title: Studies on fish scale collagen of Pacific saury (Cololabis saira).
abstract: We purified and characterized Type I collagen from the scales of the Pacific saury (Cololabis saira) and compared it with collagen from other organisms. Subunit composition of C. saira collagen (2α1+α2) was similar to that of red sea bream (Pagrus major) and porcine collagen. C. saira collagen did not form a firm gel after neutralization of pH in solution. The temperature of denaturation (24-25 °C) of C. saira collagen was slightly lower than that of P. major collagen (26-27 °C). The contents of proline and hydroxyproline were lower in red sea bream and Pacific saury collagen than in porcine collagen. Circular dichroism spectra and Fourier-transformed infrared spectra showed that heat denaturation caused unfolding of the triple helices in all three collagens.
authors.affiliation: Department of Biological Science, Graduate School of Science, Osaka Prefecture University, 1-2 Gakuen-cho, Naka-ku, Sakai, Osaka 599-8570, Japan.
authors.forename: Hideki
authors.initials: H
authors.lastname: Mori
authors.affiliation: Department of Biological Science, Graduate School of Science, Osaka Prefecture University, 1-2 Gakuen-cho, Naka-ku, Sakai, Osaka 599-8570, Japan.
authors.forename: Yurie
authors.initials: Y
authors.lastname: Tone
authors.affiliation: Department of Biological Science, Graduate School of Science, Osaka Prefecture University, 1-2 Gakuen-cho, Naka-ku, Sakai, Osaka 599-8570, Japan.
authors.forename: Kouske
authors.initials: K
authors.lastname: Shimizu
authors.affiliation: Department of Biological Science, Graduate School of Science, Osaka Prefecture University, 1-2 Gakuen-cho, Naka-ku, Sakai, Osaka 599-8570, Japan.
authors.forename: Kazunori
authors.initials: K
authors.lastname: Zikihara
authors.affiliation: Department of Biological Science, Graduate School of Science, Osaka Prefecture University, 1-2 Gakuen-cho, Naka-ku, Sakai, Osaka 599-8570, Japan.
authors.forename: Satoru
authors.initials: S
authors.lastname: Tokutomi
authors.affiliation: Department of Biological Science, Graduate School of Science, Osaka Prefecture University, 1-2 Gakuen-cho, Naka-ku, Sakai, Osaka 599-8570, Japan.
authors.forename: Tomoaki
authors.initials: T
authors.lastname: Ida
authors.affiliation: Department of Biological Science, Graduate School of Science, Osaka Prefecture University, 1-2 Gakuen-cho, Naka-ku, Sakai, Osaka 599-8570, Japan.
authors.forename: Hideshi
authors.initials: H
authors.lastname: Ihara
authors.affiliation: Department of Biological Science, Graduate School of Science, Osaka Prefecture University, 1-2 Gakuen-cho, Naka-ku, Sakai, Osaka 599-8570, Japan. Electronic address: hara@b.s.osakafu-u.ac.jp.
authors.forename: Masayuki
authors.initials: M
authors.lastname: Hara
mesh_terms: D000818:Animals; D023761:Beloniformes / Q000378:metabolism*; D002942:Circular Dichroism; D003094:Collagen / Q000737:chemistry*; D005782:Gels / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D006909:Hydroxyproline / Q000737:chemistry; D011392:Proline / Q000737:chemistry; D011489:Protein Denaturation; D055550:Protein Stability; D017433:Protein Structure, Secondary; D021541:Sea Bream / Q000378:metabolism; D017550:Spectroscopy, Fourier Transform Infrared; D013552:Swine; D044366:Transition Temperature
chemical_list: D005782:Gels; D003094:Collagen; D011392:Proline; D006909:Hydroxyproline