title: Gender differences in corydaline pharmacokinetics in rats.
abstract: 1. Corydaline, an isoquinoline alkaloid, is one of the major active constituents in a new prokinetic botanical agent, DA-9701. It has been recommended that preclinical pharmacokinetic studies of natural medicines include both genders. Therefore, in this study, the pharmacokinetics of corydaline in male and female rats was evaluated following intravenous and oral administration of pure corydaline or DA-9701. 2. After intravenous administration of corydaline, the area under the plasma concentration-time curve (AUC) was significantly greater (by 46.4%) in female rats compared to male rats due to a 29.3% reduction in non-renal clearance in female rats. The gender difference in corydaline hepatic metabolic clearance was supported by a significantly slower metabolism of corydaline in hepatic microsomes of female rats mediated via male-specific (CYP2C11 and CYP3A2) or male-dominant (CYP3A1) CYP isozymes. 3. Following oral administration of pure corydaline or DA-9701, the AUC and Cmax values of corydaline in female rats were significantly greater (by 793% and 466% increase for corydaline administration or by 501% and 143% increase for DA-9701 administration) than in male rats. Greater F values of corydaline in female rats could be due to smaller hepatic first-pass extraction as a result of slower hepatic metabolism of corydaline. 4. However, we observed a comparable disappearance of corydaline in male and female human liver microsomes, consistent with little gender difference in CYP2C9 and CYP3A activities in humans compared to that in rats. Thus, gender differences in corydaline metabolism are not expected to occur in humans.
authors.affiliation: College of Pharmacy and Integrated Research Institute of Pharmaceutical Sciences, The Catholic University of Korea , Bucheon , Republic of Korea and.
authors.forename: Ji Won
authors.initials: JW
authors.lastname: Jung
authors.forename: Mi Ran
authors.initials: MR
authors.lastname: Choi
authors.forename: Yong Sam
authors.initials: YS
authors.lastname: Kwon
authors.forename: Jin Seok
authors.initials: JS
authors.lastname: Jeong
authors.forename: Miwon
authors.initials: M
authors.lastname: Son
authors.forename: Hee Eun
authors.initials: HE
authors.lastname: Kang
mesh_terms: D000284:Administration, Oral; D000818:Animals; D001600:Berberine Alkaloids / Q000008:administration & dosage / Q000097:blood / Q000737:chemistry / Q000493:pharmacokinetics*; D005260:Female; D006801:Humans; D007275:Injections, Intravenous; D008297:Male; D008862:Microsomes, Liver / Q000378:metabolism; D011485:Protein Binding; D017207:Rats, Sprague-Dawley; D012727:Sex Characteristics
chemical_list: D001600:Berberine Alkaloids; C452799:corydaline
keywords: CYP2C; CYP3A; Corydaline; DA-9701; gender; pharmacokinetics; rats