title: Paeoniflorin alleviates liver fibrosis by inhibiting HIF-1α through mTOR-dependent pathway.
abstract: HIF-1α/mTOR signaling pathway is considered to play a crucial role in genesis and progress of tissue fibrosis. The elevation of HIF-1α and mTOR is relevant to CCl4 induced liver fibrotic rats. Paeoniflorin has been consistently shown to exhibit multiple pharmacological effects in liver disease. However, so far, no research demonstrates the relationship between paeoniflorin and HIF-1α/mTOR fibrogenesis pathway in liver fibrosis. In this study, the liver fibrosis was performed by CCl4 rats and HSC-T6 cell line. The data demonstrated that paeoniflorin treatment could attenuate liver fibrosis and inhibit the activation of HSC. Moreover, paeoniflorin significantly enhanced hepatic function by decreasing serum level of ALT, AST and ALP, and increasing level of ALB, TP. Meanwhile, ECM degradation was modulated by paeoniflorin treated rats with a remarkable reduce of α-SMA and collagen III mRNA expression. Moreover, the alleviation effect of liver fibrosis was relevant to inhibiting HIF-1α and phosphor-mTOR. Our data indicate that paeoniflorin alleviates liver fibrosis by inhibiting HIF-1α expression partly through mTOR pathway and paeoniflorin may be a potential therapeutic agent for liver fibrosis.
authors.affiliation: China Military Institute of Chinese Medicine, 302 Military Hospital of China, Beijing 100039, China. Electronic address: zhaoyl2855@126.com.
authors.forename: Yanling
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: China Military Institute of Chinese Medicine, 302 Military Hospital of China, Beijing 100039, China; Pharmacy College, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Ma
authors.affiliation: China Military Institute of Chinese Medicine, 302 Military Hospital of China, Beijing 100039, China.
authors.forename: Jiabo
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Integrative Medical Center, 302 Military Hospital of China, Beijing, 100039, China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: Department of Integrative Medical Center, 302 Military Hospital of China, Beijing, 100039, China.
authors.forename: Ruisheng
authors.initials: R
authors.lastname: Li
authors.affiliation: Pharmacy College, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu 610041, China.
authors.forename: Xuan
authors.initials: X
authors.lastname: He
authors.affiliation: Department of Medicine, 302 Military Hospital of China, Beijing 100039, China.
authors.forename: Limei
authors.initials: L
authors.lastname: Shan
authors.affiliation: Department of Integrative Medical Center, 302 Military Hospital of China, Beijing, 100039, China.
authors.forename: Ruilin
authors.initials: R
authors.lastname: Wang
authors.affiliation: Department of Integrative Medical Center, 302 Military Hospital of China, Beijing, 100039, China.
authors.forename: Lifu
authors.initials: L
authors.lastname: Wang
authors.affiliation: Department of Integrative Medical Center, 302 Military Hospital of China, Beijing, 100039, China.
authors.forename: Yonggang
authors.initials: Y
authors.lastname: Li
authors.affiliation: China Military Institute of Chinese Medicine, 302 Military Hospital of China, Beijing 100039, China. Electronic address: xiaoxiaohe302@126.com.
authors.forename: Xiaohe
authors.initials: X
authors.lastname: Xiao
mesh_terms: D000818:Animals; D002460:Cell Line; D005960:Glucosides / Q000494:pharmacology*; D055166:Hepatic Stellate Cells / Q000187:drug effects; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000378:metabolism*; D008099:Liver / Q000187:drug effects / Q000473:pathology; D008103:Liver Cirrhosis / Q000188:drug therapy*; D008297:Male; D039821:Monoterpenes / Q000494:pharmacology*; D017207:Rats, Sprague-Dawley; D015398:Signal Transduction / Q000187:drug effects; D058570:TOR Serine-Threonine Kinases / Q000378:metabolism*; D006027:Glycosides
chemical_list: D005960:Glucosides; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D039821:Monoterpenes; D058570:TOR Serine-Threonine Kinases; C497444:Hif1a protein, rat; C015423:peoniflorin; D006027:Glycosides; C546845:mTOR protein, rat
keywords: Carbon tetrachloride; Hypoxia inducible factor; Liver fibrosis; Paeoniflorin