title: Calcium release-activated calcium (CRAC) channels mediate the β(2)-adrenergic regulation of Na,K-ATPase.
abstract: β2-Adrenergic agonists have been shown to regulate Na,K-ATPase in the alveolar epithelium by recruiting Na,K-ATPase-containing vesicles to the plasma membrane of alveolar epithelial cells (AEC). Here, we provide evidence that β2-agonists induce store-operated calcium entry (SOCE) in AECs. This calcium entry is necessary for β2-agonist-induced recruitment of Na,K-ATPase to the plasma membrane of AECs. Specifically, we show that β2-agonists induce SOCE via stromal interaction molecule 1 (STIM1)-associated calcium release-activated calcium (CRAC) channels. We also demonstrate that the magnitude of SOCE affects the abundance of Na,K-ATPase at the plasma membrane of AECs.
authors.affiliation: Division of Pulmonary and Critical Care Medicine, Department of Medicine, Northwestern University, Feinberg School of Medicine, Chicago, IL 60611, USA.
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Keller
authors.affiliation: Division of Pulmonary and Critical Care Medicine, Department of Medicine, Northwestern University, Feinberg School of Medicine, Chicago, IL 60611, USA. Electronic address: e-lecuona@northwestern.edu.
authors.forename: Emilia
authors.initials: E
authors.lastname: Lecuona
authors.affiliation: Department of Molecular Pharmacology and Biological Chemistry, Northwestern University, Feinberg School of Medicine, Chicago, IL 60611, USA.
authors.forename: Murali
authors.initials: M
authors.lastname: Prakriya
authors.affiliation: Division of Pulmonary and Critical Care Medicine, Department of Medicine, Northwestern University, Feinberg School of Medicine, Chicago, IL 60611, USA.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Cheng
authors.affiliation: Division of Pulmonary and Critical Care Medicine, Department of Medicine, Northwestern University, Feinberg School of Medicine, Chicago, IL 60611, USA.
authors.forename: Saul
authors.initials: S
authors.lastname: Soberanes
authors.affiliation: Division of Pulmonary and Critical Care Medicine, Department of Medicine, Northwestern University, Feinberg School of Medicine, Chicago, IL 60611, USA.
authors.forename: G R Scott
authors.initials: GR
authors.lastname: Budinger
authors.affiliation: Division of Pulmonary and Critical Care Medicine, Department of Medicine, Northwestern University, Feinberg School of Medicine, Chicago, IL 60611, USA.
authors.forename: Jacob I
authors.initials: JI
authors.lastname: Sznajder
mesh_terms: D000262:Adenylyl Cyclases / Q000378:metabolism; D058672:Adrenergic beta-2 Receptor Antagonists / Q000494:pharmacology*; D000420:Albuterol / Q000494:pharmacology; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002135:Calcium-Binding Proteins / Q000378:metabolism*; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D004789:Enzyme Activation / Q000187:drug effects; D004847:Epithelial Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D042541:Intracellular Space / Q000187:drug effects / Q000378:metabolism; D008297:Male; D008562:Membrane Glycoproteins / Q000378:metabolism; D021381:Protein Transport / Q000187:drug effects; D011650:Pulmonary Alveoli / Q000166:cytology; D051381:Rats; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism*; D000071737:Stromal Interaction Molecule 1
chemical_list: D058672:Adrenergic beta-2 Receptor Antagonists; D002135:Calcium-Binding Proteins; D008562:Membrane Glycoproteins; C518857:Stim1 protein, rat; D000071737:Stromal Interaction Molecule 1; D000262:Adenylyl Cyclases; D000254:Sodium-Potassium-Exchanging ATPase; D000420:Albuterol; D002118:Calcium
keywords: Calcium channel; Calcium signaling; Epithelial cell; Na,K-ATPase; Store-operated calcium entry