title: Effect of fibroblast growth factor 9 on the osteogenic differentiation of bone marrow stromal stem cells and dental pulp stem cells.
abstract: The role of fibroblast growth factor 9 (FGF9) in bone formation may depend on gene dosage, developmental stage, cell type or interactions with other cytokines. In the present study bone marrow stromal stem cells (BMSCs) and dental pulp stem cells (DPSCs) were cultured and osteogenically induced in vitro, treated with exogenous FGF9 at varying concentrations. Alkaline phosphatase staining, alizarin red S staining, reverse transcription quantitative polymerase chain reaction and western blot analyses were performed in order to investigate the gene expression levels of osteogenic markers. The results of the present study demonstrated that FGF9 enhanced the phosphorylation of extracellular signal‑regulated kinase 1/2 (ERK1/2) during osteogenic induction in BMSCs and DPSCs, which are derived from different tissues. FGF9 also inhibited the osteogenic differentiation of BMSCs and DPSCs through the activation of ERK1/2. These findings suggested that FGF9 may be an inhibitor of osteogenesis in mesenchymal stem cells in vitro and its application in vivo requires investigation in the future.
authors.affiliation: Department of Oral and Craniomaxillofacial Science, Shanghai Ninth People's Hospital College of Stomatology, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, P.R. China.
authors.forename: Jingting
authors.initials: J
authors.lastname: Lu
authors.affiliation: Department of Oral and Craniomaxillofacial Science, Shanghai Ninth People's Hospital College of Stomatology, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, P.R. China.
authors.forename: Jiewen
authors.initials: J
authors.lastname: Dai
authors.affiliation: Department of Oral and Craniomaxillofacial Science, Shanghai Ninth People's Hospital College of Stomatology, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, P.R. China.
authors.forename: Xudong
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Oral and Craniomaxillofacial Science, Shanghai Ninth People's Hospital College of Stomatology, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, P.R. China.
authors.forename: Maolin
authors.initials: M
authors.lastname: Zhang
authors.affiliation: Department of Oral and Craniomaxillofacial Science, Shanghai Ninth People's Hospital College of Stomatology, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, P.R. China.
authors.forename: Peng
authors.initials: P
authors.lastname: Zhang
authors.affiliation: Department of Oral and Craniomaxillofacial Science, Shanghai Ninth People's Hospital College of Stomatology, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, P.R. China.
authors.forename: Hao
authors.initials: H
authors.lastname: Sun
authors.affiliation: Oral Bioengineering Lab, Shanghai Key Laboratory of Stomatology, Shanghai Research Institute of Stomatology, Shanghai Ninth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai 200011, P.R. China.
authors.forename: Xiuli
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Oral and Craniomaxillofacial Science, Shanghai Ninth People's Hospital College of Stomatology, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, P.R. China.
authors.forename: Hongbo
authors.initials: H
authors.lastname: Yu
authors.affiliation: Department of Oral and Craniomaxillofacial Science, Shanghai Ninth People's Hospital College of Stomatology, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, P.R. China.
authors.forename: Wenbin
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Department of Oral and Craniomaxillofacial Science, Shanghai Ninth People's Hospital College of Stomatology, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, P.R. China.
authors.forename: Lei
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Oral Bioengineering Lab, Shanghai Key Laboratory of Stomatology, Shanghai Research Institute of Stomatology, Shanghai Ninth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai 200011, P.R. China.
authors.forename: Xinquan
authors.initials: X
authors.lastname: Jiang
authors.affiliation: Department of Oral and Craniomaxillofacial Science, Shanghai Ninth People's Hospital College of Stomatology, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, P.R. China.
authors.forename: Steve Guofang
authors.initials: SG
authors.lastname: Shen
mesh_terms: D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D003782:Dental Pulp / Q000166:cytology*; D051525:Fibroblast Growth Factor 9 / Q000494:pharmacology*; D006801:Humans; D048031:JNK Mitogen-Activated Protein Kinases / Q000378:metabolism; D020935:MAP Kinase Signaling System / Q000187:drug effects; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects* / Q000378:metabolism; D019950:Mitogen-Activated Protein Kinase 1 / Q000378:metabolism; D048052:Mitogen-Activated Protein Kinase 3 / Q000378:metabolism; D010012:Osteogenesis / Q000187:drug effects*; D010766:Phosphorylation; D051381:Rats; D013234:Stem Cells / Q000166:cytology* / Q000187:drug effects* / Q000378:metabolism; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism
chemical_list: D051525:Fibroblast Growth Factor 9; D048031:JNK Mitogen-Activated Protein Kinases; D019950:Mitogen-Activated Protein Kinase 1; D048052:Mitogen-Activated Protein Kinase 3; D048051:p38 Mitogen-Activated Protein Kinases