title: Intracellular synthesis of silver nanoparticle by actinobacteria and its antimicrobial activity.
abstract: Intracellular synthesis of silver nanoparticles (AgNPs) using Rhodococcus spp. is demonstrated. The synthesized nanoparticles were characterized by UV-Vis spectroscopy, X-ray diffraction, energy dispersive spectroscopy, Fourier trans-form infrared spectroscopy, and transmission electron microscopy. Transmission electron microscopy study of microorganisms' revealed synthesis of nanoparticle was occurring inside the cell, in the cytoplasm. AgNPs ranged from 5 to 50 nm. Formed nanoparticles were stable in the colloidal solution due to presence of proteins on the surface. AgNPs showed excellent bactericidal and bacteriostatic activity against pathogenic microorganisms.
authors.affiliation: Center for Interdisciplinary Research, D.Y. Patil University, Kolhapur 416 006, Maharashtra State, India.
authors.forename: S V
authors.initials: SV
authors.lastname: Otari
authors.affiliation: Center for Interdisciplinary Research, D.Y. Patil University, Kolhapur 416 006, Maharashtra State, India.
authors.forename: R M
authors.initials: RM
authors.lastname: Patil
authors.affiliation: Center for Interdisciplinary Research, D.Y. Patil University, Kolhapur 416 006, Maharashtra State, India.
authors.forename: S J
authors.initials: SJ
authors.lastname: Ghosh
authors.affiliation: Center for Interdisciplinary Research, D.Y. Patil University, Kolhapur 416 006, Maharashtra State, India; Samsung Biomedical Research Institute, Department of Molecular Cell Biology, Sungkyunkwan University School of Medicine, Suwon 440-746, South Korea. Electronic address: thoratnd@yahoo.com.
authors.forename: N D
authors.initials: ND
authors.lastname: Thorat
authors.affiliation: Center for Interdisciplinary Research, D.Y. Patil University, Kolhapur 416 006, Maharashtra State, India. Electronic address: pawar_s_h@yahoo.com.
authors.forename: S H
authors.initials: SH
authors.lastname: Pawar
mesh_terms: D039903:Actinobacteria / Q000378:metabolism*; D000890:Anti-Infective Agents / Q000494:pharmacology*; D042541:Intracellular Space / Q000378:metabolism*; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D008826:Microbial Sensitivity Tests; D012834:Silver / Q000494:pharmacology*; D013052:Spectrometry, X-Ray Emission; D013056:Spectrophotometry, Ultraviolet
chemical_list: D000890:Anti-Infective Agents; D012834:Silver
keywords: Actinobacteria; AgNPs; DLS; Green synthesis; Intracellular synthesis