title: Increased adipogenesis of human adipose-derived stem cells on polycaprolactone fiber matrices.
abstract: With accelerating rates of obesity and type 2 diabetes world-wide, interest in studying the adipocyte and adipose tissue is increasing. Human adipose derived stem cells--differentiated to adipocytes in vitro--are frequently used as a model system for white adipocytes, as most of their pathways and functions resemble mature adipocytes in vivo. However, these cells are not completely like in vivo mature adipocytes. Hosting the cells in a more physiologically relevant environment compared to conventional two-dimensional cell culturing on plastic surfaces, can produce spatial cues that drive the cells towards a more mature state. We investigated the adipogenesis of adipose derived stem cells on electro spun polycaprolactone matrices and compared functionality to conventional two-dimensional cultures as well as to human primary mature adipocytes. To assess the degree of adipogenesis we measured cellular glucose-uptake and lipolysis and used a range of different methods to evaluate lipid accumulation. We compared the averaged results from a whole population with the single cell characteristics--studied by coherent anti-Stokes Raman scattering microscopy--to gain a comprehensive picture of the cell phenotypes. In adipose derived stem cells differentiated on a polycaprolactone-fiber matrix; an increased sensitivity in insulin-stimulated glucose uptake was detected when cells were grown on either aligned or random matrices. Furthermore, comparing differentiation of adipose derived stem cells on aligned polycaprolactone-fiber matrixes, to those differentiated in two-dimensional cultures showed, an increase in the cellular lipid accumulation, and hormone sensitive lipase content. In conclusion, we propose an adipocyte cell model created by differentiation of adipose derived stem cells on aligned polycaprolactone-fiber matrices which demonstrates increased maturity, compared to 2D cultured cells.
authors.affiliation: Reagent and Assay Development Discovery Sciences R&D, Astra Zeneca, Mölndal, Sweden.
authors.forename: Cecilia
authors.initials: C
authors.lastname: Brännmark
authors.affiliation: Molecular Microscopy Department of Chemical and Biological Engineering, Chalmers University of Technology, Gothenburg, Sweden.
authors.forename: Alexandra
authors.initials: A
authors.lastname: Paul
authors.affiliation: Reagent and Assay Development Discovery Sciences R&D, Astra Zeneca, Mölndal, Sweden; Molecular Microscopy Department of Chemical and Biological Engineering, Chalmers University of Technology, Gothenburg, Sweden.
authors.forename: Diana
authors.initials: D
authors.lastname: Ribeiro
authors.affiliation: Reagent and Assay Development Discovery Sciences R&D, Astra Zeneca, Mölndal, Sweden.
authors.forename: Björn
authors.initials: B
authors.lastname: Magnusson
authors.affiliation: Reagent and Assay Development Discovery Sciences R&D, Astra Zeneca, Mölndal, Sweden.
authors.forename: Gabriella
authors.initials: G
authors.lastname: Brolén
authors.affiliation: Molecular Microscopy Department of Chemical and Biological Engineering, Chalmers University of Technology, Gothenburg, Sweden.
authors.forename: Annika
authors.initials: A
authors.lastname: Enejder
authors.affiliation: Reagent and Assay Development Discovery Sciences R&D, Astra Zeneca, Mölndal, Sweden.
authors.forename: Anna
authors.initials: A
authors.lastname: Forslöw
mesh_terms: D017667:Adipocytes / Q000166:cytology / Q000378:metabolism; D050156:Adipogenesis; D000273:Adipose Tissue / Q000166:cytology* / Q000378:metabolism; D000328:Adult; D018929:Cell Culture Techniques / Q000379:methods; D002454:Cell Differentiation / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics; D002478:Cells, Cultured; D005260:Female; D015870:Gene Expression; D005947:Glucose / Q000378:metabolism / Q000493:pharmacokinetics; D006801:Humans; D008055:Lipids / Q000032:analysis; D008066:Lipolysis; D008297:Male; D036641:Microscopy, Fluorescence, Multiphoton; D011091:Polyesters; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013059:Spectrum Analysis, Raman / Q000379:methods; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism; D054457:Tissue Scaffolds / Q000737:chemistry; D055815:Young Adult
chemical_list: D008055:Lipids; D011091:Polyesters; C016240:polycaprolactone; D005947:Glucose