title: Effect of choline carboxylate ionic liquids on biological membranes.
abstract: Choline carboxylates, ChCm, with m=2-10 and choline oleate are known as biocompatible substances, yet their influence on biological membranes is not well-known, and the effect on human skin has not previously been investigated. The short chain choline carboxylates ChCm with m=2, 4, 6 act as hydrotropes, solubilizing hydrophobic compounds in aqueous solution, while the longer chain choline carboxylates ChCm with m=8, 10 and oleate are able to form micelles. In the present study, the cytotoxicity of choline carboxylates was tested using HeLa and SK-MEL-28 cells. The influence of these substances on liposomes prepared from dipalmitoylphosphatidylcholine (DPPC) was also evaluated to provide insights on membrane interactions. It was observed that the choline carboxylates with a chain length of m>8 distinctly influence the bilayer, while the shorter ones had minimal interaction with the liposomes.
authors.affiliation: Institute of Physical and Theoretical Chemistry, University of Regensburg, Universitätsstrasse 31, D-93040 Regensburg, Germany.
authors.forename: Doris
authors.initials: D
authors.lastname: Rengstl
authors.affiliation: Institute of Pharmaceutical Biology, University of Regensburg, Universitätsstrasse 31, D-93040 Regensburg, Germany.
authors.forename: Birgit
authors.initials: B
authors.lastname: Kraus
authors.affiliation: Department of Mechanical Engineering and Engineering Sciences, University of North Carolina at Charlotte, 9201 University City Blvd., Charlotte, NC, 28223, United States of America.
authors.forename: Matthew
authors.initials: M
authors.lastname: Van Vorst
authors.affiliation: Department of Mechanical Engineering and Engineering Sciences, University of North Carolina at Charlotte, 9201 University City Blvd., Charlotte, NC, 28223, United States of America.
authors.forename: Gloria D
authors.initials: GD
authors.lastname: Elliott
authors.affiliation: Institute of Physical and Theoretical Chemistry, University of Regensburg, Universitätsstrasse 31, D-93040 Regensburg, Germany. Electronic address: Werner.Kunz@ur.de.
authors.forename: Werner
authors.initials: W
authors.lastname: Kunz
mesh_terms: D002151:Calorimetry; D002460:Cell Line; D002462:Cell Membrane / Q000187:drug effects*; D002794:Choline / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D052578:Ionic Liquids / Q000737:chemistry*; D008823:Micelles
chemical_list: D052578:Ionic Liquids; D008823:Micelles; D002794:Choline
keywords: Biological membrane; Choline ionic liquids; Cytotoxicity