title: Oligosaccharidic fractions derived from Triticum vulgare extract accelerate tissutal repairing processes in in vitro and in vivo models of skin lesions.
abstract: BACKGROUND
  Triticum vulgare has been extensively used in traditional medicine thanks to its properties of accelerating tissue repair. The aqueous extract of Triticum vulgare (TVE) is currently an active component used by Farmaceutici Damor in the manufacture of certain pharmaceutical products already marketed in Italy and abroad under the brand name Fitostimoline(®), in the formulation of cream and medicated gauze and is commonly used for the treatment of decubitus ulcers, sores, burns, scarring delays, dystrophic diseases, and, more broadly, in the presence of problems relating to re-epithelialization or tissue regeneration. The active components of Fitostimoline(®)-based products determine a marked acceleration of tissutal repairing processes, stimulate chemotaxis and the fibroblastic maturation, and significantly increase the fibroblastic index, which are crucial points in the repairing processes. The aim of the present paper was to identify and characterize the active fractions of TVE responsible for the pharmacological effect in tissutal repairing processes.
  
  
  METHODS
  Several fractions obtained from TVE by ultrafiltration procedures and HPAE chromatography were tested to measure their growth-enhancing activity on NIH-3T3 fibroblasts. The healing action of the same fractions, prepared as cream formulation, was assessed in rat subjected to two different models of skin lesion, skin scarification and excision.
  
  
  RESULTS
  Our results showed a pro-proliferative effect of the fractions ST-98 and K>1000 in NIH-3T3 fibroblasts. Moreover these fractions formulated as cream preparations were effective also in in vivo models of skin lesion.
  
  
  CONCLUSIONS
  The results of the present study showed that these active fractions of TVE are responsible for its pro-proliferative effect.
authors.affiliation: Department of Molecular Medicine and Medical Biotechnology, School of Medicine, Federico II University of Naples, Via Pansini 5, 80131 Naples, Italy.
authors.forename: Luca
authors.initials: L
authors.lastname: Sanguigno
authors.affiliation: Farmaceutici Damor S.p.A, Via E. Scaglione 27, 80145 Naples, Italy.
authors.forename: Massimiliano
authors.initials: M
authors.lastname: Minale
authors.affiliation: Farmaceutici Damor S.p.A, Via E. Scaglione 27, 80145 Naples, Italy.
authors.forename: Ernesto
authors.initials: E
authors.lastname: Vannini
authors.affiliation: Farmaceutici Damor S.p.A, Via E. Scaglione 27, 80145 Naples, Italy.
authors.forename: Guido
authors.initials: G
authors.lastname: Arato
authors.affiliation: Farmaceutici Damor S.p.A, Via E. Scaglione 27, 80145 Naples, Italy.
authors.forename: Rodolfo
authors.initials: R
authors.lastname: Riccio
authors.affiliation: Department of Pharmaceutical Sciences, School of Pharmacy, University of Salerno, Via Don Melillo Ponte, 84084 Fisciano Salerno, Italy.
authors.forename: Agostino
authors.initials: A
authors.lastname: Casapullo
authors.affiliation: Department of Pharmaceutical Sciences, School of Pharmacy, University of Salerno, Via Don Melillo Ponte, 84084 Fisciano Salerno, Italy.
authors.forename: Maria Chiara
authors.initials: MC
authors.lastname: Monti
authors.affiliation: Department of Pharmaceutical Sciences, School of Pharmacy, University of Salerno, Via Don Melillo Ponte, 84084 Fisciano Salerno, Italy.
authors.forename: Raffaele
authors.initials: R
authors.lastname: Riccio
authors.affiliation: Department of Molecular Medicine and Medical Biotechnology, School of Medicine, Federico II University of Naples, Via Pansini 5, 80131 Naples, Italy.
authors.forename: Silvestro
authors.initials: S
authors.lastname: Formisano
authors.affiliation: Division of Pharmacology, Department of Neuroscience, Reproductive and Odontostomatological Sciences, School of Medicine, Federico II University of Naples, Via Pansini 5, 80131 Naples, Italy.
authors.forename: Gianfranco
authors.initials: G
authors.lastname: Di Renzo
authors.affiliation: Division of Pharmacology, Department of Neuroscience, Reproductive and Odontostomatological Sciences, School of Medicine, Federico II University of Naples, Via Pansini 5, 80131 Naples, Italy. Electronic address: orcuomo@yahoo.it.
authors.forename: Ornella
authors.initials: O
authors.lastname: Cuomo
mesh_terms: D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D008297:Male; D051379:Mice; D041681:NIH 3T3 Cells; D009844:Oligosaccharides / Q000032:analysis / Q000494:pharmacology* / Q000627:therapeutic use*; D008517:Phytotherapy; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use*; D017207:Rats, Sprague-Dawley; D012871:Skin Diseases / Q000188:drug therapy; D014908:Triticum; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D009844:Oligosaccharides; D010936:Plant Extracts
keywords: Phytostimolin; Skin lesion; Tissue repair; Triticum vulgare