title: Studies of the dynamics of biological macromolecules using Au nanoparticle-DNA artificial molecules.
abstract: The recent development of graphene liquid cells, a nanoscale version of liquid bubble wrap, is a breakthrough for in situ liquid phase electron microscopy (EM). Using ultrathin graphene sheets as the liquid sample container, graphene liquid cells have allowed the unprecedented atomic resolution observation of solution phase growth and dynamics of nanocrystals. Here we explore the potential of this technique to probe nanoscale structure and dynamics of biomolecules in situ, using artificial Au nanoparticle-DNA artificial molecules as model systems. The interactions of electrons with both the artificial molecules and the liquid environment have been demonstrated and discussed, revealing both the opportunities and challenges of using graphene liquid cell EM as a new method of bio-imaging.
authors.affiliation: Department of Chemistry, University of California, Berkeley, CA 94720, USA. alivis@berkeley.edu.
authors.forename: Qian
authors.initials: Q
authors.lastname: Chen
authors.forename: Jessica M
authors.initials: JM
authors.lastname: Smith
authors.forename: Haider I
authors.initials: HI
authors.lastname: Rasool
authors.forename: Alex
authors.initials: A
authors.lastname: Zettl
authors.forename: A Paul
authors.initials: AP
authors.lastname: Alivisatos
mesh_terms: D004277:DNA, Single-Stranded / Q000737:chemistry*; D006046:Gold / Q000737:chemistry*; D006108:Graphite / Q000737:chemistry; D046911:Macromolecular Substances / Q000737:chemistry; D053768:Metal Nanoparticles / Q000737:chemistry*; D013816:Thermodynamics
chemical_list: D004277:DNA, Single-Stranded; D046911:Macromolecular Substances; D006046:Gold; D006108:Graphite