title: Ethanolic extract of Coelogyne cristata Lindley (Orchidaceae) and its compound coelogin promote osteoprotective activity in ovariectomized estrogen deficient mice.
abstract: Coelogyne cristata Lindley (CC) family Orchidaceae is an Indian medicinal plant used for the treatment of fractured bones in folk-tradition of Kumaon region, Uttarakhand, India. In continuation of our drug discovery program, feeding of ethanolic extract to ovariectomized estrogen deficient mice led to significant restoration of trabecular micro architecture in both femoral and tibial bones, better bone quality and also devoid of any uterine estrogenicity. Subsequently, coelogin, a pure compound was isolated from ethyl acetate fraction of C. cristata and evaluated in in vitro osteoblast cell cultures. Treatment of coelogin to osteoblasts led to enhanced ALP activity (a marker of osteoblast differentiation), mineral nodule formation and mRNA levels of osteogenic markers like BMP-2, Type 1 Collagen and RUNX-2. Based on these results, we propose that ethanolic extract of C. cristata and its pure compound coelogin have potential in the management of post menopausal osteoporosis.
authors.affiliation: Botany Division, CSIR-Central Drug Research Institute, Lucknow 226031, U.P., India.
authors.forename: Chetan
authors.initials: C
authors.lastname: Sharma
authors.affiliation: Endocrinology Division, CSIR-Central Drug Research Institute, Lucknow 226031, U.P., India.
authors.forename: Mohd Nizam
authors.initials: MN
authors.lastname: Mansoori
authors.affiliation: Endocrinology Division, CSIR-Central Drug Research Institute, Lucknow 226031, U.P., India.
authors.forename: Manisha
authors.initials: M
authors.lastname: Dixit
authors.affiliation: Endocrinology Division, CSIR-Central Drug Research Institute, Lucknow 226031, U.P., India.
authors.forename: Priyanka
authors.initials: P
authors.lastname: Shukla
authors.affiliation: Botany Division, CSIR-Central Drug Research Institute, Lucknow 226031, U.P., India.
authors.forename: Tejaswita
authors.initials: T
authors.lastname: Kumari
authors.affiliation: Medicinal and Process Chemistry Division, CSIR-Central Drug Research Institute, Lucknow 226031, U.P., India.
authors.forename: S P S
authors.initials: SP
authors.lastname: Bhandari
authors.affiliation: Medicinal and Process Chemistry Division, CSIR-Central Drug Research Institute, Lucknow 226031, U.P., India.
authors.forename: T
authors.initials: T
authors.lastname: Narender
authors.affiliation: Endocrinology Division, CSIR-Central Drug Research Institute, Lucknow 226031, U.P., India.
authors.forename: Divya
authors.initials: D
authors.lastname: Singh
authors.affiliation: Botany Division, CSIR-Central Drug Research Institute, Lucknow 226031, U.P., India. Electronic address: kr_arya@cdri.res.in.
authors.forename: K R
authors.initials: KR
authors.lastname: Arya
mesh_terms: D000818:Animals; D055396:Bone Morphogenetic Protein 2 / Q000378:metabolism; D002113:Calcification, Physiologic / Q000187:drug effects; D002478:Cells, Cultured; D024042:Collagen Type I / Q000378:metabolism; D050659:Core Binding Factor Alpha 1 Subunit / Q000378:metabolism; D004967:Estrogens / Q000172:deficiency; D000431:Ethanol; D005260:Female; D008807:Mice, Inbred BALB C; D029595:Orchidaceae / Q000737:chemistry*; D010006:Osteoblasts / Q000187:drug effects; D010012:Osteogenesis / Q000187:drug effects*; D010052:Ovariectomy; D010616:Phenanthrenes / Q000494:pharmacology*; D010936:Plant Extracts / Q000494:pharmacology*; D011714:Pyrans / Q000494:pharmacology*
chemical_list: C000596253:7,8-dimethoxy-9,10-dihydro-5H-naphtho(8,1,2-cde)chromene-2,6-diol; C525719:Bmp2 protein, mouse; D055396:Bone Morphogenetic Protein 2; D024042:Collagen Type I; D050659:Core Binding Factor Alpha 1 Subunit; D004967:Estrogens; D010616:Phenanthrenes; D010936:Plant Extracts; D011714:Pyrans; C493716:Runx2 protein, mouse; D000431:Ethanol
keywords: Bone healing; Coelogin; Coelogyne cristata; Orchid; Osteoporosis