title: [[Effect of lanthanum chloride on cytokines expression of RAW264.7 induced by alumina ceramic particles].
abstract: OBJECTIVE
  To investigate the relationship between alumina ceramic particles and aseptic loosening of the joint prosthesis and the effect of lanthanum chloride on the secretion of interleukin 1β (IL-1β) and tumor necrosis factor α (TNF-α) of macrophage RAW264.7 induced by alumina ceramic particles.
  
  
  METHODS
  RAW264.7 cells were cultured in vitro and divided randomly into 4 groups according to different culture solutions: blank control group (group A), 1 mg/mL alumina ceramic particles (group B), 1 mg/mL alumina ceramic particles and 10 μmol/L lanthanum chloride (group C), and 10 μmol/L lanthanum chloride (group D). The cell growth was detected by MTT, and ELISA, RT-PCR, and Western blot were used to test the expressions of IL-1β, TNF-α, and nuclear factor κB (NF-κB).
  
  
  RESULTS
  There was no significant difference in cell growth among all groups by MTT (F = 2.180, P = 0.142). RT-PCR results showed that the expressions of IL-1β, TNF-α, and NF-κB mRNA in group B were significantly higher than those in the other 3 groups (P < 0.05); the expressions in group D were significantly lower than those in group A (P < 0.05). ELISA results showed that the contents of IL-1β and TNF-α in group B were significantly higher than those in the other 3 groups (P < 0.05); the contents in group D were significantly lower than those in group A (P < 0.05). Western blot analysis revealed that the expression of NF-κB protein in group B was significantly higher than that in the other 3 groups (P < 0.05).
  
  
  CONCLUSIONS
  Alumina ceramic particles can stimulate the secretion of IL-1β and TNF-α of macrophage, and lanthanum chloride can inhibit the secretion of IL-1β and TNF-α of macrophage.
authors.forename: Tao
authors.initials: T
authors.lastname: Nie
authors.lastname: Dai Min
authors.forename: Chuang
authors.initials: C
authors.lastname: Jiang
authors.forename: Xiang
authors.initials: X
authors.lastname: Liu
mesh_terms: D000537:Aluminum Oxide / Q000494:pharmacology*; D001672:Biocompatible Materials / Q000737:chemistry; D015153:Blotting, Western; D016207:Cytokines / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005786:Gene Expression Regulation; D053583:Interleukin-1beta / Q000187:drug effects / Q000378:metabolism*; D007595:Joint Prosthesis; D007811:Lanthanum / Q000494:pharmacology*; D008264:Macrophages / Q000166:cytology; D016328:NF-kappa B / Q000187:drug effects / Q000378:metabolism*; D010316:Particle Size; D016133:Polymerase Chain Reaction / Q000379:methods; D011474:Prosthesis Design; D012333:RNA, Messenger / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014409:Tumor Necrosis Factor-alpha / Q000187:drug effects / Q000378:metabolism
chemical_list: D001672:Biocompatible Materials; D016207:Cytokines; D053583:Interleukin-1beta; D016328:NF-kappa B; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; C028521:lanthanum chloride; D007811:Lanthanum; D000537:Aluminum Oxide