title: Background eliminated signal-on electrochemical aptasensing platform for highly sensitive detection of protein.
abstract: Using platelet-derived growth factor B chain dimer (PDGF-BB) as the model target, a background current eliminated electrochemical aptameric sensing platform for highly sensitive and signal-on detection of protein is proposed in this paper. Successful fabrication of the biosensor depends on ingenious design of aptamer probe, which contains the aptamer sequence for PDGF-BB and the recognition sequence for EcoRI endonuclease. In the absence of PDGF-BB, the ferrocene labeled aptamer probe folds into a hairpin structure and forms a recognition site for EcoRI. By treatment with endonuclease, the specific and cleavable double-stranded region is cut off and redox-active ferrocene molecule is removed from the electrode surface, and almost no peak current is observed. When binding with target protein, the designed aptamer probe changes its conformation and dissociates the recognition double strand. The integrated aptamer probe is maintained when exposing to EcoRI endonuclease, resulting in obvious peak current. Therefore, a signal-on and sensitive sensing strategy for PDGF-BB detection is fabricated with eliminated background current. Under the optimized experimental conditions, a wide linear response range of 4 orders of magnitude from 20pgmL(-1) to 200ngmL(-1) is achieved with a detection limit of 10pgmL(-1). Moreover, the present aptameric platform is universal for the analysis of a broad range of target molecules of interest by changing and designing the sequence of aptamer probe.
authors.affiliation: Department of Chemistry and Chemical Engineering, Hunan University of Arts and Science, Changde 415000, PR China; State Key Laboratory for Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, PR China. Electronic address: zsb0119@163.com.
authors.forename: Songbai
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Department of Chemistry and Chemical Engineering, Hunan University of Arts and Science, Changde 415000, PR China.
authors.forename: Xia
authors.initials: X
authors.lastname: Hu
authors.affiliation: Department of Chemistry and Chemical Engineering, Hunan University of Arts and Science, Changde 415000, PR China.
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Yang
authors.affiliation: Department of Chemistry and Chemical Engineering, Hunan University of Arts and Science, Changde 415000, PR China.
authors.forename: Qinli
authors.initials: Q
authors.lastname: Sun
authors.affiliation: Department of Chemistry and Chemical Engineering, Hunan University of Arts and Science, Changde 415000, PR China.
authors.forename: Xiaolin
authors.initials: X
authors.lastname: Xu
authors.affiliation: Department of Chemistry and Chemical Engineering, Hunan University of Arts and Science, Changde 415000, PR China.
authors.forename: Xuewen
authors.initials: X
authors.lastname: Liu
authors.affiliation: Department of Chemistry and Chemical Engineering, Hunan University of Arts and Science, Changde 415000, PR China.
authors.forename: Guangyu
authors.initials: G
authors.lastname: Shen
authors.affiliation: Department of Chemistry and Chemical Engineering, Hunan University of Arts and Science, Changde 415000, PR China.
authors.forename: Jilin
authors.initials: J
authors.lastname: Lu
authors.affiliation: State Key Laboratory for Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, PR China.
authors.forename: Guoli
authors.initials: G
authors.lastname: Shen
authors.affiliation: State Key Laboratory for Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, PR China.
authors.forename: Ruqin
authors.initials: R
authors.lastname: Yu
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry* / Q000378:metabolism; D001483:Base Sequence; D000077214:Becaplermin; D015374:Biosensing Techniques / Q000379:methods*; D015246:Deoxyribonuclease EcoRI / Q000378:metabolism; D004563:Electrochemistry / Q000379:methods*; D006801:Humans; D057230:Limit of Detection; D020574:Proto-Oncogene Proteins c-sis / Q000032:analysis / Q000097:blood*
chemical_list: D052157:Aptamers, Nucleotide; D020574:Proto-Oncogene Proteins c-sis; D000077214:Becaplermin; D015246:Deoxyribonuclease EcoRI
keywords: Aptamer; Biosensor; Electrochemical; Endonuclease; PDGF-BB