title: Fibronectin stimulates migration and proliferation, but not chondrogenic differentiation of human subchondral progenitor cells.
abstract: OBJECTIVE
  To evaluate the impact of human plasma-derived fibronectin (FN) on human subchondral mesenchymal progenitor cells regarding cell migration, proliferation, and chondrogenic differentiation.
  
  
  METHODS
  Human subchondral mesenchymal progenitor cells were analyzed for their migration capacity upon treatment with human plasma-derived FN. Proliferation activity was evaluated by DNA content. For chondrogenesis, cells were cultured in high-density pellet cultures in the presence of FN, TGFβ3, and a combination thereof.
  
  
  RESULTS
  Treatment of progenitors with FN significantly increased the number of migrating cells and elevated proliferative activity. Histological staining indicated formation of an extracellular matrix with type II collagen. Gene expression analysis gave no evidence for chondrogenic differentiation mediated by FN, but revealed a significant induction of type II collagen expression.
  
  
  CONCLUSIONS
  FN has a potential to recruit human subchondral mesenchymal progenitor cells, possibly supporting proliferation and matrix assembly in cartilage repair procedures using bioactive implants after microfracture treatment.
authors.affiliation: TransTissue Technologies GmbH, Charitéplatz 1, 10117 Berlin, Germany.
authors.forename: Richard Horst
authors.initials: RH
authors.lastname: Kalkreuth
authors.forename: Jan Philipp
authors.initials: JP
authors.lastname: Krüger
authors.forename: Skadi
authors.initials: S
authors.lastname: Lau
authors.forename: Philipp
authors.initials: P
authors.lastname: Niemeyer
authors.forename: Michaela
authors.initials: M
authors.lastname: Endres
authors.forename: Peter Cornelius
authors.initials: PC
authors.lastname: Kreuz
authors.forename: Christian
authors.initials: C
authors.lastname: Kaps
mesh_terms: D000368:Aged; D002358:Cartilage, Articular / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects*; D002465:Cell Movement / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D002633:Chemotaxis; D019902:Chondrocytes / Q000166:cytology*; D020219:Chondrogenesis / Q000187:drug effects; D024043:Collagen Type II / Q000235:genetics / Q000378:metabolism; D005109:Extracellular Matrix / Q000187:drug effects / Q000378:metabolism; D005260:Female; D005353:Fibronectins / Q000494:pharmacology*; D006801:Humans; D007124:Immunoenzyme Techniques; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D016133:Polymerase Chain Reaction; D013234:Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism
chemical_list: D024043:Collagen Type II; D005353:Fibronectins
keywords: cartilage regeneration; chondrogenesis; fibronectin; microfracture; migration; proliferation