title: Differential expression of microRNAs in sexually immature and mature canine testes.
abstract: Mammalian testis exhibits spatiotemporal gene expression patterns that are essential for successful and continuous spermatogenesis. Although microRNAs (miRNAs) modify gene expression through translational repression and degradation of target messenger RNAs, the precise molecular mechanisms of these regulatory processes are unclear. We used canine miScript miRNA polymerase chain reaction (PCR) Array technology to elucidate the repertoire of canine testis miRNAs and compared their expression patterns between sexually immature (prepubertal) and mature (adult) dog testes. Eighty-four well-characterized canine miRNAs were customized in this study. The data were analyzed by RT(2) Profiler PCR Array Data Analysis (version 3.5). Results identified upregulation of 32 and considerable downregulation of 12 miRNAs in adult dog testis. In conclusion, the two developmental stages had significantly different miRNAs expression patterns. The finding provides fundamental information of miRNAs which may help to elucidate their role in spermatogenesis and male infertility in this species. To the best of our knowledge, this study is the first to offer comparative profile of the miRNA transcriptome in prepubertal and adult canine testes using miRNA PCR array approach.
authors.affiliation: Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Washington State University, Pullman, Washington, USA. Electronic address: vkasiman@vetmed.wsu.edu.
authors.forename: Vanmathy R
authors.initials: VR
authors.lastname: Kasimanickam
authors.affiliation: Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Washington State University, Pullman, Washington, USA.
authors.forename: Ramanathan K
authors.initials: RK
authors.lastname: Kasimanickam
mesh_terms: D000818:Animals; D004285:Dogs / Q000235:genetics*; D008297:Male; D035683:MicroRNAs / Q000378:metabolism*; D012741:Sexual Maturation / Q000235:genetics*; D013737:Testis / Q000378:metabolism*
chemical_list: D035683:MicroRNAs
keywords: Adult; Dog; MicroRNA expression; Prepubertal; Testis