title: Loa loa and Onchocerca ochengi miRNAs detected in host circulation.
abstract: A combination of deep-sequencing and bioinformatics analysis enabled identification of twenty-two microRNA candidates of potential nematode origin in plasma from Loa loa-infected baboons and a further ten from the plasma of an Onchocerca ochengi-infected cow. The obtained data were compared to results from previous work on miRNA candidates from Dirofilaria immitis and O. volvulus found in host circulating blood, to examine the species specificity of the released miRNA. None of the miRNA candidates was found to be present in all four host-parasite scenarios and most of them were specific to only one of them. Eight candidate miRNAs were found to be identical in the full sequence in at least two different infections, while nine candidate miRNAs were found to be similar but not identical in at least four filarial species.
authors.affiliation: Institute of Parasitology, Centre for Host-Parasite Interactions, McGill University, 21,111 Lakeshore Road, Sainte-Anne-de-Bellevue, Quebec H9X 3V9, Canada.
authors.forename: Lucienne
authors.initials: L
authors.lastname: Tritten
authors.affiliation: Institute of Parasitology, Centre for Host-Parasite Interactions, McGill University, 21,111 Lakeshore Road, Sainte-Anne-de-Bellevue, Quebec H9X 3V9, Canada.
authors.forename: Maeghan
authors.initials: M
authors.lastname: O'Neill
authors.affiliation: Department of Biological Sciences, Western Michigan University, Kalamazoo, Michigan 49008, USA.
authors.forename: Chuck
authors.initials: C
authors.lastname: Nutting
authors.affiliation: Parasite and Vector Research Unit, Department of Microbiology and Parasitology, University of Buea, P.O. Box, 63, Cameroon.
authors.forename: Samuel
authors.initials: S
authors.lastname: Wanji
authors.affiliation: Parasite and Vector Research Unit, Department of Microbiology and Parasitology, University of Buea, P.O. Box, 63, Cameroon.
authors.forename: Abdel
authors.initials: A
authors.lastname: Njouendoui
authors.affiliation: Parasite and Vector Research Unit, Department of Microbiology and Parasitology, University of Buea, P.O. Box, 63, Cameroon.
authors.forename: Fanny
authors.initials: F
authors.lastname: Fombad
authors.affiliation: Parasite and Vector Research Unit, Department of Microbiology and Parasitology, University of Buea, P.O. Box, 63, Cameroon.
authors.forename: Jonas
authors.initials: J
authors.lastname: Kengne-Ouaffo
authors.affiliation: Department of Pathobiology and Diagnostic Investigation, College of Veterinary Medicine, Michigan State University, East Lansing, Michigan 48824, USA.
authors.forename: Charles
authors.initials: C
authors.lastname: Mackenzie
authors.affiliation: Institute of Parasitology, Centre for Host-Parasite Interactions, McGill University, 21,111 Lakeshore Road, Sainte-Anne-de-Bellevue, Quebec H9X 3V9, Canada. Electronic address: timothy.g.geary@mcgill.ca.
authors.forename: Timothy
authors.initials: T
authors.lastname: Geary
mesh_terms: D000818:Animals; D001483:Base Sequence; D002417:Cattle; D002418:Cattle Diseases / Q000097:blood* / Q000469:parasitology; D008117:Loa / Q000235:genetics* / Q000378:metabolism; D008118:Loiasis / Q000097:blood / Q000469:parasitology / Q000662:veterinary*; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D009854:Onchocerca / Q000235:genetics* / Q000378:metabolism; D009855:Onchocerciasis / Q000097:blood / Q000469:parasitology / Q000662:veterinary*; D010215:Papio / Q000097:blood / Q000469:parasitology; D018419:Primate Diseases / Q000097:blood* / Q000469:parasitology; D018510:RNA, Helminth / Q000235:genetics* / Q000378:metabolism
chemical_list: D035683:MicroRNAs; D018510:RNA, Helminth
keywords: Blood; Circulating microRNA; Filarial nematodes; Host–parasite interactions; Loa loa; Onchocerca ochengi