title: Serum prolidase activity and oxidant-antioxidant status in children with chronic hepatitis B virus infection.
abstract: BACKGROUND
  Chronic hepatitis B (CHB) is a global health problem that can result in serious complications associated with collagen degradation. Prolidase is a specific imidodipeptidase that plays an important role in the breakdown of collagen. The aim of this study was to investigate prolidase activity and oxidant-antioxidant status in children with CHB.
  
  
  METHODS
  This prospective case control study includes 38 patients with CHB, 31 patients with inactive hepatitis B (IHB), and 29 healthy matched control subjects. Serum prolidase enzyme activity (SPEA), total antioxidant capacity (TAC), total oxidative activity (TOA), and malondialdehyde (MDA) level were measured and oxidative stress index (OSI) was calculated for each group.
  
  
  RESULTS
  Patients with CHB had significantly higher SPEA levels (207.82 ± 186.80 IU/L) than did the controls (58.6 ± 38.1 IU/L) and IHB patients (67.1 ± 39.9) (p < 0.001). CHB patients also had significantly higher TOA (45.0 ± 19.9 vs. 29.4 ± 11.7 (μmolH2O2 Eq./L), p = 0.005), OSI (33.1 ± 21.4 vs. 17.5 ± 10.2, p = 0.002) and MDA (13.4 ± 4.0 vs. 7.8 ± 2.6 μm/L, p < 0.001) values compared with the controls. TOA (32.0 ± 10.0) and OSI (15.4 ± 11.0) values of IHB patients were significantly lower than those of CHB patients (p < 0.05). SPEA had significant correlations with HBV- DNA and ALT values (r =0.514 and r =0.454, p < 0.001).
  
  
  CONCLUSIONS
  Our results suggest that prolidase activity can be considered as a reliable marker for CHB and increased oxidative stress appears to be related to chronicity of the disease.
authors.affiliation: Department of Pediatrics, Dicle University Medical School, Diyarbakir, Turkey. drvelatsen@hotmail.com.
authors.forename: Velat
authors.initials: V
authors.lastname: Şen
authors.affiliation: Department of Pediatrics, Dicle University Medical School, Diyarbakir, Turkey. draydinece@hotmail.com.
authors.forename: Ünal
authors.initials: Ü
authors.lastname: Uluca
authors.affiliation: Department of Pediatrics, Dicle University Medical School, Diyarbakir, Turkey. ulucau@gmail.com.
authors.forename: Aydın
authors.initials: A
authors.lastname: Ece
authors.affiliation: Department of Biochemistry, Dicle University Medical School, Diyarbakir, Turkey. ikaplan@gmail.com.
authors.forename: İbrahim
authors.initials: İ
authors.lastname: Kaplan
authors.affiliation: Department of Infectious Diseases and Clinical Microbiology, Dicle University Medical School, Diyarbakir, Turkey. drfatmayakut@hotmail.com.
authors.forename: Fatma
authors.initials: F
authors.lastname: Bozkurt
authors.affiliation: Department of Pediatrics, Dicle University Medical School, Diyarbakir, Turkey. fesihaktar@yahoo.com.
authors.forename: Fesih
authors.initials: F
authors.lastname: Aktar
authors.affiliation: Department of Pediatrics, Dicle University Medical School, Diyarbakir, Turkey. sedatbgl@gmail.com.
authors.forename: Sedat
authors.initials: S
authors.lastname: Bağlı
authors.affiliation: Department of Infectious Diseases and Clinical Microbiology, Dicle University Medical School, Diyarbakir, Turkey. receptekin@yahoo.com.
authors.forename: Recep
authors.initials: R
authors.lastname: Tekin
mesh_terms: D000293:Adolescent; D000975:Antioxidants / Q000378:metabolism*; D002648:Child; D002675:Child, Preschool; D004150:Dipeptidases / Q000097:blood*; D005260:Female; D019694:Hepatitis B, Chronic / Q000097:blood*; D006801:Humans; D008297:Male; D016877:Oxidants / Q000097:blood*; D018384:Oxidative Stress; D011446:Prospective Studies
chemical_list: D000975:Antioxidants; D016877:Oxidants; D004150:Dipeptidases; C022449:proline dipeptidase