title: Genetic and serological characterization of chrysanthemum stem necrosis virus, a member of the genus Tospovirus.
abstract: Chrysanthemum stem necrosis virus (CSNV) is a member of a tentative tospovirus species. In this study, the complete genomic sequence of the Japanese CSNV isolate TcCh07A was determined. The L RNA is 8960 nt long and encodes the 331.0-kDa RNA-dependent RNA polymerase. The M RNA is 4828 nt long and encodes the 34.1-kDa movement protein (NSm) and the 127.7-kDa glycoprotein precursor (Gn/Gc). The S RNA is 2949 nt long and encodes the 52.4-kDa silencing suppressor protein (NSs) and the 29.3-kDa nucleocapsid (N) protein. The N protein of CSNV-TcCh07A was purified from virus-infected plant tissues and used for production of a rabbit polyclonal antiserum (RAs) and a monoclonal antibody (MAb). Results of serological tests by indirect ELISA and western blotting using the prepared RAs and MAb and a previously produced RAs against the N protein of tomato spotted wilt virus (TSWV) indicated that CSNV-TcCh07A, TSWV, tomato chlorotic spot virus, groundnut ringspot virus, alstroemeria necrotic streak virus and impatiens necrotic spot virus are serologically related.
authors.affiliation: Department of Biotechnology, Asia University, Wufeng, Taichung, 41354, Taiwan.
authors.forename: Pei-Ru
authors.initials: PR
authors.lastname: Wu
authors.forename: Wan-Chu
authors.initials: WC
authors.lastname: Chien
authors.forename: Mitsuru
authors.initials: M
authors.lastname: Okuda
authors.forename: Minoru
authors.initials: M
authors.lastname: Takeshita
authors.forename: Shyi-Dong
authors.initials: SD
authors.lastname: Yeh
authors.forename: Yun-Chi
authors.initials: YC
authors.lastname: Wang
authors.forename: Tsung-Chi
authors.initials: TC
authors.lastname: Chen
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D001483:Base Sequence; D031202:Chrysanthemum / Q000821:virology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D019590:Nucleocapsid Proteins / Q000276:immunology*; D010935:Plant Diseases / Q000821:virology*; D012367:RNA, Viral / Q000235:genetics*; D011817:Rabbits; D017423:Sequence Analysis, RNA; D017386:Sequence Homology, Amino Acid; D012698:Serologic Tests; D014026:Nicotiana / Q000821:virology; D017865:Tospovirus / Q000145:classification* / Q000235:genetics / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D019590:Nucleocapsid Proteins; D012367:RNA, Viral