title: The development of diet-induced obesity and associated metabolic impairments in Dj-1 deficient mice.
abstract: DJ-1 constitutes a ubiquitously expressed, oxidative stress-responsive protein with multiple functions. DJ-1 emerged as a candidate from our previous proteome analysis investigating alterations in the hypothalamus in three mouse strains differing in their susceptibility to diet-induced obesity (DIO). Validation studies demonstrated a high-fat diet (HFD)-induced shift in the DJ-1 isoform pattern in the hypothalamus and several other tissues of mice. Others found HFD-induced alterations in DJ-1 protein abundance in adipose tissue and pancreatic islets in wild-type rodents. Here, we investigated the gene-diet interaction by challenging Dj-1(-/-) mice with a HFD. We demonstrate that the development of diet-induced obesity (DIO) Dj-1(-/-) mice is according to wild-type mice with the exception of transient higher gains in fat mass at the expense of lean mass after 14 weeks of feeding.
authors.affiliation: Molecular Nutritional Medicine, Technische Universität München, Else Kröner-Fresenius-Zentrum (EKFZ) & Research Center for Nutrition and Food Sciences (ZIEL), Gregor-Mendel-Str. 2, 85350 Freising-Weihenstephan, Germany.
authors.forename: Katrin
authors.initials: K
authors.lastname: Seyfarth
authors.affiliation: Heinrich-Heine-Universität Düsseldorf, Molecular Proteomics Laboratory, BMFZ, Universitätsstr. 1, 40225 Düsseldorf, Germany.
authors.forename: Gereon
authors.initials: G
authors.lastname: Poschmann
authors.affiliation: Molecular Nutritional Medicine, Technische Universität München, Else Kröner-Fresenius-Zentrum (EKFZ) & Research Center for Nutrition and Food Sciences (ZIEL), Gregor-Mendel-Str. 2, 85350 Freising-Weihenstephan, Germany.
authors.forename: Jan
authors.initials: J
authors.lastname: Rozman
authors.affiliation: Molecular Nutritional Medicine, Technische Universität München, Else Kröner-Fresenius-Zentrum (EKFZ) & Research Center for Nutrition and Food Sciences (ZIEL), Gregor-Mendel-Str. 2, 85350 Freising-Weihenstephan, Germany.
authors.forename: Tobias
authors.initials: T
authors.lastname: Fromme
authors.affiliation: Molecular Nutritional Medicine, Technische Universität München, Else Kröner-Fresenius-Zentrum (EKFZ) & Research Center for Nutrition and Food Sciences (ZIEL), Gregor-Mendel-Str. 2, 85350 Freising-Weihenstephan, Germany.
authors.forename: Nadine
authors.initials: N
authors.lastname: Rink
authors.affiliation: Helmholtz Zentrum München, Institute of Developmental Genetics, Munich, Germany; Max Planck Institute of Psychiatr, Kraepelinstr. 2-10, 80804 Munich, Germany; Technische Universität München-Weihenstephan, Chair of Developmental Genetics c/o Helmholtz Zentrum München, Ingolstädter Landstr. 1,85764 Neuherberg/Munich, Germany. German Center for Neurodegenerative Diseases (DZNE) Site Munich, Schillerstr. 44,80336 Munich, Germany; Munich Cluster for Systems Neurology (SyNergy), Adolf-Butenandt-Institut Ludwig-Maximilians-Universität München, Schillerstr. 44, 80336 Munich, Germany.
authors.forename: Andreas
authors.initials: A
authors.lastname: Hofmann
authors.affiliation: Helmholtz Zentrum München, Institute of Developmental Genetics, Munich, Germany; Max Planck Institute of Psychiatr, Kraepelinstr. 2-10, 80804 Munich, Germany; Technische Universität München-Weihenstephan, Chair of Developmental Genetics c/o Helmholtz Zentrum München, Ingolstädter Landstr. 1,85764 Neuherberg/Munich, Germany. German Center for Neurodegenerative Diseases (DZNE) Site Munich, Schillerstr. 44,80336 Munich, Germany; Munich Cluster for Systems Neurology (SyNergy), Adolf-Butenandt-Institut Ludwig-Maximilians-Universität München, Schillerstr. 44, 80336 Munich, Germany.
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Wurst
authors.affiliation: Heinrich-Heine-Universität Düsseldorf, Molecular Proteomics Laboratory, BMFZ, Universitätsstr. 1, 40225 Düsseldorf, Germany.
authors.forename: Kai
authors.initials: K
authors.lastname: Stühler
authors.affiliation: Molecular Nutritional Medicine, Technische Universität München, Else Kröner-Fresenius-Zentrum (EKFZ) & Research Center for Nutrition and Food Sciences (ZIEL), Gregor-Mendel-Str. 2, 85350 Freising-Weihenstephan, Germany. Electronic address: mk@tum.de.
authors.forename: Martin
authors.initials: M
authors.lastname: Klingenspor
mesh_terms: D000273:Adipose Tissue / Q000378:metabolism; D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D002153:Calorimetry, Indirect; D059305:Diet, High-Fat / Q000009:adverse effects*; D002149:Energy Intake; D005260:Female; D005951:Glucose Tolerance Test; D007031:Hypothalamus / Q000378:metabolism; D007328:Insulin / Q000097:blood; D007515:Islets of Langerhans / Q000378:metabolism; D020738:Leptin / Q000008:administration & dosage / Q000097:blood; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D009765:Obesity / Q000503:physiopathology*; D015513:Oncogene Proteins / Q000172:deficiency* / Q000235:genetics; D018384:Oxidative Stress; D054464:Peroxiredoxins / Q000172:deficiency* / Q000235:genetics; D000071617:Protein Deglycase DJ-1; D060888:Real-Time Polymerase Chain Reaction; D050796:STAT3 Transcription Factor / Q000235:genetics / Q000378:metabolism; D017423:Sequence Analysis, RNA
chemical_list: D001786:Blood Glucose; D007328:Insulin; D020738:Leptin; D015513:Oncogene Proteins; D050796:STAT3 Transcription Factor; C494087:Stat3 protein, mouse; D054464:Peroxiredoxins; C503466:PARK7 protein, mouse; D000071617:Protein Deglycase DJ-1
keywords: DJ-1; Diet-induced obesity; High-fat diet; Mice; Neurodegeneration; Park7