title: Cryoprotective effect of phosphorous-containing phenolic anti-oxidant for the cryopreservation of beluga sperm.
abstract: A cryoprotective effect of an addition of a new synthetic antioxidant - a representative of phosphorus-containing sterically hindered phenols is presented. The efficiency of the compound was shown to exceed the effect of lipid-soluble antioxidants butylated hydroxytoluene (BHT) and trolox in the conditions of cryopreservation of beluga sperm in the presence of the modified Stein's medium. It was shown that the level of carbonyl oxidation by-products, which can react with thiobarbituric acid (TBARS), in beluga sperm was inversely proportional to the motility time of sperm cells. The fertility of beluga sperm increased 2 times upon the addition of phosphorus-containing phenol to a modified Stein's medium. The prospects of the new antioxidant application to improve cryoresistance of beluga sperm in the conditions of cryopreservation for its efficient protection from the peroxidation processes are discussed.
authors.affiliation: Southern Scientific Center, Russian Academy of Sciences, Rostov-on-Don, Russia. Electronic address: vposipova@rambler.ru.
authors.forename: V P
authors.initials: VP
authors.lastname: Osipova
authors.affiliation: Southern Scientific Center, Russian Academy of Sciences, Rostov-on-Don, Russia.
authors.forename: M N
authors.initials: MN
authors.lastname: Kolyada
authors.affiliation: Astrakhan State Technical University, ul. Tatishcheva 16, Astrakhan 414025, Russia. Electronic address: berberova@astu.org.
authors.forename: N T
authors.initials: NT
authors.lastname: Berberova
authors.affiliation: Moscow State University, Vorob'evy gory, Moscow 119992, Russia.
authors.forename: E R
authors.initials: ER
authors.lastname: Milaeva
authors.affiliation: Southern Scientific Center, Russian Academy of Sciences, Rostov-on-Don, Russia.
authors.forename: E N
authors.initials: EN
authors.lastname: Ponomareva
authors.affiliation: Southern Scientific Center, Russian Academy of Sciences, Rostov-on-Don, Russia.
authors.forename: M M
authors.initials: MM
authors.lastname: Belaya
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000378:metabolism*; D050643:Beluga Whale; D002084:Butylated Hydroxytoluene / Q000378:metabolism; D002839:Chromans / Q000378:metabolism; D015925:Cryopreservation / Q000379:methods / Q000662:veterinary*; D003451:Cryoprotective Agents / Q000737:chemistry / Q000378:metabolism*; D005260:Female; D005306:Fertilization; D005399:Fishes / Q000502:physiology*; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D010636:Phenols / Q000737:chemistry / Q000378:metabolism*; D010758:Phosphorus / Q000737:chemistry / Q000378:metabolism; D012662:Semen Preservation / Q000379:methods / Q000662:veterinary*; D013081:Sperm Motility / Q000187:drug effects; D013094:Spermatozoa / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism
chemical_list: D000975:Antioxidants; D002839:Chromans; D003451:Cryoprotective Agents; D010636:Phenols; D002084:Butylated Hydroxytoluene; D010758:Phosphorus; C010643:6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid
keywords: Antioxidants; Beluga sperm; Cryopreservation; Lipid peroxidation; Sterically hindered phenols