title: Analysis of the biological function of ELDF15 using an antisense recombinant expression vector.
abstract: ELDF15, homologous with AT2 receptor-interaction protein 1 (ATIP1), may play an important role in cell differentiation, proliferation, and carcinogenesis. We aimed to understand the biological function of ELDF15 via construction and transfection of a recombinant expression vector containing antisense ELDF15. Recombinant expression vectors were successfully constructed and transfected into K562 cells. A stable transfectant, known as pXJ41-asELDF15, stably produced antisense ELDF15. Compared with K562 and K562-zeo cells, K562- pXJ41-asELDF15 cells showed inhibition of cell proliferation. RT-PCR analysis showed that the expression and protein level of ELDF15 decreased significantly in K562 cells transfected with pXJ41-asELDF15. Expression of hemoglobin increased in K562 cells transfected with pXJ41-asELDF15 by benzidine staining. increases NBT reduction activity in K562 cells transfected with pXJ41-asELDF15.Colony forming efficiency in two-layer soft agar was clearly inhibited as assessed by electron microscopy. These results suggest that ELDF15 plays a potential role in cell differentiation, proliferation and carcinogenesis.
authors.affiliation: Chinese Center for Disease Control and Prevention, Beijing, China E-mail : longwang888666@aliyun.com.
authors.forename: Yan
authors.initials: Y
authors.lastname: Liu
authors.forename: Long
authors.initials: L
authors.lastname: Wang
authors.forename: Zi-Jun
authors.initials: ZJ
authors.lastname: Wang
mesh_terms: D017209:Apoptosis; D015153:Blotting, Western; D002453:Cell Cycle; D002454:Cell Differentiation; D049109:Cell Proliferation; D005822:Genetic Vectors / Q000494:pharmacology*; D006801:Humans; D007124:Immunoenzyme Techniques; D007938:Leukemia / Q000235:genetics* / Q000473:pathology* / Q000628:therapy; D009857:Oncogenes / Q000235:genetics*; D016372:RNA, Antisense / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014407:Tumor Cells, Cultured; D025521:Tumor Suppressor Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
chemical_list: C474762:MTUS1 protein, human; D016372:RNA, Antisense; D012333:RNA, Messenger; D025521:Tumor Suppressor Proteins