title: Selenium deficiency sensitizes the skin for UVB-induced oxidative damage and inflammation which involved the activation of p38 MAPK signaling.
abstract: Ultraviolet B (UVB) radiation causes oxidative damage and inflammation, and ultimately increases the risk of skin carcinogenesis. Selenium is an essential trace element, previous studies indicated selenium deficiency impairs tissue antioxidant capacity in different experimental models. However, the synergistic effect of selenium deficiency and UVB radiation on skin damage is not clear. In the current study, our data revealed selenium deficiency resulted in further increases of reactive oxygen species (ROS), thiobarbituric acid reactive substances (TBARS) and phosphorylated H2AX levels, decreases of GSH level and antioxidant enzyme activities in UVB-irradiated mice. Selenium deficiency also exacerbated UVB-induced cyclooxygenase-2 (COX-2), inducible nitric oxide synthase (iNOS), tumor necrosis factor-alpha (TNF-α), interleukin-1beta (IL-1β) and IL-6 mRNA expressions. Mechanism studies indicated that UVB-induced p38 signaling was further elevated in the skin of mice maintained with selenium deficiency diet, compared with those maintained with selenium adequate diet. Our investigation suggested that selenium deficiency diet weakens the antioxidant capacity of UVB-irradiated mice skin, which sensitizes to UV radiation-induced oxidative damage and inflammation.
authors.affiliation: Key Laboratory of Luminescence and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, College of Pharmaceutical Sciences, Southwest University, Chongqing 400715, China.
authors.forename: Xiaokang
authors.initials: X
authors.lastname: Zhu
authors.affiliation: Department of Radiation Oncology, The Ninth People's Hospital of Chongqing, Chongqing 400700, China. Electronic address: jmdxgh@163.com.
authors.forename: Mingdong
authors.initials: M
authors.lastname: Jiang
authors.affiliation: Key Laboratory of Luminescence and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, College of Pharmaceutical Sciences, Southwest University, Chongqing 400715, China.
authors.forename: Erqun
authors.initials: E
authors.lastname: Song
authors.affiliation: Department of Rehabilitation and Physiotherapy, No. 324 Hospital of PLA, Chongqing 400020, China.
authors.forename: Xiaoji
authors.initials: X
authors.lastname: Jiang
authors.affiliation: Key Laboratory of Luminescence and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, College of Pharmaceutical Sciences, Southwest University, Chongqing 400715, China. Electronic address: ysong@swu.edu.cn.
authors.forename: Yang
authors.initials: Y
authors.lastname: Song
mesh_terms: D000818:Animals; D051546:Cyclooxygenase 2 / Q000235:genetics / Q000378:metabolism; D005260:Female; D005978:Glutathione / Q000378:metabolism; D006657:Histones / Q000235:genetics / Q000378:metabolism; D007249:Inflammation / Q000473:pathology*; D053583:Interleukin-1beta / Q000235:genetics / Q000378:metabolism; D015850:Interleukin-6 / Q000235:genetics / Q000378:metabolism; D015227:Lipid Peroxidation; D020935:MAP Kinase Signaling System; D051379:Mice; D008807:Mice, Inbred BALB C; D052247:Nitric Oxide Synthase Type II / Q000235:genetics / Q000378:metabolism; D018384:Oxidative Stress / Q000528:radiation effects*; D010766:Phosphorylation; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D012643:Selenium / Q000008:administration & dosage / Q000172:deficiency*; D012867:Skin / Q000187:drug effects / Q000528:radiation effects*; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000378:metabolism; D014466:Ultraviolet Rays / Q000009:adverse effects*; D048051:p38 Mitogen-Activated Protein Kinases / Q000235:genetics / Q000378:metabolism
chemical_list: D051546:Cyclooxygenase 2; D005978:Glutathione; D006657:Histones; D053583:Interleukin-1beta; D015850:Interleukin-6; D052247:Nitric Oxide Synthase Type II; D012333:RNA, Messenger; D017382:Reactive Oxygen Species; D012643:Selenium; D017392:Thiobarbituric Acid Reactive Substances; D014409:Tumor Necrosis Factor-alpha; D048051:p38 Mitogen-Activated Protein Kinases; C496317:Nos2 protein, mouse; C077623:Ptgs2 protein, mouse; C475961:gamma-H2AX protein, mouse
keywords: Inflammation; Oxidative damage; Selenium deficiency; Skin; UV radiation; p38