title: High-throughput cell death assays.
abstract: High-throughput screens (HTS ) are powerful tools that permit the rapid evaluation of thousands of samples in a cost-effective manner and minimize sample and reagent consumption. Such assays have recently begun to be utilized to evaluate cell death modalities and also the cytoprotective efficacy of compounds against a wide variety of stresses. Here we describe the design, preparation, and undertaking of HTS-appropriate assays that utilize simple and cost-effective fluorophore - and luminescence -based functional readouts of cell viability. These assays permit the examination of 96–384 compounds in a single multiwell plate with highly robust statistical significance at a fraction of the financial and work cost of traditional approaches.
authors.affiliation: Department of Zoology, University of British Columbia, #4200-6270 University Blvd, Vancouver, BC, V6T 1Z4, Canada, pamenter@zoology.ubc.ca.
authors.forename: Matthew E
authors.initials: ME
authors.lastname: Pamenter
authors.forename: Gabriel G
authors.initials: GG
authors.lastname: Haddad
mesh_terms: D016923:Cell Death / Q000187:drug effects / Q000235:genetics; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D004353:Drug Evaluation, Preclinical; D057166:High-Throughput Screening Assays / Q000379:methods*; D006801:Humans; D008967:Molecular Biology / Q000379:methods