title: Quantitative real-time PCR (qPCR)--based tool for detection and quantification of Cordyceps militaris in soil.
abstract: A quantitative real-time PCR using a primer pair CM2946F/CM3160R was developed for specific detection and quantification of Cordyceps militaris from soil. Standard curves were obtained for genomic DNA and DNA extracts from autoclaved soil with a certain dose of C. militaris suspension. C. militaris was detected from two forest soil samples out of ten that were collected when fruit bodies of C. militaris were found. This method seemed effective in detection of C. militaris in the soil and useful for rapid and reliable quantification of C. militaris in different ecosystems.
authors.affiliation: Education and Research Center, The University of Tokyo Forests, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo, Tokyo 113-8657, Japan. Electronic address: akhi_amree@yahoo.com.
authors.forename: Syaiful Amri
authors.initials: SA
authors.lastname: Saragih
authors.affiliation: The University of Tokyo Tanashi Forest, The University of Tokyo Forests, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Nishi-Tokyo, Tokyo 188-0002, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Takemoto
authors.affiliation: The University of Tokyo Chiba Forest, The University of Tokyo Forests, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Kamogawa, Chiba 299-5503, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Hisamoto
authors.affiliation: The University of Tokyo Chichibu Forest, The University of Tokyo Forests, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Chichibu, Saitama 368-0034, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Fujii
authors.affiliation: Department of Forest Entomology, Forestry and Forest Products Research Institute, Tsukuba, Ibaraki 305-8687, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Sato
authors.affiliation: The University of Tokyo Chichibu Forest, The University of Tokyo Forests, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Chichibu, Saitama 368-0034, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Kamata
mesh_terms: D032661:Cordyceps / Q000302:isolation & purification*; D004271:DNA, Fungal / Q000737:chemistry; D017753:Ecosystem; D004784:Environmental Monitoring / Q000379:methods; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods; D012988:Soil Microbiology
chemical_list: D004271:DNA, Fungal
keywords: Amplification efficiency; Culture-independent method; Entomopathogenic fungus; Genomic DNA; Soil DNA