title: A novel composite of molecularly imprinted polymer-coated PdNPs for electrochemical sensing norepinephrine.
abstract: A novel composite of molecularly imprinted polymer-coated palladium nanoparticles (MIP-coated PdNPs) was synthesized by sol-gel method using norepinephrine as template, phenyl trimethoxysilane as functional monomer and tetramethoxysilane as crosslinker. The combination of PdNPs and silica-based MIP endowed the composite with good electrochemical catalytic property, large surface area and template selectivity. MIP-coated PdNPs were characterized by Fourier transform infrared spectroscopy and Transmission electron microscopy. Then MIP-coated PdNPs composite was used as a recognition element in the construction of an electrochemical sensor for norepinephrine. The properties of MIP-coated PdNPs sensor such as special binding, adsorption dynamics and selective recognition ability were evaluated by differential pulse voltammetry. The results demonstrated that MIP-coated PdNPs sensor not only possessed a short response time, but also high binding capacity for norepinephrine, which enabled the imprinted sensor with higher current response than that of non-imprinted material and MIP without PdNPs. In addition, the MIP-coated PdNPs sensor exhibited selectivity for norepinephrine in comparison to other analogs. The MIP-coated PdNPs sensor had a wide linear range over norepinephrine concentration from 0.5 to 80.0μM with a detection limit of 0.1μM. The MIP-coated PdNPs sensor was proved to be a suitable sensing tool for the fast, sensitive and selective determination of norepinephrine in injection and urine samples.
authors.affiliation: College of Chemistry and Life Science, Zhejiang Normal University, Jinhua 321004, PR China.
authors.forename: Jianrong
authors.initials: J
authors.lastname: Chen
authors.affiliation: College of Chemistry and Life Science, Zhejiang Normal University, Jinhua 321004, PR China; College of Biological, Chemical Sciences and Engineering, Jiaxing University, Jiaxing 314001, PR China.
authors.forename: Hong
authors.initials: H
authors.lastname: Huang
authors.affiliation: College of Biological, Chemical Sciences and Engineering, Jiaxing University, Jiaxing 314001, PR China. Electronic address: ybzeng@mail.zjxu.edu.cn.
authors.forename: Yanbo
authors.initials: Y
authors.lastname: Zeng
authors.affiliation: College of Chemistry and Life Science, Zhejiang Normal University, Jinhua 321004, PR China; College of Biological, Chemical Sciences and Engineering, Jiaxing University, Jiaxing 314001, PR China.
authors.forename: Huan
authors.initials: H
authors.lastname: Tang
authors.affiliation: College of Chemistry and Life Science, Zhejiang Normal University, Jinhua 321004, PR China; College of Biological, Chemical Sciences and Engineering, Jiaxing University, Jiaxing 314001, PR China. Electronic address: lei.li@mail.zjxu.edu.cn.
authors.forename: Lei
authors.initials: L
authors.lastname: Li
mesh_terms: D000328:Adult; D015374:Biosensing Techniques / Q000379:methods; D055664:Electrochemical Techniques / Q000379:methods*; D006801:Humans; D057230:Limit of Detection; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D054802:Molecular Imprinting / Q000379:methods*; D009638:Norepinephrine / Q000032:analysis / Q000652:urine*; D010165:Palladium / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*
chemical_list: D011108:Polymers; D010165:Palladium; D009638:Norepinephrine
keywords: Electrochemical sensor; Molecularly imprinted polymer; Norepinephrine; Palladium nanoparticles