title: Effects of stomatal development on stomatal conductance and on stomatal limitation of photosynthesis in Syringa oblata and Euonymus japonicus Thunb.
abstract: During leaf development, the increase in stomatal conductance cannot meet photosynthetic demand for CO2, thus leading to stomatal limitation of photosynthesis (Ls). Considering the crucial influences of stomatal development on stomatal conductance, we speculated whether stomatal development limits photosynthesis to some extent. To test this hypothesis, stomatal development, stomatal conductance and photosynthesis were carefully studied in both Syringa oblata (normal greening species) and Euonymus japonicus Thunb (delayed greening species). Our results show that the size of stomata increased gradually with leaf expansion, resulting in increased stomatal conductance up to the time of full leaf expansion. During this process, photosynthesis also increased steadily. Compared to that in S. oblata, the development of chloroplasts in E. japonicus Thunb was obviously delayed, leading to a delay in the improvement of photosynthetic capacity. Further analysis revealed that before full leaf expansion, stomatal limitation increased rapidly in both S. oblata and E. japonicus Thunb; after full leaf expansion, stomatal limitation continually increased in E. japonicus Thunb. Accordingly, we suggested that the enhancement of photosynthetic capacity is the main factor leading to stomatal limitation during leaf development but that stomatal development can alleviate stomatal limitation with the increase of photosynthesis by controlling gas exchange.
authors.affiliation: College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083, China; Key Laboratory of Plant Resources, Institute of Botany, The Chinese Academy of Sciences, Beijing 100093, China.
authors.forename: Bing-Jie
authors.initials: BJ
authors.lastname: Wu
authors.affiliation: Division of Plant Science, Research School of Biology, Australian National University, Canberra, ACT 0200, Australia.
authors.forename: Wah Soon
authors.initials: WS
authors.lastname: Chow
authors.affiliation: College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083, China. Electronic address: yjliubio@163.com.
authors.forename: Yu-Jun
authors.initials: YJ
authors.lastname: Liu
authors.affiliation: Key Laboratory of Plant Resources, Institute of Botany, The Chinese Academy of Sciences, Beijing 100093, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Shi
authors.affiliation: Key Laboratory of Plant Resources, Institute of Botany, The Chinese Academy of Sciences, Beijing 100093, China. Electronic address: jcdao@ibcas.ac.cn.
authors.forename: Chuang-Dao
authors.initials: CD
authors.lastname: Jiang
mesh_terms: D002245:Carbon Dioxide / Q000378:metabolism; D002734:Chlorophyll / Q000378:metabolism; D002736:Chloroplasts / Q000378:metabolism; D002940:Circadian Rhythm / Q000502:physiology; D029756:Euonymus / Q000502:physiology*; D009929:Organ Size; D017785:Photons; D010788:Photosynthesis; D054046:Plant Stomata / Q000033:anatomy & histology / Q000254:growth & development* / Q000502:physiology*; D031662:Syringa / Q000502:physiology*; D013997:Time Factors
chemical_list: D002734:Chlorophyll; D002245:Carbon Dioxide
keywords: Euonymus japonicus Thunb; Leaf development; Photosynthesis; Stomatal development; Syringa oblata