title: N-hexanoyl-L-homoserine lactone-degrading Pseudomonas aeruginosa PsDAHP1 protects zebrafish against Vibrio parahaemolyticus infection.
abstract: Four strains of N-hexanoyl-L-homoserine lactone (AHL)-degrading Pseudomonas spp., named PsDAHP1, PsDAHP2, PsDAHP3, and PsDAHP4 were isolated and identified from the intestine of Fenneropenaeus indicus. PsDAHP1 showed the highest AHL-degrading activity among the four isolates. PsDAHP1 inhibited biofilm-forming exopolysaccharide and altered cell surface hydrophobicity of virulent green fluorescent protein (GFP)-tagged Vibrio parahaemolyticus DAHV2 (GFP-VpDAHV2). Oral administration of PsDAHP1 significantly reduced zebrafish mortality caused by GFP-VpDAHV2 challenge, and inhibited colonisation of GFP-VpDAHV2 in the gills and intestine of zebrafish as evidence by confocal laser scanning microscope and selective plating. Furthermore, zebrafish receiving PsDAHP1-containing feed had increased phagocytic cells of its leucocytes, increased serum activities of superoxide dismutase and lysozyme. The results suggest that Pseudomonas aeruginosa PsDAHP1 could protect zebrafish from V. parahaemolyticus infection by inhibiting biofilm formation and enhancing defence mechanisms of the fish.
authors.affiliation: Crustacean Molecular Biology and Genomics Lab, Department of Animal Health and Management, Alagappa University, Karaikudi 630 004, Tamil Nadu, India.
authors.forename: Gopalakrishnan
authors.initials: G
authors.lastname: Vinoj
authors.affiliation: Central Marine Fisheries Research Institute (CMFRI), Marine Fisheries Post, Mandapam Camp, 623520 Tamil Nadu, India.
authors.forename: Rengarajan
authors.initials: R
authors.lastname: Jayakumar
authors.affiliation: Department of Aquaculture, College of Life Sciences, National Taiwan Ocean University, 202, Keelung, Taiwan, ROC.
authors.forename: Jiann-Chu
authors.initials: JC
authors.lastname: Chen
authors.affiliation: Center of Excellence for Shrimp Molecular Biology and Biotechnology (Centex Shrimp), Mahidol University, Bangkok 10400, Thailand; Aquatic Animal Biotechnology Research Center, Faculty of Science and Industrial Technology, Prince of Songkla University, Surat Thani Campus, Surat Thani 84100, Thailand.
authors.forename: Boonsirm
authors.initials: B
authors.lastname: Withyachumnarnkul
authors.affiliation: Crustacean Molecular Biology and Genomics Lab, Department of Animal Health and Management, Alagappa University, Karaikudi 630 004, Tamil Nadu, India.
authors.forename: Sathappan
authors.initials: S
authors.lastname: Shanthi
authors.affiliation: Crustacean Molecular Biology and Genomics Lab, Department of Animal Health and Management, Alagappa University, Karaikudi 630 004, Tamil Nadu, India. Electronic address: vaseeharanb@gmail.com.
authors.forename: Baskaralingam
authors.initials: B
authors.lastname: Vaseeharan
mesh_terms: D015107:4-Butyrolactone / Q000031:analogs & derivatives / Q000378:metabolism; D000704:Analysis of Variance; D000818:Animals; D018441:Biofilms / Q000187:drug effects; D005393:Fish Diseases / Q000382:microbiology* / Q000517:prevention & control*; D049452:Green Fluorescent Proteins; D057927:Hydrophobic and Hydrophilic Interactions / Q000187:drug effects; D018613:Microscopy, Confocal / Q000662:veterinary; D033561:Penaeidae / Q000382:microbiology*; D011550:Pseudomonas aeruginosa / Q000235:genetics / Q000378:metabolism*; D013045:Species Specificity; D014735:Vibrio Infections / Q000517:prevention & control / Q000662:veterinary*; D014736:Vibrio parahaemolyticus; D015027:Zebrafish
chemical_list: C104908:N-hexanoyl-L-homoserine lactone; D049452:Green Fluorescent Proteins; D015107:4-Butyrolactone
keywords: AHL; Biofilm; Green fluorescent protein; Pseudomonas; zebrafish