title: Enzymatic hydrolysis of steam-exploded sugarcane bagasse using high total solids and low enzyme loadings.
abstract: Hydrolysis of phosphoric acid-impregnated steam-treated sugarcane bagasse was pre-optimized using a face-centered central composite design in which the process variables were the substrate total solids (TS, %), agitation intensity (AI, rpm) and enzyme loading (EL, gg(-1)). Pretreatment was carried out at 180°C for 10min using cane bagasse with 50wt% moisture content containing 9.5mg of H3PO4 per gram of dry biomass. Hydrolyses were performed for 96h at 50°C using Cellic CTec2® and water-washed steam-treated substrates. The highest amount of fermentable sugars was obtained with 20wt% TS, producing 76.8gL(-1) of glucose equivalents, which corresponded to a total glucan conversion of 69.2wt% and to a theoretical net increase of 39% in ethanol production from the same sugarcane tonnage without considering the use of leaves, tops and the additional yields from C5 sugars.
authors.affiliation: Research Center in Applied Chemistry (CEPESQ), Department of Chemistry, Federal University of Paraná, Curitiba, PR, Brazil; INCT in Energy & Environment, Department of Chemistry, Federal University of Paraná, Brazil. Electronic address: luiz.ramos@ufpr.br.
authors.forename: Luiz Pereira
authors.initials: LP
authors.lastname: Ramos
authors.affiliation: Research Center in Applied Chemistry (CEPESQ), Department of Chemistry, Federal University of Paraná, Curitiba, PR, Brazil.
authors.forename: Larissa
authors.initials: L
authors.lastname: da Silva
authors.affiliation: Research Center in Applied Chemistry (CEPESQ), Department of Chemistry, Federal University of Paraná, Curitiba, PR, Brazil.
authors.forename: Annielly Comelli
authors.initials: AC
authors.lastname: Ballem
authors.affiliation: Research Center in Applied Chemistry (CEPESQ), Department of Chemistry, Federal University of Paraná, Curitiba, PR, Brazil.
authors.forename: Ana Paula
authors.initials: AP
authors.lastname: Pitarelo
authors.affiliation: Research Center in Applied Chemistry (CEPESQ), Department of Chemistry, Federal University of Paraná, Curitiba, PR, Brazil.
authors.forename: Luana Marcele
authors.initials: LM
authors.lastname: Chiarello
authors.affiliation: Research Center in Applied Chemistry (CEPESQ), Department of Chemistry, Federal University of Paraná, Curitiba, PR, Brazil.
authors.forename: Marcos Henrique Luciano
authors.initials: MH
authors.lastname: Silveira
mesh_terms: D018533:Biomass; D001709:Biotechnology / Q000379:methods*; D002241:Carbohydrates; D002482:Cellulose / Q000737:chemistry / Q000378:metabolism; D004798:Enzymes / Q000737:chemistry / Q000378:metabolism*; D005285:Fermentation; D005936:Glucans / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D006868:Hydrolysis; D008962:Models, Theoretical; D031786:Saccharum / Q000737:chemistry* / Q000378:metabolism; D013227:Steam
chemical_list: D002241:Carbohydrates; D004798:Enzymes; D005936:Glucans; D013227:Steam; D002482:Cellulose; C027433:bagasse; D005947:Glucose
keywords: Enzymatic hydrolysis; High total solids; Phosphoric acid; Steam explosion; Sugarcane bagasse