title: Effect of CuO nanoparticles on the production and composition of extracellular polymeric substances and physicochemical stability of activated sludge flocs.
abstract: The effects of CuO nanoparticles (NPs) on the production and composition of extracellular polymeric substances (EPS) and the physicochemical stability of activated sludge were investigated. The results showed enhanced production of loosely bound extracellular polymeric substances (LB-EPS), protecting against nanotoxicity. Specifically, polysaccharide production increased by 89.7% compared to control upon exposure to CuO NPs (50mg/L). Fourier transform-infrared spectroscopy analysis revealed changes in the polysaccharide COC group and the carboxyl group of proteins in the EPS in the presence of CuO NPs. The sludge flocs were unstable after exposure to CuO NPs (50mg/L) because of excess LB-EPS. This also corresponded with decreased cell viability of the sludge flocs, as determined by the production of reactive oxygen species and the release of lactate dehydrogenase. These results are key to assessing the adverse effects of the CuO NPs on activated sludge in wastewater treatment plants.
authors.affiliation: Key Laboratory of Integrated Regulation and Resources Development on Shallow Lakes, Ministry of Education, Hohai University, Nanjing 210098, People's Republic of China; College of Environment, Hohai University, Nanjing 210098, People's Republic of China.
authors.forename: Jun
authors.initials: J
authors.lastname: Hou
authors.affiliation: Key Laboratory of Integrated Regulation and Resources Development on Shallow Lakes, Ministry of Education, Hohai University, Nanjing 210098, People's Republic of China; College of Environment, Hohai University, Nanjing 210098, People's Republic of China. Electronic address: mlz1988@126.com.
authors.forename: Lingzhan
authors.initials: L
authors.lastname: Miao
authors.affiliation: Key Laboratory of Integrated Regulation and Resources Development on Shallow Lakes, Ministry of Education, Hohai University, Nanjing 210098, People's Republic of China; College of Environment, Hohai University, Nanjing 210098, People's Republic of China.
authors.forename: Chao
authors.initials: C
authors.lastname: Wang
authors.affiliation: Key Laboratory of Integrated Regulation and Resources Development on Shallow Lakes, Ministry of Education, Hohai University, Nanjing 210098, People's Republic of China; College of Environment, Hohai University, Nanjing 210098, People's Republic of China.
authors.forename: Peifang
authors.initials: P
authors.lastname: Wang
authors.affiliation: Key Laboratory of Integrated Regulation and Resources Development on Shallow Lakes, Ministry of Education, Hohai University, Nanjing 210098, People's Republic of China; College of Environment, Hohai University, Nanjing 210098, People's Republic of China.
authors.forename: Yanhui
authors.initials: Y
authors.lastname: Ao
authors.affiliation: Key Laboratory of Integrated Regulation and Resources Development on Shallow Lakes, Ministry of Education, Hohai University, Nanjing 210098, People's Republic of China; College of Environment, Hohai University, Nanjing 210098, People's Republic of China.
authors.forename: Bowen
authors.initials: B
authors.lastname: Lv
mesh_terms: D065567:Absorption, Physiological; D000704:Analysis of Variance; D002681:China; D003300:Copper / Q000009:adverse effects* / Q000737:chemistry; D005427:Flocculation / Q000187:drug effects; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D053768:Metal Nanoparticles / Q000009:adverse effects* / Q000737:chemistry; D008855:Microscopy, Electron, Scanning; D011135:Polysaccharides, Bacterial / Q000096:biosynthesis*; D017382:Reactive Oxygen Species / Q000378:metabolism; D012722:Sewage / Q000737:chemistry* / Q000382:microbiology; D017550:Spectroscopy, Fourier Transform Infrared; D014865:Waste Disposal, Fluid / Q000379:methods*; D014874:Water Pollutants, Chemical / Q000009:adverse effects* / Q000737:chemistry
chemical_list: D011135:Polysaccharides, Bacterial; D017382:Reactive Oxygen Species; D012722:Sewage; D014874:Water Pollutants, Chemical; D003300:Copper; D007770:L-Lactate Dehydrogenase; C030973:cupric oxide
keywords: Activated sludge floc; Bioflocculation; Dewaterability; Extracellular polymeric substances (EPS); Nano-CuO