title: Purification, characterization and anti-diabetic activity of a polysaccharide from mulberry leaf.
abstract: In the present study, a high-purity polysaccharide from mulberry leaf (MLP) was purified and characterized, and its anti-diabetic effects were investigated in streptozotocin (STZ)-induced diabetic rats. Our results showed that the obtained MLP (purity 99.8%) was determined to be composed of d-arabinose, d-xylose, d-glucose, d-rhamnose and d-mannose with molar ratio of 1:2.13:6.53:1.04:8.73. Oral administration of MLP at 50-200mg/kgbodyweight daily for 5weeks significantly reduced the levels of fasting blood glucose (FBG), glycosylated serum protein (GSP), serum total cholesterol (TC), and serum triglyceride (TG), and increased the body weight, fasting insulin (FINS), C-peptide (C-P), liver glycogen, liver glucokinase, and serum high-density lipoprotein cholesterol (HDL-C). Moreover, MLP promoted marked pancreatic β-cell regeneration and insulin secretion, and reduced liver fat accumulation in diabetic rats. The treatment effect of MLP on diabetes was similar to the effect of antidiabetic drug glibenclamide. These results clearly indicated that MLP may have a potential for the treatment of hyperglycemia and hyperlipidemia in diabetes.
authors.affiliation: College of Forestry, Shandong Agricultural University, Taian 271018, China; College of Life Science, Shandong Agricultural University, Taian 271018, China.
authors.forename: Yao
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: College of Forestry, Shandong Agricultural University, Taian 271018, China.
authors.forename: Chunjiu
authors.initials: C
authors.lastname: Ren
authors.affiliation: College of Forestry, Shandong Agricultural University, Taian 271018, China.
authors.forename: Guobing
authors.initials: G
authors.lastname: Lu
authors.affiliation: College of Forestry, Shandong Agricultural University, Taian 271018, China.
authors.forename: Weizheng
authors.initials: W
authors.lastname: Cui
authors.affiliation: College of Forestry, Shandong Agricultural University, Taian 271018, China. Electronic address: lz20011022@126.com.
authors.forename: Zhimei
authors.initials: Z
authors.lastname: Mu
authors.affiliation: College of Forestry, Shandong Agricultural University, Taian 271018, China.
authors.forename: Huiju
authors.initials: H
authors.lastname: Gao
authors.affiliation: College of Forestry, Shandong Agricultural University, Taian 271018, China.
authors.forename: Yanwen
authors.initials: Y
authors.lastname: Wang
mesh_terms: D000818:Animals; D001786:Blood Glucose / Q000032:analysis; D002784:Cholesterol / Q000097:blood; D003921:Diabetes Mellitus, Experimental / Q000097:blood / Q000188:drug therapy / Q000473:pathology; D059305:Diet, High-Fat; D006943:Hyperglycemia / Q000097:blood / Q000188:drug therapy* / Q000473:pathology; D006949:Hyperlipidemias / Q000097:blood / Q000188:drug therapy* / Q000473:pathology; D007004:Hypoglycemic Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*; D000960:Hypolipidemic Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*; D007328:Insulin / Q000097:blood; D008099:Liver / Q000187:drug effects / Q000473:pathology; D008297:Male; D031625:Morus; D010179:Pancreas / Q000187:drug effects / Q000473:pathology; D008517:Phytotherapy; D018515:Plant Leaves; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*; D017208:Rats, Wistar; D014280:Triglycerides / Q000097:blood
chemical_list: D001786:Blood Glucose; D007004:Hypoglycemic Agents; D000960:Hypolipidemic Agents; D007328:Insulin; D011134:Polysaccharides; D014280:Triglycerides; D002784:Cholesterol
keywords: Diabetes mellitus; Hypoglycemic; Hypolipidemic; Mulberry leaf; Polysaccharide