title: Differential adhesion of Streptococcus mutans to metallic brackets induced by saliva from caries-free and caries-active individuals.
abstract: OBJECTIVE
  The purpose of this study was to evaluate the influence of saliva obtained from caries-free and caries-active individuals on the adhesion rates of Streptococcus mutans to metallic brackets.
  
  
  METHODS
  The unstimulated whole saliva of four caries-free (decayed, missing, or filled surfaces = 0) volunteers and four caries-active (decayed, missing, or filled surfaces >12) patients were collected. The saliva samples from each group were mixed and clarified. Acquired pellicles were formed onto 30 metallic edgewise brackets for premolars for each saliva group. The brackets were put in contact with planktonic cells of Streptococcus mutans ATCC 25175. Adhesion rates were assessed by crystal violet retention technique.
  
  
  RESULTS
  A higher streptococcal adhesion pattern (P < 0.00001) was observed on acquired pellicles formed by saliva from caries-active donors.
  
  
  CONCLUSIONS
  The results showed that saliva from caries-active patients tends to increase the mutans adhesion to surfaces, which is a point of concern for orthodontists.
authors.affiliation: Department of Orthodontics, The Pontifical Catholic University of Paraná, Curitiba, Brazil Xenobiotics Research Unit, The Pontifical Catholic University of Paraná, Curitiba, Brazil.
authors.forename: Ademir Franco
authors.initials: AF
authors.lastname: do Rosário Junior
authors.forename: Luégya Amorin Henriques
authors.initials: LA
authors.lastname: Knop
authors.forename: Fernanda Brasil
authors.initials: FB
authors.lastname: Baboni
authors.forename: Alinne Ulbrich Mores
authors.initials: AU
authors.lastname: Rymovicz
authors.forename: Orlando Motohiro
authors.initials: OM
authors.lastname: Tanaka
authors.forename: Edvaldo Antonio Ribeiro
authors.initials: EA
authors.lastname: Rosa
mesh_terms: D000328:Adult; D001422:Bacterial Adhesion / Q000502:physiology*; D058491:Bacterial Load; D018441:Biofilms; D004396:Coloring Agents; D004245:DMF Index; D003722:Dental Alloys / Q000737:chemistry*; D003731:Dental Caries / Q000382:microbiology*; D044622:Dental Pellicle / Q000382:microbiology; D005260:Female; D005840:Gentian Violet; D006801:Humans; D008297:Male; D016910:Orthodontic Brackets / Q000382:microbiology*; D012463:Saliva / Q000382:microbiology*; D013295:Streptococcus mutans / Q000502:physiology*; D013696:Temperature; D013997:Time Factors; D055815:Young Adult
chemical_list: D004396:Coloring Agents; D003722:Dental Alloys; D005840:Gentian Violet
keywords: Streptococcus mutans; adhesion; caries experience; metallic bracket; saliva