title: Pyrosequencing of the CYP2C9 -1766T>C polymorphism as a means of detecting the CYP2C9*8 allele.
abstract: The CYP2C9 c.449G>A (p.R150H, rs7900194) polymorphism, which confers the CYP2C9*8 allele, is common in persons of African descent and results in reduced clearance of the narrow therapeutic index drugs, warfarin and phenytoin. Because of significant homology in DNA sequence at the 449G>A locus among CYP2C genes, the 449G>A variant cannot be reliably detected via PCR-based genotyping assays that require a short PCR product, such as pyrosequencing. Herein, we propose genotyping for the CYP2C9 c.-1766T>C polymorphism via pyrosequencing as an alternative and accurate means of identifying the CYP2C9*8 allele.
authors.affiliation: Department of Pharmacy Practice, University of Illinois at Chicago, Chicago, IL, USA.
authors.forename: Shitalben R
authors.initials: SR
authors.lastname: Patel
authors.forename: Taimour Y
authors.initials: TY
authors.lastname: Langaee
authors.forename: Shan S
authors.initials: SS
authors.lastname: Wong
authors.forename: Larisa H
authors.initials: LH
authors.lastname: Cavallari
mesh_terms: D000483:Alleles; D065729:Cytochrome P-450 CYP2C9 / Q000235:genetics*; D005838:Genotype; D006801:Humans; D020641:Polymorphism, Single Nucleotide; D017422:Sequence Analysis, DNA
chemical_list: D065729:Cytochrome P-450 CYP2C9
keywords: CYP2C9 -1766T>C; CYP2C9*8; genotyping; pyrosequencing; warfarin