title: Potential lipid accumulation and growth characteristic of the green alga Chlorella with combination cultivation mode of nitrogen (N) and phosphorus (P).
abstract: This study aimed to evaluate the potential lipid accumulation of an oleaginous Chlorella protothecoides by combination cultivation mode of nitrogen (N) and phosphorus (P). Under co-deficiency of N and P, the largest lipid content (55.8%) was accomplished in C. protothecoides, which was higher than either sole P-deficiency (32.77%) or N-deficiency (52.5%), or co-repletion of N and P (control) (22.17%). However, the highest lipid productivity (224.14mg/L/day) with combination mode of N-deficiency and P-repletion represented 1.19-3.70-fold more than that of control, P-deficiency/limitation, and co-deficiency of N and P, respectively. This indicating N-deficiency plus P-repletion was a promising lipid trigger to motivate lipid accumulation in C. protothecoides cells. Further, difference gel electrophoresis (DIGE)-based proteomics was employed to reveal the molecular pathways associated with lipid biosynthesis. These results provide the foundation to develop engineering strategies targeting lipid productivity for industrial production of microalgae-based biodiesel.
authors.affiliation: School of Chemical Engineering, Xiangtan University, Xiangtan, China. Electronic address: yuqinli2004@xtu.edu.cn.
authors.forename: Yuqin
authors.initials: Y
authors.lastname: Li
authors.affiliation: School of Chemical Engineering, Xiangtan University, Xiangtan, China.
authors.forename: Fangxin
authors.initials: F
authors.lastname: Han
authors.affiliation: School of Chemical Engineering, Xiangtan University, Xiangtan, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Xu
authors.affiliation: School of Chemical Engineering, Xiangtan University, Xiangtan, China.
authors.forename: Jinxiu
authors.initials: J
authors.lastname: Mu
authors.affiliation: School of Chemical Engineering, Xiangtan University, Xiangtan, China.
authors.forename: Di
authors.initials: D
authors.lastname: Chen
authors.affiliation: School of Chemical Engineering, Xiangtan University, Xiangtan, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Feng
authors.affiliation: School of Chemical Engineering, Xiangtan University, Xiangtan, China.
authors.forename: Hongyan
authors.initials: H
authors.lastname: Zeng
mesh_terms: D020418:Algal Proteins / Q000378:metabolism; D018533:Biomass; D018929:Cell Culture Techniques / Q000379:methods*; D002708:Chlorella / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D005227:Fatty Acids / Q000378:metabolism; D050356:Lipid Metabolism / Q000187:drug effects*; D009584:Nitrogen / Q000494:pharmacology*; D010758:Phosphorus / Q000494:pharmacology*
chemical_list: D020418:Algal Proteins; D005227:Fatty Acids; D010758:Phosphorus; D009584:Nitrogen
keywords: Biomass; Chlorella protothecoides; Lipid accumulation; N-deficiency and P-repletion; Proteomics