title: Can your protein be sumoylated? A quick summary and important tips to study SUMO-modified proteins.
abstract: A diverse set of SUMO target proteins has been identified. Therefore, there is a growing interest in studying sumoylation and SUMO interactions in cells. When the sumoylation of a protein or a SUMO interaction is suspected, a standard co-immunoprecipitation analysis using anti-SUMO and anti-target protein antibody is usually performed as a first step. However, the identification of endogenous sumoylated proteins is challenging because of the activity of isopeptidases, and often only a small fraction of a target protein is sumoylated at a given time. Here, we briefly summarize several important steps to ensure a successful co-immunoprecipitation analysis to detect possible sumoylation.
authors.affiliation: Department of Biology, Yeshiva University, New York, NY 10016, USA.
authors.forename: Yuxuan
authors.initials: Y
authors.lastname: Xiao
authors.affiliation: Department of Biology, Yeshiva University, New York, NY 10016, USA.
authors.forename: Daniel
authors.initials: D
authors.lastname: Pollack
authors.affiliation: Laboratory for Macromolecular Analysis and Proteomics, Albert Einstein College of Medicine, Bronx, NY 10461, USA.
authors.forename: Edward
authors.initials: E
authors.lastname: Nieves
authors.affiliation: Department of Biology, Yeshiva University, New York, NY 10016, USA.
authors.forename: Aby
authors.initials: A
authors.lastname: Winchell
authors.affiliation: Laboratory for Macromolecular Analysis and Proteomics, Albert Einstein College of Medicine, Bronx, NY 10461, USA.
authors.forename: Myrasol
authors.initials: M
authors.lastname: Callaway
authors.affiliation: Department of Biology, Yeshiva University, New York, NY 10016, USA; Department of Developmental and Molecular Biology, Albert Einstein College of Medicine, Bronx, NY 10461, USA. Electronic address: vigodner@yu.edu.
authors.forename: Margarita
authors.initials: M
authors.lastname: Vigodner
mesh_terms: D047468:Immunoprecipitation; D025941:Protein Interaction Mapping / Q000379:methods*; D025841:Small Ubiquitin-Related Modifier Proteins / Q000378:metabolism*; D058207:Sumoylation
chemical_list: D025841:Small Ubiquitin-Related Modifier Proteins
keywords: Co-immunoprecipitation; In vitro sumoylation; Isopeptidases; Mass spectrometry; SUMO; Sumoylation