title: Metabolomics-based approach for assessing the toxicity mechanisms of dibutyl phthalate to abalone (Haliotis diversicolor supertexta).
abstract: Dibutyl phthalate (DBP) is a ubiquitous contaminant in the marine environment, and relatively little is known about the toxicological mechanisms of this compound at the metabolite level. In this study, marine gastropods (abalone) were exposed to DBP at environmentally relevant concentrations (2, 10, and 50 μg/L) for 30 days. The plasma metabolite profiles were determined at the 5th, 15th, and 30th. The major metabolite changes corresponding to DBP exposure were related to osmotic regulation, energy metabolism, and environmental stress, and the effects displayed a dose-dependent pattern. The most obvious change was the increase in the levels of intracellular metabolites (betaine, dimethylglycine, homarine, glutamine, and lactate) and tricarboxylic acid cycle intermediates. The results revealed that DBP may lead to abalone oxidative stress, lipid metabolism dysfunction, energy metabolism disturbance, and osmoregulation imbalance. These results would be helpful in better understanding the mechanisms of abalone response to DBP stress at the system level.
authors.affiliation: The Division of Ocean Science and Technology, Graduate School at Shenzhen, Tsinghua University, Room 304, Building L, Shenzhen University Town, Xili, Shenzhen City, 518055, People's Republic of China.
authors.forename: Jin
authors.initials: J
authors.lastname: Zhou
authors.forename: Baiyang
authors.initials: B
authors.lastname: Chen
authors.forename: Zhonghua
authors.initials: Z
authors.lastname: Cai
mesh_terms: D000818:Animals; D003993:Dibutyl Phthalate / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D052244:Endocrine Disruptors / Q000633:toxicity*; D004734:Energy Metabolism / Q000187:drug effects; D005260:Female; D049851:Gastropoda / Q000187:drug effects* / Q000378:metabolism; D050356:Lipid Metabolism / Q000187:drug effects; D009682:Magnetic Resonance Spectroscopy; D055432:Metabolomics / Q000379:methods*; D018384:Oxidative Stress / Q000187:drug effects*; D018675:Toxicity Tests; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D052244:Endocrine Disruptors; D014874:Water Pollutants, Chemical; D003993:Dibutyl Phthalate