title: Kentomonas gen. n., a new genus of endosymbiont-containing trypanosomatids of Strigomonadinae subfam. n.
abstract: Compared to their relatives, the diversity of endosymbiont-containing Trypanosomatidae remains under-investigated, with only two new species described in the past 25 years, bringing the total to six. The possible reasons for such a poor representation of this group are either their overall scarcity or susceptibility of their symbionts to antibiotics that are traditionally used for cultivation of flagellates. In this work we describe the isolation, cultivation, as well as morphological and molecular characterization of a novel endosymbiont-harboring trypanosomatid species, Kentomonas sorsogonicus sp. n. The newly erected genus Kentomonas gen. n. shares many common features with the genera Angomonas and Strigomonas, such as the presence of an extensive system of peripheral mitochondrial branches distorting the corset of subpellicular microtubules, large and loosely packed kinetoplast, and a rudimentary paraflagellar rod. Here we also propose to unite all endosymbiont-bearing trypanosomatids into the new subfamily Strigomonadinae subfam. n.
authors.affiliation: Department of Parasitology, Faculty of Sciences, Charles University, Prague, Czech Republic; Biology Centre, Institute of Parasitology, Czech Academy of Sciences, České Budějovice (Budweis), Czech Republic.
authors.forename: Jan
authors.initials: J
authors.lastname: Votýpka
authors.affiliation: Life Science Research Centre, Faculty of Science, University of Ostrava, Ostrava, Czech Republic; Zoological Institute of the Russian Academy of Sciences, St. Petersburg, Russia.
authors.forename: Alexei Yu
authors.initials: AY
authors.lastname: Kostygov
authors.affiliation: Life Science Research Centre, Faculty of Science, University of Ostrava, Ostrava, Czech Republic.
authors.forename: Natalya
authors.initials: N
authors.lastname: Kraeva
authors.affiliation: Life Science Research Centre, Faculty of Science, University of Ostrava, Ostrava, Czech Republic.
authors.forename: Anastasiia
authors.initials: A
authors.lastname: Grybchuk-Ieremenko
authors.affiliation: Biology Centre, Institute of Parasitology, Czech Academy of Sciences, České Budějovice (Budweis), Czech Republic.
authors.forename: Martina
authors.initials: M
authors.lastname: Tesařová
authors.affiliation: Life Science Research Centre, Faculty of Science, University of Ostrava, Ostrava, Czech Republic.
authors.forename: Danyil
authors.initials: D
authors.lastname: Grybchuk
authors.affiliation: Biology Centre, Institute of Parasitology, Czech Academy of Sciences, České Budějovice (Budweis), Czech Republic; Faculty of Science, University of South Bohemia, České Budějovice (Budweis), Czech Republic.
authors.forename: Julius
authors.initials: J
authors.lastname: Lukeš
authors.affiliation: Biology Centre, Institute of Parasitology, Czech Academy of Sciences, České Budějovice (Budweis), Czech Republic; Life Science Research Centre, Faculty of Science, University of Ostrava, Ostrava, Czech Republic. Electronic address: vyacheslav.yurchenko@osu.cz.
authors.forename: Vyacheslav
authors.initials: V
authors.lastname: Yurchenko
mesh_terms: D000818:Animals; D001499:Bayes Theorem; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000302:isolation & purification; D004279:DNA, Viral / Q000302:isolation & purification; D016013:Likelihood Functions; D008957:Models, Genetic; D010802:Phylogeny; D016053:RNA, Protozoan / Q000235:genetics; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D058493:Sarcophagidae / Q000469:parasitology; D017422:Sequence Analysis, DNA; D013045:Species Specificity; D013559:Symbiosis / Q000235:genetics*; D014351:Trypanosomatina / Q000145:classification* / Q000302:isolation & purification / Q000382:microbiology / Q000821:virology
chemical_list: D004269:DNA, Bacterial; D004279:DNA, Viral; D016053:RNA, Protozoan; D012337:RNA, Ribosomal, 18S
keywords: Kentomonas; Trypanosomatidae; bacterial endosymbionts; phylogeny.