title: Deciphering protein-protein interactions during the biogenesis of cytochrome c oxidase from Paracoccus denitrificans.
abstract: Biogenesis of the mitochondrial cytochrome c oxidase (COX) is a complex process due to its numerous subunits encoded by two genomes, as well as the localization of redox centers deep within the membrane. Here, we have assessed the biogenesis of the homologous aa₃-type oxidase of the soil bacterium Paracoccus denitrificans. First, protein partners were analyzed using various membrane solubilization strategies to show interactions between COX and CtaG, a chaperone implicated in CuB site metallation. Using an unbiased MS approach after immunological pull-down from untreated or cross-linked membranes, we then extend our view towards a hypothetical 'biogenesis complex' by identifying two further metal-inserting chaperones, Surf1c and Sco, together with enzymes catalyzing heme a synthesis. Our study also tentatively supports previous speculation regarding the existence of a predominantly co-translational mechanism for cofactor insertion during COX biogenesis.
authors.affiliation: Institute of Biochemistry, Molecular Genetics, Goethe University, Frankfurt am Main, Germany.
authors.forename: Priya
authors.initials: P
authors.lastname: Gurumoorthy
authors.forename: Bernd
authors.initials: B
authors.lastname: Ludwig
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism*; D003576:Electron Transport Complex IV / Q000378:metabolism*; D010231:Paracoccus denitrificans / Q000378:metabolism*; D011485:Protein Binding
chemical_list: D001426:Bacterial Proteins; D003576:Electron Transport Complex IV
keywords: CtaG; biogenesis complex; co-translational insertion; copper center; heme a synthesis