title: Effects of nitrogen source availability and bioreactor operating strategies on lutein production with Scenedesmus obliquus FSP-3.
abstract: In this study, the effects of the type and concentration of nitrogen sources on the cell growth and lutein content of an isolated microalga Scenedesmus obliquus FSP-3 were investigated. With batch culture, the highest lutein content (4.61 mg/g) and lutein productivity (4.35 mg/L/day) were obtained when using 8.0 mM calcium nitrate as the nitrogen source. With this best nitrogen source condition, the microalgae cultivation was performed using two bioreactor strategies (namely, semi-continuous and two-stage operations) to further enhance the lutein content and productivity. Using semi-continuous operation with a 10% medium replacement ratio could obtain the highest biomass productivity (1304.8 mg/L/day) and lutein productivity (6.01 mg/L/day). This performance is better than most related studies.
authors.affiliation: Organization of Advanced Science and Technology, Kobe University, Kobe 657-8501, Japan.
authors.forename: Shih-Hsin
authors.initials: SH
authors.lastname: Ho
authors.affiliation: College of Biological Science and Engineering, Fuzhou University, Fuzhou 350108, PR China.
authors.forename: Youping
authors.initials: Y
authors.lastname: Xie
authors.affiliation: Department of Chemical Engineering, National Cheng Kung University, Tainan 701, Taiwan.
authors.forename: Ming-Chang
authors.initials: MC
authors.lastname: Chan
authors.affiliation: Center for Bioscience and Biotechnology, National Cheng Kung University, Tainan 701, Taiwan.
authors.forename: Chen-Chun
authors.initials: CC
authors.lastname: Liu
authors.affiliation: Center for Bioscience and Biotechnology, National Cheng Kung University, Tainan 701, Taiwan.
authors.forename: Chun-Yen
authors.initials: CY
authors.lastname: Chen
authors.affiliation: Department of Chemical Engineering, National Taiwan University of Science and Technology, Taipei, Taiwan.
authors.forename: Duu-Jong
authors.initials: DJ
authors.lastname: Lee
authors.affiliation: Agricultural Biotechnology Center, National Chung Hsing University, Taichung, Taiwan.
authors.forename: Chieh-Chen
authors.initials: CC
authors.lastname: Huang
authors.affiliation: Department of Chemical Engineering, National Cheng Kung University, Tainan 701, Taiwan; Center for Bioscience and Biotechnology, National Cheng Kung University, Tainan 701, Taiwan; Research Center for Energy Technology and Strategy Center, National Cheng Kung University, Tainan 701, Taiwan. Electronic address: changjs@mail.ncku.edu.tw.
authors.forename: Jo-Shu
authors.initials: JS
authors.lastname: Chang
mesh_terms: D061249:Batch Cell Culture Techniques; D018533:Biomass; D019149:Bioreactors / Q000382:microbiology*; D008027:Light; D014975:Lutein / Q000096:biosynthesis*; D009584:Nitrogen / Q000494:pharmacology*; D044444:Scenedesmus / Q000166:cytology / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism* / Q000528:radiation effects; D013997:Time Factors
chemical_list: D009584:Nitrogen; D014975:Lutein
keywords: Bioreactor strategy; Light intensity shift; Lutein; Microalgae; Scenedesmus obliquus