title: Expanding the chemical diversity of lasso peptide MccJ25 with genetically encoded noncanonical amino acids.
abstract: Using the amber suppression approach, four noncanonical amino acids (ncAAs) were used to replace existing amino acids at four positions in lasso peptide microcin J25 (MccJ25). The lasso peptide biosynthesis enzymes tolerated all four ncAAs and produced antibiotics with efficacy equivalent to wild-type in some cases. Given the rapid expansion of the genetically encoded ncAA pool, this study is the first to demonstrate an expedient method to significantly increase the chemical diversity of lasso peptides.
authors.affiliation: Department of Chemical and Biological Engineering, Princeton University, Princeton, NJ 08544, USA. ajlink@princeton.edu.
authors.forename: Frank J
authors.initials: FJ
authors.lastname: Piscotta
authors.forename: Jeffery M
authors.initials: JM
authors.lastname: Tharp
authors.forename: Wenshe R
authors.initials: WR
authors.lastname: Liu
authors.forename: A James
authors.initials: AJ
authors.lastname: Link
mesh_terms: D000081:Acetamides; D000596:Amino Acids / Q000737:chemistry* / Q000235:genetics; D000890:Anti-Infective Agents / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D001430:Bacteriocins / Q000737:chemistry* / Q000235:genetics; D004926:Escherichia coli / Q000737:chemistry* / Q000235:genetics; D005818:Genetic Engineering; D006801:Humans; D008958:Models, Molecular; D010455:Peptides; D012475:Salmonella / Q000187:drug effects; D012480:Salmonella Infections / Q000188:drug therapy
chemical_list: D000081:Acetamides; D000596:Amino Acids; D000890:Anti-Infective Agents; D001430:Bacteriocins; D010455:Peptides; C020411:microcin; C000188:alachlor