title: Activation of PmRelish from Penaeus monodon by yellow head virus.
abstract: Humoral innate immune response against pathogenic infection is partly responsible by the Imd pathway in which a transcription factor Relish relays the infection signals to the nuclei for the expression of antimicrobial proteins. A PmRelish gene which encoded a protein of 1195 amino acids was cloned. The PmRelish was constitutively expressed in all tissues tested and mostly up-regulated upon YHV infection. In hemocytes, the PmRelish expression was up-regulated upon Vibrio harveyi, yellow head virus (YHV) and white spot syndrome virus (WSSV) challenges. Using dsRNA silencing of PmRelish gene, it was shown that the expression of penaeidin5 but not anti-lipopolysaccharide factor ALFPm3, crustinPm1 and penaeidin3 was under the regulation of Imd pathway. Under PmRelish silencing, the shrimp were more susceptible to infection by YHV with the 50% survival rate reduced from about 72 h to 42 h. The PmRelish was detected in the cytoplasm of all the hemocytes from both uninfected and YHV-infected shrimp. The accumulation of activated PmRelish in the nuclei was not clearly observed but the activated PmRelish was detected in the YHV-infected hemocytes by Western blot analysis. Thus, the PmRelish and, hence, the Imd pathway respond to the YHV infection.
authors.affiliation: Center of Excellence for Molecular Biology and Genomics of Shrimp, Department of Biochemistry, Faculty of Science, Chulalongkorn University, Phyathai Road, Bangkok 10330, Thailand.
authors.forename: Suwattana
authors.initials: S
authors.lastname: Visetnan
authors.affiliation: Center of Excellence for Molecular Biology and Genomics of Shrimp, Department of Biochemistry, Faculty of Science, Chulalongkorn University, Phyathai Road, Bangkok 10330, Thailand; National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), Pathum Thani 10120, Thailand.
authors.forename: Premruethai
authors.initials: P
authors.lastname: Supungul
authors.affiliation: Laboratory of Genome Science, Tokyo University of Marine Science and Technology, Konan 4-5-7, Minato, Tokyo 108-8477, Japan.
authors.forename: Ikuo
authors.initials: I
authors.lastname: Hirono
authors.affiliation: Center of Excellence for Molecular Biology and Genomics of Shrimp, Department of Biochemistry, Faculty of Science, Chulalongkorn University, Phyathai Road, Bangkok 10330, Thailand.
authors.forename: Anchalee
authors.initials: A
authors.lastname: Tassanakajon
authors.affiliation: Center of Excellence for Molecular Biology and Genomics of Shrimp, Department of Biochemistry, Faculty of Science, Chulalongkorn University, Phyathai Road, Bangkok 10330, Thailand. Electronic address: kpvr2@yahoo.com.
authors.forename: Vichien
authors.initials: V
authors.lastname: Rimphanitchayakit
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D060829:Arthropod Proteins / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D015153:Blotting, Western; D006434:Hemocytes / Q000378:metabolism; D008969:Molecular Sequence Data; D009928:Organ Specificity; D033561:Penaeidae / Q000235:genetics* / Q000378:metabolism / Q000821:virology*; D034622:RNA Interference; D012330:RNA, Double-Stranded / Q000378:metabolism; D046828:Roniviridae / Q000502:physiology*; D014157:Transcription Factors; D014733:Vibrio / Q000502:physiology*; D046848:White spot syndrome virus 1 / Q000502:physiology*
chemical_list: D060829:Arthropod Proteins; D012330:RNA, Double-Stranded; D014157:Transcription Factors
keywords: Antimicrobial proteins; Imd pathway; Penaeus monodon; Relish; Yellow head virus