title: Palmitoylated peptide 562-572 of luteinizing hormone receptor increases testosterone level in male rats.
abstract: We studied the effect of intraperitoneal and intratesticular administration of NKDTKIAKKNle-A(562-572) peptide and its palmotoylated analog NKDTKIAKK-Nle-A(562-572)-K(Palm)A to male rats on adenylate cyclase activity in the testicular membranes in vitro and on plasma testosterone levels. Peptide NKDTKIAKK-Nle-A(562-572)-K(Palm)A stimulated basal adenylate cyclase activity and reduced activity of the enzyme in testicular membranes stimulated by chorionic gonadotropin. After intratesticular administration in a dose of 200 μg/kg, it significantly increased testosterone so that 1, 3 and 5 h after administration its level was increased by 74, 44 and 35%, respectively. Administered intraperitoneally in a lower dose (50 μg/kg), the peptide had little effect on testosterone level. Unmodified peptide was inactive.
authors.affiliation: Laboratory for Molecular Endocrinology, I. M. Sechenov Institute of Evolutionary Physiology and Biochemistry, Russian Academy of Sciences, St. Petersburg, Russia.
authors.forename: K V
authors.initials: KV
authors.lastname: Derkach
authors.forename: E A
authors.initials: EA
authors.lastname: Shpakova
authors.forename: A O
authors.initials: AO
authors.lastname: Shpakov
mesh_terms: D000262:Adenylyl Cyclases / Q000378:metabolism; D000595:Amino Acid Sequence; D000704:Analysis of Variance; D000818:Animals; D007274:Injections, Intraperitoneal; D054878:Lipoylation / Q000235:genetics; D008297:Male; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000008:administration & dosage / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D011974:Receptors, LH / Q000235:genetics / Q000378:metabolism*; D013737:Testis / Q000378:metabolism; D013739:Testosterone / Q000096:biosynthesis* / Q000097:blood; D013997:Time Factors
chemical_list: D010446:Peptide Fragments; D011974:Receptors, LH; D013739:Testosterone; D000262:Adenylyl Cyclases