title: Super-resolution microscopy approaches for live cell imaging.
abstract: By delivering optical images with spatial resolutions below the diffraction limit, several super-resolution fluorescence microscopy techniques opened new opportunities to study biological structures with details approaching molecular structure sizes. They have now become methods of choice for imaging proteins and their nanoscale dynamic organizations in live cells. In this mini-review, we describe and compare the main far-field super-resolution approaches that allow studying endogenous or overexpressed proteins in live cells.
authors.affiliation: University of Bordeaux, LP2N, UMR 5298, Talence, France; Institut d'Optique Graduate School and Centre National de la Recherche Scientifique, LP2N, UMR 5298, Talence, France.
authors.forename: Antoine G
authors.initials: AG
authors.lastname: Godin
authors.affiliation: University of Bordeaux, LP2N, UMR 5298, Talence, France; Institut d'Optique Graduate School and Centre National de la Recherche Scientifique, LP2N, UMR 5298, Talence, France.
authors.forename: Brahim
authors.initials: B
authors.lastname: Lounis
authors.affiliation: University of Bordeaux, LP2N, UMR 5298, Talence, France; Institut d'Optique Graduate School and Centre National de la Recherche Scientifique, LP2N, UMR 5298, Talence, France. Electronic address: laurent.cognet@u-bordeaux.fr.
authors.forename: Laurent
authors.initials: L
authors.lastname: Cognet
mesh_terms: D021621:Imaging, Three-Dimensional / Q000379:methods*; D057230:Limit of Detection; D008856:Microscopy, Fluorescence / Q000379:methods; D059010:Single-Cell Analysis / Q000379:methods