title: Construction and characterization of an infectious cDNA clone of encephalomyocarditis virus from pigs in China.
abstract: Encephalomyocarditis virus (EMCV) infects animals of various species and causes a variety of clinical symptoms. In this study, an infectious full-length cDNA clone was constructed, and the characteristics of the rescued virus were investigated in vitro and in vivo. Our data demonstrated that the growth kinetics in vitro and plaque morphology of the rescued EMCV rNJ08 strain were similar to those of the parental strain. Although rNJ08 infected BALB/c mice, none of the mice died during the observation period of 14 days post-inoculation. The availability of the infectious cDNA clone provides a genetic platform for studying gene function and for the rational design of vaccines.
authors.affiliation: Key Laboratory of Animal Diseases Diagnostic and Immunology, Ministry of Agriculture, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, People's Republic of China.
authors.forename: Puxian
authors.initials: P
authors.lastname: Fang
authors.forename: Juan
authors.initials: J
authors.lastname: Bai
authors.forename: Xing
authors.initials: X
authors.lastname: Liu
authors.forename: Jing
authors.initials: J
authors.lastname: Dong
authors.forename: Tao
authors.initials: T
authors.lastname: Sun
authors.forename: Ping
authors.initials: P
authors.lastname: Jiang
mesh_terms: D000818:Animals; D018188:Cardiovirus Infections / Q000473:pathology / Q000821:virology*; D002681:China; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics* / Q000302:isolation & purification; D004195:Disease Models, Animal; D004686:Encephalomyocarditis virus / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification / Q000502:physiology*; D008807:Mice, Inbred BALB C; D016019:Survival Analysis; D013552:Swine / Q000821:virology*; D010948:Viral Plaque Assay
chemical_list: D018076:DNA, Complementary