title: Olfactory biosensor for insect semiochemicals analysis by impedance sensing of odorant-binding proteins on interdigitated electrodes.
abstract: Insects can sensitively and selectively detect thousands of semiochemicals at very low concentrations by their remarkable olfactory systems. As one of the most important olfactory proteins, odorant-binding proteins (OBPs) from insects are the most promising candidates for fabricating biosensors to detect biochemical molecules in the chemical ecology as well as for other biotechnological applications. In this study, we designed an olfactory biosensor by immobilizing OBPs from oriental fruit fly on interdigitated electrodes to detect semiochemicals. After successfully separated and purified, OBPs were immobilized by the special designed polyethylene glycol (PEG), SH-PEG-COOH, to produce a robust sensing membrane. Based on electrochemical sensing, interactions between OBPs and different semiochemicals emitted from host plants of the insect, such as the isoamyl acetate, β-ionone, and benzaldehyde, could be sensitively detected. With related amino acid residues in the hydrophobic cavities distinguished, the interaction forces between semiochemicals and OBPs were analyzed by molecular docking. Integrated biological olfaction proteins of insects, OBPs based biosensors could not only advance the progress in the understanding of chemical communication systems of insects, but also show promising potentials for biosensing applications in many fields.
authors.affiliation: Biosensor National Special Laboratory, Key Laboratory for Biomedical Engineering of Education Ministry, Department of Biomedical Engineering, Zhejiang University, Hangzhou 310027, P.R. China.
authors.forename: Yanli
authors.initials: Y
authors.lastname: Lu
authors.affiliation: Biosensor National Special Laboratory, Key Laboratory for Biomedical Engineering of Education Ministry, Department of Biomedical Engineering, Zhejiang University, Hangzhou 310027, P.R. China.
authors.forename: Yao
authors.initials: Y
authors.lastname: Yao
authors.affiliation: Biosensor National Special Laboratory, Key Laboratory for Biomedical Engineering of Education Ministry, Department of Biomedical Engineering, Zhejiang University, Hangzhou 310027, P.R. China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: Biosensor National Special Laboratory, Key Laboratory for Biomedical Engineering of Education Ministry, Department of Biomedical Engineering, Zhejiang University, Hangzhou 310027, P.R. China.
authors.forename: Diming
authors.initials: D
authors.lastname: Zhang
authors.affiliation: College of Environmental and Resource Sciences, Zhejiang University, Hangzhou 310058, P.R. China.
authors.forename: Shulin
authors.initials: S
authors.lastname: Zhuang
authors.affiliation: College of Life Sciences, China Jiliang University, Hangzhou 310018, P.R. China.
authors.forename: Hongliang
authors.initials: H
authors.lastname: Li
authors.affiliation: Biosensor National Special Laboratory, Key Laboratory for Biomedical Engineering of Education Ministry, Department of Biomedical Engineering, Zhejiang University, Hangzhou 310027, P.R. China. Electronic address: qjliu@zju.edu.cn.
authors.forename: Qingjun
authors.initials: Q
authors.lastname: Liu
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D015374:Biosensing Techniques; D017097:Electric Impedance; D004566:Electrodes; D055767:Immobilized Proteins / Q000737:chemistry; D019476:Insect Proteins / Q000737:chemistry*; D062105:Molecular Docking Simulation; D010675:Pheromones / Q000737:chemistry / Q000302:isolation & purification*; D018035:Receptors, Odorant / Q000737:chemistry*; D012903:Smell / Q000502:physiology
chemical_list: D055767:Immobilized Proteins; D019476:Insect Proteins; D010675:Pheromones; D018035:Receptors, Odorant; C045648:odorant-binding protein
keywords: Impedance; Insect; Odorant-binding protein; Olfactory biosensor; Semiochemical