title: Upregulation of microRNA-106b is associated with poor prognosis in hepatocellular carcinoma.
abstract: BACKGROUND
  MicroRNA-106b (miR-106b) is a member of the miR-106b ~ 25 cluster. It has been reported that miR-106b acts as an oncogene and is upregulated in many human cancers. However, the prognostic value of miR-106b in hepatocellular carcinoma (HCC) remains unclear. The aim of this study was to investigate the clinical significance of miR-106b expression in HCC.
  
  
  METHODS
  We determined the expression level of miR-106b in 104 cases of paired HCC and adjacent non-tumor tissues by quantitative real-time PCR (qRT-PCR). The correlation between miR-106b expression and prognosis of HCC was studied by univariate and multivariate analysis. Multivariate analysis of the prognostic factors was performed with Cox proportional hazards model.
  
  
  RESULTS
  MiR-106b expression was significantly upregulated in as high as 76.0% of HCC tissues, compared with their non-tumor counterparts (P < 0.001). High miR-106b expression was significantly associated with large tumor size (P = 0.019) and vascular invasion (P = 0.016). Kaplan-Meier analysis showed that patients with high miR-106b expression had a worse overall survival than patients with low miR-106b expression (log-rank P = 0.004). The multivariate Cox regression analysis indicated that miR-106b expression was an independent prognostic factor for overall survival (HR, 2.002; 95% CI, 1.130-6.977; P = 0.027).
  
  
  CONCLUSIONS
  Our data indicated that miR-106b expression was significantly upregulated in HCC and could serve as a potential unfavorable prognostic biomarker.
  The virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/13000_2014_226.
authors.affiliation: State Key Laboratory of Oncology in South China, Guangzhou, China. libinkui@mail.sysu.edu.cn.|Department of Hepatobiliary Oncology, Sun Yat-sen University Cancer Center, 651 Dongfeng Road East, Guangzhou, Guangdong, 510060, China. libinkui@mail.sysu.edu.cn.
authors.forename: Bin-Kui
authors.initials: BK
authors.lastname: Li
authors.affiliation: State Key Laboratory of Oncology in South China, Guangzhou, China. pinzhuhuang@163.com.|Department of Hepatobiliary Oncology, Sun Yat-sen University Cancer Center, 651 Dongfeng Road East, Guangzhou, Guangdong, 510060, China. pinzhuhuang@163.com.
authors.forename: Pin-Zhu
authors.initials: PZ
authors.lastname: Huang
authors.affiliation: State Key Laboratory of Oncology in South China, Guangzhou, China. jiliangqiu@163.com.|Department of Hepatobiliary Oncology, Sun Yat-sen University Cancer Center, 651 Dongfeng Road East, Guangzhou, Guangdong, 510060, China. jiliangqiu@163.com.
authors.forename: Ji-Liang
authors.initials: JL
authors.lastname: Qiu
authors.affiliation: State Key Laboratory of Oncology in South China, Guangzhou, China. liaoyd@sysucc.org.cn.|Department of Hepatobiliary Oncology, Sun Yat-sen University Cancer Center, 651 Dongfeng Road East, Guangzhou, Guangdong, 510060, China. liaoyd@sysucc.org.cn.
authors.forename: Ya-Di
authors.initials: YD
authors.lastname: Liao
authors.affiliation: State Key Laboratory of Oncology in South China, Guangzhou, China. 05213625hj@sina.com.|Department of Hepatobiliary Oncology, Sun Yat-sen University Cancer Center, 651 Dongfeng Road East, Guangzhou, Guangdong, 510060, China. 05213625hj@sina.com.
authors.forename: Jian
authors.initials: J
authors.lastname: Hong
authors.affiliation: State Key Laboratory of Oncology in South China, Guangzhou, China. yuanyf@mail.sysu.edu.cn.|Department of Hepatobiliary Oncology, Sun Yat-sen University Cancer Center, 651 Dongfeng Road East, Guangzhou, Guangdong, 510060, China. yuanyf@mail.sysu.edu.cn.
authors.forename: Yun-Fei
authors.initials: YF
authors.lastname: Yuan
mesh_terms: D014408:Biomarkers, Tumor / Q000235:genetics*; D006528:Carcinoma, Hepatocellular / Q000235:genetics* / Q000473:pathology; D016009:Chi-Square Distribution; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D053208:Kaplan-Meier Estimate; D008113:Liver Neoplasms / Q000235:genetics* / Q000473:pathology; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D015999:Multivariate Analysis; D009361:Neoplasm Invasiveness; D011237:Predictive Value of Tests; D011379:Prognosis; D016016:Proportional Hazards Models; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012307:Risk Factors; D047368:Tumor Burden; D015854:Up-Regulation
chemical_list: D014408:Biomarkers, Tumor; C542541:MIRN106 microRNA, human; D035683:MicroRNAs