title: A modular peptide-based immobilization system for ZrO2, TiZr and TiO2 surfaces.
abstract: The present study describes a novel versatile immobilization system for the modification of implant materials with biologically active molecules (BAMs), e.g. antibiotics or growth factors. Specific adsorbing peptides are used as anchor molecules to immobilize oligodesoxynucleotides (ODNs) on the implant surface (anchor strand, AS). The BAM is conjugated to a complementary ODN strand (CS) which is able to hybridize to the AS on the implant surface to immobilize the BAM. The ODN double strand allows for a controlled release of the BAM adjustable by the ODN sequence and length. The immobilization system was developed and proven on three typical implant materials, namely ZrO2, TiZr and Ti, respectively. The parathyroid hormone (PTH) fragment 1-34 was conjugated to the CS and immobilized on these different implant materials. To investigate the biological activity of the immobilized PTH, alkaline phosphatase was quantified after incubation of the osteoblast precursor cells C2C12 on the modified samples. The results demonstrate the successful immobilization of biologically active PTH (1-34) and the high potential of the established surfaces to achieve an increased osseointegration of variable implants, especially for patients with risk factors.
authors.affiliation: Chair of Biochemistry, Technische Universität Dresden, Bergstr. 66, 01069 Dresden, Germany.
authors.forename: Tina
authors.initials: T
authors.lastname: Micksch
authors.affiliation: Chair of Biochemistry, Technische Universität Dresden, Bergstr. 66, 01069 Dresden, Germany.
authors.forename: Elisa
authors.initials: E
authors.lastname: Herrmann
authors.affiliation: Max Bergmann Center of Biomaterials, Technische Universität Dresden, Budapester Str. 27, 01069 Dresden, Germany. Electronic address: Dieter.Scharnweber@tu-dresden.de.
authors.forename: Dieter
authors.initials: D
authors.lastname: Scharnweber
authors.affiliation: Chair of Biochemistry, Technische Universität Dresden, Bergstr. 66, 01069 Dresden, Germany.
authors.forename: Bernd
authors.initials: B
authors.lastname: Schwenzer
mesh_terms: D000327:Adsorption; D000469:Alkaline Phosphatase / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D002851:Chromatography, High Pressure Liquid; D017931:DNA Primers; D004587:Electrophoresis, Agar Gel; D051379:Mice; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry*; D020349:Surface Plasmon Resonance; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry*; D015040:Zirconium / Q000737:chemistry*
chemical_list: D017931:DNA Primers; D010455:Peptides; C009495:titanium dioxide; D015040:Zirconium; D014025:Titanium; D000469:Alkaline Phosphatase; C028541:zirconium oxide
keywords: Adsorption; Oligonucleotide; Osseointegration; Peptide; Titanium