title: [Nitric oxide mediated TNF-α, IL-1β gene expression in liver induced by crush injury of rat's soft tissues].
abstract: OBJECTIVE
  To explore the effect of nitric oxide (NO) on the gene expression of hepatic TNF-α and IL-1β by crush injury of rat's soft tissues.
  
  
  METHODS
  Rats were randomly divided into sham group, crush group, crush+aminoguanidine (AG) group, and crush+L-arginine (L-Arg) group. Activities of ALT and AST as well as NO level in serum were measured. Gene expressions of TNF-α and IL-1β were detected with RT-PCR.
  
  
  RESULTS
  Obvious increase in TNF-α and IL-1β mRNA expression was detected in the crush group compared with the sham group (P<0.05). After pretreated L-Arg, expressions of TNF-α and IL-1β mRNA were markedly increased (P<0.05). After pretreated AG, those indices obviously decreased (P<0.05). Activities of ALT and AST enhanced and NO level increased in the crush group compared with the sham group (P<0.05). Pretreatment with L-Arg or AG led to substantial increased or reduced activities of ALT and AST as well as NO levels, respectively.
  
  
  CONCLUSIONS
  Endogenous NO mediated TNF-α, IL-1β mRNA up expression in liver induced by increased production of NO after crush injury of rat's soft tissues.
authors.forename: Guo-kai
authors.initials: GK
authors.lastname: Dong
authors.forename: Xiao-tong
authors.initials: XT
authors.lastname: Zhang
authors.forename: Li-qin
authors.initials: LQ
authors.lastname: Ma
authors.forename: Na
authors.initials: N
authors.lastname: Li
authors.forename: Chun-ling
authors.initials: CL
authors.lastname: Ma
authors.forename: Bin
authors.initials: B
authors.lastname: Cong
authors.forename: Zhen-yong
authors.initials: ZY
authors.lastname: Gu
mesh_terms: D000818:Animals; D015870:Gene Expression; D053583:Interleukin-1beta / Q000378:metabolism*; D008099:Liver; D009569:Nitric Oxide / Q000502:physiology*; D012333:RNA, Messenger; D051381:Rats; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*; D014947:Wounds and Injuries
chemical_list: D053583:Interleukin-1beta; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; D009569:Nitric Oxide