title: Investigation on antidiarrhoeal activity of Aristolochia indica Linn. Root extracts in mice.
abstract: BACKGROUND
  The present study aimed at investigating the effect of ethanolic extract (EtAI), and aqueous extract (AqAI) of Aristolochia indica Linn roots on castor oil-induced diarrhoea and study on small intestinal transit. Phytochemical analysis of extracts was performed as per standard procedure.
  
  
  METHODS
  The oral toxicity study using Swiss albino mice was performed in accordance with OECD guidelines. The EtAI and AqAI extracts of Aristolochia indica Linn were studied for antidiarrhoeal property using castor oil-induced diarrhoeal model and charcoal-induced gastrointestinal motility test in Swiss albino mice.
  
  
  RESULTS
  Among the tested doses of 200 and 400 mg/kg body weight, the extracts reduced the frequency and severity of diarrhoea in test animals throughout the study period. At the same doses, the extract delayed the intestinal transit of charcoal meal in test animals as compared to the control and the results were statistically significant.
  
  
  CONCLUSIONS
  Experimental findings showed that ethanol extract of Aristolochia indica Linn root possess significant antidiarrheal activity and may be a potent source of anti-diarrhoeal drug in future.
authors.affiliation: School of Pharmacy, International Medical University, Bukit Jalil, Kuala Lumpur, Malaysia.
authors.forename: Senthil Rajan
authors.initials: SR
authors.lastname: Dharmalingam
authors.affiliation: Department of Pharmacognosy, Padmavathi College of Pharmacy and Research Institute, India.
authors.forename: Rajkumar
authors.initials: R
authors.lastname: Madhappan
authors.affiliation: School of Pharmacy, International Medical University, Bukit Jalil, Kuala Lumpur, Malaysia.
authors.forename: Srinivasan
authors.initials: S
authors.lastname: Ramamurthy
authors.affiliation: School of Pharmacy, International Medical University, Bukit Jalil, Kuala Lumpur, Malaysia.
authors.forename: Kumarappan
authors.initials: K
authors.lastname: Chidambaram
authors.affiliation: School of Pharmacy, International Medical University, Bukit Jalil, Kuala Lumpur, Malaysia.
authors.forename: Meka Venkata
authors.initials: MV
authors.lastname: Srikanth
authors.affiliation: School of Pharmacy, International Medical University, Bukit Jalil, Kuala Lumpur, Malaysia.
authors.forename: Suresh
authors.initials: S
authors.lastname: Shanmugham
authors.affiliation: Department of Pharmacognosy, Padmavathi College of Pharmacy and Research Institute, India.
authors.forename: K L
authors.initials: KL
authors.lastname: Senthil Kumar
mesh_terms: D000818:Animals; D000930:Antidiarrheals / Q000008:administration & dosage*; D030022:Aristolochia / Q000737:chemistry*; D003967:Diarrhea / Q000188:drug therapy*; D006801:Humans; D008297:Male; D051379:Mice; D010936:Plant Extracts / Q000008:administration & dosage*; D018517:Plant Roots / Q000737:chemistry
chemical_list: D000930:Antidiarrheals; D010936:Plant Extracts
keywords: Antidiarrheal activity; Aristolochia indica Linn; ethanol extract; small intestinal transit