title: Immunolocalization of RhoA and RhoB GTPases in pleomorphic adenoma of the parotid.
abstract: The pleomorphic adenoma of the parotid (PA) is characterized by the high tissues diversity. Rho GTPases participate in signal transduction pathways that regulate several biological processes, including cell differentiation. A quantitative analysis of RhoA and RhoB GTPases immunoexpression was performed in healthy parotids and in 23 PA cases, predominantly epithelial (PE) or mesenchymal (PM), followed by Student's t test. In PE cases, RhoA immunoexpression was higher in sheets and trabeculae (p < 0.05), whereas RhoB only in sheets (p < 0.05). In normal parotids, RhoA and RhoB were not detected in acinar cells. Ducts have expressed RhoA and RhoB in normal parotids and PA. RhoB was detected in myxoid and chondromyxoid cells. Normal parotids do not express RhoA and RhoB proteins in acinar cells, indicating a lack of function in secretory cells. Despite RhoA and RhoB GTPases are different in their biological roles, no significant difference in immunoexpression of the RhoA and RhoB GTPases in epithelial and mesenchymal structures of PA.
authors.affiliation: Structural Biology Department, Institute of Natural and Biological Sciences, Federal University of Triângulo Mineiro, Uberaba, MG, Brazil.
authors.forename: Simone de Sales Costa Moreira
authors.initials: Sde S
authors.lastname: Carboni
authors.forename: Adilha Misson Rua
authors.initials: AM
authors.lastname: Micheletti
authors.forename: Nanci Mendes
authors.initials: NM
authors.lastname: Pinheiro
authors.forename: Nathália Alves Rodrigues
authors.initials: NA
authors.lastname: Lima
authors.forename: Camilla Christian Gomes
authors.initials: CC
authors.lastname: Moura
authors.forename: Fabrizio Antonio Gomide
authors.initials: FA
authors.lastname: Cardoso
authors.forename: Virgínia Oliveira
authors.initials: VO
authors.lastname: Crema
mesh_terms: D008949:Adenoma, Pleomorphic / Q000235:genetics* / Q000473:pathology; D002454:Cell Differentiation / Q000235:genetics; D004847:Epithelial Cells / Q000378:metabolism; D006801:Humans; D010306:Parotid Gland / Q000473:pathology; D015398:Signal Transduction; D020741:rho GTP-Binding Proteins / Q000096:biosynthesis*; D020742:rhoA GTP-Binding Protein / Q000096:biosynthesis*; D020743:rhoB GTP-Binding Protein / Q000096:biosynthesis*
chemical_list: C066737:RHOA protein, human; D020741:rho GTP-Binding Proteins; D020742:rhoA GTP-Binding Protein; D020743:rhoB GTP-Binding Protein