title: Building the Connectivity Map of epigenetics: chromatin profiling by quantitative targeted mass spectrometry.
abstract: Epigenetic control of genome function is an important regulatory mechanism in diverse processes such as lineage commitment and environmental sensing, and in disease etiologies ranging from neuropsychiatric disorders to cancer. Here we report a robust, high-throughput targeted, quantitative mass spectrometry (MS) method to rapidly profile modifications of the core histones of chromatin that compose the epigenetic landscape, enabling comparisons among cells with differing genetic backgrounds, genomic perturbations, and drug treatments.
authors.affiliation: The Broad Institute of MIT and Harvard University, 415 Main St., Cambridge, MA 02142, United States.
authors.forename: Amanda L
authors.initials: AL
authors.lastname: Creech
authors.affiliation: The Broad Institute of MIT and Harvard University, 415 Main St., Cambridge, MA 02142, United States.
authors.forename: Jordan E
authors.initials: JE
authors.lastname: Taylor
authors.affiliation: Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, United States.
authors.forename: Verena K
authors.initials: VK
authors.lastname: Maier
authors.affiliation: The Broad Institute of MIT and Harvard University, 415 Main St., Cambridge, MA 02142, United States.
authors.forename: Xiaoyun
authors.initials: X
authors.lastname: Wu
authors.affiliation: The Broad Institute of MIT and Harvard University, 415 Main St., Cambridge, MA 02142, United States.
authors.forename: Caitlin M
authors.initials: CM
authors.lastname: Feeney
authors.affiliation: The Broad Institute of MIT and Harvard University, 415 Main St., Cambridge, MA 02142, United States.
authors.forename: Namrata D
authors.initials: ND
authors.lastname: Udeshi
authors.affiliation: The Broad Institute of MIT and Harvard University, 415 Main St., Cambridge, MA 02142, United States.
authors.forename: Sally E
authors.initials: SE
authors.lastname: Peach
authors.affiliation: The Broad Institute of MIT and Harvard University, 415 Main St., Cambridge, MA 02142, United States.
authors.forename: Jesse S
authors.initials: JS
authors.lastname: Boehm
authors.affiliation: Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, United States; Howard Hughes Medical Institute, Harvard Medical School, Boston, MA 02114, United States.
authors.forename: Jeannie T
authors.initials: JT
authors.lastname: Lee
authors.affiliation: The Broad Institute of MIT and Harvard University, 415 Main St., Cambridge, MA 02142, United States.
authors.forename: Steven A
authors.initials: SA
authors.lastname: Carr
authors.affiliation: The Broad Institute of MIT and Harvard University, 415 Main St., Cambridge, MA 02142, United States. Electronic address: jjaffe@broadinstitute.org.
authors.forename: Jacob D
authors.initials: JD
authors.lastname: Jaffe
mesh_terms: D002843:Chromatin / Q000737:chemistry*; D057890:Epigenomics / Q000379:methods*; D023281:Genomics; D006657:Histones / Q000378:metabolism; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D011499:Protein Processing, Post-Translational; D040901:Proteomics
chemical_list: D002843:Chromatin; D006657:Histones
keywords: Chromatin; Epigenetics; Histones; Mass spectrometry; Proteomics; SILAC