title: Techniques to access histone modifications and variants in cancer.
abstract: Recent years have witnessed an explosion of epigenetic research on the role of histone variants and modifications in cancer. To understand the global dynamics of chromatin structure and function, analysis of histone variants incorporated into the nucleosome and their covalent modifications, is required. The nucleosome is the fundamental structural unit of chromatin, contains an octamer of core histones H3, H4, H2A, and H2B. The differential alterations in diverse histone variants and their accompanying modifications patterns will provide a deeper insight into their biological role in structural and functional properties of chromatin. Here we provide a step-by-step protocol to investigate these aspects, the histone modifications and variants, their localization and dynamics within specific regions of chromatin under distinct condition and the recruitment/retention of epigenetic regulators at their target sites in chromatin to influence cell growth and differentiation.
authors.affiliation: Epigenetics and Chromatin Biology Group, Gupta Laboratory, Cancer Research Institute, Advanced Centre for Treatment, Research and Education in Cancer, Tata Memorial Centre, Kharghar, Navi Mumbai, 410210, MH, India.
authors.forename: Monica
authors.initials: M
authors.lastname: Tyagi
authors.forename: Shafqat A
authors.initials: SA
authors.lastname: Khan
authors.forename: Saikat
authors.initials: S
authors.lastname: Bhattacharya
authors.forename: Divya
authors.initials: D
authors.lastname: Reddy
authors.forename: Ajit K
authors.initials: AK
authors.lastname: Sharma
authors.forename: Bharat
authors.initials: B
authors.lastname: Khade
authors.forename: Sanjay
authors.initials: S
authors.lastname: Gupta
mesh_terms: D045744:Cell Line, Tumor; D002467:Cell Nucleus / Q000235:genetics; D047369:Chromatin Immunoprecipitation; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D036681:Fluorescence Recovery After Photobleaching; D005455:Fluorescent Antibody Technique; D005898:Glass / Q000737:chemistry; D006657:Histones / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008836:Micrococcal Nuclease / Q000378:metabolism; D009369:Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D009195:Peroxidase / Q000378:metabolism; D055503:Protein Multimerization; D011499:Protein Processing, Post-Translational; D020836:Protein Structure, Quaternary; D013194:Staining and Labeling
chemical_list: D006657:Histones; D009195:Peroxidase; D008836:Micrococcal Nuclease