title: Effect of percutaneous catheter drainage on pancreatic injury in rats with severe acute pancreatitis induced by sodium taurocholate.
abstract: OBJECTIVE
  This study investigated the effect of percutaneous catheter drainage (PCD) on pancreatic injury in severe acute pancreatitis (SAP) rats.
  
  
  METHODS
  Sixty Wistar rats were equally randomized into three groups: a sham operated control group, an SAP control group, and a PCD group. The levels of inflammatory cytokines, the activity of group II phospholipase A2 (PLA2) in blood and ascitic fluid, and the pancreas level of group II PLA2 and trypsin activity were measured 24 h after the operation. The apoptosis of the pancreatic cells, the expression of cycloxygenase-2 (COX-2), inducible nitric oxide synthase (iNOS), active caspase-3, Bcl-2 and Bax in the pancreas was detected. Pancreatic pathological changes were observed.
  
  
  RESULTS
  The levels of proinflammatory cytokines, the activity of group II PLA2 and trypsin activity in pancreas in the SAP group were higher than those in the PCD group. The histopathological results revealed that the pancreatic injury was alleviated in the PCD group. The expression of COX-2 and iNOS in the pancreatic tissue in the SAP control rats was higher than that in the PCD rats. The expression of Bcl-2 was decreased and the expression of active caspase-3 and Bax was increased in the pancreas of PCD rats. The apoptosis index of the pancreatic cells in the PCD rats was higher than that in the SAP control rats.
  
  
  CONCLUSIONS
  PCD can relieve SAP-induced pancreatic injury by inhibiting inflammatory reactions, and promoting apoptosis of pancreatic cells.
authors.affiliation: General Surgery Center, Chengdu Military General Hospital, No. 270, Rongdu Road, Jingniu District, Chengdu, China.
authors.forename: Guang-yu
authors.initials: GY
authors.lastname: Chen
authors.affiliation: General Surgery Center, Chengdu Military General Hospital, No. 270, Rongdu Road, Jingniu District, Chengdu, China.
authors.forename: Rui-wu
authors.initials: RW
authors.lastname: Dai
authors.affiliation: General Surgery Center, Chengdu Military General Hospital, No. 270, Rongdu Road, Jingniu District, Chengdu, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Luo
authors.affiliation: General Surgery Center, Chengdu Military General Hospital, No. 270, Rongdu Road, Jingniu District, Chengdu, China.
authors.forename: Wei-hui
authors.initials: WH
authors.lastname: Liu
authors.affiliation: General Surgery Center, Chengdu Military General Hospital, No. 270, Rongdu Road, Jingniu District, Chengdu, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Chen
authors.affiliation: General Surgery Center, Chengdu Military General Hospital, No. 270, Rongdu Road, Jingniu District, Chengdu, China.
authors.forename: Ning
authors.initials: N
authors.lastname: Lin
authors.affiliation: General Surgery Center, Chengdu Military General Hospital, No. 270, Rongdu Road, Jingniu District, Chengdu, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Wang
authors.affiliation: General Surgery Center, Chengdu Military General Hospital, No. 270, Rongdu Road, Jingniu District, Chengdu, China.
authors.forename: Guo-de
authors.initials: GD
authors.lastname: Luo
authors.affiliation: General Surgery Center, Chengdu Military General Hospital, No. 270, Rongdu Road, Jingniu District, Chengdu, China. Electronic address: acecgy@gmail.com.
authors.forename: Li-jun
authors.initials: LJ
authors.lastname: Tang
mesh_terms: D000208:Acute Disease; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D015153:Blotting, Western; D002756:Cholagogues and Choleretics; D016207:Cytokines / Q000378:metabolism; D004322:Drainage / Q000379:methods*; D007150:Immunohistochemistry; D008297:Male; D010179:Pancreas / Q000378:metabolism / Q000473:pathology*; D010195:Pancreatitis / Q000139:chemically induced / Q000378:metabolism / Q000473:pathology / Q000628:therapy*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D013656:Taurocholic Acid; D016896:Treatment Outcome
chemical_list: D015415:Biomarkers; D002756:Cholagogues and Choleretics; D016207:Cytokines; D013656:Taurocholic Acid
keywords: Apoptosis; Ascitic fluid collection; Inflammation; Pancreatic injury; Percutaneous catheter drainage; Severe acute pancreatitis