title: New water-soluble copper (II) complexes including 4,7-dimethyl-1,10-phenanthroline and L-tyrosine: synthesis, characterization, DNA interactions and cytotoxicities.
abstract: Two new water-soluble copper(II) complexes, [Cu(dmphen)2(NO3)]NO3 (1), [Cu(dmphen)(tyr)(H2O)]NO3·H2O (2) and the diquarternary salt of dmphen (dmphen = 4,7-dimethyl-1,10-phenanthroline and tyr = L-tyrosine), have been synthesized and characterized by elemental analysis, (1)H NMR, (13)C NMR and IR spectroscopy, thermal analysis and single crystal X-ray diffraction techniques. The CT-DNA binding properties of these compounds have been investigated by absorption, emission spectroscopy and thermal denaturation measurements. The supercoiled pBR322 plasmid DNA cleavage activity of these compounds has been explored by agarose gel electrophoresis. The cytotoxicity of these compounds against MCF-7, Caco-2, A549 cancer cells and BEAS-2B healthy cells was also studied by the XTT method. Complexes 1 and 2 exhibit significant cytotoxicity, with lower IC50 values than those of cisplatin.
authors.affiliation: Department of Chemistry, Faculty of Arts and Sciences, Uludag University, 16059 Bursa, Turkey.
authors.forename: Duygu
authors.initials: D
authors.lastname: İnci
authors.affiliation: Department of Chemistry, Faculty of Arts and Sciences, Uludag University, 16059 Bursa, Turkey. Electronic address: rahmiye@uludag.edu.tr.
authors.forename: Rahmiye
authors.initials: R
authors.lastname: Aydın
authors.affiliation: Department of Biology, Faculty of Arts and Sciences, Uludag University, 16059 Bursa, Turkey.
authors.forename: Dilek
authors.initials: D
authors.lastname: Yılmaz
authors.affiliation: Department of Chemistry, Faculty of Arts and Sciences, Uludag University, 16059 Bursa, Turkey.
authors.forename: Hasene Mutlu
authors.initials: HM
authors.lastname: Gençkal
authors.affiliation: Department of Biology, Faculty of Arts and Sciences, Uludag University, 16059 Bursa, Turkey.
authors.forename: Özgür
authors.initials: Ö
authors.lastname: Vatan
authors.affiliation: Department of Biology, Faculty of Arts and Sciences, Uludag University, 16059 Bursa, Turkey.
authors.forename: Nilüfer
authors.initials: N
authors.lastname: Çinkılıç
authors.affiliation: Department of Chemistry, Faculty of Science, Gebze Institute of Technology, 41400 Kocaeli, Turkey.
authors.forename: Yunus
authors.initials: Y
authors.lastname: Zorlu
mesh_terms: D000970:Antineoplastic Agents / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D056831:Coordination Complexes / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology; D003300:Copper / Q000737:chemistry* / Q000494:pharmacology; D018360:Crystallography, X-Ray; D004247:DNA / Q000378:metabolism; D053837:DNA Cleavage / Q000187:drug effects; D006801:Humans; D007364:Intercalating Agents / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology; D008958:Models, Molecular; D009369:Neoplasms / Q000188:drug therapy / Q000378:metabolism; D010618:Phenanthrolines / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology; D014443:Tyrosine / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology
chemical_list: D000970:Antineoplastic Agents; D056831:Coordination Complexes; D007364:Intercalating Agents; D010618:Phenanthrolines; D014443:Tyrosine; D003300:Copper; D004247:DNA; C025205:1,10-phenanthroline
keywords: 4,7-Dimethyl-1,10-phenanthroline; Copper(II); Cytotoxicity; DNA binding and cleavage; Single crystal X-ray diffraction; l-tyrosine