title: Oxidative stress contributes to orthopedic trauma-induced acute kidney injury in obese rats.
abstract: After trauma, obese patients have an increased risk of developing acute kidney injury (AKI). We have demonstrated that obese Zucker (OZ) rats, but not lean Zucker (LZ) rats, develop AKI 24 h after orthopedic trauma. ROS have been implicated in the pathophysiology of AKI in models of critical illness. However, the contribution of ROS to trauma-induced AKI in the setting of obesity has not been determined. We hypothesized that AKI in OZ rats after trauma is mediated by increased oxidative stress. Male LZ and OZ rats were divided into control and trauma groups, with a subset receiving treatment after trauma with the antioxidant apocynin (50 mg/kg ip, 2 mM in drinking water). The day after trauma, glomerular filtration rate, plasma creatinine, urine kidney injury molecule-1, and albumin excretion as well as renal oxidant and antioxidant activity were measured. After trauma, compared with LZ rats, OZ rats exhibited a significant decrease in glomerular filtration rate along with significant increases in plasma creatinine and urine kidney injury molecule-1 and albumin excretion. Additionally, oxidative stress was significantly increased in OZ rats, as evidenced by increased renal NADPH oxidase activity and urine lipid peroxidation products (thiobarbituric acid-reactive substances), and OZ rats also had suppressed renal superoxide dismutase activity. Apocynin treatment significantly decreased oxidative stress and AKI in OZ rats but had minimal effects in LZ rats. These results suggest that ROS play an important role in AKI in OZ rats after traumatic injury and that ROS may be a potential future therapeutic target in the obese after trauma.
authors.affiliation: Department of Physiology and Biophysics, University of Mississippi Medical Center, Jackson, Mississippi.
authors.forename: Peter N
authors.initials: PN
authors.lastname: Mittwede
authors.affiliation: Department of Physiology and Biophysics, University of Mississippi Medical Center, Jackson, Mississippi.
authors.forename: Lusha
authors.initials: L
authors.lastname: Xiang
authors.affiliation: Department of Physiology and Biophysics, University of Mississippi Medical Center, Jackson, Mississippi.
authors.forename: Silu
authors.initials: S
authors.lastname: Lu
authors.affiliation: Department of Physiology and Biophysics, University of Mississippi Medical Center, Jackson, Mississippi.
authors.forename: John S
authors.initials: JS
authors.lastname: Clemmer
authors.affiliation: Department of Physiology and Biophysics, University of Mississippi Medical Center, Jackson, Mississippi Rhester@umc.edu.
authors.forename: Robert L
authors.initials: RL
authors.lastname: Hester
mesh_terms: D058186:Acute Kidney Injury / Q000209:etiology* / Q000378:metabolism; D000419:Albuminuria / Q000209:etiology; D000818:Animals; D001794:Blood Pressure; D001835:Body Weight; D015815:Cell Adhesion Molecules / Q000652:urine; D003404:Creatinine / Q000097:blood; D050723:Fractures, Bone / Q000150:complications* / Q000378:metabolism; D005919:Glomerular Filtration Rate; D008297:Male; D009765:Obesity / Q000378:metabolism*; D018384:Oxidative Stress; D011924:Rats, Zucker; D017382:Reactive Oxygen Species / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000097:blood
chemical_list: D015815:Cell Adhesion Molecules; D003404:Creatinine; D017382:Reactive Oxygen Species; D014409:Tumor Necrosis Factor-alpha; C110936:Havcr1 protein, rat
keywords: acute kidney injury; inflammation; obesity; reactive oxygen species; trauma