title: The synthesis of sulforaphane analogues and their protection effect against cisplatin induced cytotoxicity in kidney cells.
abstract: A series of sulforaphane analogues were synthesized with various amines by treatment of carbon disulfide followed by Boc₂O and DMAP. These synthesized sulforaphane analogues were tested on cisplatin treated cultured LLC-PK1 kidney cell line. Among these analogues, several compounds including SF5 show a potent effect on kidney cell protection assay at the concentration of 2.5 μM. Further studies with compound SF5 revealed that the kidney cell protection effect was related by inhibiting the apoptosis pathway through JNK-p53-caspase apoptotic cascade. Compound SF5 may be considered as a promising candidate for the development of new kidney protection agent against drug induced acute kidney disease.
authors.affiliation: Natural Product Research Center, Korea Institute of Science and Technology, Gangneung 210-340, Republic of Korea.
authors.forename: Taejung
authors.initials: T
authors.lastname: Kim
authors.forename: Young-Joo
authors.initials: YJ
authors.lastname: Kim
authors.forename: Im-Ho
authors.initials: IH
authors.lastname: Han
authors.forename: Dahae
authors.initials: D
authors.lastname: Lee
authors.forename: Jungyeob
authors.initials: J
authors.lastname: Ham
authors.forename: Ki Sung
authors.initials: KS
authors.lastname: Kang
authors.forename: Jae Wook
authors.initials: JW
authors.lastname: Lee
mesh_terms: D000818:Animals; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D002945:Cisplatin / Q000037:antagonists & inhibitors / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D017879:Isothiocyanates / Q000138:chemical synthesis* / Q000494:pharmacology; D007668:Kidney / Q000187:drug effects* / Q000502:physiology; D018374:LLC-PK1 Cells; D020011:Protective Agents / Q000138:chemical synthesis* / Q000494:pharmacology; D013454:Sulfoxides; D013552:Swine
chemical_list: D017879:Isothiocyanates; D020011:Protective Agents; D013454:Sulfoxides; C016766:sulforaphane; D002945:Cisplatin