title: The pathology of bone tissue during peri-implantitis.
abstract: Dental implants are one of the most frequently used treatment options for tooth replacement. Approximately 30% of patients with dental implants develop peri-implantitis, which is an oral inflammatory disease that leads to loss of the supporting tissues, predominately the bone. For the development of future therapeutic strategies, it is essential to understand the molecular pathophysiology of human dental peri-implant infections. Here, we describe the gene and protein expression patterns of peri-implantitis bone tissue compared with healthy peri-implant bone tissue. Furthermore, cells from the osteoblastic lineage derived from peri-implantitis samples were immortalized and characterized. We applied microarray, quantitative reverse transcription polymerase chain reaction, fluorescence-activated cell sorting, and Western blot analyses. The levels of typical bone matrix molecules, including SPP1, BGLAP, and COL9A1, in patients with peri-implantitis were reduced, while the inflammation marker interleukin 8 (IL8) was highly expressed. RUNX2, one of the transcription factors of mature osteoblasts, was also decreased in peri-implantitis. Finally, the human telomerase reverse transcriptase immortalized cell line from peri-implantitis exhibited a more fibro-osteoblastic character than did the healthy control.
authors.affiliation: Tissue Regeneration and Oral Biology Work Group, Department of Prosthodontics, Georg August University, Goettingen, Germany.
authors.forename: B
authors.initials: B
authors.lastname: Schminke
authors.affiliation: Tissue Regeneration and Oral Biology Work Group, Department of Prosthodontics, Georg August University, Goettingen, Germany.
authors.forename: F
authors.initials: F
authors.lastname: Vom Orde
authors.affiliation: Department of Oral and Maxillofacial Surgery, Georg August University, Goettingen, Germany.
authors.forename: R
authors.initials: R
authors.lastname: Gruber
authors.affiliation: Department of Oral and Maxillofacial Surgery, Georg August University, Goettingen, Germany.
authors.forename: H
authors.initials: H
authors.lastname: Schliephake
authors.affiliation: Tissue Regeneration and Oral Biology Work Group, Department of Prosthodontics, Georg August University, Goettingen, Germany.
authors.forename: R
authors.initials: R
authors.lastname: Bürgers
authors.affiliation: Tissue Regeneration and Oral Biology Work Group, Department of Prosthodontics, Georg August University, Goettingen, Germany nmiosge@gwdg.de.
authors.forename: N
authors.initials: N
authors.lastname: Miosge
mesh_terms: D000328:Adult; D000368:Aged; D000539:Alveolar Process / Q000737:chemistry / Q000473:pathology*; D001857:Bone Matrix / Q000737:chemistry / Q000473:pathology; D018929:Cell Culture Techniques; D002461:Cell Line, Transformed; D019070:Cell Lineage; D002469:Cell Separation; D002472:Cell Transformation, Viral; D024081:Collagen Type IX / Q000032:analysis; D050659:Core Binding Factor Alpha 1 Subunit / Q000032:analysis; D005260:Female; D005346:Fibroblast Growth Factors / Q000032:analysis; D005347:Fibroblasts / Q000737:chemistry / Q000473:pathology; D020869:Gene Expression Profiling; D006801:Humans; D016209:Interleukin-8 / Q000032:analysis; D008297:Male; D008875:Middle Aged; D010006:Osteoblasts / Q000737:chemistry / Q000473:pathology; D015675:Osteocalcin / Q000032:analysis; D053495:Osteopontin / Q000032:analysis; D047495:PPAR gamma / Q000032:analysis; D057873:Peri-Implantitis / Q000235:genetics / Q000473:pathology*; D019098:Telomerase / Q000032:analysis
chemical_list: C439970:COL9A1 protein, human; C508606:CXCL8 protein, human; D024081:Collagen Type IX; D050659:Core Binding Factor Alpha 1 Subunit; D016209:Interleukin-8; D047495:PPAR gamma; C493709:RUNX2 protein, human; C506895:SPP1 protein, human; C113569:fibroblast growth factor 18; D015675:Osteocalcin; D053495:Osteopontin; D005346:Fibroblast Growth Factors; C509186:TERT protein, human; D019098:Telomerase
keywords: BMP2; IL8; RUNX2; bone matrix degradation; dental implants; micro array