title: GDF15 is a novel biomarker to evaluate efficacy of pyruvate therapy for mitochondrial diseases.
abstract: Pyruvate therapy is a promising approach for the treatment of mitochondrial diseases. To identify novel biomarkers for diagnosis and to evaluate therapeutic efficacy, we performed microarray analysis of 2SD cybrid cells harboring a MELAS-causing mutation and control cells treated with either lactate or pyruvate. We found that expression and secretion of growth differentiation factor 15 (GDF15) were increased in 2SD cells treated with lactate and that serum GDF15 levels were significantly higher in patients with mitochondrial diseases than in those with other diseases, suggesting that GDF15 could be a useful marker for diagnosis and evaluating the therapeutic efficacy of pyruvate.
authors.affiliation: Research Team for Mechanism of Aging, Tokyo Metropolitan Institute of Gerontology, 35-2 Sakae-cho, Itabashi, Tokyo 173-0015, Japan.
authors.forename: Yasunori
authors.initials: Y
authors.lastname: Fujita
authors.affiliation: Research Team for Mechanism of Aging, Tokyo Metropolitan Institute of Gerontology, 35-2 Sakae-cho, Itabashi, Tokyo 173-0015, Japan.
authors.forename: Masafumi
authors.initials: M
authors.lastname: Ito
authors.affiliation: Health Support Center, Toyohashi University of Technology, 1-1 Hibarigaoka Tenpaku-cho, Toyohashi, Aichi 441-8580, Japan.
authors.forename: Toshio
authors.initials: T
authors.lastname: Kojima
authors.affiliation: Department of Pediatrics and Child Health, Kurume University School of Medicine, 67 Asahi-machi, Kurume, Fukuoka 830-0011, Japan.
authors.forename: Shuichi
authors.initials: S
authors.lastname: Yatsuga
authors.affiliation: Department of Pediatrics and Child Health, Kurume University School of Medicine, 67 Asahi-machi, Kurume, Fukuoka 830-0011, Japan.
authors.forename: Yasutoshi
authors.initials: Y
authors.lastname: Koga
authors.affiliation: Department of Genomics for Longevity and Health, Tokyo Metropolitan Institute of Gerontology, 35-2 Sakae-cho, Itabashi, Tokyo 173-0015, Japan. Electronic address: mtanaka@tmig.or.jp.
authors.forename: Masashi
authors.initials: M
authors.lastname: Tanaka
mesh_terms: D015415:Biomarkers / Q000032:analysis; D016903:Drug Monitoring / Q000379:methods*; D020869:Gene Expression Profiling; D055436:Growth Differentiation Factor 15 / Q000032:analysis*; D006801:Humans; D046228:Microarray Analysis; D028361:Mitochondrial Diseases / Q000175:diagnosis* / Q000188:drug therapy*; D019289:Pyruvic Acid / Q000378:metabolism* / Q000627:therapeutic use
chemical_list: D015415:Biomarkers; C526140:GDF15 protein, human; D055436:Growth Differentiation Factor 15; D019289:Pyruvic Acid
keywords: Biomarker; Cybrid; GDF15; Microarray; Mitochondrial diseases; Pyruvate