title: Nanoglassified, optically-active monolayer films of gold nanoparticles for in situ orthogonal detection by localized surface plasmon resonance and surface-assisted laser desorption/ionization-MS.
abstract: Localized surface plasmon resonance (LSPR) represents a sensitive and versatile method for detection of biomolecules in a label-free fashion, but identification of bound analytes can be challenging with LSPR alone, especially for samples in a complex medium. We report the fabrication of an optically active, plasmonic film of gold nanoparticles by using a self-assembly and calcination process, which offers orthogonal measurements enabling multifaceted characterization on the same surface with LSPR and surface-assisted laser desorption/ionization mass spectrometry. This proof-of-concept study involves plasmonic characterization of the fabricated nanofilm, real-time monitoring of vesicle-surface interactions toward formation of fluid lipid bilayer, and mass spectrometric analysis of peptides and cytochrome c digest. This multifunction-enabling surface material can yield complementary analytical information, providing new tools for comprehensive analysis of biomolecular samples.
authors.affiliation: Department of Chemistry, University of California , Riverside, California 92521, United States.
authors.forename: Chih-Yuan
authors.initials: CY
authors.lastname: Chen
authors.forename: Samuel S
authors.initials: SS
authors.lastname: Hinman
authors.forename: Jicheng
authors.initials: J
authors.lastname: Duan
authors.forename: Quan
authors.initials: Q
authors.lastname: Cheng
mesh_terms: D002623:Chemistry Techniques, Analytical / Q000295:instrumentation*; D006046:Gold / Q000737:chemistry*; D007834:Lasers; D013058:Mass Spectrometry; D053768:Metal Nanoparticles / Q000737:chemistry*; D020349:Surface Plasmon Resonance; D013499:Surface Properties
chemical_list: D006046:Gold