title: Rapid screening of mycotoxins in liquid milk and milk powder by automated size-exclusion SPE-UPLC-MS/MS and quantification of matrix effects over the whole chromatographic run.
abstract: An automated, size-exclusion solid phase extraction (SPE)-UPLC-MS/MS protocol without pre-treatment of samples was developed to screen for four mycotoxins (OTA, ZEN, AFB1, and AFM1) in liquid milk and milk powder. Firstly, a mixed macropore-silica gel cartridge was established as a size-exclusion SPE column. The proposed methodology could be a candidate in green analytical chemistry because it saves on manpower and organic solvent. Permanent post-column infusion of mycotoxin standards was used to quantify matrix effects throughout the chromatographic run. Matrix-matched calibration could effectively compensate for matrix effects, which may be caused by liquid milk or milk powder matrix. Recovery of the four mycotoxins in fortified liquid milk was in the range 89-120% and RSD 2-9%. The LOD for the four mycotoxins in liquid milk and milk powder were 0.05-2 ng L(-1) and 0.25-10 ng kg(-1), respectively. The LOQ for the four mycotoxins in liquid milk and milk powder were 0.1-5 ng L(-1) and 0.5-25 ng kg(-1), respectively.
authors.affiliation: Oil Crops Research Institute of The Chinese Academy of Agricultural Sciences, Wuhan, PR China; Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Wuhan, PR China; Laboratory of Risk Assessment for Oilseeds Products (Wuhan), Ministry of Agriculture, PR China.
authors.forename: Xiupin
authors.initials: X
authors.lastname: Wang
authors.affiliation: Oil Crops Research Institute of The Chinese Academy of Agricultural Sciences, Wuhan, PR China; Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Wuhan, PR China; Key Laboratory of Detection for Mycotoxins, Ministry of Agriculture, Wuhan, PR China; Quality Inspection and Center for Oilseeds Productions, Ministry of Agriculture, Wuhan, PR China. Electronic address: peiwuli@oilcrops.cn.
authors.forename: Peiwu
authors.initials: P
authors.lastname: Li
mesh_terms: D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005506:Food Contamination / Q000032:analysis*; D008892:Milk / Q000737:chemistry*; D009183:Mycotoxins / Q000737:chemistry* / Q000302:isolation & purification*; D052616:Solid Phase Extraction / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D009183:Mycotoxins
keywords: Automated exclusion SPE; Liquid milk; Matrix effects; Milk powder; Permanent postcolumn infusion (PCI); UPLC–MS/MS