title: Muscle enzyme and fiber type-specific sarcomere protein increases in serum after inertial concentric-eccentric exercise.
abstract: Muscle damage induced by inertial exercise performed on a flywheel device was assessed through the serum evolution of muscle enzymes, interleukin 6, and fiber type-specific sarcomere proteins such as fast myosin (FM) and slow myosin (SM). We hypothesized that a model of muscle damage could be constructed by measuring the evolution of serum concentration of muscle proteins following inertial exercise, according to their molecular weight and the fiber compartment in which they are located. Moreover, by measuring FM and SM, the type of fibers that are affected could be assessed. Serum profiles were registered before and 24, 48, and 144 h after exercise in 10 healthy and recreationally active young men. Creatine kinase (CK) and CK-myocardial band isoenzyme increased in serum early (24 h) and returned to baseline values after 48 h. FM increased in serum late (48 h) and remained elevated 144 h post-exercise. The increase in serum muscle enzymes suggests increased membrane permeability of both fast and slow fibers, and the increase in FM reveals sarcomere disruption as well as increased membrane permeability of fast fibers. Consequently, FM could be adopted as a fiber type-specific biomarker of muscle damage.
authors.affiliation: Institut Nacional d'Educació Física de Catalunya (INEFC), University of Barcelona, Barcelona, Spain.
authors.forename: G
authors.initials: G
authors.lastname: Carmona
authors.affiliation: Departament de Ciències Fisiològiques I, University of Barcelona, Barcelona, Spain.
authors.forename: M
authors.initials: M
authors.lastname: Guerrero
authors.affiliation: Departament de Ciències Fisiològiques I, University of Barcelona, Barcelona, Spain.
authors.forename: R
authors.initials: R
authors.lastname: Cussó
authors.affiliation: Institut Nacional d'Educació Física de Catalunya (INEFC), University of Barcelona, Barcelona, Spain.
authors.forename: J M
authors.initials: JM
authors.lastname: Padullés
authors.affiliation: Institut Nacional d'Educació Física de Catalunya (INEFC), University of Barcelona, Barcelona, Spain.
authors.forename: G
authors.initials: G
authors.lastname: Moras
authors.affiliation: Institut Nacional d'Educació Física de Catalunya (INEFC), University of Barcelona, Barcelona, Spain.
authors.forename: M
authors.initials: M
authors.lastname: Lloret
authors.affiliation: Hospital Clínic de Barcelona, Barcelona, Spain.
authors.forename: J L
authors.initials: JL
authors.lastname: Bedini
authors.affiliation: Institut Nacional d'Educació Física de Catalunya (INEFC), University of Barcelona, Barcelona, Spain.|Departament de Ciències Fisiològiques I, University of Barcelona, Barcelona, Spain.
authors.forename: J A
authors.initials: JA
authors.lastname: Cadefau
mesh_terms: D000328:Adult; D000410:Alanine Transaminase / Q000097:blood; D001219:Aspartate Aminotransferases / Q000097:blood; D052279:Creatine Kinase, MB Form / Q000097:blood; D015444:Exercise / Q000502:physiology*; D005080:Exercise Test / Q000295:instrumentation; D006801:Humans; D015850:Interleukin-6 / Q000097:blood*; D008297:Male; D009119:Muscle Contraction / Q000502:physiology; D018485:Muscle Fibers, Skeletal / Q000201:enzymology* / Q000473:pathology; D053580:Muscle Strength; D063806:Myalgia / Q000175:diagnosis; D010147:Pain Measurement; D012518:Sarcomeres / Q000378:metabolism; D024744:Skeletal Muscle Myosins / Q000097:blood*; D013997:Time Factors; D055815:Young Adult
chemical_list: D015850:Interleukin-6; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D052279:Creatine Kinase, MB Form; D024744:Skeletal Muscle Myosins
keywords: Inertial exercise; creatine kinase; dynamic force-generating capacity; exercise-induced muscle damage; fast myosin; knee extensor