title: Screening for potential -α -Glucosidase inhibitors in Coptis chinensis franch extract using ultrafiltration LC-ESI-MSn.
abstract: Naturally existing -α -glucosidase inhibitors from traditional herbal medicines have attracted considerable interest to treat type 2 diabetes mellitus (T2DM). Hundreds of herbs have been reported to have the potential to inhibit -α -glucosidase. However, most common methods to examine the inhibitors of -α -glucosidase are usually time-consuming. In the current study, the screening of -α -glucosidase ligands from Coptis chinensis Franch extract was undertaken by ultrafiltration liquid chromatography coupled to electrospray ionization tandem mass spectrometry (ultrafiltration LC-ESI-MS(n)). Resultantly, the enzyme inhibition studies showed that Coptis chinensis Franch extract carries the strongest -α -glucosidase inhibitory activity among the five kinds of Chinese herbal extracts. Subsequently, five compounds that could bind to -α -glucosidase in the Coptis chinensis Franch extract were found using ultrafiltration liquid chromatography, and their structures were identified by ESI-MS(n) to be coptisine, epiberberine, jatrorrhizine, berberine, palmatine. Cumulatively, these results were anticipated to be encouraging for applying the Coptis chinensis Franch extracts as efficient anti-diabetic drug candidates.
authors.affiliation: Department of Chemistry and Pharmacy, Zhuhai College of Jilin University, Zhuhai, P.R. China / Changchun Center of Mass Spectrometry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, P.R. China.
authors.forename: Hui
authors.initials: H
authors.lastname: Zhou
authors.affiliation: Department of Chemistry and Pharmacy, Zhuhai College of Jilin University, Zhuhai, P.R. China.
authors.forename: Tao
authors.initials: T
authors.lastname: Jiang
authors.affiliation: Institute of Theoretical Chemistry, Jilin University, Changchun, P.R. China.
authors.forename: Zhengyang
authors.initials: Z
authors.lastname: Wang
authors.affiliation: College of Traditional Chinese Medicine, Jilin Agricultural University, Changchun, P.R. China.
authors.forename: Shen
authors.initials: S
authors.lastname: Ren
authors.affiliation: Department of Chemistry and Pharmacy, Zhuhai College of Jilin University, Zhuhai, P.R. China.
authors.forename: Xi
authors.initials: X
authors.lastname: Zhao
authors.affiliation: Department of Biomedical Sciences, Texas Tech University Health Sciences Center, School of Pharmacy, Amarillo, TX USA.
authors.forename: Wei
authors.initials: W
authors.lastname: Wu
authors.affiliation: Department of Chemistry and Pharmacy, Zhuhai College of Jilin University, Zhuhai, P.R. China.
authors.forename: Lianhai
authors.initials: L
authors.lastname: Jiang
authors.affiliation: Changchun Center of Mass Spectrometry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, P.R. China.
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Department of Chemistry and Pharmacy, Zhuhai College of Jilin University, Zhuhai, P.R. China.
authors.forename: Lirong
authors.initials: L
authors.lastname: Teng
mesh_terms: D002851:Chromatography, High Pressure Liquid; D039323:Coptis / Q000737:chemistry*; D065089:Glycoside Hydrolase Inhibitors / Q000032:analysis*; D010936:Plant Extracts / Q000032:analysis*; D021241:Spectrometry, Mass, Electrospray Ionization; D014462:Ultrafiltration
chemical_list: D065089:Glycoside Hydrolase Inhibitors; D010936:Plant Extracts