title: Production of thermostable hydrolases (cellulases and xylanase) from Thermoascus aurantiacus RCKK: a potential fungus.
abstract: Thermophilic fungi are potential sources of thermostable enzymes and other value added products. Present study has focused on optimization of different physicochemical parameters for production of thermostable cellulases and xylanase by Thermoascus aurantiacus RCKK under SSF. Enzyme production was supported maximally on wheat bran fed with 20% inoculum, at initial pH 5, temperature 45 °C and moisture ratio 1:3. The supplementation of wheat bran with yeast extract, Tween-80 and glycine further improved enzyme titres (CMCase 88 IU/g, FPase 15.8 IU/g, β-glucosidase 25.3 IU/g and xylanase 6,543 IU/g). The crude enzymes hydrolyzed phosphoric acid-swollen wheat straw, avicel and untreated xylan up to 74, 71 and 90%, respectively. In addition, T. aurantiacus RCKK produced antioxidants as fermentation by-products with significant %DPPH(∙) scavenging, FRAP and in vivo antioxidant capacity against H2O2-treated Saccharomyces cerevisiae. These capabilities show that it holds potential to exploit crop by-products for providing various commodities.
authors.affiliation: Department of Microbiology, Lignocellulose Biotechnology Laboratory, University of Delhi South Campus, Benito Juarez Road, New Delhi, 110021, India.
authors.forename: Kavish Kumar
authors.initials: KK
authors.lastname: Jain
authors.forename: Tapati
authors.initials: T
authors.lastname: Bhanja Dey
authors.forename: Sandeep
authors.initials: S
authors.lastname: Kumar
authors.forename: Ramesh Chander
authors.initials: RC
authors.lastname: Kuhad
mesh_terms: D000975:Antioxidants / Q000737:chemistry; D056804:Biofuels; D001709:Biotechnology; D002480:Cellulase / Q000096:biosynthesis*; D002855:Chromatography, Thin Layer; D043364:Endo-1,4-beta Xylanases / Q000096:biosynthesis*; D005285:Fermentation; D006861:Hydrogen Peroxide / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D010756:Phosphoric Acids / Q000737:chemistry; D010802:Phylogeny; D012441:Saccharomyces cerevisiae / Q000201:enzymology; D013696:Temperature; D055327:Thermoascus / Q000201:enzymology*; D014908:Triticum
chemical_list: D000975:Antioxidants; D056804:Biofuels; D010756:Phosphoric Acids; D006861:Hydrogen Peroxide; C030242:phosphoric acid; D002480:Cellulase; D043364:Endo-1,4-beta Xylanases