title: High performance liquid chromatography coupled with resonance Rayleigh scattering for the detection of three fluoroquinolones and mechanism study.
abstract: A reliable and versatile high performance liquid chromatography coupled with resonance Rayleigh scattering method was established for the determination of three fluoroquinolones, including levofloxacin, norfloxacin and enrofloxacin in water sample and human urine sample. In pH 4.4-4.6 Britton-Robinson buffer medium, the fluoroquinolones separated by high performance liquid chromatography could react with erythrosine to form 1:1 ion-association complexes, which could make contributions to the great enhancement of RRS. The resonance Rayleigh scattering signal was recorded at λex=λem=330 nm. The resonance Rayleigh scattering spectral characteristics of the drugs and the experimental conditions such as pH, detection wavelength, erythrosine concentration, flow rate, the length of reaction tube were studied. Quantum chemistry calculation, Fourier transform infrared spectroscopy and absorption spectroscopy were used to discuss the reaction mechanism. The recoveries of samples added standard ranged from 97.53% to 102.00%, and the relative standard deviation was below 4.64%. The limit of detection (S/N=3) of 0.05-0.12 μg mL(-1) was reached, and the linear regression coefficients were all above 0.999. The proposed method was proved as a simple, low cost and high sensitivity method.
authors.affiliation: Chemistry and Chemical Engineering, Southwest University, No. 2 Tiansheng Road, Beibei District, Chongqing 400715, PR China.
authors.forename: Mingqiong
authors.initials: M
authors.lastname: Zhou
authors.affiliation: Chemistry and Chemical Engineering, Southwest University, No. 2 Tiansheng Road, Beibei District, Chongqing 400715, PR China. Electronic address: hxpengjd@swu.edu.cn.
authors.forename: Jingdong
authors.initials: J
authors.lastname: Peng
authors.affiliation: Chemistry and Chemical Engineering, Southwest University, No. 2 Tiansheng Road, Beibei District, Chongqing 400715, PR China.
authors.forename: Rongxing
authors.initials: R
authors.lastname: He
authors.affiliation: Chemistry and Chemical Engineering, Southwest University, No. 2 Tiansheng Road, Beibei District, Chongqing 400715, PR China.
authors.forename: Yuting
authors.initials: Y
authors.lastname: He
authors.affiliation: Chemistry and Chemical Engineering, Southwest University, No. 2 Tiansheng Road, Beibei District, Chongqing 400715, PR China.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Chemistry and Chemical Engineering, Southwest University, No. 2 Tiansheng Road, Beibei District, Chongqing 400715, PR China.
authors.forename: Aiping
authors.initials: A
authors.lastname: Li
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D000077422:Enrofloxacin; D004923:Erythrosine / Q000737:chemistry; D024841:Fluoroquinolones / Q000032:analysis / Q000737:chemistry / Q000652:urine*; D006863:Hydrogen-Ion Concentration; D064704:Levofloxacin / Q000737:chemistry / Q000652:urine; D008027:Light; D008958:Models, Molecular; D009643:Norfloxacin / Q000737:chemistry / Q000652:urine; D011789:Quantum Theory; D012015:Reference Standards; D012542:Scattering, Radiation; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D024841:Fluoroquinolones; D000077422:Enrofloxacin; D064704:Levofloxacin; D009643:Norfloxacin; D004923:Erythrosine
keywords: Erythrosine; Fluoroquinolones; High performance liquid chromatography; Resonance Rayleigh scattering