title: [Ectopic osteogenesis in vivo using bone morphogenetic protein-2 derived peptide loaded biodegradable hydrogel].
abstract: We investigated the development of an injectable, biodegradable hydrogel composite of poly(trimethylene carbonate)-F127-poly(trimethylene carbonate)(PTMC11-F127-PTMC11 )loaded with bone morphogenetic protein-2 (BMP-2) derived peptide P24 for ectopic bone formation in vivo and evaluated its release kinetics in vitro. Then we evaluated P24 peptide release kinetics from different concentration of PTMC11-F127-PTMC11 hydrogel in vitro using bicinchoninic acid (BCA)assay. P24/ PTMC11-F127-PTMC11 hydrogel was implanted into each rat's erector muscle of spine and ectopic bone formation of the implanted gel in vivo was detected by hematoxylin and eosin stain (HE). PTMC11-F127-PTMC11 hydrogel with concentration more than 20 percent showed sustained slow release for one month after the initial burst release. Bone trabeculae surround the P24/ PTMC11-F127-PTMC11 hydrogel was shown at the end of six weeks by hematoxylin and eosin stain. These results indicated that encapsulated bone morphogenetic protein (BMP-2) derived peptide P24 remained viable in vivo, thus suggesting the potential of PTMC11-F127-PT- MC11 composite hydrogels as part of a novel strategy for localized delivery of bioactive molecules.
authors.forename: Jingjing
authors.initials: J
authors.lastname: Zhao
authors.forename: Zhenhua
authors.initials: Z
authors.lastname: Fang
authors.forename: Ruokun
authors.initials: R
authors.lastname: Huang
authors.forename: Kai
authors.initials: K
authors.lastname: Xiao
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.forename: Ming
authors.initials: M
authors.lastname: Xie
authors.forename: Wusheng
authors.initials: W
authors.lastname: Kan
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D019485:Bone Morphogenetic Proteins / Q000494:pharmacology*; D001842:Bone and Bones / Q000187:drug effects*; D004146:Dioxanes / Q000737:chemistry; D016503:Drug Delivery Systems; D020100:Hydrogels / Q000737:chemistry*; D010012:Osteogenesis / Q000187:drug effects*; D010455:Peptides; D019736:Prostheses and Implants; D051381:Rats
chemical_list: D001672:Biocompatible Materials; D019485:Bone Morphogenetic Proteins; D004146:Dioxanes; D020100:Hydrogels; D010455:Peptides; C443643:trimethylene carbonate