title: Effect of protons on the mechanical response of rat muscle nociceptive fibers and neurons in vitro.
abstract: Strong exercise makes muscle acidic, and painful. The stimulus that activates muscle nociceptors in such instance may be protons. Reportedly, however, not many afferents are excited by protons alone. We, therefore, posited that protons sensitize muscular nociceptors to mechanical stimuli. We examined effects of protons on mechanical sensitivity of muscle nociceptors by single-fiber recording from rat muscle-nerve preparations in vitro and by whole cell patch-clamp recording of mechanically activated (MA) currents from cultured rat dorsal root ganglion neurons. We recorded 38 Aδ- and C-fibers. Their response magnitude was increased by both pH 6.2 and pH 6.8; in addition the mechanical threshold was lowered by pH 6.2. Decrease in the threshold by pH6.2 was also observed in MA currents. Presently observed sensitization by protons could be involved in several types of ischemic muscle pain, and may also be involved in cardiovascular and respiratory controls during exercise.
authors.affiliation: Department of Neuroscience II, Research Institute of Environmental Medicine, Nagoya University, Nagoya 464-8601, Japan.
authors.forename: Norio
authors.initials: N
authors.lastname: Hotta
authors.affiliation: Department of Neuroscience II, Research Institute of Environmental Medicine, Nagoya University, Nagoya 464-8601, Japan.
authors.forename: Asako
authors.initials: A
authors.lastname: Kubo
authors.affiliation: Department of Neuroscience II, Research Institute of Environmental Medicine, Nagoya University, Nagoya 464-8601, Japan. Electronic address: mizu@isc.chubu.ac.jp.
authors.forename: Kazue
authors.initials: K
authors.lastname: Mizumura
mesh_terms: D000818:Animals; D005727:Ganglia, Spinal / Q000502:physiology*; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D008297:Male; D018482:Muscle, Skeletal / Q000294:innervation; D009474:Neurons / Q000502:physiology*; D009619:Nociceptors / Q000502:physiology*; D010543:Peroneal Nerve / Q000502:physiology*; D010812:Physical Stimulation; D011522:Protons; D051381:Rats; D017207:Rats, Sprague-Dawley; D013268:Stimulation, Chemical
chemical_list: D011522:Protons
keywords: Acid; Exercise; Ischemic pain; Mechanical sensitization; Muscle pain