title: Adhesion, growth, and proliferation of endothelial cells on biopolymer extracellular film matrices.
abstract: We analyzed the influence of initial properties of biopolymer fi lm extracellular matrices made of chitosan on adhesion of swine embryonic kidney epithelium-like cells, their morphology, growth, and proliferation. It was found that adhesion index of cells on the studied matrices was similar, but cell morphology had significant qualitative differences. Round non-flattened cells were described in endothelial cell cultures on chitosan-based matrices. Local variations in charge density on matrices induced by the presence of chitin nanofibers led to the appearance of flattened spindle-shaped and non-flattened sphericalal cells. Long-term culturing on biopolymer extracellular fi lm matrices was associated by the formation of spatial conglomerates of sphericalal cells.
authors.affiliation: Institute of Problems of Cryobiology and Cryomedicine, National Academy of Sciences of Ukraine, Khar'kov, Ukraine.
authors.forename: V V
authors.initials: VV
authors.lastname: Kiroshka
authors.forename: T A
authors.initials: TA
authors.lastname: Yurchuk
authors.forename: N V
authors.initials: NV
authors.lastname: Repin
authors.forename: V A
authors.initials: VA
authors.lastname: Petrova
authors.forename: I V
authors.initials: IV
authors.lastname: Gofman
authors.forename: Yu A
authors.initials: YA
authors.lastname: Skorik
authors.forename: E V
authors.initials: EV
authors.lastname: Kiroshka
authors.forename: T P
authors.initials: TP
authors.lastname: Bondarenko
mesh_terms: D000818:Animals; D002448:Cell Adhesion; D049109:Cell Proliferation; D048430:Cell Shape; D002478:Cells, Cultured; D048271:Chitosan / Q000737:chemistry; D042783:Endothelial Cells / Q000502:physiology*; D005109:Extracellular Matrix / Q000737:chemistry; D008567:Membranes, Artificial; D057139:Nanofibers / Q000737:chemistry; D034421:Sus scrofa; D023822:Tissue Engineering
chemical_list: D008567:Membranes, Artificial; D048271:Chitosan