title: OLED-based DNA biochip for Campylobacter spp. detection in poultry meat samples.
abstract: Integrated biochips are the ideal solution for producing portable diagnostic systems that uncouple diagnosis from centralized laboratories. These portable devices exploit a multi-disciplinary approach, are cost effective and have several advantages including broader accessibility, high sensitivity, quick test results and ease of use. The application of such a device in food safety is considered in this paper. Fluorescence detection of a specific biological probe excited by an optical source is one of the most commonly used methods for quantitative analysis on biochips. In this study, we designed and characterized a miniaturized, highly-sensitive DNA biochip based on a deep-blue organic light-emitting diode. The molecular design of the diode was optimized to excite a fluorophore-conjugated DNA probe and tested using real meat samples to obtain a high sensitivity and specificity against one of the most common poultry meat contaminants: Campylobacter spp. Real samples were analyzed also by classical plate methods and molecular methods to validate the results obtained by the new DNA-biochip. The high sensitivity obtained by the OLED based biochip (0.37ng/μl) and the short time required for the results (about 24h) indicate the usefulness of the system.
authors.affiliation: Department of Food Science, University of Udine, via Sondrio 2/A, 33100 Udine, Italy. Electronic address: marisa.manzano@uniud.it.
authors.forename: Marisa
authors.initials: M
authors.lastname: Manzano
authors.affiliation: Department of Food Science, University of Udine, via Sondrio 2/A, 33100 Udine, Italy.
authors.forename: Francesca
authors.initials: F
authors.lastname: Cecchini
authors.affiliation: Department of Food Science, University of Udine, via Sondrio 2/A, 33100 Udine, Italy.
authors.forename: Marco
authors.initials: M
authors.lastname: Fontanot
authors.affiliation: Department of Food Science, University of Udine, via Sondrio 2/A, 33100 Udine, Italy.
authors.forename: Lucilla
authors.initials: L
authors.lastname: Iacumin
authors.affiliation: Department of Food Science, University of Udine, via Sondrio 2/A, 33100 Udine, Italy.
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Comi
authors.affiliation: Université de Toulouse, UPS, INPT, CNRS-LAPLACE, 118 Route de Narbonne, 31062 Toulouse, Cedex 9, France; OR-EL.doo, Volariceva Ulica 6, 5222 Kobarid, Slovenija.
authors.forename: Patrizia
authors.initials: P
authors.lastname: Melpignano
mesh_terms: D000818:Animals; D015374:Biosensing Techniques / Q000379:methods*; D002167:Campylobacter / Q000235:genetics / Q000302:isolation & purification*; D004247:DNA / Q000235:genetics / Q000302:isolation & purification*; D005456:Fluorescent Dyes; D005516:Food Microbiology; D006801:Humans; D008460:Meat / Q000382:microbiology; D011200:Poultry / Q000382:microbiology
chemical_list: D005456:Fluorescent Dyes; D004247:DNA
keywords: Campylobacter spp.; DNA biochip; Organic light emitting diode (OLED); Poultry meat