title: BmPLA2 containing conserved domain WD40 affects the metabolic functions of fat body tissue in silkworm, Bombyx mori.
abstract: PLA2 enzyme hydrolyzes arachidonic acid, and other polyunsaturated fatty acids, from the sn-2 position to release free arachidonic acid and a lysophospholipid. Previous studies reported that the PLA2 in invertebrate organisms participates in lipid signaling molecules like arachidonic acid release in immune-associated tissues like hemocytes and fat bodies. In the present study, we cloned the BmPLA2 gene from fat body tissue of silkworm Bombyx mori, which has a total sequence of 1.031 kb with a 31.90 kDa protein. In silico results of BmPLA2 indicated that the protein has a putative WD40 conserved domain and its phylogeny tree clustered with Danaus plexippus species. We investigated the transcriptional expression in development stages and tissues. The highest expression of BmPLA2 was screened in fat body among the studied tissues of third day fifth instar larva, with a high expression on third day fifth instar larva followed by a depression of expression in the wandering stage of the fifth instar larva. The expression of BmPLA2 in female pupa was higher than that of male pupa. Our RNAi-mediated gene silencing results showed highest reduction of BmPLA2 expression in post-24 h followed by post-48 and post-72 h. The BmPLA2-RNAi larvae and pupa could be characterized by pharate adult lethality and underdevelopment. The phenotypic characters of fat body cells in RNAi-induced larva implied that BmPLA2 affects the metabolic functions of fat body tissue in silkworm Bombyx mori.
authors.affiliation: Institute of Sericulture and Apiculture, College of Animal Sciences, Zhejiang University, Hangzhou, 310058, China.
authors.forename: Chabungbam
authors.initials: C
authors.lastname: Orville Singh
authors.affiliation: Institute of Sericulture and Apiculture, College of Animal Sciences, Zhejiang University, Hangzhou, 310058, China.
authors.forename: Hu-Hu
authors.initials: HH
authors.lastname: Xin
authors.affiliation: Institute of Sericulture and Apiculture, College of Animal Sciences, Zhejiang University, Hangzhou, 310058, China.
authors.forename: Rui-Ting
authors.initials: RT
authors.lastname: Chen
authors.affiliation: Institute of Sericulture and Apiculture, College of Animal Sciences, Zhejiang University, Hangzhou, 310058, China.
authors.forename: Mei-Xian
authors.initials: MX
authors.lastname: Wang
authors.affiliation: Institute of Sericulture and Apiculture, College of Animal Sciences, Zhejiang University, Hangzhou, 310058, China.
authors.forename: Shuang
authors.initials: S
authors.lastname: Liang
authors.affiliation: Institute of Sericulture and Apiculture, College of Animal Sciences, Zhejiang University, Hangzhou, 310058, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Lu
authors.affiliation: Institute of Sericulture and Apiculture, College of Animal Sciences, Zhejiang University, Hangzhou, 310058, China.
authors.forename: Zi-Zheng
authors.initials: ZZ
authors.lastname: Cai
authors.affiliation: Institute of Sericulture and Apiculture, College of Animal Sciences, Zhejiang University, Hangzhou, 310058, China.
authors.forename: Yun-Gen
authors.initials: YG
authors.lastname: Miao
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D012831:Bombyx / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D003001:Cloning, Molecular; D017124:Conserved Sequence; D004734:Energy Metabolism; D005216:Fat Body / Q000166:cytology / Q000378:metabolism*; D005260:Female; D018507:Gene Expression Regulation, Developmental; D019476:Insect Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D007814:Larva / Q000254:growth & development / Q000378:metabolism; D008297:Male; D008969:Molecular Sequence Data; D010641:Phenotype; D017434:Protein Structure, Tertiary; D011679:Pupa / Q000254:growth & development / Q000378:metabolism; D014158:Transcription, Genetic
chemical_list: D019476:Insect Proteins
keywords: BmPLA2; cloning and expression; dsRNA interference; fat body tissue; silkworm