title: First report of bovine viral diarrhoea virus-2 infection in cattle in Poland.
abstract: This report describes the first identification in Poland of bovine viral diarrhoea virus (BVDV)-2 in a dairy herd where severe clinical disease with losses of young animals was observed. The virus was readily cultivated in cell culture and a phylogenetic analysis of the nucleotide sequences and secondary structures of the viral genomic 5' untranslated region confirmed virus identity. The economic impact of the infection was significant compared to the previously prevalent BVDV-1 infections confirming that this genotype of BVDV can cause severe sickness in affected herds. The use of BVDV-1 vaccine did not prevent the infection with the BVDV-2 genotype.
authors.affiliation: National Veterinary Research Institute, Partyzantów 57, Puławy 24-100, Poland. Electronic address: ppolak@piwet.pulawy.pl.
authors.forename: Mirosław P
authors.initials: MP
authors.lastname: Polak
authors.affiliation: National Veterinary Research Institute, Partyzantów 57, Puławy 24-100, Poland.
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Kuta
authors.affiliation: Veterinary Clinic, Łopusze 4, Drohiczyn 17-312, Poland.
authors.forename: Wiesław
authors.initials: W
authors.lastname: Rybałtowski
authors.affiliation: National Veterinary Research Institute, Partyzantów 57, Puławy 24-100, Poland.
authors.forename: Jerzy
authors.initials: J
authors.lastname: Rola
authors.affiliation: National Veterinary Research Institute, Partyzantów 57, Puławy 24-100, Poland.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Larska
authors.affiliation: National Veterinary Research Institute, Partyzantów 57, Puławy 24-100, Poland.
authors.forename: Jan F
authors.initials: JF
authors.lastname: Zmudziński
mesh_terms: D000818:Animals; D002417:Cattle; D030242:Diarrhea Virus 2, Bovine Viral / Q000235:genetics / Q000302:isolation & purification*; D005260:Female; D030243:Hemorrhagic Syndrome, Bovine / Q000453:epidemiology* / Q000821:virology; D008969:Molecular Sequence Data; D010802:Phylogeny; D011044:Poland; D060888:Real-Time Polymerase Chain Reaction / Q000662:veterinary; D017422:Sequence Analysis, DNA / Q000662:veterinary
keywords: BVDV; Bovine viral diarrhoea virus; Genotyping; Pestivirus; Phylogeny