title: Bioenhanced dissolution of dense non-aqueous phase of trichloroethylene as affected by iron reducing conditions: model systems and environmental samples.
abstract: The anaerobic biotransformation of trichloroethylene (TCE) can be affected by competing electron acceptors such as Fe (III). This study assessed the role of Fe (III) reduction on the bioenhanced dissolution of TCE dense non-aqueous phase liquid (DNAPL). Columns were set up as 1-D diffusion cells consisting of a lower DNAPL layer, a layer with an aquifer substratum and an upper water layer that is regularly refreshed. The substrata used were either inert sand or sand coated with 2-line ferrihydrite (HFO) or two environmental Fe (III) containing samples. The columns were inoculated with KB-1 and were repeatedly fed with formate. In none of the diffusion cells, vinyl chloride or ethene was detected while dissolved and extractable Fe (II) increased strongly during 60 d of incubation. The cis-DCE concentration peaked at 4.0 cm from the DNAPL (inert sand) while it was at 3.4 cm (sand+HFO), 1.7 cm and 2.5 cm (environmental samples). The TCE concentration gradients near the DNAPL indicate that the DNAPL dissolution rate was larger than that in an abiotic cell by factors 1.3 (inert sand), 1.0 (sand+HFO) and 2.2 (both environmental samples). This results show that high bioavailable Fe (III) in HFO reduces the TCE degradation by competitive Fe (III) reduction, yielding lower bioenhanced dissolution. However, Fe (III) reduction in environmental samples was not reducing TCE degradation and the dissolution factor was even larger than that of inert sand. It is speculated that physical factors, e.g. micro-niches in the environmental samples protect microorganisms from toxic concentrations of TCE.
authors.affiliation: Division of Soil and Water Management, Department of Earth and Environmental Sciences, KU Leuven, Kasteelpark Arenberg 20, B-3001 Heverlee, Belgium.
authors.forename: Laiby
authors.initials: L
authors.lastname: Paul
authors.affiliation: Division of Soil and Water Management, Department of Earth and Environmental Sciences, KU Leuven, Kasteelpark Arenberg 20, B-3001 Heverlee, Belgium. Electronic address: Erik.Smolders@ees.kuleuven.be.
authors.forename: Erik
authors.initials: E
authors.lastname: Smolders
mesh_terms: D001673:Biodegradation, Environmental; D041862:Chloroflexi / Q000378:metabolism; D004058:Diffusion; D005290:Ferric Compounds / Q000737:chemistry; D045302:Geobacter / Q000378:metabolism; D060587:Groundwater; D007501:Iron / Q000737:chemistry*; D010084:Oxidation-Reduction; D012822:Silicon Dioxide / Q000737:chemistry; D012995:Solubility; D014241:Trichloroethylene / Q000737:chemistry / Q000378:metabolism*; D014874:Water Pollutants, Chemical / Q000737:chemistry* / Q000378:metabolism*
chemical_list: D005290:Ferric Compounds; D014874:Water Pollutants, Chemical; D014241:Trichloroethylene; D012822:Silicon Dioxide; C092844:ferric oxyhydroxide; D007501:Iron
keywords: Bioenhanced dissolution; DNAPL; Diffusion; Fe (III) oxides; Reductive dechlorination; Trichloroethylene