title: The haemagglutination activity of equine herpesvirus type 1 glycoprotein C.
abstract: Equine herpesvirus type 1 (EHV-1) has haemagglutination (HA) activity toward equine red blood cells (RBCs), but the identity of its haemagglutinin is unknown. To identify the haemagglutinin of EHV-1, the major glycoproteins of EHV-1 were expressed in 293T cells, and the cells or cell lysates were mixed with equine RBCs. The results showed that only EHV-1 glycoprotein C (gC)-producing cells adsorbed equine RBCs, and that the lysate of EHV-1 gC-expressing cells agglutinated equine RBCs. EHV-1 lacking gC did not show HA activity. HA activity was inhibited by monoclonal antibodies (MAbs) specific for gC, but not by antibodies directed against other glycoproteins. In addition, HA activity was not inhibited by the addition of heparin. These results indicate that EHV-1 gC can bind equine RBCs irrespective of heparin, in contrast to other herpesvirus gC proteins.
authors.affiliation: Laboratory of Veterinary Microbiology, Joint Faculty of Veterinary Medicine, Yamaguchi University, 1677-1 Yoshida, Yamaguchi 753-8515, Japan.
authors.forename: Kiyohiko
authors.initials: K
authors.lastname: Andoh
authors.affiliation: Laboratory of Veterinary Microbiology, Joint Faculty of Veterinary Medicine, Yamaguchi University, 1677-1 Yoshida, Yamaguchi 753-8515, Japan.
authors.forename: Shiho
authors.initials: S
authors.lastname: Hattori
authors.affiliation: Laboratory of Veterinary Microbiology, Joint Faculty of Veterinary Medicine, Yamaguchi University, 1677-1 Yoshida, Yamaguchi 753-8515, Japan.
authors.forename: Hassan Y A H
authors.initials: HY
authors.lastname: Mahmoud
authors.affiliation: Laboratory of Veterinary Microbiology, Joint Faculty of Veterinary Medicine, Yamaguchi University, 1677-1 Yoshida, Yamaguchi 753-8515, Japan.
authors.forename: Maaya
authors.initials: M
authors.lastname: Takasugi
authors.affiliation: Laboratory of Veterinary Microbiology, Joint Faculty of Veterinary Medicine, Yamaguchi University, 1677-1 Yoshida, Yamaguchi 753-8515, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Shimoda
authors.affiliation: Molecular Biology Division, Epizootic Research Center, Equine Research Institute, Japan Racing Association, Shimotsuke, Tochigi 329-0412, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Bannai
authors.affiliation: Molecular Biology Division, Epizootic Research Center, Equine Research Institute, Japan Racing Association, Shimotsuke, Tochigi 329-0412, Japan.
authors.forename: Koji
authors.initials: K
authors.lastname: Tsujimura
authors.affiliation: Molecular Biology Division, Epizootic Research Center, Equine Research Institute, Japan Racing Association, Shimotsuke, Tochigi 329-0412, Japan.
authors.forename: Tomio
authors.initials: T
authors.lastname: Matsumura
authors.affiliation: Molecular Biology Division, Epizootic Research Center, Equine Research Institute, Japan Racing Association, Shimotsuke, Tochigi 329-0412, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Kondo
authors.affiliation: Department of Veterinary Virology, Rakuno Gakuen University, Ebetsu, Hokkaido 069-0836, Japan.
authors.forename: Rikio
authors.initials: R
authors.lastname: Kirisawa
authors.affiliation: Laboratory of Emerging Infectious Diseases, Joint Faculty of Veterinary Medicine, Kagoshima University, 1-21-24 Korimoto, Kagoshima 890-0065, Japan.
authors.forename: Masami
authors.initials: M
authors.lastname: Mochizuki
authors.affiliation: Laboratory of Veterinary Microbiology, Joint Faculty of Veterinary Medicine, Yamaguchi University, 1677-1 Yoshida, Yamaguchi 753-8515, Japan. Electronic address: kmaeda@yamaguchi-u.ac.jp.
authors.forename: Ken
authors.initials: K
authors.lastname: Maeda
mesh_terms: D000818:Animals; D004912:Erythrocytes / Q000187:drug effects; D006384:Hemagglutination; D006388:Hemagglutinins / Q000378:metabolism*; D004861:Herpesvirus 1, Equid / Q000502:physiology*; D006736:Horses; D014759:Viral Envelope Proteins / Q000378:metabolism*
chemical_list: D006388:Hemagglutinins; D014759:Viral Envelope Proteins; C080410:glycoprotein C, Equid herpesvirus 1
keywords: Equine herpesvirus type 1; Glycoprotein C; Haemagglutination activity; Heparin