title: Sulfation of phenylephrine by the human cytosolic sulfotransferases.
abstract: Previous studies had demonstrated that sulfation constituted a major pathway for the metabolism of phenylephrine in vivo. The current study was designed to identify the major human SULT(s) responsible for the sulfation of phenylephrine. Of the twelve human SULTs analyzed, SULT1A3 displayed the strongest sulfating activity toward phenylephrine. The enzyme exhibited a pH optimum spanning 7 - 10.5. Kinetic analysis revealed that SULT1A3- mediated sulfation of phenylephrine occurred in the same order of magnitude compared with that previously reported for SULT1A3-mediated sulfation of dopamine. Moreover, sulfation of phenylephrine was shown to occur in HepG2 cells under metabolic setting. Collectively, these results provided useful information concerning the biochemical basis underlying the metabolism of phenylephrine in vivo as previously reported.
authors.forename: Akihiro
authors.initials: A
authors.lastname: Yamamoto
authors.forename: Jiwan
authors.initials: J
authors.lastname: Kim
authors.forename: Ming-Yih
authors.initials: MY
authors.lastname: Liu
authors.forename: Katsuhisa
authors.initials: K
authors.lastname: Kurogi
authors.forename: Yoichi
authors.initials: Y
authors.lastname: Sakakibara
authors.forename: Masahito
authors.initials: M
authors.lastname: Suiko
authors.affiliation: Department of Pharmacology, College of Pharmacy and Pharmaceutical Sciences, University of Toledo Health Science Campus, 3000 Arlington Avenue, Toledo, OH 43614 USA. ming.liu@utoledo.edu.
authors.forename: Ming-Cheh
authors.initials: MC
authors.lastname: Liu
mesh_terms: D058646:Adrenergic alpha-1 Receptor Agonists / Q000378:metabolism; D015239:Arylsulfotransferase / Q000378:metabolism*; D003600:Cytosol / Q000201:enzymology*; D056945:Hep G2 Cells; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D010656:Phenylephrine / Q000378:metabolism*; D013431:Sulfates / Q000378:metabolism; D015238:Sulfotransferases / Q000378:metabolism*
chemical_list: D058646:Adrenergic alpha-1 Receptor Agonists; D013431:Sulfates; D010656:Phenylephrine; D015238:Sulfotransferases; D015239:Arylsulfotransferase; C403170:monoamine-sulfating phenol sulfotransferase