title: Increased detection of G3P[9] and G6P[9] rotavirus strains in hospitalized children with acute diarrhea in Bulgaria.
abstract: Rotavirus severe disease in children is now vaccine-preventable and the roll-out of vaccination programs globally is expected to make a significant impact in the reduction of morbidity and mortality in children <5 years of age. Rotavirus is also a pathogen of other mammals and birds, and its segmented RNA genome can lead to the emergence of new or unusual strains in human population via interspecies transmission and reassortment events. Despite the efficacy and impact of rotavirus vaccine in preventing severe diarrhea, the correlates of protection remain largely unknown. Therefore, rotavirus strain surveillance before, during and after the introduction of immunization programs remains a crucial for monitoring rotavirus vaccine efficacy and impact. In this context, molecular characterization of 1323 Bulgarian rotavirus strains collected between June 2010 and May 2013 was performed. A total of 17 strains of interest were analyzed by partial sequence analysis. Twelve strains were characterized as G3P[9] and G6P[9] of potential animal origin. Phylogenetic analysis and comparisons with the same specificity strains detected sporadically between 2006 and 2010 revealed the constant circulation of these unusual human strains in Bulgaria, although in low prevalence, and their increased potential for person-to-person spread.
authors.affiliation: National Center for Infectious and Parasitic Diseases, Sofia, Bulgaria; Public Health of England, London, United Kingdom. Electronic address: zornitsavmbg@yahoo.com.
authors.forename: Zornitsa
authors.initials: Z
authors.lastname: Mladenova
authors.affiliation: Public Health of England, London, United Kingdom.
authors.forename: Sameena
authors.initials: S
authors.lastname: Nawaz
authors.affiliation: National Institute of Cholera and Enteric Diseases, Kolkata, India.
authors.forename: Balasubramanian
authors.initials: B
authors.lastname: Ganesh
authors.affiliation: Institute of Infection and Global Health, University of Liverpool, Liverpool, United Kingdom.
authors.forename: Miren
authors.initials: M
authors.lastname: Iturriza-Gomara
mesh_terms: D002031:Bulgaria; D002675:Child, Preschool; D003968:Diarrhea, Infantile / Q000821:virology*; D005260:Female; D006760:Hospitalization; D006801:Humans; D007223:Infant; D008297:Male; D008969:Molecular Sequence Data; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics*; D012401:Rotavirus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D012400:Rotavirus Infections / Q000821:virology*; D017423:Sequence Analysis, RNA
chemical_list: D012367:RNA, Viral
keywords: Human strain; Reassortant; Rotavirus; Rotavirus vaccine; Zoonotic transmission