title: Strategies to increase genome editing frequencies and to facilitate the identification of edited cells.
abstract: The power of genome editing is increasingly recognized as it has become more accessible to a wide range of scientists and a wider range of uses has been reported. Nonetheless, an important practical aspect of the strategy is develop methods to increase the frequency of genome editing or methods that enrich for genome-edited cells such that they can be more easily identified. This chapter discusses several different approaches including the use of cold-shock, exonucleases, surrogate markers, specialized donor vectors, and oligonucleotides to enhance the frequency of genome editing or to facilitate the identification of genome-edited cells.
authors.affiliation: Hematology/Oncology/Stem Cell Transplantation/Cancer Biology, Department of Pediatrics, School of Medicine, Stanford University, Lorry Lokey Stem Cell Research Building, G3045, MC 5462, 269 Campus Drive, Stanford, CA, 94305, USA, mporteus@stanford.edu.
authors.forename: Matthew
authors.initials: M
authors.lastname: Porteus
mesh_terms: D059766:DNA End-Joining Repair; D004706:Endodeoxyribonucleases / Q000235:genetics / Q000378:metabolism; D005090:Exodeoxyribonucleases / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D018390:Gene Targeting; D017930:Genes, Reporter; D005818:Genetic Engineering / Q000379:methods*; D059765:Homologous Recombination; D006801:Humans; D016254:Mutagenesis, Insertional / Q000379:methods; D009841:Oligonucleotides / Q000235:genetics; D059010:Single-Cell Analysis / Q000379:methods*
chemical_list: D009841:Oligonucleotides; D004706:Endodeoxyribonucleases; D005090:Exodeoxyribonucleases