title: 4-Aminophenyl boronic acid modified gold platforms for influenza diagnosis.
abstract: As a potential pandemic threat to human health, there has been an urgent need for rapid, sensitive, simpler and less expensive detection method for the highly pathogenic influenza A virus. For this purpose, Quartz Crystal Microbalance (QCM) and Surface Plasmon Resonance (SPR) sensors have been developed for the recognition of hemagglutinin (HA) which is a major protein of influenza A virus. 4-Aminophenyl boronic acid (4-APBA) has been synthesized and used as a new ligand for binding of sialic acid (SA) via boronic acid-sugar interaction. SA has an important role in binding of HA. QCM and SPR sensor surfaces have been modified with thiol groups and then 4-APBA and SA have been immobilized on sensor surfaces, respectively. Sensor surfaces have been screened with AFM and used for the determination of HA from aqueous solution. The selective recognition of the QCM and SPR sensors toward Concanavalin A has been reported in this work. Also, the binding capacity and detection limits of QCM and SPR sensors have been calculated and detection limits were found to be 4.7 × 10(-2) μM, (0.26 μg ml(-1)) and 1.28 × 10(-1) μM, (0.72 μg ml(-1)) in the 95% confidence interval, respectively.
authors.affiliation: Department of Chemistry, Anadolu University, Eskişehir, Turkey. Electronic address: semir@anadolu.edu.tr.
authors.forename: Sibel Emir
authors.initials: SE
authors.lastname: Diltemiz
authors.affiliation: Department of Chemistry, Anadolu University, Eskişehir, Turkey.
authors.forename: Arzu
authors.initials: A
authors.lastname: Ersöz
authors.affiliation: Department of Chemistry, Anadolu University, Eskişehir, Turkey.
authors.forename: Deniz
authors.initials: D
authors.lastname: Hür
authors.affiliation: Department of Chemistry, Anadolu University, Eskişehir, Turkey.
authors.forename: Rüstem
authors.initials: R
authors.lastname: Keçili
authors.affiliation: Department of Chemistry, Anadolu University, Eskişehir, Turkey.
authors.forename: Ridvan
authors.initials: R
authors.lastname: Say
mesh_terms: D000814:Aniline Compounds / Q000138:chemical synthesis / Q000737:chemistry*; D001897:Boronic Acids / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism; D006046:Gold / Q000737:chemistry*; D006388:Hemagglutinins / Q000032:analysis; D006801:Humans; D007251:Influenza, Human / Q000175:diagnosis*; D018625:Microscopy, Atomic Force; D019158:N-Acetylneuraminic Acid / Q000737:chemistry / Q000378:metabolism; D009975:Orthomyxoviridae / Q000378:metabolism; D058525:Quartz Crystal Microbalance Techniques; D020349:Surface Plasmon Resonance; D013499:Surface Properties
chemical_list: C000599649:4-aminophenylboronic acid; D000814:Aniline Compounds; D001897:Boronic Acids; D006388:Hemagglutinins; D006046:Gold; D019158:N-Acetylneuraminic Acid