title: Results of an International Interlaboratory Trial to Determine Twelve Allergens Using Real-time PCR- and ELISA-based Assays.
abstract: To elucidate the capability of laboratories to determine allergen contents, an international interlaboratory trial was conducted using meat products spiked with 12 allergens. The measurement uncertainty was calculated independent of the applied method simulating realistic situations when comparing analysis certificates from different laboratories. The measurement uncertainty was revealed to be in the best cases +/-100%, in the worst cases quantification exhibited a measurement uncertainty of higher than 200% making quantitative analysis impossible. The measurement uncertainty seemed to depend on the analyte and assays used.
authors.affiliation: Official Food Control Authority of the Canton of Zurich, Zürich, Switzerland. rene.koeppel@klzh.ch.
authors.forename: René
authors.initials: R
authors.lastname: Köppel
authors.affiliation: Swiss Quality Testing Services (SQTS) Courtepin, Switzerland.
authors.forename: Jürg
authors.initials: J
authors.lastname: Rentsch
authors.affiliation: Official Food Control Authority of the Canton Thurgau, Frauenfeld, Switzerland.
authors.forename: Jürg
authors.initials: J
authors.lastname: Ruf
authors.affiliation: Cantonal Office of Consumer Protection Aargau, Aarau, Switzerland.
authors.forename: Albert
authors.initials: A
authors.lastname: Eugster
authors.affiliation: Cantonal Office of Consumer Protection Bern, Switzerland.
authors.forename: Christoph
authors.initials: C
authors.lastname: Graf
authors.affiliation: Official Food Control Authority of the Canton of Zurich, Zürich, Switzerland.
authors.forename: Nora
authors.initials: N
authors.lastname: Felderer
authors.affiliation: State Institute of Chemical and Veterinarian Analysis Freiburg, Germany.
authors.forename: Klaus
authors.initials: K
authors.lastname: Pietsch
authors.affiliation: Cantonal Food Control Authority of Basel, Switzerland.
authors.forename: Evelyn
authors.initials: E
authors.lastname: Ilg
mesh_terms: D000485:Allergens / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay; D007753:Laboratories; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D000485:Allergens