title: Hematopoietic stem cell capture and directional differentiation into vascular endothelial cells for metal stent-coated chitosan/hyaluronic acid loading CD133 antibody.
abstract: A series of metal stents coated with chitosan/hyaluronic acid (CS/HA) loading antibodies by electrostatic self-assembled method were prepared, and the types of cells captured by antibodies and their differentiation in vascular endothelial cells (ECs) evaluated by molecular biology and scanning electron microscope. The results showed that CD133 stent can selectively capture hematopoietic stem cells (HSC),which directionally differentiate into vascular ECs in peripheral blood by (CS/HA) induction, and simultaneously inhibit migration and proliferation of immune cells and vascular smooth muscle cells (MCs). CD34 stent can capture HSC, hematopoietic progenitor cells that differentiate into vascular ECs and immune cells, promoting smooth MCs growth, leading to thrombosis, inflammation, and rejection. CD133 stent can be implanted into miniature pig heart coronary and can repair vascular damage by capturing own HSC, thus contributing to the rapid natural vascular repair, avoiding inflammation and rejection, thrombosis and restenosis. These studies demonstrated that CD133 stent of HSC capture will be an ideal coated metal stent providing a new therapeutic approach for cardiovascular and cerebrovascular disease.
authors.affiliation: 1 State Key Laboratory of Fine Chemicals, School of Pharmaceutical Science and Technology, Dalian University of Technology , Dalian, China .
authors.forename: Shixuan
authors.initials: S
authors.lastname: Zhang
authors.forename: Fan
authors.initials: F
authors.lastname: Zhang
authors.forename: Bo
authors.initials: B
authors.lastname: Feng
authors.forename: Qingyu
authors.initials: Q
authors.lastname: Fan
authors.forename: Feng
authors.initials: F
authors.lastname: Yang
authors.forename: Debin
authors.initials: D
authors.lastname: Shang
authors.forename: Jinghan
authors.initials: J
authors.lastname: Sui
authors.forename: Hong
authors.initials: H
authors.lastname: Zhao
mesh_terms: D000071916:AC133 Antigen; D000818:Animals; D000906:Antibodies / Q000494:pharmacology*; D015703:Antigens, CD / Q000276:immunology*; D002454:Cell Differentiation / Q000187:drug effects*; D048271:Chitosan / Q000494:pharmacology*; D020099:Coated Materials, Biocompatible / Q000494:pharmacology; D042783:Endothelial Cells / Q000166:cytology* / Q000187:drug effects; D006023:Glycoproteins / Q000276:immunology*; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000187:drug effects; D006801:Humans; D006820:Hyaluronic Acid / Q000494:pharmacology*; D008670:Metals / Q000494:pharmacology; D010455:Peptides / Q000276:immunology*; D015607:Stents; D034421:Sus scrofa
chemical_list: D000071916:AC133 Antigen; D000906:Antibodies; D015703:Antigens, CD; D020099:Coated Materials, Biocompatible; D006023:Glycoproteins; D008670:Metals; C000606023:PROM1 protein, human; D010455:Peptides; D006820:Hyaluronic Acid; D048271:Chitosan