title: Transferrin conjugated poly (γ-glutamic acid-maleimide-co-L-lactide)-1,2-dipalmitoylsn-glycero-3-phosphoethanolamine copolymer nanoparticles for targeting drug delivery.
abstract: Targeted drug delivery strategies have shown great potential in solving some problems of chemotherapy, such as non-selectivity and severe side effects, thus enhancing the anti-tumor efficiency of chemotherapeutic agents. In this work, we have prepared a novel nanoparticle consisted of amphiphilic poly(γ-glutamic acid-maleimide-co-L-lactide)-1,2-dipalmitoylsn-glycero-3-phosphoethanolamine (γ-PGA-MAL-PLA-DPPE) copolymer decorated with transferrin (Tf), which can specifically deliver anti-cancer drug paclitaxel (PTX) to the tumor cells for targeting chemotherapy. These nanoparticles (NPs) have preferable particle size, high encapsulation efficiency and a pH-dependent release profile. As expected, The Tf modification mediate specific targeting to nasopharyngeal carcinoma (C666-1) cells and human cervical carcinoma (Hela) cells with the transferrin receptor (TfR) overexpressed and enhance cellular uptake of the NPs, as demonstrated by flow cytometry and confocal microscopy assays. In vitro cytotoxicity studies reveal that the NPs have excellent biocompatibility, and the presence of Tf enhance the activity of PTX to the targeted cells. All these results prove that Tf modified γ-PGA-MAL-PLA-DPPE NPs could facilitate the tumor-specific therapy. Therefore, such a targeting drug delivery system provides significant advances toward cancer therapy.
authors.affiliation: National Center for Nanoscience and Technology, Laboratory of Nanobiomedicine and Nanosafety, Division of Nanomedicine and Nanobiology, No. 11 Beiyitiao, Zhongguancun, Beijing 100190, China.
authors.forename: Caiyan
authors.initials: C
authors.lastname: Zhao
authors.affiliation: National Center for Nanoscience and Technology, Laboratory of Nanobiomedicine and Nanosafety, Division of Nanomedicine and Nanobiology, No. 11 Beiyitiao, Zhongguancun, Beijing 100190, China; School of Basic Medical Sciences, Jiamusi University, Jiamusi 154007, China; The First Affiliated Hospital of Jiamusi University, Jiamusi 154002, China.
authors.forename: Xiaoguang
authors.initials: X
authors.lastname: Liu
authors.affiliation: School of Basic Medical Sciences, Jiamusi University, Jiamusi 154007, China; The First Affiliated Hospital of Jiamusi University, Jiamusi 154002, China. Electronic address: liujunxing0982@163.com.
authors.forename: Junxing
authors.initials: J
authors.lastname: Liu
authors.affiliation: School of Basic Medical Sciences, Jiamusi University, Jiamusi 154007, China; The First Affiliated Hospital of Jiamusi University, Jiamusi 154002, China.
authors.forename: Zhiwei
authors.initials: Z
authors.lastname: Yang
authors.affiliation: School of Basic Medical Sciences, Jiamusi University, Jiamusi 154007, China; The First Affiliated Hospital of Jiamusi University, Jiamusi 154002, China.
authors.forename: Xianghui
authors.initials: X
authors.lastname: Rong
authors.affiliation: School of Basic Medical Sciences, Jiamusi University, Jiamusi 154007, China; The First Affiliated Hospital of Jiamusi University, Jiamusi 154002, China. Electronic address: xjl1362@sina.com.
authors.forename: Mingjun
authors.initials: M
authors.lastname: Li
authors.affiliation: National Center for Nanoscience and Technology, Laboratory of Nanobiomedicine and Nanosafety, Division of Nanomedicine and Nanobiology, No. 11 Beiyitiao, Zhongguancun, Beijing 100190, China. Electronic address: liangxj@nanoctr.cn.
authors.forename: Xingjie
authors.initials: X
authors.lastname: Liang
authors.affiliation: National Center for Nanoscience and Technology, Laboratory of Nanobiomedicine and Nanosafety, Division of Nanomedicine and Nanobiology, No. 11 Beiyitiao, Zhongguancun, Beijing 100190, China. Electronic address: wuy@nanoctr.cn.
authors.forename: Yan
authors.initials: Y
authors.lastname: Wu
mesh_terms: D000970:Antineoplastic Agents / Q000008:administration & dosage / Q000737:chemistry; D045744:Cell Line, Tumor; D004337:Drug Carriers / Q000737:chemistry; D016503:Drug Delivery Systems / Q000379:methods*; D006801:Humans; D053758:Nanoparticles / Q000737:chemistry*; D017239:Paclitaxel / Q000008:administration & dosage / Q000737:chemistry; D010714:Phosphatidylethanolamines; D011099:Polyglutamic Acid / Q000031:analogs & derivatives* / Q000737:chemistry; D011108:Polymers / Q000737:chemistry*; D014168:Transferrin / Q000737:chemistry*
chemical_list: D000970:Antineoplastic Agents; D004337:Drug Carriers; D010714:Phosphatidylethanolamines; D011108:Polymers; D014168:Transferrin; C511775:poly(gamma-glutamic acid); D011099:Polyglutamic Acid; D017239:Paclitaxel
keywords: 1,2-Dipalmitoylsn-glycero-3-phosphoethanolamine; Amphiphilic copolymer; Paclitaxel; Poly γ-glutamic acid; Targeting delivery system; Transferrin