title: Sinomonas mesophila sp. nov., isolated from ancient fort soil.
abstract: A moderate growing strain designated as MPKL 26(T) was isolated from a soil sample of Bidar Fort, Karnataka, India. The strain MPKL 26(T) was Gram positive, bent rod in shape. The optimum pH and temperature for growth was 7.0 and 30 °C, respectively. The 16S ribosomal RNA gene sequence analysis revealed that strain MPKL 26(T) was closely related to S. atrocyanea DSM 20127(T) (98.09%), S. flava CW 108(T) (98.04%), S. soli CW 59(T) (97.99%) and S. notoginsengisoli SYP-B575(T) (97.0%) and showed DNA-DNA hybridization relatedness (46.05±1.2, 33.56±2.55, 32.56±1.7 and 26.79±2.5, respectively, between these strains) less than the threshold value for the delineation of genomic species. The peptidoglycon type was A3α type with glycine, alanine, lysine and glutamic acid as the amino acids. The whole-cell sugars were fructose, ribose, mannose, glucose and galactose. The polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol along with three unknown polar lipids. The fatty acid profile contained C14:0, C16:0, iso-C14:0, iso-C15:0, iso-C16:0, iso-C17:0, anteiso-C15:0, anteiso-C17:0 and summed feature 4 (17:1 iso I/anteiso B). The predominant respiratory quinine was MK-9(H2) with MK-10(H2), MK-8(H2) and MK-8(H4) as minor respiratory quinines. The G+C content of the genomic DNA was 68.8 mol%. On the basis of phenotypic, chemotaxonomic and molecular characteristics, the strain MPKL 26(T) represents a novel species of the genus Sinomonas, for which the name Sinomonas mesophila sp. nov. is proposed with MPKL 26(T) as the type strain (=NCIM 5552(T)= JCM 30094(T)).
authors.affiliation: 1] State Key Laboratory of Biocontrol, Key Laboratory of Biodiversity Dynamics and Conservation of Guangdong Higher Education Institutes, College of Ecology and Evolution, Sun Yat-Sen University, Guangzhou, China [2] Department of Microbiology, Gulbarga University, Gulbarga, India.
authors.forename: Deene Manik
authors.identifier: 0000000247773452
authors.initials: DM
authors.lastname: Prabhu
authors.affiliation: Department of Medical Laboratory Technology, Faculty of Applied Medical Sciences, Northern Border University, Arar, Kingdom of Saudi Arabia.
authors.forename: Syed Raziuddin
authors.initials: SR
authors.lastname: Quadri
authors.affiliation: Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education, Yunnan Institute of Microbiology, Yunnan University, Kunming, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Cheng
authors.affiliation: Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education, Yunnan Institute of Microbiology, Yunnan University, Kunming, China.
authors.forename: Lan
authors.initials: L
authors.lastname: Liu
authors.affiliation: China Tobacco Yunnan Industrial Co., Ltd, Kunming, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Chen
authors.affiliation: China Tobacco Yunnan Industrial Co., Ltd, Kunming, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Department of Zoology, Bioproducts Research Chair (BRC), College of Science, King Saud University, Riyadh, Kingdom of Saudi Arabia.
authors.forename: Wael N
authors.initials: WN
authors.lastname: Hozzein
authors.affiliation: Department of Microbiology, Gulbarga University, Gulbarga, India.
authors.forename: Kattimani
authors.initials: K
authors.lastname: Lingappa
authors.affiliation: 1] State Key Laboratory of Biocontrol, Key Laboratory of Biodiversity Dynamics and Conservation of Guangdong Higher Education Institutes, College of Ecology and Evolution, Sun Yat-Sen University, Guangzhou, China [2] Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education, Yunnan Institute of Microbiology, Yunnan University, Kunming, China.
authors.forename: Wen-Jun
authors.initials: WJ
authors.lastname: Li
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D004269:DNA, Bacterial / Q000235:genetics; D024881:Drug Resistance, Bacterial; D008835:Micrococcaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D010802:Phylogeny; D012988:Soil Microbiology; D013045:Species Specificity
chemical_list: D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial