title: Global profiling of protein lipidation using chemical proteomic technologies.
abstract: Protein lipidation is unique amongst post-translational modifications (PTMs) in enabling direct interaction with cell membranes, and is found in every form of life. Lipidation is important in normal function and in disease, but its intricate interplay with disease context presents a challenging for drug development. Global whole-proteome profiling of protein lipidation lies beyond the range of standard methods, but is well-suited to metabolic tagging with small 'clickable' chemical reporters that do not disrupt metabolism and function; chemoselective reactions are then used to add multifunctional labels exclusively to tagged-lipidated proteins. This chemical proteomic technology has opened up the first quantitative whole-proteome studies of the known major classes of protein lipidation, and the first insights into their full scope in vivo.
authors.affiliation: Department of Chemistry, Imperial College London, Exhibition Road, London SW7 2AZ, UK. Electronic address: e.tate@imperial.ac.uk.
authors.forename: Edward W
authors.initials: EW
authors.lastname: Tate
authors.affiliation: Department of Chemistry, Imperial College London, Exhibition Road, London SW7 2AZ, UK.
authors.forename: Karunakaran A
authors.initials: KA
authors.lastname: Kalesh
authors.affiliation: Department of Chemistry, Imperial College London, Exhibition Road, London SW7 2AZ, UK.
authors.forename: Thomas
authors.initials: T
authors.lastname: Lanyon-Hogg
authors.affiliation: Department of Chemistry, Imperial College London, Exhibition Road, London SW7 2AZ, UK.
authors.forename: Elisabeth M
authors.initials: EM
authors.lastname: Storck
authors.affiliation: The Rockefeller University, 1230 York Avenue, New York, USA.
authors.forename: Emmanuelle
authors.initials: E
authors.lastname: Thinon
mesh_terms: D000215:Acylation; D000818:Animals; D002784:Cholesterol / Q000032:analysis / Q000378:metabolism; D017261:Glycosylphosphatidylinositols / Q000032:analysis / Q000378:metabolism; D006801:Humans; D050356:Lipid Metabolism; D008055:Lipids / Q000032:analysis; D011499:Protein Processing, Post-Translational; D011506:Proteins / Q000737:chemistry* / Q000378:metabolism; D020543:Proteome / Q000737:chemistry / Q000378:metabolism; D040901:Proteomics / Q000379:methods
chemical_list: D017261:Glycosylphosphatidylinositols; D008055:Lipids; D011506:Proteins; D020543:Proteome; D002784:Cholesterol