title: Preparation and optimization of ophiopogon polysaccharide liposome and its activity on Kupffer cells.
abstract: The purpose of this study was to prepare and optimize ophiopogon polysaccharide liposome (OPL), and to improve the immune-enhancing activity of ophiopogon polysaccharide (OP). OPL was prepared and optimized using the methods of reverse-phase evaporation and response surface methodology. The property was evaluated with particle size, zeta potential, and morphology. The results showed that the optimum preparation conditions were: soybean phosphatide to OP ratio of 9.5:1, soybean phospholipid to cholesterol ratio of 8:1, and chloroform to phosphate-buffered saline ratio of 3:1. Subsequently, the immune-enhancing activity of OPL on Kupffer cells (KCs) was performed. The results showed that OPL could significantly promote the phagocytosis of KCs, induce the secretion of nitric oxide, induced nitric oxide synthase, IL-6 and IL-12, and improve the expression of CD80 and CD86 compared with OP at 125-7.813 μg mL(-1). These results indicated that the immune-enhancing activity of OP was significantly improved after encapsulated with liposome. Therefore, liposome would be expected to exploit into a new-type preparation of OP.
authors.affiliation: College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi 712100, PR China.
authors.forename: Yunpeng
authors.initials: Y
authors.lastname: Fan
authors.affiliation: College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi 712100, PR China.
authors.forename: Xiaoping
authors.initials: X
authors.lastname: Song
authors.affiliation: College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi 712100, PR China.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Gao
authors.affiliation: College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi 712100, PR China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Chen
authors.affiliation: College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi 712100, PR China.
authors.forename: Lin
authors.initials: L
authors.lastname: Ma
authors.affiliation: College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi 712100, PR China.
authors.forename: Weimin
authors.initials: W
authors.lastname: Zhang
authors.affiliation: College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi 712100, PR China.
authors.forename: Weifeng
authors.initials: W
authors.lastname: Hou
authors.affiliation: College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi 712100, PR China.
authors.forename: Chao
authors.initials: C
authors.lastname: Guo
authors.affiliation: College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi 712100, PR China. Electronic address: dwtong@nwsuaf.edu.cn.
authors.forename: Dewen
authors.initials: D
authors.lastname: Tong
mesh_terms: D000818:Animals; D018122:B7-1 Antigen / Q000235:genetics; D051940:B7-2 Antigen / Q000235:genetics; D002478:Cells, Cultured; D002626:Chemistry, Pharmaceutical; D004339:Drug Compounding; D007155:Immunologic Factors / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*; D018664:Interleukin-12 / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D007728:Kupffer Cells / Q000187:drug effects* / Q000276:immunology; D008081:Liposomes; D008516:Medicine, Chinese Traditional; D051379:Mice; D009569:Nitric Oxide / Q000378:metabolism; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism; D031441:Ophiopogon / Q000737:chemistry*; D010316:Particle Size; D010587:Phagocytosis / Q000187:drug effects* / Q000276:immunology; D011134:Polysaccharides / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*; D051381:Rats; D013499:Surface Properties
chemical_list: D018122:B7-1 Antigen; D051940:B7-2 Antigen; C497966:Cd86 protein, rat; D007155:Immunologic Factors; D015850:Interleukin-6; D008081:Liposomes; D011134:Polysaccharides; D018664:Interleukin-12; D009569:Nitric Oxide; D052247:Nitric Oxide Synthase Type II; C496318:Nos2 protein, rat
keywords: Immune-enhancing activity; Kupffer cells; Ophiopogon polysaccharide liposome; Response surface methodology