title: Aqueous two-phase extraction for determination of triazine herbicides in milk by high-performance liquid chromatography.
abstract: A simple extraction method based on acetonitrile-K2HPO4 aqueous two-phase system was developed for separation and enrichment of five triazines in milk samples. Acetonitrile was used for extraction of analytes from milk sample and precipitation of milk protein. Deproteinization and extraction were achieved in one single step. Analytes were extracted into the upper phase of the aqueous two-phase system. The parameters affecting the extraction efficiency, such as the volume of acetonitrile, the type and amount of salts, pH value of sample and extraction time were investigated. The limits of detection of atraton, desmetryn, atrazine, terbumeton and terbuthylazine were 2.1, 2.6, 2.3, 2.8 and 2.5μg/L, respectively. When the present method was applied to the analysis of real milk samples, the recoveries of analytes ranged from 86.3 to 120.6% and relative standard deviations were lower than 7.9%.
authors.affiliation: College of Chemistry, Jilin University, Qianjin Street 2699, Changchun 130012, PR China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Yang
authors.affiliation: College of Chemistry, Jilin University, Qianjin Street 2699, Changchun 130012, PR China.
authors.forename: Rui
authors.initials: R
authors.lastname: Yu
authors.affiliation: Department of Rehabilitation, the General Hospital of FAW (the Fourth Hospital of Jilin University), Haoyue Road 3788, Changchun 130011, PR China.
authors.forename: Shaohua
authors.initials: S
authors.lastname: Zhang
authors.affiliation: College of Chemistry, Jilin University, Qianjin Street 2699, Changchun 130012, PR China.
authors.forename: Bocheng
authors.initials: B
authors.lastname: Cao
authors.affiliation: College of Chemistry, Jilin University, Qianjin Street 2699, Changchun 130012, PR China.
authors.forename: Zhongling
authors.initials: Z
authors.lastname: Liu
authors.affiliation: College of Chemistry, Jilin University, Qianjin Street 2699, Changchun 130012, PR China.
authors.forename: Lei
authors.initials: L
authors.lastname: Lei
authors.affiliation: College of Chemistry, Jilin University, Qianjin Street 2699, Changchun 130012, PR China.
authors.forename: Na
authors.initials: N
authors.lastname: Li
authors.affiliation: College of Chemistry, Jilin University, Qianjin Street 2699, Changchun 130012, PR China.
authors.forename: Zhibing
authors.initials: Z
authors.lastname: Wang
authors.affiliation: College of Chemistry, Jilin University, Qianjin Street 2699, Changchun 130012, PR China.
authors.forename: Liyuan
authors.initials: L
authors.lastname: Zhang
authors.affiliation: College of Chemistry, Jilin University, Qianjin Street 2699, Changchun 130012, PR China.
authors.forename: Hanqi
authors.initials: H
authors.lastname: Zhang
authors.affiliation: College of Chemistry, Jilin University, Qianjin Street 2699, Changchun 130012, PR China. Electronic address: vivianyxiao@163.com.
authors.forename: Yanhua
authors.initials: Y
authors.lastname: Chen
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006540:Herbicides / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D057230:Limit of Detection; D008892:Milk / Q000737:chemistry*; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet; D014227:Triazines / Q000032:analysis*
chemical_list: D006540:Herbicides; D014227:Triazines
keywords: Aqueous two-phase extraction; Milk; Triazine herbicides