title: Transplantation of Cerebral Dopamine Neurotrophic Factor Transducted BMSCs in Contusion Spinal Cord Injury of Rats: Promotion of Nerve Regeneration by Alleviating Neuroinflammation.
abstract: Traumatic spinal cord injury (SCI) causes neuron death and axonal damage resulting in functional motor and sensory loss, showing limited regeneration because of adverse microenvironment such as neuroinflammation and glial scarring. Currently, there is no effective therapy to treat SCI in clinical practice. Bone marrow-derived mesenchymal stem cells (BMSCs) are candidates for cell therapies but its effect is limited by neuroinflammation and adverse microenvironment in the injured spinal cord. In this study, we developed transgenic BMSCs overexpressing cerebral dopamine neurotrophic factor (CDNF), a secretory neurotrophic factor that showed potent effects on neuron protection, anti-inflammation, and sciatic nerve regeneration in previous studies. Our results showed that the transplantation of CDNF-BMSCs suppressed neuroinflammation and decreased the production of proinflammatory cytokines after SCI, resulting in the promotion of locomotor function and nerve regeneration of the injured spinal cord. This study presents a novel promising strategy for the treatment of spinal cord injury.
authors.affiliation: Department of Spine Surgery, Qilu Hospital of Shandong University, 250012, Jinan, China.|Shandong University Qilu Hospital Research Center for Cell Therapy, Key Laboratory of Cardiovascular Remodeling and Function Research, Qilu Hospital of Shandong University, Jinan, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Zhao
authors.affiliation: Department of Spine Surgery, Qilu Hospital of Shandong University, 250012, Jinan, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Cheng
authors.affiliation: Department of Pediatric Surgery, Laizhou People's Hospital, Laizhou, China.
authors.forename: Xinwen
authors.initials: X
authors.lastname: Du
authors.affiliation: Department of Spine Surgery, Qilu Hospital of Shandong University, 250012, Jinan, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Hou
authors.affiliation: Department of Spine Surgery, Qilu Hospital of Shandong University, 250012, Jinan, China.|Shandong University Qilu Hospital Research Center for Cell Therapy, Key Laboratory of Cardiovascular Remodeling and Function Research, Qilu Hospital of Shandong University, Jinan, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Shandong University Qilu Hospital Research Center for Cell Therapy, Key Laboratory of Cardiovascular Remodeling and Function Research, Qilu Hospital of Shandong University, Jinan, China.
authors.forename: Zhaoqiang
authors.initials: Z
authors.lastname: Cui
authors.affiliation: Department of Spine Surgery, Qilu Hospital of Shandong University, 250012, Jinan, China. niemedmail@126.com.
authors.forename: Lin
authors.initials: L
authors.lastname: Nie
mesh_terms: D000818:Animals; D001369:Axons / Q000502:physiology; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology; D064987:Cell- and Tissue-Based Therapy; D004195:Disease Models, Animal; D004298:Dopamine / Q000378:metabolism*; D005260:Female; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D009416:Nerve Regeneration / Q000187:drug effects* / Q000502:physiology; D017208:Rats, Wistar; D020127:Recovery of Function / Q000502:physiology*; D013119:Spinal Cord Injuries / Q000503:physiopathology / Q000628:therapy*
chemical_list: D004298:Dopamine
keywords: Bone marrow-derived mesenchymal stem cells; Cell therapy; Cerebral dopamine neurotrophic factor; Nerve regeneration; Neuroinflammation; Spinal cord injury