title: Housing temperature influences the pattern of heat shock protein induction in mice following mild whole body hyperthermia.
abstract: OBJECTIVE
  Researchers studying the murine response to stress generally use mice housed under standard, nationally mandated conditions as controls. Few investigators are concerned whether basic physical aspects of mouse housing could be an additional source of stress, capable of influencing the subsequent impact of an experimentally applied stressor. We have recently become aware of the potential for housing conditions to impact important physiological and immunological properties in mice.
  
  
  METHODS
  Here we sought to determine whether housing mice at standard temperature (ST; 22 °C) vs. thermoneutral temperature (TT; 30 °C) influences baseline expression of heat shock proteins (HSPs) and their typical induction following a whole body heating.
  
  
  RESULTS
  There were no significant differences in baseline expression of HSPs at ST and TT. However, in several cases, the induction of Hsp70, Hsp110 and Hsp90 in tissues of mice maintained at ST was greater than at TT following 6 h of heating (which elevated core body temperature to 39.5 °C). This loss of HSP induction was also seen when mice housed at ST were treated with propranolol, a β-adrenergic receptor antagonist, used clinically to treat hypertension and stress.
  
  
  CONCLUSIONS
  Taken together, these data show that housing temperature significantly influences the expression of HSPs in mice after whole body heating and thus should be considered when stress responses are studied in mice.
authors.affiliation: Department of Immunology, Roswell Park Cancer Institute , Buffalo, New York , USA.
authors.forename: Jason W-L
authors.initials: JW
authors.lastname: Eng
authors.forename: Chelsey B
authors.initials: CB
authors.lastname: Reed
authors.forename: Kathleen M
authors.initials: KM
authors.lastname: Kokolus
authors.forename: Elizabeth A
authors.initials: EA
authors.lastname: Repasky
mesh_terms: D000319:Adrenergic beta-Antagonists / Q000494:pharmacology; D000818:Animals; D015153:Blotting, Western / Q000379:methods; D001831:Body Temperature / Q000502:physiology*; D058639:Cold-Shock Response / Q000502:physiology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D006360:Heat-Shock Proteins / Q000378:metabolism*; D006799:Housing, Animal / Q000592:standards*; D006979:Hyperthermia, Induced; D051379:Mice; D008807:Mice, Inbred BALB C; D009638:Norepinephrine / Q000097:blood; D011433:Propranolol / Q000494:pharmacology; D013312:Stress, Physiological / Q000502:physiology
chemical_list: D000319:Adrenergic beta-Antagonists; D006360:Heat-Shock Proteins; D011433:Propranolol; D009638:Norepinephrine
keywords: Cold stress; heat shock protein; thermoneutrality; whole body hyperthermia