title: Expression of Sox2 in breast cancer cells promotes the recruitment of M2 macrophages to tumor microenvironment.
abstract: Transcriptional factor Sox2 promotes tumor metastasis; however its regulatory effect on tumor-associated macrophages (TAMs, M2 phenotype) has not been defined. This study disclosed concomitant expression of TAMs marker-CD163 with SOX2 in human breast cancer and showed that Sox2 in breast cancer cells promotes recruitment of TAMs with altered expression of multiple chemokines, including MIP-1α, ICAM-1 etc. and activation of Stat3 and NF-κB signalings. In addition, TAMs rescued the compromised lung metastasis induced by Sox2 silencing in breast cancer cells. Together, this study documented that Sox2 plays an important role in recruiting TAMs and promotes tumor metastasis in a TAMs dependent manner.
authors.affiliation: School of Medicine, Nankai University, 94 Weijin Road, Tianjin 300071, China; Beijing Pediatric Research Institute, Beijing Children's Hospital, Capital Medical University, Beijing 100045, China; Department of Biochemistry, Chinese PLA General Hospital, Beijing 100853, China.
authors.forename: Wenjun
authors.initials: W
authors.lastname: Mou
authors.affiliation: School of Medicine, Nankai University, 94 Weijin Road, Tianjin 300071, China.
authors.forename: Yingxi
authors.initials: Y
authors.lastname: Xu
authors.affiliation: School of Medicine, Nankai University, 94 Weijin Road, Tianjin 300071, China.
authors.forename: Yujie
authors.initials: Y
authors.lastname: Ye
authors.affiliation: School of Medicine, Nankai University, 94 Weijin Road, Tianjin 300071, China.
authors.forename: Si
authors.initials: S
authors.lastname: Chen
authors.affiliation: Beijing Health Vocational College, 94 Nanhengxijie Street, Beijing 100053, China.
authors.forename: Xuefei
authors.initials: X
authors.lastname: Li
authors.affiliation: School of Medicine, Nankai University, 94 Weijin Road, Tianjin 300071, China.
authors.forename: Kangzi
authors.initials: K
authors.lastname: Gong
authors.affiliation: School of Medicine, Nankai University, 94 Weijin Road, Tianjin 300071, China.
authors.forename: Yanhua
authors.initials: Y
authors.lastname: Liu
authors.affiliation: School of Medicine, Nankai University, 94 Weijin Road, Tianjin 300071, China.
authors.forename: Yanan
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Department of General Surgery, Chinese PLA General Hospital, Beijing 100853, China.
authors.forename: Xiru
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Biochemistry, Chinese PLA General Hospital, Beijing 100853, China.
authors.forename: Yaping
authors.initials: Y
authors.lastname: Tian
authors.affiliation: School of Medicine, Nankai University, 94 Weijin Road, Tianjin 300071, China; Tianjin Key Laboratory of Tumor Microenvironment and Neurovascular Regulation, Tianjin 300071, China; Collaborative Innovation Center for Biotherapy, Nankai University, 94 Weijin Road, Tianjin 300071, China.
authors.forename: Rong
authors.initials: R
authors.lastname: Xiang
authors.affiliation: School of Medicine, Nankai University, 94 Weijin Road, Tianjin 300071, China; Tianjin Key Laboratory of Tumor Microenvironment and Neurovascular Regulation, Tianjin 300071, China; Collaborative Innovation Center for Biotherapy, Nankai University, 94 Weijin Road, Tianjin 300071, China. Electronic address: lina08@nankai.edu.cn.
authors.forename: Na
authors.initials: N
authors.lastname: Li
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000235:genetics / Q000378:metabolism; D015214:Antigens, Differentiation, Myelomonocytic / Q000235:genetics / Q000378:metabolism; D001943:Breast Neoplasms / Q000235:genetics* / Q000276:immunology* / Q000378:metabolism / Q000473:pathology; D045744:Cell Line, Tumor; D002634:Chemotaxis, Leukocyte / Q000235:genetics / Q000276:immunology; D016207:Cytokines / Q000235:genetics / Q000378:metabolism; D004195:Disease Models, Animal; D005260:Female; D015870:Gene Expression; D015972:Gene Expression Regulation, Neoplastic; D020868:Gene Silencing; D064593:Heterografts; D006801:Humans; D007150:Immunohistochemistry; D008264:Macrophages / Q000276:immunology* / Q000378:metabolism* / Q000473:pathology; D051379:Mice; D016328:NF-kappa B / Q000378:metabolism; D050778:NFATC Transcription Factors / Q000378:metabolism; D009362:Neoplasm Metastasis; D011956:Receptors, Cell Surface / Q000235:genetics / Q000378:metabolism; D055748:SOXB1 Transcription Factors / Q000235:genetics* / Q000378:metabolism; D050796:STAT3 Transcription Factor / Q000378:metabolism; D015398:Signal Transduction; D059016:Tumor Microenvironment; D000098902:CD163 Antigen
chemical_list: D015703:Antigens, CD; D015214:Antigens, Differentiation, Myelomonocytic; D000098902:CD163 Antigen; D016207:Cytokines; D016328:NF-kappa B; D050778:NFATC Transcription Factors; D011956:Receptors, Cell Surface; D055748:SOXB1 Transcription Factors; D050796:STAT3 Transcription Factor
keywords: CD163; Sox2; Tumor microenvironment; Tumor-associated macrophages