title: Real-time chemiluminescence imaging using nano-lantern probes.
abstract: Chemiluminescence imaging can be performed without excitation light sources at various spatial levels ranging from a single cell to the whole body. Thus far, chemiluminescence imaging has been primarily performed with long exposure times because of weak signals, resulting in low temporal resolution. Recently, the brightest-known chemiluminescent proteins--Nano-lantern and Nano-lantern-based functional indicators--have been developed. Nano-lantern probes break the limitation of temporal resolution and enable chemiluminescence imaging of living samples such as cells, plants, and small animals at video rates. This unit describes one protocol for observation of a freely moving unshaved mouse transplanted with Nano-lantern-expressing tumor cells, and another for compatible use of optogenetic tools and a Nano-lantern calcium indicator. Both protocols utilize the synchronization of illumination and camera acquisition sessions, thereby enabling real-time chemiluminescence imaging.
authors.affiliation: The Institute of Scientific and Industrial Research, Osaka University, Osaka, Japan.
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Arai
authors.affiliation: The Institute of Scientific and Industrial Research, Osaka University, Osaka, Japan.
authors.forename: Takeharu
authors.initials: T
authors.lastname: Nagai
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism*; D045744:Cell Line, Tumor; D008156:Luciferases / Q000378:metabolism*; D008163:Luminescent Measurements / Q000379:methods; D008164:Luminescent Proteins / Q000378:metabolism*; D051379:Mice; D057054:Molecular Imaging / Q000379:methods; D062308:Optogenetics / Q000379:methods; D011993:Recombinant Fusion Proteins / Q000378:metabolism*
chemical_list: D008164:Luminescent Proteins; D011993:Recombinant Fusion Proteins; D008156:Luciferases; C581563:nano-lantern protein; D002118:Calcium
keywords: CCD camera; Nano-lantern; Nano-lantern (Ca2+); channelrhodopsin2 (ChR2); dead time; optogenetics