title: Proteomic analysis of changes in the protein composition of MCF-7 human breast cancer cells induced by all-trans retinoic acid, 9-cis retinoic acid, and their combination.
abstract: Retinoic acid (all-trans and 9-cis) isomers represent important therapeutic agents for many types of cancers, including human breast cancer. Changes in protein composition of the MCF-7 human breast cancer cells were induced by all-trans retinoic acid, 9-cis retinoic acid, and their combination and subsequently proteomic strategies based on bottom-up method were applied. Proposed approach was used for the analysis of proteins extracted from MCF-7 human breast cancer cell line utilizing a commercially manufactured kit RIPA and separated on two dimensional (2D) sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) after treatment with both retinoic acid isomers. We found significant differences in occurrence of proteins probably affecting the cell migration process in tumour cells. Heat shock protein 27, ribonucleoprotein SmD3, and cofilin-1 were significantly upregulated after treatment with combination of individual retinoic acid isomers. On the other hand, AP-5 complex subunit beta-1 shows the different response. Thus, the results might help to find the answer to important medical questions on (i) the identification of signaling pathways affected by retinoic acid isomers or (ii) how the observed proteomic pattern might reflect the effectiveness of retinoic acids treatment.
authors.affiliation: Institute of Analytical Chemistry of the ASCR, v. v. i., Brno, Czech Republic.
authors.forename: D
authors.initials: D
authors.lastname: Flodrova
authors.affiliation: Institute of Analytical Chemistry of the ASCR, v. v. i., Brno, Czech Republic.
authors.forename: D
authors.initials: D
authors.lastname: Benkovska
authors.affiliation: Institute of Experimental Endocrinology, Slovak Academy of Sciences, Bratislava, Slovak Republic.
authors.forename: D
authors.initials: D
authors.lastname: Macejova
authors.affiliation: Institute of Experimental Endocrinology, Slovak Academy of Sciences, Bratislava, Slovak Republic.
authors.forename: L
authors.initials: L
authors.lastname: Bialesova
authors.affiliation: Institute of Experimental Oncology, Slovak Academy of Sciences, Bratislava, Slovak Republic.
authors.forename: L
authors.initials: L
authors.lastname: Hunakova
authors.affiliation: Institute of Experimental Endocrinology, Slovak Academy of Sciences, Bratislava, Slovak Republic.
authors.forename: J
authors.initials: J
authors.lastname: Brtko
authors.affiliation: Institute of Analytical Chemistry of the ASCR, v. v. i., Brno, Czech Republic. Electronic address: bobalova@iach.cz.
authors.forename: J
authors.initials: J
authors.lastname: Bobalova
mesh_terms: D033942:Adaptor Proteins, Vesicular Transport / Q000378:metabolism; D000077556:Alitretinoin; D000971:Antineoplastic Combined Chemotherapy Protocols / Q000494:pharmacology*; D001943:Breast Neoplasms / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D002465:Cell Movement / Q000187:drug effects; D051338:Cofilin 1 / Q000378:metabolism; D003599:Cytoskeleton / Q000187:drug effects / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D055551:HSP27 Heat-Shock Proteins / Q000378:metabolism; D006801:Humans; D009361:Neoplasm Invasiveness; D009363:Neoplasm Proteins / Q000378:metabolism*; D040901:Proteomics / Q000379:methods; D014212:Tretinoin / Q000494:pharmacology*; D055517:snRNP Core Proteins / Q000378:metabolism
chemical_list: C570280:AP5B1 protein, human; D033942:Adaptor Proteins, Vesicular Transport; C495810:CFL1 protein, human; D051338:Cofilin 1; D055551:HSP27 Heat-Shock Proteins; D009363:Neoplasm Proteins; C526412:SNRPD3 protein, human; D055517:snRNP Core Proteins; D000077556:Alitretinoin; D014212:Tretinoin
keywords: Biomarker; Breast cancer; MCF-7; Proteomics; Retinoids