title: CXXC5 regulates differentiation of C2C12 myoblasts into myocytes.
abstract: CXXC5 is a member of the CXXC-type zinc-finger domain containing protein family, which is suggested to function in gene transcription, cell adhesion and cytoskeleton organization. Previous studies have revealed that CXXC5 is expressed in skeletal muscle, but whether it regulates skeletal myogenesis is yet unknown. Here, we screened for the possible signaling pathways in which CXXC5 might participate using luciferase gene reporters. The results indicated that CXXC5 significantly increased the activities of the promoters of genes involved in skeletal muscle differentiation. We therefore studied the role of CXXC5 during skeletal myogenesis in C2C12 myoblasts. Our findings suggest that overexpression of CXXC5 in C2C12 myoblasts facilitated myocyte differentiation, while RNAi interference of CXXC5 significantly inhibited the differentiation of C2C12 myoblasts. This study suggests that CXXC5 plays a significant role in regulating skeletal myogenesis.
authors.affiliation: The Center for Heart Development, Key Lab of MOE for Development Biology and Protein Chemistry, College of Life Sciences, Hunan Normal University, Changsha, 410081, Hunan, China.
authors.forename: Guangming
authors.initials: G
authors.lastname: Li
authors.forename: Xiangli
authors.initials: X
authors.lastname: Ye
authors.forename: Xiyang
authors.initials: X
authors.lastname: Peng
authors.forename: Yun
authors.initials: Y
authors.lastname: Deng
authors.forename: Wuzhou
authors.initials: W
authors.lastname: Yuan
authors.forename: Yongqing
authors.initials: Y
authors.lastname: Li
authors.forename: Xiaoyang
authors.initials: X
authors.lastname: Mo
authors.forename: Xijun
authors.initials: X
authors.lastname: Wang
authors.forename: Yongqi
authors.initials: Y
authors.lastname: Wan
authors.forename: Xianchu
authors.initials: X
authors.lastname: Liu
authors.forename: Tingfang
authors.initials: T
authors.lastname: Chen
authors.forename: Zhigang
authors.initials: Z
authors.lastname: Jiang
authors.forename: Xiongwei
authors.initials: X
authors.lastname: Fan
authors.forename: Xiushan
authors.initials: X
authors.lastname: Wu
authors.forename: Yuequn
authors.initials: Y
authors.lastname: Wang
mesh_terms: D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D002454:Cell Differentiation / Q000502:physiology; D002460:Cell Line; D004268:DNA-Binding Proteins; D057809:HEK293 Cells; D006801:Humans; D032342:Muscle Cells / Q000166:cytology* / Q000378:metabolism*; D032446:Myoblasts / Q000166:cytology* / Q000378:metabolism*; D015398:Signal Transduction; D014157:Transcription Factors
chemical_list: C540448:CXXC5 protein, human; D002352:Carrier Proteins; D004268:DNA-Binding Proteins; D014157:Transcription Factors