title: Development of complete hydrolysis of pectins from apple pomace.
abstract: Enzymatically extracted pectins have a more complex structure than those obtained by conventional methods. As a result, they are less susceptible to hydrolysis, which makes the precise determination of their composition difficult. The aim of the study was to develop a method of complete hydrolysis of enzymatically extracted apple pectins. Substrates were pectins isolated from apple pomace by the use of xylanase and multicatalytic preparation Celluclast and apple pomace. Hydrolysis was performed by a chemical method with 2M TFA at 100 °C and 120 °C and a combined acidic/enzymatic method. After hydrolysis, the contents of galacturonic acid and neutral sugars were measured by HPLC. Complete hydrolysis of polygalacturonic acid occurred after 2.5h incubation with 2M TFA at 120 °C. The efficient hydrolysis of neutral sugars in pectins was performed with 2M TFA at 100 °C for 2.5h. Monomers most susceptible to concentrated acid were rhamnose, mannose and arabinose.
authors.affiliation: Department of Food Biotechnology, Faculty of Food Technology, Agricultural University of Cracow, 31-149 Kraków, ul. Balicka 122, Poland. Electronic address: a.wikiera@ur.krakow.pl.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Wikiera
authors.affiliation: Department of Food Biotechnology, Faculty of Food Technology, Agricultural University of Cracow, 31-149 Kraków, ul. Balicka 122, Poland.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Mika
authors.affiliation: Department of Food Biotechnology, Faculty of Food Technology, Agricultural University of Cracow, 31-149 Kraków, ul. Balicka 122, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Starzyńska-Janiszewska
authors.affiliation: Department of Food Biotechnology, Faculty of Food Technology, Agricultural University of Cracow, 31-149 Kraków, ul. Balicka 122, Poland.
authors.forename: Bożena
authors.initials: B
authors.lastname: Stodolak
mesh_terms: D001089:Arabinose / Q000378:metabolism; D002241:Carbohydrates / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D006603:Hexuronic Acids / Q000032:analysis; D006868:Hydrolysis; D027845:Malus / Q000737:chemistry*; D010368:Pectins / Q000032:analysis / Q000737:chemistry*
chemical_list: D002241:Carbohydrates; D006603:Hexuronic Acids; C007819:galacturonic acid; D010368:Pectins; D001089:Arabinose
keywords: Apple pectin; Chemical hydrolysis; Enzymatic hydrolysis; Galacturonic acid; Pectin neutral sugar