title: Differential protein expression of Caco-2 cells treated with selenium nanoparticles compared with sodium selenite and selenomethionine.
abstract: The study was designed to determine the differential protein expression of Caco-2 cells treated with different forms of selenium including sodium selenite, selenomethionine (Se-Met), and selenium nanoparticles (nano-Se). Two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) and mass spectrometry (MS) were used to identify the differentially expressed proteins. The results indicated that seven protein spots, ubiquitin-conjugating enzyme E2 (E2), glutathione synthetases (GS), triosephosphate isomerase (TSP), T-complex protein 1 subunit zeta (TCPZ), lamin-B1, heterogeneous nuclear ribonucleoprotein F (hnRNP F), and superoxide dismutase [Cu-Zn] (Cu, Zn-SOD) were significantly different among all the groups. According to the order of control, sodium selenite, Se-Met, and Nano-Se, the expression levels of two proteins (E2 and GS) increased and the other differential proteins were reverse. Except for E2, there were no significant differences in other protein expressions between the groups treated with nano-Se and Se-Met.
authors.affiliation: Key Laboratory for Food Microbial Technology of Zhejiang Province, Food Quality and Safety Department of Zhejiang Gongshang University, 18 Xuezheng Road, Xiasha University Town, Hangzhou 310018, China.
authors.forename: Linglin
authors.initials: L
authors.lastname: Fu
authors.affiliation: Key Laboratory for Food Microbial Technology of Zhejiang Province, Food Quality and Safety Department of Zhejiang Gongshang University, 18 Xuezheng Road, Xiasha University Town, Hangzhou 310018, China.
authors.forename: Xuxia
authors.initials: X
authors.lastname: Yan
authors.affiliation: College of Life Sciences, Zhejiang University of Traditional Chinese Medicine, 548, Binwen Road, Binjiang District, Hangzhou 310053, China.
authors.forename: Xinming
authors.initials: X
authors.lastname: Ruan
authors.affiliation: Department of Biological Sciences, Florida Institute of Technology, 150 W. University Blvd., Melbourne, FL 32901, USA.
authors.forename: Junda
authors.initials: J
authors.lastname: Lin
authors.affiliation: Key Laboratory for Food Microbial Technology of Zhejiang Province, Food Quality and Safety Department of Zhejiang Gongshang University, 18 Xuezheng Road, Xiasha University Town, Hangzhou 310018, China.
authors.forename: Yanbo
authors.initials: Y
authors.lastname: Wang
keywords: Proteomics; Selenium nanoparticle; Selenomethionine; Sodium selenite