title: An approach for enhancing the production of human-like collagen II by enlarging the metabolic flux at pyruvate node.
abstract: As a key metabolite of glycolysis, Pyruvate affects cell growth and metabolism directly. In order to enhance the production of human-like collagen (HLC) II, pyruvate was added into fermentation broth, and response surface methodology (RSM) to optimize the addition time and dosage of pyruvate and the concentration of phosphate buffer. And the results of PB-design showed that only two variables ─ the dosage of pyruvate and the concentration of buffer ─ had significant effects on the production of human-like collagen. Quadratic polynomial models were established after analyzing the results of full factorial central composite design. The optimal dosage of pyruvate and the concentration of buffer were 8.23 mmol • L⁻¹ and 0.17 mol • L⁻¹ respectively. Under the optimal cultivation condition, Cell OD600 was increased 28.72%; the maximal production of human-like collagen could be up to 0.29 g • L⁻¹ which was 11.54% higher than that of the control group 0.26 g • L⁻¹ (the highest production of the former study).
authors.affiliation: Shaanxi R & D Center of Biomaterials and Fermentation Engineering, School of Chemical Engineering, Northwest University, Xi'an, China / Shaanxi Key Laboratory of Degradable Biomedical Materials, School of Chemical Engineering, Northwest University, Xi'an, China.
authors.forename: Ma
authors.initials: M
authors.lastname: Xiaoxuan
authors.affiliation: Shaanxi R & D Center of Biomaterials and Fermentation Engineering, School of Chemical Engineering, Northwest University, Xi'an, China / Shaanxi Key Laboratory of Degradable Biomedical Materials, School of Chemical Engineering, Northwest University, Xi'an, China.
authors.forename: Zhu
authors.initials: Z
authors.lastname: Chenhui
authors.affiliation: Shaanxi R & D Center of Biomaterials and Fermentation Engineering, School of Chemical Engineering, Northwest University, Xi'an, China / Shaanxi Key Laboratory of Degradable Biomedical Materials, School of Chemical Engineering, Northwest University, Xi'an, China.
authors.forename: Shang
authors.initials: S
authors.lastname: Zifang
authors.affiliation: Shaanxi R & D Center of Biomaterials and Fermentation Engineering, School of Chemical Engineering, Northwest University, Xi'an, China / Shaanxi Key Laboratory of Degradable Biomedical Materials, School of Chemical Engineering, Northwest University, Xi'an, China.
authors.forename: Zhang
authors.initials: Z
authors.lastname: Gaoping
authors.affiliation: Shaanxi R & D Center of Biomaterials and Fermentation Engineering, School of Chemical Engineering, Northwest University, Xi'an, China / Shaanxi Key Laboratory of Degradable Biomedical Materials, School of Chemical Engineering, Northwest University, Xi'an, China.
authors.forename: Fan
authors.initials: F
authors.lastname: Daidi
mesh_terms: D000085:Acetates / Q000378:metabolism; D018533:Biomass; D024043:Collagen Type II / Q000096:biosynthesis*; D006801:Humans; D019289:Pyruvic Acid / Q000378:metabolism*
chemical_list: D000085:Acetates; D024043:Collagen Type II; D019289:Pyruvic Acid