title: Langmuir monolayers and thin films of amphifilic thiacalix[4]arenes. Properties and matrix for the immobilization of cytochrome c.
abstract: Formation and properties of Langmuir films of thiacalix[4]arene (TCA) derivatives containing N-donor groups on the lower rim (Y═O(CH2)3CN; OCH2CN; NH2; OCH2ArCN-p) in 1,3-alternate conformation on aqueous subphase and solid substrates have been studied. Only tetra-cyanopropoxy-p-tert-butylthiacalix[4]arene 1 forms a typical monomolecular layer with perpendicular orientation of the macrocycle relative to the water-air interface that is able to immobilize cytochrome c in the entire range of the surface pressure. Obtained monolayers were transferred by Langmuir-Schaefer technique onto quartz, indium-tin oxide (ITO), and silicon. It was demonstrated that protein activity is retained after immobilization on the substrate.
authors.affiliation: A.E. Arbuzov Institute of Organic and Physical Chemistry, Russian Academy of Sciences , Arbuzov st. 8, Kazan 420088, Russia.
authors.forename: Svetlana E
authors.initials: SE
authors.lastname: Solovieva
authors.forename: Roman A
authors.initials: RA
authors.lastname: Safiullin
authors.forename: Evgeni N
authors.initials: EN
authors.lastname: Kochetkov
authors.forename: Nina B
authors.initials: NB
authors.lastname: Melnikova
authors.forename: Marsil K
authors.initials: MK
authors.lastname: Kadirov
authors.forename: Elena V
authors.initials: EV
authors.lastname: Popova
authors.forename: Igor S
authors.initials: IS
authors.lastname: Antipin
authors.forename: Alexander I
authors.initials: AI
authors.lastname: Konovalov
mesh_terms: D000818:Animals; D002417:Cattle; D045304:Cytochromes c / Q000737:chemistry*; D004800:Enzymes, Immobilized / Q000737:chemistry*; D057927:Hydrophobic and Hydrophilic Interactions; D008958:Models, Molecular; D008968:Molecular Conformation; D010636:Phenols / Q000737:chemistry*; D013440:Sulfides / Q000737:chemistry*; D013499:Surface Properties; D014867:Water / Q000737:chemistry
chemical_list: D004800:Enzymes, Immobilized; D010636:Phenols; D013440:Sulfides; C500450:thiacalix(4)arene; D014867:Water; D045304:Cytochromes c