title: [Analysis of frequency of a RHD1227A allele in Chinese Hans].
abstract: OBJECTIVE
  To determine the frequency of RHD1227A allele in Chinese Hans.
  
  
  METHODS
  For a total of 890403 ethnic Han blood donors, the D antigen was determined with a saline method and indirect antiglobulin test. The RHD1227A allele and number and type of zygosity of RHD gene were determined with PCR sequence specific primer (PCR-SSP). Allelic frequency was calculated through statistics.
  
  
  RESULTS
  In total 2385 donors were found to be Rh-negative, 108 individuals were found to be weakly positive for D antigen (including weak D and partial D phenotypes). The remaining 887 910 individuals were Rh-positive. Among the Rh-negative individuals, 516 were found with RHD1227A. Among these, 467 were RHD1227A/d and 49 were RHD1227A/RHD1227A. Two of 108 D antigen weak-positive individuals were found as RHD1227A/RHD+. In addition, 8 of 1073 random Rh-positive samples were found to be RHD1227A/RHD+. The allele frequency of RHD1227A in the population was calculated as 0.004 036. The figure should be 0.006 682 if calculated based on the detected rate of the allele in Rh-negative individuals, and 0.007 884 if calculated based on the reported average phenotype rate of DEL in Rh-negative individuals.
  
  
  CONCLUSIONS
  By taking main influencing factors such as the RHD zygosity, the rate of RHD1227A and DEL phenotype may be determined. The allele frequency of RHD1227A in Chinese Hans is between 0.004 036 and 0.007 884.
authors.affiliation: Shenzhen Blood Center, Shenzhen, Guangdong 518035, P. R. China.drxuhua@163.com; cpshaos@hotmail.com.
authors.forename: Xiaoying
authors.initials: X
authors.lastname: Wu
authors.forename: Dazhou
authors.initials: D
authors.lastname: Wu
authors.forename: Manni
authors.initials: M
authors.lastname: Wang
authors.forename: Chaopeng
authors.initials: C
authors.lastname: Shao
authors.forename: Hua
authors.initials: H
authors.lastname: Xu
mesh_terms: D000293:Adolescent; D000328:Adult; D044466:Asian People / Q000208:ethnology / Q000235:genetics*; D002681:China / Q000208:ethnology; D005091:Exons; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D020125:Mutation, Missense; D012204:Rh-Hr Blood-Group System / Q000235:genetics*; D055815:Young Adult
chemical_list: D012204:Rh-Hr Blood-Group System; C026410:Rho(D) antigen