title: Isolation of filamentous fungi exhibiting high endoxylanase activity in lignocellulose hydrolysate.
abstract: For complete degradation of hemicellulose into its monomers from lignocellulose biomass, the synergistic action of a broad range of hydrolytic enzymes is needed. Therefore, production of enzymes from their natural producer is desirable. To obtain a powerful β-1,4-endoxylanase producing fungus, 304 environmental samples were collected from various locations in Singapore, leading to 603 isolates. Among them, 71 exhibiting β-1,4-endoxylanase activity were identified belonging mainly to the genera of Aspergillus, Penicillium, and Trichoderma. Further analysis revealed Aspergillus niger DSM 26641 as a potential and stable β-1,4-endoxylanase producer, being able to grow in hydrothermal lignocellulose hydrolysate exhibiting its maximal β-1,4-endoxylanase activity at pH 4 and 60 °C. This strain is thought to be very suitable for lactic acid production in a simultaneous saccharification and fermentation at pH values below 5.
authors.affiliation: Microbiology and Bioprocess Engineering, Institute of Biochemistry, Leipzig University, 04103, Leipzig, Germany, christoph@ottenheim.net.
authors.forename: Christoph
authors.initials: C
authors.lastname: Ottenheim
authors.forename: Kirstin
authors.initials: K
authors.lastname: Meier
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Zimmermann
authors.forename: Jin Chuan
authors.initials: JC
authors.lastname: Wu
mesh_terms: D001234:Aspergillus niger / Q000145:classification / Q000201:enzymology / Q000235:genetics / Q000302:isolation & purification*; D018533:Biomass; D004271:DNA, Fungal / Q000235:genetics; D043364:Endo-1,4-beta Xylanases / Q000235:genetics / Q000378:metabolism*; D005285:Fermentation; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism*; D015870:Gene Expression; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D019344:Lactic Acid / Q000096:biosynthesis; D008031:Lignin / Q000378:metabolism*; D010407:Penicillium / Q000145:classification / Q000201:enzymology / Q000235:genetics / Q000302:isolation & purification*; D010802:Phylogeny; D011134:Polysaccharides / Q000378:metabolism*; D012846:Singapore; D014242:Trichoderma / Q000145:classification / Q000201:enzymology / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D004271:DNA, Fungal; D005656:Fungal Proteins; D011134:Polysaccharides; C036909:lignocellulose; D019344:Lactic Acid; C007916:hemicellulose; D008031:Lignin; D043364:Endo-1,4-beta Xylanases