title: Tetracycline-inducible gene expression system in Leishmania mexicana.
abstract: Here we present a T7-driven, tetracycline-inducible system for protein expression in human pathogen Leishmania mexicana. The gene expression in this strain is tightly regulated and dose- and time-dependent. This system can be widely used by the parasitology community to analyze effects of genes of interest on biology, physiology and virulence of parasites causing cutaneous leishmaniases.
authors.affiliation: Life Science Research Centre, Faculty of Science, University of Ostrava, 710 00 Ostrava, Czech Republic.
authors.forename: Natalya
authors.initials: N
authors.lastname: Kraeva
authors.affiliation: Life Science Research Centre, Faculty of Science, University of Ostrava, 710 00 Ostrava, Czech Republic.
authors.forename: Aygul
authors.initials: A
authors.lastname: Ishemgulova
authors.affiliation: Biology Centre, Institute of Parasitology, Czech Academy of Sciences, 370 05 České Budějovice (Budweis), Czech Republic; Faculty of Sciences, University of South Bohemia, 370 05 České Budějovice (Budweis), Czech Republic; Canadian Institute for Advanced Research, Toronto, ON M5G 1Z8, Canada.
authors.forename: Julius
authors.initials: J
authors.lastname: Lukeš
authors.affiliation: Life Science Research Centre, Faculty of Science, University of Ostrava, 710 00 Ostrava, Czech Republic; Biology Centre, Institute of Parasitology, Czech Academy of Sciences, 370 05 České Budějovice (Budweis), Czech Republic. Electronic address: vyacheslav.yurchenko@osu.cz.
authors.forename: Vyacheslav
authors.initials: V
authors.lastname: Yurchenko
mesh_terms: D015870:Gene Expression; D006801:Humans; D007894:Leishmania mexicana / Q000187:drug effects* / Q000235:genetics* / Q000378:metabolism; D016773:Leishmaniasis, Cutaneous / Q000469:parasitology*; D015800:Protozoan Proteins / Q000235:genetics* / Q000378:metabolism; D013752:Tetracycline / Q000494:pharmacology*
chemical_list: D015800:Protozoan Proteins; D013752:Tetracycline
keywords: Gene expression; Leishmania mexicana; Tet-inducible system