title: RNA-controlled assembly of tobacco mosaic virus-derived complex structures: from nanoboomerangs to tetrapods.
abstract: The in vitro assembly of artificial nanotubular nucleoprotein shapes based on tobacco mosaic virus-(TMV-)-derived building blocks yielded different spatial organizations of viral coat protein subunits on genetically engineered RNA molecules, containing two or multiple TMV origins of assembly (OAs). The growth of kinked nanoboomerangs as well as of branched multipods was determined by the encapsidated RNAs. A largely simultaneous initiation at two origins and subsequent bidirectional tube elongation could be visualized by transmission electron microscopy of intermediates and final products. Collision of the nascent tubes' ends produced angular particles with well-defined arm lengths. RNAs with three to five OAs generated branched multipods with a maximum of four arms. The potential of such an RNA-directed self-assembly of uncommon nanotubular architectures for the fabrication of complex multivalent nanotemplates used in functional hybrid materials is discussed.
authors.affiliation: Department of Molecular Biology and Plant Virology, Institute of Biomaterials and Biomolecular Systems, University of Stuttgart, Pfaffenwaldring 57, D-70569 Stuttgart, Germany. christina.wege@bio.uni-stuttgart.de.
authors.forename: Fabian J
authors.initials: FJ
authors.lastname: Eber
authors.forename: Sabine
authors.initials: S
authors.lastname: Eiben
authors.forename: Holger
authors.initials: H
authors.lastname: Jeske
authors.forename: Christina
authors.initials: C
authors.lastname: Wege
mesh_terms: D040761:Biomimetic Materials / Q000138:chemical synthesis; D003460:Crystallization / Q000379:methods; D005818:Genetic Engineering / Q000379:methods; D008422:Materials Testing; D054802:Molecular Imprinting / Q000379:methods; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure*; D010316:Particle Size; D012313:RNA / Q000737:chemistry* / Q000648:ultrastructure*; D014027:Tobacco Mosaic Virus / Q000235:genetics* / Q000648:ultrastructure*
chemical_list: D012313:RNA