title: Monoclonal antibody-based competitive enzyme-linked immunosorbent assay to detect antibodies to O:4 Salmonella in the sera of livestock and poultry.
abstract: Serotyping is an important element for surveillance of Salmonella. In this study, an anti-O:4 Salmonella monoclonal antibody-based competitive enzyme-linked immunosorbent assay that could identify Salmonella infection in cow, pig, horse, and chicken was developed. This detection system can therefore be useful for a wide range of animals and for humans.
authors.affiliation: Bacterial and Parasitic Disease Research Division, National Institute of Animal Health, NARO, Tsukuba, Ibaraki 305-0856, Japan.
authors.forename: Swarmistha Devi
authors.initials: SD
authors.lastname: Aribam
authors.affiliation: Bacterial and Parasitic Disease Research Division, National Institute of Animal Health, NARO, Tsukuba, Ibaraki 305-0856, Japan.
authors.forename: Yohsuke
authors.initials: Y
authors.lastname: Ogawa
authors.affiliation: Kitasato Institute for Life Sciences and Graduate School of Control Sciences, Kitasato University, 5-9-1 Shirokane, Minato-ku, Tokyo 108-8641, Japan.
authors.forename: Hidenori
authors.initials: H
authors.lastname: Matsui
authors.affiliation: Bacterial and Parasitic Disease Research Division, National Institute of Animal Health, NARO, Tsukuba, Ibaraki 305-0856, Japan.
authors.forename: Jiro
authors.initials: J
authors.lastname: Hirota
authors.affiliation: Laboratory of Zoonoses, Kitasato University School of Veterinary Medicine, Towada, Aomori 034-8628, Japan.
authors.forename: Masashi
authors.initials: M
authors.lastname: Okamura
authors.affiliation: Bacterial and Parasitic Disease Research Division, National Institute of Animal Health, NARO, Tsukuba, Ibaraki 305-0856, Japan.
authors.forename: Masato
authors.initials: M
authors.lastname: Akiba
authors.affiliation: Bacterial and Parasitic Disease Research Division, National Institute of Animal Health, NARO, Tsukuba, Ibaraki 305-0856, Japan.
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Shimoji
authors.affiliation: Bacterial and Parasitic Disease Research Division, National Institute of Animal Health, NARO, Tsukuba, Ibaraki 305-0856, Japan. Electronic address: egumaro@affrc.go.jp.
authors.forename: Masahiro
authors.initials: M
authors.lastname: Eguchi
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis*; D000911:Antibodies, Monoclonal / Q000032:analysis*; D002417:Cattle; D002645:Chickens; D004797:Enzyme-Linked Immunosorbent Assay / Q000295:instrumentation / Q000379:methods*; D006736:Horses; D058751:Livestock / Q000097:blood; D011201:Poultry Diseases / Q000097:blood* / Q000382:microbiology; D012475:Salmonella / Q000276:immunology / Q000302:isolation & purification*; D012481:Salmonella Infections, Animal / Q000097:blood* / Q000382:microbiology; D013552:Swine
chemical_list: D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal
keywords: Animal salmonellosis; Competitive ELISA; Group O:4 Salmonella; Monoclonal antibody