title: Regulation of anti-sense transcription by Mot1p and NC2 via removal of TATA-binding protein (TBP) from the 3'-end of genes.
abstract: The activity and dynamic nature of TATA-binding protein (TBP) crucial to RNA polymerase II-mediated transcription is under control of the Mot1p and NC2 complexes. Here we show that both TBP regulatory factors play 'hidden' roles in ensuring transcription fidelity by restricting anti-sense non-coding RNA (ncRNA) synthesis. Production of anti-sense ncRNA transcripts is suppressed by Mot1p- and NC2-mediated release of TBP from binding sites at the 3'-end of genes. In this, Mot1p and NC2 collaborate with the Nrd1p-Nab3p-Sen1p (NNS) complex that terminates the synthesis of anti-sense ncRNAs. In several cases anti-sense ncRNA expression interferes with expression of the cognate sense transcript. Our data reveal a novel regulatory mechanism to suppress anti-sense ncRNA expression and pre-initiation complex (PIC) formation at spurious sites.
authors.affiliation: Department of Molecular Cancer Research, Center for Molecular Medicine, University Medical Center Utrecht, 3584 CG, Utrecht, The Netherlands.
authors.forename: Maria J E
authors.initials: MJ
authors.lastname: Koster
authors.affiliation: Department of Molecular Cancer Research, Center for Molecular Medicine, University Medical Center Utrecht, 3584 CG, Utrecht, The Netherlands h.t.m.timmers@umcutrecht.nl.
authors.forename: H Th Marc
authors.initials: HT
authors.lastname: Timmers
mesh_terms: D024509:3' Flanking Region; D000251:Adenosine Triphosphatases / Q000378:metabolism*; D002843:Chromatin / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal; D010750:Phosphoproteins / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D016372:RNA, Antisense / Q000096:biosynthesis*; D022661:RNA, Untranslated / Q000096:biosynthesis; D016601:RNA-Binding Proteins / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D035381:TATA-Binding Protein Associated Factors / Q000378:metabolism*; D035181:TATA-Box Binding Protein / Q000378:metabolism*; D014157:Transcription Factors / Q000378:metabolism*; D014158:Transcription, Genetic
chemical_list: D002843:Chromatin; C491211:NRD1 protein, S cerevisiae; D010750:Phosphoproteins; D016372:RNA, Antisense; D022661:RNA, Untranslated; D016601:RNA-Binding Proteins; C115017:SPT15 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D035381:TATA-Binding Protein Associated Factors; D035181:TATA-Box Binding Protein; D014157:Transcription Factors; C075995:down-regulator of transcription 1; D000251:Adenosine Triphosphatases; C073676:MOT1 protein, S cerevisiae