title: Preparation and evaluation of a novel liposomal formulation of cisplatin.
abstract: A novel liposomal formulation of cisplatin (L-CDDP) was synthesized and characterized. The L-CDDP was formed by conjugating CDDP to the carboxyl of oleic acid incorporated into empty liposomes. Particle size (155.4±16.1nm) and zeta potential (-50.92±1.19mV) of the L-CDDP were determined. In addition, pharmacokinetic properties and antitumor activity in vitro and in vivo were evaluated. Pharmacokinetic study demonstrated that L-CDDP had markedly prolonged circulation time relative to the free drug. Furthermore, L-CDDP showed significantly enhanced in vitro cytotoxicity in comparison to free CDDP. A549-engrafted mice treated with L-CDDP had a higher survival rate compared to those treated with free CDDP. Finally, A549-engrafted mice treated with L-CDDP showed no significant loss of body weight, whereas free CDDP treatment at the same dose caused significant loss of body weight. These results suggest further evaluation of the in vivo antitumor efficacy of the novel L-CDDP formulation is warranted.
authors.affiliation: State Key Laboratory of Virology, Ministry of Education Key Laboratory of Combinatorial Biosynthesis and Drug Discovery, Wuhan University School of Pharmaceutical Sciences, Wuhan 430071, PR China; Division of Pharmaceutics, The Ohio State University, Columbus, OH 43210, United States.
authors.forename: Xiaoju
authors.initials: X
authors.lastname: Zhou
authors.affiliation: Wuhan Grand Hoyo Pharmaceutical Co., Ltd, Wuhan 430074, PR China.
authors.forename: Jiong
authors.initials: J
authors.lastname: Wang
authors.affiliation: Division of Pharmaceutics, The Ohio State University, Columbus, OH 43210, United States.
authors.forename: Jianhong
authors.initials: J
authors.lastname: Wu
authors.affiliation: Division of Pharmaceutics, The Ohio State University, Columbus, OH 43210, United States.
authors.forename: Xiaojuan
authors.initials: X
authors.lastname: Yang
authors.affiliation: Division of Pharmaceutics, The Ohio State University, Columbus, OH 43210, United States.
authors.forename: Bryant C
authors.initials: BC
authors.lastname: Yung
authors.affiliation: NSF Nanoscale Science and Engineering Center (NSEC) for Affordable Nanoengineering of Polymeric Biomedical Devices (CANPBD), The Ohio State University, Columbus, OH 43210, United States.
authors.forename: L James
authors.initials: LJ
authors.lastname: Lee
authors.affiliation: Division of Pharmaceutics, The Ohio State University, Columbus, OH 43210, United States; NSF Nanoscale Science and Engineering Center (NSEC) for Affordable Nanoengineering of Polymeric Biomedical Devices (CANPBD), The Ohio State University, Columbus, OH 43210, United States. Electronic address: lee.1339@osu.edu.
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Lee
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000008:administration & dosage / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D002626:Chemistry, Pharmaceutical; D002945:Cisplatin / Q000008:administration & dosage / Q000737:chemistry / Q000493:pharmacokinetics* / Q000494:pharmacology*; D004304:Dosage Forms; D006801:Humans; D008081:Liposomes / Q000737:chemistry*; D051379:Mice; D008813:Mice, Inbred ICR; D008819:Mice, Nude; D009374:Neoplasms, Experimental / Q000188:drug therapy*
chemical_list: D000970:Antineoplastic Agents; D004304:Dosage Forms; D008081:Liposomes; D002945:Cisplatin
keywords: Antitumor activity; Cisplatin; Liposomes; Oleic acid; Pharmacokinetics