title: Quality traits analysis and protein profiling of field pea (Pisum sativum) germplasm from Himalayan region.
abstract: The grain and flour characteristics of different field pea (FP) accessions were evaluated. Accessions with higher grain weight had less compact structure with a greater proportion of large-sized starch granules. Accessions with higher protein content had lower starch content, blue value and λ(max) whereas accessions with higher amylose showed higher resistant starch (RS) and final viscosity and lower rapidly digestible starch (RDS). Ca, Zn, K and Fe content vary significantly amongst different accessions and creamish green and white seeds accessions showed higher Fe and Zn content. Yellow coloured accessions (1.36-3.71%) showed lower antioxidant activity as compared to brownish and green coloured accessions (4.06-9.30%). Out of 21 major polypeptides observed (9-100 kDa), 11 showed differential trypsin inhibitory activity (TIA) under non-reducing conditions. Polypeptides of 68, 46, 33 and 22 kDa showed prominent TIA.
authors.affiliation: Department of Food Science and Technology, Guru Nanak Dev University, Amritsar 143005, Punjab, India.
authors.forename: Shagun
authors.initials: S
authors.lastname: Sharma
authors.affiliation: Department of Food Science and Technology, Guru Nanak Dev University, Amritsar 143005, Punjab, India. Electronic address: narpinders@yahoo.com.
authors.forename: Narpinder
authors.initials: N
authors.lastname: Singh
authors.affiliation: Department of Food Science and Technology, Guru Nanak Dev University, Amritsar 143005, Punjab, India.
authors.forename: Amardeep Singh
authors.initials: AS
authors.lastname: Virdi
authors.affiliation: National Bureau of Plant Genetic Resources (NBPGR), Regional Station, Phagli, Shimla 171004, HP, India.
authors.forename: Jai Chand
authors.initials: JC
authors.lastname: Rana
mesh_terms: D000688:Amylose / Q000032:analysis; D000975:Antioxidants / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D005433:Flour / Q000032:analysis; D008670:Metals / Q000032:analysis; D018532:Pisum sativum / Q000737:chemistry / Q000378:metabolism*; D010636:Phenols / Q000737:chemistry; D010940:Plant Proteins / Q000378:metabolism*; D012639:Seeds / Q000737:chemistry / Q000378:metabolism; D013213:Starch / Q000737:chemistry / Q000302:isolation & purification; D014361:Trypsin Inhibitors / Q000032:analysis; D014783:Viscosity
chemical_list: D000975:Antioxidants; D008670:Metals; D010636:Phenols; D010940:Plant Proteins; D014361:Trypsin Inhibitors; D013213:Starch; D000688:Amylose
keywords: Antioxidant activity; Minerals; Resistant starch; SDS–PAGE; SEM; Trypsin inhibitor