title: [Production of β-carotene by metabolically engineered Saccharomyces cerevisiae].
abstract: β-carotene has a wide range of application in food, pharmaceutical and cosmetic industries. For microbial production of β-carotene in Saccharomyces cerevisiae, the supply of geranylgeranyl diphosphate (GGPP) was firstly increased in S. cerevisiae BY4742 to obtain strain BY4742-T2 through over-expressing truncated 3-hydroxy-3-methylglutaryl-CoA reductase (tHMGR), which is the major rate-limiting enzyme in the mevalonate (MVA) pathway, and GGPP synthase (GGPS), which is a key enzyme in the diterpenoid synthetic pathway. The β-carotene synthetic genes of Pantoea agglomerans and Xanthophyllomyces dendrorhous were further integrated into strain BY4742-T2 for comparing β-carotene production. Over-expression of tHMGR and GGPS genes led to 26.0-fold increase of β-carotene production. In addition, genes from X. dendrorhous was more efficient than those from P. agglomerans for β-carotene production in S. cerevisiae. Strain BW02 was obtained which produced 1.56 mg/g (dry cell weight) β-carotene, which could be used further for constructing cell factories for β-carotene production.
authors.forename: Beibei
authors.initials: B
authors.lastname: Wang
authors.forename: Mingyu
authors.initials: M
authors.lastname: Shi
authors.forename: Dong
authors.initials: D
authors.lastname: Wang
authors.forename: Jiaoyang
authors.initials: J
authors.lastname: Xu
authors.forename: Yi
authors.initials: Y
authors.lastname: Liu
authors.forename: Hongjiang
authors.initials: H
authors.lastname: Yang
authors.forename: Zhubo
authors.initials: Z
authors.lastname: Dai
authors.forename: Xueli
authors.initials: X
authors.lastname: Zhang
mesh_terms: D001487:Basidiomycota / Q000201:enzymology; D051231:Farnesyltranstransferase / Q000235:genetics / Q000378:metabolism; D006903:Hydroxymethylglutaryl CoA Reductases / Q000235:genetics / Q000378:metabolism; D060847:Metabolic Engineering; D011106:Polyisoprenyl Phosphates; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D019207:beta Carotene / Q000096:biosynthesis*
chemical_list: D011106:Polyisoprenyl Phosphates; D019207:beta Carotene; D006903:Hydroxymethylglutaryl CoA Reductases; D051231:Farnesyltranstransferase; C002963:geranylgeranyl pyrophosphate