title: Manipulation of chemical composition and architecture of non-biodegradable poly(ethylene terephthalate)/chitosan fibrous scaffolds and their effects on L929 cell behavior.
abstract: Microporous, non-woven fibrous scaffolds made of poly(ethylene terephthalate) and chitosan were produced by electrospinning. Fiber morphology, diameter, pore size, and wettability were manipulated by varying the chemical composition of the electrospinning solution, i.e. chitosan concentration and molecular weight, and by post-electrospinning treatment with glutaraldehyde. In vitro studies were conducted using a fibroblast cell line toward a comprehensive understanding of how scaffolds characteristics can modulate the cell behavior, i.e. viability, adhesion, proliferation, extracellular matrix secretion, and three-dimensional colonization. Substantial differences were found as a result of scaffold morphological changes. Higher levels of adhesion, spreading, and superficial proliferation were achieved for scaffolds with smaller fiber and pore diameters while cell penetration and internal colonization were enhanced for scaffolds with larger pores. Additionally, the available area for cell adhesion, which is related to fiber and pore size, was a crucial factor for the viability of L929 cells. This paper provides significant insights for the development and optimization of electrospun scaffolds toward an improved biological performance.
authors.affiliation: QOPNA Research Unit, Department of Chemistry, University of Aveiro, 3810-193 Aveiro, Portugal.
authors.forename: Beatriz
authors.initials: B
authors.lastname: Veleirinho
authors.affiliation: Integrated Technologies Laboratory, Chemical and Food Engineering Department, Federal University of Santa Catarina, 88040-900 Florianópolis, Brazil.
authors.forename: Fernanda V
authors.initials: FV
authors.lastname: Berti
authors.affiliation: Department of Cell Biology, Embryology and Genetics, Federal University of Santa Catarina, 88040-900 Florianópolis, Brazil.
authors.forename: Paulo F
authors.initials: PF
authors.lastname: Dias
authors.affiliation: Department of Plant Science, Federal University of Santa Catarina, 88040-900 Florianópolis, Brazil.
authors.forename: Marcelo
authors.initials: M
authors.lastname: Maraschin
authors.affiliation: Department of Pharmacology, Federal University of Santa Catarina, 88040-900 Florianópolis, Brazil.
authors.forename: Rosa M
authors.initials: RM
authors.lastname: Ribeiro-do-Valle
authors.affiliation: QOPNA Research Unit, Department of Chemistry, University of Aveiro, 3810-193 Aveiro, Portugal. Electronic address: jals@ua.pt.
authors.forename: José A
authors.initials: JA
authors.lastname: Lopes-da-Silva
mesh_terms: D000818:Animals; D017304:Annexin A5 / Q000378:metabolism; D001672:Biocompatible Materials / Q000737:chemistry / Q000494:pharmacology*; D002452:Cell Count; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D048271:Chitosan / Q000737:chemistry* / Q000494:pharmacology*; D003432:Cross-Linking Reagents / Q000737:chemistry; D003599:Cytoskeleton / Q000187:drug effects; D005347:Fibroblasts / Q000166:cytology* / Q000187:drug effects / Q000648:ultrastructure; D016650:Fluorescein-5-isothiocyanate / Q000378:metabolism; D051379:Mice; D008855:Microscopy, Electron, Scanning; D008970:Molecular Weight; D011093:Polyethylene Terephthalates / Q000737:chemistry* / Q000494:pharmacology*; D016062:Porosity; D013499:Surface Properties; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D017304:Annexin A5; D001672:Biocompatible Materials; D003432:Cross-Linking Reagents; D011093:Polyethylene Terephthalates; D048271:Chitosan; D016650:Fluorescein-5-isothiocyanate