title: Evolving P450pyr monooxygenase for highly regioselective terminal hydroxylation of n-butanol to 1,4-butanediol.
abstract: Directed evolution of P450pyr created I83M/I82T mutant for highly regioselective terminal hydroxylation of n-butanol to 1,4-butanediol, representing the first achievement of this hydroxylation reaction by chemical or enzymatic methods and an unique example of evolving a hydroxylase to switch the substrate acceptance from a hydrophobic to hydrophilic compound.
authors.affiliation: Department of Chemical, Biomolecular Engineering, National University of Singapore, 4 Engineering Drive 4, Singapore117585. chelz@nus.edu.sg.
authors.forename: Yi
authors.initials: Y
authors.lastname: Yang
authors.forename: Yu Tse
authors.initials: YT
authors.lastname: Chi
authors.forename: Hui Hung
authors.initials: HH
authors.lastname: Toh
authors.forename: Zhi
authors.initials: Z
authors.lastname: Li
mesh_terms: D020001:1-Butanol / Q000737:chemistry* / Q000378:metabolism*; D002072:Butylene Glycols / Q000378:metabolism*; D019020:Directed Molecular Evolution; D057927:Hydrophobic and Hydrophilic Interactions; D006900:Hydroxylation; D006899:Mixed Function Oxygenases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D062105:Molecular Docking Simulation; D011487:Protein Conformation; D020578:Sphingomonas / Q000201:enzymology; D013237:Stereoisomerism; D013379:Substrate Specificity
chemical_list: D002072:Butylene Glycols; C039681:1,4-butanediol; D020001:1-Butanol; D006899:Mixed Function Oxygenases