title: Regulatory role of transcription factor SutR (YdcN) in sulfur utilization in Escherichia coli.
abstract: Sulfur makes up 1 % of the dry mass of bacteria, and it is an abundant element (0.1 %) on earth. Sulfur in the environment is, however, mostly in oxidized forms and inaccessible to living organisms. At present, the entire assimilation pathway of external sulfur to sulfur-containing biomolecules and its regulation in Escherichia coli remain poorly understood, except for the metabolic pathway of cysteine synthesis, the first-step metabolite of sulfur assembly. During the search for regulation targets of uncharacterized transcription factors by Genomic SELEX screening, we found that the hitherto uncharacterized YdcN regulates a set of genes involved in the utilization of sulfur, including the generation of sulfate and its reduction, the synthesis of cysteine, the synthesis of enzymes containing Fe-S as cofactors, and the modification of tRNA with use of sulfur-containing substrates. Taking these findings together, we propose renaming YdcN as SutR (regulator of sulfur utilization).
authors.affiliation: Research Institute of Micro-Nano Technology, Hosei University, Koganei, Tokyo 185-8584, Japan.|Department of Frontier Bioscience, Hosei University, Koganei, Tokyo 185-8584, Japan.
authors.forename: Kaneyoshi
authors.initials: K
authors.lastname: Yamamoto
authors.affiliation: Research Institute of Micro-Nano Technology, Hosei University, Koganei, Tokyo 185-8584, Japan.
authors.forename: Masahiro
authors.initials: M
authors.lastname: Nakano
authors.affiliation: Research Institute of Micro-Nano Technology, Hosei University, Koganei, Tokyo 185-8584, Japan.|Department of Frontier Bioscience, Hosei University, Koganei, Tokyo 185-8584, Japan.
authors.forename: Akira
authors.initials: A
authors.lastname: Ishihama
mesh_terms: D001483:Base Sequence; D001665:Binding Sites; D021901:DNA, Intergenic; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D023061:Gene Order; D053263:Gene Regulatory Networks; D023281:Genomics; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D015203:Reproducibility of Results; D052156:SELEX Aptamer Technique; D013455:Sulfur / Q000378:metabolism*; D014157:Transcription Factors / Q000378:metabolism*; D024363:Transcription Initiation Site; D014158:Transcription, Genetic
chemical_list: D021901:DNA, Intergenic; D029968:Escherichia coli Proteins; D014157:Transcription Factors; D013455:Sulfur