title: Neolignan inhibitors of antigen-induced degranulation in RBL-2H3 cells from the needles of Pinus thunbergii.
abstract: The ethanolic extract of the needles of Pinus thunbergii was found to suppress antigen mediated degranulation of rat basophilic leukemia (RBL-2H3) cells. A new neolignan glycoside, named pinusthunbergiside A (1), as well as six known neolignan glycosides (2-7) were isolated from the ethanolic extract using bioassay-guided fractionation. Their structures were elucidated by a combination of 1D and 2D NMR, HRESI-MS, and circular dichroism (CD) data. Compounds 2-7 were found for the first time in this plant. The inhibitory effects of isolated constituents on the release of β-hexosaminidase from RBL-2H3 cells were examined, and compounds 2, 3, 5, and 6 were found to show the inhibitory activity with IC₅₀ values ranging between 52.3 and 75.3 μM.
authors.affiliation: Gyeonggi Institute of Science & Technology Promotion Natural Products Research Institute, Suwon 443-766, Republic of Korea.
authors.forename: Seong Su
authors.initials: SS
authors.lastname: Hong
authors.affiliation: Gyeonggi Institute of Science & Technology Promotion Natural Products Research Institute, Suwon 443-766, Republic of Korea; College of Pharmacy, Dankook University, Cheonan 330-714, Republic of Korea.
authors.forename: Wonsik
authors.initials: W
authors.lastname: Jeong
authors.affiliation: Gyeonggi Institute of Science & Technology Promotion Natural Products Research Institute, Suwon 443-766, Republic of Korea.
authors.forename: Jin Kyu
authors.initials: JK
authors.lastname: Kim
authors.affiliation: Gyeonggi Institute of Science & Technology Promotion Natural Products Research Institute, Suwon 443-766, Republic of Korea.
authors.forename: Jin Gwan
authors.initials: JG
authors.lastname: Kwon
authors.affiliation: Gyeonggi Institute of Science & Technology Promotion Natural Products Research Institute, Suwon 443-766, Republic of Korea.
authors.forename: Jae Yeon
authors.initials: JY
authors.lastname: Lee
authors.affiliation: Gyeonggi Institute of Science & Technology Promotion Natural Products Research Institute, Suwon 443-766, Republic of Korea.
authors.forename: Eun-Kyung
authors.initials: EK
authors.lastname: Ahn
authors.affiliation: Department of Biology, Duke University, Durham, NC 27708, USA.
authors.forename: Junho
authors.initials: J
authors.lastname: Oh
authors.affiliation: College of Pharmacy, Dankook University, Cheonan 330-714, Republic of Korea.
authors.forename: Dong-Wan
authors.initials: DW
authors.lastname: Seo
authors.affiliation: Gyeonggi Institute of Science & Technology Promotion Natural Products Research Institute, Suwon 443-766, Republic of Korea; College of Pharmacy, Dankook University, Cheonan 330-714, Republic of Korea. Electronic address: jsoh@dankook.ac.kr.
authors.forename: Joa Sub
authors.initials: JS
authors.lastname: Oh
mesh_terms: D000818:Animals; D015550:Cell Degranulation / Q000187:drug effects*; D045744:Cell Line, Tumor; D006027:Glycosides / Q000737:chemistry*; D020128:Inhibitory Concentration 50; D017705:Lignans / Q000737:chemistry* / Q000302:isolation & purification; D015394:Molecular Structure; D028223:Pinus / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry; D051381:Rats; D001619:beta-N-Acetylhexosaminidases / Q000037:antagonists & inhibitors*
chemical_list: D006027:Glycosides; D017705:Lignans; C000597712:pinusthunbergiside A; D001619:beta-N-Acetylhexosaminidases
keywords: Neolignan glycoside; Pinus thunbergii; Pinusthunbergiside A; β-hexosaminidase