title: Response of periodontitis and healthy patients in a Porphyromonas gingivalis-stimulated whole-blood model.
abstract: OBJECTIVE
  To investigate the inflammatory responses of periodontitis patients and healthy patients in a whole-blood model stimulated with Porphyromonas gingivalis.
  
  
  METHODS
  Whole blood collected from 17 periodontitis patients and six healthy patients was stimulated with Porphyromonas gingivalis cells. The secretion of cytokines and matrix metalloproteinases was quantified by enzyme-linked immunosorbent assay. An analysis of covariance with the ancova model was used to evaluate the significance of differences in secreted host molecules by whole blood from the periodontitis and healthy groups.
  
  
  RESULTS
  Porphyromonas gingivalis induced the secretion of interleukin-1β, interleukin-6, interleukin-8, tumor necrosis factor-α, monocyte chemoattractant protein-1, interferon inducible protein-10 by whole blood from patients in the periodontitis and healthy groups. Matrix metalloproteinase-8 and -9 levels secreted by whole blood also increased following stimulation. No significant differences (P < 0.05) in the amounts of secreted host molecules were observed between periodontitis and healthy patients.
  
  
  CONCLUSIONS
  This study suggests that Porphyromonas gingivalis can provoke an inflammatory response and promote the progression of periodontitis by inducing the secretion of high levels of cytokines and matrix metalloproteinases by a mixed leukocyte population. However, the whole-blood model did not reveal any significant differences in the inflammatory response between periodontitis patients (n = 17) and periodontally-healthy patients (n = 6).
authors.affiliation: Oral Ecology Research Group, Faculty of Dentistry, Laval University, Quebec City, Canada.
authors.forename: Daniel
authors.initials: D
authors.lastname: Grenier
authors.forename: Julia
authors.initials: J
authors.lastname: Cazalis
authors.forename: Guy
authors.initials: G
authors.lastname: Gagnon
mesh_terms: D018932:Chemokine CCL2 / Q000097:blood; D018946:Chemokine CCL5 / Q000097:blood; D054357:Chemokine CXCL10 / Q000097:blood; D018450:Disease Progression; D006801:Humans; D053583:Interleukin-1beta / Q000097:blood; D015850:Interleukin-6 / Q000097:blood; D016209:Interleukin-8 / Q000097:blood; D007962:Leukocytes / Q000276:immunology; D020784:Matrix Metalloproteinase 8 / Q000097:blood; D020780:Matrix Metalloproteinase 9 / Q000097:blood; D017622:Periodontal Attachment Loss / Q000097:blood / Q000382:microbiology; D010514:Periodontal Pocket / Q000097:blood / Q000382:microbiology; D010518:Periodontitis / Q000097:blood / Q000382:microbiology*; D016966:Porphyromonas gingivalis / Q000276:immunology*; D014409:Tumor Necrosis Factor-alpha / Q000097:blood
chemical_list: C111835:CCL2 protein, human; C483024:CCL5 protein, human; C508606:CXCL8 protein, human; D018932:Chemokine CCL2; D018946:Chemokine CCL5; D054357:Chemokine CXCL10; C508600:IL6 protein, human; D053583:Interleukin-1beta; D015850:Interleukin-6; D016209:Interleukin-8; D014409:Tumor Necrosis Factor-alpha; C488762:MMP8 protein, human; D020784:Matrix Metalloproteinase 8; C579270:MMP9 protein, human; D020780:Matrix Metalloproteinase 9
keywords: Porphyromonas gingivalis; cytokine; inflammatory response; matrix metalloproteinase; periodontitis