title: A simple route to [11C]N-Me labeling of aminosuberic acid for proof of feasibility imaging of the x(C)⁻ transporter.
abstract: Oxidative stress has been implicated in a variety of conditions, including cancer, heart failure, diabetes, neurodegeneration and other diseases. A potential biomarker for oxidative stress is the cystine/glutamate transporter, system x(C)(-). L-Aminosuberic acid (L-ASu) has been identified as a system x(C)(-) substrate. Here we report a facile method for [(11)C]N-Me labeling of L-ASu, automation of the radiochemical process, and preliminary PET imaging with EL4 tumor bearing mice. The results demonstrate uptake in the tumor above background, warranting further studies on the use of radiolabeled analogs of L-ASu as a PET imaging agent for system x(C)(-).
authors.affiliation: Nuclear Medicine Division, TRIUMF, Vancouver V6T 2A3, Canada.
authors.forename: Hua
authors.initials: H
authors.lastname: Yang
authors.affiliation: Nuclear Medicine Division, TRIUMF, Vancouver V6T 2A3, Canada.
authors.forename: Qing
authors.initials: Q
authors.lastname: Miao
authors.affiliation: Diagnostics and Biomedical Technologies, GE Global Research, Niskayuna, NY 12309, USA.
authors.forename: Bruce F
authors.initials: BF
authors.lastname: Johnson
authors.affiliation: Diagnostics and Biomedical Technologies, GE Global Research, Niskayuna, NY 12309, USA.
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Rishel
authors.affiliation: Department of Physics and Astronomy, University of British Columbia, Vancouver V6T 1Z1, Canada.
authors.forename: Vesna
authors.initials: V
authors.lastname: Sossi
authors.affiliation: Department of Physics and Astronomy, University of British Columbia, Vancouver V6T 1Z1, Canada.
authors.forename: Katherine
authors.initials: K
authors.lastname: Dinelle
authors.affiliation: Centre of Excellence for Functional Cancer Imaging, British Columbia Cancer Agency, Vancouver V5Z 4E6, Canada; Department of Radiology, Faculty of Medicine, University of British Columbia, Vancouver V5C 1M9, Canada.
authors.forename: François
authors.initials: F
authors.lastname: Bénard
authors.affiliation: Experimental Therapeutics, British Columbia Cancer Agency, Vancouver V5Z 4E6, Canada; Division of Pharmaceutics and Biopharmaceutics, Faculty of Pharmacy, University of British Columbia, Vancouver V6T 1Z3, Canada.
authors.forename: Donald T
authors.initials: DT
authors.lastname: Yapp
authors.affiliation: Diagnostics and Biomedical Technologies, GE Global Research, Niskayuna, NY 12309, USA.
authors.forename: Jack M
authors.initials: JM
authors.lastname: Webster
authors.affiliation: Nuclear Medicine Division, TRIUMF, Vancouver V6T 2A3, Canada; Department of Radiology, Faculty of Medicine, University of British Columbia, Vancouver V5C 1M9, Canada. Electronic address: pschaffer@triumf.ca.
authors.forename: Paul
authors.initials: P
authors.lastname: Schaffer
mesh_terms: D027182:Amino Acid Transport System y+ / Q000378:metabolism; D000600:Amino Acids, Dicarboxylic / Q000378:metabolism*; D000818:Animals; D002478:Cells, Cultured; D003952:Diagnostic Imaging / Q000379:methods*; D026901:Membrane Transport Proteins; D051379:Mice; D015394:Molecular Structure; D009369:Neoplasms / Q000378:metabolism*; D018384:Oxidative Stress; D015854:Up-Regulation
chemical_list: D027182:Amino Acid Transport System y+; D000600:Amino Acids, Dicarboxylic; D026901:Membrane Transport Proteins; C019146:2-aminosuberic acid
keywords: C-11; Cystine transporter; N-methylation; Oxidative stress; Radiolabelling