title: Recycling microbial lipid production wastes to cultivate oleaginous yeasts.
abstract: To reduce wastes and the costs of microbial lipid production, it is imperative to recycle resources, including spent cell mass, mineral nutrients and water. In the present study, lipid production by the oleaginous yeast Rhodosporidium toruloides was used as a model system to demonstrate resources recycling. It was found that the hydrolysates of spent cell mass were good media to support cell growth of various oleaginous yeasts. When serial repitching experiments were performed using 70g/L glucose and the hydrolysates alone as nutrients, it produced 16.6, 14.6 and 12.9g/L lipids, for three successive cycles, while lipid titre remained almost constant when spent water was also recycled. The cell mass hydrolysates could be used as equivalents to the mixture of yeast extract and peptone to support lipid production from corn stalk hydrolysates. Our results showed efficient recycling of lipid production wastes and should be helpful to advance microbial lipid technology.
authors.affiliation: Division of Biotechnology and Dalian National Laboratory for Clean Energy, Dalian Institute of Chemical Physics, CAS, Dalian 116023, China; School of Life Science and Biotechnology, Dalian University of Technology, Dalian 116024, China; University of Chinese Academy of Sciences, Beijing 100049, China.
authors.forename: Xiaobing
authors.initials: X
authors.lastname: Yang
authors.affiliation: Division of Biotechnology and Dalian National Laboratory for Clean Energy, Dalian Institute of Chemical Physics, CAS, Dalian 116023, China.
authors.forename: Guojie
authors.initials: G
authors.lastname: Jin
authors.affiliation: Division of Biotechnology and Dalian National Laboratory for Clean Energy, Dalian Institute of Chemical Physics, CAS, Dalian 116023, China.
authors.forename: Zhiwei
authors.initials: Z
authors.lastname: Gong
authors.affiliation: Division of Biotechnology and Dalian National Laboratory for Clean Energy, Dalian Institute of Chemical Physics, CAS, Dalian 116023, China.
authors.forename: Hongwei
authors.initials: H
authors.lastname: Shen
authors.affiliation: School of Life Science and Biotechnology, Dalian University of Technology, Dalian 116024, China.
authors.forename: Fengwu
authors.initials: F
authors.lastname: Bai
authors.affiliation: Division of Biotechnology and Dalian National Laboratory for Clean Energy, Dalian Institute of Chemical Physics, CAS, Dalian 116023, China. Electronic address: zhaozb@dicp.ac.cn.
authors.forename: Zongbao Kent
authors.initials: ZK
authors.lastname: Zhao
mesh_terms: D001487:Basidiomycota / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D008055:Lipids / Q000096:biosynthesis*; D059027:Recycling; D014866:Waste Products; D062065:Wastewater; D015003:Yeasts / Q000378:metabolism*
chemical_list: D005947:Glucose; D008055:Lipids; D014866:Waste Products; D062065:Wastewater
keywords: Microbial lipids; Nutrient recycling; Oleaginous yeast; Rhodosporidium toruloides; Spent cell mass