title: The small molecule probe PT-Yellow labels the renal proximal tubules in zebrafish.
abstract: We report the development of a small fluorescent molecule, BDNCA3-D2, herein referred to as PT-Yellow. Soaking zebrafish embryos in PT-Yellow or intraperitoneal injection into adults results in non-toxic in vivo fluorescent labeling of the renal proximal tubules, the major site of blood filtrate reabsorption and a common target of injury in acute kidney injury. We demonstrate the applicability of this new compound as a rapid and simple readout for zebrafish kidney filtration and proximal tubule reabsorption function.
authors.affiliation: Department of Molecular Medicine & Pathology, The University of Auckland, Auckland 1142, New Zealand. a.davidson@auckland.ac.nz.
authors.forename: Veronika
authors.initials: V
authors.lastname: Sander
authors.forename: Shantanu
authors.initials: S
authors.lastname: Patke
authors.forename: Srikanta
authors.initials: S
authors.lastname: Sahu
authors.forename: Chai Lean
authors.initials: CL
authors.lastname: Teoh
authors.forename: Zhenzhen
authors.initials: Z
authors.lastname: Peng
authors.forename: Young-Tae
authors.initials: YT
authors.lastname: Chang
authors.forename: Alan J
authors.initials: AJ
authors.lastname: Davidson
mesh_terms: D000818:Animals; D005456:Fluorescent Dyes / Q000008:administration & dosage / Q000032:analysis*; D007668:Kidney / Q000033:anatomy & histology / Q000648:ultrastructure*; D007687:Kidney Tubules, Proximal / Q000033:anatomy & histology / Q000648:ultrastructure*; D007814:Larva / Q000033:anatomy & histology / Q000648:ultrastructure; D061848:Optical Imaging; D015027:Zebrafish / Q000033:anatomy & histology* / Q000254:growth & development
chemical_list: D005456:Fluorescent Dyes