title: Biomarker research for moyamoya disease in cerebrospinal fluid using surface-enhanced laser desorption/ionization time-of-flight mass spectrometry.
abstract: Moyamoya disease (MMD) is a rare cerebrovascular disease characterized by steno-occlusive change in bilateral internal carotid arteries with unknown etiology. To discover biomarker candidates in cerebrospinal fluid from MMD patients, proteome analysis was performed by the surface-enhanced laser desorption/ionization time-of-flight mass spectrometry. Three peptides, 4473Da, 4475Da, and 6253Da, were significantly elevated in MMD group. A positive correlation between 4473Da peptide and postoperative angiogenesis was determined. Twenty MMD patients were enrolled in this pilot study, including 11 pediatric cases less than 18 years of age (mean age, 8.67 years) and 9 adult MMD patients (mean age, 38.1 years). This study also includes 17 control cases with the mean age of 27.9 years old. In conclusion, 4473Da peptide is supposed to be a reliable biomarker of MMD. 4473Da peptide showed higher intensity peaks especially in younger MMD patients, and it was proved to be highly related to postoperative angiogenesis. Further study is needed to show how 4473Da peptide is involved with the etiology and the onset of MMD.
authors.affiliation: Department of Neurosurgery, Nagoya University Graduate School of Medicine, Nagoya, Japan. Electronic address: maruwakamikio@gmail.com.
authors.forename: Mikio
authors.initials: M
authors.lastname: Maruwaka
authors.affiliation: Division of Advanced Research Promotion Institute of Comprehensive Medical Research, Nagakute-City, Japan; Division of Advanced Medicine Advanced Medical Research Center; Laboratory of Cell Therapy Division of Advanced Medical Research Center.
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Yoshikawa
authors.affiliation: Department of Neurosurgery, Nagoya University Graduate School of Medicine, Nagoya, Japan.
authors.forename: Sho
authors.initials: S
authors.lastname: Okamoto
authors.affiliation: Department of Neurosurgery, Nagoya University Graduate School of Medicine, Nagoya, Japan.
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Araki
authors.affiliation: Department of Neurosurgery, Komaki Municipal Hospital, Komaki-City, Japan.
authors.forename: Masaki
authors.initials: M
authors.lastname: Sumitomo
authors.affiliation: Department of Neurosurgery, Nagoya University Graduate School of Medicine, Nagoya, Japan.
authors.forename: Akino
authors.initials: A
authors.lastname: Kawamura
authors.affiliation: Department of Neurosurgery, Nagoya University Graduate School of Medicine, Nagoya, Japan.
authors.forename: Kinya
authors.initials: K
authors.lastname: Yokoyama
authors.affiliation: Department of Neurosurgery, Nagoya University Graduate School of Medicine, Nagoya, Japan.
authors.forename: Toshihiko
authors.initials: T
authors.lastname: Wakabayashi
mesh_terms: D000293:Adolescent; D000328:Adult; D015415:Biomarkers / Q000134:cerebrospinal fluid*; D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D007223:Infant; D008279:Magnetic Resonance Imaging; D008297:Male; D008875:Middle Aged; D009072:Moyamoya Disease / Q000134:cerebrospinal fluid* / Q000473:pathology; D009389:Neovascularization, Pathologic / Q000473:pathology; D010865:Pilot Projects; D040901:Proteomics / Q000379:methods; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D055815:Young Adult
chemical_list: D015415:Biomarkers
keywords: Moyamoya disease (MMD); angiogenesis; biomarker; cerebrospinal fluid (CSF); surface-enhanced laser desorption/ionization mass spectrometry (SELDI-TOF-MS)