title: High-performance liquid chromatographic column packings with different particle sizes: chromatographic behavior for the quality analysis of HuanglianShangqing pill.
abstract: The chromatographic separation of traditional Chinese medicines is still a highly challenging task in analytical science with respect to its hundreds and thousands of chemical compounds, while increase of separation efficiency can greatly improve the separation power of chromatographic column for traditional Chinese medicine. In this study, 13 bioactive components in HuanglianShangqing pill were selected as an index to optimize the separation conditions and evaluate the system suitability of three commercially available columns packed with 1.8, 3.5, and 5.0 μm particles. The chromatographic separations were obtained by the most appropriate Eclipse Plus C18 column (100 × 2.1 mm, 3.5 μm) within 45 min using gradient elution with aqueous-ammonium acetate (10 mmol/L, pH 5.0) and acetonitrile, at a flow rate of 0.3 mL/min and an operating temperature of 30°C. The quality of HuanglianShangqing pill was assessed through combining simultaneous quantification of 13 compounds with fingerprint analysis. For the qualitative analysis, mass spectrometry was used to confirm the 13 compounds. All the validation data conformed to the acceptable requirements. For the fingerprint analysis, 32 peaks were selected as the common peaks at 254 nm to evaluate the similarities among HuanglianShangqing pills obtained from ten manufacturers.
authors.affiliation: Jiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, Xuzhou Medical College, Xuzhou, China; Department of Pharmaceutical Analysis, Xuzhou Medical College, Xuzhou, China.
authors.forename: Dongzhi
authors.initials: D
authors.lastname: Yang
authors.forename: Ying
authors.initials: Y
authors.lastname: Li
authors.forename: Fangxiu
authors.initials: F
authors.lastname: Yang
authors.forename: Yan
authors.initials: Y
authors.lastname: Du
authors.forename: Yinjie
authors.initials: Y
authors.lastname: Li
authors.forename: Xiaoxiao
authors.initials: X
authors.lastname: Zheng
authors.forename: Daoquan
authors.initials: D
authors.lastname: Tang
mesh_terms: D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000032:analysis*; D008516:Medicine, Chinese Traditional; D010316:Particle Size; D011786:Quality Control
chemical_list: D004365:Drugs, Chinese Herbal
keywords: High-performance liquid chromatography; HuanglianShangqing pill; Quality control; Separation efficiency