title: Characterization of exoelectrogenic bacteria enterobacter strains isolated from a microbial fuel cell exposed to copper shock load.
abstract: Microorganisms capable of generating electricity in microbial fuel cells (MFCs) have gained increasing interest. Here fourteen exoelectrogenic bacterial strains were isolated from the anodic biofilm in an MFC before and after copper (Cu) shock load by Hungate roll-tube technique with solid ferric (III) oxide as an electron acceptor and acetate as an electron donor. Phylogenetic analysis of the 16S rRNA gene sequences revealed that they were all closely related to Enterobacter ludwigii DSM 16688T within the Enterobacteriaceae family, although these isolated bacteria showed slightly different morphology before and after Cu shock load. Two representative strains R2B1 (before Cu shock load) and B4B2 (after Cu shock load) were chosen for further analysis. B4B2 is resistant to 200 mg L-1 of Cu(II) while R2B1 is not, which indicated the potential selection of the Cu shock load. Raman analysis revealed that both R2B1 and B4B2 contained c-type cytochromes. Cyclic voltammetry measurements revealed that strain R2B1 had the capacity to transfer electrons to electrodes. The experimental results demonstrated that strain R2B1 was capable of utilizing a wide range of substrates, including Luria-Bertani (LB) broth, cellulose, acetate, citrate, glucose, sucrose, glycerol and lactose to generate electricity, with the highest current density of 440 mA·m-2 generated from LB-fed MFC. Further experiments indicated that the bacterial cell density had potential correlation with the current density.
authors.affiliation: Institute of Urban Environment, Chinese Academy of Sciences, Xiamen, 361021, China; Ningbo Urban Environment Observation and Research Station-NUEORS, Chinese Academy of Sciences, Ningbo, 351800, China.
authors.forename: Cuijie
authors.initials: C
authors.lastname: Feng
authors.affiliation: Institute of Urban Environment, Chinese Academy of Sciences, Xiamen, 361021, China; Ningbo Urban Environment Observation and Research Station-NUEORS, Chinese Academy of Sciences, Ningbo, 351800, China.
authors.forename: Jiangwei
authors.initials: J
authors.lastname: Li
authors.affiliation: Institute of Urban Environment, Chinese Academy of Sciences, Xiamen, 361021, China; Ningbo Urban Environment Observation and Research Station-NUEORS, Chinese Academy of Sciences, Ningbo, 351800, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Qin
authors.affiliation: Institute of Urban Environment, Chinese Academy of Sciences, Xiamen, 361021, China.
authors.forename: Lixiang
authors.initials: L
authors.lastname: Chen
authors.affiliation: Institute of Urban Environment, Chinese Academy of Sciences, Xiamen, 361021, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Zhao
authors.affiliation: Institute of Urban Environment, Chinese Academy of Sciences, Xiamen, 361021, China; Ningbo Urban Environment Observation and Research Station-NUEORS, Chinese Academy of Sciences, Ningbo, 351800, China.
authors.forename: Shaohua
authors.initials: S
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Microbial Metabolism, Shanghai Jiao Tong University, Shanghai, 200240, China.
authors.forename: Hongbo
authors.initials: H
authors.lastname: Hu
authors.affiliation: Institute of Urban Environment, Chinese Academy of Sciences, Xiamen, 361021, China; Ningbo Urban Environment Observation and Research Station-NUEORS, Chinese Academy of Sciences, Ningbo, 351800, China.
authors.forename: Chang-Ping
authors.initials: CP
authors.lastname: Yu
mesh_terms: D001674:Bioelectric Energy Sources / Q000382:microbiology*; D003300:Copper / Q000502:physiology*; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D004560:Electricity; D004579:Electron Transport; D004754:Enterobacter / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000032:analysis; D017422:Sequence Analysis, DNA; D013059:Spectrum Analysis, Raman
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D003300:Copper