title: Capture and Release (CaR): a simplified procedure for one-tube isolation and concentration of single-stranded DNA during SELEX.
abstract: Short biotinylated oligodeoxynucleotides immobilized on streptavidin-coated magnetic beads allow for convenient and rapid purification of single-stranded oligodeoxynucleotides from crude asymmetric PCR mixtures, facilitating the selection of DNA aptamers.
authors.affiliation: Institute for Exp. Haematology and Transfusion Medicine, University of Bonn Medical Centre, Bonn, Germany. jens.mueller@ukb.uni-bonn.de.
authors.forename: Nasim Shahidi
authors.initials: NS
authors.lastname: Hamedani
authors.forename: Fabian
authors.initials: F
authors.lastname: Blümke
authors.forename: Fabian
authors.initials: F
authors.lastname: Tolle
authors.forename: Falk
authors.initials: F
authors.lastname: Rohrbach
authors.forename: Heiko
authors.initials: H
authors.lastname: Rühl
authors.forename: Johannes
authors.initials: J
authors.lastname: Oldenburg
authors.forename: Günter
authors.initials: G
authors.lastname: Mayer
authors.forename: Bernd
authors.initials: B
authors.lastname: Pötzsch
authors.forename: Jens
authors.initials: J
authors.lastname: Müller
mesh_terms: D004277:DNA, Single-Stranded / Q000032:analysis*; D005821:Genetic Techniques; D052156:SELEX Aptamer Technique
chemical_list: D004277:DNA, Single-Stranded