title: Pilot scale conversion of wheat straw to ethanol via simultaneous saccharification and fermentation.
abstract: The production of ethanol from wheat straw (WS) by dilute acid pretreatment, bioabatement of fermentation inhibitors by a fungal strain, and simultaneous saccharification and fermentation (SSF) of the bio-abated WS to ethanol using an ethanologenic recombinant bacterium was studied at a pilot scale without sterilization. WS (124.2g/L) was pretreated with dilute H2SO4 in two parallel tube reactors at 160°C. The inhibitors were bio-abated by growing the fungus aerobically. The maximum ethanol produced by SSF of the bio-abated WS by the recombinant Escherichia coli FBR5 at pH 6.0 and 35°C was 36.0g/L in 83h with a productivity of 0.43gL(-1)h(-1). This value corresponds to an ethanol yield of 0.29g/g of WS which is 86% of the theoretical ethanol yield from WS. This is the first report on the production of ethanol by the recombinant bacterium from a lignocellulosic biomass at a pilot scale.
authors.affiliation: Bioenergy Research Unit, National Center for Agricultural Utilization Research, Agricultural Research Service, U.S. Department of Agriculture, Peoria, IL 61604, USA. Electronic address: Badal.Saha@ars.usda.gov.
authors.forename: Badal C
authors.initials: BC
authors.lastname: Saha
authors.affiliation: Bioenergy Research Unit, National Center for Agricultural Utilization Research, Agricultural Research Service, U.S. Department of Agriculture, Peoria, IL 61604, USA.
authors.forename: Nancy N
authors.initials: NN
authors.lastname: Nichols
authors.affiliation: Bioenergy Research Unit, National Center for Agricultural Utilization Research, Agricultural Research Service, U.S. Department of Agriculture, Peoria, IL 61604, USA.
authors.forename: Nasib
authors.initials: N
authors.lastname: Qureshi
authors.affiliation: Bioenergy Research Unit, National Center for Agricultural Utilization Research, Agricultural Research Service, U.S. Department of Agriculture, Peoria, IL 61604, USA.
authors.forename: Gregory J
authors.initials: GJ
authors.lastname: Kennedy
authors.affiliation: Bioenergy Research Unit, National Center for Agricultural Utilization Research, Agricultural Research Service, U.S. Department of Agriculture, Peoria, IL 61604, USA.
authors.forename: Loren B
authors.initials: LB
authors.lastname: Iten
authors.affiliation: Bioenergy Research Unit, National Center for Agricultural Utilization Research, Agricultural Research Service, U.S. Department of Agriculture, Peoria, IL 61604, USA.
authors.forename: Michael A
authors.initials: MA
authors.lastname: Cotta
mesh_terms: D018533:Biomass; D019149:Bioreactors / Q000382:microbiology*; D004926:Escherichia coli; D000431:Ethanol / Q000138:chemical synthesis* / Q000737:chemistry; D005285:Fermentation; D010865:Pilot Projects; D014908:Triticum / Q000737:chemistry*
chemical_list: D000431:Ethanol
keywords: Ethanol; Ethanol from wheat straw; Recombinant ethanologenic Escherichia coli; Simultaneous saccharification and fermentation; Wheat straw