title: High-density lipoprotein profile in newly-diagnosed lower extremity artery disease in Slovak population without diabetes mellitus.
abstract: OBJECTIVE
  To assess the relationship of high density lipoprotein subfractions to newly-diagnosed lower extremity artery disease (LEAD) in individuals without diabetes mellitus and without hypolipidemic therapy.
  
  
  METHODS
  This cross-sectional study involves 106 subjects: 51 had newly diagnosed LEAD and no diabetes anamnesis and were not on hypolipidemic therapy; and 55 controls were without clinical presentation of LEAD and were normolipidemic. Analysis of HDL subclasses was performed by an innovative electrophoresis method on polyacrylamide gel (PAG), the Lipoprint HDL System.
  
  
  RESULTS
  In LEAD subjects, total HDL-C levels as well as HDL2 (intermediate-to-large particles) subfraction levels were decreased (p<0.0001 and p<0.019 respectively). Interestingly the HDL3 (small particles) subfraction was significantly higher and lost its proportional relationship within the HDL cholesterol fraction (p<0.025, p<0.01 respectively).
  
  
  CONCLUSIONS
  These findings pointed out that: (i) the reduction of HDL-C and especially HDL2 subpopulation opposite to the increase of small HDL3 subclass may be considered as important predictors of cardiovascular diseases. (ii) there are undisputable advantages of using Lipoprint HDL to identify HDL subfractions; the presence of high concentration of small HDL in patients with PAD/LEAD emphasizes that the potentially proatherogenic subclass of HDL family is linked to small HDL.
authors.affiliation: 2nd Department of Internal Medicine, Faculty of Medicine, Comenius University, Bratislava, Slovakia.
authors.forename: Martin
authors.initials: M
authors.lastname: Kasko
authors.affiliation: 2nd Department of Internal Medicine, Faculty of Medicine, Comenius University, Bratislava, Slovakia.
authors.forename: Ludovit
authors.initials: L
authors.lastname: Gaspar
authors.affiliation: 2nd Department of Internal Medicine, Faculty of Medicine, Comenius University, Bratislava, Slovakia.
authors.forename: Andrej
authors.initials: A
authors.lastname: Dukát
authors.affiliation: 2nd Department of Internal Medicine, Faculty of Medicine, Comenius University, Bratislava, Slovakia.
authors.forename: Peter
authors.initials: P
authors.lastname: Gavorník
authors.affiliation: 2nd Department of Internal Medicine, Faculty of Medicine, Comenius University, Bratislava, Slovakia.
authors.forename: Stanislav
authors.initials: S
authors.lastname: Oravec
mesh_terms: D000368:Aged; D050197:Atherosclerosis / Q000378:metabolism; D003430:Cross-Sectional Studies; D003920:Diabetes Mellitus; D004586:Electrophoresis / Q000379:methods*; D005260:Female; D006801:Humans; D053439:Lipoproteins, HDL2 / Q000097:blood* / Q000737:chemistry; D053440:Lipoproteins, HDL3 / Q000097:blood* / Q000737:chemistry; D008297:Male; D008875:Middle Aged; D058729:Peripheral Arterial Disease / Q000378:metabolism*; D011237:Predictive Value of Tests; D018154:Slovakia
chemical_list: D053439:Lipoproteins, HDL2; D053440:Lipoproteins, HDL3