title: Mycoplasma ovipneumoniae induces inflammatory response in sheep airway epithelial cells via a MyD88-dependent TLR signaling pathway.
abstract: Mycoplasma ovipneumoniae (M. ovipneumoniae) is a bacterium that specifically infects sheep and goat and causes ovine infectious pleuropneumonia. In an effort to understand the pathogen-host interaction between the M. ovipneumoniae and airway epithelial cells, we investigated the host inflammatory response using a primary air-liquid interface (ALI) epithelial culture model generated from bronchial epithelial cells of Ningxia Tan sheep (Ovis aries). The ALI culture of sheep bronchial epithelial cells showed a fully differentiated epithelium comprising distinct epithelial types, including the basal, ciliated and goblet cells. Exposure of ALI cultures to M. ovipneumoniae led to increased expression of Toll-like receptors (TLRs), and components of the myeloid differentiation factor 88 (MyD88)-dependent TLR signaling pathway, including the MyD88, TNF receptor-associated factor 6 (TRAF6), IL-1 receptor-associated kinases (IRAKs) and nuclear factor-kappa B (NF-κB), as well as subsequent pro-inflammatory cytokines in the epithelial cells. Of interest, infection with M. ovipneumoniae failed to induce the expression of TANK-binding kinase 1 (TBK1), TRAF3 and interferon regulatory factor 3 (IRF3), key components of the MyD88-independent signaling pathway. These results suggest that the MyD88-dependent TLR pathway may play a crucial role in sheep airway epithelial cells in response to M. ovipneumoniae infection, which also indicate that the ALI culture system may be a reliable model for investigating pathogen-host interactions between M. ovipneumoniae and airway epithelial cells.
authors.affiliation: Key Laboratory of Ministry of Education for Conservation and Utilization of Special Biological Resources in the Western China, China; College of Life Science, Ningxia University, Yinchuan, Ningxia 750021, China.
authors.forename: Di
authors.initials: D
authors.lastname: Xue
authors.affiliation: Key Laboratory of Ministry of Education for Conservation and Utilization of Special Biological Resources in the Western China, China; College of Life Science, Ningxia University, Yinchuan, Ningxia 750021, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Ma
authors.affiliation: Key Laboratory of Ministry of Education for Conservation and Utilization of Special Biological Resources in the Western China, China; College of Life Science, Ningxia University, Yinchuan, Ningxia 750021, China.
authors.forename: Min
authors.initials: M
authors.lastname: Li
authors.affiliation: Key Laboratory of Ministry of Education for Conservation and Utilization of Special Biological Resources in the Western China, China; College of Life Science, Ningxia University, Yinchuan, Ningxia 750021, China.
authors.forename: Yanan
authors.initials: Y
authors.lastname: Li
authors.affiliation: Key Laboratory of Ministry of Education for Conservation and Utilization of Special Biological Resources in the Western China, China; College of Life Science, Ningxia University, Yinchuan, Ningxia 750021, China.
authors.forename: Haixia
authors.initials: H
authors.lastname: Luo
authors.affiliation: Key Laboratory of Ministry of Education for Conservation and Utilization of Special Biological Resources in the Western China, China; College of Life Science, Ningxia University, Yinchuan, Ningxia 750021, China.
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Liu
authors.affiliation: Key Laboratory of Ministry of Education for Conservation and Utilization of Special Biological Resources in the Western China, China; College of Life Science, Ningxia University, Yinchuan, Ningxia 750021, China. Electronic address: wyj@nxu.edu.cn.
authors.forename: Yujiong
authors.initials: Y
authors.lastname: Wang
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D005455:Fluorescent Antibody Technique / Q000662:veterinary; D007249:Inflammation / Q000276:immunology / Q000503:physiopathology / Q000662:veterinary; D008855:Microscopy, Electron, Scanning / Q000662:veterinary; D045802:Mycoplasma ovipneumoniae / Q000276:immunology; D053594:Myeloid Differentiation Factor 88 / Q000502:physiology*; D011019:Pneumonia, Mycoplasma / Q000276:immunology / Q000382:microbiology / Q000662:veterinary*; D060888:Real-Time Polymerase Chain Reaction / Q000662:veterinary; D020545:Respiratory Mucosa / Q000276:immunology / Q000503:physiopathology*; D012756:Sheep / Q000276:immunology / Q000382:microbiology; D012757:Sheep Diseases / Q000276:immunology / Q000382:microbiology*; D015398:Signal Transduction / Q000276:immunology / Q000502:physiology*
chemical_list: D053594:Myeloid Differentiation Factor 88
keywords: Airway epithelial cells; Air–liquid interface culture; M. ovipneumoniae; Sheep; Toll-like receptor signaling