title: Effect of pH on the formation of lysosome-alginate beads for antimicrobial activity.
abstract: In this study, we developed lysosome-alginate beads for application as an oral drug delivery system (ODDS). The beads harboring lysosomes, which have antimicrobial activity, and various concentrations of alginate were characterized and optimized. For application as an ODDS, pH-dependent lysosome-alginate beads were generated, and the level of lysosome release was investigated by using antimicrobial tests. At low pH, lysosomes were not released from the lysosome-alginate beads; however, at neutral pH, similar to the pH in the intestine, lysosome release was confirmed, as determined by a high antimicrobial activity. This study shows the potential of such an ODDS for the in vivo treatment of infection with pathogens.
authors.affiliation: Graduate School of Semiconductor and Chemical Engineering, Chonbuk National University, Jeonju 561-756, Republic of Korea.
authors.forename: Hyun Jung
authors.initials: HJ
authors.lastname: Park
authors.forename: Jiho
authors.initials: J
authors.lastname: Min
authors.forename: Joo-Myung
authors.initials: JM
authors.lastname: Ahn
authors.forename: Sung-Jin
authors.initials: SJ
authors.lastname: Cho
authors.forename: Ji-Young
authors.initials: JY
authors.lastname: Ahn
authors.forename: Yang-Hoon
authors.initials: YH
authors.lastname: Kim
mesh_terms: D000284:Administration, Oral; D000464:Alginates / Q000008:administration & dosage*; D000900:Anti-Bacterial Agents / Q000008:administration & dosage* / Q000737:chemistry; D016503:Drug Delivery Systems / Q000379:methods*; D048168:Escherichia coli K12 / Q000187:drug effects; D020723:Glucuronic Acid / Q000008:administration & dosage; D006603:Hexuronic Acids / Q000008:administration & dosage; D006863:Hydrogen-Ion Concentration; D008247:Lysosomes / Q000378:metabolism*; D008863:Microspheres
chemical_list: D000464:Alginates; D000900:Anti-Bacterial Agents; D006603:Hexuronic Acids; D020723:Glucuronic Acid