title: Influence of muscarinic receptor modulators on interacerebroventricular injection of arachydonylcyclopropylamide induced antinociception in mice.
abstract: The interaction between antinociception induced by CB1 agonist and muscarinic receptor modulators has not been studied yet. In the present study, the effect of pilocarpine (a muscarinic agonist) and atropine (a muscarinic antagonist) on arachidonylcyclopropylamide (ACPA, a CB1 agonist) induced antinociception was studied in mice. In this study the antinociceptive effect of intracerebroventricular administration of ACPA (0.001-2 μg/mice) or intraperitoneal injection of pilocarpine (2.5-20mg/kg) or atropine (1 and 5mg/kg) were studied individually. Then the effect of co-administration of pilocarine (2.5mg/kg) or atropine (5mg/kg) and ACPA (0.001-2 μg/mice) were studied as well. ACPA and pilocarpine induced antinociception in mice but atropine did not. Pilocarpine potentiated but atropine antagonized the antinociceptive effect of ACPA. It is concluded that ACPA induced antinociception is influenced by muscarinic receptor modulators in mice.
authors.affiliation: Department of Physiology and Pharmacology, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran.
authors.forename: Mohammad R
authors.initials: MR
authors.lastname: Jafari
authors.affiliation: Department of Physiology and Pharmacology, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran.
authors.forename: Samaneh
authors.initials: S
authors.lastname: Onsori
authors.affiliation: Department of Medicine, McGill University, McIntyre Medical Bldg., Rm. 1112, 3655 Drummond St., Montreal QC, H3G 1Y6, Canada.
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Fekrmandi
authors.affiliation: Department of Physiology and Pharmacology, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran.
authors.forename: Pouria
authors.initials: P
authors.lastname: Tabrizian
authors.affiliation: Department of Physiology and Pharmacology, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran. Electronic address: alipourmohsen@yahoo.com.
authors.forename: Mohsen
authors.initials: M
authors.lastname: Alipour
authors.affiliation: Department of Pharmacology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Mohammad R
authors.initials: MR
authors.lastname: Zarrindast
mesh_terms: D018712:Analgesics, Non-Narcotic / Q000494:pharmacology*; D000818:Animals; D001095:Arachidonic Acids / Q000494:pharmacology*; D001285:Atropine / Q000494:pharmacology; D063386:Cannabinoid Receptor Agonists / Q000494:pharmacology; D002408:Catheters, Indwelling; D005557:Formaldehyde / Q000633:toxicity; D007276:Injections, Intraventricular; D008297:Male; D051379:Mice; D009043:Motor Activity / Q000187:drug effects; D018721:Muscarinic Agonists / Q000494:pharmacology*; D018727:Muscarinic Antagonists / Q000494:pharmacology*; D059226:Nociceptive Pain / Q000139:chemically induced / Q000188:drug therapy* / Q000503:physiopathology; D010147:Pain Measurement; D010862:Pilocarpine / Q000494:pharmacology; D043884:Receptor, Cannabinoid, CB1 / Q000819:agonists / Q000378:metabolism
chemical_list: D018712:Analgesics, Non-Narcotic; D001095:Arachidonic Acids; D063386:Cannabinoid Receptor Agonists; D018721:Muscarinic Agonists; D018727:Muscarinic Antagonists; D043884:Receptor, Cannabinoid, CB1; C119324:arachidonylcyclopropylamide; D010862:Pilocarpine; D005557:Formaldehyde; D001285:Atropine
keywords: ACPA; Antinociception; Atropine; Formalin test; Mice; Pilocarpine