title: Molecular cloning and characterization of mannose binding lectin homologue from snow trout (Schizothorax richardsonii).
abstract: The teleost fishes depend on their innate immune system for first line of defense. Mannose binding lectin (MBL) is a member of the collectin family of proteins that plays an important role in the innate immune response in many vertebrates including fish. The present study was thus carried out to identify and characterize MBL homologue from snow trout. The cDNA of snow trout MBL homologue (galactose binding; Sch-GBL) was cloned and characterized. The obtained cDNA (778-bp) contains 762 bp long ORF (open reading frame) and a 16 bp partial 3'UTR (untranslated region); transcripts are ubiquitously expressed in all the tissues under study. Sch-GBL encodes a 253-aa protein that clusters with that of common carp, goldfish and contain several common structural motifs. In addition, the K(a)/K(s) ratios of the C-type lectin domain sequences depicts that they are under negative selection. Sch-GBL transcript level increases in response to challenge to Aeromonas hydrophila infection and decreases in response to downshift in temperature. Altogether, these findings suggest that Sch-GBL play an important role in the first line of fish defense mechanisms.
authors.affiliation: Molecular Genetics Laboratory, Directorate of Coldwater Fisheries Research, Indian Council of Agricultural Research, Bhimtal, Nainital, 263136, Uttarakhand, India.
authors.forename: Chirag
authors.initials: C
authors.lastname: Goel
authors.forename: Ashoktaru
authors.initials: A
authors.lastname: Barat
authors.forename: Veena
authors.initials: V
authors.lastname: Pande
authors.forename: Prabhati Kumari
authors.initials: PK
authors.lastname: Sahoo
mesh_terms: D016980:Aeromonas hydrophila; D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D005393:Fish Diseases / Q000235:genetics / Q000276:immunology; D029941:Fish Proteins / Q000235:genetics / Q000276:immunology; D016905:Gram-Negative Bacterial Infections / Q000235:genetics / Q000276:immunology / Q000662:veterinary; D007113:Immunity, Innate / Q000502:physiology*; D037601:Mannose-Binding Lectin / Q000235:genetics / Q000276:immunology; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D014337:Trout / Q000235:genetics / Q000276:immunology
chemical_list: D029941:Fish Proteins; D037601:Mannose-Binding Lectin