title: Angiotensin-(1-7) in the basolateral amygdala attenuates the cardiovascular response evoked by acute emotional stress.
abstract: The basolateral amygdala (BLA) plays a critical role in mediating physiological responses to emotional stress. Recent data suggest that angiotensin-(1-7) [Ang-(1-7)] can act centrally attenuating the cardiovascular response to acute stress. We investigated whether Ang-(1-7) in the BLA plays a role in the cardiovascular response to emotional stress. Under anesthesia, guide cannulas were implanted into the BLA of Wistar rats. Five days later, the femoral artery was cannulated for mean arterial pressure (MAP) and heart rate (HR) recordings. Microinjections of Ang-(1-7) (5 or 50 pmol), the Mas receptor antagonist A-779 (100 pmol), Ang-(1-7)+A-779 (50 + 100 pmol, respectively), or vehicle (NaCl 0.9%, control) were performed after 24h and rats were then submitted to stress trials. Injection of Ang-(1-7) into the BLA blocked the tachycardia (ΔHR: vehicle 135 ± 23 vs. Ang-(1-7) 9 ± 12 bpm; P<0.05) and the pressor response (ΔMAP: vehicle 28 ± 3 mmHg vs. Ang-(1-7) 6 ± 2 mmHg; P<0.05) produced by air jet stress. These effects were completely reversed by A-779 (ΔHR: 109 ± 11 bpm; ΔMAP: 18 ± 2 mmHg). Ang-(1-7) into the BLA also attenuated the pressor response evoked by cage-switch stress paradigm. These findings indicate that Ang-(1-7) can act in the BLA through the Mas receptors modulating the cardiovascular response evoked by emotional stress.
authors.affiliation: National Institute of Science and Technology in Nanobiopharmaceutics (INCT - Nanobiofar), Laboratório de Hipertensão, Departamento de Fisiologia e Biofísica, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.
authors.forename: Charles Gonzaga
authors.initials: CG
authors.lastname: Oscar
authors.affiliation: National Institute of Science and Technology in Nanobiopharmaceutics (INCT - Nanobiofar), Laboratório de Hipertensão, Departamento de Fisiologia e Biofísica, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.
authors.forename: Flávia Camargos de Figueirêdo
authors.initials: FC
authors.lastname: Müller-Ribeiro
authors.affiliation: National Institute of Science and Technology in Nanobiopharmaceutics (INCT - Nanobiofar), Laboratório de Hipertensão, Departamento de Fisiologia e Biofísica, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.
authors.forename: Lidiane Gonzaga
authors.initials: LG
authors.lastname: de Castro
authors.affiliation: National Institute of Science and Technology in Nanobiopharmaceutics (INCT - Nanobiofar), Laboratório de Hipertensão, Departamento de Fisiologia e Biofísica, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.
authors.forename: Augusto
authors.initials: A
authors.lastname: Martins Lima
authors.affiliation: National Institute of Science and Technology in Nanobiopharmaceutics (INCT - Nanobiofar), Laboratório de Hipertensão, Departamento de Fisiologia e Biofísica, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.
authors.forename: Maria José
authors.initials: MJ
authors.lastname: Campagnole-Santos
authors.affiliation: National Institute of Science and Technology in Nanobiopharmaceutics (INCT - Nanobiofar), Laboratório de Hipertensão, Departamento de Fisiologia e Biofísica, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.
authors.forename: Robson Augusto Souza
authors.initials: RA
authors.lastname: Santos
authors.affiliation: Laboratório de Fisiologia e Terapêutica Cardiovascular, Departamento de Ciências Fisiológicas. Instituto de Ciências Biológicas, Universidade Federal de Goiás, Goiânia, GO, Brazil.
authors.forename: Carlos Henrique
authors.initials: CH
authors.lastname: Xavier
authors.affiliation: National Institute of Science and Technology in Nanobiopharmaceutics (INCT - Nanobiofar), Laboratório de Hipertensão, Departamento de Fisiologia e Biofísica, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil. Electronic address: peliky@icb.ufmg.br.
authors.forename: Marco Antônio Peliky
authors.initials: MA
authors.lastname: Fontes
mesh_terms: D000679:Amygdala / Q000187:drug effects / Q000378:metabolism*; D000803:Angiotensin I / Q000378:metabolism* / Q000494:pharmacology; D000818:Animals; D002319:Cardiovascular System / Q000187:drug effects*; D006439:Hemodynamics / Q000187:drug effects / Q000502:physiology*; D008297:Male; D010446:Peptide Fragments / Q000378:metabolism* / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D013315:Stress, Psychological / Q000378:metabolism* / Q000503:physiopathology
chemical_list: D010446:Peptide Fragments; D000803:Angiotensin I; C118790:angiotensin I (1-7)
keywords: Amygdala; Angiotensin; Cardiovascular; Emotional stress