title: A single step reversed-phase high performance liquid chromatography separation of polar and non-polar lipids.
abstract: This paper reports a simple chromatographic system to separate lipids classes as well as their molecular species. By the use of phenyl coated silica as stationary phase in combination with a simple mobile phase consisting of methanol and water, all tested lipid classes elute within 30 min. Furthermore, a method to accurately predict retention times of specific lipid components for this type of chromatography is presented. Common detection systems were used, namely evaporative light scattering detection (ELSD), charged aerosol detection (CAD), electrospray mass spectrometry (ESI-MS), and UV detection.
authors.affiliation: Department of Analytical Chemistry, Arrhenius Laboratory, Stockholm University, SE-106 91 Stockholm, Sweden.
authors.forename: Petter
authors.initials: P
authors.lastname: Olsson
authors.affiliation: Department of Analytical Chemistry, Arrhenius Laboratory, Stockholm University, SE-106 91 Stockholm, Sweden.
authors.forename: Jan
authors.initials: J
authors.lastname: Holmbäck
authors.affiliation: Department of Analytical Chemistry, Arrhenius Laboratory, Stockholm University, SE-106 91 Stockholm, Sweden. Electronic address: bengt.herslof@anchem.su.se.
authors.forename: Bengt
authors.initials: B
authors.lastname: Herslöf
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods; D056148:Chromatography, Reverse-Phase / Q000295:instrumentation / Q000379:methods*; D008055:Lipids / Q000302:isolation & purification*; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D008055:Lipids
keywords: Charged aerosol detector; Evaporative light scattering detector; Glycerolipids; Mass spectrometry; Phospholipids; Reversed phase liquid chromatography