title: Fate and biotransformation of phytosterols during treatment of pulp and paper wastewater in a simulated aerated stabilization basin.
abstract: Pulp and paper wastewater (PPW) contains significant concentrations of phytosterols, suspected of inducing endocrine disruption in aquatic species. Aerated stabilization basins (ASBs) are commonly used for the treatment of PPW, but phytosterol removal varies among treatment systems. The objective of this study was to better understand the removal processes and biotransformation of phytosterols within an ASB treatment system fed with untreated PPW. PPW settled solids and supernatant fractions showed that phytosterols are primarily associated with settleable solids, which carry phytosterols to ASB sediment where anoxic/anaerobic conditions prevail. Bioassays with supernatant and settled PPW fractions of the raw wastewater conducted under aerobic and anaerobic conditions, respectively, showed that solids disintegration and hydrolysis results in phytosterol release in ASBs. A simulated ASB, fed with PPW and operated for 2.4 years at three hydraulic retention times (HRTs; 22.2, 11.1 and 5.6 d) with total phytosterol and solids loading rates from 10 to 42 μg/L-d and 44-178 mg/L-d, respectively, was used to determine the steady-state effluent quality and sediment characteristics. Although effluent COD and phytosterol concentrations were relatively low and stable (84-88% total COD removal; 82-94% total phytosterol removal) across the range of HRTs tested, sediment COD and phytosterol concentrations increased with increasing loading rate. On average, 51% of the phytosterols entering the ASB were removed via biotransformation, 40% were retained in the sediment, and the remaining 9% exited with the effluent. This study demonstrates the role of sediment as a source of phytosterol release in ASBs and highlights the importance of HRT and the PPW characteristics for predicting phytosterol fate in ASBs.
authors.affiliation: School of Civil and Environmental Engineering, Georgia Institute of Technology, 311 Ferst Drive, Atlanta, GA 30332-0512, USA.
authors.forename: Christy M
authors.initials: CM
authors.lastname: Dykstra
authors.forename: Hamilton D
authors.initials: HD
authors.lastname: Giles
authors.forename: Sujit
authors.initials: S
authors.lastname: Banerjee
authors.forename: Spyros G
authors.initials: SG
authors.lastname: Pavlostathis
mesh_terms: D000332:Aerobiosis; D000693:Anaerobiosis; D001419:Bacteria / Q000378:metabolism; D001711:Biotransformation; D006863:Hydrogen-Ion Concentration; D007220:Industrial Waste; D015394:Molecular Structure; D010209:Paper; D010840:Phytosterols / Q000737:chemistry / Q000378:metabolism*; D013997:Time Factors; D014865:Waste Disposal, Fluid / Q000379:methods*; D062065:Wastewater / Q000737:chemistry / Q000382:microbiology; D014871:Water Microbiology
chemical_list: D007220:Industrial Waste; D010840:Phytosterols; D062065:Wastewater