title: NiCoBP-doped carbon nanotube hybrid: a novel oxidase mimetic system for highly efficient electrochemical immunoassay.
abstract: NiCoBP-doped multi-walled carbon nanotube (NiCoBP-MWCNT) was first synthesized by using induced electroless-plating method and functionalized with the biomolecules for highly efficient electrochemical immunoassay of prostate-specific antigen (PSA, used as a model analyte). We discovered that the as-synthesized NiCoBP-MWCNT had the ability to catalyze the glucose oxidization with a stable and well-defined redox peak. The catalytic current increased with the increment of the immobilized NiCoBP-MWCNT on the electrode. Transmission electron microscope (TEM) and energy dispersive X-ray spectrometry (EDX) were employed to characterize the as-prepared NiCoBP-MWCNT. Using the NiCoBP-MWCNT-conjugated anti-PSA antibody as the signal-transduction tag, a new enzyme-free electrochemical immunoassay protocol could be designed for the detection of target PSA on the capture antibody-functionalized immunosensing interface. Experimental results revealed that the designed immunoassay system could exhibit good electrochemical responses toward target PSA, and allowed the detection of PSA at a concentration as low as 0.035ngmL(-1). More importantly, the NiCoBP-MWCNT-based oxidase mimetic system could be further extended for the monitoring of other low-abundance proteins or disease-related biomarkers by tuning the target antibody.
authors.affiliation: Institute of Nanomedicine and Nanobiosensing, Department of Chemistry, Fuzhou University, Fuzhou 350108, People's Republic of China.
authors.forename: Bing
authors.initials: B
authors.lastname: Zhang
authors.affiliation: Institute of Nanomedicine and Nanobiosensing, Department of Chemistry, Fuzhou University, Fuzhou 350108, People's Republic of China.
authors.forename: Yu
authors.initials: Y
authors.lastname: He
authors.affiliation: Institute of Nanomedicine and Nanobiosensing, Department of Chemistry, Fuzhou University, Fuzhou 350108, People's Republic of China.
authors.forename: Bingqian
authors.initials: B
authors.lastname: Liu
authors.affiliation: Institute of Nanomedicine and Nanobiosensing, Department of Chemistry, Fuzhou University, Fuzhou 350108, People's Republic of China. Electronic address: dianping.tang@fzu.edu.cn.
authors.forename: Dianping
authors.initials: D
authors.lastname: Tang
mesh_terms: D040761:Biomimetic Materials / Q000737:chemistry*; D001896:Boron Compounds / Q000737:chemistry*; D003035:Cobalt / Q000737:chemistry*; D004553:Electric Conductivity; D004563:Electrochemistry; D005947:Glucose / Q000737:chemistry; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D008958:Models, Molecular; D008968:Molecular Conformation; D037742:Nanotubes, Carbon / Q000737:chemistry*; D009532:Nickel / Q000737:chemistry*; D010088:Oxidoreductases / Q000378:metabolism*; D017430:Prostate-Specific Antigen / Q000097:blood; D015203:Reproducibility of Results
chemical_list: D001896:Boron Compounds; D037742:Nanotubes, Carbon; D003035:Cobalt; D009532:Nickel; D010088:Oxidoreductases; D017430:Prostate-Specific Antigen; D005947:Glucose
keywords: Carbon nanotube; Electrochemical immunoassay; NiCoBP nanoparticles; Oxidase mimetic system; Prostate-specific antigen