title: Polysaccharides from Angelica and Astragalus exert hepatoprotective effects against carbon-tetrachloride-induced intoxication in mice.
abstract: This study aimed to investigate the effects of polysaccharide from Angelica and Astragalus (AAP) on carbon tetrachloride (CCl4) induced liver damage in mice. A total of 120 Kunming mice were randomly distributed among 6 groups comprising (i) the normal control mice, (ii) the CCl4 treatment group, (iii) the bifendate treatment group, (iv) the AAP treatment group, (v) the Angelica sinensis polysaccharide (ASP) treatment group, and (vi) the Astragalus membranaceus polysaccharide (AMP) treatment group. AAP, ASP and AMP were administered to mice treated with CCl4. The activities of alanine transaminase (ALT) and aspartate transaminase (AST) in the serum, and superoxide dismutase (SOD) and malondialdehyde (MDA) in the liver tissues were quantified, as well as the liver index. Hepatic histological changes were observed by staining liver sections with hematoxylin and eosin. Our results show that bifendate, AAP, ASP, and AMP significantly decreased the activities of MDA, AST, and ALT, and enhanced the activity of SOD in CCl4-treated mice. Bifendate, AAP, ASP, and AMP consistently ameliorated the liver injuries induced with CCl4. Notably, the hepatoprotective effect of AAP was stronger than that of bifendate, ASP, or AMP. In addition, AAP alleviated liver inflammation and decreased the liver indexes of mice induced with CCl4. These effects were at least partly due to the antioxidant properties of AAP in scavenging free radicals to ameliorate oxidative stress and to inhibit lipid peroxidation.
authors.affiliation: a College of Life Science and Engineering, Lanzhou University of Technology, No. 287 Langongping Street, Qilihe District, Lanzhou City 730050, Gansu Province, P.R. China.
authors.forename: Xiuying
authors.initials: X
authors.lastname: Pu
authors.forename: Wenbo
authors.initials: W
authors.lastname: Fan
authors.forename: Shuang
authors.initials: S
authors.lastname: Yu
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.forename: Xiaolong
authors.initials: X
authors.lastname: Ma
authors.forename: Lu
authors.initials: L
authors.lastname: Liu
authors.forename: Jing
authors.initials: J
authors.lastname: Ren
authors.forename: Weijie
authors.initials: W
authors.lastname: Zhang
mesh_terms: D029969:Angelica; D000818:Animals; D027883:Astragalus Plant; D002251:Carbon Tetrachloride / Q000633:toxicity*; D056486:Chemical and Drug Induced Liver Injury / Q000473:pathology / Q000517:prevention & control*; D005260:Female; D008297:Male; D051379:Mice; D010936:Plant Extracts / Q000302:isolation & purification / Q000627:therapeutic use*; D011134:Polysaccharides / Q000302:isolation & purification / Q000627:therapeutic use*
chemical_list: D010936:Plant Extracts; D011134:Polysaccharides; D002251:Carbon Tetrachloride
keywords: Angelica sinensis polysaccharide (ASP); Astragalus membranaceus polysaccharide (AMP); lipid peroxidation; oxidative stress; peroxydation lipidique; polysaccharide d’Angelica sinensis (PAS); polysaccharide d’Astragalus membranaceus (PAM); stress oxydant; superoxide dismutase (SOD); superoxyde dismutase (SOD)