title: Response surface optimization of ultrasound assisted extraction of pectin from pomegranate peel.
abstract: Ultrasound assisted extraction of pectin from waste pomegranate peel was investigated and optimized using Box-Behnken response surface design coupled with numerical optimization technique. The individual and interactive effect of process variables (solid-liquid ratio, pH, extraction time and temperature) on the pectin yield was studied. The experimental data obtained were analyzed by Pareto analysis of variance (ANOVA) and second-order polynomial models were developed using multiple regression analysis. The models developed from the experimental design were predictive and good fit with the experimental data with high coefficient of determination (R(2)) value. The optimal extraction condition was found to be 1:17.52 g/ml of solid-liquid ratio, 1.27 of pH, 28.31 min of extraction time and 61.90 °C of extraction temperature respectively. Under the optimal conditions, experimental yield was very close to the predicted values.
authors.affiliation: Department of Biotechnology, Kamaraj College of Engineering and Technology, Virudhunagar 626 001, Tamil Nadu, India.
authors.forename: I Ganesh
authors.initials: IG
authors.lastname: Moorthy
authors.affiliation: Department of Food Technology, Kongu Engineering College, Perundurai 638 052, Erode, Tamil Nadu, India. Electronic address: prakashmaran@gmail.com.
authors.forename: J Prakash
authors.initials: JP
authors.lastname: Maran
authors.affiliation: Department of Biotechnology, Kamaraj College of Engineering and Technology, Virudhunagar 626 001, Tamil Nadu, India.
authors.forename: S Muneeswari
authors.initials: SM
authors.lastname: Surya
authors.affiliation: Department of Biotechnology, Kamaraj College of Engineering and Technology, Virudhunagar 626 001, Tamil Nadu, India.
authors.forename: S
authors.initials: S
authors.lastname: Naganyashree
authors.affiliation: Department of Biotechnology, Kamaraj College of Engineering and Technology, Virudhunagar 626 001, Tamil Nadu, India.
authors.forename: C S
authors.initials: CS
authors.lastname: Shivamathi
mesh_terms: D000704:Analysis of Variance; D029561:Lythraceae / Q000737:chemistry*; D008962:Models, Theoretical; D010368:Pectins / Q000302:isolation & purification*; D012044:Regression Analysis; D015203:Reproducibility of Results; D014465:Ultrasonics / Q000379:methods*; D014866:Waste Products / Q000032:analysis*
chemical_list: D014866:Waste Products; D010368:Pectins
keywords: Extraction; Pectin; Pomegranate peel