title: Unstable simple volatiles and gas chromatography-tandem mass spectrometry analysis of essential oil from the roots bark of Oplopanax horridus extracted by supercritical fluid extraction.
abstract: Volatile oil from the root bark of Oplopanax horridus is regarded to be responsible for the clinical uses of the title plant as a respiratory stimulant and expectorant. Therefore, a supercritical fluid extraction method was first employed to extract the volatile oil from the roots bark of O. horridus, which was subsequently analyzed by GC/MS. Forty-eight volatile compounds were identified by GC/MS analysis, including (S,E)-nerolidol (52.5%), τ-cadinol (21.6%) and S-falcarinol (3.6%). Accordingly, the volatile oil (100 g) was subjected to chromatographic separation and purification. As a result, the three compounds, (E)-nerolidol (2 g), τ-cadinol (62 mg) and S-falcarinol (21 mg), were isolated and purified from the volatile oil, the structures of which were unambiguously elucidated by detailed spectroscopic analysis including 1D- and 2D-NMR techniques.
authors.affiliation: Department of Human Anatomy, Histology and Embryology, Institute of Neuroscience, Changsha Medical University, Changsha 410219, China. Shaoli82@aliyun.com.
authors.forename: Li
authors.initials: L
authors.lastname: Shao
authors.affiliation: Department of Human Anatomy, Histology and Embryology, Institute of Neuroscience, Changsha Medical University, Changsha 410219, China. mhbao78@163.com.
authors.forename: Mei-Hua
authors.initials: MH
authors.lastname: Bao
authors.affiliation: Department of Clinical Pharmacology, Xiangya Hospital, Central South University, Changsha 410008, China. Ouyangyj@163.com.
authors.forename: Dong-Sheng
authors.initials: DS
authors.lastname: Ouyang
authors.affiliation: Tang Center for Herbal Medicine Research, The Pritzker School of Medicine, University of Chicago, 5841 South Maryland Avenue, MC 4028, Chicago, IL 60637, USA. CWang@dacc.uchicago.edu.
authors.forename: Chong-Zhi
authors.initials: CZ
authors.lastname: Wang
authors.affiliation: Tang Center for Herbal Medicine Research, The Pritzker School of Medicine, University of Chicago, 5841 South Maryland Avenue, MC 4028, Chicago, IL 60637, USA. CYuan@dacc.uchicago.edu.
authors.forename: Chun-Su
authors.initials: CS
authors.lastname: Yuan
authors.affiliation: Department of Clinical Pharmacology, Xiangya Hospital, Central South University, Changsha 410008, China. hhzhou2003@163.com.
authors.forename: Hong-Hao
authors.initials: HH
authors.lastname: Zhou
authors.affiliation: Department of Clinical Pharmacology, Xiangya Hospital, Central South University, Changsha 410008, China. endeavor34852@aliyun.com.
authors.forename: Wei-Hua
authors.initials: WH
authors.lastname: Huang
mesh_terms: D025924:Chromatography, Supercritical Fluid / Q000379:methods*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D009822:Oils, Volatile / Q000737:chemistry / Q000302:isolation & purification*; D028002:Oplopanax / Q000737:chemistry*; D024301:Plant Bark / Q000737:chemistry*; D018517:Plant Roots / Q000737:chemistry*; D011312:Pressure; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry / Q000379:methods*; D013696:Temperature; D013997:Time Factors
chemical_list: D009822:Oils, Volatile