title: Flux balance analysis of different carbon source fermentation with hydrogen producing Clostridium butyricum using Cell Net Analyzer.
abstract: A metabolic network model for Clostridium butyricum was developed using six different carbon sources (sucrose, fructose, galactose, mannose, trehalose and ribose) to study the fermentative H2 production. The model was used for investigation of H2 production and the ability of growth on different substrates to predict the maximum abilities for fermentative H2 production that each substrate can support. NADH fluxes were calculated by the model as an important cofactor affecting on H2 production. Butyrate and acetate production were used as model assumptions and biomass formation was chosen as the objective function for flux analysis calculations. Among the substrates selected, sucrose and trehalose supported the maximum growth and H2 yields. The Cell Net Analyzer metabolic network model developed for H2 estimation revealed good correlation with experimental data and could be further used to study the effect of environmental conditions and substrates concentration on H2 yield.
authors.affiliation: Biotechnology Group, Department of Chemical Engineering, Islamic Azad University of Iran, Science and Research Branch, Tehran, Iran. Electronic address: r.rafieenia@gmail.com.
authors.forename: Razieh
authors.initials: R
authors.lastname: Rafieenia
authors.affiliation: Great Lakes Institute for Environmental Research, University of Windsor, 401 Sunset Ave., Windsor, Ontario N9B 3P4, Canada. Electronic address: Chaganti@uwindsor.ca.
authors.forename: Subba Rao
authors.initials: SR
authors.lastname: Chaganti
mesh_terms: D000085:Acetates / Q000378:metabolism; D018533:Biomass; D002087:Butyrates / Q000378:metabolism; D002244:Carbon / Q000378:metabolism*; D046972:Clostridium butyricum / Q000378:metabolism*; D005285:Fermentation; D006859:Hydrogen / Q000378:metabolism*; D053858:Metabolic Networks and Pathways; D008954:Models, Biological; D013395:Sucrose / Q000378:metabolism; D014199:Trehalose / Q000378:metabolism
chemical_list: D000085:Acetates; D002087:Butyrates; D013395:Sucrose; D002244:Carbon; D006859:Hydrogen; D014199:Trehalose
keywords: Carbon source; Cell Net Analyzer; Clostridium butyricum; Flux balance analysis; Hydrogen