title: Isolation and characterization of a novel lectin from the edible mushroom Stropharia rugosoannulata.
abstract: To date, only a few steroids have been isolated from the mushroom Stropharia rugosoannulata which can be cultivated. In this paper, a novel lectin (SRL) with a molecular weight of 38 kDa, and a unique IKSGVYRIVSWQGALGPEAR N-terminal sequence was isolated from S. rugosoannulata, which represents the first protein isolated from the mushroom. The purification methods included (NH4)2SO4 precipitation, ion exchange chromatography on CM-cellulose, Q-Sepharose, and SP-Sepharose, and gel- filtration on Superdex-75. The lectin was adsorbed on all three types of ion exchangers and was purified more than 450-fold. The lectin was stable below 70 °C (with half of the activity preserved at 80 °C), and in the presence of NaOH and HCl solutions up to a concentration of 12.5 mM and 25 mM, respectively. The hemagglutinating activity of SRL was inhibited by inulin. Cd2+ and Hg2+ ions strongly reduced the hemagglutinating activity at concentrations from 1.25 mM to 10 mM. SRL exhibited anti-proliferative activity toward both hepatoma Hep G2 cells and leukemia L1210 cells, with an IC50 of 7 μM and 19 μM, respectively. The activity of HIV-1 reverse transcriptase could also be inhibited by SRL, with an IC50 of 10 μM.
authors.affiliation: State Key Laboratory for Agrobiotechnology and Department of Microbiology, China Agricultural University, Beijing 100193, China. zhangweiweicau@gmail.com.
authors.forename: Weiwei
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Institute of Biotechnology and Germplasmic Resource, Yunnan Academy of Agricultural Science, Kunming 650223, China. tiangt@aliyun.com.
authors.forename: Guoting
authors.initials: G
authors.lastname: Tian
authors.affiliation: State Key Laboratory for Agrobiotechnology and Department of Microbiology, China Agricultural University, Beijing 100193, China. gengxueran2007@163.com.
authors.forename: Xueran
authors.initials: X
authors.lastname: Geng
authors.affiliation: Institute of Biotechnology and Germplasmic Resource, Yunnan Academy of Agricultural Science, Kunming 650223, China. yaasmushroom@aliyun.com.
authors.forename: Yongchang
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China. b021770@mailserv.cuhk.edu.hk.
authors.forename: Tzi Bun
authors.initials: TB
authors.lastname: Ng
authors.affiliation: College of Food Science and Technology, Nanjing Agricultural University, Weigang, Nanjing 210095, China. zhlychen@njau.edu.cn.
authors.forename: Liyan
authors.initials: L
authors.lastname: Zhao
authors.affiliation: State Key Laboratory for Agrobiotechnology and Department of Microbiology, China Agricultural University, Beijing 100193, China. hxwang1957@gmail.com.
authors.forename: Hexiang
authors.initials: H
authors.lastname: Wang
mesh_terms: D000363:Agaricales / Q000737:chemistry*; D000595:Amino Acid Sequence; D000818:Animals; D002241:Carbohydrates / Q000494:pharmacology; D002412:Cations / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D054303:HIV Reverse Transcriptase / Q000037:antagonists & inhibitors / Q000378:metabolism; D006384:Hemagglutination / Q000187:drug effects; D056945:Hep G2 Cells; D006801:Humans; D006851:Hydrochloric Acid / Q000494:pharmacology; D020128:Inhibitory Concentration 50; D037102:Lectins / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D051379:Mice; D008969:Molecular Sequence Data; D011817:Rabbits; D012972:Sodium Hydroxide / Q000494:pharmacology; D013696:Temperature
chemical_list: D002241:Carbohydrates; D002412:Cations; D037102:Lectins; D012972:Sodium Hydroxide; C514824:reverse transcriptase, Human immunodeficiency virus 1; D054303:HIV Reverse Transcriptase; D006851:Hydrochloric Acid