title: Respiratory neuron characterization reveals intrinsic bursting properties in isolated adult turtle brainstems (Trachemys scripta).
abstract: It is not known whether respiratory neurons with intrinsic bursting properties exist within ectothermic vertebrate respiratory control systems. Thus, isolated adult turtle brainstems spontaneously producing respiratory motor output were used to identify and classify respiratory neurons based on their firing pattern relative to hypoglossal (XII) nerve activity. Most respiratory neurons (183/212) had peak activity during the expiratory phase, while inspiratory, post-inspiratory, and novel pre-expiratory neurons were less common. During synaptic blockade conditions, ∼10% of respiratory neurons fired bursts of action potentials, with post-inspiratory cells (6/9) having the highest percentage of intrinsic burst properties. Most intrinsically bursting respiratory neurons were clustered at the level of the vagus (X) nerve root. Synaptic inhibition blockade caused seizure-like activity throughout the turtle brainstem, which shows that the turtle respiratory control system is not transformed into a network driven by intrinsically bursting respiratory neurons. We hypothesize that intrinsically bursting respiratory neurons are evolutionarily conserved and represent a potential rhythmogenic mechanism contributing to respiration in adult turtles.
authors.affiliation: Department of Comparative Biosciences, School of Veterinary Medicine, University of Wisconsin, Madison, WI 53706, United States. Electronic address: johnsons@svm.vetmed.wisc.edu.
authors.forename: Stephen M
authors.initials: SM
authors.lastname: Johnson
authors.affiliation: Department of Biological Sciences, California State University, East Bay, Hayward, CA 94542, United States.
authors.forename: Michael S
authors.initials: MS
authors.lastname: Hedrick
authors.affiliation: Neuroscience Training Program, University of Wisconsin, Madison, WI 53706, United States.
authors.forename: Bryan M
authors.initials: BM
authors.lastname: Krause
authors.affiliation: Department of Comparative Biosciences, School of Veterinary Medicine, University of Wisconsin, Madison, WI 53706, United States.
authors.forename: Jacob P
authors.initials: JP
authors.lastname: Nilles
authors.affiliation: Department of Comparative Biosciences, School of Veterinary Medicine, University of Wisconsin, Madison, WI 53706, United States.
authors.forename: Mark A
authors.initials: MA
authors.lastname: Chapman
mesh_terms: D000818:Animals; D001933:Brain Stem / Q000502:physiology*; D004594:Electrophysiology; D009474:Neurons / Q000502:physiology*; D012143:Respiratory Physiological Phenomena; D014426:Turtles
keywords: Chelonian; Pacemaker; Reptile; Rhythm generation