title: Improving the odorant sensitivity of olfactory receptor-expressing yeast with accessory proteins.
abstract: Olfaction depends on the selectivity and sensitivity of olfactory receptors. Previous attempts at constructing a mammalian olfactory receptor-based artificial odorant sensing system in the budding yeast Saccharomyces cerevisiae suffered from low sensitivity and activity. This result may be at least in part due to poor functional expression of olfactory receptors and/or limited solubility of some odorants in the medium. In this study, we examined the effects of two types of accessory proteins, receptor transporting protein 1 short and odorant binding proteins, in improving odor-mediated activation of olfactory receptors expressed in yeast. We found that receptor transporting protein 1 short enhanced the membrane expression and ligand-induced responses of some olfactory receptors. Coexpression of odorant binding proteins of the silkworm moth Bombyx mori enhanced the sensitivity of a mouse olfactory receptor. Our results suggest that different classes of accessory proteins can confer sensitive and robust responses of olfactory receptors expressed in yeast. Inclusion of accessory proteins may be essential in the future development of practical olfactory receptor-based odorant sensors.
authors.affiliation: Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology, Koganei, Tokyo 184-8588, Japan.
authors.forename: Yosuke
authors.initials: Y
authors.lastname: Fukutani
authors.affiliation: Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology, Koganei, Tokyo 184-8588, Japan.
authors.forename: Ayaka
authors.initials: A
authors.lastname: Hori
authors.affiliation: Graduate School of Bio-Applications and Systems Engineering, Tokyo University of Agriculture and Technology, Koganei, Tokyo 184-8588, Japan.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Tsukada
authors.affiliation: Graduate School of Bio-Applications and Systems Engineering, Tokyo University of Agriculture and Technology, Koganei, Tokyo 184-8588, Japan.
authors.forename: Ryoichi
authors.initials: R
authors.lastname: Sato
authors.affiliation: Organization of Advanced Science and Technology, Kobe University, Nada, Kobe 657-8501, Japan.
authors.forename: Jun
authors.initials: J
authors.lastname: Ishii
authors.affiliation: Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, Nada, Kobe 657-8501, Japan.
authors.forename: Akihiko
authors.initials: A
authors.lastname: Kondo
authors.affiliation: Department of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, NC 27710, USA.
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Matsunami
authors.affiliation: Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology, Koganei, Tokyo 184-8588, Japan. Electronic address: yohda@cc.tuat.ac.jp.
authors.forename: Masafumi
authors.initials: M
authors.lastname: Yohda
mesh_terms: D000818:Animals; D012831:Bombyx / Q000235:genetics; D002462:Cell Membrane / Q000378:metabolism; D015870:Gene Expression; D005818:Genetic Engineering; D019476:Insect Proteins / Q000235:genetics; D026901:Membrane Transport Proteins / Q000235:genetics / Q000378:metabolism; D051379:Mice; D009812:Odorants; D021381:Protein Transport; D018035:Receptors, Odorant / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000187:drug effects / Q000235:genetics*
chemical_list: D019476:Insect Proteins; D026901:Membrane Transport Proteins; D018035:Receptors, Odorant; C045648:odorant-binding protein
keywords: Biosensing; Odorant binding protein; Olfactory receptor; Receptor transport protein; Saccharomyces cerevisiae