title: Characterisation of bovine serum albumin-fucoidan conjugates prepared via the Maillard reaction.
abstract: Bovine serum albumin (BSA)-fucoidan conjugates were prepared by the Maillard reaction (60 °C and 79% relative humidity for 96 h), and were then identified by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and size-exclusion chromatography (SEC). Molecular characteristics of the BSA-fucoidan conjugates were investigated, using atomic force microscopy (AFM), dynamic light scattering (DLS), fluorescence spectroscopy, and circular dichroism spectroscopy. SDS-PAGE patterns provided evidence for the covalent bonding between BSA and fucoidan. SEC profiles showed that about 1.5-2.0 mol of fucoidan were covalently linked to 1 mol of BSA, resulting in high-molecular-weight compositions (conjugates). AFM images and DLS results indicated that most particles in the conjugates were nano-structured and more spherical than those of a regular BSA-fucoidan mixture. The fluorescence intensity and maximum emission wavelength of the conjugates together revealed that the BSA molecules had converted from an ordered conformation into a partially folded molten globule state.
authors.affiliation: Department of Food and Nutrition, College of Human Ecology, Hanyang University, Seoul 133-791, Republic of Korea.
authors.forename: Do-Yeong
authors.initials: DY
authors.lastname: Kim
authors.affiliation: Department of Food and Nutrition, College of Human Ecology, Hanyang University, Seoul 133-791, Republic of Korea. Electronic address: hime@hanyang.ac.kr.
authors.forename: Weon-Sun
authors.initials: WS
authors.lastname: Shin
mesh_terms: D000818:Animals; D002417:Cattle; D002850:Chromatography, Gel / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D015416:Maillard Reaction; D018625:Microscopy, Atomic Force / Q000379:methods; D008970:Molecular Weight; D011134:Polysaccharides / Q000737:chemistry*; D011487:Protein Conformation; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D013050:Spectrometry, Fluorescence / Q000379:methods
chemical_list: D011134:Polysaccharides; D012710:Serum Albumin, Bovine; C007789:fucoidan
keywords: Bovine serum albumin; Fucoidan; Maillard reaction; Molten globule state