title: 1p36 deletion is a marker for tumour dissemination in microsatellite stable stage II-III colon cancer.
abstract: BACKGROUND
  The clinical behaviour of colon cancer is heterogeneous. Five-year overall survival is 50-65% with all stages included. Recurring somatic chromosomal alterations have been identified and some have shown potential as markers for dissemination of the tumour, which is responsible for most colon cancer deaths. We investigated 115 selected stage II-IV primary colon cancers for associations between chromosomal alterations and tumour dissemination.
  
  
  METHODS
  Follow-up was at least 5 years for stage II-III patients without distant recurrence. Affymetrix SNP 6.0 microarrays and allele-specific copy number analysis were used to identify chromosomal alterations. Fisher's exact test was used to associate alterations with tumour dissemination, detected at diagnosis (stage IV) or later as recurrent disease (stage II-III).
  
  
  RESULTS
  Loss of 1p36.11-21 was associated with tumour dissemination in microsatellite stable tumours of stage II-IV (odds ratio = 5.5). It was enriched to a similar extent in tumours with distant recurrence within stage II and stage III subgroups, and may therefore be used as a prognostic marker at diagnosis. Loss of 1p36.11-21 relative to average copy number of the genome showed similar prognostic value compared to absolute loss of copies. Therefore, the use of relative loss as a prognostic marker would benefit more patients by applying also to hyperploid cancer genomes. The association with tumour dissemination was supported by independent data from the The Cancer Genome Atlas.
  
  
  CONCLUSIONS
  Deletions on 1p36 may be used to guide adjuvant treatment decisions in microsatellite stable colon cancer of stages II and III.
authors.forename: Markus
authors.initials: M
authors.lastname: Mayrhofer
authors.forename: Hanna Göransson
authors.initials: HG
authors.lastname: Kultima
authors.forename: Helgi
authors.initials: H
authors.lastname: Birgisson
authors.forename: Magnus
authors.initials: M
authors.lastname: Sundström
authors.forename: Lucy
authors.initials: L
authors.lastname: Mathot
authors.forename: Karolina
authors.initials: K
authors.lastname: Edlund
authors.forename: Björn
authors.initials: B
authors.lastname: Viklund
authors.forename: Tobias
authors.initials: T
authors.lastname: Sjöblom
authors.forename: Johan
authors.initials: J
authors.lastname: Botling
authors.forename: Patrick
authors.initials: P
authors.lastname: Micke
authors.forename: Lars
authors.initials: L
authors.lastname: Påhlman
authors.forename: Bengt
authors.initials: B
authors.lastname: Glimelius
authors.affiliation: Science for Life Laboratory, Department of Medical Sciences, Uppsala University, Box 3056, Uppsala 750 03, Sweden. anders.isaksson@medsci.uu.se.
authors.forename: Anders
authors.initials: A
authors.lastname: Isaksson
mesh_terms: D014408:Biomarkers, Tumor / Q000235:genetics; D002872:Chromosome Deletion; D058674:Chromosome Duplication; D002878:Chromosomes, Human, Pair 1; D003110:Colonic Neoplasms / Q000235:genetics* / Q000401:mortality / Q000473:pathology*; D056915:DNA Copy Number Variations; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D008297:Male; D053842:Microsatellite Instability; D018895:Microsatellite Repeats / Q000235:genetics*; D060787:Neoplasm Grading; D009367:Neoplasm Staging; D011379:Prognosis; D047368:Tumor Burden
chemical_list: D014408:Biomarkers, Tumor