title: Independent stem cell lineages regulate adipose organogenesis and adipose homeostasis.
abstract: Adipose tissues have striking plasticity, highlighted by childhood and adult obesity. Using adipose lineage analyses, smooth muscle actin (SMA)-mural cell-fate mapping, and conditional PPARγ deletion to block adipocyte differentiation, we find two phases of adipocyte generation that emanate from two independent adipose progenitor compartments: developmental and adult. These two compartments are sequentially required for organ formation and maintenance. Although both developmental and adult progenitors are specified during the developmental period and express PPARγ, they have distinct microanatomical, functional, morphogenetic, and molecular profiles. Furthermore, the two compartments derive from different lineages; whereas adult adipose progenitors fate-map from an SMA+ mural lineage, developmental progenitors do not. Remarkably, the adult progenitor compartment appears to be specified earlier than the developmental cells and then enters the already developmentally formed adipose depots. Thus, two distinct cell compartments control adipose organ development and organ homeostasis, which may provide a discrete therapeutic target for childhood and adult obesity.
authors.affiliation: Department of Developmental Biology, UT Southwestern Medical Center, Dallas, TX 75390-9133, USA.
authors.forename: Yuwei
authors.initials: Y
authors.lastname: Jiang
authors.affiliation: Department of Developmental Biology, UT Southwestern Medical Center, Dallas, TX 75390-9133, USA.
authors.forename: Daniel C
authors.initials: DC
authors.lastname: Berry
authors.affiliation: Department of Developmental Biology, UT Southwestern Medical Center, Dallas, TX 75390-9133, USA.
authors.forename: Wei
authors.initials: W
authors.lastname: Tang
authors.affiliation: Department of Developmental Biology, UT Southwestern Medical Center, Dallas, TX 75390-9133, USA; Department of Molecular Biology, UT Southwestern Medical Center, Dallas, TX 75390-9133, USA. Electronic address: jon.graff@utsouthwestern.edu.
authors.forename: Jonathan M
authors.initials: JM
authors.lastname: Graff
mesh_terms: D000273:Adipose Tissue / Q000166:cytology* / Q000254:growth & development* / Q000378:metabolism; D000375:Aging / Q000378:metabolism; D000818:Animals; D019070:Cell Lineage; D006706:Homeostasis; D051379:Mice; D038081:Organogenesis; D047495:PPAR gamma / Q000378:metabolism; D013194:Staining and Labeling; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism; D013997:Time Factors
chemical_list: D047495:PPAR gamma