title: Effect of alcohol extract of Acanthus ilicifolius L. on anti-duck hepatitis B virus and protection of liver.
abstract: BACKGROUND
  Acanthus ilicifolius L. is an important medicinal mangrove plant. It is popularly used for its anti-inflammatory, antioxidant activity and hepatoprotective effects. The present study was conducted to evaluate the effect of treatment with alcohol extract of Acanthus ilicifolius L. on duck hepatitis B.
  
  
  METHODS
  One-day-old Guangxi shelducks injected intraperitoneally with strong positive duck hepatitis B virus (DHBV) serum were used to establish a duck hepatitis B animal model in the study. The ducks were respectively administered in different groups with low-, middle- and high-dose alcohol extracts of Acanthus ilicifolius L., the positive control drug acyclovir (ACV) and double-distilled water. The levels of serum DHBV DNA were detected by fluorescence quantitative PCR (FQ-PCR). Duck hepatitis B surface antigen (DHBsAg) and duck hepatitis B e antigen (DHBeAg) OD values in the serum were measured by ELISA. The activity of Alanine Aminotransferase (ALT) and Aspartate Aminotransferase (AST) in the serum was measured, and the livers were taken for histopathological examination.
  
  
  RESULTS
  The levels of serum DHBV DNA and the values of DHBsAg and DHBeAg OD were not significant in any of the dose extract groups. However, the ALT activity was obviously lower in the middle- and high-dose extract groups. It was also found that a high dose of alcohol extract could reduce the activity of AST significantly and significantly improve hepatic pathological effects.
  
  
  CONCLUSIONS
  High-dose alcohol extract of Acanthus ilicifolius L. has an obvious protective effect on the liver function and liver tissue. However, the present study finds that Acanthus ilicifolius L. cannot inhibit the replication of duck hepatitis B virus.
authors.affiliation: Department of Epidemiology, Public Health School, Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China.
authors.forename: Peng-Hai
authors.initials: PH
authors.lastname: Wei
authors.affiliation: Department of Epidemiology, Public Health School, Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China.
authors.forename: Shu-Zhi
authors.initials: SZ
authors.lastname: Wu
authors.affiliation: Department of Epidemiology, Public Health School, Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China.
authors.forename: Xiao-Min
authors.initials: XM
authors.lastname: Mu
authors.affiliation: Department of Epidemiology, Public Health School, Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Xu
authors.affiliation: Department of Epidemiology, Public Health School, Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China.
authors.forename: Qi-Jian
authors.initials: QJ
authors.lastname: Su
authors.affiliation: Department of Epidemiology, Public Health School, Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China.
authors.forename: Jin-Lu
authors.initials: JL
authors.lastname: Wei
authors.affiliation: Department of Epidemiology, Public Health School, Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Department of Epidemiology, Public Health School, Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Qin
authors.affiliation: Department of Epidemiology, Public Health School, Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China. Electronic address: 357154887@qq.com.
authors.forename: Zhi-Chun
authors.initials: ZC
authors.lastname: Xie
mesh_terms: D027901:Acanthaceae / Q000737:chemistry*; D000212:Acyclovir / Q000494:pharmacology / Q000627:therapeutic use; D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D000831:Animals, Newborn; D000998:Antiviral Agents / Q000494:pharmacology / Q000627:therapeutic use; D001219:Aspartate Aminotransferases / Q000097:blood; D004279:DNA, Viral / Q000097:blood; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use*; D004372:Ducks; D018347:Hepadnaviridae Infections / Q000188:drug therapy* / Q000473:pathology; D006514:Hepatitis B Surface Antigens / Q000097:blood; D015611:Hepatitis B Virus, Duck / Q000187:drug effects*; D006513:Hepatitis B e Antigens / Q000097:blood; D006524:Hepatitis, Viral, Animal / Q000188:drug therapy* / Q000473:pathology; D008099:Liver / Q000187:drug effects* / Q000473:pathology; D008517:Phytotherapy; D019562:Viral Load / Q000187:drug effects
chemical_list: D000998:Antiviral Agents; D004279:DNA, Viral; D004365:Drugs, Chinese Herbal; D006514:Hepatitis B Surface Antigens; D006513:Hepatitis B e Antigens; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D000212:Acyclovir
keywords: Acanthus ilicifolius; Alcohol extract; Duck hepatitis B virus