title: Therapeutic effect of Xue Niao An on glyoxylate-induced calcium oxalate crystal deposition based on urinary metabonomics approach.
abstract: The anti-nephrolithiasis effect of Xue Niao An (XNA) capsules is explored by analyzing urine metabolic profiles in mouse models, with ultra-high performance liquid chromatography quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF/MS). An animal model of calcium oxalate crystal renal deposition was established in mice by intra-abdominal injection of glyoxylate. Then, treatment with XNA by intra-gastric administration was performed. At the end of the study, calcium deposition in kidney was measured by Von Kossa staining under light microscopy, and the Von Kossa staining changes showed that XNA significantly alleviated the calcium oxalate crystal deposition. Meanwhile, urine samples for fifteen metabolites, including amino acids and fatty acids, with significant differences were detected in the calcium oxalate group, while XNA treatment attenuated metabolic imbalances. Our study indicated that the metabonomic strategy provided comprehensive insight on the metabolic response to XNA treatment of rodent renal calcium oxalate deposition.
authors.affiliation: Department of Nephrology, Changhai Hospital, Second Military Medical University, Shanghai 200433, China.
authors.forename: Zhongjiang
authors.initials: Z
authors.lastname: Peng
authors.affiliation: Department of Nephrology, Changhai Hospital, Second Military Medical University, Shanghai 200433, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Chen
authors.affiliation: School of Pharmacy, Second Military Medical University, Shanghai 200433, China.
authors.forename: Songyan
authors.initials: S
authors.lastname: Gao
authors.affiliation: School of Pharmacy, Second Military Medical University, Shanghai 200433, China.
authors.forename: Li
authors.initials: L
authors.lastname: Su
authors.affiliation: School of Pharmacy, Second Military Medical University, Shanghai 200433, China.
authors.forename: Na
authors.initials: N
authors.lastname: Li
authors.affiliation: Department of Nephrology, Changhai Hospital, Second Military Medical University, Shanghai 200433, China.
authors.forename: Li
authors.initials: L
authors.lastname: Wang
authors.affiliation: School of Pharmacy, Second Military Medical University, Shanghai 200433, China.
authors.forename: Ziyang
authors.initials: Z
authors.lastname: Lou
authors.affiliation: School of Pharmacy, Second Military Medical University, Shanghai 200433, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Dong
authors.affiliation: Department of Nephrology, Changhai Hospital, Second Military Medical University, Shanghai 200433, China.
authors.forename: Zhiyong
authors.initials: Z
authors.lastname: Guo
keywords: UPLC-Q-TOF/MS; Xue Niao An; calcium oxalate; metabonomics