title: Aptamer photoregulation in vivo.
abstract: The in vivo application of aptamers as therapeutics could be improved by enhancing target-specific accumulation while minimizing off-target uptake. We designed a light-triggered system that permits spatiotemporal regulation of aptamer activity in vitro and in vivo. Cell binding by the aptamer was prevented by hybridizing the aptamer to a photo-labile complementary oligonucleotide. Upon irradiation at the tumor site, the aptamer was liberated, leading to prolonged intratumoral retention. The relative distribution of the aptamer to the liver and kidney was also significantly decreased, compared to that of the free aptamer.
authors.affiliation: Laboratory for Biomaterials and Drug Delivery, Department of Anesthesiology, Division of Critical Care Medicine, Children's Hospital Boston, Harvard Medical School, Boston, MA 02115; and Department of Chemical Engineering and Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA 02139.
authors.forename: Lele
authors.initials: L
authors.lastname: Li
authors.affiliation: Laboratory for Biomaterials and Drug Delivery, Department of Anesthesiology, Division of Critical Care Medicine, Children's Hospital Boston, Harvard Medical School, Boston, MA 02115; and Department of Chemical Engineering and Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA 02139.
authors.forename: Rong
authors.initials: R
authors.lastname: Tong
authors.affiliation: Laboratory for Biomaterials and Drug Delivery, Department of Anesthesiology, Division of Critical Care Medicine, Children's Hospital Boston, Harvard Medical School, Boston, MA 02115; and Department of Chemical Engineering and Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA 02139.
authors.forename: Hunghao
authors.initials: H
authors.lastname: Chu
authors.affiliation: Laboratory for Biomaterials and Drug Delivery, Department of Anesthesiology, Division of Critical Care Medicine, Children's Hospital Boston, Harvard Medical School, Boston, MA 02115; and Department of Chemical Engineering and Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA 02139.
authors.forename: Weiping
authors.initials: W
authors.lastname: Wang
authors.affiliation: Department of Chemical Engineering and Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA 02139 rlanger@mit.edu daniel.kohane@childrens.harvard.edu.
authors.forename: Robert
authors.initials: R
authors.lastname: Langer
authors.affiliation: Laboratory for Biomaterials and Drug Delivery, Department of Anesthesiology, Division of Critical Care Medicine, Children's Hospital Boston, Harvard Medical School, Boston, MA 02115; and rlanger@mit.edu daniel.kohane@childrens.harvard.edu.
authors.forename: Daniel S
authors.initials: DS
authors.lastname: Kohane
mesh_terms: D000818:Animals; D052157:Aptamers, Nucleotide / Q000235:genetics / Q000378:metabolism* / Q000493:pharmacokinetics*; D001483:Base Sequence; D002232:Carbocyanines / Q000378:metabolism; D045744:Cell Line, Tumor; D031541:Fluorescence Resonance Energy Transfer; D008325:Mammary Neoplasms, Experimental / Q000378:metabolism* / Q000473:pathology; D008819:Mice, Nude; D008856:Microscopy, Fluorescence; D009693:Nucleic Acid Hybridization / Q000528:radiation effects; D009841:Oligonucleotides / Q000235:genetics; D012867:Skin / Q000378:metabolism* / Q000473:pathology / Q000528:radiation effects; D014018:Tissue Distribution; D014466:Ultraviolet Rays
chemical_list: D052157:Aptamers, Nucleotide; D002232:Carbocyanines; D009841:Oligonucleotides; C089187:cyanine dye 3; C085321:cyanine dye 5
keywords: aptamer; cancer targeting; light triggering