title: Three-dimensional printed strontium-containing mesoporous bioactive glass scaffolds for repairing rat critical-sized calvarial defects.
abstract: The development of a new generation of biomaterials with high osteogenic ability for fast osseointegration with host bone is being intensively investigated. In this study, we have fabricated three-dimensional (3-D) strontium-containing mesoporous bioactive glass (Sr-MBG) scaffolds by a 3-D printing technique. Sr-MBG scaffolds showed uniform interconnected macropores (∼400μm), high porosity (∼70%) and enhanced compressive strength (8.67±1.74MPa). Using MBG scaffolds as a control, the biological properties of Sr-MBG scaffolds were evaluated by apatite-forming ability, adhesion, proliferation, alkaline phosphatase activity and osteogenic gene expression of osteoblast-like cells MC3T3-E1. Furthermore, Sr-MBG scaffolds were used to repair critical-sized rat calvarial defects. The results showed that Sr-MBG scaffolds possessed good apatite-forming ability and stimulated MC3T3-E1 cell proliferation and differentiation. Importantly, the in vivo results revealed that Sr-MBG scaffolds had good osteogenic capability and stimulated new blood vessel formation in critical-sized rat calvarial defects within 8 weeks. Therefore, 3-D printed Sr-MBG scaffolds with favorable pore structure and high osteogenic ability have more potential applications in bone regeneration.
authors.affiliation: Department of Orthopedics, Shanghai Sixth People's Hospital, Shanghai Jiao Tong University, 600 Yishan Road, Shanghai 200233, People's Republic of China.
authors.forename: Shichang
authors.initials: S
authors.lastname: Zhao
authors.affiliation: School of Medical Instrument and Food Engineering, University of Shanghai for Science and Technology, 516 Jungong Road, Shanghai 200093, People's Republic of China; School of Materials Science and Engineering, University of Shanghai for Science and Technology, 516 Jungong Road, Shanghai 200093, People's Republic of China.
authors.forename: Jianhua
authors.initials: J
authors.lastname: Zhang
authors.affiliation: School of Materials Science and Engineering, University of Shanghai for Science and Technology, 516 Jungong Road, Shanghai 200093, People's Republic of China.
authors.forename: Min
authors.initials: M
authors.lastname: Zhu
authors.affiliation: Department of Orthopedics, Shanghai Sixth People's Hospital, Shanghai Jiao Tong University, 600 Yishan Road, Shanghai 200233, People's Republic of China.
authors.forename: Yadong
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Orthopedics, Changhai Hospital, Second Military Medical University, 174 Changhai Road, Shanghai 200433, People's Republic of China.
authors.forename: Zhongtang
authors.initials: Z
authors.lastname: Liu
authors.affiliation: School of Medical Instrument and Food Engineering, University of Shanghai for Science and Technology, 516 Jungong Road, Shanghai 200093, People's Republic of China; School of Materials Science and Engineering, University of Shanghai for Science and Technology, 516 Jungong Road, Shanghai 200093, People's Republic of China.
authors.forename: Cuilian
authors.initials: C
authors.lastname: Tao
authors.affiliation: School of Materials Science and Engineering, University of Shanghai for Science and Technology, 516 Jungong Road, Shanghai 200093, People's Republic of China. Electronic address: zjf2412@163.com.
authors.forename: Yufang
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: Department of Orthopedics, Shanghai Sixth People's Hospital, Shanghai Jiao Tong University, 600 Yishan Road, Shanghai 200233, People's Republic of China. Electronic address: shzhangchangqing@163.com.
authors.forename: Changqing
authors.initials: C
authors.lastname: Zhang
mesh_terms: D016475:3T3 Cells; D000818:Animals; D001672:Biocompatible Materials; D005898:Glass; D051379:Mice; D008855:Microscopy, Electron, Scanning; D010006:Osteoblasts / Q000166:cytology; D051381:Rats; D012886:Skull / Q000002:abnormalities*; D013324:Strontium / Q000737:chemistry*; D054457:Tissue Scaffolds; D055114:X-Ray Microtomography
chemical_list: D001672:Biocompatible Materials; D013324:Strontium
keywords: 3-D printing; Bone regeneration; In vivo; Mesoporous bioactive glass; Strontium