title: Myoepithelial cells from pleomorphic adenoma are not influenced by tumor conditioned media from breast ductal adenocarcinoma and melanoma cells: Anin vitrostudy.
abstract: Myoepithelial cells have been implicated in the regulation of the transition fromin situto invasive neoplasia in salivary gland tumors. Considering the importance of the microenvironment of the tumor, the presentin vitrostudy therefore analyzed the morphological and phenotypic changes undergone by benign myoepithelial cells from pleomorphic adenoma (PA) stimulated by tumor-conditioned medium. The benign myoepithelial cells were obtained from PA and were cultured with fibronectin extracellular matrix protein, supplemented with tumor-conditioned medium, which was harvested from breast ductal adenocarcinoma AU-565 and melanoma Hs 852.T cells. The morphological alterations were assessed by immunofluorescence analysis using vimentin antibody. The α-smooth muscle actin (α-SMA) and fibroblast growth factor (FGF)-2 proteins were analyzed by indirect immunofluorescence and quantitative polymerase chain reaction (qPCR). No morphological changes were observed in the myoepithelial cells cultured in fibronectin protein under stimulation from either tumor-conditioned medium. The immunofluorescence results, which were supported by qPCR analysis, revealed that only α-SMA was upregulated in the fibronectin substratum, with or without tumor-conditioned medium obtained from breast ductal adenocarcinoma and melanoma cells. No significant difference in FGF-2 mRNA expression was detected when the cells were cultured either in the tumor-conditioned medium or in the fibronectin substratum. The tumor-conditioned medium harvested from breast ductal adenocarcinoma and melanoma did not affect myoepithelial cell differentiation and function, which was reflected by the fact that there was no observed increase in α-SMA and FGF-2 expression, respectively.
authors.affiliation: Department of Oral Pathology, São Leopoldo Mandic Institute and Research Center, Campinas, São Paulo 13045-610, Brazil.
authors.forename: Elizabeth Ferreira
authors.initials: EF
authors.lastname: Martinez
authors.affiliation: Department of Oral Pathology, São Leopoldo Mandic Institute and Research Center, Campinas, São Paulo 13045-610, Brazil.
authors.forename: Ana Paula Dias
authors.initials: AP
authors.lastname: Demasi
authors.affiliation: Department of Oral Pathology, São Leopoldo Mandic Institute and Research Center, Campinas, São Paulo 13045-610, Brazil.
authors.forename: Marcelo Henrique
authors.initials: MH
authors.lastname: Napimoga
authors.affiliation: Department of Oral Pathology, São Leopoldo Mandic Institute and Research Center, Campinas, São Paulo 13045-610, Brazil.
authors.forename: Carolina Amália Barcellos
authors.initials: CA
authors.lastname: Silva
authors.affiliation: Department of Oral Pathology, São Leopoldo Mandic Institute and Research Center, Campinas, São Paulo 13045-610, Brazil.
authors.forename: Natalia Festugatto
authors.initials: NF
authors.lastname: Navarini
authors.affiliation: Department of Oral Pathology, São Leopoldo Mandic Institute and Research Center, Campinas, São Paulo 13045-610, Brazil.
authors.forename: Ney Soares
authors.initials: NS
authors.lastname: Araújo
authors.affiliation: Department of Oral Pathology, São Leopoldo Mandic Institute and Research Center, Campinas, São Paulo 13045-610, Brazil.
authors.forename: Vera Cavalcanti
authors.initials: VC
authors.lastname: DE Araújo
keywords: malignant cell lines; myoepithelial cells; salivary gland tumors; tumor microenvironment