title: Epithelial rotation promotes the global alignment of contractile actin bundles during Drosophila egg chamber elongation.
abstract: Tissues use numerous mechanisms to change shape during development. The Drosophila egg chamber is an organ-like structure that elongates to form an elliptical egg. During elongation the follicular epithelial cells undergo a collective migration that causes the egg chamber to rotate within its surrounding basement membrane. Rotation coincides with the formation of a 'molecular corset', in which actin bundles in the epithelium and fibrils in the basement membrane are all aligned perpendicular to the elongation axis. Here we show that rotation plays a critical role in building the actin-based component of the corset. Rotation begins shortly after egg chamber formation and requires lamellipodial protrusions at each follicle cell's leading edge. During early stages, rotation is necessary for tissue-level actin bundle alignment, but it becomes dispensable after the basement membrane is polarized. This work highlights how collective cell migration can be used to build a polarized tissue organization for organ morphogenesis.
authors.affiliation: 1] Department of Molecular Genetics and Cell Biology, The University of Chicago, 920 East 58th Street, Chicago, Illinois 60637, USA [2] Committee on Development, Regeneration and Stem Cell Biology, The University of Chicago, 920 East 58th Street, Chicago, Illinois 60637, USA.
authors.forename: Maureen
authors.initials: M
authors.lastname: Cetera
authors.affiliation: 1] Department of Molecular Genetics and Cell Biology, The University of Chicago, 920 East 58th Street, Chicago, Illinois 60637, USA [2] Institute for Biophysical Dynamics, James Franck Institute and Department of Physics, The University of Chicago, 929 East 57th Street, Chicago, Illinois 60637, USA.
authors.forename: Guillermina R
authors.initials: GR
authors.lastname: Ramirez-San Juan
authors.affiliation: Institute for Biophysical Dynamics, James Franck Institute and Department of Physics, The University of Chicago, 929 East 57th Street, Chicago, Illinois 60637, USA.
authors.forename: Patrick W
authors.initials: PW
authors.lastname: Oakes
authors.affiliation: 1] Department of Molecular Genetics and Cell Biology, The University of Chicago, 920 East 58th Street, Chicago, Illinois 60637, USA [2] Department of Biological Sciences, Butler University, 4600 Sunset Boulevard, Indianapolis, Indiana 46208, USA.
authors.forename: Lindsay
authors.initials: L
authors.lastname: Lewellyn
authors.affiliation: Life Sciences Centre, Department of Cellular and Physiological Sciences, University of British Columbia, 2350 Health Sciences Mall, Vancouver, British Columbia, Canada V6T 1Z3.
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Fairchild
authors.affiliation: Life Sciences Centre, Department of Cellular and Physiological Sciences, University of British Columbia, 2350 Health Sciences Mall, Vancouver, British Columbia, Canada V6T 1Z3.
authors.forename: Guy
authors.initials: G
authors.lastname: Tanentzapf
authors.affiliation: Institute for Biophysical Dynamics, James Franck Institute and Department of Physics, The University of Chicago, 929 East 57th Street, Chicago, Illinois 60637, USA.
authors.forename: Margaret L
authors.initials: ML
authors.lastname: Gardel
authors.affiliation: 1] Department of Molecular Genetics and Cell Biology, The University of Chicago, 920 East 58th Street, Chicago, Illinois 60637, USA [2] Committee on Development, Regeneration and Stem Cell Biology, The University of Chicago, 920 East 58th Street, Chicago, Illinois 60637, USA.
authors.forename: Sally
authors.initials: S
authors.lastname: Horne-Badovinac
mesh_terms: D000199:Actins; D000818:Animals; D015820:Cadherins / Q000235:genetics; D002352:Carrier Proteins / Q000235:genetics; D002465:Cell Movement; D016764:Cell Polarity; D003285:Contractile Proteins / Q000378:metabolism*; D029721:Drosophila Proteins / Q000235:genetics; D004331:Drosophila melanogaster / Q000196:embryology*; D005136:Eye Proteins / Q000235:genetics; D018398:Homeodomain Proteins / Q000235:genetics; D008840:Microfilament Proteins / Q000235:genetics; D009024:Morphogenesis; D009866:Oogenesis / Q000235:genetics*; D010063:Ovum / Q000254:growth & development*; D011554:Pseudopodia / Q000378:metabolism*; D034622:RNA Interference; D034741:RNA, Small Interfering; D014157:Transcription Factors / Q000235:genetics
chemical_list: C121808:Abi protein, Drosophila; D000199:Actins; D015820:Cadherins; D002352:Carrier Proteins; D003285:Contractile Proteins; D029721:Drosophila Proteins; D005136:Eye Proteins; C557617:GAL4 protein, Drosophila; D018398:Homeodomain Proteins; D008840:Microfilament Proteins; D034741:RNA, Small Interfering; C449671:SCAR protein, Drosophila; D014157:Transcription Factors; C546978:kug protein, Drosophila; C105790:mirr protein, Drosophila