title: Characterization of two putative prolinases (PepR1 and PepR2) from Lactobacillus plantarum WCFS1: occurrence of two isozymes with structural similarity and different catalytic properties.
abstract: Two putative prolinases (PepR1 and PepR2) of Lactobacillus plantarum WCSF1 share 48.5% amino acid sequence identity (55.5% at the DNA level); however, PepR1 exhibits over 80% identity at the protein level with other lactobacilli prolinases while PepR2 exhibits only 51% or less identity. In this study, the putative genes were overexpressed in Escherichia coli, purified to gel electrophoretic homogeneity, and then characterized. Purified PepR1 and PepR2 hydrolysed Pro-Xaa dipeptide substrates at similar rates, proving their nature as prolinases. Structural analyses using circular dichroism, dynamic light scattering, gel filtration, and molecular modelling revealed that the two prolinases have similar structural characteristics: high β-sheet content, homotetrameric structure, and similar folding to the PepI/PepL/PepR peptidase family. However, kinetic and thermodynamic analyses of PepR1 and PepR2 indicated differences in many aspects: optimum temperatures (25 and 30°C, respectively), optimum pH (pH7.5 and 8.0, respectively), substrate specificities (high stringency of PepR2), kinetic parameters, and thermal stability (29 and 48°C, respectively). Also, these prolinases behaved differently towards inhibitor treatments, suggesting structural and/or functional differences in their active sites. Differences in the two prolinases would contribute to a diversity of catalytic activities, so that they work together cooperatively and complementarily to hydrolyse proline-containing peptides with broader specificity, working pH, working temperature, and higher efficiency, thus allowing adaptation to a wider range of environments.
authors.affiliation: Department of Food and Bioproduct Sciences, College of Agriculture and Bioresources, University of Saskatchewan, 51 Campus Dr., Saskatoon, SK S7N 5A8, Canada.
authors.forename: Yanyu
authors.initials: Y
authors.lastname: Huang
authors.affiliation: Department of Food and Bioproduct Sciences, College of Agriculture and Bioresources, University of Saskatchewan, 51 Campus Dr., Saskatoon, SK S7N 5A8, Canada. Electronic address: takuji.tanaka@usask.ca.
authors.forename: Takuji
authors.initials: T
authors.lastname: Tanaka
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics; D002384:Catalysis; D002942:Circular Dichroism; D004150:Dipeptidases / Q000737:chemistry*; D004151:Dipeptides / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D007527:Isoenzymes / Q000737:chemistry* / Q000235:genetics; D007700:Kinetics; D048191:Lactiplantibacillus plantarum / Q000201:enzymology*; D008958:Models, Molecular; D016415:Sequence Alignment; D013379:Substrate Specificity
chemical_list: D001426:Bacterial Proteins; D004151:Dipeptides; D007527:Isoenzymes; D004150:Dipeptidases; C000597421:PepR1 protein, Lactobacillus plantarum
keywords: Bitterness; Molecular cloning; PepI/PepL/PepR family; Prolidase; Proline