title: MicroRNA-29b regulates migration in oral squamous cell carcinoma and its clinical significance.
abstract: OBJECTIVE
  MicroRNA (miRNA) machinery regulates cancer cell behavior, and has been implicated in patients' clinical status and prognosis. We found that microRNA-29b (miR-29b) increased significantly in advanced migratory cells. However, miR-29b controls the migration ability, and its regulatory mechanism in oral squamous cell carcinoma (OSCC) remains unknown.
  
  
  METHODS
  We triggered miR-29b expression in OSCC patients and cell lines by conducting real-time quantitative PCR. We determined the functions of miR-29b in the migration of OSCC cells by using gain- and loss-of-function approaches. We elevated the target genes of miR29b through software predictions and a luciferase report assay. We used an orthotopic OSCC animal model to investigate the effects of miR29b on OSCC cell metastasis in vivo.
  
  
  RESULTS
  The clinical data revealed that miR-29b expression was correlated with lymph node metastasis and an advanced tumor stage in 98 OSCC patients. Furthermore, multivariate analysis revealed that miR-29b expression was significantly correlated with recurrence, and indicated poor survival. MiR-29b promoted OSCC cell migration and downregulated CX3CL1, a cell-cell adhesion regulator, which plays an essential role in miR-29b-regulated OSCC cell migration machinery. Furthermore, we found that CX3CL1 expression was correlated with lymph node metastasis and an early tumor stage in OSCC patients, and negatively correlated with miR-29b expression.
  
  
  CONCLUSIONS
  MiR-29b acts as an oncomir, promoting cell migration through CX3CL1 suppression, and could be a potential therapeutic target for preventing OSCC progression.
authors.affiliation: Department of Otolaryngology, National Taiwan University Hospital, Taipei, Taiwan; Graduate Institute of Oral Biology, School of Dentistry, National Taiwan University, Taipei, Taiwan.
authors.forename: Cheng-Ning
authors.initials: CN
authors.lastname: Yang
authors.affiliation: Department of Dentistry, National Taiwan University Hospital Hsin-Chu Branch, Hsinchu, Taiwan.
authors.forename: Yi-Ting
authors.initials: YT
authors.lastname: Deng
authors.affiliation: Graduate Institute of Oral Biology, School of Dentistry, National Taiwan University, Taipei, Taiwan.
authors.forename: Jia-Yu
authors.initials: JY
authors.lastname: Tang
authors.affiliation: Department of Dentistry, National Taiwan University Hospital, Taipei, Taiwan.
authors.forename: Shih-Jung
authors.initials: SJ
authors.lastname: Cheng
authors.affiliation: Department of Pediatrics, National Taiwan University Hospital Yun-Lin Branch, Yunlin, Taiwan; Department of Pediatrics, National Taiwan University Hospital, Taipei, Taiwan.
authors.forename: Szu-Ta
authors.initials: ST
authors.lastname: Chen
authors.affiliation: Graduate Institute of Clinical Dentistry, School of Dentistry, National Taiwan University, Taipei, Taiwan.
authors.forename: Yue-Ju
authors.initials: YJ
authors.lastname: Li
authors.affiliation: Graduate Institute of Clinical Dentistry, School of Dentistry, National Taiwan University, Taipei, Taiwan.
authors.forename: Tai-Sheng
authors.initials: TS
authors.lastname: Wu
authors.affiliation: Institute of Clinical Medicine, National Yang-Ming University, Taipei, Taiwan.
authors.forename: Muh-Hwa
authors.initials: MH
authors.lastname: Yang
authors.affiliation: Department of Surgery, National Taiwan University Hospital, Taipei, Taiwan.
authors.forename: Been-Ren
authors.initials: BR
authors.lastname: Lin
authors.affiliation: Department of Dentistry, National Taiwan University Hospital, Taipei, Taiwan; Graduate Institute of Clinical Dentistry, School of Dentistry, National Taiwan University, Taipei, Taiwan.
authors.forename: Mark Yen-Ping
authors.initials: MY
authors.lastname: Kuo
authors.affiliation: Department of Otolaryngology, National Taiwan University Hospital, Taipei, Taiwan.
authors.forename: Jenq-Yuh
authors.initials: JY
authors.lastname: Ko
authors.affiliation: Graduate Institute of Oral Biology, School of Dentistry, National Taiwan University, Taipei, Taiwan; Department of Dentistry, National Taiwan University Hospital, Taipei, Taiwan; Angiogenesis Research Center, National Taiwan University, Taipei, Taiwan. Electronic address: ccclrc@gmail.com.
authors.forename: Cheng-Chi
authors.initials: CC
authors.lastname: Chang
mesh_terms: D000818:Animals; D002294:Carcinoma, Squamous Cell / Q000473:pathology*; D054428:Chemokine CX3CL1 / Q000235:genetics; D020868:Gene Silencing; D006801:Humans; D008207:Lymphatic Metastasis; D051379:Mice; D035683:MicroRNAs / Q000235:genetics / Q000502:physiology*; D009062:Mouth Neoplasms / Q000473:pathology*; D009362:Neoplasm Metastasis; D016019:Survival Analysis
chemical_list: D054428:Chemokine CX3CL1; C532045:MIRN29a microRNA, human; D035683:MicroRNAs
keywords: CX3CL1; Migration; Oral cancer; miR-29b