title: Antagonistic activity of dietary allicin against deltamethrin-induced oxidative damage in freshwater Nile tilapia; Oreochromis niloticus.
abstract: Allicin, the main biologically active component of garlic clove extracts, has been evaluated for its' efficacy in preventing deltamethrin-induced oxidative damage in Nile tilapia; Oreochromis niloticus. Fish were fed on 2 different doses of 0.5 g and 1 g of allicin/kg diet for 28 days. Serum aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), cholesterol, urea, uric acid, creatinine, total protein, albumin and globulin were estimated. Moreover, the level of malonaldehyde (MDA) was analyzed as a lipid peroxidation marker. In addition, reduced glutathione (GSH), glutathione peroxidase (GSH-Px), superoxide dismutase (SOD) and catalase (CAT) were analyzed as antioxidant biomarkers in liver, kidney and gills. Results show that deltamethrin subacute intoxication (1.46 µg/L for 28 days) increased serum AST, ALT, ALP, cholesterol, urea, uric acid, creatinine and tissue MDA. At the same time, serum total protein and albumin as well as tissue level of GSH, GSH-Px, SOD and CAT were reduced. Allicin supplemented diets enhanced all the altered serum biochemical parameters as well as tissues' lipid peroxidation and antioxidant biomarkers in a dose-dependent manner. The results suggest that feeding allicin can ameliorate deltamethrin-induced oxidative stress and might have some therapeutic properties to protect Nile tilapia on subacute deltamethrin toxicity.
authors.affiliation: Pharmacology Department, Faculty of Veterinary Medicine, Suez Canal University, Ismailia 41522, Egypt. Electronic address: abdeldaim.m@vet.suez.edu.eg.
authors.forename: Mohamed M
authors.initials: MM
authors.lastname: Abdel-Daim
authors.affiliation: Department of Internal Medicine, Infectious and Fish Diseases, Faculty of Veterinary Medicine, El Mansoura University, Egypt.
authors.forename: Nevien K M
authors.initials: NK
authors.lastname: Abdelkhalek
authors.affiliation: Department of Hygiene, Faculty of Veterinary Medicine, Suez Canal University, Ismailia 41522, Egypt.
authors.forename: Ahmed M
authors.initials: AM
authors.lastname: Hassan
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D015415:Biomarkers / Q000378:metabolism; D002374:Catalase / Q000378:metabolism; D023681:Cichlids / Q000378:metabolism; D004032:Diet; D004220:Disulfides; D005880:Gills / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D007306:Insecticides / Q000633:toxicity*; D007668:Kidney / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000378:metabolism; D008315:Malondialdehyde / Q000378:metabolism; D009570:Nitriles / Q000633:toxicity*; D018384:Oxidative Stress / Q000187:drug effects*; D011722:Pyrethrins / Q000633:toxicity*; D013441:Sulfinic Acids / Q000494:pharmacology*; D013482:Superoxide Dismutase / Q000378:metabolism; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D000975:Antioxidants; D015415:Biomarkers; D004220:Disulfides; D007306:Insecticides; D009570:Nitriles; D011722:Pyrethrins; D013441:Sulfinic Acids; D014874:Water Pollutants, Chemical; C017180:decamethrin; C006452:allicin; D008315:Malondialdehyde; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005978:Glutathione
keywords: Allicin; Antioxidant; Deltamethrin; Gills; Kidney; Liver; Oxidative stress; Tilapia