title: Metabolic pathway for degradation of 2-chloro-4-aminophenol by Arthrobacter sp. SPG.
abstract: A degradation pathway of 2-chloro-4-aminophenol (2C4AP) was studied in an Arthrobacter sp. SPG that utilized 2C4AP as its sole source of carbon and energy. The 2C4AP degradation was initiated by a 2C4AP-deaminase that catalyzed the conversion of 2C4AP into chlorohydroquinone (CHQ) with removal of ammonium ion. In the next step, a CHQ-dehalogenase dehalogenated CHQ to hydroquinone (HQ) that cleaved into γ-hydroxymuconic semialdehyde by a HQ-dioxygenase. The 2C4AP degradation was also investigated in sterile and non-sterile soil microcosms using strain SPG. The results show that the SPG cells degraded 2C4AP more rapidly in sterile soil than non-sterile soil. Our studies showed that strain SPG may be used for bioremediation of 2C4AP-contaminated sites. This is the first report of the 2C4AP degradation by any bacteria.
authors.affiliation: School of Biotechnology, Yeungnam University, Gyeongsan, 712-749, Republic of Korea. arora484@gmail.com.
authors.forename: Pankaj Kumar
authors.initials: PK
authors.lastname: Arora
authors.affiliation: School of Biotechnology, Yeungnam University, Gyeongsan, 712-749, Republic of Korea. nostoc.tapan@gmail.com.
authors.forename: Tapan Kumar
authors.initials: TK
authors.lastname: Mohanta
authors.affiliation: Department of Plant Science, Faculty of Applied Sciences, MJP Rohilkhand University, Bareilly, 243006, India. alok.plsc@rediffmail.com.
authors.forename: Alok
authors.initials: A
authors.lastname: Srivastava
authors.affiliation: School of Biotechnology, Yeungnam University, Gyeongsan, 712-749, Republic of Korea. hanhongbae@ynu.ac.kr.
authors.forename: Hanhong
authors.initials: H
authors.lastname: Bae
authors.affiliation: Department of Plant Science, Faculty of Applied Sciences, MJP Rohilkhand University, Bareilly, 243006, India. singhvp03@rediffmail.com.
authors.forename: Vijay Pal
authors.initials: VP
authors.lastname: Singh
mesh_terms: D000619:Aminohydrolases / Q000378:metabolism; D001173:Arthrobacter / Q000378:metabolism*; D001426:Bacterial Proteins / Q000378:metabolism; D001673:Biodegradation, Environmental; D002733:Chlorophenols / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D002733:Chlorophenols; C027296:2-amino-4-chlorophenol; D000619:Aminohydrolases