title: Alumina surfaces with nanoscale topography reduce attachment and biofilm formation by Escherichia coli and Listeria spp.
abstract: This work reports on a simple, robust and scientifically sound method to develop surfaces able to reduce microbial attachment and biofilm development, with possible applications in medicine, dentistry, food processing, or water treatment. Anodic surfaces with cylindrical nanopores 15 to 100 nm in diameter were manufactured and incubated with Escherichia coli ATCC 25922 and Listeria innocua. Surfaces with 15 and 25 nm pore diameters significantly repressed attachment and biofilm formation. Surface-bacteria interaction forces calculated using the extended Derjaguin Landau Verwey-Overbeek (XDLVO) theory indicate that reduction in attachment and biofilm formation is due to a synergy between electrostatic repulsion and surface effective free energy. An attachment study using E. coli K12 strains unable to express appendages also suggests that the small-pore surfaces may inhibit flagella-dependent attachment. These results can have immediate, far-reaching implications and commercial applications, with substantial benefits for human health and life.
authors.affiliation: a Department of Food Science , Cornell University , Ithaca , NY , USA.
authors.forename: Guoping
authors.initials: G
authors.lastname: Feng
authors.forename: Yifan
authors.initials: Y
authors.lastname: Cheng
authors.forename: Shu-Yi
authors.initials: SY
authors.lastname: Wang
authors.forename: Lillian C
authors.initials: LC
authors.lastname: Hsu
authors.forename: Yazmin
authors.initials: Y
authors.lastname: Feliz
authors.forename: Diana A
authors.initials: DA
authors.lastname: Borca-Tasciuc
authors.forename: Randy W
authors.initials: RW
authors.lastname: Worobo
authors.forename: Carmen I
authors.initials: CI
authors.lastname: Moraru
mesh_terms: D000537:Aluminum Oxide; D001422:Bacterial Adhesion; D018441:Biofilms / Q000254:growth & development*; D004926:Escherichia coli / Q000254:growth & development*; D005407:Flagella / Q000502:physiology; D008087:Listeria / Q000254:growth & development*; D018613:Microscopy, Confocal; D008855:Microscopy, Electron, Scanning; D008962:Models, Theoretical; D058608:Nanopores; D013499:Surface Properties
chemical_list: D000537:Aluminum Oxide
keywords: Escherichia coli; Listeria; bacterial attachment; biofilms; nanoscale anodized alumina