title: Separation and purification of amygdalin from thinned bayberry kernels by macroporous adsorption resins.
abstract: To utilize the low-value thinned bayberry (Myrica rubra Sieb. et Zucc) kernels (TBKs) waste, an efficient method using macroporous adsorption resins (MARs) for separation and purification of amygdalin from TBKs crude extracts was developed. An aqueous crude sample was prepared from a methanol TBK extract, followed by resin separation. A series of MARs were initially screened for adsorption/desorption of amygdalin in the extract, and D101 was selected for characterization and method development. The static adsorption data of amygdalin on D101 was best fitted to the pseudo-second-order kinetics model. The solute affinity toward D101 at 30 °C was described and the equilibrium experimental data were well-fitted to Langmuir and Freundlich isotherms. Through one cycle of dynamic adsorption/desorption, the purity of amygdalin in the extract, determined by HPLC, increased about 17-fold from 4.8% to 82.0%, with 77.9% recovery. The results suggested that D101 resin effectively separate amygdalin from TBKs.
authors.affiliation: Key Laboratory of Fruits and Vegetables Postharvest and Processing of Zhejiang Province, Institute of Food Science, Zhejiang Academy of Agricultural Science, Hangzhou 310021, PR China; Zhejiang Normal University, Jinhua 321000, PR China.
authors.forename: Tao
authors.initials: T
authors.lastname: Wang
authors.affiliation: Key Laboratory of Fruits and Vegetables Postharvest and Processing of Zhejiang Province, Institute of Food Science, Zhejiang Academy of Agricultural Science, Hangzhou 310021, PR China. Electronic address: lushengmin@hotmail.com.
authors.forename: Shengmin
authors.initials: S
authors.lastname: Lu
authors.affiliation: Key Laboratory of Fruits and Vegetables Postharvest and Processing of Zhejiang Province, Institute of Food Science, Zhejiang Academy of Agricultural Science, Hangzhou 310021, PR China.
authors.forename: Qile
authors.initials: Q
authors.lastname: Xia
authors.affiliation: Food Science & Technology Program, School of Public Health, Faculty of Health Sciences, International Institute of Agri-Food Security (IIAFS), Curtin University, GPO Box U1987, Perth 6845, WA, Australia.
authors.forename: Zhongxiang
authors.initials: Z
authors.lastname: Fang
authors.affiliation: Food Science & Technology Program, School of Public Health, Faculty of Health Sciences, International Institute of Agri-Food Security (IIAFS), Curtin University, GPO Box U1987, Perth 6845, WA, Australia.
authors.forename: Stuart
authors.initials: S
authors.lastname: Johnson
mesh_terms: D000327:Adsorption; D000678:Amygdalin / Q000302:isolation & purification*; D000972:Antineoplastic Agents, Phytogenic / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D031641:Myrica / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry*; D016062:Porosity; D012117:Resins, Synthetic / Q000737:chemistry*
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D010936:Plant Extracts; D012117:Resins, Synthetic; D000678:Amygdalin
keywords: Amygdalin; Bayberry; Macroporous adsorption resins (MARs); Purification; Thinned bayberry kernels (TBKs)