title: Transformation of cell-derived microparticles into quantum-dot-labeled nanovectors for antitumor siRNA delivery.
abstract: Cell-derived microparticles (MPs) have been recently recognized as critical intercellular information conveyors. However, further understanding of their biological behavior and potential application has been hampered by the limitations of current labeling techniques. Herein, a universal donor-cell-assisted membrane biotinylation strategy was proposed for labeling MPs by skillfully utilizing the natural membrane phospholipid exchange of their donor cells. This innovative strategy conveniently led to specific, efficient, reproducible, and biocompatible quantum dot (QD) labeling of MPs, thereby reliably conferring valuable traceability on MPs. By further loading with small interference RNA, QD-labeled MPs that had inherent cell-targeting and biomolecule-conveying ability were successfully employed for combined bioimaging and tumor-targeted therapy. This study provides the first reliable and biofriendly strategy for transforming biogenic MPs into functionalized nanovectors.
authors.affiliation: Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, and the Institute for Advanced Studies, Wuhan University, 299 Bayi Road, Wuhan 430072 (China); Key Laboratory of Oral Biomedicine (Ministry of Education) and Department of Oral and Maxillofacial Surgery, School and Hospital of Stomatology, Wuhan University, 237 Luoyu Road, Wuhan 430079 (China).
authors.forename: Gang
authors.initials: G
authors.lastname: Chen
authors.forename: Jun-Yi
authors.initials: JY
authors.lastname: Zhu
authors.forename: Zhi-Ling
authors.initials: ZL
authors.lastname: Zhang
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.forename: Jian-Gang
authors.initials: JG
authors.lastname: Ren
authors.forename: Min
authors.initials: M
authors.lastname: Wu
authors.forename: Zheng-Yuan
authors.initials: ZY
authors.lastname: Hong
authors.forename: Cheng
authors.initials: C
authors.lastname: Lv
authors.forename: Dai-Wen
authors.initials: DW
authors.lastname: Pang
authors.forename: Yi-Fang
authors.initials: YF
authors.lastname: Zhao
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry* / Q000378:metabolism / Q000627:therapeutic use; D017209:Apoptosis; D019921:Biotinylation; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D055252:Cell-Derived Microparticles / Q000737:chemistry*; D004337:Drug Carriers / Q000737:chemistry; D005452:Fluoresceins / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D008856:Microscopy, Fluorescence; D053758:Nanoparticles / Q000737:chemistry; D009369:Neoplasms / Q000175:diagnosis / Q000188:drug therapy; D045663:Quantum Dots / Q000737:chemistry*; D034741:RNA, Small Interfering / Q000737:chemistry* / Q000378:metabolism / Q000627:therapeutic use; D019809:Streptavidin / Q000737:chemistry; D013388:Succinimides / Q000737:chemistry; D014183:Transplantation, Heterologous; D042461:Vascular Endothelial Growth Factor A / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism
chemical_list: C087165:5-(6)-carboxyfluorescein diacetate succinimidyl ester; D000970:Antineoplastic Agents; D004337:Drug Carriers; D005452:Fluoresceins; D005456:Fluorescent Dyes; D034741:RNA, Small Interfering; D013388:Succinimides; D042461:Vascular Endothelial Growth Factor A; D019809:Streptavidin
keywords: biogenic vesicles; cell-derived microparticles; nanoparticles; quantum dots; targeted delivery