title: Evaluation of multiple antigenic peptides based on the Chikungunya E2 protein for improved serological diagnosis of infection.
abstract: In recent years, Chikungunya virus (CHIKV) reemerged and numerous outbreaks were reported all over the world. After screening CHIKV-positive sera, we had already reported many dominant epitopes within the envelope E2 protein of CHIKV. In the present study, we aimed at developing a highly sensitive immunodiagnostic assay for CHIKV based on a multiple antigenic peptide (MAP) approach using selective epitopes of the E2 protein. MAPs in four different E2 peptide combinations were screened with CHIKV-positive sera. The MAPs reacted with all CHIKV-positive sera and no reactivity was seen with healthy or dengue-positive sera. Our results indicate that MAP 1 seems to be an alternate antigen to full-length protein E2 for immunodiagnosis of CHIKV infections with high sensitivity and specificity.
authors.affiliation: 1 Department of Biochemistry, All India Institute of Medical Sciences , New Delhi, India .
authors.forename: Santwana
authors.initials: S
authors.lastname: Bhatnagar
authors.forename: Pradeep
authors.initials: P
authors.lastname: Kumar
authors.forename: Teena
authors.initials: T
authors.lastname: Mohan
authors.forename: Priyanka
authors.initials: P
authors.lastname: Verma
authors.forename: M M
authors.initials: MM
authors.lastname: Parida
authors.forename: S L
authors.initials: SL
authors.lastname: Hoti
authors.forename: D N
authors.initials: DN
authors.lastname: Rao
mesh_terms: D000914:Antibodies, Viral / Q000097:blood*; D065632:Chikungunya Fever / Q000175:diagnosis*; D002646:Chikungunya virus / Q000276:immunology*; D006801:Humans; D011994:Recombinant Proteins; D012680:Sensitivity and Specificity; D012698:Serologic Tests / Q000379:methods; D014759:Viral Envelope Proteins
chemical_list: D000914:Antibodies, Viral; D011994:Recombinant Proteins; D014759:Viral Envelope Proteins