title: Interchange of L polymerase protein between two strains of viral hemorrhagic septicemia virus (VHSV) genotype IV alters temperature sensitivities in vitro.
abstract: Viral hemorrhagic septicemia virus (VHSV) has four genotypes (I-IV) and sub-lineages within genotype I and IV. Using a reverse genetics approach, we explored the importance of the L gene for growth characteristics at different temperatures following interchange of the L gene within genotype IV (IVa and IVb) strains. VHSV strains harboring heterologous L gene were recovered and we show that the L gene determines growth characteristics at different temperatures in permissive cell lines.
authors.affiliation: Norwegian University of Life Sciences, PO Box 8146 Dep, N-0033 Oslo, Norway.
authors.forename: Sung-Hyun
authors.initials: SH
authors.lastname: Kim
authors.affiliation: Institute of Marine & Environmental Technology, University of Maryland Baltimore County, Baltimore, MD 21202, USA.
authors.forename: Shamila
authors.initials: S
authors.lastname: Yusuff
authors.affiliation: Institute of Marine & Environmental Technology, University of Maryland Baltimore County, Baltimore, MD 21202, USA.
authors.forename: Vikram N
authors.initials: VN
authors.lastname: Vakharia
authors.affiliation: Norwegian University of Life Sciences, PO Box 8146 Dep, N-0033 Oslo, Norway. Electronic address: oystein.evensen@nmbu.no.
authors.forename: Øystein
authors.initials: Ø
authors.lastname: Evensen
mesh_terms: D000818:Animals; D002460:Cell Line; D005399:Fishes; D005838:Genotype; D029141:Novirhabdovirus / Q000201:enzymology* / Q000235:genetics / Q000502:physiology* / Q000528:radiation effects; D012324:RNA-Dependent RNA Polymerase / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D059386:Reverse Genetics; D013696:Temperature; D014779:Virus Replication / Q000528:radiation effects*
chemical_list: D011994:Recombinant Proteins; D012324:RNA-Dependent RNA Polymerase
keywords: Genotype IV; L protein; Reverse genetics; Temperature sensitivity; Viral hemorrhagic septicemia virus