title: Mouse dead end 1-β interacts with c-Jun and stimulates activator protein 1 transactivation.
abstract: Dead end 1 (DND1), important for maintaining the viability of primordial germ cells, is the first protein containing an RNA recognition motif that has been directly implicated as a heritable cause of spontaneous tumorigenesis. In the present study, c-Jun was identified through yeast two-hybrid screening of a 10.5-day old mouse embryo cDNA library as one of the proteins which interact with DND1-β. The interaction between DND1-β and c-Jun was demonstrated to occur by glutathione S‑transferase pull‑down and co-immunoprecipitation. Using confocal microscopy, DND1-β was found to be specifically expressed in GC-1 spermatogonia cells, mainly in the nuclei. When transfected into GC-1 cells, DND1-β and c-Jun were demonstrated to be co-localized principally in the nuclei. Furthermore, in a dual luciferase reporter assay, the transcriptional activity of activator protein 1 was demonstrated to be significantly increased by co-transfection with DND1-β and c-Jun plasmids in GC-1 cells. The identification and confirmation of an additional protein interacting with DND1-β facilitates the investigation of the functions and molecular mechanisms of DND1.
authors.affiliation: Department of Internal Medicine, College of Medicine, Hunan Normal University, Changsha, Hunan 410013, P.R. China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Internal Medicine, College of Medicine, Hunan Normal University, Changsha, Hunan 410013, P.R. China.
authors.forename: Yan-Lin
authors.initials: YL
authors.lastname: Su
authors.affiliation: Department of Gynecologic Oncology, Cancer Hospital of Xiangya School of Medicine of Central South University, Changsha, Hunan 410013, P.R. China.
authors.forename: Le-Sai
authors.initials: LS
authors.lastname: Li
authors.affiliation: Department of Internal Medicine, College of Medicine, Hunan Normal University, Changsha, Hunan 410013, P.R. China.
authors.forename: Zhi
authors.initials: Z
authors.lastname: Yang
authors.affiliation: Department of Internal Medicine, College of Medicine, Hunan Normal University, Changsha, Hunan 410013, P.R. China.
authors.forename: Si
authors.initials: S
authors.lastname: Chen
authors.affiliation: Department of Internal Medicine, College of Medicine, Hunan Normal University, Changsha, Hunan 410013, P.R. China.
authors.forename: Jie
authors.initials: J
authors.lastname: Xiong
authors.affiliation: Department of Internal Medicine, College of Medicine, Hunan Normal University, Changsha, Hunan 410013, P.R. China.
authors.forename: Xiao-Hua
authors.initials: XH
authors.lastname: Fu
authors.affiliation: Department of Internal Medicine, College of Medicine, Hunan Normal University, Changsha, Hunan 410013, P.R. China.
authors.forename: Xiao-Ning
authors.initials: XN
authors.lastname: Peng
mesh_terms: D000818:Animals; D002460:Cell Line; D005786:Gene Expression Regulation; D051379:Mice; D009363:Neoplasm Proteins / Q000378:metabolism*; D011485:Protein Binding; D025941:Protein Interaction Mapping; D016755:Proto-Oncogene Proteins c-jun / Q000378:metabolism*; D018808:Transcription Factor AP-1 / Q000378:metabolism*; D015533:Transcriptional Activation; D020798:Two-Hybrid System Techniques
chemical_list: C500862:Dnd1 protein, mouse; D009363:Neoplasm Proteins; D016755:Proto-Oncogene Proteins c-jun; D018808:Transcription Factor AP-1