title: Molecular characterization and identification of target protein of an important vesicle trafficking gene AlRab7 from a salt excreting halophyte Aeluropus lagopoides.
abstract: The endomembrane system plays an important role during cellular adaptation of the plants with the extracellular environment. The small GTP-binding protein Rab7 located at the vacuolar membrane regulates the vesicle fusion with the vacuole and thereby helps in recycling of the molecules. This is the first report on isolation and characterization of AlRab7 gene from the halophyte plant, Aeluropus that extrudes NaCl through salt glands and grows luxuriantly throughout the year at the Gujarat coast, India. The AlRab7 encodes a protein with 206 amino acids, and a highly conserved effector-binding domain and four nucleotide-binding domains. The in silico analysis predicts the presence of the prenylation site for Rab geranylgeranyltransferase 2 and the Rab escort protein site. The C-terminal two cysteine residues in -XCC sequence are present for membrane attachment. Transcript expression of the AlRab7 gene was differentially regulated by different environmental stimuli such as dehydration, salinity, and hormone abscisic acid (ABA). The recombinant Escherichia coli cells showed improved growth in Luria Bertani medium supplemented with NaCl, KCl, mannitol, ABA, and indole-3-acetic acid. A novel Rab7 interacting partner AlRabring7 was identified by yeast two-hybrid screening.
authors.affiliation: 1 Discipline of Wasteland Research, CSIR-Central Salt and Marine Chemicals Research Institute, Bhavnagar, India .
authors.forename: Navya
authors.initials: N
authors.lastname: Rajan
authors.forename: Parinita
authors.initials: P
authors.lastname: Agarwal
authors.forename: Khantika
authors.initials: K
authors.lastname: Patel
authors.forename: Payal
authors.initials: P
authors.lastname: Sanadhya
authors.forename: Jackson
authors.initials: J
authors.lastname: Khedia
authors.forename: Pradeep Kumar
authors.initials: PK
authors.lastname: Agarwal
mesh_terms: D000040:Abscisic Acid / Q000494:pharmacology; D000595:Amino Acid Sequence; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D008969:Molecular Sequence Data; D010802:Phylogeny; D010937:Plant Growth Regulators / Q000494:pharmacology; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D006109:Poaceae / Q000235:genetics* / Q000378:metabolism; D011485:Protein Binding; D020133:Reverse Transcriptase Polymerase Chain Reaction; D055051:Salt-Tolerant Plants / Q000235:genetics* / Q000378:metabolism; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D012965:Sodium Chloride / Q000378:metabolism / Q000494:pharmacology; D022161:Transport Vesicles / Q000378:metabolism; D020798:Two-Hybrid System Techniques; D014867:Water / Q000494:pharmacology; D020691:rab GTP-Binding Proteins / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D000090802:rab7 GTP-Binding Proteins
chemical_list: D002352:Carrier Proteins; D018076:DNA, Complementary; D010937:Plant Growth Regulators; D010940:Plant Proteins; D000090802:rab7 GTP-Binding Proteins; D014867:Water; D012965:Sodium Chloride; D000040:Abscisic Acid; D020691:rab GTP-Binding Proteins