title: Effects of catecholamines on volemic replacement with saline solution and the impact on heart rate variability in rabbits subjected to hemorrhage. A study by spectral analysis.
abstract: OBJECTIVE
  To verify the effects of different catecholamines on volemic expansion and on the autonomic nervous system in rabbits that were subjected to hemorrhage.
  
  
  METHODS
  Twenty four rabbits subjected to hemorrhage (with a 25% loss of blood volume) and were randomly divided into four experimental groups: 1) HEMO Group underwent replacement with their own blood in an equal volume; 2) SS Group underwent replacement with saline solution (SS) in a volume that corresponded to three times the removed blood volume; 3) ISP Group underwent replacement with SS and isoprenaline; 4) FNL Group underwent replacement with SS and phenylephrine. Spectral Analysis of the heart rate and heart rate variability were performed from the recorded data. Hematocrit was measured throughout the experiment.
  
  
  RESULTS
  Replacement with SS and an α- or β-agonist did not produce differences in the intravascular retention compared to replacement with SS alone. An analysis of HRV showed that the FNL group maintained the LF/HF ratio better than ISP and SS.
  
  
  CONCLUSIONS
  No difference in vascular retention when α- or β- agonists were added to SS during post-hemorrhagic recovery. The animals in the FNL group maintained the integrity of the autonomic response within normal physiological standards during hemorrhagic stress.
authors.affiliation: Botucatu Medical School, Sao Paulo State University, Brazil.
authors.forename: José Mariano Soares de
authors.initials: JM
authors.lastname: Moraes
authors.affiliation: Medical School, University of Sao Paulo, Brazil.
authors.forename: Matheus Fachini
authors.initials: MF
authors.lastname: Vane
authors.affiliation: UNESP, Brazil.
authors.forename: Denise de Fátima
authors.initials: Dde F
authors.lastname: Rodrigues
authors.affiliation: Heart Institute, USP, Sao Paulo, SP, Brazil.
authors.forename: Cristiano Teixeira
authors.initials: CT
authors.lastname: Mostarda
authors.affiliation: Juiz de Fora Medical and Health School, Brazil.
authors.forename: Thiago Soares Mendes Moreira de
authors.initials: TS
authors.lastname: Moraes
authors.affiliation: UNESP, Brazil.
authors.forename: Lucas Fachini
authors.initials: LF
authors.lastname: Vane
authors.affiliation: Department of Anesthesiology, Botucatu Medical School, UNESP, Brazil.
authors.forename: Eliana Marisa
authors.initials: EM
authors.lastname: Ganem
authors.affiliation: Department of Anesthesiology, Rio de Janeiro Federal Fluminense University, Brazil.
authors.forename: Ismar Lima
authors.initials: IL
authors.lastname: Cavalcanti
authors.affiliation: Department of Anesthesiology, Botucatu Medical School, UNESP, Brazil.
authors.forename: Norma Sueli Pinheiro
authors.initials: NS
authors.lastname: Módolo
authors.affiliation: Department of Anesthesiology, Botucatu Medical School, UNESP, Brazil.
authors.forename: Luiz Antonio
authors.initials: LA
authors.lastname: Vane
mesh_terms: D058646:Adrenergic alpha-1 Receptor Agonists / Q000494:pharmacology; D000318:Adrenergic beta-Agonists / Q000494:pharmacology; D000818:Animals; D001341:Autonomic Nervous System / Q000187:drug effects; D001804:Blood Transfusion, Autologous; D001810:Blood Volume / Q000187:drug effects*; D002395:Catecholamines / Q000494:pharmacology*; D005583:Fourier Analysis; D006339:Heart Rate / Q000187:drug effects* / Q000502:physiology; D006400:Hematocrit; D006470:Hemorrhage / Q000209:etiology / Q000503:physiopathology* / Q000628:therapy; D007545:Isoproterenol / Q000494:pharmacology; D010656:Phenylephrine / Q000494:pharmacology; D011817:Rabbits; D011897:Random Allocation; D012016:Reference Values; D015203:Reproducibility of Results; D012965:Sodium Chloride / Q000494:pharmacology*; D013057:Spectrum Analysis; D013997:Time Factors
chemical_list: D058646:Adrenergic alpha-1 Receptor Agonists; D000318:Adrenergic beta-Agonists; D002395:Catecholamines; D010656:Phenylephrine; D012965:Sodium Chloride; D007545:Isoproterenol