title: Effects of histidine and n-acetylcysteine on experimental lesions induced by doxorubicin in sciatic nerve of rats.
abstract: In this study, the effect of separate and combined intraperitoneal (i.p.) injections of histidine and n-acetylcysteine were investigated on experimental damage induced by doxorubicin (DOX) in sciatic nerve of rats. DOX was i.p. injected at a dose of 4 mg/kg once weekly for four weeks. Histidine and n-acetylcysteine were i.p. injected at a same dose of 20 mg/kg. Cold and mechanical allodynia were recorded using acetone spray and von Frey filaments tests, respectively. The sciatic nerve damage was evaluated by light microscopy. Plasma levels of malondialdehyde (MDA) and total antioxidant capacity (TAC) were measured. Histidine and especially n-acetylcysteine at a same dose of 20 mg/kg suppressed cold and mechanical allodynia, improved sciatic nerve lesions and reversed MDA and TAC levels in DOX-treated groups. Combination treatment with histidine and n-acetylcysteine showed better responses when compared with them used alone. The results of the present study showed peripheral neuroprotective effects for histidine and n-acetylcysteine. Reduction of free radical-induced toxic effects may have a role in neuroprotective properties of histidine and n-acetylcysteine.
authors.affiliation: a Division of Pathology, Department of Pathobiology, Faculty of Veterinary Medicine and.
authors.forename: Amir Abbas
authors.initials: AA
authors.lastname: Farshid
authors.affiliation: b Division of Physiology, Department of Basic Sciences, Faculty of Veterinary Medicine , Urmia University , Urmia , Iran.
authors.forename: Esmaeal
authors.initials: E
authors.lastname: Tamaddonfard
authors.affiliation: a Division of Pathology, Department of Pathobiology, Faculty of Veterinary Medicine and.
authors.forename: Sima
authors.initials: S
authors.lastname: Najafi
mesh_terms: D000111:Acetylcysteine / Q000008:administration & dosage / Q000494:pharmacology*; D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000633:toxicity; D000975:Antioxidants / Q000378:metabolism; D004317:Doxorubicin / Q000633:toxicity*; D004359:Drug Therapy, Combination; D005609:Free Radicals / Q000378:metabolism; D006639:Histidine / Q000008:administration & dosage / Q000494:pharmacology*; D007274:Injections, Intraperitoneal; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D018696:Neuroprotective Agents / Q000008:administration & dosage / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D012584:Sciatic Nerve / Q000187:drug effects / Q000473:pathology; D020426:Sciatic Neuropathy / Q000139:chemically induced / Q000517:prevention & control
chemical_list: D000903:Antibiotics, Antineoplastic; D000975:Antioxidants; D005609:Free Radicals; D018696:Neuroprotective Agents; D006639:Histidine; D008315:Malondialdehyde; D004317:Doxorubicin; D000111:Acetylcysteine
keywords: Doxorubicin; histidine; n-acetylcysteine; rats; sciatic nerve