:Drosophila Proteins; C073804:FM1 43; D020690:GTPase-Activating Proteins; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D011726:Pyridinium Compounds; D000644:Quaternary Ammonium Compounds; D050683:R-SNARE Proteins; C552551:TBC1D24 protein, human; C084068:nsyb protein, Drosophila; C558398:sky protein, Drosophila; D020691:rab GTP-Binding Proteins; D020696:rab5 GTP-Binding Proteins