title: Differential regulation of hepatopancreatic vitellogenin (VTG) gene expression by two putative molt-inhibiting hormones (MIH1/2) in Pacific white shrimp (Litopenaeus vannamei).
abstract: Molt-inhibiting hormone (MIH), a peptide member of the crustacean hyperglycemic hormone (CHH) family, is commonly considered as a negative regulator during the molt cycle in crustaceans. Phylogenetic analysis of CHH family peptides in penaeidae shrimps suggested that there is no significant differentiation between MIH and vitellogenesis-inhibiting hormone (VIH, another peptide member of CHH family), by far the most potent negative regulator of crustacean vitellogenesis known. Thus, MIH may also play a role in regulating vitellogenesis. In this study, two previously reported putative MIHs (LivMIH1 and LivMIH2) in the Pacific white shrimp (Litopenaeus vannamei) were expressed in Escherichia coli, purified by immobilized metal ion affinity chromatography (IMAC) and further confirmed by western blot. Regulation of vitellogenin (VTG) mRNA expression by recombinant LivMIH1 and LivMIH2 challenge was performed by both in vitro hepatopancreatic primary cells culture and in vivo injection approaches. In in vitro primary culture of shrimp hepatopancreatic cells, only LivMIH2 but not LivMIH1 administration could improve the mRNA expression of VTG. In in vivo injection experiments, similarly, only LivMIH2 but not LivMIH1 could stimulate hepatopancreatic VTG gene expression and induce ovary maturation. Our study may provide evidence for one isoform of MIH (MIH2 in L. vannamei) may serve as one of the mediators of the physiological progress of molting and vitellogenesis. Our study may also give new insight in CHH family peptides regulating reproduction in crustaceans, in particular penaeidae shrimps.
authors.affiliation: CAS Key Laboratory of Tropical Marine Bio-resources and Ecology (LMB), Guangdong Provincial Key Laboratory of Applied Marine Biology (LAMB), South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, China; University of Chinese Academy of Sciences, Beijing, China. Electronic address: luoxingzky@163.com.
authors.forename: Xing
authors.initials: X
authors.lastname: Luo
authors.affiliation: CAS Key Laboratory of Tropical Marine Bio-resources and Ecology (LMB), Guangdong Provincial Key Laboratory of Applied Marine Biology (LAMB), South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, China. Electronic address: chan1010@scsio.ac.cn.
authors.forename: Ting
authors.initials: T
authors.lastname: Chen
authors.affiliation: CAS Key Laboratory of Tropical Marine Bio-resources and Ecology (LMB), Guangdong Provincial Key Laboratory of Applied Marine Biology (LAMB), South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, China; University of Chinese Academy of Sciences, Beijing, China. Electronic address: michell.mzhong@hotmail.com.
authors.forename: Ming
authors.initials: M
authors.lastname: Zhong
authors.affiliation: CAS Key Laboratory of Tropical Marine Bio-resources and Ecology (LMB), Guangdong Provincial Key Laboratory of Applied Marine Biology (LAMB), South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, China. Electronic address: jiangxiao4367@126.com.
authors.forename: Xiao
authors.initials: X
authors.lastname: Jiang
authors.affiliation: CAS Key Laboratory of Tropical Marine Bio-resources and Ecology (LMB), Guangdong Provincial Key Laboratory of Applied Marine Biology (LAMB), South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, China. Electronic address: lpzhang@scsio.ac.cn.
authors.forename: Lvping
authors.initials: L
authors.lastname: Zhang
authors.affiliation: CAS Key Laboratory of Tropical Marine Bio-resources and Ecology (LMB), Guangdong Provincial Key Laboratory of Applied Marine Biology (LAMB), South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, China. Electronic address: rosemary166@sina.com.
authors.forename: Chunhua
authors.initials: C
authors.lastname: Ren
authors.affiliation: CAS Key Laboratory of Tropical Marine Bio-resources and Ecology (LMB), Guangdong Provincial Key Laboratory of Applied Marine Biology (LAMB), South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, China. Electronic address: cqhu@scsio.ac.cn.
authors.forename: Chaoqun
authors.initials: C
authors.lastname: Hu
mesh_terms: D000818:Animals; D005260:Female; D015870:Gene Expression; D005786:Gene Expression Regulation; D043143:Hepatopancreas / Q000378:metabolism*; D007447:Invertebrate Hormones / Q000235:genetics / Q000378:metabolism*; D018978:Molting; D009928:Organ Specificity; D010053:Ovary / Q000254:growth & development / Q000378:metabolism; D033561:Penaeidae / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D014818:Vitellogenesis; D014819:Vitellogenins / Q000235:genetics / Q000378:metabolism*
chemical_list: D007447:Invertebrate Hormones; D014819:Vitellogenins
keywords: Hepatopancreas; Litopenaeus vannamei; MIH; Reproduction; VIH; Vitellogenesis