title: Segmental isotope-labeling of the intrinsically disordered protein PQBP1.
abstract: Polyglutamine tract-binding protein 1 (PQBP1) is an intrinsically disordered protein abundantly expressed in the brain. Mutations in the PQBP1 gene are causative for X-linked mental retardation disorders. Here, we investigated the structure of the C-terminal segment within the context of full-length PQBP1. We produced a segmentally isotope-labeled PQBP1 composed of a non-labeled segment (residues 1-219; N-segment) and a (13)C/(15)N-labeled segment (residues 220-265; C-segment). Our results demonstrate that the segmental isotope-labeling combined with NMR spectroscopy is useful for detecting a very weak intra-molecular interaction in an intrinsically disordered protein.
authors.affiliation: Faculty of Pharmaceutical Sciences, University of Toyama, 2630 Sugitani, Toyama 930-0194, Japan; Graduate School of Innovative Life Science, University of Toyama, 2630 Sugitani, Toyama 930-0194, Japan.
authors.forename: Yuko
authors.initials: Y
authors.lastname: Nabeshima
authors.affiliation: Faculty of Pharmaceutical Sciences, University of Toyama, 2630 Sugitani, Toyama 930-0194, Japan; Graduate School of Innovative Life Science, University of Toyama, 2630 Sugitani, Toyama 930-0194, Japan. Electronic address: mineyuki@pha.u-toyama.ac.jp.
authors.forename: Mineyuki
authors.initials: M
authors.lastname: Mizuguchi
authors.affiliation: Faculty of Pharmaceutical Sciences, University of Toyama, 2630 Sugitani, Toyama 930-0194, Japan.
authors.forename: Asagi
authors.initials: A
authors.lastname: Kajiyama
authors.affiliation: Department of Neuropathology, Medical Research Institute, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8510, Japan; Center for Brain Integration Research, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8510, Japan.
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Okazawa
mesh_terms: D064267:Intrinsically Disordered Proteins / Q000737:chemistry* / Q000378:metabolism*; D007553:Isotope Labeling; D009682:Magnetic Resonance Spectroscopy; D009687:Nuclear Proteins / Q000737:chemistry* / Q000378:metabolism*
chemical_list: D064267:Intrinsically Disordered Proteins; D009687:Nuclear Proteins
keywords: Expressed protein ligation; Intra-molecular interaction; Intrinsically disordered protein; NMR; Segmental isotope-labeling