title: Effects of temperature and fish oil supplementation on ovarian development and foxl2 mRNA expression in spotted scat Scatophagus argus.
abstract: In this study, the complete foxl2 complementary (c)DNA sequence was isolated by simple modular-architecture research tool (SMART)er rapid amplification of cDNA ends (RACE). Two year-old female spotted scat, Scatophagus argus, were reared at different temperatures (23, 26 and 29° C) for 6 weeks, or fed with different concentrations of dietary fish oil (0, 2 or 6%) for 8 weeks. Ovarian development, serum oestradiol-17β (E2 ) levels, as well as ovarian foxl2 expression were measured. At the end of experiment, ovarian foxl2 messenger (m)RNA expression in fish reared at 23 and 26° C was significantly higher than that in fish reared at 29° C, and that in 2 and 6% fish oil groups was also significantly higher than that in control group (P < 0·05). Serum E2 levels exhibited the same trend with foxl2 mRNA expression in temperature treatment groups and fish oil fed groups. There was a significant positive correlation between stage of oocytes and foxl2 expressions. Results showed that from 23 to 29° C, the optimal temperature for ovarian development in S. argus was 23-26° C, and 6% fish oil supplementation could effectively promote ovarian development. Optimal temperature and fish oil supplement might increase ovarian foxl2 mRNA expressions to promote ovarian development in S. argus.
authors.affiliation: Fisheries College, Guangdong Ocean University, Zhanjiang, 524088, China; Key Laboratory of Aquaculture in South China Sea for Aquatic Economic Animal of Guangdong Higher Education Institutes, Guangdong Ocean University, Zhanjiang, 524088, China.
authors.forename: G-L
authors.initials: GL
authors.lastname: Li
authors.forename: M-Z
authors.initials: MZ
authors.lastname: Zhang
authors.forename: S-P
authors.initials: SP
authors.lastname: Deng
authors.forename: H-P
authors.initials: HP
authors.lastname: Chen
authors.forename: C-H
authors.initials: CH
authors.lastname: Zhu
mesh_terms: D000818:Animals; D019587:Dietary Supplements; D004958:Estradiol / Q000097:blood; D005260:Female; D005395:Fish Oils; D029941:Fish Proteins / Q000235:genetics; D051858:Forkhead Transcription Factors / Q000235:genetics*; D018507:Gene Expression Regulation, Developmental; D009865:Oocytes / Q000254:growth & development; D010053:Ovary / Q000254:growth & development*; D010473:Perciformes / Q000235:genetics / Q000254:growth & development*; D012333:RNA, Messenger / Q000235:genetics; D012733:Sex Differentiation; D013696:Temperature
chemical_list: D005395:Fish Oils; D029941:Fish Proteins; D051858:Forkhead Transcription Factors; D012333:RNA, Messenger; D004958:Estradiol
keywords: environmental factors; gene expression; oestradiol-17β; sex steroid hormones