title: Selenium containing conducting polymer based pyranose oxidase biosensor for glucose detection.
abstract: A novel amperometric pyranose oxidase (PyOx) biosensor based on a selenium containing conducting polymer has been developed for the glucose detection. For this purpose, a conducting polymer; poly(4,7-bis(thieno[3,2-b]thiophen-2-yl)benzo[c][1,2,5] selenadiazole) (poly(BSeTT)) was synthesized via electropolymerisation on gold electrode to examine its matrix property for glucose detection. For this purpose, PyOx was used as the model enzyme and immobilised via physical adsorption technique. Amperometric detection of consumed oxygen was monitored at -0.7 V vs Ag reference electrode in a phosphate buffer (50 mM, pH 7.0). K(M)(app), Imax, LOD and sensitivity were calculated as 0.229 mM, 42.37 nA, 3.3 × 10(-4)nM and 6.4 nA/mM cm(2), respectively. Scanning electron microscopy (SEM), Electrochemical Impedance Spectroscopy (EIS) and cyclic voltammetry (CV) techniques were used to monitor changes in surface morphologies and to run electrochemical characterisations. Finally, the constructed biosensor was applied for the determination of glucose in beverages successfully.
authors.affiliation: Department of Chemistry, Middle East Technical University, Ankara 06800, Turkey. Electronic address: tugba.ceren@windowslive.com.
authors.forename: Tugba Ceren
authors.initials: TC
authors.lastname: Gokoglan
authors.affiliation: Department of Chemistry, Middle East Technical University, Ankara 06800, Turkey. Electronic address: saniyesoylemez@gmail.com.
authors.forename: Saniye
authors.initials: S
authors.lastname: Soylemez
authors.affiliation: Department of Chemistry, Middle East Technical University, Ankara 06800, Turkey. Electronic address: kesik.melis@gmail.com.
authors.forename: Melis
authors.initials: M
authors.lastname: Kesik
authors.affiliation: Department of Chemistry, Middle East Technical University, Ankara 06800, Turkey. Electronic address: sinemtoksabay@gmail.com.
authors.forename: Sinem
authors.initials: S
authors.lastname: Toksabay
authors.affiliation: Department of Chemistry, Middle East Technical University, Ankara 06800, Turkey; Department of Biotechnology, Middle East Technical University, Ankara 06800, Turkey; Department of Polymer Science and Technology, Middle East Technical University, Ankara 06800, Turkey; The Center for Solar Energy Research and Application (GUNAM), Middle East Technical University, Ankara 06800, Turkey. Electronic address: toppare@metu.edu.tr.
authors.forename: Levent
authors.initials: L
authors.lastname: Toppare
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D002237:Carbohydrate Dehydrogenases / Q000737:chemistry*; D004563:Electrochemistry; D005947:Glucose / Q000032:analysis*; D006046:Gold / Q000737:chemistry; D008855:Microscopy, Electron, Scanning; D011108:Polymers / Q000737:chemistry; D012643:Selenium / Q000737:chemistry*
chemical_list: D011108:Polymers; D006046:Gold; D002237:Carbohydrate Dehydrogenases; C022489:pyranose oxidase; D012643:Selenium; D005947:Glucose
keywords: Amperometric biosensor; Conducting polymer based biosensor; Glucose biosensor; Pyranose oxidase