title: Secondhand smoke exposure-induced nucleocytoplasmic shuttling of HMGB1 in a rat premature skin aging model.
abstract: Secondhand cigarette smoke exposure (SSE) has been linked to carcinogenic, oxidative, and inflammatory reactions. Herein, we investigated whether premature skin aging could be induced by SSE in a rat model, and assessed the cytoplasmic translocation of high mobility group box 1 (HMGB1) protein and collagen loss in skin tissues. Animals were divided into two groups: SSE and controls. Whole body SSE was carried out for 12 weeks. Dorsal skin tissue specimens were harvested for HMGB1 and Mallory's azan staining. Correlations between serum HMGB1 and collagen levels were determined. Rat skin exposed to secondhand smoke lost collagen bundles in the papillary dermis and collagen decreased significantly (p<0.05) compared with control rats. In epidermal keratinocytes, cytoplasmic HMGB1 staining was more diffuse and there were more HMGB1-positive cells after four weeks in SSE compared to control rats. A negative correlation between HMGB1 serum and collagen levels (r=-0.631, p=0.28) was also observed. Therefore, cytoplasmic HMGB1 expression in skin tissues might be associated with skin collagen loss upon the initiation of SSE. Additionally, long-term SSE might affect the appearance of the skin, or could accelerate the skin aging process.
authors.affiliation: School of Anti-Aging and Regenerative Medicine, Mae Fah Luang University, Bangkok 10110, Thailand.
authors.forename: Sirintip
authors.initials: S
authors.lastname: Chaichalotornkul
authors.affiliation: School of Anti-Aging and Regenerative Medicine, Mae Fah Luang University, Bangkok 10110, Thailand.
authors.forename: Thamthiwat
authors.initials: T
authors.lastname: Nararatwanchai
authors.affiliation: Department of Pathobiology, Faculty of Science, Mahidol University, Bangkok 10400, Thailand.
authors.forename: Somphong
authors.initials: S
authors.lastname: Narkpinit
authors.affiliation: Department of Pharmacology, Faculty of Dentistry, Mahidol University, Bangkok 10400, Thailand.
authors.forename: Pornpen
authors.initials: P
authors.lastname: Dararat
authors.affiliation: Department of Pharmacology, Faculty of Dentistry, Mahidol University, Bangkok 10400, Thailand; Division of Brain Science, Department of Physiology, Kurume University School of Medicine, Fukuoka 830-0011, Japan.
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Kikuchi
authors.affiliation: Department of Systems Biology in Thromboregulation, Kagoshima University Graduate School of Medical and Dental Sciences, Kagoshima 890-8520, Japan.
authors.forename: Ikuro
authors.initials: I
authors.lastname: Maruyama
authors.affiliation: Department of Pharmacology, Faculty of Dentistry, Mahidol University, Bangkok 10400, Thailand. Electronic address: salunya.tan@mahidol.ac.th.
authors.forename: Salunya
authors.initials: S
authors.lastname: Tancharoen
mesh_terms: D019588:Aging, Premature / Q000473:pathology; D000818:Animals; D001835:Body Weight / Q000187:drug effects; D002467:Cell Nucleus / Q000378:metabolism*; D003094:Collagen / Q000378:metabolism; D003367:Cotinine / Q000737:chemistry; D003593:Cytoplasm / Q000378:metabolism*; D024243:HMGB1 Protein / Q000378:metabolism*; D007150:Immunohistochemistry; D015603:Keratinocytes / Q000166:cytology; D008297:Male; D009538:Nicotine / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar; D012867:Skin / Q000187:drug effects*; D015595:Skin Aging / Q000473:pathology*; D013997:Time Factors; D014028:Tobacco Smoke Pollution / Q000009:adverse effects*
chemical_list: D024243:HMGB1 Protein; C104200:Hbp1 protein, rat; D014028:Tobacco Smoke Pollution; D009538:Nicotine; D003094:Collagen; D003367:Cotinine
keywords: Collagen; Cotinine; HMGB1; Nicotine; Premature skin aging; Secondhand smoke