title: Glyco-modification of protein withO-cyanate chain-end functionalized glycopolymerviaisourea bond formation.
abstract: Glycoengineering aimed at addition of carbohydrates to proteins is an attractive approach to alter pharmacokinetic properties of proteins such as enhancing stability and prolonging the duration of action. We report a novel protein glyco-modification of BSA and recombinant thrombomodulin withO-cyanate chain-end functionalized glycopolymerviaisourea bond formation. The protein glycoconjugates were confirmed by SDS-PAGE, western blot, and MALDI-TOF Mass Spectrometry. Protein C activation activity of the glyco-modified recombinant thrombomodulin was confirmed, proving no interference to activity from the glycopolymer modification. The isourea bond formation under mild conditions was demonstrated as an alternative method for protein modification with polymers.
authors.affiliation: Department of Chemistry, Chemical and Biomedical Engineering, and Center for Gene Regulation in Health and Disease (GRHD), Cleveland State University, 2121 Euclid Ave., Cleveland, OH 44115, USA.
authors.forename: Valentinas
authors.initials: V
authors.lastname: Gruzdys
authors.affiliation: Department of Chemistry, Chemical and Biomedical Engineering, and Center for Gene Regulation in Health and Disease (GRHD), Cleveland State University, 2121 Euclid Ave., Cleveland, OH 44115, USA.
authors.forename: Hailong
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Department of Chemistry, Chemical and Biomedical Engineering, and Center for Gene Regulation in Health and Disease (GRHD), Cleveland State University, 2121 Euclid Ave., Cleveland, OH 44115, USA.
authors.forename: Xue-Long
authors.initials: XL
authors.lastname: Sun
keywords: Carbohydrate; cyanate; glycopolymer; isourea; protein; thrombomodulin