title: Molecular identification of a new begomovirus associated with leaf crumple disease of Jatropha curcas L. in India.
abstract: Association of a new begomovirus with leaf crumple disease of Jatropha curcas was identified based on sequence analysis of cloned ~2.7-kb viral DNA genomes from three representative samples amplified by RCA using phi-29 DNA polymerase. Sequence analysis of these isolates SKJ1 (KM066975), SKJ2 (KM189818) and SKJ3 (KM189819)--showed the presence of a monopartite begomovirus-like genome of 2,735 to 2,738 nucleotides containing seven ORFs: V3, V2 and V1 in virion sense and C3, C2, C1 and C4 in the complementary sense. Pairwise alignment showed 99 % nucleotide sequence similarity to each other and less than 81 % identity to other begomoviruses reported worldwide. Based on begomovirus species demarcation criteria for a new species (sequence identity <89 %), the begomovirus isolates were identified as the members of a new Begomovirus species and provisionally designated as Jatropha leaf crumple India virus (JLCrIV).
authors.affiliation: Plant Molecular Virology Laboratory, CPMB Division, CSIR-National Botanical Research Institute, Rana Pratap Marg, Lucknow, 226001, UP, India.
authors.forename: Ashish
authors.initials: A
authors.lastname: Srivastava
authors.forename: Meraj
authors.initials: M
authors.lastname: Jaidi
authors.forename: S
authors.initials: S
authors.lastname: Kumar
authors.forename: S K
authors.initials: SK
authors.lastname: Raj
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D053839:Begomovirus / Q000235:genetics* / Q000302:isolation & purification; D004279:DNA, Viral / Q000235:genetics; D016679:Genome, Viral / Q000235:genetics*; D007194:India; D031286:Jatropha / Q000821:virology*; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology*; D018515:Plant Leaves / Q000821:virology; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
chemical_list: D004279:DNA, Viral