title: Bacillus subtilis degSU operon is regulated by the ClpXP-Spx regulated proteolysis system.
abstract: The DegS-DegU two-component regulatory system regulates many cellular events in Bacillus subtilis. Genes for DegSU constitutes an operon directed by the P1 promoter and downstream degU is autoregulated via the P3 promoter activated by phosphorylated DegU. In the Gram-positive bacteria, Spx plays a major role in the protection system against oxidative stresses as a transcriptional regulator. Spx is a substrate of the ATP-dependent ClpXP protease. It regulates diamide-stress regulon in addition to many genes with unknown functions. We have found that null mutations for clpX and clpP, which encode the subunits for the protease ClpXP, enhanced the DegU level through activation of the P1 promoter. We isolated four suppressors for the clpP-enhancing effect. Whole-genome sequencing of the suppressors revealed that two have a point mutation in spx and the rest have a deletion of spx. The clpP-enhancing effect on degS-lacZ expression was abolished in the spx disruptant. These results show that the degSU operon is a new target of Spx-mediated positive regulation. Furthermore, we found that the P1 promoter was induced by glucose and that this induction was greatly reduced in the spx mutant. These results suggested that Spx-mediated glucose induction at the P1 promoter.
authors.affiliation: Genome Research Center, NODAI Research Institute, Tokyo University of Agriculture, 1-1-1 Sakuragaoka, Tokyo 156-8502, Japan and Institute of Oceanic Research and Development, Tokai University, 3-20-1 Orido-Shimizu, Shizuoka 424-8610, Japan.
authors.forename: Yuh
authors.initials: Y
authors.lastname: Shiwa
authors.affiliation: Genome Research Center, NODAI Research Institute, Tokyo University of Agriculture, 1-1-1 Sakuragaoka, Tokyo 156-8502, Japan and Institute of Oceanic Research and Development, Tokai University, 3-20-1 Orido-Shimizu, Shizuoka 424-8610, Japan.
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Yoshikawa
authors.affiliation: Genome Research Center, NODAI Research Institute, Tokyo University of Agriculture, 1-1-1 Sakuragaoka, Tokyo 156-8502, Japan and Institute of Oceanic Research and Development, Tokai University, 3-20-1 Orido-Shimizu, Shizuoka 424-8610, Japan.
authors.forename: Teruo
authors.initials: T
authors.lastname: Tanaka
authors.affiliation: Genome Research Center, NODAI Research Institute, Tokyo University of Agriculture, 1-1-1 Sakuragaoka, Tokyo 156-8502, Japan and Institute of Oceanic Research and Development, Tokai University, 3-20-1 Orido-Shimizu, Shizuoka 424-8610, Japan oguram@scc.u-tokai.ac.jp.
authors.forename: Mitsuo
authors.initials: M
authors.lastname: Ogura
mesh_terms: D001412:Bacillus subtilis / Q000235:genetics*; D001426:Bacterial Proteins / Q000502:physiology*; D005947:Glucose / Q000008:administration & dosage; D009154:Mutation; D009876:Operon; D011401:Promoter Regions, Genetic; D059748:Proteolysis
chemical_list: D001426:Bacterial Proteins; D005947:Glucose
keywords: Spx; glucose induction; proteolysis; response regulator; whole-genome sequencing