title: [Association between -1296T/C and -915A/G polymorphisms of matrix metalloproteinase inhibitor-3 gene and atherosclerotic cerebral infarction in an ethnic Han Chinese population].
abstract: OBJECTIVE
  To assess the association between -1296T/C and -915A/G polymorphisms in the promoter region of matrix metalloproteinase inhibitor-3 gene (TIMP-3) and atherosclerotic cerebral infarction in an ethnic Han Chinese population.
  
  
  METHODS
  Peripheral blood samples were collected from 485 patients with atherosclerotic cerebral infarction and 525 healthy controls. Serum levels of TIMP-3 were measured with an enzyme-linked immunosorbent assay (ELISA). The polymorphisms of the TIMP-3 gene were analyzed with DNA sequencing.
  
  
  RESULTS
  There were significant differences in genotype and allele frequencies in -1296T/C and -915A/G between the patients and healthy controls (chi-square: 5.227 and 5.869; P: 0.022 and 0.015, respectively). Besides, there was a strong linkage disequilibrium between -1296T/C and -915A/G (D'=1.0, r(2)=0.991). The serum levels of TIMP-3 in patients were significantly higher than the control group [(248.90 ± 97.10) pg/mL vs. (200.17 ± 79.70) pg/mL, t=2.098, P=0.039].
  
  
  CONCLUSIONS
  The -1296T/C and -915A/G polymorphisms of the TIMP-3 gene are associated with increased risk for atherosclerotic cerebral infarction in ethnic Han Chinese and may be used as molecular markers for the disease. There is also strong linkage disequilibrium between the two loci.
authors.affiliation: Department of Neurology, Taizhou Hospital Affiliated to Wenzhou Medical College, Taizhou, Zhejiang 317000, P. R. China. jinxp155@aliyun.com.
authors.forename: Zhou
authors.initials: Z
authors.lastname: Zheng
authors.forename: Xiaoping
authors.initials: X
authors.lastname: Jin
authors.forename: Min
authors.initials: M
authors.lastname: Zhu
authors.forename: Cai
authors.initials: C
authors.lastname: Li
authors.forename: Feng
authors.initials: F
authors.lastname: Zhu
authors.forename: Weiling
authors.initials: W
authors.lastname: Li
authors.forename: Qing
authors.initials: Q
authors.lastname: Lin
authors.forename: Feng
authors.initials: F
authors.lastname: Wang
authors.forename: Wanfen
authors.initials: W
authors.lastname: Wang
authors.forename: Xiaofei
authors.initials: X
authors.lastname: Hu
authors.forename: Chenling
authors.initials: C
authors.lastname: Lyu
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D044466:Asian People / Q000208:ethnology / Q000235:genetics; D050197:Atherosclerosis / Q000097:blood / Q000453:epidemiology / Q000208:ethnology / Q000235:genetics*; D001483:Base Sequence; D002544:Cerebral Infarction / Q000097:blood / Q000453:epidemiology / Q000208:ethnology / Q000235:genetics*; D002681:China / Q000453:epidemiology; D005260:Female; D005787:Gene Frequency; D006801:Humans; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D020641:Polymorphism, Single Nucleotide; D012307:Risk Factors; D019717:Tissue Inhibitor of Metalloproteinase-3 / Q000097:blood / Q000235:genetics*
chemical_list: C088390:TIMP3 protein, human; D019717:Tissue Inhibitor of Metalloproteinase-3