title: In vitro susceptibility of Brazilian Pythium insidiosum isolates to essential oils of some Lamiaceae family species.
abstract: The present study aimed to evaluate the in vitro antimicrobial action of Origanum vulgare, Origanum majorana, Mentha piperita and Rosmarinus officinalis on Pythium insidiosum oomycete zoospores. The antimicrobial activity evaluation was performed by the broth microdilution method according to CSLI M38-A2 documentation adapted to phytopharmaceuticals. Twenty-two P. insidiosum isolates were evaluated, and the minimum inhibitory concentration was determined at 100% growth inhibition. All P. insidiosum isolates evaluated showed a minimum inhibitory concentration ranging from 0.05 to 1.75 mg/mL when O. vulgare oil was used and from 0.11 to 3.5 mg/mL for O. majorana, M. piperita and R. officinalis oils. The results obtained indicate that the essential oils tested showed antimicrobial activity on P. insidiosum, with O. vulgare essential oil showing the best performance. These findings emphasize the potential use of plant essential oils as control agents in P. insidiosum infections; further research, however, is needed so as the in vivo activity of these oils can also be evaluated.
authors.affiliation: Departamento de Doenças Infecciosas, Universidade Federal de Pelotas (UFPel), Campus Universitário Capão do Leão, s/no, Pelotas, RS, 96010-900, Brazil.
authors.forename: A O S
authors.initials: AO
authors.lastname: Fonseca
authors.forename: D I B
authors.initials: DI
authors.lastname: Pereira
authors.forename: R G
authors.initials: RG
authors.lastname: Jacob
authors.forename: F S
authors.initials: FS
authors.lastname: Maia Filho
authors.forename: D H
authors.initials: DH
authors.lastname: Oliveira
authors.forename: B P
authors.initials: BP
authors.lastname: Maroneze
authors.forename: J S S
authors.initials: JS
authors.lastname: Valente
authors.forename: L G
authors.initials: LG
authors.lastname: Osório
authors.forename: S A
authors.initials: SA
authors.lastname: Botton
authors.forename: M C A
authors.initials: MC
authors.lastname: Meireles
mesh_terms: D000890:Anti-Infective Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D001938:Brazil; D019686:Lamiaceae / Q000737:chemistry*; D008826:Microbial Sensitivity Tests; D009822:Oils, Volatile / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D010938:Plant Oils / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D011775:Pythium / Q000187:drug effects* / Q000254:growth & development
chemical_list: D000890:Anti-Infective Agents; D009822:Oils, Volatile; D010938:Plant Oils