title: [Bacillus subtilis ypaA gene regulation mechanism involves FMN-binding sensor RNA].
abstract: We studied the regulation of the Bacillus subtilis ypaA gene-encoding riboflavin-transporter protein involving FMN-dependent sensor RNA. Using translational fusions of the wild-type ypaA gene with the lacZ-reporter gene in the leader region we showed that in vivo ypaA gene expression decreased more than 10-fold in the presence of endogenous FMN. Introduction of two nucleotide substitutions providing stabilization of the sequester hairpins results in almost complete repression of reporter gene expression. Using toeprint assay in vitro it has been shown that FMN presence inhibits the formation of the 30S initiation complexint the ypaA gene leader mRNA. Our results support the model of ypaA gene regulation whereby FMN binding with the ypaA gene leader sequence results in translation suppression through the sequestering of the SD-sequence.
authors.forename: S A
authors.initials: SA
authors.lastname: Sklyarova
authors.forename: A S
authors.initials: AS
authors.lastname: Mironov
mesh_terms: D001412:Bacillus subtilis / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D005486:Flavin Mononucleotide / Q000494:pharmacology*; D026901:Membrane Transport Proteins / Q000235:genetics* / Q000378:metabolism; D010442:Peptide Chain Initiation, Translational; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D026901:Membrane Transport Proteins; D012333:RNA, Messenger; D005486:Flavin Mononucleotide