title: Polysaccharides produced by Enterobacter cloacae induce apoptosis in cervical cancer cells.
abstract: The effects and possible mechanism of polysaccharides produced by Enterobacter cloacae (EPS) on cervical cancer cells (Hela and SiHa) were investigated in vitro in the current study. The results showed that supplementation of EPS inhibited the proliferation of HeLa and SiHa cells in a concentration- and time-dependent manner. In addition, the EPS also induced the apoptosis of the cervical cancer cells by changing the nuclear morphology and decreasing the mitochondrial membrane potential. Furthermore, the EPS-induced apoptosis was associated with the regulation of Bcl-2 family protein expression. In particular, the pro-apoptotic protein Bak-1 was increased and anti-apoptotic protein Bcl-2 was decreased with the increasing concentration at certain range of EPS. These results indicated that EPS might induce the apoptosis of cervical cancer cells through an intrinsic apoptotic pathway, and EPS may act as potent antitumor agents.
authors.affiliation: Department of Reproductive Medicine, The First Affiliated Hospital of Medical College, Xi'an Jiaotong University, Xi'an, China; Division of Cancer Stem Cell Research, Key Laboratory of Environment and Genes Related to Diseases, Ministry of Education of the People's Republic of China, Xi'an, China.
authors.forename: Ke
authors.initials: K
authors.lastname: Zhao
authors.affiliation: School of Life Sciences, Northwestern Polytechnical University, 127 Youyi Xilu, Xi'an 710072, China.
authors.forename: Mingliang
authors.initials: M
authors.lastname: Jin
authors.affiliation: Department of Reproductive Medicine, The First Affiliated Hospital of Medical College, Xi'an Jiaotong University, Xi'an, China; Division of Cancer Stem Cell Research, Key Laboratory of Environment and Genes Related to Diseases, Ministry of Education of the People's Republic of China, Xi'an, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Chen
authors.affiliation: Department of Reproductive Medicine, The First Affiliated Hospital of Medical College, Xi'an Jiaotong University, Xi'an, China; Division of Cancer Stem Cell Research, Key Laboratory of Environment and Genes Related to Diseases, Ministry of Education of the People's Republic of China, Xi'an, China. Electronic address: zpsheng@mail.xjtu.edu.cn.
authors.forename: Peng-Sheng
authors.initials: PS
authors.lastname: Zheng
mesh_terms: D000970:Antineoplastic Agents / Q000008:administration & dosage* / Q000737:chemistry; D017209:Apoptosis / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D016972:Enterobacter cloacae / Q000737:chemistry; D005260:Female; D006367:HeLa Cells; D006801:Humans; D011134:Polysaccharides / Q000008:administration & dosage* / Q000737:chemistry; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000096:biosynthesis; D015398:Signal Transduction / Q000187:drug effects; D002583:Uterine Cervical Neoplasms / Q000188:drug therapy* / Q000473:pathology
chemical_list: D000970:Antineoplastic Agents; D011134:Polysaccharides; D019253:Proto-Oncogene Proteins c-bcl-2
keywords: Antitumor activities; Enterobacter cloacae; Polysaccharides