title: Therapeutic effect of topical ozonated oil on the epithelial healing of palatal wound sites: a planimetrical and cytological study.
abstract: OBJECTIVE
  To evaluate the effect of ozonated oil on palatal wounds.
  
  
  METHODS
  Eighteen patients were randomized and allocated to either the ozone group (n = 8) or control (n = 10) group. Free gingival graft surgery was performed, and post-harvested palatal wounds were treated with either 2 mL ozonated oil or control oil daily for 1 week. A planimetrical analysis analyzed the digital image for the wound sizes and shape factor at baseline, at 24 h, and days 5, 7, 14, 21, and 28, postoperatively. A cytological analysis used the keratinization and superficial cell indices at baseline, 24 h, and days 3, 7, 14, and 21 and the second and third months, postoperatively.
  
  
  RESULTS
  Planimetrical results showed a significant (P ≤ 0.05) improvement in wound size on days 5, 7, 14, 21, and 28, postoperatively, in the ozone group compared to the control group. Cytological results showed a significant (P ≤ 0.001) improvement in epithelial healing on days 7, 14, and 21, and the second and third months, postoperatively, after the application of ozonated oil compared to control oil.
  
  
  CONCLUSIONS
  Our results showed significant improvement in wound size and epithelial healing after topical ozonated oil application compared to control oil on palatal wounds.
authors.affiliation: Department of Periodontology, Jagadguru Sri Shivarathreeshwara Dental College and Hospital (JSS University), Mysore, Karnataka, India Department of Oral Pathology and Microbiology, Jagadguru Sri Shivarathreeshwara Dental College and Hospital (JSS University), Mysore, Karnataka, India Kalindi Oro Care, Varanasi, Uttar Pradesh, India.
authors.forename: Punit Vaibhav
authors.initials: PV
authors.lastname: Patel
authors.forename: Veerendra
authors.initials: V
authors.lastname: Kumar
authors.forename: Sheela
authors.initials: S
authors.lastname: Kumar
authors.forename: Vidya
authors.initials: V
authors.lastname: Gd
authors.forename: Amrita
authors.initials: A
authors.lastname: Patel
mesh_terms: D000287:Administration, Topical; D000328:Adult; D003581:Cytodiagnosis / Q000379:methods; D003774:Dental Plaque Index; D005260:Female; D005500:Follow-Up Studies; D005881:Gingiva / Q000187:drug effects / Q000637:transplantation*; D005889:Gingival Recession / Q000601:surgery; D005890:Gingivectomy / Q000379:methods; D006801:Humans; D007633:Keratins / Q000032:analysis; D008137:Longitudinal Studies; D008297:Male; D000069463:Olive Oil; D010126:Ozone / Q000008:administration & dosage / Q000627:therapeutic use*; D010919:Placebos; D010938:Plant Oils / Q000008:administration & dosage / Q000627:therapeutic use*; D062326:Re-Epithelialization / Q000187:drug effects; D012038:Regeneration / Q000187:drug effects; D060053:Transplant Donor Site / Q000473:pathology; D014945:Wound Healing / Q000187:drug effects; D055815:Young Adult
chemical_list: D000069463:Olive Oil; D010919:Placebos; D010938:Plant Oils; D010126:Ozone; D007633:Keratins
keywords: cytological technique; gingival recession; ozonide; postoperative; wound healing