title: A comparative mechanical analysis of plant and animal cells reveals convergence across kingdoms.
abstract: Plant and animals have evolved different strategies for their development. Whether this is linked to major differences in their cell mechanics remains unclear, mainly because measurements on plant and animal cells relied on independent experiments and setups, thus hindering any direct comparison. In this study we used the same micro-rheometer to compare animal and plant single cell rheology. We found that wall-less plant cells exhibit the same weak power law rheology as animal cells, with comparable values of elastic and loss moduli. Remarkably, microtubules primarily contributed to the rheological behavior of wall-less plant cells whereas rheology of animal cells was mainly dependent on the actin network. Thus, plant and animal cells evolved different molecular strategies to reach a comparable cytoplasmic mechanical core, suggesting that evolutionary convergence could include the internal biophysical properties of cells.
authors.affiliation: Laboratoire Matière et Systèmes Complexes, UMR 7057 CNRS & Université Paris Diderot, Sorbonne Paris Cité, 10 rue Alice Domon et Léonie Duquet, 75013, Paris, France.
authors.forename: Pauline
authors.initials: P
authors.lastname: Durand-Smet
authors.affiliation: Laboratoire Matière et Systèmes Complexes, UMR 7057 CNRS & Université Paris Diderot, Sorbonne Paris Cité, 10 rue Alice Domon et Léonie Duquet, 75013, Paris, France.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Chastrette
authors.affiliation: Laboratoire Matière et Systèmes Complexes, UMR 7057 CNRS & Université Paris Diderot, Sorbonne Paris Cité, 10 rue Alice Domon et Léonie Duquet, 75013, Paris, France.
authors.forename: Axel
authors.initials: A
authors.lastname: Guiroy
authors.affiliation: Laboratoire Matière et Systèmes Complexes, UMR 7057 CNRS & Université Paris Diderot, Sorbonne Paris Cité, 10 rue Alice Domon et Léonie Duquet, 75013, Paris, France.
authors.forename: Alain
authors.initials: A
authors.lastname: Richert
authors.affiliation: Laboratoire de Reproduction de développement des plantes, INRA, CNRS, ENS Lyon, UCB Lyon 1, Université de Lyon, 46 Allée d'Italie, 69364 Lyon, France.
authors.forename: Annick
authors.initials: A
authors.lastname: Berne-Dedieu
authors.affiliation: Laboratoire de Reproduction de développement des plantes, INRA, CNRS, ENS Lyon, UCB Lyon 1, Université de Lyon, 46 Allée d'Italie, 69364 Lyon, France.
authors.forename: Judit
authors.initials: J
authors.lastname: Szecsi
authors.affiliation: Laboratoire de Reproduction de développement des plantes, INRA, CNRS, ENS Lyon, UCB Lyon 1, Université de Lyon, 46 Allée d'Italie, 69364 Lyon, France; Laboratoire Joliot Curie, CNRS, ENS Lyon, Université de Lyon, 46 Allée d'Italie, 69364 Lyon, France.
authors.forename: Arezki
authors.initials: A
authors.lastname: Boudaoud
authors.affiliation: Institut des Sciences du Végétal, CNRS, Saclay Plant Sciences, bat 23 - Avenue de la Terrasse - 91198 Gif-sur-Yvette, France.
authors.forename: Jean-Marie
authors.initials: JM
authors.lastname: Frachisse
authors.forename: Mohammed
authors.initials: M
authors.lastname: Bendahmane
authors.affiliation: Laboratoire de Reproduction de développement des plantes, INRA, CNRS, ENS Lyon, UCB Lyon 1, Université de Lyon, 46 Allée d'Italie, 69364 Lyon, France.
authors.forename: Mohammed
authors.initials: M
authors.lastname: Bendhamane
authors.affiliation: Laboratoire de Reproduction de développement des plantes, INRA, CNRS, ENS Lyon, UCB Lyon 1, Université de Lyon, 46 Allée d'Italie, 69364 Lyon, France; Laboratoire Joliot Curie, CNRS, ENS Lyon, Université de Lyon, 46 Allée d'Italie, 69364 Lyon, France.
authors.forename: Oliver
authors.initials: O
authors.lastname: Hamant
authors.affiliation: Laboratoire Matière et Systèmes Complexes, UMR 7057 CNRS & Université Paris Diderot, Sorbonne Paris Cité, 10 rue Alice Domon et Léonie Duquet, 75013, Paris, France. Electronic address: atef.asnacios@univ-paris-diderot.fr.
authors.forename: Atef
authors.initials: A
authors.lastname: Asnacios
mesh_terms: D000818:Animals; D017360:Arabidopsis / Q000166:cytology*; D001696:Biomechanical Phenomena; D002460:Cell Line; D055595:Mechanical Phenomena; D051379:Mice; D008870:Microtubules / Q000378:metabolism; D059010:Single-Cell Analysis; D013045:Species Specificity