title: Isolation and identification of cyclic lipopeptides from Paenibacillus ehimensis, strain IB-X-b.
abstract: Antifungal lipopeptides produced by an antagonistic bacterium, Paenibacillus ehimensis strain IB-X-b, were purified and analyzed. The acetone extract of the culture supernatant contained an antifungal amphiphilic fraction stainable with ninhydrin on thin layer chromatography. The fraction was further purified with water-methanol extraction followed by a chromatography on a C18-support. The analysis with LC-MS showed presence of two main series of homologous compounds, family of depsipeptides containing a hydroxy fatty acid, three 2,4-diaminobutyric acid (Dab) residues, five hydrophobic amino acids and one Ser/Thr residue, and cyclic lipopeptides of bacillomycin L and fengycin/plipastatin/agrastatin families. The prevailing compounds in this group are bacillomycin L-C15, fengycin/plipastatin A-C16together with their homologues responsible for the majority of fungal growth inhibition by P. ehimensis IB-X-b.
authors.affiliation: Institute of Biology, Ufa Research Centre of Russian Academy of Sciences, 450054 Ufa, Russia. Electronic address: gleakt@anrb.ru.
authors.forename: Gleb
authors.initials: G
authors.lastname: Aktuganov
authors.affiliation: Division of Microbiology, Department of Food and Environmental Sciences, University of Helsinki, Viikki Biocenter, P.O. Box 56, 00014 Helsinki, Finland.
authors.forename: Jouni
authors.initials: J
authors.lastname: Jokela
authors.affiliation: Department of Chemistry, University of Turku, 20014 Turku, Finland.
authors.forename: Henri
authors.initials: H
authors.lastname: Kivelä
authors.affiliation: Technology Research Centre, University of Turku, 20520 Turku, Finland.
authors.forename: Elvira
authors.initials: E
authors.lastname: Khalikova
authors.affiliation: Institute of Biology, Ufa Research Centre of Russian Academy of Sciences, 450054 Ufa, Russia.
authors.forename: Alexander
authors.initials: A
authors.lastname: Melentjev
authors.affiliation: Institute of Biology, Ufa Research Centre of Russian Academy of Sciences, 450054 Ufa, Russia.
authors.forename: Nailia
authors.initials: N
authors.lastname: Galimzianova
authors.affiliation: Institute of Biology, Ufa Research Centre of Russian Academy of Sciences, 450054 Ufa, Russia.
authors.forename: Lyudmila
authors.initials: L
authors.lastname: Kuzmina
authors.affiliation: Turku Centre for Biotechnology, University of Turku and Ǻbo Academi, 20520 Turku, Finland. Electronic address: petri.kouvonen@btk.fi.
authors.forename: Petri
authors.initials: P
authors.lastname: Kouvonen
authors.affiliation: Structural Biology Program, Sloan-Kettering Institute for Cancer Research, 10021 NY, United States.
authors.forename: Juha-Pekka
authors.initials: JP
authors.lastname: Himanen
authors.affiliation: Department of Virology, Institute of Microbiology and Pathology, University of Turku, 20520 Turku, Finland.
authors.forename: Petri
authors.initials: P
authors.lastname: Susi
authors.affiliation: Technology Research Centre, University of Turku, 20520 Turku, Finland.
authors.forename: Timo
authors.initials: T
authors.lastname: Korpela
keywords: Antifungal compounds; Biocontrol; Cyclic lipopeptides; LC–MS; Microbial antagonism; Paenibacillus ehimensis; Purification