title: Application of a sensitive and specific LC-MS/MS method for determination of wilforine from Tripterygium wilfordii Hook. F. in rat plasma for a bioavailability study.
abstract: A highly selective and specific LC-MS/MS method was developed and validated for the determination of wilforine in rat plasma. The analyte was separated from plasma matrix by using methyl tertiary butyl ether liquid-liquid extraction with bulleyacinitine A as internal standard (IS). The analysis was carried out on a Sepax GP-Phenyl column using a mixture of methanol and 10 mmol/L ammonium formate buffer solution containing 0.1% formic acid (75:25, v/v) as the mobile phase pumped at a flow rate of 1.0 mL/min. The detection was operated using a triple-quadrupole mass spectrometer in multiple selected reaction monitoring with the parent-to-product quantifier transitions [M + H](+) m/z 867.6 →206.0 for wilforine and 664.1 →584.1 for IS. The main advantage of this method was the high sensitivity (a lower limit of quantification of 0.02 ng/mL) and the small amount of sample (0.1 mL plasma per sample). The method was fully validated to be accurate and precise with a linear range of 0.02-100 ng/mL, and successfully applied to a bioavailability study of wilforine in rats after intravenous and oral administration. The oral absolute bioavailability of wilforine in rats was estimated to be 84%.
authors.affiliation: Department of Pharmaceutical Analysis, China Pharmaceutical University, Nanjing, 210009, China.|Key Laboratory of Drug Quality Control and Pharmacovigilance (China Pharmaceutical University), Ministry of Education, Nanjing, 210009, China.
authors.forename: Meng-Xiang
authors.initials: MX
authors.lastname: Su
authors.affiliation: Department of Pharmaceutical Analysis, China Pharmaceutical University, Nanjing, 210009, China.|Key Laboratory of Drug Quality Control and Pharmacovigilance (China Pharmaceutical University), Ministry of Education, Nanjing, 210009, China.
authors.forename: Min
authors.initials: M
authors.lastname: Song
authors.affiliation: Department of Pharmaceutical Analysis, China Pharmaceutical University, Nanjing, 210009, China.|Key Laboratory of Drug Quality Control and Pharmacovigilance (China Pharmaceutical University), Ministry of Education, Nanjing, 210009, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Di
authors.affiliation: Department of Pharmaceutical Analysis, China Pharmaceutical University, Nanjing, 210009, China.|Key Laboratory of Drug Quality Control and Pharmacovigilance (China Pharmaceutical University), Ministry of Education, Nanjing, 210009, China.
authors.forename: Tai-jun
authors.initials: TJ
authors.lastname: Hang
mesh_terms: D000818:Animals; D001682:Biological Availability; D002853:Chromatography, Liquid / Q000379:methods*; D004355:Drug Stability; D007783:Lactones / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics; D016014:Linear Models; D011725:Pyridines / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics; D051381:Rats; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods*; D029757:Tripterygium
chemical_list: D007783:Lactones; D011725:Pyridines; C535115:wilforine
keywords: LC-MS/MS; Tripterygium wilfordii; bioequivalence; rat; wilforine