title: [Effect of andrographolide derivative Yanhuning on in vivo Candida albicans biofilms in rats].
abstract: OBJECTIVE
  To investigate the effect of andrographolide derivative Yanhuning (YHN) on Candida albicans biofilms in rats.
  
  
  METHODS
  The rat C. albicans biofilms subcutaneous catheter model was established by intraperitoneally injecting YHN (40, 20, 10, 5, 2.5 mg x kg (-1)), with the FLC (80 mg x kg(-1)) positive group as the control group. After 7 d, CFU counting and XTT assay were used to evaluate the effect of YHN on C. albicans biofllms in vivo. Scanning electron microscopy (SEM) was applied to observe the morphological changes in rat biofilms intervened by YHN. The real-time fluorescence quantification PCR was adopted to detect expressions of C. albicans adhesion-related genes, such as ALS1, ALS3, HWP1, EAP1 and MP65.
  
  
  RESULTS
  The YHN group showed much less CFUs on catheter pieces and lower XTT metabolic activity than the blank group, with dosage dependence. SEM also showed that YHN could obviously decrease C. albicans adhesion on subcutaneous catheters in rats. According to qRT-PCR's results, YHN can down-regulate expressions of ALS1, ALS3, HWP1, EAP1 and MP65.
  
  
  CONCLUSIONS
  YHN could inhibit C. albicans biofilms in rats.
authors.forename: Gao-Xiang
authors.initials: GX
authors.lastname: Shi
authors.forename: Yuan-Yuan
authors.initials: YY
authors.lastname: Yan
authors.forename: Jing
authors.initials: J
authors.lastname: Shao
authors.forename: Meng-Xiang
authors.initials: MX
authors.lastname: Zhang
authors.forename: Ke-Qiao
authors.initials: KQ
authors.lastname: Lu
authors.forename: Tian-Ming
authors.initials: TM
authors.lastname: Wang
authors.forename: Chang-Zhong
authors.initials: CZ
authors.lastname: Wang
mesh_terms: D000818:Animals; D018441:Biofilms / Q000187:drug effects* / Q000254:growth & development*; D002176:Candida albicans / Q000166:cytology / Q000187:drug effects* / Q000502:physiology*; D057785:Catheters / Q000382:microbiology; D002448:Cell Adhesion / Q000187:drug effects; D004224:Diterpenes / Q000737:chemistry* / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D051381:Rats
chemical_list: D004224:Diterpenes; C030419:andrographolide; C000593697:yanhuning