title: The effects of Ficus carica polysaccharide on immune response and expression of some immune-related genes in grass carp, Ctenopharyngodon idella.
abstract: The present study investigated the effect of Ficus carica polysaccharide (FCP), isolated from the fruit of F. carica L., at 0%, 0.1%, 0.5% and 1.0% doses supplementation with feed on genes Interleukin 1-β (IL-1β), Tumor Necrosis Factor α (TNF-α) and heat shock protein 70 (HSP70) gene expression in blood, humoral innate immune parameters and resistant to Flavobacterium columnare of grass carp at weeks 1, 2 and 3. The results revealed that administration of FCP significantly (P<0.05) up regulated IL-1β and TNF-α gene expression. HSP70 gene expression was significantly (P<0.05) lower in FCP-fed fish at the end of trial. The serum total protein, albumin and globulin did not significantly increased in any diet on the first week whereas it was significantly enhanced in 0.5% and 1.0% supplementation diets on weeks 2 and 3 when compared to control. The serum complement C3 was significantly (P<0.05) increased on weeks 1 and 2 when compared to control, however, no significant difference was found in this activity after 3 weeks of treatment. All diets significantly enhanced the serum lysozyme activity, bactericidal activity from weeks 1-2 as compared to control. Grass carp fed with FCP showed remarkably higher resistance against F. columnare (60% survival) compared to the control group (30% survival). These results confirm that FCP can up regulate immune related genes expression, stimulates immune response that per se enhances disease resistance in grass carp.
authors.affiliation: Zhejiang Provincial Key Laboratory of Aquatic Resources Conservation and Development, College of Life Science, Huzhou University, Huzhou, Zhejiang, 313001, China.
authors.forename: Xia
authors.initials: X
authors.lastname: Yang
authors.affiliation: Zhejiang Institute of Freshwater Fisheries, Huzhou, Zhejiang, 313001, China.
authors.forename: Jian Lin
authors.initials: JL
authors.lastname: Guo
authors.affiliation: Zhejiang Provincial Key Laboratory of Aquatic Resources Conservation and Development, College of Life Science, Huzhou University, Huzhou, Zhejiang, 313001, China. Electronic address: yjy@hutc.zj.cn.
authors.forename: Jin Yun
authors.initials: JY
authors.lastname: Ye
authors.affiliation: Zhejiang Provincial Key Laboratory of Aquatic Resources Conservation and Development, College of Life Science, Huzhou University, Huzhou, Zhejiang, 313001, China.
authors.forename: Yi Xiang
authors.initials: YX
authors.lastname: Zhang
authors.affiliation: Zhejiang Provincial Key Laboratory of Aquatic Resources Conservation and Development, College of Life Science, Huzhou University, Huzhou, Zhejiang, 313001, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
mesh_terms: D000818:Animals; D017756:Aquaculture / Q000379:methods; D001798:Blood Proteins / Q000378:metabolism; D002347:Carps; D003176:Complement C3 / Q000276:immunology; D017931:DNA Primers / Q000235:genetics; D004305:Dose-Response Relationship, Drug; D030681:Ficus / Q000737:chemistry*; D005393:Fish Diseases / Q000276:immunology* / Q000382:microbiology*; D045826:Flavobacteriaceae Infections / Q000276:immunology / Q000662:veterinary*; D005417:Flavobacterium / Q000187:drug effects*; D005786:Gene Expression Regulation / Q000187:drug effects / Q000276:immunology; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism; D056724:Immunity, Humoral / Q000187:drug effects; D008516:Medicine, Chinese Traditional; D011134:Polysaccharides / Q000032:analysis / Q000494:pharmacology*; D018709:Statistics, Nonparametric; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D001798:Blood Proteins; D003176:Complement C3; D017931:DNA Primers; D018840:HSP70 Heat-Shock Proteins; D011134:Polysaccharides; D014409:Tumor Necrosis Factor-alpha
keywords: Ficus carica polysaccharide; Grass carp; Humoral innate immune; Immune-related genes