title: DEAD-box RNA helicase DDX3X inhibits DENV replication via regulating type one interferon pathway.
abstract: Dengue virus (DENV) is a mosquito-borne virus that threatens approximately 2.5 billion people worldwide. Vaccines against DENV are currently unavailable. DEAD-box RNA helicases (DDXs) have been reported to participate in viral replication and host innate immune response. In the present study, we analyzed the role of 40 DDX proteins during DENV replication. Among these proteins, DDX3X showed antiviral effect against DENV infection. Viral replication significantly increased in DDX3X-silenced cells compared with the controls. The interferon (IFN)-β transcription level decreased during the early stage of DENV infection in DDX3X-silenced cells compared with that in the controls. DDX3X could stimulate IFN-β transcription through the IRF3 and the NFκB branches in DENV-infected cells. Our data imply that DDX3X, a member of DEAD-box RNA helicase, is necessary for IFN production and could inhibit DENV replication.
authors.affiliation: Institute of Biology and Medical Sciences, Jiangsu Key Laboratory of Infection and Immunity, Soochow University, Suzhou City, Jiangsu Province, People's Republic of China.
authors.forename: Guanghao
authors.initials: G
authors.lastname: Li
authors.affiliation: Institute of Biology and Medical Sciences, Jiangsu Key Laboratory of Infection and Immunity, Soochow University, Suzhou City, Jiangsu Province, People's Republic of China. Electronic address: ttfeng@suda.edu.cn.
authors.forename: Tingting
authors.initials: T
authors.lastname: Feng
authors.affiliation: Institute of Biology and Medical Sciences, Jiangsu Key Laboratory of Infection and Immunity, Soochow University, Suzhou City, Jiangsu Province, People's Republic of China.
authors.forename: Wen
authors.initials: W
authors.lastname: Pan
authors.affiliation: Institute of Biology and Medical Sciences, Jiangsu Key Laboratory of Infection and Immunity, Soochow University, Suzhou City, Jiangsu Province, People's Republic of China.
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Shi
authors.affiliation: Institute of Biology and Medical Sciences, Jiangsu Key Laboratory of Infection and Immunity, Soochow University, Suzhou City, Jiangsu Province, People's Republic of China. Electronic address: daijianfeng@suda.edu.cn.
authors.forename: Jianfeng
authors.initials: J
authors.lastname: Dai
mesh_terms: D053487:DEAD-box RNA Helicases / Q000378:metabolism*; D003715:Dengue / Q000821:virology; D003716:Dengue Virus / Q000502:physiology*; D015967:Gene Expression Regulation, Viral; D020868:Gene Silencing; D057809:HEK293 Cells; D006801:Humans; D007113:Immunity, Innate; D050838:Interferon Regulatory Factor-3 / Q000378:metabolism; D007370:Interferon Type I / Q000378:metabolism*; D016328:NF-kappa B / Q000378:metabolism; D010957:Plasmids / Q000378:metabolism; D014158:Transcription, Genetic; D014779:Virus Replication
chemical_list: C494232:IRF3 protein, human; D050838:Interferon Regulatory Factor-3; D007370:Interferon Type I; D016328:NF-kappa B; C119332:DDX3X protein, human; D053487:DEAD-box RNA Helicases
keywords: Antiviral function; DDX3X; Dengue virus; Interferon; RNA helicase