title: Production of fructanase by a wild strain of Saccharomyces cerevisiae on tequila agave fructan.
abstract: A new wild strain of Saccharomyces cerevisiae (CF3) isolated from tequila must was evaluated for production of fructanase on Agave tequilana Weber fructan (FT). Fructanase activity (F) was assessed by a 3(3) factorial design (substrate, temperature and pH). High enzymatic activity (31.1 U/ml) was found at 30 °C, pH 5, using FT (10 g/l) as substrate. The effect of initial substrate concentration on F (FT0, 5.7-66 g/l) was studied and it was found that F was highest (44.8 U/ml) at FT0 25 g/l. A 2(2) factorial experimental design with five central points was utilized to study the effect of stirring and aeration on fructanase activity; stirring exhibited a stronger effect on F. The ratio fructanase to invertase (F/S) was 0.57, which confirms that the enzymes are fructanase. Crude fructanase reached high substrate hydrolysis (48 wt%) in 10 h. It is shown that S. cerevisiae CF3 was able to produce large amounts of fructanase by growing it on fructan from A. tequilana.
authors.affiliation: Biotechnology Processes Lab, Center of Exact Sciences and Engineering, Department of Chemical Engineering, University of Guadalajara, Blvd. Marcelino García Barragán # 1421, C. P. 44430, Guadalajara, Jalisco, Mexico.
authors.forename: R I
authors.initials: RI
authors.lastname: Corona-González
authors.forename: C
authors.initials: C
authors.lastname: Pelayo-Ortiz
authors.forename: G
authors.initials: G
authors.lastname: Jacques
authors.forename: G
authors.initials: G
authors.lastname: Guatemala
authors.forename: E
authors.initials: E
authors.lastname: Arriola
authors.forename: J A
authors.initials: JA
authors.lastname: Arias
authors.forename: G
authors.initials: G
authors.lastname: Toriz
mesh_terms: D027882:Agave / Q000737:chemistry* / Q000382:microbiology; D005630:Fructans / Q000378:metabolism*; D006026:Glycoside Hydrolases / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000254:growth & development / Q000378:metabolism*; D013696:Temperature; D043324:beta-Fructofuranosidase / Q000378:metabolism
chemical_list: D005630:Fructans; D006026:Glycoside Hydrolases; D043324:beta-Fructofuranosidase; C021512:levanase