title: Prevalence of groups A and C rotavirus antibodies in infants with biliary atresia and cholestatic controls.
abstract: OBJECTIVE
  To analyze the prevalence of acute asymptomatic group A and C rotavirus (RV-A and RV-C) infection in neonates with cholestasis.
  
  
  METHODS
  Participants were infants <180 days of age with cholestasis (serum direct or conjugated bilirubin >20% of total and ≥2 mg/dL) enrolled in the Childhood Liver Disease Research and Education Network during RV season (December-May). Forty infants with biliary atresia (BA), age 62 ± 29 days (range, 4.7-13 weeks) and 38 infants with cholestasis, age 67 ± 44 days (range, 3-15.8 weeks) were enrolled.
  
  
  RESULTS
  At enrollment, RV-A IgM positivity rates did not differ between infants with BA (10%) vs those without (18%) (P = .349). RV-C IgM was positive in 0% of infants with BA vs 3% in those without BA (P = .49). RV-A IgG was lower in infants with BA: 51 ± 39 vs 56 ± 44 enzyme-linked immunoassay unit, P = .045 but this difference may lack biological relevance as maternal RV-A IgG titers were similar between groups. Infant RV-A IgM titers at 2-6 months follow-up increased markedly vs at presentation in both infants with BA (50 ± 30 vs 9 ± 9) and those without (43 ± 18 vs 16 ± 20 enzyme-linked immunoassay unit) (P < .0001), without differences between groups.
  
  
  CONCLUSIONS
  RV-A infection in the first 6 months of life is common in infants with cholestasis of any cause. RV-A could have different pathogenetic effects by initiating different hepatic immune responses in infants with vs without BA or could lack pathogenetic significance.
authors.affiliation: Department of Pediatrics, Johns Hopkins Medical Institutions, Baltimore, MD.
authors.forename: Maria Grazia
authors.initials: MG
authors.lastname: Clemente
authors.affiliation: Laboratory of Infectious Diseases, National Institutes of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD.
authors.forename: John T
authors.initials: JT
authors.lastname: Patton
authors.affiliation: Stanley Division of Developmental Neurovirology, Johns Hopkins University School of Medicine, Baltimore, MD.
authors.forename: Robert
authors.initials: R
authors.lastname: Yolken
authors.affiliation: Department of Pediatrics, Feinberg School of Medicine of Northwestern University, Chicago, IL.
authors.forename: Peter F
authors.initials: PF
authors.lastname: Whitington
authors.affiliation: Division of Viral Diseases, Centers for Disease Control and Prevention, Atlanta, GA.
authors.forename: Umesh D
authors.initials: UD
authors.lastname: Parashar
authors.affiliation: Division of Viral Diseases, Centers for Disease Control and Prevention, Atlanta, GA.
authors.forename: Baoming
authors.initials: B
authors.lastname: Jiang
authors.affiliation: Department of Biostatistics, University of Michigan School of Public Health, Ann Arbor, MI.
authors.forename: Trivellore
authors.initials: T
authors.lastname: Raghunathan
authors.affiliation: Department of Pediatrics, Johns Hopkins Medical Institutions, Baltimore, MD. Electronic address: kschwarz@jhmi.edu.
authors.forename: Kathleen B
authors.initials: KB
authors.lastname: Schwarz
mesh_terms: D000914:Antibodies, Viral / Q000097:blood*; D001656:Biliary Atresia / Q000276:immunology* / Q000821:virology; D016022:Case-Control Studies; D002779:Cholestasis / Q000276:immunology* / Q000821:virology; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D007075:Immunoglobulin M / Q000097:blood; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D015995:Prevalence; D012189:Retrospective Studies; D012401:Rotavirus / Q000276:immunology*; D012400:Rotavirus Infections / Q000276:immunology* / Q000821:virology; D016036:Seroepidemiologic Studies
chemical_list: D000914:Antibodies, Viral; D007074:Immunoglobulin G; D007075:Immunoglobulin M