title: Sensitive detection of C-reactive protein using optical fiber Bragg gratings.
abstract: An accurate and highly sensitive sensor platform has been demonstrated for the detection of C-reactive protein (CRP) using optical fiber Bragg gratings (FBGs). The CRP detection has been carried out by monitoring the shift in Bragg wavelength (ΔλB) of an etched FBG (eFBG) coated with an anti-CRP antibody (aCRP)-graphene oxide (GO) complex. The complex is characterized by Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy and atomic force microscopy. A limit of detection of 0.01mg/L has been achieved with a linear range of detection from 0.01mg/L to 100mg/L which includes clinical range of CRP. The eFBG sensor coated with only aCRP (without GO) show much less sensitivity than that of aCRP-GO complex coated eFBG. The eFBG sensors show high specificity to CRP even in the presence of other interfering factors such as urea, creatinine and glucose. The affinity constant of ∼1.1×10(10)M(-1) has been extracted from the data of normalized shift (ΔλB/λB) as a function of CRP concentration.
authors.affiliation: Department of Instrumentation and Applied Physics, Indian Institute of Science, Bangalore 560012, India.
authors.forename: S
authors.initials: S
authors.lastname: Sridevi
authors.affiliation: Department of Physics, Indian Institute of Science, Bangalore 560012, India.
authors.forename: K S
authors.initials: KS
authors.lastname: Vasu
authors.affiliation: Department of Instrumentation and Applied Physics, Indian Institute of Science, Bangalore 560012, India; Robert Bosch Centre for Cyber Physical Systems, Indian Institute of Science, Bangalore 560012, India.
authors.forename: S
authors.initials: S
authors.lastname: Asokan
authors.affiliation: Department of Physics, Indian Institute of Science, Bangalore 560012, India. Electronic address: asood1951@gmail.com.
authors.forename: A K
authors.initials: AK
authors.lastname: Sood
mesh_terms: D055910:Antibodies, Immobilized / Q000737:chemistry; D015374:Biosensing Techniques / Q000379:methods*; D002097:C-Reactive Protein / Q000032:analysis*; D005336:Fiber Optic Technology / Q000379:methods*; D006108:Graphite / Q000737:chemistry; D006801:Humans; D057230:Limit of Detection; D055100:Optical Fibers; D010087:Oxides / Q000737:chemistry
chemical_list: D055910:Antibodies, Immobilized; D010087:Oxides; D006108:Graphite; D002097:C-Reactive Protein
keywords: Bragg wavelength; C-reactive protein; Fiber Bragg gratings; Graphene oxide