title: Solubilization and refolding of inclusion body proteins.
abstract: High-level expression of recombinant proteins in Escherichia coli often results in accumulation of protein molecules into aggregates known as inclusion bodies (IBs). Isolation of properly folded, bioactive protein from IBs is a cumbersome task and most of the times results in poor recovery. The process of recovering bioactive proteins from IBs consists of solubilization of IB aggregates using denaturants, followed by refolding of the solubilized protein. Here, we describe a simple protocol for screening of buffers for solubilization of IB proteins. Various IB aggregate solubilization methods including organic solvents have been described.
authors.affiliation: Product Development Cell, National Institute of Immunology, Aruna Asaf Ali Marg, New Delhi, 110067, India.
authors.forename: Anupam
authors.initials: A
authors.lastname: Singh
authors.forename: Vaibhav
authors.initials: V
authors.lastname: Upadhyay
authors.forename: Amulya K
authors.initials: AK
authors.lastname: Panda
mesh_terms: D000818:Animals; D002021:Buffers; D004926:Escherichia coli / Q000378:metabolism; D006801:Humans; D002479:Inclusion Bodies / Q000378:metabolism*; D017510:Protein Folding; D011994:Recombinant Proteins / Q000378:metabolism*; D012995:Solubility
chemical_list: D002021:Buffers; D011994:Recombinant Proteins