title: Bovine milk fermented with Lactobacillus rhamnosus L8020 decreases the oral carriage of mutans streptococci and the burden of periodontal pathogens.
abstract: OBJECTIVE
  The aim of this study was to find the oral isolate of lactobacilli, which has the potential to inhibit either periodontal, cariogenic, or fungal pathogens in vitro, and to examine the effects of bovine milk fermented with the isolate on the oral carriage of cariogenic and periodontal pathogens.
  
  
  METHODS
  The inhibitory effects of the supernatant of Man-Rogosa-Sharpe broth, in which each of 42 oral isolates of lactobacilli grown, was examined. One isolate, Lactobacillus rhamnosus L8020, that showed the potential to inhibit either periodontal, cariogenic, or fungal pathogens in vitro, was used to examine the effects of fermented milk on the oral carriage of cariogenic and periodontal pathogens, which was examined by a placebo-controlled and cohort trial using 50 participants.
  
  
  RESULTS
  Edible yogurt containing Lactobacillus rhamnosus L8020 significantly reduced the oral carriage of mutans streptococci (P < 0.01) and four periodontal pathogens examined: Porphyromonas gingivalis, Prevotella intermedia, Tannerella forsythia, and Fusobacterium spp. (P < 0.01), but the phenomenon were not observed with the placebo yogurt (P > 0.05).
  
  
  CONCLUSIONS
  These results suggest that yogurt with Lactobacillus rhamnosus L8020 could reduce the risk of dental caries and periodontal disease.
authors.affiliation: Department of Oral Biology and Engineering, Hiroshima University, Hiroshima, Japan Department of Prosthodontics, Nagasaki University, Nagasaki, Japan.
authors.forename: Hiroki
authors.initials: H
authors.lastname: Nikawa
authors.forename: Yu
authors.initials: Y
authors.lastname: Tomiyama
authors.forename: Minako
authors.initials: M
authors.lastname: Hiramatsu
authors.forename: Kaori
authors.initials: K
authors.lastname: Yushita
authors.forename: Yuko
authors.initials: Y
authors.lastname: Takamoto
authors.forename: Hitomi
authors.initials: H
authors.lastname: Ishi
authors.forename: Sumiyo
authors.initials: S
authors.lastname: Mimura
authors.forename: Aya
authors.initials: A
authors.lastname: Hiyama
authors.forename: Hisako
authors.initials: H
authors.lastname: Sasahara
authors.forename: Kazuko
authors.initials: K
authors.lastname: Kawahara
authors.forename: Seicho
authors.initials: S
authors.lastname: Makihira
authors.forename: Takahiro
authors.initials: T
authors.lastname: Satoda
authors.forename: Toshinobu
authors.initials: T
authors.lastname: Takemoto
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Murata
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Mine
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Taji
mesh_terms: D000818:Animals; D000898:Antibiosis / Q000502:physiology*; D058491:Bacterial Load; D001431:Bacteriological Techniques; D001439:Bacteroides / Q000502:physiology; D002176:Candida albicans / Q000502:physiology; D002417:Cattle; D015331:Cohort Studies; D004311:Double-Blind Method; D005260:Female; D005673:Fusobacterium / Q000502:physiology; D006090:Gram-Negative Bacteria / Q000502:physiology*; D006801:Humans; D052201:Lacticaseibacillus rhamnosus / Q000378:metabolism* / Q000502:physiology; D008297:Male; D009055:Mouth / Q000382:microbiology*; D010919:Placebos; D016966:Porphyromonas gingivalis / Q000502:physiology; D018713:Prevotella intermedia / Q000502:physiology; D012463:Saliva / Q000382:microbiology; D013295:Streptococcus mutans / Q000502:physiology*; D016986:Streptococcus sobrinus / Q000502:physiology; D015014:Yogurt / Q000382:microbiology*; D055815:Young Adult
chemical_list: D010919:Placebos
keywords: Lactobacillus rhamnosus; dental caries; mutans streptococci; periodontal disease yogurt