title: Synergistic effect of curcumin on epigallocatechin gallate-induced anticancer action in PC3 prostate cancer cells.
abstract: Epigallocatechin gallate (EGCG) and curcumin are well known to naturally-occurring anticancer agents. The aim of this study was to verify the combined beneficial anticancer effects of curcumin and EGCG on PC3 prostate cancer cells, which are resistant to chemotherapy drugs and apoptosis inducers. EGCG showed weaker inhibitory effect on PC3 cell proliferation than two other prostate cancer cell lines, LNCaP and DU145. Co-treatment of curcumin improved antiproliferative effect of EGCG on PC3 cells. The protein expressions of p21 were significantly increased by the co-treatment of EGCG and curcumin, whereas it was not changed by the treatment with each individual compound. Moreover, treatments of EGCG and curcumin arrested both S and G2/M phases of PC3 cells. These results suggest that the enhanced inhibitory effect of EGCG on PC3 cell proliferation by curcumin was mediated by the synergic up-regulation of p21-induced growth arrest and followed cell growth arrest.
authors.affiliation: Department of Pathology, University of Ulsan College of Medicine, Gangneung Asan Hospital, Gangneung 25440, Korea.
authors.forename: Dae-Woon
authors.initials: DW
authors.lastname: Eom
authors.affiliation: Natural Products Research Institute, Korea Institute of Science and Technology, Gangneung 25451, Korea.
authors.forename: Ji Hwan
authors.initials: JH
authors.lastname: Lee
authors.affiliation: Natural Products Research Institute, Korea Institute of Science and Technology, Gangneung 25451, Korea.
authors.forename: Young-Joo
authors.initials: YJ
authors.lastname: Kim
authors.affiliation: College of Korean Medicine, Gachon University, Seongnam 13120, Korea.
authors.forename: Gwi Seo
authors.initials: GS
authors.lastname: Hwang
authors.affiliation: Natural Products Research Institute, Korea Institute of Science and Technology, Gangneung 25451, Korea.
authors.forename: Su-Nam
authors.initials: SN
authors.lastname: Kim
authors.affiliation: Department of Surgery, University of Ulsan College of Medicine, Gangneung Asan Hospital, Gangneung 25440, Korea.
authors.forename: Jin Ho
authors.initials: JH
authors.lastname: Kwak
authors.affiliation: Department of Internal Medicine, University of Ulsan College of Medicine, Gangneung Asan Hospital, Gangneung 25440, Korea.
authors.forename: Gab Jin
authors.initials: GJ
authors.lastname: Cheon
authors.affiliation: Natural Product Research Laboratory, School of Pharmacy, Sungkyunkwan University, Suwon 16419, Korea.
authors.forename: Ki Hyun
authors.initials: KH
authors.lastname: Kim
authors.affiliation: Department of Surgery, University of Ulsan College of Medicine, Gangneung Asan Hospital, Gangneung 25440, Korea.
authors.forename: Hyuk-Jai
authors.initials: HJ
authors.lastname: Jang
authors.affiliation: Natural Products Research Institute, Korea Institute of Science and Technology, Gangneung 25451, Korea.
authors.forename: Jungyeob
authors.initials: J
authors.lastname: Ham
authors.affiliation: College of Korean Medicine, Gachon University, Seongnam 13120, Korea.
authors.forename: Ki Sung
authors.initials: KS
authors.lastname: Kang
authors.affiliation: College of Korean Medicine, Gachon University, Seongnam 13120, Korea.
authors.forename: Noriko
authors.initials: N
authors.lastname: Yamabe
mesh_terms: D000971:Antineoplastic Combined Chemotherapy Protocols / Q000494:pharmacology*; D002392:Catechin / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000494:pharmacology; D059447:Cell Cycle Checkpoints / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D003474:Curcumin / Q000008:administration & dosage / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004357:Drug Synergism; D006801:Humans; D008297:Male; D011471:Prostatic Neoplasms / Q000188:drug therapy*; D015854:Up-Regulation / Q000187:drug effects
chemical_list: D002392:Catechin; C045651:epigallocatechin gallate; D003474:Curcumin