title: Stem cell biology in thyroid cancer: Insights for novel therapies.
abstract: Currently, thyroid cancer is one of the most common endocrine cancer in the United States. A recent involvement of sub-population of stem cells, cancer stem cells, has been proposed in different histological types of thyroid cancer. Because of their ability of self-renewal and differentiation into various specialized cells in the body, these putative cells drive tumor genesis, metastatic activity and are responsible to provide chemo- and radioresistant nature to the cancer cells in the thyroid gland. Our Review was conducted from previously published literature to provide latest apprises to investigate the role of embryonic, somatic and cancer stem cells, and discusses the hypothesis of epithelial-mesenchymal transition. Different methods for their identification and isolation through stemness markers using various in vivo and in vitro methods such as flow cytometry, thyrosphere formation assay, aldehyde dehydrogenase activity and ATP-binding cassette sub-family G member 2 efflux-pump mediated Hoechst 33342 dye exclusion have been discussed. The review also outlines various setbacks that still remain to target these tumor initiating cells. Future perspectives of therapeutic strategies and their potential to treat advanced stages of thyroid cancer are also disclosed in this review.
authors.affiliation: Parisha Bhatia, Koji Tsumagari, Zakaria Y Abd Elmageed, Joseph F Buell, Emad Kandil, Departments of Surgery, Tulane University School of Medicine, New Orleans, LA 70112, United States.
authors.forename: Parisha
authors.initials: P
authors.lastname: Bhatia
authors.affiliation: Parisha Bhatia, Koji Tsumagari, Zakaria Y Abd Elmageed, Joseph F Buell, Emad Kandil, Departments of Surgery, Tulane University School of Medicine, New Orleans, LA 70112, United States.
authors.forename: Koji
authors.initials: K
authors.lastname: Tsumagari
authors.affiliation: Parisha Bhatia, Koji Tsumagari, Zakaria Y Abd Elmageed, Joseph F Buell, Emad Kandil, Departments of Surgery, Tulane University School of Medicine, New Orleans, LA 70112, United States.
authors.forename: Zakaria Y
authors.initials: ZY
authors.lastname: Abd Elmageed
authors.affiliation: Parisha Bhatia, Koji Tsumagari, Zakaria Y Abd Elmageed, Joseph F Buell, Emad Kandil, Departments of Surgery, Tulane University School of Medicine, New Orleans, LA 70112, United States.
authors.forename: Paul
authors.initials: P
authors.lastname: Friedlander
authors.affiliation: Parisha Bhatia, Koji Tsumagari, Zakaria Y Abd Elmageed, Joseph F Buell, Emad Kandil, Departments of Surgery, Tulane University School of Medicine, New Orleans, LA 70112, United States.
authors.forename: Joseph F
authors.initials: JF
authors.lastname: Buell
authors.affiliation: Parisha Bhatia, Koji Tsumagari, Zakaria Y Abd Elmageed, Joseph F Buell, Emad Kandil, Departments of Surgery, Tulane University School of Medicine, New Orleans, LA 70112, United States.
authors.forename: Emad
authors.initials: E
authors.lastname: Kandil
keywords: Cancer Stem cells; Carcinogenesis; Cell Differentiation; Epithelial-Mesenchymal transition; Stem cells; Thyroid Neoplasms