title: Kisspeptin regulates tuberoinfundibular dopaminergic neurones and prolactin secretion in an oestradiol-dependent manner in male and female rats.
abstract: Prolactin (PRL) secretion is inhibited by hypothalamic dopamine. Kisspeptin controls luteinising hormone (LH) secretion and is also involved in PRL regulation. We further investigated the effect of kisspeptin-10 (Kp-10) on the activity of tuberoinfundibular dopaminergic (TIDA) neurones and the role of oestradiol (E2 ) in this mechanism. Female and male rats were injected with i.c.v. Kp-10 and evaluated for PRL release and the activity of dopamine terminals in the median eminence (ME) and neurointermediate lobe of the pituitary (NIL). Kp-10 at the doses of 0.6 and 3 nmol increased plasma PRL and decreased 4-dihydroxyphenylacetic acid (DOPAC) levels in the ME and NIL of ovariectomised (OVX), E2 -treated rats but had no effect in OVX. In gonad-intact males, 3 nmol Kp-10 increased PRL secretion and decreased DOPAC levels in the ME but not in the NIL. Castrated males treated with either testosterone or E2 also displayed increased PRL secretion and reduced ME DOPAC in response to Kp-10, whereas castrated rats receiving oil or dihydrotestosterone were unresponsive. By contrast, the LH response to Kp-10 was not E2 -dependent in either females or males. Additionally, immunohistochemical double-labelling demonstrated that TIDA neurones of male rats contain oestrogen receptor (ER)-α, with a higher proportion of neurones expressing ERα than in dioestrous females. The dopaminergic neurones of periventricular hypothalamic nucleus displayed much lower ERα expression. Thus, TIDA neurones express ERα in male and female rats, and kisspeptin increases PRL secretion through inhibition of TIDA neurones in an E2 -dependent manner in both sexes. These findings provide new evidence about the role of kisspeptin in the regulation of dopamine and PRL.
authors.affiliation: Departamento de Fisiologia, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, Brazil.
authors.forename: A B
authors.initials: AB
authors.lastname: Ribeiro
authors.forename: C M
authors.initials: CM
authors.lastname: Leite
authors.forename: B
authors.initials: B
authors.lastname: Kalil
authors.forename: C R
authors.initials: CR
authors.lastname: Franci
authors.forename: J A
authors.initials: JA
authors.lastname: Anselmo-Franci
authors.forename: R E
authors.initials: RE
authors.lastname: Szawka
mesh_terms: D000818:Animals; D001111:Arcuate Nucleus of Hypothalamus / Q000378:metabolism*; D059290:Dopaminergic Neurons / Q000378:metabolism*; D004958:Estradiol / Q000378:metabolism*; D005260:Female; D059648:Kisspeptins / Q000502:physiology*; D007986:Luteinizing Hormone / Q000378:metabolism; D008297:Male; D011388:Prolactin / Q000378:metabolism*; D051381:Rats
chemical_list: C491406:Kiss1 protein, rat; D059648:Kisspeptins; D004958:Estradiol; D011388:Prolactin; D007986:Luteinizing Hormone
keywords: dopamine; kisspeptin; luteinising hormone; oestrogen; prolactin; serotonin