title: Transient expression in HEK 293 cells: an alternative to E. coli for the production of secreted and intracellular mammalian proteins.
abstract: Transient transfection of human embryonic kidney cells (HEK 293) enables the rapid and affordable lab-scale production of recombinant proteins. In this chapter protocols for the expression and purification of both secreted and intracellular proteins using transient expression in HEK 293 cells are described.
authors.affiliation: OPPF-UK, Research Complex at Harwell, R92 Rutherford Appleton Laboratories, Harwell Oxford, Didcot, OX11 0FA, UK, joanne@strubi.ox.ac.uk.
authors.forename: Joanne E
authors.initials: JE
authors.lastname: Nettleship
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Watson
authors.forename: Nahid
authors.initials: N
authors.lastname: Rahman-Huq
authors.forename: Louise
authors.initials: L
authors.lastname: Fairall
authors.forename: Mareike G
authors.initials: MG
authors.lastname: Posner
authors.forename: Abhishek
authors.initials: A
authors.lastname: Upadhyay
authors.forename: Yamini
authors.initials: Y
authors.lastname: Reddivari
authors.forename: Jonathan M G
authors.initials: JM
authors.lastname: Chamberlain
authors.forename: Simon E
authors.initials: SE
authors.lastname: Kolstoe
authors.forename: Stefan
authors.initials: S
authors.lastname: Bagby
authors.forename: John W R
authors.initials: JW
authors.lastname: Schwabe
authors.forename: Raymond J
authors.initials: RJ
authors.lastname: Owens
mesh_terms: D000818:Animals; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D057809:HEK293 Cells / Q000378:metabolism*; D006801:Humans; D008322:Mammals / Q000235:genetics* / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics* / Q000378:metabolism; D014162:Transfection / Q000379:methods
chemical_list: D011994:Recombinant Proteins