title: Effect of alprostadil combined with Diammonium glycyrrhizinate on renal interstitial fibrosis in SD rats.
abstract: OBJECTIVE
  To observe effect of alprostadil combined with Diammonium glycyrrhizinate on renal interstitial fibrosis in SD rats.
  
  
  METHODS
  A total of 75 SD rats were randomly divided into A, B, C, D, E groups with 15 in each group. Rats in group A served as the control group received just only but tissue separation without modeling operation, while model of unilateral ureteral obstruction (UUO) was established in B, C, D, E groups. Rats in A, B group were given saline lavage placebo treatment, while rats in C, D, E groups were given diammonium glycyrrhizinate and alprostadil injection. Five rats were sacrificed 1, 2, 3 weeks after modeling, serum creatinine level of femoral venous blood was determined. Transforming growth factor - β1 (TGF - β1) and concentration of connective tissue growth factor (CTGF) were also detected by using ELISA. Line renal interstitial tissue was taken after HE staining, renal interstitial TGF - β1 and CTGF expression were detected by using immunohistochemical method.
  
  
  RESULTS
  Serum creatinine levels of B, C, D, E group at different time points in were significantly higher than that of group A (P<0.05); serum creatinine levels in group B were significantly higher than that of C, D, E group at each time point (P<0.05). Serum creatinine level of Group E was significantly lower than C, D group after 2, 3 weeks (P<0.05). Rats in A group at each time point showed no significant changes in TGF - β1 and CREA concentration in serum and kidney tissues (P>0.05); while serum and kidney tissue TGF - β1, concentration of CREA, expression of rats in B, C, D, E groups showed a gradual increasing trend over time. TGF - β1 and CREF of Group B in serum and kidney tissues at each time point were significantly higher than that of the other groups (P<0.05). TGF - β1 and CREF of Group E in serum and kidney tissues at each time point were significantly lower than that of B, C, D group at all time points in serum and kidney tissues (P<0.05).
  
  
  CONCLUSIONS
  Alprostadil combined with diammonium glycyrrhizinate can significantly lower the expression of TGF - β1 and CTGF in serum and tissues of SD rat with renal interstitial fibrosis, thus inhibit rat renal interstitial fibrosis process. It has synergy protective effect.
authors.affiliation: Department of Pediatrics Internal Medicine, Yuhuangding Hospital, Yantai, Shandong 264000, China.
authors.forename: Wei-Yan
authors.initials: WY
authors.lastname: Cai
authors.affiliation: Department of Integrated TCM & Western Medicine, Yuhuangding Hospital, Yantai, Shandong 264000, China.
authors.forename: You-Yi
authors.initials: YY
authors.lastname: Gu
authors.affiliation: Department of Pediatrics Internal Medicine, Yuhuangding Hospital, Yantai, Shandong 264000, China. Electronic address: liaimin1965@163.com.
authors.forename: Ai-Min
authors.initials: AM
authors.lastname: Li
authors.affiliation: Department of Pediatrics Internal Medicine, Yuhuangding Hospital, Yantai, Shandong 264000, China.
authors.forename: Huan-Qin
authors.initials: HQ
authors.lastname: Cui
authors.affiliation: Department of Pediatrics Internal Medicine, Yuhuangding Hospital, Yantai, Shandong 264000, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Yao
keywords: Alprostadil; Diammonium glycyrrhizinate; Protection; Renal interstitial fibrosis