title: Structural features and antitumor activity of a purified polysaccharide extracted from Sargassum horneri.
abstract: A polysaccharide fraction (SHPSA) was obtained from Sargassum horneri by hot-water extraction and sequential purification of anion-exchange chromatography and gel-filtration chromatography. SHPSA was found to be a neutral polysaccharide fraction with an average molecular weight of 5.78×10(5) Da and composed of T-D-Glcp, 1,3-D-Glcp, 1,6-D-Glcp and 1,3,6-D-Glcp in a molar percentage of 1.00:4.17:1.17:0.89, respectively. Based on the results from chemical analysis, NMR, and SHPSA was determined to be a glucan with β-(1→6) side chains linked to a β-(1→3) backbone with relatively few branch points. Moreover, SHPSA could inhibit the growth of human colon cancer DLD cells in a dose-dependent manner by inducing the apoptosis of DLD cells. So, SHPSA was promising for future use as a natural antitumor agent.
authors.affiliation: Department of Food Science and Technology, Zhejiang University of Technology, Hangzhou, Zhejiang 310014, PR China. Electronic address: pingshao325@163.com.
authors.forename: Ping
authors.initials: P
authors.lastname: Shao
authors.affiliation: Department of Food Science and Technology, Zhejiang University of Technology, Hangzhou, Zhejiang 310014, PR China.
authors.forename: Jia
authors.initials: J
authors.lastname: Liu
authors.affiliation: Department of Food Science and Technology, Zhejiang University of Technology, Hangzhou, Zhejiang 310014, PR China.
authors.forename: Xiaoxiao
authors.initials: X
authors.lastname: Chen
authors.affiliation: School of Public Health, Curtin Health Innovation Research Institute, Curtin University, GPO Box U1987, Perth, 6845 WA, Australia.
authors.forename: Zhongxiang
authors.initials: Z
authors.lastname: Fang
authors.affiliation: Department of Food Science and Technology, Zhejiang University of Technology, Hangzhou, Zhejiang 310014, PR China.
authors.forename: Peilong
authors.initials: P
authors.lastname: Sun
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D045744:Cell Line, Tumor; D006801:Humans; D008745:Methylation; D008970:Molecular Weight; D019906:Nuclear Magnetic Resonance, Biomolecular; D010936:Plant Extracts / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D044647:Sargassum / Q000737:chemistry*
chemical_list: D000970:Antineoplastic Agents; D010936:Plant Extracts; D011134:Polysaccharides
keywords: Anti-tumor activity; Sargassum horneri polysaccharide; Structural characterization