title: Proteomic analysis of pediatric sinonasal secretions shows increased MUC5B mucin in CRS.
abstract: BACKGROUND
  Chronic rhinosinusitis (CRS) is characterized by mucous overproduction and submucosal gland hyperplasia. The global protein profile of sinonasal secretions in pediatric CRS has not been studied. We hypothesized that MUC5B, a glandular mucin, would be relatively increased in CRS secretions compared to other mucins.
  
  
  METHODS
  Secretions were collected at Children's National Health System (Children's National) from CRS patients undergoing sinus surgery and from control patients without CRS undergoing craniofacial procedures. Proteins were extracted, digested to peptides, and analyzed by mass spectometry. Fold change significance was calculated using the QSpec algorithm. Western blot analysis was performed to validate proteomic findings.
  
  
  RESULTS
  In total, 294 proteins were identified. Although both MUC5B and MUC5AC were identified in a majority of samples, the relative abundance of MUC5B was found to be significantly higher (P < 0.05). Western blot data validated these findings. Other proteins with the highest significant positive-fold change in CRS samples were BP1 fold-containing family A member 1, chitinase-3-like protein 1, plastin-2, serpin 10, and BP1 fold-containing family B member 1.
  
  
  CONCLUSIONS
  Overall, our data demonstrate an increase of MUC5B abundance in the sinus secretions of pediatric patients with CRS.
authors.affiliation: Center for Genetic Medicine Research, Children's National Medical Center, Washington, DC.
authors.forename: Amarel
authors.initials: A
authors.lastname: Saieg
authors.affiliation: 1] Center for Genetic Medicine Research, Children's National Medical Center, Washington, DC [2] Department of Integrative Systems Biology, Children's National Medical Center, Washington, DC.
authors.forename: Kristy J
authors.initials: KJ
authors.lastname: Brown
authors.affiliation: Division of Pediatric Otolaryngology-Head and Neck Surgery, Children's National Medical Center, Washington, DC.
authors.forename: Maria T
authors.initials: MT
authors.lastname: Pena
authors.affiliation: 1] Center for Genetic Medicine Research, Children's National Medical Center, Washington, DC [2] Department of Integrative Systems Biology, Children's National Medical Center, Washington, DC.
authors.forename: Mary C
authors.initials: MC
authors.lastname: Rose
authors.affiliation: 1] Center for Genetic Medicine Research, Children's National Medical Center, Washington, DC [2] Department of Integrative Systems Biology, Children's National Medical Center, Washington, DC [3] Division of Pediatric Otolaryngology-Head and Neck Surgery, Children's National Medical Center, Washington, DC.
authors.forename: Diego
authors.initials: D
authors.lastname: Preciado
mesh_terms: D000293:Adolescent; D015153:Blotting, Western; D002648:Child; D002675:Child, Preschool; D004591:Electrophoresis, Polyacrylamide Gel; D063990:Gene Ontology; D006801:Humans; D055257:Mucin-5B / Q000378:metabolism*; D009092:Mucous Membrane / Q000378:metabolism*; D010256:Paranasal Sinuses / Q000378:metabolism*; D040901:Proteomics; D012220:Rhinitis / Q000378:metabolism*; D012852:Sinusitis / Q000378:metabolism*
chemical_list: C524578:MUC5B protein, human; D055257:Mucin-5B