title: Enantioselective chromatography in analysis of triacylglycerols common in edible fats and oils.
abstract: Enantiomers of racemic triacylglycerol (TAG) mixtures were separated using two chiral HPLC columns with a sample recycling system and a UV detector. A closed system without sample derivatisation enabled separation and identification by using enantiopure reference compounds of eleven racemic TAGs with C12-C22 fatty acids with 0-2 double bonds. The prolonged separation time was compensated for by fewer pretreatment steps. Presence of one saturated and one unsaturated fatty acid in the asymmetric TAG favoured the separation. Enantiomeric resolution, at the same time with stronger retention of TAGs, increased with increasing fatty acid chain length in the sn-1(3) position. Triunsaturated TAGs containing oleic, linoleic or palmitoleic acids did not separate. The elution order of enantiomers was determined by chemoenzymatically synthesised enantiopure TAGs with a co-injection method. The method is applicable to many natural fats and oils of low unsaturation level assisting advanced investigation of lipid synthesis and metabolism.
authors.affiliation: Food Chemistry and Food Development, Department of Biochemistry, University of Turku, FI-20014 Turku, Finland.
authors.forename: Marika
authors.initials: M
authors.lastname: Kalpio
authors.affiliation: Food Chemistry and Food Development, Department of Biochemistry, University of Turku, FI-20014 Turku, Finland.
authors.forename: Matts
authors.initials: M
authors.lastname: Nylund
authors.affiliation: Food Chemistry and Food Development, Department of Biochemistry, University of Turku, FI-20014 Turku, Finland.
authors.forename: Kaisa M
authors.initials: KM
authors.lastname: Linderborg
authors.affiliation: Food Chemistry and Food Development, Department of Biochemistry, University of Turku, FI-20014 Turku, Finland.
authors.forename: Baoru
authors.initials: B
authors.lastname: Yang
authors.affiliation: Science Institute, University of Iceland, Dunhaga 3, 107 Reykjavik, Iceland.
authors.forename: Björn
authors.initials: B
authors.lastname: Kristinsson
authors.affiliation: Science Institute, University of Iceland, Dunhaga 3, 107 Reykjavik, Iceland.
authors.forename: Gudmundur G
authors.initials: GG
authors.lastname: Haraldsson
authors.affiliation: Food Chemistry and Food Development, Department of Biochemistry, University of Turku, FI-20014 Turku, Finland. Electronic address: heikki.kallio@utu.fi.
authors.forename: Heikki
authors.initials: H
authors.lastname: Kallio
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004041:Dietary Fats / Q000032:analysis*; D004042:Dietary Fats, Unsaturated / Q000032:analysis*; D013237:Stereoisomerism; D014280:Triglycerides / Q000032:analysis*
chemical_list: D004041:Dietary Fats; D004042:Dietary Fats, Unsaturated; D014280:Triglycerides
keywords: Chiral; Enantiomer; High-performance liquid chromatography; Stereoisomer; Triacylglycerol