title: Generation of functionalized polymer nanolayer on implant surface via initiated chemical vapor deposition (iCVD).
abstract: Initiated chemical vapor deposition (iCVD) was utilized to generate a 200nm thick, uniform, functionalized polymer nanolayer comprised of glycidyl methacrylate (GMA) on the surface of titanium implants as a means to improve cellular attachment. Dot-patterned GMA-coated specimens were prepared as well as fully coated specimens. In vitro cellular responses, including cell morphology, protein adsorption, cell proliferation assays, alkaline phosphate activity (ALP) assays, and calcium deposition assays were studied using adipose derived stem cells. The mechanical stability of the thin film was investigated by XPS and FE-SEM analysis of the GMA-coated implant after implantation to an extracted bone from a pig. The GMA-coated specimens displayed increased protein adsorption, higher alkaline phosphatase activities, and higher calcium deposition as compared to control sample with no cytotoxicity. Additionally, no defect was observed in the test of mechanical stability. Notably, dot-patterned GMA-coated samples displayed higher alkaline phosphatase activities than others. Functionalized polymer nanolayer deposition via iCVD is a flexible and robust technique capable of mass production of biocompatible layers. These properties make this technique very suitable for implant applications in a variety of ways.
authors.affiliation: Department of Maxillofacial Biomedical Engineering and Institute of Oral Biology, School of Dentistry, Kyung Hee University, Seoul 130-701, Republic of Korea.
authors.forename: Se Woong
authors.initials: SW
authors.lastname: Park
authors.affiliation: Department of Maxillofacial Biomedical Engineering and Institute of Oral Biology, School of Dentistry, Kyung Hee University, Seoul 130-701, Republic of Korea.
authors.forename: Donghyun
authors.initials: D
authors.lastname: Lee
authors.affiliation: Department of Chemical and Biomolecular Engineering and KI for NanoCentury, Korea Advanced Institute of Science and Technology, Daejeon 305-701, Republic of Korea.
authors.forename: Hak Rae
authors.initials: HR
authors.lastname: Lee
authors.affiliation: Department of Maxillofacial Biomedical Engineering and Institute of Oral Biology, School of Dentistry, Kyung Hee University, Seoul 130-701, Republic of Korea.
authors.forename: Ho-Jin
authors.initials: HJ
authors.lastname: Moon
authors.affiliation: Department of Chemical and Biomolecular Engineering and KI for NanoCentury, Korea Advanced Institute of Science and Technology, Daejeon 305-701, Republic of Korea.
authors.forename: Bo Ra
authors.initials: BR
authors.lastname: Lee
authors.affiliation: Department of Maxillofacial Biomedical Engineering and Institute of Oral Biology, School of Dentistry, Kyung Hee University, Seoul 130-701, Republic of Korea.
authors.forename: Wan-Kyu
authors.initials: WK
authors.lastname: Ko
authors.affiliation: Department of Maxillofacial Biomedical Engineering and Institute of Oral Biology, School of Dentistry, Kyung Hee University, Seoul 130-701, Republic of Korea.
authors.forename: Su-Jin
authors.initials: SJ
authors.lastname: Song
authors.affiliation: Department of Maxillofacial Biomedical Engineering and Institute of Oral Biology, School of Dentistry, Kyung Hee University, Seoul 130-701, Republic of Korea.
authors.forename: Sang Jin
authors.initials: SJ
authors.lastname: Lee
authors.affiliation: Department of Chemistry and Institute of Biological Interfaces, Sogang University, Seoul 121-742, Republic of Korea.
authors.forename: Kwanwoo
authors.initials: K
authors.lastname: Shin
authors.affiliation: Department of Chemistry and Institute of Biological Interfaces, Sogang University, Seoul 121-742, Republic of Korea.
authors.forename: Wonhyeong
authors.initials: W
authors.lastname: Jang
authors.affiliation: Department of Conservative Dentistry, School of Dentistry, Kyung Hee University, Seoul 130-701, Republic of Korea.
authors.forename: Jin-Kyu
authors.initials: JK
authors.lastname: Yi
authors.affiliation: Department of Chemical and Biomolecular Engineering and KI for NanoCentury, Korea Advanced Institute of Science and Technology, Daejeon 305-701, Republic of Korea. Electronic address: sgim@kaist.ac.kr.
authors.forename: Sung Gap
authors.initials: SG
authors.lastname: Im
authors.affiliation: Department of Maxillofacial Biomedical Engineering and Institute of Oral Biology, School of Dentistry, Kyung Hee University, Seoul 130-701, Republic of Korea. Electronic address: kwoni@khu.ac.kr.
authors.forename: Il Keun
authors.initials: IK
authors.lastname: Kwon
mesh_terms: D000327:Adsorption; D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible / Q000737:chemistry* / Q000633:toxicity; D005740:Gases; D006801:Humans; D008855:Microscopy, Electron, Scanning; D015394:Molecular Structure; D036103:Nanotechnology; D011108:Polymers / Q000737:chemistry* / Q000633:toxicity; D013234:Stem Cells; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry
chemical_list: D020099:Coated Materials, Biocompatible; D005740:Gases; D011108:Polymers; D014025:Titanium
keywords: Functional polymer; Implant; Initiated chemical vapor deposition; Surface treatment