title: Electrochemical impediometric detection of anti-HIV drug taking gold nanorods as a sensing interface.
abstract: In present work, gold nanorods were used for amplification of electrochemical sensing of anti-HIV replication drug i.e. deferiprone. Gold nanorods (nano Au) deposited onto pencil graphite electrode (PGE) has been utilized for covalent immobilization of horse radish peroxidase (HRP), via glutaraldehyde (Glu), for deferiprone detection using impedimetric technique. Gold nanorods (nano Au) prepared were characterized by TEM and XRD. The resulting nano Au sensor exhibited a good response to deferiprone with a wide linear range (0.005-1000µM) and a low detection limit 0.005µM. The biosensor also showed a short response time (within 15s). In addition, the biosensor exhibited high reproducibility, good storage stability and anti-interference ability. The applicability of the nano Au sensor is to determine deferiprone level in spiked urine and serum samples.
authors.affiliation: Amity Institute of Nanotechnology, AMITY University, Noida, UP, India. Electronic address: jags_biotech@yahoo.co.in.
authors.forename: Jagriti
authors.initials: J
authors.lastname: Narang
authors.affiliation: Amity Institute of Physiotherapy, AMITY University, Noida, UP, India.
authors.forename: Nitesh
authors.initials: N
authors.lastname: Malhotra
authors.affiliation: Faculty of Pharmaceutical Science PGIMS, Rohtak, India.
authors.forename: Gajendra
authors.initials: G
authors.lastname: Singh
authors.affiliation: Department of Biochemistry, M. D. University, Rohtak 124001, Haryana, India.
authors.forename: C S
authors.initials: CS
authors.lastname: Pundir
mesh_terms: D019380:Anti-HIV Agents / Q000097:blood* / Q000652:urine*; D015374:Biosensing Techniques / Q000295:instrumentation; D000077543:Deferiprone; D055664:Electrochemical Techniques / Q000295:instrumentation*; D004800:Enzymes, Immobilized / Q000737:chemistry; D004867:Equipment Design; D006046:Gold / Q000737:chemistry*; D006735:Horseradish Peroxidase / Q000737:chemistry; D006801:Humans; D057230:Limit of Detection; D043942:Nanotubes / Q000737:chemistry* / Q000648:ultrastructure; D011728:Pyridones / Q000097:blood* / Q000652:urine*; D015203:Reproducibility of Results
chemical_list: D019380:Anti-HIV Agents; D004800:Enzymes, Immobilized; D011728:Pyridones; D000077543:Deferiprone; D006046:Gold; D006735:Horseradish Peroxidase
keywords: Biosensor; Deferiprone; Gold nanorods; Horse radish peroxidase; PGE electrode