title: Linear epitope recognition antibodies strongly respond to the C-terminal domain of HP-PRRSV GP5.
abstract: A total of 155 peptides derived from the highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV) glycoprotein 5 (GP5) were printed on a chip to reveal the antigen reaction characteristics of the protein. The reactions of these peptides to HP-PRRSV-specific pig serum were scanned and quantified using fluorescence intensity via the PepSlide(®) Analyzer software. The intensity plots showed different reactions in the different sectors of GP5. The highest reaction intensity value reached 3894.5, with a peptide sequence of IVEKGGKVEVEGHLI. Seventeen peptides that showed relatively high reaction levels with HP-PRRSV-specific pig serum were selected as epitope candidates. Furthermore, the antigenic character was predicted using a software and was compared with the peptide scan results. In contrast to the software prediction, the HP-PRRSV-specific antibodies strongly responded to the C-terminal domain of GP5. The acquired data may be useful for understanding the antigenic characteristics of HP-PRRSV GP5.
authors.affiliation: College of Veterinary Medicine, Northwest A&F University, No. 22 Xinong road, Yangling 712100, China. Electronic address: wxlong@nwsuaf.edu.cn.
authors.forename: Xinglong
authors.initials: X
authors.lastname: Wang
authors.affiliation: College of Veterinary Medicine, Northwest A&F University, No. 22 Xinong road, Yangling 712100, China.
authors.forename: Li
authors.initials: L
authors.lastname: Qui
authors.affiliation: Shaanxi University of Technology, Hanzhong 723001, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Dang
authors.affiliation: College of Veterinary Medicine, Northwest A&F University, No. 22 Xinong road, Yangling 712100, China.
authors.forename: Sha
authors.initials: S
authors.lastname: Xiao
authors.affiliation: College of Veterinary Medicine, Northwest A&F University, No. 22 Xinong road, Yangling 712100, China.
authors.forename: Shuxia
authors.initials: S
authors.lastname: Zhang
authors.affiliation: College of Veterinary Medicine, Northwest A&F University, No. 22 Xinong road, Yangling 712100, China. Electronic address: yzq8162@163.com.
authors.forename: Zengqi
authors.initials: Z
authors.lastname: Yang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology*; D000939:Epitopes / Q000276:immunology; D008969:Molecular Sequence Data; D010455:Peptides / Q000276:immunology; D019318:Porcine Reproductive and Respiratory Syndrome / Q000276:immunology*; D019316:Porcine respiratory and reproductive syndrome virus / Q000276:immunology*; D013552:Swine; D014759:Viral Envelope Proteins / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D000939:Epitopes; D010455:Peptides; D014759:Viral Envelope Proteins; C114789:glycoprotein 5, PRRSV
keywords: Antigenic regions; HP-PRRSV; Peptide scan