title: Reactive oxygen species contribute to TRAIL receptors upregulation; the mechanism for PH II-7 augmenting TRAIL induced apoptosis in leukemia cells.
abstract: Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) can selectively induce apoptosis in cancer cells and is verified to be effective in various cancers. However, a variety of cancer cells are found to be resistant to TRAIL and the mechanisms are largely unknown. Moreover, multidrug resistance to traditional chemotherapeutic agents still remains a tough problem in clinical practice. Fortunately, our previous work proved the ability of PH II-7 in overcoming MDR phenotype through reactive oxygen species production in K562 and its MDR counterpart K562/A02 cells. Additionally, we further explored its potential in augmenting TRAIL induced apoptosis in cancer cells with various tissue origins. Our results showed PH II-7 up-regulated DR4/DR5 expression and augment TRAIL cytotoxicity through reactive oxygen species production, which provide a solid foundation for TRAIL in combination with PH II-7 in future clinical application.
authors.affiliation: Department of Pharmacy, First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330006, PR China; State Key Laboratory of Experimental Hematology, Institute of Hematology & Hospital of Blood Diseases, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300020, PR China.
authors.forename: Hongwei
authors.initials: H
authors.lastname: Peng
authors.affiliation: State Key Laboratory of Experimental Hematology, Institute of Hematology & Hospital of Blood Diseases, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300020, PR China.
authors.forename: Xiangfei
authors.initials: X
authors.lastname: Yuan
authors.affiliation: Center for Experimental Medicine, First Affiliated Hospital of Nanchang University, Institute of Life Sciences, Nanchang University, Nanchang, Jiangxi 330006, PR China.
authors.forename: Shiwen
authors.initials: S
authors.lastname: Luo
authors.affiliation: Department of Hematology, First affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330006, PR China.
authors.forename: Fei
authors.initials: F
authors.lastname: Li
authors.affiliation: Department of Pharmacy, First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330006, PR China. Electronic address: wxh-hello@163.com.
authors.forename: Xiaohua
authors.initials: X
authors.lastname: Wei
authors.affiliation: Central Hospital of Karamay, Karamay, Xinjiang 834000, PR China. Electronic address: yezhou126@126.com.
authors.forename: Zhou
authors.initials: Z
authors.lastname: Ye
authors.affiliation: State Key Laboratory of Experimental Hematology, Institute of Hematology & Hospital of Blood Diseases, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300020, PR China. Electronic address: dsxiong@ihcams.ac.cn.
authors.forename: Dongsheng
authors.initials: D
authors.lastname: Xiong
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D019008:Drug Resistance, Neoplasm; D015973:Gene Expression Regulation, Leukemic / Q000187:drug effects; D006801:Humans; D007211:Indoles / Q000494:pharmacology*; D007938:Leukemia / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D018384:Oxidative Stress / Q000187:drug effects; D010446:Peptide Fragments / Q000819:agonists / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D034622:RNA Interference; D012333:RNA, Messenger / Q000378:metabolism; D034741:RNA, Small Interfering; D017382:Reactive Oxygen Species / Q000378:metabolism*; D053220:Receptors, TNF-Related Apoptosis-Inducing Ligand / Q000819:agonists* / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D012995:Solubility; D015854:Up-Regulation / Q000187:drug effects*
chemical_list: D000970:Antineoplastic Agents; D007211:Indoles; C569642:PHII-7 compound; D010446:Peptide Fragments; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D017382:Reactive Oxygen Species; D053220:Receptors, TNF-Related Apoptosis-Inducing Ligand; C105190:TNFRSF10A protein, human; C505639:TNFRSF10B protein, human
keywords: Apoptosis; Multidrug resistance; PH II-7; Reactive oxygen species; Tumor necrosis factor related apoptosis inducing ligand (TRAIL)