title: Simultaneous heterotrophic nitrification and aerobic denitrification by the marine origin bacterium Pseudomonas sp. ADN-42.
abstract: Recent research has highlighted the existence of some bacteria that are capable of performing heterotrophic nitrification and have a phenomenal ability to denitrify their nitrification products under aerobic conditions. A high-salinity-tolerant strain ADN-42 was isolated from Hymeniacidon perleve and found to display high heterotrophic ammonium removal capability. This strain was identified as Pseudomonas sp. via 16S rRNA gene sequence analysis. Gene cloning and sequencing analysis indicated that the bacterial genome contains N2O reductase function (nosZ) gene. NH3-N removal rate of ADN-42 was very high. And the highest removal rate was 6.52 mg/L · h in the presence of 40 g/L NaCl. Under the condition of pure oxygen (DO >8 mg/L), NH3-N removal efficiency was 56.9 %. Moreover, 38.4 % of oxygen remained in the upper gas space during 72 h without greenhouse gas N2O production. Keeping continuous and low level of dissolved oxygen (DO <3 mg/L) was helpful for better denitrification performance. All these results indicated that the strain has heterotrophic nitrification and aerobic denitrification abilities, which guarantee future application in wastewater treatment.
authors.affiliation: Key Laboratory of Industrial Ecology and Environmental Engineering (Ministry of Education), School of Environmental Science and Technology, Dalian University of Technology, Linggong Road 2, Dalian, 116024, China, jruofei@dlut.edu.cn.
authors.forename: Ruofei
authors.initials: R
authors.lastname: Jin
authors.forename: Tianqi
authors.initials: T
authors.lastname: Liu
authors.forename: Guangfei
authors.initials: G
authors.lastname: Liu
authors.forename: Jiti
authors.initials: J
authors.lastname: Zhou
authors.forename: Jianyu
authors.initials: J
authors.lastname: Huang
authors.forename: Aijie
authors.initials: A
authors.lastname: Wang
mesh_terms: D000332:Aerobiosis; D000641:Ammonia / Q000378:metabolism*; D000818:Animals; D059001:Aquatic Organisms; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D001673:Biodegradation, Environmental; D058440:Denitrification / Q000187:drug effects / Q000502:physiology; D015870:Gene Expression; D020459:Genes, rRNA; D052836:Heterotrophic Processes / Q000502:physiology; D009609:Nitrous Oxide / Q000378:metabolism*; D010088:Oxidoreductases / Q000235:genetics / Q000378:metabolism*; D010100:Oxygen / Q000494:pharmacology; D010802:Phylogeny; D011161:Porifera / Q000382:microbiology; D011549:Pseudomonas / Q000145:classification / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D055049:Salt Tolerance / Q000502:physiology; D012965:Sodium Chloride / Q000494:pharmacology; D062065:Wastewater / Q000382:microbiology
chemical_list: D000641:Ammonia; D001426:Bacterial Proteins; D009609:Nitrous Oxide; D010088:Oxidoreductases; D010100:Oxygen; D012336:RNA, Ribosomal, 16S; D012965:Sodium Chloride; D062065:Wastewater; C022763:nitrous oxide reductase