title: Transcriptional regulation and functional involvement of the Arabidopsis pescadillo ortholog AtPES in root development.
abstract: The Pescadillo gene is highly conserved from yeasts to human and has been shown to impact on both the cell cycle and on ribosome biogenesis. However, the biological function and transcriptional regulation of the plant orthologs remain unclear. In the present study, we have implemented a combination of molecular and genetic approaches, in order to characterize the Arabidopsis thaliana pescadillo ortholog (AtPES) and its role in root development. The RNAi transgenic lines displayed severely compromised meristem structures and a reduction of the primary root length of up to 70%. The correct pattern of the cell files is distorted, whereas in the root elongation and differentiation zone the epidermal and cortex cells appear abnormally enlarged. Yeast two hybrid and BiFC experiments confirmed that AtPES interacts physically with AtPEIP1 and AtPEIP2, the orthologs of the murine Bop1 and WDR12. Promoter deletion analysis revealed that AtPES expression depends on a number of transcription factor binding sites, with the TELO-box being a crucial site for regulating its accurate tissue-specific manifestation. Our results indicate that AtPES is firmly regulated at the transcriptional level and that the corresponding protein plays a role in root developmental processes.
authors.affiliation: University of Athens, Faculty of Biology, Department of Botany, 15784 Athens, Greece. Electronic address: aristzo@yahoo.gr.
authors.forename: Aris
authors.initials: A
authors.lastname: Zografidis
authors.affiliation: University of Athens, Faculty of Biology, Department of Botany, 15784 Athens, Greece. Electronic address: gkapolas@yahoo.gr.
authors.forename: Giorgos
authors.initials: G
authors.lastname: Kapolas
authors.affiliation: University of Athens, Faculty of Biology, Department of Botany, 15784 Athens, Greece. Electronic address: vapodia@hotmail.com.
authors.forename: Varvara
authors.initials: V
authors.lastname: Podia
authors.affiliation: University of Athens, Faculty of Biology, Department of Botany, 15784 Athens, Greece. Electronic address: debthem@hotmail.com.
authors.forename: Despoina
authors.initials: D
authors.lastname: Beri
authors.affiliation: University of Thessaly, Department of Biochemistry & Biotechnology, 41221 Larissa, Greece. Electronic address: kalpapad@bio.uth.gr.
authors.forename: Kalliope
authors.initials: K
authors.lastname: Papadopoulou
authors.affiliation: Agricultural University of Athens, Department of Agricultural Biotechnology, 11855 Athens, Greece. Electronic address: bmbi2mid@aua.gr.
authors.forename: Dimitra
authors.initials: D
authors.lastname: Milioni
authors.affiliation: University of Athens, Faculty of Biology, Department of Botany, 15784 Athens, Greece. Electronic address: kharalamp@biol.uoa.gr.
authors.forename: Kosmas
authors.initials: K
authors.lastname: Haralampidis
mesh_terms: D000818:Animals; D017360:Arabidopsis / Q000235:genetics* / Q000254:growth & development*; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D001665:Binding Sites; D018797:Cell Cycle Proteins / Q000235:genetics* / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D005966:Glucuronidase / Q000378:metabolism; D051379:Mice; D008969:Molecular Sequence Data; D010641:Phenotype; D018517:Plant Roots / Q000235:genetics* / Q000254:growth & development*; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D025941:Protein Interaction Mapping; D034622:RNA Interference; D036226:Seedlings / Q000235:genetics; D017384:Sequence Deletion; D017386:Sequence Homology, Amino Acid; D014158:Transcription, Genetic
chemical_list: D029681:Arabidopsis Proteins; D018797:Cell Cycle Proteins; C000588608:AT5G14520 protein, Arabidopsis; D005966:Glucuronidase
keywords: Arabidopsis thaliana; Nucleolus; Pescadillo; Promoter analysis; RNAi