title: Controlled hydrostatic pressure stress downregulates the expression of ribosomal genes in preimplantation embryos: a possible protection mechanism?
abstract: The efficiency of various assisted reproductive techniques can be improved by preconditioning the gametes and embryos with sublethal hydrostatic pressure treatment. However, the underlying molecular mechanism responsible for this protective effect remains unknown and requires further investigation. Here, we studied the effect of optimised hydrostatic pressure treatment on the global gene expression of mouse oocytes after embryonic genome activation. Based on a gene expression microarray analysis, a significant effect of treatment was observed in 4-cell embryos derived from treated oocytes, revealing a transcriptional footprint of hydrostatic pressure-affected genes. Functional analysis identified numerous genes involved in protein synthesis that were downregulated in 4-cell embryos in response to hydrostatic pressure treatment, suggesting that regulation of translation has a major role in optimised hydrostatic pressure-induced stress tolerance. We present a comprehensive microarray analysis and further delineate a potential mechanism responsible for the protective effect of hydrostatic pressure treatment.
authors.affiliation: Molecular Animal Biotechnology Laboratory, Szent Istvan University, Pater Karoly u. 1. 2100 Godollo, Hungary.
authors.forename: I
authors.initials: I
authors.lastname: Bock
authors.affiliation: Biotalentum Ltd., Aulich Lajos u. 26. 2100 Godollo, Hungary.
authors.forename: H
authors.initials: H
authors.lastname: Raveh-Amit
authors.affiliation: Department of Animal Breeding and Genetics, Faculty of Veterinary Science, Szent Istvan University, Istvan u. 2. 1078 Budapest, Hungary.
authors.forename: E
authors.initials: E
authors.lastname: Losonczi
authors.affiliation: Biotalentum Ltd., Aulich Lajos u. 26. 2100 Godollo, Hungary.
authors.forename: A C
authors.initials: AC
authors.lastname: Carstea
authors.affiliation: Biotalentum Ltd., Aulich Lajos u. 26. 2100 Godollo, Hungary.
authors.forename: A
authors.initials: A
authors.lastname: Feher
authors.affiliation: Biotalentum Ltd., Aulich Lajos u. 26. 2100 Godollo, Hungary.
authors.forename: K
authors.initials: K
authors.lastname: Mashayekhi
authors.affiliation: Laboratory of Embryology, Kaali Institute, Istenhegyi ut 54/A. 1125 Budapest, Hungary.
authors.forename: S
authors.initials: S
authors.lastname: Matyas
authors.affiliation: Molecular Animal Biotechnology Laboratory, Szent Istvan University, Pater Karoly u. 1. 2100 Godollo, Hungary.
authors.forename: A
authors.initials: A
authors.lastname: Dinnyes
authors.affiliation: Department of Animal Breeding and Genetics, Faculty of Veterinary Science, Szent Istvan University, Istvan u. 2. 1078 Budapest, Hungary.
authors.forename: C
authors.initials: C
authors.lastname: Pribenszky
mesh_terms: D000818:Animals; D021461:Animals, Outbred Strains; D001755:Blastocyst / Q000166:cytology / Q000201:enzymology / Q000378:metabolism*; D015536:Down-Regulation; D004477:Ectogenesis; D046149:Embryo Culture Techniques; D004622:Embryo, Mammalian / Q000166:cytology / Q000201:enzymology / Q000378:metabolism*; D005260:Female; D020869:Gene Expression Profiling; D006874:Hydrostatic Pressure / Q000009:adverse effects; D008297:Male; D051379:Mice; D020411:Oligonucleotide Array Sequence Analysis; D009865:Oocytes / Q000166:cytology / Q000201:enzymology / Q000378:metabolism*; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000378:metabolism; D012269:Ribosomal Proteins / Q000235:genetics / Q000378:metabolism*; D013047:Specific Pathogen-Free Organisms; D020554:Sperm Injections, Intracytoplasmic; D013312:Stress, Physiological
chemical_list: D012333:RNA, Messenger; D012269:Ribosomal Proteins