title: Insulin-Like growth factor 1 related pathways and high-fat diet promotion of transgenic adenocarcinoma mouse prostate (TRAMP) cancer progression.
abstract: BACKGROUND
  We aimed to investigate the role of IGF-1 related pathway in high-fat diet (HFD) promotion of TRAMP mouse PCa progression.
  
  
  METHODS
  TRAMP mice were randomly divided into two groups: HFD group and normal diet group. TRAMP mice of both groups were sacrificed and sampled on the 20th, 24th and 28th week respectively. Serum levels of insulin, IGF-1 and IGF-2 were tested by ELISA. Prostate tissue of TRAMP mice was used for both HE staining and immunohistochemical staining of IGF-1 related pathway proteins, including IGF-1Rα, IGF -1Rβ, IGFBPs and AKT.
  
  
  RESULTS
  The mortality of TRAMP mice from HFD group was significantly higher than that of normal diet group (23.81% and 7.14%, p=.035). The tumor incidence of HFD TRAMP mice at 20(th) week was significantly higher than normal diet group (78.57% and 35.71%, p=.022). Serum IGF-1 level of HFD TRAMP mice was significantly higher than that of normal diet TRAMP mice. Serum IGF-1 level tended to increase with HFD TRAMP mice's age. HFD TRAMP mice had higher positive staining rate of IGF-1Rα, IGF-1Rβ, IGFBP3 and Akt than normal diet TRAMP mice.
  
  
  CONCLUSIONS
  IGF-1 related pathway played an important role in high-fat diet promotion of TRAMP mouse PCa development and progression.
authors.affiliation: Departamento de Urología, Hospital Huashan, Universidad Fudan, Shanghai, PR China.
authors.forename: H
authors.initials: H
authors.lastname: Xu
authors.affiliation: Departamento de Urología, Hospital Huashan, Universidad Fudan, Shanghai, PR China. Electronic address: haowen_jiang@126.com.
authors.forename: H W
authors.initials: HW
authors.lastname: Jiang
authors.affiliation: Departamento de Urología, Hospital Huashan, Universidad Fudan, Shanghai, PR China.
authors.forename: Q
authors.initials: Q
authors.lastname: Ding
mesh_terms: D000230:Adenocarcinoma / Q000209:etiology / Q000473:pathology*; D000818:Animals; D059305:Diet, High-Fat / Q000009:adverse effects*; D018450:Disease Progression; D018972:Insulin-Like Growth Factor Binding Protein 3 / Q000032:analysis; D007334:Insulin-Like Growth Factor I / Q000032:analysis / Q000502:physiology*; D007335:Insulin-Like Growth Factor II / Q000032:analysis; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D009363:Neoplasm Proteins / Q000097:blood; D011471:Prostatic Neoplasms / Q000209:etiology / Q000473:pathology*; D051057:Proto-Oncogene Proteins c-akt / Q000097:blood; D011897:Random Allocation; D017526:Receptor, IGF Type 1 / Q000097:blood; D015398:Signal Transduction
chemical_list: C513121:IGF2 protein, mouse; D018972:Insulin-Like Growth Factor Binding Protein 3; D009363:Neoplasm Proteins; C519530:insulin-like growth factor-1, mouse; D007334:Insulin-Like Growth Factor I; D007335:Insulin-Like Growth Factor II; D017526:Receptor, IGF Type 1; C514862:Akt1 protein, mouse; D051057:Proto-Oncogene Proteins c-akt
keywords: Cáncer de próstata; Dieta alta en grasa; Factor de crecimiento tipo insulina; High-fat diet; Insulin like growth factor; Prostate cancer; TRAMP