title: Methanolic soluble fractions of lingzhi or reishi medicinal mushroom, Ganoderma lucidum (higher Basidiomycetes) extract inhibit neuraminidase activity in Newcastle disease virus (LaSota).
abstract: The antineuraminidase activity of different organic soluble fractions of Ganoderma lucidum extract was investigated using inhibition of hemagglutination and elution of chicken erythrocytes by Newcastle disease virus (NDV). Fractions of methanol, ethylacetate, and normal butanol (n-butanol) of the G. lucidum were tested against neuraminidase producing NDV as antigen. Different dilutions of the organic soluble fractions inhibited elution of 1% red blood cells by neuraminidase of NDV While the methanolic and n-butanol extracts inhibited neuraminidase activity even at a dilution of 1:16 and that of ethylacetate fraction inhibited even at 1:32 respectively. This finding indicates that G. lucidum has some antineuraminidase activity against NDV and may be exploited in the management of NDV infection.
authors.affiliation: Departments of Veterinary Physiology, Pharmacology, and Biochemistry, Faculty of Veterinary Medicine, University of Maiduguri, Maiduguri, Nigeria.
authors.forename: Bala U
authors.initials: BU
authors.lastname: Shamaki
authors.affiliation: Departments of Veterinary Physiology, Pharmacology, and Biochemistry, Faculty of Veterinary Medicine, University of Maiduguri, Maiduguri, Nigeria.
authors.forename: Umar K
authors.initials: UK
authors.lastname: Sandabe
authors.affiliation: Department of Animal Science, Faculty of Agriculture, Nassarawa State University, Lafia, Nigeria.
authors.forename: Adamu O
authors.initials: AO
authors.lastname: Ogbe
authors.affiliation: Department of Chemistry, Faculty of Science, University of Maiduguri, Maiduguri, Nigeria.
authors.forename: Fanna I
authors.initials: FI
authors.lastname: Abdulrahman
authors.affiliation: Animal Virus Research Laboratory, Department of Veterinary Microbiology and Parasitology, Faculty of Veterinary Medicine, University of Maiduguri, Maiduguri, Nigeria.
authors.forename: Abdul-Dahiru
authors.initials: AD
authors.lastname: El-Yuguda
mesh_terms: D000998:Antiviral Agents / Q000737:chemistry / Q000494:pharmacology; D032902:Ganoderma / Q000737:chemistry*; D000432:Methanol / Q000737:chemistry*; D009439:Neuraminidase / Q000037:antagonists & inhibitors* / Q000378:metabolism*; D009522:Newcastle disease virus / Q000378:metabolism*
chemical_list: D000998:Antiviral Agents; D009439:Neuraminidase; D000432:Methanol