title: A simplified method for simultaneous detection of Rice stripe virus and Rice black-streaked dwarf virus in insect vector.
abstract: Rice stripe virus (RSV) and Rice black-streaked dwarf virus (RBSDV) are transmitted by their common vector small brown planthopper (SBPH) that cause serious crop losses in China. A simple reverse transcription-PCR method was developed for the simultaneous detection of RSV and RBSDV in single SBPH. Three primers targeted to RSV-RNA4 and RBSDV-S2 segments were designed to amplify respectively 1114-bp and 414-bp fragments in a reaction. The method is reliable, rapid and inexpensive for detecting the two viruses in vector, which could facilitate better forecasting and control of the virus diseases. Using this method, it was found that SBPH could carry RSV and RBSDV simultaneously.
authors.affiliation: Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Jiangsu Technical Service Center of Diagnosis and Detection for Plant Virus Diseases, Nanjing 210014, China.
authors.forename: Shuo
authors.initials: S
authors.lastname: Li
authors.affiliation: Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Jiangsu Technical Service Center of Diagnosis and Detection for Plant Virus Diseases, Nanjing 210014, China; College of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225009, China.
authors.forename: Xi
authors.initials: X
authors.lastname: Wang
authors.affiliation: College of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225009, China.
authors.forename: Jianxiang
authors.initials: J
authors.lastname: Xu
authors.affiliation: Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Jiangsu Technical Service Center of Diagnosis and Detection for Plant Virus Diseases, Nanjing 210014, China.
authors.forename: Yinghua
authors.initials: Y
authors.lastname: Ji
authors.affiliation: Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Jiangsu Technical Service Center of Diagnosis and Detection for Plant Virus Diseases, Nanjing 210014, China. Electronic address: yjzhou@jaas.ac.cn.
authors.forename: Yijun
authors.initials: Y
authors.lastname: Zhou
mesh_terms: D000818:Animals; D002681:China; D003365:Costs and Cost Analysis; D004772:Entomology / Q000379:methods*; D006430:Hemiptera / Q000821:virology*; D007303:Insect Vectors / Q000821:virology*; D012275:Oryza / Q000821:virology; D010935:Plant Diseases / Q000821:virology; D012087:Reoviridae / Q000302:isolation & purification*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D029364:Tenuivirus / Q000302:isolation & purification*; D013997:Time Factors; D014773:Virology / Q000379:methods*
keywords: Rice black-streaked dwarf virus; Rice stripe virus; Simultaneous detection via RT-PCR