title: A novel serum microRNA panel to discriminate benign from malignant ovarian disease.
abstract: Ovarian cancer is the seventh most common cancer in women and the most frequent cause of gynaecological malignancy-related mortality in women. Currently, no standardized reliable screening test exists. MicroRNA profiling has allowed the identification of signatures associated with diagnosis, prognosis and response to treatment of human tumours. The aim of this study was to determine if a microRNA signature could distinguish between malignant and benign ovarian disease. A training set of 5 serous ovarian carcinomas and 5 benign serous cystadenomas were selected for the initial experiments. The validation set included 20 serous ovarian carcinomas and 20 benign serous cystadenomas. The serum/plasma focus microRNA Exiqon panel was used for the training set. For the validation set a pick and mix Exiqon panel, which focuses on microRNAs of interest was used. A panel of 4 microRNAs (let-7i-5p, miR-122, miR-152-5p and miR-25-3p) was significantly down regulated in cancer patients. These microRNAs target WNT signalling, AKT/mTOR and TLR-4/MyD88, which have previously been found to play a role in ovarian carcinogenesis and chemoresistance. let-7i-5p, miR-122, miR-152-5p and miR-25-3p could act as diagnostic biomarkers in ovarian cancer.
authors.affiliation: Department of Obstetrics and Gynaecology, Trinity College Dublin, St. James's Hospital, Dublin 8, Ireland; Department of Histopathology, Trinity College Dublin, St. James's Hospital, Dublin 8, Ireland. Electronic address: reamlanghe@yahoo.co.uk.
authors.forename: Ream
authors.initials: R
authors.lastname: Langhe
authors.affiliation: Department of Obstetrics and Gynaecology, Trinity College Dublin, St. James's Hospital, Dublin 8, Ireland.
authors.forename: Lucy
authors.initials: L
authors.lastname: Norris
authors.affiliation: Department of Gynaecological-Oncology, St. James's Hospital, Dublin 8, Ireland.
authors.forename: Feras Abu
authors.initials: FA
authors.lastname: Saadeh
authors.affiliation: Department of Histopathology, Trinity College Dublin, St. James's Hospital, Dublin 8, Ireland.
authors.forename: Gordon
authors.initials: G
authors.lastname: Blackshields
authors.affiliation: Department of Obstetrics and Gynaecology, Trinity College Dublin, St. James's Hospital, Dublin 8, Ireland.
authors.forename: Rachel
authors.initials: R
authors.lastname: Varley
authors.affiliation: Department of Obstetrics and Gynaecology, Trinity College Dublin, St. James's Hospital, Dublin 8, Ireland.
authors.forename: Ashling
authors.initials: A
authors.lastname: Harrison
authors.affiliation: Department of Gynaecological-Oncology, St. James's Hospital, Dublin 8, Ireland.
authors.forename: Noreen
authors.initials: N
authors.lastname: Gleeson
authors.affiliation: Department of Histopathology, Trinity College Dublin, Coombe Women's and Infants University Hospital, Dublin 8, Ireland.
authors.forename: Cathy
authors.initials: C
authors.lastname: Spillane
authors.affiliation: Department of Histopathology, Trinity College Dublin, Coombe Women's and Infants University Hospital, Dublin 8, Ireland.
authors.forename: Cara
authors.initials: C
authors.lastname: Martin
authors.affiliation: Department of Medical Oncology, St. James's Hospital, Dublin 8, Ireland.
authors.forename: Dearbhaile M
authors.initials: DM
authors.lastname: O'Donnell
authors.affiliation: Department of Gynaecological-Oncology, St. James's Hospital, Dublin 8, Ireland.
authors.forename: Tom
authors.initials: T
authors.lastname: D'Arcy
authors.affiliation: Department of Histopathology, Trinity College Dublin, St. James's Hospital, Dublin 8, Ireland; Department of Histopathology, Trinity College Dublin, Coombe Women's and Infants University Hospital, Dublin 8, Ireland.
authors.forename: John
authors.initials: J
authors.lastname: O'Leary
authors.affiliation: Department of Obstetrics and Gynaecology, Trinity College Dublin, St. James's Hospital, Dublin 8, Ireland; Department of Histopathology, Trinity College Dublin, St. James's Hospital, Dublin 8, Ireland.
authors.forename: Sharon
authors.initials: S
authors.lastname: O'Toole
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000097:blood* / Q000235:genetics; D016022:Case-Control Studies; D018284:Cystadenocarcinoma, Serous / Q000097:blood / Q000175:diagnosis* / Q000235:genetics; D005260:Female; D005500:Follow-Up Studies; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006461:Hemolysis; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000097:blood* / Q000235:genetics*; D008875:Middle Aged; D009367:Neoplasm Staging; D010051:Ovarian Neoplasms / Q000097:blood / Q000175:diagnosis* / Q000235:genetics; D011379:Prognosis; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D014408:Biomarkers, Tumor; D035683:MicroRNAs; D012333:RNA, Messenger
keywords: MicroRNAs; Serous ovarian cancer; Serum; panel