title: [Enzymatic production of α-ketoglutaric acid by L-glutamate oxidase from L-glutamic acid].
abstract: We produced α-ketoglutaric acid (α-KG) from L-glutamic acid, using enzymatic transformation approach with L-glutamate oxidase (LGOX). First, wild strain Streptomyces sp. FMME066 was mutated with NTG, a genetically stable mutant Streptomyces sp. FMME067 was obtained. Under the optimal nutrition conditions with fructose 10 g/L, peptone 7.5 g/L, KH2PO4 1 g/L and CaCl2 0.05 g/L, the maximum LGOX activity reached 0.14 U/mL. The LGOX was stable to pH and temperature, and Mn2+ had a stimulating effect. Finally, after 24 h enzymatic conversion under the optimal conditions, the maximum titer of α-KG reached 38.1 g/L from 47 g/L L-glutamic acid. Enzymatic transformation by LGOX is a potential approach for α-KG production.
authors.forename: Panqing
authors.initials: P
authors.lastname: Niu
authors.forename: Zhenyu
authors.initials: Z
authors.lastname: Zhang
authors.forename: Liming
authors.initials: L
authors.lastname: Liu
mesh_terms: D000594:Amino Acid Oxidoreductases / Q000378:metabolism*; D005285:Fermentation; D018698:Glutamic Acid / Q000378:metabolism*; D007656:Ketoglutaric Acids / Q000378:metabolism*; D013302:Streptomyces / Q000235:genetics / Q000378:metabolism
chemical_list: D007656:Ketoglutaric Acids; D018698:Glutamic Acid; D000594:Amino Acid Oxidoreductases; C023061:L-glutamate oxidase