title: Effect of traditional Uyghur medicine abnormal Savda Munziq extract on rabbit platelet aggregation in vitro and rat arteriovenous shunt thrombosis in vivo.
abstract: OBJECTIVE
  Abnormal Savda Munziq (ASMq) is a standard herbal preparation used in Uyghur traditional medicine to fight chronic diseases including cardiovascular, but its specific effects on thrombosis and platelet aggregation are unknown.
  
  
  METHODS
  Rabbit platelets were incubated with ASMq extract (10, 20, 40 µg/ml) for 15, 30, or 60 min, and aggregation was induced with ADP and collagen. In vivo, ASMq extract 2.97 g/kg, 5.94g/kg, 11.88 g/kg per os daily for 15 days were tested on thrombus wet weight in a rat model of arterio-venous bypass thrombosis. Plasma thromboxane B2 (TXB2) and 6-keto-PGF1a (6PG) were measured by radioimmunoassay. Aspirin (12 µg/ml and 5mg/kg) and saline were used as control in both experiments.
  
  
  RESULTS
  ASMq inhibited ADP and collagen-induced aggregation in vitro in a dose-dependent manner that increased over time, to a maximum of 6.4 ± 1.3% and 21.6 ± 4.0% for ADP and collagen, respectively, at one hour׳s incubation with the highest concentration, whereas the effects of aspirin (34.5 ± 2.2% and 41.9 ± 2.5%, respectively) were stable over time. In vivo, ASMq inhibited thrombus formation dose-dependently, by 70% at the highest dose, compared to 67% with aspirin. ASMq essentially did not change prostaglandin production, compared to the clear inhibition by aspirin.
  
  
  CONCLUSIONS
  Abnormal Savda Munziq extract inhibits dose-dependent platelet aggregation with ADP or collagen in vitro and thrombosis in vivo to values similar to those of aspirin, though unlike aspirin this effect does not seem mediated by an inhibition of cyclo-oxygenase.
authors.affiliation: Department of Pharmacology, Xinjiang Medical University, 830054 Urumqi, Xinjiang, People׳s Republic of China; Department of Pharmacology, Universite de Bordeaux, F-33076 Bordeaux Cedex, France.
authors.forename: Anwar
authors.initials: A
authors.lastname: Umar
authors.affiliation: Department of Pharmacology, Xinjiang Medical University, 830054 Urumqi, Xinjiang, People׳s Republic of China.
authors.forename: Wuliya
authors.initials: W
authors.lastname: Yimin
authors.affiliation: Department of Pharmacology, Xinjiang Medical University, 830054 Urumqi, Xinjiang, People׳s Republic of China.
authors.forename: Ibadet
authors.initials: I
authors.lastname: Tohti
authors.affiliation: Department of Pharmacology, Xinjiang Medical University, 830054 Urumqi, Xinjiang, People׳s Republic of China. Electronic address: Halmurat@263.net.
authors.forename: Halmurat
authors.initials: H
authors.lastname: Upur
authors.affiliation: Department of Pharmacology, Universite de Bordeaux, F-33076 Bordeaux Cedex, France.
authors.forename: Bénédicte
authors.initials: B
authors.lastname: Berké
authors.affiliation: Department of Pharmacology, Xinjiang Medical University, 830054 Urumqi, Xinjiang, People׳s Republic of China; Department of Pharmacology, Universite de Bordeaux, F-33076 Bordeaux Cedex, France. Electronic address: nicholas.moore@pharmaco.u-bordeaux2.fr.
authors.forename: Nicholas
authors.initials: N
authors.lastname: Moore
mesh_terms: D000714:Anastomosis, Surgical; D000818:Animals; D002339:Carotid Arteries / Q000601:surgery; D007601:Jugular Veins / Q000601:surgery; D008297:Male; D008519:Medicine, Traditional; D010936:Plant Extracts / Q000494:pharmacology* / Q000627:therapeutic use*; D010974:Platelet Aggregation / Q000187:drug effects*; D010975:Platelet Aggregation Inhibitors / Q000494:pharmacology* / Q000627:therapeutic use*; D011817:Rabbits; D017207:Rats, Sprague-Dawley; D013927:Thrombosis / Q000188:drug therapy*
chemical_list: D010936:Plant Extracts; D010975:Platelet Aggregation Inhibitors; C000600923:abnormal Savda Munziq
keywords: Aspirin; Collagen; Extract of Abnormal Savda Munziq (ASMq); Platelet aggregation; Thrombosis; Traditional Uyghur medicine