title: Lymph node stromal cells constrain immunity via MHC class II self-antigen presentation.
abstract: Non-hematopoietic lymph node stromal cells shape immunity by inducing MHC-I-dependent deletion of self-reactive CD8+T cells and MHC-II-dependent anergy of CD4+T cells. In this study, we show that MHC-II expression on lymph node stromal cells is additionally required for homeostatic maintenance of regulatory T cells (Tregs) and maintenance of immune quiescence. In the absence of MHC-II expression in lymph node transplants, i.e. on lymph node stromal cells, CD4+as well as CD8+T cells became activated, ultimately resulting in transplant rejection. MHC-II self-antigen presentation by lymph node stromal cells allowed the non-proliferative maintenance of antigen-specific Tregs and constrained antigen-specific immunity. Altogether, our results reveal a novel mechanism by which lymph node stromal cells regulate peripheral immunity.
authors.affiliation: Department of Molecular Cell Biology and Immunology, Vrije Universiteit Medical Center, Amsterdam, Netherlands.
authors.forename: Antonio P
authors.initials: AP
authors.lastname: Baptista
authors.affiliation: Department of Molecular Cell Biology and Immunology, Vrije Universiteit Medical Center, Amsterdam, Netherlands.
authors.forename: Ramon
authors.initials: R
authors.lastname: Roozendaal
authors.affiliation: Department of Molecular Cell Biology and Immunology, Vrije Universiteit Medical Center, Amsterdam, Netherlands.
authors.forename: Rogier M
authors.initials: RM
authors.lastname: Reijmers
authors.affiliation: Department of Molecular Cell Biology and Immunology, Vrije Universiteit Medical Center, Amsterdam, Netherlands.
authors.forename: Jasper J
authors.initials: JJ
authors.lastname: Koning
authors.affiliation: Department of Molecular Cell Biology and Immunology, Vrije Universiteit Medical Center, Amsterdam, Netherlands.
authors.forename: Wendy W
authors.initials: WW
authors.lastname: Unger
authors.affiliation: Department of Molecular Cell Biology and Immunology, Vrije Universiteit Medical Center, Amsterdam, Netherlands.
authors.forename: Mascha
authors.initials: M
authors.lastname: Greuter
authors.affiliation: Department of Molecular Cell Biology and Immunology, Vrije Universiteit Medical Center, Amsterdam, Netherlands.
authors.forename: Eelco D
authors.initials: ED
authors.lastname: Keuning
authors.affiliation: Department of Molecular Cell Biology and Immunology, Vrije Universiteit Medical Center, Amsterdam, Netherlands.
authors.forename: Rosalie
authors.initials: R
authors.lastname: Molenaar
authors.affiliation: Department of Molecular Cell Biology and Immunology, Vrije Universiteit Medical Center, Amsterdam, Netherlands.
authors.forename: Gera
authors.initials: G
authors.lastname: Goverse
authors.affiliation: Department of Molecular Cell Biology and Immunology, Vrije Universiteit Medical Center, Amsterdam, Netherlands.
authors.forename: Marlous M S
authors.initials: MM
authors.lastname: Sneeboer
authors.affiliation: Department of Molecular Cell Biology and Immunology, Vrije Universiteit Medical Center, Amsterdam, Netherlands.
authors.forename: Joke M M
authors.initials: JM
authors.lastname: den Haan
authors.affiliation: Department of Pediatric Immunology, Laboratory of Translational Immunology, University Medical Center Utrecht, Utrecht, Netherlands.
authors.forename: Marianne
authors.initials: M
authors.lastname: Boes
authors.affiliation: Department of Molecular Cell Biology and Immunology, Vrije Universiteit Medical Center, Amsterdam, Netherlands.
authors.forename: Reina E
authors.initials: RE
authors.lastname: Mebius
keywords: MHC-II; immunology; lymph node stromal cells; mouse; regulatory T cells