title: Vacuolar staining methods in plant cells.
abstract: Commercially available fluorescent dyes enable the fast and specific visualization of plant vacuoles, allowing for investigation of membrane dynamics and vacuolar biogenesis in living cells. Here, we describe different approaches tinting the tonoplast or the vacuolar lumen with a range of dyes, and illustrate its utilization with established fluorescent-tagged marker lines.
authors.affiliation: Department of Applied Genetics and Cell Biology, University of Natural Resources and Life Sciences, Vienna, Muthgasse 18, 1190, Vienna, Austria.
authors.forename: David
authors.initials: D
authors.lastname: Scheuring
authors.forename: Maria
authors.initials: M
authors.lastname: Schöller
authors.forename: Jürgen
authors.initials: J
authors.lastname: Kleine-Vehn
authors.forename: Christian
authors.initials: C
authors.lastname: Löfke
mesh_terms: D017360:Arabidopsis / Q000737:chemistry / Q000166:cytology*; D058948:Cell Tracking / Q000379:methods; D005452:Fluoresceins / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry*; D059828:Plant Cells / Q000737:chemistry*; D011726:Pyridinium Compounds / Q000737:chemistry; D000644:Quaternary Ammonium Compounds / Q000737:chemistry; D013194:Staining and Labeling / Q000379:methods*; D014617:Vacuoles / Q000737:chemistry*
chemical_list: C092350:FM 4-64; D005452:Fluoresceins; D005456:Fluorescent Dyes; D011726:Pyridinium Compounds; D000644:Quaternary Ammonium Compounds; C043829:2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein