title: Synergistic protective role of mirazid (Commiphora molmol) and ascorbic acid against tilmicosin-induced cardiotoxicity in mice.
abstract: Tilmicosin (TIL) is a long-acting macrolide antibiotic approved for the treatment of cattle with Bovine Respiratory Disease. However, overdose of TIL has been reported to induce cardiotoxicity. The purpose of our experiment was to evaluate the protective effects of Commiphora molmol (mirazid (MRZ); myrrh) and (or) ascorbic acid (AA) against TIL-induced cardiotoxicity in mice. MRZ and AA were orally administered using stomach gavage, either alone or in combination for 5 consecutive days, followed with a single TIL overdose. TIL overdose induced a significant increase in serum levels of cardiac damage biomarkers (AST, LDH, CK, CK-MB, and cTnT), as well as cardiac lipid peroxidation, but cardiac levels of antioxidant biomarkers (GSH, SOD, CAT, and TAC) were decreased. Both MRZ and AA tended to normalize the elevated serum levels of cardiac injury biomarkers. Furthermore, MRZ and AA reduced TIL-induced lipid peroxidation and oxidative stress parameters. MRZ and AA combined produced a synergistic cardioprotective effect. We conclude that myrrh and (or) vitamin C administration minimizes the toxic effects of TIL through their free-radical-scavenging and potent antioxidant activities.
authors.affiliation: a Pharmacology Department, Faculty of Veterinary Medicine, Suez Canal University, Ismailia 41522, Egypt.
authors.forename: Mohamed M
authors.initials: MM
authors.lastname: Abdel-Daim
authors.forename: Emad W
authors.initials: EW
authors.lastname: Ghazy
authors.forename: Mostafa
authors.initials: M
authors.lastname: Fayez
mesh_terms: D000818:Animals; D001205:Ascorbic Acid / Q000008:administration & dosage*; D002316:Cardiotonic Agents / Q000008:administration & dosage*; D066126:Cardiotoxicity / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control; D029744:Commiphora; D004357:Drug Synergism; D015227:Lipid Peroxidation / Q000187:drug effects / Q000502:physiology; D008297:Male; D051379:Mice; D009206:Myocardium / Q000378:metabolism* / Q000473:pathology; D018384:Oxidative Stress / Q000187:drug effects / Q000502:physiology; D012116:Resins, Plant / Q000008:administration & dosage*; D015645:Tylosin / Q000031:analogs & derivatives* / Q000037:antagonists & inhibitors / Q000633:toxicity
chemical_list: D002316:Cardiotonic Agents; D012116:Resins, Plant; C587573:myrrh resin; D001205:Ascorbic Acid; C052319:tilmicosin; D015645:Tylosin
keywords: cardioprotecteur; cardioprotective; macrolide; myrrh; myrrhe; oxidative stress; stress oxydant; vitamin C; vitamine C