title: A novel detachable head-mounted device for simultaneous EEG and photoacoustic monitoring of epilepsy in freely moving rats.
abstract: The study of neuro-hemodynamic changes in freely moving animals provides for a better understanding of brain dynamics in normal and disease states. While it has been shown that hemodynamic changes are closely related to seizures, methods for detection in freely moving animals are limited. In this work, we integrate photoacoustic sensor technology and electroencephalography into a small portable device that can be attached on the head of wake freely moving animals. We demonstrate chronic simultaneous monitoring of photoacoustic and electroencephalographic signals in an acute seizure model of epilepsy. Our results demonstrate that both the neural and vascular responses during seizures in freely moving rats have characteristics which are observed to be different and more diverse from that of anesthetized rats. This implies that the neurovascular coupling in seizure in free moving animals are more complicated, which calls for more detailed study in future. To the best of our knowledge, this is the first time for hemodynamic monitoring of seizure in free moving animals. This technology also promises for other hemodynamic related research study in freely moving small animals.
authors.affiliation: College of Biology, Hunan University, Changsha, Hunan 410082, China; J. Crayton Pruitt Family Department of Biomedical Engineering, University of Florida, Gainesville, FL 32611, USA.
authors.forename: Bo
authors.initials: B
authors.lastname: Wang
authors.affiliation: Department of Pediatrics, College of Medicine, University of Florida, Gainesville, FL 32610, USA; Department of Neurology, College of Medicine, University of Florida, Gainesville, FL 32610, USA; Department of Neuroscience, College of Medicine, University of Florida, Gainesville, FL 32610, USA.
authors.forename: Junli
authors.initials: J
authors.lastname: Zhou
authors.affiliation: J. Crayton Pruitt Family Department of Biomedical Engineering, University of Florida, Gainesville, FL 32611, USA; Department of Pediatrics, College of Medicine, University of Florida, Gainesville, FL 32610, USA; Department of Neurology, College of Medicine, University of Florida, Gainesville, FL 32610, USA; Department of Neuroscience, College of Medicine, University of Florida, Gainesville, FL 32610, USA.
authors.forename: Paul
authors.initials: P
authors.lastname: Carney
authors.affiliation: J. Crayton Pruitt Family Department of Biomedical Engineering, University of Florida, Gainesville, FL 32611, USA. Electronic address: hjiang@bme.ufl.edu.
authors.forename: Huabei
authors.initials: H
authors.lastname: Jiang
mesh_terms: D000818:Animals; D004569:Electroencephalography / Q000295:instrumentation*; D004827:Epilepsy / Q000503:physiopathology*; D006439:Hemodynamics; D061088:Photoacoustic Techniques / Q000295:instrumentation*; D017207:Rats, Sprague-Dawley
keywords: EEG; Neurovascular coupling; Pentylenetetrazol; Photoacoustic