title: Capsular polysaccharide of Group B Streptococcus mediates biofilm formation in the presence of human plasma.
abstract: Group B Streptococcus (GBS) is an asymptomatic colonizer of human mucosal surfaces that is responsible for sepsis and meningitis in neonates. Bacterial persistence and pathogenesis often involves biofilm formation. We previously showed that biofilm formation in medium supplemented with glucose is mediated by the PI-2a pilus. Here, biofilm formation was tested in cell culture medium supplemented with human plasma. GBS strains were able to form biofilms in these conditions unlike Group A Streptococcus (GAS) or Staphylococcus aureus. Analysis of mutants impaired for various surface components revealed that the GBS capsule is a key component in this process.
authors.affiliation: Institut Pasteur, Unité des Bactéries Pathogènes à Gram positif, 75015 Paris, France; Centre National de la Recherche Scientifique (CNRS ERL 3526), France.
authors.forename: Fan Di
authors.initials: FD
authors.lastname: Xia
authors.affiliation: Institut Pasteur, Imagopole, 75015 Paris, France.
authors.forename: Adeline
authors.initials: A
authors.lastname: Mallet
authors.affiliation: Institut Pasteur, Unité des Bactéries Pathogènes à Gram positif, 75015 Paris, France; Centre National de la Recherche Scientifique (CNRS ERL 3526), France.
authors.forename: Elise
authors.initials: E
authors.lastname: Caliot
authors.affiliation: Institut Pasteur, Unité des Bactéries Pathogènes à Gram positif, 75015 Paris, France; Centre National de la Recherche Scientifique (CNRS ERL 3526), France.
authors.forename: Cherry
authors.initials: C
authors.lastname: Gao
authors.affiliation: Institut Pasteur, Unité des Bactéries Pathogènes à Gram positif, 75015 Paris, France; Centre National de la Recherche Scientifique (CNRS ERL 3526), France.
authors.forename: Patrick
authors.initials: P
authors.lastname: Trieu-Cuot
authors.affiliation: Institut Pasteur, Unité des Bactéries Pathogènes à Gram positif, 75015 Paris, France; Centre National de la Recherche Scientifique (CNRS ERL 3526), France. Electronic address: shaynoor.dramsi@pasteur.fr.
authors.forename: Shaynoor
authors.initials: S
authors.lastname: Dramsi
mesh_terms: D018441:Biofilms / Q000254:growth & development*; D003470:Culture Media / Q000737:chemistry; D006801:Humans; D010949:Plasma / Q000382:microbiology*; D011135:Polysaccharides, Bacterial / Q000378:metabolism*; D013211:Staphylococcus aureus / Q000254:growth & development / Q000502:physiology; D013292:Streptococcus agalactiae / Q000254:growth & development / Q000502:physiology*; D013297:Streptococcus pyogenes / Q000254:growth & development / Q000502:physiology
chemical_list: D003470:Culture Media; D011135:Polysaccharides, Bacterial
keywords: Biofilm; Capsular polysaccharide; GBS; Human plasma; Streptococcus