title: Fed-batch cultivation of Desmodesmus sp. in anaerobic digestion wastewater for improved nutrient removal and biodiesel production.
abstract: Desmodesmus sp. was used in anaerobically digested wastewater (ADW) for nutrients removal and the biodiesel production was measured and compared using fed-batch cultivation was investigated and compared with batch cultivation. The Desmodesmus sp. was able to remove 236.143, 268.238 and 6.427 mg/L of TN, NH4-N and PO4-P respectively after 40 d of fed-batch cultivation, while in batch cultivation the quantities of TN, NH4-N and PO4-P removed were 33.331, 37.227 and 1.323 mg/L. Biomass production of Desmodesmus sp. was also enhanced in fed-batch cultivation, when ADW loading was carried out every 2 days; the biomass concentration peaked at 1.039 g/L, which was three times higher than that obtained in batch cultivation (0.385 g/L). The highest lipid production (261.8 mg/L) was also recorded in fed-batch cultivation as compared to batch cultivation (83.3 mg/L). Fed-batch cultivation of Desmodesmus sp. could provide effective control of nutrients limitation and/or ammonia inhibition on microalgae cultivation.
authors.affiliation: College of Engineering/Biomass Engineering Center, China Agricultural University, Beijing 100083, China; Key Laboratory of Clean Production and Utilization of Renewable Energy, Ministry of Agriculture, P. R. China, Beijing 100083, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Ji
authors.affiliation: College of Engineering/Biomass Engineering Center, China Agricultural University, Beijing 100083, China; Key Laboratory of Clean Production and Utilization of Renewable Energy, Ministry of Agriculture, P. R. China, Beijing 100083, China. Electronic address: zhouyg@cau.edu.cn.
authors.forename: Yuguang
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: College of Engineering/Biomass Engineering Center, China Agricultural University, Beijing 100083, China; Key Laboratory of Clean Production and Utilization of Renewable Energy, Ministry of Agriculture, P. R. China, Beijing 100083, China.
authors.forename: Aiping
authors.initials: A
authors.lastname: Pang
authors.affiliation: College of Engineering/Biomass Engineering Center, China Agricultural University, Beijing 100083, China; Key Laboratory of Clean Production and Utilization of Renewable Energy, Ministry of Agriculture, P. R. China, Beijing 100083, China.
authors.forename: Li
authors.initials: L
authors.lastname: Ning
authors.affiliation: College of Engineering/Biomass Engineering Center, China Agricultural University, Beijing 100083, China; Key Laboratory of Clean Production and Utilization of Renewable Energy, Ministry of Agriculture, P. R. China, Beijing 100083, China.
authors.forename: Kibet
authors.initials: K
authors.lastname: Rodgers
authors.affiliation: College of Agriculture and Biotechnology, China Agricultural University, Beijing 100193, China; Key Laboratory of Clean Production and Utilization of Renewable Energy, Ministry of Agriculture, P. R. China, Beijing 100083, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Liu
authors.affiliation: College of Engineering/Biomass Engineering Center, China Agricultural University, Beijing 100083, China; Key Laboratory of Clean Production and Utilization of Renewable Energy, Ministry of Agriculture, P. R. China, Beijing 100083, China.
authors.forename: Renjie
authors.initials: R
authors.lastname: Dong
mesh_terms: D000693:Anaerobiosis; D061249:Batch Cell Culture Techniques / Q000379:methods*; D056804:Biofuels; D018533:Biomass; D000460:Chlorophyta / Q000254:growth & development*; D004952:Esters / Q000378:metabolism; D005227:Fatty Acids / Q000378:metabolism; D008055:Lipids / Q000096:biosynthesis; D009584:Nitrogen / Q000302:isolation & purification*; D010743:Phospholipids / Q000378:metabolism; D010758:Phosphorus / Q000302:isolation & purification*; D014280:Triglycerides / Q000378:metabolism; D062065:Wastewater
chemical_list: D056804:Biofuels; D004952:Esters; D005227:Fatty Acids; D008055:Lipids; D009584:Nitrogen; D010743:Phospholipids; D010758:Phosphorus; D014280:Triglycerides; D062065:Wastewater
keywords: Anaerobic digestion wastewater (ADW); Biomass and lipid production; Desmodesmus sp.; Fed-batch cultivation; Nutrients removal