title: Contrasted responses of Ruditapes decussatus (filter and deposit feeding) and Loripes lacteus (symbiotic) exposed to polymetallic contamination (Port-Camargue, France).
abstract: The use of symbiotic bivalve species to assess the effect of anthropogenic metal pollution was rarely investigated whereas data on filter feeding bivalves are common. The aim of this study was the exposure of two bivalve species, Ruditapes decussatus and Loripes lacteus to polymetallic pollution gradient, originating from harbor activities (Port-Camargue, south of France). Both bivalves differ by their trophic status, filter and deposit feeder for Ruditapes and symbiotic for Loripes that underlies potential differences in metal sensibility. The bivalves were immerged in July (for Ruditapes during 2 and 8 days) and in August 2012 (for Loripes during 2, 6 and 8 days) in the water column of the harbor, at 3 stations according to pollution gradient. Metal concentrations (Cu, Mn, Zn) in the water column were quantified as dissolved metals (measured by ICP-MS) and as labile metals (measured by ICP-MS using DGT technique). For each exposure time, accumulation of metals in the soft tissue of bivalves ("bioaccumulation") was measured for both species. In addition, specific parameters, according to the trophic status of each bivalve, were investigated: filtering activity (specific clearance rate, SCR) for Ruditapes, and relative cell size (SSC) and genomic content (FL1) of bacterial symbionts hosted in the gills of Loripes. The SCR of Ruditapes drops from 100% (control) to 34.7% after 2 days of exposure in the less contaminated site (station 8). On the other hand, the relative cell size (SSC) and genomic content (FL1), measured by flow cytometry were not impacted by the pollution gradient. Bioaccumulation was compared for both species, showing a greater capability of Cu accumulation for Loripes without lethal effect. Mn, Fe and Zn were generally not accumulated by any of the species according to the pollution gradient. The trophic status of each species may greatly influence their respective responses to polymetallic pollution.
authors.affiliation: Laboratoire Ecosystèmes Marins Côtiers, UMR-CNRS 5119, Université Montpellier II, 34095 Montpellier Cedex 5, France. Electronic address: audrey.caro@univ-montp2.fr.
authors.forename: Audrey
authors.initials: A
authors.lastname: Caro
authors.affiliation: Laboratoire Ecosystèmes Marins Côtiers, UMR-CNRS 5119, Université Montpellier II, 34095 Montpellier Cedex 5, France.
authors.forename: Gaetan
authors.initials: G
authors.lastname: Chereau
authors.affiliation: Laboratoire HydroSciences, UMR 5569, CNRS, Universités Montpellier I and II, IRD, Place Eugène Bataillon, CC MSE, 34095 Montpellier Cedex 5, France.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Briant
authors.affiliation: Laboratoire Ecosystèmes Marins Côtiers, UMR-CNRS 5119, Université Montpellier II, 34095 Montpellier Cedex 5, France.
authors.forename: Cécile
authors.initials: C
authors.lastname: Roques
authors.affiliation: Laboratoire HydroSciences, UMR 5569, CNRS, Universités Montpellier I and II, IRD, Place Eugène Bataillon, CC MSE, 34095 Montpellier Cedex 5, France.
authors.forename: Rémi
authors.initials: R
authors.lastname: Freydier
authors.affiliation: Laboratoire HydroSciences, UMR 5569, CNRS, Universités Montpellier I and II, IRD, Place Eugène Bataillon, CC MSE, 34095 Montpellier Cedex 5, France.
authors.forename: Sophie
authors.initials: S
authors.lastname: Delpoux
authors.affiliation: Laboratoire Ecosystèmes Marins Côtiers, UMR-CNRS 5119, Université Montpellier II, 34095 Montpellier Cedex 5, France.
authors.forename: Arthur
authors.initials: A
authors.lastname: Escalas
authors.affiliation: Laboratoire HydroSciences, UMR 5569, CNRS, Universités Montpellier I and II, IRD, Place Eugène Bataillon, CC MSE, 34095 Montpellier Cedex 5, France.
authors.forename: Françoise
authors.initials: F
authors.lastname: Elbaz-Poulichet
mesh_terms: D000818:Animals; D049872:Bivalvia / Q000502:physiology*; D004784:Environmental Monitoring; D005602:France; D008668:Metallothionein / Q000378:metabolism; D008670:Metals / Q000032:analysis / Q000378:metabolism* / Q000633:toxicity; D014874:Water Pollutants, Chemical / Q000032:analysis / Q000378:metabolism* / Q000633:toxicity
chemical_list: D008670:Metals; D014874:Water Pollutants, Chemical; D008668:Metallothionein
keywords: Bivalves; Clearance rate; DGT (diffusion gradient in thin film technique); Genomic content; Metallic pollution; Relative symbiont size