title: Photocontrolled solid-phase extraction of guanine from complex samples using a novel photoresponsive molecularly imprinted polymer.
abstract: A novel photoresponsive molecularly imprinted polymer (MIP) was developed for the selective extraction of guanine from complex samples. The photoresponsive MIP was fabricated using guanine as the template, water-soluble 5-[(4-(methacryloyloxy)phenyl)diazenyl]isophthalic acid as the functional monomer, and water-soluble triethanolamine trimethacrylate as the cross-linker. The MIP displayed good selectivity toward guanine with a dissociation constant of (2.70 ± 0.16) × 10(-5) mol L(-1) in aqueous media. The density of the guanine-specific receptor sites in the MIP material was (4.49 ± 0.22)μmol g(-1). Quantitatively release and uptake of guanine by the MIP occurred with irradiation at 365 and 440 nm, respectively. The MIP could efficiently extract guanine from beer and then release it into aqueous media under photocontrol. This method could be used for selective separation and subsequent determination of a specific analytes from complex samples.
authors.affiliation: The Key Laboratory of Applied Chemistry of Chongqing Municipality, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China.
authors.forename: Zai-yong
authors.initials: ZY
authors.lastname: Li
authors.affiliation: The Key Laboratory of Applied Chemistry of Chongqing Municipality, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China.
authors.forename: Hong-jiang
authors.initials: HJ
authors.lastname: Quan
authors.affiliation: The Key Laboratory of Applied Chemistry of Chongqing Municipality, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China. Electronic address: gongcbtq@swu.edu.cn.
authors.forename: Cheng-bin
authors.initials: CB
authors.lastname: Gong
authors.affiliation: The Key Laboratory of Applied Chemistry of Chongqing Municipality, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China.
authors.forename: Yu-zhu
authors.initials: YZ
authors.lastname: Yang
authors.affiliation: The Key Laboratory of Applied Chemistry of Chongqing Municipality, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China. Electronic address: qiantang@swu.edu.cn.
authors.forename: Qian
authors.initials: Q
authors.lastname: Tang
authors.affiliation: The Key Laboratory of Applied Chemistry of Chongqing Municipality, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China.
authors.forename: Yu-bo
authors.initials: YB
authors.lastname: Wei
authors.affiliation: The Key Laboratory of Applied Chemistry of Chongqing Municipality, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China.
authors.forename: Xue-bing
authors.initials: XB
authors.lastname: Ma
authors.affiliation: Department of Biology & Chemistry, City University of Hong Kong, Hong Kong, China.
authors.forename: Hon-wah
authors.initials: HW
authors.lastname: Lam
mesh_terms: D000327:Adsorption / Q000528:radiation effects; D001515:Beer / Q000032:analysis*; D006147:Guanine / Q000737:chemistry / Q000302:isolation & purification*; D054802:Molecular Imprinting; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry*; D052616:Solid Phase Extraction / Q000295:instrumentation / Q000379:methods*
chemical_list: D011108:Polymers; D006147:Guanine
keywords: Adenine; Beer; Guanine; Photoresponsive molecularly imprinted polymer; Solid-phase extraction; Uric acid