title: Enzymatic and acid hydrolysis of Tetraselmis suecica for polysaccharide characterization.
abstract: Carbohydrate composition of the marine microalgae, Tetraselmis suecica was characterized following acidic and enzymatic hydrolysis. Monitoring intracellular starch as a function of cultivation time at varying nitrate concentrations showed a maximum cellular starch content of 45% of dry biomass when grown under nitrate depleted conditions. Characterization of the cell wall methanolysates using GC/MS showed that the monosaccharide composition did not change in response to the nitrate concentration and consisted of 54% 3-deoxy-D-manno-oct-2-ulosonic acid (Kdo), 17% 3-deoxy-lyxo-2-heptulosaric acid (Dha), 21% galacturonic acid and 6% galactose. Presence of up to 5% Kdo in the dry weight of T. suecica established in this study demonstrates the potential of the cell wall of this species as a feedstock for Kdo, a sugar that is difficult to obtain by chemical synthesis and that has applications in medicinal chemistry.
authors.affiliation: Center for Green Chemistry and Green Engineering at Yale, 225 Prospect Street, New Haven, CT 06520, United States; Department of Chemical and Environmental Engineering, Yale University, 9 Hillhouse Avenue, New Haven, CT 06511, United States.
authors.forename: Azadeh
authors.initials: A
authors.lastname: Kermanshahi-Pour
authors.affiliation: Center for Green Chemistry and Green Engineering at Yale, 225 Prospect Street, New Haven, CT 06520, United States.
authors.forename: Toby J
authors.initials: TJ
authors.lastname: Sommer
authors.affiliation: Center for Green Chemistry and Green Engineering at Yale, 225 Prospect Street, New Haven, CT 06520, United States; School of Forestry and Environmental Studies, Yale University, 195 Prospect Street, New Haven, CT 06511, United States.
authors.forename: Paul T
authors.initials: PT
authors.lastname: Anastas
authors.affiliation: Center for Green Chemistry and Green Engineering at Yale, 225 Prospect Street, New Haven, CT 06520, United States; Department of Chemical and Environmental Engineering, Yale University, 9 Hillhouse Avenue, New Haven, CT 06511, United States; School of Forestry and Environmental Studies, Yale University, 195 Prospect Street, New Haven, CT 06511, United States. Electronic address: julie.zimmerman@yale.edu.
authors.forename: Julie B
authors.initials: JB
authors.lastname: Zimmerman
mesh_terms: D002241:Carbohydrates / Q000032:analysis* / Q000737:chemistry; D002473:Cell Wall / Q000378:metabolism; D000460:Chlorophyta / Q000737:chemistry*; D019295:Computational Biology; D008401:Gas Chromatography-Mass Spectrometry; D005087:Glucan 1,4-alpha-Glucosidase / Q000737:chemistry; D006868:Hydrolysis; D011096:Polygalacturonase / Q000737:chemistry; D059205:Renewable Energy; D000516:alpha-Amylases / Q000737:chemistry
chemical_list: D002241:Carbohydrates; D000516:alpha-Amylases; D011096:Polygalacturonase; D005087:Glucan 1,4-alpha-Glucosidase
keywords: 3-deoxy-d-manno-oct-2-ulosonic acid (Kdo); Microalgae; Pectinase; Polysaccharides; Tetraselmis suecica