title: Assessment of colloid response by nonlinear optical microscopy after preoperative radiochemotherapy for rectal carcinoma.
abstract: Colloid response is a type of tumor response that occurs after preoperative radiochemotherapy for rectal carcinoma. Given its important influence on survival, the colloid response should be considered when estimating histopathological reactions. Here, multiphoton microscopy (MPM) was applied to evaluate the colloid response ex vivo. This study demonstrated that MPM has the capability to visualize the colloid response in the absence of labels and can, in particular, identify rare residual carcinomatous cells in mucin pools. These results highlight the potential of this nonlinear optical technique as a diagnostic tool for tumor response after neoadjuvant treatment.
authors.affiliation: Institute of Laser and Optoelectronics Technology, Fujian Provincial Key Laboratory for Photonics Technology, Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, Fujian Normal University, Fuzhou 350007, China.
authors.forename: Lianhuang
authors.initials: L
authors.lastname: Li
authors.affiliation: Fujian Medical University, The Affiliated Union Hospital, Department of Colorectal Surgery, Fuzhou 350001, China.
authors.forename: Zhifen
authors.initials: Z
authors.lastname: Chen
authors.affiliation: Fujian Medical University, The First Affiliated Hospital, Department of Pathology, Fuzhou 350001, China.
authors.forename: Xingfu
authors.initials: X
authors.lastname: Wang
authors.affiliation: Institute of Laser and Optoelectronics Technology, Fujian Provincial Key Laboratory for Photonics Technology, Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, Fujian Normal University, Fuzhou 350007, China.
authors.forename: Shuangmu
authors.initials: S
authors.lastname: Zhuo
authors.affiliation: Institute of Laser and Optoelectronics Technology, Fujian Provincial Key Laboratory for Photonics Technology, Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, Fujian Normal University, Fuzhou 350007, China.
authors.forename: Hongsheng
authors.initials: H
authors.lastname: Li
authors.affiliation: Fujian Medical University, The Affiliated Union Hospital, Department of Colorectal Surgery, Fuzhou 350001, China.
authors.forename: Weizhong
authors.initials: W
authors.lastname: Jiang
authors.affiliation: Fujian Medical University, The Affiliated Union Hospital, Department of Colorectal Surgery, Fuzhou 350001, China.
authors.forename: Guoxian
authors.initials: G
authors.lastname: Guan
authors.affiliation: Institute of Laser and Optoelectronics Technology, Fujian Provincial Key Laboratory for Photonics Technology, Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, Fujian Normal University, Fuzhou 350007, China.
authors.forename: Jianxin
authors.initials: J
authors.lastname: Chen
mesh_terms: D002277:Carcinoma / Q000188:drug therapy* / Q000532:radiotherapy*; D059248:Chemoradiotherapy; D003102:Colloids / Q000737:chemistry*; D006801:Humans; D008853:Microscopy; D009077:Mucins / Q000737:chemistry; D020360:Neoadjuvant Therapy; D055095:Optics and Photonics; D012004:Rectal Neoplasms / Q000188:drug therapy* / Q000532:radiotherapy*; D012007:Rectum / Q000473:pathology; D012008:Recurrence
chemical_list: D003102:Colloids; D009077:Mucins