title: Enhanced antitumor efficacy of vitamin E TPGS-emulsified PLGA nanoparticles for delivery of paclitaxel.
abstract: Nanoparticles are efficient delivery vehicles for cancer therapy such as paclitaxel (PTX). In this study, we formulated PTX into PLGA polymeric nanoparticles. Vitamin E TPGS was used as an emulsifier to stabilize the nanoparticle formulation. PTX was encapsulated in TPGS-emulsified polymeric nanoparticles (TENPs) by a nanoprecipitation method in ethanol-water system. The resultant PTX-TENPs showed a very uniform particle size (∼100 nm) and high drug encapsulation (>80%). The cytotoxicity of PTX-TENPs was examined in A549 lung cancer cell line. Preferential tumor accumulation of TENPs was observed in the A549 lung cancer xenograft model. Tumor growth was significantly inhibited by intravenous injection of PTX-TENPs. Our results suggested that the modified nanoprecipitation method holds great potential for the fabrication of the PTX loaded polymeric nanoparticles. TPGS can be used in the manufacture of polymeric nanoparticles for the controlled release of PTX and other anti-cancer drugs.
authors.affiliation: Department of Thoracic Surgery, First Hospital, China Medical University, Shenyang, Liaoning, PR China.
authors.forename: Yanbin
authors.initials: Y
authors.lastname: Sun
authors.affiliation: Hangzhou PushiKang Biotechnology Co., Ltd, Zhejiang, PR China.
authors.forename: Bo
authors.initials: B
authors.lastname: Yu
authors.affiliation: The State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, PR China.
authors.forename: Guoying
authors.initials: G
authors.lastname: Wang
authors.affiliation: Mudanjiang Youbo Pharmaceutical Co., Ltd, Beijing, PR China.
authors.forename: Yongsheng
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Hangzhou PushiKang Biotechnology Co., Ltd, Zhejiang, PR China.
authors.forename: Xiaomin
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Mudanjiang Youbo Pharmaceutical Co., Ltd, Beijing, PR China.
authors.forename: Yanmin
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Department of Pediatrics, PLA General Hospital, Beijing, PR China.
authors.forename: Suoqing
authors.initials: S
authors.lastname: Tang
authors.affiliation: The State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, PR China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Yuan
authors.affiliation: School of Life Sciences, Jinlin University, Changchun, PR China; College of Pharmacy, The Ohio State University, Columbus, OH, USA.
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Lee
authors.affiliation: School of Life Sciences, Jinlin University, Changchun, PR China. Electronic address: tenglesheng@jlu.edu.cn.
authors.forename: Lesheng
authors.initials: L
authors.lastname: Teng
authors.affiliation: Department of Thoracic Surgery, First Hospital, China Medical University, Shenyang, Liaoning, PR China.
authors.forename: Shun
authors.initials: S
authors.lastname: Xu
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D000431:Ethanol / Q000737:chemistry; D005260:Female; D006801:Humans; D019344:Lactic Acid / Q000737:chemistry*; D051379:Mice; D008819:Mice, Nude; D053758:Nanoparticles / Q000737:chemistry*; D017239:Paclitaxel / Q000737:chemistry* / Q000627:therapeutic use; D011092:Polyethylene Glycols / Q000737:chemistry; D011100:Polyglycolic Acid / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D014810:Vitamin E / Q000031:analogs & derivatives* / Q000737:chemistry; D014867:Water / Q000737:chemistry; D023041:Xenograft Model Antitumor Assays
chemical_list: D014867:Water; D014810:Vitamin E; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D000431:Ethanol; D011092:Polyethylene Glycols; C014225:tocophersolan; D017239:Paclitaxel
keywords: Chemotherapy; Drug delivery; Lung cancer; Nanoparticles; Paclitaxel; TPGS