title: Effect of carbon sources on growth and lipid accumulation of newly isolated microalgae cultured under mixotrophic condition.
abstract: In order to produce microalgal lipids that can be transformed to biodiesel fuel, one isolate with high lipid content was identified as Chlorella sp. Y8-1. The growth and lipid productivity of an isolated microalga Chlorella sp. Y8-1 were investigated under different cultivation conditions, including autotrophic growth (CO2, with light), heterotrophic growth (sucrose, without light) and mixotrophic growth (organic carbon sources and CO2, with light). Mixotrophic Chlorella sp. Y8-1 showed higher lipid content (35.5±4.2%) and higher lipid productivity (0.01 g/L/d) than Chlorella sp. Y8-1 cultivated under autotrophic and heterotrophic conditions on modified Walne medium. Fatty acid analysis of Chlorella sp. Y8-1 showed the major presence of palmitic acid (C16:0), oleic acid (C18:1), linoleic acid (C18:2) and linolenic acids (C18:3). The main fatty acid compositions of the Chlorella sp. Y8-1 are appropriate for biodiesel production.
authors.affiliation: Department of Bioindustry Technology, Da-Yeh University, No. 168, University Rd., Dacun, Changhua 51591, Taiwan.
authors.forename: Tse-Shih
authors.initials: TS
authors.lastname: Lin
authors.affiliation: Department of Bioindustry Technology, Da-Yeh University, No. 168, University Rd., Dacun, Changhua 51591, Taiwan. Electronic address: jywu@mail.dyu.edu.tw.
authors.forename: Jane-Yii
authors.initials: JY
authors.lastname: Wu
mesh_terms: D052818:Autotrophic Processes / Q000187:drug effects; D018533:Biomass; D050260:Carbohydrate Metabolism / Q000187:drug effects; D002244:Carbon / Q000494:pharmacology*; D005227:Fatty Acids / Q000378:metabolism; D052836:Heterotrophic Processes / Q000187:drug effects; D008055:Lipids / Q000096:biosynthesis*; D058086:Microalgae / Q000187:drug effects / Q000254:growth & development* / Q000302:isolation & purification / Q000378:metabolism*; D052817:Phototrophic Processes / Q000187:drug effects; D010802:Phylogeny; D013395:Sucrose / Q000494:pharmacology; D013997:Time Factors
chemical_list: D005227:Fatty Acids; D008055:Lipids; D013395:Sucrose; D002244:Carbon
keywords: Biodiesel; Chlorella sp.; Fatty acid; Lipid; Mixotrophic