title: Application of tissue mesodissection to molecular cancer diagnostics.
abstract: OBJECTIVE
  To demonstrate clinical application of a mesodissection platform that was developed to combine advantages of laser-based instrumentation with the speed/ease of manual dissection for automated dissection of tissue off standard glass slides.
  
  
  METHODS
  Genomic analysis for KRAS gene mutation was performed on formalin fixed paraffin embedded (FFPE) cancer patient tissue that was dissected using the mesodissection platform. Selected reaction monitoring proteomic analysis for quantitative Her2 protein expression was performed on FFPE patient tumour tissue dissected by a laser-based instrument and the MilliSect instrument.
  
  
  RESULTS
  Genomic analysis demonstrates highly confident detection of KRAS mutation specifically in lung cancer cells and not the surrounding benign, non-tumour tissue. Proteomic analysis demonstrates Her2 quantitative protein expression in breast cancer cells dissected manually, by laser-based instrumentation and by MilliSect instrumentation (mesodissection).
  
  
  CONCLUSIONS
  Slide-mounted tissue dissection is commonly performed using laser-based instruments or manually scraping tissue by scalpel. Here we demonstrate that the mesodissection platform as performed by the MilliSect instrument for tissue dissection is cost-effective; it functions comparably to laser-based dissection and which can be adopted into a clinical diagnostic workflow.
authors.affiliation: OncoPlex Diagnostics, Rockville, Maryland, USA.
authors.forename: David
authors.initials: D
authors.lastname: Krizman
authors.affiliation: AvanSci Bio, Salt Lake City, Utah, USA.
authors.forename: Nils
authors.initials: N
authors.lastname: Adey
authors.affiliation: AvanSci Bio, Salt Lake City, Utah, USA.
authors.forename: Robert
authors.initials: R
authors.lastname: Parry
mesh_terms: D057205:Automation, Laboratory; D001706:Biopsy; D001943:Breast Neoplasms / Q000737:chemistry* / Q000473:pathology; D004252:DNA Mutational Analysis; D004867:Equipment Design; D005260:Female; D005404:Fixatives; D005557:Formaldehyde; D006801:Humans; D061346:Laser Capture Microdissection / Q000295:instrumentation / Q000379:methods*; D008175:Lung Neoplasms / Q000235:genetics* / Q000473:pathology; D008297:Male; D025202:Molecular Diagnostic Techniques; D009154:Mutation; D016612:Paraffin Embedding; D011237:Predictive Value of Tests; D040901:Proteomics; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D016283:Proto-Oncogene Proteins p21(ras); D018719:Erb-b2 Receptor Tyrosine Kinases / Q000032:analysis*; D016707:Tissue Fixation; D057188:Workflow; D018631:ras Proteins / Q000235:genetics*
chemical_list: D005404:Fixatives; D005557:Formaldehyde; D011518:Proto-Oncogene Proteins; D016283:Proto-Oncogene Proteins p21(ras); D018719:Erb-b2 Receptor Tyrosine Kinases; D018631:ras Proteins; C117307:KRAS protein, human; C508053:ERBB2 protein, human
keywords: CANCER GENETICS; DIAGNOSTICS; PROTEINS