title: Genotoxic effects of CdS quantum dots and Ag2S nanoparticles in fish cell lines (RTG-2).
abstract: The increasing use of nanotechnologies will lead to significant releases of engineered nanoparticles into the aquatic environment, where their impact is still poorly characterized. In the present paper, the genotoxic and cytotoxic properties of CdS quantum dots (QDs) and silver sulphide (Ag2S) coated with methyl polyethylene glycol (M-PEG) were investigated in a rainbow trout cell line (RTG-2). The results showed that CdS QDs were highly cytotoxic at high concentrations (10 and 50μg/ml), and exhibited a concentration-dependent genotoxicity in the sub-toxic range (0.01-1μg/ml) after 24h exposure. Ag2S showed neither genotoxic nor cytotoxic effects.
authors.affiliation: Department of Biology, University of Padua, Padua, Italy.
authors.forename: Marco
authors.initials: M
authors.lastname: Munari
authors.affiliation: Department of Biological and Environmental Sciences, University of Gothenburg, Göteborg, Sweden.
authors.forename: Joachim
authors.initials: J
authors.lastname: Sturve
authors.affiliation: Department of Clinical and Experimental Medicine, University of Pisa, Italy. Electronic address: giada@biomed.unipi.it.
authors.forename: Giada
authors.initials: G
authors.lastname: Frenzilli
authors.affiliation: Cefas Weymouth Laboratory, Barrack Road, The Nothe, Weymouth, Dorset DT4 8UB, UK.
authors.forename: Matthew B
authors.initials: MB
authors.lastname: Sanders
authors.affiliation: Department of Environmental Science, Ca' Foscari University, Dorsoduro 2137, I-30123 Venice, Italy.
authors.forename: Andrea
authors.initials: A
authors.lastname: Brunelli
authors.affiliation: Department of Environmental Science, Ca' Foscari University, Dorsoduro 2137, I-30123 Venice, Italy.
authors.forename: Antonio
authors.initials: A
authors.lastname: Marcomini
authors.affiliation: Department of Clinical and Experimental Medicine, University of Pisa, Italy.
authors.forename: Marco
authors.initials: M
authors.lastname: Nigro
authors.affiliation: Cefas Weymouth Laboratory, Barrack Road, The Nothe, Weymouth, Dorset DT4 8UB, UK.
authors.forename: Brett P
authors.initials: BP
authors.lastname: Lyons
mesh_terms: D000818:Animals; D019187:Cadmium Compounds / Q000138:chemical synthesis / Q000737:chemistry / Q000633:toxicity*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D004249:DNA Damage; D004305:Dose-Response Relationship, Drug; D006066:Gonads / Q000187:drug effects; D053768:Metal Nanoparticles / Q000737:chemistry / Q000633:toxicity; D023421:Models, Animal; D017686:Oncorhynchus mykiss; D011092:Polyethylene Glycols / Q000737:chemistry; D018030:Silver Compounds / Q000138:chemical synthesis / Q000633:toxicity*; D013440:Sulfides / Q000138:chemical synthesis / Q000737:chemistry / Q000633:toxicity*
chemical_list: D019187:Cadmium Compounds; D018030:Silver Compounds; D013440:Sulfides; C034939:cadmium sulfide; D011092:Polyethylene Glycols; C013251:silver sulfide
keywords: Cadmium; DNA damage; Fish cell culture; Nanoparticles; Quantum dots; Silver