title: Standardized isolation and culture of murine liver sinusoidal endothelial cells.
abstract: Isolation of murine liver sinusoidal endothelial cells (LSECs) is an exacting and finicky procedure. After exhaustive standardization, we were able to devise an easily reproducible protocol which produced consistent results. Moreover, we scripted a protocol which clarifies even the smallest of steps, following which isolation of LSECs is made significantly easier. Using the standardized LSEC isolation protocol herein, we demonstrated that the bacterial toxin pyocyanin (from Pseudomonas aeruginosa) induced a significant dose-dependent reduction in LSEC porosity, this being preventable by the enzyme catalase, but not by the enzyme superoxide dismutase.
authors.affiliation: Department of Medicine, St. George Clinical School, University of New South Wales, Sydney, New South Wales, Australia.
authors.forename: Rajkumar
authors.initials: R
authors.lastname: Cheluvappa
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods* / Q000592:standards; D002469:Cell Separation / Q000379:methods* / Q000592:standards; D042783:Endothelial Cells / Q000166:cytology / Q000378:metabolism; D006801:Humans; D008099:Liver / Q000166:cytology / Q000378:metabolism; D051379:Mice; D011550:Pseudomonas aeruginosa / Q000737:chemistry; D011710:Pyocyanine / Q000737:chemistry
chemical_list: D011710:Pyocyanine
keywords: LSECs; Pseudomonas aeruginosa; cell culture; hepatocytes; liver sinusoidal endothelial cells; portal; pyocyanin