title: A simple bioluminescent method for measuring D-amino acid oxidase activity.
abstract: D-Amino acid oxidase (DAO) plays important roles in regulating D-amino acid neurotransmitters and was recently identified as a key enzyme integral to hydrogen sulfide production from D-Cys. We report here the development of a simple biocompatible, bioluminescent method for measuring DAO activity based on the highly selective condensation of D-Cys with 6-hydroxy-2-cyanobenzothiazole (CBT-OH) to form D-luciferin.
authors.affiliation: Department of Chemistry and Biochemistry, Institute of Molecular Biology, Materials Science Institute, University of Oregon, Eugene, OR 97403, USA. pluth@uoregon.edu.
authors.forename: T Spencer
authors.initials: TS
authors.lastname: Bailey
authors.forename: Micah T
authors.initials: MT
authors.lastname: Donor
authors.forename: Sean P
authors.initials: SP
authors.lastname: Naughton
authors.forename: Michael D
authors.initials: MD
authors.lastname: Pluth
mesh_terms: D052160:Benzothiazoles / Q000737:chemistry / Q000378:metabolism*; D003605:D-Amino-Acid Oxidase / Q000737:chemistry / Q000378:metabolism*; D004789:Enzyme Activation; D008163:Luminescent Measurements; D008958:Models, Molecular; D015394:Molecular Structure
chemical_list: D052160:Benzothiazoles; C532924:D-luciferin; D003605:D-Amino-Acid Oxidase