title: Design and application of a novel ionic liquid with the property of strengthening coenzyme regeneration for whole-cell bioreduction in an ionic liquid-distilled water medium.
abstract: Focusing on the task-specific properties of ionic liquids (ILs), a novel IL was designed by combining the quaternary ammonium cationic surfactant, [N1,1,1,1](+), with benign amino acid anion ([Cys](-)), and was successfully employed in whole-cell-catalyzed bioreduction by Trichodermaasperellum ZJPH0810 using an ionic liquid-distilled water medium. As expected, based on better understanding about the effects of ILs' characteristics of cations and anions on T. asperellum ZJPH0810-catalyzed bioreduction and the optimization of reaction parameters, the developed tetramethylammonium cysteine ([N1,1,1,1][Cys])-containing system is more efficient for the asymmetric reduction of 3,5-bis(trifluoromethyl) acetophenone to (R)-[3,5-bis(trifluoromethyl)phenyl] ethanol by whole-cell catalysis in contrast to that in monophasic aqueous system. Herein, a new biotechnological application for designed type of ILs is proposed due to its novel property of strengthening coenzyme regeneration during the bioreduction process. The designed IL [N1,1,1,1][Cys] was also evaluated in Candidatropicalis 104-catalyzed bioreduction to board its application, with remarkable success.
authors.affiliation: College of Pharmaceutical Science, Zhejiang University of Technology, Hangzhou 310032, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Li
authors.affiliation: College of Pharmaceutical Science, Zhejiang University of Technology, Hangzhou 310032, China. Electronic address: wangpu@zjut.edu.cn.
authors.forename: Pu
authors.initials: P
authors.lastname: Wang
authors.affiliation: College of Pharmaceutical Science, Zhejiang University of Technology, Hangzhou 310032, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Huang
authors.affiliation: College of Pharmaceutical Science, Zhejiang University of Technology, Hangzhou 310032, China.
authors.forename: Jia
authors.initials: J
authors.lastname: Sun
mesh_terms: D019149:Bioreactors / Q000382:microbiology*; D002384:Catalysis; D003067:Coenzymes / Q000737:chemistry; D052578:Ionic Liquids / Q000737:chemistry*; D014242:Trichoderma; D014867:Water / Q000737:chemistry
chemical_list: D003067:Coenzymes; D052578:Ionic Liquids; D014867:Water
keywords: Asymmetric biocatalytic reduction; Chiral alcohol; Coenzyme regeneration; Tetramethylammonium cysteine ([N1,1,1,1][Cys])-containing system