title: A peptide from human semenogelin I self-assembles into a pH-responsive hydrogel.
abstract: The peptide GSFSIQYTYHV derived from human semenogelin I forms a transparent hydrogel through spontaneous self-assembly in water at neutral pH. Linear rheology measurements demonstrate that the gel shows a dominating elastic response over a large frequency interval. CD, fluorescence and FTIR spectroscopy and cryo-TEM studies imply long fibrillar aggregates of extended β-sheet. Dynamic light scattering data indicate that the fibril lengths are of the order of micrometers. Time-dependent thioflavin T fluorescence shows that fibril formation by GSFSIQYTYHV is a nucleated reaction. The peptide may serve as basis for development of smart biomaterials of low immunogenicity suitable for biomedical applications, including drug delivery and wound healing.
authors.affiliation: Biochemistry and Structural Biology, Lund University, P O Box 124, SE-221 00 Lund, Sweden. sara.linse@biochemistry.lu.se.
authors.forename: B
authors.initials: B
authors.lastname: Frohm
authors.forename: J E
authors.initials: JE
authors.lastname: DeNizio
authors.forename: D S M
authors.initials: DS
authors.lastname: Lee
authors.forename: L
authors.initials: L
authors.lastname: Gentile
authors.forename: U
authors.initials: U
authors.lastname: Olsson
authors.forename: J
authors.initials: J
authors.lastname: Malm
authors.forename: K S
authors.initials: KS
authors.lastname: Akerfeldt
authors.forename: S
authors.initials: S
authors.lastname: Linse
mesh_terms: D006801:Humans; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D010455:Peptides / Q000737:chemistry*; D017433:Protein Structure, Secondary; D012212:Rheology; D029607:Seminal Vesicle Secretory Proteins / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D010455:Peptides; D029607:Seminal Vesicle Secretory Proteins; C424482:seminal vesicle-specific antigen; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate