title: Bone regeneration in experimental animals using calcium phosphate cement combined with platelet growth factors and human growth hormone.
abstract: Many substances (growth factors and hormones) have osteoinduction properties and when added to some osteoconduction biomaterial they accelerate bone neoformation properties.
  
  
  METHODS
  The materials included 15 New Zealand rabbits, calcium phosphate cement (Calcibon(®)), human growth hormone (GH), and plasma rich in platelets (PRP).
  
  
  METHODS
  Each animal was operated on in both proximal tibias and a critical size bone defect of 6mm of diameter was made. The animals were separated into the following study groups: Control (regeneration only by Calcibon®), PRP (regeneration by Calcibon® and PRP), GH (regeneration by Calcibon® and GH). All the animals were sacrificed at 28 days. An evaluation was made of the appearance of the proximal extreme of rabbit tibiae in all the animals, and to check the filling of the critical size defect. A histological assessment was made of the tissue response, the presence of new bone formation, and the appearance of the biomaterial. Morphometry was performed using the MIP 45 image analyser. ANOVA statistical analysis was performed using the Statgraphics software application.
  
  
  RESULTS
  The macroscopic appearance of the critical defect was better in the PRP and the GH group than in the control group. Histologically greater new bone formation was found in the PRP and GH groups. No statistically significant differences were detected in the morphometric study between bone formation observed in the PRP group and the control group. Significant differences in increased bone formation were found in the GH group (p=0.03) compared to the other two groups.
  
  
  CONCLUSIONS
  GH facilitates bone regeneration in critical defects filled with calcium phosphate cement in the time period studied in New Zealand rabbits.
authors.affiliation: Servicio de Traumatología, Hospital Universitario Puerta de Hierro, Majadahonda, Madrid, España. Electronic address: kaloyanvelev@yahoo.es.
authors.forename: K
authors.initials: K
authors.lastname: Emilov-Velev
authors.affiliation: Unidad de Embriología y Anatomía Humana, Facultad de Medicina, Universidad de Alcalá, Alcalá de Henares, Madrid, España.
authors.forename: C
authors.initials: C
authors.lastname: Clemente-de-Arriba
authors.affiliation: Práctica privda, Alcalá de Henares, Madrid, España.
authors.forename: M Á
authors.initials: MÁ
authors.lastname: Alobera-García
authors.affiliation: Centro de Atención Primaria María de Guzmán, Alcalá de Henares, Madrid, España.
authors.forename: E M
authors.initials: EM
authors.lastname: Moreno-Sansalvador
authors.affiliation: Servicio de Traumatología, Hospital Universitario Puerta de Hierro, Majadahonda, Madrid, España.
authors.forename: J
authors.initials: J
authors.lastname: Campo-Loarte
mesh_terms: D000818:Animals; D001672:Biocompatible Materials; D001843:Bone Cements / Q000627:therapeutic use*; D001861:Bone Regeneration; D002130:Calcium Phosphates / Q000627:therapeutic use*; D048091:Guided Tissue Regeneration / Q000379:methods*; D019382:Human Growth Hormone / Q000627:therapeutic use*; D006801:Humans; D008297:Male; D053657:Platelet-Rich Plasma; D011817:Rabbits; D013977:Tibia / Q000293:injuries* / Q000473:pathology / Q000601:surgery; D016896:Treatment Outcome
chemical_list: D001672:Biocompatible Materials; D001843:Bone Cements; D002130:Calcium Phosphates; D019382:Human Growth Hormone; C020243:calcium phosphate
keywords: Bone regeneration; Calcium phosphate cements; Cemento de fosfato cálcico; Critical bone defect; Defecto óseo crítico; Growth hormone; Hormona de crecimiento; Morfometría; Morphometry; Plasma rico en plaquetas; Platelet-rich plasma; Regeneración ósea