title: Single-walled carbon nanotubes as delivery vehicles enhance the immunoprotective effects of a recombinant vaccine against Aeromonas hydrophila.
abstract: To reduce the economic losses caused by diseases in aquaculture industry, more efficient and economic prophylactic measures should be urgently investigated. In this research, the effects of a novel functionalized single-walled carbon nanotubes (SWCNTs) applied as a delivery vehicle for recombinant Aeromonas hydrophila vaccine administration via bath or injection in juvenile grass carp were studied. The results showed that SWCNT as a vector for the recombinant protein aerA, augmented the production of specific antibodies, apparently stimulated the induction of immune-related genes, and induced higher level of survival rate compared with free aerA subunit vaccine. Furthermore, we compared the routes of bath and intramuscular injection immunization by SWCNTs-aerA vaccine, and found that similar antibody levels induced by SWCNTs-aerA were observed in both immunization routes. Meanwhile, a similar relative percentage survival (approximately 80%) was found in both a 40 mg/L bath immunization group, and a 20 μg injection group. The results indicate that functionalized SWCNTs could be a promising delivery vehicle to potentiate the immune response of recombinant vaccines, and might be used to vaccinate juvenile fish by bath administration method.
authors.affiliation: Northwest A&F University, Yangling, 712100, People's Republic of China.
authors.forename: Yu-Xin
authors.initials: YX
authors.lastname: Gong
authors.affiliation: Northwest A&F University, Yangling, 712100, People's Republic of China.
authors.forename: Bin
authors.initials: B
authors.lastname: Zhu
authors.affiliation: Northwest A&F University, Yangling, 712100, People's Republic of China.
authors.forename: Guang-Lu
authors.initials: GL
authors.lastname: Liu
authors.affiliation: Northwest A&F University, Yangling, 712100, People's Republic of China.
authors.forename: Lei
authors.initials: L
authors.lastname: Liu
authors.affiliation: Northwest A&F University, Yangling, 712100, People's Republic of China.
authors.forename: Fei
authors.initials: F
authors.lastname: Ling
authors.affiliation: Northwest A&F University, Yangling, 712100, People's Republic of China. Electronic address: wanggaoxue@126.com.
authors.forename: Gao-Xue
authors.initials: GX
authors.lastname: Wang
authors.affiliation: Northwest A&F University, Yangling, 712100, People's Republic of China. Electronic address: 286567031@qq.com.
authors.forename: Xin-Gang
authors.initials: XG
authors.lastname: Xu
mesh_terms: D016980:Aeromonas hydrophila / Q000276:immunology*; D000704:Analysis of Variance; D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology; D001428:Bacterial Vaccines / Q000008:administration & dosage / Q000494:pharmacology*; D002347:Carps; D017931:DNA Primers / Q000235:genetics; D016503:Drug Delivery Systems / Q000379:methods / Q000662:veterinary; D004797:Enzyme-Linked Immunosorbent Assay; D005393:Fish Diseases / Q000382:microbiology* / Q000517:prevention & control*; D016905:Gram-Negative Bacterial Infections / Q000517:prevention & control / Q000662:veterinary*; D037742:Nanotubes, Carbon; D060888:Real-Time Polymerase Chain Reaction; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000494:pharmacology
chemical_list: D000907:Antibodies, Bacterial; D001428:Bacterial Vaccines; D017931:DNA Primers; D037742:Nanotubes, Carbon; D014614:Vaccines, Synthetic
keywords: Aeromonas hydrophila; Bath immunization; Immune response; Single-walled carbon nanotubes; Vaccine