title: Infection of human amniotic and endothelial cells by Japanese encephalitis virus: Increased expression of HLA-F.
abstract: Productive infection of human amniotic and endothelial cell lines with Japanese encephalitis virus (JEV) was established leading to the induction of NFκB and HLA-F, a non-classical MHC molecule. Induction of the HLA-F gene and protein in JEV-infected cells was shown to be NFκB dependent since it was blocked by inhibitors of NFκB activation. ShRNA targeting lentivirus-mediated stable knockdown of the p65 subunit of NFκB inhibited JEV-mediated induction of HLA-F both in the amniotic cell line, AV-3 as well as the human brain microendothelial cell line, HBMEC. The induction of HLA-F by treatment of AV-3 with TNF-α was also inhibited by ShRNA mediated knockdown of NFκB. TNF-α treatment of HEK293T cells that were transfected with reporter plasmids under the control of HLA-F enhancer A elements resulted in significant transactivation of the luciferase reporter gene. NFκB-mediated induction of HLA-F following JEV infection and TNF-α exposure is being suggested for the first time.
authors.affiliation: Department of Biochemistry, Indian Institute of Science, Bangalore 560012, India. Electronic address: gaurav@biochem.iisc.ernet.in.
authors.forename: Gaurav
authors.initials: G
authors.lastname: Kumar
authors.affiliation: Department of Biochemistry, Indian Institute of Science, Bangalore 560012, India. Electronic address: date.onkar@gmail.com.
authors.forename: Onkar Sanjay
authors.initials: OS
authors.lastname: Date
authors.affiliation: Department of pediatric infectious diseases, John Hopkins university school of medicine, Baltimore, MD 21287, USA. Electronic address: kwangkim@jhmi.edu.
authors.forename: Kwang Sik
authors.initials: KS
authors.lastname: Kim
authors.affiliation: Department of Biochemistry, Indian Institute of Science, Bangalore 560012, India. Electronic address: mjnhm@biochem.iisc.ernet.in.
authors.forename: Ramanathapuram
authors.initials: R
authors.lastname: Manjunath
mesh_terms: D000653:Amniotic Fluid / Q000166:cytology* / Q000187:drug effects / Q000821:virology; D000818:Animals; D002460:Cell Line; D004664:Encephalitis Virus, Japanese / Q000502:physiology*; D042783:Endothelial Cells / Q000821:virology*; D015967:Gene Expression Regulation, Viral; D015395:Histocompatibility Antigens Class I / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D016328:NF-kappa B / Q000037:antagonists & inhibitors / Q000378:metabolism; D011817:Rabbits; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
chemical_list: C062341:HLA-F antigens; D015395:Histocompatibility Antigens Class I; D016328:NF-kappa B; D014409:Tumor Necrosis Factor-alpha
keywords: Amniotic; Endothelial cells; JEV; NFκB mediated HLA-F induction; TNF-α