title: Rapid and accurate determination of the lignin content of lignocellulosic biomass by solid-state NMR.
abstract: Biofuels and biomaterials, produced from lignocellulosic feedstock, require facile access to cellulose and hemicellulose to be competitive with petroleum processing and sugar-based fermentation. Physical-chemical barriers resulting from lignin complicates the hydrolysis biomass into fermentable sugars. Thus, the amount of lignin within a substrate is critical in determining biomass processing. The application of13C cross-polarization, magic-angle spinning, and solid-state nuclear magnetic resonance for the direct quantification of lignin content in biomass is examined. Using a standard curve constructed from pristine lignin and cellulose, the lignin content of a biomass sample is accurately determined through direct measurement without chemical or enzymatic pre-treatment.
authors.affiliation: Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, New York 12180, USA.
authors.forename: Li
authors.initials: L
authors.lastname: Fu
authors.affiliation: Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, New York 12180, USA.
authors.forename: Scott A
authors.initials: SA
authors.lastname: McCallum
authors.affiliation: Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, New York 12180, USA.
authors.forename: Jianjun
authors.initials: J
authors.lastname: Miao
authors.affiliation: Ecovative Design, LLC, 70 Cohoes Ave, Green Island, New York 12183, USA.
authors.forename: Courtney
authors.initials: C
authors.lastname: Hart
authors.affiliation: Ecovative Design, LLC, 70 Cohoes Ave, Green Island, New York 12183, USA.
authors.forename: Gregory J
authors.initials: GJ
authors.lastname: Tudryn
authors.affiliation: Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, New York 12180, USA.
authors.forename: Fuming
authors.initials: F
authors.lastname: Zhang
authors.affiliation: Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, New York 12180, USA ; Departments of Chemistry and Chemical Biology, Chemical and Biological Engineering, and Biology and Biomedical Engineering Rensselaer Polytechnic Institute, Troy, New York 12180, USA.
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Linhardt
keywords: biomass; cellulose; lignin; solid-state NMR