title: Two-photon multiplexing bio-imaging using a combination of Eu- and Tb-bioprobes.
abstract: During the course of our research studies aiming to combine the unique photophysical properties of lanthanide complexes and the intrinsic advantage of a nonlinear two-photon excitation, we described the proof-of-concept of a biphotonic multiplexing bio-imaging experiment. To that end, a new Tb-luminescent bioprobe [TbL(2)] was designed using the TACN-trispicolinate platform. The biphotonic imaging of T24 fixed cells stained either with [TbL(2)] or with the already described [EuL(1)] complexes is reported. Finally the biphotonic multiplexing experiment was performed using a combination of both probes using the spectral mode of the two-photon microscope.
authors.affiliation: Laboratoire de Chimie UMR UMR 5182 CNRS-University Lyon- ENS Lyon, 46 allée d'Italie, 69364 Lyon, France. olivier.maury@ens-lyon.fr.
authors.forename: Virginie
authors.initials: V
authors.lastname: Placide
authors.forename: Anh Thy
authors.initials: AT
authors.lastname: Bui
authors.forename: Alexei
authors.initials: A
authors.lastname: Grichine
authors.forename: Alain
authors.initials: A
authors.lastname: Duperray
authors.forename: Delphine
authors.initials: D
authors.lastname: Pitrat
authors.forename: Chantal
authors.initials: C
authors.lastname: Andraud
authors.forename: Olivier
authors.initials: O
authors.lastname: Maury
mesh_terms: D045744:Cell Line, Tumor; D005063:Europium / Q000737:chemistry*; D006801:Humans; D049408:Luminescent Agents / Q000737:chemistry; D057054:Molecular Imaging / Q000379:methods*; D017785:Photons; D013725:Terbium / Q000737:chemistry*
chemical_list: D049408:Luminescent Agents; D013725:Terbium; D005063:Europium