title: PAMAM dendrimer with 4-carbomethoxypyrrolidone--in vitro assessment of neurotoxicity.
abstract: Cytotoxicity of cationic amino-terminated PAMAM dendrimer and modified PAMAM-pyrrolidone dendrimer was compared. LDH assay and cell visualization technique were employed. Mouse embryonic hippocampal cells (mHippoE-18) were used. The experiments were performed in FBS-deprived medium. Pyrrolidone-modification significantly diminished toxicity of PAMAM dendrimer. The absence of FBS did not reveal significant impact on the toxic effect. Results from LDH assay and MTT test were in good consistency. Low cytotoxicity of PAMAM-pyrrolidone dendrimer increases reliability of the results showing a small impact of this dendrimer on cell viability.
authors.affiliation: Department of General Biophysics, Faculty of Biology and Environmental Protection, University of Lodz, Lodz, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Janaszewska
authors.affiliation: Department of Molecular Biophysics, Faculty of Biology and Environmental Protection, University of Lodz, Lodz, Poland.
authors.forename: Maciej
authors.initials: M
authors.lastname: Studzian
authors.affiliation: Department of Chemistry, University of Copenhagen, Copenhagen, Denmark.
authors.forename: Johannes F
authors.initials: JF
authors.lastname: Petersen
authors.affiliation: Department of Chemistry, University of Copenhagen, Copenhagen, Denmark.
authors.forename: Mario
authors.initials: M
authors.lastname: Ficker
authors.affiliation: Department of Chemistry, University of Copenhagen, Copenhagen, Denmark.
authors.forename: Jørn B
authors.initials: JB
authors.lastname: Christensen
authors.affiliation: Department of General Biophysics, Faculty of Biology and Environmental Protection, University of Lodz, Lodz, Poland. Electronic address: aklajn@biol.uni.lodz.pl.
authors.forename: Barbara
authors.initials: B
authors.lastname: Klajnert-Maculewicz
mesh_terms: D000818:Animals; D050091:Dendrimers / Q000633:toxicity*; D011760:Pyrrolidinones / Q000633:toxicity*
chemical_list: D050091:Dendrimers; D011760:Pyrrolidinones
keywords: Cytotoxicity; FBS-free medium; LDH assay; MTT assay; PAMAM dendrimer; Pyrrolidone; Surface modification