title: Enzymatically controlled material design with casein--from defined films to localized deposition of particles.
abstract: A new concept for deposition and material design of coatings from biological compounds is presented. An enzymatic reaction triggers the specific coagulation of particles on a support surface. The first examined model system is casein and is based on the natural rennet reaction as applied in the process of cheese-making. The aspartic protease chymosin is immobilized on a support surface and cleaves the hydrophilic parts of the casein micelles, inducing deposition. The concept allows for a high level of control over film characteristics and enables the formation of site-specific film structures. The variability rages from formation of casein films with several micrometers film thickness to the targeted deposition of casein micelles.
authors.affiliation: University of Paderborn, Department of Chemistry - Coating Materials & Polymers, Paderborn, Germany. Electronic address: oliver.strube@upb.de.
authors.forename: Oliver I
authors.initials: OI
authors.lastname: Strube
authors.affiliation: University of Paderborn, Department of Chemistry - Coating Materials & Polymers, Paderborn, Germany.
authors.forename: Arne A
authors.initials: AA
authors.lastname: Rüdiger
authors.affiliation: University of Paderborn, Department of Chemistry - Coating Materials & Polymers, Paderborn, Germany.
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Bremser
mesh_terms: D001709:Biotechnology / Q000379:methods*; D002364:Caseins / Q000737:chemistry*; D012085:Chymosin / Q000737:chemistry; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D004800:Enzymes, Immobilized / Q000737:chemistry*; D004867:Equipment Design; D010316:Particle Size
chemical_list: D002364:Caseins; D020099:Coated Materials, Biocompatible; D004800:Enzymes, Immobilized; D012085:Chymosin
keywords: Casein; Coatings; Enzyme immobilization; Enzymes; Protein