title: Rich bacterial assemblages from Maritime Antarctica (Potter Cove, South Shetlands) reveal several kinds of endemic and undescribed phylotypes.
abstract: Bacterial richness in maritime Antarctica has been poorly described to date. Phylogenetic affiliation of seawater free-living microbial assemblages was studied from three locations near the Argentinean Jubany Station during two Antarctic summers. Sixty 16S RNA cloned sequences were phylogenetically affiliated to Alphaproteobacteria (30/60 clones), Gammaproteobacteria(19/60 clones), Betaproteobacteria and Cytophaga-Flavobacteriia-Bacteroides (CFB), which were (2/60) and (3/60) respectively. Furthermore, six out of 60 clones could not be classified. Both, Alphaproteobacteria and Gammaproteobacteria, showed several endemic and previously undescribed sequences. Moreover, the absence of Cyanobacteria sequences in our samples is remarkable. In conclusion, we are reporting a rich sequence assemblage composed of widely divergent isolates among themselves and distant from the most closely related sequences currently deposited in data banks.
authors.affiliation: Cátedra de Virología, FFyB, Universidad de Buenos Aires, Buenos Aires, Argentina.
authors.forename: Ignacio A
authors.initials: IA
authors.lastname: Landone Vescovo
authors.affiliation: Cátedra de Virología, FFyB, Universidad de Buenos Aires, Buenos Aires, Argentina.
authors.forename: Marcelo D
authors.initials: MD
authors.lastname: Golemba
authors.affiliation: Cátedra de Virología, FFyB, Universidad de Buenos Aires, Buenos Aires, Argentina.
authors.forename: Federico A
authors.initials: FA
authors.lastname: Di Lello
authors.affiliation: Cátedra de Virología, FFyB, Universidad de Buenos Aires, Buenos Aires, Argentina.
authors.forename: Andrés C A
authors.initials: AC
authors.lastname: Culasso
authors.affiliation: Cátedra de Microbiología Industrial y Biotecnología, FFyB, Universidad de Buenos Aires, Argentina.
authors.forename: Gustavo
authors.initials: G
authors.lastname: Levin
authors.affiliation: Cátedra de Microbiología Industrial y Biotecnología, FFyB, Universidad de Buenos Aires, Argentina; Instituto Antártico Argentino, Buenos Aires, Argentina.
authors.forename: Lucas
authors.initials: L
authors.lastname: Ruberto
authors.affiliation: Cátedra de Microbiología Industrial y Biotecnología, FFyB, Universidad de Buenos Aires, Argentina; Instituto Antártico Argentino, Buenos Aires, Argentina.
authors.forename: Walter P
authors.initials: WP
authors.lastname: Mac Cormack
authors.affiliation: Cátedra de Virología, FFyB, Universidad de Buenos Aires, Buenos Aires, Argentina. Electronic address: jlopez@ffyb.uba.ar.
authors.forename: José L
authors.initials: JL
authors.lastname: López
mesh_terms: D000864:Antarctic Regions; D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D001483:Base Sequence; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D019143:Evolution, Molecular; D064307:Microbiota; D008969:Molecular Sequence Data; D010802:Phylogeny; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D021521:Ribotyping; D012623:Seawater / Q000382:microbiology*
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S
keywords: Afiliación filogenética; Antártica; Antártida; Bacterial assemblages; Colecciones bacterianas; Phylogenetic affiliation