title: Development and validation of a rapid resolution liquid chromatography method for the screening of dietary plant isoprenoids: carotenoids, tocopherols and chlorophylls.
abstract: A rapid resolution liquid chromatography (RRLC) method was developed and validated for the simultaneous determination of nine carotenoids compounds (violaxanthin, lutein, zeaxanthin, β-cryptoxanthin, α-carotene, β-carotene, lycopene, phytoene, phytofluene), four tocopherols and four chlorophylls and derivates (chlorophylls and pheophytins). The methodology consisted in a micro-extraction procedure with or without saponification and subsequent analysis by RRLC. The limits of detection were <0.07 μg for carotenoids and tocopherols and <0.08 μg for chlorophylls and derivatives. The overall precision values (intra- and inter-day) were lower than 12% when samples were not saponified and <27.6%, when the saponification step was performed. The recovery of the method without the saponification step ranged from 92% to 107%, whilst that when saponification was carried out ranged from 60% for α-tocopherol to 82% for β-carotene. Finally, the applicability of the method was demonstrated by the identification and quantification of isoprenoids in different samples. The methodology is appropriate for the high-throughput screening of dietary isoprenoids in fruits and vegetables.
authors.affiliation: Food Colour & Quality Laboratory, Department of Nutrition & Food Science, Universidad de Sevilla, Facultad de Farmacia, 41012 Sevilla, Spain.
authors.forename: Carla M
authors.initials: CM
authors.lastname: Stinco
authors.affiliation: Food Colour & Quality Laboratory, Department of Nutrition & Food Science, Universidad de Sevilla, Facultad de Farmacia, 41012 Sevilla, Spain.
authors.forename: Ana M
authors.initials: AM
authors.lastname: Benítez-González
authors.affiliation: Department of Analytical Chemistry, Universidad de Sevilla, Facultad de Farmacia, 41012 Sevilla, Spain.
authors.forename: Dolores
authors.initials: D
authors.lastname: Hernanz
authors.affiliation: Food Colour & Quality Laboratory, Department of Nutrition & Food Science, Universidad de Sevilla, Facultad de Farmacia, 41012 Sevilla, Spain.
authors.forename: Isabel M
authors.initials: IM
authors.lastname: Vicario
authors.affiliation: Food Colour & Quality Laboratory, Department of Nutrition & Food Science, Universidad de Sevilla, Facultad de Farmacia, 41012 Sevilla, Spain. Electronic address: ajmelendez@us.es.
authors.forename: Antonio J
authors.initials: AJ
authors.lastname: Meléndez-Martínez
mesh_terms: D002734:Chlorophyll / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005638:Fruit / Q000737:chemistry; D015203:Reproducibility of Results; D013729:Terpenes / Q000032:analysis; D013997:Time Factors; D024505:Tocopherols / Q000032:analysis; D014675:Vegetables / Q000737:chemistry
chemical_list: D013729:Terpenes; D002734:Chlorophyll; D024505:Tocopherols
keywords: Carotenoids; Chlorophylls; Isoprenoids; Phytoene; Phytofluene; Tocopherols