title: Economically enhanced succinic acid fermentation from cassava bagasse hydrolysate using Corynebacterium glutamicum immobilized in porous polyurethane filler.
abstract: An immobilized fermentation system, using cassava bagasse hydrolysate (CBH) and mixed alkalis, was developed to achieve economical succinic acid production by Corynebacterium glutamicum. The C. glutamicum strains were immobilized in porous polyurethane filler (PPF). CBH was used efficiently as a carbon source instead of more expensive glucose. Moreover, as a novel method for regulating pH, the easily decomposing NaHCO3 was replaced by mixed alkalis (NaOH and Mg(OH)2) for succinic acid production by C. glutamicum. Using CBH and mixed alkalis in the immobilized batch fermentation system, succinic acid productivity of 0.42gL(-1)h(-1) was obtained from 35gL(-1) glucose of CBH, which is similar to that obtained with conventional free-cell fermentation with glucose and NaHCO3. In repeated batch fermentation, an average of 22.5gL(-1) succinic acid could be obtained from each batch, which demonstrated the enhanced stability of the immobilized C. glutamicum cells.
authors.affiliation: State Key Laboratory of Materials-Oriented Chemical Engineering, College of Life Science and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing 210009, PR China.
authors.forename: Xinchi
authors.initials: X
authors.lastname: Shi
authors.affiliation: State Key Laboratory of Materials-Oriented Chemical Engineering, College of Life Science and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing 210009, PR China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Materials-Oriented Chemical Engineering, College of Life Science and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing 210009, PR China.
authors.forename: Hengfei
authors.initials: H
authors.lastname: Ren
authors.affiliation: State Key Laboratory of Materials-Oriented Chemical Engineering, College of Life Science and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing 210009, PR China.
authors.forename: Dong
authors.initials: D
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Materials-Oriented Chemical Engineering, College of Life Science and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing 210009, PR China.
authors.forename: Ting
authors.initials: T
authors.lastname: Zhao
authors.affiliation: State Key Laboratory of Materials-Oriented Chemical Engineering, College of Life Science and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing 210009, PR China.
authors.forename: Nan
authors.initials: N
authors.lastname: Zhao
authors.affiliation: State Key Laboratory of Materials-Oriented Chemical Engineering, College of Life Science and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing 210009, PR China. Electronic address: yinghanjie@njtech.edu.cn.
authors.forename: Hanjie
authors.initials: H
authors.lastname: Ying
mesh_terms: D000693:Anaerobiosis; D061249:Batch Cell Culture Techniques; D001709:Biotechnology / Q000191:economics* / Q000379:methods*; D002241:Carbohydrates / Q000032:analysis; D002244:Carbon / Q000494:pharmacology; D018914:Cells, Immobilized / Q000187:drug effects / Q000378:metabolism; D002482:Cellulose / Q000378:metabolism*; D048230:Corynebacterium glutamicum / Q000166:cytology* / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D005285:Fermentation / Q000187:drug effects; D005947:Glucose / Q000378:metabolism; D006868:Hydrolysis / Q000187:drug effects; D008276:Magnesium Hydroxide / Q000494:pharmacology; D002365:Manihot / Q000187:drug effects / Q000378:metabolism*; D053858:Metabolic Networks and Pathways / Q000187:drug effects; D011140:Polyurethanes / Q000494:pharmacology*; D016062:Porosity; D012972:Sodium Hydroxide / Q000494:pharmacology; D019802:Succinic Acid / Q000378:metabolism*
chemical_list: D002241:Carbohydrates; D011140:Polyurethanes; D012972:Sodium Hydroxide; D002244:Carbon; D002482:Cellulose; C027433:bagasse; D019802:Succinic Acid; D005947:Glucose; D008276:Magnesium Hydroxide
keywords: Cassava bagasse hydrolysate; Corynebacterium glutamicum; Mixed alkaline neutralizers; Porous polyurethane filler (PPF); Succinic acid