title: Evidence of Oxidative Injury in Pigs With Melanosis Coli.
abstract: Melanosis coli is a dark discoloration of the colon due to accumulation of pigment-laden macrophages in the lamina propria. Three case submissions were received where rectal discoloration was reported at slaughter in pigs from separate production systems and melanosis coli was confirmed microscopically. Tissues from affected and unaffected cohort pigs were evaluated for evidence of oxidative damage using immunohistochemical staining for 3-nitrotyrosine, 4-hyroxynonenol, and malondialdehyde. Affected colons had significantly greater immunolabeling for all 3 target compounds than unaffected colons (P ≤ .001, all analyses). Hepatic vitamin E levels were low in both affected and unaffected pigs, and there was a trend toward lower values in affected pigs. Given the limited number of slaughter-collected samples available for this investigation, further study is warranted to elucidate the possible association between low vitamin E concentrations and oxidative damage in cases of melanosis coli in pigs.
authors.affiliation: Department of Veterinary Pathology, College of Veterinary Medicine, Iowa State University, Ames, IA, USA.
authors.forename: B L
authors.initials: BL
authors.lastname: Wilberts
authors.affiliation: Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA, USA.
authors.forename: K J
authors.initials: KJ
authors.lastname: Schwartz
authors.affiliation: Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA, USA.
authors.forename: P C
authors.initials: PC
authors.lastname: Gauger
authors.affiliation: Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA, USA.
authors.forename: C
authors.initials: C
authors.lastname: Wang
authors.affiliation: Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA, USA burrough@iastate.edu.
authors.forename: E R
authors.initials: ER
authors.lastname: Burrough
mesh_terms: D000447:Aldehydes / Q000378:metabolism; D000818:Animals; D003106:Colon / Q000473:pathology; D003108:Colonic Diseases / Q000473:pathology / Q000662:veterinary*; D005260:Female; D008264:Macrophages / Q000473:pathology; D008315:Malondialdehyde / Q000378:metabolism; D008548:Melanosis / Q000473:pathology / Q000662:veterinary*; D018384:Oxidative Stress; D013552:Swine; D014443:Tyrosine / Q000031:analogs & derivatives / Q000378:metabolism
chemical_list: D000447:Aldehydes; C002744:3-nitrotyrosine; D014443:Tyrosine; D008315:Malondialdehyde; C027576:4-hydroxy-2-nonenal
keywords: 3-nitrotyrosine; 4-hydroxynonenal; ceroid; lipofuscin; malondialdehyde; melanosis coli; pseudomelanosis coli