title: Regulation of the subcellular shuttling of Sgo1 between centromeres and chromosome arms by Aurora B-mediated phosphorylation.
abstract: A minor fraction of cohesin complexes at chromosome arms is not removed by the prophase pathway, and maintained until metaphase and enriched at centromeres. Sgo1 localizes to chromosome arms from prophase to metaphase, and is indispensable for removing cohesin complexes from chromosome arms. However, it has not been established how the chromosome arm localization of Sgo1 leads to the establishment of cohesion on chromosomes. Here, we report that Aurora B kinase interacts with and phosphorylates Sgo1 in vitro and in vivo. Furthermore, the phosphorylation of Sgol by Aurora B kinase regulated the distribution of Sgo1 between centromeres and chromosome arms, and the expression of Aurora B kinase-dead mutants of Sgo1 caused mislocalization from centromeres to chromosome arms. These results suggest Aurora B kinase directly regulates the subcellular distribution of Sgo1 to facilitate the accurate separation of mitotic chromosomes.
authors.affiliation: Department of Molecular Cell Biology, Samsung Biomedical Research Institute, Sungkyunkwan University School of Medicine, Suwon 440-746, Republic of Korea.
authors.forename: Noo Ri
authors.initials: NR
authors.lastname: Lee
authors.affiliation: Department of Molecular Cell Biology, Samsung Biomedical Research Institute, Sungkyunkwan University School of Medicine, Suwon 440-746, Republic of Korea. Electronic address: jazz7780@skku.edu.
authors.forename: Hyun-Soo
authors.initials: HS
authors.lastname: Kim
authors.affiliation: Department of Biochemistry, Ajou University School of Medicine, Suwon 443-749, Republic of Korea.
authors.forename: You-Sun
authors.initials: YS
authors.lastname: Kim
authors.affiliation: Department of Microbiology, Ajou University School of Medicine, Suwon 443-749, Republic of Korea.
authors.forename: Myung-Hee
authors.initials: MH
authors.lastname: Kwon
authors.affiliation: Department of Biochemistry and Biomedical Sciences, Ajou University School of Medicine, Suwon 443-749, Republic of Korea.
authors.forename: Kyeong Sook
authors.initials: KS
authors.lastname: Choi
authors.affiliation: Department of Molecular Cell Biology, Samsung Biomedical Research Institute, Sungkyunkwan University School of Medicine, Suwon 440-746, Republic of Korea. Electronic address: cwlee1234@skku.edu.
authors.forename: Chang-Woo
authors.initials: CW
authors.lastname: Lee
mesh_terms: D064107:Aurora Kinase B / Q000032:analysis / Q000378:metabolism*; D018797:Cell Cycle Proteins / Q000032:analysis / Q000378:metabolism*; D002503:Centromere / Q000378:metabolism* / Q000648:ultrastructure; D020090:Chromosome Segregation; D006367:HeLa Cells; D006801:Humans; D008938:Mitosis; D010766:Phosphorylation
chemical_list: D018797:Cell Cycle Proteins; C496644:SGO1 protein, human; D064107:Aurora Kinase B
keywords: Aurora B; Centromere; Chromosome arm; Phosphorylation; Sgo1; Sister chromatid cohesion