title: Antithyroid drug detection using an enzyme cascade blocking in a nanoparticle-based lab-on-a-chip system.
abstract: A methimazole (MT) biosensor based on a nanocomposite of magnetic nanoparticles (MNPs) functionalized with iridium oxide nanoparticles (IrOx NPs) and tyrosinase (Tyr) immobilized onto screen printed electrode (SPE) by using a permanent magnet is presented. This system is evaluated in batch mode via chelating copper at the active site of tyrosinase and in flow mode by thioquinone formation. The MT detection in flow mode is achieved using a hybrid polydimethylsiloxane/polyester amperometric lab-on-a-chip (LOC) microsystem with an integrated SPE. Both systems are very sensitive with low limit of detection (LOD): 0.006 μM and 0.004 μM for batch and flow modes, respectively. Nevertheless, the flow mode has advantages such as its reusability, automation, low sample volume (6 μL), and fast response (20 s). Optimization and validation parameters such as enzyme-substrate amount, flow rate, inhibition conditions, repeatability and reproducibility of the biosensor have been performed. The proposed methods have been applied in MT detection in spiked human serum and pharmaceutical dosage forms.
authors.affiliation: Nanobioelectronics & Biosensors Group, ICN2- Institut Catala de Nanociencia i Nanotecnologia, Campus UAB, 08193 Bellaterra, Barcelona, Spain; Ankara University, Faculty of Pharmacy, Department of Analytical Chemistry, 06100 Tandogan, Ankara, Turkey.
authors.forename: Sevinc
authors.initials: S
authors.lastname: Kurbanoglu
authors.affiliation: Nanobioelectronics & Biosensors Group, ICN2- Institut Catala de Nanociencia i Nanotecnologia, Campus UAB, 08193 Bellaterra, Barcelona, Spain.
authors.forename: Carmen C
authors.initials: CC
authors.lastname: Mayorga-Martinez
authors.affiliation: Nanobioelectronics & Biosensors Group, ICN2- Institut Catala de Nanociencia i Nanotecnologia, Campus UAB, 08193 Bellaterra, Barcelona, Spain.
authors.forename: Mariana
authors.initials: M
authors.lastname: Medina-Sánchez
authors.affiliation: Nanobioelectronics & Biosensors Group, ICN2- Institut Catala de Nanociencia i Nanotecnologia, Campus UAB, 08193 Bellaterra, Barcelona, Spain.
authors.forename: Lourdes
authors.initials: L
authors.lastname: Rivas
authors.affiliation: Ankara University, Faculty of Pharmacy, Department of Analytical Chemistry, 06100 Tandogan, Ankara, Turkey.
authors.forename: Sibel A
authors.initials: SA
authors.lastname: Ozkan
authors.affiliation: Nanobioelectronics & Biosensors Group, ICN2- Institut Catala de Nanociencia i Nanotecnologia, Campus UAB, 08193 Bellaterra, Barcelona, Spain; ICREA, Barcelona, Catalonia, Spain. Electronic address: arben.merkoci@icn.cat.
authors.forename: Arben
authors.initials: A
authors.lastname: Merkoçi
mesh_terms: D013956:Antithyroid Agents / Q000737:chemistry / Q000302:isolation & purification*; D015374:Biosensing Techniques; D055664:Electrochemical Techniques; D004800:Enzymes, Immobilized / Q000737:chemistry; D006801:Humans; D007495:Iridium / Q000737:chemistry; D056656:Lab-On-A-Chip Devices; D057230:Limit of Detection; D046210:Microfluidic Analytical Techniques; D014442:Monophenol Monooxygenase / Q000737:chemistry; D053758:Nanoparticles / Q000737:chemistry*
chemical_list: D013956:Antithyroid Agents; D004800:Enzymes, Immobilized; C044458:iridium oxide; D007495:Iridium; D014442:Monophenol Monooxygenase
keywords: Enzyme inhibition; Iridium oxide nanoparticles; Lab-on-a-chip system; Magnetic nanoparticles; Methimazole detection