title: Morphological evaluation of sperm from infertile men selected by magnetic activated cell sorting (MACS).
abstract: Electron microscopy analysis performed in five infertile human subjects after sperm selection by swim-up followed by magnetic activated cell sorting (MACS) demonstrated a decrease in the number of spermatozoa with characteristics compatible with cell death. However, no significant differences were found when the swim-up/MACS semen fraction was compared with swim-up fraction alone.
authors.affiliation: Departamento de Biologia Molecular - Instituto De Investigaciones Biológicas Clemente Estable (IIBCE), Avenida Italia 3318, CP 11600 Montevideo, Uruguay. Electronic address: giannicurti@gmail.com.
authors.forename: Gianni
authors.initials: G
authors.lastname: Curti
authors.affiliation: Laboratorio de Biología Molecular de la Reproducción - Departamento de Histología y Embriología, Facultad de Medicina, Gral. Flores 2125, 11800 Montevideo, Uruguay. Electronic address: fernandaskowronek@gmail.com.
authors.forename: Fernanda
authors.initials: F
authors.lastname: Skowronek
authors.affiliation: Centro de Reproducción Humana del Interior (CERHIN), Bvar. Artigas 1665/102, Montevideo, Uruguay. Electronic address: draritaverno@gmail.com.
authors.forename: Rita
authors.initials: R
authors.lastname: Vernochi
authors.affiliation: Centro de Reproducción Humana del Interior (CERHIN), Bvar. Artigas 1665/102, Montevideo, Uruguay. Electronic address: anucharod@gmail.com.
authors.forename: Ana Laura
authors.initials: AL
authors.lastname: Rodriguez-Buzzi
authors.affiliation: Centro de Reproducción Humana del Interior (CERHIN), Bvar. Artigas 1665/102, Montevideo, Uruguay. Electronic address: jcrbuzzi@gmail.com.
authors.forename: Juan Carlos
authors.initials: JC
authors.lastname: Rodriguez-Buzzi
authors.affiliation: Unidad de Microscopía Electrónica de Transmisión - Facultad de Ciencias, Iguá 4225, 11400 Montevideo, Uruguay. Electronic address: paragaby@gmail.com.
authors.forename: Gabriela
authors.initials: G
authors.lastname: Casanova
authors.affiliation: Laboratorio de Biología Molecular de la Reproducción - Departamento de Histología y Embriología, Facultad de Medicina, Gral. Flores 2125, 11800 Montevideo, Uruguay. Electronic address: rsapiro@fmed.edu.uy.
authors.forename: Rossana
authors.initials: R
authors.lastname: Sapiro
mesh_terms: D000328:Adult; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D007248:Infertility, Male / Q000473:pathology*; D008280:Magnetics / Q000379:methods*; D008297:Male; D008854:Microscopy, Electron; D013094:Spermatozoa / Q000648:ultrastructure*; D018709:Statistics, Nonparametric
keywords: Annexin V; Apoptotic sperm; DNA damage; Electron microscopy; Magnetic activated cell sorting