title: A lectin from Dioclea violacea Interacts with midgut surface of Lutzomyia migonei, unlike its homologues, Cratylia floribunda lectin and Canavalia gladiata lectin.
abstract: Leishmaniasis is a vector-borne disease transmitted by phlebotomine sand fly. Susceptibility and refractoriness to Leishmania depend on the outcome of multiple interactions that take place within the sand fly gut. Promastigote attachment to sand fly midgut epithelium is essential to avoid being excreted together with the digested blood meal. Promastigote and gut sand fly surface glycans are important ligands in this attachment. The purpose of the present study was to evaluate the interaction of three lectins isolated from leguminous seeds (Diocleinae subtribe), D-glucose and D-mannose-binding, with glycans on Lutzomyia migonei midgut. To study this interaction the lectins were labeled with FITC and a fluorescence assay was performed. The results showed that only Dioclea violacea lectin (DVL) was able to interact with midgut glycans, unlike Cratylia floribunda lectin (CFL) and Canavalia gladiata lectin (CGL). Furthermore, when DVL was blocked with D-mannose the interaction was inhibited. Differences of spatial arrangement of residues and volume of carbohydrate recognition domain (CRD) may be the cause of the fine specificity of DVL for glycans in the surface on Lu. migonei midgut. The findings in this study showed the presence of glycans in the midgut with glucose/mannose residues in its composition and these residues may be important in interaction between Lu. migonei midgut and Leishmania.
authors.affiliation: Laboratory of Parasitology, Faculty of Medicine, Federal University of Ceará, 60430-160 Fortaleza, CE, Brazil.
authors.forename: Juliana Montezuma Barbosa
authors.initials: JM
authors.lastname: Monteiro Tínel
authors.affiliation: Laboratory of Parasitology, Faculty of Medicine, Federal University of Ceará, 60430-160 Fortaleza, CE, Brazil.
authors.forename: Melina Fechine Costa
authors.initials: MF
authors.lastname: Benevides
authors.affiliation: Laboratory of Parasitology, Faculty of Medicine, Federal University of Ceará, 60430-160 Fortaleza, CE, Brazil.
authors.forename: Mércia Sindeaux
authors.initials: MS
authors.lastname: Frutuoso
authors.affiliation: Laboratory of Parasitology, Faculty of Medicine, Federal University of Ceará, 60430-160 Fortaleza, CE, Brazil.
authors.forename: Camila Farias
authors.initials: CF
authors.lastname: Rocha
authors.affiliation: Integrated Laboratory of Biomolecules (LIBS), Department of Pathology and Legal Medicine, Faculty of Medicine, Federal University of Ceará, 60430-160 Fortaleza, CE, Brazil.
authors.forename: Francisco Vassiliepe Sousa
authors.initials: FV
authors.lastname: Arruda
authors.affiliation: Integrated Laboratory of Biomolecules (LIBS), Department of Pathology and Legal Medicine, Faculty of Medicine, Federal University of Ceará, 60430-160 Fortaleza, CE, Brazil.
authors.forename: Mayron Alves
authors.initials: MA
authors.lastname: Vasconcelos
authors.affiliation: Laboratory of Biologically Actives Molecules, Biochemistry and Molecular Biology Department, Federal University of Ceará, 60440-970 Fortaleza, CE, Brazil.
authors.forename: Francisco Nascimento
authors.initials: FN
authors.lastname: Pereira-Junior
authors.affiliation: Laboratory of Biologically Actives Molecules, Biochemistry and Molecular Biology Department, Federal University of Ceará, 60440-970 Fortaleza, CE, Brazil.
authors.forename: João Batista
authors.initials: JB
authors.lastname: Cajazeiras
authors.affiliation: Laboratory of Biologically Actives Molecules, Biochemistry and Molecular Biology Department, Federal University of Ceará, 60440-970 Fortaleza, CE, Brazil.
authors.forename: Kyria Santiago
authors.initials: KS
authors.lastname: do Nascimento
authors.affiliation: Institute of Chemical and Geosciences, Federal University of Pelotas Institute of Chemical and Geosciences, Federal University of Pelotas, 96160-000 Pelotas, RS, Brazil.
authors.forename: Jorge Luiz
authors.initials: JL
authors.lastname: Martins
authors.affiliation: Integrated Laboratory of Biomolecules (LIBS), Department of Pathology and Legal Medicine, Faculty of Medicine, Federal University of Ceará, 60430-160 Fortaleza, CE, Brazil.
authors.forename: Edson Holanda
authors.initials: EH
authors.lastname: Teixeira
authors.affiliation: Laboratory of Biologically Actives Molecules, Biochemistry and Molecular Biology Department, Federal University of Ceará, 60440-970 Fortaleza, CE, Brazil.
authors.forename: Benildo Sousa
authors.initials: BS
authors.lastname: Cavada
authors.affiliation: Computer Engineering of Sobral, Federal University of Ceará, 62042-280 Sobral, CE, Brazil.
authors.forename: Ricardo Pires
authors.initials: RP
authors.lastname: dos Santos
authors.affiliation: Laboratory of Parasitology, Faculty of Medicine, Federal University of Ceará, 60430-160 Fortaleza, CE, Brazil.
authors.forename: Margarida Maria
authors.initials: MM
authors.lastname: Lima Pompeu
mesh_terms: D000818:Animals; D032449:Canavalia; D052598:Dioclea; D065569:Gastrointestinal Absorption / Q000502:physiology*; D037102:Lectins / Q000302:isolation & purification / Q000378:metabolism; D037121:Plant Lectins / Q000302:isolation & purification / Q000378:metabolism*; D011576:Psychodidae; D012639:Seeds
chemical_list: D037102:Lectins; D037121:Plant Lectins; C039268:lectin, Canavalia