title: Study on the interaction characteristics of cefamandole with bovine serum albumin by spectroscopic technique.
abstract: The interaction of cefamandole with bovine serum albumin (BSA) was studied by fluorescence quenching in combination with UV-Vis spectroscopic method under near physiological conditions. The fluorescence quenching rate constants and binding constants for BSA-cefamandole system were determined at different temperatures. The fluorescence quenching of BSA by cefamandole is due to static quenching and energy transfer. The results of thermodynamic parameters, ΔH (-268.0 kJ mol(-1)), ΔS (-810.0 J mol(-1) K(-1)) and ΔG (-26.62 to -8.52 kJ mol(-1)), indicated that van der Waals interaction and hydrogen bonding played a major role for cefamandole-BSA association. The competitive experiments demonstrated that the primary binding site of cefamandole on BSA was located at site III in sub-domain IIIA of BSA. The distance between cefamandole and a tryptophane unit was estimated to be 1.18 nm based on the Förster resonance energy transfer theory. The binding constant (KA) of BSA-cefamandole at 298 K was 2.239×10(4) L mol(-1). Circular dichroism spectra, synchronous fluorescence and three-dimensional fluorescence studies showed that the presence of cefamandole could change the conformation of BSA during the binding process.
authors.affiliation: College of Chemistry and Environmental Science, Hebei University, Key Laboratory of Analytical Science and Technology of Hebei Province, Baoding 071002, PR China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Wang
authors.affiliation: College of Chemistry and Environmental Science, Hebei University, Key Laboratory of Analytical Science and Technology of Hebei Province, Baoding 071002, PR China.
authors.forename: Xuyang
authors.initials: X
authors.lastname: Liu
authors.affiliation: College of Chemistry and Environmental Science, Hebei University, Key Laboratory of Analytical Science and Technology of Hebei Province, Baoding 071002, PR China.
authors.forename: Ming
authors.initials: M
authors.lastname: Su
authors.affiliation: College of Chemistry and Environmental Science, Hebei University, Key Laboratory of Analytical Science and Technology of Hebei Province, Baoding 071002, PR China.
authors.forename: Zhihong
authors.initials: Z
authors.lastname: Shi
authors.affiliation: College of Chemistry and Environmental Science, Hebei University, Key Laboratory of Analytical Science and Technology of Hebei Province, Baoding 071002, PR China. Electronic address: hanwen@hbu.cn.
authors.forename: Hanwen
authors.initials: H
authors.lastname: Sun
mesh_terms: D000818:Animals; D001665:Binding Sites; D002417:Cattle; D002435:Cefamandole / Q000378:metabolism*; D002942:Circular Dichroism; D004735:Energy Transfer; D007700:Kinetics; D011485:Protein Binding; D012044:Regression Analysis; D012710:Serum Albumin, Bovine / Q000378:metabolism*; D013050:Spectrometry, Fluorescence; D013696:Temperature
chemical_list: D012710:Serum Albumin, Bovine; D002435:Cefamandole
keywords: Binding constant; Bovine serum albumin; Cefamandole; Circular dichroism; Fluorescence quenching; Three-dimensional fluorescence