title: Ellagic acid suppresses lipid accumulation by suppressing early adipogenic events and cell cycle arrest.
abstract: Ellagic acid (EA) is a natural polyphenol found in various fruits and vegetables. In this study, we examined the inhibitory effect of EA on fat accumulation in 3T3-L1 cells during adipogenesis. Our data showed that EA reduced fat accumulation by down-regulating adipogenic markers such as peroxisome proliferator activated receptor γ (PPARγ) and the CCAAT/enhancer binding protein α (C/EBPα) at the mRNA and protein levels in a dose-dependent manner. We found that the decrease in adipogenic markers resulted from reduced expression of some early adipogenic transcription factors such as KLF4, KLF5, Krox20, and C/EBPβ within 24 h. Also, these inhibitions were correlated with down-regulation of TG synthetic enzymes, causing inhibition of triglyceride (TG) levels in 3T3-L1 cells investigated by ORO staining and in zebrafish investigated by TG assay. Additionally, the cell cycle analysis showed that EA inhibited cell cycle progression by arresting cells at the G0/G1 phase.
authors.affiliation: Department of Biomedical Science, CHA University, Kyonggi, 463-836, South Korea.
authors.forename: Mi-Seon
authors.initials: MS
authors.lastname: Woo
authors.forename: Hyeon-Son
authors.initials: HS
authors.lastname: Choi
authors.forename: Min-Jung
authors.initials: MJ
authors.lastname: Seo
authors.forename: Hui-Jeon
authors.initials: HJ
authors.lastname: Jeon
authors.forename: Boo-Yong
authors.initials: BY
authors.lastname: Lee
mesh_terms: D041721:3T3-L1 Cells; D017667:Adipocytes / Q000187:drug effects*; D050156:Adipogenesis / Q000187:drug effects*; D000818:Animals; D022763:CCAAT-Enhancer-Binding Protein-alpha / Q000378:metabolism; D022782:CCAAT-Enhancer-Binding Protein-beta / Q000235:genetics; D059447:Cell Cycle Checkpoints; D015536:Down-Regulation; D004610:Ellagic Acid / Q000494:pharmacology*; D000090062:Kruppel-Like Factor 4; D050356:Lipid Metabolism / Q000187:drug effects*; D051379:Mice; D047495:PPAR gamma / Q000378:metabolism; D059808:Polyphenols / Q000494:pharmacology; D014157:Transcription Factors / Q000378:metabolism; D015027:Zebrafish
chemical_list: D022763:CCAAT-Enhancer-Binding Protein-alpha; D022782:CCAAT-Enhancer-Binding Protein-beta; C000715468:Klf4 protein, mouse; D000090062:Kruppel-Like Factor 4; D047495:PPAR gamma; D059808:Polyphenols; D014157:Transcription Factors; D004610:Ellagic Acid
keywords: 3T3-L1 celles; adipogenesis; adipogenic markers; ellagic acid; zebrafish