title: Mobile app-based quantitative scanometric analysis.
abstract: The feasibility of using smartphones and other mobile devices as the detection platform for quantitative scanometric assays is demonstrated. The different scanning modes (color, grayscale, black/white) and grayscale converting protocols (average, weighted average/luminosity, and software specific) have been compared in determining the optical darkness ratio (ODR) values, a conventional quantitation measure for scanometric assays. A mobile app was developed to image and analyze scanometric assays, as demonstrated by paper-printed tests and a biotin-streptavidin assay on a plastic substrate. Primarily for ODR analysis, the app has been shown to perform as well as a traditional desktop scanner, augmenting that smartphones (and other mobile devices) promise to be a practical platform for accurate, quantitative chemical analysis and medical diagnostics.
authors.affiliation: Department of Chemistry and ‡Department of Molecular Biology and Biochemistry, Simon Fraser University , Burnaby, British Columbia V5A 1S6, Canada.
authors.forename: Jessica X H
authors.initials: JX
authors.lastname: Wong
authors.forename: Frank S F
authors.initials: FS
authors.lastname: Liu
authors.forename: Hua-Zhong
authors.initials: HZ
authors.lastname: Yu
mesh_terms: D040421:Cell Phone; D002623:Chemistry Techniques, Analytical / Q000295:instrumentation* / Q000379:methods*; D003720:Densitometry; D063731:Mobile Applications