title: Methods of isolation and purification of outer membrane vesicles from gram-negative bacteria.
abstract: Outer membrane vesicles secreted by gram-negative bacteria play an important role in bacterial physiology as well as in virulence and host-pathogen interaction. Isolated vesicles of some bacteria have also been studied for their immunomodulatory potential in the vaccine development. However, the production of vesicles in sufficient amount, purity and reproducibility remains a critical challenge for subsequent analyses in most bacteria. In the present review methods of production, isolation, purification and quantification of outer membrane vesicles are summarized and discussed.
authors.affiliation: Department of Molecular Pathology and Biology, Faculty of Military Health Sciences, University of Defence, Trebesska 1575, 500 01 Hradec Kralove, Czech Republic. Electronic address: jana.klimentova@unob.cz.
authors.forename: Jana
authors.initials: J
authors.lastname: Klimentová
authors.affiliation: Department of Molecular Pathology and Biology, Faculty of Military Health Sciences, University of Defence, Trebesska 1575, 500 01 Hradec Kralove, Czech Republic. Electronic address: jiri.stulik@unob.cz.
authors.forename: Jiří
authors.initials: J
authors.lastname: Stulík
mesh_terms: D002458:Cell Fractionation / Q000379:methods*; D002473:Cell Wall / Q000276:immunology / Q000378:metabolism; D005818:Genetic Engineering; D006090:Gram-Negative Bacteria / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D022142:Secretory Vesicles / Q000276:immunology / Q000378:metabolism; D013312:Stress, Physiological; D013347:Subcellular Fractions
keywords: Bacterial secretion; Gram-negative bacteria; Host–pathogen interaction; Outer membrane vesicles; Vaccine development