title: Knockdown of prohibitin expression promotes glucose metabolism in eutopic endometrial stromal cells from women with endometriosis.
abstract: In this in-vitro study, the effect of prohibitin (PHB) on glucose metabolism in eutopic endometrial stromal cells from women with endometriosis was investigated. Endometrial stromal cells were isolated from endometrium in women with endometriosis, in women without endometriosis, or from endometrioma tissues. Glucose metabolic phenotype of stromal cells were examined in vitro. Quantitative polymerase chain reaction was used to measure the mRNA expression of glycolysis-related genes. Glucose consumption and lactate production were examined after knockdown of PHB expression in women with endometriosis with siRNA. In endometrioma tissue, significantly increased glucose consumption, lactate production and aberrant expression of glycolysis-related enzymes were found in women with endometriosis compared with women who do not have endometriosis (P < 0.05 versus P < 0.001). In women with endometriosis, PHB mRNA and protein were under-expressed in endometrioma tissue; in women without endometriosis, PHB mRNA and protein were over-expressed. Knockdown of PHB expression in women with endometriosis increased glucose consumption, although it had no effect on lactate production. This study suggests that aberrant expression of glycolysis-related enzymes in endometrioma tissue is associated with enhanced glycolytic metabolism. The malignant-like feature may be partially caused by low-expression of PHB gene in endometriotic stromal cells.
authors.affiliation: Center of Clinical Reproductive Medicine, State Key Laboratory of Reproductive Medicine, The First Affiliated Hospital of Nanjing Medical University/Jiangsu Province Hospital, Nanjing, China; Department of Obstetrics and Gynecology, The First Clinical Medical College of Three Gorges University, Yichang Central People's Hospital, Yichang, China.
authors.forename: Xiaochen
authors.initials: X
authors.lastname: Qi
authors.affiliation: Center of Clinical Reproductive Medicine, State Key Laboratory of Reproductive Medicine, The First Affiliated Hospital of Nanjing Medical University/Jiangsu Province Hospital, Nanjing, China; Department of Obstetrics and Gynecology, Clinical Medical College of Yangzhou University, Subei People's Hospital of Jiangsu, Yangzhou, China.
authors.forename: Yanxin
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Center of Clinical Reproductive Medicine, State Key Laboratory of Reproductive Medicine, The First Affiliated Hospital of Nanjing Medical University/Jiangsu Province Hospital, Nanjing, China; Department of Obstetrics and Gynecology, Nanjing Maternal and Child Health Hospital, Nanjing Medical University, Nanjing, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Ji
authors.affiliation: Center of Clinical Reproductive Medicine, State Key Laboratory of Reproductive Medicine, The First Affiliated Hospital of Nanjing Medical University/Jiangsu Province Hospital, Nanjing, China.
authors.forename: Xiadi
authors.initials: X
authors.lastname: Wu
authors.affiliation: Center of Reproductive and Genetic Medicine, Affiliated Suzhou Hospital of Nanjing Medical University, Suzhou Municipal Hospital, Suzhou, China.
authors.forename: Fuxin
authors.initials: F
authors.lastname: Wang
authors.affiliation: Department of Obstetrics and Gynecology, The First Affiliated Hospital of Nanjing Medical University/Jiangsu Province Hospital, Nanjing, China.
authors.forename: Manxin
authors.initials: M
authors.lastname: Xie
authors.affiliation: Center of Clinical Reproductive Medicine, State Key Laboratory of Reproductive Medicine, The First Affiliated Hospital of Nanjing Medical University/Jiangsu Province Hospital, Nanjing, China.
authors.forename: Li
authors.initials: L
authors.lastname: Shu
authors.affiliation: Department of Biological Science, Mercer University School of Medicine; Savannah, USA; Department of Obstetrics and Gynecology, Memorial Health Hospital, Savannah, USA.
authors.forename: Shiwen
authors.initials: S
authors.lastname: Jiang
authors.affiliation: Center of Clinical Reproductive Medicine, State Key Laboratory of Reproductive Medicine, The First Affiliated Hospital of Nanjing Medical University/Jiangsu Province Hospital, Nanjing, China. Electronic address: drmaoyd@aliyun.com.
authors.forename: Yundong
authors.initials: Y
authors.lastname: Mao
authors.affiliation: Center of Clinical Reproductive Medicine, State Key Laboratory of Reproductive Medicine, The First Affiliated Hospital of Nanjing Medical University/Jiangsu Province Hospital, Nanjing, China.
authors.forename: Yugui
authors.initials: Y
authors.lastname: Cui
authors.affiliation: Center of Clinical Reproductive Medicine, State Key Laboratory of Reproductive Medicine, The First Affiliated Hospital of Nanjing Medical University/Jiangsu Province Hospital, Nanjing, China.
authors.forename: Jiayin
authors.initials: J
authors.lastname: Liu
mesh_terms: D000328:Adult; D004715:Endometriosis / Q000378:metabolism*; D004717:Endometrium / Q000166:cytology*; D005260:Female; D055785:Gene Knockdown Techniques; D005947:Glucose / Q000378:metabolism*; D006801:Humans; D066298:In Vitro Techniques; D019344:Lactic Acid; D000091402:Prohibitins; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D012097:Repressor Proteins / Q000378:metabolism*; D017154:Stromal Cells / Q000378:metabolism*
chemical_list: C000717328:PHB protein, human; D000091402:Prohibitins; D034741:RNA, Small Interfering; D012097:Repressor Proteins; D019344:Lactic Acid; D005947:Glucose
keywords: endometriosis; glucose metabolism; glycolysis; prohibitin