title: [Effects. of N-acetylcysteine on fluoride-induced endoplasmic reticulum stress in sertoli cells].
abstract: OBJECTIVE
  Investigated the effects of N-acetylcysteine (NAC) on endoplasmic reticulum stress of sertoli cells induced by sodium fluoride (NaF).
  
  
  METHODS
  Rat sertoli cells were exposed to various concentration of (0, 6, 12, 24 µg/ml) sodium fluoride with or without 2 mmol/L NAC for 24 hours. The cell viability was evaluated using trypan blue exclusion test. Intracellular reactive oxygen species (ROS) was measured using the fluorescent probe DCFH-DA. Western blot was used to test the expression of GRP78, PERK and CHOP.
  
  
  RESULTS
  It was found that treatment with NAC (2 mmol/L) restored the reduced cell viability and excessive oxidative stress (P < 0.01). Moreover, fluoride exposure upregulated the expression of GRP7 8, PERK and CHOP protein (P <0. 01 ). NAC was also found to suppress the levels of GRP78, PERK and CHOP expression in NaF-treated cells (p<0.01).
  
  
  CONCLUSIONS
  Endoplasmic reticulum stress signaling pathways were activated by ROS, and NAC attenuate endoplasmic reticulum stress through inhibiting the levels of ROS in NaF-treated sertoli cells.
authors.forename: Yang
authors.initials: Y
authors.lastname: Yang
authors.forename: Hui
authors.initials: H
authors.lastname: Huang
authors.forename: Demin
authors.initials: D
authors.lastname: Feng
authors.forename: Wenyi
authors.initials: W
authors.lastname: Liu
authors.forename: Xuemin
authors.initials: X
authors.lastname: Cheng
authors.forename: Yue
authors.initials: Y
authors.lastname: Ba
authors.forename: Liuxin
authors.initials: L
authors.lastname: Cui
mesh_terms: D000111:Acetylcysteine / Q000494:pharmacology*; D000818:Animals; D002470:Cell Survival / Q000187:drug effects*; D000091342:Endoplasmic Reticulum Chaperone BiP; D059865:Endoplasmic Reticulum Stress / Q000187:drug effects*; D005452:Fluoresceins; D006360:Heat-Shock Proteins; D006801:Humans; D008297:Male; D018384:Oxidative Stress; D051381:Rats; D017382:Reactive Oxygen Species / Q000009:adverse effects; D012708:Sertoli Cells / Q000187:drug effects; D015398:Signal Transduction; D012969:Sodium Fluoride
chemical_list: D000091342:Endoplasmic Reticulum Chaperone BiP; D005452:Fluoresceins; C000717247:HSPA5 protein, human; D006360:Heat-Shock Proteins; D017382:Reactive Oxygen Species; C029569:diacetyldichlorofluorescein; D012969:Sodium Fluoride; D000111:Acetylcysteine