title: Effects of selected xenobiotics on hepatic and plasmatic biomarkers in juveniles of Solea senegalensis.
abstract: In recent years, Solea senegalensis has increasingly been used in pollution monitoring studies. In order to assess its response to some particular widespread pollutants, juveniles of S. senegalensis were administered an intraperitoneal injection of the model aryl hydrocarbon receptor agonist β-naphtoflavone (βNF) and chemicals of environmental concern, such as the fungicide ketoconazole (KETO), the lipid regulator gemfibrozil (GEM), the surfactant nonylphenol (NP) and the synthetic hormone ethinylestradiol (EE2). Two days after injection, the effect of these chemicals was followed up as alterations of hepatic microsomal activities of the cytochrome P450 (CYPs) and associated reductases, carboxylesterases (CbEs) and the conjugation enzyme uridine diphosphate glucuronyltransferase (UDPGT). In the cytosolic fraction of the liver, the effect on CbEs, glutathione S-transferase (GST) and antioxidant activities was also considered. Alterations on the endocrine reproductive system were evaluated by plasma levels of vitellogenin (VTG) and the sex steroids estradiol (E2), testosterone (T), 11-ketotestosterone (11KT) and the progestin 17α,20β-dihydroxy-4-pregnen-3-one (17,20β-P). Injection with the model compound βNF induced the hydrolysis rate of the seven CYP substrates assayed. The xenobiotic GEM induced three CYP-related activities (e.g. ECOD) and UDPGT, but depressed antioxidant defenses. EE2 induced four CYPs, more significantly ECOD and BFCOD activities. The xenoestrogens NP and EE2 altered the activities of CbE in microsomes and catalase, and were the only treatments that induced de novo VTG synthesis. In addition, the progestin 17,20β-P, was induced in NP-injected fish. None of the treatments caused statistically significant effects on steroid plasma levels. In conclusion, the CYP substrates assayed responded specifically to treatments and juveniles of S. senegalensis appear good candidates for assessing xenobiotics exposure.
authors.affiliation: Institut de Ciencies del Mar (ICM-CSIC), Passeig Marítim de la Barceloneta 37-49, 08003 Barcelona, Spain. Electronic address: msole@icm.csic.es.
authors.forename: Montserrat
authors.initials: M
authors.lastname: Solé
authors.affiliation: Institut de Ciencies del Mar (ICM-CSIC), Passeig Marítim de la Barceloneta 37-49, 08003 Barcelona, Spain.
authors.forename: Anna
authors.initials: A
authors.lastname: Fortuny
authors.affiliation: Institute of Aquaculture Torre la Sal (IATS-CSIC), 12595 Ribera de Cabanes, Castellón, Spain.
authors.forename: Evaristo
authors.initials: E
authors.lastname: Mañanós
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000097:blood / Q000378:metabolism*; D004997:Ethinyl Estradiol / Q000008:administration & dosage / Q000494:pharmacology; D005412:Flatfishes / Q000097:blood*; D047309:Flavones / Q000008:administration & dosage / Q000494:pharmacology; D015248:Gemfibrozil / Q000008:administration & dosage / Q000494:pharmacology; D012739:Gonadal Steroid Hormones / Q000097:blood; D006868:Hydrolysis / Q000187:drug effects; D007654:Ketoconazole / Q000008:administration & dosage / Q000494:pharmacology; D008862:Microsomes, Liver / Q000187:drug effects; D010636:Phenols / Q000008:administration & dosage / Q000494:pharmacology; D018336:Receptors, Aryl Hydrocarbon / Q000819:agonists; D018709:Statistics, Nonparametric; D014819:Vitellogenins / Q000097:blood; D015262:Xenobiotics / Q000008:administration & dosage / Q000378:metabolism* / Q000494:pharmacology*
chemical_list: D015415:Biomarkers; D047309:Flavones; D012739:Gonadal Steroid Hormones; D010636:Phenols; D018336:Receptors, Aryl Hydrocarbon; D014819:Vitellogenins; D015262:Xenobiotics; D004997:Ethinyl Estradiol; C025256:nonylphenol; D015248:Gemfibrozil; D007654:Ketoconazole
keywords: Cytochrome P450; Ethinylestradiol; Gemfibrozil; Ketoconazole; Nonylphenol