title: Bioprocess development for the production of sonorensin by Bacillus sonorensis MT93 and its application as a food preservative.
abstract: Media composition and environmental conditions were optimized using statistical tools, Plackett Burman design and response surface methodology, to maximize the yield of a bacteriocin, named as sonorensin, from a new marine isolate Bacillus sonorensis MT93 showing broad spectrum of antimicrobial activity. Under optimized conditions, MT93 produced 15-fold higher yield of sonorensin compared to that under initial fermentation conditions. As oxygen supply is a critical parameter controlling growth and product formation in aerobic bioprocesses and used as a parameter for bioprocess scale up, the effects of oxygen transfer, in terms of volumetric oxygen transfer coefficient (kLa), on production of sonorensin was investigated using optimized medium composition in a bioreactor. Studies on effectiveness of sonorensin against Staphylococcus aureus and Listeria monocytogenes in fruit juice and as a preservative in pasteurized milk demonstrated its potential as a biopreservative in fruit products and shelf life extender of the pasteurized milk.
authors.affiliation: Biochemical Engineering Research and Process Development Centre, CSIR-Institute of Microbial Technology, Sector-39A, Chandigarh 160036, India.
authors.forename: Lipsy
authors.initials: L
authors.lastname: Chopra
authors.affiliation: Biochemical Engineering Research and Process Development Centre, CSIR-Institute of Microbial Technology, Sector-39A, Chandigarh 160036, India.
authors.forename: Gurdeep
authors.initials: G
authors.lastname: Singh
authors.affiliation: Biochemical Engineering Research and Process Development Centre, CSIR-Institute of Microbial Technology, Sector-39A, Chandigarh 160036, India.
authors.forename: Kautilya Kumar
authors.initials: KK
authors.lastname: Jena
authors.affiliation: Biochemical Engineering Research and Process Development Centre, CSIR-Institute of Microbial Technology, Sector-39A, Chandigarh 160036, India.
authors.forename: Himanshu
authors.initials: H
authors.lastname: Verma
authors.affiliation: Biochemical Engineering Research and Process Development Centre, CSIR-Institute of Microbial Technology, Sector-39A, Chandigarh 160036, India. Electronic address: debsahoo@imtech.res.in.
authors.forename: Debendra K
authors.initials: DK
authors.lastname: Sahoo
mesh_terms: D000332:Aerobiosis; D001407:Bacillus / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D001430:Bacteriocins / Q000096:biosynthesis*; D061249:Batch Cell Culture Techniques; D019149:Bioreactors / Q000382:microbiology; D001709:Biotechnology / Q000379:methods*; D005285:Fermentation; D005520:Food Preservatives; D006863:Hydrogen-Ion Concentration; D008962:Models, Theoretical; D009561:Nisin; D010100:Oxygen / Q000032:analysis; D015203:Reproducibility of Results; D013696:Temperature; D013997:Time Factors
chemical_list: D001430:Bacteriocins; D005520:Food Preservatives; C000610567:sonorensin protein, Bacillus sonorensis; D009561:Nisin; D010100:Oxygen
keywords: Bacillus sonorensis; Biopreservative; Optimization; Sonorensin; Volumetric oxygen transfer coefficient