title: [Determination of hexabromocyclododecanes diastereoisomers in human breast milk by HPLC-MS/MS].
abstract: OBJECTIVE
  A method for the determination of ac, 3 and γ-hexabromocyclododecanes (HBCDs) in human breast milk was developed by HPLC-MS/MS.
  
  
  METHODS
  3 -5 g human breast milk powder was spiked with '3C-HBCDs and then been extracted using Soxhlet extraction. The extract was dried and dissolved in 6ml of cyclohexane/ethyl acetate (1:1 ), then purified by gel permeation chromatography (GPC). The effluent was concentrated with rotary evaporation and then re-dissolved in hexane. 2ml of sulphuric acid was added to remove the fat for further clean-up. After drying under nitrogen, the supernatant was dissolved in 100 µl of methanol and finally determined by HPLC-MS/MS.
  
  
  RESULTS
  The linear range for the three diastereoisomers of HBCDs was in 1 - 50 µg/L, with correlation coefficients ranging from 0. 9997 to 0.998. The detection limits of the three diastereoisomers ranged from 0. 12 to 0. 22 µg/L. The recoveries for three spiking levels ranged from 82. 80% to 110. 60% . The intra-day and inter-day relative standard deviations (RSD) were all less than 9. 4%.
  
  
  CONCLUSIONS
  The developed method was simple, convenient and sensitive. It was suitable for the determination of or, P3 and y-HBCDs in breast milk and other matrix in the future.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Tan
authors.forename: Shaoyou
authors.initials: S
authors.lastname: Lu
authors.forename: Jianqing
authors.initials: J
authors.lastname: Zhang
authors.forename: Yousheng
authors.initials: Y
authors.lastname: Jiang
authors.forename: Jian
authors.initials: J
authors.lastname: Zhou
authors.forename: Zhou
authors.initials: Z
authors.lastname: Zhu
authors.forename: Honghe
authors.initials: H
authors.lastname: Liu
authors.forename: Shengnong
authors.initials: S
authors.lastname: Li
authors.forename: Xiaoshi
authors.initials: X
authors.lastname: Lin
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005260:Female; D005504:Food Analysis / Q000379:methods*; D006801:Humans; D006842:Hydrocarbons, Brominated; D008895:Milk, Human / Q000737:chemistry*; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D006842:Hydrocarbons, Brominated; C089796:hexabromocyclododecane