title: The role of miR-34a in the hepatoprotective effect of hydrogen sulfide on ischemia/reperfusion injury in young and old rats.
abstract: Hydrogen sulfide (H2S) can protect the liver against ischemia-reperfusion (I/R) injury. However, it is unknown whether H2S plays a role in the protection of hepatic I/R injury in both young and old patients. This study compared the protective effects of H2S in a rat model (young and old animals) of I/R injury and the mechanism underlying its effects. Young and old rats were assessed following an injection of NaHS. NaHS alone reduced hepatic I/R injury in the young rats by activating the nuclear erythroid-related factor 2 (Nrf2) signaling pathway, but it had little effect on the old rats. NaHS pretreatment decreased miR-34a expression in the hepatocytes of the young rats with hepatic I/R. Overexpression of miR-34a decreased Nrf-2 and its downstream target expression, impairing the hepatoprotective effect of H2S on the young rats. More importantly, downregulation of miR-34a expression increased Nrf-2 and the expression of its downstream targets, enhancing the effect of H2S on hepatic I/R injury in the old rats. This study reveals the different effects of H2S on hepatic I/R injury in young and old rats and sheds light on the involvement of H2S in miR-34a modulation of the Nrf-2 pathway.
authors.affiliation: Center of Liver Transplantation, The First Affiliated Hospital of Nanjing Medical University, The Key Laboratory of Living Donor Liver Transplantation, Ministry of Health, Nanjing, China.
authors.forename: Xinli
authors.initials: X
authors.lastname: Huang
authors.affiliation: Center of Liver Transplantation, The First Affiliated Hospital of Nanjing Medical University, The Key Laboratory of Living Donor Liver Transplantation, Ministry of Health, Nanjing, China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Gao
authors.affiliation: Center of Liver Transplantation, The First Affiliated Hospital of Nanjing Medical University, The Key Laboratory of Living Donor Liver Transplantation, Ministry of Health, Nanjing, China.
authors.forename: Jianjie
authors.initials: J
authors.lastname: Qin
authors.affiliation: Center of Liver Transplantation, The First Affiliated Hospital of Nanjing Medical University, The Key Laboratory of Living Donor Liver Transplantation, Ministry of Health, Nanjing, China.
authors.forename: Sen
authors.initials: S
authors.lastname: Lu
mesh_terms: D000367:Age Factors; D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D015153:Blotting, Western; D002478:Cells, Cultured; D015870:Gene Expression / Q000187:drug effects; D022781:Hepatocytes / Q000187:drug effects / Q000378:metabolism; D006862:Hydrogen Sulfide / Q000097:blood / Q000494:pharmacology*; D008099:Liver / Q000098:blood supply / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D051267:NF-E2-Related Factor 2 / Q000235:genetics / Q000378:metabolism; D020011:Protective Agents / Q000494:pharmacology; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000097:blood / Q000235:genetics / Q000517:prevention & control*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction / Q000187:drug effects / Q000235:genetics; D013440:Sulfides / Q000097:blood / Q000494:pharmacology
chemical_list: C560944:MIRN34 microRNA, rat; D035683:MicroRNAs; D051267:NF-E2-Related Factor 2; D020011:Protective Agents; D013440:Sulfides; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; C025451:sodium bisulfide; D006862:Hydrogen Sulfide