title: Influence of the extraction time on macromolecular parameters of galactomannans.
abstract: This study evaluated the aqueous extraction of galactomannans from the seeds of Mimosa scabrella (GM), Stryphnodendron adstringens (GS) and Schizolobium parahybae (GG) for 1, 2, 3, 4, 6, 24 and 48 h. The efficiency of extraction processes was assessed in terms of yield, carbohydrate and protein content. The extraction process, as well as the source of the galactomananns generated molecules with differences in molar mass, viscosity and rigidity analyzed by HPSEC-MALLS/RI/VIS. The extraction time results for each species, based on minimum extraction time and HPSEC-MALLS/RI/VIS results, were 4 h (GM4h), 6 h (GS6h) and 2 h (GG2h) for GM, GS and GG, respectively. In most cases, the apparent persistence length, as determined by viscometry, indicated that aggregates remained in galactomannans after centrifugation and filtration. Results suggest an effective extraction time for each plant source of galactomannan based on its performance and its macromolecular behavior in solution.
authors.affiliation: Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, P.B.19046, Centro Politécnico, CEP 81531-990 Curitiba, PR, Brasil.
authors.forename: Marina de Oliveira
authors.initials: Mde O
authors.lastname: Salvalaggio
authors.affiliation: Departamento de Química, Universidade Federal do Paraná, P.B.19046, Centro Politécnico, CEP 81531-990 Curitiba, PR, Brasil.
authors.forename: Rilton Alves
authors.initials: RA
authors.lastname: de Freitas
authors.affiliation: Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, P.B.19046, Centro Politécnico, CEP 81531-990 Curitiba, PR, Brasil.
authors.forename: Elvis Marcelo
authors.initials: EM
authors.lastname: Franquetto
authors.affiliation: Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, P.B.19046, Centro Politécnico, CEP 81531-990 Curitiba, PR, Brasil.
authors.forename: Heidegrid Siebert
authors.initials: HS
authors.lastname: Koop
authors.affiliation: Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, P.B.19046, Centro Politécnico, CEP 81531-990 Curitiba, PR, Brasil. Electronic address: jlms12@yahoo.com.
authors.forename: Joana Léa Meira
authors.initials: JL
authors.lastname: Silveira
mesh_terms: D007887:Fabaceae / Q000737:chemistry*; D005374:Filtration; D005690:Galactose / Q000032:analysis / Q000031:analogs & derivatives; D008351:Mannans / Q000737:chemistry / Q000302:isolation & purification*; D008358:Mannose / Q000032:analysis; D008970:Molecular Weight; D012542:Scattering, Radiation; D012639:Seeds / Q000737:chemistry*; D014783:Viscosity
chemical_list: D008351:Mannans; C012990:galactomannan; D008358:Mannose; D005690:Galactose
keywords: Galactomannan; High-performance size-exclusion chromatography coupled to multidetector (HPSEC-MALLS/RI/VIS); Mimosa scabrella; Persistence length; Schizolobium parahybae; Stryphnodendron adstringens