title: IMP-1 encoded by a novel Tn402-like class 1 integron in clinical Achromobacter xylosoxidans, China.
abstract: Achromobacter xylosoxidans strain A22732 is isolated from a pneumonia patient in China and produces carbapenemases OXA-114e and IMP-1, which are encoded by chromosome and plasmid, respectively, and confer resistance to multiple ß-lactam antibiotics including carbapenems. The blaIMP-1 gene together with aacA7 and orfE is captured by a novel Tn402-like class 1 integron in a conjugative IncP-1ß plasmid. In addition to the intrinsic integron promoter PcW, there is still a blaIMP-1 gene cassette-specific promoter. This is the first report of carbapenemase-encoding IncP-1ß plasmid in clinical bacterial isolate.
authors.affiliation: Nanlou Respiratory Diseases Department, Chinese People's Liberation Army General Hospital, Beijing 100853, China.
authors.forename: Zhenhong
authors.initials: Z
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, China.
authors.forename: Haihong
authors.initials: H
authors.lastname: Fang
authors.affiliation: Nanlou Respiratory Diseases Department, Chinese People's Liberation Army General Hospital, Beijing 100853, China.
authors.forename: Li
authors.initials: L
authors.lastname: Wang
authors.affiliation: Department of Pharmacy, Southwest Hospital, the Third Military Medical University, Chongqing 400038, China.
authors.forename: Fengjun
authors.initials: F
authors.lastname: Sun
authors.affiliation: Institute of Disease Control and Prevention, Beijing 100071, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, China.
authors.forename: Zhe
authors.initials: Z
authors.lastname: Yin
authors.affiliation: State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, China.
authors.forename: Huiying
authors.initials: H
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, China.
authors.forename: Wenhui
authors.initials: W
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Pharmacy, Southwest Hospital, the Third Military Medical University, Chongqing 400038, China.
authors.forename: Peiyuan
authors.initials: P
authors.lastname: Xia
authors.affiliation: State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, China.
authors.forename: Dongsheng
authors.initials: D
authors.lastname: Zhou
authors.affiliation: Nanlou Respiratory Diseases Department, Chinese People's Liberation Army General Hospital, Beijing 100853, China.
authors.forename: Changting
authors.initials: C
authors.lastname: Liu
mesh_terms: D042441:Achromobacter denitrificans / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics; D015780:Carbapenems / Q000378:metabolism; D002681:China; D002875:Chromosomes / Q000235:genetics; D004251:DNA Transposable Elements / Q000235:genetics*; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics; D032023:Integrons / Q000235:genetics*; D008826:Microbial Sensitivity Tests; D010957:Plasmids / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000235:genetics; D001618:beta-Lactamases / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; D015780:Carbapenems; D004251:DNA Transposable Elements; C409201:beta-lactamase IMP-1; D001618:beta-Lactamases; C063912:carbapenemase