title: Y-shaped ligand-driven gold nanoparticles for highly efficient tumoral uptake and photothermal ablation.
abstract: We report functional gold nanoparticles (AuNP) with antibody-like ligands. These particles consist of Y-shaped ligands and AuNP. Transferrin (Tf) and Tat peptide were linked to each head of a Y-shaped poly(ethylene glycol) (PEG)-containing dopamine at one tail site. Also, Y-shaped ligands (with Tf and Tat peptide) were anchored to the surface of the AuNP as the result of noncovalent conjugation of dopamine and the AuNP. Interestingly, the partial shielding of Tat peptides by large Tf molecules rather improved Tf-mediated endocytosis of the AuNP, while minimizing the natural nonspecific cell interaction of Tat peptides. This system resulted in highly improved in vitro/in vivo tumor-selective uptake over AuNP bearing a single ligand (Tf or Tat peptides). Furthermore, this system resulted in significant enhancement of in vivo photothermal tumor cell ablation under light-irradiation conditions for AuNP. We believe that this design is a promising method to easily modify conventional antibodies or ligands to improve their disease-recognition ability.
authors.affiliation: Department of Biotechnology, The Catholic University of Korea , 43-1 Yeokgok 2-dong, Wonmi-gu, Bucheon, Gyeonggi-do 420-743, Republic of Korea.
authors.forename: Ung Yeol
authors.initials: UY
authors.lastname: Lee
authors.forename: Yu Seok
authors.initials: YS
authors.lastname: Youn
authors.forename: Jeyoung
authors.initials: J
authors.lastname: Park
authors.forename: Eun Seong
authors.initials: ES
authors.lastname: Lee
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D004298:Dopamine / Q000737:chemistry; D004705:Endocytosis; D005260:Female; D015696:Gene Products, tat / Q000737:chemistry; D006046:Gold / Q000737:chemistry* / Q000378:metabolism* / Q000627:therapeutic use; D006801:Humans; D008024:Ligands; D053768:Metal Nanoparticles; D051379:Mice; D008958:Models, Molecular; D008968:Molecular Conformation; D010789:Phototherapy; D011092:Polyethylene Glycols / Q000737:chemistry; D014168:Transferrin / Q000737:chemistry
chemical_list: D015696:Gene Products, tat; D008024:Ligands; D014168:Transferrin; D011092:Polyethylene Glycols; D006046:Gold; D004298:Dopamine
keywords: Tat peptide; Y-shaped ligands; gold nanoparticles; photothermal ablation; transferrin