title: Means to optimize protein expression in transgenic plants.
abstract: The biotechnological production of proteins is currently achieved via expression systems derived from different lineages. In the past years transgenic plants have proven to be able to compete with bacteria or mammalian cell systems. Gene engineering approaches exist to raise yields by controlling mandatory processes in the course of biopharmaceutical protein production. Here we review and discuss the current status and recent improvements of parameters influencing recombinant protein production in transgenic plants. In particular, this review focuses on the so-called inside (mRNA sequence and structure) and outside factors (host and production system/conditions), which are adjustable and allow to optimize protein production via gene engineering.
authors.affiliation: Plant Cell Biology, Faculty of Biology, University of Marburg, Karl-von-Frisch-Str. 8, D-35043 Marburg, Germany.
authors.forename: Kristian K
authors.initials: KK
authors.lastname: Ullrich
authors.affiliation: Plant Cell Biology, Faculty of Biology, University of Marburg, Karl-von-Frisch-Str. 8, D-35043 Marburg, Germany.
authors.forename: Manuel
authors.initials: M
authors.lastname: Hiss
authors.affiliation: Plant Cell Biology, Faculty of Biology, University of Marburg, Karl-von-Frisch-Str. 8, D-35043 Marburg, Germany; BIOSS Centre for Biological Signalling Studies, University of Freiburg, Germany. Electronic address: stefan.rensing@biologie.uni-marburg.de.
authors.forename: Stefan A
authors.initials: SA
authors.lastname: Rensing
mesh_terms: D015870:Gene Expression; D006801:Humans; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics; D018749:RNA, Plant / Q000235:genetics; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D010940:Plant Proteins; D012333:RNA, Messenger; D018749:RNA, Plant; D011994:Recombinant Proteins