title: Pharmacological and safety evaluation of fibrous root of Rhizoma Coptidis.
abstract: The aim of this study was to investigated the pharmacological activities and safety of fibrous root of Rhizoma Coptidis (FRC). FRC not only protected Kunming mice from the minimal lethal dose of Escherichia coli, but also protected rabbits from hyperpyrexia induced by lipopolysaccharid (LPS). The acute toxicity study showed that oral medial lethal dose (LD50) of FRC was greater than 7000mg/kg body weight in Kunming mice. The sub-chronic toxicity study showed that the no-observed-adverse effect level (NOAEL) of FRC was 1.88g/kg body weight in Sprague-Dawley rats, whereas FRC at higher dose (3.76g/kg body weight) resulted in damage to liver and lung. Negative results were present in Ames test, mouse micronucleus test and mouse sperm abnormality test. These finding support the use of FRC in veterinary medicine.
authors.affiliation: School of Pharmaceutical Sciences, Southwest University, Chongqing 400716, China; Department of Pharmacy, Tongren Polytechnic College, Tongren 554300, Guizhou, China.
authors.forename: Na
authors.initials: N
authors.lastname: Ning
authors.affiliation: School of Pharmaceutical Sciences, Southwest University, Chongqing 400716, China.
authors.forename: Yan Zhi
authors.initials: YZ
authors.lastname: Wang
authors.affiliation: School of Pharmaceutical Sciences, Southwest University, Chongqing 400716, China.
authors.forename: Zong Yao
authors.initials: ZY
authors.lastname: Zou
authors.affiliation: School of Pharmaceutical Sciences, Southwest University, Chongqing 400716, China.
authors.forename: De Zhou
authors.initials: DZ
authors.lastname: Zhang
authors.affiliation: School of Pharmaceutical Sciences, Southwest University, Chongqing 400716, China.
authors.forename: De Zhen
authors.initials: DZ
authors.lastname: Wang
authors.affiliation: School of Pharmaceutical Sciences, Southwest University, Chongqing 400716, China. Electronic address: xuegangli2000@163.com.
authors.forename: Xue Gang
authors.initials: XG
authors.lastname: Li
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000627:therapeutic use / Q000633:toxicity; D058633:Antipyretics / Q000627:therapeutic use / Q000633:toxicity; D039323:Coptis; D004927:Escherichia coli Infections / Q000188:drug therapy; D005260:Female; D005334:Fever / Q000188:drug therapy; D007928:Lethal Dose 50; D008070:Lipopolysaccharides; D008297:Male; D008813:Mice, Inbred ICR; D009152:Mutagenicity Tests; D019055:No-Observed-Adverse-Effect Level; D008517:Phytotherapy; D010936:Plant Extracts / Q000627:therapeutic use / Q000633:toxicity; D018517:Plant Roots; D011817:Rabbits; D017207:Rats, Sprague-Dawley; D013094:Spermatozoa / Q000187:drug effects; D059550:Toxicity Tests, Subchronic
chemical_list: D000900:Anti-Bacterial Agents; D058633:Antipyretics; D008070:Lipopolysaccharides; D010936:Plant Extracts
keywords: Acute toxicity; Fibrous root; Mutagenicity; Pharmacological activities; Rhizoma Coptidis; Sub-chronic toxicity