title: Extraction and isolation of alkaloids of Sophora alopecuroides and their anti-tumor effects in H22 tumor-bearing mice.
abstract: BACKGROUND
  Alkaloids of Sophora alopecuroides have good biological activity, and are widely used in clinical settings, which not only have pharmacological activities of anti-cancer, cancer suppression, as well as the inhibition, and killing of various microorganisms; but also possess extensive pharmacological effects on immune system, nervous system and cardiovascular system. The objective of this paper was to extract and isolate total alkaloids of Sophora alopecuroides (TASA), and to study their anti-tumor effects in H22 tumor-bearing mice.
  
  
  METHODS
  TASA were extracted and isolated using thin-layer chromatography, and column chromatography; and the isolated compounds were analyzed using nuclear magnetic resonance. The inhibitory effects of TASA on tumor in H22-bearing mice were determined by MTT assay.
  
  
  RESULTS
  Three compounds were isolated from Sophora alopecuroides L., which were matrine, oxymatrine and sophoridine, respectively. Meanwhile, mouse H22 sarcoma model was established and different doses of TASA apparently inhibited solid H22-tumor in mice; it inhibited the thymus, and spleen to some extent; the degree of inhibition was more obvious for the spleen.
  
  
  CONCLUSIONS
  TASA has an anti-tumor effect in H22 tumor-bearing mice.
authors.affiliation: Affiliated Hospital of Logistics College of the CAPF, Tianjin 300162, China.
authors.forename: AiHua
authors.initials: A
authors.lastname: Chang
authors.affiliation: Tianjin First Centre Hospital,Tianjin,300192,China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Cai
authors.affiliation: Affiliated Hospital of Logistics College of the CAPF, Tianjin 300162, China.
authors.forename: ZhiHong
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Affiliated Hospital of Logistics College of the CAPF, Tianjin 300162, China.
authors.forename: ShengKai
authors.initials: S
authors.lastname: Sun
mesh_terms: D000470:Alkaloids / Q000008:administration & dosage* / Q000032:analysis / Q000302:isolation & purification; D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000008:administration & dosage* / Q000302:isolation & purification; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D005260:Female; D006801:Humans; D008297:Male; D051379:Mice; D009369:Neoplasms / Q000188:drug therapy* / Q000503:physiopathology; D010936:Plant Extracts / Q000008:administration & dosage* / Q000032:analysis / Q000302:isolation & purification; D029909:Sophora / Q000737:chemistry*; D023041:Xenograft Model Antitumor Assays
chemical_list: D000470:Alkaloids; D000972:Antineoplastic Agents, Phytogenic; D010936:Plant Extracts
keywords: H22 tumor-bearing mice; Sophora alopecuroides L.; matrine; oxymatrine and sophoridine