title: Aberrant expression of Xist in aborted porcine fetuses derived from somatic cell nuclear transfer embryos.
abstract: Cloned pigs generated by somatic cell nuclear transfer (SCNT) show a greater ratio of early abortion during mid-gestation than normal controls. X-linked genes have been demonstrated to be important for the development of cloned embryos. To determine the relationship between the expression of X-linked genes and abortion of cloned porcine fetuses, the expression of X-linked genes were investigated by quantitative real-time polymerase chain reaction (q-PCR) and the methylation status of Xist DMR was performed by bisulfate-specific PCR (BSP). q-PCR analysis indicated that there was aberrant expression of X-linked genes, especially the upregulated expression of Xist in both female and male aborted fetuses compared to control fetuses. Results of BSP suggested that hypomethylation of Xist occurred in aborted fetuses, whether male or female. These results suggest that the abnormal expression of Xist may be associated with the abortion of fetuses derived from somatic cell nuclear transfer embryos.
authors.affiliation: Jilin Provincial Key Laboratory of Animal Embryo Engineering, College of Animal Sciences, Jilin University, Changchun 130062, China. peguin119@126.com.
authors.forename: Lin
authors.initials: L
authors.lastname: Yuan
authors.affiliation: Jilin Provincial Key Laboratory of Animal Embryo Engineering, College of Animal Sciences, Jilin University, Changchun 130062, China. walfers@163.com.
authors.forename: Anfeng
authors.initials: A
authors.lastname: Wang
authors.affiliation: Jilin Provincial Key Laboratory of Animal Embryo Engineering, College of Animal Sciences, Jilin University, Changchun 130062, China. lengyinfei@126.com.
authors.forename: Chaogang
authors.initials: C
authors.lastname: Yao
authors.affiliation: Jilin Provincial Key Laboratory of Animal Embryo Engineering, College of Animal Sciences, Jilin University, Changchun 130062, China. huangyongye88@163.com.
authors.forename: Yongye
authors.initials: Y
authors.lastname: Huang
authors.affiliation: Jilin Provincial Key Laboratory of Animal Embryo Engineering, College of Animal Sciences, Jilin University, Changchun 130062, China. dff1125@gmail.com.
authors.forename: Feifei
authors.initials: F
authors.lastname: Duan
authors.affiliation: Jilin Provincial Key Laboratory of Animal Embryo Engineering, College of Animal Sciences, Jilin University, Changchun 130062, China. lvqingyan123@163.com.
authors.forename: Qinyan
authors.initials: Q
authors.lastname: Lv
authors.affiliation: Jilin Provincial Key Laboratory of Animal Embryo Engineering, College of Animal Sciences, Jilin University, Changchun 130062, China. wang_dong_xu@163.com.
authors.forename: Dongxu
authors.initials: D
authors.lastname: Wang
authors.affiliation: Jilin Provincial Key Laboratory of Animal Embryo Engineering, College of Animal Sciences, Jilin University, Changchun 130062, China. ouyh@jlu.edu.cn.
authors.forename: Hongsheng
authors.initials: H
authors.lastname: Ouyang
authors.affiliation: Jilin Provincial Key Laboratory of Animal Embryo Engineering, College of Animal Sciences, Jilin University, Changchun 130062, China. lizj_1998@jlu.edu.cn.
authors.forename: Zhanjun
authors.initials: Z
authors.lastname: Li
authors.affiliation: Jilin Provincial Key Laboratory of Animal Embryo Engineering, College of Animal Sciences, Jilin University, Changchun 130062, China. lai_liangxue@gibh.ac.cn.
authors.forename: Liangxue
authors.initials: L
authors.lastname: Lai
mesh_terms: D000022:Abortion, Spontaneous / Q000235:genetics*; D000818:Animals; D019175:DNA Methylation / Q000235:genetics; D004622:Embryo, Mammalian / Q000378:metabolism*; D005260:Female; D005333:Fetus / Q000378:metabolism*; D005786:Gene Expression Regulation; D005838:Genotype; D008297:Male; D053652:Nuclear Transfer Techniques; D011247:Pregnancy; D062085:RNA, Long Noncoding / Q000235:genetics* / Q000378:metabolism; D034421:Sus scrofa; D014158:Transcription, Genetic
chemical_list: D062085:RNA, Long Noncoding; C077885:XIST non-coding RNA