title: Chemiluminescent imaging of transpired ethanol from the palm for evaluation of alcohol metabolism.
abstract: A 2-dimensional imaging system was constructed and applied in measurements of gaseous ethanol emissions from the human palm. This imaging system measures gaseous ethanol concentrations as intensities of chemiluminescence by luminol reaction induced by alcohol oxidase and luminol-hydrogen peroxide-horseradish peroxidase system. Conversions of ethanol distributions and concentrations to 2-dimensional chemiluminescence were conducted on an enzyme-immobilized mesh substrate in a dark box, which contained a luminol solution. In order to visualize ethanol emissions from human palm skin, we developed highly sensitive and selective imaging system for transpired gaseous ethanol at sub ppm-levels. Thus, a mixture of a high-purity luminol solution of luminol sodium salt HG solution instead of standard luminol solution and an enhancer of eosin Y solution was adapted to refine the chemiluminescent intensity of the imaging system, and improved the detection limit to 3 ppm gaseous ethanol. The highly sensitive imaging allows us to successfully visualize the emissions dynamics of transdermal gaseous ethanol. The intensity of each site on the palm shows the reflection of ethanol concentrations distributions corresponding to the amount of alcohol metabolized upon consumption. This imaging system is significant and useful for the assessment of ethanol measurement of the palmar skin.
authors.affiliation: Department of Biomedical Devices and Instrumentation, Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, 2-3-10, Kanda-Surugadai, Chiyoda-ku, Tokyo 101-0062, Japan.
authors.forename: Takahiro
authors.initials: T
authors.lastname: Arakawa
authors.affiliation: Department of Biomedical Devices and Instrumentation, Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, 2-3-10, Kanda-Surugadai, Chiyoda-ku, Tokyo 101-0062, Japan.
authors.forename: Kazutaka
authors.initials: K
authors.lastname: Kita
authors.affiliation: Department of Biomedical Devices and Instrumentation, Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, 2-3-10, Kanda-Surugadai, Chiyoda-ku, Tokyo 101-0062, Japan.
authors.forename: Xin
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Biomedical Devices and Instrumentation, Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, 2-3-10, Kanda-Surugadai, Chiyoda-ku, Tokyo 101-0062, Japan.
authors.forename: Kumiko
authors.initials: K
authors.lastname: Miyajima
authors.affiliation: Department of Biomedical Devices and Instrumentation, Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, 2-3-10, Kanda-Surugadai, Chiyoda-ku, Tokyo 101-0062, Japan.
authors.forename: Koji
authors.initials: K
authors.lastname: Toma
authors.affiliation: Department of Biomedical Devices and Instrumentation, Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, 2-3-10, Kanda-Surugadai, Chiyoda-ku, Tokyo 101-0062, Japan. Electronic address: m.bdi@tmd.ac.jp.
authors.forename: Kohji
authors.initials: K
authors.lastname: Mitsubayashi
mesh_terms: D015374:Biosensing Techniques; D000431:Ethanol / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism*; D006225:Hand; D006735:Horseradish Peroxidase / Q000737:chemistry; D006801:Humans; D006861:Hydrogen Peroxide / Q000737:chemistry; D007700:Kinetics; D049449:Luminescence; D008165:Luminol / Q000737:chemistry
chemical_list: D000431:Ethanol; D008165:Luminol; D006861:Hydrogen Peroxide; D006735:Horseradish Peroxidase
keywords: Chemiluminescence; Gaseous ethanol; Imaging system; Oral alcohol administration; Transdermal emissions