title: Microextraction method of medium and long chain fatty acids from milk.
abstract: The aim of this research was to develop a method for microextraction and quantification of long and medium chain fatty acids from milk. The proposed method was carried out on three steps: (1) lipids extraction from 10 μL of whole or skimmed milk containing omega-3 fatty acids (FAs) during 12, 24, 48, 72 and 96 h, in 2 mL chloroform: methanol mixture (2:1); (2) acid methylation of FAs; and (3) quantification of FAs methyl esters by gas chromatography. Using this method, the percentage recovery of FAs was higher (67% for whole and 85% for skimmed milk) compared with AOAC method 905.02 recovery (49% and 83%, respectively). Good reproducibility and repeatability (<3%) were obtained. The method developed can be useful for researching and in routine quality control. Additionally, it is simpler, faster and cheaper than the reference procedure since it requires minimum sample and solvents volume as well as fewer steps.
authors.affiliation: Centro de Investigaciones Químicas, Universidad Autónoma del Estado de Hidalgo, Carretera Pachuca-Tulancingo, km 4.5, C. P. 42184 Mineral de la Reforma, Hgo., Mexico. Electronic address: anorvej@uaeh.edu.mx.
authors.forename: Javier
authors.initials: J
authors.lastname: Añorve-Morga
authors.affiliation: Centro de Investigaciones Químicas, Universidad Autónoma del Estado de Hidalgo, Carretera Pachuca-Tulancingo, km 4.5, C. P. 42184 Mineral de la Reforma, Hgo., Mexico.
authors.forename: Araceli
authors.initials: A
authors.lastname: Castañeda-Ovando
authors.affiliation: Laboratorio de Higiene, Inspección y Control de los Alimentos, Departamento de Química Analítica, Nutrición y Bromatología, Universidad de Santiago de Compostela, Aguas Ferreas s/n, 27002 Lugo, Spain.
authors.forename: Alberto
authors.initials: A
authors.lastname: Cepeda-Saez
authors.affiliation: Departamento de Bioquímica, Facultad de Ciencias Naturales, Exactas y Tecnología, Universidad de Panamá, Ave. Manuel E. Bautista, Panama, Panama.
authors.forename: Armando Durant
authors.initials: AD
authors.lastname: Archibold
authors.affiliation: Centro de Investigaciones Químicas, Universidad Autónoma del Estado de Hidalgo, Carretera Pachuca-Tulancingo, km 4.5, C. P. 42184 Mineral de la Reforma, Hgo., Mexico.
authors.forename: Judith
authors.initials: J
authors.lastname: Jaimez-Ordaz
authors.affiliation: Centro de Investigaciones Químicas, Universidad Autónoma del Estado de Hidalgo, Carretera Pachuca-Tulancingo, km 4.5, C. P. 42184 Mineral de la Reforma, Hgo., Mexico.
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Contreras-López
authors.affiliation: Centro de Investigaciones Químicas, Universidad Autónoma del Estado de Hidalgo, Carretera Pachuca-Tulancingo, km 4.5, C. P. 42184 Mineral de la Reforma, Hgo., Mexico.
authors.forename: Luis Guillermo
authors.initials: LG
authors.lastname: González-Olivares
authors.affiliation: Departamento de Bioquímica y Biología Molecular, Facultad de Veterinaria, Universidad de Santiago de Compostela, Aguas Ferreas s/n, 27002 Lugo, Spain.
authors.forename: José Luis
authors.initials: JL
authors.lastname: Rodríguez-Rodríguez
mesh_terms: D000818:Animals; D002849:Chromatography, Gas; D005227:Fatty Acids / Q000032:analysis*; D005229:Fatty Acids, Monounsaturated / Q000032:analysis*; D015525:Fatty Acids, Omega-3 / Q000032:analysis*; D043371:Fatty Acids, Omega-6 / Q000032:analysis*; D008892:Milk / Q000737:chemistry*; D015203:Reproducibility of Results
chemical_list: D005227:Fatty Acids; D005229:Fatty Acids, Monounsaturated; D015525:Fatty Acids, Omega-3; D043371:Fatty Acids, Omega-6
keywords: Fatty acid; Microextraction method; Milk fat; Omega-3