title: Transcriptomics expression analysis to unveil the molecular mechanisms underlying the cocoa polyphenol treatment in diet-induced obesity rats.
abstract: Cocoa polyphenol (CP), due to their biological actions, may be supplementary treatments for adipose tissue-fat gain. However, the molecular mechanism of CPs is still ambiguous. This study investigated the hypothesis that CP treatment modulates expressing of lipid metabolism genes in mesenteric white adipose tissue (MES-WAT). Sprague-Dawley (SD) rats were fed a low-fat (LF) or high-fat (HF) diet for 12 weeks. Thereafter, HFD rats (n = 10/group) were treated at a dose of 600 mg/kg bw/day CPs (HFD + CPs) for 4 weeks. DNA microarray analysis resulted in 753 genes of the 13,008 genes expressed. Bioinformatics tools showed CP treatment significantly decreased gene expression levels for lipogenic enzymes, while increased the mRNA levels responsible for lipolysis enzymes. CP administration differentially regulates gene expression involved in lipid metabolism in MES-WAT. These data unveil a new insight into the molecular mechanisms underlying the pharmacological effect of CPs on obesity biomarkers in obese rats.
authors.affiliation: Department of Nutrition and Dietetics, Metabolism and Genomics Group, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Selangor, Malaysia.
authors.forename: Faisal
authors.initials: F
authors.lastname: Ali
authors.affiliation: Department of Nutrition and Dietetics, Metabolism and Genomics Group, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Selangor, Malaysia. Electronic address: amin@medic.upm.edu.my.
authors.forename: Amin
authors.initials: A
authors.lastname: Ismail
authors.affiliation: Department of Nutrition and Dietetics, Metabolism and Genomics Group, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Selangor, Malaysia; Laboratory of Molecular Biomedicine, Institute of Bioscience, Universiti Putra Malaysia, Selangor 43400, Malaysia.
authors.forename: Norhaizan Mohd
authors.initials: NM
authors.lastname: Esa
authors.affiliation: Department of Biomedical Sciences, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
authors.forename: Chong Pei
authors.initials: CP
authors.lastname: Pei
mesh_terms: D052436:Adipose Tissue, White / Q000378:metabolism; D000818:Animals; D002099:Cacao / Q000737:chemistry*; D018752:Diet, Fat-Restricted; D059305:Diet, High-Fat; D004195:Disease Models, Animal; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000187:drug effects*; D006801:Humans; D050356:Lipid Metabolism / Q000187:drug effects*; D008297:Male; D009765:Obesity / Q000188:drug therapy* / Q000209:etiology / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology; D059808:Polyphenols / Q000008:administration & dosage* / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D010936:Plant Extracts; D059808:Polyphenols
keywords: Cocoa polyphenol; DNA microarray; Lipid metabolism; MES-WAT; Obesity; Transcriptomics