title: Experimentally induced psoriatic lesions associate with rapid but transient decrease in interleukin-33 immunostaining in epidermis.
abstract: A slight epidermal damage can induce the Köbner reaction in psoriasis, and the “alarmin”, interleukin-33 (IL-33), may be involved in this process. Therefore, the uninvolved psoriatic skin was tape-stripped, and skin biopsies were collected at 0 day, 2 h and 3 days or at 0 day, 1 day and 7 days for immunohistochemistry. Eight patients out of 18 with the positive Köbner reaction showed a decrease in epidermal thickness and revealed transient reduction in epidermal nuclear immunostaining of IL-33 in 2-h, 1-day, 3-day biopsies compared to the 10 Köbner-negative patients. In keratinocyte cultures, the full-length 32-kDa IL-33 was detected after damaging the cells with freeze-thawing. Interestingly, a very low concentration of rh-IL-33 (0.001–0.01 ng/ml) significantly stimulated (3)H-thymidine uptake by human LAD2 mast cells, but not by psoriatic peripheral blood mononuclear cells. The results show that epidermal IL-33 associates with positive Köbner response, and only a small amount of the IL-33 apparently released may induce proliferation in dermal mast cells.
authors.affiliation: Department of Dermatology, University of Eastern Finland and Kuopio University Hospital, 70029 Kuopio, Finland.
authors.forename: Mireille-Maria
authors.initials: MM
authors.lastname: Suttle
authors.forename: Mattias
authors.initials: M
authors.lastname: Enoksson
authors.forename: Anna
authors.initials: A
authors.lastname: Zoltowska
authors.forename: Madhumita
authors.initials: M
authors.lastname: Chatterjee
authors.forename: Gunnar
authors.initials: G
authors.lastname: Nilsson
authors.forename: Ilkka T
authors.initials: IT
authors.lastname: Harvima
mesh_terms: D000328:Adult; D000368:Aged; D001707:Biopsy, Needle; D015153:Blotting, Western; D049109:Cell Proliferation; D015331:Cohort Studies; D004797:Enzyme-Linked Immunosorbent Assay; D004817:Epidermis / Q000276:immunology* / Q000473:pathology*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D000067596:Interleukin-33 / Q000276:immunology / Q000378:metabolism*; D015603:Keratinocytes / Q000166:cytology / Q000276:immunology; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000502:physiology; D008297:Male; D008407:Mast Cells / Q000276:immunology / Q000502:physiology; D008875:Middle Aged; D011565:Psoriasis / Q000276:immunology* / Q000473:pathology*; D012016:Reference Values; D012680:Sensitivity and Specificity; D012720:Severity of Illness Index; D012882:Skin Tests / Q000379:methods; D013194:Staining and Labeling; D055815:Young Adult
chemical_list: D000067596:Interleukin-33