title: Affinity-based methods in drug-target discovery.
abstract: Target discovery using the molecular approach, as opposed to the more traditional systems approach requires the study of the cellular or biological process underlying a condition or disease. The approaches that are employed by the "bench" scientist may be genetic, genomic or proteomic and each has its rightful place in the drug-target discovery process. Affinity-based proteomic techniques currently used in drug-discovery draw upon several disciplines, synthetic chemistry, cell-biology, biochemistry and mass spectrometry. An important component of such techniques is the probe that is specifically designed to pick out a protein or set of proteins from amongst the varied thousands in a cell lysate. A second component, that is just as important, is liquid-chromatography tandem massspectrometry (LC-MS/MS). LC-MS/MS and the supporting theoretical framework has come of age and is the tool of choice for protein identification and quantification. These proteomic tools are critical to maintaining the drug-candidate supply, in the larger context of drug discovery.
authors.forename: Gabriela
authors.initials: G
authors.lastname: Rylova
authors.forename: Tomas
authors.initials: T
authors.lastname: Ozdian
authors.forename: Lakshman
authors.initials: L
authors.lastname: Varanasi
authors.forename: Miroslav
authors.initials: M
authors.lastname: Soural
authors.forename: Jan
authors.initials: J
authors.lastname: Hlavac
authors.forename: Dusan
authors.initials: D
authors.lastname: Holub
authors.forename: Petr
authors.initials: P
authors.lastname: Dzubak
authors.affiliation: Institute of Molecular and Translational Medicine Faculty of Medicine and Dentistry Palacky University in Olomouc, Hnevotinska 5, 779 00 Olomouc, Czech Republic. marian.hajduch@upol.cz.
authors.forename: Marian
authors.initials: M
authors.lastname: Hajduch
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods; D055808:Drug Discovery / Q000379:methods*; D006801:Humans; D011506:Proteins / Q000737:chemistry / Q000378:metabolism; D040901:Proteomics / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods
chemical_list: D011506:Proteins