title: RP-HPLC-DAD method for the identification of two potential antioxidant agents namely verminoside and 1-O-(E)-caffeoyl-β-gentiobiose from Spathodea campanulata leaves.
abstract: A simple and reliable high-performance liquid chromatographic method was successfully developed for the study of fingerprint chromatograms of extract and fractions from the leaves of Spathodea campanulata (SC) using verminoside (1) and 1-O-(E)-caffeoyl-β-gentiobiose (2) as marker compounds. Antioxidant activity of SC was determined by using free radical of 2,2-diphenyl-1-picryl-hydrazyl-hydrate as an experimental model. The docking study of selected target, tyrosinase and ligands (ascorbic acid, compounds 1 and 2) was performed through Autodock Vina v0.8. Fingerprints of methanol, chloroform, ethylacetate, n-butanol and water extracts could resolve 13, 11, 22, 16 and 5 peaks, respectively. Extract, fractions and compounds 1 and 2 previously isolated from SC displayed remarkable antioxidant activity with radical-scavenging activity ranging from 2.5 to 6.7 μg/mL. In silico study identified compounds 1 and 2 as potential inhibitors of tyrosinase correlating with the observed antioxidant activity in vitro.
authors.affiliation: a CSIR-TWAS Fellow, Department of Biochemistry, Faculty of Science , University of Dschang , P.O. Box-67, Dschang , Cameroon.
authors.forename: Pone Kamdem
authors.initials: PK
authors.lastname: Boniface
authors.forename: Manju
authors.initials: M
authors.lastname: Singh
authors.forename: Surjeet
authors.initials: S
authors.lastname: Verma
authors.forename: Aparna
authors.initials: A
authors.lastname: Shukla
authors.forename: Feroz
authors.initials: F
authors.lastname: Khan
authors.forename: Santosh Kumar
authors.initials: SK
authors.lastname: Srivastava
authors.forename: Anirban
authors.initials: A
authors.lastname: Pal
mesh_terms: D000975:Antioxidants / Q000737:chemistry* / Q000302:isolation & purification; D029402:Bignoniaceae / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004187:Disaccharides / Q000737:chemistry* / Q000302:isolation & purification; D039823:Iridoids / Q000737:chemistry* / Q000302:isolation & purification; D015394:Molecular Structure; D010936:Plant Extracts / Q000737:chemistry; D018515:Plant Leaves / Q000737:chemistry
chemical_list: C000595470:1-O-(E)-caffeoyl-beta-gentiobiose; D000975:Antioxidants; D004187:Disaccharides; D039823:Iridoids; D010936:Plant Extracts; C506490:verminoside
keywords: HPLC; Spathodea campanulata; antioxidant; chromatogram; docking; fingerprint