title: Editing plant genomes with CRISPR/Cas9.
abstract: CRISPR/Cas9 is a rapidly developing genome editing technology that has been successfully applied in many organisms, including model and crop plants. Cas9, an RNA-guided DNA endonuclease, can be targeted to specific genomic sequences by engineering a separately encoded guide RNA with which it forms a complex. As only a short RNA sequence must be synthesized to confer recognition of a new target, CRISPR/Cas9 is a relatively cheap and easy to implement technology that has proven to be extremely versatile. Remarkably, in some plant species, homozygous knockout mutants can be produced in a single generation. Together with other sequence-specific nucleases, CRISPR/Cas9 is a game-changing technology that is poised to revolutionise basic research and plant breeding.
authors.affiliation: The Sainsbury Laboratory, Norwich Research Park, Norwich NR4 7UH, UK.
authors.forename: Khaoula
authors.initials: K
authors.lastname: Belhaj
authors.affiliation: The Sainsbury Laboratory, Norwich Research Park, Norwich NR4 7UH, UK.
authors.forename: Angela
authors.initials: A
authors.lastname: Chaparro-Garcia
authors.affiliation: The Sainsbury Laboratory, Norwich Research Park, Norwich NR4 7UH, UK. Electronic address: sophien.kamoun@tsl.ac.uk.
authors.forename: Sophien
authors.initials: S
authors.lastname: Kamoun
authors.affiliation: The Sainsbury Laboratory, Norwich Research Park, Norwich NR4 7UH, UK. Electronic address: nicola.patron@tsl.ac.uk.
authors.forename: Nicola J
authors.initials: NJ
authors.lastname: Patron
authors.affiliation: The Sainsbury Laboratory, Norwich Research Park, Norwich NR4 7UH, UK. Electronic address: vladimir.nekrasov@tsl.ac.uk.
authors.forename: Vladimir
authors.initials: V
authors.lastname: Nekrasov
mesh_terms: D000818:Animals; D064130:CRISPR-Associated Proteins / Q000235:genetics / Q000378:metabolism; D064112:Clustered Regularly Interspaced Short Palindromic Repeats; D004720:Endonucleases / Q000378:metabolism; D018745:Genome, Plant; D023281:Genomics; D006801:Humans; D010944:Plants / Q000235:genetics* / Q000378:metabolism
chemical_list: D064130:CRISPR-Associated Proteins; D004720:Endonucleases