title: Effect of dietary astaxanthin on free radical scavenging capacity and nitrite stress tolerance of postlarvae shrimp, Pleoticus muelleri.
abstract: The aim of this study was to investigate the effect of astaxanthin feed supplementation and environmental nitrite stress in postlarvae of Pleoticus muelleri (15 ± 0.004 mg initial weight) under culture conditions. Diets containing three levels of astaxanthin, 0 mg kg(-1) of diet (C0), 100 mg kg(-1) of diet (C(100)), and 300 mg kg(-1) of diet (C(300)), were used. Postlarvae fed with each diet were exposed to different concentrations of nitrite (NO(2)Na) (0-200 mg L(-1)). The 96 h median lethal concentration (LC50) values of nitrite N were 76.3, 89.7, and 157 mg L(-1) for shrimps fed to C0, C(100), and C(300). The scavenging properties were evaluated against the stable 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical by electron resonance spectroscopy (EPR). For all feed treatments, the extracts exhibited strong DPPH scavenging activity; however, shrimp fed with C(100) and C(300) showed the greatest activity to quench DPPH (62 and 59%, respectively) with respect to C0 (43%). It can be concluded that astaxanthin acts as a protector of nitrite stress in P. muelleri.
authors.affiliation: Instituto de Investigaciones Marinas y Costeras, Universidad Nacional de Mar del Plata (UNMDP)-Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET) , Funes 3350, 7600 Mar del Plata, Argentina.
authors.forename: Ana Cristina
authors.initials: AC
authors.lastname: Díaz
authors.forename: Susana María
authors.initials: SM
authors.lastname: Velurtas
authors.forename: María Laura
authors.initials: ML
authors.lastname: Espino
authors.forename: Jorge Lino
authors.initials: JL
authors.lastname: Fenucci
mesh_terms: D000821:Animal Feed / Q000032:analysis*; D000818:Animals; D033364:Decapoda / Q000737:chemistry / Q000254:growth & development / Q000502:physiology*; D019587:Dietary Supplements / Q000032:analysis*; D016166:Free Radical Scavengers / Q000032:analysis / Q000378:metabolism*; D005609:Free Radicals / Q000378:metabolism; D007814:Larva / Q000254:growth & development / Q000378:metabolism; D009573:Nitrites / Q000378:metabolism*; D013312:Stress, Physiological; D024341:Xanthophylls / Q000032:analysis / Q000378:metabolism
chemical_list: D016166:Free Radical Scavengers; D005609:Free Radicals; D009573:Nitrites; D024341:Xanthophylls; C005948:astaxanthine
keywords: Pleoticus muelleri; astaxanthin; histopathology; nitrite; scavenging capacity