title: Microvascular plasticity after experimental stroke: a molecular and MRI study.
abstract: BACKGROUND
  Microvasculature plays a key role in stroke pathophysiology both during initial damage and extended neural repair. Moreover, angiogenesis processes seem to be a promising target for future neurorestorative therapies. However, dynamic changes of microvessels after stroke still remain unclear, and MRI follow-up could be interesting as an in vivo biomarker of these.
  
  
  METHODS
  The aim of this study is to characterize the microvascular plasticity 25 days after ischemic stroke using both in vivo microvascular 7T-MRI (vascular permeability, cerebral blood volume (CBV), vessel size index (VSI), vascular density) and quantification of angiogenic factor expressions by RT-qPCR in a transient middle cerebral artery occlusion rat model. CBV and VSI (perfused vessel caliber) imaging was performed using a steady-state approach with a multi gradient-echo spin-echo sequence before and 2 min after intravenous (IV) injection of ultrasmall superparamagnetic iron particles. Vascular density (per mm2) was derived from the ratio [ΔR₂/(ΔR₂*)²/³]. Blood brain barrier leakage was assessed using T₁W images before and after IV injection of Gd-DOTA. Additionally, microvessel immunohistology was done.
  
  
  RESULTS
  3 successive stages were observed: 1) 'Acute stage' from day 1 to day 3 post-stroke (D1-D3) characterized by high levels of angiopoietin-2 (Ang2), vascular endothelial growth factor receptor-2 (VEGFR-2) and endothelial NO synthase (eNOS) that may be associated with deleterious vascular permeability and vasodilation; 2) 'Transition stage' (D3-D7) that involves transforming the growth factors β1 (TGFβ1), Ang1, and tyrosine kinase with immunoglobulin-like and endothelial growth factor-like domains 1 (Tie1), stromal-derived factor-1 (SDF-1), chemokine receptor type 4 (CXCR-4); and 3) 'Subacute stage' (D7-D25) with high levels of Ang1, Ang2, VEGF, VEGFR-1 and TGFβ1 leading to favorable stabilization and maturation of microvessels. In vivo MRI appeared in line with the angiogenic factors changes with a delay of at least 1 day. All MRI parameters varied over time, revealing the different aspects of the post-stroke microvascular plasticity. At D25, despite a normal CBV, MRI revealed a limited microvessel density, which is insufficient to support a good neural repair.
  
  
  CONCLUSIONS
  Microvasculature MRI can provide imaging of different states of functional (perfused) microvessels after stroke. These results highlight that multiparametric MRI is useful to assess post-stroke angiogenesis, and could be used as a biomarker notably for neurorestorative therapy studies. Additionally, we identified that endogenous vessel maturation and stabilization occur during the 'subacute stage'. Thus, pro-angiogenic treatments, such as cell-based therapy, would be relevant during this subacute phase of stroke.
authors.forename: Anaick
authors.initials: A
authors.lastname: Moisan
authors.forename: Isabelle M
authors.initials: IM
authors.lastname: Favre
authors.forename: Claire
authors.initials: C
authors.lastname: Rome
authors.forename: Emmanuelle
authors.initials: E
authors.lastname: Grillon
authors.forename: Bernadette
authors.initials: B
authors.lastname: Naegele
authors.forename: Marianne
authors.initials: M
authors.lastname: Barbieux
authors.forename: Florence
authors.initials: F
authors.lastname: De Fraipont
authors.forename: Marie-Jeanne
authors.initials: MJ
authors.lastname: Richard
authors.forename: Emmanuel L
authors.initials: EL
authors.lastname: Barbier
authors.forename: Chantal
authors.initials: C
authors.lastname: Rémy
authors.forename: Olivier
authors.initials: O
authors.lastname: Detante
mesh_terms: D000818:Animals; D001812:Blood-Brain Barrier / Q000473:pathology; D002199:Capillary Permeability; D004195:Disease Models, Animal; D020244:Infarction, Middle Cerebral Artery / Q000473:pathology; D008279:Magnetic Resonance Imaging / Q000379:methods; D008297:Male; D055806:Microvessels / Q000473:pathology*; D017207:Rats, Sprague-Dawley; D020521:Stroke / Q000150:complications / Q000473:pathology*; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism
chemical_list: D042461:Vascular Endothelial Growth Factor A