title: Report: Optimization study of the preparation factors for argan oil microcapsule based on hybrid-level orthogonal array design via SPSS modeling.
abstract: To optimize the preparation factors for argan oil microcapsule using complex coacervation of chitosan cross-linked with gelatin based on hybrid-level orthogonal array design via SPSS modeling. Eight relatively significant factors were firstly investigated and selected as calculative factors for the orthogonal array design from the total of ten factors effecting the preparation of argan oil microcapsule by utilizing the single factor variable method. The modeling of hybrid-level orthogonal array design was built in these eight factors with the relevant levels (9, 9, 9, 9, 7, 6, 2 and 2 respectively). The preparation factors for argan oil microcapsule were investigated and optimized according to the results of hybrid-level orthogonal array design. The priorities order and relevant optimum levels of preparation factors standard to base on the percentage of microcapsule with the diameter of 30~40 μm via SPSS. Experimental data showed that the optimum factors were controlling the chitosan/gelatin ratio, the systemic concentration and the core/shell ratio at 1:2, 1.5% and 1:7 respectively, presetting complex coacervation pH at 6.4, setting cross-linking time and complex coacervation at 75 min and 30 min, using the glucose-delta lactone as the type of cross-linking agent, and selecting chitosan with the molecular weight of 2000～3000.
authors.affiliation: Department of Chemistry and Pharmacy, Zhuhai College of Jilin University, Zhuhai, China.
authors.forename: Xi
authors.initials: X
authors.lastname: Zhao
authors.affiliation: Faculty of Health Sciences, University of Macau, Macau, China.
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Wu
authors.affiliation: Department of Chemistry and Pharmacy, Zhuhai College of Jilin University, Zhuhai, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Zhou
authors.affiliation: Department of Chemistry and Pharmacy, Zhuhai College of Jilin University, Zhuhai, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Jiang
authors.affiliation: College of Life Science and Technology, Jinan University, China.
authors.forename: Chun
authors.initials: C
authors.lastname: Chen
authors.affiliation: Department of Chemistry and Pharmacy, Zhuhai College of Jilin University, Zhuhai, China.
authors.forename: Mingshi
authors.initials: M
authors.lastname: Liu
authors.affiliation: Department of Chemistry and Pharmacy, Zhuhai College of Jilin University, Zhuhai, China.
authors.forename: Yuanbao
authors.initials: Y
authors.lastname: Jin
authors.affiliation: Department of Chemistry and Pharmacy, Zhuhai College of Jilin University, Zhuhai, China.
authors.forename: Dongsheng
authors.initials: D
authors.lastname: Yang
mesh_terms: D002214:Capsules; D006863:Hydrogen-Ion Concentration; D015233:Models, Statistical; D010938:Plant Oils / Q000008:administration & dosage*
chemical_list: D002214:Capsules; D010938:Plant Oils; C555574:argan oil