title: A nairovirus isolated from African bats causes haemorrhagic gastroenteritis and severe hepatic disease in mice.
abstract: Bats can carry important zoonotic pathogens. Here we use a combination of next-generation sequencing and classical virus isolation methods to identify novel nairoviruses from bats captured from a cave in Zambia. This nairovirus infection is highly prevalent among giant leaf-nosed bats, Hipposideros gigas (detected in samples from 16 individuals out of 38). Whole-genome analysis of three viral isolates (11SB17, 11SB19 and 11SB23) reveals a typical bunyavirus tri-segmented genome. The strains form a single phylogenetic clade that is divergent from other known nairoviruses, and are hereafter designated as Leopards Hill virus (LPHV). When i.p. injected into mice, the 11SB17 strain causes only slight body weight loss, whereas 11SB23 produces acute and lethal disease closely resembling that observed with Crimean-Congo Haemorrhagic Fever virus in humans. We believe that our LPHV mouse model will be useful for research on the pathogenesis of nairoviral haemorrhagic disease.
authors.affiliation: 1] Hokudai Center for Zoonosis Control in Zambia, Research Center for Zoonosis Control, Hokkaido University, N20, W10, Kita-ku, Sapporo 001-0020, Japan [2] Department of Disease Control, School of Veterinary Medicine, University of Zambia, Great East Road, PO Box 32379, Lusaka 10101, Zambia.
authors.forename: Akihiro
authors.initials: A
authors.lastname: Ishii
authors.affiliation: Division of Bioinformatics, Research Center for Zoonosis Control, Hokkaido University, Sapporo 001-0020, Japan.
authors.forename: Keisuke
authors.initials: K
authors.lastname: Ueno
authors.affiliation: Division of Molecular Pathobiology, Research Center for Zoonosis Control, Hokkaido University, Sapporo 001-0020, Japan.
authors.forename: Yasuko
authors.initials: Y
authors.lastname: Orba
authors.affiliation: Division of Molecular Pathobiology, Research Center for Zoonosis Control, Hokkaido University, Sapporo 001-0020, Japan.
authors.forename: Michihito
authors.initials: M
authors.lastname: Sasaki
authors.affiliation: Department of Para-clinical studies, School of Veterinary Medicine, University of Zambia, Great East Road, PO Box 32379, Lusaka 10101, Zambia.
authors.forename: Ladslav
authors.initials: L
authors.lastname: Moonga
authors.affiliation: Department of Para-clinical studies, School of Veterinary Medicine, University of Zambia, Great East Road, PO Box 32379, Lusaka 10101, Zambia.
authors.forename: Bernard M
authors.initials: BM
authors.lastname: Hang'ombe
authors.affiliation: Department of Disease Control, School of Veterinary Medicine, University of Zambia, Great East Road, PO Box 32379, Lusaka 10101, Zambia.
authors.forename: Aaron S
authors.initials: AS
authors.lastname: Mweene
authors.affiliation: Department of Comparative Pathology, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 001-0020, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Umemura
authors.affiliation: Division of Bioinformatics, Research Center for Zoonosis Control, Hokkaido University, Sapporo 001-0020, Japan.
authors.forename: Kimihito
authors.initials: K
authors.lastname: Ito
authors.affiliation: 1] Centre for Research in Infectious Diseases, University College Dublin, Dublin 4, Ireland [2] Global Virus Network, Baltimore, Maryland 21201, USA [3] Global Institution for Collaborative Research and Education (GI-CoRE), Hokkaido University, Sapporo 001-0020, Japan.
authors.forename: William W
authors.initials: WW
authors.lastname: Hall
authors.affiliation: 1] Department of Disease Control, School of Veterinary Medicine, University of Zambia, Great East Road, PO Box 32379, Lusaka 10101, Zambia [2] Division of Molecular Pathobiology, Research Center for Zoonosis Control, Hokkaido University, Sapporo 001-0020, Japan [3] Global Virus Network, Baltimore, Maryland 21201, USA [4] Global Institution for Collaborative Research and Education (GI-CoRE), Hokkaido University, Sapporo 001-0020, Japan.
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Sawa
mesh_terms: D000818:Animals; D001483:Base Sequence; D002685:Chiroptera / Q000821:virology*; D004195:Disease Models, Animal; D005759:Gastroenteritis / Q000821:virology*; D006471:Gastrointestinal Hemorrhage / Q000821:virology*; D006479:Hemorrhagic Fever, Crimean / Q000821:virology*; D006524:Hepatitis, Viral, Animal / Q000821:virology*; D051379:Mice; D008969:Molecular Sequence Data; D016855:Nairovirus / Q000235:genetics* / Q000302:isolation & purification; D012367:RNA, Viral / Q000032:analysis*
chemical_list: D012367:RNA, Viral