title: Generating and measuring photochemical changes inside the brain using optical fibers: exploring stroke.
abstract: We report here on the development of a method for inducing a stroke in a specific location within a mouse brain through the use of an optical fiber. By capturing the emitted fluorescence signal generated using the same fiber it is possible to monitor photochemical changes within the brain in real-time, and directly measure the concentration of the stroke-inducing dye, Rose Bengal, at the infarct site. This technique reduces the requirement for post-operative histology to determine if a stroke has successfully been induced within the animal, and therefore opens up the opportunity to explore the recovery of the brain after the stroke event.
authors.affiliation: ARC Centre of Excellence for Nanoscale BioPhotonics, Institute for Photonics and Advanced Sensing, School of Chemistry and Physics, The University of Adelaide, Adelaide, SA 5005, Australia.
authors.forename: Georgios
authors.initials: G
authors.lastname: Tsiminis
authors.affiliation: Stroke Research Programme, School of Medicine, The University of Adelaide, Adelaide, SA 5005, Australia.
authors.forename: Thomas S
authors.initials: TS
authors.lastname: Klarić
authors.affiliation: ARC Centre of Excellence for Nanoscale BioPhotonics, Institute for Photonics and Advanced Sensing, School of Chemistry and Physics, The University of Adelaide, Adelaide, SA 5005, Australia.
authors.forename: Erik P
authors.initials: EP
authors.lastname: Schartner
authors.affiliation: ARC Centre of Excellence for Nanoscale BioPhotonics, Institute for Photonics and Advanced Sensing, School of Chemistry and Physics, The University of Adelaide, Adelaide, SA 5005, Australia.
authors.forename: Stephen C
authors.initials: SC
authors.lastname: Warren-Smith
authors.affiliation: Mind & Brain Theme, South Australian Health & Medical Research Institute, Adelaide, SA 5000, Australia.
authors.forename: Martin D
authors.initials: MD
authors.lastname: Lewis
authors.affiliation: Stroke Research Programme, School of Medicine, The University of Adelaide, Adelaide, SA 5005, Australia.
authors.forename: Simon A
authors.initials: SA
authors.lastname: Koblar
authors.affiliation: ARC Centre of Excellence for Nanoscale BioPhotonics, Institute for Photonics and Advanced Sensing, School of Chemistry and Physics, The University of Adelaide, Adelaide, SA 5005, Australia.
authors.forename: Tanya M
authors.initials: TM
authors.lastname: Monro
keywords: (060.2370) Fiber optics sensors; (170.0170) Medical optics and biotechnology; (170.3890) Medical optics instrumentation; (170.6280) Spectroscopy, fluorescence and luminescence