title: Structural characterization, sulfation and antitumor activity of a polysaccharide fraction from Cyclina sinensis.
abstract: In the present study, we investigated the preliminary structure, sulfation and antitumor activity of a polysaccharide fraction from Cyclina sinensis (CSPS-1). Results of structural characterization showed that the backbone chain of CSPS-1 was composed of glucose linked by α-(1→4) glycosidic bond, and the branch chain was attached to backbone chain by (1→6) glycosidic bond. CSPS-1 was sulfated by chlorosulfonic acid-pyridine method under different modification conditions according to the orthogonal test L9(3(4)), affording nine sulfated polysaccharides (CSPS-1-S). The optimal sulfation conditions for CSPS-1 were reaction temperature of 65°C, reaction time of 2h and chlorosulfonic acid-pyridine ratio of 1:4. Structural analysis revealed that sulfation had occurred at position of C-6 in CSPS-1. In addition, CSPS-1-S exhibited significantly higher inhibitory activity in vitro against human gastric cancer BGC-823 cells.
authors.affiliation: College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, Jiangsu, PR China; School of Life Science and Chemical Engineering, Huaiyin Institute of Technology, Huaian 223003, Jiangsu, PR China.
authors.forename: Changxing
authors.initials: C
authors.lastname: Jiang
authors.affiliation: School of Life Science and Chemical Engineering, Huaiyin Institute of Technology, Huaian 223003, Jiangsu, PR China.
authors.forename: Qingping
authors.initials: Q
authors.lastname: Xiong
authors.affiliation: School of Life Science and Chemical Engineering, Huaiyin Institute of Technology, Huaian 223003, Jiangsu, PR China.
authors.forename: Songlin
authors.initials: S
authors.lastname: Li
authors.affiliation: School of Life Science and Chemical Engineering, Huaiyin Institute of Technology, Huaian 223003, Jiangsu, PR China.
authors.forename: Xirong
authors.initials: X
authors.lastname: Zhao
authors.affiliation: College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, Jiangsu, PR China. Electronic address: zengxx@njau.edu.cn.
authors.forename: Xiaoxiong
authors.initials: X
authors.lastname: Zeng
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry* / Q000494:pharmacology*; D049872:Bivalvia / Q000737:chemistry*; D045744:Cell Line, Tumor; D006801:Humans; D011134:Polysaccharides / Q000737:chemistry* / Q000494:pharmacology*; D013431:Sulfates / Q000737:chemistry*
chemical_list: D000970:Antineoplastic Agents; D011134:Polysaccharides; D013431:Sulfates
keywords: Antitumor activity; Cyclina sinensis; Polysaccharide; Structural characterization; Sulfation