title: Response of aerobic granular sludge to the long-term presence to nanosilver in sequencing batch reactors: reactor performance, sludge property, microbial activity and community.
abstract: The increasing use of silver nanoparticles (Ag NPs) raises concerns about their potential toxic effects on the environment. Granular shape sludge is a special type of microbial aggregate. The response of aerobic granular sludge (AGS) to the long-term presence of Ag NPs has not been well studied. In this study, AGS was exposed to 5 and 50mg/L Ag NPs in sequence batch reactors (SBRs) for 69 days, and its response was evaluated based on the sludge properties, microbial activity and community, and reactor performance. The results showed that Ag NPs caused inhibition to microbial activities of AGS from Day 35. At the end of 69 days of Ag NPs exposure, the microbial activity of AGS was significantly inhibited in terms of inhibitions of the ammonia oxidizing rate (33.0%), respiration rate (17.7% and 45.6%) and denitrification rate (6.8%), as well as decreases in the ammonia mono-oxygenase and nitrate reductase activities. During the long-term exposure, the AGS maintained its granular shape and large granule size (approximately 900 μm); the microbial community of AGS slightly changed, but the dominant microbial population remained. Overall, the AGS tolerated the toxicity of Ag NPs well, but a long-term exposure may produce chronic toxicity to the AGS, which is concerning.
authors.affiliation: Key Laboratory of Water and Sediment Sciences of Ministry of Education/State Key Laboratory of Water Environment Simulation, School of Environment, Beijing Normal University, Beijing 100875, PR China. Electronic address: xchquan@bnu.edu.cn.
authors.forename: Xiangchun
authors.initials: X
authors.lastname: Quan
authors.affiliation: Key Laboratory of Water and Sediment Sciences of Ministry of Education/State Key Laboratory of Water Environment Simulation, School of Environment, Beijing Normal University, Beijing 100875, PR China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Cen
authors.affiliation: Key Laboratory of Water and Sediment Sciences of Ministry of Education/State Key Laboratory of Water Environment Simulation, School of Environment, Beijing Normal University, Beijing 100875, PR China.
authors.forename: Fang
authors.initials: F
authors.lastname: Lu
authors.affiliation: Key Laboratory of Water and Sediment Sciences of Ministry of Education/State Key Laboratory of Water Environment Simulation, School of Environment, Beijing Normal University, Beijing 100875, PR China.
authors.forename: Lingyun
authors.initials: L
authors.lastname: Gu
authors.affiliation: Key Laboratory of Water and Sediment Sciences of Ministry of Education/State Key Laboratory of Water Environment Simulation, School of Environment, Beijing Normal University, Beijing 100875, PR China.
authors.forename: Jingyun
authors.initials: J
authors.lastname: Ma
mesh_terms: D000332:Aerobiosis; D019149:Bioreactors / Q000382:microbiology*; D053768:Metal Nanoparticles / Q000633:toxicity*; D010088:Oxidoreductases / Q000378:metabolism; D012722:Sewage / Q000737:chemistry / Q000382:microbiology*; D012834:Silver / Q000633:toxicity*; D014865:Waste Disposal, Fluid / Q000379:methods*
chemical_list: D012722:Sewage; D012834:Silver; D010088:Oxidoreductases; C045582:ammonia monooxygenase
keywords: Aerobic granular sludge; Inhibition; Microbial community; Silver nanoparticle