title: Alterations in brain cerebral cortex proteome of rabies-infected cat.
abstract: Comparative proteome analysis using brain cerebral cortex tissues from cats and dogs infected with/without rabies virus were conducted using both two-dimensional gel-electrophoresis (2-DE) and 2-D fluorescence difference gel- electrophoresis (2D-DIGE) methods. The 2-DE gel images of all samples revealed >1,000 protein spots in each gel. Quantitative intensity analysis revealed the same overall protein pattern in certain regions of the gel, but the rabies-infected brains exhibited more protein spots than the non-infected controls. From approximately 880 protein spots detected by 2D-DIGE, 65 protein spots were increased and 46 were decreased. Eight of these protein spots were randomly selected and annotated by reference to previous known proteome data of rabid dog brains. They were similarly altered in both of the rabies-infected cats and dogs. A more detailed comparison of changes in proteomic profiles of brains between rabid cats and dogs should shed some light on the pathophysiological mechanism of rabies in domestic animals, as most rabies cases have been traceable to or believed to have originated from rabid dogs.
authors.forename: Songsri
authors.initials: S
authors.lastname: Kasempimolporn
authors.forename: Boonlert
authors.initials: B
authors.lastname: Lumlertdacha
authors.forename: Pannipa
authors.initials: P
authors.lastname: Chulasugandha
authors.forename: Supatsorn
authors.initials: S
authors.lastname: Boonchang
authors.forename: Visith
authors.initials: V
authors.lastname: Sitprija
mesh_terms: D000818:Animals; D001921:Brain / Q000473:pathology; D002371:Cat Diseases / Q000473:pathology*; D002415:Cats; D002540:Cerebral Cortex / Q000473:pathology*; D004283:Dog Diseases / Q000473:pathology*; D004285:Dogs; D015180:Electrophoresis, Gel, Two-Dimensional; D020543:Proteome; D011818:Rabies / Q000473:pathology*
chemical_list: D020543:Proteome