title: Structural and biochemical characterization of a carbohydrate acetylesterase from Sinorhizobium meliloti 1021.
abstract: In many microorganisms, carbohydrate acetylesterases remove the acetyl groups from various types of carbohydrates. Sm23 from Sinorhizobium meliloti is a putative member of carbohydrate esterase family 3 (CE3) in the CAZy classification system. Here, we determined the crystal structure of Sm23 at 1.75 Å resolution and investigated functional properties using biochemical methods. Furthermore, immobilized Sm23 exhibited improved stability compared with soluble Sm23, which can be used for the design of plant cell wall degrading-systems.
authors.affiliation: Department of Molecular Cell Biology, Samsung Biomedical Research Institute, Sungkyunkwan University School of Medicine, Suwon 440-746, Republic of Korea.
authors.forename: Kyungmin
authors.initials: K
authors.lastname: Kim
authors.affiliation: Department of Applied Chemistry and Biological Engineering, College of Engineering, Ajou University, Suwon 443-741, Republic of Korea.
authors.forename: Bum Han
authors.initials: BH
authors.lastname: Ryu
authors.affiliation: Department of Molecular Cell Biology, Samsung Biomedical Research Institute, Sungkyunkwan University School of Medicine, Suwon 440-746, Republic of Korea.
authors.forename: Sung Soo
authors.initials: SS
authors.lastname: Kim
authors.affiliation: Department of Applied Chemistry and Biological Engineering, College of Engineering, Ajou University, Suwon 443-741, Republic of Korea.
authors.forename: Deu Rae
authors.initials: DR
authors.lastname: An
authors.affiliation: Department of Molecular Cell Biology, Samsung Biomedical Research Institute, Sungkyunkwan University School of Medicine, Suwon 440-746, Republic of Korea.
authors.forename: Tri Duc
authors.initials: TD
authors.lastname: Ngo
authors.affiliation: Department of Molecular Cell Biology, Samsung Biomedical Research Institute, Sungkyunkwan University School of Medicine, Suwon 440-746, Republic of Korea.
authors.forename: Ramesh
authors.initials: R
authors.lastname: Pandian
authors.affiliation: Department of Molecular Cell Biology, Samsung Biomedical Research Institute, Sungkyunkwan University School of Medicine, Suwon 440-746, Republic of Korea. Electronic address: kyeongkyu@skku.edu.
authors.forename: Kyeong Kyu
authors.initials: KK
authors.lastname: Kim
authors.affiliation: Department of Applied Chemistry and Biological Engineering, College of Engineering, Ajou University, Suwon 443-741, Republic of Korea. Electronic address: doohunkim@ajou.ac.kr.
authors.forename: T Doohun
authors.initials: TD
authors.lastname: Kim
mesh_terms: D000115:Acetylesterase / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D018360:Crystallography, X-Ray; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D016962:Sinorhizobium meliloti / Q000201:enzymology* / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D004800:Enzymes, Immobilized; D000115:Acetylesterase
keywords: Acetylation; Plant cell wall; Rhizobium species