title: A new model of biodosimetry to integrate low and high doses.
abstract: Biological dosimetry, that is the estimation of the dose of an exposure to ionizing radiation by a biological parameter, is a very important tool in cases of radiation accidents. The score of dicentric chromosomes, considered to be the most accurate method for biological dosimetry, for low LET radiation and up to 5 Gy, fits very well to a linear-quadratic model of dose-effect curve assuming the Poisson distribution. The accuracy of this estimation raises difficulties for doses over 5 Gy, the highest dose of the majority of dose-effect curves used in biological dosimetry. At doses over 5 Gy most cells show difficulties in reaching mitosis and cannot be used to score dicentric chromosomes. In the present study with the treatment of lymphocyte cultures with caffeine and the standardization of the culture time, metaphases for doses up to 25 Gy have been analyzed. Here we present a new model for biological dosimetry, which includes a Gompertz-type function as the dose response, and also takes into account the underdispersion of aberration-among-cell distribution. The new model allows the estimation of doses of exposures to ionizing radiation of up to 25 Gy. Moreover, the model is more effective in estimating whole and partial body exposures than the classical method based on linear and linear-quadratic functions, suggesting their effectiveness and great potential to be used after high dose exposures of radiation.
authors.affiliation: Unitat d'Antropologia Biològica, Departament de Biologia Animal, Biologia Vegetal i Ecologia, Universitat Autònoma de Barcelona, Bellaterra, Spain.
authors.forename: Mònica
authors.initials: M
authors.lastname: Pujol
authors.affiliation: Unitat d'Antropologia Biològica, Departament de Biologia Animal, Biologia Vegetal i Ecologia, Universitat Autònoma de Barcelona, Bellaterra, Spain.
authors.forename: Joan-Francesc
authors.initials: JF
authors.lastname: Barquinero
authors.affiliation: Departament de Matemàtiques, Universitat Autònoma de Barcelona, Bellaterra, Spain.
authors.forename: Pedro
authors.initials: P
authors.lastname: Puig
authors.affiliation: Unitat d'Antropologia Biològica, Departament de Biologia Animal, Biologia Vegetal i Ecologia, Universitat Autònoma de Barcelona, Bellaterra, Spain.
authors.forename: Roser
authors.initials: R
authors.lastname: Puig
authors.affiliation: Unitat d'Antropologia Biològica, Departament de Biologia Animal, Biologia Vegetal i Ecologia, Universitat Autònoma de Barcelona, Bellaterra, Spain.
authors.forename: María Rosa
authors.initials: MR
authors.lastname: Caballín
authors.affiliation: Unitat de Biologia Cellular, Departament de Biologia Cel·lular, Fisiologia i Immunologia, Universitat Autònoma de Barcelona, Bellaterra, Spain.
authors.forename: Leonardo
authors.initials: L
authors.lastname: Barrios
mesh_terms: D000328:Adult; D002110:Caffeine / Q000008:administration & dosage; D004307:Dose-Response Relationship, Radiation; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D008962:Models, Theoretical; D011829:Radiation Dosage; D055815:Young Adult
chemical_list: D002110:Caffeine