title: Biological properties of a thermally crosslinked gelatin film as a novel anti-adhesive material: Relationship between the biological properties and the extent of thermal crosslinking.
abstract: In order to prevent postoperative adhesion and the related complications, a thermally crosslinked gelatin (TCG) film was developed and the basic biological properties were examined, paying special attention to the relationship between these properties and the extent of crosslinking of the film. The gelatin films crosslinked thermally for five different time periods (0, 1, 3, 8, and 14 hours) were developed and the following tests were performed. Regarding the material characterization of the films, the water content, the water solubility, and the enzymatic degradation for collagenase were found to be closely related to the duration of thermal crosslinking. In an in vitro study conducted to examine the cell growth of fibroblasts cultured on the films, the degree of cell growth, except no crosslinked film, was less than that observed in the control group, thus suggesting that such effects of the films on fibroblast cell growth may be related with their anti-adhesive effects. In in vivo tests, the films crosslinked for longer time periods (3, 8, and 14 hours) were retained for longer after being implanted into the abdominal cavity in rats and showed a significant anti-adhesive effect in the rat cecum adhesion models, indicating that the biodegradability and anti-adhesive effects of the TCG films depend on the duration of thermal crosslinking. In order to develop useful and effective anti-adhesive gelatin film, it is very important to optimize duration of the thermal crosslinking.
authors.affiliation: Division of Medical Life System, Department of Life and Medical Science, Doshisha University, Kyotanabe, Kyoto, 610-0394, Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Tsujimoto
authors.affiliation: Division of Medical Life System, Department of Life and Medical Science, Doshisha University, Kyotanabe, Kyoto, 610-0394, Japan.
authors.forename: Ayumi
authors.initials: A
authors.lastname: Tanzawa
authors.affiliation: Division of Medical Life System, Department of Life and Medical Science, Doshisha University, Kyotanabe, Kyoto, 610-0394, Japan.
authors.forename: Hiroe
authors.initials: H
authors.lastname: Miyamoto
authors.affiliation: Division of Medical Life System, Department of Life and Medical Science, Doshisha University, Kyotanabe, Kyoto, 610-0394, Japan.
authors.forename: Tsunehito
authors.initials: T
authors.lastname: Horii
authors.affiliation: Division of Medical Life System, Department of Life and Medical Science, Doshisha University, Kyotanabe, Kyoto, 610-0394, Japan.
authors.forename: Misaki
authors.initials: M
authors.lastname: Tsuji
authors.affiliation: Division of Medical Life System, Department of Life and Medical Science, Doshisha University, Kyotanabe, Kyoto, 610-0394, Japan.
authors.forename: Akari
authors.initials: A
authors.lastname: Kawasumi
authors.affiliation: Division of Medical Life System, Department of Life and Medical Science, Doshisha University, Kyotanabe, Kyoto, 610-0394, Japan.
authors.forename: Atsushi
authors.initials: A
authors.lastname: Tamura
authors.affiliation: Division of Medical Life System, Department of Life and Medical Science, Doshisha University, Kyotanabe, Kyoto, 610-0394, Japan.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Division of Medical Life System, Department of Life and Medical Science, Doshisha University, Kyotanabe, Kyoto, 610-0394, Japan.
authors.forename: Rie
authors.initials: R
authors.lastname: Abe
authors.affiliation: Division of Medical Life System, Department of Life and Medical Science, Doshisha University, Kyotanabe, Kyoto, 610-0394, Japan.
authors.forename: Shota
authors.initials: S
authors.lastname: Tanaka
authors.affiliation: Division of Medical Life System, Department of Life and Medical Science, Doshisha University, Kyotanabe, Kyoto, 610-0394, Japan.
authors.forename: Kouki
authors.initials: K
authors.lastname: Yamanaka
authors.affiliation: Division of Medical Life System, Department of Life and Medical Science, Doshisha University, Kyotanabe, Kyoto, 610-0394, Japan.
authors.forename: Mari
authors.initials: M
authors.lastname: Matoba
authors.affiliation: Division of Medical Life System, Department of Life and Medical Science, Doshisha University, Kyotanabe, Kyoto, 610-0394, Japan.
authors.forename: Hiroko
authors.initials: H
authors.lastname: Torii
authors.affiliation: Division of Medical Life System, Department of Life and Medical Science, Doshisha University, Kyotanabe, Kyoto, 610-0394, Japan.
authors.forename: Yuki
authors.initials: Y
authors.lastname: Ozamoto
authors.affiliation: Division of Medical Life System, Department of Life and Medical Science, Doshisha University, Kyotanabe, Kyoto, 610-0394, Japan.|Research and Development Department, Gunze Ltd., Ayabe, Kyoto, 623-8511, Japan.
authors.forename: Hideki
authors.initials: H
authors.lastname: Takamori
authors.affiliation: Research and Development Department, Gunze Ltd., Ayabe, Kyoto, 623-8511, Japan.|Medical Department, Nara Medical University, Kashihara, Nara, 634-8521, Japan.
authors.forename: Shuko
authors.initials: S
authors.lastname: Suzuki
authors.affiliation: Research and Development Department, Gunze Ltd., Ayabe, Kyoto, 623-8511, Japan.
authors.forename: Shinichiro
authors.initials: S
authors.lastname: Morita
authors.affiliation: Medical Department, Nara Medical University, Kashihara, Nara, 634-8521, Japan.
authors.forename: Yoshito
authors.initials: Y
authors.lastname: Ikada
authors.affiliation: Division of Medical Life System, Department of Life and Medical Science, Doshisha University, Kyotanabe, Kyoto, 610-0394, Japan.
authors.forename: Akeo
authors.initials: A
authors.lastname: Hagiwara
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D003432:Cross-Linking Reagents / Q000737:chemistry; D005260:Female; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism*; D005780:Gelatin / Q000737:chemistry / Q000494:pharmacology; D008422:Materials Testing; D008567:Membranes, Artificial; D051381:Rats; D017208:Rats, Wistar
chemical_list: D003432:Cross-Linking Reagents; D008567:Membranes, Artificial; D005780:Gelatin
keywords: anti-adhesive effect; biodegradability; gelatin film; thermal crosslinking