title: Unusual association of NDM-1 with KPC-2 and armA among Brazilian Enterobacteriaceae isolates.
abstract: We report the microbiological characterization of four New Delhi metallo-β-lactamase-1 (blaNDM-1)-producing Enterobacteriaceae isolated in Rio de Janeiro, Brazil. blaNDM-1 was located on a conjugative plasmid and was associated with Klebsiella pneumoniae carbapenemase-2 (blaKPC-2) or aminoglycoside-resistance methylase (armA), a 16S rRNA methylase not previously reported in Brazil, in two distinct strains of Enterobacter cloacae. Our results suggested that the introduction of blaNDM-1 in Brazil has been accompanied by rapid spread, since our isolates showed no genetic relationship.
authors.affiliation: Laboratório Especial de Microbiologia Clínica, Departamento de Medicina, Universidade Federal de São Paulo, São Paulo, SP, Brasil.
authors.forename: M G
authors.initials: MG
authors.lastname: Quiles
authors.affiliation: Laboratório Especial de Microbiologia Clínica, Departamento de Medicina, Universidade Federal de São Paulo, São Paulo, SP, Brasil.
authors.forename: T T
authors.initials: TT
authors.lastname: Rocchetti
authors.affiliation: Laboratório Especial de Microbiologia Clínica, Departamento de Medicina, Universidade Federal de São Paulo, São Paulo, SP, Brasil.
authors.forename: L C
authors.initials: LC
authors.lastname: Fehlberg
authors.affiliation: Laboratório Diagnósticos da América, DASA, São Paulo, SP, Brasil.
authors.forename: E J U
authors.initials: EJ
authors.lastname: Kusano
authors.affiliation: Laboratório Diagnósticos da América, DASA, São Paulo, SP, Brasil.
authors.forename: A
authors.initials: A
authors.lastname: Chebabo
authors.affiliation: Laboratório Diagnósticos da América, DASA, São Paulo, SP, Brasil.
authors.forename: R M G
authors.initials: RM
authors.lastname: Pereira
authors.affiliation: Laboratório Especial de Microbiologia Clínica, Departamento de Medicina, Universidade Federal de São Paulo, São Paulo, SP, Brasil.
authors.forename: A C
authors.initials: AC
authors.lastname: Gales
authors.affiliation: Laboratório Especial de Microbiologia Clínica, Departamento de Medicina, Universidade Federal de São Paulo, São Paulo, SP, Brasil.
authors.forename: A C C
authors.initials: AC
authors.lastname: Pignatari
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000235:genetics*; D001938:Brazil; D015780:Carbapenems / Q000494:pharmacology; D003227:Conjugation, Genetic; D052978:Disk Diffusion Antimicrobial Tests; D004755:Enterobacteriaceae / Q000187:drug effects / Q000302:isolation & purification / Q000378:metabolism*; D008780:Methyltransferases / Q000235:genetics*; D008826:Microbial Sensitivity Tests; D018440:beta-Lactam Resistance / Q000235:genetics*; D001618:beta-Lactamases / Q000235:genetics*
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D015780:Carbapenems; D008780:Methyltransferases; C047346:16S rRNA (adenine(1518)-N(6)-adenine(1519)-N(6))-dimethyltransferase; D001618:beta-Lactamases; C552864:beta-lactamase NDM-1; C531581:carbapenemase-2, Klebsiella pneumoniae