title: Heightened avidity for trisodium pyrophosphate in mice lacking Tas1r3.
abstract: Laboratory rats and mice prefer some concentrations of tri- and tetrasodium pyrophosphate (Na3HP2O7 and Na4P2O7) to water, but how they detect pyrophosphates is unknown. Here, we assessed whether T1R3 is involved. We found that relative to wild-type littermate controls, Tas1r3 knockout mice had stronger preferences for 5.6-56mM Na3HP2O7 in 2-bottle choice tests, and they licked more 17.8-56mM Na3HP2O7 in brief-access tests. We hypothesize that pyrophosphate taste in the intact mouse involves 2 receptors: T1R3 to produce a hedonically negative signal and an unknown G protein-coupled receptor to produce a hedonically positive signal; in Tas1r3 knockout mice, the hedonically negative signal produced by T1R3 is absent, leading to a heightened avidity for pyrophosphate.
authors.affiliation: Monell Chemical Senses Center, 3500 Market Street, Philadelphia, PA 19104, USA and tordoff@monell.org.
authors.forename: Michael G
authors.initials: MG
authors.lastname: Tordoff
authors.affiliation: Monell Chemical Senses Center, 3500 Market Street, Philadelphia, PA 19104, USA and.
authors.forename: Tiffany R
authors.initials: TR
authors.lastname: Aleman
authors.affiliation: Center for Medical Education, IUSM-Muncie at Ball State University, 221 N. Celia Avenue, MT 201, Muncie, IN 47306, USA.
authors.forename: Stuart A
authors.initials: SA
authors.lastname: McCaughey
mesh_terms: D000818:Animals; D002755:Choice Behavior / Q000187:drug effects*; D011756:Diphosphates / Q000494:pharmacology*; D005518:Food Preferences / Q000187:drug effects; D005838:Genotype; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D043562:Receptors, G-Protein-Coupled / Q000172:deficiency / Q000235:genetics* / Q000378:metabolism; D012965:Sodium Chloride / Q000494:pharmacology; D013649:Taste / Q000187:drug effects
chemical_list: D011756:Diphosphates; D043562:Receptors, G-Protein-Coupled; C431926:taste receptors, type 1; D012965:Sodium Chloride; C003319:sodium pyrophosphate
keywords: T1R3; intake; palatability; preference; taste