title: β-Carboline alkaloids from Galianthe ramosa inhibit malate synthase from Paracoccidioides spp.
abstract: As part of our continuing chemical and biological analyses of Rubiaceae species from Cerrado, we isolated novel alkaloids 1 and 2, along with known compounds epicatechin, ursolic acid, and oleanolic acid, from Galianthe ramosa. Alkaloid 2 inhibited malate synthase from the pathogenic fungus Paracoccidioides spp. This enzyme is considered an important molecular target because it is not found in humans. Molecular docking simulations were used to describe the interactions between the alkaloids and malate synthase.
authors.affiliation: Instituto de Química - Universidade Federal de Goiás, Goiânia, GO, Brazil.
authors.forename: Carla S
authors.initials: CS
authors.lastname: de Freitas
authors.affiliation: Instituto de Química - Universidade Federal de Goiás, Goiânia, GO, Brazil.
authors.forename: Lucilia
authors.initials: L
authors.lastname: Kato
authors.affiliation: Instituto de Química - Universidade Federal de Goiás, Goiânia, GO, Brazil.
authors.forename: Cecília M A
authors.initials: CM
authors.lastname: de Oliveira
authors.affiliation: Instituto de Química - Universidade Federal de Goiás, Goiânia, GO, Brazil.
authors.forename: Luiz H K
authors.initials: LH
authors.lastname: Queiroz
authors.affiliation: Instituto de Química - Universidade Federal de Goiás, Goiânia, GO, Brazil.
authors.forename: Mábio J
authors.initials: MJ
authors.lastname: Santana
authors.affiliation: Depto. Química, Universidade Estadual de Maringá, Maringá, PR, Brazil.
authors.forename: Ivânia T
authors.initials: IT
authors.lastname: Schuquel
authors.affiliation: Herbier de Guyane, IRD, UMR AMAP, Cayenne, French Guiana, France.
authors.forename: Piero G
authors.initials: PG
authors.lastname: Delprete
authors.affiliation: Núcleo Colaborativo de BioSistemas, Campus Jataí, Universidade Federal de Goiás, Goiânia, GO, Brazil.
authors.forename: Roosevelt A
authors.initials: RA
authors.lastname: da Silva
authors.affiliation: Núcleo Colaborativo de BioSistemas, Campus Jataí, Universidade Federal de Goiás, Goiânia, GO, Brazil.
authors.forename: Guilherme O
authors.initials: GO
authors.lastname: Quintino
authors.affiliation: Instituto de Ciências Biológicas - Universidade Federal de Goiás, Goiânia, GO, Brazil.
authors.forename: Benedito R
authors.initials: BR
authors.lastname: da Silva Neto
authors.affiliation: Instituto de Ciências Biológicas - Universidade Federal de Goiás, Goiânia, GO, Brazil.
authors.forename: Célia M A
authors.initials: CM
authors.lastname: Soares
authors.affiliation: Instituto de Ciências Biológicas - Universidade Federal de Goiás, Goiânia, GO, Brazil.
authors.forename: Maristela
authors.initials: M
authors.lastname: Pereira
mesh_terms: D000470:Alkaloids / Q000737:chemistry / Q000494:pharmacology; D000935:Antifungal Agents / Q000737:chemistry / Q000494:pharmacology*; D002243:Carbolines / Q000737:chemistry / Q000494:pharmacology*; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000494:pharmacology*; D005656:Fungal Proteins / Q000378:metabolism; D020128:Inhibitory Concentration 50; D008292:Malate Synthase / Q000037:antagonists & inhibitors* / Q000737:chemistry / Q000378:metabolism; D008826:Microbial Sensitivity Tests; D062105:Molecular Docking Simulation; D015394:Molecular Structure; D010228:Paracoccidioides / Q000187:drug effects / Q000201:enzymology* / Q000472:pathogenicity; D035261:Plant Components, Aerial / Q000737:chemistry; D019666:Rubiaceae / Q000737:chemistry
chemical_list: D000470:Alkaloids; D000935:Antifungal Agents; D002243:Carbolines; D004791:Enzyme Inhibitors; D005656:Fungal Proteins; D008292:Malate Synthase