title: Morphological and chemical comparative analysis of the human and bovine dentin-adhesive layer.
abstract: Micro energy-dispersive X-ray fluorescence spectrometry (µ-EDXRF) and scanning electron microscopy (SEM) were used to evaluate dentin treated with an etch and rinse adhesive (ER) and a self-etch adhesive (SE). Ten human molars (H) and ten bovine anterior teeth (B) were prepared (exposure of dentin and divided in the middle) and allocated into two different adhesion strategy groups per substrate (n=40). µ-EDXRF data and SEM images were obtained before and after treatment. Untreated dentin of both substrates did not differ in terms of Ca (p<0.1503), P (p<0.2986) or Ca/P ratio (p<0.1400). H-SE and B-SE specimens showed reduced P content (p<0.0001; p<0.0002), while H-ER and B-ER specimens showed reduced Ca and P content (p<0.0001; p<0.0001) when compared with untreated specimens. The Ca/P ratio was significantly higher in H-ER and B-ER specimens than in H-SE and B-SE specimens (p<0.0001; p<0.0080). Untreated dentin showed a homogeneous elemental distribution. However, after adhesive treatments, the surface of the dentin showed an irregular demineralization pattern. The resin tags and adhesive layer were shorter in bovine specimens than in human specimens due to morphological differences.
authors.affiliation: 1Department of Dental Materials and Operative Dentistry,School of Dentistry,University of Vale do Paraíba,UNIVAP,São José dos Campos,SP 12.244-000,Brazil.
authors.forename: Luís Eduardo Silva
authors.initials: LE
authors.lastname: Soares
authors.affiliation: 3Departamento de Ciências Exatas e da Terra,Universidade Federal de São Paulo,UNIFESP,Diadema,São Paulo 09972-270,Brazil.
authors.forename: Ana Maria do Espírito
authors.initials: AM
authors.lastname: Santo
mesh_terms: D000269:Adhesives / Q000737:chemistry*; D000818:Animals; D002417:Cattle; D003804:Dentin / Q000033:anatomy & histology* / Q000648:ultrastructure; D017220:Dentin-Bonding Agents / Q000737:chemistry; D006801:Humans; D008855:Microscopy, Electron, Scanning; D019279:Resin Cements / Q000737:chemistry; D013052:Spectrometry, X-Ray Emission; D014070:Tooth / Q000033:anatomy & histology / Q000648:ultrastructure
chemical_list: D000269:Adhesives; D017220:Dentin-Bonding Agents; D019279:Resin Cements
keywords: scanning electron microscopy