title: Type I interferon-mediated immune response against influenza A virus is attenuated in the absence of p53.
abstract: Influenza A virus (IAV) infection induces secretion of type I interferon (IFN) and activation of p53, which play essential roles in the host defense against tumor development and viral infection. In this study, we knocked down p53 expression by RNA interference. The expression levels of IFN-stimulated genes (ISGs) including IFN regulatory factor (IRF) 5, IRF9, ISG15, ISG20, guanylate-binding protein 1, retinoic acid-inducible gene-I and 2'-5'-oligoadenylate synthetase 1 were significantly attenuated in response to IAV infection and IFN-α stimulation in p53-knockdown cells. This attenuated expression of ISGs was associated with enhanced replication of IAV. Pretreatment of p53-knockdown cells with IFN-α failed to inhibit IAV replication, indicating impaired antiviral activity. These findings indicate that p53 plays an essential role in the enhancement of the type I IFN-mediated immune response against IAV infection.
authors.affiliation: Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, No. 518, Ziyue Road, Shanghai 200241, PR China; Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, No. 1, Xujiaping Road, Lanzhou 730046, PR China.
authors.forename: Zixiang
authors.initials: Z
authors.lastname: Zhu
authors.affiliation: Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, No. 518, Ziyue Road, Shanghai 200241, PR China.
authors.forename: Yifan
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, No. 518, Ziyue Road, Shanghai 200241, PR China.
authors.forename: Jianchao
authors.initials: J
authors.lastname: Wei
authors.affiliation: Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, No. 518, Ziyue Road, Shanghai 200241, PR China.
authors.forename: Donghua
authors.initials: D
authors.lastname: Shao
authors.affiliation: Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, No. 518, Ziyue Road, Shanghai 200241, PR China.
authors.forename: Zixue
authors.initials: Z
authors.lastname: Shi
authors.affiliation: Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, No. 518, Ziyue Road, Shanghai 200241, PR China.
authors.forename: Beibei
authors.initials: B
authors.lastname: Li
authors.affiliation: Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, No. 518, Ziyue Road, Shanghai 200241, PR China.
authors.forename: Ke
authors.initials: K
authors.lastname: Liu
authors.affiliation: Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, No. 518, Ziyue Road, Shanghai 200241, PR China.
authors.forename: Yafeng
authors.initials: Y
authors.lastname: Qiu
authors.affiliation: Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, No. 1, Xujiaping Road, Lanzhou 730046, PR China.
authors.forename: Haixue
authors.initials: H
authors.lastname: Zheng
authors.affiliation: Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, No. 518, Ziyue Road, Shanghai 200241, PR China. Electronic address: zhiyongma@shvri.ac.cn.
authors.forename: Zhiyong
authors.initials: Z
authors.lastname: Ma
mesh_terms: D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology; D002460:Cell Line; D002642:Chick Embryo; D004285:Dogs; D015870:Gene Expression; D055785:Gene Knockdown Techniques; D016158:Genes, p53; D054884:Host-Pathogen Interactions / Q000235:genetics / Q000276:immunology; D006801:Humans; D009980:Influenza A virus / Q000276:immunology* / Q000472:pathogenicity* / Q000502:physiology; D007370:Interferon Type I / Q000235:genetics / Q000276:immunology*; D000077190:Interferon alpha-2; D016898:Interferon-alpha / Q000494:pharmacology; D034741:RNA, Small Interfering / Q000235:genetics; D011994:Recombinant Proteins / Q000494:pharmacology; D016159:Tumor Suppressor Protein p53 / Q000172:deficiency* / Q000235:genetics / Q000276:immunology; D014779:Virus Replication
chemical_list: D000998:Antiviral Agents; D007370:Interferon Type I; D000077190:Interferon alpha-2; D016898:Interferon-alpha; D034741:RNA, Small Interfering; D011994:Recombinant Proteins; D016159:Tumor Suppressor Protein p53
keywords: Antiviral immune response; Influenza A virus; Interferon-stimulated genes; Type I interferon; p53