title: A substrate-based approach for the selection of oil-bearing heterotrophs from nitrogen-deficient soil for lipid production.
abstract: In this study, nine heterotrophic yeast isolates were tested for their ability to assimilate crude glycerol and consecutive conversion to triacylglycerides (TGAs). All the organisms were initially screened on crude glycerol-based selection media, and those producing lipid globules were further evaluated for lipid production. Sudan Black B staining of eight isolates showed lipid globules. These strains were further studied at different C/N ratio. The molecular identification revealed that the isolates belonged to the genera of Yarrowia and Candida. Among these isolates, SKY7 (Yarrowia lipolytica) produced up to 42.04 ± 0.11 % of lipid w/w) with a C/N ratio of 100 and fermentation time of 72 h. The other strains produced 5.82 ± 0.4 to 34.57 ± 0.44 % lipid (w/w). The GC-flame ionization detector (FID) lipid profile showed that the lipid produced by the strains had close resemblance with vegetable oil and could serve as a feedstock for biodiesel production. Biolog test of the isolates revealed a wide spectrum of carbon utilization.
authors.affiliation: Institut national de la recherche scientifique, Centre Eau, Terre & Environnement, Université du Québec, 490, rue de la Couronne, Quebec, G1K 9A9, Canada.
authors.forename: M
authors.initials: M
authors.lastname: Kuttiraja
authors.forename: Shyam
authors.initials: S
authors.lastname: Krishna
authors.forename: Ayed
authors.initials: A
authors.lastname: Dhouha
authors.forename: Rajeshwar Dayal
authors.initials: RD
authors.lastname: Tyagi
mesh_terms: D000596:Amino Acids / Q000378:metabolism; D001391:Azo Compounds; D056804:Biofuels; D002175:Candida / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D003470:Culture Media / Q000737:chemistry; D005285:Fermentation; D005990:Glycerol / Q000378:metabolism; D009005:Monosaccharides / Q000378:metabolism; D016533:Mycological Typing Techniques; D009281:Naphthalenes; D009844:Oligosaccharides / Q000378:metabolism; D010802:Phylogeny; D012337:RNA, Ribosomal, 18S / Q000235:genetics*; D012987:Soil / Q000737:chemistry; D012988:Soil Microbiology; D014280:Triglycerides / Q000096:biosynthesis*; D025062:Yarrowia / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D000596:Amino Acids; D001391:Azo Compounds; D056804:Biofuels; D003470:Culture Media; D009005:Monosaccharides; D009281:Naphthalenes; D009844:Oligosaccharides; D012337:RNA, Ribosomal, 18S; D012987:Soil; D014280:Triglycerides; C016118:Sudan Black B; D005990:Glycerol