title: Biodiesel production by combined fatty acids separation and subsequently enzymatic esterification to improve the low temperature properties.
abstract: The poor low-temperature properties of biodiesel, which provokes easy crystallization at low temperature, can cause fuel line plugging and limits its blending amount with petro-diesel. This work aimed to study the production of biodiesel with a new process of improving the low temperature performance of biodiesel. Waste cooking oil was first hydrolyzed into fatty acids (FAs) by 60g immobilized lipase and 240g RO water in 15h. Then, urea complexation was used to divide the FAs into saturated and unsaturated components. The conditions for complexation were: FA-to-urea ratio 1:2 (w/w), methanol to FA ratio 5:1 (v/v), duration 2h. The saturated and unsaturated FAs were then converted to iso-propyl and methyl esters by lipase, respectively. Finally, the esters were mixed together. The CFPP of this mixture was decreased from 5°C to -3°C. Hydrolysis, urea complexation and enzymic catalyzed esterification processes are discussed in this paper.
authors.affiliation: Beijing Bioprocess Key Laboratory, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, PR China.
authors.forename: Meng
authors.initials: M
authors.lastname: Wang
authors.affiliation: Beijing Bioprocess Key Laboratory, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, PR China; Amoy - BUCT Industrial Bio-Technovation Institute, Amoy 361022, PR China.
authors.forename: Kaili
authors.initials: K
authors.lastname: Nie
authors.affiliation: Beijing Bioprocess Key Laboratory, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, PR China.
authors.forename: Hao
authors.initials: H
authors.lastname: Cao
authors.affiliation: Beijing Bioprocess Key Laboratory, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, PR China; Amoy - BUCT Industrial Bio-Technovation Institute, Amoy 361022, PR China. Electronic address: dengli@mail.buct.edu.cn.
authors.forename: Li
authors.initials: L
authors.lastname: Deng
authors.affiliation: Beijing Bioprocess Key Laboratory, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, PR China; State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing 100029, PR China.
authors.forename: Fang
authors.initials: F
authors.lastname: Wang
authors.affiliation: Beijing Bioprocess Key Laboratory, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, PR China.
authors.forename: Tianwei
authors.initials: T
authors.lastname: Tan
mesh_terms: D019840:2-Propanol / Q000378:metabolism; D055162:Biocatalysis; D056804:Biofuels; D001709:Biotechnology / Q000379:methods*; D002175:Candida / Q000201:enzymology; D003080:Cold Temperature; D004951:Esterification; D004952:Esters / Q000378:metabolism; D005227:Fatty Acids / Q000302:isolation & purification*; D005374:Filtration / Q000295:instrumentation; D006868:Hydrolysis; D008049:Lipase / Q000378:metabolism*; D000432:Methanol / Q000378:metabolism; D014508:Urea / Q000378:metabolism
chemical_list: D056804:Biofuels; D004952:Esters; D005227:Fatty Acids; D014508:Urea; D008049:Lipase; D019840:2-Propanol; D000432:Methanol
keywords: Biodiesel; Cold filter plugging point; Esterification; Hydrolysis; Urea complexation