title: Multi-electrode array recordings of human epileptic postoperative cortical tissue.
abstract: Epilepsy, affecting about 1% of the population, comprises a group of neurological disorders characterized by the periodic occurrence of seizures, which disrupt normal brain function. Despite treatment with currently available antiepileptic drugs targeting neuronal functions, one third of patients with epilepsy are pharmacoresistant. In this condition, surgical resection of the brain area generating seizures remains the only alternative treatment. Studying human epileptic tissues has contributed to understand new epileptogenic mechanisms during the last 10 years. Indeed, these tissues generate spontaneous interictal epileptic discharges as well as pharmacologically-induced ictal events which can be recorded with classical electrophysiology techniques. Remarkably, multi-electrode arrays (MEAs), which are microfabricated devices embedding an array of spatially arranged microelectrodes, provide the unique opportunity to simultaneously stimulate and record field potentials, as well as action potentials of multiple neurons from different areas of the tissue. Thus MEAs recordings offer an excellent approach to study the spatio-temporal patterns of spontaneous interictal and evoked seizure-like events and the mechanisms underlying seizure onset and propagation. Here we describe how to prepare human cortical slices from surgically resected tissue and to record with MEAs interictal and ictal-like events ex vivo.
authors.affiliation: Neuroglial Interactions in Cerebral Physiopathology, Center for Interdisciplinary Research in Biology, CNRS UMR 7241, INSERM U1050, Collège de France.
authors.forename: Elena
authors.initials: E
authors.lastname: Dossi
authors.affiliation: Infantile Epilepsies & Brain Plasticity, INSERM U1129, PRES, Paris Descartes University, Sorbonne Paris Cité, CEA; Neurosurgery Department, Necker Hospital, AP-HP, Paris Descartes University.
authors.forename: Thomas
authors.initials: T
authors.lastname: Blauwblomme
authors.affiliation: Infantile Epilepsies & Brain Plasticity, INSERM U1129, PRES, Paris Descartes University, Sorbonne Paris Cité, CEA; Rare Epilepsies Reference Center, Necker Hospital, AP-HP, Paris Descartes University.
authors.forename: Rima
authors.initials: R
authors.lastname: Nabbout
authors.affiliation: Infantile Epilepsies & Brain Plasticity, INSERM U1129, PRES, Paris Descartes University, Sorbonne Paris Cité, CEA; Neurophysiology Department, La Pitié-Salpêtrière Hospital, AP-HP, Sorbonne and Pierre and Marie Curie University.
authors.forename: Gilles
authors.initials: G
authors.lastname: Huberfeld
authors.affiliation: Neuroglial Interactions in Cerebral Physiopathology, Center for Interdisciplinary Research in Biology, CNRS UMR 7241, INSERM U1050, Collège de France; nathalie.rouach@college-de-france.fr.
authors.forename: Nathalie
authors.initials: N
authors.lastname: Rouach
mesh_terms: D002540:Cerebral Cortex / Q000503:physiopathology* / Q000601:surgery; D003943:Diagnostic Techniques, Neurological; D004566:Electrodes; D055724:Electrophysiological Phenomena; D004827:Epilepsy / Q000134:cerebrospinal fluid / Q000503:physiopathology* / Q000601:surgery; D006801:Humans; D011182:Postoperative Care