title: Single primer-triggered isothermal amplification for double-stranded DNA detection.
abstract: Here we have devised a new generation of isothermal double-stranded DNA (dsDNA) detection method, termed single primer-triggered isothermal amplification (SAMP). It is very simple only requiring one primer and a few copies of dsDNA in less than an hour are detectable with multiple signal amplification steps.
authors.affiliation: Shandong Provincial Key Laboratory of Biochemical Analysis, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, P. R. China. sc169@163.com.
authors.forename: Cuiping
authors.initials: C
authors.lastname: Ma
authors.forename: Dianang
authors.initials: D
authors.lastname: Han
authors.forename: Meilian
authors.initials: M
authors.lastname: Deng
authors.forename: Jingfei
authors.initials: J
authors.lastname: Wang
authors.forename: Chao
authors.initials: C
authors.lastname: Shi
mesh_terms: D004247:DNA / Q000032:analysis*; D017931:DNA Primers; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D013997:Time Factors
chemical_list: D017931:DNA Primers; D004247:DNA