title: Evaluation of porcine xenograft in collateral ligament reconstruction in beagle dogs.
abstract: This study evaluated the effectiveness of irradiated porcine tendon xenografts for lateral collateral ligament (LCL) reconstruction. Twenty healthy adult beagle dogs underwent LCL reconstruction using irradiated porcine tendons treated with poly-gamma-glutamic acid. Serological and histological assessments were performed to evaluate host immunological response at 3 and 12 months after surgery. The healing and functional integrity of the LCL reconstructions were assessed by mechanical testing and gait analysis. Histological assessment of the porcine xenografts showed gradual host cellular infiltration and graft collagen remodeling during the healing process. Porcine xenografts showed angiogenesis and no signs of inflammatory reaction. Additionally, biomechanical and gait evaluations supported graft functional integration with no differences between normal and porcine xenograft reconstruction at 12 months after surgery. Irradiated porcine xenografts showed greater cellular responses and healing properties in short- and long-term evaluations. Irradiated porcine tendons appear to be useful as xenografts for the reconstruction of damaged ligaments.
authors.affiliation: Department of Laboratory Animal Medicine, College of Veterinary Medicine, Chonbuk National University, Jeonju, Republic of Korea.
authors.forename: Ji-Young
authors.initials: JY
authors.lastname: Na
authors.affiliation: Department of Laboratory Animal Medicine, College of Veterinary Medicine, Chonbuk National University, Jeonju, Republic of Korea.
authors.forename: Kibbeum
authors.initials: K
authors.lastname: Song
authors.affiliation: Department of Laboratory Animal Medicine, College of Veterinary Medicine, Chonbuk National University, Jeonju, Republic of Korea.
authors.forename: Sokho
authors.initials: S
authors.lastname: Kim
authors.affiliation: Department of Surgery, College of Veterinary Medicine, Chungnam National University, Daejeon, Republic of Korea.
authors.forename: Hae-Beom
authors.initials: HB
authors.lastname: Lee
authors.affiliation: Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeongeup, Republic of Korea.
authors.forename: Jae-Kyung
authors.initials: JK
authors.lastname: Kim
authors.affiliation: Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeongeup, Republic of Korea.
authors.forename: Jae-Hun
authors.initials: JH
authors.lastname: Kim
authors.affiliation: Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeongeup, Republic of Korea.
authors.forename: Ju-Woon
authors.initials: JW
authors.lastname: Lee
authors.affiliation: Department of Laboratory Animal Medicine, College of Veterinary Medicine, Chonbuk National University, Jeonju, Republic of Korea. Electronic address: jkwon@jbnu.ac.kr.
authors.forename: Jungkee
authors.initials: J
authors.lastname: Kwon
mesh_terms: D000818:Animals; D001696:Biomechanical Phenomena; D017885:Collateral Ligaments / Q000601:surgery*; D004285:Dogs; D005684:Gait / Q000502:physiology*; D005720:Gamma Rays; D064593:Heterografts / Q000033:anatomy & histology / Q000502:physiology* / Q000528:radiation effects; D019651:Plastic Surgery Procedures / Q000662:veterinary*; D013552:Swine; D013710:Tendons / Q000528:radiation effects / Q000637:transplantation*; D014183:Transplantation, Heterologous / Q000662:veterinary*; D014945:Wound Healing / Q000502:physiology / Q000528:radiation effects
keywords: Irradiation; Lateral collateral ligament; Porcine; Reconstruction; Xenograft