title: Valosin-containing protein (VCP/p97)-expression correlates with prognosis of HPV- negative oropharyngeal squamous cell carcinoma (OSCC).
abstract: Valosin-containing protein (VCP)/p97 has been shown to be associated with antiapoptotic function via activation of the nuclear factor-[Formula: see text]B (NF[Formula: see text]B) signaling pathway and with metastasizing of tumors in several studies. VCP is located on chromosome 9p13-p12, a region often deleted in oropharyngeal squamous cell carcinoma (OSCC). The clinical significance of VCP expression in OSCC however remains unclear. In this study, expression of VCP was determined in 106 patients (77 male (71.3%) and 31 female (28.7%); age-range: 34-79 years (mean age 57 years)) by immunohistochemistry and in a subset of 15 patients by quantitative PCR. HPV-DNA was detected by polymerase chain reaction and p16INK4a immunohistochemistry. The experimental findings were correlated with clinico-pathological data and survival parameters. 47.2% of all OSCC specimens were analyzed as negative or weak staining intensity for VCP. 52.8% of all specimens showed a high staining intensity for VCP. 73.1% of all patients were tested HPV-negative, 26.9% were HPV-positive. The 5-year disease-free and overall survival probabilities of all patients were 71.2% and 55.7%, respectively. No correlation could be found between HPV-status and VCP expression. VCP overexpression in HPV-negative patients was associated with significantly better 5-year disease-free survival (86.4% vs., 45.6%, p = 0.017). The level of VCP-intensity determined by immunohistochemistry could be an additional prognostic marker in HPV-negative OSCC. VCP expression seems not to correlate with the HPV-status.
authors.affiliation: Department of Otorhinolaryngology, Head and Neck Surgery, University of Cologne, Cologne, Germany.
authors.forename: Moritz F
authors.initials: MF
authors.lastname: Meyer
authors.affiliation: Department of Otorhinolaryngology, Head and Neck Surgery, University of Cologne, Cologne, Germany.
authors.forename: Inga M C
authors.initials: IM
authors.lastname: Seuthe
authors.affiliation: Department of Pathology, University of Cologne, Cologne, Germany.
authors.forename: Uta
authors.initials: U
authors.lastname: Drebber
authors.affiliation: Jean-Uhmacher Institute, University of Cologne, Cologne, Germany.
authors.forename: Oliver
authors.initials: O
authors.lastname: Siefer
authors.affiliation: Department for Oral and Maxillofacial Plastic Surgery, University of Cologne, Cologne, Germany.
authors.forename: Matthias
authors.initials: M
authors.lastname: Kreppel
authors.affiliation: Clinic for Oral and Maxillofacial Plastic Surgery, Dusseldorf, Germany.
authors.forename: Marcus O
authors.initials: MO
authors.lastname: Klein
authors.affiliation: Department of Otorhinolaryngology, Head and Neck Surgery, University of Cologne, Cologne, Germany.
authors.forename: Stefanie
authors.initials: S
authors.lastname: Mikolajczak
authors.affiliation: Department of Otorhinolaryngology, Head and Neck Surgery, University of Giessen, Giessen, Germany.
authors.forename: Jens Peter
authors.initials: JP
authors.lastname: Klussmann
authors.affiliation: Department of Otorhinolaryngology, Head and Neck Surgery, University of Cologne, Cologne, Germany.
authors.forename: Simon F
authors.initials: SF
authors.lastname: Preuss
authors.affiliation: Jean-Uhmacher Institute, University of Cologne, Cologne, Germany.
authors.forename: Christian U
authors.initials: CU
authors.lastname: Huebbers
mesh_terms: D000251:Adenosine Triphosphatases / Q000235:genetics / Q000378:metabolism*; D000328:Adult; D000368:Aged; D002294:Carcinoma, Squamous Cell / Q000175:diagnosis* / Q000378:metabolism; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism*; D018572:Disease-Free Survival; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009959:Oropharyngeal Neoplasms / Q000175:diagnosis* / Q000378:metabolism; D030361:Papillomavirus Infections / Q000150:complications; D011379:Prognosis; D016019:Survival Analysis; D000074405:Valosin Containing Protein
chemical_list: D018797:Cell Cycle Proteins; D000251:Adenosine Triphosphatases; C000614178:VCP protein, human; D000074405:Valosin Containing Protein