title: Constitutive expression and silencing of a novel seed specific calcium dependent protein kinase gene in rice reveals its role in grain filling.
abstract: Ca(2+) sensor protein kinases are prevalent in most plant species including rice. They play diverse roles in plant signaling mechanism. Thirty one CDPK genes have been identified in rice and some are functionally characterized. In the present study, the newly identified rice CDPK gene OsCPK31 was functionally validated by overexpression and silencing in Taipei 309 rice cultivar. Spikelets of overexpressing plants showed hard dough stage within 15d after pollination (DAP) with rapid grain filling and early maturation. Scanning electron microscopy of endosperm during starch granule formation confirmed early grain filling. Further, seeds of overexpressing transgenic lines matured early (20-22 DAP) and the average number of maturity days reduced significantly. On the other hand, silencing lines showed more number of unfilled spikelet without any difference in maturity duration. It will be interesting to further decipher the role of OsCPK31 in biological pathways associated with distribution of photosynthetic assimilates during grain filling stage.
authors.affiliation: Biotechnology Laboratory, Directorate of Rice Research, Rajendranagar, Hyderabad 500 030, Andhra Pradesh, India.
authors.forename: P
authors.initials: P
authors.lastname: Manimaran
authors.affiliation: Biotechnology Laboratory, Directorate of Rice Research, Rajendranagar, Hyderabad 500 030, Andhra Pradesh, India.
authors.forename: Satendra K
authors.initials: SK
authors.lastname: Mangrauthia
authors.affiliation: Biotechnology Laboratory, Directorate of Rice Research, Rajendranagar, Hyderabad 500 030, Andhra Pradesh, India.
authors.forename: R M
authors.initials: RM
authors.lastname: Sundaram
authors.affiliation: Biotechnology Laboratory, Directorate of Rice Research, Rajendranagar, Hyderabad 500 030, Andhra Pradesh, India. Electronic address: balasena@yahoo.com.
authors.forename: S M
authors.initials: SM
authors.lastname: Balachandran
mesh_terms: D015971:Gene Expression Regulation, Enzymologic; D018506:Gene Expression Regulation, Plant; D020868:Gene Silencing; D017343:Genes, Plant; D005822:Genetic Vectors / Q000378:metabolism; D009928:Organ Specificity / Q000235:genetics; D012275:Oryza / Q000201:enzymology* / Q000235:genetics*; D010641:Phenotype; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D030821:Plants, Genetically Modified; D011494:Protein Kinases / Q000235:genetics* / Q000378:metabolism; D034622:RNA Interference; D012639:Seeds / Q000201:enzymology* / Q000235:genetics; D013213:Starch / Q000648:ultrastructure; D014170:Transformation, Genetic; D017871:Calcium-Calmodulin-Dependent Protein Kinases
chemical_list: D010940:Plant Proteins; D011494:Protein Kinases; D013213:Starch; D017871:Calcium-Calmodulin-Dependent Protein Kinases
keywords: Calcium dependent protein kinase; Grain filling; OsCPK31; Rice; Seed development