title: Comparison of two thin-film microextractions for the analysis of estrogens in aqueous tea extract and environmental water samples by high performance liquid chromatography-ultraviolet detection.
abstract: Two thin-film microextractions (TFME), octadecylsilane (ODS)-polyacrylonitrile (PAN)-TFME and polar enhanced phase (PEP)-PAN-TFME have been proposed for the analysis of bisphenol-A, diethylstilbestrol and 17β-estradiol in aqueous tea extract and environmental water samples followed by high performance liquid chromatography-ultraviolet detection. Both thin-films were prepared by spraying. The influencing factors including pH, extraction time, desorption solvent, desorption volume, desorption time, ion strength and reusability were investigated. Under the optimal conditions, the two TFME methods are similar in terms of the analytical performance evaluated by standard addition method. The limits of detection for three estrogens in environmental water and aqueous tea extract matrix ranged from 1.3 to 1.6 and 2.8 to 7.1 ng mL(-1) by the two TFME methods, respectively. Both approaches were applied for the analysis of analytes in real aqueous tea extract and environmental water samples, presenting satisfactory recoveries ranged from 87.3% to 109.4% for the spiked samples.
authors.affiliation: Department of Pharmaceutical Analysis, School of Pharmacy, Tongji Medical College, Huazhong University of Science and Technology, PR China.
authors.forename: Pei-Shan
authors.initials: PS
authors.lastname: Cai
authors.affiliation: Department of Pharmaceutical Analysis, School of Pharmacy, Tongji Medical College, Huazhong University of Science and Technology, PR China.
authors.forename: Dan
authors.initials: D
authors.lastname: Li
authors.affiliation: Department of Pharmaceutical Analysis, School of Pharmacy, Tongji Medical College, Huazhong University of Science and Technology, PR China.
authors.forename: Jing
authors.initials: J
authors.lastname: Chen
authors.affiliation: Department of Pharmaceutical Analysis, School of Pharmacy, Tongji Medical College, Huazhong University of Science and Technology, PR China. Electronic address: cmxiong2002@hust.edu.cn.
authors.forename: Chao-Mei
authors.initials: CM
authors.lastname: Xiong
authors.affiliation: Department of Pharmaceutical Analysis, School of Pharmacy, Tongji Medical College, Huazhong University of Science and Technology, PR China.
authors.forename: Jin-Lan
authors.initials: JL
authors.lastname: Ruan
mesh_terms: D001559:Benzhydryl Compounds / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D004054:Diethylstilbestrol / Q000032:analysis; D004958:Estradiol / Q000032:analysis; D004967:Estrogens / Q000032:analysis*; D005504:Food Analysis; D005618:Fresh Water / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D060106:Lakes; D009994:Osmolar Concentration; D010636:Phenols / Q000032:analysis; D010936:Plant Extracts / Q000032:analysis*; D015203:Reproducibility of Results; D045483:Rivers; D012997:Solvents; D013662:Tea / Q000737:chemistry*; D000098826:Bisphenol A Compounds
chemical_list: D001559:Benzhydryl Compounds; D004054:Diethylstilbestrol; D004958:Estradiol; D004967:Estrogens; D010636:Phenols; D010936:Plant Extracts; D012997:Solvents; D013662:Tea; C006780:bisphenol A; D000098826:Bisphenol A Compounds
keywords: Estrogens; Octadecylsilane sorbent; Polar enhanced phase sorbent; Polyacrylonitrile; Thin-film microextraction