title: Clastogenic effects of Trypanosoma brucei brucei and Trypanosoma evansi mixed infection in bone marrow of Wistar rats.
abstract: The clastogenic effect of mixed infection of Trypanosoma evansi and Trypanosoma brucei brucei in the bone marrow (BM) cells of Wistar albino rats was investigated. Clastogenic effects were observed in the BM cells using the micronucleus assay. The findings indicate that T. evansi, T. b. brucei and mixed infection with both parasites induced the formation of micronucleated polychromatic erythrocyte (MN-PCEs) in the BM cells significantly (P < 0.05) by 60, 63 and 81 micronuclei/1000 PCE respectively. Mixed infection induced formation of MN-PCEs increase by about 1.33 fold when compared with single infections of T. b. brucei and T. evansi. These data give a preliminary evidence of possible genotoxic effects in trypanosomiasis.
authors.affiliation: Department of Biochemistry, Ahmadu Bello University Zaria, Kaduna State 810001, Nigeria; Department of Biochemistry and Molecular Biology, Monash University, Clayton, Vic. 3800, Australia. Electronic address: nathanhabila@gmail.com.
authors.forename: Nathan
authors.initials: N
authors.lastname: Habila
authors.affiliation: Department of Biochemistry, Ahmadu Bello University Zaria, Kaduna State 810001, Nigeria.
authors.forename: Aliyu
authors.initials: A
authors.lastname: Muhammad
authors.affiliation: Department of Biochemistry, Ahmadu Bello University Zaria, Kaduna State 810001, Nigeria.
authors.forename: Uche S
authors.initials: US
authors.lastname: Ndidi
authors.affiliation: Department of Biochemistry, Ahmadu Bello University Zaria, Kaduna State 810001, Nigeria.
authors.forename: Gloria D
authors.initials: GD
authors.lastname: Chechet
authors.affiliation: Department of Biochemistry, Ahmadu Bello University Zaria, Kaduna State 810001, Nigeria.
authors.forename: Idowu A
authors.initials: IA
authors.lastname: Aimola
authors.affiliation: Department of Biochemistry, Ahmadu Bello University Zaria, Kaduna State 810001, Nigeria.
authors.forename: Bello A
authors.initials: BA
authors.lastname: Olalekan
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D018053:Arsenites / Q000633:toxicity; D001853:Bone Marrow / Q000469:parasitology*; D060085:Coinfection / Q000235:genetics* / Q000469:parasitology*; D004249:DNA Damage; D004912:Erythrocytes / Q000469:parasitology / Q000473:pathology*; D008297:Male; D015162:Micronucleus Tests; D016296:Mutagenesis; D018512:Parasitemia; D051381:Rats; D017208:Rats, Wistar; D017670:Sodium Compounds / Q000633:toxicity; D014346:Trypanosoma brucei brucei / Q000472:pathogenicity*; D014353:Trypanosomiasis, African / Q000235:genetics* / Q000469:parasitology*
chemical_list: D018053:Arsenites; D017670:Sodium Compounds; C017947:sodium arsenite
keywords: Bone marrow; Clastogenenicity; Micro nucleus assay; Micronucleated polychromatic erythrocyte; Trypanosoma brucei brucei; Trypanosoma evansi