title: Engineering a freestanding biomimetic cardiac patch using biodegradable poly(lactic-co-glycolic acid) (PLGA) and human embryonic stem cell-derived ventricular cardiomyocytes (hESC-VCMs).
abstract: Microgrooved thin PLGA film (≈30 μm) is successfully fabricated on a Teflon mold, which could be readily peeled off and is used for the construction of a biomimetic cardiac patch. The contraction of it is studied with optical mapping on transmembrane action potential. Our results suggest that steady-state contraction could be easily established on it under regular electrical stimuli. Besides, the biomimetic cardiac patch recapitulates the anisotropic electrophysiological feature of native cardiac tissue and is much more refractory to premature stimuli than the random one constructed with non-grooved PLGA film, as proved by the reduced incidence of arrhythmia. Considering the good biocompatibility of PLGA as demonstrated in our study and the biodegradability of it, our biomimetic cardiac patch may find applications in the treatment of myocardial infarction. Moreover, the Teflon mold could be applied in the fabrication of various scaffolds with fine features for other tissues.
authors.affiliation: Division of Biomedical Engineering, The Hong Kong University of Science and Technology, Hong Kong, China.
authors.forename: Yin
authors.initials: Y
authors.lastname: Chen
authors.forename: Junping
authors.initials: J
authors.lastname: Wang
authors.forename: Bo
authors.initials: B
authors.lastname: Shen
authors.forename: Camie W Y
authors.initials: CW
authors.lastname: Chan
authors.forename: Chaoyi
authors.initials: C
authors.lastname: Wang
authors.forename: Yihua
authors.initials: Y
authors.lastname: Zhao
authors.forename: Ho N
authors.initials: HN
authors.lastname: Chan
authors.forename: Qian
authors.initials: Q
authors.lastname: Tian
authors.forename: Yangfan
authors.initials: Y
authors.lastname: Chen
authors.forename: Chunlei
authors.initials: C
authors.lastname: Yao
authors.forename: I-Ming
authors.initials: IM
authors.lastname: Hsing
authors.forename: Ronald A
authors.initials: RA
authors.lastname: Li
authors.forename: Hongkai
authors.initials: H
authors.lastname: Wu
mesh_terms: D000200:Action Potentials; D001145:Arrhythmias, Cardiac / Q000628:therapy; D032701:Biomimetics / Q000379:methods*; D004558:Electric Stimulation; D006352:Heart Ventricles / Q000166:cytology*; D000066449:Human Embryonic Stem Cells / Q000166:cytology*; D006801:Humans; D019344:Lactic Acid / Q000737:chemistry*; D018613:Microscopy, Confocal; D009206:Myocardium / Q000473:pathology; D032383:Myocytes, Cardiac / Q000166:cytology*; D011100:Polyglycolic Acid / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D033581:Stem Cell Transplantation; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid
keywords: biodegradable; biomimetic cardiac patch; freestanding; human embryonic stem cell; poly(lactic-co-glycolic acid)