title: Molecular cloning of precursors for TEP-1 and TEP-2: The GGNG peptide-related peptides of a prosobranch gastropod, Thais clavigera.
abstract: TEP (Thais excitatory peptide)-1 and TEP-2 are molluscan counterparts of annelidan GGNG-peptides, identified in a neogastropod, Thais clavigera (Morishita et al., 2006). We have cloned two cDNAs encoding TEP-1 and TEP-2 precursor protein, respectively, by the standard molecular cloning techniques. Predicted TEP-1 precursor protein consists of 161 amino acids, while predicted TEP-2 precursor protein has 118 amino acids. Only a single copy of TEP was found on the respective precursor. The semi-quantitative RT-PCR showed that expression of TEP-1 was high in sub-esophageal, pleural, pedal and visceral ganglia, while it was low in supra-esophageal ganglion. By contrast, expression level of TEP-2 was high in pedal and visceral ganglia. In situ hybridization visualized different subsets of TEP-1 and TEP-2 expressing neurons in Thais ganglia. For example, supra-esophageal ganglion contained many TEP-2 expressing neuron, but not TEP-1 expressing ones. These results suggest that expression of TEP-1 and TEP-2 is differently regulated in the Thais ganglia.
authors.affiliation: Department of Biological Science, Graduate School of Science, Hiroshima University, 1-3-1 Kagamiyama, Higashi-Hiroshima, Hiroshima 739-8526, Japan. Electronic address: fumi425@hiroshima-u.ac.jp.
authors.forename: Fumihiro
authors.initials: F
authors.lastname: Morishita
authors.affiliation: Division of Human Sciences, Graduate School of Integrated Arts and Sciences, Hiroshima University, 1-7-1 Kagamiyama, Higashi-Hiroshima, Hiroshima 739-8521, Japan.
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Furukawa
authors.affiliation: Division of Human Sciences, Graduate School of Integrated Arts and Sciences, Hiroshima University, 1-7-1 Kagamiyama, Higashi-Hiroshima, Hiroshima 739-8521, Japan.
authors.forename: Yu
authors.initials: Y
authors.lastname: Kodani
authors.affiliation: Suntory Foundation for Life Sciences, 1-1-1 Wakayamadai, Shimamoto, Osaka 618-8503, Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Minakata
authors.affiliation: National Institute for Environmental Studies, 16-2 Onogawa, Tsukuba, Ibaraki 305-8506 Japan.
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Horiguchi
authors.affiliation: Department of Global Environment Studies, Faculty of Environmental Studies, Hiroshima Institute of Technology, 2-1-1 Miyake, Saeki-ku, Hiroshima 731-5193, Japan.
authors.forename: Osamu
authors.initials: O
authors.lastname: Matsushima
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002490:Central Nervous System / Q000166:cytology / Q000378:metabolism; D003001:Cloning, Molecular; D005260:Female; D005724:Ganglia / Q000166:cytology / Q000378:metabolism; D049851:Gastropoda / Q000235:genetics* / Q000378:metabolism; D015870:Gene Expression; D008297:Male; D008969:Molecular Sequence Data; D009479:Neuropeptides / Q000235:genetics* / Q000378:metabolism; D009928:Organ Specificity; D011498:Protein Precursors / Q000235:genetics* / Q000378:metabolism
chemical_list: D009479:Neuropeptides; D011498:Protein Precursors
keywords: In situ hybridization; Mollusk; Neuropeptide; Precursor; RT-PCR; cDNA-library