title: Effective stimulating factors for microbial levan production by Halomonas smyrnensis AAD6T.
abstract: Levan is a bioactive fructan polymer that is mainly associated with high-value applications where exceptionally high purity requirements call for well-defined cultivation conditions. In this study, microbial levan production by the halophilic extremophile Halomonas smyrnensis AAD6(T) was investigated systematically. For this, different feeding strategies in fed-batch cultures were employed and fermentation profiles of both shaking and bioreactor cultures were analyzed. Initial carbon and nitrogen source concentrations, production pH, NaCl and nitrogen pulses, nitrogen and phosphorous limitations, trace elements and thiamine contents of the basal production medium were found to affect the levan yields at different extends. Boric acid was found to be the most effective stimulator of levan production by increasing the sucrose utilization three-fold and levan production up to five-fold. This significant improvement implied the important role of quorum sensing phenomenon and its regulatory impact on levan production mechanism. Levan produced by bioreactor cultures under conditions optimized within this study was found to retain its chemical structure. Moreover, its biocompatibility was assessed for a broad concentration range. Hence H. smyrnensis AAD6(T) has been firmly established as an industrially important resource microorganism for high-quality levan production.
authors.affiliation: Department of Bioengineering, Marmara University, Goztepe Campus, 34722 Istanbul, Turkey.
authors.forename: Hande Kazak
authors.initials: HK
authors.lastname: Sarilmiser
authors.affiliation: Department of Bioengineering, Marmara University, Goztepe Campus, 34722 Istanbul, Turkey.
authors.forename: Ozlem
authors.initials: O
authors.lastname: Ates
authors.affiliation: Department of Bioengineering, Marmara University, Goztepe Campus, 34722 Istanbul, Turkey.
authors.forename: Gonca
authors.initials: G
authors.lastname: Ozdemir
authors.affiliation: Department of Bioengineering, Marmara University, Goztepe Campus, 34722 Istanbul, Turkey.
authors.forename: Kazim Yalcin
authors.initials: KY
authors.lastname: Arga
authors.affiliation: Department of Bioengineering, Marmara University, Goztepe Campus, 34722 Istanbul, Turkey. Electronic address: ebru.toksoy@marmara.edu.tr.
authors.forename: Ebru Toksoy
authors.initials: ET
authors.lastname: Oner
mesh_terms: D061249:Batch Cell Culture Techniques / Q000379:methods*; D019149:Bioreactors; D001888:Boric Acids / Q000494:pharmacology; D002244:Carbon / Q000378:metabolism; D005285:Fermentation / Q000187:drug effects; D005630:Fructans / Q000096:biosynthesis*; D020632:Halomonas / Q000187:drug effects* / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D009584:Nitrogen / Q000378:metabolism; D010758:Phosphorus / Q000378:metabolism; D053038:Quorum Sensing; D012965:Sodium Chloride / Q000378:metabolism / Q000494:pharmacology; D013395:Sucrose / Q000378:metabolism; D013831:Thiamine / Q000378:metabolism / Q000494:pharmacology; D014131:Trace Elements / Q000378:metabolism / Q000494:pharmacology
chemical_list: D001888:Boric Acids; D005630:Fructans; D014131:Trace Elements; D010758:Phosphorus; D012965:Sodium Chloride; D013395:Sucrose; D002244:Carbon; C072599:levan; D009584:Nitrogen; C032688:boric acid; D013831:Thiamine
keywords: Boric acid; Exopolysaccharides; Fructan; Halomonas smyrnensis; Levan; Quorum sensing