title: Biological oxidation of Mn(II) coupled with nitrification for removal and recovery of minor metals by downflow hanging sponge reactor.
abstract: Biogenic manganese oxides (bio-MnO₂) have been shown to absorb minor metals. Bioreactor cultivation of heterotrophic manganese oxidizing bacteria (MnOB), which produce bio-MnO₂ via oxidation of Mn (II), can be expected to be involved in a promising system for removal and recovery of minor metals from wastewater. However, MnOB enrichment in wastewater treatment is difficult. This study investigated whether MnOB can be cultivated when coupled with nitrification in a system in which soluble microbial products (SMP) from nitrifiers are provided to MnOB as a substrate. A downflow hanging sponge (DHS) reactor was applied for MnOB cultivation with ammonium (NH₄⁺) and Mn (II) continuously supplied. During long-term operation, Mn (II) oxidation was successfully established at a rate of 48 g Mn m⁻³ d⁻¹ and bio-MnO₂ that formed on the sponges were recovered from the bottom of the reactor. The results also revealed that Ni and Co added to the influent were simultaneously removed. Microbial 16S rRNA gene clone analysis identified nitrifiers supporting MnOB growth and showed that only one clone of Bacillus subtilis, which was affiliated with a known MnOB cluster, was present, suggesting the existence of other novel bacteria with the ability to oxidize Mn (II).
authors.affiliation: Department of Civil and Environmental Engineering, Graduate School of Engineering, Hiroshima University, 1-4-1 Kagamiyama, Higashi-Hiroshima, Hiroshima 739-8527, Japan.
authors.forename: Linh Thi Thuy
authors.initials: LT
authors.lastname: Cao
authors.forename: Hiroya
authors.initials: H
authors.lastname: Kodera
authors.forename: Kenichi
authors.initials: K
authors.lastname: Abe
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Imachi
authors.forename: Yoshiteru
authors.initials: Y
authors.lastname: Aoi
authors.forename: Tomonori
authors.initials: T
authors.lastname: Kindaichi
authors.forename: Tomonori
authors.initials: T
authors.lastname: Ozaki
authors.forename: Akiyoshi
authors.initials: A
authors.lastname: Ohashi
mesh_terms: D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000378:metabolism; D018533:Biomass; D019149:Bioreactors / Q000382:microbiology*; D017895:Manganese Compounds / Q000737:chemistry / Q000378:metabolism*; D008670:Metals / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D008969:Molecular Sequence Data; D058465:Nitrification; D010084:Oxidation-Reduction; D010087:Oxides / Q000737:chemistry / Q000378:metabolism*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D015203:Reproducibility of Results; D017422:Sequence Analysis, DNA; D014865:Waste Disposal, Fluid / Q000295:instrumentation / Q000379:methods*; D062065:Wastewater / Q000737:chemistry / Q000382:microbiology
chemical_list: D017895:Manganese Compounds; D008670:Metals; D010087:Oxides; D012336:RNA, Ribosomal, 16S; D062065:Wastewater; C016552:manganese dioxide