title: Lignocellulose degradation by the isolate of Streptomyces griseorubens JSD-1.
abstract: Streptomyces griseorubens JSD-1 is an isolate that can utilize lignocellulose (straw) as its sole carbon source for growth, and these lignocellulolytic genes involved in this biotransformation are expected to be crucial. However, little is known about the genetic basis related to this process. To further investigate the lignocellulose-degrading mechanisms, genome sequencing was carried out using MiSeq platform. After obtaining its draft genome, the key lignocellulolytic genes such as multicopper oxidase, exo-1, 4-β-glucanase, endo-1,4-β-glucanase, and β-xylosidase were identified and characterized. Multiple sequence alignments were performed to find out the identities of these analyzed proteins to those of their similar species. Signal peptide cleavage sites were predicted by SignalP 4.1 to reflect the cellular localization of their mature proteins. Besides, predicted 3D structures of these proteins were modeled by Phyre2, which showed to be highly identical to the templates in the PDB database. Finally, quantitative real-time PCR revealed that expression levels of all analyzed enzymes were significantly and generally up-regulated during the whole cultivation, indicating that they all contributed to the biodegradation.
authors.affiliation: School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, 200240, China.
authors.forename: Haiwei
authors.initials: H
authors.lastname: Feng
authors.forename: Yujing
authors.initials: Y
authors.lastname: Sun
authors.forename: Yuee
authors.initials: Y
authors.lastname: Zhi
authors.forename: Liang
authors.initials: L
authors.lastname: Mao
authors.forename: Yanqing
authors.initials: Y
authors.lastname: Luo
authors.forename: Xing
authors.initials: X
authors.lastname: Wei
authors.forename: Pei
authors.initials: P
authors.lastname: Zhou
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D016680:Genome, Bacterial; D008031:Lignin / Q000378:metabolism*; D008958:Models, Molecular; D016415:Sequence Alignment; D013302:Streptomyces / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; C036909:lignocellulose; D008031:Lignin