title: Docetaxel modulates the delayed rectifier potassium current (IK) and ATP-sensitive potassium current (IKATP) in human breast cancer cells.
abstract: Ion channel expression and activity may be affected during tumor development and cancer growth. Activation of potassium (K(+)) channels in human breast cancer cells is reported to be involved in cell cycle progression. In this study, we investigated the effects of docetaxel on the delayed rectifier potassium current (I K) and the ATP-sensitive potassium current (I KATP) in two human breast cancer cell lines, MCF-7 and MDA-MB-435S, using the whole-cell patch-clamp technique. Our results show that docetaxel inhibited the I K and I KATP in both cell lines in a dose-dependent manner. Compared with the control at a potential of +60 mV, treatment with docetaxel at doses of 0.1, 1, 5, and 10 µM significantly decreased the I K in MCF-7 cells by 16.1 ± 3.5, 30.2 ± 5.2, 42.5 ± 4.3, and 46.4 ± 9% (n = 5, P < 0.05), respectively and also decreased the I KATP at +50 mV. Similar results were observed in MDA-MB-435S cells. The G-V curves showed no significant changes after treatment of either MCF-7 or MDA-MB-435S cells with 10 μM docetaxel. The datas indicate that the possible mechanisms of I K and I KATP inhibition by docetaxel may be responsible for its effect on the proliferation of human breast cancer cells.
authors.affiliation: Department of Internal Oncology, Liaoning Cancer Hospital and Institute, Shenyang, 110042, China, jianong@126.com.
authors.forename: Tao
authors.initials: T
authors.lastname: Sun
authors.forename: Zhi-Guo
authors.initials: ZG
authors.lastname: Song
authors.forename: Da-Qing
authors.initials: DQ
authors.lastname: Jiang
authors.forename: Hong-Guang
authors.initials: HG
authors.lastname: Nie
authors.forename: Dong-Yun
authors.initials: DY
authors.lastname: Han
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D001943:Breast Neoplasms / Q000378:metabolism*; D045744:Cell Line, Tumor; D051668:Delayed Rectifier Potassium Channels / Q000378:metabolism*; D000077143:Docetaxel; D005260:Female; D006801:Humans; D054086:KATP Channels / Q000378:metabolism*; D061986:MCF-7 Cells; D008564:Membrane Potentials / Q000187:drug effects*; D018408:Patch-Clamp Techniques; D043823:Taxoids / Q000494:pharmacology*
chemical_list: D000970:Antineoplastic Agents; D051668:Delayed Rectifier Potassium Channels; D054086:KATP Channels; D043823:Taxoids; D000077143:Docetaxel