title: Structural and biochemical characterizations of an intramolecular tandem coiled coil protein.
abstract: Coiled coil has served as an excellent model system for studying protein folding and developing protein-based biomaterials. Most designed coiled coils function as oligomers, namely intermolecular coiled coils. However, less is known about structural and biochemical behavior of intramolecular coiled coils where coiled coil domains are covalently linked in one polypeptide. Here we prepare a protein which harbors three coiled coil domains with two short linkers, termed intramolecular tandem coiled coil (ITCC) and characterize its structural and biochemical behavior in solution. ITCC consists of three coiled coil domains whose sequences are derived from Coil-Ser and its domain swapped dimer. Modifications include positioning E (Glu) residue at "e" and K (Lys) at "g" positions throughout heptad repeats to enhance ionic interaction among its constituent coiled coil domains. Molecular modeling of ITCC suggests a compact triple helical bundle structure with the second and the third coiled coil domains forming a canonical coiled coil. ITCC exists as a mixture of monomeric and dimeric species in solution. Small-angle X-ray scattering reveals ellipsoidal molecular envelopes for both dimeric and monomeric ITCC in solution. The theoretically modeled structures of ITCC dock well into the envelopes of both species. Higher ionic strength shifts the equilibrium into monomer with apparently more compact structure while secondary structure remains unchanged. Taken together, our results suggest that our designed ITCC is predominantly monomeric structure through the enhanced ionic interactions, and its conformation is affected by the concentration of ionic species in the buffer.
authors.affiliation: Department of Biological Sciences, Sungkyunwan University, Suwon, Republic of Korea.
authors.forename: Donghyuk
authors.initials: D
authors.lastname: Shin
authors.affiliation: Department of Biological Sciences, Sungkyunwan University, Suwon, Republic of Korea.
authors.forename: Gwanho
authors.initials: G
authors.lastname: Kim
authors.affiliation: Department of Biological Sciences, Sungkyunwan University, Suwon, Republic of Korea.
authors.forename: Gyuhee
authors.initials: G
authors.lastname: Kim
authors.affiliation: Department of Chemistry, Sungkyunwan University, Suwon, Republic of Korea.
authors.forename: Xu
authors.initials: X
authors.lastname: Zheng
authors.affiliation: Department of Chemistry, Sungkyunwan University, Suwon, Republic of Korea.
authors.forename: Yang-Gyun
authors.initials: YG
authors.lastname: Kim
authors.affiliation: Department of Biological Sciences, Sungkyunwan University, Suwon, Republic of Korea. Electronic address: sangholee@skku.edu.
authors.forename: Sangho
authors.initials: S
authors.lastname: Lee
mesh_terms: D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000378:metabolism; D007477:Ions; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry; D016133:Polymerase Chain Reaction; D017510:Protein Folding; D055503:Protein Multimerization; D020836:Protein Structure, Quaternary; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D011506:Proteins / Q000737:chemistry*; D012542:Scattering, Radiation; D014965:X-Rays
chemical_list: D007477:Ions; D010455:Peptides; D011506:Proteins
keywords: Coiled coil; Ionic strength; Small-angle X-ray scattering; Solution structure