title: Probing small-molecule microarrays with tagged proteins in cell lysates.
abstract: The technique of small-molecule microarray (SMM) screening is based on the ability of small molecules to bind to various soluble proteins. This type of interaction is easily detected by the presence of a fluorescence signal produced by labeled antibodies that specifically recognize a unique sequence (tag) present on the target protein. The fluorescent signal intensity values are determined based on signal-to-noise ratios (SNRs). SMM screening is a high-throughput, unbiased method that can rapidly identify novel direct ligands for various protein targets. This binding-based assay format is generally applicable to most proteins, but it is especially useful for protein targets that do not possess an enzymatic activity. SMMs enable screening a protein in a purified form or in the context of a cellular lysate, likely providing a more physiologically relevant screening environment.
authors.affiliation: Broad Institute of Harvard and MIT, Cambridge, Massachusetts.|The Dana-Farber Cancer Institute, Boston, Massachusetts.
authors.forename: Marius S
authors.initials: MS
authors.lastname: Pop
authors.affiliation: Broad Institute of Harvard and MIT, Cambridge, Massachusetts.
authors.forename: Dina
authors.initials: D
authors.lastname: Wassaf
authors.affiliation: Broad Institute of Harvard and MIT, Cambridge, Massachusetts.|Koch Institute for Integrative Cancer Research at MIT, Cambridge, Massachusetts.
authors.forename: Angela N
authors.initials: AN
authors.lastname: Koehler
mesh_terms: D002457:Cell Extracts / Q000737:chemistry*; D005455:Fluorescent Antibody Technique / Q000379:methods; D006801:Humans; D008024:Ligands; D040081:Protein Array Analysis / Q000379:methods; D011485:Protein Binding; D011506:Proteins / Q000737:chemistry* / Q000302:isolation & purification; D054852:Small Molecule Libraries / Q000737:chemistry*
chemical_list: D002457:Cell Extracts; D008024:Ligands; D011506:Proteins; D054852:Small Molecule Libraries
keywords: binding assay; cellular lysate; fluorescence; protein complex; small-molecule microarray