title: Clustering of clinical and environmental Escherichia coli O104 isolates using the DiversiLab™ repetitive sequence-based PCR system.
abstract: The DiversiLab™ rep-PCR system was used to amplify DNA regions of 28 well-characterized Escherichia coli O104 strains to generate a digital DNA fingerprint profile for strain differentiation. E. coli O104 strains from human stools and other sources were examined. The results indicate that this system can cluster similar O104 strains rapidly.
authors.affiliation: Department of Biological Sciences, California State University, East Bay, 25800 Carlos Bee Blvd., Hayward, CA, 94542, USA.
authors.forename: N M
authors.initials: NM
authors.lastname: Herbold
authors.forename: L M
authors.initials: LM
authors.lastname: Clotilde
authors.forename: K M
authors.initials: KM
authors.lastname: Anderson
authors.forename: J
authors.initials: J
authors.lastname: Kase
authors.forename: G L
authors.initials: GL
authors.lastname: Hartman
authors.forename: S
authors.initials: S
authors.lastname: Himathongkham
authors.forename: A
authors.initials: A
authors.lastname: Lin
authors.forename: C R
authors.initials: CR
authors.lastname: Lauzon
mesh_terms: D016000:Cluster Analysis; D004269:DNA, Bacterial; D004783:Environmental Microbiology; D004927:Escherichia coli Infections / Q000382:microbiology; D006801:Humans; D016133:Polymerase Chain Reaction; D012091:Repetitive Sequences, Nucleic Acid; D054323:Shiga-Toxigenic Escherichia coli / Q000145:classification* / Q000235:genetics*
chemical_list: D004269:DNA, Bacterial