title: Human amniotic membrane-derived stromal cells (hAMSC) interact depending on breast cancer cell type through secreted molecules.
abstract: Human amniotic membrane-derived stromal cells (hAMSC) are candidates for cell-based therapies. We examined the characteristics of hAMSC including the interaction between hAMSC and breast cancer cells, MCF-7, and MDA-MB-231. Human amniotic membrane-derived stromal cells showed typical MSC properties, including fibroblast-like morphology, surface antigen expression, and mesodermal differentiation. To investigate cell-cell interaction via secreted molecules, we cultured breast cancer cells in hAMSC-conditioned medium (hAMSC-CM) and analyzed their proliferation, migration, and secretome profiles. MCF-7 and MDA-MB-231 cells exposed to hAMSC-CM showed increased proliferation and migration. However, in hAMSC-CM, MCF-7 cells proliferated significantly faster than MDA-MB-231 cells. When cultured in hAMSC-CM, MCF-7 cells migrated faster than MDA-MB-231 cells. Two cell types showed different profiles of secreted factors. MCF-7 cells expressed much amounts of IL-8, GRO, and MCP-1 in hAMSC-CM. Human amniotic membrane-derived stromal cells interact with breast cancer cells through secreted molecules. Factors secreted by hAMSCs promote the proliferation and migration of MCF-7 breast cancer cells. For much safe cell-based therapies using hAMSC, it is necessary to study carefully about interaction between hAMSC and cancer cells.
authors.affiliation: National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety, Cheongju, Chuncheongbuk-do 363-700, Republic of Korea; Laboratory of Biopharmaceutical Process, College of Life Sciences and Biotechnology, Korea University, Seoul 136-713, Republic of Korea. Electronic address: norway111@korea.kr.
authors.forename: Sun-Hee
authors.initials: SH
authors.lastname: Kim
authors.affiliation: National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety, Cheongju, Chuncheongbuk-do 363-700, Republic of Korea.
authors.forename: So Hee
authors.initials: SH
authors.lastname: Bang
authors.affiliation: National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety, Cheongju, Chuncheongbuk-do 363-700, Republic of Korea.
authors.forename: So Yeong
authors.initials: SY
authors.lastname: Kang
authors.affiliation: National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety, Cheongju, Chuncheongbuk-do 363-700, Republic of Korea.
authors.forename: Ki Dae
authors.initials: KD
authors.lastname: Park
authors.affiliation: National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety, Cheongju, Chuncheongbuk-do 363-700, Republic of Korea.
authors.forename: Jun Ho
authors.initials: JH
authors.lastname: Eom
authors.affiliation: National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety, Cheongju, Chuncheongbuk-do 363-700, Republic of Korea.
authors.forename: Il Ung
authors.initials: IU
authors.lastname: Oh
authors.affiliation: National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety, Cheongju, Chuncheongbuk-do 363-700, Republic of Korea.
authors.forename: Si Hyung
authors.initials: SH
authors.lastname: Yoo
authors.affiliation: Laboratory of Biopharmaceutical Process, College of Life Sciences and Biotechnology, Korea University, Seoul 136-713, Republic of Korea.
authors.forename: Chan-Wha
authors.initials: CW
authors.lastname: Kim
authors.affiliation: National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety, Cheongju, Chuncheongbuk-do 363-700, Republic of Korea.
authors.forename: Sun Young
authors.initials: SY
authors.lastname: Baek
mesh_terms: D000650:Amnion / Q000166:cytology* / Q000378:metabolism; D001943:Breast Neoplasms / Q000235:genetics* / Q000473:pathology; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics*; D064987:Cell- and Tissue-Based Therapy / Q000009:adverse effects*; D018932:Chemokine CCL2 / Q000096:biosynthesis; D054360:Chemokine CXCL1 / Q000096:biosynthesis; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D016209:Interleukin-8 / Q000096:biosynthesis; D061986:MCF-7 Cells; D017154:Stromal Cells
chemical_list: C111835:CCL2 protein, human; C475829:CXCL1 protein, human; D018932:Chemokine CCL2; D054360:Chemokine CXCL1; D016209:Interleukin-8
keywords: Amniotic membrane; MCF-7; MDA-MB-231; Secretion