title: Surface profiles of live colorectal cancer cells and tumor infiltrating lymphocytes from surgical samples correspond to prognostic categories.
abstract: Extensive surface profiles of colorectal cancer (CRC) cells and tumor infiltrating lymphocytes (TIL) have been obtained from 45 surgical resection samples. Live cells were captured on an antibody microarray and stained with fluorescently-labeled antibodies. Minimal panels of 11 CRC antigens (CD13, CD24, CD26, CD49d, CD138, CD166, CA-125, CA19-9, EGFR, Galectin-4 and HLA-DR) and 11 T-cell antigens (CD10, CD11b, CD11c, CD25, CD31, CD95, CD151, CD181, Galectin-4, CA19-9, TSP-1) provide signatures for relapse and survival. Hierarchical clustering of profiles from CRC cells and TIL identified groups of patients for survival, systemic relapse and death. The groups from CRC and TIL profiles for systemic relapse showed 79.2% concordance, enabling prediction of relapse after surgery. The results demonstrate communication between CRC cells and TIL.
authors.affiliation: School of Molecular Bioscience, University of Sydney, Sydney, NSW 2006, Australia.
authors.forename: Jerry
authors.initials: J
authors.lastname: Zhou
authors.affiliation: School of Molecular Bioscience, University of Sydney, Sydney, NSW 2006, Australia.
authors.forename: Larissa
authors.initials: L
authors.lastname: Belov
authors.affiliation: Discipline of Surgery, Sydney Clinical School, University of Sydney, Sydney, NSW 2006, Australia; Department of Colorectal Surgery, Concord Hospital, Concord West, NSW 2139, Australia.
authors.forename: Pierre
authors.initials: P
authors.lastname: Chapuis
authors.affiliation: Department of Anatomical Pathology, Concord Hospital, Concord West, NSW 2139, Australia; Discipline of Pathology, Concord Clinical School, University of Sydney, Sydney, NSW 2006, Australia.
authors.forename: Charles
authors.initials: C
authors.lastname: Chan
authors.affiliation: School of Mathematics and Statistics, University of Sydney, Sydney, NSW 2006, Australia.
authors.forename: Nicola
authors.initials: N
authors.lastname: Armstrong
authors.affiliation: School of Molecular Bioscience, University of Sydney, Sydney, NSW 2006, Australia.
authors.forename: Kimberley L
authors.initials: KL
authors.lastname: Kaufman
authors.affiliation: Discipline of Surgery, Sydney Clinical School, University of Sydney, Sydney, NSW 2006, Australia; Discipline of Surgery, Royal Prince Alfred Hospital, Camperdown, NSW 2050, Australia.
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Solomon
authors.affiliation: Bill Walsh Cancer Research Laboratories, Royal North Shore Hospital, St Leonards, NSW 2065, Australia.
authors.forename: Stephen J
authors.initials: SJ
authors.lastname: Clarke
authors.affiliation: School of Molecular Bioscience, University of Sydney, Sydney, NSW 2006, Australia. Electronic address: richard.christopherson@sydney.edu.au.
authors.forename: Richard I
authors.initials: RI
authors.lastname: Christopherson
mesh_terms: D000368:Aged; D015703:Antigens, CD / Q000276:immunology; D015179:Colorectal Neoplasms / Q000276:immunology* / Q000473:pathology*; D018984:Epitopes, T-Lymphocyte / Q000276:immunology; D005260:Female; D006684:HLA-DR Antigens / Q000276:immunology; D006801:Humans; D016246:Lymphocytes, Tumor-Infiltrating / Q000276:immunology* / Q000473:pathology*; D008297:Male; D009364:Neoplasm Recurrence, Local / Q000276:immunology / Q000473:pathology; D011379:Prognosis
chemical_list: D015703:Antigens, CD; D018984:Epitopes, T-Lymphocyte; D006684:HLA-DR Antigens
keywords: Antibody microarray; Biomarkers; Colorectal cancer; Disease signature; Tumor infiltrating T-lymphocytes