title: Bacterial community variation and microbial mechanism of triclosan (TCS) removal by constructed wetlands with different types of plants.
abstract: Triclosan (TCS) is a broad-spectrum synthetic antimicrobial agent that is toxic to microbes and other aquatic organisms. Constructed wetlands (CWs) are now popular in TCS removal. However, knowledge on the effects of TCS on the bacterial community and microbial removal mechanism in CWs is lacking. The effects of TCS (60 μg L(-1)) on bacterial communities in batch-loaded CWs with emergent (Typha angustifolia), submerged (Hydrilla verticillata), and floating plant (Salvinia natans) were analyzed by 454 pyrosequencing technology. After six periods of experiment, the TCS removal efficiencies were over 90% in CWs, and negative effects of TCS on bacterial community richness and diversity were observed. Moreover, plant species effect existed. Bacterial strains that contributed to TCS biodegradation in CWs were successfully identified. In TCS-treated T. angustifolia and H. verticillata CWs, beta-Proteobacteria increased by 16.63% and 18.20%, respectively. In TCS-treated S. natans CWs, delta- and gamma-Proteobacteria and Sphingobacteria increased by 9.36%, 19.49%, and 31.37%, respectively, and could relate to TCS biodegradation. TCS affected the development of certain bacteria, and eventually, the bacterial community structures in CWs. This research provided ecologically relevant information on bacterial community and microbial removal mechanism in CWs under TCS treatment.
authors.affiliation: School of Environmental Science and Engineering, Shandong University, Jinan 250100, China.
authors.forename: Congcong
authors.initials: C
authors.lastname: Zhao
authors.affiliation: Environment Research Institute, Shandong University, Jinan 250100, China.
authors.forename: HuiJun
authors.initials: H
authors.lastname: Xie
authors.affiliation: School of Municipal and Environmental Engineering, Shandong Jianzhu University, Jinan 250101, China.
authors.forename: Jingtao
authors.initials: J
authors.lastname: Xu
authors.affiliation: School of Environmental Science and Engineering, Shandong University, Jinan 250100, China.
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Xu
authors.affiliation: School of Environmental Science and Engineering, Shandong University, Jinan 250100, China. Electronic address: zhangjian00@sdu.edu.cn.
authors.forename: Jian
authors.initials: J
authors.lastname: Zhang
authors.affiliation: School of Environmental Science and Engineering, Shandong University, Jinan 250100, China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Hu
authors.affiliation: Department of Mathematics and Statistics, Texas Tech University, Broadway and Boston, Lubbock, TX 79409-1042, USA.
authors.forename: Cui
authors.initials: C
authors.lastname: Liu
authors.affiliation: School of Environmental Science and Engineering, Shandong University, Jinan 250100, China.
authors.forename: Shuang
authors.initials: S
authors.lastname: Liang
authors.affiliation: School of Environmental Science and Engineering, Shandong University, Jinan 250100, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Environment Research Institute, Shandong University, Jinan 250100, China.
authors.forename: Jingmin
authors.initials: J
authors.lastname: Wang
mesh_terms: D001419:Bacteria / Q000145:classification / Q000378:metabolism*; D001673:Biodegradation, Environmental; D052918:Environmental Restoration and Remediation / Q000379:methods*; D014260:Triclosan / Q000032:analysis / Q000378:metabolism*; D014865:Waste Disposal, Fluid; D014874:Water Pollutants, Chemical / Q000032:analysis / Q000378:metabolism*; D053833:Wetlands
chemical_list: D014874:Water Pollutants, Chemical; D014260:Triclosan
keywords: Constructed wetlands; Microbial community; Microbial removal mechanism; Plant species effect; Triclosan