title: Refeeding with conjugated linoleic acid increases serum cholesterol and modifies the fatty acid profile after 48 hours of fasting in rats.
abstract: There is no consensus about the effects of conjugated linoleic acid (CLA) on lipid metabolism, especially in animals fed a high-fat diet. Therefore, the objective of the present study was to evaluate the incorporation of CLA isomers into serum, liver and adipose tissue, as well as the oxidative stress generated in rats refed with high-fat diets after a 48 hour fast. Rats were refed with diets containing soybean oil, rich in linoleic acid [7% (Control Group - C) or 20% (LA Group)], CLA [CLA Group - 20% CLA mixture (39.32 mole% c9,t11-CLA and 40.59 mole% t10,c12- CLA)], soybean oil + CLA (LA+CLA Group - 15.4% soybean oil and 4.6% CLA) or animal fat (AF, 20% lard). The CLA group showed lower weight gain and liver weight after refeeding, as well as increased serum cholesterol. The high dietary fat intake induced fat accumulation and an increase in -tocopherol in the liver, which were not observed in the CLA group. Circulating -tocopherol was increased in the CLA and CLA+LA groups. The high- fat diets reduced liver catalase activity. CLA isomers were incorporated into serum and tissues. In this shortterm refeeding experimental model, CLA prevented hepatic fat accumulation, although it produced an increase in serum cholesterol.
authors.affiliation: Department of Internal Medicine, Faculty of Medicine of Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP. Brazil.. gabriela.castro@usp.br.
authors.forename: Gabriela Salim
authors.initials: GS
authors.lastname: de Castro
authors.affiliation: Food Science and Nutrition, Department of Biological Sciences, School of Biochemistry and Biological Sciences, Universidad Nacional del Litoral, Santa Fe. Argentina.. mfandreoli@hotmail.com.
authors.forename: María Florencia
authors.initials: MF
authors.lastname: Andreoli
authors.affiliation: Food Science and Nutrition, Department of Biological Sciences, School of Biochemistry and Biological Sciences, Universidad Nacional del Litoral, Santa Fe. Argentina.. paolaillesca@hotmail.com.
authors.forename: Paola G
authors.initials: PG
authors.lastname: Illesca
authors.affiliation: Department of Internal Medicine, Faculty of Medicine of Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP. Brazil.. ppayao@hotmail.com.
authors.forename: Paula
authors.initials: P
authors.lastname: Payão Ovídio
authors.affiliation: Food Science and Nutrition, Department of Biological Sciences, School of Biochemistry and Biological Sciences, Universidad Nacional del Litoral, Santa Fe. Argentina.. cbernal@fbcb.unl.edu.ar.
authors.forename: Claudio A
authors.initials: CA
authors.lastname: Bernal
authors.affiliation: Department of Internal Medicine, Faculty of Medicine of Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP. Brazil.. alceu@fmrp.usp.br.
authors.forename: Alceu A
authors.initials: AA
authors.lastname: Jordão
authors.affiliation: Department of Internal Medicine, Faculty of Medicine of Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP. Brazil.. hvannucc@fmrp.usp.br.
authors.forename: Helio
authors.initials: H
authors.lastname: Vannucchi
mesh_terms: D000818:Animals; D002784:Cholesterol / Q000097:blood*; D059305:Diet, High-Fat; D005215:Fasting / Q000378:metabolism; D005227:Fatty Acids / Q000097:blood*; D044243:Linoleic Acids, Conjugated / Q000494:pharmacology*; D050356:Lipid Metabolism / Q000187:drug effects; D008099:Liver / Q000378:metabolism; D008297:Male; D051381:Rats; D017208:Rats, Wistar
chemical_list: D005227:Fatty Acids; D044243:Linoleic Acids, Conjugated; D002784:Cholesterol