title: Rapid method to screen and sort lipid accumulating microalgae.
abstract: The present work established an efficient staining method for fluorescence activated cell sorting (FACS) with Chlorococcum littorale maintaining cellular viability. The method was designed to detect high-lipid cells and to guarantee cellular viability. BODIPY505/515 (BP) was more suitable to FACS when compared to Nile red. The optimum concentrations were 0.4 μg ml(-1) of BP, 0.1% DMSO or 0.35% ethanol. Both ethanol and DMSO were equally efficient and assured cellular viability after the staining and sorting. Here a method is presented to rapidly screen and sort lipid rich cells of C. littorale with FACS, which can be used to produce new inoculum with increased cellular lipid content.
authors.affiliation: Wageningen University, Bioprocess Engineering, AlgaePARC, P.O. Box 8128, 6700 EV Wageningen, Netherlands; Wageningen University and Research Center, Food and Biobased Research, AlgaePARC, Bornsesteeg 10, Building 112, 6721NG Bennekom, Netherlands. Electronic address: iago.dominguezteles@wur.nl.
authors.forename: Iago Teles Dominguez
authors.initials: ITD
authors.lastname: Cabanelas
authors.affiliation: Wageningen University, Bioprocess Engineering, AlgaePARC, P.O. Box 8128, 6700 EV Wageningen, Netherlands.
authors.forename: Mathijs van der
authors.initials: MV
authors.lastname: Zwart
authors.affiliation: Wageningen University and Research Center, Food and Biobased Research, AlgaePARC, Bornsesteeg 10, Building 112, 6721NG Bennekom, Netherlands.
authors.forename: Dorinde M M
authors.initials: DMM
authors.lastname: Kleinegris
authors.affiliation: Wageningen University and Research Center, Food and Biobased Research, AlgaePARC, Bornsesteeg 10, Building 112, 6721NG Bennekom, Netherlands.
authors.forename: Maria J
authors.initials: MJ
authors.lastname: Barbosa
authors.affiliation: Wageningen University, Bioprocess Engineering, AlgaePARC, P.O. Box 8128, 6700 EV Wageningen, Netherlands.
authors.forename: René H
authors.initials: RH
authors.lastname: Wijffels
mesh_terms: D001709:Biotechnology / Q000379:methods*; D001896:Boron Compounds / Q000378:metabolism; D002469:Cell Separation; D002470:Cell Survival; D008055:Lipids / Q000096:biosynthesis*; D058086:Microalgae / Q000302:isolation & purification*; D010078:Oxazines / Q000378:metabolism
chemical_list: C095489:4,4-difluoro-4-bora-3a,4a-diaza-s-indacene; D001896:Boron Compounds; D008055:Lipids; D010078:Oxazines; C044808:nile red
keywords: BODIPY(505/515); Chlorococcum littorale; FACS; Lipids; Microalgae