title: Bioremediation potential of microorganisms derived from petroleum reservoirs.
abstract: Bacterial strains and metagenomic clones, both obtained from petroleum reservoirs, were evaluated for petroleum degradation abilities either individually or in pools using seawater microcosms for 21 days. Gas Chromatography-Flame Ionization Detector (GC-FID) and Gas Chromatography-Mass Spectrometry (GC-MS) analyses were carried out to evaluate crude oil degradation. The results showed that metagenomic clones 1A and 2B were able to biodegrade n-alkanes (C14 to C33) and isoprenoids (phytane and pristane), with rates ranging from 31% to 47%, respectively. The bacteria Dietzia maris CBMAI 705 and Micrococcus sp. CBMAI 636 showed higher rates reaching 99% after 21 days. The metagenomic clone pool biodegraded these compounds at rates ranging from 11% to 45%. Regarding aromatic compound biodegradation, metagenomic clones 2B and 10A were able to biodegrade up to 94% of phenanthrene and methylphenanthrenes (3-MP, 2-MP, 9-MP and 1-MP) with rates ranging from 55% to 70% after 21 days, while the bacteria Dietzia maris CBMAI 705 and Micrococcus sp. CBMAI 636 were able to biodegrade 63% and up to 99% of phenanthrene, respectively, and methylphenanthrenes (3-MP, 2-MP, 9-MP and 1-MP) with rates ranging from 23% to 99% after 21 days. In this work, isolated strains as well as metagenomic clones were capable of degrading several petroleum compounds, revealing an innovative strategy and a great potential for further biotechnological and bioremediation applications.
authors.affiliation: Microbial Resources Division, Research Center for Chemistry, Biology and Agriculture (CPQBA), University of Campinas - UNICAMP, CEP 13148-218, Paulinia, Brazil.
authors.forename: Bruna Martins
authors.initials: BM
authors.lastname: Dellagnezze
authors.affiliation: Laboratory of Engineering and Petroleum Exploration, Darcy Ribeiro North Fluminense State University - LENEP/UENF, POB 119562, 27910-970 Macaé, RJ, Brazil.
authors.forename: Gabriel Vasconcelos
authors.initials: GV
authors.lastname: de Sousa
authors.affiliation: Laboratory of Engineering and Petroleum Exploration, Darcy Ribeiro North Fluminense State University - LENEP/UENF, POB 119562, 27910-970 Macaé, RJ, Brazil.
authors.forename: Laercio Lopes
authors.initials: LL
authors.lastname: Martins
authors.affiliation: Microbial Resources Division, Research Center for Chemistry, Biology and Agriculture (CPQBA), University of Campinas - UNICAMP, CEP 13148-218, Paulinia, Brazil.
authors.forename: Daniela Ferreira
authors.initials: DF
authors.lastname: Domingos
authors.affiliation: Microbial Resources Division, Research Center for Chemistry, Biology and Agriculture (CPQBA), University of Campinas - UNICAMP, CEP 13148-218, Paulinia, Brazil.
authors.forename: Elmer E G
authors.initials: EEG
authors.lastname: Limache
authors.affiliation: Federal University of São Paulo - UNIFESP, CEP 04021-001, São Paulo, Brazil.
authors.forename: Suzan Pantaroto
authors.initials: SP
authors.lastname: de Vasconcellos
authors.affiliation: Laboratory of Engineering and Petroleum Exploration, Darcy Ribeiro North Fluminense State University - LENEP/UENF, POB 119562, 27910-970 Macaé, RJ, Brazil.
authors.forename: Georgiana Feitosa
authors.initials: GF
authors.lastname: da Cruz
authors.affiliation: Microbial Resources Division, Research Center for Chemistry, Biology and Agriculture (CPQBA), University of Campinas - UNICAMP, CEP 13148-218, Paulinia, Brazil. Electronic address: vmaia@cpqba.unicamp.br.
authors.forename: Valéria Maia
authors.initials: VM
authors.lastname: de Oliveira
mesh_terms: D000473:Alkanes / Q000378:metabolism; D001419:Bacteria / Q000235:genetics / Q000378:metabolism; D001673:Biodegradation, Environmental; D001938:Brazil; D002849:Chromatography, Gas; D042926:Cytochrome P-450 CYP4A / Q000235:genetics; D008401:Gas Chromatography-Mass Spectrometry; D006841:Hydrocarbons, Aromatic / Q000378:metabolism; D059013:Microbial Consortia / Q000235:genetics / Q000502:physiology*; D008837:Micrococcus / Q000378:metabolism; D061329:Oil and Gas Fields / Q000382:microbiology*; D010578:Petroleum / Q000032:analysis / Q000378:metabolism*; D010616:Phenanthrenes / Q000378:metabolism; D012623:Seawater / Q000382:microbiology
chemical_list: D000473:Alkanes; D006841:Hydrocarbons, Aromatic; D010578:Petroleum; D010616:Phenanthrenes; C031181:phenanthrene; D042926:Cytochrome P-450 CYP4A
keywords: Bioremediation; Metagenomic clones; Petroleum biodegradation; Seawater