title: Crosslinked collagen-gelatin-hyaluronic acid biomimetic film for cornea tissue engineering applications.
abstract: Cornea disease may lead to blindness and keratoplasty is considered as an effective treatment method. However, there is a severe shortage of donor corneas worldwide. This paper presents the crosslinked collagen (Col)-gelatin (Gel)-hyaluronic acid (HA) films developed by making use of 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide (EDC) and N-hydroxysuccinimide (NHS) as the crosslinker. The test results on the physical and biological properties indicate that the CGH631 film (the mass ratio of Col:Gel:HA=6:3:1) has appropriate optical performance, hydrophilicity and mechanical properties. The diffusion properties of the CGH631 film to NaCl and tryptophan are also satisfactory and the measured data are 2.43×10(-6) cm(2)/s and 7.97×10(-7) cm(2)/s, respectively. In addition, cell viability studies demonstrate that the CGH631 film has good biocompatibility, on which human corneal epithelial cells attached and proliferated well. This biocompatible film may have potential use in cornea tissue engineering.
authors.affiliation: School of Materials Science and Engineering, South China University of Technology, Guangzhou 510641, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.affiliation: School of Materials Science and Engineering, South China University of Technology, Guangzhou 510641, China; National Engineering Research Center for Tissue Restoration and Reconstruction, Guangzhou 510006, China; Guangdong Province Key Laboratory of Biomedical Engineering, South China University of Technology, Guangzhou 510006, China. Electronic address: psliren@scut.edu.cn.
authors.forename: Li
authors.initials: L
authors.lastname: Ren
authors.affiliation: School of Materials Science and Engineering, South China University of Technology, Guangzhou 510641, China; National Engineering Research Center for Tissue Restoration and Reconstruction, Guangzhou 510006, China; Guangdong Province Key Laboratory of Biomedical Engineering, South China University of Technology, Guangzhou 510006, China. Electronic address: imwangyj@163.com.
authors.forename: Yingjun
authors.initials: Y
authors.lastname: Wang
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry* / Q000494:pharmacology; D002234:Carbodiimides / Q000737:chemistry; D002448:Cell Adhesion / Q000187:drug effects; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D003094:Collagen / Q000737:chemistry*; D003315:Cornea / Q000166:cytology; D004058:Diffusion; D005780:Gelatin / Q000737:chemistry*; D006801:Humans; D006820:Hyaluronic Acid / Q000737:chemistry*; D057927:Hydrophobic and Hydrophilic Interactions; D013388:Succinimides / Q000737:chemistry; D023822:Tissue Engineering
chemical_list: C557098:1-ethyl-3-(3-dimethylaminoisopropyl) carbodiimide; D001672:Biocompatible Materials; D002234:Carbodiimides; D013388:Succinimides; D005780:Gelatin; D006820:Hyaluronic Acid; D003094:Collagen; C001426:N-hydroxysuccinimide