title: Mitochondrial DNA control region sequences study in Saraiki population from Pakistan.
abstract: The analysis of mitochondrial DNA (mtDNA) control region was carried in 85 unrelated Sariki individuals living in the different provinces of Pakistan. DNA was extracted from blood preserved in EDTA vacutainers. Hypervariable regions (HV1, HV2 & HV3) were PCR amplified and sequenced. Sequencing results were aligned and compared with revised Cambridge reference sequence (rCRS). The sequencing results showed presence of total 63 different haplotypes, 58 of them are unique and 05 are common haplotypes shared by more than one individual. The most common haplotype observed was (W6) with a frequency 12.9% of population sample. The Saraiki population was detected with genetic diversity (0.9570) and power of discrimination (0.9458). This study will be beneficial for forensic casework.
authors.affiliation: Department of Zoology, University of the Punjab, Lahore, Pakistan. Electronic address: skndrhayat93@gmail.com.
authors.forename: Sikandar
authors.initials: S
authors.lastname: Hayat
authors.affiliation: Department of Zoology, University of the Punjab, Lahore, Pakistan.
authors.forename: Tanveer
authors.initials: T
authors.lastname: Akhtar
authors.affiliation: Department of Zoology, University of the Punjab, Lahore, Pakistan.
authors.forename: Muhammad Hassan
authors.initials: MH
authors.lastname: Siddiqi
authors.affiliation: Department of Forensic Medicine, University of Health Sciences, Lahore, Pakistan.
authors.forename: Allah
authors.initials: A
authors.lastname: Rakha
authors.affiliation: Department of Zoology, University of the Punjab, Lahore, Pakistan.
authors.forename: Naeem
authors.initials: N
authors.lastname: Haider
authors.affiliation: Department of Zoology, University of the Punjab, Lahore, Pakistan.
authors.forename: Muhammad
authors.initials: M
authors.lastname: Tayyab
authors.affiliation: Department of Zoology, University of the Punjab, Lahore, Pakistan.
authors.forename: Ghazanfar
authors.initials: G
authors.lastname: Abbas
authors.affiliation: Department of Forensic Science, Institute of Molecular Biology & Biotechnology, University of Lahore, Pakistan.
authors.forename: Azam
authors.initials: A
authors.lastname: Ali
authors.affiliation: Department of Zoology, University of the Punjab, Lahore, Pakistan.
authors.forename: Syed Yassir Abbas
authors.initials: SY
authors.lastname: Bokhari
authors.affiliation: Department of Biosciences, COMSATS Institute of Information Technology, Sahiwal, Pakistan.
authors.forename: Muhammad Akram
authors.initials: MA
authors.lastname: Tariq
authors.affiliation: Department of Zoology, University of the Punjab, Lahore, Pakistan.
authors.forename: Fazle Majid
authors.initials: FM
authors.lastname: Khan
mesh_terms: D044466:Asian People / Q000235:genetics*; D004272:DNA, Mitochondrial / Q000235:genetics*; D006239:Haplotypes; D006801:Humans; D010154:Pakistan
chemical_list: D004272:DNA, Mitochondrial
keywords: Haplotype; Hypervariable regions; Mitochondrial DNA; Pakistan; Saraiki population