title: Assessment of integrated process based on hydrothermal and alkaline treatments for enzymatic saccharification of sweet sorghum stems.
abstract: In this study, sweet sorghum stem was subjected to hydrothermal pretreatment (HTP) and alkaline post-treatment to enhance its saccharification ratio by reducing its recalcitrance. The results showed that the HTP (110-210°C, 0.5-2.0h) significantly degraded hemicelluloses, and the pretreatment at the temperature higher than 190°C led to the partial degradation of the cellulose. As compared to the sole HTP, the integrated process removed most of lignin and hemicelluloses, which incurred a higher cellulose saccharification ratio. Under an optimum condition evaluated (HTP at 170°C for 0.5h and subsequent 2% NaOH treatment), 77.5% saccharification ratio was achieved, which was 1.8, 2.0 and 5.5 times as compared to the only HTP pretreated substrates, alkaline treated substrates alone and the raw material without pretreatment, respectively. Clearly, the integrated process can be considered as a promising approach to achieve an efficient conversion of lignocellulose to fermentable glucose.
authors.affiliation: Beijing Key Laboratory of Lignocellulosic Chemistry, Beijing Forestry University, Beijing, China.
authors.forename: Shao-Long
authors.initials: SL
authors.lastname: Sun
authors.affiliation: Beijing Key Laboratory of Lignocellulosic Chemistry, Beijing Forestry University, Beijing, China.
authors.forename: Shao-Ni
authors.initials: SN
authors.lastname: Sun
authors.affiliation: Beijing Key Laboratory of Lignocellulosic Chemistry, Beijing Forestry University, Beijing, China.
authors.forename: Jia-Long
authors.initials: JL
authors.lastname: Wen
authors.affiliation: Beijing Key Laboratory of Lignocellulosic Chemistry, Beijing Forestry University, Beijing, China.
authors.forename: Xue-Ming
authors.initials: XM
authors.lastname: Zhang
authors.affiliation: Beijing Key Laboratory of Lignocellulosic Chemistry, Beijing Forestry University, Beijing, China. Electronic address: pengfeng0523@yahoo.com.cn.
authors.forename: Feng
authors.initials: F
authors.lastname: Peng
authors.affiliation: Beijing Key Laboratory of Lignocellulosic Chemistry, Beijing Forestry University, Beijing, China.
authors.forename: Run-Cang
authors.initials: RC
authors.lastname: Sun
mesh_terms: D000863:Antacids / Q000494:pharmacology*; D019149:Bioreactors; D050260:Carbohydrate Metabolism / Q000187:drug effects; D002480:Cellulase / Q000378:metabolism*; D002482:Cellulose / Q000378:metabolism; D005285:Fermentation / Q000187:drug effects; D005947:Glucose / Q000378:metabolism; D006868:Hydrolysis / Q000187:drug effects; D008031:Lignin / Q000378:metabolism; D018547:Plant Stems / Q000378:metabolism*; D011134:Polysaccharides / Q000378:metabolism; D045868:Sorghum / Q000378:metabolism*; D013696:Temperature; D014867:Water / Q000494:pharmacology
chemical_list: D000863:Antacids; D011134:Polysaccharides; D014867:Water; C036909:lignocellulose; C007916:hemicellulose; D002482:Cellulose; D008031:Lignin; D002480:Cellulase; D005947:Glucose
keywords: Alkaline treatment; Enzymatic saccharification; Hydrothermal pretreatment; Sweet sorghum stem