title: Inhibition of calcification of bovine pericardium after treatment with biopolymers, E-beam irradiation and in vitro endothelization.
abstract: This work has investigated the in vitro calcification of bovine pericardium (BP) treated with chitosan (C), silk fibroin (SF) and electron beam irradiation after its endothelization in vitro. For this purpose, freeze-dried BP membranes treated with mixtures of C and SF (1:3, 1:1 and 3:1) and then irradiated by electron beam irradiation were seeded with human umbilical vein endothelial cells (HUVEC) in vitro. After 3 weeks of cultivation these membranes were submitted to in vitro calcification tests using simulated body fluid as the calcifying agent. Control membranes were also studied (without endothelial cells exposure). The results have shown that the membrane compatibility with HUVECs in vitro prevent such biomaterial from calcifying, showing a potential application in biomaterial area, such as cardiac valves and repair patches.
authors.affiliation: Department of Biochemical and Pharmaceutical Technology, School of Pharmaceutical Sciences, University of São Paulo, USP, São Paulo, SP, Brazil.
authors.forename: Roberta
authors.initials: R
authors.lastname: Polak
authors.affiliation: Biotechnology Center, Energy and Nuclear Research Institute, IPEN-CNEN/SP, São Paulo, SP, Brazil.
authors.forename: Andrea C D
authors.initials: AC
authors.lastname: Rodas
authors.affiliation: Department of Chemical Engineering of Polytechnic School, University of São Paulo, SP, Brazil.
authors.forename: Dennis L
authors.initials: DL
authors.lastname: Chicoma
authors.affiliation: Department of Chemical Engineering of Polytechnic School, University of São Paulo, SP, Brazil.
authors.forename: Reinaldo
authors.initials: R
authors.lastname: Giudici
authors.affiliation: School of Chemical Engineering, University of Campinas, UNICAMP, Campinas, SP, Brazil.
authors.forename: Marisa M
authors.initials: MM
authors.lastname: Beppu
authors.affiliation: Biotechnology Center, Energy and Nuclear Research Institute, IPEN-CNEN/SP, São Paulo, SP, Brazil.
authors.forename: Olga Z
authors.initials: OZ
authors.lastname: Higa
authors.affiliation: Department of Biochemical and Pharmaceutical Technology, School of Pharmaceutical Sciences, University of São Paulo, USP, São Paulo, SP, Brazil. Electronic address: pitombo@usp.br.
authors.forename: Ronaldo N M
authors.initials: RN
authors.lastname: Pitombo
mesh_terms: D000818:Animals; D002113:Calcification, Physiologic / Q000187:drug effects*; D002417:Cattle; D048271:Chitosan / Q000494:pharmacology*; D004583:Electrons; D004727:Endothelium / Q000187:drug effects / Q000502:physiology*; D005349:Fibroins / Q000494:pharmacology*; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D010496:Pericardium / Q000187:drug effects / Q000502:physiology*; D013052:Spectrometry, X-Ray Emission; D013059:Spectrum Analysis, Raman
chemical_list: D005349:Fibroins; D048271:Chitosan