title: Michaelis-Menten kinetics under non-isothermal conditions.
abstract: We extend the celebrated Michaelis-Menten kinetics description of an enzymatic reaction taking into consideration the presence of a thermal driving force. A coupling of chemical and thermal driving forces is expected from the principle of non-equilibrium thermodynamics, and specifically we obtain an additional term to the classical Michaelis-Menten kinetic equation, which describes the coupling in terms of a single parameter. A companion equation for the heat flux is also derived, which actually can exist even in the absence of a temperature difference. Being thermodynamic in nature, this result is general and independent of the detailed mechanism of the coupling. Conditions for the experimental verification of the new equation are discussed.
authors.affiliation: Department of Chemistry, Norwegian University of Science and Technology, Trondheim, Norway. anders.lervik@ntnu.no signe.kjelstrup@ntnu.no.
authors.forename: Anders
authors.initials: A
authors.lastname: Lervik
authors.forename: Signe
authors.initials: S
authors.lastname: Kjelstrup
authors.forename: Hong
authors.initials: H
authors.lastname: Qian
mesh_terms: D055162:Biocatalysis; D004798:Enzymes / Q000737:chemistry* / Q000378:metabolism*; D007700:Kinetics; D008956:Models, Chemical; D013696:Temperature; D013816:Thermodynamics
chemical_list: D004798:Enzymes