title: Melittin disruption of raft and non-raft-forming biomimetic membranes: a study by quartz crystal microbalance with dissipation monitoring.
abstract: In this work we examine the role of lateral phase separation in cholesterol-containing biomimetic membranes on the disrupting action of melittin using a label-free surface-sensitive technique, quartz crystal microbalance with dissipation monitoring (QCM-D). Melittin disruption mechanisms depend strongly on the geometry of the lipid layer; however, despite the interplay between layer geometry/thickness and melittin activity, results indicate that the presence of lipid heterogeneity and lateral phase separation greatly influences the disrupting efficiency of melittin. In homogeneous non-raft forming membranes with high cholesterol content, melittin spontaneous activity is strongly delayed compared to heterogeneous raft-forming systems with the same amount of cholesterol. These results confirm the importance of lateral phase separation as a determinant factor in peptide activity. The information provided can be used for the design of more efficient antimicrobial peptides and the possibility of using a label-free approach for tailored-membranes and interactions with other types of peptides, such as amyloid peptides.
authors.affiliation: Institute for Materials Research IMO, Hasselt University, Wetenschapspark 1, B-3590 Diepenbeek, Belgium; IMEC vzw, Division IMOMEC, Wetenschapspark 1, B-3590 Diepenbeek, Belgium. Electronic address: patricia.losadaperez@uhasselt.be.
authors.forename: P
authors.initials: P
authors.lastname: Losada-Pérez
authors.affiliation: Institute for Materials Research IMO, Hasselt University, Wetenschapspark 1, B-3590 Diepenbeek, Belgium.
authors.forename: M
authors.initials: M
authors.lastname: Khorshid
authors.affiliation: Institute for Materials Research IMO, Hasselt University, Wetenschapspark 1, B-3590 Diepenbeek, Belgium.
authors.forename: C
authors.initials: C
authors.lastname: Hermans
authors.affiliation: Institute for Materials Research IMO, Hasselt University, Wetenschapspark 1, B-3590 Diepenbeek, Belgium.
authors.forename: T
authors.initials: T
authors.lastname: Robijns
authors.affiliation: Institute for Materials Research IMO, Hasselt University, Wetenschapspark 1, B-3590 Diepenbeek, Belgium.
authors.forename: M
authors.initials: M
authors.lastname: Peeters
authors.affiliation: Institute for Materials Research IMO, Hasselt University, Wetenschapspark 1, B-3590 Diepenbeek, Belgium.
authors.forename: K L
authors.initials: KL
authors.lastname: Jiménez-Monroy
authors.affiliation: School of Engineering Physics, Hanoi University of Science and Technology 1, Dai Co Viet, Hanoi, Vietnam.
authors.forename: L T N
authors.initials: LT
authors.lastname: Truong
authors.affiliation: Institute for Materials Research IMO, Hasselt University, Wetenschapspark 1, B-3590 Diepenbeek, Belgium; IMEC vzw, Division IMOMEC, Wetenschapspark 1, B-3590 Diepenbeek, Belgium.
authors.forename: P
authors.initials: P
authors.lastname: Wagner
mesh_terms: D032701:Biomimetics / Q000379:methods*; D002784:Cholesterol / Q000737:chemistry*; D008555:Melitten / Q000737:chemistry*; D008567:Membranes, Artificial; D058525:Quartz Crystal Microbalance Techniques / Q000379:methods*
chemical_list: D008567:Membranes, Artificial; D008555:Melitten; D002784:Cholesterol
keywords: Cholesterol; Lipid rafts; Melittin; Quartz crystal microbalance with dissipation