title: Neurogenesis of the spiral ganglion cells in the cochlea requires the transcriptional cofactor TIS21.
abstract: The molecular mechanisms controlling the proliferation and differentiation of spiral ganglion cells (SGCs) in the inner ear are still largely unknown. TIS21 is a transcriptional cofactor that shows antiproliferative, antiapoptotic, and prodifferentiative effects on neural progenitor cells. To investigate the function of TIS21 during SGC development, we analyzed SGC neurogenesis from embryonic day 13.5 (E13.5) to postnatal day 4 (P4) in Tis21-GFP knock-in mice, in which the protein-encoding exon of the Tis21 gene was replaced by EGFP. Through E13.5 to P4, we found fewer SGCs in homozygous Tis21-GFP knock-in mice than in wild-type mice. Our results suggest that TIS21 is required for development of SGCs. Deleting Tis21 may affect progenitor cells or neuroblasts at the beginning of cochlear-vestibular ganglion formation and would consequently lead to a decrease in the number of SGCs.
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, Kumamoto University, Graduate School of Medicine, 1-1-1 Honjo Chuouku, Kumamoto city, Kumamoto 860-8556, Japan.
authors.forename: Takao
authors.initials: T
authors.lastname: Yamada
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, Kumamoto University, Graduate School of Medicine, 1-1-1 Honjo Chuouku, Kumamoto city, Kumamoto 860-8556, Japan. Electronic address: minoda@gpo.kumamoto-u.ac.jp.
authors.forename: Ryosei
authors.initials: R
authors.lastname: Minoda
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, Kumamoto University, Graduate School of Medicine, 1-1-1 Honjo Chuouku, Kumamoto city, Kumamoto 860-8556, Japan.
authors.forename: Toru
authors.initials: T
authors.lastname: Miwa
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, Kumamoto University, Graduate School of Medicine, 1-1-1 Honjo Chuouku, Kumamoto city, Kumamoto 860-8556, Japan.
authors.forename: Momoko
authors.initials: M
authors.lastname: Ise
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, Kumamoto University, Graduate School of Medicine, 1-1-1 Honjo Chuouku, Kumamoto city, Kumamoto 860-8556, Japan.
authors.forename: Hiroki
authors.initials: H
authors.lastname: Takeda
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, Kumamoto University, Graduate School of Medicine, 1-1-1 Honjo Chuouku, Kumamoto city, Kumamoto 860-8556, Japan.
authors.forename: Eiji
authors.initials: E
authors.lastname: Yumoto
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D002452:Cell Count; D055879:Gene Knock-In Techniques; D006720:Homozygote; D017874:Immediate-Early Proteins / Q000235:genetics / Q000378:metabolism*; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D055495:Neurogenesis; D013136:Spiral Ganglion / Q000166:cytology / Q000196:embryology* / Q000254:growth & development*; D025521:Tumor Suppressor Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: C489435:Btg2 protein, mouse; D017874:Immediate-Early Proteins; D025521:Tumor Suppressor Proteins
keywords: Cochlea; Development; Inner ear; Neurogenesis; Spiral ganglion cell; TIS21