title: A new bioactive thiophenolic glycoside from the leaf of Massularia acuminata (g. Don Bullock) ex Hoyle (Rubiaceae).
abstract: BACKGROUND
  Massularia acuminata is a small tree or shrub of tropical rainforest. The leaves are used in Nigerian ethno-medicine for the treatment of microbial infections and pharmacological report suggested the leaf extract as possessing antioxidant activity. This study was therefore carried out to determine the most antioxidant and antimicrobial active fraction(s) of Massularia acuminata leaf and the constituent(s) responsible for the activities.
  
  
  METHODS
  The leaf of Massularia acuminata was investigated for in vitro antioxidant and antimicrobial activities, using a 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay and agar dilution method respectively.
  
  
  RESULTS
  The ethyl acetate fraction demonstrated the best activities among the partitioned fractions tested. Bioassay guided purification of the most active ethyl acetate fraction led to isolation of a new thiophenolic glycoside, characterized as 4-(3',3'-dihydroxy-1-mercaptopropyl)phenyl glycosylpyranoside.
  
  
  CONCLUSIONS
  The isolated compound from the leaf of Massularia acuminata demonstrated antioxidant and antimicrobial activities and may be responsible for the activities of leaf extract and its ethyl acetate fraction, hence this may justify its ethnomedicinal use.
authors.affiliation: Department of Pharmacognosy, Faculty of Pharmacy, Obafemi Awolowo University, Ile-Ife, Nigeria.
authors.forename: A O
authors.initials: AO
authors.lastname: Oriola
authors.affiliation: Department of Pharmacognosy, Faculty of Pharmacy, Obafemi Awolowo University, Ile-Ife, Nigeria.
authors.forename: A J
authors.initials: AJ
authors.lastname: Aladesanmi
authors.affiliation: Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Obafemi Awolowo University, Ile-Ife, Nigeria.
authors.forename: T O
authors.initials: TO
authors.lastname: Idowu
authors.affiliation: Department of Pharmaceutics, Faculty of Pharmacy, Obafemi Awolowo University, Ile-Ife, Nigeria.
authors.forename: E O
authors.initials: EO
authors.lastname: Akinkunmi
authors.affiliation: Department of Biochemistry, Faculty of Science, Obafemi Awolowo University, Ile-Ife, Nigeria.
authors.forename: E M
authors.initials: EM
authors.lastname: Obuotor
authors.affiliation: Department of Chemistry, 360 Parkers Building, University of Manitoba, Winnipeg, R3T 2N2, Canada.
authors.forename: M O
authors.initials: MO
authors.lastname: Ogunsina
mesh_terms: D000890:Anti-Infective Agents / Q000737:chemistry / Q000494:pharmacology; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects; D005658:Fungi / Q000187:drug effects; D006027:Glycosides / Q000737:chemistry / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests; D015394:Molecular Structure; D010636:Phenols / Q000737:chemistry / Q000494:pharmacology*; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry; D019666:Rubiaceae / Q000737:chemistry*; D013438:Sulfhydryl Compounds / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D000890:Anti-Infective Agents; D000975:Antioxidants; D006027:Glycosides; D010636:Phenols; D010936:Plant Extracts; D013438:Sulfhydryl Compounds; C042983:thiophenol
keywords: DPPH; Massularia acuminata, antioxidant; agar dilution; antimicrobial; thiophenolic glycoside