title: A screening methodology for purifying proteins with aggregation problems.
abstract: Many proteins are prone to aggregate or insoluble for different reasons. This poses an extraordinary challenge at the expression level, but even more during downstream purification processes. Here we describe a strategy that we developed for purifying prone-to-aggregate proteins. Our methodology can be easily implemented in small laboratories without the need for automated, expensive platforms. This procedure is especially suitable for intrinsically disordered proteins (IDPs) and for proteins with intrinsically disordered regions (IDRs). Such proteins are likely to aggregate due to their lack of tertiary structure and their extended and flexible conformations. Similar methodologies can be applied to other proteins with comparable tendency to aggregate during the expression or purification steps. In this chapter, we will mainly focus on protein solubility and stability issues during purification and storage, on factors that can prevent aggregation or maintain solubility, and on the importance of the early elimination of aggregates during protein purification.
authors.affiliation: Protein Purification Facility, Wolfson Centre for Applied Structural Biology, The Hebrew University of Jerusalem, Safra Campus, Givat Ram, Jerusalem, 91904, Israel, mario.l@mail.huji.ac.il.
authors.forename: Mario
authors.initials: M
authors.lastname: Lebendiker
authors.forename: Michal
authors.initials: M
authors.lastname: Maes
authors.forename: Assaf
authors.initials: A
authors.lastname: Friedler
mesh_terms: D000818:Animals; D006801:Humans; D064267:Intrinsically Disordered Proteins / Q000302:isolation & purification* / Q000378:metabolism; D066329:Protein Aggregates / Q000502:physiology*; D011487:Protein Conformation; D055550:Protein Stability; D012995:Solubility
chemical_list: D064267:Intrinsically Disordered Proteins; D066329:Protein Aggregates