title: Separation of nucleobases, nucleosides, and nucleotides using two zwitterionic silica-based monolithic capillary columns coupled with tandem mass spectrometry.
abstract: The capability of employing synthesized zwitterionic silica-based monolithic capillary columns (140 mm × 0.1mm) for separation of highly polar and hydrophilic nucleobases, nucleosides, and nucleotides in hydrophilic interaction chromatography is reported. The suitability of the columns for on-line conjunction with electrospray tandem mass spectrometry was explored. Our results show that the grafted layer of zwitterionic monomer ([2-(methacryloyloxy)ethyl]-dimethyl-(3-sulfopropyl)-ammonium hydroxide or 2-methacryloyloxyethyl phosphorylcholine) on the silica monolithic surface significantly improved the separation selectivity and reproducibility, as compared to the bare silica monolith. The stepwise elution from 90% to 70% of acetonitrile enabled separation of a complex sample mixture containing 21 compounds with a total analysis time less than 40 min.
authors.affiliation: Institute of Analytical Chemistry of the ASCR, v. v. i., Veveří 97, 60200 Brno, Czech Republic. Electronic address: moravcova@iach.cz.
authors.forename: Dana
authors.initials: D
authors.lastname: Moravcová
authors.affiliation: Faculty of Pharmacy, P.O. Box 56, 00014 University of Helsinki, Finland.
authors.forename: Markus
authors.initials: M
authors.lastname: Haapala
authors.affiliation: Institute of Analytical Chemistry of the ASCR, v. v. i., Veveří 97, 60200 Brno, Czech Republic.
authors.forename: Josef
authors.initials: J
authors.lastname: Planeta
authors.affiliation: Steno Diabetes Center, Niels Steensens Vej 2, 2820 Gentofte, Denmark.
authors.forename: Tuulia
authors.initials: T
authors.lastname: Hyötyläinen
authors.affiliation: Faculty of Pharmacy, P.O. Box 56, 00014 University of Helsinki, Finland.
authors.forename: Risto
authors.initials: R
authors.lastname: Kostiainen
authors.affiliation: Department of Chemistry, P.O. Box 55, 00014 University of Helsinki, Finland. Electronic address: susanne.wiedmer@helsinki.fi.
authors.forename: Susanne K
authors.initials: SK
authors.lastname: Wiedmer
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods; D057927:Hydrophobic and Hydrophilic Interactions; D009705:Nucleosides / Q000737:chemistry / Q000302:isolation & purification*; D009711:Nucleotides / Q000737:chemistry / Q000302:isolation & purification*; D015203:Reproducibility of Results; D012822:Silicon Dioxide / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization / Q000295:instrumentation / Q000379:methods*; D013499:Surface Properties; D053719:Tandem Mass Spectrometry / Q000295:instrumentation / Q000379:methods*
chemical_list: D009705:Nucleosides; D009711:Nucleotides; D012822:Silicon Dioxide
keywords: ESI–MS; HILIC separation; Monolithic silica column; Nucleobase; Nucleoside; Nucleotide