title: A novel controlled release system-based homogeneous immunoassay protocol for SCCA using magnetic mesoporous Fe3O4 as a nanocontainer and aminated polystyrene microspheres as a molecular gate.
abstract: A novel controlled release system-based homogeneous immunoassay protocol (CRSHIP) was developed for the detection of squamous cell carcinoma antigen (SCCA). The positively charged aminated polystyrene microsphere (APSM) used as molecular gate was capped on the pores of the negatively charged antibody-conjugated magnetic mesoporous Fe3O4 (Ab-Fe3O4) by electrostatic interaction to achieve encapsulation of toluidine blue (TB). With the addition of SCCA, APSM was replaced from Ab-Fe3O4 due to the specific binding of antibody to antigen, and then the trapped TB was released from the pores. The released TB could be detected by square wave voltammetry and the signal could be correlated with the amount of SCCA. The developed CRSHIP displayed a high current response, a relatively wide linear range (from 0.001 to 4.0ng/mL) and a low detection limit (0.33pg/mL) for the detection of SCCA. The method showed high selectivity and good reproducibility. In addition, the CRSHIP provided a simple, fast and sensitive approach for the detection of SCCA in serum sample.
authors.affiliation: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Gao
authors.affiliation: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, China.
authors.forename: Zhankui
authors.initials: Z
authors.lastname: Guo
authors.affiliation: School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, China.
authors.forename: Siqi
authors.initials: S
authors.lastname: Yu
authors.affiliation: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, China.
authors.forename: Fengjie
authors.initials: F
authors.lastname: Su
authors.affiliation: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, China.
authors.forename: Hongmin
authors.initials: H
authors.lastname: Ma
authors.affiliation: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Du
authors.affiliation: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Wei
authors.affiliation: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, China.
authors.forename: Xuehui
authors.initials: X
authors.lastname: Pang
mesh_terms: D000906:Antibodies / Q000737:chemistry / Q000276:immunology; D000951:Antigens, Neoplasm / Q000097:blood / Q000276:immunology / Q000302:isolation & purification*; D015374:Biosensing Techniques; D005290:Ferric Compounds / Q000737:chemistry; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D008863:Microspheres; D011137:Polystyrenes / Q000737:chemistry; D015843:Serpins / Q000097:blood / Q000276:immunology / Q000302:isolation & purification*
chemical_list: D000906:Antibodies; D000951:Antigens, Neoplasm; D005290:Ferric Compounds; D058185:Magnetite Nanoparticles; D011137:Polystyrenes; D015843:Serpins; C051072:squamous cell carcinoma-related antigen; C000499:ferric oxide
keywords: Controlled release; Homogeneous immunoassay; Mesoporous Fe(3)O(4); Squamous cell carcinoma antigen