title: Oxidation of glycosaminoglycans by free radicals and reactive oxidative species: A review of investigative methods.
abstract: Glycosaminoglycans, in particular hyaluronan (HA), and proteoglycans are components of the extracellular matrix (ECM). The ECM plays a key role in the regulation of cellular behaviour and alterations to it can modulate both the development of human diseases as well as controlling normal biochemical processes such as cell signalling and pro-inflammatory responses. For these reasons, in vitro fragmentation studies of glycosaminoglycans by free radicals and oxidative species are seen to be relevant to the understanding of in vivo studies of damage to the ECM. A wide range of investigative techniques have therefore been applied to gain insights into the relative fragmentation effects of several reactive oxidative species with the ultimate goal of determining mechanisms of fragmentation at the molecular level. These methods are reviewed here.
authors.affiliation: Health and Social Sciences, Leeds Beckett University , Leeds , UK.
authors.forename: B J
authors.initials: BJ
authors.lastname: Parsons
mesh_terms: D000818:Animals; D002623:Chemistry Techniques, Analytical; D002850:Chromatography, Gel; D004548:Elasticity; D004586:Electrophoresis; D005109:Extracellular Matrix / Q000737:chemistry / Q000378:metabolism; D005609:Free Radicals / Q000737:chemistry* / Q000378:metabolism; D006025:Glycosaminoglycans / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D007700:Kinetics; D008027:Light; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D012542:Scattering, Radiation; D015398:Signal Transduction; D014783:Viscosity
chemical_list: D005609:Free Radicals; D006025:Glycosaminoglycans
keywords: fragmentation; free radicals; glycosaminoglycans