title: The Dynamin 2 inhibitor Dynasore affects the actin filament distribution during mouse early embryo development.
abstract: Dynamin 2 is a large GTPase notably involved in clathrin-mediated endocytosis, cell migration and cytokinesis in mitosis. Our previous study identified that Dynamin 2 regulated polar body extrusion in mammalian oocytes, but its roles in early embryo development, remain elusive. Here, we report the critical roles of Dynamin 2 in mouse early embryo development. Dynamin 2 accumulated at the periphery of the blastomere during embryonic development. When Dynamin 2 activity was inhibited by Dynasore, embryos failed to cleave to the 2-cell or 4-cell stage. Moreover, the actin filament distribution and relative amount were aberrant in the treatment group. Similar results were observed when embryos were cultured with Dynasore at the 8-cell stage; the embryos failed to undergo compaction and develop to the morula stage, indicating a role of Dynamin 2 in embryo cytokinesis. Therefore, our data indicate that Dynamin 2 might participate in the early embryonic development through an actin-based cytokinesis.
authors.affiliation: College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Qiao-Chu
authors.initials: QC
authors.lastname: Wang
authors.forename: Jun
authors.initials: J
authors.lastname: Liu
authors.forename: Xing
authors.initials: X
authors.lastname: Duan
authors.forename: Xiang-Shun
authors.initials: XS
authors.lastname: Cui
authors.forename: Nam-Hyung
authors.initials: NH
authors.lastname: Kim
authors.forename: Bo
authors.initials: B
authors.lastname: Xiong
authors.forename: Shao-Chen
authors.initials: SC
authors.lastname: Sun
mesh_terms: D008841:Actin Cytoskeleton / Q000378:metabolism; D000199:Actins / Q000378:metabolism*; D000818:Animals; D001755:Blastocyst / Q000166:cytology; D001757:Blastomeres / Q000648:ultrastructure; D048749:Cytokinesis; D034285:Dynamin II / Q000037:antagonists & inhibitors*; D005260:Female; D018507:Gene Expression Regulation, Developmental; D006835:Hydrazones / Q000737:chemistry*; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D009865:Oocytes / Q000166:cytology; D013997:Time Factors; D015053:Zygote / Q000378:metabolism
chemical_list: D000199:Actins; D006835:Hydrazones; C511794:N'-(3,4-dihydroxybenzylidene)-3-hydroxy-2-naphthahydrazide; D034285:Dynamin II