title: First experience of a multicenter external quality assessment of molecular 16S rRNA gene detection in bone and joint infections.
abstract: The objective of this study was to assess the performance of seven French laboratories for 16S rRNA gene detection by real-time PCR in the diagnosis of bone and joint infection (BJI) to validate a large multicenter study. External quality control (QC) was required owing to the differences in extraction procedures and the molecular equipment used in the different laboratories. Three proficiency sets were organized, including four bacterial DNA extracts and four bead mill-pretreated osteoarticular specimens. Extraction volumes, 16S rRNA gene primers, and sequencing interpretation rules were standardized. In order to assess each laboratory's ability to achieve the best results, scores were assigned, and each QC series was classified as optimal, acceptable, or to be improved. A total of 168 QCs were sent, and 160 responses were analyzed. The expected results were obtained for 93.8%, with the same proportion for extracts (75/80) and clinical specimens (75/80). For the specimens, there was no significant difference between manual and automated extraction. This QC demonstrated the ability to achieve good and homogeneous results using the same 16S rRNA gene PCR with different equipment and validates the possibility of high-quality multicenter studies using molecular diagnosis for BJI.
authors.affiliation: CHU Poitiers, Laboratoire de Bactériologie, Poitiers, France chloe.plouzeau-jayle@chu-poitiers.fr.
authors.forename: Chloé
authors.initials: C
authors.lastname: Plouzeau
authors.affiliation: CHU Nantes, Laboratoire de Bactériologie, Nantes, France.
authors.forename: Pascale
authors.initials: P
authors.lastname: Bémer
authors.affiliation: CHU Tours Laboratoire de Bactériologie, Tours, France.
authors.forename: Anne Sophie
authors.initials: AS
authors.lastname: Valentin
authors.affiliation: CHU Brest, Laboratoire de Bactériologie, Brest, France.
authors.forename: Geneviève
authors.initials: G
authors.lastname: Héry-Arnaud
authors.affiliation: CHU Brest, Laboratoire de Bactériologie, Brest, France.
authors.forename: Didier
authors.initials: D
authors.lastname: Tandé
authors.affiliation: CHU Rennes, Laboratoire de Bactériologie, Rennes, France.
authors.forename: Anne
authors.initials: A
authors.lastname: Jolivet-Gougeon
authors.affiliation: CHU Rennes, Laboratoire de Bactériologie, Rennes, France.
authors.forename: Pascal
authors.initials: P
authors.lastname: Vincent
authors.affiliation: CHU Angers Laboratoire de Bactériologie, Angers, France.
authors.forename: Marie
authors.initials: M
authors.lastname: Kempf
authors.affiliation: CHU Angers Laboratoire de Bactériologie, Angers, France.
authors.forename: Carole
authors.initials: C
authors.lastname: Lemarié
authors.affiliation: CH Orléans, Laboratoire de Bactériologie, Orléans, France.
authors.forename: Jérôme
authors.initials: J
authors.lastname: Guinard
authors.affiliation: CH Orléans, Laboratoire de Bactériologie, Orléans, France.
authors.forename: Laurent
authors.initials: L
authors.lastname: Bret
authors.affiliation: CHU Poitiers, Laboratoire de Bactériologie, Poitiers, France.
authors.forename: Anne Sophie
authors.initials: AS
authors.lastname: Cognée
authors.affiliation: CHU Nantes, Laboratoire de Bactériologie, Nantes, France.
authors.forename: Sophie
authors.initials: S
authors.lastname: Gibaud
authors.affiliation: CHU Poitiers, Laboratoire de Bactériologie, Poitiers, France.
authors.forename: Christophe
authors.initials: C
authors.lastname: Burucoa
authors.affiliation: CHU Nantes, Laboratoire de Bactériologie, Nantes, France.
authors.forename: Stéphane
authors.initials: S
authors.lastname: Corvec
mesh_terms: D001424:Bacterial Infections / Q000175:diagnosis* / Q000382:microbiology*; D004275:DNA, Ribosomal / Q000235:genetics; D005602:France; D020459:Genes, rRNA; D006801:Humans; D059021:Laboratory Proficiency Testing; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D010003:Osteoarthritis / Q000175:diagnosis* / Q000382:microbiology*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*
chemical_list: D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S