title: Production of a lignocellulolytic enzyme system for simultaneous bio-delignification and saccharification of corn stover employing co-culture of fungi.
abstract: Aiming at improving the efficiency of transferring corn stover into sugars, an efficient lignocellulolytic enzyme system was developed and investigated by co-cultivation of the Coprinus comatus with Trichoderma reesei in a single bioreactor. The results showed that the lignocellulolytic enzyme activities of the co-culture exceeded that of the monoculture, suggesting synergistic interaction between two fungi. The highest laccase activity from the co-culture was 2.6-fold increase over that of the C. comatus monoculture and reached a peak 3days earlier. The maximum delignification obtained was 66.5% and about 82% of the original polysaccharides were converted into fermentable sugars by simultaneous bio-delignification and saccharification process. Correlation analysis showed that sugar yields were directly proportional to the lignin degradation. Our results suggested that co-fungi cultivation was a valuable technique for corn stover bioconversion, which could produce high efficiency of lignocellulolytic enzyme system as a cheaper alternative to commercial enzymes for industrial utilization.
authors.affiliation: College of Environmental and Chemical Engineering, Dalian University, Dalian 116622, PR China. Electronic address: makedongdl@yahoo.co.jp.
authors.forename: Kedong
authors.initials: K
authors.lastname: Ma
authors.affiliation: Key Laboratory of Microbial Resources (Ministry of Agriculture, China), Institute of Agricultural Resources and Regional Planning, CAAS, Beijing 100081, PR China. Electronic address: ruanzhiyong@caas.cn.
authors.forename: Zhiyong
authors.initials: Z
authors.lastname: Ruan
mesh_terms: D019149:Bioreactors / Q000382:microbiology; D001709:Biotechnology / Q000295:instrumentation / Q000379:methods*; D002241:Carbohydrates / Q000096:biosynthesis; D018920:Coculture Techniques; D003302:Coprinus / Q000166:cytology / Q000201:enzymology*; D004798:Enzymes / Q000737:chemistry / Q000378:metabolism*; D042845:Laccase / Q000378:metabolism; D008031:Lignin / Q000378:metabolism*; D018520:Plant Shoots / Q000737:chemistry / Q000378:metabolism; D011134:Polysaccharides / Q000378:metabolism; D014242:Trichoderma / Q000166:cytology / Q000201:enzymology*; D003313:Zea mays / Q000737:chemistry / Q000378:metabolism*
chemical_list: D002241:Carbohydrates; D004798:Enzymes; D011134:Polysaccharides; D008031:Lignin; D042845:Laccase
keywords: Bio-delignification; Co-culture; Corn stover; Hydrolytic enzymes; Ligninolytic enzymes