title: Evaluation of small intestine submucosa and poly(caprolactone-co-lactide) conduits for peripheral nerve regeneration.
abstract: The present study employed nerve guidance conduits (NGCs) only, which were made of small intestine submucosa (SIS) and poly(caprolactone-co-lactide) (PCLA) to promote nerve regeneration in a peripheral nerve injury (PNI) model with nerve defects of 15 mm. The SIS- and PCLA-NGCs were easily prepared by rolling of a SIS sheet and a bioplotter using PCLA, respectively. The prepared SIS- and PCLA-NGCs fulfilled the general requirement for use as artificial peripheral NGCs such as easy fabrication, reproducibility for mass production, suturability, sterilizability, wettability, and proper mechanical properties to resist collapsing when applied to in vivo implantation. The SIS- and PCLA-NGCs appeared to be well integrated into the host sciatic nerve without causing dislocations and serious inflammation. All NGCs stably maintained their NGC shape for 8 weeks without collapsing, which matched well with the nerve regeneration rate. Staining of the NGCs in the longitudinal direction showed that the regenerated nerves grew successfully from the SIS- and PCLA-NGCs through the sciatic nerve-injured gap and connected from the proximal to distal direction along the NGC axis. SIS-NGCs exhibited a higher nerve regeneration rate than PCLA-NGCs. Collectively, our results indicate that SIS- and PCLA-NGCs induced nerve regeneration in a PNI model, a finding that has significant implications in the future with regard to the feasibility of clinical nerve regeneration with SIS- and PCLA-NGCs prepared through an easy fabrication method using promising biomaterials.
authors.affiliation: 1 Department of Molecular Science and Technology, Ajou University , Suwon, Korea.
authors.forename: Sun Woo
authors.initials: SW
authors.lastname: Shim
authors.forename: Doo Yeon
authors.initials: DY
authors.lastname: Kwon
authors.forename: Bit Na
authors.initials: BN
authors.lastname: Lee
authors.forename: Jin Seon
authors.initials: JS
authors.lastname: Kwon
authors.forename: Ji Hoon
authors.initials: JH
authors.lastname: Park
authors.forename: Jun Hee
authors.initials: JH
authors.lastname: Lee
authors.forename: Jae Ho
authors.initials: JH
authors.lastname: Kim
authors.forename: Il Woo
authors.initials: IW
authors.lastname: Lee
authors.forename: Jung-Woog
authors.initials: JW
authors.lastname: Shin
authors.forename: Hai Bang
authors.initials: HB
authors.lastname: Lee
authors.forename: Wan-Doo
authors.initials: WD
authors.lastname: Kim
authors.forename: Moon Suk
authors.initials: MS
authors.lastname: Kim
mesh_terms: D000818:Animals; D002452:Cell Count; D005260:Female; D048091:Guided Tissue Regeneration / Q000379:methods*; D007413:Intestinal Mucosa; D007421:Intestine, Small / Q000187:drug effects / Q000502:physiology*; D009416:Nerve Regeneration / Q000187:drug effects*; D011091:Polyesters / Q000494:pharmacology*; D019919:Prosthesis Implantation; D017207:Rats, Sprague-Dawley; D012584:Sciatic Nerve / Q000473:pathology / Q000503:physiopathology* / Q000601:surgery; D013194:Staining and Labeling; D034421:Sus scrofa
chemical_list: D011091:Polyesters; C553659:polycaprolactone-co-lactide