title: Identification and characterisation of the anti-oxidative stress properties of the lamprey prohibitin 2 gene.
abstract: The highly conserved protein prohibitin 2 (PHB2) has been implicated as a cell-surface receptor in the regulation of proliferation, apoptosis, transcription, and mitochondrial protein folding. In the present study, we identified a Lampetra morii homologue of PHB2, Lm-PHB2, showing greater than 61.8% sequence identity with its homologues. Phylogenetic analysis indicated that the position of Lm-PHB2 is consistent with lamprey phylogeny. Expression of the Lm-PHB2 protein was nearly equivalent in the heart, liver, kidneys, intestines, and muscles of normal lampreys. However, the Lm-PHB2 protein was down-regulated in the myocardia of lampreys challenged for 5 days with adriamycin (Adr), followed by a significant up-regulation 10 days after treatment. In vitro, recombinant Lm-PHB2 (rLm-PHB2) protein could significantly enhance the H2O2-induced oxidative stress tolerance in Chang liver (CHL) cells. Further mechanism studies indicated that the nucleus-to-mitochondria translocation of Lm-PHB2 was closely involved in the oxidative stress protection. Our results suggests that the strategies to modulate Lm-PHB2 levels may constitute a novel therapeutic approach for myocardial injury and liver inflammatory diseases, conditions in which oxidative stress plays a critical role in tissue injury and inflammation.
authors.affiliation: Life Science College of Liaoning Normal University, Dalian 116029, China; Lamprey Research Centre, Liaoning Normal University, Dalian 116029, China. Electronic address: sally_ts_li@163.com.
authors.forename: Tiesong
authors.initials: T
authors.lastname: Li
authors.affiliation: Life Science College of Liaoning Normal University, Dalian 116029, China; Lamprey Research Centre, Liaoning Normal University, Dalian 116029, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Life Science College of Liaoning Normal University, Dalian 116029, China; Lamprey Research Centre, Liaoning Normal University, Dalian 116029, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Gao
authors.affiliation: Life Science College of Liaoning Normal University, Dalian 116029, China; Lamprey Research Centre, Liaoning Normal University, Dalian 116029, China. Electronic address: liqw@263.net.
authors.forename: Qingwei
authors.initials: Q
authors.lastname: Li
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics / Q000378:metabolism; D005260:Female; D029941:Fish Proteins / Q000235:genetics* / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects*; D006861:Hydrogen Peroxide / Q000494:pharmacology; D007798:Lampreys / Q000235:genetics* / Q000276:immunology* / Q000378:metabolism; D008297:Male; D008969:Molecular Sequence Data; D009928:Organ Specificity; D018384:Oxidative Stress / Q000187:drug effects*; D010802:Phylogeny; D000091402:Prohibitins; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction / Q000662:veterinary; D012097:Repressor Proteins / Q000235:genetics* / Q000378:metabolism; D016415:Sequence Alignment / Q000662:veterinary
chemical_list: D018076:DNA, Complementary; D029941:Fish Proteins; C000717331:PHB2 protein, human; D000091402:Prohibitins; D012333:RNA, Messenger; D012097:Repressor Proteins; D006861:Hydrogen Peroxide
keywords: Human chang liver cells; Lamprey; Oxidative stress; Prohibitin; Translocation