title: Enzyme linked immunosorbent assay for quantification of bovine interleukin-8 to study infection and immunity in the female genital tract.
abstract: OBJECTIVE
  The chemokine IL-8 recruits neutrophils to sites of infection, including the endometrium of the bovine uterus. However, quantification of bovine IL-8 often yields lower concentrations than for other species, which may reflect impaired innate immune responses by bovine cells or inaccurate measurement of IL-8 using the current human IL-8 ELISA method.
  
  
  METHODS
  An ELISA was developed and validated for detection of bovine IL-8. Utility of the assay was tested by measuring the response of bovine endometrium and cells to bacteria and pathogen-associated molecular patterns.
  
  
  RESULTS
  The developed ELISA detected 62.5-2000 pg/mL IL-8, with minimal cross-reactivity to other inflammatory mediators. Concentrations of bovine IL-8 were measured more accurately by the bovine than human IL-8 ELISA. Bovine endometrial IL-8 responses to pathogen-associated molecules were quantitatively similar to other species.
  
  
  CONCLUSIONS
  A bovine-specific IL-8 ELISA was developed, which accurately measured IL-8 secretion from endometrial cells.
authors.affiliation: Institute of Life Science, College of Medicine, Swansea University, Swansea, UK.
authors.forename: James G
authors.initials: JG
authors.lastname: Cronin
authors.forename: Rebecca
authors.initials: R
authors.lastname: Hodges
authors.forename: Sara
authors.initials: S
authors.lastname: Pedersen
authors.forename: Iain M
authors.initials: IM
authors.lastname: Sheldon
mesh_terms: D000818:Animals; D002417:Cattle; D004717:Endometrium / Q000276:immunology / Q000382:microbiology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D005836:Genitalia, Female / Q000276:immunology* / Q000382:microbiology*; D016209:Interleukin-8 / Q000276:immunology*; D060737:Reproductive Tract Infections / Q000276:immunology*
chemical_list: D016209:Interleukin-8
keywords: Bovine; ELISA; endometrium; inflammation; interleukin-8