title: Production of Retinal Cells from Confluent Human iPS Cells.
abstract: Human induced pluripotent stem (hiPS) cells could be used as an unlimited source of retinal cells for the treatment of retinal degenerative diseases. Although much progress has been made in the differentiation of pluripotent stem cells towards different retinal lineages, the production of retinal cells from hiPS cells for therapeutic approaches require the development of easy and standardized protocols. In this chapter, we describe a simple and effective protocol for retinal differentiation of hiPS cells bypassing embryoid body formation and the use of exogenous molecules and substrates. In 2 weeks, confluent hiPS cells cultured in pro-neural medium can generate both retinal pigmented epithelial cells and self-forming neural retina-like structures containing retinal progenitor cells. These progenitors can be differentiated into all retinal cell types, including retinal ganglion cells and precursors of photoreceptors, which could find important applications in regenerative medicine. This differentiation system and the resulting hiPS-derived retinal cells will also offer opportunity to study the molecular and cellular mechanisms underlying human retinal development, and the establishment of in vitro models of human retinal degenerative diseases.
authors.affiliation: Institut de la Vision, Institut National de la Santé et de la Recherche Médicale, U968 , 17 Rue Moreau, 75012, Paris, France.|Université Pierre-et-Marie-Curie Paris 6, Sorbonne Universités, UMR S968 , Paris, France.|Centre National de la Recherche Scientifique, UMR 7210, Paris, France.
authors.forename: Sacha
authors.initials: S
authors.lastname: Reichman
authors.affiliation: Institut de la Vision, Institut National de la Santé et de la Recherche Médicale, U968 , 17 Rue Moreau, 75012, Paris, France. olivier.goureau@inserm.fr.|Université Pierre-et-Marie-Curie Paris 6, Sorbonne Universités, UMR S968 , Paris, France. olivier.goureau@inserm.fr.|Centre National de la Recherche Scientifique, UMR 7210, Paris, France. olivier.goureau@inserm.fr.
authors.forename: Olivier
authors.initials: O
authors.lastname: Goureau
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation; D002478:Cells, Cultured; D065150:Cellular Reprogramming; D000067470:Cellular Reprogramming Techniques / Q000379:methods*; D016895:Culture Media, Serum-Free; D005347:Fibroblasts / Q000166:cytology; D015870:Gene Expression; D005822:Genetic Vectors / Q000235:genetics; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D057026:Induced Pluripotent Stem Cells / Q000166:cytology*; D051379:Mice; D010957:Plasmids / Q000235:genetics; D012160:Retina / Q000166:cytology*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods
chemical_list: D016895:Culture Media, Serum-Free
keywords: Eye field; Glaucoma; Human iPS cells; Photoreceptor; Retinal ganglion cell; Retinal pigmented epithelium; Retinal progenitor; Retinopathies; Three-dimensional retina