title: Molecular characterization of peste-des-petits ruminants virus (PPRV) isolated from an outbreak in the Indo-Bangladesh border of Tripura state of North-East India.
abstract: Peste-des-petits- ruminants (PPR) is a highly contagious and devastating disease of goats and sheep. Although India is endemic for PPR, Tripura, a state in North East India has never been reported confirmed PPR outbreaks. Recently, an outbreak of PPR occurred in non-descript goats at the Sabroom town of Tripura state in North-East India in June, 2013. The causative agent, PPR virus (PPRV) was confirmed by sandwich ELISA, virus isolation and N gene based RT-PCR and sequencing. The sequence and phylogenetic analysis confirmed the involvement of lineage IV PPR virus in the outbreak. The outbreak viruses from Tripura state were clustered mainly with circulating viruses from Bangladesh, India, China, Pakistan, Tajikistan, Dubai and Kurdistan. However, the nucleotide sequence homology ranged from 99.2 to 99.6% with the PPR strains circulating in Bangladesh during 2011 and 2012 whereas 95.5-98% homology has been observed with the viruses from India and other countries. These findings suggest the transboundary circulation of PPR virus between India and Bangladesh border, which warrant immediate vaccination across the international border to create an immune belt.
authors.affiliation: Division of Virology, Indian Veterinary Research Institute, Mukteswar Campus, Nainital 263 138, Uttarakhand, India. Electronic address: drchelva@gmail.com.
authors.forename: Dhanavelu
authors.initials: D
authors.lastname: Muthuchelvan
authors.affiliation: Department of Veterinary Physiology and Biochemistry, College of Veterinary Sciences & Animal Husbandry, R.K. Nagar, West Tripura 799008, Tripura, India.
authors.forename: Ankan
authors.initials: A
authors.lastname: De
authors.affiliation: Department of Veterinary Physiology and Biochemistry, College of Veterinary Sciences & Animal Husbandry, R.K. Nagar, West Tripura 799008, Tripura, India.
authors.forename: Bikas
authors.initials: B
authors.lastname: Debnath
authors.affiliation: Division of Virology, Indian Veterinary Research Institute, Mukteswar Campus, Nainital 263 138, Uttarakhand, India.
authors.forename: Dheeraj
authors.initials: D
authors.lastname: Choudhary
authors.affiliation: Division of Virology, Indian Veterinary Research Institute, Mukteswar Campus, Nainital 263 138, Uttarakhand, India.
authors.forename: Gnanavel
authors.initials: G
authors.lastname: Venkatesan
authors.affiliation: Division of Virology, Indian Veterinary Research Institute, Mukteswar Campus, Nainital 263 138, Uttarakhand, India.
authors.forename: Kaushal Kishore
authors.initials: KK
authors.lastname: Rajak
authors.affiliation: Division of Virology, Indian Veterinary Research Institute, Mukteswar Campus, Nainital 263 138, Uttarakhand, India.
authors.forename: Shashi Bhusan
authors.initials: SB
authors.lastname: Sudhakar
authors.affiliation: Project Directorate on Animal Disease Monitoring and Surveillance, Hebbal, Bangalore 560024, Karnataka, India.
authors.forename: Divakar
authors.initials: D
authors.lastname: Himadri
authors.affiliation: Division of Virology, Indian Veterinary Research Institute, Mukteswar Campus, Nainital 263 138, Uttarakhand, India.
authors.forename: Awadh Bihari
authors.initials: AB
authors.lastname: Pandey
authors.affiliation: National Institute of Animal Biotechnology, Hyderabad, AP, India; The Pirbright Institute, Ash Road, Pirbright, Surrey GU24 0NF, UK.
authors.forename: Satya
authors.initials: S
authors.lastname: Parida
mesh_terms: D000818:Animals; D001459:Bangladesh / Q000453:epidemiology; D001483:Base Sequence; D004196:Disease Outbreaks / Q000662:veterinary*; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D006041:Goats; D007194:India / Q000453:epidemiology; D008969:Molecular Sequence Data; D029021:Peste-des-Petits-Ruminants / Q000453:epidemiology* / Q000821:virology; D018107:Peste-des-petits-ruminants virus / Q000235:genetics* / Q000302:isolation & purification; D010802:Phylogeny; D017422:Sequence Analysis, DNA / Q000662:veterinary; D014611:Vaccination / Q000662:veterinary*
keywords: Molecular epidemiology; North-East India; PPR antigen detection; Peste-des-petits -ruminants virus; Transboundary movement; Tripura