title: Antioxidant effect of erdosteine and lipoic acid in ovarian ischemia-reperfusion injury.
abstract: OBJECTIVE
  To investigate the effects of erdosteine and alpha lipoic acid (ALA) in a rat model of ovarian ischaemia-reperfusion injury.
  
  
  METHODS
  Forty-eight female Wistar albino rats were separated, at random, into six groups of eight rats. The groups were classified as: sham, torsion, detorsion, detorsion+erdosteine 100mg/kg, detorsion+alpha lipoic acid (ALA) 100mg/kg, and detorsion+erdosteine+ALA. The investigators executing the biochemical and histological analyses were blinded to the randomization until the end of the study.
  
  
  RESULTS
  The TOS (Total Oxidant Status) and OSI (Oxidative Stress Index) levels are higher in the Torsion and Detorsion groups when compared with the ones in the Sham group (p<0.05). Strong correlation was found between OSI and total histological score in the sham, torsion and detorsion groups (r=0.765, p<0.001). The mean levels of TOS and OSI in the rats that received erdosteine and/or ALA were significantly lower compared with the sham, torsion and detorsion groups (p<0.05). Mean TOS and mean OSI were lower in the detorsion+erdosteine+ALA group compared with the detorsion+erdosteine and detorsion+ALA groups (p<0.05). In comparison with the detorsion group, the numbers of primordial follicles (p=0.006) and primary follicles (p=0.036) were increased in the groups that received erdosteine and/or ALA.
  
  
  CONCLUSIONS
  Erdosteine and ALA decreased ischaemia-reperfusion injury in an experimental rat ovarian torsion model; combination treatment had a greater effect than either agent alone. Treatment with erdosteine and/or ALA was found to preserve the loss of reproductive capacity normally observed after ovarian torsion.
authors.affiliation: Department of Medical Physiology, School of Medicine, Mustafa Kemal University, Hatay, Turkey. Electronic address: drecepfatih@gmail.com.
authors.forename: R
authors.initials: R
authors.lastname: Dokuyucu
authors.affiliation: Department of Obstetrics and Gynaecology, The government hospital of obstetrics and gynecology, Hatay, Turkey.
authors.forename: A
authors.initials: A
authors.lastname: Karateke
authors.affiliation: Department of Pathology, School of Medicine, Mustafa Kemal University, Hatay, Turkey.
authors.forename: H
authors.initials: H
authors.lastname: Gokce
authors.affiliation: Department of Obstetrics and Gynaecology, School of Medicine, Mustafa Kemal University, Hatay, Turkey.
authors.forename: R K
authors.initials: RK
authors.lastname: Kurt
authors.affiliation: Department of Biochemistry, School of Medicine, Mustafa Kemal University, Hatay, Turkey.
authors.forename: O
authors.initials: O
authors.lastname: Ozcan
authors.affiliation: Department of Biochemistry, School of Medicine, Mustafa Kemal University, Hatay, Turkey.
authors.forename: S
authors.initials: S
authors.lastname: Ozturk
authors.affiliation: Department of Pathology, School of Medicine, Mustafa Kemal University, Hatay, Turkey.
authors.forename: Z A
authors.initials: ZA
authors.lastname: Tas
authors.affiliation: Department of General Surgery, Numune Training and Research Hospital, Adana, Turkey.
authors.forename: F
authors.initials: F
authors.lastname: Karateke
authors.affiliation: Department of Emergency Medicine, Faculty of Medicine, University of Mustafa Kemal, Hatay, Turkey.
authors.forename: M
authors.initials: M
authors.lastname: Duru
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology / Q000627:therapeutic use*; D004359:Drug Therapy, Combination; D005260:Female; D023421:Models, Animal; D006080:Ovarian Follicle / Q000187:drug effects / Q000473:pathology; D010053:Ovary / Q000098:blood supply* / Q000473:pathology; D018384:Oxidative Stress / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000209:etiology / Q000473:pathology / Q000517:prevention & control*; D008063:Thioctic Acid / Q000494:pharmacology / Q000627:therapeutic use*; D013864:Thioglycolates / Q000494:pharmacology / Q000627:therapeutic use*; D013876:Thiophenes / Q000494:pharmacology / Q000627:therapeutic use*; D054159:Torsion, Mechanical
chemical_list: D000975:Antioxidants; D013864:Thioglycolates; D013876:Thiophenes; D008063:Thioctic Acid; C048498:erdosteine
keywords: Alpha lipoic acid; Erdostein; Follicular heritage; Ischaemia/reperfusion; Ovarian torsion