title: Nutrient starvation affects expression of LC3 family at the feto-maternal interface during murine placentation.
abstract: LC3 - the mammalian homolog of Atg8 - was found as autophagosome membrane binding protein in mammals and widely used as an autophagosomal marker. LC3A, B and C show different expression patterns in each tissue. The aim of this study was to reveal the differences of expression patterns among LC3 families in mouse placenta under normal condition and nutrient starving condition. LC3A and B were highly expressed in decidual cells. LC3A and B were increased in D14 compared with D12 and D16 in mouse placenta, while LC3C was decreased. Starvation induced increase in LC3B expression specifically. Immunohistochemistry showed different expression patterns among LC3A, B and C. LC3A expression in syncytiotrophoblast was vanished by starvation. The results of real time RT-PCR suggested differences between D12 and D16 in autophagic cascade induced by starvation. Taken together, this study suggests that autophagy could play a role in placental invasion system and that nutrient starvation affects LC3B expression.
authors.affiliation: Laboratory of Basic Veterinary Science, The United Graduate School of Veterinary Science, Yamaguchi University, Yamaguchi 753-8515, Japan.
authors.forename: Masato
authors.initials: M
authors.lastname: Hiyama
authors.forename: Ken Takeshi
authors.initials: KT
authors.lastname: Kusakabe
authors.forename: Ai
authors.initials: A
authors.lastname: Takeshita
authors.forename: Sinji
authors.initials: S
authors.lastname: Sugi
authors.forename: Nobue
authors.initials: N
authors.lastname: Kuniyoshi
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Imai
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Kano
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Kiso
mesh_terms: D000818:Animals; D001343:Autophagy / Q000502:physiology*; D005260:Female; D005786:Gene Expression Regulation / Q000502:physiology; D051379:Mice; D008813:Mice, Inbred ICR; D008869:Microtubule-Associated Proteins / Q000235:genetics / Q000378:metabolism*; D005810:Multigene Family; D010929:Placentation / Q000502:physiology*; D011247:Pregnancy
chemical_list: C480860:Map1lc3b protein, mouse; D008869:Microtubule-Associated Proteins