title: Purification and identification of dipeptidyl peptidase (DPP) IV inhibitory peptides from the macroalga Palmaria palmata.
abstract: Dipeptidyl peptidase (DPP)-IV inhibitory peptides were purified and identified from an aqueous Palmaria palmata protein extract hydrolysed with Corolase PP. The hydrolysate was fractionated by solid phase extraction (SPE) using a C18 matrix followed by semi-preparative reverse phase-high performance liquid chromatography (SP RP-HPLC). IC50 values of 1.47 ± 0.09, 0.54 ± 0.03 and 0.36 ± 0.03 mg/ml were obtained for the hydrolysate, the 25%--acetonitrile (ACN) SPE fraction and the most active SP RP-HPLC peptide fraction (SP RP-HPLC 25_F28), respectively. Thirteen peptide sequences were identified following UPLC-ESI MS/MS analysis of SP RP-HPLC 25_F28. Three novel DPP-IV inhibitory peptides, Ile-Leu-Ala-Pro, Leu-Leu-Ala-Pro and Met-Ala-Gly-Val-Asp-His-Ile, with IC50 values in the range 43-159 μM were identified. The results indicate that P. palmata derived peptides may have potential as functional food ingredients in the prevention and management of type 2 diabetes.
authors.affiliation: Department of Life Sciences, University of Limerick, Limerick, Ireland.
authors.forename: Pádraigín A
authors.initials: PA
authors.lastname: Harnedy
authors.affiliation: Department of Life Sciences, University of Limerick, Limerick, Ireland.
authors.forename: Martina B
authors.initials: MB
authors.lastname: O'Keeffe
authors.affiliation: Department of Life Sciences, University of Limerick, Limerick, Ireland. Electronic address: dick.fitzgerald@ul.ie.
authors.forename: Richard J
authors.initials: RJ
authors.lastname: FitzGerald
mesh_terms: D000595:Amino Acid Sequence; D002851:Chromatography, High Pressure Liquid; D056148:Chromatography, Reverse-Phase; D018819:Dipeptidyl Peptidase 4 / Q000737:chemistry / Q000378:metabolism; D054873:Dipeptidyl-Peptidase IV Inhibitors / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D020128:Inhibitory Concentration 50; D010455:Peptides / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D000461:Rhodophyta / Q000378:metabolism*; D052616:Solid Phase Extraction; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D054873:Dipeptidyl-Peptidase IV Inhibitors; D010455:Peptides; D018819:Dipeptidyl Peptidase 4
keywords: Bioactive peptide; Dipeptidyl peptidase IV; Palmaria palmata; Type 2 diabetes