title: Smart human serum albumin-indocyanine green nanoparticles generated by programmed assembly for dual-modal imaging-guided cancer synergistic phototherapy.
abstract: Phototherapy, including photodynamic therapy (PDT) and photothermal therapy (PTT), is a light-activated local treatment modality that is under intensive preclinical and clinical investigations for cancer. To enhance the treatment efficiency of phototherapy and reduce the light-associated side effects, it is highly desirable to improve drug accumulation and precision guided phototherapy for efficient conversion of the absorbed light energy to reactive oxygen species (ROS) and local hyperthermia. In the present study, a programmed assembly strategy was developed for the preparation of human serum albumin (HSA)-indocyanine green (ICG) nanoparticles (HSA-ICG NPs) by intermolecular disulfide conjugations. This study indicated that HSA-ICG NPs had a high accumulation with tumor-to-normal tissue ratio of 36.12±5.12 at 24 h and a long-term retention with more than 7 days in 4T1 tumor-bearing mice, where the tumor and its margin, normal tissue were clearly identified via ICG-based in vivo near-infrared (NIR) fluorescence and photoacoustic dual-modal imaging and spectrum-resolved technology. Meanwhile, HSA-ICG NPs efficiently induced ROS and local hyperthermia simultaneously for synergetic PDT/PTT treatments under a single NIR laser irradiation. After an intravenous injection of HSA-ICG NPs followed by imaging-guided precision phototherapy (808 nm, 0.8 W/cm2 for 5 min), the tumor was completely suppressed, no tumor recurrence and treatments-induced toxicity were observed. The results suggest that HSA-ICG NPs generated by programmed assembly as smart theranostic nanoplatforms are highly potential for imaging-guided cancer phototherapy with PDT/PTT synergistic effects.
authors.affiliation: Guangdong Key Laboratory of Nanomedicine, CAS Key Laboratory of Health Informatics, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences , Shenzhen 518055, PR China.
authors.forename: Zonghai
authors.initials: Z
authors.lastname: Sheng
authors.forename: Dehong
authors.initials: D
authors.lastname: Hu
authors.forename: Mingbin
authors.initials: M
authors.lastname: Zheng
authors.forename: Pengfei
authors.initials: P
authors.lastname: Zhao
authors.forename: Huilong
authors.initials: H
authors.lastname: Liu
authors.forename: Duyang
authors.initials: D
authors.lastname: Gao
authors.forename: Ping
authors.initials: P
authors.lastname: Gong
authors.forename: Guanhui
authors.initials: G
authors.lastname: Gao
authors.forename: Pengfei
authors.initials: P
authors.lastname: Zhang
authors.forename: Yifan
authors.initials: Y
authors.lastname: Ma
authors.forename: Lintao
authors.initials: L
authors.lastname: Cai
mesh_terms: D000818:Animals; D001692:Biological Transport; D002460:Cell Line; D006801:Humans; D007208:Indocyanine Green / Q000737:chemistry*; D008297:Male; D008325:Mammary Neoplasms, Experimental / Q000175:diagnosis* / Q000628:therapy*; D051379:Mice; D057054:Molecular Imaging; D053758:Nanoparticles / Q000737:chemistry / Q000627:therapeutic use*; D036103:Nanotechnology; D010789:Phototherapy / Q000009:adverse effects / Q000379:methods*; D012449:Safety; D012709:Serum Albumin / Q000737:chemistry*
chemical_list: D012709:Serum Albumin; D007208:Indocyanine Green
keywords: indocyanine green; photoacoustic imaging; photodynamic therapy; photothermal therapy; synergistic effect; theranostics