title: Hydrogen production by Rhodobacter sphaeroides DSM 158 under intense irradiation.
abstract: To identify optimal hydrogen production conditions using growing cultures of Rhodobacter sphaeroides DSM 158 the effects of varying the reactor's volumetric power input (0.01-1.4kWm(-3)) and irradiation intensity (5-2500Wm(-2)) were investigated in batch and continuous production modes. Irradiation intensity had a greater effect on hydrogen production than volumetric power input. Hydrogen production and photofermentative biomass formation were maximized by irradiation at 2250Wm(-2) with a volumetric power input of 0.55kWm(-3). The bacterial dry weight (2.64gL(-1)) and rate of hydrogen production (195mLL(-1)h(-1)) achieved under these conditions were greater than any that have previously been reported for batch-mode hydrogen production by R. sphaeroides. Continuous mode experiments (D=0.1h(-1)) yielded a bacterial dry weight, hydrogen production rate, productivity and hydrogen yield of 2.35±0.18gL(-1), 165±6.2mLL(-1)h(-1), 3.96LL(-1)d(-1) and 36.6%, respectively.
authors.affiliation: Institute of Food Technology and Bioprocess Engineering, TU Dresden, Bergstraße 120, 01069 Dresden, Germany. Electronic address: Felix.Krujatz@tu-dresden.de.
authors.forename: Felix
authors.initials: F
authors.lastname: Krujatz
authors.affiliation: Institute of Food Technology and Bioprocess Engineering, TU Dresden, Bergstraße 120, 01069 Dresden, Germany. Electronic address: paulhaertel@gmx.de.
authors.forename: Paul
authors.initials: P
authors.lastname: Härtel
authors.affiliation: Institute of Food Technology and Bioprocess Engineering, TU Dresden, Bergstraße 120, 01069 Dresden, Germany. Electronic address: Karsten.Helbig@tu-dresden.de.
authors.forename: Karsten
authors.initials: K
authors.lastname: Helbig
authors.affiliation: Institute of Physical Chemistry, TU Dresden, Eisenstuckstraße 5, 01069 Dresden, Germany. Electronic address: Nora.Haufe@tu-dresden.de.
authors.forename: Nora
authors.initials: N
authors.lastname: Haufe
authors.affiliation: Institute of Food Technology and Bioprocess Engineering, TU Dresden, Bergstraße 120, 01069 Dresden, Germany. Electronic address: Simone.Thierfelder@tu-dresden.de.
authors.forename: Simone
authors.initials: S
authors.lastname: Thierfelder
authors.affiliation: Institute of Food Technology and Bioprocess Engineering, TU Dresden, Bergstraße 120, 01069 Dresden, Germany. Electronic address: Thomas.Bley@tu-dresden.de.
authors.forename: Thomas
authors.initials: T
authors.lastname: Bley
authors.affiliation: Institute of Food Technology and Bioprocess Engineering, TU Dresden, Bergstraße 120, 01069 Dresden, Germany. Electronic address: Jost.Weber@tu-dresden.de.
authors.forename: Jost
authors.initials: J
authors.lastname: Weber
mesh_terms: D018533:Biomass; D006859:Hydrogen / Q000378:metabolism*; D058433:Photobioreactors / Q000382:microbiology*; D012242:Rhodobacter sphaeroides / Q000378:metabolism*
chemical_list: D006859:Hydrogen
keywords: Batch; Continuous; Hydrogen; Intense irradiation; Photofermentation