title: Complete genome characterization and phylogenetic analysis of three distinct buffalo-origin PCV2 isolates from China.
abstract: Complete genome characterization of porcine circovirus type 2 (PCV2) for bovid origins was still unclear in China. Therefore, in this study, PCV2 full-length genome of buffalo-origin was amplified and analyzed using PCR, DNAStar and MEGA 5.1. Genome size of three distinct PCV2 strains (buffalo1, buffalo2 and buffalo3) was 1767 bp (48.56% G+C), 1767 bp (48.67% G+C) and 1768 bp (48.08% G+C), respectively. At the nucleotide level, their identity varied from 95% to 96% for complete genome, from 97% to 97.8% for ORF1, and from 90.6% to 94.4% for ORF2. At the amino acid level, their identity varied from 98.7% to 99% for ORF1, and from 88% to 94.9% for ORF2. Online Blast analysis showed that buffalo1, buffalo2 and buffalo3 had highest nucleotide identity (varied from 99.77% to 99.83%) with porcine-origin PCV2 strains. Moreover, in the phylogenetic tree, they were divided into three different clusters and belonged to the worldwide accepted genotypes of PCV2b, PCV2c and PCV2a, respectively. To summarize, this study first recorded complete genome information of PCV2 for non-porcine origins in China.
authors.affiliation: College of Veterinary Medicine, South China Agricultural University, No. 483 Wushan Road, Tianhe District, Guangzhou 510642, China; Institute of Animal Health, Guangdong Academy of Agricultural Sciences, No. 21 Baishigang Street, Wushan Road, Tianhe District, Guangzhou 510640, China.
authors.forename: Shao-Lun
authors.initials: SL
authors.lastname: Zhai
authors.affiliation: College of Veterinary Medicine, South China Agricultural University, No. 483 Wushan Road, Tianhe District, Guangzhou 510642, China; Guangdong Dahuanong Animal Health Products Co., Ltd., Xinxing 527400, China.
authors.forename: Dong-Sheng
authors.initials: DS
authors.lastname: He
authors.affiliation: College of Veterinary Medicine, South China Agricultural University, No. 483 Wushan Road, Tianhe District, Guangzhou 510642, China.
authors.forename: Wen-Bao
authors.initials: WB
authors.lastname: Qi
authors.affiliation: Guangdong Dahuanong Animal Health Products Co., Ltd., Xinxing 527400, China.
authors.forename: Sheng-Nan
authors.initials: SN
authors.lastname: Chen
authors.affiliation: College of Veterinary Medicine, South China Agricultural University, No. 483 Wushan Road, Tianhe District, Guangzhou 510642, China.
authors.forename: Su-Fang
authors.initials: SF
authors.lastname: Deng
authors.affiliation: College of Veterinary Medicine, South China Agricultural University, No. 483 Wushan Road, Tianhe District, Guangzhou 510642, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Hu
authors.affiliation: College of Veterinary Medicine, South China Agricultural University, No. 483 Wushan Road, Tianhe District, Guangzhou 510642, China.
authors.forename: Xiao-Peng
authors.initials: XP
authors.lastname: Li
authors.affiliation: Henan Entry-Exit Inspection and Quarantine Bureau, No. 269 Jinshui Road, Jinshui District, Zhengzhou 450003, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Li
authors.affiliation: College of Veterinary Medicine, South China Agricultural University, No. 483 Wushan Road, Tianhe District, Guangzhou 510642, China; Guangdong Dahuanong Animal Health Products Co., Ltd., Xinxing 527400, China.
authors.forename: Rui-Ai
authors.initials: RA
authors.lastname: Chen
authors.affiliation: College of Veterinary Medicine, South China Agricultural University, No. 483 Wushan Road, Tianhe District, Guangzhou 510642, China; Guangdong Dahuanong Animal Health Products Co., Ltd., Xinxing 527400, China. Electronic address: luoml@scau.edu.cn.
authors.forename: Man-Lin
authors.initials: ML
authors.lastname: Luo
authors.affiliation: Institute of Animal Health, Guangdong Academy of Agricultural Sciences, No. 21 Baishigang Street, Wushan Road, Tianhe District, Guangzhou 510640, China. Electronic address: wwk188@tom.com.
authors.forename: Wen-Kang
authors.initials: WK
authors.lastname: Wei
mesh_terms: D000818:Animals; D002020:Buffaloes / Q000821:virology*; D002681:China; D018173:Circoviridae Infections / Q000662:veterinary / Q000821:virology*; D017925:Circovirus / Q000145:classification* / Q000235:genetics*; D016679:Genome, Viral / Q000235:genetics; D006801:Humans; D010802:Phylogeny; D013552:Swine / Q000821:virology
keywords: Buffalo-origin; Genetic diversity; Genome characterization; Phylogenetic analysis; Porcine circovirus type 2