title: Effects of low-intensity pulsed ultrasound on healing of mandibular bone defects: an experimental study in rabbits.
abstract: Research evidence suggests that low-intensity pulsed ultrasound (LIPU) produces significant osteoinductive effects, accelerating the healing of bone defects. The authors investigated the effects of LIPU on mandibular bone defects in a rabbit model. Fifty-six adult Dutch rabbits were divided randomly into control, LIPU-1 (1MHz), and LIPU-3 (3MHz) groups. A mandibular defect was created in all rabbits. The effect of LIPU on mandibular defects was assessed by frequency (1 or 3MHz) and timing (2 and 4 weeks). Bone mineral density (BMD) was measured and stereology and histology performed; results were compared at the end of 2 and 4 weeks. LIPU-3 resulted in significantly higher bone formation compared to the control group at the end of week 4 on histological assessment (P=0.008). BMD was significantly higher at 4 weeks than at 2 weeks (P=0.03). LIPU-3 increased the numerical density of osteoblasts and osteocytes at the end of week 4 (P=0.05 and P=0.001, respectively). The results of this study are in favour of using LIPU 3MHz to accelerate mandibular bone healing. However, this study suggests that a frequency of 3MHz and the longer application of LIPU 3MHz for 4 weeks can only promote 8% mandibular bone healing in rabbits. Therefore, the use of LIPU has no really convincing, consistent clinical effects on maxillofacial bone healing.
authors.affiliation: Department of Oral Radiology, School of Dentistry, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: P
authors.initials: P
authors.lastname: Bronoosh
authors.affiliation: Stem Cell and Transgenic Technology Research Centre, Shiraz University of Medical Sciences, Shiraz, Iran; Department of Pharmacology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran. Electronic address: tanidehn@gmail.com.
authors.forename: N
authors.initials: N
authors.lastname: Tanideh
authors.affiliation: Histomorphometry and Stereology Research Centre, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: A
authors.initials: A
authors.lastname: Noorafshan
authors.affiliation: Oral and Dental Health Care Research Centre, Shiraz University of Medical Sciences, Shiraz, Iran; Department of Oral and Maxillofacial Pathology, School of Dentistry, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: A
authors.initials: A
authors.lastname: Andisheh Tadbir
authors.affiliation: Student Research Committee, School of Dentistry, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: M
authors.initials: M
authors.lastname: Aalipanah
authors.affiliation: Department of Oral and Maxillofacial Pathology, School of Dentistry, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: F
authors.initials: F
authors.lastname: Kamali
authors.affiliation: Department of Physiotherapy, School of Rehabilitation, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: K
authors.initials: K
authors.lastname: Abbasnia
authors.affiliation: Laboratory Animals center, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: O
authors.initials: O
authors.lastname: Koohi-Hosseinabadi
mesh_terms: D015502:Absorptiometry, Photon; D000818:Animals; D015519:Bone Density; D004195:Disease Models, Animal; D008338:Mandibular Injuries / Q000628:therapy*; D010006:Osteoblasts / Q000502:physiology; D010011:Osteocytes / Q000502:physiology; D011817:Rabbits; D011897:Random Allocation; D014464:Ultrasonic Therapy / Q000379:methods*; D014945:Wound Healing / Q000502:physiology*
keywords: LIPU; healing; histology; mandible; rabbit; stereology