title: 13Cα CEST experiment on uniformly 13C-labeled proteins.
abstract: A new HSQC-based (13)Cα CEST pulse scheme is proposed, which is suitable for uniformly (13)C- or (13)C, (15)N-labeled samples in either water or heavy water. Except for Thr and Ser residues, the sensitivity of this scheme for uniformly labeled samples is similar to that of the previous scheme for selectively (13)Cα-labeled samples with 100% isotope enrichment. The experiment is demonstrated on an acyl carrier protein domain. Our (13)Cα CEST data reveal that the minor state of the acyl carrier protein has high helical propensity. The new scheme will facilitate structural characterization of invisible minor states.
authors.affiliation: Department of Biological Sciences, National University of Singapore, 14 Science Drive 4, Singapore, 117543, Singapore.
authors.forename: Yang
authors.initials: Y
authors.lastname: Zhou
authors.forename: Daiwen
authors.initials: D
authors.lastname: Yang
mesh_terms: D001426:Bacterial Proteins / Q000032:analysis* / Q000737:chemistry; D002247:Carbon Isotopes / Q000737:chemistry; D066241:Carbon-13 Magnetic Resonance Spectroscopy / Q000379:methods*; D008847:Micromonospora / Q000378:metabolism*; D009587:Nitrogen Isotopes / Q000737:chemistry; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D017434:Protein Structure, Tertiary
chemical_list: D001426:Bacterial Proteins; D002247:Carbon Isotopes; D009587:Nitrogen Isotopes