title: Respiratory complex I from Escherichia coli does not transport Na(+) in the absence of its NuoL subunit.
abstract: We investigated H(+) and Na(+) transport by complex I from Escherichia coli devoid of the NuoL subunit, which is probably part of the ion translocating machinery. We observed that complex I devoid of the NuoL subunit still translocates H(+), although to a smaller extension than the complete version of complex I, but does not transport Na(+). Our results unequivocally reinforce the observation that E. coli complex I transports Na(+) in the opposite direction to that of the H+ and show that NuoL subunit is involved in the translocation of both ions by complex I.
authors.affiliation: Instituto de Tecnologia Química e Biológica – António Xavier, Universidade Nova de Lisboa, Av. da Republica EAN, 2780-157 Oeiras, Portugal.
authors.forename: Bruno C
authors.initials: BC
authors.lastname: Marreiros
authors.forename: Ana P
authors.initials: AP
authors.lastname: Batista
authors.forename: Manuela M
authors.initials: MM
authors.lastname: Pereira
mesh_terms: D001692:Biological Transport; D002462:Cell Membrane / Q000378:metabolism; D004926:Escherichia coli / Q000166:cytology / Q000201:enzymology*; D029968:Escherichia coli Proteins / Q000737:chemistry / Q000378:metabolism*; D008958:Models, Molecular; D009245:NADH Dehydrogenase / Q000737:chemistry / Q000172:deficiency* / Q000378:metabolism*; D011487:Protein Conformation; D021122:Protein Subunits / Q000737:chemistry / Q000172:deficiency* / Q000378:metabolism*; D011522:Protons; D012964:Sodium / Q000378:metabolism*
chemical_list: D029968:Escherichia coli Proteins; D021122:Protein Subunits; D011522:Protons; D012964:Sodium; D009245:NADH Dehydrogenase; C479743:NuoL protein, E coli