title: Development and characterization of simple sequence repeat (SSR) markers based on a full-length cDNA library of Scutellaria baicalensis.
abstract: Scutellaria baicalensis Georgi is an herbaceous perennial plant used as one of the staple Chinese herbal medicines in China with a long officinal history. However, research on S. baicalensis is currently limited due to the lack of genome and gene expression information. A full-length cDNA library from leaves and roots of S. baicalensis subjected to water deficit and heat, conditions that have been shown to affect baicalein accumulation, was constructed. There were 6491 expressed sequence tags (ESTs) obtained. UniGenes were assembled by BLAST similarity searches and annotated with Gene Ontology (GO) and the Kyoto Encyclopedia of Genes and Genomes (KEGG). A total of 78 simple sequence repeats (SSRs) were identified and SSR markers associated with the active ingredients of S. baicalensis were selected. EST-SSR transferability was determined from 5 populations from different areas. This study is the first to produce a large volume of gene expression data from S. baicalensis to facilitate gene discovery in S. baicalensis and provide an important resource for molecular genetic and functional genomic studies in this species.
authors.affiliation: State Key Laboratory Breeding Base of Dao-di Herbs, National Resource Center for Chinese Materia Medica, Academy of Chinese Medical Sciences, Beijing 100700, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Yuan
authors.affiliation: State Key Laboratory Breeding Base of Dao-di Herbs, National Resource Center for Chinese Materia Medica, Academy of Chinese Medical Sciences, Beijing 100700, China; Baotou Medical College, No. 31 Construction Road, Baotou, Inner Mongolia 014060, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Long
authors.affiliation: State Key Laboratory Breeding Base of Dao-di Herbs, National Resource Center for Chinese Materia Medica, Academy of Chinese Medical Sciences, Beijing 100700, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Jiang
authors.affiliation: Baotou Medical College, No. 31 Construction Road, Baotou, Inner Mongolia 014060, China.
authors.forename: Minhui
authors.initials: M
authors.lastname: Li
authors.affiliation: State Key Laboratory Breeding Base of Dao-di Herbs, National Resource Center for Chinese Materia Medica, Academy of Chinese Medical Sciences, Beijing 100700, China. Electronic address: huangluqi01@126.com.
authors.forename: Luqi
authors.initials: L
authors.lastname: Huang
mesh_terms: D020224:Expressed Sequence Tags; D018506:Gene Expression Regulation, Plant; D015723:Gene Library; D018895:Microsatellite Repeats; D018515:Plant Leaves / Q000235:genetics; D010940:Plant Proteins / Q000235:genetics*; D018517:Plant Roots / Q000235:genetics; D018749:RNA, Plant; D031364:Scutellaria baicalensis / Q000033:anatomy & histology / Q000145:classification / Q000235:genetics*; D017422:Sequence Analysis, DNA / Q000379:methods; D013312:Stress, Physiological
chemical_list: D010940:Plant Proteins; D018749:RNA, Plant
keywords: Active compounds; EST-SSR; Full-length cDNA; Scutellaria baicalensis