title: Portable and sensitive quantitative detection of DNA based on personal glucose meters and isothermal circular strand-displacement polymerization reaction.
abstract: A portable and sensitive quantitative DNA detection method based on personal glucose meters and isothermal circular strand-displacement polymerization reaction was developed. The target DNA triggered target recycling process, which opened capture DNA. The released target then found another capture DNA to trigger another polymerization cycle, which was repeated for many rounds, resulting in the multiplication of the DNA-invertase conjugation on the surface of Streptavidin-MNBs. The DNA-invertase was used to catalyze the hydrolysis of sucrose into glucose for PGM readout. There was a liner relationship between the signal of PGM and the concentration of target DNA in the range of 5.0 to 1000 fM, which is lower than some DNA detection method. In addition, the method exhibited excellent sequence selectivity and there was almost no effect of biological complex to the detection performance, which suggested our method can be successfully applied to DNA detection in real biological samples.
authors.affiliation: HKUST Fok Ying Tung Graduate School, GuangZhou, GuangDong 511458, PR China. Electronic address: xuxuetao201405@163.com.
authors.forename: Xue-tao
authors.initials: XT
authors.lastname: Xu
authors.affiliation: Faculté des Sciences et techniques, Université du Maine Avenue Olivier Messiaen, 72085 Le Mans Cedex 9, France.
authors.forename: Kai-yi
authors.initials: KY
authors.lastname: Liang
authors.affiliation: South China University of Technology, PR China.
authors.forename: Jia-ying
authors.initials: JY
authors.lastname: Zeng
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation*; D015190:Blood Glucose Self-Monitoring / Q000295:instrumentation*; D004247:DNA / Q000032:analysis* / Q000235:genetics*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D017743:Equipment Reuse; D008904:Miniaturization; D021141:Nucleic Acid Amplification Techniques / Q000295:instrumentation*; D016133:Polymerase Chain Reaction / Q000295:instrumentation*; D017422:Sequence Analysis, DNA / Q000295:instrumentation*
chemical_list: D004247:DNA
keywords: DNA detection; Personal glucose meters; Portable; Sensitive; Target recycling process