title: Acaricidal activity of petroleum ether extract of leaves of Tetrastigma leucostaphylum (Dennst.) Alston against Rhipicephalus (Boophilus) annulatus.
abstract: The acaricidal activity of the petroleum ether extract of leaves of Tetrastigma leucostaphylum (Dennst.) Alston (family: Vitaceae) against Rhipicephalus (Boophilus) annulatus was assessed using adult immersion test (AIT). The per cent of adult mortality, inhibition of fecundity, and blocking of hatching of eggs were studied at different concentrations. The extract at 10% concentration showed 88.96% inhibition of fecundity, 58.32% of adult tick mortality, and 50% inhibition of hatching. Peak mortality rate was observed after day 5 of treatment. Mortality of engorged female ticks, inhibition of fecundity, and hatching of eggs were concentration dependent. The LC50 value of the extract against R. (B.) annulatus was 10.46%. The HPTLC profiling of the petroleum ether extract revealed the presence of at least seven polyvalent components. In the petroleum ether extract, nicotine was identified as one of the components up to a concentration of 5.4%. However, nicotine did not reveal any acaricidal activity up to 20000 ppm (2%). Coconut oil, used as diluent for dissolving the extract, did not reveal any acaricidal effects. The results are indicative of the involvement of synergistic or additive action of the bioactive components in the tick mortality and inhibition of the oviposition.
authors.affiliation: Department of Veterinary Pharmacology and Toxicology, College of Veterinary and Animal Sciences, Kerala Veterinary and Animal Sciences University, Pookode,Wayanad, Kerala 673 576, India.
authors.forename: T P Adarsh
authors.initials: TP
authors.lastname: Krishna
authors.affiliation: Department of Veterinary Pharmacology and Toxicology, College of Veterinary and Animal Sciences, Kerala Veterinary and Animal Sciences University, Pookode,Wayanad, Kerala 673 576, India.
authors.forename: T P Ajeesh
authors.initials: TP
authors.lastname: Krishna
authors.affiliation: Department of Veterinary Parasitology, College of Veterinary and Animal Sciences, Kerala Veterinary and Animal Sciences University, Pookode,Wayanad, Kerala 673 576, India.
authors.forename: N D
authors.initials: ND
authors.lastname: Chithra
authors.affiliation: Department of Veterinary Parasitology, College of Veterinary and Animal Sciences, Kerala Veterinary and Animal Sciences University, Pookode,Wayanad, Kerala 673 576, India.
authors.forename: P E
authors.initials: PE
authors.lastname: Deepa
authors.affiliation: Department of Veterinary Pharmacology and Toxicology, College of Veterinary and Animal Sciences, Kerala Veterinary and Animal Sciences University, Pookode,Wayanad, Kerala 673 576, India.
authors.forename: U
authors.initials: U
authors.lastname: Darsana
authors.affiliation: Department of Veterinary Pharmacology and Toxicology, College of Veterinary and Animal Sciences, Kerala Veterinary and Animal Sciences University, Pookode,Wayanad, Kerala 673 576, India.
authors.forename: K P
authors.initials: KP
authors.lastname: Sreelekha
authors.affiliation: Department of Veterinary Pharmacology and Toxicology, College of Veterinary and Animal Sciences, Kerala Veterinary and Animal Sciences University, Pookode,Wayanad, Kerala 673 576, India.
authors.forename: Sanis
authors.initials: S
authors.lastname: Juliet
authors.affiliation: Department of Veterinary Pharmacology and Toxicology, College of Veterinary and Animal Sciences, Kerala Veterinary and Animal Sciences University, Pookode,Wayanad, Kerala 673 576, India.
authors.forename: Suresh N
authors.initials: SN
authors.lastname: Nair
authors.affiliation: Department of Veterinary Parasitology, College of Veterinary and Animal Sciences, Kerala Veterinary and Animal Sciences University, Pookode,Wayanad, Kerala 673 576, India.
authors.forename: Reghu
authors.identifier: 0000-0001-9250-0552
authors.initials: R
authors.lastname: Ravindran
authors.affiliation: Department of Veterinary Parasitology, College of Veterinary and Animal Sciences, Kerala Veterinary and Animal Sciences University, Pookode,Wayanad, Kerala 673 576, India.
authors.forename: K G Ajith
authors.initials: KG
authors.lastname: Kumar
authors.affiliation: Entomology Laboratory, Division of Veterinary Parasitology, Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122, India.
authors.forename: Srikanta
authors.initials: S
authors.lastname: Ghosh
mesh_terms: D056810:Acaricides / Q000302:isolation & purification / Q000494:pharmacology*; D000473:Alkanes / Q000302:isolation & purification / Q000494:pharmacology*; D000818:Animals; D005260:Female; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D048494:Rhipicephalus / Q000187:drug effects*; D027844:Vitaceae
chemical_list: D056810:Acaricides; D000473:Alkanes; D010936:Plant Extracts; C004544:naphtha