title: Human papillomavirus prevalence in paired urine and cervical samples in women invited for cervical cancer screening.
abstract: With the introduction of Human papillomavirus (HPV) vaccination in young girls in 2007, it is important to monitor HPV infections and epidemiological changes in this target population. The present study has evaluated the detection of human papillomavirus DNA in paired cervical and urine samples to understand if HPV testing in urine could be used as non-invasive method to monitor HPV status in young women. The study enrolled 216 twenty five-year-old women, resident in Florence and invited for the first time to the cervical cancer Screening Program within a project evaluating the impact of HPV vaccination. HPV genotyping was performed on 216 paired urine and cervical samples. The overall concordance between cervix and urine samples, investigated by HPV genotyping (INNO-LiPA HPV Genotyping Extra), was: 85.6% (184/215), 84.6% (182/215), 80% (172/215) when the same HPV, at least the same HR HPV and all HR HPV, respectively, were detected. HPV type specific concordance in paired urine and cervical samples was observed in 85.8% (175/204) of women with normal cytology and in seven out of nine women with abnormal cytology. Urine seems to be a suitable and reliable biological material for HPV DNA detection as evidenced by the high concordance with HPV detected in cervical samples. These results suggest that urine could be a good noninvasive tool to monitor HPV infection in vaccinated women.
authors.affiliation: Unit of Analytical and Biomolecular Cytology, ISPO, Florence, Italy.
authors.forename: Elena
authors.initials: E
authors.lastname: Burroni
authors.forename: Paolo
authors.initials: P
authors.lastname: Bonanni
authors.forename: Cristina
authors.initials: C
authors.lastname: Sani
authors.forename: Vieri
authors.initials: V
authors.lastname: Lastrucci
authors.forename: Francesca
authors.initials: F
authors.lastname: Carozzi
authors.forename: Anna
authors.initials: A
authors.lastname: Iossa
authors.forename: Karin Louise
authors.initials: KL
authors.lastname: Andersson
authors.forename: Livia
authors.initials: L
authors.lastname: Brandigi
authors.forename: Carmelina
authors.initials: C
authors.lastname: Di Pierro
authors.forename: Massimo
authors.initials: M
authors.lastname: Confortini
authors.forename: Miriam
authors.initials: M
authors.lastname: Levi
authors.forename: Sara
authors.initials: S
authors.lastname: Boccalini
authors.forename: Laura
authors.initials: L
authors.lastname: Indiani
authors.forename: Antonino
authors.initials: A
authors.lastname: Sala
authors.forename: Tommaso
authors.initials: T
authors.lastname: Tanini
authors.forename: Angela
authors.initials: A
authors.lastname: Bechini
authors.forename: Chiara
authors.initials: C
authors.lastname: Azzari
mesh_terms: D000328:Adult; D002584:Cervix Uteri / Q000821:virology*; D005260:Female; D005838:Genotype; D060005:Genotyping Techniques / Q000379:methods; D006801:Humans; D007558:Italy / Q000453:epidemiology; D008403:Mass Screening / Q000379:methods; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D027383:Papillomaviridae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D030361:Papillomavirus Infections / Q000175:diagnosis* / Q000821:virology; D016032:Randomized Controlled Trials as Topic; D014556:Urine / Q000821:virology*; D002583:Uterine Cervical Neoplasms / Q000175:diagnosis*
keywords: HPV genotyping; cervical samples; human papillomavirus; urine