title: Antipyretic and antinociceptive potential of extract/fractions of Potentilla evestita and its isolated compound, acacetin.
abstract: BACKGROUND
  Fever and pain management is a very challenging job for the clinician as the available synthetic agents are causing serious side effects. The present research article deals with the antipyretic and antinociceptive activity of extract/fractions of Potentilla evestita and acacetin isolated from the chloroform fraction of the plant.
  
  
  METHODS
  Various chromatographic and spectroscopic techniques were used for the isolation and characterizion of compound. In-vivo yeast induced fibrile mice were used for antipyretic activity while acetic acid induced writhing and formalin tests were used for antinociceptive.
  
  
  RESULTS
  The extract/fractions of P. evestita caused marked antipyretic effect during various assessment times in which chloroform was the most prominent followed by ethyl acetate. When acacetin was injected, it produced marked effect with maximum activity of 33.28% and 55.01% at 5 and 10 mg/kg i.p respectively. When studied in acetic acid induced writhing test, the extract/fractions evoked significant antinociceptive effect in which chloroform was the most effective fraction followed by ethyl acetate. Acacetin showed significant antinociceptive effect with 44.77% and 67.03% reduction in abdominal constriction at 5 and 10 mg/kg i.p., respectively. Similarly, it evoked significant dose dependent reduction in noxious stimulation with 42.07% and 64.57% pain attenuation at 5 and 10 mg/kg i.p., respectively in initial phase. In the late phase, it illustrated more dominant effect with 46.32% and 67.29% reduction of painful sensation.
  
  
  CONCLUSIONS
  In conclusion, the extract/fractions of P. evestita as well as the isolated compound, acacetin showed strong antipyretic and antinociceptive activity in various animal models possibly mediated through both peripheral and central mechanism.
authors.forename: Abdur
authors.initials: A
authors.lastname: Rauf
authors.forename: Rehan
authors.initials: R
authors.lastname: Khan
authors.affiliation: Department of Pharmacy, Abdul Wali Khan University, Mardan 23200, Pakistan. hkdr2006@gmail.com.
authors.forename: Haroon
authors.initials: H
authors.lastname: Khan
authors.forename: Barkat
authors.initials: B
authors.lastname: Ullah
authors.forename: Samreen
authors.initials: S
authors.lastname: Pervez
mesh_terms: D019342:Acetic Acid; D000700:Analgesics / Q000494:pharmacology / Q000627:therapeutic use*; D000818:Animals; D058633:Antipyretics / Q000494:pharmacology / Q000627:therapeutic use*; D004195:Disease Models, Animal; D005334:Fever / Q000188:drug therapy*; D047309:Flavones / Q000494:pharmacology / Q000627:therapeutic use*; D008297:Male; D008807:Mice, Inbred BALB C; D010146:Pain / Q000188:drug therapy*; D010147:Pain Measurement; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use*; D018515:Plant Leaves; D031987:Potentilla / Q000737:chemistry*; D017208:Rats, Wistar
chemical_list: D000700:Analgesics; D058633:Antipyretics; D047309:Flavones; D010936:Plant Extracts; C023717:acacetin; D019342:Acetic Acid