title: Switchavidin: reversible biotin-avidin-biotin bridges with high affinity and specificity.
abstract: Switchavidin is a chicken avidin mutant displaying reversible binding to biotin, an improved binding affinity toward conjugated biotin, and low nonspecific binding due to reduced surface charge. These properties make switchavidin an optimal tool in biosensor applications for the reversible immobilization of biotinylated proteins on biotinylated sensor surfaces. Furthermore, switchavidin opens novel possibilities for patterning, purification, and labeling.
authors.affiliation: BioMediTech, University of Tampere , Biokatu 6, FI-33520 Tampere, Finland.
authors.forename: Barbara
authors.initials: B
authors.lastname: Taskinen
authors.forename: Dominik
authors.initials: D
authors.lastname: Zauner
authors.forename: Soili I
authors.initials: SI
authors.lastname: Lehtonen
authors.forename: Masi
authors.initials: M
authors.lastname: Koskinen
authors.forename: Chloe
authors.initials: C
authors.lastname: Thomson
authors.forename: Niklas
authors.initials: N
authors.lastname: Kähkönen
authors.forename: Sampo
authors.initials: S
authors.lastname: Kukkurainen
authors.forename: Juha A E
authors.initials: JA
authors.lastname: Määttä
authors.forename: Teemu O
authors.initials: TO
authors.lastname: Ihalainen
authors.forename: Markku S
authors.initials: MS
authors.lastname: Kulomaa
authors.forename: Hermann J
authors.initials: HJ
authors.lastname: Gruber
authors.forename: Vesa P
authors.initials: VP
authors.lastname: Hytönen
mesh_terms: D016475:3T3 Cells; D000818:Animals; D001360:Avidin / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D001665:Binding Sites; D015374:Biosensing Techniques; D001710:Biotin / Q000737:chemistry*; D019921:Biotinylation; D002152:Calorimetry, Differential Scanning; D002645:Chickens; D051379:Mice; D009154:Mutation; D020349:Surface Plasmon Resonance
chemical_list: D001360:Avidin; D001710:Biotin