title: Precise quantitative addition of multiple reagents into droplets in sequence using glass fiber-induced droplet coalescence.
abstract: Precise quantitative addition of multiple reagents into droplets in sequence is still a bottleneck in droplet-based analysis. To address this issue, we presented a simple and robust glass fiber-induced droplet coalescence method. The hydrophilic glass fiber embedded in the microchannels can induce the deformation of droplets and trigger the coalescence. Serial addition of reagents with controlled volumes was performed by this method without the requirement for an external power source.
authors.affiliation: Single-Cell Center, CAS Key Laboratory of Biofuels and Shandong Key Laboratory of Energy Genetics, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, Shandong 266101, China. mabo@qibebt.ac.cn xujian@qibebt.ac.cn.
authors.forename: Chunyu
authors.initials: C
authors.lastname: Li
authors.forename: Jian
authors.initials: J
authors.lastname: Xu
authors.forename: Bo
authors.initials: B
authors.lastname: Ma
mesh_terms: D002712:Chlorides / Q000737:chemistry; D004655:Emulsions; D005290:Ferric Compounds / Q000737:chemistry; D005898:Glass / Q000737:chemistry*; D057927:Hydrophobic and Hydrophilic Interactions; D007202:Indicators and Reagents; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods*; D010316:Particle Size; D013499:Surface Properties; D013501:Surface-Active Agents / Q000737:chemistry*; D013861:Thiocyanates / Q000737:chemistry; D014867:Water / Q000737:chemistry
chemical_list: D002712:Chlorides; D004655:Emulsions; D005290:Ferric Compounds; D007202:Indicators and Reagents; D013501:Surface-Active Agents; D013861:Thiocyanates; C009753:fiberglass; D014867:Water; C009941:potassium thiocyanate; C024555:ferric chloride