title: Atypical nuclear localization of VIP receptors in glioma cell lines and patients.
abstract: An increasing number of G protein-coupled receptors, like receptors for vasoactive intestinal peptide (VIP), are found in cell nucleus. As VIP receptors are involved in the regulation of glioma cell proliferation and migration, we investigated the expression and the nuclear localization of the VIP receptors VPAC1 and VPAC2 in this cancer. First, by applying Western blot and immunofluorescence detection in three human glioblastoma (GBM) cell lines, we observed a strong nuclear staining for the VPAC1 receptor and a weak nuclear VPAC2 receptor staining. Second, immunohistochemical staining of VPAC1 and VPAC2 on tissue microarrays (TMA) showed that the two receptors were expressed in normal brain and glioma tissues. Expression in the non-nuclear compartment of the two receptors significantly increased with the grade of the tumors. Analysis of nuclear staining revealed a significant increase of VPAC1 staining with glioma grade, with up to 50% of GBM displaying strong VPAC1 nuclear staining, whereas nuclear VPAC2 staining remained marginal. The increase in VPAC receptor expression with glioma grades and the enhanced nuclear localization of the VPAC1 receptors in GBM might be of importance for glioma progression.
authors.affiliation: Equipe Récepteurs, Régulations et Cellules Tumorales, Université de Poitiers, PBS bât 36, 1 rue Georges Bonnet, TSA 51106, 86073 Poitiers Cedex 9, France.
authors.forename: Alice
authors.initials: A
authors.lastname: Barbarin
authors.affiliation: Equipe Récepteurs, Régulations et Cellules Tumorales, Université de Poitiers, PBS bât 36, 1 rue Georges Bonnet, TSA 51106, 86073 Poitiers Cedex 9, France.
authors.forename: Paule
authors.initials: P
authors.lastname: Séité
authors.affiliation: Laboratoire d'anatomie et de cytologie pathologiques, CHU de Poitiers, 2 rue de la Milétrie, 86000 Poitiers, France.
authors.forename: Julie
authors.initials: J
authors.lastname: Godet
authors.affiliation: Equipe Récepteurs, Régulations et Cellules Tumorales, Université de Poitiers, PBS bât 36, 1 rue Georges Bonnet, TSA 51106, 86073 Poitiers Cedex 9, France.
authors.forename: Souheyla
authors.initials: S
authors.lastname: Bensalma
authors.affiliation: Equipe Récepteurs, Régulations et Cellules Tumorales, Université de Poitiers, PBS bât 36, 1 rue Georges Bonnet, TSA 51106, 86073 Poitiers Cedex 9, France.
authors.forename: Jean-Marc
authors.initials: JM
authors.lastname: Muller
authors.affiliation: Equipe Récepteurs, Régulations et Cellules Tumorales, Université de Poitiers, PBS bât 36, 1 rue Georges Bonnet, TSA 51106, 86073 Poitiers Cedex 9, France. Electronic address: corinne.chadeneau@univ-poitiers.fr.
authors.forename: Corinne
authors.initials: C
authors.lastname: Chadéneau
mesh_terms: D021581:Active Transport, Cell Nucleus; D000293:Adolescent; D000328:Adult; D000368:Aged; D002467:Cell Nucleus / Q000378:metabolism*; D002648:Child; D005260:Female; D005910:Glioma / Q000473:pathology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D051239:Receptors, Vasoactive Intestinal Peptide, Type II / Q000032:analysis* / Q000378:metabolism; D051238:Receptors, Vasoactive Intestinal Polypeptide, Type I / Q000032:analysis* / Q000378:metabolism; D046888:Tissue Array Analysis; D014407:Tumor Cells, Cultured; D055815:Young Adult
chemical_list: D051239:Receptors, Vasoactive Intestinal Peptide, Type II; D051238:Receptors, Vasoactive Intestinal Polypeptide, Type I
keywords: GPCR; Glioblastoma; Glioma; Nuclear localization; Tissue microarrays; VIP receptors