title: Anaerobic mineralization of 2,4,6-tribromophenol to CO2 by a synthetic microbial community comprising Clostridium, Dehalobacter, and Desulfatiglans.
abstract: Anaerobic mineralization of 2,4,6-tribromophenol (2,4,6-TBP) was achieved by a synthetic anaerobe community comprising a highly enriched culture of Dehalobacter sp. phylotype FTH1 acting as a reductive debrominator; Clostridium sp. strain Ma13 acting as a hydrogen supplier via glucose fermentation; and a novel 4-chlorophenol-degrading anaerobe, Desulfatiglans parachlorophenolica strain DS. 2,4,6-TBP was debrominated to phenol by the combined action of Ma13 and FTH1, then mineralized into CO2 by sequential introduction of DS, confirmed using [ring-(14)C(U)] phenol. The optimum concentrations of glucose, SO4(2-), and inoculum densities were 0.5 or 2.5mM, 1.0 or 2.5mM, and the densities equivalent to 10(4)copiesmL(-1) of the 16S rRNA genes, respectively. This resulted in the complete mineralization of 23μM 2,4,6-TBP within 35days (0.58μmolL(-1)d(-1)). Thus, using a synthetic microbial community of isolates or highly enriched cultures would be an efficient, optimizable, low-cost strategy for anaerobic bioremediation of halogenated aromatics.
authors.affiliation: State Key Laboratory of Urban Water Resources and Environment, Harbin Institute of Technology (SKLUWRE, HIT), Harbin 150090, China; EcoTopia Science Institute, Nagoya University, Chikusa, Nagoya 464-8603, Japan.
authors.forename: Zhiling
authors.initials: Z
authors.lastname: Li
authors.affiliation: Center for Fostering Young and Innovative Researchers, Nagoya Institute of Technology, Syowa, Nagoya 466-8555, Japan.
authors.forename: Naoko
authors.initials: N
authors.lastname: Yoshida
authors.affiliation: State Key Laboratory of Urban Water Resources and Environment, Harbin Institute of Technology (SKLUWRE, HIT), Harbin 150090, China; Key Laboratory of Environmental Biotechnology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China.
authors.forename: Aijie
authors.initials: A
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Urban Water Resources and Environment, Harbin Institute of Technology (SKLUWRE, HIT), Harbin 150090, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Nan
authors.affiliation: Key Laboratory of Environmental Biotechnology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Liang
authors.affiliation: EcoTopia Science Institute, Nagoya University, Chikusa, Nagoya 464-8603, Japan.
authors.forename: Chunfang
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Graduate School of Engineering, Nagoya University, Chikusa, Nagoya 464-8603, Japan.
authors.forename: Dongdong
authors.initials: D
authors.lastname: Zhang
authors.affiliation: EcoTopia Science Institute, Nagoya University, Chikusa, Nagoya 464-8603, Japan.
authors.forename: Daisuke
authors.initials: D
authors.lastname: Suzuki
authors.affiliation: Graduate School of Environmental Studies, Nagoya University, Nagoya 464-8601, Japan.
authors.forename: Xue
authors.initials: X
authors.lastname: Zhou
authors.affiliation: Graduate School of Engineering, Nagoya University, Chikusa, Nagoya 464-8603, Japan.
authors.forename: Zhixing
authors.initials: Z
authors.lastname: Xiao
authors.affiliation: EcoTopia Science Institute, Nagoya University, Chikusa, Nagoya 464-8603, Japan; Graduate School of Engineering, Nagoya University, Chikusa, Nagoya 464-8603, Japan; Graduate School of Environmental Studies, Nagoya University, Nagoya 464-8601, Japan. Electronic address: a-katayama@esi.nagoya-u.ac.jp.
authors.forename: Arata
authors.initials: A
authors.lastname: Katayama
mesh_terms: D000693:Anaerobiosis; D061249:Batch Cell Culture Techniques; D001673:Biodegradation, Environmental; D002245:Carbon Dioxide / Q000378:metabolism*; D003013:Clostridium / Q000378:metabolism*; D020564:Deltaproteobacteria / Q000378:metabolism*; D052918:Environmental Restoration and Remediation / Q000379:methods; D007700:Kinetics; D010459:Peptococcaceae / Q000378:metabolism*; D010636:Phenols / Q000378:metabolism*
chemical_list: D010636:Phenols; D002245:Carbon Dioxide; C004554:2,4,6-tribromophenol
keywords: 2,4,6-Tribromophenol; Anaerobic mineralization; Synthetic anaerobe community; The optimized conditions