title: Simulating distal gut mucosal and luminal communities using packed-column biofilm reactors and an in vitro chemostat model.
abstract: In vivo studies of human mucosal gut microbiota are often limited to end-point analyses and confounded by bowel cleansing procedures. Therefore, we used biofilm reactors to incorporate a simulated mucosal environment into an in vitro gut chemostat model. Communities developed were complex, reproducible, distinct, and representative of in vivo communities.
authors.affiliation: Department of Molecular and Cellular Biology, University of Guelph, 50 Stone Road East, Guelph, Ontario N1G 2W1, Canada.
authors.forename: Julie A K
authors.initials: JA
authors.lastname: McDonald
authors.affiliation: Laboratory of Microbiology, Wageningen University, Wageningen, The Netherlands.
authors.forename: Susana
authors.initials: S
authors.lastname: Fuentes
authors.affiliation: Department of Molecular and Cellular Biology, University of Guelph, 50 Stone Road East, Guelph, Ontario N1G 2W1, Canada.
authors.forename: Kathleen
authors.initials: K
authors.lastname: Schroeter
authors.affiliation: Laboratory of Microbiology, Wageningen University, Wageningen, The Netherlands.
authors.forename: Ineke
authors.initials: I
authors.lastname: Heikamp-deJong
authors.affiliation: Department of Molecular and Cellular Biology, University of Guelph, 50 Stone Road East, Guelph, Ontario N1G 2W1, Canada.
authors.forename: Cezar M
authors.initials: CM
authors.lastname: Khursigara
authors.affiliation: Laboratory of Microbiology, Wageningen University, Wageningen, The Netherlands.
authors.forename: Willem M
authors.initials: WM
authors.lastname: de Vos
authors.affiliation: Department of Molecular and Cellular Biology, University of Guelph, 50 Stone Road East, Guelph, Ontario N1G 2W1, Canada. Electronic address: eav@uoguelph.ca.
authors.forename: Emma
authors.initials: E
authors.lastname: Allen-Vercoe
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001419:Bacteria / Q000145:classification / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification; D018407:Bacterial Physiological Phenomena / Q000187:drug effects; D018441:Biofilms / Q000187:drug effects*; D002981:Clindamycin / Q000494:pharmacology*; D041981:Gastrointestinal Tract / Q000382:microbiology; D006801:Humans; D007413:Intestinal Mucosa / Q000382:microbiology*; D064307:Microbiota / Q000187:drug effects*; D008954:Models, Biological; D010802:Phylogeny
chemical_list: D000900:Anti-Bacterial Agents; D002981:Clindamycin
keywords: Biofilm; Chemostat; DGGE; HITChip; Human gut microbiota; Microbial ecology