title: A Ga(3+)self-assembled fluorescent probe for ATP imaging in vivo.
abstract: Adenosine 5'-triphosphate (ATP) is a functional molecule associated with many important biological processes. Fluorescent detection methods for ATP with facile performance and high selectivity are in demand. One of the possible multi-membered arrays assembled between DHBO and Ga(3+) ions was conducted in aqueous solution, which can selectively recognize ATP with fluorescence enhancement from ADP, AMP and other structurally similar nucleoside triphosphates in vitro and in vivo. ATP facilitates the interaction between DHBO and Ga(3+) ions, resulting in the fluorescence increase. The detection limit for ATP was calculated to be 5.49×10(-7)M, which is much lower than that of intracellular concentrations (1-10mM). In addition, DHBO-Ga(3+) can be applied to detect ATP-relevant enzyme activity.
authors.affiliation: College of Science, Northwest A&F University, Yangling, Shaanxi 712100, PR China.
authors.forename: Liangliang
authors.initials: L
authors.lastname: Xiao
authors.affiliation: College of Science, Northwest A&F University, Yangling, Shaanxi 712100, PR China.
authors.forename: Shiguo
authors.initials: S
authors.lastname: Sun
authors.affiliation: College of Science, Northwest A&F University, Yangling, Shaanxi 712100, PR China.
authors.forename: Zhichao
authors.initials: Z
authors.lastname: Pei
authors.affiliation: College of Science, Northwest A&F University, Yangling, Shaanxi 712100, PR China.
authors.forename: Yuxin
authors.initials: Y
authors.lastname: Pei
authors.affiliation: Department of Chemistry & Maurice Morton Institute of Polymer Science, The University of Akron, Akron, OH 44325, USA.
authors.forename: Yi
authors.initials: Y
authors.lastname: Pang
authors.affiliation: College of Science, Northwest A&F University, Yangling, Shaanxi 712100, PR China. Electronic address: xuyq@nwsuaf.edu.cn.
authors.forename: Yongqian
authors.initials: Y
authors.lastname: Xu
mesh_terms: D000255:Adenosine Triphosphate / Q000032:analysis*; D001583:Benzoxazoles / Q000737:chemistry; D015374:Biosensing Techniques / Q000379:methods; D002412:Cations / Q000737:chemistry; D002460:Cell Line; D005456:Fluorescent Dyes / Q000737:chemistry*; D005708:Gallium / Q000737:chemistry*; D006801:Humans; D018613:Microscopy, Confocal / Q000379:methods; D061848:Optical Imaging / Q000379:methods*; D013050:Spectrometry, Fluorescence / Q000379:methods
chemical_list: D001583:Benzoxazoles; D002412:Cations; D005456:Fluorescent Dyes; D000255:Adenosine Triphosphate; D005708:Gallium
keywords: Cells imaging; Fluorescent sensors; Self-assembly