title: Identification of Ppk26, a DEG/ENaC Channel Functioning with Ppk1 in a Mutually Dependent Manner to Guide Locomotion Behavior in Drosophila.
abstract: A major gap in our understanding of sensation is how a single sensory neuron can differentially respond to a multitude of different stimuli (polymodality), such as propio- or nocisensation. The prevailing hypothesis is that different stimuli are transduced through ion channels with diverse properties and subunit composition. In a screen for ion channel genes expressed in polymodal nociceptive neurons, we identified Ppk26, a member of the trimeric degenerin/epithelial sodium channel (DEG/ENaC) family, as being necessary for proper locomotion behavior in Drosophila larvae in a mutually dependent fashion with coexpressed Ppk1, another member of the same family. Mutants lacking Ppk1 and Ppk26 were defective in mechanical, but not thermal, nociception behavior. Mutants of Piezo, a channel involved in mechanical nociception in the same neurons, did not show a defect in locomotion, suggesting distinct molecular machinery for mediating locomotor feedback and mechanical nociception.
authors.affiliation: Neuroscience Graduate Program, University of California San Francisco, San Francisco, CA 94158, USA; Departments of Physiology, Biochemistry, and Biophysics, University of California San Francisco, Howard Hughes Medical Institute, San Francisco, CA 94158, USA.
authors.forename: David A
authors.initials: DA
authors.lastname: Gorczyca
authors.affiliation: Neuroscience Graduate Program, University of California San Francisco, San Francisco, CA 94158, USA.
authors.forename: Susan
authors.initials: S
authors.lastname: Younger
authors.affiliation: Neuroscience Graduate Program, University of California San Francisco, San Francisco, CA 94158, USA; Departments of Physiology, Biochemistry, and Biophysics, University of California San Francisco, Howard Hughes Medical Institute, San Francisco, CA 94158, USA.
authors.forename: Shan
authors.initials: S
authors.lastname: Meltzer
authors.affiliation: Neuroscience Graduate Program, University of California San Francisco, San Francisco, CA 94158, USA.
authors.forename: Sung Eun
authors.initials: SE
authors.lastname: Kim
authors.affiliation: Neuroscience Graduate Program, University of California San Francisco, San Francisco, CA 94158, USA.
authors.forename: Li
authors.initials: L
authors.lastname: Cheng
authors.affiliation: Neuroscience Graduate Program, University of California San Francisco, San Francisco, CA 94158, USA.
authors.forename: Wei
authors.initials: W
authors.lastname: Song
authors.affiliation: Neuroscience Graduate Program, University of California San Francisco, San Francisco, CA 94158, USA.
authors.forename: Hye Young
authors.initials: HY
authors.lastname: Lee
authors.affiliation: Neuroscience Graduate Program, University of California San Francisco, San Francisco, CA 94158, USA.
authors.forename: Lily Yeh
authors.initials: LY
authors.lastname: Jan
authors.affiliation: Neuroscience Graduate Program, University of California San Francisco, San Francisco, CA 94158, USA. Electronic address: yuhnung.jan@ucsf.edu.
authors.forename: Yuh Nung
authors.initials: YN
authors.lastname: Jan
mesh_terms: D000818:Animals; D001522:Behavior, Animal; D002462:Cell Membrane / Q000378:metabolism; D062626:Degenerin Sodium Channels / Q000378:metabolism*; D003712:Dendrites / Q000378:metabolism; D029721:Drosophila Proteins / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000502:physiology*; D053503:Epithelial Sodium Channels / Q000378:metabolism*; D008124:Locomotion; D009154:Mutation / Q000235:genetics; D059225:Nociception; D011485:Protein Binding; D021122:Protein Subunits / Q000378:metabolism; D015222:Sodium Channels / Q000378:metabolism*; D013696:Temperature
chemical_list: D062626:Degenerin Sodium Channels; D029721:Drosophila Proteins; D053503:Epithelial Sodium Channels; C000599464:PPK26 protein, Drosophila; D021122:Protein Subunits; D015222:Sodium Channels; C532965:ppk protein, Drosophila