title: Combining acellular nerve allografts with brain-derived neurotrophic factor transfected bone marrow mesenchymal stem cells restores sciatic nerve injury better than either intervention alone.
abstract: In this study, we chemically extracted acellular nerve allografts from bilateral sciatic nerves, and repaired 10-mm sciatic nerve defects in rats using these grafts and brain-derived neurotrophic factor transfected bone marrow mesenchymal stem cells. Experiments were performed in three groups: the acellular nerve allograft bridging group, acellular nerve allograft + bone marrow mesenchymal stem cells group, and the acellular nerve allograft + brain-derived neurotrophic factor transfected bone marrow mesenchymal stem cells group. Results showed that at 8 weeks after bridging, sciatic functional index, triceps wet weight recovery rate, myelin thickness, and number of myelinated nerve fibers were significantly changed in the three groups. Variations were the largest in the acellular nerve allograft + brain-derived neurotrophic factor transfected bone marrow mesenchymal stem cells group compared with the other two groups. Experimental findings suggest that chemically extracted acellular nerve allograft combined nerve factor and mesenchymal stem cells can promote the restoration of sciatic nerve defects. The repair effect seen is better than the single application of acellular nerve allograft or acellular nerve allograft combined mesenchymal stem cell transplantation.
authors.affiliation: School of International Education, Zhengzhou University, Zhengzhou, Henan Province, China ; Institute of Clinical Anatomy, Southern Medical University, Guangzhou, Guangdong Province, China.
authors.forename: Yanru
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Orthopedic Surgery, First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan Province, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Department of Orthopedic Surgery, First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan Province, China.
authors.forename: Gechen
authors.initials: G
authors.lastname: Zhang
authors.affiliation: School of International Education, Zhengzhou University, Zhengzhou, Henan Province, China.
authors.forename: Ka
authors.initials: K
authors.lastname: Ka
authors.affiliation: Institute of Clinical Anatomy, Southern Medical University, Guangzhou, Guangdong Province, China.
authors.forename: Wenhua
authors.initials: W
authors.lastname: Huang
keywords: bone marrow mesenchymal stem cells; brain-derived neurotrophic factor; chemically extracted acellular nerve; nerve regeneration; nerve tissue engineering; neural regeneration; peripheral nerve injury; peripheral nerve regeneration