title: Studies on cytotoxic pregnane sapogenins from Cynanchum wilfordii.
abstract: To investigate their cytotoxicity, seventeen C21-steroidal pregnane sapogenins 1-17 were isolated from the hydrolytic extracts of the roots of Cynanchum wilfordii. Among them, sapogenins 1-7 are new compounds, whose structures were determined by extensive analysis of spectroscopic data and X-ray crystallographic analysis. Especially, sapogenins with salicyl or vanilloyl group, and a new aberrant pregnane skeleton with ether linkage between C-12 and C-20 were found for the first time. Compound 1 revealed significant cytotoxicities on HL-60 (IC50 6.72μM) and MCF-7 (IC50 2.89μM), and compounds 14 and 15 also revealed strong inhibitory activities against K-562 (IC50 6.72μM) or MCF-7 (IC50 2.49μM), respectively.
authors.affiliation: Center for Research and Development of Fine Chemicals, Guizhou University, Guiyang 550025, PR China; The Key Laboratory of Chemistry for Natural Products of Guizhou Province and Chinese Academy of Sciences, Guiyang 550002, PR China.
authors.forename: Lie-Jun
authors.initials: LJ
authors.lastname: Huang
authors.affiliation: The Key Laboratory of Chemistry for Natural Products of Guizhou Province and Chinese Academy of Sciences, Guiyang 550002, PR China; Pharmacy Department of Tengzhou Central People Hospital, Tengzhou 277500, Shangdong Province, PR China.
authors.forename: Bing
authors.initials: B
authors.lastname: Wang
authors.affiliation: The Key Laboratory of Chemistry for Natural Products of Guizhou Province and Chinese Academy of Sciences, Guiyang 550002, PR China.
authors.forename: Jian-Xin
authors.initials: JX
authors.lastname: Zhang
authors.affiliation: The Key Laboratory of Chemistry for Natural Products of Guizhou Province and Chinese Academy of Sciences, Guiyang 550002, PR China.
authors.forename: Chen
authors.initials: C
authors.lastname: Yan
authors.affiliation: The Key Laboratory of Chemistry for Natural Products of Guizhou Province and Chinese Academy of Sciences, Guiyang 550002, PR China.
authors.forename: Shu-Zhen
authors.initials: SZ
authors.lastname: Mu
authors.affiliation: The Key Laboratory of Chemistry for Natural Products of Guizhou Province and Chinese Academy of Sciences, Guiyang 550002, PR China. Electronic address: haoxj@mail.kib.ac.cn.
authors.forename: Xiao-Jiang
authors.initials: XJ
authors.lastname: Hao
mesh_terms: D031105:Cynanchum / Q000737:chemistry*; D018922:HL-60 Cells; D006801:Humans; D020014:K562 Cells; D061986:MCF-7 Cells; D015394:Molecular Structure; D018517:Plant Roots / Q000737:chemistry; D011278:Pregnanes / Q000302:isolation & purification / Q000494:pharmacology*; D012502:Sapogenins / Q000302:isolation & purification / Q000494:pharmacology*
chemical_list: D011278:Pregnanes; D012502:Sapogenins
keywords: Antitumor; Asclepiadaceae; C(21)-steroidal sapogenins; Cynanchum wilfordii; Cytotoxicity