title: Brain region mapping using global metabolomics.
abstract: Historically, studies of brain metabolism have been based on targeted analyses of a limited number of metabolites. Here we present an untargeted mass spectrometry-based metabolomic strategy that has successfully uncovered differences in a broad array of metabolites across anatomical regions of the mouse brain. The NSG immunodeficient mouse model was chosen because of its ability to undergo humanization leading to numerous applications in oncology and infectious disease research. Metabolic phenotyping by hydrophilic interaction liquid chromatography and nanostructure imaging mass spectrometry revealed both water-soluble and lipid metabolite patterns across brain regions. Neurochemical differences in metabolic phenotypes were mainly defined by various phospholipids and several intriguing metabolites including carnosine, cholesterol sulfate, lipoamino acids, uric acid, and sialic acid, whose physiological roles in brain metabolism are poorly understood. This study helps define regional homeostasis for the normal mouse brain to give context to the reaction to pathological events.
authors.forename: Julijana
authors.initials: J
authors.lastname: Ivanisevic
authors.forename: Adrian A
authors.initials: AA
authors.lastname: Epstein
authors.forename: Michael E
authors.initials: ME
authors.lastname: Kurczy
authors.forename: Paul H
authors.initials: PH
authors.lastname: Benton
authors.forename: Winnie
authors.initials: W
authors.lastname: Uritboonthai
authors.forename: Howard S
authors.initials: HS
authors.lastname: Fox
authors.forename: Michael D
authors.initials: MD
authors.lastname: Boska
authors.forename: Howard E
authors.initials: HE
authors.lastname: Gendelman
authors.forename: Gary
authors.initials: G
authors.lastname: Siuzdak
mesh_terms: D000818:Animals; D001921:Brain / Q000033:anatomy & histology / Q000378:metabolism*; D001931:Brain Mapping; D002846:Chromatography, Affinity; D002851:Chromatography, High Pressure Liquid; D016000:Cluster Analysis; D057927:Hydrophobic and Hydrophilic Interactions; D053631:Interleukin Receptor Common gamma Subunit / Q000172:deficiency / Q000235:genetics / Q000378:metabolism; D055432:Metabolomics; D051379:Mice; D016688:Mice, Inbred NOD; D018345:Mice, Knockout; D016513:Mice, SCID; D049329:Nanostructures / Q000737:chemistry; D019906:Nuclear Magnetic Resonance, Biomolecular; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: C507942:Il2rg protein, mouse; D053631:Interleukin Receptor Common gamma Subunit