title: Clinical epidemiology and molecular profiling of human bocavirus in faecal samples from children with diarrhoea in Guangzhou, China.
abstract: To understand the clinical epidemiology and molecular characteristics of human bocavirus (HBoV) infection in children with diarrhoea in Guangzhou, South China, we collected 1128 faecal specimens from children with diarrhoea from July 2010 to December 2012. HBoV and five other major enteric viruses were examined using real-time polymerase chain reaction. Human rotavirus (HRV) was the most prevalent pathogen, detected in 250 (22·2%) cases, followed by enteric adenovirus (EADV) in 76 (6·7%) cases, human astrovirus (HAstV) in 38 (3·4%) cases, HBoV in 17 (1·5%) cases, sapovirus (SaV) in 14 (1·2%) cases, and norovirus (NoV) in 9 (0·8%) cases. Co-infections were identified in 3·7% of the study population and 23·5% of HBoV-positive specimens. Phylogenetic analysis revealed 14 HBoV strains to be clustered into species HBoV1 with only minor variations among them. Overall, the detection of HBoV appears to partially contribute to the overall detection gap for enteric infections, single HBoV infection rarely results in severe clinical outcomes, and HBoV sequencing data appears to support conserved genomes across strains identified in this study.
authors.affiliation: Department of Medical Statistic and Epidemiology,School of Public Health, Sun Yat-sen University,Guangzhou,Guangdong,China.
authors.forename: D-M
authors.initials: DM
authors.lastname: Zhang
authors.affiliation: Department of Medical Statistic and Epidemiology,School of Public Health, Sun Yat-sen University,Guangzhou,Guangdong,China.
authors.forename: M-M
authors.initials: MM
authors.lastname: Ma
authors.affiliation: Key Laboratory for Tropical Disease Control,Ministry of Education,Guangzhou,Guangdong,China.
authors.forename: W-T
authors.initials: WT
authors.lastname: Wen
authors.affiliation: Key Laboratory for Tropical Disease Control,Ministry of Education,Guangzhou,Guangdong,China.
authors.forename: X
authors.initials: X
authors.lastname: Zhu
authors.affiliation: Key Laboratory for Tropical Disease Control,Ministry of Education,Guangzhou,Guangdong,China.
authors.forename: L
authors.initials: L
authors.lastname: Xu
authors.affiliation: Department of Medical Statistic and Epidemiology,School of Public Health, Sun Yat-sen University,Guangzhou,Guangdong,China.
authors.forename: Z-J
authors.initials: ZJ
authors.lastname: He
authors.affiliation: Department of Clinical Laboratory,Sun Yat-sen University Cancer Centre,Guangzhou,Guangdong,China.
authors.forename: X
authors.initials: X
authors.lastname: He
authors.affiliation: Key Laboratory for Tropical Disease Control,Ministry of Education,Guangzhou,Guangdong,China.
authors.forename: J-H
authors.initials: JH
authors.lastname: Wu
authors.affiliation: Key Laboratory for Tropical Disease Control,Ministry of Education,Guangzhou,Guangdong,China.
authors.forename: Y-W
authors.initials: YW
authors.lastname: Hu
authors.affiliation: Key Laboratory for Tropical Disease Control,Ministry of Education,Guangzhou,Guangdong,China.
authors.forename: Y
authors.initials: Y
authors.lastname: Zheng
authors.affiliation: Department of Medical Statistic and Epidemiology,School of Public Health, Sun Yat-sen University,Guangzhou,Guangdong,China.
authors.forename: Y
authors.initials: Y
authors.lastname: Deng
authors.affiliation: Key Laboratory for Tropical Disease Control,Ministry of Education,Guangzhou,Guangdong,China.
authors.forename: C-J
authors.initials: CJ
authors.lastname: Lin
authors.affiliation: Department of Medical Statistic and Epidemiology,School of Public Health, Sun Yat-sen University,Guangzhou,Guangdong,China.
authors.forename: J-H
authors.initials: JH
authors.lastname: Lu
authors.affiliation: Key Laboratory for Tropical Disease Control,Ministry of Education,Guangzhou,Guangdong,China.
authors.forename: M-F
authors.initials: MF
authors.lastname: Li
authors.affiliation: Key Laboratory for Tropical Disease Control,Ministry of Education,Guangzhou,Guangdong,China.
authors.forename: K-Y
authors.initials: KY
authors.lastname: Cao
mesh_terms: D000256:Adenoviridae / Q000235:genetics; D000258:Adenovirus Infections, Human / Q000453:epidemiology / Q000821:virology; D000293:Adolescent; D019350:Astroviridae Infections / Q000453:epidemiology / Q000821:virology; D017250:Caliciviridae Infections / Q000453:epidemiology / Q000821:virology; D002648:Child; D002675:Child, Preschool; D002681:China / Q000453:epidemiology; D060085:Coinfection / Q000453:epidemiology / Q000821:virology; D003430:Cross-Sectional Studies; D004279:DNA, Viral / Q000032:analysis*; D003967:Diarrhea / Q000453:epidemiology* / Q000821:virology; D005243:Feces / Q000821:virology; D005260:Female; D005759:Gastroenteritis / Q000453:epidemiology* / Q000821:virology; D056661:Human bocavirus / Q000235:genetics*; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D001257:Mamastrovirus / Q000235:genetics; D029322:Norovirus / Q000235:genetics; D010322:Parvoviridae Infections / Q000453:epidemiology* / Q000821:virology; D012367:RNA, Viral / Q000032:analysis*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012401:Rotavirus / Q000235:genetics; D012400:Rotavirus Infections / Q000453:epidemiology / Q000821:virology; D029341:Sapovirus / Q000235:genetics
chemical_list: D004279:DNA, Viral; D012367:RNA, Viral
keywords: Children; diarrhoea; human bocavirus; phylogenetic analysis