title: Cell-mimetic coatings for immune spheres.
abstract: Extrinsically induced or engineered cells are providing new therapeutic means in emerging fields such as cell therapeutics, immunomodulation and regenerative medicine. We are demonstrating a spatial induction method using lipid coatings, which can change signal presentation strength from material surface to adherent macrophage cells, that induce early cell-cell interaction leading to organotypic morphology. For that, we have developed a cell mimetic lipid coating with a rafts size to the order of transmembrane proteins (<10 nm) with enhanced lateral elastic properties. Such surface coatings are capable of reducing adherent macrophage spreading, while enabling early induction of cell-cell interaction to form organotypic macrophage colonies or "spheres" (M-spheres).
authors.affiliation: Biosurface Technology Division, Biomedical Technology Wing, Sree Chitra Tirunal Institute for Medical Science and Technology, Poojappura, Thiruvananthapuram, Kerala, India. Electronic address: nairkaladhar@rediffmail.com.
authors.forename: K
authors.initials: K
authors.lastname: Kaladhar
authors.affiliation: Institute for Laboratory Medicine and Pathobiochemistry, Molecular Diagnostics, Philipps University, Marburg, Germany. Electronic address: renzh@med.uni-marburg.de.
authors.forename: H
authors.initials: H
authors.lastname: Renz
authors.affiliation: Biosurface Technology Division, Biomedical Technology Wing, Sree Chitra Tirunal Institute for Medical Science and Technology, Poojappura, Thiruvananthapuram, Kerala, India.
authors.forename: C P
authors.initials: CP
authors.lastname: Sharma
mesh_terms: D032701:Biomimetics / Q000379:methods*; D002448:Cell Adhesion / Q000502:physiology; D002450:Cell Communication / Q000502:physiology; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D006801:Humans; D008264:Macrophages / Q000166:cytology / Q000502:physiology; D013499:Surface Properties
chemical_list: D020099:Coated Materials, Biocompatible
keywords: Biomaterials; Colloids; Lipids; Macrophage; Surface coating