title: Antiplatelet aggregation triterpene saponins from the barks of Ilex rotunda.
abstract: Four new triterpene saponins, rotundinosides A-D (1-4) and seven known triterpene saponins (5-11) were isolated from a methanol extract of the barks of Ilex rotunda Thunb. The new saponins were characterized as 3-O-β-d-glucopyranosy1-(1→2)-β-d-xylopyranosyl siaresinolic acid 28-O-β-d-glucopyranoside (1), 3-O-[β-d-glucopyranosy1-(1→2)-β-d-xylopyranosyl]-3β,19α-dihydroxyurs-12-en-28-oic-O-β-d-glucopranosy1ester (2), 3-O-[α-l-rhamnopyranosyl-(1→2)-β-d-glucopyranosy1-(1→2)-α-l-arabinopyranosyl]-3β,19α-dihydroxyurs-12-en-28-oic-O-β-d-glucopyranosy1 ester (3), and 3-O-α-l-rhamanopyranosyl-(1→2)-β-d-glucopyranosy1-(1→2)-α-l-arabinopyranosyl ilexgenin B 28-O-β-d-glucopyranosy1 ester (4), respectively. Their structures were established by extensive spectroscopic analysis, including HSQC, HMBC, (1)H-(1)H COSY, NOESY and acid hydrolysis, and also by the comparison of their spectroscopic data with those of related compounds. The known compounds 5-11 were all obtained from this species for the first time. The biological activity of compounds 1-11 against ADP induced platelet aggregation in rabbit plasma was determined. Among the tested compounds 1, 3, 5 and 10 exhibited strong inhibition of platelet aggregation in vitro, with IC50 values of 11.4±2.2, 10.4±1.3, 13.2±2.4, and 15.1±3.4μM, respectively.
authors.affiliation: School of Chinese Materia Medica, Guangzhou University of Chinese Medicine, Guangzhou, 510006, China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Fan
authors.affiliation: School of Chinese Materia Medica, Guangzhou University of Chinese Medicine, Guangzhou, 510006, China.
authors.forename: Lian
authors.initials: L
authors.lastname: Zhou
authors.affiliation: School of Chinese Materia Medica, Guangzhou University of Chinese Medicine, Guangzhou, 510006, China.
authors.forename: Tianqin
authors.initials: T
authors.lastname: Xiong
authors.affiliation: School of Chinese Materia Medica, Guangzhou University of Chinese Medicine, Guangzhou, 510006, China.
authors.forename: Jinsong
authors.initials: J
authors.lastname: Zhou
authors.affiliation: School of Chinese Materia Medica, Guangzhou University of Chinese Medicine, Guangzhou, 510006, China.
authors.forename: Qingguo
authors.initials: Q
authors.lastname: Li
authors.affiliation: School of Chinese Materia Medica, Guangzhou University of Chinese Medicine, Guangzhou, 510006, China.
authors.forename: Qinglong
authors.initials: Q
authors.lastname: Tan
authors.affiliation: School of Chinese Materia Medica, Guangzhou University of Chinese Medicine, Guangzhou, 510006, China. Electronic address: zzx37@163.com.
authors.forename: Zhongxiang
authors.initials: Z
authors.lastname: Zhao
authors.affiliation: School of Pharmaceutical Sciences, Sun Yat-Sen University, Guangzhou 510006, China. Electronic address: jinjing@mail.sysu.edu.cn.
authors.forename: Jing
authors.initials: J
authors.lastname: Jin
mesh_terms: D000818:Animals; D001792:Blood Platelets / Q000187:drug effects*; D030017:Ilex / Q000737:chemistry*; D015394:Molecular Structure; D024301:Plant Bark / Q000737:chemistry; D010974:Platelet Aggregation / Q000187:drug effects*; D011817:Rabbits; D012503:Saponins / Q000737:chemistry* / Q000302:isolation & purification; D014315:Triterpenes / Q000737:chemistry* / Q000302:isolation & purification
chemical_list: D012503:Saponins; D014315:Triterpenes
keywords: Antiplatelet aggregation; Aquifoliaceae; Ilex rotunda; Rotundinosides A–D; Triterpene saponin