title: Assessment of Indian Rosewood (Dalbergia sissoo) Standardized Leaf Extract on Isoproterenol-Induced Myocardial Injury in Rats.
abstract: The present study was undertaken to evaluate the effect of alcoholic extract of Dalbergia sissoo leaf extract (DSE) on isoproterenol (ISP)-induced myocardial injury in rats. Evaluation of three doses (30, 100 and 300 mg/kg of body weight) of DSE was done in ISP-treated rats. ISP was used at 85 mg/kg body weight by subcutaneous route for two subsequent days to induce myocardial injury in rats. Assessment of myocardial injury was done by estimation of different cardiac injury markers like LDH, CK-MB. Serum cholesterol, LDL, HDL, triglycerides in serum, myocardial infarcted area, oxidative stress and histopathology in heart tissue were also assessed in rats. Mean arterial pressure and heart rate were recorded in all the groups. Rats pretreated with DSE (30, 100 and 300 mg/kg of body weight) showed significant (p < 0.05-0.001) improvement in the heart weight/body weight ratio, myocardial infarcted areas, heart rate and mean arterial pressure in ISP-induced myocardial injury. DSE showed significant (p < 0.05-0.001) improvement in serum LDH, CK-MB, cholesterol, LDL and triglyceride levels at all the dose levels. However, DSE pretreatment had no significant effect on serum HDL level. Pretreatment with DSE (30, 100 and 300 mg/kg body weight) showed significant (p < 0.001) reduction in MDA level in comparison with myocardial injured rats. Further, antioxidant potential was also improved in terms of improved activities of reduced glutathione, superoxide dismutase and catalase with the DSE pretreatment. Histopathology also showed significant improvement in heart tissue. The study suggests that DSE showed beneficial effect in ISP-induced myocardial injury in rats.
authors.affiliation: Division of Pharmacology and Toxicology, Indian Veterinary Research Institute, Izatnagar, Bareilly, 243122, Uttar Pradesh, India.
authors.forename: Jaya Kiran
authors.initials: JK
authors.lastname: Kasa
authors.forename: Thakur Uttam
authors.initials: TU
authors.lastname: Singh
authors.forename: Subhashree
authors.initials: S
authors.lastname: Parida
authors.forename: M Pule
authors.initials: MP
authors.lastname: Addison
authors.forename: Sajad Ahmad
authors.initials: SA
authors.lastname: Darzi
authors.forename: Soumen
authors.initials: S
authors.lastname: Choudhury
authors.forename: Kannan
authors.initials: K
authors.lastname: Kandasamy
authors.forename: Vishakha
authors.initials: V
authors.lastname: Singh
authors.forename: Jeevan Ranjan
authors.initials: JR
authors.lastname: Dash
authors.forename: Karuna
authors.initials: K
authors.lastname: Shanker
authors.forename: Santosh Kumar
authors.initials: SK
authors.lastname: Mishra
mesh_terms: D000818:Animals; D009202:Cardiomyopathies / Q000139:chemically induced* / Q000188:drug therapy* / Q000378:metabolism; D002316:Cardiotonic Agents / Q000633:toxicity; D035782:Dalbergia; D007545:Isoproterenol / Q000633:toxicity*; D008297:Male; D009206:Myocardium / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects / Q000502:physiology; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*; D018515:Plant Leaves; D051381:Rats; D017208:Rats, Wistar
chemical_list: D002316:Cardiotonic Agents; D010936:Plant Extracts; D007545:Isoproterenol