title: Osteoactivin attenuates skeletal muscle fibrosis after distraction osteogenesis by promoting extracellular matrix degradation/remodeling.
abstract: The aim of this study was to determine whether osteoactivin attenuated skeletal muscle fibrosis caused by distraction osteogenesis. Tibial osteotomies were performed on wild-type and osteoactivin-transgenic (OA-Tg) mice, and tibiae were distracted for 2 weeks. Ankle plantar flexion torque and the gastrocnemius muscles were analyzed. The amount and area of collagenous tissue and the passive torque were reduced in the OA-Tg group at 8 weeks after osteotomy. Transcript levels of matrix metalloprotease (mmp)-3 and MMP-9 were upregulated, and MMP-3 and MMP-9 proteins were increased in the OA-Tg group. Osteoactivin-mediated increase in MMPs may attenuate skeletal muscle fibrosis.
authors.affiliation: aDepartment of Orthopedics bDepartment of Nutritional Physiology, Institute of Health Biosciences, The University of Tokushima Graduate School, Tokushima, Japan.
authors.forename: Ichiro
authors.initials: I
authors.lastname: Tonogai
authors.forename: Mitsuhiko
authors.initials: M
authors.lastname: Takahashi
authors.forename: Kiminori
authors.initials: K
authors.lastname: Yukata
authors.forename: Ryosuke
authors.initials: R
authors.lastname: Sato
authors.forename: Takeshi
authors.initials: T
authors.lastname: Nikawa
authors.forename: Natsuo
authors.initials: N
authors.lastname: Yasui
authors.forename: Koichi
authors.initials: K
authors.lastname: Sairyo
mesh_terms: D000818:Animals; D000843:Ankle Joint / Q000502:physiology; D005109:Extracellular Matrix / Q000502:physiology*; D005136:Eye Proteins / Q000502:physiology*; D005355:Fibrosis / Q000209:etiology / Q000503:physiopathology*; D008297:Male; D019278:Matrix Metalloproteinase 3 / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D008562:Membrane Glycoproteins / Q000502:physiology*; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D053580:Muscle Strength / Q000502:physiology*; D018482:Muscle, Skeletal / Q000502:physiology*; D019857:Osteogenesis, Distraction / Q000009:adverse effects*; D010027:Osteotomy; D013977:Tibia / Q000601:surgery
chemical_list: D005136:Eye Proteins; C445671:Gpnmb protein, mouse; D008562:Membrane Glycoproteins; D019278:Matrix Metalloproteinase 3; D020780:Matrix Metalloproteinase 9