title: Multicopy integration and expression of heterologous genes in the oleaginous yeast, Lipomyces starkeyi.
abstract: The oleaginous yeast, Lipomyces starkeyi, is an excellent lipid producer with great industrial potential. However, methods for molecular breeding have not been established for L. starkeyi. We describe the development of a system for targeted rDNA integration of multiple copies of a gene into L. starkeyi genome by spheroplast-polyethylene glycol transformation.
authors.affiliation: a Department of Applied Life Sciences , Niigata University of Pharmacy and Applied Life Sciences , Niigata , Japan.
authors.forename: Yoshifumi
authors.initials: Y
authors.lastname: Oguro
authors.forename: Harutake
authors.initials: H
authors.lastname: Yamazaki
authors.forename: Yosuke
authors.initials: Y
authors.lastname: Shida
authors.forename: Wataru
authors.initials: W
authors.lastname: Ogasawara
authors.forename: Masamichi
authors.initials: M
authors.lastname: Takagi
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Takaku
mesh_terms: D004275:DNA, Ribosomal / Q000235:genetics; D015870:Gene Expression; D005818:Genetic Engineering / Q000379:methods*; D016681:Genome, Fungal / Q000235:genetics; D055160:Lipomyces / Q000235:genetics*; D011092:Polyethylene Glycols / Q000737:chemistry; D014170:Transformation, Genetic
chemical_list: D004275:DNA, Ribosomal; D011092:Polyethylene Glycols
keywords: Lipomyces starkeyi; multicopy integration; oleaginous yeast; transformation