title: Kinetics of the early events of GPCR signalling.
abstract: Neurotensin receptor type 1 (NTS1) is a G protein-coupled receptor (GPCR) that affects cellular responses by initiating a cascade of interactions through G proteins. The kinetic details for these interactions are not well-known. Here, NTS1-nanodisc-Gαs and Gαi1 interactions were studied. The binding affinities of Gαi1 and Gαs to NTS1 were directly measured by surface plasmon resonance (SPR) and determined to be 15±6 nM and 31±18 nM, respectively. This SPR configuration permits the kinetics of early events in signalling pathways to be explored and can be used to initiate descriptions of the GPCR interactome.
authors.affiliation: Biomembrane Structure Unit, Biochemistry Department, University of Oxford, South Parks Road, Oxford OX1 3QU, UK.
authors.forename: Roslin J
authors.initials: RJ
authors.lastname: Adamson
authors.affiliation: Biomembrane Structure Unit, Biochemistry Department, University of Oxford, South Parks Road, Oxford OX1 3QU, UK. Electronic address: anthony.watts@bioch.ox.ac.uk.
authors.forename: Anthony
authors.initials: A
authors.lastname: Watts
mesh_terms: D002462:Cell Membrane / Q000378:metabolism; D019206:GTP-Binding Protein alpha Subunits, Gi-Go / Q000737:chemistry / Q000378:metabolism; D019205:GTP-Binding Protein alpha Subunits, Gs / Q000737:chemistry / Q000378:metabolism; D007700:Kinetics; D008958:Models, Molecular; D049329:Nanostructures; D011487:Protein Conformation; D018028:Receptors, Neurotensin / Q000737:chemistry / Q000378:metabolism*; D015398:Signal Transduction
chemical_list: D018028:Receptors, Neurotensin; D019206:GTP-Binding Protein alpha Subunits, Gi-Go; D019205:GTP-Binding Protein alpha Subunits, Gs
keywords: Electron microscopy; G protein; G protein-coupled receptor; Nanodisc; Surface plasmon resonance