title: SAD1, an RNA polymerase I subunit A34.5 of rice, interacts with Mediator and controls various aspects of plant development.
abstract: The DWARF14 (D14) gene of rice functions within the signaling pathway of strigolactones, a group of plant hormones that inhibits shoot branching. We isolated a recessive mutant named super apical dormant (sad1-1) from a suppressor screen of d14-1. The growth of tillers (vegetative shoot branches) is suppressed in both the d14-1 sad1-1 double mutant and the sad1-1 single mutant. In addition, the sad1-1 mutant shows pleiotropic defects throughout development. SAD1 encodes an ortholog of RPA34.5, a subunit of RNA polymerase I (Pol I). Consequently, the level of ribosomal RNA (rRNA) is severely reduced in the sad1-1 mutant. These results indicate that proper ribosome function is a prerequisite for normal development in plants. The Arabidopsis ortholog of SAD1 was previously isolated as a Mediator-interacting protein. Here we show that SAD1 interacts physically with the Mediator complex through direct binding with OsMED4, a component of the middle module of the Mediator complex in rice. It is known that Mediator interacts with Pol II, which transcribes mRNAs and functions as a central regulator of transcription. This study indicates a novel aspect of Mediator function in Pol I-controlled rRNA transcription. TFIIF2 and RPC53 are the counterparts of RPA34.5 in Pol II and Pol III, respectively. We demonstrate that the rice orthologs of these proteins also interact with OsMED4. Our results suggest that interaction with MED4 in the Mediator complex is a common feature of the three types of RNA polymerases.
authors.affiliation: Graduate School of Agriculture and Life Sciences, University of Tokyo, Yayoi, Bunkyo, Tokyo, 113-8657, Japan.
authors.forename: Weiqiang
authors.initials: W
authors.lastname: Li
authors.forename: Akiko
authors.initials: A
authors.lastname: Yoshida
authors.forename: Megumu
authors.initials: M
authors.lastname: Takahashi
authors.forename: Masahiko
authors.initials: M
authors.lastname: Maekawa
authors.forename: Mikiko
authors.initials: M
authors.lastname: Kojima
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Sakakibara
authors.forename: Junko
authors.initials: J
authors.lastname: Kyozuka
mesh_terms: D018506:Gene Expression Regulation, Plant; D012275:Oryza / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D018520:Plant Shoots / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism*; D012318:RNA Polymerase I / Q000235:genetics / Q000378:metabolism*
chemical_list: D010940:Plant Proteins; D012318:RNA Polymerase I
keywords: D14; Mediator; Pol I; rice; shoot branching