title: Electrochemical biosensor for DNA demethylase detection based on demethylation triggered endonuclease BstUI and Exonuclease III digestion.
abstract: Herein, an electrochemical biosensor was fabricated for DNA demethylase detection based on DNA demethylation triggered endonuclease BstUI and Exonuclease III digestion. After the double-strand DNA was demethylated, it can be further digested by BstUI and formed a blunt end at the electrode surface. Then, the remained fragment of DNA-DNA duplex was further cleaved by exonuclease III and led to increased electrochemical signal. Based on this detection strategy, the biosensor showed high sensitivity with low detection limit of 0.15ng/mL. Moreover, the developed method also presented high selectivity and acceptable reproducibility. This work provides a novel detection platform for DNA demethylase detection.
authors.affiliation: College of Chemistry and Material Science, Shandong Agricultural University, 271018 Taian, Shandong, PR China.
authors.forename: Huanshun
authors.initials: H
authors.lastname: Yin
authors.affiliation: College of Chemistry and Material Science, Shandong Agricultural University, 271018 Taian, Shandong, PR China.
authors.forename: Zhiqing
authors.initials: Z
authors.lastname: Yang
authors.affiliation: College of Chemistry and Material Science, Shandong Agricultural University, 271018 Taian, Shandong, PR China.
authors.forename: Bingchen
authors.initials: B
authors.lastname: Li
authors.affiliation: College of Chemistry and Material Science, Shandong Agricultural University, 271018 Taian, Shandong, PR China. Electronic address: zhyl@sdau.edu.cn.
authors.forename: Yunlei
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: College of Chemistry and Material Science, Shandong Agricultural University, 271018 Taian, Shandong, PR China. Electronic address: ashy@sdau.edu.cn.
authors.forename: Shiyun
authors.initials: S
authors.lastname: Ai
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D004247:DNA / Q000737:chemistry; D019175:DNA Methylation; D055664:Electrochemical Techniques / Q000379:methods*; D004720:Endonucleases / Q000737:chemistry; D005090:Exodeoxyribonucleases / Q000737:chemistry; D010090:Oxidoreductases, O-Demethylating / Q000302:isolation & purification*
chemical_list: D004247:DNA; D010090:Oxidoreductases, O-Demethylating; C117595:methyl-CpG DNA demethylase; D004720:Endonucleases; D005090:Exodeoxyribonucleases; C020133:exodeoxyribonuclease III
keywords: DNA demethylase; Electrochemical biosensor; Endonuclease BstUI; Exonuclease III