title: The novel gene pFAM134B positively regulates fat deposition in the subcutaneous fat of Sus scrofa.
abstract: In this study, we analyzed the global gene expression profiles in the subcutaneous fat (SAT) of Jinhua pigs and Landrace pigs at 90d. Several genes were significantly highly expressed in Jinhua pigs, including genes encoding the rate limiting enzymes in the TCA cycle, fatty acid activation, fatty acid synthesis and triglyceride synthesis. We identified a novel gene tagged by the EST sequences as public No. BF702245.1, which was named porcine FAM134B (pFAM134B) and the pFAM134B mRNA levels of SAT was significantly higher in Jinhua pigs than that in Landrace pigs at 90d (P<0.01). Then the effects of pFAM134B on lipid accumulation were investigated by using RNAi and gene overexpression in the subcutaneous adipocytes. The results showed that pFAM134B played a significant positive role in regulating lipid deposition by increasing the mRNA levels of PPARγ, lipogenic genes fatty acid synthetase (FAS) and acetyl-CoA carboxylase (ACC) (P<0.01) and reducing the mRNA levels of adipose triglyceride lipase (ATGL) and lipase, hormone-sensitive (HSL) (P<0.01). This study implied that pFAM134B might be a positive factor in lipid deposition, providing insight into the control of fat accumulation and lipid-related disorders.
authors.affiliation: Institute of Feed Science, Zhejiang University, 866 Yuhangtang Road, Hangzhou, Zhejiang 310058, PR China.
authors.forename: ZhangQin
authors.initials: Z
authors.lastname: Yuan
authors.affiliation: Institute of Feed Science, Zhejiang University, 866 Yuhangtang Road, Hangzhou, Zhejiang 310058, PR China.
authors.forename: DeGuang
authors.initials: D
authors.lastname: Song
authors.affiliation: Institute of Feed Science, Zhejiang University, 866 Yuhangtang Road, Hangzhou, Zhejiang 310058, PR China. Electronic address: yzwang321@zju.edu.cn.
authors.forename: YiZhen
authors.initials: Y
authors.lastname: Wang
mesh_terms: D000818:Animals; D003001:Cloning, Molecular; D009363:Neoplasm Proteins / Q000235:genetics / Q000378:metabolism*; D050151:Subcutaneous Fat / Q000378:metabolism*; D034421:Sus scrofa / Q000235:genetics* / Q000378:metabolism
chemical_list: D009363:Neoplasm Proteins
keywords: Fat deposition; Gene function; Microarray; Pigs; pFAM134B