title: [Effects of receptor-interacting protein 140 gene over-expression on the migration and proliferation of neuroblastoma cells].
abstract: OBJECTIVE
  To explore the effects of receptor interacting protein (RIP)140 gene over-expression upon the migration and proliferation of neuroblastoma cells in vitro.
  
  
  METHODS
  The N2a RIP140 over-expression model (N2a-rip140) was constructed by lipofection and validated by real-time polymerase chain reaction (PCR) and Western blot. The migration and proliferation capabilities were compared between N2a-rip140 and its parents by Transwell chamber and CCK-8.
  
  
  RESULTS
  The N2a RIP140 over-expression model (N2a-rip140) was successfully constructed. Compared to N2a group, the RIP140 mRNA and protein expression levels of N2a-rip140 were remarkably up-regulated. Transwell assay showed that the over-expression of RIP140 inhibited N2a cell migration ((70 ± 20) vs (5 ± 4) cells, P < 0.05). CCK-8 assay showed that the proliferation rates of N2a group at 24, 48, 72 h were 1.567 ± 0.107, 3.018 ± 0.212 and 4.112 ± 0.221 respectively. And those of N2a-rip140 group were 1.561 ± 0.281, 2.232 ± 0.235 and 4.025 ± 0.217 respectively. No significant difference existed in proliferation rates at different timepoints among N2a, N2a-M and N2a-rip140 groups (all P > 0.05).
  
  
  CONCLUSIONS
  RIP140 over-expression effectively inhibits N2a cell migration. However it has no significant effect on the proliferation of N2a cells.
authors.affiliation: Department of Pediatrics, Peking University People's Hospital, Beijing 100044, China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Feng
authors.forename: Weidong
authors.initials: W
authors.lastname: Yu
authors.forename: Rong
authors.initials: R
authors.lastname: Liang
authors.forename: Cheng
authors.initials: C
authors.lastname: Shi
authors.forename: Zhuran
authors.initials: Z
authors.lastname: Zhao
authors.affiliation: Email: gjzhxx@sina.com.
authors.forename: Jingzhu
authors.initials: J
authors.lastname: Guo
mesh_terms: D048868:Adaptor Proteins, Signal Transducing; D000818:Animals; D015153:Blotting, Western; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D015870:Gene Expression; D051379:Mice; D009447:Neuroblastoma; D009687:Nuclear Proteins; D000078723:Nuclear Receptor Interacting Protein 1; D012333:RNA, Messenger; D060888:Real-Time Polymerase Chain Reaction; D015854:Up-Regulation
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D009687:Nuclear Proteins; D000078723:Nuclear Receptor Interacting Protein 1; D012333:RNA, Messenger