title: Proteome-based bacterial identification using matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS): A revolutionary shift in clinical diagnostic microbiology.
abstract: Rapid and accurate identification of microorganisms, a prerequisite for appropriate patient care and infection control, is a critical function of any clinical microbiology laboratory. Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) is a quick and reliable method for identification of microorganisms, including bacteria, yeast, molds, and mycobacteria. Indeed, there has been a revolutionary shift in clinical diagnostic microbiology. In the present review, the state of the art and advantages of MALDI-TOF MS-based bacterial identification are described. The potential of this innovative technology for use in strain typing and detection of antibiotic resistance is also discussed. This article is part of a Special Issue entitled: Medical Proteomics.
authors.affiliation: Department of Molecular Diagnosis, Graduate School of Medicine, Chiba University, Divisions of Laboratory Medicine, Clinical Genetics and Proteomics, Chiba University Hospital, 1-8-1 Inohana, Chiba City, Chiba 260-8670, Japan. Electronic address: fnomura@faculty.chiba-u.jp.
authors.forename: Fumio
authors.initials: F
authors.lastname: Nomura
mesh_terms: D001419:Bacteria / Q000145:classification* / Q000378:metabolism; D001424:Bacterial Infections / Q000175:diagnosis* / Q000382:microbiology*; D015373:Bacterial Typing Techniques / Q000379:methods*; D006801:Humans; D040901:Proteomics / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
keywords: Clinical microbiology; MALDI-TOF MS; Proteome