title: Transplantation of mature adipocyte-derived dedifferentiated fat cells promotes locomotor functional recovery by remyelination and glial scar reduction after spinal cord injury in mice.
abstract: Mature adipocyte-derived dedifferentiated fat cells (DFAT) have a potential to be useful as new cell-source for cell-based therapy for spinal cord injury (SCI), but the mechanisms remain unclear. The objective of this study was to examine whether DFAT-induced functional recovery is achieved through remyelination and/or glial scar reduction in a mice model of SCI. To accomplish this we subjected adult female mice (n=22) to SCI. On the 8th day post-injury locomotor tests were performed, and the mice were randomly divided into two groups (control and DFAT). The DFAT group received stereotaxic injection of DFAT, while the controls received DMEM medium. Functional tests were conducted at repeated intervals, until the 36th day, and immunohistochemistry or staining was performed on the spinal cord sections. DFAT transplantation significantly improved locomotor function of their hindlimbs, and promoted remyelination and glial scar reduction, when compared to the controls. There were significant and positive correlations between promotion of remyelination or/and reduction of glial scar, and recovery of locomotor function. Furthermore, transplanted DFAT expressed markers for neuron, astrocyte, and oligodendrocyte, along with neurotrophic factors, within the injured spinal cord. In conclusion, DFAT-induced functional recovery in mice after SCI is probably mediated by both cell-autonomous and cell-non-autonomous effects on remyelination of the injured spinal cord.
authors.affiliation: Laboratory of Comprehensive Veterinary Medicine, College of Bioresource Sciences, Nihon University, 1866 Kameino, Fujisawa, Kanagawa 252-0880, Japan. Electronic address: brhi10509@g.nihon-u.ac.jp.
authors.forename: Hiromi
authors.initials: H
authors.lastname: Yamada
authors.affiliation: Laboratory of Comprehensive Veterinary Medicine, College of Bioresource Sciences, Nihon University, 1866 Kameino, Fujisawa, Kanagawa 252-0880, Japan. Electronic address: dice-ito@brs.nihon-u.ac.jp.
authors.forename: Daisuke
authors.initials: D
authors.lastname: Ito
authors.affiliation: Laboratory of Cell and Tissue Biology, College of Bioresource Sciences, Nihon University, 1866 Kameino, Fujisawa, Kanagawa 252-0880, Japan. Electronic address: oki.yoshinao@nihon-u.ac.jp.
authors.forename: Yoshinao
authors.initials: Y
authors.lastname: Oki
authors.affiliation: Laboratory of Comprehensive Veterinary Medicine, College of Bioresource Sciences, Nihon University, 1866 Kameino, Fujisawa, Kanagawa 252-0880, Japan. Electronic address: kitagawa.masato@nihon-u.ac.jp.
authors.forename: Masato
authors.initials: M
authors.lastname: Kitagawa
authors.affiliation: Division of Cell Regeneration and Transplantation, Department of Functional Morphology, Nihon University School of Medicine, 30-1, Ohyaguchi-Kamimachi, Itabashi-ku, Tokyo 173-8610, Japan. Electronic address: matsumoto.taro@nihon-u.ac.jp.
authors.forename: Taro
authors.initials: T
authors.lastname: Matsumoto
authors.affiliation: Laboratory of Veterinary Internal Medicine, College of Bioresource Sciences, Nihon University, 1866 Kameino, Fujisawa, Kanagawa 252-0880, Japan. Electronic address: watari@brs.nihon-u.ac.jp.
authors.forename: Tosihiro
authors.initials: T
authors.lastname: Watari
authors.affiliation: Laboratory of Cell and Tissue Biology, College of Bioresource Sciences, Nihon University, 1866 Kameino, Fujisawa, Kanagawa 252-0880, Japan. Electronic address: kkano@brs.nihon-u.ac.jp.
authors.forename: Koichiro
authors.initials: K
authors.lastname: Kano
mesh_terms: D017667:Adipocytes / Q000166:cytology / Q000637:transplantation*; D000818:Animals; D054337:Cell Dedifferentiation; D002454:Cell Differentiation; D002921:Cicatrix / Q000503:physiopathology / Q000628:therapy; D005260:Female; D008124:Locomotion; D051379:Mice; D008810:Mice, Inbred C57BL; D009186:Myelin Sheath / Q000473:pathology* / Q000502:physiology; D009414:Nerve Growth Factors / Q000032:analysis; D055495:Neurogenesis; D009474:Neurons / Q000166:cytology; D020127:Recovery of Function; D013116:Spinal Cord / Q000166:cytology / Q000502:physiology / Q000503:physiopathology*; D013119:Spinal Cord Injuries / Q000503:physiopathology / Q000628:therapy*; D058630:Spinal Cord Regeneration
chemical_list: D009414:Nerve Growth Factors
keywords: Adipocyte-derived dedifferentiated fat cell; Cell transplantation; Remyelination; Scar reduction; Spinal cord injury