title: Koolpinyah and Yata viruses: two newly recognised ephemeroviruses from tropical regions of Australia and Africa.
abstract: Koolpinyah virus (KOOLV) isolated from healthy Australian cattle and Yata virus (YATV) isolated from a pool of Mansonia uniformis mosquitoes in the Central African Republic have been tentatively identified as rhabdoviruses. KOOLV was shown previously to be related antigenically to kotonkon virus, an ephemerovirus that has caused an ephemeral fever-like illness in cattle in Nigeria, but YATV failed to react antigenically with any other virus tested. Here we report the complete genome sequences of KOOLV (16,133 nt) and YATV (14,479 nt). Each has a complex genome organisation, with multiple genes, including a second non-structural glycoprotein (GNS) gene and a viroporin (α1) gene, between the G and L genes as is characteristic of ephemeroviruses. Based on an analysis of genome organisation, sequence identity and cross-neutralisation, we demonstrate that both KOOLV and YATV should be classified as two new species in the genus Ephemerovirus.
authors.affiliation: CSIRO Animal, Food and Health Sciences, Australian Animal Health Laboratory, 5 Portarlington Road, Geelong, VIC 3220, Australia.
authors.forename: Kim R
authors.initials: KR
authors.lastname: Blasdell
authors.affiliation: Department of Biochemistry and Molecular Biology, The University of Texas Medical Branch, Galveston, TX, USA.
authors.forename: Steven G
authors.initials: SG
authors.lastname: Widen
authors.affiliation: CSIRO Animal, Food and Health Sciences, Australian Animal Health Laboratory, 5 Portarlington Road, Geelong, VIC 3220, Australia.
authors.forename: Sinéad M
authors.initials: SM
authors.lastname: Diviney
authors.affiliation: CSIRO Animal, Food and Health Sciences, Australian Animal Health Laboratory, 5 Portarlington Road, Geelong, VIC 3220, Australia.
authors.forename: Cadhla
authors.initials: C
authors.lastname: Firth
authors.affiliation: Department of Biochemistry and Molecular Biology, The University of Texas Medical Branch, Galveston, TX, USA.
authors.forename: Thomas G
authors.initials: TG
authors.lastname: Wood
authors.affiliation: Center for Biodefense and Emerging Infectious Diseases and Department of Pathology, Center for Tropical Diseases, and Institute for Human Infections and Immunity, The University of Texas Medical Branch, Galveston, TX, USA.
authors.forename: Hilda
authors.initials: H
authors.lastname: Guzman
authors.affiliation: Marie Bashir Institute for Infectious Diseases & Biosecurity, Charles Perkins Centre, School of Biological Sciences and Sydney Medical School, The University of Sydney, Sydney, NSW 2006, Australia.
authors.forename: Edward C
authors.initials: EC
authors.lastname: Holmes
authors.affiliation: Center for Biodefense and Emerging Infectious Diseases and Department of Pathology, Center for Tropical Diseases, and Institute for Human Infections and Immunity, The University of Texas Medical Branch, Galveston, TX, USA.
authors.forename: Robert B
authors.initials: RB
authors.lastname: Tesh
authors.affiliation: Center for Biodefense and Emerging Infectious Diseases and Department of Pathology, Center for Tropical Diseases, and Institute for Human Infections and Immunity, The University of Texas Medical Branch, Galveston, TX, USA.
authors.forename: Nikos
authors.initials: N
authors.lastname: Vasilakis
authors.affiliation: CSIRO Animal, Food and Health Sciences, Australian Animal Health Laboratory, 5 Portarlington Road, Geelong, VIC 3220, Australia. Electronic address: Peter.Walker@csiro.au.
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Walker
mesh_terms: D000349:Africa / Q000453:epidemiology; D000595:Amino Acid Sequence; D000818:Animals; D001315:Australia / Q000453:epidemiology; D001483:Base Sequence; D002417:Cattle; D002418:Cattle Diseases / Q000453:epidemiology / Q000821:virology*; D009033:Culicidae / Q000821:virology; D004810:Ephemeral Fever / Q000453:epidemiology / Q000821:virology*; D019235:Ephemerovirus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D016679:Genome, Viral / Q000235:genetics*; D008969:Molecular Sequence Data; D010802:Phylogeny; D012209:Rhabdoviridae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D018353:Rhabdoviridae Infections / Q000453:epidemiology / Q000821:virology*; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA / Q000662:veterinary
keywords: Ephemerovirus; Genome sequence; Koolpinyah virus; Phylogeny; Rhabdovirus; Yata virus