title: Changes in expression of Slit1 and its receptor Robo2 in trigeminal ganglion and inferior alveolar nerve following inferior alveolar nerve axotomy in adult rats: a pilot study.
abstract: The objective of this study was to analyze changes in expression pattern of Slit1 and Robo2, and to clarify the relationship between these changes and functional recovery of the axotomized inferior alveolar nerve (IAN) without repair using a rat IAN axotomy model. Slit1 and Robo2 were weakly expressed in samples taken from trigeminal ganglion (TG) and IAN of sham surgery rats. In axotomized rats, expression levels increased significantly from day 2 to day 28 post-axotomy, with peaks on days 14 (Slit1) and 7 (Robo2) after axotomy (relative to sham: Slit1 in TG P<0.0005, Slit1 in IAN P = 0.003, Robo2 in TG P<0.0005, and Robo2 in IAN P<0.0005). Over-expressed Slit1 and Robo2 in both the TG and IANs of axotomized rats did not return to sham levels during the 28-day observation period of this study. The regeneration and functional recovery of axotomized IAN was evaluated by jaw opening reflex (JOR) recorded before and after axotomy. JOR occurrence (0% on day 7, 35% on day 14, and 85% on day 28) increased gradually, and the relative threshold of electrical stimulation eliciting JOR decreased gradually (1000.0 ± 0.0% on day 7, 854.3 ± 132.5% on day 14, and 302.6 ± 92.3% on day 28). On day 28 after axotomy, JOR occurrence and the relative JOR threshold had almost returned to those of sham rats. These findings suggest that Slit1 and Robo2 are involved in the regeneration and functional recovery of the axotomized IAN.
authors.affiliation: Department of Plastic, Reconstructive and Aesthetic Surgery, Faculty of Medicine, Namik Kemal University, Tekirdag, Turkey. Electronic address: mdceber@yahoo.com.
authors.forename: M
authors.initials: M
authors.lastname: Ceber
authors.affiliation: Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Bezmialem Vakif University, Istanbul, Turkey.
authors.forename: A
authors.initials: A
authors.lastname: Mihmanli
authors.affiliation: Department of Medical Biology, Faculty of Medicine, Bezmialem Vakif University, Istanbul, Turkey.
authors.forename: U
authors.initials: U
authors.lastname: Kilic
authors.affiliation: Department of Physiology, Faculty of Medicine, Namik Kemal University, Tekirdag, Turkey.
authors.forename: U
authors.initials: U
authors.lastname: Sener
authors.affiliation: Department of Anaesthesiology, Faculty of Medicine, Namik Kemal University, Tekirdag, Turkey.
authors.forename: A
authors.initials: A
authors.lastname: Yuksek
authors.affiliation: Department of Neurosurgery, Faculty of Medicine, Namik Kemal University, Tekirdag, Turkey.
authors.forename: M A
authors.initials: MA
authors.lastname: Durak
authors.affiliation: Department of Biochemistry, Faculty of Medicine, Namik Kemal University, Tekirdag, Turkey.
authors.forename: F
authors.initials: F
authors.lastname: Tulubas
mesh_terms: D000818:Animals; D019771:Axotomy; D015153:Blotting, Western; D004586:Electrophoresis; D004594:Electrophysiology; D008340:Mandibular Nerve / Q000378:metabolism* / Q000601:surgery*; D009416:Nerve Regeneration / Q000502:physiology*; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D010865:Pilot Projects; D051381:Rats; D017207:Rats, Sprague-Dawley; D011971:Receptors, Immunologic / Q000378:metabolism*; D013997:Time Factors; D012668:Trigeminal Ganglion / Q000378:metabolism*; D000097905:Roundabout Proteins
chemical_list: D009419:Nerve Tissue Proteins; D011971:Receptors, Immunologic; C516057:Robo2 protein, rat; D000097905:Roundabout Proteins; C492288:Slit1 protein, rat
keywords: Robo2; Slit1; axotomy; inferior alveolar nerve; jaw opening reflex; nerve regeneration