title: Rapid evaluation technique to differentiate mushroom disease-related moulds by detecting microbial volatile organic compounds using HS-SPME-GC-MS.
abstract: Headspace solid-phase microextraction (HS-SPME) coupled with gas chromatography-mass spectrometry (GC-MS) was used to analyse microbial volatile organic compounds (MVOCs) of mushroom disease-related microorganisms. Mycogone perniciosa, Lecanicillum fungicola var. fungicola, and Trichoderma aggressivum f. europaeum species, which are typically harmful in mushroom cultivation, were examined, and Agaricus bisporus (bisporic button mushroom) was also examined as a control. For internal standard, a mixture of alkanes was used; these were introduced as the memory effect of primed septa in the vial seal. Several different marker compounds were found in each sample, which enabled us to distinguish the different moulds and the mushroom mycelium from each other. Monitoring of marker compounds enabled us to investigate the behaviour of moulds. The records of the temporal pattern changes were used to produce partial least squares regression (PLS-R) models that enabled determination of the exact time of contamination (the infection time of the media). Using these evaluation techniques, the presence of mushroom disease-related fungi can be easily detected and monitored via their emitted MVOCs.
authors.affiliation: Department of Applied Chemistry, Faculty of Food Science, Corvinus 0University of Budapest, 1118, Budapest, Villányi út 29-43, Hungary.
authors.forename: Dalma
authors.initials: D
authors.lastname: Radványi
authors.forename: Attila
authors.initials: A
authors.lastname: Gere
authors.forename: Zsuzsa
authors.initials: Z
authors.lastname: Jókai
authors.forename: Péter
authors.initials: P
authors.lastname: Fodor
mesh_terms: D000363:Agaricales; D000473:Alkanes / Q000032:analysis; D015415:Biomarkers / Q000032:analysis; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006999:Hypocreales / Q000737:chemistry / Q000472:pathogenicity; D016018:Least-Squares Analysis; D010935:Plant Diseases / Q000382:microbiology*; D052617:Solid Phase Microextraction / Q000379:methods*; D013045:Species Specificity; D014242:Trichoderma / Q000737:chemistry / Q000472:pathogenicity; D020172:Verticillium / Q000737:chemistry / Q000472:pathogenicity; D055549:Volatile Organic Compounds / Q000032:analysis*
chemical_list: D000473:Alkanes; D015415:Biomarkers; D055549:Volatile Organic Compounds