title: Kinetics of mesenchymal and hematopoietic stem cells mobilization by G-CSF and its impact on the cytokine microenvironment in primary cultures.
abstract: In this study, we demonstrate that G-CSF administration triggers distinct kinetics of stem cell-SC mobilization with early raise of hematopoietic-HSC and late increase of mesenchymal-MSC in bone marrow-BM and peripheral blood-PB. The cytokine microenvironment observed following primary cultures showed an overall G-CSF dose-dependent profile with a clear mixed pro-inflammatory/regulatory pattern. Moreover, primary cultures performed at the peak of MSC/HSC ratio, showed distinct cytokine patterns, with higher IL-10, TNF-α and IL-17A observed for BM and enhanced IL-10, IL-2 and IFN-γ for PB harvested cells. Positive correlation was observed between BM-MSC and the levels of TNF-α, IL-10 and IL-17A whereas negative correlation was found between IL-10 and BM-HSC. An opposite association was observed between IL-10 and PB-HSC. Our results support the hypothesis that MSC and HSC harvested from BM and PB display differential functional properties that should be considered when electing the SC sources available for cell therapy applied in clinical protocols.
authors.affiliation: Post-Graduation Program in Health Science, Federal University of Amazonas - UFAM, CEP 69077-000 Manaus, AM, Brazil; Department of Research, Hematology and Hemotherapy Foundation of Amazonas - HEMOAM, CEP 69050-001 Manaus, AM, Brazil.
authors.forename: Nadja Pinto
authors.initials: NP
authors.lastname: Garcia
authors.affiliation: Multi-Institutional Program of Post-Graduation in Biotechnology, Federal University of Amazonas - UFAM, CEP 69077-000 Manaus, AM, Brazil.
authors.forename: Elisa Brosina
authors.initials: EB
authors.lastname: de Leon
authors.affiliation: Department of Research, Hematology and Hemotherapy Foundation of Amazonas - HEMOAM, CEP 69050-001 Manaus, AM, Brazil; Post-Graduation Program in Basic and Applied Immunology, Federal University of Amazonas - UFAM, CEP 69077-000 Manaus, AM, Brazil.
authors.forename: Allyson Guimarães
authors.initials: AG
authors.lastname: da Costa
authors.affiliation: Department of Research, Hematology and Hemotherapy Foundation of Amazonas - HEMOAM, CEP 69050-001 Manaus, AM, Brazil; Post-Graduation Program in Basic and Applied Immunology, Federal University of Amazonas - UFAM, CEP 69077-000 Manaus, AM, Brazil.
authors.forename: Andréa Monteiro
authors.initials: AM
authors.lastname: Tarragô
authors.affiliation: Department of Research, Hematology and Hemotherapy Foundation of Amazonas - HEMOAM, CEP 69050-001 Manaus, AM, Brazil; Leônidas and Maria Deane Research Center, Oswaldo Cruz Foundation - FIOCRUZ, CEP 69057-070 Manaus, AM, Brazil.
authors.forename: João Paulo
authors.initials: JP
authors.lastname: Pimentel
authors.affiliation: Post-Graduation Program in Basic and Applied Immunology, Federal University of Amazonas - UFAM, CEP 69077-000 Manaus, AM, Brazil.
authors.forename: Liziara
authors.initials: L
authors.lastname: Fraporti
authors.affiliation: René Rachou Research Center, Laboratory of Biomarkers for Diagnosis and Monitoring, Oswaldo Cruz Foundation - FIOCRUZ, CEP 30190-002 Belo Horizonte, MG, Brazil.
authors.forename: Fernanda Fortes
authors.initials: FF
authors.lastname: de Araujo
authors.affiliation: René Rachou Research Center, Laboratory of Biomarkers for Diagnosis and Monitoring, Oswaldo Cruz Foundation - FIOCRUZ, CEP 30190-002 Belo Horizonte, MG, Brazil.
authors.forename: Fernanda Magalhães Freire
authors.initials: FM
authors.lastname: Campos
authors.affiliation: René Rachou Research Center, Laboratory of Biomarkers for Diagnosis and Monitoring, Oswaldo Cruz Foundation - FIOCRUZ, CEP 30190-002 Belo Horizonte, MG, Brazil.
authors.forename: Andréa
authors.initials: A
authors.lastname: Teixeira-Carvalho
authors.affiliation: René Rachou Research Center, Laboratory of Biomarkers for Diagnosis and Monitoring, Oswaldo Cruz Foundation - FIOCRUZ, CEP 30190-002 Belo Horizonte, MG, Brazil. Electronic address: oamfilho@cpqrr.fiocruz.br.
authors.forename: Olindo Assis
authors.initials: OA
authors.lastname: Martins-Filho
authors.affiliation: Post-Graduation Program in Health Science, Federal University of Amazonas - UFAM, CEP 69077-000 Manaus, AM, Brazil; Department of Research, Hematology and Hemotherapy Foundation of Amazonas - HEMOAM, CEP 69050-001 Manaus, AM, Brazil; Post-Graduation Program in Basic and Applied Immunology, Federal University of Amazonas - UFAM, CEP 69077-000 Manaus, AM, Brazil.
authors.forename: Adriana
authors.initials: A
authors.lastname: Malheiro
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology / Q000187:drug effects* / Q000276:immunology; D004306:Dose-Response Relationship, Immunologic; D005260:Female; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology*; D019650:Hematopoietic Stem Cell Mobilization; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000187:drug effects* / Q000276:immunology; D016130:Immunophenotyping; D016753:Interleukin-10 / Q000096:biosynthesis / Q000378:metabolism; D020381:Interleukin-17 / Q000096:biosynthesis / Q000378:metabolism; D007376:Interleukin-2 / Q000096:biosynthesis / Q000378:metabolism; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000187:drug effects* / Q000276:immunology; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects* / Q000276:immunology; D051379:Mice; D061251:Primary Cell Culture; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis / Q000378:metabolism
chemical_list: C556062:IL10 protein, mouse; D020381:Interleukin-17; D007376:Interleukin-2; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10; D016179:Granulocyte Colony-Stimulating Factor
keywords: Cytokines; Hematopoietic; Mesenchymal; Stem cells mobilization