title: PRDI-BF1-RIZ domain of retinoblastoma protein-interacting zinc finger gene 1 induces apoptosis and exerts anticancer activity in esophageal squamous cell carcinoma cells.
abstract: The present study examined the role of the PRDI-BF1-RIZ (PR) domain of tumor suppressor retinoblastoma protein-interacting zinc finger gene 1 (RIZ1) as an anticancer domain and its ability to induce apoptosis in esophageal squamous cell carcinoma (ESCC) cells. The TE13 ESCC cell line was transfected with pcDNA3.1(+) eukaryotic expression vectors bearing the open reading frames of either the humanRIZ1gene or the PR domain, and the mRNA and protein expression levels were then detected using quantitative reverse transcription polymerase chain reaction and western blotting, respectively. The rate of apoptosis was determined by flow cytometry and the cell invasion ability was determined by an invasion assay. RIZ1 and the PR domain induced apoptosis and reduced the cell invasion ability (P<0.01). These findings indicate that theRIZ1gene possesses anticancer activity in the PR domain, which may be important in inhibiting the development of ESCC.
authors.affiliation: Department of Cardiothoracic Surgery, Tianjin Medical University General Hospital, Tianjin 300052, P.R. China.
authors.forename: Shang-Wen
authors.initials: SW
authors.lastname: Dong
authors.affiliation: First Department of Radiotherapy, Henan Province Anyang Tumor Hospital, Anyang, Henan 455000, P.R. China.
authors.forename: Yao-Wen
authors.initials: YW
authors.lastname: Zhang
authors.affiliation: Department of Cardiothoracic Surgery, Tianjin Medical University General Hospital, Tianjin 300052, P.R. China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Department of Cardiothoracic Surgery, Tianjin Medical University General Hospital, Tianjin 300052, P.R. China.
authors.forename: Shuo
authors.initials: S
authors.lastname: Wang
authors.affiliation: Tianjin Institute of Endocrinology, Tianjin Medical University, Tianjin 300070, P.R. China.
authors.forename: Pei
authors.initials: P
authors.lastname: Sun
authors.affiliation: Department of Cardiothoracic Surgery, Tianjin Medical University General Hospital, Tianjin 300052, P.R. China.
authors.forename: Yuan-Guo
authors.initials: YG
authors.lastname: Wang
authors.affiliation: Department of Cardiothoracic Surgery, Tianjin Medical University General Hospital, Tianjin 300052, P.R. China.
authors.forename: Peng
authors.initials: P
authors.lastname: Zhang
keywords: PRDI-BF1-RIZ domain; esophageal squamous cell carcinoma; retinoblastoma protein-interacting zinc finger gene 1