title: Nanofibrous nonmulberry silk/PVA scaffold for osteoinduction and osseointegration.
abstract: Poly-vinyl alcohol and nonmulberry tasar silk fibroin of Antheraea mylitta are blended to fabricate nanofibrous scaffolds for bone regeneration. Nanofibrous matrices are prepared by electrospinning the equal volume ratio blends of silk fibroin (2 and 4 wt%) with poly-vinyl alcohol solution (10 wt%) and designated as 2SF/PVA and 4SF/PVA, respectively with average nanofiber diameters of 177 ± 13 nm (2SF/PVA) and 193 ± 17 nm (4SF/PVA). Fourier transform infrared spectroscopy confirms retention of the secondary structure of fibroin in blends indicating the structural stability of neo-matrix. Both thermal stability and contact angle of the blends decrease with increasing fibroin percentage. Conversely, fibroin imparts mechanical stability to the blends; greater tensile strength is observed with increasing fibroin concentration. Blended scaffolds are biodegradable and support well the neo-bone matrix synthesis by human osteoblast like cells. The findings indicate the potentiality of nanofibrous scaffolds of nonmulberry fibroin as bone scaffolding material.
authors.affiliation: Materials Science Centre, Indian Institute of Technology Kharagpur, Kharagpur, 721302, India.
authors.forename: Promita
authors.initials: P
authors.lastname: Bhattacharjee
authors.forename: Banani
authors.initials: B
authors.lastname: Kundu
authors.forename: Deboki
authors.initials: D
authors.lastname: Naskar
authors.forename: Tapas K
authors.initials: TK
authors.lastname: Maiti
authors.forename: Debasis
authors.initials: D
authors.lastname: Bhattacharya
authors.forename: Subhas C
authors.initials: SC
authors.lastname: Kundu
mesh_terms: D000438:Alcohols / Q000737:chemistry; D001842:Bone and Bones / Q000166:cytology*; D005349:Fibroins / Q000737:chemistry*; D057139:Nanofibers / Q000737:chemistry*; D047011:Silk / Q000737:chemistry*; D013718:Tensile Strength; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D000438:Alcohols; D047011:Silk; D005349:Fibroins
keywords: bone tissue engineering; electrospinning; nanofibers; nonmulberry silk fibroin; poly-vinyl alcohol