title: Fabricating polystyrene fiber-dehydrogenase assemble as a functional biocatalyst.
abstract: Immobilization of the enzymes on nano-structured materials is a promising approach to enhance enzyme stabilization, activation and reusability. This study aimed to develop polystyrene fiber-enzyme assembles to catalyze model formaldehyde to methanol dehydrogenation reaction, which is an essential step for bioconversion of CO2 to a renewable bioenergy. We fabricated and modified electrospun polystyrene fibers, which showed high capability to immobilize dehydrogenase for the fiber-enzyme assembles. Results from evaluation of biochemical activities of the fiber-enzyme assemble showed that nitriation with the nitric/sulfuric acid ratio (v/v, 10:1) and silanization treatment delivered desirable enzyme activity and long-term storage stability, showing great promising toward future large-scale applications.
authors.affiliation: School of Chemical Engineering, The University of Adelaide, 5005 SA, Australia.
authors.forename: Hongjie
authors.initials: H
authors.lastname: An
authors.affiliation: School of Chemical Engineering, The University of Adelaide, 5005 SA, Australia. Electronic address: Bo.Jin@adelaide.edu.au.
authors.forename: Bo
authors.initials: B
authors.lastname: Jin
authors.affiliation: School of Chemical Engineering, The University of Adelaide, 5005 SA, Australia.
authors.forename: Sheng
authors.initials: S
authors.lastname: Dai
mesh_terms: D000426:Alcohol Dehydrogenase / Q000378:metabolism*; D055162:Biocatalysis; D004355:Drug Stability; D004800:Enzymes, Immobilized; D005557:Formaldehyde / Q000378:metabolism; D000432:Methanol / Q000138:chemical synthesis; D057139:Nanofibers; D017942:Nitric Acid / Q000494:pharmacology; D010084:Oxidation-Reduction; D011137:Polystyrenes; D011437:Propylamines; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism; D012821:Silanes; D013052:Spectrometry, X-Ray Emission; D017550:Spectroscopy, Fourier Transform Infrared; D013059:Spectrum Analysis, Raman; D013464:Sulfuric Acids / Q000494:pharmacology; D013499:Surface Properties
chemical_list: D004800:Enzymes, Immobilized; D011137:Polystyrenes; D011437:Propylamines; D029701:Saccharomyces cerevisiae Proteins; D012821:Silanes; D013464:Sulfuric Acids; C088294:3-aminopropyltrimethoxysilane; D005557:Formaldehyde; D017942:Nitric Acid; D000426:Alcohol Dehydrogenase; C033158:sulfuric acid; D000432:Methanol
keywords: Biocatalysis; Electrospun polystyrene fiber; Enzyme immobilization; Nitration