title: Apoptotic effects and glucose-6-phosphate dehydrogenase responses in liver and gill tissues of rainbow trout treated with chlorpyrifos.
abstract: We investigated apoptotic effects and changes in glucose-6-phosphate dehydrogenase (G6PD) enzyme activity in liver and gill tissues of fish exposed to chlorpyrifos. Three different chlorpyrifos doses (2.25, 4.5 and 6.75 μg/L) were administrated to rainbow trout at different time intervals (24, 48, 72 and 96 h). Acute exposure to chlorpyrifos showed time dependent decrease in G6PD enzyme activity at all concentrations (p < 0.05). Immunohistochemical results showed that chlorpyrifos caused mucous cell loss in gill tissue and apoptosis via caspase-3 activation in fish. The present study suggested that chlorpyrifos inhibits G6PD enzyme and causes mucous cell loss in gill and apoptosis in gill and liver tissues.
authors.affiliation: Department of Basic Sciences, Faculty of Fisheries, Ataturk University, TR-25240 Erzurum, Turkey. drahmettopal@hotmail.com
authors.forename: Ahmet
authors.initials: A
authors.lastname: Topal
authors.forename: Muhammed
authors.initials: M
authors.lastname: Atamanalp
authors.forename: Ertan
authors.initials: E
authors.lastname: Oruç
authors.forename: Muammer
authors.initials: M
authors.lastname: Kırıcı
authors.forename: Esat Mahmut
authors.initials: EM
authors.lastname: Kocaman
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D004390:Chlorpyrifos / Q000633:toxicity*; D005880:Gills / Q000187:drug effects / Q000201:enzymology; D005954:Glucosephosphate Dehydrogenase / Q000096:biosynthesis* / Q000187:drug effects; D008099:Liver / Q000187:drug effects / Q000201:enzymology; D017686:Oncorhynchus mykiss; D010084:Oxidation-Reduction; D014874:Water Pollutants, Chemical / Q000633:toxicity
chemical_list: D014874:Water Pollutants, Chemical; D005954:Glucosephosphate Dehydrogenase; D004390:Chlorpyrifos