title: 3D-technology of the formation and maintenance of single dormant microspheres from 2000 human somatic cells and their reactivation in vitro.
abstract: We developed an original reproducible 3D-technology for preparation of single dormant microspheres consisting of 2000 somatic cells. The dynamics of microsphere assembly from mesenchymal and epithelial cells of retinal pigment epithelium was traced using time-lapse microscopy: formation of a loose aggregate over 24 h followed by its gradual consolidation and formation of a compact viable microsphere with a diameter of 100-150 μ by day 7. The cell number in the formed microspheres remains unchanged. Reactivation observed upon fusion of epithelial and/or mesenchymal microspheres results in the formation of a united compact microtissue. The fusion dynamics reproduces spherogenesis irrespective of the initial amount of co-cultured microspheres. Reactivation via two-step induced angiogenesis opens new prospects for production of vascularized microspheres and microtissues.
authors.affiliation: Research Institute of General Pathology and Pathophysiology, Russian Academy of Medical Sciences, Moscow, Russia.
authors.forename: V S
authors.initials: VS
authors.lastname: Repin
authors.forename: I N
authors.initials: IN
authors.lastname: Saburina
authors.forename: N V
authors.initials: NV
authors.lastname: Kosheleva
authors.forename: A A
authors.initials: AA
authors.lastname: Gorkun
authors.forename: I M
authors.initials: IM
authors.lastname: Zurina
authors.forename: A A
authors.initials: AA
authors.lastname: Kubatiev
mesh_terms: D015703:Antigens, CD / Q000378:metabolism; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D058750:Epithelial-Mesenchymal Transition; D006801:Humans; D055213:Retinal Pigment Epithelium / Q000166:cytology; D018874:Spheroids, Cellular / Q000502:physiology*; D059008:Time-Lapse Imaging; D042461:Vascular Endothelial Growth Factor A / Q000502:physiology
chemical_list: D015703:Antigens, CD; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A