title: Antibiotic oxylipins from Alternanthera brasiliana and its endophytic bacteria.
abstract: Bioassay-guided fractionation of Alternanthera brasiliana stem extracts resulted in the isolation of an antibiotically active fraction. Five human pathogenic bacteria were used to guide the fractionation process for the isolation of antimicrobial compounds. Finally, 17 linoleate oxylipins were identified by LC-MS/MS and NMR spectroscopy. Five of the isolated compounds present in A. brasiliana tissues were also detected to be synthesized by endophytic bacteria of the genus Bacillus that were isolated from A. brasiliana. It is speculated that the antibiotic oxylipins from A. brasiliana might derive from bacteria and be involved in an ecological relationship between this plant and its endophytes.
authors.affiliation: LaBioMMi, Federal University of São Carlos, Rodovia Washington Luiz, km 235, CP 676, São Carlos - SP 13.565-905, Brazil; Department of Bioorganic Chemistry, Max Planck Institute for Chemical Ecology, Hans-Knöll-Straße 8, Jena, Germany. Electronic address: mariliatrapp@gmail.com.
authors.forename: Marília Almeida
authors.initials: MA
authors.lastname: Trapp
authors.affiliation: Research Group of Mass Spectrometry/Proteomics, Max Planck Institute for Chemical Ecology, Hans-Knöll-Straße 8, Jena, Germany.
authors.forename: Marco
authors.initials: M
authors.lastname: Kai
authors.affiliation: Department of Bioorganic Chemistry, Max Planck Institute for Chemical Ecology, Hans-Knöll-Straße 8, Jena, Germany.
authors.forename: Axel
authors.initials: A
authors.lastname: Mithöfer
authors.affiliation: LaBioMMi, Federal University of São Carlos, Rodovia Washington Luiz, km 235, CP 676, São Carlos - SP 13.565-905, Brazil.
authors.forename: Edson
authors.initials: E
authors.lastname: Rodrigues-Filho
mesh_terms: D027703:Amaranthaceae / Q000737:chemistry*; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D001412:Bacillus subtilis / Q000187:drug effects; D060026:Endophytes / Q000737:chemistry*; D004926:Escherichia coli / Q000187:drug effects; D006801:Humans; D008826:Microbial Sensitivity Tests; D016982:Micrococcus luteus / Q000187:drug effects; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D054883:Oxylipins / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D011550:Pseudomonas aeruginosa / Q000187:drug effects; D013211:Staphylococcus aureus / Q000187:drug effects
chemical_list: D000900:Anti-Bacterial Agents; D054883:Oxylipins
keywords: Alternanthera brasiliana; Amaranthaceae; Antibiotics; Bacillus; Endophytes; Fatty acids; LC–MS; Oxylipins