title: Treatment of textile effluent in a developed phytoreactor with immobilized bacterial augmentation and subsequent toxicity studies on Etheostoma olmstedi fish.
abstract: A static hydroponic bioreactor using nursery grown plants of Pogonatherum crinitum along with immobilized Bacillus pumilus cells was developed for the treatment of textile wastewater. Independent reactors with plants and immobilized cells were also kept for performance and efficacy evaluation. The effluent samples characterized before and after their treatment showed that the plant-bacterial consortium reactor was more efficient than those of individual plant and bacterium reactors. COD, BOD, ADMI, conductivity, turbidity, TDS and TSS of the textile effluent was found to be reduced by 78, 70, 93, 4, 90, 13 and 70% respectively within 12 d by the consortial set. HPTLC analysis revealed the transformation of the textile effluent to new products. The phytotoxicity study on Phaeseolus mungo and Sorghum vulgare seeds showed reduced toxicity of treated effluents. The animal toxicity study performed on Etheostoma olmstedi fishes showed the toxic nature of untreated effluent giving extreme stress to fishes leading to death. Histology of fish gills exposed to treated effluent was found to be less affected. The oxidative stress related enzymes like superoxide dismutase and catalase were found to show decreased activities and less lipid peroxidation in fishes exposed to treated effluent.
authors.affiliation: Department of Biotechnology, Shivaji University, Kolhapur, India.
authors.forename: Anuprita D
authors.initials: AD
authors.lastname: Watharkar
authors.affiliation: School of Life Sciences, North Maharashtra University, Jalgaon, India.
authors.forename: Rahul V
authors.initials: RV
authors.lastname: Khandare
authors.affiliation: Department of Biochemistry, Shivaji University, Kolhapur, India.
authors.forename: Pankajkumar R
authors.initials: PR
authors.lastname: Waghmare
authors.affiliation: Department of Biochemistry, Shivaji University, Kolhapur, India.
authors.forename: Ashwini D
authors.initials: AD
authors.lastname: Jagadale
authors.affiliation: Department of Biochemistry, Shivaji University, Kolhapur, India.
authors.forename: Sanjay P
authors.initials: SP
authors.lastname: Govindwar
authors.affiliation: Department of Biotechnology, Shivaji University, Kolhapur, India; Department of Biochemistry, Shivaji University, Kolhapur, India. Electronic address: jpj_biochem@unishivaji.ac.in.
authors.forename: Jyoti P
authors.initials: JP
authors.lastname: Jadhav
mesh_terms: D000818:Animals; D001407:Bacillus / Q000378:metabolism*; D001673:Biodegradation, Environmental; D019149:Bioreactors / Q000382:microbiology*; D002374:Catalase / Q000378:metabolism; D018914:Cells, Immobilized / Q000382:microbiology; D004396:Coloring Agents / Q000737:chemistry / Q000633:toxicity; D005880:Gills / Q000187:drug effects / Q000473:pathology; D007220:Industrial Waste; D015227:Lipid Peroxidation; D018384:Oxidative Stress; D010473:Perciformes; D027805:Phaseolus / Q000187:drug effects; D006109:Poaceae / Q000378:metabolism*; D045868:Sorghum / Q000187:drug effects; D013482:Superoxide Dismutase / Q000378:metabolism; D013783:Textile Industry; D014865:Waste Disposal, Fluid / Q000379:methods*; D062065:Wastewater / Q000737:chemistry* / Q000633:toxicity
chemical_list: D002374:Catalase; D004396:Coloring Agents; D007220:Industrial Waste; D013482:Superoxide Dismutase; D062065:Wastewater
keywords: Decolorization; E. olmstedi; Oxidative stress; P. crinitum; Phytoreactor; Phytoremediation