title: Long term creatine monohydrate supplementation, following neonatal hypoxic ischemic insult, improves neuromuscular coordination and spatial learning in male albino mouse.
abstract: Creatine is known to rescue animals following brain damage. Present study was designed to demonstrate the effect of long term (15 week) supplementation of 2% creatine monohydrate (Cr), following neonatal hypoxic ischemic insult, on learning and memory formation in male albino mouse. Albino mice pups were subjected to right common carotid artery ligation followed by 8% hypoxia for 25 minutes. Following weaning, animals were separated and grouped on the basis of dietry supplementation for 15 weeks followed by a battery of neurological tests including Morris water maze, open field and rota rod. It was observed that HI mice fed on 2% Cr for 15 weeks performed better than their littermates mice on normal rodent diet during water maze (learning and memory) and rotating rod (neuro-muscular coordination and balance) test while the results of open field test remained unaffected. It was also observed that Cr treated animals had a reduced brain infarct volume than untreated but this difference did not reached statistical significance. We have also observed an overall increase in body weight in Cr treated mice during the study. Over all our results are indicating that long term Cr supplementation is beneficial for male albino following hypoxic ischemic insult.
authors.affiliation: Institute of Pure and Applied Biology, Zoology Division. Bahauddin Zakariya University, Multan, Pakistan.
authors.forename: Shahid
authors.initials: S
authors.lastname: Iqbal
authors.affiliation: Institute of Molecular Biology and Biotechnology, Bahauddin Zakariya University Multan 60800, Pakistan.
authors.forename: Muhammad
authors.initials: M
authors.lastname: Ali
authors.affiliation: Institute of Pure and Applied Biology, Zoology Division. Bahauddin Zakariya University, Multan, Pakistan. Electronic address: furhan.iqbal@bzu.edu.pk.
authors.forename: Furhan
authors.initials: F
authors.lastname: Iqbal
mesh_terms: D000818:Animals; D001835:Body Weight / Q000187:drug effects; D002340:Carotid Artery Diseases; D003401:Creatine / Q000008:administration & dosage*; D004195:Disease Models, Animal; D020925:Hypoxia-Ischemia, Brain / Q000178:diet therapy* / Q000503:physiopathology / Q000523:psychology; D008297:Male; D018782:Maze Learning; D051379:Mice; D009069:Movement Disorders / Q000178:diet therapy* / Q000503:physiopathology; D018696:Neuroprotective Agents / Q000008:administration & dosage*; D045442:Rotarod Performance Test
chemical_list: D018696:Neuroprotective Agents; D003401:Creatine
keywords: Albino mice; Creatine; Hypoxia ischemia; Morris water maze; Open field; Rota rod