title: A time-resolved luminescence biosensor assay for anaplastic lymphoma kinase (ALK) activity.
abstract: A novel time-resolved luminescence biosensor assay for anaplastic lymphoma kinase (ALK) was developed. We used a straightforward strategy to modify a known ALK substrate into a peptide biosensor that can accommodate terbium luminescence sensitization upon its phosphorylation by ALK. Since this strategy is generalizable, this high-throughput screening compatible assay serves as an example for development of other kinase assays that employ terbium luminescence as a read-out.
authors.affiliation: Department of Medicinal Chemistry and Molecular Pharmacology, College of Pharmacy, Purdue Center for Cancer Research, Purdue University, West Lafayette, IN 47907, USA. llparker@umn.edu.
authors.forename: Wei
authors.initials: W
authors.lastname: Cui
authors.forename: Laurie L
authors.initials: LL
authors.lastname: Parker
mesh_terms: D000595:Amino Acid Sequence; D000077548:Anaplastic Lymphoma Kinase; D015374:Biosensing Techniques / Q000379:methods; D057075:Enzyme Assays / Q000379:methods; D049449:Luminescence; D008163:Luminescent Measurements / Q000379:methods*; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry / Q000378:metabolism; D020794:Receptor Protein-Tyrosine Kinases / Q000737:chemistry / Q000378:metabolism*; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D013725:Terbium / Q000032:analysis / Q000378:metabolism
chemical_list: D010455:Peptides; D011994:Recombinant Proteins; D013725:Terbium; D000077548:Anaplastic Lymphoma Kinase; D020794:Receptor Protein-Tyrosine Kinases