title: Fabrication of PLGA/MWNTs composite electrospun fibrous scaffolds for improved myogenic differentiation of C2C12 cells.
abstract: Electrically conducting scaffolds have attracted tremendous attention in skeletal muscle tissue engineering. In this paper, poly(lactic-co-glycolic acid) (PLGA)/multi-wall carbon nanotubes (MWNTs) composite fibrous scaffolds were fabricated using the electrospinning technique. The physical properties of the composite fibers were characterized and proliferation and differentiation of C2C12 cells on these scaffolds were examined. It was found that the addition of MWNTs modulated the physical properties of PLGA fibers including morphology, fiber diameter, degradation, tensile strength and electrical conductivity, depending on the amount of MWNTs. These fibrous scaffolds were cytocompatible and supported the proliferation of C2C12 cells. Importantly, C2C12 cells showed more mature myotube formation on PLGA/MWNTs composite fibrous scaffolds compared to PLGA scaffolds. These results indicate that PLGA/MWNTs composite electrospun fibers have great potential in skeletal muscle tissue engineering.
authors.affiliation: State Key Laboratory of Bioreactor Engineering, School of Bioengineering, East China University of Science and Technology, 130 Mei-Long Road, P. O. Box 309 Shanghai 200237, PR China. Electronic address: xujz2009@126.com.
authors.forename: Jiazhu
authors.initials: J
authors.lastname: Xu
authors.affiliation: Complex and Intelligent Research Centre, School of Mechanical and Power Engineering, East China University of Science and Technology, 130 Mei-Long Road, P. O. Box 401 Shanghai 200237, PR China. Electronic address: hyxieya@163.com.
authors.forename: Ya
authors.initials: Y
authors.lastname: Xie
authors.affiliation: Complex and Intelligent Research Centre, School of Mechanical and Power Engineering, East China University of Science and Technology, 130 Mei-Long Road, P. O. Box 401 Shanghai 200237, PR China. Electronic address: hbzhang@ecust.edu.cn.
authors.forename: Hongbo
authors.initials: H
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Bioreactor Engineering, School of Bioengineering, East China University of Science and Technology, 130 Mei-Long Road, P. O. Box 309 Shanghai 200237, PR China. Electronic address: zhaoyangye@ecust.edu.cn.
authors.forename: Zhaoyang
authors.initials: Z
authors.lastname: Ye
authors.affiliation: Complex and Intelligent Research Centre, School of Mechanical and Power Engineering, East China University of Science and Technology, 130 Mei-Long Road, P. O. Box 401 Shanghai 200237, PR China; Department of Biomedical Engineering, University of Saskatchewan, Canada. Electronic address: zhangwjchris@gmail.com.
authors.forename: Wenjun
authors.initials: W
authors.lastname: Zhang
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000502:physiology; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D019344:Lactic Acid / Q000737:chemistry*; D051379:Mice; D018482:Muscle, Skeletal / Q000166:cytology; D037742:Nanotubes, Carbon / Q000737:chemistry*; D011100:Polyglycolic Acid / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D023822:Tissue Engineering / Q000379:methods; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D037742:Nanotubes, Carbon; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid
keywords: C2C12 cells; Carbon nanotubes; Electrical conductivity; Electrospinning; Poly(lactic-co-glycolic acid); Skeletal muscle tissue engineering