title: MicroRNA-204-5p inhibits gastric cancer cell proliferation by downregulating USP47 and RAB22A.
abstract: MicroRNAs (miRNAs) are a type of small noncoding RNAs that are strongly implicated in carcinogenesis. However, the potential diagnostic, prognostic and therapeutic roles of the majority of miRNAs in the pathological processes of tumorigenesis remain largely unknown. Our and others' data revealed that miR-204-5p was significantly downregulated in gastrointestinal tumor tissues compared with adjacent noncancerous tissues. The downregulation of miR-204-5p was confirmed in our gastric cancer (GC) cohort, and we showed that ectopic expression of miR-204-5p inhibited, whereas silencing miR-204-5p expression promoted GC cell proliferation in vitro. Subsequent mechanistic investigations identified that USP47 and RAB22A are direct functional targets of miR-204-5p in GC. Silencing the expression of USP47 and RAB22A using siRNA phenocopied the proliferation-inhibiting function of miR-204-5p in GC cells. Our results uncovered that miR-204-5p acts as a tumor suppressor in GC through inhibiting USP47 and RAB22A.
authors.affiliation: Oncology Institute, The Fourth Affiliated Hospital of Soochow University, The Fourth People's Hospital of Wuxi, Wuxi, 214062, Jiangsu Province, China.
authors.forename: Binbin
authors.initials: B
authors.lastname: Zhang
authors.forename: Yuan
authors.initials: Y
authors.lastname: Yin
authors.forename: Yaling
authors.initials: Y
authors.lastname: Hu
authors.forename: Jiwei
authors.initials: J
authors.lastname: Zhang
authors.forename: Zehua
authors.initials: Z
authors.lastname: Bian
authors.forename: Mingxu
authors.initials: M
authors.lastname: Song
authors.forename: Dong
authors.initials: D
authors.lastname: Hua
authors.forename: Zhaohui
authors.initials: Z
authors.lastname: Huang
mesh_terms: D015153:Blotting, Western; D049109:Cell Proliferation; D015536:Down-Regulation; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics*; D006801:Humans; D007150:Immunohistochemistry; D035683:MicroRNAs / Q000235:genetics*; D034741:RNA, Small Interfering; D060888:Real-Time Polymerase Chain Reaction; D013274:Stomach Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D043222:Ubiquitin Thiolesterase / Q000096:biosynthesis* / Q000235:genetics; D064570:Ubiquitin-Specific Proteases; D020691:rab GTP-Binding Proteins / Q000096:biosynthesis* / Q000235:genetics
chemical_list: C549404:MIRN204 microRNA, human; D035683:MicroRNAs; C484191:RAB22A protein, human; D034741:RNA, Small Interfering; C557800:USP47 protein, human; D043222:Ubiquitin Thiolesterase; D064570:Ubiquitin-Specific Proteases; D020691:rab GTP-Binding Proteins