title: Blocking ELISA using recombinant NcSRS2 protein for diagnosing bovine neosporosis.
abstract: Neospora caninum is the etiologic agent of neosporosis, which leads to economic impacts on cattle industry. The reference method for serodiagnosis of neosporosis is the indirect fluorescent antibody test (IFAT). However, IFAT is laborious, expensive, and is not practicable in high throughput screening. In order to facilitate the serological diagnosis of neosporosis, we developed a blocking enzyme-linked immunosorbent assay (b-ELISA) based on NcSRS2 recombinant protein (rNcSRS2) and polyclonal antibodies against rNcSRS2 (b-ELISA/rNcSRS2). Compared to IFAT, b-ELISA/rNcSRS2 showed 93.7 % accuracy (98.7 % sensitivity and 88.7 % specificity), suggesting its potential as diagnostic assay to detect N. caninum antibodies in cattle sera.
authors.affiliation: Laboratório de Imunodiagnóstico, Núcleo de Biotecnologia, Centro de Desenvolvimento Tecnológico, Universidade Federal de Pelotas, CEP 96010-900, Pelotas, RS, P.O. Box 354, Brazil.
authors.forename: Francine A
authors.initials: FA
authors.lastname: Sinnott
authors.forename: Leonardo G
authors.initials: LG
authors.lastname: Monte
authors.forename: Thais F
authors.initials: TF
authors.lastname: Collares
authors.forename: Bruno M
authors.initials: BM
authors.lastname: De Matos
authors.forename: Diene B
authors.initials: DB
authors.lastname: Pacheco
authors.forename: Sibele
authors.initials: S
authors.lastname: Borsuk
authors.forename: Renato
authors.initials: R
authors.lastname: Andreotti
authors.forename: Cláudia P
authors.initials: CP
authors.lastname: Hartleben
mesh_terms: D000818:Animals; D000906:Antibodies; D019138:Antibodies, Blocking; D000953:Antigens, Protozoan / Q000276:immunology*; D000954:Antigens, Surface / Q000276:immunology*; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis* / Q000276:immunology* / Q000469:parasitology; D003048:Coccidiosis / Q000662:veterinary*; D004797:Enzyme-Linked Immunosorbent Assay; D018688:Neospora / Q000276:immunology*; D015800:Protozoan Proteins / Q000276:immunology*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D000906:Antibodies; D019138:Antibodies, Blocking; D000953:Antigens, Protozoan; D000954:Antigens, Surface; D015800:Protozoan Proteins; C115389:SRS2 protein, Neospora caninum