title: Magnetic nanoparticles-serum proteins bioconjugates for binding of irinotecan.
abstract: The binding of irinotecan to serum proteins (hemoglobin, globulin and human serum albumin) was studied on the surface of epoxide modified superparamagnetic iron oxide nanoparticles (GPTS-SPIONs), which were synthesized by the coprecipitation of ferrous and ferric salts with NH4OH and then modified with [3-(2,3-epoxypropoxy)propyl] trimethoxy silane (GPTS) to obtain functional epoxide groups on the SPIONs' surface. Results were compared to find an alternative as drug carries system. Data showed that binding amount of human serum albumin (HSA), globulin (Glb) and hemoglobin (Hb) found to be as 44, 21.2 and 32.6 μg per 20 mg of GPTS modified SPIONs, respectively. The thermal behavior of the serum protein-Ir interaction on GPTS-SPIONs was also studied by using thermo gravimetric analysis (TGA) technique and then the kinetic parameters for the thermal decomposition were determined using Horowitz-Metzger method.
authors.affiliation: Department of Chemistry, Selcuk University, 42075 Konya, Turkey.
authors.forename: Ecem
authors.initials: E
authors.lastname: Tamyurek
authors.affiliation: Department of Chemistry, Selcuk University, 42075 Konya, Turkey.
authors.forename: Esra
authors.initials: E
authors.lastname: Maltas
authors.affiliation: Department of Chemistry, Selcuk University, 42075 Konya, Turkey.
authors.forename: Salih Zeki
authors.initials: SZ
authors.lastname: Bas
authors.affiliation: Department of Chemistry, Selcuk University, 42075 Konya, Turkey.
authors.forename: Mustafa
authors.initials: M
authors.lastname: Ozmen
authors.affiliation: Department of Chemistry, Selcuk University, 42075 Konya, Turkey. Electronic address: sayildiz@gmail.com.
authors.forename: Salih
authors.initials: S
authors.lastname: Yildiz
mesh_terms: D001798:Blood Proteins / Q000737:chemistry*; D002166:Camptothecin / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000737:chemistry; D004337:Drug Carriers / Q000737:chemistry; D016503:Drug Delivery Systems; D006801:Humans; D000077146:Irinotecan; D058185:Magnetite Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D011485:Protein Binding; D017550:Spectroscopy, Fourier Transform Infrared; D013816:Thermodynamics; D013818:Thermogravimetry
chemical_list: D001798:Blood Proteins; D004337:Drug Carriers; D058185:Magnetite Nanoparticles; D000077146:Irinotecan; D002166:Camptothecin
keywords: Fluorescence spectroscopy; Horowitz–Metzger method; Irinotecan; Magnetic nanoparticles; Protein