title: Melon (Cucumis melo).
abstract: Genetic transformation is an important technique used in plant breeding and to functionally characterize genes of interest. The earliest reports of Agrobacterium-mediated transformation in the melon (Cucumis melo) were from the early 1990s (Fang and Grumet, Plant Cell Rep, 9: 160-164, 1990; Dong et al., Nat Biotechnol 9: 858-863, 1991; Valles and Lasa, Plant Cell Rep 13: 145-148, 1994). These early studies described three problems that decreased the efficiency of transformation: tetraploidy, chimeras, and escape. Using a liquid culture system for somatic embryogenesis, Akasaka-Kenedy et al. (Plant Sci 166: 763-769, 2004) overcame these problems and established an efficient transformation system; the protocol introduced in this chapter is based on this method.
authors.affiliation: University of Tsukuba, Ten-nodai 1-1-1, Tsukuba, Ibaraki, 305-8572, Japan.
authors.forename: Satoko
authors.initials: S
authors.lastname: Nonaka
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Ezura
mesh_terms: D000064:Acclimatization; D016960:Agrobacterium tumefaciens / Q000235:genetics / Q000254:growth & development; D018920:Coculture Techniques; D028463:Cucumis melo / Q000235:genetics* / Q000254:growth & development* / Q000502:physiology; D004780:Environment, Controlled; D005818:Genetic Engineering / Q000379:methods*; D030821:Plants, Genetically Modified; D012038:Regeneration; D012639:Seeds / Q000254:growth & development; D014170:Transformation, Genetic