title: The synthesis of a novel near-infrared fluorescent probe and its application in imaging of living cells.
abstract: IR-780, a novel near-infrared (NIR) fluorescent probe, was synthesized and applied to living cells. The probe exhibited good fluorescent characteristic, and cell experiments showed the probe had high affinity and without apparent cytotoxicity. Fluorescent image experiments in living A549 (Human lung adenocarcinoma epithelial cell line) and L929 (mouse fibroblast cell line) cells, further demonstrated its potential applications in biological systems. The probe effectively prevented the influence of autofluorescence and native cellular species in biological systems. It also exhibited excellent cell membrane permeability, good photostability, and high sensitivity.
authors.affiliation: School of Chemistry and Chemical Engineering, Southeast University, Nanjing, 210096, China, chunlongsun@163.com.
authors.forename: Chunlong
authors.initials: C
authors.lastname: Sun
authors.forename: Jin
authors.initials: J
authors.lastname: Cai
authors.forename: Junqing
authors.initials: J
authors.lastname: Chen
authors.forename: Yang
authors.initials: Y
authors.lastname: Wu
authors.forename: Peng
authors.initials: P
authors.lastname: Wang
authors.forename: Gaoxin
authors.initials: G
authors.lastname: Zhou
authors.forename: Xi
authors.initials: X
authors.lastname: Zong
authors.forename: Bo
authors.initials: B
authors.lastname: Chen
authors.forename: Yongfeng
authors.initials: Y
authors.lastname: Lv
authors.forename: Min
authors.initials: M
authors.lastname: Ji
mesh_terms: D000818:Animals; D002460:Cell Line; D002470:Cell Survival; D005456:Fluorescent Dyes / Q000138:chemical synthesis* / Q000737:chemistry; D006801:Humans; D007211:Indoles / Q000138:chemical synthesis* / Q000737:chemistry; D051379:Mice; D018613:Microscopy, Confocal; D057054:Molecular Imaging / Q000379:methods*; D066244:Proton Magnetic Resonance Spectroscopy; D013050:Spectrometry, Fluorescence; D019265:Spectroscopy, Near-Infrared
chemical_list: C548458:2-(2-(2-chloro-3-((1,3-dihydro-3,3-dimethyl-1-propyl-2H-indol-2-ylidene)ethylidene)-1-cyclohexen-1-yl)ethenyl)-3,3-dimethyl-1-propylindolium; D005456:Fluorescent Dyes; D007211:Indoles