title: Detection of embryonic stem cell lysate biomarkers by surface plasmon resonance with reduced nonspecific adsorption.
abstract: Surface plasmon resonance imaging (SPRi) has emerged as a versatile biosensor to detect a wide range of biomolecular interactions with divergent potential applications. However, the use of this advanced-level technology for stem cell lysate study is still not much explored. Cell lysates are significant biological analytes used for disease diagnostics and proteomic studies, but their complex nature limits their use as an analyte for SPRi biosensors. Here, we review the problems associated with the use of SPRi for stem cell lysate study and examine the role of surface chemistry, running buffer, and blocking solution in order to minimize nonspecific adsorption (NSA). We detect the expression of Oct4, Sox2, Nanog, Rex1, and Lin28 biomarkers present in mouse embryonic stem cell (mESC) lysate against their corresponding antibodies immobilized on the sensor surface with reduced NSA. The current study shows that the conjunction of SPRi and microarray can be used as a label-free, high-throughput, and rapid technique for detection of biomarkers and their relative abundance in stem cell lysate study.
authors.affiliation: National Center for Nanoscience and Technology, Beijing 100190, China; University of Chinese Academy of Sciences, Beijing 100049, China.
authors.forename: Deependra
authors.initials: D
authors.lastname: Tyagi
authors.affiliation: National Center for Nanoscience and Technology, Beijing 100190, China; University of Chinese Academy of Sciences, Beijing 100049, China.
authors.forename: Javier Batista
authors.initials: JB
authors.lastname: Perez
authors.affiliation: National Center for Nanoscience and Technology, Beijing 100190, China; University of Chinese Academy of Sciences, Beijing 100049, China.
authors.forename: Amita
authors.initials: A
authors.lastname: Nand
authors.affiliation: National Center for Nanoscience and Technology, Beijing 100190, China; University of Chinese Academy of Sciences, Beijing 100049, China; Department of Chemistry, Tsinghua University, Beijing 100084, China.
authors.forename: Cheng
authors.initials: C
authors.lastname: Zhiqiang
authors.affiliation: Centre for Stem Cell Biology and Regenerative Medicine, School of Medicine, Tsinghua University, Beijing 100084, China.
authors.forename: Peizhe
authors.initials: P
authors.lastname: Wang
authors.affiliation: Centre for Stem Cell Biology and Regenerative Medicine, School of Medicine, Tsinghua University, Beijing 100084, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Na
authors.affiliation: National Center for Nanoscience and Technology, Beijing 100190, China; University of Chinese Academy of Sciences, Beijing 100049, China. Electronic address: jizhu@nanoctr.cn.
authors.forename: Jingsong
authors.initials: J
authors.lastname: Zhu
mesh_terms: D000327:Adsorption; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D002021:Buffers; D016923:Cell Death; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000378:metabolism*; D005786:Gene Expression Regulation; D051379:Mice; D020349:Surface Plasmon Resonance / Q000379:methods*; D013997:Time Factors; D046888:Tissue Array Analysis
chemical_list: D015415:Biomarkers; D002021:Buffers
keywords: Antibody array; Nonspecific adsorption; SPRi; Stem cell