title: [Research progress of severed limb preservation by perfusion].
abstract: OBJECTIVE
  To summarize the research progress of severed limb preservation by perfusion and to analyze difference in effect of severed limb preservation by different perfusate.
  
  
  METHODS
  The domestic and foreign related literature about severed limb preservation by perfusion was extensively reviewed and analyzed.
  
  
  RESULTS
  Currently the main perfusate includes organ perfusate, free radical scavengers, energy mixture, blood substitutes, and whole blood. They can reduce the skeletal muscle's ischemia-reperfusion injury in different degrees.
  
  
  CONCLUSIONS
  Different perfusate can reduce the skeletal muscle's ischemia-reperfusion injury in different degrees, but the best effect of perfusate and personalized preservation method need further study.
authors.forename: Xinyuan
authors.initials: X
authors.lastname: Qin
authors.forename: Shaochun
authors.initials: S
authors.lastname: Zhang
authors.forename: Lei
authors.initials: L
authors.lastname: Gao
authors.forename: Yefeng
authors.initials: Y
authors.lastname: Yin
authors.forename: Ning
authors.initials: N
authors.lastname: Liu
authors.forename: Jiangning
authors.initials: J
authors.lastname: Wang
mesh_terms: D015925:Cryopreservation; D005121:Extremities; D006801:Humans; D018482:Muscle, Skeletal; D009926:Organ Preservation / Q000639:trends*; D010477:Perfusion; D015427:Reperfusion Injury / Q000517:prevention & control*