title: Efficiency of the inbreeding coefficient f and other estimators in detecting null alleles, as revealed by empirical data of locus oke3 across 65 populations of chum salmon Oncorhynchus keta.
abstract: A survey of 65 populations of chum salmon Oncorhynchus keta across the species range revealed homozygote excess (947 homozygotes in 2954 fish) at a polymerase chain reaction (PCR)-based simple sequence repeat (SSR) locus oke3 with multiple alleles, whereas re-designed PCR primers indicated that 328 of these homozygotes were actually heterozygotes. Statistically significant high positive values of inbreeding coefficients, f, in multiple populations appeared to be a reliable predictor of null alleles. Based on these data, three methods were checked for their ability to estimate null-allele frequencies.
authors.affiliation: N.I. Vavilov Institute of General Genetics, 3 Gubkin St, Moscow, 119991, Russia.
authors.forename: L A
authors.initials: LA
authors.lastname: Zhivotovsky
authors.forename: S Yu
authors.initials: SY
authors.lastname: Kordicheva
authors.forename: E G
authors.initials: EG
authors.lastname: Shaikhaev
authors.forename: G A
authors.initials: GA
authors.lastname: Rubtsova
authors.forename: K I
authors.initials: KI
authors.lastname: Afanasiev
authors.forename: M V
authors.initials: MV
authors.lastname: Shitova
authors.forename: S A
authors.initials: SA
authors.lastname: Fuller
authors.forename: G O
authors.initials: GO
authors.lastname: Shaikhaev
authors.forename: A J
authors.initials: AJ
authors.lastname: Gharrett
mesh_terms: D000483:Alleles; D000818:Animals; D017931:DNA Primers; D005787:Gene Frequency; D005828:Genetics, Population; D006579:Heterozygote; D006720:Homozygote; D007178:Inbreeding; D018895:Microsatellite Repeats; D017685:Oncorhynchus keta / Q000235:genetics*; D016133:Polymerase Chain Reaction
chemical_list: D017931:DNA Primers
keywords: DNA amplification; SNP; f-value; null allele; population study