title: Rational design of medium supplementation strategy for improved influenza viruses production based on analyzing nutritional requirements of MDCK Cells.
abstract: Influenza vaccine production using cell culture technology has become popular nowadays. However, to meet the ever increasing demand of influenza vaccine, it is prerequisite to improve the yield of influenza virus in cells. To achieve this, in the present study, the nutritional requirements of MDCK cells in the virus production process were analyzed and a nutrient-feeding strategy was developed accordingly. Based on the consumption rates and corresponding concentration optimization, glucose and fast metabolized amino acids were supplemented into the maintaining medium at the time of infection. Compared with the non-supplemented culture, the average cell specific death rate during 0-48 h post-infection was 0.013 h(-1), which was 40.91% lower in the nutrient-supplemented culture. Total virus titer, HA antigen protein concentration and cell-specific virus yield were (1.88±0.23)×10(3) HA units/50μL, 11.70±0.22 μg/mL and (10.06±1.16)×10(3) virions/cell, respectively, which were 84.04±22.50%, 31.46±2.87% and 86.64±25.81% higher than those in the control, respectively. These data showed that the appropriate supplementation of nutrients during virus production process could reduce cell death, and improve cell-specific virus yield and total influenza virus output. This study laid foundation for the development of cell culture technology for influenza vaccine production.
authors.affiliation: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China.
authors.forename: Ding
authors.initials: D
authors.lastname: Huang
authors.affiliation: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China.
authors.forename: Kang
authors.initials: K
authors.lastname: Xia-Hou
authors.affiliation: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China. Electronic address: xupingliu@ecust.edu.cn.
authors.forename: Xu-Ping
authors.initials: XP
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Zhao
authors.affiliation: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China.
authors.forename: Li
authors.initials: L
authors.lastname: Fan
authors.affiliation: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China.
authors.forename: Zhaoyang
authors.initials: Z
authors.lastname: Ye
authors.affiliation: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China. Electronic address: wstan@ecust.edu.cn.
authors.forename: Wen-Song
authors.initials: WS
authors.lastname: Tan
authors.affiliation: Shanghai Institute of Biological Products Co., Ltd., Shanghai 200052, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Luo
authors.affiliation: Shanghai Institute of Biological Products Co., Ltd., Shanghai 200052, China.
authors.forename: Ze
authors.initials: Z
authors.lastname: Chen
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods; D003470:Culture Media / Q000737:chemistry*; D004285:Dogs; D007252:Influenza Vaccines / Q000302:isolation & purification*; D061985:Madin Darby Canine Kidney Cells; D009975:Orthomyxoviridae / Q000254:growth & development* / Q000302:isolation & purification*; D013678:Technology, Pharmaceutical / Q000379:methods*; D019562:Viral Load
chemical_list: D003470:Culture Media; D007252:Influenza Vaccines
keywords: Influenza virus; MDCK cells; Metabolism; Nutritional requirements