title: Rapid and sensitive HPLC-MS/MS method for quantitative determination of lycorine from the plasma of rats.
abstract: A simple, rapid and sensitive high-performance liquid chromatography-tandem mass spectrometric (HPLC-MS/MS) method was developed and validated for determining lycorine in rat plasma. Plasma samples were prepared by a simple protein precipitation with methanol containing dextrorphan as internal standard. The chromatographic separation was performed on a Kromasil 60-5CN column (3 μm, 2.1 mm × 150 mm) with the mobile phase of methanol/water (containing 0.1% formic acid) (40:60, v/v) at a flow rate of 0.2 mL/min, the total analytical runtime was 5 min. The detection was performed on a triple quadrupole tandem mass spectrometer equipped with Electronic Spray Ion by selected reaction monitoring (SRM) of the transitions at m/z 288.1→147.1 for lycorine and m/z 258.1→157.2 for dextrorphan, respectively. The calibration curve was linear over the range of 1-1000 ng/mL with the lower limit of quantification of 1 ng/mL for lycorine. The intra- and inter-day precision (R.S.D.%) were less than 8.5% and accuracy (R.E.%) was within ±7.0%. Lycorine was sufficiently stable under all relevant analytical conditions. This method was successfully applied to the pharmacokinetic study of lycorine in rats after intraperitoneal administration with different doses of 5, 10 and 20 mg/kg.
authors.affiliation: Department of Drug Metabolism, Key Laboratory of Active Substances Discovery and Drug Ability Evaluation, State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, PR China.
authors.forename: Xin
authors.initials: X
authors.lastname: Liu
authors.affiliation: Department of Pharmacy, Wuxi People's Hospital, Nanjing Medical University, Wuxi 214023, PR China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Hong
authors.affiliation: Institute of Drug Clinical Trial, Wuxi People's Hospital, Nanjing Medical University, Wuxi 214023, PR China.
authors.forename: Qing
authors.initials: Q
authors.lastname: He
authors.affiliation: Institute of Drug Clinical Trial, Wuxi People's Hospital, Nanjing Medical University, Wuxi 214023, PR China. Electronic address: hk19820627@sina.com.
authors.forename: Kai
authors.initials: K
authors.lastname: Huang
mesh_terms: D047151:Amaryllidaceae Alkaloids / Q000097:blood* / Q000493:pharmacokinetics; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D057230:Limit of Detection; D008297:Male; D010617:Phenanthridines / Q000097:blood* / Q000493:pharmacokinetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D047151:Amaryllidaceae Alkaloids; D010617:Phenanthridines; C015330:lycorine
keywords: HPLC–MS/MS; Lycorine; Pharmacokinetics; Plasma concentration