title: Efficient production of (R,R)-2,3-butanediol from cellulosic hydrolysate using Paenibacillus polymyxa ICGEB2008.
abstract: We report here the production of pure (R,R)-2,3-butanediol (2,3-BDO) isomer by the non-pathogenic Paenibacillus polymyxa ICGEB2008 using lignocellulosic hydrolysate as substrate. Experimental design based on Plackett-Burman resulted in identification of Mn and K as most crucial salt elements along with the yeast extract for 2,3-BDO production. Further experiments using Box-Behnken design indicated that both KCl and yeast extract together had major impact on 2,3-BDO production. Optimized medium resulted in 2,3-BDO production with 2.3-fold higher maximum volumetric productivity (2.01 g/L/h) and similar yield (0.33 g/g sugar) as compared to rich yeast extract-peptone-dextrose medium in the bioreactor studies. Considering that the balance substrate was channeled towards ethanol, carbon recovery was close to theoretical yield between the two solvents, i.e., 2,3-BDO and ethanol. Biomass hydrolysate and corn-steep liquor was used further to produce 2,3-BDO without impacting its yield. In addition, 2,3-BDO was also produced via simultaneous saccharification and fermentation, signifying robustness of the strain.
authors.affiliation: Synthetic Biology and Biofuels Group, International Centre for Genetic Engineering and Biotechnology (ICGEB), 10504, Aruna Asaf Ali Marg, New Delhi, 110067, India.
authors.forename: Nidhi
authors.initials: N
authors.lastname: Adlakha
authors.forename: Syed Shams
authors.initials: SS
authors.lastname: Yazdani
mesh_terms: D018533:Biomass; D019149:Bioreactors / Q000382:microbiology; D002072:Butylene Glycols / Q000378:metabolism*; D002482:Cellulose / Q000737:chemistry; D003470:Culture Media / Q000737:chemistry; D000431:Ethanol / Q000378:metabolism; D005285:Fermentation; D005947:Glucose / Q000378:metabolism; D056507:Paenibacillus / Q000378:metabolism*
chemical_list: D002072:Butylene Glycols; D003470:Culture Media; D000431:Ethanol; C026978:2,3-butylene glycol; D002482:Cellulose; D005947:Glucose