title: Arthrographis kalrae soluble antigens present hemolytic and cytotoxic activities.
abstract: Arthrographis kalrae is a dimorphic, cosmopolitan and neurotropic fungus that has been described as a rare human pathogen. This study investigated the hemolytic and cytotoxic activities of A. kalrae cell-free antigens (CFAs). Total CFAs and their Sephadex chromatography fractions were tested on mouse erythrocytes for hemolysis and on the P3U1 cell line for cytotoxicity. Hemolytic and cytotoxic activities were detected in distinct molecular mass (MM) fractions. Additionally, antibodies against isogenic erythrocytes sensitized with CFAs (anti-E-CFAs) inhibited hemolysis but not cytotoxicity. Hemolysis was not affected by heating, and a higher reactivity was detected in the carbohydrate-rich fractions, which decreased after reduction by periodate treatment. The pioneering nature of this work is due to the demonstration of the cytotoxic activity in A. kalrae and the suggestion that this activity may be due to molecules distinct from the hemolytic factor, with the latter potentially being a component with a high MM.
authors.affiliation: State University of Londrina, Rod. Celso Garcia Cid, km 380, 86057-970 Londrina, PR, Brazil.
authors.forename: Luciene Airy
authors.initials: LA
authors.lastname: Nagashima
authors.affiliation: State University of Londrina, Rod. Celso Garcia Cid, km 380, 86057-970 Londrina, PR, Brazil.
authors.forename: Claudia Yuri
authors.initials: CY
authors.lastname: Akagi
authors.affiliation: University of the Ryukyus, Okinawa, Japan.
authors.forename: Ayako
authors.initials: A
authors.lastname: Sano
authors.affiliation: State University of Londrina, Rod. Celso Garcia Cid, km 380, 86057-970 Londrina, PR, Brazil.
authors.forename: Paula Leonello
authors.initials: PL
authors.lastname: Álvares e Silva
authors.affiliation: Murata Animal Hospital, Mobara, Chiba, Japan.
authors.forename: Yoshiteru
authors.initials: Y
authors.lastname: Murata
authors.affiliation: State University of Londrina, Rod. Celso Garcia Cid, km 380, 86057-970 Londrina, PR, Brazil. Electronic address: itanoeiko@hotmail.com.
authors.forename: Eiko Nakagawa
authors.initials: EN
authors.lastname: Itano
mesh_terms: D000818:Animals; D000946:Antigens, Fungal / Q000302:isolation & purification / Q000494:pharmacology*; D001402:B-Lymphocytes / Q000166:cytology / Q000187:drug effects*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D002850:Chromatography, Gel; D004912:Erythrocytes / Q000166:cytology / Q000187:drug effects*; D006461:Hemolysis / Q000187:drug effects*; D006358:Hot Temperature; D051379:Mice; D008970:Molecular Weight; D010504:Periodic Acid / Q000737:chemistry; D004718:Saccharomycetales / Q000737:chemistry*; D012995:Solubility
chemical_list: D000946:Antigens, Fungal; D010504:Periodic Acid; C009288:metaperiodate
keywords: Antibodies; Chromatography; Cytotoxicity; Fungus; Hemolysis