title: Growth of oleaginous Rhodotorula glutinis in an internal-loop airlift bioreactor by using lignocellulosic biomass hydrolysate as the carbon source.
abstract: The conversion of abundant lignocellulosic biomass (LCB) to valuable compounds has become a very attractive idea recently. This study successfully used LCB (rice straw) hydrolysate as a carbon source for the cultivation of oleaginous yeast-Rhodotorula glutinis in an airlift bioreactor. The lipid content of 34.3 ± 0.6% was obtained in an airlift batch with 60 g reducing sugars/L of LCB hydrolysate at a 2 vvm aeration rate. While using LCB hydrolysate as the carbon source, oleic acid (C18:1) and linoleic acid (C18:2) were the predominant fatty acids of the microbial lipids. Using LCB hydrolysate in the airlift bioreactor at 2 vvm achieved the highest cell mass growth as compared to the agitation tank. Despite the low lipid content of the batch using LCB hydrolysate, this low cost feedstock has the potential of being adopted for the production of β-carotene instead of lipid accumulation in the airlift bioreactor for the cultivation of R. glutinis.
authors.affiliation: Department of Chemical and Materials Engineering, Tunghai University, Taiwan, ROC. Electronic address: hwyen@thu.edu.tw.
authors.forename: Hong-Wei
authors.initials: HW
authors.lastname: Yen
authors.affiliation: Department of Chemical and Materials Engineering, Tunghai University, Taiwan, ROC.
authors.forename: Jung-Tzu
authors.initials: JT
authors.lastname: Chang
mesh_terms: D018533:Biomass; D019149:Bioreactors / Q000382:microbiology*; D002244:Carbon / Q000378:metabolism*; D008031:Lignin / Q000737:chemistry* / Q000378:metabolism*; D019787:Linoleic Acid / Q000032:analysis / Q000737:chemistry; D019301:Oleic Acid / Q000032:analysis / Q000737:chemistry; D012275:Oryza / Q000737:chemistry / Q000378:metabolism; D012248:Rhodotorula / Q000254:growth & development* / Q000378:metabolism*; D019207:beta Carotene / Q000096:biosynthesis
chemical_list: D019207:beta Carotene; C036909:lignocellulose; D019301:Oleic Acid; D002244:Carbon; D008031:Lignin; D019787:Linoleic Acid
keywords: Airlift bioreactor; Hydrolysate; Oleaginous; Rhodotorula; Rice straw