title: Suppression of production of baboon endogenous virus by dominant negative mutants of cellular factors involved in multivesicular body sorting pathway.
abstract: Baboon endogenous virus (BaEV) is an infectious endogenous gammaretrovirus isolated from a baboon placenta. BaEV-related sequences have been identified in both Old World monkeys and African apes, but not in humans or Asian apes. Recently, it was reported that BaEV-like particles were produced from Vero cells derived from African green monkeys by chemical induction, and thus BaEV-like particles may contaminate biological products manufactured using Vero cells. In this study, we constructed an infectious molecular clone of BaEV strain M7. We found two putative L-domain motifs, PPPY and PSAP, in the pp15 region of Gag. To examine the function of the L-domain motifs, we conducted virus budding assay using L-domain motif mutants. We revealed that the PPPY motif, but not the PSAP motif, plays a major role as the L-domain in BaEV budding. We also demonstrated that Vps4A/B are involved in BaEV budding. These data suggest that BaEV Gag recruits the cellular endosomal sorting complex required for transport (ESCRT) machinery through the interaction of the PPPY L-domain with cellular factors. These data will be useful for controlling contamination of BaEV-like particles in biological products in the future.
authors.affiliation: Laboratory of Signal Transduction, Department of Cell Biology, Institute for Virus Research, Kyoto University, 53 Shogoin-Kawaharacho, Sakyo-ku, Kyoto 606-8507, Japan.
authors.forename: Rokusuke
authors.initials: R
authors.lastname: Yoshikawa
authors.affiliation: Laboratory of Signal Transduction, Department of Cell Biology, Institute for Virus Research, Kyoto University, 53 Shogoin-Kawaharacho, Sakyo-ku, Kyoto 606-8507, Japan.
authors.forename: Rie Nakaoka
authors.initials: RN
authors.lastname: Miyaho
authors.affiliation: Laboratory of Signal Transduction, Department of Cell Biology, Institute for Virus Research, Kyoto University, 53 Shogoin-Kawaharacho, Sakyo-ku, Kyoto 606-8507, Japan.
authors.forename: Akira
authors.initials: A
authors.lastname: Hashimoto
authors.affiliation: Fifth Biology Section for Microbiology, First Department of Forensic Science, National Research Institute of Police Science, 6-3-1 Kashiwanoha, Kashiwai, Chiba 277-0882, Japan.
authors.forename: Masumi
authors.initials: M
authors.lastname: Abe
authors.affiliation: Department of Emerging Infectious Disease, Institute of Tropical Medicine, Nagasaki University, Nagasaki 852-8523, Japan.
authors.forename: Jiro
authors.initials: J
authors.lastname: Yasuda
authors.affiliation: Laboratory of Signal Transduction, Department of Cell Biology, Institute for Virus Research, Kyoto University, 53 Shogoin-Kawaharacho, Sakyo-ku, Kyoto 606-8507, Japan. Electronic address: takavet@goo.jp.
authors.forename: Takayuki
authors.initials: T
authors.lastname: Miyazawa
mesh_terms: D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D000818:Animals; D002460:Cell Line; D002522:Chlorocebus aethiops; D056827:Endosomal Sorting Complexes Required for Transport / Q000378:metabolism*; D017988:Gammaretrovirus / Q000502:physiology*; D015683:Gene Products, gag / Q000737:chemistry / Q000235:genetics; D016679:Genome, Viral; D054884:Host-Pathogen Interactions; D006801:Humans; D008969:Molecular Sequence Data; D057146:Multivesicular Bodies / Q000378:metabolism* / Q000821:virology; D009154:Mutation; D054730:Protein Interaction Domains and Motifs; D012367:RNA, Viral; D014709:Vero Cells; D057074:Virus Release; D014779:Virus Replication
chemical_list: D056827:Endosomal Sorting Complexes Required for Transport; D015683:Gene Products, gag; D012367:RNA, Viral
keywords: Baboon; Baboon endogenous virus; Budding; Host factors