title: Silver sulfadiazine loaded chitosan/chondroitin sulfate films for a potential wound dressing application.
abstract: Silver sulfadiazine (AgSD) loaded chitosan/chondroitin sulfate (CHI/CS) films were formed to be applied as a potential wound dressing material. The liquid uptake capacity of both, CHI/CS and CHI/CS/AgSD, films exhibited a pH-dependent behavior. Tensile tests showed that the amount of CS used to form the films and the further incorporation of AgSD affect the mechanical properties of the films. In vitro AgSD-release assays showed that the CHI/CS mass ratio influences the AgSD release rate. All the investigated CHI/CS/AgSD films sustain the AgSD release up to 96h at physiological pH. Antibacterial activity and cell viability assays showed that all the CHI/CS/AgSD films have activity against Pseudomonas aeruginosa and Staphylococcus aureus but they were not toxic to Vero cells. The results presented in this work indicate that the CHI/CS/AgSD exhibits potential to be applied as a wound dressing material.
authors.affiliation: Grupo de Materiais Poliméricos e Compósitos (GMPC), Department of Chemistry, Maringá State University, Av. Colombo, 5790, 87020-900, Maringá, Paraná, Brazil. Electronic address: drefajardo@hotmail.com.
authors.forename: André R
authors.initials: AR
authors.lastname: Fajardo
authors.affiliation: Grupo de Materiais Poliméricos e Compósitos (GMPC), Department of Chemistry, Maringá State University, Av. Colombo, 5790, 87020-900, Maringá, Paraná, Brazil.
authors.forename: Laís C
authors.initials: LC
authors.lastname: Lopes
authors.affiliation: Laboratório de Microbiologia Aplicada aos Produtos Naturais e Sintéticos, Department of Clinical Analysis, Maringá State University, Av. Colombo, 5790, 87020-900, Maringá, Paraná, Brazil; Laboratório de Inovação Tecnológica no Desenvolvimento de Fármacos e Cosméticos, Department of Clinical Analysis, Maringá State University, Av. Colombo, 5790, 87020-900, Maringá, Paraná, Brazil.
authors.forename: Angelo O
authors.initials: AO
authors.lastname: Caleare
authors.affiliation: Laboratório de Microbiologia Aplicada aos Produtos Naturais e Sintéticos, Department of Clinical Analysis, Maringá State University, Av. Colombo, 5790, 87020-900, Maringá, Paraná, Brazil; Laboratório de Inovação Tecnológica no Desenvolvimento de Fármacos e Cosméticos, Department of Clinical Analysis, Maringá State University, Av. Colombo, 5790, 87020-900, Maringá, Paraná, Brazil.
authors.forename: Elizandra A
authors.initials: EA
authors.lastname: Britta
authors.affiliation: Laboratório de Microbiologia Aplicada aos Produtos Naturais e Sintéticos, Department of Clinical Analysis, Maringá State University, Av. Colombo, 5790, 87020-900, Maringá, Paraná, Brazil; Laboratório de Inovação Tecnológica no Desenvolvimento de Fármacos e Cosméticos, Department of Clinical Analysis, Maringá State University, Av. Colombo, 5790, 87020-900, Maringá, Paraná, Brazil.
authors.forename: Celso V
authors.initials: CV
authors.lastname: Nakamura
authors.affiliation: Grupo de Materiais Poliméricos e Compósitos (GMPC), Department of Chemistry, Maringá State University, Av. Colombo, 5790, 87020-900, Maringá, Paraná, Brazil.
authors.forename: Adley F
authors.initials: AF
authors.lastname: Rubira
authors.affiliation: Grupo de Materiais Poliméricos e Compósitos (GMPC), Department of Chemistry, Maringá State University, Av. Colombo, 5790, 87020-900, Maringá, Paraná, Brazil.
authors.forename: Edvani C
authors.initials: EC
authors.lastname: Muniz
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000494:pharmacology / Q000633:toxicity; D001458:Bandages; D001672:Biocompatible Materials / Q000737:chemistry* / Q000494:pharmacology / Q000633:toxicity; D002470:Cell Survival / Q000187:drug effects; D048271:Chitosan / Q000737:chemistry* / Q000494:pharmacology / Q000633:toxicity; D002522:Chlorocebus aethiops; D002809:Chondroitin Sulfates / Q000737:chemistry* / Q000494:pharmacology / Q000633:toxicity; D011550:Pseudomonas aeruginosa / Q000187:drug effects; D012837:Silver Sulfadiazine / Q000737:chemistry* / Q000494:pharmacology / Q000633:toxicity; D013211:Staphylococcus aureus / Q000187:drug effects; D014709:Vero Cells
chemical_list: D000900:Anti-Bacterial Agents; D001672:Biocompatible Materials; C480355:chitosan sulfate; D002809:Chondroitin Sulfates; D048271:Chitosan; D012837:Silver Sulfadiazine