title: Starving/re-feeding processes induce metabolic modifications in thick-lipped grey mullet (Chelon labrosus, Risso 1827).
abstract: The effects of starvation and re-feeding on metabolites and tissue composition, GH/IGF-I axis, and digestive enzyme activities in juvenile thick-lipped grey mullet (Chelon labrosus) were evaluated. Fish were divided into three feeding groups (n=72, 82.00±4.09 g initial body mass). The control group was fed 1% of their body mass once a day throughout the experiment with commercial pellets. The other two groups were deprived of feed for 21 days (starved), or re-fed for 7 days after 14 days of food deprivation (re-fed). Full-length cDNAs from pituitary GH and hepatic IGF-I were cloned by screening a cDNA library or by PCR techniques. Furthermore, changes in their mRNA expressions were assessed by real time PCR in specimens maintained under the different feeding patterns. Results showed a negative growth in starved and re-feeding groups. Starvation induced a significant increase in plasma triglycerides as well as a decrease in liver glucose and glycogen. Re-feeding increased plasma glucose, lactate and protein, as well as liver glucose and glycogen. In addition, starvation significantly increased pituitary GH expression, while re-feeding down-regulated it. No significant changes were observed in hepatic IGF-I expression in any dietary treatment. Digestive enzyme activities were not significantly affected either by starvation or by re-feeding. The results of the present work suggest that juveniles of the thick-lipped grey mullet may easily adjust their metabolism under situations characterized by a short-term starvation and re-feeding.
authors.affiliation: Departamento de Biología, Facultad de Ciencias del Mar y Ambientales, Campus de Excelencia Internacional del Mar (CEI-MAR), Universidad de Cádiz, 11510 Puerto Real, Cádiz, Spain. Electronic address: isabel.pujante@uca.es.
authors.forename: I M
authors.initials: IM
authors.lastname: Pujante
authors.affiliation: Instituto de Ciencias Marinas de Andalucía (ICMAN), Campus de Excelencia Internacional del Mar (CEI-MAR), Consejo Superior de Investigaciones Científicas (CSIC), 11510 Puerto Real, Cádiz, Spain.
authors.forename: J A
authors.initials: JA
authors.lastname: Martos-Sitcha
authors.affiliation: Departamento de Biología Aplicada, Escuela Politécnica, Campus de Excelencia Internacional del Mar (CEI-MAR), Universidad de Almería, 04120, La Cañada de San Urbano, Almería, Spain.
authors.forename: F J
authors.initials: FJ
authors.lastname: Moyano
authors.affiliation: Departamento de Biología, Facultad de Ciencias del Mar y Ambientales, Campus de Excelencia Internacional del Mar (CEI-MAR), Universidad de Cádiz, 11510 Puerto Real, Cádiz, Spain.
authors.forename: I
authors.initials: I
authors.lastname: Ruiz-Jarabo
authors.affiliation: Instituto de Ciencias Marinas de Andalucía (ICMAN), Campus de Excelencia Internacional del Mar (CEI-MAR), Consejo Superior de Investigaciones Científicas (CSIC), 11510 Puerto Real, Cádiz, Spain.
authors.forename: G
authors.initials: G
authors.lastname: Martínez-Rodríguez
authors.affiliation: Departamento de Biología, Facultad de Ciencias del Mar y Ambientales, Campus de Excelencia Internacional del Mar (CEI-MAR), Universidad de Cádiz, 11510 Puerto Real, Cádiz, Spain.
authors.forename: J M
authors.initials: JM
authors.lastname: Mancera
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D004064:Digestive System / Q000201:enzymology; D004734:Energy Metabolism; D013006:Growth Hormone / Q000378:metabolism; D007334:Insulin-Like Growth Factor I / Q000378:metabolism; D008969:Molecular Sequence Data; D009928:Organ Specificity; D012333:RNA, Messenger / Q000378:metabolism; D023701:Smegmamorpha / Q000378:metabolism*; D013217:Starvation
chemical_list: D012333:RNA, Messenger; D007334:Insulin-Like Growth Factor I; D013006:Growth Hormone
keywords: Chelon labrosus; Food deprivation; GH/IGF-I system; Metabolism