title: Transient activation of an adaptor protein, disabled-2, in rat spinal cord injury.
abstract: We previously reported that disabled-2 (Dab-2), a cytosolic adaptor protein, was expressed in inflammatory and glial cells in the central nervous system (CNS) in experimental autoimmune encephalomyelitis and cerebral cryoinjury. Here, to determine the pattern of Dab-2 expression in a clip compression-induced rat spinal cord injury (SCI) model, the protein level and localization of Dab-2 in the spinal cord were investigated in rats with SCI using Western blotting and immunohistochemistry. Western blotting revealed that the expression of both the 75- and 100-kDa isoforms of Dab-2 peaked significantly in the spinal cord after clip compression injury 7 days post-injury compared to sham controls, and declined slightly thereafter. Immunohistochemistry revealed weak Dab-2 immunostaining in some neurons, glial cells, and ependymal cells in the spinal cords of the control animals, compared to staining in the macrophages and reactive astrocytes in lesions of the SCI animals. Overall, these findings suggest that both isoforms of Dab-2 are transiently upregulated in response to SCI and that the increased expression of Dab-2 is associated with the early activation of macrophages and astrogliosis in the course of CNS inflammation.
authors.forename: Meejung
authors.initials: M
authors.lastname: Ahn
authors.forename: Changjong
authors.initials: C
authors.lastname: Moon
authors.forename: Changnam
authors.initials: C
authors.lastname: Park
authors.forename: Jeongtae
authors.initials: J
authors.lastname: Kim
authors.forename: Ki-Bum
authors.initials: KB
authors.lastname: Sim
authors.forename: Taekyun
authors.initials: T
authors.lastname: Shin
mesh_terms: D033942:Adaptor Proteins, Vesicular Transport / Q000096:biosynthesis*; D000818:Animals; D001253:Astrocytes / Q000378:metabolism / Q000473:pathology; D050815:Fractures, Compression / Q000378:metabolism* / Q000473:pathology; D005786:Gene Expression Regulation; D007249:Inflammation / Q000378:metabolism / Q000473:pathology; D008262:Macrophage Activation; D008264:Macrophages / Q000378:metabolism / Q000473:pathology; D008297:Male; D020033:Protein Isoforms / Q000096:biosynthesis; D051381:Rats; D017207:Rats, Sprague-Dawley; D013119:Spinal Cord Injuries / Q000378:metabolism* / Q000473:pathology
chemical_list: D033942:Adaptor Proteins, Vesicular Transport; C501404:Dab2 protein, rat; D020033:Protein Isoforms