title: Precision breeding of grapevine (Vitis vinifera L.) for improved traits.
abstract: This review provides an overview of recent technological advancements that enable precision breeding to genetically improve elite cultivars of grapevine (Vitis vinifera L.). Precision breeding, previously termed "cisgenic" or "intragenic" genetic improvement, necessitates a better understanding and use of genomic resources now becoming accessible. Although it is now a relatively simple task to identify genetic elements and genes from numerous "omics" databases, the control of major agronomic and enological traits often involves the currently unknown participation of many genes and regulatory machineries. In addition, genetic evolution has left numerous vestigial genes and sequences without tangible functions. Thus, it is critical to functionally test each of these genetic entities to determine their real-world functionality or contribution to trait attributes. Toward this goal, several diverse techniques now are in place, including cell culture systems to allow efficient plant regeneration, advanced gene insertion techniques, and, very recently, resources for genomic analyses. Currently, these techniques are being used for high-throughput expression analysis of a wide range of grapevine-derived promoters and disease-related genes. It is envisioned that future research efforts will be extended to the study of promoters and genes functioning to enhance other important traits, such as fruit quality and vigor.
authors.affiliation: Grape Biotechnology Core Laboratory, Mid-Florida Research and Education Center, University of Florida/IFAS, 2725 Binion Road, Apopka, FL 32703-8504 USA. Electronic address: djg@ufl.edu.
authors.forename: Dennis J
authors.initials: DJ
authors.lastname: Gray
authors.affiliation: Grape Biotechnology Core Laboratory, Mid-Florida Research and Education Center, University of Florida/IFAS, 2725 Binion Road, Apopka, FL 32703-8504 USA.
authors.forename: Zhijian T
authors.initials: ZT
authors.lastname: Li
authors.affiliation: Department of Plant Sciences, Sheridan Research and Extension Center, University of Wyoming, 663 Wyarno Road, Sheridan, WY 82801 USA.
authors.forename: Sadanand A
authors.initials: SA
authors.lastname: Dhekney
mesh_terms: D001947:Breeding / Q000379:methods*; D017343:Genes, Plant; D016254:Mutagenesis, Insertional; D011401:Promoter Regions, Genetic; D027843:Vitis / Q000235:genetics*
keywords: Anthocyanin marker; Cisgenics; Disease resistance; Expression analysis; Genetic engineering; Grapevine; Intragenics; Vitis vinifera