title: Genetic diversity and population structure of a Sichuan sika deer (Cervus sichuanicus) population in Tiebu Nature Reserve based on microsatellite variation.
abstract: Cervus sichuanicus is a species of sika deer (Cervus nippon Group). To date, research has mainly focused on quantity surveying and behavior studies, with genetic information on this species currently deficient. To provide scientific evidence to assist in the protection of this species, we collected Sichuan sika deer fecal samples from the Sichuan Tiebu Nature Reserve (TNR) and extracted DNA from those samples. Microsatellite loci of bovine were used for PCR amplification. After GeneScan, the genotype data were used to analyze the genetic diversity and population structure of the Sichuan sika deer in TNR. Results showed that the average expected heterozygosity of the Sichuan sika deer population in TNR was 0.562, equivalent to the average expected heterozygosity of endangered animals, such as Procapra przewalskii. Furthermore, 8 of 9 microsatellite loci significantly deviated from the Hardy-Weinberg equilibrium and two groups existed within the Sichuan sika deer TNR population. This genetic structure may be caused by a group of Manchurian sika deer (Cervus hortulorum) released in TNR.
authors.affiliation: School of Life Sciences, East China Normal University, Shanghai 200062, China;Shanghai Science & Technology Museum, 2000 Century Avenue, Shanghai 200127, China.
authors.forename: Ya
authors.initials: Y
authors.lastname: He
authors.affiliation: School of Life Sciences, East China Normal University, Shanghai 200062, China;
authors.forename: Zheng-Huan
authors.initials: ZH
authors.lastname: Wang
authors.affiliation: School of Life Sciences, East China Normal University, Shanghai 200062, China;Shanghai Science & Technology Museum, 2000 Century Avenue, Shanghai 200127, China . xmwang@ecnu.edu.cn.
authors.forename: Xiao-Ming
authors.initials: XM
authors.lastname: Wang
mesh_terms: D000818:Animals; D002681:China; D003670:Deer / Q000235:genetics*; D014644:Genetic Variation; D018895:Microsatellite Repeats / Q000235:genetics*
keywords: Genetic diversity; Microsatellite; Population structure; Sichuan sika deer