title: The diversity of major histocompatibility complex class II DRB1 gene in sheep breeds from Xinjiang, China.
abstract: Exon 2 of the ovine leukocyte antigen OLA-DRB1 locus was examined in sheep from the Xinjiang Karakul Ram and Bashibai populations, and three generations of hybrids were derived from a cross between Bashibai and Altai Argali wild sheep. This identified 12 novel alleles and 30 previously reported alleles. A neighbor-joining tree of the amino acid sequences of these 42 alleles revealed allelic clusters shared across the study populations. There were significant differences in allelic frequency between Karakul Ram and Bashibai sheep. DRB1*K18cC was the most frequent allele in Kararul Ram with a frequency of 21.2%, while DRB1*2F10c8 (13.2%) and DRB1*0803 (13.2%) were the most frequent alleles found in Bashibai sheep; the alleles DRB1*2F16c2, DRB1*1601, and DRB1*0803 occurred most frequently in F1, F2, and F3 populations, with frequencies of 17.6%, 14.3%, and 20%, respectively. Although many alleles were shared by Bashibai and hybrid sheep, some alleles differed between them, especially in the F1 generation of the Bashibai × Altai Argali cross. The hybrid-specific alleles indicated the introgression of Altai Argali alleles into hybrid flocks. A population tree based on the OLA-DRB1 allelic frequency in each population indicated that the Bashibai sheep and three hybrid populations were similar, with Karakul Ram being genetically distinct.
authors.affiliation: College of Life Sciences and Technology, Xinjiang University, Urumqi, China; Viral Infectious Diseases Unit, RIKEN, Wako, Japan; Laboratory of Viral Infectious Diseases, Department of Medical Genome Sciences, Graduate School of Frontier Science, The University of Tokyo, Wako, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Polat
authors.forename: Y
authors.initials: Y
authors.lastname: Aida
authors.forename: S-N
authors.initials: SN
authors.lastname: Takeshima
authors.forename: J
authors.initials: J
authors.lastname: Aniwashi
authors.forename: M
authors.initials: M
authors.lastname: Halik
mesh_terms: D000483:Alleles; D000818:Animals; D001483:Base Sequence; D001947:Breeding; D002678:Chimera / Q000235:genetics* / Q000276:immunology; D002681:China; D003433:Crosses, Genetic; D005091:Exons; D005260:Female; D005787:Gene Frequency; D014644:Genetic Variation; D000949:Histocompatibility Antigens Class II / Q000145:classification / Q000235:genetics* / Q000276:immunology; D008297:Male; D008969:Molecular Sequence Data; D010802:Phylogeny; D012756:Sheep
chemical_list: D000949:Histocompatibility Antigens Class II
keywords: Altai Argali wild sheep; Bashibai sheep; Karakul Ram; OLA-DRB1; phylogenetic tree