title: Lipid antigen presentation through CD1d pathway in mouse lung epithelial cells, macrophages and dendritic cells and its suppression by poly-dispersed single-walled carbon nanotubes.
abstract: Effect of poly-dispersed acid-functionalized single-walled carbon nanotubes (AF-SWCNTs) was examined on lipid antigen presentation through CD1d pathway on three cell lines, LA4, MHS, and JAWSII used as prototype antigen presenting cells (APCs). CD1d molecule was expressed on 80-90% MHS (prototype macrophages) and JAWSII (prototype dendritic cells) cells whereas <5% LA4 cells (lung epithelial cells, non-classical APCs) expressed CD1d. Treatment with AF-SWCNTs but not with pristine SWCNTs resulted in a significant decline in the level of CD1d mRNA as well as mRNA levels of some other intracellular proteins involved in lipid antigen presentation pathway (MTP, ApoE, prosaposin, SR-BI and LDLr). Lipid antigen presentation was assessed by first incubating the cells with a prototype lipid antigen (α-Glactosylceramide or αGC) and then staining with L363 monoclonal antibody that detects αGC bound to CD1d molecule. While 100% MHS and JAWSII cells presented αGC, only 20% LA4 cells presented the CD1d antigen. Treatment with AF-SWCNTs resulted in a 30-40% decrease in αGC antigen presentation in all three cell lines. These results show that AF-SWCNT treatment down regulated the lipid antigen presentation pathway in all three cell lines and significantly lowered the ability of these cell lines to present αGC antigen.
authors.affiliation: School of Life Sciences, Jawaharlal Nehru University, New Delhi, India.
authors.forename: Zaigham Abbas
authors.initials: ZA
authors.lastname: Rizvi
authors.affiliation: School of Life Sciences, Jawaharlal Nehru University, New Delhi, India.
authors.forename: Niti
authors.initials: N
authors.lastname: Puri
authors.affiliation: Faculty of Life Sciences and Biotechnology, South Asian University, New Delhi, India. Electronic address: rajivksaxena@hotmail.com.
authors.forename: Rajiv K
authors.initials: RK
authors.lastname: Saxena
mesh_terms: D000818:Animals; D017951:Antigen Presentation / Q000187:drug effects*; D055610:Antigens, CD1d / Q000276:immunology*; D002460:Cell Line; D045744:Cell Line, Tumor; D003713:Dendritic Cells / Q000187:drug effects* / Q000276:immunology; D004847:Epithelial Cells / Q000187:drug effects* / Q000276:immunology; D005699:Galactosylceramides / Q000276:immunology; D008264:Macrophages / Q000187:drug effects* / Q000276:immunology; D051379:Mice; D037742:Nanotubes, Carbon
chemical_list: D055610:Antigens, CD1d; C584692:CD1d antigen, mouse; D005699:Galactosylceramides; D037742:Nanotubes, Carbon
keywords: CD1d; Carbon nanotubes; L363 mab; Lipid antigen presentation; Lungs; α-Glactosylceramide