title: Rhelogical, dermal wound healing and in vitro antioxidant properties of exopolysaccharide hydrogel from Pseudomonas stutzeri AS22.
abstract: The in vitro antioxidant activity and the in vivo wound healing performance of the exopolysaccharide EPS22, produced by Pseudomonas stutzeri AS22, were investigated. Antioxidant activity was evaluated by three different tests. The scavenging effect on DPPH radicals at a concentration of 1mg/ml was 80±1.41%. The reducing power reached a maximum of 1.26±0.02 at 2 mg/ml. Moreover, EPS22 showed good chelating ability and chelated almost 88.5±0.7% of ferrous ions at 0.75 mg/ml. The rheological characterization of EPS22 gel (0.5%) showed a pseudoplastic behavior, high elasticity, good mechanical strength and stability with high water-absorption ability. The application of the EPS22 gel on dermal full-thickness excision wounds in a rat model every two days, enhanced significantly wound healing activity and a total closure was achieved after 12 days of wound induction. Further, histological examination of biopsies showed advanced tissue regeneration, characterized by the presence of well-organized stratum of both derma and epidermis.
authors.affiliation: Enzyme Engineering and Microbiology Laboratory, Sfax - University, National School of Engineering of Sfax (ENIS), BP 1173, Sfax 3038, Tunisia.
authors.forename: Hana
authors.initials: H
authors.lastname: Maalej
authors.affiliation: Laboratory of Pharmacology, Faculty of Medicine of Sfax, Road Majida Boulila, 3028 Sfax, Tunisia.
authors.forename: Dorsaf
authors.initials: D
authors.lastname: Moalla
authors.affiliation: Centre de Recherches sur les Macromolécules Végétales, C.N.R.S., Université Joseph Fourier, BP 53, Grenoble Cedex 9 38041, France.
authors.forename: Claire
authors.initials: C
authors.lastname: Boisset
authors.affiliation: Laboratory of Pharmacology, Faculty of Medicine of Sfax, Road Majida Boulila, 3028 Sfax, Tunisia.
authors.forename: Sana
authors.initials: S
authors.lastname: Bardaa
authors.affiliation: Enzyme Engineering and Microbiology Laboratory, Sfax - University, National School of Engineering of Sfax (ENIS), BP 1173, Sfax 3038, Tunisia.
authors.forename: Hanen
authors.initials: H
authors.lastname: Ben Ayed
authors.affiliation: Laboratory of Pharmacology, Faculty of Medicine of Sfax, Road Majida Boulila, 3028 Sfax, Tunisia.
authors.forename: Zouheir
authors.initials: Z
authors.lastname: Sahnoun
authors.affiliation: Laboratory of Histology Embryology, Faculty of Medicine of Sfax, Road Majida Boulila, 3028 Sfax, Tunisia.
authors.forename: Tarek
authors.initials: T
authors.lastname: Rebai
authors.affiliation: Enzyme Engineering and Microbiology Laboratory, Sfax - University, National School of Engineering of Sfax (ENIS), BP 1173, Sfax 3038, Tunisia.
authors.forename: Moncef
authors.initials: M
authors.lastname: Nasri
authors.affiliation: Enzyme Engineering and Microbiology Laboratory, Sfax - University, National School of Engineering of Sfax (ENIS), BP 1173, Sfax 3038, Tunisia. Electronic address: hmidet_noomen@yahoo.fr.
authors.forename: Noomen
authors.initials: N
authors.lastname: Hmidet
mesh_terms: D000279:Administration, Cutaneous; D000818:Animals; D000975:Antioxidants / Q000737:chemistry* / Q000494:pharmacology*; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry* / Q000494:pharmacology*; D011135:Polysaccharides, Bacterial / Q000737:chemistry* / Q000494:pharmacology*; D044223:Pseudomonas stutzeri / Q000737:chemistry*; D051381:Rats; D012867:Skin / Q000187:drug effects / Q000378:metabolism; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D000975:Antioxidants; D011135:Polysaccharides, Bacterial; C064841:exopolysaccharide, Pseudomonas; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate
keywords: Antioxidant activity; Exopolysccharide EPS22; Pseudomonas stutzeri AS22; Rheological characterization; Wound healing