title: Stress responses to trichlorophenol in Arabidopsis and integrative analysis of alteration in transcriptional profiling from microarray.
abstract: Trichlorophenols, also known as TCPs, are one of the most persistent environmental pollutants. It is a matter of concern as they are toxic and cumulative in soil and water bodies which could lead to serious consequences to the biosphere. How plants respond to this compound has rarely been examined previously. In our study, detailed morphological and physiological responses of Arabidopsis to 2,4,6-trichlorophenol, a representative TCP, were investigated. Seed germination and seedling growth were markedly inhibited by 2,4,6-TCP. Furthermore, we performed gene expression profiling analysis upon 2,4,6-TCP treatment in Arabidopsis and identified 34 transcripts induced and 212 repressed more than four folds. Gene Ontology (GO) analysis showed that these TCP-responsive genes are involved in various biological processes, such as secondary metabolism, biological regulation, response to stimulus and other processes related to growth and development. The activities of two reactive oxygen species-related enzymes (peroxidase and superoxide dismutase) and the content of malondialdehyde were increased significantly after 2,4,6-TCP treatment. Our findings have the potential to provide valuable gene resources and theoretical information for more in-depth analyses of TCPs' response in Arabidopsis thaliana and even other organic pollutants.
authors.affiliation: Shanghai Key Laboratory of Agricultural Genetics and Breeding, Agro-Biotechnology Research Institute, Shanghai Academy of Agricultural Sciences, 2901 Beidi Rd, Shanghai 201106, PR China.
authors.forename: Zhenjun
authors.initials: Z
authors.lastname: Li
authors.affiliation: Shanghai Key Laboratory of Agricultural Genetics and Breeding, Agro-Biotechnology Research Institute, Shanghai Academy of Agricultural Sciences, 2901 Beidi Rd, Shanghai 201106, PR China.
authors.forename: Bo
authors.initials: B
authors.lastname: Zhu
authors.affiliation: Shanghai Key Laboratory of Agricultural Genetics and Breeding, Agro-Biotechnology Research Institute, Shanghai Academy of Agricultural Sciences, 2901 Beidi Rd, Shanghai 201106, PR China.
authors.forename: Bo
authors.initials: B
authors.lastname: Wang
authors.affiliation: Shanghai Key Laboratory of Agricultural Genetics and Breeding, Agro-Biotechnology Research Institute, Shanghai Academy of Agricultural Sciences, 2901 Beidi Rd, Shanghai 201106, PR China.
authors.forename: Jianjie
authors.initials: J
authors.lastname: Gao
authors.affiliation: Shanghai Key Laboratory of Agricultural Genetics and Breeding, Agro-Biotechnology Research Institute, Shanghai Academy of Agricultural Sciences, 2901 Beidi Rd, Shanghai 201106, PR China.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Fu
authors.affiliation: Shanghai Key Laboratory of Agricultural Genetics and Breeding, Agro-Biotechnology Research Institute, Shanghai Academy of Agricultural Sciences, 2901 Beidi Rd, Shanghai 201106, PR China. Electronic address: qhyao_saas@163.com.
authors.forename: Quanhong
authors.initials: Q
authors.lastname: Yao
mesh_terms: D017360:Arabidopsis / Q000187:drug effects* / Q000235:genetics*; D015415:Biomarkers / Q000378:metabolism; D002733:Chlorophenols / Q000737:chemistry*; D004305:Dose-Response Relationship, Drug; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D018525:Germination / Q000187:drug effects; D020411:Oligonucleotide Array Sequence Analysis; D009930:Organic Chemicals / Q000737:chemistry; D009195:Peroxidase / Q000378:metabolism; D010641:Phenotype; D017382:Reactive Oxygen Species / Q000378:metabolism; D036226:Seedlings / Q000187:drug effects / Q000378:metabolism; D012639:Seeds / Q000187:drug effects / Q000378:metabolism; D012989:Soil Pollutants / Q000737:chemistry; D013312:Stress, Physiological; D013482:Superoxide Dismutase / Q000378:metabolism; D014158:Transcription, Genetic
chemical_list: D015415:Biomarkers; D002733:Chlorophenols; D009930:Organic Chemicals; D017382:Reactive Oxygen Species; D012989:Soil Pollutants; D009195:Peroxidase; D013482:Superoxide Dismutase; C024564:2,4,6-trichlorophenol
keywords: Arabidopsis; Gene Ontology; Gene expression; Reactive oxygen species; Trichlorophenol