title: Sweetpotato vines hydrolysate promotes single cell oils production of Trichosporon fermentans in high-density molasses fermentation.
abstract: This study investigated the co-fermentation of molasses and sweetpotato vine hydrolysate (SVH) by Trichosporon fermentans. T. fermentans showed low lipid accumulation on pure molasses; however, its lipid content increased by 35% when 10% SVH was added. The strong influence of SVH on lipid production was further demonstrated by the result of sensitivity analysis on effects of factors based on an artificial neural network model because the relative importance value of SVH dosage for lipid production was only lower than that of fermentation time. Scanning electron microscope observation and flow cytometry of yeast cells grown in culture with and without SVH showed that less deformation cells were involved in the culture with SVH. The activity of malic enzyme, which plays a key role in fatty acid synthesis, increased from 2.4U/mg to 3.7U/mg after SVH added. All results indicated SVH is a good supplement for lipid fermentation on molasses.
authors.affiliation: Institute of Microbiology, College of Life Sciences, Zhejiang University, Hangzhou 310058, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Shen
authors.affiliation: Institute of Environment Resource and Soil Fertilizer, Zhejiang Academy of Agriculture Science, Hangzhou 310021, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Lin
authors.affiliation: Institute of Microbiology, College of Life Sciences, Zhejiang University, Hangzhou 310058, China.
authors.forename: Qun
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Institute of Microbiology, College of Life Sciences, Zhejiang University, Hangzhou 310058, China.
authors.forename: Xiaoping
authors.initials: X
authors.lastname: Fan
authors.affiliation: Key Laboratory of Aquatic Botany and Watershed Ecology, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan 430074, China.
authors.forename: Yuyi
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Institute of Microbiology, College of Life Sciences, Zhejiang University, Hangzhou 310058, China. Electronic address: yhzhao225@zju.edu.cn.
authors.forename: Yuhua
authors.initials: Y
authors.lastname: Zhao
mesh_terms: D019149:Bioreactors; D005285:Fermentation; D005434:Flow Cytometry; D027723:Ipomoea batatas / Q000737:chemistry*; D050356:Lipid Metabolism; D008855:Microscopy, Electron, Scanning; D008965:Molasses; D016571:Neural Networks, Computer; D014250:Trichosporon / Q000378:metabolism* / Q000648:ultrastructure
keywords: Artificial neural network; Molasses; Single cell oils (SCOs); Sweetpotato vines hydrolysate; T. fermentans