title: Spotted fever group Rickettsia in Amblyomma dubitatum tick from the urban area of Campo Grande, Mato Grosso do Sul, Brazil.
abstract: Rickettsia infection of each tick was evaluated by the hemolymph test and polymerase chain reaction (PCR) targeting gltA and ompA genes. All hemolymph tests were negative and PCR of one A. dubitatum detected both Rickettsia genes. Sequence of ompA exhibited a 99% identity with Rickettsia parkeri and R. africae and a 98% identity with R. sibirica. Rickettsia of the spotted fever group in A. dubitatum is described for the first time in an urban area within the municipality of Campo Grande in the state of Mato Grosso do Sul (MS), Brazil. This finding reinforces the importance of more detailed studies to determine the role of A. dubitatum in the transmission of spotted fever agents.
authors.affiliation: Programa de Pós-graduação em Doenças Infecciosas e Parasitárias, Faculdade de Medicina, Universidade Federal de Mato Grosso do Sul, Campo Grande, MS, Brazil.
authors.forename: Jaqueline
authors.initials: J
authors.lastname: Matias
authors.affiliation: Embrapa Beef Cattle, Av. Radio Maia 830, Vila Popular, Campo Grande, MS, Brazil.
authors.forename: Marcos Valério
authors.initials: MV
authors.lastname: Garcia
authors.affiliation: Programa de Pós-Graduação em Biotecnologia, Universidade Federal de Pelotas, Bolsista PNPD/CAPES, Brazil.
authors.forename: Rodrigo Casquero
authors.initials: RC
authors.lastname: Cunha
authors.affiliation: Programa de Pós-graduação em Doenças Infecciosas e Parasitárias, Faculdade de Medicina, Universidade Federal de Mato Grosso do Sul, Campo Grande, MS, Brazil.
authors.forename: André de Abreu Rangel
authors.initials: Ade A
authors.lastname: Aguirre
authors.affiliation: Embrapa Beef Cattle, Av. Radio Maia 830, Vila Popular, Campo Grande, MS, Brazil.
authors.forename: Jacqueline Cavalvante
authors.initials: JC
authors.lastname: Barros
authors.affiliation: Programa de Pós-graduação em Doenças Infecciosas e Parasitárias, Faculdade de Medicina, Universidade Federal de Mato Grosso do Sul, Campo Grande, MS, Brazil.
authors.forename: Bárbara Guimarães
authors.initials: BG
authors.lastname: Csordas
authors.affiliation: Embrapa Beef Cattle, Av. Radio Maia 830, Vila Popular, Campo Grande, MS, Brazil. Electronic address: renato.andreotti@embrapa.br.
authors.forename: Renato
authors.initials: R
authors.lastname: Andreotti
mesh_terms: D000818:Animals; D001096:Arachnid Vectors / Q000145:classification / Q000382:microbiology*; D001483:Base Sequence; D001938:Brazil / Q000453:epidemiology; D005260:Female; D005843:Geography; D006801:Humans; D026863:Ixodidae / Q000145:classification / Q000382:microbiology*; D008297:Male; D008969:Molecular Sequence Data; D010802:Phylogeny; D012281:Rickettsia / Q000235:genetics / Q000302:isolation & purification*; D012282:Rickettsia Infections / Q000382:microbiology* / Q000635:transmission; D017422:Sequence Analysis, DNA; D017282:Tick-Borne Diseases / Q000382:microbiology* / Q000635:transmission; D014504:Urban Health; D015047:Zoonoses
keywords: Amblyomma dubitatum; Campo Grande; Spotted fever group; Tick; Urban área; Zoonosis