title: Terpenoids with alpha-glucosidase inhibitory activity from the submerged culture of Inonotus obliquus.
abstract: Lanostane-type triterpenoids, inotolactones A and B, a drimane-type sesquiterpenoid, inotolactone C, and five known terpenoids 6β-hydroxy-trans-dihydroconfertifolin, inotodiol, 3β,22-dihydroxyanosta-7,9(11),24-triene, 3β-hydroxycinnamolide, and 17-hydroxy-ent-atisan-19-oic acid, were isolated from the submerged culture of chaga mushroom, Inonotus obliquus. Their structures were characterized by spectroscopic methods, including MS and NMR (1D and 2D) spectroscopic techniques. Inotolactones A and B, examples of lanostane-type triterpenoids bearing α,β-dimethyl, α,β-unsaturated δ-lactone side chains, exhibited more potent alpha-glucosidase inhibitory activities than the positive control acarbose. This finding might be related to the anti-hyperglycemic properties of the fungus and to its popular role as a diabetes treatment. In addition, a drimane-type sesquiterpenoid and an atisane-type diterpenoid were isolated from I. obliquus.
authors.affiliation: College of Pharmaceutical Science, Zhejiang University of Technology, 18 Chaowang Road, Hangzhou 310014, PR China.
authors.forename: You-Min
authors.initials: YM
authors.lastname: Ying
authors.affiliation: College of Pharmaceutical Science, Zhejiang University of Technology, 18 Chaowang Road, Hangzhou 310014, PR China.
authors.forename: Lin-Yan
authors.initials: LY
authors.lastname: Zhang
authors.affiliation: Institute of Biotechnology, Hangzhou East China Pharmaceutical Group, 866 Moganshan Road, Hangzhou 310011, PR China.
authors.forename: Xia
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Institute of Biotechnology, Hangzhou East China Pharmaceutical Group, 866 Moganshan Road, Hangzhou 310011, PR China.
authors.forename: Hai-Bo
authors.initials: HB
authors.lastname: Bai
authors.affiliation: College of Pharmaceutical Science, Zhejiang University of Technology, 18 Chaowang Road, Hangzhou 310014, PR China.
authors.forename: Dong-E
authors.initials: DE
authors.lastname: Liang
authors.affiliation: College of Pharmaceutical Science, Zhejiang University of Technology, 18 Chaowang Road, Hangzhou 310014, PR China.
authors.forename: Lie-Feng
authors.initials: LF
authors.lastname: Ma
authors.affiliation: College of Pharmaceutical Science, Zhejiang University of Technology, 18 Chaowang Road, Hangzhou 310014, PR China. Electronic address: tianranyaowu@zjut.edu.cn.
authors.forename: Wei-Guang
authors.initials: WG
authors.lastname: Shan
authors.affiliation: College of Pharmaceutical Science, Zhejiang University of Technology, 18 Chaowang Road, Hangzhou 310014, PR China. Electronic address: zjnpr@zjut.edu.cn.
authors.forename: Zha-Jun
authors.initials: ZJ
authors.lastname: Zhan
mesh_terms: D000363:Agaricales / Q000737:chemistry*; D065089:Glycoside Hydrolase Inhibitors / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D006575:Heterocyclic Compounds, 3-Ring / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D007810:Lanosterol / Q000031:analogs & derivatives / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D000520:alpha-Glucosidases / Q000187:drug effects
chemical_list: C445942:3-hydroxycinnamolide; D065089:Glycoside Hydrolase Inhibitors; D006575:Heterocyclic Compounds, 3-Ring; C517091:inotodiol; D007810:Lanosterol; D000520:alpha-Glucosidases
keywords: Alpha-glucosidase; Chaga mushroom; Drimane; Hymenochaetaceae; Inonotus obliquus; Lanostane; Triterpenoids