title: Transcriptional analysis of porcine circovirus-like virus P1.
abstract: BACKGROUND
  Recently identified porcine circovirus-like virus P1 has the smallest DNA viral genome. In this study, we identified the viral genes and their corresponding mRNA transcripts.
  
  
  RESULTS
  The RNAs of P1, synthesized in porcine kidney cells, were examined with northern blotting and PCR analyses. Eight virus-specific RNAs were detected. Four mRNAs (open reading frames (ORFs) 1, 2, 4, and 5) are encoded by the viral (-) strand and four (ORFs 3, 6, 7, and 8) are encoded by the viral (+) strand. All proteins encoded by the ORFs of the P1 virus are less than 50 amino acids in length, except that encoded by ORF1 (113 amino acids).
  
  
  CONCLUSIONS
  We show a very complex viral transcription pattern in P1-infected cells.
authors.affiliation: Jiangsu Academy of Agricultural Sciences · Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture · National Center for Engineering Research of Veterinary Bio-products, Institute of Veterinary Medicine, Nanjing, 210014, China. wlbwh@hotmail.com.
authors.forename: Li-bin
authors.initials: LB
authors.lastname: Wen
authors.affiliation: Jiangsu Academy of Agricultural Sciences · Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture · National Center for Engineering Research of Veterinary Bio-products, Institute of Veterinary Medicine, Nanjing, 210014, China. wangfengzhi@126.com.
authors.forename: Feng-zhi
authors.initials: FZ
authors.lastname: Wang
authors.affiliation: Jiangsu Academy of Agricultural Sciences · Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture · National Center for Engineering Research of Veterinary Bio-products, Institute of Veterinary Medicine, Nanjing, 210014, China. kwh2003@yeah.net.
authors.forename: Kong-wang
authors.initials: KW
authors.lastname: He
authors.affiliation: Jiangsu Academy of Agricultural Sciences · Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture · National Center for Engineering Research of Veterinary Bio-products, Institute of Veterinary Medicine, Nanjing, 210014, China. libinan@126.com.
authors.forename: Bin
authors.initials: B
authors.lastname: Li
authors.affiliation: Jiangsu Academy of Agricultural Sciences · Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture · National Center for Engineering Research of Veterinary Bio-products, Institute of Veterinary Medicine, Nanjing, 210014, China. wxm@gmail.com.
authors.forename: Xiao-min
authors.initials: XM
authors.lastname: Wang
authors.affiliation: Jiangsu Academy of Agricultural Sciences · Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture · National Center for Engineering Research of Veterinary Bio-products, Institute of Veterinary Medicine, Nanjing, 210014, China. guorl974@163.com.
authors.forename: Rong-li
authors.initials: RL
authors.lastname: Guo
authors.affiliation: Jiangsu Academy of Agricultural Sciences · Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture · National Center for Engineering Research of Veterinary Bio-products, Institute of Veterinary Medicine, Nanjing, 210014, China. xiejp1969@163.com.
authors.forename: Jian-ping
authors.initials: JP
authors.lastname: Xie
mesh_terms: D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern / Q000662:veterinary; D018173:Circoviridae Infections / Q000662:veterinary / Q000821:virology; D017925:Circovirus / Q000235:genetics*; D016679:Genome, Viral / Q000235:genetics; D008854:Microscopy, Electron; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012367:RNA, Viral / Q000235:genetics; D013552:Swine / Q000821:virology; D013553:Swine Diseases / Q000821:virology; D014771:Virion / Q000648:ultrastructure
chemical_list: D012367:RNA, Viral