title: Gold nanocluster-based fluorescence biosensor for targeted imaging in cancer cells and ratiometric determination of intracellular pH.
abstract: The dysregulated pH is working as a mark of cancer. It is a challenge for developing a biosensor for targeted imaging in cancer cells and monitoring of intracellular pH. Here, a ratiometric fluorescence biosensor for pH determination was developed with targeted imaging into folate acceptor (FR)-rich cancer cells at the same time. AuNCs protected by bovine serum albumin (BSA) worked as reference fluorophore and fluorescein-isothiocyanate (FITC) acted as the response signal for pH. For targeted imaging of cancer cells, the AuNCs were simultaneously conjugated with folic acid (FA). The developed ratiometric biosensor can monitor pH with a wide linear range from 6.0-7.8 with a pKa at 6.84. Under every different pH condition, the probe showed high selectivity over various metal ions and amino acids with its fluorescence ratio stayed almost constant (<5%). It also showed good cyclic accuracy when pH switched between 6.0 and 8.0, as well as low cytotoxicity. The AuNC-based inorganic-organic nanohybrid biosensor showed good cell-permeability, low cytotoxicity, and long-term photostability. Accordingly, the pH biosensor was employed to gain targeted imaging in FR(+ve) Hela cells with FR(-ve) lung carcinoma cells A549 as comparison, and achieved to monitor the pH changes in Hela cells.
authors.affiliation: Department of Chemistry, Tongji University, Siping Road 1239, Shanghai 200092, People's Republic of China.
authors.forename: Changqin
authors.initials: C
authors.lastname: Ding
authors.affiliation: Department of Chemistry, Tongji University, Siping Road 1239, Shanghai 200092, People's Republic of China; Department of Chemistry, East China Normal University, North Zhongshan Road 3663, Shanghai 200062, People's Republic of China. Electronic address: ytian@chem.ecnu.edu.cn.
authors.forename: Yang
authors.initials: Y
authors.lastname: Tian
mesh_terms: D000072283:A549 Cells; D015374:Biosensing Techniques / Q000379:methods*; D016650:Fluorescein-5-isothiocyanate / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry*; D058973:Folate Receptors, GPI-Anchored / Q000032:analysis*; D005492:Folic Acid / Q000737:chemistry; D006046:Gold / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D006863:Hydrogen-Ion Concentration; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D009369:Neoplasms / Q000000981:diagnostic imaging*; D061848:Optical Imaging / Q000379:methods*; D013050:Spectrometry, Fluorescence / Q000379:methods
chemical_list: D005456:Fluorescent Dyes; D058973:Folate Receptors, GPI-Anchored; D006046:Gold; D005492:Folic Acid; D016650:Fluorescein-5-isothiocyanate
keywords: Au nanoclusters; Cancer cells; Folate receptor; Ratiometric fluorescence biosensor; Targeted imaging; pH determination