title: N-Glycosylation and plant cell growth.
abstract: N-linked glycosylation is one of the most prevalent cotranslational protein modifications in plants. It is initiated by a conserved process in the endoplasmic reticulum and subsequently involves a series of different N-glycan maturation steps that take place in the ER and Golgi apparatus. Despite our vast knowledge on the different processing steps we still understand very little about the role of characteristic glycoforms present on individual plant glycoproteins. Here, we describe convenient tools that allow the fast and reliable characterization of N-glycosylation on plant glycoproteins. The presented protocols can be adopted to other plant species and to the characterization of N-glycans from different glycoproteins.
authors.affiliation: Department of Applied Genetics and Cell Biology, University of Natural Resources and Life Sciences, Vienna, Austria.
authors.forename: Christiane
authors.initials: C
authors.lastname: Veit
authors.forename: Ulrike
authors.initials: U
authors.lastname: Vavra
authors.forename: Richard
authors.initials: R
authors.lastname: Strasser
mesh_terms: D017360:Arabidopsis / Q000166:cytology* / Q000254:growth & development* / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000378:metabolism; D004721:Endoplasmic Reticulum / Q000378:metabolism; D006023:Glycoproteins / Q000378:metabolism*; D006031:Glycosylation; D006056:Golgi Apparatus / Q000378:metabolism
chemical_list: D029681:Arabidopsis Proteins; D006023:Glycoproteins