title: Evaluation of zraP gene expression characteristics and construction of a lead (Pb) sensing and removal system in a recombinant Escherichia coli.
abstract: A ZraP-based lead sensing and removal system was constructed in E. coli. It was regulated by the ZraS/ZraR two-component system. The expression profile of the zraP gene towards extracellular lead was studied via real-time PCR. A dual-function bacterial system was also designed to express GFP and OmpC-lead binding peptide under the control of zraP for the simultaneous sensing and adsorption of environmental lead without additional manipulation. The constructed bacterial system can emit fluorescence and it adsorbed a maximum of 487 µmol lead/g cell DCW. From a study of artificial wastewater, the constructed bacteria adsorbed lead highly selectively (427 µmol lead/g cell DCW) among other metal ions. The newly-constructed dual function bacterial system can be applied for the development of an efficient process for the removal of lead from polluted wastes.
authors.affiliation: Department of Chemical Engineering, University of Ulsan, 93 Daehak-ro, Namgu, Ulsan, 680-749, Republic of Korea.
authors.forename: Murali Kannan
authors.initials: MK
authors.lastname: Maruthamuthu
authors.forename: Irisappan
authors.initials: I
authors.lastname: Ganesh
authors.forename: Sambandam
authors.initials: S
authors.lastname: Ravikumar
authors.forename: Soon Ho
authors.initials: SH
authors.lastname: Hong
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D004926:Escherichia coli / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism*; D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects*; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins / Q000032:analysis / Q000235:genetics; D007854:Lead / Q000378:metabolism*; D018272:Porins / Q000378:metabolism; D011485:Protein Binding; D060888:Real-Time Polymerase Chain Reaction; D062065:Wastewater / Q000737:chemistry; D018508:Water Purification
chemical_list: D029968:Escherichia coli Proteins; D049452:Green Fluorescent Proteins; D007854:Lead; D018272:Porins; D062065:Wastewater; C113724:OmpC protein; C114106:zraP protein, E coli