title: Characterization of surface phenotypic molecules of teleost dendritic cells.
abstract: Dendritic cells (DCs) are among the most important professional antigen-presenting cells (APCs) that participate in various biological activities in mammals. However, evidence of the existence of DCs in teleost fish and other lower vertebrates remains limited. In this study, phenotypic and functional characteristics of teleost DCs were described in a zebrafish model. An improved method to efficiently enrich DCs was established. Immunofluorescence staining revealed that the surface phenotypic hallmarks of mammalian DCs, including MHC-II, CD80/86, CD83, and CD209, were distributed on the surfaces of zebrafish DCs (DrDCs). Functional analysis results showed that DrDCs could initiate antigen-specific CD4(+) T cell activation, in which MHC-II, CD80/86, CD83, and CD209 are implicated. Hence, teleost DCs exhibit conserved immunophenotypes and functions similar to those of their mammalian counterparts. Our findings contributed to the current understanding of the evolutionary history of DCs and the DC-regulatory mechanisms of adaptive immunity.
authors.affiliation: College of Life Sciences, Zhejiang University, Hangzhou 310058, China; Key Laboratory for Cell and Gene Engineering of Zhejiang Province, Hangzhou 310058, China.
authors.forename: Tong
authors.initials: T
authors.lastname: Shao
authors.affiliation: College of Science, National University of Defense Technology, Changsha, Hunan 410073, China.
authors.forename: Lv-Yun
authors.initials: LY
authors.lastname: Zhu
authors.affiliation: College of Life Sciences, Zhejiang University, Hangzhou 310058, China; Key Laboratory for Cell and Gene Engineering of Zhejiang Province, Hangzhou 310058, China.
authors.forename: Li
authors.initials: L
authors.lastname: Nie
authors.affiliation: College of Life Sciences, Zhejiang University, Hangzhou 310058, China; Key Laboratory for Cell and Gene Engineering of Zhejiang Province, Hangzhou 310058, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Shi
authors.affiliation: College of Life Sciences, Zhejiang University, Hangzhou 310058, China; Key Laboratory for Cell and Gene Engineering of Zhejiang Province, Hangzhou 310058, China.
authors.forename: Wei-Ren
authors.initials: WR
authors.lastname: Dong
authors.affiliation: College of Life Sciences, Zhejiang University, Hangzhou 310058, China; Key Laboratory for Cell and Gene Engineering of Zhejiang Province, Hangzhou 310058, China. Electronic address: xianglx@zju.edu.cn.
authors.forename: Li-Xin
authors.initials: LX
authors.lastname: Xiang
authors.affiliation: College of Life Sciences, Zhejiang University, Hangzhou 310058, China; Key Laboratory for Cell and Gene Engineering of Zhejiang Province, Hangzhou 310058, China. Electronic address: shaojz@zju.edu.cn.
authors.forename: Jian-Zhong
authors.initials: JZ
authors.lastname: Shao
mesh_terms: D000818:Animals; D000938:Antigen-Presenting Cells / Q000276:immunology* / Q000378:metabolism; D015703:Antigens, CD / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D018122:B7-1 Antigen / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D015815:Cell Adhesion Molecules / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D002462:Cell Membrane / Q000276:immunology* / Q000378:metabolism; D003713:Dendritic Cells / Q000276:immunology* / Q000378:metabolism; D005260:Female; D005434:Flow Cytometry; D050989:GATA2 Transcription Factor / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D015870:Gene Expression / Q000276:immunology; D000949:Histocompatibility Antigens Class II / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D007136:Immunoglobulins / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D016130:Immunophenotyping; D053762:Interleukin-12 Subunit p40 / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D037181:Lectins, C-Type / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D008213:Lymphocyte Activation / Q000276:immunology; D008297:Male; D008562:Membrane Glycoproteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D018613:Microscopy, Confocal; D016186:Receptor, Macrophage Colony-Stimulating Factor / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D011948:Receptors, Antigen, T-Cell / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015027:Zebrafish / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D029961:Zebrafish Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D000097802:CD83 Antigen; D000099215:DC-Specific ICAM-3 Grabbing Nonintegrin
chemical_list: D015703:Antigens, CD; D018122:B7-1 Antigen; D015815:Cell Adhesion Molecules; D050989:GATA2 Transcription Factor; D000949:Histocompatibility Antigens Class II; D007136:Immunoglobulins; D053762:Interleukin-12 Subunit p40; D037181:Lectins, C-Type; D008562:Membrane Glycoproteins; D016186:Receptor, Macrophage Colony-Stimulating Factor; D011948:Receptors, Antigen, T-Cell; D011956:Receptors, Cell Surface; D029961:Zebrafish Proteins; D000097802:CD83 Antigen; D000099215:DC-Specific ICAM-3 Grabbing Nonintegrin; C516059:Gata2a protein, zebrafish
keywords: Antigen presentation; Co-stimulatory signals; Surface phenotypic molecules; Zebrafish dendritic cells