title: Development of a replicative plasmid for gene expression in Mycoplasma bovis.
abstract: Mycoplasma bovis (M. bovis) is a pathogen related to a variety of disease syndromes that result in significant economic losses in the cattle industry. Here, a stable replicative plasmid system is developed for use in M. bovis, utilizing an origin of replication (oriC) region. Additionally, the heterologous protein β-galactosidase and a FLAG tag-fused endogenous protein were successfully expressed by this plasmid system. These findings provide evidence that this oriC-based vector is applicable for the study of M. bovis.
authors.affiliation: Key Laboratory of Zoonosis of Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing, China.
authors.forename: Jiahe
authors.initials: J
authors.lastname: Li
authors.affiliation: State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing, China.
authors.forename: Jixiang
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Zoonosis of Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing, China.
authors.forename: Ning
authors.initials: N
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Zoonosis of Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing, China.
authors.forename: Yuewei
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Zoonosis of Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing, China. Electronic address: wuwenxue@cau.edu.cn.
authors.forename: Wenxue
authors.initials: W
authors.lastname: Wu
authors.affiliation: Chinese Academy of Agricultural Sciences, Beijing, China. Electronic address: lijinxiang@caas.cn.
authors.forename: Jinxiang
authors.initials: J
authors.lastname: Li
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D004261:DNA Replication; D015870:Gene Expression; D045682:Mycoplasma bovis / Q000235:genetics* / Q000378:metabolism; D010957:Plasmids / Q000235:genetics* / Q000378:metabolism; D018741:Replication Origin; D014169:Transformation, Bacterial; D001616:beta-Galactosidase / Q000235:genetics / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D001616:beta-Galactosidase
keywords: Gene expression; Mycoplasma bovis; Plasmids; Transformation; oriC