title: Construction of transformation system in Penicillium purpurogenum.
abstract: Penicillium purpurogenum attracts attention in the food industry and biomass degradation. We expressed green fluorescent protein (GFP) with pBPE, a novel vector, and constructed a transformation system for P. purpurogenum. The accumulation of GFP was confirmed by fluorescence microscopy. In future, this system may prove useful for the genetic modification of P. purpurogenum.
authors.affiliation: Department of Chemistry and Life Science, Nihon University College of Bioresource Sciences, 1866 Kameino, Fujisawa, Kanagawa 252-0880, Japan.
authors.forename: Ryo
authors.initials: R
authors.lastname: Kojima
authors.affiliation: Department of Chemistry and Life Science, Nihon University College of Bioresource Sciences, 1866 Kameino, Fujisawa, Kanagawa 252-0880, Japan.
authors.forename: Teppei
authors.initials: T
authors.lastname: Arai
authors.affiliation: Department of Chemistry and Life Science, Nihon University College of Bioresource Sciences, 1866 Kameino, Fujisawa, Kanagawa 252-0880, Japan.
authors.forename: Takafumi
authors.initials: T
authors.lastname: Kasumi
authors.affiliation: Department of Chemistry and Life Science, Nihon University College of Bioresource Sciences, 1866 Kameino, Fujisawa, Kanagawa 252-0880, Japan. Electronic address: j-ogihara@brs.nihon-u.ac.jp.
authors.forename: Jun
authors.initials: J
authors.lastname: Ogihara
mesh_terms: D005822:Genetic Vectors / Q000235:genetics; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D008856:Microscopy, Fluorescence; D010407:Penicillium / Q000235:genetics* / Q000378:metabolism; D011401:Promoter Regions, Genetic / Q000235:genetics; D011523:Protoplasts / Q000378:metabolism; D014169:Transformation, Bacterial
chemical_list: D049452:Green Fluorescent Proteins
keywords: Green fluorescent protein; Penicillium purpurogenum; Promoter; Protoplasts; Terminator; Transformation