title: The inflammation and estrogen metabolism impacts of polychlorinated biphenyls on endometrial cancer cells.
abstract: Polychlorinated biphenyls (PCBs) are persistent and bio-accumulative chemicals that provoke a wide range of toxic effects. Their adverse impacts on the reproductive system are of great concern, however, the effects of PCBs on endometrium are still unclear. In the study, the endometrial adenocarcinoma Ishikawa cells were exposed to both dioxin-like CB126 and non-dioxin-like CB153 at the nominal concentrations of 0.3, 3, and 30μM. The inflammatory and endocrine effects were detected after treatment by PCBs. Results showed that CB126 stimulated the proliferation of Ishikawa cells at lower concentrations of 0.3 and 3μM. By contrast, CB153 did not affect the viability of the cells. Both congeners exerted the stimulatory effects on the enzymatic activity of SOD1. CB126 decreased the abundance of Interleukin-8 both at the mRNA and protein levels. Blocking of estrogen receptor or aryl hydrocarbon receptor by the antagonist abolished the effects of CB126 on the expressions of inflammatory factors. The levels of testosterone and 17beta-estradiol were not changed after exposure to lower doses of PCBs. In accordance, PCBs did not affect the mRNA expressions of estrogen metabolism-related genes. In all, our study revealed that PCBs affected the expression of inflammatory factors through ER and AHR receptors, however, no toxic effects were observed on estrogen metabolism.
authors.affiliation: Key Lab of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, PR China; Ningbo Urban Environment Observation and Research Station-NUEORS, Chinese Academy of Sciences, PR China.
authors.forename: Yajie
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Key Lab of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, PR China; Ningbo Urban Environment Observation and Research Station-NUEORS, Chinese Academy of Sciences, PR China. Electronic address: qshuang@iue.ac.cn.
authors.forename: Qiansheng
authors.initials: Q
authors.lastname: Huang
authors.affiliation: First Affiliated Hospital of Xiamen University, Xiamen 361003, PR China.
authors.forename: Qionghua
authors.initials: Q
authors.lastname: Chen
authors.affiliation: Key Lab of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, PR China; Ningbo Urban Environment Observation and Research Station-NUEORS, Chinese Academy of Sciences, PR China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Lin
authors.affiliation: Key Lab of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, PR China; Ningbo Urban Environment Observation and Research Station-NUEORS, Chinese Academy of Sciences, PR China.
authors.forename: Xia
authors.initials: X
authors.lastname: Sun
authors.affiliation: Key Lab of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, PR China; Ningbo Urban Environment Observation and Research Station-NUEORS, Chinese Academy of Sciences, PR China.
authors.forename: Huanteng
authors.initials: H
authors.lastname: Zhang
authors.affiliation: First Affiliated Hospital of Xiamen University, Xiamen 361003, PR China.
authors.forename: Maobi
authors.initials: M
authors.lastname: Zhu
authors.affiliation: Key Lab of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, PR China; Ningbo Urban Environment Observation and Research Station-NUEORS, Chinese Academy of Sciences, PR China. Electronic address: sjdong@iue.ac.cn.
authors.forename: Sijun
authors.initials: S
authors.lastname: Dong
mesh_terms: D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D019363:Cytochrome P-450 CYP1A1 / Q000235:genetics; D016889:Endometrial Neoplasms / Q000378:metabolism*; D004958:Estradiol / Q000378:metabolism; D004965:Estrogen Antagonists / Q000633:toxicity; D004967:Estrogens / Q000378:metabolism*; D005260:Female; D006801:Humans; D007249:Inflammation / Q000378:metabolism*; D016209:Interleukin-8 / Q000235:genetics / Q000378:metabolism; D011078:Polychlorinated Biphenyls / Q000633:toxicity*; D012333:RNA, Messenger / Q000378:metabolism; D018336:Receptors, Aryl Hydrocarbon / Q000037:antagonists & inhibitors / Q000378:metabolism; D011960:Receptors, Estrogen / Q000037:antagonists & inhibitors / Q000378:metabolism; D013482:Superoxide Dismutase / Q000235:genetics / Q000378:metabolism; D000072105:Superoxide Dismutase-1; D013739:Testosterone / Q000378:metabolism
chemical_list: C508606:CXCL8 protein, human; D004965:Estrogen Antagonists; D004967:Estrogens; D016209:Interleukin-8; D012333:RNA, Messenger; D018336:Receptors, Aryl Hydrocarbon; D011960:Receptors, Estrogen; C000606290:SOD1 protein, human; D013739:Testosterone; D004958:Estradiol; D011078:Polychlorinated Biphenyls; C569463:CYP1A1 protein, human; D019363:Cytochrome P-450 CYP1A1; D013482:Superoxide Dismutase; D000072105:Superoxide Dismutase-1; C023035:3,4,5,3',4'-pentachlorobiphenyl; C014024:2,4,5,2',4',5'-hexachlorobiphenyl
keywords: CB126; Endocrine; Estrogen metabolism; Inflammation; Ishikawa cells; Polychlorinated biphenyls