title: Differential expression of immune-related cytokine genes in response to J group avian leukosis virus infection in vivo.
abstract: Infection with J group avian leukosis virus (ALV-J) can result in immunosuppression and subsequently increased susceptibility to secondary infection. The innate immune system is the first line defense system in prevention of further bacterial and viral infections. Cytokines play key roles in the innate immune system. In this study, we used RT-qPCR technology to test the cytokine mRNA expression levels in various immune tissues, including the spleen, bursa of fabricius and cecal tonsil, in the days following ALV-J infection. The results indicated that in the infected group, the expression levels of interleukin-6 (IL-6), IL-18, interferon-α (IFN-α) and IFN-γ significantly increased in the spleen and reached peak levels that were thousandfolds higher than baselines at 9-12 days post-infection (d.p.i.). The levels in the bursa of fabricius slightly increased, and the levels in the cecal tonsil were not significantly altered. Moreover, the pattern of the expression of these three cytokines in the spleens of the infected group was similar to the pattern of viremia of this group. These results suggest that the spleen plays an important role in the interaction between ALV-J infection and the innate immune system. This study contributes to the understanding of innate immune responses to ALV-J infection and also elucidates the mechanisms of the pathogenicity of ALV-J in chickens.
authors.affiliation: Division of Avian Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, No. 427, Maduan Street, Nan Gang District, Harbin 150001, Heilongjiang Province, PR China. Electronic address: yngao03@sina.com.
authors.forename: Yanni
authors.initials: Y
authors.lastname: Gao
authors.affiliation: Division of Avian Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, No. 427, Maduan Street, Nan Gang District, Harbin 150001, Heilongjiang Province, PR China. Electronic address: 651854815@qq.com.
authors.forename: Yongzhen
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Division of Avian Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, No. 427, Maduan Street, Nan Gang District, Harbin 150001, Heilongjiang Province, PR China. Electronic address: guanxiaolu07@163.com.
authors.forename: Xiaolu
authors.initials: X
authors.lastname: Guan
authors.affiliation: Division of Avian Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, No. 427, Maduan Street, Nan Gang District, Harbin 150001, Heilongjiang Province, PR China. Electronic address: 965232411@qq.com.
authors.forename: Xiaofei
authors.initials: X
authors.lastname: Li
authors.affiliation: Division of Avian Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, No. 427, Maduan Street, Nan Gang District, Harbin 150001, Heilongjiang Province, PR China. Electronic address: yunbingling2010@126.com.
authors.forename: Bingling
authors.initials: B
authors.lastname: Yun
authors.affiliation: Division of Avian Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, No. 427, Maduan Street, Nan Gang District, Harbin 150001, Heilongjiang Province, PR China. Electronic address: qxl@hvri.ac.cn.
authors.forename: Xiaole
authors.initials: X
authors.lastname: Qi
authors.affiliation: Division of Avian Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, No. 427, Maduan Street, Nan Gang District, Harbin 150001, Heilongjiang Province, PR China. Electronic address: yqw@hvri.ac.cn.
authors.forename: Yongqiang
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Division of Avian Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, No. 427, Maduan Street, Nan Gang District, Harbin 150001, Heilongjiang Province, PR China. Electronic address: ghl@hvri.ac.cn.
authors.forename: Honglei
authors.initials: H
authors.lastname: Gao
authors.affiliation: Division of Avian Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, No. 427, Maduan Street, Nan Gang District, Harbin 150001, Heilongjiang Province, PR China. Electronic address: cuihongyu@caas.cn.
authors.forename: Hongyu
authors.initials: H
authors.lastname: Cui
authors.affiliation: Division of Avian Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, No. 427, Maduan Street, Nan Gang District, Harbin 150001, Heilongjiang Province, PR China. Electronic address: liucj93711@hvri.ac.cn.
authors.forename: Changjun
authors.initials: C
authors.lastname: Liu
authors.affiliation: Division of Avian Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, No. 427, Maduan Street, Nan Gang District, Harbin 150001, Heilongjiang Province, PR China. Electronic address: zhangyanping03@caas.cn.
authors.forename: Yanping
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Division of Avian Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, No. 427, Maduan Street, Nan Gang District, Harbin 150001, Heilongjiang Province, PR China; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Disease and Zoonoses, Yangzhou 225009, PR China. Electronic address: xmw@hvri.ac.cn.
authors.forename: Xiaomei
authors.initials: X
authors.lastname: Wang
authors.affiliation: Division of Avian Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, No. 427, Maduan Street, Nan Gang District, Harbin 150001, Heilongjiang Province, PR China. Electronic address: ylg@hvri.ac.cn.
authors.forename: Yulong
authors.initials: Y
authors.lastname: Gao
mesh_terms: D000818:Animals; D001353:Avian Leukosis / Q000235:genetics* / Q000276:immunology / Q000821:virology*; D001354:Avian Leukosis Virus / Q000502:physiology*; D002060:Bursa of Fabricius / Q000378:metabolism / Q000473:pathology / Q000821:virology; D002432:Cecum / Q000378:metabolism / Q000473:pathology / Q000821:virology; D002645:Chickens / Q000235:genetics / Q000276:immunology* / Q000821:virology; D016207:Cytokines / Q000235:genetics* / Q000378:metabolism; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D007109:Immunity / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012015:Reference Standards; D013154:Spleen / Q000378:metabolism / Q000821:virology; D013997:Time Factors; D014766:Viremia / Q000235:genetics / Q000821:virology
chemical_list: D016207:Cytokines; D012333:RNA, Messenger
keywords: ALV-J; Innate immune responses; Interferon; Interleukin