title: Effect of dietary restriction on sperm characteristic and oxidative status on testicular tissue in young rats exposed to long-term heat stress.
abstract: This study was conducted to evaluate the effects of dietary restriction on oxidative status and sperm parameters in rats exposed to long-term heat stress. Forty healthy Sprague-Dawley rats, aged 2.5 month, were divided into four groups of 10 with respect to feeding and temperature regimen (room temperature (22 °C)-ad libitum, room temperature-dietary restriction (40%), high temperature (38 °C)-ad libitum, high temperature-dietary restriction). At the end of the 9th week, some oxidants (lipid hydroperoxide, total oxidant status, oxidative stress index) and antioxidants (total antioxidant status, sulfhydryl groups, ceruloplasmin, paraoxonase and arylesterase activities) were measured in the testis tissue. The concentration, motility, volume, abnormal sperm count, acrosome and membrane integrity of epididymal spermatozoon and intratesticular testosterone levels were evaluated. High temperature did not change oxidative and antioxidative parameters except for sulfhydryl groups and ceruloplasmin, yet it impaired all sperm values. Neither sperm values nor oxidative status apart from sulfhydryl groups, ceruloplasmin and arylesterase was affected by dietary restriction in the testis tissue. These results suggest that long-term heat stress does not have a significant effect on testicular oxidative status, while the spermatozoa are sensitive to heat stress in young rats. Dietary restriction failed to improve the sperm quality and oxidative status except some individual antioxidant parameters; conversely, it decreased intratesticular testosterone level in the young rats exposed to long-term heat stress.
authors.affiliation: Department of Physiology, Faculty of Veterinary Medicine, Harran University, Sanliurfa, Turkey.|Department of Physiology, Faculty of Veterinary Medicine, Dicle University, Diyarbakir, Turkey.
authors.forename: N
authors.initials: N
authors.lastname: Aydilek
authors.affiliation: Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Harran University, Sanliurfa, Turkey.
authors.forename: O
authors.initials: O
authors.lastname: Varisli
authors.affiliation: Department of Biochemistry, Faculty of Medicine, Harran University, Sanliurfa, Turkey.
authors.forename: A
authors.initials: A
authors.lastname: Kocyigit
authors.affiliation: Department of Biochemistry, Faculty of Medicine, Harran University, Sanliurfa, Turkey.
authors.forename: A
authors.initials: A
authors.lastname: Taskin
authors.affiliation: Department of Physiology, Faculty of Veterinary Medicine, Dicle University, Diyarbakir, Turkey.
authors.forename: M S
authors.initials: MS
authors.lastname: Kaya
mesh_terms: D000177:Acrosome; D000818:Animals; D043303:Aryldialkylphosphatase / Q000378:metabolism; D031204:Caloric Restriction; D002265:Carboxylic Ester Hydrolases / Q000378:metabolism; D002462:Cell Membrane; D002570:Ceruloplasmin / Q000378:metabolism; D006358:Hot Temperature; D008054:Lipid Peroxides / Q000378:metabolism; D008297:Male; D018384:Oxidative Stress / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013076:Sperm Count; D013081:Sperm Motility; D013094:Spermatozoa / Q000502:physiology*; D013737:Testis / Q000201:enzymology / Q000378:metabolism*; D013739:Testosterone / Q000097:blood
chemical_list: D008054:Lipid Peroxides; D013739:Testosterone; D002570:Ceruloplasmin; D002265:Carboxylic Ester Hydrolases; C019734:arylesterase; D043303:Aryldialkylphosphatase
keywords: Dietary restriction; heat stress; oxidative status; spermatozoon; testis