title: High miR-449b expression in prostate cancer is associated with biochemical recurrence after radical prostatectomy.
abstract: BACKGROUND
  Prostate cancer is one of the leading causes of cancer death amongst men in economically advanced countries. The disease is characterized by a greatly varying clinical course, where some patients harbor non- or slowly-progressive disease, others highly aggressive disease. There is a great lack of markers to differentiate between aggressive and indolent disease. Markers that could help to identify patients needing curative treatment while sparing those who do not.
  
  
  METHODS
  MicroRNA profiling of 672 microRNAs using multiplex RT-qPCR was performed using 36 prostate cancer samples to evaluate the association of microRNAs and biochemical recurrence after radical prostatectomy.
  
  
  RESULTS
  Among 31 microRNAs associated with recurrence, we identified miR-449b, which was further validated in an independent cohort of 163 radical prostatectomy patients. Patients expressing miR-449b had a significantly higher risk of recurrence (HR = 1.57; p = 0.028), and miR-449b was shown to be an independent predictor of recurrence after prostatectomy (HR = 1.9; p = 0.003) when modeled with known risk factors of recurrent disease in multivariate analysis.
  
  
  CONCLUSIONS
  High miR-449b expression was shown to be an independent predictor of biochemical recurrence after radical prostatectomy.
authors.forename: Martin Mørck
authors.initials: MM
authors.lastname: Mortensen
authors.forename: Søren
authors.initials: S
authors.lastname: Høyer
authors.forename: Torben Falck
authors.initials: TF
authors.lastname: Orntoft
authors.forename: Karina Dalsgaard
authors.initials: KD
authors.lastname: Sørensen
authors.affiliation: Department of Molecular Medicine, Aarhus University Hospital, Brendstrupgaardsvej 100, 8200 Aarhus N, Denmark. lars@clin.au.dk.
authors.forename: Lars
authors.initials: L
authors.lastname: Dyrskjøt
authors.forename: Michael
authors.initials: M
authors.lastname: Borre
mesh_terms: D000368:Aged; D015415:Biomarkers; D015331:Cohort Studies; D015870:Gene Expression; D020869:Gene Expression Profiling; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D060787:Neoplasm Grading; D009367:Neoplasm Staging; D011468:Prostatectomy; D011471:Prostatic Neoplasms / Q000235:genetics* / Q000378:metabolism* / Q000401:mortality / Q000473:pathology / Q000601:surgery; D012008:Recurrence; D015203:Reproducibility of Results
chemical_list: D015415:Biomarkers; C544530:MIRN449 microRNA, human; D035683:MicroRNAs