title: Cryopreservation of Greenshell™ mussel (Perna canaliculus) sperm.
abstract: Cryopreservation is a valuable technique for aquaculture as it enables a library or bank of genetically valuable animals to be maintained in a cost-effective manner. Here, we describe a method to cryopreserve the sperm of the Greenshell™ mussel (Perna canaliculus) and how to use the sperm post-thawing to maximize larval production from thawed sperm in selective breeding.
authors.affiliation: Cawthron Institute, 98 Halifax Street East, Private Bag 2, Nelson, 7042, New Zealand, serean.adams@cawthron.org.nz.
authors.forename: Serean L
authors.initials: SL
authors.lastname: Adams
authors.forename: John F
authors.initials: JF
authors.lastname: Smith
authors.forename: Jolene
authors.initials: J
authors.lastname: Taylor
authors.forename: Lindsay T
authors.initials: LT
authors.lastname: McGowan
authors.forename: H Robin
authors.initials: HR
authors.lastname: Tervit
mesh_terms: D000818:Animals; D001947:Breeding; D015925:Cryopreservation / Q000295:instrumentation / Q000379:methods / Q000662:veterinary*; D004867:Equipment Design; D005260:Female; D005306:Fertilization; D008297:Male; D049883:Perna / Q000502:physiology*; D012662:Semen Preservation / Q000295:instrumentation / Q000379:methods / Q000662:veterinary*; D013094:Spermatozoa / Q000166:cytology*