title: Classification of ginseng berry (Panax ginseng C.A. MEYER) extract using 1H NMR spectroscopy and its inhibition of lipid accumulation in 3 T3-L1 cells.
abstract: BACKGROUND
  Panax ginseng is a famous traditional medicine in Korea for its beneficial effect on obesity, cardiac and liver associated diseases. The aim of this study was to investigate the metabolite in Panax ginseng (P. ginseng, Aralicaceae) berries depending on the ripen stages and evaluate its potential inhibition on adipocyte differentiation in 3 T3-L1 cells.
  
  
  METHODS
  Different ripening stage samples of P. ginseng berry were analyzed through global metabolite profiling by NMR spectroscopy. Lipid accumulation in the cells was analyzed by Oil Red O staining.
  
  
  RESULTS
  The PLS-DA clearly distinguished P. ginseng berry extract (PGBE) according to the partial ripe (PR), ripe(R) and fully ripe (FR) stage. Lipid accumulation of PGBE was examined by measuring triglyceride content and Oil-Red O staining. These results suggested that the FR stage of PGBE decrease in lipid accumulation during adipocyte differentiation and the amount of threonine, asparagine, fumarate, tyraine, tyrosine, and phenylalanine increased with longer ripening of ginseng berries.
  
  
  CONCLUSIONS
  Metabolite profiling of P. ginseng was identified by 1H NMR spectra. P. ginseng extract efficiently inhibits adipogenesis in 3 T3-L1 adipocytes concluded that the P. ginseng has the antiobesity properties.
authors.forename: Seung Ok
authors.initials: SO
authors.lastname: Yang
authors.forename: Hae Ran
authors.initials: HR
authors.lastname: Park
authors.forename: Eun Suk
authors.initials: ES
authors.lastname: Sohn
authors.forename: Sang Won
authors.initials: SW
authors.lastname: Lee
authors.forename: Hyung Don
authors.initials: HD
authors.lastname: Kim
authors.forename: Young Chang
authors.initials: YC
authors.lastname: Kim
authors.forename: Kee Hong
authors.initials: KH
authors.lastname: Kim
authors.forename: Sae Won
authors.initials: SW
authors.lastname: Na
authors.forename: Hyung-Kyoon
authors.initials: HK
authors.lastname: Choi
authors.forename: Mariadhas Valan
authors.initials: MV
authors.lastname: Arasu
authors.affiliation: Department of Medicinal Crop Research NIHHS RDA Eumsung, Chungbuk 369-873, Republic of Korea. kyo9128@korea.kr.
authors.forename: Young Ock
authors.initials: YO
authors.lastname: Kim
mesh_terms: D041721:3T3-L1 Cells; D017667:Adipocytes / Q000187:drug effects* / Q000378:metabolism; D050156:Adipogenesis / Q000187:drug effects*; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D005638:Fruit / Q000737:chemistry*; D050356:Lipid Metabolism / Q000187:drug effects*; D051379:Mice; D009765:Obesity / Q000378:metabolism*; D005894:Panax / Q000737:chemistry*; D010936:Plant Extracts / Q000494:pharmacology*; D066244:Proton Magnetic Resonance Spectroscopy / Q000379:methods; D056910:Republic of Korea; D014280:Triglycerides / Q000378:metabolism
chemical_list: D010936:Plant Extracts; D014280:Triglycerides