title: Mode of encapsulation of linezolid by β-cyclodextrin and its role in bovine serum albumin binding.
abstract: We describe, in this article, the associative interaction between Linezolid and β-Cyclodextrin, and the influence of β-Cyclodextrin on Linezolid's binding to Bovine serum albumin. β-Cyclodextrin forms a 1:1 inclusion complex with Linezolid, with a binding constant value of 3.51×10(2)M(-1). The binding is studied using ultraviolet-visible absorption, fluorescence, nuclear magnetic resonance, and rotating-frame overhauser effect spectroscopic techniques. The amide substituent on the oxazolidinone ring of Linezolid is involved in its binding to β-Cyclodextrin. The binding of the Linezolid to bovine serum albumin, in the absence and the presence of β-Cyclodextrin, is studied by analyzing the fluorescence quenching and Förster resonance energy transfer. The Stern-Volmer quenching constant, the binding constant, and energy transfer occurring on the interaction of the Linezolid with BSA are found to be smaller in the presence of β-Cyclodextrin than in water.
authors.affiliation: Department of Chemistry, School of Science and Humanities, Karunya University, Coimbatore 641114, Tamil Nadu, India.
authors.forename: Sudha
authors.initials: S
authors.lastname: Natesan
authors.affiliation: Department of Chemistry, School of Science and Humanities, Karunya University, Coimbatore 641114, Tamil Nadu, India.
authors.forename: Chandrasekaran
authors.initials: C
authors.lastname: Sowrirajan
authors.affiliation: Department of Chemistry, School of Science and Humanities, Karunya University, Coimbatore 641114, Tamil Nadu, India.
authors.forename: Sameena
authors.initials: S
authors.lastname: Yousuf
authors.affiliation: Department of Chemistry, School of Science and Humanities, Karunya University, Coimbatore 641114, Tamil Nadu, India. Electronic address: drisraelenoch@gmail.com.
authors.forename: Israel V M V
authors.initials: IV
authors.lastname: Enoch
mesh_terms: D000081:Acetamides / Q000737:chemistry*; D000890:Anti-Infective Agents / Q000737:chemistry; D000069349:Linezolid; D062105:Molecular Docking Simulation; D023303:Oxazolidinones / Q000737:chemistry*; D011500:Protein Synthesis Inhibitors / Q000737:chemistry; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D047392:beta-Cyclodextrins / Q000737:chemistry*
chemical_list: D000081:Acetamides; D000890:Anti-Infective Agents; D023303:Oxazolidinones; D011500:Protein Synthesis Inhibitors; D047392:beta-Cyclodextrins; D012710:Serum Albumin, Bovine; D000069349:Linezolid; C031215:betadex
keywords: 2D ROESY; Bovine serum albumin; FRET; Fluorescence; Linezolid; β-Cyclodextrin