title: Prediction of skin permeation by chemical compounds using the artificial membrane, Strat-M™.
abstract: OBJECTIVE
  The usefulness of the synthetic membrane, Strat-M™ as an alternative to human and animal skins was evaluated by estimating the skin permeabilities of chemical compounds.
  
  
  METHODS
  Thirteen chemical compounds with molecular weights (M.W.) of 152-289 and lipophilicities (log Ko/w) of -0.9 to 3.5 were selected. Strat-M™, excised human skin, or hairless rat skin was set in a Franz-type diffusion cell and a saturated solution of each chemical compound was applied to determine membrane permeation profiles. The obtained permeability coefficients (log P) were compared among these membranes.
  
  
  CONCLUSIONS
  Elevations were observed in log P for Strat-M™ with an increase in the log Ko/w of the applied compounds, and similar results were observed with the human and hairless rat skins. A correlation was obtained in log P values between Strat-M™ and human or hairless rat skin. Furthermore, the diffusion and partition parameters of chemicals in Strat-M™ were similar to those in the excised human and rat skins. These results suggest that Strat-M™ could be used as an alternative to animal or human skin in permeation studies.
authors.affiliation: Faculty of Pharmaceutical Sciences, Josai University, 1-1 Keyakidai, Sakado, Saitama 350-0295, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Uchida
authors.affiliation: Faculty of Pharmaceutical Sciences, Josai University, 1-1 Keyakidai, Sakado, Saitama 350-0295, Japan.
authors.forename: Wesam R
authors.initials: WR
authors.lastname: Kadhum
authors.affiliation: Faculty of Pharmaceutical Sciences, Josai University, 1-1 Keyakidai, Sakado, Saitama 350-0295, Japan.
authors.forename: Sayumi
authors.initials: S
authors.lastname: Kanai
authors.affiliation: Faculty of Pharmaceutical Sciences, Josai University, 1-1 Keyakidai, Sakado, Saitama 350-0295, Japan.
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Todo
authors.affiliation: Faculty of Pharmaceutical Sciences, Josai University, 1-1 Keyakidai, Sakado, Saitama 350-0295, Japan.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Oshizaka
authors.affiliation: Faculty of Pharmaceutical Sciences, Josai University, 1-1 Keyakidai, Sakado, Saitama 350-0295, Japan. Electronic address: sugib@josai.ac.jp.
authors.forename: Kenji
authors.initials: K
authors.lastname: Sugibayashi
mesh_terms: D020003:1-Octanol / Q000737:chemistry; D000818:Animals; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D008567:Membranes, Artificial; D008875:Middle Aged; D004364:Pharmaceutical Preparations / Q000378:metabolism; D054772:Rats, Hairless; D012867:Skin / Q000378:metabolism; D012869:Skin Absorption; D014867:Water / Q000737:chemistry
chemical_list: D008567:Membranes, Artificial; D004364:Pharmaceutical Preparations; D014867:Water; D020003:1-Octanol
keywords: Alternative membrane; Membrane permeation; Skin; Strat-M™; Synthetic membrane