title: Insulin-independent actions of glucagon-like peptide-1 in wethers.
abstract: Insulin-independent actions of glucagon-like peptide-1 (GLP-1) are not yet clear in ruminants. Four Suffolk mature wethers (60.0 ± 6.7 kg body weight (BW)) were intravenously infused with insulin (0.5 mU/kg BW/min; from 0 to 90 min) and GLP-1 (0.5 μg/kg BW/min; from 60 to 150 min) with both hormones co-administered from 60 to 90 min, in a repeated-measure design under euglycemic clamp for 150 min, to investigate whether GLP-1 has insulin-independent actions. Jugular blood samples were taken at 15-min intervals for plasma hormones and metabolites analysis. Compared to baseline concentrations (at 0 min), insulin infusion decreased (P < 0.05) plasma concentrations of glucagon, non-esterified fatty acids (NEFA), lactate, nonessential amino acids (NEAA), branched-chain amino acids (BCAA), total amino acids (TAA) and urea nitrogen (UN). Insulin plus GLP-1 infusion induced a greater increase (P < 0.05) in plasma concentrations of insulin and triglyceride (TG), but decreased (P < 0.05) glucagon, total cholesterol (T-Cho), NEAA and UN plasma concentrations. GLP-1 infusion increased (P < 0.05) NEFA, β-hydroxybutyrate and TG, but decreased (P < 0.05) glucagon, T-Cho, NEAA, BCAA and UN plasma concentrations. In conclusion, GLP-1 exerts extrapancreatic roles in ruminants not only insulin-independent but probably, in contrast to non-ruminants, antagonistic to insulin effects.
authors.affiliation: Graduate School of Biosphere Science, Hiroshima University, Higashi-Hiroshima, Japan; Faculty of Veterinary Medicine, Kafrelsheikh University, Kafrelsheikh, Egypt.
authors.forename: Mabrouk
authors.initials: M
authors.lastname: El-Sabagh
authors.forename: Dai
authors.initials: D
authors.lastname: Taniguchi
authors.forename: Toshihisa
authors.initials: T
authors.lastname: Sugino
authors.forename: Taketo
authors.initials: T
authors.lastname: Obitsu
authors.forename: Kohzo
authors.initials: K
authors.lastname: Taniguchi
mesh_terms: D000273:Adipose Tissue / Q000378:metabolism; D000597:Amino Acids, Branched-Chain / Q000097:blood; D000818:Animals; D001786:Blood Glucose; D001806:Blood Urea Nitrogen; D002784:Cholesterol / Q000097:blood; D005230:Fatty Acids, Nonesterified / Q000097:blood; D005934:Glucagon / Q000097:blood; D052216:Glucagon-Like Peptide 1 / Q000008:administration & dosage / Q000494:pharmacology / Q000502:physiology*; D006041:Goats / Q000378:metabolism*; D007262:Infusions, Intravenous; D007328:Insulin / Q000008:administration & dosage / Q000097:blood / Q000494:pharmacology / Q000502:physiology*; D007329:Insulin Antagonists; D019344:Lactic Acid / Q000097:blood; D050356:Lipid Metabolism; D008099:Liver / Q000378:metabolism; D008297:Male; D014280:Triglycerides / Q000097:blood
chemical_list: D000597:Amino Acids, Branched-Chain; D001786:Blood Glucose; D005230:Fatty Acids, Nonesterified; D007328:Insulin; D007329:Insulin Antagonists; D014280:Triglycerides; D019344:Lactic Acid; D052216:Glucagon-Like Peptide 1; D005934:Glucagon; D002784:Cholesterol
keywords: extrapancreatic effects; glucagon-like peptide-1 (7-36) amide; insulin; wethers