title: Exploring time of death from potassium, sodium, chloride, glucose & calcium analysis of postmortem synovial fluid in semi arid climate.
abstract: Estimation of time of death (TOD) with fair accuracy from postmortem changes still remains an important but difficult task to be performed by every autopsy surgeon under different climatic conditions. The environment plays an important role in the process of decomposition and thereby affecting the levels of electrolytes and other biochemical parameters in the postmortem samples. Since, there is limited information available on the levels of these biochemical parameters from semi arid environment, the present study was aimed to explore time of death by analyzing electrolyte, glucose and calcium levels of postmortem synovial fluid collected from samples under such climatic conditions. The synovial fluid samples from two hundred and ten bodies brought to University College of Medical Sciences and associated Guru Teg Bahadur Hospital Delhi for medico-legal postmortem examination, during the period of November 2010 to April 2012, were analyzed for potassium, sodium, chloride, glucose and calcium. Univariate regression analysis of electrolyte concentrations of synovial fluid showed significant positive relationship between time of death and potassium (r = 0.840, p = 0.000). However, there was negative relationship between time of death and sodium (r = -0.175, p = 0.011) & glucose (r = -0.427, p = 0.000) and no significant relationship was found between time of death and calcium (r = 0.099, p = 0.152) & chloride (r = 0.082, p = 0.24) among the samples analyzed.
authors.affiliation: Dept. of Forensic Medicine, University College of Medical Sciences, Delhi, India.
authors.forename: Arun K
authors.initials: AK
authors.lastname: Siddhamsetty
authors.affiliation: Dept. of Forensic Medicine, University College of Medical Sciences, Delhi, India. Electronic address: vermasatish2003@gmail.com.
authors.forename: Satish K
authors.initials: SK
authors.lastname: Verma
authors.affiliation: Dept. of Forensic Medicine, University College of Medical Sciences, Delhi, India.
authors.forename: Anil
authors.initials: A
authors.lastname: Kohli
authors.affiliation: Dept. of Public Health Dentistry, Kalka Dental College, Meerut, U.P., India.
authors.forename: Aditi
authors.initials: A
authors.lastname: Verma
authors.affiliation: Dept. of Biochemistry, University College of Medical Sciences, Delhi, India.
authors.forename: Dinesh
authors.initials: D
authors.lastname: Puri
authors.affiliation: Dept. of Biochemistry, University College of Medical Sciences, Delhi, India.
authors.forename: Archana
authors.initials: A
authors.lastname: Singh
mesh_terms: D001344:Autopsy; D002118:Calcium / Q000032:analysis; D002712:Chlorides / Q000032:analysis; D003889:Desert Climate; D004573:Electrolytes / Q000032:analysis*; D005260:Female; D005554:Forensic Medicine; D005947:Glucose / Q000032:analysis; D006801:Humans; D007194:India; D008297:Male; D011180:Postmortem Changes; D011188:Potassium / Q000032:analysis; D012964:Sodium / Q000032:analysis; D013582:Synovial Fluid / Q000737:chemistry*; D013997:Time Factors
chemical_list: D002712:Chlorides; D004573:Electrolytes; D012964:Sodium; D005947:Glucose; D011188:Potassium; D002118:Calcium
keywords: Postmortem biochemistry; Postmortem interval; Semi arid climate; Synovial fluid; Time of death