title: Isolation and characterization of microalgae for biodiesel production from seawater.
abstract: As green marine microalgae isolated from local seawater in Tianjin, China, Nannochloropsis gaditana Q6 was tolerant to the variation of salinity with the highest biomass and lipid concentration in natural seawater medium. Although this strain could grow mixotrophically with glycerol, the narrow gap between mixotrophic and autotrophic cultivation suggested that autotrophic cultivation was the optimal trophic type for N. gaditana Q6 growth. In addition, strain Q6 was more sensitive to the variance of NH4HCO3 concentration than NaH2PO4 concentration. Consequently, the lipid production could be maximized by the two-stage cultivation strategy, with an initial high NH4HCO3 concentration for biomass production followed by low NH4HCO3 concentration for lipid accumulation.
authors.affiliation: School of Environment Science and Engineering/State Key Lab of Engines, Tianjin University, Tianjin 300072, China; Tianjin Technological Engineering Center on Biomass-derived Gas and Oil, No. 92, Weijin Rd., Nankai District, Tianjin 300072, China.
authors.forename: Liu
authors.initials: L
authors.lastname: Zhao
authors.affiliation: School of Environment Science and Engineering/State Key Lab of Engines, Tianjin University, Tianjin 300072, China; Tianjin Technological Engineering Center on Biomass-derived Gas and Oil, No. 92, Weijin Rd., Nankai District, Tianjin 300072, China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Qi
authors.affiliation: School of Environment Science and Engineering/State Key Lab of Engines, Tianjin University, Tianjin 300072, China; Tianjin Technological Engineering Center on Biomass-derived Gas and Oil, No. 92, Weijin Rd., Nankai District, Tianjin 300072, China. Electronic address: chen@tju.edu.cn.
authors.forename: Guanyi
authors.initials: G
authors.lastname: Chen
mesh_terms: D052818:Autotrophic Processes / Q000187:drug effects; D056804:Biofuels / Q000382:microbiology*; D018533:Biomass; D002244:Carbon / Q000494:pharmacology; D008055:Lipids / Q000096:biosynthesis; D058086:Microalgae / Q000187:drug effects / Q000302:isolation & purification*; D009584:Nitrogen / Q000494:pharmacology; D010758:Phosphorus / Q000494:pharmacology; D054712:Salinity; D012623:Seawater / Q000382:microbiology*
chemical_list: D056804:Biofuels; D008055:Lipids; D010758:Phosphorus; D002244:Carbon; D009584:Nitrogen
keywords: Carbon; Nannochloropsis gaditana; Nitrogen; Phosphorus; Salinity