title: Safety assessment of dietary bamboo charcoal powder: a 90-day subchronic oral toxicity and mutagenicity studies.
abstract: Vegetable carbon has been used as food additive in EU (E153) and China for many years; however, no experimental data have been available on its dietary safety. This study was designed to evaluate the subchronic toxicity and genotoxicity of bamboo charcoal powder (BCP). In the study of subchronic oral toxicity, BCP was administered orally at doses of 2.81, 5.62, and 11.24 g/kg BW for 90 days to SD rats. Additional satellite groups from the control group and high dose group were observed for a 28-day recovery period. At the end of the treatment and recovery periods, animals were sacrificed, and their organs were weighed and blood samples were collected. The toxicological endpoints observed included clinical signs, food consumption, body and organ weights, hematological and biochemical parameters, macroscopic and microscopic examinations. The results showed no significant differences between the BCP treated groups and control group. The genotoxicity of BCP was assessed with the Salmonella typhimurium mutagenicity assay (Ames test) and a combination of comet assay and mammalian erythrocyte micronucleus protocol. The results did not reveal any genotoxicity of BCP. Based on our study, the no-observed-adverse-effect level (NOAEL) for BCP is 11.24 g/kg BW/day.
authors.affiliation: West China School of Public Health, Sichuan University, No. 16, third section, South Renmin Road, Chengdu, Sichuan 610041, China.
authors.forename: Jia
authors.initials: J
authors.lastname: Zhenchao
authors.affiliation: West China School of Public Health, Sichuan University, No. 16, third section, South Renmin Road, Chengdu, Sichuan 610041, China.
authors.forename: Zhong
authors.initials: Z
authors.lastname: Yuting
authors.affiliation: West China School of Public Health, Sichuan University, No. 16, third section, South Renmin Road, Chengdu, Sichuan 610041, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Jiuming
authors.affiliation: West China School of Public Health, Sichuan University, No. 16, third section, South Renmin Road, Chengdu, Sichuan 610041, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Yedan
authors.affiliation: West China School of Public Health, Sichuan University, No. 16, third section, South Renmin Road, Chengdu, Sichuan 610041, China.
authors.forename: Song
authors.initials: S
authors.lastname: Yang
authors.affiliation: West China School of Public Health, Sichuan University, No. 16, third section, South Renmin Road, Chengdu, Sichuan 610041, China; Food Safety Monitoring and Risk Assessment Key Laboratory of Sichuan Province, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Jinyao
authors.affiliation: West China School of Public Health, Sichuan University, No. 16, third section, South Renmin Road, Chengdu, Sichuan 610041, China; Food Safety Monitoring and Risk Assessment Key Laboratory of Sichuan Province, China. Electronic address: lishizhang_56@163.com.
authors.forename: Zhang
authors.initials: Z
authors.lastname: Lishi
mesh_terms: D000284:Administration, Oral; D000818:Animals; D031723:Bambusa; D001835:Body Weight / Q000187:drug effects; D002606:Charcoal / Q000633:toxicity*; D020552:Comet Assay; D004249:DNA Damage / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005260:Female; D005503:Food Additives / Q000633:toxicity; D008297:Male; D015162:Micronucleus Tests; D009153:Mutagens / Q000633:toxicity; D019055:No-Observed-Adverse-Effect Level; D009929:Organ Size / Q000187:drug effects; D011208:Powders; D051381:Rats; D017207:Rats, Sprague-Dawley; D018570:Risk Assessment; D012486:Salmonella typhimurium; D059550:Toxicity Tests, Subchronic
chemical_list: D005503:Food Additives; D009153:Mutagens; D011208:Powders; D002606:Charcoal
keywords: 90-day subchronic oral toxicity; Ames test; Bamboo charcoal powder; Comet assay; Micronucleus test