title: Modulation of phagolysosome maturation by bacterial tlyA gene product.
abstract: The pathogenic traits of TlyA proteins of Mycobacterium tuberculosis are not known. Expressions of TlyA in bacteria that do not express endogenous TlyA adhere better to RAW264.7 macrophages and get phagocytosed efficiently. The internalized bacteria avoid acidification to the extent of greater than 65 percent in the case of both TlyA-expressing E. coli and M. smegmatis. Consistent with this observation, we have observed decreased co-localizaton of Lysosomal Membrane Associated Protein-1 (approx. 35 percent), Early Endosomal Antigen-1 (approx. 34 percent), Rab5 (approx. 30 percent) and Rab7 (approx. 35 percent) and enhanced colocalizaton of Rab14 (approx. 80 percent) on both TlyA-expressing bacteria as well as on TlyA-coated latex beads. These results suggest that the mycobacterial TlyA, in general, can modulate phagolysosome maturation pathway immediately after entry into macrophages, while other important molecules may aid the bacterium for long-term, intracellular survival at later point of time.
authors.affiliation: National Centre for Cell Science, Ganeshkhind Road, Pune University Campus, Pune 411 007, India.
authors.forename: Ekansh
authors.initials: E
authors.lastname: Mittal
authors.forename: Santosh
authors.initials: S
authors.lastname: Kumar
authors.forename: Aejazur
authors.initials: A
authors.lastname: Rahman
authors.forename: Musti V
authors.initials: MV
authors.lastname: Krishnasastry
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D004926:Escherichia coli / Q000235:genetics; D051379:Mice; D008810:Mice, Inbred C57BL; D020102:Mycobacterium smegmatis / Q000235:genetics / Q000472:pathogenicity; D010587:Phagocytosis; D010588:Phagosomes / Q000502:physiology*; D037521:Virulence Factors / Q000235:genetics / Q000378:metabolism / Q000502:physiology*
chemical_list: D001426:Bacterial Proteins; D037521:Virulence Factors