title: Expression of interleukine-8 as an independent prognostic factor for sporadic colon cancer dissemination.
abstract: OBJECTIVE
  The aim of our study was to investigate the gene and serum protein expression profiles of IL-8 in colon cancer and associated hepatic metastasis and to correlate these results with clinicopathologic variables of the patients.
  
  
  METHODS
  IL-8 was evaluated by qPCR and ELISA in a total number of 62 colon cancer patients (n=42 by qPCR and n=20 by ELISA) in normal and tumoral tissue specimens and serum samples respectively. Additionally synchronous metastasis from 5 of these patients were also collected at the time of surgery and analyzed by qPCR.
  
  
  RESULTS
  IL-8 was up regulated in all analyzed tumoral samples compared with normal tissue (P-value = 0.01) and higher expressed in metastatic tissues compared with tumoral tissues (P -value= 0.03). The median expression of IL-8 in patients over 60 years old was found to be higher compared with the median expression of IL8 in patients less than 60 years old (3.89 compared with 14.69, P -value= 0.005). According to tumor grading, we found that IL-8 in tumors with well differentiated adenocarcinoma have a median mRNA expression of 9.78 compared with a median mRNA IL8 expression of 26.63 in moderate or poor differentiated adenocarcinoma. Levels of IL-8 determined in serum were statistically significant correlated with preoperative carcinoembryonic antigen level (P -value= 0.003, R=0.57) and with distant metastasis (P-value =0.008). Serum level of IL-8 increased proportionally along with TNM tumor stage and was found to be statistically significant correlated with C-reactive protein (P -value, R=0.64). Colon cancer patients had higher IL-8 levels as determined by ELISA (median value= 29.64 pg/ml) compared with healthy controls (median value= 4.86 pg/ml).
  
  
  CONCLUSIONS
  Our results provide additional support for the role of inflammation in colon cancer and indicate that IL-8 could be further validated in association with other already used markers for prognostic and diagnostic of evolutional disease in colon cancer patients.
authors.affiliation: Fundeni Clinical Institute, Bucharest, Romania The two authors had an equal contribution in the article.
authors.forename: A
authors.initials: A
authors.lastname: Nastase
authors.affiliation: Fundeni Clinical Institute, Bucharest, Romania The two authors had an equal contribution in the article.
authors.forename: L
authors.initials: L
authors.lastname: Paslaru
authors.affiliation: Fundeni Clinical Institute, Bucharest, Romania The two authors had an equal contribution in the article.
authors.forename: V
authors.initials: V
authors.lastname: Herlea
authors.affiliation: Fundeni Clinical Institute, Bucharest, Romania The two authors had an equal contribution in the article.
authors.forename: M
authors.initials: M
authors.lastname: Ionescu
authors.affiliation: Fundeni Clinical Institute, Bucharest, Romania The two authors had an equal contribution in the article.
authors.forename: D
authors.initials: D
authors.lastname: Tomescu
authors.affiliation: Fundeni Clinical Institute, Bucharest, Romania The two authors had an equal contribution in the article.
authors.forename: N
authors.initials: N
authors.lastname: Bacalbasa
authors.affiliation: Fundeni Clinical Institute, Bucharest, Romania The two authors had an equal contribution in the article.
authors.forename: S
authors.initials: S
authors.lastname: Dima
authors.affiliation: Fundeni Clinical Institute, Bucharest, Romania The two authors had an equal contribution in the article.
authors.forename: I
authors.initials: I
authors.lastname: Popescu
mesh_terms: D000367:Age Factors; D000368:Aged; D014408:Biomarkers, Tumor / Q000097:blood / Q000378:metabolism*; D002272:Carcinoembryonic Antigen / Q000097:blood; D003110:Colonic Neoplasms / Q000097:blood / Q000175:diagnosis* / Q000378:metabolism* / Q000473:pathology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000502:physiology*; D006801:Humans; D016209:Interleukin-8 / Q000097:blood / Q000378:metabolism*; D008113:Liver Neoplasms / Q000556:secondary*; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D011379:Prognosis; D018709:Statistics, Nonparametric; D059467:Transcriptome
chemical_list: D014408:Biomarkers, Tumor; D002272:Carcinoembryonic Antigen; D016209:Interleukin-8
keywords: biomarkers; colon cancer; interleukine-8; metastasis; qPCR