title: Nanoparticle assisted photothermal deformation of individual neuronal organelles and cells.
abstract: Stimulation of the localized surface plasmon of metallic nanoparticles has been shown to be an effective mechanism to induce photothermal damage in biological tissues. However, few studies have focused on single cell or subcellular ablation. Our results show that, upon incubation, gold nanostars are internalized by neurons of acute mouse cerebellar brain slices, clustering inside or close to the nucleus. By stimulating the nanostars' surface plasmon using a femtosecond laser, we show deformation of single nuclei and single cells. Given its precision and extremely localized effect, this is a promising technique for photothermal therapy in areas sensitive to collateral thermal damage such as the nervous system.
authors.affiliation: Centro de Investigaciones en Optica A. C., Leon, Gto, 37150 Mexico ; Universidad de Guadalajara, División de Ciencias, Centro Universitario de Tonalá, Tonalá Jalisco 48525 Mexico ; Equal contribution.
authors.forename: V H
authors.initials: VH
authors.lastname: Romero
authors.affiliation: Department of Physics and Astronomy, University of Texas at San Antonio, San Antonio, TX 78249 USA ; Equal contribution.
authors.forename: Z
authors.initials: Z
authors.lastname: Kereselidze
authors.affiliation: Department of Biology and Neurosciences Institute, University of Texas at San Antonio, San Antonio, TX 78249 USA ; Faculty of Medicine, Universidad Nacional Autonoma de Mexico, Mexico DF 07510 Mexico.
authors.forename: W
authors.initials: W
authors.lastname: Egido
authors.affiliation: Department of Biology and Neurosciences Institute, University of Texas at San Antonio, San Antonio, TX 78249 USA.
authors.forename: E A
authors.initials: EA
authors.lastname: Michaelides
authors.affiliation: Department of Biology and Neurosciences Institute, University of Texas at San Antonio, San Antonio, TX 78249 USA.
authors.forename: F
authors.initials: F
authors.lastname: Santamaria
authors.affiliation: Department of Physics and Astronomy, University of Texas at San Antonio, San Antonio, TX 78249 USA.
authors.forename: X G
authors.initials: XG
authors.lastname: Peralta
keywords: (160.4236) Nanomaterials; (170.1020) Ablation of tissue; (170.1530) Cell analysis; (240.6680) Surface plasmons