title: Enzyme-functionalized gold-coated magnetite nanoparticles as novel hybrid nanomaterials: synthesis, purification and control of enzyme function by low-frequency magnetic field.
abstract: The possibility of remotely inducing a defined effect on NPs by means of electromagnetic radiation appears attractive. From a practical point of view, this effect opens horizons for remote control of drug release systems, as well as modulation of biochemical functions in cells. Gold-coated magnetite nanoparticles are perfect candidates for such application. Herein, we have successfully synthesized core-shell NPs having magnetite cores and gold shells modified with various sulphur containing ligands and developed a new, simple and robust procedure for the purification of the resulting nanoparticles. The carboxylic groups displayed at the surface of the NPs were utilized for NP conjugation with a model enzyme (ChT). In the present study, we report the effect of the low-frequency AC magnetic field on the catalytic activity of the immobilized ChT. We show that the enzyme activity decreases upon exposure of the NPs to the field.
authors.affiliation: Laboratory of Chemical Design of Bionanomaterials, Chemistry Department, M.V. Lomonosov Moscow State University, Russian Federation; National University of Science and Technology MISiS, Leninsky Ave, 4, 119049 Moscow, Russian Federation. Electronic address: majouga@org.chem.msu.ru.
authors.forename: Alexander
authors.initials: A
authors.lastname: Majouga
authors.affiliation: Center for Nanotechnology in Drug Delivery and Division of Molecular Pharmaceutics, Eshelman School of Pharmacy, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.
authors.forename: Marina
authors.initials: M
authors.lastname: Sokolsky-Papkov
authors.affiliation: Laboratory of Chemical Design of Bionanomaterials, Chemistry Department, M.V. Lomonosov Moscow State University, Russian Federation.
authors.forename: Artem
authors.initials: A
authors.lastname: Kuznetsov
authors.affiliation: Laboratory of Chemical Design of Bionanomaterials, Chemistry Department, M.V. Lomonosov Moscow State University, Russian Federation.
authors.forename: Dmitry
authors.initials: D
authors.lastname: Lebedev
authors.affiliation: Laboratory of Chemical Design of Bionanomaterials, Chemistry Department, M.V. Lomonosov Moscow State University, Russian Federation.
authors.forename: Maria
authors.initials: M
authors.lastname: Efremova
authors.affiliation: Laboratory of Chemical Design of Bionanomaterials, Chemistry Department, M.V. Lomonosov Moscow State University, Russian Federation.
authors.forename: Elena
authors.initials: E
authors.lastname: Beloglazkina
authors.affiliation: Laboratory of Chemical Design of Bionanomaterials, Chemistry Department, M.V. Lomonosov Moscow State University, Russian Federation.
authors.forename: Polina
authors.initials: P
authors.lastname: Rudakovskaya
authors.affiliation: Laboratory of Chemical Design of Bionanomaterials, Chemistry Department, M.V. Lomonosov Moscow State University, Russian Federation.
authors.forename: Maxim
authors.initials: M
authors.lastname: Veselov
authors.affiliation: Laboratory of Chemical Design of Bionanomaterials, Chemistry Department, M.V. Lomonosov Moscow State University, Russian Federation.
authors.forename: Nikolay
authors.initials: N
authors.lastname: Zyk
authors.affiliation: Laboratory of Chemical Design of Bionanomaterials, Chemistry Department, M.V. Lomonosov Moscow State University, Russian Federation; R.G. Derzhavin Tambov State University, Russian Federation.
authors.forename: Yuri
authors.initials: Y
authors.lastname: Golovin
authors.affiliation: Laboratory of Chemical Design of Bionanomaterials, Chemistry Department, M.V. Lomonosov Moscow State University, Russian Federation; Center for Nanotechnology in Drug Delivery and Division of Molecular Pharmaceutics, Eshelman School of Pharmacy, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.
authors.forename: Natalia
authors.initials: N
authors.lastname: Klyachko
authors.affiliation: Laboratory of Chemical Design of Bionanomaterials, Chemistry Department, M.V. Lomonosov Moscow State University, Russian Federation; Center for Nanotechnology in Drug Delivery and Division of Molecular Pharmaceutics, Eshelman School of Pharmacy, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.
authors.forename: Alexander
authors.initials: A
authors.lastname: Kabanov
mesh_terms: D002850:Chromatography, Gel; D002918:Chymotrypsin / Q000737:chemistry*; D019343:Citric Acid / Q000737:chemistry; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D003545:Cysteine / Q000737:chemistry; D057075:Enzyme Assays; D004800:Enzymes, Immobilized / Q000737:chemistry*; D052203:Ferrosoferric Oxide / Q000737:chemistry; D006046:Gold / Q000737:chemistry*; D007700:Kinetics; D060526:Magnetic Fields; D058185:Magnetite Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D013499:Surface Properties
chemical_list: D020099:Coated Materials, Biocompatible; D004800:Enzymes, Immobilized; D058185:Magnetite Nanoparticles; D019343:Citric Acid; D006046:Gold; D002918:Chymotrypsin; C117608:alpha-chymotrypsin; D003545:Cysteine; D052203:Ferrosoferric Oxide
keywords: Enzyme catalytic activity inhibition; Enzyme immobilization; Gold-coated magnetite nanoparticles; Purification; Super low-frequency non-heating magnetic field