title: A "turn-on" fluorescent receptor for detecting tyrosine phosphopeptide using the surface imprinting procedure and the epitope approach.
abstract: A new strategy for the manufacture of a turn-on fluorescent molecularly imprinted polymer (CdTe/SiO2/MIP) receptor for detecting tyrosine phosphopeptide (pTyr peptide) was proposed. The receptor was prepared by the surface imprinting procedure and the epitope approach with silica-capped CdTe quantum dots (QDs) as core substrate and fluorescent signal, phenylphosphonic acid (PPA) as the dummy template, 1-[3-(trimethoxysilyl) propyl] urea as the functional monomer, and octyltrimethoxysilane as the cross-linker. The synthetic CdTe/SiO2/MIP was able to selectively capture the template PPA and corresponding target pTyr peptide with fluorescence enhancement via the special interaction between them and the recognition cavities. The receptor exhibited the linear fluorescence enhancement to pTyr peptide in the range of 0.5-35μM, and the detection limit was 0.37μM. The precision for five replicate detections of pTyr peptide at 20μM was 2.60% (relative standard deviation). Combining the fluorescence property of the CdTe QDs with the merits of the surface imprinting technique and the epitope approach, the receptor not only owned high recognition site accessibility and good binding affinities for target pTyr peptide, but also improved the fluorescence selectivity of the CdTe QDs, as well revealed the feasibility of fabrication of a turn-on fluorescence probe using the surface imprinting procedure and the epitope approach.
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology (Nankai University), Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), and Research Center for Analytical Sciences, College of Chemistry, Nankai University, Tianjin 300071, PR China.
authors.forename: Dong-Yan
authors.initials: DY
authors.lastname: Li
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology (Nankai University), Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), and Research Center for Analytical Sciences, College of Chemistry, Nankai University, Tianjin 300071, PR China.
authors.forename: Ya-Ping
authors.initials: YP
authors.lastname: Qin
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology (Nankai University), Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), and Research Center for Analytical Sciences, College of Chemistry, Nankai University, Tianjin 300071, PR China.
authors.forename: Hong-Yu
authors.initials: HY
authors.lastname: Li
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology (Nankai University), Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), and Research Center for Analytical Sciences, College of Chemistry, Nankai University, Tianjin 300071, PR China.
authors.forename: Xi-Wen
authors.initials: XW
authors.lastname: He
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology (Nankai University), Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), and Research Center for Analytical Sciences, College of Chemistry, Nankai University, Tianjin 300071, PR China. Electronic address: wyli@nankai.edu.cn.
authors.forename: Wen-You
authors.initials: WY
authors.lastname: Li
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology (Nankai University), Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), and Research Center for Analytical Sciences, College of Chemistry, Nankai University, Tianjin 300071, PR China; National Chromatographic Research and Analysis Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116011, PR China.
authors.forename: Yu-Kui
authors.initials: YK
authors.lastname: Zhang
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D002104:Cadmium / Q000737:chemistry; D000939:Epitopes / Q000737:chemistry; D005455:Fluorescent Antibody Technique; D005456:Fluorescent Dyes / Q000737:chemistry; D054802:Molecular Imprinting; D010748:Phosphopeptides / Q000302:isolation & purification*; D045663:Quantum Dots / Q000737:chemistry; D012822:Silicon Dioxide / Q000737:chemistry; D013499:Surface Properties; D013691:Tellurium / Q000737:chemistry; D014443:Tyrosine / Q000302:isolation & purification*
chemical_list: D000939:Epitopes; D005456:Fluorescent Dyes; D010748:Phosphopeptides; D002104:Cadmium; D014443:Tyrosine; D012822:Silicon Dioxide; D013691:Tellurium
keywords: Epitope approach; Fluorescence enhancement; Quantum dots; Surface imprinting; Tyrosine phosphopeptide