title: Affinity purification and characterisation of zinc chelating peptides from rapeseed protein hydrolysates: possible contribution of characteristic amino acid residues.
abstract: Zinc is an essential trace element for human growth and development. In this work, zinc-chelating peptides from rapeseed protein hydrolysates produced with alcalase were investigated by affinity chromatography with immobilized zinc and Sephadex G-25 gel filtration. Four small peptides, namely, Ala-Arg, Asn-Ser-Met (NSM), Gly-Lys-Arg, and Glu-Pro-Ser-His, were obtained and identified by reversed-phase high-performance liquid chromatography and electrospray ionization mass spectrometry. The zinc-chelating ability of the four peptides was further validated by inductively coupled plasma atomic emission spectrometry (ICP-AES). NSM was found to exhibit the highest zinc-chelating rate, which was better than that of reduced glutathione. We speculated that the Asn residue at the amino-terminus might facilitate this zinc-chelating ability. Therefore, utilizing small peptides from rapeseed protein as novel carriers for zinc supplement was feasible.
authors.affiliation: Institute of Agro-food Science and Technology, Anhui Academy of Agricultural Sciences, Hefei 230031, China. Electronic address: ningxie512@gmail.com.
authors.forename: Ningning
authors.initials: N
authors.lastname: Xie
authors.affiliation: Institute of Agro-food Science and Technology, Anhui Academy of Agricultural Sciences, Hefei 230031, China.
authors.forename: Jingjing
authors.initials: J
authors.lastname: Huang
authors.affiliation: College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China; Key Laboratory of Functional Dairy, Ministry of Education, Beijing 100083, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Li
authors.affiliation: Institute of Agro-food Science and Technology, Anhui Academy of Agricultural Sciences, Hefei 230031, China.
authors.forename: Jianghua
authors.initials: J
authors.lastname: Cheng
authors.affiliation: Institute of Agro-food Science and Technology, Anhui Academy of Agricultural Sciences, Hefei 230031, China.
authors.forename: Zhuochen
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Institute of Agro-food Science and Technology, Anhui Academy of Agricultural Sciences, Hefei 230031, China.
authors.forename: Junfeng
authors.initials: J
authors.lastname: Yin
authors.affiliation: Institute of Agro-food Science and Technology, Anhui Academy of Agricultural Sciences, Hefei 230031, China.
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Yan
mesh_terms: D000595:Amino Acid Sequence; D000596:Amino Acids / Q000378:metabolism; D029741:Brassica rapa / Q000737:chemistry*; D002614:Chelating Agents / Q000302:isolation & purification* / Q000378:metabolism; D002850:Chromatography, Gel; D004337:Drug Carriers; D006801:Humans; D008969:Molecular Sequence Data; D010455:Peptides / Q000302:isolation & purification* / Q000378:metabolism; D011492:Protein Hydrolysates / Q000032:analysis*; D015032:Zinc / Q000378:metabolism*
chemical_list: D000596:Amino Acids; D002614:Chelating Agents; D004337:Drug Carriers; D010455:Peptides; D011492:Protein Hydrolysates; D015032:Zinc
keywords: Alcalase; Chelating peptides; Immobilized metal affinity chromatography; Rapeseed protein hydrolysates; Zinc