title: Antioxidant and antifatigue activities of Polygonatum Alte-lobatum Hayata rhizomes in rats.
abstract: Polygonatum alte-lobatum Hayata, a rhizomatous perennial herb, belongs to the Liliaceae family and is endemic to Taiwan. We investigated the antioxidant and anti-fatigue activities of P. alte-lobatum in exercised rats. Levels of polyphenols, flavonoids and polysaccharides and 2,2-diphenyl-1-picrylhydrazyl (DPPH) free-radical scavenging activity were measured in extracts of P. alte-lobatum (EPA). Sprague-Dawley rats were randomly divided into four groups for 8-week treatment with vehicle (control) and low-, medium-, and high-dose EPA (LEPA, MEPA, HEPA; 0, 75, 150, and 375 mg/kg/day, respectively). Exercise performance was evaluated by exhaustive treadmill exercise time and by changes in body composition and biochemical variables at the end of the experiment. EPA contained polyphenols, flavonoids and polysaccharides, with polysaccharide content at least 26 times greater than that of polyphenols and flavonoids. Trend analysis revealed that EPA dose-dependently scavenged DPPH free radicals. EPA treatment dose-dependently increased endurance running time to exhaustion and superoxide dismutase activity and total antioxidant ability of blood. EPA dose-dependently decreased serum urea nitrogen and malondialdehyde levels after exercise. Hepatic glycogen content, an important energy source for exercise, was significantly increased with EPA treatment. EPA could be a potential agent with an anti-fatigue pharmacological function.
authors.affiliation: Department of Ophthalmology and Medical Education Center, Kaohsiung Armed Force General Hospital, Kaohsiung 80284, Taiwan. h560411@ms5.hinet.net.
authors.forename: Chi-Ting
authors.initials: CT
authors.lastname: Horng
authors.affiliation: Department of Pharmacy & Graduate Institute of Pharmaceutical Technology, Tajen University, Pingtung 90741, Taiwan. born.song@gmail.com.
authors.forename: Jon-Kway
authors.initials: JK
authors.lastname: Huang
authors.affiliation: Department of Pharmacy & Graduate Institute of Pharmaceutical Technology, Tajen University, Pingtung 90741, Taiwan. ppig8@yahoo.com.tw.
authors.forename: Hui-Yun
authors.initials: HY
authors.lastname: Wang
authors.affiliation: Graduate Institute of Sports Science, National Taiwan Sport University, Taoyuan 33301, Taiwan. john5523@ntsu.edu.tw.
authors.forename: Chi-Chang
authors.initials: CC
authors.lastname: Huang
authors.affiliation: Department of Pharmacy & Graduate Institute of Pharmaceutical Technology, Tajen University, Pingtung 90741, Taiwan. fachen.tajen@yahoo.com.tw.
authors.forename: Fu-An
authors.initials: FA
authors.lastname: Chen
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001713:Biphenyl Compounds / Q000032:analysis / Q000494:pharmacology; D001806:Blood Urea Nitrogen; D004305:Dose-Response Relationship, Drug; D005221:Fatigue / Q000188:drug therapy*; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D010805:Physical Conditioning, Animal; D010851:Picrates / Q000032:analysis / Q000494:pharmacology; D010936:Plant Extracts / Q000494:pharmacology; D031384:Polygonatum / Q000737:chemistry*; D059808:Polyphenols / Q000032:analysis / Q000494:pharmacology; D011134:Polysaccharides / Q000032:analysis / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D027343:Rhizome / Q000737:chemistry*; D013482:Superoxide Dismutase / Q000097:blood; D013624:Taiwan
chemical_list: D000975:Antioxidants; D001713:Biphenyl Compounds; D010851:Picrates; D010936:Plant Extracts; D059808:Polyphenols; D011134:Polysaccharides; D008315:Malondialdehyde; C004931:1,1-diphenyl-2-picrylhydrazyl; D013482:Superoxide Dismutase