title: Simultaneous determination of notoginsenoside R₁, ginsenoside Rg₁, ginsenoside Re and 20(S) protopanaxatriol in beagle dog plasma by ultra high performance liquid mass spectrometry after oral administration of a Panax notoginseng saponin preparation.
abstract: 20(S) protopanaxatriol is the main metabolite of notoginsenoside R1, ginsenoside Rg1, ginsenoside Re in Panax notoginseng and has significant activities. A ultra high performance liquid mass spectrometry method has been developed and validated for the simultaneous determination of notoginsenoside R₁ (R1), ginsenoside Rg₁ (Rg₁), ginsenoside Re (Re) and 20(S) protopanaxatriol (PPT) in beagle dog plasma after oral administration of a Panax notoginseng saponin preparation. After the addition of the internal standard (digoxin), plasma samples were subjected to liquid-liquid extraction with acetone and methanol and separated on a 100 × 2.1 mm ACQUITY 1.7 μm C₁₈ column (Waters, USA), with acetonitrile and water as the mobile phase, within a runtime of 7.0 min. The analytes were detected without interference in Selected Reaction Monitoring mode with a change in the electrospray ionization from positive to negative. The detection limits were 0.01 to 0.04 mg/L and the calibration curves of the peak areas for the four ingredients were linear over four orders of magnitude with a correlation coefficient greater than 0.9957. The intra-day and inter-day precision values (relative standard deviation, RSD, %) were within 10.25% and 13.51%, respectively, and the accuracy (relative error, RE, %) was less than 7.81%. The validated method was successfully applied to a comparative pharmacokinetic study of four saponins in beagle dogs after oral administration of a Panax Notoginseng Saponins preparation. The pharmacokinetic parameters were calculated with DAS 3.20. The Tmax and Cmax values indicate a dose-dose relationship between the saponins (R1, Rg1, and Re) and their sapogenin (PPT).
authors.affiliation: School of Chinese Materia Medica, Beijing University of Chinese Medicine, No. 6, Zhonghuan South Road, Wangjing, Chaoyang District, Beijing 100102, China.
authors.forename: Huichao
authors.initials: H
authors.lastname: Wu
authors.affiliation: School of Chinese Materia Medica, Beijing University of Chinese Medicine, No. 6, Zhonghuan South Road, Wangjing, Chaoyang District, Beijing 100102, China.
authors.forename: Huimin
authors.initials: H
authors.lastname: Liu
authors.affiliation: School of Chinese Materia Medica, Beijing University of Chinese Medicine, No. 6, Zhonghuan South Road, Wangjing, Chaoyang District, Beijing 100102, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Bai
authors.affiliation: School of Chinese Materia Medica, Beijing University of Chinese Medicine, No. 6, Zhonghuan South Road, Wangjing, Chaoyang District, Beijing 100102, China. Electronic address: landocean28@163.com.
authors.forename: Yang
authors.initials: Y
authors.lastname: Lu
authors.affiliation: School of Chinese Materia Medica, Beijing University of Chinese Medicine, No. 6, Zhonghuan South Road, Wangjing, Chaoyang District, Beijing 100102, China. Electronic address: dushouying@263.net.
authors.forename: Shouying
authors.initials: S
authors.lastname: Du
mesh_terms: D000284:Administration, Oral; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004285:Dogs; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000032:analysis*; D036145:Ginsenosides / Q000008:administration & dosage / Q000097:blood*; D013058:Mass Spectrometry / Q000379:methods*; D053723:Panax notoginseng / Q000737:chemistry*; D012502:Sapogenins / Q000008:administration & dosage / Q000097:blood*; D012503:Saponins / Q000008:administration & dosage / Q000097:blood
chemical_list: D004365:Drugs, Chinese Herbal; D036145:Ginsenosides; D012502:Sapogenins; D012503:Saponins; C081552:protopanaxatriol; C049864:ginsenoside Re; C035054:ginsenoside Rg1; C072936:notoginsenoside R1
keywords: 20(S) protopanaxatriol (PPT); Ginsenoside Re; Ginsenoside Rg(1); Notoginsenoside R(1); Pharmacokinetics; Ultra high performance liquid mass spectrometry