title: A marine inducible prophage vB_CibM-P1 isolated from the aerobic anoxygenic phototrophic bacterium Citromicrobium bathyomarinum JL354.
abstract: A prophage vB_CibM-P1 was induced by mitomycin C from the epipelagic strain Citromicrobium bathyomarinum JL354, a member of the alpha-IV subcluster of marine aerobic anoxygenic phototrophic bacteria (AAPB). The induced bacteriophage vB_CibM-P1 had Myoviridae-like morphology and polyhedral heads (approximately capsid 60-100 nm) with tail fibers. The vB_CibM-P1 genome is ~38 kb in size, with 66.0% GC content. The genome contains 58 proposed open reading frames that are involved in integration, DNA packaging, morphogenesis and bacterial lysis. VB_CibM-P1 is a temperate phage that can be directly induced in hosts. In response to mitomycin C induction, virus-like particles can increase to 7 × 10(9) per ml, while host cells decrease an order of magnitude. The vB_CibM-P1 bacteriophage is the first inducible prophage from AAPB.
authors.affiliation: State Key Laboratory of Marine Environmental Science, Xiamen University, Xiamen 361005, P.R. China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Zheng
authors.affiliation: State Key Laboratory of Marine Environmental Science, Xiamen University, Xiamen 361005, P.R. China.
authors.forename: Rui
authors.initials: R
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Marine Environmental Science, Xiamen University, Xiamen 361005, P.R. China.
authors.forename: Yongle
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Department of Microbiology and Immunology, University of British Columbia, Vancouver, B.C., Canada V6N 1Z4.
authors.forename: Richard Allen
authors.initials: RA
authors.lastname: White
authors.affiliation: State Key Laboratory of Marine Environmental Science, Xiamen University, Xiamen 361005, P.R. China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Marine Environmental Science, Xiamen University, Xiamen 361005, P.R. China.
authors.forename: Tingwei
authors.initials: T
authors.lastname: Luo
authors.affiliation: State Key Laboratory of Marine Environmental Science, Xiamen University, Xiamen 361005, P.R. China.
authors.forename: Nianzhi
authors.initials: N
authors.lastname: Jiao
mesh_terms: D000332:Aerobiosis / Q000502:physiology; D020561:Alphaproteobacteria / Q000821:virology*; D059001:Aquatic Organisms; D001482:Base Composition; D042003:DNA Packaging / Q000502:physiology; D059646:Genome Size; D016679:Genome, Viral; D008242:Lysogeny / Q000502:physiology; D016685:Mitomycin / Q000494:pharmacology; D058977:Molecular Sequence Annotation; D017900:Myoviridae / Q000145:classification / Q000187:drug effects / Q000235:genetics* / Q000648:ultrastructure; D016366:Open Reading Frames; D052817:Phototrophic Processes / Q000502:physiology; D010802:Phylogeny; D039002:Prophages / Q000145:classification / Q000187:drug effects / Q000235:genetics* / Q000648:ultrastructure; D014764:Viral Proteins / Q000235:genetics*; D014771:Virion / Q000502:physiology; D014775:Virus Activation / Q000187:drug effects; D016662:Virus Integration / Q000502:physiology
chemical_list: D014764:Viral Proteins; D016685:Mitomycin