title: Adhesion and proliferation of HeLa and fibroblast cells on chemically-modified gold surfaces.
abstract: The development of materials that allow proper functioning of cells on solid supports is directly relevant to the construction of living-cell biosensors. Both physical and chemical properties of the surfaces have been shown to be critical in this field. Our aim is to report correlations between chemical properties of surfaces and cell behavior by studying adhesion, viability and proliferation of fibroblasts and HeLa cells. Neither fibroblasts nor HeLa cells adhered to a hydrophobic surface. Fibroblasts were able to attach and proliferate well on all other surfaces tested. In contrast, on some surfaces where HeLa cells adhered and were viable, proliferation decreased by half while on others proliferation was not affected. Proliferation was significantly correlated with the level of adsorption of serum proteins on the surface (quantified by surface plasmon resonance), but not with surface wettability (water contact angle). Interestingly, surfaces modified with COOH and HSO3 groups were the ones that favored most protein adsorption and allowed the best measures for HeLa cell proliferation. The decrease of HeLa cell proliferation on surfaces covered with poly-L-lysine (PL) was related with the profile of integrin expression. Compared to a polystyrene control surface, there was an increase in αV and αVβ3 and a decrease in α2 and α3, indicating that migration rather than proliferation could be favored on PL functionalized surfaces. These results indicate that charge is more important than wettability to determine biocompatibility.
authors.affiliation: Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, AV. Prof. Lineu Prestes, 748 São Paulo, SP, Brazil.
authors.forename: Patricia A
authors.initials: PA
authors.lastname: Santos
authors.affiliation: Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, AV. Prof. Lineu Prestes, 748 São Paulo, SP, Brazil.
authors.forename: Cleidiane S
authors.initials: CS
authors.lastname: Rocha
authors.affiliation: Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, AV. Prof. Lineu Prestes, 748 São Paulo, SP, Brazil. Electronic address: baptista@iq.usp.br.
authors.forename: Mauricio S
authors.initials: MS
authors.lastname: Baptista
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry / Q000494:pharmacology; D002448:Cell Adhesion / Q000187:drug effects*; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects*; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects; D006046:Gold / Q000737:chemistry* / Q000494:pharmacology*; D006367:HeLa Cells; D006801:Humans; D016023:Integrins / Q000737:chemistry
chemical_list: D001672:Biocompatible Materials; D016023:Integrins; D006046:Gold
keywords: Biocompatibility; Integrins; Protein adsorption; Self-assembling monolayers; Thiol modifiers; Wettability