title: One-step synthesis of redox-active polymer/AU nanocomposites for electrochemical immunoassay of multiplexed tumor markers.
abstract: In this work, a simple and sensitive multiplexed immunoassay protocol for simultaneous electrochemical determination of alpha-fetoprotein (AFP) and carcinoembryonic antigen (CEA) was designed using redox-active nanocomposites. As the redox-active species, the poly(o-phenylenediamine) (POPD)/Au nanocomposite and poly(vinyl ferrocene-2-aminothiophenol) (poly(VFc-ATP))/Au nanocomposite were obtained by one-step method which HAuCl4 was used as the oxidant. With Au nanoparticles (AuNPs), the nanocomposites were successful to immobilize labeled anti-CEA and anti-AFP as the immunosensing probes. The proposed electrochemical immunoassay enabled the simultaneous monitoring of AFP and CEA in a wide range of 0.01-100ngmL(-1). The detection limits was 0.006ngmL(-1) for CEA and 0.003ngmL(-1) for AFP (S/N=3). The assay results of serum samples with the proposed method were well consistent with the reference values from standard ELISA method. And the negligible cross-reactivity between the two analytes makes it possesses potential promise in clinical diagnosis.
authors.affiliation: Department of Chemistry, Capital Normal University, Beijing 100048, China.
authors.forename: Zhimin
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Department of Chemistry, Capital Normal University, Beijing 100048, China.
authors.forename: Qinfeng
authors.initials: Q
authors.lastname: Rong
authors.affiliation: Department of Chemistry, Capital Normal University, Beijing 100048, China. Electronic address: mazhanfang@cnu.edu.cn.
authors.forename: Zhanfang
authors.initials: Z
authors.lastname: Ma
authors.affiliation: Department of Chemistry, Capital Normal University, Beijing 100048, China. Electronic address: hanhongliang@cnu.edu.cn.
authors.forename: Hongliang
authors.initials: H
authors.lastname: Han
mesh_terms: D000814:Aniline Compounds / Q000138:chemical synthesis / Q000737:chemistry; D015374:Biosensing Techniques / Q000379:methods*; D002272:Carcinoembryonic Antigen / Q000097:blood*; D055664:Electrochemical Techniques / Q000379:methods; D005296:Ferrous Compounds / Q000138:chemical synthesis / Q000737:chemistry*; D006046:Gold / Q000737:chemistry*; D006801:Humans; D007118:Immunoassay / Q000379:methods; D057230:Limit of Detection; D053768:Metal Nanoparticles / Q000737:chemistry / Q000648:ultrastructure; D053761:Nanocomposites / Q000737:chemistry* / Q000648:ultrastructure; D010084:Oxidation-Reduction; D010655:Phenylenediamines / Q000138:chemical synthesis / Q000737:chemistry*; D011145:Polyvinyls / Q000138:chemical synthesis / Q000737:chemistry*; D000509:alpha-Fetoproteins / Q000032:analysis*
chemical_list: D000814:Aniline Compounds; D002272:Carcinoembryonic Antigen; D005296:Ferrous Compounds; D010655:Phenylenediamines; D011145:Polyvinyls; D000509:alpha-Fetoproteins; C530487:poly(o-phenylenediamine); C451099:polyvinylferrocenium; C005464:2-aminothiophenol; D006046:Gold
keywords: Alpha-fetoprotein; Carcinoembryonic antigen; Electrochemical immunosensor; Poly(o-phenylenediamine)/Au nanocomposite; Poly(vinyl ferrocene-2-aminothiophenol)/Au nanocomposite