title: Low/negative expression of PDGFR-α identifies the candidate primary mesenchymal stromal cells in adult human bone marrow.
abstract: Human bone marrow (BM) contains a rare population of nonhematopoietic mesenchymal stromal cells (MSCs), which are of central importance for the hematopoietic microenvironment. However, the precise phenotypic definition of these cells in adult BM has not yet been reported. In this study, we show that low/negative expression of CD140a (PDGFR-α) on lin(-)/CD45(-)/CD271(+) BM cells identified a cell population with very high MSC activity, measured as fibroblastic colony-forming unit frequency and typical in vitro and in vivo stroma formation and differentiation capacities. Furthermore, these cells exhibited high levels of genes associated with mesenchymal lineages and HSC supportive function. Moreover, lin(-)/CD45(-)/CD271(+)/CD140a(low/-) cells effectively mediated the ex vivo expansion of transplantable CD34(+) hematopoietic stem cells. Taken together, these data indicate that CD140a is a key negative selection marker for adult human BM-MSCs, which enables to prospectively isolate a close to pure population of candidate human adult stroma stem/progenitor cells with potent hematopoiesis-supporting capacity.
authors.affiliation: Lund Stem Cell Center, Lund University, 22184 Lund, Sweden.
authors.forename: Hongzhe
authors.initials: H
authors.lastname: Li
authors.affiliation: Lund Stem Cell Center, Lund University, 22184 Lund, Sweden.
authors.forename: Roshanak
authors.initials: R
authors.lastname: Ghazanfari
authors.affiliation: Lund Stem Cell Center, Lund University, 22184 Lund, Sweden.
authors.forename: Dimitra
authors.initials: D
authors.lastname: Zacharaki
authors.affiliation: Department of Endocrinology, University of Southern Denmark, Molecular Endocrinology Laboratory (KMEB), Odense 5000, Denmark.
authors.forename: Nicholas
authors.initials: N
authors.lastname: Ditzel
authors.affiliation: Centro Nacional de Investigaciones Cardiovasculares Carlos III, Madrid 28029, Spain.
authors.forename: Joan
authors.initials: J
authors.lastname: Isern
authors.affiliation: Lund Stem Cell Center, Lund University, 22184 Lund, Sweden; Department of Hematology, Skåne University Hospital Lund, 22184 Lund, Sweden.
authors.forename: Marja
authors.initials: M
authors.lastname: Ekblom
authors.affiliation: Centro Nacional de Investigaciones Cardiovasculares Carlos III, Madrid 28029, Spain.
authors.forename: Simón
authors.initials: S
authors.lastname: Méndez-Ferrer
authors.affiliation: Department of Endocrinology, University of Southern Denmark, Molecular Endocrinology Laboratory (KMEB), Odense 5000, Denmark; Stem Cell Unit, College of Medicine, King Saud University, Riyadh 11461, Saudi Arabia; Danish Stem Cell Center (DanStem), Panum Institute, University of Copenhagen, Copenhagen 2200, Denmark.
authors.forename: Moustapha
authors.initials: M
authors.lastname: Kassem
authors.affiliation: Lund Stem Cell Center, Lund University, 22184 Lund, Sweden; Department of Hematology, Skåne University Hospital Lund, 22184 Lund, Sweden. Electronic address: stefan.scheding@med.lu.se.
authors.forename: Stefan
authors.initials: S
authors.lastname: Scheding
mesh_terms: D015703:Antigens, CD / Q000378:metabolism; D015415:Biomarkers; D016000:Cluster Analysis; D003114:Colony-Forming Units Assay; D015870:Gene Expression; D020869:Gene Expression Profiling; D006801:Humans; D016130:Immunophenotyping; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism*; D010641:Phenotype; D020796:Receptor, Platelet-Derived Growth Factor alpha / Q000235:genetics* / Q000378:metabolism*; D059467:Transcriptome
chemical_list: D015703:Antigens, CD; D015415:Biomarkers; D020796:Receptor, Platelet-Derived Growth Factor alpha