title: Identification and characterization of an anti-pseudomonal  dichlorocarbazol derivative displaying anti-biofilm activity.
abstract: Pseudomonas aeruginosa strains resistant towards all currently available antibiotics are increasingly encountered, raising the need for new anti-pseudomonal drugs. We therefore conducted a medium-throughput screen of a small-molecule collection resulting in the identification of the N-alkylated 3,6-dihalogenocarbazol 1-(sec-butylamino)-3-(3,6-dichloro-9H-carbazol-9-yl)propan-2-ol (MIC = 18.5 μg mL⁻¹). This compound, compound 1, is bacteriostatic towards a broad spectrum of Gram-positive and Gram-negative pathogens, including P. aeruginosa. Importantly, 1 also eradicates mature biofilms of P. aeruginosa. 1 displays no cytotoxicity against various human cell types, pointing to its potential for further development as a novel antibacterial drug.
authors.forename: Veerle
authors.initials: V
authors.lastname: Liebens
authors.forename: Evelien
authors.initials: E
authors.lastname: Gerits
authors.forename: Wouter J
authors.initials: WJ
authors.lastname: Knapen
authors.forename: Toon
authors.initials: T
authors.lastname: Swings
authors.forename: Serge
authors.initials: S
authors.lastname: Beullens
authors.forename: Hans P
authors.initials: HP
authors.lastname: Steenackers
authors.forename: Stijn
authors.initials: S
authors.lastname: Robijns
authors.forename: Anna
authors.initials: A
authors.lastname: Lippell
authors.forename: Alex J
authors.initials: AJ
authors.lastname: O'Neill
authors.forename: Matija
authors.initials: M
authors.lastname: Veber
authors.forename: Mirjam
authors.initials: M
authors.lastname: Fröhlich
authors.forename: Annika
authors.initials: A
authors.lastname: Krona
authors.forename: Maria
authors.initials: M
authors.lastname: Lövenklev
authors.forename: Romu
authors.initials: R
authors.lastname: Corbau
authors.forename: Arnaud
authors.initials: A
authors.lastname: Marchand
authors.forename: Patrick
authors.initials: P
authors.lastname: Chaltin
authors.forename: Katrijn
authors.initials: K
authors.lastname: De Brucker
authors.forename: Karin
authors.initials: K
authors.lastname: Thevissen
authors.forename: Bruno P
authors.initials: BP
authors.lastname: Cammue
authors.forename: Maarten
authors.initials: M
authors.lastname: Fauvart
authors.forename: Natalie
authors.initials: N
authors.lastname: Verstraeten
authors.forename: Jan
authors.initials: J
authors.lastname: Michiels
mesh_terms: D000900:Anti-Bacterial Agents / Q000627:therapeutic use*; D018441:Biofilms; D002227:Carbazoles / Q000032:analysis / Q000737:chemistry*; D006801:Humans; D008826:Microbial Sensitivity Tests; D011550:Pseudomonas aeruginosa / Q000302:isolation & purification*
chemical_list: D000900:Anti-Bacterial Agents; D002227:Carbazoles