title: [Role of myosins in depression of sensitivity of Helix neurons to acetylcholine in a cellular analog of habituation].
abstract: We investigated the involvement of cytoskeleton motor proteins, myosins, in the molecular mechanism of sensitivity depression to acetylcholine in Helix command neurons of defensive behavior in a cellular analog of habituation. There were analyzed the effects of several drugs disturbing myosin function: ML-7 and MLCK-IP-18--blockers of myosin light chain kinase, blebbistatin--an inhibitor of non-muscle myosin II, Y-27632--inhibitor of kinases ROCK-I and ROCK-II (activate mainly non-muscle myosin II) on the depression of acetylcholine-induced inward current. It was found that ML-7 and MLCK-IP- 18 weakened current depression; blebbistatin and Y-27632 did not change the depression. The results of experimental inhibitory analysis and mathematical modeling of the effects of inhibitors on the number of membrane-bound cholinergic receptors allow to suggest the involvement ofmyosins (excluding non-muscle myosin II) in the transports of acetylcholine receptors (endo- and exocytosis) that are responsible for sensitivity changes in neuron somatic membrane to acetylcholine in a cellular analog of habituation.
authors.forename: A S
authors.initials: AS
authors.lastname: Pivovarov
authors.forename: G B
authors.initials: GB
authors.lastname: Murzina
authors.forename: D A
authors.initials: DA
authors.lastname: Makhnovskiĭ
authors.forename: N A
authors.initials: NA
authors.lastname: Vasil'eva
authors.forename: M S
authors.initials: MS
authors.lastname: Tret'iakova
mesh_terms: D000109:Acetylcholine / Q000378:metabolism; D000577:Amides / Q000494:pharmacology; D000818:Animals; D001381:Azepines / Q000494:pharmacology; D001522:Behavior, Animal / Q000187:drug effects / Q000502:physiology*; D006372:Helix, Snails / Q000502:physiology; D006576:Heterocyclic Compounds, 4 or More Rings / Q000494:pharmacology; D009218:Myosins / Q000187:drug effects / Q000378:metabolism* / Q000502:physiology; D009281:Naphthalenes / Q000494:pharmacology; D009474:Neurons / Q000187:drug effects / Q000378:metabolism* / Q000502:physiology; D011725:Pyridines / Q000494:pharmacology; D011950:Receptors, Cholinergic / Q000187:drug effects / Q000378:metabolism
chemical_list: D000577:Amides; D001381:Azepines; D006576:Heterocyclic Compounds, 4 or More Rings; D009281:Naphthalenes; D011725:Pyridines; D011950:Receptors, Cholinergic; C070571:ML 7; C108830:Y 27632; C472645:blebbistatin; D009218:Myosins; D000109:Acetylcholine