title: Evaluation of in situ injectable hydrogels as controlled release device for ANXA1 derived peptide in wound healing.
abstract: In this paper, for the first time, hydrogels containing Annexin A1 N-terminal derived peptide, Ac2-26, as a novel dressing were successfully developed for dermal wound repair application. High mannuronic (M) content alginate and low molecular weight chitosan have been used as hydrogel carrier. Peptide recovery analyses, FTIR studies and molecular modelling highlighted chemical interactions between peptide and hydrogel polymers. Ac2-26 resulted entrapped into chitosan hydrogel matrix that prevented its release, whereas such interaction in alginate hydrogel slowed down peptide diffusion enabling its sustained release till 72 h. In vivo wound healing studies conducted on mice dorsal wounds indicate that after the 9th day of post wounding Ac2-26/alginate hydrogels could significantly accelerate wound healing, with complete closure of the wound on day 14th. Therefore, these results suggest that the developed of Ac2-26 high M content alginate hydrogel could be a promising wound dressing with potential application in dermal wound healing.
authors.affiliation: Department of Pharmacy, University of Salerno, via Giovanni Paolo II, 132-84084 Fisciano, SA, Italy. Electronic address: pdelgaudio@unisa.it.
authors.forename: Pasquale
authors.initials: P
authors.lastname: Del Gaudio
authors.affiliation: Department of Pharmacy, University of Salerno, via Giovanni Paolo II, 132-84084 Fisciano, SA, Italy.
authors.forename: Felicetta
authors.initials: F
authors.lastname: De Cicco
authors.affiliation: Department of Pharmacy, University of Salerno, via Giovanni Paolo II, 132-84084 Fisciano, SA, Italy.
authors.forename: Rita P
authors.initials: RP
authors.lastname: Aquino
authors.affiliation: Department of Pharmacy, University of Salerno, via Giovanni Paolo II, 132-84084 Fisciano, SA, Italy.
authors.forename: Patrizia
authors.initials: P
authors.lastname: Picerno
authors.affiliation: Department of Pharmacy, University of Salerno, via Giovanni Paolo II, 132-84084 Fisciano, SA, Italy.
authors.forename: Paola
authors.initials: P
authors.lastname: Russo
authors.affiliation: Department of Pharmacy, University of Salerno, via Giovanni Paolo II, 132-84084 Fisciano, SA, Italy.
authors.forename: Fabrizio
authors.initials: F
authors.lastname: Dal Piaz
authors.affiliation: Department of Pharmacy, University of Salerno, via Giovanni Paolo II, 132-84084 Fisciano, SA, Italy.
authors.forename: Valentina
authors.initials: V
authors.lastname: Bizzarro
authors.affiliation: Department of Pharmacy, University of Salerno, via Giovanni Paolo II, 132-84084 Fisciano, SA, Italy.
authors.forename: Raffaella
authors.initials: R
authors.lastname: Belvedere
authors.affiliation: Department of Pharmacy, University of Salerno, via Giovanni Paolo II, 132-84084 Fisciano, SA, Italy.
authors.forename: Luca
authors.initials: L
authors.lastname: Parente
authors.affiliation: Department of Pharmacy, University of Salerno, via Giovanni Paolo II, 132-84084 Fisciano, SA, Italy.
authors.forename: Antonello
authors.initials: A
authors.lastname: Petrella
mesh_terms: D000287:Administration, Topical; D000464:Alginates / Q000737:chemistry; D000818:Animals; D017305:Annexin A1 / Q000008:administration & dosage* / Q000737:chemistry; D048271:Chitosan / Q000737:chemistry; D003692:Delayed-Action Preparations / Q000008:administration & dosage / Q000737:chemistry; D065546:Drug Liberation; D020100:Hydrogels / Q000008:administration & dosage* / Q000737:chemistry; D007267:Injections; D008810:Mice, Inbred C57BL; D010455:Peptides / Q000008:administration & dosage* / Q000737:chemistry; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D000464:Alginates; D017305:Annexin A1; D003692:Delayed-Action Preparations; D020100:Hydrogels; D010455:Peptides; C096153:annexin A1 peptide (2-26); D048271:Chitosan
keywords: Alginate hydrogel; Annexin A1 derived peptide; Chitosan hydrogel; In vivo wound healing activity; Topical controlled release; Wound dressing