title: Cellular inorganic carbon fluxes in Trichodesmium: a combined approach using measurements and modelling.
abstract: To predict effects of climate change on phytoplankton, it is crucial to understand how their mechanisms for carbon acquisition respond to environmental conditions. Aiming to shed light on the responses of extra- and intracellular inorganic C (Ci) fluxes, the cyanobacterium Trichodesmium erythraeum IMS101 was grown with different nitrogen sources (N2 vs NO3 (-)) and pCO2 levels (380 vs 1400 µatm). Cellular Ci fluxes were assessed by combining membrane inlet mass spectrometry (MIMS), (13)C fractionation measurements, and modelling. Aside from a significant decrease in Ci affinity at elevated pCO2 and changes in CO2 efflux with different N sources, extracellular Ci fluxes estimated by MIMS were largely unaffected by the treatments. (13)C fractionation during biomass production, however, increased with pCO2, irrespective of the N source. Strong discrepancies were observed in CO2 leakage estimates obtained by MIMS and a (13)C-based approach, which further increased under elevated pCO2. These offsets could be explained by applying a model that comprises extracellular CO2 and HCO3 (-) fluxes as well as internal Ci cycling around the carboxysome via the CO2 uptake facilitator NDH-14. Assuming unidirectional, kinetic fractionation between CO2 and HCO3 (-) in the cytosol or enzymatic fractionation by NDH-14, both significantly improved the comparability of leakage estimates. Our results highlight the importance of internal Ci cycling for (13)C composition as well as cellular energy budgets of Trichodesmium, which ought to be considered in process studies on climate change effects.
authors.affiliation: Marine Biogeosciences, Alfred Wegener Institute, Helmholtz Centre for Polar and Marine Research, Am Handelshafen 12, 27570 Bremerhaven, Germany meri.eichner@awi.de.
authors.forename: Meri
authors.initials: M
authors.lastname: Eichner
authors.affiliation: Marine Biogeosciences, Alfred Wegener Institute, Helmholtz Centre for Polar and Marine Research, Am Handelshafen 12, 27570 Bremerhaven, Germany.
authors.forename: Silke
authors.initials: S
authors.lastname: Thoms
authors.affiliation: Department for Geosciences, Princeton University, Princeton, NJ 08540, USA Present address: Department of Earth, Ocean and Atmospheric Sciences, Florida State University, Tallahassee, Fl 32306, USA.
authors.forename: Sven A
authors.initials: SA
authors.lastname: Kranz
authors.affiliation: Marine Biogeosciences, Alfred Wegener Institute, Helmholtz Centre for Polar and Marine Research, Am Handelshafen 12, 27570 Bremerhaven, Germany.
authors.forename: Björn
authors.initials: B
authors.lastname: Rost
mesh_terms: D057486:Carbon Cycle; D002245:Carbon Dioxide / Q000378:metabolism*; D005591:Chemical Fractionation; D000458:Cyanobacteria / Q000378:metabolism*; D013058:Mass Spectrometry; D008954:Models, Biological; D009566:Nitrates / Q000378:metabolism*; D009584:Nitrogen / Q000378:metabolism*; D010839:Phytoplankton / Q000378:metabolism
chemical_list: D009566:Nitrates; D002245:Carbon Dioxide; D009584:Nitrogen
keywords: CO2; Carbon acquisition; NDH; carbon-concentrating mechanism (CCM); cyanobacteria; leakage; ocean acidification.