title: Microbial community analysis in rice paddy soils irrigated by acid mine drainage contaminated water.
abstract: Five rice paddy soils located in southwest China were selected for geochemical and microbial community analysis. These rice fields were irrigated with river water which was contaminated by Fe-S-rich acid mine drainage. Microbial communities were characterized by high-throughput sequencing, which showed 39 different phyla/groups in these samples. Among these phyla/groups, Proteobacteria was the most abundant phylum in all samples. Chloroflexi, Acidobacteria, Nitrospirae, and Bacteroidetes exhibited higher relative abundances than other phyla. A number of rare and candidate phyla were also detected. Moreover, canonical correspondence analysis suggested that pH, sulfate, and nitrate were significant factors that shaped the microbial community structure. In addition, a wide diversity of Fe- and S-related bacteria, such as GOUTA19, Shewanella, Geobacter, Desulfobacca, Thiobacillus, Desulfobacterium, and Anaeromyxobacter, might be responsible for biogeochemical Fe and S cycles in the tested rice paddy soils. Among the dominant genera, GOUTA19 and Shewanella were seldom detected in rice paddy soils.
authors.affiliation: State Key Laboratory of Environmental Geochemistry, Chinese Academy of Sciences, Guiyang, 550002, China.
authors.forename: Min
authors.initials: M
authors.lastname: Sun
authors.forename: Tangfu
authors.initials: T
authors.lastname: Xiao
authors.forename: Zengping
authors.initials: Z
authors.lastname: Ning
authors.forename: Enzong
authors.initials: E
authors.lastname: Xiao
authors.forename: Weimin
authors.initials: W
authors.lastname: Sun
mesh_terms: D000143:Acids; D001419:Bacteria / Q000145:classification* / Q000302:isolation & purification; D041963:Bacteroidetes / Q000302:isolation & purification; D018533:Biomass; D055598:Chemical Phenomena; D002681:China; D045302:Geobacter / Q000302:isolation & purification; D006863:Hydrogen-Ion Concentration; D007501:Iron / Q000032:analysis; D008906:Mining; D009566:Nitrates / Q000032:analysis; D012275:Oryza / Q000382:microbiology*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D020592:Shewanella / Q000302:isolation & purification; D012987:Soil / Q000737:chemistry; D012988:Soil Microbiology; D013431:Sulfates / Q000032:analysis; D013855:Thiobacillus / Q000302:isolation & purification; D014874:Water Pollutants, Chemical / Q000032:analysis*
chemical_list: D000143:Acids; D009566:Nitrates; D012336:RNA, Ribosomal, 16S; D012987:Soil; D013431:Sulfates; D014874:Water Pollutants, Chemical; D007501:Iron