title: Synergistic template-free synthesis of dsDNA by Thermococcus nautili primase PolpTN2, DNA polymerase PolB, and pTN2 helicase.
abstract: A combination of three enzymes from the hyperthermophilic archaeon Thermococcus nautili, DNA primase PolpTN2, DNA polymerase PolB, and pTN2 DNA helicase, was found to synthesize up to 300-400 ng/µl dsDNA from deoxynucleotide triphosphates in less than 30 min in the absence of added template DNA and oligonucleotide primer. The reaction did not occur below 64 °C. No synthesis was observed if PolpTN2 or PolB were left out; helicase was not essential but accelerated the reaction. The DNA synthesized consisted of highly reiterated palindromic sequences reaching up to more that 10 kb. Sequence analysis of three independent reaction products synthesized at different temperatures showed that the palindromes shared a common pentanucleotide core, suggesting that random nucleic acid fragments were not responsible for priming the reaction. When enzymes were added sequentially, preincubation with primase plus helicase followed by PolB led to a shorter delay before the onset of the reaction as compared to preincubation with PolB plus helicase followed by primase. This suggests that the primase generates seeds that are subsequently amplified and elongated in synergy with PolB by a mechanism involving hairpin formation and slippage synthesis.
authors.affiliation: Unité Biologie moléculaire du Gène chez les Extrêmophiles, Département de Microbiologie, Institut Pasteur, 25-28, rue du Dr Roux, 75724, Paris Cedex 15, France, pierre.beguin@pasteur.fr.
authors.forename: Pierre
authors.initials: P
authors.lastname: Béguin
authors.forename: Sukhvinder
authors.initials: S
authors.lastname: Gill
authors.forename: Nicole
authors.initials: N
authors.lastname: Charpin
authors.forename: Patrick
authors.initials: P
authors.lastname: Forterre
mesh_terms: D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000096:biosynthesis*; D004265:DNA Helicases / Q000235:genetics*; D004257:DNA Polymerase II / Q000235:genetics*; D019915:DNA Primase / Q000235:genetics*; D017931:DNA Primers / Q000235:genetics; D004587:Electrophoresis, Agar Gel; D008836:Micrococcal Nuclease / Q000737:chemistry; D008969:Molecular Sequence Data; D009696:Nucleic Acids / Q000737:chemistry; D013696:Temperature; D019712:Thermococcus / Q000201:enzymology*
chemical_list: D017931:DNA Primers; D009696:Nucleic Acids; D004247:DNA; D019915:DNA Primase; D004257:DNA Polymerase II; D008836:Micrococcal Nuclease; D004265:DNA Helicases