title: A family of variable immunoglobulin and lectin domain containing molecules in the snail Biomphalaria glabrata.
abstract: Technical limitations have hindered comprehensive studies of highly variable immune response molecules that are thought to have evolved due to pathogen-mediated selection such as fibrinogen-related proteins (FREPs) from Biomphalaria glabrata. FREPs combine upstream immunoglobulin superfamily (IgSF) domains with a C-terminal fibrinogen-related domain (FreD) and participate in reactions against trematode parasites. From RNAseq data we assembled a de novo reference transcriptome of B. glabrata to investigate the diversity of FREP transcripts. This study increased over two fold the number of bonafide FREP subfamilies and revealed important sequence diversity within FREP12 subfamily. We also report the discovery of related molecules that feature one or two IgSF domains associated with different C-terminal lectin domains, named C-type lectin-related proteins (CREPs) and Galectin-related protein (GREP). Together, the highly similar FREPs, CREPs and GREP were designated VIgL (Variable Immunoglobulin and Lectin domain containing molecules).
authors.affiliation: CNRS, UMR 5244, Ecologie et Evolution des Interactions (2EI), Perpignan F-66860, France; Université de Perpignan Via Domitia, Perpignan F-66860, France. Electronic address: nolwenn.dheilly@stonybrook.edu.
authors.forename: Nolwenn M
authors.initials: NM
authors.lastname: Dheilly
authors.affiliation: CNRS, UMR 5244, Ecologie et Evolution des Interactions (2EI), Perpignan F-66860, France; Université de Perpignan Via Domitia, Perpignan F-66860, France.
authors.forename: David
authors.initials: D
authors.lastname: Duval
authors.affiliation: CNRS, UMR 5244, Ecologie et Evolution des Interactions (2EI), Perpignan F-66860, France; Université de Perpignan Via Domitia, Perpignan F-66860, France.
authors.forename: Gabriel
authors.initials: G
authors.lastname: Mouahid
authors.affiliation: CNRS, UMR 5244, Ecologie et Evolution des Interactions (2EI), Perpignan F-66860, France; Université de Perpignan Via Domitia, Perpignan F-66860, France.
authors.forename: Rémi
authors.initials: R
authors.lastname: Emans
authors.affiliation: CNRS, UMR 5244, Ecologie et Evolution des Interactions (2EI), Perpignan F-66860, France; Université de Perpignan Via Domitia, Perpignan F-66860, France.
authors.forename: Jean-François
authors.initials: JF
authors.lastname: Allienne
authors.affiliation: CNRS, UMR 5244, Ecologie et Evolution des Interactions (2EI), Perpignan F-66860, France; Université de Perpignan Via Domitia, Perpignan F-66860, France.
authors.forename: Richard
authors.initials: R
authors.lastname: Galinier
authors.affiliation: MGX-Montpellier GenomiX, Montpellier Genomics and Bioinformatics Facility, Montpellier F-34396, France.
authors.forename: Clémence
authors.initials: C
authors.lastname: Genthon
authors.affiliation: MGX-Montpellier GenomiX, Montpellier Genomics and Bioinformatics Facility, Montpellier F-34396, France.
authors.forename: Emeric
authors.initials: E
authors.lastname: Dubois
authors.affiliation: University of Basel, Institute of Zoology and Evolutionary Biology, Basel CH-4051, Switzerland.
authors.forename: Louis
authors.initials: L
authors.lastname: Du Pasquier
authors.affiliation: Department of Biology, Center for Evolutionary and Theoretical Immunology, University of New Mexico, Albuquerque, NM 87131, USA.
authors.forename: Coen M
authors.initials: CM
authors.lastname: Adema
authors.affiliation: CNRS, UMR 5244, Ecologie et Evolution des Interactions (2EI), Perpignan F-66860, France; Université de Perpignan Via Domitia, Perpignan F-66860, France.
authors.forename: Christoph
authors.initials: C
authors.lastname: Grunau
authors.affiliation: CNRS, UMR 5244, Ecologie et Evolution des Interactions (2EI), Perpignan F-66860, France; Université de Perpignan Via Domitia, Perpignan F-66860, France.
authors.forename: Guillaume
authors.initials: G
authors.lastname: Mitta
authors.affiliation: CNRS, UMR 5244, Ecologie et Evolution des Interactions (2EI), Perpignan F-66860, France; Université de Perpignan Via Domitia, Perpignan F-66860, France. Electronic address: benjamin.gourbal@univ-perp.fr.
authors.forename: Benjamin
authors.initials: B
authors.lastname: Gourbal
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001700:Biomphalaria / Q000235:genetics* / Q000276:immunology*; D015723:Gene Library; D007136:Immunoglobulins / Q000235:genetics* / Q000276:immunology; D037102:Lectins / Q000235:genetics* / Q000276:immunology; D008969:Molecular Sequence Data; D020641:Polymorphism, Single Nucleotide; D017422:Sequence Analysis, DNA; D059467:Transcriptome / Q000235:genetics
chemical_list: C431572:FREP2 protein, Biomphalaria glabrata; D007136:Immunoglobulins; D037102:Lectins; C445539:fibrinogen-related protein 3, Biomphalaria glabrata
keywords: C-type lectin; FREPs; Galectin; Immunoglobulin superfamily; RNAseq; VIgL