title: Chemoenzymatic synthesis of lacto-N-tetrasaccharide and sialyl lacto-N-tetrasaccharides.
abstract: A concise and practical chemoenzymatic synthesis of lacto-N-tetraose, a major component and one of the most common core structures of human milk oligosaccharides (HMOs) was reported. This convergent synthesis relies on the glycosylation of a readily available lactoside acceptor with a lacto-N-biose donor generated from a highly efficient one-pot two-enzyme synthesis.
authors.affiliation: National Glycoengineering Research Center, School of Pharmaceutical Science, Shandong University, Jinan 250012, China.
authors.forename: Wenlong
authors.initials: W
authors.lastname: Yao
authors.affiliation: National Glycoengineering Research Center, School of Pharmaceutical Science, Shandong University, Jinan 250012, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Yan
authors.affiliation: Department of Chemistry, University of California, One Shields Avenue, Davis, CA 95616, USA.
authors.forename: Xi
authors.initials: X
authors.lastname: Chen
authors.affiliation: National Glycoengineering Research Center, School of Pharmaceutical Science, Shandong University, Jinan 250012, China; Key Laboratory of Chemical Biology (Ministry of Education), Shandong University, Jinan 250012, China.
authors.forename: Fengshan
authors.initials: F
authors.lastname: Wang
authors.affiliation: National Glycoengineering Research Center, School of Pharmaceutical Science, Shandong University, Jinan 250012, China. Electronic address: hzcao@sdu.edu.cn.
authors.forename: Hongzhi
authors.initials: H
authors.lastname: Cao
mesh_terms: D000117:Acetylglucosamine / Q000737:chemistry; D001644:Bifidobacterium / Q000201:enzymology; D060326:Chemistry Techniques, Synthetic; D048168:Escherichia coli K12 / Q000201:enzymology; D005686:Galactokinase / Q000378:metabolism*; D006031:Glycosylation; D006801:Humans; D009844:Oligosaccharides / Q000138:chemical synthesis* / Q000737:chemistry; D006005:Phosphorylases / Q000378:metabolism*
chemical_list: D009844:Oligosaccharides; C013084:lacto-N-neotetraose; D006005:Phosphorylases; D005686:Galactokinase; D000117:Acetylglucosamine
keywords: Chemoenzymatic synthesis; Glycosylation; Lacto-N-tetrasaccharide; Sialyl lacto-N-tetrasaccharide; Sialylation