title: A YadA-like autotransporter, Hag1 in Veillonella atypica is a multivalent hemagglutinin involved in adherence to oral streptococci, Porphyromonas gingivalis, and human oral buccal cells.
abstract: Dental biofilm development is a sequential process, and adherence between microbes and the salivary pellicle (adhesion) as well as among different microbes (co-adhesion or coaggregation) plays a critical role in building a biofilm community. The Veillonella species are among the most predominant species in the oral cavity and coaggregate with many initial, early, middle, and late colonizers. Similar to oral fusobacteria, they are also considered bridging species in biofilm development. However, the mechanism of this ability has yet to be reported, due to the previous lack of a genetic transformation system in the entire genus. In this study, we used our recently discovered transformable Veillonella strain, Veillonella atypica OK5, to probe the mechanism of coaggregation between Veillonella species and other oral bacteria. By insertional inactivation of all eight putative hemagglutinin genes, we identified one gene, hag1, which is involved in V. atypica coaggregation with the initial colonizers Streptococcus gordonii, Streptococcus oralis and Streptococcus cristatus, and the periodontal pathogen Porphyromonas gingivalis. The hag1 mutant also abolished adherence to human buccal cells. Inhibition assays using various chemical or physiological treatments suggest different mechanisms being involved in coaggregation with different partners. The entire hag1 gene was sequenced and shown to be the largest known bacterial hemagglutinin gene.
authors.affiliation: Department of Microbiology and Immunology, College of Medicine, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104.
authors.forename: Peng
authors.initials: P
authors.lastname: Zhou
authors.affiliation: Department of Microbiology and Immunology, College of Medicine, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104.
authors.forename: Jinman
authors.initials: J
authors.lastname: Liu
authors.affiliation: Department of Microbiology and Immunology, College of Medicine, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104.|Division of Oral Biology, College of Dentistry, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104.
authors.forename: Justin
authors.initials: J
authors.lastname: Merritt
authors.affiliation: Department of Microbiology and Immunology, College of Medicine, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104.|Division of Oral Biology, College of Dentistry, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104.
authors.forename: Fengxia
authors.initials: F
authors.lastname: Qi
mesh_terms: D001422:Bacterial Adhesion; D001426:Bacterial Proteins / Q000235:genetics / Q000502:physiology; D018441:Biofilms / Q000254:growth & development; D005798:Genes, Bacterial; D006388:Hemagglutinins / Q000235:genetics / Q000502:physiology*; D006801:Humans; D056265:Microbial Interactions; D008969:Molecular Sequence Data; D009055:Mouth / Q000382:microbiology; D009061:Mouth Mucosa / Q000382:microbiology*; D009154:Mutation; D016966:Porphyromonas gingivalis / Q000502:physiology; D017422:Sequence Analysis, DNA; D013291:Streptococcus / Q000502:physiology; D054773:Streptococcus gordonii / Q000502:physiology; D019533:Streptococcus oralis / Q000502:physiology; D000069320:Type V Secretion Systems / Q000235:genetics / Q000502:physiology*; D014678:Veillonella / Q000235:genetics / Q000502:physiology*
chemical_list: D001426:Bacterial Proteins; D006388:Hemagglutinins; D000069320:Type V Secretion Systems
keywords: coaggregation; hag1; hemagglutinin; veillonella