title: miR-320 enhances the sensitivity of human colon cancer cells to chemoradiotherapy in vitro by targeting FOXM1.
abstract: miR-320 expression level is found to be down-regulated in human colon cancer. To date, however, its underlying mechanisms in the chemo-resistance remain largely unknown. In this study, we demonstrated that ectopic expression of miR-320 led to inhibit HCT-116 cell proliferation, invasion and hypersensitivity to 5-Fu and Oxaliplatin. Also, knockdown of miR-320 reversed these effects in HT-29 cells. Furthermore, we identified an oncogene, FOXM1, as a direct target of miR-320. In addition, miR-320 could inactive the activity of Wnt/β-catenin pathway. Finally, we found that miR-320 and FOXM1 protein had a negative correlation in colon cancer tissues and adjacent normal tissues. These findings implied that miR-320-FOXM1 axis may overcome chemo-resistance of colon cancer cells and provide a new therapeutic target for the treatment of colon cancer.
authors.affiliation: Department of Oncology, The First Affiliated Hospital of Nanchang University, Nanchang 330006, PR China.
authors.forename: Lu-Ying
authors.initials: LY
authors.lastname: Wan
authors.affiliation: Department of Oncology, The First Affiliated Hospital of Nanchang University, Nanchang 330006, PR China.
authors.forename: Jun
authors.initials: J
authors.lastname: Deng
authors.affiliation: Department of Oncology, The First Affiliated Hospital of Nanchang University, Nanchang 330006, PR China.
authors.forename: Xiao-Jun
authors.initials: XJ
authors.lastname: Xiang
authors.affiliation: Department of Oncology, The First Affiliated Hospital of Nanchang University, Nanchang 330006, PR China.
authors.forename: Ling
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department of Oncology, The First Affiliated Hospital of Nanchang University, Nanchang 330006, PR China.
authors.forename: Feng
authors.initials: F
authors.lastname: Yu
authors.affiliation: Department of Oncology, The First Affiliated Hospital of Nanchang University, Nanchang 330006, PR China.
authors.forename: Jun
authors.initials: J
authors.lastname: Chen
authors.affiliation: Department of Oncology, The First Affiliated Hospital of Nanchang University, Nanchang 330006, PR China.
authors.forename: Zhe
authors.initials: Z
authors.lastname: Sun
authors.affiliation: Department of Oncology, The First Affiliated Hospital of Nanchang University, Nanchang 330006, PR China.
authors.forename: Miao
authors.initials: M
authors.lastname: Feng
authors.affiliation: Department of Oncology, The First Affiliated Hospital of Nanchang University, Nanchang 330006, PR China. Electronic address: jpxiong@medmail.com.cn.
authors.forename: Jian-Ping
authors.initials: JP
authors.lastname: Xiong
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics; D017209:Apoptosis / Q000187:drug effects / Q000235:genetics; D001483:Base Sequence; D002453:Cell Cycle / Q000187:drug effects / Q000235:genetics; D002465:Cell Movement / Q000187:drug effects / Q000235:genetics; D049109:Cell Proliferation / Q000187:drug effects / Q000235:genetics; D059248:Chemoradiotherapy; D003110:Colonic Neoplasms / Q000235:genetics* / Q000473:pathology / Q000628:therapy*; D005472:Fluorouracil / Q000494:pharmacology / Q000627:therapeutic use; D000072278:Forkhead Box Protein M1; D051858:Forkhead Transcription Factors / Q000235:genetics / Q000378:metabolism*; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D045325:HCT116 Cells; D019073:HT29 Cells; D006801:Humans; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D009361:Neoplasm Invasiveness; D009944:Organoplatinum Compounds / Q000494:pharmacology / Q000627:therapeutic use; D000077150:Oxaliplatin
chemical_list: D020413:3' Untranslated Regions; C107585:FOXM1 protein, human; D000072278:Forkhead Box Protein M1; D051858:Forkhead Transcription Factors; C572916:MIRN320 microRNA, human; D035683:MicroRNAs; D009944:Organoplatinum Compounds; D000077150:Oxaliplatin; D005472:Fluorouracil
keywords: Chemo-resistance; Colon cancer; FOXM1; Wnt; miR-320