title: α-Synemin localizes to the M-band of the sarcomere through interaction with the M10 region of titin.
abstract: α-Synemin contains a unique 312 amino acid insert near the end of its C-terminal tail. Therefore we set out to determine if the insert is a site of protein-protein interaction that regulates the sub-cellular localization of this large isoform of synemin. Yeast-two hybrid analysis indicated that this region is a binding site for the M10 region of titin. This was confirmed with GST pull-down assays. Co-immunoprecipitation of endogenous proteins indicated close association of the two proteins in vivo and immunostaining of cardiomyocytes demonstrated co-localization of the proteins at the M-band of the sarcomere.
authors.affiliation: School of Biomedical Sciences, Kent State University, United States.
authors.forename: Bethany C
authors.initials: BC
authors.lastname: Prudner
authors.affiliation: Department of Biological Science, Kent State University, United States.
authors.forename: Pritam Sinha
authors.initials: PS
authors.lastname: Roy
authors.affiliation: School of Biomedical Sciences, Department of Biological Science, Kent State University, United States.
authors.forename: Derek S
authors.initials: DS
authors.lastname: Damron
authors.affiliation: School of Biomedical Sciences, Department of Biological Science, Kent State University at Trumbull, United States. Electronic address: mrussel1@kent.edu.
authors.forename: Mary A
authors.initials: MA
authors.lastname: Russell
mesh_terms: D000818:Animals; D002460:Cell Line; D064211:Connectin / Q000737:chemistry* / Q000378:metabolism*; D006801:Humans; D007381:Intermediate Filament Proteins / Q000737:chemistry / Q000378:metabolism*; D042541:Intracellular Space / Q000378:metabolism; D051379:Mice; D011485:Protein Binding; D021381:Protein Transport; D012518:Sarcomeres / Q000378:metabolism*
chemical_list: D064211:Connectin; D007381:Intermediate Filament Proteins; C430456:desmuslin
keywords: A-kinase anchoring protein; Cytoskeleton; Intermediate filament; Protein kinase A; Signal transduction; Synemin