title: Salinomycin inhibits the tumor growth of glioma stem cells by selectively suppressing glioma-initiating cells.
abstract: Glioma‑initiating cells are a small population of cells that have the ability to undergo self‑renewal and initiate tumorigenesis. In the present study, the potential role of salinomycin, a polyether antibiotic, on the suppression of glioma cell growth was investigated. GL261 glioma cells were maintained in a stem‑cell‑like status [GL261 neurospheres (GL261‑NS)] or induced for differentiation [GL261 adherent cells (GL261‑AC)]. It was demonstrated that salinomycin significantly reduced the cell viability of GL261‑NS and GL261‑AC cells in a dose‑dependent manner, with a more substantial inhibition of GL261‑NS proliferation (P<0.05). The inhibitory effect of salinomycin on cell growth was more effective than that of 1‑(4‑amino‑2‑methyl‑5‑pyrimid l)‑methyl‑3‑(2‑chloroethyl)‑3‑nitrosourea hydrochloride and vincristine (P<0.05). Salinomycin depleted GL261‑NS from tumorspheres and induced cell apoptosis. In addition, salinomycin prolonged the median survival time of glioma‑bearing mice (P<0.05). Therefore, the present study indicated that salinomycin may preferentially inhibit glioma‑initiated cell growth by inducing apoptosis, suggesting that salinomycin may provide a valuable therapeutic strategy for the treatment of malignant glioma.
authors.affiliation: Department of Neurosurgery, Southwest Hospital, Chongqing 400038, P.R. China.
authors.forename: Tunan
authors.initials: T
authors.lastname: Chen
authors.affiliation: Department of Neurosurgery, Daping Hospital, Chongqing 400042, P.R. China.
authors.forename: Liang
authors.initials: L
authors.lastname: Yi
authors.affiliation: Department of Neurosurgery, Southwest Hospital, Chongqing 400038, P.R. China.
authors.forename: Fei
authors.initials: F
authors.lastname: Li
authors.affiliation: Department of Neurosurgery, Southwest Hospital, Chongqing 400038, P.R. China.
authors.forename: Rong
authors.initials: R
authors.lastname: Hu
authors.affiliation: Department of Neurosurgery, Southwest Hospital, Chongqing 400038, P.R. China.
authors.forename: Shengli
authors.initials: S
authors.lastname: Hu
authors.affiliation: Department of Neurosurgery, Southwest Hospital, Chongqing 400038, P.R. China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Yin
authors.affiliation: Department of Neurosurgery, Southwest Hospital, Chongqing 400038, P.R. China.
authors.forename: Chuan
authors.initials: C
authors.lastname: Lan
authors.affiliation: Department of Neurosurgery, Southwest Hospital, Chongqing 400038, P.R. China.
authors.forename: Zhao
authors.initials: Z
authors.lastname: Li
authors.affiliation: Department of Neurosurgery, Southwest Hospital, Chongqing 400038, P.R. China.
authors.forename: Chuhua
authors.initials: C
authors.lastname: Fu
authors.affiliation: Department of Neurosurgery, Southwest Hospital, Chongqing 400038, P.R. China.
authors.forename: Liu
authors.initials: L
authors.lastname: Cao
authors.affiliation: Department of Neurosurgery, Southwest Hospital, Chongqing 400038, P.R. China.
authors.forename: Zhi
authors.initials: Z
authors.lastname: Chen
authors.affiliation: Department of Neurosurgery, Southwest Hospital, Chongqing 400038, P.R. China.
authors.forename: Jishu
authors.initials: J
authors.lastname: Xian
authors.affiliation: Department of Neurosurgery, Southwest Hospital, Chongqing 400038, P.R. China.
authors.forename: Hua
authors.initials: H
authors.lastname: Feng
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D045744:Cell Line, Tumor; D002470:Cell Survival; D004195:Disease Models, Animal; D005910:Glioma / Q000378:metabolism* / Q000401:mortality / Q000473:pathology*; D008297:Male; D051379:Mice; D014411:Neoplastic Stem Cells / Q000187:drug effects* / Q000378:metabolism*; D011714:Pyrans / Q000494:pharmacology*; D014410:Tumor Stem Cell Assay; D000095662:Polyether Polyketides
chemical_list: D000970:Antineoplastic Agents; D011714:Pyrans; C010327:salinomycin; D000095662:Polyether Polyketides