title: Antibacterial and hemolytic activity of a new lectin purified from the seeds of Sterculia foetida L.
abstract: The aim of this study was to isolate, characterize, and verify possible antibacterial and hemolytic activity for a lectin found in the seeds of Sterculia foetida L. Purification of the lectin from S. foetida (SFL) was realized with ion exchange chromatography DEAE-Sephacel coupled to HPLC. The purity and the molecular weight was determined by SDS-PAGE. The isolated SFL was characterized as to its glycoprotein nature, and sugar specificity, as well as resistance to pH, temperature, denaturing agents, reduction, oxidation, and chelation. A microdilution method was used to determine antibacterial activity, and hemolytic activity was observed in human erythrocytes. The SFL has a molecular weight of 17 kDa, and a carbohydrate content of 53 μg/mL, specific for arabinose and xylose, and is resistant to treatment with urea, sensitive to treatment with sodium metaperiodate and β-mercaptoethanol, and in the presence of EDTA lost its hemagglutinating activity (HA). However, in the presence of divalent cations (Ca(2 +) and Mn(2 +)) the HA was increased. The SFL remained active even after incubation at 80 °C, and, within pH values of between 5 and 11. The SFL inhibited the bacterial growth of all the tested strains and caused little hemolysis in human erythrocytes when compared to the positive control Triton X-100.
authors.affiliation: Department of Molecular Biology, Federal University of Paraíba, Campus I, João Pessoa, Paraíba, CEP 58059-900, Brazil, alanaaraujobraga@gmail.com.
authors.forename: Alana Araújo
authors.initials: AA
authors.lastname: Braga
authors.forename: Rodrigo
authors.initials: R
authors.lastname: Rodrigues e Lacerda
authors.forename: Gracy Kelly Vieira de Vasconcelos
authors.initials: GK
authors.lastname: Medeiros
authors.forename: Gregório Fernandes
authors.initials: GF
authors.lastname: Gonçalves
authors.forename: Hilzeth de Luna Freire
authors.initials: Hde L
authors.lastname: Pessoa
authors.forename: Juscélio Donizete
authors.initials: JD
authors.lastname: Cardoso
authors.forename: Carlos Alberto de Almeida
authors.initials: CA
authors.lastname: Gadelha
authors.forename: Bagnólia Araújo
authors.initials: BA
authors.lastname: da Silva
authors.forename: Tatiane
authors.initials: T
authors.lastname: Santi-Gadelha
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D004912:Erythrocytes / Q000187:drug effects; D006461:Hemolysis / Q000187:drug effects*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008297:Male; D008826:Microbial Sensitivity Tests; D037121:Plant Lectins / Q000302:isolation & purification* / Q000494:pharmacology*; D011817:Rabbits; D012639:Seeds / Q000737:chemistry*; D032361:Sterculia / Q000737:chemistry*; D013696:Temperature
chemical_list: D000900:Anti-Bacterial Agents; D037121:Plant Lectins