title: Fatty acid profiles from the plasma membrane and detergent resistant membranes of two plant species.
abstract: It is essential to establish the composition of the plant plasma membrane in order to understand its organization and behavior under continually changing environments. Knowledge of the lipid phase, in particular the fatty acid (FA) complex repertoire, is important since FAs determine many of the physical-chemical membrane properties. FAs are constituents of the membrane glycerolipid and sphingolipid backbones and can also be linked to some sterols. In addition, FAs are components of complex lipids that can constitute membrane micro-domains, and the use of detergent-resistant membranes is a common approach to study their composition. The diversity and cellular allocation of the membrane lipids containing FAs are very diverse and the approaches to analyze them provide only general information. In this work, a detailed FA analysis was performed using highly purified plasma membranes from bean leaves and germinating maize embryos and their respective detergent-resistant membrane preparations. The analyses showed the presence of a significant amount of very long chain FAs (containing 28C, 30C and 32C), in both plasma membrane preparations from bean and maize, that have not been previously reported. Herein is demonstrated that a significant enrichment of very long chain saturated FAs and saturated FAs can occur in detergent-resistant membrane preparations, as compared to the plasma membranes from both plant species. Considering that a thorough analysis of FAs is rarely performed in purified plasma membranes and detergent-resistant membranes, this work provides qualitative and quantitative evidence on the contributions of the length and saturation of FAs to the organization of the plant plasma membrane and detergent-resistant membranes.
authors.affiliation: Dpto. de Bioquímica, Edif. E, Facultad de Química, UNAM. Ciudad Universitaria, Coyoacán, 04510 Mexico, D.F., Mexico.
authors.forename: Laura
authors.initials: L
authors.lastname: Carmona-Salazar
authors.affiliation: Dpto. de Biomedicina Cardiovascular, Instituto Nacional de Cardiología Ignacio Chávez, Juan Badiano No. 1, Tlalpan, 14080 México, D.F., Mexico.
authors.forename: Mohammed
authors.initials: M
authors.lastname: El Hafidi
authors.affiliation: Laboratorio de Bioquímica, Instituto Nacional de Medicina Genómica, Periférico Sur No. Col. Arenal Tepepan, Tlalpan, México, D.F., Mexico.
authors.forename: Nora
authors.initials: N
authors.lastname: Gutiérrez-Nájera
authors.affiliation: Dpto. de Bioquímica, Edif. E, Facultad de Química, UNAM. Ciudad Universitaria, Coyoacán, 04510 Mexico, D.F., Mexico.
authors.forename: Liliana
authors.initials: L
authors.lastname: Noyola-Martínez
authors.affiliation: Dpto. de Bioquímica, Edif. E, Facultad de Química, UNAM. Ciudad Universitaria, Coyoacán, 04510 Mexico, D.F., Mexico.
authors.forename: Ariadna
authors.initials: A
authors.lastname: González-Solís
authors.affiliation: Dpto. de Bioquímica, Edif. E, Facultad de Química, UNAM. Ciudad Universitaria, Coyoacán, 04510 Mexico, D.F., Mexico. Electronic address: gavilan@unam.mx.
authors.forename: Marina
authors.initials: M
authors.lastname: Gavilanes-Ruíz
mesh_terms: D002462:Cell Membrane / Q000737:chemistry*; D003902:Detergents; D005227:Fatty Acids / Q000737:chemistry*; D027805:Phaseolus / Q000737:chemistry*; D003313:Zea mays / Q000737:chemistry*
chemical_list: D003902:Detergents; D005227:Fatty Acids
keywords: Common bean; Detergent-resistant membranes; Fam. Fabaceae; Fam. Poaceae; Fatty acids; Maize; Membrane lipids; Phaseolus vulgaris; Plant plasma membranes; Zea mays