title: Polyamine stimulation of eEF1A synthesis based on the unusual position of a complementary sequence to 18S rRNA in eEF1A mRNA.
abstract: It is thought that Shine-Dalgarno-like sequences, which exhibit complementarity to the nucleotide sequences at the 3'-end of 18S rRNA, are not present in eukaryotic mRNAs. However, complementary sequences consisting of more than 5 nucleotides to the 3'-end of 18S rRNA, i.e., a CR sequence, are present at -17 to -32 upstream from the initiation codon AUG in 18 mRNAs involved in protein synthesis except eEF1A mRNA. Thus, effects of the CR sequence in mRNAs and polyamines on protein synthesis were examined using control and polyamine-reduced FM3A and NIH3T3 cells. Polyamines did not stimulate protein synthesis encoded by 18 mRNAs possessing a normal CR sequence. When the CR sequence was deleted, protein synthetic activities decreased to less than 70% of intact mRNAs. In eEF1A mRNA, the CR sequence was located at -33 to -39 upstream from the initiation codon AUG, and polyamines stimulated eEF1A synthesis about threefold. When the CR sequence was shifted to -22 to -28 upstream from the AUG, eEF1A synthesis increased in polyamine-reduced cells and the degree of polyamine stimulation decreased greatly. The results indicate that the CR sequence exists in many eukaryotic mRNAs, and the location of a CR sequence in mRNAs influences polyamine stimulation of protein synthesis.
authors.affiliation: Faculty of Pharmacy, Chiba Institute of Science, 15-8 Shiomi-cho, Choshi, Chiba, 288-0025, Japan.
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Terui
authors.forename: Akihiko
authors.initials: A
authors.lastname: Sakamoto
authors.forename: Taketo
authors.initials: T
authors.lastname: Yoshida
authors.forename: Takuma
authors.initials: T
authors.lastname: Kasahara
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Tomitori
authors.forename: Kyohei
authors.initials: K
authors.lastname: Higashi
authors.forename: Kazuei
authors.initials: K
authors.lastname: Igarashi
authors.forename: Keiko
authors.initials: K
authors.lastname: Kashiwagi
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D018387:Codon, Initiator / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D020648:Peptide Elongation Factor 1 / Q000096:biosynthesis* / Q000235:genetics; D011073:Polyamines / Q000494:pharmacology*; D014176:Protein Biosynthesis / Q000187:drug effects* / Q000502:physiology; D012337:RNA, Ribosomal, 18S / Q000235:genetics / Q000378:metabolism*
chemical_list: D018387:Codon, Initiator; D020648:Peptide Elongation Factor 1; D011073:Polyamines; D012337:RNA, Ribosomal, 18S