title: Precision of dehydroascorbic acid quantitation with the use of the subtraction method--validation of HPLC-DAD method for determination of total vitamin C in food.
abstract: In food analysis, a method for determination of vitamin C should enable measuring of total content of ascorbic acid (AA) and dehydroascorbic acid (DHAA) because both chemical forms exhibit biological activity. The aim of the work was to confirm applicability of HPLC-DAD method for analysis of total content of vitamin C (TC) and ascorbic acid in various types of food by determination of validation parameters such as: selectivity, precision, accuracy, linearity and limits of detection and quantitation. The results showed that the method applied for determination of TC and AA was selective, linear and precise. Precision of DHAA determination by the subtraction method was also evaluated. It was revealed that the results of DHAA determination obtained by the subtraction method were not precise which resulted directly from the assumption of this method and the principles of uncertainty propagation. The proposed chromatographic method should be recommended for routine determinations of total vitamin C in various food.
authors.affiliation: Department of Analysis and Evaluation of Food Quality, University of Life Sciences, Skromna 8, 20-704 Lublin, Poland. Electronic address: artur.mazurek@up.lublin.pl.
authors.forename: Artur
authors.initials: A
authors.lastname: Mazurek
authors.affiliation: Department of Analysis and Evaluation of Food Quality, University of Life Sciences, Skromna 8, 20-704 Lublin, Poland.
authors.forename: Jerzy
authors.initials: J
authors.lastname: Jamroz
mesh_terms: D001205:Ascorbic Acid / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003683:Dehydroascorbic Acid / Q000032:analysis*; D005504:Food Analysis / Q000379:methods*; D057230:Limit of Detection
chemical_list: D001205:Ascorbic Acid; D003683:Dehydroascorbic Acid
keywords: Ascorbic acid; Ascorbic acid (PubChem CID: 54670067); Dehydroascorbic acid; Dehydroascorbic acid (PubChem CID: 10280); Subtraction method; Validation; Vitamin C