title: Peritrophic membrane origin in adult bees (Hymenoptera): immunolocalization.
abstract: The midgut is a region of the digestive tract of bees with the lumen lined by a peritrophic membrane that is composed of chitin and proteins (peritrophins). The origin of the peritrophins in the midgut of adult bees is unknown. This study used an anti-peritrophin 55-kDa antibody to immunolocalize the sites of the peritrophic membrane synthesis in nine species of adult bees' representatives of different families and sociability levels. In all studied species the peritrophin-55 is produced by digestive cells in the entire midgut in the rough endoplasmic reticulum following transference to Golgi apparatus and released by secretory vesicles, which fuses with the plasma membrane and microvilli. Thus, in the representatives of different groups of bees, the PM is of type I.
authors.affiliation: Departamento de Biologia Geral, Universidade Federal de Viçosa, 36570-000 Viçosa, MG, Brazil.
authors.forename: Aparecida das Dores
authors.initials: ADD
authors.lastname: Teixeira
authors.affiliation: Departamento de Entomologia, Universidade Federal de Viçosa, 36570-000 Viçosa, MG, Brazil.
authors.forename: Solange
authors.initials: S
authors.lastname: Marques-Araújo
authors.affiliation: Departamento de Entomologia, Universidade Federal de Viçosa, 36570-000 Viçosa, MG, Brazil.
authors.forename: José Cola
authors.initials: JC
authors.lastname: Zanuncio
authors.affiliation: Departamento de Biologia Geral, Universidade Federal de Viçosa, 36570-000 Viçosa, MG, Brazil. Electronic address: jeserrao@ufv.br.
authors.forename: José Eduado
authors.initials: JE
authors.lastname: Serrão
mesh_terms: D000818:Animals; D001516:Bees / Q000033:anatomy & histology*; D041981:Gastrointestinal Tract / Q000033:anatomy & histology / Q000737:chemistry; D007150:Immunohistochemistry; D019476:Insect Proteins / Q000032:analysis*; D008566:Membranes / Q000033:anatomy & histology* / Q000737:chemistry
chemical_list: D019476:Insect Proteins
keywords: Apidae; Immunocytochemistry; Midgut; Peritrophin