title: A lack of confirmation with alternative assays questions the validity of IL-17A expression in human neutrophils using immunohistochemistry.
abstract: We identified IL-17A-positive neutrophils in Wolbachia-positive Onchocerca volvulus nodules using an antibody that has previously reported IL-17A-positive neutrophils in several inflammatory conditions. However, we could not detect IL-17A using a range of alternative assays. Our data question the IL-17A antibody specificity and the ability of human neutrophils to express IL-17A.
authors.affiliation: Department of Parasitology, Liverpool School of Tropical Medicine, Liverpool, UK.
authors.forename: Francesca
authors.initials: F
authors.lastname: Tamarozzi
authors.affiliation: Institute of Integrative Biology, University of Liverpool, Liverpool, UK.
authors.forename: Helen L
authors.initials: HL
authors.lastname: Wright
authors.affiliation: Institute of Integrative Biology, University of Liverpool, Liverpool, UK.
authors.forename: Huw B
authors.initials: HB
authors.lastname: Thomas
authors.affiliation: Institute of Integrative Biology, University of Liverpool, Liverpool, UK.
authors.forename: Steven W
authors.initials: SW
authors.lastname: Edwards
authors.affiliation: Department of Parasitology, Liverpool School of Tropical Medicine, Liverpool, UK. Electronic address: Mark.Taylor@lstmed.ac.uk.
authors.forename: Mark J
authors.initials: MJ
authors.lastname: Taylor
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000737:chemistry* / Q000276:immunology; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects / Q000276:immunology; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D020381:Interleukin-17 / Q000276:immunology*; D008297:Male; D009504:Neutrophils / Q000276:immunology* / Q000473:pathology; D017181:Onchocerca volvulus / Q000276:immunology*; D009855:Onchocerciasis / Q000188:drug therapy / Q000276:immunology* / Q000473:pathology
chemical_list: D000909:Antibodies, Helminth; C508717:IL17A protein, human; D020381:Interleukin-17
keywords: IL-17A; Interleukin-17; Neutrophil; Wolbachia