title: Genotyping of Pseudomonas aeruginosa reveals high diversity, stability over time and good outcome of eradication.
abstract: BACKGROUND
  Genotyping of Pseudomonas aeruginosa (P.a) is used for surveillance at our CF clinic.
  
  
  METHODS
  P.a from 1999 to 2012 were analysed, using pulsed-field gel electrophoresis (PFGE) and multiple-locus variable number of tandem repeats analysis (MLVA).
  
  
  RESULTS
  Among 232 isolates from 104 patients, we identified 78 unique strains, of which 56 were isolated from individual patients. The B-clone was isolated from 13 patients and the camp transmission clone J-strains from 8 patients at the start of the study. There was no indication of transmission within the clinic. PFGE and MLVA clone identification was in 91% agreement. For patients who provided more than 2 P.a isolates, similar strains were identified over time for 45/49 chronically- and for 6/16 intermittently-colonized patients despite, periods of no detectable P.a after eradication therapy.
  
  
  CONCLUSIONS
  Analyses revealed high genotypic diversity, acceptable outcome of eradication therapy and no indication of cross-infection at the CF centre.
authors.affiliation: Clinical Microbiology, Sahlgrenska University Hospital, P.O.B. 7193, SE 402 34 Gothenburg, Sweden; Institute of Biomedicine, Sahlgrenska Academy, University of Gothenburg, SE 405 30 Gothenburg, Sweden. Electronic address: Ewa.Johansson@microbio.gu.se.
authors.forename: E
authors.initials: E
authors.lastname: Johansson
authors.affiliation: Clinical Microbiology, Sahlgrenska University Hospital, P.O.B. 7193, SE 402 34 Gothenburg, Sweden; Institute of Biomedicine, Sahlgrenska Academy, University of Gothenburg, SE 405 30 Gothenburg, Sweden. Electronic address: Christina.Welinder-Olsson@vgregion.se.
authors.forename: C
authors.initials: C
authors.lastname: Welinder-Olsson
authors.affiliation: Gothenburg CF Centre, Sweden; Respiratory Medicine, Bruna Stråket 11b, Sahlgrenska University Hospital, SE 413 45 Gothenburg, Sweden; Institute of Medicine, Sahlgrenska Academy, University of Gothenburg, SE 405 30 Gothenburg, Sweden. Electronic address: Marita.Gilljam@lungall.gu.se.
authors.forename: M
authors.initials: M
authors.lastname: Gilljam
authors.affiliation: Univ Paris-Sud, CNRS, Institut de Génétique et Microbiologie, UMR 8621, Orsay, France. Electronic address: Christine.Pourcel@u-psud.fr.
authors.forename: C
authors.initials: C
authors.lastname: Pourcel
authors.affiliation: Gothenburg CF Centre, Sweden; Queen Silvia Children's Hospital, SE 416 85 Gothenburg, Sweden; Institute of Clinical Science, Sahlgrenska Academy, University of Gothenburg, SE 405 30 Gothenburg, Sweden. Electronic address: Anders.Lindblad@vgregion.se.
authors.forename: A
authors.initials: A
authors.lastname: Lindblad
mesh_terms: D000293:Adolescent; D000328:Adult; D015373:Bacterial Typing Techniques; D003550:Cystic Fibrosis / Q000150:complications* / Q000382:microbiology; D004269:DNA, Bacterial / Q000235:genetics*; D016521:Electrophoresis, Gel, Pulsed-Field; D005260:Female; D005838:Genotype; D006801:Humans; D015994:Incidence; D008297:Male; D008875:Middle Aged; D058885:Multilocus Sequence Typing; D011552:Pseudomonas Infections / Q000150:complications* / Q000453:epidemiology / Q000382:microbiology; D011550:Pseudomonas aeruginosa / Q000235:genetics* / Q000302:isolation & purification; D012189:Retrospective Studies; D013548:Sweden / Q000453:epidemiology; D055815:Young Adult
chemical_list: D004269:DNA, Bacterial
keywords: Epidemiological surveillance; Genotyping; MLVA; PFGE; Pseudomonas aeruginosa