title: Influence of environmental variables on the structure and composition of soil bacterial communities in natural and constructed wetlands.
abstract: Bacteria are key players in wetland ecosystems, however many essential aspects regarding the ecology of wetland bacterial communities remain unknown. The present study characterizes soil bacterial communities from natural and constructed wetlands through the pyrosequencing of 16S rDNA genes in order to evaluate the influence of wetland variables on bacterial community composition and structure. The results show that the composition of soil bacterial communities was significantly associated with the wetland type (natural or constructed wetland), the type of environment (lagoon, Typha or Salix) and three continuous parameters (SOM, COD and TKN). However, no clear associations were observed with soil pH. Bacterial diversity values were significantly lower in the constructed wetland with the highest inlet nutrient concentrations. The abundances of particular metabolic groups were also related to wetland characteristics.
authors.affiliation: Instituto de Medio Ambiente, Recursos Naturales y Biodiversidad, Universidad de León, Calle La Serna, no. 56, CP 24071, León, Spain. Electronic address: parrh@unileon.es.
authors.forename: Paula
authors.initials: P
authors.lastname: Arroyo
authors.affiliation: Departamento de Biología Molecular, Universidad de León, Campus de Vegazana s/n, CP 24071, León, Spain. Electronic address: luis.saenzdemiera@unileon.es.
authors.forename: Luis E
authors.initials: LE
authors.lastname: Sáenz de Miera
authors.affiliation: Departamento de Biodiversidad y Gestión Ambiental, Universidad de León, Campus de Vegazana s/n, CP 24071, León, Spain. Electronic address: gemma.ansola@unileon.es.
authors.forename: Gemma
authors.initials: G
authors.lastname: Ansola
mesh_terms: D001419:Bacteria / Q000145:classification* / Q000254:growth & development; D017753:Ecosystem; D052918:Environmental Restoration and Remediation / Q000379:methods; D012987:Soil / Q000737:chemistry*; D012988:Soil Microbiology; D014865:Waste Disposal, Fluid / Q000379:methods; D053833:Wetlands
chemical_list: D012987:Soil
keywords: 16S rRNA pyrosequencing; Bacterial community composition; Diversity; Soil and water physicochemistry; Soil wetland bacterial community