title: Study on the extraction process of total anthraquinones in Radix et Rhizoma Rhei and their antilipemic effects.
abstract: BACKGROUND
  Radix et Rhizoma Rhei has a gastric mucosal protective effect, major anti-gastritis and anti-peptic ulcer active constituents were emodin, aloe-emodin, chrysophanol, rhein, etc. The objective of the study was the extraction process of total anthraquinones in Radix et Rhizoma rhei and their antilipemic effects.
  
  
  METHODS
  Orthogonal experiment, UV spectrophotometry and conventional antilipemic effect determination method were used to optimize the extraction process, and to determine the total anthraquinone content, as well as blood levels of total cholesterol, triglycerides, HDL and LDL.
  
  
  RESULTS
  Ethanol concentration, extraction time and ethanol amount had significant influences on the extraction of total Radix et Rhizoma rhei anthraquinones, total Radix et Rhizoma rhei anthraquinones could significantly reduce blood levels of total cholesterol, triglycerides, HDL and LDL.
  
  
  CONCLUSIONS
  The optimum extraction process was two times extraction of Radix et Rhizoma rhei with 70% ethanol, the amounts of solvent of 8 folds and 5 folds, successively, and the extraction time of 60 min each. In addition, this extract has an antilipemic effect in mice.
authors.affiliation: The key laboratory of education ministry on environments and resources in Tibetan Plateau, Qinghai-Normal University, Xining 810008, China.
authors.forename: Hui-Chun
authors.initials: HC
authors.lastname: Xie
authors.affiliation: The key laboratory of education ministry on environments and resources in Tibetan Plateau, Qinghai-Normal University, Xining 810008, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Shang
mesh_terms: D000818:Animals; D000880:Anthraquinones / Q000008:administration & dosage / Q000032:analysis / Q000302:isolation & purification*; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000032:analysis / Q000302:isolation & purification*; D005260:Female; D006801:Humans; D006949:Hyperlipidemias / Q000188:drug therapy* / Q000378:metabolism; D000960:Hypolipidemic Agents / Q000008:administration & dosage / Q000032:analysis / Q000302:isolation & purification*; D008297:Male; D051379:Mice; D012250:Rheum / Q000737:chemistry*; D027343:Rhizome / Q000737:chemistry*
chemical_list: D000880:Anthraquinones; D004365:Drugs, Chinese Herbal; D000960:Hypolipidemic Agents
keywords: Radix et Rhizoma Rhei; anti-lipids; orthogonal test; total anthraquinones