title: Gelling polysaccharide from Chondrus armatus and its oligosaccharides: the structural peculiarities and anti-inflammatory activity.
abstract: Structural peculiarities of low molecular weight (LMW) sample obtained by mild acid hydrolysis of κ-carrageenan from Chondrus armatus was investigated by a rapid mass spectrometric method. The selected conditions allowed avoiding excess destruction of 3,6-AnGal residues that was shown by using tandem (MS/MS) mode. Main oligosaccharide fraction with molecular weight 2.3 kg/mol, obtained by mild acid hydrolysis was chosen for the analysis. It was shown that fragments with even degree of polymerization (DP) were mostly built of (-G4S-DA-)n repeating unit, n=1-5. Some fragments with odd DP were shown to contain -DA2S- insertions, being the fragments of ι-type blocks, which were randomly distributed along the polysaccharide chain as single insertions. The anti-inflammatory activity (on acetic acid-induced colitis in mice) of the initial polymer and its derivatives was studied. The anti-inflammatory effect of the polymer was observed at a dose of 5mg/kg. Polysaccharide decreased the degree of colon damage more than twice and area of damage in 40%.
authors.affiliation: G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch, Russian Academy of Sciences, 100 Let Vladivostoku Prosp., 159, 690022 Vladivostok, Russian Federation. Electronic address: kalitnik85@mail.ru.
authors.forename: Alexandra A
authors.initials: AA
authors.lastname: Kalitnik
authors.affiliation: Institute of Physiology, Komi Science Centre, The Urals Branch, Russian Academy of Sciences, Syktyvkar, Russian Federation.
authors.forename: Pavel A
authors.initials: PA
authors.lastname: Marcov
authors.affiliation: G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch, Russian Academy of Sciences, 100 Let Vladivostoku Prosp., 159, 690022 Vladivostok, Russian Federation.
authors.forename: Stanislav D
authors.initials: SD
authors.lastname: Anastyuk
authors.affiliation: G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch, Russian Academy of Sciences, 100 Let Vladivostoku Prosp., 159, 690022 Vladivostok, Russian Federation.
authors.forename: Anna O Byankina
authors.initials: AO
authors.lastname: Barabanova
authors.affiliation: G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch, Russian Academy of Sciences, 100 Let Vladivostoku Prosp., 159, 690022 Vladivostok, Russian Federation.
authors.forename: Valeriy P
authors.initials: VP
authors.lastname: Glazunov
authors.affiliation: Institute of Physiology, Komi Science Centre, The Urals Branch, Russian Academy of Sciences, Syktyvkar, Russian Federation.
authors.forename: Sergey V
authors.initials: SV
authors.lastname: Popov
authors.affiliation: Institute of Physiology, Komi Science Centre, The Urals Branch, Russian Academy of Sciences, Syktyvkar, Russian Federation.
authors.forename: Yuriy S
authors.initials: YS
authors.lastname: Ovodov
authors.affiliation: G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch, Russian Academy of Sciences, 100 Let Vladivostoku Prosp., 159, 690022 Vladivostok, Russian Federation.
authors.forename: Irina M
authors.initials: IM
authors.lastname: Yermak
mesh_terms: D019342:Acetic Acid; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use; D002351:Carrageenan / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use; D044723:Chondrus; D003092:Colitis / Q000139:chemically induced / Q000188:drug therapy* / Q000201:enzymology / Q000473:pathology; D003106:Colon / Q000187:drug effects / Q000201:enzymology / Q000473:pathology; D005782:Gels; D006868:Hydrolysis; D008297:Male; D051379:Mice; D008970:Molecular Weight; D009844:Oligosaccharides / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use; D009195:Peroxidase / Q000378:metabolism; D053719:Tandem Mass Spectrometry
chemical_list: D000893:Anti-Inflammatory Agents; D005782:Gels; D009844:Oligosaccharides; D002351:Carrageenan; D009195:Peroxidase; D019342:Acetic Acid
keywords: Acid-induced colitis; Inflammatory; Mild acid hydrolysis; Myeloperoxidase; Oligosaccharides; κ-Carrageenan