title: Hormone replacement with 17β-estradiol plus dihydrotestosterone restores male sexual behavior in rats treated neonatally with clomipramine.
abstract: Male sexual behavior (MSB) in rodents, in both its consummatory and motivational components, is regulated by hormones such as testosterone, 17β-estradiol and 5-α-dihydrotestosterone. In experiments, neonatal treatment with clomipramine (CMI; a serotonin reuptake inhibitor) reproduces some of the signs of depression in adult age, including reduced sexual behavior manifested in a lower percentage of subjects that mount, intromit and ejaculate, although their testosterone levels were not altered. However, the effect of this treatment on estrogen levels and the consequences of hormone substitution using 17β-estradiol and 5-α-dihydrotestosterone on the expression of male sexual behavior are still unknown. Therefore, the objective of the present study was to analyze the effect of neonatal treatment with CMI on plasma testosterone and 17β-estradiol levels, and the role of testosterone, 17β-estradiol and 5-α-dihydrotestosterone in altering the consummatory and motivational components of sexual behavior in male rats. To this end, it analyzed the copulatory parameters and sexual incentive motivation (SIM) of rats treated with CMI under two conditions: basal and post-hormone replacements. Neonatal treatment with CMI did not affect plasma testosterone or 17β-estradiol concentrations, but did decrease both the consummatory component and sexual motivation according to the results of the SIM test. These aspects were recovered after administering 17β-estradiol +5-α-dihydrotestosterone, but not testosterone.
authors.affiliation: Posgrado en Biología Experimental, Universidad Autónoma Metropolitana-Iztapalapa. Apartado, Postal 55 535, C.P. 09340, D.F. México.
authors.forename: Ofelia
authors.initials: O
authors.lastname: Limón-Morales
authors.affiliation: Laboratorio de Psicobiologia, Centro Universitario de los Lagos, Universidad de Guadalajara, México.
authors.forename: Cesar
authors.initials: C
authors.lastname: Soria-Fregozo
authors.affiliation: Departamento de Biología de la Reproducción, Universidad Autónoma Metropolitana-Iztapalapa. Apartado Postal 55 535, C.P. 09340, D.F. México.
authors.forename: Marcela
authors.initials: M
authors.lastname: Arteaga-Silva
authors.affiliation: Instituto de Neurociencias, Universidad de Guadalajara, Francisco de Quevedo 180, Col. Arcos de Vallarta, CP 44130, Guadalajara, Jalisco, México.
authors.forename: Marisela Hernández
authors.initials: MH
authors.lastname: González
authors.affiliation: Colegio De Ciencia y Tecnología, Universidad Autónoma de la Ciudad de México-San Lorenzo Tezonco, Av. Prolongación San Isidro 151, Col. San Lorenzo Tezonco, Deleg, Iztapalapa CP 09790, México.
authors.forename: Gonzalo
authors.initials: G
authors.lastname: Vázquez-Palacios
authors.affiliation: Departamento de Biología de la Reproducción, Universidad Autónoma Metropolitana-Iztapalapa. Apartado Postal 55 535, C.P. 09340, D.F. México. Electronic address: bjh@xanum.uam.mx.
authors.forename: Herlinda
authors.initials: H
authors.lastname: Bonilla-Jaime
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D002997:Clomipramine / Q000009:adverse effects*; D013196:Dihydrotestosterone / Q000008:administration & dosage / Q000494:pharmacology*; D004958:Estradiol / Q000008:administration & dosage / Q000494:pharmacology*; D008297:Male; D009042:Motivation / Q000187:drug effects; D009919:Orchiectomy; D051381:Rats; D017208:Rats, Wistar; D012725:Sexual Behavior / Q000187:drug effects*; D012726:Sexual Behavior, Animal / Q000187:drug effects*; D012741:Sexual Maturation / Q000187:drug effects; D013739:Testosterone / Q000097:blood
chemical_list: D013196:Dihydrotestosterone; D013739:Testosterone; D004958:Estradiol; D002997:Clomipramine
keywords: 17β-estradiol; Clomipramine (CMI); Depression; Gonadal hormones; Motivation; Sexual behavior