title: Tomato waste: Carotenoids content, antioxidant and cell growth activities.
abstract: The carotenoid content, antioxidant and cell growth activities of tomato waste extracts, obtained from five different tomato genotypes, was investigated. High performance liquid chromatography was used to identify and quantify the main carotenoids present in tomato waste extracts. The antioxidant activity of tomato waste extracts was tested using spectrophotometric methods, 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activity and reducing power assay. The highest DPPH scavenging activity (IC50 = 0.057 mg/ml) was obtained for Bačka extract. The Knjaz extract showed the best reducing power (IC50 = 2.12 mg/ml). Cell growth effects were determined in HeLa, MCF7 and MRC-5 cell lines by sulforhodamine B test. Anti-proliferative effects were observed in all cell lines at higher concentrations (⩾ 0.125 mg/ml). The carotenoid contents exhibited a strong correlation with antioxidant and anti-proliferation activity. The results obtained indicated that tomato waste should be regarded as potential nutraceutic resource and may be used as a functional food ingredient.
authors.affiliation: Faculty of Technology, University of Novi Sad, Bulevar Cara Lazara 1, 21000 Novi Sad, Serbia. Electronic address: sladja@uns.ac.rs.
authors.forename: Sladjana
authors.initials: S
authors.lastname: Stajčić
authors.affiliation: Faculty of Technology, University of Novi Sad, Bulevar Cara Lazara 1, 21000 Novi Sad, Serbia.
authors.forename: Gordana
authors.initials: G
authors.lastname: Ćetković
authors.affiliation: Faculty of Technology, University of Novi Sad, Bulevar Cara Lazara 1, 21000 Novi Sad, Serbia.
authors.forename: Jasna
authors.initials: J
authors.lastname: Čanadanović-Brunet
authors.affiliation: Faculty of Technology, University of Novi Sad, Bulevar Cara Lazara 1, 21000 Novi Sad, Serbia.
authors.forename: Sonja
authors.initials: S
authors.lastname: Djilas
authors.affiliation: Institute for Food Technology in Novi Sad, University of Novi Sad, Bulevar Cara Lazara 1, 21000 Novi Sad, Serbia.
authors.forename: Anamarija
authors.initials: A
authors.lastname: Mandić
authors.affiliation: Oncology Institute of Vojvodina, Faculty of Medicine, University of Novi Sad, Institutski put 4, 21204 Sremska Kamenica, Serbia.
authors.forename: Dragana
authors.initials: D
authors.lastname: Četojević-Simin
mesh_terms: D000975:Antioxidants / Q000494:pharmacology*; D002338:Carotenoids / Q000032:analysis*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D016166:Free Radical Scavengers / Q000494:pharmacology; D006801:Humans; D018551:Solanum lycopersicum / Q000737:chemistry*; D010936:Plant Extracts / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; D016166:Free Radical Scavengers; D010936:Plant Extracts; D002338:Carotenoids
keywords: Carotenoids; Cell growth activity; DPPH assay; HPLC/DAD; Reducing power; Tomato waste