title: Pluripotent stem cell-derived neural stem cells: From basic research to applications.
abstract: Basic research on pluripotent stem cells is designed to enhance understanding of embryogenesis, whereas applied research is designed to develop novel therapies and prevent diseases. Attainment of these goals has been enhanced by the establishment of embryonic stem cell lines, the technological development of genomic reprogramming to generate induced-pluripotent stem cells, and improvements in vitro techniques to manipulate stem cells. This review summarizes the techniques required to generate neural cells from pluripotent stem cells. In particular, this review describes current research applications of a simple neural differentiation method, the neural stem sphere method, which we developed.
authors.affiliation: Masahiro Otsu, Department of Chemistry, Kyorin University School of Medicine, Shinkawa, Mitaka , Tokyo 181-8611, Japan.
authors.forename: Masahiro
authors.initials: M
authors.lastname: Otsu
authors.affiliation: Masahiro Otsu, Department of Chemistry, Kyorin University School of Medicine, Shinkawa, Mitaka , Tokyo 181-8611, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Nakayama
authors.affiliation: Masahiro Otsu, Department of Chemistry, Kyorin University School of Medicine, Shinkawa, Mitaka , Tokyo 181-8611, Japan.
authors.forename: Nobuo
authors.initials: N
authors.lastname: Inoue
keywords: Cell-based therapies; Embryonic stem cells; Neural Stem Sphere method; Neural stem cells; Pluripotent stem cells