title: Investigation of the role of 8-OHdG and oxidative stress in papillary thyroid carcinoma.
abstract: The aim of this study was to determine levels of serum 8-hydroxy-2'-deoxyguanosine (8-OHdG) as an indicator of oxidant-induced DNA damage and oxidant status in patients with papillary thyroid carcinoma before and after surgery. This study included 25 patients with papillary thyroid carcinoma and age-matched 27 healthy controls. Total antioxidant status (TAS), total oxidant status (TOS), lipid hydroperoxide (LOOH), and 8-OHdG levels were measured. 8-OHdG levels were significantly higher in the preoperative papillary thyroid carcinoma (PTC) group compared with the healthy control group (p < 0.001) and were significantly lower after operation in patients with papillary thyroid carcinoma (p = 0.004). Oxidative stress index (OSI) levels were significantly higher in both preoperative and postoperative PTC patients compared with the healthy control group (p < 0.001 and p < 0.001, respectively). TOS levels were higher in the preoperative and postoperative PTC groups compared to the healthy control group (p < 0.001 and p < 0.001, respectively). TAS levels was lower in the preoperative PTC groups compared to the healthy control group (p = 0.011). Serum LOOH levels were higher in both preoperative and postoperative PTC groups compared to the healthy control group (p < 0.001 and p < 0.001, respectively). Correlation analysis yielded that serum 8-OHdG levels were positively correlated with OSİ and LOOH levels in patients with PTC before surgery (r = 0.668, p < 0.001; r = 0.446, p = 0.025, respectively) and had a negative correlation with TAS levels (r = -0.616, p = 0.001). We have shown severe oxidative DNA damage and impaired antioxidant status in papillary thyroid carcinoma.
authors.affiliation: Department of Endocrinology, Faculty of Medicine, Gaziantep University, Gaziantep, Turkey, suzan2471@yahoo.com.tr.
authors.forename: Suzan
authors.initials: S
authors.lastname: Tabur
authors.forename: Şefika Nur
authors.initials: ŞN
authors.lastname: Aksoy
authors.forename: Hakan
authors.initials: H
authors.lastname: Korkmaz
authors.forename: Mesut
authors.initials: M
authors.lastname: Ozkaya
authors.forename: Nurten
authors.initials: N
authors.lastname: Aksoy
authors.forename: Ersin
authors.initials: E
authors.lastname: Akarsu
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D000328:Adult; D000975:Antioxidants / Q000008:administration & dosage*; D002265:Carboxylic Ester Hydrolases / Q000235:genetics; D002277:Carcinoma / Q000188:drug therapy* / Q000235:genetics* / Q000473:pathology; D002291:Carcinoma, Papillary; D004249:DNA Damage / Q000187:drug effects; D003849:Deoxyguanosine / Q000008:administration & dosage / Q000031:analogs & derivatives; D005260:Female; D006801:Humans; D008054:Lipid Peroxides / Q000008:administration & dosage; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress / Q000187:drug effects*; D000077273:Thyroid Cancer, Papillary; D013964:Thyroid Neoplasms / Q000188:drug therapy* / Q000235:genetics* / Q000473:pathology
chemical_list: D000975:Antioxidants; D008054:Lipid Peroxides; D000080242:8-Hydroxy-2'-Deoxyguanosine; D002265:Carboxylic Ester Hydrolases; D003849:Deoxyguanosine