title: Genomic and transcriptomic characterization of the transcription factor family R2R3-MYB in soybean and its involvement in the resistance responses to Phakopsora pachyrhizi.
abstract: Myb genes constitute one of the largest transcription factor families in the plant kingdom. Soybean MYB transcription factors have been related to the plant response to biotic stresses. Their involvement in response to Phakopsora pachyrhizi infection has been reported by several transcriptional studies. Due to their apparently highly diverse functions, these genes are promising targets for developing crop varieties resistant to diseases. In the present study, the identification and phylogenetic analysis of the soybean R2R3-MYB (GmMYB) transcription factor family was performed and the expression profiles of these genes under biotic stress were determined. GmMYBs were identified from the soybean genome using bioinformatic tools, and their putative functions were determined based on the phylogenetic tree and classified into subfamilies using guides AtMYBs describing known functions. The transcriptional profiles of GmMYBs upon infection with different pathogen were revealed by in vivo and in silico analyses. Selected target genes potentially involved in disease responses were assessed by RT-qPCR after different times of inoculation with P. pachyrhizi using different genetic backgrounds related to resistance genes (Rpp2 and Rpp5). R2R3-MYB transcription factors related to lignin synthesis and genes responsive to chitin were significantly induced in the resistant genotypes.
authors.affiliation: Department of Biochemistry and Biotechnology, Universidade Estadual de Londrina, Celso Garcia Cid - Pr 445 Highway, Km 380, 86.057-970, Londrina, Paraná, Brazil; Department of Biological Sciences, Universidade Estadual de Maringá, Av. Colombo Avenue, Number 5.790, Jd. Universitário, 87.020-900, Maringa, Paraná, Brazil. Electronic address: luciano@cnpso.embrapa.br.
authors.forename: Luciano N
authors.initials: LN
authors.lastname: Aoyagi
authors.affiliation: Departament of Computer Science, Universidade Tecnológica Federal do Paraná, Alberto Carazzai Avenue, Number 1640, 86.300-000, Cornélio Procópio, Parana, Brazil; Department of General Biology, Universidade Estadual de Londrina, Celso Garcia Cid Road, PR 445, Km 380, P.O. Box 6001, 86051-990, Brazil; Brazilian Agricultural Research Corporation - EMBRAPA, P.O. Box 231, Carlos João Strass Highway - Distrito de Warta, 86.001-970, Londrina, Paraná, Brazil. Electronic address: valopes@cnpso.embrapa.br.
authors.forename: Valéria S
authors.initials: VS
authors.lastname: Lopes-Caitar
authors.affiliation: Department of Biological Sciences, Universidade Estadual do Norte do Paraná, Bandeirantes-Brazil, BR-369 highway, Km 54, Vila Maria, 86.360-000, Bandeirantes, Paraná, Brazil. Electronic address: mayra@uenp.edu.br.
authors.forename: Mayra C C G
authors.initials: MCCG
authors.lastname: de Carvalho
authors.affiliation: Department of Biological Sciences, Universidade Estadual de Maringá, Av. Colombo Avenue, Number 5.790, Jd. Universitário, 87.020-900, Maringa, Paraná, Brazil; Brazilian Agricultural Research Corporation - EMBRAPA, P.O. Box 231, Carlos João Strass Highway - Distrito de Warta, 86.001-970, Londrina, Paraná, Brazil. Electronic address: luana.darben@cnpso.embrapa.br.
authors.forename: Luana M
authors.initials: LM
authors.lastname: Darben
authors.affiliation: Brazilian Agricultural Research Corporation - EMBRAPA, P.O. Box 231, Carlos João Strass Highway - Distrito de Warta, 86.001-970, Londrina, Paraná, Brazil. Electronic address: polizel@cnpso.embrapa.br.
authors.forename: Adriana
authors.initials: A
authors.lastname: Polizel-Podanosqui
authors.affiliation: Brazilian Agricultural Research Corporation - EMBRAPA, P.O. Box 231, Carlos João Strass Highway - Distrito de Warta, 86.001-970, Londrina, Paraná, Brazil. Electronic address: marcia.kuwahara@embrapa.br.
authors.forename: Marcia K
authors.initials: MK
authors.lastname: Kuwahara
authors.affiliation: Brazilian Agricultural Research Corporation - EMBRAPA, P.O. Box 231, Carlos João Strass Highway - Distrito de Warta, 86.001-970, Londrina, Paraná, Brazil. Electronic address: alexandre.nepomuceno@embrapa.br.
authors.forename: Alexandre L
authors.initials: AL
authors.lastname: Nepomuceno
authors.affiliation: Brazilian Agricultural Research Corporation - EMBRAPA, P.O. Box 231, Carlos João Strass Highway - Distrito de Warta, 86.001-970, Londrina, Paraná, Brazil. Electronic address: ricardo.abdelnoor@embrapa.br.
authors.forename: Ricardo V
authors.initials: RV
authors.lastname: Abdelnoor
authors.affiliation: Brazilian Agricultural Research Corporation - EMBRAPA, P.O. Box 231, Carlos João Strass Highway - Distrito de Warta, 86.001-970, Londrina, Paraná, Brazil. Electronic address: francismar.marcelino@embrapa.br.
authors.forename: Francismar C
authors.initials: FC
authors.lastname: Marcelino-Guimarães
mesh_terms: D001487:Basidiomycota / Q000502:physiology*; D003198:Computer Simulation; D060467:Disease Resistance / Q000235:genetics*; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D018745:Genome, Plant; D005838:Genotype; D005810:Multigene Family; D010802:Phylogeny; D010935:Plant Diseases / Q000235:genetics / Q000276:immunology / Q000382:microbiology; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D015203:Reproducibility of Results; D013025:Glycine max / Q000235:genetics* / Q000276:immunology / Q000382:microbiology*; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism; D014158:Transcription, Genetic; D059467:Transcriptome / Q000235:genetics*
chemical_list: D010940:Plant Proteins; D014157:Transcription Factors
keywords: Biotic stresses; GmMYB; Phakopsora pachyrhizi; Phylogeny; RT-qPCR; Resistance