title: Moving forward with reactive oxygen species involvement in antimicrobial lethality.
abstract: Support for the contribution of reactive oxygen species (ROS) to antimicrobial lethality has been refined and strengthened. Killing by diverse antimicrobials is enhanced by defects in genes that protect against ROS, inhibited by compounds that block hydroxyl radical accumulation, and is associated with surges in intracellular ROS. Moreover, support has emerged for a genetic pathway that controls the level of ROS. Since some antimicrobials kill in the absence of ROS, ROS must add to, rather than replace, known killing mechanisms. New work has addressed many of the questions concerning the specificity of dyes used to detect intracellular ROS and the specificity of perturbations that influence ROS surges. However, complexities associated with killing under anaerobic conditions remain to be resolved. Distinctions among primary lesion formation, resistance, direct lesion-mediated killing and a self-destructive stress response are discussed to facilitate efforts to potentiate ROS-mediated bacterial killing and improve antimicrobial efficacy.
authors.affiliation: Public Health Research Institute, New Jersey Medical School, Rutgers Biomedical and Health Sciences, 225 Warren Street, Newark, NJ 07103, USA Department of Microbiology and Molecular Genetics, New Jersey Medical School, Rutgers Biomedical and Health Sciences, 225 Warren Street, Newark, NJ 07103, USA State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Public Health, Xiamen University, South Xiang-An Road, Xiang-An District, Xiamen, Fujian Province 361102, China.
authors.forename: Xilin
authors.initials: X
authors.lastname: Zhao
authors.affiliation: Public Health Research Institute, New Jersey Medical School, Rutgers Biomedical and Health Sciences, 225 Warren Street, Newark, NJ 07103, USA.
authors.forename: Yuzhi
authors.initials: Y
authors.lastname: Hong
authors.affiliation: Public Health Research Institute, New Jersey Medical School, Rutgers Biomedical and Health Sciences, 225 Warren Street, Newark, NJ 07103, USA Department of Microbiology and Molecular Genetics, New Jersey Medical School, Rutgers Biomedical and Health Sciences, 225 Warren Street, Newark, NJ 07103, USA drlicaka@njms.rutgers.edu.
authors.forename: Karl
authors.initials: K
authors.lastname: Drlica
mesh_terms: D001419:Bacteria / Q000187:drug effects*; D050296:Microbial Viability / Q000187:drug effects; D017382:Reactive Oxygen Species / Q000633:toxicity*
chemical_list: D017382:Reactive Oxygen Species
keywords: ROS; antibiotics; killing; post-damage cellular response