title: Enteroaggregative Escherichia coli have evolved independently as distinct complexes within the E. coli population with varying ability to cause disease.
abstract: Enteroaggregative E. coli (EAEC) is an established diarrhoeagenic pathotype. The association with virulence gene content and ability to cause disease has been studied but little is known about the population structure of EAEC and how this pathotype evolved. Analysis by Multi Locus Sequence Typing of 564 EAEC isolates from cases and controls in Bangladesh, Nigeria and the UK spanning the past 29 years, revealed multiple successful lineages of EAEC. The population structure of EAEC indicates some clusters are statistically associated with disease or carriage, further highlighting the heterogeneous nature of this group of organisms. Different clusters have evolved independently as a result of both mutational and recombination events; the EAEC phenotype is distributed throughout the population of E. coli.
authors.affiliation: Gastrointestinal Bacteria Reference Unit, Public Health England, London, United Kingdom.
authors.forename: Marie Anne
authors.initials: MA
authors.lastname: Chattaway
authors.affiliation: Gastrointestinal Bacteria Reference Unit, Public Health England, London, United Kingdom.
authors.forename: Claire
authors.initials: C
authors.lastname: Jenkins
authors.affiliation: Genomic Service Unit, Public Health England, London, United Kingdom.
authors.forename: Dunstan
authors.initials: D
authors.lastname: Rajendram
authors.affiliation: International Vaccine Institute, Gwanak-gu, Seoul, Republic of Korea; Centre for Food and Water Borne Diseases, International Centre for Diarrhoeal Disease Research, Dhaka, Bangladesh.
authors.forename: Alejandro
authors.initials: A
authors.lastname: Cravioto
authors.affiliation: International Vaccine Institute, Gwanak-gu, Seoul, Republic of Korea.
authors.forename: Kaisar Ali
authors.initials: KA
authors.lastname: Talukder
authors.affiliation: Gastrointestinal Bacteria Reference Unit, Public Health England, London, United Kingdom.
authors.forename: Tim
authors.initials: T
authors.lastname: Dallman
authors.affiliation: Bioinformatics, PHE, London, United Kingdom.
authors.forename: Anthony
authors.initials: A
authors.lastname: Underwood
authors.affiliation: Bioinformatics, PHE, London, United Kingdom.
authors.forename: Steve
authors.initials: S
authors.lastname: Platt
authors.affiliation: Haverford College, Haverford, Pennsylvania, United States of America.
authors.forename: Iruka N
authors.initials: IN
authors.lastname: Okeke
authors.affiliation: Norwich Medical School, University of East Anglia, Norwich, United Kingdom.
authors.forename: John
authors.initials: J
authors.lastname: Wain
mesh_terms: D001459:Bangladesh; D004926:Escherichia coli / Q000145:classification / Q000235:genetics* / Q000472:pathogenicity*; D004927:Escherichia coli Infections / Q000382:microbiology*; D029968:Escherichia coli Proteins / Q000235:genetics; D019143:Evolution, Molecular; D006801:Humans; D058885:Multilocus Sequence Typing / Q000379:methods*; D009154:Mutation; D009549:Nigeria; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D011995:Recombination, Genetic; D013045:Species Specificity; D006113:United Kingdom; D014774:Virulence / Q000235:genetics
chemical_list: D029968:Escherichia coli Proteins