title: Lipid raft-associated β-adducin is required for PSGL-1-mediated neutrophil rolling on P-selectin.
abstract: Lipid rafts, a liquid-ordered plasma membrane microdomain, are related to cell-surface receptor function. PSGL-1, a major surface receptor protein for leukocyte, also acts as a signaling receptor in leukocyte rolling. To investigate the role of lipid raft in PSGL-1 signaling in human neutrophils, we quantitatively analyzed lipid raft proteome of human promyelocytic leukemia cell line HL-60 cells and identified a lipid raft-associated protein β-adducin. PSGL-1 ligation induced dissociation of the raft-associated protein β-adducin from lipid rafts and actin, as well as phosphorylation of β-adducin, indicating a transient uncoupling of lipid rafts from the actin cytoskeleton. Knockdown of β-adducin greatly attenuated HL-60 cells rolling on P-selectin. We also showed that Src kinase is crucial for PSGL-1 ligation-induced β-adducin phosphorylation and relocation. Taken together, these results show that β-adducin is a pivotal lipid raft-associated protein in PSGL-1-mediated neutrophil rolling on P-selectin.
authors.affiliation: *Institute of Genetics and Cytology, Northeast Normal University, Changchun, China; Department of Bioscience, Changchun Normal University, Changchun, China; and Department of Pathophysiology, Southern Medical University, Guangzhou, China.
authors.forename: Tingshuang
authors.initials: T
authors.lastname: Xu
authors.affiliation: *Institute of Genetics and Cytology, Northeast Normal University, Changchun, China; Department of Bioscience, Changchun Normal University, Changchun, China; and Department of Pathophysiology, Southern Medical University, Guangzhou, China.
authors.forename: Wenai
authors.initials: W
authors.lastname: Liu
authors.affiliation: *Institute of Genetics and Cytology, Northeast Normal University, Changchun, China; Department of Bioscience, Changchun Normal University, Changchun, China; and Department of Pathophysiology, Southern Medical University, Guangzhou, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Yang
authors.affiliation: *Institute of Genetics and Cytology, Northeast Normal University, Changchun, China; Department of Bioscience, Changchun Normal University, Changchun, China; and Department of Pathophysiology, Southern Medical University, Guangzhou, China.
authors.forename: Xueqing
authors.initials: X
authors.lastname: Ba
authors.affiliation: *Institute of Genetics and Cytology, Northeast Normal University, Changchun, China; Department of Bioscience, Changchun Normal University, Changchun, China; and Department of Pathophysiology, Southern Medical University, Guangzhou, China zengx779@nenu.edu.cn wangxiaoguang58@sina.com.
authors.forename: Xiaoguang
authors.initials: X
authors.lastname: Wang
authors.affiliation: *Institute of Genetics and Cytology, Northeast Normal University, Changchun, China; Department of Bioscience, Changchun Normal University, Changchun, China; and Department of Pathophysiology, Southern Medical University, Guangzhou, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Jiang
authors.affiliation: *Institute of Genetics and Cytology, Northeast Normal University, Changchun, China; Department of Bioscience, Changchun Normal University, Changchun, China; and Department of Pathophysiology, Southern Medical University, Guangzhou, China zengx779@nenu.edu.cn wangxiaoguang58@sina.com.
authors.forename: Xianlu
authors.initials: X
authors.lastname: Zeng
mesh_terms: D000328:Adult; D003598:Cytoskeletal Proteins / Q000276:immunology*; D018922:HL-60 Cells; D006801:Humans; D036904:Leukocyte Rolling / Q000502:physiology*; D008562:Membrane Glycoproteins / Q000276:immunology*; D021962:Membrane Microdomains / Q000276:immunology*; D009504:Neutrophils / Q000166:cytology / Q000276:immunology*; D019007:P-Selectin / Q000276:immunology*; D010766:Phosphorylation; D021381:Protein Transport / Q000276:immunology; D000099291:Adducins
chemical_list: D003598:Cytoskeletal Proteins; D008562:Membrane Glycoproteins; D019007:P-Selectin; C501062:ADD2 protein, human; D000099291:Adducins; C084882:P-selectin ligand protein; C546937:SELP protein, human
keywords: LC-MS/MS; Src kinase; phosphorylation; polarization