title: Selective, highly sensitive fluorescent probe for the detection of sulfur dioxide derivatives in aqueous and biological environments.
abstract: On the basis of a unique nucleophilic addition reaction, a novel water-soluble broadly emitting (500-700 nm) fluorescent Probe 1 was developed for the rapid detection of SO2 derivatives in aqueous media. The positively-charged benzopyrylium moiety in Probe 1 provides both excellent water solubility, making this probe applicable in 100% aqueous environments, and the ability to function as a fluorescence quencher of the coumarin moiety. Probe 1 generates a nearly instantaneous strong fluorescence signal in response to SO2 derivatives having an 8.3 nM detection limit for bisufite. The resulting Probe 1-sulfite adduct emits in the green/red spectral region (λ(max) = 585 nm) with a large Stokes shift (139 nm). The probe exhibits excellent selectivity toward SO2 derivatives over other potential interfering agents including reactive sulfur-containing species. Importantly, we demonstrate that Probe 1 can be used for the real-time sensing and bioimaging of SO2 derivatives in living cells.
authors.affiliation: College of Chemistry and Chemical Engineering, Central South University , Changsha, Hunan Province 410083, P. R. China.
authors.forename: Wenqiang
authors.initials: W
authors.lastname: Chen
authors.forename: Qian
authors.initials: Q
authors.lastname: Fang
authors.forename: Dalei
authors.initials: D
authors.lastname: Yang
authors.forename: Hongyan
authors.initials: H
authors.lastname: Zhang
authors.forename: Xiangzhi
authors.initials: X
authors.lastname: Song
authors.forename: James
authors.initials: J
authors.lastname: Foley
mesh_terms: D002294:Carcinoma, Squamous Cell / Q000378:metabolism* / Q000473:pathology; D049109:Cell Proliferation; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000138:chemical synthesis; D056945:Hep G2 Cells; D006801:Humans; D057230:Limit of Detection; D013458:Sulfur Dioxide / Q000032:analysis*; D014407:Tumor Cells, Cultured; D014867:Water / Q000737:chemistry
chemical_list: D005456:Fluorescent Dyes; D014867:Water; D013458:Sulfur Dioxide