title: A sensitive sequential 'on/off' SERS assay for heparin with wider detection window and higher reliability based on the reversed surface charge changes of functionalized Au@Ag nanoparticles.
abstract: A sequential 'on/off' dual mode SERS assay platform for heparin with wider detection window and higher reliability is constructed based on electrostatic forces, in which the highly protonated chitosan encapsulated p-Mercaptobenzoic acid coated Au@Ag core-shell nanoparticles undergo sequential aggregation/segregation upon the additive of heparin with a limit of detection of 43.74ng/mL (5.69U/mL) and a continuous concentration range of 50-800ng/mL (6.5-104U/mL), which are lower in sensitivity and wider in detection window than the most reported assay for heparin. Remarkably, the latter declined window over a range of 350-800ng/mL in contrast, which has not reported before, is extremely important in reliable and practical assay of heparin.
authors.affiliation: Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Zeng
authors.affiliation: Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, China.
authors.forename: Jin-Ju
authors.initials: JJ
authors.lastname: Pei
authors.affiliation: Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, China.
authors.forename: Li-Hua
authors.initials: LH
authors.lastname: Wang
authors.affiliation: Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, China. Electronic address: agshen@whu.edu.cn.
authors.forename: Ai-Guo
authors.initials: AG
authors.lastname: Shen
authors.affiliation: Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, China. Electronic address: jmhu@whu.edu.cn.
authors.forename: Ji-Ming
authors.initials: JM
authors.lastname: Hu
mesh_terms: D000925:Anticoagulants / Q000032:analysis / Q000097:blood*; D015374:Biosensing Techniques / Q000379:methods; D048271:Chitosan / Q000737:chemistry; D006046:Gold / Q000737:chemistry*; D006493:Heparin / Q000032:analysis / Q000097:blood*; D006801:Humans; D057230:Limit of Detection; D053768:Metal Nanoparticles / Q000737:chemistry*; D015203:Reproducibility of Results; D012834:Silver / Q000737:chemistry*; D013059:Spectrum Analysis, Raman / Q000379:methods*; D055672:Static Electricity; D013499:Surface Properties
chemical_list: D000925:Anticoagulants; D012834:Silver; D006046:Gold; D006493:Heparin; D048271:Chitosan
keywords: Aggregation/segregation; Au@Ag core–shell nanoparticles; Heparin; SERS