title: Constituent analysis and quality control of Lamiophlomis rotata by LC-TOF/MS and HPLC-UV.
abstract: In the present study, chemical profile analysis for Lamiophlomis rotata, a classic Tibetan folk medicine, was illustrated by an ultra-high performance liquid chromatography quadrupole time-of-flight mass spectrometry (LC-TOF/MS) method, which provided evidence for the certain identification of the main constituents, including iridoids, flavonoids and phenylethanoid glycosides. Among these compounds, nine of them were regarded as marker compounds for the quantitative evaluation of L. rotata, using a simple and reliable method by HPLC with ultraviolet, in combination of chromatographic fingerprint analysis. Separation was achieved on a Waters SunFire C18 analytical column with linear gradient elution of acetonitrile and 0.1% formic acid aqueous solution. The validated method was successfully applied to evaluate twelve batches of L. rotata. Assay results showed that nine compounds did not vary significantly from the aerial parts and the whole plant for each batch, and was consistent with the fingerprint analysis, which confirmed the medicine parts alteration in Chinese Pharmacopoeia from the perspective of chemical components.
authors.affiliation: Department of Pharmacy, Changzheng Hospital, Second Military Medical University, 415 Fengyang Road, Shanghai 200003, PR China.
authors.forename: Mingping
authors.initials: M
authors.lastname: La
authors.affiliation: Department of Pharmacy, Changzheng Hospital, Second Military Medical University, 415 Fengyang Road, Shanghai 200003, PR China.
authors.forename: Feng
authors.initials: F
authors.lastname: Zhang
authors.affiliation: Department of Pharmacy, Changzheng Hospital, Second Military Medical University, 415 Fengyang Road, Shanghai 200003, PR China.
authors.forename: Shouhong
authors.initials: S
authors.lastname: Gao
authors.affiliation: Department of Pharmacy, Changzheng Hospital, Second Military Medical University, 415 Fengyang Road, Shanghai 200003, PR China.
authors.forename: Xiaowei
authors.initials: X
authors.lastname: Liu
authors.affiliation: Department of Pharmacy, Changzheng Hospital, Second Military Medical University, 415 Fengyang Road, Shanghai 200003, PR China.
authors.forename: Zhijun
authors.initials: Z
authors.lastname: Wu
authors.affiliation: Department of Pharmacy, Changzheng Hospital, Second Military Medical University, 415 Fengyang Road, Shanghai 200003, PR China.
authors.forename: Lianna
authors.initials: L
authors.lastname: Sun
authors.affiliation: Department of Pharmacy, Changzheng Hospital, Second Military Medical University, 415 Fengyang Road, Shanghai 200003, PR China.
authors.forename: Xia
authors.initials: X
authors.lastname: Tao
authors.affiliation: Department of Pharmacy, Changzheng Hospital, Second Military Medical University, 415 Fengyang Road, Shanghai 200003, PR China. Electronic address: chenwanshengsmmu@aliyun.com.
authors.forename: Wansheng
authors.initials: W
authors.lastname: Chen
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002853:Chromatography, Liquid / Q000379:methods; D004365:Drugs, Chinese Herbal / Q000032:analysis* / Q000592:standards*; D011786:Quality Control; D013056:Spectrophotometry, Ultraviolet / Q000379:methods; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D004365:Drugs, Chinese Herbal
keywords: Fingerprint analysis; HPLC-UV; Iridoids; LC-TOF/MS; Lamiophlomis rotata