title: Separation and determination of reduced vitamin C in polymerized hemoglobin-based oxygen carriers of the human placenta.
abstract: The molybdenum blue method was used to determine the content of reduced vitamin C (Vc) in a solution of polymerized hemoglobin-based oxygen carriers (HBOCs) of the human placenta. The conditions of absorption wavelength, HCl addition, and reaction time, were investigated. The results of validation experiments showed that under the optimized conditions, a standard curve was confirmed with good linearity of 0.9985, for the Vc amount ranging from 0-200 μg. The values for relative standard deviation (RSD) of the precision and repeatability were both below 5%. Vc recovery was in the range of 97-102%. The conclusion could be made that a reduction in Vc content could be tested effectively by the molybdenum blue method.
authors.affiliation: Institute of Blood Transfusion, Chinese Academy of Medical Science , Chengdu , P. R. China.
authors.forename: Gang
authors.initials: G
authors.lastname: Chen
authors.forename: Ling
authors.initials: L
authors.lastname: Mo
authors.forename: Shen
authors.initials: S
authors.lastname: Li
authors.forename: Wentao
authors.initials: W
authors.lastname: Zhou
authors.forename: Hong
authors.initials: H
authors.lastname: Wang
authors.forename: Jiaxin
authors.initials: J
authors.lastname: Liu
authors.forename: Chengmin
authors.initials: C
authors.lastname: Yang
mesh_terms: D001205:Ascorbic Acid / Q000032:analysis*; D001802:Blood Substitutes / Q000737:chemistry*; D005260:Female; D006454:Hemoglobins / Q000737:chemistry*; D006801:Humans; D010084:Oxidation-Reduction; D010100:Oxygen; D010920:Placenta / Q000737:chemistry*; D011247:Pregnancy; D055503:Protein Multimerization
chemical_list: D001802:Blood Substitutes; D006454:Hemoglobins; D001205:Ascorbic Acid; D010100:Oxygen
keywords: Vitamin C; hemoglobin-based oxygen carriers; molybdenum blue method; spectrometry