title: Effect of trace hydrazine addition on the functional bacterial community of a sequencing batch reactor performing completely autotrophic nitrogen removal over nitrite.
abstract: A sequencing batch reactor (SBR) was conducted to perform completely autotrophic nitrogen removal over nitrite (CANON). The effect of long-term trace N2H4 addition on ammonium oxidizing bacteria (AOB) and anaerobic AOB (AnAOB) in the CANON system was investigated. AOB and AnAOB primarily related to Nitrosococcus, Nitrosomonas and Candidatus scalindua, respectively. Before and after trace N2H4 addition, the estimates of AOB population decreased from 1.03×10(7) to 6.25×10(4)copies/g (dry sludge), but that of AnAOB increased from 3.14×10(9) to 5.86×10(10)copies/g (dry sludge). Despite there was a partially negative impact on AOB growth, the trace N2H4 addition exerted a stronger inhibition on nitrite oxidizing bacteria (NOB) and promoted AnAOB growth, which improved the nitrogen removal of the CANON system. Sludge granules enriched under long-term trace N2H4 addition were spherical and ellipsoidal, and the aerobic AOB were mainly located on the outer layers while AnAOB occupied most of the interior parts.
authors.affiliation: School of Chemical Engineering, Chongqing University of Technology, Chongqing 400054, People's Republic of China; Department of Environmental Science, Chongqing University, Chongqing 400044, People's Republic of China.
authors.forename: Pengying
authors.initials: P
authors.lastname: Xiao
authors.affiliation: State Key Laboratory of Coal Mine Disaster Dynamics and Control, Chongqing University, Chongqing 400044, People's Republic of China; Department of Environmental Science, Chongqing University, Chongqing 400044, People's Republic of China. Electronic address: lupl@cqu.edu.cn.
authors.forename: Peili
authors.initials: P
authors.lastname: Lu
authors.affiliation: State Key Laboratory of Coal Mine Disaster Dynamics and Control, Chongqing University, Chongqing 400044, People's Republic of China; Department of Environmental Science, Chongqing University, Chongqing 400044, People's Republic of China.
authors.forename: Daijun
authors.initials: D
authors.lastname: Zhang
authors.affiliation: College of Life Sciences, Henan Normal University, Henan 453007, People's Republic of China.
authors.forename: Xinkuan
authors.initials: X
authors.lastname: Han
authors.affiliation: College of Life Sciences, Henan Normal University, Henan 453007, People's Republic of China.
authors.forename: Qingxiang
authors.initials: Q
authors.lastname: Yang
mesh_terms: D064751:Ammonium Compounds / Q000378:metabolism; D052818:Autotrophic Processes; D001419:Bacteria / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D019149:Bioreactors / Q000382:microbiology*; D006834:Hydrazines / Q000378:metabolism*; D059013:Microbial Consortia / Q000235:genetics / Q000502:physiology; D008855:Microscopy, Electron, Scanning; D009573:Nitrites / Q000378:metabolism*; D009584:Nitrogen / Q000378:metabolism*; D009606:Nitrosomonas / Q000378:metabolism; D016133:Polymerase Chain Reaction; D012722:Sewage / Q000382:microbiology
chemical_list: D064751:Ammonium Compounds; D006834:Hydrazines; D009573:Nitrites; D012722:Sewage; C029424:hydrazine; D009584:Nitrogen
keywords: Aerobic ammonium oxidizing bacteria; Anaerobic ammonium oxidizing; Completely autotrophic nitrogen removal over nitrite; N(2)H(4)