title: Mitochondrial protein turnover: methods to measure turnover rates on a large scale.
abstract: Mitochondrial proteins carry out diverse cellular functions including ATP synthesis, ion homeostasis, cell death signaling, and fatty acid metabolism and biogenesis. Compromised mitochondrial quality control is implicated in various human disorders including cardiac diseases. Recently it has emerged that mitochondrial protein turnover can serve as an informative cellular parameter to characterize mitochondrial quality and uncover disease mechanisms. The turnover rate of a mitochondrial protein reflects its homeostasis and dynamics under the quality control systems acting on mitochondria at a particular cell state. This review article summarizes some recent advances and outstanding challenges for measuring the turnover rates of mitochondrial proteins in health and disease. This article is part of a Special Issue entitled "Mitochondria: From Basic Mitochondrial Biology to Cardiovascular Disease".
authors.affiliation: The NHLBI Proteomics Center at UCLA, Los Angeles, CA 90095, USA; Department of Physiology, UCLA David Geffen School of Medicine, Los Angeles, CA 90095, USA.
authors.forename: X'avia C Y
authors.initials: XC
authors.lastname: Chan
authors.affiliation: The NHLBI Proteomics Center at UCLA, Los Angeles, CA 90095, USA; Department of Physiology, UCLA David Geffen School of Medicine, Los Angeles, CA 90095, USA.
authors.forename: Caitlin M
authors.initials: CM
authors.lastname: Black
authors.affiliation: The NHLBI Proteomics Center at UCLA, Los Angeles, CA 90095, USA; Department of Physiology, UCLA David Geffen School of Medicine, Los Angeles, CA 90095, USA.
authors.forename: Amanda J
authors.initials: AJ
authors.lastname: Lin
authors.affiliation: The NHLBI Proteomics Center at UCLA, Los Angeles, CA 90095, USA; Department of Physiology, UCLA David Geffen School of Medicine, Los Angeles, CA 90095, USA; Department of Medicine, UCLA David Geffen School of Medicine, Los Angeles, CA 90095, USA; Department of Bioinformatics, UCLA David Geffen School of Medicine, Los Angeles, CA 90095, USA. Electronic address: pping@mednet.ucla.edu.
authors.forename: Peipei
authors.initials: P
authors.lastname: Ping
authors.affiliation: The NHLBI Proteomics Center at UCLA, Los Angeles, CA 90095, USA; Department of Physiology, UCLA David Geffen School of Medicine, Los Angeles, CA 90095, USA. Electronic address: edwardlau@ucla.edu.
authors.forename: Edward
authors.initials: E
authors.lastname: Lau
mesh_terms: D000818:Animals; D006801:Humans; D008928:Mitochondria / Q000378:metabolism*; D024101:Mitochondrial Proteins / Q000378:metabolism*; D020543:Proteome; D040901:Proteomics / Q000379:methods
chemical_list: D024101:Mitochondrial Proteins; D020543:Proteome
keywords: Heavy water; Mitochondria; Protein turnover; Proteome dynamics; Proteomics