title: Fast determination of virgin olive oil phenolic metabolites in human high-density lipoproteins.
abstract: In recent years it has been confirmed that the consumption of olive oil prevents the oxidation of biomolecules owing to its monounsaturated fatty acids (MUFA) and phenolic content. The main objective of the study was to develop an ultra-high-performance liquid chromatography (UHPLC) tandem mass spectrometry (MS/MS) method for the determination of phenolic compounds in human high-density lipoprotein (HDL) samples. At the same time, the influence of olive oil consumption on the phenolic metabolite levels was evaluated in a European population. The participants were 51 healthy men, aged 20-60. They were randomized to two consecutive intervention periods with the administration of raw olive oil with low and high polyphenolic content. The UHPLC-MS/MS analytical method has been validated for hydroxytyrosol and homovanillic acid in terms of linearity (r(2)  = 0.99 and 1.00), repeatability (5.7 and 6.5%) reproducibility (6.2 and 7%), recovery (98 to 97%), limits of detection (1.7 to 1.8 ppb) and quantification (5.8 and 6.3 ppb).The levels of the studied metabolites increased significantly after high polyphenolic content virgin olive oil ingestion (p <0.05) compared with lowpolyphenolic content olive oil. Virgin olive oil consumption increases the levels of phenolic metabolites in HDL and thus provides human HDL with more efficient antioxidant protection.
authors.affiliation: Department of Nutrition and Food Science, Faculty of Pharmacy, University of Barcelona, Barcelona, Spain.
authors.forename: C
authors.initials: C
authors.lastname: Fernández-Ávila
authors.affiliation: Department of Nutrition and Food Science, Faculty of Pharmacy, University of Barcelona, Barcelona, Spain.|CIBER Fisiopatología de la Obesidad y Nutrición, Madrid, Spain.
authors.forename: R
authors.identifier: http://orcid.org/0000-0002-4154-3541
authors.initials: R
authors.lastname: Montes
authors.affiliation: Department of Nutrition and Food Science, Faculty of Pharmacy, University of Barcelona, Barcelona, Spain.|CIBER Fisiopatología de la Obesidad y Nutrición, Madrid, Spain.
authors.forename: A I
authors.initials: AI
authors.lastname: Castellote
authors.affiliation: Department of Nutrition and Food Science, Faculty of Pharmacy, University of Barcelona, Barcelona, Spain.|CIBER Fisiopatología de la Obesidad y Nutrición, Madrid, Spain.
authors.forename: A M
authors.initials: AM
authors.lastname: Chisaguano
authors.affiliation: Lipids and Cardiovascular Epidemiology Unit, Institut Municipal d'Investigació Mèdica (IMIM), Barcelona, Spain.
authors.forename: M
authors.initials: M
authors.lastname: Fitó
authors.affiliation: Lipids and Cardiovascular Epidemiology Unit, Institut Municipal d'Investigació Mèdica (IMIM), Barcelona, Spain.
authors.forename: M I
authors.initials: MI
authors.lastname: Covas
authors.affiliation: Lipids and Cardiovascular Epidemiology Unit, Institut Municipal d'Investigació Mèdica (IMIM), Barcelona, Spain.
authors.forename: D
authors.initials: D
authors.lastname: Muñoz-Aguallo
authors.affiliation: Research Institute of Public Health, University of Kuopio, Kuopio, Finland.
authors.forename: K
authors.initials: K
authors.lastname: Nyyssönen
authors.affiliation: German Institute of Human Nutrition, Postdam-Rehbruecke, Germany.
authors.forename: H J
authors.initials: HJ
authors.lastname: Zunft
authors.affiliation: Department of Nutrition and Food Science, Faculty of Pharmacy, University of Barcelona, Barcelona, Spain.|CIBER Fisiopatología de la Obesidad y Nutrición, Madrid, Spain.
authors.forename: M C
authors.initials: MC
authors.lastname: López-Sabater
mesh_terms: D000328:Adult; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006801:Humans; D057230:Limit of Detection; D016014:Linear Models; D008075:Lipoproteins, HDL / Q000097:blood* / Q000737:chemistry*; D008297:Male; D008875:Middle Aged; D000069463:Olive Oil / Q000737:chemistry*; D010636:Phenols / Q000032:analysis*; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry / Q000379:methods; D055815:Young Adult
chemical_list: D008075:Lipoproteins, HDL; D000069463:Olive Oil; D010636:Phenols
keywords: high density lipoproteins; olive oil; phenolic content; tandem mass spectrometry; ultra-high performance liquid chromatography