title: Blueberry anthocyanins-enriched extracts attenuate the cyclophosphamide-induced lung toxicity.
abstract: The influence of blueberry anthocyanins-enriched extracts (BAE) on cyclophosphamide (CTX)-induced lung damage was investigated. BAE (20 and 80mg/kg/d) were orally dosed to rats 7d both before and after CTX administration (100mg/kg, intraperitoneal injection, single dose). The results showed CTX treatment induced obvious pathological pulmonary injury with raised injury score and lung/body weight ratio. In CTX group, the activity of lysosomal proteases, lung permeability and the number of neutrophil infiltrates all elevated. On the other hand, claudin-4 and zonula occluden-1 protein levels decreased. And also changes of oxidative stress and inflammatory cytokines parameters together with nuclear factor-κB activation were shown. Improvement of all above-mentioned physiological and biochemical parameters was exhibited in BAE groups, with a dose-dependent manner. In conclusion, BAE attenuate the CTX-induced lung toxicity, antioxidant and anti-inflammatory characteristics are involved in the protective mechanism of BAE.
authors.affiliation: College of Food, Shenyang Agricultural University, Shenyang City 110866, China.
authors.forename: Dehong
authors.initials: D
authors.lastname: Tan
authors.affiliation: Emergency Medicine, Laboratory of Rescue Center of Severe Wound and Trauma, PLA, Shenyang 110016, China; Department of General Hospital of Shenyang Military Command, Laboratory of Rescue Center of Severe Wound and Trauma, PLA, Shenyang 110016, China.
authors.forename: Yunen
authors.initials: Y
authors.lastname: Liu
authors.affiliation: College of Food, Shenyang Agricultural University, Shenyang City 110866, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Shi
authors.affiliation: College of Food, Shenyang Agricultural University, Shenyang City 110866, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Li
authors.affiliation: College of Food, Shenyang Agricultural University, Shenyang City 110866, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Liu
authors.affiliation: College of Food, Shenyang Agricultural University, Shenyang City 110866, China.
authors.forename: Bing
authors.initials: B
authors.lastname: Bai
authors.affiliation: College of Food, Shenyang Agricultural University, Shenyang City 110866, China. Electronic address: mengxjsy@126.com.
authors.forename: Xianjun
authors.initials: X
authors.lastname: Meng
authors.affiliation: Emergency Medicine, Laboratory of Rescue Center of Severe Wound and Trauma, PLA, Shenyang 110016, China; Department of General Hospital of Shenyang Military Command, Laboratory of Rescue Center of Severe Wound and Trauma, PLA, Shenyang 110016, China. Electronic address: houmingxiao188@163.com.
authors.forename: Mingxiao
authors.initials: M
authors.lastname: Hou
authors.affiliation: College of Food, Shenyang Agricultural University, Shenyang City 110866, China.
authors.forename: Xiaoxu
authors.initials: X
authors.lastname: Liu
authors.affiliation: College of Food, Shenyang Agricultural University, Shenyang City 110866, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Sheng
authors.affiliation: College of Food, Shenyang Agricultural University, Shenyang City 110866, China.
authors.forename: Xinyue
authors.initials: X
authors.lastname: Luo
mesh_terms: D000818:Animals; D000872:Anthocyanins / Q000494:pharmacology*; D000970:Antineoplastic Agents / Q000633:toxicity; D029798:Blueberry Plants; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology; D003520:Cyclophosphamide / Q000037:antagonists & inhibitors* / Q000633:toxicity*; D016207:Cytokines / Q000096:biosynthesis; D008168:Lung / Q000187:drug effects* / Q000473:pathology / Q000503:physiopathology; D055370:Lung Injury / Q000139:chemically induced / Q000503:physiopathology / Q000517:prevention & control; D008297:Male; D009504:Neutrophils / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects; D010936:Plant Extracts / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D000872:Anthocyanins; D000970:Antineoplastic Agents; D016207:Cytokines; D010936:Plant Extracts; D003520:Cyclophosphamide
keywords: Antioxidant; Cytokine; Nuclear factor-κB; Rat; Tight junctions