title: Avidin-biotin capped mesoporous silica nanoparticles as an ion-responsive release system to determine lead(II).
abstract: We have developed DNAzyme-functionalized silica nanoparticles for the rapid, sensitive, and selective detection of lead ion (Pb(2+)). The specific binding between avidin and biotinylated DNAzymes was used to cap the pore of dye-trapped silica nanoparticles. In the presence of Pb(2+), DNAzymes were catalytically cleaved to uncap the pore, releasing the dye cargo with detectable enhancements of fluorescence signal. This method enables rapid (15 min) and sensitive (limit of detection=8.0 nM) detection. Moreover, the Pb(2+)-responsive behavior shows high selectivity with other metal ions. The superior properties of the as-designed DNAzyme-functionalized silica nanoparticles can be attributed to the large loading capacity and highly ordered pore structure of mesoporous silica nanoparticles as well as the catalytical cleaving of DNAzymes with Pb(2+). The recoveries obtained by standard Pb(II) addition to real samples-tap water, commercial mineral water, and lake water-were all from 98 to 101%. Our design serves as a new prototype for metal-ion sensing systems, and it also has promising potential for detection of various targets in stimulus-release systems.
authors.affiliation: Shandong Provincial Key Laboratory of Biochemical Analysis, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, Shandong, People's Republic of China.
authors.forename: Weiling
authors.initials: W
authors.lastname: Song
authors.affiliation: Shandong Provincial Key Laboratory of Biochemical Analysis, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, Shandong, People's Republic of China.
authors.forename: Jingyu
authors.initials: J
authors.lastname: Li
authors.affiliation: Respiratory Department, 208th Hospital of the Chinese PLA, Changchun 130062, People's Republic of China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Li
authors.affiliation: Shandong Provincial Key Laboratory of Biochemical Analysis, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, Shandong, People's Republic of China.
authors.forename: Wenyao
authors.initials: W
authors.lastname: Ding
authors.affiliation: Shandong Provincial Key Laboratory of Biochemical Analysis, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, Shandong, People's Republic of China. Electronic address: yangxiaoyan_zh@126.com.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Yang
mesh_terms: D001360:Avidin / Q000737:chemistry* / Q000378:metabolism; D001483:Base Sequence; D055162:Biocatalysis; D015374:Biosensing Techniques / Q000379:methods*; D001710:Biotin / Q000737:chemistry* / Q000378:metabolism; D004396:Coloring Agents / Q000737:chemistry; D021881:DNA, Catalytic / Q000235:genetics / Q000378:metabolism; D065546:Drug Liberation; D007854:Lead / Q000032:analysis* / Q000737:chemistry; D053758:Nanoparticles / Q000737:chemistry*; D016062:Porosity; D012822:Silicon Dioxide / Q000737:chemistry*; D014867:Water / Q000737:chemistry
chemical_list: D004396:Coloring Agents; D021881:DNA, Catalytic; D014867:Water; D001360:Avidin; D007854:Lead; D001710:Biotin; D012822:Silicon Dioxide
keywords: DNAzyme; Fluorescence; Lead ion; Mesoporous silica nanoparticles; Stimulus–release systems