title: Evaluation of cardio-protective effect of soybean oligosaccharides.
abstract: The aim of the present study was to investigate whether soybean oligosaccharides (SO) protects heart function against myocardium ischemia reperfusion (MIR) injury. Hearts were 20min global ischemia and 50min reperfusion. Rats were fed for 30days with saline (sham and MIR groups) or the SO (200 or 400mg/kg body weight, daily). At the end of 30days, the left main coronary artery was occluded for 30min, followed by 24h reperfusion, in anesthetized rats. Sham operated animals were subjected to the same surgical procedures, except that the suture under the left anterior descending coronary artery was not tied. Results showed that SO decreased malondialdehyde (MDA) level and increased antioxidant enzymes activities in the SO-treatment group. Pre-treated with SO it showed a significant recovery in cardiac contractile function, reduction in infarct size, and decrease in creatine kinase (CK), aspartate transaminase (AST) and lactate dehydrogenase (LDH) activities. Moreover, SO also significantly increased the expression of p-JAK2 and p-STAT3 proteins in rat heart. However, no significant change in JAK2 and STAT3 levels was observed. Activation of JAK2/STAT3 pathway showed a significant protective role in the SO-treatment group. Perhaps, the altered activation of the JAK2/STAT3 pathway in ischemic myocardium is one mechanism by which SO is cardioprotective.
authors.affiliation: Department of Cardiology, Affiliated hospital of Qingdao University, Qingdao, 266021, China; Department of Cardiology, Affiliated Hospital of Jining Medical University, Jining, China.
authors.forename: Meng
authors.initials: M
authors.lastname: Zhang
authors.affiliation: Department of Cardiology, Affiliated hospital of Qingdao University, Qingdao, 266021, China. Electronic address: jhgjkut@sohu.com.
authors.forename: ShangLang
authors.initials: S
authors.lastname: Cai
authors.affiliation: Department of Cardiology, Fengxian Branch of Shanghai 6th People's Hospital, Shanghai 201400, China. Electronic address: jiangwma013@163.com.
authors.forename: JiangWei
authors.initials: J
authors.lastname: Ma
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000737:chemistry; D001219:Aspartate Aminotransferases / Q000378:metabolism; D002316:Cardiotonic Agents / Q000494:pharmacology*; D003402:Creatine Kinase / Q000378:metabolism; D005786:Gene Expression Regulation; D006321:Heart / Q000187:drug effects*; D053614:Janus Kinase 2 / Q000378:metabolism*; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008297:Male; D009200:Myocardial Contraction; D017202:Myocardial Ischemia / Q000473:pathology; D015428:Myocardial Reperfusion Injury / Q000378:metabolism / Q000473:pathology; D009206:Myocardium / Q000473:pathology; D009844:Oligosaccharides / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000473:pathology; D050796:STAT3 Transcription Factor / Q000378:metabolism*; D013025:Glycine max / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D002316:Cardiotonic Agents; D009844:Oligosaccharides; D050796:STAT3 Transcription Factor; C494088:Stat3 protein, rat; D007770:L-Lactate Dehydrogenase; D001219:Aspartate Aminotransferases; C507927:Jak2 protein, rat; D053614:Janus Kinase 2; D003402:Creatine Kinase
keywords: Heart; IR; JAK2/STAT3; Soybean oligosaccharides