title: Anti-HIV activity of fucoidans from three brown seaweed species.
abstract: Fucoidans are sulfated polysaccharides derived from marine brown algae. In the current work the anti-HIV activity of three fucoidans, extracted from three brown seaweeds Sargassum mcclurei, Sargassum polycystum and Turbinara ornata and collected from Nha Trang bay, Vietnam was investigated. Fucoidans extracted from the three species displayed similar antiviral activities with a mean IC50 ranging from 0.33 to 0.7 μg/ml while displaying no cell toxicity. Our results showed that the anti-HIV activity of fucoidans is not primarily linked to the sulfate content and the appropriate position of sulfate groups in the fucoidan backbones was also not associated with the antiviral activity. Fucoidans inhibited HIV-1 infection when they were pre-incubated with the virus but not with the cells, and not after infection, blocking the early steps of HIV entry into target cells. These data contribute to a better understanding of the influence of fucoidans structural characteristics on their biological activity.
authors.affiliation: Institute of Chemistry, Vietnam Academy of Science and Technology, Viet Nam. Electronic address: thuyttt@ich.vast.ac.vn.
authors.forename: Thanh Thi Thu
authors.initials: TT
authors.lastname: Thuy
authors.affiliation: Nha Trang Institute of Technology Research and Application, Vietnam Academy of Science and Technology, Viet Nam.
authors.forename: Bui Minh
authors.initials: BM
authors.lastname: Ly
authors.affiliation: Nha Trang Institute of Technology Research and Application, Vietnam Academy of Science and Technology, Viet Nam.
authors.forename: Tran Thi Thanh
authors.initials: TT
authors.lastname: Van
authors.affiliation: Institute of Chemistry, Vietnam Academy of Science and Technology, Viet Nam.
authors.forename: Ngo Van
authors.initials: NV
authors.lastname: Quang
authors.affiliation: Laboratory of Retrovirology, CRP-Santé, Luxembourg.
authors.forename: Ho Cam
authors.initials: HC
authors.lastname: Tu
authors.affiliation: Laboratory of Retrovirology, CRP-Santé, Luxembourg.
authors.forename: Yue
authors.initials: Y
authors.lastname: Zheng
authors.affiliation: Laboratory of Retrovirology, CRP-Santé, Luxembourg.
authors.forename: Carole
authors.initials: C
authors.lastname: Seguin-Devaux
authors.affiliation: N.D.Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninskii Prospect 47, Moscow 119991, Russian Federation.
authors.forename: Bilan
authors.initials: B
authors.lastname: Mi
authors.affiliation: N.D.Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninskii Prospect 47, Moscow 119991, Russian Federation.
authors.forename: Usov
authors.initials: U
authors.lastname: Ai
mesh_terms: D019380:Anti-HIV Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D002236:Carbohydrate Conformation; D045744:Cell Line, Tumor; D002470:Cell Survival; D004305:Dose-Response Relationship, Drug; D015658:HIV Infections / Q000188:drug therapy / Q000821:virology; D015497:HIV-1 / Q000187:drug effects*; D006801:Humans; D008826:Microbial Sensitivity Tests; D000459:Phaeophyceae / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D012624:Seaweed / Q000737:chemistry*; D013045:Species Specificity; D013329:Structure-Activity Relationship
chemical_list: D019380:Anti-HIV Agents; D011134:Polysaccharides; C007789:fucoidan
keywords: Anti-HIV activity; Brown seaweed; Fucoidan; Sargassum mcclurei; Sargassum polycystum; Turbinara ornata