title: [Effects of artificial shrinkage prior to vitrification in a closed system: a randomized controlled trial].
abstract: OBJECTIVE
  To evaluate the effect of induced blastocoele shrinkage before vitrification in a closed carrier device.
  
  
  METHODS
  Prior to vitrification, blastocyst cavity was artificially shrinked by laser pulse or not treated according to a 2:1 randomized procedure. A total of 185 warming cycles from April 2011 to March 2013 have been analyzed. Clinical pregnancy rate and survival rate were compared between the two groups. The mean (±SD) women age was 33.5±5.7 years for both groups.
  
  
  RESULTS
  The pregnancy rate in the group with artificial reduction of the cavity was higher ([32/67] 47.7%) than in the control group but not significantly ([43/113] 38%). The survival rate in the artificial shrinkage group was significantly higher compared with the control group : 99% (102/103) and 91.8% (168/183) respectively (P=0.01).
  
  
  CONCLUSIONS
  This study reveals that artificial shrinkage of blastocoelic cavity by laser pulse before vitrification in a closed carrier device improves survival rate after warming.
authors.affiliation: Institut de recherche en biothérapie, hôpital Saint-Éloi, CHU de Montpellier, 34000 Montpellier, France; U1040, Inserm, 34000 Montpellier, France; UFR de médecine, université Montpellier-1, 34000 Montpellier, France; Service de gynécologie-obstétrique, hôpital Arnaud-de-Villeneuve, CHU de Montpellier, 34295 Montpellier cedex 5, France.
authors.forename: A
authors.initials: A
authors.lastname: Gala
authors.affiliation: Institut de recherche en biothérapie, hôpital Saint-Éloi, CHU de Montpellier, 34000 Montpellier, France; U1040, Inserm, 34000 Montpellier, France; Service de gynécologie-obstétrique, hôpital Arnaud-de-Villeneuve, CHU de Montpellier, 34295 Montpellier cedex 5, France.
authors.forename: A
authors.initials: A
authors.lastname: Ferrières
authors.affiliation: Institut de recherche en biothérapie, hôpital Saint-Éloi, CHU de Montpellier, 34000 Montpellier, France; U1040, Inserm, 34000 Montpellier, France; UFR de médecine, université Montpellier-1, 34000 Montpellier, France.
authors.forename: S
authors.initials: S
authors.lastname: Assou
authors.affiliation: Service de gynécologie-obstétrique, hôpital Arnaud-de-Villeneuve, CHU de Montpellier, 34295 Montpellier cedex 5, France.
authors.forename: M
authors.initials: M
authors.lastname: Monforte
authors.affiliation: Service de gynécologie-obstétrique, hôpital Arnaud-de-Villeneuve, CHU de Montpellier, 34295 Montpellier cedex 5, France.
authors.forename: S
authors.initials: S
authors.lastname: Bringer-Deutsch
authors.affiliation: Service de gynécologie-obstétrique, hôpital Arnaud-de-Villeneuve, CHU de Montpellier, 34295 Montpellier cedex 5, France.
authors.forename: E
authors.initials: E
authors.lastname: Vintejoux
authors.affiliation: Service de gynécologie-obstétrique, hôpital Arnaud-de-Villeneuve, CHU de Montpellier, 34295 Montpellier cedex 5, France.
authors.forename: C
authors.initials: C
authors.lastname: Vincens
authors.affiliation: Service de gynécologie-obstétrique, hôpital Arnaud-de-Villeneuve, CHU de Montpellier, 34295 Montpellier cedex 5, France.
authors.forename: C
authors.initials: C
authors.lastname: Brunet
authors.affiliation: Institut de recherche en biothérapie, hôpital Saint-Éloi, CHU de Montpellier, 34000 Montpellier, France; U1040, Inserm, 34000 Montpellier, France; UFR de médecine, université Montpellier-1, 34000 Montpellier, France; Service de gynécologie-obstétrique, hôpital Arnaud-de-Villeneuve, CHU de Montpellier, 34295 Montpellier cedex 5, France. Electronic address: s-hamamah@chu-montpellier.fr.
authors.forename: S
authors.initials: S
authors.lastname: Hamamah
mesh_terms: D000328:Adult; D001755:Blastocyst / Q000502:physiology* / Q000648:ultrastructure*; D015925:Cryopreservation / Q000295:instrumentation / Q000379:methods*; D004624:Embryo Transfer; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods; D006358:Hot Temperature; D006801:Humans; D011247:Pregnancy; D018873:Pregnancy Rate
keywords: Artificial shrinkage; Blastocoele; Blastocyst; Blastocystes; Blastocèle; Closed system; Collapsing; Réduction artificielle cavité; Système fermé; Vitrification