title: Development and evaluation of a cucumber TILLING population.
abstract: BACKGROUND
  Ordered collections of mutants serve as invaluable tools in biological research. TILLING (Targeting Induced Local Lesions IN Genomes) provides an efficient method to discover, in mutagenized populations, the possible phenotypes controlled by gene sequences whose function is unknown. This method can replace transgenic techniques for the functional validation of cloned genes, especially in the case of transformation-recalcitrant plants such as cucumber.
  
  
  RESULTS
  We report the development of a TILLING cucumber population, generated by EMS mutagenesis in the Poinsett76 genetic background. The population was evaluated by screening for morphological mutations, and a range of developmental, pigmentation and spontaneous lesion mutants were observed. Suitability for detecting single nucleotide polymorphism in selected genes has been tested by screening a sample of amplicons, with detection rate of 1 SNP in ~1 Mbp.
  
  
  CONCLUSIONS
  The population described in this Research Note represents a useful asset in cucumber research, to be exploited for forward genetic screens and functional genomics purposes.
authors.forename: Rina
authors.initials: R
authors.lastname: Fraenkel
authors.forename: Irina
authors.initials: I
authors.lastname: Kovalski
authors.forename: Christelle
authors.initials: C
authors.lastname: Troadec
authors.forename: Abdelhafid
authors.initials: A
authors.lastname: Bendahmane
authors.affiliation: The Mina and Everard Goodman Faculty of Life Sciences, Bar-Ilan University, Ramat Gan, Israel. rafi.perl@gmail.com.
authors.forename: Rafael
authors.initials: R
authors.lastname: Perl-Treves
mesh_terms: D001483:Base Sequence; D001947:Breeding; D018553:Cucumis sativus / Q000033:anatomy & histology / Q000187:drug effects / Q000235:genetics*; D005020:Ethyl Methanesulfonate / Q000633:toxicity; D017343:Genes, Plant; D005820:Genetic Testing; D018745:Genome, Plant; D005838:Genotype; D008969:Molecular Sequence Data; D016296:Mutagenesis; D009153:Mutagens / Q000633:toxicity; D010641:Phenotype; D020641:Polymorphism, Single Nucleotide
chemical_list: D009153:Mutagens; D005020:Ethyl Methanesulfonate