title: Non-host disease resistance response in pea (Pisum sativum) pods: Biochemical function of DRR206 and phytoalexin pathway localization.
abstract: Continually exposed to potential pathogens, vascular plants have evolved intricate defense mechanisms to recognize encroaching threats and defend themselves. They do so by inducing a set of defense responses that can help defeat and/or limit effects of invading pathogens, of which the non-host disease resistance response is the most common. In this regard, pea (Pisum sativum) pod tissue, when exposed to Fusarium solani f. sp. phaseoli spores, undergoes an inducible transcriptional activation of pathogenesis-related genes, and also produces (+)-pisatin, its major phytoalexin. One of the inducible pathogenesis-related genes is Disease Resistance Response-206 (DRR206), whose role in vivo was unknown. DRR206 is, however, related to the dirigent protein (DP) family. In this study, its biochemical function was investigated in planta, with the metabolite associated with its gene induction being pinoresinol monoglucoside. Interestingly, both pinoresinol monoglucoside and (+)-pisatin were co-localized in pea pod endocarp epidermal cells, as demonstrated using matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging. In addition, endocarp epidermal cells are also the site for both chalcone synthase and DRR206 gene expression. Taken together, these data indicate that both (+)-pisatin and pinoresinol monoglucoside function in the overall phytoalexin responses.
authors.affiliation: Institute of Biological Chemistry, Washington State University, Pullman, WA 99164-6340, USA.
authors.forename: Herana Kamal
authors.initials: HK
authors.lastname: Seneviratne
authors.affiliation: Institute of Biological Chemistry, Washington State University, Pullman, WA 99164-6340, USA.
authors.forename: Doralyn S
authors.initials: DS
authors.lastname: Dalisay
authors.affiliation: Institute of Biological Chemistry, Washington State University, Pullman, WA 99164-6340, USA.
authors.forename: Kye-Won
authors.initials: KW
authors.lastname: Kim
authors.affiliation: Institute of Biological Chemistry, Washington State University, Pullman, WA 99164-6340, USA.
authors.forename: Syed G A
authors.initials: SG
authors.lastname: Moinuddin
authors.affiliation: Institute of Biological Chemistry, Washington State University, Pullman, WA 99164-6340, USA.
authors.forename: Hong
authors.initials: H
authors.lastname: Yang
authors.affiliation: Institute of Biological Chemistry, Washington State University, Pullman, WA 99164-6340, USA.
authors.forename: Christopher M
authors.initials: CM
authors.lastname: Hartshorn
authors.affiliation: Institute of Biological Chemistry, Washington State University, Pullman, WA 99164-6340, USA.
authors.forename: Laurence B
authors.initials: LB
authors.lastname: Davin
authors.affiliation: Institute of Biological Chemistry, Washington State University, Pullman, WA 99164-6340, USA. Electronic address: lewisn@wsu.edu.
authors.forename: Norman G
authors.initials: NG
authors.lastname: Lewis
mesh_terms: D060467:Disease Resistance / Q000235:genetics; D005663:Furans / Q000378:metabolism; D005786:Gene Expression Regulation; D017705:Lignans / Q000378:metabolism; D015394:Molecular Structure; D018532:Pisum sativum / Q000737:chemistry / Q000166:cytology / Q000235:genetics / Q000378:metabolism; D010935:Plant Diseases / Q000235:genetics; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D036343:Pterocarpans / Q000737:chemistry / Q000378:metabolism; D012717:Sesquiterpenes / Q000737:chemistry / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D000094162:Phytoalexins
chemical_list: C000604000:DRR206 protein, Pisum sativum; D005663:Furans; D017705:Lignans; D010940:Plant Proteins; D036343:Pterocarpans; D012717:Sesquiterpenes; C103298:pinoresinol; C003161:pisatin; D000094162:Phytoalexins
keywords: Dirigent protein; Fabaceae; Fusarium solani f. sp. phaseoli; Gene expression; MALDI mass spectrometry imaging; Non-host disease response; Pea; Phytoalexin; Pinoresinol; Pinoresinol monoglucoside; Pisatin; Pisum sativum