title: MicroRNA-383 inhibits anchorage-independent growth and induces cell cycle arrest of glioma cells by targeting CCND1.
abstract: In recent years, microRNAs (miRNAs) have been proved to be closely related to the tumorigenesis and progression. An increasing number of researches have shown that microRNAs function as oncogenes or tumor suppressor genes in human malignant tumors. This study aims to explore the effects of microRNA-383 (miR-383) on malignant biological function of human gliomas. We detected the expression of miR-383 in glioma tissues and normal brain tissues by quantitative real-time PCR. Anchorage-independent growth assays, and flow cytometry were used to evaluate the functions of miR-383 that involves in cell growth and cell cycle. Western blotting assay was used to examine protein expression levels of Cyclin D1 (CCND1), a cell cycle-associated oncogene which has a predicted binding site of miR-383 within its 3'-untranslated region (3'-UTR), and luciferase activity assay was used to evaluate the 3'-UTR activity of CCND1. In this study, we found that miR-383 expression level was lower in gliomas than normal brain tissues. Overexpression of miR-383 in U251 and U87 cells showed a significant inhibitory effect on cell growth, which accompanied with cell cycle G0/G1 arrest as well as downregulation of CCND1 expression. Moreover, CCND1 was verified to be one of the direct targets of miR-383. In summary, this study suggested that miR-383 plays the role of tumor suppressor by targeting CCND1 in glioma cells, and may be useful for developing a new therapeutic strategy for gliomas.
authors.affiliation: Department of Neurosurgery, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei, China. Electronic address: drxu2000@sina.com.
authors.forename: Zhou
authors.initials: Z
authors.lastname: Xu
authors.affiliation: The Central Hospital of Wuhan, Wuhan 430014, Hubei, China.
authors.forename: Xingruo
authors.initials: X
authors.lastname: Zeng
authors.affiliation: Department of Neurosurgery, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei, China.
authors.forename: Daofeng
authors.initials: D
authors.lastname: Tian
authors.affiliation: Department of Neurosurgery, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei, China.
authors.forename: Haitao
authors.initials: H
authors.lastname: Xu
authors.affiliation: Department of Neurosurgery, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Cai
authors.affiliation: Department of Neurosurgery, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei, China.
authors.forename: Junmin
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Neurosurgery, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei, China. Electronic address: chenqx666@126.com.
authors.forename: Qianxue
authors.initials: Q
authors.lastname: Chen
mesh_terms: D017209:Apoptosis / Q000235:genetics*; D002448:Cell Adhesion / Q000235:genetics*; D059447:Cell Cycle Checkpoints / Q000235:genetics*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics; D019938:Cyclin D1 / Q000235:genetics*; D005910:Glioma / Q000235:genetics* / Q000473:pathology*; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*
chemical_list: C530104:CCND1 protein, human; C558685:MIRN383 microRNA, human; D035683:MicroRNAs; D019938:Cyclin D1
keywords: Anchorage-independent growth; CCND1; Cell cycle; Glioma; MicroRNA-383