title: A case of IMP-4-, OXA-421-, OXA-96-, and CARB-2-producing Acinetobacter pittii sequence type 119 in Australia.
abstract: An IMP-4-producing Acinetobacter pittii strain coproducing oxacillinases was isolated from a leg wound of a 67-year-old female patient. Identification to the species level by rpoB and gyrB sequencing and multiplex-PCR-based analysis revealed that the isolate was A. pittii. Whole-genome sequencing of this A. pittii isolate determined the presence of blaOXA-96, blaCARB-2, and a novel blaOXA-421 gene. The position of this novel blaOXA-421 gene was similar to that of blaOXA-51 in A. baumannii, downstream of the phosphinothricin N-acetyltransferase gene and upstream of fxsA in the chromosome. This A. pittii isolate was found to belong to sequence type 119 (ST119). Here, we report the first isolation of IMP-4-producing A. pittii ST119 with a novel blaOXA-421 gene from a patient in Australia and characterize its draft genome.
authors.affiliation: The University of Queensland, UQ Centre for Clinical Research, Brisbane, Queensland, Australia.
authors.forename: Witchuda
authors.initials: W
authors.lastname: Kamolvit
authors.affiliation: Pathology Queensland, Herston, Queensland, Australia.
authors.forename: Petra
authors.initials: P
authors.lastname: Derrington
authors.affiliation: The University of Queensland, UQ Centre for Clinical Research, Brisbane, Queensland, Australia Pathology Queensland, Herston, Queensland, Australia.
authors.forename: David L
authors.initials: DL
authors.lastname: Paterson
authors.affiliation: The University of Queensland, UQ Centre for Clinical Research, Brisbane, Queensland, Australia h.sidjabat@uq.edu.au.
authors.forename: Hanna E
authors.initials: HE
authors.lastname: Sidjabat
mesh_terms: D000150:Acinetobacter / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification*; D000151:Acinetobacter Infections / Q000175:diagnosis* / Q000382:microbiology*; D000368:Aged; D001315:Australia; D027081:DNA Gyrase / Q000235:genetics; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D012321:DNA-Directed RNA Polymerases / Q000235:genetics; D005260:Female; D016680:Genome, Bacterial; D006801:Humans; D008969:Molecular Sequence Data; D058885:Multilocus Sequence Typing; D060885:Multiplex Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D014946:Wound Infection / Q000175:diagnosis* / Q000382:microbiology*; D001618:beta-Lactamases / Q000235:genetics / Q000378:metabolism*
chemical_list: D004269:DNA, Bacterial; D012321:DNA-Directed RNA Polymerases; C105078:RNA polymerase beta subunit; D001618:beta-Lactamases; D027081:DNA Gyrase