title: Urine human papillomavirus prevalence in women with high-grade cervical lesions.
abstract: OBJECTIVE
  To determine the prevalence of human papillomavirus (HPV) in urine samples from women with high-grade cervical lesions. Secondary objectives are to identify the influence of socio-demographic factors and the different genotypes with urinary HPV positivity.
  
  
  METHODS
  75 women with a positive biopsy for CIN2+ were included in the study from October 2010 to July 2011. A sample of urine was collected immediately before conization at the outpatient clinic. We analyzed the presence of HPV using a PCR technique.
  
  
  RESULTS
  The mean age of the patients was 34.8 years (range 24 to 61). All patients had histological CIN2+, of whom 54.67% had CIN3. The prevalence of HPV in urine test was 58.82% in CIN2 population versus 78.05% in CIN3 patients (p 0.072). 31 different genotypes were found. The most frequent HPV genotype was 16-HPV, which was identified in 58% of women with positive HPV-DNA in urine samples. No demographic characteristics were significantly associated to urinary HPV prevalence.
  
  
  CONCLUSIONS
  Most of the patients with CIN2+ showed positive results for urine HPV test. The prevalence of positive urinary HPV test was higher for patients with CIN3. HPV urine detection could be considered as an acceptable option for high-risk population who skip regular screening programs.
authors.affiliation: Obstetrics & Gynecology Department, Hospital del Mar de Barcelona, Parc Salut Mar. Passeig Marítim 25-29, 08003 Barcelona, Spain. Electronic address: paubatalla@hotmail.com.
authors.forename: P
authors.initials: P
authors.lastname: Nicolau
authors.affiliation: Obstetrics & Gynecology Department, Hospital del Mar de Barcelona, Parc Salut Mar. Passeig Marítim 25-29, 08003 Barcelona, Spain; Universitat Autònoma de Barcelona, Hospital del Mar de Barcelona, Parc Salut Mar. Passeig Marítim 25-29, 08003 Barcelona, Spain.
authors.forename: G
authors.initials: G
authors.lastname: Mancebo
authors.affiliation: Obstetrics & Gynecology Department, Hospital del Mar de Barcelona, Parc Salut Mar. Passeig Marítim 25-29, 08003 Barcelona, Spain; IMIM Instituto de Investigación Sanitaria, Hospital del Mar de Barcelona, Parc Salut Mar. Passeig Marítim 25-29, 08003 Barcelona, Spain.
authors.forename: S
authors.initials: S
authors.lastname: Agramunt
authors.affiliation: Obstetrics & Gynecology Department, Hospital del Mar de Barcelona, Parc Salut Mar. Passeig Marítim 25-29, 08003 Barcelona, Spain.
authors.forename: J M
authors.initials: JM
authors.lastname: Solé-Sedeño
authors.affiliation: Unit of Molecular Biology, Pathology Department, Hospital del Mar de Barcelona, Parc Salut Mar. Passeig Marítim 25-29, 08003 Barcelona, Spain; IMIM Instituto de Investigación Sanitaria, Hospital del Mar de Barcelona, Parc Salut Mar. Passeig Marítim 25-29, 08003 Barcelona, Spain.
authors.forename: B
authors.initials: B
authors.lastname: Bellosillo
authors.affiliation: Unit of Molecular Biology, Pathology Department, Hospital del Mar de Barcelona, Parc Salut Mar. Passeig Marítim 25-29, 08003 Barcelona, Spain.
authors.forename: M M
authors.initials: MM
authors.lastname: Muset
authors.affiliation: Pathology Department, Hospital del Mar de Barcelona, Parc Salut Mar. Passeig Marítim 25-29, 08003 Barcelona, Spain.
authors.forename: B
authors.initials: B
authors.lastname: Lloveras
authors.affiliation: Pathology Department, Hospital del Mar de Barcelona, Parc Salut Mar. Passeig Marítim 25-29, 08003 Barcelona, Spain; Universitat Autònoma de Barcelona, Hospital del Mar de Barcelona, Parc Salut Mar. Passeig Marítim 25-29, 08003 Barcelona, Spain.
authors.forename: F
authors.initials: F
authors.lastname: Alameda
authors.affiliation: Obstetrics & Gynecology Department, Hospital del Mar de Barcelona, Parc Salut Mar. Passeig Marítim 25-29, 08003 Barcelona, Spain; Universitat Autònoma de Barcelona, Hospital del Mar de Barcelona, Parc Salut Mar. Passeig Marítim 25-29, 08003 Barcelona, Spain.
authors.forename: R
authors.initials: R
authors.lastname: Carreras
mesh_terms: D000328:Adult; D001706:Biopsy; D002584:Cervix Uteri / Q000473:pathology; D015897:Comorbidity; D004279:DNA, Viral / Q000235:genetics / Q000652:urine; D005260:Female; D005838:Genotype; D052162:Human papillomavirus 16 / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D008875:Middle Aged; D060787:Neoplasm Grading; D027383:Papillomaviridae / Q000235:genetics / Q000302:isolation & purification*; D030361:Papillomavirus Infections / Q000453:epidemiology* / Q000652:urine; D015995:Prevalence; D012189:Retrospective Studies; D014556:Urine / Q000821:virology*; D002583:Uterine Cervical Neoplasms / Q000453:epidemiology* / Q000473:pathology / Q000652:urine*; D002578:Uterine Cervical Dysplasia / Q000453:epidemiology* / Q000473:pathology / Q000652:urine*
chemical_list: D004279:DNA, Viral
keywords: Cervical cancer screening; Cervical intraepithelial dysplasia; DNA-HPV PCR; HPV genotypes; Urine HPV detection