title: Proteomic profile in Perna viridis after exposed to Prorocentrum lima, a  dinoflagellate producing DSP toxins.
abstract: In the current study, we compared protein profiles in gills of Perna viridis after exposure to Prorocentrumlima, a dinoflagellate producing DSP toxins, and identified the differential abundances of protein spots using 2D-electrophoresis. After exposure to P. lima, the level of okadaic acid (a main component of DSP toxins) in gills of P. viridis significantly increased at 6 h, but mussels were all apparently healthy without death. Among the 28 identified protein spots by MALDI TOF/TOF-MS, 12 proteins were up-regulated and 16 were down-regulated in the P. lima-exposed mussels. These identified proteins were involved in various biological activities, such as metabolism, cytoskeleton, signal transduction, response to oxidative stress and detoxification. Taken together, our results indicated that the presence of P. lima caused DSP toxins accumulation in mussel gill, and might consequently induce cytoskeletonal disorganization,oxidative stress, a dysfunction in metabolism and ubiquitination/proteasome activity.
authors.affiliation: College of Life Science and Technology, Jinan University, Guangzhou 510632, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Huang
authors.forename: Ying
authors.initials: Y
authors.lastname: Zou
authors.forename: Hui-wen
authors.initials: HW
authors.lastname: Weng
authors.forename: Hong-Ye
authors.initials: HY
authors.lastname: Li
authors.forename: Jie-Sheng
authors.initials: JS
authors.lastname: Liu
authors.forename: Wei-Dong
authors.initials: WD
authors.lastname: Yang
mesh_terms: D000818:Animals; D049872:Bivalvia / Q000378:metabolism; D004141:Dinoflagellida / Q000502:physiology*; D005880:Gills / Q000378:metabolism; D008387:Marine Toxins / Q000633:toxicity*; D019319:Okadaic Acid; D049883:Perna / Q000378:metabolism / Q000502:physiology*; D020543:Proteome / Q000378:metabolism*; D040901:Proteomics; D014873:Water Pollutants / Q000633:toxicity*
chemical_list: D008387:Marine Toxins; D020543:Proteome; D014873:Water Pollutants; D019319:Okadaic Acid