title: The durative use of suspension cells and callus for volatile oil by comparative with seeds and fruits in Capparis spinosa L.
abstract: Capparis spinosa is one of the most important eremophytes among the medicinal plants, and continued destruction of these plants poses a major threat to species survival. The development of methods to extract compounds, especially those of medicinal value, without harvesting the whole plant is an issue of considerable socioeconomic importance. On the basis of an established system for culture of suspension cells and callus in vitro, Gas Chromatograph-Mass Spectrometer (GC-MS) was used for the volatile oil composition analyzing in seed, fruit, suspension cells and callus. Fatty acids were the major component, and the highest content of alkanes was detected in seed, with <1.0% in suspension cells and callus. Esters, olefins and heterocyclic compounds were significantly higher in fruit than in the other materials. The content of acid esters in the suspension cells and callus was significantly higher than in seed and fruit. This indicated that the suspension cells and callus could be helpful for increasing the value of volatile oil and replacing seeds and fruit partially as a source of some compounds of the volatile oil and may also produce some new medical compounds. The above results give valuable information for sustainable use of C. spinosa and provide a foundation for use of the C. spinosa suspension cells and callus as an ongoing medical resource.
authors.affiliation: College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, 430074, P. R. China; Key Laboratory of Molecular Biology, Physics of Ministry of Education, Huazhong University of Science and Technology, Wuhan, 430074, P. R. China.
authors.forename: Yongtai
authors.initials: Y
authors.lastname: Yin
authors.affiliation: Hubei Collaborative Innovation Center for Green Transformation of Bio-Resources, Faculty of Life Science, Hubei University, Wuhan, 430062, P. R. China.
authors.forename: Yuchi
authors.initials: Y
authors.lastname: He
authors.affiliation: College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, 430074, P. R. China.
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.affiliation: College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, 430074, P. R. China.
authors.forename: Lu
authors.initials: L
authors.lastname: Gan
authors.affiliation: College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, 430074, P. R. China; Key Laboratory of Molecular Biology, Physics of Ministry of Education, Huazhong University of Science and Technology, Wuhan, 430074, P. R. China.
authors.forename: Chunhua
authors.initials: C
authors.lastname: Fu
authors.affiliation: College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, 430074, P. R. China; Key Laboratory of Molecular Biology, Physics of Ministry of Education, Huazhong University of Science and Technology, Wuhan, 430074, P. R. China.
authors.forename: Haibo
authors.initials: H
authors.lastname: Jia
authors.affiliation: College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, 430074, P. R. China; Key Laboratory of Molecular Biology, Physics of Ministry of Education, Huazhong University of Science and Technology, Wuhan, 430074, P. R. China.
authors.forename: Maoteng
authors.initials: M
authors.lastname: Li
mesh_terms: D031484:Capparis / Q000737:chemistry* / Q000196:embryology; D008401:Gas Chromatography-Mass Spectrometry; D012639:Seeds / Q000737:chemistry*