title: Trpac1, a pH response transcription regulator, is involved in cellulase gene expression in Trichoderma reesei.
abstract: Fungi grow over a relatively wide pH range and adapt to extracellular pH through a genetic regulatory system mediated by a key component PacC, which is a pH transcription regulator. The cellulase production of the filamentous fungi Trichoderma reesei is sensitive to ambient pH. To investigate the connection between cellulase expression regulation and ambient pH, an ortholog of Aspergillus nidulans pacC, Trpac1, was identified and functionally characterized using a target gene deletion strategy. Deleting Trpac1 dramatically increased the cellulase production and the transcription levels of the major cellulase genes at neutral pH, which suggested Trpac1 is involved in the regulation of cellulase production. It was further observed that the expression levels of transcription factors xyr1 and ace2 also increased in the ΔTrpac1 mutant at neutral pH. In addition, the ΔTrpac1 mutant exhibited conidiation defects under neutral and alkaline pH. These results implied that Trpac1 in involved in growth and development process and cellulase gene expression in T. reesei.
authors.affiliation: Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, 32 West 7th Avenue, Tianjin Airport Economic Area, Tianjin 300308, China. Electronic address: he_rl@tib.cas.cn.
authors.forename: Ronglin
authors.initials: R
authors.lastname: He
authors.affiliation: College of Biotechnology, Tianjin University of Science and Technology, 13th Avenue, Tianjin Economic and Technological Development Area (TEDA), Tianjin 300457, China. Electronic address: malj@tust.edu.cn.
authors.forename: Lijuan
authors.initials: L
authors.lastname: Ma
authors.affiliation: Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, 32 West 7th Avenue, Tianjin Airport Economic Area, Tianjin 300308, China. Electronic address: li_c@tib.cas.cn.
authors.forename: Chen
authors.initials: C
authors.lastname: Li
authors.affiliation: Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, 32 West 7th Avenue, Tianjin Airport Economic Area, Tianjin 300308, China. Electronic address: jia_wd@tib.cas.cn.
authors.forename: Wendi
authors.initials: W
authors.lastname: Jia
authors.affiliation: Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, 32 West 7th Avenue, Tianjin Airport Economic Area, Tianjin 300308, China. Electronic address: li_dm@tib.cas.cn.
authors.forename: Demao
authors.initials: D
authors.lastname: Li
authors.affiliation: Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, 32 West 7th Avenue, Tianjin Airport Economic Area, Tianjin 300308, China. Electronic address: zhang_dy@tib.cas.cn.
authors.forename: Dongyuan
authors.initials: D
authors.lastname: Zhang
authors.affiliation: Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, 32 West 7th Avenue, Tianjin Airport Economic Area, Tianjin 300308, China. Electronic address: chen_sl@tib.cas.cn.
authors.forename: Shulin
authors.initials: S
authors.lastname: Chen
mesh_terms: D000595:Amino Acid Sequence; D002480:Cellulase / Q000096:biosynthesis / Q000235:genetics*; D005656:Fungal Proteins / Q000096:biosynthesis / Q000235:genetics*; D017353:Gene Deletion; D018507:Gene Expression Regulation, Developmental; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal; D005816:Genetic Complementation Test; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D009154:Mutation; D010802:Phylogeny; D017386:Sequence Homology, Amino Acid; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism; D014242:Trichoderma / Q000201:enzymology* / Q000235:genetics* / Q000254:growth & development
chemical_list: D005656:Fungal Proteins; D014157:Transcription Factors; D002480:Cellulase
keywords: Cellulase regulation; Transcription factors; Trichoderma reesei; Trpac1; pH