title: Recombinant glycoprotein production in human cell lines.
abstract: The most important properties of a protein are determined by its primary structure, its amino acid sequence. However, protein features can be also modified by a large number of posttranslational modifications. These modifications can occur during or after the synthesis process, and glycosylation appears as the most common posttranslational modification. It is estimated that 50% of human proteins have some kind of glycosylation, which has a key role in maintaining the structure, stability, and function of the protein. Besides, glycostructures can also influence the pharmacokinetics and immunogenicity of the protein. Although the glycosylation process is a conserved mechanism that occurs in yeast, plants, and animals, several studies have demonstrated significant differences in the glycosylation pattern in recombinant proteins expressed in mammalian, yeast, and insect cells. Thus, currently, important efforts are being done to improve the systems for the expression of recombinant glycosylated proteins. Among the different mammalian cell lines used for the production of recombinant proteins, a significant difference in the glycosylation pattern that can alter the production and/or activity of the protein exists. In this context, human cell lines have emerged as a new alternative for the production of human therapeutic proteins, since they are able to produce recombinant proteins with posttranslational modifications similar to its natural counterpart and reduce potential immunogenic reactions against nonhuman epitopes. This chapter describes the steps necessary to produce a recombinant glycoprotein in a human cell line in small scale and also in bioreactors.
authors.affiliation: Department of Pharmaceutical Sciences, Faculty of Pharmaceutical Sciences of Ribeirão Preto-SP, University of São Paulo, Ribeirão Preto-SP, Brazil.
authors.forename: Kamilla
authors.initials: K
authors.lastname: Swiech
authors.forename: Marcela Cristina Corrêa
authors.initials: MC
authors.lastname: de Freitas
authors.forename: Dimas Tadeu
authors.initials: DT
authors.lastname: Covas
authors.forename: Virgínia
authors.initials: V
authors.lastname: Picanço-Castro
mesh_terms: D000818:Animals; D019149:Bioreactors; D002460:Cell Line / Q000378:metabolism*; D006023:Glycoproteins / Q000235:genetics* / Q000378:metabolism*; D006031:Glycosylation; D006801:Humans; D011499:Protein Processing, Post-Translational / Q000235:genetics; D011994:Recombinant Proteins / Q000235:genetics* / Q000378:metabolism*
chemical_list: D006023:Glycoproteins; D011994:Recombinant Proteins