title: Osteogenic Differentiation from Embryonic Stem Cells.
abstract: Embryonic stem (ES) cells have been widely studied due to their pluripotency and their potential of self-renewal. Murine ES cells are useful in investigating the molecular pathways underlying their differentiation to various mature cell types in the body. This chapter describes the maintenance of murine ES cells in culture and a routine ES cell osteogenic differentiation protocol utilized in our laboratory.
authors.affiliation: Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, ON, Canada, M5S 1A8.
authors.forename: Yanhong
authors.initials: Y
authors.lastname: Yu
authors.affiliation: Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, ON, Canada, M5S 1A8.
authors.forename: Carlos
authors.initials: C
authors.lastname: Pilquil
authors.affiliation: Department of Laboratory Medicine and Pathobiology, University of Toronto, 1 King's College Circle, Medical Sciences Building, Room 6326, Toronto, ON, Canada, M5S 1A8. m.opas@utoronto.ca.
authors.forename: Michal
authors.initials: M
authors.lastname: Opas
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation; D049109:Cell Proliferation; D058732:Embryoid Bodies / Q000166:cytology*; D053595:Embryonic Stem Cells / Q000166:cytology*; D051379:Mice; D010012:Osteogenesis; D046509:Tissue Culture Techniques / Q000379:methods*
keywords: Embryoid bodies; Hanging drop; Murine embryonic stem cells; Osteogenesis; Routine culture