title: Multibiomarker assessment of cerium dioxide nanoparticle (nCeO2) sublethal effects on two freshwater invertebrates, Dreissena polymorpha and Gammarus roeseli.
abstract: Cerium nanoparticles (nCeO2) are widely used in everyday products, as fuel and paint additives. Meanwhile, very few studies on nCeO2 sublethal effects on aquatic organisms are available. We tried to fill this knowledge gap by investigating short-term effects of nCeO2 at environmentally realistic concentrations on two freshwater invertebrates; the amphipod Gammarus roeseli and the bivalve Dreissena polymorpha, using an integrated multibiomarker approach to detect early adverse effects of nCeO2 on organism biology. Differences in the behaviour of the organisms and of nanoparticles in the water column led to differential nCeO2 bioaccumulations, G. roeseli accumulating more cerium than D. polymorpha. Exposure to nCeO2 led to decreases in the size of the lysosomal system, catalase activity and lipoperoxidation in mussel digestive glands that could result from nCeO2 antioxidant properties, but also negatively impacted haemolymph ion concentrations. At the same time, no strong adverse effects of nCeO2 could be observed on G. roeseli. Further experiments will be necessary to confirm the absence of severe nCeO2 adverse effects in long-term environmentally realistic conditions.
authors.affiliation: Université de Lorraine, CNRS UMR 7360, Laboratoire Interdisciplinaire des Environnements Continentaux (LIEC), Campus Bridoux, Rue du Général Delestraint, 57070 Metz, France; International Consortium for the Environmental Implications of Nanotechnology (iCEINT), Aix en Provence, France.
authors.forename: M
authors.initials: M
authors.lastname: Garaud
authors.affiliation: Université de Lorraine, CNRS UMR 7360, Laboratoire Interdisciplinaire des Environnements Continentaux (LIEC), Campus Bridoux, Rue du Général Delestraint, 57070 Metz, France.
authors.forename: J
authors.initials: J
authors.lastname: Trapp
authors.affiliation: Université de Lorraine, CNRS UMR 7360, Laboratoire Interdisciplinaire des Environnements Continentaux (LIEC), Campus Bridoux, Rue du Général Delestraint, 57070 Metz, France.
authors.forename: S
authors.initials: S
authors.lastname: Devin
authors.affiliation: Université de Lorraine, CNRS UMR 7360, Laboratoire Interdisciplinaire des Environnements Continentaux (LIEC), Campus Bridoux, Rue du Général Delestraint, 57070 Metz, France.
authors.forename: C
authors.initials: C
authors.lastname: Cossu-Leguille
authors.affiliation: Université de Lorraine, CNRS UMR 7360, Laboratoire Interdisciplinaire des Environnements Continentaux (LIEC), Campus Bridoux, Rue du Général Delestraint, 57070 Metz, France.
authors.forename: S
authors.initials: S
authors.lastname: Pain-Devin
authors.affiliation: Université de Lorraine, CNRS UMR 7360, Laboratoire Interdisciplinaire des Environnements Continentaux (LIEC), Campus Bridoux, Rue du Général Delestraint, 57070 Metz, France.
authors.forename: V
authors.initials: V
authors.lastname: Felten
authors.affiliation: Université de Lorraine, CNRS UMR 7360, Laboratoire Interdisciplinaire des Environnements Continentaux (LIEC), Campus Bridoux, Rue du Général Delestraint, 57070 Metz, France; International Consortium for the Environmental Implications of Nanotechnology (iCEINT), Aix en Provence, France. Electronic address: laure.giamberini@univ-lorraine.fr.
authors.forename: L
authors.initials: L
authors.lastname: Giamberini
mesh_terms: D033304:Amphipoda / Q000187:drug effects* / Q000378:metabolism; D000818:Animals; D015415:Biomarkers / Q000032:analysis; D002374:Catalase / Q000378:metabolism; D002563:Cerium / Q000378:metabolism / Q000633:toxicity*; D049875:Dreissena / Q000187:drug effects* / Q000378:metabolism; D004789:Enzyme Activation / Q000187:drug effects; D005618:Fresh Water; D053758:Nanoparticles / Q000378:metabolism / Q000633:toxicity*; D010084:Oxidation-Reduction / Q000187:drug effects; D014874:Water Pollutants, Chemical / Q000378:metabolism / Q000633:toxicity*
chemical_list: D015415:Biomarkers; D014874:Water Pollutants, Chemical; D002563:Cerium; C030583:ceric oxide; D002374:Catalase
keywords: Biomarker; Cerium; Dreissena polymorpha; Ecotoxicology; Gammarus roeseli; Nanoparticle