title: A high-throughput metabolomic approach to explore the regulatory effect of mangiferin on metabolic network disturbances of hyperlipidemia rats.
abstract: This paper was designed to study metabolomic characters of the high-fat diet (HFD)-induced hyperlipidemia and the intervention effects of Mangiferin (MG). In this study, we aimed to investigate the intervention of MG on rats with hyperlipidemia induced by HFD and explore the possible mechanisms of hyperlipidemia. Urine metabolic profiles were analyzed using ultra-performance liquid chromatography/electrospray ionization quadruple time-of-flight mass spectrometry (UPLC-ESI-QTOF-MS) coupled with the principal component analysis (PCA) and partial least squares-discriminate analysis (PLS-DA) models, Heatmap and metabolism pathway analysis. PCA was applied to study the trajectory of the urinary metabolic phenotype of hyperlipidemia rat after administration of MG. The VIP-plot of orthogonal PLS-DA was used for discovering potential biomarkers to clarify the mechanism of MG. Biochemical analyses indicate that MG can alleviate the hyperlipidemia damage. Twenty significantly changed metabolites (potential biomarkers) were found to be reasonable in explaining the action mechanism of MG. The effectiveness of MG on hyperlipidemia is proved using the established metabolomic method and the regulated metabolic pathways involve the TCA cycle, taurine and hypotaurine metabolism, glyoxylate and dicarboxylate metabolism, glycine and serine and threonine metabolism, glycerophospholipid metabolism, primary bile acid biosynthesis etc. The results indicated that MG has a favourable protective effect on HFD-induced hyperlipidemia by adjusting the metabolic disorders. It also suggests that the metabolomic technology is a powerful approach for elucidation of the action mechanisms of MG.
authors.affiliation: Development and Utilization Key Laboratory of Northeast Plant Materials of Liaoning Province, Department of Pharmacognosy, School of Traditional Chinese Materia Medica 48#, Shenyang Pharmaceutical University, 103 Wenhua Road, Shenhe District, Shenyang 110016, China. junyin_good@163.com 1628394118@qq.com.
authors.forename: Chengyan
authors.initials: C
authors.lastname: Zhou
authors.forename: Gang
authors.initials: G
authors.lastname: Li
authors.forename: Yanchuan
authors.initials: Y
authors.lastname: Li
authors.forename: Liya
authors.initials: L
authors.lastname: Gong
authors.forename: Yifan
authors.initials: Y
authors.lastname: Huang
authors.forename: Zhiping
authors.initials: Z
authors.lastname: Shi
authors.forename: Shanshan
authors.initials: S
authors.lastname: Du
authors.forename: Ying
authors.initials: Y
authors.lastname: Li
authors.forename: Maoqing
authors.initials: M
authors.lastname: Wang
authors.forename: Jun
authors.initials: J
authors.lastname: Yin
authors.forename: Changhao
authors.initials: C
authors.lastname: Sun
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D002784:Cholesterol / Q000378:metabolism; D002853:Chromatography, Liquid; D016002:Discriminant Analysis; D057166:High-Throughput Screening Assays / Q000379:methods*; D006949:Hyperlipidemias / Q000378:metabolism*; D016018:Least-Squares Analysis; D050356:Lipid Metabolism / Q000187:drug effects; D008099:Liver / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D008297:Male; D053858:Metabolic Networks and Pathways / Q000187:drug effects*; D055442:Metabolome / Q000187:drug effects; D055432:Metabolomics / Q000379:methods*; D017207:Rats, Sprague-Dawley; D021241:Spectrometry, Mass, Electrospray Ionization; D013654:Taurine / Q000032:analysis; D014280:Triglycerides / Q000378:metabolism; D044004:Xanthones / Q000494:pharmacology*
chemical_list: D015415:Biomarkers; D014280:Triglycerides; D044004:Xanthones; D013654:Taurine; C013592:mangiferin; D002784:Cholesterol