title: miR-224 overexpression is a strong and independent prognosticator of short-term relapse and poor overall survival in colorectal adenocarcinoma.
abstract: Colorectal adenocarcinoma constitutes the most frequent form of colorectal cancer and a serious cause of cancer-related deaths. The expression of multiple miRNAs, including miR-224, is deregulated in colorectal adenocarcinoma. The aim of this study was the investigation of the prognostic value of miR-224 in colorectal adenocarcinoma. For this purpose, total RNA was isolated from 115 colorectal adenocarcinomas and 66 adjacent non-cancer mucosae. Total RNA (2 µg) was polyadenylated and reverse transcribed. A quantitative PCR method based on SYBR-Green chemistry was developed and applied for the quantification of miR-224 levels, followed by extensive biostatistical analysis. miR-224 levels in malignant colorectal adenocarcinomas ranged between 1.81 and 187.75 RQU (miR-224 copies/1,000 SNORD48 copies) with a median of 34.27, and were significantly elevated, compared to miR-224 levels in adjacent non-cancer mucosae (p<0.001). Enhanced miR-224 expression constitutes a rather strong prognosticator in colorectal adenocarcinoma, predicting short-term relapse and poor overall survival in these patients (p=0.012 and p=0.005, respectively), independent of established clinicopathological parameters. In conclusion, miR-224 is significantly upregulated in malignant colorectal tumors compared to adjacent non-cancer mucosae, and its enhanced expression constitutes an independent predictor of short-term relapse and poor overall survival in colorectal adenocarcinoma patients.
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Athens, Panepistimiopolis, Athens, Greece.
authors.forename: Panagiotis G
authors.initials: PG
authors.lastname: Adamopoulos
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Athens, Panepistimiopolis, Athens, Greece.
authors.forename: Christos K
authors.initials: CK
authors.lastname: Kontos
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Athens, Panepistimiopolis, Athens, Greece.
authors.forename: Stamatia-Maria
authors.initials: SM
authors.lastname: Rapti
authors.affiliation: Fourth Surgery Department, University of Athens, University General Hospital 'Attikon', Athens, Greece.
authors.forename: Iordanis N
authors.initials: IN
authors.lastname: Papadopoulos
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Athens, Panepistimiopolis, Athens, Greece.
authors.forename: Andreas
authors.initials: A
authors.lastname: Scorilas
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics* / Q000473:pathology; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000473:pathology; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D053208:Kaplan-Meier Estimate; D008207:Lymphatic Metastasis; D008297:Male; D035683:MicroRNAs / Q000096:biosynthesis* / Q000235:genetics; D008875:Middle Aged; D009364:Neoplasm Recurrence, Local / Q000235:genetics* / Q000473:pathology; D011379:Prognosis; D012008:Recurrence
chemical_list: C546140:MIRN224 microRNA, human; D035683:MicroRNAs