title: Activation of Cryptic hop Genes from Streptomyces peucetius ATCC 27952 Involved in Hopanoid Biosynthesis.
abstract: Genes encoding enzymes with sequence similarity to hopanoids biosynthetic enzymes of other organisms were cloned from the hopanoid (hop) gene cluster of Streptomyces peucetius ATCC 27952 and transformed into Streptomyces venezuelae YJ028. The cloned fragments contained four genes, all transcribed in one direction. These genes encode polypeptides that resemble polyprenyl diphosphate synthase (hopD), squalene-phytoene synthases (hopAB), and squalenehopene cyclase (hopE). These enzymes are sufficient for the formation of the pentacyclic triterpenoid lipid, hopene. The formation of hopene was verified by gas chromatography/ mass spectrometry.
authors.affiliation: Institute of Biomolecule Reconstruction (iBR), Department of BT-convergent Pharmaceutical Engineering, Sun Moon University, Asansi 336-708, Republic of Korea.
authors.forename: Gopal Prasad
authors.initials: GP
authors.lastname: Ghimire
authors.affiliation: Institute of Biomolecule Reconstruction (iBR), Department of BT-convergent Pharmaceutical Engineering, Sun Moon University, Asansi 336-708, Republic of Korea.
authors.forename: Niranjan
authors.initials: N
authors.lastname: Koirala
authors.affiliation: Institute of Biomolecule Reconstruction (iBR), Department of BT-convergent Pharmaceutical Engineering, Sun Moon University, Asansi 336-708, Republic of Korea.
authors.forename: Jae Kyung
authors.initials: JK
authors.lastname: Sohng
mesh_terms: D003001:Cloning, Molecular; D005798:Genes, Bacterial / Q000235:genetics*; D005810:Multigene Family / Q000235:genetics*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D013302:Streptomyces / Q000235:genetics* / Q000378:metabolism; D014315:Triterpenes / Q000378:metabolism*
chemical_list: D011994:Recombinant Proteins; D014315:Triterpenes