title: High-level fluoroquinolone resistant Salmonella enterica serovar Kentucky ST198 epidemic clone with IncA/C conjugative plasmid carrying bla(CTX-M-25) gene.
abstract: Multidrug resistant Salmonella Kentucky strains have been isolated from turkeys in Poland since 2009. Multiple mutations within chromosomal genes gyrA and parC were responsible for high-level ciprofloxacin resistance. One of the isolates was extended spectrum β-lactamase- (ESBL) positive: the strain 1643/2010 carried a conjugative 167,779 bps plasmid of IncA/C family. The sequence analysis revealed that it carried a blaCTX-M-25 gene and an integron with another β-lactamase encoding gene-blaOXA-21. This is the first known report of a CTX-M-25 encoding gene both in Poland and in Salmonella Kentucky world-wide, as well as in the IncA/C plasmid. Analysis of the integron showed a novel arrangement of gene cassettes-aacA4, aacC-A1 and blaOXA-21 where the latter might result from an intergeneric gene transfer. The study confirmed Salmonella Kentucky population isolated in Poland belongs to global epidemics of high level fluoroquinolone resistant clone ST198 that can carry rare β-lactamase genes.
authors.affiliation: Department of Microbiology, National Veterinary Research Institute, Partyzantów 57, 24-100 Puławy, Poland. Electronic address: wasyl@piwet.pulawy.pl.
authors.forename: Dariusz
authors.initials: D
authors.lastname: Wasyl
authors.affiliation: Department of Microbial Biochemistry, Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawińskiego 5A, 02-106 Warsaw, Poland.
authors.forename: Izabela
authors.initials: I
authors.lastname: Kern-Zdanowicz
authors.affiliation: Department of Poultry Diseases, National Veterinary Research Institute, Partyzantów 57, 24-100 Puławy, Poland.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Domańska-Blicharz
authors.affiliation: Department of Microbiology, National Veterinary Research Institute, Partyzantów 57, 24-100 Puławy, Poland.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Zając
authors.affiliation: Department of Microbiology, National Veterinary Research Institute, Partyzantów 57, 24-100 Puławy, Poland.
authors.forename: Andrzej
authors.initials: A
authors.lastname: Hoszowski
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001483:Base Sequence; D002939:Ciprofloxacin / Q000494:pharmacology; D024901:Drug Resistance, Multiple, Bacterial; D024841:Fluoroquinolones / Q000494:pharmacology; D032023:Integrons / Q000235:genetics; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics; D011044:Poland / Q000453:epidemiology; D011201:Poultry Diseases / Q000453:epidemiology* / Q000382:microbiology; D012481:Salmonella Infections, Animal / Q000453:epidemiology* / Q000382:microbiology; D019779:Salmonella enterica / Q000187:drug effects / Q000235:genetics*; D017422:Sequence Analysis, DNA; D065288:Serogroup; D014422:Turkeys / Q000382:microbiology*; D001618:beta-Lactamases / Q000235:genetics*
chemical_list: D000900:Anti-Bacterial Agents; D024841:Fluoroquinolones; D002939:Ciprofloxacin; D001618:beta-Lactamases
keywords: Extended spectrum beta lactamases (ESBLs); IncA/C; Plasmid-mediated resistance; Quinolone resistance