title: Comparison of two point of care devices for capillary lipid screening in fasting and postprandial adults.
abstract: OBJECTIVE
  The aim of this study was to assess the performance of two point of care (POC) devices for capillary lipid screening in fasting and post-prandial adults.
  
  
  METHODS
  Fasting and post-prandial capillary whole blood samples collected from 57 adult donors were analyzed simultaneously on Cholestech LDX Lipid Profile (Alere San Diego, Inc., San Diego, CA) cassettes and CardioChek Lipid Panel (Polymer Technology Systems, Indianapolis, IN) strips. Paired serum samples were collected from the same donors and analyzed with CDC-certified methods for total cholesterol, high density lipoprotein cholesterol (HDL-C) and non-blanked triglycerides. Non-HDL-C (total cholesterol minus HDL-C) and low density lipoprotein cholesterol (LDL-C) were calculated. Mean bias between capillary whole blood and serum laboratory lipids was calculated.
  
  
  RESULTS
  HDL-C measurements were not affected by triglyceride content on either device. However, both devices exhibited significant variability in triglyceride measurement relative to the reference method. Compared to reference methods, Cholestech was more accurate than CardioChek for non-HDL-C while CardioChek was more accurate for HDL-C. Among the calculated cardiovascular risk parameters (LDL-C and non-HDL-C), Cholestech-calculated non-HDL-C exhibited the least average bias in both fasting and postprandial samples.
  
  
  CONCLUSIONS
  The optimal approach to capillary lipid screening may be to use Cholestech non-HDL cholesterol; as it exhibited little bias relative to CDC reference methods in both fasting and postprandial samples, facilitating lipid screening in non-fasting adults.
authors.affiliation: Department of Laboratory Medicine & Pathology, Mayo Clinic, Rochester, MN 55905, USA. Electronic address: donato.leslie@mayo.edu.
authors.forename: Leslie J
authors.initials: LJ
authors.lastname: Donato
authors.affiliation: Department of Laboratory Medicine & Pathology, Mayo Clinic, Rochester, MN 55905, USA.
authors.forename: Gayle R
authors.initials: GR
authors.lastname: Deobald
authors.affiliation: Department of Laboratory Medicine & Pathology, Mayo Clinic, Rochester, MN 55905, USA.
authors.forename: Amy M
authors.initials: AM
authors.lastname: Wockenfus
authors.affiliation: Department of Laboratory Medicine & Pathology, Mayo Clinic, Rochester, MN 55905, USA.
authors.forename: Jean M
authors.initials: JM
authors.lastname: Hornseth
authors.affiliation: Department of Laboratory Medicine & Pathology, Mayo Clinic, Rochester, MN 55905, USA.
authors.forename: Amy K
authors.initials: AK
authors.lastname: Saenger
authors.affiliation: Department of Laboratory Medicine & Pathology, Mayo Clinic, Rochester, MN 55905, USA.
authors.forename: Brad S
authors.initials: BS
authors.lastname: Karon
mesh_terms: D000328:Adult; D002196:Capillaries / Q000378:metabolism*; D002784:Cholesterol / Q000097:blood; D005215:Fasting / Q000097:blood*; D006801:Humans; D008055:Lipids / Q000097:blood*; D008403:Mass Screening / Q000295:instrumentation*; D019095:Point-of-Care Systems; D019518:Postprandial Period; D012016:Reference Values
chemical_list: D008055:Lipids; D002784:Cholesterol
keywords: Lipid screening; Non-HDL-C; Point-of-care lipids