title: Fabrication of mineralized electrospun PLGA and PLGA/gelatin nanofibers and their potential in bone tissue engineering.
abstract: Surface mineralization is an effective method to produce calcium phosphate apatite coating on the surface of bone tissue scaffold which could create an osteophilic environment similar to the natural extracellular matrix for bone cells. In this study, we prepared mineralized poly(D,L-lactide-co-glycolide) (PLGA) and PLGA/gelatin electrospun nanofibers via depositing calcium phosphate apatite coating on the surface of these nanofibers to fabricate bone tissue engineering scaffolds by concentrated simulated body fluid method, supersaturated calcification solution method and alternate soaking method. The apatite products were characterized by the scanning electron microscopy (SEM), Fourier transform-infrared spectroscopy (FT-IR), and X-ray diffractometry (XRD) methods. A large amount of calcium phosphate apatite composed of dicalcium phosphate dihydrate (DCPD), hydroxyapatite (HA) and octacalcium phosphate (OCP) was deposited on the surface of resulting nanofibers in short times via three mineralizing methods. A larger amount of calcium phosphate was deposited on the surface of PLGA/gelatin nanofibers rather than PLGA nanofibers because gelatin acted as nucleation center for the formation of calcium phosphate. The cell culture experiments revealed that the difference of morphology and components of calcium phosphate apatite did not show much influence on the cell adhesion, proliferation and activity.
authors.affiliation: Center for Biomedical Materials and Engineering, Harbin Engineering University, Harbin 150001, China.
authors.forename: Z X
authors.initials: ZX
authors.lastname: Meng
authors.affiliation: Department of Materials Science and Engineering, College of Engineering, Peking University, Beijing 100871, China.
authors.forename: H F
authors.initials: HF
authors.lastname: Li
authors.affiliation: Center for Biomedical Materials and Engineering, Harbin Engineering University, Harbin 150001, China.
authors.forename: Z Z
authors.initials: ZZ
authors.lastname: Sun
authors.affiliation: Center for Biomedical Materials and Engineering, Harbin Engineering University, Harbin 150001, China. Electronic address: zhengwei@hrbeu.edu.cn.
authors.forename: W
authors.initials: W
authors.lastname: Zheng
authors.affiliation: Center for Biomedical Materials and Engineering, Harbin Engineering University, Harbin 150001, China; Department of Materials Science and Engineering, College of Engineering, Peking University, Beijing 100871, China. Electronic address: yfzheng@pku.edu.cn.
authors.forename: Y F
authors.initials: YF
authors.lastname: Zheng
mesh_terms: D001842:Bone and Bones / Q000166:cytology*; D002130:Calcium Phosphates / Q000737:chemistry; D002448:Cell Adhesion / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D005780:Gelatin / Q000737:chemistry* / Q000494:pharmacology; D006801:Humans; D019344:Lactic Acid / Q000737:chemistry* / Q000494:pharmacology; D057139:Nanofibers / Q000737:chemistry*; D036103:Nanotechnology / Q000379:methods; D011100:Polyglycolic Acid / Q000737:chemistry* / Q000494:pharmacology; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D002130:Calcium Phosphates; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D005780:Gelatin; C020243:calcium phosphate