title: Protective effect of resveratrol against cisplatin-induced ototoxicity in HEI-OC1 auditory cells.
abstract: OBJECTIVE
  Cisplatin is an effective chemotherapeutic drug, but it generates reactive oxygen species (ROS) that induce severe adverse effects such as ototoxicity. Resveratrol reportedly prevents oxidative stress-induced cell death. Thus, we hypothesized that the anti-oxidative effect of resveratrol could protect against cisplatin-induced ototoxicity. The present study examined the protective effect of resveratrol against cisplatin-induced ototoxicity in HEI-OC1 auditory cells.
  
  
  METHODS
  HEI-OC1 cells were pretreated with resveratrol at 1μM for 24h and then exposed to 15μM cisplatin for 48h. Resulting cytotoxicity was measured by the MTT method, and intracellular ROS was measured using flow cytometry. Protective effect of resveratrol was compared with other anti-oxidants.
  
  
  RESULTS
  Pretreatment with resveratrol 1μM protected HEI-OC1 auditory cells against cisplatin-induced cytotoxicity and significantly reduced a cisplatin-induced increase in ROS. Resveratrol provided significant protection against 15μM cisplatin applied for 48h (50.8% cell viability in the cisplatin group vs. 57.6% in the cisplatin-plus-resveratrol group), and there was a 9% decrease in cisplatin-induced ROS associated with resveratrol.
  
  
  CONCLUSIONS
  This is the study investigating the protective effects of resveratrol against cisplatin-induced ototoxicity in an auditory cell line. Resveratrol significantly reduced a cisplatin-induced increase in ROS and thereby inhibited cisplatin-induced cytotoxicity.
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, Korea University College of Medicine, Inchon-ro 73, Seongbuk-Gu, 136-705 Seoul, Republic of Korea. Electronic address: lshe5@naver.com.
authors.forename: Se Hee
authors.initials: SH
authors.lastname: Lee
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, Korea University College of Medicine, Inchon-ro 73, Seongbuk-Gu, 136-705 Seoul, Republic of Korea. Electronic address: hyungsubk@gmail.com.
authors.forename: Hyung Sub
authors.initials: HS
authors.lastname: Kim
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, Korea University College of Medicine, Inchon-ro 73, Seongbuk-Gu, 136-705 Seoul, Republic of Korea. Electronic address: stella0423@hotmail.com.
authors.forename: Yun Suk
authors.initials: YS
authors.lastname: An
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, Korea University College of Medicine, Inchon-ro 73, Seongbuk-Gu, 136-705 Seoul, Republic of Korea. Electronic address: brune77@naver.com.
authors.forename: Jiwon
authors.initials: J
authors.lastname: Chang
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, Korea University College of Medicine, Inchon-ro 73, Seongbuk-Gu, 136-705 Seoul, Republic of Korea. Electronic address: mednlaw1@gmail.com.
authors.forename: June
authors.initials: J
authors.lastname: Choi
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, Korea University College of Medicine, Inchon-ro 73, Seongbuk-Gu, 136-705 Seoul, Republic of Korea. Electronic address: logopas@korea.ac.kr.
authors.forename: Gi Jung
authors.initials: GJ
authors.lastname: Im
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000009:adverse effects*; D000975:Antioxidants / Q000494:pharmacology*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D002945:Cisplatin / Q000009:adverse effects*; D006198:Hair Cells, Auditory / Q000187:drug effects*; D008822:Mice, Transgenic; D017382:Reactive Oxygen Species / Q000032:analysis; D000077185:Resveratrol; D013267:Stilbenes / Q000494:pharmacology*
chemical_list: D000970:Antineoplastic Agents; D000975:Antioxidants; D017382:Reactive Oxygen Species; D013267:Stilbenes; D002945:Cisplatin; D000077185:Resveratrol
keywords: Cisplatin; Ototoxicity; Reactive oxygen species; Resveratrol