title: [Experimental study on effect of burn on brown adipose tissue in mice].
abstract: OBJECTIVE
  To investigate the effect of burn on brown adipose tissue (BAT) in BALB/c mice.
  
  
  METHODS
  Forty 3-4 months old male BALB/c mice with initial body weight of (20 ± 3) g were randomly divided into control group and burn group (n = 20). BALB/c mice in burn group were subjected to a 30% total body surface area (TBSA) full-thickness thermal injury. BALB/c mice in control group were not treated. The body weight and temperature were observed before and after burn. At 7 days after burn, morphological changes of white adipose tissue (WAT) and BAT were observed, the gene and protein expressions of uncoupling protein 1 (UCP-1) were detected.
  
  
  RESULTS
  There was no significant difference in the body weight and body temperature before burn (P > 0.05). At 1, 2, 3, and 4 weeks after burn, the body weight was significantly lower in burn group than in control group (P < 0.05). At 1, 2, 3, and 7 days after burn, the body temperature was significantly higher in burn group than in control group (P < 0.05). At 7 days after burn, the weight of WAT was significantly reduced, and the weight of BAT was significantly increased in burn group (P < 0.05); WAT and BAT cells became smaller, cell number increased, the cytoplasm and mitochondria appeared as compact. The UCP-1 gene and protein expressions of burn group were significantly higher than those of control group (P < 0.05).
  
  
  CONCLUSIONS
  BAT plays an important role in burn-induced hypermetabolism.
authors.forename: Shubo
authors.initials: S
authors.lastname: Zhuang
authors.forename: Jiake
authors.initials: J
authors.lastname: Chai
authors.forename: Yonghui
authors.initials: Y
authors.lastname: Yu
authors.forename: Huinan
authors.initials: H
authors.lastname: Yin
authors.forename: Lingying
authors.initials: L
authors.lastname: Liu
authors.forename: Jun
authors.initials: J
authors.lastname: Fan
authors.forename: Yihe
authors.initials: Y
authors.lastname: Wang
authors.forename: Yusen
authors.initials: Y
authors.lastname: Hou
mesh_terms: D002001:Adipose Tissue, Brown / Q000502:physiology*; D052436:Adipose Tissue, White / Q000502:physiology; D000818:Animals; D001831:Body Temperature; D001835:Body Weight; D002056:Burns / Q000150:complications*; D007473:Ion Channels / Q000235:genetics* / Q000378:metabolism*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D024101:Mitochondrial Proteins / Q000235:genetics* / Q000378:metabolism*; D013696:Temperature; D000071256:Uncoupling Protein 1
chemical_list: D007473:Ion Channels; D024101:Mitochondrial Proteins; C000604372:Ucp1 protein, mouse; D000071256:Uncoupling Protein 1