title: Sex-dependent transcriptome analysis and single nucleotide polymorphism (SNP) discovery in the brine shrimp Artemia franciscana.
abstract: In order to enhance genomic resources for the brine shrimp Artemia franciscana, RNA-Seq analysis was conducted for adult females and males. Through de novo assembly, 36,896 high quality contigs were obtained, of which 13,749 sequences were annotated with arthropod sequences. Just 4.5% matched against previously reported sequences for Artemia spp. Additionally, different transcriptional patterns between males and females were found, evidencing sex-related transcriptional responses. Furthermore, 221 and 534 putative SNPs were identified exclusively in males and females, respectively. These results will build the foundation for further genomic studies in A. franciscana.
authors.affiliation: Laboratory of Biotechnology and Aquatic Genomics, Interdisciplinary Center for Aquaculture Research (INCAR), University of Concepción, P.O. Box 160-C, Chile.
authors.forename: Diego
authors.initials: D
authors.lastname: Valenzuela-Miranda
authors.affiliation: Laboratory of Biotechnology and Aquatic Genomics, Interdisciplinary Center for Aquaculture Research (INCAR), University of Concepción, P.O. Box 160-C, Chile. Electronic address: crisgallardo@udec.cl.
authors.forename: Cristian
authors.initials: C
authors.lastname: Gallardo-Escárate
authors.affiliation: Laboratory of Biotechnology and Aquatic Genomics, Interdisciplinary Center for Aquaculture Research (INCAR), University of Concepción, P.O. Box 160-C, Chile.
authors.forename: Valentina
authors.initials: V
authors.lastname: Valenzuela-Muñoz
authors.affiliation: Laboratory of Biotechnology and Aquatic Genomics, Interdisciplinary Center for Aquaculture Research (INCAR), University of Concepción, P.O. Box 160-C, Chile.
authors.forename: Rodolfo
authors.initials: R
authors.lastname: Farlora
authors.affiliation: Laboratorio de Genética, Acuicultura & Biodiversidad, Universidad de Los Lagos, Avda. Fuchslocher 1305 Osorno, Chile. Electronic address: ggajardo@ulagos.cl.
authors.forename: Gonzalo
authors.initials: G
authors.lastname: Gajardo
keywords: Artemia; Brine shrimp; RNA-Seq; SNP; Transcriptome