title: MicroRNA-520g induces epithelial-mesenchymal transition and promotes metastasis of hepatocellular carcinoma by targeting SMAD7.
abstract: Hepatocellular carcinoma (HCC) is the third leading cause of cancer-related death worldwide. Aberrant expression of miRNAs contributes to HCC development. Here, we observed elevated miR-520g expression in tumor samples from HCC patients with relapse and metastasis, and this high miR-520g expression was correlated with poor survival. Through gain- and loss-of-function studies, miR-520g was demonstrated to facilitate HCC cell migration, invasion and epithelial-mesenchymal transition (EMT). SMAD7 was identified as a direct target of miR-520g. Accordingly, we conclude that high miR-520g expression promotes HCC cell mobility and EMT by targeting SMAD7, and this is correlated with reduced survival in HCC patients.
authors.affiliation: Department of Hepatobiliary Surgery, Nanfang Hospital, Southern Medical University, No. 1838 Guangzhou Road North, Guangzhou 510515, China. Electronic address: khp_smu@163.com.
authors.forename: Heping
authors.initials: H
authors.lastname: Kan
authors.affiliation: Department of Urology, The Third Affiliated Hospital, Southern Medical University, No. 183 Zhongshan Road West, Guangzhou 510000, China. Electronic address: guo_wenbin2014@163.com.
authors.forename: Wenbin
authors.initials: W
authors.lastname: Guo
authors.affiliation: Department of Hepatobiliary Surgery, Nanfang Hospital, Southern Medical University, No. 1838 Guangzhou Road North, Guangzhou 510515, China. Electronic address: huang_yuqi2014@163.com.
authors.forename: Yuqi
authors.initials: Y
authors.lastname: Huang
authors.affiliation: Department of Infectious Disease, Nanfang Hospital, Southern Medical University, No. 1838 Guangzhou Road North, Guangzhou 510515, China. Electronic address: ldl_smu@163.com.
authors.forename: Dingli
authors.initials: D
authors.lastname: Liu
mesh_terms: D000328:Adult; D000368:Aged; D006528:Carcinoma, Hepatocellular / Q000235:genetics / Q000378:metabolism* / Q000401:mortality / Q000473:pathology; D018572:Disease-Free Survival; D058750:Epithelial-Mesenchymal Transition; D005260:Female; D056945:Hep G2 Cells; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000401:mortality / Q000473:pathology; D008297:Male; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D008875:Middle Aged; D009362:Neoplasm Metastasis; D009363:Neoplasm Proteins / Q000235:genetics / Q000378:metabolism*; D012334:RNA, Neoplasm / Q000235:genetics / Q000378:metabolism*; D051906:Smad7 Protein / Q000235:genetics / Q000378:metabolism*; D015996:Survival Rate
chemical_list: C575048:MIRN520 microRNA, human; D035683:MicroRNAs; D009363:Neoplasm Proteins; D012334:RNA, Neoplasm; C497806:SMAD7 protein, human; D051906:Smad7 Protein
keywords: Cancer metastasis; Epithelial–mesenchymal transition; Hepatocellular carcinoma; MicroRNA-520g; SMAD family member 7