title: High purity of human oligodendrocyte progenitor cells obtained from neural stem cells: suitable for clinical application.
abstract: BACKGROUND
  Recent studies have suggested that the transplantation of oligodendrocyte progenitor cells (OPCs) may be a promising potential therapeutic strategy for a broad range of diseases affecting myelin, such as multiple sclerosis, periventricular leukomalacia, and spinal cord injury. Clinical interest arose from the potential of human stem cells to be directed to OPCs for the clinical application of treating these diseases since large quantities of high quality OPCs are needed. However, to date, there have been precious few studies about OPC induction from human neural stem cells (NSCs).
  
  
  METHODS
  Here we successfully directed human fetal NSCs into highly pure OPCs using a cocktail of basic fibroblast growth factor, platelet-derived growth factor, and neurotrophic factor-3.
  
  
  RESULTS
  These cells had typical morphology of OPCs, and 80-90% of them expressed specific OPC markers such as A2B5, O4, Sox10 and PDGF-αR. When exposed to differentiation medium, 90% of the cells differentiated into oligodendrocytes. The OPCs could be amplified in our culture medium and passaged at least 10 times.
  Compared to a recent published method, this protocol had much higher stability and repeatability, and OPCs could be obtained from NSCs from passage 5 to 38. It also obtained more highly pure OPCs (80-90%) via simpler and more convenient manipulation.
  
  
  CONCLUSIONS
  This study provided an easy and efficient method to obtain large quantities of high-quality human OPCs to meet clinical demand.
authors.affiliation: Department of Pediatrics, Navy General Hospital, NO. 6, Fucheng Road, Beijing, China; Department of Pediatrics, Beijing Ditan Hospital, NO. 8, Jingshun East Street, Beijing, China.
authors.forename: Caiying
authors.initials: C
authors.lastname: Wang
authors.affiliation: Department of Pediatrics, Navy General Hospital, NO. 6, Fucheng Road, Beijing, China. Electronic address: luanzuo@yahoo.com.cn.
authors.forename: Zuo
authors.initials: Z
authors.lastname: Luan
authors.affiliation: Department of Pediatrics, Navy General Hospital, NO. 6, Fucheng Road, Beijing, China.
authors.forename: Yinxiang
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Department of Pediatrics, Navy General Hospital, NO. 6, Fucheng Road, Beijing, China.
authors.forename: Zhaoyan
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Department of Pediatrics, Navy General Hospital, NO. 6, Fucheng Road, Beijing, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Department of Pediatrics, Navy General Hospital, NO. 6, Fucheng Road, Beijing, China.
authors.forename: Yabin
authors.initials: Y
authors.lastname: Lu
authors.affiliation: Department of Pediatrics, Navy General Hospital, NO. 6, Fucheng Road, Beijing, China.
authors.forename: Qingan
authors.initials: Q
authors.lastname: Du
mesh_terms: D018929:Cell Culture Techniques; D002454:Cell Differentiation; D002478:Cells, Cultured; D003470:Culture Media; D053595:Embryonic Stem Cells / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology; D006133:Growth Substances / Q000494:pharmacology; D006801:Humans; D007150:Immunohistochemistry; D058953:Neural Stem Cells / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D020933:Neurotrophin 3 / Q000494:pharmacology; D009836:Oligodendroglia / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology; D020796:Receptor, Platelet-Derived Growth Factor alpha / Q000378:metabolism; D055757:SOXE Transcription Factors / Q000378:metabolism
chemical_list: D003470:Culture Media; D006133:Growth Substances; D020933:Neurotrophin 3; D010982:Platelet-Derived Growth Factor; C527923:SOX10 protein, human; D055757:SOXE Transcription Factors; D016222:Fibroblast Growth Factor 2; D020796:Receptor, Platelet-Derived Growth Factor alpha
keywords: Clinical application; Neural stem cells; Oligodendrocyte progenitor cell