title: Detection of activated caspase-8 in injured spinal axons by using fluorochrome-labeled inhibitors of caspases (FLICA).
abstract: Here, we present a detailed protocol for the detection of activated caspase-8 in axotomized axons of the whole-mounted lamprey spinal cord. This method is based on the use of fluorochrome -labeled inhibitors of caspases (FLICA) in ex vivo tissue. We offer a very convenient vertebrate model to study the retrograde degeneration of descending pathways after spinal cord injury.
authors.affiliation: Centre for Neuroregeneration, School of Biomedical Sciences, University of Edinburgh, 49 Little France Crescent, Edinburgh, EH16 4SB, UK, anton.barreiro@gmail.com.
authors.forename: Antón
authors.initials: A
authors.lastname: Barreiro-Iglesias
authors.forename: Michael I
authors.initials: MI
authors.lastname: Shifman
mesh_terms: D000818:Animals; D001369:Axons / Q000201:enzymology* / Q000473:pathology; D053181:Caspase 8 / Q000235:genetics* / Q000302:isolation & purification; D061945:Caspase Inhibitors; D004195:Disease Models, Animal; D005456:Fluorescent Dyes; D007798:Lampreys; D008967:Molecular Biology / Q000379:methods; D013119:Spinal Cord Injuries / Q000175:diagnosis / Q000201:enzymology* / Q000473:pathology
chemical_list: D061945:Caspase Inhibitors; D005456:Fluorescent Dyes; D053181:Caspase 8