title: Polymorphisms in RNA sensing toll like receptor genes and its association with clinical outcomes of dengue virus infection.
abstract: Functional polymorphisms in RNA recognizing toll like receptors (TLR) 3, 7, 8 and toll-interleukin-1 receptor domain containing adapter protein adapter (TIRAP) coding genes were investigated in 120 dengue cases [87 dengue fever (DF) cases and 33 dengue hemorrhagic fever (DHF) cases] and 109 healthy controls (HC) to identify their association with clinical outcomes of dengue virus infection. Results revealed significantly lower frequency of TLR3 rs3775291 T allele [DHF vs. DF P = 0.015 odds ratio (OR) with 95% confidence interval (CI) 0.390 (0.160–0.880); DHF vs. HC P = 0.018 OR with 95% CI 0.410 (0.170–0.900)] and ‘T’ allele carriers [DHF vs. DF P = 0.008 OR with 95% CI 0.288 (0.115–0.722); DHF vs. HC P = 0.040 OR with 95% CI 0.393 (0.162–0.956)] and higher frequency of TIRAP rs8177374 ‘C/T’ genotype [DHF vs. HC P = 0.020 OR with 95% CI 2.643 (1.167–5.986)] in DHF. Higher frequency of TLR8 rs3764879–rs3764880 haplotype C-A was observed in male DF cases compared to male HC [P = 0.025 OR with 95% CI 2.185 (1.101–4.336)]. The results suggest that TLR3 and TIRAP gene variants influence the risk for DHF.
authors.affiliation: Dengue Group, National Institute of Virology (ICMR), 20A, Dr. Ambedkar Road, Pune 411001, Maharashtra, India. alagarasu@gmail.com
authors.forename: K
authors.initials: K
authors.lastname: Alagarasu
authors.forename: R V
authors.initials: RV
authors.lastname: Bachal
authors.forename: R S
authors.initials: RS
authors.lastname: Memane
authors.forename: P S
authors.initials: PS
authors.lastname: Shah
authors.forename: D
authors.initials: D
authors.lastname: Cecilia
mesh_terms: D000293:Adolescent; D000328:Adult; D000483:Alleles; D016022:Case-Control Studies; D019295:Computational Biology; D003715:Dengue / Q000235:genetics* / Q000821:virology; D003716:Dengue Virus; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D008297:Male; D008562:Membrane Glycoproteins / Q000235:genetics; D008875:Middle Aged; D063868:Patient Outcome Assessment; D011110:Polymorphism, Genetic; D020641:Polymorphism, Single Nucleotide; D011379:Prognosis; D017472:Receptors, Interleukin-1 / Q000235:genetics; D051193:Toll-Like Receptors / Q000235:genetics*; D064878:Web Browser; D055815:Young Adult
chemical_list: D008562:Membrane Glycoproteins; D017472:Receptors, Interleukin-1; C434984:TIRAP protein, human; D051193:Toll-Like Receptors