title: TCR-mediated functions are enhanced in activated peripheral blood T cells isolated from leucocyte reduction systems.
abstract: Buffy coats are the most common method for the acquisition of activated primary human T cells for research or clinical applications, but recently leukocyte reduction system (LRS) cones have emerged as a viable source for these cells. In this study, we determined if activated human T cells derived from buffy coats or LRS cones had different functionality. No changes in the expression of surface receptors were observed except for a significant increase in CD44 expression on T cells isolated from LRS cones. LRS cone-derived T cells trended towards higher receptor-mediated cytokine production and had significantly increased donor-to-donor variability in IFN-γ production. TCR-induced ERK1/ERK2 and AKT phosphorylation was also increased in T cells isolated from LRS cones. In conclusion, LRS cones are an excellent source of T cells for clinical and research applications, but these cells have subtle functional differences from T cells isolated using standard buffy coats.
authors.affiliation: Department of Microbiology, Carver College of Medicine, University of Iowa, Iowa City, IA 52242, United States.
authors.forename: Mikaela M
authors.initials: MM
authors.lastname: Tremblay
authors.affiliation: Department of Microbiology, Carver College of Medicine, University of Iowa, Iowa City, IA 52242, United States. Electronic address: jon-houtman@uiowa.edu.
authors.forename: Jon C D
authors.initials: JC
authors.lastname: Houtman
mesh_terms: D006801:Humans; D018960:Hyaluronan Receptors / Q000276:immunology; D007371:Interferon-gamma / Q000276:immunology; D007962:Leukocytes / Q000276:immunology*; D008213:Lymphocyte Activation / Q000276:immunology*; D020935:MAP Kinase Signaling System / Q000276:immunology; D010766:Phosphorylation / Q000276:immunology; D051057:Proto-Oncogene Proteins c-akt / Q000276:immunology; D011948:Receptors, Antigen, T-Cell / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D018960:Hyaluronan Receptors; D011948:Receptors, Antigen, T-Cell; D007371:Interferon-gamma; D051057:Proto-Oncogene Proteins c-akt
keywords: Buffy coat; Leukocyte reduction systems; T cell receptor; T cells