title: MiR-185 is involved in human breast carcinogenesis by targeting Vegfa.
abstract: MiR-185 expression has been associated with many cancers. However, the roles of miR-185 in human breast cancer remain elusive. Here, we found that miR-185 expression was decreased in human breast cancer tissues compared with healthy tissue controls. Up-regulation of miR-185 inhibited breast cancer cell proliferation and invasion and vice versa. MiR-185 was shown to bind to the 3′-untranslated region (UTR) of vascular endothelial growth factor a (Vegfa), and a significant inverse correlation was found between miR-185 and Vegfa. Vegfa overexpression partially restored the inhibition of cell proliferation and invasion that was induced by miR-185, and vice versa. Additionally, Vegfa expression was found to be high in human breast cancer tissues. Thus, miR-185-mediated Vegfa targeting may be involved in breast cancer formation.
authors.affiliation: Haidian Maternal & Child Health Hospital, Beijing, China; Reproductive and Genetic Center of National Research Institute for Family Planning, Beijing, China; Graduate School, Peking Union Medical College, Beijing, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Wang
authors.forename: Shi
authors.initials: S
authors.lastname: Tian
authors.forename: Hong-Bin
authors.initials: HB
authors.lastname: Wang
authors.forename: Da-Peng
authors.initials: DP
authors.lastname: Chu
authors.forename: Jing-Li
authors.initials: JL
authors.lastname: Cao
authors.forename: Hong-Fei
authors.initials: HF
authors.lastname: Xia
authors.forename: Xu
authors.initials: X
authors.lastname: Ma
mesh_terms: D017209:Apoptosis / Q000235:genetics; D014408:Biomarkers, Tumor / Q000235:genetics; D001943:Breast Neoplasms / Q000235:genetics* / Q000473:pathology*; D063646:Carcinogenesis / Q000235:genetics*; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation; D002470:Cell Survival / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics*
chemical_list: D014408:Biomarkers, Tumor; C552431:MIRN185 microRNA, human; D035683:MicroRNAs; D042461:Vascular Endothelial Growth Factor A