title: Monotropein exerts protective effects against IL-1β-induced apoptosis and catabolic responses on osteoarthritis chondrocytes.
abstract: Osteoarthritis, characterized by a loss of articular cartilage accompanied with inflammation, is the most common age-associated degenerative disease. Monotropein, an iridoids glycoside isolated from the roots of Morinda officinalis How, has been demonstrated to exhibit anti-inflammatory activity. In the present study, monotropein was firstly to exhibit cartilage protective activity by down regulating the pro-inflammatory cytokines in the knee synovial fluid in vivo. The anti-apoptotic and anti-catabolic effects of monotropein on rat OA chondrocytes treated by IL-1β were investigated in vitro. In cultured chondrocytes, monotropein attenuated apoptosis in a dose-dependent manner in response to IL-1β stimulation. Moreover, treatment with monotropein, the expressions of MMP-3 and MMP-13 were significantly decreased, the expression of COL2A1 was increased. Taken together, these findings suggested that monotropein exerted anti-apoptosis and anti-catabolic activity in chondrocytes, which might support its possible therapeutic role in OA.
authors.forename: Feng
authors.initials: F
authors.lastname: Wang
authors.forename: Longhuo
authors.initials: L
authors.lastname: Wu
authors.forename: Linfu
authors.initials: L
authors.lastname: Li
authors.forename: Siyi
authors.initials: S
authors.lastname: Chen
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000302:isolation & purification / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D015153:Blotting, Western; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D019902:Chondrocytes / Q000187:drug effects* / Q000276:immunology / Q000473:pathology; D004195:Disease Models, Animal; D053583:Interleukin-1beta / Q000235:genetics / Q000276:immunology*; D039823:Iridoids / Q000302:isolation & purification / Q000494:pharmacology*; D053509:Matrix Metalloproteinase 13 / Q000235:genetics; D019278:Matrix Metalloproteinase 3 / Q000235:genetics; D032066:Morinda / Q000737:chemistry; D010003:Osteoarthritis / Q000276:immunology / Q000473:pathology; D018517:Plant Roots / Q000737:chemistry; D061251:Primary Cell Culture; D011919:Rats, Inbred Strains
chemical_list: D000893:Anti-Inflammatory Agents; D053583:Interleukin-1beta; D039823:Iridoids; C507582:monotropein; D053509:Matrix Metalloproteinase 13; D019278:Matrix Metalloproteinase 3