title: Neonatal sex and weight influence CD34(+) cell concentration in umbilical cord blood but not stromal cell-derived factor 1-3'A polymorphism.
abstract: BACKGROUND
  Umbilical cord blood (UCB) has been used as an alternative source of donor hematopoietic stem cells for hematologic transplant setting over the past decade. This study attempted to evaluate potential predictors of cord blood quality.
  
  
  METHODS
  A total of 750 UCB samples were studied (male, n = 365; female, n = 385). The impact of neonatal sex, weight and stromal cell-derived factor-1α polymorphism on the quality of these UCB samples was investigated.
  
  
  RESULTS
  Male neonatal UCB was significantly richer in CD34(+) cells than was female UCB (P < 0.001), whereas female UCB was richer in total nucleated cells (P = 0.01). There was a slight correlation between CD34(+) cells concentration and UCB sample weight (P < 0.01) that could be attributed to the higher weight of male neonates. The use of tetra-polymerase chain reaction to detect stromal cell-derived factor-1α polymorphisms in 180 neonates revealed no differences between A/A, G/G and A/G allelic combinations.
  
  
  CONCLUSIONS
  These data emphasize the lack of predictive factors for CD34(+) cells and total nucleated cell concentrations in UCB samples before processing.
authors.affiliation: Etablissement Français du Sang Aquitaine-Limousin, Bordeaux, France. Electronic address: f.bijou@bordeaux.unicancer.fr.
authors.forename: Fontanet
authors.initials: F
authors.lastname: Bijou
authors.affiliation: Etablissement Français du Sang Aquitaine-Limousin, Bordeaux, France.
authors.forename: Zoran
authors.initials: Z
authors.lastname: Ivanovic
authors.affiliation: Etablissement Français du Sang Aquitaine-Limousin, Bordeaux, France.
authors.forename: Dominique
authors.initials: D
authors.lastname: Fizet
authors.affiliation: Etablissement Français du Sang Aquitaine-Limousin, Bordeaux, France.
authors.forename: Bernard
authors.initials: B
authors.lastname: Dazey
authors.affiliation: Etablissement Français du Sang Aquitaine-Limousin, Bordeaux, France.
authors.forename: Jean-Michel
authors.initials: JM
authors.lastname: Boiron
authors.affiliation: Etablissement Français du Sang Aquitaine-Limousin, Bordeaux, France.
authors.forename: Xavier
authors.initials: X
authors.lastname: Lafarge
mesh_terms: D018952:Antigens, CD34 / Q000032:analysis*; D001724:Birth Weight / Q000502:physiology*; D000094902:Blood Banking / Q000379:methods; D054377:Chemokine CXCL12 / Q000235:genetics*; D036101:Cord Blood Stem Cell Transplantation / Q000379:methods; D005260:Female; D005312:Fetal Blood / Q000166:cytology*; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006801:Humans; D007231:Infant, Newborn; D008297:Male; D011110:Polymorphism, Genetic; D012727:Sex Characteristics
chemical_list: D018952:Antigens, CD34; C515255:CXCL12 protein, human; D054377:Chemokine CXCL12
keywords: hematopoietic stem cell; stem cell source; stem cell transplantation; umbilical cord blood