title: Identification of whole pathogenic cells by monoclonal antibodies generated against a specific peptide from an immunogenic cell wall protein.
abstract: We selected the immunogenic cell wall ß-(1,3)-glucosyltransferase Bgl2p from Candida albicans as a target protein for the production of antibodies. We identified a unique peptide sequence in the protein and generated monoclonal anti- C. albicans Bgl2p antibodies, which bound in particular to whole C. albicans cells.
authors.affiliation: Helmholtz Centre for Infection Research (HZI), Inhoffenstr. 7, 38124 Braunschweig, Germany.
authors.forename: Hani E J
authors.initials: HE
authors.lastname: Kaba
authors.affiliation: Institute of Biochemistry and Biology, University of Potsdam, Karl-Liebknecht-Str. 24-25, 14476 Golm, Germany.
authors.forename: Natalia
authors.initials: N
authors.lastname: Maier
authors.affiliation: Institute of Biochemistry and Biology, University of Potsdam, Karl-Liebknecht-Str. 24-25, 14476 Golm, Germany.
authors.forename: Nicole
authors.initials: N
authors.lastname: Schliebe-Ohler
authors.affiliation: MicroDiscovery GmbH, Marienburger Str., 1, 10405 Berlin, Germany.
authors.forename: Yvonne
authors.initials: Y
authors.lastname: Mayer
authors.affiliation: Helmholtz Centre for Infection Research (HZI), Inhoffenstr. 7, 38124 Braunschweig, Germany.
authors.forename: Peter P
authors.initials: PP
authors.lastname: Müller
authors.affiliation: Helmholtz Centre for Infection Research (HZI), Inhoffenstr. 7, 38124 Braunschweig, Germany.
authors.forename: Joop
authors.initials: J
authors.lastname: van den Heuvel
authors.affiliation: MicroDiscovery GmbH, Marienburger Str., 1, 10405 Berlin, Germany.
authors.forename: Johannes
authors.initials: J
authors.lastname: Schuchhardt
authors.affiliation: Institute of Biochemistry and Biology, University of Potsdam, Karl-Liebknecht-Str. 24-25, 14476 Golm, Germany.
authors.forename: Katja
authors.initials: K
authors.lastname: Hanack
authors.affiliation: Helmholtz Centre for Infection Research (HZI), Inhoffenstr. 7, 38124 Braunschweig, Germany. Electronic address: Ursula.Bilitewski@helmholtz-hzi.de.
authors.forename: Ursula
authors.initials: U
authors.lastname: Bilitewski
mesh_terms: D000911:Antibodies, Monoclonal / Q000032:analysis* / Q000276:immunology; D002176:Candida albicans / Q000166:cytology / Q000201:enzymology / Q000276:immunology / Q000302:isolation & purification*; D002177:Candidiasis / Q000382:microbiology; D002473:Cell Wall / Q000737:chemistry / Q000201:enzymology / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005656:Fungal Proteins / Q000032:analysis* / Q000276:immunology; D005964:Glucosyltransferases / Q000032:analysis / Q000276:immunology; D006801:Humans; D010455:Peptides / Q000032:analysis* / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D005656:Fungal Proteins; D010455:Peptides; D005964:Glucosyltransferases
keywords: Bgl2p; Candida; Diagnostics; Flow cytometry; Peptides