title: Past, present, and future perspectives on the diagnosis of Roseolovirus infections.
abstract: Diagnosis of Roseolovirus infections mandates careful selection of patients, samples, and testing methods. We review advances in the field and highlight research priorities. Quantitative (q)PCR can accurately identify and distinguish between human herpesvirus 6 (HHV-6) species A and B. Whether screening of high-risk patients improves outcomes is unclear. Chromosomally integrated (ci)HHV-6 confounds test interpretation but can be ruled out with digital PCR. Reverse transcription qPCR may be a more specific and clinically applicable test for actively replicating Roseoloviruses, particularly among patients with ciHHV-6. Interpretation of Roseolovirus test results faces many challenges. However, careful application of refined and emerging diagnostic techniques will allow for increasingly accurate diagnosis of clinically significant infections and disease associations.
authors.affiliation: Department of Medicine, University of Washington, Seattle, WA, United States; Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Research Center, Seattle, WA, United States.
authors.forename: Joshua A
authors.initials: JA
authors.lastname: Hill
authors.affiliation: Department of Laboratory Medicine, University of Washington, Seattle, WA, United States.
authors.forename: Ruth Hall
authors.initials: RH
authors.lastname: Sedlak
authors.affiliation: Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Research Center, Seattle, WA, United States; Department of Laboratory Medicine, University of Washington, Seattle, WA, United States. Electronic address: kjerome@u.washington.edu.
authors.forename: Keith R
authors.initials: KR
authors.lastname: Jerome
mesh_terms: D003955:Diagnostic Tests, Routine / Q000379:methods* / Q000639:trends; D006801:Humans; D025202:Molecular Diagnostic Techniques / Q000379:methods* / Q000639:trends; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D019211:Roseolovirus / Q000235:genetics / Q000302:isolation & purification*; D019349:Roseolovirus Infections / Q000175:diagnosis*; D014773:Virology / Q000379:methods* / Q000639:trends