title: The effect of moonlighting proteins on the adhesion and aggregation ability of Lactobacillus helveticus.
abstract: The goal of this study was to identify moonlighting proteins in Lactobacillus helveticus that play an important role in adhesion and aggregation. The label-free method was used for identification and analysis of expression of cellular proteins. The analysis revealed the presence of eight moonlighting proteins in the cell envelope of Lb. helveticus. The tested strains mainly differed with respect to the presence of S-layer proteins and the level of expression of moonlighting proteins in Lb. helveticus strain T159. These surface proteins give the cell a hydrophobic character and play a role in specific interactions with intestinal epithelium cells and with other bacteria. In Lb. helveticus T159, the S-layer associated with moonlighting proteins could act as adherence factors, which was evidenced by the high capability of adhesion, auto- and coaggregation. The hydrophobicity, adhesion and aggregation abilities provide biological activities in food products and they are regarded as an important criterion for probiotic selection.
authors.affiliation: Department of Biotechnology, Human Nutrition and Food Commodity Science, University of Life Sciences in Lublin, Skromna 8, 20-704 Lublin, Poland; Institute of Agrophysics, Polish Academy of Sciences, Doświadczalna 4, 20-290 Lublin, Poland. Electronic address: awasko1@tlen.pl.
authors.forename: Adam
authors.initials: A
authors.lastname: Waśko
authors.affiliation: Department of Biotechnology, Human Nutrition and Food Commodity Science, University of Life Sciences in Lublin, Skromna 8, 20-704 Lublin, Poland.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Polak-Berecka
authors.affiliation: Department of Virology and Immunology, Institute of Microbiology and Biotechnology, Maria Curie-Skłodowska University, Poland.
authors.forename: Roman
authors.initials: R
authors.lastname: Paduch
authors.affiliation: Laboratory of Medicinal Chemistry and Neuroengineering, Medical University of Lublin, Poland.
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Jóźwiak
keywords: Adhesion; Label-free method; Lactobacillus helveticus; Moonlighting proteins; Surface properties