title: Comparative study of conductometric glucose biosensor based on gold and on magnetic nanoparticles.
abstract: The aim of this study was to show the feasibility and the performances of nanoparticle biosensing. A glucose conductometric biosensor was developed using two types of nanoparticles (gold and magnetic), glucose oxidase (GOD) being adsorbed on PAH (poly(allylamine hydrochloride)) modified nanoparticles, deposited on a planar interdigitated electrode (IDEs). The best sensitivities for glucose detection were obtained with magnetic nanoparticles (70 μM/mM and 3 μM of detection limit) compared to 45 μM/mM and 9 μM with gold nanoparticles and 30 μM/mM and 50 μM with GOD directly cross-linked on IDEs. When stored in phosphate buffer (20 mM, pH 7.3) at 4 °C, the biosensor showed good stability for more than 12 days.
authors.affiliation: Université de Lyon, Institut des Sciences Analytiques: Université Claude Bernard, Lyon 1, 43 Boulevard du 11 Novembre 1918, 69622 Villeurbanne, France; Laboratoire des Interfaces et Matériaux Avancés, Université de Monastir, Avenue de l'Environnement, 5019 Monastir, Tunisia. widednouira@yahoo.fr
authors.forename: Wided
authors.initials: W
authors.lastname: Nouira
authors.affiliation: Laboratoire des Interfaces et Matériaux Avancés, Université de Monastir, Avenue de l'Environnement, 5019 Monastir, Tunisia.
authors.forename: Abderrazak
authors.initials: A
authors.lastname: Maaref
authors.affiliation: Université de Lyon, Laboratoire LAGEP CPE-308G: Université Claude Bernard Lyon-1, 69622 Villeurbanne Cedex, France.
authors.forename: Hamid
authors.initials: H
authors.lastname: Elaissari
authors.affiliation: Université de Lyon, Laboratoire Hubert Curien, Université de Saint-Etienne Jean-Monnet, F-42023 Saint-Etienne, France.
authors.forename: Francis
authors.initials: F
authors.lastname: Vocanson
authors.affiliation: LASC, ISEA, Université de Metz, 7 rue Marconi, 57070 Metz, France.
authors.forename: Maryam
authors.initials: M
authors.lastname: Siadat
authors.affiliation: Université de Lyon, Institut des Sciences Analytiques: Université Claude Bernard, Lyon 1, 43 Boulevard du 11 Novembre 1918, 69622 Villeurbanne, France. Electronic address: nicole.jaffrezic@univ-lyon1.fr.
authors.forename: Nicole
authors.initials: N
authors.lastname: Jaffrezic-Renault
mesh_terms: D015374:Biosensing Techniques; D003217:Conductometry; D004566:Electrodes; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism; D005947:Glucose / Q000032:analysis*; D005949:Glucose Oxidase / Q000737:chemistry / Q000378:metabolism; D006046:Gold / Q000737:chemistry*; D058185:Magnetite Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D046529:Microscopy, Electron, Transmission; D011073:Polyamines / Q000737:chemistry
chemical_list: D004800:Enzymes, Immobilized; D058185:Magnetite Nanoparticles; D011073:Polyamines; C063994:polyallylamine; D006046:Gold; D005949:Glucose Oxidase; D005947:Glucose