title: Isolation, cultivation and identification of human lung adenocarcinoma stem cells.
abstract: Recently, an increasing number of studies have demonstrated that lung cancer is a stem cell disease. However, ideal cell surface markers for isolating stem cells in lung cancer are yet to be identified. In the present study, a cell population with a cluster of differentiation (CD)133+phenotype was successfully isolated from a single cell suspension of lung adenocarcinoma tissue using magnetic-activated cell sorting (MACS) and enriched in a serum-free culture. In comparison to CD133-cells, the CD133+cells exhibited an enhanced capacity for self-renewal and differentiation, and a greater potential forin vivotumor formation, in non-obese diabetic/severe combined immunodeficient (NOD/SCID) mice. Tumors could be induced in NOD/SCID mice by the transplantation of 102stem-like cells per mouse. The results of the present study demonstrated that CD133 may serve as a specific cell surface marker for lung adenocarcinoma stem cells, and that MACS combined with serum-free culture is an effective method for isolating and enriching lung cancer stem cells.
authors.affiliation: Department of Oncology, Taizhou People's Hospital Affiliated to Medical College of Nantong University, Taizhou, Jiangsu 225300, P.R. China.
authors.forename: DE-Geng
authors.initials: DG
authors.lastname: Zhang
authors.affiliation: Department of Respiratory Medicine, Taizhou People's Hospital Affiliated to Medical College of Nantong University, Taizhou, Jiangsu 225300, P.R. China.
authors.forename: Ai-Gui
authors.initials: AG
authors.lastname: Jiang
authors.affiliation: Department of Respiratory Medicine, Taizhou People's Hospital Affiliated to Medical College of Nantong University, Taizhou, Jiangsu 225300, P.R. China.
authors.forename: Hui-Yu
authors.initials: HY
authors.lastname: Lu
authors.affiliation: Institute of Clinical Medicine, Taizhou People's Hospital Affiliated to Medical College of Nantong University, Taizhou, Jiangsu 225300, P.R. China.
authors.forename: Li-Xin
authors.initials: LX
authors.lastname: Zhang
authors.affiliation: Department of Respiratory Medicine, Taizhou People's Hospital Affiliated to Medical College of Nantong University, Taizhou, Jiangsu 225300, P.R. China.
authors.forename: Xiao-Yan
authors.initials: XY
authors.lastname: Gao
keywords: cell separation; cluster of differentiation 133; lung cancer; lung cancer stem cell