title: Cell cycle transcription factor E2F2 mediates non-stress temperature response of AtHSP70-4 in Arabidopsis.
abstract: AtHSP70 expression exhibits both stress and non-stress temperature response, however, the molecular mechanisms underlying these temperature signaling pathways remain elusive. Here we performed truncation and deletion assay to investigate the cis-elements within the promoter region of AtHSP70-4 (AT3G12580). And found the region between -1000 and -1100 bp from the translation initiation site (TIS) was indispensable for the non-stress temperature response of AtHSP70. Further deletion assay of several candidate motifs within this region suggested that one 'GCGCCAAA' sequence played the critical role. This motif was found as the reverse DNA-binding motif of cell cycle transcription factor E2F family. EMSA assay verified one number of Arabidopsis E2F family-E2F2 could bind to AtHSP70-4 promoter via this motif. These results indicated the temperature regulated expression of AtHSP70-4 may be mediated by cell cycle transcription factors and participate in plant acclimations to non-stress temperature changes.
authors.affiliation: Lab of Plant Cell Biology, Shanghai Key Laboratory of Bio-Energy Crops, School of Life Sciences, Shanghai University, Shanghai 200444, China.
authors.forename: Shumin
authors.initials: S
authors.lastname: Zhou
authors.affiliation: Lab of Plant Cell Biology, Shanghai Key Laboratory of Bio-Energy Crops, School of Life Sciences, Shanghai University, Shanghai 200444, China.
authors.forename: Hengji
authors.initials: H
authors.lastname: Sun
authors.affiliation: Lab of Plant Cell Biology, Shanghai Key Laboratory of Bio-Energy Crops, School of Life Sciences, Shanghai University, Shanghai 200444, China.
authors.forename: Bang
authors.initials: B
authors.lastname: Zheng
authors.affiliation: Lab of Plant Cell Biology, Shanghai Key Laboratory of Bio-Energy Crops, School of Life Sciences, Shanghai University, Shanghai 200444, China.
authors.forename: Ruisha
authors.initials: R
authors.lastname: Li
authors.affiliation: Lab of Plant Cell Biology, Shanghai Key Laboratory of Bio-Energy Crops, School of Life Sciences, Shanghai University, Shanghai 200444, China. Electronic address: zhw62207@shu.edu.cn.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
mesh_terms: D017360:Arabidopsis / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000378:metabolism*; D001483:Base Sequence; D002453:Cell Cycle; D004261:DNA Replication; D050703:E2F2 Transcription Factor / Q000378:metabolism*; D017353:Gene Deletion; D018506:Gene Expression Regulation, Plant; D005982:Glutathione Transferase / Q000378:metabolism; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D013696:Temperature
chemical_list: D029681:Arabidopsis Proteins; D050703:E2F2 Transcription Factor; D018840:HSP70 Heat-Shock Proteins; C000595079:HSP70-4 protein, Arabidopsis; D005982:Glutathione Transferase
keywords: Arabidopsis thaliana; AtHSP70-4 promoter; E2F2; Temperature sensitive; cis-Element