title: Expanding the SRI domain family: a common scaffold for binding the phosphorylated C-terminal domain of RNA polymerase II.
abstract: The SRI domain is a small three-helix domain originally discovered near the C-terminus of both histone methyltransferase SETD2 and helicase RECQL5. The SRI domain binds to the C-terminal repeat domain of the largest subunit of RNA polymerase II, allowing SETD2 and RECQL5 to regulate various mechanisms associated with RNA transcription. Using original tools to detect common patterns in distantly related sequences, we have identified SRI domains in several additional proteins, most of which are involved in RNA metabolism. Combining sequence analysis with structural prediction, we show that this domain family is more diverse than previously thought and we predict critical structural and functional features.
authors.affiliation: CNRS UMR7590, Sorbonne Universités, Université Pierre et Marie Curie-Paris6 - MNHN - IRD - IUC, Paris, France.
authors.forename: Joseph
authors.initials: J
authors.lastname: Rebehmed
authors.affiliation: Laboratory of Genome Dynamics in the Immune System, INSERM UMR 1163, University Paris Descartes Sorbonne Paris Cité, Institut Imagine, Paris, France.
authors.forename: Patrick
authors.initials: P
authors.lastname: Revy
authors.affiliation: CNRS UMR7590, Sorbonne Universités, Université Pierre et Marie Curie-Paris6 - MNHN - IRD - IUC, Paris, France.
authors.forename: Guilhem
authors.initials: G
authors.lastname: Faure
authors.affiliation: Laboratory of Genome Dynamics in the Immune System, INSERM UMR 1163, University Paris Descartes Sorbonne Paris Cité, Institut Imagine, Paris, France.
authors.forename: Jean-Pierre
authors.initials: JP
authors.lastname: de Villartay
authors.affiliation: CNRS UMR7590, Sorbonne Universités, Université Pierre et Marie Curie-Paris6 - MNHN - IRD - IUC, Paris, France. Electronic address: isabelle.callebaut@impmc.upmc.fr.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Callebaut
mesh_terms: D000595:Amino Acid Sequence; D019295:Computational Biology; D006801:Humans; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010766:Phosphorylation; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D012319:RNA Polymerase II / Q000737:chemistry* / Q000378:metabolism*
chemical_list: D012319:RNA Polymerase II
keywords: FRIGIDA-ESSENTIAL 1; LSD1-like homolog 3; PHRF1; SCAF1; SCAF11; Sequence analysis