title: Sensitive determination of fluoride in biological samples by gas chromatography-mass spectrometry after derivatization with 2-(bromomethyl)naphthalene.
abstract: A gas chromatography-mass spectrometric method was developed in this study in order to determine fluoride in plasma and urine after derivatization with 2-(bromomethyl)naphthalene. 2-Fluoronaphthalene was chosen as the internal standard. The derivatization of fluoride was performed in the biological sample and the best reaction conditions (10.0 mg mL(-1) of 2-(bromomethyl)naphthalene, 1.0 mg mL(-1) of 15-crown-5-ether as a phase transfer catalyst, pH of 7.0, reaction temperature of 70°C, and heating time of 70 min) were established. The organic derivative was extracted with dichloromethane and then measured by a gas chromatography-mass spectrometry. Under the established condition, the detection limits were 11 μg L(-1) and 7 μg L(-1) by using 0.2 mL of plasma or urine, respectively. The accuracy was in a range of 100.8-107.6%, and the precision of the assay was less than 4.3% in plasma or urine. Fluoride was detected in a concentration range of 0.12-0.53 mg L(-1) in six urine samples after intake of natural mineral water containing 0.7 mg L(-1) of fluoride.
authors.affiliation: Department of Environmental Science, Kongju National University, Kongju 314-701, Republic of Korea.
authors.forename: Sun-Myung
authors.initials: SM
authors.lastname: Kwon
authors.affiliation: Department of Environmental Education, Kongju National University, Kongju 314-701, Republic of Korea. Electronic address: hshin@kongju.ac.kr.
authors.forename: Ho-Sang
authors.initials: HS
authors.lastname: Shin
mesh_terms: D002138:Calibration; D005459:Fluorides / Q000097:blood* / Q000302:isolation & purification / Q000652:urine*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D057230:Limit of Detection; D009281:Naphthalenes / Q000737:chemistry
chemical_list: C541659:2,6-bis(bromomethyl)naphthalene; D009281:Naphthalenes; D005459:Fluorides
keywords: 2-(Bromomethyl)naphthalene; Derivatization; Fluoride; Gas chromatography–mass spectrometry