title: Exposure to zearalenone mycotoxin alters in vitro porcine intestinal epithelial cells by differential gene expression.
abstract: The gut represents the main route of intoxication with mycotoxins. To evaluate the effect and the underlying molecular changes that occurred when the intestine is exposed to zearalenone, a Fusarium sp mycotoxin, porcine epithelial cells (IPEC-1) were treated with 10μM of ZEA for 24h and analysed by microarray using Gene Spring GX v.11.5. Our results showed that 10μM of ZEA did not affect cell viability, but can increase the expression of toll like receptors (TLR1-10) and of certain cytokines involved in inflammation (TNF-α, IL-1β, IL-6, IL-8, MCP-1, IL-12p40, CCL20) or responsible for the recruitment of immune cells (IL-10, IL-18). Microarray results identified 190 genes significantly and differentially expressed, of which 70% were up-regulated. ZEA determined the over expression of ITGB5 gene, essential against the attachment and adhesion of ETEC to porcine jejunal cells and of TFF2 implicated in mucosal protection. An up-regulation of glutathione peroxidase enzymes (GPx6, GPx2, GPx1) was also observed. Upon ZEA challenge, genes like GTF3C4 responsible for the recruitment of polymerase III and initiation of tRNA transcription in eukaryotes and STAT5B were significantly higher induced. The up-regulation of CD97 gene and the down-regulation of tumour suppressor genes (DKK-1, PCDH11X and TC531386) demonstrates the carcinogenic potential of ZEA.
authors.affiliation: Laboratory of Animal Biology, National Institute for Research and Development for Biology and Animal Nutrition, Calea Bucuresti No. 1, Balotesti, Ilfov 077015, Romania. Electronic address: ionelia.taranu@ibna.ro.
authors.forename: Ionelia
authors.initials: I
authors.lastname: Taranu
authors.affiliation: National Institute for Research and Development for Oncology "Prof. Dr. Ion Chiricuta", Str. Republicii, No. 34-36, Cluj-Napoca, Romania.
authors.forename: Cornelia
authors.initials: C
authors.lastname: Braicu
authors.affiliation: Laboratory of Animal Biology, National Institute for Research and Development for Biology and Animal Nutrition, Calea Bucuresti No. 1, Balotesti, Ilfov 077015, Romania.
authors.forename: Daniela Eliza
authors.initials: DE
authors.lastname: Marin
authors.affiliation: Laboratory of Animal Biology, National Institute for Research and Development for Biology and Animal Nutrition, Calea Bucuresti No. 1, Balotesti, Ilfov 077015, Romania.
authors.forename: Gina Cecilia
authors.initials: GC
authors.lastname: Pistol
authors.affiliation: Laboratory of Animal Biology, National Institute for Research and Development for Biology and Animal Nutrition, Calea Bucuresti No. 1, Balotesti, Ilfov 077015, Romania.
authors.forename: Monica
authors.initials: M
authors.lastname: Motiu
authors.affiliation: National Institute for Research and Development for Oncology "Prof. Dr. Ion Chiricuta", Str. Republicii, No. 34-36, Cluj-Napoca, Romania.
authors.forename: Loredana
authors.initials: L
authors.lastname: Balacescu
authors.affiliation: National Institute for Research and Development for Oncology "Prof. Dr. Ion Chiricuta", Str. Republicii, No. 34-36, Cluj-Napoca, Romania.
authors.forename: Ioana
authors.initials: I
authors.lastname: Beridan Neagoe
authors.affiliation: Mathematics and Physics Department, University of Agriculture and Veterinary Medicine, Bulevardul Marasti No. 59, Bucharest 011464, Romania.
authors.forename: Radu
authors.initials: R
authors.lastname: Burlacu
mesh_terms: D000818:Animals; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004847:Epithelial Cells / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D020869:Gene Expression Profiling / Q000379:methods; D005786:Gene Expression Regulation / Q000187:drug effects*; D020128:Inhibitory Concentration 50; D007413:Intestinal Mucosa / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D020411:Oligonucleotide Array Sequence Analysis; D060888:Real-Time Polymerase Chain Reaction; D013552:Swine; D013997:Time Factors; D015025:Zearalenone / Q000633:toxicity*
chemical_list: D015025:Zearalenone
keywords: Epithelial intestinal cell (IPEC-1); Gene expression; Microarray; Pig; Zearalenone