title: Preparative separation of quaternary ammonium alkaloids from Coptis chinensis Franch by pH-zone-refining counter-current chromatography.
abstract: pH-zone-refining counter-current chromatography was successfully applied to the preparative separation of five quaternary ammonium alkaloids from the crude extract of Coptis chinensis Franch. The separation was performed with a two-phase solvent system composed of chloroform-methanol-water (4:3:3, v/v), where the upper aqueous stationary phase was added with 60mM of hydrochloric acid and the lower organic mobile phase with 5mM of triethylamine. From 1.0 g of crude extract, 5.4 mg of columbamine at 96.6% purity, 6.1mg of jateorhizine at 98.8% purity, 58.3mg of coptisine at 99.5% purity, 25.6 mg of palmatine at 98.4% purity and 503.9 mg of berberine at 99.5% purity were obtained. The purities of the isolated alkaloids were analyzed by HPLC and the chemical structures were identified by electrospray ionization-mass spectrometry and (1)H NMR.
authors.affiliation: Shandong Analysis and Test Center, Shandong Academy of Sciences, 19 Keyuan Street, Jinan, Shandong 250014, China; College of Pharmacy, Shandong University of Traditional Chinese Medicine, 4655 Daxue Street, Jinan, Shandong 250355, China.
authors.forename: Changlei
authors.initials: C
authors.lastname: Sun
authors.affiliation: College of Pharmacy, Shandong University of Traditional Chinese Medicine, 4655 Daxue Street, Jinan, Shandong 250355, China.
authors.forename: Jia
authors.initials: J
authors.lastname: Li
authors.affiliation: Shandong Analysis and Test Center, Shandong Academy of Sciences, 19 Keyuan Street, Jinan, Shandong 250014, China. Electronic address: wangx@sdas.org.
authors.forename: Xiao
authors.initials: X
authors.lastname: Wang
authors.affiliation: Shandong Analysis and Test Center, Shandong Academy of Sciences, 19 Keyuan Street, Jinan, Shandong 250014, China.
authors.forename: Wenjuan
authors.initials: W
authors.lastname: Duan
authors.affiliation: Shandong Analysis and Test Center, Shandong Academy of Sciences, 19 Keyuan Street, Jinan, Shandong 250014, China.
authors.forename: Tianyou
authors.initials: T
authors.lastname: Zhang
authors.affiliation: Laboratory of Bioseparation Technology, Biochemistry and Biophysics Center, National Heart, Lung, and Blood Institute, National Institutes of Health, 10 Center Drive, Bethesda, MD 20892, USA. Electronic address: itoy@nhlbi.nih.gov.
authors.forename: Yoichiro
authors.initials: Y
authors.lastname: Ito
mesh_terms: D000470:Alkaloids / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D039323:Coptis / Q000737:chemistry*; D003377:Countercurrent Distribution / Q000379:methods; D006863:Hydrogen-Ion Concentration; D009682:Magnetic Resonance Spectroscopy; D010936:Plant Extracts / Q000737:chemistry; D000644:Quaternary Ammonium Compounds / Q000302:isolation & purification*; D012997:Solvents / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D000470:Alkaloids; D010936:Plant Extracts; D000644:Quaternary Ammonium Compounds; D012997:Solvents
keywords: Coptis chinensis Franch; Quaternary ammonium alkaloids; pH-zone-refining counter-current chromatography