title: Gene delivery efficiency and intracellular trafficking of novel poly(allylamine) derivatives.
abstract: Non-viral gene carriers for safe and efficient gene transfection have become of particular interest among researchers of different disciplines ranging from physical chemistry to biotechnology. Recently polymeric vectors have been extensively studied as potentially new gene transfer agents. Until now most of the research efforts were made to optimize the gene-to-polymer weight ratio of polyplexes for safe and efficient gene transfection. In this work, we report on the development of novel poly(allylamine) derivatives with different balance of the primary, secondary, tertiary, and quaternary amino groups. All derivatives were able to complex pDNA into polyplexes at low gene-to-polymer weight ratios i.e., 1:1 or 1:2. Moreover, the examined polyplexes were less cytotoxic and showed better transfection efficiency when compared to linear poly(ethyleneimine). These results indicate that the presence of quaternary ammonium groups is important in the formation of stable polyplexes. Polymers with all types of amino groups showed large potential for gene delivery. Furthermore, polyplexes with such derivatives were well internalized by cells and ended up into acidic late endosomes.
authors.affiliation: Department of Physical Chemistry and Electrochemistry, Faculty of Chemistry, Jagiellonian University, Ingardena 3, 30-060 Kraków, Poland; Academic Centre for Materials and Nanotechnology, AGH University of Science and Technology, al. Mickiewicza 30, 30-059 Krakow, Poland. Electronic address: wytrwal@chemia.uj.edu.pl.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Wytrwal
authors.affiliation: Centre de Biophysique Moléculaire, UPR4301 CNRS, Rue Charles Sadron, 45071 Orléans Cedex 2, France.
authors.forename: Chloe
authors.initials: C
authors.lastname: Leduc
authors.affiliation: Department of Biophysics, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Gronostajowa 7, 30-387 Kraków, Poland; Laboratory of Imaging and Atomic Force Spectroscopy, Malopolska Centre of Biotechnology, Jagiellonian University, Gronostajowa 7A, 30-387 Kraków, Poland.
authors.forename: Michal
authors.initials: M
authors.lastname: Sarna
authors.affiliation: Centre de Biophysique Moléculaire, UPR4301 CNRS, Rue Charles Sadron, 45071 Orléans Cedex 2, France.
authors.forename: Cristine
authors.initials: C
authors.lastname: Goncalves
authors.affiliation: Department of Physical Chemistry and Electrochemistry, Faculty of Chemistry, Jagiellonian University, Ingardena 3, 30-060 Kraków, Poland.
authors.forename: Mariusz
authors.initials: M
authors.lastname: Kepczynski
authors.affiliation: Centre de Biophysique Moléculaire, UPR4301 CNRS, Rue Charles Sadron, 45071 Orléans Cedex 2, France.
authors.forename: Patrick
authors.initials: P
authors.lastname: Midoux
authors.affiliation: Department of Physical Chemistry and Electrochemistry, Faculty of Chemistry, Jagiellonian University, Ingardena 3, 30-060 Kraków, Poland.
authors.forename: Maria
authors.initials: M
authors.lastname: Nowakowska
authors.affiliation: Centre de Biophysique Moléculaire, UPR4301 CNRS, Rue Charles Sadron, 45071 Orléans Cedex 2, France. Electronic address: chantal.pichon@cnrs-orleans.fr.
authors.forename: Chantal
authors.initials: C
authors.lastname: Pichon
mesh_terms: D001692:Biological Transport; D002470:Cell Survival / Q000187:drug effects; D004247:DNA / Q000008:administration & dosage* / Q000737:chemistry; D011992:Endosomes / Q000378:metabolism; D018014:Gene Transfer Techniques; D006367:HeLa Cells; D006801:Humans; D008156:Luciferases / Q000235:genetics / Q000378:metabolism; D010957:Plasmids; D011073:Polyamines / Q000008:administration & dosage* / Q000737:chemistry
chemical_list: D011073:Polyamines; C063994:polyallylamine; D004247:DNA; D008156:Luciferases
keywords: Gene delivery; Nanocondensation; Non-viral gene vectors; Poly(allylamine); Transfection efficiency