title: In vitro antitumor activity evaluation of some 1,2,4-triazine derivatives bearing piperazine amide moiety against breast cancer cells.
abstract: A series of 1,2,4-triazine derivatives bearing piperazine amide moiety has been synthesized and investigated for their potential anticancer activities. 1-[4-(5,6-Bis(4-subtituted phenyl)-1,2,4-triazin-3-yl)piperazin-1-yl]-2-[4-(3-substituted phenyl)piperazin-1-yl]ethanone derivative (1-32) compounds were synthesized by a four step synthetic procedure. The activity studies were evaluated using XTT method, BrdU method and flow cytometric analysis on MCF-7 breast cancer cells and NIH/3T3 (mouse embryonic fibroblast cells) healthy cells. Compounds 5 with 3-chlorophenyl and compound 7 with 4-chlorophenyl substitutions were found to be promising antiproliferative agents comparing with an effective anticancer drug, cisplatin.
authors.affiliation: Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Anadolu University, 26470 Eskisehir, Turkey. Electronic address: lyurttas@anadolu.edu.tr.
authors.forename: Leyla
authors.initials: L
authors.lastname: Yurttaş
authors.affiliation: Department of Pharmaceutical Chemistry, School of Pharmacy, Medipol University, 34083 Istanbul, Turkey.
authors.forename: Seref
authors.initials: S
authors.lastname: Demirayak
authors.affiliation: Department of Pharmaceutical Toxicology, Faculty of Pharmacy, Anadolu University, 26470 Eskisehir, Turkey.
authors.forename: Sinem
authors.initials: S
authors.lastname: Ilgın
authors.affiliation: Department of Pharmaceutical Toxicology, Faculty of Pharmacy, Anadolu University, 26470 Eskisehir, Turkey.
authors.forename: Özlem
authors.initials: Ö
authors.lastname: Atlı
mesh_terms: D000577:Amides / Q000737:chemistry*; D000818:Animals; D000970:Antineoplastic Agents / Q000138:chemical synthesis / Q000737:chemistry* / Q000633:toxicity; D017209:Apoptosis / Q000187:drug effects; D001943:Breast Neoplasms; D002945:Cisplatin / Q000633:toxicity; D005260:Female; D006801:Humans; D061986:MCF-7 Cells; D051379:Mice; D041681:NIH 3T3 Cells; D000077489:Piperazine; D010879:Piperazines / Q000737:chemistry*; D014227:Triazines / Q000138:chemical synthesis / Q000737:chemistry* / Q000633:toxicity
chemical_list: D000577:Amides; D000970:Antineoplastic Agents; D010879:Piperazines; D014227:Triazines; D000077489:Piperazine; C093444:1,2,4-triazine; D002945:Cisplatin
keywords: 1,2,4-Triazine; Antiproliferative activity; Apoptosis; Breast cancer; Piperazine amide; XTT method