title: Electrophoresis of periodontal pathogens in poly(ethyleneoxide) solutions with uncoated capillary.
abstract: Periodontitis is a prevalent inflammatory disease caused by different species of anaerobic bacteria such as Porphyromonas gingivalis (P.g), Treponema denticola (T.d), and Tannerella forsythia (T.f). We compared the separation result of DNA ladders in hydroxyethyl cellulose, poly(ethyleneoxide) (PEO), and polyethylene glycol and analyzed the effect of polymer concentration, electric field, and temperature of the background electrolyte on the separation performance. Results demonstrated that there was a linear relationship (R=0.942) for 100 to 700bp of DNA and its migration time. Finally, the polymerase chain reaction products of P.g, T.d, and T.f were successfully identified within 8.5 min in 0.5% PEO with uncoated capillary.
authors.affiliation: Engineering Research Center of Optical Instrument and System, Ministry of Education, Shanghai Key Laboratory of Modern Optical System, University of Shanghai for Science and Technology, Shanghai 200093, China. Electronic address: zhenqingli@163.com.
authors.forename: Zhenqing
authors.initials: Z
authors.lastname: Li
authors.affiliation: Engineering Research Center of Optical Instrument and System, Ministry of Education, Shanghai Key Laboratory of Modern Optical System, University of Shanghai for Science and Technology, Shanghai 200093, China.
authors.forename: Ronglin
authors.initials: R
authors.lastname: Yang
authors.affiliation: Engineering Research Center of Optical Instrument and System, Ministry of Education, Shanghai Key Laboratory of Modern Optical System, University of Shanghai for Science and Technology, Shanghai 200093, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Engineering Research Center of Optical Instrument and System, Ministry of Education, Shanghai Key Laboratory of Modern Optical System, University of Shanghai for Science and Technology, Shanghai 200093, China.
authors.forename: Dawei
authors.initials: D
authors.lastname: Zhang
authors.affiliation: Engineering Research Center of Optical Instrument and System, Ministry of Education, Shanghai Key Laboratory of Modern Optical System, University of Shanghai for Science and Technology, Shanghai 200093, China.
authors.forename: Songlin
authors.initials: S
authors.lastname: Zhuang
authors.affiliation: Photonics and Biomedical Research Institute, Department of Physics, Faculty of Science, East China University of Science and Technology, Shanghai 200237, China; Department of Applied Physics, Graduate School of Engineering, Osaka University, Suita City, Osaka 565-0871, Japan. Electronic address: yoshi.yamaguchi@ap.eng.osaka-u.ac.jp.
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Yamaguchi
mesh_terms: D001419:Bacteria / Q000235:genetics / Q000302:isolation & purification*; D002482:Cellulose / Q000737:chemistry; D004269:DNA, Bacterial / Q000302:isolation & purification; D019075:Electrophoresis, Capillary / Q000379:methods*; D010518:Periodontitis / Q000382:microbiology*; D020959:Polyethylene / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry*; D012996:Solutions; D013696:Temperature
chemical_list: D004269:DNA, Bacterial; D012996:Solutions; D011092:Polyethylene Glycols; D020959:Polyethylene; D002482:Cellulose
keywords: Capillary electrophoresis; Periodontal pathogens; Polymerase chain reaction