title: Vegetable peptones increase production of type I collagen in human fibroblasts by inducing the RSK-CCAAT/enhancer binding protein-β phosphorylation pathway.
abstract: Skin aging appears to be principally attributed to a decrease in type I collagen level and the regeneration ability of dermal fibroblasts. We hypothesized that vegetable peptones promote cell proliferation and production of type I collagen in human dermal fibroblasts. Therefore, we investigated the effects of vegetable peptones on cell proliferation and type I collagen production and their possible mechanisms in human dermal fibroblasts. Vegetable peptones significantly promoted cell proliferation in a concentration-dependent manner. In addition, the human luciferase type I collagen α2 promoter and type I procollagen synthesis assays showed that the vegetable peptones induced type I procollagen production by activating the type I collagen α2 promoter. Moreover, the vegetable peptones activated p90 ribosomal s6 kinase, which was mediated by activating the Raf-p44/42 mitogen-activated protein kinase signaling pathway. Furthermore, the vegetable peptone-induced increase in cell proliferation and type I collagen production decreased upon treatment with the ERK inhibitor PD98059. Taken together, these findings suggest that increased proliferation of human dermal fibroblasts and enhanced production of type I collagen by vegetable peptones occur primarily by inducing the p90 ribosomal s6 kinase-CCAAT/enhancer binding protein β phosphorylation pathway, which is mediated by activating Raf-ERK signaling.
authors.affiliation: Biospectrum Life Science Institute, Seongnam City, 462-807 Gyeonggi-Do, Republic of Korea.
authors.forename: Eunsun
authors.initials: E
authors.lastname: Jung
authors.affiliation: Department of Genetic Engineering, Sungkyunkwan University, Suwon-Si, 440-746 Gyeonggi-Do, Republic of Korea.
authors.forename: Jae Youl
authors.initials: JY
authors.lastname: Cho
authors.affiliation: Biospectrum Life Science Institute, Seongnam City, 462-807 Gyeonggi-Do, Republic of Korea.
authors.forename: Deokhoon
authors.initials: D
authors.lastname: Park
authors.affiliation: Department of Physical Therapy, College of Health Science, Eulji University, Seongnam City, 461-713 Gyeonggi-Do, Republic of Korea.
authors.forename: Min Hee
authors.initials: MH
authors.lastname: Kim
authors.affiliation: Department of Senior Healthcare, BK21 plus program, Graduated school, Eulji University, Seongnam City, 461-713 Gyeonggi-Do, Republic of Korea.
authors.forename: Beomseok
authors.initials: B
authors.lastname: Park
authors.affiliation: Department of Life Science, Gachon University, San 65, Bokjeong-Dong, Sujeong-Gu, Seongnam-Si, 461-701 Gyeonggi-Do, Republic of Korea. Electronic address: leesaye@gachon.ac.kr.
authors.forename: Sang Yeol
authors.initials: SY
authors.lastname: Lee
authors.affiliation: Department of Dermatological Health Management, College of Health Science, Eulji University, Seongnam City, 461-713 Gyeonggi-Do, Republic of Korea; Department of Senior Healthcare, BK21 plus program, Graduated school, Eulji University, Seongnam City, 461-713 Gyeonggi-Do, Republic of Korea. Electronic address: jslee@eulji.ac.kr.
authors.forename: Jongsung
authors.initials: J
authors.lastname: Lee
mesh_terms: D022782:CCAAT-Enhancer-Binding Protein-beta / Q000378:metabolism; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D024042:Collagen Type I / Q000378:metabolism*; D005347:Fibroblasts / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D010461:Peptones / Q000494:pharmacology*; D010766:Phosphorylation / Q000187:drug effects; D011401:Promoter Regions, Genetic / Q000187:drug effects; D038744:Ribosomal Protein S6 Kinases, 90-kDa / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects*; D016896:Treatment Outcome; D014675:Vegetables / Q000737:chemistry*
chemical_list: D022782:CCAAT-Enhancer-Binding Protein-beta; D024042:Collagen Type I; D010461:Peptones; D038744:Ribosomal Protein S6 Kinases, 90-kDa
keywords: Cell proliferation; Human dermal fibroblasts; RSK pathway; Skin aging; Type I procollagen; Vegetable peptones