title: Synthesis and characterization of pectin derivative with antitumor property against Caco-2 colon cancer cells.
abstract: New pectin derivative (Pec-MA) was obtained in specific reaction conditions. The presence of maleoyl groups in Pec-MA structure was confirmed by (1)H NMR and FTIR spectroscopy. The substitution degree of Pec-MA (DS=24%) was determined by (1)H NMR. The properties of Pec-MA were investigated through WAXS, TGA/DTG, SEM and zeta potential techniques. The Pec-MA presented amorphous characteristics and higher-thermal stability compared to raw pectin (Pec). In addition, considerable morphological differences between Pec-MA and Pec were observed by SEM. The cytotoxic effect on the Caco-2 cells showed that the Pec-MA significantly inhibited the growth of colon cancer cells whereas the Pec-MA does not show any cytotoxic effect on the VERO healthy cells. This result opens new perspectives for the manufacture of biomaterials based on Pec with anti-tumor properties.
authors.affiliation: Grupo de Materiais Poliméricos e Compósitos (GMPC), Av. Colombo, 5790, 87020-900 Maringá, Paraná, Brazil.
authors.forename: Elizângela A M S
authors.initials: EA
authors.lastname: Almeida
authors.affiliation: Universidade Tecnológica Federal do Paraná (UTFPR), Estrada para Boa Esperança, CEP 86400-000 Dois Vizinhos, Paraná, Brazil.
authors.forename: Suelen P
authors.initials: SP
authors.lastname: Facchi
authors.affiliation: Grupo de Materiais Poliméricos e Compósitos (GMPC), Av. Colombo, 5790, 87020-900 Maringá, Paraná, Brazil; Universidade Tecnológica Federal do Paraná (UTFPR), Estrada para Boa Esperança, CEP 86400-000 Dois Vizinhos, Paraná, Brazil. Electronic address: afmartins50@yahoo.com.br.
authors.forename: Alessandro F
authors.initials: AF
authors.lastname: Martins
authors.affiliation: Laboratório de Microbiologia Aplicada aos Produtos Naturais e Sintéticos, Av. Colombo, 5790, 87020-900, Maringá, Paraná, Brazil.
authors.forename: Samara
authors.initials: S
authors.lastname: Nocchi
authors.affiliation: Grupo de Materiais Poliméricos e Compósitos (GMPC), Av. Colombo, 5790, 87020-900 Maringá, Paraná, Brazil.
authors.forename: Ivânia T A
authors.initials: IT
authors.lastname: Schuquel
authors.affiliation: Laboratório de Microbiologia Aplicada aos Produtos Naturais e Sintéticos, Av. Colombo, 5790, 87020-900, Maringá, Paraná, Brazil.
authors.forename: Celso V
authors.initials: CV
authors.lastname: Nakamura
authors.affiliation: Grupo de Materiais Poliméricos e Compósitos (GMPC), Av. Colombo, 5790, 87020-900 Maringá, Paraná, Brazil.
authors.forename: Adley F
authors.initials: AF
authors.lastname: Rubira
authors.affiliation: Grupo de Materiais Poliméricos e Compósitos (GMPC), Av. Colombo, 5790, 87020-900 Maringá, Paraná, Brazil.
authors.forename: Edvani C
authors.initials: EC
authors.lastname: Muniz
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D018938:Caco-2 Cells; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002522:Chlorocebus aethiops; D003110:Colonic Neoplasms / Q000188:drug therapy / Q000473:pathology*; D004305:Dose-Response Relationship, Drug; D004354:Drug Screening Assays, Antitumor; D006801:Humans; D015394:Molecular Structure; D010368:Pectins / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D013329:Structure-Activity Relationship; D014709:Vero Cells
chemical_list: D000970:Antineoplastic Agents; D010368:Pectins
keywords: Antitumor property; Biopolymers; Caco-2 cells; Pectin; Pectin derivative; Vero cells