title: [Amiloride reduces proteinuria and inhibits podocyte uPAR in the 5/6 nephrectomy rats].
abstract: OBJECTIVE
  To observe the effect of amiloride on the proteinuria of the 5/6 nephrectomy rats.
  
  
  METHODS
  To establish the 5/6 nephrectomy rats model and divide the experiment into 3 groups, sham operated group(Sham), 5/6 nephrectomy model group(NTX) and 5/6 nephrectomy with amiloride-treated group (NTX+amiloride, n=15). The concentration of protein and mRNA of uPAR and the change of podocytes motility were detected by coomassiebluestaining, immunofluorence method and real-time PCR.
  
  
  RESULTS
  At second week, compared with Control group, the 24 h urine protein of NTX group was significantly increased (47.50 ± 28.05 mg vs 14.28 ± 3.8 mg, P = 0.023). There was no statistical significance in 24-hour urine protein between NTX+amiloride group and NTX group (51.56 ± 21.03 mg vs 47.50 ± 28.05 mg, P = 0.748). The same situation was also observed at the time point of 12 week, comparing with NTX group, 24-hour urine protein decreased in Sham group (188.31 ± 29.82 mg vs 21.32 ± 8.59 mg, P = 0.000) and NTX+amiloride group (188.31 ± 29.82 mg vs 121.37 ± 31.14 mg, P=0.000), with statistical significance when comparing with Sham group, the expression of uPAR mRNA in NTX group was significantly increased (9.74 ± 1.44 vs 1.01 ± 0.13, P = 0.000). In contrast, the expression of uPAR mRNA in NTX rats treated with amiloride was significantly lower than in NTX group (9.74 ± 1.44 vs 5.01 ± 1.36, P = 0.000).
  
  
  CONCLUSIONS
  Amiloride can reduce the proteinuria of the 5/6 nephrectomy rats model of transient proteinuria by inhibiting the induction of uPAR expression.
authors.affiliation: Southern Medical University, Guangzhou 510515, China; Department of Nephrology, Guangdong General Hospital, Guangzhou 510080, China; Guangdong Academy of Medical Sciences, Guangzhou 510080, China. E-mail: hcs_gz@139.com.
authors.forename: Chaosheng
authors.initials: C
authors.lastname: He
authors.forename: Bin
authors.initials: B
authors.lastname: Zhang
authors.forename: Shaoting
authors.initials: S
authors.lastname: Xie
authors.forename: Yun
authors.initials: Y
authors.lastname: Yang
authors.forename: Juan
authors.initials: J
authors.lastname: Ma
authors.forename: Wei
authors.initials: W
authors.lastname: Shi
mesh_terms: D000584:Amiloride / Q000494:pharmacology*; D000818:Animals; D002465:Cell Movement; D004195:Disease Models, Animal; D009392:Nephrectomy; D050199:Podocytes / Q000187:drug effects* / Q000378:metabolism; D011507:Proteinuria / Q000188:drug therapy*; D051381:Rats; D060888:Real-Time Polymerase Chain Reaction; D055293:Receptors, Urokinase Plasminogen Activator / Q000378:metabolism*
chemical_list: D055293:Receptors, Urokinase Plasminogen Activator; D000584:Amiloride