title: Effects of ephrinB2 gene siRNA on the biological behavior of human colorectal cancer cells.
abstract: Colorectal cancer (CRC) is a common gastrointestinal malignancy worldwide and is a lethal and aggressive malignancy with a dismal prognosis. EphrinB2 is a membrane-bound ligand and has an intracellular domain that also possesses an intrinsic signaling capacity called 'reverse signaling'. In the present study, CRC cell lines were screened for high expression of ephrinB2. Small interfering RNA (siRNA) knockdown of ephrinB2 was performed in human SW480 CRC cells. The levels of expression of ephrinB2, VEGF, CD105 and matrix metalloproteinase 9 (MMP9) protein were measured by western blotting, and messenger RNA (mRNA) levels were measured using real-time PCR. Apoptosis and cell cycle distribution were determined using flow cytometry. Cell proliferation was measured by a methyl thiazole tetrazolium (MTT) test and a scratch healing experiment was used to measure the extent of cell migration. A Transwell assay was used to detect the extent of cell invasion. The results showed that RNA interference (RNAi) of ephrinB2 effectively silenced the ephrinB2 gene at both the mRNA and protein levels in SW480 cells and inhibited the proliferation, invasion, migration and angiogenesis and induced apoptosis in SW480 cells. These effects may be attributed to VEGF and MMP9 regulation.
authors.affiliation: Department of General Surgery, First Affiliated Hospital, Chongqing Medical University, Chongqing 400010, P.R. China.
authors.forename: Peiwu
authors.initials: P
authors.lastname: Li
authors.affiliation: Department of General Surgery, First Affiliated Hospital, Chongqing Medical University, Chongqing 400010, P.R. China.
authors.forename: Wangsheng
authors.initials: W
authors.lastname: Chen
authors.affiliation: Department of Emergency, Lanzhou University Second Hospital, Lanzhou 730000, P.R. China.
authors.forename: Yingzhen
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Emergency, Lanzhou University Second Hospital, Lanzhou 730000, P.R. China.
authors.forename: Xu
authors.initials: X
authors.lastname: Fu
authors.affiliation: Department of General Surgery, First Affiliated Hospital, Chongqing Medical University, Chongqing 400010, P.R. China.
authors.forename: Kunming
authors.initials: K
authors.lastname: Wen
authors.affiliation: Department of General Surgery, First Affiliated Hospital, Chongqing Medical University, Chongqing 400010, P.R. China.
authors.forename: Jiang
authors.initials: J
authors.lastname: Qian
authors.affiliation: Department of General Surgery, First Affiliated Hospital, Chongqing Medical University, Chongqing 400010, P.R. China.
authors.forename: Chun
authors.initials: C
authors.lastname: Huang
authors.affiliation: Department of General Surgery, First Affiliated Hospital, Chongqing Medical University, Chongqing 400010, P.R. China.
authors.forename: Zhongxue
authors.initials: Z
authors.lastname: Fu
mesh_terms: D015703:Antigens, CD / Q000235:genetics / Q000378:metabolism; D017209:Apoptosis; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015179:Colorectal Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D000071063:Endoglin; D036388:Ephrin-B2 / Q000235:genetics* / Q000378:metabolism*; D015972:Gene Expression Regulation, Neoplastic; D055785:Gene Knockdown Techniques; D057809:HEK293 Cells; D019073:HT29 Cells; D006801:Humans; D020780:Matrix Metalloproteinase 9 / Q000235:genetics / Q000378:metabolism; D034741:RNA, Small Interfering / Q000378:metabolism*; D011956:Receptors, Cell Surface / Q000235:genetics / Q000378:metabolism; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics / Q000378:metabolism
chemical_list: D015703:Antigens, CD; C064206:ENG protein, human; D000071063:Endoglin; D036388:Ephrin-B2; D034741:RNA, Small Interfering; D011956:Receptors, Cell Surface; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A; C579270:MMP9 protein, human; D020780:Matrix Metalloproteinase 9