title: Bioengineered post-natal recombinant tooth bud models.
abstract: The long-term goal of this study is to devise reliable methods to regenerate full-sized and fully functional biological teeth in humans. In this study, three-dimensional (3D) tissue engineering methods were used to characterize intact postnatal dental tissue recombinant constructs, and dental cell suspension recombinant constructs, as models for bioengineered tooth development. In contrast to studies using mouse embryonic dental tissues and cells, here the odontogenic potential of intact dental tissues and dental cell suspensions harvested from post natal porcine teeth and human third molar wisdom tooth dental pulp were examined. The recombinant 3D tooth constructs were cultured in osteogenic media in vitro for 1 week before subcutaneous transplantation in athymic nude rat hosts for 1 month or 3 months. Subsequent analyses using X-ray, histological and immunohistochemical methods showed that the majority of the recombinant tooth structures formed calcified tissues, including osteodentin, dentin cementum, enamel and morphologically typical tooth crowns composed of dentin and enamel. The demonstrated formation of mineralized dental tissues and tooth crown structures from easily obtained post-natal dental tissues is an important step toward reaching the long-term goal of establishing robust and reliable models for human tooth regeneration. Copyright © 2014 John Wiley & Sons, Ltd.
authors.affiliation: Department of Oral and Maxillofacial Pathology, Tufts University, Boston, MA, USA.
authors.forename: W
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Department of Oral and Maxillofacial Pathology, Tufts University, Boston, MA, USA.
authors.forename: B
authors.initials: B
authors.lastname: Vázquez
authors.affiliation: Department of Oral and Maxillofacial Pathology, Tufts University, Boston, MA, USA.
authors.forename: P C
authors.initials: PC
authors.lastname: Yelick
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D057005:Bioengineering / Q000379:methods*; D005455:Fluorescent Antibody Technique; D006801:Humans; D020007:Implants, Experimental; D023421:Models, Animal; D040521:Subcutaneous Tissue / Q000473:pathology; D034421:Sus scrofa; D054457:Tissue Scaffolds; D014083:Tooth Germ / Q000502:physiology*
keywords: adult stem cells; bioengineering; tissue recombinants; tooth bud