title: A multifactorial screening strategy to identify anti-idiotypic reagents for bioanalytical support of antibody therapeutics.
abstract: Antibodies are critical tools for protein bioanalysis; their quality and performance dictate the caliber and robustness of ligand binding assays. After immunization, polyclonal B cells generate a diverse antibody repertoire against constant and variable regions of the therapeutic antibody immunogen. Herein we describe a comprehensive and multifactorial screening strategy to eliminate undesirable constant region-specific antibodies and select for anti-idiotypic antibodies with specificity for the unique variable region. Application of this strategy is described for the therapeutic antibody Mab-A case study. Five different factors were evaluated to select a final antibody pair for the quantification of therapeutics in biological matrices: (i) matrix effect in preclinical and clinical matrices, (ii) assay sensitivity with lower limit of quantification goal of single-digit ng/ml (low pM) at a signal-to-background ratio greater than 5, (iii) epitope distinction or nonbridging antibody pair, (iv) competition with target and inhibitory capacity enabling measurement of free drug, and (v) neutralizing bioactivity using bioassay. The selected antibody pair demonstrated superior assay sensitivity with no or minimal matrix effect in common biological samples, recognized two distinct binding epitopes on the therapeutic antibody variable region, and featured inhibitory and neutralizing effects with respect to quantification of free drug levels.
authors.affiliation: Department of Pharmacokinetics and Drug Metabolism, Amgen, Thousand Oaks, CA 91320, USA. Electronic address: hsalimi@amgen.com.
authors.forename: Hossein
authors.initials: H
authors.lastname: Salimi-Moosavi
authors.affiliation: Department of Discovery Science, Amgen, Thousand Oaks, CA 91320, USA.
authors.forename: Aaron
authors.initials: A
authors.lastname: Winters
authors.affiliation: Department of Protein Technologies, Amgen, Thousand Oaks, CA 91320, USA.
authors.forename: Christina
authors.initials: C
authors.lastname: Abbott
authors.affiliation: Department of Clinical Immunology, Amgen, Thousand Oaks, CA 91320, USA.
authors.forename: Jennifer
authors.initials: J
authors.lastname: Patel
authors.affiliation: Department of Pharmacokinetics and Drug Metabolism, Amgen, Thousand Oaks, CA 91320, USA.
authors.forename: Todd
authors.initials: T
authors.lastname: Hager
authors.affiliation: Department of Pharmacokinetics and Drug Metabolism, Amgen, Thousand Oaks, CA 91320, USA.
authors.forename: Vimal
authors.initials: V
authors.lastname: Patel
authors.affiliation: Department of Pharmacokinetics and Drug Metabolism, Amgen, Thousand Oaks, CA 91320, USA.
authors.forename: Judy
authors.initials: J
authors.lastname: Shih
authors.affiliation: Department of Clinical Immunology, Amgen, Thousand Oaks, CA 91320, USA.
authors.forename: Yao
authors.initials: Y
authors.lastname: Zhuang
authors.affiliation: Department of Pharmacokinetics and Drug Metabolism, Amgen, Thousand Oaks, CA 91320, USA.
authors.forename: Mark
authors.initials: M
authors.lastname: Ma
mesh_terms: D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology*; D057134:Antibodies, Neutralizing / Q000276:immunology* / Q000627:therapeutic use*; D001681:Biological Assay / Q000379:methods*; D006801:Humans; D007202:Indicators and Reagents; D008024:Ligands; D057230:Limit of Detection; D051379:Mice
chemical_list: D000888:Antibodies, Anti-Idiotypic; D057134:Antibodies, Neutralizing; D007202:Indicators and Reagents; D008024:Ligands
keywords: Anti-idiotypic; Antibody screening; Bioanalysis; Hybridoma; Ligand binding assay; Reagent