title: Cellular targets for improved manufacturing of virus-based biopharmaceuticals in animal cells.
abstract: The past decade witnessed the entry into the market of new virus-based biopharmaceuticals produced in animal cells such as oncolytic vectors, virus-like particle vaccines, and gene transfer vectors. Therefore, increased attention and investment to optimize cell culture processes towards enhanced manufacturing of these bioproducts is anticipated. Herein, we review key findings on virus-host interactions that have been explored in cell culture optimization. Approaches supporting improved productivity or quality of vector preparations are discussed, mainly focusing on medium design and genetic manipulation. This review provides an integrated outline for current and future efforts in exploring cellular targets for the optimization of cell culture manufacturing of virus-based biopharmaceuticals.
authors.affiliation: iBET - Instituto de Biologia Experimental e Tecnológica, Apartado 12, 2781-901 Oeiras, Portugal; Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, Av. da República, 2780-157 Oeiras, Portugal.
authors.forename: Ana F
authors.initials: AF
authors.lastname: Rodrigues
authors.affiliation: iBET - Instituto de Biologia Experimental e Tecnológica, Apartado 12, 2781-901 Oeiras, Portugal; FCT-UNL, P-2829-516 Caparica, Portugal.
authors.forename: Manuel J T
authors.initials: MJ
authors.lastname: Carrondo
authors.affiliation: iBET - Instituto de Biologia Experimental e Tecnológica, Apartado 12, 2781-901 Oeiras, Portugal; Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, Av. da República, 2780-157 Oeiras, Portugal.
authors.forename: Paula M
authors.initials: PM
authors.lastname: Alves
authors.affiliation: iBET - Instituto de Biologia Experimental e Tecnológica, Apartado 12, 2781-901 Oeiras, Portugal; Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, Av. da República, 2780-157 Oeiras, Portugal. Electronic address: avalente@itqb.unl.pt.
authors.forename: Ana S
authors.initials: AS
authors.lastname: Coroadinha
mesh_terms: D000818:Animals; D001688:Biological Products / Q000302:isolation & purification / Q000378:metabolism*; D001709:Biotechnology / Q000379:methods / Q000639:trends; D018929:Cell Culture Techniques / Q000379:methods; D002478:Cells, Cultured; D003470:Culture Media / Q000737:chemistry; D005818:Genetic Engineering / Q000379:methods; D006790:Host-Parasite Interactions; D013678:Technology, Pharmaceutical / Q000379:methods* / Q000639:trends; D014780:Viruses / Q000235:genetics* / Q000254:growth & development* / Q000302:isolation & purification
chemical_list: D001688:Biological Products; D003470:Culture Media
keywords: biochemical pathways; genetic manipulation; medium design; vaccines; viral vectors; virus–host interactions