title: Experimental study on the optimization of extraction process of garlic oil and its antibacterial effects.
abstract: BACKGROUND
  Garlic oil which is the main active constituent of garlic has a wide range of pharmacological activities, and a broad antibacterial spectrum. It also has a strong anti-cancer activity, and can significantly inhibit a variety of tumors such as liver cancer, gastric cancer and colon cancer. The objective is to study the extraction process of garlic oil and its antibacterial effects.
  
  
  METHODS
  CO2 Supercritical extraction was used to investigate the optimal processing conditions for garlic oil extraction; filter paper test and suspension dilution test were applied to determine the bacteriostatic action of garlic oil.
  
  
  RESULTS
  In the CO2 supercritical extraction experiment, factors influencing the yield of garlic oil were: extraction pressure > extraction temperature > extraction time in descending order. Range analysis showed that the optimal experimental conditions for CO2 supercritical extraction of garlic oil were extraction pressure of 15 Mpa, temperature of 40 °C, and duration of 1 h. Different concentrations of garlic oil could all inhibit the growth of Staphylococcus aureus, Escherichia coli and Bacillus subtilis, suggesting that garlic oil has an antibacterial effect.
  
  
  CONCLUSIONS
  The optimal experimental conditions for CO2 supercritical extraction of garlic oil were: extraction pressure of 15 Mpa, temperature of 40 °C, and duration of 1 h; garlic oil has an antibacterial effect.
authors.affiliation: College of Materials and Chemistry and Chemical Engineering, Chengdu University of Technology, Chengdu, Sichuan 610059, China.
authors.forename: Yajie
authors.initials: Y
authors.lastname: Guo
mesh_terms: D000498:Allyl Compounds / Q000302:isolation & purification* / Q000494:pharmacology*; D000900:Anti-Bacterial Agents / Q000302:isolation & purification* / Q000494:pharmacology*; D001412:Bacillus subtilis / Q000187:drug effects / Q000254:growth & development; D025924:Chromatography, Supercritical Fluid / Q000379:methods*; D004926:Escherichia coli / Q000187:drug effects / Q000254:growth & development; D005737:Garlic / Q000737:chemistry*; D013211:Staphylococcus aureus / Q000187:drug effects / Q000254:growth & development; D013440:Sulfides / Q000302:isolation & purification* / Q000494:pharmacology*
chemical_list: D000498:Allyl Compounds; D000900:Anti-Bacterial Agents; D013440:Sulfides; C038491:allyl sulfide
keywords: Bacillus subtilis; CO2; Escherichia coli; Staphylococcus aureus; garlic oil; supercritical extraction