title: On the interaction and localization of the beet necrotic yellow vein virus replicase.
abstract: Beet necrotic yellow vein virus (BNYVV) is a multipartite positive-strand RNA virus. BNYVV RNA-1 encodes a non-structural p237 polyprotein processed in two proteins (p150 and p66) by a cis-acting protease activity. BNYVV non-structural proteins are closely related to replication proteins of positive strand RNA viruses such as hepeviruses rather to other plant virus replicases. The p237 and dsRNA have been localized by TEM in ER structures of infected leaf cells whereas dsRNA was immunolabeled in infected protoplasts. The p150 contains domains with methyltransferase, protease, helicase and two domains of unknown function whereas p66 encompasses the RNA-dependent RNA-polymerase signature. We report the existing interactions between functional domains of the p150 and p66 proteins and the addressing of the benyvirus replicase to the endoplasmic reticulum. Yeast two-hybrid approach, colocalization with FRET-FLIM analyses and co-immunoprecipitation highlighted existing interactions that suggest the presence of a multimeric complex at the vicinity of the cellular membranous web.
authors.affiliation: Institut de biologie moléculaire des plantes du CNRS, conventionné avec l'Université de Strasbourg, 12 rue du général Zimmer, 67084 Strasbourg Cedex, France; Plant Virology Research Center, College of Agriculture, Shiraz University, Shiraz, Iran.
authors.forename: Arezoo
authors.initials: A
authors.lastname: Pakdel
authors.affiliation: Institut de biologie moléculaire des plantes du CNRS, conventionné avec l'Université de Strasbourg, 12 rue du général Zimmer, 67084 Strasbourg Cedex, France.
authors.forename: Claire
authors.initials: C
authors.lastname: Mounier
authors.affiliation: Institut de biologie moléculaire des plantes du CNRS, conventionné avec l'Université de Strasbourg, 12 rue du général Zimmer, 67084 Strasbourg Cedex, France; SESVanderHave, Industriepark 15, Soldatenplein, Z2 3300 Tienen, Belgium.
authors.forename: Elodie
authors.initials: E
authors.lastname: Klein
authors.affiliation: Institut de biologie moléculaire des plantes du CNRS, conventionné avec l'Université de Strasbourg, 12 rue du général Zimmer, 67084 Strasbourg Cedex, France.
authors.forename: Kamal
authors.initials: K
authors.lastname: Hleibieh
authors.affiliation: Institut de biologie moléculaire des plantes du CNRS, conventionné avec l'Université de Strasbourg, 12 rue du général Zimmer, 67084 Strasbourg Cedex, France.
authors.forename: Baptiste
authors.initials: B
authors.lastname: Monsion
authors.affiliation: Institut de biologie moléculaire des plantes du CNRS, conventionné avec l'Université de Strasbourg, 12 rue du général Zimmer, 67084 Strasbourg Cedex, France.
authors.forename: Jérôme
authors.initials: J
authors.lastname: Mutterer
authors.affiliation: Institut de biologie moléculaire des plantes du CNRS, conventionné avec l'Université de Strasbourg, 12 rue du général Zimmer, 67084 Strasbourg Cedex, France.
authors.forename: Mathieu
authors.initials: M
authors.lastname: Erhardt
authors.affiliation: Institut de biologie moléculaire des plantes du CNRS, conventionné avec l'Université de Strasbourg, 12 rue du général Zimmer, 67084 Strasbourg Cedex, France.
authors.forename: Salah
authors.initials: S
authors.lastname: Bouzoubaa
authors.affiliation: DipSA - Plant Pathology, University of Bologna, Viale G. Fanin, 40, 40127 Bologna, Italy.
authors.forename: Claudio
authors.initials: C
authors.lastname: Ratti
authors.affiliation: Institut de biologie moléculaire des plantes du CNRS, conventionné avec l'Université de Strasbourg, 12 rue du général Zimmer, 67084 Strasbourg Cedex, France. Electronic address: gilmer@unistra.fr.
authors.forename: David
authors.initials: D
authors.lastname: Gilmer
mesh_terms: D004721:Endoplasmic Reticulum / Q000378:metabolism; D015870:Gene Expression; D042541:Intracellular Space / Q000378:metabolism; D010935:Plant Diseases / Q000821:virology; D010942:Plant Viruses / Q000502:physiology*; D011485:Protein Binding; D054730:Protein Interaction Domains and Motifs / Q000235:genetics; D021381:Protein Transport; D011523:Protoplasts / Q000378:metabolism; D012330:RNA, Double-Stranded / Q000235:genetics / Q000378:metabolism; D012367:RNA, Viral / Q000235:genetics / Q000378:metabolism; D012324:RNA-Dependent RNA Polymerase / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
chemical_list: D012330:RNA, Double-Stranded; D012367:RNA, Viral; D012324:RNA-Dependent RNA Polymerase
keywords: Benyvirus; Coip; Dsrna; Fret-FLIM; Protein interaction; TEM