title: Optimization of culture parameters and novel strategies to improve protein solubility.
abstract: The production of recombinant proteins, in soluble form in a prokaryotic expression system, still remains a challenge for the biotechnologist. Innovative strategies have been developed to improve protein solubility in various protein overexpressing hosts. In this chapter, we would focus on methods currently available and amenable to "desired modifications," such as (a) the use of molecular chaperones; (b) the optimization of culture conditions; (c) the reengineering of a variety of host strains and vectors with affinity tags; and (d) optimal promoter strengths. All these parameters are evaluated with the primary objective of increasing the solubilization of recombinant protein(s) during overexpression in Escherichia coli.
authors.affiliation: School of Biotechnology, Jawaharlal Nehru University, New Delhi, 110067, India.
authors.forename: Ranjana
authors.initials: R
authors.lastname: Arya
authors.forename: Jamal S M
authors.initials: JS
authors.lastname: Sabir
authors.forename: Roop S
authors.initials: RS
authors.lastname: Bora
authors.forename: Kulvinder S
authors.initials: KS
authors.lastname: Saini
mesh_terms: D000818:Animals; D001709:Biotechnology / Q000379:methods; D003470:Culture Media / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D005822:Genetic Vectors / Q000235:genetics / Q000378:metabolism; D006801:Humans; D018832:Molecular Chaperones / Q000235:genetics / Q000378:metabolism; D011401:Promoter Regions, Genetic / Q000235:genetics; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism*; D012995:Solubility
chemical_list: D003470:Culture Media; D018832:Molecular Chaperones; D011994:Recombinant Proteins