title: Two fundamental questions about protein evolution.
abstract: Two basic questions are considered that approach protein evolution from different directions; the problems arising from using Markov models for the deeper divergences, and then the origin of proteins themselves. The real problem for the first question (going backwards in time) is that at deeper phylogenies the Markov models of sequence evolution must lose information exponentially at deeper divergences, and several testable methods are suggested that should help resolve these deeper divergences. For the second question (coming forwards in time) a problem is that most models for the origin of protein synthesis do not give a role for the very earliest stages of the process. From our knowledge of the importance of replication accuracy in limiting the length of a coding molecule, a testable hypothesis is proposed. The length of the code, the code itself, and tRNAs would all have prior roles in increasing the accuracy of RNA replication; thus proteins would have been formed only after the tRNAs and the length of the triplet code are already formed. Both questions lead to testable predictions.
authors.affiliation: Institute of Fundamental Sciences, Massey University, Palmerston North, New Zealand. Electronic address: d.penny@massey.ac.nz.
authors.forename: David
authors.initials: D
authors.lastname: Penny
authors.affiliation: Institute of Fundamental Sciences, Massey University, Palmerston North, New Zealand.
authors.forename: Bojian
authors.initials: B
authors.lastname: Zhong
mesh_terms: D000818:Animals; D003062:Codon; D004261:DNA Replication; D019143:Evolution, Molecular; D006801:Humans; D008390:Markov Chains; D008957:Models, Genetic; D008958:Models, Molecular; D009690:Nucleic Acid Conformation; D000069497:Origin of Life; D010802:Phylogeny; D020543:Proteome / Q000235:genetics* / Q000378:metabolism; D020871:RNA Stability; D012343:RNA, Transfer / Q000737:chemistry / Q000378:metabolism
chemical_list: D003062:Codon; D020543:Proteome; D012343:RNA, Transfer
keywords: Eigen limit; Markov models; Origin of proteins; Phylogenetics