title: Apoptotic primary normal human dermal fibroblasts for in vitro models of fibrosis.
abstract: Recent studies show that apoptosis affects surrounding tissue, playing a role in diseases such as fibrosis, a significant global disease burden. Elucidating the mechanisms by which the different apoptotic cells present during fibrotic wound healing affect their environment would enable development of new therapies. We describe here a simple, rapid, and cost-effective method for inducing apoptosis of primary normal human dermal fibroblasts without affecting the overall cell viability of the population. Such population could be used for in vitro models of fibrotic wound healing in co-culture with other cells involved in this process to study events such as apoptosis-induced proliferation.
authors.affiliation: RAFT Institute of Plastic Surgery, Mount Vernon Hospital, Northwood HA6 2RN, UK. Electronic address: garciae@raft.ac.uk.
authors.forename: Elena
authors.initials: E
authors.lastname: García-Gareta
authors.affiliation: RAFT Institute of Plastic Surgery, Mount Vernon Hospital, Northwood HA6 2RN, UK.
authors.forename: Nivedita
authors.initials: N
authors.lastname: Ravindran
authors.affiliation: RAFT Institute of Plastic Surgery, Mount Vernon Hospital, Northwood HA6 2RN, UK.
authors.forename: Julian F
authors.initials: JF
authors.lastname: Dye
mesh_terms: D017209:Apoptosis; D049109:Cell Proliferation; D003362:Cost-Benefit Analysis; D003584:Cytological Techniques / Q000191:economics / Q000379:methods*; D005347:Fibroblasts / Q000166:cytology* / Q000473:pathology; D005355:Fibrosis; D006801:Humans; D012867:Skin / Q000166:cytology*; D013997:Time Factors
keywords: Apoptosis; Cell viability; Ethanol; Fibrosis; Primary normal human dermal fibroblasts