title: Separation of Four Phenylpropanoid Glycosides from a Chinese Herb by HSCCC.
abstract: Four phenylpropanoid glycosides (PPGs), such as echinacoside, wiedemannioside C, forsythoside B and verbascoside, were isolated and purified from the Tibetan medicinal herb Pedicularis longiflora Rudolph. var. tubiformis (Klotz) Tsoong by high-speed counter-current chromatography (HSCCC) combined with macroporous resin (MR) column separation for the first time. In the present study, the two-phase solvent system composed of ethyl acetate-n-butanol-water (10 : 6:15, v/v/v) was used for HSCCC separation. A total of 3.5 mg of echinacoside, 12.6 mg of wiedemannioside C, 22.7 mg of forsythoside B and 48.7 mg of verbascoside with the purity of 93.6, 97.9, 97.8 and 98.1%, respectively, were obtained from 120 mg of crude sample. The HSCCC fractions were analyzed by HPLC, and the chemical structures were identified by (1)H NMR and (13)C NMR. The results demonstrate that MR coupled with HSCCC is a powerful technique for separation of PPGs from natural products.
authors.affiliation: Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810001, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810001, China Key Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810001, China.
authors.forename: Hui-Lan
authors.initials: HL
authors.lastname: Yue
authors.affiliation: Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810001, China Key Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810001, China Key Laboratory of Tibetan Medicine Research, Chinese Academy of Sciences, Xining 810001, China xhzhao@nwipb.ac.cn.
authors.forename: Xiao-Hui
authors.initials: XH
authors.lastname: Zhao
authors.affiliation: Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810001, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.affiliation: Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810001, China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Shao
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003377:Countercurrent Distribution / Q000379:methods*; D006027:Glycosides / Q000032:analysis / Q000737:chemistry* / Q000302:isolation & purification*; D059625:Liquid-Liquid Extraction; D032262:Pedicularis / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry*; D010946:Plants, Medicinal / Q000737:chemistry*
chemical_list: D006027:Glycosides; D010936:Plant Extracts