title: Two bacterial group II phosphopantetheinyl transferases involved in both primary metabolism and secondary metabolism.
abstract: It is known that bacterial group II phosphopantetheinyl transferases (PPTases) usually phosphopantetheinylate acyl carrier proteins (ACPs) involved in the secondary metabolism. For example, a bacterial group II PPTase SchPPT has been known to phosphopantetheinylate only ACPs involved in secondary metabolism, such as scn ACP0-2 and scn ACP7. In this study, we found two bacterial group II PPTases, Hppt and Sppt, could phosphopantetheinylate not only scn ACP0-2 and scn ACP7, but also sch FAS ACP, an ACP involved in primary metabolism. Swapping of the N terminus and C terminus of PPTases showed that (i) both the hybrids Hppt-Sppt and Sppt-Hppt could phosphopantetheinylate sch FAS ACP but not scn ACP0-2; (ii) both the hybrids Sppt-SchPPT and SchPPT-Sppt lost abilities to phosphopantetheinylate sch FAS ACP and scn ACP0-2. Hppt and Sppt represent group II PPTases which phosphopantetheinylate both ACPs involved in primary metabolism and ACPs involved in secondary metabolism.
authors.affiliation: College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang, China.
authors.forename: Yue-Yue
authors.initials: YY
authors.lastname: Wang
authors.forename: Xiao-Sheng
authors.initials: XS
authors.lastname: Zhang
authors.forename: Ni-Ni
authors.initials: NN
authors.lastname: Ren
authors.forename: Yuan-Yang
authors.initials: YY
authors.lastname: Guo
authors.forename: Xin-Hang
authors.initials: XH
authors.lastname: Jiang
authors.forename: Hui
authors.initials: H
authors.lastname: Jiang
authors.forename: Yu-Dong
authors.initials: YD
authors.lastname: Li
authors.forename: Yong-Quan
authors.initials: YQ
authors.lastname: Li
mesh_terms: D000595:Amino Acid Sequence; D001419:Bacteria / Q000235:genetics / Q000378:metabolism*; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D001481:Basal Metabolism; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism; D002384:Catalysis; D004789:Enzyme Activation; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D010802:Phylogeny; D054730:Protein Interaction Domains and Motifs; D064210:Secondary Metabolism; D017855:Transferases (Other Substituted Phosphate Groups) / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D002352:Carrier Proteins; C082662:phosphopantetheinyl transferase; D017855:Transferases (Other Substituted Phosphate Groups)