title: CRP acts as a transcriptional repressor of the YPO1635-phoPQ-YPO1632 operon in Yersinia pestis.
abstract: Yersinia pestis is the causative agent of plague. Both cyclic AMP receptor protein (CRP) and PhoP are involved in regulating virulence-related genes in Y. pestis. The phoPQ loci are transcribed as two distinct operons, YPO1635-phoPQ-YPO1632 and phoPQ-YPO1632. In the present work, the regulation of the first operon by CRP was investigated using primer extension, LacZ fusion, electrophoretic mobility shift assay, and DNase I footprinting assays. The results showed that CRP bound to a DNA region overlapping core promoter -10 element and transcription start of YPO1635 to repress the expression of YPO1635-phoPQ-YPO1632. Taken together with our previous results, complex regulatory interactions of CRP-AMP and PhoP/PhoQ were proposed in Y. pestis, which would contribute to tightly controlled expression of virulence-related genes.
authors.affiliation: School of Medicine, Jiangsu University, Zhenjiang, 212013, Jiangsu, China, zhangyiquanq@163.com.
authors.forename: Yiquan
authors.initials: Y
authors.lastname: Zhang
authors.forename: Fengjun
authors.initials: F
authors.lastname: Sun
authors.forename: Huiying
authors.initials: H
authors.lastname: Yang
authors.forename: Lei
authors.initials: L
authors.lastname: Liu
authors.forename: Bin
authors.initials: B
authors.lastname: Ni
authors.forename: Xinxiang
authors.initials: X
authors.lastname: Huang
authors.forename: Ruifu
authors.initials: R
authors.lastname: Yang
authors.forename: Dongsheng
authors.initials: D
authors.lastname: Zhou
mesh_terms: D001483:Base Sequence; D001665:Binding Sites; D002373:Cyclic AMP Receptor Protein / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D008969:Molecular Sequence Data; D009876:Operon; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D012333:RNA, Messenger / Q000235:genetics; D014158:Transcription, Genetic; D014774:Virulence / Q000235:genetics; D015010:Yersinia pestis / Q000235:genetics* / Q000378:metabolism* / Q000472:pathogenicity
chemical_list: D002373:Cyclic AMP Receptor Protein; D012333:RNA, Messenger