title: The use of hydrogen gas clearance for blood flow measurements in single endogenous and transplanted pancreatic islets.
abstract: The blood perfusion of pancreatic islets is regulated independently from that of the exocrine pancreas, and is of importance for multiple aspects of normal islet function, and probably also during impaired glucose tolerance. Single islet blood flow has been difficult to evaluate due to technical limitations. We therefore adapted a hydrogen gas washout technique using microelectrodes to allow such measurements. Platinum micro-electrodes monitored hydrogen gas clearance from individual endogenous and transplanted islets in the pancreas of male Lewis rats and in human and mouse islets implanted under the renal capsule of male athymic mice. Both in the rat endogenous pancreatic islets as well as in the intra-pancreatically transplanted islets, the vascular conductance and blood flow values displayed a highly heterogeneous distribution, varying by factors 6-10 within the same pancreas. The blood flow of human and mouse islet grafts transplanted in athymic mice was approximately 30% lower than that in the surrounding renal parenchyma. The present technique provides unique opportunities to study the islet vascular dysfunction seen after transplantation, but also allows for investigating the effects of genetic and environmental perturbations on islet blood flow at the single islet level in vivo.
authors.affiliation: Department of Medical Cell Biology, Uppsala University, Sweden; Department of Immunology, Genetics and Pathology, Uppsala University, Sweden. Electronic address: Andreea.barbu@igp.uu.se.
authors.forename: Andreea
authors.initials: A
authors.lastname: Barbu
authors.affiliation: Department of Medical Cell Biology, Uppsala University, Sweden. Electronic address: Leif.jansson@mcb.uu.se.
authors.forename: Leif
authors.initials: L
authors.lastname: Jansson
authors.affiliation: Department of Medical Cell Biology, Uppsala University, Sweden. Electronic address: Monica.sandberg@mcb.uu.se.
authors.forename: Monica
authors.initials: M
authors.lastname: Sandberg
authors.affiliation: Department of Medical Cell Biology, Uppsala University, Sweden. Electronic address: My.quach@mcb.uu.se.
authors.forename: My
authors.initials: M
authors.lastname: Quach
authors.affiliation: Department of Medical Cell Biology, Uppsala University, Sweden; Department of Medical and Health Sciences, University of Linköping, Sweden. Electronic address: Fredrik.palm@mcb.uu.se.
authors.forename: Fredrik
authors.initials: F
authors.lastname: Palm
mesh_terms: D000818:Animals; D001783:Blood Flow Velocity; D004867:Equipment Design; D005240:Feasibility Studies; D005740:Gases; D064593:Heterografts; D006801:Humans; D006859:Hydrogen / Q000097:blood; D007515:Islets of Langerhans / Q000098:blood supply* / Q000601:surgery*; D016381:Islets of Langerhans Transplantation; D017078:Laser-Doppler Flowmetry; D008297:Male; D008810:Mice, Inbred C57BL; D008819:Mice, Nude; D008839:Microelectrodes; D008863:Microspheres; D011237:Predictive Value of Tests; D011917:Rats, Inbred Lew; D012039:Regional Blood Flow; D012212:Rheology / Q000295:instrumentation / Q000379:methods*; D013997:Time Factors
chemical_list: D005740:Gases; D006859:Hydrogen
keywords: Blood flow; Hydrogen gas washout; In vivo; Pancreatic islets; Transplanted islets