title: Process optimisation for the biosynthesis of cellulase by Bacillus PC-BC6 and its mutant derivative Bacillus N3 using submerged fermentation.
abstract: This study deals with optimisation of cultural conditions for enhanced production of cellulase by Bacillus PC-BC6 and its mutant derivative Bacillus N3. Influence of different variables including incubation time, temperature, inoculum size, pH, nitrogen sources and metal ions has been studied. The optimum conditions for cellulase production were incubation period of 72 h, inoculum size 4% incubation temperature 37°C, pH 7, 0.25% ammonium sulphate, 0.2% peptone as inorganic and organic nitrogen source in case of Bacillus PC-BC6. In case of mutant Bacillus N3, optimal conditions were incubation period of 48 h, incubation temperature 37°C, inoculum size 3%, pH 7, 0.2% ammonium chloride and 0.15% yeast extract. Presence of MnSO4 and CaCl2 enhances the enzyme production by Bacillus PC-BC6 and mutant Bacillus N3, respectively. This study was innovative and successful in producing cellulase economically by using cheap indigenous substrate Saccharum spontaneum.
authors.affiliation: a Department of Biotechnology , Lahore College for Women University , Lahore , Pakistan.
authors.forename: Roheena
authors.initials: R
authors.lastname: Abdullah
authors.forename: Wajeeha
authors.initials: W
authors.lastname: Zafar
authors.forename: Muhammad
authors.initials: M
authors.lastname: Nadeem
authors.forename: Mehwish
authors.initials: M
authors.lastname: Iqtedar
authors.forename: Shagufta
authors.initials: S
authors.lastname: Naz
authors.forename: Quratulain
authors.initials: Q
authors.lastname: Syed
authors.forename: Afshan
authors.initials: A
authors.lastname: Kaleem
mesh_terms: D001407:Bacillus / Q000378:metabolism*; D002480:Cellulase / Q000096:biosynthesis*; D003470:Culture Media / Q000737:chemistry; D005285:Fermentation; D006863:Hydrogen-Ion Concentration; D007218:Industrial Microbiology / Q000379:methods*; D031786:Saccharum / Q000737:chemistry; D013696:Temperature
chemical_list: D003470:Culture Media; D002480:Cellulase
keywords: Bacillus; cellulases; submerged fermentation