title: Impact of diamond nanoparticles on neural cells.
abstract: Diamond nanoparticles (DNPs) are very attractive for biomedical applications, particularly for bioimaging. The aim of this study was to evaluate the impact of DNPs on neural cancer cells and thus to assess the possible application of DNPs for these cells imaging. For this purpose, the neuroblastoma SH-SY5Y cell line was chosen. Cells were cultured in medium with different concentrations (15, 50, 100 and 150 μg/ml) of DNPs. After 48 h of incubation, cell metabolic activity was evaluated by the XTT assay. For assessment of cellular metabolic activity, cells were also cultured on differently terminated nanocrystalline diamond (NCD) coatings in medium with 150 μg/ml of DNPs. Cell adhesion and morphology were evaluated by brightfield microscopy. Diamond nanoparticle internalization was determined by confocal microscopy. The obtained results showed that low concentrations (15, 50 and 100 μg/ml) of nanoparticles did not significantly affect the SH-SY5Y cell metabolic activity. However, a higher concentration (150 μg/ml) of DNPs statistically significantly reduced SH-SY5Y cell metabolic activity. After 48 h incubation with 150 μg/ml DNPs, cell metabolic activity was 23% lower than in medium without DNPs on standard tissue culture polystyrene.
authors.affiliation: Department of Physical Chemistry, Faculty of Chemistry, Vilnius University, Naugarduko 24, LT-03225 Vilnius, Lithuania; Department of Stem Cell Biology, State Research Institute Centre for Innovative Medicine, Zygimantu 9, LT-01102 Vilnius, Lithuania.
authors.forename: Aida
authors.initials: A
authors.lastname: Vaitkuviene
authors.affiliation: Department of Physical Chemistry, Faculty of Chemistry, Vilnius University, Naugarduko 24, LT-03225 Vilnius, Lithuania.
authors.forename: Vilma
authors.initials: V
authors.lastname: Ratautaite
authors.affiliation: NanoTechnas-Center of Nanotechnology and Material Science, Faculty of Chemistry, Vilnius University, Naugarduko 24, LT-03225 Vilnius, Lithuania.
authors.forename: Almira
authors.initials: A
authors.lastname: Ramanaviciene
authors.affiliation: Hasselt University, Biomedical Research Institute, Agoralaan Building C, Diepenbeek B-3590, Belgium.
authors.forename: Kathleen
authors.initials: K
authors.lastname: Sanen
authors.affiliation: Hasselt University, Biomedical Research Institute, Agoralaan Building C, Diepenbeek B-3590, Belgium.
authors.forename: Rik
authors.initials: R
authors.lastname: Paesen
authors.affiliation: Hasselt University, Biomedical Research Institute, Agoralaan Building C, Diepenbeek B-3590, Belgium.
authors.forename: Marcel
authors.initials: M
authors.lastname: Ameloot
authors.affiliation: Czech Technical University in Prague, Faculty of Biomedical Engineering, Sítná sq. 3105, 272 01 Kladno, Czech Republic.
authors.forename: Vladimira
authors.initials: V
authors.lastname: Petrakova
authors.affiliation: Hasselt University, Institute for Materials Research, Division IMOMEC, Wetenschapspark 1, B-3590 Diepenbeek, Belgium.
authors.forename: Matthew
authors.initials: M
authors.lastname: McDonald
authors.affiliation: Hasselt University, Institute for Materials Research, Division IMOMEC, Wetenschapspark 1, B-3590 Diepenbeek, Belgium.
authors.forename: Farnoosh
authors.initials: F
authors.lastname: Vahidpour
authors.affiliation: Department of Stem Cell Biology, State Research Institute Centre for Innovative Medicine, Zygimantu 9, LT-01102 Vilnius, Lithuania.
authors.forename: Vytautas
authors.initials: V
authors.lastname: Kaseta
authors.affiliation: Department of Stem Cell Biology, State Research Institute Centre for Innovative Medicine, Zygimantu 9, LT-01102 Vilnius, Lithuania.
authors.forename: Giedre
authors.initials: G
authors.lastname: Ramanauskaite
authors.affiliation: Department of Stem Cell Biology, State Research Institute Centre for Innovative Medicine, Zygimantu 9, LT-01102 Vilnius, Lithuania.
authors.forename: Gene
authors.initials: G
authors.lastname: Biziuleviciene
authors.affiliation: Hasselt University, Biomedical Research Institute, Agoralaan Building C, Diepenbeek B-3590, Belgium; Hasselt University, Institute for Materials Research, Division IMOMEC, Wetenschapspark 1, B-3590 Diepenbeek, Belgium.
authors.forename: Milos
authors.initials: M
authors.lastname: Nesladek
authors.affiliation: Department of Physical Chemistry, Faculty of Chemistry, Vilnius University, Naugarduko 24, LT-03225 Vilnius, Lithuania; Laboratory of NanoBioTechnology, Department of Materials Science and Electronics, Institute of Semiconductor Physics, State Scientific Research Institute Centre for Physical Sciences and Technology, A. Gostauto 11, LT-01108 Vilnius, Lithuania. Electronic address: Arunas.Ramanavicius@chf.vu.lt.
authors.forename: Arunas
authors.initials: A
authors.lastname: Ramanavicius
mesh_terms: D002448:Cell Adhesion / Q000187:drug effects; D018929:Cell Culture Techniques; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D020099:Coated Materials, Biocompatible / Q000138:chemical synthesis / Q000494:pharmacology*; D006801:Humans; D058612:Nanodiamonds / Q000737:chemistry*; D009447:Neuroblastoma / Q000473:pathology*
chemical_list: D020099:Coated Materials, Biocompatible; D058612:Nanodiamonds
keywords: Cytotoxicity/biocompatibility; Diamond nanoparticles; Nanocrystalline diamond coating; Neuroblastoma SH-SY5Y cell line