title: Advancing small-molecule-based chemical biology with next-generation sequencing technologies.
abstract: Next-generation-sequencing (NGS) technologies enable us to obtain extensive information by deciphering millions of individual DNA sequencing reactions simultaneously. The new DNA-sequencing strategies exceed their precursors in output by many orders of magnitude, resulting in a quantitative increase in valuable sequence information that could be harnessed for qualitative analysis. Sequencing on this scale has facilitated significant advances in diverse disciplines, ranging from the discovery, design, and evaluation of many small molecules and relevant biological mechanisms to maturation of personalized therapies. NGS technologies that have recently become affordable allow us to gain in-depth insight into small-molecule-triggered biological phenomena and empower researchers to develop advanced versions of small molecules. In this review we focus on the overlooked implications of NGS technologies in chemical biology, with a special emphasis on small-molecule development and screening.
authors.affiliation: Department of Chemistry, Graduate School of Science, Kyoto University, Sakyo-ku, Kyoto 606-8502 (Japan).
authors.forename: Chandran
authors.initials: C
authors.lastname: Anandhakumar
authors.forename: Seiichiro
authors.initials: S
authors.lastname: Kizaki
authors.forename: Toshikazu
authors.initials: T
authors.lastname: Bando
authors.forename: Ganesh N
authors.initials: GN
authors.lastname: Pandian
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Sugiyama
mesh_terms: D001671:Biochemistry; D019295:Computational Biology; D055808:Drug Discovery; D015894:Genome, Human; D059014:High-Throughput Nucleotide Sequencing / Q000295:instrumentation / Q000379:methods*; D057166:High-Throughput Screening Assays; D006801:Humans; D009690:Nucleic Acid Conformation; D057285:Precision Medicine; D054852:Small Molecule Libraries / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D054852:Small Molecule Libraries
keywords: aptamers; chemical biology; genomics; next-generation sequencing; small molecules