title: Intrinsic apoptosis pathway in fallopian tube epithelial cells induced by cladribine.
abstract: Cladribine is a purine nucleoside analog which initiates the apoptotic mechanism within cells. Moreover, the available data confirms that cladribine, with the participation of the p53 protein, as well as the proapoptotic proteins from the Bcl-2 family, also induces the activation of the intrinsic apoptosis pathway. However, while there has been a lot of research devoted to the effect of cladribine on lymphatic system cells, little is known about the impact of cladribine on the reproductive system. The aim of our study was to evaluate apoptosis in oviduct epithelial cells sourced from 15 different female rats. In so doing, the sections were stained with caspases 3, 9, and 8. Results suggest that cladribine also induces apoptosis in the oviduct epithelial cells by way of the intrinsic pathway. Indeed, the discontinuing of the administration of cladribine leads to a reduction in the amount of apoptotic cells in the oviduct epithelium.
authors.affiliation: Chair and Department of Histology and Embryology with Experimental Cytology Unit, Medical University of Lublin, Radziwiłłowska 11 Street, 20-080 Lublin, Poland.
authors.forename: Ewelina
authors.identifier: 0000-0001-6914-6735
authors.initials: E
authors.lastname: Wawryk-Gawda
authors.affiliation: Chair and Department of Histology and Embryology with Experimental Cytology Unit, Medical University of Lublin, Radziwiłłowska 11 Street, 20-080 Lublin, Poland.
authors.forename: Patrycja
authors.initials: P
authors.lastname: Chylińska-Wrzos
authors.affiliation: Chair and Department of Histology and Embryology with Experimental Cytology Unit, Medical University of Lublin, Radziwiłłowska 11 Street, 20-080 Lublin, Poland.
authors.forename: Marta
authors.initials: M
authors.lastname: Lis-Sochocka
authors.affiliation: Chair and Department of Histology and Embryology with Experimental Cytology Unit, Medical University of Lublin, Radziwiłłowska 11 Street, 20-080 Lublin, Poland.
authors.forename: Kamila
authors.initials: K
authors.lastname: Bulak
authors.affiliation: Chair and Department of Histology and Embryology with Experimental Cytology Unit, Medical University of Lublin, Radziwiłłowska 11 Street, 20-080 Lublin, Poland.
authors.forename: Barbara
authors.initials: B
authors.lastname: Jodłowska-Jędrych
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects* / Q000235:genetics; D053148:Caspase 3 / Q000235:genetics / Q000378:metabolism; D053181:Caspase 8 / Q000235:genetics / Q000378:metabolism; D053453:Caspase 9 / Q000235:genetics* / Q000378:metabolism; D002452:Cell Count; D017338:Cladribine / Q000494:pharmacology*; D004847:Epithelial Cells / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology; D030762:Estrous Cycle / Q000502:physiology; D005187:Fallopian Tubes / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology; D005260:Female; D015870:Gene Expression; D007279:Injections, Subcutaneous; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000970:Antineoplastic Agents; D017338:Cladribine; D053148:Caspase 3; D053181:Caspase 8; D053453:Caspase 9