title: Positive effect of IGF-1 injection on gastrocnemius of rat during distraction osteogenesis.
abstract: Distraction osteogenesis (DO) is used to form new bone between bone segments to lengthen the callus. Skeletal muscles frequently fail to adapt to distraction, which causes complications. Insulin-like growth factor-1 (IGF-1) has been implicated as a central regulator of muscle repair. We hypothesized that IGF-1 injection could reduce muscle complications in DO. A total of 102 Sprague-Dawley rats received DO or did not were randomly assigned into saline, IGF-1 and normal groups. On the day before the distraction, the rats in the IGF-1 group were injected with IGF-1. The gastrocnemius muscles of the rats were harvested at the 0, 1st, 4th, 7th, and 10th days of distraction. The weight of the muscles, cross-sectional area (CSA) of the muscle fibers, collagen volume fraction (CVF), maximum limit load (MLL), maximum contraction forces, and gene expression of Akt, MyoD, myogenin, myostatin, and collagen I were analyzed. The results indicated that IGF-1 injection had increased the weights, CSA of the muscle fibers, MLL and force generation of the gastrocnemius. Also, Akt, MyoD, and myogenin were upregulated, and myostatin was downregulated in the IGF-1 group. Injection of IGF-1 could attenuate the gastrocnemius atrophy, prevent fibrosis, increase MLL, and regulate the related mRNA.
authors.affiliation: Biomechanics Labs of Orthopaedics Research Institute, Tianjin Hospital Heping Branch, 122 Munan Street, Heping District, Tianjin, 300050, China.|Tianjin Medical University, No. 22 Qixiangtai Street, Heping District, Tianjin, 300052, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Feng
authors.affiliation: Biomechanics Labs of Orthopaedics Research Institute, Tianjin Hospital Heping Branch, 122 Munan Street, Heping District, Tianjin, 300050, China.
authors.forename: Xinlong
authors.initials: X
authors.lastname: Ma
authors.affiliation: Biomechanics Labs of Orthopaedics Research Institute, Tianjin Hospital Heping Branch, 122 Munan Street, Heping District, Tianjin, 300050, China.
authors.forename: Jianxiong
authors.initials: J
authors.lastname: Ma
authors.affiliation: Biomechanics Labs of Orthopaedics Research Institute, Tianjin Hospital Heping Branch, 122 Munan Street, Heping District, Tianjin, 300050, China.|Tianjin Medical University, No. 22 Qixiangtai Street, Heping District, Tianjin, 300052, China.
authors.forename: Haobo
authors.initials: H
authors.lastname: Jia
authors.affiliation: Biomechanics Labs of Orthopaedics Research Institute, Tianjin Hospital Heping Branch, 122 Munan Street, Heping District, Tianjin, 300050, China.
authors.forename: Baoyi
authors.initials: B
authors.lastname: Ma
authors.affiliation: Biomechanics Labs of Orthopaedics Research Institute, Tianjin Hospital Heping Branch, 122 Munan Street, Heping District, Tianjin, 300050, China.|Tianjin Medical University, No. 22 Qixiangtai Street, Heping District, Tianjin, 300052, China.
authors.forename: Liyan
authors.initials: L
authors.lastname: Xu
authors.affiliation: First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, No. 314, Anshan Road, Nankai District, Tianjin, 300193, China.
authors.forename: Aifeng
authors.initials: A
authors.lastname: Liu
mesh_terms: D000818:Animals; D024042:Collagen Type I / Q000378:metabolism; D004353:Drug Evaluation, Preclinical; D007334:Insulin-Like Growth Factor I / Q000494:pharmacology / Q000627:therapeutic use*; D008297:Male; D018482:Muscle, Skeletal / Q000187:drug effects* / Q000378:metabolism; D009133:Muscular Atrophy / Q000209:etiology / Q000517:prevention & control*; D019857:Osteogenesis, Distraction / Q000009:adverse effects*; D011897:Random Allocation; D017207:Rats, Sprague-Dawley; D012038:Regeneration / Q000187:drug effects*
chemical_list: D024042:Collagen Type I; D007334:Insulin-Like Growth Factor I
keywords: Distraction osteogenesis (DO); Gastrocnemius; Injection; Insulin-like growth factor 1(IGF-1)