title: Bioactive Metabolites Produced by Pseudonocardia endophytica VUK-10 from Mangrove Sediments: Isolation, Chemical Structure Determination and Bioactivity.
abstract: Chemical investigation of the actinobacterial isolate Pseudonocardia endophytica VUK-10 has led to the segregation of two known bioactive compounds, namely 4-(2-acetamidoethyl) phenyl acetate and 4-((1, 4-dioxooctahydropyrrolo [1, 2-a] pyrazin-3-yl) methyl) phenyl acetate. The strain was isolated from a sediment sample of the Nizampatnam mangrove ecosystem, south coastal Andhra Pradesh, India. The chemical structure of the active compounds was established on the basis of spectroscopic analysis, including (1)H NMR and (13)C NMR spectroscopies, FTIR, and EIMS. The antimicrobial and cytotoxic activities of the bioactive compounds produced by the strain were tested against opportunistic and pathogenic bacteria and fungi and on MDA-MB-231, OAW, HeLa, and MCF-7 cell lines. The compounds exhibited antimicrobial activities against gram-positive and gram-negative bacteria and fungi and also showed potent cytotoxic activity against MDA-MB-231, OAW, HeLa, and MCF-7 cell lines. This is the first example for this class of bioactive compounds isolated from Pseudonocardia of mangrove origin.
authors.affiliation: Department of Botany and Microbiology, Acharya Nagarjuna University, Guntur-522510, Andhra Pradesh, India.
authors.forename: Usha Kiranmayi
authors.initials: UK
authors.lastname: Mangamuri
authors.affiliation: Department of Botany and Microbiology, Acharya Nagarjuna University, Guntur-522510, Andhra Pradesh, India.
authors.forename: Muvva
authors.initials: M
authors.lastname: Vijayalakshmi
authors.affiliation: Department of Biotechnology, Acharya Nagarjuna University, Guntur-522510, Andhra Pradesh, India.
authors.forename: Sudhakar
authors.initials: S
authors.lastname: Poda
authors.affiliation: Molecular and Cellular Oncology Laboratory, Department of Biochemistry, School of Life Sciences, University of Hyderabad, Hyderabad-500046, India.
authors.forename: Bramanandam
authors.initials: B
authors.lastname: Manavathi
authors.affiliation: Organic Chemistry Division-I, Indian Institute of Chemical Technology, Hyderabad-500007, India.
authors.forename: Ch
authors.initials: Ch
authors.lastname: Bhujangarao
authors.affiliation: Organic Chemistry Division-I, Indian Institute of Chemical Technology, Hyderabad-500007, India.
authors.forename: Y
authors.initials: Y
authors.lastname: Venkateswarlu
mesh_terms: D000192:Actinomycetales / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D001688:Biological Products / Q000494:pharmacology / Q000633:toxicity; D002470:Cell Survival / Q000187:drug effects; D005658:Fungi / Q000187:drug effects; D006367:HeLa Cells; D006801:Humans; D007194:India; D061986:MCF-7 Cells; D008826:Microbial Sensitivity Tests; D050296:Microbial Viability / Q000187:drug effects; D019906:Nuclear Magnetic Resonance, Biomolecular; D012988:Soil Microbiology; D053833:Wetlands
chemical_list: D001688:Biological Products