title: Biodegradable capsules as non-viral vectors for in vitro delivery of PEI/siRNA polyplexes for efficient gene silencing.
abstract: The efficiency of siRNA delivery is demonstrated to be improved by encapsulating the siRNA within a non-viral carrier based on layer-by-layer assembly of oppositely charged biodegradable and biocompatible polyelectrolytes. In comparison to other non-viral delivery vehicles such as polycation-based complexes, a smaller amount of siRNA was necessary to produce in vitro gene silencing as early as 20-30 h after incubation. Colloidal carriers based on assembled biodegradable polyelectrolytes offer several advantages, such as efficient intracellular delivery after endocytosis followed by release to the cytosol, as well as protection of the siRNA, which is crucial for its therapeutic activity.
authors.affiliation: Fachbereich Physik, Philipps-Universität Marburg, 35037 Marburg, Germany.
authors.forename: Carolin
authors.initials: C
authors.lastname: Ganas
authors.affiliation: Fachbereich Physik, Philipps-Universität Marburg, 35037 Marburg, Germany.
authors.forename: Annika
authors.initials: A
authors.lastname: Weiß
authors.affiliation: Fachbereich Physik, Philipps-Universität Marburg, 35037 Marburg, Germany.
authors.forename: Moritz
authors.initials: M
authors.lastname: Nazarenus
authors.affiliation: Fachbereich Pharmazie, Philipps-Universität Marburg, 35037 Marburg, Germany.
authors.forename: Susanne
authors.initials: S
authors.lastname: Rösler
authors.affiliation: Fachbereich Pharmazie, Philipps-Universität Marburg, 35037 Marburg, Germany.
authors.forename: Thomas
authors.initials: T
authors.lastname: Kissel
authors.affiliation: Fachbereich Physik, Philipps-Universität Marburg, 35037 Marburg, Germany. Electronic address: pilar.rivera@urv.cat.
authors.forename: Pilar
authors.initials: P
authors.lastname: Rivera Gil
authors.affiliation: Fachbereich Physik, Philipps-Universität Marburg, 35037 Marburg, Germany; CIC Biomagune, 20009 Donostia-San Sebastián, Spain. Electronic address: wolfgang.parak@physik.uni-marburg.de.
authors.forename: Wolfgang J
authors.initials: WJ
authors.lastname: Parak
mesh_terms: D002214:Capsules; D002470:Cell Survival / Q000187:drug effects; D003102:Colloids; D004339:Drug Compounding; D004705:Endocytosis / Q000187:drug effects; D020868:Gene Silencing / Q000187:drug effects*; D005822:Genetic Vectors / Q000008:administration & dosage*; D049452:Green Fluorescent Proteins / Q000235:genetics; D006367:HeLa Cells; D006801:Humans; D011094:Polyethyleneimine / Q000737:chemistry* / Q000633:toxicity; D034741:RNA, Small Interfering / Q000008:administration & dosage*
chemical_list: D002214:Capsules; D003102:Colloids; D034741:RNA, Small Interfering; D049452:Green Fluorescent Proteins; D011094:Polyethyleneimine
keywords: Dextran sulfate (PubChem SID: 57288723); Gene silencing; Microcapsules; Non-viral vectors; Poly-l-arginine (PubChem CID: 6322); Polyallylamine (Pub Chem CID: 7853); Polyethylenimine (PubChem CID: 9033); Polyplexes; Polystyrene sulfonate (Pub Chem CID: 75905); Transfection; siRNA