title: Tissue specific expression of SG2NA is regulated by differential splicing, RNA editing and differential polyadenylation.
abstract: SG2NA belongs to a three member Striatin subfamily of WD-40 repeat superfamily. It has multiple protein-protein interaction domains that are involved in the assembly of supra-molecular signaling complexes. Earlier we had demonstrated that there are at least five variants of SG2NA, generated by alternative splicing. We now demonstrate that a 52kDa novel variant is generated by the editing of the transcript for the 82kDa isoform. The 52kDa protein is abundant in mouse tissues but it is barely present in immortalized cells, suggesting its role in cell differentiation. Besides splicing and editing, expression of SG2NAs in tissues is also regulated by differential polyadenylation and mRNA/protein stability. Further, the longer UTR is seen only in the brain mRNA from 1month old mouse and 8-10day old chick embryo. Like alternative splicing, differential polyadenylation of Sg2na transcripts is also conserved in evolution. Taken together, these results suggest a highly versatile and dynamic mode of regulation of SG2NA with potential implications in tissue development.
authors.affiliation: School of Life Sciences, Jawaharlal Nehru University, New Delhi 110067, India.
authors.forename: Buddhi Prakash
authors.initials: BP
authors.lastname: Jain
authors.affiliation: School of Life Sciences, Jawaharlal Nehru University, New Delhi 110067, India.
authors.forename: Pooja
authors.initials: P
authors.lastname: Chauhan
authors.affiliation: School of Life Sciences, Jawaharlal Nehru University, New Delhi 110067, India.
authors.forename: Goutam K
authors.initials: GK
authors.lastname: Tanti
authors.affiliation: The University of Texas M.D. Anderson Cancer Center, Science Park, Department of Molecular Carcinogenesis, P.O. Box 389, Smithville, TX 78957, United States.
authors.forename: Nandini
authors.initials: N
authors.lastname: Singarapu
authors.affiliation: Division of Animal Sciences, Agharkar Research Institute, Pune, India.
authors.forename: Surendra
authors.initials: S
authors.lastname: Ghaskadbi
authors.affiliation: School of Life Sciences, Jawaharlal Nehru University, New Delhi 110067, India. Electronic address: skgoswami@mail.jnu.ac.in.
authors.forename: Shyamal K
authors.initials: SK
authors.lastname: Goswami
mesh_terms: D000818:Animals; D001324:Autoantigens / Q000235:genetics* / Q000378:metabolism*; D001921:Brain / Q000196:embryology / Q000378:metabolism*; D016466:CHO Cells; D002148:Calmodulin-Binding Proteins / Q000235:genetics* / Q000378:metabolism*; D045744:Cell Line, Tumor; D002642:Chick Embryo; D003412:Cricetulus; D004622:Embryo, Mammalian / Q000378:metabolism; D008297:Male; D051379:Mice; D009928:Organ Specificity; D026723:Polyadenylation; D055550:Protein Stability; D017393:RNA Editing; D012326:RNA Splicing
chemical_list: D001324:Autoantigens; D002148:Calmodulin-Binding Proteins; C485775:Strn3 protein, mouse
keywords: Differentiation; Polyadenylation; SG2NA; Striatin; WD-40 repeat; mRNA editing