title: Heterogeneous red blood cell adhesion and deformability in sickle cell disease.
abstract: We present a microfluidic approach that allows simultaneous interrogation of RBC properties in physiological flow conditions at a single cell level. With this method, we studied healthy hemoglobin A (HbA) and homozygous sickle hemoglobin (HbS) containing RBCs using whole blood samples from twelve subjects. We report that HbS-containing RBCs are heterogeneous in terms of adhesion and deformability in flow.
authors.affiliation: Case Biomanufacturing and Microfabrication Laboratory, Mechanical and Aerospace Engineering Department, Case Western Reserve University, Cleveland, OH, 44106, USA.
authors.forename: Yunus
authors.initials: Y
authors.lastname: Alapan
authors.affiliation: 1] Department of Hematology and Oncology, School of Medicine, Case Western Reserve University, Cleveland, OH, 44106, USA [2] Seidman Cancer Center at University Hospitals, Case Medical Center, Cleveland, OH, 44106, USA.
authors.forename: Jane A
authors.initials: JA
authors.lastname: Little
authors.affiliation: 1] Case Biomanufacturing and Microfabrication Laboratory, Mechanical and Aerospace Engineering Department, Case Western Reserve University, Cleveland, OH, 44106, USA [2] Department of Orthopaedics, Case Western Reserve University, Cleveland, OH, 44106, USA [3] Advanced Platform Technology Center, Louis Stokes Cleveland Veterans Affairs Medical Center, Cleveland, OH, 44106, USA.
authors.forename: Umut A
authors.initials: UA
authors.lastname: Gurkan
mesh_terms: D000755:Anemia, Sickle Cell / Q000473:pathology; D001783:Blood Flow Velocity; D002448:Cell Adhesion; D004907:Erythrocyte Deformability / Q000502:physiology*; D004912:Erythrocytes / Q000166:cytology / Q000502:physiology*; D006441:Hemoglobin A / Q000737:chemistry; D006451:Hemoglobin, Sickle / Q000737:chemistry; D006801:Humans; D046210:Microfluidic Analytical Techniques; D033081:Shear Strength
chemical_list: D006451:Hemoglobin, Sickle; D006441:Hemoglobin A