title: Evaluation of bone tissue response to a sealer containing mineral trioxide aggregate.
abstract: BACKGROUND
  This study analyzed bone tissue reactions to MTA Fillapex (Ângelus Industria de Produtos Odontológicos Ltda, Londrina, Brazil) compared with an epoxy resin-based material in the femur of Wistar rats.
  
  
  METHODS
  Bone tissue reactions were evaluated in 15 animals after 7, 30, and 90 days (n = 5 per period). Three surgical cavities were prepared on the femur and filled with 0.2 mL MTA Fillapex, AH Plus (Dentsply DeTrey GmbH, Konstanz, Germany), or no sealer (negative control). By the end of each experimental period, 5 animals were randomly euthanized. The samples were histologically processed and analyzed using a light microscope. The presence of inflammatory cells, fibers, and hard tissue barrier formation was evaluated. Differences among the groups and between the 3 experimental periods were evaluated by using 2-way analysis of variance followed by the Bonferroni post hoc test (P ≤ .05).
  
  
  RESULTS
  MTA Fillapex scored significantly higher for neutrophils at 7 days than at 90. At 7 days, the same occurred when comparing MTA Fillapex with AH Plus. The presence of lymphocytes/plasmocytes significantly decreased over time in all groups. Macrophages, giant cells, eosinophils, and fiber condensation presented no differences among groups and periods. Within 90 days, all groups presented complete hard tissue barrier formation.
  
  
  CONCLUSIONS
  The presence of mineral trioxide aggregate in MTA Fillapex composition did not improve the bone tissue repair. The presence of sealers provided the re-establishment of the original bone tissue structure and the inflammatory response decreased over time, so they can be considered biocompatible.
authors.affiliation: Endodontic Division, Department of Conservative Dentistry, School of Dentistry, Federal University of Rio Grande do Sul, Porto Alegre, Rio Grande do Sol, Brazil.
authors.forename: Eloísa
authors.initials: E
authors.lastname: Assmann
authors.affiliation: Endodontic Division, Department of Conservative Dentistry, School of Dentistry, Federal University of Rio Grande do Sul, Porto Alegre, Rio Grande do Sol, Brazil. Electronic address: daibottcher@hotmail.com.
authors.forename: Daiana Elisabeth
authors.initials: DE
authors.lastname: Böttcher
authors.affiliation: Endodontic Division, Department of Conservative Dentistry, School of Dentistry, Federal University of Rio Grande do Sul, Porto Alegre, Rio Grande do Sol, Brazil.
authors.forename: Carolina Bender
authors.initials: CB
authors.lastname: Hoppe
authors.affiliation: Endodontic Division, Department of Conservative Dentistry, School of Dentistry, Federal University of Rio Grande do Sul, Porto Alegre, Rio Grande do Sol, Brazil.
authors.forename: Fabiana Soares
authors.initials: FS
authors.lastname: Grecca
authors.affiliation: Endodontic Division, Department of Conservative Dentistry, School of Dentistry, Federal University of Rio Grande do Sul, Porto Alegre, Rio Grande do Sol, Brazil.
authors.forename: Patrícia Maria Poli
authors.initials: PM
authors.lastname: Kopper
mesh_terms: D017607:Aluminum Compounds / Q000737:chemistry / Q000494:pharmacology*; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry / Q000494:pharmacology; D001842:Bone and Bones / Q000187:drug effects* / Q000473:pathology*; D017610:Calcium Compounds / Q000737:chemistry / Q000494:pharmacology*; D004338:Drug Combinations; D004853:Epoxy Resins / Q000737:chemistry / Q000494:pharmacology; D015726:Giant Cells / Q000187:drug effects / Q000473:pathology; D007249:Inflammation / Q000473:pathology; D008214:Lymphocytes / Q000187:drug effects / Q000473:pathology; D008264:Macrophages / Q000187:drug effects / Q000473:pathology; D008422:Materials Testing; D009504:Neutrophils / Q000187:drug effects / Q000473:pathology; D010087:Oxides / Q000737:chemistry / Q000494:pharmacology*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D012387:Root Canal Filling Materials / Q000737:chemistry / Q000494:pharmacology*; D017640:Silicates / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D017607:Aluminum Compounds; D001672:Biocompatible Materials; D017610:Calcium Compounds; D004338:Drug Combinations; D004853:Epoxy Resins; D010087:Oxides; D012387:Root Canal Filling Materials; D017640:Silicates; C086631:mineral trioxide aggregate
keywords: Biocompatibility; MTA Fillapex; endodontic sealers; endodontics; root canal filling