title: Augmentation of diabetic wound healing and enhancement of collagen content using nanofibrous glucophage-loaded collagen/PLGA scaffold membranes.
abstract: This work developed nanofibrous drug-loaded collagen/poly-D-L-lactide-glycolide (PLGA) scaffold membranes that provided the sustained release of glucophage for the wounds associated with diabetes. PLGA, glucophage, and collagen were firstly dissolved in 1,1,1,3,3,3-hexafluoro-2-propanol and were spun into nanofibrous membranes by electrospinning. High-performance liquid chromatography assay was used to characterize the in vivo and in vitro release rates of the pharmaceuticals from the membranes. High concentrations of glucophage were released for over three weeks from the nanofibrous membranes. The nanofibrous glucophage-loaded collagen/PLGA membranes were more hydrophilic than collagen/PLGA membranes and exhibited a greater water-containing capacity. The glucophage-loaded collagen/PLGA membranes markedly promoted the healing of diabetic wounds. Moreover, the collagen content of diabetic rats using drug-eluting membranes was higher than that of the control rats, because of the down-regulation of matrix metalloproteinase 9. The experimental results herein suggest that the nanofibrous glucophage-loaded collagen/PLGA membranes had effect for increasing collagen content in treating diabetic wounds and very effective promoters of the healing of such wounds in the early stages.
authors.affiliation: Division of Cardiology, Department of Internal Medicine, Chang Gung Memorial Hospital-Linkou, Chang Gung University College of Medicine, Tao-Yuan, Taiwan; Department of Mechanical Engineering, Chang Gung University, Tao-Yuan, Taiwan.
authors.forename: Cheng-Hung
authors.initials: CH
authors.lastname: Lee
authors.affiliation: Division of Cardiology, Department of Internal Medicine, Chang Gung Memorial Hospital-Linkou, Chang Gung University College of Medicine, Tao-Yuan, Taiwan.
authors.forename: Shang-Hung
authors.initials: SH
authors.lastname: Chang
authors.affiliation: Division of Cardiology, Department of Internal Medicine, Chang Gung Memorial Hospital-Linkou, Chang Gung University College of Medicine, Tao-Yuan, Taiwan.
authors.forename: Wei-Jan
authors.initials: WJ
authors.lastname: Chen
authors.affiliation: Division of Cardiology, Department of Internal Medicine, Chang Gung Memorial Hospital-Linkou, Chang Gung University College of Medicine, Tao-Yuan, Taiwan.
authors.forename: Kuo-Chun
authors.initials: KC
authors.lastname: Hung
authors.affiliation: Department of Mechanical Engineering, Chang Gung University, Tao-Yuan, Taiwan.
authors.forename: Yu-Huang
authors.initials: YH
authors.lastname: Lin
authors.affiliation: Department of Mechanical Engineering, Chang Gung University, Tao-Yuan, Taiwan. Electronic address: shihjung@mail.cgu.edu.tw.
authors.forename: Shih-Jung
authors.initials: SJ
authors.lastname: Liu
authors.affiliation: Division of Cardiology, Department of Internal Medicine, Chang Gung Memorial Hospital-Linkou, Chang Gung University College of Medicine, Tao-Yuan, Taiwan.
authors.forename: Ming-Jer
authors.initials: MJ
authors.lastname: Hsieh
authors.affiliation: Graduate Institute of Clinical Medical Sciences, Chang Gung University, Kwei-Shan, Tao-Yuan, Taiwan.
authors.forename: Jong-Hwei S
authors.initials: JH
authors.lastname: Pang
authors.affiliation: Division of Endocrinology and Metabolism, Department of Internal Medicine, Chang Gung University and Chang Gung Memorial Hospital, Tao-Yuan, Taiwan.
authors.forename: Jyuhn-Huarng
authors.initials: JH
authors.lastname: Juang
mesh_terms: D000818:Animals; D003094:Collagen / Q000737:chemistry / Q000378:metabolism*; D003921:Diabetes Mellitus, Experimental; D004333:Drug Administration Routes; D007004:Hypoglycemic Agents / Q000737:chemistry / Q000494:pharmacology; D019344:Lactic Acid / Q000737:chemistry* / Q000494:pharmacology; D008297:Male; D008567:Membranes, Artificial; D008687:Metformin / Q000737:chemistry / Q000494:pharmacology; D057139:Nanofibers / Q000737:chemistry*; D011100:Polyglycolic Acid / Q000737:chemistry* / Q000494:pharmacology; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D051381:Rats; D054457:Tissue Scaffolds; D014945:Wound Healing / Q000187:drug effects
chemical_list: D007004:Hypoglycemic Agents; D008567:Membranes, Artificial; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D003094:Collagen; D008687:Metformin
keywords: Diabetic wound repair; Electrospinning; Nanofibrous glucophage-loaded collagen/PLGA membranes; Release characteristics