title: NMR-based molecular ruler for determining the depth of intercalants within the lipid bilayer. Part V: a comparison of liposomes, bioliposomes and erythrocyte ghosts.
abstract: Afri et al. (2014a,b) have recently reported their mapping of DMPC liposomes using (13)C NMR in conjunction with a wide range of difunctional intercalants: n-ketoesters, n-ketoacids and n-ketophosphatidylcholines. The present study initiates a comparable study of bioliposomes and erythrocyte ghosts. This required the (13)C NMR characterization of these systems for the first time, and further involved a determination of the signals of three doubly (13)C-labeled intercalants, in particular, n-ketophosphatidylcholines where n=4, 8 and 12. This study reveals that DMPC liposomes, bioliposomes and erythrocyte ghosts, with all their structural differences, are not radically different from the perspective of polarity gradient. Any differences observed reflect the additives often naturally present in these lipid systems.
authors.affiliation: The Department of Chemistry, Bar-Ilan University, Ramat Gan 5290002, Israel. Electronic address: Michal.Afri@biu.ac.il.
authors.forename: Michal
authors.initials: M
authors.lastname: Afri
authors.affiliation: The Department of Chemistry, Bar-Ilan University, Ramat Gan 5290002, Israel. Electronic address: C_Eliasi@walla.com.
authors.forename: Carmit
authors.initials: C
authors.lastname: Alexenberg
authors.affiliation: The Department of Chemistry, Bar-Ilan University, Ramat Gan 5290002, Israel. Electronic address: Efrat.Bodner@biu.ac.il.
authors.forename: Efrat
authors.initials: E
authors.lastname: Bodner
authors.affiliation: The Department of Chemistry, Bar-Ilan University, Ramat Gan 5290002, Israel. Electronic address: Aryeh.Frimer@biu.ac.il.
authors.forename: Aryeh A
authors.initials: AA
authors.lastname: Frimer
authors.affiliation: Faculty of Life Sciences, Bar-Ilan University, Ramat Gan 5290002, Israel. Electronic address: Avi.Jacob@biu.ac.il.
authors.forename: Abraham I
authors.initials: AI
authors.lastname: Jacob
authors.affiliation: The Department of Chemistry, Bar-Ilan University, Ramat Gan 5290002, Israel. Electronic address: MiriamNaqqash@gmail.com.
authors.forename: Miriam E
authors.initials: ME
authors.lastname: Naqqash
mesh_terms: D003374:Coumarins / Q000737:chemistry; D004134:Dimyristoylphosphatidylcholine / Q000737:chemistry; D004910:Erythrocyte Membrane / Q000737:chemistry*; D007364:Intercalating Agents / Q000737:chemistry; D008051:Lipid Bilayers / Q000737:chemistry*; D008081:Liposomes / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008856:Microscopy, Fluorescence; D013816:Thermodynamics
chemical_list: D003374:Coumarins; D007364:Intercalating Agents; D008051:Lipid Bilayers; D008081:Liposomes; D004134:Dimyristoylphosphatidylcholine
keywords: (13)C NMR; Bioliposome; DMPC liposome; E(T)(30); Erythrocyte ghost; Ketophospholipids