title: Fluorescence reports intact quantum dot uptake into roots and translocation to leaves of Arabidopsis thaliana and subsequent ingestion by insect herbivores.
abstract: We explored the impact of quantum dot (QD) coat characteristics on NP stability, uptake, and translocation in Arabidopsis thaliana, and subsequent transfer to primary consumers, Trichoplusia ni (T. ni). Arabidopsis was exposed to CdSe/CdZnS QDs with three different coatings: Poly(acrylic acid-ethylene glycol) (PAA-EG), polyethylenimine (PEI) and poly(maleic anhydride-alt-1-octadecene)-poly(ethylene glycol) (PMAO-PEG), which are anionic, cationic, and relatively neutral, respectively. PAA-EG-coated QDs were relatively stable and taken up from a hydroponic medium through both Arabidopsis leaf petioles and roots, without apparent aggregation, and showed generally uniform distribution in leaves. In contrast, PEI- and PMAO-PEG-coated QDs displayed destabilization in the hydroponic medium, and generated particulate fluorescence plant tissues, suggesting aggregation. PAA-EG QDs moved faster than PEI QDs through leaf petioles; however, 8-fold more cadmium accumulated in PEI QD-treated leaves than in those exposed to PAA-EG QDs, possibly due to PEI QD dissolution and direct metal uptake. T. ni caterpillars that fed on Arabidopsis exposed to QDs had reduced performance, and QD fluorescence was detected in both T. ni bodies and frass, demonstrating trophic transfer of intact QDs from plants to insects. Overall, this paper demonstrates that QD coat properties influence plant nanoparticle uptake and translocation and can impact transfer to herbivores.
authors.affiliation: Department of BioSciences, ‡Department of Civil & Environmental Engineering, and §Department of Chemistry, Rice University , Houston, Texas 77005, United States.
authors.forename: Yeonjong
authors.initials: Y
authors.lastname: Koo
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.forename: Qingbo
authors.initials: Q
authors.lastname: Zhang
authors.forename: Huiguang
authors.initials: H
authors.lastname: Zhu
authors.forename: E Wassim
authors.initials: EW
authors.lastname: Chehab
authors.forename: Vicki L
authors.initials: VL
authors.lastname: Colvin
authors.forename: Pedro J J
authors.initials: PJ
authors.lastname: Alvarez
authors.forename: Janet
authors.initials: J
authors.lastname: Braam
mesh_terms: D000818:Animals; D017360:Arabidopsis / Q000378:metabolism*; D002104:Cadmium / Q000378:metabolism; D005453:Fluorescence; D060434:Herbivory / Q000502:physiology*; D007313:Insecta / Q000502:physiology*; D007814:Larva / Q000502:physiology; D018613:Microscopy, Confocal; D018515:Plant Leaves / Q000378:metabolism*; D018517:Plant Roots / Q000378:metabolism*; D045663:Quantum Dots / Q000378:metabolism*; D012643:Selenium / Q000378:metabolism
chemical_list: D002104:Cadmium; D012643:Selenium