title: In vitro susceptibilities of caprine Mycoplasma agalactiae field isolates to six antimicrobial agents using the E test methodology.
abstract: The minimum inhibitory concentrations (MICs) of enrofloxacin, ciprofloxacin, spectinomycin, tetracycline, spiramycin and erythromycin against 30 caprine Greek isolates of Mycoplasma agalactiae were determined using E test methodology. The E test strips were placed on Eaton's agar medium without antimicrobials and phenol red. MICs were then read by determining where the growth inhibition zone intersected with the MIC scale on the strip. An MIC value of 8 µg/mL was considered as a guide to mycoplasma resistance. All isolates were sensitive to fluoroquinolones (MIC50, 0.19 g/mL; MIC90, 0.38 µg/mL; highest MIC, 0.5 µg/mL), spectinomycin (MIC50, 0.5 µg/mL; MIC90, 1 µg/mL; highest MIC, 1 µg/mL), and spiramycin (MIC50, 1 µg/mL; MIC90, 1.5 µg/mL; highest MIC, 2 µg/mL). Two strains exhibited resistance to tetracycline (MIC 32 µg/mL) but these were not found to carry any of the tet(M), tet(O), and tet(S) resistance genes. Finally all isolates expressed resistance to erythromycin (MIC50, 128 µg/mL; MIC90, >256 µg/mL).
authors.affiliation: Department of Microbiology and Infectious Diseases, Faculty of Veterinary Medicine, Aristotle University of Thessaloniki, University Campus, Thessaloniki 55134, Greece. Electronic address: georgefilious@vet.auth.gr.
authors.forename: George
authors.initials: G
authors.lastname: Filioussis
authors.affiliation: Department of Microbiology and Infectious Diseases, Faculty of Veterinary Medicine, Aristotle University of Thessaloniki, University Campus, Thessaloniki 55134, Greece.
authors.forename: Evanthia
authors.initials: E
authors.lastname: Petridou
authors.affiliation: Clinic of Farm Animals, Faculty of Veterinary Medicine, Aristotle University of Thessaloniki, University Campus, Thessaloniki 55134, Greece.
authors.forename: Nektarios D
authors.initials: ND
authors.lastname: Giadinis
authors.affiliation: Department of Microbiology and Infectious Diseases, Faculty of Veterinary Medicine, Aristotle University of Thessaloniki, University Campus, Thessaloniki 55134, Greece.
authors.forename: Spyridon K
authors.initials: SK
authors.lastname: Kritas
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology* / Q000627:therapeutic use; D024881:Drug Resistance, Bacterial; D005260:Female; D015511:Goat Diseases / Q000188:drug therapy / Q000382:microbiology*; D006041:Goats; D006115:Greece; D008826:Microbial Sensitivity Tests / Q000662:veterinary; D009175:Mycoplasma Infections / Q000188:drug therapy / Q000382:microbiology / Q000662:veterinary*; D045644:Mycoplasma agalactiae / Q000187:drug effects*
chemical_list: D000900:Anti-Bacterial Agents
keywords: E test; Goats; Greece; Minimal inhibitory concentration; Mycoplasma agalactiae