title: Dual-specificity phosphatase 6 predicts the sensitivity of progestin therapy for atypical endometrial hyperplasia.
abstract: OBJECTIVE
  We previously found that Dual-specificity phosphatase 6 (Dusp6) over-expression enhanced the growth-promoting effect of estrogen in endometrial adenocarcinoma cells. The aim of this study was to explore the correlation of Dusp6 expression with progestin sensitivity in atypical endometrial hyperplasia (AEH) and earlier endometrial carcinomas (EC).
  
  
  METHODS
  Using immunohistochemistry study, we analyzed the expression of Dusp6 protein in AEH.
  
  
  RESULTS
  We found that progestin treatment was effective in 89% of AEH and 50% of EC. Before treatment, Dusp6 expression was significantly higher in progestin-sensitive AEH groups compared with progestin-resistant groups. After treatment, Dusp6 expression was significantly upregulated in progestin-sensitive groups, but not in progestin-resistant groups. Moreover, a high-dose of Dusp6 transfection significantly enhanced progestin-induced growth-inhibition in Ishikawa cells.
  
  
  CONCLUSIONS
  Dusp6 could be a predicting marker for deciding the effectiveness of progestin therapy in AEH.
authors.affiliation: Department of Obstetrics and Gynecology, Shandong Provincial Hospital Affiliated to Shandong University, 324 Jingwu Road, Jinan, Shandong 250021, People's Republic of China.
authors.forename: Hui
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Department of Obstetrics and Gynecology, Shandong Provincial Hospital Affiliated to Shandong University, 324 Jingwu Road, Jinan, Shandong 250021, People's Republic of China.
authors.forename: Lei
authors.initials: L
authors.lastname: Yan
authors.affiliation: Department of Obstetrics and Gynecology, Shandong Provincial Hospital Affiliated to Shandong University, 324 Jingwu Road, Jinan, Shandong 250021, People's Republic of China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Bai
authors.affiliation: Department of Obstetrics and Gynecology, Shandong Provincial Hospital Affiliated to Shandong University, 324 Jingwu Road, Jinan, Shandong 250021, People's Republic of China.
authors.forename: Chunyan
authors.initials: C
authors.lastname: Li
authors.affiliation: Department of Obstetrics and Gynecology, Shandong Provincial Hospital Affiliated to Shandong University, 324 Jingwu Road, Jinan, Shandong 250021, People's Republic of China.
authors.forename: Qiufen
authors.initials: Q
authors.lastname: Guo
authors.affiliation: Department of General Surgery, Shandong Rongjun General Hospital, 23 Jiefang Road, Jinan, Shandong 250013, People's Republic of China.
authors.forename: Chong
authors.initials: C
authors.lastname: Wang
authors.affiliation: Department of Obstetrics and Gynecology, Shandong Provincial Hospital Affiliated to Shandong University, 324 Jingwu Road, Jinan, Shandong 250021, People's Republic of China.
authors.forename: Xingbo
authors.initials: X
authors.lastname: Zhao
authors.affiliation: Department of Obstetrics and Gynecology, Shandong Provincial Hospital Affiliated to Shandong University, 324 Jingwu Road, Jinan, Shandong 250021, People's Republic of China. Electronic address: mingjiangli1963@gmail.com.
authors.forename: Mingjiang
authors.initials: M
authors.lastname: Li
mesh_terms: D018931:Antineoplastic Agents, Hormonal / Q000627:therapeutic use*; D015415:Biomarkers / Q000378:metabolism; D045744:Cell Line, Tumor; D019008:Drug Resistance, Neoplasm; D054642:Dual Specificity Phosphatase 6 / Q000378:metabolism*; D004714:Endometrial Hyperplasia / Q000188:drug therapy* / Q000201:enzymology; D016889:Endometrial Neoplasms / Q000188:drug therapy* / Q000201:enzymology; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D017258:Medroxyprogesterone Acetate / Q000627:therapeutic use*; D011230:Precancerous Conditions / Q000188:drug therapy* / Q000201:enzymology; D011372:Progestins / Q000627:therapeutic use*; D016896:Treatment Outcome; D015854:Up-Regulation
chemical_list: D018931:Antineoplastic Agents, Hormonal; D015415:Biomarkers; D011372:Progestins; D017258:Medroxyprogesterone Acetate; C517035:DUSP6 protein, human; D054642:Dual Specificity Phosphatase 6
keywords: Atypical endometrial hyperplasia; Dusp6; Progesterone resistance; Progesterone sensitivity