title: Over-expression of TaEXPB23, a wheat expansin gene, improves oxidative stress tolerance in transgenic tobacco plants.
abstract: Expansins are cell wall proteins inducing cell wall loosening and participate in all plant growth and development processes which are associated with cell wall modifications. Here, TaEXPB23, a wheat expansin gene, was investigated and the tolerance to oxidative stress was strongly enhanced in over-expression tobacco plants. Our results revealed that over-expressing TaEXPB23 influenced the activity of antioxidant enzymes: in particular, the activity of the cell wall-bound peroxidase. The enhanced tolerance to oxidative stress and increased cell wall-bound peroxidase activity were partly inhibited by an anti-expansin antibody. The Arabidopsis expansin mutant atexpb2 showed reduced cell wall-bound peroxidase activity and decreased oxidative stress tolerance. In addition, atexpb2 exhibited lower chlorophyll contents and the germination rate compared to wild type (WT). Taken together, these results provided a new insight on the role of expansin proteins in plant stress tolerance by cell wall bound peroxidase.
authors.affiliation: State Key Laboratory of Crop Biology, Shandong Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Tai'an, Shandong 271018, PR China; Plastic Surgery Institute of Weifang Medical University, Weifang, Shandong 261041, PR China. Electronic address: hanyy2009@163.com.
authors.forename: Yangyang
authors.initials: Y
authors.lastname: Han
authors.affiliation: State Key Laboratory of Crop Biology, Shandong Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Tai'an, Shandong 271018, PR China. Electronic address: cyh890324@163.com.
authors.forename: Yanhui
authors.initials: Y
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Crop Biology, Shandong Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Tai'an, Shandong 271018, PR China. Electronic address: kaixiaoyin@163.com.
authors.forename: Suhong
authors.initials: S
authors.lastname: Yin
authors.affiliation: State Key Laboratory of Crop Biology, Shandong Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Tai'an, Shandong 271018, PR China. Electronic address: janet5207@126.com.
authors.forename: Meng
authors.initials: M
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Crop Biology, Shandong Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Tai'an, Shandong 271018, PR China. Electronic address: wangw@sdau.edu.cn.
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
mesh_terms: D000975:Antioxidants / Q000378:metabolism; D002473:Cell Wall / Q000201:enzymology; D002734:Chlorophyll / Q000378:metabolism; D015870:Gene Expression; D018525:Germination; D006540:Herbicides / Q000633:toxicity; D018384:Oxidative Stress / Q000187:drug effects; D010269:Paraquat / Q000633:toxicity; D010544:Peroxidases / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D030821:Plants, Genetically Modified; D013312:Stress, Physiological; D014026:Nicotiana / Q000187:drug effects / Q000235:genetics / Q000502:physiology*; D019076:Transgenes; D014908:Triticum / Q000235:genetics*
chemical_list: D000975:Antioxidants; D006540:Herbicides; D010940:Plant Proteins; C095825:expansin protein, plant; D002734:Chlorophyll; D010544:Peroxidases; D010269:Paraquat
keywords: Abiotic stress; Antioxidant enzyme; Cell wall peroxidase; Expansin; Methyl viologen