title: Identification of novel steroidogenic factor 1 (SF-1)-target genes and components of the SF-1 nuclear complex.
abstract: Steroidogenic factor 1 (SF-1) is a master regulator of adrenal and reproductive development and function. Although SF-1 was identified as a transcriptional regulator for steroid metabolic enzymes, it has been shown that SF-1 also regulates other genes that are involved in various cellular processes. Previously, we showed that introduction of SF-1 into mesenchymal stem cells resulted in the differentiation of these cells to the steroidogenic lineage. By using this method of differentiation, we performed comprehensive analyses to identify the novel SF-1-target genes and components of the SF-1 nuclear complex. Genome-wide analyses with promoter tiling array and DNA microarray identified 10 genes as novel SF-1-target genes including glutathione S-transferase A family, 5-aminolevulinic acid synthase 1 and ferredoxin reductase. Using SF-1 immuno-affinity chromatography of nuclear proteins followed by MS/MS analysis, we identified 24 proteins including CCAAT/enhancer-binding protein β as components of SF-1 nuclear complex. In this review, we will describe novel roles of the newly identified genes for steroidogenesis.
authors.affiliation: Department of Biochemistry, Faculty of Medical Sciences, University of Fukui, Fukui 910-1193, Japan; Translational Research Center, Organization for Life Science Advancement Programs, University of Fukui, Fukui 910-1193, Japan. Electronic address: mizutani@u-fukui.ac.jp.
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Mizutani
authors.affiliation: Department of Biochemistry, Faculty of Medical Sciences, University of Fukui, Fukui 910-1193, Japan; Translational Research Center, Organization for Life Science Advancement Programs, University of Fukui, Fukui 910-1193, Japan.
authors.forename: Shinya
authors.initials: S
authors.lastname: Kawabe
authors.affiliation: Department of Biochemistry, Faculty of Medical Sciences, University of Fukui, Fukui 910-1193, Japan.
authors.forename: Shin
authors.initials: S
authors.lastname: Ishikane
authors.affiliation: Department of Biochemistry, Faculty of Medical Sciences, University of Fukui, Fukui 910-1193, Japan; Translational Research Center, Organization for Life Science Advancement Programs, University of Fukui, Fukui 910-1193, Japan.
authors.forename: Yoshitaka
authors.initials: Y
authors.lastname: Imamichi
authors.affiliation: National Research Institute for Child Health and Development, Tokyo 157-8535, Japan.
authors.forename: Akihiro
authors.initials: A
authors.lastname: Umezawa
authors.affiliation: Department of Biochemistry, Faculty of Medical Sciences, University of Fukui, Fukui 910-1193, Japan; Translational Research Center, Organization for Life Science Advancement Programs, University of Fukui, Fukui 910-1193, Japan.
authors.forename: Kaoru
authors.initials: K
authors.lastname: Miyamoto
mesh_terms: D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism*; D056726:Genetic Association Studies; D006801:Humans; D008954:Models, Biological; D046912:Multiprotein Complexes / Q000378:metabolism*; D054339:Steroidogenic Factor 1 / Q000378:metabolism*
chemical_list: D046912:Multiprotein Complexes; D054339:Steroidogenic Factor 1
keywords: 5-aminolevulinic acid synthase 1; CCAAT/enhancer-binding protein β; Ferredoxin reductase; Glutathione S-transferase A family; Steroidogenesis; Transcriptional regulation