title: A microsystem integrating photodegradable hydrogel microstructures and reconfigurable microfluidics for single-cell analysis and retrieval.
abstract: We developed a micropatterned photodegradable hydrogel array integrated with reconfigurable microfluidics to enable cell secretion analysis and cell retrieval at the single-cell level. The activity of protease molecules secreted from single cells was monitored using FRET peptides entrapped inside microfabricated compartments. Antibody-modified gel islands tethering cells to the surface could be degraded by UV exposure to release specific single cells of interest.
authors.affiliation: Department of Biomedical Engineering, University of California, Davis, USA. arevzin@ucdavis.edu.
authors.forename: Kyung Jin
authors.initials: KJ
authors.lastname: Son
authors.forename: Dong-Sik
authors.initials: DS
authors.lastname: Shin
authors.forename: Timothy
authors.initials: T
authors.lastname: Kwa
authors.forename: Jungmok
authors.initials: J
authors.lastname: You
authors.forename: Yandong
authors.initials: Y
authors.lastname: Gao
authors.forename: Alexander
authors.initials: A
authors.lastname: Revzin
mesh_terms: D000906:Antibodies / Q000276:immunology; D015704:CD4 Antigens / Q000276:immunology; D031541:Fluorescence Resonance Energy Transfer; D006801:Humans; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry*; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods*; D010316:Particle Size; D010455:Peptides / Q000737:chemistry; D055668:Photochemical Processes; D059010:Single-Cell Analysis / Q000295:instrumentation* / Q000379:methods*; D013499:Surface Properties; D020298:U937 Cells
chemical_list: D000906:Antibodies; D015704:CD4 Antigens; D010455:Peptides; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate