title: Effects of ubiquinone derivatives on the mitochondrial unselective channel of Saccharomyces cerevisiae.
abstract: Ubiquinone derivatives modulate the mammalian mitochondrial Permeability Transition Pore (PTP). Yeast mitochondria harbor a similar structure: the respiration- and ATP-induced Saccharomyces cerevisiae Mitochondrial Unselective Channel ( Sc MUC). Here we show that decylubiquinone, a well-characterized inhibitor of the PTP, suppresses Sc MUC opening in diverse strains and independently of respiratory chain modulation or redox-state. We also found that naturally occurring derivatives such as hexaprenyl and decaprenyl ubiquinones lacked effects on the Sc MUC. The PTP-inactive ubiquinone 5 (Ub5) promoted the Sc MUC-independent activation of the respiratory chain in most strains tested. In an industrial strain however, Ub5 blocked the protection elicited by dUb. The results indicate the presence of a ubiquinone-binding site in the Sc MUC.
authors.affiliation: Dalton Cardiovascular Research Center, University of Missouri-Columbia, 134 Research Park Dr, Columbia, MO, 65211, U.S.A, gutierrezaguilarm@missouri.edu.
authors.forename: Manuel
authors.initials: M
authors.lastname: Gutiérrez-Aguilar
authors.forename: Helga M
authors.initials: HM
authors.lastname: López-Carbajal
authors.forename: Cristina
authors.initials: C
authors.lastname: Uribe-Alvarez
authors.forename: Emilio
authors.initials: E
authors.lastname: Espinoza-Simón
authors.forename: Mónica
authors.initials: M
authors.lastname: Rosas-Lemus
authors.forename: Natalia
authors.initials: N
authors.lastname: Chiquete-Félix
authors.forename: Salvador
authors.initials: S
authors.lastname: Uribe-Carvajal
mesh_terms: D000818:Animals; D008928:Mitochondria / Q000378:metabolism*; D033681:Mitochondrial Membrane Transport Proteins / Q000378:metabolism*; D000083162:Mitochondrial Permeability Transition Pore; D017382:Reactive Oxygen Species; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D014451:Ubiquinone / Q000235:genetics* / Q000378:metabolism*; D015003:Yeasts
chemical_list: D033681:Mitochondrial Membrane Transport Proteins; D000083162:Mitochondrial Permeability Transition Pore; D017382:Reactive Oxygen Species; D014451:Ubiquinone; C025203:Ubiquinone Q1