title: An UPF3-based nonsense-mediated decay in Paramecium.
abstract: Nonsense-mediated decay recognises mRNAs containing premature termination codons. One of its components, UPF3, is a molecular link bridging through its binding to the exon junction complex nonsense-mediated decay and splicing. In protists UPF3 has not been identified yet. We report that Paramecium tetraurelia bears an UPF3 gene and that it has a role in nonsense-mediated decay. Interestingly, the identified UPF3 has not conserved the essential amino acids required to bind the exon junction complex. Though, our data indicates that this ciliate bears genes coding for core proteins of the exon junction complex.
authors.affiliation: Departamento de Microbiología, Universidad de Sevilla, Av Reina Mercedes 6, 41012 Seville, Spain. Electronic address: juli_bio_87@hotmail.com.
authors.forename: Julia
authors.initials: J
authors.lastname: Contreras
authors.affiliation: Departamento de Microbiología, Universidad de Sevilla, Av Reina Mercedes 6, 41012 Seville, Spain. Electronic address: vsbegley@gmail.com.
authors.forename: Victoria
authors.initials: V
authors.lastname: Begley
authors.affiliation: Departamento de Microbiología, Universidad de Sevilla, Av Reina Mercedes 6, 41012 Seville, Spain. Electronic address: sanmacben@hotmail.es.
authors.forename: Sandra
authors.initials: S
authors.lastname: Macias
authors.affiliation: Departamento de Microbiología, Universidad de Sevilla, Av Reina Mercedes 6, 41012 Seville, Spain. Electronic address: evpolo@us.es.
authors.forename: Eduardo
authors.initials: E
authors.lastname: Villalobo
mesh_terms: D000595:Amino Acid Sequence; D018389:Codon, Nonsense; D008969:Molecular Sequence Data; D010247:Paramecium / Q000737:chemistry / Q000235:genetics*; D015800:Protozoan Proteins / Q000737:chemistry / Q000235:genetics*; D016415:Sequence Alignment
chemical_list: D018389:Codon, Nonsense; D015800:Protozoan Proteins
keywords: Exon junction complex; Nonsense-mediated decay; Paramecium tetraurelia; Premature termination codon; Splicing; UPF3