title: Targeted analytical toxicology: simultaneous determination of 17α-ethynylestradiol and the estrogen-induced vitellogenin biomarker.
abstract: In most toxicological studies, chemical concentrations and its biological/molecular response (biomarker) levels are often quantified separately. We report a method, named as Targeted Analytical Toxicology (TAT), for the simultaneous determination of levels of chemicals and protein biomarkers in organisms by UPLC/ESI-MS/MS on the basis of targeted proteomics. The TAT method is successfully applied to detect the concentrations of 17α-ethynylestradiol (EE2) and EE2-induced vitellogenin (a biomarker for estrogens) in male zebrafish exposed to EE2. It is expected for TAT to have broad application in toxicology, especially in environmental toxicology, due to its high sensitivity, accuracy, reproducibility, high throughput, and the savings of time, cost and labor.
authors.affiliation: Institute of Environmental Sciences, College of Environmental and Resource Sciences, Zhejiang University, Hangzhou 310058, China; Department of Effect-Directed Analysis, Helmholtz Centre for Environmental Research - UFZ, Leipzig 04318, Germany.
authors.forename: Fangxing
authors.initials: F
authors.lastname: Yang
authors.affiliation: Institute of Environmental Sciences, College of Environmental and Resource Sciences, Zhejiang University, Hangzhou 310058, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Huang
authors.affiliation: Institute of Environmental Sciences, College of Environmental and Resource Sciences, Zhejiang University, Hangzhou 310058, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Xie
authors.affiliation: Department of Environmental Health, Harvard School of Public Health, Boston, MA 02215, USA.
authors.forename: Chensheng
authors.initials: C
authors.lastname: Lu
authors.affiliation: Institute of Environmental Sciences, College of Environmental and Resource Sciences, Zhejiang University, Hangzhou 310058, China; MOE Key Laboratory of Environmental Remediation and Ecosystem Health, College of Environmental and Resource Sciences, Zhejiang University, Hangzhou 310058, China. Electronic address: wliu@zju.edu.cn.
authors.forename: Weiping
authors.initials: W
authors.lastname: Liu
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000032:analysis / Q000378:metabolism; D002853:Chromatography, Liquid / Q000379:methods; D054750:Ecotoxicology / Q000379:methods; D004785:Environmental Pollutants / Q000032:analysis* / Q000378:metabolism; D004967:Estrogens / Q000032:analysis*; D004997:Ethinyl Estradiol / Q000032:analysis*; D008297:Male; D040901:Proteomics / Q000379:methods*; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D053719:Tandem Mass Spectrometry / Q000379:methods*; D014819:Vitellogenins / Q000032:analysis* / Q000096:biosynthesis; D015027:Zebrafish / Q000378:metabolism
chemical_list: D015415:Biomarkers; D004785:Environmental Pollutants; D004967:Estrogens; D014819:Vitellogenins; D004997:Ethinyl Estradiol
keywords: 17α-Ethynylestradiol; Environmental toxicology; Protein biomarker; Simultaneous determination; Targeted analytical toxicology; Vitellogenin