title: Role of BC040587 as a predictor of poor outcome in breast cancer.
abstract: BACKGROUND
  Accumulating studies have focused on the oncogenic and tumor suppressive roles of the newly identified lncRNAs. A novel lncRNA BC040587 in 3q13.31 locus which exists frequent copy number alterations was found to be associated with poor survival of osteosarcoma patients. However, its role in breast cancer (BC) remains unknown. The aim of this study was to examine the expression pattern of BC040587 in BC and to evaluate its biological role and clinical significance in prediction of prognosis.
  
  
  METHODS
  Expression of BC040587 was analyzed in 20 pairs of BC cancer tissues and adjacent noncancerous tissues (ANCT), also in 151 BC tissues, 9 BC cell lines and one normal breast cell line by quantitative reverse-transcription polymerase chain reaction (qRT-PCR). Differences between groups were tested for significance using Student's t-test (two-tailed). Then we analyzed the potential relationship between BC040587 expression and clinic pathological features of BC patients. The correlation was analyzed by SPSS software.
  
  
  RESULTS
  It showed that BC040587 expression was down regulated both in BC samples and in BC cell lines compared with corresponding normal control. BC040587 expression was correlated with menopausal status (p = 0.040) and tumor differentiation (p = 0.035). The Kaplan-Meier survival curves indicated that the overall survival (OS) was significantly poor in low BC040587 expression BC patients (p = 0.023). Furthermore, expression of BC040587 was significantly associated with worse prognosis and was shown to be an independent prognostic marker breast cancer (p = 0.032). Our studies indicate that BC040587 may represent a new marker of prognosis in breast cancer.
  
  
  CONCLUSIONS
  Our studies indicate that BC040587 is significantly down-regulated in BC tissues and BC cell lines. BC040587 may represent a new marker of prognosis in breast cancer.
authors.affiliation: Breast Cancer Institute, Fudan University Shanghai Cancer Center, Shanghai, 200032 China ; Department of Oncology, Shanghai Medical College, Fudan University, Shanghai, 200032 China.
authors.forename: Yayun
authors.initials: Y
authors.lastname: Chi
authors.affiliation: Breast Cancer Institute, Fudan University Shanghai Cancer Center, Shanghai, 200032 China ; Department of Breast Surgery, Fudan University Shanghai Cancer Center, Shanghai, 200032 China ; Department of Oncology, Shanghai Medical College, Fudan University, Shanghai, 200032 China.
authors.forename: Sheng
authors.initials: S
authors.lastname: Huang
authors.affiliation: Department of Pathology, Fudan University Shanghai Cancer Center, Shanghai, 200032 China ; Department of Oncology, Shanghai Medical College, Fudan University, Shanghai, 200032 China.
authors.forename: Lin
authors.initials: L
authors.lastname: Yuan
authors.affiliation: Breast Cancer Institute, Fudan University Shanghai Cancer Center, Shanghai, 200032 China ; Department of Breast Surgery, Fudan University Shanghai Cancer Center, Shanghai, 200032 China ; Department of Oncology, Shanghai Medical College, Fudan University, Shanghai, 200032 China.
authors.forename: Mengying
authors.initials: M
authors.lastname: Liu
authors.affiliation: Breast Cancer Institute, Fudan University Shanghai Cancer Center, Shanghai, 200032 China ; Department of Breast Surgery, Fudan University Shanghai Cancer Center, Shanghai, 200032 China ; Department of Oncology, Shanghai Medical College, Fudan University, Shanghai, 200032 China.
authors.forename: Naisi
authors.initials: N
authors.lastname: Huang
authors.affiliation: Department of Pathology, Fudan University Shanghai Cancer Center, Shanghai, 200032 China ; Department of Oncology, Shanghai Medical College, Fudan University, Shanghai, 200032 China.
authors.forename: Shuling
authors.initials: S
authors.lastname: Zhou
authors.affiliation: Department of Breast Surgery, Fudan University Shanghai Cancer Center, Shanghai, 200032 China.
authors.forename: Bingqing
authors.initials: B
authors.lastname: Zhou
authors.affiliation: Breast Cancer Institute, Fudan University Shanghai Cancer Center, Shanghai, 200032 China ; Department of Breast Surgery, Fudan University Shanghai Cancer Center, Shanghai, 200032 China ; Department of Oncology, Shanghai Medical College, Fudan University, Shanghai, 200032 China.
authors.forename: Jiong
authors.initials: J
authors.lastname: Wu
keywords: BC040587; Breast cancer; Long non-coding RNA; Prognosis; Tumor suppressor