title: A new recombinant SsnA protein combined with aluminum hydroxide protects mouse against Streptococcus suis.
abstract: An experimental challenge in a mouse model was used to select the most effective adjuvant in a vaccine formulation with the surface-anchored DNA-nuclease (SsnA). We used a protocol based on clinical, histopathological, bacterial kinetics and immune response against S. suis serotype 2 in infected animals. The three adjuvants used, aluminum hydroxide (ALOH), incomplete Freund's adjuvant (FIA), oil-in-water adjuvant (OW) showed a protective effect against death by S. suis serotype 2 in this mouse model, although aluminum hydroxide revealed as the best option. Subsequently, in a second experimental assay, we showed that a recombinant SsnA protein combined with ALOH as adjuvant allowed a significant decrease of clinical and lesional findings in animals, faster reduction of the bacteria from organs and a highest humoral response against S. suis after 3 days post-infection. The results show that this combination (rSsnA+AlOH) could be a good vaccine formulation against S. suis, although further studies are necessary to evaluate their use for swine and human species.
authors.affiliation: Departamento de Sanidad Animal, Universidad de Córdoba, Campus de Excelencia Internacional CeiA3, 1407 Córdoba, Spain. Electronic address: v32gogal@hotmail.com.
authors.forename: Lidia
authors.initials: L
authors.lastname: Gómez-Gascón
authors.affiliation: Departamento de Sanidad Animal, Universidad de Córdoba, Campus de Excelencia Internacional CeiA3, 1407 Córdoba, Spain.
authors.forename: Fernando
authors.initials: F
authors.lastname: Cardoso-Toset
authors.affiliation: Departamento de Anatomía y Anatomía Patológica Comparada, Universidad de Córdoba, Campus de Excelencia Internacional CeiA3, 1407 Córdoba, Spain.
authors.forename: Paola Shyrley
authors.initials: PS
authors.lastname: Amarilla
authors.affiliation: Departamento de Sanidad Animal, Universidad de Córdoba, Campus de Excelencia Internacional CeiA3, 1407 Córdoba, Spain.
authors.forename: Carmen
authors.initials: C
authors.lastname: Tarradas
authors.affiliation: Departamento de Anatomía y Anatomía Patológica Comparada, Universidad de Córdoba, Campus de Excelencia Internacional CeiA3, 1407 Córdoba, Spain.
authors.forename: Librado
authors.initials: L
authors.lastname: Carrasco
authors.affiliation: Departamento de Bioquímica y Biología Molecular, Universidad de Córdoba, Campus de Excelencia Internacional CeiA3, 1407 Córdoba, Spain.
authors.forename: Alfonso
authors.initials: A
authors.lastname: Olaya-Abril
authors.affiliation: Departamento de Bioquímica y Biología Molecular, Universidad de Córdoba, Campus de Excelencia Internacional CeiA3, 1407 Córdoba, Spain.
authors.forename: Irene
authors.initials: I
authors.lastname: Jiménez-Munguía
authors.affiliation: Departamento de Bioquímica y Biología Molecular, Universidad de Córdoba, Campus de Excelencia Internacional CeiA3, 1407 Córdoba, Spain.
authors.forename: Manuel J
authors.initials: MJ
authors.lastname: Rodríguez-Ortega
authors.affiliation: Departamento de Sanidad Animal, Universidad de Córdoba, Campus de Excelencia Internacional CeiA3, 1407 Córdoba, Spain.
authors.forename: Inmaculada
authors.initials: I
authors.lastname: Luque
mesh_terms: D000276:Adjuvants, Immunologic / Q000008:administration & dosage*; D000536:Aluminum Hydroxide / Q000008:administration & dosage; D000818:Animals; D004195:Disease Models, Animal; D005260:Female; D005620:Freund's Adjuvant / Q000008:administration & dosage; D006801:Humans; D051379:Mice; D009821:Oils / Q000008:administration & dosage; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology; D013290:Streptococcal Infections / Q000517:prevention & control*; D022541:Streptococcal Vaccines / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D017011:Streptococcus suis / Q000235:genetics / Q000276:immunology*; D013552:Swine; D013553:Swine Diseases / Q000517:prevention & control; D016896:Treatment Outcome
chemical_list: D000276:Adjuvants, Immunologic; D009821:Oils; D011994:Recombinant Proteins; D022541:Streptococcal Vaccines; D000536:Aluminum Hydroxide; D005620:Freund's Adjuvant
keywords: Adjuvants; Mouse model; Recombinant SsnA-ALOH vaccine; Streptococcus suis serotype 2