title: Effect of different periods of preconditioning with saliva on Candida albicans adhesion to a denture base resin by crystal violet staining and XTT assay.
abstract: OBJECTIVE
  The role of saliva on Candida adhesion to biomaterials has not been clearly defined. The present study investigates whether different periods of preconditioning with saliva would influence the adhesion of Candida albicans to a denture base resin.
  
  
  METHODS
  Ninety samples of acrylic resin with smooth surfaces were made and then divided into five groups: one control without saliva, and four experimental groups conditioned in saliva for periods of 30 min, 1, 3, or 12 h. Candida adhesion was evaluated by crystal violet staining and 2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-5-([phenylamino] carbonyl)-2H-tetrazolium-hydroxide assay.
  
  
  RESULTS
  The one-way analysis of variance revealed that there were no significant differences among the mean number of adherent cells or among the mean absorbance for all groups. No significant correlation was found between the two methods used for assessing Candida albicans adhesion.
  
  
  CONCLUSIONS
  The different periods of preconditioning with saliva had no significant influence on the adhesion of Candida albicans to the denture base acrylic resin.
authors.affiliation: Department of Dental Materials and Prosthodontics, UNESP--São Paulo State University, Araraquara, Brazil.
authors.forename: Camila Andrade
authors.initials: CA
authors.lastname: Zamperini
authors.forename: Patrícia Cristiane dos Santos
authors.initials: PC
authors.lastname: Schiavinato
authors.forename: Ana Lucia
authors.initials: AL
authors.lastname: Machado
authors.forename: Eunice Teresinha
authors.initials: ET
authors.lastname: Giampaolo
authors.forename: Ana Claudia
authors.initials: AC
authors.lastname: Pavarina
authors.forename: Carlos Eduardo
authors.initials: CE
authors.lastname: Vergani
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry*; D000268:Adhesiveness; D000328:Adult; D002176:Candida albicans / Q000502:physiology*; D015169:Colony Count, Microbial; D004396:Coloring Agents; D003764:Dental Materials / Q000737:chemistry*; D044622:Dental Pellicle / Q000502:physiology*; D003819:Denture Bases / Q000382:microbiology*; D005840:Gentian Violet; D006801:Humans; D007202:Indicators and Reagents; D008827:Microbiological Phenomena; D012463:Saliva / Q000502:physiology*; D013499:Surface Properties; D013778:Tetrazolium Salts; D013997:Time Factors
chemical_list: D000180:Acrylic Resins; D004396:Coloring Agents; D003764:Dental Materials; D007202:Indicators and Reagents; D013778:Tetrazolium Salts; C059087:2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-5-((phenylamino)carbonyl)-2H-tetrazolium hydroxide; D005840:Gentian Violet
keywords: Candida albicans; acrylic resin; adhesion; biofilm; saliva