title: Review of in vivo studies of dimethyl sulfoxide cryopreserved platelets.
abstract: A literature review was conducted to assess the efficacy and safety of dimethyl sulfoxide (DMSO) cryopreserved platelets for potential military use. In vivo DMSO cryopreserved platelet studies published between 1972 and June of 2013 were reviewed. Assessed were the methods of cryopreservation, posttransfusion platelet responses, prevention or control of bleeding, and adverse events. Using the Department of Defense's preferred 6% DMSO cryopreservation method with centrifugation to remove the DMSO plasma before freezing at -65°C and no postthaw wash, mean radiolabeled platelet recoveries in 32 normal subjects were 33% ± 10% (52% ± 12% of the same subject's fresh platelet recoveries), and survivals were 7.5 ± 1.2 days (89% ± 15% of fresh platelet survivals). Using a variety of methods to freeze autologous platelets from 178 normal subjects, mean radiolabeled platelet recoveries were consistently 39% ± 9%, and survivals, 7.4 ± 1.4 days. More than 3000 cryopreserved platelet transfusions were given to 1334 patients. There were 19 hematology/oncology patient studies, and, in 9, mean 1-hour corrected count increments were 11 100 ± 3600 (range, 5700-15 800) after cryopreserved autologous platelet transfusions. In 5 studies, bleeding times improved after transfusion; in 3, there was either no improvement or a variable response. In 4 studies, there was immediate cessation of bleeding after transfusion; in 3 studies, patients being supported only with cryopreserved platelets had no bleeding. In 1 cardiopulmonary bypass study, cryopreserved platelets resulted in significantly less bleeding vs standard platelets. In 3 trauma studies, cryopreserved platelets were hemostatically effective. No significant adverse events were reported in any study. In summary, cryopreserved platelets have platelet recoveries that are about half of fresh platelets, but survivals are only minimally reduced. The platelets appear hemostatically effective and have no significant adverse events.
authors.affiliation: Puget Sound Blood Center Seattle, WA; University of Washington School of Medicine, Seattle, WA. Electronic address: sjslichter@psbc.org.
authors.forename: Sherrill J
authors.initials: SJ
authors.lastname: Slichter
authors.affiliation: Puget Sound Blood Center Seattle, WA.
authors.forename: Melinh
authors.initials: M
authors.lastname: Jones
authors.affiliation: Fast-Track Drugs & Biologics, LLC, North Potomac, MD.
authors.forename: Janet
authors.initials: J
authors.lastname: Ransom
authors.affiliation: Puget Sound Blood Center Seattle, WA.
authors.forename: Irena
authors.initials: I
authors.lastname: Gettinger
authors.affiliation: Puget Sound Blood Center Seattle, WA.
authors.forename: Mary Kay
authors.initials: MK
authors.lastname: Jones
authors.affiliation: Puget Sound Blood Center Seattle, WA.
authors.forename: Todd
authors.initials: T
authors.lastname: Christoffel
authors.affiliation: Puget Sound Blood Center Seattle, WA.
authors.forename: Esther
authors.initials: E
authors.lastname: Pellham
authors.affiliation: Puget Sound Blood Center Seattle, WA.
authors.forename: S Lawrence
authors.initials: SL
authors.lastname: Bailey
authors.affiliation: Puget Sound Blood Center Seattle, WA.
authors.forename: Jill
authors.initials: J
authors.lastname: Corson
authors.affiliation: Puget Sound Blood Center Seattle, WA.
authors.forename: Doug
authors.initials: D
authors.lastname: Bolgiano
mesh_terms: D001792:Blood Platelets / Q000166:cytology*; D001793:Blood Preservation / Q000379:methods; D002470:Cell Survival; D015925:Cryopreservation / Q000379:methods*; D004121:Dimethyl Sulfoxide / Q000737:chemistry*; D005720:Gamma Rays; D006487:Hemostasis; D006801:Humans; D017713:Platelet Transfusion / Q000379:methods; D013696:Temperature
chemical_list: D004121:Dimethyl Sulfoxide
keywords: Cryopreservation; Dimethyl sulfoxide; Platelet transfusions; Platelets