title: Development of a sensitive and specific multiplex PCR method for the simultaneous detection of chicken, duck and goose DNA in meat products.
abstract: Identifying the origin of animal species in manufactured meat products is of considerable economic, religious and sanitary importance. In this study, we developed a multiplex PCR method to simultaneously detect chicken, duck and goose DNA in meat products derived from beef, pork, mutton or quail. The PCR primers were designed based on the sequence of mitochondrial genes of each avian species, and the amplicon sizes were 131, 283 and 387bp for chicken, duck and goose, respectively. The method had no cross-reaction with DNA isolated from beef, mutton, pork or quail, and generated products at a target DNA content as low as 0.05ng, or a target meat content of 1% of total meat weight. Moreover, screening of 24 commercial meat samples using this method indicated that six, two and one samples were contaminated with chicken, duck, or both, respectively, suggesting its usefulness for the simultaneous identification of chicken, duck and goose DNA in commercial meat products.
authors.affiliation: National Key Lab of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, 430070 Wuhan, PR China. Electronic address: houbohouwei@126.com.
authors.forename: Bo
authors.initials: B
authors.lastname: Hou
authors.affiliation: National Key Lab of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, 430070 Wuhan, PR China. Electronic address: xianrongmeng@mail.hzau.edu.cn.
authors.forename: Xianrong
authors.initials: X
authors.lastname: Meng
authors.affiliation: National Key Lab of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, 430070 Wuhan, PR China.
authors.forename: Liyuan
authors.initials: L
authors.lastname: Zhang
authors.affiliation: National Key Lab of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, 430070 Wuhan, PR China.
authors.forename: Jinyue
authors.initials: J
authors.lastname: Guo
authors.affiliation: National Key Lab of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, 430070 Wuhan, PR China.
authors.forename: Shaowen
authors.initials: S
authors.lastname: Li
authors.affiliation: National Key Lab of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, 430070 Wuhan, PR China.
authors.forename: Hui
authors.initials: H
authors.lastname: Jin
mesh_terms: D000818:Animals; D002417:Cattle; D002645:Chickens / Q000235:genetics*; D003132:Commerce; D004247:DNA / Q000032:analysis*; D017931:DNA Primers; D004372:Ducks / Q000235:genetics*; D005506:Food Contamination / Q000032:analysis*; D005777:Geese / Q000235:genetics*; D006801:Humans; D008460:Meat / Q000032:analysis*; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods*; D011784:Quail; D012756:Sheep; D013552:Swine
chemical_list: D017931:DNA Primers; D004247:DNA
keywords: Food authenticity; Mitochondrial DNA; Multiplex PCR; Poultry; Species identification