title: In vivo effect of deoxynivalenol (DON) naturally contaminated feed on porcine reproductive and respiratory syndrome virus (PRRSV) infection.
abstract: Deoxynivalenol (DON), also known as vomitoxin, is the most prevalent type B trichothecene mycotoxin worldwide. Pigs show a great sensitivity to DON, and because of the high proportion of grains in their diets, they are frequently exposed to this mycotoxin. The objective of this study was to determine the impact of DON naturally contaminated feed on porcine reproductive and respiratory syndrome virus (PRRSV) infection, the most important porcine viral pathogen in swine. Experimental infections were performed with 30 animals. Piglets were randomly divided into three groups of 10 animals based on DON content of diets (0, 2.5 and 3.5 mg/kg DON). All experimental groups were further divided into subgroups of 6 pigs and were inoculated with PRRSV. The remaining pigs (control) were sham-inoculated with PBS. Pigs were daily monitored for temperature, weight and clinical signs for 21 days. Blood samples were collected and tested for PRRSV RNA and for virus specific antibodies. Results of PRRSV infection showed that ingestion of diet highly contaminated with DON greatly increases the effect of PRRSV infection on weight gain, lung lesions and mortality, without increasing significantly viral replication, for which the tendency is rather directed toward a decrease of replication. These results suggest that PRRSV infection could exacerbate anorectic effect of DON, when ingested in large doses. Results also demonstrate a DON negative effect on PRRSV-specific humoral responses. This study demonstrate that high concentrations of DON naturally contaminated feed decreased the immune response against PRRSV and influenced the course of PRRSV infection in pigs.
authors.affiliation: Groupe de Recherche sur les Maladies Infectieuses du Porc (GREMIP), Swine and Poultry Infectious Diseases Research Center (CRIPA), Saint-Hyacinthe, Québec, Canada.
authors.forename: Christian
authors.initials: C
authors.lastname: Savard
authors.affiliation: Département de Biomédecine Vétérinaire, Saint-Hyacinthe, Québec, Canada.
authors.forename: Vicente
authors.initials: V
authors.lastname: Pinilla
authors.affiliation: Groupe de Recherche sur les Maladies Infectieuses du Porc (GREMIP), Swine and Poultry Infectious Diseases Research Center (CRIPA), Saint-Hyacinthe, Québec, Canada.
authors.forename: Chantale
authors.initials: C
authors.lastname: Provost
authors.affiliation: Groupe de Recherche sur les Maladies Infectieuses du Porc (GREMIP), Swine and Poultry Infectious Diseases Research Center (CRIPA), Saint-Hyacinthe, Québec, Canada.
authors.forename: Carl A
authors.initials: CA
authors.lastname: Gagnon
authors.affiliation: Département de Biomédecine Vétérinaire, Saint-Hyacinthe, Québec, Canada. Electronic address: Younes.chorfi@umontreal.ca.
authors.forename: Younes
authors.initials: Y
authors.lastname: Chorfi
mesh_terms: D000821:Animal Feed; D000818:Animals; D000914:Antibodies, Viral / Q000187:drug effects*; D018928:Immunity, Mucosal / Q000187:drug effects; D009183:Mycotoxins / Q000494:pharmacology*; D019318:Porcine Reproductive and Respiratory Syndrome / Q000276:immunology* / Q000821:virology; D019316:Porcine respiratory and reproductive syndrome virus / Q000187:drug effects* / Q000276:immunology / Q000502:physiology; D013552:Swine; D013553:Swine Diseases / Q000276:immunology* / Q000821:virology; D014255:Trichothecenes / Q000494:pharmacology*; D014779:Virus Replication
chemical_list: D000914:Antibodies, Viral; D009183:Mycotoxins; D014255:Trichothecenes; C007262:deoxynivalenol
keywords: DON mycotoxin; PRRSV; Pig; Predisposition to infection