title: [Determination of polysorbate 80 in Reduning injection by HGPC-ELSD].
abstract: OBJECTIVE
  To establish the method for determining polysorbate 80 in Reduning injection by HPLC-ELSD, and to control the mass of polysorbate 80 in Reduning injection.
  
  
  METHODS
  It was performed by HGPC-ELSD with TOSHTSK-GEL G4000PWxl (7.8 mm x 300 mm, 10 μm). Water was used as mobile phase, the flow rate was 0.7 mL x min(-1), and the temperature was set at 30°C. The evaporated light scattering detector was adopted. The drift tube temperature was 55°C, and nitrogen was used as carrier gas, with the flow rate of 2.0 L x min(-1) and gain of 1.0.
  
  
  RESULTS
  The calibration curve showed good linearity of polysorbate 80 in the test range from 1.01 to 15.20 g x L(-1) (r2 = 0.999 3). The recovery rate was 98.10% with RSD of 2.0%.
  
  
  CONCLUSIONS
  The method is simple, rapid, accurate and reliable and suitable for the determination of polysorbate 80 in Reduning injection.
authors.forename: Juan
authors.initials: J
authors.lastname: Shen
authors.forename: Qiao
authors.initials: Q
authors.lastname: Zhang
authors.forename: Jia-Chun
authors.initials: JC
authors.lastname: Li
authors.forename: Yu-An
authors.initials: YA
authors.lastname: Bi
authors.forename: Zhen-Zhong
authors.initials: ZZ
authors.lastname: Wang
authors.forename: Wei
authors.initials: W
authors.lastname: Xiao
mesh_terms: D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D007267:Injections; D011136:Polysorbates / Q000032:analysis*; D015203:Reproducibility of Results; D013997:Time Factors
chemical_list: D004365:Drugs, Chinese Herbal; D011136:Polysorbates