title: Gene dynamics of core transcription factors for pluripotency in embryonic stem cells.
abstract: Embryonic stem cell (ESC) pluripotency is maintained by core transcription factors (TFs). Although the expression of these TFs is well documented, their expression dynamics is poorly evaluated. Here, we visualized the dynamics of Nanog and Oct3/4 expression in ESC using fluorescent reporters and found that expression of these TFs change dramatically during culture.
authors.affiliation: Graduate School of Frontier Bioscience, Osaka University, 1-3 Yamadaoka, Suita, Osaka 565-0871, Japan.
authors.forename: Brit G
authors.initials: BG
authors.lastname: David
authors.affiliation: Quantitative Biology Center (QBiC), Riken, Suita, Osaka 565-0874, Japan.
authors.forename: Kazuko
authors.initials: K
authors.lastname: Okamoto
authors.affiliation: Graduate School of Frontier Bioscience, Osaka University, 1-3 Yamadaoka, Suita, Osaka 565-0871, Japan.
authors.forename: Taishi
authors.initials: T
authors.lastname: Kakizuka
authors.affiliation: Quantitative Biology Center (QBiC), Riken, Suita, Osaka 565-0874, Japan.
authors.forename: Taro
authors.initials: T
authors.lastname: Ichimura
authors.affiliation: Graduate School of Frontier Bioscience, Osaka University, 1-3 Yamadaoka, Suita, Osaka 565-0871, Japan; Quantitative Biology Center (QBiC), Riken, Suita, Osaka 565-0874, Japan.
authors.forename: Tomonobu M
authors.initials: TM
authors.lastname: Watanabe
authors.affiliation: Graduate School of Frontier Bioscience, Osaka University, 1-3 Yamadaoka, Suita, Osaka 565-0871, Japan; Quantitative Biology Center (QBiC), Riken, Suita, Osaka 565-0874, Japan; WPI, Immunology Frontier Research Center (IFReC), Osaka University, Suita, Osaka 565-0871, Japan. Electronic address: hideaki.fujita@riken.jp.
authors.forename: Hideaki
authors.initials: H
authors.lastname: Fujita
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques; D002478:Cells, Cultured; D053595:Embryonic Stem Cells / Q000166:cytology / Q000378:metabolism*; D017930:Genes, Reporter / Q000235:genetics; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism; D051379:Mice; D000071317:Nanog Homeobox Protein; D050814:Octamer Transcription Factor-3 / Q000235:genetics / Q000378:metabolism; D039904:Pluripotent Stem Cells / Q000166:cytology / Q000378:metabolism*; D055748:SOXB1 Transcription Factors / Q000235:genetics / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism
chemical_list: D018398:Homeodomain Proteins; D000071317:Nanog Homeobox Protein; C475540:Nanog protein, mouse; D050814:Octamer Transcription Factor-3; D055748:SOXB1 Transcription Factors; C527783:Sox2 protein, mouse; D014157:Transcription Factors
keywords: Embryonic stem cell; Imaging; Nanog; Oct3/4; Sox2