title: Establishment and characterization of a bovine rectal myxoma cell line.
abstract: A new bovine cell line was developed from tumor biopsy material of rectum obtained from clinical case of 7 years old cattle with tumor mass obliterating the rectal opening. Histopathology of tumor revealed scattered stellate cells arranged singly or in clusters in loose mucinous ground substance, simulating myxoma. The cells obtained from tumor mass have been cultured for more than 36 months in DMEM supplemented with 10% fetal bovine serum (FBS). The population doubling time of this cell line was about 20.64 h. The cytogenetic analysis revealed several chromosomal abnormalities with bizarre karyotype. The origin of the cell line was confirmed by PCR amplification of 1086 bp fragment of 16s rRNA using bovine species specific primers. The new cell line would act as in vitro model to study many aspect of cancer biology such as tumor development, differentiation and therapeutics regimen to combat cancer.
authors.affiliation: Molecular Biology Lab, Division of Veterinary Biotechnology, Indian Veterinary Research Institute, Izatnagar, Bareilly 243122, India.
authors.forename: Aditya P
authors.initials: AP
authors.lastname: Sahoo
authors.affiliation: Molecular Biology Lab, Division of Veterinary Biotechnology, Indian Veterinary Research Institute, Izatnagar, Bareilly 243122, India. Electronic address: aktiwari63@yahoo.com.
authors.forename: Ashok K
authors.initials: AK
authors.lastname: Tiwari
authors.affiliation: Molecular Biology Lab, Division of Veterinary Biotechnology, Indian Veterinary Research Institute, Izatnagar, Bareilly 243122, India.
authors.forename: G
authors.initials: G
authors.lastname: Ravi Kumar
authors.affiliation: Molecular Biology Lab, Division of Veterinary Biotechnology, Indian Veterinary Research Institute, Izatnagar, Bareilly 243122, India.
authors.forename: U
authors.initials: U
authors.lastname: Chaturvedi
authors.affiliation: Molecular Biology Lab, Division of Veterinary Biotechnology, Indian Veterinary Research Institute, Izatnagar, Bareilly 243122, India.
authors.forename: Lakshya
authors.initials: L
authors.lastname: Veer Singh
authors.affiliation: Molecular Biology Lab, Division of Veterinary Biotechnology, Indian Veterinary Research Institute, Izatnagar, Bareilly 243122, India.
authors.forename: Shikha
authors.initials: S
authors.lastname: Saxena
authors.affiliation: Molecular Biology Lab, Division of Veterinary Biotechnology, Indian Veterinary Research Institute, Izatnagar, Bareilly 243122, India.
authors.forename: S K
authors.initials: SK
authors.lastname: Palia
authors.affiliation: Govind Ballabh Pant University of Agriculture and Technology, Pantnagar 263145, India.
authors.forename: N S
authors.initials: NS
authors.lastname: Jadon
authors.affiliation: Molecular Biology Lab, Division of Veterinary Biotechnology, Indian Veterinary Research Institute, Izatnagar, Bareilly 243122, India.
authors.forename: R
authors.initials: R
authors.lastname: Singh
authors.affiliation: Molecular Biology Lab, Division of Veterinary Biotechnology, Indian Veterinary Research Institute, Izatnagar, Bareilly 243122, India.
authors.forename: K P
authors.initials: KP
authors.lastname: Singh
authors.affiliation: NBAGR, Karnal 132001, India.
authors.forename: B S
authors.initials: BS
authors.lastname: Brahmaprakash
authors.affiliation: Molecular Biology Lab, Division of Veterinary Biotechnology, Indian Veterinary Research Institute, Izatnagar, Bareilly 243122, India.
authors.forename: S K
authors.initials: SK
authors.lastname: Maiti
authors.affiliation: Govind Ballabh Pant University of Agriculture and Technology, Pantnagar 263145, India.
authors.forename: A K
authors.initials: AK
authors.lastname: Das
mesh_terms: D000818:Animals; D002417:Cattle; D045744:Cell Line, Tumor; D006801:Humans; D066298:In Vitro Techniques; D009232:Myxoma / Q000473:pathology*; D012004:Rectal Neoplasms / Q000473:pathology*
keywords: Biopsy material; Bovine cell line; Chromosomal abnormalities; Myxoma; Rectum; Species specific primers