title: Contrasting stress responses of two co-occurring chipmunk species (Tamias alpinus and T. speciosus).
abstract: Glucocorticoid (GC) hormones are important mediators of responses to environmental conditions. Accordingly, differences in GC physiology may contribute to interspecific variation in response to anthropogenically-induced patterns of climate change. To begin exploring this possibility, we validated the use of fecal cortisol/corticosterone metabolites (FCM) to measure baseline glucocorticoid levels in two species of co-occurring chipmunks that have exhibited markedly different patterns of response to environmental change. In Yosemite National Park, the alpine chipmunk (Tamias alpinus) has undergone a significant upward contraction of its elevational range over the past century; in contrast, the lodgepole chipmunk (Tamiasspeciosus) has experienced no significant change in elevational distribution over this period. To determine if GC levels in these species vary in response to external stimuli and to assess whether these responses differ between species, we compared FCM levels for the same individuals (1) at the time of capture in the field, (2) after a short period of captivity, and (3) after adrenocorticotropic hormone (ACTH), (4) handling, and (5) trapping challenges conducted while these animals were held in captivity. Our analyses indicate that T. alpinus was more responsive to several of these changes in external conditions. Although both species displayed a significant FCM response to ACTH challenge, only T. alpinus showed a significant response to our handling challenge and to captive housing conditions. These findings underscore the importance of species-specific validation studies and support the potential for studies of GC physiology to generate insights into interspecific differences in response to environmental change.
authors.affiliation: Department of Integrative Biology, 1001 Valley Life Sciences Building, University of California Berkeley, Berkeley, CA 94720-3160, USA; Museum of Vertebrate Zoology, 3101 Valley Life Sciences Building, University of California Berkeley, Berkeley, CA 94720-3160, USA. Electronic address: thammond@berkeley.edu.
authors.forename: Talisin T
authors.initials: TT
authors.lastname: Hammond
authors.affiliation: Institute of Medical Biochemistry, Department of Biomedical Sciences, University of Veterinary Medicine, Veterinärplatz 1, 1210 Vienna, Austria.
authors.forename: Rupert
authors.initials: R
authors.lastname: Palme
authors.affiliation: Department of Integrative Biology, 1001 Valley Life Sciences Building, University of California Berkeley, Berkeley, CA 94720-3160, USA; Museum of Vertebrate Zoology, 3101 Valley Life Sciences Building, University of California Berkeley, Berkeley, CA 94720-3160, USA.
authors.forename: Eileen A
authors.initials: EA
authors.lastname: Lacey
mesh_terms: D000324:Adrenocorticotropic Hormone / Q000494:pharmacology; D000818:Animals; D002140:California; D002940:Circadian Rhythm / Q000187:drug effects; D057231:Climate Change; D003345:Corticosterone / Q000378:metabolism; D005243:Feces / Q000737:chemistry; D005260:Female; D006234:Handling, Psychological; D006854:Hydrocortisone / Q000378:metabolism; D008297:Male; D012589:Sciuridae / Q000502:physiology*; D013045:Species Specificity; D013312:Stress, Physiological / Q000187:drug effects / Q000502:physiology*
chemical_list: D000324:Adrenocorticotropic Hormone; D003345:Corticosterone; D006854:Hydrocortisone
keywords: Chipmunks; Comparative physiology; Fecal glucocorticoid metabolites; Handling stressor; Interspecific variation; Stress