title: Alkaloids from beach spider lily (Hymenocallis littoralis) induce apoptosis of HepG-2 cells by the fas-signaling pathway.
abstract: Alkaloids are the most extensively featured compounds of natural anti-tumor herbs, which have attracted much attention in pharmaceutical research. In our previous studies, a mixture of major three alkaloid components (5, 6-dihydrobicolorine, 7-deoxy-trans-dihydronarciclasine, littoraline) from Hymenocallis littoralis were extracted, analyzed and designated as AHL. In this paper, AHL extracts were added to human liver hepatocellular cells HepG-2, human gastric cancer cell SGC-7901, human breast adenocarcinoma cell MCF-7 and human umbilical vein endothelial cell EVC-304, to screen one or more AHL-sensitive tumor cell. Among these cells, HepG-2 was the most sensitive to AHL treatment, a very low dose (0.8μg/ml) significantly inhibiting proliferation . The non- tumor cell EVC-304, however, was not apparently affected. Effect of AHL on HepG-2 cells was then explored. We found that the AHL could cause HepG-2 cycle arrest at G2/M checkpoint, induce apoptosis, and interrupt polymerization of microtubules. In addition, expression of two cell cycle-regulated proteins, CyclinB1 and CDK1, was up-regulated upon AHL treatment. Up-regulation of the Fas, Fas ligand, Caspase-8 and Caspase-3 was observed as well, which might imply roles for the Fas/FsaL signaling pathway in the AHL-induced apoptosis of HepG-2 cells.
authors.affiliation: Engineering Research Center of Natural Anticancer Drugs, Harbin University of Commerce, Harbin, China E-mail : chenning1981@126.com; zhuhongwei@caas.cn.
authors.forename: Yu-Bin
authors.initials: YB
authors.lastname: Ji
authors.forename: Ning
authors.initials: N
authors.lastname: Chen
authors.forename: Hong-Wei
authors.initials: HW
authors.lastname: Zhu
authors.forename: Na
authors.initials: N
authors.lastname: Ling
authors.forename: Wen-Lan
authors.initials: WL
authors.lastname: Li
authors.forename: Dong-Xue
authors.initials: DX
authors.lastname: Song
authors.forename: Shi-Yong
authors.initials: SY
authors.lastname: Gao
authors.forename: Wang-Cheng
authors.initials: WC
authors.lastname: Zhang
authors.forename: Nan-Nan
authors.initials: NN
authors.lastname: Ma
mesh_terms: D000470:Alkaloids / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects* / Q000235:genetics; D015153:Blotting, Western; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival; D053222:Fas Ligand Protein / Q000187:drug effects* / Q000235:genetics; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D056945:Hep G2 Cells / Q000166:cytology / Q000187:drug effects; D006801:Humans; D019668:Liliaceae; D010936:Plant Extracts; D012016:Reference Values; D012680:Sensitivity and Specificity; D015398:Signal Transduction / Q000187:drug effects* / Q000235:genetics; D014407:Tumor Cells, Cultured
chemical_list: D000470:Alkaloids; D053222:Fas Ligand Protein; D010936:Plant Extracts