title: Analysis of a cytoskeleton-associated kinase PEAK1 and E-cadherin in gastric cancer.
abstract: The expression of pseudopodium-enriched atypical kinase 1(PEAK1) has been studied in human cancers. However, their roles in gastric cancer are still unknown. In this study, gastric cancer tissue microarrays were constructed with 159 gastric cancer tissue samples, 150 non-neoplastic gastric epithelium specimens and 152 lymph node samples. Immunohistochemical staining for PEAK1 and E-cadherin was performed. Our study found negative expression of PEAK1 in 113 of 159 (71.1%) gastric cancers, in 46 of 150 (30.7%) non-neoplastic gastric epithelium tissues and in 69 of 94 (73.4%) metastatic lymph nodes. Negative expression of PEAK1 and E-cadherin associated with tumor grading, depth of invasion, lymph node metastases, pTNM stage and macroscopic type. Patients with either positive PEAK1 or E-cadherin expression had a significantly higher survival than those with negative expression. When combined, PEAK1(-)/E-cadherin(-) had a significantly poor prognosis than the rest of the patients. The expression of PEAK1 protein was positively correlated with E-cadherin in cancer tissues. Cox regression analyses showed that PEAK1, E-cadherin and PEAK1(-)/E-cadherin(-) were independent predictors of overall survival. In conclusion, our findings suggest that loss of PEAK1 may play an important role in carcinogenesis and development of gastric cancer through activating epithelial to mesenchymal transition.
authors.affiliation: Department of Surgery, Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, PR China. Electronic address: quguoqing1973@163.com.
authors.forename: Qingqu
authors.initials: Q
authors.lastname: Guo
authors.affiliation: Department of Surgery, Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, PR China.
authors.forename: Wenjie
authors.initials: W
authors.lastname: Qin
authors.affiliation: Department of Oncosurgery, Anyang Tumor Hospital, Anyang, Henan Province, PR China.
authors.forename: Baozhong
authors.initials: B
authors.lastname: Li
authors.affiliation: Department of Pathology, Anyang Tumor Hospital, Anyang, Henan Province, PR China.
authors.forename: Haijun
authors.initials: H
authors.lastname: Yang
authors.affiliation: Department of Oncosurgery, Anyang Tumor Hospital, Anyang, Henan Province, PR China.
authors.forename: Jianyun
authors.initials: J
authors.lastname: Guan
authors.affiliation: Department of Oncosurgery, Anyang Tumor Hospital, Anyang, Henan Province, PR China.
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Department of Oncosurgery, Anyang Tumor Hospital, Anyang, Henan Province, PR China.
authors.forename: Shoumiao
authors.initials: S
authors.lastname: Li
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015703:Antigens, CD; D014408:Biomarkers, Tumor / Q000032:analysis*; D015820:Cadherins / Q000032:analysis*; D058750:Epithelial-Mesenchymal Transition; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D053208:Kaplan-Meier Estimate; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D015999:Multivariate Analysis; D060787:Neoplasm Grading; D009361:Neoplasm Invasiveness; D009367:Neoplasm Staging; D016016:Proportional Hazards Models; D011505:Protein-Tyrosine Kinases / Q000032:analysis*; D012307:Risk Factors; D013274:Stomach Neoplasms / Q000201:enzymology* / Q000401:mortality / Q000473:pathology / Q000601:surgery; D046888:Tissue Array Analysis
chemical_list: D015703:Antigens, CD; D014408:Biomarkers, Tumor; C513384:CDH1 protein, human; D015820:Cadherins; C573947:PEAK1 protein, human; D011505:Protein-Tyrosine Kinases
keywords: E-cadherin; Gastric cancer; Immunohistochemistry; PEAK1