title: Highly sensitive SERS-based immunoassay of aflatoxin B1 using silica-encapsulated hollow gold nanoparticles.
abstract: Aflatoxin B1 (AFB1) is a well-known carcinogenic contaminant in foods. It is classified as an extremely hazardous compound because of its potential toxicity to the human nervous system. AFB1 has also been extensively used as a biochemical marker to evaluate the degree of food spoilage. In this study, a novel surface-enhanced Raman scattering (SERS)-based immunoassay platform using silica-encapsulated hollow gold nanoparticles (SEHGNs) and magnetic beads was developed for highly sensitive detection of AFB1. SEHGNs were used as highly stable SERS-encoding nano tags, and magnetic beads were used as supporting substrates for the high-density loading of immunocomplexes. Quantitative analysis of AFB1 was performed by monitoring the intensity change of the characteristic peaks of Raman reporter molecules. The limit of detection (LOD) of AFB1, determined by this SERS-based immunoassay, was determined to be 0.1 ng/mL. This method has some advantages over other analytical methods with respect to rapid analysis (less than 30 min), good selectivity, and reproducibility. The proposed method is expected to be a new analytical tool for the trace analysis of various mycotoxins.
authors.affiliation: Department of Bionano Technology, Hanyang University, Ansan 426791, South Korea.
authors.forename: Juhui
authors.initials: J
authors.lastname: Ko
authors.affiliation: Department of Electronics and Communication Engineering, Hanyang University, Ansan 426791, South Korea. Electronic address: cklee@hanyang.ac.kr.
authors.forename: Chankil
authors.initials: C
authors.lastname: Lee
authors.affiliation: Department of Bionano Technology, Hanyang University, Ansan 426791, South Korea. Electronic address: jbchoo@hanyang.ac.kr.
authors.forename: Jaebum
authors.initials: J
authors.lastname: Choo
mesh_terms: D016604:Aflatoxin B1 / Q000032:analysis* / Q000276:immunology; D000906:Antibodies / Q000737:chemistry / Q000276:immunology; D006046:Gold / Q000737:chemistry*; D007118:Immunoassay; D053768:Metal Nanoparticles / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry*
chemical_list: D000906:Antibodies; D006046:Gold; D012822:Silicon Dioxide; D016604:Aflatoxin B1
keywords: Aflatoxin B1; Magnetic bead; Mycotoxin; Silica-encapsulation; Surface-enhanced Raman scattering