title: Evaluation of Vitek MS for rapid classification of clinical isolates belonging to Mycobacterium avium complex.
abstract: We evaluated the ability of the Vitek MS system to classify clinical pulmonary Mycobacterium avium complex isolates compared to multilocus sequence analysis. Vitek MS accurately identified 55% of the isolates as M. avium and 18% as M. intracellulare, but misidentified 24 (27%) Mycobacterium chimaera isolates as Mycobacterium intracellulare.
authors.affiliation: Division of Infectious Diseases, Department of Medicine, Northwestern University Feinberg School of Medicine, Chicago, IL, USA. Electronic address: daniel.boyle@northwestern.edu.
authors.forename: Daniel P
authors.initials: DP
authors.lastname: Boyle
authors.affiliation: Division of Infectious Diseases, Department of Medicine, Northwestern University Feinberg School of Medicine, Chicago, IL, USA.
authors.forename: Teresa R
authors.initials: TR
authors.lastname: Zembower
authors.affiliation: Department of Pathology, Northwestern University Feinberg School of Medicine, Chicago, IL, USA.
authors.forename: Chao
authors.initials: C
authors.lastname: Qi
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics; D006801:Humans; D008171:Lung Diseases / Q000382:microbiology; D058885:Multilocus Sequence Typing / Q000379:methods; D015269:Mycobacterium avium Complex / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D015270:Mycobacterium avium-intracellulare Infection / Q000382:microbiology; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000295:instrumentation* / Q000379:methods
chemical_list: D001426:Bacterial Proteins
keywords: Matrix-assisted laser desorption ionization-time of flight mass spectrometry; Multilocus sequencing; Mycobacterium avium complex; Mycobacterium chimaera; Real-time PCR