title: Modulation of viability and apoptosis of UVB-exposed human keratinocyte HaCaT cells by aqueous methanol extract of laver (Porphyra yezoensis).
abstract: We investigated the effect of 80% methanol extract of laver (Porphyra yezoensis) on the UVB-exposed HaCaT cells, human keratinocytes. The laver extract showed absorbance spectrum characteristic of porphyra-334 or shinorine, major mycosporine-like amino acids (MAAs) in red algae, and contained phenolic compounds. UVB exposure decreased cell viability and increased apoptotic cell fractions, and it also decreased the ratio of reduced (GSH) to oxidized glutathione (GSSG) and the total glutathione content. Post-treatment with the laver extract significantly increased the net viability and also the apoptotic cell fractions of UVB-exposed cells. The extract caused increase in GSH/GSSG ratio, yet it exacerbated the decrease in glutathione content in the UVB-exposed cells. These effects of the laver extract were also manifested in the sham-exposed cells, suggesting that those effects might be general phenomena caused by the laver extract. The extract treatment enhanced the UVB-induced phosphorylation of JNK and ERK, affecting more the latter. Our results suggest that the post-treatment with laver extract may protect UVB-exposed skin cells not only by increasing overall cell proliferation but also by enhancing apoptosis of damaged cells, via activating JNK and ERK signaling pathways, in which modulation of the content and redox status of glutathione may take significant parts.
authors.affiliation: Department of Chemistry, Incheon National University, Incheon 406-772, Republic of Korea.
authors.forename: Saerong
authors.initials: S
authors.lastname: Kim
authors.affiliation: Department of Cosmetic Science and Management, Incheon National University, Incheon 406-772, Republic of Korea.
authors.forename: Dong Hun
authors.initials: DH
authors.lastname: You
authors.affiliation: Department of Cosmetic Science and Management, Incheon National University, Incheon 406-772, Republic of Korea; Department of Marine Sciences, Incheon National University, Incheon 406-772, Republic of Korea.
authors.forename: Taejun
authors.initials: T
authors.lastname: Han
authors.affiliation: Department of Chemistry, Incheon National University, Incheon 406-772, Republic of Korea; Department of Cosmetic Science and Management, Incheon National University, Incheon 406-772, Republic of Korea. Electronic address: eunmi@incheon.ac.kr.
authors.forename: Eun-Mi
authors.initials: EM
authors.lastname: Choi
mesh_terms: D017209:Apoptosis / Q000187:drug effects* / Q000528:radiation effects; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects / Q000528:radiation effects; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D003512:Cyclohexanones / Q000737:chemistry / Q000494:pharmacology; D003514:Cyclohexylamines / Q000737:chemistry / Q000494:pharmacology; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D005978:Glutathione / Q000737:chemistry / Q000378:metabolism; D005998:Glycine / Q000031:analogs & derivatives / Q000737:chemistry / Q000494:pharmacology; D006801:Humans; D048031:JNK Mitogen-Activated Protein Kinases / Q000378:metabolism; D015603:Keratinocytes / Q000166:cytology / Q000187:drug effects / Q000528:radiation effects; D000432:Methanol / Q000737:chemistry; D010766:Phosphorylation / Q000187:drug effects / Q000528:radiation effects; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D044664:Porphyra / Q000737:chemistry* / Q000378:metabolism; D014466:Ultraviolet Rays
chemical_list: D003512:Cyclohexanones; D003514:Cyclohexylamines; D010936:Plant Extracts; C421025:porphyra-334; C494891:shinorine; D048049:Extracellular Signal-Regulated MAP Kinases; D048031:JNK Mitogen-Activated Protein Kinases; D005978:Glutathione; D005998:Glycine; D000432:Methanol