title: Hydrophilic interaction chromatography combined with tandem mass spectrometry method for the quantification of tobramycin in human plasma and its application in a pharmacokinetic study.
abstract: A fast, sensitive and specific hydrophilic interaction chromatography combined with tandem mass spectrometry (HILIC-MS/MS) method was developed for the determination of tobramycin in human plasma. With sisomycin as internal standard, the analysis was carried out on a hilic column (150mm×2.1mm, 3.5μm) using a mobile phase consisting of acetonitrile: 5mM ammonium acetate and 0.1% formic acid (60:40, v/v). The detection was performed by tandem spectrometry via electrospray ionization (ESI). Linear calibration curves were obtained in the concentration range of 10.51-1051ng/mL for tobramycin, with a lower limit of quantification of 10.51ng/mL. The intra- and inter-day precision (RSD) values were below 15% and accuracy (RE) was 1.3-5.7% at all QC levels. The method was applicable to the clinical study of the pharmacokinetics of tobramycin in healthy volunteers.
authors.affiliation: Hygiene Detection Center, School of Public Health and Tropical Medicine, Southern Medical University, Guangzhou 510515, China. Electronic address: yun-niu@163.com.
authors.forename: Lingyun
authors.initials: L
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Organic Geochemistry, Guangzhou Institute of Geochemistry, Chinese Academy of Science, Guangzhou 510640, China.
authors.forename: Huashan
authors.initials: H
authors.lastname: Chen
authors.affiliation: Hygiene Detection Center, School of Public Health and Tropical Medicine, Southern Medical University, Guangzhou 510515, China.
authors.forename: Mei
authors.initials: M
authors.lastname: Shen
keywords: HILIC-MS/MS; Human plasma; Pharmacokinetics; Tandem mass spectrometry; Tobramycin