title: Dynamics of bacterial microbiota during lignocellulosic waste composting: Studies upon its structure, functionality and biodiversity.
abstract: An intensive isolation program carried out in three replicated composting piles allowed the identification of the resident and transient components of the composting microbiome. More than 4000 bacterial strains were isolated, enzymatically characterized and identified by partial sequencing of their 16S rRNA gene. While microorganisms isolated under mesophilic conditions were prominent throughout the process, thermophilic stages gathered the highest total counts and spore-forming bacteria prevailed at the bio-oxidative phase of composting. Enzymatic capabilities related to the degradation of polymeric materials were exhibited by most of the isolates and as a result of these activities, more soluble compounds could be made available to the entire composting microbiota. A high proportion of isolates showed to be thermotolerant as they were detected at mesophilic and thermophilic phases. Isolated strains belonged to 187 bacterial species. Biodiversity was greater at the central stages of composting and mesophilic, thermophilic and cooling phases shared 50% of species.
authors.affiliation: Unit of Microbiology, Department of Biology and Geology, University of Almería, Agrifood Campus of International Excellence ceiA3, 04120 Almería, Spain.
authors.forename: J A
authors.initials: JA
authors.lastname: López-González
authors.affiliation: Unit of Microbiology, Department of Biology and Geology, University of Almería, Agrifood Campus of International Excellence ceiA3, 04120 Almería, Spain.
authors.forename: F
authors.initials: F
authors.lastname: Suárez-Estrella
authors.affiliation: Unit of Microbiology, Department of Biology and Geology, University of Almería, Agrifood Campus of International Excellence ceiA3, 04120 Almería, Spain.
authors.forename: M C
authors.initials: MC
authors.lastname: Vargas-García
authors.affiliation: Unit of Microbiology, Department of Biology and Geology, University of Almería, Agrifood Campus of International Excellence ceiA3, 04120 Almería, Spain.
authors.forename: M J
authors.initials: MJ
authors.lastname: López
authors.affiliation: Unit of Microbiology, Department of Biology and Geology, University of Almería, Agrifood Campus of International Excellence ceiA3, 04120 Almería, Spain.
authors.forename: M M
authors.initials: MM
authors.lastname: Jurado
authors.affiliation: Unit of Microbiology, Department of Biology and Geology, University of Almería, Agrifood Campus of International Excellence ceiA3, 04120 Almería, Spain. Electronic address: jcasco@ual.es.
authors.forename: J
authors.initials: J
authors.lastname: Moreno
mesh_terms: D001419:Bacteria / Q000235:genetics / Q000378:metabolism; D044822:Biodiversity; D004798:Enzymes / Q000378:metabolism; D008031:Lignin / Q000378:metabolism*; D064307:Microbiota / Q000502:physiology*; D012336:RNA, Ribosomal, 16S / Q000378:metabolism; D012987:Soil; D012988:Soil Microbiology
chemical_list: D004798:Enzymes; D012336:RNA, Ribosomal, 16S; D012987:Soil; C036909:lignocellulose; D008031:Lignin
keywords: Composting bacterial community; Composting biodiversity; Composting microbiome; Enzymatic functionality; Resident and transient microbiota