title: Serial coupling of ion-exchange and size-exclusion chromatography to determine aggregation levels in mAbs in the presence of a proteinaceous excipient, recombinant human serum albumin.
abstract: Aggregation levels of therapeutic proteins may be difficult to determine in mixtures containing other proteinaceous excipients. We performed a feasibility study of using serial coupling of an anion exchange and size exclusion column to determine the aggregation levels of four different model monoclonal antibodies (mAb) mixed with the model proteinaceous excipient recombinant human serum albumin (rHSA). For three of the four mAbs suitable elution conditions could be established. From the limitations imposed by the pI of the rHSA, the pI of the mAb and the nature of the columns used, it was possible to propose a set of general conditions that allows quantification of the aggregation level of a therapeutic protein in the presence of a proteinaceous excipient: The excipient protein and protein of interest should differ in pI by a minimum of 0.5 units, and the pI of the protein of interest should not be higher than ca. 8.5.
authors.affiliation: Novozymes Biopharma, Bagsvaerd, 2880, Denmark.
authors.forename: Paul Luigi Gargani
authors.initials: PL
authors.lastname: Weisbjerg
authors.forename: Mikael Bjerg
authors.initials: MB
authors.lastname: Caspersen
authors.forename: Ken
authors.initials: K
authors.lastname: Cook
authors.forename: Marco
authors.initials: M
authors.lastname: Van De Weert
mesh_terms: D000911:Antibodies, Monoclonal / Q000032:analysis*; D002850:Chromatography, Gel / Q000379:methods*; D002852:Chromatography, Ion Exchange / Q000379:methods; D005079:Excipients / Q000032:analysis*; D006801:Humans; D066329:Protein Aggregates; D011994:Recombinant Proteins / Q000032:analysis; D012709:Serum Albumin / Q000032:analysis*
chemical_list: D000911:Antibodies, Monoclonal; D005079:Excipients; D066329:Protein Aggregates; D011994:Recombinant Proteins; D012709:Serum Albumin
keywords: HPLC (high-performance/pressure liquid chromatography); albumin; analysis; chromatography; formulation; monoclonal antibody; protein aggregation; protein formulation