title: Anti-loxoscelic horse serum produced against a recombinant dermonecrotic protein of Brazilian Loxosceles intermedia spider neutralize lethal effects of Loxosceles laeta venom from Peru.
abstract: In this work, an anti-loxoscelic serum was produced by immunizing horses with a recombinant dermonecrotic protein from Loxosceles intermedia (rLiD1). Anti-rLiD1 antibodies were able to recognize different species of Loxosceles venoms by Western Blot and ELISA. The efficacy of anti-rLiD1 serum against the toxic effects of Loxosceles laeta (Peru) venom was tested, showing that anti-rLiD1 serum can neutralize those effects. This study confirms that recombinant proteins can be good candidates to replace crude venoms for antivenom production.
authors.affiliation: Departamento de Bioquímica-Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte 31270-901, Minas Gerais, Brazil.
authors.forename: C G
authors.initials: CG
authors.lastname: Duarte
authors.affiliation: Instituto Nacional de Salud, Lima, Peru.
authors.forename: C
authors.initials: C
authors.lastname: Bonilla
authors.affiliation: Departamento de Bioquímica-Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte 31270-901, Minas Gerais, Brazil.
authors.forename: G
authors.initials: G
authors.lastname: Guimarães
authors.affiliation: Universidade do Extremo Sul Catarinense - UNESC, Criciúma, Santa Catarina, Brazil.
authors.forename: R A
authors.initials: RA
authors.lastname: Machado de Avila
authors.affiliation: Departamento de Bioquímica-Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte 31270-901, Minas Gerais, Brazil.
authors.forename: T M
authors.initials: TM
authors.lastname: Mendes
authors.affiliation: Instituto Nacional de Salud, Lima, Peru.
authors.forename: W
authors.initials: W
authors.lastname: Silva
authors.affiliation: Instituto Nacional de Salud, Lima, Peru.
authors.forename: B
authors.initials: B
authors.lastname: Tintaya
authors.affiliation: Universidad Nacional Mayor de San Marcos, Lima, Peru.
authors.forename: A
authors.initials: A
authors.lastname: Yarleque
authors.affiliation: Departamento de Bioquímica-Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte 31270-901, Minas Gerais, Brazil. Electronic address: olortegi@icb.ufmg.br.
authors.forename: C
authors.initials: C
authors.lastname: Chávez-Olórtegui
mesh_terms: D000818:Animals; D000997:Antivenins / Q000276:immunology* / Q000494:pharmacology; D015153:Blotting, Western; D001938:Brazil; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D006736:Horses; D009500:Neutralization Tests; D010568:Peru; D010727:Phosphoric Diester Hydrolases / Q000032:analysis / Q000737:chemistry* / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism*; D013045:Species Specificity; D013111:Spider Venoms / Q000737:chemistry* / Q000201:enzymology
chemical_list: D000997:Antivenins; D011994:Recombinant Proteins; D013111:Spider Venoms; C055161:loxosceles venom; D010727:Phosphoric Diester Hydrolases; C055160:sphingomyelin phosphodiesterase D
keywords: Antivenom; Immunization; Loxosceles; Neutralization; Recombinant protein