title: Apoptosis in liver cancer (HepG2) cells induced by functionalized gold nanoparticles.
abstract: An ethnopharmacological approach for biosynthesis of gold nanoparticles is being demonstrated using seed coat of Cajanus cajan. Medicinal value of capping molecule investigated for anticancer activity and results disclose its greater potential. The active principle of the seed coat [3-butoxy-2-hydroxypropyl 2-(2,4-dihydroxyphenyl) acetate] is elucidated. Rapid one-step synthesis yields highly stable, monodisperse (spherical) gold nanoparticles in the size ranging from 9 to 41 nm. Anticancer activity has been studied using liver cancer cells and cytotoxic mechanism has been evaluated using MTT, Annexin-V/PI Double-Staining Assay, Cell cycle, Comet assay and Flow cytometric analysis for apoptosis. The present investigation will open up a new possibility of functionalizing gold nanoparticles for apoptosis studies in liver cancer cells.
authors.affiliation: Nanoscience Division, Department of Zoology, Thiruvalluvar University, Vellore 632 115, Tamilnadu, India.
authors.forename: Thirunavukkarasu
authors.initials: T
authors.lastname: Ashokkumar
authors.affiliation: Department of Zoology, University of Madras, Guindy Campus, Chennai 600 025, Tamilnadu, India.
authors.forename: Durai
authors.initials: D
authors.lastname: Prabhu
authors.affiliation: Nanoscience Division, Department of Zoology, Thiruvalluvar University, Vellore 632 115, Tamilnadu, India.
authors.forename: Ravi
authors.initials: R
authors.lastname: Geetha
authors.affiliation: Centre for Ocean Research, Sathyabama University, Chennai 600119, Tamilnadu, India.
authors.forename: Kasivelu
authors.initials: K
authors.lastname: Govindaraju
authors.affiliation: Department of Zoology, University of Madras, Guindy Campus, Chennai 600 025, Tamilnadu, India.
authors.forename: Ramar
authors.initials: R
authors.lastname: Manikandan
authors.affiliation: Department of Zoology, University of Madras, Guindy Campus, Chennai 600 025, Tamilnadu, India.
authors.forename: Chinnasamy
authors.initials: C
authors.lastname: Arulvasu
authors.affiliation: Nanoscience Division, Department of Zoology, Thiruvalluvar University, Vellore 632 115, Tamilnadu, India. Electronic address: gsvelu@gmail.com.
authors.forename: Ganesan
authors.initials: G
authors.lastname: Singaravelu
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D006046:Gold / Q000737:chemistry*; D056945:Hep G2 Cells; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry*
chemical_list: D006046:Gold
keywords: Apoptosis; Cajanus cajan; Gold nanoparticles; Liver cancer; in vitro