title: Changes and overlapping distribution in the expression of CB1/OX1-GPCRs in rat hippocampus by kainic acid-induced status epilepticus.
abstract: Status epilepticus (SE) is a life-threatening neurological disorder. It is important to discover new drugs to control SE without the development of pharmacoresistance. Focus on the cannabinoid receptor and cannabinoid-related compounds might be a good option. Cannabinoid receptor 1 (CB1) and orexin receptor 1 (OX1) both belong to the GPCR superfamily and display "cross-talk" interactions, however, there has been no study of the effect of OX1/CB1 in epilepsy. Therefore, we investigated the potential long-term effects of SE on CB1 and OX1 expression in rat hippocampus, aiming to elucidate whether they are involved in the causative mechanism of epilepsy and whether they might form a heterodimer. In this study, SE was induced with kainic acid, and results of immunohistochemistry and RT-PCR both showed that the expression of CB1 in the hippocampus increased after SE and was significantly higher compared to controls especially 1 week post-SE. However we did not find any significant difference in the expression of OX1 between the SE group and the controls at any time. Under immunofluorescence staining, we observed an overlapping distribution of CB1 and OX1 in the hippocampus. The increased expression of CB1 in the hippocampus indicates that CB1 may play an important role in the underlying mechanism of SE, but the effect of OX1 was not obvious. The overlapping distribution of CB1 and OX1 in the hippocampus indicates that they may form a heterodimer to exert their effect in epilepsy.
authors.affiliation: Department of Neurology, Chinese PLA General Hospital, No. 28, Fuxing Road, Beijing 100853, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Zhu
authors.affiliation: Department of Neurology, Chinese PLA General Hospital, No. 28, Fuxing Road, Beijing 100853, China. Electronic address: bjxqwang@yahoo.com.cn.
authors.forename: Xiang-Qing
authors.initials: XQ
authors.lastname: Wang
authors.affiliation: Department of Neurology, Chinese PLA General Hospital, No. 28, Fuxing Road, Beijing 100853, China.
authors.forename: Ya-Nan
authors.initials: YN
authors.lastname: Chen
authors.affiliation: Department of Pharmacology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences & Peking Union Medical College, No. 5, Dong Dan San Tiao, Beijing 100005, China.
authors.forename: Nan
authors.initials: N
authors.lastname: Yang
authors.affiliation: Department of Neurology, Chinese PLA General Hospital, No. 28, Fuxing Road, Beijing 100853, China. Electronic address: langsy@263.net.
authors.forename: Sen-Yang
authors.initials: SY
authors.lastname: Lang
authors.affiliation: Department of Pharmacology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences & Peking Union Medical College, No. 5, Dong Dan San Tiao, Beijing 100005, China.
authors.forename: Ping-Ping
authors.initials: PP
authors.lastname: Zuo
authors.affiliation: Department of Neurology, Chinese PLA General Hospital, No. 28, Fuxing Road, Beijing 100853, China.
authors.forename: Jia-Tang
authors.initials: JT
authors.lastname: Zhang
authors.affiliation: Research and Technology Service Center, 302 Hospital of PLA, No. 100, Middle Section of West 4th Ring Road, Beijing 100039, China.
authors.forename: Rui-Sheng
authors.initials: RS
authors.lastname: Li
mesh_terms: D000818:Animals; D034961:Antigens, Nuclear / Q000378:metabolism; D002462:Cell Membrane / Q000378:metabolism; D004195:Disease Models, Animal; D018450:Disease Progression; D005455:Fluorescent Antibody Technique; D006624:Hippocampus / Q000378:metabolism* / Q000473:pathology; D007150:Immunohistochemistry; D007211:Indoles; D007608:Kainic Acid; D008297:Male; D009419:Nerve Tissue Proteins / Q000378:metabolism; D064446:Orexin Receptors / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D017208:Rats, Wistar; D060888:Real-Time Polymerase Chain Reaction; D043884:Receptor, Cannabinoid, CB1 / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013226:Status Epilepticus / Q000378:metabolism* / Q000473:pathology
chemical_list: D034961:Antigens, Nuclear; C543264:Cnr1 protein, rat; C578627:Hcrtr1 protein, rat; D007211:Indoles; D009419:Nerve Tissue Proteins; D064446:Orexin Receptors; D012333:RNA, Messenger; C546339:Rbfox3 protein, rat; D043884:Receptor, Cannabinoid, CB1; C007293:DAPI; D007608:Kainic Acid
keywords: Cannabinoid receptor 1; G-protein coupled receptors; Kainic acid; Orexin receptor 1; Status epilepticus