title: Neuroprotective Effects of β-Asarone Against 6-Hydroxy Dopamine-Induced Parkinsonism via JNK/Bcl-2/Beclin-1 Pathway.
abstract: β-asarone, a major component of Acorus tatarinowii Schott, has positive effects in neurodegeneration disease, however, its effect on the Parkinson's disease (PD) remains unclear. In this study, the effects of β-asarone on behavioral tests, neurotransmitters, tyrosine hydroxylase (TH), and α-synuclein (α-syn) were investigated in 6-hydroxydopamine (6-OHDA) induced rats. Furthermore, the JNK/Bcl-2/Beclin-1 autophagy pathway was also studied. The results showed that β-asarone improved the behavioral symptoms of rats in the open field, rotarod test, initiation time, and stepping time. And it increased the HVA, Dopacl, and 5-HIAA levels in striatum but not the DA and 5-HT levels. After administration of β-asarone, the TH level was elevated but the α-syn was declined in rats. It inhibited the expressions of LC3-II, but increased the p62 expression in SN4741 cells. Moreover, it affected the expressions of Beclin-1, Bcl-2, JNK, and p-JNK in vivo. We deduced that β-asarone may firstly downregulate expressions of JNK and p-JNK, and then indirectly increase the expression of Bcl-2. And the function of Beclin-1 could be inhibited, which could inhibit autophagy activation. Collectively, all data indicated that β-asarone may be explored as a potential therapeutic agent in PD therapy.
authors.affiliation: The First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, 510405, China.
authors.forename: Sheng
authors.initials: S
authors.lastname: Zhang
authors.affiliation: The First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, 510405, China.
authors.forename: Xue-Hong
authors.initials: XH
authors.lastname: Gui
authors.affiliation: The First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, 510405, China.
authors.forename: Li-Ping
authors.initials: LP
authors.lastname: Huang
authors.affiliation: The First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, 510405, China.
authors.forename: Min-Zhen
authors.initials: MZ
authors.lastname: Deng
authors.affiliation: The First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, 510405, China.
authors.forename: Ruo-Ming
authors.initials: RM
authors.lastname: Fang
authors.affiliation: The First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, 510405, China.
authors.forename: Xue-Hong
authors.initials: XH
authors.lastname: Ke
authors.affiliation: The First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, 510405, China.
authors.forename: Yu-Ping
authors.initials: YP
authors.lastname: He
authors.affiliation: The First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, 510405, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Li
authors.affiliation: The First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, 510405, China. fangyq2@163.com.
authors.forename: Yong-Qi
authors.initials: YQ
authors.lastname: Fang
mesh_terms: D000081248:Allylbenzene Derivatives; D000818:Animals; D000840:Anisoles / Q000494:pharmacology*; D017209:Apoptosis / Q000502:physiology; D051017:Apoptosis Regulatory Proteins / Q000378:metabolism; D001343:Autophagy / Q000187:drug effects; D000071186:Beclin-1; D003342:Corpus Striatum / Q000187:drug effects* / Q000378:metabolism; D004298:Dopamine / Q000494:pharmacology*; D020935:MAP Kinase Signaling System / Q000187:drug effects*; D008297:Male; D018696:Neuroprotective Agents / Q000494:pharmacology*; D016627:Oxidopamine / Q000378:metabolism; D020734:Parkinsonian Disorders / Q000188:drug therapy / Q000378:metabolism*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D017207:Rats, Sprague-Dawley
chemical_list: D000081248:Allylbenzene Derivatives; D000840:Anisoles; D051017:Apoptosis Regulatory Proteins; D000071186:Beclin-1; C512829:Becn1 protein, rat; D018696:Neuroprotective Agents; D019253:Proto-Oncogene Proteins c-bcl-2; C012195:asarone; D016627:Oxidopamine; D004298:Dopamine
keywords: Autophagy; Beclin-1; JNK; Neuroprotection; Parkinson’s disease; β-asarone