title: Structure and rheological properties of a xyloglucan extracted from Hymenaea courbaril var. courbaril seeds.
abstract: Hymenaea courbaril var courbaril seed xyloglucan was efficiently extracted with 0.1M NaCl, followed by ethanol precipitation (yield=72±5% w/w). Its amorphous structure was identified by the pattern of X-ray diffraction. The monosaccharide composition was determined by GC/MS analysis of the alditol acetates and showed the occurrence of glucose:xylose:galactose:arabinose (40:34:20:6). One-(1D) and two-dimensional-(2D) NMR spectra confirmed a central backbone composed by 4-linked β-glucose units partially branched at position 6 with non-reducing terminal units of α-xylose or β-galactose-(1→2)-α-xylose disaccharides. The xyloglucan solution was evaluated by dynamic light scattering and presents a polydisperse and practically neutral profile, and at 0.5 and 1.0% (w/v) the solutions behave as a viscoelastic fluid. The polysaccharide did not show significant antibacterial or hemolytic activities. Overall our results indicate that xyloglucan from H. courbaril is a promising polysaccharide for food and pharmaceutical industries.
authors.affiliation: Departamento de Bioquímica, Universidade Federal de Pernambuco-UFPE, Av. Prof. Moraes Rego s/n, CEP: 50.670-420, Recife, PE, Brasil.
authors.forename: Isabel R S
authors.initials: IR
authors.lastname: Arruda
authors.affiliation: Departamento de Bioquímica, Universidade Federal de Pernambuco-UFPE, Av. Prof. Moraes Rego s/n, CEP: 50.670-420, Recife, PE, Brasil; Laboratório de Imunopatologia Keizo Asami-LIKA/UFPE.
authors.forename: Priscilla B S
authors.initials: PB
authors.lastname: Albuquerque
authors.affiliation: Laboratório de Tecido Conjuntivo, Hospital Universitário Clementino Fraga Filho, Programa de Glicobiologia, Instituto de Bioquímica Médica, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brasil.
authors.forename: Gustavo R C
authors.initials: GR
authors.lastname: Santos
authors.affiliation: Departamento de Bioquímica, Universidade Federal de Pernambuco-UFPE, Av. Prof. Moraes Rego s/n, CEP: 50.670-420, Recife, PE, Brasil.
authors.forename: Alexandre G
authors.initials: AG
authors.lastname: Silva
authors.affiliation: Laboratório de Tecido Conjuntivo, Hospital Universitário Clementino Fraga Filho, Programa de Glicobiologia, Instituto de Bioquímica Médica, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brasil.
authors.forename: Paulo A S
authors.initials: PA
authors.lastname: Mourão
authors.affiliation: Departamento de Bioquímica, Universidade Federal de Pernambuco-UFPE, Av. Prof. Moraes Rego s/n, CEP: 50.670-420, Recife, PE, Brasil.
authors.forename: Maria T S
authors.initials: MT
authors.lastname: Correia
authors.affiliation: CEB-Centre of Biological Engineering, Universidade do Minho, Campus de Gualtar, 4710-057 Braga, Portugal.
authors.forename: António A
authors.initials: AA
authors.lastname: Vicente
authors.affiliation: Departamento de Bioquímica, Universidade Federal de Pernambuco-UFPE, Av. Prof. Moraes Rego s/n, CEP: 50.670-420, Recife, PE, Brasil; Laboratório de Imunopatologia Keizo Asami-LIKA/UFPE. Electronic address: mgcc@ufpe.br.
authors.forename: Maria G
authors.initials: MG
authors.lastname: Carneiro-da-Cunha
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology; D002850:Chromatography, Gel; D005936:Glucans / Q000737:chemistry* / Q000494:pharmacology; D053878:Hemolytic Agents / Q000737:chemistry / Q000494:pharmacology; D052597:Hymenaea / Q000737:chemistry*; D008745:Methylation; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D010936:Plant Extracts / Q000737:chemistry* / Q000494:pharmacology; D012212:Rheology; D012639:Seeds / Q000737:chemistry*; D014961:X-Ray Diffraction; D014990:Xylans / Q000737:chemistry* / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D005936:Glucans; D053878:Hemolytic Agents; D010936:Plant Extracts; D014990:Xylans; C029353:xyloglucan
keywords: Hymenaea courbaril; Polysaccharide characterization; Polysaccharide structure; Viscoelastic fluid; Xyloglucan