title: Isoliquiritigenin attenuates oxidative hepatic damage induced by carbon tetrachloride with or without buthionine sulfoximine.
abstract: Glycyrrhizae radix (G. radix) has been demonstrated to have hepatoprotective properties. This study determined the therapeutic effects of isoliquiritigenin (isoLQ) in G. radix, against liver injury induced by CCl4 in rats. CCl4 (0.5 ml/kg/d, twice) or CCl4 plus buthionine sulfoximine exerted severe liver damage assessed by increased plasma levels of alanine aminotransferase and aspartate aminotransferase, in addition to hepatic degeneration and necrosis. These pathological changes were markedly protected by pretreatment with isoLQ (5, 20 mg/kg/d, p.o.) for 3 consecutive days. In addition, pretreatment with isoLQ inhibited CCl4-induced reduction of cytochrome P450 2E1 protein and mRNA expression as well as activity in the liver. Moreover, isoLQ pretreatment reversed the decrease in hepatic antioxidant capacity induced by CCl4 as well as suppressed expression of tumor necrosis factor-alpha and cyclooxigenase-2 in the liver. These results suggest that isoLQ has a protective effect against CCl4-induced liver damage through induction of antioxidant and anti-inflammatory activities.
authors.affiliation: College of Oriental Medicine, Daegu Haany University, Daegu 706-828, Republic of Korea; Department of Pharmacology, Mudanjiang Medical University, Heilongjiang 157011, China.
authors.forename: ZhengLin
authors.initials: Z
authors.lastname: Zhao
authors.affiliation: College of Oriental Medicine, Daegu Haany University, Daegu 706-828, Republic of Korea.
authors.forename: Sang Mi
authors.initials: SM
authors.lastname: Park
authors.affiliation: Department of Pharmacology, Mudanjiang Medical University, Heilongjiang 157011, China.
authors.forename: LiXin
authors.initials: L
authors.lastname: Guan
authors.affiliation: Department of Pharmacology, Mudanjiang Medical University, Heilongjiang 157011, China.
authors.forename: YiYan
authors.initials: Y
authors.lastname: Wu
authors.affiliation: College of Oriental Medicine, Daegu Haany University, Daegu 706-828, Republic of Korea.
authors.forename: Jong Rok
authors.initials: JR
authors.lastname: Lee
authors.affiliation: College of Oriental Medicine, Daegu Haany University, Daegu 706-828, Republic of Korea. Electronic address: sckim@dhu.ac.kr.
authors.forename: Sang Chan
authors.initials: SC
authors.lastname: Kim
authors.affiliation: College of Oriental Medicine, Daegu Haany University, Daegu 706-828, Republic of Korea. Electronic address: ywkim@dhu.ac.kr.
authors.forename: Young Woo
authors.initials: YW
authors.lastname: Kim
authors.affiliation: College of Oriental Medicine, Daegu Haany University, Daegu 706-828, Republic of Korea; Department of Pharmacology, Mudanjiang Medical University, Heilongjiang 157011, China. Electronic address: zhao_rongjie@yahoo.com.
authors.forename: RongJie
authors.initials: R
authors.lastname: Zhao
mesh_terms: D000410:Alanine Transaminase / Q000097:blood / Q000235:genetics; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood / Q000235:genetics; D019328:Buthionine Sulfoximine / Q000378:metabolism* / Q000633:toxicity; D002251:Carbon Tetrachloride / Q000378:metabolism* / Q000633:toxicity; D047188:Chalcones / Q000494:pharmacology* / Q000627:therapeutic use; D056486:Chemical and Drug Induced Liver Injury / Q000201:enzymology / Q000378:metabolism*; D019392:Cytochrome P-450 CYP2E1 / Q000378:metabolism*; D004791:Enzyme Inhibitors / Q000494:pharmacology* / Q000627:therapeutic use; D006651:Histocytochemistry; D008297:Male; D012333:RNA, Messenger / Q000737:chemistry / Q000235:genetics; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D047188:Chalcones; D004791:Enzyme Inhibitors; D012333:RNA, Messenger; D019328:Buthionine Sulfoximine; C040920:isoliquiritigenin; D002251:Carbon Tetrachloride; D019392:Cytochrome P-450 CYP2E1; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase
keywords: Carbon tetrachloride; Cytochrome P450 2E1; Isoliquiritigenin; Liver; Oxidative stress