title: Is Fc gamma receptor IIA (FcγRIIA) polymorphism associated with clinical malaria and Plasmodium falciparum specific antibody levels in children from Burkina Faso?
abstract: In the present study, the influences of FcγRIIA polymorphism on susceptibility to malaria and antibody responses to Plasmodium falciparum antigens were analyzed in children. We recruited 96 healthy children between 3 and 10 years at the beginning of the high transmission season and we followed up for 5 months through the high transmission season to assess the parasitological, immunological and genetic endpoints in relation to clinical malaria status. There was a similar distribution of homozygous and heterozygous individuals carrying the FcγRIIA-131R/R and FcγRIIA-131R/H allele, whereas the number of FcγRIIA-131H/H homozygous individuals was lower. P. falciparum infection frequency was not associated with the FcγRIIa-131R/H polymorphism. Only IgG antibody responses to GLURP R0 showed a significant association between antibody levels and FcγRIIA polymorphism (p=0.02). IgG levels to MSP2a were significantly higher in children who did not experience any clinical malaria episode compared to those who experienced at least one malaria episode (p=0.019). Cytophilic and non-cytophylic IgG subclass levels were higher in children without malaria than those who experienced at least one malaria episode. This difference was statistically significant for IgG1 to MSP3 (p=0.003) and to MSP2a (p=0.006); IgG3 to MSP2a (p=0.007) and to GLURP R0 (p=0.044); IgG2 to MSP2b (p=0.007) and IgG4 to MSP3 (p=0.051) and to MSP2a (p=0.049). In this study, homozygous carriers of the FcγRIIA-131R/R allele had higher malaria-specific antibody levels compare to the heterozygous carriers FcγRIIA-131R/H alleles and to homozygous carriers of FcγRIIA-131H/H alleles. The pre-existing antibodies responses were related to a reduced subsequent risk of clinical malaria.
authors.affiliation: Centre National de Recherche et de Formation sur le Paludisme, Ouagadougou, Burkina Faso; Université de Ouagadougou, Burkina Faso.
authors.forename: Mariama K
authors.initials: MK
authors.lastname: Cherif
authors.affiliation: Centre National de Recherche et de Formation sur le Paludisme, Ouagadougou, Burkina Faso; Polytechnic University of Bobo Dioulasso, Burkina Faso.
authors.forename: Guillaume S
authors.initials: GS
authors.lastname: Sanou
authors.affiliation: Centre National de Recherche et de Formation sur le Paludisme, Ouagadougou, Burkina Faso.
authors.forename: Edith C
authors.initials: EC
authors.lastname: Bougouma
authors.affiliation: Centre National de Recherche et de Formation sur le Paludisme, Ouagadougou, Burkina Faso.
authors.forename: Amidou
authors.initials: A
authors.lastname: Diarra
authors.affiliation: Centre National de Recherche et de Formation sur le Paludisme, Ouagadougou, Burkina Faso.
authors.forename: Alphonse
authors.initials: A
authors.lastname: Ouédraogo
authors.affiliation: Malaria Research and Training Centre, University of Mali, Bamako, Mali.
authors.forename: Amagana
authors.initials: A
authors.lastname: Dolo
authors.affiliation: Department of Immunology, Wenner-Gren Institute, Stockholm University, Sweden.
authors.forename: Marita
authors.initials: M
authors.lastname: Troye-Blomberg
authors.affiliation: Institute of Immunology and Infection Research, University of Edinburgh, EH9 3JT, Scotland, United Kingdom.
authors.forename: David R
authors.initials: DR
authors.lastname: Cavanagh
authors.affiliation: Department of Clinical Biochemistry and Immunology, Statens Serum Institut, Copenhagen, Denmark; Centre for Medical Parasitology at Department of International Health, Immunology and Microbiology, department of Infectious Diseases, Rigshospitalet, University of Copenhagen, Copenhagen, Denmark.
authors.forename: Michael
authors.initials: M
authors.lastname: Theisen
authors.affiliation: Department of Public Health Sciences, University La Sapienza, Rome, Italy.
authors.forename: David
authors.initials: D
authors.lastname: Modiano
authors.affiliation: Centre National de Recherche et de Formation sur le Paludisme, Ouagadougou, Burkina Faso.
authors.forename: Sodiomon B
authors.initials: SB
authors.lastname: Sirima
authors.affiliation: Centre National de Recherche et de Formation sur le Paludisme, Ouagadougou, Burkina Faso. Electronic address: issanebie.cnlp@fasonet.bf.
authors.forename: Issa
authors.initials: I
authors.lastname: Nebié
mesh_terms: D000913:Antibodies, Protozoan / Q000097:blood*; D002050:Burkina Faso / Q000453:epidemiology; D002648:Child; D002675:Child, Preschool; D015331:Cohort Studies; D003430:Cross-Sectional Studies; D004198:Disease Susceptibility; D005260:Female; D006801:Humans; D016778:Malaria, Falciparum / Q000453:epidemiology / Q000235:genetics* / Q000276:immunology; D008297:Male; D010963:Plasmodium falciparum / Q000276:immunology*; D011110:Polymorphism, Genetic; D017452:Receptors, IgG / Q000235:genetics*
chemical_list: D000913:Antibodies, Protozoan; C445323:FCGR2A protein, human; D017452:Receptors, IgG
keywords: Clinical malaria outcome; FcγRIIA polymorphism; IgG and IgG subclass responses