title: Characteristics of the mosaic genome of a human parechovirus type 1 strain isolated from an infant with pneumonia in China.
abstract: Human parechoviruses (HPeVs) belong to the Parechovirus genus of the large and growing family of Picornaviridae with a non-enveloped, single-stranded and positive-sense RNA. An HPeV strain was isolated from the nasopharyngeal aspirate specimen of a 2 months old infant hospitalized with pneumonia in Beijing, China and nominated as BJ-37359 followed the code of the specimen. Strain BJ-37359 was identified as HPeV1 by whole genome sequencing. The full genome of strain BJ-37359 consisted of 7336 nucleotides (nt), excluding a poly (A) tail and contained an ORF of 6537 nt flanked by 5'UTR of 709 nt and 3'UTR of 90 nt. Phylogenetic analyses revealed that strain BJ-37359 were clustered together with HPeV1 strains in the structural capsid protein region, while uncoupling in the non-structural gene regions. Analyses with Simplot and Bootscan indicated that multiple recombination events occurred in the non-structural region and VP0 region of strain BJ-37359 with other HPeV1, and other types might have contributed to the recombination, especially HPeV6 and HPeV7 strains. Recombination analyses indicated that strain BJ-37359 may have a mosaic genome with new genomic recombination breakpoints.
authors.affiliation: Capital Institute of Pediatrics, Beijing, China.
authors.forename: Runan
authors.initials: R
authors.lastname: Zhu
authors.affiliation: Capital Institute of Pediatrics, Beijing, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Luo
authors.affiliation: Capital Institute of Pediatrics, Beijing, China.
authors.forename: Linqing
authors.initials: L
authors.lastname: Zhao
authors.affiliation: Capital Institute of Pediatrics, Beijing, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Deng
authors.affiliation: Capital Institute of Pediatrics, Beijing, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Wang
authors.affiliation: Capital Institute of Pediatrics, Beijing, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Sun
authors.affiliation: Capital Institute of Pediatrics, Beijing, China.
authors.forename: Qinwei
authors.initials: Q
authors.lastname: Song
authors.affiliation: Capital Institute of Pediatrics, Beijing, China.
authors.forename: Yaxin
authors.initials: Y
authors.lastname: Ding
authors.affiliation: Capital Institute of Pediatrics, Beijing, China. Electronic address: yqianbjc@263.net.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Qian
mesh_terms: D000818:Animals; D002681:China; D002522:Chlorocebus aethiops; D019143:Evolution, Molecular; D014644:Genetic Variation; D006801:Humans; D007223:Infant; D008297:Male; D030061:Parechovirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D010802:Phylogeny; D010850:Picornaviridae Infections / Q000821:virology*; D011024:Pneumonia, Viral / Q000821:virology*; D012367:RNA, Viral / Q000235:genetics; D011995:Recombination, Genetic; D017423:Sequence Analysis, RNA; D014709:Vero Cells
chemical_list: D012367:RNA, Viral
keywords: Human parechovirus type 1; Infantile pneumonia; Phylogenetic analysis; Recombination