title: [Effect of bovine lactoferrin biofilms in the formation of clinical strains of enteroaggregative E. coli].
abstract: OBJECTIVE
  To determine the effect of bovine lactoferrin (BLF) in the formation of biofilms in clinical enteroaggregative Escherichia coli (EAEC) strains and whether this effect is independent of iron.
  
  
  METHODS
  Two methods were used: (a) qualitative, by direct observation of optical microscopy, and (b) quantitative readings of the absorbance values using ELISA reader in the presence of bLf in concentrations of 0.01 mg mL and 1 mg/mL, with and without iron and no bLf (control). Analysis occurred in 122 strains of EAEC (60 strains from children with diarrhea and 62 healthy children) previously collected in a previous study of passive surveillance of diarrhea in the Southern Cone Lima. 31 strains of the same method were used for the qualitative study.
  
  
  RESULTS
  (A) Qualitative method: 31 strains were evaluated with and without iron. Without iron biofi formation was 77% (24/31) in the control group versus 58% (14/31) with bLf of 0.01 mg/mL and 4% (1/31) with 1 mg/ml. Iron biofilm formation was 90% (28/31) in the control group versus 55% (17/31) with bLf of 0.01 mg/mL and 4% (1/31) with 1 mg/mL. (B) Quantitative method: without iron absorbance measured at OD 560 nm of the control group was 0.7 ± 0.5 versus 0.4 ± 0.3 with bLf 0.01 mg/mL and 0.3 ± 0.2 with bLf of 1 mg/mL (p<0.0001). This decrease in the presence of bLf included iron.
  
  
  CONCLUSIONS
  bLf tends to decrease the formation of biofilms, showing an inhibitory effect in clinical isolates of EAEC; this effect is not iron-dependent.
authors.affiliation: Facultad de Medicina, Universidad peruana cayetano heredia, Lima, Perú
authors.forename: Cecilia
authors.initials: C
authors.lastname: Coveñas
authors.affiliation: Facultad de Medicina, Universidad peruana cayetano heredia, Lima, Perú
authors.forename: Georgette
authors.initials: G
authors.lastname: Dextre
authors.affiliation: Facultad de Medicina, Universidad peruana cayetano heredia, Lima, Perú
authors.forename: Diana
authors.initials: D
authors.lastname: Fernandez
authors.affiliation: Facultad de Medicina, Universidad peruana cayetano heredia, Lima, Perú
authors.forename: Susan
authors.initials: S
authors.lastname: Mosquito
authors.affiliation: Facultad de Medicina, Universidad peruana cayetano heredia, Lima, Perú
authors.forename: Theresa
authors.initials: T
authors.lastname: Ochoa
mesh_terms: D018441:Biofilms / Q000187:drug effects*; D003968:Diarrhea, Infantile / Q000382:microbiology*; D004926:Escherichia coli / Q000187:drug effects* / Q000502:physiology*; D006801:Humans; D007223:Infant; D007781:Lactoferrin / Q000494:pharmacology*; D010446:Peptide Fragments / Q000494:pharmacology*
chemical_list: D010446:Peptide Fragments; C579634:lactoferricin (4-9), cow; D007781:Lactoferrin