title: Co-infection of methicillin-resistant Staphylococcus epidermidis, methicillin-resistant Staphylococcus aureus and extended spectrum β-lactamase producing Escherichia coli in bovine mastitis--three cases reported from India.
abstract: Emergence of antimicrobial resistance among bovine mastitis pathogens is the major cause of frequent therapeutic failure and a cause of concern for veterinary practitioners. This study describes intra-mammary infection of methicillin-resistant Staphylococcus epidermidis (MRSE), methicillin-resistant Staphylococcus aureus (MRSA) and extended spectrum β-lactamase (ESBL) producing Escherichia coli in two Holstein Friesian crossbred cows with subclinical mastitis and one non-descript cow with clinical mastitis in two different districts of West Bengal, India. In total, three MRSE, one MRSA and three ESBL producing E. coli were isolated from these cases. Both the crossbreds were detected with MRSE (HFSE1 and HFSE2) and ESBL producing E. coli (HFEC1 and HFEC2), whereas, simultaneous infection of three pathogens viz. MRSA (NDSA1), MRSE (NDSE1) and ESBL producing E. coli (NDEC1) was found in the non-descript cow. The methicillin-resistant isolates possessed mecA gene and exhibited resistance to various antibiotics such as amikacin, tetracycline and glycopeptides. The ESBL producers were positive for blaCTX-M and blaTEM genes; in addition, HFEC1 and HFEC2 were positive for blaSHV and possessed the genes for class I integron (int1), sulphonamide resistance (sul1), quinolone resistance (qnrS) and other virulence factors (papC, iucD and ESTA1). All the ESBL producers exhibited resistance to a variety of antibiotics tested including third- and fourth-generation cephalosporins and were also intermediately resistant to carbapenems. This is the first ever report on simultaneous occurrence of MRSE, MRSA and ESBL producing E. coli in bovine mastitis indicating a major concern for dairy industry and public health as well.
authors.affiliation: a Indian Veterinary Research Institute , Eastern Regional Station , 37 Belgachia Road, Kolkata - 700 037 , India.
authors.forename: Samiran
authors.initials: S
authors.lastname: Bandyopadhyay
authors.forename: Indranil
authors.initials: I
authors.lastname: Samanta
authors.forename: Debaraj
authors.initials: D
authors.lastname: Bhattacharyya
authors.forename: Pramod Kumar
authors.initials: PK
authors.lastname: Nanda
authors.forename: Debasish
authors.initials: D
authors.lastname: Kar
authors.forename: Jayanta
authors.initials: J
authors.lastname: Chowdhury
authors.forename: Premanshu
authors.initials: P
authors.lastname: Dandapat
authors.forename: Arun Kumar
authors.initials: AK
authors.lastname: Das
authors.forename: Nayan
authors.initials: N
authors.lastname: Batul
authors.forename: Bimalendu
authors.initials: B
authors.lastname: Mondal
authors.forename: Tapan Kumar
authors.initials: TK
authors.lastname: Dutta
authors.forename: Gunjan
authors.initials: G
authors.lastname: Das
authors.forename: Bikash Chandra
authors.initials: BC
authors.lastname: Das
authors.forename: Syamal
authors.initials: S
authors.lastname: Naskar
authors.forename: Uttam Kumar
authors.initials: UK
authors.lastname: Bandyopadhyay
authors.forename: Suresh Chandra
authors.initials: SC
authors.lastname: Das
authors.forename: Subhasish
authors.initials: S
authors.lastname: Bandyopadhyay
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002417:Cattle; D060085:Coinfection; D024901:Drug Resistance, Multiple, Bacterial; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics / Q000302:isolation & purification; D004927:Escherichia coli Infections / Q000150:complications / Q000382:microbiology / Q000662:veterinary*; D005260:Female; D007194:India; D008414:Mastitis, Bovine / Q000188:drug therapy / Q000382:microbiology*; D016106:Methicillin Resistance / Q000235:genetics; D055624:Methicillin-Resistant Staphylococcus aureus / Q000235:genetics / Q000302:isolation & purification; D008826:Microbial Sensitivity Tests; D013203:Staphylococcal Infections / Q000150:complications / Q000382:microbiology / Q000662:veterinary*; D013212:Staphylococcus epidermidis / Q000235:genetics / Q000302:isolation & purification; D001618:beta-Lactamases / Q000302:isolation & purification
chemical_list: D000900:Anti-Bacterial Agents; D001618:beta-Lactamases
keywords: ESBL; India; MRSA; MRSE; bovine; cow; mastitis