title: Tritium-labeled (E,E)-2,5-bis(4'-hydroxy-3'-carboxystyryl)benzene as a probe for β-amyloid fibrils.
abstract: Accumulation of Aβ in the brains of Alzheimer disease (AD) patients reflects an imbalance between Aβ production and clearance from their brains. Alternative cleavage of amyloid precursor protein (APP) by processing proteases generates soluble APP fragments including the neurotoxic amyloid Aβ40 and Aβ42 peptides that assemble into fibrils and form plaques. Plaque-buildup occurs over an extended time-frame, and the early detection and modulation of plaque formation are areas of active research. Radiolabeled probes for the detection of amyloid plaques and fibrils in living subjects are important for noninvasive evaluation of AD diagnosis, progression, and differentiation of AD from other neurodegenerative diseases and age-related cognitive decline. Tritium-labeled (E,E)-1-[(3)H]-2,5-bis(4'-hydroxy-3'-carbomethoxystyryl)benzene possesses an improved level of chemical stability relative to a previously reported radioiodinated analog for radiometric quantification of Aβ plaque and tau pathology in brain tissue and in vitro studies with synthetic Aβ and tau fibrils.
authors.affiliation: Department of Molecular and Cellular Biochemistry, College of Medicine, University of Kentucky, Lexington, KY 40536-0509, United States; Sanders-Brown Center on Aging, University of Kentucky, Lexington, KY 40536-0230, United States.
authors.forename: Sergey V
authors.initials: SV
authors.lastname: Matveev
authors.affiliation: Department of Molecular and Cellular Biochemistry, College of Medicine, University of Kentucky, Lexington, KY 40536-0509, United States; Center for Pharmaceutical Research and Innovation, College of Pharmacy, University of Kentucky, Lexington, KY 40536-0596, United States.
authors.forename: Stefan
authors.initials: S
authors.lastname: Kwiatkowski
authors.affiliation: Department of Molecular and Cellular Biochemistry, College of Medicine, University of Kentucky, Lexington, KY 40536-0509, United States; Center for Pharmaceutical Research and Innovation, College of Pharmacy, University of Kentucky, Lexington, KY 40536-0596, United States.
authors.forename: Vitaliy M
authors.initials: VM
authors.lastname: Sviripa
authors.affiliation: ViTrax Radiochemicals, 660 S. Jefferson Street, Unit E, Placentia, CA 92870, United States.
authors.forename: Robert C
authors.initials: RC
authors.lastname: Fazio
authors.affiliation: Department of Molecular and Cellular Biochemistry, College of Medicine, University of Kentucky, Lexington, KY 40536-0509, United States; Center for Pharmaceutical Research and Innovation, College of Pharmacy, University of Kentucky, Lexington, KY 40536-0596, United States; Lucille Parker Markey Cancer Center, University of Kentucky, Lexington, KY 40536-0093, United States. Electronic address: dwatt@uky.edu.
authors.forename: David S
authors.initials: DS
authors.lastname: Watt
authors.affiliation: Department of Molecular and Cellular Biochemistry, College of Medicine, University of Kentucky, Lexington, KY 40536-0509, United States; Sanders-Brown Center on Aging, University of Kentucky, Lexington, KY 40536-0230, United States. Electronic address: harry.levine@uky.edu.
authors.forename: Harry
authors.initials: H
authors.lastname: LeVine
mesh_terms: D000544:Alzheimer Disease / Q000378:metabolism*; D000682:Amyloid / Q000378:metabolism*; D001554:Benzene / Q000378:metabolism*; D015394:Molecular Structure; D014316:Tritium / Q000378:metabolism*
chemical_list: D000682:Amyloid; D014316:Tritium; D001554:Benzene
keywords: Alzheimer’s disease; Detection of amyloid fibrils; Tritium-labeled probe; β-Amyloid