title: Response of direct or priming defense against Botrytis cinerea to methyl jasmonate treatment at different concentrations in grape berries.
abstract: This study was conducted to characterize the forms of disease resistance induced by methyl jasmonate (MeJA) in harvested grape berries and to evaluate the impact of the induced resistance on fruit quality. The results showed that MeJA treatment at concentrations from 10 to 100μmol/L could effectively induce disease resistance against Botrytis cinerea and reduce disease incidence in grape berries. The induced disease resistance was tightly associated with increased H2O2 generation, enhanced expression of the defense-related gene VvNPR1.1 and accumulation of stilbene phytoalexins such as tran-resveratrol and its oligomer (trans-)ε-viniferin. The expression of the defense-related gene and synthesis of phytoalexins in 10μmol/L MeJA-treated grape berries were only significantly enhanced upon inoculating the berries with B. cinerea, whereas the 50 or 100μmol/L of MeJA treatment directly induced these defense responses. Hence, we deduce that the low concentration of MeJA (10μmol/L) triggered a priming defense mechanism, while higher concentrations of MeJA (50 or 100μmol/L) directly activated defense responses, thus enhancing disease resistance in grape berries. Moreover, the primed grape berries maintained higher contents of soluble sugars and higher DPPH radical scavenging activity and reducing power compared with those expressing direct defense responses. These results indicate that priming of defense is a cost-effective strategy to protect harvested grape berries from B. cinerea infection in terms of minimizing quality loss.
authors.affiliation: College of Life Science and Engineering, Chongqing Three Gorges University, Chongqing 404100, PR China; College of Food Science, Southwest University, Chongqing 400715, PR China; College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Kaituo
authors.initials: K
authors.lastname: Wang
authors.affiliation: College of Life Science and Engineering, Chongqing Three Gorges University, Chongqing 404100, PR China.
authors.forename: Yunxia
authors.initials: Y
authors.lastname: Liao
authors.affiliation: College of Food Science, Southwest University, Chongqing 400715, PR China.
authors.forename: Jianquan
authors.initials: J
authors.lastname: Kan
authors.affiliation: College of Food Science, Southwest University, Chongqing 400715, PR China.
authors.forename: Lin
authors.initials: L
authors.lastname: Han
authors.affiliation: College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, PR China. Electronic address: zhengyh@njau.edu.cn.
authors.forename: Yonghua
authors.initials: Y
authors.lastname: Zheng
mesh_terms: D000085:Acetates / Q000494:pharmacology*; D001572:Benzofurans / Q000378:metabolism; D020171:Botrytis / Q000502:physiology*; D003517:Cyclopentanes / Q000494:pharmacology*; D060467:Disease Resistance / Q000187:drug effects*; D005638:Fruit / Q000382:microbiology; D005786:Gene Expression Regulation / Q000187:drug effects; D006861:Hydrogen Peroxide / Q000378:metabolism; D054883:Oxylipins / Q000494:pharmacology*; D010937:Plant Growth Regulators / Q000494:pharmacology; D000077185:Resveratrol; D013267:Stilbenes / Q000378:metabolism; D027843:Vitis / Q000187:drug effects* / Q000382:microbiology*
chemical_list: D000085:Acetates; D001572:Benzofurans; D003517:Cyclopentanes; D054883:Oxylipins; D010937:Plant Growth Regulators; D013267:Stilbenes; C091891:epsilon-viniferin; C072239:methyl jasmonate; D006861:Hydrogen Peroxide; D000077185:Resveratrol
keywords: Defense priming; Grape berries; Induced disease resistance; Methyl jasmonate; Quality