title: In vitro anticancer activity of fucoidan from Turbinaria conoides against A549 cell lines.
abstract: The present study was conducted to evaluate the anticancer activity of fucoidan isolated from brown seaweed Turbinaria conoides. Extracted fucoidan contained 53 ± 0.69% of fucose and 38 ± 0.42% of sulphate, respectively. Functional groups and structural characteristics of the fucoidan were analyzed by FT-IR and NMR. In vitro anticancer effect was studied on A549 cell line. Fucoidan inhibited the growth of cancer cells in a dose-dependent manner and potent anticancer activities were 24.9-73.5% in the concentrations of 31.25-500 μg/ml. The CTC50 value against the cancer cell was found to be 45 μg/ml and the CTC50 value of normal Vero cell line is 325 μg/ml. This study suggests that the fucoidan from T. conoides could be significantly improved if the active component is further purified and tested for further investigation in various cancer cell lines.
authors.affiliation: Centre of Advanced Study in Marine Biology, Faculty of Marine Sciences Annamalai University, Parangipettai 608502, Tamil Nadu, India; Centre for Ocean Research, Sathyabama University, Jeppiaar Nagar, Chennai 600119, India. Electronic address: tmarudhu@gmail.com.
authors.forename: Thangapandi
authors.initials: T
authors.lastname: Marudhupandi
authors.affiliation: Centre of Advanced Study in Marine Biology, Faculty of Marine Sciences Annamalai University, Parangipettai 608502, Tamil Nadu, India; National Bureau of Fish Genetic Resources, Indian Council of Agriculture Research, Lucknow, Uttar Pradesh 226002, India.
authors.forename: Thipramalai Thankappan
authors.initials: TT
authors.lastname: Ajith Kumar
authors.affiliation: CMS College of Science and Commerce, Chinnavedampati (PO), Coimbatore, Tamil Nadu, India.
authors.forename: Shanmugaasokan
authors.initials: S
authors.lastname: Lakshmanasenthil
authors.affiliation: CMS College of Science and Commerce, Chinnavedampati (PO), Coimbatore, Tamil Nadu, India.
authors.forename: Gunasekaran
authors.initials: G
authors.lastname: Suja
authors.affiliation: CMS College of Science and Commerce, Chinnavedampati (PO), Coimbatore, Tamil Nadu, India.
authors.forename: Thirumalairaj
authors.initials: T
authors.lastname: Vinothkumar
mesh_terms: D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry* / Q000494:pharmacology*; D045744:Cell Line, Tumor; D002522:Chlorocebus aethiops; D004305:Dose-Response Relationship, Drug; D006801:Humans; D019906:Nuclear Magnetic Resonance, Biomolecular; D011134:Polysaccharides / Q000737:chemistry* / Q000494:pharmacology*; D012624:Seaweed / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D014709:Vero Cells
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D011134:Polysaccharides; C007789:fucoidan
keywords: A549 cell line; Anticancer; Fucoidan; NMR