title: Wound healing potential of scaffolds prepared from porcine jejunum and urinary bladder by a non-detergent/enzymatic method.
abstract: Scaffolds prepared using extracellular matrices of mammalian organs/tissues, when used as grafts, have wound healing potential. This paper evaluated the physical properties and in vivo wound healing potential of jejunum-derived scaffold (JDS) and urinary bladder-derived scaffold (UDS) of porcine origin prepared by a non-detergent/enzymatic method. The former had higher flexural rigidity and suture retention strength compared to the latter, but both of them had the essential flexural rigidity and suture retention strength required for skin grafts. Full thickness skin-wounds on rabbit dorsum were treated with these scaffolds and the wound healing ability was compared by studying histomorphology parameters such as re-epithelialisation, collagen deposition, angiogenesis, proliferation of cells, mesenchymal cell infiltration and myofibroblast response. The extent of these reactions was assessed using histomorphometry. The results indicated that both grafts initiated healing faster than those wounds without any graft, as evidenced by the extent of cell proliferation and mesenchymal cell infiltration. The myofibroblast response persisted longer in the non-graft assisted wound healing reaction compared to the healing in the graft assisted wounds. Moreover, the JDS induced higher cell proliferation and greater angiogenesis than UDS probably indicating better healing by the former. The results suggested that JDS and UDS prepared by non-detergent/enzymatic method have potential clinical applications.
authors.affiliation: Division of Experimental Pathology, Biomedical Technology Wing, Sree Chitra Tirunal Institute for Medical Sciences and Technology, Trivandrum, India.
authors.forename: Deepa
authors.initials: D
authors.lastname: Revi
authors.affiliation: Division of Experimental Pathology, Biomedical Technology Wing, Sree Chitra Tirunal Institute for Medical Sciences and Technology, Trivandrum, India.
authors.forename: Vadavanath P
authors.initials: VP
authors.lastname: Vineetha
authors.affiliation: Division of Experimental Pathology, Biomedical Technology Wing, Sree Chitra Tirunal Institute for Medical Sciences and Technology, Trivandrum, India.
authors.forename: Jaseer
authors.initials: J
authors.lastname: Muhamed
authors.affiliation: Division of Experimental Pathology, Biomedical Technology Wing, Sree Chitra Tirunal Institute for Medical Sciences and Technology, Trivandrum, India.
authors.forename: Geetha C
authors.initials: GC
authors.lastname: Surendran
authors.affiliation: Division of Experimental Pathology, Biomedical Technology Wing, Sree Chitra Tirunal Institute for Medical Sciences and Technology, Trivandrum, India.
authors.forename: Akhila
authors.initials: A
authors.lastname: Rajan
authors.affiliation: Tissue Culture Laboratory, Biomedical Technology Wing, Sree Chitra Tirunal Institute for Medical Sciences and Technology, Trivandrum, India.
authors.forename: T V
authors.initials: TV
authors.lastname: Kumary
authors.affiliation: Division of Experimental Pathology, Biomedical Technology Wing, Sree Chitra Tirunal Institute for Medical Sciences and Technology, Trivandrum, India tvanilkumar@sctimst.ac.in.
authors.forename: Thapasimuthu V
authors.initials: TV
authors.lastname: Anilkumar
mesh_terms: D000818:Animals; D049109:Cell Proliferation; D003094:Collagen / Q000378:metabolism; D005109:Extracellular Matrix / Q000737:chemistry; D007583:Jejunum / Q000737:chemistry; D008422:Materials Testing; D059630:Mesenchymal Stem Cells / Q000473:pathology; D058628:Myofibroblasts / Q000473:pathology; D018919:Neovascularization, Physiologic; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D011817:Rabbits; D012867:Skin / Q000293:injuries* / Q000473:pathology / Q000503:physiopathology; D013552:Swine; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry; D001743:Urinary Bladder / Q000737:chemistry; D014945:Wound Healing
chemical_list: D018809:Proliferating Cell Nuclear Antigen; D003094:Collagen
keywords: Extracellular matrix; alpha smooth muscle actin; histomorphometry; proliferating cell nuclear antigen; wound healing