title: Simultaneous determination of total nitrogen and total phosphorus in environmental waters using alkaline persulfate digestion and ion chromatography.
abstract: An ion chromatography (IC) method was developed for the simultaneous determination of total nitrogen and total phosphorus after alkaline persulfate digestion. This study takes advantage of advances in construction of high-resolution, high-capacity anion-exchange columns that can better tolerate the matrices typically encountered when a determination of total nitrogen and total phosphorous is required. Here, we used an electrolytically generated hydroxide eluent combined with a high-capacity, hydroxide-selective, anion-exchange column for the determination of total nitrogen (as nitrate-N) and total phosphorus (as phosphate-P) in environmental samples by IC. This method yielded LODs for nitrate-N and phosphate-P of 1.0 and 1.3 μg/L, respectively. The LOQs determined for these analytes were 3.4 and 4.2 μg/L, respectively. Due to the dilution factor required and the blank nitrate-N concentration after the persulfate digestion, the quantification limits increased for nitrate-N and phosphate-P to 171 and 63 μg/L, respectively. The suitability of the method was evaluated by determining the nitrogen and phosphorus concentrations from known concentrations of organic-containing nitrogen and phosphorus compounds. In addition, environmental samples consisting of six different wastewaters and 48 reservoir samples were evaluated for total nitrogen and phosphorus. The recoveries of nitrogen and phosphorus from the organic-containing compounds ranged from 93.1 to 100.1% and 85.2 to 97.1%, respectively. In addition, good correlation between results obtained by the colorimetric method and IC was also observed. The linearity, accuracy, and evaluation of potential interferences for determining TN and TP will be discussed.
authors.affiliation: Thermo Fisher Scientific, 1214 Oakmead Parkway, Sunnyvale, CA 94085-4024, USA. Electronic address: brian.deborba@thermofisher.com.
authors.forename: Brian M
authors.initials: BM
authors.lastname: De Borba
authors.affiliation: Thermo Fisher Scientific, 1214 Oakmead Parkway, Sunnyvale, CA 94085-4024, USA.
authors.forename: Richard F
authors.initials: RF
authors.lastname: Jack
authors.affiliation: Thermo Fisher Scientific, 1214 Oakmead Parkway, Sunnyvale, CA 94085-4024, USA.
authors.forename: Jeffrey S
authors.initials: JS
authors.lastname: Rohrer
authors.affiliation: Occoquan Laboratory, 9408 Prince William Street, Manassas, VA 20110-5666, USA.
authors.forename: Joan
authors.initials: J
authors.lastname: Wirt
authors.affiliation: Occoquan Laboratory, 9408 Prince William Street, Manassas, VA 20110-5666, USA.
authors.forename: Dongmei
authors.initials: D
authors.lastname: Wang
mesh_terms: D000468:Alkalies / Q000737:chemistry; D002852:Chromatography, Ion Exchange / Q000295:instrumentation / Q000379:methods*; D057230:Limit of Detection; D009566:Nitrates / Q000032:analysis*; D009584:Nitrogen / Q000032:analysis*; D010758:Phosphorus / Q000032:analysis*; D013440:Sulfides / Q000737:chemistry*; D014867:Water / Q000737:chemistry*
chemical_list: D000468:Alkalies; D009566:Nitrates; D013440:Sulfides; C051552:persulfides; D014867:Water; D010758:Phosphorus; D009584:Nitrogen
keywords: Alkaline persulfate; Eutrophication; Ion chromatography; Nutrient pollution; Total nitrogen; Total phosphorus