title: Synthesis and characterisation of morin reduced gold nanoparticles and its cytotoxicity in MCF-7 cells.
abstract: There is significant interest in investigating the therapeutic potential of phytochemical reduced and bound gold nanoparticles (AuNPs) as it bridges the gap between nanotechnology and therapy. In the present study, AuNPs prepared using the flavonoid morin (mAuNPs) are characterised and have been studied for their anti-cancer effects. The -OH groups of morin reduce Au(3+) and stabilize Au(0) to form spherical and crystalline mAuNPs. These mAuNPs are biocompatible towards normal human blood cells and breast epithelial cells. Through TEM analysis, we report that they are readily taken up by breast cancer cells (MCF-7) to induce cell death. Apoptosis has also been assessed by other morphological observations and cell viability studies. Flow cytometric studies reveal that the cells undergo a transient phase of apoptosis progressing towards secondary necrosis as the dose and time of mAuNPs treatment increases. The ability of mAuNPs to induce cell death in MCF-7 cells indicates its potential as an anti-cancer agent.
authors.affiliation: Biochemistry Laboratory, Central Leather Research Institute, Adyar, Chennai, India.
authors.forename: Sindhu
authors.initials: S
authors.lastname: Kondath
authors.affiliation: Biochemistry Laboratory, Central Leather Research Institute, Adyar, Chennai, India.
authors.forename: Bhuvanasree
authors.initials: B
authors.lastname: Srinivas Raghavan
authors.affiliation: Bio-Physics Laboratory, Central Leather Research Institute, Adyar, Chennai, India.
authors.forename: Rajaram
authors.initials: R
authors.lastname: Anantanarayanan
authors.affiliation: Biochemistry Laboratory, Central Leather Research Institute, Adyar, Chennai, India. Electronic address: rajaram.rama@gmail.com.
authors.forename: Rama
authors.initials: R
authors.lastname: Rajaram
mesh_terms: D000970:Antineoplastic Agents / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology* / Q000633:toxicity; D017209:Apoptosis / Q000187:drug effects; D001672:Biocompatible Materials / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology / Q000633:toxicity; D004912:Erythrocytes / Q000166:cytology / Q000187:drug effects; D005419:Flavonoids / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology* / Q000633:toxicity; D059565:G2 Phase Cell Cycle Checkpoints; D006046:Gold / Q000737:chemistry*; D006801:Humans; D008247:Lysosomes / Q000378:metabolism; D061986:MCF-7 Cells; D053768:Metal Nanoparticles / Q000008:administration & dosage / Q000737:chemistry* / Q000633:toxicity; D047309:Flavones
chemical_list: D000970:Antineoplastic Agents; D001672:Biocompatible Materials; D005419:Flavonoids; D006046:Gold; C008548:morin; D047309:Flavones
keywords: Apoptosis; Breast cancer; Gold nanoparticles; Morin; Nanoparticle internalization; Secondary necrosis