title: Quantitative analyses of the effect of silk fibroin/nano-hydroxyapatite composites on osteogenic differentiation of MG-63 human osteosarcoma cells.
abstract: Silk fibroin (SF)/nano-hydroxyapatite (n-HA) composites are potential biomaterials for bone defect repair. Up to now, the biological evaluation studies of SF/n-HA composites have primarily concentrated on their biocompatibility at cell level such as cell viability and proliferation and tissue level such as material absorption and new bone formation. In this work, SF/n-HA composites were fabricated using a simplified coprecipitation methods and were deposited onto Ti alloy substrates. Then the cell adhesion ability of SF/n-HA composites was observed by SEM and cell proliferation ability of SF/n-HA composites was determined by MTT assay. The ALP activity, BGP contents, and Col I contents of MG-63 human osteosarcoma cells on SF/n-HA composites were quantitatively analyzed. HA nanocrystals were used as controls. These experiments showed that SF/n-HA composites had better cell adhesion and osteogenic differentiation abilities than n-HA materials. This work provides quantitative data to analyze the effect of SF/n-HA composites on cell osteogenic differentiation.
authors.affiliation: Shandong Liaocheng City Second People's Hospital, Taishan Medical University, Shandong Province 252601, People's Republic of China.
authors.forename: Linxue
authors.initials: L
authors.lastname: Lin
authors.affiliation: Department of Radiology, Jinan No. 4 People's Hospital, Shandong Province 250031, People's Republic of China.
authors.forename: Runsong
authors.initials: R
authors.lastname: Hao
authors.affiliation: Porton Fine Chemicals Ltd, Chongqing 401121, People's Republic of China.
authors.forename: Wei
authors.initials: W
authors.lastname: Xiong
authors.affiliation: National Engineering Research Center for Nanotechnology, Shanghai 200241, People's Republic of China. Electronic address: jianzhongpku@hotmail.com.
authors.forename: Jian
authors.initials: J
authors.lastname: Zhong
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry / Q000494:pharmacology; D002448:Cell Adhesion / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D011232:Chemical Precipitation; D017886:Durapatite / Q000737:chemistry* / Q000494:pharmacology*; D005349:Fibroins / Q000737:chemistry / Q000494:pharmacology* / Q000648:ultrastructure; D006801:Humans; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D010012:Osteogenesis / Q000187:drug effects*; D012516:Osteosarcoma / Q000473:pathology*; D047011:Silk / Q000737:chemistry / Q000494:pharmacology* / Q000648:ultrastructure
chemical_list: D001672:Biocompatible Materials; D047011:Silk; D005349:Fibroins; D017886:Durapatite
keywords: Bone defect repair; Cell proliferation; MG-63 human osteosarcoma cells; Osteogenic differentiation; Silk fibroin/nano-hydroxyapatite composites