title: Investigation of in vitro and in vivo antioxidant activities of flavonoids rich extract from the berries of Rhodomyrtus tomentosa(Ait.) Hassk.
abstract: This study investigated the in vitro and in vivo antioxidant activities of the flavonoids rich extract from Rhodomyrtus tomentosa Hassk (R. tomentosa) berries. The in vitro antioxidant assay demonstrated that the flavonoids rich extract (62.09% rutin equivalent) extracted by ethanol and purified by AB-8 macroporous resin was strong in reducing power, superoxide radical, hydroxyl radical and DPPH radical scavenging activity, as well as inhibiting lipid peroxidation. In the in vivo assays, the flavonoids rich extract significantly enhanced the activities of antioxidant enzymes in serums of mice after they were administered with the extract. The results suggested that the flavonoids rich extract from R. tomentosa fruits possesses potent antioxidant properties. In addition, the chemical compositions of flavonoids rich extract were identified by UPLC-TOF-MS/MS. Six flavonoids were tentatively identified as myricetin, quercetin, dihydromyricetin, kaempferol, quercetin 7,4'-diglucoside and vitexin. Therefore, R. tomentosa berries could be used as a new source of antioxidant ingredient.
authors.affiliation: College of Life Science, South China Normal University, Guangzhou 510631, PR China.
authors.forename: Pingping
authors.initials: P
authors.lastname: Wu
authors.affiliation: College of Life Science, South China Normal University, Guangzhou 510631, PR China.
authors.forename: Guangzhi
authors.initials: G
authors.lastname: Ma
authors.affiliation: College of Life Science, South China Normal University, Guangzhou 510631, PR China.
authors.forename: Nianghui
authors.initials: N
authors.lastname: Li
authors.affiliation: Milne Fruit Products, Inc, 804 Bennett Avenue, Prosser, WA 99350, USA.
authors.forename: Qian
authors.initials: Q
authors.lastname: Deng
authors.affiliation: College of Life Science, South China Normal University, Guangzhou 510631, PR China.
authors.forename: Yanyan
authors.initials: Y
authors.lastname: Yin
authors.affiliation: College of Life Science, South China Normal University, Guangzhou 510631, PR China. Electronic address: qiangdoctor@126.com.
authors.forename: Ruqiang
authors.initials: R
authors.lastname: Huang
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D005419:Flavonoids / Q000032:analysis / Q000494:pharmacology*; D005638:Fruit / Q000737:chemistry; D066298:In Vitro Techniques; D008297:Male; D051379:Mice; D027822:Myrtaceae / Q000737:chemistry*; D010936:Plant Extracts / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; D005419:Flavonoids; D010936:Plant Extracts
keywords: Antioxidant activity; Flavonoids rich extract; Rhodomyrtus tomentosa berries; UPLC–TOF-MS/MS