title: A novel oxido-viscosifying Hyaluronic Acid-antioxidant conjugate for osteoarthritis therapy: biocompatibility assessments.
abstract: To overcome the problem of fast degradation of Hyaluronic Acid (HA) in the treatment of osteoarthritis (OA), HA was protected against the oxidative stress generated by the pathology. Antioxidant conjugated HAs were synthesized and tested in vitro for their resistance in an oxidative environment mimicking OA. HA-4-aminoresorcinol (HA-4AR) displayed the interesting property of increasing in viscosity under oxidative conditions because of crosslinking induced by electron transfer. The novel HA polymer conjugate was shown to be biocompatible in vitro on fibroblast-like synoviocytes extracted from an arthritic patient. This HA conjugate was also assessed in vivo by intra-articular injection in healthy rabbits and was found to be comparable to the native polymer in terms of biocompatibility. This study suggests that HA-4AR is a promising candidate for a next generation viscosupplementation formulation.
authors.affiliation: School of Pharmaceutical Sciences, University of Geneva and Lausanne, Geneva, Switzerland. Electronic address: sema.kaderli@unige.ch.
authors.forename: S
authors.initials: S
authors.lastname: Kaderli
authors.affiliation: UPSP ICE 2011.03.101, Université de Lyon, VetAgro Sup, Lyon, France. Electronic address: caroline.boulocher@vetagro-sup.fr.
authors.forename: C
authors.initials: C
authors.lastname: Boulocher
authors.affiliation: UPSP ICE 2011.03.101, Université de Lyon, VetAgro Sup, Lyon, France. Electronic address: elodie.pillet@vetagro-sup.fr.
authors.forename: E
authors.initials: E
authors.lastname: Pillet
authors.affiliation: UPSP ICE 2011.03.101, Université de Lyon, VetAgro Sup, Lyon, France. Electronic address: dorothee.watrelot@vetagro-sup.fr.
authors.forename: D
authors.initials: D
authors.lastname: Watrelot-Virieux
authors.affiliation: UPSP ICE 2011.03.101, Université de Lyon, VetAgro Sup, Lyon, France. Electronic address: thierry.roger@vetagro-sup.fr.
authors.forename: T
authors.initials: T
authors.lastname: Roger
authors.affiliation: UPSP ICE 2011.03.101, Université de Lyon, VetAgro Sup, Lyon, France. Electronic address: eric.viguier@vetagro-sup.fr.
authors.forename: E
authors.initials: E
authors.lastname: Viguier
authors.affiliation: School of Pharmaceutical Sciences, University of Geneva and Lausanne, Geneva, Switzerland. Electronic address: robert.gurny@unige.ch.
authors.forename: R
authors.initials: R
authors.lastname: Gurny
authors.affiliation: School of Pharmaceutical Sciences, University of Geneva and Lausanne, Geneva, Switzerland. Electronic address: leonardo.scapozza@unige.ch.
authors.forename: L
authors.initials: L
authors.lastname: Scapozza
authors.affiliation: School of Pharmaceutical Sciences, University of Geneva and Lausanne, Geneva, Switzerland. Electronic address: michael.moeller@unige.ch.
authors.forename: M
authors.initials: M
authors.lastname: Möller
mesh_terms: D000368:Aged; D000818:Animals; D000975:Antioxidants / Q000737:chemistry* / Q000494:pharmacology*; D001672:Biocompatible Materials / Q000737:chemistry* / Q000494:pharmacology*; D002626:Chemistry, Pharmaceutical / Q000379:methods; D005347:Fibroblasts / Q000187:drug effects; D006801:Humans; D006820:Hyaluronic Acid / Q000737:chemistry* / Q000494:pharmacology*; D007270:Injections, Intra-Articular / Q000379:methods; D007719:Knee Joint / Q000187:drug effects; D010003:Osteoarthritis / Q000188:drug therapy*; D018384:Oxidative Stress / Q000187:drug effects; D011817:Rabbits; D014783:Viscosity
chemical_list: D000975:Antioxidants; D001672:Biocompatible Materials; D006820:Hyaluronic Acid
keywords: 2-Aminophenol (PubChem CID: 5801); 4-Aminoresorcinol (PubChem CID: 432827); 4-Aminosalicylic acid (PubChem CID: 4649); 5-Aminosalicylic acid (PubChem CID: 4075); Anilin (PubChem CID: 6115); Antioxidants; Biocompatibility; Crosslinking; Hyaluronic Acid; Hyaluronic Acid (PubChem CID: 24728612); Osteoarthritis; Tyramine (PubChem CID: 66449); Viscosupplementation; l-Ethylester cystein (PubChem CID: 13359)