title: Niosomes based on synthetic cationic lipids for gene delivery: the influence of polar head-groups on the transfection efficiency in HEK-293, ARPE-19 and MSC-D1 cells.
abstract: We designed niosomes based on three lipids that differed only in the polar-head group to analyze their influence on the transfection efficiency. These lipids were characterized by small-angle X-ray scattering before being incorporated into the niosomes which were characterized in terms of pKa, size, zeta potential, morphology and physical stability. Nioplexes were obtained upon the addition of a plasmid. Different ratios (w/w) were selected to analyze the influence of this parameter on size, charge and the ability to condense, release and protect the DNA. In vitro transfection experiments were performed in HEK-293, ARPE-19 and MSC-D1 cells. Our results show that the chemical composition of the cationic head-group clearly affects the physicochemical parameters of the niosomes and especially the transfection efficiency. Only niosomes based on cationic lipids with a dimethyl amino head group (lipid 3) showed a transfection capacity when compared with their counterparts amino (lipid 1) and tripeptide head-groups (lipid 2). Regarding cell viability, we clearly observed that nioplexes based on the cationic lipid 3 had a more deleterious effect than their counterparts, especially in ARPE-19 cells at 20/1 and 30/1 ratios. Similar studies could be extended to other series of cationic lipids in order to progress in the research on safe and efficient non-viral vectors for gene delivery purposes.
authors.affiliation: NanoBioCel Group, University of Basque Country, Vitoria, Spain. joseluis.pedraz@ehu.es.
authors.forename: E
authors.initials: E
authors.lastname: Ojeda
authors.forename: G
authors.initials: G
authors.lastname: Puras
authors.forename: M
authors.initials: M
authors.lastname: Agirre
authors.forename: J
authors.initials: J
authors.lastname: Zárate
authors.forename: S
authors.initials: S
authors.lastname: Grijalvo
authors.forename: R
authors.initials: R
authors.lastname: Pons
authors.forename: R
authors.initials: R
authors.lastname: Eritja
authors.forename: G
authors.initials: G
authors.lastname: Martinez-Navarrete
authors.forename: C
authors.initials: C
authors.lastname: Soto-Sanchez
authors.forename: E
authors.initials: E
authors.lastname: Fernández
authors.forename: J L
authors.initials: JL
authors.lastname: Pedraz
mesh_terms: D002470:Cell Survival / Q000187:drug effects; D004247:DNA / Q000008:administration & dosage / Q000737:chemistry / Q000235:genetics; D004355:Drug Stability; D057809:HEK293 Cells; D006801:Humans; D008055:Lipids / Q000138:chemical synthesis / Q000737:chemistry* / Q000633:toxicity; D008081:Liposomes; D010316:Particle Size; D014162:Transfection
chemical_list: D008055:Lipids; D008081:Liposomes; D004247:DNA