title: Overexpression of wheat NF-YA10 gene regulates the salinity stress response in Arabidopsis thaliana.
abstract: The nuclear factor Y (NF-Y) transcription factor is formed by the interaction of three distinct subunits (NF-YA, -YB and -YC). It targets the CCAAT box, a common cis-element in eukaryotic promoters. Here, the bread wheat gene TaNF-YA10-1 has been isolated from the salinity tolerant cultivar SR3. Recombinant TaNF-YA10-1 was heterologously produced in Escherichia coli, and the purified protein successfully bound to the CCAAT motif in vitro. TaNF-YA10-1 was down-regulated by the imposition of salinity and abscisic acid (ABA). The constitutive expression of TaNF-YA10-1 in Arabidopsis thaliana significantly increased the plant's sensitivity to salinity and repressed its sensitivity to ABA as judged from the seed germination, cotyledon greening and the relative root growth. The transcription of stress-related genes AtRAB18, AtRD29B, AtABI5, AtCBF1 and AtCBF3 was downregulated in TaNF-YA10-1 overexpression transgenic plants. The data provide supportive evidence that TaNFYA10-1 is involved in the regulation of growth under salinity stress conditions.
authors.affiliation: The Key Laboratory of Plant Cell Engineering and Germplasm Innovation, Ministry of Education, School of Life Science, Shandong University, Jinan 250100, PR China.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Ma
authors.affiliation: The Key Laboratory of Plant Cell Engineering and Germplasm Innovation, Ministry of Education, School of Life Science, Shandong University, Jinan 250100, PR China.
authors.forename: Xinlei
authors.initials: X
authors.lastname: Zhu
authors.affiliation: The Key Laboratory of Plant Cell Engineering and Germplasm Innovation, Ministry of Education, School of Life Science, Shandong University, Jinan 250100, PR China.
authors.forename: Chunlong
authors.initials: C
authors.lastname: Li
authors.affiliation: The Key Laboratory of Plant Cell Engineering and Germplasm Innovation, Ministry of Education, School of Life Science, Shandong University, Jinan 250100, PR China.
authors.forename: Yinling
authors.initials: Y
authors.lastname: Song
authors.affiliation: The Key Laboratory of Plant Cell Engineering and Germplasm Innovation, Ministry of Education, School of Life Science, Shandong University, Jinan 250100, PR China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.affiliation: The Key Laboratory of Plant Cell Engineering and Germplasm Innovation, Ministry of Education, School of Life Science, Shandong University, Jinan 250100, PR China.
authors.forename: Guangmin
authors.initials: G
authors.lastname: Xia
authors.affiliation: The Key Laboratory of Plant Cell Engineering and Germplasm Innovation, Ministry of Education, School of Life Science, Shandong University, Jinan 250100, PR China. Electronic address: mwang2009@sdu.edu.cn.
authors.forename: Mei
authors.initials: M
authors.lastname: Wang
mesh_terms: D000040:Abscisic Acid / Q000494:pharmacology; D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development; D029681:Arabidopsis Proteins / Q000235:genetics; D023081:CCAAT-Binding Factor / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D004247:DNA / Q000235:genetics / Q000378:metabolism; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D018525:Germination / Q000187:drug effects / Q000235:genetics; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D018613:Microscopy, Confocal; D008969:Molecular Sequence Data; D019697:Onions / Q000166:cytology; D010802:Phylogeny; D059828:Plant Cells / Q000378:metabolism; D019441:Plant Epidermis / Q000166:cytology; D010937:Plant Growth Regulators / Q000494:pharmacology; D010940:Plant Proteins / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D018517:Plant Roots / Q000187:drug effects / Q000235:genetics / Q000254:growth & development; D030821:Plants, Genetically Modified; D011485:Protein Binding; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012639:Seeds / Q000187:drug effects / Q000235:genetics / Q000254:growth & development; D017386:Sequence Homology, Amino Acid; D012965:Sodium Chloride / Q000494:pharmacology; D014908:Triticum / Q000166:cytology / Q000235:genetics* / Q000378:metabolism
chemical_list: D029681:Arabidopsis Proteins; D023081:CCAAT-Binding Factor; D010937:Plant Growth Regulators; D010940:Plant Proteins; C402244:nuclear factor Y; D049452:Green Fluorescent Proteins; D012965:Sodium Chloride; D000040:Abscisic Acid; D004247:DNA
keywords: Abscisic acid; CCAAT-box; NF-Y transcription factor; SR3; Salinity stress; Wheat