title: Modulation in the rheological behaviour of porcine pepsin treated guar galactomannan on admixture with κ-carrageenan.
abstract: The non-specific action of pepsin on guar galactomannan (GG) resulted in selective removal of galactose residues, leading to the formation of galactose - depleted guar galactomannan (GDGG) with decrease in Mw and change in G: M ratio of 1:3.4, thus mimicking that of locust bean gum (LBG). Admixture of GDGG with κ-carrageenan revealed a two fold increase in the magnitude of elasticity (G') compared to κ-carrageenan alone, suggesting a synergistic interaction similar to LBG with κ-carrageenan. Blends of GDGG and LBG with κ-carrageenan at a concentration of 40% (w/w, 0.8% total biopolymer) showed a maximum increase in G'. The GDGG/κ-carrageenan blend also showed a Tm of 47°C, similar to LBG/κ-carrageenan blend. Thus, debranching of guar galactomannan by the catalytic action of pepsin is beneficial for improved functional properties and diversified applications.
authors.affiliation: Department of Biochemistry and Nutrition, Central Food Technological Research Institute, Council of Scientific and Industrial Research, Mysore - 570 020, India. Electronic address: drshobhams14@gmail.com.
authors.forename: Shobha S
authors.initials: SS
authors.lastname: Mysore
authors.affiliation: Department of Biochemistry and Nutrition, Central Food Technological Research Institute, Council of Scientific and Industrial Research, Mysore - 570 020, India. Electronic address: tharanathan@yahoo.co.uk.
authors.forename: Tharanathan N
authors.initials: TN
authors.lastname: Rudrapatnam
mesh_terms: D000818:Animals; D002351:Carrageenan / Q000737:chemistry*; D031293:Cyamopsis / Q000737:chemistry*; D005685:Galactans / Q000737:chemistry; D005690:Galactose / Q000031:analogs & derivatives; D008351:Mannans / Q000737:chemistry*; D010434:Pepsin A / Q000378:metabolism*; D053149:Plant Gums / Q000737:chemistry; D012212:Rheology; D013329:Structure-Activity Relationship; D013552:Swine; D013696:Temperature
chemical_list: D005685:Galactans; D008351:Mannans; D053149:Plant Gums; C012990:galactomannan; D002351:Carrageenan; D010434:Pepsin A; C017471:locust bean gum; D005690:Galactose
keywords: Guar galactomannan; Locust bean gum; Pepsin; Synergy; κ-Carrageenan