title: Low-level laser therapy attenuates LPS-induced rats mastitis by inhibiting polymorphonuclear neutrophil adhesion.
abstract: The aim of this study was to investigate the effects of low-level laser therapy (LLLT) on a rat model of lipopolysaccharide (LPS)-induced mastitis and its underlying molecular mechanisms. The rat model of mastitis was induced by inoculation of LPS through the canals of the mammary gland. The results showed that LPS-induced secretion of IL-1β and IL-8 significantly decreased after LLLT (650 nm, 2.5 mW, 30 mW/cm(2)). LLLT also inhibited intercellular adhesion molecule-1 (ICAM-1) expression and attenuated the LPS-induced decrease of the expression of CD62L and increase of the expression of CD11b. Moreover, LLLT also suppressed LPS-induced polymorphonuclear neutrophils (PMNs) entering the alveoli of the mammary gland. The number of PMNs in the mammary alveolus and the myeloperoxidase (MPO) activity were decreased after LLLT. These results suggested that LLLT therapy is beneficial in decreasing the somatic cell count and improving milk nutritional quality in cows with an intramammary infection.
authors.affiliation: College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing 163319, P.R. China.
authors.forename: Yueqiang
authors.initials: Y
authors.lastname: Wang
authors.forename: Xianjing
authors.initials: X
authors.lastname: He
authors.forename: Dandan
authors.initials: D
authors.lastname: Hao
authors.forename: Debin
authors.initials: D
authors.lastname: Yu
authors.forename: Jianbin
authors.initials: J
authors.lastname: Liang
authors.forename: Yanpeng
authors.initials: Y
authors.lastname: Qu
authors.forename: Dongbo
authors.initials: D
authors.lastname: Sun
authors.forename: Bin
authors.initials: B
authors.lastname: Yang
authors.forename: Keli
authors.initials: K
authors.lastname: Yang
authors.forename: Rui
authors.initials: R
authors.lastname: Wu
authors.forename: Jianfa
authors.initials: J
authors.lastname: Wang
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D039481:CD11b Antigen / Q000378:metabolism; D017931:DNA Primers / Q000235:genetics; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005434:Flow Cytometry; D005786:Gene Expression Regulation / Q000187:drug effects; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism; D053583:Interleukin-1beta / Q000378:metabolism; D016209:Interleukin-8 / Q000378:metabolism; D019041:L-Selectin / Q000378:metabolism; D008070:Lipopolysaccharides / Q000633:toxicity; D028022:Low-Level Light Therapy / Q000379:methods*; D008413:Mastitis / Q000139:chemically induced / Q000628:therapy*; D009504:Neutrophils / Q000378:metabolism; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar
chemical_list: D039481:CD11b Antigen; D017931:DNA Primers; D053583:Interleukin-1beta; D016209:Interleukin-8; D008070:Lipopolysaccharides; D019408:Platelet Endothelial Cell Adhesion Molecule-1; D018799:Intercellular Adhesion Molecule-1; D019041:L-Selectin