title: Biohydrogen production from a novel alkalophilic isolate Clostridium sp. IODB-O3.
abstract: Hydrogen producing bacteria IODB-O3 was isolated from sludge and identified as Clostridium sp. by 16S rDNA gene analysis. In this study, biohydrogen production process was developed using low-cost agro-waste. Maximum H2 was produced at 37°C and pH 8.5. Maximum H2 yield was obtained 2.54±0.2mol-H2/mol-reducing sugar from wheat straw pre-hydrolysate (WSPH) and 2.61±0.1mol-H2/mol-reducing sugar from pre-treated wheat straw enzymatic-hydrolysate (WSEH). The cumulative H2 production (ml/L), 3680±105 and 3270±100, H2 production rate (ml/L/h), 153±5 and 136±5, and specific H2 production (ml/g/h), 511±5 and 681±10 with WSPH and WSEH were obtained, respectively. Biomass pre-treatment via steam-explosion generates ample amount of WSPH which remains unutilized for bioethanol production due to non-availability of efficient C5-fermenting microorganisms. This study shows that Clostridium sp. IODB-O3 is capable of utilizing WSPH efficiently for biohydrogen production. This would lead to reduced economic constrain on the overall cellulosic ethanol process and also establish a sustainable biohydrogen production process.
authors.affiliation: DBT-IOC Centre for Advanced Bio-Energy Research, Indian Oil Corporation Ltd, R&D Centre, Sector-13, Faridabad 121007, India. Electronic address: anilkpatel22@gmail.com.
authors.forename: Anil Kumar
authors.initials: AK
authors.lastname: Patel
authors.affiliation: DBT-IOC Centre for Advanced Bio-Energy Research, Indian Oil Corporation Ltd, R&D Centre, Sector-13, Faridabad 121007, India.
authors.forename: Arundhati
authors.initials: A
authors.lastname: Debroy
authors.affiliation: DBT-IOC Centre for Advanced Bio-Energy Research, Indian Oil Corporation Ltd, R&D Centre, Sector-13, Faridabad 121007, India.
authors.forename: Sandeep
authors.initials: S
authors.lastname: Sharma
authors.affiliation: DBT-IOC Centre for Advanced Bio-Energy Research, Indian Oil Corporation Ltd, R&D Centre, Sector-13, Faridabad 121007, India.
authors.forename: Reetu
authors.initials: R
authors.lastname: Saini
authors.affiliation: DBT-IOC Centre for Advanced Bio-Energy Research, Indian Oil Corporation Ltd, R&D Centre, Sector-13, Faridabad 121007, India.
authors.forename: Anshu
authors.initials: A
authors.lastname: Mathur
authors.affiliation: DBT-IOC Centre for Advanced Bio-Energy Research, Indian Oil Corporation Ltd, R&D Centre, Sector-13, Faridabad 121007, India.
authors.forename: Ravi
authors.initials: R
authors.lastname: Gupta
authors.affiliation: DBT-IOC Centre for Advanced Bio-Energy Research, Indian Oil Corporation Ltd, R&D Centre, Sector-13, Faridabad 121007, India.
authors.forename: Deepak Kumar
authors.initials: DK
authors.lastname: Tuli
mesh_terms: D056804:Biofuels; D018533:Biomass; D019149:Bioreactors / Q000382:microbiology; D002244:Carbon / Q000378:metabolism*; D003013:Clostridium / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D000431:Ethanol / Q000737:chemistry; D005285:Fermentation; D006859:Hydrogen / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D008031:Lignin / Q000737:chemistry / Q000378:metabolism; D012722:Sewage / Q000382:microbiology; D013227:Steam; D013696:Temperature; D014908:Triticum / Q000737:chemistry
chemical_list: D056804:Biofuels; D012722:Sewage; D013227:Steam; C036909:lignocellulose; D000431:Ethanol; D002244:Carbon; D006859:Hydrogen; D008031:Lignin
keywords: Anaerobic fermentation; Biohydrogen; Clostridium sp.; Dark fermentation; Steam-explosion