title: Effects of pressure on the shear modulus, mass and thickness of the perfused porcine kidney.
abstract: Eleven fresh ex vivo porcine kidneys were perfused in the artery, vein and ureter with degassed Dulbecco׳s Modified Eagle Medium (DMEM). The effect of perfusion pressure was evaluated using ten different pressures combinations. The shear modulus of the tissues was estimated during perfusion using shear wave elastography. The organ weight change was measured by a digital scale and cameras were used to follow the changes of the dimensions after each pressure combination. The effect of perfusion on the weight and the thickness was non-reversible, whereas the effect on the shear modulus was reversible. Pressure was found to increase the average shear modulus in the cortex by as much as 73%. A pressure of 80 mmHg was needed to observe tissues shear modulus in the same range as in vivo tests (Gcortex=9.1 kPa, Gmedulla=8.5 kPa ex vivo versus Gcortex=9.1 kPa, Gmedulla=8.7 kPa in vivo in Gennisson et al., 2012).
authors.affiliation: Université de Lyon, F-69622, Lyon, France; IFSTTAR, LBMC, UMR_T9406, F-69675, Bron, France; Université Lyon 1, F-69622, Villeurbanne, France.
authors.forename: C
authors.initials: C
authors.lastname: Helfenstein
authors.affiliation: Institut Langevin - Ondes et Images, ESPCI ParisTech, CNRS UMR7587, INSERM U979, ESPCI, 1 rue Jussieu, 75005 Paris, France.
authors.forename: J-L
authors.initials: JL
authors.lastname: Gennisson
authors.affiliation: Institut Langevin - Ondes et Images, ESPCI ParisTech, CNRS UMR7587, INSERM U979, ESPCI, 1 rue Jussieu, 75005 Paris, France.
authors.forename: M
authors.initials: M
authors.lastname: Tanter
authors.affiliation: Université de Lyon, F-69622, Lyon, France; IFSTTAR, LBMC, UMR_T9406, F-69675, Bron, France; Université Lyon 1, F-69622, Villeurbanne, France. Electronic address: Philippe.beillas@ifsttar.fr.
authors.forename: P
authors.initials: P
authors.lastname: Beillas
mesh_terms: D000818:Animals; D001696:Biomechanical Phenomena / Q000502:physiology; D055119:Elastic Modulus / Q000502:physiology*; D054459:Elasticity Imaging Techniques; D007668:Kidney / Q000033:anatomy & histology* / Q000098:blood supply / Q000502:physiology*; D023421:Models, Animal; D009929:Organ Size / Q000502:physiology; D010477:Perfusion / Q000379:methods; D011312:Pressure / Q000009:adverse effects*; D033081:Shear Strength / Q000502:physiology*; D013314:Stress, Mechanical; D013552:Swine
keywords: Elastography; Kidney; Pressure; Shear modulus