title: Phosphatase activity and culture conditions of the yeast Candida mycoderma sp. and analysis of organic phosphorus hydrolysis ability.
abstract: Orthophosphate is an essential but limiting macronutrient for plant growth. About 67% cropland in China lacks sufficient phosphorus, especially that with red soil. Extensive soil phosphorus reserves exist in the form of organic phosphorus, which is unavailable for root uptake unless hydrolyzed by secretory acid phosphatases. Thus, many microorganisms with the ability to produce phosphatase have been exploited. In this work, the activity of an extracellular acid phosphatase and yeast biomass from Candida mycoderma was measured under different culture conditions, such as pH, temperature, and carbon source. A maximal phosphatase activity of 8.47×10(5)±0.11×10(5)U/g was achieved by C. Mycoderma in 36 hr under the optimal conditions. The extracellular acid phosphatase has high activity over a wide pH tolerance range from 2.5 to 5.0 (optimum pH3.5). The effects of different phosphorus compounds on the acid phosphatase production were also studied. The presence of phytin, lecithin or calcium phosphate reduced the phosphatase activity and biomass yield significantly. In addition, the pH of the culture medium was reduced significantly by lecithin. The efficiency of the strain in releasing orthophosphate from organic phosphorus was studied in red soil (used in planting trees) and rice soil (originating as red soil). The available phosphorus content was increased by 230% after inoculating 20 days in rice soil and decreased by 50% after inoculating 10 days in red soil. This work indicates that the yeast strain C. mycoderma has potential application for enhancing phosphorus utilization in plants that grow in rice soil.
authors.affiliation: Jiangsu Engineering and Technology Research Centre for Microbiology Resource, Jiangsu Key Laboratory for Biodiversity and Biotechnology, Jiangsu Key Laboratory for Microbes and Functional Genomic, College of Life Science, Nanjing Normal University, Nanjing 210023, China. Electronic address: yanmang1987@163.com.
authors.forename: Mang
authors.initials: M
authors.lastname: Yan
authors.affiliation: School of Biotechnology and Chemical Engineering, Taizhou College, Nanjing Normal University, Taizhou 225300, China.
authors.forename: Liufang
authors.initials: L
authors.lastname: Yu
authors.affiliation: School of Biotechnology and Chemical Engineering, Taizhou College, Nanjing Normal University, Taizhou 225300, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Jiangsu Engineering and Technology Research Centre for Microbiology Resource, Jiangsu Key Laboratory for Biodiversity and Biotechnology, Jiangsu Key Laboratory for Microbes and Functional Genomic, College of Life Science, Nanjing Normal University, Nanjing 210023, China.
authors.forename: Yuexia
authors.initials: Y
authors.lastname: Guo
authors.affiliation: College of Geographical Sciences, Nanjing Normal University, Nanjing 210023, China.
authors.forename: Kewei
authors.initials: K
authors.lastname: Dai
authors.affiliation: Jiangsu Engineering and Technology Research Centre for Microbiology Resource, Jiangsu Key Laboratory for Biodiversity and Biotechnology, Jiangsu Key Laboratory for Microbes and Functional Genomic, College of Life Science, Nanjing Normal University, Nanjing 210023, China. Electronic address: chenyuru@njnu.edu.cn.
authors.forename: Yuru
authors.initials: Y
authors.lastname: Chen
mesh_terms: D018533:Biomass; D002175:Candida / Q000201:enzymology / Q000378:metabolism*; D003470:Culture Media; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D010744:Phosphoric Monoester Hydrolases / Q000378:metabolism*; D010758:Phosphorus / Q000378:metabolism*
chemical_list: D003470:Culture Media; D010758:Phosphorus; D010744:Phosphoric Monoester Hydrolases
keywords: Acid phosphatase; Available phosphorus; Candida mycoderma; Lecithin; Organic phosphorus compounds