title: A novel solid phase extraction--ultra high performance liquid chromatography-tandem mass spectrometry method for the quantification of ochratoxin A in red wines.
abstract: A novel and advanced technology on solid phase extraction column prior to liquid chromatography coupled to tandem mass spectrometry has been used for the determination of ochratoxin A in red wine samples. Due to the need of a reliable and rugged method according to current regulations and with the aim of minimize heuristic efforts associated with analytical method development, the statistical design of experiment was employed. On other hand, the method validation according to European Commission 2002/657/EC was achieved. The values obtained for decision limit (CCα), detection capability (CCβ), limits of detection and quantification were 0.07 μg L(-1), 0.14 μg L(-1), 0.13 μg L(-1) and 0.41 μg L(-1), respectively. The recoveries values were ranged from 95.7% to 107.2%. These values were compatible with the 2.0 μg L(-1) maximum allowable concentration limit established by different international regulations.
authors.affiliation: Instituto de Química de San Luis (CONICET-UNSL), Laboratorio de Espectrometría de Masas, Bloque III, Avda. Ejército de los Andes 950, San Luis, Argentina.
authors.forename: Leonardo
authors.initials: L
authors.lastname: Mariño-Repizo
authors.affiliation: Biotage, 10430 Harris Oaks Blvd., Suite C, Charlotte, NC 28269, USA.
authors.forename: Frank
authors.initials: F
authors.lastname: Kero
authors.affiliation: Biotage, 10430 Harris Oaks Blvd., Suite C, Charlotte, NC 28269, USA.
authors.forename: Victor
authors.initials: V
authors.lastname: Vandell
authors.affiliation: Biotage, 10430 Harris Oaks Blvd., Suite C, Charlotte, NC 28269, USA.
authors.forename: Adam
authors.initials: A
authors.lastname: Senior
authors.affiliation: Instituto de Química de San Luis (CONICET-UNSL), Laboratorio de Espectrometría de Masas, Bloque III, Avda. Ejército de los Andes 950, San Luis, Argentina.
authors.forename: M
authors.initials: M
authors.lastname: Isabel Sanz-Ferramola
authors.affiliation: Instituto de Química de San Luis (CONICET-UNSL), Laboratorio de Espectrometría de Masas, Bloque III, Avda. Ejército de los Andes 950, San Luis, Argentina. Electronic address: ecerutti@gmail.com.
authors.forename: Soledad
authors.initials: S
authors.lastname: Cerutti
authors.affiliation: Instituto de Química de San Luis (CONICET-UNSL), Laboratorio de Espectrometría de Masas, Bloque III, Avda. Ejército de los Andes 950, San Luis, Argentina.
authors.forename: Julio
authors.initials: J
authors.lastname: Raba
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005506:Food Contamination / Q000032:analysis*; D005516:Food Microbiology; D009793:Ochratoxins / Q000032:analysis*; D015203:Reproducibility of Results; D052616:Solid Phase Extraction / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods*; D014920:Wine / Q000032:analysis*
chemical_list: D009793:Ochratoxins; C025589:ochratoxin A
keywords: Ochratoxin A; Red wine; Solid phase extraction; UPLC–MS/MS