title: A new method for polyhydroxyalkanoate (PHA) accumulating bacteria selection under physical selective pressure.
abstract: An aerobic dynamic discharge (ADD) process involving physical selective pressure to favor the accumulation of polyhydroxyalkanoates (PHAs) accumulating bacteria in microbial mixed cultures (MMCs) is proposed and evaluated in this study. Sequencing batch reactor PHA production in the ADD mode was compared to the conventional aerobic dynamic feeding (ADF) mode. Results showed that the cultures selected under the ADD mode showed a better PHA producing potential than ADF mode cultures in terms of both maximum PHA content and selection time, explained by altered physical selective pressure. The maximum PHA content of 74.16 (± 0.03)% and PHA yield of 0.72 (± 0.07) C mol PHA/C mol Ac were achieved after 30 days of the ADD operation, which were much higher than those obtained under the same organic loading rate in the ADF operation. The ADD mode is a promising method for quick selection of a strong-PHA accumulating culture.
authors.affiliation: State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology (SKLUWRE, HIT), Harbin 150090, China.
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology (SKLUWRE, HIT), Harbin 150090, China.
authors.forename: Zirui
authors.initials: Z
authors.lastname: Guo
authors.affiliation: State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology (SKLUWRE, HIT), Harbin 150090, China. Electronic address: wqxshelly@163.com.
authors.forename: Qinxue
authors.initials: Q
authors.lastname: Wen
authors.affiliation: State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology (SKLUWRE, HIT), Harbin 150090, China.
authors.forename: Long
authors.initials: L
authors.lastname: Huang
authors.affiliation: Faculty of Technology, Telemark University College, Porsgrunn 3901, Norway.
authors.forename: Rune
authors.initials: R
authors.lastname: Bakke
authors.affiliation: State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology (SKLUWRE, HIT), Harbin 150090, China.
authors.forename: Maoan
authors.initials: M
authors.lastname: Du
mesh_terms: D001419:Bacteria / Q000302:isolation & purification*; D061249:Batch Cell Culture Techniques; D019149:Bioreactors / Q000382:microbiology; D001709:Biotechnology / Q000379:methods*; D007700:Kinetics; D054813:Polyhydroxyalkanoates / Q000378:metabolism*; D011312:Pressure; D013997:Time Factors
chemical_list: D054813:Polyhydroxyalkanoates
keywords: Aerobic dynamic discharge (ADD); Aerobic dynamic feeding (ADF); Mixed microbial culture (MMC)