title: An enzyme-free and amplified colorimetric detection strategy via target-aptamer binding triggered catalyzed hairpin assembly.
abstract: Here we introduce an enzyme-free and colorimetric detection strategy for small molecule adenosine. The approach is based on the adenosine-aptamer binding triggered liberation of an initiator strand that consecutively catalyzes DNA hairpins hybridized from singles to couples. These couples induce gold nanoparticles assembled via crosslinking, which could be visualized by a color change.
authors.affiliation: State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Key Laboratory for Bio-Nanotechnology and Molecular Engineering of Hunan Province, Hunan University, Changsha 410082, China. kmwang@hnu.edu.cn.
authors.forename: Ke
authors.initials: K
authors.lastname: Quan
authors.forename: Jin
authors.initials: J
authors.lastname: Huang
authors.forename: Xiaohai
authors.initials: X
authors.lastname: Yang
authors.forename: Yanjing
authors.initials: Y
authors.lastname: Yang
authors.forename: Le
authors.initials: L
authors.lastname: Ying
authors.forename: He
authors.initials: H
authors.lastname: Wang
authors.forename: Yong
authors.initials: Y
authors.lastname: He
authors.forename: Kemin
authors.initials: K
authors.lastname: Wang
mesh_terms: D000241:Adenosine / Q000032:analysis* / Q000378:metabolism; D052157:Aptamers, Nucleotide / Q000737:chemistry / Q000378:metabolism*; D055162:Biocatalysis; D015374:Biosensing Techniques / Q000379:methods*; D003124:Colorimetry / Q000379:methods*; D006046:Gold / Q000737:chemistry; D055029:Inverted Repeat Sequences; D053768:Metal Nanoparticles / Q000737:chemistry; D009693:Nucleic Acid Hybridization
chemical_list: D052157:Aptamers, Nucleotide; D006046:Gold; D000241:Adenosine