title: Towards high peak capacity separations in normal pressure nanoflow liquid chromatography using meter long packed capillary columns.
abstract: Single shot proteomics is a promising approach to high throughput proteomics analysis. In this strategy, long capillary columns are needed to perform long and shallow gradients to achieve high peak capacity and good peak width for informative mass spectrometric detection. Herein, we report that meter long capillary columns, packed with 5 μm particulate material, can be facilely fabricated based on single particle fritting technology. The long columns could reliably generate high peak capacities of 800 in 10 h long gradients for protein digest separations. The operation was within the pressure range (40 MPa) of the most widely used normal pressure nanoLC systems. Due to the excellent life time (>100 injections) and inter-column performance consistency, the meter long capillary columns reported here should be of practical usefulness in single shot proteomics without the need for ultra-high pressure instrumentation.
authors.affiliation: Department of Chemistry and The MOE Key Laboratory of Spectrochemical Analysis and Instrumentation, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Han
authors.affiliation: Department of Chemistry and The MOE Key Laboratory of Spectrochemical Analysis and Instrumentation, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China.
authors.forename: Linquan
authors.initials: L
authors.lastname: Ye
authors.affiliation: Department of Chemistry and The MOE Key Laboratory of Spectrochemical Analysis and Instrumentation, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China.
authors.forename: Lingjia
authors.initials: L
authors.lastname: Xu
authors.affiliation: Department of Chemistry and The MOE Key Laboratory of Spectrochemical Analysis and Instrumentation, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China.
authors.forename: Zhuoheng
authors.initials: Z
authors.lastname: Zhou
authors.affiliation: Department of Chemistry and The MOE Key Laboratory of Spectrochemical Analysis and Instrumentation, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China.
authors.forename: Fan
authors.initials: F
authors.lastname: Gao
authors.affiliation: Department of Chemistry and The MOE Key Laboratory of Spectrochemical Analysis and Instrumentation, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China.
authors.forename: Zhiliang
authors.initials: Z
authors.lastname: Xiao
authors.affiliation: Department of Chemistry and The MOE Key Laboratory of Spectrochemical Analysis and Instrumentation, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China.
authors.forename: Qiuquan
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Department of Chemistry and The MOE Key Laboratory of Spectrochemical Analysis and Instrumentation, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China. Electronic address: bozhang@xmu.edu.cn.
authors.forename: Bo
authors.initials: B
authors.lastname: Zhang
mesh_terms: D000818:Animals; D002417:Cattle; D002853:Chromatography, Liquid / Q000295:instrumentation*; D004867:Equipment Design; D011506:Proteins / Q000302:isolation & purification*; D040901:Proteomics / Q000295:instrumentation*; D012710:Serum Albumin, Bovine / Q000302:isolation & purification
chemical_list: D011506:Proteins; D012710:Serum Albumin, Bovine
keywords: Column technology; Liquid chromatography; Long capillary column; Packed column; Single particle frit; Single shot proteomics