title: Amine oxidase-based biosensors for spermine and spermidine determination.
abstract: The present work describes the development and optimization of electrochemical biosensors for specific determination of the biogenic polyamine spermine (Spm) and spermidine (Spmd) whose assessment represents a novel important analytical tool in food analysis and human diagnostics. These biosensors have been prepared using novel engineered enzymes: polyamine oxidase (PAO) endowed with selectivity towards Spm and Spmd and spermine oxidase (SMO) characterized by strict specificity towards Spm. The current design entails biosensors in which the enzymes were entrapped in poly(vinyl alcohol) bearing styrylpyridinium groups (PVA-SbQ), a photocrosslinkable gel, onto an electrode surface. Screen-printed electrodes (SPEs) were used as electrochemical transducers for enzymatically produced hydrogen peroxide, operating at different potential vs Ag/AgCl according to the material of the working electrode (WE): +700 mV for graphite (GP) or -100 mV for Prussian blue (PB)-modified SPE, respectively. Biosensor performances were evaluated by means of flow injection amperometric (FIA) measurements. The modified electrodes showed good sensitivity, long-term stability and reproducibility. Under optimal conditions, the PAO biosensor showed a linear range 0.003-0.3 mM for Spm and 0.01-0.4 mM for Spmd, while with the SMO biosensor, a linear range of 0.004-0.5 mM for Spm has been obtained. The main kinetic parameters apparent Michaelis constant (K M), turnover number (K cat) and steady-state current (I max) were determined. The proposed device was then applied to the determination of biogenic amines in blood samples. The results obtained were in good agreement with those obtained with the GC-MS reference method.
authors.affiliation: Department of Biochemical Sciences, Sapienza University of Rome, P.le Aldo Moro 5, 00185, Rome, Italy.
authors.forename: Alberto
authors.initials: A
authors.lastname: Boffi
authors.forename: Gabriele
authors.initials: G
authors.lastname: Favero
authors.forename: Rodolfo
authors.initials: R
authors.lastname: Federico
authors.forename: Alberto
authors.initials: A
authors.lastname: Macone
authors.forename: Riccarda
authors.initials: R
authors.lastname: Antiochia
authors.forename: Cristina
authors.initials: C
authors.lastname: Tortolini
authors.forename: Gabriella
authors.initials: G
authors.lastname: Sanzó
authors.forename: Franco
authors.initials: F
authors.lastname: Mazzei
mesh_terms: D006631:Amine Oxidase (Copper-Containing) / Q000378:metabolism*; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D004800:Enzymes, Immobilized / Q000378:metabolism*; D006801:Humans; D057230:Limit of Detection; D000587:Oxidoreductases Acting on CH-NH Group Donors / Q000378:metabolism; D011994:Recombinant Proteins; D013095:Spermidine / Q000032:analysis / Q000097:blood; D013096:Spermine / Q000032:analysis* / Q000097:blood; D013379:Substrate Specificity; D003313:Zea mays / Q000201:enzymology; D000097667:Polyamine Oxidase
chemical_list: D006631:Amine Oxidase (Copper-Containing); D004800:Enzymes, Immobilized; D000587:Oxidoreductases Acting on CH-NH Group Donors; D011994:Recombinant Proteins; D013095:Spermidine; D013096:Spermine; D000097667:Polyamine Oxidase