title: Insoluble protein assemblies characterized by fourier transform infrared spectroscopy.
abstract: Fourier transform infrared (FTIR) spectroscopy is a useful tool for the structural characterization of insoluble protein assemblies, as it allows to obtain information on the protein secondary structures and on their intermolecular interactions. The protocols for FTIR spectroscopy and microspectroscopy measurements in transmission and attenuated total reflection modes will be presented and illustrated in the following examples: bacterial inclusion bodies, self-assembling peptides, thermal aggregates, and amyloid fibrils.
authors.affiliation: Department of Biotechnology and Biosciences, University of Milano-Bicocca, Piazza della Scienza 2, Milan, 20126, Italy, antonino.natalello@unimib.it.
authors.forename: Antonino
authors.initials: A
authors.lastname: Natalello
authors.forename: Silvia M
authors.initials: SM
authors.lastname: Doglia
mesh_terms: D000682:Amyloid / Q000737:chemistry*; D000818:Animals; D001419:Bacteria / Q000378:metabolism; D006801:Humans; D002479:Inclusion Bodies / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry*; D017433:Protein Structure, Secondary; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*
chemical_list: D000682:Amyloid; D010455:Peptides