title: Polygonatum odoratum lectin induces apoptosis and autophagy via targeting EGFR-mediated Ras-Raf-MEK-ERK pathway in human MCF-7 breast cancer cells.
abstract: Polygonatum odoratum lectin (POL), a mannose-binding GNA-related lectin, has been reported to display remarkable anti-proliferative and apoptosis-inducing activities toward a variety of cancer cells; however, the precise molecular mechanisms by which POL induces cancer cell death are still elusive. In the current study, we found that POL could induce both apoptosis and autophagy in human MCF-7 breast cancer cells. Subsequently, we found that POL induced MCF-7 cell apoptosis via the mitochondrial pathway. Additionally, we also found that POL induces MCF-7 cell apoptosis via EGFR-mediated Ras-Raf-MEK-ERK pathway, suggesting that POL may be a potential EGFR inhibitor. Finally, we used proteomics analyses for exploring more possible POL-induced pathways with EGFR, Ras, Raf, MEK and ERK, some of which were consistent with our in silico network prediction. Taken together, these results demonstrate that POL induces MCF-7 cell apoptosis and autophagy via targeting EGFR-mediated Ras-Raf-MEK-ERK signaling pathway, which would provide a new clue for exploiting POL as a potential anti-neoplastic drug for future cancer therapy.
authors.affiliation: State Key Laboratory of Biotherapy and Collaborative Innovation Center of Biotherapy, Department of Gastrointestinal Surgery, West China Hospital, Sichuan University, Chengdu 610041, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Ouyang
authors.affiliation: State Key Laboratory of Biotherapy and Collaborative Innovation Center of Biotherapy, Department of Gastrointestinal Surgery, West China Hospital, Sichuan University, Chengdu 610041, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Analytical and Testing Center, Sichuan University, Chengdu 610064, China.
authors.forename: Xiao-yan
authors.initials: XY
authors.lastname: Wang
authors.affiliation: Department of Pediatrics, Key Laboratory of Obstetric and Gynecologic and Pediatric Diseases and Birth Defects of Ministry of Education, West China Second University Hospital, Sichuan University, Chengdu 610064, China.
authors.forename: Rui-feng
authors.initials: RF
authors.lastname: Lu
authors.affiliation: State Key Laboratory of Biotherapy and Collaborative Innovation Center of Biotherapy, Department of Gastrointestinal Surgery, West China Hospital, Sichuan University, Chengdu 610041, China.
authors.forename: Shou-yue
authors.initials: SY
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Biotherapy and Collaborative Innovation Center of Biotherapy, Department of Gastrointestinal Surgery, West China Hospital, Sichuan University, Chengdu 610041, China.
authors.forename: Mao
authors.initials: M
authors.lastname: Tian
authors.affiliation: State Key Laboratory of Biotherapy and Collaborative Innovation Center of Biotherapy, Department of Gastrointestinal Surgery, West China Hospital, Sichuan University, Chengdu 610041, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Xie
authors.affiliation: State Key Laboratory of Biotherapy and Collaborative Innovation Center of Biotherapy, Department of Gastrointestinal Surgery, West China Hospital, Sichuan University, Chengdu 610041, China. Electronic address: liubo2400@163.com.
authors.forename: Bo
authors.initials: B
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Biotherapy and Collaborative Innovation Center of Biotherapy, Department of Gastrointestinal Surgery, West China Hospital, Sichuan University, Chengdu 610041, China. Electronic address: heguscu@163.com.
authors.forename: Gu
authors.initials: G
authors.lastname: He
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D001343:Autophagy / Q000187:drug effects*; D066246:ErbB Receptors / Q000378:metabolism; D006801:Humans; D037102:Lectins / Q000494:pharmacology*; D020935:MAP Kinase Signaling System; D061986:MCF-7 Cells; D008928:Mitochondria / Q000378:metabolism; D031384:Polygonatum / Q000737:chemistry*
chemical_list: D037102:Lectins; C512478:EGFR protein, human; D066246:ErbB Receptors
keywords: Apoptosis; Autophagy; Epidermal growth factor receptor (EGFR); MCF-7 cell; Polygonatum odoratum lectin (POL)