title: [Expression of methionine sulfoxide reductase in colorectal cancer stem cells in vitro].
abstract: OBJECTIVE
  To investigate the expression of methionine sulfoxide reductase (MsrA) in colorectal cancer stem cells and its association with the tumorigenesis and progression of colorectal cancer.
  
  
  METHODS
  The CD133⁺/CD44⁺/ESA⁺ subpopulation of colorectal cancer cell line SW480 was obtained by magnetic activated cell sorting (MACS). The expression of MsrA, VEGF, MMP-13 and CXCR4 in the cancer cells, cancer stem cells and normal colon mucosa cells were detected using RT-PCR. The proliferation of colorectal cancer stem cells was evaluated with MTT assay.
  
  
  RESULTS
  The expression of MsrA was significantly higher in cancer stem cells than in the cancer cells and normal mucosa cells. Overexpression of MsrA inhibited the proliferation of colorectal cancer stem cells and down-regulated the expression of VEGF, MMP-13 and CXCR4.
  
  
  CONCLUSIONS
  MsrA suppresses the tumorigenesis and progression of colorectal cancer cells possibly by inhibiting cell proliferation and down-regulating VEGF, MMP-13 and CXCR4.
authors.affiliation: Department of Gastrointestinal Surgery, Fujian Provincial Hospital, Fujian Provincial Clinical College, Fujian Medical University, Fuzhou 350001,China. E-mail: xuefangqingsl@sina.com.
authors.forename: Fangqin
authors.initials: F
authors.lastname: Xue
authors.forename: Guohua
authors.initials: G
authors.lastname: Yang
authors.forename: Ruolei
authors.initials: R
authors.lastname: Huang
mesh_terms: D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015179:Colorectal Neoplasms / Q000201:enzymology; D015536:Down-Regulation; D006801:Humans; D053509:Matrix Metalloproteinase 13 / Q000378:metabolism; D051150:Methionine Sulfoxide Reductases / Q000378:metabolism*; D014411:Neoplastic Stem Cells / Q000201:enzymology*; D019718:Receptors, CXCR4 / Q000378:metabolism; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism
chemical_list: C527276:CXCR4 protein, human; D019718:Receptors, CXCR4; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A; D051150:Methionine Sulfoxide Reductases; C027219:methionine sulfoxide reductase; C507079:MMP13 protein, human; D053509:Matrix Metalloproteinase 13