title: Downregulation of microRNA-100 protects apoptosis and promotes neuronal growth in retinal ganglion cells.
abstract: BACKGROUND
  Retinal ganglion cells (RGCs) are preferentially lost in glaucoma or optic neuritis. In the present study, we investigated the protective effect of mircoRNA 100 (miR-100) against oxidative stress induced apoptosis in RGC-5 cells.
  
  
  RESULTS
  Rat RGC-5 cells were cultured in plates and H2O2 was added to induce oxidative stress. TUNEL assay and qRT-PCR showed H2O2 induced apoptosis and up-regulated miR-100 in a dose-dependent manner in RGC-5 cells. Conversely, lentiviral-mediated miR-100 down-regulation protected H2O2 induced apoptosis, promoted neurite growth and activated AKT/ERK and TrkB pathways through phosphorylation. Luciferase assay confirmed that IGF1R was directly regulated by miR-100 in RGC-5 cells, and siRNA-mediated IGF1R knockdown activated AKT protein through phosphorylation, down-regulated miR-100, therefore exerted a protective effect on RGC-5 apoptosis.
  
  
  CONCLUSIONS
  Down-regulating miR-100 is an effective method to protect H2O2 induced apoptosis in RGC-5 cells, possible associated with IGF1R regulation.
authors.affiliation: Department of Ophthalmology, Zhujiang Hospital, Southern Medical University, Guangzhou, 510280, Guangdong Province, China. kn13@tom.com.|Department of Ophthalmology, Guangzhou Panyu Central Hospital, Guangzhou, 510280, Guangdong Province, China. kn13@tom.com.
authors.forename: Ning
authors.initials: N
authors.lastname: Kong
authors.affiliation: Department of Ophthalmology, Zhujiang Hospital, Southern Medical University, Guangzhou, 510280, Guangdong Province, China. libin_bin@aol.com.
authors.forename: Xiaohe
authors.initials: X
authors.lastname: Lu
authors.affiliation: Department of Ophthalmology, Guangzhou Panyu Central Hospital, Guangzhou, 510280, Guangdong Province, China. 23812147@qq.com.
authors.forename: Bin
authors.initials: B
authors.lastname: Li
mesh_terms: D000818:Animals; D017209:Apoptosis; D002460:Cell Line; D015536:Down-Regulation; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000494:pharmacology; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D018384:Oxidative Stress; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D034622:RNA Interference; D051381:Rats; D017526:Receptor, IGF Type 1 / Q000235:genetics / Q000378:metabolism; D020813:Receptor, trkB / Q000378:metabolism; D012165:Retinal Ganglion Cells / Q000502:physiology*; D015398:Signal Transduction
chemical_list: C577629:MIRN100 microRNA, rat; D035683:MicroRNAs; D006861:Hydrogen Peroxide; D017526:Receptor, IGF Type 1; D020813:Receptor, trkB; D051057:Proto-Oncogene Proteins c-akt; D048049:Extracellular Signal-Regulated MAP Kinases