title: Reprint of "Experimental infection of Opisthorchis viverrini cercariae to the cyprinid fish, Barbonymus gonionotus".
abstract: Opisthorchis viverrini is an important public health problem, a major cause of cholangiocarcinoma in the Greater Mekong subregion including Lao PDR, Cambodia, Vietnam and Thailand. Humans acquire the infection by consumption of raw, fermented or partially cooked freshwater cyprinid fish containing infective metacercariae. This study examined the effect of cercarial infection dosage (25-200 cercariae), age (1-60 day) and size (1-24 mm) of Barbonymus gonionotus fish on infection success of O. viverrini cercariae. Additionally, the site of cyst formation of O. viverrini cercariae and subsequent development to the metacercariae was examined. The result showed that cercarial infection dose (F4,95=8.52, P<0.001) age (P<0.001, ƛLR(2)=954.72) and size (P<0.001, ƛLR(2)=1204.84) were significantly associated with number of O. viverrini metacercariae recovery with in every additional fish age, the O. viverrini metacercarial recovery rate increased by 5.4% (P<0.001). Most metacercariae were found in the body of the fish (81.4%). We concluded that O. viverrini cercarial infection dosage, and age and size of fish were important determinants for a successful infection to fish.
authors.affiliation: Food-borne Parasite Research Group, Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand.
authors.forename: Chalermlap
authors.initials: C
authors.lastname: Donthaisong
authors.affiliation: Food-borne Parasite Research Group, Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand.
authors.forename: Patpicha
authors.initials: P
authors.lastname: Arunsan
authors.affiliation: Food-borne Parasite Research Group, Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand.
authors.forename: Kulwadee
authors.initials: K
authors.lastname: Suwannatrai
authors.affiliation: Food-borne Parasite Research Group, Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand.
authors.forename: Sattrachai
authors.initials: S
authors.lastname: Prasopdee
authors.affiliation: Department of Biology, Faculty of Science, Udon Thani Rajabhat University, Udon Thani 41000, Thailand.
authors.forename: Jutharat
authors.initials: J
authors.lastname: Kulsantiwong
authors.affiliation: Department of Fisheries, Faculty of Agriculture, Khon Kaen University, Khon Kaen 40002, Thailand.
authors.forename: Sutee
authors.initials: S
authors.lastname: Wongmaneeprateep
authors.affiliation: Food-borne Parasite Research Group, Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand.
authors.forename: Apiporn
authors.initials: A
authors.lastname: Suwannatrai
authors.affiliation: Food-borne Parasite Research Group, Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand. Electronic address: smarn_te@kku.ac.th.
authors.forename: Smarn
authors.initials: S
authors.lastname: Tesana
keywords: Barbonymus gonionotus; Infectivity; Metacercariae; Opisthorchis viverrini cercariae