title: Detection of chikungunya virus antigen by a novel rapid immunochromatographic test.
abstract: Chikungunya fever is a mosquito-borne disease of key public health importance in tropical and subtropical countries. Although severe joint pain is the most distinguishing feature of chikungunya fever, diagnosis remains difficult because the symptoms of chikungunya fever are shared by many pathogens, including dengue fever. The present study aimed to develop a new immunochromatographic diagnosis test for the detection of chikungunya virus antigen in serum. Mice were immunized with isolates from patients with Thai chikungunya fever, East/Central/South African genotype, to produce mouse monoclonal antibodies against chikungunya virus. Using these monoclonal antibodies, a new diagnostic test was developed and evaluated for the detection of chikungunya virus. The newly developed diagnostic test reacted with not only the East/Central/South African genotype but also with the Asian and West African genotypes of chikungunya virus. Testing of sera from patients suspected to have chikungunya fever in Thailand (n = 50), Laos (n = 54), Indonesia (n = 2), and Senegal (n = 6) revealed sensitivity, specificity, and real-time PCR (RT-PCR) agreement values of 89.4%, 94.4%, and 91.1%, respectively. In our study using serial samples, a new diagnostic test showed high agreement with the RT-PCR within the first 5 days after onset. A rapid diagnostic test was developed using mouse monoclonal antibodies that react with chikungunya virus envelope proteins. The diagnostic accuracy of our test is clinically acceptable for chikungunya fever in the acute phase.
authors.affiliation: Mahidol-Osaka Center for Infectious Diseases (MOCID), Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand Department of Virology, Research Institute for Microbial Diseases, Osaka University, Osaka, Japan tamaoka@biken.osaka-u.ac.jp.
authors.forename: Tamaki
authors.initials: T
authors.lastname: Okabayashi
authors.affiliation: Department of Virology, Research Institute for Microbial Diseases, Osaka University, Osaka, Japan.
authors.forename: Tadahiro
authors.initials: T
authors.lastname: Sasaki
authors.affiliation: Mahidol-Osaka Center for Infectious Diseases (MOCID), Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand Center for Vaccine Development, Mahidol University, Nakhon Pathom, Thailand.
authors.forename: Promsin
authors.initials: P
authors.lastname: Masrinoul
authors.affiliation: Mahidol-Osaka Center for Infectious Diseases (MOCID), Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
authors.forename: Nantarat
authors.initials: N
authors.lastname: Chantawat
authors.affiliation: Center for Vaccine Development, Mahidol University, Nakhon Pathom, Thailand.
authors.forename: Sutee
authors.initials: S
authors.lastname: Yoksan
authors.affiliation: Center for Vaccine Development, Mahidol University, Nakhon Pathom, Thailand.
authors.forename: Narong
authors.initials: N
authors.lastname: Nitatpattana
authors.affiliation: Division of Infectious Disease, Department of Internal Medicine, Faculty of Medicine, Prince of Songkla University, Hat Yai, Thailand.
authors.forename: Sarunyou
authors.initials: S
authors.lastname: Chusri
authors.affiliation: Department of Medical Entomology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
authors.forename: Ronald E
authors.initials: RE
authors.lastname: Morales Vargas
authors.affiliation: Institute Pasteur du Laos, Vientiane, Lao People's Democratic Republic.
authors.forename: Marc
authors.initials: M
authors.lastname: Grandadam
authors.affiliation: Institute Pasteur du Laos, Vientiane, Lao People's Democratic Republic.
authors.forename: Paul T
authors.initials: PT
authors.lastname: Brey
authors.affiliation: Indonesia-Japan Collaboration Research Center for Emerging and Re-emerging Infectious Diseases, Institute of Tropical Diseases, Airlangga University, Surabaya, Indonesia.
authors.forename: Soegeng
authors.initials: S
authors.lastname: Soegijanto
authors.affiliation: Indonesia-Japan Collaboration Research Center for Emerging and Re-emerging Infectious Diseases, Institute of Tropical Diseases, Airlangga University, Surabaya, Indonesia.
authors.forename: Kris Cahyo
authors.initials: KC
authors.lastname: Mulyantno
authors.affiliation: Indonesia-Japan Collaboration Research Center for Emerging and Re-emerging Infectious Diseases, Institute of Tropical Diseases, Airlangga University, Surabaya, Indonesia.
authors.forename: Siti
authors.initials: S
authors.lastname: Churrotin
authors.affiliation: Indonesia-Japan Collaboration Research Center for Emerging and Re-emerging Infectious Diseases, Institute of Tropical Diseases, Airlangga University, Surabaya, Indonesia Center for Infectious Diseases, Kobe University Graduate School of Medicine, Hyogo, Japan.
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Kotaki
authors.affiliation: Arboviruses and Viral Hemorrhagic Fever Unit, Institute Pasteur de Dakar, Dakar, Senegal.
authors.forename: Oumar
authors.initials: O
authors.lastname: Faye
authors.affiliation: Arboviruses and Viral Hemorrhagic Fever Unit, Institute Pasteur de Dakar, Dakar, Senegal.
authors.forename: Ousmane
authors.initials: O
authors.lastname: Faye
authors.affiliation: Arboviruses and Viral Hemorrhagic Fever Unit, Institute Pasteur de Dakar, Dakar, Senegal.
authors.forename: Abdourahmane
authors.initials: A
authors.lastname: Sow
authors.affiliation: Arboviruses and Viral Hemorrhagic Fever Unit, Institute Pasteur de Dakar, Dakar, Senegal.
authors.forename: Amadou Alpha
authors.initials: AA
authors.lastname: Sall
authors.affiliation: Mahidol-Osaka Center for Infectious Diseases (MOCID), Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
authors.forename: Orapim
authors.initials: O
authors.lastname: Puiprom
authors.affiliation: Mahidol-Osaka Center for Infectious Diseases (MOCID), Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
authors.forename: Panjaporn
authors.initials: P
authors.lastname: Chaichana
authors.affiliation: Department of Virology, Research Institute for Microbial Diseases, Osaka University, Osaka, Japan.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Kurosu
authors.affiliation: Alfresa Pharma Corporation, Osaka, Japan.
authors.forename: Seiji
authors.initials: S
authors.lastname: Kato
authors.affiliation: Alfresa Pharma Corporation, Osaka, Japan Arkray Incorporated, Kyoto, Japan.
authors.forename: Mieko
authors.initials: M
authors.lastname: Kosaka
authors.affiliation: Center of Excellence for Antibody Research, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
authors.forename: Pongrama
authors.initials: P
authors.lastname: Ramasoota
authors.affiliation: Department of Virology, Research Institute for Microbial Diseases, Osaka University, Osaka, Japan.
authors.forename: Kazuyoshi
authors.initials: K
authors.lastname: Ikuta
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000914:Antibodies, Viral / Q000276:immunology; D000956:Antigens, Viral / Q000097:blood*; D065632:Chikungunya Fever / Q000175:diagnosis* / Q000821:virology; D002646:Chikungunya virus / Q000302:isolation & purification*; D002846:Chromatography, Affinity / Q000379:methods*; D003955:Diagnostic Tests, Routine / Q000379:methods*; D006801:Humans; D007214:Indonesia; D008807:Mice, Inbred BALB C; D012675:Senegal; D012680:Sensitivity and Specificity; D044967:Serum / Q000821:virology*; D013785:Thailand; D013997:Time Factors
chemical_list: D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000956:Antigens, Viral