title: Phage display as a novel promising antivenom therapy: a review.
abstract: In this work, we present recent advances in the use of phage display technology for the preparation of antivenoms for animal toxin neutralization. Even though classical antivenoms have been used since the early 20th century, envenomation remains a global public health problem. Recently, the phage display technique has been used in an attempt to circumvent some of the difficulties associated with traditional preparations of antivenom. Here, we review studies that developed antibody fragments with potential inhibitory effects against animal toxins and discuss the most current technical issues and perspectives regarding phage display technology in this field.
authors.affiliation: Department of Biochemistry and Immunology, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, São Paulo, Brazil.
authors.forename: Eduardo Crosara
authors.initials: EC
authors.lastname: Roncolato
authors.affiliation: Department of Biochemistry and Immunology, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, São Paulo, Brazil.
authors.forename: Lucas Benício
authors.initials: LB
authors.lastname: Campos
authors.affiliation: Department of Biochemistry and Immunology, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, São Paulo, Brazil.
authors.forename: Gabriela
authors.initials: G
authors.lastname: Pessenda
authors.affiliation: Department of Biochemistry and Immunology, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, São Paulo, Brazil.
authors.forename: Luciano
authors.initials: L
authors.lastname: Costa e Silva
authors.affiliation: Department of Biochemistry and Immunology, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, São Paulo, Brazil.
authors.forename: Gilvan Pessoa
authors.initials: GP
authors.lastname: Furtado
authors.affiliation: Department of Biochemistry and Immunology, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, São Paulo, Brazil. Electronic address: jebarbos@fmrp.usp.br.
authors.forename: José Elpidio
authors.initials: JE
authors.lastname: Barbosa
mesh_terms: D057134:Antibodies, Neutralizing / Q000627:therapeutic use*; D000997:Antivenins / Q000627:therapeutic use*; D061505:Cell Surface Display Techniques / Q000379:methods*; D006801:Humans; D018448:Models, Immunological; D012909:Snake Bites / Q000188:drug therapy*
chemical_list: D057134:Antibodies, Neutralizing; D000997:Antivenins
keywords: Animal toxins; Antibodies; Antivenoms; Envenomation; New therapies; Phage display