title: Effects of long-term football training on the expression profile of genes involved in muscle oxidative metabolism.
abstract: We investigated whether long-term recreational football training affects the expression of health-related biochemical and molecular markers in healthy untrained subjects. Five untrained healthy men trained for 1 h 2.4 times/week for 12 weeks and 1.3 times/week for another 52 weeks. Blood samples and a muscle biopsy from the vastus lateralis were collected at T0 (pre intervention) and at T1 (post intervention). Gene expression was measured by RTqPCR on RNA extracted from muscle biopsies. The expression levels of the genes principally involved in energy metabolism (PPARγ, adiponectin, AMPKα1/α2, TFAM, NAMPT, PGC1α and SIRT1) were measured at T0 and T1. Up-regulation of PPARγ (p < 0.0005), AMPKα1 (p < 0.01), AMPKα2 (p < 0.0005) and adiponectin was observed at T1 vs T0. Increases were also found in the expression of TFAM (p < 0.001), NAMPT (p < 0.01), PGC1α (p < 0.01) and SIRT1 (p < 0.01), which are directly or indirectly involved in the glucose and lipid oxidative metabolism. Multiple linear regression analysis revealed that fat percentage was independently associated with NAMPT, PPARγ and adiponectin expression. In conclusion, long-term recreational football training could be a useful tool to improve the expression of muscle molecular biomarkers that are correlated to oxidative metabolism in healthy males.
authors.affiliation: Department of Movement Sciences and Wellness (DiSMEB), University Parthenope, Naples, Italy; CEINGE - Biotecnologie Avanzate, Naples, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Alfieri
authors.affiliation: CEINGE - Biotecnologie Avanzate, Naples, Italy.
authors.forename: D
authors.initials: D
authors.lastname: Martone
authors.affiliation: Copenhagen Centre for Team Sport and Health, Department of Nutrition, Exercise and Sports, University of Copenhagen, Copenhagen, Denmark.
authors.forename: M B
authors.initials: MB
authors.lastname: Randers
authors.affiliation: IRCCS SDN, Naples, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Labruna
authors.affiliation: Department of Movement Sciences and Wellness (DiSMEB), University Parthenope, Naples, Italy; CEINGE - Biotecnologie Avanzate, Naples, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Mancini
authors.affiliation: Copenhagen Centre for Team Sport and Health, Department of Nutrition, Exercise and Sports, University of Copenhagen, Copenhagen, Denmark.
authors.forename: J J
authors.initials: JJ
authors.lastname: Nielsen
authors.affiliation: Copenhagen Centre for Team Sport and Health, Department of Nutrition, Exercise and Sports, University of Copenhagen, Copenhagen, Denmark.
authors.forename: J
authors.initials: J
authors.lastname: Bangsbo
authors.affiliation: Copenhagen Centre for Team Sport and Health, Department of Nutrition, Exercise and Sports, University of Copenhagen, Copenhagen, Denmark; Sport and Health Sciences, College of Life and Environmental Sciences, University of Exeter, United Kingdom.
authors.forename: P
authors.initials: P
authors.lastname: Krustrup
authors.affiliation: Department of Movement Sciences and Wellness (DiSMEB), University Parthenope, Naples, Italy; IRCCS SDN, Naples, Italy. Electronic address: buono@uniparthenope.it.
authors.forename: P
authors.initials: P
authors.lastname: Buono
mesh_terms: D000222:Adaptation, Physiological; D052242:Adiponectin / Q000235:genetics; D000328:Adult; D015415:Biomarkers / Q000097:blood*; D001706:Biopsy; D004734:Energy Metabolism; D005080:Exercise Test; D005538:Football / Q000502:physiology*; D020869:Gene Expression Profiling / Q000379:methods*; D005786:Gene Expression Regulation; D064368:Healthy Volunteers; D006801:Humans; D008297:Male; D010084:Oxidation-Reduction; D047495:PPAR gamma / Q000235:genetics; D052097:Quadriceps Muscle / Q000378:metabolism* / Q000473:pathology
chemical_list: C499918:ADIPOQ protein, human; D052242:Adiponectin; D015415:Biomarkers; D047495:PPAR gamma
keywords: Football; LV-HIT; Messenger expression; Muscle biopsies; Recreational training