title: The determination of possible genetic damage to women undergoing in vitro fertilization due to infertility caused by the male factor.
abstract: In this study, we aimed at determining possible genetic damage to women who were exposed to in vitro fertilization (IVF) due to infertility with male factor. Four different genotoxicity tests were used in human lymphocytes in this study with regard to chromosomal aberration (CA), sister chromatid exchange (SCE), micronucleus (MN), and comet tests. There was a statistically significant increase in sister chromatid exchange (SCE) test in the study group compared with the control group. In addition, a higher rate of MN frequency was determined only in the 21–30 age range study group compared with the control group in the same age range. On the other hand, MN frequency did not differ significantly between the control and total study groups. In addition, there was no significant difference between the control group and the study group in terms of mitotic (MI), replication (RI), and nuclear division (NDI) indices. Furthermore, there was no statistically significant increase for chromosomal aberration and DNA damage to the study groups. Our results showed that in vitro fertilization treatments have a weak risk at the genetic level in cultured human lymphocytes.
authors.affiliation: Zekai Tahir Burak Women’s Health Education and Resarch Hospital, Ankara, Turkey.
authors.forename: S
authors.initials: S
authors.lastname: Özden
authors.forename: D
authors.initials: D
authors.lastname: Yüzbaşıoğlu
authors.forename: F
authors.initials: F
authors.lastname: Ünal
authors.forename: M
authors.initials: M
authors.lastname: Özel
mesh_terms: D000328:Adult; D000375:Aging / Q000502:physiology; D002869:Chromosome Aberrations / Q000187:drug effects; D020552:Comet Assay; D004249:DNA Damage; D005260:Female; D005307:Fertilization in Vitro / Q000009:adverse effects*; D006801:Humans; D007247:Infertility, Female / Q000235:genetics*; D007248:Infertility, Male / Q000235:genetics*; D008214:Lymphocytes / Q000187:drug effects; D008297:Male; D015162:Micronucleus Tests; D008940:Mitotic Index; D009152:Mutagenicity Tests; D009154:Mutation / Q000187:drug effects*; D012854:Sister Chromatid Exchange / Q000187:drug effects; D055815:Young Adult