title: OVA12, a novel tumor antigen, promotes cancer cell growth and inhibits 5-fluorouracil-induced apoptosis.
abstract: To achieve a better understanding of mechanisms that underlie carcinogenesis and to identify novel target molecules for diagnosis and therapy of carcinoma, we previously identified 24 distinct gene clones by immunoscreening of a cDNA library derived from an ovarian cancer patient through SEREX analysis. Among these genes we focused on a novel gene termed OVA12 and which putatively encodes a 114-amino-acid protein. In the present study, we found that OVA12 was ubiquitously overexpressed in diverse human tumor cell lines. Interestingly, we noticed that overexpression of OVA12 promoted proliferation of cancer cells in vitro and accelerated tumor growth in nude mice as compared to controls. Conversely, specific downregulation of OVA12 inhibited tumor cell proliferation and tumor growth both in vitro and in vivo. Furthermore, OVA12 inhibited 5-FU-induced apoptosis through specific upregulation of Mcl-1 and survivin. These results demonstrate that OVA12 is able to promote tumor growth, suggesting that this antigen might be a new potential target for development of cancer therapy.
authors.affiliation: Shanghai Institute of Immunology, Shanghai Jiao Tong University School of Medicine, 280 South Chong-Qing Road, Shanghai 200025, China.
authors.forename: Renfeng
authors.initials: R
authors.lastname: Zhang
authors.affiliation: Department of Clinical Laboratory, People's Hospital of Shanghai Putuo District, Shanghai 200060, China.
authors.forename: Shu
authors.initials: S
authors.lastname: Jin
authors.affiliation: Shanghai Institute of Immunology, Shanghai Jiao Tong University School of Medicine, 280 South Chong-Qing Road, Shanghai 200025, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Rao
authors.affiliation: Shanghai Institute of Immunology, Shanghai Jiao Tong University School of Medicine, 280 South Chong-Qing Road, Shanghai 200025, China.
authors.forename: Feifei
authors.initials: F
authors.lastname: Song
authors.affiliation: Shanghai Institute of Immunology, Shanghai Jiao Tong University School of Medicine, 280 South Chong-Qing Road, Shanghai 200025, China.
authors.forename: Qinqin
authors.initials: Q
authors.lastname: Yin
authors.affiliation: Shanghai Institute of Immunology, Shanghai Jiao Tong University School of Medicine, 280 South Chong-Qing Road, Shanghai 200025, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Shanghai Institute of Immunology, Shanghai Jiao Tong University School of Medicine, 280 South Chong-Qing Road, Shanghai 200025, China.
authors.forename: Li
authors.initials: L
authors.lastname: Wang
authors.affiliation: Shanghai Institute of Immunology, Shanghai Jiao Tong University School of Medicine, 280 South Chong-Qing Road, Shanghai 200025, China.
authors.forename: Yebin
authors.initials: Y
authors.lastname: Xi
authors.affiliation: Key Laboratory of Stem Cell Biology, Institute of Health Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences and Shanghai JiaoTong University School of Medicine, Shanghai 200031, China.
authors.forename: Xiaoren
authors.initials: X
authors.lastname: Zhang
authors.affiliation: General Surgery Department, Rui Jin Hospital Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China. Electronic address: mingliang-99@hotmail.com.
authors.forename: Mingliang
authors.initials: M
authors.lastname: Wang
authors.affiliation: Shanghai Institute of Immunology, Shanghai Jiao Tong University School of Medicine, 280 South Chong-Qing Road, Shanghai 200025, China. Electronic address: gehl@shsmu.edu.cn.
authors.forename: Hailiang
authors.initials: H
authors.lastname: Ge
mesh_terms: D000818:Animals; D000951:Antigens, Neoplasm / Q000235:genetics / Q000378:metabolism*; D017209:Apoptosis / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D005260:Female; D005472:Fluorouracil / Q000037:antagonists & inhibitors* / Q000494:pharmacology; D006367:HeLa Cells; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009369:Neoplasms / Q000188:drug therapy* / Q000378:metabolism* / Q000473:pathology; D014162:Transfection; D023041:Xenograft Model Antitumor Assays
chemical_list: D000951:Antigens, Neoplasm; D005472:Fluorouracil
keywords: 5-FU; Mcl-1; OVA12; Survivin; Tumor antigen; Tumorigenesis