title: Extreme furfural tolerance of a soil bacterium Enterobacter cloacae GGT036.
abstract: Detoxification process of cellular inhibitors including furfural is essential for production of bio-based chemicals from lignocellulosic biomass. Here we isolated an extreme furfural-tolerant bacterium Enterobacter cloacae GGT036 from soil sample collected in Mt. Gwanak, Republic of Korea. Among isolated bacteria, only E. cloacae GGT036 showed cell growth with 35 mM furfural under aerobic culture. Compared to the maximal half inhibitory concentration (IC50) of well-known industrial strains Escherichia coli (24.9 mM furfural) and Corynebacterium glutamicum (10 mM furfural) based on the cell density, IC50 of E. cloacae GGT036 (47.7 mM) was significantly higher after 24 h, compared to E. coli and C. glutamicum. Since bacterial cell growth was exponentially inhibited depending on linearly increased furfural concentrations in the medium, we concluded that E. cloacae GGT036 is an extreme furfural-tolerant bacterium. Recently, the complete genome sequence of E. cloacae GGT036 was announced and this could provide an insight for engineering of E. cloacae GGT036 itself or other industrially relevant bacteria.
authors.affiliation: Clean Energy Research Center, Korea Institute of Science and Technology, Hwarang-ro 14-gil 5, Seongbuk-gu, Seoul 136-791, Republic of Korea; Green School (Graduate School of Energy and Environment), 145 Anam-ro, Seongbuk-gu, Seoul, Republic of Korea.
authors.forename: Sun Young
authors.initials: SY
authors.lastname: Choi
authors.affiliation: Clean Energy Research Center, Korea Institute of Science and Technology, Hwarang-ro 14-gil 5, Seongbuk-gu, Seoul 136-791, Republic of Korea.
authors.forename: Gyeongtaek
authors.initials: G
authors.lastname: Gong
authors.affiliation: Clean Energy Research Center, Korea Institute of Science and Technology, Hwarang-ro 14-gil 5, Seongbuk-gu, Seoul 136-791, Republic of Korea; Green School (Graduate School of Energy and Environment), 145 Anam-ro, Seongbuk-gu, Seoul, Republic of Korea.
authors.forename: Hong-Sil
authors.initials: HS
authors.lastname: Park
authors.affiliation: Clean Energy Research Center, Korea Institute of Science and Technology, Hwarang-ro 14-gil 5, Seongbuk-gu, Seoul 136-791, Republic of Korea; Department of Clean Energy and Chemical Engineering, Korea University of Science and Technology, 217 Gajeong-ro, Yuseong-gu, Daejeon 305-350, Republic of Korea.
authors.forename: Youngsoon
authors.initials: Y
authors.lastname: Um
authors.affiliation: Green School (Graduate School of Energy and Environment), 145 Anam-ro, Seongbuk-gu, Seoul, Republic of Korea; Department of Chemical and Biological Engineering, Korea University, 145 Anam-ro, Seongbuk-gu, Seoul, Republic of Korea.
authors.forename: Sang Jun
authors.initials: SJ
authors.lastname: Sim
authors.affiliation: Clean Energy Research Center, Korea Institute of Science and Technology, Hwarang-ro 14-gil 5, Seongbuk-gu, Seoul 136-791, Republic of Korea; Green School (Graduate School of Energy and Environment), 145 Anam-ro, Seongbuk-gu, Seoul, Republic of Korea; Department of Clean Energy and Chemical Engineering, Korea University of Science and Technology, 217 Gajeong-ro, Yuseong-gu, Daejeon 305-350, Republic of Korea. Electronic address: hmwoo@kist.re.kr.
authors.forename: Han Min
authors.initials: HM
authors.lastname: Woo
mesh_terms: D016972:Enterobacter cloacae / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology*; D005662:Furaldehyde / Q000378:metabolism / Q000633:toxicity*; D005663:Furans / Q000378:metabolism / Q000633:toxicity; D020128:Inhibitory Concentration 50; D050296:Microbial Viability; D012987:Soil; D012988:Soil Microbiology
chemical_list: D005663:Furans; D012987:Soil; C012986:furfuryl alcohol; D005662:Furaldehyde
keywords: Detoxification; Enterobacter cloacae; Furfural tolerance; IC(50)