title: Novel integrated and portable endotoxin detection system based on an electrochemical biosensor.
abstract: This paper describes the design, implementation and validation of a sensitive and integral technology solution for endotoxin detection. The unified and portable platform is based on the electrochemical detection of endotoxins using a synthetic peptide immobilized on a thin-film biosensor. The work covers the fabrication of an optimized sensor, the biofunctionalization protocol and the design and implementation of the measuring and signalling elements (a microfluidic chamber and a portable potentiostat-galvanostat), framed ad hoc for this specific application. The use of thin-film technologies to fabricate the biosensing device and the application of simple immobilization and detection methods enable a rapid, easy and sensitive technique for in situ and real time LPS detection.
authors.affiliation: CEIT and Tecnun (University of Navarra), Paseo Manuel Lardizábal N° 15, 20018 San Sebastián, Spain. a.zuzuarregui@nanogune.eu.
authors.forename: Ana
authors.initials: A
authors.lastname: Zuzuarregui
authors.forename: David
authors.initials: D
authors.lastname: Souto
authors.forename: Eva
authors.initials: E
authors.lastname: Pérez-Lorenzo
authors.forename: Fernando
authors.initials: F
authors.lastname: Arizti
authors.forename: Susana
authors.initials: S
authors.lastname: Sánchez-Gómez
authors.forename: Guillermo
authors.initials: G
authors.lastname: Martínez de Tejada
authors.forename: Klaus
authors.initials: K
authors.lastname: Brandenburg
authors.forename: Sergio
authors.initials: S
authors.lastname: Arana
authors.forename: Maite
authors.initials: M
authors.lastname: Mujika
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D055664:Electrochemical Techniques / Q000379:methods*; D004566:Electrodes; D004926:Escherichia coli / Q000472:pathogenicity; D008070:Lipopolysaccharides / Q000032:analysis*; D046210:Microfluidic Analytical Techniques / Q000379:methods
chemical_list: D008070:Lipopolysaccharides