title: Impact of microsatellite instability on survival of endometrial cancer patients.
abstract: OBJECTIVE
  Endometrial cancer (EC) is the most commonly diagnosed gynecologic malignancy among women worldwide and may be classified on the basis of different molecular, pathologic and genetic alterations, including microsatellite instability (MSI). Although MSI is associated with a more favorable outcome in colorectal cancer, its relationship with prognosis in EC cancer is not yet clear. The aim of our study is to identify whether MSI correlates with survival of patients in EC.
  
  
  METHODS
  We examined MSI status and survival of 109 women. MSI was detected by employing the Promega MSI Analysis System, which used 5 mononucleotides markers (BAT-25, BAT-26, NR-21, NR-24, and MONO-27) to identify MSI in a tumor and normal tissue DNA and 2 pentanucleotide markers (Penta C and Penta D) for specimen identification. Median follow-up of patients was 40.4 months (range 5.2-47.9). Survival was estimated by the Kaplan-Meier method and Cox regression analysis was used to assess the effects of different variables on patient survival.
  
  
  RESULTS
  MSI-high was detected in 15.6% EC cases, all of which were associated with endometrioid type histology. Kaplan-Meier survival analysis showed no statistically significant differences between patients with MSI-high and MSI stable tumors (P=0.4) and multivariate analysis concluded that MSI status remained insignificant after stage, histology and tumor grade adjustment (P=0.5).
  
  
  CONCLUSIONS
  Our study showed no statistically significant relationship between MSI-high and survival of endometrial cancer patients.
authors.affiliation: National Cancer Institute, Vilnius, Lithuania. Electronic address: daiva.kanopiene@vuoi.lt.
authors.forename: Daiva
authors.initials: D
authors.lastname: Kanopienė
authors.affiliation: National Cancer Institute, Vilnius, Lithuania.
authors.forename: Giedrė
authors.initials: G
authors.lastname: Smailytė
authors.affiliation: Promega Corporation, Madison, WI, USA.
authors.forename: Jolanta
authors.initials: J
authors.lastname: Vidugirienė
authors.affiliation: Promega Corporation, Madison, WI, USA.
authors.forename: Jeff
authors.initials: J
authors.lastname: Bacher
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D016889:Endometrial Neoplasms / Q000235:genetics* / Q000401:mortality*; D005260:Female; D006801:Humans; D053208:Kaplan-Meier Estimate; D053842:Microsatellite Instability; D008875:Middle Aged; D009154:Mutation; D011379:Prognosis
keywords: Endometrial cancer; Microsatellite instability; Survival