title: Peach (Prunus persica L.).
abstract: Until now, the application of genetic transformation techniques in peach has been limited by the difficulties in developing efficient regeneration and transformation protocols. Here we describe an efficient regeneration protocol for the commercial micropropagation of GF677 rootstock (Prunus persica × Prunus amygdalus). The method is based on the production, via organogenesis, of meristematic bulk tissues characterized by a high competence for shoot regeneration. This protocol has also been used to obtain GF677 plants genetically engineered with an empty hairpin cassette (hereafter indicated as hp-pBin19), through Agrobacterium tumefaciens-mediated transformation. After 7-8 months of selection on media containing kanamycin, we obtained two genetically modified GF677 lines. PCR and Southern blot analyses were performed to confirm the genetic status.
authors.affiliation: Scienze Agrarie, Alimentari ed Ambientali D3A, Università Politecnica delle Marche, Via Brecce Bianche 10, 60131, Ancona, Italy, s.sabbadini@univpm.it.
authors.forename: Silvia
authors.initials: S
authors.lastname: Sabbadini
authors.forename: Tiziana
authors.initials: T
authors.lastname: Pandolfini
authors.forename: Luca
authors.initials: L
authors.lastname: Girolomini
authors.forename: Barbara
authors.initials: B
authors.lastname: Molesini
authors.forename: Oriano
authors.initials: O
authors.lastname: Navacchi
mesh_terms: D000064:Acclimatization; D016960:Agrobacterium tumefaciens / Q000235:genetics; D005818:Genetic Engineering / Q000379:methods*; D027861:Prunus / Q000235:genetics* / Q000254:growth & development* / Q000502:physiology; D012038:Regeneration; D014170:Transformation, Genetic