title: Chromosomal location and comparative genomics analysis of powdery mildew resistance gene Pm51 in a putative wheat-Thinopyrum ponticum introgression line.
abstract: Powdery mildew (PM) is a very destructive disease of wheat (Triticum aestivum L.). Wheat-Thinopyrum ponticum introgression line CH7086 was shown to possess powdery mildew resistance possibly originating from Th. ponticum. Genomic in situ hybridization and molecular characterization of the alien introgression failed to identify alien chromatin. To study the genetics of resistance, CH7086 was crossed with susceptible genotypes. Segregation in F2 populations and F2:3 lines tested with Chinese Bgt race E09 under controlled conditions indicated that CH7086 carries a single dominant gene for powdery mildew resistance. Fourteen SSR and EST-PCR markers linked with the locus were identified. The genetic distances between the locus and the two flanking markers were 1.5 and 3.2 cM, respectively. Based on the locations of the markers by nullisomic-tetrasomic and deletion lines of 'Chinese Spring', the resistance gene was located in deletion bin 2BL-0.89-1.00. Conserved orthologous marker analysis indicated that the genomic region flanking the resistance gene has a high level of collinearity to that of rice chromosome 4 and Brachypodium chromosome 5. Both resistance specificities and tests of allelism suggested the resistance gene in CH7086 was different from previously reported powdery mildew resistance genes on 2BL, and the gene was provisionally designated PmCH86. Molecular analysis of PmCH86 compared with other genes for resistance to Bgt in the 2BL-0.89-1.00 region suggested that PmCH86 may be a new PM resistance gene, and it was therefore designated as Pm51. The closely linked flanking markers could be useful in exploiting this putative wheat-Thinopyrum translocation line for rapid transfer of Pm51 to wheat breeding programs.
authors.affiliation: School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, Sichuan, China; Crop Science Institute, Shanxi Academy of Agricultural Sciences, Taiyuan, Shanxi, China.
authors.forename: Haixian
authors.initials: H
authors.lastname: Zhan
authors.affiliation: School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, Sichuan, China.
authors.forename: Guangrong
authors.initials: G
authors.lastname: Li
authors.affiliation: Crop Science Institute, Shanxi Academy of Agricultural Sciences, Taiyuan, Shanxi, China.
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Crop Science Institute, Shanxi Academy of Agricultural Sciences, Taiyuan, Shanxi, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Li
authors.affiliation: Crop Science Institute, Shanxi Academy of Agricultural Sciences, Taiyuan, Shanxi, China.
authors.forename: Huijuan
authors.initials: H
authors.lastname: Guo
authors.affiliation: School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, Sichuan, China.
authors.forename: Wenping
authors.initials: W
authors.lastname: Gong
authors.affiliation: College of agronomy, Shanxi Agricultural University, Taigu, Shanxi, China.
authors.forename: Juqing
authors.initials: J
authors.lastname: Jia
authors.affiliation: Crop Science Institute, Shanxi Academy of Agricultural Sciences, Taiyuan, Shanxi, China.
authors.forename: Linyi
authors.initials: L
authors.lastname: Qiao
authors.affiliation: Crop Science Institute, Shanxi Academy of Agricultural Sciences, Taiyuan, Shanxi, China.
authors.forename: Yongkang
authors.initials: Y
authors.lastname: Ren
authors.affiliation: School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, Sichuan, China.
authors.forename: Zujun
authors.initials: Z
authors.lastname: Yang
authors.affiliation: Crop Science Institute, Shanxi Academy of Agricultural Sciences, Taiyuan, Shanxi, China; Key Lab of Crop Gene Resources and Germplasm Enhancement on Loess Plateau, Ministry of Agriculture, Taiyuan, Shanxi, China.
authors.forename: Zhijian
authors.initials: Z
authors.lastname: Chang
mesh_terms: D001947:Breeding; D002874:Chromosome Mapping / Q000379:methods*; D032461:Chromosomes, Plant / Q000235:genetics; D055028:Comparative Genomic Hybridization / Q000379:methods*; D003433:Crosses, Genetic; D060467:Disease Resistance; D020224:Expressed Sequence Tags; D018895:Microsatellite Repeats; D010935:Plant Diseases / Q000235:genetics / Q000382:microbiology; D010940:Plant Proteins / Q000235:genetics*; D014908:Triticum / Q000235:genetics* / Q000382:microbiology
chemical_list: D010940:Plant Proteins