title: The effect of numbers of frozen-thawed boar sperm and addition of prostaglandin F2α at insemination on fertility in pigs.
abstract: Frozen-thawed boar sperm (FTS) has reduced fertility compared to liquid semen. Exogenous prostaglandin administered at insemination has been reported to improve cases of low fertility. This experiment tested the effect of number of FTS and addition of prostaglandin (PGF2α) on fertility. The experiment was performed in replicates using weaned sows (n=24) and synchronized gilts (n=94). All females were induced into estrus using PG600® at weaning or following estrus synchronization. At estrus, females received 0.5, 1.0, or 2 billion motile FTS (n=9 boars) with 0 or 5mg of PGF2α added into each AI dose at insemination. Inseminations occurred at 24 and 36h after onset of estrus and ovulation was monitored by ultrasound. Pregnancy and litter size were determined for sows at farrowing and d 50 of gestation for gilts at slaughter. There was no effect of PGF2α and no interaction with dose of FTS or parity on fertility (P>0.10). Pregnancy rate was affected by FTS dose (P<0.001) with 2.0×10(9) (76.3%) greater than 0.5×10(9) (46.2%) and 1.0×10(9) sperm (48.8±8.0%). Pregnancy rate was not affected by parity (P>0.10) but was influenced by boar (P<0.05). Number of fetuses was also affected by FTS dose (P<0.001) with 2.0×10(9) (10.1) and 1.0×10(9) (9.4) producing more pigs than 0.5×10(9) sperm (6.9±0.9). Litter size was also affected by parity (P=0.001) and boar (P<0.01). These results indicate that AI using 2.0×10(9) FTS can result in acceptable pregnancy rates and litter sizes but with no measurable benefit for addition of prostaglandin.
authors.affiliation: Department of Animal Sciences, University of Illinois, 1207 West Gregory Avenue, Urbana, IL, USA 61801. Electronic address: rknox@illinois.edu.
authors.forename: Robert V
authors.initials: RV
authors.lastname: Knox
authors.affiliation: Department of Animal Sciences, University of Illinois, 1207 West Gregory Avenue, Urbana, IL, USA 61801.
authors.forename: Brandon M
authors.initials: BM
authors.lastname: Yantis
mesh_terms: D000818:Animals; D015237:Dinoprost / Q000494:pharmacology*; D005260:Female; D005298:Fertility / Q000187:drug effects; D005615:Freezing; D007315:Insemination, Artificial / Q000662:veterinary; D008297:Male; D011247:Pregnancy; D018873:Pregnancy Rate; D012661:Semen / Q000166:cytology / Q000187:drug effects*; D012662:Semen Preservation / Q000662:veterinary; D013076:Sperm Count / Q000662:veterinary; D013081:Sperm Motility / Q000187:drug effects; D013552:Swine
chemical_list: D015237:Dinoprost
keywords: Artificial insemination; Fertility; Frozen boar sperm; Litter size; Prostaglandin; Swine