title: Effects of blue light phototherapy on DNA integrity in preterm newborns.
abstract: In previous studies, exposure to phototherapy, but not oxygen therapy, resulted in damage to genetic material in newborns. The objective of this study was to determine whether micronucleated erythrocytes (MNE) increased in preterm newborns (PNBs) who were exposed to blue light phototherapy lamps. MNE of mature organisms are rapidly eliminated by the spleen, and the presence of MNE has been related to immaturity in some species. Furthermore, PNBs present spontaneous MNE. Blood samples were taken from 17 PNBs at birth to establish baseline frequencies (0 h). After beginning blue light phototherapy, blood samples were obtained from 11 of these PNBs at 24-h intervals for 96 h, after the baseline sample. MNE and micronucleated polychromatic erythrocytes (MNPCE) were counted. The basal values of MNE and MNPCE from 17 PNBs were 0.62 ± 0.48 and 1.52 ± 1.28 (‰), respectively, and no increase in MNE or MNPCE was observed in the serial samples of 11 PNBs exposed to blue light and oxygen therapies, though previous studies reported increases using other types of lamps. In conclusion, under the conditions described no increase in the number of MNE or MNPCE was observed in the peripheral blood of PNBs exposed to blue light phototherapy.
authors.affiliation: Instituto de Biología Molecular en Medicina, Departamento de Biología Molecular y Genómica, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Guadalajara, Jalisco, Mexico.
authors.forename: Belinda C
authors.initials: BC
authors.lastname: Gómez-Meda
authors.affiliation: Laboratorio de Mutagénesis, Centro de Investigación Biomédica de Occidente, Instituto Mexicano del Seguro Social, Guadalajara, Jalisco, Mexico; Servicio de Neonatología del Hospital Infantil de México Federico Gómez, México, Distrito Federal, Mexico.
authors.forename: Angélica
authors.initials: A
authors.lastname: Barros-Hernández
authors.affiliation: Servicio de Neonatología del Hospital Infantil de México Federico Gómez, México, Distrito Federal, Mexico.
authors.forename: José
authors.initials: J
authors.lastname: Guzmán-Bárcenas
authors.affiliation: Departamento de Neonatología, Hospital de Pediatría, Unidad Médica de Alta Especialidad, Centro Médico Nacional de Occidente, Instituto Mexicano del Seguro Social, Guadalajara, Jalisco, Mexico.
authors.forename: María de Lourdes
authors.initials: Mde L
authors.lastname: Lemus-Varela
authors.affiliation: Instituto de Investigación en Odontología, Departamento de Clínicas Odontológicas Integrales, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Guadalajara, Jalisco, Mexico.
authors.forename: Ana L
authors.initials: AL
authors.lastname: Zamora-Perez
authors.affiliation: División de Neurociencias, Centro de Investigación Biomédica de Occidente, Instituto Mexicano del Seguro Social, Guadalajara, Jalisco, Mexico; Departamento de Clínicas Médicas, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Guadalajara, Jalisco, Mexico.
authors.forename: Blanca M
authors.initials: BM
authors.lastname: Torres-Mendoza
authors.affiliation: Laboratorio de Genética Molecular, Centro de Investigación Biomédica de Occidente, Instituto Mexicano del Seguro Social, Guadalajara, Jalisco, Mexico.
authors.forename: Martha P
authors.initials: MP
authors.lastname: Gallegos-Arreola
authors.affiliation: Instituto de Biología Molecular en Medicina, Departamento de Biología Molecular y Genómica, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Guadalajara, Jalisco, Mexico.
authors.forename: Juan
authors.initials: J
authors.lastname: Armendáriz-Borunda
authors.affiliation: Laboratorio de Mutagénesis, Centro de Investigación Biomédica de Occidente, Instituto Mexicano del Seguro Social, Guadalajara, Jalisco, Mexico. Electronic address: mutagenesis95@hotmail.com.
authors.forename: Guillermo M
authors.initials: GM
authors.lastname: Zúñiga-González
mesh_terms: D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D004912:Erythrocytes / Q000166:cytology / Q000528:radiation effects; D005260:Female; D005865:Gestational Age; D006801:Humans; D006931:Hyperbaric Oxygenation; D006932:Hyperbilirubinemia / Q000628:therapy; D007231:Infant, Newborn; D007234:Infant, Premature; D008027:Light; D008297:Male; D010789:Phototherapy
chemical_list: D004247:DNA