title: MiR-92a inhibits porcine ovarian granulosa cell apoptosis by targeting Smad7 gene.
abstract: Smad7 has a key role in apoptosis of mammalian ovarian granulosa cells (GCs), as it antagonizes and fine-tunes transforming growth factor β (TGFβ) signaling. This study demonstrates that miR-92a regulates GC apoptosis in pig ovaries by targeting Smad7 directly. The expression level of miR-92a was down-regulated in atretic porcine follicles, whereas miR-92a expression led to inhibition of GC apoptosis. The Smad7 gene was identified as a direct target of miR-92a using a dual-luciferase reporter assay. Transfection of GCs with miR-92a mimics decreased Smad7 mRNA and protein levels, whereas expression of an miR-92a inhibitor in GCs had the opposite effect. In addition, knockdown of Smad7 prevented GC apoptosis in cells that expressed the miR-92a inhibitor.
authors.affiliation: College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, People’s Republic of China.
authors.forename: Jiying
authors.initials: J
authors.lastname: Liu
authors.forename: Wang
authors.initials: W
authors.lastname: Yao
authors.forename: Yong
authors.initials: Y
authors.lastname: Yao
authors.forename: Xing
authors.initials: X
authors.lastname: Du
authors.forename: Jilong
authors.initials: J
authors.lastname: Zhou
authors.forename: Baiquan
authors.initials: B
authors.lastname: Ma
authors.forename: Honglin
authors.initials: H
authors.lastname: Liu
authors.forename: Qifa
authors.initials: Q
authors.lastname: Li
authors.forename: Zengxiang
authors.initials: Z
authors.lastname: Pan
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000235:genetics*; D005260:Female; D006107:Granulosa Cells / Q000166:cytology*; D035683:MicroRNAs / Q000235:genetics*; D010053:Ovary / Q000166:cytology*; D051906:Smad7 Protein / Q000235:genetics*; D013552:Swine
chemical_list: D035683:MicroRNAs; D051906:Smad7 Protein