title: Hepatic oxidative status and metal homeostasis disturbance of 2-hydroxylated dioxin in ICR mice.
abstract: In the present study, the toxic effects of the oral exposure of 2-hydroxylated dioxin (2-HODD) in ICR male mice were examined. The mice were administered different doses (0.2, 2.0 and 20.0mg/kg) of 2-HODD. After 14 days of exposure, the oxidative stress (OS) indicator levels and the essential metal concentrations in the mouse livers were determined. The results showed that the superoxide dismutase (SOD) and the glutathione peroxidase (GPx) activities were increased in the 0.2mg/kg group, whereas they were significantly decreased in the 2.0 and 20.0mg/kg groups. Decreases in the catalase (CAT) activity and the glutathione (GSH) levels, accompanied by increases in the malondialdehyde (MDA) contents, were recorded in all of the 2-HODD-treated groups. The hepatic iron, copper and zinc levels increased in all of the 2-HODD-treated groups. The histological examination of the livers demonstrated swollen cells and inflammation. Dose-dependent changes in both the OS indicators and the hepatic metal levels were observed. In conclusion, a single low dose of 2-HODD significantly perturbed the hepatic OS status and metals homeostasis in the mice.
authors.affiliation: State Key Laboratory of Pollution Control and Resources Reuse, School of the Environment, Nanjing University, Nanjing 210023, China.
authors.forename: Xuesheng
authors.initials: X
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Pollution Control and Resources Reuse, School of the Environment, Nanjing University, Nanjing 210023, China.
authors.forename: Li
authors.initials: L
authors.lastname: Qin
authors.affiliation: School of Environmental and Safety Engineering, Changzhou University, Changzhou 213164, China.
authors.forename: Dinglong
authors.initials: D
authors.lastname: Li
authors.affiliation: State Key Laboratory of Pollution Control and Resources Reuse, School of the Environment, Nanjing University, Nanjing 210023, China.
authors.forename: Zhongbo
authors.initials: Z
authors.lastname: Wei
authors.affiliation: State Key Laboratory of Pollution Control and Resources Reuse, School of the Environment, Nanjing University, Nanjing 210023, China. Electronic address: wangzun315cn@163.com.
authors.forename: Zunyao
authors.initials: Z
authors.lastname: Wang
mesh_terms: D000818:Animals; D003300:Copper / Q000378:metabolism*; D004146:Dioxanes / Q000009:adverse effects* / Q000138:chemical synthesis / Q000494:pharmacology; D004147:Dioxins / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D004785:Environmental Pollutants / Q000633:toxicity*; D005786:Gene Expression Regulation / Q000187:drug effects; D005979:Glutathione Peroxidase / Q000378:metabolism; D006706:Homeostasis / Q000187:drug effects; D006900:Hydroxylation; D007501:Iron / Q000378:metabolism*; D008099:Liver / Q000737:chemistry / Q000473:pathology*; D008297:Male; D008315:Malondialdehyde; D051379:Mice; D008813:Mice, Inbred ICR; D018384:Oxidative Stress / Q000187:drug effects; D013482:Superoxide Dismutase / Q000378:metabolism; D015032:Zinc / Q000378:metabolism*
chemical_list: D004146:Dioxanes; D004147:Dioxins; D004785:Environmental Pollutants; D008315:Malondialdehyde; D003300:Copper; D007501:Iron; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D015032:Zinc
keywords: 2-Hydroxylated dibenzo-p-dioxin; Biomarker; Dose-dependent effect; Metal homeostasis; Oxidative stress