title: Intact cell mass spectrometry as a progress tracking tool for batch and fed-batch fermentation processes.
abstract: Penicillin production during a fermentation process using industrial strains of Penicillium chrysogenum is a research topic permanently discussed since the accidental discovery of the antibiotic. Intact cell mass spectrometry (ICMS) can be a fast and novel monitoring tool for the fermentation progress during penicillin V production in a nearly real-time fashion. This method is already used for the characterization of microorganisms and the differentiation of fungal strains; therefore, the application of ICMS to samples directly harvested from a fermenter is a promising possibility to get fast information about the progress of fungal growth. After the optimization of the ICMS method to penicillin V fermentation broth samples, the obtained ICMS data were evaluated by hierarchical cluster analysis or an in-house software solution written especially for ICMS data comparison. Growth stages of a batch and fed-batch fermentation of Penicillium chrysogenum are differentiated by one of those statistical approaches. The application of two matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) instruments in the linear positive ion mode from different vendors demonstrated the universal applicability of the developed ICMS method. The base for a fast and easy-to-use method for monitoring the fermentation progress of P. chrysogenum is created with this ICMS method developed especially for fermentation broth samples.
authors.affiliation: Institute of Chemical Technologies and Analytics, Vienna University of Technology, A-1060 Vienna, Austria.
authors.forename: Michaela
authors.initials: M
authors.lastname: Helmel
authors.affiliation: Institute of Chemical Technologies and Analytics, Vienna University of Technology, A-1060 Vienna, Austria.
authors.forename: Martina
authors.initials: M
authors.lastname: Marchetti-Deschmann
authors.affiliation: Department of Protein Biochemistry and Proteomics, Centrum of the Region Hana for Biotechnological and Agricultural Research, Faculty of Sciences, Palacký University, CZ-78371 Olomouc, Czech Republic.
authors.forename: Martin
authors.initials: M
authors.lastname: Raus
authors.affiliation: Christian Doppler Laboratory for Mechanistic and Physiological Methods for Improved Bioprocesses, Institute of Chemical Engineering, Vienna University of Technology, A-1060 Vienna, Austria.
authors.forename: Andreas E
authors.initials: AE
authors.lastname: Posch
authors.affiliation: Christian Doppler Laboratory for Mechanistic and Physiological Methods for Improved Bioprocesses, Institute of Chemical Engineering, Vienna University of Technology, A-1060 Vienna, Austria.
authors.forename: Christoph
authors.initials: C
authors.lastname: Herwig
authors.affiliation: Department of Protein Biochemistry and Proteomics, Centrum of the Region Hana for Biotechnological and Agricultural Research, Faculty of Sciences, Palacký University, CZ-78371 Olomouc, Czech Republic.
authors.forename: Marek
authors.initials: M
authors.lastname: Šebela
authors.affiliation: Institute of Chemical Technologies and Analytics, Vienna University of Technology, A-1060 Vienna, Austria. Electronic address: guenter.allmaier@tuwien.ac.at.
authors.forename: Günter
authors.initials: G
authors.lastname: Allmaier
mesh_terms: D061249:Batch Cell Culture Techniques / Q000295:instrumentation / Q000379:methods*; D005285:Fermentation; D013058:Mass Spectrometry; D010408:Penicillium chrysogenum / Q000166:cytology / Q000254:growth & development; D013997:Time Factors
keywords: Batch; Fed-batch; Fermentation; Intact cell mass spectrometry; Penicillin V; Penicillium chrysogenum