title: DHA concentration of red blood cells is inversely associated with markers of lipid peroxidation in men taking DHA supplement.
abstract: An increase in the proportion of fatty acids with higher numbers of double bonds is believed to increase lipid peroxidation, which augments the risk for many chronic diseases. (n-3) Polyunsaturated fatty acids provide various health benefits, but there is a concern that they might increase lipid peroxidation. We examined the effects of docosahexaenoic acid [22:6 (n-3)] supplementation on lipid peroxidation markers in plasma and red blood cells (RBC) and their associations with red blood cell and plasma fatty acids. Hypertriglyceridemic men (n = 17 per group) aged 39-66 years participated in a double-blind, randomized, placebo-controlled, parallel study. They received no supplements for the first 8 days and then received 7.5 g/day docosahexaenoic acid oil (3 g/day docosahexaenoic acid) or olive oil (placebo) for 90 days. Fasting blood samples were collected 0, 45, and 91 days after supplementation. Docosahexaenoic acid supplementation did not change plasma or RBC concentrations of lipid peroxidation markers (total hydroxyoctadecadienoic acid, total hydroxyeicosatetraenoic acid, total 8-isoprostaglandin F2α, 7α-hydroxycholesterol, 7β-hydroxycholesterol) when pre- and post-supplement values were compared. However, the post-supplement docosahexaenoic acid (DHA) concentration was inversely associated with RBC concentrations of ZE-HODE, EE-HODE, t-HODE, and total 8-isoprostaglandin F2α, (p<0.05). RBC concentration of hydroxycholesterol was also inversely associated with DHA but it did not attain significance (p = 0.07). Our results suggest that increased concentration of DHA in RBC lipids reduced lipid peroxidation. This may be another health benefit of DHA in addition to its many other health promoting effects.
authors.affiliation: Health Research Institute (HRI), National Institute of Advanced Industrial Science and Technology (AIST), 2217-14 Hayashi-cho, Takamatsu, Kagawa 761-0395, Japan.
authors.forename: Mototada
authors.initials: M
authors.lastname: Shichiri
authors.affiliation: Western Human Nutrition Research Center, ARS, USDA and Department of Nutrition, University of California Davis, CA 95616, USA.
authors.forename: Yuriko
authors.initials: Y
authors.lastname: Adkins
authors.affiliation: Health Research Institute (HRI), National Institute of Advanced Industrial Science and Technology (AIST), 2217-14 Hayashi-cho, Takamatsu, Kagawa 761-0395, Japan.
authors.forename: Noriko
authors.initials: N
authors.lastname: Ishida
authors.affiliation: Health Research Institute (HRI), National Institute of Advanced Industrial Science and Technology (AIST), 2217-14 Hayashi-cho, Takamatsu, Kagawa 761-0395, Japan.
authors.forename: Aya
authors.initials: A
authors.lastname: Umeno
authors.affiliation: Health Research Institute (HRI), National Institute of Advanced Industrial Science and Technology (AIST), 2217-14 Hayashi-cho, Takamatsu, Kagawa 761-0395, Japan.
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Shigeri
authors.affiliation: Health Research Institute (HRI), National Institute of Advanced Industrial Science and Technology (AIST), 2217-14 Hayashi-cho, Takamatsu, Kagawa 761-0395, Japan.
authors.forename: Yasukazu
authors.initials: Y
authors.lastname: Yoshida
authors.affiliation: Western Human Nutrition Research Center, ARS, USDA and Department of Nutrition, University of California Davis, CA 95616, USA.
authors.forename: Dawn M
authors.initials: DM
authors.lastname: Fedor
authors.affiliation: Western Regional Research Center, ARS, USDA, Albany, CA 94710, USA.
authors.forename: Bruce E
authors.initials: BE
authors.lastname: Mackey
authors.affiliation: Western Human Nutrition Research Center, ARS, USDA and Department of Nutrition, University of California Davis, CA 95616, USA.
authors.forename: Darshan S
authors.initials: DS
authors.lastname: Kelley
keywords: antioxidant; docosahexaenoic acid; hypertriglyceridemia; lipid peroxidation; polyunsaturated fatty acid