title: Long noncoding RNA PCA3 gene promoter region is related to the risk of prostate cancer on Chinese males.
abstract: BACKGROUND
  Long noncoding RNA prostate cancer gene antigen 3 (PCA3) is one of the most prostate cancer-specific genes at present. Consequently, the prostate-specific expression and the sharp up-regulation of PCA3 RNA in prostate cancer suggest a unique transcriptional regulation, which possibly can be attributed to promoter polymorphism. In this study, we investigated a short tandem repeat (STR) polymorphism of TAAA in the promoter region of PCA3 gene found in our previous study in prostate cancer (PCa) patients and benign prostatic hypertrophy (BPH) patients, aiming to evaluate the association between the STR and increased risk for PCa.
  
  
  METHODS
  120 PCa cases and 120 benign prostatic hypertrophy (BPH) cases were identified among participants. The region encompassing the TAAA repeat was amplified with a specific primer set we designed and screened by PCR-based cloning and sequencing in paired peripheral blood leukocytes and prostate tissues. Genotype-specific risks were estimated as odds ratios (ORs) associated with 95% confidence intervals (CIs) and adjusted for age by means of unconditional logistic regression.
  
  
  RESULTS
  5 PCA3 TAAA STR polymorphisms and 8 genotypes were found in both peripheral blood leukocytes and prostate tissues, the carriers with more TAAA repeats were associated with increased risk for PCa than individuals having less TAAA repeats. Interestingly, 18 (15.0%) of 120 PCa patients had more (TAAA)n repeats in prostate tissues than that in peripheral blood leukocytes, and 3 (2.5%) of 120 had less (TAAA)n repeats in prostate tissues.
  
  
  CONCLUSIONS
  The results of this study suggest that short tandem repeat polymorphism of TAAA in the promoter region of PCA3 gene is a risk-increasing factor for prostate cancer in the Chinese population. In addition to the hereditary factor, the insertion mutation of (TAAA)n in a local tissue maybe another mechanism of the onset of PCa.
authors.affiliation: Department of Medical Laboratory, The First Affiliated Hospital of Wenzhou Medical University, Zhejiang, PR China. Electronic address: gonewithwind95@163.com.
authors.forename: Wu
authors.initials: W
authors.lastname: Zhou
authors.affiliation: Department of Medical Laboratory, The Second Affiliated Hospital of Zhejiang University School of Medicine, Zhejiang, PR China.
authors.forename: Zhihua
authors.initials: Z
authors.lastname: Tao
authors.affiliation: Department of Medical Laboratory, The First Affiliated Hospital of Wenzhou Medical University, Zhejiang, PR China.
authors.forename: Zhongyong
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Department of Medical Laboratory, The First Affiliated Hospital of Wenzhou Medical University, Zhejiang, PR China.
authors.forename: Wangqiang
authors.initials: W
authors.lastname: Hu
authors.affiliation: Department of Medical Laboratory, The First Affiliated Hospital of Wenzhou Medical University, Zhejiang, PR China.
authors.forename: Mo
authors.initials: M
authors.lastname: Shen
authors.affiliation: Department of Medical Laboratory, The First Affiliated Hospital of Wenzhou Medical University, Zhejiang, PR China.
authors.forename: Lianlian
authors.initials: L
authors.lastname: Zhou
authors.affiliation: Department of Urologic Surgery, The First Affiliated Hospital of Wenzhou Medical University, Zhejiang, PR China.
authors.forename: Zhiliang
authors.initials: Z
authors.lastname: Wen
authors.affiliation: Department of Urologic Surgery, The First Affiliated Hospital of Wenzhou Medical University, Zhejiang, PR China.
authors.forename: Zhixian
authors.initials: Z
authors.lastname: Yu
authors.affiliation: Department of Pathology, The First Affiliated Hospital of Wenzhou Medical University, Zhejiang, PR China.
authors.forename: Xiuling
authors.initials: X
authors.lastname: Wu
authors.affiliation: Department of Pathology, The First Affiliated Hospital of Wenzhou Medical University, Zhejiang, PR China.
authors.forename: Kate
authors.initials: K
authors.lastname: Huang
authors.affiliation: Department of B-Ultrasound, The First Affiliated Hospital of Wenzhou Medical University, Zhejiang, PR China.
authors.forename: Yuanping
authors.initials: Y
authors.lastname: Hu
authors.affiliation: Department of Medical Laboratory, The First Affiliated Hospital of Wenzhou Medical University, Zhejiang, PR China.
authors.forename: Xiangyang
authors.initials: X
authors.lastname: Lin
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D000951:Antigens, Neoplasm / Q000235:genetics*; D044466:Asian People / Q000235:genetics*; D001483:Base Sequence; D020022:Genetic Predisposition to Disease / Q000235:genetics*; D005838:Genotype; D006801:Humans; D008297:Male; D018895:Microsatellite Repeats; D008875:Middle Aged; D016017:Odds Ratio; D011401:Promoter Regions, Genetic / Q000235:genetics*; D011470:Prostatic Hyperplasia / Q000235:genetics; D011471:Prostatic Neoplasms / Q000235:genetics*; D062085:RNA, Long Noncoding / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D000951:Antigens, Neoplasm; D062085:RNA, Long Noncoding; C479341:prostate cancer antigen 3, human
keywords: Long noncoding RNA; Polymorphism; Prostate cancer; Prostate cancer antigen 3; Short tandem repeat