title: In vitro monitoring of oxidative processes with self-aggregating gold nanoparticles using all-optical photoacoustic spectroscopy.
abstract: In this work, the assembly of gold nanoparticles of (AuNPs) is used to detect the presence of the biomolecule glutathione (GSH) using a novel technique called "all-optical photoacoustic spectroscopy" (AOPAS). The AOPAS technique coupled with AuNPs forms the basis of a biosensing technique capable of probing the dynamic evolution of nano-bio interfaces within a microscopic volume. Dynamic Light Scattering (DLS) and ultraviolet-visible (UV-vis) spectra were measured to describe the kinetics governing the interparticle interactions by monitoring the AuNPs assembly and evolution of the surface plasmon resonance (SPR) band. A comparison of the same dynamic evolution of AuNPs assembly was performed using the AOPAS technique to confirm the validity of this method. The fundamental study is complemented by a demonstration of the performance of this biosensing technique in the presence of cell culture medium containing fetal bovine serum (FBS), which forms a protein corona on the surface of the AuNPs. This work demonstrates that the in vitro monitoring capabilities of the AOPAS provides sensitive measurement at the microscopic level and low nanoparticle concentrations without the artifacts limiting the use of conventional biosensing methods, such as fluorescent indicators. The AOPAS technique not only provides a facile approach for in vitro biosensing, but also shed a light on the real-time detection of thiol containing oxidative stress biomarkers in live systems using AuNPs.
authors.affiliation: Department of Physics and Astronomy, University of Texas at San Antonio, One UTSA Circle, San Antonio, TX 78249, USA. Electronic address: zannatul.yasmin@gmail.com.
authors.forename: Zannatul
authors.initials: Z
authors.lastname: Yasmin
authors.affiliation: Department of Physics and Astronomy, University of Texas at San Antonio, One UTSA Circle, San Antonio, TX 78249, USA. Electronic address: ed11kh@yahoo.com.
authors.forename: Edward
authors.initials: E
authors.lastname: Khachatryan
authors.affiliation: Department of Radiology, University of Texas Health Science Center at San Antonio, San Antonio, TX 78229, USA; Department of Oncologic Sciences and Mitchell Cancer Cell Institute, University of Alabama, Mobile, AL 36688, USA. Electronic address: leey4@uthscsa.edu.
authors.forename: Yuan-Hao
authors.initials: YH
authors.lastname: Lee
authors.affiliation: Department of Ophthalmology, University of Texas Health Science Center at San Antonio, San Antonio, TX 78229, USA. Electronic address: Saher.maswadi@utsa.edu.
authors.forename: Saher
authors.initials: S
authors.lastname: Maswadi
authors.affiliation: Department of Physics and Astronomy, University of Texas at San Antonio, One UTSA Circle, San Antonio, TX 78249, USA; Department of Ophthalmology, University of Texas Health Science Center at San Antonio, San Antonio, TX 78229, USA; Center for Biomedical Neuroscience, University of Texas Health Science Center at San Antonio, San Antonio, TX 78229, USA. Electronic address: glickman@uthscsa.edu.
authors.forename: Randolph
authors.initials: R
authors.lastname: Glickman
authors.affiliation: Department of Physics and Astronomy, University of Texas at San Antonio, One UTSA Circle, San Antonio, TX 78249, USA. Electronic address: Kelly.Nash@utsa.edu.
authors.forename: Kelly L
authors.initials: KL
authors.lastname: Nash
mesh_terms: D004867:Equipment Design; D019544:Equipment Failure Analysis; D005978:Glutathione / Q000032:analysis* / Q000737:chemistry*; D006046:Gold / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry*; D008832:Microchemistry / Q000295:instrumentation*; D010084:Oxidation-Reduction; D061088:Photoacoustic Techniques / Q000295:instrumentation*; D013057:Spectrum Analysis / Q000295:instrumentation*
chemical_list: D006046:Gold; D005978:Glutathione
keywords: Biosensors; Gold nanoparticles; In situ sensing; Nano-bio interface; Photoacoustic spectroscopy; Protein corona