title: Differential effects of triacylglycerol positional isomers containing n-3 series highly unsaturated fatty acids on lipid metabolism in C57BL/6J mice.
abstract: The present study investigated the effects of binding position of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) to triacylglycerol (TAG) on lipid metabolism in C57BL/6J mice. Mice were treated with pure TAG positional isomers, including 1,2(2,3)-dipalmitoyl-3(1)-eicosapentaenoyl glycerol, 1,3-dipalmitoyl-2-eicosapentaenoyl glycerol, 1,2(2,3)-dipalmitoyl-3(1)-docosahexaenoyl glycerol, and 1,3-dipalmitoyl-2-docosahexaenoyl glycerol. Compared to DHA bound to the α-position of TAG, DHA bound to the β-position more effectively inhibited fatty acid synthetic enzymes and cholesterol-metabolism enzymes and thus reduced TAG and cholesterol concentrations in the serum and liver. EPA bound to the α-position of TAG, but not EPA bound to the β-position of TAG, significantly decreased hepatic cholesterol concentrations. Additionally, EPA bound to the α-position of TAG increased the ratio of PGI2 to TXA2 to a higher degree than EPA bound to the β-position. These results suggested that the binding position of EPA and DHA to TAG affected TAG and cholesterol metabolism as well as eicosanoid production in C57BL/6J mice.
authors.affiliation: Tsukishima Foods Industry Co. Ltd., 3-17-9, Higashi Kasai, Edogawa-ku, Tokyo 134-8520, Japan; Department of Food Science and Technology, Tokyo University of Marine Science and Technology, 4-5-7 Konan, Minato-ku, Tokyo 108-8477, Japan.
authors.forename: Kazuaki
authors.initials: K
authors.lastname: Yoshinaga
authors.affiliation: Department of Food Science and Technology, Tokyo University of Marine Science and Technology, 4-5-7 Konan, Minato-ku, Tokyo 108-8477, Japan.
authors.forename: Keiichi
authors.initials: K
authors.lastname: Sasaki
authors.affiliation: Department of Health Science, University of Kochi, 2751-1 Ike, Kochi-shi, Kochi 781-0111, Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Watanabe
authors.affiliation: Department of Applied Biochemistry and Food Science, Saga University, 1 Honjo-machi, Saga-shi, Saga 840-8502, Japan.
authors.forename: Koji
authors.initials: K
authors.lastname: Nagao
authors.affiliation: Department of Applied Biochemistry and Food Science, Saga University, 1 Honjo-machi, Saga-shi, Saga 840-8502, Japan.
authors.forename: Nao
authors.initials: N
authors.lastname: Inoue
authors.affiliation: Department of Applied Biochemistry and Food Science, Saga University, 1 Honjo-machi, Saga-shi, Saga 840-8502, Japan.
authors.forename: Bungo
authors.initials: B
authors.lastname: Shirouchi
authors.affiliation: Department of Health and Nutrition Science, Nishikyushu University, 4490-9 Ozaki, Kanzaki-machi, Kanzaki-shi, Saga 842-8585, Japan.
authors.forename: Teruyoshi
authors.initials: T
authors.lastname: Yanagita
authors.affiliation: Tsukishima Foods Industry Co. Ltd., 3-17-9, Higashi Kasai, Edogawa-ku, Tokyo 134-8520, Japan.
authors.forename: Toshiharu
authors.initials: T
authors.lastname: Nagai
authors.affiliation: Tsukishima Foods Industry Co. Ltd., 3-17-9, Higashi Kasai, Edogawa-ku, Tokyo 134-8520, Japan.
authors.forename: Hoyo
authors.initials: H
authors.lastname: Mizobe
authors.affiliation: Tsukishima Foods Industry Co. Ltd., 3-17-9, Higashi Kasai, Edogawa-ku, Tokyo 134-8520, Japan.
authors.forename: Koichi
authors.initials: K
authors.lastname: Kojima
authors.affiliation: Department of Food Science and Technology, Tokyo University of Marine Science and Technology, 4-5-7 Konan, Minato-ku, Tokyo 108-8477, Japan.
authors.forename: Fumiaki
authors.initials: F
authors.lastname: Beppu
authors.affiliation: Department of Food Science and Technology, Tokyo University of Marine Science and Technology, 4-5-7 Konan, Minato-ku, Tokyo 108-8477, Japan. Electronic address: ngotoh@kaiyodai.ac.jp.
authors.forename: Naohiro
authors.initials: N
authors.lastname: Gotoh
mesh_terms: D000284:Administration, Oral; D000818:Animals; D008076:Cholesterol, HDL / Q000097:blood; D008078:Cholesterol, LDL / Q000097:blood; D004281:Docosahexaenoic Acids / Q000008:administration & dosage* / Q000097:blood; D015118:Eicosapentaenoic Acid / Q000008:administration & dosage* / Q000097:blood; D007536:Isomerism; D050356:Lipid Metabolism / Q000187:drug effects*; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D019308:Palmitic Acid / Q000008:administration & dosage; D014280:Triglycerides / Q000008:administration & dosage* / Q000097:blood
chemical_list: D008076:Cholesterol, HDL; D008078:Cholesterol, LDL; D014280:Triglycerides; D004281:Docosahexaenoic Acids; D019308:Palmitic Acid; D015118:Eicosapentaenoic Acid
keywords: C57BL/6J mouse; Docosahexaenoic acid; Eicosapentaenoic acid; Lipid metabolism; Positional isomer; Triacylglycerol