title: Methylation status and immunohistochemistry of BRCA1 in epithelial ovarian cancer.
abstract: BACKGROUND
  Cancer initiation and progression are controlled by genetic and epigenetic events. One epigenetic process which is widely known is DNA methylation, a cause of gene silencing. If a gene is silenced the protein which it encodes will not expressed.
  
  
  OBJECTIVE
  1. Identify the methylation status of BRCA1 in patients with epithelial ovarian cancer (EOC)and assess BRCA1 protein expression in tumor tissue. 2. Examine whether BRCA1 gene methylation and BRCA1 protein are associated with survival of epithelial ovarian cancer patients.
  
  
  METHODS
  The study design was a prospective-cohort study, conducted at Sardjito hospital, Yogyakarta, Indonesia.
  
  
  RESULTS
  A total of 69 cases were analyzed in this study. The data showed that the methylation status of BRCA1 in EOC was positive in 89.9%, with clear protein expression of BRCA1 in 31.9%. Methylation status and expression of BRCA1 were not prognosticators of EOC patients. Menarche, CA125 level, clinical stage and residual tumor were independent factors for prognosis.
authors.affiliation: Department of Obstetric and Gynecology, Faculty of Medicine, Universitas Gadjah Mada, Yogyakarta, Indonesia E-mail : pradjatmo@yahoo.com; herupradjatmo@gmail.com.
authors.forename: Heru
authors.initials: H
authors.lastname: Pradjatmo
authors.forename: Djaswadi
authors.initials: D
authors.lastname: Dasuki
authors.forename: Mohammad
authors.initials: M
authors.lastname: Anwar
authors.forename: Sofia
authors.initials: S
authors.lastname: Mubarika
authors.lastname: Harijadi
mesh_terms: D000328:Adult; D019313:BRCA1 Protein / Q000235:genetics*; D000077216:Carcinoma, Ovarian Epithelial; D019175:DNA Methylation; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D009375:Neoplasms, Glandular and Epithelial / Q000235:genetics* / Q000473:pathology*; D010051:Ovarian Neoplasms / Q000235:genetics* / Q000473:pathology*; D011379:Prognosis; D011446:Prospective Studies
chemical_list: D019313:BRCA1 Protein