title: Fine mapping and conservation analysis of linear B-cell epitopes of peste des petits ruminants virus nucleoprotein.
abstract: Nucleoprotein (NP) is the most abundant and highly immunogenic protein of morbillivirus, and is presently the basis of most diagnostic assays for peste des petits ruminants virus (PPRV). In this study, fine epitope mapping and conservation analysis of linear B-cell epitopes on the PPRV NP has been undertaken using biosynthetic peptides. Nineteen linear B-cell epitopes were identified and their corresponding minimal motifs were located on the NP of PPRV China/Tibet/Geg/07-30. Conservation analysis indicated that ten of the 19 minimal motifs were conserved among 46 PPRV strains. Peptides containing the minimal motifs were recognized using anti-PPRV serum from a goat immunized with PPRV vaccine strain Nigeria 75/1. Identified epitopes and their motifs improve our understanding of the antigenic characteristics of PPRV NP and provide a basis for the development of epitope-based diagnostic assays.
authors.affiliation: Institute of Animal Science and Veterinary Medicine, Shanghai Key Laboratory of Agricultural Genetics and Breeding, Shanghai Academy of Agricultural Sciences (SAAS), Shanghai 201106, China.
authors.forename: Ruisong
authors.initials: R
authors.lastname: Yu
authors.affiliation: Institute of Animal Science and Veterinary Medicine, Shanghai Key Laboratory of Agricultural Genetics and Breeding, Shanghai Academy of Agricultural Sciences (SAAS), Shanghai 201106, China; College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Taigu, Shanxi 030801, China.
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Fan
authors.affiliation: Department of Reproductive Biology, Shanghai Institute of Planned Parenthood Research, Shanghai 200032, China.
authors.forename: Wanxiang
authors.initials: W
authors.lastname: Xu
authors.affiliation: Institute of Animal Science and Veterinary Medicine, Shanghai Key Laboratory of Agricultural Genetics and Breeding, Shanghai Academy of Agricultural Sciences (SAAS), Shanghai 201106, China.
authors.forename: Wentao
authors.initials: W
authors.lastname: Li
authors.affiliation: Institute of Animal Science and Veterinary Medicine, Shanghai Key Laboratory of Agricultural Genetics and Breeding, Shanghai Academy of Agricultural Sciences (SAAS), Shanghai 201106, China.
authors.forename: Shijuan
authors.initials: S
authors.lastname: Dong
authors.affiliation: Institute of Animal Science and Veterinary Medicine, Shanghai Key Laboratory of Agricultural Genetics and Breeding, Shanghai Academy of Agricultural Sciences (SAAS), Shanghai 201106, China.
authors.forename: Yumin
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: Department of Reproductive Biology, Shanghai Institute of Planned Parenthood Research, Shanghai 200032, China.
authors.forename: Yaping
authors.initials: Y
authors.lastname: He
authors.affiliation: Department of Reproductive Biology, Shanghai Institute of Planned Parenthood Research, Shanghai 200032, China.
authors.forename: Haiping
authors.initials: H
authors.lastname: Tang
authors.affiliation: College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Taigu, Shanxi 030801, China. Electronic address: drdurong@163.com.
authors.forename: Rong
authors.initials: R
authors.lastname: Du
authors.affiliation: Institute of Animal Science and Veterinary Medicine, Shanghai Key Laboratory of Agricultural Genetics and Breeding, Shanghai Academy of Agricultural Sciences (SAAS), Shanghai 201106, China. Electronic address: zhenli60@163.com.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Li
mesh_terms: D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D000818:Animals; D018604:Epitope Mapping / Q000662:veterinary; D018985:Epitopes, B-Lymphocyte / Q000276:immunology*; D005260:Female; D015511:Goat Diseases / Q000276:immunology / Q000517:prevention & control* / Q000821:virology; D006041:Goats / Q000276:immunology*; D008969:Molecular Sequence Data; D009698:Nucleoproteins / Q000276:immunology; D029021:Peste-des-Petits-Ruminants / Q000175:diagnosis / Q000821:virology*; D018107:Peste-des-petits-ruminants virus / Q000276:immunology*; D011817:Rabbits; D011994:Recombinant Proteins; D014611:Vaccination / Q000662:veterinary*; D014764:Viral Proteins / Q000276:immunology
chemical_list: D018985:Epitopes, B-Lymphocyte; D009698:Nucleoproteins; D011994:Recombinant Proteins; D014764:Viral Proteins
keywords: Linear epitope; Mapping; Minimal motif; Nucleoprotein; Peste des petits ruminants virus