title: A role for human mitochondrial complex II in the production of reactive oxygen species in human skin.
abstract: The mitochondrial respiratory chain is a major generator of cellular oxidative stress, thought to be an underlying cause of the carcinogenic and ageing process in many tissues including skin. Previous studies of the relative contributions of the respiratory chain (RC) complexes I, II and III towards production of reactive oxygen species (ROS) have focussed on rat tissues and certainly not on human skin which is surprising as this tissue is regularly exposed to UVA in sunlight, a potent generator of cellular oxidative stress. In a novel approach we have used an array of established specific metabolic inhibitors and DHR123 fluorescence to study the relative roles of the mitochondrial RC complexes in cellular ROS production in 2 types of human skin cells. These include additional enhancement of ROS production by exposure to physiological levels of UVA. The effects within epidermal and dermal derived skin cells are compared to other tissue cell types as well as those harbouring a compromised mitochondrial status (Rho-zero A549). The results show that the complex II inhibitor, TTFA, was the only RC inhibitor to significantly increase UVA-induced ROS production in both skin cell types (P<0.05) suggesting that the role of human skin complex II in terms of influencing ROS production is more important than previously thought particularly in comparison to liver cells. Interestingly, two-fold greater maximal activity of complex II enzyme was observed in both skin cell types compared to liver (P<0.001). The activities of RC enzymes appear to decrease with increasing age and telomere length is correlated with ageing. Our study showed that the level of maximal complex II activity was higher in the MRC5/hTERT (human lung fibroblasts transfected with telomerase) cells than the corresponding wild type cells (P=0.0012) which can be considered (in terms of telomerase activity) as models of younger and older cells respectively.
authors.affiliation: Dermatological Sciences, Institute of Cellular Medicine, Newcastle University, Newcastle upon Tyne NE2 4HH, UK.
authors.forename: Alasdair
authors.initials: A
authors.lastname: Anderson
authors.affiliation: Dermatological Sciences, Institute of Cellular Medicine, Newcastle University, Newcastle upon Tyne NE2 4HH, UK.
authors.forename: Amy
authors.initials: A
authors.lastname: Bowman
authors.affiliation: Dermatological Sciences, Institute of Cellular Medicine, Newcastle University, Newcastle upon Tyne NE2 4HH, UK.
authors.forename: Sarah Jayne
authors.initials: SJ
authors.lastname: Boulton
authors.affiliation: Diagnostic and Therapeutic Technologies, Institute of Cellular Medicine, Newcastle University, Newcastle upon Tyne NE2 4HH, UK.
authors.forename: Philip
authors.initials: P
authors.lastname: Manning
authors.affiliation: Dermatological Sciences, Institute of Cellular Medicine, Newcastle University, Newcastle upon Tyne NE2 4HH, UK. Electronic address: m.a.birch-machin@newcastle.ac.uk.
authors.forename: Mark A
authors.initials: MA
authors.lastname: Birch-Machin
mesh_terms: D002460:Cell Line; D042963:Electron Transport Complex II / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D008928:Mitochondria / Q000201:enzymology* / Q000473:pathology; D024101:Mitochondrial Proteins / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000378:metabolism*; D012867:Skin / Q000201:enzymology* / Q000473:pathology; D014466:Ultraviolet Rays / Q000009:adverse effects*
chemical_list: D024101:Mitochondrial Proteins; D017382:Reactive Oxygen Species; D042963:Electron Transport Complex II
keywords: Ageing; Mitochondria; Reactive oxygen species (ROS); Respiratory chain; Skin