title: Effect of poly(amidoamine) dendrimers on the structure and activity of immune molecules.
abstract: BACKGROUND
  Poly(amidoamine) (PAMAM) dendrimers are widely used biomedical polymers, which are extensively applied in drug delivery, gene delivery, contrast agent, etc. In these biomedical applications, the bio-safety of the PAMAM dendrimers is a critical issue, which affects not only their toxicity to the host but also the expected in vivo biofunctions of the materials. To clarify the bio-safety of PAMAM dendrimers, the effects of generation 5 PAMAM dendrimers with amine, hydroxyl or carboxyl groups on immune molecules were explored in this work.
  
  
  METHODS
  Specifically, the effect of the PAMAM dendrimers on the secondary structure and conformation of immune molecule γ-globulin was studied by using ultraviolet-visible, fluorescence, and circular dichroism spectroscopies. The effect of the PAMAM dendrimers on complement activation was determined by enzyme-linked immunosorbent assay. Further, the effect of the PAMAM dendrimers on antigen-antibody reaction was studied by using human red blood cell agglutination assay.
  
  
  RESULTS
  The results showed that, the PAMAM dendrimers could affect the secondary structure and conformation of γ-globulin, and inhibited complement activation. Generation 5 PAMAM dendrimer with carboxyl group at 10mg/mL impaired red blood cell (RBC) antigen-antibody reaction.
  
  
  CONCLUSIONS
  From these results, the effects of the PAMAM dendrimers on immune molecules depend on their bulk structure and surface groups.
  
  
  CONCLUSIONS
  This work provides important information for the immunocompatibility evaluation, preclinical design, and clinical applications of PAMAM dendrimers.
authors.affiliation: Department of Anatomy, Key Laboratory of Construction and Detection of Guangdong Province, Southern Medical University, Guangzhou 510515, China.
authors.forename: Jiansheng
authors.initials: J
authors.lastname: Lin
authors.affiliation: Department of Anatomy, Key Laboratory of Construction and Detection of Guangdong Province, Southern Medical University, Guangzhou 510515, China.
authors.forename: Wenxi
authors.initials: W
authors.lastname: Hua
authors.affiliation: Department of Biomedical Engineering, Jinan University, Guangzhou 510632, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Biomedical Engineering, Jinan University, Guangzhou 510632, China.
authors.forename: Chenghua
authors.initials: C
authors.lastname: Li
authors.affiliation: Department of Biomedical Engineering, Jinan University, Guangzhou 510632, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Xue
authors.affiliation: Department of Anatomy, Hunan University of Chinese Medicine, Changsha 410208, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Yin
authors.affiliation: Department of Biomedical Engineering, Jinan University, Guangzhou 510632, China. Electronic address: tliuzonghua@jnu.edu.cn.
authors.forename: Zonghua
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Department of Anatomy, Key Laboratory of Construction and Detection of Guangdong Province, Southern Medical University, Guangzhou 510515, China. Electronic address: qiuxzh@aliyun.com.
authors.forename: Xiaozhong
authors.initials: X
authors.lastname: Qiu
mesh_terms: D000937:Antigen-Antibody Reactions; D050091:Dendrimers / Q000737:chemistry*; D004912:Erythrocytes / Q000737:chemistry* / Q000276:immunology; D005260:Female; D006801:Humans; D008297:Male; D013329:Structure-Activity Relationship; D005719:gamma-Globulins / Q000737:chemistry* / Q000276:immunology
chemical_list: D050091:Dendrimers; C104700:PAMAM Starburst; D005719:gamma-Globulins
keywords: Biocompatibility; Biomedical material; Immunocompatibility; Poly(amidoamine)