title: From microalgae oil to produce novel structured triacylglycerols enriched with unsaturated fatty acids.
abstract: Novel structured triacylglycerols (STAGs) enriched with unsaturated fatty acids (UFAs) and low palmitic acid (PA) content were firstly synthesized from Schizochytrium sp. oil and oleic acid (OA) via solvent-free acidolysis catalyzed by Lipozyme RM IM. The results indicated that, the PA content decreased from 24.49% to 6.95%, while the UFAs content increased from 70.20% to 90.9% at the sn-1,3 positions in the STAGs under the optimal condition (i.e., lipase load of 7%, molar ratio of microalgae oil TAGs to OA of 1:3, and temperature of 65 °C). The lipase Lipozyme RM IM could be reused 16 times without significant loss of activity. The improved plastic and storage ranges of STAGs are useful for infant formula formulations, by which a possible method is blending of this product and 1,3-dioleoyl-2-palmitoylglycerol enriched fats and minor lipids based on the corresponding chemical compositions of human milk fat.
authors.affiliation: College of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang 212018, PR China; Sericultural Research Institute, Chinese Academy of Agricultural Sciences, Zhenjiang 212018, PR China.
authors.forename: Jun
authors.initials: J
authors.lastname: Wang
authors.affiliation: College of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang 212018, PR China.
authors.forename: Xu-Dong
authors.initials: XD
authors.lastname: Wang
authors.affiliation: College of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang 212018, PR China.
authors.forename: Xing-Yu
authors.initials: XY
authors.lastname: Zhao
authors.affiliation: College of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang 212018, PR China.
authors.forename: Xi
authors.initials: X
authors.lastname: Liu
authors.affiliation: Department of Biological Systems Engineering, Washington State University, Pullman, WA 99164, USA.
authors.forename: Tao
authors.initials: T
authors.lastname: Dong
authors.affiliation: College of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang 212018, PR China; Sericultural Research Institute, Chinese Academy of Agricultural Sciences, Zhenjiang 212018, PR China. Electronic address: fuword@163.com.
authors.forename: Fu-An
authors.initials: FA
authors.lastname: Wu
mesh_terms: D000818:Animals; D003460:Crystallization; D005231:Fatty Acids, Unsaturated / Q000378:metabolism*; D008049:Lipase / Q000378:metabolism; D058086:Microalgae / Q000378:metabolism*; D009821:Oils / Q000378:metabolism*; D019301:Oleic Acid / Q000378:metabolism; D059027:Recycling; D013379:Substrate Specificity; D034421:Sus scrofa; D013696:Temperature; D014280:Triglycerides / Q000096:biosynthesis* / Q000737:chemistry*
chemical_list: D005231:Fatty Acids, Unsaturated; D009821:Oils; D014280:Triglycerides; D019301:Oleic Acid; C093666:Lipozyme; D008049:Lipase
keywords: Biocatalysis; Lipase; Microalgae oil; Polyunsaturated fatty acids; Structured triacylglycerols