title: Pertussis serosurveillance study in Izmir, Turkey.
abstract: Pertussis is a life-threatening, vaccine-preventable infection. Adults who can be asymptomatic may infect infants. The aim of this study is to determine the IgG antibody levels against pertussis toxin (PT) and filamentous hemagglutinin from 6 months to ≥60 years in Izmir, Turkey. A cluster sample design developed by Expanded Programme on Immunization of the World Health Organization was carried out for the selection of the study population, which consisted of 399 healthy subjects. In-house ELISA was studied in Turkish Public Health Institution. Antibody levels of <10 EU/ml, ≥10 EU/ml and ≥100 EU/ml were accepted as non-immune, immune and possible acute/recent infection, respectively. Anti-PT antibody levels were 8.5% <10 EU/ml, 68.2% 10-100 EU/ml and 23.3% ≥100 EU/ml; the latter was correlated with possible acute/recent infection. Results showed that pertussis is endemic, particularly among adolescents and adults, which is a threat for infants who have not completed their primary immunization.
authors.affiliation: Department of Pediatrics, Ege University.
authors.forename: Ebru
authors.initials: E
authors.lastname: Türkoglu
authors.affiliation: Microbiology Reference Laboratories, Turkish Public Health Institution.
authors.forename: Cemile
authors.initials: C
authors.lastname: Sönmez
authors.affiliation: Department of Pediatrics, Ege University.
authors.forename: Zafer
authors.initials: Z
authors.lastname: Kurugöl
authors.affiliation: Microbiology Department, Diskapı Yildirim Beyazit Training and Reserach Hospital nilaycoplu@gmail.com.
authors.forename: Nilay
authors.initials: N
authors.lastname: Çöplü
authors.affiliation: Department of Pediatrics, Ege University.
authors.forename: Guldane
authors.initials: G
authors.lastname: Koturoğlu
mesh_terms: D018829:Adhesins, Bacterial; D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000907:Antibodies, Bacterial / Q000097:blood*; D001886:Bordetella pertussis / Q000276:immunology* / Q000302:isolation & purification; D002648:Child; D002675:Child, Preschool; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006388:Hemagglutinins / Q000276:immunology; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology / Q000378:metabolism; D007223:Infant; D008297:Male; D008875:Middle Aged; D037342:Pertussis Toxin / Q000276:immunology*; D011159:Population Surveillance; D016036:Seroepidemiologic Studies; D014421:Turkey / Q000453:epidemiology; D010566:Virulence Factors, Bordetella; D014917:Whooping Cough / Q000097:blood* / Q000453:epidemiology; D055815:Young Adult
chemical_list: D018829:Adhesins, Bacterial; D000907:Antibodies, Bacterial; D006388:Hemagglutinins; D007074:Immunoglobulin G; D010566:Virulence Factors, Bordetella; C065273:filamentous hemagglutinin adhesin, Bordetella pertussis; D037342:Pertussis Toxin
keywords: Bordetella pertussis; ELISA; serosurveillance