title: Binding-induced collapse of DNA nano-assembly for naked-eye detection of ATP with plasmonic gold nanoparticles.
abstract: The detection of small molecules depends heavily on complicated GC-MS (Gas chromatography-mass spectrometry), HPLC (High-performance liquid chromatography) and some other complicated instruments that are not suitable for point of care detection. Here, we have demonstrated a fast (in 10min), simple (instrument-free) and effective detection platform for small molecule-ATP. In our design, we engineered the hybridization region of aptamer and assembled it into a superstructure to avoid the exposed flexible ends. The binding of ATP triggered the collapse of the superstructures to produce single stranded DNA that can obviously tune the plasmonic coupling of unmodified gold nanoparticles (AuNPs). Compared to detection platforms based on fully hybridized aptamer double helix, the detection time was significantly decreased to 10min. The resulting color change can be recognized by naked eyes. Our detection is highly specific and selective. Furthermore, a logic gate with multiplexed detection capability for ATP and DNA were demonstrated.
authors.affiliation: School of Medical Lab Science and Life Science, Wenzhou Medical University, Wenzhou 325035, China; Division of Physical Biology & Bioimaging Center, Shanghai Synchrotron Radiation Facility, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China.
authors.forename: Jingjing
authors.initials: J
authors.lastname: Wang
authors.affiliation: School of Medical Lab Science and Life Science, Wenzhou Medical University, Wenzhou 325035, China.
authors.forename: Jianxin
authors.initials: J
authors.lastname: Lu
authors.affiliation: Key Laboratory for Organic Electronics & Information Displays (KLOEID) and Institute of Advanced Materials (IAM), Nanjing University of Posts & Telecommunications, Nanjing, China.
authors.forename: Shao
authors.initials: S
authors.lastname: Su
authors.affiliation: School of Medical Lab Science and Life Science, Wenzhou Medical University, Wenzhou 325035, China. Electronic address: jimingao@yahoo.com.
authors.forename: Jimin
authors.initials: J
authors.lastname: Gao
authors.affiliation: Division of Physical Biology & Bioimaging Center, Shanghai Synchrotron Radiation Facility, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Huang
authors.affiliation: Key Laboratory for Organic Electronics & Information Displays (KLOEID) and Institute of Advanced Materials (IAM), Nanjing University of Posts & Telecommunications, Nanjing, China.
authors.forename: Lianhui
authors.initials: L
authors.lastname: Wang
authors.affiliation: Key Laboratory for Organic Electronics & Information Displays (KLOEID) and Institute of Advanced Materials (IAM), Nanjing University of Posts & Telecommunications, Nanjing, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Huang
authors.affiliation: Division of Physical Biology & Bioimaging Center, Shanghai Synchrotron Radiation Facility, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China. Electronic address: zuoxiaolei@sinap.ac.cn.
authors.forename: Xiaolei
authors.initials: X
authors.lastname: Zuo
mesh_terms: D000255:Adenosine Triphosphate / Q000032:analysis / Q000097:blood*; D052157:Aptamers, Nucleotide / Q000737:chemistry*; D015374:Biosensing Techniques / Q000379:methods*; D003124:Colorimetry / Q000379:methods; D004277:DNA, Single-Stranded / Q000737:chemistry; D006046:Gold / Q000737:chemistry*; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry*; D009690:Nucleic Acid Conformation
chemical_list: D052157:Aptamers, Nucleotide; D004277:DNA, Single-Stranded; D006046:Gold; D000255:Adenosine Triphosphate
keywords: Biosensor; Colorimetric detection; Gold nanoparticles; Small molecules