title: Generalized polymer effective charge measurement by capillary isotachophoresis.
abstract: In this work, we have generalized the use of capillary isotachophoresis as a universal method for determination of effective charge of anionic and cationic (co)polymers on ordinary capillary electrophoresis instruments. This method is applicable to a broad range of strong or weak polyelectrolytes with good repeatability. Experimental parameters (components and concentrations of leading and terminating electrolytes, capillary diameters, constant electric current intensity) were optimized for implementation in 100 μm i.d. capillaries for both polyanions and polycations. Determined values of polymer effective charge were in a very good agreement with those obtained by capillary electrophoresis with indirect UV detection. Uncertainty of the effective charge measurement using isotachophoresis was addressed and estimated to be ∼5-10% for solutes with mobilities in the 20-50 × 10(-9)m(2)V(-1)s(-1) range.
authors.affiliation: Institut des Biomolécules Max Mousseron (UMR 5247 CNRS-Université de Montpellier 1-Université de Montpellier 2), place Eugène Bataillon CC 1706, 34095 Montpellier Cedex 5, France.
authors.forename: Joseph
authors.initials: J
authors.lastname: Chamieh
authors.affiliation: Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, v.v.i., Flemingovo n. 2, 166 10 Prague 6, Czech Republic.
authors.forename: Dušan
authors.initials: D
authors.lastname: Koval
authors.affiliation: Institut des Biomolécules Max Mousseron (UMR 5247 CNRS-Université de Montpellier 1-Université de Montpellier 2), place Eugène Bataillon CC 1706, 34095 Montpellier Cedex 5, France.
authors.forename: Adeline
authors.initials: A
authors.lastname: Besson
authors.affiliation: Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, v.v.i., Flemingovo n. 2, 166 10 Prague 6, Czech Republic.
authors.forename: Václav
authors.initials: V
authors.lastname: Kašička
authors.affiliation: Institut des Biomolécules Max Mousseron (UMR 5247 CNRS-Université de Montpellier 1-Université de Montpellier 2), place Eugène Bataillon CC 1706, 34095 Montpellier Cedex 5, France. Electronic address: hcottet@univ-montp2.fr.
authors.forename: Hervé
authors.initials: H
authors.lastname: Cottet
mesh_terms: D000838:Anions / Q000737:chemistry; D002412:Cations / Q000737:chemistry; D004573:Electrolytes; D019075:Electrophoresis, Capillary / Q000295:instrumentation / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D057625:Isotachophoresis / Q000295:instrumentation / Q000379:methods*; D011108:Polymers / Q000737:chemistry*; D015203:Reproducibility of Results
chemical_list: D000838:Anions; D002412:Cations; D004573:Electrolytes; D011108:Polymers
keywords: Capillary electrophoresis; Counter-ion condensation; Isotachophoresis; Polyelectrolyte; Polymer effective charge