title: Polymorphism in mtDNA control region of Mizo-Mongloid Breast Cancer samples as revealed by PCR-RFLP analysis.
abstract: Mutations in mitochondrial D-loop region of DNA (mtDNA) may serve as a potential sensor for cellular DNA damage and marker for cancer development. We investigated the restriction fragment length polymorphism (RFLP) pattern of the D-loop region in the blood samples of breast cancer patients among Mizoram population. Significant differences were observed among breast cancer and healthy blood samples in the RFLP pattern using AluI, HaeIII and RsaI enzymes. Polymorphic information content (PIC - 0.258), band informativeness (∑Ib - 3.283) and marker index (MI - 0.006) were highest in the case of RsaI enzyme. Our data suggest that the RsaI polymorphic site in the mitochondrial control region is an informative marker for breast cancer development in Mizo population.
authors.affiliation: a Department of Biotechnology , Mizoram University , Aizawl , Mizoram , India and.
authors.forename: Souvik
authors.initials: S
authors.lastname: Ghatak
authors.affiliation: b Mizoram State Cancer Institute , Zemabawk, Aizawl , Mizoram , India.
authors.forename: Doris
authors.initials: D
authors.lastname: Lallawmzuali
authors.affiliation: a Department of Biotechnology , Mizoram University , Aizawl , Mizoram , India and.
authors.forename: Subhajit
authors.initials: S
authors.lastname: Mukherjee
authors.affiliation: b Mizoram State Cancer Institute , Zemabawk, Aizawl , Mizoram , India.
authors.forename: Lal
authors.initials: L
authors.lastname: Mawia
authors.affiliation: b Mizoram State Cancer Institute , Zemabawk, Aizawl , Mizoram , India.
authors.forename: Jeremy L
authors.initials: JL
authors.lastname: Pautu
authors.affiliation: a Department of Biotechnology , Mizoram University , Aizawl , Mizoram , India and.
authors.forename: Nachimuthu Senthil
authors.initials: NS
authors.lastname: Kumar
mesh_terms: D014408:Biomarkers, Tumor / Q000235:genetics; D001943:Breast Neoplasms / Q000097:blood / Q000235:genetics*; D004272:DNA, Mitochondrial / Q000235:genetics; D005260:Female; D050259:Genes, Mitochondrial; D016678:Genome / Q000235:genetics; D054629:Genome, Mitochondrial / Q000235:genetics*; D006801:Humans; D008928:Mitochondria / Q000235:genetics; D008986:Mongolia; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length / Q000235:genetics; D017422:Sequence Analysis, DNA / Q000379:methods; D000073336:Whole Genome Sequencing / Q000379:methods
chemical_list: D014408:Biomarkers, Tumor; D004272:DNA, Mitochondrial
keywords: Haplogroups; PCR-RFLP; marker index; mitochondrial DNA; polymorphism