title: Dynamic properties of sensory stimulation evoked responses in mouse cerebellar granule cell layer and molecular layer.
abstract: Sensory information coming from climbing fiber and mossy fiber-granule cell pathways, generates motor-related outputs according to internal rules of integration and computation in the cerebellar cortex. However, the dynamic properties of sensory information processing in mouse cerebellar cortex are less understood. Here, we studied the dynamic properties of sensory stimulation-evoked responses in the cerebellar granule cell layer (GCL) and molecular layer (ML) by electrophysiological recordings method. Our data showed that air-puff stimulation (5-10 ms in duration) of the ipsilateral whisker pad evoked single-peak responses in the GCL and ML; whereas a duration of stimulation ≥30 ms in GCL and ≥60 ms in ML, evoked double-peak responses that corresponded with stimulation-on and -off responses via mossy fiber pathway. The highest frequency of stimulation train for evoking GCL responses was 33 Hz. In contrast, the highest frequency of stimulation train for evoking ML responses was 4 Hz. These results indicate that the cerebellar granule cells transfer the high-fidelity sensory information from mossy fibers, which is cut-off by molecular layer interneurons (MLIs). Our results suggest that the MLIs network acts as a low-pass filter during the processing of high-frequency sensory information.
authors.affiliation: Cellular Function Research Center, Yanbian University, Yanji City, Jilin Province, China; Department of Physiology and Pathophysiology, College of Medicine, Yanbian University, Yanji City, Jilin Province, China.
authors.forename: Yan-Hua
authors.initials: YH
authors.lastname: Bing
authors.affiliation: Cellular Function Research Center, Yanbian University, Yanji City, Jilin Province, China; Department of Physiology and Pathophysiology, College of Medicine, Yanbian University, Yanji City, Jilin Province, China; Department of Pain, Affiliated Hospital of Yanbian University, Yanji City, Jilin Province, China.
authors.forename: Guang-Jian
authors.initials: GJ
authors.lastname: Zhang
authors.affiliation: Cellular Function Research Center, Yanbian University, Yanji City, Jilin Province, China; Department of Physiology and Pathophysiology, College of Medicine, Yanbian University, Yanji City, Jilin Province, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Sun
authors.affiliation: Cellular Function Research Center, Yanbian University, Yanji City, Jilin Province, China; Department of Physiology and Pathophysiology, College of Medicine, Yanbian University, Yanji City, Jilin Province, China. Electronic address: cpchu@ybu.edu.cn.
authors.forename: Chun-Ping
authors.initials: CP
authors.lastname: Chu
authors.affiliation: Cellular Function Research Center, Yanbian University, Yanji City, Jilin Province, China; Department of Physiology and Pathophysiology, College of Medicine, Yanbian University, Yanji City, Jilin Province, China. Electronic address: dlqiu@ybu.edu.cn.
authors.forename: De-Lai
authors.initials: DL
authors.lastname: Qiu
mesh_terms: D000818:Animals; D002525:Cerebellar Cortex / Q000166:cytology / Q000502:physiology*; D005071:Evoked Potentials; D005260:Female; D007395:Interneurons / Q000502:physiology; D008297:Male; D008813:Mice, Inbred ICR; D009415:Nerve Net / Q000502:physiology; D009474:Neurons / Q000502:physiology*; D010812:Physical Stimulation; D014110:Touch; D014738:Vibrissae / Q000502:physiology*
keywords: Electrophysiological recording; Granule cell; Molecular layer interneuron; Mouse cerebellar cortex; Sensory stimulation