title: miR-27 regulates mitochondrial networks by directly targeting the mitochondrial fission factor.
abstract: Mitochondrial morphology is dynamically regulated by forming small, fragmented units or interconnected networks, and this is a pivotal process that is used to maintain mitochondrial homeostasis. Although dysregulation of mitochondrial dynamics is related to the pathogenesis of several human diseases, its molecular mechanism is not fully elucidated. In this study, we demonstrate the potential role of miR-27 in the regulation of mitochondrial dynamics. Mitochondrial fission factor (MFF) mRNA is a direct target of miR-27, whose ectopic expression decreases MFF expression through binding to its 3'-untranslated region. Expression of miR-27 results in the elongation of mitochondria as well as an increased mitochondrial membrane potential and mitochondrial ATP level. Our results suggest that miR-27 is a novel regulator affecting morphological mitochondrial changes by targeting MFF.
authors.affiliation: Department of Biochemistry, College of Medicine, The Catholic University of Korea, Seoul, South Korea.
authors.forename: Hyosun
authors.initials: H
authors.lastname: Tak
authors.affiliation: Department of Molecular Science and Technology, Ajou University, Suwon, South Korea.
authors.forename: Jihye
authors.initials: J
authors.lastname: Kim
authors.affiliation: Department of Cellular and Molecular Medicine, College of Medicine, Chosun University, Gwangju, South Korea.
authors.forename: Aravinth Kumar
authors.initials: AK
authors.lastname: Jayabalan
authors.affiliation: Department of Biochemistry, College of Medicine, The Catholic University of Korea, Seoul, South Korea.
authors.forename: Heejin
authors.initials: H
authors.lastname: Lee
authors.affiliation: Department of Biochemistry, College of Medicine, The Catholic University of Korea, Seoul, South Korea.
authors.forename: Hoin
authors.initials: H
authors.lastname: Kang
authors.affiliation: Department of East-West Medical Science, Graduate School of East-West Medical Science, Kyung Hee University, Suwon, South Korea.
authors.forename: Dong-Hyung
authors.initials: DH
authors.lastname: Cho
authors.affiliation: Department of Cellular and Molecular Medicine, College of Medicine, Chosun University, Gwangju, South Korea.
authors.forename: Takbum
authors.initials: T
authors.lastname: Ohn
authors.affiliation: 1] Department of Pathology, College of Medicine, The Catholic University of Korea, Seoul, South Korea [2] Cancer Evolution Research Center, The Catholic University of Korea, Seoul, South Korea.
authors.forename: Suk Woo
authors.initials: SW
authors.lastname: Nam
authors.affiliation: Department of Molecular Science and Technology, Ajou University, Suwon, South Korea.
authors.forename: Wook
authors.initials: W
authors.lastname: Kim
authors.affiliation: 1] Department of Biochemistry, College of Medicine, The Catholic University of Korea, Seoul, South Korea [2] Cancer Evolution Research Center, The Catholic University of Korea, Seoul, South Korea.
authors.forename: Eun Kyung
authors.initials: EK
authors.lastname: Lee
mesh_terms: D020413:3' Untranslated Regions; D002460:Cell Line; D005786:Gene Expression Regulation; D006801:Humans; D053078:Membrane Potential, Mitochondrial; D008565:Membrane Proteins / Q000235:genetics*; D035683:MicroRNAs / Q000378:metabolism*; D008928:Mitochondria / Q000235:genetics* / Q000378:metabolism*; D063154:Mitochondrial Dynamics; D024101:Mitochondrial Proteins / Q000235:genetics*; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
chemical_list: D020413:3' Untranslated Regions; C531388:MIRN27 microRNA, human; D008565:Membrane Proteins; C533267:Mff protein, human; D035683:MicroRNAs; D024101:Mitochondrial Proteins; D012333:RNA, Messenger