title: Primate lentiviruses are differentially inhibited by interferon-induced transmembrane proteins.
abstract: Interferon-induced transmembrane (IFITM) proteins inhibit the entry of a large number of viruses. Not surprisingly, many viruses are refractory to this inhibition. In this study, we report that different strains of HIV and SIV are inhibited by human IFITM proteins to various degrees, with SIV of African green monkeys (SIV(AGM)) being mostly restricted by human IFITM2. Interestingly, SIV(AGM) is as much inhibited by human IFITM2 as by IFITM3 of its own host African green monkeys. Our data further demonstrate that the entry of SIV(AGM) is impaired by human IFITM2 and that this inhibition is overcome by the cholesterol-binding compound amphotericin B that also overcomes IFITM inhibition of influenza A viruses. These results suggest that IFITM proteins exploit similar mechanisms to inhibit the entry of both pH-independent primate lentiviruses and the pH-dependent influenza A viruses.
authors.affiliation: Lady Davis Institute, Jewish General Hospital, Montreal, Quebec, Canada H3T 1E2; Department of Medicine, McGill University, Montreal, Quebec, Canada H3A 2B4.
authors.forename: Jin
authors.initials: J
authors.lastname: Qian
authors.affiliation: Lady Davis Institute, Jewish General Hospital, Montreal, Quebec, Canada H3T 1E2.
authors.forename: Yann
authors.initials: Y
authors.lastname: Le Duff
authors.affiliation: Lady Davis Institute, Jewish General Hospital, Montreal, Quebec, Canada H3T 1E2; Department of Medicine, McGill University, Montreal, Quebec, Canada H3A 2B4.
authors.forename: Yimeng
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Lady Davis Institute, Jewish General Hospital, Montreal, Quebec, Canada H3T 1E2.
authors.forename: Qinghua
authors.initials: Q
authors.lastname: Pan
authors.affiliation: Lady Davis Institute, Jewish General Hospital, Montreal, Quebec, Canada H3T 1E2; Department of Microbiology and Immunology, McGill University, Montreal, Quebec, Canada H3A 2B4.
authors.forename: Shilei
authors.initials: S
authors.lastname: Ding
authors.affiliation: Department of Molecular Microbiology & Immunology, School of Medicine, Bond Life Sciences Center, University of Missouri, Columbia, MO 65211-7310, USA.
authors.forename: Yi-Min
authors.initials: YM
authors.lastname: Zheng
authors.affiliation: Department of Molecular Microbiology & Immunology, School of Medicine, Bond Life Sciences Center, University of Missouri, Columbia, MO 65211-7310, USA.
authors.forename: Shan-Lu
authors.initials: SL
authors.lastname: Liu
authors.affiliation: Lady Davis Institute, Jewish General Hospital, Montreal, Quebec, Canada H3T 1E2; Department of Medicine, McGill University, Montreal, Quebec, Canada H3A 2B4; Department of Microbiology and Immunology, McGill University, Montreal, Quebec, Canada H3A 2B4. Electronic address: chen.liang@mcgill.ca.
authors.forename: Chen
authors.initials: C
authors.lastname: Liang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D019556:COS Cells; D002522:Chlorocebus aethiops; D057809:HEK293 Cells; D006678:HIV / Q000276:immunology / Q000472:pathogenicity / Q000502:physiology; D054884:Host-Pathogen Interactions / Q000276:immunology*; D006801:Humans; D009980:Influenza A virus / Q000276:immunology / Q000472:pathogenicity / Q000502:physiology; D007372:Interferons / Q000276:immunology*; D017958:Lentiviruses, Primate / Q000276:immunology / Q000472:pathogenicity* / Q000502:physiology; D008565:Membrane Proteins / Q000235:genetics / Q000276:immunology*; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid; D015302:Simian Immunodeficiency Virus / Q000276:immunology / Q000472:pathogenicity / Q000502:physiology; D014709:Vero Cells; D053586:Virus Internalization
chemical_list: D008565:Membrane Proteins; D007372:Interferons
keywords: HIV; IFITM; SIV; Virus entry