title: [Cloning, expression and immuno-protection analysis of a gene encoding troponin T of Schistosoma japonicum (SjTnT)].
abstract: OBJECTIVE
  To clone cDNA encoding troponin T of Schistosoma japonicum (SjTnT), and evaluate the protective efficacy induced by recombinant SjTnT in BALB/c mice against S. japonicum challenge infection.
  
  
  METHODS
  The SjTnT gene was amplified from 28-day-schistosome cDNAs by PCR and then subcloned into pET28a(+). The recombinant SjTnT protein (rSjTnT) was expressed in Escherichia coli BL21 (DE3) cells. The serum specific to rSjTnT was prepared by immunized BALB/c mice with the recombinant antigen, and the immunogenicity of rSjTnT was detected by Western blotting and ELISA. The immuno-protective efficacy induced by rSjTnT in BALB/c mice was evaluated according to the reduction in worm and egg counts.
  
  
  RESULTS
  The cDNA encoding SjTnT was successfully cloned and expressed in E. coli. Western blotting showed that rSjTnT had a good immunogenicity. The high level of specific IgG antibodies was detected, and 33.89% worm reduction and 43.94% liver egg reduction were obtained in mice vaccinated with rSjTnT combined with Seppic 206 adjuvant compared with those in the adjuvant control group.
  
  
  CONCLUSIONS
  rSjTnT could induce partial immuno-protection against S. japonicum infection in BALB/c mice. This study provided a basic for understanding the biological function of SjTnT.
authors.forename: Xin-zhuo
authors.initials: XZ
authors.lastname: Wang
authors.forename: Yang
authors.initials: Y
authors.lastname: Hong
authors.forename: Hong-xiao
authors.initials: HX
authors.lastname: Han
authors.forename: Chang-jian
authors.initials: CJ
authors.lastname: Li
authors.forename: Xiao-dan
authors.initials: XD
authors.lastname: Cao
authors.forename: Ke
authors.initials: K
authors.lastname: Lu
authors.forename: Hao
authors.initials: H
authors.lastname: Li
authors.lastname: Zhu Chuan-gang
authors.forename: Chuan-gang
authors.initials: CG
authors.lastname: Fu
authors.forename: Jiao-jiao
authors.initials: JJ
authors.lastname: Lin
mesh_terms: D000818:Animals; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D005260:Female; D015870:Gene Expression; D007073:Immunoglobulin E / Q000276:immunology; D008297:Male; D051379:Mice; D010957:Plasmids / Q000235:genetics; D011817:Rabbits; D012549:Schistosoma japonicum / Q000235:genetics*; D020107:Troponin T / Q000235:genetics* / Q000276:immunology* / Q000302:isolation & purification
chemical_list: D020107:Troponin T; D007073:Immunoglobulin E