title: HPLC analysis of blood-brain barrier penetration of 4-fluorodeprenyl.
abstract: Validated HPLC analysis was developed in order to monitor the level of 4-fluorodeprenyl in rats. Male Wistar rats were intraperitoneally treated with 30 mg/kg of (-)-4-fluorodeprenyl. The rats were sacrificed after 5, 15, 30 and 60 min of treatment, and various tissues were isolated, such as serum, brain, CSF, liver, testis and lacrimal gland. Perchloric acid was given to aliquots, which were then homogenized, centrifuged and the supernatants were taken. The 4-fluorodeprenyl content was determined using reversed-phase HPLC, based on the comparison of the calibration line of the spiked samples. The level of 4-fluorodeprenyl was between 0.5 and 24 μg/g, showing maximum concentration in the brain and the liver after 5 min following its administration and in serum, CSF, testis, eyes and lacrimal gland after 15 min following its administration, while a relatively high concentration was found in the liver and the lacrimal gland.
authors.affiliation: Department of Pharmacology and Pharmacotherapy, Semmelweis University, Nagyvárad tér 4, Budapest, Hungary. Electronic address: postenyi.zita@gmail.com.
authors.forename: Zita
authors.initials: Z
authors.lastname: Pöstényi
authors.affiliation: Department of Pharmacodynamics, Semmelweis University, Nagyvárad tér 4, Budapest, Hungary. Electronic address: drtekes@gmail.com.
authors.forename: Kornélia
authors.initials: K
authors.lastname: Tekes
authors.affiliation: Department of Ophthalmology, University of Szeged, Szeged, Hungary. Electronic address: tme@tmedit.hu.
authors.forename: Edit
authors.initials: E
authors.lastname: Tóth-Molnár
authors.affiliation: Department of Pharmacology and Pharmacotherapy, Semmelweis University, Nagyvárad tér 4, Budapest, Hungary. Electronic address: drkalasz@gmail.com.
authors.forename: Huba
authors.initials: H
authors.lastname: Kalász
mesh_terms: D000818:Animals; D001692:Biological Transport / Q000502:physiology; D001812:Blood-Brain Barrier / Q000187:drug effects / Q000378:metabolism*; D001921:Brain / Q000187:drug effects / Q000378:metabolism*; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D012642:Selegiline / Q000031:analogs & derivatives* / Q000032:analysis / Q000378:metabolism / Q000494:pharmacology
chemical_list: C064772:4-fluorodeprenyl; D012642:Selegiline
keywords: (−)-4-Fluorodeprenyl; Blood–Brain barrier (BBB); HPLC; Lacrimal gland; Rats