title: SAMHD1 is down regulated in lung cancer by methylation and inhibits tumor cell proliferation.
abstract: The function of dNTP hydrolase SAMHD1 as a viral restriction factor to inhibit the replication of several viruses in human immune cells was well established. However, its regulation and function in lung cancer have been elusive. Here, we report that SAMHD1 is down regulated both on protein and mRNA levels in lung adenocarcinoma compared to adjacent normal tissue. We also found that SAMHD1 promoter is highly methylated in lung adenocarcinoma, which may inhibit its gene expression. Furthermore, over expression of the SAMHD1 reduces dNTP level and inhibits the proliferation of lung tumor cells. These results reveal the regulation and function of SAMHD1 in lung cancer, which is important for the proliferation of lung tumor cells.
authors.affiliation: Department of Medical Oncology, Fudan University Shanghai Cancer Center, Shanghai 200032, China.
authors.forename: Jia-lei
authors.initials: JL
authors.lastname: Wang
authors.affiliation: Department of Thoracic Surgery, The Huadong Hospital, Fudan University, Shanghai 200040, China.
authors.forename: Fan-zhen
authors.initials: FZ
authors.lastname: Lu
authors.affiliation: Department of Thoracic Surgery, The Huadong Hospital, Fudan University, Shanghai 200040, China. Electronic address: shengxiaoyong_sh@163.com.
authors.forename: Xiao-Yong
authors.initials: XY
authors.lastname: Shen
authors.affiliation: Department of Thoracic Surgery, The Huadong Hospital, Fudan University, Shanghai 200040, China. Electronic address: WuYun_hd@163.com.
authors.forename: Yun
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Department of Thoracic Surgery, The Huadong Hospital, Fudan University, Shanghai 200040, China.
authors.forename: Li-ting
authors.initials: LT
authors.lastname: Zhao
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics / Q000378:metabolism*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D019175:DNA Methylation; D015536:Down-Regulation; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics / Q000378:metabolism*; D020559:Monomeric GTP-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000378:metabolism; D000076106:SAM Domain and HD Domain-Containing Protein 1
chemical_list: D012333:RNA, Messenger; D000076106:SAM Domain and HD Domain-Containing Protein 1; C484280:SAMHD1 protein, human; D020559:Monomeric GTP-Binding Proteins
keywords: Cell proliferation; Lung adenocarcinoma; SAMHD1; dNTP