title: Whole genomic analysis of porcine G10P[5] rotavirus strain P343 provides evidence for bovine-to-porcine interspecies transmission.
abstract: Porcine group A rotavirus (RVA) strain P343 (RVA/Pig-tc/THA/P343/1991/G10P[5]) was suggested to have VP7 and VP4 genes of bovine origin. In order to obtain precise information on the exact origin and evolution of this unusual porcine strain, the remaining nine genes (VP6, VP1-3, and NSP1-5) of strain P343 were sequenced and analyzed in the present study. On whole genomic analysis, strain P343 was found to have a bovine RVA-like genotype constellation (G10-P[5]-I2-R2-C2-M2-A3-N2-T6-E2-H3) different from those of typical porcine RVA strains. Furthermore, on phylogenetic analysis, each of the 11 genes of strain P343 appeared to be of bovine origin. Therefore, strain P343 was suggested to be a bovine RVA strain that was transmitted to pigs.
authors.affiliation: Department of Virology and Parasitology, Fujita Health University School of Medicine, Toyoake, Aichi 470-1192, Japan. Electronic address: satoshik@fujita-hu.ac.jp.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Komoto
authors.affiliation: Virus Research Institute, Department of Medical Sciences, National Institute of Health, Nonthaburi 11000, Thailand.
authors.forename: Yaowapa
authors.initials: Y
authors.lastname: Pongsuwanna
authors.affiliation: Department of Virology and Parasitology, Fujita Health University School of Medicine, Toyoake, Aichi 470-1192, Japan.
authors.forename: Tomihiko
authors.initials: T
authors.lastname: Ide
authors.affiliation: Department of Virology and Parasitology, Fujita Health University School of Medicine, Toyoake, Aichi 470-1192, Japan.
authors.forename: Mitsutaka
authors.initials: M
authors.lastname: Wakuda
authors.affiliation: Virus Research Institute, Department of Medical Sciences, National Institute of Health, Nonthaburi 11000, Thailand.
authors.forename: Ratigorn
authors.initials: R
authors.lastname: Guntapong
authors.affiliation: Department of Virology II, National Institute of Infectious Diseases, Musashi-Murayama, Tokyo 208-0011, Japan; Department of Environmental Parasitology, Tokyo Medical and Dental University, Bunkyo-ku, Tokyo 113-8510, Japan; Department of Electron Microscopy and Histopathology, Noguchi Memorial Institute for Medical Research, College of Health Sciences, University of Ghana, Legon LG581, Ghana.
authors.forename: Francis Ekow
authors.initials: FE
authors.lastname: Dennis
authors.affiliation: Department of Virology II, National Institute of Infectious Diseases, Musashi-Murayama, Tokyo 208-0011, Japan.
authors.forename: Kei
authors.initials: K
authors.lastname: Haga
authors.affiliation: Department of Virology II, National Institute of Infectious Diseases, Musashi-Murayama, Tokyo 208-0011, Japan.
authors.forename: Yoshiki
authors.initials: Y
authors.lastname: Fujii
authors.affiliation: Department of Virology II, National Institute of Infectious Diseases, Musashi-Murayama, Tokyo 208-0011, Japan.
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Katayama
authors.affiliation: Department of Virology and Parasitology, Fujita Health University School of Medicine, Toyoake, Aichi 470-1192, Japan.
authors.forename: Koki
authors.initials: K
authors.lastname: Taniguchi
mesh_terms: D000818:Animals; D001483:Base Sequence; D005075:Biological Evolution; D002417:Cattle; D002418:Cattle Diseases / Q000635:transmission* / Q000821:virology; D016679:Genome, Viral / Q000235:genetics*; D023281:Genomics; D005838:Genotype; D010802:Phylogeny; D012401:Rotavirus / Q000235:genetics* / Q000302:isolation & purification; D012400:Rotavirus Infections / Q000635:transmission / Q000662:veterinary* / Q000821:virology; D013552:Swine; D013553:Swine Diseases / Q000635:transmission* / Q000821:virology
keywords: Bovine-to-porcine interspecies transmission; Group A rotavirus; Whole genomic analysis