title: Quantification of fluorescent reporters in plant cells.
abstract: Fluorescent reporters are powerful tools for plant research. Many studies require accurate determination of fluorescence intensity and localization. Here, we describe protocols for the quantification of fluorescence intensity in plant cells from confocal laser scanning microscope images using semiautomated software and image analysis techniques.
authors.affiliation: Centre for Plant Integrative Biology, School of Biosciences, University of Nottingham, Sutton Bonington, LE12 5RD, UK.
authors.forename: Michael
authors.initials: M
authors.lastname: Pound
authors.forename: Andrew P
authors.initials: AP
authors.lastname: French
authors.forename: Darren M
authors.initials: DM
authors.lastname: Wells
mesh_terms: D017360:Arabidopsis / Q000737:chemistry / Q000166:cytology*; D005456:Fluorescent Dyes; D007091:Image Processing, Computer-Assisted / Q000379:methods; D047569:Laser Scanning Cytometry / Q000379:methods*; D008856:Microscopy, Fluorescence / Q000379:methods; D059828:Plant Cells / Q000737:chemistry*; D018517:Plant Roots / Q000737:chemistry / Q000166:cytology; D012984:Software
chemical_list: D005456:Fluorescent Dyes