title: Homing and restorative effects of bone marrow-derived mesenchymal stem cells on cisplatin injured ovaries in rats.
abstract: Premature ovarian failure (POF) is a long-term adverse effect of chemotherapy treatment. However, current available treatment regimens are not optimal. Emerging evidence suggests that bone marrow-derived mesenchymal stem cells (BMSCs) could restore the structure and function of injured tissues, but the homing and restorative effects of BMSCs on chemotherapy injured ovaries are still not clear. In this study, we found that granulosa cell (GC) apoptosis induced by cisplatin was reduced when BMSCs were migrated to granulosa cells (GCs) in vitro. Chemotherapy-induced POF was induced by intraperitoneal injection of cisplatin in rats. BMSCs labeled with enhanced green fluorescent protein (EGFP) were injected into the rats via the tail vein to investigate the homing and distribution of BMSCs in vivo. The number of BMSCs in the ovarian hilum and medulla was greater than in the cortex, but no BMSCs were found in the follicles and corpus lutea. In addition, the BMSCs treatment group's antral follicle count and estradiol levels increased after 30 days, compared with the POF group. Hence, our study demonstrates that intravenously delivered BMSCs can home to the ovaries, and restore its structure and function in POF model rats.
authors.affiliation: Liaoning Medical University, Jinzhou, Liaoning 121001, China ; Department of Gynecology and Obstetrics, Third Affiliated Hospital of Liaoning Medical University, Jinzhou, Liaoning 121001, China ; Key laboratory of Follicular Development and Reproductive Health of Liaoning Province, Liaoning Medical University, Jinzhou, Liaoning 121001, China.
authors.forename: Jiabin
authors.initials: J
authors.lastname: Liu
authors.affiliation: Liaoning Medical University, Jinzhou, Liaoning 121001, China.
authors.forename: Haiying
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Department of Gynecology and Obstetrics, Third Affiliated Hospital of Liaoning Medical University, Jinzhou, Liaoning 121001, China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Gynecology and Obstetrics, Third Affiliated Hospital of Liaoning Medical University, Jinzhou, Liaoning 121001, China.
authors.forename: Nan
authors.initials: N
authors.lastname: Li
authors.affiliation: Department of Gynecology and Obstetrics, Third Affiliated Hospital of Liaoning Medical University, Jinzhou, Liaoning 121001, China.
authors.forename: Yuku
authors.initials: Y
authors.lastname: Wen
authors.affiliation: Department of Gynecology and Obstetrics, Third Affiliated Hospital of Liaoning Medical University, Jinzhou, Liaoning 121001, China.
authors.forename: Fanglei
authors.initials: F
authors.lastname: Cao
authors.affiliation: Liaoning Medical University, Jinzhou, Liaoning 121001, China ; Department of Gynecology and Obstetrics, Third Affiliated Hospital of Liaoning Medical University, Jinzhou, Liaoning 121001, China ; Key laboratory of Follicular Development and Reproductive Health of Liaoning Province, Liaoning Medical University, Jinzhou, Liaoning 121001, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Ai
authors.affiliation: Dongzhimen Hospital Affiliated to Beijing University of China Medicine, Beijing 100029, China.
authors.forename: Xiaoou
authors.initials: X
authors.lastname: Xue
mesh_terms: D000818:Animals; D017209:Apoptosis; D016026:Bone Marrow Transplantation / Q000379:methods; D002465:Cell Movement; D002478:Cells, Cultured; D002945:Cisplatin / Q000008:administration & dosage* / Q000009:adverse effects; D005260:Female; D006107:Granulosa Cells / Q000378:metabolism / Q000473:pathology; D006801:Humans; D007274:Injections, Intraperitoneal; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods*; D059630:Mesenchymal Stem Cells / Q000502:physiology*; D009928:Organ Specificity; D016649:Primary Ovarian Insufficiency / Q000139:chemically induced* / Q000628:therapy*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D002945:Cisplatin
keywords: bone marrow-derived mesenchymal stem cells; cisplatin; homing; intravenous injection; premature ovarian failure