title: Molecular characterization of macrolide resistance of a Mycoplasma pneumoniae strain that developed during therapy of a patient with pneumonia.
abstract: The development of macrolide resistance that occurred during 3 days of therapy with azithromycin to treat Mycoplasma pneumoniae pneumonia in a paediatric patient is reported. After extended molecular characterization of strains, the parallel occurrence of clones showing the non-mutated wild-type 23S rRNA sequence as well as mutations A2063G and A2064G, which are both responsible for phenotypic resistance, was confirmed for the first time.
authors.affiliation: TU Dresden, Medizinische Fakultät Carl Gustav Carus, Institut für Medizinische Mikrobiologie und Hygiene, Fetscherstrasse 74, 01307 Dresden, Germany. Electronic address: roger.dumke@tu-dresden.de.
authors.forename: Roger
authors.initials: R
authors.lastname: Dumke
authors.affiliation: Klinik für Kinder- und Jugendmedizin, Carl-Thiem-Klinikum gGmbH, Cottbus, Germany.
authors.forename: Simone
authors.initials: S
authors.lastname: Stolz
authors.affiliation: TU Dresden, Medizinische Fakultät Carl Gustav Carus, Institut für Medizinische Mikrobiologie und Hygiene, Fetscherstrasse 74, 01307 Dresden, Germany.
authors.forename: Enno
authors.initials: E
authors.lastname: Jacobs
authors.affiliation: Institut für Mikrobiologie und Krankenhaushygiene, Carl-Thiem-Klinikum gGmbH, Cottbus, Germany.
authors.forename: Thomas
authors.initials: T
authors.lastname: Juretzek
mesh_terms: D000293:Adolescent; D000900:Anti-Bacterial Agents / Q000627:therapeutic use*; D017963:Azithromycin / Q000627:therapeutic use*; D024881:Drug Resistance, Bacterial / Q000235:genetics; D006801:Humans; D008297:Male; D009154:Mutation; D009177:Mycoplasma pneumoniae / Q000187:drug effects* / Q000235:genetics; D011019:Pneumonia, Mycoplasma / Q000175:diagnosis / Q000188:drug therapy* / Q000382:microbiology; D012338:RNA, Ribosomal, 23S / Q000235:genetics
chemical_list: D000900:Anti-Bacterial Agents; D012338:RNA, Ribosomal, 23S; D017963:Azithromycin
keywords: Community-acquired pneumonia; Mycoplasma pneumoniae; Typing Macrolide resistance