title: Elucidation of the interaction mechanism with liposomes of gH625-peptide functionalized dendrimers.
abstract: We have demonstrated that amide-based dendrimers functionalized with the membrane-interacting peptide gH625 derived from the herpes simplex virus type 1 (HSV-1) envelope glycoprotein H enter cells mainly through a non-active translocation mechanism. Herein, we investigate the interaction between the peptide-functionalized dendrimer and liposomes composed of PC/Chol using fluorescence spectroscopy, isothermal titration calorimetry, and surface plasmon resonance to get insights into the mechanism of internalization. The affinity for the membrane bilayer is very high and the interaction between the peptide-dendrimer and liposomes took place without evidence of pore formation. These results suggest that the presented peptidodendrimeric scaffold may be a promising material for efficient drug delivery.
authors.affiliation: Department of Pharmacy & CIRPEB & DFM Scarl, University of Naples "Federico II", Naples, Italy.
authors.forename: Annarita
authors.initials: A
authors.lastname: Falanga
authors.affiliation: Molecular Design Institute and Department of Chemistry, New York University, New York, New York, United States of America.
authors.forename: Rossella
authors.initials: R
authors.lastname: Tarallo
authors.affiliation: Molecular Design Institute and Department of Chemistry, New York University, New York, New York, United States of America.
authors.forename: Thomas
authors.initials: T
authors.lastname: Carberry
authors.affiliation: Department of Experimental Medicine, Second University of Naples, Naples, Italy.
authors.forename: Massimiliano
authors.initials: M
authors.lastname: Galdiero
authors.affiliation: Molecular Design Institute and Department of Chemistry, New York University, New York, New York, United States of America.
authors.forename: Marcus
authors.initials: M
authors.lastname: Weck
authors.affiliation: Department of Pharmacy & CIRPEB & DFM Scarl, University of Naples "Federico II", Naples, Italy.
authors.forename: Stefania
authors.initials: S
authors.lastname: Galdiero
mesh_terms: D050091:Dendrimers / Q000737:chemistry*; D008081:Liposomes / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry* / Q000378:metabolism; D011485:Protein Binding; D014759:Viral Envelope Proteins / Q000737:chemistry* / Q000378:metabolism
chemical_list: D050091:Dendrimers; D008081:Liposomes; D010455:Peptides; D014759:Viral Envelope Proteins; C569201:gH625 peptide, Herpes simplex virus type I