title: Pentavalent outer membrane vesicles of Vibrio cholerae induce adaptive immune response and protective efficacy in both adult and passive suckling mice models.
abstract: Recently, we demonstrated oral immunizations with single serotype outer membrane vesicles of Vibrio cholerae induced serogroup specific protective immunity in the RITARD model. In our present study, we advanced our research by formulating multi-serotype outer membrane vesicles, mixing the OMVs of five virulent V. cholerae strains. Four doses of oral immunization with cholera pentavalent outer membrane vesicles (CPMVs) induced V. cholerae specific B and T cell responses. CPMVs-immunized mice generated long lasting serum IgG, IgA, IgM as well as mucosal sIgA and also elicited a higher percentage of CD4+ T cell distribution in spleen. Our study revealed that in vitro CPMVs-activated dendritic cells were secreting T cell polarizing cytokines, IL-12p40, IL-4, IL-6 and IL-1β. Moreover, purified splenic CD4+ T cells of immunized mice also secreted IL-4, IL-13 and IL-17 cytokines, indicating the initiation of Th2 and Th17 cell mediated immune responses. CPMVs immunized adult female mice and their offspring were significantly protected from heterologous challenge with wild type V. cholerae. CPMVs could be exploited for the development of a novel non-living vaccine against circulating cholera in near future.
authors.affiliation: Division of Bacteriology, National Institute of Cholera and Enteric Diseases, P-33, CIT Road, Scheme XM, Beliaghata, Kolkata 700010, India.
authors.forename: Ritam
authors.initials: R
authors.lastname: Sinha
authors.affiliation: Division of Bacteriology, National Institute of Cholera and Enteric Diseases, P-33, CIT Road, Scheme XM, Beliaghata, Kolkata 700010, India. Electronic address: hemantakoley@hotmail.com.
authors.forename: Hemanta
authors.initials: H
authors.lastname: Koley
authors.affiliation: Division of Bacteriology, National Institute of Cholera and Enteric Diseases, P-33, CIT Road, Scheme XM, Beliaghata, Kolkata 700010, India.
authors.forename: Dhrubajyoti
authors.initials: D
authors.lastname: Nag
authors.affiliation: Division of Bacteriology, National Institute of Cholera and Enteric Diseases, P-33, CIT Road, Scheme XM, Beliaghata, Kolkata 700010, India.
authors.forename: Soma
authors.initials: S
authors.lastname: Mitra
authors.affiliation: Division of Bacteriology, National Institute of Cholera and Enteric Diseases, P-33, CIT Road, Scheme XM, Beliaghata, Kolkata 700010, India.
authors.forename: Asish K
authors.initials: AK
authors.lastname: Mukhopadhyay
authors.affiliation: Jadavpur University, 188, Raja S C Mullick Road, Kolkata 700032, India.
authors.forename: Brajadulal
authors.initials: B
authors.lastname: Chattopadhyay
mesh_terms: D056704:Adaptive Immunity / Q000276:immunology*; D000818:Animals; D000831:Animals, Newborn; D022121:Cholera Vaccines; D005260:Female; D053762:Interleukin-12 Subunit p40 / Q000276:immunology; D018793:Interleukin-13 / Q000276:immunology; D020381:Interleukin-17 / Q000276:immunology; D015847:Interleukin-4 / Q000276:immunology; D015850:Interleukin-6 / Q000276:immunology; D051379:Mice; D023421:Models, Animal; D017778:Vaccines, Combined / Q000276:immunology*; D014734:Vibrio cholerae / Q000276:immunology / Q000502:physiology*
chemical_list: D022121:Cholera Vaccines; D053762:Interleukin-12 Subunit p40; D018793:Interleukin-13; D020381:Interleukin-17; D015850:Interleukin-6; D017778:Vaccines, Combined; D015847:Interleukin-4
keywords: Adaptive immunity and passive protection; Cholera; Cholera pentavalent OMVs (CPMVs); Outer membrane vesicles (OMVs); Vibrio cholerae