title: Spectroscopic studies on the in vitro antioxidant capacity of isopentyl ferulate.
abstract: Essential oils have played a prominent role in research on natural products, due to the high level of bioactive constituents, which include those derived from phenylpropanoids or terpenoids. This study aimed to evaluate the antioxidant capacity of isopentyl ferulate (IF) employing in vitro experimental models for elimination of the 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2'-azinobis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS+), hydroxyl (OH) and nitric oxide (NO), as well as its capacity to electron transfer by reducing potential and inhibition of lipid peroxidation by TBARS (thiobarbituric acid reactive substances) method. In all in vitro antioxidants protocols, isopentyl ferulate showed to be potent in a concentration of 54.4 nM, presenting a percentage inhibition of 91.29±0.57, 92.63±0.28, 83.62±0.18, 77.07±0.72 and 79.51±0.32% for DPPH, ABTS+, hydroxyl, nitric oxide and TBARS level, respectively. The increase of absorbance at 700 nm in the concentrations of 3.4, 6.8, 13.6, 27.2 and 54.4 nM shows the reducing potential of IF. Similar results were obtained with Trolox (559 nM), a hydrophilic synthetic analogue of α-tocopherol, which is widely used as a standard antioxidant. The present study demonstrated that isopentyl ferulate has an antioxidant activity in vitro experimental models, suggesting that this compound could enhance the development of a new product with antioxidant properties. However, further in vivo studies are needed to assign possible implications in the treatment of diseases related with free radicals.
authors.affiliation: Post-Graduation Program in Pharmacology, Federal University of Piauí, CEP 64.049-550 Teresina, Piauí, Brazil.
authors.forename: Keylla C
authors.initials: KC
authors.lastname: Machado
authors.affiliation: Laboratory of Research in Experimental Neurochemistry of Post-Graduation Program in Pharmaceutical Sciences, Federal University of Piauí, CEP 64.049-550 Teresina, Piauí, Brazil.
authors.forename: George Laylson S
authors.initials: GL
authors.lastname: Oliveira
authors.affiliation: Department of Pharmaceutical Science, Federal University of Paraíba, CEP 58.051-900 João Pessoa, Paraíba, Brazil.
authors.forename: Élida B V
authors.initials: ÉB
authors.lastname: de Sousa
authors.affiliation: Laboratory of Research in Experimental Neurochemistry of Post-Graduation Program in Pharmaceutical Sciences, Federal University of Piauí, CEP 64.049-550 Teresina, Piauí, Brazil.
authors.forename: Iwyson Henrique F
authors.initials: IH
authors.lastname: Costa
authors.affiliation: Laboratory of Research in Experimental Neurochemistry of Post-Graduation Program in Pharmaceutical Sciences, Federal University of Piauí, CEP 64.049-550 Teresina, Piauí, Brazil.
authors.forename: Katia C
authors.initials: KC
authors.lastname: Machado
authors.affiliation: Department of Pharmaceutical Science, Federal University of Paraíba, CEP 58.051-900 João Pessoa, Paraíba, Brazil.
authors.forename: Damião Pergentino
authors.initials: DP
authors.lastname: de Sousa
authors.affiliation: Department of Chemistry, University of Alabama in Huntsville, AL 35899, USA.
authors.forename: Prabodh
authors.initials: P
authors.lastname: Satyal
authors.affiliation: Post-Graduation Program in Pharmacology, Federal University of Piauí, CEP 64.049-550 Teresina, Piauí, Brazil; Laboratory of Research in Experimental Neurochemistry of Post-Graduation Program in Pharmaceutical Sciences, Federal University of Piauí, CEP 64.049-550 Teresina, Piauí, Brazil. Electronic address: rivelilson@pq.cnpq.br.
authors.forename: Rivelilson Mendes
authors.initials: RM
authors.lastname: de Freitas
mesh_terms: D052160:Benzothiazoles / Q000737:chemistry; D001713:Biphenyl Compounds / Q000737:chemistry; D003373:Coumaric Acids / Q000737:chemistry*; D016166:Free Radical Scavengers / Q000737:chemistry*; D017665:Hydroxyl Radical / Q000737:chemistry; D009569:Nitric Oxide / Q000737:chemistry; D010084:Oxidation-Reduction; D010851:Picrates / Q000737:chemistry; D013451:Sulfonic Acids / Q000737:chemistry; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis
chemical_list: D052160:Benzothiazoles; D001713:Biphenyl Compounds; D003373:Coumaric Acids; D016166:Free Radical Scavengers; D010851:Picrates; D013451:Sulfonic Acids; D017392:Thiobarbituric Acid Reactive Substances; C002502:2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid; D009569:Nitric Oxide; D017665:Hydroxyl Radical; C004999:ferulic acid; C004931:1,1-diphenyl-2-picrylhydrazyl
keywords: Antioxidant; Free radicals; Hydroxyl; Nitric oxide; Phenylpropanoids