title: Gold nanoparticles functionalization notably decreases radiosensitization through hydroxyl radical production under ionizing radiation.
abstract: The purpose of this work was to study the influence of gold nanoparticles (GNP) coating on hydroxyl radical (HO) production under ionizing radiation. Though radiosensitizing mechanisms are still unknown, radical oxygen species are likely to be involved, especially HO. We synthesized six different types of GNP, choosing relevant ligands such as polyethylene glycol or human serum albumin. A great attention was paid to characterize these GNP in terms of size, charge and number of atoms in the coating. Our results show that functionalization dramatically decreases HO production, which is correlated to reduced plasmidic DNA damages. These findings are of high importance as GNP translation from fundamental research to applied medicine requires their functionalization to increase blood circulation time and specific cancerous cells addressing. We suggest that to keep GNP efficient for radiotherapy, a wispy coating is required.
authors.affiliation: Laboratoire de Chimie Physique, CNRS UMR 8000, Université Paris-Sud, 91405 Orsay Cedex, France.
authors.forename: Manon
authors.initials: M
authors.lastname: Gilles
authors.affiliation: Laboratoire de Chimie Physique, CNRS UMR 8000, Université Paris-Sud, 91405 Orsay Cedex, France.
authors.forename: Emilie
authors.initials: E
authors.lastname: Brun
authors.affiliation: Laboratoire de Chimie Physique, CNRS UMR 8000, Université Paris-Sud, 91405 Orsay Cedex, France. Electronic address: cecile.sicard@u-psud.fr.
authors.forename: Cécile
authors.initials: C
authors.lastname: Sicard-Roselli
mesh_terms: D004249:DNA Damage / Q000528:radiation effects*; D006046:Gold / Q000737:chemistry*; D017665:Hydroxyl Radical / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry*; D011839:Radiation, Ionizing
chemical_list: D017665:Hydroxyl Radical; D006046:Gold
keywords: DNA damage; Gold nanoparticles; Hydroxyl radical; Nanoparticle coating; Radiosensitization