title: Self-assembly of β-lactoglobulin and egg white lysozyme as a potential carrier for nutraceuticals.
abstract: Self-assembly structures of β-lactoglobulin (βlg) and egg protein lysozyme (Lyso) were developed, using electrostatic interactions between the two oppositely charged proteins. Different βlg/Lyso concentration ratios were essayed at pH 6.8 to select the optimal ratio for the proteins co-precipitation, which behaviour was then studied at varying pH values. Optimal βlg/Lyso concentration ratio, prepared at pH 7.5, was selected for protein co-precipitation. As a result, a structure with a mean diameter of 7.1±2.5 μm was formed, as indicated by static light scattering. Furthermore, the SEM images showed that βlg and Lyso self-assembled to form a microsphere. Vitamin D3, used as a model nutraceutical, was successfully entrapped in the βlg/Lyso microspheres with an encapsulation efficiency of 90.8±4.8%. Therefore, the βlg/Lyso microspheres can serve as a potential food-grade vehicle for bioactives in the formulation of food products and pharmaceuticals.
authors.affiliation: Chaire de recherche du Canada sur les protéines, les bio-systèmes et les aliments fonctionnels, Institut de recherche sur les nutraceutiques et les aliments fonctionnels (INAF/STELA), Université Laval, Québec, QC, Canada.
authors.forename: Fatoumata
authors.initials: F
authors.lastname: Diarrassouba
authors.affiliation: Centre de recherche et développement, Agropur Coopérative. 4700 rue Armand Frappier, St-Hubert, QC, Canada.
authors.forename: Gabriel
authors.initials: G
authors.lastname: Remondetto
authors.affiliation: EA-CIDAM, Lab Biopharmacie, Faculté de Pharmacie, 28 Place Henri Dunant, 63001 Clermont-Ferrand, France.
authors.forename: Ghislain
authors.initials: G
authors.lastname: Garrait
authors.affiliation: Chaire de recherche du Canada sur les protéines, les bio-systèmes et les aliments fonctionnels, Institut de recherche sur les nutraceutiques et les aliments fonctionnels (INAF/STELA), Université Laval, Québec, QC, Canada.
authors.forename: Pedro
authors.initials: P
authors.lastname: Alvarez
authors.affiliation: EA-CIDAM, Lab Biopharmacie, Faculté de Pharmacie, 28 Place Henri Dunant, 63001 Clermont-Ferrand, France.
authors.forename: Eric
authors.initials: E
authors.lastname: Beyssac
authors.affiliation: Chaire de recherche du Canada sur les protéines, les bio-systèmes et les aliments fonctionnels, Institut de recherche sur les nutraceutiques et les aliments fonctionnels (INAF/STELA), Université Laval, Québec, QC, Canada. Electronic address: Muriel.Subirade@fsaa.ulaval.ca.
authors.forename: Muriel
authors.initials: M
authors.lastname: Subirade
mesh_terms: D002762:Cholecalciferol / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D019587:Dietary Supplements; D004337:Drug Carriers; D004529:Egg White / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D007782:Lactoglobulins / Q000737:chemistry*; D008863:Microspheres; D009113:Muramidase / Q000737:chemistry*
chemical_list: D004337:Drug Carriers; D007782:Lactoglobulins; D002762:Cholecalciferol; D009113:Muramidase
keywords: Bioactives; Delivery vehicle; Lysozyme; Proteins; Self-assembly; β-Lactoglobulin