title: Beneficial effect of nifedipine on testicular torsion-detorsion injury in rats.
abstract: OBJECTIVE
  To investigate the effect of nifedipine on testicular torsion-detorsion injury.
  
  
  METHODS
  Twenty-four adult male Sprague-Dawley rats were randomly divided into 3 groups, each containing 8 rats. Rats in the control group underwent a sham operation of the left testis. In the torsion-detorsion (T/D) group, the left testis was twisted at 720° for 3 hours. After 3 hours of reperfusion, at the end of the experiment, the testes were removed. Rats in the treatment group received the same surgical procedure as the T/D group, but nifedipine was administered intraperitoneally (100 μg/kg) 30 minutes before the time of detorsion.
  
  
  RESULTS
  Unilateral testicular torsion-detorsion caused a significant increase in the malondialdehyde level and apoptosis and caused significant decreases in superoxide dismutase and glutathione peroxidase activities in ipsilateral testes. The rats treated with nifedipine had a significant decrease in malondialdehyde level and apoptosis and had significant increases in superoxide dismutase and glutathione peroxidase activities in ipsilateral testes compared with those of the T/D group.
  
  
  CONCLUSIONS
  These results suggest that biochemical and histological torsion-detorsion injury occurs in the ipsilateral testes after a 3-hour torsion and 3-hour detorsion and that administration of nifedipine before detorsion prevents ischemia/reperfusion cellular damage in the testicular tissue.
authors.affiliation: Department of Pediatric Surgery, Split University Hospital Centre and Split University School of Medicine, Split, Croatia.
authors.forename: Jakov
authors.initials: J
authors.lastname: Meštrović
authors.affiliation: Department of Medical Chemistry and Biochemistry, University of Split School of Medicine, Split, Croatia; Department of Pathology, Forensic Medicine and Cytology, University of Split School of Medicine, Split, Croatia.
authors.forename: Irena
authors.initials: I
authors.lastname: Drmić-Hofman
authors.affiliation: Department of Pediatric Surgery, Split University Hospital Centre and Split University School of Medicine, Split, Croatia. Electronic address: zpogorelic@gmail.com.
authors.forename: Zenon
authors.initials: Z
authors.lastname: Pogorelić
authors.affiliation: Department of Pathology, Forensic Medicine and Cytology, Split University Hospital Centre and Split University School of Medicine, Split, Croatia.
authors.forename: Katarina
authors.initials: K
authors.lastname: Vilović
authors.affiliation: Department of Medical Laboratory Diagnosis, Split University Hospital Centre, Split, Croatia.
authors.forename: Daniela
authors.initials: D
authors.lastname: Šupe-Domić
authors.affiliation: Department of Pharmacology, University of Split School of Medicine, Split, Croatia.
authors.forename: Ana
authors.initials: A
authors.lastname: Šešelja-Perišin
authors.affiliation: Department of Nuclear Medicine, Split University Hospital Centre and Split University School of Medicine, Split, Croatia.
authors.forename: Vesna
authors.initials: V
authors.lastname: Capkun
mesh_terms: D000818:Animals; D017209:Apoptosis; D002121:Calcium Channel Blockers / Q000627:therapeutic use; D005979:Glutathione Peroxidase / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D009543:Nifedipine / Q000627:therapeutic use*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015424:Reperfusion; D013086:Spermatic Cord Torsion / Q000188:drug therapy*; D013482:Superoxide Dismutase / Q000378:metabolism; D013737:Testis / Q000187:drug effects*
chemical_list: D002121:Calcium Channel Blockers; D008315:Malondialdehyde; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D009543:Nifedipine