title: Application of benzo[a]phenoxazinium chlorides in Antimicrobial Photodynamic Therapy of Candida albicans biofilms.
abstract: The use of Antimicrobial Photodynamic Therapy (APDT) as a new approach to treat localized Candida infections is an emerging and promising field nowadays. The aim of this study was to verify the efficacy of photodynamic therapy using two new benzo[a]phenoxazinium photosensitizers against Candida albicans biofilms: N-(5-(3-hydroxypropylamino)-10-methyl-9H-benzo[a]phenoxazin-9-ylidene)ethanaminium chloride (FSc) and N-(5-(11-hydroxyundecylamino)-10-methyl-9H-benzo[a]phenoxazin-9-ylidene)ethanaminium chloride (FSd). The photodynamic activity of dyes against C. albicans biofilms was evaluated by incubating biofilms with dyes in the range of 100-300 μM for 3 or 18 h followed by illumination at 12 or 36 J cm(-2), using a xenon arc lamp (600 ± 2 nm). A total photoinactivation of C. albicans biofilm cells was achieved using 300 μM of FSc with 18 h of incubation, followed by illumination at 36 J cm(-2). Contrarily, FSd had insignificant effect on biofilms inactivation by APDT. The higher uptake of FSc than FSd dye by biofilms during the dark incubation may explain the greater photodynamic effectiveness achieved with FSc. The results obtained stresses out the FSc-mediated APDT potential use to treat C. albicans infections.
authors.affiliation: Centre of Biological Engineering, University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal.
authors.forename: Marisa
authors.initials: M
authors.lastname: Lopes
authors.affiliation: Centre of Biological Engineering, University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal.
authors.forename: Carlos Tiago
authors.initials: CT
authors.lastname: Alves
authors.affiliation: Centre of Chemistry, University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal; Centre of Physics, University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal.
authors.forename: B
authors.initials: B
authors.lastname: Rama Raju
authors.affiliation: Centre of Chemistry, University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal.
authors.forename: M Sameiro T
authors.initials: MS
authors.lastname: Gonçalves
authors.affiliation: Centre of Physics, University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal.
authors.forename: Paulo J G
authors.initials: PJ
authors.lastname: Coutinho
authors.affiliation: Centre of Biological Engineering, University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal.
authors.forename: Mariana
authors.initials: M
authors.lastname: Henriques
authors.affiliation: Centre of Biological Engineering, University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal. Electronic address: ibelo@deb.uminho.pt.
authors.forename: Isabel
authors.initials: I
authors.lastname: Belo
mesh_terms: D000890:Anti-Infective Agents / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D018441:Biofilms / Q000187:drug effects* / Q000528:radiation effects; D002176:Candida albicans / Q000502:physiology*; D004396:Coloring Agents / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D008027:Light; D008826:Microbial Sensitivity Tests; D008856:Microscopy, Fluorescence; D010078:Oxazines / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D017319:Photosensitizing Agents / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*
chemical_list: D000890:Anti-Infective Agents; D004396:Coloring Agents; D010078:Oxazines; D017319:Photosensitizing Agents
keywords: Antimicrobial photodynamic therapy; Benzo[a]phenoxazines; Biofilms; Candida albicans