title: Detection of circulating tumor cells in prostate cancer based on carboxylated graphene oxide modified light addressable potentiometric sensor.
abstract: Circulating tumor cells (CTCs) are a group of rare cancer cells that have detached from a primary tumor and circulate in the bloodstream. Herein, light addressable potentiometric sensor (LAPS) was exploited in the label-free detection of CTCs in the prostate cancer. To this end, the mouse anti-human epithelial cell adhesion molecule (anti-EpCAM) monoclonal antibody was selected as the probe to capture CTCs according to our western blot experiments, and therefore the anti-EpCAM was immobilized on the surface of carboxylated graphene oxide (GO-COOH) modified LAPS. Spiking experiments confirmed that LAPS' voltage decreased with the increasing of CTCs' concentration both in phosphate buffer (PBS) and blood, and as few as 10 CTCs in 1ml of blood could be detected, illustrating the high sensitivity of the proposed strategy. The analysis of healthy blood samples revealed no change in electrical signal, confirming the specificity of the system. Ultraviolet-visible (UV-vis) spectroscopy, scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS) and immunofluorescent assay (IFA) were conducted to characterize GO-COOH, testify its existence on LAPS and validate CTCs' capturing by anti-EpCAM grafted on GO-COOH modified substrates. It is indicated that LAPS could be a potential platform for CTCs detection and may provide a powerful tool for downstream analysis.
authors.affiliation: School of Medical Laboratory, Tianjin Medical University, No. 1 Guangdong Road, Hexi District, Tianjin 300203, PR China.
authors.forename: Yajun
authors.initials: Y
authors.lastname: Gu
authors.affiliation: College of Electronic Information and Optical Engineering, Nankai University, No. 94 Weijin Road, Nankai District, Tianjin 300071, PR China.
authors.forename: Cheng
authors.initials: C
authors.lastname: Ju
authors.affiliation: Department of Urology, Second Hospital of Tianjin Medical University, Tianjin Institute of Urology, No. 23 Pingjiang Road, Tianjin, PR China.
authors.forename: Yanjun
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Urology, Second Hospital of Tianjin Medical University, Tianjin Institute of Urology, No. 23 Pingjiang Road, Tianjin, PR China.
authors.forename: Zhiqun
authors.initials: Z
authors.lastname: Shang
authors.affiliation: Department of Urology, Second Hospital of Tianjin Medical University, Tianjin Institute of Urology, No. 23 Pingjiang Road, Tianjin, PR China.
authors.forename: Yudong
authors.initials: Y
authors.lastname: Wu
authors.affiliation: College of Electronic Information and Optical Engineering, Nankai University, No. 94 Weijin Road, Nankai District, Tianjin 300071, PR China. Electronic address: jiayf@nankai.edu.cn.
authors.forename: Yunfang
authors.initials: Y
authors.lastname: Jia
authors.affiliation: Department of Urology, Second Hospital of Tianjin Medical University, Tianjin Institute of Urology, No. 23 Pingjiang Road, Tianjin, PR China. Electronic address: niuyuanjie9317@163.com.
authors.forename: Yuanjie
authors.initials: Y
authors.lastname: Niu
mesh_terms: D000328:Adult; D015374:Biosensing Techniques / Q000295:instrumentation*; D002264:Carboxylic Acids / Q000737:chemistry; D045744:Cell Line, Tumor; D006108:Graphite / Q000737:chemistry*; D006801:Humans; D008027:Light; D008297:Male; D008875:Middle Aged; D009360:Neoplastic Cells, Circulating / Q000473:pathology*; D010087:Oxides / Q000737:chemistry*; D011199:Potentiometry / Q000295:instrumentation*; D011467:Prostate / Q000473:pathology; D011471:Prostatic Neoplasms / Q000097:blood* / Q000473:pathology; D055815:Young Adult
chemical_list: D002264:Carboxylic Acids; D010087:Oxides; D006108:Graphite
keywords: Carboxylated graphene oxide; Circulating tumor cells (CTCs); Light addressable potentiometric sensor (LAPS); Prostate cancer