title: Comparison of different twin-screw extraction conditions for the production of arabinoxylans.
abstract: The aim of this article is to compare two different sets of optimal conditions for twin-screw extraction of xylans and define their influence on the purification steps, combining ultrafiltration and industrial chromatography. Two xylan extracts were obtained by twin-screw extrusion of straw and bran. Condition 1 used a high straw/bran ratio (equal to 6) and high sodium hydroxide content, and condition 2 used a lower straw/bran ratio (equal to 2) and low sodium hydroxide content. Arabinoxylan extraction yields are slightly higher for conditions with low straw content (5.1% versus 4.4%). Nevertheless, these recovery yields remain between 9% and 10%. Ultrafiltration is as efficient as evaporation for polysaccharide concentration, with lower energy consumption, but also demineralizes the solution. The combination of ultrafiltration and chromatography gives partial purification of the extract with a final arabinoxylan purity ranging from 16% to 26%. This is slightly higher than by direct precipitation, but limited because all the large molecules such as proteins and lignins were retained by ultrafiltration.
authors.affiliation: Université de Toulouse, INP-ENSIACET, LCA (Laboratoire de Chimie Agro-industrielle), F-31030 Toulouse, France; INRA, UMR 1010 CAI, F-31030 Toulouse, France. Electronic address: leslie.jacquemin@ensiacet.fr.
authors.forename: Leslie
authors.initials: L
authors.lastname: Jacquemin
authors.affiliation: Université de Toulouse, INP-ENSIACET, LCA (Laboratoire de Chimie Agro-industrielle), F-31030 Toulouse, France; INRA, UMR 1010 CAI, F-31030 Toulouse, France.
authors.forename: Assad
authors.initials: A
authors.lastname: Mogni
authors.affiliation: Université de Toulouse, INP-ENSIACET, LCA (Laboratoire de Chimie Agro-industrielle), F-31030 Toulouse, France; INRA, UMR 1010 CAI, F-31030 Toulouse, France.
authors.forename: Rawan
authors.initials: R
authors.lastname: Zeitoun
authors.affiliation: INRA, UR 1268 BIA, F44316 Nantes, France.
authors.forename: Cécile
authors.initials: C
authors.lastname: Guinot
authors.affiliation: Université de Toulouse, INP-ENSIACET, LCA (Laboratoire de Chimie Agro-industrielle), F-31030 Toulouse, France; INRA, UMR 1010 CAI, F-31030 Toulouse, France.
authors.forename: Caroline
authors.initials: C
authors.lastname: Sablayrolles
authors.affiliation: INRA, UR 1268 BIA, F44316 Nantes, France.
authors.forename: Luc
authors.initials: L
authors.lastname: Saulnier
authors.affiliation: Université de Toulouse, INP-ENSIACET, LCA (Laboratoire de Chimie Agro-industrielle), F-31030 Toulouse, France; INRA, UMR 1010 CAI, F-31030 Toulouse, France. Electronic address: pierreyves.pontalier@ensiacet.fr.
authors.forename: Pierre-Yves
authors.initials: PY
authors.lastname: Pontalier
mesh_terms: D002852:Chromatography, Ion Exchange; D012972:Sodium Hydroxide / Q000737:chemistry; D014908:Triticum; D014462:Ultrafiltration; D014990:Xylans / Q000302:isolation & purification*
chemical_list: D014990:Xylans; D012972:Sodium Hydroxide; C085118:arabinoxylan
keywords: Alkali extraction; Arabinoxylans; Destarching; Resin chromatography; Twin-screw extrusion; Ultrafiltration