title: Studies on resin purification process optimization of Eucommia ulmoides Oliver and its antihypertensive effect mechanism.
abstract: BACKGROUND
  Through research it is found that the barks, stems, fruits and flowers of Eucommia ulmoides contain various compositions, such as lignin, cycloalkane and phenylpropanoids. The modern pharmacological research shows that Eucommia ulmoides has many functions, such as anti-aging, antitumor and bone cell proliferation. This paper studied the resin purification process optimization conditions and antihypertensive effect of Eucommia ulmoides Oliver.
  
  
  METHODS
  The orthogonal experiment method was adopted to determine the purification effect of various macro-porous resins for eucommia ulmoides Oliver, and the SHR model was used to determine antihypertensive level of eucommia ulmoides Oliver.
  
  
  RESULTS
  The optimal process of resin extraction and purification of Eucommia ulmoides Oliver was verified through the loading liquid concentration at 6 mg/ml, 20% ethanol used as the eluent; the flow rate control was 3.0, column volume per hour and the diameter-height ratio of adsorption column was 1:6; the extracts of Eucommia ulmoides Oliver can reduce SHR blood pressure, its antihypertensive effect is relatively stable and has no significant impact on HR, but the antihypertensive effect is lower than that of control medicine captopril. Also, the extracts of eucommia ulmoides Oliver can significantly increase the content of serum NO and reduce the content of ET.
  
  
  CONCLUSIONS
  The Eucommia ulmoides Oliver can relax blood vessels, reduce the peripheral resistance, reduce the returned blood volume, and eventually achieve the antihypertensive effect.
authors.affiliation: Department of Cardiology, the second Hospital of Jilin University, Changchun, 130041, China.
authors.forename: Liu
authors.initials: L
authors.lastname: Yongsheng
authors.affiliation: Department of Cardiology, the second Hospital of Jilin University, Changchun, 130041, China.
authors.forename: Li
authors.initials: L
authors.lastname: Shumei
authors.affiliation: Department of Cardiology, the first Hospital of Jilin University, Changchun, 130031, China.
authors.forename: Wu
authors.initials: W
authors.lastname: Guodong
mesh_terms: D000818:Animals; D000959:Antihypertensive Agents / Q000008:administration & dosage* / Q000032:analysis / Q000302:isolation & purification*; D001794:Blood Pressure / Q000187:drug effects; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage* / Q000032:analysis / Q000302:isolation & purification*; D031283:Eucommiaceae / Q000737:chemistry*; D006801:Humans; D006973:Hypertension / Q000188:drug therapy* / Q000503:physiopathology; D008297:Male; D017207:Rats, Sprague-Dawley; D012117:Resins, Synthetic / Q000737:chemistry
chemical_list: D000959:Antihypertensive Agents; D004365:Drugs, Chinese Herbal; D012117:Resins, Synthetic
keywords: AB-8 Type Macroporous Resin; Eucommia ulmoides Oliver; SHR