title: Construction and characterization of three wheat bacterial artificial chromosome libraries.
abstract: We have constructed three bacterial artificial chromosome (BAC) libraries of wheat cultivar Triticum aestivum Wangshuibai, germplasms T. monococcum TA2026 and TA2033. A total of 1,233,792,170,880 and 263,040 clones were picked and arrayed in 384-well plates. On the basis of genome sizes of 16.8 Gb for hexaploid wheat and 5.6 Gb for diploid wheat, the three libraries represented 9.05-, 2.60-, and 3.71-fold coverage of the haploid genomes, respectively. An improved descending pooling system for BAC libraries screening was established. This improved strategy can save 80% of the time and 68% of polymerase chain reaction (PCR) with the same successful rate as the universal 6D pooling strategy.
authors.affiliation: College of Agricultural Sciences, Nanjing Agricultural University, Nanjing 210095, China. caowenjin83@gmail.com.
authors.forename: Wenjin
authors.initials: W
authors.lastname: Cao
authors.affiliation: College of Agricultural Sciences, Nanjing Agricultural University, Nanjing 210095, China. bishengfu@126.com.
authors.forename: Bisheng
authors.initials: B
authors.lastname: Fu
authors.affiliation: College of Agricultural Sciences, Nanjing Agricultural University, Nanjing 210095, China. kunwu82@126.com.
authors.forename: Kun
authors.initials: K
authors.lastname: Wu
authors.affiliation: College of Agricultural Sciences, Nanjing Agricultural University, Nanjing 210095, China. nali310312@126.com.
authors.forename: Na
authors.initials: N
authors.lastname: Li
authors.affiliation: College of Agricultural Sciences, Nanjing Agricultural University, Nanjing 210095, China. yanzhou89@126.com.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: College of Agricultural Sciences, Nanjing Agricultural University, Nanjing 210095, China. zhongxiagao88@126.com.
authors.forename: Zhongxia
authors.initials: Z
authors.lastname: Gao
authors.affiliation: College of Agricultural Sciences, Nanjing Agricultural University, Nanjing 210095, China. musenlin88@126.com.
authors.forename: Musen
authors.initials: M
authors.lastname: Lin
authors.affiliation: College of Agricultural Sciences, Nanjing Agricultural University, Nanjing 210095, China. Guoqiangli82@126.com.
authors.forename: Guoqiang
authors.initials: G
authors.lastname: Li
authors.affiliation: College of Agricultural Sciences, Nanjing Agricultural University, Nanjing 210095, China. xinyiwu88@126.com.
authors.forename: Xinyi
authors.initials: X
authors.lastname: Wu
authors.affiliation: College of Agricultural Sciences, Nanjing Agricultural University, Nanjing 210095, China. zqm2@njau.edu.cn.
authors.forename: Zhengqiang
authors.initials: Z
authors.lastname: Ma
authors.affiliation: College of Agricultural Sciences, Nanjing Agricultural University, Nanjing 210095, China. hyjia@njau.edu.cn.
authors.forename: Haiyan
authors.initials: H
authors.lastname: Jia
mesh_terms: D022202:Chromosomes, Artificial, Bacterial / Q000235:genetics*; D018744:DNA, Plant / Q000235:genetics; D015723:Gene Library; D018745:Genome, Plant; D016133:Polymerase Chain Reaction; D014908:Triticum / Q000235:genetics*
chemical_list: D018744:DNA, Plant