title: Inhibitive effect of IL-24 gene on CD133(+) laryngeal cancer cells.
abstract: OBJECTIVE
  To explore the inhibitive and apoptosis inductive effect of IL-24 genes on CD133(+) laryngeal cancer cells in Hep-2 line.
  
  
  METHODS
  Human peripheral blood monocytes were isolated. The total RNA was extracted by using Trizol method and reverse transcripted into cDNA using RT-PCR method. Primers P1 and P2 was designed for the amplification of human IL-24 genes. After confirmation of agarose gel electrophoresis tests, TA was cloned into pMD19-T simple vector. Nhe I and Xho I double digesting human IL-24 and pIRES2-ZsGreen1 and eukaryotic expression vector were used to establish the pIRES2-ZsGreen1-hIL-24 vector, and detected by enzyme digestion and gene sequencing methods. Flow cytometry (FCM) was used to isolate CD133(+) cells from Hep-2 cells. CD133(+) cells were transfected with pIRES2-ZsGreen1-hIL-24 through liposome 2000. After detection, MTT and FCM were used to observe the effect of IL-24 gene on CD133(+) laryngeal cancer Hep-2 cells.
  
  
  RESULTS
  Lipotin mediated transfection of recombinant pIRES2-ZsGreen1-hIL-24 plasmid into CD133(+) Hep-2 could expressed IL-24 gene in cells stably. MTT results showed that IL-24 transfected group was significantly suppressed compared to empty vector group and control group (P<0.05); FCM results showed that the apoptosis rate of experimental group increased significantly compared to empty vector group and control group (P<0.05).
  
  
  CONCLUSIONS
  IL-24 gene expressions can inhibit proliferation of CD133(+) laryngeal cells in Hep-2 line and promote their apoptosis.
authors.affiliation: Second Affiliated Hospital of Jilin University, 130041, Jilin, China.
authors.forename: Jin-Zhang
authors.initials: JZ
authors.lastname: Cheng
authors.affiliation: Second Affiliated Hospital of Jilin University, 130041, Jilin, China. Electronic address: jinchunshun@163.com.
authors.forename: Dan
authors.initials: D
authors.lastname: Yu
authors.affiliation: Second Affiliated Hospital of Jilin University, 130041, Jilin, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Second Affiliated Hospital of Jilin University, 130041, Jilin, China. Electronic address: yudan19792003@163.com.
authors.forename: Chun-Shun
authors.initials: CS
authors.lastname: Jin
authors.affiliation: Second Affiliated Hospital of Jilin University, 130041, Jilin, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Second Affiliated Hospital of Jilin University, 130041, Jilin, China.
authors.forename: Xue
authors.initials: X
authors.lastname: Zhao
authors.affiliation: Second Affiliated Hospital of Jilin University, 130041, Jilin, China.
authors.forename: Xin-Meng
authors.initials: XM
authors.lastname: Qi
authors.affiliation: Second Affiliated Hospital of Jilin University, 130041, Jilin, China.
authors.forename: Xueshi-Bojie
authors.initials: XB
authors.lastname: Liu
keywords: CD133; Cancer stem cells; Gene therapy; IL-24; Laryngeal cancer