title: A new type of toxin A-negative, toxin B-positive Clostridium difficile strain lacking a complete tcdA gene.
abstract: Toxins A and B are the main virulence factors of Clostridium difficile and are the targets for molecular diagnostic tests. Here, we describe a new toxin A-negative, toxin B-positive, binary toxin CDT (Clostridium difficile transferase)-negative (A(-) B(+) CDT(-)) toxinotype (XXXII) characterized by a variant type of pathogenicity locus (PaLoc) without tcdA and with atypical organization of the PaLoc integration site.
authors.affiliation: National Laboratory for Health, Environment and Food, Maribor, Slovenia sandra.janezic@nlzoh.si.
authors.forename: Sandra
authors.initials: S
authors.lastname: Janezic
authors.affiliation: Servicio de Microbiología Clínica-Enfermedades Infecciosas, Hospital General Universitario Gregorio Marańón, Madrid, Spain Instituto de Investigación Sanitaria Gregorio Marańón, Madrid, Spain.
authors.forename: Mercedes
authors.initials: M
authors.lastname: Marín
authors.affiliation: Servicio de Microbiología Clínica-Enfermedades Infecciosas, Hospital General Universitario Gregorio Marańón, Madrid, Spain Instituto de Investigación Sanitaria Gregorio Marańón, Madrid, Spain.
authors.forename: Adoración
authors.initials: A
authors.lastname: Martín
authors.affiliation: National Laboratory for Health, Environment and Food, Maribor, Slovenia University of Maribor, Faculty of Medicine, Maribor, Slovenia Centre of Excellence for Integrated Approaches in Chemistry and Biology of Proteins, Ljubljana, Slovenia.
authors.forename: Maja
authors.initials: M
authors.lastname: Rupnik
mesh_terms: D036002:ADP Ribose Transferases / Q000172:deficiency; D000368:Aged; D001426:Bacterial Proteins / Q000235:genetics*; D001427:Bacterial Toxins / Q000235:genetics*; D016360:Clostridioides difficile / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D016000:Cluster Analysis; D004768:Enterotoxins / Q000172:deficiency*; D023061:Gene Order; D044404:Genomic Islands; D005838:Genotype; D006801:Humans; D008297:Male; D021521:Ribotyping
chemical_list: D001426:Bacterial Proteins; D001427:Bacterial Toxins; D004768:Enterotoxins; C041348:tcdA protein, Clostridium difficile; C031815:toxB protein, Clostridium difficile; D036002:ADP Ribose Transferases; C410164:actin-specific ADP-ribosyltransferase, Clostridium