title: Experimental myocardial infarction induces altered regulatory T cell hemostasis, and adoptive transfer attenuates subsequent remodeling.
abstract: BACKGROUND
  Ischemic cardiac damage is associated with upregulation of cardiac pro-inflammatory cytokines, as well as invasion of lymphocytes into the heart. Regulatory T cells (Tregs) are known to exert a suppressive effect on several immune cell types. We sought to determine whether the Treg pool is influenced by myocardial damage and whether Tregs transfer and deletion affect cardiac remodeling.
  
  
  RESULTS
  The number and functional suppressive activity of Tregs were assayed in mice subjected to experimental myocardial infarction. The numbers of splenocyte-derived Tregs in the ischemic mice were significantly higher after the injury than in the controls, and their suppressive properties were significantly compromised. Compared with PBS, adoptive Treg transfer to mice with experimental infarction reduced infarct size and improved LV remodeling and functional performance by echocardiography. Treg deletion with blocking anti-CD25 antibodies did not influence infarct size or echocardiographic features of cardiac remodeling.
  
  
  CONCLUSIONS
  Treg numbers are increased whereas their function is compromised in mice with that underwent experimental infarction. Transfer of exogeneous Tregs results in attenuation of myocardial remodeling whereas their ablation has no effect. Thus, Tregs may serve as interesting potential interventional targets for attenuating left ventricular remodeling.
authors.affiliation: Heart Center, Kaplan Medical Center, Rehovot, Israel, Affiliated to the Hebrew University, Jerusalem, Israel; Laboratory of Cardiovascular Research, Department of Cardiology, Tel-Aviv Sourasky Medical Center, Tel-Aviv, Israel; Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel.
authors.forename: Rinat
authors.initials: R
authors.lastname: Sharir
authors.affiliation: Heart Center, Kaplan Medical Center, Rehovot, Israel, Affiliated to the Hebrew University, Jerusalem, Israel; Laboratory of Cardiovascular Research, Department of Cardiology, Tel-Aviv Sourasky Medical Center, Tel-Aviv, Israel.
authors.forename: Jonathan
authors.initials: J
authors.lastname: Semo
authors.affiliation: Heart Center, Kaplan Medical Center, Rehovot, Israel, Affiliated to the Hebrew University, Jerusalem, Israel; Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel.
authors.forename: Sara
authors.initials: S
authors.lastname: Shimoni
authors.affiliation: Neufeld Cardiac Research Institute, Tel Aviv University, Sheba Center for Regenerative Medicine, Stem Cells, and Tissue Engineering and Tamman Cardiovascular Research institute, Ramat-Gan, Israel.
authors.forename: Tamar
authors.initials: T
authors.lastname: Ben-Mordechai
authors.affiliation: Neufeld Cardiac Research Institute, Tel Aviv University, Sheba Center for Regenerative Medicine, Stem Cells, and Tissue Engineering and Tamman Cardiovascular Research institute, Ramat-Gan, Israel.
authors.forename: Natalie
authors.initials: N
authors.lastname: Landa-Rouben
authors.affiliation: Heart Center, Kaplan Medical Center, Rehovot, Israel, Affiliated to the Hebrew University, Jerusalem, Israel; Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel.
authors.forename: Sofia
authors.initials: S
authors.lastname: Maysel-Auslender
authors.affiliation: The Bert W. Strassburger Lipid Center, Sheba Medical Center, Tel-Hashomer, Ramat-Gan, Israel.
authors.forename: Aviv
authors.initials: A
authors.lastname: Shaish
authors.affiliation: Heart Center, Kaplan Medical Center, Rehovot, Israel, Affiliated to the Hebrew University, Jerusalem, Israel; Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel.
authors.forename: Michal
authors.initials: M
authors.lastname: Entin-Meer
authors.affiliation: Laboratory of Cardiovascular Research, Department of Cardiology, Tel-Aviv Sourasky Medical Center, Tel-Aviv, Israel; Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel.
authors.forename: Gad
authors.initials: G
authors.lastname: Keren
authors.affiliation: Heart Center, Kaplan Medical Center, Rehovot, Israel, Affiliated to the Hebrew University, Jerusalem, Israel.
authors.forename: Jacob
authors.initials: J
authors.lastname: George
mesh_terms: D019264:Adoptive Transfer; D000818:Animals; D002478:Cells, Cultured; D018920:Coculture Techniques; D004195:Disease Models, Animal; D053645:Interleukin-2 Receptor alpha Subunit / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D015428:Myocardial Reperfusion Injury / Q000276:immunology / Q000503:physiopathology*; D009206:Myocardium / Q000276:immunology* / Q000473:pathology; D013154:Spleen / Q000276:immunology; D050378:T-Lymphocytes, Regulatory / Q000378:metabolism*; D020257:Ventricular Remodeling / Q000276:immunology*
chemical_list: D053645:Interleukin-2 Receptor alpha Subunit