title: Affinity chromatography revealed insights into unique functionality of two 14-3-3 protein species in developing maize kernels.
abstract: The 14-3-3 proteins are a group of regulatory proteins of divergent functions in plants. However, little is known about their roles in maize kernel development. Using publically available gene expression profiling data, we found that two 14-3-3 species genes, zmgf14-4 and zmgf14-6, exhibited prominent expression profiles over other 14-3-3 protein genes during maize kernel development. More than 5000 transcripts of these two genes were identified accounting for about 1/10 of the total transcripts of genes correlating to maize kernel development. We constructed a proteomics pipeline based on the affinity chromatography, in combination with 2-DE and LC-MS/MS technologies to identify the specific client proteins of the two proteins for their functional characterization. Consequently, we identified 77 specific client proteins from the developing kernels of the inbred maize B73. More than 60% of the client proteins were commonly affinity-identified by the two 14-3-3 species and are predicted to be implicated in the fundamental functions of metabolism, protein destination and storage. In addition, we found ZmGF14-4 specifically bound to the disease- or defense-relating proteins, whilst ZmGF14-6 tended to interact with the proteins involving metabolism and cell structure. Our findings provide primary insights into the functional roles of 14-3-3 proteins in maize kernel development.
  
  
  CONCLUSIONS
  Maize kernel development is a complicated physiological process for its importance in both genetics and cereal breeding. 14-3-3 proteins form a multi-gene family participating in regulations of developmental processes in plants. However, the correlation between this protein family and maize kernel development has hardly been studied. We have for the first time found 12 14-3-3 protein genes from maize genome and studied in silico the gene transcription profiling of these genes. Comparative studies revealed that maize kernel development aroused a great number of gene expression, among which 14-3-3 protein genes took a significant proportion. We applied affinity chromatographic approach, in combination with 2-DE and LC-MS/MS, to explore the specific client proteins of two crucial 14-3-3 protein species that exhibit prominent gene expression over other members in the family during the kernel development. Assessments of the identified client proteins resulted in important information toward understanding the functional mechanism of 14-3-3 protein family in maize kernel development.
authors.affiliation: Key Laboratory for Molecular Enzymology and Engineering of the Ministry of Education, Jilin University, Changchun, 130012, P. R. China.
authors.forename: Yao
authors.initials: Y
authors.lastname: Dou
authors.affiliation: Institute of Agricultural Biotechnology, Jilin Academy of Agricultural Sciences (JAAS), Changchun, 130124, P. R. China.
authors.forename: Xiangguo
authors.initials: X
authors.lastname: Liu
authors.affiliation: Institute of Agricultural Biotechnology, Jilin Academy of Agricultural Sciences (JAAS), Changchun, 130124, P. R. China.
authors.forename: Yuejia
authors.initials: Y
authors.lastname: Yin
authors.affiliation: Institute of Agricultural Biotechnology, Jilin Academy of Agricultural Sciences (JAAS), Changchun, 130124, P. R. China.
authors.forename: Siping
authors.initials: S
authors.lastname: Han
authors.affiliation: Institute of Plant Protection, Jilin Academy of Agricultural Sciences (JAAS), Changchun, 130124, P. R. China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Lu
authors.affiliation: Institute of Agricultural Biotechnology, Jilin Academy of Agricultural Sciences (JAAS), Changchun, 130124, P. R. China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Institute of Agricultural Biotechnology, Jilin Academy of Agricultural Sciences (JAAS), Changchun, 130124, P. R. China; Key Laboratory for Molecular Enzymology and Engineering of the Ministry of Education, Jilin University, Changchun, 130012, P. R. China. Electronic address: dyhao@cjaas.com.
authors.forename: Dongyun
authors.initials: D
authors.lastname: Hao
mesh_terms: D048948:14-3-3 Proteins / Q000032:analysis / Q000378:metabolism / Q000502:physiology*; D002846:Chromatography, Affinity; D015180:Electrophoresis, Gel, Two-Dimensional; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D010940:Plant Proteins / Q000032:analysis / Q000378:metabolism / Q000502:physiology*; D053719:Tandem Mass Spectrometry; D003313:Zea mays / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism*
chemical_list: D048948:14-3-3 Proteins; D010940:Plant Proteins
keywords: 14-3-3 protein species; Affinity chromatography; Functional characterization; Kernel development; Zea mays