title: Evaluation of Phytocannabinoids from High PotencyCannabis sativausingIn VitroBioassays to Determine Structure-Activity Relationships for Cannabinoid Receptor 1 and Cannabinoid Receptor 2.
abstract: Cannabishas been around for thousands of years and has been used recreationally, medicinally, and for fiber. Over 500 compounds have been isolated fromCannabis sativawith approximately 105 being cannabinoids. Of those 105 compounds, Δ9-tetrahydrocannabinol has been determined as the primary constituent, which is also responsible for the psychoactivity associated withCannabis. Cannabinoid receptors belong to the large superfamily of G protein-coupled receptors. Targeting the cannabinoid receptors has the potential to treat a variety of conditions such as pain, neurodegeneration, appetite, immune function, anxiety, cancer, and others. Developingin vitrobioassays to determine binding and functional activity of compounds has the ability to lead researchers to develop a safe and effective drug that may target the cannabinoid receptors. Using radioligand binding and functional bioassays, a structure-activity relationship for major and minor cannabinoids was developed.
authors.affiliation: Department of Medicinal Chemistry, School of Pharmacy, University of Mississippi, University, MS 38677, USA.
authors.forename: Afeef S
authors.initials: AS
authors.lastname: Husni
authors.affiliation: Department of Medicinal Chemistry, School of Pharmacy, University of Mississippi, University, MS 38677, USA ; National Center for Natural Products Research, School of Pharmacy, University of Mississippi, University Mississippi 38677, USA.
authors.forename: Christopher R
authors.initials: CR
authors.lastname: McCurdy
authors.affiliation: National Center for Natural Products Research, School of Pharmacy, University of Mississippi, University Mississippi 38677, USA.
authors.forename: Mohamed M
authors.initials: MM
authors.lastname: Radwan
authors.affiliation: National Center for Natural Products Research, School of Pharmacy, University of Mississippi, University Mississippi 38677, USA.
authors.forename: Safwat A
authors.initials: SA
authors.lastname: Ahmed
authors.affiliation: National Center for Natural Products Research, School of Pharmacy, University of Mississippi, University Mississippi 38677, USA.
authors.forename: Desmond
authors.initials: D
authors.lastname: Slade
authors.affiliation: National Center for Natural Products Research, School of Pharmacy, University of Mississippi, University Mississippi 38677, USA ; Department of Pharmacognosy, School of Pharmacy, University of Mississippi, University, MS 38677, USA.
authors.forename: Samir A
authors.initials: SA
authors.lastname: Ross
authors.affiliation: National Center for Natural Products Research, School of Pharmacy, University of Mississippi, University Mississippi 38677, USA ; Department of Pharmaceutics, School of Pharmacy, University of Mississippi, University, MS 38677, USA.
authors.forename: Mahmoud A
authors.initials: MA
authors.lastname: ElSohly
authors.affiliation: Department of Medicinal Chemistry, School of Pharmacy, University of Mississippi, University, MS 38677, USA ; National Center for Natural Products Research, School of Pharmacy, University of Mississippi, University Mississippi 38677, USA.
authors.forename: Stephen J
authors.initials: SJ
authors.lastname: Cutler
keywords: Cannabis; cannabinoid; cannabinoid receptor 1; cannabinoid receptor 2; structure-activity relationship; tetrahydrocannabinol