title: Quartz crystal microbalance detection of protein amplified by nicked circling, rolling circle amplification and biocatalytic precipitation.
abstract: A novel quartz crystal microbalance (QCM) assay was described for sensitive detection of protein. Lysozyme was used as a model of protein. To enhance the sensitivity of this QCM biosensor, biocatalytic precipitation (BCP) reaction combined with strand-scission cycle and rolling circle amplification (RCA) were applied together for the first time. As a result of the multi-signal amplification in this aptasensor, the detection limit for lysozyme was down to 0.3fM. What is more, this amplified QCM biosensor also showed good selectivity and practical usage in human serum.
authors.affiliation: Key Laboratory of Sensor Analysis of Tumor Marker, Ministry of Education, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, PR China.
authors.forename: Xiaoru
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Sensor Analysis of Tumor Marker, Ministry of Education, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, PR China.
authors.forename: Jiao
authors.initials: J
authors.lastname: Chen
authors.affiliation: Key Laboratory of Sensor Analysis of Tumor Marker, Ministry of Education, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, PR China.
authors.forename: Hongxia
authors.initials: H
authors.lastname: Liu
authors.affiliation: Key Laboratory of Detection Technology of Shandong Province for Tumor Marker, College of Chemistry and Chemical Engineering, Linyi University, Linyi 276005, PR China. Electronic address: shushzhang@126.com.
authors.forename: Shusheng
authors.initials: S
authors.lastname: Zhang
mesh_terms: D000818:Animals; D052157:Aptamers, Nucleotide / Q000737:chemistry* / Q000235:genetics; D055162:Biocatalysis; D015374:Biosensing Techniques / Q000379:methods*; D006801:Humans; D057230:Limit of Detection; D009113:Muramidase / Q000032:analysis / Q000097:blood*; D021141:Nucleic Acid Amplification Techniques / Q000379:methods; D058525:Quartz Crystal Microbalance Techniques / Q000379:methods*
chemical_list: D052157:Aptamers, Nucleotide; D009113:Muramidase
keywords: Biocatalytic precipitation; Lysozyme; Quartz crystal microbalance; Rolling circle amplification