title: Therapeutic ultrasound suppresses neuropathic pain and upregulation of substance P and neurokinin-1 receptor in rats after peripheral nerve injury.
abstract: We studied the mechanisms and impact of therapeutic ultrasound (TU) for pain caused by nerve injury. TU began on post-operative day 5 (POD5) and then continued daily for the next 22 d. Sensitivity to thermal and mechanical stimuli and levels of neurokinin-1 receptor, substance P, tumor necrosis factor-α and interleukin-6 in the sciatic nerve were examined. On POD7, chronic constriction injury rats undergoing TU at an intensity of 1 W/cm(2), but not 0.25 or 0.5 W/cm(2), had increases in both the mechanical withdrawal threshold and the thermal withdrawal latency compared with the chronic constriction injury group. Moreover, chronic constriction injury rats exhibited upregulation of neurokinin-1 receptor, substance P, tumor necrosis factor-α and interleukin-6 in the sciatic nerve on PODs 14 and 28, whereas TU inhibited their increased expression. We suggest that the efficacy of TU is dependent on its ability to limit the upregulation of neurokinin-1 receptor, substance P, tumor necrosis factor-α and interleukin-6 around the injured sciatic nerve.
authors.affiliation: Department of Physical Therapy and Graduate Institute of Rehabilitation Science, China Medical University, Taichung, Taiwan; Department of Medical Research, Chi-Mei Medical Center, Tainan, Taiwan.
authors.forename: Yu-Wen
authors.initials: YW
authors.lastname: Chen
authors.affiliation: Department of Food Sciences and Technology, Chia Nan University of Pharmacy and Science, Jen-Te, Tainan City, Taiwan; Department of Anesthesiology, Chi-Mei Medical Center, Yong Kang, Tainan City, Taiwan.
authors.forename: Jann-Inn
authors.initials: JI
authors.lastname: Tzeng
authors.affiliation: Department of Physical Therapy, National Cheng Kung University, Tainan, Taiwan.
authors.forename: Po-Ching
authors.initials: PC
authors.lastname: Huang
authors.affiliation: Department of Physical Therapy, National Cheng Kung University, Tainan, Taiwan. Electronic address: chhung@mail.ncku.edu.tw.
authors.forename: Ching-Hsia
authors.initials: CH
authors.lastname: Hung
authors.affiliation: Department of Cosmetics Application and Management, Chung Hwa University of Medical Technology, Tainan, Taiwan.
authors.forename: Dong-Zi
authors.initials: DZ
authors.lastname: Shao
authors.affiliation: Department of Medical Research, Chi-Mei Medical Center, Tainan, Taiwan.
authors.forename: Jhi-Joung
authors.initials: JJ
authors.lastname: Wang
mesh_terms: D000818:Animals; D008297:Male; D009437:Neuralgia / Q000209:etiology / Q000378:metabolism* / Q000517:prevention & control*; D051381:Rats; D017207:Rats, Sprague-Dawley; D018040:Receptors, Neurokinin-1 / Q000378:metabolism*; D020426:Sciatic Neuropathy / Q000150:complications / Q000378:metabolism* / Q000628:therapy*; D013373:Substance P / Q000378:metabolism*; D016896:Treatment Outcome; D014464:Ultrasonic Therapy / Q000379:methods*; D015854:Up-Regulation
chemical_list: D018040:Receptors, Neurokinin-1; D013373:Substance P
keywords: Interleukin-6; Neurokinin-1 receptor; Neuropathic pain; Substance P; Therapeutic ultrasound; Tumor necrosis factor α