title: Complete genome sequences and phylogenetic analysis of two West Nile virus strains isolated from equines in Argentina in 2006 could indicate an early introduction of the virus in the Southern Cone.
abstract: The complete nucleotide sequences of two West Nile virus (WNV) strains isolated in Argentina were determined. Phylogenetic trees were constructed from the aligned nucleic acid sequences of these two strains along with other previously published complete WNV genome sequences. Phylogenetic data showed that both strains belonged to clade 1a of lineage 1 and clustered in a subclade with American strains isolated during 1999-2002. These results suggest two independent routes of introduction of WNV in Argentina and that the virus could have been circulating in Argentina for some time before being isolated.
authors.affiliation: 1 Instituto Nacional de Enfermedades Virales Humanas "Dr. Julio I. Maiztegui" , Pergarmino, Argentina .
authors.forename: Cintia M
authors.initials: CM
authors.lastname: Fabbri
authors.forename: Jorge B
authors.initials: JB
authors.lastname: García
authors.forename: María Alejandra
authors.initials: MA
authors.lastname: Morales
authors.forename: Delia A
authors.initials: DA
authors.lastname: Enría
authors.forename: Silvana
authors.initials: S
authors.lastname: Levis
authors.forename: Robert S
authors.initials: RS
authors.lastname: Lanciotti
mesh_terms: D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D000818:Animals; D001118:Argentina / Q000453:epidemiology; D001483:Base Sequence; D001921:Brain / Q000821:virology; D002522:Chlorocebus aethiops; D016679:Genome, Viral / Q000235:genetics*; D005838:Genotype; D006734:Horse Diseases / Q000453:epidemiology / Q000821:virology; D006736:Horses; D010802:Phylogeny; D012367:RNA, Viral / Q000737:chemistry / Q000235:genetics; D016415:Sequence Alignment; D017423:Sequence Analysis, RNA; D014709:Vero Cells; D014901:West Nile Fever / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology; D014902:West Nile virus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification
chemical_list: D012367:RNA, Viral
keywords: Argentina; Equine strain sequences; Flavivirus; West Nile virus