title: Label-free selection and enrichment of liver cancer stem cells by surface niches build up with polyelectrolyte multilayer films.
abstract: Recent studies indicate that a small population of cancer cells exhibits stem cell properties and are referred to as cancer-initiating or cancer stem cells (CSCs). The selection and identification of cancer stem cells through methods require well-defined biomarkers and immunolabeling procedures are complicated and often unreliable. Herein, we fabricated a series of microenviroment by using polyelectrolyte multilayers (PEM) nanofilms to program and mimic hepatocellular carcinoma CSCs niches for CSCs selection with a label-free method. When cultured on PEM substrates, human cancer cell lines-Huh7 cells grew into individual round colonies and these cells displayed high marker expression of CSCs. Especially, these selected cells demonstrated significant chemo-resistant property in comparison with normal population. Therefore, we believed that niches selection and colony formation method may provide a new strategy on CSCs selection and drug evaluation for cancer therapy.
authors.affiliation: Graduate Institute of Biochemical and Biomedical Engineering, Chang Gung University, Taoyuan, Taiwan. Electronic address: iclee@mail.cgu.edu.tw.
authors.forename: I-Chi
authors.initials: IC
authors.lastname: Lee
authors.affiliation: Graduate Institute of Biochemical and Biomedical Engineering, Chang Gung University, Taoyuan, Taiwan.
authors.forename: Jen-Fu
authors.initials: JF
authors.lastname: Chang
mesh_terms: D000903:Antibiotics, Antineoplastic / Q000494:pharmacology; D014408:Biomarkers, Tumor / Q000378:metabolism*; D045744:Cell Line, Tumor; D002469:Cell Separation / Q000379:methods; D002470:Cell Survival / Q000187:drug effects; D004317:Doxorubicin / Q000494:pharmacology; D019008:Drug Resistance, Neoplasm; D015870:Gene Expression; D022781:Hepatocytes / Q000187:drug effects / Q000378:metabolism / Q000473:pathology*; D006801:Humans; D049329:Nanostructures / Q000737:chemistry*; D014411:Neoplastic Stem Cells / Q000187:drug effects / Q000378:metabolism / Q000473:pathology*; D011073:Polyamines / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D055672:Static Electricity; D055153:Stem Cell Niche; D013451:Sulfonic Acids / Q000737:chemistry*
chemical_list: D000903:Antibiotics, Antineoplastic; D014408:Biomarkers, Tumor; D011073:Polyamines; D011108:Polymers; D013451:Sulfonic Acids; C063994:polyallylamine; D004317:Doxorubicin; C077114:styrenesulfonic acid polymer
keywords: Cancer stem cells; Chemo-resistant; Label-free selection; Polyelectrolyte multilayers films; Stem cell niches