title: Therapeutic effect of activated carbon-induced constipation mice with Lactobacillus fermentum Suo on treatment.
abstract: The aim of this study was to investigate the effects of Lactobacillus fermentum Suo (LF-Suo) on activated carbon-induced constipation in ICR (Institute of Cancer Research) mice. ICR mice were orally administered with lactic acid bacteria for 9 days. Body weight, diet intake, drinking amount, defecation status, gastrointestinal transit and defecation time, and the serum levels of MTL (motilin), Gas (gastrin), ET (endothelin), SS (somatostatin), AChE (acetylcholinesterase), SP (substance P), VIP (vasoactive intestinal peptide) were used to evaluate the preventive effects of LF-Suo on constipation. Bisacodyl, a laxative drug, was used as a positive control. The normal, control, 100 mg/kg bisacodyl treatment, LB (Lactobacillus bulgaricus)-, LF-Suo (L)- and LF-Suo (H)-treated mice showed the time to the first black stool defecation at 90, 218, 117, 180, 155 and 137 min, respectively. By the oral administration of LB-, LF-Suo (L), LF-Suo (H) or bisacodyl (100 mg/kg), the gastrointestinal transit was reduced to 55.2%, 72.3%, 85.5% and 94.6%, respectively, of the transit in normal mice, respectively. In contrast to the control mice, the serum levels of MTL, Gas, ET, AChE, SP and VIP were significantly increased and the serum levels of SS were reduced in the mice treated with LF-Suo (p < 0.05). By the RT-PCR (reverse transcription-polymerase chain reaction) and western blot assays, LF-Suo increased the c-Kit, SCF (stem cell factor), GDNF (glial cell line-derived neurotrophic factor) and decreased TRPV1 (transient receptor potential vanilloid 1), NOS (nitric oxide synthase) expressions of small intestine tissue in mice. These results demonstrate that lactic acid bacteria has preventive effects on mouse constipation and LF-Suo demonstrated the best functional activity.
authors.affiliation: College of Food Science, Southwest University, Chongqing 400715, China. birget@swu.edu.cn.
authors.forename: Huayi
authors.initials: H
authors.lastname: Suo
authors.affiliation: Department of Biological and Chemical Engineering, Chongqing University of Education, Chongqing 400067, China. zhaoxin@pun.edu.
authors.forename: Xin
authors.initials: X
authors.lastname: Zhao
authors.affiliation: Department of Biological and Chemical Engineering, Chongqing University of Education, Chongqing 400067, China. qianyubaby@gmail.com.
authors.forename: Yu
authors.initials: Y
authors.lastname: Qian
authors.affiliation: Department of Biological and Chemical Engineering, Chongqing University of Education, Chongqing 400067, China. quajetlee@gmail.com.
authors.forename: Guijie
authors.initials: G
authors.lastname: Li
authors.affiliation: Science and Technology Administration, Chinese Academy of Agricultural Sciences, Biejing 100081, China. liuzhenhu@caas.cn.
authors.forename: Zhenhu
authors.initials: Z
authors.lastname: Liu
authors.affiliation: College of Food Science, Southwest University, Chongqing 400715, China. xiejie1024@swu.edu.cn.
authors.forename: Jie
authors.initials: J
authors.lastname: Xie
authors.affiliation: Institute of Qinghai-Tibetan Plateau, Southwest University for Nationalities, Chengdu 610041, China. lijian@swun.cn.
authors.forename: Jian
authors.initials: J
authors.lastname: Li
mesh_terms: D000818:Animals; D001835:Body Weight; D002606:Charcoal; D003248:Constipation / Q000235:genetics / Q000503:physiopathology / Q000628:therapy*; D003672:Defecation; D004032:Diet; D004195:Disease Models, Animal; D004327:Drinking Behavior; D005260:Female; D005772:Gastrointestinal Transit; D005786:Gene Expression Regulation; D051100:Glial Cell Line-Derived Neurotrophic Factor / Q000235:genetics / Q000378:metabolism; D057927:Hydrophobic and Hydrophilic Interactions; D007421:Intestine, Small / Q000473:pathology / Q000503:physiopathology; D052197:Limosilactobacillus fermentum / Q000502:physiology*; D051379:Mice; D008813:Mice, Inbred ICR; D019001:Nitric Oxide Synthase / Q000235:genetics / Q000378:metabolism; D019009:Proto-Oncogene Proteins c-kit / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D019089:Stem Cell Factor / Q000235:genetics / Q000378:metabolism; D050916:TRPV Cation Channels / Q000235:genetics / Q000378:metabolism; D013997:Time Factors
chemical_list: D051100:Glial Cell Line-Derived Neurotrophic Factor; D012333:RNA, Messenger; D019089:Stem Cell Factor; D050916:TRPV Cation Channels; C493921:TRPV1 protein, mouse; D002606:Charcoal; D019001:Nitric Oxide Synthase; D019009:Proto-Oncogene Proteins c-kit