title: The toxic effects of melamine on spleen lymphocytes with or without cyanuric acid in mice.
abstract: Melamine is an organic nitrogenous compound whose acute toxicity was generally thought to be low in animals. In the present work, we investigated the potential cytotoxic effects of melamine on spleen lymphocytes in mice. In the treated group, morphological changes were observed in cultured lymphocytes in vitro. The co-administration of melamine and cyanuric acid caused a declining tendency in stimulation index of spleen lymphocyte. All treated groups had lower ratios of CD4+/CD8+. Both early apoptotic and late apoptotic/necrotic rates of lymphocyte were significantly higher in the co-administration high groups of melamine and cyanuric acid. Melamine-related toxicity promoted the expression of Bax mRNA, and suppressed the expression of Bcl-2 mRNA in spleen of the treated mice. These results provided useful information for assessing the toxicity of melamine on immune system of mammals, and contributed to the existing toxic profile of melamine.
authors.affiliation: College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang 110866, China.
authors.forename: Rong H
authors.initials: RH
authors.lastname: Yin
authors.affiliation: College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang 110866, China; Shenyang Entry-Exit Inspection and Quarantine Bureau, Shenyang 110016, China.
authors.forename: Jiao
authors.initials: J
authors.lastname: Liu
authors.affiliation: College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang 110866, China.
authors.forename: Hua S
authors.initials: HS
authors.lastname: Li
authors.affiliation: College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang 110866, China. Electronic address: wlb7411@163.com.
authors.forename: Wen L
authors.initials: WL
authors.lastname: Bai
authors.affiliation: Research Academy of Animal Husbandry and Veterinary Medicine Sciences of Jilin Province, Changchun 130062, China.
authors.forename: Rong L
authors.initials: RL
authors.lastname: Yin
authors.affiliation: College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang 110866, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Wang
authors.affiliation: College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang 110866, China.
authors.forename: Wen C
authors.initials: WC
authors.lastname: Wang
authors.affiliation: College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang 110866, China.
authors.forename: Bao S
authors.initials: BS
authors.lastname: Liu
authors.affiliation: College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang 110866, China.
authors.forename: Xiao H
authors.initials: XH
authors.lastname: Han
authors.affiliation: College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang 110866, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Han
authors.affiliation: College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang 110866, China.
authors.forename: Jian B
authors.initials: JB
authors.lastname: He
authors.affiliation: College of Land and Environment, Shenyang Agricultural University, Shenyang 110866, China.
authors.forename: Xiao R
authors.initials: XR
authors.lastname: Han
mesh_terms: D000818:Animals; D004785:Environmental Pollutants / Q000633:toxicity*; D005786:Gene Expression Regulation / Q000187:drug effects; D008214:Lymphocytes / Q000187:drug effects*; D008297:Male; D051379:Mice; D012117:Resins, Synthetic / Q000633:toxicity*; D013154:Spleen / Q000187:drug effects* / Q000503:physiopathology; D014227:Triazines / Q000633:toxicity*
chemical_list: D004785:Environmental Pollutants; D012117:Resins, Synthetic; D014227:Triazines; C004632:cyanuric acid; C011907:melamine
keywords: Cyanuric acid; Melamine; Mouse; Spleen lymphocytes; Toxicity