title: Aptasensor for electrochemical sensing of angiogenin based on electrode modified by cationic polyelectrolyte-functionalized graphene/gold nanoparticles composites.
abstract: Herein, a label-free and highly sensitive electrochemical aptasensor for the detection of angiogenin was proposed based on a conformational change of aptamer and amplification by poly(diallyldimethyl ammonium chloride) (PDDA)-functionalized graphene/gold nanoparticles (AuNPs) composites-modified electrode. PDDA-functionalized graphene (P-GR) nanosheets as the building block in the self-assembly of GR nanosheets/AuNPs heterostructure enhanced the electrochemical detection performance. The electrochemical aptasensor has an extraordinarily sensitive response to angiogenin in a linear range from 0.1pM to 5nM with a detection limit of 0.064pM. The developed sensor provides a promising strategy for the cancer diagnosis in medical application in the future.
authors.affiliation: Department of Chemistry, Capital Normal University, Beijing 100048, China. Electronic address: czb979216@sina.com.
authors.forename: Zhengbo
authors.initials: Z
authors.lastname: Chen
authors.affiliation: Department of Chemistry, Capital Normal University, Beijing 100048, China.
authors.forename: Chenmeng
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Department of Chemistry, Capital Normal University, Beijing 100048, China.
authors.forename: Xiaoxiao
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Chemistry, Capital Normal University, Beijing 100048, China.
authors.forename: He
authors.initials: H
authors.lastname: Ma
authors.affiliation: Department of Chemistry, Capital Normal University, Beijing 100048, China. Electronic address: wancq@cnu.edu.cn.
authors.forename: Chongqing
authors.initials: C
authors.lastname: Wan
authors.affiliation: Department of Chemistry, Capital Normal University, Beijing 100048, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Li
authors.affiliation: Department of Chemistry, Capital Normal University, Beijing 100048, China.
authors.forename: Yuqing
authors.initials: Y
authors.lastname: Lin
mesh_terms: D000498:Allyl Compounds / Q000737:chemistry; D052157:Aptamers, Nucleotide / Q000737:chemistry*; D015374:Biosensing Techniques / Q000191:economics / Q000295:instrumentation / Q000379:methods*; D055664:Electrochemical Techniques / Q000191:economics / Q000295:instrumentation / Q000379:methods; D004867:Equipment Design; D006046:Gold / Q000737:chemistry*; D006108:Graphite / Q000737:chemistry*; D006801:Humans; D057230:Limit of Detection; D053768:Metal Nanoparticles / Q000737:chemistry / Q000648:ultrastructure; D053761:Nanocomposites / Q000737:chemistry* / Q000648:ultrastructure; D009369:Neoplasms / Q000175:diagnosis; D011073:Polyamines / Q000737:chemistry*; D000071228:Polyelectrolytes; D000644:Quaternary Ammonium Compounds / Q000737:chemistry; D012259:Ribonuclease, Pancreatic / Q000032:analysis*
chemical_list: D000498:Allyl Compounds; D052157:Aptamers, Nucleotide; D006046:Gold; D006108:Graphite; D011073:Polyamines; D000071228:Polyelectrolytes; D000644:Quaternary Ammonium Compounds; D012259:Ribonuclease, Pancreatic; C508190:diallyldimethylammonium chloride; C046366:angiogenin
keywords: Angiogenin; Aptamer; Electrochemical; Gold nanoparticles; Graphene; Poly(diallyldimethyl ammonium chloride)