title: An aptamer based surface plasmon resonance biosensor for the detection of ochratoxin A in wine and peanut oil.
abstract: Ochratoxin A (OTA), as a kind of chlorophenolic mycotoxin, exist widely in plant origin food and is harmful to human. Herein, a surface plasmon resonance (SPR) biosensor using an anti-OTA aptamer immobilized sensor chip was developed to measure ochratoxin A (OTA) quantificationally through a straightforward direct binding assay. The streptavidin protein as a crosslinker was immobilized onto the surface of a sensor chip and the biotin-aptamer was captured through streptavidin-biotin interaction. The biosensor exhibited a detection range from 0.094 to 100ng/mL (linear range from 0.094 to 10ng/mL) of OTA with a lower detection limit of 0.005ng/mL. Detection of OTA in wine and peanut oil was further performed in the SPR biosensor using simple liquid-liquid extraction for sample pretreatments. Recoveries of ochratoxin A from spiked samples ranged from 86.9% to 116.5% and coefficients of variation (CVs) ranged from 0.2% to 6.9%. The developed methods in our studies showed good analytical performances with limits of detection much lower than the maximum residue limit, as well as a good reproducibility and stability.
authors.affiliation: Beijing Key Laboratory of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029 China; Institute of Medicinal Biotechnology, Chinese Academy of Medical Science and Peking Union Medical College, Beijing 100050 China.
authors.forename: Zhiling
authors.initials: Z
authors.lastname: Zhu
authors.affiliation: Beijing Key Laboratory of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029 China; Institute of Medicinal Biotechnology, Chinese Academy of Medical Science and Peking Union Medical College, Beijing 100050 China.
authors.forename: Mengxue
authors.initials: M
authors.lastname: Feng
authors.affiliation: Institute of Medicinal Biotechnology, Chinese Academy of Medical Science and Peking Union Medical College, Beijing 100050 China.
authors.forename: Limin
authors.initials: L
authors.lastname: Zuo
authors.affiliation: State Key Laboratory of NBC Protection for Civilian, Beijing, China.
authors.forename: Zhentai
authors.initials: Z
authors.lastname: Zhu
authors.affiliation: State Key Laboratory of NBC Protection for Civilian, Beijing, China.
authors.forename: Fengwei
authors.initials: F
authors.lastname: Wang
authors.affiliation: Beijing Key Laboratory of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029 China.
authors.forename: Long
authors.initials: L
authors.lastname: Chen
authors.affiliation: Institute of Medicinal Biotechnology, Chinese Academy of Medical Science and Peking Union Medical College, Beijing 100050 China.
authors.forename: Jinghua
authors.initials: J
authors.lastname: Li
authors.affiliation: Institute of Medicinal Biotechnology, Chinese Academy of Medical Science and Peking Union Medical College, Beijing 100050 China. Electronic address: shanguangzhi@imb.pumc.edu.cn.
authors.forename: Guangzhi
authors.initials: G
authors.lastname: Shan
authors.affiliation: Beijing Key Laboratory of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029 China. Electronic address: luosz@mail.buct.edu.cn.
authors.forename: Shi-Zhong
authors.initials: SZ
authors.lastname: Luo
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry*; D006801:Humans; D057230:Limit of Detection; D009183:Mycotoxins / Q000032:analysis*; D009793:Ochratoxins / Q000032:analysis*; D000074241:Peanut Oil; D010938:Plant Oils / Q000032:analysis*; D020349:Surface Plasmon Resonance / Q000379:methods*; D014920:Wine / Q000032:analysis*
chemical_list: D052157:Aptamers, Nucleotide; D009183:Mycotoxins; D009793:Ochratoxins; D000074241:Peanut Oil; D010938:Plant Oils; C025589:ochratoxin A
keywords: Aptamer; OTA analysis; Peanut oil analysis; SPR; Wine analysis