title: Facile characterization of aptamer kinetic and equilibrium binding properties using surface plasmon resonance.
abstract: Nucleic acid aptamers find widespread use as targeting and sensing agents in nature and biotechnology. Their ability to bind an extensive range of molecular targets, including small molecules, proteins, and ions, with high affinity and specificity enables their use in diverse diagnostic, therapeutic, imaging, and gene-regulatory applications. Here, we describe methods for characterizing aptamer kinetic and equilibrium binding properties using a surface plasmon resonance-based platform. This aptamer characterization platform is broadly useful for studying aptamer-ligand interactions, comparing aptamer properties, screening functional aptamers during in vitro selection processes, and prototyping aptamers for integration into nucleic acid devices.
authors.affiliation: Department of Chemistry, Stanford University, Stanford, California, USA.
authors.forename: Andrew L
authors.initials: AL
authors.lastname: Chang
authors.affiliation: Department of Bioengineering, Stanford University, Stanford, California, USA.
authors.forename: Maureen
authors.initials: M
authors.lastname: McKeague
authors.affiliation: Department of Bioengineering, Stanford University, Stanford, California, USA. Electronic address: csmolke@stanford.edu.
authors.forename: Christina D
authors.initials: CD
authors.lastname: Smolke
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry* / Q000378:metabolism; D001483:Base Sequence; D001665:Binding Sites; D015374:Biosensing Techniques / Q000379:methods; D007700:Kinetics; D008024:Ligands; D008969:Molecular Sequence Data; D052156:SELEX Aptamer Technique / Q000379:methods; D020349:Surface Plasmon Resonance / Q000379:methods*
chemical_list: D052157:Aptamers, Nucleotide; D008024:Ligands
keywords: aptamer; binding affinity; in vitro selection; small molecules; surface plasmon resonance