title: Transcriptional profile of HIV-induced nuclear translocation of amyloid β in brain endothelial cells.
abstract: OBJECTIVE
  Increased amyloid deposition in HIV-infected brains may contribute to the pathogenesis of neurocognitive dysfunction in infected patients. We have previously shown that exposure to HIV results in enhanced amyloid β (Aβ) levels in human brain microvascular endothelial cells, suggesting that brain endothelial cells contribute to accumulation of Aβ in HIV-infected brains. Importantly, Aβ not only accumulates in the cytoplasm of HIV-exposed cells but also enters the nuclei of brain endothelial cells.
  
  
  METHODS
  cDNA microarray analysis was performed in order to examine changes in the transcriptional profile associated with Aβ nuclear entry in the presence of HIV-1.
  
  
  RESULTS
  Gene network analysis indicated that inhibition of nuclear entry of Aβ resulted in enrichment in gene sets involved in apoptosis and survival, endoplasmic reticulum stress response, immune response, cell cycle, DNA damage, oxidative stress, cytoskeleton remodeling and transforming growth factor β (TGFβ) receptor signaling.
  
  
  CONCLUSIONS
  The obtained data indicate that HIV-induced Aβ nuclear uptake affects several cellular stress-related pathways relevant for HIV-induced Aβ pathology.
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Miami School of Medicine, Miami, Florida. Electronic address: iandras@med.miami.edu.
authors.forename: Ibolya E
authors.initials: IE
authors.lastname: András
authors.affiliation: Division of Genetic Epidemiology, Hussman Institute for Human Genomics, Miami, Florida.
authors.forename: Evadnie
authors.initials: E
authors.lastname: Rampersaud
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Miami School of Medicine, Miami, Florida.
authors.forename: Sung Yong
authors.initials: SY
authors.lastname: Eum
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Miami School of Medicine, Miami, Florida.
authors.forename: Michal
authors.initials: M
authors.lastname: Toborek
mesh_terms: D021581:Active Transport, Cell Nucleus / Q000235:genetics*; D016229:Amyloid beta-Peptides / Q000378:metabolism*; D017209:Apoptosis / Q000235:genetics; D001921:Brain / Q000378:metabolism / Q000473:pathology*; D002460:Cell Line; D002478:Cells, Cultured; D034281:Dynamins / Q000037:antagonists & inhibitors; D059865:Endoplasmic Reticulum Stress / Q000235:genetics; D042783:Endothelial Cells / Q000378:metabolism; D057809:HEK293 Cells; D015658:HIV Infections / Q000378:metabolism*; D015497:HIV-1; D006801:Humans; D018384:Oxidative Stress / Q000235:genetics; D021381:Protein Transport / Q000235:genetics*; D015398:Signal Transduction / Q000235:genetics; D016212:Transforming Growth Factor beta / Q000378:metabolism
chemical_list: D016229:Amyloid beta-Peptides; D016212:Transforming Growth Factor beta; D034281:Dynamins
keywords: Amyloid beta; Blood–brain barrier; DNA microarray; HIV