title: Cytokine-mediated induction of MHC class II in human neutrophils is dependent on NADPH oxidase activity.
abstract: In response to selected cytokines, neutrophils are induced to express MHC class II and acquire properties of antigen-presenting cells. Here we show that (a) GM-CSF- and IFN-γ-mediated induction of MHC class II in human neutrophils is associated with intracellular ROS up-regulation, (b) cell-permeable ROS scavengers MnTMPyP and polyethylene glycol-conjugated superoxide dismutase and NADPH oxidase inhibitors diphenylene iodonium and apocynin abrogate both the cytokine-mediated ROS elevation and the induction of MHC class II and (c) neutrophils from chronic granulomatous disease patients which lack NADPH oxidase activity fail to express MHC class II in response to the cytokines. Thus, NADPH oxidase activity is required for the cytokine-mediated induction of MHC class II expression in neutrophils.
authors.affiliation: Hematology Research Center, Novy Zykovsky Pr. 4, Moscow 125167, Russia. Electronic address: bpliyev@cardio.ru.
authors.forename: Boris K
authors.initials: BK
authors.lastname: Pliyev
authors.affiliation: Hematology Research Center, Novy Zykovsky Pr. 4, Moscow 125167, Russia.
authors.forename: Tatyana V
authors.initials: TV
authors.lastname: Dimitrieva
authors.affiliation: Hematology Research Center, Novy Zykovsky Pr. 4, Moscow 125167, Russia.
authors.forename: Valery G
authors.initials: VG
authors.lastname: Savchenko
mesh_terms: D002478:Cells, Cultured; D016207:Cytokines / Q000276:immunology* / Q000378:metabolism; D004789:Enzyme Activation; D000949:Histocompatibility Antigens Class II / Q000276:immunology* / Q000378:metabolism; D006801:Humans; D019255:NADPH Oxidases / Q000276:immunology* / Q000378:metabolism; D009504:Neutrophils / Q000201:enzymology / Q000276:immunology*; D017382:Reactive Oxygen Species / Q000276:immunology / Q000378:metabolism
chemical_list: D016207:Cytokines; D000949:Histocompatibility Antigens Class II; D017382:Reactive Oxygen Species; D019255:NADPH Oxidases
keywords: MHC class II; NADPH oxidase; Neutrophils; ROS