title: Life-long in vivo cell-lineage tracing shows that no oogenesis originates from putative germline stem cells in adult mice.
abstract: Whether or not oocyte regeneration occurs in adult life has been the subject of much debate. In this study, we have traced germ-cell lineages over the life spans of three genetically modified mouse models and provide direct evidence that oogenesis does not originate from any germline stem cells (GSCs) in adult mice. By selective ablation of all existing oocytes in a Gdf9-Cre;iDTR mouse model, we have demonstrated that no new germ cells were ever regenerated under pathological conditions. By in vivo tracing of oocytes and follicles in the Sohlh1-CreER(T2);R26R and Foxl2-CreER(T2);mT/mG mouse models, respectively, we have shown that the initial pool of oocytes is the only source of germ cells throughout the life span of the mice and that no adult oogenesis ever occurs under physiological conditions. Our findings clearly show that there are no GSCs that contribute to adult oogenesis in mice and that the initial pool of oocytes formed in early life is the only source of germ cells throughout the entire reproductive life span.
authors.affiliation: Department of Chemistry and Molecular Biology, University of Gothenburg, SE-405 30 Gothenburg, Sweden;
authors.forename: Hua
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Cancer Center, Qilu Hospital of Shandong University, 250012 Shandong, China;
authors.forename: Lian
authors.initials: L
authors.lastname: Liu
authors.affiliation: Department of Chemistry and Molecular Biology, University of Gothenburg, SE-405 30 Gothenburg, Sweden;
authors.forename: Xin
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Chemistry and Molecular Biology, University of Gothenburg, SE-405 30 Gothenburg, Sweden;
authors.forename: Kiran
authors.initials: K
authors.lastname: Busayavalasa
authors.affiliation: Department of Chemistry and Molecular Biology, University of Gothenburg, SE-405 30 Gothenburg, Sweden;
authors.forename: Yan
authors.initials: Y
authors.lastname: Shen
authors.affiliation: Division of Obstetrics and Gynecology, Department of Clinical Sciences, Intervention and Technology, Karolinska Institute and Karolinska University Hospital, Huddinge, SE-141 86 Stockholm, Sweden; and.
authors.forename: Outi
authors.initials: O
authors.lastname: Hovatta
authors.affiliation: Center for Nuclear Receptors and Cell Signaling, University of Houston, Houston, TX 77204 jgustafs@central.uh.edu kui.liu@gu.se.
authors.forename: Jan-Åke
authors.initials: JÅ
authors.lastname: Gustafsson
authors.affiliation: Department of Chemistry and Molecular Biology, University of Gothenburg, SE-405 30 Gothenburg, Sweden; jgustafs@central.uh.edu kui.liu@gu.se.
authors.forename: Kui
authors.initials: K
authors.lastname: Liu
mesh_terms: D053687:Adult Stem Cells / Q000166:cytology*; D000704:Analysis of Variance; D000818:Animals; D030801:Animals, Genetically Modified; D019070:Cell Lineage / Q000502:physiology*; D003433:Crosses, Genetic; D005260:Female; D020869:Gene Expression Profiling; D006652:Histological Techniques; D051379:Mice; D008817:Mice, Mutant Strains; D009865:Oocytes / Q000502:physiology*; D009866:Oogenesis / Q000502:physiology*; D013629:Tamoxifen
chemical_list: D013629:Tamoxifen
keywords: follicle tracing; genetically modified mouse models; life-long observations; no postnatal oocyte regeneration; oocyte tracing