title: Effects of sodium metabisulfite on the expression of BK(Ca), K(ATP), and L-Ca(2+) channels in rat aortas in vivo and in vitro.
abstract: Sodium metabisulfite (SMB) is most commonly used as the preservative in many food preparations and drugs. So far, few studies about its negative effects were reported. The purpose of this study was to investigate the effect of SMB on the expression of big-conductance Ca(2+)-activated K(+) (BKCa), ATP-sensitive K(+) (KATP), and L-type calcium (L-Ca(2+)) channels in rat aorta in vivo and in vitro. The results showed that the mRNA and protein levels of the BKCa channel subunits α and β1 of aorta in rats were increased by SMB in vivo and in vitro. Similarly, the expression of the KATP channel subunits Kir6.1, Kir6.2, and SUR2B were increased by SMB. However, SMB at the highest concentration significantly decreased the expression of the L-Ca(2+) channel subunits Cav1.2 and Cav1.3. These results suggest that SMB can activate BKCa and KATP channels by increasing the expression of α, β1, and Kir6.1, Kir6.2, SUR2B respectively, while also inhibit L-Ca(2+) channels by decreasing the expression of Cav1.2 and Cav1.3 of aorta in rats. The molecular mechanism of SMB-induced vasorelaxant effect might be related to the expression changes of BKCa, KATP, and L-Ca(2+) channels subunits. Further work is needed to determine the relative contribution of each channel in SMB-mediated vasorelaxant effect.
authors.affiliation: Institute of Environmental Medicine and Toxicology, Institute of Environmental Science, Shanxi University, Taiyuan 030006, China. Electronic address: qxzhang@sxu.edu.cn.
authors.forename: Quanxi
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: Institute of Environmental Medicine and Toxicology, Institute of Environmental Science, Shanxi University, Taiyuan 030006, China.
authors.forename: Yunlong
authors.initials: Y
authors.lastname: Bai
authors.affiliation: Institute of Environmental Medicine and Toxicology, Institute of Environmental Science, Shanxi University, Taiyuan 030006, China.
authors.forename: Jingjing
authors.initials: J
authors.lastname: Tian
authors.affiliation: Institute of Environmental Medicine and Toxicology, Institute of Environmental Science, Shanxi University, Taiyuan 030006, China.
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Lei
authors.affiliation: Institute of Environmental Medicine and Toxicology, Institute of Environmental Science, Shanxi University, Taiyuan 030006, China.
authors.forename: Mei
authors.initials: M
authors.lastname: Li
authors.affiliation: Institute of Environmental Medicine and Toxicology, Institute of Environmental Science, Shanxi University, Taiyuan 030006, China.
authors.forename: Zhenhua
authors.initials: Z
authors.lastname: Yang
authors.affiliation: Institute of Environmental Medicine and Toxicology, Institute of Environmental Science, Shanxi University, Taiyuan 030006, China.
authors.forename: Ziqiang
authors.initials: Z
authors.lastname: Meng
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D001011:Aorta / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology*; D016085:Bronchoconstrictor Agents / Q000737:chemistry; D015220:Calcium Channels / Q000378:metabolism; D020746:Calcium Channels, L-Type / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005786:Gene Expression Regulation / Q000187:drug effects; D054086:KATP Channels / Q000378:metabolism; D008297:Male; D009154:Mutation; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D012984:Software; D013447:Sulfites / Q000737:chemistry*; D014664:Vasodilation / Q000187:drug effects
chemical_list: D016085:Bronchoconstrictor Agents; D015220:Calcium Channels; D020746:Calcium Channels, L-Type; D054086:KATP Channels; D012333:RNA, Messenger; D013447:Sulfites; C493217:calcium channel, l type beta2c subunit, rat; C092520:uK-ATP-1 potassium channel; C093712:Cacna1d protein, rat; C005200:sodium metabisulfite; D000255:Adenosine Triphosphate
keywords: Aorta; BK(Ca) channels; K(ATP) channels; L-Ca(2+) channels; Sodium metabisulfite