title: H/D exchange of a15N labelled Tau fragment as measured by a simple Relax-EXSY experiment.
abstract: We present an equilibrium H/D exchange experiment to measure the exchange rates of labile amide protons in intrinsically unfolded proteins. By measuring the contribution of the H/D exchange to the apparent T1relaxation rates in solvents of different D2O content, we can easily derive the rates of exchange for rapidly exchanging amide protons. The method does not require double isotope labelling, is sensitive, and requires limited fitting of the data. We demonstrate it on a functional fragment of Tau, and provide evidence for the hydrogen bond formation of the phosphate moiety of Ser214 with its own amide proton in the same fragment phosphorylated by the PKA kinase.
authors.affiliation: CNRS UMR 8576, Unité de Glycobiologie Structurale et Fonctionnelle, Université des Sciences et Technologies de Lille 1, 59655 Villeneuve d'Ascq Cedex, France.
authors.forename: Juan
authors.initials: J
authors.lastname: Lopez
authors.affiliation: CNRS UMR 8576, Unité de Glycobiologie Structurale et Fonctionnelle, Université des Sciences et Technologies de Lille 1, 59655 Villeneuve d'Ascq Cedex, France.
authors.forename: Puneet
authors.initials: P
authors.lastname: Ahuja
authors.affiliation: CNRS UMR 8576, Unité de Glycobiologie Structurale et Fonctionnelle, Université des Sciences et Technologies de Lille 1, 59655 Villeneuve d'Ascq Cedex, France; CNRS USR 3078, Institut de Recherche Interdisciplinaire, Centre National de la Recherche Scientifique, 59655 Villeneuve d'Ascq, France.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Landrieu
authors.affiliation: CNRS UMR 8576, Unité de Glycobiologie Structurale et Fonctionnelle, Université des Sciences et Technologies de Lille 1, 59655 Villeneuve d'Ascq Cedex, France.
authors.forename: François-Xavier
authors.initials: FX
authors.lastname: Cantrelle
authors.affiliation: CNRS UMR 8576, Unité de Glycobiologie Structurale et Fonctionnelle, Université des Sciences et Technologies de Lille 1, 59655 Villeneuve d'Ascq Cedex, France.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Huvent
authors.affiliation: CNRS UMR 8576, Unité de Glycobiologie Structurale et Fonctionnelle, Université des Sciences et Technologies de Lille 1, 59655 Villeneuve d'Ascq Cedex, France. Electronic address: Guy.Lippens@univ-lille1.fr.
authors.forename: Guy
authors.initials: G
authors.lastname: Lippens
keywords: Amide protons; H/D exchange; Intrinsically unfolded protein; Phosphorylation