title: Nasal immunization of mice with AFCo1 or AFPL1 plus capsular polysaccharide Vi from Salmonella typhi induces cellular response and memory B and T cell responses.
abstract: The response to infection against Salmonella involves both B and T cell mediated immunity. An effective immunization can activate an adequate immune response capable to control the primary infection and protect against a secondary infection. Mucosal vaccination, by inducing local pathogen-specific immune responses, has the potential to counter mucosally transmitted pathogens at the portal of entry, thereby increasing the efficacy of vaccines. The aim of this work was to explore the efficacy of AFCo1 or AFPL1, as mucosal adjuvants to stimulate cell immunity and memory responses against Vi polysaccharide antigen of Salmonella typhi (PsVi). Mice immunized with 3 intranasal doses exhibited high levels of PsVi-specific IgG (p<0.05), IgG2a and IgG2c subclasses. Also, an amplified recall response after a booster immunization with a plain polysaccharide vaccine was induced. Avidities index were higher in mice immunized with adjuvanted formulations at different chaotropic concentrations. Furthermore, IL-12 and IFN-γ levels in nasally vaccinated mice with both adjuvants were induced. Moreover, priming with 3 doses followed by booster immunization with VaxTyVi(®) resulted in high levels of anti-Vi specific IgG, IgG subclasses and antibody avidity. Long lived plasma cells in bone marrow, memory B cells and long-term memory T cells after booster dose were induced. The combined formulation of Vi polysaccharide with mucosal adjuvants provides an improved immunogenicity, in particular with regard to cellular responses and long lasting cells responses.
authors.affiliation: Immunology Department, Finlay Institute, Havana, Cuba. Electronic address: belkisorama@gmail.com.
authors.forename: Belkis
authors.initials: B
authors.lastname: Romeu
authors.affiliation: Immunology Department, Finlay Institute, Havana, Cuba.
authors.forename: Miriam
authors.initials: M
authors.lastname: Lastre
authors.affiliation: Immunology Department, Finlay Institute, Havana, Cuba.
authors.forename: Laura
authors.initials: L
authors.lastname: Reyes
authors.affiliation: Immunology Department, Finlay Institute, Havana, Cuba.
authors.forename: Elizabeth
authors.initials: E
authors.lastname: González
authors.affiliation: Immunology Department, Finlay Institute, Havana, Cuba.
authors.forename: Yusnaby
authors.initials: Y
authors.lastname: Borrero
authors.affiliation: Immunology Department, Finlay Institute, Havana, Cuba.
authors.forename: Diandra
authors.initials: D
authors.lastname: Lescaille
authors.affiliation: Immunology Department, Finlay Institute, Havana, Cuba.
authors.forename: Rocmira
authors.initials: R
authors.lastname: Pérez
authors.affiliation: Animal Models Group, Direction of Research and Development, Finlay Institute, Havana, Cuba.
authors.forename: Darzy
authors.initials: D
authors.lastname: Nuñez
authors.affiliation: Immunology Department, Finlay Institute, Havana, Cuba.
authors.forename: Oliver
authors.initials: O
authors.lastname: Pérez
mesh_terms: D000276:Adjuvants, Immunologic / Q000008:administration & dosage*; D000281:Administration, Intranasal; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D000915:Antibody Affinity; D001402:B-Lymphocytes / Q000276:immunology*; D005260:Female; D007111:Immunity, Cellular; D007114:Immunization / Q000379:methods; D007074:Immunoglobulin G / Q000097:blood; D007156:Immunologic Memory; D051379:Mice; D008810:Mice, Inbred C57BL; D011135:Polysaccharides, Bacterial / Q000008:administration & dosage / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*; D014436:Typhoid-Paratyphoid Vaccines / Q000008:administration & dosage / Q000276:immunology*
chemical_list: D000276:Adjuvants, Immunologic; D000907:Antibodies, Bacterial; D007074:Immunoglobulin G; D011135:Polysaccharides, Bacterial; D014436:Typhoid-Paratyphoid Vaccines; C054154:capsular polysaccharide, Salmonella
keywords: Adjuvants; Cellular responses; Cochleates; Proteoliposomes; Vi polysaccharide