title: Effects and action mechanisms of sodium fluoride (NaF) on the growth and cephalotaxine production of Cephalotaxus mannii suspension cells.
abstract: To explore the effects of NaF on the growth and cephalotaxine production of Cephalotaxus mannii suspension cells, NaF was added into the C mannii cell suspension cultures at day 15 of culture time. It is documented that NaF suppressed cell growth but enhanced cephalotaxine production. The largest yield obtained of cephalotaxine reached to 9.57mg/L when the cultures were treated with an appropriate dosage of 15mg/L NaF, which was 3.7 times that of the control cultures (2.58mg/L). Additionally, NaF markedly enhanced the activity of glucose 6-phosphate dehydrogenase (G6PDH) and reduced the level of hydrogen peroxide (H2O2) of cells. It was also found that NaF weakened the oxidative damage of cell membrane and led to lower content of malonyl dialdehyde (MDA) in NaF-treated cells compared with the control cells. The MDA content of NaF-treated cells decreased 91% compared to the controls. Although lower membrane lipid peroxidation in NaF-treated cells, its membrane permeability was higher than the control cells and showed a high product secret rate. What is more, NaF boosted the activity of phenylalanine ammonium-lyse (PAL), but did not burst a peak of PAL activity in the time curve of PAL activity. These results indicated NaF acted as an inhibitor of the Enbden Parnas (EMP) pathway, not as an elicitor to promote cephalotaxine production.
authors.affiliation: College of Food, Hainan University, Haikou 570228, PR China. Electronic address: lyc2360@163.com.
authors.forename: Yong-Cheng
authors.initials: YC
authors.lastname: Li
authors.affiliation: College of Food, Hainan University, Haikou 570228, PR China.
authors.forename: Xuan-Xian
authors.initials: XX
authors.lastname: Jiang
authors.affiliation: College of Food, Hainan University, Haikou 570228, PR China.
authors.forename: Xiao-Juan
authors.initials: XJ
authors.lastname: Long
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000096:biosynthesis*; D002463:Cell Membrane Permeability / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D032483:Cephalotaxus / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism*; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism; D006248:Harringtonines / Q000096:biosynthesis*; D000077863:Homoharringtonine; D015227:Lipid Peroxidation / Q000187:drug effects; D008315:Malondialdehyde / Q000378:metabolism; D053858:Metabolic Networks and Pathways / Q000187:drug effects; D010650:Phenylalanine Ammonia-Lyase / Q000378:metabolism; D012969:Sodium Fluoride / Q000494:pharmacology*
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D006248:Harringtonines; D008315:Malondialdehyde; D000077863:Homoharringtonine; D012969:Sodium Fluoride; D005954:Glucosephosphate Dehydrogenase; D010650:Phenylalanine Ammonia-Lyase
keywords: Cephalotaxus mannii; Glucose 6-phosphate dehydrogenase; Metabolic inhibitor; Sodium fluoride; Suspension culture