title: A GCC-box motif in the promoter of nudix hydrolase 7 (AtNUDT7) gene plays a role in ozone response of Arabidopsis ecotypes.
abstract: Arabidopsis nudix hydrolase 7 (AtNudt7) plays an important role in regulating redox homeostasis during stress/defense signaling and seed germination. The early responsiveness of AtNudt7 provides a useful marker especially during oxidative cell death in plants. Nuclear run-on assays demonstrate that AtNudt7 is transcriptionally regulated. AtNUDT7 promoter-GUS transgenic plants show rapid inducibility in response to ozone and pathogens. A 16-bp insertion containing a GCC-box motif was identified in the promoter of a Ws-2 ecotype and was absent in Col-0. The 16-bp sequence was identified in 5% of the Arabidopsis ecotypes used in the 1001 genome sequencing project. The kinetics of expression of Ethylene Response Factor 1 (ERF1), a GCC-box binding factor is in synchrony with expression of AtNudt7 in response to ozone stress. ERF1 protein binds to the GCC-box motif in the AtNUDT7 promoter. In silico analysis of erf1 mutant and overexpressor lines supports a role for this protein in regulating AtNUDT7 expression.
authors.affiliation: Department of Biochemistry and Molecular Biology, Oklahoma State University, Stillwater, OK 74078, USA.
authors.forename: Meenakumari
authors.initials: M
authors.lastname: Muthuramalingam
authors.affiliation: Department of Biochemistry and Molecular Biology, Oklahoma State University, Stillwater, OK 74078, USA.
authors.forename: Xin
authors.initials: X
authors.lastname: Zeng
authors.affiliation: Department of Biochemistry and Molecular Biology, Oklahoma State University, Stillwater, OK 74078, USA.
authors.forename: Niranjani J
authors.initials: NJ
authors.lastname: Iyer
authors.affiliation: Department of Biological Sciences, Louisiana State University, Baton Rouge, LA 70803, USA.
authors.forename: Peter
authors.initials: P
authors.lastname: Klein
authors.affiliation: Department of Biochemistry and Molecular Biology, Oklahoma State University, Stillwater, OK 74078, USA. Electronic address: ramamurthy.mahalingam@okstate.edu.
authors.forename: Ramamurthy
authors.initials: R
authors.lastname: Mahalingam
mesh_terms: D017360:Arabidopsis / Q000145:classification / Q000201:enzymology* / Q000235:genetics / Q000502:physiology; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D003198:Computer Simulation; D060146:Ecotype; D018506:Gene Expression Regulation, Plant; D059372:Nucleotide Motifs; D010083:Oxidants, Photochemical / Q000494:pharmacology*; D010126:Ozone / Q000494:pharmacology*; D010454:Peptide Termination Factors / Q000378:metabolism*; D030821:Plants, Genetically Modified / Q000201:enzymology / Q000235:genetics; D011401:Promoter Regions, Genetic; D011755:Pyrophosphatases / Q000235:genetics* / Q000378:metabolism; D000096964:Nudix Hydrolases
chemical_list: D029681:Arabidopsis Proteins; D010083:Oxidants, Photochemical; D010126:Ozone; D010454:Peptide Termination Factors; D011755:Pyrophosphatases; D000096964:Nudix Hydrolases; C494978:eukaryotic release factor 1, Arabidopsis
keywords: AtNUDT7; Ethylene Response Factor 1; GCC box motif; Oxidative stress; Promoter