title: An innovative membrane bioreactor for methane biohydroxylation.
abstract: In this study, a membrane bioreactor (MBR) was developed for efficient, safe microbial methane hydroxylation with Methylosinus trichosporium OB3b. This innovative MBR, which couples a bioreactor with two gas/liquid macroporous membrane contactors supplying the two gaseous substrates (methane and oxygen) was operated in fed-batch mode. The feasibility and the reproducibility of this new biohydroxylation process were first demonstrated. The mass transfer within this MBR was twice that observed in a batch reactor in similar conditions. The productivity reached with this MBR was 75±25mgmethanol(gdrycell)(-1)h(-1). Compared to the literature, this value is 35times higher than that obtained with the only other fed-batch membrane bioreactor reported, which was run with dense membranes, and is comparable to those obtained with bioreactors fed by bubble-spargers. However, in the latter case, an explosive gas mixture can be formed, a problem that is avoided with the MBR.
authors.affiliation: IEM (Institut Europeen des Membranes), UMR 5635 (CNRS-ENSCM-UM2), Universite Montpellier II, Place E. Bataillon, F-34095 Montpellier, France.
authors.forename: N
authors.initials: N
authors.lastname: Pen
authors.affiliation: IEM (Institut Europeen des Membranes), UMR 5635 (CNRS-ENSCM-UM2), Universite Montpellier II, Place E. Bataillon, F-34095 Montpellier, France. Electronic address: Laurence.Soussan@univ-montp2.fr.
authors.forename: L
authors.initials: L
authors.lastname: Soussan
authors.affiliation: IEM (Institut Europeen des Membranes), UMR 5635 (CNRS-ENSCM-UM2), Universite Montpellier II, Place E. Bataillon, F-34095 Montpellier, France.
authors.forename: M-P
authors.initials: MP
authors.lastname: Belleville
authors.affiliation: IEM (Institut Europeen des Membranes), UMR 5635 (CNRS-ENSCM-UM2), Universite Montpellier II, Place E. Bataillon, F-34095 Montpellier, France.
authors.forename: J
authors.initials: J
authors.lastname: Sanchez
authors.affiliation: IEM (Institut Europeen des Membranes), UMR 5635 (CNRS-ENSCM-UM2), Universite Montpellier II, Place E. Bataillon, F-34095 Montpellier, France.
authors.forename: C
authors.initials: C
authors.lastname: Charmette
authors.affiliation: IEM (Institut Europeen des Membranes), UMR 5635 (CNRS-ENSCM-UM2), Universite Montpellier II, Place E. Bataillon, F-34095 Montpellier, France.
authors.forename: D
authors.initials: D
authors.lastname: Paolucci-Jeanjean
mesh_terms: D061249:Batch Cell Culture Techniques; D055162:Biocatalysis / Q000187:drug effects; D019149:Bioreactors; D003470:Culture Media; D005240:Feasibility Studies; D006900:Hydroxylation; D007700:Kinetics; D008567:Membranes, Artificial; D053858:Metabolic Networks and Pathways; D008697:Methane / Q000378:metabolism*; D020602:Methylosinus trichosporium / Q000378:metabolism*; D010084:Oxidation-Reduction; D015203:Reproducibility of Results
chemical_list: D003470:Culture Media; D008567:Membranes, Artificial; D008697:Methane
keywords: Membrane bioreactor; Methane; Methanol; Methylosinus trichosporium OB3b; Microbial hydroxylation