title: Roseoloviruses manipulate host cell cycle.
abstract: During lytic infections HHV-6A and HHV-6B disrupt E2F1-Rb complexes by Rb degradation, releasing E2F1 and driving the infected cells toward the S-phase. Whereas upon infection E2F1 and its cofactor DP1 were up-regulated, additional E2F responsive genes were expressed differentially in various cells. E2F binding sites were identified in promoters of several HHV-6 genes, including the U27 and U79 associated with viral DNA replication, revealing high dependence on the binding site and the effect of the E2F1 transcription factor. Viral genes regulation by E2F1 can synchronize viral replication with the optimal cell cycle phase, enabling utilization of host resources for successful viral replication. Furthermore, it was found that infection by roseoloviruses leads to cell cycle arrest, mostly in the G2/M-phase.
authors.affiliation: Department of Cell Research and Immunology and the S. Daniel Abraham Institute for Molecular Virology, Tel Aviv University, Tel Aviv 69978, Israel. Electronic address: nfrenkel@post.tau.ac.il.
authors.forename: Niza
authors.initials: N
authors.lastname: Frenkel
authors.affiliation: Department of Cell Research and Immunology and the S. Daniel Abraham Institute for Molecular Virology, Tel Aviv University, Tel Aviv 69978, Israel.
authors.forename: Eyal
authors.initials: E
authors.lastname: Sharon
authors.affiliation: Department of Cell Research and Immunology and the S. Daniel Abraham Institute for Molecular Virology, Tel Aviv University, Tel Aviv 69978, Israel.
authors.forename: Haim
authors.initials: H
authors.lastname: Zeigerman
mesh_terms: D001665:Binding Sites; D002453:Cell Cycle; D050687:E2F1 Transcription Factor / Q000378:metabolism; D015967:Gene Expression Regulation, Viral; D054884:Host-Pathogen Interactions; D011401:Promoter Regions, Genetic; D016160:Retinoblastoma Protein / Q000378:metabolism; D019211:Roseolovirus / Q000502:physiology*; D050680:Transcription Factor DP1 / Q000378:metabolism; D014764:Viral Proteins / Q000096:biosynthesis / Q000235:genetics; D014779:Virus Replication
chemical_list: D050687:E2F1 Transcription Factor; C493753:E2F1 protein, human; D016160:Retinoblastoma Protein; D050680:Transcription Factor DP1; D014764:Viral Proteins