title: Presence of Viral Genome in Urine and Development of Hematuria and Pathological Changes in Kidneys in Common Marmoset (Callithrix jacchus) after Inoculation with Dengue Virus.
abstract: Common marmosets (Callithrix jacchus) developed high levels of viremia, clinical signs including fever, weight loss, a decrease in activity and hematuria upon inoculation with dengue virus (DENV). Presence of DENV genome in urine samples and pathological changes in kidneys were examined in the present study. Levels of DENV genome were determined in 228 urine samples from 20 primary DENV-inoculated marmosets and in 56 urine samples from four secondary DENV-inoculated marmosets. DENV genome was detected in 75% (15/20) of marmosets after primary DENV infection. No DENV genome was detected in urine samples from the marmosets with secondary infection with homologous DENV (0%, 0/4). Two marmosets demonstrated hematuria. Pathological analysis of the kidneys demonstrated non-suppressive interstitial nephritis with renal tubular regeneration. DENV antigen-positive cells were detected in kidneys. In human dengue virus infections, some patients present renal symptoms. The results indicate that marmosets recapitulate some aspects of the involvement of kidneys in human DENV infection, and suggest that marmosets are potentially useful for the studies of the pathogenesis of DENV infection, including kidneys.
authors.affiliation: National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku-ku, Tokyo, 162-8640, Japan. sherry@nih.go.jp.
authors.forename: Meng Ling
authors.initials: ML
authors.lastname: Moi
authors.affiliation: National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku-ku, Tokyo, 162-8640, Japan. tomatsu@cc.tuat.ac.jp.
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Omatsu
authors.affiliation: National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku-ku, Tokyo, 162-8640, Japan. hirayama@jata.or.jp.
authors.forename: Takanori
authors.initials: T
authors.lastname: Hirayama
authors.affiliation: Shiga University of Medical Science, Seta Tsukinowa-cho, Otsu, Shiga, 520-2192, Japan. snakamur@belle.shiga-med.ac.jp.
authors.forename: Shinichiro
authors.initials: S
authors.lastname: Nakamura
authors.affiliation: National Institute of Biomedical Innovation, 1 Hachimandai, Tsukuba, Ibaraki, 305-0843, Japan. katakai@primate.or.jp.
authors.forename: Yuko
authors.initials: Y
authors.lastname: Katakai
authors.affiliation: Primate Research Institute, Kyoto University, Inuyama, Aichi, 484-8506, Japan. ytomoyuki@pri.kyoto-u.ac.jp.
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Yoshida
authors.affiliation: Primate Research Institute, Kyoto University, Inuyama, Aichi, 484-8506, Japan. saito.akatsuki.6a@kyoto-u.ac.jp.
authors.forename: Akatsuki
authors.initials: A
authors.lastname: Saito
authors.affiliation: National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku-ku, Tokyo, 162-8640, Japan. stajima@nih.go.jp.
authors.forename: Shigeru
authors.initials: S
authors.lastname: Tajima
authors.affiliation: National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku-ku, Tokyo, 162-8640, Japan. mito0908@yahoo.co.jp.
authors.forename: Mikako
authors.initials: M
authors.lastname: Ito
authors.affiliation: National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku-ku, Tokyo, 162-8640, Japan. takasaki@nih.go.jp.
authors.forename: Tomohiko
authors.initials: T
authors.lastname: Takasaki
authors.affiliation: Primate Research Institute, Kyoto University, Inuyama, Aichi, 484-8506, Japan. akari@pri.kyoto-u.ac.jp.
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Akari
authors.affiliation: National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku-ku, Tokyo, 162-8640, Japan. kurane@nih.go.jp.
authors.forename: Ichiro
authors.initials: I
authors.lastname: Kurane