title: Tuning the properties of mucin via layer-by-layer assembly.
abstract: Multilayer films consisting of bovine submaxillary mucin (BSM) and poly(allylamine hydrochloride) (PAH) were prepared on various substrates using layer-by-layer assembly. The effects of both the assembly pH and ionic strength on multilayer characteristics were investigated by assessing film thicknesses (10-80 nm), surface wetting characteristics, and cell repulsion. Also, the dynamic assembly behavior was monitored using quartz crystal microbalance with dissipation monitoring (QCM-D) to further understand the effect of assembly pH on film characteristics. Assembly studies revealed that substantial amounts of BSM adhere to the outermost surface only at low pH conditions. The resulting multilayer films assembled at low pH conditions were found to exhibit hydrophilic and cell repellent behavior. In addition, it was found that batch-to-batch variations of the biopolymer BSM could dramatically alter properties.
authors.affiliation: Department of Chemical Engineering, ‡Department of Biological Engineering, and §Department of Materials Science and Engineering, Massachusetts Institute of Technology , Cambridge, Massachusetts 02139, United States.
authors.forename: Jiyoung
authors.initials: J
authors.lastname: Ahn
authors.forename: Thomas
authors.initials: T
authors.lastname: Crouzier
authors.forename: Katharina
authors.initials: K
authors.lastname: Ribbeck
authors.forename: Michael F
authors.initials: MF
authors.lastname: Rubner
authors.forename: Robert E
authors.initials: RE
authors.lastname: Cohen
mesh_terms: D000327:Adsorption; D000818:Animals; D001704:Biopolymers / Q000737:chemistry; D002417:Cattle; D006367:HeLa Cells; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008567:Membranes, Artificial; D009077:Mucins / Q000737:chemistry*; D058525:Quartz Crystal Microbalance Techniques / Q000379:methods*; D013499:Surface Properties
chemical_list: D001704:Biopolymers; D008567:Membranes, Artificial; D009077:Mucins