title: Protective Effect of Electroacupuncture on Neural Myelin Sheaths is Mediated via Promotion of Oligodendrocyte Proliferation and Inhibition of Oligodendrocyte Death After Compressed Spinal Cord Injury.
abstract: Electroacupuncture (EA) has been used worldwide to treat demyelinating diseases, but its therapeutic mechanism is poorly understood. In this study, a custom-designed model of compressed spinal cord injury (CSCI) was used to induce demyelination. Zusanli (ST36) and Taixi (KI3) acupoints of adult rats were stimulated by EA to demonstrate its protective effect. At 14 days after EA, both locomotor skills and ultrastructural features of myelin sheath were significantly improved. Phenotypes of proliferating cells were identified by double immunolabeling of 5-ethynyl-2'-deoxyuridine with antibodies to cell markers: NG2 [oligodendrocyte precursor cell (OPC) marker], 2',3'-cyclic-nucleotide 3'-phosphodiesterase (CNPase) (oligodendrocyte marker), and glial fibrillary acidic protein (GFAP) (astrocyte marker). EA enhanced the proliferation of OPCs and CNPase, as well as the differentiation of OPCs by promoting Olig2 (the basic helix-loop-helix protein) and attenuating Id2 (the inhibitor of DNA binding 2). EA could also improve myelin basic protein (MBP) and protect existing oligodendrocytes from apoptosis by inhibiting caspase-12 (a representative of endoplasmic reticulum stress) and cytochrome c (an apoptotic factor and hallmark of mitochondria). Therefore, our results indicate that the protective effect of EA on neural myelin sheaths is mediated via promotion of oligodendrocyte proliferation and inhibition of oligodendrocyte death after CSCI.
authors.affiliation: Traditional Chinese Medicine College, Chongqing Medical University, No.1 Medical College Road, Yuzhong District, Chongqing, 400016, China.|Institute of Neuroscience, Chongqing Medical University, No.1 Medical College Road, Yuzhong District, Chongqing, 400016, China.
authors.forename: Siqin
authors.initials: S
authors.lastname: Huang
authors.affiliation: Traditional Chinese Medicine College, Chongqing Medical University, No.1 Medical College Road, Yuzhong District, Chongqing, 400016, China.
authors.forename: Chenglin
authors.initials: C
authors.lastname: Tang
authors.affiliation: Institute of Neuroscience, Chongqing Medical University, No.1 Medical College Road, Yuzhong District, Chongqing, 400016, China. sunsq2151@cqmu.edu.cn.
authors.forename: Shanquan
authors.initials: S
authors.lastname: Sun
authors.affiliation: Traditional Chinese Medicine College, Chongqing Medical University, No.1 Medical College Road, Yuzhong District, Chongqing, 400016, China.
authors.forename: Wenfu
authors.initials: W
authors.lastname: Cao
authors.affiliation: Chongqing Three Gorgers Central Hospital, No.165 Xin Cheng Road, Wanzhou District, Chongqing, 400000, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Qi
authors.affiliation: Institute of Neuroscience, Chongqing Medical University, No.1 Medical College Road, Yuzhong District, Chongqing, 400016, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Xu
authors.affiliation: Institute of Neuroscience, Chongqing Medical University, No.1 Medical College Road, Yuzhong District, Chongqing, 400016, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Huang
authors.affiliation: Institute of Neuroscience, Chongqing Medical University, No.1 Medical College Road, Yuzhong District, Chongqing, 400016, China.
authors.forename: Weitian
authors.initials: W
authors.lastname: Lu
authors.affiliation: Institute of Neuroscience, Chongqing Medical University, No.1 Medical College Road, Yuzhong District, Chongqing, 400016, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Liu
authors.affiliation: Traditional Chinese Medicine College, Chongqing Medical University, No.1 Medical College Road, Yuzhong District, Chongqing, 400016, China.
authors.forename: Biao
authors.initials: B
authors.lastname: Gong
authors.affiliation: Traditional Chinese Medicine College, Chongqing Medical University, No.1 Medical College Road, Yuzhong District, Chongqing, 400016, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Institute of Neuroscience, Chongqing Medical University, No.1 Medical College Road, Yuzhong District, Chongqing, 400016, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Jiang
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000378:metabolism; D016923:Cell Death / Q000502:physiology*; D002454:Cell Differentiation / Q000502:physiology*; D003711:Demyelinating Diseases / Q000378:metabolism; D015671:Electroacupuncture; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D004676:Myelin Basic Protein / Q000378:metabolism; D009186:Myelin Sheath / Q000378:metabolism*; D009416:Nerve Regeneration / Q000502:physiology; D009836:Oligodendroglia / Q000166:cytology*; D017207:Rats, Sprague-Dawley; D013119:Spinal Cord Injuries / Q000378:metabolism*; D013234:Stem Cells / Q000166:cytology
chemical_list: D005904:Glial Fibrillary Acidic Protein; D004676:Myelin Basic Protein
keywords: Compressed spinal cord injury; Electroacupuncture; NG2; Oligodendrocyte; Remyelination