title: A thermoresponsive and magnetic colloid for 3D cell expansion and reconfiguration.
abstract: A dual thermoresponsive and magnetic colloidal gel matrix is described for enhanced stem-cell culture. The combined properties of the material allow enzyme-free passaging and expansion of mesenchymal stem cells, as well as isolation of cells postculture by the simple process of lowering the temperature and applying an external magnetic field. The colloidal gel can be reconfigured with thermal and magnetic stimuli to allow patterning of cells in discrete zones and to control movement of cells within the porous matrix during culture.
authors.affiliation: School of Pharmacy, University of East Anglia, Norwich, NR4 7TJ, UK.
authors.forename: Aram
authors.initials: A
authors.lastname: Saeed
authors.forename: Nora
authors.initials: N
authors.lastname: Francini
authors.forename: Lisa
authors.initials: L
authors.lastname: White
authors.forename: James
authors.initials: J
authors.lastname: Dixon
authors.forename: Toby
authors.initials: T
authors.lastname: Gould
authors.forename: Hassan
authors.initials: H
authors.lastname: Rashidi
authors.forename: Racha Cheikh
authors.initials: RC
authors.lastname: Al Ghanami
authors.forename: Veronika
authors.initials: V
authors.lastname: Hruschka
authors.forename: Heinz
authors.initials: H
authors.lastname: Redl
authors.forename: Brian R
authors.initials: BR
authors.lastname: Saunders
authors.forename: Cameron
authors.initials: C
authors.lastname: Alexander
authors.forename: Kevin M
authors.initials: KM
authors.lastname: Shakesheff
mesh_terms: D018929:Cell Culture Techniques / Q000379:methods*; D049109:Cell Proliferation; D003102:Colloids; D006801:Humans; D060328:Magnetic Phenomena; D059630:Mesenchymal Stem Cells / Q000166:cytology; D013696:Temperature
chemical_list: D003102:Colloids
keywords: 3D scaffolds; biomaterials; magnetic particles; stem cells; thermoresponsive polymers