title: Integrative approach to produce hydrogen and polyhydroxybutyrate from biowaste using defined bacterial cultures.
abstract: Biological production of hydrogen (H2) and polyhydroxybutyrate (PHB) from pea-shell slurry (PSS) was investigated using defined mixed culture (MMC4, composed of Enterobacter, Proteus, Bacillus spp.). Under batch culture, 19.0LH2/kg of PSS (total solid, TS, 2%w/v) was evolved. Using effluent from the H2 producing stage, Bacillus cereus EGU43 could produce 12.4% (w/w) PHB. Dilutions of PSS hydrolysate containing glucose (0.5%, w/v) resulted in 45-75LH2/kg TS fed and 19.1% (w/w) of PHB content. Under continuous culture, MMC4 immobilized on coconut coir (CC) lead to an H2 yield of 54L/kg TS fed and a PHB content of 64.7% (w/w). An improvement of 2- and 3.7-fold in H2 and PHB yields were achieved in comparison to control. This integrative approach using defined set of bacterial strains can prove effective in producing biomolecules from biowastes.
authors.affiliation: Microbial Biotechnology and Genomics, CSIR - Institute of Genomics and Integrative Biology (IGIB), Delhi University Campus, Mall Road, Delhi 110007, India; Department of Chemical Engineering, Konkuk University, 1 Hwayang-Dong, Gwangjin-Gu, Seoul 143-701, Republic of Korea.
authors.forename: Sanjay K S
authors.initials: SK
authors.lastname: Patel
authors.affiliation: Microbial Biotechnology and Genomics, CSIR - Institute of Genomics and Integrative Biology (IGIB), Delhi University Campus, Mall Road, Delhi 110007, India; Department of Biotechnology, Pune University, Pune 411007, India.
authors.forename: Prasun
authors.initials: P
authors.lastname: Kumar
authors.affiliation: Microbial Biotechnology and Genomics, CSIR - Institute of Genomics and Integrative Biology (IGIB), Delhi University Campus, Mall Road, Delhi 110007, India.
authors.forename: Mamtesh
authors.initials: M
authors.lastname: Singh
authors.affiliation: Department of Chemical Engineering, Konkuk University, 1 Hwayang-Dong, Gwangjin-Gu, Seoul 143-701, Republic of Korea.
authors.forename: Jung-Kul
authors.initials: JK
authors.lastname: Lee
authors.affiliation: Microbial Biotechnology and Genomics, CSIR - Institute of Genomics and Integrative Biology (IGIB), Delhi University Campus, Mall Road, Delhi 110007, India. Electronic address: vckalia@igib.res.in.
authors.forename: Vipin C
authors.initials: VC
authors.lastname: Kalia
mesh_terms: D001419:Bacteria / Q000378:metabolism* / Q000648:ultrastructure; D019149:Bioreactors; D001709:Biotechnology / Q000379:methods*; D018929:Cell Culture Techniques; D002849:Chromatography, Gas; D006859:Hydrogen / Q000378:metabolism*; D006868:Hydrolysis; D006885:Hydroxybutyrates / Q000378:metabolism*; D046529:Microscopy, Electron, Transmission; D018532:Pisum sativum / Q000737:chemistry; D011108:Polymers / Q000378:metabolism*; D017550:Spectroscopy, Fourier Transform Infrared; D014865:Waste Disposal, Fluid / Q000379:methods*
chemical_list: D006885:Hydroxybutyrates; D011108:Polymers; D006859:Hydrogen
keywords: Bacillus; Dark fermentation; Hydrogen; Mixed bacterial culture; Polyhydroxyalkanoates