title: Practical method for the confirmation of authentic flours of different types of cereals and pseudocereals.
abstract: Gas chromatography with mass spectrometry was used to perform a qualitative analysis of the liposoluble flour extract of different types of cereals (bread wheat and spelt) and pseudocereals (amaranth and buckwheat). In addition to major fatty acids, the liposoluble extract also contained minor fatty acids with more than 20 carbon atoms, higher hydrocarbons and phytosterols. TMSH (trimethylsulfonium hydroxide, 0.2 mol/l in methanol) was used as a trans-esterification reagent. In a trans-esterification reaction, triglycerides esterified from acilglycerols to methyl-esters. SIM (selected ion monitoring) was applied to isolate fatty acid methyl esters on TIC (total ion current) chromatograms, using the 74 Da fragment ion, which originated from McLafferty rearrangement, and is typical for methyl-esters. GC-MS system was used for the trans-esterification of triglycerides to fatty acid methyl esters in the gas chromatographic injector. This eliminated laboratory preparation for fatty acid methyl esters. Cluster analysis was applied to compare the liposoluble flour extract from different types of cereals and pseudocereals. Statistical data showed the liposoluble extract analysis enabled determination of flour origin and, because the results were unambiguous, this approach could be used for quality control.
authors.affiliation: Faculty of Technology, University of Novi Sad, Bulevar Cara Lazara 1, Novi Sad, Serbia. Electronic address: macanski@tf.uns.ac.rs.
authors.forename: Marijana M
authors.initials: MM
authors.lastname: Ačanski
authors.affiliation: Institute for Food Technology Novi Sad, University of Novi Sad, Bulevar Cara Lazara 1, Novi Sad, Serbia. Electronic address: djuravujic@gmail.com.
authors.forename: Djura N
authors.initials: DN
authors.lastname: Vujić
authors.affiliation: Institute for Food Technology Novi Sad, University of Novi Sad, Bulevar Cara Lazara 1, Novi Sad, Serbia. Electronic address: djordje.psodorov@fins.uns.ac.rs.
authors.forename: Djordje B
authors.initials: DB
authors.lastname: Psodorov
mesh_terms: D002523:Edible Grain / Q000737:chemistry*; D004951:Esterification; D005227:Fatty Acids / Q000032:analysis; D005433:Flour / Q000032:analysis*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*
chemical_list: D005227:Fatty Acids
keywords: Cereals and pseudocereals; Correlations of liposoluble composition; GC–MS; Methanol (PubChem CID: 887); Trimethylsulfonium hydroxide, TMSH (PubChem CID: 11105313); n-Hexane (PubChem CID: 8058)