title: Fibrinolytic, anti-inflammatory and anti-microbial properties of α-(1-3)-glucans produced from Streptococcus mutans (MTCC 497).
abstract: Streptococcus mutans (MTCC 497) cell associated α-(1-3)-glucans were isolated, characterized and evaluated for their bioactivity profile. Acid hydrolysis of α-(1-3)-glucans revealed presence of glucose moieties. Water insoluble α-(1-3)-glucans (WIG) were sulfated to convert them into water soluble glucans which were characterized by FT-IR spectral studies. The sulfation of WIG was confirmed by the presence of -O-SO3- and C-O-SO3- characteristic peaks at 1240 and 820 cm(-1). MALDI-TOF analysis of sulfated α-(1-3)-glucan revealed 1.2 to 9kDa fragmentation. Antibacterial profile studies revealed higher growth inhibitory activity against Gram negative than Gram positive bacterial strains by sulfated α-(1-3)-glucans. One-fold higher anti-inflammatory activity with IC50 value of 0.11mg/ml was observed with sulfated α-(1-3)-glucans over WIG. Time dependent fibrinolytic potential without requirement of tissue plasminogen activators was observed for sulfated α-(1-3)-glucans. This is the first report demonstrating the fibrinolytic and anti-inflammatory property for sulfated α-(1-3)-glucans.
authors.affiliation: Bioengineering and Environmental Sciences, Indian Institute of Chemical Technology, Habsiguda, Hyderabad 500007, India; Academy of Scientific and Innovative Research, New Delhi, India.
authors.forename: Sudheer Kumar
authors.initials: SK
authors.lastname: Buddana
authors.affiliation: Bioengineering and Environmental Sciences, Indian Institute of Chemical Technology, Habsiguda, Hyderabad 500007, India.
authors.forename: Yaswanth Venkata Naga
authors.initials: YV
authors.lastname: Varanasi
authors.affiliation: Bioengineering and Environmental Sciences, Indian Institute of Chemical Technology, Habsiguda, Hyderabad 500007, India; Academy of Scientific and Innovative Research, New Delhi, India. Electronic address: prakasam@iict.res.in.
authors.forename: Prakasham Reddy
authors.initials: PR
authors.lastname: Shetty
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects*; D002417:Cattle; D004305:Dose-Response Relationship, Drug; D005342:Fibrinolysis / Q000187:drug effects*; D005343:Fibrinolytic Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D005936:Glucans / Q000096:biosynthesis / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D006801:Humans; D008826:Microbial Sensitivity Tests; D012710:Serum Albumin, Bovine / Q000037:antagonists & inhibitors; D013295:Streptococcus mutans / Q000737:chemistry* / Q000378:metabolism; D013329:Structure-Activity Relationship
chemical_list: D000900:Anti-Bacterial Agents; D000894:Anti-Inflammatory Agents, Non-Steroidal; D005343:Fibrinolytic Agents; D005936:Glucans; D012710:Serum Albumin, Bovine
keywords: Anti-inflammatory; Antibacterial; Fibrinolytic; Streptococcus mutans; Sulfated α-(1-3)-glucans; α-(1-3)-Glucans