title: Genetic diversity of Dirofilaria spp. isolated from subcutaneous and ocular lesions of human patients in Ukraine.
abstract: This short communication describes the phylogenetic analysis of 48 Dirofilaria worms isolated from human patients in Ukraine. 102 cases were both of subcutaneous (47; 46.1%) and ocular (54; 52.9%) locations. Worms from 44 patients (15 subcutaneous and 29 ocular) were subjected to DNA extraction and amplification of a specific fragment of the 12S rRNA subunit, and sequences were used for phylogenetic analysis. Results showed that 13.8% of the ocular cases analyzed at molecular level were caused by Dirofilaria immitis. Very few cases of ocular human dirofilariosis due to D. immitis have been described in the literature to date, majority of them attributed to Dirofilaria repens. Our results show that ocular dirofilariosis cannot be excluded in areas of low endemicity for D. repens were D. immitis is also present.
authors.affiliation: Instituto de Recursos Naturales y Agrobiología de Salamanca, Agencia Estatal Consejo Superior de Investigaciones Científicas, Salamanca, Spain.
authors.forename: Alice
authors.initials: A
authors.lastname: Rossi
authors.affiliation: Instituto de Recursos Naturales y Agrobiología de Salamanca, Agencia Estatal Consejo Superior de Investigaciones Científicas, Salamanca, Spain.
authors.forename: Álvaro
authors.initials: Á
authors.lastname: Peix
authors.affiliation: Central Sanitary and Epidemiological Station of the Ukrainian Ministry of Health, Kiev, Ukraine.
authors.forename: Tamara
authors.initials: T
authors.lastname: Pavlikovskaya
authors.affiliation: Central Sanitary and Epidemiological Station of the Ukrainian Ministry of Health, Kiev, Ukraine.
authors.forename: Olga
authors.initials: O
authors.lastname: Sagach
authors.affiliation: Central Sanitary and Epidemiological Station of the Ukrainian Ministry of Health, Kiev, Ukraine.
authors.forename: Svetlana
authors.initials: S
authors.lastname: Nikolaenko
authors.affiliation: Central Sanitary and Epidemiological Station of the Ukrainian Ministry of Health, Kiev, Ukraine.
authors.forename: Nina
authors.initials: N
authors.lastname: Chizh
authors.affiliation: Department of Infectious Diseases, Rostov State Medical University, Rostov-na-Donu, Russia.
authors.forename: Vladimir
authors.initials: V
authors.lastname: Kartashev
authors.affiliation: Laboratory of Parasitology, Faculty of Pharmacy & IBSAL, University of Salamanca, Salamanca, Spain.
authors.forename: Fernando
authors.initials: F
authors.lastname: Simón
authors.affiliation: Instituto de Recursos Naturales y Agrobiología de Salamanca, Agencia Estatal Consejo Superior de Investigaciones Científicas, Salamanca, Spain. Electronic address: mmar.siles@irnasa.csic.es.
authors.forename: Mar
authors.initials: M
authors.lastname: Siles-Lucas
mesh_terms: D000818:Animals; D001483:Base Sequence; D018509:DNA, Helminth / Q000032:analysis; D004182:Dirofilaria / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D004184:Dirofilariasis / Q000469:parasitology*; D015822:Eye Infections, Parasitic / Q000469:parasitology*; D014644:Genetic Variation; D006801:Humans; D008969:Molecular Sequence Data; D010802:Phylogeny; D012335:RNA, Ribosomal / Q000032:analysis; D012876:Skin Diseases, Parasitic / Q000469:parasitology*; D014455:Ukraine
chemical_list: D018509:DNA, Helminth; D012335:RNA, Ribosomal; C075526:RNA, ribosomal, 12S
keywords: 12S RNA; Dirofilaria immitis; Dirofilaria repens; Ocular; Subcutaneous; Ukraine