title: Simultaneous determination of uric Acid and xanthine using a poly(methylene blue) and electrochemically reduced graphene oxide composite film modified electrode.
abstract: Poly(methylene blue) and electrochemically reduced graphene oxide composite film modified electrode (PMB-ERGO/GCE) was successfully fabricated by electropolymerization and was used for simultaneous determination of uric acid (UA) and xanthine (Xa). Based on the excellent electrocatalytic activity of PMB-ERGO/GCE, the electrochemical behaviors of UA and Xa were studied by cyclic voltammetry (CV) and square wave voltammetry (SWV). Two anodic sensitive peaks at 0.630 V (versus Ag/AgCl) for UA and 1.006 V (versus Ag/AgCl) for Xa were given by CV in pH 3.0 phosphate buffer. The calibration curves for UA and Xa were obtained in the range of 8.00 × 10(-8)~4.00 × 10(-4) M and 1.00 × 10(-7)~4.00 × 10(-4) M, respectively, by SWV. The detection limits for UA and Xa were 3.00 × 10(-8) M and 5.00 × 10(-8) M, respectively. Finally, the proposed method was applied to simultaneously determine UA and Xa in human urine with good selectivity and high sensitivity.
authors.affiliation: Department of Chemistry and Materials Science, Huaibei Normal University, Huaibei 235000, China.
authors.forename: Gen
authors.initials: G
authors.lastname: Liu
authors.affiliation: Department of Chemistry and Materials Science, Huaibei Normal University, Huaibei 235000, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Ma
authors.affiliation: Department of Chemistry and Materials Science, Huaibei Normal University, Huaibei 235000, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Luo
authors.affiliation: Department of Chemistry and Materials Science, Huaibei Normal University, Huaibei 235000, China.
authors.forename: Deng-Ming
authors.initials: DM
authors.lastname: Sun
authors.affiliation: Department of Chemistry and Materials Science, Huaibei Normal University, Huaibei 235000, China.
authors.forename: Shuang
authors.initials: S
authors.lastname: Shao