title: Development of a bioautographic method for the detection of lipase inhibitors.
abstract: An autobiographic method based on the thin layer chromatogram was developed by using the chemical system that comprises p-Nitrophenyl butyrate and bromothymol blue for detecting the lipase inhibitor. Lipase inhibitory zones were visualized as blue spots against the greenish yellow background. This method could able to detect the well known lipase inhibitor, orlistat up to the concentration of 1ng which is better than the earlier method. This method could also able to detect the lipase inhibition activities from the un-explored species of Streptomyces. The developed method can be used not only for the screening of unknown samples for the lipase inhibitors but also for the purification of the lipase inhibitors from the unknown samples.
authors.affiliation: Food Science and Technology Division, Centre for Emerging Technologies, Jain University, Jain Global Campus, Jakkasandra, Kanakapura Main Road, Ramanagara Dist., Karnataka 562112, India.
authors.forename: Venkata Krishna
authors.initials: VK
authors.lastname: Bayineni
authors.affiliation: Food Science and Technology Division, Centre for Emerging Technologies, Jain University, Jain Global Campus, Jakkasandra, Kanakapura Main Road, Ramanagara Dist., Karnataka 562112, India.
authors.forename: Sukrutha
authors.initials: S
authors.lastname: Suresh
authors.affiliation: Food Science and Technology Division, Centre for Emerging Technologies, Jain University, Jain Global Campus, Jakkasandra, Kanakapura Main Road, Ramanagara Dist., Karnataka 562112, India.
authors.forename: Gurmeet
authors.initials: G
authors.lastname: Singh
authors.affiliation: Food Science and Technology Division, Centre for Emerging Technologies, Jain University, Jain Global Campus, Jakkasandra, Kanakapura Main Road, Ramanagara Dist., Karnataka 562112, India. Electronic address: k.ravikumar@jainuniversity.ac.in.
authors.forename: Ravi-Kumar
authors.initials: RK
authors.lastname: Kadeppagari
mesh_terms: D000818:Animals; D001681:Biological Assay / Q000379:methods*; D001688:Biological Products / Q000737:chemistry* / Q000378:metabolism; D002855:Chromatography, Thin Layer / Q000379:methods*; D008049:Lipase / Q000037:antagonists & inhibitors* / Q000378:metabolism; D010179:Pancreas / Q000201:enzymology*; D013045:Species Specificity; D013302:Streptomyces / Q000737:chemistry* / Q000145:classification*; D013552:Swine
chemical_list: D001688:Biological Products; D008049:Lipase
keywords: Autobiographic method; Bromothymol blue; Lipase inhibitor; Orlistat; Streptomyces; p-Nitrophenyl butyrate