title: Designing and troubleshooting immunopanning protocols for purifying neural cells.
abstract: Purifying and culturing cells from the central nervous system (CNS) has proved to be an incredibly powerful tool for dissecting fundamental neuron and glial properties, and especially powerful in understanding neuronal-glial interactions. In a series of detailed protocols, we have provided step-by-step instructions for purifying and culturing specific types of neurons, glia, and vascular cells from the CNS by immunopanning. This article discusses common pitfalls and errors as well as important design considerations for the immunopanning procedure.
authors.affiliation: Stanford University School of Medicine, Stanford, California 94305.
authors.forename: Ben A
authors.initials: BA
authors.lastname: Barres
mesh_terms: D000818:Animals; D000906:Antibodies / Q000378:metabolism; D002469:Cell Separation / Q000379:methods*; D003470:Culture Media; D007158:Immunologic Techniques / Q000379:methods*; D009474:Neurons / Q000166:cytology*
chemical_list: D000906:Antibodies; D003470:Culture Media