title: Identification and quantification of flavonoids and chromes in Baeckea frutescens by using HPLC coupled with diode-array detection and quadruple time-of-flight mass spectrometry.
abstract: This article marks the first report on high-performance liquid chromatography (HPLC) coupled with diode-array detection (DAD) and quadruple time-of-flight mass spectrometry (Q-TOF/MS) for the identification and quantification of main bioactive constituents in Baeckea frutescens. In total, 24 compounds were identified or tentatively characterised based on their retention behaviours, UV profiles and MS fragment information. Furthermore, a validated method with good linearity, sensitivity, precision, stability, repeatability and accuracy was successfully applied for simultaneous determination of five flavonoids and one chromone in different plant parts of B. frutescens collected at different harvest times, and their dynamic contents revealed the appropriate harvest times. The established HPLC-DAD-Q-TOF/MS using multi-bioactive markers was proved to be a validated strategy for the quality evaluation on both raw materials and related products of B. frutescens.
authors.affiliation: a Department of Pharmacognosy , College of Pharmacy, Zhengzhou University , No.100 of Science Road, Zhengzhou 450001 , P.R. China.
authors.forename: Bei-Xi
authors.initials: BX
authors.lastname: Jia
authors.forename: Qian-Qian
authors.initials: QQ
authors.lastname: Huangfu
authors.forename: Feng-Xiao
authors.initials: FX
authors.lastname: Ren
authors.forename: Lu
authors.initials: L
authors.lastname: Jia
authors.forename: Yan-Bing
authors.initials: YB
authors.lastname: Zhang
authors.forename: Hong-Min
authors.initials: HM
authors.lastname: Liu
authors.forename: Jie
authors.initials: J
authors.lastname: Yang
authors.forename: Qiang
authors.initials: Q
authors.lastname: Wang
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002867:Chromones / Q000032:analysis*; D005419:Flavonoids / Q000032:analysis*; D015394:Molecular Structure; D027822:Myrtaceae / Q000737:chemistry*; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
chemical_list: D002867:Chromones; D005419:Flavonoids
keywords: Baeckea frutescens; HPLC-DAD-Q-TOF/MS; chromones; flavonoids; identification; quantification