title: Turning an aptamer into a light-switch probe with a single bioconjugation.
abstract: We describe a method for transforming a structure-switching aptamer into a luminescent light-switch probe via a single conjugation. The methodology is demonstrated using a known aptamer for Hg(2+) as a case study. This approach utilizes a lanthanide-based metallointercalator, Eu-DOTA-Phen, whose luminescence is quenched almost entirely and selectively by purines, but not at all by pyrimidines. This complex, therefore, does not luminesce while intercalated in dsDNA, but it is bright red when conjugated to a ssDNA that is terminated by several pyrimidines. In its design, the light-switch probe incorporates a structure-switching aptamer partially hybridized to its complementary strand. The lanthanide complex is conjugated to either strand via a stable amide bond. Binding of the analyte by the structure-switching aptamer releases the complementary strand. This release precludes intercalation of the intercalator in dsDNA, which switches on its luminescence. The resulting probe turns on 21-fold upon binding to its analyte. Moreover, the structure switching aptamer is highly selective, and the long luminescence lifetime of the probe readily enables time-gating experiments for removal of the background autofluorescence of the sample.
authors.affiliation: Department of Chemistry, University of Minnesota , Minneapolis, Minnesota 55455, United States.
authors.forename: Thakshila M
authors.initials: TM
authors.lastname: Wickramaratne
authors.forename: Valerie C
authors.initials: VC
authors.lastname: Pierre
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence; D004277:DNA, Single-Stranded / Q000737:chemistry / Q000378:metabolism; D005063:Europium / Q000737:chemistry; D006801:Humans; D049449:Luminescence; D008628:Mercury / Q000097:blood / Q000378:metabolism; D008958:Models, Molecular; D009690:Nucleic Acid Conformation
chemical_list: D052157:Aptamers, Nucleotide; D004277:DNA, Single-Stranded; D005063:Europium; D008628:Mercury