title: Phosphorus metabolism and population dynamics in a biological phosphate-removal system with simultaneous anaerobic phosphate stripping.
abstract: In this study, the metabolism of phosphorus and changes in population dynamics were investigated via simultaneous chemical stripping in sidestream in an acetate-fed sequencing batch reactor. The synthesized intracellular polyphosphate (poly-P) by polyphosphate-accumulating organisms (PAOs) gradually decreased when the biomass was subjected to 83 d of P stripping. Initially, the P removal efficiency of the system improved from 94.3% to 96.9%. Thereafter, a relatively high level of P in effluent was observed, during which time the stoichiometric ratios of Prelease/HAcuptake decreased, Glycogendegraded/HAcuptake and poly-β-hydroxyvalerate/PHA increased. The results revealed that a metabolic shift from polyphosphate-accumulating metabolism to glycogen-accumulating metabolism. Correspondingly, PAOs declined to less than 1% of the population, glycogen-accumulating organisms proliferated to almost 20% instead. The results of PCR–DGGE also indicated that the microbial community structure considerably changed in response to the gradually decreasing poly-P content. These findings imply that intracellular poly-P level is important for the stable of P removal system. Furthermore, it suggests that it is not a stable and effective way for P recycling from anaerobic stage of the biological P removal system in sidestream.
authors.affiliation: School of Environmental and Municipal Engineering, Xi’an University of Architecture and Technology, Xi’an, Shaanxi 710055, PR China.
authors.forename: Jing-hua
authors.initials: JH
authors.lastname: Lv
authors.forename: Lin-jiang
authors.initials: LJ
authors.lastname: Yuan
authors.forename: Xi
authors.initials: X
authors.lastname: Chen
authors.forename: Lun
authors.initials: L
authors.lastname: Liu
authors.forename: Da-cheng
authors.initials: DC
authors.lastname: Luo
mesh_terms: D000332:Aerobiosis; D000693:Anaerobiosis; D001419:Bacteria / Q000378:metabolism*; D018533:Biomass; D019149:Bioreactors; D058645:Denaturing Gradient Gel Electrophoresis; D017404:In Situ Hybridization, Fluorescence; D010758:Phosphorus / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D011122:Polyphosphates / Q000378:metabolism*; D011157:Population Dynamics; D014865:Waste Disposal, Fluid / Q000379:methods*
chemical_list: D011122:Polyphosphates; D010758:Phosphorus