title: Ag nanoclusters as probes for turn-on fluorescence recognition of TpG dinucleotide with a high selectivity.
abstract: CpG dinucleotide in DNA has a great tendency to mutate to TpG dinucleotide and this transition can cause some serious diseases. In this work, fluorescent Ag nanoclusters (Ag NCs) were employed as useful inorganic fluorophores for the potential of selectively discriminating TpG dinucleotide from CpG dinucleotide. Opposite the base Y of interest in YpG dinucleotide (Y=C or T), a bulge site was introduced so as to make the base Y to be unpaired and ready for Ag(+) binding. Such that the unpaired Y and context base pairs can provide a specific space suitable for creating fluorescent Ag NCs. We found that in comparison with CpG dinucleotide, TpG dinucleotide is much more efficient in growing fluorescent Ag NCs. Therefore, mutation of CpG dinucleotide to TpG can be identified by a turn-on fluorescence response and a high selectivity. More interestingly, Ag NCs exhibit a better performance in the TpG recognition over the other dinucleotides (Y=A and G) than the previously used organic fluorophores. Additionally, the effectiveness of the bulge site design in discriminating these dinucleotides was evidenced by control DNAs having the abasic site structure. We expect that a practical method for TpG dinucleotide recognition with a high selectivity can be developed using the bulge site-grown fluorescent Ag NCs as novel probes.
authors.affiliation: Institute of Physical Chemistry, Zhejiang Normal University, Jinhua, Zhejiang 321004, PR China.
authors.forename: Jian
authors.initials: J
authors.lastname: Peng
authors.affiliation: Institute of Physical Chemistry, Zhejiang Normal University, Jinhua, Zhejiang 321004, PR China. Electronic address: yshao@zjnu.cn.
authors.forename: Yong
authors.initials: Y
authors.lastname: Shao
authors.affiliation: Institute of Physical Chemistry, Zhejiang Normal University, Jinhua, Zhejiang 321004, PR China.
authors.forename: Lingling
authors.initials: L
authors.lastname: Liu
authors.affiliation: Institute of Physical Chemistry, Zhejiang Normal University, Jinhua, Zhejiang 321004, PR China.
authors.forename: Lihua
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Institute of Physical Chemistry, Zhejiang Normal University, Jinhua, Zhejiang 321004, PR China.
authors.forename: Hua
authors.initials: H
authors.lastname: Liu
authors.affiliation: Institute of Physical Chemistry, Zhejiang Normal University, Jinhua, Zhejiang 321004, PR China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Wang
mesh_terms: D020029:Base Pairing; D001483:Base Sequence; D004247:DNA / Q000737:chemistry*; D015226:Dinucleoside Phosphates / Q000032:analysis*; D005456:Fluorescent Dyes / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry*; D012834:Silver / Q000737:chemistry*; D013050:Spectrometry, Fluorescence
chemical_list: D015226:Dinucleoside Phosphates; D005456:Fluorescent Dyes; D012834:Silver; C077117:thymidylyl-(3',5')-2'-deoxyguanosine; D004247:DNA
keywords: Dinucleotide mutation; Fluorescence; Nucleic acid; Selectivity; Silver nanoclusters