title: Bio-potency of a 21 kDa Kunitz-type trypsin inhibitor from Tamarindus indica seeds on the developmental physiology of H. armigera.
abstract: A trypsin inhibitor purified from the seeds of Tamarindus indica by Sephadex G-75, DEAE-Sepharose and Trypsin-Sepharose CL-4B columns was studied for its antifeedant, larvicidal, pupicidal and growth inhibitory activities against Helicoverpa armigera larvae. Tamarindus trypsin inhibitor (TTI) exhibited inhibitory activity towards total gut proteolytic enzymes of H. armigera (~87%) and bovine trypsin (~84%). Lethal doses which caused mortality and weight reduction by 50% were 1% w/w and 0.50% w/w, respectively. IC50 of TTI against Helicoverpa midgut proteases and bovine trypsin were ~2.10 µg/ml and 1.68 µg/ml respectively. In larval feeding studies the 21 kDa Kunitz-type protein was found to retard growth and development, prolonged the larval-pupal development durations along with adversely affecting the fertility and fecundity of H. armigera. In artificial diet at 0.5% w/w TTI, the efficiency of conversion of ingested food as well as of digested food, relative growth rate, growth index declined whereas approximate digestibility, metabolic cost, relative consumption rate, consumption index and total developmental period enhanced for H. armigera larvae. These results suggest that TTI has toxic and adverse effect on the developmental physiology of H. armigera and could be useful in controlling the pest H. armigera.
authors.affiliation: Department of Biochemistry [DST-FIST UGC-SAP Supported], Dr. Ram Manohar Lohia Avadh University, Faizabad, Uttar Pradesh 224001, India.
authors.forename: Prabhash K
authors.initials: PK
authors.lastname: Pandey
authors.affiliation: Department of Biochemistry [DST-FIST UGC-SAP Supported], Dr. Ram Manohar Lohia Avadh University, Faizabad, Uttar Pradesh 224001, India. Electronic address: farrukhrmlau@gmail.com.
authors.forename: Farrukh
authors.initials: F
authors.lastname: Jamal
mesh_terms: D000818:Animals; D005260:Female; D005298:Fertility / Q000187:drug effects; D041981:Gastrointestinal Tract / Q000187:drug effects / Q000201:enzymology; D007306:Insecticides / Q000633:toxicity*; D007814:Larva / Q000187:drug effects; D008297:Male; D009036:Moths / Q000187:drug effects* / Q000254:growth & development / Q000502:physiology; D010447:Peptide Hydrolases / Q000378:metabolism; D012639:Seeds; D029861:Tamarindus; D014361:Trypsin Inhibitors / Q000633:toxicity*
chemical_list: D007306:Insecticides; D014361:Trypsin Inhibitors; D010447:Peptide Hydrolases
keywords: Developmental physiology; Fecundity; H. armigera; Larvicidal; Toxicity; Trypsin inhibitor