title: Kinetic of NO2 uptake by Phleum pratense pollen: chemical and allergenic implications.
abstract: Phleum pratense pollen was exposed to NO(2) in a reactor allowing a continuous analysis of NO(2) concentration by FTIR. The uptake coefficient of NO(2) on pollen was calculated postulating a first order kinetic reaction and a value of (1.1 ± 0.1) x 10(-7) was determined. NO(2) uptake was faster when the pollen water content was increased and when the pollen was pre-treated with ozone. The effect of NO(2) exposure on pollen allergic properties was investigated by quantifying Th2- and Th1-associated chemokines in a model of human dendritic cells. Cellular analysis clearly showed that cells exposed to fumigated pollen favored the production of chemokines known to promote Th2-cell responses. Altogether these data demonstrate that NO(2) uptake by pollen directly correlates with increased Th2 response in human cells,and are in favor of the involvement of NO(2) pollution in the increase of allergic diseases.
authors.affiliation: Physicochimie des Processus de Combustion et de l'Atmosphere, CNRS UMR 8522, Universite Lille 1, Universite Lille Nord de France, 59655 Villeneuve d'Ascq, France.
authors.forename: Guillaume
authors.initials: G
authors.lastname: Chassard
authors.forename: Marie
authors.initials: M
authors.lastname: Choël
authors.forename: Sylvie
authors.initials: S
authors.lastname: Gosselin
authors.forename: Han
authors.initials: H
authors.lastname: Vorng
authors.forename: Denis
authors.initials: D
authors.lastname: Petitprez
authors.forename: Youcef
authors.initials: Y
authors.lastname: Shahali
authors.forename: Anne
authors.initials: A
authors.lastname: Tsicopoulos
authors.forename: Nicolas
authors.initials: N
authors.lastname: Visez
mesh_terms: D000393:Air Pollutants / Q000032:analysis / Q000378:metabolism*; D000485:Allergens / Q000032:analysis; D006801:Humans; D009585:Nitrogen Dioxide / Q000032:analysis / Q000378:metabolism*; D010126:Ozone; D031784:Phleum / Q000378:metabolism*; D011058:Pollen
chemical_list: D000393:Air Pollutants; D000485:Allergens; D010126:Ozone; D009585:Nitrogen Dioxide