title: Integrated and comparative proteomics of high-oil and high-protein soybean seeds.
abstract: We analysed the global protein expression in seeds of a high-oil soybean cultivar (Jiyu 73, JY73) by proteomics. More than 700 protein spots were detected and 363 protein spots were successfully identified. Comparison of the protein profile of JY73 with that of a high-protein cultivar (Zhonghuang 13, ZH13) revealed 40 differentially expressed proteins, including oil synthesis, redox/stress, hydrolysis and storage-related proteins. All redox/stress proteins were less or not expressed in JY73, whereas the expression of the major storage proteins, nitrogen and carbon metabolism-related proteins was higher in ZH13. Biochemical analysis of JY73 revealed that it was in a low oxidation state, with a high content of polyunsaturated fatty acids and vitamin E. Vitamin E was more active than antioxidant enzymes and protected the soybean seed in a lower oxidation state. The characteristics of high oil and high protein in soybean, we revealed, might provide a reference for soybean nutrition and soybean breeding.
authors.affiliation: Key Laboratory of Plant Molecular Physiology, Institute of Botany, the Chinese Academy of Sciences, Xiangshan, Beijing 100093, China; State Key Laboratory of Systematic and Evolutionary Botany, Institute of Botany, the Chinese Academy of Sciences, Xiangshan, Beijing 100093, China.
authors.forename: Xiu Ping
authors.initials: XP
authors.lastname: Xu
authors.affiliation: Key Laboratory of Plant Resources, Institute of Botany, the Chinese Academy of Sciences, Xiangshan, Beijing 100093, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Liu
authors.affiliation: Key Laboratory of Plant Molecular Physiology, Institute of Botany, the Chinese Academy of Sciences, Xiangshan, Beijing 100093, China.
authors.forename: Lihong
authors.initials: L
authors.lastname: Tian
authors.affiliation: Key Laboratory of Plant Molecular Physiology, Institute of Botany, the Chinese Academy of Sciences, Xiangshan, Beijing 100093, China.
authors.forename: Xiang Bai
authors.initials: XB
authors.lastname: Dong
authors.affiliation: Key Laboratory of Plant Resources, Institute of Botany, the Chinese Academy of Sciences, Xiangshan, Beijing 100093, China.
authors.forename: Shi Hua
authors.initials: SH
authors.lastname: Shen
authors.affiliation: Key Laboratory of Plant Molecular Physiology, Institute of Botany, the Chinese Academy of Sciences, Xiangshan, Beijing 100093, China. Electronic address: lqqu@ibcas.ac.cn.
authors.forename: Le Qing
authors.initials: le Q
authors.lastname: Qu
mesh_terms: D015180:Electrophoresis, Gel, Two-Dimensional; D007529:Isoflavones / Q000032:analysis; D040901:Proteomics / Q000379:methods*; D012639:Seeds / Q000737:chemistry; D013024:Soybean Oil / Q000032:analysis*; D030262:Soybean Proteins / Q000032:analysis*; D013025:Glycine max / Q000737:chemistry* / Q000254:growth & development
chemical_list: D007529:Isoflavones; D030262:Soybean Proteins; D013024:Soybean Oil
keywords: Antioxidant; Comparative analysis; Global protein expression; Proteomics; Soybean