title: Interaction and enrichment of protein on cationic polysaccharide surfaces.
abstract: In this study, the interaction of fluorescein isothiocyanate functionalized bovine serum albumin (FITC-BSA) with cellulose surfaces decorated with trimethyl chitosan (TMC) is investigated. Two types of TMC, one exhibiting a lower and one with a higher degree of cationization are used for protein adsorption. The adsorption is carried out at different pH values and concentrations of the protein solution. The amount, morphology and wettability of FITC-BSA coating on TMC/cellulose films are determined using quartz crystal microbalance with dissipation (QCM-D), atomic force microscopy, fluorescence microscopy and contact angle measurements. A lower pH and higher concentration of protein solution resulted in a greater amount of irreversibly adsorbed material owing to the reduced solubility and minimized electrostatic repulsion. A maximum adsorption of protein is observed on cellulose surfaces functionalized with TMC carrying a higher degree of cationization compared to TMC with a lower degree of cationization and pure cellulose surfaces at all applied concentrations and pH values. BSA is a commonly used model protein and is applied in this study to better understand its interaction with cationically rendered cellulose surfaces. Such knowledge is essential for creation of multifunctional polysaccharide-based biomaterials.
authors.affiliation: Institute for Chemistry, University of Graz, Heinrichstrasse 28, 8010 Graz, Austria. Electronic address: tamilselvan.mohan@uni-graz.at.
authors.forename: Tamilselvan
authors.initials: T
authors.lastname: Mohan
authors.affiliation: Institute for Chemistry, University of Graz, Heinrichstrasse 28, 8010 Graz, Austria.
authors.forename: Gerald
authors.initials: G
authors.lastname: Findenig
authors.affiliation: Institute for Chemistry, University of Graz, Heinrichstrasse 28, 8010 Graz, Austria.
authors.forename: Stefan
authors.initials: S
authors.lastname: Höllbacher
authors.affiliation: Institute for Chemistry, University of Graz, Heinrichstrasse 28, 8010 Graz, Austria.
authors.forename: Christoph
authors.initials: C
authors.lastname: Cerny
authors.affiliation: Institute for the Engineering and Design of Materials, University of Maribor, Smetanova 17, 2000 Maribor, Slovenia.
authors.forename: Tijana
authors.initials: T
authors.lastname: Ristić
authors.affiliation: Institute for the Engineering and Design of Materials, University of Maribor, Smetanova 17, 2000 Maribor, Slovenia.
authors.forename: Rupert
authors.initials: R
authors.lastname: Kargl
authors.affiliation: Institute for the Engineering and Design of Materials, University of Maribor, Smetanova 17, 2000 Maribor, Slovenia; Institute for Chemistry and Technology of Materials, Graz University of Technology, Stremayrgasse 9, 8010 Graz, Austria.
authors.forename: Stefan
authors.initials: S
authors.lastname: Spirk
authors.affiliation: Faculty of Medicine, University of Maribor, Taborska ulica 8, 2000 Maribor, Slovenia.
authors.forename: Uros
authors.initials: U
authors.lastname: Maver
authors.affiliation: Institute for the Engineering and Design of Materials, University of Maribor, Smetanova 17, 2000 Maribor, Slovenia.
authors.forename: Karin
authors.initials: K
authors.lastname: Stana-Kleinschek
authors.affiliation: Institute for Chemistry, University of Graz, Heinrichstrasse 28, 8010 Graz, Austria.
authors.forename: Volker
authors.initials: V
authors.lastname: Ribitsch
mesh_terms: D000588:Amines / Q000737:chemistry*; D000818:Animals; D002417:Cattle; D048271:Chitosan / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D008856:Microscopy, Fluorescence; D011134:Polysaccharides / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry*; D017155:Wettability
chemical_list: D000588:Amines; C118071:N-trimethyl chitosan chloride; D011134:Polysaccharides; D011506:Proteins; D048271:Chitosan
keywords: AFM; Cellulose surface; Fluorescence microscope; Protein adsorption; QCM-D; Trimethyl chitosan