title: Effect of actual long-term spaceflight on BDNF, TrkB, p75, BAX and BCL-XL genes expression in mouse brain regions.
abstract: Mice of C57BL/6J strain were exposed to 1-month spaceflight on Russian biosatellite Bion-M1 to determine the effect of long-term actual spaceflight on the expression of genes involved in the processes of neurogenesis and apoptosis. Specifically, we focused on the genes encoding proapoptotic factor BAX, antiapoptotic factor BCL-XL, brain-derived neurotrophic factor (BDNF) and BDNF receptors TrkB and p75. Spaceflight reduced the expression of the antiapoptotic BCL-XL gene in the striatum and hypothalamus, but increased it in the hippocampus. To estimate environmental stress contribution into spaceflight effects we analyzed spaceflight-responsive genes in mice housed for 1 month on Earth in the same shuttle cabins that were used for spaceflight, and in mice of the laboratory control group. It was shown that 1-month shuttle cabin housing decreased BCL-XL gene expression in the striatum but failed to alter BCL-XL mRNA levels in the hippocampus or hypothalamus. Spaceflight failed to alter the expression of the proapoptotic BAX gene in all investigated brain structures, although the insignificant increase of the BAX mRNA level in the hippocampus of spaceflight mice was found. At the same time, shuttle cabin housing produced insignificant decrease in BAX gene expression in the hippocampus. In contrast to the BCL-XL gene, genes encoding BAX, BDNF as well as TrkB and p75 receptors did not respond to 30-day spaceflight. Thus, long-term spaceflight (1) did not affect the expression of genes encoding BDNF as well as TrkB and p75 receptors, (2) produced dysregulation in genetic control of the neuronal apoptosis, (3) implicated BCL-XL as the risk factor for spaceflight-induced behavioral abnormalities.
authors.affiliation: Institute of Cytology and Genetics, Lavrentyeva Avenue, 10, 633090 Novosibirsk, Russia. Electronic address: naumenko2002@bionet.nsc.ru.
authors.forename: V S
authors.initials: VS
authors.lastname: Naumenko
authors.affiliation: Institute of Cytology and Genetics, Lavrentyeva Avenue, 10, 633090 Novosibirsk, Russia.
authors.forename: A V
authors.initials: AV
authors.lastname: Kulikov
authors.affiliation: Institute of Cytology and Genetics, Lavrentyeva Avenue, 10, 633090 Novosibirsk, Russia.
authors.forename: E M
authors.initials: EM
authors.lastname: Kondaurova
authors.affiliation: Institute of Cytology and Genetics, Lavrentyeva Avenue, 10, 633090 Novosibirsk, Russia.
authors.forename: A S
authors.initials: AS
authors.lastname: Tsybko
authors.affiliation: Institute of Cytology and Genetics, Lavrentyeva Avenue, 10, 633090 Novosibirsk, Russia.
authors.forename: E A
authors.initials: EA
authors.lastname: Kulikova
authors.affiliation: Institute of Biomedical Problems, Khoroshevskoe Street, 76a, 123007 Moscow, Russia.
authors.forename: I B
authors.initials: IB
authors.lastname: Krasnov
authors.affiliation: Institute of Biomedical Problems, Khoroshevskoe Street, 76a, 123007 Moscow, Russia.
authors.forename: B S
authors.initials: BS
authors.lastname: Shenkman
authors.affiliation: Institute of Biomedical Problems, Khoroshevskoe Street, 76a, 123007 Moscow, Russia.
authors.forename: V N
authors.initials: VN
authors.lastname: Sychev
authors.affiliation: Institute of Cytology and Genetics, Lavrentyeva Avenue, 10, 633090 Novosibirsk, Russia.
authors.forename: E Y
authors.initials: EY
authors.lastname: Bazhenova
authors.affiliation: Institute of Cytology and Genetics, Lavrentyeva Avenue, 10, 633090 Novosibirsk, Russia.
authors.forename: N A
authors.initials: NA
authors.lastname: Sinyakova
authors.affiliation: Institute of Cytology and Genetics, Lavrentyeva Avenue, 10, 633090 Novosibirsk, Russia.
authors.forename: N K
authors.initials: NK
authors.lastname: Popova
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism*; D019208:Brain-Derived Neurotrophic Factor / Q000378:metabolism; D015870:Gene Expression; D006799:Housing, Animal; D008297:Male; D008810:Mice, Inbred C57BL; D012333:RNA, Messenger / Q000378:metabolism; D020813:Receptor, trkB / Q000378:metabolism; D017475:Receptors, Nerve Growth Factor / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013026:Space Flight; D013312:Stress, Physiological / Q000502:physiology; D051028:bcl-2-Associated X Protein / Q000378:metabolism; D051020:bcl-X Protein / Q000378:metabolism
chemical_list: C494827:Bax protein, mouse; C494812:Bcl2l1 protein, mouse; D019208:Brain-Derived Neurotrophic Factor; D012333:RNA, Messenger; D017475:Receptors, Nerve Growth Factor; C517634:Ngfr protein, mouse; D051028:bcl-2-Associated X Protein; D051020:bcl-X Protein; D020813:Receptor, trkB
keywords: BAX, BCL-X(L), BDNF, TrkB, p75 genes expression; environmental stress; mice; microgravity; spaceflight