title: Poly(aspartic acid)-based degradable assemblies for highly efficient gene delivery.
abstract: Due to its good properties such as low cytotoxicity, degradability, and biocompatibility, poly(aspartic acid) (PAsp) is a good candidate for the development of new drug delivery systems. In this work, a series of new PAsp-based degradable supramolecular assemblies were prepared for effective gene therapy via the host-guest interactions between the cyclodextrin (CD)-cored PAsp-based polycations and the pendant benzene group-containing PAsp backbones. Such supramolecular assemblies exhibited good degradability, enhanced pDNA condensation ability, and low cytotoxicity. More importantly, the gene transfection efficiencies of supramolecular assemblies were much higher than those of CD-cored PAsp-based counterparts at various N/P ratios. In addition, the effective antitumor ability of assemblies was demonstrated with a suicide gene therapy system. The present study would provide a new means to produce degradable supramolecular drug delivery systems.
authors.affiliation: State Key Laboratory of Chemical Resource Engineering, College of Materials Science & Engineering, Beijing University of Chemical Technology , Beijing 100029, China.
authors.forename: Jing-Jun
authors.initials: JJ
authors.lastname: Nie
authors.forename: Xue-Bo
authors.initials: XB
authors.lastname: Dou
authors.forename: Hao
authors.initials: H
authors.lastname: Hu
authors.forename: Bingran
authors.initials: B
authors.lastname: Yu
authors.forename: Da-Fu
authors.initials: DF
authors.lastname: Chen
authors.forename: Ren-Xian
authors.initials: RX
authors.lastname: Wang
authors.forename: Fu-Jian
authors.initials: FJ
authors.lastname: Xu
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry; D001554:Benzene / Q000737:chemistry; D002412:Cations; D002470:Cell Survival; D003505:Cyclodextrins / Q000737:chemistry; D004247:DNA / Q000737:chemistry; D016503:Drug Delivery Systems; D015316:Genetic Therapy / Q000379:methods; D005822:Genetic Vectors; D057809:HEK293 Cells; D056945:Hep G2 Cells; D006801:Humans; D018625:Microscopy, Atomic Force; D010316:Particle Size; D010455:Peptides / Q000737:chemistry*; D010957:Plasmids / Q000378:metabolism; D011108:Polymers / Q000737:chemistry; D014162:Transfection
chemical_list: D000970:Antineoplastic Agents; D002412:Cations; D003505:Cyclodextrins; D010455:Peptides; D011108:Polymers; C017645:polyaspartate; D004247:DNA; D001554:Benzene
keywords: DNA; degradable; self-assembly; supramolecular; vector