title: Immunosuppressive Effect of Hypodermin C on Complement Component 3 In Vitro.
abstract: Hypodermins A (HA), B (HB) and C (HC) are the major proteases secreted by first-instar larvae of Hypoderma lineatum (Diptera: Oestridae). These proteases are involved in the larval migration in the tissue, and prevent the activation of the host immune response. We previously showed that the recombinant HA functions as an immunosuppressive agent which could inhibit the rejection of xenotransplants. In the current study, we cloned the cDNA sequence of HC, which was transfected in Cos7 cells using the pEF1α-IRES-AcGFP expression vector. The Cos7 cells stably expressed HC, and were more resistant to lysis by guinea pig C3 than the control cells. The HC protease degraded the guinea pig C3, and inhibited the complement pathway in vitro. The DNA binding sites of HC were identified using an electrophoretic mobility shift assay. Our findings suggest that the recombinant HC might be useful as an immunosuppressive agent for the inhibition of the xenotransplant rejection.
authors.affiliation: College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu, China.|Laboratory Animal Center, Xuzhou Medical College, Xuzhou, Jiangsu, China.
authors.forename: An-Kang
authors.initials: AK
authors.lastname: Hu
authors.affiliation: Laboratory Animal Center, Xuzhou Medical College, Xuzhou, Jiangsu, China.
authors.forename: Ren-Jin
authors.initials: RJ
authors.lastname: Chen
authors.affiliation: Laboratory Animal Center, Xuzhou Medical College, Xuzhou, Jiangsu, China.
authors.forename: Xiao-Rong
authors.initials: XR
authors.lastname: Zhu
authors.affiliation: College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu, China.
authors.forename: Jian-Hong
authors.initials: JH
authors.lastname: Gu
authors.affiliation: College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu, China. liuzongping@yzu.edu.cn.|Laboratory Animal Center, Xuzhou Medical College, Xuzhou, Jiangsu, China. liuzongping@yzu.edu.cn.
authors.forename: Zong-Ping
authors.initials: ZP
authors.lastname: Liu
mesh_terms: D000818:Animals; D001665:Binding Sites; D019556:COS Cells; D002522:Chlorocebus aethiops; D003176:Complement C3 / Q000737:chemistry*; D003165:Complement System Proteins; D004175:Diptera / Q000201:enzymology; D004797:Enzyme-Linked Immunosorbent Assay; D006084:Graft Rejection; D006168:Guinea Pigs; D007107:Immune System; D007166:Immunosuppressive Agents / Q000494:pharmacology*; D007814:Larva / Q000201:enzymology; D016133:Polymerase Chain Reaction; D011994:Recombinant Proteins / Q000494:pharmacology; D012697:Serine Endopeptidases / Q000494:pharmacology*; D014162:Transfection; D014183:Transplantation, Heterologous
chemical_list: D003176:Complement C3; D007166:Immunosuppressive Agents; D011994:Recombinant Proteins; D003165:Complement System Proteins; D012697:Serine Endopeptidases; C070019:hypodermin C
keywords: Complement C3; Hypodermin C; Xenogeneic rejection