title: Identification of heat shock cognate protein 70 gene (Alhsc70) of Apolygus lucorum and its expression in response to different temperature and pesticide stresses.
abstract: Heat shock cognate protein 70 (Hsc70) is a very important stress-resistance protein of insects against environmental stresses. We employed fluorescent real-time quantitative polymerase chain reaction and Western-blot techniques to analyze the transcriptional and translational expression profiles of AlHSC70 under extreme temperature (4°C and 40°C) or 4 pesticide stresses in Apolygus lucorum. The results showed that the expression of AlHSC70 were significantly induced by cyhalothrin or extremely high temperature (40°C) in both transcriptional and translational levels (P < 0.05), while the transcriptional and translational level of AlHSC70 decreased significantly in treatments of chlorpyrifos or extreme cold temperature (4°C) (P < 0.05). Moreover, after Apolygus lucorum treated by imidacloprid or emamectin benzoate, the expression of AlHSC70 was only up-regulated significantly at the transcriptional level (P < 0.05), although obviously up-regulated at the translational level of AlHSC70. Therefore, this study confirmed that the Alhsc70 gene played important roles in response to both temperature and pesticide stresses, especially for cyhalothrin or extremely high temperature (40°C). In addition, the significant polynomial regression correlations between temperature and the Alhsc70 expression level were shown in all the nymph and adult stages (P < 0.01), indicating temperature was an important factor to affect the relative expression of Alhsc70.
authors.affiliation: Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Nanjing, China.|Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Sun
authors.affiliation: Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Nanjing, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhao
authors.affiliation: Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Nanjing, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Sheng
authors.affiliation: Entomology and Nematology, Mid-Florida Research and Education Center, University of Florida, Apopka, Florida, USA.
authors.forename: Ying-Fang
authors.initials: YF
authors.lastname: Xiao
authors.affiliation: Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China.
authors.forename: Yong-Jun
authors.initials: YJ
authors.lastname: Zhang
authors.affiliation: Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Nanjing, China.
authors.forename: Li-Xin
authors.initials: LX
authors.lastname: Bai
authors.affiliation: Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Nanjing, China.
authors.forename: Yongan
authors.initials: Y
authors.lastname: Tan
authors.affiliation: Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Nanjing, China.
authors.forename: Liu-Bin
authors.initials: LB
authors.lastname: Xiao
authors.affiliation: Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Nanjing, China.
authors.forename: Guang-Chun
authors.initials: GC
authors.lastname: Xu
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D005786:Gene Expression Regulation / Q000187:drug effects; D018840:HSP70 Heat-Shock Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D020062:Heteroptera / Q000187:drug effects / Q000235:genetics* / Q000502:physiology; D019476:Insect Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D010575:Pesticides / Q000494:pharmacology*; D010802:Phylogeny; D013312:Stress, Physiological / Q000187:drug effects* / Q000235:genetics*; D013696:Temperature; D014158:Transcription, Genetic / Q000187:drug effects
chemical_list: D018840:HSP70 Heat-Shock Proteins; D019476:Insect Proteins; D010575:Pesticides
keywords: Apolygus lucorum; expression files; extreme temperature; hsc70; pesticide