title: High-throughput synthesis of stable isotope-labeled transmembrane proteins for targeted transmembrane proteomics using a wheat germ cell-free protein synthesis system.
abstract: Using a wheat germ cell-free protein synthesis system, we developed a high-throughput method for the synthesis of stable isotope-labeled full-length transmembrane proteins as proteoliposomes to mimic the in vivo environment, and we successfully constructed an internal standard library for targeted transmembrane proteomics by using mass spectrometry.
authors.affiliation: Proteo-Science Center, Ehime University, Ehime, Japan. takemori@m.ehime-u.ac.jp.
authors.forename: Nobuaki
authors.initials: N
authors.lastname: Takemori
authors.forename: Ayako
authors.initials: A
authors.lastname: Takemori
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Matsuoka
authors.forename: Ryo
authors.initials: R
authors.lastname: Morishita
authors.forename: Natsuki
authors.initials: N
authors.lastname: Matsushita
authors.forename: Masato
authors.initials: M
authors.lastname: Aoshima
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Takeda
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Sawasaki
authors.forename: Yaeta
authors.initials: Y
authors.lastname: Endo
authors.forename: Shigeki
authors.initials: S
authors.lastname: Higashiyama
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002474:Cell-Free System; D007553:Isotope Labeling / Q000379:methods*; D008565:Membrane Proteins / Q000737:chemistry / Q000378:metabolism*; D051379:Mice; D008969:Molecular Sequence Data; D014176:Protein Biosynthesis; D040901:Proteomics / Q000379:methods*; D017981:Receptors, Neurotransmitter / Q000378:metabolism; D014908:Triticum / Q000737:chemistry*
chemical_list: D008565:Membrane Proteins; D017981:Receptors, Neurotransmitter