title: Identification of alternatively spliced isoforms of interleukin-2/15 receptor β chain in ducks.
abstract: Interleukin (IL)-2 and IL-15 receptor β (IL-2/15Rβ, CD122) play important roles in signal transduction for biological functions of IL-2 and IL-15. We found that ducks possess three different IL-2/15Rβ transcripts, a conventional form (duIL-2/15Rβ) and two variants. Comparisons between the cDNA and genomic sequences revealed that the two variants, duIL-2/15Rβ-d7 and duIL-2/15Rβ-d9, were novel spliced transcripts resulting from skipping exons 7 and 9, respectively. Expression profiles of duIL-2/15Rβ and its isoforms were examined in healthy tissues, concanavalin A (ConA)-stimulated splenic lymphocytes and in livers and spleens of Riemerella anatipestifer-infected ducks using quantitative real-time PCR (qRT-PCR). Generally, duIL-2/15Rβ-d9 expression was undetectable in healthy tissues, ConA-activated samples, and R. anatipestifer-infected ducks. Expression levels of duIL-2/15Rβ transcript were relatively high to moderate in all healthy tissues tested, while duIL-2/15Rβ-d7 expression was low. Compared to untreated controls, expression levels of duIL-2/15Rβ were elevated in ConA-activated splenic lymphocytes and in livers on day 7 in R. anatipestifer-infected ducks, while duIL-2/15Rβ-d7 expression was unchanged. Additionally, COS-7 cells transfected with duIL-2/15Rβ, duIL-2/15Rβ-d7, or duIL-2/15Rβ-d9 constructs generated 73 kilodalton (kDa), 31kDa, and 40kDa proteins, respectively. This study identified three different IL-2/15Rβ transcripts, including two isoforms generated by alternative splicing and their gene expression patterns in stimulated conditions.
authors.affiliation: College of Veterinary Medicine & Institute of Animal Medicine, Gyeongsang National University, Jinju 660-701, Republic of Korea; Avian Disease Division, Animal and Plant Quarantine Agency, Anyang 430-757, Republic of Korea.
authors.forename: Jipseol
authors.initials: J
authors.lastname: Jeong
authors.affiliation: College of Veterinary Medicine & Institute of Animal Medicine, Gyeongsang National University, Jinju 660-701, Republic of Korea.
authors.forename: Woo H
authors.initials: WH
authors.lastname: Kim
authors.affiliation: College of Veterinary Medicine & Institute of Animal Medicine, Gyeongsang National University, Jinju 660-701, Republic of Korea.
authors.forename: Jaeseung
authors.initials: J
authors.lastname: Yeo
authors.affiliation: College of Veterinary Medicine & Institute of Animal Medicine, Gyeongsang National University, Jinju 660-701, Republic of Korea.
authors.forename: Cherry P
authors.initials: CP
authors.lastname: Fernandez
authors.affiliation: College of Veterinary Medicine & Institute of Animal Medicine, Gyeongsang National University, Jinju 660-701, Republic of Korea.
authors.forename: Suk
authors.initials: S
authors.lastname: Kim
authors.affiliation: Avian Disease Division, Animal and Plant Quarantine Agency, Anyang 430-757, Republic of Korea.
authors.forename: Youn-Jeong
authors.initials: YJ
authors.lastname: Lee
authors.affiliation: Animal Biosciences and Biotechnology Laboratory, Beltsville Agricultural Research Center, ARS, US Department of Agriculture, Beltsville, MD 20705, USA.
authors.forename: Hyun S
authors.initials: HS
authors.lastname: Lillehoj
authors.affiliation: College of Veterinary Medicine & Institute of Animal Medicine, Gyeongsang National University, Jinju 660-701, Republic of Korea. Electronic address: wongimin@gnu.ac.kr.
authors.forename: Wongi
authors.initials: W
authors.lastname: Min
mesh_terms: D017398:Alternative Splicing / Q000235:genetics / Q000276:immunology*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004372:Ducks / Q000235:genetics / Q000276:immunology*; D053646:Interleukin-2 Receptor beta Subunit / Q000235:genetics / Q000276:immunology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D020033:Protein Isoforms / Q000235:genetics / Q000276:immunology; D012313:RNA / Q000737:chemistry / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
chemical_list: D053646:Interleukin-2 Receptor beta Subunit; D020033:Protein Isoforms; D012313:RNA
keywords: Duck; IL-2/15Rβ; Isoforms; Riemerella anatipestifer infection; gene expression