title: GC-FID coupled with chemometrics for quantitative and chemical fingerprinting analysis of Alpinia oxyphylla oil.
abstract: Analytical methods for quantitative analysis and chemical fingerprinting of volatile oils from Alpinia oxyphylla were established. The volatile oils were prepared by hydrodistillation, and the yields were between 0.82% and 1.33%. The developed gas chromatography-flame ionization detection (GC-FID) method showed good specificity, linearity, reproducibility, stability and recovery, and could be used satisfactorily for quantitative analysis. The results showed that the volatile oils contained 2.31-77.30 μL/mL p-cymene and 12.38-99.34 mg/mL nootkatone. A GC-FID fingerprinting method was established, and the profiles were analyzed using chemometrics. GC-MS was used to identify the principal compounds in the GC-FID profiles. The profiles of almost all the samples were consistent and stable. The harvesting time and source were major factors that affected the profile, while the volatile oil yield and the nootkatone content had minor secondary effects.
authors.affiliation: Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100193, China; Jilin Agricultural University, Changchun 130118, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Miao
authors.affiliation: Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100193, China.
authors.forename: Weijun
authors.initials: W
authors.lastname: Kong
authors.affiliation: Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100193, China; Hainan Branch Institute of Medicinal Plant Development, Chinese Academy of Medicinal Sciences & Peking Union Medical College, Wanning 571533, China.
authors.forename: Xiangsheng
authors.initials: X
authors.lastname: Zhao
authors.affiliation: Jilin Agricultural University, Changchun 130118, China.
authors.forename: Shihai
authors.initials: S
authors.lastname: Yang
authors.affiliation: Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100193, China; Hainan Branch Institute of Medicinal Plant Development, Chinese Academy of Medicinal Sciences & Peking Union Medical College, Wanning 571533, China. Electronic address: yangmeihua15@hotmail.com.
authors.forename: Meihua
authors.initials: M
authors.lastname: Yang
mesh_terms: D032429:Alpinia; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D002849:Chromatography, Gas / Q000379:methods; D005410:Flame Ionization / Q000379:methods; D009822:Oils, Volatile / Q000032:analysis* / Q000737:chemistry*
chemical_list: D009822:Oils, Volatile
keywords: Alpinia oxyphylla oil; Chemical fingerprint; Chemometrics; GC-FID; Quantitative analysis