title: CEACAM1 confers resistance toward oxygen-induced vessel damage in a mouse model of retinopathy of prematurity.
abstract: OBJECTIVE
  To determine a functional role for the carcinoembryonic antigen-related cell-adhesion molecule 1 (CEACAM1) in retinal neovascularization in a mouse model of oxygen-induced retinopathy (OIR).
  
  
  METHODS
  In a 21/75/21% OIR mouse model, retinal neovascularization was compared in wild-type and CEACAM1-deficient mice. Animals were housed under normoxic conditions until postnatal day 7, followed by exposure to 75% oxygen for 5 days, and further housing under normoxic conditions. Retinal vascular anatomy, vaso-obliteration, neovascularization, and tuft formation were characterized and quantified in retinal flat-mounts from untreated mice and from experimental mice during and at different time points after exposure to high oxygen levels. The vascular network was stained with fluorescently labeled isolectin B4.
  
  
  RESULTS
  Mice deficient in CEACAM1 did not present any apparent abnormalities in their postnatal retinal vascular development under normoxic housing conditions. However, after hyperoxia and under relative hypoxic conditions, retinal neovascularization and tuft formation were aggravated in the mutant. Congruently, revascularization and vessel maturation were delayed in CEACAM1-deficient mice whereas in wild-type mice, tuft regression and vascular remodeling occurred efficiently after exposure to high oxygen levels.
  
  
  CONCLUSIONS
  Our report describes a functional role for CEACAM1 in retinal neovascularization in a mouse model of OIR. This is the first study demonstrating that CEACAM1 enhances vascular remodeling and tuft regression by increasing endothelial resistance to alterations in oxygen tension, thus accelerating vascular recovery after systemic hypoxia.
authors.affiliation: Institute of Clinical Chemistry, Center for Diagnostics, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.
authors.forename: Peter
authors.initials: P
authors.lastname: Ludewig
authors.affiliation: Department of Ophthalmology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.
authors.forename: Kai
authors.initials: K
authors.lastname: Flachsbarth
authors.affiliation: Institute of Experimental Immunology and Hepatology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.
authors.forename: Claudia
authors.initials: C
authors.lastname: Wegscheid
authors.affiliation: Institute of Experimental Immunology and Hepatology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.
authors.forename: Gisa
authors.initials: G
authors.lastname: Tiegs
authors.affiliation: Department of Ophthalmology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.
authors.forename: Gisbert
authors.initials: G
authors.lastname: Richard
authors.affiliation: Institute of Clinical Chemistry, Center for Diagnostics, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.
authors.forename: Christoph
authors.initials: C
authors.lastname: Wagener
authors.affiliation: Department of Ophthalmology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.
authors.forename: Udo
authors.initials: U
authors.lastname: Bartsch
authors.affiliation: Institute of Clinical Chemistry, Center for Diagnostics, University Medical Center Hamburg-Eppendorf, Hamburg, Germany Institute of Experimental Immunology and Hepatology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.
authors.forename: Andrea Kristina
authors.initials: AK
authors.lastname: Horst
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000502:physiology*; D015815:Cell Adhesion Molecules / Q000172:deficiency / Q000502:physiology*; D004195:Disease Models, Animal; D018496:Hyperoxia / Q000503:physiopathology; D051379:Mice; D008810:Mice, Inbred C57BL; D010100:Oxygen; D015861:Retinal Neovascularization / Q000139:chemically induced / Q000503:physiopathology*; D012171:Retinal Vessels / Q000187:drug effects / Q000503:physiopathology; D012178:Retinopathy of Prematurity / Q000139:chemically induced / Q000503:physiopathology*
chemical_list: D015703:Antigens, CD; C069847:CD66 antigens; D015815:Cell Adhesion Molecules; D010100:Oxygen
keywords: neovascularization; retinal ischemia; retinal vasculature; retinopathy of prematurity; vascular remodeling