title: Complete annotated genome sequence of Mycobacterium tuberculosis (Zopf) Lehmann and Neumann (ATCC35812) (Kurono).
abstract: We report the completely annotated genome sequence of Mycobacterium tuberculosis (Zopf) Lehmann and Neumann (ATCC35812) (Kurono), which is a used for virulence and/or immunization studies. The complete genome sequence of M. tuberculosis Kurono was determined with a length of 4,415,078 bp and a G+C content of 65.60%. The chromosome was shown to contain a total of 4,340 protein-coding genes, 53 tRNA genes, one transfer messenger RNA for all amino acids, and 1 rrn operon. Lineage analysis based on large sequence polymorphisms indicated that M. tuberculosis Kurono belongs to the Euro-American lineage (lineage 4). Phylogenetic analysis using whole genome sequences of M. tuberculosis Kurono in addition to 22 M. tuberculosis complex strains indicated that H37Rv is the closest relative of Kurono based on the results of phylogenetic analysis. These findings provide a basis for research using M. tuberculosis Kurono, especially in animal models.
authors.affiliation: Department of Infectious Diseases, Research Institute, National Center for Global Health and Medicine, 1-21-1 Toyama, Shinjuku, Tokyo, 162-8655, Japan; Pathogenic Microbe Laboratory, Research Institute, National Center for Global Health and Medicine, 1-21-1 Toyama, Shinjuku, Tokyo, 162-8655, Japan. Electronic address: takiyam@ri.ncgm.go.jp.
authors.forename: Tohru
authors.initials: T
authors.lastname: Miyoshi-Akiyama
authors.affiliation: Okinawa Life Science Research Center, 5-8 Suzaki, Uruma, Okinawa, 904-2234, Japan.
authors.forename: Kazuhito
authors.initials: K
authors.lastname: Satou
authors.affiliation: Department of Infectious Diseases, Research Institute, National Center for Global Health and Medicine, 1-21-1 Toyama, Shinjuku, Tokyo, 162-8655, Japan.
authors.forename: Masako
authors.initials: M
authors.lastname: Kato
authors.affiliation: Okinawa Life Science Research Center, 5-8 Suzaki, Uruma, Okinawa, 904-2234, Japan.
authors.forename: Akino
authors.initials: A
authors.lastname: Shiroma
authors.affiliation: Department of Infectious Diseases, Research Institute, National Center for Global Health and Medicine, 1-21-1 Toyama, Shinjuku, Tokyo, 162-8655, Japan.
authors.forename: Kazunori
authors.initials: K
authors.lastname: Matsumura
authors.affiliation: Okinawa Life Science Research Center, 5-8 Suzaki, Uruma, Okinawa, 904-2234, Japan.
authors.forename: Hinako
authors.initials: H
authors.lastname: Tamotsu
authors.affiliation: Department of Infectious Diseases, Research Institute, National Center for Global Health and Medicine, 1-21-1 Toyama, Shinjuku, Tokyo, 162-8655, Japan.
authors.forename: Hiroki
authors.initials: H
authors.lastname: Iwai
authors.affiliation: Okinawa Life Science Research Center, 5-8 Suzaki, Uruma, Okinawa, 904-2234, Japan.
authors.forename: Kuniko
authors.initials: K
authors.lastname: Teruya
authors.affiliation: Tochigi Prefectural Institute of Public Health and Environmental Science, 2145-13 Shimookamoto, Kawachi-machi, Tochigi, 329-1196, Japan.
authors.forename: Keiji
authors.initials: K
authors.lastname: Funatogawa
authors.affiliation: Okinawa Life Science Research Center, 5-8 Suzaki, Uruma, Okinawa, 904-2234, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Hirano
authors.affiliation: Department of Infectious Diseases, Research Institute, National Center for Global Health and Medicine, 1-21-1 Toyama, Shinjuku, Tokyo, 162-8655, Japan.
authors.forename: Teruo
authors.initials: T
authors.lastname: Kirikae
mesh_terms: D005798:Genes, Bacterial / Q000235:genetics; D016680:Genome, Bacterial / Q000235:genetics*; D006867:Hydrolases / Q000235:genetics; D009169:Mycobacterium tuberculosis / Q000235:genetics*; D010802:Phylogeny; D011110:Polymorphism, Genetic; D054852:Small Molecule Libraries
chemical_list: D054852:Small Molecule Libraries; D006867:Hydrolases; C527647:HsaD protein, Mycobacterium tuberculosis
keywords: Complete genome; Kurono; PE-PPE; PacBio sequencer; Phyrogenetic tree