title: Storage of Steindachneridion parahybae oocytes at different temperatures.
abstract: The objective of this study was to assess the influence of temperature and time on the storage of fresh Steindachneridion parahybae oocytes. Two experiments were carried out: (1) the fertilization rates of oocytes exposed to temperatures of 5, 15, 28 (room temperature) and 35°C were assessed 15min (control), 115, 235 and 355min after release; (2) the fertilization and hatching rates, as well as the percentage of normal larvae of oocytes exposed to 14, 17 or 20°C, 20min (control) were assessed 50, 80 and 110min after stripping. In the first experiment, the highest fertilization rates (P<0.05) were obtained in the control treatment (15min, 28°C), with 74.34±5.48% oocytes showing loss of viability over time. In the second experiment, there was a reduction (P<0.05) in the fertilization rates at the temperatures and times tested. The artificial fertilization of S. parahybae oocytes is recommended immediately after collection, and if storage is necessary, it should be conducted at temperatures between 17 and 20°C.
authors.affiliation: State University of São Paulo, UNESP, Registro, SP, Brazil. Electronic address: sanches@registro.unesp.br.
authors.forename: Eduardo Antônio
authors.initials: EA
authors.lastname: Sanches
authors.affiliation: Fishery Institute, APTA, São Paulo, SP, Brazil.
authors.forename: Renan Yoshiharu
authors.initials: RY
authors.lastname: Okawara
authors.affiliation: São Paulo Energy Company, CESP, Paraibuna, SP, Brazil.
authors.forename: Danilo
authors.initials: D
authors.lastname: Caneppele
authors.affiliation: State University of West Paraná, UNIOESTE, Toledo, PR, Brazil.
authors.forename: Giovano
authors.initials: G
authors.lastname: Neumann
authors.affiliation: State University of West Paraná, UNIOESTE, Toledo, PR, Brazil.
authors.forename: Robie Allan
authors.initials: RA
authors.lastname: Bombardelli
authors.affiliation: Fishery Institute, APTA, São Paulo, SP, Brazil.
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Romagosa
mesh_terms: D000818:Animals; D017756:Aquaculture; D002397:Catfishes; D005260:Female; D005306:Fertilization; D008297:Male; D009865:Oocytes; D011786:Quality Control; D013696:Temperature; D014021:Tissue Preservation / Q000379:methods* / Q000592:standards
keywords: Artificial fertilization; Eggs; Fish; Gamete exposure; Larvae; Surubim-do-Paraiba