title: HPLC/QTOF-MS/MS application to investigate phenolic constituents from Ficus pandurata H. aerial roots.
abstract: Ficus pandurata H. aerial roots are used as a traditional Chinese medicine for the treatment of uarthritis, indigestion and hyperuricemia. However, the bioactive constituents responsible for the pharmacological effects of F. pandurata H. are unclear. A simple and efficient HPLC/QTOF-MS/MS (high-performance liquid chromatography/electrospray ionization with quadrupole time-of-flight tandem mass spectrometry) method was established to detect and identify active constituents in the n-butanol extract of F. pandurata H. aerial roots. Chemical constituents were separated and investigated by HPLC/QTOF-MS/MS in the negative-ion mode. Thirty-seven compounds, including hydroxycinnamic acid derivatives, hydroxybenzoic acid derivatives, hydroquinone glycosides, flavonoid glycosides, etc., were identified or tentatively characterized in the n-butanol extract of F. pandurata H. aerial roots by comparing the UV spectra, accurate mass spectra and fragmentation pathways and retrieving the reference literatures. Moreover, the flavonoid trisaccharides and hydroxybenzoic acid derivatives were tentatively characterized in F. pandurata H. for the first time. The analytical tool used here is very valuable in the rapid separation and identification of the multiple and minor constituents in the n-butanol extract of F. pandurata H. aerial roots.
authors.affiliation: College of Chemical Engineering, Zhejiang University of Technology, Hangzhou, 310014, China.|Key Laboratory of Organosilicon Chemistry and Material Technology, Hangzhou Normal University, Hangzhou, 310012, China.
authors.forename: Xiaoping
authors.initials: X
authors.lastname: Zhang
authors.affiliation: College of Pharmaceutical Sciences, Zhejiang Chinese Medical University, Hangzhou, Zhejiang, 310053, China.
authors.forename: Huiqing
authors.initials: H
authors.lastname: Lv
authors.affiliation: College of Chemical Engineering, Zhejiang University of Technology, Hangzhou, 310014, China.
authors.forename: Zuguang
authors.initials: Z
authors.lastname: Li
authors.affiliation: Key Laboratory of Organosilicon Chemistry and Material Technology, Hangzhou Normal University, Hangzhou, 310012, China.
authors.forename: Kezhi
authors.initials: K
authors.lastname: Jiang
authors.affiliation: Department of Chemistry, National Chung-Hsing University, Taichung, 40227, Taiwan.
authors.forename: Maw-Rong
authors.initials: MR
authors.lastname: Lee
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003373:Coumaric Acids / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D030681:Ficus / Q000737:chemistry*; D005419:Flavonoids / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D006027:Glycosides / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D010636:Phenols / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D010936:Plant Extracts / Q000737:chemistry; D018517:Plant Roots / Q000737:chemistry*; D053719:Tandem Mass Spectrometry / Q000379:methods
chemical_list: D003373:Coumaric Acids; D005419:Flavonoids; D006027:Glycosides; D010636:Phenols; D010936:Plant Extracts
keywords: F. pandurata H; HPLC/QTOF-MS/MS; aerial roots; fragmentation pathways; phenolic constituents