title: Characterisation of translation elongation factor eEF1B subunit expression in mammalian cells and tissues and co-localisation with eEF1A2.
abstract: Translation elongation is the stage of protein synthesis in which the translation factor eEF1A plays a pivotal role that is dependent on GTP exchange. In vertebrates, eEF1A can exist as two separately encoded tissue-specific isoforms, eEF1A1, which is almost ubiquitously expressed, and eEF1A2, which is confined to neurons and muscle. The GTP exchange factor for eEF1A1 is a complex called eEF1B made up of subunits eEF1Bα, eEF1Bδ and eEF1Bγ. Previous studies have cast doubt on the ability of eEF1B to interact with eEF1A2, suggesting that this isoform might use a different GTP exchange factor. We show that eEF1B subunits are all widely expressed to varying degrees in different cell lines and tissues, and at different stages of development. We show that ablation of any of the subunits in human cell lines has a small but significant impact on cell viability and cycling. Finally, we show that both eEF1A1 and eEF1A2 colocalise with all eEF1B subunits, in such close proximity that they are highly likely to be in a complex.
authors.affiliation: Centre for Genomic and Experimental Medicine, University of Edinburgh, Institute of Genetics and Molecular Medicine, Western General Hospital, Edinburgh, United Kingdom.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Cao
authors.affiliation: Centre for Genomic and Experimental Medicine, University of Edinburgh, Institute of Genetics and Molecular Medicine, Western General Hospital, Edinburgh, United Kingdom.
authors.forename: Miriam
authors.initials: M
authors.lastname: Portela
authors.affiliation: Centre for Genomic and Experimental Medicine, University of Edinburgh, Institute of Genetics and Molecular Medicine, Western General Hospital, Edinburgh, United Kingdom.
authors.forename: Justyna
authors.initials: J
authors.lastname: Janikiewicz
authors.affiliation: Centre for Genomic and Experimental Medicine, University of Edinburgh, Institute of Genetics and Molecular Medicine, Western General Hospital, Edinburgh, United Kingdom.
authors.forename: Jennifer
authors.initials: J
authors.lastname: Doig
authors.affiliation: Centre for Genomic and Experimental Medicine, University of Edinburgh, Institute of Genetics and Molecular Medicine, Western General Hospital, Edinburgh, United Kingdom.
authors.forename: Catherine M
authors.initials: CM
authors.lastname: Abbott
mesh_terms: D000818:Animals; D002460:Cell Line; D002470:Cell Survival; D020662:Guanine Nucleotide Exchange Factors / Q000032:analysis* / Q000235:genetics; D006801:Humans; D051379:Mice; D020648:Peptide Elongation Factor 1 / Q000032:analysis* / Q000235:genetics; D034622:RNA Interference
chemical_list: C503234:EEF1A2 protein, human; D020662:Guanine Nucleotide Exchange Factors; D020648:Peptide Elongation Factor 1; C000599473:eEF1B-alpha protein, human; C000599474:eEF1B-beta protein, human; C000599475:eEF1B-gamma protein, human