title: Blastocysts can be rebiopsied for preimplantation genetic diagnosis and screening.
abstract: OBJECTIVE
  To evaluate the clinical value of re-examining the test-failure blastocysts in preimplantation genetic diagnosis/screening cycles.
  
  
  METHODS
  Retrospective study.
  
  
  METHODS
  University-affiliated center.
  
  
  METHODS
  Women with test-failure blastocysts cryopreserved in preimplantation genetic diagnosis/screening cycles.
  
  
  METHODS
  Cryopreserved test-failure blastocysts were warmed and underwent a second round of biopsy, single nucleotide polymorphism microarray analysis, and vitrification, and the normal blastocysts were warmed again for ET.
  
  
  METHODS
  The percentage of test-failure blastocysts for transfer, the implantation rate per transferred blastocyst, and the live birth rate.
  
  
  RESULTS
  A total of 106 test-failure blastocysts from 77 cycles were warmed for re-examination. A total of 73 blastocysts that completely expanded were considered to have survived the warming process and were successfully rebiopsied. After single nucleotide polymorphism array analysis, 70 blastocysts yielded whole genome amplification product, and 31 had normal chromosomes (44.3%). A total of 19 normal blastocysts were warmed for ET, of which 18 survived and were transferred. The clinical pregnancy rate (implantation rate) was 50.0% in 10 single blastocyst transfer cycles, and all the implanted blastocysts resulted in healthy live births.
  
  
  CONCLUSIONS
  Test-failure blastocysts that survived from the first warming procedure can tolerate a second round of biopsy, vitrification, and warming, have a high chance of having normal chromosomes, and are worth being re-examined.
authors.affiliation: Institute of Reproductive and Stem Cell Engineering, Central South University, Changsha, People's Republic of China; Key Laboratory of Reproductive and Stem Cell Engineering, Ministry of Health, People's Republic of China; Reproductive and Genetic Hospital of CITIC-Xiangya, Changsha, People's Republic of China.
authors.forename: Shuoping
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Institute of Reproductive and Stem Cell Engineering, Central South University, Changsha, People's Republic of China; National Engineering and Research Center of Human Stem Cells, Changsha, People's Republic of China.
authors.forename: Ke
authors.initials: K
authors.lastname: Tan
authors.affiliation: Institute of Reproductive and Stem Cell Engineering, Central South University, Changsha, People's Republic of China; Key Laboratory of Reproductive and Stem Cell Engineering, Ministry of Health, People's Republic of China; Reproductive and Genetic Hospital of CITIC-Xiangya, Changsha, People's Republic of China.
authors.forename: Fei
authors.initials: F
authors.lastname: Gong
authors.affiliation: Institute of Reproductive and Stem Cell Engineering, Central South University, Changsha, People's Republic of China; Key Laboratory of Reproductive and Stem Cell Engineering, Ministry of Health, People's Republic of China; Reproductive and Genetic Hospital of CITIC-Xiangya, Changsha, People's Republic of China.
authors.forename: Yifan
authors.initials: Y
authors.lastname: Gu
authors.affiliation: Institute of Reproductive and Stem Cell Engineering, Central South University, Changsha, People's Republic of China; Key Laboratory of Reproductive and Stem Cell Engineering, Ministry of Health, People's Republic of China; Reproductive and Genetic Hospital of CITIC-Xiangya, Changsha, People's Republic of China.
authors.forename: Yueqiu
authors.initials: Y
authors.lastname: Tan
authors.affiliation: Institute of Reproductive and Stem Cell Engineering, Central South University, Changsha, People's Republic of China; Key Laboratory of Reproductive and Stem Cell Engineering, Ministry of Health, People's Republic of China; Reproductive and Genetic Hospital of CITIC-Xiangya, Changsha, People's Republic of China.
authors.forename: Changfu
authors.initials: C
authors.lastname: Lu
authors.affiliation: Institute of Reproductive and Stem Cell Engineering, Central South University, Changsha, People's Republic of China; Key Laboratory of Reproductive and Stem Cell Engineering, Ministry of Health, People's Republic of China; Reproductive and Genetic Hospital of CITIC-Xiangya, Changsha, People's Republic of China.
authors.forename: Keli
authors.initials: K
authors.lastname: Luo
authors.affiliation: Institute of Reproductive and Stem Cell Engineering, Central South University, Changsha, People's Republic of China; Key Laboratory of Reproductive and Stem Cell Engineering, Ministry of Health, People's Republic of China; Reproductive and Genetic Hospital of CITIC-Xiangya, Changsha, People's Republic of China; National Engineering and Research Center of Human Stem Cells, Changsha, People's Republic of China.
authors.forename: Guangxiu
authors.initials: G
authors.lastname: Lu
authors.affiliation: Institute of Reproductive and Stem Cell Engineering, Central South University, Changsha, People's Republic of China; Key Laboratory of Reproductive and Stem Cell Engineering, Ministry of Health, People's Republic of China; Reproductive and Genetic Hospital of CITIC-Xiangya, Changsha, People's Republic of China; National Engineering and Research Center of Human Stem Cells, Changsha, People's Republic of China. Electronic address: linggf@hotmail.com.
authors.forename: Ge
authors.initials: G
authors.lastname: Lin
mesh_terms: D000328:Adult; D001706:Biopsy; D001755:Blastocyst / Q000166:cytology*; D015925:Cryopreservation / Q000379:methods; D010064:Embryo Implantation; D004624:Embryo Transfer / Q000379:methods*; D005260:Female; D006801:Humans; D011247:Pregnancy; D018873:Pregnancy Rate; D019836:Preimplantation Diagnosis / Q000379:methods*
keywords: SNP array; Test-failure; biopsy; blastocyst; vitrification