title: Gypenosides protected the neural stem cells in the subventricular zone of neonatal rats that were prenatally exposed to ethanol.
abstract: Fetal alcohol spectrum disorder (FASD) can cause severe mental retardation in children who are prenatally exposed to ethanol. The effects of prenatal and early postnatal ethanol exposure on adult hippocampal neurogenesis have been investigated; however, the effects of prenatal ethanol exposure on the subventricular zone (SVZ) have not. Gypenosides (GPs) have been reported to have neuroprotective effects in addition to other bioactivities. The effects of GPs on neural stem cells (NSCs) in the FASD model are unknown. Here, we test the effect of prenatal ethanol exposure on the neonatal SVZ, and the protection potential of GPs on NSCs in FASD rats. Our results show that prenatal ethanol exposure can suppress the cell proliferation and differentiation of neural stem cells in the neonatal SVZ and that GPs (400 mg/kg/day) can significantly increase the cell proliferation and differentiation of neural stem cells inhibited by ethanol. Our data indicate that GPs have neuroprotective effects on the NSCs and can enhance the neurogenesis inhibited by ethanol within the SVZ of neonatal rats. These findings provide new evidence for a potential therapy involving GPs for the treatment of FASD.
authors.affiliation: Department of Cell Biology, School of Medicine, Shandong University, Jinan 250012, China. 13589030976@163.com.
authors.forename: Lun
authors.initials: L
authors.lastname: Dong
authors.affiliation: Department of Cell Biology, School of Medicine, Shandong University, Jinan 250012, China. kunqiyang@126.com.
authors.forename: Kun-Qi
authors.initials: KQ
authors.lastname: Yang
authors.affiliation: Department of Cell Biology, School of Medicine, Shandong University, Jinan 250012, China. fuwenyan411@163.com.
authors.forename: Wen-Yan
authors.initials: WY
authors.lastname: Fu
authors.affiliation: Department of Cell Biology, School of Medicine, Shandong University, Jinan 250012, China. shangzhenhua16@126.com.
authors.forename: Zhen-Hua
authors.initials: ZH
authors.lastname: Shang
authors.affiliation: Department of Cell Biology, School of Medicine, Shandong University, Jinan 250012, China. zqy2008512@163.com.
authors.forename: Qing-Yu
authors.initials: QY
authors.lastname: Zhang
authors.affiliation: Department of Cell Biology, School of Medicine, Shandong University, Jinan 250012, China. amber_jfm@163.com.
authors.forename: Fang-Miao
authors.initials: FM
authors.lastname: Jing
authors.affiliation: Department of Cell Biology, School of Medicine, Shandong University, Jinan 250012, China. 13583108986@163.com.
authors.forename: Lin-Lin
authors.initials: LL
authors.lastname: Li
authors.affiliation: Department of Cell Biology, School of Medicine, Shandong University, Jinan 250012, China. xinhua@sdu.edu.cn.
authors.forename: Hua
authors.initials: H
authors.lastname: Xin
authors.affiliation: Department of Cell Biology, School of Medicine, Shandong University, Jinan 250012, China. luckingwang@sdu.edu.cn.
authors.forename: Xiao-Jing
authors.initials: XJ
authors.lastname: Wang
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D002452:Cell Count; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D019610:Cytoprotection / Q000187:drug effects; D000089622:Doublecortin Domain Proteins; D004622:Embryo, Mammalian / Q000187:drug effects / Q000473:pathology; D000431:Ethanol / Q000097:blood / Q000633:toxicity*; D005260:Female; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D029774:Gynostemma; D020547:Lateral Ventricles / Q000473:pathology*; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D064231:Nestin / Q000378:metabolism; D058953:Neural Stem Cells / Q000187:drug effects / Q000378:metabolism / Q000473:pathology*; D009479:Neuropeptides / Q000378:metabolism; D018696:Neuroprotective Agents / Q000494:pharmacology*; D010936:Plant Extracts / Q000494:pharmacology; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects / Q000473:pathology*; D017208:Rats, Wistar
chemical_list: D000089622:Doublecortin Domain Proteins; D005904:Glial Fibrillary Acidic Protein; D008869:Microtubule-Associated Proteins; D064231:Nestin; D009479:Neuropeptides; D018696:Neuroprotective Agents; D010936:Plant Extracts; C474918:gypenoside; D000431:Ethanol