title: Cell death induced by ozone and various non-thermal plasmas: therapeutic perspectives and limitations.
abstract: Non-thermal plasma has been recognized as a promising tool across a vast variety of biomedical applications, with the potential to create novel therapeutic methods. However, the understanding of the molecular mechanisms behind non-thermal plasma cellular effects remains a significant challenge. In this study, we show how two types of different non-thermal plasmas induce cell death in mammalian cell cultures via the formation of multiple intracellular reactive oxygen/nitrogen species. Our results showed a discrepancy in the superoxide accumulation and lysosomal activity in response to air and helium plasma, suggesting that triggered signalling cascades might be grossly different between different plasmas. In addition, the effects of ozone, a considerable component of non-thermal plasma, have been simultaneously evaluated and have revealed much faster and higher cytotoxic effects. Our findings offer novel insight into plasma-induced cellular responses, and provide a basis for better controlled biomedical applications.
authors.affiliation: Institute of Physics AS CR, Prague, Czech Republic.
authors.forename: Oleg
authors.initials: O
authors.lastname: Lunov
authors.affiliation: Institute of Physics AS CR, Prague, Czech Republic.
authors.forename: Vitalii
authors.initials: V
authors.lastname: Zablotskii
authors.affiliation: Institute of Physics AS CR, Prague, Czech Republic.
authors.forename: Olexander
authors.initials: O
authors.lastname: Churpita
authors.affiliation: Institute of Macromolecular Chemistry AS CR, Prague, Czech Republic.
authors.forename: Eliška
authors.initials: E
authors.lastname: Chánová
authors.affiliation: Institute of Experimental Medicine AS CR, Prague, Czech Republic.
authors.forename: Eva
authors.initials: E
authors.lastname: Syková
authors.affiliation: Institute of Physics AS CR, Prague, Czech Republic.
authors.forename: Alexandr
authors.initials: A
authors.lastname: Dejneka
authors.affiliation: 1] Institute of Physics AS CR, Prague, Czech Republic [2] Institute of Experimental Medicine AS CR, Prague, Czech Republic.
authors.forename: Sárka
authors.initials: S
authors.lastname: Kubinová
mesh_terms: D016475:3T3 Cells; D000111:Acetylcysteine / Q000494:pharmacology; D000388:Air; D000818:Animals; D017304:Annexin A5; D000975:Antioxidants / Q000494:pharmacology; D016923:Cell Death / Q000187:drug effects; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D006371:Helium / Q000737:chemistry*; D008247:Lysosomes / Q000187:drug effects; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D051379:Mice; D009457:Neuroglia / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D010126:Ozone / Q000737:chemistry*; D058626:Plasma Gases / Q000494:pharmacology*; D051381:Rats; D026361:Reactive Nitrogen Species / Q000819:agonists / Q000037:antagonists & inhibitors / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000819:agonists / Q000037:antagonists & inhibitors / Q000378:metabolism; D015398:Signal Transduction
chemical_list: D017304:Annexin A5; D000975:Antioxidants; D058626:Plasma Gases; D026361:Reactive Nitrogen Species; D017382:Reactive Oxygen Species; D006371:Helium; D010126:Ozone; D000111:Acetylcysteine