title: Cascading effects from survival to physiological activities, and gene expression of heat shock protein 90 on the abalone Haliotis discus hannai responding to continuous thermal stress.
abstract: Increasing temperatures can be a significant stressor for aquatic organisms. Abalones, a type of large marine gastropods, are the most commercially important species in aquaculture for Asia. To evaluate the potential ecological risk posed by temperature stress, we measured biological responses such as survival rate, adhesion ability (falling rate), and foot abnormalities in the abalone Haliotis discus hannai. Additionally, biochemical and molecular responses were evaluated in H. discus hannai exposed to various temperature gradients. The survival rate was reduced in abalones exposed to relative high temperatures (more than 26 °C). Increased temperature stress induced a higher falling rate and abnormal foot structure. Furthermore, increased antioxidant enzyme activities were observed in abalones exposed to relative high temperatures (26 and 28 °C). The activities of superoxide dismutase were induced in a time-dependent manner after high temperature stress. Generally, heat shock protein 90 also increased significantly in H. discus hannai exposed to temperature gradients (more than 24 °C) for 12 h. These results provide valuable information regarding stress responses to increased temperatures, in H. discus hannai: adverse biological and molecular outcomes could be utilized as risk assessments and stress monitoring of marine ecosystems under increased water temperatures.
authors.affiliation: Faculty of Marine Technology, Chonnam National University, Yeosu 550-749, Republic of Korea.
authors.forename: Kiyun
authors.initials: K
authors.lastname: Park
authors.affiliation: Department of Aqualife Medicine, Chonnam National University, Yeosu 550-749, Republic of Korea.
authors.forename: Jung Sick
authors.initials: JS
authors.lastname: Lee
authors.affiliation: Department of Aquatic Life Medicine, Pukyong National University, Busan 608-737, Republic of Korea.
authors.forename: Ju-Chan
authors.initials: JC
authors.lastname: Kang
authors.affiliation: Department of Marine Life Science and Aquaculture, Gangwon Provincial College, Gangneung 210-804, Republic of Korea.
authors.forename: Jae Won
authors.initials: JW
authors.lastname: Kim
authors.affiliation: Faculty of Marine Technology, Chonnam National University, Yeosu 550-749, Republic of Korea. Electronic address: iskwak@chonnam.ac.kr.
authors.forename: Ihn-Sil
authors.initials: IS
authors.lastname: Kwak
mesh_terms: D000818:Animals; D002374:Catalase / Q000235:genetics / Q000378:metabolism; D049851:Gastropoda / Q000201:enzymology / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D005786:Gene Expression Regulation; D018841:HSP90 Heat-Shock Proteins / Q000235:genetics* / Q000378:metabolism; D006358:Hot Temperature / Q000009:adverse effects*; D008136:Longevity; D013312:Stress, Physiological / Q000235:genetics / Q000502:physiology*; D013482:Superoxide Dismutase / Q000235:genetics / Q000378:metabolism
chemical_list: D018841:HSP90 Heat-Shock Proteins; D002374:Catalase; D013482:Superoxide Dismutase
keywords: Abnormal foot structure; Haliotis discus hannai; Heat shock protein 90; Superoxide dismutase; Water temperature