title: Paclitaxel/epigallocatechin gallate coloaded liposome: a synergistic delivery to control the invasiveness of MDA-MB-231 breast cancer cells.
abstract: Matrix metalloproteinases (MMPs) have been investigated as a potential target for treating invasive breast cancers. The chemotherapy for breast cancer is often prescribed as a combination of drugs. The present study investigates a novel strategy of combining a MMP inhibitor, Epigallocatechin gallate (EGCG), along with an anticancer drug, Paclitaxel (PTX), in the form of a liposomal co-delivery system. The developed PTX/EGCG co-loaded liposomes showed an entrapment of 77.11±2.30% and 59.11±3.51% for PTX and EGCG, respectively. The in vitro efficacy of the liposomes was assessed by their ability to promote apoptosis and curtail cell invasion. On all parameters, namely cytotoxicity and caspase-3 activity that are indicators of apoptosis, and MMP-2 and - 9 inhibition and invasion assays that are indicators of cell invasion, the PTX/EGCG co-loaded liposomes showed better results than each of the individual drug loaded liposomes. These findings demonstrate the synergistic outcome of PTX/EGCG combination and indicate the suitability of PTX/EGCG co-loaded liposomes for the treatment of invasive breast cancer.
authors.affiliation: Central Leather Research Institute, Council of Scientific and Industrial Research, Chennai, Tamil Nadu, India.
authors.forename: Satiesh Kumar
authors.initials: SK
authors.lastname: Ramadass
authors.affiliation: Alternative Energy and Nanotechnology Laboratory, Indian Institute of Technology Madras, Chennai, Tamil Nadu, India.
authors.forename: Niranjana Vaighya
authors.initials: NV
authors.lastname: Anantharaman
authors.affiliation: Entomology Research Institute, Loyola College, Chennai, Tamil Nadu, India.
authors.forename: Saravanan
authors.initials: S
authors.lastname: Subramanian
authors.affiliation: Department of Virology, King Institute of Preventive Medicine and Research, Chennai, Tamil Nadu, India.
authors.forename: Srinivasan
authors.initials: S
authors.lastname: Sivasubramanian
authors.affiliation: Central Leather Research Institute, Council of Scientific and Industrial Research, Chennai, Tamil Nadu, India. Electronic address: bmadhan76@yahoo.co.in.
authors.forename: Balaraman
authors.initials: B
authors.lastname: Madhan
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D053148:Caspase 3 / Q000235:genetics / Q000378:metabolism; D002392:Catechin / Q000031:analogs & derivatives* / Q000494:pharmacology; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D004339:Drug Compounding; D004357:Drug Synergism; D005260:Female; D015870:Gene Expression; D006801:Humans; D008081:Liposomes / Q000737:chemistry*; D042361:Mammary Glands, Human / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D020778:Matrix Metalloproteinase 2 / Q000235:genetics / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000235:genetics / Q000378:metabolism; D061965:Matrix Metalloproteinase Inhibitors / Q000494:pharmacology*; D017239:Paclitaxel / Q000494:pharmacology*
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D008081:Liposomes; D061965:Matrix Metalloproteinase Inhibitors; D002392:Catechin; C045651:epigallocatechin gallate; D053148:Caspase 3; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9; D017239:Paclitaxel
keywords: Breast cancer; Co-delivery; Epigallocatechin gallate; Liposomes; Paclitaxel