title: Complete genome sequence of a clinical Bordetella pertussis isolate from Brazil.
abstract: There has been a resurgence in the number of pertussis cases in Brazil and around the world. Here, the genome of a clinical Bordetella pertussis strain (Bz181) that was recently isolated in Brazil is reported. Analysis of the virulence-associated genes defining the pre- and post-vaccination lineages revealed the presence of the prn2-ptxS1A-fim3B-ptxP3 allelic profile in Bz181, which is characteristic of the current pandemic lineage. A putative metallo-β-lactamase gene presenting all of the conserved zinc-binding motifs that characterise the catalytic site was identified, in addition to a multidrug efflux pump of the RND family that could confer resistance to erythromycin, which is the antibiotic of choice for treating pertussis disease.
authors.affiliation: Laboratório de Genética Molecular de Microorganismos, Instituto Oswaldo Cruz-Fiocruz, Rio de Janeiro, RJ, Brasil.
authors.forename: Bruno Gabriel N
authors.initials: BG
authors.lastname: Andrade
authors.affiliation: Laboratório de Genética Molecular de Microorganismos, Instituto Oswaldo Cruz-Fiocruz, Rio de Janeiro, RJ, Brasil.
authors.forename: Michel F Abanto
authors.initials: MF
authors.lastname: Marin
authors.affiliation: Laboratório de Genética Molecular de Microorganismos, Instituto Oswaldo Cruz-Fiocruz, Rio de Janeiro, RJ, Brasil.
authors.forename: Diego Duque
authors.initials: DD
authors.lastname: Cambuy
authors.affiliation: Laboratório de Genética Molecular de Microorganismos, Instituto Oswaldo Cruz-Fiocruz, Rio de Janeiro, RJ, Brasil.
authors.forename: Erica Lourenço
authors.initials: EL
authors.lastname: Fonseca
authors.affiliation: Laboratório Central de Saúde Pública Dr Milton Sobral, Recife, PE, Brasil.
authors.forename: Nadjla Ferreira
authors.initials: NF
authors.lastname: Souza
authors.affiliation: Laboratório de Genética Molecular de Microorganismos, Instituto Oswaldo Cruz-Fiocruz, Rio de Janeiro, RJ, Brasil.
authors.forename: Ana Carolina P
authors.initials: AC
authors.lastname: Vicente
mesh_terms: D000483:Alleles; D001886:Bordetella pertussis / Q000145:classification / Q000235:genetics* / Q000472:pathogenicity; D001938:Brazil; D016680:Genome, Bacterial; D006801:Humans; D010566:Virulence Factors, Bordetella / Q000235:genetics*; D014917:Whooping Cough / Q000235:genetics / Q000382:microbiology*
chemical_list: D010566:Virulence Factors, Bordetella