title: Hepatoprotective effects of sesamol loaded solid lipid nanoparticles in carbon tetrachloride induced sub-chronic hepatotoxicity in rats.
abstract: Sesamol is a phenolic component of sesame seed oil, which has been established as an antioxidant and also possesses potential for hepatoprotection. However, its protective role in carbon tetrachloride (CCl4 ) induced sub-chronic hepatotoxicity has not been studied. Limited oral bioavailability (BA) and rapid elimination (as conjugates) in rats is reported for sesamol. Considering its significant antioxidant potential and compromised BA, we packaged sesamol into solid lipid nanoparticles (S-SLNs) to enhance its hepatoprotective bioactivity. S-SLNs prepared by microemulsification method were nearly spherical in shape with an average particle size of 120.30 nm and their oral administration at 8 mg/kg body weight (BW) showed significantly (p < 0.001) better hepatoprotection than free sesamol (FS) and a well established hepatoprotective antioxidant silymarin [SILY (25 mg/kg BW); p < 0.05) in CCl4 induced sub-chronic liver injury in rats. Evaluations were done in terms of histological changes in the liver tissue, liver injury markers (serum alanine aminotransferase, serum aspartate aminotransferase, and serum lactate dehydrogenase); oxidative stress markers (lipid peroxidation, superoxide dismutase, and reduced glutathione) and proinflammatory response marker (tumor necrosis factor-alpha).
authors.affiliation: Department of Biotechnology, Panjab University, Chandigarh, 160014, India.
authors.forename: Neha
authors.initials: N
authors.lastname: Singh
authors.affiliation: Department of Biotechnology, Panjab University, Chandigarh, 160014, India.
authors.forename: Neeraj
authors.initials: N
authors.lastname: Khullar
authors.affiliation: Department of Pharmaceutics, University Institute of Pharmaceutical Sciences, Panjab University, Chandigarh, 160014, India.
authors.forename: Vandita
authors.initials: V
authors.lastname: Kakkar
authors.affiliation: Department of Pharmaceutics, University Institute of Pharmaceutical Sciences, Panjab University, Chandigarh, 160014, India.
authors.forename: Indu Pal
authors.initials: IP
authors.lastname: Kaur
mesh_terms: D000284:Administration, Oral; D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D052117:Benzodioxoles / Q000737:chemistry / Q000494:pharmacology*; D002251:Carbon Tetrachloride / Q000633:toxicity; D056486:Chemical and Drug Induced Liver Injury / Q000378:metabolism / Q000473:pathology* / Q000517:prevention & control; D005978:Glutathione / Q000378:metabolism; D006836:Hydro-Lyases / Q000097:blood; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000378:metabolism / Q000473:pathology; D008297:Male; D053758:Nanoparticles / Q000737:chemistry*; D009822:Oils, Volatile / Q000737:chemistry; D010316:Particle Size; D010636:Phenols / Q000737:chemistry / Q000494:pharmacology*; D020011:Protective Agents / Q000737:chemistry / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D052117:Benzodioxoles; D009822:Oils, Volatile; D010636:Phenols; D020011:Protective Agents; D014409:Tumor Necrosis Factor-alpha; C025583:sesamol; D002251:Carbon Tetrachloride; D013482:Superoxide Dismutase; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D006836:Hydro-Lyases; C045581:lactate dehydratase; D005978:Glutathione
keywords: CCl4; SLNs; hepatoprotective; oxidative stress; sesamol