title: Essential oil of Eucalyptus gunnii hook. As a novel source of antioxidant, antimutagenic and antibacterial agents.
abstract: The present study describes radical scavenging capacity (RSC), antimutagenic and antibacterial properties of the essential oil (EO) of the leaves of Eucalyptus gunnii Hook. (Southern Montenegro). Chemical composition was evaluated by gas chromatography-mass spectrometry (GC-MS). In oil, 1,8-cineole (67.8%) and α-pinene (14.12%) were the major compounds comprising almost 82% of total EO. EO exhibited moderate DPPH (2,2-diphenyl-1-picrylhydrazyl) scavenging activity, with IC50 value of 7.19 µL/mL. The antimutagenic properties were assayed against the spontaneous and t-BOOH-induced mutagenesis in Escherichia coli IC202 oxyR mutant strain, deficient in removing radical oxygen species (ROS). Reduction of the spontaneous mutagenesis in the presence of E. gunnii EO was only slight, up to 12% at the highest concentration tested. However, when the oxidative mutagen was used, EO displayed more significant reduction of mutagenesis (maximum 23%) in a concentration dependent manner. Antibacterial activity was tested against the selected strains from ATTC and NCIB collections: Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas aeruginosa, Escherichia coli, Bacillus subtilis, Micrococcus flavus, Klebsiella pneumoniae, and the two Escherichia coli strains from our laboratory collection (SY252 and IB112) using both the disk-diffusion and MIC assays. The greatest sensitivity was shown by M. flavus, K. pneumoniae and E. coli lpcA (MIC = 0.83 mg/mL), while the highest resistance was shown by E. coli (ATTC 25922) and S. epidermidis. This study represents the first report on chemical composition and biological activity of the Eucalyptus gunnii in the South Balkan region and beyond.
authors.affiliation: Department of Chemistry, Biochemistry and Environmental Protection, Faculty of Sciences, University of Novi Sad, Trg Dositeja Obradovica 3, Novi Sad 21000, Serbia. dbugarin@t-com.me.
authors.forename: Dušan
authors.initials: D
authors.lastname: Bugarin
authors.affiliation: Department of Chemistry, Biochemistry and Environmental Protection, Faculty of Sciences, University of Novi Sad, Trg Dositeja Obradovica 3, Novi Sad 21000, Serbia. grbovicslavenko@gmail.com.
authors.forename: Slavenko
authors.initials: S
authors.lastname: Grbović
authors.affiliation: Department of Chemistry, Biochemistry and Environmental Protection, Faculty of Sciences, University of Novi Sad, Trg Dositeja Obradovica 3, Novi Sad 21000, Serbia. orcicdejan@gmail.com.
authors.forename: Dejan
authors.initials: D
authors.lastname: Orčič
authors.affiliation: Chair of Microbiology, Faculty of Biology, University of Belgrade, Studentski Trg 3/II, Belgrade 11000, Serbia. mdragana@bio.bg.ac.rs.
authors.forename: Dragana
authors.initials: D
authors.lastname: Mitić-Ćulafić
authors.affiliation: Chair of Microbiology, Faculty of Biology, University of Belgrade, Studentski Trg 3/II, Belgrade 11000, Serbia.
authors.forename: Jelena
authors.initials: J
authors.lastname: Knežević-Vukčević
authors.affiliation: Department of Chemistry, Biochemistry and Environmental Protection, Faculty of Sciences, University of Novi Sad, Trg Dositeja Obradovica 3, Novi Sad 21000, Serbia. neda.mimica-dukic@dh.uns.ac.rs.
authors.forename: Neda
authors.initials: N
authors.lastname: Mimica-Dukić
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology*; D016587:Antimutagenic Agents / Q000737:chemistry / Q000494:pharmacology*; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects; D001713:Biphenyl Compounds / Q000737:chemistry; D005052:Eucalyptus / Q000737:chemistry*; D016166:Free Radical Scavengers / Q000737:chemistry / Q000494:pharmacology; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D008826:Microbial Sensitivity Tests; D009822:Oils, Volatile / Q000737:chemistry / Q000494:pharmacology*; D010851:Picrates / Q000737:chemistry; D018515:Plant Leaves / Q000737:chemistry
chemical_list: D000900:Anti-Bacterial Agents; D016587:Antimutagenic Agents; D000975:Antioxidants; D001713:Biphenyl Compounds; D016166:Free Radical Scavengers; D009822:Oils, Volatile; D010851:Picrates; C004931:1,1-diphenyl-2-picrylhydrazyl