title: miR-103/107 modulates multidrug resistance in human gastric carcinoma by downregulating Cav-1.
abstract: MicroRNAs (miRNAs) are a class of non-protein-coding small RNAs with the capacity to regulate fundamental biological processes essential for cancer initiation and progression. In the present study, we analyzed miRNA expression levels between multidrug-resistant gastric carcinoma cell line SGC7901/ADR and its parent cell line SGC7901 using a miRNA microarray. MiR-103/107 was downregulated compared with parental SGC7901 cells. Overexpression of miR-103/107 sensitized SGC7901/ADR cells to doxorubicin (DOX), as demonstrated by in vitro and in vivo drug sensitivity assay. We further confirmed that miR-103/107 inhibited P-gp function in gastric cancer SGC7901/ADR cells. Finally, we verified that caveolin-1 (Cav-1), a critical component of lipid rafts, was a target of miR-103/107.
authors.affiliation: Department of Medical Oncology, The First Hospital of China Medical University, NO. 155, North Nanjing Street, Heping District, Shenyang, 110001, China.
authors.forename: Ye
authors.initials: Y
authors.lastname: Zhang
authors.forename: Xiujuan
authors.initials: X
authors.lastname: Qu
authors.forename: Ce
authors.initials: C
authors.lastname: Li
authors.forename: Yibo
authors.initials: Y
authors.lastname: Fan
authors.forename: Xiaofang
authors.initials: X
authors.lastname: Che
authors.forename: Ximing
authors.initials: X
authors.lastname: Wang
authors.forename: Ying
authors.initials: Y
authors.lastname: Cai
authors.forename: Xuejun
authors.initials: X
authors.lastname: Hu
authors.forename: Yunpeng
authors.initials: Y
authors.lastname: Liu
mesh_terms: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000235:genetics; D017209:Apoptosis / Q000187:drug effects; D002277:Carcinoma / Q000188:drug therapy / Q000235:genetics* / Q000473:pathology; D051242:Caveolin 1 / Q000096:biosynthesis*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002945:Cisplatin / Q000008:administration & dosage; D004317:Doxorubicin / Q000008:administration & dosage; D018432:Drug Resistance, Multiple / Q000235:genetics; D019008:Drug Resistance, Neoplasm / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D013274:Stomach Neoplasms / Q000188:drug therapy / Q000235:genetics* / Q000473:pathology
chemical_list: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D051242:Caveolin 1; C544749:MIRN103 microRNA, human; D035683:MicroRNAs; D004317:Doxorubicin; D002945:Cisplatin