title: Modified gold surfaces by 6-(ferrocenyl)hexanethiol/dendrimer/gold nanoparticles as a platform for the mediated biosensing applications.
abstract: An electrochemical biosensor mediated by using 6-(Ferrocenyl) hexanethiol (FcSH) was fabricated by construction of gold nanoparticles (AuNPs) on the surface of polyamidoamine dendrimer (PAMAM) modified gold electrode. Glucose oxidase (GOx) was used as a model enzyme and was immobilized onto the gold surface forming a self assembled monolayer via FcSH and cysteamine. Cyclic voltammetry and amperometry were used for the characterization of electrochemical response towards glucose substrate. Following the optimization of medium pH, enzyme loading, AuNP and FcSH amount, the linear range for the glucose was studied and found as 1.0 to 5.0mM with the detection limit (LOD) of 0.6mM according to S/N=3. Finally, the proposed Au/AuNP/(FcSH+Cyst)/PAMAM/GOx biosensor was successfully applied for the glucose analysis in beverages, and the results were compared with those obtained by HPLC.
authors.affiliation: Ege University, Faculty of Science, Biochemistry Department, 35100 Bornova-Izmir, Turkey.
authors.forename: Murat
authors.initials: M
authors.lastname: Karadag
authors.affiliation: Ege University, Faculty of Science, Biochemistry Department, 35100 Bornova-Izmir, Turkey.
authors.forename: Caner
authors.initials: C
authors.lastname: Geyik
authors.affiliation: Ege University, Faculty of Science, Biochemistry Department, 35100 Bornova-Izmir, Turkey.
authors.forename: Dilek Odaci
authors.initials: DO
authors.lastname: Demirkol
authors.affiliation: Ege University, Faculty of Science, Chemistry Department, 35100, Bornova-Izmir, Turkey.
authors.forename: F Nil
authors.initials: FN
authors.lastname: Ertas
authors.affiliation: Ege University, Faculty of Science, Biochemistry Department, 35100 Bornova-Izmir, Turkey. Electronic address: suna.timur@ege.edu.tr.
authors.forename: Suna
authors.initials: S
authors.lastname: Timur
mesh_terms: D001628:Beverages / Q000032:analysis; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D001786:Blood Glucose / Q000032:analysis; D050091:Dendrimers / Q000737:chemistry*; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism; D005296:Ferrous Compounds / Q000737:chemistry*; D005947:Glucose / Q000032:analysis; D005949:Glucose Oxidase / Q000737:chemistry / Q000378:metabolism; D006046:Gold / Q000737:chemistry*; D006801:Humans; D057230:Limit of Detection; D053768:Metal Nanoparticles / Q000737:chemistry*; D018625:Microscopy, Atomic Force
chemical_list: D001786:Blood Glucose; D050091:Dendrimers; D004800:Enzymes, Immobilized; D005296:Ferrous Compounds; C104700:PAMAM Starburst; D006046:Gold; D005949:Glucose Oxidase; D005947:Glucose