title: Actinopolyspora biskrensis sp. nov., a novel halophilic actinomycete isolated from Northern Sahara.
abstract: A novel halophilic, filamentous actinomycete, designated H254(T), was isolated from a Saharan soil sample collected from Biskra (Northern Sahara), and subjected to a polyphasic taxonomic characterization. The strain is Gram-positive, aerobic, and halophilic, and the optimum NaCl concentration for growth is 15-20 % (w/v). The cell-wall hydrolysate contained meso-diaminopimelic acid, and the diagnostic whole-cell sugars were arabinose and galactose. The diagnostic phospholipid detected was phosphatidylcholine, and MK-9(H4) was the predominant menaquinone. The major fatty acid profiles were anteiso-C17:0 (32.8 %), C15:0 (28 %), and iso-C17:0 (12.3 %). Comparative analysis of the 16S rRNA gene sequences revealed that the strain H254(T) formed a well-separated sub-branch within the radiation of the genus Actinopolyspora, and the microorganism was most closely related to Actinopolyspora saharensis DSM 45459(T) (99.2 %), Actinopolyspora halophila DSM 43834(T) (99.1 %), and Actinopolyspora algeriensis DSM 45476(T) (99.0 %). Nevertheless, the strain had relatively lower mean values for DNA-DNA relatedness with the above strains (57.2, 68.4, and 45.6 %, respectively). Based on phenotypic features and phylogenetic position, we propose that strain H254(T) represents a novel species of the genus Actinopolyspora, for which the name Actinopolyspora biskrensis sp. nov. is proposed. The type strain of A. biskrensis is strain H254(T) (=DSM 46684(T) =CECT 8576(T)).
authors.affiliation: Laboratoire de Biologie des Systèmes Microbiens (LBSM), Ecole Normale Supérieure, Kouba, Alger, Algeria.
authors.forename: Rafika
authors.initials: R
authors.lastname: Saker
authors.forename: Noureddine
authors.initials: N
authors.lastname: Bouras
authors.forename: Atika
authors.initials: A
authors.lastname: Meklat
authors.forename: Abdelghani
authors.initials: A
authors.lastname: Zitouni
authors.forename: Peter
authors.initials: P
authors.lastname: Schumann
authors.forename: Cathrin
authors.initials: C
authors.lastname: Spröer
authors.forename: Hans-Peter
authors.initials: HP
authors.lastname: Klenk
authors.forename: Nasserdine
authors.initials: N
authors.lastname: Sabaou
mesh_terms: D000191:Actinomycetaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D000352:Africa, Northern; D055432:Metabolomics; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D012336:RNA, Ribosomal, 16S