title: [SOS repair of 8-methoxypsoralene monoadducts in DNA of lambda bacteriophage and plasmids is mediated by MucA'2B, but not UmuD'2c (PolV) polymerase].
abstract: The light-induced action of 8-methoxypsoralen (8-MOP) on λ phage and plasmids yields monoadducts and interstrand crosslinks. The survival and clear plaque mutation frequency in the phage photosensitized with 8-MOP and irradiated with UV at wavelength > 320 nm are increased whenthe wild-type host (Escherichia coli uvr+) is subjected to UV irradiation (wavelength = 254 nm) prior to phage inoculation. These phenomena are known as "W reactivation" and "W mutagenesis." It is shown that 8-MOP monoadducts in λ DNA in- duce clear mutations in the phage inoculated to UV-irradiated excision repair mutants of E. coli only when the error-prone repair is performed by MucA B, but not PolV (UmuD'2C) polymerase. The efficiency of the SOS repair (W reactivation) of 8-MOP monoadducts in plasmid and λ phage DNA also only increases with the presence of pKM101 plasmid muc+ in E. coli uvr-.
authors.forename: G B
authors.initials: GB
authors.lastname: Zavigel'skii
authors.forename: V Yu
authors.initials: VY
authors.lastname: Kotova
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D010582:Bacteriophage lambda / Q000187:drug effects / Q000235:genetics* / Q000528:radiation effects; D018736:DNA Adducts / Q000235:genetics*; D004259:DNA-Directed DNA Polymerase / Q000235:genetics / Q000378:metabolism*; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics* / Q000528:radiation effects; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism*; D008730:Methoxsalen / Q000528:radiation effects / Q000633:toxicity; D059645:Mutation Rate; D017319:Photosensitizing Agents / Q000528:radiation effects / Q000633:toxicity; D010957:Plasmids / Q000187:drug effects / Q000235:genetics / Q000528:radiation effects; D013014:SOS Response, Genetics; D014466:Ultraviolet Rays
chemical_list: D001426:Bacterial Proteins; D018736:DNA Adducts; D029968:Escherichia coli Proteins; C063757:MucA protein, Bacteria; D017319:Photosensitizing Agents; D004259:DNA-Directed DNA Polymerase; C055218:UmuD protein, E coli; D008730:Methoxsalen