title: Light-dependent segregation of begomoviruses in Asystasia gangetica leaves.
abstract: Asystasia gangetica (Acanthaceae) from tropical Africa and Asia is used as source of food and for medical applications. Plants collected in West Africa in the 1980s with typical geminivirus symptoms showed an unusual symptom segregation that included vein yellowing, curling and mosaic, which were present simultaneously or separately on different leaves of the same plant or on different plants propagated as cuttings from a single plant. Rolling-circle amplification in combination with restriction fragment length polymorphism analysis followed by deep sequencing of the RCA products identified two geminiviruses in these plants. One with a bipartite genome, Asystasia begomovirus 1, and the other with a monopartite genome together with its defective DNA, Asystasia begomovirus 2. The relationship between leaf symptoms and virus distribution under different light regimes was investigated, and showed for the first time an unusual segregation of symptoms and viruses, either within a single plant, or even within a leaf.
authors.affiliation: Biologisches Institut, Abteilung für Molekularbiologie und Virologie der Pflanzen, Universität Stuttgart, Pfaffenwaldring 57, D-70550 Stuttgart, Germany.
authors.forename: Patricia
authors.initials: P
authors.lastname: Wyant
authors.affiliation: Biologisches Institut, Abteilung für Molekularbiologie und Virologie der Pflanzen, Universität Stuttgart, Pfaffenwaldring 57, D-70550 Stuttgart, Germany.
authors.forename: Stephan
authors.initials: S
authors.lastname: Strohmeier
authors.affiliation: Biologisches Institut, Abteilung für Molekularbiologie und Virologie der Pflanzen, Universität Stuttgart, Pfaffenwaldring 57, D-70550 Stuttgart, Germany.
authors.forename: Alexander
authors.initials: A
authors.lastname: Fischer
authors.affiliation: Biologisches Institut, Abteilung für Molekularbiologie und Virologie der Pflanzen, Universität Stuttgart, Pfaffenwaldring 57, D-70550 Stuttgart, Germany.
authors.forename: Benjamin
authors.initials: B
authors.lastname: Schäfer
authors.affiliation: National Institute for Biotechnology and Genetic Engineering, Jhang Road, Faisalabad, Pakistan.
authors.forename: Rob W
authors.initials: RW
authors.lastname: Briddon
authors.affiliation: Lehrstuhl für Biochemie, Department Biologie, Staudtstr. 5, 91058 Erlangen, Germany.
authors.forename: Björn
authors.initials: B
authors.lastname: Krenz
authors.affiliation: Biologisches Institut, Abteilung für Molekularbiologie und Virologie der Pflanzen, Universität Stuttgart, Pfaffenwaldring 57, D-70550 Stuttgart, Germany. Electronic address: holger.jeske@bio.uni-stuttgart.de.
authors.forename: Holger
authors.initials: H
authors.lastname: Jeske
mesh_terms: D027901:Acanthaceae / Q000528:radiation effects* / Q000821:virology*; D053839:Begomovirus / Q000235:genetics / Q000302:isolation & purification*; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics; D008027:Light; D008969:Molecular Sequence Data; D010935:Plant Diseases / Q000821:virology*; D018515:Plant Leaves / Q000528:radiation effects / Q000821:virology; D017422:Sequence Analysis, DNA
chemical_list: D004279:DNA, Viral
keywords: Asystasia gangetica; Circomics; Geminivirus; Light dependence; RCA; Segregation of symptoms