title: Insoluble protein applications: the use of bacterial inclusion bodies as biocatalysts.
abstract: Biocatalysis and biotransformations have a broad application in industrial synthetic chemistry. In addition to the whole cell catalysis, purified recombinant enzymes are successfully used for biocatalysis of specific chemical reactions. In this contribution, we report characterization, immobilization, and application of several model target enzymes (D-amino acid oxidase, sialic acid aldolase, maltodextrin phosphorylase, polyphosphate kinase) physiologically aggregated within inclusion bodies (IBs) retaining their biological activity as immobilized biocatalysts.
authors.affiliation: Department of Carbohydrate Enzymology, Laboratory of Synthetic Biology, Institute of Chemistry, Center for Glycomics, Slovak Academy of Sciences, Dúbravská cesta 9, 84538, Bratislava, Slovak Republic.
authors.forename: Eva
authors.initials: E
authors.lastname: Hrabárová
authors.forename: Lucia
authors.initials: L
authors.lastname: Achbergerová
authors.forename: Jozef
authors.initials: J
authors.lastname: Nahálka
mesh_terms: D001419:Bacteria / Q000378:metabolism*; D055162:Biocatalysis; D002384:Catalysis; D002479:Inclusion Bodies / Q000378:metabolism*