title: Oxytocinergic circuit from paraventricular and supraoptic nuclei to arcuate POMC neurons in hypothalamus.
abstract: Intracerebroventricular injection of oxytocin (Oxt), a neuropeptide produced in hypothalamic paraventricular (PVN) and supraoptic nuclei (SON), melanocortin-dependently suppresses feeding. However, the underlying neuronal pathway is unclear. This study aimed to determine whether Oxt regulates propiomelanocortin (POMC) neurons in the arcuate nucleus (ARC) of the hypothalamus. Intra-ARC injection of Oxt decreased food intake. Oxt increased cytosolic Ca(2+) in POMC neurons isolated from ARC. ARC POMC neurons expressed Oxt receptors and were contacted by Oxt terminals. Retrograde tracer study revealed the projection of PVN and SON Oxt neurons to ARC. These results demonstrate the novel oxytocinergic signaling from PVN/SON to ARC POMC, possibly regulating feeding.
authors.affiliation: Division of Integrative Physiology, Department of Physiology, Jichi Medical University School of Medicine, Shimotsuke, Tochigi 329-0498, Japan; Department of Electrophysiology and Oncology, Fukushima Medical University School of Medicine, Fukushima 960-1295, Japan.
authors.forename: Yuko
authors.initials: Y
authors.lastname: Maejima
authors.affiliation: Division of Integrative Physiology, Department of Physiology, Jichi Medical University School of Medicine, Shimotsuke, Tochigi 329-0498, Japan.
authors.forename: Kazuya
authors.initials: K
authors.lastname: Sakuma
authors.affiliation: Division of Integrative Physiology, Department of Physiology, Jichi Medical University School of Medicine, Shimotsuke, Tochigi 329-0498, Japan.
authors.forename: Putra
authors.initials: P
authors.lastname: Santoso
authors.affiliation: Division of Integrative Physiology, Department of Physiology, Jichi Medical University School of Medicine, Shimotsuke, Tochigi 329-0498, Japan.
authors.forename: Darambazar
authors.initials: D
authors.lastname: Gantulga
authors.affiliation: Division of Integrative Physiology, Department of Physiology, Jichi Medical University School of Medicine, Shimotsuke, Tochigi 329-0498, Japan.
authors.forename: Kenichi
authors.initials: K
authors.lastname: Katsurada
authors.affiliation: Department of Physiology, School of Medicine, University of Occupational and Environmental Health, Kitakyushu 807-8555, Japan.
authors.forename: Yoichi
authors.initials: Y
authors.lastname: Ueta
authors.affiliation: Department of Molecular and Cell Biology, Graduate School of Agricultural Science, Tohoku University, Miyagi 981-8555, Japan.
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Hiraoka
authors.affiliation: Department of Molecular and Cell Biology, Graduate School of Agricultural Science, Tohoku University, Miyagi 981-8555, Japan.
authors.forename: Katsuhiko
authors.initials: K
authors.lastname: Nishimori
authors.affiliation: Department of Biology, Faculty of Science, Shizuoka University, Ohya 836, Shizuoka 422-8529, Japan.
authors.forename: Shigeyasu
authors.initials: S
authors.lastname: Tanaka
authors.affiliation: Division of Integrative Physiology, Department of Physiology, Jichi Medical University School of Medicine, Shimotsuke, Tochigi 329-0498, Japan; Department of Electrophysiology and Oncology, Fukushima Medical University School of Medicine, Fukushima 960-1295, Japan.
authors.forename: Kenju
authors.initials: K
authors.lastname: Shimomura
authors.affiliation: Division of Integrative Physiology, Department of Physiology, Jichi Medical University School of Medicine, Shimotsuke, Tochigi 329-0498, Japan; Department of Developmental Physiology, Division of Adaptation Development, National Institute for Physiological Sciences, Okazaki, Aichi 444-8585, Japan. Electronic address: tyada@jichi.ac.jp.
authors.forename: Toshihiko
authors.initials: T
authors.lastname: Yada
mesh_terms: D000818:Animals; D001111:Arcuate Nucleus of Hypothalamus / Q000166:cytology* / Q000187:drug effects; D002552:Cerebral Ventricles / Q000166:cytology* / Q000187:drug effects; D005786:Gene Expression Regulation / Q000187:drug effects; D008297:Male; D009474:Neurons / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism*; D010121:Oxytocin / Q000494:pharmacology*; D011333:Pro-Opiomelanocortin / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D018045:Receptors, Oxytocin / Q000378:metabolism; D013495:Supraoptic Nucleus / Q000166:cytology* / Q000187:drug effects
chemical_list: D018045:Receptors, Oxytocin; D010121:Oxytocin; D011333:Pro-Opiomelanocortin
keywords: Arcuate nucleus; Feeding; Obesity; Oxytocin; Proopiomelanocortin