title: Biological and biochemical properties of two Xenopus laevis N-acetylgalactosaminyltransferases with contrasting roles in embryogenesis.
abstract: The biosynthesis of mucin-type O-linked glycans in animals is initiated by members of the large family of polypeptide N-acetylgalactosaminyltransferases (GalNAc-Ts), which play important roles in embryogenesis, organogenesis, adult tissue homeostasis and carcinogenesis. Until now, the mammalian forms of these enzymes have been the best characterized. However, two N-acetylgalactosaminyltransferases (xGalNAc-T6 and xGalNAc-T16) from the African clawed frog (Xenopus laevis), which are most homologous to those encoded by the human GALNT6 and GALNT16 (GALNTL1) genes, were shown to have contrasting roles in TGF-β/BMP signaling in embryogenesis. In this study we have examined these two enzymes further and show differences in their in vivo function during X. laevis embyrogenesis as evidenced by in situ hybridization and overexpression experiments. In terms of enzymatic activity, both enzymes were found to be active towards the EA2 peptide, but display differential activity towards a peptide based on the sequence of ActR-IIB, a receptor relevant to TGF-β/BMP signaling. In summary, these data demonstrate that these two enzymes from different branches of the N-acetylgalactosaminyltransferase do not only display differential substrate specificities, but also specific and distinct expression pattern and biological activities in vivo.
authors.affiliation: Department für Chemie, Universität für Bodenkultur, Wien, Austria; Manchester Interdisciplinary Biocentre, University of Manchester, UK.
authors.forename: Josef
authors.initials: J
authors.lastname: Voglmeir
authors.affiliation: Manchester Interdisciplinary Biocentre, University of Manchester, UK.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Laurent
authors.affiliation: Manchester Interdisciplinary Biocentre, University of Manchester, UK.
authors.forename: Sabine L
authors.initials: SL
authors.lastname: Flitsch
authors.affiliation: Max-Planck-Institut für Immunbiologie, Freiburg, Germany.
authors.forename: Michael
authors.initials: M
authors.lastname: Oelgeschläger
authors.affiliation: Department für Chemie, Universität für Bodenkultur, Wien, Austria. Electronic address: iain.wilson@boku.ac.at.
authors.forename: Iain B H
authors.initials: IB
authors.lastname: Wilson
mesh_terms: D000818:Animals; D004625:Embryo, Nonmammalian / Q000201:enzymology; D047108:Embryonic Development; D006801:Humans; D006863:Hydrogen-Ion Concentration; D017350:N-Acetylgalactosaminyltransferases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D020033:Protein Isoforms / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction; D013379:Substrate Specificity; D014982:Xenopus laevis / Q000196:embryology / Q000235:genetics / Q000378:metabolism*; D000097763:Polypeptide N-acetylgalactosaminyltransferase
chemical_list: D017350:N-Acetylgalactosaminyltransferases; D020033:Protein Isoforms; D000097763:Polypeptide N-acetylgalactosaminyltransferase
keywords: Development; Mucin; N-acetylgalactosaminyltransferase