title: Influence of the crosslinker type on the chromatographic properties of hydrophilic sulfoalkylbetaine-type monolithic columns.
abstract: In order to investigate the effects of the crosslinker on the separation performance of polar zwitterionic sulfoalkylbetaine-type monolithic columns, three crosslinkers, i.e. 1,4-bis(acryloyl)piperazine (PDA), ethylene dimethacrylate (EDMA) and N,N'-methylenebisacrylamide (MBA), were copolymerized with the hydrophilic monomer N,N-dimethyl-N-acryloyloxyethyl-N-(3-sulfopropyl)ammonium betaine (SPDA). The chromatographic properties of the three hydrophilic sulfoalkylbetaine-type monolithic columns, including column efficiency, permeability, porosity and separation mechanism, were systematically compared using scanning electron microscopy or micro-HPLC. Good selectivity in micro-HPLC separations was achieved on all three monolithic columns. The results indicate that the polarity of sulfoalkylbetaine-type monolithic columns may be related to the polarity of the crosslinker, which further affects column selectivity and efficiency. A particularly high column efficiency (100,000 plates/m) was obtained on the novel poly(SPDA-co-PDA) monolithic column at a linear velocity of 1mm/s using thiourea as test analyte. A higher resolution was also observed for nucleobases, nucleosides and hydrophilic organic acids on this novel poly(SPDA-co-PDA) monolithic column compared to the other two columns.
authors.affiliation: Department of Pharmacy and Guangdong Province Key Laboratory of Pharmacodynamics of Traditional Chinese Medicine & New Drug Research, Jinan University, Guangzhou 510632, China.
authors.forename: Chusheng
authors.initials: C
authors.lastname: Liu
authors.affiliation: Department of Pharmacy and Guangdong Province Key Laboratory of Pharmacodynamics of Traditional Chinese Medicine & New Drug Research, Jinan University, Guangzhou 510632, China.
authors.forename: Weijia
authors.initials: W
authors.lastname: Chen
authors.affiliation: Department of Pharmacy and Guangdong Province Key Laboratory of Pharmacodynamics of Traditional Chinese Medicine & New Drug Research, Jinan University, Guangzhou 510632, China.
authors.forename: Guangxin
authors.initials: G
authors.lastname: Yuan
authors.affiliation: Department of Pharmacy and Guangdong Province Key Laboratory of Pharmacodynamics of Traditional Chinese Medicine & New Drug Research, Jinan University, Guangzhou 510632, China.
authors.forename: Yao
authors.initials: Y
authors.lastname: Xiao
authors.affiliation: Department of Pharmacy and Guangdong Province Key Laboratory of Pharmacodynamics of Traditional Chinese Medicine & New Drug Research, Jinan University, Guangzhou 510632, China; Laboratory of Analytical Pharmaceutical Chemistry, Department of Pharmaceutical Sciences, University of Liege, CHU B36, B-4000 Liege, Belgium.
authors.forename: Jacques
authors.initials: J
authors.lastname: Crommen
authors.affiliation: Department of Chemistry, Jinan University, Guangzhou 510632, China. Electronic address: txush@jnu.edu.cn.
authors.forename: Shihai
authors.initials: S
authors.lastname: Xu
authors.affiliation: Department of Pharmacy and Guangdong Province Key Laboratory of Pharmacodynamics of Traditional Chinese Medicine & New Drug Research, Jinan University, Guangzhou 510632, China. Electronic address: jzjjackson@hotmail.com.
authors.forename: Zhengjin
authors.initials: Z
authors.lastname: Jiang
mesh_terms: D000178:Acrylamides / Q000737:chemistry; D001622:Betaine / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D003432:Cross-Linking Reagents / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D057927:Hydrophobic and Hydrophilic Interactions; D008689:Methacrylates / Q000737:chemistry; D009705:Nucleosides / Q000032:analysis; D010539:Permeability; D058105:Polymerization; D016062:Porosity; D055672:Static Electricity
chemical_list: D000178:Acrylamides; D003432:Cross-Linking Reagents; D008689:Methacrylates; D009705:Nucleosides; D001622:Betaine; C004919:ethylene dimethacrylate; C021221:N,N'-methylenebisacrylamide
keywords: Crosslinker; Hydrophilic interaction chromatography; N,N-dimethyl-N-acryloyloxyethyl-N-(3-sulfopropyl)ammonium betaine; Sulfoalkylbetaine; Zwitterionic monolithic column