title: Metabolic engineering as a tool for enhanced lactic acid production.
abstract: Metabolic engineering is a powerful biotechnological tool that finds, among others, increased use in constructing microbial strains for higher lactic acid productivity, lower costs and reduced pollution. Engineering the metabolic pathways has concentrated on improving the lactic acid fermentation parameters, enhancing the acid tolerance of production organisms and their abilities to utilize a broad range of substrates, including fermentable biomass-derived sugars. Recent efforts have focused on metabolic engineering of lactic acid bacteria as they produce high yields and have a small genome size that facilitates their genetic manipulation. We summarize here the current trends in metabolic engineering techniques and strategies for manipulating lactic acid producing organisms developed to address and overcome major challenges in the lactic acid production process.
authors.affiliation: Center for Bioprocessing Research & Development, South Dakota School of Mines & Technology, Rapid City 57701, SD, USA; Biomedical Engineering Program, South Dakota School of Mines & Technology, Rapid City 57701, SD, USA.
authors.forename: Bikram P
authors.initials: BP
authors.lastname: Upadhyaya
authors.affiliation: Center for Bioprocessing Research & Development, South Dakota School of Mines & Technology, Rapid City 57701, SD, USA; Biomedical Engineering Program, South Dakota School of Mines & Technology, Rapid City 57701, SD, USA; Department of Chemistry & Applied Biological Sciences, South Dakota School of Mines & Technology, Rapid City 57701, SD, USA.
authors.forename: Linda C
authors.initials: LC
authors.lastname: DeVeaux
authors.affiliation: Center for Bioprocessing Research & Development, South Dakota School of Mines & Technology, Rapid City 57701, SD, USA; Biomedical Engineering Program, South Dakota School of Mines & Technology, Rapid City 57701, SD, USA. Electronic address: lew.christopher@sdsmt.edu.
authors.forename: Lew P
authors.initials: LP
authors.lastname: Christopher
mesh_terms: D002241:Carbohydrates / Q000032:analysis; D003600:Cytosol / Q000737:chemistry; D019344:Lactic Acid / Q000378:metabolism*; D056546:Lactobacillales / Q000235:genetics* / Q000378:metabolism*; D060847:Metabolic Engineering / Q000379:methods* / Q000639:trends; D053858:Metabolic Networks and Pathways / Q000235:genetics
chemical_list: D002241:Carbohydrates; D019344:Lactic Acid
keywords: acid tolerance; carbon source; fermentation parameters; lactic acid production; lactic acid purity; metabolic engineering