title: Nitric oxide regulates synaptic transmission between spiny projection neurons.
abstract: Recurrent axon collaterals are a major means of communication between spiny projection neurons (SPNs) in the striatum and profoundly affect the function of the basal ganglia. However, little is known about the molecular and cellular mechanisms that underlie this communication. We show that intrastriatal nitric oxide (NO) signaling elevates the expression of the vesicular GABA transporter (VGAT) within recurrent collaterals of SPNs. Down-regulation of striatal NO signaling resulted in an attenuation of GABAergic signaling in SPN local collaterals, down-regulation of VGAT expression in local processes of SPNs, and impaired motor behavior. PKG1 and cAMP response element-binding protein are involved in the signal transduction that transcriptionally regulates VGAT by NO. These data suggest that transcriptional control of the vesicular GABA transporter by NO regulates GABA transmission and action selection.
authors.affiliation: Laboratory of Molecular and Cellular Neuroscience, The Rockefeller University, New York, NY 10065; greengard@rockefeller.edu ysagi@rockefeller.edu.
authors.forename: Yotam
authors.initials: Y
authors.lastname: Sagi
authors.affiliation: Laboratory of Molecular and Cellular Neuroscience, The Rockefeller University, New York, NY 10065;
authors.forename: Myriam
authors.initials: M
authors.lastname: Heiman
authors.affiliation: Department of Physiology, Northwestern University Feinberg School of Medicine, Chicago, IL 60611;
authors.forename: Jayms D
authors.initials: JD
authors.lastname: Peterson
authors.affiliation: Laboratory of Molecular Neurosurgery, Weill Medical College of Cornell University, New York, NY 10065; and.
authors.forename: Sergei
authors.initials: S
authors.lastname: Musatov
authors.forename: Mariangela
authors.initials: M
authors.lastname: Scarduzio
authors.forename: Stephen M
authors.initials: SM
authors.lastname: Logan
authors.affiliation: Laboratory of Molecular Neurosurgery, Weill Medical College of Cornell University, New York, NY 10065; and.
authors.forename: Michael G
authors.initials: MG
authors.lastname: Kaplitt
authors.affiliation: Department of Physiology, Northwestern University Feinberg School of Medicine, Chicago, IL 60611;
authors.forename: Dalton J
authors.initials: DJ
authors.lastname: Surmeier
authors.affiliation: Laboratory of Molecular Biology and Howard Hughes Medical Institute, The Rockefeller University, New York, NY 10065.
authors.forename: Nathaniel
authors.initials: N
authors.lastname: Heintz
authors.affiliation: Laboratory of Molecular and Cellular Neuroscience, The Rockefeller University, New York, NY 10065; greengard@rockefeller.edu ysagi@rockefeller.edu.
authors.forename: Paul
authors.initials: P
authors.lastname: Greengard
mesh_terms: D000818:Animals; D001369:Axons / Q000378:metabolism; D001479:Basal Ganglia / Q000378:metabolism*; D000242:Cyclic AMP / Q000378:metabolism; D004298:Dopamine / Q000378:metabolism; D004594:Electrophysiology; D025461:Feedback, Physiological; D005260:Female; D049452:Green Fluorescent Proteins / Q000378:metabolism; D006162:Guanylate Cyclase / Q000737:chemistry*; D007980:Levodopa / Q000737:chemistry; D008297:Male; D051379:Mice; D009473:Neuronal Plasticity; D009474:Neurons / Q000378:metabolism*; D009569:Nitric Oxide / Q000737:chemistry*; D016627:Oxidopamine / Q000737:chemistry; D015398:Signal Transduction; D009435:Synaptic Transmission / Q000502:physiology*; D050495:Vesicular Inhibitory Amino Acid Transport Proteins / Q000378:metabolism; D005680:gamma-Aminobutyric Acid / Q000737:chemistry*
chemical_list: D050495:Vesicular Inhibitory Amino Acid Transport Proteins; C492244:Viaat protein, mouse; D049452:Green Fluorescent Proteins; D009569:Nitric Oxide; D007980:Levodopa; D005680:gamma-Aminobutyric Acid; D016627:Oxidopamine; D000242:Cyclic AMP; D006162:Guanylate Cyclase; D004298:Dopamine
keywords: BacTRAP; axon collaterals; guanylyl cyclase; spiny projecting neurons; vesicular GABA transporter