title: Molecularly imprinted polymers with synthetic dummy template for simultaneously selective removal and enrichment of ginkgolic acids from Ginkgo biloba L. leaves extracts.
abstract: Dummy molecularly imprinted polymers (DMIPs) for simultaneously selective removal and enrichment of ginkgolic acids (GAs) during the processing of Ginkgo biloba leaves have been prepared. Two dummy template molecule with similar structural skeleton to GAs, 6-methoxysalicylic acid (MOSA, DT-1) and 6-hexadecyloxysalicylic acid (HOSA, DT-2), have been designed and synthesized. The performance of the DMIPs and NIPs were evaluated including selective recognition capacity, adsorption isotherm, and adsorption kinetics. The selective recognition capacity of the three GAs with four analogues on the sorbents illustrated that the DMIPs sorbents have high specificity for GAs. An efficient method based on DMIP-HOSA coupled with solid-phase extraction (SPE) was developed for simultaneously selective removal and enrichment of ginkgolic acids (GAs) during the processing of Ginkgo biloba leaves. The method showed excellent recoveries (82.5-88.7%) and precision (RSD 0.5-2.6%, n=5) for licorice extracts, Gastrodia elata extracts and pepper extracts spiked at three concentration levels each (50, 100, 200 μg mL(-1)). The results indicated that GAs and standardized Ginkgo biloba leaves extracts could be obtained simultaneously through the DMIP-SPE.
authors.affiliation: Shandong Analysis and Test Center, Shandong Academy of Sciences, 19 Keyuan Street, Jinan, 250014, China.
authors.forename: Wenhua
authors.initials: W
authors.lastname: Ji
authors.affiliation: College of Food Science and Engineering, Shandong Agricultural University, Taian, 270018, China.
authors.forename: Xiuli
authors.initials: X
authors.lastname: Ma
authors.affiliation: College of Food Science and Engineering, Shandong Agricultural University, Taian, 270018, China.
authors.forename: Hongkai
authors.initials: H
authors.lastname: Xie
authors.affiliation: College of Food Science and Engineering, Shandong Agricultural University, Taian, 270018, China.
authors.forename: Lingxiao
authors.initials: L
authors.lastname: Chen
authors.affiliation: Shandong Analysis and Test Center, Shandong Academy of Sciences, 19 Keyuan Street, Jinan, 250014, China; College of Food Science and Engineering, Shandong Agricultural University, Taian, 270018, China. Electronic address: wangx@sdas.org.
authors.forename: Xiao
authors.initials: X
authors.lastname: Wang
authors.affiliation: Shandong Analysis and Test Center, Shandong Academy of Sciences, 19 Keyuan Street, Jinan, 250014, China.
authors.forename: Hengqiang
authors.initials: H
authors.lastname: Zhao
authors.affiliation: National Resource Center for Chinese Materia Medica, State Key Laboratory Breeding Base of Dao-di Herbs, China Academy of Chinese Medical Sciences, Beijing, 100700, China. Electronic address: luqihuang@126.com.
authors.forename: Luqi
authors.initials: L
authors.lastname: Huang
mesh_terms: D000327:Adsorption; D020441:Ginkgo biloba / Q000737:chemistry*; D007700:Kinetics; D054802:Molecular Imprinting / Q000379:methods*; D010936:Plant Extracts / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry; D011108:Polymers / Q000737:chemistry*; D012459:Salicylates / Q000302:isolation & purification*; D052616:Solid Phase Extraction / Q000379:methods
chemical_list: D010936:Plant Extracts; D011108:Polymers; D012459:Salicylates; C112485:ginkgolic acid
keywords: Dummy template; Ginkgo biloba L; Ginkgolic acids; Molecular imprinted polymers; Solid-phase extraction