title: Tracheal regeneration using polycaprolactone/collagen-nanofiber coated with umbilical cord serum after partial resection.
abstract: OBJECTIVE
  We developed a PCL/collagen nanofiber (PC-NF) scaffold/human umbilical cord serum (hUCS) to facilitate epithelial cell migration after implantation in order to promote regeneration of the tracheal epithelium without having a cell source.
  
  
  METHODS
  The isolated chodrocytes from bovine auricle were used to evaluate the cellular activities cultured in the scaffolds with or without hUCS. A 4mm wide/8mm long, full thickness anterior defect was created in the tracheal rings of rats. An anterior tracheal defect was implanted with a PCL/collagen-NF scaffold (PC-NF) in the control group (n=7), and a PCL/collagen-NF coated with hUCS scaffold (PCU-NF) was implanted in the experimental group (n=7). All rats were sacrificed at 7 weeks postoperatively. The cervical trachea, including the implant site, was resected, and gross and histological examinations were performed.
  
  
  RESULTS
  The viable cells of PCU-NF scaffold were significantly higher than that of the PCL and PC-NF scaffold at 7 days; the result can be due to the exceptional biological growth factors of the hUCS. The steromicroscopic finding showed that the artificial trachea was covered by perichondrium without dislocation or granulation at 7 weeks postsurgery. When compared to the control group, the PCU-NF group showed a completely regenerated tracheal wall with luminal epilthelization.
  
  
  CONCLUSIONS
  As a results of this study, the PCU-NF scaffold promoted cartilage and epithelial regeneration over the artificial trachea without graft inflammation. Partial tracheal reconstruction using PCU-NF scaffold is suitable for enhancing cartilage and epithelial regeneration.
authors.affiliation: Ecobio Laboratory, Gwangju, South Korea.
authors.forename: Yoon Seok
authors.initials: YS
authors.lastname: Jang
authors.affiliation: Department of Otolaryngology, Chonnam National University Medical School, Gwangju, South Korea. Electronic address: chulsavio@hanmail.net.
authors.forename: Chul Ho
authors.initials: CH
authors.lastname: Jang
authors.affiliation: Department of Otolaryngology, Chonnam National University Medical School, Gwangju, South Korea.
authors.forename: Yong Beom
authors.initials: YB
authors.lastname: Cho
authors.affiliation: Department of Bio-Mechatronics, Sungkyunkwan University (SKKU), Suwon, South Korea.
authors.forename: Minseong
authors.initials: M
authors.lastname: Kim
authors.affiliation: Department of Bio-Mechatronics, Sungkyunkwan University (SKKU), Suwon, South Korea. Electronic address: gkimbme@skku.edu.
authors.forename: Geun Hyung
authors.initials: GH
authors.lastname: Kim
mesh_terms: D000818:Animals; D002417:Cattle; D020099:Coated Materials, Biocompatible; D024042:Collagen Type I; D005312:Fetal Blood; D057139:Nanofibers; D011091:Polyesters; D012038:Regeneration; D023822:Tissue Engineering; D054457:Tissue Scaffolds; D014132:Trachea / Q000502:physiology* / Q000601:surgery
chemical_list: D020099:Coated Materials, Biocompatible; D024042:Collagen Type I; D011091:Polyesters; C016240:polycaprolactone
keywords: Collagen; Nanofiber; PCL; Regeneration; Trachea; Umbilical cord serum