title: Oral insulin delivery by self-assembled chitosan nanoparticles: in vitro and in vivo studies in diabetic animal model.
abstract: We have developed self-assembled chitosan/insulin nanoparticles for successful oral insulin delivery. The main purpose of our study is to prepare chitosan/insulin nanoparticles by self-assembly method, to characterize them and to evaluate their efficiency in vivo diabetic model. The size and morphology of the nanoparticles were analyzed by dynamic light scattering (DLS), atomic force microscopy (AFM) and scanning electron microscopy (SEM). The average particle size ranged from 200 to 550 nm, with almost spherical or sub spherical shape. An average insulin encapsulation within the nanoparticles was ~85%. In vitro release study showed that the nanoparticles were also efficient in retaining good amount of insulin in simulated gastric condition, while significant amount of insulin release was noticed in simulated intestinal condition. The oral administrations of chitosan/insulin nanoparticles were effective in lowering the blood glucose level of alloxan-induced diabetic mice. Thus, self-assembled chitosan/insulin nanoparticles show promising effects as potential insulin carrier system in animal models.
authors.affiliation: Department of Polymer Science and Technology, 92, A.P.C. Road, Kolkata-700009, University of Calcutta, India.
authors.forename: Piyasi
authors.initials: P
authors.lastname: Mukhopadhyay
authors.affiliation: Department of Polymer Science and Technology, 92, A.P.C. Road, Kolkata-700009, University of Calcutta, India.
authors.forename: Kishor
authors.initials: K
authors.lastname: Sarkar
authors.affiliation: Department of Physiology, 92, A.P.C. Road, Kolkata-700009, University of Calcutta, India.
authors.forename: Mousumi
authors.initials: M
authors.lastname: Chakraborty
authors.affiliation: Department of Physiology, 92, A.P.C. Road, Kolkata-700009, University of Calcutta, India.
authors.forename: Sourav
authors.initials: S
authors.lastname: Bhattacharya
authors.affiliation: Department of Physiology, 92, A.P.C. Road, Kolkata-700009, University of Calcutta, India.
authors.forename: Roshnara
authors.initials: R
authors.lastname: Mishra
authors.affiliation: Department of Polymer Science and Technology, 92, A.P.C. Road, Kolkata-700009, University of Calcutta, India. Electronic address: ppk923@yahoo.com.
authors.forename: P P
authors.initials: PP
authors.lastname: Kundu
mesh_terms: D000284:Administration, Oral; D000496:Alloxan / Q000633:toxicity; D000818:Animals; D001786:Blood Glucose / Q000032:analysis; D048271:Chitosan / Q000737:chemistry*; D003921:Diabetes Mellitus, Experimental / Q000188:drug therapy; D004195:Disease Models, Animal; D004337:Drug Carriers / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D007328:Insulin / Q000008:administration & dosage / Q000737:chemistry*; D051379:Mice; D018625:Microscopy, Atomic Force; D008855:Microscopy, Electron, Scanning; D053758:Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D001786:Blood Glucose; D004337:Drug Carriers; D007328:Insulin; D000496:Alloxan; D048271:Chitosan