title: Intranasal immunization of recombinant Lactococcus lactis induces protection against H5N1 virus in ferrets.
abstract: The increasing outbreaks of highly pathogenic avian influenza A (HPAI) H5N1 viruses in birds and human bring out an urgent need to develop a safe and effective vaccine to control and prevent H5N1 infection. Lactococcus lactis (L. lactis) based vaccine platform is a promising approach for mucosal H5N1 vaccine development. Intranasal immunization is the potential to induce mucosal immune response which is associated with protective immunity. To develop a safe and effective mucosal vaccine against HAPI H5N1, we extended our previous study by evaluating the immunogenicity of L. lactis-psA-HA1 in the absence of adjuvant via intranasal route in the ferret model. Ferrets administered intranasally with L. lactis-pgsA-HA1 could elicit robust humoral and mucosal immune responses, as well as significant HI titers. Importantly, ferrets were completely protected from H5N1 virus challenge. These findings suggest that L. lactis-pgsA-HA1 can be considered an alternative mucosal vaccine during A/H5N1 pandemic.
authors.affiliation: Department of Biotechnology, College of Life Science and Food Engineering, Nanchang University, Jiangxi 330031, China; Department of Biomedical Engineering, State University of New York, Binghamton, NY 13902, USA. Electronic address: hlei@binghamton.edu.
authors.forename: Han
authors.initials: H
authors.lastname: Lei
authors.affiliation: Department of Biotechnology, College of Life Science and Food Engineering, Nanchang University, Jiangxi 330031, China.
authors.forename: Xiaojue
authors.initials: X
authors.lastname: Peng
authors.affiliation: Department of Biotechnology, College of Life Science and Food Engineering, Nanchang University, Jiangxi 330031, China.
authors.forename: Jiexiu
authors.initials: J
authors.lastname: Ouyang
authors.affiliation: Department of Biotechnology, College of Life Science and Food Engineering, Nanchang University, Jiangxi 330031, China.
authors.forename: Daxian
authors.initials: D
authors.lastname: Zhao
authors.affiliation: Department of Biotechnology, College of Life Science and Food Engineering, Nanchang University, Jiangxi 330031, China.
authors.forename: Huifeng
authors.initials: H
authors.lastname: Jiao
authors.affiliation: Department of Biotechnology, College of Life Science and Food Engineering, Nanchang University, Jiangxi 330031, China.
authors.forename: Handing
authors.initials: H
authors.lastname: Shu
authors.affiliation: Department of Biotechnology, College of Life Science and Food Engineering, Nanchang University, Jiangxi 330031, China.
authors.forename: Xinqi
authors.initials: X
authors.lastname: Ge
mesh_terms: D000281:Administration, Intranasal; D000818:Animals; D057134:Antibodies, Neutralizing / Q000097:blood / Q000276:immunology; D000914:Antibodies, Viral / Q000097:blood / Q000276:immunology; D005260:Female; D005289:Ferrets; D006385:Hemagglutination Inhibition Tests; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000235:genetics / Q000276:immunology; D007114:Immunization; D007070:Immunoglobulin A / Q000097:blood / Q000276:immunology; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology; D053124:Influenza A Virus, H5N1 Subtype / Q000235:genetics* / Q000276:immunology*; D007252:Influenza Vaccines / Q000008:administration & dosage; D013294:Lactococcus lactis / Q000235:genetics* / Q000276:immunology*; D009976:Orthomyxoviridae Infections / Q000401:mortality / Q000517:prevention & control*
chemical_list: D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D019267:Hemagglutinin Glycoproteins, Influenza Virus; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007252:Influenza Vaccines
keywords: Ferrets; H5N1 virus; Intranasal immunization; L. lactis-pgsA-HA1