title: Gene expression of tendon markers in mesenchymal stromal cells derived from different sources.
abstract: BACKGROUND
  Multipotent mesenchymal stromal cells (MSC) can be recovered from a variety of tissues in the body. Yet, their functional properties were shown to vary depending on tissue origin. While MSC have emerged as a favoured cell type for tendon regenerative therapies, very little is known about the influence of the MSC source on their properties relevant to tendon regeneration.The aim of this study was to assess and compare the expression of tendon extracellular matrix proteins and tendon differentiation markers in MSC derived from different sources as well as in native tendon tissue. MSC isolated from equine bone marrow, adipose tissue, umbilical cord tissue, umbilical cord blood and tendon tissue were characterized and then subjected to mRNA analysis by real-time polymerase chain reaction.
  
  
  RESULTS
  MSC derived from adipose tissue displayed the highest expression of collagen 1A2, collagen 3A1 and decorin compared to MSC from all other sources and native tendon tissue (p < 0.01). Tenascin-C and scleraxis expressions were highest in MSC derived from cord blood compared to MSC derived from other sources, though both tenascin-C and scleraxis were expressed at significantly lower levels in all MSC compared to native tendon tissue (p < 0.01).
  
  
  CONCLUSIONS
  These findings demonstrate that the MSC source impacts the cell properties relevant to tendon regeneration. Adipose derived MSC might be superior regarding their potential to positively influence tendon matrix reorganization.
authors.affiliation: Translational Centre for Regenerative Medicine (TRM), University of Leipzig, Philipp-Rosenthal-Strasse 55, 04103 Leipzig, Germany. burk@rz.uni-leipzig.de.
authors.forename: Janina
authors.initials: J
authors.lastname: Burk
authors.forename: Claudia
authors.initials: C
authors.lastname: Gittel
authors.forename: Sandra
authors.initials: S
authors.lastname: Heller
authors.forename: Bastian
authors.initials: B
authors.lastname: Pfeiffer
authors.forename: Felicitas
authors.initials: F
authors.lastname: Paebst
authors.forename: Annette B
authors.initials: AB
authors.lastname: Ahrberg
authors.forename: Walter
authors.initials: W
authors.lastname: Brehm
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D001854:Bone Marrow Cells / Q000378:metabolism / Q000502:physiology; D002454:Cell Differentiation; D019070:Cell Lineage; D002469:Cell Separation / Q000379:methods; D002478:Cells, Cultured; D016326:Extracellular Matrix Proteins / Q000235:genetics / Q000378:metabolism; D005312:Fetal Blood / Q000166:cytology; D005786:Gene Expression Regulation; D006736:Horses; D059630:Mesenchymal Stem Cells / Q000378:metabolism / Q000502:physiology*; D012333:RNA, Messenger / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D013710:Tendons / Q000166:cytology*; D014470:Umbilical Cord / Q000166:cytology
chemical_list: D015415:Biomarkers; D016326:Extracellular Matrix Proteins; D012333:RNA, Messenger