title: Feasibility study of an active wound dressing based on hollow fiber membranes in a porcine wound model.
abstract: METHODS
  A novel active wound dressing (AWD) concept based on a microporous hollow fiber membrane network was investigated in an animal model. It provides a local solution-perfused environment for regenerative cell nutrition, wound irrigation, debris removal, electrolyte balancing, pH regulation, and topical antibiosis. The device is capable of supplying soluble factors, as tested experimentally for the recombinant human growth and differentiation factor-5 (rhGDF-5).
  
  
  METHODS
  Following in vitro studies for rhGDF-5 using primary human keratinocytes and dermal fibroblasts, we employed a porcine partial thickness wound model with five distinct wounds on each back of n=8 pigs. Four wound groups were perfused differently over 9 days and compared with a negative control wound without perfusion: (1) 1% trehalose solution, pH 5.5; (2) rhGDF-5 (150 ng/ml) in 1% trehalose solution, pH 5.5; (3) nutrition solution; and (4) rhGDF-5 (150 ng/ml) in nutrition solution with 1% trehalose, pH 5.5.
  
  
  RESULTS
  Promoted wound healing was observed within group 1 and more pronounced within group 2. Groups 3 and 4, with nutrition solution, showed significant adverse effects on wound healing (p<0.05).
  
  
  CONCLUSIONS
  The investigated AWD concept appears to be an interesting therapeutic tool to study further wound healing support. Additionally, topical application of rhGDF-5 could be promising.
authors.affiliation: AG Experimental Surgery, BCRT, Charité Campus Virchow-Klinikum, Universitätsmedizin Berlin, Germany.
authors.forename: Jörn
authors.initials: J
authors.lastname: Plettig
authors.affiliation: AG Experimental Surgery, BCRT, Charité Campus Virchow-Klinikum, Universitätsmedizin Berlin, Germany.
authors.forename: Christa M
authors.initials: CM
authors.lastname: Johnen
authors.affiliation: AG Experimental Surgery, BCRT, Charité Campus Virchow-Klinikum, Universitätsmedizin Berlin, Germany.
authors.forename: Kirsten
authors.initials: K
authors.lastname: Bräutigam
authors.affiliation: AG Experimental Surgery, BCRT, Charité Campus Virchow-Klinikum, Universitätsmedizin Berlin, Germany.
authors.forename: Fanny
authors.initials: F
authors.lastname: Knöspel
authors.affiliation: AG Experimental Surgery, BCRT, Charité Campus Virchow-Klinikum, Universitätsmedizin Berlin, Germany.
authors.forename: Eva C
authors.initials: EC
authors.lastname: Wönne
authors.affiliation: StemCell Systems GmbH, Berlin, Germany.
authors.forename: Frank
authors.initials: F
authors.lastname: Schubert
authors.affiliation: Biopharm GmbH, Heidelberg, Germany.
authors.forename: Frank
authors.initials: F
authors.lastname: Plöger
authors.affiliation: Department of Experimental Medicine (FEM), Charité Campus Virchow-Klinikum, Universitätsmedizin Berlin, Germany.
authors.forename: Juliane K
authors.initials: JK
authors.lastname: Unger
authors.affiliation: Department of General, Visceral, and Transplantation Surgery, Charité Campus Virchow-Klinikum, Universitätsmedizin Berlin, Germany.
authors.forename: Anja
authors.initials: A
authors.lastname: Reutzel-Selke
authors.affiliation: StemCell Systems GmbH, Berlin, Germany.
authors.forename: Reinhard
authors.initials: R
authors.lastname: Bornemann
authors.affiliation: AG Experimental Surgery, BCRT, Charité Campus Virchow-Klinikum, Universitätsmedizin Berlin, Germany.
authors.forename: Katrin
authors.initials: K
authors.lastname: Zeilinger
authors.affiliation: McGowan Institute for Regenerative Medicine, Departments of Surgery and Bioengineering, University of Pittsburgh, Pittsburgh, PA, USA. Electronic address: joerg.gerlach@cellnet.org.
authors.forename: Jörg C
authors.initials: JC
authors.lastname: Gerlach
mesh_terms: D000287:Administration, Topical; D000818:Animals; D000898:Antibiosis / Q000187:drug effects; D001458:Bandages; D002056:Burns / Q000628:therapy*; D002478:Cells, Cultured; D005240:Feasibility Studies; D005347:Fibroblasts / Q000187:drug effects*; D055428:Growth Differentiation Factor 5 / Q000494:pharmacology*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D015603:Keratinocytes / Q000187:drug effects*; D019999:Pharmaceutical Solutions / Q000494:pharmacology; D062326:Re-Epithelialization / Q000187:drug effects; D011994:Recombinant Proteins; D019346:Sodium Acetate / Q000494:pharmacology*; D012996:Solutions; D013552:Swine; D007507:Therapeutic Irrigation; D014199:Trehalose / Q000494:pharmacology*; D014945:Wound Healing / Q000187:drug effects*
chemical_list: C525893:GDF5 protein, human; D055428:Growth Differentiation Factor 5; D019999:Pharmaceutical Solutions; D011994:Recombinant Proteins; D012996:Solutions; D019346:Sodium Acetate; D014199:Trehalose
keywords: Active wound dressings; Acute and chronic wounds; Skin regeneration; Wound irrigation