title: Overexpression of microRNA biogenesis machinery: Drosha, DGCR8 and Dicer in multiple sclerosis patients.
abstract: We aimed to evaluate the expression of the major components of microRNA biogenesis machinery including Drosha, Dicer and DiGeorge syndrome critical region gene 8 (DGCR8) in multiple sclerosis (MS) patients. The expression levels of these components in relapsing remitting multiple sclerosis (RRMS) patients were significantly up-regulated in comparison to healthy controls. DGCR8 was up-regulated 4.9 times in RRMS patients versus healthy controls, and Drosha was up-regulated 3.58 times. Additionally, the expression level of Dicer was 2.11 times higher in RRMS patients than the healthy controls. In conclusion, our results suggest that overexpression of Drosha, Dicer and DGCR8 may contribute to the pathogenesis of MS. Further investigation may introduce microRNA biogenesis machinery as MS markers and therapeutic targets.
authors.affiliation: Department of Biochemistry, School of Medicine, Ardabil University of Medical Science, Ardabil, Iran; Department of Cell & Molecular Biology, School of Biology, College of Science, University of Tehran, Tehran, Iran.
authors.forename: Naser
authors.initials: N
authors.lastname: Jafari
authors.affiliation: Department of Biochemistry, School of Medicine, Ardabil University of Medical Science, Ardabil, Iran.
authors.forename: Hassan
authors.initials: H
authors.lastname: Shaghaghi
authors.affiliation: Department of Biochemistry, School of Medicine, Ardabil University of Medical Science, Ardabil, Iran.
authors.forename: Davood
authors.initials: D
authors.lastname: Mahmoodi
authors.affiliation: Department of Biochemistry, School of Medicine, Ardabil University of Medical Science, Ardabil, Iran.
authors.forename: Zohreh
authors.initials: Z
authors.lastname: Shirzad
authors.affiliation: Department of Biochemistry, School of Medicine, Ardabil University of Medical Science, Ardabil, Iran.
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Alibeiki
authors.affiliation: Department of Pharmacology, School of Pharmacy, Ardabil University of Medical Science, Ardabil, Iran.
authors.forename: Shahab
authors.initials: S
authors.lastname: Bohlooli
authors.affiliation: Department of Microbiology, School of Medicine, Ardabil University of Medical Science, Daneshgah Street, Ardabil 55136, Iran. Electronic address: hadi.peeridogaheh@arums.ac.ir.
authors.forename: Hadi Peeri
authors.initials: HP
authors.lastname: Dogaheh
mesh_terms: D000328:Adult; D053487:DEAD-box RNA Helicases / Q000096:biosynthesis* / Q000235:genetics*; D004247:DNA / Q000096:biosynthesis / Q000235:genetics; D004185:Disability Evaluation; D005260:Female; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000096:biosynthesis* / Q000235:genetics*; D009103:Multiple Sclerosis / Q000235:genetics*; D020529:Multiple Sclerosis, Relapsing-Remitting / Q000235:genetics; D016133:Polymerase Chain Reaction; D016601:RNA-Binding Proteins / Q000096:biosynthesis* / Q000235:genetics*; D043244:Ribonuclease III / Q000096:biosynthesis* / Q000235:genetics*; D015854:Up-Regulation
chemical_list: C474043:DGCR8 protein, human; D035683:MicroRNAs; D016601:RNA-Binding Proteins; D004247:DNA; C408087:DICER1 protein, human; C517918:DROSHA protein, human; D043244:Ribonuclease III; D053487:DEAD-box RNA Helicases
keywords: DGCR8; Dicer; Drosha; Gene expression; MicroRNA machinery; Multiple sclerosis