title: TALEN-mediated mutagenesis in zebrafish reveals a role for r-spondin 2 in fin ray and vertebral development.
abstract: R-spondin (Rspo) encodes a multi-domain protein that modulates the Wnt-signaling pathway. Two distinct rspo2 zebrafish mutants were generated by TALEN-mediated mutagenesis: a null mutant, rspo2(null), lacking all functional domains, and a hypomorphic mutant, rspo2(tsp), lacking the two N-terminal domains. Mutants were analyzed mainly for abnormalities in the skeletal system. Fin ray skeletons were formed normally in the rspo2(tsp) mutants, but were absent from the rspo2(null) mutants. Hypoplasia of the neural/hemal arches and ribs was observed in both mutants. Thus, the two rspo2 mutants help to identify the functions of Rspo2 in skeletogenesis, as well as functional differences among multiple Rspo2 domains.
authors.affiliation: Graduate School of Agricultural Science, Tohoku University, Sendai 981-8555, Japan.
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Tatsumi
authors.affiliation: Graduate School of Agricultural Science, Tohoku University, Sendai 981-8555, Japan.
authors.forename: Moe
authors.initials: M
authors.lastname: Takeda
authors.affiliation: Center for Bioscience Research and Education, Utsunomiya University, Utsunomiya 321-8505, Japan.
authors.forename: Masaru
authors.initials: M
authors.lastname: Matsuda
authors.affiliation: Graduate School of Agricultural Science, Tohoku University, Sendai 981-8555, Japan. Electronic address: suzukitr@bios.tohoku.ac.jp.
authors.forename: Tohru
authors.initials: T
authors.lastname: Suzuki
authors.affiliation: Graduate School of Agricultural Science, Tohoku University, Sendai 981-8555, Japan. Electronic address: hayokoi@bios.tohoku.ac.jp.
authors.forename: Hayato
authors.initials: H
authors.lastname: Yokoi
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D004262:DNA Restriction Enzymes / Q000378:metabolism*; D005786:Gene Expression Regulation; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D008969:Molecular Sequence Data; D016296:Mutagenesis; D009154:Mutation; D017434:Protein Structure, Tertiary; D012860:Skates, Fish / Q000002:abnormalities / Q000235:genetics* / Q000254:growth & development*; D015027:Zebrafish / Q000235:genetics*; D029961:Zebrafish Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D000098907:R-Spondins
chemical_list: D004262:DNA Restriction Enzymes; D036341:Intercellular Signaling Peptides and Proteins; D029961:Zebrafish Proteins; C000595220:Rspo2 protein, zebrafish; D000098907:R-Spondins
keywords: Fin ray; Mutagenesis; R-spondin2; Skeletogenesis; TALEN; Zebrafish