title: A natural phenylpropionate derivative from Mirabilis himalaica inhibits cell proliferation and induces apoptosis in HepG2 cells.
abstract: Bioactivity-guided study led to the isolation of a natural phenylpropionate derivative, (E)-3-(4-hydroxy-2-methoxyphenyl)-propenoic acid 4-hydroxy-3-methoxyphenyl ester from the roots of Mirabilis himalaica. Cellular analysis showed that compound 1 specifically inhibited the cancer cell growth through the S phase arrest. Mechanistically, compound 1 was able to induce the apoptosis in HepG2 cells through mitochondrial apoptosis pathway in which Bcl-2 and p53 were required. Interestingly, the cellular phenotype of compound 1 were shown specifically in cancer cells originated from hepatocellular carcinoma (HepG2) while compromised influence by compound 1 were detected within the normal human liver cells (L-02). Consistently, the in vivo inhibitory effects of compound 1 on tumor growth were validated by the in xenograft administrated with HepG2 cells. Our results provided a novel compound which might serve as a promising candidate and shed light on the therapy of the hepatocellular carcinoma.
authors.affiliation: Key Laboratory on Luminescence and Real-Time Analysis (Ministry of Education), College of Pharmaceutical Sciences, Southwest University, Chongqing 400715, China.
authors.forename: LingHu
authors.initials: L
authors.lastname: Lang
authors.affiliation: State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400715, China; School of Life Sciences, Southwest University, Chongqing 400715, China.
authors.forename: Shunqin
authors.initials: S
authors.lastname: Zhu
authors.affiliation: School of Life Sciences, Southwest University, Chongqing 400715, China.
authors.forename: Haoxing
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Key Laboratory on Luminescence and Real-Time Analysis (Ministry of Education), College of Pharmaceutical Sciences, Southwest University, Chongqing 400715, China.
authors.forename: Panpan
authors.initials: P
authors.lastname: Yang
authors.affiliation: Key Laboratory on Luminescence and Real-Time Analysis (Ministry of Education), College of Pharmaceutical Sciences, Southwest University, Chongqing 400715, China.
authors.forename: Haixia
authors.initials: H
authors.lastname: Fan
authors.affiliation: Key Laboratory on Luminescence and Real-Time Analysis (Ministry of Education), College of Pharmaceutical Sciences, Southwest University, Chongqing 400715, China.
authors.forename: Shanlin
authors.initials: S
authors.lastname: Li
authors.affiliation: School of Life Sciences, Southwest University, Chongqing 400715, China.
authors.forename: Zhihua
authors.initials: Z
authors.lastname: Liao
authors.affiliation: Tibetan Collaborative Innovation Center of Agricultural and Animal Husbandry Resources, Agriculture and Animal Husbandry College, Tibet University, Nyingchi, Tibet 860000, China.
authors.forename: Xiaozhong
authors.initials: X
authors.lastname: Lan
authors.affiliation: State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400715, China. Electronic address: hongjuan.cui@gmail.com.
authors.forename: Hongjuan
authors.initials: H
authors.lastname: Cui
authors.affiliation: Key Laboratory on Luminescence and Real-Time Analysis (Ministry of Education), College of Pharmaceutical Sciences, Southwest University, Chongqing 400715, China. Electronic address: mminchen@swu.edu.cn.
authors.forename: Min
authors.initials: M
authors.lastname: Chen
mesh_terms: D017209:Apoptosis; D001688:Biological Products; D006528:Carcinoma, Hepatocellular / Q000378:metabolism* / Q000473:pathology; D049109:Cell Proliferation; D056945:Hep G2 Cells; D006801:Humans; D008113:Liver Neoplasms / Q000378:metabolism* / Q000473:pathology; D039282:Mirabilis / Q000378:metabolism*; D010666:Phenylpropionates / Q000494:pharmacology*
chemical_list: D001688:Biological Products; D010666:Phenylpropionates
keywords: Apoptosis; Cell cycle; Cell proliferation; HepG2; Mirabilis himalaica