title: One step in-situ synthesis of amine functionalized graphene for immunosensing of cardiac marker cTnI.
abstract: 2-Aminobenzyl amine (2-ABA) functionalized graphene is proposed for the ultrasensitive immunosensing of Cardiac Troponin I (cTnI). 2-ABA was electrochemically polymerized on the graphene decorated interdigitated electrode to obtain the amine functionalized graphene (f-GN). The f-GN electrode was then modified with monoclonal anti-cTnI antibodies via Schiff reaction based chemistry. Detailed characteristics of the processes involved and the finally developed antibody conjugated f-GN interdigitated electrode have been studied. The above micro-device was used in a drain source configuration for the sensing of cTnI. A wide dynamic linear range of antigen detection (0.01-1ng/mL) is achieved with the limit of detection of 0.01ng/mL. The utility of the proposed sensing technique is demonstrated by successfully testing the antigen concentration in spiked serum samples.
authors.affiliation: Academy of Innovative and Scientific Research (AcSIR-CSIO), Chandigarh 160030, India; CSIR-Central Scientific Instruments Organization (CSIO), Chandigarh 160030, India; CSIR-Institute of Microbial Technology (IMTECH), Chandigarh 160036, India.
authors.forename: Satish K
authors.initials: SK
authors.lastname: Tuteja
authors.affiliation: Academy of Innovative and Scientific Research (AcSIR-CSIO), Chandigarh 160030, India; CSIR-Central Scientific Instruments Organization (CSIO), Chandigarh 160030, India.
authors.forename: Manil
authors.initials: M
authors.lastname: Kukkar
authors.affiliation: CSIR-Institute of Microbial Technology (IMTECH), Chandigarh 160036, India. Electronic address: raman@imtech.res.in.
authors.forename: C R
authors.initials: CR
authors.lastname: Suri
authors.affiliation: Academy of Innovative and Scientific Research (AcSIR-CSIO), Chandigarh 160030, India; CSIR-Central Scientific Instruments Organization (CSIO), Chandigarh 160030, India.
authors.forename: A K
authors.initials: AK
authors.lastname: Paul
authors.affiliation: Academy of Innovative and Scientific Research (AcSIR-CSIO), Chandigarh 160030, India; CSIR-Central Scientific Instruments Organization (CSIO), Chandigarh 160030, India. Electronic address: dr.akashdeep@gmail.com.
authors.forename: Akash
authors.initials: A
authors.lastname: Deep
mesh_terms: D055910:Antibodies, Immobilized / Q000737:chemistry*; D001596:Benzylamines / Q000138:chemical synthesis / Q000737:chemistry*; D015415:Biomarkers / Q000032:analysis / Q000097:blood; D015374:Biosensing Techniques / Q000295:instrumentation; D055664:Electrochemical Techniques / Q000295:instrumentation*; D004566:Electrodes; D004867:Equipment Design; D006108:Graphite / Q000138:chemical synthesis / Q000737:chemistry*; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation*; D057230:Limit of Detection; D019210:Troponin I / Q000032:analysis / Q000097:blood*
chemical_list: D055910:Antibodies, Immobilized; D001596:Benzylamines; D015415:Biomarkers; D019210:Troponin I; D006108:Graphite
keywords: 2-Aminobenzyl amine; Biomarker; Cardiac Troponin I; Graphene; Immunosensor