title: Blood-brain barrier proteomics: towards the understanding of neurodegenerative diseases.
abstract: The blood-brain barrier (BBB) regulates the passage of endogenous and exogenous compounds and thus contributes to the brain homeostasis with the help of well-known proteins such as tight junction proteins, plasma membrane transporters and metabolic barrier proteins. In the last decade, proteomics have emerged as supplementary tools for BBB research. The development of proteomic technologies has provided several means to extend knowledge on the BBB and to investigate additional routes for the bypass of this barrier. Proteomics approaches have been used in vivo and also using in vitro BBB models to decipher the physiological characteristics and, under stress conditions, to understand the molecular mechanisms of brain diseases. This work has demonstrated that both quantitative global and targeted proteomics approaches are powerful and provide significant information on the brain microvessel endothelium. However, current knowledge is only partial and it is necessary to increase the studies using proteomics tools that will provide additional information concerning brain pathologies or BBB metabolism. Highly sensitive, accurate and specific protein quantification by quantitative targeted proteomics appears as an essential methodology for human BBB studies.
authors.affiliation: Laboratoire de la Barrière Hématoencéphalique, Lens, France. Electronic address: yannis.karamanos@univ-artois.fr.
authors.forename: Yannis
authors.initials: Y
authors.lastname: Karamanos
authors.affiliation: Laboratoire de la Barrière Hématoencéphalique, Lens, France.
authors.forename: Fabien
authors.initials: F
authors.lastname: Gosselet
authors.affiliation: Laboratoire de la Barrière Hématoencéphalique, Lens, France.
authors.forename: Marie-Pierre
authors.initials: MP
authors.lastname: Dehouck
authors.affiliation: Laboratoire de la Barrière Hématoencéphalique, Lens, France.
authors.forename: Roméo
authors.initials: R
authors.lastname: Cecchelli
mesh_terms: D001692:Biological Transport; D001812:Blood-Brain Barrier / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D004730:Endothelium, Vascular / Q000378:metabolism* / Q000502:physiology; D006801:Humans; D055806:Microvessels / Q000502:physiology; D019636:Neurodegenerative Diseases / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*
keywords: BBB; Blood–brain barrier; Endothelial cells; Neurodegenerative disease; Proteomics