title: Improvement in the DNA sequencing of genomes bearing long  repeated elements.
abstract: When using next-generation sequencing methods, obtaining a single and complete bacterial chromosomic sequence may be challenging especially for bacteria harboring multiple repeated sequences. Here we present a one-step procedure to optimize the sequencing process in order to obtain a single chromosomic scaffold.
authors.affiliation: Institut de Biologie Intégrative et des Systèmes (IBIS), Université Laval, Quebec City, QC G1V 0A6, Canada.
authors.forename: Antony T
authors.initials: AT
authors.lastname: Vincent
authors.forename: Brian
authors.initials: B
authors.lastname: Boyle
authors.forename: Nicolas
authors.initials: N
authors.lastname: Derome
authors.forename: Steve J
authors.initials: SJ
authors.lastname: Charette
mesh_terms: D002876:Chromosomes, Bacterial; D019295:Computational Biology / Q000379:methods; D016680:Genome, Bacterial; D023281:Genomics / Q000379:methods; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods / Q000592:standards; D012091:Repetitive Sequences, Nucleic Acid