title: Phytochemical Constituents and Toxicity of Duguetia furfuracea Hydroalcoholic Extract in Drosophila melanogaster.
abstract: Duguetia furfuracea is frequently used as a medicinal plant in Brazil. However, studies have evidenced its cytotoxic, bactericide, and antitumor activities. In the present study we aimed to evaluate the potential toxicity of hydroalcoholic leaves extracts of D. furfuracea (HEDF) in a Drosophila melanogaster model. Toxicity was assessed as changes in locomotor performance, mitochondrial activity, oxidative stress, MAPKs phosphorylation, and apoptosis induction after exposure to HEDF concentrations (1-50 mg/mL) for 7 days. The phytoconstituents of the plant were screened for the presence of alkaloids, tannins, xanthones, chalcones, flavonoids, aurones, and phenolic acids. Exposure of adult flies to HEDF caused mitochondrial dysfunction, overproduction of ROS, and alterations in the activity of detoxifying enzymes GST, SOD and CAT. Induction of ERK phosphorylation and PARP cleavage was also observed, indicating occurrence of HEDF-induced cell stress and apoptotic cell death. In parallel, alterations in cholinesterase activity and impairments in negative geotaxis behavior were observed. Our study draws attention to the indiscriminate use of this plant by population and suggests oxidative stress as a major mechanism underlying its toxicity.
authors.affiliation: Departamento de Química Biológica, Universidade Regional do Cariri, 63100-000 Crato, CE, Brazil ; Departamento de Química, Programa de Pós-Graduação em Bioquímica Toxicológica, Universidade Federal de Santa Maria, 97105-900 Santa Maria, RS, Brazil.
authors.forename: Francisca
authors.initials: F
authors.lastname: Valéria Soares de Araújo Pinho
authors.affiliation: Centro Interdisciplinar de Pesquisa em Biotecnologia, Universidade Federal do Pampa, Campus São Gabriel, 97300-000 São Gabriel, RS, Brazil.
authors.forename: Gustavo
authors.initials: G
authors.lastname: Felipe da Silva
authors.affiliation: Centro Interdisciplinar de Pesquisa em Biotecnologia, Universidade Federal do Pampa, Campus São Gabriel, 97300-000 São Gabriel, RS, Brazil.
authors.forename: Giulianna
authors.initials: G
authors.lastname: Echeverria Macedo
authors.affiliation: Centro Interdisciplinar de Pesquisa em Biotecnologia, Universidade Federal do Pampa, Campus São Gabriel, 97300-000 São Gabriel, RS, Brazil.
authors.forename: Katiane
authors.initials: K
authors.lastname: Raquel Muller
authors.affiliation: Centro Interdisciplinar de Pesquisa em Biotecnologia, Universidade Federal do Pampa, Campus São Gabriel, 97300-000 São Gabriel, RS, Brazil.
authors.forename: Illana
authors.initials: I
authors.lastname: Kemmerich Martins
authors.affiliation: Centro Interdisciplinar de Pesquisa em Biotecnologia, Universidade Federal do Pampa, Campus São Gabriel, 97300-000 São Gabriel, RS, Brazil.
authors.forename: Ana Paula
authors.identifier: 0000-0002-8484-8492
authors.initials: AP
authors.lastname: Lausmann Ternes
authors.affiliation: Departamento de Química Biológica, Universidade Regional do Cariri, 63100-000 Crato, CE, Brazil.
authors.forename: José Galberto
authors.initials: JG
authors.lastname: Martins da Costa
authors.affiliation: Departamento de Farmácia Industrial, Laboratório de Pesquisa em Fitoquímica, Universidade Federal de Santa Maria, Prédio 26, Sala 1115, 97105-900 Santa Maria, RS, Brazil.
authors.forename: Margareth Linde
authors.initials: ML
authors.lastname: Athayde
authors.affiliation: Departamento de Farmácia Industrial, Laboratório de Pesquisa em Fitoquímica, Universidade Federal de Santa Maria, Prédio 26, Sala 1115, 97105-900 Santa Maria, RS, Brazil.
authors.forename: Aline Augusti
authors.initials: AA
authors.lastname: Boligon
authors.affiliation: Departamento de Química, Programa de Pós-Graduação em Bioquímica Toxicológica, Universidade Federal de Santa Maria, 97105-900 Santa Maria, RS, Brazil ; Departamento de Bioquímica, Instituto de Ciências Básica da Saúde, Universidade Federal do Rio Grande do Sul, 90035-003 Porto Alegre, RS, Brazil.
authors.forename: Jean Paul
authors.initials: JP
authors.lastname: Kamdem
authors.affiliation: Centro Interdisciplinar de Pesquisa em Biotecnologia, Universidade Federal do Pampa, Campus São Gabriel, 97300-000 São Gabriel, RS, Brazil.
authors.forename: Jeferson Luis
authors.identifier: 0000-0002-3874-8618
authors.initials: JL
authors.lastname: Franco
authors.affiliation: Departamento de Química Biológica, Universidade Regional do Cariri, 63100-000 Crato, CE, Brazil.
authors.forename: Irwin
authors.initials: I
authors.lastname: Rose Alencar de Menezes
authors.affiliation: Centro Interdisciplinar de Pesquisa em Biotecnologia, Universidade Federal do Pampa, Campus São Gabriel, 97300-000 São Gabriel, RS, Brazil.
authors.forename: Thaís
authors.identifier: 0000-0002-6491-5854
authors.initials: T
authors.lastname: Posser