title: Methionine and cystine double deprivation stress suppresses glioma proliferation via inducing ROS/autophagy.
abstract: Cancer cells are highly dependent on methionine and cystine (Met-Cys) for survival and proliferation. However, the molecular mechanism is not fully clear. The present study is to investigate the effects of Met-Cys deprivation on glioma cells proliferation. The results showed that Met-Cys double deprivation had synergistic action on elevating ROS level, decreased GSH level and inhibition of glioma cell proliferation. Moreover, both of them deprivation triggered autophagy of glioma cells both in vitro and in vivo. Importantly, Met-Cys double restriction diet inhibited growth of glioma. These results provided a new regulation mechanism of Met-Cys metabolism on affecting glioma cell proliferation, suggesting that targeting Met-Cys metabolism may be a potential strategy for glioma therapy.
authors.affiliation: Department of Neurosurgery, The First Affiliated Hospital of Harbin Medical University, No. 23 Youzheng Street, Nangang District, Harbin, Heilongjiang Province, 150001, People's Republic of China; Institute of Brain Science, Harbin Medical University, Harbin, Heilongjiang Province, People's Republic of China; Department of Pharmacology (State-Province Key Laboratories of Biomedicine- Pharmaceutics of China), Harbin Medical University, Harbin, Heilongjiang Province, People's Republic of China.
authors.forename: Huailei
authors.initials: H
authors.lastname: Liu
authors.affiliation: Department of Neurosurgery, The First Affiliated Hospital of Harbin Medical University, No. 23 Youzheng Street, Nangang District, Harbin, Heilongjiang Province, 150001, People's Republic of China; Institute of Brain Science, Harbin Medical University, Harbin, Heilongjiang Province, People's Republic of China.
authors.forename: Weiguang
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Department of Neurosurgery, The First Affiliated Hospital of Harbin Medical University, No. 23 Youzheng Street, Nangang District, Harbin, Heilongjiang Province, 150001, People's Republic of China; Institute of Brain Science, Harbin Medical University, Harbin, Heilongjiang Province, People's Republic of China.
authors.forename: Kaikai
authors.initials: K
authors.lastname: Wang
authors.affiliation: Department of Neurosurgery, The First Affiliated Hospital of Harbin Medical University, No. 23 Youzheng Street, Nangang District, Harbin, Heilongjiang Province, 150001, People's Republic of China; Institute of Brain Science, Harbin Medical University, Harbin, Heilongjiang Province, People's Republic of China.
authors.forename: Xiaoxiong
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Neurosurgery, The First Affiliated Hospital of Harbin Medical University, No. 23 Youzheng Street, Nangang District, Harbin, Heilongjiang Province, 150001, People's Republic of China; Institute of Brain Science, Harbin Medical University, Harbin, Heilongjiang Province, People's Republic of China.
authors.forename: Fei
authors.initials: F
authors.lastname: Yin
authors.affiliation: Department of Neurosurgery, The First Affiliated Hospital of Harbin Medical University, No. 23 Youzheng Street, Nangang District, Harbin, Heilongjiang Province, 150001, People's Republic of China; Institute of Brain Science, Harbin Medical University, Harbin, Heilongjiang Province, People's Republic of China.
authors.forename: Chenguang
authors.initials: C
authors.lastname: Li
authors.affiliation: Department of Neurosurgery, The First Affiliated Hospital of Harbin Medical University, No. 23 Youzheng Street, Nangang District, Harbin, Heilongjiang Province, 150001, People's Republic of China; Institute of Brain Science, Harbin Medical University, Harbin, Heilongjiang Province, People's Republic of China.
authors.forename: Chunlei
authors.initials: C
authors.lastname: Wang
authors.affiliation: Department of Neurosurgery, The First Affiliated Hospital of Harbin Medical University, No. 23 Youzheng Street, Nangang District, Harbin, Heilongjiang Province, 150001, People's Republic of China; Institute of Brain Science, Harbin Medical University, Harbin, Heilongjiang Province, People's Republic of China.
authors.forename: Boxian
authors.initials: B
authors.lastname: Zhao
authors.affiliation: Department of Neurosurgery, The First Affiliated Hospital of Harbin Medical University, No. 23 Youzheng Street, Nangang District, Harbin, Heilongjiang Province, 150001, People's Republic of China; Institute of Brain Science, Harbin Medical University, Harbin, Heilongjiang Province, People's Republic of China.
authors.forename: Chen
authors.initials: C
authors.lastname: Zhong
authors.affiliation: Department of Neurosurgery, The First Affiliated Hospital of Harbin Medical University, No. 23 Youzheng Street, Nangang District, Harbin, Heilongjiang Province, 150001, People's Republic of China; Institute of Brain Science, Harbin Medical University, Harbin, Heilongjiang Province, People's Republic of China.
authors.forename: Jiakang
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Neurosurgery, The First Affiliated Hospital of Harbin Medical University, No. 23 Youzheng Street, Nangang District, Harbin, Heilongjiang Province, 150001, People's Republic of China; Institute of Brain Science, Harbin Medical University, Harbin, Heilongjiang Province, People's Republic of China.
authors.forename: Fei
authors.initials: F
authors.lastname: Peng
authors.affiliation: Department of Neurosurgery, The First Affiliated Hospital of Harbin Medical University, No. 23 Youzheng Street, Nangang District, Harbin, Heilongjiang Province, 150001, People's Republic of China; Institute of Brain Science, Harbin Medical University, Harbin, Heilongjiang Province, People's Republic of China.
authors.forename: Yunke
authors.initials: Y
authors.lastname: Bi
authors.affiliation: Department of Neurosurgery, The First Affiliated Hospital of Harbin Medical University, No. 23 Youzheng Street, Nangang District, Harbin, Heilongjiang Province, 150001, People's Republic of China; Institute of Brain Science, Harbin Medical University, Harbin, Heilongjiang Province, People's Republic of China.
authors.forename: Chen
authors.initials: C
authors.lastname: Shen
authors.affiliation: Department of Neurosurgery, The First Affiliated Hospital of Harbin Medical University, No. 23 Youzheng Street, Nangang District, Harbin, Heilongjiang Province, 150001, People's Republic of China; Institute of Brain Science, Harbin Medical University, Harbin, Heilongjiang Province, People's Republic of China.
authors.forename: Xu
authors.initials: X
authors.lastname: Hou
authors.affiliation: Department of Neurosurgery, The First Affiliated Hospital of Harbin Medical University, No. 23 Youzheng Street, Nangang District, Harbin, Heilongjiang Province, 150001, People's Republic of China; Institute of Brain Science, Harbin Medical University, Harbin, Heilongjiang Province, People's Republic of China.
authors.forename: Daming
authors.initials: D
authors.lastname: Zhang
authors.affiliation: Department of Neurosurgery, The First Affiliated Hospital of Harbin Medical University, No. 23 Youzheng Street, Nangang District, Harbin, Heilongjiang Province, 150001, People's Republic of China; Institute of Brain Science, Harbin Medical University, Harbin, Heilongjiang Province, People's Republic of China.
authors.forename: Yaohua
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Pharmacology (State-Province Key Laboratories of Biomedicine- Pharmaceutics of China), Harbin Medical University, Harbin, Heilongjiang Province, People's Republic of China.
authors.forename: Jing
authors.initials: J
authors.lastname: Ai
authors.affiliation: Department of Neurosurgery, The First Affiliated Hospital of Harbin Medical University, No. 23 Youzheng Street, Nangang District, Harbin, Heilongjiang Province, 150001, People's Republic of China; Institute of Brain Science, Harbin Medical University, Harbin, Heilongjiang Province, People's Republic of China. Electronic address: guangsz@hotmail.com.
authors.forename: Shiguang
authors.initials: S
authors.lastname: Zhao
mesh_terms: D000818:Animals; D001343:Autophagy / Q000187:drug effects*; D001932:Brain Neoplasms / Q000473:pathology*; D049109:Cell Proliferation; D003553:Cystine / Q000172:deficiency*; D005910:Glioma / Q000473:pathology*; D008715:Methionine / Q000172:deficiency*; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D017382:Reactive Oxygen Species / Q000378:metabolism*
chemical_list: D017382:Reactive Oxygen Species; D003553:Cystine; D008715:Methionine
keywords: Autophagy; Cystine/cysteine; Glioma; Methionine; ROS