title: Simultaneous Determination of Nine Active Compounds of the Traditional Chinese Medicinal Prescription Shaoyao-Gancao-Tang and Analysis of the Relationship between Therapeutical Effect and Compatibility of Medicines.
abstract: A simple and sensitive HPLC-DAD detection method was established for the simultaneous determination of nine compounds including oxypaeoniflorin, albiflorin, paeoniflorin, benzoylpaeoniflorin, glycyrrhizic acid, liquiritin, isoliquiritin, liquiritigenin, and isoliquiritigenin in the Traditional Chinese Medicinal Prescription Shaoyao-Gancao-Tang (SGT) and we analyze the relationship between therapeutical effect and compatibility of medicines by using an Agilent extend-C18 column at a flow rate of 1 mL/min. The column temperature was maintained at 30°C and the detection wavelength was set at 230 nm for oxypaeoniflorin, albiflorin, paeoniflorin, benzoylpaeoniflorin, and glycyrrhizic acid; 276 nm for liquiritin and liquiritigenin; 360 nm for isoliquiritin and isoliquiritigenin. The total contents of the nine compounds in SGT varied from 4.65 to 20.06 mg/mL. The results of this study showed that the content of chemical compounds of Traditional Chinese Medicinal Prescription is mainly influenced by the dosage and compatibility of medicines and the therapeutical effect of Traditional Chinese Medicinal prescription is mainly influenced by the dosage and compatibility of medicines. The method could be suitable for quality control of SGT with bioactive multicompounds.
authors.affiliation: School of Traditional Chinese Materia Medica, Beijing University of Traditional Chinese Medicine, Beijing 100102, China.
authors.forename: Guangwei
authors.identifier: 0000-0003-3917-7906
authors.initials: G
authors.lastname: Zhu
authors.affiliation: School of Traditional Chinese Materia Medica, Beijing University of Traditional Chinese Medicine, Beijing 100102, China.
authors.forename: Guijun
authors.initials: G
authors.lastname: Zhang
authors.affiliation: School of Traditional Chinese Materia Medica, Beijing University of Traditional Chinese Medicine, Beijing 100102, China.
authors.forename: Meng
authors.initials: M
authors.lastname: Wang
authors.affiliation: School of Traditional Chinese Materia Medica, Beijing University of Traditional Chinese Medicine, Beijing 100102, China.
authors.forename: Jingjuan
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Pharmacy, Beijing Shijitan Hospital, Beijing 100038, China.
authors.forename: Weixin
authors.initials: W
authors.lastname: Zeng
authors.affiliation: School of Traditional Chinese Materia Medica, Beijing University of Traditional Chinese Medicine, Beijing 100102, China.
authors.forename: Xiaomei
authors.initials: X
authors.lastname: Gao