title: Human parechovirus as a minor cause of acute otitis media in children.
abstract: Human parechoviruses (HPeVs) cause mild upper respiratory infections, gastrointestinal symptoms, central nervous system infections and some studies have linked them with acute otitis media (AOM). The aim of the present study was to study further the role of HPeV infections in AOM by detecting these viruses directly from middle ear fluid (MEF), respiratory and stool samples collected from children during AOM episodes. A total of 91 MEF samples, 98 nasal swab (NS) samples and 92 stool samples were collected during 100 AOM episodes in a total of 87 children aged between five to 42 months. All specimens were analyzed by real time RT-PCR for the presence of HPeV RNA. HPeV infection was diagnosed in 12 (14%) patients. HPeV RNA was detected in altogether 13 samples, including four MEF samples, three NS samples and six stool samples. One patient was positive in both stool and MEF samples. The results suggest that HPeV may play a role in some AOM cases, but it is not a major cause of AOM in children.
authors.affiliation: Department of Anatomy, School of Medicine, University of Tampere, 33520 Tampere, Finland; Department of Otorhinolaryngology, Head and Neck Surgery, Tampere University Hospital and School of Medicine, University of Tampere, 33520 Tampere, Finland. Electronic address: saara.sillanpaa@uta.fi.
authors.forename: Saara
authors.initials: S
authors.lastname: Sillanpää
authors.affiliation: Department of Virology, School of Medicine, University of Tampere, 33520 Tampere, Finland.
authors.forename: Sami
authors.initials: S
authors.lastname: Oikarinen
authors.affiliation: Department of Otorhinolaryngology, Head and Neck Surgery, Tampere University Hospital and School of Medicine, University of Tampere, 33520 Tampere, Finland.
authors.forename: Markku
authors.initials: M
authors.lastname: Sipilä
authors.affiliation: Department of Dermatology, Tampere University Hospital, 33520 Tampere, Finland.
authors.forename: Elina
authors.initials: E
authors.lastname: Seppälä
authors.affiliation: Department of Virology, School of Medicine, University of Tampere, 33520 Tampere, Finland.
authors.forename: Noora
authors.initials: N
authors.lastname: Nurminen
authors.affiliation: Department of Otorhinolaryngology, Head and Neck Surgery, Tampere University Hospital and School of Medicine, University of Tampere, 33520 Tampere, Finland.
authors.forename: Markus
authors.initials: M
authors.lastname: Rautiainen
authors.affiliation: Department of Otorhinolaryngology, Head and Neck Surgery, Tampere University Hospital and School of Medicine, University of Tampere, 33520 Tampere, Finland.
authors.forename: Jussi
authors.initials: J
authors.lastname: Laranne
authors.affiliation: Department of Virology, School of Medicine, University of Tampere, 33520 Tampere, Finland; Fimlab Laboratories, Pirkanmaa Hospital District, 33520 Tampere, Finland.
authors.forename: Heikki
authors.initials: H
authors.lastname: Hyöty
mesh_terms: D000293:Adolescent; D000328:Adult; D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D008297:Male; D010033:Otitis Media / Q000175:diagnosis / Q000453:epidemiology* / Q000382:microbiology / Q000821:virology*; D030061:Parechovirus / Q000235:genetics; D010850:Picornaviridae Infections / Q000175:diagnosis / Q000453:epidemiology*; D012307:Risk Factors; D012621:Seasons; D055815:Young Adult
keywords: Acute otitis media; Child; Human parechovirus; Infection; Picornavirus; Polymerase chain reaction