title: Reduction of photo bleaching and long term archiving of chemically cleared GFP-expressing mouse brains.
abstract: Tissue clearing allows microscopy of large specimens as whole mouse brains or embryos. However, lipophilic tissue clearing agents as dibenzyl ether limit storage time of GFP-expressing samples to several days and do not prevent them from photobleaching during microscopy. To preserve GFP fluorescence, we developed a transparent solid resin formulation, which maintains the specimens' transparency and provides a constant signal to noise ratio even after hours of continuous laser irradiation. If required, high-power illumination or long exposure times can be applied with virtually no loss in signal quality and samples can be archived for years.
authors.affiliation: Vienna University of Technology, FKE, Chair of Bioelectronics, Vienna, Austria; Center for Brain Research, Medical University of Vienna, Vienna, Austria.
authors.forename: Klaus
authors.initials: K
authors.lastname: Becker
authors.affiliation: Vienna University of Technology, FKE, Chair of Bioelectronics, Vienna, Austria; Center for Brain Research, Medical University of Vienna, Vienna, Austria.
authors.forename: Christian Markus
authors.initials: CM
authors.lastname: Hahn
authors.affiliation: Vienna University of Technology, FKE, Chair of Bioelectronics, Vienna, Austria; Center for Brain Research, Medical University of Vienna, Vienna, Austria.
authors.forename: Saiedeh
authors.initials: S
authors.lastname: Saghafi
authors.affiliation: Vienna University of Technology, FKE, Chair of Bioelectronics, Vienna, Austria; Center for Brain Research, Medical University of Vienna, Vienna, Austria.
authors.forename: Nina
authors.initials: N
authors.lastname: Jährling
authors.affiliation: Vienna University of Technology, FKE, Chair of Bioelectronics, Vienna, Austria; Center for Brain Research, Medical University of Vienna, Vienna, Austria.
authors.forename: Martina
authors.initials: M
authors.lastname: Wanis
authors.affiliation: Vienna University of Technology, FKE, Chair of Bioelectronics, Vienna, Austria; Center for Brain Research, Medical University of Vienna, Vienna, Austria.
authors.forename: Hans-Ulrich
authors.initials: HU
authors.lastname: Dodt
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism* / Q000473:pathology / Q000528:radiation effects; D049452:Green Fluorescent Proteins / Q000032:analysis / Q000737:chemistry*; D007834:Lasers; D051379:Mice; D008853:Microscopy / Q000379:methods; D038761:Photobleaching; D012117:Resins, Synthetic; D016610:Tissue Embedding / Q000379:methods*; D014021:Tissue Preservation
chemical_list: D012117:Resins, Synthetic; D049452:Green Fluorescent Proteins