title: Draft Genome Sequence of Escherichia coli Strain VKPM B-10182, Producing the Enzyme for Synthesis of Cephalosporin Acids.
abstract: Escherichia coli strain VKPM B-10182, obtained by chemical mutagenesis from E. coli strain ATCC 9637, produces cephalosporin acid synthetase employed in the synthesis of β-lactam antibiotics, such as cefazolin. The draft genome sequence of strain VKPM B-10182 revealed 32 indels and 1,780 point mutations that might account for the improvement in antibiotic synthesis that we observed.
authors.affiliation: Centre "Bioengineering," Russian Academy of Sciences, Moscow, Russian Federation.
authors.forename: Andrey V
authors.initials: AV
authors.lastname: Mardanov
authors.affiliation: Centre "Bioengineering," Russian Academy of Sciences, Moscow, Russian Federation.
authors.forename: Mikhail A
authors.initials: MA
authors.lastname: Eldarov
authors.affiliation: State Research Institute of Genetics and Selection of Industrial Microorganisms, Moscow, Russian Federation.
authors.forename: Anna V
authors.initials: AV
authors.lastname: Sklyarenko
authors.affiliation: Centre "Bioengineering," Russian Academy of Sciences, Moscow, Russian Federation.
authors.forename: Maria V
authors.initials: MV
authors.lastname: Dumina
authors.affiliation: Centre "Bioengineering," Russian Academy of Sciences, Moscow, Russian Federation.
authors.forename: Alexey V
authors.initials: AV
authors.lastname: Beletsky
authors.affiliation: State Research Institute of Genetics and Selection of Industrial Microorganisms, Moscow, Russian Federation.
authors.forename: Sergey V
authors.initials: SV
authors.lastname: Yarotsky
authors.affiliation: Centre "Bioengineering," Russian Academy of Sciences, Moscow, Russian Federation nravin@biengi.ac.ru.
authors.forename: Nikolai V
authors.initials: NV
authors.lastname: Ravin