title: Preparation and anti-osteoporotic activities in vivo of phosphorylated peptides from Antarctic krill (Euphausia superba).
abstract: Antarctic krill (Euphausia superba) protein serves as a novel sustainable protein source for human. Krill protein isolate was phosphorylated by the dry-heating method with sodium pyrophosphate. Phosphorylated peptides from Antarctic krill (PP-AKP) were obtained from phosphorylated protein through tryptic hydrolysis. Two types of phosphate bonds were introduced by phosphorylation, i.e. PO and PO bonds. The anti-osteoporotic activities of PP-AKP at two doses (400 and 800mg/kg body weight) were investigated with an osteoporotic rat model, which was established with bilateral ovariectomy surgery. Different doses of PP-AKP were given intraperitoneal injections to rats once a day with alendronate as a positive control. Phosphorylated peptides from Antarctic krill dose-dependently preserved bone mineral density in osteoporotic rats by increasing the degree of bone mineralization. Both trabecular and cortical bone strength in osteoporotic rats was significantly improved with PP-AKP treatment. The mechanism by which PP-AKP augmented bone mineral density and bone strength was relation to the reduction in osteoclast-mediated bone remodeling, as was supported by the decrease in bone resorption markers. Phosphorylated peptides from Antarctic krill could be developed as functional food or nutritional supplements.
authors.affiliation: College of Food Science and Engineering, Ocean University of China, Qingdao, 266003, China.
authors.forename: Yanchao
authors.initials: Y
authors.lastname: Wang
authors.affiliation: College of Food Science and Engineering, Ocean University of China, Qingdao, 266003, China.
authors.forename: Shanshan
authors.initials: S
authors.lastname: Wang
authors.affiliation: College of Food Science and Engineering, Ocean University of China, Qingdao, 266003, China. Electronic address: wangyc1219@hotmail.com.
authors.forename: Jingfeng
authors.initials: J
authors.lastname: Wang
authors.affiliation: College of Food Science and Engineering, Ocean University of China, Qingdao, 266003, China. Electronic address: xuech@ouc.edu.cn.
authors.forename: Changhu
authors.initials: C
authors.lastname: Xue
authors.affiliation: College of Food Science and Engineering, Ocean University of China, Qingdao, 266003, China.
authors.forename: Yaoguang
authors.initials: Y
authors.lastname: Chang
authors.affiliation: College of Food Science and Engineering, Ocean University of China, Qingdao, 266003, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Xue
mesh_terms: D000818:Animals; D060829:Arthropod Proteins / Q000138:chemical synthesis / Q000494:pharmacology*; D015519:Bone Density / Q000187:drug effects; D050071:Bone Density Conservation Agents / Q000138:chemical synthesis / Q000494:pharmacology*; D016723:Bone Remodeling / Q000187:drug effects; D002118:Calcium / Q000097:blood; D004353:Drug Evaluation, Preclinical; D033201:Euphausiacea / Q000737:chemistry; D005260:Female; D010024:Osteoporosis / Q000097:blood / Q000188:drug therapy*; D010748:Phosphopeptides / Q000138:chemical synthesis / Q000494:pharmacology*; D010758:Phosphorus / Q000097:blood; D017207:Rats, Sprague-Dawley
chemical_list: D060829:Arthropod Proteins; D050071:Bone Density Conservation Agents; D010748:Phosphopeptides; D010758:Phosphorus; D002118:Calcium
keywords: Antarctic krill; Anti-osteoporotic activity; Bone; Phosphorylated peptides; Phosphorylation