title: Prevention of degradation of the natural high potency sweetener (2R,4R)-monatin in mock beverage solutions.
abstract: Exposure of the naturally-occurring sweetener monatin to light and metal ions results in loss of both parent monatin and total indole (monatin plus monatin lactone/lactam) in mock beverage solutions, with an accompanying decrease in sweetness. In this study potential protective strategies to prevent degradation were investigated. Metal ion chelating resin, or the chelators EDTA and desferrioxamine decreased monatin and indole loss for solutions kept either in darkness or exposed to light. Tannic acid and Chinese bayberry extract both afforded protection, but this did not arise from a light filtering effect. Plastics with defined absorbance characteristics provided protection with this being wavelength dependent; yellow transparent PET plastic was most effective. The contribution of these interventions (metal ion removal/binding; antioxidant; light absorption) was additive, with combinations providing the greatest protective effect against monatin and indole loss. These results indicate that it is possible to minimise monatin degradation by appropriate choices of treatments, additives and container.
authors.affiliation: The Heart Research Institute, 7 Eliza Street, Newtown, NSW 2042, Australia; Faculty of Medicine, University of Sydney, Sydney, NSW 2006, Australia.
authors.forename: Corin
authors.initials: C
authors.lastname: Storkey
authors.affiliation: The Heart Research Institute, 7 Eliza Street, Newtown, NSW 2042, Australia; Faculty of Medicine, University of Sydney, Sydney, NSW 2006, Australia.
authors.forename: David I
authors.initials: DI
authors.lastname: Pattison
authors.affiliation: Cargill, Inc., 15407 McGinty Road West, Wayzata, MN 55391, USA.
authors.forename: Jamie A
authors.initials: JA
authors.lastname: Koehler
authors.affiliation: Cargill, Inc., 15407 McGinty Road West, Wayzata, MN 55391, USA.
authors.forename: Dan S
authors.initials: DS
authors.lastname: Gaspard
authors.affiliation: Cargill, Inc., 15407 McGinty Road West, Wayzata, MN 55391, USA.
authors.forename: Jeff C
authors.initials: JC
authors.lastname: Evans
authors.affiliation: Cargill, Inc., 15407 McGinty Road West, Wayzata, MN 55391, USA.
authors.forename: Erik D
authors.initials: ED
authors.lastname: Hagestuen
authors.affiliation: The Heart Research Institute, 7 Eliza Street, Newtown, NSW 2042, Australia; Faculty of Medicine, University of Sydney, Sydney, NSW 2006, Australia. Electronic address: davies@sund.ku.dk.
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Davies
mesh_terms: D000975:Antioxidants / Q000494:pharmacology; D001628:Beverages / Q000032:analysis*; D002614:Chelating Agents / Q000494:pharmacology; D004492:Edetic Acid / Q000494:pharmacology; D018698:Glutamic Acid / Q000031:analogs & derivatives* / Q000737:chemistry; D007211:Indoles / Q000737:chemistry*; D008027:Light; D013549:Sweetening Agents / Q000737:chemistry*
chemical_list: C414924:4-hydroxy-4-(indol-3-ylmethyl)glutamic acid; D000975:Antioxidants; D002614:Chelating Agents; D007211:Indoles; D013549:Sweetening Agents; D018698:Glutamic Acid; D004492:Edetic Acid
keywords: Metal ions; Monatin; Photo-oxidation; Radicals; Singlet oxygen; Sweetener; Tryptophan