title: Combining urban wastewater treatment with biohydrogen production--an integrated microalgae-based approach.
abstract: The aim of the present work was the simultaneous treatment of urban wastewater using microalgae and the energetic valorization of the obtained biomass. Chlorella vulgaris (Cv), Scenedesmus obliquus (Sc) and a naturally occurring algal Consortium C (ConsC) were grown in an urban wastewater. The nutrient removals were quite high and the treated water fits the legislation (PT Dec-Lei 236/98) in what concerns the parameters analysed (N, P, COD). After nutrient depletion the microalgae remained two more weeks in the photobioreactor (PBR) under nutritional stress conditions, to induce sugar accumulation (22-43%). The stressed biomass was converted into biohydrogen (bioH2), a clean energy carrier, through dark fermentation by a strain of the bacteria Enterobacter aerogenes. The fermentation kinetics were monitored and fitted to a modified Gompertz model. The highest bioH2 production yield was obtained for S. obliquus (56.8 mL H2/gVS) which was very similar when using the same algae grown in synthetic media.
authors.affiliation: LNEG-Laboratório Nacional de Engenharia e Geologia, I.P. Unidade de Bioenergia, Estrada do Paço do Lumiar 22, 1649-038 Lisboa, Portugal.
authors.forename: Ana Paula
authors.initials: AP
authors.lastname: Batista
authors.affiliation: Universidade Federal de Lavras, Programa de Pós-Graduação Fitotecnia/Agronomia, Lavras, Brazil.
authors.forename: Lucas
authors.initials: L
authors.lastname: Ambrosano
authors.affiliation: LNEG-Laboratório Nacional de Engenharia e Geologia, I.P. Unidade de Bioenergia, Estrada do Paço do Lumiar 22, 1649-038 Lisboa, Portugal.
authors.forename: Sofia
authors.initials: S
authors.lastname: Graça
authors.affiliation: LNEG-Laboratório Nacional de Engenharia e Geologia, I.P. Unidade de Bioenergia, Estrada do Paço do Lumiar 22, 1649-038 Lisboa, Portugal.
authors.forename: Catarina
authors.initials: C
authors.lastname: Sousa
authors.affiliation: LNEG-Laboratório Nacional de Engenharia e Geologia, I.P. Unidade de Bioenergia, Estrada do Paço do Lumiar 22, 1649-038 Lisboa, Portugal.
authors.forename: Paula A S S
authors.initials: PASS
authors.lastname: Marques
authors.affiliation: LNEG-Laboratório Nacional de Engenharia e Geologia, I.P. Unidade de Bioenergia, Estrada do Paço do Lumiar 22, 1649-038 Lisboa, Portugal.
authors.forename: Belina
authors.initials: B
authors.lastname: Ribeiro
authors.affiliation: Universidade Federal de Lavras, Programa de Pós-Graduação Fitotecnia/Agronomia, Lavras, Brazil.
authors.forename: Elberis P
authors.initials: EP
authors.lastname: Botrel
authors.affiliation: Universidade Federal de Lavras, Programa de Pós-Graduação Fitotecnia/Agronomia, Lavras, Brazil.
authors.forename: Pedro
authors.initials: P
authors.lastname: Castro Neto
authors.affiliation: LNEG-Laboratório Nacional de Engenharia e Geologia, I.P. Unidade de Bioenergia, Estrada do Paço do Lumiar 22, 1649-038 Lisboa, Portugal. Electronic address: luisa.gouveia@lneg.pt.
authors.forename: Luisa
authors.initials: L
authors.lastname: Gouveia
mesh_terms: D056804:Biofuels / Q000382:microbiology*; D018533:Biomass; D001709:Biotechnology / Q000379:methods*; D002708:Chlorella / Q000254:growth & development; D002947:Cities; D005285:Fermentation; D006859:Hydrogen / Q000378:metabolism*; D007700:Kinetics; D019344:Lactic Acid / Q000378:metabolism; D058086:Microalgae / Q000254:growth & development / Q000378:metabolism*; D058433:Photobioreactors / Q000382:microbiology; D011174:Portugal; D011422:Propionates / Q000378:metabolism; D044444:Scenedesmus / Q000254:growth & development; D019802:Succinic Acid / Q000378:metabolism; D062065:Wastewater / Q000382:microbiology; D018508:Water Purification
chemical_list: D056804:Biofuels; D006859:Hydrogen; D019344:Lactic Acid; D011422:Propionates; D019802:Succinic Acid; D062065:Wastewater; C029658:propionic acid
keywords: Biohydrogen; Dark fermentation; Microalgae; Wastewater treatment