title: Nicotinic acid increases adiponectin secretion from differentiated bovine preadipocytes through G-protein coupled receptor signaling.
abstract: The transition period in dairy cows (3 weeks prepartum until 3 weeks postpartum) is associated with substantial mobilization of energy stores, which is often associated with metabolic diseases. Nicotinic acid (NA) is an antilipolytic and lipid-lowering compound used to treat dyslipidaemia in humans, and it also reduces non-esterified fatty acids in cattle. In mice the G-protein coupled receptor 109A (GPR109A) ligand NA positively affects the secretion of adiponectin, an important modulator of glucose and fat metabolism. In cattle, the corresponding data linking NA to adiponectin are missing. Our objective was to examine the effects of NA on adiponectin and AMPK protein abundance and the expression of mRNAs of related genes such as chemerin, an adipokine that enhances adiponectin secretion in vitro. Differentiated bovine adipocytes were incubated with pertussis toxin (PTX) to verify the involvement of GPR signaling, and treated with 10 or 15 µM NA for 12 or 24 h. NA increased adiponectin concentrations (p ≤ 0.001) and the mRNA abundances of GPR109A (p ≤ 0.05) and chemerin (p ≤ 0.01). Pre-incubation with PTX reduced the adiponectin response to NA (p ≤ 0.001). The NA-stimulated secretion of adiponectin and the mRNA expression of chemerin in the bovine adipocytes were suggestive of GPR signaling-dependent improved insulin sensitivity and/or adipocyte metabolism in dairy cows.
authors.affiliation: Institute of Animal Science, Physiology & Hygiene Unit, University of Bonn, 53115 Bonn, Germany. christina.kopp@uni-bonn.de.
authors.forename: Christina
authors.initials: C
authors.lastname: Kopp
authors.affiliation: Institute of Animal Science, Physiology & Hygiene Unit, University of Bonn, 53115 Bonn, Germany. afshin.hosseini@gmail.com.
authors.forename: Afshin
authors.initials: A
authors.lastname: Hosseini
authors.affiliation: Institute of Animal Science, Physiology & Hygiene Unit, University of Bonn, 53115 Bonn, Germany. spsinghmail1@gmail.com.
authors.forename: Shiva P
authors.initials: SP
authors.lastname: Singh
authors.affiliation: Institute of Animal Science, Physiology & Hygiene Unit, University of Bonn, 53115 Bonn, Germany. Petra.Regenhard@lohmann.de.
authors.forename: Petra
authors.initials: P
authors.lastname: Regenhard
authors.affiliation: Institute of Animal Science, Physiology & Hygiene Unit, University of Bonn, 53115 Bonn, Germany. khalilvandi@ut.ac.ir.
authors.forename: Hamed
authors.initials: H
authors.lastname: Khalilvandi-Behroozyar
authors.affiliation: Institute of Animal Science, Physiology & Hygiene Unit, University of Bonn, 53115 Bonn, Germany. sauerwein@uni-bonn.de.
authors.forename: Helga
authors.initials: H
authors.lastname: Sauerwein
authors.affiliation: Institute of Animal Science, Physiology & Hygiene Unit, University of Bonn, 53115 Bonn, Germany. mielenz@fbn-dummerstorf.de.
authors.forename: Manfred
authors.initials: M
authors.lastname: Mielenz
mesh_terms: D055372:AMP-Activated Protein Kinases / Q000378:metabolism; D017667:Adipocytes / Q000187:drug effects* / Q000378:metabolism; D052242:Adiponectin / Q000378:metabolism*; D000818:Animals; D002417:Cattle; D002454:Cell Differentiation / Q000187:drug effects*; D007333:Insulin Resistance / Q000502:physiology; D009525:Niacin / Q000494:pharmacology*; D012333:RNA, Messenger / Q000378:metabolism; D043562:Receptors, G-Protein-Coupled / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects
chemical_list: D052242:Adiponectin; D012333:RNA, Messenger; D043562:Receptors, G-Protein-Coupled; D009525:Niacin; D055372:AMP-Activated Protein Kinases