title: Collagen labelling with an azide-proline chemical reporter in live cells.
abstract: We have developed a strategy for selective imaging of collagen in live foetal ovine osteoblasts. Our approach involves the incorporation of an azide-tagged proline in the biosynthesis of collagen followed by labelling using a strain-promoted [3+2] azide-alkyne cycloaddition reaction.
authors.affiliation: Department of Chemistry, University of Cambridge, Lensfield Road, CB2 1EW Cambridge, UK. gb453@cam.ac.uk.
authors.forename: Beatrice
authors.initials: B
authors.lastname: Amgarten
authors.forename: Rakesh
authors.initials: R
authors.lastname: Rajan
authors.forename: Nuria
authors.initials: N
authors.lastname: Martínez-Sáez
authors.forename: Bruno L
authors.initials: BL
authors.lastname: Oliveira
authors.forename: Inês S
authors.initials: IS
authors.lastname: Albuquerque
authors.forename: Roger A
authors.initials: RA
authors.lastname: Brooks
authors.forename: David G
authors.initials: DG
authors.lastname: Reid
authors.forename: Melinda J
authors.initials: MJ
authors.lastname: Duer
authors.forename: Gonçalo J L
authors.initials: GJ
authors.lastname: Bernardes
mesh_terms: D000480:Alkynes / Q000737:chemistry; D001386:Azides / Q000737:chemistry*; D002470:Cell Survival; D002478:Cells, Cultured; D003094:Collagen / Q000032:analysis*; D003500:Cyclization; D006801:Humans; D010006:Osteoblasts / Q000737:chemistry* / Q000166:cytology*; D011392:Proline / Q000737:chemistry*
chemical_list: D000480:Alkynes; D001386:Azides; D003094:Collagen; D011392:Proline