title: Huperzine A production by Paecilomyces tenuis YS-13, an endophytic fungus isolated from Huperzia serrata.
abstract: Huperzine A (HupA), a naturally occurring alkaloid in the plant family Huperziaceae, has drawn great interest for its potential application in Alzheimer disease therapy. Our primary objective was to identify alkaloid- and HupA-producing fungi from the Chinese folk herb, Huperzia serrata. We established a rapid and efficient model for screening HupA-producing endophytic fungal strains. The presence of HupA in Paecilomyces tenuis YS-13 was analysed by thin-layer chromatography, high-performance liquid chromatography and mass spectrometry. The fermentation yield of HupA was 21.0 μg/L, and the IC50 of the crude extract of YS-13 fermentation broth was 1.27 ± 0.04 mg/mL. This is the first report of P. tenuis as a HupA-producing endophyte isolated from Huperziaceae.
authors.affiliation: a College of Life Science , Fujian Normal University , Fuzhou 350108 , P.R. China.
authors.forename: Jingqian
authors.initials: J
authors.lastname: Su
authors.forename: Minhe
authors.initials: M
authors.lastname: Yang
mesh_terms: D000470:Alkaloids / Q000096:biosynthesis / Q000737:chemistry*; D002800:Cholinesterase Inhibitors / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D060026:Endophytes / Q000737:chemistry; D005285:Fermentation; D032501:Huperzia / Q000382:microbiology*; D013058:Mass Spectrometry; D015394:Molecular Structure; D010143:Paecilomyces / Q000737:chemistry*; D012717:Sesquiterpenes / Q000737:chemistry*
chemical_list: D000470:Alkaloids; D002800:Cholinesterase Inhibitors; D012717:Sesquiterpenes; C050426:huperzine A
keywords: Alzheimer disease; Huperzia serrata; Paecilomyces tenuis; endophytic fungus; huperzine A