title: Novel cell-based odorant sensor elements based on insect odorant receptors.
abstract: Development of cell-based odorant sensor elements combined not only high degree of sensitivity and selectivity but also long-term stability is crucial for their practical applications. Here we report the development of a novel cell-based odorant sensor element that sensitively and selectively detects odorants and displays increased fluorescent intensities over a long period of time. Our odorant sensor elements, based on Sf21 cell lines expressing insect odorant receptors, are sensitive to the level of several tens of parts per billion in solution, can selectively distinguish between different types of odorants based on the odorant selectivity intrinsic to the expressed receptors, and have response times of approximately 13s. Specifically, with the use of Sf21 cells and insect odorant receptors, we demonstrated that the established cell lines stably expressing insect odorant receptors are able to detect odorants with consistent responsiveness for at least 2 months, thus exceeding the short life-span normally associated with cell-based sensors. We also demonstrated the development of a compact odorant sensor chip by integrating the established insect cell lines into a microfluidic chip. The methodology we established in this study, in conjunction with the large repertoire of insect odorant receptors, will aid in the development of practical cell-based odorant sensors for various applications, including food administration and health management.
authors.affiliation: Research Center for Advanced Science and Technology, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8904, Japan.
authors.forename: Hidefumi
authors.initials: H
authors.lastname: Mitsuno
authors.affiliation: Research Center for Advanced Science and Technology, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8904, Japan.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Sakurai
authors.affiliation: Janelia Farm Research Campus, Howard Hughes Medical Institute, 19700 Helix Dr Ashbum, VA 20147, USA.
authors.forename: Shigehiro
authors.initials: S
authors.lastname: Namiki
authors.affiliation: Graduate School of Information Science and Technology, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Mitsuhashi
authors.affiliation: Research Center for Advanced Science and Technology, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8904, Japan; Graduate School of Information Science and Technology, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan. Electronic address: kanzaki@rcast.u-tokyo.ac.jp.
authors.forename: Ryohei
authors.initials: R
authors.lastname: Kanzaki
mesh_terms: D000466:Alkadienes / Q000032:analysis*; D000818:Animals; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D002118:Calcium / Q000032:analysis; D002460:Cell Line; D004867:Equipment Design; D005233:Fatty Alcohols / Q000032:analysis*; D015870:Gene Expression; D006801:Humans; D019476:Insect Proteins / Q000235:genetics / Q000378:metabolism*; D007313:Insecta / Q000166:cytology* / Q000235:genetics / Q000378:metabolism; D056656:Lab-On-A-Chip Devices; D009812:Odorants / Q000032:analysis*; D018035:Receptors, Odorant / Q000235:genetics / Q000378:metabolism*
chemical_list: D000466:Alkadienes; D005233:Fatty Alcohols; D019476:Insect Proteins; D018035:Receptors, Odorant; C066482:bombykal; C006787:bombykol; D002118:Calcium
keywords: Calcium imaging; Insect odorant receptor; Odorant sensor element; Sf21 cell line