title: Production of validamycin A from hemicellulose hydrolysate by Streptomyces hygroscopicus 5008.
abstract: Validamycin A (VAL-A) is an important agricultural antibiotic produced by Streptomyces hygroscopicus 5008, which uses starch as carbon source occupying about 20% of total production cost. To reduce the medium cost, corncob hydrolysate - a hemicellulose hydrolysate was applied as a low-cost substrate to VAL-A fermentation. It was found that three major sugars in corncob hydrolysate including d-glucose, d-xylose and l-arabinose could all be utilized by S. hygroscopicus 5008 to produce VAL-A while d-xylose was the main contributor. A higher VAL-A production titer from d-xylose was achieved by using a genetically engineered strain TC03 derived from S. hygroscopicus 5008, which resulted in 1.27-fold improvement of VAL-A production from the medium containing 13% (v/v) corncob hydrolysate compared to that by its original strain. A medium cost analysis was done and compared with previous reports. This work indicates a great potential of the hemicellulose hydrolysate as substrate for antibiotic fermentation.
authors.affiliation: State Key Laboratory of Microbial Metabolism, and Laboratory of Molecular Biochemical Engineering, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, 800 Dong-Chuan Road, Shanghai 200240, China.
authors.forename: Tan-Che
authors.initials: TC
authors.lastname: Zhou
authors.affiliation: State Key Laboratory of Microbial Metabolism, and Laboratory of Molecular Biochemical Engineering, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, 800 Dong-Chuan Road, Shanghai 200240, China; Shanghai Collaborative Innovation Center for Biomanufacturing Technology (SCICBT), East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, China. Electronic address: jjzhong@sjtu.edu.cn.
authors.forename: Jian-Jiang
authors.initials: JJ
authors.lastname: Zhong
mesh_terms: D001089:Arabinose / Q000378:metabolism; D002244:Carbon / Q000378:metabolism; D005285:Fermentation; D005947:Glucose / Q000378:metabolism; D006868:Hydrolysis; D007218:Industrial Microbiology / Q000379:methods*; D007294:Inositol / Q000031:analogs & derivatives* / Q000096:biosynthesis; D011134:Polysaccharides / Q000378:metabolism*; D013302:Streptomyces / Q000235:genetics / Q000378:metabolism*; D014994:Xylose / Q000378:metabolism; D003313:Zea mays / Q000737:chemistry
chemical_list: D011134:Polysaccharides; C030790:validamycin A; D007294:Inositol; D002244:Carbon; C007916:hemicellulose; D014994:Xylose; D001089:Arabinose; D005947:Glucose
keywords: Antibiotics fermentation; Hemicellulose hydrolysate; Streptomyces hygroscopicus; Validamycin A; d-Xylose