title: Synthesis and characterization of near IR fluorescent albumin nanoparticles for optical detection of colon cancer.
abstract: Near IR (NIR) fluorescent human serum albumin (HSA) nanoparticles hold great promise as contrast agents for tumor diagnosis. HSA nanoparticles are considered to be biocompatible, non-toxic and non-immunogenic. In addition, NIR fluorescence properties of these nanoparticles are important for in vivo tumor diagnostics, with low autofluorescence and relatively deep penetration of NIR irradiation due to low absorption of biomatrices. The present study describes the synthesis of new NIR fluorescent HSA nanoparticles, by entrapment of a NIR fluorescent dye within the HSA nanoparticles, which also significantly increases the photostability of the dye. Tumor-targeting ligands such as peanut agglutinin (PNA) and anti-carcinoembryonic antigen antibodies (anti-CEA) were covalently conjugated to the NIR fluorescent albumin nanoparticles, increasing the potential fluorescent signal in tumors with upregulated corresponding receptors. Specific colon tumor detection by the NIR fluorescent HSA nanoparticles was demonstrated in a chicken embryo model and a rat model. In future work we also plan to encapsulate cancer drugs such as doxorubicin within the NIR fluorescent HSA nanoparticles for both colon cancer imaging and therapy.
authors.affiliation: Department of Chemistry, Bar-Ilan Institute of Nanotechnology and Advanced Materials, Bar-Ilan University, Ramat-Gan 52900, Israel.
authors.forename: Sarit
authors.initials: S
authors.lastname: Cohen
authors.affiliation: Department of Chemistry, Bar-Ilan Institute of Nanotechnology and Advanced Materials, Bar-Ilan University, Ramat-Gan 52900, Israel.
authors.forename: Michal
authors.initials: M
authors.lastname: Pellach
authors.affiliation: Institute for Drug Research, School of Pharmacy, Faculty of Medicine, The Hebrew University of Jerusalem, P.O. Box 12065, Jerusalem 91120, Israel.
authors.forename: Yossi
authors.initials: Y
authors.lastname: Kam
authors.affiliation: Department of Chemistry, Bar-Ilan Institute of Nanotechnology and Advanced Materials, Bar-Ilan University, Ramat-Gan 52900, Israel.
authors.forename: Igor
authors.initials: I
authors.lastname: Grinberg
authors.affiliation: Department of Chemistry, Bar-Ilan Institute of Nanotechnology and Advanced Materials, Bar-Ilan University, Ramat-Gan 52900, Israel.
authors.forename: Enav
authors.initials: E
authors.lastname: Corem-Salkmon
authors.affiliation: Institute for Drug Research, School of Pharmacy, Faculty of Medicine, The Hebrew University of Jerusalem, P.O. Box 12065, Jerusalem 91120, Israel.
authors.forename: Abraham
authors.initials: A
authors.lastname: Rubinstein
authors.affiliation: Department of Chemistry, Bar-Ilan Institute of Nanotechnology and Advanced Materials, Bar-Ilan University, Ramat-Gan 52900, Israel. Electronic address: shlomo.margel@mail.biu.ac.il.
authors.forename: Shlomo
authors.initials: S
authors.lastname: Margel
mesh_terms: D000418:Albumins / Q000737:chemistry*; D000818:Animals; D002642:Chick Embryo; D002645:Chickens; D003110:Colonic Neoplasms / Q000737:chemistry* / Q000378:metabolism; D005456:Fluorescent Dyes / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D008297:Male; D053758:Nanoparticles / Q000737:chemistry* / Q000378:metabolism; D061848:Optical Imaging / Q000379:methods*; D010316:Particle Size; D051381:Rats; D019265:Spectroscopy, Near-Infrared / Q000379:methods*
chemical_list: D000418:Albumins; D005456:Fluorescent Dyes