title: Transcriptomic response to estrogen exposure in the male Zhikong scallop, Chlamys farreri.
abstract: The transcriptomes of Zhikong scallop exposed to 17β-estradiol were determined using the Roche/454. A total of 51,997 unigenes, representing 45,030 contigs and 6967 singlets were obtained. And 14,028, 19,798 and 14,981 of these unigenes were annotated from the non-redundant nucleic acid database, non-redundant protein database and Swiss protein database, respectively. A total of 10,699 unigenes were further annotated to biological processes (9080), molecular functions (8692) and cellular components (7829) using the GO, and 8945 unigenes were mapped to biological pathways including the metabolism (2862) and genetic information processing (2263). Most importantly, 16,692 unigenes and 18,686 unigenes in testis, and 10,492 unigenes and 13,186 unigenes in digestive gland were up-regulated significantly after exposure to 50 and 500 ng E2/L; while 10,212 unigenes and 9409 unigenes in testis and 10,629 unigenes and 9463 unigenes in digestive gland were down-regulated. These valuable information provides insights into the mechanisms in invertebrate exposure to EDCs.
authors.affiliation: State Environmental Protection Key Laboratory of Estuarine and Coastal Research, Chinese Research Academy of Environmental Sciences, Beijing 100012, China.
authors.forename: Li-Hui
authors.initials: LH
authors.lastname: An
authors.affiliation: State Environmental Protection Key Laboratory of Estuarine and Coastal Research, Chinese Research Academy of Environmental Sciences, Beijing 100012, China. Electronic address: zhengbh_craes@163.com.
authors.forename: Bing-Hui
authors.initials: BH
authors.lastname: Zheng
authors.affiliation: State Environmental Protection Key Laboratory of Estuarine and Coastal Research, Chinese Research Academy of Environmental Sciences, Beijing 100012, China.
authors.forename: Rui-Zhi
authors.initials: RZ
authors.lastname: Liu
authors.affiliation: State Environmental Protection Key Laboratory of Estuarine and Coastal Research, Chinese Research Academy of Environmental Sciences, Beijing 100012, China; Tianjin Agricultural College, Tianjin 300384, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Fan
authors.affiliation: Tianjin Agricultural College, Tianjin 300384, China.
authors.forename: Qing-Kui
authors.initials: QK
authors.lastname: Wang
authors.affiliation: Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing 100029, China.
authors.forename: Ying-Feng
authors.initials: YF
authors.lastname: Luo
mesh_terms: D000818:Animals; D004958:Estradiol / Q000494:pharmacology*; D004967:Estrogens / Q000494:pharmacology*; D020869:Gene Expression Profiling; D008297:Male; D049897:Pectinidae / Q000187:drug effects* / Q000235:genetics; D059467:Transcriptome / Q000187:drug effects*
chemical_list: D004967:Estrogens; D004958:Estradiol
keywords: 454 Sequencing; Estrogen; Mechanism; Transcriptome; Zhikong scallop Chlamys farreri