title: A recombinant truncated surface immunogenic protein (tSip) plus adjuvant FIA confers active protection against Group B streptococcus infection in tilapia.
abstract: OBJECTIVE
  Tilapia is an important agricultural fish that has been plagued by Group B streptococcus (GBS) infections in recent years, some of them severe. It is well-known that surface immunogenicity protein (Sip) is an effective vaccine against GBS.
  
  
  METHODS
  Since Sip was not expressed in either E. coli BL21 or E. coli Rosetta, we removed the N-terminal signal peptide and LysM of the virus to produce purified truncated Sip (tSip(1)), which multiplied easily in an E. coli host. The antibody's ability to recognize and combine with GBS was determined by Western-blot and specific staining in vitro. The relative percentage of survival (RPS), antibody titers, bacterial recovery, and pathologic morphology were monitored in vivo to evaluate the immune effects. Freund's incomplete adjuvant (FIA) plus tSip and aluminum hydroxide gel (AH) plus tSip were also evaluated.
  
  
  RESULTS
  It revealed that tSip mixed with FIA was an effective vaccine against GBS in tilapia, while AH is toxic to tilapia.
authors.affiliation: Department of Basic Veterinary, Veterinary Medicine College, Sichuan Agricultural University, Ya'an 625014, Sichuan, PR China.
authors.forename: Yang
authors.initials: Y
authors.lastname: He
authors.affiliation: Department of Basic Veterinary, Veterinary Medicine College, Sichuan Agricultural University, Ya'an 625014, Sichuan, PR China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Ya'an 625014, Sichuan, PR China. Electronic address: kywang@sicau.edu.cn.
authors.forename: Kai-Yu
authors.initials: KY
authors.lastname: Wang
authors.affiliation: Animal Health Research Institute of Tongwei Co., Ltd., Chengdu 610041, Sichuan, PR China.
authors.forename: Dan
authors.initials: D
authors.lastname: Xiao
authors.affiliation: Department of Aquaculture, College of Animal Science& Technology, Sichuan Agricultural University, Ya'an, Sichuan 625014, PR China.
authors.forename: De-Fang
authors.initials: DF
authors.lastname: Chen
authors.affiliation: Department of Basic Veterinary, Veterinary Medicine College, Sichuan Agricultural University, Ya'an 625014, Sichuan, PR China.
authors.forename: Lingyuan
authors.initials: L
authors.lastname: Huang
authors.affiliation: Animal Health Research Institute of Tongwei Co., Ltd., Chengdu 610041, Sichuan, PR China.
authors.forename: Tianqiang
authors.initials: T
authors.lastname: Liu
authors.affiliation: Department of Basic Veterinary, Veterinary Medicine College, Sichuan Agricultural University, Ya'an 625014, Sichuan, PR China.
authors.forename: Jun
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Basic Veterinary, Veterinary Medicine College, Sichuan Agricultural University, Ya'an 625014, Sichuan, PR China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Ya'an 625014, Sichuan, PR China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Geng
authors.affiliation: Department of Basic Veterinary, Veterinary Medicine College, Sichuan Agricultural University, Ya'an 625014, Sichuan, PR China.
authors.forename: Er-Long
authors.initials: EL
authors.lastname: Wang
authors.affiliation: Department of Basic Veterinary, Veterinary Medicine College, Sichuan Agricultural University, Ya'an 625014, Sichuan, PR China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Yang
mesh_terms: D000276:Adjuvants, Immunologic / Q000008:administration & dosage*; D000536:Aluminum Hydroxide / Q000008:administration & dosage; D000818:Animals; D000942:Antigens, Bacterial / Q000235:genetics / Q000276:immunology*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005393:Fish Diseases / Q000382:microbiology / Q000517:prevention & control*; D005620:Freund's Adjuvant / Q000008:administration & dosage*; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology; D013290:Streptococcal Infections / Q000517:prevention & control / Q000662:veterinary*; D022541:Streptococcal Vaccines / Q000008:administration & dosage* / Q000235:genetics / Q000302:isolation & purification; D013292:Streptococcus agalactiae / Q000235:genetics / Q000276:immunology*; D016019:Survival Analysis; D017210:Tilapia; D016896:Treatment Outcome; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000235:genetics / Q000302:isolation & purification
chemical_list: D000276:Adjuvants, Immunologic; D000942:Antigens, Bacterial; D011994:Recombinant Proteins; C414463:SIP protein, Streptococcus group B; D022541:Streptococcal Vaccines; D014614:Vaccines, Synthetic; D000536:Aluminum Hydroxide; D005620:Freund's Adjuvant
keywords: Adjuvant; Group B streptococcus; Tilapia; Truncated Sip; Vaccine