title: Comparative analysis of phytochemicals and nutrient availability in two contrasting cultivars of sweet potato (Ipomoea batatas L.).
abstract: Sweet potato ranks as the world's seventh most important food crop, and has major contribution to energy and phytochemical source of nutrition. To unravel the molecular basis for differential nutrient availability, and to exploit the natural genetic variation(s) of sweet potato, a series of physiochemical and proteomics experiment was conducted using two contrasting cultivars, an orange-fleshed sweet potato (OFSP) and a white-fleshed sweet potato (WFSP). Phytochemical screening revealed high percentage of carbohydrate, reducing sugar and phenolics in WFSP, whereas OFSP showed increased levels of total protein, flavonoids, anthocyanins, and carotenoids. The rate of starch and cellulose degradation was found to be less in OFSP during storage, indicating tight regulation of gene(s) responsible for starch-degradation. Comparative proteomics displayed a cultivar-dependent expression of proteins along with evolutionarily conserved proteins. These results suggest that cultivar-specific expression of proteins and/or their interacting partners might play a crucial role for nutrient acquisition in sweet potato.
authors.affiliation: National Institute of Plant Genome Research, Jawaharlal Nehru University Campus, Aruna Asaf Ali Marg, New Delhi 110067, India; Department of Molecular Biology and Biotechnology, Tezpur University, Assam, India.
authors.forename: Shubhendu
authors.initials: S
authors.lastname: Shekhar
authors.affiliation: National Institute of Plant Genome Research, Jawaharlal Nehru University Campus, Aruna Asaf Ali Marg, New Delhi 110067, India.
authors.forename: Divya
authors.initials: D
authors.lastname: Mishra
authors.affiliation: Department of Molecular Biology and Biotechnology, Tezpur University, Assam, India.
authors.forename: Alak Kumar
authors.initials: AK
authors.lastname: Buragohain
authors.affiliation: National Institute of Plant Genome Research, Jawaharlal Nehru University Campus, Aruna Asaf Ali Marg, New Delhi 110067, India. Electronic address: subhrac@hotmail.com.
authors.forename: Subhra
authors.initials: S
authors.lastname: Chakraborty
authors.affiliation: National Institute of Plant Genome Research, Jawaharlal Nehru University Campus, Aruna Asaf Ali Marg, New Delhi 110067, India. Electronic address: nchakraborty@nipgr.ac.in.
authors.forename: Niranjan
authors.initials: N
authors.lastname: Chakraborty
mesh_terms: D000872:Anthocyanins / Q000737:chemistry* / Q000378:metabolism; D002338:Carotenoids / Q000737:chemistry* / Q000378:metabolism; D027723:Ipomoea batatas / Q000737:chemistry* / Q000378:metabolism; D009753:Nutritive Value; D010636:Phenols / Q000737:chemistry / Q000378:metabolism; D064209:Phytochemicals / Q000737:chemistry / Q000378:metabolism; D014801:Vitamin A / Q000378:metabolism
chemical_list: D000872:Anthocyanins; D010636:Phenols; D064209:Phytochemicals; D014801:Vitamin A; D002338:Carotenoids
keywords: Anthocyanins; Flavonoids; Nutritional diversity; Phenolics; Phytochemicals; Proteome profiling; Storage stress; Transcript abundance