title: Brassica rapa.
abstract: Within this chapter we outline an A. tumefaciens-mediated transformation method for B. rapa using 4-day-old cotyledonary explants and the genotype R-o-18. Transformation efficiencies are typically achieved in the region of 1% (based on 2 PCR-positive independent shoots from 200 inoculated explants). This system has been developed to work with gentamicin selection.
authors.affiliation: Department of Crop Genetics, John Innes Centre, Norwich Research Park, Norwich, NR4 7UH, UK.
authors.forename: Tom
authors.initials: T
authors.lastname: Lawrenson
authors.forename: Cassandra
authors.initials: C
authors.lastname: Goldsack
authors.forename: Lars
authors.initials: L
authors.lastname: Ostergaard
authors.forename: Penny A C Hundleby
authors.initials: PA
authors.lastname: née Sparrow
mesh_terms: D016960:Agrobacterium tumefaciens / Q000235:genetics / Q000254:growth & development; D001937:Brassica / Q000235:genetics* / Q000254:growth & development*; D018920:Coculture Techniques; D018548:Cotyledon / Q000235:genetics / Q000254:growth & development; D004780:Environment, Controlled; D005818:Genetic Engineering / Q000379:methods*; D018525:Germination; D018520:Plant Shoots / Q000235:genetics / Q000254:growth & development; D013242:Sterilization; D014170:Transformation, Genetic