title: Comparison of human olfactory and skeletal MSCs using osteogenic nanotopography to demonstrate bone-specific bioactivity of the surfaces.
abstract: Recently we identified a novel population of mesenchymal stem cells (MSCs) from human olfactory mucosa (OM-MSCs), a tissue which promotes neurogenesis throughout life, and demonstrated that they promoted CNS myelination to a greater extent than bone marrow-derived (BM)-MSCs. Previous data demonstrated that nanotopographies with a degree of disorder induce BM-MSC osteogenic differentiation. Thus, using biomaterials as non-chemical tools, we investigated if MSCs from a completely different cellular niche could be induced to differentiate similarly to nanoscale cues alone. Both MSCs differentiated into bone when cultured on nanotopographically embossed polycaprolactone (PCL) with a disordered pattern and heights but not on a "smooth" non-embossed PCL control substrate, but OM-MSC changes were at lower expression levels. Both MSCs showed similar increases in differentiation markers at the protein and mRNA level when plated on the two patterned surfaces. Thus, topographical cues from substrates with disordered patterns can up-regulate several MSC resident genes in both BM-MSCs and OM-MSCs. Moreover, antibody purified BM-MSCs had similar properties to non-purified BM-MSCs. These data suggest that MSCs from a neural cellular niche express similar bone-induced cues to BM-MSCs, suggesting that MSCs that inherently support nervous tissue can differentiate along the bone lineage in a similar manner to MSCs from a skeletal environment.
authors.affiliation: Institute of Infection, Immunity and Inflammation, College of Medical, Veterinary and Life Sciences, University of Glasgow, 120 University Place, Glasgow G12 8TA, UK.
authors.forename: Steven A
authors.initials: SA
authors.lastname: Johnstone
authors.affiliation: Swiss Centre for Electronics and Microtechnology, CSEM SA, Nanotechnology and Life Sciences, Jaquet-Droz 1, 2002 4, Switzerland.
authors.forename: Martha
authors.initials: M
authors.lastname: Liley
authors.affiliation: Centre for Cell Engineering, Institute of Molecular, Cell and Systems Biology, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow G12 8QQ, UK.
authors.forename: Matthew J
authors.initials: MJ
authors.lastname: Dalby
authors.affiliation: Institute of Infection, Immunity and Inflammation, College of Medical, Veterinary and Life Sciences, University of Glasgow, 120 University Place, Glasgow G12 8TA, UK. Electronic address: susan.barnett@glasgow.ac.uk.
authors.forename: Susan C
authors.initials: SC
authors.lastname: Barnett
mesh_terms: D000328:Adult; D001842:Bone and Bones / Q000166:cytology / Q000378:metabolism*; D002478:Cells, Cultured; D060833:Cellular Microenvironment; D005260:Female; D006801:Humans; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism*; D008875:Middle Aged; D009831:Olfactory Mucosa / Q000166:cytology / Q000378:metabolism*; D010012:Osteogenesis; D011091:Polyesters / Q000737:chemistry; D013499:Surface Properties
chemical_list: D011091:Polyesters; C016240:polycaprolactone
keywords: Bone; Mesenchymal stem cells; Nanotopographies; Olfactory mucosa