title: Establishment and multi drug resistance evolution of ST235 Pseudomonas aeruginosa strains in the intensive care unit of a Colombian hospital.
abstract: Drug resistant Pseudomonas aeruginosa represents a therapeutic challenge. To assess the diversity of P. aeruginosa antibiotic resistant variants, isolates were recovered from hospital patients in Colombia. Thirty of 60 isolates contained class 1 integrons and five were of Sequence Type ST235 having appeared in a single intensive care unit. All five possessed an unusual integron but showed differences in gene cassette content and the presence/absence of insertion sequence IS26. This showed that differences can arise rapidly, even within a single ICU. Also, the emergence of IS26 in P. aeruginosa is contributing to the evolution of resistance in this bacterium.
authors.affiliation: The ithree Institute, University of Technology, P.O. Box 123, Broadway, Sydney, NSW 2007, Australia. Electronic address: e.martinez@centenary.org.au.
authors.forename: Elena
authors.initials: E
authors.lastname: Martinez
authors.affiliation: Laboratorio de Genética Molecular Bacteriana, Universidad El Bosque, Bogotá, Colombia. Electronic address: javiesco21@yahoo.com.
authors.forename: Javier Escobar
authors.initials: JE
authors.lastname: Pérez
authors.affiliation: Grupo de Resistencia Bacteriana y Enfermedades Tropicales, Universidad del Sinú, Monteria-Cordoba, Colombia. Electronic address: buelvasfrancisco@hotmail.com.
authors.forename: Francisco
authors.initials: F
authors.lastname: Buelvas
authors.affiliation: Grupo de Resistencia Bacteriana y Enfermedades Tropicales, Universidad del Sinú, Monteria-Cordoba, Colombia. Electronic address: ncatalinatv@gmail.com.
authors.forename: Catalina
authors.initials: C
authors.lastname: Tovar
authors.affiliation: The ithree Institute, University of Technology, P.O. Box 123, Broadway, Sydney, NSW 2007, Australia; Laboratorio de Genética Molecular Bacteriana, Universidad El Bosque, Bogotá, Colombia. Electronic address: natashavanegas@yahoo.es.
authors.forename: Natasha
authors.initials: N
authors.lastname: Vanegas
authors.affiliation: The ithree Institute, University of Technology, P.O. Box 123, Broadway, Sydney, NSW 2007, Australia. Electronic address: algernonspearpoint@gmail.com.
authors.forename: H W
authors.initials: HW
authors.lastname: Stokes
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D003105:Colombia / Q000453:epidemiology; D003428:Cross Infection / Q000453:epidemiology / Q000382:microbiology*; D024901:Drug Resistance, Multiple, Bacterial; D006801:Humans; D007362:Intensive Care Units / Q000706:statistics & numerical data; D008826:Microbial Sensitivity Tests; D011552:Pseudomonas Infections / Q000453:epidemiology / Q000382:microbiology*; D011550:Pseudomonas aeruginosa / Q000145:classification / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D000900:Anti-Bacterial Agents
keywords: Antibiotic resistance; Class 1 integron; Hospital acquired infections; IS26; Pseudomonas aeruginosa ST235