title: Chemical and biochemical approaches to the production of 7-hydroxylated C19-steroids.
abstract: Abstract Interest has developed into 7-hydroxylated derivatives of 3β-hydroxylated C19-steroids, such as dehydroepiandrosterone (DHEA) and epiandrosterone because of their effects on inflammation, immune response, and cell repair. These steroids are not currently available from commercial sources, and it is necessary to produce them for relevant studies. We report here the chemical and biochemical approaches that were used for their production. Simplified chemical approaches lead to the production of 7α-/7β-hydroxy-DHEA and 7β-hydroxy-epiandrosterone in gram quantities, which are made available to researchers. Biochemical approaches were used to produce isotope-labeled compounds. Thus, 2H-, 3H-, and 14C-labeled 7α-/7β-hydroxy-DHEA and 7β-hydroxy-epiandrosterone could be produced in quantities sufficient for use in investigations into their mode of action.
authors.forename: Clotilde
authors.initials: C
authors.lastname: Ferroud
authors.forename: Gilbert
authors.initials: G
authors.lastname: Revial
authors.forename: Robert
authors.initials: R
authors.lastname: Morfin