title: The effects of D-allose on transient ischemic neuronal death and analysis of its mechanism.
abstract: The present study investigates the neuroprotective effects of d-allose, a rare sugar, against ischemia/reperfusion injury in a gerbil model. Transient forebrain ischemia was induced by occlusion of the bilateral common carotid arteries for 5 min. D-Allose was intravenously injected before and after ischemia (200 mg/kg). Extracellular glutamate and lactate release from the gerbil brain, and PO₂ profiles were monitored during ischemia and reperfusion. We also examined neuronal death and oxidative damage in the hippocampus one week after ischemia reperfusion, and investigated functional outcome. D-Allose administration suppressed glutamate and lactate release compared to vehicle controls. Brain damage, 8-OHdG levels (a marker of oxidative stress) and locomotor activities were significantly decreased by D-allose treatment. The present results suggest that d-allose reduces delayed neuronal death and behavioral deficits after transient ischemia by changing cerebral metabolism and inhibiting oxidative stress.
authors.affiliation: Department of Molecular Neurobiology, Kagawa University Faculty of Medicine, Miki, Japan.
authors.forename: Yanan
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Molecular Neurobiology, Kagawa University Faculty of Medicine, Miki, Japan; Department of Neurological Surgery, Kagawa University Faculty of Medicine, Miki, Japan. Electronic address: tanakamu@kms.ac.jp.
authors.forename: Takehiro
authors.initials: T
authors.lastname: Nakamura
authors.affiliation: Department of Molecular Neurobiology, Kagawa University Faculty of Medicine, Miki, Japan.
authors.forename: Tetsuhiko
authors.initials: T
authors.lastname: Toyoshima
authors.affiliation: Department of Neurological Surgery, Kagawa University Faculty of Medicine, Miki, Japan.
authors.forename: Aya
authors.initials: A
authors.lastname: Shinomiya
authors.affiliation: Department of Neurological Surgery, Kagawa University Faculty of Medicine, Miki, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Tamiya
authors.affiliation: Department of Cell Physiology, Kagawa University Faculty of Medicine, Miki, Japan.
authors.forename: Masaaki
authors.initials: M
authors.lastname: Tokuda
authors.affiliation: Department of Neurosurgery, University of Michigan, Ann Arbor, MI, USA.
authors.forename: Richard F
authors.initials: RF
authors.lastname: Keep
authors.affiliation: Department of Molecular Neurobiology, Kagawa University Faculty of Medicine, Miki, Japan.
authors.forename: Toshifumi
authors.initials: T
authors.lastname: Itano
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D000704:Analysis of Variance; D000818:Animals; D002540:Cerebral Cortex / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D004249:DNA Damage / Q000187:drug effects; D003849:Deoxyguanosine / Q000031:analogs & derivatives; D004195:Disease Models, Animal; D004334:Drug Administration Schedule; D005849:Gerbillinae; D005947:Glucose / Q000494:pharmacology* / Q000627:therapeutic use; D018698:Glutamic Acid / Q000378:metabolism; D002546:Ischemic Attack, Transient / Q000150:complications / Q000188:drug therapy / Q000473:pathology*; D019344:Lactic Acid / Q000378:metabolism; D008297:Male; D017551:Microdialysis; D009069:Movement Disorders / Q000209:etiology; D009474:Neurons / Q000187:drug effects*; D010100:Oxygen / Q000378:metabolism; D013997:Time Factors
chemical_list: D019344:Lactic Acid; D018698:Glutamic Acid; C002055:allose; D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine; D005947:Glucose; D010100:Oxygen
keywords: Glutamate; Lactate; Oxidation; Reperfusion; Transient forebrain ischemia; d-Allose