title: Phosphorylation regulates fibrillation of an aggregation core peptide  in the second repeat of microtubule-binding domain of human tau.
abstract: Hyperphosphorylation of the microtubule-associated protein tau is believed to play a crucial role in the neurofibrillary tangles formation in Alzheimer’s disease brain. In this study, fibril formation of peptides containing the critical sequences for tau aggregation VQIINK and a plausible serine phosphorylation site of tau at its C-terminal was investigated. All the peptides formed fibrils with the typical cross-b structural core. However, stability of the fibrils was highly sensitive to the pH conditions for the phosphorylated VQIINK peptide, suggesting a regulatory role of phosphorylation for the amyloid-formation of tau.
authors.forename: Masafumi
authors.initials: M
authors.lastname: Inoue
authors.forename: Shinji
authors.initials: S
authors.lastname: Kaida
authors.forename: Shun
authors.initials: S
authors.lastname: Nakano
authors.forename: Chiara
authors.initials: C
authors.lastname: Annoni
authors.forename: Eiji
authors.initials: E
authors.lastname: Nakata
authors.forename: Takashi
authors.initials: T
authors.lastname: Konno
authors.forename: Takashi
authors.initials: T
authors.lastname: Morii
mesh_terms: D000595:Amino Acid Sequence; D002352:Carrier Proteins / Q000737:chemistry / Q000378:metabolism; D002942:Circular Dichroism; D006801:Humans; D006863:Hydrogen-Ion Concentration; D018625:Microscopy, Atomic Force; D046529:Microscopy, Electron, Transmission; D008870:Microtubules / Q000737:chemistry / Q000378:metabolism*; D016874:Neurofibrillary Tangles; D009842:Oligopeptides / Q000737:chemistry / Q000378:metabolism*; D010455:Peptides / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism*; D010766:Phosphorylation; D011485:Protein Binding; D055550:Protein Stability; D017550:Spectroscopy, Fourier Transform Infrared; D055672:Static Electricity; D016875:tau Proteins / Q000737:chemistry / Q000378:metabolism*
chemical_list: D002352:Carrier Proteins; D009842:Oligopeptides; D010455:Peptides; D016875:tau Proteins; C000710267:tau hexapeptide PHF6