title: Impact of carbon and nitrogen feeding strategy on high production of biomass and docosahexaenoic acid (DHA) by Schizochytrium sp. LU310.
abstract: A new isolated Schizochytrium sp. LU310 from the mangrove forest of Wenzhou, China, was found as a high producing microalga of docosahexaenoic acid (DHA). In this study, the significant improvements for DHA fermentation by the batch mode in the baffled flasks (i.e. higher oxygen supply) were achieved. By applied the nitrogen-feeding strategy in 1000 mL baffled flasks, the biomass, DHA concentration and DHA productivity were increased by 110.4%, 117.9% and 110.4%, respectively. Moreover, DHA concentration of 21.06 g/L was obtained by feeding 15 g/L of glucose intermittently, which was an increase of 41.25% over that of the batch mode. Finally, an innovative strategy was carried out by intermittent feeding carbon and simultaneously feeding nitrogen. The maximum DHA concentration and DHA productivity in the fed-batch cultivation reached to 24.74 g/L and 241.5 mg/L/h, respectively.
authors.affiliation: Department of Chemical and Biochemical Engineering, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, PR China.
authors.forename: Xueping
authors.initials: X
authors.lastname: Ling
authors.affiliation: Department of Chemical and Biochemical Engineering, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, PR China.
authors.forename: Jing
authors.initials: J
authors.lastname: Guo
authors.affiliation: Department of Chemical and Biochemical Engineering, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, PR China.
authors.forename: Xiaoting
authors.initials: X
authors.lastname: Liu
authors.affiliation: Department of Chemical and Biochemical Engineering, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, PR China.
authors.forename: Xia
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Chemical and Biochemical Engineering, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, PR China.
authors.forename: Nan
authors.initials: N
authors.lastname: Wang
authors.affiliation: Department of Chemical and Biochemical Engineering, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, PR China.
authors.forename: Yinghua
authors.initials: Y
authors.lastname: Lu
authors.affiliation: Department of Chemical and Biochemical Engineering, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, PR China; Department of Chemical Engineering, National Cheng Kung University, Tainan 70101, Taiwan. Electronic address: yswu@mail.ncku.edu.tw.
authors.forename: I-Son
authors.initials: IS
authors.lastname: Ng
mesh_terms: D001483:Base Sequence; D061249:Batch Cell Culture Techniques; D018533:Biomass; D019149:Bioreactors / Q000382:microbiology; D002244:Carbon / Q000494:pharmacology*; D004281:Docosahexaenoic Acids / Q000096:biosynthesis*; D005227:Fatty Acids / Q000378:metabolism; D005285:Fermentation / Q000187:drug effects; D009584:Nitrogen / Q000494:pharmacology*; D010100:Oxygen / Q000494:pharmacology; D010802:Phylogeny; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D012970:Sodium Glutamate / Q000494:pharmacology; D058009:Stramenopiles / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D013997:Time Factors
chemical_list: D005227:Fatty Acids; D012337:RNA, Ribosomal, 18S; D004281:Docosahexaenoic Acids; D002244:Carbon; D009584:Nitrogen; D010100:Oxygen; D012970:Sodium Glutamate
keywords: Baffled flasks; Carbon feeding; Docosahexaenoic acid (DHA); Nitrogen feeding; Schizochytrium sp.