title: Immobilization of Acidithiobacillus ferrooxidans on sulfonated microporous poly(styrene-divinylbenzene) copolymer with granulated activated carbon and its use in bio-oxidation of ferrous iron.
abstract: The immobilization efficiencies of Acidithiobacillus ferrooxidans cells on different immobilization matrices were investigated for biooxidation of ferrous iron (Fe(2+)) to ferric iron (Fe(3+)). Six different matrices were used such as the polyurethane foam (PUF), granular activated carbon (GAC), raw poly(styrene-divinylbenzene) copolymer (rawSDVB), raw poly(styrene-divinylbenzene) copolymer with granular activated carbon (rawSDVB-GAC), sulfonated poly(styrene-divinylbenzene) copolymer (sulfSDVB) and sulfonated poly(styrene-divinylbenzene) copolymer with granular activated carbon (sulfSDVB-GAC). The sulfSDVB-GAC polymer showed the best performance for Fe(2+) biooxidation. It was used at packed-bed bioreactor and the kinetic parameters were obtained. The highest Fe(2+) biooxidation rate (R) was found to be 4.02 g/L h at the true dilution rate (Dt) of 2.47 1/h and hydraulic retention time (τ) of 0.4 h. The sulfSDVB-GAC polymer was used for the first time as immobilization material for A. ferrooxidans for Fe(2+) biooxidation.
authors.affiliation: Gebze Institute of Technology, Department of Environmental Engineering, Gebze, 41400, Kocaeli, Turkey.
authors.forename: Derya Yuksel
authors.initials: DY
authors.lastname: Koseoglu-Imer
authors.affiliation: Gebze Institute of Technology, Department of Environmental Engineering, Gebze, 41400, Kocaeli, Turkey. Electronic address: bkeskinler@gyte.edu.tr.
authors.forename: Bulent
authors.initials: B
authors.lastname: Keskinler
mesh_terms: D042763:Acidithiobacillus / Q000166:cytology* / Q000187:drug effects / Q000648:ultrastructure; D019149:Bioreactors / Q000382:microbiology; D018914:Cells, Immobilized / Q000166:cytology / Q000187:drug effects / Q000648:ultrastructure; D002606:Charcoal / Q000494:pharmacology*; D007501:Iron / Q000737:chemistry*; D007700:Kinetics; D010084:Oxidation-Reduction / Q000187:drug effects; D011137:Polystyrenes / Q000737:chemistry / Q000494:pharmacology*; D016062:Porosity; D017550:Spectroscopy, Fourier Transform Infrared; D013451:Sulfonic Acids / Q000494:pharmacology*
chemical_list: D011137:Polystyrenes; D013451:Sulfonic Acids; D002606:Charcoal; C003771:Amberlite XAD-2 resin; D007501:Iron