title: Voltammetric detection of anti-HIV replication drug based on novel nanocomposite gold-nanoparticle-CaCO3 hybrid material.
abstract: A novel bionanocomposite, horse radish peroxidase- gold-nanoparticle-Calcium carbonate (HRP-AuNPs-CaCO3), hybrid material was encapsulated by silica sol on a glassy carbon electrode (GCE). The fabricated modified electrode was used as a novel voltammetric sensor for electrochemical sensing of anti-HIV replication drug i.e. deferiprone. The surface morphology of the modified electrode was characterized by scanning electron microscopy (SEM). Results obtained from the voltammetric measurements show that HRP-AuNPs-CaCO3 modified GCE offers a selective and sensitive electrochemical sensor for the determination of deferiprone. Under experimental conditions, the proposed voltammetric sensor has a linear response range from 0.01 to 10,000 μM with a detection limit of 0.01 μM. Furthermore, the fabricated sensor was successfully applied to determine deferiprone level in spiked urine and serum samples.
authors.affiliation: Amity Institute of Nanotechnology, AMITY University, Noida, UP, India, jags_biotech@yahoo.co.in.
authors.forename: Jagriti
authors.initials: J
authors.lastname: Narang
authors.forename: Nitesh
authors.initials: N
authors.lastname: Malhotra
authors.forename: Gajendra
authors.initials: G
authors.lastname: Singh
authors.forename: C S
authors.initials: CS
authors.lastname: Pundir
mesh_terms: D019380:Anti-HIV Agents / Q000032:analysis / Q000097:blood / Q000652:urine; D015374:Biosensing Techniques; D002119:Calcium Carbonate / Q000737:chemistry*; D000077543:Deferiprone; D016903:Drug Monitoring / Q000379:methods*; D055664:Electrochemical Techniques; D004566:Electrodes; D006046:Gold / Q000737:chemistry*; D006108:Graphite / Q000737:chemistry; D006735:Horseradish Peroxidase / Q000737:chemistry; D006801:Humans; D057230:Limit of Detection; D008422:Materials Testing; D053768:Metal Nanoparticles / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D053761:Nanocomposites / Q000737:chemistry*; D036103:Nanotechnology; D044367:Phase Transition; D011728:Pyridones / Q000032:analysis / Q000097:blood / Q000652:urine; D012822:Silicon Dioxide / Q000737:chemistry; D013696:Temperature
chemical_list: D019380:Anti-HIV Agents; D011728:Pyridones; D000077543:Deferiprone; D006046:Gold; D012822:Silicon Dioxide; D006108:Graphite; D006735:Horseradish Peroxidase; D002119:Calcium Carbonate