title: Comparison and evaluation of concurrent saccharification and anaerobic digestion of Napier grass after pretreatment by three microbial consortia.
abstract: Napier grass is potentially a viable feedstock for biofuel production. The present study investigated biological pretreatment of Napier grass by three microbial consortia followed by saccharification and anaerobic digestion. The pretreatment efficiencies of three microbial consortia were compared in terms of degradation ability, saccharide and biogas yield. The lignocellulose loss rates of Napier grass varied largely. The biomass pretreated by the consortium WSD-5 gave 43.4% and 66.2% total sugar yield under low and moderate loadings of commercial enzyme mixtures, while the highest yield was 83.2% pretreated by the consortium MC1 under a high enzyme loading. The maximum methane yield of pretreated samples by the consortia MC1, WSD-5 and XDC-2 were 259, 279, 247ml/g VS, respectively, which were 1.39, 1.49 and 1.32times greater than the values of the untreated controls. This study showed that pretreatments by MC1, WSD-5 and XDC-2 were capable of significantly enhancing both the saccharide and methane yields from Napier grass.
authors.affiliation: Center of Biomass Engineering, College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China; Department of Molecular Biosciences and Bioengineering, University of Hawaii at Manoa, Honolulu, HI 96822, USA.
authors.forename: Boting
authors.initials: B
authors.lastname: Wen
authors.affiliation: Center of Biomass Engineering, College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China.
authors.forename: Xufeng
authors.initials: X
authors.lastname: Yuan
authors.affiliation: Department of Molecular Biosciences and Bioengineering, University of Hawaii at Manoa, Honolulu, HI 96822, USA.
authors.forename: Qing X
authors.initials: QX
authors.lastname: Li
authors.affiliation: Center of Biomass Engineering, College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China.
authors.forename: Jingjing
authors.initials: J
authors.lastname: Liu
authors.affiliation: Center of Biomass Engineering, College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China.
authors.forename: Jiwei
authors.initials: J
authors.lastname: Ren
authors.affiliation: Center of Biomass Engineering, College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China. Electronic address: wxiaofen@cau.edu.cn.
authors.forename: Xiaofen
authors.initials: X
authors.lastname: Wang
authors.affiliation: Center of Biomass Engineering, College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China. Electronic address: acuizj@cau.edu.cn.
authors.forename: Zongjun
authors.initials: Z
authors.lastname: Cui
mesh_terms: D056804:Biofuels / Q000032:analysis; D018533:Biomass; D050260:Carbohydrate Metabolism; D008031:Lignin / Q000378:metabolism; D008697:Methane / Q000378:metabolism; D059013:Microbial Consortia; D006109:Poaceae / Q000737:chemistry*; D014866:Waste Products
chemical_list: D056804:Biofuels; D014866:Waste Products; C036909:lignocellulose; D008031:Lignin; D008697:Methane
keywords: Anaerobic digestion; Enzymatic hydrolysis; Microbial consortium; Pretreatment