title: Tough and elastic hydrogel of hyaluronic acid and chondroitin sulfate as potential cell scaffold materials.
abstract: Natural polysaccharides are extensively investigated as cell scaffold materials for cellular adhesion, proliferation, and differentiation due to their excellent biocompatibility, biodegradability, and biofunctions. However, their application is often severely limited by their mechanical behavior. In this study, a tough and elastic hydrogel scaffold was prepared with hyaluronic acid (HA) and chondroitin sulfate (CS). HA and CS were conjugated with tyramine (TA) and the degree of substitution (DS) was 10.7% and 11.3%, respectively, as calculated by (1)H NMR spectra. The hydrogel was prepared by mixing HA-TA and CS-TA in presence of H2O2 and HRP. The sectional morphology of hydrogels was observed by SEM, static and dynamic mechanical properties were analyzed by Shimadzu electromechanical testing machine and dynamic mechanical thermal analyzer Q800. All samples showed good ability to recover their appearances after deformation, the storage modulus (E') of hydrogels became higher as the testing frequency went up. Hydrogels also showed fatigue resistance to cyclic compression. Mesenchymal stem cells encapsulated in hydrogels showed good cell viability as detected by CLSM. This study suggests that the hydrogels have both good mechanical properties and biocompatibility, and may serve as model systems to explore mechanisms of deformation and energy dissipation or find some applications in tissue engineering.
authors.affiliation: National Engineering Research Center for Biomaterials, Sichuan University, Wangjiang Road 29, Chengdu 610064, China.
authors.forename: Yilu
authors.initials: Y
authors.lastname: Ni
authors.affiliation: National Engineering Research Center for Biomaterials, Sichuan University, Wangjiang Road 29, Chengdu 610064, China.
authors.forename: Zhurong
authors.initials: Z
authors.lastname: Tang
authors.affiliation: National Engineering Research Center for Biomaterials, Sichuan University, Wangjiang Road 29, Chengdu 610064, China.
authors.forename: Wanxu
authors.initials: W
authors.lastname: Cao
authors.affiliation: National Engineering Research Center for Biomaterials, Sichuan University, Wangjiang Road 29, Chengdu 610064, China.
authors.forename: Hai
authors.initials: H
authors.lastname: Lin
authors.affiliation: National Engineering Research Center for Biomaterials, Sichuan University, Wangjiang Road 29, Chengdu 610064, China.
authors.forename: Yujiang
authors.initials: Y
authors.lastname: Fan
authors.affiliation: National Engineering Research Center for Biomaterials, Sichuan University, Wangjiang Road 29, Chengdu 610064, China. Electronic address: lkgchem@hotmail.com.
authors.forename: Likun
authors.initials: L
authors.lastname: Guo
authors.affiliation: National Engineering Research Center for Biomaterials, Sichuan University, Wangjiang Road 29, Chengdu 610064, China. Electronic address: Xingdongzhang@hotmail.com.
authors.forename: Xingdong
authors.initials: X
authors.lastname: Zhang
mesh_terms: D000818:Animals; D001672:Biocompatible Materials; D049109:Cell Proliferation; D002470:Cell Survival; D002478:Cells, Cultured; D002809:Chondroitin Sulfates / Q000737:chemistry*; D006820:Hyaluronic Acid / Q000737:chemistry*; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry*; D008422:Materials Testing; D055595:Mechanical Phenomena; D059630:Mesenchymal Stem Cells; D019906:Nuclear Magnetic Resonance, Biomolecular; D011817:Rabbits; D023822:Tissue Engineering; D054457:Tissue Scaffolds
chemical_list: D001672:Biocompatible Materials; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D006820:Hyaluronic Acid; D002809:Chondroitin Sulfates
keywords: Chondroitin sulfate; Hyaluronic acid; Hybrid hydrogels