title: Measurement of the biophysical properties of porcine adipose-derived stem cells by a microperfusion system.
abstract: Adipose-derived stem cells (ADSCs), which are an accessible source of adult stem cells with capacities for self-renewal and differentiation into various cell types, have a promising potential in tissue engineering and regenerative medicine strategies. To meet the clinical demand for ADSCs, cryopreservation has been applied for long-term ADSC preservation. To optimize the addition, removal, freezing, and thawing of cryoprotective agents (CPAs) applied to ADSCs, we measured the transport properties of porcine ADSCs (pADSCs). The cell responses of pADSCs to hypertonic phosphate-buffered saline and common CPAs, dimethyl sulfoxide, ethylene glycol, and glycerol were measured by a microperfusion system at temperatures of 28, 18, 8, and -2°C. We determined the osmotically inactive cell volume (Vb), hydraulic conductivity (Lp), and CPA permeability (Ps) at various temperatures in a two-parameter model. Then, we quantitatively analyzed the effect of temperature on the transport properties of the pADSC membrane. Biophysical parameters were used to optimize CPA addition, removal, and freezing processes to minimize excessive shrinkage of pADSCs during cryopreservation. The biophysical properties of pADSCs have a great potential for effective optimization of cryopreservation procedures.
authors.affiliation: Centre for Biomedical Engineering, Department of Electronic Science and Technology, University of Science and Technology of China, Hefei, Anhui 230027, China.
authors.forename: Jianye
authors.initials: J
authors.lastname: Wang
authors.affiliation: Centre for Biomedical Engineering, Department of Electronic Science and Technology, University of Science and Technology of China, Hefei, Anhui 230027, China; Anhui Provincial Engineering Technology Research Center for Biopreservation and Artificial Organs, Hefei, Anhui 230027, China. Electronic address: zhaog@ustc.edu.cn.
authors.forename: Gang
authors.initials: G
authors.lastname: Zhao
authors.affiliation: Anhui Provincial Laboratory for Local Livestock and Poultry Genetic Resource Conservation and Bio-breeding, College of Animal Science and Technology, Anhui Agricultural University, Hefei, Anhui 230036, China.
authors.forename: Pengfei
authors.initials: P
authors.lastname: Zhang
authors.affiliation: Centre for Biomedical Engineering, Department of Electronic Science and Technology, University of Science and Technology of China, Hefei, Anhui 230027, China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Anhui Provincial Laboratory for Local Livestock and Poultry Genetic Resource Conservation and Bio-breeding, College of Animal Science and Technology, Anhui Agricultural University, Hefei, Anhui 230036, China. Electronic address: yunhaizhang@ahau.edu.cn.
authors.forename: Yunhai
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Centre for Biomedical Engineering, Department of Electronic Science and Technology, University of Science and Technology of China, Hefei, Anhui 230027, China; Anhui Provincial Engineering Technology Research Center for Biopreservation and Artificial Organs, Hefei, Anhui 230027, China.
authors.forename: Dayong
authors.initials: D
authors.lastname: Gao
authors.affiliation: Anhui Provincial Engineering Technology Research Center for Biopreservation and Artificial Organs, Hefei, Anhui 230027, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Zhou
authors.affiliation: Anhui Provincial Engineering Technology Research Center for Biopreservation and Artificial Organs, Hefei, Anhui 230027, China.
authors.forename: Yunxia
authors.initials: Y
authors.lastname: Cao
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D002463:Cell Membrane Permeability; D002478:Cells, Cultured; D015925:Cryopreservation / Q000295:instrumentation*; D003451:Cryoprotective Agents / Q000378:metabolism*; D004867:Equipment Design; D005615:Freezing; D006801:Humans; D008853:Microscopy / Q000295:instrumentation; D008954:Models, Biological; D009997:Osmotic Pressure; D010477:Perfusion / Q000295:instrumentation*; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism; D013552:Swine
chemical_list: D003451:Cryoprotective Agents
keywords: Adipose-derived stem cell; Biophysical properties; Cryopreservation; Microperfusion system