title: Proteomic and transcriptomic analysis of rice tranglutaminase and chloroplast-related proteins.
abstract: The recently cloned rice transglutaminase gene (tgo) is the second plant transglutaminase identified to date (Campos et al. Plant Sci. 205-206 (2013) 97-110). Similarly to its counterpart in maize (tgz), this rice TGase was localized in the chloroplast, although in this case not exclusively. To further characterise plastidial tgo functionality, proteomic and transcriptomic studies were carried out to identify possible TGO-related proteins. Some LHCII antenna proteins were identified as TGO related using an in vitro proteomic approach, as well as ATPase and some PSII core proteins by mass spectrometry. To study the relationship between TGO and other plastidial proteins, a transcriptomic in vivo Dynamic Array (Fluidigm™) was used to analyse the mRNA expression of 30 plastidial genes with respect to that of tgo, in rice plants subjected to different periods of continuous illumination. The results indicated a gene-dependent tendency in the expression pattern that was related to tgo expression and to the illumination cycle. For certain genes, including tgo, significant differences between treatments, principally at the initiation and/or at the end of the illumination period, connected with the day/night cycling of gene expression, were observed. The tgo expression was especially related to plastidial proteins involved in photoprotection and the thylakoid electrochemical gradient.
authors.affiliation: Molecular Genetics Department, Centre for Research in Agricultural Genomics: CRAG (Consorci CSIC-IRTA-UAB-UB), Campus UAB, Bellaterra, 08193 Barcelona, Spain. Electronic address: nefer@costarricense.cr.
authors.forename: N
authors.initials: N
authors.lastname: Campos
authors.affiliation: Molecular Genetics Department, Centre for Research in Agricultural Genomics: CRAG (Consorci CSIC-IRTA-UAB-UB), Campus UAB, Bellaterra, 08193 Barcelona, Spain. Electronic address: josepmaria.torne@cragenomica.es.
authors.forename: J M
authors.initials: JM
authors.lastname: Torné
authors.affiliation: Institute of Advanced Chemistry of Catalonia IQAC, CSIC, Jordi Girona 18-26, 08034 Barcelona, Spain. Electronic address: mjose.bleda@iqac.csic.es.
authors.forename: M J
authors.initials: MJ
authors.lastname: Bleda
authors.affiliation: Department of Chemical and Surfactants Technology, Institute of Advanced Chemistry of Catalonia IQAC, CSIC, Jordi Girona 18-26, 08034 Barcelona, Spain. Electronic address: albert.manich@iqac.csic.es.
authors.forename: A
authors.initials: A
authors.lastname: Manich
authors.affiliation: Biotechnology Department, Neiker-Tecnalia, Arkaute, Vitoria, Campus Agroalimentario de Arkaute, Aptdo.46, E-01080 Vitoria-Gasteiz, Araba, Spain. Electronic address: iurreta@neiker.es.
authors.forename: I
authors.initials: I
authors.lastname: Urreta
authors.affiliation: Biotechnology Department, Neiker-Tecnalia, Arkaute, Campus Agroalimentario de Arkaute Apto 46, E-01080, Vitoria-Gasteiz, Araba, Spain. Electronic address: imontalban@neiker.net.
authors.forename: I A
authors.initials: IA
authors.lastname: Montalbán
authors.affiliation: Biotechnology Department, Neiker-Tecnalia, Arkaute, Campus Agroalimentario de Arkaute, Aptdo.46, E-01080 Vitoria-Gasteiz, Araba, Spain. Electronic address: scdelatorre@neiker.net.
authors.forename: S
authors.initials: S
authors.lastname: Castañón
authors.affiliation: Biotechnology Department, Neiker-Tecnalia, Arkaute, Campus Agroalimentario de Arkaute, Apto 46, E-01080 Vitoria-Gasteiz, Araba, Spain. Electronic address: pmoncalean@neiker.net.
authors.forename: P
authors.initials: P
authors.lastname: Moncalean
authors.affiliation: Molecular Genetics Department, Centre for Research in Agricultural Genomics: CRAG (Consorci CSIC-IRTA-UAB-UB), Campus UAB, Bellaterra, 08193 Barcelona, Spain. Electronic address: mireia.santos@iqac.csic.es.
authors.forename: M
authors.initials: M
authors.lastname: Santos
mesh_terms: D060365:Chloroplast Proteins / Q000235:genetics / Q000378:metabolism*; D002853:Chromatography, Liquid; D015180:Electrophoresis, Gel, Two-Dimensional; D005453:Fluorescence; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant / Q000528:radiation effects; D008027:Light; D013058:Mass Spectrometry; D012275:Oryza / Q000201:enzymology* / Q000235:genetics* / Q000528:radiation effects; D011485:Protein Binding / Q000528:radiation effects; D020543:Proteome / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020524:Thylakoids / Q000378:metabolism / Q000528:radiation effects; D059467:Transcriptome / Q000235:genetics*
chemical_list: D060365:Chloroplast Proteins; D020543:Proteome; D012333:RNA, Messenger
keywords: Dynamic array; Light dependence; Photosynthesis-related proteins; Relative mRNA gene expression; Rice; Transglutaminase