title: Haematopoiesis radioprotection in Balb/c mice by an aqueous mycelium extract from the Basidiomycete Pleurotus ostreatus mushroom.
abstract: The study examined the radioprotective activity of an aqueous extract from Pleurotus ostreatus mycelium administered to Balb/c mice. Male mice were whole-body irradiated on day 0 ((60)Co, at 0.43 Gy/min) and divided into two groups. The extract was administered intraperitoneally to one group (100 mg/kg) on days - 10 to - 6 and - 2 to +1 with respect to the irradiation. The irradiated-control group was injected with saline solution; non-irradiated mice were used as negative controls. The radioprotective effect was evident by increases in bone marrow cellularity (5.1 × 10(6)/femur vs. 1.1 × 10(6)/femur in saline-control mice, p < 0.05), leucocyte counts (10.5 × 10(9)/L vs. 4.5 × 10(9)/L, p < 0.05), and spleen cellularity (11.2 × 10(7)/spleen vs. 6.2 × 10(7)/spleen, p < 0.05). The extract stimulated macrophage phagocytic activity as judged by a faster rate of carbon clearance in terms of absorbance ratios (1.62 vs. 2.01, p < 0.05). Therefore, this extract may be a candidate therapeutic agent with radioprotective activity for haematopoiesis damage, particularly to cells involved in immune function.
authors.affiliation: a Centre of Studies for Industrial Biotechnology (CEBI), Universidad de Oriente , Ave. Patricio Lumumba s/n, Reparto Jiménez, Santiago de Cuba 5 CP 90 500 , Cuba.
authors.forename: G
authors.initials: G
authors.lastname: Llauradó
authors.forename: H J
authors.initials: HJ
authors.lastname: Morris
authors.forename: V
authors.initials: V
authors.lastname: Tamayo
authors.forename: Y
authors.initials: Y
authors.lastname: Lebeque
authors.forename: Y
authors.initials: Y
authors.lastname: Beltrán
authors.forename: J
authors.initials: J
authors.lastname: Marcos
authors.forename: S
authors.initials: S
authors.lastname: Moukha
authors.forename: E E
authors.initials: EE
authors.lastname: Creppy
authors.forename: R C
authors.initials: RC
authors.lastname: Bermúdez
mesh_terms: D000363:Agaricales / Q000737:chemistry; D000818:Animals; D001853:Bone Marrow / Q000187:drug effects / Q000528:radiation effects; D006410:Hematopoiesis / Q000187:drug effects*; D007958:Leukocyte Count; D008264:Macrophages / Q000187:drug effects; D008297:Male; D008807:Mice, Inbred BALB C; D025282:Mycelium / Q000737:chemistry; D010587:Phagocytosis; D020076:Pleurotus / Q000737:chemistry*; D011837:Radiation-Protective Agents / Q000737:chemistry*; D013154:Spleen / Q000187:drug effects / Q000528:radiation effects
chemical_list: D011837:Radiation-Protective Agents
keywords: Pleurotus; haematopoiesis; immunomodulation; mushroom; radioprotection