title: Decrease of PKB/Akt Phosphorylation is Partially Mediated by SAPK/JNK Activation in Serum-free L6 Myoblasts Starved with Low Glucose.
abstract: [Purpose] Studies have been using cell cultures of muscle cells to mimic atrophy in in vivo and in vitro tests. However, changes in the activation of atrophy-related PKB/Akt is not fully understood in serum-free starved skeletal muscle cells. The purpose of the present study was to determine the change of PKB/Akt phosphorylation in L6 myoblasts under serum-free starvation conditions. [Methods] We used western blotting to examine PKB/Akt expression and phosphorylation in atrophied L6 myoblasts. [Results] The phosphorylation of PKB/Akt was significantly lower in L6 myoblasts under serum-free starvation than that of the control group. Serum-free starvation for 6, 12, 24, 36, 48, 72, 96, and 120 hours significantly decreased the phosphorylation of PKB/Akt. Furthermore, the decrease of PKB/Akt phosphorylation under serum-free starvation was partially restored by SP600125, an inhibitor of SAPK/JNK. [Conclusion] These results suggest that decrease of PKB/Akt phosphorylation due to serum-free starvation with low glucose is partially related to the activity of SAPK/JNK in L6 myoblasts.
authors.affiliation: Laboratory of Health Science and Nanophysiotherapy, Department of Physical Therapy, Graduate School, Yongin University, Republic of Korea ; Laboratory of Health Science and Nanophysiotherapy, Department of Physical Therapy, Graduate School, Yongin University, Republic of Korea.
authors.forename: Mee-Young
authors.initials: MY
authors.lastname: Kim
authors.affiliation: Laboratory of Health Science and Nanophysiotherapy, Department of Physical Therapy, Graduate School, Yongin University, Republic of Korea.
authors.forename: Jeong-Uk
authors.initials: JU
authors.lastname: Lee
authors.affiliation: Laboratory of Health Science and Nanophysiotherapy, Department of Physical Therapy, Graduate School, Yongin University, Republic of Korea.
authors.forename: Ju-Hyun
authors.initials: JH
authors.lastname: Kim
authors.affiliation: Laboratory of Health Science and Nanophysiotherapy, Department of Physical Therapy, Graduate School, Yongin University, Republic of Korea.
authors.forename: Lim-Kyu
authors.initials: LK
authors.lastname: Lee
authors.affiliation: Laboratory of Health Science and Nanophysiotherapy, Department of Physical Therapy, Graduate School, Yongin University, Republic of Korea.
authors.forename: Seung-Min
authors.initials: SM
authors.lastname: Yang
authors.affiliation: Laboratory of Health Science and Nanophysiotherapy, Department of Physical Therapy, Graduate School, Yongin University, Republic of Korea.
authors.forename: Byoung-Sun
authors.initials: BS
authors.lastname: Park
authors.affiliation: Laboratory of Health Science and Nanophysiotherapy, Department of Physical Therapy, Graduate School, Yongin University, Republic of Korea.
authors.forename: Hye-Joo
authors.initials: HJ
authors.lastname: Jeon
authors.affiliation: Laboratory of Health Science and Nanophysiotherapy, Department of Physical Therapy, Graduate School, Yongin University, Republic of Korea.
authors.forename: Won-Deok
authors.initials: WD
authors.lastname: Lee
authors.affiliation: Laboratory of Health Science and Nanophysiotherapy, Department of Physical Therapy, Graduate School, Yongin University, Republic of Korea.
authors.forename: Ji-Woong
authors.initials: JW
authors.lastname: Noh
authors.affiliation: Department of Taekwondo Instructor Education, College of Martial Arts, Yongin University, Republic of Korea.
authors.forename: Taek-Yong
authors.initials: TY
authors.lastname: Kwak
authors.affiliation: Department of Judo, College of Martial Arts, Yongin University, Republic of Korea.
authors.forename: Sung-Ho
authors.initials: SH
authors.lastname: Jang
authors.affiliation: Combative Martial Arts Training, College of Martial Arts, Yongin University, Republic of Korea.
authors.forename: Tae-Hyun
authors.initials: TH
authors.lastname: Lee
authors.affiliation: Combative Martial Arts Training, College of Martial Arts, Yongin University, Republic of Korea.
authors.forename: Ju-Young
authors.initials: JY
authors.lastname: Kim
authors.affiliation: Institute of Functional Genomics, Department of Physiology, School of Medicine, Konkuk University, Republic of Korea.
authors.forename: Bokyung
authors.initials: B
authors.lastname: Kim
authors.affiliation: Department of Physical Therapy, College of Public Health and Welfare, Yongin University: Yongin 449-714, Republic of Korea.
authors.forename: Junghwan
authors.initials: J
authors.lastname: Kim
keywords: L6 myoblasts; PKB/Akt; Serum-free starvation