title: Flow-modulation low-pressure comprehensive two-dimensional gas chromatography.
abstract: The present paper contains research data relative to an approach herein defined as flow-modulation (FM) low-pressure comprehensive two-dimensional gas chromatography, abbreviated as "GC×LP GC". The abbreviation LP is positioned before the second GC abbreviation because LP conditions were generated across a mega-bore second-dimension column (10m×0.53mm ID), it being connected to a quadrupole mass spectrometer (qMS). Flow modulation was performed with MS-compatible gas flows (7-8mL/min), following recent research work [7]; using such an approach, the main disadvantage of flow modulation, specifically the generation of excessively high second-dimension flows, is avoided. A further noteworthy aspect of the investigation was the use of a long accumulation loop (e.g., 51cm), a modification that greatly improved the general post-modulation peak shape quality. FM GC×LP GC-qMS applications on pure standard compounds, as well as on milk and fish oil fatty acid methyl esters, are shown and discussed.
authors.affiliation: Dipartimento di Scienze del Farmaco e Prodotti per la Salute, Università di Messina, viale Annunziata, 98168 Messina, Italy.
authors.forename: Peter Q
authors.initials: PQ
authors.lastname: Tranchida
authors.affiliation: Dipartimento di Scienze del Farmaco e Prodotti per la Salute, Università di Messina, viale Annunziata, 98168 Messina, Italy.
authors.forename: Flavio A
authors.initials: FA
authors.lastname: Franchina
authors.affiliation: Dipartimento di Scienze del Farmaco e Prodotti per la Salute, Università di Messina, viale Annunziata, 98168 Messina, Italy; Centro Integrato di Ricerca (C.I.R.), Università Campus Bio-Medico, Via Álvaro del Portillo, 21, 00128 Roma, Italy; Chromaleont s.r.l. A start-up of the University of Messina, c/o Dipartimento di Scienze del Farmaco e Prodotti per la Salute, Università di Messina, viale Annunziata, 98168 Messina, Italy.
authors.forename: Paola
authors.initials: P
authors.lastname: Dugo
authors.affiliation: Dipartimento di Scienze del Farmaco e Prodotti per la Salute, Università di Messina, viale Annunziata, 98168 Messina, Italy; Centro Integrato di Ricerca (C.I.R.), Università Campus Bio-Medico, Via Álvaro del Portillo, 21, 00128 Roma, Italy; Chromaleont s.r.l. A start-up of the University of Messina, c/o Dipartimento di Scienze del Farmaco e Prodotti per la Salute, Università di Messina, viale Annunziata, 98168 Messina, Italy. Electronic address: lmondello@unime.it.
authors.forename: Luigi
authors.initials: L
authors.lastname: Mondello
keywords: Comprehensive two-dimensional gas chromatography; Fatty acid methyl esters; Fish oil; Flow modulation; Milk fat; Quadrupole mass spectrometry