title: Preventive effect ofLactobacillus fermentumLee on activated carbon-induced constipation in mice.
abstract: The aim of this study was to investigate the effects ofLactobacillus fermentumLee (LF-Lee) on activated carbon-induced constipation in ICR mice. ICR mice were orally administered lactic acid bacteria for nine days. Body weight, dietary and water intake, defecation status, gastrointestinal (GI) transit and defecation time, as well as levels of motilin (MTL), gastrin (Gas), endothelin (ET), somatostatin (SS), acetylcholinesterase (AChE), substance P (SP) and vasoactive intestinal peptide (VIP) in serum were measured to evaluate the preventive effects of LF-Lee on constipation. Bisacodyl, a laxative drug, was administered as a positive control. The time taken until the first defecation of a black stool for normal, control, bisacodyl- (100 mg/kg, oral administration),Lactobacillus bulgaricus(LB)-, LF-Lee low dose (L)- and LF-Lee high dose (H)-treated mice was 90, 218, 117, 180, 161 and 151 min, respectively. Following the consumption of LB, LF-Lee (L) or LF-Lee (H), or the oral administration of bisacodyl, the GI transit was reduced to 55.2, 65.8, 73.1 and 94.6%, respectively, of the transit in normal mice. The serum levels of MTL, Gas, ET, AChE, SP and VIP were significantly increased and those of SS were reduced in the mice treated with LF-Lee compared with those in the untreated control mice (P<0.05). These results demonstrate that lactic acid bacteria have preventive effects on constipation in mice and that LF-Lee has superior functional activity.
authors.affiliation: Department of Biological and Chemical Engineering, Chongqing University of Education, Chongqing 400067, P.R. China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Qian
authors.affiliation: College of Food Science, Southwest University, Chongqing 400715, P.R. China.
authors.forename: Huayi
authors.initials: H
authors.lastname: Suo
authors.affiliation: College of Food Science, Southwest University, Chongqing 400715, P.R. China.
authors.forename: Muying
authors.initials: M
authors.lastname: DU
authors.affiliation: Department of Biological and Chemical Engineering, Chongqing University of Education, Chongqing 400067, P.R. China.
authors.forename: Xin
authors.initials: X
authors.lastname: Zhao
authors.affiliation: Institute of Qinghai-Tibetan Plateau, Southwest University for Nationalities, Chengdu, Sichuan 610041, P.R. China.
authors.forename: Jian
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Biological and Chemical Engineering, Chongqing University of Education, Chongqing 400067, P.R. China.
authors.forename: Gui-Jie
authors.initials: GJ
authors.lastname: Li
authors.affiliation: Department of Food Science and Nutrition, Pusan National University, Busan 609-735, Republic of Korea.
authors.forename: Jia-Li
authors.initials: JL
authors.lastname: Song
authors.affiliation: Xinjiang Academy of Agricultural Sciences, Urumqi, Xinjiang 830091, P.R. China.
authors.forename: Zhenhu
authors.initials: Z
authors.lastname: Liu
keywords: Lactobacillus fermentum Lee; activated carbon; bisacodyl; constipation; gastrointestinal transit