title: Aminocella lysinolytica gen. nov., sp. nov., a L-lysine-degrading, strictly anaerobic bacterium in the class Clostridia isolated from a methanogenic reactor of cattle farms.
abstract: A strictly anaerobic bacterial strain (WN037(T)) was isolated from a methanogenic reactor. Cells were Gram-positive rods. Strain WN037(T) was asaccharolytic. The strain fermented L-lysine in the presence of B-vitamin mixture or vitamin B12 and produced acetate and butyrate. L-arginine and casamino acids poorly supported the growth. Strain WN037(T) used neither other amino acids nor organic acids examined. The strain had C18:1 ω7c, C16:0 and C18:1 ω7c DMA as the predominant cellular fatty acids. The genomic DNA G + C content was 44.2 mol %. Phylogenetic analysis based on the 16S rRNA gene sequence placed strain WN037(T) in the family Eubacteriaceae in the class Clostridia. The closest relative was Eubacterium pyruvativorans (sequence similarity, 92.8 %). Based on the comprehensive analyses, the novel genus and species, Aminocella lysinolytica gen. nov., sp. nov. was proposed to accommodate the strain. The type strain is WN037(T) (= JCM 19863(T) = DSM 28287(T)).
authors.affiliation: Faculty of Agriculture, Yamagata University, Wakaba-machi 1-23, Tsuruoka, 997-8555, Japan, uatsuko@tds1.tr.yamagata-u.ac.jp.
authors.forename: Atsuko
authors.initials: A
authors.lastname: Ueki
authors.forename: Toru
authors.initials: T
authors.lastname: Shibuya
authors.forename: Nobuo
authors.initials: N
authors.lastname: Kaku
authors.forename: Katsuji
authors.initials: K
authors.lastname: Ueki
mesh_terms: D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D001421:Bacteria, Anaerobic / Q000145:classification* / Q000166:cytology / Q000302:isolation & purification* / Q000502:physiology; D001482:Base Composition; D019149:Bioreactors; D002417:Cattle; D003470:Culture Media; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D005285:Fermentation; D020459:Genes, rRNA; D006094:Gram-Positive Bacteria / Q000145:classification* / Q000166:cytology / Q000302:isolation & purification* / Q000502:physiology; D008239:Lysine / Q000378:metabolism*; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D000596:Amino Acids; D003470:Culture Media; D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D008239:Lysine