title: Rapid identification of bacterial biofilms and biofilm wound models using a multichannel nanosensor.
abstract: Identification of infectious bacteria responsible for biofilm-associated infections is challenging due to the complex and heterogeneous biofilm matrix. To address this issue and minimize the impact of heterogeneity on biofilm identification, we developed a gold nanoparticle (AuNP)-based multichannel sensor to detect and identify biofilms based on their physicochemical properties. Our results showed that the sensor can discriminate six bacterial biofilms including two composed of uropathogenic bacteria. The capability of the sensor was further demonstrated through discrimination of biofilms in a mixed bacteria/mammalian cell in vitro wound model.
authors.affiliation: Department of Chemistry and ‡Biology Department, University of Massachusetts , 710 North Pleasant Street, Amherst, Massachusetts 01003, United States.
authors.forename: Xiaoning
authors.initials: X
authors.lastname: Li
authors.forename: Hao
authors.initials: H
authors.lastname: Kong
authors.forename: Rubul
authors.initials: R
authors.lastname: Mout
authors.forename: Krishnendu
authors.initials: K
authors.lastname: Saha
authors.forename: Daniel F
authors.initials: DF
authors.lastname: Moyano
authors.forename: Sandra M
authors.initials: SM
authors.lastname: Robinson
authors.forename: Subinoy
authors.initials: S
authors.lastname: Rana
authors.forename: Xinrong
authors.initials: X
authors.lastname: Zhang
authors.forename: Margaret A
authors.initials: MA
authors.lastname: Riley
authors.forename: Vincent M
authors.initials: VM
authors.lastname: Rotello
mesh_terms: D000818:Animals; D001419:Bacteria; D018441:Biofilms; D006046:Gold / Q000737:chemistry; D008164:Luminescent Proteins / Q000737:chemistry; D053768:Metal Nanoparticles / Q000737:chemistry; D051379:Mice; D041681:NIH 3T3 Cells; D036103:Nanotechnology / Q000295:instrumentation*; D013997:Time Factors
chemical_list: D008164:Luminescent Proteins; D006046:Gold
keywords: biofilms; biosensor design; fluorescent proteins; gold nanoparticles; multichannel sensor; uropathogen