title: MiR-20a regulates the PRKG1 gene by targeting its coding region in pulmonary arterial smooth muscle cells.
abstract: Chronic hypoxia triggers pulmonary vascular remodeling, which is associated with de-differentiation of pulmonary artery smooth muscle cells (PASMC). Here, we show that miR-20a expression is up-regulated in response to hypoxia in both mouse and human PASMC. We also observed that miR-20a represses the protein kinase, cGMP-dependent, type I (PRKG1) gene and we identified two crucial miR-20a binding sites within the coding region of PRKG1. Functional studies showed that miR-20a promotes the proliferation and migration of human PASMC, whereas it inhibits their differentiation. In summary, we provided a possible mechanism by which hypoxia results in decreased PRKG1 expression and in the phenotypic switching of PASMC.
authors.affiliation: Shenzhen Key Laboratory of Microbial Genetic Engineering, College of Life Sciences, Shenzhen University, Shenzhen, Guangdong 518060, China; Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, College of Optoelectronic Engineering, Shenzhen University, Shenzhen, Guangdong, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zeng
authors.affiliation: Shenzhen Key Laboratory of Microbial Genetic Engineering, College of Life Sciences, Shenzhen University, Shenzhen, Guangdong 518060, China.
authors.forename: Yanping
authors.initials: Y
authors.lastname: Pan
authors.affiliation: Shenzhen Key Laboratory of Microbial Genetic Engineering, College of Life Sciences, Shenzhen University, Shenzhen, Guangdong 518060, China.
authors.forename: Hongtai
authors.initials: H
authors.lastname: Liu
authors.affiliation: Shenzhen Key Laboratory of Microbial Genetic Engineering, College of Life Sciences, Shenzhen University, Shenzhen, Guangdong 518060, China.
authors.forename: Kang
authors.initials: K
authors.lastname: Kang
authors.affiliation: Shenzhen Key Laboratory of Microbial Genetic Engineering, College of Life Sciences, Shenzhen University, Shenzhen, Guangdong 518060, China.
authors.forename: Yike
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Department of Chest Surgery, Peking University Shenzhen Hospital, Shenzhen, Guangdong 518000, China.
authors.forename: Gang
authors.initials: G
authors.lastname: Hui
authors.affiliation: Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, College of Optoelectronic Engineering, Shenzhen University, Shenzhen, Guangdong, China.
authors.forename: Wenda
authors.initials: W
authors.lastname: Peng
authors.affiliation: Department of Pediatrics, University of Illinois at Chicago, Chicago, IL 60612, USA.
authors.forename: Ramaswamy
authors.initials: R
authors.lastname: Ramchandran
authors.affiliation: Department of Pediatrics, University of Illinois at Chicago, Chicago, IL 60612, USA.
authors.forename: J Usha
authors.initials: JU
authors.lastname: Raj
authors.affiliation: Shenzhen Key Laboratory of Microbial Genetic Engineering, College of Life Sciences, Shenzhen University, Shenzhen, Guangdong 518060, China; Department of Pediatrics, University of Illinois at Chicago, Chicago, IL 60612, USA. Electronic address: dmgou@szu.edu.cn.
authors.forename: Deming
authors.initials: D
authors.lastname: Gou
mesh_terms: D000818:Animals; D001483:Base Sequence; D002454:Cell Differentiation / Q000235:genetics; D015687:Cell Hypoxia; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics; D017124:Conserved Sequence; D062965:Cyclic GMP-Dependent Protein Kinase Type I / Q000235:genetics*; D015971:Gene Expression Regulation, Enzymologic / Q000235:genetics; D006801:Humans; D008168:Lung / Q000378:metabolism; D051379:Mice; D035683:MicroRNAs / Q000235:genetics*; D032389:Myocytes, Smooth Muscle / Q000166:cytology / Q000378:metabolism*; D016366:Open Reading Frames / Q000235:genetics*; D010641:Phenotype; D011651:Pulmonary Artery / Q000166:cytology*
chemical_list: C534451:MIRN20a microRNA, human; D035683:MicroRNAs; D062965:Cyclic GMP-Dependent Protein Kinase Type I; C569718:PRKG1 protein, human; C569720:Prkg1 protein, mouse
keywords: Coding region; Hypoxia; MicroRNA; Pulmonary arterial smooth muscle cells; cGMP-dependent protein kinase