title: Cryopreservation of rainbow trout Oncorhynchus mykiss spermatozoa: effects of extender supplemented with different antioxidants on sperm motility, velocity and fertility.
abstract: In present study, it was examined whether addition of different antioxidants to the cryopreservation extenders had an effect on semen post-thaw fertility and motility in rainbow trout (Oncorhynchus mykiss) and also it was investigated the sperm characteristics post-thaw sperm characteristics and fertility. The collected semen was pooled to minimize individual variation. Each pooled ejaculate was split into 12 equal aliquots and diluted with base extenders supplemented with the antioxidants, and a base extender with no additives (control). The pooled semen samples diluted at the ratio of 1:10 by the extenders were subjected to cryopreservation. Antioxidants were separately added to the extenders (one per experimental group): catalase (250 U/l), superoxide dismutase (250 U/l), peroxidase (250 U/l), oxidized glutathione (1.5 mmol/l), reduced glutathione (1.5 mmol/l), L-methionine (1.5 mmol/l), uric acid (0.25 mmol/l), L-ascorbic acid (0.5 mmol/l), α-tocopherol (2.0 mmol/l), β-carotene (0.5 mmol/l) and carnitine (0.5 mmol/l). After dilution the semen was aspirated into 0.25 ml straws, the straws were placed on the tray, frozen for 10 min, and plunged into liquid nitrogen. Our results indicated that the post-thaw motility rate increased in extenders supplemented with uric acid, L-methionine, SOD, L-carnitine, α-tocopherol and L-reduced glutathione (p<0.05). The motility duration of frozen thawed semen increased in extenders supplemented with uric acid, L-methionine, SOD, α-tocopherol and L-reduced glutathione (p<0.05). Fertilization rate and hatching rate of frozen-thawed semen was not affected by the tested antioxidants. Consequently, the tested antioxidants affected the motility parameters and cryopreservation extenders could be supplement with antioxidants. This study suggested usage of antioxidants in the cryopreservation of rainbow trout.
authors.affiliation: Tunceli University, Fisheries Faculty, 62000 Tunceli, Turkey. Electronic address: filizkutluyer@hotmail.com.
authors.forename: Filiz
authors.initials: F
authors.lastname: Kutluyer
authors.affiliation: Tunceli University, Fisheries Faculty, 62000 Tunceli, Turkey.
authors.forename: Murathan
authors.initials: M
authors.lastname: Kayim
authors.affiliation: Muğla Sıtkı Koçman University, Faculty of Fisheries, 48000 Muğla, Turkey.
authors.forename: Fatih
authors.initials: F
authors.lastname: Öğretmen
authors.affiliation: Aksaray University, Faculty of Veterinary Medicine, Department of Reproduction and Artificial Insemination, Aksaray, Turkey.
authors.forename: Serhat
authors.initials: S
authors.lastname: Büyükleblebici
authors.affiliation: Aksaray Vocational School, Aksaray University, Aksaray, Turkey.
authors.forename: P Barbaros
authors.initials: PB
authors.lastname: Tuncer
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D015925:Cryopreservation / Q000379:methods / Q000662:veterinary*; D005260:Female; D005298:Fertility / Q000187:drug effects; D008297:Male; D017686:Oncorhynchus mykiss / Q000502:physiology*; D013081:Sperm Motility / Q000187:drug effects; D013094:Spermatozoa / Q000166:cytology* / Q000187:drug effects
chemical_list: D000975:Antioxidants
keywords: Antioxidant; Oncorhynchus mykiss; Rainbow trout; Sperm quality