title: Effects of carbon dioxide on cell growth and propionic acid production from glycerol and glucose by Propionibacterium acidipropionici.
abstract: The effects of CO2 on propionic acid production and cell growth in glycerol or glucose fermentation were investigated in this study. In glycerol fermentation, the volumetric productivity of propionic acid with CO2 supplementation reached 2.94g/L/day, compared to 1.56g/L/day without CO2. The cell growth using glycerol was also significantly enhanced with CO2. In addition, the yield and productivity of succinate, the main intermediate in Wood-Werkman cycle, increased 81% and 280%, respectively; consistent with the increased activities of pyruvate carboxylase and propionyl CoA transferase, two key enzymes in the Wood-Werkman cycle. However, in glucose fermentation CO2 had minimal effect on propionic acid production and cell growth. The carbon flux distributions using glycerol or glucose were also analyzed using a stoichiometric metabolic model. The calculated maintenance coefficient (mATP) increased 100%, which may explain the increase in the productivity of propionic acid in glycerol fermentation with CO2 supplement.
authors.affiliation: William G. Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University, 140 West 19th Avenue, Columbus, OH 43210, USA.
authors.forename: An
authors.initials: A
authors.lastname: Zhang
authors.affiliation: William G. Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University, 140 West 19th Avenue, Columbus, OH 43210, USA.
authors.forename: Jianxin
authors.initials: J
authors.lastname: Sun
authors.affiliation: William G. Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University, 140 West 19th Avenue, Columbus, OH 43210, USA.
authors.forename: Zhongqiang
authors.initials: Z
authors.lastname: Wang
authors.affiliation: William G. Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University, 140 West 19th Avenue, Columbus, OH 43210, USA.
authors.forename: Shang-Tian
authors.initials: ST
authors.lastname: Yang
authors.affiliation: Elixir Bioscience LLC, 1119 Jewel Creek Dr., Cary, NC 27519, USA. Electronic address: haiying.zhou09@gmail.com.
authors.forename: Haiying
authors.initials: H
authors.lastname: Zhou
mesh_terms: D002244:Carbon / Q000494:pharmacology; D002245:Carbon Dioxide / Q000494:pharmacology*; D015256:Coenzyme A-Transferases / Q000378:metabolism; D005285:Fermentation / Q000187:drug effects; D005947:Glucose / Q000378:metabolism*; D005990:Glycerol / Q000378:metabolism*; D007700:Kinetics; D053858:Metabolic Networks and Pathways / Q000187:drug effects; D008962:Models, Theoretical; D011422:Propionates / Q000378:metabolism*; D011424:Propionibacterium / Q000187:drug effects / Q000201:enzymology / Q000254:growth & development* / Q000378:metabolism*; D011766:Pyruvate Carboxylase / Q000378:metabolism
chemical_list: D011422:Propionates; D002245:Carbon Dioxide; D002244:Carbon; D015256:Coenzyme A-Transferases; D011766:Pyruvate Carboxylase; D005947:Glucose; C029658:propionic acid; D005990:Glycerol
keywords: Carbon dioxide; Glycerol; Propionibacterium acidipropionici; Propionic acid