title: TRPM7 is required for ovarian cancer cell growth, migration and invasion.
abstract: Our previous study demonstrated that the melastatin-related transient receptor potential channel 7 (TRPM7) was highly expressed in ovarian carcinomas and its overexpression was significantly associated with poor prognosis in ovarian cancer patients. However, the function of TRPM7 in ovarian cancer is mostly unknown. In this study, we examined the roles of TRPM7 in ovarian cancer cell proliferation, migration and invasion. We found that short hairpin RNA interference-mediated silence of TRPM7 significantly inhibited cell proliferation, colony formation, migration and invasion in multiple ovarian cancer cell lines. Mechanistic investigation revealed that silence of TRPM7 decreased phosphorylation levels of Akt, Src and p38 and increased filamentous actin and focal adhesion number in ovarian cancer cells. Thus, our results suggest that TRPM7 is required for proliferation, migration and invasion of ovarian cancer cells through regulating multiple signaling transduction pathways and the formation of focal adhesions.
authors.affiliation: The Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University, Changsha 410013, PR China.
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.affiliation: The Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University, Changsha 410013, PR China.
authors.forename: Qian-Jin
authors.initials: QJ
authors.lastname: Liao
authors.affiliation: Department of Obstetrics and Gynaecology, Xiangya Hospital, Central South University, Changsha 410078, PR China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: The Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University, Changsha 410013, PR China.
authors.forename: Hui
authors.initials: H
authors.lastname: Zhou
authors.affiliation: The Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University, Changsha 410013, PR China.
authors.forename: Chen-Hui
authors.initials: CH
authors.lastname: Luo
authors.affiliation: The Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University, Changsha 410013, PR China.
authors.forename: Jie
authors.initials: J
authors.lastname: Tang
authors.affiliation: The Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University, Changsha 410013, PR China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Wang
authors.affiliation: The Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University, Changsha 410013, PR China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Tang
authors.affiliation: The Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University, Changsha 410013, PR China.
authors.forename: Min
authors.initials: M
authors.lastname: Zhao
authors.affiliation: The Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University, Changsha 410013, PR China.
authors.forename: Xue-Heng
authors.initials: XH
authors.lastname: Zhao
authors.affiliation: Department of Basic Medical Science, Yongzhou Vocational Technical College, Yong Zhou 425100, PR China.
authors.forename: Qiong-Yu
authors.initials: QY
authors.lastname: Zhang
authors.affiliation: Department of Histology and Embryology, School of Basic Medical Sciences, Central South University, Changsha 410013, PR China; Institute of Clinical Pharmacology, Central South University, Changsha 410018, PR China. Electronic address: lingxiaocsu@126.com.
authors.forename: Ling
authors.initials: L
authors.lastname: Xiao
mesh_terms: D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D005260:Female; D057809:HEK293 Cells; D006801:Humans; D010051:Ovarian Neoplasms / Q000378:metabolism* / Q000473:pathology*; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism*; D015398:Signal Transduction; D050053:TRPM Cation Channels / Q000378:metabolism*
chemical_list: D050053:TRPM Cation Channels; D017346:Protein Serine-Threonine Kinases; C486583:TRPM7 protein, human
keywords: Cell proliferation; Ion channel; Metastasis; Ovarian cancer; TRPM7