title: Simultaneous detection of ochratoxin A and fumonisin B1 in cereal samples using an aptamer-photonic crystal encoded suspension array.
abstract: A simple, new aptamer-photonic crystal encoded suspension array was designed to simultaneously quantify and qualify ochratoxin A(OTA) and fumonisin B1(FB1) in cereal samples. The aptamers of OTA and FB1 were immobilized on the surfaces of photonic crystals by chemical bonding. When the target mycotoxins appear in a sample, the fluorescence-labeled complementary DNA of the aptamer dissociates from their double DNA hybrid and results in an obvious decrease in fluorescence intensity of the microsphere. The difference value of fluorescent intensities for each kind of silica photonic crystal microsphere (SPCM) quantitatively conveys the concentration of mycotoxin, and the structure colors or reflectance peak positions of the SPCMs confirm the kind of mycotoxin detected. The reaction conditions including the immobilization method for aptamers, hybridization, and incubation conditions have been optimized. This developed method displayed a wide linear detection range (0.01-1 ng/mL for OTA and 0.001-1 ng/mL for FB1) and a low limit of detection (0.25 pg/mL for OTA and 0.16 pg/mL for FB1). The recovery rates in the spiked cereal samples ranged from 81.80% to 116.38% for OTA and 76.58%-114.79% for FB1. The positive detection results in the naturally contaminated cereal samples were in agreement with those of classic enzyme-linked immunosorbent assay (ELISA). This simple suspension array scheme displays a great application potential for the high throughput screen assay of mycotoxins.
authors.affiliation: Department of Food Science and Nutrition, Nanjing Normal University , Nanjing 210097, China.
authors.forename: Sun
authors.initials: S
authors.lastname: Yue
authors.forename: Xu
authors.initials: X
authors.lastname: Jie
authors.forename: Li
authors.initials: L
authors.lastname: Wei
authors.forename: Cao
authors.initials: C
authors.lastname: Bin
authors.forename: Wang
authors.initials: W
authors.lastname: Dou Dou
authors.forename: Yang
authors.initials: Y
authors.lastname: Yi
authors.forename: Lin
authors.initials: L
authors.lastname: QingXia
authors.forename: Li
authors.initials: L
authors.lastname: JianLin
authors.forename: Zheng
authors.initials: Z
authors.lastname: TieSong
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry*; D002523:Edible Grain / Q000737:chemistry*; D037341:Fumonisins / Q000032:analysis*; D008863:Microspheres; D009793:Ochratoxins / Q000032:analysis*; D017785:Photons; D012822:Silicon Dioxide / Q000737:chemistry
chemical_list: D052157:Aptamers, Nucleotide; D037341:Fumonisins; D009793:Ochratoxins; C025589:ochratoxin A; C056933:fumonisin B1; D012822:Silicon Dioxide