title: Antihypertensive effect of the bovine casein-derived peptide Met-Lys-Pro.
abstract: The antihypertensive effect of the bovine casein-derived peptide Met-Lys-Pro (MKP) was examined in vitro and in vivo. MKP showed angiotensin I-converting enzyme (ACE)-inhibitory activity in vitro (IC50 = 0.43 μM). An in vivo kinetics study using radiolabeled Met-[1-(14)C]Lys-Pro ((14)C-MKP) showed that orally administered (14)C-MKP to spontaneously hypertensive rats (SHRs) was absorbed and moved into the plasma. In vitro vasoconstriction of thoracic aorta preparations, which was induced by adding angiotensin I, was reduced by prior exposure of MKP. A single oral dose of MKP lowered systolic blood pressure (SBP) of SHRs, and repeated oral administration of MKP for 28 days significantly lowered SBP of SHRs. The results obtained in the present study suggest that orally administrated MKP can be absorbed into the plasma and its ACE-inhibitory activity may contribute to induce the antihypertensive effect in vivo.
authors.affiliation: Morinaga Milk Industry Co., Ltd, Functional Food Research Department, Food Science & Technology Institute, 1-83, 5-Chome, Higashihara, Zama-City, Kanagawa-Pref. 252-8583, Japan. Electronic address: a_yamada@morinagamilk.co.jp.
authors.forename: Akio
authors.initials: A
authors.lastname: Yamada
authors.affiliation: Morinaga Milk Industry Co., Ltd, Functional Food Research Department, Food Science & Technology Institute, 1-83, 5-Chome, Higashihara, Zama-City, Kanagawa-Pref. 252-8583, Japan.
authors.forename: Takuma
authors.initials: T
authors.lastname: Sakurai
authors.affiliation: Morinaga Milk Industry Co., Ltd, Functional Food Research Department, Food Science & Technology Institute, 1-83, 5-Chome, Higashihara, Zama-City, Kanagawa-Pref. 252-8583, Japan.
authors.forename: Daisuke
authors.initials: D
authors.lastname: Ochi
authors.affiliation: Morinaga Milk Industry Co., Ltd, Functional Food Research Department, Food Science & Technology Institute, 1-83, 5-Chome, Higashihara, Zama-City, Kanagawa-Pref. 252-8583, Japan.
authors.forename: Eri
authors.initials: E
authors.lastname: Mitsuyama
authors.affiliation: Morinaga Milk Industry Co., Ltd, Functional Food Research Department, Food Science & Technology Institute, 1-83, 5-Chome, Higashihara, Zama-City, Kanagawa-Pref. 252-8583, Japan.
authors.forename: Koji
authors.initials: K
authors.lastname: Yamauchi
authors.affiliation: Morinaga Milk Industry Co., Ltd, Functional Food Research Department, Food Science & Technology Institute, 1-83, 5-Chome, Higashihara, Zama-City, Kanagawa-Pref. 252-8583, Japan.
authors.forename: Fumiaki
authors.initials: F
authors.lastname: Abe
mesh_terms: D000284:Administration, Oral; D000806:Angiotensin-Converting Enzyme Inhibitors / Q000737:chemistry / Q000494:pharmacology; D000818:Animals; D000959:Antihypertensive Agents / Q000494:pharmacology*; D001794:Blood Pressure / Q000187:drug effects; D002364:Caseins / Q000737:chemistry / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D008297:Male; D010455:Peptides / Q000737:chemistry*; D051381:Rats; D011918:Rats, Inbred SHR; D017208:Rats, Wistar; D014661:Vasoconstriction / Q000187:drug effects
chemical_list: D000806:Angiotensin-Converting Enzyme Inhibitors; D000959:Antihypertensive Agents; D002364:Caseins; D010455:Peptides
keywords: (14)C-labeled peptide; ACE-inhibitory peptide; Angiotensin I-converting enzyme; Bioavailability; Casein; Hypertension; Ile-Pro-Pro (CID 24978506); Val-Pro-Pro (CID 9818200); Val-Tyr (CID 4065033)