title: Genetic characteristics of the complete feline kobuvirus genome.
abstract: We sequenced the complete genome of a feline kobuvirus and determined relationships with other kobuviruses. This kobuvirus has an 8,269-nucleotide-long RNA genome, excluding the poly(A) tail. The genome contains a 7,311-bp open reading frame (ORF) encoding a putative polyprotein precursor of 2,437 amino acids, a 717-bp 5'-untranslated region (UTR), and a 241-bp 3'-UTR. The L protein sequence was found to be the most variable region in the feline kobuvirus genome. Interestingly, the 5'-UTR B and C stem-loops were conserved as observed with other kobuviruses; however, a secondary structure corresponding to stem-loop A was not found in the full length 5'-UTR sequence. Phylogenetic tree analysis showed that kobuviruses can be divided into 3 main groups. The feline kobuvirus belongs to the Aichivirus A species containing Aichivirus, mouse kobuvirus, and canine kobuvirus.
authors.affiliation: Viral Disease Diagnostic Laboratory, Animal Disease Diagnostic Division, Animal and Plant Quarantine Agency, 480 Anyang-6-Dong, Anyang, 430-824, Republic of Korea.
authors.forename: Jeong-Won
authors.initials: JW
authors.lastname: Choi
authors.forename: Myoung-Heon
authors.initials: MH
authors.lastname: Lee
authors.forename: Kyoung-Ki
authors.initials: KK
authors.lastname: Lee
authors.forename: Jae-Ku
authors.initials: JK
authors.lastname: Oem
mesh_terms: D020413:3' Untranslated Regions; D020121:5' Untranslated Regions; D000818:Animals; D002415:Cats / Q000821:virology; D016000:Cluster Analysis; D016679:Genome, Viral; D052699:Kobuvirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D016366:Open Reading Frames; D010802:Phylogeny; D020815:Polyproteins / Q000235:genetics; D012367:RNA, Viral / Q000235:genetics*; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D014764:Viral Proteins / Q000235:genetics
chemical_list: D020413:3' Untranslated Regions; D020121:5' Untranslated Regions; D020815:Polyproteins; D012367:RNA, Viral; D014764:Viral Proteins