title: Ankyrin is the major oxidised protein in erythrocyte membranes from end-stage renal disease patients on chronic haemodialysis and oxidation is decreased by dialysis and vitamin C supplementation.
abstract: Chronically haemodialysed end-stage renal disease patients are at high risk of morbidity arising from complications of dialysis, the underlying pathology that has led to renal disease and the complex pathology of chronic kidney disease. Anaemia is commonplace and its origins are multifactorial, involving reduced renal erythropoietin production, accumulation of uremic toxins and an increase in erythrocyte fragility. Oxidative damage is a common risk factor in renal disease and its co-morbidities and is known to cause erythrocyte fragility. Therefore, we have investigated the hypothesis that specific erythrocyte membrane proteins are more oxidised in end-stage renal disease patients and that vitamin C supplementation can ameliorate membrane protein oxidation. Eleven patients and 15 control subjects were recruited to the study. Patients were supplemented with 2 × 500 mg vitamin C per day for 4 weeks. Erythrocyte membrane proteins were prepared pre- and post-vitamin C supplementation for determination of protein oxidation. Total protein carbonyls were reduced by vitamin C supplementation but not by dialysis when investigated by enzyme linked immunosorbent assay. Using a western blot to detect oxidised proteins, one protein band, later identified as containing ankyrin, was found to be oxidised in patients but not controls and was reduced significantly by 60% in all patients after dialysis and by 20% after vitamin C treatment pre-dialysis. Ankyrin oxidation analysis may be useful in a stratified medicines approach as a possible marker to identify requirements for intervention in dialysis patients.
authors.affiliation: Faculty of Medical Sciences, GoceDelcev University , Stip , Republic of Macedonia.
authors.forename: T
authors.initials: T
authors.lastname: Ruskovska
authors.forename: S J
authors.initials: SJ
authors.lastname: Bennett
authors.forename: C R
authors.initials: CR
authors.lastname: Brown
authors.forename: S
authors.initials: S
authors.lastname: Dimitrov
authors.forename: N
authors.initials: N
authors.lastname: Kamcev
authors.forename: H R
authors.initials: HR
authors.lastname: Griffiths
mesh_terms: D017487:Ankyrins / Q000737:chemistry* / Q000187:drug effects; D001205:Ascorbic Acid / Q000627:therapeutic use*; D004910:Erythrocyte Membrane / Q000737:chemistry* / Q000187:drug effects; D006801:Humans; D007676:Kidney Failure, Chronic / Q000097:blood* / Q000188:drug therapy / Q000628:therapy; D010084:Oxidation-Reduction / Q000187:drug effects; D006435:Renal Dialysis
chemical_list: D017487:Ankyrins; D001205:Ascorbic Acid
keywords: carbonyl; protein oxidation; red blood cells