title: Cloning and characterisation of the gene encoding 3-hydroxy-3-methylglutaryl-CoA synthase in Tripterygium wilfordii.
abstract: Tripterygium wilfordii is a traditional Chinese medical plant used to treat rheumatoid arthritis and cancer. The main bioactive compounds of the plant are diterpenoids and triterpenoids. 3-Hydroxy-3-methylglutaryl-CoA synthase (HMGS) catalyses the reaction of acetoacetyl-CoA to 3-hydroxy-3-methylglutaryl-CoA, which is the first committed enzyme in the mevalonate (MVA) pathway. The sequence information of HMGS in Tripterygium wilfordii is a basic resource necessary for studying the terpenoids in the plant. In this paper, full-length cDNA encoding HMGS was isolated from Tripterygium wilfordii (abbreviated TwHMGS, GenBank accession number: KM978213). The full length of TwHMGS is 1814 bp, and the gene encodes a protein with 465 amino acids. Sequence comparison revealed that TwHMGS exhibits high similarity to HMGSs of other plants. The tissue expression patterns revealed that the expression level of TwHMGS is highest in the stems and lowest in the roots. Induced expression of TwHMGS can be induced by MeJA, and the expression level is highest 4 h after induction. The functional complement assays in the YML126C knockout yeast demonstrated that TwHMGS participates in yeast terpenoid biosynthesis.
authors.affiliation: School of Traditional Chinese Medicine, Capital Medical University, Beijing 100069, China. lyjlife@sina.com.
authors.forename: Yu-Jia
authors.initials: YJ
authors.lastname: Liu
authors.affiliation: School of Traditional Chinese Medicine, Capital Medical University, Beijing 100069, China. diana1989119@sina.com.
authors.forename: Yu-Jun
authors.initials: YJ
authors.lastname: Zhao
authors.affiliation: School of Traditional Chinese Medicine, Capital Medical University, Beijing 100069, China. zhangmeng8846@163.com.
authors.forename: Meng
authors.initials: M
authors.lastname: Zhang
authors.affiliation: School of Traditional Chinese Medicine, Capital Medical University, Beijing 100069, China. suping120@163.com.
authors.forename: Ping
authors.initials: P
authors.lastname: Su
authors.affiliation: School of Traditional Chinese Medicine, Capital Medical University, Beijing 100069, China. wxj0517@sina.com.
authors.forename: Xiu-Juan
authors.initials: XJ
authors.lastname: Wang
authors.affiliation: School of Traditional Chinese Medicine, Capital Medical University, Beijing 100069, China. xnzhang@ccmu.edu.cn.
authors.forename: Xia-Nan
authors.initials: XN
authors.lastname: Zhang
authors.affiliation: School of Traditional Chinese Medicine, Capital Medical University, Beijing 100069, China. weigao@ccmu.edu.cn.
authors.forename: Wei
authors.initials: W
authors.lastname: Gao
authors.affiliation: National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China. huangluqi01@126.com.
authors.forename: Lu-Qi
authors.initials: LQ
authors.lastname: Huang
mesh_terms: D000085:Acetates / Q000494:pharmacology; D000595:Amino Acid Sequence; D055162:Biocatalysis / Q000187:drug effects; D003001:Cloning, Molecular; D003517:Cyclopentanes / Q000494:pharmacology; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D017343:Genes, Plant; D005816:Genetic Complementation Test; D006904:Hydroxymethylglutaryl-CoA Synthase / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D008958:Models, Molecular; D008969:Molecular Sequence Data; D054883:Oxylipins / Q000494:pharmacology; D010802:Phylogeny; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D029757:Tripterygium / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics*
chemical_list: D000085:Acetates; D003517:Cyclopentanes; D054883:Oxylipins; C072239:methyl jasmonate; D006904:Hydroxymethylglutaryl-CoA Synthase