title: Preventive effect of gomisin J from Schisandra chinensis on angiotensin II-induced hypertension via an increased nitric oxide bioavailability.
abstract: Gomisin J (GJ) is a small molecular weight lignan found in Schisandra chinensis and has been demonstrated to have vasodilatory activity. In this study, the authors investigated the effect of GJ on blood pressure (BP) in angiotensin II (Ang II)-induced hypertensive mice. In addition, we determined the relative potencies of gomisin A (GA) and GJ with respect to vasodilatory activity and antihypertensive effects. C57/BL6 mice infused s.c. with Ang II (2 μg kg(-1) min(-1) for 2 weeks) showed an increase in BP and a decrease in plasma nitric oxide (NO) metabolites. In the thoracic aortas of Ang II-induced hypertensive mice, a decrease in vascular NO was accompanied by an increase in reactive oxygen species (ROS) production. Furthermore, these alterations in BP, plasma concentrations of NO metabolites and in the vascular productions of NO and ROS in Ang II-treated mice were reversed by the co-administration of GJ (1 and 3 μg kg(-1) min(-1)). In in vitro studies, Ang II decreased the cellular concentration of NO, which was accompanied by a reduction in phosphorylated endothelial nitric oxide synthase (eNOS) and an increase in ROS production. These eNOS phosphorylation and ROS production changes in Ang II-treated cells were also reversed by GJ pretreatment (0-3 μg ml(-1)). Interestingly, the vasodilatory and antihypertensive effects of GJ were more prominent than those of GA. Collectively, an increase in BP in mice treated with Ang II was markedly attenuated by GJ, which was attributed to the preservations of vascular NO bioavailability and eNOS function, and to the inhibition of ROS production in Ang II-induced hypertensive mice.
authors.affiliation: Department of Pharmacology, School of Medicine and MRC for Ischemic Tissue Regeneration, Pusan National University, Yangsan, Gyeongnam, Republic of Korea.
authors.forename: Byeong Hyeok
authors.initials: BH
authors.lastname: Ye
authors.affiliation: Department of Pharmacology, School of Medicine and MRC for Ischemic Tissue Regeneration, Pusan National University, Yangsan, Gyeongnam, Republic of Korea.
authors.forename: Seung Jin
authors.initials: SJ
authors.lastname: Lee
authors.affiliation: College of Natural Resources & Life Sciences, Pusan National University, Yangsan, Gyeongnam, Republic of Korea.
authors.forename: Young Whan
authors.initials: YW
authors.lastname: Choi
authors.affiliation: Department of Pharmacology, School of Medicine and MRC for Ischemic Tissue Regeneration, Pusan National University, Yangsan, Gyeongnam, Republic of Korea.
authors.forename: So Youn
authors.initials: SY
authors.lastname: Park
authors.affiliation: Department of Pharmacology, School of Medicine and MRC for Ischemic Tissue Regeneration, Pusan National University, Yangsan, Gyeongnam, Republic of Korea.
authors.forename: Chi Dae
authors.initials: CD
authors.lastname: Kim
mesh_terms: D000804:Angiotensin II / Q000009:adverse effects*; D000818:Animals; D000959:Antihypertensive Agents / Q000494:pharmacology / Q000627:therapeutic use; D001682:Biological Availability; D001794:Blood Pressure / Q000187:drug effects / Q000502:physiology; D034242:Cyclooctanes / Q000494:pharmacology / Q000627:therapeutic use; D004149:Dioxoles / Q000494:pharmacology / Q000627:therapeutic use; D004195:Disease Models, Animal; D006973:Hypertension / Q000139:chemically induced* / Q000503:physiopathology / Q000517:prevention & control*; D017705:Lignans / Q000494:pharmacology / Q000627:therapeutic use*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009569:Nitric Oxide / Q000378:metabolism*; D052250:Nitric Oxide Synthase Type III / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use*; D011083:Polycyclic Compounds / Q000494:pharmacology / Q000627:therapeutic use*; D017382:Reactive Oxygen Species / Q000378:metabolism; D032141:Schisandra; D016896:Treatment Outcome; D014665:Vasodilator Agents / Q000494:pharmacology / Q000627:therapeutic use
chemical_list: D000959:Antihypertensive Agents; D034242:Cyclooctanes; D004149:Dioxoles; D017705:Lignans; D010936:Plant Extracts; D011083:Polycyclic Compounds; D017382:Reactive Oxygen Species; D014665:Vasodilator Agents; D000804:Angiotensin II; D009569:Nitric Oxide; C033585:schizandrol B; D052250:Nitric Oxide Synthase Type III; C082091:gomisin J