title: RNA aptamer based electrochemical biosensor for sensitive and selective detection of cAMP.
abstract: Cyclic adenosine monophosphate (cAMP) is an important small biological molecule associated with the healthy state of living organism. In order to realize highly sensitive and specific detection of cAMP, here an RNA aptamer and electrochemical impedance spectroscopy (EIS) based biosensor enhanced by gold nanoparticles electrodeposited on the surface of gold electrode is designed. The designed aptasensor has a wide effective measuring range from 50pM to 250pM with a detection limit of 50pM in PBS buffer, and an effective measuring range from 50nM to 1μM with a detection limit of 50nM in serum. The designed biosensor is also able to detect cAMP with high sensitivity, specificity, and stability. Since the biosensor can be easily fabricated with low cost and repeatedly used for at least two times, it owns great potential in wide application fields such as clinical test and food inspection, etc.
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China.
authors.forename: Fulin
authors.initials: F
authors.lastname: Zhao
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China.
authors.forename: Qingyun
authors.initials: Q
authors.lastname: Xie
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China.
authors.forename: Mingfei
authors.initials: M
authors.lastname: Xu
authors.affiliation: Department of Information Physics and Engineering, Nanjing University of Science and Technology, Nanjing, Jiangsu 210094, China.
authors.forename: Shouyu
authors.initials: S
authors.lastname: Wang
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China.
authors.forename: Jiyong
authors.initials: J
authors.lastname: Zhou
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China. Electronic address: feiliu24@njau.edu.cn.
authors.forename: Fei
authors.initials: F
authors.lastname: Liu
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry*; D015374:Biosensing Techniques / Q000379:methods*; D000242:Cyclic AMP / Q000302:isolation & purification*; D058266:Dielectric Spectroscopy; D006046:Gold / Q000737:chemistry; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry
chemical_list: D052157:Aptamers, Nucleotide; D006046:Gold; D000242:Cyclic AMP
keywords: Cyclic adenosine monophosphate; Electrochemical impedance spectroscopy; Gold nanoparticles; RNA aptamer