title: Nanocellulose-alginate hydrogel for cell encapsulation.
abstract: TEMPO-oxidized bacterial cellulose (TOBC)-sodium alginate (SA) composites were prepared to improve the properties of hydrogel for cell encapsulation. TOBC fibers were obtained using a TEMPO/NaBr/NaClO system at pH 10 and room temperature. The fibrillated TOBCs mixed with SA were cross-linked in the presence of Ca(2+) solution to form hydrogel composites. The compression strength and chemical stability of the TOBC/SA composites were increased compared with the SA hydrogel, which indicated that TOBC performed an important function in enhancing the structural, mechanical and chemical stability of the composites. Cells were successfully encapsulated in the TOBC/SA composites, and the viability of cells was investigated. TOBC/SA composites can be a potential candidate for cell encapsulation engineering.
authors.affiliation: Department of Biosystems and Biomaterials Science and Engineering, Seoul National University, Seoul 151-921, Republic of Korea.
authors.forename: Minsung
authors.initials: M
authors.lastname: Park
authors.affiliation: Department of Biosystems and Biomaterials Science and Engineering, Seoul National University, Seoul 151-921, Republic of Korea.
authors.forename: Dajung
authors.initials: D
authors.lastname: Lee
authors.affiliation: Department of Biosystems and Biomaterials Science and Engineering, Seoul National University, Seoul 151-921, Republic of Korea; Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul 151-921, Republic of Korea. Electronic address: jhyun@snu.ac.kr.
authors.forename: Jinho
authors.initials: J
authors.lastname: Hyun
mesh_terms: D000464:Alginates / Q000737:chemistry*; D000818:Animals; D049109:Cell Proliferation; D002470:Cell Survival; D002482:Cellulose / Q000096:biosynthesis / Q000737:chemistry*; D019245:Compressive Strength; D003497:Cyclic N-Oxides / Q000737:chemistry; D041822:Gluconacetobacter xylinus / Q000378:metabolism; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D020100:Hydrogels / Q000737:chemistry*; D051379:Mice; D008853:Microscopy / Q000379:methods; D041681:NIH 3T3 Cells; D053761:Nanocomposites / Q000737:chemistry* / Q000648:ultrastructure; D016877:Oxidants / Q000737:chemistry; D010084:Oxidation-Reduction; D011135:Polysaccharides, Bacterial / Q000096:biosynthesis / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D000464:Alginates; D003497:Cyclic N-Oxides; D006603:Hexuronic Acids; D020100:Hydrogels; D016877:Oxidants; D011135:Polysaccharides, Bacterial; D020723:Glucuronic Acid; D002482:Cellulose; C003959:TEMPO
keywords: Alginate; Bacterial cellulose; Cell encapsulation; TEMPO-oxidization