title: Protein release from electrospun nonwovens: improving the release characteristics through rational combination of polyester blend matrices with polidocanol.
abstract: Nonwoven scaffolds consisting of poly-ε-caprolactone (PCL), poly(lactic-co-glycolic acid) (PLGA) and polidocanol (PD), and loaded with lysozyme crystals were prepared by electrospinning. The composition of the matrix was varied and the effect of PD content in binary mixtures, and of PD and PLGA content in ternary mixtures regarding processability, fiber morphology, water sorption, swelling and drug release was investigated. Binary PCL/PD blend nonwovens showed a PD-dependent increase in swelling of up to 30% and of lysozyme burst release of up to 45% associated with changes of the fiber morphology. Furthermore, addition of free PD to the release medium resulted in a significant increase of lysozyme burst release from pure PCL nonwovens from approximately 2-35%. Using ternary PCL/PD/PLGA blends, matrix degradation could be significantly improved over PCL/PD blends, resulting in a biphasic release of lysozyme with constant release over 9 weeks, followed by constant release with a reduced rate over additional 4 weeks. Based on these results, protein release from PCL scaffolds is improved by blending with PD due to improved lysozyme desorption from the polymer surface and PD-dependent matrix swelling.
authors.affiliation: Institute for Pharmacy and Food Chemistry, University of Wuerzburg, Am Hubland, Wuerzburg 97074 , Germany.
authors.forename: Sebastian
authors.initials: S
authors.lastname: Puhl
authors.affiliation: Institute for Pharmacy and Food Chemistry, University of Wuerzburg, Am Hubland, Wuerzburg 97074 , Germany.
authors.forename: David
authors.initials: D
authors.lastname: Ilko
authors.affiliation: Institute for Pharmacy and Food Chemistry, University of Wuerzburg, Am Hubland, Wuerzburg 97074 , Germany; Key Laboratory of Biorheological Science and Technology, Ministry of Education, College of Bioengineering, Chongqing University, Chongqing 400030, PR China.
authors.forename: Linhao
authors.initials: L
authors.lastname: Li
authors.affiliation: Institute for Pharmacy and Food Chemistry, University of Wuerzburg, Am Hubland, Wuerzburg 97074 , Germany.
authors.forename: Ulrike
authors.initials: U
authors.lastname: Holzgrabe
authors.affiliation: Institute for Pharmacy and Food Chemistry, University of Wuerzburg, Am Hubland, Wuerzburg 97074 , Germany.
authors.forename: Lorenz
authors.initials: L
authors.lastname: Meinel
authors.affiliation: Institute for Pharmacy and Food Chemistry, University of Wuerzburg, Am Hubland, Wuerzburg 97074 , Germany; Institute for Pharma Technology, University of Applied Sciences Northwestern Switzerland, Gruendenstrasse 40, Muttenz 4132, Switzerland. Electronic address: oliver.germershaus@fhnw.ch.
authors.forename: Oliver
authors.initials: O
authors.lastname: Germershaus
mesh_terms: D002152:Calorimetry, Differential Scanning; D003460:Crystallization; D004337:Drug Carriers / Q000737:chemistry*; D004339:Drug Compounding; D065546:Drug Liberation; D019344:Lactic Acid / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D009113:Muramidase / Q000008:administration & dosage* / Q000737:chemistry; D010316:Particle Size; D000077423:Polidocanol; D011091:Polyesters / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry*; D011100:Polyglycolic Acid / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D013499:Surface Properties
chemical_list: D004337:Drug Carriers; D011091:Polyesters; D000077423:Polidocanol; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; C016240:polycaprolactone; D011100:Polyglycolic Acid; D019344:Lactic Acid; D011092:Polyethylene Glycols; D009113:Muramidase
keywords: Electrospinning; Lysozyme; Polidocanol; Polycaprolactone; Protein release