title: [Production of extracellular proteinases (protein C activators in blood plasma) by the micromycete Aspergillus ochraceus during submerged and solid-state cultivation].
abstract: The conditions for the submerged and solid-state cultivation of the micromycete Aspergillus ochraceus VKM F-4104D, producing extracellular proteinases that activate protein C in human blood plasma, were optimized. It is shown that the protein C-activating activity of the micromycete in a solid-state culture was 1.5-3.5 times higher than in a submerged culture (as calculated per milliliter of culture medium). Among the extracellular proteins secreted by A. ochraceus VKM F-4104D during submerged and solid-state cultivation, a protein C-activating proteinase with a pI of 6.0-6.3 was identified.
authors.forename: A A
authors.initials: AA
authors.lastname: Osmolovskiĭ
authors.forename: V G
authors.initials: VG
authors.lastname: Kreĭer
authors.forename: N A
authors.initials: NA
authors.lastname: Baranova
authors.forename: A V
authors.initials: AV
authors.lastname: Kurakov
authors.forename: N S
authors.initials: NS
authors.lastname: Egorov
mesh_terms: D001230:Aspergillus / Q000201:enzymology*; D005656:Fungal Proteins / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D010447:Peptide Hydrolases / Q000737:chemistry / Q000378:metabolism*; D010949:Plasma / Q000737:chemistry*; D011486:Protein C / Q000737:chemistry*
chemical_list: D005656:Fungal Proteins; D011486:Protein C; D010447:Peptide Hydrolases