title: Influence of adult Anguillicoloides crassus load in European eels swimbladder on macrophage response.
abstract: Anguillicoloides crassus has become one of the most important threats to the European eel (Anguilla anguilla). Adult parasites colonize the swimbladder leading to an impaired functioning of this organ. The infection is also responsible for an increased in the stress level of infected eels, that could produce an altered immune response as well. Differences in parasite loads and effects in the European and Japanese eel (Anguilla japonica) have been described. We have studied the influence of the number of adult parasites present in the swimbladder of wild eels on the macrophage response (phagocytosis and respiratory burst) as part of the first immune response to pathogens. Our results show an increased phagocytozed bacterial survival 24 h post-infection in macrophages of eels infected with more than ten adult parasites compared to macrophages from eels infected with less than those ten adult parasites. Respiratory burst results also showed a less efficient response in macrophages from eels infected with more than ten adult parasites, although in this case results were not found to be significant.
authors.affiliation: Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad de Murcia, E-30100 Murcia, Spain; Campus de Excelencia Internacional Regional "Campus Mare Nostrum", Spain.
authors.forename: P
authors.initials: P
authors.lastname: Muñoz
authors.affiliation: Campus de Excelencia Internacional Regional "Campus Mare Nostrum", Spain; Servicio de Pesca y Acuicultura, D.G. de Ganadería y Pesca, Consejería de Agricultura y Agua de la Región de Murcia, Juan XXIII, 30071 Murcia, Spain.
authors.forename: J
authors.initials: J
authors.lastname: Peñalver
authors.affiliation: Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad de Murcia, E-30100 Murcia, Spain; Campus de Excelencia Internacional Regional "Campus Mare Nostrum", Spain.
authors.forename: R
authors.initials: R
authors.lastname: Ruiz de Ybañez
authors.affiliation: Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad Complutense de Madrid, Avda. Puerta de Hierro s/n, 28040 Madrid, Spain. Electronic address: gcabrera@ucm.es.
authors.forename: J
authors.initials: J
authors.lastname: Garcia
mesh_terms: D000400:Air Sacs / Q000469:parasitology; D000811:Anguilla; D000818:Animals; D017176:Dracunculoidea / Q000502:physiology*; D005393:Fish Diseases / Q000276:immunology* / Q000469:parasitology; D008264:Macrophages / Q000276:immunology* / Q000469:parasitology; D010587:Phagocytosis; D016897:Respiratory Burst; D013030:Spain; D017205:Spirurida Infections / Q000276:immunology / Q000469:parasitology / Q000662:veterinary*; D015009:Yersinia Infections / Q000276:immunology / Q000469:parasitology / Q000662:veterinary*; D044124:Yersinia ruckeri / Q000502:physiology
keywords: Anguillicoloides crassus; European eel; Macrophages; Phagocytosis; Respiratory burst