title: An on-line HPLC-FRSD system for rapid evaluation of the total antioxidant capacity of Citrus fruits.
abstract: An on-line high performance liquid chromatography-free radical scavenging detection (HPLC-FRSD) system was developed for rapidly evaluating the total antioxidant capacity (TAC) of Citrus fruits. With the system, all samples can be analyzed within 5 min. The on-line HPLC-FRSD system has low limits of detection (0.001-0.010 mg mL(-1)) and quantification (0.005-0.020 mg mL(-1)), excellent recovery rate (90.44-115.72%), stability (RSD < 15.80%), reproducibility (RSD < 2%), and precision (RSD < 2%). Using a guard column instead of an analytic column, this new on-line HPLC-FRSD system performed better than the existing on-line HPLC methods in the analysis of the TAC of Citrus. Compared with the conventional off-line 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis (3-ethylbenzthiozoline-6)-sulphonic acid (ABTS) radical scavenging methods, our newly developed system is faster and more robust. The methodology can be a good alternative for analysis TACs of Citrus fruits and potentially for other plants and plant-based products.
authors.affiliation: College of Horticulture and Landscape Architecture, Southwest University, Chongqing 400716, China; Key Laboratory of Horticulture for Southern Mountainous Regions, Ministry of Education, Chongqing 400715, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Zhang
authors.affiliation: College of Horticulture and Landscape Architecture, Southwest University, Chongqing 400716, China; Key Laboratory of Horticulture for Southern Mountainous Regions, Ministry of Education, Chongqing 400715, China.
authors.forename: Wanpeng
authors.initials: W
authors.lastname: Xi
authors.affiliation: College of Horticulture and Landscape Architecture, Southwest University, Chongqing 400716, China.
authors.forename: Yifei
authors.initials: Y
authors.lastname: Yang
authors.affiliation: College of Horticulture and Landscape Architecture, Southwest University, Chongqing 400716, China.
authors.forename: Xingyu
authors.initials: X
authors.lastname: Zhou
authors.affiliation: College of Horticulture and Landscape Architecture, Southwest University, Chongqing 400716, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Liu
authors.affiliation: College of Horticulture and Landscape Architecture, Southwest University, Chongqing 400716, China.
authors.forename: Shanshan
authors.initials: S
authors.lastname: Yin
authors.affiliation: College of Horticulture and Landscape Architecture, Southwest University, Chongqing 400716, China.
authors.forename: Jinmei
authors.initials: J
authors.lastname: Zhang
authors.affiliation: College of Horticulture and Landscape Architecture, Southwest University, Chongqing 400716, China; Key Laboratory of Horticulture for Southern Mountainous Regions, Ministry of Education, Chongqing 400715, China. Electronic address: zqzhou2013@swu.edu.cn.
authors.forename: Zhiqin
authors.initials: Z
authors.lastname: Zhou
mesh_terms: D000975:Antioxidants / Q000032:analysis*; D052160:Benzothiazoles / Q000032:analysis*; D001713:Biphenyl Compounds; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002957:Citrus / Q000737:chemistry*; D010851:Picrates; D010936:Plant Extracts / Q000032:analysis*; D015203:Reproducibility of Results; D013451:Sulfonic Acids / Q000032:analysis*
chemical_list: D000975:Antioxidants; D052160:Benzothiazoles; D001713:Biphenyl Compounds; D010851:Picrates; D010936:Plant Extracts; D013451:Sulfonic Acids; C002502:2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid; C004931:1,1-diphenyl-2-picrylhydrazyl
keywords: Citrus fruits; Evaluation; On-line HPLC-FRSD; Total antioxidant capacity