title: Canola (Brassica napus L.) NAC103 transcription factor gene is a novel player inducing reactive oxygen species accumulation and cell death in plants.
abstract: NAC transcription factors are plant-specific and play important roles in many processes including plant development, response to biotic and abiotic stresses and hormone signaling. So far, only a few NAC genes have been identified to mediate cell death. In this study, we identified a novel NAC gene from canola (Brassica napus L.), BnaNAC103 which induces reactive oxygen species (ROS) accumulation and cell death in Nicotianabenthamiana leaves. We found that BnaNAC103 responded to multiple signalings, including cold, salicylic acid (SA) and a fungal pathogen Sclerotinia sclerotiorum. BnaNAC103 is located in the nucleus. Expression of full-length BnaNAC103, but not either the N-terminal NAC domain or C-terminal regulatory domain, was identified to induce hypersensitive response (HR)-like cell death when expressed in N. benthamiana. The cell death triggered by BnaNAC103 is preceded by accumulation of ROS, with diaminobenzidine (DAB) staining supporting this. Moreover, quantification of ion leakage and malondialdehyde (MDA) of leaf discs indicates significant cell membrane breakage and lipid peroxidation induced by BnaNAC103 expression. Taken together, our work has identified a novel NAC transcription factor gene modulating ROS level and cell death in plants.
authors.affiliation: State Key Laboratory of Soil Erosion and Dryland Farming on Loess Plateau, College of Life Sciences, Northwest A&F University, Yangling, Shaanxi 712100, China. Electronic address: fangfangyouran@163.com.
authors.forename: Fangfang
authors.initials: F
authors.lastname: Niu
authors.affiliation: State Key Laboratory of Soil Erosion and Dryland Farming on Loess Plateau, College of Life Sciences, Northwest A&F University, Yangling, Shaanxi 712100, China. Electronic address: miao_kk@126.com.
authors.forename: Boya
authors.initials: B
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Soil Erosion and Dryland Farming on Loess Plateau, College of Life Sciences, Northwest A&F University, Yangling, Shaanxi 712100, China. Electronic address: 283915941@qq.com.
authors.forename: Feifei
authors.initials: F
authors.lastname: Wu
authors.affiliation: State Key Laboratory of Soil Erosion and Dryland Farming on Loess Plateau, College of Life Sciences, Northwest A&F University, Yangling, Shaanxi 712100, China. Electronic address: yanjingli512@163.com.
authors.forename: Jingli
authors.initials: J
authors.lastname: Yan
authors.affiliation: State Key Laboratory of Soil Erosion and Dryland Farming on Loess Plateau, College of Life Sciences, Northwest A&F University, Yangling, Shaanxi 712100, China. Electronic address: 994051374@qq.com.
authors.forename: Liang
authors.initials: L
authors.lastname: Li
authors.affiliation: State Key Laboratory of Soil Erosion and Dryland Farming on Loess Plateau, College of Life Sciences, Northwest A&F University, Yangling, Shaanxi 712100, China. Electronic address: wangchen361@sina.cn.
authors.forename: Chen
authors.initials: C
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Soil Erosion and Dryland Farming on Loess Plateau, College of Life Sciences, Northwest A&F University, Yangling, Shaanxi 712100, China. Electronic address: 859562712@qq.com.
authors.forename: Yiqiao
authors.initials: Y
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Soil Erosion and Dryland Farming on Loess Plateau, College of Life Sciences, Northwest A&F University, Yangling, Shaanxi 712100, China. Electronic address: yangwl@nwafu.edu.cn.
authors.forename: Bo
authors.initials: B
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Soil Erosion and Dryland Farming on Loess Plateau, College of Life Sciences, Northwest A&F University, Yangling, Shaanxi 712100, China. Electronic address: jiangyq@nwafu.edu.cn.
authors.forename: Yuan-Qing
authors.initials: YQ
authors.lastname: Jiang
mesh_terms: D000595:Amino Acid Sequence; D029688:Brassica napus / Q000166:cytology / Q000235:genetics* / Q000378:metabolism*; D016923:Cell Death / Q000235:genetics; D002467:Cell Nucleus / Q000378:metabolism; D003001:Cloning, Molecular; D017124:Conserved Sequence; D017343:Genes, Plant; D015227:Lipid Peroxidation; D008969:Molecular Sequence Data; D010802:Phylogeny; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D030821:Plants, Genetically Modified; D017382:Reactive Oxygen Species / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D015398:Signal Transduction; D013312:Stress, Physiological; D014026:Nicotiana / Q000235:genetics / Q000378:metabolism; D014157:Transcription Factors / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism
chemical_list: D010940:Plant Proteins; D017382:Reactive Oxygen Species; D011994:Recombinant Proteins; D014157:Transcription Factors
keywords: Brassica napus; Cell death; NAC; Reactive oxygen species; Transcription factor