title: Analysis of microvasculature phenotype and endothelial activation markers in skin lesions of lacaziosis (Lobomycosis).
abstract: Jorge Lobo's disease is a rare mycosis characterized by chronic inflammation, which causes skin lesions in the absence of visceral dissemination. The disease occurs mainly in hot and humid climates and most cases have been registered in the Brazilian Amazon region. This study investigated possible microvascular alterations in skin lesions caused by infection with Lacazia loboi which may interfere with the clinical progression of the disease. Immunohistochemistry was used to evaluate the density of blood and lymphatic vessels, as well as expression of the cell adhesion molecules ICAM-1, VCAM-1 and E-selectin. The results showed a reduced number of blood (62.66 ± 20.30 vessels/mm(2)) and lymphatic vessels (3.55 ± 5.84 vessels/mm(2)) in Jorge Lobo's disease when compared to control skin (169.66 ± 66.38 blood vessels/mm(2) and 8 ± 2.17 lymphatic vessels/mm(2)). There were a larger number of vessels expressing ICAM-1 (27.58 ± 15.32 vessels/mm(2)) and VCAM-1 (7.55 ± 6.2 vessels/mm(2)). No difference was observed in the expression of E-selectin (4.66 ± 11 vessels/mm(2)). Taken together, the results indicate changes in the local microvasculature which may interfere with the development of an efficient cell-mediated immune response and may explain restriction of the fungus to the site of injury.
authors.affiliation: Nucleo de Medicina Tropical, Universidade Federal do Para, Belem, PA, Brazil; Centro de Ciencias Biologicas e da Saude, Universidade do Estado do Para, Belem, PA, Brazil. Electronic address: juarez@pesquisador.cnpq.br.
authors.forename: Juarez A S
authors.initials: JA
authors.lastname: Quaresma
authors.affiliation: Nucleo de Medicina Tropical, Universidade Federal do Para, Belem, PA, Brazil.
authors.forename: Maysa V
authors.initials: MV
authors.lastname: Brito
authors.affiliation: Nucleo de Medicina Tropical, Universidade Federal do Para, Belem, PA, Brazil.
authors.forename: Jorge R
authors.initials: JR
authors.lastname: Sousa
authors.affiliation: Centro de Ciencias Biologicas e da Saude, Universidade do Estado do Para, Belem, PA, Brazil.
authors.forename: Luciana M
authors.initials: LM
authors.lastname: Silva
authors.affiliation: Centro de Ciencias Biologicas e da Saude, Universidade do Estado do Para, Belem, PA, Brazil.
authors.forename: Kelly E
authors.initials: KE
authors.lastname: Hirai
authors.affiliation: Centro de Ciencias Biologicas e da Saude, Universidade do Estado do Para, Belem, PA, Brazil.
authors.forename: Rafael S
authors.initials: RS
authors.lastname: Araujo
authors.affiliation: Nucleo de Medicina Tropical, Universidade Federal do Para, Belem, PA, Brazil.
authors.forename: Arival C
authors.initials: AC
authors.lastname: de Brito
authors.affiliation: Centro de Ciencias Biologicas e da Saude, Universidade do Estado do Para, Belem, PA, Brazil.
authors.forename: Francisca R O
authors.initials: FR
authors.lastname: Carneiro
authors.affiliation: Nucleo de Medicina Tropical, Universidade Federal do Para, Belem, PA, Brazil.
authors.forename: Hellen T
authors.initials: HT
authors.lastname: Fuzii
authors.affiliation: Departamento de Patologia, Faculdade de Medicina, Universidade de Sao Paulo, Sao Paulo, SP, Brazil.
authors.forename: Carla
authors.initials: C
authors.lastname: Pagliari
authors.affiliation: Departamento de Patologia, Faculdade de Medicina, Universidade de Sao Paulo, Sao Paulo, SP, Brazil.
authors.forename: Mirian N
authors.initials: MN
authors.lastname: Sotto
authors.affiliation: Departamento de Patologia, Faculdade de Medicina, Universidade de Sao Paulo, Sao Paulo, SP, Brazil.
authors.forename: Maria I S
authors.initials: MI
authors.lastname: Duarte
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015415:Biomarkers / Q000378:metabolism; D001938:Brazil; D019040:E-Selectin / Q000235:genetics / Q000378:metabolism; D042783:Endothelial Cells / Q000378:metabolism / Q000473:pathology*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D018799:Intercellular Adhesion Molecule-1 / Q000235:genetics / Q000378:metabolism; D060367:Lacazia / Q000502:physiology*; D060368:Lobomycosis / Q000235:genetics / Q000378:metabolism / Q000382:microbiology / Q000473:pathology*; D008297:Male; D055806:Microvessels / Q000378:metabolism / Q000382:microbiology / Q000473:pathology*; D008875:Middle Aged; D012867:Skin / Q000098:blood supply* / Q000378:metabolism / Q000473:pathology; D019010:Vascular Cell Adhesion Molecule-1 / Q000235:genetics / Q000378:metabolism; D055815:Young Adult
chemical_list: D015415:Biomarkers; D019040:E-Selectin; D019010:Vascular Cell Adhesion Molecule-1; D018799:Intercellular Adhesion Molecule-1
keywords: E-selectin; ICAM-1; Jorge Lobo's disease; Microvasculature; VCAM-1