title: Role of IFN-γ and IL-6 cytokines and their association in determining susceptibility to chronic idiopathic urticaria.
abstract: Chronic Idiopathic Urticaria (CIU) is a common skin disorder, which may occur spontaneously. The aim of the present study was to assess the serum levels of interferon (IFN)-γ and interleukin (IL)-6 and to examine the association of IFN-γ+874 T/A and IL-6-174 G/C cytokine gene polymorphisms. To accomplish this, ELISA-based cytokine serum levels of IFN-γ (n=30) and IL-6 (n=30) in CIU patients (n=100) and Healthy Controls (HC) (n=200) were performed. Amplification refractory mutation system-polymerase chain reaction (ARMS-PCR) was performed to verify the positional significance. A significant (p<0.0001) increase in the serum cytokine levels of IFN-γ and IL-6 was recorded in CIU patients compared to HC. The AT and TT genotypes of IFN-γ and GG genotype of IL-6 were found to be significantly associated with CIU. In conclusion, our findings show a significant increase in the cytokine levels of IFN-γ and IL-6, highlighting their regulatory role in the development of disease. In addition to this, association studies have revealed that TT genotype of IFN-γ +874 T/A and GG genotype of IL-6-174 G/C were susceptible towards the CIU.
authors.affiliation: 1 Immunology Department, Bhagwan Mahavir Medical Research Centre , Hyderabad, Andhra Pradesh, India .
authors.forename: Madhavi Latha
authors.initials: ML
authors.lastname: Alasandagutti
authors.forename: Meenakshi
authors.initials: M
authors.lastname: Ponnana
authors.forename: Ramya
authors.initials: R
authors.lastname: Sivangala
authors.forename: Shruthi
authors.initials: S
authors.lastname: Thada
authors.forename: Lavanya
authors.initials: L
authors.lastname: Joshi
authors.forename: Hidayath
authors.initials: H
authors.lastname: Hussain
authors.forename: Soheb Sadath
authors.initials: SS
authors.lastname: Ansari
authors.forename: Vijayalakshmi
authors.initials: V
authors.lastname: Valluri
authors.forename: Suman Latha
authors.initials: SL
authors.lastname: Gaddam
mesh_terms: D000328:Adult; D002908:Chronic Disease; D005260:Female; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D007371:Interferon-gamma / Q000097:blood / Q000235:genetics; D015850:Interleukin-6 / Q000097:blood / Q000235:genetics; D008297:Male; D014581:Urticaria / Q000097:blood* / Q000235:genetics* / Q000473:pathology
chemical_list: C573415:IFNG protein, human; C508600:IL6 protein, human; D015850:Interleukin-6; D007371:Interferon-gamma