title: Electrochemical biosensor based on glucose oxidase encapsulated within enzymatically synthesized poly(1,10-phenanthroline-5,6-dione).
abstract: This study is focused on the investigation of electrocatalytic effect of glucose oxidase (GOx) immobilized on the graphite rod (GR) electrode. The enzyme modified electrode was prepared by encapsulation of immobilized GOx within enzymatically formed poly(1,10-phenanthroline-5,6-dione) (pPD) film. The electrochemical responses of such enzymatic electrode (pPD/GOx/GR) vs. different glucose concentrations were examined chronoamperometrically in acetate-phosphate buffer solution (A-PBS), pH 6.0, under aerobic or anaerobic conditions. Amperometric signals of the pPD/GOx/GR electrode exhibited well-defined hyperbolic dependence upon glucose concentration. Amperometric signals at 100mM of glucose were 41.17 and 32.27 μA under aerobic and anaerobic conditions, respectively. Amperometric signals of the pPD/GOx/GR electrode decreased by 6% within seven days. The pPD/GOx/GR electrode showed excellent selectivity in the presence of dopamine and uric acid. Furthermore it had a good reproducibility and repeatability with standard deviation of 9.4% and 8.0%, respectively.
authors.affiliation: Kirikkale University, Kirikkale Vocational High School, Department of Chemistry and Chemical Processing Technologies, Kirikkale, Turkey; Vilnius University, Faculty of Chemistry, NanoTechnas - Centre of Nanotechnology and Materials Science and Department of Physical Chemistry, Vilnius, Lithuania; State Research Institute, Center for Physical Sciences and Technology, Laboratory of Bio-nanotechnology, Vilnius Lithuania.
authors.forename: Hakan
authors.initials: H
authors.lastname: Ciftci
authors.affiliation: Vilnius University, Faculty of Chemistry, NanoTechnas - Centre of Nanotechnology and Materials Science and Department of Physical Chemistry, Vilnius, Lithuania; State Research Institute, Center for Physical Sciences and Technology, Laboratory of Bio-nanotechnology, Vilnius Lithuania; Selcuk University, Faculty of Science, Department of Chemistry, Konya, Turkey.
authors.forename: Yasemin
authors.initials: Y
authors.lastname: Oztekin
authors.affiliation: Gazi University, Faculty of Pharmacy, Department of Analytical Chemistry, Ankara, Turkey.
authors.forename: Ugur
authors.initials: U
authors.lastname: Tamer
authors.affiliation: Vilnius University, Faculty of Chemistry, NanoTechnas - Centre of Nanotechnology and Materials Science and Department of Physical Chemistry, Vilnius, Lithuania.
authors.forename: Almira
authors.initials: A
authors.lastname: Ramanaviciene
authors.affiliation: Vilnius University, Faculty of Chemistry, NanoTechnas - Centre of Nanotechnology and Materials Science and Department of Physical Chemistry, Vilnius, Lithuania; State Research Institute, Center for Physical Sciences and Technology, Laboratory of Bio-nanotechnology, Vilnius Lithuania. Electronic address: arunas.ramanavicius@chf.vu.lt.
authors.forename: Arunas
authors.initials: A
authors.lastname: Ramanavicius
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D004298:Dopamine / Q000378:metabolism; D004563:Electrochemistry / Q000379:methods*; D004800:Enzymes, Immobilized / Q000737:chemistry* / Q000378:metabolism*; D005947:Glucose / Q000032:analysis*; D005949:Glucose Oxidase / Q000737:chemistry* / Q000378:metabolism*; D010618:Phenanthrolines / Q000378:metabolism*; D014527:Uric Acid / Q000378:metabolism
chemical_list: D004800:Enzymes, Immobilized; D010618:Phenanthrolines; D014527:Uric Acid; C037153:1,10-phenanthroline-5,6-dione; D005949:Glucose Oxidase; D005947:Glucose; D004298:Dopamine
keywords: 1,10-Phenanthroline-5,6-dione; Electrocatalysis; Enzymatic polymerization; Glucose biosensor; Glucose oxidase