title: Improved bioavailability of vitamin D3 using a β-lactoglobulin-based coagulum.
abstract: Vitamin D3 (D3) was encapsulated within a water-soluble matrix, formed by promoting the βlg/D3 complex by acidification. The capacity of the βlg-based coagulum to increase the long term stability of D3 in cold storage, upon exposure to intensive UV-light, and in the presence and absence of intestinal proteases, was evaluated. Additionally, the impact of the sequestration of D3 within the matrix of βlg-based coagulum on its bioavailability was determined in vivo with force-fed rats. The water solubility, long-term storage and UV-light stability of D3 were significantly increased (p < 0.0001) due to the high encapsulation efficiency (94.5 ± 1.8%). The βlg-based coagulum was not rapidly disrupted by the proteases in the intestines, leading to a slow release of D3, increased uptake of D3 and subsequent enhancement of the bioavailability of D3 in rats.
authors.affiliation: Chaire de recherche du Canada sur les protéines, les bio-systèmes et les aliments fonctionnels, Institut de recherche sur les nutraceutiques et les aliments fonctionnels (INAF/STELA), Université Laval, Québec, QC, Canada.
authors.forename: Fatoumata
authors.initials: F
authors.lastname: Diarrassouba
authors.affiliation: EA-CIDAM, Lab Biopharmacie, Faculté de Pharmacie, 28 Place Henri Dunant, 63001 Clermont-Ferrand, France.
authors.forename: Ghislain
authors.initials: G
authors.lastname: Garrait
authors.affiliation: Centre de recherche et développement, Agropur Coopérative, 4700 rue Armand Frappier, St-Hubert, QC J3Z 1G5, Canada.
authors.forename: Gabriel
authors.initials: G
authors.lastname: Remondetto
authors.affiliation: Chaire de recherche du Canada sur les protéines, les bio-systèmes et les aliments fonctionnels, Institut de recherche sur les nutraceutiques et les aliments fonctionnels (INAF/STELA), Université Laval, Québec, QC, Canada.
authors.forename: Pedro
authors.initials: P
authors.lastname: Alvarez
authors.affiliation: EA-CIDAM, Lab Biopharmacie, Faculté de Pharmacie, 28 Place Henri Dunant, 63001 Clermont-Ferrand, France.
authors.forename: Eric
authors.initials: E
authors.lastname: Beyssac
authors.affiliation: Chaire de recherche du Canada sur les protéines, les bio-systèmes et les aliments fonctionnels, Institut de recherche sur les nutraceutiques et les aliments fonctionnels (INAF/STELA), Université Laval, Québec, QC, Canada. Electronic address: Muriel.Subirade@fsaa.ulaval.ca.
authors.forename: Muriel
authors.initials: M
authors.lastname: Subirade
mesh_terms: D000818:Animals; D001682:Biological Availability; D002762:Cholecalciferol / Q000737:chemistry / Q000493:pharmacokinetics*; D004355:Drug Stability; D007782:Lactoglobulins / Q000378:metabolism*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D012995:Solubility
chemical_list: D007782:Lactoglobulins; D002762:Cholecalciferol
keywords: Bioavailability; Encapsulation; Solubility; Stability; Vitamin D(3); β-Lactoglobulin