title: Effects of culture conditions of Pseudomonas aeruginosa strain RB on the synthesis of CdSe nanoparticles.
abstract: Cadmium selenide (CdSe) was synthesized by Pseudomonas aeruginosa strain RB in a culture containing lactic acid as a carbon source, 1 mM selenite, and 1 mM cadmium under various conditions. High purity (1.02-1.16 of the atomic ratio of Se to Cd) and efficient synthesis of biogenic CdSe nanoparticles were observed at 25-30°C, 0.05-10 g L(-1) NaCl, and neutral pH conditions compared with other tested conditions. However, the size and shape of synthesized CdSe nanoparticles were not changed by changing culture conditions. The contents of S and Se in the particles respectively increased under alkaline and weak acidic conditions. Furthermore, high temperature (>37°C), high salinity (>10 g L(-1) NaCl), and alkaline pH affected the CdSe-synthesizing rate by strain RB. This report is the first optimizing the culture conditions for synthesizing biogenic CdSe nanoparticles in a batch processing.
authors.affiliation: Division of Sustainable Energy and Environmental Engineering, Graduate School of Engineering, Osaka University, 2-1, Yamadaoka, Suita, Osaka 565-0871, Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Ayano
authors.affiliation: Division of Sustainable Energy and Environmental Engineering, Graduate School of Engineering, Osaka University, 2-1, Yamadaoka, Suita, Osaka 565-0871, Japan.
authors.forename: Masashi
authors.initials: M
authors.lastname: Kuroda
authors.affiliation: Division of Sustainable Energy and Environmental Engineering, Graduate School of Engineering, Osaka University, 2-1, Yamadaoka, Suita, Osaka 565-0871, Japan.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Soda
authors.affiliation: Division of Sustainable Energy and Environmental Engineering, Graduate School of Engineering, Osaka University, 2-1, Yamadaoka, Suita, Osaka 565-0871, Japan. Electronic address: ike@see.eng.osaka-u.ac.jp.
authors.forename: Michihiko
authors.initials: M
authors.lastname: Ike
mesh_terms: D061249:Batch Cell Culture Techniques; D002104:Cadmium / Q000494:pharmacology; D019187:Cadmium Compounds / Q000737:chemistry* / Q000378:metabolism*; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D019344:Lactic Acid / Q000378:metabolism / Q000494:pharmacology; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D011550:Pseudomonas aeruginosa / Q000145:classification / Q000254:growth & development* / Q000378:metabolism* / Q000648:ultrastructure; D054712:Salinity; D020887:Selenious Acid / Q000378:metabolism / Q000494:pharmacology; D018036:Selenium Compounds / Q000737:chemistry* / Q000378:metabolism*
chemical_list: D019187:Cadmium Compounds; D018036:Selenium Compounds; D002104:Cadmium; D019344:Lactic Acid; C058667:cadmium selenide; D020887:Selenious Acid
keywords: Cadmium resistance; Cadmium selenide nanoparticles; Optimization; Pseudomonas aeruginosa; Selenite reduction