title: A highly diverse spectrum of naphthoquinone derivatives produced by the endophytic fungus Biatriospora sp. CCF 4378.
abstract: A strain of Biatriospora sp. CCF 4378 was tested for the production of secondary metabolites under submerged fermentation conditions. Eleven compounds were isolated from the culture broth, and the structures of these compounds were determined using HRMS, NMR and X-ray analysis. In addition to six known naphthoquinone derivatives, i.e. ascomycone A, ascomycone B, 6-deoxyfusarubine, 6-deoxyanhydrofusarubine, herbarine and balticol A, one derivative of 2-azaanthraquinone, 6-deoxybostrycoidine, was also identified. Four new natural pyranonaphthoquinones were found, and these natural products were pleorubrin A, pleorubrin B, pleorubrin C and pleorubrin D. The toxicity on human cell lines of the crude naphthoquinone fraction and pure 6-deoxybostrycoidin, ascomycone B, pleorubrin B and 6-deoxyfusarubin was tested. Ascomycone B and 6-deoxyfusarubin elicited rapid cytotoxicity at micromolar concentrations.
authors.affiliation: Institute of Microbiology of the ASCR, v.v.i., Vídeňská 1083, 142 20, Prague 4, Czech Republic.
authors.forename: Eva
authors.initials: E
authors.lastname: Stodůlková
authors.forename: Petr
authors.initials: P
authors.lastname: Man
authors.forename: Marek
authors.initials: M
authors.lastname: Kuzma
authors.forename: Jan
authors.initials: J
authors.lastname: Černý
authors.forename: Ivana
authors.initials: I
authors.lastname: Císařová
authors.forename: Alena
authors.initials: A
authors.lastname: Kubátová
authors.forename: Milada
authors.initials: M
authors.lastname: Chudíčková
authors.forename: Miroslav
authors.initials: M
authors.lastname: Kolařík
authors.forename: Miroslav
authors.initials: M
authors.lastname: Flieger
mesh_terms: D001203:Ascomycota / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D060026:Endophytes / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D006801:Humans; D015394:Molecular Structure; D009285:Naphthoquinones / Q000737:chemistry* / Q000378:metabolism* / Q000494:pharmacology; D034403:Ulmus / Q000382:microbiology*
chemical_list: D009285:Naphthoquinones