title: Native lysozyme and dry-heated lysozyme interactions with membrane lipid monolayers: lateral reorganization of LPS monolayer, model of the Escherichia coli outer membrane.
abstract: Lysozyme is mainly described active against Gram-positive bacteria, but is also efficient against some Gram-negative species. Especially, it was recently demonstrated that lysozyme disrupts Escherichia coli membranes. Moreover, dry-heating changes the physicochemical properties of the protein and increases the membrane activity of lysozyme. In order to elucidate the mode of insertion of lysozyme into the bacterial membrane, the interaction between lysozyme and a LPS monolayer mimicking the E. coli outer membrane has been investigated by tensiometry, ellipsometry, Brewster angle microscopy and atomic force microscopy. It was thus established that lysozyme has a high affinity for the LPS monolayer, and is able to insert into the latter as long as polysaccharide moieties are present, causing reorganization of the LPS monolayer. Dry-heating increases the lysozyme affinity for the LPS monolayer and its insertion capacity; the resulting reorganization of the LPS monolayer is different and more drastic than with the native protein.
authors.affiliation: Agrocampus Ouest, UMR1253 Science et Technologie du Lait et de l'Oeuf, F-35042 Rennes, France; INRA, UMR1253 Science et Technologie du Lait et de l'Oeuf, F-35042 Rennes, France. Electronic address: melanie.derde@agrocampus-ouest.fr.
authors.forename: Melanie
authors.initials: M
authors.lastname: Derde
authors.affiliation: Agrocampus Ouest, UMR1253 Science et Technologie du Lait et de l'Oeuf, F-35042 Rennes, France; INRA, UMR1253 Science et Technologie du Lait et de l'Oeuf, F-35042 Rennes, France.
authors.forename: Françoise
authors.initials: F
authors.lastname: Nau
authors.affiliation: Agrocampus Ouest, UMR1253 Science et Technologie du Lait et de l'Oeuf, F-35042 Rennes, France; INRA, UMR1253 Science et Technologie du Lait et de l'Oeuf, F-35042 Rennes, France.
authors.forename: Valérie
authors.initials: V
authors.lastname: Lechevalier
authors.affiliation: Agrocampus Ouest, UMR1253 Science et Technologie du Lait et de l'Oeuf, F-35042 Rennes, France; INRA, UMR1253 Science et Technologie du Lait et de l'Oeuf, F-35042 Rennes, France.
authors.forename: Catherine
authors.initials: C
authors.lastname: Guérin-Dubiard
authors.affiliation: Université de Rennes 1, Institut de Physique de Rennes, UMR6251, CNRS, F-35042 Rennes, France.
authors.forename: Gilles
authors.initials: G
authors.lastname: Paboeuf
authors.affiliation: Agrocampus Ouest, UMR1253 Science et Technologie du Lait et de l'Oeuf, F-35042 Rennes, France; INRA, UMR1253 Science et Technologie du Lait et de l'Oeuf, F-35042 Rennes, France.
authors.forename: Sophie
authors.initials: S
authors.lastname: Jan
authors.affiliation: Agrocampus Ouest, UMR1253 Science et Technologie du Lait et de l'Oeuf, F-35042 Rennes, France; INRA, UMR1253 Science et Technologie du Lait et de l'Oeuf, F-35042 Rennes, France.
authors.forename: Florence
authors.initials: F
authors.lastname: Baron
authors.affiliation: Agrocampus Ouest, UMR1253 Science et Technologie du Lait et de l'Oeuf, F-35042 Rennes, France; INRA, UMR1253 Science et Technologie du Lait et de l'Oeuf, F-35042 Rennes, France.
authors.forename: Michel
authors.initials: M
authors.lastname: Gautier
authors.affiliation: Université de Rennes 1, Institut de Physique de Rennes, UMR6251, CNRS, F-35042 Rennes, France. Electronic address: veronique.vie@univ-rennes1.fr.
authors.forename: Véronique
authors.initials: V
authors.lastname: Vié
mesh_terms: D000465:Algorithms; D001667:Binding, Competitive; D002462:Cell Membrane / Q000737:chemistry / Q000378:metabolism; D003890:Desiccation; D004926:Escherichia coli / Q000737:chemistry / Q000378:metabolism; D006358:Hot Temperature; D016014:Linear Models; D008070:Lipopolysaccharides / Q000737:chemistry / Q000378:metabolism; D008563:Membrane Lipids / Q000737:chemistry* / Q000378:metabolism; D008853:Microscopy; D018625:Microscopy, Atomic Force; D008954:Models, Biological; D015394:Molecular Structure; D009113:Muramidase / Q000737:chemistry* / Q000378:metabolism; D011485:Protein Binding; D013816:Thermodynamics; D053835:Unilamellar Liposomes / Q000737:chemistry* / Q000378:metabolism
chemical_list: D008070:Lipopolysaccharides; D008563:Membrane Lipids; D053835:Unilamellar Liposomes; D009113:Muramidase
keywords: AFM; BAM; Dry-heated lysozyme; LPS monolayer; Langmuir film