title: PXD101 significantly improves nuclear reprogramming and the in vitro developmental competence of porcine SCNT embryos.
abstract: In this study, we investigated the effects of the histone deacetylase inhibitor PXD101 (belinostat) on the preimplantation development of porcine somatic cell nuclear transfer (SCNT) embryos and their expression of the epigenetic markers histone H3 acetylated at lysine 9 (AcH3K9). We compared the in vitro developmental competence of SCNT embryos treated with various concentrations of PXD101 for 24h. Treatment with 0.5 μM PXD101 significantly increased the proportion of SCNT embryos that reached the blastocyst stage, in comparison to the control group (23.3% vs. 11.5%, P<0.05). We tested the in vitro developmental competence of SCNT embryos treated with 0.5 μM PXD101 for various amounts of times following activation. Treatment for 24h significantly improved the development of porcine SCNT embryos, with a significantly higher proportion of embryos reaching the blastocyst stage in comparison to the control group (25.7% vs. 10.6%, P<0.05). PXD101-treated SCNT embryos were transferred into two surrogate sows, one of whom became pregnant and four fetuses developed. PXD101 treatment significantly increased the fluorescence intensity of immunostaining for AcH3K9 in embryos at the pseudo-pronuclear and 2-cell stages. At these stages, the fluorescence intensities of immunostaining for AcH3K9 were significantly higher in PXD101-treated embryos than in control untreated embryos. In conclusion, this study demonstrates that PXD101 can significantly improve the in vitro and in vivo developmental competence of porcine SCNT embryos and can enhance their nuclear reprogramming.
authors.affiliation: Department of Animal Science, College of Agriculture, Yanbian University, Yanji 133000, China.
authors.forename: Jun-Xue
authors.initials: JX
authors.lastname: Jin
authors.affiliation: Department of Animal Science, College of Agriculture, Yanbian University, Yanji 133000, China.
authors.forename: Jin-Dan
authors.initials: JD
authors.lastname: Kang
authors.affiliation: Department of Animal Science, College of Agriculture, Yanbian University, Yanji 133000, China.
authors.forename: Suo
authors.initials: S
authors.lastname: Li
authors.affiliation: Department of Animal Science, College of Agriculture, Yanbian University, Yanji 133000, China.
authors.forename: Long
authors.initials: L
authors.lastname: Jin
authors.affiliation: Department of Animal Science, College of Agriculture, Yanbian University, Yanji 133000, China.
authors.forename: Hai-Ying
authors.initials: HY
authors.lastname: Zhu
authors.affiliation: Department of Animal Science, College of Agriculture, Yanbian University, Yanji 133000, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Guo
authors.affiliation: Department of Animal Science, College of Agriculture, Yanbian University, Yanji 133000, China.
authors.forename: Qing-Shan
authors.initials: QS
authors.lastname: Gao
authors.affiliation: Department of Animal Science, College of Agriculture, Yanbian University, Yanji 133000, China.
authors.forename: Chang-Guo
authors.initials: CG
authors.lastname: Yan
authors.affiliation: Department of Animal Science, College of Agriculture, Yanbian University, Yanji 133000, China. Electronic address: yinxj33@msn.com.
authors.forename: Xi-Jun
authors.initials: XJ
authors.lastname: Yin
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000166:cytology; D065150:Cellular Reprogramming / Q000187:drug effects* / Q000502:physiology*; D004622:Embryo, Mammalian / Q000187:drug effects*; D044127:Epigenesis, Genetic; D005260:Female; D005347:Fibroblasts / Q000378:metabolism; D056572:Histone Deacetylase Inhibitors / Q000494:pharmacology; D006657:Histones / Q000378:metabolism; D006877:Hydroxamic Acids / Q000494:pharmacology*; D053652:Nuclear Transfer Techniques; D009865:Oocytes / Q000166:cytology; D010053:Ovary / Q000378:metabolism; D011247:Pregnancy; D013449:Sulfonamides / Q000494:pharmacology*; D013552:Swine
chemical_list: D056572:Histone Deacetylase Inhibitors; D006657:Histones; D006877:Hydroxamic Acids; D013449:Sulfonamides; C487081:belinostat
keywords: Blastocyst; Histone deacetylase inhibitor (HDACi); In vitro development; PXD101; Somatic cell nuclear transfer (SCNT)