title: Uterine flushing with supernatant embryo culture medium in vitrified warmed blastocyst transfer cycles: a randomized controlled trial.
abstract: OBJECTIVE
  Does transfer of supernatant embryo culture fluid (stimulation of endometrial embryo transfer - SEET) prior to vitrified warmed blastocyst transfer result in better clinical pregnancy and live birth rates than direct vitrified warmed blastocyst transfer?
  
  
  METHODS
  This randomized controlled trial compared SEET group and direct transfer group (control) in 60 women undergoing vitrified warmed blastocyst transfers. The duration of the study was 3 years. The patients were undergoing vitrified warmed blastocyst transfer at university level infertility centre. Sixty women were randomized to SEET (n = 30) or control (n = 30).
  
  
  RESULTS
  Data was available for analysis from all the 30 women in the SEET group and 30 women in the control group. There were no drop outs in the trial. The implantation rate was significantly lower in the SEET group compared to the control group (27 vs. 44 %, P = 0.018). The clinical pregnancy rates were similar in both the groups (47 vs. 53 %) but the live birth rate was also significantly lower in SEET group (23 vs. 50 %, P = 0.03).
  
  
  CONCLUSIONS
  The sample size based on clinical pregnancy rates was small and hence not adequately powered to detect differences in live birth rates. Lack of blinding leading to possible bias cannot be ruled out.
  
  
  CONCLUSIONS
  There was no evidence of an improvement in clinical pregnancy rate following SEET in vitrified warmed blastocyst transfer compared to direct transfer.
authors.affiliation: Reproductive Medicine Unit, Christian Medical College Hospital, Vellore, Tamil Nadu, 632004, India.
authors.forename: Mohan S
authors.initials: MS
authors.lastname: Kamath
authors.forename: Mariona
authors.initials: M
authors.lastname: Mascarenhas
authors.forename: Kanchanadevi
authors.initials: K
authors.lastname: B
authors.forename: Nidhi N
authors.initials: NN
authors.lastname: Vasani
authors.forename: Asmita
authors.initials: A
authors.lastname: Joshi
authors.forename: Muthukumar
authors.initials: M
authors.lastname: K
authors.forename: Korula
authors.initials: K
authors.lastname: George
mesh_terms: D000328:Adult; D001723:Birth Rate; D015925:Cryopreservation; D003470:Culture Media; D046149:Embryo Culture Techniques / Q000379:methods*; D010064:Embryo Implantation / Q000502:physiology; D004624:Embryo Transfer / Q000379:methods*; D005260:Female; D006801:Humans; D011247:Pregnancy; D011256:Pregnancy Outcome; D018873:Pregnancy Rate; D014599:Uterus / Q000502:physiology; D058989:Vitrification
chemical_list: D003470:Culture Media