title: Enhancing microalgal biomass productivity by engineering a microalgal-bacterial community.
abstract: This study demonstrates that ecologically engineered bacterial consortium could enhance microalgal biomass and lipid productivities through carbon exchange. Phycosphere bacterial diversity analysis in xenic Chlorella vulgaris (XCV) confirmed the presence of growth enhancing and inhibiting microorganisms. Co-cultivation of axenic C. vulgaris (ACV) with four different growth enhancing bacteria revealed a symbiotic relationship with each bacterium. An artificial microalgal-bacterial consortium (AMBC) constituting these four bacteria and ACV showed that the bacterial consortium exerted a statistically significant (P<0.05) growth enhancement on ACV. Moreover, AMBC had superior flocculation efficiency, lipid content and quality. Studies on carbon exchange revealed that bacteria in AMBC might utilize fixed organic carbon released by microalgae, and in return, supply inorganic and low molecular weight (LMW) organic carbon influencing algal growth and metabolism. Such exchanges, although species specific, have enormous significance in carbon cycle and can be exploitated by microalgal biotechnology industry.
authors.affiliation: Sustainable Bioresource Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 305 806, Republic of Korea.
authors.forename: Dae-Hyun
authors.initials: DH
authors.lastname: Cho
authors.affiliation: Sustainable Bioresource Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 305 806, Republic of Korea.
authors.forename: Rishiram
authors.initials: R
authors.lastname: Ramanan
authors.affiliation: Sustainable Bioresource Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 305 806, Republic of Korea; Major of Green Chemistry and Environmental Biotechnology, University of Science & Technology (UST), Daejeon 305 350, Republic of Korea.
authors.forename: Jina
authors.initials: J
authors.lastname: Heo
authors.affiliation: Sustainable Bioresource Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 305 806, Republic of Korea.
authors.forename: Jimin
authors.initials: J
authors.lastname: Lee
authors.affiliation: Sustainable Bioresource Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 305 806, Republic of Korea.
authors.forename: Byung-Hyuk
authors.initials: BH
authors.lastname: Kim
authors.affiliation: Sustainable Bioresource Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 305 806, Republic of Korea; Major of Green Chemistry and Environmental Biotechnology, University of Science & Technology (UST), Daejeon 305 350, Republic of Korea.
authors.forename: Hee-Mock
authors.initials: HM
authors.lastname: Oh
authors.affiliation: Sustainable Bioresource Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 305 806, Republic of Korea; Major of Green Chemistry and Environmental Biotechnology, University of Science & Technology (UST), Daejeon 305 350, Republic of Korea. Electronic address: hkim@kribb.re.kr.
authors.forename: Hee-Sik
authors.initials: HS
authors.lastname: Kim
mesh_terms: D001419:Bacteria / Q000235:genetics / Q000378:metabolism; D018533:Biomass; D001709:Biotechnology / Q000379:methods*; D002244:Carbon / Q000378:metabolism; D048408:Chlorella vulgaris / Q000254:growth & development / Q000378:metabolism / Q000382:microbiology*; D005427:Flocculation; D050356:Lipid Metabolism; D008055:Lipids / Q000737:chemistry; D058086:Microalgae / Q000254:growth & development / Q000378:metabolism / Q000382:microbiology*; D059013:Microbial Consortia / Q000235:genetics / Q000502:physiology*; D013559:Symbiosis
chemical_list: D008055:Lipids; D002244:Carbon
keywords: Artificial microalgal bacterial community; Biodiesel; Chlorella vulgaris; Growth enhancement; Phycosphere bacteria