title: Untangling the effects of peptide sequences and nanotopographies in a biomimetic niche for directed differentiation of iPSCs by assemblies of genetically engineered viral nanofibers.
abstract: Here we report the design of a unique matrix, assembled from engineered M13 phage bionanofibers with specific cues of nanotopographies and versatile signal peptides to simulate native niche for directing the fate of induced pluripotent stem cells (iPSCs). By independently varying the peptide sequences and nanotopographies, we find that the resident iPSCs on the phage matrix are first differentiated into mesenchymal progenitor cells (MPCs), which are further differentiated into osteoblasts in the absence of osteogenic supplements due to the elongation induced by phage nanofibers. The phage-based matrix represents not only a biomimetic stem cell niche enabling independently varying biochemical and biophysical cues in one system but also a substrate for generating a safe and efficient cell source for tissue engineering.
authors.affiliation: Department of Chemistry and Biochemistry, Stephenson Life Sciences Research Center, University of Oklahoma, Norman, Oklahoma 73019, United States.
authors.forename: Jianglin
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Chemistry and Biochemistry, Stephenson Life Sciences Research Center, University of Oklahoma, Norman, Oklahoma 73019, United States.
authors.forename: Lin
authors.initials: L
authors.lastname: Wang
authors.affiliation: Institute of Applied Bioresource Research, College of Animal Science, Zhejiang University, Yuhangtang Road 866, Hangzhou, Zhejiang 310058, China.
authors.forename: Mingying
authors.initials: M
authors.lastname: Yang
authors.affiliation: Department of Chemistry and Biochemistry, Stephenson Life Sciences Research Center, University of Oklahoma, Norman, Oklahoma 73019, United States.
authors.forename: Ye
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: Materials Science Division, Lawrence Berkeley National Laboratory, One Cyclotron Road, Berkeley, California 94720, United States.
authors.forename: Antoni
authors.initials: A
authors.lastname: Tomsia
authors.affiliation: Department of Chemistry and Biochemistry, Stephenson Life Sciences Research Center, University of Oklahoma, Norman, Oklahoma 73019, United States.
authors.forename: Chuanbin
authors.initials: C
authors.lastname: Mao
mesh_terms: D017104:Bacteriophage M13 / Q000737:chemistry* / Q000235:genetics / Q000648:ultrastructure; D040761:Biomimetic Materials / Q000138:chemical synthesis*; D002454:Cell Differentiation; D002460:Cell Line; D061505:Cell Surface Display Techniques; D005109:Extracellular Matrix / Q000737:chemistry / Q000648:ultrastructure; D005818:Genetic Engineering / Q000379:methods; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology* / Q000502:physiology; D008422:Materials Testing; D057139:Nanofibers / Q000737:chemistry* / Q000648:ultrastructure; D010006:Osteoblasts / Q000166:cytology* / Q000502:physiology; D010012:Osteogenesis / Q000502:physiology; D010316:Particle Size; D055153:Stem Cell Niche / Q000502:physiology*; D013499:Surface Properties; D023822:Tissue Engineering / Q000379:methods
keywords: Stem cell niche; bionanofiber; peptides; phage display; topography; viruses