title: CC genotype at rs12979860 of IL28B is associated with lower risk of new-onset diabetes after transplantation in adult patients with liver transplantation for hepatitis C cirrhosis.
abstract: BACKGROUND
  New-onset diabetes mellitus after transplantation (NODAT) in patients undergoing liver transplantation (LT) for hepatitis C virus (HCV)-related cirrhosis is associated with more aggressive HCV recurrence on the graft, rapid progression of fibrosis, and lower rate of sustained viral response to antiviral therapy. The CC genotype at rs12979860 of the IL28B is associated with greater rates of spontaneous clearance of HCV and response to antiviral therapy. IL28B acts on the interferon-stimulated genes through the JAK-STAT pathway, which is related to the development of insulin resistance. The aim of this study was to investigate whether IL28B rs12979860 polymorphism is associated with the development of NODAT after LT for cirrhosis owing to HCV infection.
  
  
  METHODS
  We analyzed 99 patients (age, 52.7 ± 9.4 years; 70% male) who underwent LT for HCV-related cirrhosis, with ≥1 year of follow-up and with available DNA sample. NODAT was defined starting from the sixth month after LT, according to the international consensus guidelines. Genotyping was carried out by real-time polymerase chain reaction and analysis of the melting temperature with the LightCycler 480 system.
  
  
  RESULTS
  Twenty-eight patients (28.3%) developed NODAT. CC genotype at rs12979860 of IL28B was associated with a lesser incidence of NODAT versus non-CC genotypes (P = .05; odds ratio, 0.31; 95% CI, 0.11-0.92). We did not find any association between NODAT and age at transplantation, gender, pretransplant body mass index, presence of hepatocellular carcinoma, type of initial immunosuppression (cyclosporine, tacrolimus or corticosteroids) or acute rejection treated with steroids.
  
  
  CONCLUSIONS
  The CC genotype at rs12979860 of IL28B is a protective factor for NODAT in patients with LT for HCV-related cirrhosis.
authors.affiliation: Unidad de Trasplante Hepático, Hospital Universitario Puerta de Hierro Majadahonda, Madrid, Spain. Electronic address: ani_duca@yahoo.com.
authors.forename: A M
authors.initials: AM
authors.lastname: Duca
authors.affiliation: Departamento de Medicina Interna, Hospital Universitario Puerta de Hierro Majadahonda (IDIPHIM), Madrid, Spain.
authors.forename: S
authors.initials: S
authors.lastname: de la Fuente
authors.affiliation: Departamento de Medicina Interna, Hospital Universitario Puerta de Hierro Majadahonda (IDIPHIM), Madrid, Spain.
authors.forename: M J
authors.initials: MJ
authors.lastname: Citores
authors.affiliation: Departamento de Medicina Interna, Hospital Universitario Puerta de Hierro Majadahonda (IDIPHIM), Madrid, Spain.
authors.forename: A B
authors.initials: AB
authors.lastname: Cuenca
authors.affiliation: Laboratorio de Inmunogenética e Histocompatibilidad, Hospital Universitario Puerta de Hierro Majadahonda, Madrid, Spain.
authors.forename: E
authors.initials: E
authors.lastname: Cisneros
authors.affiliation: Unidad de Trasplante Hepático, Hospital Universitario Puerta de Hierro Majadahonda, Madrid, Spain.
authors.forename: N
authors.initials: N
authors.lastname: Escamilla
authors.affiliation: Unidad de Trasplante Hepático, Hospital Universitario Puerta de Hierro Majadahonda, Madrid, Spain.
authors.forename: I
authors.initials: I
authors.lastname: Baños
authors.affiliation: Laboratorio de Inmunogenética e Histocompatibilidad, Hospital Universitario Puerta de Hierro Majadahonda, Madrid, Spain.
authors.forename: C
authors.initials: C
authors.lastname: Vilches
authors.affiliation: Unidad de Trasplante Hepático, Hospital Universitario Puerta de Hierro Majadahonda, Madrid, Spain.
authors.forename: V
authors.initials: V
authors.lastname: Cuervas-Mons
mesh_terms: D000328:Adult; D000368:Aged; D003920:Diabetes Mellitus / Q000235:genetics*; D005260:Female; D005838:Genotype; D006526:Hepatitis C / Q000150:complications; D006801:Humans; D007333:Insulin Resistance / Q000235:genetics; D007372:Interferons; D007378:Interleukins / Q000235:genetics*; D008103:Liver Cirrhosis / Q000601:surgery / Q000821:virology; D016031:Liver Transplantation / Q000009:adverse effects; D008297:Male; D008875:Middle Aged; D009364:Neoplasm Recurrence, Local; D011110:Polymorphism, Genetic; D060888:Real-Time Polymerase Chain Reaction; D012189:Retrospective Studies; D000095022:Interferon Lambda
chemical_list: D007372:Interferons; D007378:Interleukins; C000624353:interferon-lambda, human; D000095022:Interferon Lambda