title: Saccharomyces cerevisiae SHSY detoxifies petroleum n-alkanes by an induced CYP52A58 and an enhanced order in cell surface hydrophobicity.
abstract: Environmental hydrocarbon contamination has a serious hazard to human health. Alkanes, the major component of hydrocarbons, can be consumed by various species of yeast. We previously identified a new strain SHSY of Saccharomyces cerevisiae with a remarkable ability to utilize the petroleum crude-oil (PCO) in aqueous solution. The current study demonstrated that the n-alkanes-assimilation activity of S. cerevisiae SHSY was related to an induced microsomal protein of 59 kDa approximately. The identified ORF encoded a protein of 517 amino acids and shared 93% sequence identity with an alkane-inducible hydroxylase CYP52A53 isolated from Scheffersomyces stipitis CBS. It was therefore referred as CYP52A58. The catalytic activity of the recombinant CYP52A58 was confirmed by the hydroxylation of n-alkanes, it showed an optimal mono-terminal hydroxylation activity toward n-hexadecane. Moreover, the ability of the yeast to use n-alkanes was accompanied with an increasing level in cell wall mannoproteins. Two differential protein bands were detected in the mannoproteins extracted from PCO-grown yeast. In parallel, a significant increase in the fatty acids content with a high degree of unsaturation was subsequently detected in the PCO-grown yeast. This study characterizes a safe and potential microorganism to remove n-alkanes from the aquatic environment.
authors.affiliation: Department of Molecular Biology and Biotechnology, Atomic Energy Commission of Syria (AECS), B.P. Box 6091, Damascus, Syria. Electronic address: ashanano@aec.org.sy.
authors.forename: Abdulsamie
authors.initials: A
authors.lastname: Hanano
authors.affiliation: Department of Molecular Biology and Biotechnology, Atomic Energy Commission of Syria (AECS), B.P. Box 6091, Damascus, Syria.
authors.forename: Mouhnad
authors.initials: M
authors.lastname: Shaban
authors.affiliation: Department of Molecular Biology and Biotechnology, Atomic Energy Commission of Syria (AECS), B.P. Box 6091, Damascus, Syria.
authors.forename: Ibrahem
authors.initials: I
authors.lastname: Almousally
authors.affiliation: Department of Chemistry, Atomic Energy Commission of Syria (AECS), B.P. Box 6091, Damascus, Syria.
authors.forename: Mahmoud
authors.initials: M
authors.lastname: Al-Ktaifani
mesh_terms: D000473:Alkanes; D001673:Biodegradation, Environmental; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D004785:Environmental Pollutants / Q000378:metabolism*; D005227:Fatty Acids / Q000378:metabolism; D006838:Hydrocarbons; D057927:Hydrophobic and Hydrophilic Interactions; D006900:Hydroxylation; D008658:Inactivation, Metabolic; D010578:Petroleum / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000378:metabolism*
chemical_list: D000473:Alkanes; D004785:Environmental Pollutants; D005227:Fatty Acids; D006838:Hydrocarbons; D010578:Petroleum; D003577:Cytochrome P-450 Enzyme System; C007932:n-hexadecane
keywords: CYP52A58; Detoxification; Saccharomyces cerevisiae SHSY; n-Alkanes