title: Bis-spirolabdane diterpenoids from Leonurus japonicus and their anti-platelet aggregative activity.
abstract: Six bis-spirolabdane diterpenoids along with four known analogues were isolated from the aerial parts of Leonurus japonicus. Their structures and absolute configurations were elucidated by spectroscopic analyses, single-crystal X-ray diffraction, and a modified Mosher's method. The inhibitory activity of the compounds against the abnormal increase in platelet aggregation induced by adenosine diphosphate was investigated. Only the (13R)-bis-spirolabdane diterpenoids exhibited a significant effect.
authors.affiliation: State Key Laboratory Breeding Base of Systematic Research, Development and Utilization of Chinese Medicine Resources, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, PR China.
authors.forename: Liang
authors.initials: L
authors.lastname: Xiong
authors.affiliation: State Key Laboratory Breeding Base of Systematic Research, Development and Utilization of Chinese Medicine Resources, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, PR China.
authors.forename: Qin-Mei
authors.initials: QM
authors.lastname: Zhou
authors.affiliation: State Key Laboratory Breeding Base of Systematic Research, Development and Utilization of Chinese Medicine Resources, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, PR China. Electronic address: pengchengchengdu@126.com.
authors.forename: Cheng
authors.initials: C
authors.lastname: Peng
authors.affiliation: State Key Laboratory Breeding Base of Systematic Research, Development and Utilization of Chinese Medicine Resources, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, PR China.
authors.forename: Xiao-Fang
authors.initials: XF
authors.lastname: Xie
authors.affiliation: State Key Laboratory Breeding Base of Systematic Research, Development and Utilization of Chinese Medicine Resources, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, PR China.
authors.forename: Lu-Si
authors.initials: LS
authors.lastname: Liu
authors.affiliation: State Key Laboratory Breeding Base of Systematic Research, Development and Utilization of Chinese Medicine Resources, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, PR China.
authors.forename: Li
authors.initials: L
authors.lastname: Guo
authors.affiliation: State Key Laboratory Breeding Base of Systematic Research, Development and Utilization of Chinese Medicine Resources, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, PR China.
authors.forename: Ya-Cong
authors.initials: YC
authors.lastname: He
authors.affiliation: State Key Laboratory Breeding Base of Systematic Research, Development and Utilization of Chinese Medicine Resources, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, PR China.
authors.forename: Lian
authors.initials: L
authors.lastname: Yang
authors.affiliation: Chengdu No. 1 Pharmaceutical Co., Ltd., Chengdu 610031, PR China.
authors.forename: Zhao-Hua
authors.initials: ZH
authors.lastname: Liu
mesh_terms: D000818:Animals; D001792:Blood Platelets / Q000187:drug effects*; D004224:Diterpenes / Q000302:isolation & purification / Q000494:pharmacology*; D031332:Leonurus / Q000737:chemistry*; D015394:Molecular Structure; D035261:Plant Components, Aerial / Q000737:chemistry; D010974:Platelet Aggregation / Q000187:drug effects*; D017207:Rats, Sprague-Dawley
chemical_list: D004224:Diterpenes
keywords: Absolute configuration; Anti-platelet aggregative activity; Bis-spirolabdane diterpenoids; Leonurus japonicus