title: Miniature direct electron transfer based sulphite/oxygen enzymatic fuel cells.
abstract: A direct electron transfer (DET) based sulphite/oxygen biofuel cell is reported that utilises human sulphite oxidase (hSOx) and Myrothecium verrucaria bilirubin oxidase (MvBOx) and nanostructured gold electrodes. For bioanode construction, the nanostructured gold microelectrodes were further modified with 3,3'-dithiodipropionic acid di(N-hydroxysuccinimide ester) to which polyethylene imine was covalently attached. hSOx was adsorbed onto this chemically modified nanostructured electrode with high surface loading of electroactive enzyme and in presence of sulphite high anodic bioelectrocatalytic currents were generated with an onset potential of 0.05V vs. NHE. The biocathode contained MvBOx directly adsorbed to the deposited gold nanoparticles for cathodic oxygen reduction starting at 0.71V vs. NHE. Both enzyme electrodes were integrated to a DET-type biofuel cell. Power densities of 8 and 1μWcm(-2) were achieved at 0.15V and 0.45V of cell voltages, respectively, with the membrane based biodevices under aerobic conditions.
authors.affiliation: Institut für Biochemie und Biologie, Universität Potsdam, Karl-Liebknecht-Str.24-25, 14476 Potsdam/Golm, Germany.
authors.forename: T
authors.initials: T
authors.lastname: Zeng
authors.affiliation: Biomedical Science, Health & Society, Malmö University, 205 06 Malmö, Sweden; Kurchatov NBIC Centre, National Research Centre "Kurchatov Institute", 123182 Moscow, Russia.
authors.forename: D
authors.initials: D
authors.lastname: Pankratov
authors.affiliation: Biomedical Science, Health & Society, Malmö University, 205 06 Malmö, Sweden.
authors.forename: M
authors.initials: M
authors.lastname: Falk
authors.affiliation: Institut für Biochemie und Biologie, Universität Potsdam, Karl-Liebknecht-Str.24-25, 14476 Potsdam/Golm, Germany.
authors.forename: S
authors.initials: S
authors.lastname: Leimkühler
authors.affiliation: Biomedical Science, Health & Society, Malmö University, 205 06 Malmö, Sweden; Kurchatov NBIC Centre, National Research Centre "Kurchatov Institute", 123182 Moscow, Russia. Electronic address: sergey.shleev@mah.se.
authors.forename: S
authors.initials: S
authors.lastname: Shleev
authors.affiliation: Institut für Biochemie und Biologie, Universität Potsdam, Karl-Liebknecht-Str.24-25, 14476 Potsdam/Golm, Germany. Electronic address: uwollen@uni-potsdam.de.
authors.forename: U
authors.initials: U
authors.lastname: Wollenberger
mesh_terms: D001674:Bioelectric Energy Sources; D015374:Biosensing Techniques / Q000295:instrumentation*; D004579:Electron Transport; D004583:Electrons; D004800:Enzymes, Immobilized / Q000378:metabolism; D004867:Equipment Design; D006801:Humans; D006999:Hypocreales / Q000201:enzymology*; D044925:Oxidoreductases Acting on CH-CH Group Donors / Q000378:metabolism*; D010100:Oxygen / Q000378:metabolism*; D050876:Sulfite Oxidase / Q000378:metabolism*; D013447:Sulfites / Q000378:metabolism
chemical_list: D004800:Enzymes, Immobilized; D013447:Sulfites; D044925:Oxidoreductases Acting on CH-CH Group Donors; C042401:bilirubin oxidase; D050876:Sulfite Oxidase; D010100:Oxygen
keywords: Bilirubin oxidase; Direct electron transfer; Enzymatic fuel cell; Microscale electrode; Sulphite oxidase