title: Degradation of benzotrifluoride via the dioxygenase pathway in Rhodococcus sp. 065240.
abstract: We previously isolated Rhodococcus sp. 065240, which catalyzes the defluorination of benzotrifluoride (BTF). In order to investigate the mechanism of this degradation of BTF, we performed proteomic analysis of cells grown with or without BTF. Three proteins, which resemble dioxygenase pathway enzymes responsible for isopropylbenzene degradation from Rhodococcus erythropolis BD2, were induced by BTF. Genomic PCR and DNA sequence analysis revealed that the Rhodococcus sp. 065240 carries the gene cluster, btf, which is highly homologous to the ipb gene cluster from R. erythropolis BD2. A mutant strain, which could not catalyze BTF defluorination, was isolated from 065240 strain by UV mutagenesis. The mutant strain had one mutation in the btfT gene, which encodes a response regulator of the two component system. The defluorinating ability of the mutant strain was recovered by complementation of btfT. These results suggest that the btf gene cluster is responsible for degradation of BTF.
authors.affiliation: a Department of Bioengineering , Tokyo Institute of Technology , Yokohama , Japan.
authors.forename: Kenichi
authors.initials: K
authors.lastname: Yano
authors.forename: Masaaki
authors.initials: M
authors.lastname: Wachi
authors.forename: Sakiko
authors.initials: S
authors.lastname: Tsuchida
authors.forename: Tomoya
authors.initials: T
authors.lastname: Kitazume
authors.forename: Noritaka
authors.initials: N
authors.lastname: Iwai
mesh_terms: D049109:Cell Proliferation / Q000187:drug effects; D049308:Dioxygenases / Q000378:metabolism*; D005464:Fluorobenzenes / Q000378:metabolism* / Q000494:pharmacology; D005810:Multigene Family / Q000235:genetics; D009154:Mutation; D040901:Proteomics; D012240:Rhodococcus / Q000166:cytology / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*
chemical_list: D005464:Fluorobenzenes; C044697:benzotrifluoride; D049308:Dioxygenases
keywords: Rhodococcus; benzotrifluoride; biodegradation; dioxygenase; fluorine