title: Isolation and characterization of an antifungal protein from Bacillus licheniformis HS10.
abstract: Bacillus licheniformis HS10 is a good biocontrol agent against Pseudoperonospora cubensis which caused cucumber downy disease. To identify and characterize the antifungal proteins produced by B.licheniformis HS10, the proteins from HS10 were isolated by using 30-60% ammonium sulfate precipitation, and purified with column chromatography on DEAE Sepharose Fast Flow, RESOURCE Q and Sephadex G-75. And the SDS-PAGE and MALDI-TOF/TOF-MS analysis results demonstrated that the antifungal protein was a monomer with molecular weight of about 55 kDa, identified as carboxypeptidase. Our experiments also showed that the antifungal protein from B. licheniformis HS10 had significantly inhibition on eight different kinds of plant pathogenic fungi, and it was stable with good biological activity at as high as 100°C for 30 min and in pH value ranged from 6 to 10. The biological activity was negatively affected by protease K and 10mM metal cations except Ca(2+).
authors.affiliation: Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China; Engineering Center of Bioresource Pesticide in Jiangsu Province, Nanjing 210095, China; Key Laboratory of Integrated Management of Crop Diseases and Pests (Nanjing Agricultural University), Ministry of Education, Nanjing 210095, China.
authors.forename: Zhixin
authors.initials: Z
authors.lastname: Wang
authors.affiliation: College of Life Science and Chemical Engineering, Huaiyin Institute of Technology, Huai'an 223003, China.
authors.forename: Yunpeng
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China; Engineering Center of Bioresource Pesticide in Jiangsu Province, Nanjing 210095, China; Key Laboratory of Integrated Management of Crop Diseases and Pests (Nanjing Agricultural University), Ministry of Education, Nanjing 210095, China; Chinese Academy of Tropical Agricultural Sciences Guangzhou Experimental Station, Guangzhou 510140, China; Tropical Energy and Ecology Research Centre of CATAS, Guangzhou 510140, China.
authors.forename: Li
authors.initials: L
authors.lastname: Zheng
authors.affiliation: Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China; Engineering Center of Bioresource Pesticide in Jiangsu Province, Nanjing 210095, China; Key Laboratory of Integrated Management of Crop Diseases and Pests (Nanjing Agricultural University), Ministry of Education, Nanjing 210095, China.
authors.forename: Xiaona
authors.initials: X
authors.lastname: Yang
authors.affiliation: Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China; Engineering Center of Bioresource Pesticide in Jiangsu Province, Nanjing 210095, China; Key Laboratory of Integrated Management of Crop Diseases and Pests (Nanjing Agricultural University), Ministry of Education, Nanjing 210095, China. Electronic address: hxliu@njau.edu.cn.
authors.forename: Hongxia
authors.initials: H
authors.lastname: Liu
authors.affiliation: Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China; Engineering Center of Bioresource Pesticide in Jiangsu Province, Nanjing 210095, China; Key Laboratory of Integrated Management of Crop Diseases and Pests (Nanjing Agricultural University), Ministry of Education, Nanjing 210095, China.
authors.forename: Jianhua
authors.initials: J
authors.lastname: Guo
mesh_terms: D000935:Antifungal Agents / Q000302:isolation & purification* / Q000494:pharmacology; D023181:Antimicrobial Cationic Peptides / Q000302:isolation & purification / Q000494:pharmacology; D001407:Bacillus / Q000737:chemistry / Q000502:physiology*; D001426:Bacterial Proteins / Q000302:isolation & purification* / Q000494:pharmacology; D061046:Biological Control Agents; D002268:Carboxypeptidases / Q000302:isolation & purification / Q000494:pharmacology; D018553:Cucumis sativus / Q000382:microbiology; D005658:Fungi / Q000187:drug effects / Q000472:pathogenicity; D009181:Mycoses / Q000382:microbiology / Q000517:prevention & control; D009868:Oomycetes / Q000187:drug effects / Q000472:pathogenicity; D010935:Plant Diseases / Q000382:microbiology / Q000517:prevention & control
chemical_list: D000935:Antifungal Agents; D023181:Antimicrobial Cationic Peptides; D001426:Bacterial Proteins; D061046:Biological Control Agents; D002268:Carboxypeptidases
keywords: Antifungal protein; Bacillus licheniformis; Biocontrol; Chromatography