title: Preparative purification of polyphenols from sweet potato (Ipomoea batatas L.) leaves by AB-8 macroporous resins.
abstract: In this study, the adsorption properties of AB-8 macroporous resin for sweet potato leaf polyphenols was investigated. The adsorption mechanism was elaborated by the Langmuir and Freundlich equations, and the purification parameters were optimised by adsorption and desorption tests. The constituents and their contents of the purified products were analysed, and the antioxidant activities were determined. The results showed that the optimal processing parameters were as follows: an initial polyphenol concentration of 2.0mg chlorogenic acid equivalent (CAE)/ml, pH 3.0, an ethanol desorption solution concentration of 70% (v/v) and a flow rate for feeding and elution of 1BV/h. The purified products mainly contained eight phenolic constituents and the contents of three di-caffeoylquinic acids were relatively higher than the other constituents. The purified products possessed strong antioxidant activities. In conclusion, purification by AB-8 macroporous resin was highly efficient, economic and environmentally friendly and has a great industrial production potential.
authors.affiliation: Laboratory of Food Chemistry and Nutrition Science, Institute of Agro-Products Processing Science and Technology, Chinese Academy of Agricultural Sciences, Key Laboratory of Agro-Products Processing, Ministry of Agriculture, No. 2 Yuan Ming Yuan West Road, Haidian District, Beijing 100193, PR China.
authors.forename: Lisha
authors.initials: L
authors.lastname: Xi
authors.affiliation: Laboratory of Food Chemistry and Nutrition Science, Institute of Agro-Products Processing Science and Technology, Chinese Academy of Agricultural Sciences, Key Laboratory of Agro-Products Processing, Ministry of Agriculture, No. 2 Yuan Ming Yuan West Road, Haidian District, Beijing 100193, PR China. Electronic address: mutaihua@126.com.
authors.forename: Taihua
authors.initials: T
authors.lastname: Mu
authors.affiliation: Laboratory of Food Chemistry and Nutrition Science, Institute of Agro-Products Processing Science and Technology, Chinese Academy of Agricultural Sciences, Key Laboratory of Agro-Products Processing, Ministry of Agriculture, No. 2 Yuan Ming Yuan West Road, Haidian District, Beijing 100193, PR China.
authors.forename: Hongnan
authors.initials: H
authors.lastname: Sun
mesh_terms: D000327:Adsorption; D002851:Chromatography, High Pressure Liquid; D027723:Ipomoea batatas / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry; D059808:Polyphenols / Q000302:isolation & purification*; D016062:Porosity; D012116:Resins, Plant / Q000737:chemistry*
chemical_list: D059808:Polyphenols; D012116:Resins, Plant
keywords: Antioxidant activity; Macroporous resins; Polyphenols; Purification; Sweet potato leaves