title: Anticancer Properties of Novel Rhenium Pentylcarbanato Compounds against MDA-MB-468(HTB-132) Triple Node Negative Human Breast Cancer Cell Lines.
abstract: OBJECTIVE
  To study the efficacy of novel rhenium compounds to treat triple node negative breast cancer.
  
  
  METHODS
  Six (6) novel rhenium pentycarbanato compounds (PC1-6) were synthesized and triple node negative breast cancer cell lines HTB-132 and Balb/c mouse kidney cell lines were treated with each of them for 48 hours. The results were analyzed by a common trypan blue cell death assay system and statistically analyzed.
  
  
  METHODS
  The compounds were synthesized, analyzed and evaluated at the Department of Chemistryof Morgan State University, Baltimore, Maryland and the Pharmaceutical Sciences Department of Elizabeth City State University campus of the University of North Carolina system.
  
  
  METHODS
  The novel rhenium compounds were synthesized from one-pot reactions of Re2(CO)10with the corresponding α-diimine ligands in 1-pentanol.The compounds were characterized spectroscopically. The cell lines were cultured by standard cell culture procedure and treated with each of the six compounds in DMSO for 48 hours with a negative control and a DMSO vehicular control along with a cisplatin positive control.The cytotoxicity was evaluated by standard trypan blue assay and the results were statistically analyzed.
  
  
  RESULTS
  The trypan blueassay reveals that these compounds have significant cytotoxicity against MDA-MB-468 (HTB-132) triple node negative breast cancer cell lines and are less nephrotoxic than cisplatin.
  
  
  CONCLUSIONS
  The novel rhenium compoundsPC 1-6can potentially find applications in the treatment of highly malignant triple node negative breast cancer.
authors.affiliation: Department of Biology and Pharmaceutical Sciences, Elizabeth City State University, University of North Carolina, Elizabeth City, NC, 27909, USA.
authors.forename: Carl
authors.initials: C
authors.lastname: Parson
authors.affiliation: Department of Biology and Pharmaceutical Sciences, Elizabeth City State University, University of North Carolina, Elizabeth City, NC, 27909, USA.
authors.forename: Valerie
authors.initials: V
authors.lastname: Smith
authors.affiliation: Department of Biology and Pharmaceutical Sciences, Elizabeth City State University, University of North Carolina, Elizabeth City, NC, 27909, USA.
authors.forename: Christopher
authors.initials: C
authors.lastname: Krauss
authors.affiliation: Department of Biology and Pharmaceutical Sciences, Elizabeth City State University, University of North Carolina, Elizabeth City, NC, 27909, USA.
authors.forename: Hirendra N
authors.initials: HN
authors.lastname: Banerjee
authors.affiliation: Department of Biomedical Sciences and Pathobiology, Virginia-Maryland Regional College of Veterinary Medicine, Virginia Polytechnic Institute and State University, Blacksburg, Virginia, 24061, USA.
authors.forename: Christopher
authors.initials: C
authors.lastname: Reilly
authors.affiliation: Department of Chemistry, University of Cincinnati, Cincinnati, OH, 45221, USA.
authors.forename: Jeanette A
authors.initials: JA
authors.lastname: Krause
authors.affiliation: Department of Biology, Morgan State University, Baltimore, MD, 21251, USA.
authors.forename: James M
authors.initials: JM
authors.lastname: Wachira
authors.affiliation: Department of Chemistry, Morgan State University, Baltimore, MD, 21251, USA.
authors.forename: Dipak
authors.initials: D
authors.lastname: Giri
authors.affiliation: Department of Chemistry, Morgan State University, Baltimore, MD, 21251, USA.
authors.forename: Angela
authors.initials: A
authors.lastname: Winstead
authors.affiliation: Department of Chemistry, Morgan State University, Baltimore, MD, 21251, USA.
authors.forename: Santosh K
authors.initials: SK
authors.lastname: Mandal
keywords: HTB-132 breast cancer cells; Rhenium pentylcarbonato compounds; cytotoxicity; trypan blue assay