title: Binary release of ascorbic acid and lecithin from core-shell nanofibers on blood-contacting surface for reducing long-term hemolysis of erythrocyte.
abstract: There is an urgent need to develop blood-contacting biomaterials with long-term anti-hemolytic capability. To obtain such biomaterials, we coaxially electrospin [ascorbic acid (AA) and lecithin]/poly (ethylene oxide) (PEO) core-shell nanofibers onto the surface of styrene-b-(ethylene-co-butylene)-b-styrene elastomer (SEBS) that has been grafted with poly (ethylene glycol) (PEG) chains. Our strategy is based on that the grafted layers of PEG render the surface hydrophilic to reduce the mechanical injure to red blood cells (RBCs) while the AA and lecithin released from nanofibers on blood-contacting surface can actively interact with RBCs to decrease the oxidative damage to RBCs. We demonstrate that (AA and lecithin)/PEO core-shell structured nanofibers have been fabricated on the PEG grafted surface. The binary release of AA and lecithin in the distilled water is in a controlled manner and lasts for almost 5 days; during RBCs preservation, AA acts as an antioxidant and lecithin as a lipid supplier to the membrane of erythrocytes, resulting in low mechanical fragility and hemolysis of RBCs, as well as high deformability of stored RBCs. Our work thus makes a new approach to fabricate blood-contacting biomaterials with the capability of long-term anti-hemolysis.
authors.affiliation: State Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China. Electronic address: shiqiang@ciac.ac.cn.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Shi
authors.affiliation: Polymer Materials Research Center and Key Laboratory of Superlight Materials and Surface Technology, Ministry of Education, College of Materials Science and Chemical Engineering, Harbin Engineering University, Harbin 150001, China.
authors.forename: Qunfu
authors.initials: Q
authors.lastname: Fan
authors.affiliation: State Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Ye
authors.affiliation: State Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China.
authors.forename: Jianwen
authors.initials: J
authors.lastname: Hou
authors.affiliation: Department of Mechanical Engineering, University of Akron, Akron, OH 44325-3903, USA.
authors.forename: Shing-Chung
authors.initials: SC
authors.lastname: Wong
authors.affiliation: Polymer Materials Research Center and Key Laboratory of Superlight Materials and Surface Technology, Ministry of Education, College of Materials Science and Chemical Engineering, Harbin Engineering University, Harbin 150001, China.
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Xu
authors.affiliation: State Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China. Electronic address: yinjh@ciac.ac.cn.
authors.forename: Jinghua
authors.initials: J
authors.lastname: Yin
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000737:chemistry* / Q000494:pharmacology; D001205:Ascorbic Acid / Q000737:chemistry* / Q000494:pharmacology; D001672:Biocompatible Materials / Q000737:chemistry* / Q000494:pharmacology; D002478:Cells, Cultured; D020724:Elastomers / Q000737:chemistry / Q000494:pharmacology; D055664:Electrochemical Techniques; D004907:Erythrocyte Deformability / Q000187:drug effects; D004912:Erythrocytes / Q000166:cytology / Q000187:drug effects*; D006461:Hemolysis / Q000187:drug effects; D057927:Hydrophobic and Hydrophilic Interactions; D054709:Lecithins / Q000737:chemistry* / Q000494:pharmacology; D008855:Microscopy, Electron, Scanning; D057139:Nanofibers / Q000737:chemistry* / Q000648:ultrastructure; D011092:Polyethylene Glycols / Q000737:chemistry / Q000494:pharmacology; D011817:Rabbits; D013343:Styrenes / Q000737:chemistry / Q000494:pharmacology; D013499:Surface Properties
chemical_list: D000975:Antioxidants; D001672:Biocompatible Materials; D020724:Elastomers; D054709:Lecithins; D013343:Styrenes; D011092:Polyethylene Glycols; D001205:Ascorbic Acid
keywords: Anti-hemolysis; Ascorbic acid; Core–shell nanofiber; Erythrocyte; SEBS