title: Evaluation of nystatin containing chitosan hydrogels as potential dual action bio-active restorative materials: in vitro approach.
abstract: Healing is a speciﬁc biological process related to the general phenomenon of growth and tissue regeneration and is a process generally affected by several systemic conditions or as detrimental side-effects of chemotherapy- and radiotherapy-induced inflammation of the oral mucosa. The objectives of this study is to evaluate the novel chitosan based functional drug delivery systems, which can be successfully incorporated into "dual action bioactive restorative materials", capable of inducing in vitro improved wound healing prototype and containing an antibiotic, such as nystatin, krill oil as an antioxidant and hydroxyapatite as a molecular bone scaffold, which is naturally present in bone and is reported to be successfully used in promoting bone integration when implanted as well as promoting healing. The hydrogels were prepared using a protocol as previously reported by us. The physico-chemical features, including surface morphology (SEM), release behaviors, stability of the therapeutic agent-antioxidant-chitosan, were measured and compared to the earlier reported chitosan-antioxidant containing hydrogels. Structural investigations of the reactive surface of the hydrogel are reported. Release of nystatin was investigated for all newly prepared hydrogels. Bio-adhesive studies were performed in order to assess the suitability of these designer materials. Free radical defense capacity of the biomaterials was evaluated using established in vitro model. The bio-adhesive capacity of the materials in the in vitro system was tested and quantified. It was found that the favorable synergistic effect of free radical built-in defense mechanism of the new functional materials increased sustainable bio-adhesion and therefore acted as a functional multi-dimensional restorative material with potential application in wound healing in vitro.
authors.affiliation: Research and Development Department, VTPCHEM PTY LTD., Southport 4215, Australia. tamaraperchyonok@gmail.com.
authors.forename: V Tamara
authors.initials: VT
authors.lastname: Perchyonok
authors.affiliation: School of Dentistry and Oral Health, Gold Coast campus, Griffith University, QLD 4222, Australia.
authors.forename: Vanessa
authors.initials: V
authors.lastname: Reher
authors.affiliation: School of Material Science and Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, China.
authors.forename: Shengmiao
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Oral and Dental Research Institute, Faculty of Dentistry, University of the Western Cape, Private Bag X1, Tygerberg 7505, Cape Town, South Africa.
authors.forename: Nicki
authors.initials: N
authors.lastname: Basson
authors.affiliation: Oral and Dental Research Institute, Faculty of Dentistry, University of the Western Cape, Private Bag X1, Tygerberg 7505, Cape Town, South Africa.
authors.forename: Sias
authors.initials: S
authors.lastname: Grobler