title: The ABC transporter, AbcB3, mediates cAMP export in D. discoideum development.
abstract: Extracellular cAMP functions as a primary ligand for cell surface cAMP receptors throughout Dictyostelium discoideum development, controlling chemotaxis and morphogenesis. The developmental consequences of cAMP signaling and the metabolism of cAMP have been studied in great detail, but it has been unclear how cells export cAMP across the plasma membrane. Here we show pharmacologically and genetically that ABC transporters mediate cAMP export. Using an evolutionary-developmental biology approach, we identified several candidate abc genes and characterized one of them, abcB3, in more detail. Genetic and biochemical evidence suggest that AbcB3 is a component of the cAMP export mechanism in D. discoideum development.
authors.affiliation: Graduate Program in Developmental Biology, Baylor College of Medicine, Houston, TX 77030, USA; Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX 77030, USA.
authors.forename: Edward Roshan
authors.initials: ER
authors.lastname: Miranda
authors.affiliation: Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX 77030, USA.
authors.forename: Edward A
authors.initials: EA
authors.lastname: Nam
authors.affiliation: Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX 77030, USA; Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, TX 77030, USA.
authors.forename: Adam
authors.initials: A
authors.lastname: Kuspa
authors.affiliation: Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX 77030, USA. Electronic address: gadi@bcm.edu.
authors.forename: Gad
authors.initials: G
authors.lastname: Shaulsky
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000235:genetics / Q000378:metabolism*; D002633:Chemotaxis / Q000502:physiology*; D000242:Cyclic AMP / Q000378:metabolism*; D004023:Dictyostelium / Q000254:growth & development*; D055785:Gene Knockdown Techniques; D009024:Morphogenesis / Q000502:physiology*; D034622:RNA Interference; D015398:Signal Transduction / Q000502:physiology
chemical_list: D018528:ATP-Binding Cassette Transporters; D000242:Cyclic AMP
keywords: ATP-binding cassette transporters; Dictyostelium; cAMP