title: A piezoelectric immunosensor for early cervical cancer detection.
abstract: BACKGROUND
  A piezoelectric immunosensor for early cervical cancer detection was developed involving short analyis time and less invasive technique for p16INK4a, a protein that has been linked to cervical cancer.
  
  
  METHODS
  5μL of 5.0 mg/mL p16INK4a antibody and then supernatant from different clinical samples from West China Second University Hospital (Sichuan, China) were dripped on the center of the AT-cut crystal through a micro-injector. Absorption of the p16INK4a by antibody caused a shift in the resonant frequency of the immunosensor, and the resonant frequency was correlated to the amount of the p16INK4a in the supernatant.
  
  
  RESULTS
  The greater severity of lesion grading, the greater the expression level of p16INK4.
  
  
  CONCLUSIONS
  Degree of cervical cancer lesion development could be determined by detected amount of p16INK4a in different clinical samples.
authors.affiliation: Frequency Control Laboratory, School of Automation and Engineering, University of Electronic Science and Technology of China, Chengdu, Sichuan, China E-mail : swun_yangli@163.com.
authors.forename: Li
authors.initials: L
authors.lastname: Yang
authors.forename: Xianhe
authors.initials: X
authors.lastname: Huang
authors.forename: Liang
authors.initials: L
authors.lastname: Sun
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D002294:Carcinoma, Squamous Cell / Q000175:diagnosis*; D002681:China; D055088:Early Detection of Cancer / Q000379:methods*; D005260:Female; D006801:Humans; D040081:Protein Array Analysis / Q000379:methods; D011791:Quartz; D012494:Sampling Studies; D012680:Sensitivity and Specificity; D002583:Uterine Cervical Neoplasms / Q000175:diagnosis*
chemical_list: D011791:Quartz