title: Effect of water-soluble chitosan on the osteoblast function in MC3T3-E1 cells.
abstract: In the present study, water-soluble chitosan (WSC) was prepared by hydrolysis of chitosan with commercial α-amylase, and the effect of WSC on osteoblast function, i.e. alkaline phosphatase activity (ALP), cell viability, mineralization, and reactive oxygen species (ROS) in osteoblastic MC3T3-E1 cells was investigated. Osteoblastic MC3T3-E1 cells were cultured in various concentrations of WSC solutions (1-5 mg/mL) for designated time and then the ALP, cell viability, mineralization, and ROS in the cells were evaluated. Treatments of osteoblastic with WSC caused a significant increase in ALP, cell viability, and mineralization in osteoblastic MC3T3-E1 cells. Moreover, treatments of osteoblastic with WSC decreased ROS level in osteoblastic MC3T3-E1 cells. The results demonstrate that the WSC may reduce or prevent osteoblasts degeneration through antioxidant activity.
authors.affiliation: Department of Geriatrics, The Second People's Hospital of Lianyungang, 41 East Hailian Road, Xinpu 222006, China; Department of Electronic and Information Engineering Center, The Second People's Hospital of Lianyungang, 41 East Hailian Road, Xinpu 222006, China; Department of Clinical Science Experiment Center, The Second People's Hospital of Lianyungang, 41 East Hailian Road, Xinpu 222006, China.
authors.forename: Jian-Wen
authors.initials: JW
authors.lastname: Hou
authors.affiliation: Department of Microbiology Laboratory, The Second People's Hospital of Lianyungang, 41 East Hailian Road, Xinpu 222006, China; Department of Electronic and Information Engineering Center, The Second People's Hospital of Lianyungang, 41 East Hailian Road, Xinpu 222006, China; Department of Clinical Science Experiment Center, The Second People's Hospital of Lianyungang, 41 East Hailian Road, Xinpu 222006, China. Electronic address: qianlilyg@163.com.
authors.forename: Li
authors.initials: L
authors.lastname: Qian
authors.affiliation: Department of Geriatrics, The Second People's Hospital of Lianyungang, 41 East Hailian Road, Xinpu 222006, China; Department of Electronic and Information Engineering Center, The Second People's Hospital of Lianyungang, 41 East Hailian Road, Xinpu 222006, China; Department of Clinical Science Experiment Center, The Second People's Hospital of Lianyungang, 41 East Hailian Road, Xinpu 222006, China.
authors.forename: Jian-Ming
authors.initials: JM
authors.lastname: Kou
authors.affiliation: Department of Geriatrics, The Second People's Hospital of Lianyungang, 41 East Hailian Road, Xinpu 222006, China; Department of Electronic and Information Engineering Center, The Second People's Hospital of Lianyungang, 41 East Hailian Road, Xinpu 222006, China; Department of Clinical Science Experiment Center, The Second People's Hospital of Lianyungang, 41 East Hailian Road, Xinpu 222006, China.
authors.forename: Cui-Wen
authors.initials: CW
authors.lastname: Zhang
authors.affiliation: Department of Geriatrics, The Second People's Hospital of Lianyungang, 41 East Hailian Road, Xinpu 222006, China; Department of Electronic and Information Engineering Center, The Second People's Hospital of Lianyungang, 41 East Hailian Road, Xinpu 222006, China; Department of Clinical Science Experiment Center, The Second People's Hospital of Lianyungang, 41 East Hailian Road, Xinpu 222006, China.
authors.forename: Xue-Jun
authors.initials: XJ
authors.lastname: Jia
authors.affiliation: Department of Geriatrics, The Second People's Hospital of Lianyungang, 41 East Hailian Road, Xinpu 222006, China; Department of Electronic and Information Engineering Center, The Second People's Hospital of Lianyungang, 41 East Hailian Road, Xinpu 222006, China; Department of Clinical Science Experiment Center, The Second People's Hospital of Lianyungang, 41 East Hailian Road, Xinpu 222006, China. Electronic address: tinasxz@yahoo.com.cn.
authors.forename: Wei
authors.initials: W
authors.lastname: Tian
mesh_terms: D000469:Alkaline Phosphatase / Q000096:biosynthesis; D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage* / Q000737:chemistry; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D048271:Chitosan / Q000008:administration & dosage* / Q000737:chemistry; D051379:Mice; D010006:Osteoblasts / Q000187:drug effects*; D017382:Reactive Oxygen Species / Q000378:metabolism; D012995:Solubility; D014867:Water / Q000737:chemistry
chemical_list: D000975:Antioxidants; D017382:Reactive Oxygen Species; D014867:Water; D048271:Chitosan; D000469:Alkaline Phosphatase
keywords: Chitosa; Osteoblast