title: Comparison of pharmacokinetics of marbofloxacin after subcutaneous administration of various multiple-dose regimens to water buffalo calves (Bubalus bubalis).
abstract: OBJECTIVE
  To determine pharmacokinetics of marbofloxacin in water buffalo calves (Bubalus bubalis) after multiple SC administrations and to assess differences in regimen efficacy.
  
  
  METHODS
  18 healthy buffalo calves.
  
  
  METHODS
  Calves (n = 6 calves/group) were assigned to receive marbofloxacin SC in the neck at 1 of 3 dosages (2 mg/kg, q 24 h for 6 days [regimen 1]; 4 mg/kg, q 48 h for 6 days [regimen 2]; and 4 mg/kg, q 24 h for 3 days [regimen 3]). Serum marbofloxacin concentrations were analyzed. Efficacy predictors were estimated on the basis of minimum inhibitory concentration and mutant prevention concentration reported for Pasteurella multocida and Mannheimia haemolytica.
  
  
  RESULTS
  Mean ± SD area under the concentration-time curve was 5.92 ± 0.40 μg•h/mL for regimen 1, which differed significantly from that for regimens 2 (14.26 ± 0.92 μg•h/mL) and 3 (14.17 ± 0.51 μg•h/mL). Mean residence time and mean elimination half-life for regimen 2 (9.93 ± 0.20 hours and 8.77 ± 0.71 hours) both differed significantly from those for regimens 1 (721 ± 0.11 hours and 5.71 ± 0.38 hours) and 3 (759 ± 0.13 hours and 737 ± 1.19 hours). Values obtained from indices for P multocida and M haemolytica had an excessively wide range because of the various degrees of antimicrobial susceptibility (low, medium, and high) of the strains.
  
  
  CONCLUSIONS
  Regimen 3 had the most favorable indices, and it would be conducive for owner compliance and require less handling of animals.
authors.affiliation: Department of Pharmacology, Faculty of Veterinary Science, Universidad Nacional del Litoral, 2805, Esperanza 3000, Santa Fe, Argentina.
authors.forename: Eduardo E
authors.initials: EE
authors.lastname: Baroni
authors.forename: Sonia
authors.initials: S
authors.lastname: Rubio
authors.forename: José J
authors.initials: JJ
authors.lastname: De Lucas
authors.forename: María D San
authors.initials: MD
authors.lastname: Andrés
authors.forename: Manuel I San
authors.initials: MI
authors.lastname: Andrés
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000097:blood / Q000493:pharmacokinetics*; D019540:Area Under Curve; D002020:Buffaloes / Q000097:blood*; D024841:Fluoroquinolones / Q000008:administration & dosage / Q000097:blood / Q000493:pharmacokinetics*; D006207:Half-Life; D008826:Microbial Sensitivity Tests
chemical_list: D000900:Anti-Bacterial Agents; D024841:Fluoroquinolones; C080260:marbofloxacin