title: Glutathionyl-hydroquinone reductases from poplar are plastidial proteins that deglutathionylate both reduced and oxidized glutathionylated quinones.
abstract: Glutathionyl-hydroquinone reductases (GHRs) catalyze the deglutathionylation of quinones via a catalytic cysteine. The two GHR genes in the Populus trichocarpa genome, Pt-GHR1 and Pt-GHR2, are primarily expressed in reproductive organs. Both proteins are localized in plastids. More specifically, Pt-GHR2 localizes in nucleoids. At the structural level, Pt-GHR1 adopts a typical GHR fold, with a dimerization interface comparable to that of the bacterial and fungal GHR counterparts. Pt-GHR1 catalyzes the deglutathionylation of both reduced and oxidized glutathionylated quinones, but the enzyme is more catalytically efficient with the reduced forms.
authors.affiliation: Université de Lorraine, Interactions Arbres - Microorganismes, UMR1136, F-54500 Vandœuvre-lès-Nancy, France; INRA, Interactions Arbres - Microorganismes, UMR1136, F-54280 Champenoux, France.
authors.forename: Pierre-Alexandre
authors.initials: PA
authors.lastname: Lallement
authors.affiliation: Université de Lorraine, Interactions Arbres - Microorganismes, UMR1136, F-54500 Vandœuvre-lès-Nancy, France; INRA, Interactions Arbres - Microorganismes, UMR1136, F-54280 Champenoux, France.
authors.forename: Edgar
authors.initials: E
authors.lastname: Meux
authors.affiliation: Institut de Biologie Moléculaire des Plantes, CNRS-UPR 2357, 67084 Strasbourg, France.
authors.forename: José M
authors.initials: JM
authors.lastname: Gualberto
authors.affiliation: Laboratoire d'Etudes et de Recherches sur le Matériau Bois, EA 1093, Vandœuvre-lès-Nancy F-54506, France.
authors.forename: Stéphane
authors.initials: S
authors.lastname: Dumarcay
authors.affiliation: Université de Lorraine, CRM(2), Equipe BioMod, UMR 7036, Faculté des Sciences et Technologies, BP 70239, 54506 Vandœuvre-lès-Nancy, France; CNRS, CRM(2), Equipe BioMod, UMR 7036, Faculté des Sciences et Technologies, BP 70239, 54506 Vandœuvre-lès-Nancy, France.
authors.forename: Frédérique
authors.initials: F
authors.lastname: Favier
authors.affiliation: Université de Lorraine, CRM(2), Equipe BioMod, UMR 7036, Faculté des Sciences et Technologies, BP 70239, 54506 Vandœuvre-lès-Nancy, France; CNRS, CRM(2), Equipe BioMod, UMR 7036, Faculté des Sciences et Technologies, BP 70239, 54506 Vandœuvre-lès-Nancy, France.
authors.forename: Claude
authors.initials: C
authors.lastname: Didierjean
authors.affiliation: Institut Pasteur, Plate-Forme de Cristallographie, CNRS-UMR3528, 75724 Paris, France.
authors.forename: Frederick
authors.initials: F
authors.lastname: Saul
authors.affiliation: Institut Pasteur, Plate-Forme de Cristallographie, CNRS-UMR3528, 75724 Paris, France.
authors.forename: Ahmed
authors.initials: A
authors.lastname: Haouz
authors.affiliation: Université de Lorraine, Interactions Arbres - Microorganismes, UMR1136, F-54500 Vandœuvre-lès-Nancy, France; INRA, Interactions Arbres - Microorganismes, UMR1136, F-54280 Champenoux, France.
authors.forename: Mélanie
authors.initials: M
authors.lastname: Morel-Rouhier
authors.affiliation: Université de Lorraine, Interactions Arbres - Microorganismes, UMR1136, F-54500 Vandœuvre-lès-Nancy, France; INRA, Interactions Arbres - Microorganismes, UMR1136, F-54280 Champenoux, France.
authors.forename: Eric
authors.initials: E
authors.lastname: Gelhaye
authors.affiliation: Université de Lorraine, Interactions Arbres - Microorganismes, UMR1136, F-54500 Vandœuvre-lès-Nancy, France; INRA, Interactions Arbres - Microorganismes, UMR1136, F-54280 Champenoux, France.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Rouhier
authors.affiliation: Université de Lorraine, Interactions Arbres - Microorganismes, UMR1136, F-54500 Vandœuvre-lès-Nancy, France; INRA, Interactions Arbres - Microorganismes, UMR1136, F-54280 Champenoux, France. Electronic address: arnaud.hecker@univ-lorraine.fr.
authors.forename: Arnaud
authors.initials: A
authors.lastname: Hecker
mesh_terms: D020134:Catalytic Domain; D060365:Chloroplast Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D010084:Oxidation-Reduction; D010088:Oxidoreductases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D032107:Populus / Q000201:enzymology* / Q000235:genetics; D017510:Protein Folding; D055503:Protein Multimerization / Q000502:physiology*
chemical_list: D060365:Chloroplast Proteins; D010088:Oxidoreductases
keywords: Deglutathionylation; Glutathione transferase; Plastid; Poplar