title: Circulating CD133+ CD34+ progenitor cells and plasma stromal-derived factor-1alpha: predictive role in ischemic stroke patients.
abstract: Circulating progenitor cells and stromal-derived factor-1alpha (SDF-1α) have been suggested to participate in tissue repair after ischemic injury. However, the predictive role of circulating CD133+ CD34+ progenitors and plasma SDF-1α in ischemic stroke (IS) patients remains unknown. In this study, we recruited 95 acute IS patients, 40 at-risk subjects, and 30 normal subjects. The National Institutes of Health Stroke Scale (NIHSS), infarct volume, and carotid intima-media thickness (IMT) were determined at day 1 and the modified Rankin scale (mRS) of functional outcome was assessed at day 21. The levels of circulating CD133+ CD34+ cells and plasma SDF-1α were determined by flow cytometry and enzyme-linked immunosorbent assay, respectively. Our data showed that: (1) the levels of CD133+ CD34+ cells were lower in at-risk subjects and IS patients at admission (day 1) when compared with normal controls; (2) the day 1 level of CD133+ CD34+ cells varied in IS subgroups and inversely correlated with NIHSS and carotid IMT and the level of SDF-1α inversely correlated with NIHSS and infarct volume; (3) the increment rates of circulating CD133+ CD34+ cells and plasma SDF-1α within the first week were correlated; and (4) patients with a higher level of CD133+ CD34+ cells at day 7 had a low mRS. The increased rate of CD133+ CD34+ cells in the first week was inversely associated with mRS. In conclusion, our findings demonstrate that the circulating CD133+ CD34+ progenitor cells and plasma SDF-1α can be used as predictive parameters for IS severity and outcome.
authors.affiliation: Clinical Research Center and Department of Neurology, The Affiliated Hospital of Guangdong Medical College, Zhanjiang, Guangdong, China.
authors.forename: Yusen
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Clinical Research Center and Department of Neurology, The Affiliated Hospital of Guangdong Medical College, Zhanjiang, Guangdong, China.
authors.forename: Bing
authors.initials: B
authors.lastname: Lu
authors.affiliation: Department of Pharmacology & Toxicology, Boonshoft School of Medicine, Wright State University, Dayton, Ohio.
authors.forename: Jinju
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Pharmacology & Toxicology, Boonshoft School of Medicine, Wright State University, Dayton, Ohio.
authors.forename: Shuzhen
authors.initials: S
authors.lastname: Chen
authors.affiliation: Clinical Research Center and Department of Neurology, The Affiliated Hospital of Guangdong Medical College, Zhanjiang, Guangdong, China.
authors.forename: Zhijun
authors.initials: Z
authors.lastname: Lin
authors.affiliation: Clinical Research Center and Department of Neurology, The Affiliated Hospital of Guangdong Medical College, Zhanjiang, Guangdong, China.
authors.forename: Xiaotang
authors.initials: X
authors.lastname: Ma
authors.affiliation: Clinical Research Center and Department of Neurology, The Affiliated Hospital of Guangdong Medical College, Zhanjiang, Guangdong, China.
authors.forename: Yajing
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Clinical Research Center and Department of Neurology, The Affiliated Hospital of Guangdong Medical College, Zhanjiang, Guangdong, China. Electronic address: binzhaoe@163.com.
authors.forename: Bin
authors.initials: B
authors.lastname: Zhao
authors.affiliation: Clinical Research Center and Department of Neurology, The Affiliated Hospital of Guangdong Medical College, Zhanjiang, Guangdong, China; Department of Pharmacology & Toxicology, Boonshoft School of Medicine, Wright State University, Dayton, Ohio. Electronic address: yanfang.chen@wright.edu.
authors.forename: Yanfang
authors.initials: Y
authors.lastname: Chen
mesh_terms: D000071916:AC133 Antigen; D000368:Aged; D015703:Antigens, CD / Q000378:metabolism*; D018952:Antigens, CD34 / Q000378:metabolism*; D002545:Brain Ischemia / Q000097:blood / Q000175:diagnosis* / Q000473:pathology; D054377:Chemokine CXCL12 / Q000097:blood*; D005260:Female; D006023:Glycoproteins / Q000378:metabolism*; D006801:Humans; D008297:Male; D010455:Peptides / Q000378:metabolism*; D011237:Predictive Value of Tests; D011379:Prognosis; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism; D020521:Stroke / Q000097:blood / Q000175:diagnosis* / Q000473:pathology
chemical_list: D000071916:AC133 Antigen; D015703:Antigens, CD; D018952:Antigens, CD34; D054377:Chemokine CXCL12; D006023:Glycoproteins; C000606023:PROM1 protein, human; D010455:Peptides
keywords: CD133+ CD34+ cells; Progenitor cell; SDF-1α; ischemic stroke