title: Temporal evolution and potential recombination events in PRRSV strains of Sonora Mexico.
abstract: The aim of this work was to examine the evolution and potential existence of intragenic recombinations of PRRSV strains in Sonora, Mexico. In this study, 142 serum samples from farms located in Hermosillo (HMO), Cd. Obregón (OBR) and Navojoa (NAV) were sequenced from 2002 to 2012. Ninety non-redundant sequences of ORF5 gene were analyzed for temporal and spatial relationships among strains and the probability of a recombination event. The phylogenetic analysis showed 30 strains grouped into eight groups; 16 strains were closely related among the farms, while 14 were un-related. The first strain in this study was observed in 2002. A number of farms were infected with one or more strains, and in the majority of the strains, the virus was replaced by a new strain. The recombination analysis suggested the presence of four viruses as products of a recombination event; in one case, a virus close related with MLV vaccine was involved as the parent virus. This work shows the evolution of PRRSV in the field, the viral dissemination between farms and the potential recombination events. Our data suggest that PRRSV in Sonora has a specific genetic nature compared with other PRRSV.
authors.affiliation: Laboratorio de Inmunología, Centro de Investigación en Alimentación y Desarrollo A.C., Hermosillo, Sonora, Mexico.
authors.forename: Alexel
authors.initials: A
authors.lastname: Burgara-Estrella
authors.affiliation: Laboratorio de Inmunología, Centro de Investigación en Alimentación y Desarrollo A.C., Hermosillo, Sonora, Mexico.
authors.forename: Mónica
authors.initials: M
authors.lastname: Reséndiz-Sandoval
authors.affiliation: National Veterinary Institute, Ullsväg 2B, SE-751 89 Uppsala, Sweden.
authors.forename: Martí
authors.initials: M
authors.lastname: Cortey
authors.affiliation: Centre de Recerca en Sanitat Animal, UAB-IRTA, Campus de la Universitat Autònoma de Barcelona, Bellaterra, Barcelona, Spain; Departament de Sanitat i Anatomia Animals, Universitat Autònoma de Barcelona, Bellaterra, Barcelona, Spain.
authors.forename: Enric
authors.initials: E
authors.lastname: Mateu
authors.affiliation: Laboratorio de Inmunología, Centro de Investigación en Alimentación y Desarrollo A.C., Hermosillo, Sonora, Mexico. Electronic address: jhdez@ciad.mx.
authors.forename: Jesús
authors.initials: J
authors.lastname: Hernández
mesh_terms: D000818:Animals; D001483:Base Sequence; D016000:Cluster Analysis; D019143:Evolution, Molecular; D014644:Genetic Variation; D008800:Mexico / Q000453:epidemiology; D008969:Molecular Sequence Data; D010802:Phylogeny; D019318:Porcine Reproductive and Respiratory Syndrome / Q000097:blood / Q000453:epidemiology / Q000821:virology*; D019316:Porcine respiratory and reproductive syndrome virus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D011995:Recombination, Genetic; D017422:Sequence Analysis, DNA / Q000662:veterinary; D013552:Swine; D014759:Viral Envelope Proteins / Q000235:genetics
chemical_list: D014759:Viral Envelope Proteins; C114789:glycoprotein 5, PRRSV
keywords: Genetic diversity; ORF5; PRRSV; Recombination