title: Electrospun nitric oxide releasing bandage with enhanced wound healing.
abstract: Research has shown that nitric oxide (NO) enhances wound healing. The incorporation of NO into polymers for medical materials and surgical devices has potential benefits for many wound healing applications. In this work, acrylonitrile (AN)-based terpolymers were electrospun to form non-woven sheets of bandage or wound dressing type materials. NO is bound to the polymer backbone via the formation of a diazeniumdiolate group. In a 14 day NO release study, the dressings released 79 μmol NO g(-1) polymer. The NO-loaded dressings were tested for NO release in vivo, which demonstrate upregulation of NO-inducible genes with dressing application compared to empty dressings. Studies were also conducted to evaluate healing progression in wounds with dressing application performed weekly and daily. In two separate studies, excisional wounds were created on the dorsa of 10 mice. Dressings with NO loaded on the fibers or empty controls were applied to the wounds and measurements of the wound area were taken at each dressing change. The data show significantly enhanced healing progression in the wounds with weekly NO application, which is more dramatic with daily application. Further, the application of daily NO bandages results in improved wound vascularity. These data demonstrate the potential for this novel NO-releasing dressing as a valid wound healing therapy.
authors.affiliation: Department of Chemistry, University of Texas at Dallas, 800 West Campbell Road, Mailstop BE26, Richardson, TX 75080-3021, USA.
authors.forename: A
authors.initials: A
authors.lastname: Lowe
authors.affiliation: Department of Plastic Surgery, University of Texas at Southwestern, 5323 Harry Hines Blvd, Dallas, TX 75390, USA.
authors.forename: J
authors.initials: J
authors.lastname: Bills
authors.affiliation: Department of Chemistry, University of Texas at Dallas, 800 West Campbell Road, Mailstop BE26, Richardson, TX 75080-3021, USA.
authors.forename: R
authors.initials: R
authors.lastname: Verma
authors.affiliation: Department of Plastic Surgery, University of Texas at Southwestern, 5323 Harry Hines Blvd, Dallas, TX 75390, USA.
authors.forename: L
authors.initials: L
authors.lastname: Lavery
authors.affiliation: Department of Plastic Surgery, University of Texas at Southwestern, 5323 Harry Hines Blvd, Dallas, TX 75390, USA. Electronic address: kathryn.davis@utsouthwestern.edu.
authors.forename: K
authors.initials: K
authors.lastname: Davis
authors.affiliation: Department of Chemistry, University of Texas at Dallas, 800 West Campbell Road, Mailstop BE26, Richardson, TX 75080-3021, USA. Electronic address: balkus@utdallas.edu.
authors.forename: K J
authors.initials: KJ
authors.lastname: Balkus
mesh_terms: D000818:Animals; D001458:Bandages; D049109:Cell Proliferation / Q000187:drug effects; D003094:Collagen / Q000096:biosynthesis; D003692:Delayed-Action Preparations / Q000737:chemistry / Q000494:pharmacology; D042783:Endothelial Cells / Q000378:metabolism / Q000473:pathology; D019344:Lactic Acid / Q000737:chemistry / Q000494:pharmacology; D008297:Male; D051379:Mice; D032389:Myocytes, Smooth Muscle / Q000378:metabolism / Q000473:pathology; D018377:Neurotransmitter Agents / Q000737:chemistry / Q000494:pharmacology; D009569:Nitric Oxide / Q000737:chemistry / Q000494:pharmacology; D011091:Polyesters; D011108:Polymers / Q000737:chemistry / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D023822:Tissue Engineering; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D003692:Delayed-Action Preparations; D018377:Neurotransmitter Agents; D011091:Polyesters; D011108:Polymers; D009569:Nitric Oxide; D019344:Lactic Acid; C033616:poly(lactide); D003094:Collagen
keywords: Diazeniumdiolate; Electrospinning; Nitric oxide; Polyacrylonitrile; Wound healing