title: 808 nm driven Nd3+-sensitized upconversion nanostructures for photodynamic therapy and simultaneous fluorescence imaging.
abstract: The in vivo biological applications of upconversion nanoparticles (UCNPs) prefer excitation at 700-850 nm, instead of 980 nm, due to the absorption of water. Recent approaches in constructing robust Nd(3+) doped UCNPs with 808 nm excitation properties rely on a thick Nd(3+) sensitized shell. However, for the very important and popular Förster resonance energy transfer (FRET)-based applications, such as photodynamic therapy (PDT) or switchable biosensors, this type of structure has restrictions resulting in a poor energy transfer. In this work, we have designed a NaYF4:Yb/Ho@NaYF4:Nd@NaYF4 core-shell-shell nanostructure. We have proven that this optimal structure balances the robustness of the upconversion emission and the FRET efficiency for FRET-based bioapplications. A proof of the concept was demonstrated for photodynamic therapy and simultaneous fluorescence imaging of HeLa cells triggered by 808 nm light, where low heating and a high PDT efficacy were achieved.
authors.affiliation: State Key Laboratory of Luminescence and Applications, Changchun Institute of Optics, Fine Mechanics and Physics, Chinese Academy of Sciences, Changchun 130033, China. xgkong14@ciomp.ac.cn.
authors.forename: Dan
authors.initials: D
authors.lastname: Wang
authors.forename: Bin
authors.initials: B
authors.lastname: Xue
authors.forename: Xianggui
authors.initials: X
authors.lastname: Kong
authors.forename: Langping
authors.initials: L
authors.lastname: Tu
authors.forename: Xiaomin
authors.initials: X
authors.lastname: Liu
authors.forename: Youlin
authors.initials: Y
authors.lastname: Zhang
authors.forename: Yulei
authors.initials: Y
authors.lastname: Chang
authors.forename: Yongshi
authors.initials: Y
authors.lastname: Luo
authors.forename: Huiying
authors.initials: H
authors.lastname: Zhao
authors.forename: Hong
authors.initials: H
authors.lastname: Zhang
mesh_terms: D003287:Contrast Media / Q000138:chemical synthesis* / Q000528:radiation effects / Q000627:therapeutic use; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods; D008027:Light; D008422:Materials Testing; D053768:Metal Nanoparticles / Q000528:radiation effects* / Q000627:therapeutic use* / Q000648:ultrastructure; D008856:Microscopy, Fluorescence / Q000379:methods*; D009354:Neodymium / Q000737:chemistry* / Q000528:radiation effects; D010778:Photochemotherapy / Q000379:methods*
chemical_list: D003287:Contrast Media; D009354:Neodymium