title: Global proteome turnover analyses of the Yeasts S. cerevisiae and S. pombe.
abstract: How cells maintain specific levels of each protein and whether that control is evolutionarily conserved are key questions. Here, we report proteome-wide steady-state protein turnover rate measurements for the evolutionarily distant but ecologically similar yeasts, Saccharomyces cerevisiae and Schizosaccharomyces pombe. We find that the half-life of most proteins is much longer than currently thought and determined to a large degree by protein synthesis and dilution due to cell division. However, we detect a significant subset of proteins (∼15%) in both yeasts that are turned over rapidly. In addition, the relative abundances of orthologous proteins between the two yeasts are highly conserved across the 400 million years of evolution. In contrast, their respective turnover rates differ considerably. Our data provide a high-confidence resource for studying protein degradation in common yeast model systems.
authors.affiliation: Department of Cell Biology, Yale School of Medicine, New Haven, CT 06510, USA; Department of Genetics and Complex Diseases, Harvard School of Public Health, Boston, MA 02115, USA.
authors.forename: Romain
authors.initials: R
authors.lastname: Christiano
authors.affiliation: Mass spectrometry core facility, Max Planck Institute of Biochemistry, 82152 Martinsried, Germany.
authors.forename: Nagarjuna
authors.initials: N
authors.lastname: Nagaraj
authors.affiliation: Department of Cell Biology, Yale School of Medicine, New Haven, CT 06510, USA; Department of Genetics and Complex Diseases, Harvard School of Public Health, Boston, MA 02115, USA.
authors.forename: Florian
authors.initials: F
authors.lastname: Fröhlich
authors.affiliation: Department of Cell Biology, Yale School of Medicine, New Haven, CT 06510, USA; Department of Genetics and Complex Diseases, Harvard School of Public Health, Boston, MA 02115, USA; Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA; Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA. Electronic address: twalther@hsph.harvard.edu.
authors.forename: Tobias C
authors.initials: TC
authors.lastname: Walther
mesh_terms: D060746:Endoplasmic Reticulum-Associated Degradation; D006207:Half-Life; D059748:Proteolysis; D020543:Proteome / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism*; D012568:Schizosaccharomyces / Q000378:metabolism; D029702:Schizosaccharomyces pombe Proteins / Q000378:metabolism*
chemical_list: D020543:Proteome; D029701:Saccharomyces cerevisiae Proteins; D029702:Schizosaccharomyces pombe Proteins