title: Generation of Induced Pluripotent Stem Cells in Rabbits.
abstract: We describe a procedure for generating induced pluripotent stem cell lines in rabbits, using retroviral vectors expressing Oct4, Sox2, Klf4, and c-Myc of human origin to reprogram rabbit fibroblasts prepared from an ear skin biopsy. We also provide detailed procedures for characterizing the resulting iPSC lines, including the analysis of pluripotency marker expression by RT-qPCR, immunolabeling, and fluorescent-associated cell sorting, the evaluation of pluripotency by teratoma production and genetic stability by karyotyping.
authors.affiliation: INSERM U846, Bron, 69500, France. marielle.afanassieff@inserm.fr.|Stem Cell and Brain Research Institute, Bron, 69500, France. marielle.afanassieff@inserm.fr.|Université de Lyon, Villeurbanne, 69100, France. marielle.afanassieff@inserm.fr.
authors.forename: Marielle
authors.initials: M
authors.lastname: Afanassieff
authors.affiliation: INSERM U846, Bron, 69500, France.|Stem Cell and Brain Research Institute, Bron, 69500, France.|Université de Lyon, Villeurbanne, 69100, France.
authors.forename: Yann
authors.initials: Y
authors.lastname: Tapponnier
authors.affiliation: INSERM U846, Bron, 69500, France.|Stem Cell and Brain Research Institute, Bron, 69500, France.|Université de Lyon, Villeurbanne, 69100, France.
authors.forename: Pierre
authors.initials: P
authors.lastname: Savatier
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods; D002454:Cell Differentiation; D002478:Cells, Cultured; D065150:Cellular Reprogramming; D000067470:Cellular Reprogramming Techniques / Q000379:methods; D002678:Chimera / Q000235:genetics; D004431:Ear, External / Q000166:cytology; D005347:Fibroblasts / Q000166:cytology; D015870:Gene Expression; D005822:Genetic Vectors / Q000235:genetics; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology* / Q000637:transplantation; D007621:Karyotyping; D000090062:Kruppel-Like Factor 4; D051741:Kruppel-Like Transcription Factors / Q000235:genetics / Q000502:physiology; D016513:Mice, SCID; D009028:Morula / Q000166:cytology; D050814:Octamer Transcription Factor-3 / Q000235:genetics / Q000502:physiology; D016271:Proto-Oncogene Proteins c-myc / Q000235:genetics / Q000502:physiology; D011817:Rabbits; D011994:Recombinant Proteins / Q000235:genetics; D012190:Retroviridae / Q000235:genetics; D055748:SOXB1 Transcription Factors / Q000235:genetics / Q000502:physiology; D013724:Teratoma / Q000473:pathology; D019076:Transgenes
chemical_list: C000715467:KLF4 protein, human; C000715468:Klf4 protein, mouse; D000090062:Kruppel-Like Factor 4; D051741:Kruppel-Like Transcription Factors; C489427:MYC protein, human; D050814:Octamer Transcription Factor-3; C494173:POU5F1 protein, human; D016271:Proto-Oncogene Proteins c-myc; D011994:Recombinant Proteins; C527782:SOX2 protein, human; D055748:SOXB1 Transcription Factors
keywords: Induced pluripotent stem cells; Klf4; Oct4; Pluripotency genes; Rabbit; Reprogramming; Retroviral vectors; Skin fibroblasts; Sox2; c-Myc