title: Early prediction of severe acute pancreatitis by urinary β-2 microglobulin/saposin B peak ratios on MALDI-TOF.
abstract: The current methods for predicting severe acute pancreatitis (severe AP) are either complicated or lack efficient sensitivity and specificity. In this study, a simple and practical approach was developed to predict severe AP by using peak intensity ratio of urinary β-2 microglobulin (B2M) to saposin B (SB) on MALDI-TOF MS. Patients with B2M/SB ratio higher than 1.127 present severe AP symptom with a higher Ranson score, computed tomography (CT) grade and longer hospitalization with a sensitivity of 83.7% and specificity of 74.3%. Label-free quantitative proteomics by nanoLC-MS/MS was applied to urine of severe AP patients and found that severe AP is accompanied with kidney injury and inflammation. The measurement of B2M/SB ratios by MALDI-TOF MS could be a simple, accurate and rapid method to diagnose severe AP as well as to monitor AP progression.
authors.affiliation: College of Medicine, China Medical University, Taichung, Taiwan; Division of Nephrology, China Medical University Hospital, Taichung, Taiwan.
authors.forename: Chiz-Tzung
authors.initials: CT
authors.lastname: Chang
authors.affiliation: Proteomics Core Laboratory, Department of Medical Research, China Medical University Hospital, Taichung 40402, Taiwan.
authors.forename: Hsin-Yi
authors.initials: HY
authors.lastname: Liao
authors.affiliation: College of Medicine, China Medical University, Taichung, Taiwan; Division of Gastroenterology, China Medical University Hospital, Taichung, Taiwan.
authors.forename: Wen-Hsin
authors.initials: WH
authors.lastname: Huang
authors.affiliation: College of Medicine, China Medical University, Taichung, Taiwan; Division of Nephrology, China Medical University Hospital, Taichung, Taiwan.
authors.forename: Shih-Yi
authors.initials: SY
authors.lastname: Lin
authors.affiliation: Division of Gastroenterology, China Medical University Hospital, Taichung, Taiwan.
authors.forename: Tsung-Yu
authors.initials: TY
authors.lastname: Tsai
authors.affiliation: L5 Research Center, China Medical University Hospital, Taichung, Taiwan; Section of Atherosclerosis and Lipoprotein Research, Department of Medicine, Baylor College of Medicine, Houston, TX 77030, United States.
authors.forename: Chao-Yuh
authors.initials: CY
authors.lastname: Yang
authors.affiliation: Department of Medical Genetics, Pediatrics and Medical Research, China Medical University Hospital, Taichung 40402, Taiwan.
authors.forename: Fuu-Jen
authors.initials: FJ
authors.lastname: Tsai
authors.affiliation: Proteomics Core Laboratory, Department of Medical Research, China Medical University Hospital, Taichung 40402, Taiwan; Graduate Institute of Integrated Medicine, China Medical University, Taichung 40402, Taiwan. Electronic address: cjchen@mail.cmu.edu.tw.
authors.forename: Chao-Jung
authors.initials: CJ
authors.lastname: Chen
mesh_terms: D000208:Acute Disease; D000328:Adult; D000368:Aged; D000595:Amino Acid Sequence; D016022:Case-Control Studies; D041881:Cholecystitis, Acute / Q000652:urine; D002853:Chromatography, Liquid / Q000379:methods; D005260:Female; D006801:Humans; D007902:Length of Stay; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D009393:Nephritis / Q000652:urine; D010195:Pancreatitis / Q000175:diagnosis* / Q000652:urine*; D011237:Predictive Value of Tests; D049231:Saposins / Q000652:urine*; D012680:Sensitivity and Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D053719:Tandem Mass Spectrometry; D014057:Tomography, X-Ray Computed; D001613:beta 2-Microglobulin / Q000652:urine*
chemical_list: D049231:Saposins; D001613:beta 2-Microglobulin
keywords: Biomarkers; Label-free; Mass spectrometry; Quantitative proteomics; Severe acute pancreatitis