title: Water-extracted Ampelopsis brevipedunculata downregulates IL-1β,CCL5, and COX-2 expression via inhibition of PKC-mediated JNK/NF-κB signaling pathways in human monocytic cells.
abstract: The anti-inflammatory and anti-hepatotoxic effects of Ampelopsis brevipedunculata (A.bre) have been well known in folk medicine. An ethanol-extract of A.bre has been reported to inhibit carbon tetrachloric acid induced hepatic injury, suggesting that extracted components from A.bre could potentially treat inflammatory disease. To test this hypothesis, in this study, we extracted polysaccharide components from leaves of A.bre and investigated the anti-inflammatory effects in PMA stimulated THP-1 cells. THP-1 cells activated by PMA in the presence or absence of A.bre demonstrated that a water-extract of A.bre inhibited the expression of pro-inflammatory cytokine IL-1β and chemokine CCL-5 in a dose-dependent manner. In addition, A.bre suppressed production of cyclooxygenase (COX)-2 in THP-1 cells activated by PMA. Moreover, A.bre markedly down-regulated the expression of p-JNK1/3, whereas it did not inhibit production of the phosphorylated form of p38 and extracellular signal-regulated kinase in THP-1 cells treated by PMA. Particularly, A.bre inhibited the translocation of transcription factor NF-κB from the cytosol into the nucleus in PMA-stimulated THP-1 cells. Collectively, our data showed that water-extracted A.bre inhibited the protein kinase C-JNKs/NF-κB signaling pathways, resulting in the suppression of IL-1β, CCL-5, and COX-2 expression. This study suggests that water extracted A.bre may be a therapeutic agent against inflammatory disease.
authors.affiliation: Laboratory of Cellular Biology & Immunobiochemistry, Department of Bioscience & Biotechnology, Konkuk University, Korea.
authors.forename: Minh Quan
authors.initials: MQ
authors.lastname: Le
authors.forename: Man Sub
authors.initials: MS
authors.lastname: Kim
authors.forename: Yong Seok
authors.initials: YS
authors.lastname: Song
authors.forename: Whoe Nyoung
authors.initials: WN
authors.lastname: Noh
authors.forename: Se Chul
authors.initials: SC
authors.lastname: Chun
authors.forename: Do Young
authors.initials: DY
authors.lastname: Yoon
mesh_terms: D032421:Ampelopsis / Q000737:chemistry*; D000893:Anti-Inflammatory Agents; D002478:Cells, Cultured; D018946:Chemokine CCL5 / Q000378:metabolism*; D051546:Cyclooxygenase 2 / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D015536:Down-Regulation / Q000187:drug effects*; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D007249:Inflammation / Q000188:drug therapy; D018836:Inflammation Mediators / Q000378:metabolism*; D053583:Interleukin-1beta / Q000378:metabolism*; D020935:MAP Kinase Signaling System / Q000187:drug effects*; D009000:Monocytes / Q000378:metabolism*; D008517:Phytotherapy; D010936:Plant Extracts / Q000302:isolation & purification* / Q000494:pharmacology* / Q000627:therapeutic use; D018515:Plant Leaves / Q000737:chemistry*; D011134:Polysaccharides / Q000302:isolation & purification* / Q000494:pharmacology*; D011493:Protein Kinase C / Q000037:antagonists & inhibitors*; D014867:Water
chemical_list: D000893:Anti-Inflammatory Agents; D018946:Chemokine CCL5; D018836:Inflammation Mediators; D053583:Interleukin-1beta; D010936:Plant Extracts; D011134:Polysaccharides; D014867:Water; D051546:Cyclooxygenase 2; C496540:PTGS2 protein, human; D011493:Protein Kinase C