title: Metabolic pathway balancing and its role in the production of biofuels and chemicals.
abstract: In the last decade, metabolic engineering benefited greatly from systems and synthetic biology due to substantial advancements in those fields. As a result, technologies and methods evolved to be more complex and controllable than ever. In this review, we highlight up-to-date case studies using these techniques, examine their potential, and stress their importance for production of compounds such as fatty acids, alcohols, and high value chemicals. Beginning with basic rational control techniques and continuing with advanced level modern approaches, we review the vast number of possibilities for controlling metabolic fluxes. Our aim is to give a brief and informative insight about commonly used tools and universalized methodologies for metabolic pathway balancing and optimization.
authors.affiliation: Rensselaer Polytechnic Institute, Department of Chemical and Biological Engineering, Center for Biotechnology and Interdisciplinary Studies, Troy, NY, USA.
authors.forename: J Andrew
authors.initials: JA
authors.lastname: Jones
authors.affiliation: Rensselaer Polytechnic Institute, Department of Chemical and Biological Engineering, Center for Biotechnology and Interdisciplinary Studies, Troy, NY, USA.
authors.forename: Ö Duhan
authors.initials: ÖD
authors.lastname: Toparlak
authors.affiliation: Rensselaer Polytechnic Institute, Department of Chemical and Biological Engineering, Center for Biotechnology and Interdisciplinary Studies, Troy, NY, USA. Electronic address: koffam@rpi.edu.
authors.forename: Mattheos A G
authors.initials: MA
authors.lastname: Koffas
mesh_terms: D000438:Alcohols / Q000378:metabolism; D056804:Biofuels; D005227:Fatty Acids / Q000378:metabolism; D060847:Metabolic Engineering; D053858:Metabolic Networks and Pathways; D058615:Synthetic Biology
chemical_list: D000438:Alcohols; D056804:Biofuels; D005227:Fatty Acids