title: The fabrication of superlow protein absorption zwitterionic coating by electrochemically mediated atom transfer radical polymerization and its application.
abstract: A well-controllable electrochemically mediated surface-initiated atom transfer radical polymerization (e-siATRP) method for the fabrication of superlow protein absorption zwitterionic hydrogel coatings based on poly(sulbetaine methacrylate) (pSBMA) was developed in this work. The effects of the electric condition on polymerization as well as its antifouling performances both in vitro and in vivo were also investigated. Different potentials (-0.08 V, -0.15 V and -0.22 V) and polymerization times (from 8 to 48 h) were chosen to study the polymerization procedure. X-ray photoelectron spectroscopy, atomic force microscopy and ellipsometry measurements were used to characterize the properties of the polymer layers. Ellipsometry measurements showed that a higher potential provided faster polymerization and thicker polymer layers; however, the protein absorption experiments showed that the best polymerization condition was under a constant potential of -0.15 V and 32 h, under which the protein absorption was 0.8% in an enzyme-linked immunosorbent assay (compared to a bare gold electrode). The electrodes with a pSBMA coating effectively deduced the current sensitivity decay both in undiluted serum and in vivo. The usage of the commercially available polymerization monomer of SBMA, the simple convenient synthesis process regardless of the presence of oxygen and the excellent controllability of e-siATRP make it a very promising and universal technique in the preparation of zwitterionic polymer coatings, especially in the development of biocompatible material for implantable devices such as neural and biosensor electrodes.
authors.affiliation: Biosensor National Special Laboratory, Key Laboratory of Biomedical Engineering of Ministry of Education, College of Biomedical Engineering & Instrument Science, Hangzhou 310027, People's Republic of China; Zhijiang College, Zhejiang University of Technology, Hangzhou 310027, People's Republic of China.
authors.forename: Yichuan
authors.initials: Y
authors.lastname: Hu
authors.affiliation: Biosensor National Special Laboratory, Key Laboratory of Biomedical Engineering of Ministry of Education, College of Biomedical Engineering & Instrument Science, Hangzhou 310027, People's Republic of China.
authors.forename: Guang
authors.initials: G
authors.lastname: Yang
authors.affiliation: Biosensor National Special Laboratory, Key Laboratory of Biomedical Engineering of Ministry of Education, College of Biomedical Engineering & Instrument Science, Hangzhou 310027, People's Republic of China.
authors.forename: Bo
authors.initials: B
authors.lastname: Liang
authors.affiliation: Biosensor National Special Laboratory, Key Laboratory of Biomedical Engineering of Ministry of Education, College of Biomedical Engineering & Instrument Science, Hangzhou 310027, People's Republic of China.
authors.forename: Lu
authors.initials: L
authors.lastname: Fang
authors.affiliation: State Key Laboratory of Chemical Engineering, Department of Chemical and Biological Engineering, Zhejiang University, Hangzhou 310027, People's Republic of China.
authors.forename: Guanglong
authors.initials: G
authors.lastname: Ma
authors.affiliation: Biosensor National Special Laboratory, Key Laboratory of Biomedical Engineering of Ministry of Education, College of Biomedical Engineering & Instrument Science, Hangzhou 310027, People's Republic of China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Zhu
authors.affiliation: State Key Laboratory of Chemical Engineering, Department of Chemical and Biological Engineering, Zhejiang University, Hangzhou 310027, People's Republic of China.
authors.forename: Shengfu
authors.initials: S
authors.lastname: Chen
authors.affiliation: Biosensor National Special Laboratory, Key Laboratory of Biomedical Engineering of Ministry of Education, College of Biomedical Engineering & Instrument Science, Hangzhou 310027, People's Republic of China. Electronic address: yexuesong@zju.edu.cn.
authors.forename: Xuesong
authors.initials: X
authors.lastname: Ye
mesh_terms: D000327:Adsorption; D000818:Animals; D002417:Cattle; D020099:Coated Materials, Biocompatible / Q000138:chemical synthesis / Q000737:chemistry; D004567:Electrodes, Implanted; D020100:Hydrogels / Q000138:chemical synthesis / Q000737:chemistry; D008297:Male; D008689:Methacrylates / Q000138:chemical synthesis / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley; D012710:Serum Albumin, Bovine / Q000737:chemistry*
chemical_list: D020099:Coated Materials, Biocompatible; D020100:Hydrogels; D008689:Methacrylates; C521589:sulfobetaine methacrylate polymer; D012710:Serum Albumin, Bovine
keywords: Biocompatibility; Electrochemically mediated ATRP; Nonfouling; Zwitterionic hydrogel