title: Effect of autologous adipose tissue-derived mesenchymal stem cells on neovascularization of artificial equine tendon lesions.
abstract: OBJECTIVE
  To investigate whether autologous adipose tissue-derived mesenchymal stem cells (AT-MSCs) treatment of tendon lesions increases neovascularization during tendon healing.
  
  
  METHODS
  A standardized surgical model was used to create lesions in both front limb superficial digital flexor tendons (SDFTs) of nine horses. Either AT-MSCs or control substance was injected intralesionally 2 weeks post-surgery. Color Doppler ultrasonography of SDFTs was performed at regular intervals. Horses were euthanized 22 weeks post-treatment and SDFTs were harvested for histology.
  
  
  RESULTS
  The color Doppler ultrasonography signal was significantly more extensive at 2 weeks post-treatment and the number of vessels counted on histologic slides was significantly higher at 22 weeks post-treatment in AT-MSC-treated SDFTs.
  
  
  CONCLUSIONS
  Our findings indicate that AT-MSC treatment has a beneficial effect on neovascularization of healing tendons.
authors.affiliation: Clinic for Horses, University of Veterinary Medicine Hannover, Foundation, Hannover, Bünteweg 9, 30559 Hannover, Germany.
authors.forename: Philipp
authors.initials: P
authors.lastname: Conze
authors.forename: Hans T M
authors.initials: HT
authors.lastname: van Schie
authors.forename: René
authors.initials: R
authors.lastname: van Weeren
authors.forename: Carsten
authors.initials: C
authors.lastname: Staszyk
authors.forename: Sabine
authors.initials: S
authors.lastname: Conrad
authors.forename: Thomas
authors.initials: T
authors.lastname: Skutella
authors.forename: Klaus
authors.initials: K
authors.lastname: Hopster
authors.forename: Karl
authors.initials: K
authors.lastname: Rohn
authors.forename: Peter
authors.initials: P
authors.lastname: Stadler
authors.forename: Florian
authors.initials: F
authors.lastname: Geburek
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D002478:Cells, Cultured; D006734:Horse Diseases / Q000473:pathology / Q000628:therapy*; D006736:Horses; D007124:Immunoenzyme Techniques; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D018919:Neovascularization, Physiologic; D013708:Tendon Injuries / Q000473:pathology / Q000628:therapy*; D013710:Tendons / Q000098:blood supply*; D014182:Transplantation, Autologous
keywords: Factor VIII; adipose tissue-derived; color Doppler ultrasonography; mesenchymal stem cells; neovascularization; superficial digital flexor tendon; von Willebrand factor