title: A disposable electrochemical sensor for simultaneous determination of norepinephrine and serotonin in rat cerebrospinal fluid based on MWNTs-ZnO/chitosan composites modified screen-printed electrode.
abstract: A new electrochemical sensor for simultaneous determination of norepinephrine (NE) and serotonin (5-HT) was fabricated. The electrochemical behavior of NE and 5-HT were investigated using CV and SWV at the MWNTs-ZnO/chitosan composites modified screen-printed electrode (MWNTs-ZnO/chitosan SPE). The results showed that the current responses of NE and 5-HT greatly enhanced due to the high catalytic activity of composites. The peak potentials of NE and 5-HT were separated at about 90mV and 280mV, respectively. The peak currents of NE and 5-HT were linearly dependent on their concentrations in the range of 0.5-30μM and 0.05-1μM, with the limit of detection of 0.2μM and 0.01μM, respectively. The modified electrode can be stored stably for at least 3 month at 4°C in a refrigerator. Furthermore, the modified electrode was successfully applied to detect the level of NE and 5-HT in rat cerebrospinal fluid (CSF) with excellent selectivity and sensitivity.
authors.affiliation: Department of Pharmacy, Wuhan University, Wuhan 430072, China.
authors.forename: Yuting
authors.initials: Y
authors.lastname: Wang
authors.affiliation: School of Pharmacy, Hubei University of Science and Technology, Xianning 437100, China.
authors.forename: Shi
authors.initials: S
authors.lastname: Wang
authors.affiliation: Department of Pharmacy, Wuhan University, Wuhan 430072, China.
authors.forename: Li
authors.initials: L
authors.lastname: Tao
authors.affiliation: School of Pharmacy, Hubei University of Science and Technology, Xianning 437100, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Min
authors.affiliation: Department of Pharmacy, Wuhan University, Wuhan 430072, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Xiang
authors.affiliation: Department of Pharmacy, Wuhan University, Wuhan 430072, China.
authors.forename: Qiman
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Department of Pharmacy, Wuhan University, Wuhan 430072, China.
authors.forename: Jianmei
authors.initials: J
authors.lastname: Xie
authors.affiliation: Department of Pharmacy, Wuhan University, Wuhan 430072, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Yue
authors.affiliation: Department of Pharmacy, Wuhan University, Wuhan 430072, China.
authors.forename: Shuangchan
authors.initials: S
authors.lastname: Wu
authors.affiliation: Department of Pharmacy, Wuhan University, Wuhan 430072, China.
authors.forename: Xiaofei
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Pharmacy, Wuhan University, Wuhan 430072, China. Electronic address: dinghong1106@whu.edu.cn.
authors.forename: Hong
authors.initials: H
authors.lastname: Ding
mesh_terms: D000818:Animals; D015374:Biosensing Techniques / Q000295:instrumentation; D048271:Chitosan / Q000737:chemistry*; D055664:Electrochemical Techniques / Q000295:instrumentation*; D004566:Electrodes; D004867:Equipment Design; D057230:Limit of Detection; D037742:Nanotubes, Carbon / Q000737:chemistry* / Q000648:ultrastructure; D009638:Norepinephrine / Q000134:cerebrospinal fluid*; D051381:Rats; D012701:Serotonin / Q000134:cerebrospinal fluid*; D015034:Zinc Oxide / Q000737:chemistry*
chemical_list: D037742:Nanotubes, Carbon; D012701:Serotonin; D048271:Chitosan; D015034:Zinc Oxide; D009638:Norepinephrine
keywords: Modified screen printed electrode; Norepinephrine; Rat cerebrospinal fluid samples; Serotonin; Simultaneous determination