title: Localization of dendritic cells in the gut epithelium requires MAdCAM-1.
abstract: Directed migration of immune cells is a prerequisite for immune responses. T and B cell migration to the gut is secured by interaction of mucosal addressin cell-adhesion molecule-1 (MAdCAM-1) and β7 integrin. Here we report a novel function for MAdCAM-1: that of mediating intestinal localization of dendritic cells (DCs). In homeostasis, both MAdCAM-1-deficient and β7 integrin-deficient mice exhibit a reduced frequency of CD11c(+) cells, including CD103(+) DCs and plasmacytoid DCs (pDCs), in the gut epithelium. Deficiency of either MAdCAM-1 or β7 integrin reduces the migration efficiency of pDCs into the intestinal intraepithelial (IE) compartment. Both mouse strains display a decreased migration efficiency of precursors for conventional DCs (cDCs), from the circulation into the epithelium. By contrast, the migration of activated DCs from the small intestine to MLN is unchanged in MAdCAM-1-deficient mice. These findings suggest that MAdCAM-1 is important for the β7 integrin-dependent intestinal localization of both cDCs and pDCs.
authors.affiliation: Department of Pediatrics, Medical Faculty, RWTH Aachen University, D-52074 Aachen, Germany.
authors.forename: Thomas
authors.initials: T
authors.lastname: Clahsen
authors.affiliation: Institute of Immunology, Hannover Medical School, D-30625 Hannover, Germany.
authors.forename: Oliver
authors.initials: O
authors.lastname: Pabst
authors.affiliation: Department of Pediatrics, Medical Faculty, RWTH Aachen University, D-52074 Aachen, Germany.
authors.forename: Klaus
authors.initials: K
authors.lastname: Tenbrock
authors.affiliation: Department of Pediatrics, Medical Faculty, RWTH Aachen University, D-52074 Aachen, Germany. Electronic address: anschippers@ukaachen.de.
authors.forename: Angela
authors.initials: A
authors.lastname: Schippers
authors.affiliation: Department of Pediatrics, Medical Faculty, RWTH Aachen University, D-52074 Aachen, Germany. Electronic address: nwagner@ukaachen.de.
authors.forename: Norbert
authors.initials: N
authors.lastname: Wagner
mesh_terms: D000818:Animals; D015815:Cell Adhesion Molecules / Q000235:genetics / Q000378:metabolism*; D002465:Cell Movement; D003713:Dendritic Cells / Q000166:cytology / Q000378:metabolism*; D005434:Flow Cytometry; D007413:Intestinal Mucosa / Q000166:cytology / Q000378:metabolism*; D051379:Mice; D009088:Mucoproteins
chemical_list: D015815:Cell Adhesion Molecules; C489082:Madcam1 protein, mouse; D009088:Mucoproteins
keywords: Dendritic cells;; MAdCAM-1;; Migration;; β7 integrin