title: Hydrogen-rich saline resuscitation alleviates inflammation induced by severe burn with delayed resuscitation.
abstract: Severe burns with delayed resuscitation are associated with high morbidity which is attributed to ischemia-reperfusion injury. This study was undertaken to investigate the effect of hydrogen-rich saline known as a significant selective antioxidant on the inflammatory reaction induced by severe burns with delayed resuscitation. By establishing the model of severe burns with delayed resuscitation in rats, we recorded improvement on the mortality, secretion of cytokines and reaction of oxidative stress of rats treated with hydrogen-rich saline. We found that resuscitation by hydrogen-rich saline alleviated inflammation significantly. We further detected the change of the key nuclear factor NF-κB contributed to inflammation. The expression of both NF-κB and phosphorylated NF-κB in rats having severe burns with delayed resuscitation by hydrogen-rich saline was lower than that in rats with delayed resuscitation with Ringers' solution. Our data imply that hydrogen-rich saline significantly improves the inflammatory reaction in rats with severe burns with delayed resuscitation, possibly by inhibiting activation of NF-κB.
authors.affiliation: Department of Burn and Plastic Surgery, Chengdu Military General Hospital, No. 270, Rongdu Avenue, Jinniu District, Chengdu, Sichuan 610083, PR China. Electronic address: oasiswxj@163.com.
authors.forename: Xiaojuan
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Burn and Plastic Surgery, Jinling Hospital, School of Medicine, Nanjing University, Nanjing 210002, PR China.
authors.forename: Pan
authors.initials: P
authors.lastname: Yu
authors.affiliation: Department of Burn and Plastic Surgery, Chengdu Military General Hospital, No. 270, Rongdu Avenue, Jinniu District, Chengdu, Sichuan 610083, PR China.
authors.lastname: YongYang
authors.affiliation: Department of Digestive Diseases, Chengdu Military General Hospital, No. 270, Rongdu Avenue, Jinniu District, Chengdu, Sichuan 610083, PR China.
authors.forename: Xiaocong
authors.initials: X
authors.lastname: Liu
authors.affiliation: Department of Burn and Plastic Surgery, Chengdu Military General Hospital, No. 270, Rongdu Avenue, Jinniu District, Chengdu, Sichuan 610083, PR China.
authors.forename: Jinheng
authors.initials: J
authors.lastname: Jiang
authors.affiliation: Department of Burn and Plastic Surgery, Chengdu Military General Hospital, No. 270, Rongdu Avenue, Jinniu District, Chengdu, Sichuan 610083, PR China.
authors.forename: Degui
authors.initials: D
authors.lastname: Liu
authors.affiliation: Department of Burn and Plastic Surgery, Chengdu Military General Hospital, No. 270, Rongdu Avenue, Jinniu District, Chengdu, Sichuan 610083, PR China. Electronic address: xuegang1971@163.com.
authors.forename: Gang
authors.initials: G
authors.lastname: Xue
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D000704:Analysis of Variance; D000818:Animals; D000975:Antioxidants / Q000378:metabolism / Q000494:pharmacology*; D002056:Burns / Q000150:complications* / Q000378:metabolism / Q000628:therapy; D016207:Cytokines / Q000378:metabolism; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000378:metabolism; D004195:Disease Models, Animal; D005440:Fluid Therapy / Q000379:methods*; D007249:Inflammation / Q000188:drug therapy* / Q000209:etiology; D018836:Inflammation Mediators / Q000378:metabolism; D008297:Male; D009000:Monocytes / Q000378:metabolism; D016328:NF-kappa B / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D012151:Resuscitation / Q000379:methods*; D012769:Shock / Q000188:drug therapy / Q000209:etiology / Q000378:metabolism; D012965:Sodium Chloride / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; D016207:Cytokines; D018836:Inflammation Mediators; D016328:NF-kappa B; D012965:Sodium Chloride; D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine
keywords: Delayed resuscitation; Hydrogen-rich saline resuscitation; Inflammatory reaction; Severe burns