title: Exploration of genetic diversity among medicinally important genus Epimedium species based on genomic and EST-SSR marker.
abstract: Epimedium species has gained prime importance due to their medicinal and economic values. Therefore, in this study, 26 genomic SSR and 10 EST-SSR markers were developed for 13 medicinal species of the Epimedium genus and one out-group species Vancouveria hexandra W. J. Hooker to explore the existing genetic diversity. A total of 100 alleles by genomic SSR and 65 by EST-SSR were detected. The genomic SSR markers were presented between 2-7 alleles per locus. The observed heterozygosity (Ho) and expected heterozygosity (He) ranged from 0.00 to 4.5 and 0.0254 to 2.8108, respectively. Similarly, for EST-SSR, these values were ranged from 3.00 to 4.00 and 1.9650 to 2.7142. The number of alleles for EST-SSR markers ranged from 3 to 10 with an average of 3.51 per loci. It has been concluded that medicinally important species of the genus Epimedium possesses lower intraspecific genetic variation.
authors.affiliation: a Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences , Gaungzhou 510650 , PR China.
authors.forename: Zubaida
authors.initials: Z
authors.lastname: Yousaf
authors.forename: Weiming
authors.initials: W
authors.lastname: Hu
authors.forename: Yanjun
authors.initials: Y
authors.lastname: Zhang
authors.forename: Shaohua
authors.initials: S
authors.lastname: Zeng
authors.forename: Ying
authors.initials: Y
authors.lastname: Wang
mesh_terms: D000483:Alleles; D034181:Epimedium / Q000235:genetics*; D020224:Expressed Sequence Tags; D005819:Genetic Markers; D014644:Genetic Variation; D023281:Genomics; D018895:Microsatellite Repeats; D010946:Plants, Medicinal / Q000235:genetics
chemical_list: D005819:Genetic Markers
keywords: Epimedium; Systematics; genetic diversity; genomic SSR/EST SSR markers