title: Profiling the metabolism of astragaloside IV by ultra performance liquid chromatography coupled with quadrupole/time-of-flight mass spectrometry.
abstract: Astragaloside IV is a compound isolated from the Traditional Chinese Medicine Astragalus membranaceus, that has been reported to have bioactivities against cardiovascular disease and kidney disease. There is limited information on the metabolism of astragaloside IV, which impedes comprehension of its biological actions and pharmacology. In the present study, an ultra-performance liquid chromatography coupled with quadrupole/time-of-flight mass spectrometry (UPLC-Q-TOF-MS/MS)-based approach was developed to profile the metabolites of astragaloside IV in rat plasma, bile, urine and feces samples. Twenty-two major metabolites were detected. The major components found in plasma, bile, urine and feces included the parent chemical and phases I and II metabolites. The major metabolic reactions of astragaloside IV were hydrolysis, glucuronidation, sulfation and dehydrogenation. These results will help to improve understanding the metabolism and reveal the biotransformation profiling of astragaloside IV in vivo. The metabolic information obtained from our study will guide studies into the pharmacological activity and clinical safety of astragaloside IV.
authors.affiliation: College of Pharmacy, Nanjing University of Chinese Medicine, No. 218 Xianlin Avenue, Nanjing 210046, China. chengxd2013@gmail.com.
authors.forename: Xu-Dong
authors.initials: XD
authors.lastname: Cheng
authors.affiliation: The First Affiliated Hospital of Soochow University, No. 188 Shizi Street, Suzhou 215006, China. weiminggang@suda.edu.cn.
authors.forename: Ming-Gang
authors.initials: MG
authors.lastname: Wei
mesh_terms: D000818:Animals; D027885:Astragalus propinquus / Q000737:chemistry; D001646:Bile / Q000737:chemistry / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D005243:Feces / Q000737:chemistry; D008297:Male; D008516:Medicine, Chinese Traditional; D051381:Rats; D017207:Rats, Sprague-Dawley; D012503:Saponins / Q000097:blood / Q000378:metabolism* / Q000652:urine; D053719:Tandem Mass Spectrometry / Q000379:methods; D014315:Triterpenes / Q000097:blood / Q000378:metabolism* / Q000652:urine
chemical_list: D004365:Drugs, Chinese Herbal; D012503:Saponins; D014315:Triterpenes; C052064:astragaloside A