title: MutSα and MutLα immunoexpression analysis in diagnostic grading of oral epithelial dysplasia and squamous cell carcinoma.
abstract: OBJECTIVE
  This study explored the expression of DNA mismatch repair (MMR) proteins in a range of oral biopsies. We further evaluated the significance of MMR protein expression combined with basic demographic data in differentiating grades of oral epithelial dysplasia (OED) and oral squamous cell carcinoma (OSCC).
  
  
  METHODS
  Immunohistochemical expression of MutSα (hMLH1 and hPMS2) and MutLα (hMSH2 and hMSH6) were compared in 98 formalin-fixed paraffin embedded oral biopsies: 21 normal, 24 mild-dysplasia (MD), 8 moderate-to-severe-dysplasia (SD), and 45 OSCC.
  
  
  RESULTS
  Expression of hMLH1, hPMS2, and hMSH2 was reduced in MD, SD, and OSCC compared with the normal. Reduced hMSH2 immunoreactivity discriminated poorly differentiated OSCC from well-differentiated OSCC. The diagnostic model correctly classified 71.4% of cases and revealed that hPMS2-negative biopsies were more likely to be cancerous (odds ratio [OR], 0.11; 95% confidence interval [CI], 0.000-0.813; P = .040).
  
  
  CONCLUSIONS
  The results suggested a diagnostic role for MMR proteins in OED and OSCC.
authors.affiliation: The University of Queensland, UQ Centre for Clinical Research, Herston, QLD 4029, Australia; The University of Queensland, School of Dentistry, Brisbane, QLD 4000, Australia.
authors.forename: M
authors.initials: M
authors.lastname: Jessri
authors.affiliation: The University of Queensland, UQ Centre for Clinical Research, Herston, QLD 4029, Australia.
authors.forename: A J
authors.initials: AJ
authors.lastname: Dalley
authors.affiliation: The University of Queensland, UQ Centre for Clinical Research, Herston, QLD 4029, Australia; The University of Queensland, School of Dentistry, Brisbane, QLD 4000, Australia. Electronic address: camile@oralmedpath.com.au.
authors.forename: C S
authors.initials: CS
authors.lastname: Farah
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000378:metabolism; D000251:Adenosine Triphosphatases / Q000378:metabolism; D000293:Adolescent; D000328:Adult; D000368:Aged; D001706:Biopsy; D002278:Carcinoma in Situ / Q000378:metabolism* / Q000473:pathology; D002294:Carcinoma, Squamous Cell / Q000378:metabolism* / Q000473:pathology; D053843:DNA Mismatch Repair; D045643:DNA Repair Enzymes / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000378:metabolism; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D000070976:Mismatch Repair Endonuclease PMS2; D009062:Mouth Neoplasms / Q000378:metabolism* / Q000473:pathology; D000070957:MutL Protein Homolog 1; D000070956:MutL Proteins; D051719:MutS DNA Mismatch-Binding Protein / Q000378:metabolism*; D051718:MutS Homolog 2 Protein / Q000378:metabolism; D060787:Neoplasm Grading; D009687:Nuclear Proteins / Q000378:metabolism; D011230:Precancerous Conditions / Q000378:metabolism* / Q000473:pathology; D012737:Sex Factors
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D004268:DNA-Binding Proteins; C501486:MLH1 protein, human; C500929:MutLalpha protein, human; D009687:Nuclear Proteins; D000251:Adenosine Triphosphatases; C094934:PMS2 protein, human; C497172:MSH2 protein, human; D000070976:Mismatch Repair Endonuclease PMS2; D000070957:MutL Protein Homolog 1; D000070956:MutL Proteins; D051719:MutS DNA Mismatch-Binding Protein; D051718:MutS Homolog 2 Protein; D045643:DNA Repair Enzymes