title: Freezing/thawing pretreatment coupled with biological process of thermophilic Geobacillus sp. G1: Acceleration on waste activated sludge hydrolysis and acidification.
abstract: A novel pretreatment method combining freezing/thawing with Geobacillus sp. G1 was employed to pretreat waste activated sludge (WAS) for enhancing the WAS hydrolysis and subsequent short-chain fatty acids (SCFAs) production. Results showed that freezing/thawing combined with Geobacillus sp. G1 pretreatment achieved the maximal concentrations of soluble protein from 40±6mg COD/L (non-pretreated) to 1226±24mg COD/L (pretreated), and accumulated SCFAs concentration increased from 248±81mg COD/L to 3032±53mg COD/L. Excitation emission matrix (EEM) fluorescence spectroscopy revealed the highest fluorescence intensity (FI) of protein-like substances, which was the dominant fluorescent organic matters, indicating the synergistic effect of freezing/thawing and Geobacillus sp. G1 pretreatment on organics hydrolysis. High-throughput pyrosequencing analysis investigated that the abundance of bacteria responsible for WAS hydrolysis (such as Clostridium and Caloramator) and SCFAs production (such as Parabacteroides and Bacterodies) was greatly enhanced due to the novel pretreatment method used.
authors.affiliation: State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology (SKLUWRE, HIT), Harbin, China.
authors.forename: Chunxue
authors.initials: C
authors.lastname: Yang
authors.affiliation: Key Laboratory of Environmental Biotechnology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, China.
authors.forename: Wenzong
authors.initials: W
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology (SKLUWRE, HIT), Harbin, China.
authors.forename: Zhangwei
authors.initials: Z
authors.lastname: He
authors.affiliation: State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology (SKLUWRE, HIT), Harbin, China.
authors.forename: Sangeetha
authors.initials: S
authors.lastname: Thangavel
authors.affiliation: State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology (SKLUWRE, HIT), Harbin, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology (SKLUWRE, HIT), Harbin, China.
authors.forename: Aijuan
authors.initials: A
authors.lastname: Zhou
authors.affiliation: State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology (SKLUWRE, HIT), Harbin, China; Key Laboratory of Environmental Biotechnology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, China. Electronic address: waj0578@hit.edu.cn.
authors.forename: Aijie
authors.initials: A
authors.lastname: Wang
mesh_terms: D000143:Acids / Q000378:metabolism*; D001686:Biological Phenomena; D052918:Environmental Restoration and Remediation / Q000379:methods; D005232:Fatty Acids, Volatile / Q000378:metabolism; D005615:Freezing; D056506:Geobacillus / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D012722:Sewage / Q000737:chemistry / Q000382:microbiology*; D013050:Spectrometry, Fluorescence; D018505:Waste Management / Q000379:methods*
chemical_list: D000143:Acids; D005232:Fatty Acids, Volatile; D012722:Sewage
keywords: Excitation emission matrix (EEM); Freezing/thawing pretreatment; High-throughput pyrosequencing analysis; Thermophilic Geobacillus sp. G1; Waste activated sludge (WAS)