title: Cascade signal amplification for electrochemical immunosensing by integrating biobarcode probes, surface-initiated enzymatic polymerization and silver nanoparticle deposition.
abstract: A cascade signal amplification strategy through combining surface-initiated enzymatic polymerization (SIEP) and the subsequent deposition of strepavidin functionalized silver nanoparticles (AgNPs) was proposed. The first step of constructing the electrochemical immunosensor involves covalently immobilizing capture antibody on a chitosan modified glass carbon electrode, which then catalyzes DNA addition of deoxynucleotides (dNTP) at the 3'-OH group by terminal deoxynucleotidyl transferase (TdT), leading to the formation of long single-stranded DNAs labeled with numerous biotins. Following the deposition of numerous strepavidin functionalized AgNPs on those long DNA chains, electrochemical stripping signal of silver was used to monitor the immunoreaction in KCl solution. Using α-fetoprotein as a model analyte, this amplification strategy could detect fetoprotein down to 0.046pg/mL with a wide linear range from 0.1pg/mL to 1.0ng/mL. The achieved high sensitivity and good reproducibility suggest that this cascade signal amplification strategy has great potential for detecting biological samples and possibly clinical application.
authors.affiliation: College of Chemistry and Materials Engineering, Wenzhou University, Wenzhou 325037, PR China; State Key Laboratory of Analytical Chemistry for Life Science, Nanjing University, Nanjing 210093, PR China. Electronic address: lindj@wzu.edu.cn.
authors.forename: Dajie
authors.initials: D
authors.lastname: Lin
authors.affiliation: College of Chemistry and Materials Engineering, Wenzhou University, Wenzhou 325037, PR China.
authors.forename: Chengyang
authors.initials: C
authors.lastname: Mei
authors.affiliation: College of Chemistry and Materials Engineering, Wenzhou University, Wenzhou 325037, PR China.
authors.forename: Aili
authors.initials: A
authors.lastname: Liu
authors.affiliation: College of Chemistry and Materials Engineering, Wenzhou University, Wenzhou 325037, PR China.
authors.forename: Huile
authors.initials: H
authors.lastname: Jin
authors.affiliation: College of Chemistry and Materials Engineering, Wenzhou University, Wenzhou 325037, PR China. Electronic address: shunwang@wzu.edu.cn.
authors.forename: Shun
authors.initials: S
authors.lastname: Wang
authors.affiliation: College of Chemistry and Materials Engineering, Wenzhou University, Wenzhou 325037, PR China; Department of Chemistry and Biochemistry, University of Windsor, ON, Canada N9B 3P4.
authors.forename: Jichang
authors.initials: J
authors.lastname: Wang
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D048271:Chitosan / Q000737:chemistry; D004253:DNA Nucleotidylexotransferase / Q000737:chemistry; D004277:DNA, Single-Stranded / Q000737:chemistry*; D055664:Electrochemical Techniques; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry*; D012834:Silver / Q000737:chemistry; D019809:Streptavidin / Q000737:chemistry; D013499:Surface Properties; D000509:alpha-Fetoproteins / Q000302:isolation & purification*
chemical_list: D004277:DNA, Single-Stranded; D000509:alpha-Fetoproteins; D012834:Silver; D048271:Chitosan; D019809:Streptavidin; D004253:DNA Nucleotidylexotransferase
keywords: Anodic stripping; Biobarcode probe; Cascade signal amplification; Immunosensor; Silver nanoparticles; Surface-initiated enzymatic polymerization