title: Acoustic force spectroscopy.
abstract: Force spectroscopy has become an indispensable tool to unravel the structural and mechanochemical properties of biomolecules. Here we extend the force spectroscopy toolbox with an acoustic manipulation device that can exert forces from subpiconewtons to hundreds of piconewtons on thousands of biomolecules in parallel, with submillisecond response time and inherent stability. This method can be readily integrated in lab-on-a-chip devices, allowing for cost-effective and massively parallel applications.
authors.affiliation: 1] Department of Physics and Astronomy, VU University Amsterdam, Amsterdam, the Netherlands. [2] LaserLaB Amsterdam, VU University Amsterdam, Amsterdam, the Netherlands.
authors.forename: Gerrit
authors.initials: G
authors.lastname: Sitters
authors.affiliation: 1] Department of Physics and Astronomy, VU University Amsterdam, Amsterdam, the Netherlands. [2] LaserLaB Amsterdam, VU University Amsterdam, Amsterdam, the Netherlands.
authors.forename: Douwe
authors.initials: D
authors.lastname: Kamsma
authors.affiliation: Division for Biomedical Physics, Innsbruck Medical University, Innsbruck, Austria.
authors.forename: Gregor
authors.identifier: 0000000239488045
authors.initials: G
authors.lastname: Thalhammer
authors.affiliation: Division for Biomedical Physics, Innsbruck Medical University, Innsbruck, Austria.
authors.forename: Monika
authors.initials: M
authors.lastname: Ritsch-Marte
authors.affiliation: 1] Department of Physics and Astronomy, VU University Amsterdam, Amsterdam, the Netherlands. [2] LaserLaB Amsterdam, VU University Amsterdam, Amsterdam, the Netherlands.
authors.forename: Erwin J G
authors.initials: EJ
authors.lastname: Peterman
authors.affiliation: 1] Department of Physics and Astronomy, VU University Amsterdam, Amsterdam, the Netherlands. [2] LaserLaB Amsterdam, VU University Amsterdam, Amsterdam, the Netherlands.
authors.forename: Gijs J L
authors.initials: GJ
authors.lastname: Wuite
mesh_terms: D000162:Acoustics / Q000295:instrumentation*; D000937:Antigen-Antibody Reactions; D055592:Biophysical Phenomena; D004247:DNA / Q000737:chemistry; D004268:DNA-Binding Proteins / Q000737:chemistry; D004076:Digoxigenin / Q000276:immunology; D004867:Equipment Design; D056656:Lab-On-A-Chip Devices; D044085:Microfluidics / Q000295:instrumentation; D008863:Microspheres; D008967:Molecular Biology; D011938:Rec A Recombinases / Q000737:chemistry; D013057:Spectrum Analysis / Q000295:instrumentation / Q000379:methods*
chemical_list: D004268:DNA-Binding Proteins; D004247:DNA; D011938:Rec A Recombinases; D004076:Digoxigenin