title: Stability and nitrite-oxidizing bacteria community structure in different high-rate CANON reactors.
abstract: In completely autotrophic nitrogen removal over nitrite (CANON) process, the bioactivity of nitrite-oxidizing bacteria (NOB) should be effectively inhibited. In this study, the stability of four high-rate CANON reactors and the effect of free ammonia (FA) and organic material on NOB community structure were investigated using DGGE. Results suggested that with the increasing of FA, the ratio of total nitrogen removal to nitrate production went up gradually, while the biodiversity of Nitrobacter-like NOB and Nitrospira-like NOB both decreased. When the CANON reactor was transformed to simultaneous partial nitrification, anammox and denitrification (SNAD) reactor by introducing organic material, the denitrifiers and aerobic heterotrophic bacteria would compete nitrite or oxygen with NOB, which then led to the biodiversity decreasing of both Nitrobacter-like NOB and Nitrospira-like NOB. The distribution of Nitrobacter-like NOB and Nitrospira-like NOB were evaluated, and finally effective strategies for suppressing NOB in CANON reactors were proposed.
authors.affiliation: Key Laboratory of Water Quality Science and Water Environment Recovery Engineering, Beijing University of Technology, Beijing 100124, China.
authors.forename: Yuhai
authors.initials: Y
authors.lastname: Liang
authors.affiliation: Key Laboratory of Water Quality Science and Water Environment Recovery Engineering, Beijing University of Technology, Beijing 100124, China. Electronic address: lidong2006@bjut.edu.cn.
authors.forename: Dong
authors.initials: D
authors.lastname: Li
authors.affiliation: Henan Collaborative Innovation Center of Environmental Pollution Control and Ecological Restoration, Zhengzhou University of Light Industry, Zhengzhou 450001, China.
authors.forename: Xiaojing
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Water Quality Science and Water Environment Recovery Engineering, Beijing University of Technology, Beijing 100124, China.
authors.forename: Huiping
authors.initials: H
authors.lastname: Zeng
authors.affiliation: Key Laboratory of Water Quality Science and Water Environment Recovery Engineering, Beijing University of Technology, Beijing 100124, China.
authors.forename: Zhuo
authors.initials: Z
authors.lastname: Yang
authors.affiliation: Key Laboratory of Water Quality Science and Water Environment Recovery Engineering, Beijing University of Technology, Beijing 100124, China.
authors.forename: Shaoming
authors.initials: S
authors.lastname: Cui
authors.affiliation: Key Laboratory of Water Quality Science and Water Environment Recovery Engineering, Beijing University of Technology, Beijing 100124, China; State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Zhang
mesh_terms: D000641:Ammonia / Q000378:metabolism; D052818:Autotrophic Processes; D001419:Bacteria / Q000235:genetics / Q000378:metabolism*; D044822:Biodiversity; D019149:Bioreactors / Q000382:microbiology*; D058645:Denaturing Gradient Gel Electrophoresis / Q000379:methods; D058440:Denitrification; D005227:Fatty Acids / Q000378:metabolism; D059013:Microbial Consortia / Q000235:genetics / Q000502:physiology*; D058465:Nitrification; D009573:Nitrites / Q000378:metabolism*; D009576:Nitrobacter / Q000378:metabolism; D009584:Nitrogen / Q000378:metabolism; D010084:Oxidation-Reduction; D010100:Oxygen / Q000378:metabolism; D016133:Polymerase Chain Reaction / Q000379:methods; D014865:Waste Disposal, Fluid / Q000295:instrumentation*
chemical_list: D005227:Fatty Acids; D009573:Nitrites; D000641:Ammonia; D009584:Nitrogen; D010100:Oxygen
keywords: CANON; DGGE; Nitrobacter; Nitrospira; SNAD