title: Development of nitrogen-blowing salt-induced solidified floating organic droplet microextraction for determination of phenolic oestrogens in milk samples.
abstract: In solidified floating organic droplet microextraction, the solidified organic phase formed a concave meniscus on the water surface, and the solidified layer was easily broken into pieces when using a medical spoon to collect it, resulting in a slow and difficult whole collection. A novel method, nitrogen-blowing salt-induced solidified floating organic droplet microextraction, was developed for pretreatment of the milk samples. After the procedures, the down layer salt and aqueous phase was discarded by opening the bottom cap. The remaining solidified solvent was easily and wholly collected by a medical spoon, resulting in a high extraction efficiency. The similarly high deprotein rate (91-96%) was obtained between using acetonitrile and without using organic solvent as precipitating agent. Under optimized conditions, the limits of detection were 0.34-0.87 μg/L, the linear range was 1-500 μg/L, the extraction recoveries were 79.2-105.2%, and the enrichment factors were 105.8-169.3 for five phenolic oestrogens.
authors.affiliation: Department of Environmental Sciences, Wenzhou Medical University, Wenzhou 325035, China.
authors.forename: Yongkui
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Environmental Sciences, Wenzhou Medical University, Wenzhou 325035, China.
authors.forename: Mei
authors.initials: M
authors.lastname: Wang
authors.affiliation: College of Life Sciences, Wenzhou Medical University, Wenzhou 325035, China.
authors.forename: Huili
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of Environmental Sciences, Wenzhou Medical University, Wenzhou 325035, China. Electronic address: wangwenwei@wzmc.edu.cn.
authors.forename: Wenwei
authors.initials: W
authors.lastname: Wang
authors.affiliation: College of Life Sciences, Wenzhou Medical University, Wenzhou 325035, China.
authors.forename: Jia
authors.initials: J
authors.lastname: Wu
authors.affiliation: Department of Environmental Sciences, Wenzhou Medical University, Wenzhou 325035, China. Electronic address: zjuwxd@163.com.
authors.forename: Xuedong
authors.initials: X
authors.lastname: Wang
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004967:Estrogens / Q000737:chemistry*; D005504:Food Analysis; D006863:Hydrogen-Ion Concentration; D057230:Limit of Detection; D008892:Milk / Q000737:chemistry*; D009584:Nitrogen / Q000737:chemistry*; D010636:Phenols / Q000737:chemistry*; D012492:Salts / Q000737:chemistry*; D012965:Sodium Chloride / Q000737:chemistry; D012997:Solvents / Q000737:chemistry; D014867:Water / Q000737:chemistry
chemical_list: D004967:Estrogens; D010636:Phenols; D012492:Salts; D012997:Solvents; D014867:Water; D012965:Sodium Chloride; D009584:Nitrogen
keywords: HDPE tube; Milk sample; NS-SFODM method; Non-solvent deprotein; Phenolic oestrogens; Whole collection