title: Applications of mass spectrometry to the study of protein aggregation.
abstract: Mass spectrometry is an analytical technique that measures the mass-to-charge ratio of charged particles. Nowadays mass spectrometry-based approaches play a pivotal role in both detection and characterization of proteins. Here we describe two applications to study insoluble proteins: (a) hydrogen/deuterium exchange combined with mass spectrometry to analyze structural properties of amyloid fibrils and (b) the screening for inhibitors of the aggregation process by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.
authors.affiliation: Servei de Proteòmica i Biologia Estructural, Universitat Autònoma de Barcelona, Bellaterra (Cerdanyola del Vallès), 08193, Barcelona, Spain, Silvia.Bronsoms@uab.cat.
authors.forename: Sílvia
authors.initials: S
authors.lastname: Bronsoms
authors.forename: Sebastián A
authors.initials: SA
authors.lastname: Trejo
mesh_terms: D000682:Amyloid / Q000737:chemistry*; D000818:Animals; D003903:Deuterium / Q000737:chemistry; D041961:Deuterium Exchange Measurement / Q000379:methods*; D006801:Humans; D006859:Hydrogen / Q000737:chemistry; D066329:Protein Aggregates / Q000502:physiology*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
chemical_list: D000682:Amyloid; D066329:Protein Aggregates; D006859:Hydrogen; D003903:Deuterium