title: Influence of attachment strategy on the thermal stability of hybridized DNA on gold surfaces.
abstract: The thermal stabilities of double-stranded DNA hybrids immobilized on gold surfaces are shown to be significantly affected by the conformation of the hybrid. To analyze this behavior, DNA probes were immobilized using attachment strategies where the nucleotides within the strand had varying levels of interactions with the gold substrate. The abilities of these probes to form double-stranded hybrids with solution DNA targets were evaluated by surface plasmon resonance (SPR) over a temperature range 25-60 °C. The measurements were used to construct thermal stability profiles for hybrids in each conformation. We observe that DNA hybrids formed with probe strands that interact extensively with the gold surface have stability profiles that are shifted lower by 5-10 °C compared to hybrids formed with end-tethered probes that have fewer interactions with the surface. The results provide an understanding of the experimental conditions in which these weaker DNA hybrids can form and show the additional complexity of evaluating denaturation profiles generated from DNA on surfaces.
authors.affiliation: Department of Chemistry and Biochemistry, University of Wisconsin-La Crosse , La Crosse, Wisconsin 54601, United States.
authors.forename: Tyler J
authors.initials: TJ
authors.lastname: Petty
authors.forename: Caleb E
authors.initials: CE
authors.lastname: Wagner
authors.forename: Aric
authors.initials: A
authors.lastname: Opdahl
mesh_terms: D015342:DNA Probes / Q000737:chemistry*; D004355:Drug Stability; D006046:Gold / Q000737:chemistry*; D008958:Models, Molecular; D009690:Nucleic Acid Conformation; D009693:Nucleic Acid Hybridization; D020349:Surface Plasmon Resonance; D013499:Surface Properties; D013696:Temperature
chemical_list: D015342:DNA Probes; D006046:Gold