title: Whole transverse section and specific-tissue analysis of secondary metabolites in seven different grades of root of Paeonia lactiflora using laser microdissection and liquid chromatography-quadrupole/time of flight-mass spectrometry.
abstract: The root of Paeonia lactiflora Pall. is widely used in the pharmaceutical, food and cosmetic industries. For these purposes, roots are graded according to diameter, with larger roots considered to be of better quality. To assess the inherent quality of different grades and of different tissues in roots of P. lactiflora, here laser microdissection coupled with UPLC-Q/TOF-MS was applied. The results show the quantity of pharmaceutically important components decreased with increase in root diameter from 0.3cm to 0.7cm. Above 0.7cm of diameter, quantity and diversity of these components increased proportionally with increase in root diameter. The tissue-specific study indicated that the high content of paeoniflorin and albiflorin are mainly distributed in the cork and cortex. According to the results of this study, the roots of P. lactiflora greater than 1.7cm in diameter are of better quality medicinal use than smaller, and the commercial grades chose was best cutoff points. The fine roots and the outer bank of roots, which besides the commercial grades, contain such significant amounts of chemical components too. This study provides a new and practical method for evaluating the different grades of P. lactiflora.
authors.affiliation: Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100193, People's Republic of China.
authors.forename: Qiuling
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Chinese Academy of Medical Sciences, Peking Union Medical College, Hong Kong Baptist University, Kowloon Tong, Kowloon 999077, Hong Kong, China.
authors.forename: Zhitao
authors.initials: Z
authors.lastname: Liang
authors.affiliation: Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100193, People's Republic of China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Peng
authors.affiliation: School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, People's Republic of China.
authors.forename: Jun Ling
authors.initials: JL
authors.lastname: Hou
authors.affiliation: School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, People's Republic of China.
authors.forename: Sheng Li
authors.initials: SL
authors.lastname: Wei
authors.affiliation: Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100193, People's Republic of China; Chinese Academy of Medical Sciences, Peking Union Medical College, Hong Kong Baptist University, Kowloon Tong, Kowloon 999077, Hong Kong, China. Electronic address: zzzhao@hkbu.edu.hk.
authors.forename: Zhong Zhen
authors.initials: ZZ
authors.lastname: Zhao
authors.affiliation: Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100193, People's Republic of China; School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, People's Republic of China. Electronic address: wqwang@implad.ac.cn.
authors.forename: Wen Quan
authors.initials: WQ
authors.lastname: Wang
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D007834:Lasers; D057230:Limit of Detection; D013058:Mass Spectrometry; D029596:Paeonia / Q000378:metabolism*; D018517:Plant Roots / Q000378:metabolism*; D015203:Reproducibility of Results
keywords: Grade; Laser microdissection; Paeonia lactiflora Pall.; UPLC-Q/TOF–MS