title: Detection of microRNA in clinical tumor samples by isothermal enzyme-free amplification and label-free graphene oxide-based SYBR Green I fluorescence platform.
abstract: MicroRNAs (miRNAs) are a kind of small molecules that involve in many important life activities. They have higher expression levels in many kinds of cancers. In this study, we developed an isothermal enzyme-free amplification (EFA) and label-free graphene oxide (GO)-based SYBR Green I fluorescence platform for detection of miRNA. MiRNA-21 was used as an example to demonstrate the feasibility of the method. Results show that the sensitivity of miRNA-21 is 1pM, and the linearity range is from 1pM to 1nM. The method can specifically discriminate miRNA-21 from miRNA-210 and miRNA-214. Three tumor cell lines of A549, HepG2 and MCF7 were detected by the method. The sensitivities of them were 10(2) cells, 10(3) cells and 10(3) cells respectively. Clinical tumor samples were also tested by this method, and 29 of 40 samples gave out positive signals. The method holds great promise in miRNA detection due to its convenience, rapidness, inexpensive and specificity.
authors.affiliation: School of Chemistry and Environment, South China Normal University, Guangzhou 510006, China. Electronic address: zhudb@scnu.edu.cn.
authors.forename: Debin
authors.initials: D
authors.lastname: Zhu
authors.affiliation: Department of Medical Genetics and Cell Biology, Guangzhou Medical University, Guangzhou 510182, China.
authors.forename: Lan
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Guangzhou Nansha Center for Disease Control & Prevention, Guangzhou 511457, China.
authors.forename: Wenge
authors.initials: W
authors.lastname: Ma
authors.affiliation: Departments of Medical Ultrasonics, Third Affiliated Hospital of Sun Yat-Sen University, Guangzhou 510630, China.
authors.forename: Suqin
authors.initials: S
authors.lastname: Lu
authors.affiliation: Centre for Optical and Electromagnetic Research, South China Academy of Advanced Optoelectronics, South China Normal University, 510006 Guangzhou, China. Electronic address: xingxiaobo@scnu.edu.cn.
authors.forename: Xiaobo
authors.initials: X
authors.lastname: Xing
mesh_terms: D052160:Benzothiazoles; D015374:Biosensing Techniques / Q000379:methods; D045744:Cell Line, Tumor; D003959:Diamines; D005456:Fluorescent Dyes / Q000737:chemistry*; D006108:Graphite / Q000737:chemistry*; D006801:Humans; D057230:Limit of Detection; D035683:MicroRNAs / Q000032:analysis* / Q000235:genetics; D008958:Models, Molecular; D009369:Neoplasms / Q000235:genetics*; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D009930:Organic Chemicals / Q000737:chemistry*; D010087:Oxides / Q000737:chemistry; D011804:Quinolines; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013696:Temperature
chemical_list: D052160:Benzothiazoles; D003959:Diamines; D005456:Fluorescent Dyes; D035683:MicroRNAs; D009930:Organic Chemicals; D010087:Oxides; D011804:Quinolines; C098022:SYBR Green I; D006108:Graphite
keywords: Enzyme-free amplification; Fluorescence detection; Graphene oxide; Isothermal; Label-free; MicroRNA; SYBR Green I