title: GABA and 5-HT systems are implicated in the anxiolytic-like effect of spinosin in mice.
abstract: The present study investigated the anxiolytic-like effects of spinosin, one of the major flavonoids in Ziziphi Spinosae Semen (ZSS), in experimental models of anxiety compared with a known anxiolytic, diazepam. Repeated treatment with spinosin (2.5 and 5mg/kg/day, p.o.) significantly increased the percentage of entries into and time spent on the open arms of the elevated plus maze compared with the control group. In the light/dark box test, spinosin exerted an anxiolytic-like effect at 5mg/kg. In the open-field test, 5mg/kg spinosin increased the number of central entries. Spinosin did not affect spontaneous activity. The anxiolytic-like effects of spinosin in the elevated plus maze, light/dark box test, and open field test were blocked by the γ-aminobutyric acid-A (GABAA) receptor antagonist flumazenil (3mg/kg, i.p.) and 5-hydroxytryptamine-1A (5-HT1A) receptor antagonist WAY-100635 (1mg/kg, i.p.). These results suggest that spinosin exerts anxiolytic-like effects, and its mechanism of action appears to be modulated by GABAA and 5-HT1A receptors.
authors.affiliation: School of Chinese Materia Medica, Beijing University of Chinese Medicine, 6A Wangjing Central South Road, Chaoyang District, Beijing 100102, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Liu
authors.affiliation: Standard of Chinese medicine, State Pharmacopoeia Commission, 11A Law of Southern China, Chongwen District, Beijing 100061, China.
authors.forename: Wei-Min
authors.initials: WM
authors.lastname: Zhai
authors.affiliation: School of Pharmacy, Zhejiang Chinese Medical University, 548 Binwen Road, Hangzhou 310053, PR China.
authors.forename: Yuan-Xiao
authors.initials: YX
authors.lastname: Yang
authors.affiliation: School of Chinese Materia Medica, Beijing University of Chinese Medicine, 6A Wangjing Central South Road, Chaoyang District, Beijing 100102, China. Electronic address: shijl@vip.sina.com.
authors.forename: Jin-Li
authors.initials: JL
authors.lastname: Shi
authors.affiliation: School of Chinese Materia Medica, Beijing University of Chinese Medicine, 6A Wangjing Central South Road, Chaoyang District, Beijing 100102, China.
authors.forename: Qian-Tong
authors.initials: QT
authors.lastname: Liu
authors.affiliation: School of Chinese Materia Medica, Beijing University of Chinese Medicine, 6A Wangjing Central South Road, Chaoyang District, Beijing 100102, China.
authors.forename: Guo-Lin
authors.initials: GL
authors.lastname: Liu
authors.affiliation: School of Chinese Materia Medica, Beijing University of Chinese Medicine, 6A Wangjing Central South Road, Chaoyang District, Beijing 100102, China.
authors.forename: Nan
authors.initials: N
authors.lastname: Fang
authors.affiliation: School of Chinese Materia Medica, Beijing University of Chinese Medicine, 6A Wangjing Central South Road, Chaoyang District, Beijing 100102, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Li
authors.affiliation: Key Laboratory of Mental Health, Institute of Psychology, Chinese Academy of Sciences, 4A Datun Road, Chaoyang District, Beijing 100101, China. Electronic address: guojy@psych.ac.cn.
authors.forename: Jian-You
authors.initials: JY
authors.lastname: Guo
mesh_terms: D000818:Animals; D014151:Anti-Anxiety Agents / Q000494:pharmacology*; D001007:Anxiety / Q000188:drug therapy / Q000503:physiopathology / Q000523:psychology; D001522:Behavior, Animal / Q000187:drug effects / Q000502:physiology; D004365:Drugs, Chinese Herbal / Q000494:pharmacology; D005419:Flavonoids / Q000494:pharmacology*; D005442:Flumazenil / Q000494:pharmacology; D058787:GABA-A Receptor Antagonists / Q000494:pharmacology; D008297:Male; D018782:Maze Learning / Q000187:drug effects / Q000502:physiology; D051379:Mice; D008813:Mice, Inbred ICR; D009043:Motor Activity / Q000187:drug effects / Q000502:physiology; D010879:Piperazines / Q000494:pharmacology; D011725:Pyridines / Q000494:pharmacology; D044282:Receptor, Serotonin, 5-HT1A / Q000502:physiology; D011963:Receptors, GABA-A / Q000502:physiology; D012701:Serotonin / Q000502:physiology*; D058829:Serotonin 5-HT1 Receptor Antagonists / Q000494:pharmacology; D005680:gamma-Aminobutyric Acid / Q000502:physiology*
chemical_list: D014151:Anti-Anxiety Agents; D004365:Drugs, Chinese Herbal; D005419:Flavonoids; D058787:GABA-A Receptor Antagonists; D010879:Piperazines; D011725:Pyridines; D011963:Receptors, GABA-A; D058829:Serotonin 5-HT1 Receptor Antagonists; D044282:Receptor, Serotonin, 5-HT1A; D012701:Serotonin; D005442:Flumazenil; D005680:gamma-Aminobutyric Acid; C090413:N-(2-(4-(2-methoxyphenyl)-1-piperazinyl)ethyl)-N-(2-pyridinyl)cyclohexanecarboxamide; C055669:spinosin
keywords: 5-HT(1A) receptor antagonist; Elevated plus maze; GABA(A) receptor antagonist; Light/dark box; Open field test; Spinosin