title: Genetic characterization of the Makrani people of Pakistan from mitochondrial DNA control-region data.
abstract: To estimate genetic and forensic parameters, the entire mitochondrial DNA control region of 100 unrelated Makrani individuals (males, n=96; females, n=4) living in Pakistan (Turbat, Panjgur, Awaran, Kharan, Nasirabad, Gwadar, Buleda, Karachi and Burewala) was sequenced. We observed a total of 70 different haplotypes of which 54 were unique and 16 were shared by more than one individual. The Makrani population showed a high genetic diversity (0.9688) and, consequently, a high power of discrimination (0.9592). Our results revealed a strongly admixed mtDNA pool composed of African haplogroups (28%), West Eurasian haplogroups (26%), South Asian haplogroups (24%), and East Asian haplogroups (2%), while the origin of the remaining individuals (20%) could not be confidently assigned. The results of this study are a valuable contribution to build a database of mtDNA variation in Pakistan.
authors.affiliation: Department of Zoology, University of the Punjab, Lahore 54590, Pakistan. Electronic address: mhassansiddiqimg@gmail.com.
authors.forename: Muhammad Hassan
authors.initials: MH
authors.lastname: Siddiqi
authors.affiliation: Department of Zoology, University of the Punjab, Lahore 54590, Pakistan.
authors.forename: Tanveer
authors.initials: T
authors.lastname: Akhtar
authors.affiliation: Department of Forensic Sciences, University of Health Sciences, Lahore 54590, Pakistan.
authors.forename: Allah
authors.initials: A
authors.lastname: Rakha
authors.affiliation: Department of Zoology, University of the Punjab, Lahore 54590, Pakistan.
authors.forename: Ghazanfar
authors.initials: G
authors.lastname: Abbas
authors.affiliation: Department of Biotechnology and Informatics, Baluchistan University of Information Technology, Engineering and Management Sciences (BUITEMS), Quetta 78300, Pakistan.
authors.forename: Akram
authors.initials: A
authors.lastname: Ali
authors.affiliation: Department of Zoology, University of the Punjab, Lahore 54590, Pakistan.
authors.forename: Naeem
authors.initials: N
authors.lastname: Haider
authors.affiliation: Institute of Molecular Biology & Biotechnology, The University of Lahore, 54590, Pakistan.
authors.forename: Azam
authors.initials: A
authors.lastname: Ali
authors.affiliation: Department of Zoology, University of the Punjab, Lahore 54590, Pakistan.
authors.forename: Sikandar
authors.initials: S
authors.lastname: Hayat
authors.affiliation: Department of Zoology, University of the Punjab, Lahore 54590, Pakistan.
authors.forename: Syeda
authors.initials: S
authors.lastname: Masooma
authors.affiliation: Department of Biotechnology and Informatics, Baluchistan University of Information Technology, Engineering and Management Sciences (BUITEMS), Quetta 78300, Pakistan.
authors.forename: Jamil
authors.initials: J
authors.lastname: Ahmad
authors.affiliation: Department of Biosciences, COMSATS Institute of Information Technology, Sahiwal, Pakistan.
authors.forename: Muhammad Akram
authors.initials: MA
authors.lastname: Tariq
authors.affiliation: Department of Forensic Molecular Biology, Erasmus MC - University Medical Center Rotterdam, The Netherlands.
authors.forename: Mannis
authors.initials: M
authors.lastname: van Oven
authors.affiliation: Department of Zoology, University of the Punjab, Lahore 54590, Pakistan.
authors.forename: Fazle Majid
authors.initials: FM
authors.lastname: Khan
mesh_terms: D044466:Asian People / Q000235:genetics*; D004272:DNA, Mitochondrial / Q000235:genetics*; D005260:Female; D014644:Genetic Variation; D006239:Haplotypes; D006801:Humans; D008297:Male; D010154:Pakistan
chemical_list: D004272:DNA, Mitochondrial
keywords: Haplotyping; Hypervariable segment (HVS); Makrani population; Mitochondrial DNA; Pakistan; mtDNA control region