title: The parathyroid hormone-regulated transcriptome in osteocytes: parallel actions with 1,25-dihydroxyvitamin D3 to oppose gene expression changes during differentiation and to promote mature cell function.
abstract: Although localized to the mineralized matrix of bone, osteocytes are able to respond to systemic factors such as the calciotropic hormones 1,25(OH)2D3 and PTH. In the present studies, we examined the transcriptomic response to PTH in an osteocyte cell model and found that this hormone regulated an extensive panel of genes. Surprisingly, PTH uniquely modulated two cohorts of genes, one that was expressed and associated with the osteoblast to osteocyte transition and the other a cohort that was expressed only in the mature osteocyte. Interestingly, PTH's effects were largely to oppose the expression of differentiation-related genes in the former cohort, while potentiating the expression of osteocyte-specific genes in the latter cohort. A comparison of the transcriptional effects of PTH with those obtained previously with 1,25(OH)2D3 revealed a subset of genes that was strongly overlapping. While 1,25(OH)2D3 potentiated the expression of osteocyte-specific genes similar to that seen with PTH, the overlap between the two hormones was more limited. Additional experiments identified the PKA-activated phospho-CREB (pCREB) cistrome, revealing that while many of the differentiation-related PTH regulated genes were apparent targets of a PKA-mediated signaling pathway, a reduction in pCREB binding at sites associated with osteocyte-specific PTH targets appeared to involve alternative PTH activation pathways. That pCREB binding activities positioned near important hormone-regulated gene cohorts were localized to control regions of genes was reinforced by the presence of epigenetic enhancer signatures exemplified by unique modifications at histones H3 and H4. These studies suggest that both PTH and 1,25(OH)2D3 may play important and perhaps cooperative roles in limiting osteocyte differentiation from its precursors while simultaneously exerting distinct roles in regulating mature osteocyte function. Our results provide new insight into transcription factor-associated mechanisms through which PTH and 1,25(OH)2D3 regulate a plethora of genes important to the osteoblast/osteocyte lineage.
authors.affiliation: Department of Biochemistry, University of Wisconsin-Madison, Madison, WI 53706, USA.
authors.forename: Hillary C
authors.initials: HC
authors.lastname: St John
authors.affiliation: Department of Biochemistry, University of Wisconsin-Madison, Madison, WI 53706, USA.
authors.forename: Mark B
authors.initials: MB
authors.lastname: Meyer
authors.affiliation: Department of Biochemistry, University of Wisconsin-Madison, Madison, WI 53706, USA.
authors.forename: Nancy A
authors.initials: NA
authors.lastname: Benkusky
authors.affiliation: Department of Biochemistry, University of Wisconsin-Madison, Madison, WI 53706, USA.
authors.forename: Alex H
authors.initials: AH
authors.lastname: Carlson
authors.affiliation: Department of Oral Biology, School of Dentistry, University of Missouri, Kansas City, MO 64110, USA.
authors.forename: Mathew
authors.initials: M
authors.lastname: Prideaux
authors.affiliation: Department of Oral Biology, School of Dentistry, University of Missouri, Kansas City, MO 64110, USA.
authors.forename: Lynda F
authors.initials: LF
authors.lastname: Bonewald
authors.affiliation: Department of Biochemistry, University of Wisconsin-Madison, Madison, WI 53706, USA. Electronic address: pike@biochem.wisc.edu.
authors.forename: J Wesley
authors.initials: JW
authors.lastname: Pike
mesh_terms: D016475:3T3 Cells; D000818:Animals; D001842:Bone and Bones / Q000378:metabolism; D002117:Calcitriol / Q000737:chemistry*; D002454:Cell Differentiation; D047369:Chromatin Immunoprecipitation; D017362:Cyclic AMP Response Element-Binding Protein / Q000378:metabolism; D044127:Epigenesis, Genetic; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006801:Humans; D051379:Mice; D010006:Osteoblasts / Q000166:cytology; D010011:Osteocytes / Q000166:cytology*; D010281:Parathyroid Hormone / Q000378:metabolism*; D017423:Sequence Analysis, RNA; D015398:Signal Transduction; D059467:Transcriptome
chemical_list: C115163:CREB1 protein, human; D017362:Cyclic AMP Response Element-Binding Protein; D010281:Parathyroid Hormone; D002117:Calcitriol
keywords: ChIP-seq; Osteocyte; PTH; RNA-seq; Transcriptional regulation