title: Active ingredient-containing chitosan/polycaprolactone nonwoven mats: characterizations and their functional assays.
abstract: This study demonstrates a facile method developed to generate a chitosan/polycaprolactone (CS/PCL) nonwoven mat. All nonwoven mats are composed of microfibers with an average diameter of 2.51±0.69 μm. The X-ray photoelectron spectroscopy data indicate that positively charged nitrogen was generated on the surface of the mats after undergoing CS coating. By using a non-contacting electrostatic voltmeter, we determined that the nonwoven mats exhibited a positive potential and the charge density of the CS/PCL nonwoven mat was in proportion to the thickness of the CS overlayer. Moreover, platelet aggregation and anti-bacterial ability were enhanced by the CS/PCL nonwoven mat as compared to that of PCL nonwoven mat alone. The enhancements of the CS/PCL nonwoven mat on platelet aggregation are further promoted by incorporating a 1mM calcium ion in its CS overlayer. We also find that the addition of tea tree oil in the CS overlayer significantly inhibited LPS-induced nitrite formation in Raw 264.7 macrophages. In conclusion, our CS/PCL nonwoven mat possesses pharmacological effects including an increase of platelet aggregation, anti-bacterial, anti-adhesive, and anti-inflammatory activities. The performance of this CS/PCL nonwoven mat can be further promoted by incorporating active compounds to exert therapeutic effects in wound healing.
authors.affiliation: Graduate Institute of Biomedical Engineering, National Taiwan University of Science and Technology, Taipei 10607, Taiwan, ROC. mybai@mail.ntust.edu.tw
authors.forename: Meng-Yi
authors.initials: MY
authors.lastname: Bai
authors.affiliation: Department of Biomedical Engineering, National Defense Medical Center, Taipei 114, Taiwan, ROC. Electronic address: tcchou@ms5.hinet.net.
authors.forename: Tz-Chong
authors.initials: TC
authors.lastname: Chou
authors.affiliation: Graduate Institute of Biomedical Engineering, National Taiwan University of Science and Technology, Taipei 10607, Taiwan, ROC.
authors.forename: Jie-Chang
authors.initials: JC
authors.lastname: Tsai
authors.affiliation: Graduate Institute of Biomedical Engineering, National Taiwan University of Science and Technology, Taipei 10607, Taiwan, ROC.
authors.forename: Hui-Ching
authors.initials: HC
authors.lastname: Yang
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology; D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000494:pharmacology; D001792:Blood Platelets / Q000502:physiology; D002118:Calcium / Q000737:chemistry; D002448:Cell Adhesion / Q000187:drug effects; D002460:Cell Line; D048271:Chitosan / Q000737:chemistry*; D051379:Mice; D036103:Nanotechnology; D009391:Nephelometry and Turbidimetry; D010974:Platelet Aggregation / Q000187:drug effects; D011091:Polyesters / Q000737:chemistry*; D011817:Rabbits; D013211:Staphylococcus aureus / Q000187:drug effects; D055672:Static Electricity; D014945:Wound Healing / Q000187:drug effects
chemical_list: D000900:Anti-Bacterial Agents; D000893:Anti-Inflammatory Agents; D011091:Polyesters; C016240:polycaprolactone; D048271:Chitosan; D002118:Calcium