title: [Effects of three different adult stem cells on inflammatory status of lipopolysaccharide- induced RAW264.7 cells].
abstract: OBJECTIVE
  To compare the modulatory effects of human amniotic epithelial cells (H-AECs), human amniotic mesenchymal cell (HA-MSCs), umbilical mesenchymal cells (UC-MSCs) on the inflammatory status of lipopolysaccharide (LPS)-induced RAW264.7 cells.
  
  
  METHODS
  RAW264.7 cells stimulated with LPS were co-cultured with H-AECs, HA-MSCs, or UC-MSCs or cultured in conditioned media of the 3 stem cells to assess the changes of the inflammatory status of RAW264.7 cells. The migration ability, nitric oxide concentration, and expressions of the pro-inflammatory and anti-inflammatory genes, including interleukin-1β (IL-1β), tumor necrosis factor-α (TNFα), and inducible nitric oxide synthase (NOS-2) of M1 macrophages, and Arg-1, CD206, and CD36 of M2 macrophages, were detected in the co-cultures.
  
  
  RESULTS
  Compared with the control macrophages, RAW264.7 cells cultured in the conditioned media of H-AECs, HA-MSCs, and UC-MSCs all showed significantly lowered migration abilities (P<0.05). Co-culture with H-AECs, but not the other two stem cells, resulted in a significant reduction of NO production (P<0.05) and significant down-regulation of IL-1β, TNFα, NOS-2, and INFβ expressions in RAW264.7 cells; co-culture with HA-MSCs and UC-MSCs only caused a down-regulation of INFβ mRNA expression. In all the 3 RAW264.7 and stem cell co-cultures, the expressions of the inflammation related genes including Arg-1, CD206, and CD36 were up-regulated significantly.
  
  
  CONCLUSIONS
  H-AECs, HA-MSCs, and UC-MSCs can all prevent RAW264.7 cells from differentiating into M2 macrophages, but their effects and mechanisms are different from one another.
authors.affiliation: Central South University Reproductive And Stem Cell Research Institute, Changsha 410000, China. E-mail: nancydada@126.com.
authors.forename: Da
authors.initials: D
authors.lastname: He
authors.forename: Lin
authors.initials: L
authors.lastname: Peng
authors.forename: Shengjian
authors.initials: S
authors.lastname: Huang
authors.forename: Wenling
authors.initials: W
authors.lastname: Lu
authors.forename: Jian
authors.initials: J
authors.lastname: Wang
mesh_terms: D053687:Adult Stem Cells / Q000166:cytology*; D000818:Animals; D002460:Cell Line; D018920:Coculture Techniques; D017077:Culture Media, Conditioned; D015536:Down-Regulation; D006801:Humans; D007249:Inflammation; D053583:Interleukin-1beta / Q000378:metabolism; D008070:Lipopolysaccharides; D008264:Macrophages / Q000166:cytology*; D059630:Mesenchymal Stem Cells / Q000166:cytology; D051379:Mice; D009569:Nitric Oxide / Q000378:metabolism; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D017077:Culture Media, Conditioned; D053583:Interleukin-1beta; D008070:Lipopolysaccharides; D014409:Tumor Necrosis Factor-alpha; D009569:Nitric Oxide; D052247:Nitric Oxide Synthase Type II; C496317:Nos2 protein, mouse