title: Dynamic gene expressions of peripheral blood mononuclear cells in patients with acute exacerbation of chronic obstructive pulmonary disease: a preliminary study.
abstract: BACKGROUND
  Acute exacerbation of chronic obstructive pulmonary disease (AECOPD) is a serious event that is responsible for the progress of the disease, increases in medical costs and high mortality.
  
  
  METHODS
  The aim of the present study was to identify AECOPD-specific biomarkers by evaluating the dynamic gene expression profiling of peripheral blood mononuclear cells (PBMCs) from patients with AECOPD on days 1, 3 and 10 after hospital admission and to compare the derived data with data from healthy controls or patients with stable COPD.
  
  
  RESULTS
  We found that 14 genes were co-differentially upregulated and 2 downregulated greater than 10-fold in patients with COPD or AECOPD compared with the healthy individuals. Eight co-differentially upregulated genes and six downregulated genes were identified as a panel of AECOPD-specific genes. Downregulation of TCF7 in PBMCs was found to be associated with the severity of COPD. Dynamic changes of Aminolevulinate-delta-synthase 2 and carbonic anhydrase I had similar patterns of Digital Evaluation Score System scores and may serve as potential genes of interest during the course of AECOPD.
  
  
  CONCLUSIONS
  Thus, our findings indicate a panel of altered gene expression patterns in PBMCs that can be used as AECOPD-specific dynamic biomarkers to monitor the course of AECOPD.
authors.affiliation: Department of Respiratory Medicine, Zhongshan Hospital, Fudan University, Fenglin Rd. No180, 200032, Shanghai, China. physicianwuxd@126.com.
authors.forename: Xiaodan
authors.initials: X
authors.lastname: Wu
authors.affiliation: Department of Respiratory Medicine, Wenzhou Medical University and The First Hospital, Nanbaixiang, 325000, Wenzhou, China. sunxiaoru@outlook.com.
authors.forename: Xiaoru
authors.initials: X
authors.lastname: Sun
authors.affiliation: Department of Respiratory Medicine, Wenzhou Medical University and The First Hospital, Nanbaixiang, 325000, Wenzhou, China. chenchengshui@gmail.com.
authors.forename: Chengshui
authors.initials: C
authors.lastname: Chen
authors.affiliation: Shanghai Institute of Clinical Bioinformatics, Fudan University Center for Clinical Bioinformatics, Shanghai Respiratory Research Medicine, Fenglin Rd. No 180, 200032, Shanghai, China. bai.chunxue@zs-hospital.sh.cn.
authors.forename: Chunxue
authors.initials: C
authors.lastname: Bai
authors.affiliation: Department of Respiratory Medicine, Wenzhou Medical University and The First Hospital, Nanbaixiang, 325000, Wenzhou, China. xiangdong.wang@clintransmed.org.|Shanghai Institute of Clinical Bioinformatics, Fudan University Center for Clinical Bioinformatics, Shanghai Respiratory Research Medicine, Fenglin Rd. No 180, 200032, Shanghai, China. xiangdong.wang@clintransmed.org.
authors.forename: Xiangdong
authors.initials: X
authors.lastname: Wang
mesh_terms: D000208:Acute Disease; D000368:Aged; D016022:Case-Control Studies; D005260:Female; D020869:Gene Expression Profiling / Q000379:methods*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D029424:Pulmonary Disease, Chronic Obstructive / Q000097:blood* / Q000175:diagnosis / Q000235:genetics*