title: Fat-soluble vitamin intestinal absorption: absorption sites in the intestine and interactions for absorption.
abstract: The interactions occurring at the intestinal level between the fat-soluble vitamins A, D, E and K (FSVs) are poorly documented. We first determined each FSV absorption profile along the duodenal-colonic axis of mouse intestine to clarify their respective absorption sites. We then investigated the interactions between FSVs during their uptake by Caco-2 cells. Our data show that vitamin A was mostly absorbed in the mouse proximal intestine, while vitamin D was absorbed in the median intestine, and vitamin E and K in the distal intestine. Significant competitive interactions for uptake were then elucidated among vitamin D, E and K, supporting the hypothesis of common absorption pathways. Vitamin A also significantly decreased the uptake of the other FSVs but, conversely, its uptake was not impaired by vitamins D and K and even promoted by vitamin E. These results should be taken into account, especially for supplement formulation, to optimise FSV absorption.
authors.affiliation: INRA, UMR1260 "Nutrition, Obesity and Risk of Thrombosis", F-13385 Marseille, France; INSERM, UMR U1062, F-13385 Marseille, France; Aix-Marseille Université, Faculté de Médecine, F-13385 Marseille, France; Lesieur, F-92600 Asnières-sur-Seine, France.
authors.forename: Aurélie
authors.initials: A
authors.lastname: Goncalves
authors.affiliation: INRA, UMR1260 "Nutrition, Obesity and Risk of Thrombosis", F-13385 Marseille, France; INSERM, UMR U1062, F-13385 Marseille, France; Aix-Marseille Université, Faculté de Médecine, F-13385 Marseille, France.
authors.forename: Stéphanie
authors.initials: S
authors.lastname: Roi
authors.affiliation: INRA, UMR1260 "Nutrition, Obesity and Risk of Thrombosis", F-13385 Marseille, France; INSERM, UMR U1062, F-13385 Marseille, France; Aix-Marseille Université, Faculté de Médecine, F-13385 Marseille, France.
authors.forename: Marion
authors.initials: M
authors.lastname: Nowicki
authors.affiliation: Lesieur, F-92600 Asnières-sur-Seine, France.
authors.forename: Amélie
authors.initials: A
authors.lastname: Dhaussy
authors.affiliation: Lesieur, F-92600 Asnières-sur-Seine, France.
authors.forename: Alain
authors.initials: A
authors.lastname: Huertas
authors.affiliation: INRA, UMR1260 "Nutrition, Obesity and Risk of Thrombosis", F-13385 Marseille, France; INSERM, UMR U1062, F-13385 Marseille, France; Aix-Marseille Université, Faculté de Médecine, F-13385 Marseille, France.
authors.forename: Marie-Josèphe
authors.initials: MJ
authors.lastname: Amiot
authors.affiliation: INRA, UMR1260 "Nutrition, Obesity and Risk of Thrombosis", F-13385 Marseille, France; INSERM, UMR U1062, F-13385 Marseille, France; Aix-Marseille Université, Faculté de Médecine, F-13385 Marseille, France. Electronic address: Emmanuelle.Reboul@univ-amu.fr.
authors.forename: Emmanuelle
authors.initials: E
authors.lastname: Reboul
mesh_terms: D000818:Animals; D018938:Caco-2 Cells; D019587:Dietary Supplements; D006801:Humans; D007408:Intestinal Absorption; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D014801:Vitamin A / Q000493:pharmacokinetics; D014807:Vitamin D / Q000493:pharmacokinetics; D014810:Vitamin E / Q000493:pharmacokinetics; D014812:Vitamin K / Q000493:pharmacokinetics; D014815:Vitamins / Q000493:pharmacokinetics*
chemical_list: D014815:Vitamins; D014801:Vitamin A; D014812:Vitamin K; D014807:Vitamin D; D014810:Vitamin E
keywords: Cholecalciferol; Digestion; Enterocyte; Phylloquinone; Retinol; Tocopherol