title: Fabrication of new single cell chip to monitor intracellular and extracellular redox state based on spectroelectrochemical method.
abstract: Probing the local environment of target cells has been considered a challenging task due to the complexity of living cells. Here, we developed new single cell-based chip to investigate the intracellular and extracellular redox state of PC12 cells using spectroelectrochemical tool that combined surface-enhanced Raman scattering (SERS) and linear sweep voltammetry (LSV) techniques. PC12 cells immobilized on gold nanodots/ITO surface were subjected to LSV and their intracellular biochemical changes were successfully monitored by SERS simultaneously. Moreover, paired gold microelectrodes with micrometer-sized gap containing hexagonal array of gold nanodots were fabricated to detect electrochemical activity and changes in the redox environment of single PC12 cell based on SERS-LSV tool. This showed very effective detecting method. The used technology included the utilization of gold nanodots array inside micro-gap to enhance the Raman signals and the electrochemical activity of single cell. This could be used as an effective research tool to analyze cellular processes.
authors.affiliation: Interdisciplinary Program of Integrated Biotechnology, Sogang University, 35 Baekbeom-ro, Mapo-Gu, Seoul 121-742, Republic of Korea; Chemistry Department, Faculty of Science, Assiut University, Assiut 71516, Egypt.
authors.forename: Waleed Ahmed
authors.initials: WA
authors.lastname: El-Said
authors.affiliation: Department of Chemical & Biomolecular Engineering, Sogang University, 35 Baekbeom-ro, Mapo-gu, Seoul 121-742, Republic of Korea.
authors.forename: Tae-Hyung
authors.initials: TH
authors.lastname: Kim
authors.affiliation: Department of Chemical & Biomolecular Engineering, Sogang University, 35 Baekbeom-ro, Mapo-gu, Seoul 121-742, Republic of Korea.
authors.forename: Yong-Ho
authors.initials: YH
authors.lastname: Chung
authors.affiliation: Interdisciplinary Program of Integrated Biotechnology, Sogang University, 35 Baekbeom-ro, Mapo-Gu, Seoul 121-742, Republic of Korea; Department of Chemical & Biomolecular Engineering, Sogang University, 35 Baekbeom-ro, Mapo-gu, Seoul 121-742, Republic of Korea. Electronic address: jwchoi@sogang.ac.kr.
authors.forename: Jeong-Woo
authors.initials: JW
authors.lastname: Choi
mesh_terms: D000818:Animals; D003199:Computer Systems; D004298:Dopamine / Q000378:metabolism; D055664:Electrochemical Techniques / Q000379:methods*; D005110:Extracellular Space / Q000378:metabolism*; D042541:Intracellular Space / Q000378:metabolism*; D008839:Microelectrodes; D046210:Microfluidic Analytical Techniques; D055616:Microtechnology / Q000379:methods*; D018377:Neurotransmitter Agents / Q000378:metabolism; D010084:Oxidation-Reduction; D016716:PC12 Cells; D051381:Rats; D017971:Tin Compounds / Q000737:chemistry
chemical_list: D018377:Neurotransmitter Agents; D017971:Tin Compounds; C109984:indium tin oxide; D004298:Dopamine
keywords: Gold microelectrodes; Gold nanodots array; Intracellular and extracellular redox state; Single neural cell-chip; Spectroelectrochemical