title: Chemical and biological comparison of the fruit extracts of Citrus wilsonii Tanaka and Citrus medica L.
abstract: Citri Fructus (CF), the mature fruit of Citrus wilsonii Tanaka (CWT) or Citrus medica L. (CML), is an important citrus by-product with health promoting and nutritive properties. The present study compares the chemical and biological differences of CWT and CML. Thin layer chromatography and high performance liquid chromatography, coupled with quadrupole time-of-flight tandem mass spectrometry techniques, were employed to compare the chemical profiles of CWT and CML. A total of 25 compounds were identified and the results indicated that there were significant differences in chemical composition between the two CF species. The quantitative results obtained by HPLC coupled with diode array detector method demonstrated that naringin was present in the highest amounts in CWT, whilst nomilin was the most dominant constituent in CML. It was also found that CWT had significantly higher free radical-scavenging activity than CML.
authors.affiliation: State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing, PR China.
authors.forename: Pan
authors.initials: P
authors.lastname: Zhao
authors.affiliation: State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing, PR China.
authors.forename: Li
authors.initials: L
authors.lastname: Duan
authors.affiliation: State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing, PR China.
authors.forename: Long
authors.initials: L
authors.lastname: Guo
authors.affiliation: State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing, PR China.
authors.forename: Li-Li
authors.initials: LL
authors.lastname: Dou
authors.affiliation: State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing, PR China.
authors.forename: Xin
authors.initials: X
authors.lastname: Dong
authors.affiliation: State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing, PR China.
authors.forename: Ping
authors.initials: P
authors.lastname: Zhou
authors.affiliation: State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing, PR China. Electronic address: liping2004@126.com.
authors.forename: Ping
authors.initials: P
authors.lastname: Li
authors.affiliation: State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing, PR China. Electronic address: liuehu2011@163.com.
authors.forename: E-Hu
authors.initials: EH
authors.lastname: Liu
mesh_terms: D000975:Antioxidants / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002957:Citrus / Q000737:chemistry*; D005638:Fruit / Q000737:chemistry*; D010936:Plant Extracts / Q000032:analysis*; D053719:Tandem Mass Spectrometry / Q000379:methods
chemical_list: D000975:Antioxidants; D010936:Plant Extracts
keywords: Antioxidant activity; Citrus medica L.; Citrus wilsonii Tanaka; HPLC–DAD; HPLC–QTOF/MS