title: Protection by the flavonoids quercetin and luteolin against peroxide- or menadione-induced oxidative stress in MC3T3-E1 osteoblast cells.
abstract: Potential protective effects of the flavonoids quercetin and luteolin have been examined against the oxidative stress of MC3T3-E1 osteoblast-like cells. Although hydrogen peroxide and menadione reduced cell viability, the toxicity was prevented by desferrioxamine or catalase but not superoxide dismutase, suggesting the involvement of hydrogen peroxide in both cases. Quercetin and luteolin reduced the oxidative damage, especially that caused by hydrogen peroxide. When cultures were pre-incubated with quercetin or luteolin, protection was reduced or lost. Protection was also reduced when a 24 h pre-incubation with the flavonoids was followed by exposure to menadione alone. Pretreating cultures with luteolin impaired protection by quercetin, whereas quercetin pretreatment did not affect protection by luteolin. It is concluded that quercetin and luteolin suppress oxidative damage to MC3T3-E1 cells, especially caused by peroxide. The reduction in protection by pretreatment implies a down-regulation of part of the toxic transduction pathway.
authors.affiliation: a College of Medical, Veterinary & Life Sciences, Institute of Neuroscience and Psychology, University of Glasgow , Glasgow G12 8QQ , UK.
authors.forename: Amos A
authors.initials: AA
authors.lastname: Fatokun
authors.forename: Mercedes
authors.initials: M
authors.lastname: Tome
authors.forename: Robert A
authors.initials: RA
authors.lastname: Smith
authors.forename: L Gail
authors.initials: LG
authors.lastname: Darlington
authors.forename: Trevor W
authors.initials: TW
authors.lastname: Stone
mesh_terms: D016475:3T3 Cells; D000818:Animals; D006861:Hydrogen Peroxide / Q000009:adverse effects; D047311:Luteolin / Q000494:pharmacology*; D051379:Mice; D010006:Osteoblasts / Q000187:drug effects*; D018384:Oxidative Stress / Q000187:drug effects*; D011794:Quercetin / Q000494:pharmacology*; D024483:Vitamin K 3 / Q000009:adverse effects
chemical_list: D024483:Vitamin K 3; D011794:Quercetin; D006861:Hydrogen Peroxide; D047311:Luteolin
keywords: bone; flavonoids; luteolin; osteoblasts; oxidative stress; quercetin