title: Microwave-mediated enzymatic modifications of DNA.
abstract: Here we report microwave-induced specific cleavage, ligation, dephosphorylation, and phosphorylation of nucleic acids catalyzed by restriction endonucleases, T4 DNA ligase, T4 polynucleotide kinase, and calf intestinal alkaline phosphatase. The microwave-mediated method has dramatically reduced the reaction time to 20 to 50s. In control experiments, the same reactions failed to give the desired reaction products when carried out in the same time periods but without microwave irradiation. Because the microwave method is rapid, it could be a useful alternative to the time-consuming conventional procedure for enzymatic modification of DNA.
authors.affiliation: CSIR-Institute of Genomics and Integrative Biology, Delhi 110007, India.
authors.forename: Rakha Hari
authors.initials: RH
authors.lastname: Das
authors.affiliation: CSIR-Institute of Genomics and Integrative Biology, Delhi 110007, India.
authors.forename: Rajesh
authors.initials: R
authors.lastname: Ahirwar
authors.affiliation: CSIR-Institute of Genomics and Integrative Biology, Delhi 110007, India.
authors.forename: Saroj
authors.initials: S
authors.lastname: Kumar
authors.affiliation: CSIR-Institute of Genomics and Integrative Biology, Delhi 110007, India. Electronic address: pnahar@igib.res.in.
authors.forename: Pradip
authors.initials: P
authors.lastname: Nahar
mesh_terms: D000818:Animals; D055162:Biocatalysis; D002417:Cattle; D004247:DNA / Q000378:metabolism*; D004798:Enzymes / Q000378:metabolism*; D007700:Kinetics; D008872:Microwaves; D010766:Phosphorylation
chemical_list: D004798:Enzymes; D004247:DNA
keywords: DNA; Dephosphorylation; Digestion; Kination; Ligation; Microwave