title: Developmental exposure to purity-controlled polychlorinated biphenyl congeners (PCB74 and PCB95) in rats: effects on brainstem auditory evoked potentials and catalepsy.
abstract: Whereas the effects of dioxin-like polychlorinated biphenyls (DL-PCBs) are well described, less is known about non-dioxin-like PCBs (NDL-PCBs), including influences on the nervous system and related behavioral effects after developmental exposure. Following the examination of the highly purified NDL congeners PCB52 and PCB180, we report here the results of experiments with PCB74 and PCB95. Rat dams were orally exposed to equimolar doses of either congener (40μmol/kg bw - 11.68mg PCB74/kg bw or 13.06mg PCB95/kg bw) from gestational day (GD) 10 to postnatal day (PND) 7. Control dams were given the vehicle. Adult offspring were tested for cataleptic behavior after induction with haloperidol, a classical neuroleptic drug, and brainstem auditory evoked potentials (BAEPs), using clicks and tone pips of different frequencies for stimulation. Results revealed slight effects on latencies to movement onset in female offspring exposed to PCB74, whereas PCB74 males and offspring exposed to PCB95 were not affected. Pronounced changes were observed in BAEPs at low frequencies in PCB74 offspring, with elevated thresholds in both sexes. PCB95 increased thresholds in males, but not females. Small effects were detected on latency of the late wave IV in both sexes after developmental exposure to PCB74 or PCB95. Compared with the other NDL-PCB congeners tested, PCB74 caused the most pronounced effects on BAEPs.
authors.affiliation: Center of Toxicology, IPA - Institute for Prevention and Occupational Medicine, German Social Accident Insurance, Ruhr University of Bochum, Buerkle de la Camp Platz 1, 44789 Bochum, Germany. Electronic address: lilienthal@ipa.rub.de.
authors.forename: Hellmuth
authors.initials: H
authors.lastname: Lilienthal
authors.affiliation: Department of Environmental Health, THL - National Institute for Health and Welfare, 70701 Kuopio, Finland; Department of Environmental Science, University of Eastern Finland, 70211 Kuopio, Finland. Electronic address: merja.korkalainen@thl.fi.
authors.forename: Merja
authors.initials: M
authors.lastname: Korkalainen
authors.affiliation: Department of Chemistry, Umeå University, 901 87 Umeå, Sweden. Electronic address: patrik.andersson@chem.umu.se.
authors.forename: Patrik L
authors.initials: PL
authors.lastname: Andersson
authors.affiliation: Department of Environmental Health, THL - National Institute for Health and Welfare, 70701 Kuopio, Finland; Department of Environmental Science, University of Eastern Finland, 70211 Kuopio, Finland. Electronic address: matti.viluksela@thl.fi.
authors.forename: Matti
authors.initials: M
authors.lastname: Viluksela
mesh_terms: D000818:Animals; D001933:Brain Stem / Q000187:drug effects* / Q000502:physiology; D002375:Catalepsy / Q000139:chemically induced*; D005072:Evoked Potentials, Auditory / Q000187:drug effects*; D005260:Female; D006220:Haloperidol / Q000008:administration & dosage; D008297:Male; D011078:Polychlorinated Biphenyls / Q000633:toxicity*; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects; D051381:Rats; D020318:Rats, Long-Evans
chemical_list: C032904:2,2',3,5',6-pentachlorobiphenyl; D011078:Polychlorinated Biphenyls; D006220:Haloperidol; C541132:2,4,4',5-tetrachlorobiphenyl
keywords: Auditory development; Behavior; Catalepsy; Dopamine; Polychlorinated biphenyls; Rats