title: Anti-dengue virus envelope protein domain III IgG ELISA among infants with primary dengue virus infections.
abstract: Dengue is the most prevalent arthropod-borne viral illness in humans. The current gold standard serologic test for dengue virus (DENV) infection is a neutralizing antibody assay. We examined a DENV recombinant (r)E protein domain III IgG ELISA among infants with primary DENV infections. Infants experience a primary DENV infection in the presence of maternally derived anti-DENV IgG. The estimated DENV rE protein domain III IgG levels to the infecting serotype at the time of infant primary symptomatic DENV2 and DENV3 infections correlated with the 50% plaque reduction neutralization reciprocal antibody titers (PRNT50). Anti-DENVs 1-4 rE protein domain III IgG levels all correlated with each other, and the estimated rE protein domain III IgG level to the infecting serotype at the time of infection inversely correlated with dengue disease severity. The anti-DENV rE protein domain III IgG ELISA may be a useful and potentially high-throughput alternative to traditional DENV neutralizing antibody assays.
authors.affiliation: Division of Infectious Diseases and Immunology, Department of Medicine, University of Massachusetts Medical School, Worcester, MA, USA. Electronic address: daniel.libraty@umassmed.edu.
authors.forename: Daniel H
authors.initials: DH
authors.lastname: Libraty
authors.affiliation: Division of Infectious Diseases and Immunology, Department of Medicine, University of Massachusetts Medical School, Worcester, MA, USA.
authors.forename: Lei
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department of Medicine, Research Institute for Tropical Medicine, Manila, Philippines.
authors.forename: AnaMae
authors.initials: A
authors.lastname: Obcena
authors.affiliation: San Pablo City Health Office, San Pablo, Philippines.
authors.forename: Job D
authors.initials: JD
authors.lastname: Brion
authors.affiliation: Department of Medicine, Research Institute for Tropical Medicine, Manila, Philippines; Department of Microbiology, Research Institute for Tropical Medicine, Manila, Philippines.
authors.forename: Rosario Z
authors.initials: RZ
authors.lastname: Capeding
mesh_terms: D000328:Adult; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology*; D057134:Antibodies, Neutralizing / Q000276:immunology*; D000914:Antibodies, Viral / Q000276:immunology*; D003715:Dengue / Q000821:virology*; D003716:Dengue Virus / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology*; D007223:Infant; D008297:Male; D011237:Predictive Value of Tests; D017434:Protein Structure, Tertiary; D012698:Serologic Tests; D012720:Severity of Illness Index; D014759:Viral Envelope Proteins
chemical_list: D000888:Antibodies, Anti-Idiotypic; D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D007074:Immunoglobulin G; D014759:Viral Envelope Proteins; C048844:anti-IgG
keywords: Dengue; ELISA; Envelope protein; Serologic assay