title: Characterization of byproducts originating from hemp oil processing.
abstract: Valorization of hemp seed meal, a byproduct of hemp oil processing, was performed by measuring the distribution of nutritional and antinutritional compounds in different hemp seed meal fractions. According to chemical composition, two cotyledon-containing fractions (>180 and <180 μm) were significantly richer in protein (p < 0.05) (41.2% ± 0.04% and 44.4% ± 0.02%, respectively), lipid (15.1% ± 0.02% and 18.6% ± 0.04%, respectively), and sugar content (4.96% ± 0.11% and 3.46% ± 0.08%, respectively) in comparison to the hull-containing fractions (>350 and >250 μm), which were significantly richer in crude fiber content (29.5% ± 0.04% and 21.3% ± 0.03%, respectively). The free radical scavenging capacity (IC50) of fraction extracts increased (p < 0.05) with increasing mean particle size (from 17.18 ± 0.59 to 5.29 ± 0.30 mg/mL). Cannabisin B and N-trans-caffeoyltyramine were the most abundant phenolic compounds in the hull fractions (from 267 ± 15.9 to 287 ± 23.1 mg/kg), while cotyledon fractions had higher content of catechin (from 313 ± 12.4 to 744 ± 22.2 mg/kg) and p-hydroxybenzoic acid (from 124 ± 6.47 to 129 ± 8.56 mg/kg (P < 0.05). Well-balanced ω-6 to ω-3 fatty acid ratio (3:1) was determined in all fractions. Antinutrients (trypsin inhibitors, phytic acid, glucosinolates, and condensed tannins) were mostly located in the cotyledon fractions. These findings indicate that the separation of hemp seed meal into different fractions could be used to concentrate valuable target compounds and consequently facilitate their recovery.
authors.affiliation: Institute of Food Technology, University of Novi Sad , Bulevar cara Lazara 1, 21000 Novi Sad, Serbia.
authors.forename: Milica
authors.initials: M
authors.lastname: Pojić
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Mišan
authors.forename: Marijana
authors.initials: M
authors.lastname: Sakač
authors.forename: Tamara
authors.initials: T
authors.lastname: Dapčević Hadnađev
authors.forename: Bojana
authors.initials: B
authors.lastname: Šarić
authors.forename: Ivan
authors.initials: I
authors.lastname: Milovanović
authors.forename: Miroslav
authors.initials: M
authors.lastname: Hadnađev
mesh_terms: D002188:Cannabis / Q000737:chemistry* / Q000196:embryology; D002241:Carbohydrates / Q000737:chemistry; D018548:Cotyledon / Q000737:chemistry; D016166:Free Radical Scavengers / Q000737:chemistry; D008055:Lipids / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry*; D010938:Plant Oils / Q000737:chemistry*; D010940:Plant Proteins / Q000737:chemistry; D012639:Seeds / Q000737:chemistry / Q000196:embryology; D014361:Trypsin Inhibitors / Q000737:chemistry; D014866:Waste Products / Q000032:analysis*
chemical_list: D002241:Carbohydrates; D016166:Free Radical Scavengers; D008055:Lipids; D010936:Plant Extracts; D010938:Plant Oils; D010940:Plant Proteins; D014361:Trypsin Inhibitors; D014866:Waste Products
keywords: antioxidant potential; fractionation; hemp meal; nutritive and antinutritive value