title: Longitudinal monitoring adipose-derived stem cell survival by PET imaging hexadecyl-4-¹²⁴I-iodobenzoate in rat myocardial infarction model.
abstract: This study aims to monitor how the change of cell survival of transplanted adipose-derived stem cells (ADSCs) responds to myocardial infarction (MI) via the hexadecyl-4-(124)I-iodobenzoate ((124)I-HIB) mediated direct labeling method in vivo. Stem cells have shown the potential to improve cardiac function after MI. However, monitoring of the fate of transplanted stem cells at target sites is still unclear. Rat ADSCs were labeled with (124)I-HIB, and radiolabeled ADSCs were transplanted into the myocardium of normal and MI model. In the group of (124)I-HIB-labeled ADSC transplantation, in vivo imaging was performed using small-animal positron emission tomography (PET)/computed tomography (CT) for 9 days. Twenty-one days post-transplantation, histopathological analysis and apoptosis assay were performed. ADSC viability and differentiation were not affected by (124)I-HIB labeling. In vivo tracking of the (124)I-HIB-labeled ADSCs was possible for 9 and 3 days in normal and MI model, respectively. Apoptosis of transplanted cells increased in the MI model compared than that in normal model. We developed a direct labeling agent, (124)I-HIB, and first tried to longitudinally monitor transplanted stem cell to MI. This approach may provide new insights on the roles of stem cell monitoring in living bodies for stem cell therapy from pre-clinical studies to clinical trials.
authors.affiliation: Molecular Imaging Research Center, Korea Institute of Radiological and Medical Sciences, Seoul, Republic of Korea; School of Life Sciences and Biotechnology, Korea University, Seoul, Republic of Korea.
authors.forename: Min Hwan
authors.initials: MH
authors.lastname: Kim
authors.affiliation: Molecular Imaging Research Center, Korea Institute of Radiological and Medical Sciences, Seoul, Republic of Korea.
authors.forename: Sang-Keun
authors.initials: SK
authors.lastname: Woo
authors.affiliation: Molecular Imaging Research Center, Korea Institute of Radiological and Medical Sciences, Seoul, Republic of Korea.
authors.forename: Kyo Chul
authors.initials: KC
authors.lastname: Lee
authors.affiliation: Molecular Imaging Research Center, Korea Institute of Radiological and Medical Sciences, Seoul, Republic of Korea.
authors.forename: Gwang Il
authors.initials: GI
authors.lastname: An
authors.affiliation: Department of Molecular Medicine, BK21 Plus KNU Biomedical Convergence Program, Kyungpook National University, Daegu, Republic of Korea.
authors.forename: Darpan
authors.initials: D
authors.lastname: Pandya
authors.affiliation: College of Veterinary Medicine, Konkuk University, Seoul, Republic of Korea.
authors.forename: Noh Won
authors.initials: NW
authors.lastname: Park
authors.affiliation: College of Veterinary Medicine, Konkuk University, Seoul, Republic of Korea.
authors.forename: Sang-Soep
authors.initials: SS
authors.lastname: Nahm
authors.affiliation: College of Veterinary Medicine, Konkuk University, Seoul, Republic of Korea.
authors.forename: Ki Dong
authors.initials: KD
authors.lastname: Eom
authors.affiliation: Molecular Imaging Research Center, Korea Institute of Radiological and Medical Sciences, Seoul, Republic of Korea.
authors.forename: Kwang Il
authors.initials: KI
authors.lastname: Kim
authors.affiliation: Molecular Imaging Research Center, Korea Institute of Radiological and Medical Sciences, Seoul, Republic of Korea.
authors.forename: Tae Sup
authors.initials: TS
authors.lastname: Lee
authors.affiliation: School of Life Sciences and Biotechnology, Korea University, Seoul, Republic of Korea.
authors.forename: Chan Wha
authors.initials: CW
authors.lastname: Kim
authors.affiliation: Molecular Imaging Research Center, Korea Institute of Radiological and Medical Sciences, Seoul, Republic of Korea.
authors.forename: Joo Hyun
authors.initials: JH
authors.lastname: Kang
authors.affiliation: Department of Molecular Medicine, BK21 Plus KNU Biomedical Convergence Program, Kyungpook National University, Daegu, Republic of Korea. Electronic address: yooj@knu.ac.kr.
authors.forename: Jeongsoo
authors.initials: J
authors.lastname: Yoo
authors.affiliation: Molecular Imaging Research Center, Korea Institute of Radiological and Medical Sciences, Seoul, Republic of Korea. Electronic address: yjlee@kirams.re.kr.
authors.forename: Yong Jin
authors.initials: YJ
authors.lastname: Lee
mesh_terms: D017667:Adipocytes / Q000166:cytology; D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D017209:Apoptosis; D002470:Cell Survival; D017690:Cell Transplantation; D004195:Disease Models, Animal; D019788:Fluorodeoxyglucose F18 / Q000737:chemistry; D007457:Iodine Radioisotopes / Q000737:chemistry*; D007463:Iodobenzoates / Q000737:chemistry*; D008297:Male; D009203:Myocardial Infarction / Q000000981:diagnostic imaging* / Q000628:therapy*; D009206:Myocardium / Q000378:metabolism; D049268:Positron-Emission Tomography; D019275:Radiopharmaceuticals / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction; D033581:Stem Cell Transplantation; D013234:Stem Cells / Q000166:cytology / Q000000981:diagnostic imaging; D014057:Tomography, X-Ray Computed
chemical_list: D007457:Iodine Radioisotopes; D007463:Iodobenzoates; D019275:Radiopharmaceuticals; D019788:Fluorodeoxyglucose F18
keywords: Adipose-derived stem cells; Cell tracking; Direct labeling agent; Hexadecyl-4-(124)I-iodobenzoate; Myocardial infarction