title: RABEX-5 is upregulated and plays an oncogenic role in gastric cancer development by activating the VEGF signaling pathway.
abstract: RABEX-5, a guanine-nucleotide exchange factor (GEF) for RAB-5, is implicated in tumorigenesis and in the development of certain human cancers. Here, we report that RABEX-5 promotes tumor growth and the metastatic ability of gastric cancer cells both in vitro and in vivo. Expression of RABEX-5 is significantly higher in gastric cancer tissues and is associated with tumor size and lymph node metastasis. In addition, targeted silencing of RABEX-5 reduced gastric cancer cell proliferation and colony formation in vitro via the induction of a G0/G1 phase arrest, and stimulated gastric cancer cell apoptosis. Knockdown of RABEX-5 also inhibited wound healing, migration and the invasive abilities of gastric cancer cells. The results of in vivo animal experiments were also consistent with these in vitro findings. Silencing of RABEX-5 led to decreased expression of VEGF. These results indicate that RABEX-5 is upregulated and plays an oncogenic role in gastric cancer development by activating the VEGF signaling pathway.
authors.affiliation: Department of Clinical Oncology, The Central Hospital of Taian, Taian, Shangdong, People's Republic of China.
authors.forename: Shuang
authors.initials: S
authors.lastname: Wang
authors.affiliation: Department of Nursing, The Central Hospital of Taian, Taian, Shangdong, People's Republic of China.
authors.forename: Aixia
authors.initials: A
authors.lastname: Lu
authors.affiliation: Department of Clinical Oncology, The Central Hospital of Taian, Taian, Shangdong, People's Republic of China.
authors.forename: Xiangming
authors.initials: X
authors.lastname: Chen
authors.affiliation: Department of Clinical Oncology, The Central Hospital of Taian, Taian, Shangdong, People's Republic of China.
authors.forename: Lin
authors.initials: L
authors.lastname: Wei
authors.affiliation: Department of Radiation Oncology, The Cancer Prevention and Control Hospital of Taian, Taian, Shangdong, People's Republic of China.
authors.forename: Jiqiang
authors.initials: J
authors.lastname: Ding
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D017209:Apoptosis; D059447:Cell Cycle Checkpoints; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D005260:Female; D005753:Gastric Mucosa / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic; D020868:Gene Silencing; D020662:Guanine Nucleotide Exchange Factors / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D008297:Male; D008807:Mice, Inbred BALB C; D008875:Middle Aged; D009361:Neoplasm Invasiveness / Q000235:genetics / Q000473:pathology; D015398:Signal Transduction; D013270:Stomach / Q000473:pathology*; D013274:Stomach Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D015854:Up-Regulation; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics / Q000378:metabolism*
chemical_list: D020662:Guanine Nucleotide Exchange Factors; C108382:RABGEF1 protein, human; C510870:RabGEF1 protein, mouse; D042461:Vascular Endothelial Growth Factor A