title: Effect of tenascin-C on the repair of full-thickness osteochondral defects of articular cartilage in rabbits.
abstract: The purpose of this study was to examine the effect of tenascin-C (TNC) on the repair of full-thickness osteochondral defects of articular cartilage in vivo. We used a gellan-gellan-sulfate sponge (Gellan-GS) to maintain a TNC-rich environment in the cartilage defects. We implanted Gellan-GS soaked in PBS only (Group 1), Gellan-GS soaked in 10 µg/ml of TNC (Group 2), and Gellan-GS soaked in 100 µg/ml of TNC (Group 3) into a full-thickness osteochondral defect of the patellar groove of rabbits. The defect area was examined grossly and histologically 4-12 weeks after surgery. Sections of synovium were also immunohistochemically investigated. Histologically as well as macroscopically, the defects in Group 2 showed better repair than the other groups at 8 and 12 weeks after surgery. Inflammation of the synovium tended to diminish over time in all groups, and the degree of synovitis was the same for all three groups at each time point. In conclusion, Gellan-GS soaked in TNC can be used as a novel scaffold for the repair of articular cartilage defects. This study also indicates that TNC promotes the repair of full-thickness osteochondral defects in vivo.
authors.affiliation: Department of Orthopaedic Surgery, Mie University Graduate School of Medicine, Mie, Japan.
authors.forename: Shigeto
authors.initials: S
authors.lastname: Ikemura
authors.forename: Masahiro
authors.initials: M
authors.lastname: Hasegawa
authors.forename: Takahiro
authors.initials: T
authors.lastname: Iino
authors.forename: Keiichi
authors.initials: K
authors.lastname: Miyamoto
authors.forename: Kyoko
authors.initials: K
authors.lastname: Imanaka-Yoshida
authors.forename: Toshimichi
authors.initials: T
authors.lastname: Yoshida
authors.forename: Akihiro
authors.initials: A
authors.lastname: Sudo
mesh_terms: D000818:Animals; D002358:Cartilage, Articular / Q000187:drug effects* / Q000293:injuries* / Q000473:pathology; D003692:Delayed-Action Preparations; D004195:Disease Models, Animal; D005260:Female; D010329:Patella / Q000293:injuries; D011134:Polysaccharides / Q000494:pharmacology; D011135:Polysaccharides, Bacterial / Q000494:pharmacology; D011817:Rabbits; D013463:Sulfuric Acid Esters / Q000494:pharmacology; D015503:Surgical Sponges; D013583:Synovial Membrane / Q000473:pathology; D019063:Tenascin / Q000494:pharmacology*; D014945:Wound Healing / Q000187:drug effects*; D014950:Wounds, Penetrating / Q000188:drug therapy* / Q000473:pathology / Q000503:physiopathology
chemical_list: D003692:Delayed-Action Preparations; D011134:Polysaccharides; D011135:Polysaccharides, Bacterial; D013463:Sulfuric Acid Esters; D019063:Tenascin; C470030:gellan sulfate; C048288:gellan gum
keywords: animal model; cartilage repair; gellan-gellan-sulfate; tenascin-C