title: BSA-stabilized Pt nanozyme for peroxidase mimetics and its application on colorimetric detection of mercury(II) ions.
abstract: Bovine serum albumin (BSA) is chosen as the nucleation templates to synthesize Pt-based peroxidase nanomimetics with the average diameter of 2.0nm. The efficient Pt nanozymes consist of 57% Pt(0) and 43% Pt(2+), which possess highly peroxidase-like activity with the Km values of 0.119mM and 41.8mM toward 3,3',5,5'-tetramethylbenzidine (TMB) and hydrogen peroxide (H2O2), respectively. Interestingly, Hg(2+) is able to down-regulate the enzymatic activity of Pt nanoparticles, mainly through the interactions between Hg(2+) and Pt(0). It is the first report to explore a colorimetric Hg(2+) sensing system on the basis of peroxidase mimicking activities of Pt nanoparticles. One of our most intriguing results is that BSA-stabilized Pt nanozymes demonstrate the ability to sense Hg(2+) ions in aqueous solution without significant interference from other metal ions. The Hg(2+) detection limit of 7.2nM is achieved with a linear response range of 0-120nM, and the developed sensing system is potentially applicable for quantitative determination of Hg(2+) in drinking water.
authors.affiliation: Key Laboratory for Green Chemical Technology MOE, Key Laboratory of Systems Bioengineering MOE, Collaborative Innovation Center of Chemical Science and Chemical Engineering (Tianjin), School of Chemical Engineering & Technology, Tianjin University, Tianjin 300072, PR China.
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.affiliation: Key Laboratory for Green Chemical Technology MOE, Key Laboratory of Systems Bioengineering MOE, Collaborative Innovation Center of Chemical Science and Chemical Engineering (Tianjin), School of Chemical Engineering & Technology, Tianjin University, Tianjin 300072, PR China.
authors.forename: Bin
authors.initials: B
authors.lastname: Chen
authors.affiliation: Key Laboratory for Green Chemical Technology MOE, Key Laboratory of Systems Bioengineering MOE, Collaborative Innovation Center of Chemical Science and Chemical Engineering (Tianjin), School of Chemical Engineering & Technology, Tianjin University, Tianjin 300072, PR China.
authors.forename: Haixiang
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Department of Chemistry and Biology, School of Pharmacy, Tianjin Medical University, Tianjin 300070, PR China.
authors.forename: Yanhua
authors.initials: Y
authors.lastname: Sun
authors.affiliation: Department of Chemistry and Biology, School of Pharmacy, Tianjin Medical University, Tianjin 300070, PR China.
authors.forename: Jun
authors.initials: J
authors.lastname: Wang
authors.affiliation: Key Laboratory for Green Chemical Technology MOE, Key Laboratory of Systems Bioengineering MOE, Collaborative Innovation Center of Chemical Science and Chemical Engineering (Tianjin), School of Chemical Engineering & Technology, Tianjin University, Tianjin 300072, PR China.
authors.forename: Jinli
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Key Laboratory for Green Chemical Technology MOE, Key Laboratory of Systems Bioengineering MOE, Collaborative Innovation Center of Chemical Science and Chemical Engineering (Tianjin), School of Chemical Engineering & Technology, Tianjin University, Tianjin 300072, PR China. Electronic address: fuyan@tju.edu.cn.
authors.forename: Yan
authors.initials: Y
authors.lastname: Fu
mesh_terms: D040761:Biomimetic Materials / Q000737:chemistry; D015374:Biosensing Techniques / Q000379:methods*; D003124:Colorimetry; D060766:Drinking Water / Q000032:analysis*; D006046:Gold / Q000737:chemistry; D006861:Hydrogen Peroxide / Q000737:chemistry; D007477:Ions / Q000302:isolation & purification*; D008628:Mercury / Q000302:isolation & purification*; D053768:Metal Nanoparticles / Q000737:chemistry; D009195:Peroxidase / Q000737:chemistry; D010984:Platinum / Q000737:chemistry; D012710:Serum Albumin, Bovine / Q000737:chemistry
chemical_list: D060766:Drinking Water; D007477:Ions; D012710:Serum Albumin, Bovine; D010984:Platinum; D006046:Gold; D006861:Hydrogen Peroxide; D009195:Peroxidase; D008628:Mercury
keywords: Hg(2+) detection; Nanoparticle; Nanozyme; Platinum; Protein