title: The postnatal origin of adult neural stem cells and the effects of glucocorticoids on their genesis.
abstract: The relevance of adult neurogenesis in hippocampal function is well documented, as is the potential impact stress has on the adult neurogenic niche. Adult born neurons are generated from neural precursors in the dentate gyrus (DG), although the point in postnatal development that these cell precursors originate is not known. This is particularly relevant if we consider the effects stress may have on the development of neural precursors, and whether such effects on adult neurogenesis and behavior may persist in the long-term. We have analyzed the proportion of neural precursors in the adult murine hippocampus born on specific days during postnatal development using a dual birth-dating analysis, and we assessed their sensitivity to dexamethasone (DEX) on the peak day of cell generation. We also studied the consequences of postnatal DEX administration on adult hippocampal-dependent behavior. Postnatal day 6 (P6) is a preferred period for proliferating neural stem cells (NSCs) to become the precursors that remain in a proliferative state throughout adulthood. This window is independent of gender, the cell's location in the DG granule cell layer or their rostro-caudal position. DEX administration at P6 reduces the size of the adult NSC pool in the DG, which is correlated with poor learning/memory capacity and increased anxiety-like behavior. These results indicate that aNSCs are generated non-uniformly during postnatal development, with peak generation on day P6, and that stress receptor activation during the key period of postnatal NSC generation has a profound impact on both adult hippocampal neurogenesis and behavior.
authors.affiliation: Molecular, Cellular and Developmental Neurobiology Department, Cajal Institute, CSIC, Madrid, Spain.
authors.forename: Sylvia
authors.initials: S
authors.lastname: Ortega-Martínez
authors.affiliation: Molecular, Cellular and Developmental Neurobiology Department, Cajal Institute, CSIC, Madrid, Spain. Electronic address: jltrejo@cajal.csic.es.
authors.forename: José L
authors.initials: JL
authors.lastname: Trejo
mesh_terms: D000367:Age Factors; D000818:Animals; D001007:Anxiety; D001362:Avoidance Learning; D049109:Cell Proliferation; D018891:Dentate Gyrus / Q000187:drug effects* / Q000254:growth & development*; D003907:Dexamethasone / Q000494:pharmacology; D005260:Female; D005938:Glucocorticoids / Q000494:pharmacology*; D008297:Male; D008810:Mice, Inbred C57BL; D058953:Neural Stem Cells / Q000166:cytology / Q000187:drug effects* / Q000502:physiology*; D055495:Neurogenesis / Q000187:drug effects*; D013315:Stress, Psychological / Q000503:physiopathology; D013936:Thymidine / Q000031:analogs & derivatives / Q000494:pharmacology
chemical_list: D005938:Glucocorticoids; D003907:Dexamethasone; D013936:Thymidine
keywords: Adult hippocampal neurogenesis; Adult neural stem cells; Dexamethasone; Elevated Plus Maze; Passive Avoidance; Postnatal development