title: Taking advantage of reduced droplet-surface interaction to optimize transport of bioanalytes in digital microfluidics.
abstract: Digital microfluidics (DMF), a technique for manipulation of droplets, is a promising alternative for the development of "lab-on-a-chip" platforms. Often, droplet motion relies on the wetting of a surface, directly associated with the application of an electric field; surface interactions, however, make motion dependent on droplet contents, limiting the breadth of applications of the technique. Some alternatives have been presented to minimize this dependence. However, they rely on the addition of extra chemical species to the droplet or its surroundings, which could potentially interact with droplet moieties. Addressing this challenge, our group recently developed Field-DW devices to allow the transport of cells and proteins in DMF, without extra additives. Here, the protocol for device fabrication and operation is provided, including the electronic interface for motion control. We also continue the studies with the devices, showing that multicellular, relatively large, model organisms can also be transported, arguably unaffected by the electric fields required for device operation.
authors.affiliation: Department of Mathematics, Physics and Statistics, University of the Sciences; s.freire@usciences.edu.
authors.forename: Sergio L S
authors.initials: SL
authors.lastname: Freire
authors.affiliation: Department of Mathematics, Physics and Statistics, University of the Sciences.
authors.forename: Nathaniel
authors.initials: N
authors.lastname: Thorne
authors.affiliation: Department of Mathematics, Physics and Statistics, University of the Sciences.
authors.forename: Michael
authors.initials: M
authors.lastname: Wutkowski
authors.affiliation: Department of Mathematics, Physics and Statistics, University of the Sciences.
authors.forename: Selina
authors.initials: S
authors.lastname: Dao
mesh_terms: D000818:Animals; D017173:Caenorhabditis elegans; D004581:Electronics; D004867:Equipment Design; D056656:Lab-On-A-Chip Devices; D044085:Microfluidics / Q000295:instrumentation* / Q000379:methods; D011506:Proteins / Q000737:chemistry; D013499:Surface Properties; D017155:Wettability
chemical_list: D011506:Proteins