title: In vitro antifilarial activity of Azadirachta indica aqueous extract through reactive oxygen species enhancement.
abstract: OBJECTIVE
  To evaluate an aqueous preparation from the Azadirachta indica leaves (AEA) against Setaria cervi (S. cervi), a model filarial parasite.
  
  
  METHODS
  In vitro efficacy of AEA was evaluated against S. cervi through estimation of relative motility value, dye exclusion test and MTT assay. Visible morphological alterations were monitored using conventional microscopic techniques in microfilariae and haematoxylin-eosin stained sections of AEA-treated adults.
  
  
  RESULTS
  Enhancement of reactive oxygen species in S. cervi treated with AEA was established through alteration in the activity of glutathione S-transferase, superoxide dismutase, catalase, peroxidase and level of superoxide anion and reduced glutathione.
  
  
  CONCLUSIONS
  In vitro filaricidal activity of AEA is possibly through disturbing redox homeostasis by down-regulating and altering the level of some key antioxidants and regulatory enzymes like reduced glutathione, glutathione S-transferase, superoxide dismutase, catalase and glutathione peroxidase of S. cervi.
authors.affiliation: Parasitology Laboratory, Department of Zoology (Centre for Advanced Studies), Visva-Bharati University, Santiniketan-731 235, West Bengal, India.
authors.forename: Niladri
authors.initials: N
authors.lastname: Mukherjee
authors.affiliation: Parasitology Laboratory, Department of Zoology (Centre for Advanced Studies), Visva-Bharati University, Santiniketan-731 235, West Bengal, India.
authors.forename: Prasanta
authors.initials: P
authors.lastname: Saini
authors.affiliation: Parasitology Laboratory, Department of Zoology (Centre for Advanced Studies), Visva-Bharati University, Santiniketan-731 235, West Bengal, India.
authors.forename: Suprabhat
authors.initials: S
authors.lastname: Mukherjee
authors.affiliation: Parasitology Laboratory, Department of Zoology (Centre for Advanced Studies), Visva-Bharati University, Santiniketan-731 235, West Bengal, India.
authors.forename: Priya
authors.initials: P
authors.lastname: Roy
authors.affiliation: Parasitology Laboratory, Department of Zoology (Centre for Advanced Studies), Visva-Bharati University, Santiniketan-731 235, West Bengal, India. Electronic address: spsinhababu@gmail.com.
authors.forename: Santi P
authors.initials: SP
authors.lastname: Sinha Babu
keywords: Antifilarial; Azadirachta indica; Reactive oxygen species; Setaria cervi