title: Ability of bacteriophage in resolving wound infection caused by multidrug-resistant Acinetobacter baumannii in uncontrolled diabetic rats.
abstract: Acinetobacter baumannii, a substantial nosocomial pathogen, has developed resistance to almost all available antimicrobial drugs. Bacteriophage therapy is a possible alternative treatment for multidrug-resistant (MDR) bacterial infections. In this study, we have successfully isolated bacteriophage active against clinical strains of A. baumannii by enrichment from hospital sewage sludge using representatives of those strains. The bacteriophage isolated against A. baumannii formed plaques against beta-lactamases producing strains of A. baumannii. The utility of bacteriophage specific for A. baumannii to resolve wound infection in uncontrolled diabetic rats was evaluated. Five groups of uncontrolled diabetic rats were used. Group I was noninfected (Control), Group II was infected with MDR A. baumannii and challenged with bacteriophage, Group III was infected with MDR A. baumannii, Group IV was infected with MDR A. baumannii and challenged with antibiotic colistin, and Group V consisted of noninfected rats and sprayed with phage (Phage control). A significant decrease in infection, period of epithelization, and wound contraction was observed in the phage-challenged group when compared with antibiotic-treated uncontrolled diabetic rats and the control group. To conclude the study, new insights are provided into the biology of the broad host range of A. baumannii phage, demonstrating that A. baumannii phage has prospects for the treatment of infections caused by the MDR A. baumannii.
authors.affiliation: 1 Department of Microbiology, S.S. Institute of Medical Sciences and Research Centre , Davangere, Karnataka, India .
authors.forename: VinodKumar Chickmangalure
authors.initials: VC
authors.lastname: Shivaswamy
authors.forename: Suneeta Basavaraj
authors.initials: SB
authors.lastname: Kalasuramath
authors.forename: Chethan Kumar
authors.initials: CK
authors.lastname: Sadanand
authors.forename: Abhishek Kilagere
authors.initials: AK
authors.lastname: Basavaraju
authors.forename: Varsha
authors.initials: V
authors.lastname: Ginnavaram
authors.forename: Sumanth
authors.initials: S
authors.lastname: Bille
authors.forename: Sanjay Saju
authors.initials: SS
authors.lastname: Ukken
authors.forename: Usha Nandini
authors.initials: UN
authors.lastname: Pushparaj
mesh_terms: D000151:Acinetobacter Infections / Q000382:microbiology / Q000628:therapy* / Q000821:virology*; D040981:Acinetobacter baumannii / Q000187:drug effects / Q000378:metabolism / Q000821:virology*; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001435:Bacteriophages / Q000378:metabolism*; D003091:Colistin / Q000494:pharmacology; D003428:Cross Infection / Q000382:microbiology / Q000628:therapy / Q000821:virology; D003921:Diabetes Mellitus, Experimental / Q000382:microbiology / Q000821:virology*; D024901:Drug Resistance, Multiple, Bacterial / Q000187:drug effects; D008297:Male; D008826:Microbial Sensitivity Tests / Q000379:methods; D051381:Rats; D017208:Rats, Wistar; D014946:Wound Infection / Q000382:microbiology / Q000628:therapy* / Q000821:virology*; D001618:beta-Lactamases / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D001618:beta-Lactamases; D003091:Colistin