title: Changes in the proteomic profile during the differential polarization status of the human monocyte-derived macrophage THP-1 cell line.
abstract: Macrophages are heterogeneous and plastic populations that are an essential component of inflammation and host defense. To understand how macrophages respond to cytokine signals, we used 2DE to identify protein profiles in macrophages stimulated with interleukin 4 (M2) and those stimulated with lipopolysaccharide and interferon γ (M1). In total, 32 differentially expressed proteins in THP-1 cells were identified by MALDI-TOF MS/MS analysis. The different proteins were mainly involved in cellular structure, protein metabolism, stress response, oxidative response, and nitric oxide production during macrophage polarization. In particular, proteins playing important roles in production of nitric oxide (NO) were downregulated in M2 macrophages. Many antioxidant and heat shock proteins, which are related to oxidative response, were upregulated in M2 macrophages. More importantly, a remarkable decrease in intracellular ROS and NO production were detected in M2 macrophages. Our results provide a proteomic profile of differentially polarized macrophages and validate the function of the identified proteins, which may indicate possible mechanism of macrophage polarization process.
authors.affiliation: Department of Microbial and Biochemical Pharmacy, School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou, P. R. China.
authors.forename: Fan
authors.initials: F
authors.lastname: Zhang
authors.forename: Hao
authors.initials: H
authors.lastname: Liu
authors.forename: Guanmin
authors.initials: G
authors.lastname: Jiang
authors.forename: Hongsheng
authors.initials: H
authors.lastname: Wang
authors.forename: Xianfeng
authors.initials: X
authors.lastname: Wang
authors.forename: Hao
authors.initials: H
authors.lastname: Wang
authors.forename: Rui
authors.initials: R
authors.lastname: Fang
authors.forename: Shaohui
authors.initials: S
authors.lastname: Cai
authors.forename: Jun
authors.initials: J
authors.lastname: Du
mesh_terms: D002460:Cell Line; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D008264:Macrophages / Q000276:immunology* / Q000502:physiology*; D018448:Models, Immunological; D010641:Phenotype; D020543:Proteome / Q000032:analysis* / Q000737:chemistry; D040901:Proteomics
chemical_list: D020543:Proteome
keywords: 2DE; Cell biology; Inflammation; Macrophage polarization; Proteomic analysis; THP-1 cell