title: Three-dimensional conformal intensity-modulated radiation therapy of left femur foci does not damage the sciatic nerve.
abstract: During radiotherapy to kill femoral hydatid tapeworms, the sciatic nerve surrounding the focus can be easily damaged by the treatment. Thus, it is very important to evaluate the effects of radiotherapy on the surrounding nervous tissue. In the present study, we used three-dimensional, conformal, intensity-modulated radiation therapy to treat bilateral femoral hydatid disease in Meriones meridiani. The focus of the hydatid disease on the left femur was subjected to radiotherapy (40 Gy) for 14 days, and the right femur received sham irradiation. Hematoxylin-eosin staining, electron microscopy, and terminal deoxynucleotidyl transferase-dUTP nick end labeling assays on the left femurs showed that the left sciatic nerve cell structure was normal, with no obvious apoptosis after radiation. Trypan blue staining demonstrated that the overall protoscolex structure in bone parasitized with Echinococcus granulosus disappeared in the left femur of the animals after treatment. The mortality of the protoscolex was higher in the left side than in the right side. The succinate dehydrogenase activity in the protoscolex in bone parasitized with Echinococcus granulosus was lower in the left femur than in the right femur. These results suggest that three-dimensional conformal intensity-modulated radiation therapy achieves good therapeutic effects on the secondary bone in hydatid disease in Meriones meridiani without damaging the morphology or function of the sciatic nerve.
authors.affiliation: People's Hospital of Xinjiang Uygur Autonomous Region, Urumqi, Xinjiang Uygur Autonomous Region, China.
authors.forename: Wanlong
authors.initials: W
authors.lastname: Xu
authors.affiliation: People's Hospital of Xinjiang Uygur Autonomous Region, Urumqi, Xinjiang Uygur Autonomous Region, China.
authors.forename: Xibin
authors.initials: X
authors.lastname: Zhao
authors.affiliation: People's Hospital of Xinjiang Uygur Autonomous Region, Urumqi, Xinjiang Uygur Autonomous Region, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Wang
authors.affiliation: People's Hospital of Xinjiang Uygur Autonomous Region, Urumqi, Xinjiang Uygur Autonomous Region, China.
authors.forename: Jungang
authors.initials: J
authors.lastname: Sun
authors.affiliation: People's Hospital of Xinjiang Uygur Autonomous Region, Urumqi, Xinjiang Uygur Autonomous Region, China.
authors.forename: Jiangbo
authors.initials: J
authors.lastname: Xu
authors.affiliation: People's Hospital of Xinjiang Uygur Autonomous Region, Urumqi, Xinjiang Uygur Autonomous Region, China.
authors.forename: Wenzheng
authors.initials: W
authors.lastname: Zhou
authors.affiliation: People's Hospital of Xinjiang Uygur Autonomous Region, Urumqi, Xinjiang Uygur Autonomous Region, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Wang
authors.affiliation: The Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou, Zhejiang Province, China.
authors.forename: Shigui
authors.initials: S
authors.lastname: Yan
authors.affiliation: People's Hospital of Xinjiang Uygur Autonomous Region, Urumqi, Xinjiang Uygur Autonomous Region, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Yuan
keywords: NSFC grants; hydatid disease; nerve regeneration; neural regeneration; neurons; radiation damage; sciatic nerve; succinate dehydrogenase; three-dimensional conformal intensity-modulated radiation therapy