title: Dynamic expression of tgf-β2, tgf-β3 and inhibin βA during muscle growth resumption and satellite cell differentiation in rainbow trout (Oncorhynchus mykiss).
abstract: Members of the TGF-β superfamily are involved in numerous cell functions; however, except for myostatin, their roles in the regulation of muscle growth in fish are completely unknown. We measured tgf-β1, tgf-β2, tgf-β3, inhibin βA (inh) and follistatin (fst) gene expression during muscle growth recovery following a fasting period. We observed that tgf-β1a and tgf-β2 expression were quickly down-regulated after refeeding and that tgf-β3 reached its highest level of expression 7days post-refeeding, mirroring myogenin expression. Inh βA1 mRNA levels decreased sharply after refeeding, in contrast to fst b2 expression, which peaked at day 2. No significant modification of expression was observed for tgf-β1a, tgf-β1b, tgf-β1c and tgf-β6 during refeeding. In vitro, tgf-β2 and inh βA1 expression decreased during the differentiation of satellite cells, whereas tgf-β3 expression increased following the same pattern as myogenin. Surprisingly, fst b1 and fst b2 expression decreased during differentiation, whereas no variation was observed in fst a1 and fst a2 expression levels. In vitro analyses also indicated that IGF1 treatment up-regulated tgf-β3, inh βA1 and myogenin expression, and that MSTN treatment increased fst b1 and fst b2 expression. In conclusion, we showed that the expression of tgf-β2, tgf-β3 and inh βA1 is dynamically regulated during muscle growth resumption and satellite cell differentiation, strongly suggesting that these genes have a role in the regulation of muscle growth.
authors.affiliation: Federal University of Rio Grande do Sul, Faculty of Agricultural Sciences, Research Group AQUAM, Aquaculture Sector, Department of Animal Science, Avenue Bento Gonçalves 7712, Agronomia, Porto Alegre 91540-000, RS, Brazil.
authors.forename: Fernanda
authors.initials: F
authors.lastname: de Mello
authors.affiliation: Federal University of Rio Grande do Sul, Faculty of Agricultural Sciences, Research Group AQUAM, Aquaculture Sector, Department of Animal Science, Avenue Bento Gonçalves 7712, Agronomia, Porto Alegre 91540-000, RS, Brazil.
authors.forename: Danilo Pedro
authors.initials: DP
authors.lastname: Streit
authors.affiliation: INRA, UR1037 Laboratory of Fish Physiology and Genomic, Growth and Flesh Quality Group, campus de Beaulieu, 35000 Rennes, France.
authors.forename: Nathalie
authors.initials: N
authors.lastname: Sabin
authors.affiliation: INRA, UR1037 Laboratory of Fish Physiology and Genomic, Growth and Flesh Quality Group, campus de Beaulieu, 35000 Rennes, France. Electronic address: Jean-Charles.Gabillard@rennes.inra.fr.
authors.forename: Jean-Charles
authors.initials: JC
authors.lastname: Gabillard
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects / Q000235:genetics*; D002478:Cells, Cultured; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D013006:Growth Hormone / Q000494:pharmacology; D028322:Inhibin-beta Subunits / Q000235:genetics* / Q000378:metabolism; D007334:Insulin-Like Growth Factor I / Q000494:pharmacology; D024510:Muscle Development / Q000235:genetics*; D009132:Muscles / Q000187:drug effects / Q000502:physiology; D055435:Myostatin / Q000494:pharmacology; D017686:Oncorhynchus mykiss / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D032496:Satellite Cells, Skeletal Muscle / Q000187:drug effects / Q000502:physiology*; D053781:Transforming Growth Factor beta2 / Q000235:genetics* / Q000378:metabolism; D053782:Transforming Growth Factor beta3 / Q000235:genetics* / Q000378:metabolism
chemical_list: D055435:Myostatin; D053781:Transforming Growth Factor beta2; D053782:Transforming Growth Factor beta3; C103008:inhibin beta A subunit; D007334:Insulin-Like Growth Factor I; D013006:Growth Hormone; D028322:Inhibin-beta Subunits
keywords: GH; IGF1; Myostatin; Refeeding; Satellite cells; Starvation