title: Early growth promotion and leaf level physiology changes in Burkholderia phytofirmans strain PsJN inoculated switchgrass.
abstract: Switchgrass (SG) is one of the most promising next generation biofuel crops in North America. Inoculation with bacterial endophytes has improved growth of several plant species. Our study demonstrated that Burkholderia phytofirmans strain PsJN, a well-studied plant growth promoting rhizo-bacterium (PGPR) significantly increased both aboveground and belowground biomass (DW) and promoted elongation of root, stem and leaf within 17 days following inoculation. Furthermore, the enhanced root growth in PsJN inoculated plants lagged behind the shoot response, resulting in greater allocation to aboveground growth (p = 0.0041). Lower specific root length (SRL, p = 0.0158) and higher specific leaf weight (SLW, p = 0.0029) were also observed in PsJN inoculated seedlings, indicating changes in development. Photosynthetic rates (Ps) were also significantly higher in PsJN inoculated seedlings after 17 days (54%, p = 0.0016), and this occurred initially without increases in stomatal conductance resulting in significantly greater water use efficiency (WUE, 37.7%, p = 0.0467) and lower non-stomatal limitation (LNS, 29.6%, p = 0.0222). These rapid changes in leaf level physiology are at least partially responsible for the growth enhancement due to PsJN.
authors.affiliation: Department of Forest Resources and Environmental Conservation, Virginia Tech, Blacksburg, VA 24061, USA. Electronic address: wangbx@vt.edu.
authors.forename: Bingxue
authors.initials: B
authors.lastname: Wang
authors.affiliation: Center for Sustainable and Renewable Resources, Institute of Advanced Learning and Research, Danville, VA 24540, USA. Electronic address: Chuansheng.Mei@ialr.org.
authors.forename: Chuansheng
authors.initials: C
authors.lastname: Mei
authors.affiliation: Department of Forest Resources and Environmental Conservation, Virginia Tech, Blacksburg, VA 24061, USA. Electronic address: Jseiler@vt.edu.
authors.forename: John R
authors.initials: JR
authors.lastname: Seiler
mesh_terms: D018533:Biomass; D019117:Burkholderia / Q000502:physiology*; D060026:Endophytes / Q000502:physiology*; D054884:Host-Pathogen Interactions; D008897:Panicum / Q000254:growth & development / Q000378:metabolism / Q000382:microbiology*; D010788:Photosynthesis / Q000502:physiology; D018515:Plant Leaves / Q000254:growth & development / Q000378:metabolism / Q000382:microbiology*; D018517:Plant Roots / Q000254:growth & development / Q000378:metabolism / Q000382:microbiology; D018547:Plant Stems / Q000254:growth & development / Q000378:metabolism / Q000382:microbiology; D054046:Plant Stomata / Q000502:physiology; D036226:Seedlings / Q000254:growth & development / Q000378:metabolism / Q000382:microbiology; D013997:Time Factors
keywords: Enhanced growth; Photosynthesis; Plant growth promoting rhizobacteria (PGPR); PsJN; Switchgrass; Water use efficiency