title: Transcriptome profiling identifies differentially expressed genes in Huoyan goose ovaries between the laying period and ceased period.
abstract: The Huoyan goose is famous for its high egg-laying performance and is listed as a nationally protected domestic animal by the Chinese government. To elucidate the key regulatory genes involved in Huoyan goose egg laying, RNA from ovarian tissue during the ceased and laying periods was sequenced using the Illumina HiSeq 2000 sequencing platform. More than 12 million reads were produced in ceased and laying libraries that included 11,896,423 and 12,534,799 clean reads, respectively. More than 20% of the reads were matched to the reference genome, and 23% of the reads were matched to reference genes. Genes with a false discovery rate (FDR) ≤0.001 and log2ratio ≧1 or ≤-1 were characterized as differentially expressed, and 344 up-regulated and 344 down-regulated genes were classified into functional categories. Twelve genes that are mainly involved in pathways for reproduction regulation, such as steroid hormone biosynthesis, GnRH signaling pathways, oocyte meiosis, progesterone-mediated oocyte maturation, steroid biosynthesis, calcium signaling pathways, and G-protein coupled receptor signaling pathway were selected for validation by a quantitative real-time polymerase chain reaction (qRT-PCR) analysis, the qRT-PCR results are consistent with the general expression patterns of those genes from the Illumina sequencing. These data provide comprehensive gene expression information at the transcriptional level that might increase our understanding of the Huoyan goose's reproductive biology.
authors.affiliation: College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang, 110866, China.
authors.forename: Xinhong
authors.initials: X
authors.lastname: Luan
authors.affiliation: College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang, 110866, China.
authors.forename: Dawei
authors.initials: D
authors.lastname: Liu
authors.affiliation: College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang, 110866, China.
authors.forename: Zhongzan
authors.initials: Z
authors.lastname: Cao
authors.affiliation: College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang, 110866, China.
authors.forename: Lina
authors.initials: L
authors.lastname: Luo
authors.affiliation: College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang, 110866, China.
authors.forename: Mei
authors.initials: M
authors.lastname: Liu
authors.affiliation: College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang, 110866, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Gao
authors.affiliation: Liaoning Province Livestock and Poultry Genetic Resources Conservation and Utilization Center, Liaoyang, 111000, China.
authors.forename: Xiaoying
authors.initials: X
authors.lastname: Zhang
mesh_terms: D000818:Animals; D000858:Anovulation / Q000235:genetics; D030161:Avian Proteins / Q000235:genetics; D005260:Female; D005777:Geese / Q000235:genetics*; D020869:Gene Expression Profiling; D015723:Gene Library; D063990:Gene Ontology; D010053:Ovary / Q000378:metabolism*; D010060:Ovulation / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017423:Sequence Analysis, RNA; D059467:Transcriptome
chemical_list: D030161:Avian Proteins