title: Effects of ectoine on behavioural, physiological and biochemical parameters of Daphnia magna.
abstract: Ectoine (ECT) is a compatible solute produced by soil, marine and freshwater bacteria in response to stressful factors. The purpose of our study was to determine the possible toxic influence of ECT on Daphnia magna. We determined the following endpoints: survival rate during exposure and recovery, swimming performance, heart rate, thoracic limb movement determined by image analysis, haemoglobin level by ELISA assay, catalase and nitric oxide species (NOx) by spectrophotometric methods. The results showed 80% survival of daphnids exposed to 50mg/L of ECT after 24h and 10% after 90h, however lower concentrations of ECT were well tolerated. A concentration-dependent reduction of swimming velocity was noted at 24 and 48h of the exposure. ECT (at 2.5 and 4mg/L) induced an increase of heart rate and thoracic limb movement (at 2.5, 4 and 20mg/L) after 24h. After 10h of the exposure to ECT daphnids showed a concentration-dependent increase of haemoglobin level synthesized and accumulated in the epipodite epithelia. After 24h we noted a concentration-dependent decrease of haemoglobin level and its lowest value was found after 48h of the exposure. ECT at a concentration of 20 and 25mg/L slightly stimulated catalase activity after 24h. NOx level was also increased after 10h of the exposure to 20 and 25mg/L of ECT reaching maximal activity after 24h. Our results suggest that ECT possesses some modulatory potential on the behaviour, physiology and biochemical parameters in daphnids.
authors.affiliation: Department of Physiology and Ecotoxicology, Faculty of Biotechnology and Environmental Sciences, The John Paul II Catholic University of Lublin, Kontstantynow 1 "I", 20-708 Lublin, Poland. Electronic address: adambownik@wp.pl.
authors.forename: Adam
authors.initials: A
authors.lastname: Bownik
authors.affiliation: Department of Biochemistry Environmental Chemistry, Faculty of Biotechnology and Environmental Sciences, The John Paul II Catholic University of Lublin, Kontstantynow 1 "I", 20-708 Lublin, Poland.
authors.forename: Zofia
authors.initials: Z
authors.lastname: Stępniewska
authors.affiliation: Department of Physiology and Ecotoxicology, Faculty of Biotechnology and Environmental Sciences, The John Paul II Catholic University of Lublin, Kontstantynow 1 "I", 20-708 Lublin, Poland.
authors.forename: Tadeusz
authors.initials: T
authors.lastname: Skowroński
mesh_terms: D000599:Amino Acids, Diamino / Q000009:adverse effects*; D000818:Animals; D002374:Catalase / Q000378:metabolism; D003621:Daphnia / Q000187:drug effects* / Q000502:physiology*; D006339:Heart Rate / Q000187:drug effects; D009569:Nitric Oxide / Q000378:metabolism; D015996:Survival Rate; D013550:Swimming / Q000502:physiology
chemical_list: D000599:Amino Acids, Diamino; D009569:Nitric Oxide; C045628:ectoine; D002374:Catalase
keywords: Compatible solute; Daphnia magna; Ectoine