title: Biohydrogen production in an AnSBBR treating glycerin-based wastewater: effects of organic loading, influent concentration, and cycle time.
abstract: This study evaluated the influence of the applied volumetric organic load on biohydrogen production in an anaerobic sequencing batch biofilm reactor (AnSBBR) with 3.5 L of liquid medium and treating 1.5 L of glycerin-based wastewater per cycle at 30 °C. Six applied volumetric organic loads (AVOLCT) were generated from the combination of cycle periods (3 and 4 h) and influent concentrations (3000, 4000, and 5000 mg chemical oxygen demand (COD) L(-1)), with values ranging from 7565 to 16,216 mg COD L(-1) day(-1). No clear relationship was found between the applied volumetric organic load and the hydrogen production. However, the highest hydrogen molar production (MPr 67.5 mol H2 m(-3) day(-1)) was reached when the reactor was operated with a cycle period of 4 h and an influent concentration of 5000 mg COD L(-1) (AVOLCT 12,911 mg COD L(-1) day(-1)). This condition also reached the highest molar yield per applied load based on the organic matter (MYALC,m 21.1 mol H2 kg COD(-1)). In addition, the pretreatment of the sludge/inoculum was found to not influence the productivity/yield of the process, and the use of crude glycerol as a sole source of carbon exhibited a clear disadvantage for hydrogen production compared to pure glycerol. The AnSBBR used for the hydrogen production experiments operated with pure glycerol as a sole carbon source exhibited important practical potential.
authors.affiliation: Escola de Engenharia de São Carlos, Universidade de São Paulo (USP), Av. Trabalhador São-Carlense 400, São Carlos, SP, 13566-590, Brazil.
authors.forename: I S Moncayo
authors.initials: IS
authors.lastname: Bravo
authors.forename: G
authors.initials: G
authors.lastname: Lovato
authors.forename: J A D
authors.initials: JA
authors.lastname: Rodrigues
authors.forename: S M
authors.initials: SM
authors.lastname: Ratusznei
authors.forename: M
authors.initials: M
authors.lastname: Zaiat
mesh_terms: D000693:Anaerobiosis; D001419:Bacteria / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D061249:Batch Cell Culture Techniques / Q000379:methods; D018441:Biofilms / Q000187:drug effects* / Q000254:growth & development; D019149:Bioreactors; D005285:Fermentation / Q000187:drug effects; D005990:Glycerol / Q000378:metabolism / Q000494:pharmacology*; D006859:Hydrogen / Q000378:metabolism*; D012722:Sewage / Q000382:microbiology*
chemical_list: D012722:Sewage; D006859:Hydrogen; D005990:Glycerol