title: Involvement of soil bacteria in ABO blood mistyping.
abstract: The current study investigated whether ABO blood mistyping of human biological samples is induced by soil bacteria. A total of 380 bacterial strains were isolated from 50 discrete soil samples using human blood agar, and glycosidase activity evaluated for all strains using 4-nitropheny glycosides (4-nitrophenyl n-acetyl-α-D-galactosaminide, 4-nitrophenyl-α-D-galactopyranoside, 4-nitrophenyl-α-L-fucopyranoside) as substrates. Thirteen strains possessed α-galactosidase activity, and 16S rRNA sequence analysis revealed a close relatedness to the genus Bacillus. An indirect competitive enzyme-linked immunosorbent assay confirmed seven strains exhibited type B antigen degradation activity. These results demonstrated that 1.8% of the bacteria isolated from soil, were Bacillus sp., possessed galactosidase activity, and had the potential to cause ABO blood mistyping.
authors.affiliation: Science of Biological Resources, United Graduate School of Agricultural Sciences, Gifu University, Gifu 501-1193, Japan; Forensic Science Laboratory, Gifu Prefectural Police Headquarters, Gifu 500-8501, Japan. Electronic address: naokiutd_1977@ybb.ne.jp.
authors.forename: Naoki
authors.initials: N
authors.lastname: Takada
authors.affiliation: Forensic Science Laboratory, Gifu Prefectural Police Headquarters, Gifu 500-8501, Japan.
authors.forename: Chikahiro
authors.initials: C
authors.lastname: Mori
authors.affiliation: Forensic Science Laboratory, Gifu Prefectural Police Headquarters, Gifu 500-8501, Japan.
authors.forename: Rie
authors.initials: R
authors.lastname: Takai
authors.affiliation: Forensic Science Laboratory, Gifu Prefectural Police Headquarters, Gifu 500-8501, Japan.
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Takayama
authors.affiliation: Forensic Science Laboratory, Gifu Prefectural Police Headquarters, Gifu 500-8501, Japan.
authors.forename: Yoshihisa
authors.initials: Y
authors.lastname: Watanabe
authors.affiliation: Faculty of Applied Biological Sciences, Gifu University, Gifu 501-1193, Japan.
authors.forename: Kohei
authors.initials: K
authors.lastname: Nakamura
authors.affiliation: Faculty of Applied Biological Sciences, Gifu University, Gifu 501-1193, Japan.
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Takamizawa
mesh_terms: D000017:ABO Blood-Group System / Q000032:analysis*; D001407:Bacillus / Q000201:enzymology*; D001789:Blood Group Antigens / Q000378:metabolism; D001788:Blood Grouping and Crossmatching; D004797:Enzyme-Linked Immunosorbent Assay; D006026:Glycoside Hydrolases / Q000378:metabolism; D006801:Humans; D012988:Soil Microbiology
chemical_list: D000017:ABO Blood-Group System; D001789:Blood Group Antigens; D006026:Glycoside Hydrolases
keywords: ABO antigen; ABO blood mistyping