title: Refolding additive, dimethylbenzylammonium propane sulfonate (NDSB- 256), accelerates gly-pro cis-trans isomerization.
abstract: Proline cis-trans isomerization plays a key role in the rate-determining steps of protein folding, and many different peptide-proline cis-trans isomerases (PPIases) catalyze this reaction. The acceleration of isomerization would be beneficial for in vitro refolding of protein preparations for industrial and research purposes. So we analyzed whether low-molecular-weight compounds that have been reported to enhance protein refolding have the activity to accelerate the isomerization. To evaluate the effects of chemicals on the isomerization rate, we set up a new NMR (EXSY) method that is invulnerable to their inhibitory activity, if any, and to their large NMR signals. With this method, we found that dimethylbenzylammonium propane sulfonate (NDSB-256) increase the isomerization rate in a concentration-dependent manner for the first time. Acceleration by imidazole (suggested but not experimentally confirmed) was also demonstrated. Arginine, a most popular refolding additive, did not show any significant effects on the isomerization reaction as expected.
authors.forename: Haimei
authors.initials: H
authors.lastname: Wang
authors.forename: Kazuo
authors.initials: K
authors.lastname: Hosoda
authors.forename: Shin-Ichi
authors.initials: S
authors.lastname: Terawaki
authors.affiliation: 1-5-1 Tenjin-cho, Kiryu, Gunma 376-8515, Japan. kwakamats@gunma-u.ac.jp.
authors.forename: Kaori
authors.initials: K
authors.lastname: Wakamatsu
mesh_terms: D001120:Arginine / Q000494:pharmacology; D001622:Betaine / Q000031:analogs & derivatives* / Q000494:pharmacology; D007093:Imidazoles / Q000494:pharmacology*; D007536:Isomerism; D008956:Models, Chemical; D008970:Molecular Weight; D019906:Nuclear Magnetic Resonance, Biomolecular; D010455:Peptides / Q000737:chemistry*; D011392:Proline / Q000737:chemistry*; D058849:Protein Refolding
chemical_list: D007093:Imidazoles; C114724:NDSB 256; D010455:Peptides; D001622:Betaine; C029899:imidazole; D001120:Arginine; D011392:Proline