title: Evaluation of sorption capacity of antibiotics and antibacterial properties of a cyclodextrin-polymer functionalized hydroxyapatite-coated titanium hip prosthesis.
abstract: Infection still present as one of common complications after total hip replacement (∼2.5%), which may cause serious outcomes. For preventing such risk, loading antibiotics onto implants for increasing local drug concentration at targeted sites could be a solution. This study aims at modifying the surface of hydroxyapatite (HA) coated titanium hip implant material (Ti-HA) with polymer of cyclodextrin (polyCD) for loading antibiotics, to achieve a sustained local drug delivery. Two widely applied antibiotics (tobramycin and rifampicin) in orthopedic surgery were loaded alone or in combination. The drug adsorption isotherm, drug release kinetics and drug's efficacy were thoroughly investigated. The results proved that polyCD coating significantly improved the affinity of both drugs to Ti-HA surface, while the mechanism of drug-polyCD interaction varies from the nature of drug, courtesy of the structural complex of polyCD. The advantage of dual-drug loading was highlighted by its strong efficacy against both Staphylococcus aureus and Enterobacter cloacae, which overcomes the limitation of mono-drug loading for an effective treatment against both bacterial strains. The prolonged antibacterial activity of antibiotic loaded Ti-HA-polyCD samples confirmed that polyCD could be a promising drug-delivery system, for sustained antibiotics release or other potential applications e.g., antimitotic agent release.
authors.affiliation: Université Lille Nord De France, 59000 Lille, France; INSERM U1008, Controlled Drug Delivery System and Biomaterials, University Lille 2, F-59006 Lille, France.
authors.forename: Mariam
authors.initials: M
authors.lastname: Taha
authors.affiliation: Université Lille Nord De France, 59000 Lille, France; INSERM U1008, Controlled Drug Delivery System and Biomaterials, University Lille 2, F-59006 Lille, France.
authors.forename: Feng
authors.initials: F
authors.lastname: Chai
authors.affiliation: Université Lille Nord De France, 59000 Lille, France; INSERM U1008, Controlled Drug Delivery System and Biomaterials, University Lille 2, F-59006 Lille, France. Electronic address: nicolas.blanchemain@univ-lille2.fr.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Blanchemain
authors.affiliation: Université Lille Nord De France, 59000 Lille, France; INSERM U995, Laboratoire de Bactériologie, University Lille 2, 59006 Lille, France.
authors.forename: Christel
authors.initials: C
authors.lastname: Neut
authors.affiliation: BJR-France, R&D Department, 1 Bis rue Saint Roch, 62170 Bréxent-Énocq, France.
authors.forename: Michel
authors.initials: M
authors.lastname: Goube
authors.affiliation: Université Lille Nord De France, 59000 Lille, France; INSERM U1008, Controlled Drug Delivery System and Biomaterials, University Lille 2, F-59006 Lille, France.
authors.forename: Mickael
authors.initials: M
authors.lastname: Maton
authors.affiliation: Université Lille Nord De France, 59000 Lille, France; UMET - Ingénierie des Systèmes Polymères, University Lille 1, 59655 Villeneuve d'Ascq, France.
authors.forename: Bernard
authors.initials: B
authors.lastname: Martel
authors.affiliation: Université Lille Nord De France, 59000 Lille, France; INSERM U1008, Controlled Drug Delivery System and Biomaterials, University Lille 2, F-59006 Lille, France.
authors.forename: Hartmut F
authors.initials: HF
authors.lastname: Hildebrand
mesh_terms: D000327:Adsorption; D000900:Anti-Bacterial Agents / Q000008:administration & dosage* / Q000494:pharmacology; D003692:Delayed-Action Preparations; D004337:Drug Carriers / Q000737:chemistry*; D004338:Drug Combinations; D065546:Drug Liberation; D016972:Enterobacter cloacae / Q000187:drug effects; D006622:Hip Prosthesis / Q000382:microbiology*; D006882:Hydroxyapatites / Q000737:chemistry*; D012293:Rifampin / Q000008:administration & dosage / Q000494:pharmacology; D013211:Staphylococcus aureus / Q000187:drug effects; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry*; D014031:Tobramycin / Q000008:administration & dosage / Q000494:pharmacology; D047392:beta-Cyclodextrins / Q000737:chemistry*
chemical_list: D000900:Anti-Bacterial Agents; D003692:Delayed-Action Preparations; D004337:Drug Carriers; D004338:Drug Combinations; D006882:Hydroxyapatites; D047392:beta-Cyclodextrins; C098170:hydroxyapatite-titanium alloy; C108732:methyl-beta-cyclodextrin; D014025:Titanium; D012293:Rifampin; D014031:Tobramycin
keywords: Antibiotics; Cyclodextrin; Drug delivery system; Hip prosthesis; Infection