title: Chemical composition and acaricidal activity of the essential oil of Baccharis dracunculifolia De Candole (1836) and its constituents nerolidol and limonene on larvae and engorged females of Rhipicephalus microplus (Acari: Ixodidae).
abstract: Baccharis dracunculifolia DC (common name "alecrim-do-campo" in Brazil) is a plant with widespread distribution in South America that is the botanical origin of green propolis. The aim of this study was to evaluate the chemical composition and acaricidal activity of the essential oil of B. dracunculifolia and its constituents nerolidol and limonene on unengorged larvae and engorged females of Rhipicephalus microplus (Acari: Ixodidae). The essential oil yield was 0.8% of dry mass and the major constituents were nerolidol (22.3%), germacrene D (7.2%), limonene (6.9%), β-pinene (6.7) and bicyclogermacrene (6.5%). The acaricidal activity of the essential oil and the pure compounds nerolidol and (R)-(+)-limonene were assessed in the laboratory through the modified larval packet test (LPT) and the female immersion test (FIT). In the LPT, the essential oil and nerolidol were both active, causing more than 90% mortality at concentrations from 15.0 and 10.0 mg mL(-1), respectively, whereas (R)-(+)-limonene was not active. In the FIT, the oil and nerolidol caused reduction in the quantity and quality of eggs produced, with control percentages of 96.3% and 90.3% at concentrations of 60.0 and 50.0 mg mL(-1), respectively. It can be concluded that the essential oil obtained from the aerial parts of B. dracunculifolia and its major component nerolidol have high activity on R. microplus larvae and engorged females.
authors.affiliation: Grupo de Química Supramolecular e Biomimética (GQSB), Departamento de Química, Universidade Federal de Viçosa (UFV), Campus Universitário, Avenida P.H. Rolfs, s/n, Viçosa, MG, 36570-900, Brazil.
authors.forename: Tiago Coelho
authors.initials: TC
authors.lastname: de Assis Lage
authors.affiliation: Grupo de Química Supramolecular e Biomimética (GQSB), Departamento de Química, Universidade Federal de Viçosa (UFV), Campus Universitário, Avenida P.H. Rolfs, s/n, Viçosa, MG, 36570-900, Brazil.
authors.forename: Ricardo Marques
authors.initials: RM
authors.lastname: Montanari
authors.affiliation: Grupo de Química Supramolecular e Biomimética (GQSB), Departamento de Química, Universidade Federal de Viçosa (UFV), Campus Universitário, Avenida P.H. Rolfs, s/n, Viçosa, MG, 36570-900, Brazil. Electronic address: santonio@ufv.br.
authors.forename: Sergio Antonio
authors.initials: SA
authors.lastname: Fernandes
authors.affiliation: Programa de Pós-graduação em Ciências Biológicas, Comportamento e Biologia Animal, Instituto de Ciências Biológicas, Departamento de Zoologia da Universidade Federal de Juiz de Fora, 36036-900 Juiz de Fora, MG, Brazil.
authors.forename: Caio Márcio
authors.initials: CM
authors.lastname: de Oliveira Monteiro
authors.affiliation: Programa de Pós-graduação em Ciências Veterinárias, Instituto de Ciências Veterinárias, Departamento de Parasitologia, Universidade Federal Rural do Rio de Janeiro, 23890-000 Seropédica, RJ, Brazil.
authors.forename: Tatiane
authors.initials: T
authors.lastname: de Oliveira Souza Senra
authors.affiliation: Programa de Pós-graduação em Ciências Veterinárias, Instituto de Ciências Veterinárias, Departamento de Parasitologia, Universidade Federal Rural do Rio de Janeiro, 23890-000 Seropédica, RJ, Brazil.
authors.forename: Viviane
authors.initials: V
authors.lastname: Zeringota
authors.affiliation: Programa de Pós-graduação em Ciências Biológicas, Comportamento e Biologia Animal, Instituto de Ciências Biológicas, Departamento de Zoologia da Universidade Federal de Juiz de Fora, 36036-900 Juiz de Fora, MG, Brazil.
authors.forename: Renata
authors.initials: R
authors.lastname: da Silva Matos
authors.affiliation: Programa de Pós-graduação em Ciências Biológicas, Comportamento e Biologia Animal, Instituto de Ciências Biológicas, Departamento de Zoologia da Universidade Federal de Juiz de Fora, 36036-900 Juiz de Fora, MG, Brazil.
authors.forename: Erik
authors.initials: E
authors.lastname: Daemon
mesh_terms: D056810:Acaricides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D000818:Animals; D031175:Baccharis / Q000737:chemistry*; D053138:Cyclohexenes / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D005260:Female; D007814:Larva / Q000187:drug effects; D000077222:Limonene; D009822:Oils, Volatile / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D010938:Plant Oils / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D048494:Rhipicephalus / Q000187:drug effects*; D012717:Sesquiterpenes / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D013729:Terpenes / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology
chemical_list: D056810:Acaricides; D053138:Cyclohexenes; D009822:Oils, Volatile; D010938:Plant Oils; D012717:Sesquiterpenes; D013729:Terpenes; D000077222:Limonene; C037055:nerolidol
keywords: Alecrim-do-campo; Cattle ticks; Limonene; Nerolidol; Terpenes