title: Characterization and identification of three novel aldo-keto reductases from Lodderomyces elongisporus for reducing ethyl 4-chloroacetoacetate.
abstract: Lodderomyces elongisporus LH703 isolated from soil samples contained three novel aldo-keto reductases (AKRs) (LEAKR 48, LEAKR 49, and LEAKR 50). The three enzymes were cloned, expressed, and purified to homogeneity for characterization. These three AKRs shared <40% amino acid identity with each other. LEAKR 50 was identified as a member of AKR3 family, whereas the other two LEAKRs were identified as members of two novel AKR families, respectively. All the three AKRs required nicotinamide adenine dinucleotide phosphate as a cofactor. However, they showed diverse characteristics, including optimum catalyzing conditions, resistance to adverse reaction conditions, and substrate specificity. LEAKR 50 was estimated to be a promising biocatalyst that could reduce ethyl 4-chloroacetoacetate with high enantiomeric excess (98% e. e.) and high activity residue under adverse conditions.
authors.affiliation: State Key Laboratory of Bioreactor Engineering, New World Institute of Biotechnology, East China University of Science and Technology, Shanghai 200237, PR China.
authors.forename: Chenxi
authors.initials: C
authors.lastname: Ning
authors.affiliation: Enzyme and Fermentation Technology Laboratory, College of Light Industry Science and Engineering, Nanjing Forestry University, Nanjing 210037, PR China. Electronic address: ezhsu@njfu.edu.cn.
authors.forename: Erzheng
authors.initials: E
authors.lastname: Su
authors.affiliation: State Key Laboratory of Bioreactor Engineering, New World Institute of Biotechnology, East China University of Science and Technology, Shanghai 200237, PR China. Electronic address: dzhwei@ecust.edu.cn.
authors.forename: Dongzhi
authors.initials: D
authors.lastname: Wei
mesh_terms: D000090:Acetoacetates / Q000737:chemistry*; D000449:Aldehyde Reductase / Q000737:chemistry* / Q000235:genetics; D000074408:Aldo-Keto Reductases; D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D005656:Fungal Proteins / Q000737:chemistry* / Q000235:genetics; D008969:Molecular Sequence Data; D009243:NAD / Q000737:chemistry*; D012442:Saccharomycopsis / Q000201:enzymology* / Q000235:genetics
chemical_list: D000090:Acetoacetates; D005656:Fungal Proteins; D009243:NAD; C111982:ethyl 4-chloro-3-oxobutanoate; D000074408:Aldo-Keto Reductases; D000449:Aldehyde Reductase
keywords: Aldo–keto reductase; Characterization; Ethyl 4-chloroacetoacetate; Identification; Lodderomyces elongisporus