title: In vitro anti-cataract evaluation of standardised Abies pindrow leaf extract using isolated goat lenses.
abstract: Oxidative stress is known to spark off the pathogenesis of cataract. Antioxidant potential of Abies pindrow Royle leaf extract (APE) is well established in the literature. In this context, standard aqueous leaf extract of this plant was evaluated for its role in hydrogen peroxide-induced cataract in isolated goat lenses using varying concentrations (5, 10, 15 and 20 mg/mL). Total phenol and flavonoidal content was evaluated and found to be high in concentration. Biochemical parameters, namely superoxide dismutase (SOD), glutathione (GSH), total protein content (TPC) and malondialdehyde (MDA), were evaluated. SOD, GSH and TPC formation was found to increase proportionally with increasing concentration. However, MDA level decreased significantly as the concentration of the extract increased. The results suggest that the extract under investigation can delay the onset and/or prevent the progression of cataract. Its anti-cataract potential may be attributed to the presence of high phenolics and flavonoids in APE. Photographic evaluation, further, confirmed the observation.
authors.affiliation: a Amity Institute of Pharmacy, Amity University, Lucknow Campus , Lucknow , Uttar Pradesh , India.
authors.forename: Suchita
authors.initials: S
authors.lastname: Dubey
authors.forename: Sudipta
authors.initials: S
authors.lastname: Saha
authors.forename: Shubhini A
authors.initials: SA
authors.lastname: Saraf
mesh_terms: D028202:Abies / Q000737:chemistry*; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002386:Cataract / Q000188:drug therapy*; D005419:Flavonoids / Q000494:pharmacology; D005979:Glutathione Peroxidase / Q000378:metabolism; D006041:Goats; D066298:In Vitro Techniques; D007908:Lens, Crystalline / Q000187:drug effects*; D008315:Malondialdehyde / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D010636:Phenols / Q000494:pharmacology; D010936:Plant Extracts / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D000975:Antioxidants; D005419:Flavonoids; D010636:Phenols; D010936:Plant Extracts; D008315:Malondialdehyde; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase
keywords: Abies pindrow; antioxidant; cataract; hydrogen peroxide