title: High impact of uranyl ions on carrying-releasing oxygen capability of hemoglobin-based blood substitutes.
abstract: The effect of radioactive UO2 (2+) on the oxygen-transporting capability of hemoglobin-based oxygen carriers has been investigated in vitro. The hemoglobin (Hb) microspheres fabricated by the porous template covalent layer-by-layer (LbL) assembly were utilized as artificial oxygen carriers and blood substitutes. Magnetic nanoparticles of iron oxide (Fe3 O4 ) were loaded in porous CaCO3 particles for magnetically assisted chemical separation (MACS). Through the adsorption spectrum of magnetic Hb microspheres after adsorbing UO2 (2+) , it was found that UO2 (2+) was highly loaded in the magnetic Hb microspheres, and it shows that the presence of UO2 (2+) in vivo destroys the structure and oxygen-transporting capability of Hb microspheres. In view of the high adsorption capacity of UO2 (2+) , the as-assembled magnetic Hb microspheres can be considered as a novel, highly effective adsorbent for removing metal toxins from radiation-contaminated bodies, or from nuclear-power reactor effluent before discharge into the environment.
authors.affiliation: Northwest Institute of Nuclear Technology, Xi'an, Shaanxi 710024 (P. R. China).
authors.forename: Li
authors.initials: L
authors.lastname: Duan
authors.forename: Lili
authors.initials: L
authors.lastname: Du
authors.forename: Yi
authors.initials: Y
authors.lastname: Jia
authors.forename: Wenyuan
authors.initials: W
authors.lastname: Liu
authors.forename: Zhichao
authors.initials: Z
authors.lastname: Liu
authors.forename: Junbai
authors.initials: J
authors.lastname: Li
mesh_terms: D000327:Adsorption; D001802:Blood Substitutes / Q000737:chemistry / Q000378:metabolism*; D002119:Calcium Carbonate / Q000737:chemistry; D002412:Cations / Q000737:chemistry / Q000378:metabolism; D005290:Ferric Compounds / Q000737:chemistry; D058185:Magnetite Nanoparticles / Q000737:chemistry; D010100:Oxygen / Q000378:metabolism*; D016062:Porosity; D014501:Uranium / Q000737:chemistry / Q000378:metabolism*
chemical_list: D001802:Blood Substitutes; D002412:Cations; D005290:Ferric Compounds; D058185:Magnetite Nanoparticles; C000499:ferric oxide; D014501:Uranium; D002119:Calcium Carbonate; D010100:Oxygen
keywords: LbL assembly; hemoglobin microspheres; magnetic Fe3O4 particles; porous CaCO3; uranyl ion