title: Straightforward method to quantify GSH, GSSG, GRP, and hydroxycinnamic acids in wines by UPLC-MRM-MS.
abstract: A novel, robust and fast ultrahigh performance liquid chromatography–multiple reaction monitoring mass spectrometry method has been developed for the simultaneous quantification of reduced glutathione (GSH), oxidized glutathione (GSSG), grape reaction product (GRP) and hydroxycinnamic acids in wine. The method was evaluated in terms of linearity, precision, accuracy, limits of detection and quantification, stability and matrix effects. Quantitative recovery (74–110%) and satisfactory interday precision (RSD <14%) were achieved for all target compounds. No significant matrix-dependent suppression/enhancement effects were observed. To demonstrate the method applicability, white, rosé and red wine samples with different levels of oxidation were analyzed. Levels of GSH and GSSG varied from 1.63 to 9.91 mg/L and from 0.32 to 3.33 mg/L, respectively. Levels of caftaric acid ranged from 2.57 to 293.07 mg/L. Levels of GRP were between 28.35 and 114.20 mg/L.
authors.forename: Anna
authors.initials: A
authors.lastname: Vallverdú-Queralt
authors.forename: Arnaud
authors.initials: A
authors.lastname: Verbaere
authors.forename: Emmanuelle
authors.initials: E
authors.lastname: Meudec
authors.forename: Veronique
authors.initials: V
authors.lastname: Cheynier
authors.forename: Nicolas
authors.initials: N
authors.lastname: Sommerer
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003373:Coumaric Acids / Q000032:analysis*; D004355:Drug Stability; D005978:Glutathione / Q000032:analysis*; D019803:Glutathione Disulfide / Q000032:analysis*; D006010:Glycogen Storage Disease Type III; D013058:Mass Spectrometry / Q000379:methods; D010636:Phenols / Q000032:analysis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014920:Wine / Q000032:analysis*
chemical_list: D003373:Coumaric Acids; D010636:Phenols; D005978:Glutathione; D019803:Glutathione Disulfide; C515101:caftaric acid