title: MiR-218-targeting-Bmi-1 mediates the suppressive effect of 1,6,7-trihydroxyxanthone on liver cancer cells.
abstract: Traditional Chinese medicine is recently emerged as anti-cancer therapy or adjuvant with reduced side-effects and improved quality of life. In the present study, an active ingredient, 1,6,7-trihydroxyxanthone (THA), derived from Goodyera oblongifolia was found to strongly suppress cell growth and induce apoptosis in liver cancer cells. MicroRNAs are a group of small non-coding RNAs that regulate gene expression at post-transcriptional levels. Our results demonstrated that miR-218 was up-regulated and oncogene Bmi-1 was down-regulated by THA treatment. Further investigation showed that THA-induced-miR-218 up-regulation could lead to activation of tumor suppressor P16(Ink4a) and P14(ARF), the main down-stream targets of Bmi-1. In conclusion, THA might be a potential anti-cancer drug candidate, at least in part, through the activation of miR-218 and suppression of Bmi-1 expression.
authors.affiliation: School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong, People's Republic of China.
authors.forename: Wei-Ming
authors.initials: WM
authors.lastname: Fu
authors.forename: Li-Peng
authors.initials: LP
authors.lastname: Tang
authors.forename: Xiao
authors.initials: X
authors.lastname: Zhu
authors.forename: Ying-Fei
authors.initials: YF
authors.lastname: Lu
authors.forename: Yan-Ling
authors.initials: YL
authors.lastname: Zhang
authors.forename: Wayne Yuk-Wai
authors.initials: WY
authors.lastname: Lee
authors.forename: Hua
authors.initials: H
authors.lastname: Wang
authors.forename: Yang
authors.initials: Y
authors.lastname: Yu
authors.forename: Wei-Cheng
authors.initials: WC
authors.lastname: Liang
authors.forename: Chun-Hay
authors.initials: CH
authors.lastname: Ko
authors.forename: Hong-Xi
authors.initials: HX
authors.lastname: Xu
authors.forename: Hsiang-Fu
authors.initials: HF
authors.lastname: Kung
authors.forename: Jin-Fang
authors.initials: JF
authors.lastname: Zhang
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D006801:Humans; D008113:Liver Neoplasms / Q000378:metabolism / Q000473:pathology / Q000628:therapy; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D029595:Orchidaceae / Q000737:chemistry*; D010936:Plant Extracts / Q000494:pharmacology; D063150:Polycomb Repressive Complex 1 / Q000378:metabolism*; D044004:Xanthones / Q000737:chemistry / Q000494:pharmacology*
chemical_list: C000600338:1,6,7-trihydroxyxanthone; D000970:Antineoplastic Agents; C069077:BMI1 protein, human; C547471:MIRN218 microRNA, human; D035683:MicroRNAs; D010936:Plant Extracts; D044004:Xanthones; D063150:Polycomb Repressive Complex 1