title: Purification of Schwann cells.
abstract: This article introduces methods for the acute purification and primary culture of Schwann cells from the mouse sciatic nerve. Immunopanning can be used to isolate Schwann cells from intact nerves during early postnatal development as well as to purify Schwann cells from adult nerves following sciatic nerve injury. These methods facilitate the exploration of mouse Schwann cell biology in the healthy and injured peripheral nerve.
authors.affiliation: Stanford University School of Medicine, Department of Neurobiology, Stanford, California 94305.
authors.forename: Amanda Brosius
authors.initials: AB
authors.lastname: Lutz
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D002469:Cell Separation / Q000379:methods*; D002478:Cells, Cultured; D051379:Mice; D012583:Schwann Cells / Q000166:cytology*