title: MicroRNA-505 identified from patients with essential hypertension impairs endothelial cell migration and tube formation.
abstract: OBJECTIVE
  MicroRNAs are potent regulators of gene expression and may serve as disease markers. This study aimed to identify the plasma microRNA signature in hypertensive patients, which may help better understand the mechanisms underlying the pathogenesis of hypertension and target organ impairment.
  
  
  RESULTS
  Plasma samples from three independent cohorts were analyzed to identify circulating microRNA candidates associated with hypertension in patients. The results revealed that the plasma level of hsa-miR-505, a previously reported tumor suppressive microRNA, was significantly elevated in hypertensive patients. Further studies were carried out in endothelial cells to elucidate the functional significance of the enhanced level of hsa-miR-505. The results showed that hsa-miR-505 expression markedly impaired the migration and tube formation of all three types of endothelial cells examined. Moreover, gene expression analyses and luciferase reporter assay revealed that FGF18, a proangiogenic factor, is a target directly regulated by hsa-miR-505 in endothelial cells, which may in part underlie the function of hsa-miR-505 in angiogenic processes.
  
  
  CONCLUSIONS
  Our findings indicate that hsa-miR-505 is a novel circulating signature of hypertension, which may play a role in angiogenesis. Our results provide mechanistic insights into hypertension-associated pathogenesis and point hsa-miR-505 as a potential target for intervention of hypertension.
authors.affiliation: Yueyang Hospital and Clinical Research Institute of Integrative Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200437, China.
authors.forename: Qinbo
authors.initials: Q
authors.lastname: Yang
authors.affiliation: Yueyang Hospital and Clinical Research Institute of Integrative Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200437, China.
authors.forename: Chenglin
authors.initials: C
authors.lastname: Jia
authors.affiliation: Yueyang Hospital and Clinical Research Institute of Integrative Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200437, China.
authors.forename: Peiwei
authors.initials: P
authors.lastname: Wang
authors.affiliation: Yueyang Hospital and Clinical Research Institute of Integrative Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200437, China.
authors.forename: Minqi
authors.initials: M
authors.lastname: Xiong
authors.affiliation: Yueyang Hospital and Clinical Research Institute of Integrative Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200437, China.
authors.forename: Jingang
authors.initials: J
authors.lastname: Cui
authors.affiliation: Yueyang Hospital and Clinical Research Institute of Integrative Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200437, China.
authors.forename: Li
authors.initials: L
authors.lastname: Li
authors.affiliation: Yueyang Hospital and Clinical Research Institute of Integrative Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200437, China.
authors.forename: Wenjian
authors.initials: W
authors.lastname: Wang
authors.affiliation: Cyrus Tang Hematology Center, Soochow University, Suzhou 215123, China; Molecular Cardiology, Cleveland Clinic, Cleveland, OH 44195, USA.
authors.forename: Qingyu
authors.initials: Q
authors.lastname: Wu
authors.affiliation: Yueyang Hospital and Clinical Research Institute of Integrative Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200437, China. Electronic address: chenyu6639@gmail.com.
authors.forename: Yu
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Yueyang Hospital and Clinical Research Institute of Integrative Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200437, China. Electronic address: zhangteng501@hotmail.com.
authors.forename: Teng
authors.initials: T
authors.lastname: Zhang
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000097:blood; D002465:Cell Movement / Q000502:physiology*; D002478:Cells, Cultured; D015331:Cohort Studies; D042783:Endothelial Cells / Q000378:metabolism*; D000075222:Essential Hypertension; D061307:Human Umbilical Vein Endothelial Cells / Q000378:metabolism; D006801:Humans; D006973:Hypertension / Q000097:blood* / Q000175:diagnosis*; D008297:Male; D035683:MicroRNAs / Q000096:biosynthesis / Q000097:blood*; D008875:Middle Aged; D054259:Neural Tube / Q000254:growth & development / Q000378:metabolism*
chemical_list: D015415:Biomarkers; C574053:MIRN505 microRNA, human; D035683:MicroRNAs
keywords: Angiogenesis; Endothelial cell; FGF18; Hypertension; MicroRNA