title: Er-Xian Decoction, a traditional Chinese herbal formula, intervening early in hypothalamic-pituitary axis of male rats with delayed puberty.
abstract: BACKGROUND
  Er-Xian Decoction (EXD) is one of the traditional Chinese medicine (TCM) with unique effect on osteoporosis, menopausal syndrome and delayed puberty in China for many years.
  
  
  OBJECTIVE
  We aim to evaluate the potential activity of starting hypothalamic-pituitary-testicular (HPT) axis of male rats with delayed puberty.
  
  
  METHODS
  Delayed puberty model of male Sprague-Dawley (SD) rats were established with soy isoflavones (90 mg·kg(-1)) and were treated by EXD extract at doses of 5, 10 g·kg(-1) or Testosterone undecanoate (TU) for 8 weeks. Body weight, body length, testis weight, T, E2 and luteinizing hormone (LH) in serum, gonadotropin releasing hormone (GnRH) in hypothalamus, follicle stimulating hormone (FSH) and LH in pituitary gland were determined by ELISA. Immunohistochemistry was used to detect LH in pituitary gland.
  
  
  RESULTS
  Soy isoflavones could significantly decrease body weight, body length, testicular organ coefficient T in serum, GnRH in hypothalamus, FSH and LH in pituitary gland. Both of EXD and TU could improve the condition. E2 and LH in serum of all groups were non-significance of difference (P > 0.05). The immunohistochemical results were well consistent with LH in pituitary gland.
  
  
  CONCLUSIONS
  The results of the present research indicate that EXD extract is effective to start the HPT axis in puberty and can significantly improve sexual developmental inhibition caused by soy isoflavones.
authors.affiliation: Department of Pharmacognosy, School of Pharmacy, China Medical University, Shenyang, PR China.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Zhu
authors.affiliation: Department of Pharmacognosy, School of Pharmacy, China Medical University, Shenyang, PR China.
authors.forename: LiHong
authors.initials: L
authors.lastname: Li
authors.affiliation: Department of Pharmacognosy, School of Pharmacy, China Medical University, Shenyang, PR China.
authors.forename: Xin
authors.initials: X
authors.lastname: Jin
authors.affiliation: Department of Pharmacognosy, School of Pharmacy, China Medical University, Shenyang, PR China.
authors.forename: JianWei
authors.initials: J
authors.lastname: Fang
authors.affiliation: Department of Pharmacognosy, School of Pharmacy, China Medical University, Shenyang, PR China.
authors.forename: DongFang
authors.initials: D
authors.lastname: Zhang
keywords: Delayed puberty; Er-Xian Decoction; traditional Chinese medicine