title: Evaluation of repetitive element polymerase chain reaction for surveillance of methicillin-resistant Staphylococcus aureus at a large academic medical center and community hospitals.
abstract: Repetitive element polymerase chain reaction (rep-PCR) typing has been used for methicillin-resistant Staphylococcus aureus (MRSA) strain characterization. The goal of this study was to determine if a rapid commercial rep-PCR system, DiversiLab™ (DL; bioMérieux, Durham, NC, USA), could be used for MRSA surveillance at a large medical center and community hospitals. A total of 1286 MRSA isolates genotyped by the DL system were distributed into 84 distinct rep-PCR patterns: 737/1286 (57%) were clustered into 6 major rep-PCR patterns. A subset of 220 isolates was further typed by pulsed-field gel electrophoresis (PFGE), spa typing, and SCCmec typing. The 220 isolates were distributed into 80 rep-PCR patterns, 94 PFGE pulsotypes, 27 spa, and 3 SCCmec types. The DL rep-PCR system is sufficient for surveillance, but the DL system alone cannot be used to compare data to other institutions until a standardized nomenclature is established and the DL MRSA reference library is expanded.
authors.affiliation: Department of Internal Medicine, Division of Infectious Diseases, The Ohio State University Wexner Medical Center, N-1147 Doan Hall, 410 West 10th Ave, Columbus, OH 43210. Electronic address: Shu-Hua.Wang@osumc.edu.
authors.forename: Shu-Hua
authors.initials: SH
authors.lastname: Wang
authors.affiliation: Department of Internal Medicine, Division of Infectious Diseases, The Ohio State University Wexner Medical Center, N-1147 Doan Hall, 410 West 10th Ave, Columbus, OH 43210.
authors.forename: Kurt B
authors.initials: KB
authors.lastname: Stevenson
authors.affiliation: Department of Internal Medicine, Division of Infectious Diseases, The Ohio State University Wexner Medical Center, N-1147 Doan Hall, 410 West 10th Ave, Columbus, OH 43210.
authors.forename: Lisa
authors.initials: L
authors.lastname: Hines
authors.affiliation: Public Health Research Institute, Rutgers University, 225 Warren Street, Room W210N, Newark, NJ 07103.
authors.forename: José R
authors.initials: JR
authors.lastname: Mediavilla
authors.affiliation: Department of Internal Medicine, Division of Infectious Diseases, The Ohio State University Wexner Medical Center, N-1147 Doan Hall, 410 West 10th Ave, Columbus, OH 43210.
authors.forename: Yosef
authors.initials: Y
authors.lastname: Khan
authors.affiliation: Clinical Microbiology, Department of Pathology, The Ohio State University Wexner Medical Center, 1492 E Broad Street, Columbus, OH 43205.
authors.forename: Ruchi
authors.initials: R
authors.lastname: Soni
authors.affiliation: bioMérieux, Inc, 100 Rodolphe Street, Durham, NC 27712.
authors.forename: Wendy
authors.initials: W
authors.lastname: Dutch
authors.affiliation: The Ohio Department of Health Laboratory, 8995 East Main Street, Bldg 22 Reynoldsburg, OH 43068.
authors.forename: Eric
authors.initials: E
authors.lastname: Brandt
authors.affiliation: The Ohio Department of Health Laboratory, 8995 East Main Street, Bldg 22 Reynoldsburg, OH 43068.
authors.forename: Tammy
authors.initials: T
authors.lastname: Bannerman
authors.affiliation: Public Health Research Institute, Rutgers University, 225 Warren Street, Room W210N, Newark, NJ 07103.
authors.forename: Barry N
authors.initials: BN
authors.lastname: Kreiswirth
authors.affiliation: Clinical Microbiology, Department of Pathology, The Ohio State University Wexner Medical Center, 1492 E Broad Street, Columbus, OH 43205. Electronic address: Preeti.Pancholi@osumc.edu.
authors.forename: Preeti
authors.initials: P
authors.lastname: Pancholi
mesh_terms: D000046:Academic Medical Centers; D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015373:Bacterial Typing Techniques / Q000379:methods; D016521:Electrophoresis, Gel, Pulsed-Field; D005260:Female; D006764:Hospitals, Community; D006801:Humans; D017053:Infection Control / Q000379:methods*; D008297:Male; D055624:Methicillin-Resistant Staphylococcus aureus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D008875:Middle Aged; D017720:Molecular Epidemiology / Q000379:methods; D009820:Ohio; D016133:Polymerase Chain Reaction / Q000379:methods*; D012091:Repetitive Sequences, Nucleic Acid; D013203:Staphylococcal Infections / Q000382:microbiology*; D055815:Young Adult
keywords: MRSA; Methicillin-resistant Staphylococcus aureus; PFGE; SCCmec; molecular genotyping; rep-PCR; spa