title: Rapidly-deposited polydopamine coating via high temperature and vigorous stirring: formation, characterization and biofunctional evaluation.
abstract: Polydopamine (PDA) coating provides a promising approach for immobilization of biomolecules onto almost all kinds of solid substrates. However, the deposition kinetics of PDA coating as a function of temperature and reaction method is not well elucidated. Since dopamine self-polymerization usually takes a long time, therefore, rapid-formation of PDA film becomes imperative for surface modification of biomaterials and medical devices. In the present study, a practical method for preparation of rapidly-deposited PDA coating was developed using a uniquely designed device, and the kinetics of dopamine self-polymerization was investigated by QCM sensor system. It was found that high temperature and vigorous stirring could dramatically speed up the formation of PDA film on QCM chip surface. Surface characterization, BSA binding study, cell viability assay and antibacterial test demonstrates that the polydopamine coating after polymerization for 30 min by our approach exhibits similar properties to those of 24 h counterpart. The method has a great potential for rapid-deposition of polydopamine films to modify biomaterial surfaces.
authors.affiliation: Department of Oral and Maxillofacial Surgery, School and Hospital of Stomatology, Peking University, Beijing, China; Center for Biomedical Materials and Tissue Engineering, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Zhou
authors.affiliation: Department of Oral and Maxillofacial Surgery, School and Hospital of Stomatology, Peking University, Beijing, China; Center for Biomedical Materials and Tissue Engineering, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Deng
authors.affiliation: Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou, China.
authors.forename: Beier
authors.initials: B
authors.lastname: Lyu
authors.affiliation: Department of Stomatology, Beijing Anzhen Hospital, Capital Medical University, Beijing, China.
authors.forename: Ranran
authors.initials: R
authors.lastname: Zhang
authors.affiliation: Department of Stomatology, Beijing Anzhen Hospital, Capital Medical University, Beijing, China; Department of Restorative Dentistry, School of Dentistry, University of Washington, Washington, United States of America.
authors.forename: Hai
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou, China.
authors.forename: Hongwei
authors.initials: H
authors.lastname: Ma
authors.affiliation: Department of Stomatology, Beijing Anzhen Hospital, Capital Medical University, Beijing, China.
authors.forename: Yalin
authors.initials: Y
authors.lastname: Lyu
authors.affiliation: Department of Oral and Maxillofacial Surgery, School and Hospital of Stomatology, Peking University, Beijing, China; Center for Biomedical Materials and Tissue Engineering, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing, China.
authors.forename: Shicheng
authors.initials: S
authors.lastname: Wei
mesh_terms: D000327:Adsorption; D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology; D001422:Bacterial Adhesion / Q000187:drug effects; D015374:Biosensing Techniques / Q000379:methods*; D002417:Cattle; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D004926:Escherichia coli / Q000502:physiology; D006358:Hot Temperature; D006801:Humans; D007211:Indoles / Q000737:chemistry* / Q000494:pharmacology; D007700:Kinetics; D008567:Membranes, Artificial; D018625:Microscopy, Atomic Force; D056951:Photoelectron Spectroscopy; D011108:Polymers / Q000737:chemistry* / Q000494:pharmacology; D058525:Quartz Crystal Microbalance Techniques / Q000379:methods*; D012710:Serum Albumin, Bovine / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared; D013295:Streptococcus mutans / Q000502:physiology; D013499:Surface Properties; D013997:Time Factors; D014465:Ultrasonics
chemical_list: D000900:Anti-Bacterial Agents; D007211:Indoles; D008567:Membranes, Artificial; D011108:Polymers; C568283:polydopamine; D012710:Serum Albumin, Bovine