title: Healing effect of pistacia atlantica fruit oil extract in acetic Acid-induced colitis in rats.
abstract: BACKGROUND
  Considering the anti-oxidant properties of Pistacia atlantica and lack of data regarding its efficacy in the treatment of ulcerative colitis, this study aims at investigating the effect of the Pistacia atlantica fruit extract in treating experimentally induced colitis in a rat model.
  
  
  METHODS
  Seventy male Sprague-Dawley rats (weighing 220±20 g) were used. All rats fasted 24 hours before the experimental procedure. The rats were randomly divided into 7 groups, each containing 10 induced colitis with 2ml acetic acid (3%). Group 1 (Asacol), group 2 (base gel) and group 7 (without treatment) were assigned as control groups. Group 3 (300 mg/ml) and group 4 (600 mg/ml) received Pistacia atlantica fruit orally. Group 5 (10% gel) and group 6 (20% gel) received Pistacia atlantica in the form of gel as enema. Macroscopic, histopathological examination and MDA measurement were carried out.
  
  
  RESULTS
  All groups revealed significant macroscopic healing in comparison with group 7 (P<0.001). Regarding microscopic findings in the treatment groups compared with group 7, the latter group differed significantly with groups 1, 2, 4 and 6 (P<0.001). There was a significant statistical difference in MDA scores of the seven treatment groups (F(5,54)=76.61, P<0.001). Post-hoc comparisons indicated that the mean±SD score of Asacol treated group (1.57±0.045) was not significantly different from groups 4 (1.62±0.024) and 6 (1.58±0.028).
  
  
  CONCLUSIONS
  Our study showed that a high dose of Pistacia atlantica fruit oil extract, administered orally and rectally can improve colitis physiologically and pathologically in a rat model, and may be efficient for ulcerative colitis.
authors.affiliation: Shiraz Burn Research Center, Colorectal Research Center, Faghihi Hospital, Shiraz University of Medical Sciences, Shiraz, Iran;
authors.forename: Nader
authors.initials: N
authors.lastname: Tanideh
authors.affiliation: Student Research Committee, Shiraz University of Medical Sciences, Shiraz, Iran;
authors.forename: Samira
authors.initials: S
authors.lastname: Masoumi
authors.affiliation: Colorectal Research Center, Faghihi Hospital, Shiraz University of Medical Sciences, Shiraz, Iran;
authors.forename: Massood
authors.initials: M
authors.lastname: Hosseinzadeh
authors.affiliation: Colorectal Research Center, Faghihi Hospital, Shiraz University of Medical Sciences, Shiraz, Iran;
authors.forename: Ali Reza
authors.initials: AR
authors.lastname: Safarpour
authors.affiliation: Student Research Committee, Veterinary School, Shiraz University, Shiraz, Iran;
authors.forename: Hoda
authors.initials: H
authors.lastname: Erjaee
authors.affiliation: Center for Comparative and Experimental Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Omid
authors.initials: O
authors.lastname: Koohi-Hosseinabadi
authors.affiliation: Colorectal Research Center, Faghihi Hospital, Shiraz University of Medical Sciences, Shiraz, Iran;
authors.forename: Salar
authors.initials: S
authors.lastname: Rahimikazerooni
keywords: Colitis; Enema; Rat