title: C/EBPα controls mast cell function.
abstract: CCAAT/enhancer binding protein alpha (C/EBPα) is a transcription factor that influences immune cell fate and differentiation. However, the effect of C/EBPα on mast cells is not fully understood. In this study, we showed that C/EBPα suppressed granule formation in mast cells and increased macrophage inflammatory protein (MIP)-2 production from mast cells upon bacterial stimulation. These results indicate that C/EBPα regulates the balance between the allergic response and the innate immune response of mast cells. Furthermore, we showed that stimulation of mast cells with the Lactobacillus casei JCM1134(T) strain during late differentiation up-regulated C/EBPα expression in differentiated mast cells. This suggests that intestinal commensal bacteria modulate C/EBPα expression and thereby regulate mast cell function.
authors.affiliation: College of Bioresource Sciences, Nihon University, Fujisawa, Kanagawa, Japan; Atopy (Allergy) Research Center, Juntendo University School of Medicine, Bunkyo, Tokyo, Japan; Department of Biological Science and Technology, Tokyo University of Science, Katsushika, Tokyo, Japan.
authors.forename: Kazumi
authors.initials: K
authors.lastname: Kasakura
authors.affiliation: College of Bioresource Sciences, Nihon University, Fujisawa, Kanagawa, Japan. Electronic address: ktaka@brs.nihon-u.ac.jp.
authors.forename: Kyoko
authors.initials: K
authors.lastname: Takahashi
authors.affiliation: College of Bioresource Sciences, Nihon University, Fujisawa, Kanagawa, Japan.
authors.forename: Tomoko
authors.initials: T
authors.lastname: Itoh
authors.affiliation: College of Bioresource Sciences, Nihon University, Fujisawa, Kanagawa, Japan.
authors.forename: Akira
authors.initials: A
authors.lastname: Hosono
authors.affiliation: Department of Molecular Cell Immunology and Allergology, Nihon University School of Medicine, Itabashi, Tokyo, Japan.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Nunomura
authors.affiliation: Department of Molecular Cell Immunology and Allergology, Nihon University School of Medicine, Itabashi, Tokyo, Japan.
authors.forename: Chisei
authors.initials: C
authors.lastname: Ra
authors.affiliation: Department of Veterinary Public Health, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo, Tokyo, Japan.
authors.forename: Yoshika
authors.initials: Y
authors.lastname: Momose
authors.affiliation: Department of Veterinary Public Health, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo, Tokyo, Japan.
authors.forename: Kikuji
authors.initials: K
authors.lastname: Itoh
authors.affiliation: Atopy (Allergy) Research Center, Juntendo University School of Medicine, Bunkyo, Tokyo, Japan; Department of Biological Science and Technology, Tokyo University of Science, Katsushika, Tokyo, Japan.
authors.forename: Chiharu
authors.initials: C
authors.lastname: Nishiyama
authors.affiliation: College of Bioresource Sciences, Nihon University, Fujisawa, Kanagawa, Japan.
authors.forename: Shuichi
authors.initials: S
authors.lastname: Kaminogawa
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology; D022763:CCAAT-Enhancer-Binding Protein-alpha / Q000235:genetics / Q000378:metabolism*; D002454:Cell Differentiation; D054426:Chemokine CXCL2 / Q000096:biosynthesis; D005260:Female; D005786:Gene Expression Regulation; D007780:Lacticaseibacillus casei / Q000502:physiology; D008407:Mast Cells / Q000166:cytology* / Q000276:immunology / Q000378:metabolism* / Q000382:microbiology; D051379:Mice
chemical_list: D022763:CCAAT-Enhancer-Binding Protein-alpha; D054426:Chemokine CXCL2; C404915:Cxcl2 protein, mouse
keywords: Allergy; CCAAT/enhancer binding protein alpha; Commensal bacteria; Innate immunity; Mast cell; Terminal differentiation