title: Super-SILAC: current trends and future perspectives.
abstract: Stable isotope labeling with amino acids in cell culture (SILAC) has risen as a powerful quantification technique in mass spectrometry (MS)-based proteomics in classical and modified forms. Previously, SILAC was limited to cultured cells because of the requirement of active protein synthesis; however, in recent years, it was expanded to model organisms and tissue samples. Specifically, the super-SILAC technique uses a mixture of SILAC-labeled cells as a spike-in standard for accurate quantification of unlabeled samples, thereby enabling quantification of human tissue samples. Here, we highlight the recent developments in super-SILAC and its application to the study of clinical samples, secretomes, post-translational modifications and organelle proteomes. Finally, we propose super-SILAC as a robust and accurate method that can be commercialized and applied to basic and clinical research.
authors.affiliation: Department of Human Molecular Genetics and Biochemistry, Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, 69978, Israel.
authors.forename: Anjana
authors.initials: A
authors.lastname: Shenoy
authors.forename: Tamar
authors.initials: T
authors.lastname: Geiger
mesh_terms: D000596:Amino Acids / Q000378:metabolism*; D000818:Animals; D014408:Biomarkers, Tumor / Q000378:metabolism*; D002478:Cells, Cultured; D006801:Humans; D007553:Isotope Labeling / Q000379:methods / Q000639:trends; D009369:Neoplasms / Q000175:diagnosis*; D040901:Proteomics / Q000379:methods* / Q000639:trends
chemical_list: D000596:Amino Acids; D014408:Biomarkers, Tumor
keywords: biomarkers; cancer; clinical proteomics; mass spectrometry; quantitative proteomics; spike-in standard; stable isotope labeling; super-SILAC