title: Direct comparison of different coating matrix on the hepatic differentiation from adipose-derived stem cells.
abstract: Various extracellular matrix components were employed as coating materials to promote hepatic differentiation from ADSCs. However, no consensus was achieved about the optimal coating matrix due to the lack of direct comparison among different coating matrix. In this study, several coating extracellular matrixs were used for hepatic differentiation of ADSCs and direct comparison between them was performed. We demonstrated that liver DCM as coating matrix could significantly enhance the hepatic differentiation from ADSCs compared with collagen, fibronectin and Matrigel both in the presence and absence of GFs, including enhanced hepatocyte-specific genes expression, hepatocyte related protein secretion with improved liver functions. And the differentiated cells also exhibited the characteristics of mature hepatocytes. In conclusion, the study proved an effective hepatic-inducing method and indicated that DCM could promote the differentiation of ADSCs into hepatocyte-like cells, which demonstrates feasibility of liver DCM as a bio-scaffold for liver regenerative medicine and tissue engineering.
authors.affiliation: Hospital and Institute of Hepatobiliary Surgery, Chinese PLA General Hospital, 28th Fu Xing Road, Beijing 100853, People's Republic of China.
authors.forename: Xuan
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Hospital and Institute of Hepatobiliary Surgery, Chinese PLA General Hospital, 28th Fu Xing Road, Beijing 100853, People's Republic of China. Electronic address: dongjh301@163.com.
authors.forename: Jiahong
authors.initials: J
authors.lastname: Dong
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D002469:Cell Separation; D004247:DNA / Q000378:metabolism; D005109:Extracellular Matrix / Q000378:metabolism*; D005782:Gels / Q000494:pharmacology; D008099:Liver / Q000166:cytology* / Q000187:drug effects; D008810:Mice, Inbred C57BL; D017207:Rats, Sprague-Dawley; D013234:Stem Cells / Q000166:cytology* / Q000187:drug effects
chemical_list: D005782:Gels; D004247:DNA
keywords: Adipose-derived mesenchymal stem cells (ADSCs); Decellularized extracellular matrix (DCM); Differentiation; Liver