title: Analysis of intracellular PTEN signaling and secretion.
abstract: The tumor suppressor PTEN dephosphorylates PIP3 to inhibit PI3K signaling in cells. Altering PTEN intracellular signaling can therefore significantly affect cell behavior. Two novel mechanisms of PTEN regulation including the secretion and entry of the translational variant PTEN-L, and enzymatic inhibition by the interacting protein P-REX2, have been shown to modulate PI3K signaling, cellular proliferation and survival, and glucose metabolism. Here, we review the methods used to identify and validate the existence of both PTEN-L and the P-REX2-PTEN complex, to determine their effects on PTEN phosphatase activity, and to examine their role in cellular physiology.
authors.affiliation: Department of Medicine, Weill Cornell Medical College, New York, NY 10065, USA.
authors.forename: Cindy
authors.initials: C
authors.lastname: Hodakoski
authors.affiliation: Department of Medicine, Division of Cardiovascular Medicine, Columbia University Medical Center, New York, NY 10032, USA.
authors.forename: Barry
authors.initials: B
authors.lastname: Fine
authors.affiliation: Department of Medicine, Weill Cornell Medical College, New York, NY 10065, USA.
authors.forename: Benjamin
authors.initials: B
authors.lastname: Hopkins
authors.affiliation: Department of Oncological Sciences, Icahn School of Medicine at Mount Sinai, 1470 Madison Avenue, New York, NY 10029, USA. Electronic address: ramon.parsons@mssm.edu.
authors.forename: Ramon
authors.initials: R
authors.lastname: Parsons
mesh_terms: D000818:Animals; D006801:Humans; D047468:Immunoprecipitation / Q000379:methods; D007424:Intracellular Fluid / Q000378:metabolism*; D051059:PTEN Phosphohydrolase / Q000378:metabolism*; D015398:Signal Transduction / Q000502:physiology*; D025521:Tumor Suppressor Proteins / Q000378:metabolism*
chemical_list: D025521:Tumor Suppressor Proteins; D051059:PTEN Phosphohydrolase; C494929:PTEN protein, human
keywords: P-REX2; PTEN; PTEN-L; Secretion