title: Label electrochemical immunosensor for prostate-specific antigen based on graphene and silver hybridized mesoporous silica.
abstract: Prostate-specific antigen (PSA), as the specificity of prostate cancer markers, has been widely used in prostate cancer diagnosis and screening. In this study, we fabricated an electrochemical immunosensor for PSA detection using the amino-functionalized graphene sheet-ferrocenecarboxaldehyde composite materials (NH2-GS@FCA) and silver hybridized mesoporous silica nanoparticles (Ag@NH2-MCM48). Under optimal conditions, the fabricated immunosensor showed a wide linear range with PSA concentration (0.01-10.0ng·ml(-1)). Low detection limit (2pg·ml(-1)) proved the high sensitivity. In addition, the immunosensor possessed good stability and reproducibility. Moreover, the application to PSA analysis in serum samples yielded satisfactory results.
authors.affiliation: School of Chemical Engineering, Shandong University of Technology, Zibo 255049, People's Republic of China. Electronic address: liyueyun71@163.com.
authors.forename: Yueyun
authors.initials: Y
authors.lastname: Li
authors.affiliation: School of Chemical Engineering, Shandong University of Technology, Zibo 255049, People's Republic of China.
authors.forename: Jian
authors.initials: J
authors.lastname: Han
authors.affiliation: School of Chemical Engineering, Shandong University of Technology, Zibo 255049, People's Republic of China.
authors.forename: Runhai
authors.initials: R
authors.lastname: Chen
authors.affiliation: Key Laboratory of Chemical Sensing and Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, People's Republic of China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Ren
authors.affiliation: Key Laboratory of Chemical Sensing and Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, People's Republic of China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Wei
mesh_terms: D004566:Electrodes; D004797:Enzyme-Linked Immunosorbent Assay; D006108:Graphite / Q000737:chemistry*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008297:Male; D053758:Nanoparticles / Q000737:chemistry*; D016062:Porosity; D017430:Prostate-Specific Antigen / Q000097:blood*; D012822:Silicon Dioxide / Q000737:chemistry*
chemical_list: D012822:Silicon Dioxide; D006108:Graphite; D017430:Prostate-Specific Antigen
keywords: Immunosensor; Prostate-specific antigen; Silver hybridized mesoporous silica nanoparticles