title: Protective effect of 2-APB on testicular ischemia-reperfusion injury in rats.
abstract: OBJECTIVE
  We performed biochemical and histopathological evaluations to assess the effects of 2-APB on ischemia-reperfusion induced testicular damage.
  
  
  METHODS
  A total of 28 rats were randomly divided into 4 groups, including sham treated, ischemia-reperfusion, ischemia-reperfusion plus 2 mg/kg 2-APB and ischemia-reperfusion plus 4 mg/kg 2-APB. Testicular tissue superoxide dismutase, glutathione, malondialdehyde, total antioxidant capacity and DNA fragmentation levels were determined. Testicular tissue samples were examined by histopathology and TUNEL staining.
  
  
  RESULTS
  Mean superoxide dismutase, total antioxidant capacity and glutathione were significantly higher in the sham treated group than in the ischemia-perfusion group (p <0.05). Mean malondialdehyde and DNA fragmentation levels were significantly lower in the sham treated group than in the ischemia-reperfusion group (p <0.05). After 2-APB treatment superoxide dismutase, total antioxidant capacity and glutathione were significantly increased but malondialdehyde and DNA fragmentation levels were significantly decreased compared to the ischemia-reperfusion group (p <0.05). The number of TUNEL positive cells was significantly lower in the 2-APB treatment groups than in the ischemia-reperfusion group (p <0.05).
  
  
  CONCLUSIONS
  In rats 2-APB reduced the oxidative stress and apoptosis caused by testicular ischemia-reperfusion injury. The testicular protective effect of 2-APB appears to be mediated through its antiapoptotic and antioxidative effects.
authors.affiliation: Department of Urology, Faculty of Medicine, Veterinary Faculty, Balikesir University, Balikesir, Turkey. Electronic address: sarierhan@yahoo.com.
authors.forename: Erhan
authors.initials: E
authors.lastname: Sari
authors.affiliation: Department of Biochemistry, Veterinary Faculty, Balikesir University, Balikesir, Turkey.
authors.forename: Hasan
authors.initials: H
authors.lastname: Aksit
authors.affiliation: Department of Physiology, Faculty of Medicine, Veterinary Faculty, Balikesir University, Balikesir, Turkey.
authors.forename: Haydar Ali
authors.initials: HA
authors.lastname: Erken
authors.affiliation: Department of Histology and Embryology, Faculty of Medicine, Erciyes University, Kayseri, Turkey.
authors.forename: Arzu
authors.initials: A
authors.lastname: Yay
authors.affiliation: Department of Pharmacology, Veterinary Faculty, Balikesir University, Balikesir, Turkey.
authors.forename: Dilek
authors.initials: D
authors.lastname: Aksit
authors.affiliation: Department of Urology, Faculty of Medicine, Adnan Menderes University, Aydin, Turkey.
authors.forename: Akin Soner
authors.initials: AS
authors.lastname: Amasyali
authors.affiliation: Department of Biochemistry, Veterinary Faculty, Balikesir University, Balikesir, Turkey.
authors.forename: Onur
authors.initials: O
authors.lastname: Yildiz
authors.affiliation: Department of Urology, Faculty of Medicine, Veterinary Faculty, Balikesir University, Balikesir, Turkey.
authors.forename: Ahmet
authors.initials: A
authors.lastname: Kucukyangoz
authors.affiliation: Department of Histology and Embryology, Faculty of Medicine, Erciyes University, Kayseri, Turkey.
authors.forename: Derya
authors.initials: D
authors.lastname: Akkus
authors.affiliation: Department of Biochemistry, Faculty of Medicine, Veterinary Faculty, Balikesir University, Balikesir, Turkey.
authors.forename: Kamil
authors.initials: K
authors.lastname: Seyrek
mesh_terms: D000818:Animals; D001896:Boron Compounds / Q000627:therapeutic use*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000517:prevention & control*; D013737:Testis / Q000098:blood supply*
chemical_list: D001896:Boron Compounds; C109986:2-aminoethoxydiphenyl borate
keywords: 2-aminoethoxydiphenyl borate; apoptosis; oxidative stress; reperfusion injury; testis