title: Construction of an efficient RNAi system in the cellulolytic fungus Trichoderma reesei.
abstract: An improved RNA interference method was developed in Trichoderma reesei, using convergent dual promoters for efficient and high-throughput RNA silencing. This new vector allowed for the silencing of the eGFP gene and target genes to occur simultaneously, significantly facilitating the rapid screening of the transformants using eGFP as a reporter.
authors.affiliation: Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, 32 West 7th Avenue, Tianjin Airport Economic Area, Tianjin 300308, PR China; Key Laboratory of Industrial Fermentation Microbiology (Tianjin University of Science & Technology), Ministry of Education, Tianjin 300457, PR China; Tianjin Key Lab of Industrial Microbiology, Tianjin University of Science and Technology, Tianjin 300457, PR China.
authors.forename: Ronglin
authors.initials: R
authors.lastname: He
authors.affiliation: Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, 32 West 7th Avenue, Tianjin Airport Economic Area, Tianjin 300308, PR China.
authors.forename: Wei
authors.initials: W
authors.lastname: Guo
authors.affiliation: Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, 32 West 7th Avenue, Tianjin Airport Economic Area, Tianjin 300308, PR China.
authors.forename: Lixian
authors.initials: L
authors.lastname: Wang
authors.affiliation: Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, 32 West 7th Avenue, Tianjin Airport Economic Area, Tianjin 300308, PR China. Electronic address: zhang_dy@tib.cas.cn.
authors.forename: Dongyuan
authors.initials: D
authors.lastname: Zhang
mesh_terms: D002482:Cellulose / Q000378:metabolism; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism; D018390:Gene Targeting / Q000379:methods*; D011401:Promoter Regions, Genetic; D034622:RNA Interference; D014170:Transformation, Genetic; D014242:Trichoderma / Q000235:genetics* / Q000378:metabolism
chemical_list: D005656:Fungal Proteins; D002482:Cellulose
keywords: Dual promoter; RNAi; Trichoderma reesei; eGFP