title: Multiple turnovers of DNAzyme for amplified detection of ATP and reduced thiol in cell homogenates.
abstract: A conveniently amplified DNAzyme-based fluorescence strategy was designed for highly sensitive detection of ATP or reduced thiol based on the introduction of an ATP aptamer or a disulfide bond in the bioconjugates of magnetic nanoparticles (MNP) and polystyrene microsphere-DNAzyme complexes (PSM-DNAzyme).
authors.affiliation: State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, China. xujj@nju.edu.cn.
authors.forename: Yingshu
authors.initials: Y
authors.lastname: Guo
authors.forename: Jia
authors.initials: J
authors.lastname: Liu
authors.forename: Guangxu
authors.initials: G
authors.lastname: Yang
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Sun
authors.forename: Hong-Yuan
authors.initials: HY
authors.lastname: Chen
authors.forename: Jing-Juan
authors.initials: JJ
authors.lastname: Xu
mesh_terms: D000255:Adenosine Triphosphate / Q000032:analysis* / Q000378:metabolism; D052157:Aptamers, Nucleotide / Q000378:metabolism; D001483:Base Sequence; D015374:Biosensing Techniques / Q000379:methods*; D021881:DNA, Catalytic / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D053758:Nanoparticles / Q000737:chemistry; D010084:Oxidation-Reduction; D011137:Polystyrenes / Q000737:chemistry; D013050:Spectrometry, Fluorescence; D013438:Sulfhydryl Compounds / Q000032:analysis* / Q000378:metabolism*
chemical_list: D052157:Aptamers, Nucleotide; D021881:DNA, Catalytic; D011137:Polystyrenes; D013438:Sulfhydryl Compounds; D000255:Adenosine Triphosphate