title: Visinin-like peptide 1 in adrenal gland of the rat. Gene expression and its hormonal control.
abstract: VSNL1 encodes the calcium-sensor protein visinin-like 1 and was identified previously as an upregulated gene in a sample set of aldosterone-producing adenomas. Recently, by means of microarray studies we demonstrated high expression of Vsnl1 gene in rat adrenal zona glomerulosa (ZG). Only scanty data are available on the role of this gene in adrenal function as well as on regulation of its expression by factors affecting adrenal cortex structure and function. Therefore we performed relevant studies aimed at clarifying some of the above issues. By Affymetrix(®) Rat Gene 1.1 ST Array Strip, QPCR and immunohistochemistry we demonstrated that expression levels of Vsnl1 in the rat adrenal ZG are notably higher than in the fasciculata/reticularis zone. In QPCR assay this difference was approximately 10 times higher. Expression of this gene in the rat adrenal gland or adrenocortical cells was acutely down regulated by ACTH, while chronic administration of corticotrophin or dexamethasone did not change Vsnl1 mRNA levels. In enucleation-induced adrenocortical regeneration expression levels of both Vsnl1 and Cyp11b2 were notably lowered and positively correlated. Despite these findings, the physiological significance of adrenal Vsnl1 remains unclear, and requires further investigation.
authors.affiliation: Department of Histology and Embryology, Poznań University of Medical Sciences, Poznań, Poland.
authors.forename: Marcin
authors.initials: M
authors.lastname: Trejter
authors.affiliation: Department of Histology and Embryology, Poznań University of Medical Sciences, Poznań, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Hochol
authors.affiliation: Department of Histology and Embryology, Poznań University of Medical Sciences, Poznań, Poland.
authors.forename: Marianna
authors.initials: M
authors.lastname: Tyczewska
authors.affiliation: Department of Histology and Embryology, Poznań University of Medical Sciences, Poznań, Poland.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Ziolkowska
authors.affiliation: Department of Histology and Embryology, Poznań University of Medical Sciences, Poznań, Poland.
authors.forename: Karol
authors.initials: K
authors.lastname: Jopek
authors.affiliation: Department of Histology and Embryology, Poznań University of Medical Sciences, Poznań, Poland.
authors.forename: Marta
authors.initials: M
authors.lastname: Szyszka
authors.affiliation: Department of Histology and Embryology, Poznań University of Medical Sciences, Poznań, Poland. Electronic address: lkm@amp.edu.pl.
authors.forename: Ludwik K
authors.initials: LK
authors.lastname: Malendowicz
authors.affiliation: Department of Histology and Embryology, Poznań University of Medical Sciences, Poznań, Poland.
authors.forename: Marcin
authors.initials: M
authors.lastname: Rucinski
mesh_terms: D000311:Adrenal Glands / Q000378:metabolism*; D000324:Adrenocorticotropic Hormone / Q000494:pharmacology / Q000502:physiology; D000818:Animals; D003907:Dexamethasone / Q000494:pharmacology; D005260:Female; D015870:Gene Expression; D005938:Glucocorticoids / Q000494:pharmacology; D008297:Male; D051596:Neurocalcin / Q000235:genetics / Q000378:metabolism*; D017208:Rats, Wistar
chemical_list: D005938:Glucocorticoids; D051596:Neurocalcin; C496690:Vsnl1 protein, rat; D003907:Dexamethasone; D000324:Adrenocorticotropic Hormone
keywords: Adrenal; Adrenal regeneration; Gene expression; Immunocytochemistry; Microarray; Visinin-like peptide 1