title: C-deletion mutation of the p53 gene at exon 4 of codon 63 in the saliva of oral squamous cell carcinoma in central India: a preliminary study.
abstract: OBJECTIVE
  The purpose of this study was to detect the C-deletion mutation of the p53 gene at exon 4 of codon 63 in the saliva of oral squamous cell carcinoma in central India.
  
  
  METHODS
  The study was carried out in 30 oral squamous cell carcinoma cases and five healthy controls with no habit of betel nut and tobacco chewing. The C-deletion mutation of the p53 gene at exon 4 of codon 63 was detected in the saliva samples by using polymerase chain reaction.
  
  
  RESULTS
  In this study, C-deletion at exon 4 of codon 63 was detected in 28 of 30 oral squamous cell carcinoma cases (93.33%), but was negative in all five healthy controls and two oral squamous cell carcinoma cases.
  
  
  CONCLUSIONS
  The results indicated that C-deletion mutation at exon 4 of codon 63 of the p53 gene in the saliva might be a plausible molecular marker for oral squamous cell carcinoma patients with a habit of betel nut and tobacco lime quid chewing. The results further emphasize the presence of p53 gene mutation in patients with oral squamous cell carcinoma, which can be detected in the saliva through polymerase chain reaction.
authors.affiliation: Department of Oral and Maxillofacial Pathology and Microbiology, Sharad Pawar Dental College and Hospital, Datta Meghe Institute of Medical Sciences, Sawangi (Meghe) Wardha, Maharashtra, India.
authors.forename: Arti
authors.initials: A
authors.lastname: Mewara
authors.forename: Amol Ramchandra
authors.initials: AR
authors.lastname: Gadbail
authors.forename: Swati
authors.initials: S
authors.lastname: Patil
authors.forename: Minal
authors.initials: M
authors.lastname: Chaudhary
authors.forename: Shivkumar D
authors.initials: SD
authors.lastname: Chavhan
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001114:Areca; D014408:Biomarkers, Tumor / Q000032:analysis; D002294:Carcinoma, Squamous Cell / Q000235:genetics*; D003062:Codon / Q000235:genetics*; D003596:Cytosine; D005091:Exons / Q000235:genetics*; D005260:Female; D016158:Genes, p53 / Q000235:genetics*; D006801:Humans; D007194:India; D008297:Male; D008875:Middle Aged; D009061:Mouth Mucosa / Q000473:pathology; D009062:Mouth Neoplasms / Q000235:genetics*; D009754:Nuts; D016133:Polymerase Chain Reaction / Q000379:methods; D012463:Saliva / Q000737:chemistry*; D017384:Sequence Deletion / Q000235:genetics*; D014030:Tobacco, Smokeless; D016159:Tumor Suppressor Protein p53 / Q000235:genetics*; D055815:Young Adult
chemical_list: D014408:Biomarkers, Tumor; D003062:Codon; C495901:TP53 protein, human; D016159:Tumor Suppressor Protein p53; D003596:Cytosine
keywords: Indian subcontinent population; mutation-specific polymerase chain reaction; oral squamous cell carcinoma; p53 gene mutation; saliva