title: Caveolin expression changes in the neurovascular unit after juvenile traumatic brain injury: signs of blood-brain barrier healing?
abstract: Traumatic brain injury (TBI) is one of the major causes of death and disability in pediatrics, and results in a complex cascade of events including the disruption of the blood-brain barrier (BBB). A controlled-cortical impact on post-natal 17-day-old rats induced BBB disruption by IgG extravasation from 1 to 3 days after injury and returned to normal at day 7. In parallel, we characterized the expression of three caveolin isoforms, caveolin 1 (cav-1), caveolin 2 (cav-2) and caveolin 3 (cav-3). While cav-1 and cav-2 are expressed on endothelial cells, both cav-1 and cav-3 were found to be present on reactive astrocytes, in vivo and in vitro. Following TBI, cav-1 expression was increased in blood vessels at 1 and 7 days in the perilesional cortex. An increase of vascular cav-2 expression was observed 7 days after TBI. In contrast, astrocytic cav-3 expression decreased 3 and 7 days after TBI. Activation of endothelial nitric oxide synthase (eNOS) (via its phosphorylation) was detected 1 day after TBI and phospho-eNOS was detected both in association with blood vessels and with astrocytes. The molecular changes involving caveolins occurring in endothelial cells following juvenile-TBI might participate, independently of eNOS activation, to a mechanism of BBB repair while, they might subserve other undefined roles in astrocytes.
authors.affiliation: CNRS UMR5287, University of Bordeaux, Bordeaux, France; Department of Physiology, Loma Linda University, Loma Linda, CA, USA; Department of Pediatrics, Loma Linda University, Loma Linda, CA, USA. Electronic address: Jerome.badaut@u-bordeaux.fr.
authors.forename: J
authors.initials: J
authors.lastname: Badaut
authors.affiliation: Department of Physiology, Loma Linda University, Loma Linda, CA, USA.
authors.forename: D O
authors.initials: DO
authors.lastname: Ajao
authors.affiliation: Department of Physiology, Loma Linda University, Loma Linda, CA, USA; Department of Pediatrics, Loma Linda University, Loma Linda, CA, USA.
authors.forename: D W
authors.initials: DW
authors.lastname: Sorensen
authors.affiliation: Department of Physiology, Loma Linda University, Loma Linda, CA, USA.
authors.forename: A M
authors.initials: AM
authors.lastname: Fukuda
authors.affiliation: Department of Physiology, University of Lausanne, Lausanne, Switzerland.
authors.forename: L
authors.initials: L
authors.lastname: Pellerin
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000378:metabolism / Q000473:pathology; D001812:Blood-Brain Barrier / Q000378:metabolism* / Q000473:pathology; D001921:Brain / Q000098:blood supply / Q000254:growth & development / Q000378:metabolism* / Q000473:pathology; D001930:Brain Injuries / Q000378:metabolism* / Q000473:pathology; D051242:Caveolin 1 / Q000378:metabolism*; D051243:Caveolin 2 / Q000378:metabolism*; D051244:Caveolin 3 / Q000378:metabolism*; D002478:Cells, Cultured; D004195:Disease Models, Animal; D004730:Endothelium, Vascular / Q000378:metabolism / Q000473:pathology; D008297:Male; D052250:Nitric Oxide Synthase Type III / Q000378:metabolism; D010766:Phosphorylation; D017207:Rats, Sprague-Dawley; D013997:Time Factors
chemical_list: C495537:Cav1 protein, rat; C495546:Cav2 protein, rat; C495549:Cav3 protein, rat; D051242:Caveolin 1; D051243:Caveolin 2; D051244:Caveolin 3; D052250:Nitric Oxide Synthase Type III; C496316:Nos3 protein, rat
keywords: astrocyte; blood–brain barrier; caveolin; endothelium; juvenile traumatic brain injury