title: In vivo imaging of mitochondria in intact zebrafish larvae.
abstract: Visualizing neuronal mitochondria in a living, intact mammalian organism is a challenge that can be overcome in zebrafish larvae, which are highly accessible for optical imaging and genetic manipulation. Here, we detail an approach to visualize neuronal mitochondria in sensory Rohon-Beard axons, which allows quantitatively measuring mitochondrial shape, dynamics, and transport in vivo. This provides a useful assay for basic studies exploring the behavior of neuronal mitochondria in their natural habitat, for revealing the influence that disease-related alterations have on this behavior and for testing pharmacological compounds and genetic manipulations that might ameliorate disease-related mitochondrial phenotypes in neurons.
authors.affiliation: Adolf-Butenandt-Institute, Biochemistry, Ludwig-Maximilians-University, Munich, Germany; German Center for Neurodegenerative Diseases (DZNE), Munich, Germany.
authors.forename: Dominik
authors.initials: D
authors.lastname: Paquet
authors.affiliation: Munich Center for Systems Neurology (SyNergy), Munich, Germany.
authors.forename: Gabriela
authors.initials: G
authors.lastname: Plucińska
authors.affiliation: German Center for Neurodegenerative Diseases (DZNE), Munich, Germany; Munich Center for Systems Neurology (SyNergy), Munich, Germany; Institute of Neuronal Cell Biology, Technische Universität München, Munich, Germany. Electronic address: thomas.misgeld@lrz.tu-muenchen.de.
authors.forename: Thomas
authors.initials: T
authors.lastname: Misgeld
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D001370:Axonal Transport; D001369:Axons; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D001330:Electronic Data Processing; D004625:Embryo, Nonmammalian / Q000166:cytology; D007091:Image Processing, Computer-Assisted; D007814:Larva / Q000166:cytology*; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D008856:Microscopy, Fluorescence / Q000295:instrumentation / Q000379:methods*; D008928:Mitochondria / Q000502:physiology*; D011984:Sensory Receptor Cells / Q000166:cytology*; D015027:Zebrafish / Q000196:embryology / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; D008164:Luminescent Proteins; C054562:yellow fluorescent protein, Bacteria
keywords: Axonal transport; In vivo imaging; Microscopy; Zebrafish