title: Ultrasonic pretreatment in lipase-catalyzed synthesis of structured lipids with high 1,3-dioleoyl-2-palmitoylglycerol content.
abstract: Production of structured lipid 1,3-dioleoyl-2-palmitoylglycerol (OPO), from tripalmitin (PPP) and oleic acid (OA) using lipases and ultrasonic pretreatment was conducted. Factors influencing both the ultrasonic conditions and enzymatic reaction were investigated. Optimum conditions could be attained with 6 min pretreatment time, 50% ultrasonic power, 3 s/9 s (work/pause) cycle of ultrasonic pulse, 1:8 PPP/OA molar ratio, 12% enzyme dosage and 50 °C temperature of. At the optimum conditions, the OPO yield of 51.8% could be achieved in 4h. Studies showed that the OPO content increased to 35.9% in 1h with ultrasonic pretreatment, in comparison to 4h without ultrasonic pretreatment. Reuse of Lipozyme RM IM for 10 cycles under ultrasonic irradiation did not cause essential damage to its lipase activity. Reaction kinetic model fitted well with the proposed Ping-Pong mechanism. The apparent kinetic constant (Vm'/K₂) of ultrasound pretreatment reaction was 2.52 times higher than the conventional mechanical stirring, indicating that ultrasound pretreatment enhanced the substrates affinity to the enzyme. This study confirmed that ultrasonic pretreatment was more efficient in OPO production than conventional mechanical agitation.
authors.affiliation: Institute of Oil Crops Research, Chinese Academy of Agricultural Sciences, The Key Lab for Biological Sciences of Oil Crops, Ministry of Agriculture - Hubei Key Laboratory of Lipid Chemistry and Nutrition, Wuhan, Hubei 430062, PR China.
authors.forename: Si-lei
authors.initials: SL
authors.lastname: Liu
authors.affiliation: Institute of Oil Crops Research, Chinese Academy of Agricultural Sciences, The Key Lab for Biological Sciences of Oil Crops, Ministry of Agriculture - Hubei Key Laboratory of Lipid Chemistry and Nutrition, Wuhan, Hubei 430062, PR China. Electronic address: dongxuyan@caas.cn.
authors.forename: Xu-yan
authors.initials: XY
authors.lastname: Dong
authors.affiliation: Institute of Oil Crops Research, Chinese Academy of Agricultural Sciences, The Key Lab for Biological Sciences of Oil Crops, Ministry of Agriculture - Hubei Key Laboratory of Lipid Chemistry and Nutrition, Wuhan, Hubei 430062, PR China.
authors.forename: Fang
authors.initials: F
authors.lastname: Wei
authors.affiliation: Institute of Oil Crops Research, Chinese Academy of Agricultural Sciences, The Key Lab for Biological Sciences of Oil Crops, Ministry of Agriculture - Hubei Key Laboratory of Lipid Chemistry and Nutrition, Wuhan, Hubei 430062, PR China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Wang
authors.affiliation: Institute of Oil Crops Research, Chinese Academy of Agricultural Sciences, The Key Lab for Biological Sciences of Oil Crops, Ministry of Agriculture - Hubei Key Laboratory of Lipid Chemistry and Nutrition, Wuhan, Hubei 430062, PR China.
authors.forename: Xin
authors.initials: X
authors.lastname: Lv
authors.affiliation: Institute of Oil Crops Research, Chinese Academy of Agricultural Sciences, The Key Lab for Biological Sciences of Oil Crops, Ministry of Agriculture - Hubei Key Laboratory of Lipid Chemistry and Nutrition, Wuhan, Hubei 430062, PR China.
authors.forename: Juan
authors.initials: J
authors.lastname: Zhong
authors.affiliation: Institute of Oil Crops Research, Chinese Academy of Agricultural Sciences, The Key Lab for Biological Sciences of Oil Crops, Ministry of Agriculture - Hubei Key Laboratory of Lipid Chemistry and Nutrition, Wuhan, Hubei 430062, PR China.
authors.forename: Lin
authors.initials: L
authors.lastname: Wu
authors.affiliation: School of Chemical Sciences, The University of Auckland, Auckland 1142, New Zealand.
authors.forename: Siew-young
authors.initials: SY
authors.lastname: Quek
authors.affiliation: Institute of Oil Crops Research, Chinese Academy of Agricultural Sciences, The Key Lab for Biological Sciences of Oil Crops, Ministry of Agriculture - Hubei Key Laboratory of Lipid Chemistry and Nutrition, Wuhan, Hubei 430062, PR China. Electronic address: chenhong@oilcrops.cn.
authors.forename: Hong
authors.initials: H
authors.lastname: Chen
mesh_terms: D055162:Biocatalysis; D060326:Chemistry Techniques, Synthetic; D005658:Fungi / Q000201:enzymology; D007700:Kinetics; D008049:Lipase / Q000737:chemistry / Q000378:metabolism*; D008956:Models, Chemical; D012834:Silver / Q000737:chemistry; D014280:Triglycerides / Q000138:chemical synthesis* / Q000737:chemistry*; D014465:Ultrasonics
chemical_list: D014280:Triglycerides; D012834:Silver; C093666:Lipozyme; D008049:Lipase; C095376:1,3-dioleoyl-2-palmitoylglycerol
keywords: 1,3-Dioleoyl-2-palmitoylglycerol; Acidolysis; Acyl migration; Lipase-catalyzed; Structured lipid; Ultrasonic pretreatment