title: Anti-inflammatory effect of chymotrypsin to autoimmune response against CNS is dose-dependent.
abstract: Multiple sclerosis is an inflammatory autoimmune disease of central nervous system (CNS) in which inflammatory cells release pro-inflammatory cytokines, proteases, and other toxic mediators. Proteases are involved in many aspects of inflammatory process. There are many reports regarding the effect of proteases on inflammation. Chymotrypsin is a serine protease with anti-inflammatory effect. We investigated chymotrypsin effect on experimental autoimmune encephalomyelitis (EAE), the animal model of multiple sclerosis. Intra-CSF injection with 0.1 mg/ml, 0.2 mg/ml chymotrypsin, or saline was done on day 7 after EAE induction. Our study demonstrated that 0.1 mg/ml chymotrypsin treatment did not decrease clinical signs, but 0.2 mg/ml chymotrypsin ameliorated clinical signs and manipulated immune response in both brain and spinal cord. Administration of 0.1 mg/ml or 0.2 mg/ml chymotrypsin led to decreased IL-17 along with increased IL-4 and FoxP3 in 0.2 mg/ml chymotrypsin-treated animals. Presumably, chymotrypsin acts in a dose-dependent manner and concentrations of chymotrypsin more than 0.2 mg/ml may have more beneficial effect.
authors.forename: Ameneh
authors.initials: A
authors.lastname: Ghaffarinia
authors.forename: Cyrus
authors.initials: C
authors.lastname: Jalili
authors.forename: Farhad
authors.initials: F
authors.lastname: Riazi-Rad
authors.forename: Ali
authors.initials: A
authors.lastname: Mostafaie
authors.forename: Shahram
authors.initials: S
authors.lastname: Parvaneh
authors.forename: Nafiseh
authors.initials: N
authors.lastname: Pakravan
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D015551:Autoimmunity / Q000187:drug effects*; D001921:Brain / Q000187:drug effects*; D002918:Chymotrypsin / Q000494:pharmacology*; D004195:Disease Models, Animal; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000188:drug therapy* / Q000276:immunology; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D011917:Rats, Inbred Lew; D013116:Spinal Cord / Q000187:drug effects*
chemical_list: D000893:Anti-Inflammatory Agents; D002918:Chymotrypsin