title: Vinculin negatively regulates transcription of MT1-MMP through MEK/ERK pathway.
abstract: Vinculin regulates a variety of cellular functions partly through stabilization of tumor suppressor PTEN. In order to study the role of vinculin in tumor progression other than PTEN stabilization, vinculin was knocked down in PTEN-deficient squamous cell carcinoma HSC-4 cells. Knockdown of vinculin induced phenotypical change by reducing cell-cell and cell-extracellular matrix adhesions, and enhanced MT1-MMP expression at transcription level and subsequent cell migration. Up-regulation of MT1-MMP transcription by vinculin knockdown was abrogated by ERK inhibition. These results suggest that vinculin negatively regulates malignant phenotype of tumor cells including MT1-MMP transcription through MEK/ERK pathway.
authors.affiliation: Division of Molecular Virology and Oncology, Cancer Research Institute, Kanazawa University, Kakuma-machi, Kanazawa 920-1192, Japan; Department of Oral and Maxillofacial Surgery, Graduate School of Medical Science, Kanazawa University, 13-1 Takara-machi, Kanazawa 920-8641, Japan.
authors.forename: Taisuke
authors.initials: T
authors.lastname: Yoshimoto
authors.affiliation: Division of Molecular Virology and Oncology, Cancer Research Institute, Kanazawa University, Kakuma-machi, Kanazawa 920-1192, Japan. Electronic address: ttakino@staff.kanazawa-u.ac.jp.
authors.forename: Takahisa
authors.initials: T
authors.lastname: Takino
authors.affiliation: Division of Molecular Virology and Oncology, Cancer Research Institute, Kanazawa University, Kakuma-machi, Kanazawa 920-1192, Japan.
authors.forename: Zichen
authors.initials: Z
authors.lastname: Li
authors.affiliation: Division of Molecular Virology and Oncology, Cancer Research Institute, Kanazawa University, Kakuma-machi, Kanazawa 920-1192, Japan.
authors.forename: Takahiro
authors.initials: T
authors.lastname: Domoto
authors.affiliation: Division of Molecular Virology and Oncology, Cancer Research Institute, Kanazawa University, Kakuma-machi, Kanazawa 920-1192, Japan. Electronic address: vhsato@staff.kanazawa-u.ac.jp.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Sato
mesh_terms: D002450:Cell Communication; D045744:Cell Line, Tumor; D002465:Cell Movement; D003094:Collagen / Q000378:metabolism; D004789:Enzyme Activation; D005109:Extracellular Matrix / Q000378:metabolism; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic; D006801:Humans; D020935:MAP Kinase Signaling System; D053511:Matrix Metalloproteinase 14 / Q000378:metabolism*; D010641:Phenotype; D011379:Prognosis; D034741:RNA, Small Interfering / Q000378:metabolism; D014158:Transcription, Genetic; D016596:Vinculin / Q000378:metabolism*
chemical_list: D034741:RNA, Small Interfering; C488955:VCL protein, human; D016596:Vinculin; D003094:Collagen; D048049:Extracellular Signal-Regulated MAP Kinases; C507086:MMP14 protein, human; D053511:Matrix Metalloproteinase 14
keywords: Cancer; Cell–extracellular matrix adhesion; MT1-MMP; Migration; Vinculin