title: Highly selective "Off-On" fluorescent probe for histidine and its imaging in living cells.
abstract: A naphthalimide-based fluorescent probe CP has been synthesized with simple steps. It can selectively and sensitively recognize copper ions (Cu(2+)) in HEPES buffer (50mM, pH 7.2). The fluorescence intensity of CP is linearly proportional to the concentration of Cu(2+) ranging from 0-8.3μM (correlation coefficient R(2)=0.9808). The resulted complex CP@Cu can serve as a turn-on fluorescent probe for the detection of histidine and histidine rich proteins in broad pH application range. Upon the addition of histidine, the fluorescence intensity of CP@Cu exhibits a linear correlation with the concentration of histidine ranging from 0-200μM (correlation coefficient R(2)=0.9912). Moreover, CP@Cu has potential for imaging histidine in vitro experiments and has promise in real sample applications with great validity.
authors.affiliation: State Key Laboratory of Bioreactor Engineering, Shanghai Key Laboratory of Chemical Biology, School of Pharmacy, East China University of Science and Technology, Shanghai 200237, China.
authors.forename: Tiantian
authors.initials: T
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Bioreactor Engineering, Shanghai Key Laboratory of Chemical Biology, School of Pharmacy, East China University of Science and Technology, Shanghai 200237, China.
authors.forename: Liyan
authors.initials: L
authors.lastname: Yin
authors.affiliation: State Key Laboratory of Bioreactor Engineering, Shanghai Key Laboratory of Chemical Biology, School of Pharmacy, East China University of Science and Technology, Shanghai 200237, China; The Education Ministry Key Laboratory of Resource Chemistry and Shanghai Key Laboratory of Rare Earth Functional Materials, Department of Chemistry, College of Life and Environmental Sciences, Shanghai Normal University, 100 Guilin Road, Shanghai 200234, China. Electronic address: huangcs@shnu.edu.cn.
authors.forename: Chusen
authors.initials: C
authors.lastname: Huang
authors.affiliation: State Key Laboratory of Bioreactor Engineering, Shanghai Key Laboratory of Chemical Biology, School of Pharmacy, East China University of Science and Technology, Shanghai 200237, China.
authors.forename: Yiqiao
authors.initials: Y
authors.lastname: Qin
authors.affiliation: State Key Laboratory of Bioreactor Engineering, Shanghai Key Laboratory of Chemical Biology, School of Pharmacy, East China University of Science and Technology, Shanghai 200237, China. Electronic address: wpzhu@ecust.edu.cn.
authors.forename: Weiping
authors.initials: W
authors.lastname: Zhu
authors.affiliation: State Key Laboratory of Bioreactor Engineering, Shanghai Key Laboratory of Chemical Biology, School of Pharmacy, East China University of Science and Technology, Shanghai 200237, China.
authors.forename: Yufang
authors.initials: Y
authors.lastname: Xu
authors.affiliation: State Key Laboratory of Bioreactor Engineering, Shanghai Key Laboratory of Chemical Biology, School of Pharmacy, East China University of Science and Technology, Shanghai 200237, China.
authors.forename: Xuhong
authors.initials: X
authors.lastname: Qian
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D058948:Cell Tracking; D003300:Copper / Q000737:chemistry; D005456:Fluorescent Dyes; D006639:Histidine / Q000737:chemistry / Q000302:isolation & purification*; D006801:Humans; D057054:Molecular Imaging / Q000379:methods*; D011506:Proteins / Q000302:isolation & purification*
chemical_list: D005456:Fluorescent Dyes; D011506:Proteins; D006639:Histidine; D003300:Copper
keywords: Copper ion; Fluorescent probe; Histidine; Live cell imaging; Naphthalimide