title: Ontogenetic changes in the olfactory antennules of the shore crab, Hemigrapsus oregonensis, maintain sniffing function during growth.
abstract: Malacostracan crustaceans capture odours using arrays of chemosensory hairs (aesthetascs) on antennules. Lobsters and stomatopods have sparse aesthetascs on long antennules that flick with a rapid downstroke when water flows between the aesthetascs and a slow return stroke when water is trapped within the array (sniffing). Changes in velocity only cause big differences in flow through an array in a critical range of hair size, spacing and speed. Crabs have short antennules bearing dense arrays of flexible aesthetascs that splay apart during downstroke and clump together during return. Can crabs sniff, and when during ontogeny are they big enough to sniff? Antennules of Hemigrapsus oregonensis representing an ontogenetic series from small juveniles to adults were used to design dynamically scaled physical models. Particle image velocimetry quantified fluid flow through each array and showed that even very small crabs capture a new water sample in their arrays during the downstroke and retain that sample during return stroke. Comparison with isometrically scaled antennules suggests that reduction in aesthetasc flexural stiffness during ontogeny, in addition to increase in aesthetasc number and decrease in relative size, maintain sniffing as crabs grow. Sniffing performance of intermediate-sized juveniles was worse than for smaller and larger crabs.
authors.affiliation: Department of Mathematics, University of North Carolina, CB# 3250, Chapel Hill, NC 27599-3250, USA Department of Integrative Biology, University of California, Berkeley, CA, USA lwaldrop@email.unc.edu.
authors.forename: Lindsay D
authors.initials: LD
authors.lastname: Waldrop
authors.affiliation: Department of Integrative Biology, University of California, Berkeley, CA, USA.
authors.forename: Miranda
authors.initials: M
authors.lastname: Hann
authors.affiliation: Department of Integrative Biology, University of California, Berkeley, CA, USA Committee on Evolutionary Biology, University of Chicago, Chicago, IL, USA.
authors.forename: Amy K
authors.initials: AK
authors.lastname: Henry
authors.affiliation: Department of Integrative Biology, University of California, Berkeley, CA, USA.
authors.forename: Agnes
authors.initials: A
authors.lastname: Kim
authors.affiliation: Department of Integrative Biology, University of California, Berkeley, CA, USA.
authors.forename: Ayesha
authors.initials: A
authors.lastname: Punjabi
authors.affiliation: Department of Integrative Biology, University of California, Berkeley, CA, USA.
authors.forename: M A R
authors.initials: MA
authors.lastname: Koehl
mesh_terms: D000818:Animals; D001696:Biomechanical Phenomena; D001703:Biophysics; D049628:Body Size; D003386:Brachyura / Q000502:physiology*; D009812:Odorants; D012212:Rheology; D012679:Sense Organs / Q000737:chemistry*; D012903:Smell / Q000502:physiology*; D014872:Water Movements
keywords: aesthetasc; crab; fluid dynamics; olfaction; ontogeny; scaling