title: Rapid detection of chloramphenicol residues in aquatic products using colloidal gold immunochromatographic assay.
abstract: A colloidal gold immunochromatographic assay (GICA) was developed for rapid detection of chloramphenicol (CAP) residues in aquatic products. A nitrocellulose (NC) membrane was used as the carrier, and the polyclonal CAP antibody was used as the marker protein. The average diameter of as-prepared colloidal gold nanoparticles (AuNPs) was about 20 nm. The optimal pH value of colloidal gold solutions and the amount of the antibody of CAP were 8.0 and 7.2 μg/mL, respectively. The CAP antibody was immobilized onto the conjugate pad after purification. The CAP conjugate and goat anti-rabbit IgG (secondary antibody) were coated onto the NC membrane. Next, the non-specific sites were blocked with 1% bovine serum albumin. The minimum detectable concentration of CAP in standard solution is 0.5 ng/mL, with good reproducibility. For the real samples from crucian carps injected with a single-dose of CAP in the dorsal muscles, the minimum detectable concentration of CAP residues was 0.5 µg/kg. The chromatographic analysis time was less than 10 min, and the strip had a long storage lifetime of more than 90 days at different temperatures. The strips provide a means for rapid detection of CAP residues in aquatic products.
authors.affiliation: College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China. wszcn@126.com.
authors.forename: Chennan
authors.initials: C
authors.lastname: Zhou
authors.affiliation: College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China. 21213025@zju.edu.cn.
authors.forename: Xueyin
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Shanghai Entry-Exit Inspection and Quarantine Bureau, Shanghai 200000, China. huangxinxin@shciq.gov.cn.
authors.forename: Xinxin
authors.initials: X
authors.lastname: Huang
authors.affiliation: College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China. guoxs@zju.edu.cn.
authors.forename: Xishan
authors.initials: X
authors.lastname: Guo
authors.affiliation: Yangtze Delta Region Institute of Tsinghua University, Jiaxing 310013, China. caiq@tsinghua.edu.cn.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Cai
authors.affiliation: College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China. zhusm@zju.edu.cn.
authors.forename: Songming
authors.initials: S
authors.lastname: Zhu
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis; D002347:Carps / Q000378:metabolism*; D002417:Cattle; D002701:Chloramphenicol / Q000032:analysis*; D002846:Chromatography, Affinity / Q000295:instrumentation*; D003102:Colloids / Q000737:chemistry; D004350:Drug Residues / Q000032:analysis*; D004784:Environmental Monitoring / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006046:Gold / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D011817:Rabbits
chemical_list: D000900:Anti-Bacterial Agents; D003102:Colloids; D002701:Chloramphenicol; D006046:Gold