title: Short N-terminal region of UDP-galactose transporter (SLC35A2) is crucial for galactosylation of N-glycans.
abstract: UDP-galactose transporter (UGT) and UDP-N-acetylglucosamine transporter (NGT) form heterologous complexes in the Golgi apparatus (GA) membrane. We aimed to identify UGT region responsible for galactosylation of N-glycans. Chimeric proteins composed of human UGT and either NGT or CMP-sialic acid transporter (CST) localized to the GA, and all but UGT/CST chimera corrected galactosylation defect in UGT-deficient cell lines, although at different efficiency. Importantly, short N-terminal region composed of 35 N-terminal amino-acid residues of UGT was crucial for galactosylation of N-glycans. The remaining molecule must be derived from NGT not CST, confirming that the role played by UGT and NGT is coupled.
authors.affiliation: Laboratory of Biochemistry, Faculty of Biotechnology, University of Wroclaw, 14A F. Joliot-Curie St., 50-383 Wroclaw, Poland.
authors.forename: Paulina
authors.initials: P
authors.lastname: Sosicka
authors.affiliation: Laboratory of Biotechnology, Faculty of Biotechnology, University of Wroclaw, 14A F. Joliot-Curie St., 50-383 Wroclaw, Poland.
authors.forename: Piotr
authors.initials: P
authors.lastname: Jakimowicz
authors.affiliation: Laboratory of Biochemistry, Faculty of Biotechnology, University of Wroclaw, 14A F. Joliot-Curie St., 50-383 Wroclaw, Poland.
authors.forename: Teresa
authors.initials: T
authors.lastname: Olczak
authors.affiliation: Laboratory of Biochemistry, Faculty of Biotechnology, University of Wroclaw, 14A F. Joliot-Curie St., 50-383 Wroclaw, Poland. Electronic address: Mariusz.Olczak@biotech.uni.wroc.pl.
authors.forename: Mariusz
authors.initials: M
authors.lastname: Olczak
mesh_terms: D000818:Animals; D016466:CHO Cells; D003412:Cricetulus; D004285:Dogs; D005690:Galactose / Q000378:metabolism*; D006031:Glycosylation; D006801:Humans; D061985:Madin Darby Canine Kidney Cells; D009004:Monosaccharide Transport Proteins / Q000378:metabolism*; D011134:Polysaccharides / Q000737:chemistry* / Q000378:metabolism*; D000099237:UDP-Galactose Translocators
chemical_list: D005690:Galactose; D009004:Monosaccharide Transport Proteins; D011134:Polysaccharides; D000099237:UDP-Galactose Translocators
keywords: Chimeric protein; Glycosylation; Golgi apparatus; UDP-N-acetylglucosamine transporter; UDP-galactose transporter