title: Digital gene expression analysis in hemocytes of the white shrimp Litopenaeus vannamei in response to low salinity stress.
abstract: The white shrimp Litopenaeus vannamei has been greatly impacted by low salinity stress. To gain knowledge on the immune response in L. vannamei under such stress, we investigated digital gene expression (DEG) in L. vannamei hemocytes using the deep-sequencing platform Illumina HiSeq 2000. In total, 38,155 high quality unigenes with average length 770 bp were generated; 145 and 79 genes were identified up- or down-regulated, respectively. Functional categorization and pathways of the differentially expressed genes revealed that immune signaling pathways, cellular immunity, humoral immunity, apoptosis, cellular protein synthesis, lipid transport and energy metabolism were the differentially regulated processes occurring during low salinity stress. These results will provide a resource for subsequent gene expression studies regarding environmental stress and a valuable gene information for a better understanding of immune mechanisms of L. vannamei under low salinity stress.
authors.affiliation: The Key Laboratory of Mariculture, Ministry of Education, Fisheries College, Ocean University of China, Yushan Road 5, Qingdao 266003, PR China.
authors.forename: Qun
authors.initials: Q
authors.lastname: Zhao
authors.affiliation: The Key Laboratory of Mariculture, Ministry of Education, Fisheries College, Ocean University of China, Yushan Road 5, Qingdao 266003, PR China. Electronic address: panlq@ouc.edu.cn.
authors.forename: Luqing
authors.initials: L
authors.lastname: Pan
authors.affiliation: The Key Laboratory of Mariculture, Ministry of Education, Fisheries College, Ocean University of China, Yushan Road 5, Qingdao 266003, PR China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Ren
authors.affiliation: The Key Laboratory of Mariculture, Ministry of Education, Fisheries College, Ocean University of China, Yushan Road 5, Qingdao 266003, PR China.
authors.forename: Dongxu
authors.initials: D
authors.lastname: Hu
mesh_terms: D000818:Animals; D060829:Arthropod Proteins / Q000235:genetics* / Q000378:metabolism; D015723:Gene Library; D006434:Hemocytes / Q000276:immunology* / Q000378:metabolism; D059014:High-Throughput Nucleotide Sequencing; D007113:Immunity, Innate; D033561:Penaeidae / Q000235:genetics / Q000276:immunology / Q000502:physiology*; D011897:Random Allocation; D054712:Salinity; D013312:Stress, Physiological; D059467:Transcriptome
chemical_list: D060829:Arthropod Proteins
keywords: Digital gene expression; Hemocyte; Litopenaeus vannamei; Low salinity stress