title: A simple and rapid method to identify and quantitatively analyze triterpenoid saponins in Ardisia crenata using ultrafast liquid chromatography coupled with electrospray ionization quadrupole mass spectrometry.
abstract: Ardisia plant species have been used in traditional medicines, and their bioactive constituents of 13,28-epoxy triterpenoid saponins have excellent biological activities for new drug development. In this study, a fast and simple method based on ultrafast liquid chromatography coupled to electrospray ionization mass spectrometry (UFLC-MS) was developed to simultaneously identify and quantitatively analyze triterpenoid saponins in Ardisia crenata extracts. In total, 22 triterpenoid saponins, including two new compounds, were identified from A. crenata. The method exhibited good linearity, precision and recovery for the quantitative analysis of eight marker saponins. A relative quantitative method was also developed using one major saponin (ardisiacrispin B) as the standard to break through the choke-point of the lack of standards in phytochemical analysis. The method was successfully applied to quantitatively analyze saponins in commercially available plant samples. This study describes the first systematic analysis of 13,28-epoxy-oleanane-type triterpenoid saponins in the genus Ardisia using LC-ESI-MS. The results can provide the chemical support for further biological studies, phytochemotaxonomical studies and quality control of triterpenoid saponins in medicinal plants of the genus Ardisia.
authors.affiliation: Faculty of Pharmaceutical Sciences, Toho University, Miyama 2-2-1, Funabashi, Chiba 274-8510, Japan; Ningxia Institute for Drug Control, Yinchuan 750004, People's Republic of China.
authors.forename: Ling
authors.initials: L
authors.lastname: Ma
authors.affiliation: Faculty of Pharmaceutical Sciences, Toho University, Miyama 2-2-1, Funabashi, Chiba 274-8510, Japan. Electronic address: liwei@phar.toho-u.ac.jp.
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.affiliation: College of Pharmacy, Ningxia Medical University, Yinchuan 750004, People's Republic of China.
authors.forename: Hanqing
authors.initials: H
authors.lastname: Wang
authors.affiliation: Faculty of Pharmaceutical Sciences, Toho University, Miyama 2-2-1, Funabashi, Chiba 274-8510, Japan.
authors.forename: Xinzhu
authors.initials: X
authors.lastname: Kuang
authors.affiliation: Laboratory Center, The Fourth Affiliated Hospital, China Medical University, Shenyang 110032, People's Republic of China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Li
authors.affiliation: Ningxia Institute for Drug Control, Yinchuan 750004, People's Republic of China.
authors.forename: Yinghua
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Ningxia Institute for Drug Control, Yinchuan 750004, People's Republic of China.
authors.forename: Peng
authors.initials: P
authors.lastname: Xie
authors.affiliation: Faculty of Pharmaceutical Sciences, Toho University, Miyama 2-2-1, Funabashi, Chiba 274-8510, Japan.
authors.forename: Kazuo
authors.initials: K
authors.lastname: Koike
mesh_terms: D031644:Ardisia; D002853:Chromatography, Liquid / Q000379:methods; D010936:Plant Extracts / Q000032:analysis* / Q000737:chemistry; D018517:Plant Roots; D012503:Saponins / Q000032:analysis* / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods*; D013997:Time Factors; D014315:Triterpenes / Q000032:analysis* / Q000737:chemistry
chemical_list: D010936:Plant Extracts; D012503:Saponins; D014315:Triterpenes
keywords: Ardisia crenata; Identification; LC–MS; Quantitative determination; Saponin