title: Antibacterial and cytotoxic potential of silver nanoparticles synthesized using latex of Calotropis gigantea L.
abstract: The present study aimed to synthesis silver nanoparticles (AgNPs) in a greener route using aqueous latex extract of Calotropis gigantea L. toward biomedical applications. Initially, synthesis of AgNPs was confirmed through UV-Vis spectroscopy which shows the surface plasmonic resonance peak (SPR) at 420 nm. Fourier transform infrared spectroscopy (FTIR) analysis provides clear evidence that protein fractions present in the latex extract act as reducing and stabilizing bio agents. Energy dispersive X-ray (EDAX) spectroscopy confirms the presence of silver as a major constituent element. X-ray diffractograms displays that the synthesized AgNPs were biphasic crystalline nature. Electron microscopic studies such as Field emission scanning electron microscopic (Fe-SEM) and Transmission electron microscope (TEM) reveals that synthesized AgNPs are spherical in shape with the size range between 5 and 30 nm. Further, crude latex aqueous extract and synthesized AgNPs were evaluated against different bacterial pathogens such as Bacillus cereus, Enterococci sp, Shigella sp, Pseudomonas aeruginosa, Klebsiella pneumonia, Staphylococcus aureus and Escherichia coli. Compared to the crude latex aqueous extract, biosynthesized AgNPs exhibits a remarkable antimicrobial activity. Likewise in vitro anticancer study manifests the cytotoxicity value of synthesized AgNPs against tested HeLa cells. The output of this study clearly suggesting that biosynthesized AgNPs using latex of C. gigantea can be used as promising nanomaterial for therapeutic application in context with nanodrug formulation.
authors.affiliation: Department of Biotechnology and Genetic Engineering, Bharathidasan University, Tiruchirappalli 620024, Tamil Nadu, India.
authors.forename: Chandrasekaran
authors.initials: C
authors.lastname: Rajkuberan
authors.affiliation: Department of Biotechnology and Genetic Engineering, Bharathidasan University, Tiruchirappalli 620024, Tamil Nadu, India.
authors.forename: Kannaiah
authors.initials: K
authors.lastname: Sudha
authors.affiliation: Department of Biotechnology and Genetic Engineering, Bharathidasan University, Tiruchirappalli 620024, Tamil Nadu, India.
authors.forename: Gnanasekar
authors.initials: G
authors.lastname: Sathishkumar
authors.affiliation: Department of Biotechnology and Genetic Engineering, Bharathidasan University, Tiruchirappalli 620024, Tamil Nadu, India. Electronic address: sivaramakrishnan123@yahoo.com.
authors.forename: Sivaperumal
authors.initials: S
authors.lastname: Sivaramakrishnan
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects; D031107:Calotropis / Q000737:chemistry*; D016923:Cell Death / Q000187:drug effects; D006367:HeLa Cells; D006801:Humans; D007840:Latex / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D008826:Microbial Sensitivity Tests; D012834:Silver / Q000494:pharmacology*; D013056:Spectrophotometry, Ultraviolet
chemical_list: D000900:Anti-Bacterial Agents; D007840:Latex; D012834:Silver
keywords: Calotropis gigantea L.; Cytotoxicity; SPR; Silver nanoparticles; XRD