title: Sterols from Mytilidae show anti-aging and neuroprotective effects via anti-oxidative activity.
abstract: For screening anti-aging samples from marine natural products, K6001 yeast strain was employed as a bioassay system. The active mussel extract was separated to give an active sterol fraction (SF). SF was further purified, and four sterol compounds were obtained. Their structures were determined to be cholesterol (CHOL), brassicasterol, crinosterol, and 24-methylenecholesterol. All compounds showed similar anti-aging activity. To understand the action mechanism involved, anti-oxidative experiments, reactive oxygen species (ROS) assays, and malondialdehyde (MDA) tests were performed on the most abundant compound, CHOL. Results indicated that treatment with CHOL increases the survival rate of yeast under oxidative stress and decreases ROS and MDA levels. In addition, mutations of uth1, skn7, sod1, and sod2, which feature a K6001 background, were employed and the lifespans of the mutations were not affected by CHOL. These results demonstrate that CHOL exerts anti-aging effects via anti-oxidative stress. Based on the connection between neuroprotection and anti-aging, neuroprotective experiments were performed in PC12 cells. Paraquat was used to induce oxidative stress and the results showed that the CHOL and SF protect the PC12 cells from the injury induced by paraquat. In addition, these substance exhibited nerve growth factor (NGF) mimic activities again confirmed their neuroprotective function.
authors.affiliation: College of Pharmaceutical Sciences, Zhejiang University, Yu Hang Tang Road 866, Hangzhou 310058, China. 11119014@zju.edu.cn.
authors.forename: Yujuan
authors.initials: Y
authors.lastname: Sun
authors.affiliation: College of Pharmaceutical Sciences, Zhejiang University, Yu Hang Tang Road 866, Hangzhou 310058, China. 11219008@zju.edu.cn.
authors.forename: Yanfei
authors.initials: Y
authors.lastname: Lin
authors.affiliation: College of Pharmaceutical Sciences, Zhejiang University, Yu Hang Tang Road 866, Hangzhou 310058, China. 11319006@zju.edu.cn.
authors.forename: Xueli
authors.initials: X
authors.lastname: Cao
authors.affiliation: College of Pharmaceutical Sciences, Zhejiang University, Yu Hang Tang Road 866, Hangzhou 310058, China. lxiang@zju.edu.cn.
authors.forename: Lan
authors.initials: L
authors.lastname: Xiang
authors.affiliation: College of Pharmaceutical Sciences, Zhejiang University, Yu Hang Tang Road 866, Hangzhou 310058, China. qijianhua@zju.edu.cn.
authors.forename: Jianhua
authors.initials: J
authors.lastname: Qi
mesh_terms: D000375:Aging / Q000187:drug effects*; D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects; D008315:Malondialdehyde / Q000378:metabolism; D009154:Mutation / Q000235:genetics; D049879:Mytilidae / Q000737:chemistry*; D018696:Neuroprotective Agents / Q000737:chemistry / Q000494:pharmacology*; D010084:Oxidation-Reduction / Q000187:drug effects; D016716:PC12 Cells; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000187:drug effects; D013261:Sterols / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; D018696:Neuroprotective Agents; D017382:Reactive Oxygen Species; D013261:Sterols; D008315:Malondialdehyde