title: Ionic liquid/ultrasound pretreatment and in situ enzymatic saccharification of bagasse using biocompatible cholinium ionic liquid.
abstract: Choline acetate (ChOAc), a cholinium ionic liquid (IL), showed almost the same bagasse pretreatment capability as 1-ethyl-3-methylimidazolium acetate (EmimOAc), a conventional imidazolium IL used for biomass pretreatment. Moreover, ChOAc showed less of an inhibitory effect on cellulase than EmimOAc. Thus, ChOAc was used for IL/ultrasound-assisted pretreatment and in situ enzymatic saccharification, where IL was not washed out from the pretreated bagasse but diluted with the addition of a buffer solution. When in situ saccharification was performed for 48h in the presence of 10% ChOAc, the cellulose and hemicellulose saccharification percentages were 80% and 72%, respectively. When ChOAc was increased to 20%, the saccharification percentages were 72% and 53%, respectively. However, the values were just 28% and 2%, respectively, in case of 20% EmimOAc. A glucose/xylose solution free from IL and ChOAc aqueous solution without these sugars could be recovered separately by electrodialysis of the hydrolysate of in situ saccharification.
authors.affiliation: Institute of Nature and Environmental Technology, Kanazawa University, Kakuma-machi, Kanazawa 920-1192, Japan.
authors.forename: Kazuaki
authors.initials: K
authors.lastname: Ninomiya
authors.affiliation: Faculty of Natural System, Institute of Science and Engineering, Kanazawa University, Kakuma-machi, Kanazawa 920-1192, Japan.
authors.forename: Asami
authors.initials: A
authors.lastname: Kohori
authors.affiliation: Faculty of Natural System, Institute of Science and Engineering, Kanazawa University, Kakuma-machi, Kanazawa 920-1192, Japan.
authors.forename: Mai
authors.initials: M
authors.lastname: Tatsumi
authors.affiliation: Faculty of Natural System, Institute of Science and Engineering, Kanazawa University, Kakuma-machi, Kanazawa 920-1192, Japan.
authors.forename: Koji
authors.initials: K
authors.lastname: Osawa
authors.affiliation: Faculty of Natural System, Institute of Science and Engineering, Kanazawa University, Kakuma-machi, Kanazawa 920-1192, Japan.
authors.forename: Takatsugu
authors.initials: T
authors.lastname: Endo
authors.affiliation: Faculty of Natural System, Institute of Science and Engineering, Kanazawa University, Kakuma-machi, Kanazawa 920-1192, Japan.
authors.forename: Ryohei
authors.initials: R
authors.lastname: Kakuchi
authors.affiliation: Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodaicho, Nada-ku, Kobe 657-8501, Japan.
authors.forename: Chiaki
authors.initials: C
authors.lastname: Ogino
authors.affiliation: Institute of Nature and Environmental Technology, Kanazawa University, Kakuma-machi, Kanazawa 920-1192, Japan.
authors.forename: Nobuaki
authors.initials: N
authors.lastname: Shimizu
authors.affiliation: Faculty of Natural System, Institute of Science and Engineering, Kanazawa University, Kakuma-machi, Kanazawa 920-1192, Japan. Electronic address: ktkenji@staff.kanazawa-u.ac.jp.
authors.forename: Kenji
authors.initials: K
authors.lastname: Takahashi
mesh_terms: D000085:Acetates / Q000737:chemistry*; D018533:Biomass; D001709:Biotechnology; D002480:Cellulase; D002482:Cellulose / Q000737:chemistry*; D002794:Choline / Q000737:chemistry*; D007093:Imidazoles; D052578:Ionic Liquids; D011134:Polysaccharides; D014465:Ultrasonics; D014961:X-Ray Diffraction
chemical_list: D000085:Acetates; D007093:Imidazoles; D052578:Ionic Liquids; D011134:Polysaccharides; C007916:hemicellulose; D002482:Cellulose; C027433:bagasse; D002480:Cellulase; D002794:Choline; C518739:1-ethyl-3-methylimidazolium
keywords: Cholinium; In situ saccharification; Ionic liquid; Lignocellulose; Pretreatment