title: Development of a multiplex flow-through immunoaffinity chromatography test for the on-site screening of 14 sulfonamide and 13 quinolone residues in milk.
abstract: In this paper, a rapid and sensitive multiplex flow-through immunoaffinity chromatography test (FTIACT) was developed for the on-site screening of 14 sulfonamide and 13 quinolone residues in milk. The developed FTIACT method combines the purification, preconcentration and immunochemical detection of multiple antibiotics on the sepharose gel test layers. The use of liposome-encapsulated quantum dots (LQDs) with the FTIACT method exhibited the best results, with limits of detection (LODs) of 1 and 0.5ng/mL for the sulfonamides (SAs) and quinolones (QNs), respectively, through qualitative analysis (visual detection by the naked eye). In order to achieve low detection limit, the color intensity of the images were converted into relative optical density values to enable a quantitative evaluation. Quantitative analysis of the samples enabled the detection of SAs (0.13ng/mL) and QNs (0.062ng/mL) in spiked milk samples. The FTIACT described in this work shows promise as a multiplex immunoassay for the qualitative and quantitative screening of multiple chemical residues in milk.
authors.affiliation: Department of Pharmacology and Toxicology, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China; The Engineering Lab of Synthetic Biology and the Key Lab of Biomedical Engineering, School of Medicine, Shenzhen University, Shenzhen 518060, China.
authors.forename: Wenxiao
authors.initials: W
authors.lastname: Jiang
authors.affiliation: Laboratory of Food Analysis, Faculty of Pharmaceutical Sciences, Ghent University, Harelbekestraat 72, Ghent 9000, Belgium.
authors.forename: Natalia V
authors.initials: NV
authors.lastname: Beloglazova
authors.affiliation: Department of Pharmacology and Toxicology, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China.
authors.forename: Zhanhui
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Department of Pharmacology and Toxicology, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China.
authors.forename: Haiyang
authors.initials: H
authors.lastname: Jiang
authors.affiliation: Department of Pharmacology and Toxicology, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Wen
authors.affiliation: Laboratory of Food Analysis, Faculty of Pharmaceutical Sciences, Ghent University, Harelbekestraat 72, Ghent 9000, Belgium.
authors.forename: Sarah
authors.initials: S
authors.lastname: de Saeger
authors.affiliation: Key Laboratory of Food Safety Risk Assessment, Ministry of Health, China National Center For Food Safety Risk Assessment, Beijing 100021, China.
authors.forename: Pengjie
authors.initials: P
authors.lastname: Luo
authors.affiliation: Key Laboratory of Food Safety Risk Assessment, Ministry of Health, China National Center For Food Safety Risk Assessment, Beijing 100021, China.
authors.forename: Yongning
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Department of Pharmacology and Toxicology, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China. Electronic address: sjz@cau.edu.cn.
authors.forename: Jianzhong
authors.initials: J
authors.lastname: Shen
mesh_terms: D000818:Animals; D002846:Chromatography, Affinity / Q000295:instrumentation / Q000379:methods*; D005506:Food Contamination / Q000032:analysis; D057230:Limit of Detection; D008892:Milk / Q000737:chemistry*; D015363:Quinolones / Q000032:analysis*; D013449:Sulfonamides / Q000032:analysis*
chemical_list: D015363:Quinolones; D013449:Sulfonamides
keywords: Food safety; Immunoassay; Milk; Quinolones; Sulfonamides; Veterinary drug residue