title: BMP signaling is required for cell cleavage in preimplantation-mouse embryos.
abstract: The mechanisms regulating cell division during development of the mouse pre-implantation embryo are poorly understood. We have investigated whether bone morphogenetic protein (BMP) signaling is involved in controlling cell cycle during mouse pre-implantation development. We mapped and quantitated the dynamic activities of BMP signaling through high-resolution immunofluorescence imaging combined with a 3D segmentation method. Immunostaining for phosphorylated Smad1/5/8 shows that BMP signaling is activated in mouse embryos as early as the 4-cell stage, and becomes spatially restricted by late blastocyst stage. Perturbation of BMP signaling in preimplantation mouse embryos, whether by treatment with a small molecule inhibitor, with Noggin protein, or by overexpression of a dominant-negative BMP receptor, indicates that BMPs regulate cell cleavage up to the morula stage. These results indicate that BMP signaling is active during mouse pre-implantation development and is required for cell cleavage in preimplantation mouse embryos.
authors.affiliation: Department of Developmental & Cell Biology, University of California, Irvine, CA 92697-2300 USA.
authors.forename: Nabora Soledad
authors.initials: NS
authors.lastname: Reyes de Mochel
authors.affiliation: Department of Developmental & Cell Biology, University of California, Irvine, CA 92697-2300 USA.
authors.forename: Mui
authors.initials: M
authors.lastname: Luong
authors.affiliation: Department of Developmental & Cell Biology, University of California, Irvine, CA 92697-2300 USA.
authors.forename: Michael
authors.initials: M
authors.lastname: Chiang
authors.affiliation: Department of Developmental & Cell Biology, University of California, Irvine, CA 92697-2300 USA.
authors.forename: Anna L
authors.initials: AL
authors.lastname: Javier
authors.affiliation: Department of Developmental & Cell Biology, University of California, Irvine, CA 92697-2300 USA.
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Luu
authors.affiliation: Division of Embryology, National Institute for Basic Biology, Aichi, Japan.
authors.forename: Fujimori
authors.initials: F
authors.lastname: Toshihiko
authors.affiliation: Department of Developmental & Cell Biology, University of California, Irvine, CA 92697-2300 USA.
authors.forename: Grant R
authors.initials: GR
authors.lastname: MacGregor
authors.affiliation: Department of Developmental & Cell Biology, University of California, Irvine, CA 92697-2300 USA.
authors.forename: Olivier
authors.initials: O
authors.lastname: Cinquin
authors.affiliation: Department of Developmental & Cell Biology, University of California, Irvine, CA 92697-2300 USA.
authors.forename: Ken W Y
authors.initials: KW
authors.lastname: Cho
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000502:physiology*; D019485:Bone Morphogenetic Proteins / Q000378:metabolism*; D002352:Carrier Proteins / Q000378:metabolism; D002455:Cell Division; D002478:Cells, Cultured; D047108:Embryonic Development / Q000235:genetics; D053595:Embryonic Stem Cells / Q000166:cytology; D005260:Female; D018507:Gene Expression Regulation, Developmental; D005799:Genes, Dominant; D051379:Mice; D008822:Mice, Transgenic; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D010766:Phosphorylation; D015398:Signal Transduction; D051898:Smad1 Protein / Q000502:physiology; D051902:Smad5 Protein / Q000502:physiology; D051904:Smad8 Protein / Q000502:physiology; D013997:Time Factors; D000099176:Noggin Protein
chemical_list: D019485:Bone Morphogenetic Proteins; D002352:Carrier Proteins; D051898:Smad1 Protein; D051902:Smad5 Protein; D051904:Smad8 Protein; D000099176:Noggin Protein; C497401:Smad1 protein, mouse; C497798:Smad5 protein, mouse; C491621:Smad9 protein, mouse
keywords: BMP; Cell cleavage; Cell division; Morula; Preimplantation; Smad1