title: New drug-eluting lenses to be applied as bandages after keratoprosthesis implantation.
abstract: Corneal tissue is the most commonly transplanted tissue worldwide. This work aimed to develop a new drug-eluting contact lens that may be used as a bandage after keratoprosthesis. During this work, films were produced using poly(vinyl alcohol) (PVA) and chitosan (CS) crosslinked with glyoxal (GL). Vancomycin chlorhydrate (VA) was impregnated in these systems by soaking. Attenuated total reflectance - Fourier transform infrared spectroscopy was used to confirm crosslinking. The cytotoxic and drug release profile, hydrophilicity, thermal and biodegradation as well as swelling capacity of the samples were assessed through in vitro studies. PVA and PVA/CS films were obtained by crosslinking with GL. The films were transparent, flexible with smooth surfaces, hydrophilic and able to load and release vancomycin for more than 8h. Biodegradation in artificial lachrymal fluid (ALF) with lysozyme at 37°C showed that mass loss was higher for the samples containing CS. Also, the samples prepared with CS showed the formation of pores which were visualized by SEM. All samples revealed a biocompatible character after 24h in contact with cornea endothelial cells. As a general conclusion it was possible to determine that the 70PVA/30CS film showed to combine the necessary features to prepare vancomycin-eluting contact lenses to prevent inflammation after corneal substitution.
authors.affiliation: Chemical Process Engineering and Forest Products Research Centre, Department of Chemical Engineering, University of Coimbra, Rua Sílvio Lima, Polo II, 3030-790 Coimbra, Portugal.
authors.forename: A S
authors.initials: AS
authors.lastname: Carreira
authors.affiliation: Chemical Process Engineering and Forest Products Research Centre, Department of Chemical Engineering, University of Coimbra, Rua Sílvio Lima, Polo II, 3030-790 Coimbra, Portugal. Electronic address: pferreira@eq.uc.pt.
authors.forename: P
authors.initials: P
authors.lastname: Ferreira
authors.affiliation: CICS-UBI, Centro de Investigação em Ciências da Saúde, Faculdade de Ciências da Saúde, Universidade da Beira Interior, 6200-506 Covilhã, Portugal; UDI-IPG, Research Unit for Inland Development, Polytechnic Institute of Guarda, Guarda, Portugal.
authors.forename: M P
authors.initials: MP
authors.lastname: Ribeiro
authors.affiliation: CICS-UBI, Centro de Investigação em Ciências da Saúde, Faculdade de Ciências da Saúde, Universidade da Beira Interior, 6200-506 Covilhã, Portugal.
authors.forename: T R
authors.initials: TR
authors.lastname: Correia
authors.affiliation: CICS-UBI, Centro de Investigação em Ciências da Saúde, Faculdade de Ciências da Saúde, Universidade da Beira Interior, 6200-506 Covilhã, Portugal; UDI-IPG, Research Unit for Inland Development, Polytechnic Institute of Guarda, Guarda, Portugal.
authors.forename: P
authors.initials: P
authors.lastname: Coutinho
authors.affiliation: CICS-UBI, Centro de Investigação em Ciências da Saúde, Faculdade de Ciências da Saúde, Universidade da Beira Interior, 6200-506 Covilhã, Portugal.
authors.forename: I J
authors.initials: IJ
authors.lastname: Correia
authors.affiliation: Chemical Process Engineering and Forest Products Research Centre, Department of Chemical Engineering, University of Coimbra, Rua Sílvio Lima, Polo II, 3030-790 Coimbra, Portugal.
authors.forename: M H
authors.initials: MH
authors.lastname: Gil
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage* / Q000737:chemistry; D001458:Bandages; D001672:Biocompatible Materials / Q000737:chemistry; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D048271:Chitosan / Q000737:chemistry; D003261:Contact Lenses; D016039:Corneal Transplantation / Q000379:methods*; D003432:Cross-Linking Reagents / Q000737:chemistry; D004337:Drug Carriers / Q000737:chemistry*; D016503:Drug Delivery Systems; D065546:Drug Liberation; D042783:Endothelial Cells / Q000187:drug effects; D006037:Glyoxal / Q000737:chemistry; D015394:Molecular Structure; D011142:Polyvinyl Alcohol / Q000737:chemistry; D011817:Rabbits; D017550:Spectroscopy, Fourier Transform Infrared; D014640:Vancomycin / Q000008:administration & dosage* / Q000737:chemistry
chemical_list: D000900:Anti-Bacterial Agents; D001672:Biocompatible Materials; D003432:Cross-Linking Reagents; D004337:Drug Carriers; D006037:Glyoxal; D014640:Vancomycin; D011142:Polyvinyl Alcohol; D048271:Chitosan
keywords: Chitosan; Drug-eluting lens; Glyoxal; Keratoprosthesis; Poly(vinyl alcohol)