title: Poly (hydroxyethyl methacrylate-glycidyl methacrylate) films modified with different functional groups: In vitro interactions with platelets and rat stem cells.
abstract: Copolymerization of 2-hydroxyethylmethacrylate (HEMA) with glycidylmethacrylate (GMA) in the presence of α-α'-azoisobisbutyronitrile (AIBN) resulted in the formation of hydrogel films carrying reactive epoxy groups. Thirteen kinds of different molecules with pendant NH2 group were used for modifications of the p(HEMA-GMA) films. The NH2 group served as anchor binding site for immobilization of functional groups on the hydrogel film via direct epoxy ring opening reaction. The modified hydrogel films were characterized by FTIR, and contact angle studies. In addition, mechanical properties of the hydrogel films were studied, and modified hydrogel films showed improved mechanical properties compared with the non-modified film, but they are less elastic than the non-modified film. The biological activities of these films such as platelet adhesion, red blood cells hemolysis, and swelling behavior were studied. The effect of modified hydrogel films, including NH2, (using different aliphatic CH2 chain lengths) CH3, SO3H, aromatic groups with substituted OH and COOH groups, and amino acids were also investigated on the adhesion, morphology and survival of rat mesenchymal stem cells (MSCs). The MTT colorimetric assay reveals that the p(HEMA-GMA)-GA-AB, p(HEMA-GMA)-GA-Phe, p(HEMA-GMA)-GA-Trp, p(HEMA-GMA)-GA-Glu formulations have an excellent biocompatibility to promote the cell adhesion and growth. We anticipate that the fabricated p(HEMA-GMA) based hydrogel films with controllable surface chemistry and good stable swelling ratio may find extensive applications in future development of tissue engineering scaffold materials, and in various biotechnological areas.
authors.affiliation: Biochemical Processing and Biomaterial Research Laboratory, Faculty of Sciences, Gazi University, 06500-Teknikokullar-Ankara, Turkey. Electronic address: g_bayramoglu@hotmail.com.
authors.forename: Gulay
authors.initials: G
authors.lastname: Bayramoglu
authors.affiliation: Laboratory of Stem Cell Research, Department of Molecular Biology and Genetics, Faculty Science, Bilkent University, 06800 Bilkent-Ankara, Turkey.
authors.forename: Verda
authors.initials: V
authors.lastname: Bitirim
authors.affiliation: Department of Pharmaceutical Microbiology, Faculty Pharmacy, Anadolu University, Yunus Emre Campus, Eskisehir, Turkey.
authors.forename: Yagmur
authors.initials: Y
authors.lastname: Tunali
authors.affiliation: Biochemical Processing and Biomaterial Research Laboratory, Faculty of Sciences, Gazi University, 06500-Teknikokullar-Ankara, Turkey.
authors.forename: Mehmet Yakup
authors.initials: MY
authors.lastname: Arica
authors.affiliation: Laboratory of Stem Cell Research, Department of Molecular Biology and Genetics, Faculty Science, Bilkent University, 06800 Bilkent-Ankara, Turkey.
authors.forename: Kamil Can
authors.initials: KC
authors.lastname: Akcali
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology; D001792:Blood Platelets / Q000166:cytology*; D002448:Cell Adhesion / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D005260:Female; D006461:Hemolysis / Q000187:drug effects; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D011102:Polyhydroxyethyl Methacrylate / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D013499:Surface Properties; D013718:Tensile Strength; D023822:Tissue Engineering; D054457:Tissue Scaffolds
chemical_list: D001672:Biocompatible Materials; C515720:poly(hydroxyethyl methacrylate-co-glycerol methacrylate); D011102:Polyhydroxyethyl Methacrylate; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate