title: Structural characterization and bioactivities of sulfated polysaccharide from Monostroma oxyspermum.
abstract: Sulfated polysaccharide was isolated from Monostroma oxyspermum through hot water extraction, anion-exchange and gel permeation column chromatography. The sulfated polysaccharide contained 92% of carbohydrate, 0% of protein, 7.8% of uronic acid, 22% of ash and 33% of moisture respectively. The elemental composition was analyzed using CHNS/O analyzer. The molecular weight of sulfated polysaccharide determined through PAGE was found to be as 55 kDa. Monosaccharides analysis revealed that sulfated polysaccharide was composed of rhamnose, fructose, galactose, xylose, and glucose. The structural features of sulfated polysaccharide were analyzed by NMR spectroscopy. Further the sulfated polysaccharide showed total antioxidant and DPPH free radical scavenging activity were as 66.29% at 250 μg/ml and 66.83% at 160 μg/ml respectively. The sulfated polysaccharide also showed ABTS scavenging ability and reducing power were as 83.88% at 125 μg/ml and 15.81% at 400 μg/ml respectively. The anticoagulant activity was determined for human plasma with respect to Activated Partial Thromboplastin Time (APTT) and Prothrombin Time (PT) was 20.09 IU and 1.79 IU at 25 μg/ml respectively. These results indicated that the sulfated polysaccharide from M. oxyspermum had potent antioxidant and anticoagulant activities.
authors.affiliation: Centre of Advanced Study in Marine Biology, Faculty of Marine Sciences, Annamalai University, Parangipettai 608 502, Tamil Nadu, India. Electronic address: seedevisalem@gmail.com.
authors.forename: Palaniappan
authors.initials: P
authors.lastname: Seedevi
authors.affiliation: Centre of Advanced Study in Marine Biology, Faculty of Marine Sciences, Annamalai University, Parangipettai 608 502, Tamil Nadu, India.
authors.forename: Meivelu
authors.initials: M
authors.lastname: Moovendhan
authors.affiliation: Centre of Advanced Study in Marine Biology, Faculty of Marine Sciences, Annamalai University, Parangipettai 608 502, Tamil Nadu, India.
authors.forename: Sadhasivam
authors.initials: S
authors.lastname: Sudharsan
authors.affiliation: Department of Chemical Engineering, Annamalai University, Annamalai Nagar - 608 002, Tamil Nadu, India.
authors.forename: Shanmugam
authors.initials: S
authors.lastname: Vasanthkumar
authors.affiliation: Department of Biophysics, All India Institute of Medical Sciences, New Delhi - 110 029, India.
authors.forename: Alagiri
authors.initials: A
authors.lastname: Srinivasan
authors.affiliation: Centre of Advanced Study in Marine Biology, Faculty of Marine Sciences, Annamalai University, Parangipettai 608 502, Tamil Nadu, India.
authors.forename: Shanmugam
authors.initials: S
authors.lastname: Vairamani
authors.affiliation: Centre of Advanced Study in Marine Biology, Faculty of Marine Sciences, Annamalai University, Parangipettai 608 502, Tamil Nadu, India.
authors.forename: Annaian
authors.initials: A
authors.lastname: Shanmugam
mesh_terms: D000925:Anticoagulants / Q000494:pharmacology; D000975:Antioxidants / Q000494:pharmacology; D052160:Benzothiazoles / Q000737:chemistry; D001713:Biphenyl Compounds / Q000737:chemistry; D000460:Chlorophyta / Q000737:chemistry*; D016166:Free Radical Scavengers / Q000494:pharmacology; D006801:Humans; D008970:Molecular Weight; D009005:Monosaccharides / Q000032:analysis; D010084:Oxidation-Reduction; D010851:Picrates / Q000737:chemistry; D011134:Polysaccharides / Q000737:chemistry* / Q000494:pharmacology*; D066244:Proton Magnetic Resonance Spectroscopy; D017550:Spectroscopy, Fourier Transform Infrared; D013451:Sulfonic Acids / Q000737:chemistry
chemical_list: D000925:Anticoagulants; D000975:Antioxidants; D052160:Benzothiazoles; D001713:Biphenyl Compounds; D016166:Free Radical Scavengers; D009005:Monosaccharides; D010851:Picrates; D011134:Polysaccharides; D013451:Sulfonic Acids; C002502:2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid; C004931:1,1-diphenyl-2-picrylhydrazyl
keywords: Anticoagulant activity; Antioxidant; M. oxyspermum; Sulfated polysaccharide