title: An R2R3-MYB gene, LeAN2, positively regulated the thermo-tolerance in transgenic tomato.
abstract: LeAN2 is an anthocyanin-associated R2R3-MYB transcription factor, but little is known about its function in imparting thermo-tolerance to higher plants. To examine the function of LeAN2 in the regulation of heat stress in tomato, LeAN2 was isolated and transgenic tomato plants were obtained. Overexpression of LeAN2 under the control of the CaMV35S promoter in tomato induced the up-regulation of several structural genes in the anthocyanin biosynthetic pathway as well as anthocyanin accumulation in transgenic tomato plants. Transgenic tomato plants showed enhanced tolerance to heat stress by maintaining higher fresh weight (FW), net photosynthetic rate (Pn) and maximal photochemical efficiency of photosystem II (PSII) (Fv/Fm) compared with wild-type (WT) plants. Furthermore, transgenic plants showed higher non-enzymatic antioxidant activity, lower levels of reactive oxygen species (ROS), and higher contents of D1 protein than that in WT plants under heat stress. These results indicate that LeAN2 had an important function in heat stress resistance.
authors.affiliation: College of Life Science, State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai'an, Shandong 271018, PR China.
authors.forename: Xia
authors.initials: X
authors.lastname: Meng
authors.affiliation: College of Life Science, State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai'an, Shandong 271018, PR China.
authors.forename: Jie-Ru
authors.initials: JR
authors.lastname: Wang
authors.affiliation: College of Life Science, State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai'an, Shandong 271018, PR China.
authors.forename: Guo-Dong
authors.initials: GD
authors.lastname: Wang
authors.affiliation: College of Life Science, State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai'an, Shandong 271018, PR China.
authors.forename: Xiao-Qing
authors.initials: XQ
authors.lastname: Liang
authors.affiliation: College of Life Science, State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai'an, Shandong 271018, PR China.
authors.forename: Xiao-Dong
authors.initials: XD
authors.lastname: Li
authors.affiliation: College of Life Science, State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai'an, Shandong 271018, PR China. Electronic address: qwmeng@sdau.edu.cn.
authors.forename: Qing-Wei
authors.initials: QW
authors.lastname: Meng
mesh_terms: D000872:Anthocyanins / Q000378:metabolism*; D000975:Antioxidants / Q000378:metabolism; D015870:Gene Expression; D018506:Gene Expression Regulation, Plant; D006358:Hot Temperature; D018551:Solanum lycopersicum / Q000235:genetics* / Q000502:physiology; D010788:Photosynthesis / Q000502:physiology; D045332:Photosystem II Protein Complex / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D030821:Plants, Genetically Modified; D017382:Reactive Oxygen Species / Q000378:metabolism; D013312:Stress, Physiological; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism
chemical_list: D000872:Anthocyanins; D000975:Antioxidants; D045332:Photosystem II Protein Complex; D010940:Plant Proteins; D017382:Reactive Oxygen Species; D014157:Transcription Factors
keywords: Anthocyanin; Heat stress; LeAN2; Tomato; Transgenic plants