title: Enhanced immune response of BMDCs pulsed with H9N2 AIV and CpG.
abstract: Dendritic cells (DCs), professional antigen presenting cells, have demonstrated effective in controlling the initial of innate immune, while CpG could improve the performance of immune system. To explore the mechanism of CpG enhancing the immune response, we compared different stimulated mouse DCs with systemic approach microarrays. Analysis revealed 1840 differentially expressed genes in H9N2 stimulated group, more than 1728 altered genes in inactive H9N2 group. Investigation also proved that CpG/inactive H9N2 co-stimulation changed 2140 genes, more than that in H9N2 group, strongly demonstrated that CpG improved the performance of inactive H9N2 vaccination. Pathways analysis founded that DCs response rapid to shift in their maturation state, which involved Toll-like receptor (TLR) pathway significantly. Microarrays results were also verified by qRT-PCR with 14 elected representative genes. Further analysis proved that co-stimulatory molecules (CD40, CD80, CD86 and MHC-II), regulatory protein (IRF-7 and TRAF-6) and pro-inflammatory cytokines (IL-1, IL-6 and IL-12) were all changed and involved in DCs maturation. At last we demonstrated TLR signalling pathway in chicken bone marrow-derived dendritic cells (chBM-DCs) stimulated with CpG. The distinct transcriptional profiles of DCs pulsed with various stimuli expanded our understanding of how DCs respond and recognize influenza.
authors.affiliation: Key Lab of Animal Physiology and Biochemistry, Ministry of Agriculture, Nanjing Agricultural University, China; Nanjing Agricultural University, Wei gang 1, Jiangsu, PR China.
authors.forename: Jian
authors.initials: J
authors.lastname: Lin
authors.affiliation: Key Lab of Animal Physiology and Biochemistry, Ministry of Agriculture, Nanjing Agricultural University, China; Nanjing Agricultural University, Wei gang 1, Jiangsu, PR China.
authors.forename: Yin Y
authors.initials: YY
authors.lastname: Yin
authors.affiliation: Key Lab of Animal Physiology and Biochemistry, Ministry of Agriculture, Nanjing Agricultural University, China; Nanjing Agricultural University, Wei gang 1, Jiangsu, PR China.
authors.forename: Tao
authors.initials: T
authors.lastname: Qin
authors.affiliation: Key Lab of Animal Physiology and Biochemistry, Ministry of Agriculture, Nanjing Agricultural University, China; Nanjing Agricultural University, Wei gang 1, Jiangsu, PR China.
authors.forename: Li Q
authors.initials: LQ
authors.lastname: Zhu
authors.affiliation: Key Lab of Animal Physiology and Biochemistry, Ministry of Agriculture, Nanjing Agricultural University, China; Nanjing Agricultural University, Wei gang 1, Jiangsu, PR China.
authors.forename: Qing H
authors.initials: QH
authors.lastname: Yu
authors.affiliation: Key Lab of Animal Physiology and Biochemistry, Ministry of Agriculture, Nanjing Agricultural University, China. Electronic address: zxbyq@njau.edu.cn.
authors.forename: Qian
authors.initials: Q
authors.lastname: Yang
mesh_terms: D000276:Adjuvants, Immunologic / Q000378:metabolism*; D000818:Animals; D003713:Dendritic Cells / Q000276:immunology* / Q000821:virology*; D020869:Gene Expression Profiling; D053127:Influenza A Virus, H9N2 Subtype / Q000276:immunology*; D007252:Influenza Vaccines / Q000276:immunology*; D008810:Mice, Inbred C57BL; D046228:Microarray Analysis; D009838:Oligodeoxyribonucleotides / Q000378:metabolism*; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D000276:Adjuvants, Immunologic; C408982:CPG-oligonucleotide; D007252:Influenza Vaccines; D009838:Oligodeoxyribonucleotides
keywords: Active or inactive H9N2; CpG; Dendritic cells; Immune responses; TLR signal pathway