title: Fourier-ring descriptor to characterize rare circulating cells from images generated using immunofluorescence microscopy.
abstract: We address the problem of subclassification of rare circulating cells using data driven feature selection from images of candidate circulating tumor cells from patients diagnosed with breast, prostate, or lung cancer. We determine a set of low level features which can differentiate among candidate cell types. We have implemented an image representation based on concentric Fourier rings (FRDs) which allow us to exploit size variations and morphological differences among cells while being rotationally invariant. We discuss potential clinical use in the context of treatment monitoring for cancer patients with metastatic disease.
authors.affiliation: Department of Mathematics, Colorado State University, 841 Oval Drive, Fort Collins, CO 80523, United States. Electronic address: emerson@math.colostate.edu.
authors.forename: Tegan
authors.initials: T
authors.lastname: Emerson
authors.affiliation: Department of Mathematics, Colorado State University, 841 Oval Drive, Fort Collins, CO 80523, United States. Electronic address: kirby@math.colostate.edu.
authors.forename: Michael
authors.initials: M
authors.lastname: Kirby
authors.affiliation: The Scripps Clinic, Department of Pathology, 10666 N Torrey Pines Road, La Jolla, CA 92037, United States. Electronic address: Bethel.Kelly@scrippshealth.org.
authors.forename: Kelly
authors.initials: K
authors.lastname: Bethel
authors.affiliation: The Scripps Research Institute, The Kuhn Lab, 10550 N Torrey Pines Road, La Jolla, CA 92037, United States. Electronic address: anandk@scripps.edu.
authors.forename: Anand
authors.initials: A
authors.lastname: Kolatkar
authors.affiliation: The Scripps Research Institute, The Kuhn Lab, 10550 N Torrey Pines Road, La Jolla, CA 92037, United States. Electronic address: mluttgen@scripps.edu.
authors.forename: Madelyn
authors.initials: M
authors.lastname: Luttgen
authors.affiliation: DigitalGlobe, Image Mining Group, 1601 Dry Creek Drive, Longmont, CO 80503, United States. Electronic address: svohara@ieee.org.
authors.forename: Stephen
authors.initials: S
authors.lastname: O'Hara
authors.affiliation: Department of Aerospace and Mechanical Engineering, University of Southern California Viterbi School of Engineering, Los Angeles, CA 90089, United States. Electronic address: newton@usc.edu.
authors.forename: Paul
authors.initials: P
authors.lastname: Newton
authors.affiliation: The Kuhn Lab, Department of Biological Sciences, University of Southern California, Los Angeles, CA 90089, United States. Electronic address: peter.kuhn@usc.edu.
authors.forename: Peter
authors.initials: P
authors.lastname: Kuhn
mesh_terms: D000465:Algorithms; D001185:Artificial Intelligence; D058948:Cell Tracking / Q000379:methods*; D005583:Fourier Analysis; D006801:Humans; D007089:Image Enhancement / Q000379:methods; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D008856:Microscopy, Fluorescence / Q000379:methods*; D009360:Neoplastic Cells, Circulating / Q000473:pathology*; D010363:Pattern Recognition, Automated / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013382:Subtraction Technique