title: Andrographolide interferes quorum sensing to reduce cell damage caused by avian pathogenic Escherichia coli.
abstract: Avian pathogenic Escherichia coli (APEC) induce septicemia in chickens by invading type II pneumocytes to breach the blood-air barrier. The virulence of APEC can be regulated by quorum sensing (QS). Andrographolide is a QS inhibitor of Pseudomonas aeruginosa (P. aeruginosa). Therefore, we investigate whether andrographolide inhibits the injury of chicken type II pneumocytes by avian pathogenic E. coli O78 (APEC-O78) by disrupting the bacterial QS system. The results showed that sub-MIC of andrographolide significantly reduced the release of lactate dehydrogenase (LDH), F-actin cytoskeleton polymerization, and the degree of the adherence to chicken type II pneumocytes induced by APEC-O78. Further, we found that andrographolide significantly decreased the autoinducer-2 (AI-2) activity and the expression of virulence factors of APEC-O78. These results suggest that andrographolide reduce the pathogenicity of APEC-O78 in chicken type II pneumocytes by interfering QS and decreasing virulence. These results provide new evidence for colibacillosis prevention methods in chickens.
authors.affiliation: Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Jilin University, No. 5333, Xi'an Road, Changchun, Jilin 130062, China.
authors.forename: Xun
authors.initials: X
authors.lastname: Guo
authors.affiliation: Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Jilin University, No. 5333, Xi'an Road, Changchun, Jilin 130062, China; Beijing Keepyoung Technology Co. Ltd., Shangdi East Road, Haidian District, Beijing 100085, China.
authors.forename: Li-Yan
authors.initials: LY
authors.lastname: Zhang
authors.affiliation: Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Jilin University, No. 5333, Xi'an Road, Changchun, Jilin 130062, China.
authors.forename: Shuai-Cheng
authors.initials: SC
authors.lastname: Wu
authors.affiliation: Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Jilin University, No. 5333, Xi'an Road, Changchun, Jilin 130062, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Xia
authors.affiliation: Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Jilin University, No. 5333, Xi'an Road, Changchun, Jilin 130062, China.
authors.forename: Yun-Xing
authors.initials: YX
authors.lastname: Fu
authors.affiliation: Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Jilin University, No. 5333, Xi'an Road, Changchun, Jilin 130062, China.
authors.forename: Yong-Li
authors.initials: YL
authors.lastname: Wu
authors.affiliation: Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Jilin University, No. 5333, Xi'an Road, Changchun, Jilin 130062, China.
authors.forename: Chun-Qing
authors.initials: CQ
authors.lastname: Leng
authors.affiliation: Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Jilin University, No. 5333, Xi'an Road, Changchun, Jilin 130062, China.
authors.forename: Peng-Fei
authors.initials: PF
authors.lastname: Yi
authors.affiliation: Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Jilin University, No. 5333, Xi'an Road, Changchun, Jilin 130062, China.
authors.forename: Hai-Qing
authors.initials: HQ
authors.lastname: Shen
authors.affiliation: Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Jilin University, No. 5333, Xi'an Road, Changchun, Jilin 130062, China.
authors.forename: Xu-Bin
authors.initials: XB
authors.lastname: Wei
authors.affiliation: Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Jilin University, No. 5333, Xi'an Road, Changchun, Jilin 130062, China. Electronic address: fubendong96@163.com.
authors.forename: Ben-Dong
authors.initials: BD
authors.lastname: Fu
mesh_terms: D000818:Animals; D002645:Chickens / Q000382:microbiology*; D004224:Diterpenes / Q000494:pharmacology*; D004926:Escherichia coli / Q000472:pathogenicity*; D004927:Escherichia coli Infections / Q000188:drug therapy / Q000382:microbiology / Q000662:veterinary*; D011201:Poultry Diseases / Q000188:drug therapy* / Q000382:microbiology; D053038:Quorum Sensing / Q000187:drug effects*; D014774:Virulence / Q000187:drug effects; D037521:Virulence Factors / Q000378:metabolism
chemical_list: D004224:Diterpenes; D037521:Virulence Factors; C030419:andrographolide
keywords: Andrographolide; Avian pathogenic Escherichia coli; Chicken type II pneumocyte; Quorum sensing