title: Using recombinant cyanobacterium (Synechococcus elongatus) with increased carbohydrate productivity as feedstock for bioethanol production via separate hydrolysis and fermentation process.
abstract: In this work, a recombinant cyanobacterium strain with increased photosynthesis rate, cell growth and carbohydrate production efficiency was genetically engineered by co-expressing ictB, ecaA, and acsAB (encoded for bacterial cellulose) in Synechococcus elongatus PCC7942. The resulting cyanobacterial biomass could be effectively hydrolyzed with dilute acid (2% sulfuric acid), achieving a nearly 90% glucose recovery at a biomass concentration of 80 g/L. Bioethanol can be produced from fermenting the acidic hydrolysate of S. elongatus PCC7942 via separate hydrolysis and fermentation (SHF) process at a concentration of 7.2 g/L and with a 91% theoretical yield.
authors.affiliation: Department of Biotechnology, Fooyin University, Kaohsiung, Taiwan.
authors.forename: Te-Jin
authors.initials: TJ
authors.lastname: Chow
authors.affiliation: Department of Biotechnology, Fooyin University, Kaohsiung, Taiwan; Doctoral Degree Program in Marine Biotechnology, National Sun Yat-Sen University, Kaohsiung, Taiwan.
authors.forename: Hsiang-Yen
authors.initials: HY
authors.lastname: Su
authors.affiliation: Department of Chemical Engineering, National Cheng Kung University, Tainan, Taiwan.
authors.forename: Tsung-Yu
authors.initials: TY
authors.lastname: Tsai
authors.affiliation: Department of Biotechnology, Fooyin University, Kaohsiung, Taiwan.
authors.forename: Hsiang-Hui
authors.initials: HH
authors.lastname: Chou
authors.affiliation: Doctoral Degree Program in Marine Biotechnology, National Sun Yat-Sen University, Kaohsiung, Taiwan; Department of Marine Biotechnology and Resources, National Sun Yat-Sen University, Kaohsiung, Taiwan.
authors.forename: Tse-Min
authors.initials: TM
authors.lastname: Lee
authors.affiliation: Department of Chemical Engineering, National Cheng Kung University, Tainan, Taiwan; Center for Biosciences and Biotechnology, National Cheng Kung University, Tainan, Taiwan; Research Center for Energy Technology and Strategy, National Cheng Kung University, Tainan, Taiwan. Electronic address: changjs@mail.ncku.edu.tw.
authors.forename: Jo-Shu
authors.initials: JS
authors.lastname: Chang
mesh_terms: D056804:Biofuels / Q000382:microbiology*; D018533:Biomass; D001709:Biotechnology / Q000379:methods; D002241:Carbohydrates / Q000096:biosynthesis*; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation / Q000187:drug effects; D005798:Genes, Bacterial; D005947:Glucose / Q000378:metabolism; D006868:Hydrolysis; D010788:Photosynthesis / Q000187:drug effects; D016133:Polymerase Chain Reaction; D011995:Recombination, Genetic / Q000235:genetics*; D013464:Sulfuric Acids / Q000494:pharmacology; D046940:Synechococcus / Q000187:drug effects / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D014170:Transformation, Genetic / Q000187:drug effects
chemical_list: D056804:Biofuels; D002241:Carbohydrates; D013464:Sulfuric Acids; D000431:Ethanol; D005947:Glucose; C033158:sulfuric acid
keywords: Bioethanol; Carbohydrate; Cellulose; Recombinant cyanobacterium; Synechococcus elongatus PCC7942