title: Application of two-dimensional chromatography to the characterization of macromolecules and biomacromolecules.
abstract: Modern polymeric materials are heterogeneous with respect to different structural features, for instance molar mass, composition, and architecture. One-dimensional separation methods such as size-exclusion chromatography (SEC) are insufficient to fully resolve the multidimensional distributions of such complex materials. Therefore, two-dimensional separation methods are increasingly employed to characterize macromolecules. The present article describes in detail the advantages and experimental aspects of two-dimensional macromolecular separations. Selected examples will be discussed to explain the strategies used to separate macromolecules with respect to specific structural features.
authors.affiliation: PSS Polymer Standards Service GmbH, In der Dalheimer Wiese 5, 55120, Mainz, Germany.
authors.forename: Peter
authors.initials: P
authors.lastname: Kilz
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Radke
mesh_terms: D000818:Animals; D002853:Chromatography, Liquid / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D046911:Macromolecular Substances / Q000737:chemistry / Q000302:isolation & purification*
chemical_list: D046911:Macromolecular Substances