title: Hypoxia-inducible factor-1alpha regulates autophagy to activate hepatic stellate cells.
abstract: The role of autophagy in Hif-1α modulated activation of hepatic stellate cells was illustrated in current work. Autophagy markers were determined in livers of Schistosoma japonicum infected mice and hypoxia or LPS treated human hepatic stellate cell, LX-2 cells. The action of Hif-1 to autophagy was defined as increase of autophagy markers was significantly suppressed in Hif-1α siRNA transfected cells upon hypoxia or LPS stimulation. The function of autophagy in activation of LX-2 cells was assessed as increase of activation markers was blocked using autophagy inhibitors under hypoxia and LPS stimulation. Conclusively, Hif-1α regulates activation of hepatic stellate cell by modulating autophagy.
authors.affiliation: Department of Pathogen Biology, School of Basic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, PR China.
authors.forename: Jing
authors.initials: J
authors.lastname: Deng
authors.affiliation: Department of Medical Rehabilitation, Union Hospital, Huazhong University of Science and Technology, Wuhan 430030, PR China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Huang
authors.affiliation: Department of Pathogen Biology, School of Basic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, PR China.
authors.forename: Yueqin
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Pathogen Biology, School of Basic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, PR China.
authors.forename: Pei
authors.initials: P
authors.lastname: Shen
authors.affiliation: Department of Parasitology, School of Basic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, PR China.
authors.forename: Fei
authors.initials: F
authors.lastname: Guan
authors.affiliation: Department of Pathogen Biology, School of Basic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, PR China.
authors.forename: Jianrong
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Pathogen Biology, School of Basic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, PR China.
authors.forename: Hanju
authors.initials: H
authors.lastname: Huang
authors.affiliation: Department of Pathogen Biology, School of Basic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, PR China. Electronic address: chunweishi@mail.hust.edu.cn.
authors.forename: Chunwei
authors.initials: C
authors.lastname: Shi
mesh_terms: D000818:Animals; D001343:Autophagy; D015687:Cell Hypoxia; D002460:Cell Line; D005260:Female; D055166:Hepatic Stellate Cells / Q000276:immunology* / Q000382:microbiology / Q000473:pathology; D006801:Humans; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000276:immunology*; D008070:Lipopolysaccharides / Q000276:immunology; D008099:Liver / Q000276:immunology / Q000382:microbiology / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C; D012549:Schistosoma japonicum / Q000276:immunology; D012554:Schistosomiasis japonica / Q000276:immunology / Q000473:pathology
chemical_list: D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D008070:Lipopolysaccharides
keywords: Autophagy; Hepatic stellate cells; Hif-1α; Liver fibrosis