title: Expression of variant transcripts of the potassium channel tetramerization domain-containing 15 (KCTD15) gene and their association with fatness traits in chickens.
abstract: The aim of this study was to characterize the structure, expression, and biological functions of potassium channel tetramerization domain containing 15 (KCTD15) in chickens. We compared the KCTD15 expression level in samples of hypothalamic, adipose, and liver tissue of Xinghua chickens that were maintained on different dietary status. An association analysis of KCTD15 gene variant transcripts with fatness traits in a F2 resource population of chickens was performed. Three KCTD15 transcripts were identified in which the complete transcript was predominantly expressed in adipose tissue and the hypothalamus. The chicken KCTD15 gene was regulated by both feeding and fasting and consumption of a high-fat diet. The expression level of KCTD15 gene was markedly decreased in hypothalamus and liver of fasted and refed chickens (P < 0.05) and significantly downregulated in adipose tissue by the high-fat diet (P < 0.05). Three single-nucleotide polymorphisms of the KCTD15 gene were significantly associated with a number of fatness traits in chicken (P < 0.05). These results suggest that KCTD15 have a potential role regulation of obesity and fat metabolism in chickens.
authors.affiliation: Department of Animal Genetics, Breeding and Reproduction, College of Animal Science, South China Agricultural University, Guangzhou 510642, Guangdong, China; Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding and Key Laboratory of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture, Guangzhou 510642, Guangdong, China.
authors.forename: S S
authors.initials: SS
authors.lastname: Liang
authors.affiliation: Department of Animal Genetics, Breeding and Reproduction, College of Animal Science, South China Agricultural University, Guangzhou 510642, Guangdong, China; Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding and Key Laboratory of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture, Guangzhou 510642, Guangdong, China.
authors.forename: H J
authors.initials: HJ
authors.lastname: Ouyang
authors.affiliation: Department of Animal Genetics, Breeding and Reproduction, College of Animal Science, South China Agricultural University, Guangzhou 510642, Guangdong, China; Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding and Key Laboratory of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture, Guangzhou 510642, Guangdong, China.
authors.forename: J
authors.initials: J
authors.lastname: Liu
authors.affiliation: Department of Animal Genetics, Breeding and Reproduction, College of Animal Science, South China Agricultural University, Guangzhou 510642, Guangdong, China; Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding and Key Laboratory of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture, Guangzhou 510642, Guangdong, China.
authors.forename: B
authors.initials: B
authors.lastname: Chen
authors.affiliation: Department of Animal Genetics, Breeding and Reproduction, College of Animal Science, South China Agricultural University, Guangzhou 510642, Guangdong, China; Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding and Key Laboratory of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture, Guangzhou 510642, Guangdong, China. Electronic address: nqinghua@scau.edu.cn.
authors.forename: Q H
authors.initials: QH
authors.lastname: Nie
authors.affiliation: Department of Animal Genetics, Breeding and Reproduction, College of Animal Science, South China Agricultural University, Guangzhou 510642, Guangdong, China; Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding and Key Laboratory of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture, Guangzhou 510642, Guangdong, China.
authors.forename: X Q
authors.initials: XQ
authors.lastname: Zhang
mesh_terms: D050154:Adiposity / Q000235:genetics* / Q000502:physiology; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002645:Chickens / Q000235:genetics* / Q000502:physiology*; D005260:Female; D005786:Gene Expression Regulation / Q000502:physiology; D014644:Genetic Variation; D005838:Genotype; D008297:Male; D008969:Molecular Sequence Data; D010802:Phylogeny; D020641:Polymorphism, Single Nucleotide; D015221:Potassium Channels / Q000235:genetics / Q000378:metabolism*; D016415:Sequence Alignment
chemical_list: D015221:Potassium Channels
keywords: Association analysis; Chicken; Fatness; Gene expression; KCTD15