title: Increased percentage of IFN-γ producing CD56+CD3+ cells in active tuberculosis patients upon CFP-10 stimulation of peripheral mononuclear cells.
abstract: Aiming to identify a possible biomarker that distinguishes immune cellular response of active tuberculosis from latent infection. Peripheral blood mononuclear cells (PBMCs) of pulmonary tuberculosis patients (PTB), tuberculin positive household contacts (TST(+) HHC), and tuberculin negative non-household contacts (TST− Non HHC) were stimulated with PPD or CFP-10 and the percentage of CD69(+) cells, proliferating precursor and IFN-γ producing CD4(+), CD8(+), CD56(+)CD3(−) and CD56(+)CD3(+) cells were compared. IL-2, IL-12p70, IL-15, IL-18 and IL-10 were measured in culture supernatants. PTB and TST+ HHC presented higher percentages of CD69(+) cells, IFN-γ(+) and proliferating precursors in all subpopulations studied and higher IL-12p70 levels than TST- Non HHC. The increased percentage of IFN-γ producing CD56(+)CD3(+) cells in response to CFP-10 in PTB, compared with TST− Non HHC and the ratios between the percentage of CD56(+)CD3(+) cells/CD56(+)CD3(−) and CD8(+) cells producing IFN-γ suggest that these parameters may distinguish active TB from latently infected individuals.
authors.forename: Victoria E
authors.initials: VE
authors.lastname: Niño
authors.forename: Luis F
authors.initials: LF
authors.lastname: García
authors.forename: Mauricio
authors.initials: M
authors.lastname: Rojas
authors.forename: Víctor
authors.initials: V
authors.lastname: Campo
authors.forename: Gloria
authors.initials: G
authors.lastname: Ávila
authors.forename: Julio Cesar
authors.initials: JC
authors.lastname: Klínger
authors.forename: Blanca L
authors.initials: BL
authors.lastname: Ortiz
authors.forename: María Lilia
authors.initials: ML
authors.lastname: Díaz
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001426:Bacterial Proteins / Q000276:immunology*; D017252:CD3 Complex / Q000097:blood; D019002:CD56 Antigen / Q000097:blood; D049109:Cell Proliferation; D002478:Cells, Cultured; D016207:Cytokines / Q000096:biosynthesis; D005260:Female; D006801:Humans; D007371:Interferon-gamma / Q000096:biosynthesis*; D007694:Killer Cells, Natural / Q000276:immunology*; D007963:Leukocytes, Mononuclear / Q000276:immunology; D008297:Male; D008875:Middle Aged; D016176:T-Lymphocyte Subsets / Q000276:immunology*; D014373:Tuberculin / Q000276:immunology; D014374:Tuberculin Test; D014397:Tuberculosis, Pulmonary / Q000276:immunology*; D055815:Young Adult
chemical_list: D001426:Bacterial Proteins; D017252:CD3 Complex; D019002:CD56 Antigen; C117940:CFP-10 protein, Mycobacterium tuberculosis; D016207:Cytokines; C522918:NCAM1 protein, human; D014373:Tuberculin; D007371:Interferon-gamma