title: Rapid construction of a Bacterial Artificial Chromosomal (BAC) expression vector using designer DNA fragments.
abstract: Bacterial artificial chromosomal (BAC) vectors are increasingly being used in cloning large DNA fragments containing complex biosynthetic pathways to facilitate heterologous production of microbial metabolites for drug development. To express inserted genes using Streptomyces species as the production hosts, an integration expression cassette is required to be inserted into the BAC vector, which includes genetic elements encoding a phage-specific attachment site, an integrase, an origin of transfer, a selection marker and a promoter. Due to the large sizes of DNA inserted into the BAC vectors, it is normally inefficient and time-consuming to assemble these fragments by routine PCR amplifications and restriction-ligations. Here we present a rapid method to insert fragments to construct BAC-based expression vectors. A DNA fragment of about 130 bp was designed, which contains upstream and downstream homologous sequences of both BAC vector and pIB139 plasmid carrying the whole integration expression cassette. In-Fusion cloning was performed using the designer DNA fragment to modify pIB139, followed by λ-RED-mediated recombination to obtain the BAC-based expression vector. We demonstrated the effectiveness of this method by rapid construction of a BAC-based expression vector with an insert of about 120 kb that contains the entire gene cluster for biosynthesis of immunosuppressant FK506. The empty BAC-based expression vector constructed in this study can be conveniently used for construction of BAC libraries using either microbial pure culture or environmental DNA, and the selected BAC clones can be directly used for heterologous expression. Alternatively, if a BAC library has already been constructed using a commercial BAC vector, the selected BAC vectors can be manipulated using the method described here to get the BAC-based expression vectors with desired gene clusters for heterologous expression. The rapid construction of a BAC-based expression vector facilitates heterologous expression of large gene clusters for drug discovery.
authors.affiliation: School of Life Science and Biotechnology, Dalian University of Technology, Dalian 116024, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Chen
authors.affiliation: School of Life Science and Biotechnology, Dalian University of Technology, Dalian 116024, China. Electronic address: xqzhao@dlut.edu.cn.
authors.forename: Xinqing
authors.initials: X
authors.lastname: Zhao
authors.affiliation: Division of Bioscience and Bioinformatics, Myongji University, Yongin 449-728, Korea.
authors.forename: Yingyu
authors.initials: Y
authors.lastname: Jin
authors.affiliation: Department of Biotechnology, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.
authors.forename: Zongbao Kent
authors.initials: ZK
authors.lastname: Zhao
authors.affiliation: Division of Bioscience and Bioinformatics, Myongji University, Yongin 449-728, Korea. Electronic address: jwsuh@mju.ac.kr.
authors.forename: Joo-Won
authors.initials: JW
authors.lastname: Suh
mesh_terms: D022202:Chromosomes, Artificial, Bacterial / Q000235:genetics*; D003001:Cloning, Molecular / Q000379:methods*; D005822:Genetic Vectors; D059765:Homologous Recombination; D005810:Multigene Family; D016559:Tacrolimus / Q000378:metabolism
chemical_list: D016559:Tacrolimus
keywords: Bacterial Artificial Chromosomal (BAC) vector; Biosynthetic gene cluster; Heterologous expression; In-Fusion cloning; Natural product; λ-RED-mediated homologous recombination