title: A closed loop for municipal organic solid waste by lactic acid fermentation.
abstract: In order to investigate the feasibility of producing lactic acid from municipal organic solid waste different pH values (4-7) and temperatures (37°C and 55°C) were tested. For the evaluation of fermentation conditions the chemical, physical, and microbial characters were monitored over a period of 7days. Quantitative real time PCR, PCR-DGGE, and next generation sequencing of a 16S rRNA gene library were applied to identify the key players of the lactic acid production and their association. Lactobacillus acidophilus and its closest relatives were found to be efficient lactic acid producers (>300mM) under most suitable fermentation conditions tested in this study: 37°C with either uncontrolled pH or at a pH of 5. These data provide the first step in the realization of the idea "reuse, reduce, and recycle" of municipal organic solid waste.
authors.affiliation: Institute of Microbiology, University of Innsbruck, Technikerstraße 25d, 6020 Innsbruck, Austria. Electronic address: Maraike.Probst@uibk.ac.at.
authors.forename: Maraike
authors.initials: M
authors.lastname: Probst
authors.affiliation: Department of Statistics, University of Innsbruck, Universitätsstraße 15, 6020 Innsbruck, Austria. Electronic address: Janette.Walde@uibk.ac.at.
authors.forename: Janette
authors.initials: J
authors.lastname: Walde
authors.affiliation: Institute of Microbiology, University of Innsbruck, Technikerstraße 25d, 6020 Innsbruck, Austria. Electronic address: Thomas.Puempel@uibk.ac.at.
authors.forename: Thomas
authors.initials: T
authors.lastname: Pümpel
authors.affiliation: Institute of Microbiology, University of Innsbruck, Technikerstraße 25d, 6020 Innsbruck, Austria. Electronic address: Andreas.Wagner@uibk.ac.at.
authors.forename: Andreas Otto
authors.initials: AO
authors.lastname: Wagner
authors.affiliation: Institute of Microbiology, University of Innsbruck, Technikerstraße 25d, 6020 Innsbruck, Austria. Electronic address: Heribert.Insam@uibk.ac.at.
authors.forename: Heribert
authors.initials: H
authors.lastname: Insam
mesh_terms: D005285:Fermentation; D007220:Industrial Waste; D019344:Lactic Acid / Q000378:metabolism*; D012037:Refuse Disposal / Q000379:methods*; D062611:Solid Waste
chemical_list: D007220:Industrial Waste; D062611:Solid Waste; D019344:Lactic Acid
keywords: Fermentation; Lactic acid bacteria; Lactobacillus; Organic residues; Unsterilized biowaste