title: SPRi determination of inter-peptide interaction by using 3D supramolecular co-assembly polyrotaxane film.
abstract: Accurate measurement of inter-peptide interactions is beneficial for in-depth understanding disease-related protein folding and peptide aggregation, and further for designing and selecting potential peptide drugs to the target antigen. Herein, we demonstrate a 3D polyrotaxane (PRX) surface for detecting peptides interactions by surface plasmon resonance imaging (SPRi). This surface is supramolecular self-assembly monolayer (SAM) structure fabricated by threading α-cyclodextrans (α-CD) through a linear polyethylene glycol (PEG) chain fixed on gold chip surface to form pseudopolyrotaxane, and further capping the pseudopolyrotaxane with bulky terminated group to form PRX film. The hydroxyl groups of α-CD can provide more active sites to increase molecules immobilization density, and PEG chain has unique protein non-fouling feature. We chose Alzheimer's disease marker β-amyloid 40 (Aβ40) as model peptide, and detected the interaction between it and its inhibitors KLVFFK6 by SPRi. As a striking result, the specific adsorption of KLVFFK6 solution at the concentration of 352μM on Aβ40-PRX was 700RU, whereas PEG SAM surface gave no significant binding. Interaction between other lower molecular weight peptides was detected via PRX surface, and the relatively weak interactions (KD=1.73×10(-4)M) between LPFFD (Mw=0.6kDa) and amylin20-29 (Mw=1.0kDa) are successfully detected.
authors.affiliation: National Center for Nanoscience and Technology, Beijing 100190, PR China.
authors.forename: Yanmei
authors.initials: Y
authors.lastname: Wang
authors.affiliation: National Center for Nanoscience and Technology, Beijing 100190, PR China; Department of Chemistry, Tsinghua University, Beijing 100084, PR China.
authors.forename: Chenxuan
authors.initials: C
authors.lastname: Wang
authors.affiliation: National Center for Nanoscience and Technology, Beijing 100190, PR China; Department of Chemistry, Tsinghua University, Beijing 100084, PR China.
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Cheng
authors.affiliation: National Center for Nanoscience and Technology, Beijing 100190, PR China.
authors.forename: Dongdong
authors.initials: D
authors.lastname: Zhang
authors.affiliation: National Center for Nanoscience and Technology, Beijing 100190, PR China.
authors.forename: Shaopeng
authors.initials: S
authors.lastname: Li
authors.affiliation: National Center for Nanoscience and Technology, Beijing 100190, PR China.
authors.forename: Lusheng
authors.initials: L
authors.lastname: Song
authors.affiliation: National Center for Nanoscience and Technology, Beijing 100190, PR China.
authors.forename: Wenfei
authors.initials: W
authors.lastname: Zhou
authors.affiliation: National Center for Nanoscience and Technology, Beijing 100190, PR China.
authors.forename: Mo
authors.initials: M
authors.lastname: Yang
authors.affiliation: National Center for Nanoscience and Technology, Beijing 100190, PR China.
authors.forename: Zhiyou
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Beihang University, Beijing 100191, PR China.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Zheng
authors.affiliation: National Center for Nanoscience and Technology, Beijing 100190, PR China.
authors.forename: Baohang
authors.initials: B
authors.lastname: Han
authors.affiliation: National Center for Nanoscience and Technology, Beijing 100190, PR China.
authors.forename: Chen
authors.initials: C
authors.lastname: Wang
authors.affiliation: National Center for Nanoscience and Technology, Beijing 100190, PR China.
authors.forename: Yanlian
authors.initials: Y
authors.lastname: Yang
authors.affiliation: National Center for Nanoscience and Technology, Beijing 100190, PR China.
authors.forename: Jinsong
authors.initials: J
authors.lastname: Zhu
mesh_terms: D000595:Amino Acid Sequence; D016229:Amyloid beta-Peptides / Q000032:analysis / Q000378:metabolism*; D003505:Cyclodextrins / Q000737:chemistry*; D004867:Equipment Design; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000032:analysis / Q000378:metabolism*; D010455:Peptides / Q000032:analysis / Q000378:metabolism*; D020442:Poloxamer / Q000737:chemistry*; D043862:Rotaxanes / Q000737:chemistry*; D020349:Surface Plasmon Resonance / Q000295:instrumentation*; D013499:Surface Properties
chemical_list: D016229:Amyloid beta-Peptides; D003505:Cyclodextrins; D010446:Peptide Fragments; D010455:Peptides; D043862:Rotaxanes; C078592:amyloid beta-protein (1-40); C475673:polyrotaxane; D020442:Poloxamer