title: DNA methylation dynamics of a maternally methylated DMR in the mouse Dlk1-Dio3 domain.
abstract: The mouse delta-like homolog 1 and type III iodothyronine deiodinase (Dlk1-Dio3) imprinted domain contains three known paternally methylated differentially methylated regions (DMRs): intergenic DMR (IG-DMR), maternally expressed 3-DMR (Gtl2-DMR), and Dlk1-DMR. Here, we report the first maternally methylated DMR, CpG island 2 (CGI-2), is located approximately 800 bp downstream of miR-1188. CGI-2 is highly methylated in sperm and oocytes, de-methylated in pre-implantation embryos, and differentially re-methylated during post-implantation development. CGI-2, similarly to Gtl2-DMR and Dlk1-DMR, acquires differential methylation prior to embryonic day 7.5 (E7.5). Both H3K4me3 and H3K9me3 histone modifications are enriched at CGI-2. Furthermore, CCCTC-binding factor (CTCF) binds to both alleles of CGI-2 in vivo. These results contribute to the investigation of imprinting regulation in this domain.
authors.affiliation: School of Life Science and Technology, State Key Laboratory of Urban Water Resources and Environment, Harbin Institute of Technology, Harbin 150080, Heilongjiang, China.
authors.forename: Tie-Bo
authors.initials: TB
authors.lastname: Zeng
authors.affiliation: School of Life Science and Technology, State Key Laboratory of Urban Water Resources and Environment, Harbin Institute of Technology, Harbin 150080, Heilongjiang, China.
authors.forename: Hong-Juan
authors.initials: HJ
authors.lastname: He
authors.affiliation: School of Life Science and Technology, State Key Laboratory of Urban Water Resources and Environment, Harbin Institute of Technology, Harbin 150080, Heilongjiang, China.
authors.forename: Zheng-Bin
authors.initials: ZB
authors.lastname: Han
authors.affiliation: School of Life Science and Technology, State Key Laboratory of Urban Water Resources and Environment, Harbin Institute of Technology, Harbin 150080, Heilongjiang, China.
authors.forename: Feng-Wei
authors.initials: FW
authors.lastname: Zhang
authors.affiliation: School of Life Science and Technology, State Key Laboratory of Urban Water Resources and Environment, Harbin Institute of Technology, Harbin 150080, Heilongjiang, China.
authors.forename: Zhi-Jun
authors.initials: ZJ
authors.lastname: Huang
authors.affiliation: School of Life Science and Technology, State Key Laboratory of Urban Water Resources and Environment, Harbin Institute of Technology, Harbin 150080, Heilongjiang, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Liu
authors.affiliation: School of Life Science and Technology, State Key Laboratory of Urban Water Resources and Environment, Harbin Institute of Technology, Harbin 150080, Heilongjiang, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Cui
authors.affiliation: School of Life Science and Technology, State Key Laboratory of Urban Water Resources and Environment, Harbin Institute of Technology, Harbin 150080, Heilongjiang, China. Electronic address: kigo@hit.edu.cn.
authors.forename: Qiong
authors.initials: Q
authors.lastname: Wu
mesh_terms: D000818:Animals; D001483:Base Sequence; D000076246:CCCTC-Binding Factor; D002135:Calcium-Binding Proteins; D018899:CpG Islands / Q000235:genetics; D019175:DNA Methylation; D010064:Embryo Implantation; D047108:Embryonic Development / Q000235:genetics; D005260:Female; D018392:Genomic Imprinting; D006657:Histones / Q000737:chemistry / Q000378:metabolism; D036341:Intercellular Signaling Peptides and Proteins / Q000235:genetics*; D007438:Introns / Q000235:genetics; D007453:Iodide Peroxidase / Q000235:genetics*; D051379:Mice; D035683:MicroRNAs / Q000235:genetics; D009035:Mothers; D011247:Pregnancy; D012097:Repressor Proteins / Q000378:metabolism
chemical_list: D000076246:CCCTC-Binding Factor; D002135:Calcium-Binding Proteins; C000619698:Ctcf protein, mouse; C512925:Dlk1 protein, mouse; D006657:Histones; D036341:Intercellular Signaling Peptides and Proteins; D035683:MicroRNAs; D012097:Repressor Proteins; C117274:iodothyronine deiodinase type III; D007453:Iodide Peroxidase
keywords: CCCTC-binding factor; DNA methylation; Differentially methylated region; Dlk1–Dio3 domain; Genomic imprinting