title: Highly sensitive reduced graphene oxide microelectrode array sensor.
abstract: Reduced graphene oxide (rGO) has been fabricated into a microelectrode array (MEA) using a modified nanoimprint lithography (NIL) technique. Through a modified NIL process, the rGO MEA was fabricated by a self-alignment of conducting Indium Tin Oxide (ITO) and rGO layer without etching of the rGO layer. The rGO MEA consists of an array of 10μm circular disks and microelectrode signature has been found at a pitch spacing of 60μm. The rGO MEA shows a sensitivity of 1.91nAμm(-1) to dopamine (DA) without the use of mediators or functionalization of the reduced graphene oxide (rGO) active layer. The performance of rGO MEA remains stable when tested under highly resistive media using a continuous flow set up, as well as when subjecting it to mechanical stress. The successful demonstration of NIL for fabricating rGO microelectrodes on flexible substrate presents a route for the large scale fabrication of highly sensitive, flexible and thin biosensing platform.
authors.affiliation: Institute of Materials Research and Engineering, A⁎STAR (Agency for Science, Technology and Research), 3 Research Link, Singapore 117602, Singapore; Graphene Research Centre, Department of Chemistry, National University of Singapore, 3 Science Drive 3, Singapore 117543, Singapore.
authors.forename: Andrew M H
authors.initials: AM
authors.lastname: Ng
authors.affiliation: Graphene Research Centre, Department of Chemistry, National University of Singapore, 3 Science Drive 3, Singapore 117543, Singapore; NUS Graduate School for Integrative Sciences and Engineering, National University of Singapore, Singapore 117576, Singapore; Department of Biomedical Engineering, National University of Singapore, Singapore 117576, Singapore.
authors.lastname: Kenry
authors.affiliation: Graphene Research Centre, Department of Chemistry, National University of Singapore, 3 Science Drive 3, Singapore 117543, Singapore; Department of Biomedical Engineering, National University of Singapore, Singapore 117576, Singapore; Mechanobiology Institute, National University of Singapore, Singapore 117411, Singapore.
authors.forename: Chwee
authors.initials: C
authors.lastname: Teck Lim
authors.affiliation: Institute of Materials Research and Engineering, A⁎STAR (Agency for Science, Technology and Research), 3 Research Link, Singapore 117602, Singapore; Engineering Product Development, Singapore University of Technology and Design, 20, Dover Drive, Singapore 138682, Singapore. Electronic address: hongyee_low@sutd.edu.sg.
authors.forename: Hong Yee
authors.initials: HY
authors.lastname: Low
authors.affiliation: Graphene Research Centre, Department of Chemistry, National University of Singapore, 3 Science Drive 3, Singapore 117543, Singapore. Electronic address: chmlohkp@nus.edu.sg.
authors.forename: Kian Ping
authors.initials: KP
authors.lastname: Loh
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation*; D004298:Dopamine / Q000032:analysis; D015259:Dopamine Agents / Q000032:analysis; D055664:Electrochemical Techniques / Q000295:instrumentation*; D004867:Equipment Design; D006108:Graphite / Q000737:chemistry*; D056656:Lab-On-A-Chip Devices; D008839:Microelectrodes; D010084:Oxidation-Reduction; D010087:Oxides / Q000737:chemistry*; D017971:Tin Compounds / Q000737:chemistry
chemical_list: D015259:Dopamine Agents; D010087:Oxides; D017971:Tin Compounds; C109984:indium tin oxide; D006108:Graphite; D004298:Dopamine
keywords: Biosensor; Graphene; Microelectrode array; Nanoimprinting