title: Protein characterisation of Brosimum gaudichaudii Trécul latex and study of nanostructured latex film formation.
abstract: Brosimum gaudichaudii Tréc. (Moraceae) is a common Brazilian Cerrado plant known by its pharmaceutical industry relevance. The authors investigated the latex protein components and potential biotechnological applications. Some protein fragments had their sequences elucidated, presenting similarities to jacalin and Kunitz-type trypsin inhibitors. Amino acid residue modifications were found, such as glutamine N-terminal residue cyclisation into pyroglutamic acid residue, and mass differences corresponding to hexoses and N-acetylhexosamine presence. The latex was used to produce a nanoscale structured film, which presented an increased attraction and reduced adhesion behaviours. The film presented high homogeneity, as observed by low nanoroughness values, probably because of its intrinsic components, such as the jacalin-like protein that has known agglutination properties. The immobilised Kunitz-type trypsin inhibitor presence in the latex film allow us to point out to applications related to this inhibition, as in active food packaging, since these peptidase inhibitors are able to inhibit pests and microorganism proliferation.
authors.affiliation: Postgraduate Program in Animal Biology, Institute of Biological Sciences, University of Brasília, UnB, Brasília, Brazil.
authors.forename: Eduardo F
authors.initials: EF
authors.lastname: Barbosa
authors.affiliation: Postgraduate Program in Animal Biology, Institute of Biological Sciences, University of Brasília, UnB, Brasília, Brazil.
authors.forename: Victoria
authors.initials: V
authors.lastname: Monge-Fuentes
authors.affiliation: Postgraduate Program in Animal Biology, Institute of Biological Sciences, University of Brasília, UnB, Brasília, Brazil.
authors.forename: Natiela B
authors.initials: NB
authors.lastname: Oliveira
authors.affiliation: Postgraduate Program in Animal Biology, Institute of Biological Sciences, University of Brasília, UnB, Brasília, Brazil.
authors.forename: Rebecca
authors.initials: R
authors.lastname: Tavares
authors.affiliation: Postgraduate Program in Animal Biology, Institute of Biological Sciences, University of Brasília, UnB, Brasília, Brazil.
authors.forename: Mary-Ann E
authors.initials: MA
authors.lastname: Xavier
authors.affiliation: Laboratory of Mass Spectrometry, Embrapa Genetic Resources and Biotechnology, Brasília, Brazil.
authors.forename: Marcelo Porto
authors.initials: MP
authors.lastname: Bemquerer
authors.affiliation: Postgraduate Program in Animal Biology, Institute of Biological Sciences, University of Brasília, UnB, Brasília, Brazil. lucianopaulinosilva@gmail.com.
authors.forename: Luciano P
authors.initials: LP
authors.lastname: Silva
mesh_terms: D000595:Amino Acid Sequence; D055119:Elastic Modulus; D007840:Latex / Q000737:chemistry*; D008969:Molecular Sequence Data; D029586:Moraceae / Q000737:chemistry*; D049329:Nanostructures / Q000737:chemistry*; D010455:Peptides; D037121:Plant Lectins; D010940:Plant Proteins / Q000737:chemistry*; D016415:Sequence Alignment
chemical_list: C053865:Kunitz-type protease inhibitor, plant; D007840:Latex; D010455:Peptides; D037121:Plant Lectins; D010940:Plant Proteins; C043696:jacalin