title: Immunomodulatory Potential of Human Adipose Mesenchymal Stem Cells Derived Exosomes on in vitro Stimulated T Cells.
abstract: In the recent years, it has been demonstrated that the biological activity of mesenchymal stem cells (MSCs) is mediated through the release of paracrine factors. Many of these factors are released into exosomes, which are small membranous vesicles that participate in cell-cell communication. Exosomes from MSCs are thought to have similar functions to MSCs such as repairing and regeneration of damaged tissue, but little is known about the immunomodulatory effect of these vesicles. Based on an extensive bibliography where the immunomodulatory capacity of MSCs has been demonstrated, here we hypothesized that released exosomes from MSCs may have an immunomodulatory role on the differentiation, activation and function of different lymphocyte subsets. According to this hypothesis, in vitro experiments were performed to characterize the immunomodulatory effect of human adipose MSCs derived exosomes (exo-hASCs) on in vitro stimulated T cells. The phenotypic characterization of cytotoxic and helper T cells (activation and differentiation markers) together with functional assays (proliferation and IFN-γ production) demonstrated that exo-hASCs exerted an inhibitory effect in the differentiation and activation of T cells as well as a reduced T cell proliferation and IFN-γ release on in vitro stimulated cells. In summary, here we demonstrate that MSCs-derived exosomes are a cell-derived product that could be considered as a therapeutic agent for the treatment of inflammation-related diseases.
authors.affiliation: Stem Cell Therapy Unit, Minimally Invasive Surgery Centre Jesus Uson , Cáceres , Spain.
authors.forename: Rebeca
authors.initials: R
authors.lastname: Blazquez
authors.affiliation: Stem Cell Therapy Unit, Minimally Invasive Surgery Centre Jesus Uson , Cáceres , Spain.
authors.forename: Francisco Miguel
authors.initials: FM
authors.lastname: Sanchez-Margallo
authors.affiliation: Research and Development Department, TiGenix SA, Parque Tecnológico de Madrid , Madrid , Spain.
authors.forename: Olga
authors.initials: O
authors.lastname: de la Rosa
authors.affiliation: Technical Operations, TiGenix NV , Leuven , Belgium.
authors.forename: Wilfried
authors.initials: W
authors.lastname: Dalemans
authors.affiliation: Stem Cell Therapy Unit, Minimally Invasive Surgery Centre Jesus Uson , Cáceres , Spain.
authors.forename: Verónica
authors.initials: V
authors.lastname: Alvarez
authors.affiliation: Immunology Unit, Department of Physiology, University of Extremadura , Cáceres , Spain.
authors.forename: Raquel
authors.initials: R
authors.lastname: Tarazona
authors.affiliation: Stem Cell Therapy Unit, Minimally Invasive Surgery Centre Jesus Uson , Cáceres , Spain.
authors.forename: Javier G
authors.initials: JG
authors.lastname: Casado
keywords: exosomes; immunomodulation; lymphocyte activation; lymphocytes; mesenchymal stem cells