title: [Applications of MALDI-TOF technology in clinical microbiology].
abstract: Until now, the identification of micro-organisms has been based on the cultural and biochemical characteristics of bacterial and fungal species. Recently, Mass Spectrometry type Matrix-Assisted Laser Desorption Ionization-Time of Flight (MALDI-TOF MS) was developed in clinical microbiology laboratories. This new technology allows identification of micro-organisms directly from colonies of bacteria and fungi within few minutes. In addition, it can be used to identify germs directly from positive blood culture bottles or directly from urine samples. Other ways are being explored to expand the use of MALDI-TOF in clinical microbiology laboratories. Indeed, some studies propose to detect bacterial antibiotic resistance while others compare strains within species for faster strain typing. The main objective of this review is to update data from the recent literature for different applications of MALDI-TOF technique in microbiological diagnostic routine.
authors.affiliation: Laboratoire de microbiologie, hôpital Necker-Enfants-Malades, Assistance publique-Hôpitaux de Paris, 149, rue de Sèvres, 75015 Paris, France.
authors.forename: S
authors.initials: S
authors.lastname: Suarez
authors.affiliation: Laboratoire de microbiologie, hôpital Necker-Enfants-Malades, Assistance publique-Hôpitaux de Paris, 149, rue de Sèvres, 75015 Paris, France.
authors.forename: X
authors.initials: X
authors.lastname: Nassif
authors.affiliation: Laboratoire de microbiologie, hôpital Necker-Enfants-Malades, Assistance publique-Hôpitaux de Paris, 149, rue de Sèvres, 75015 Paris, France. Electronic address: agnes.ferroni@nck.aphp.fr.
authors.forename: A
authors.initials: A
authors.lastname: Ferroni
mesh_terms: D000818:Animals; D001419:Bacteria / Q000737:chemistry / Q000145:classification; D019411:Clinical Laboratory Techniques / Q000379:methods* / Q000592:standards; D003955:Diagnostic Tests, Routine / Q000379:methods / Q000592:standards; D005658:Fungi / Q000737:chemistry / Q000145:classification; D006801:Humans; D007239:Infections / Q000134:cerebrospinal fluid / Q000382:microbiology / Q000652:urine; D008826:Microbial Sensitivity Tests; D008828:Microbiological Techniques / Q000379:methods* / Q000592:standards; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods* / Q000592:standards
keywords: Identification microbienne; Mass spectrometry; Matrix-Assisted Laser Desorption Ionization-Time Of Flight (MALDI-TOF); Matrix-Assisted Laser Desorption Ionization-Time Of Flight (MALDI-TOF); Microbial identification; Microbiologie; Microbiology; Spectrométrie de masse