title: Study on immobilization of yeast alcohol dehydrogenase on nanocrystalline Ni-Co ferrites as magnetic support.
abstract: The covalent binding of yeast alcohol dehydrogenase (YADH) enzyme complex in a series of magnetic crystalline Ni-Co nanoferrites, synthesized via sol-gel auto combustion technique was investigated. The structural analysis, morphology and magnetic properties of Ni-Co nanoferrites were determined by X-ray diffraction (XRD), scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDS), vibrating-sample magnetometer (VSM), high resolution transmission electron microscopy (HRTEM) and Fourier transform infrared spectroscopy (FTIR). The comparative analysis of the HRTEM micrographs of bare magnetic nanoferrite particles and particles immobilized with enzyme revealed an uniform distribution of the particles in both the cases without undergoing change in the size which was found to be in the range 20-30 nm. The binding of YADH to Ni-Co nanoferrites and the possible binding mechanism have been suggested by comparing the FTIR results. The binding properties of the immobilized YADH enzyme were also studied by kinetic parameters, optimum operational pH, temperature, thermal stability and reusability. The immobilized YADH exhibits enhanced thermal stability as compared to the free enzyme over a wide range of temperature and pH, and showed good durability after recovery by magnetic separation for repeated use.
authors.affiliation: Department of Chemistry, Aligarh Muslim University, Aligarh 202002, India. Electronic address: shakir078@yahoo.com.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Shakir
authors.affiliation: Department of Chemistry, Aligarh Muslim University, Aligarh 202002, India.
authors.forename: Zeba
authors.initials: Z
authors.lastname: Nasir
authors.affiliation: Department of Chemistry, Aligarh Muslim University, Aligarh 202002, India.
authors.forename: Mohd Shoeb
authors.initials: MS
authors.lastname: Khan
authors.affiliation: Department of Chemistry, Aligarh Muslim University, Aligarh 202002, India.
authors.lastname: Lutfullah
authors.affiliation: Interdisciplinary Biotechnology unit, Aligarh Muslim University, Aligarh 202002, UP, India.
authors.forename: Md Fazle
authors.initials: MF
authors.lastname: Alam
authors.affiliation: Interdisciplinary Biotechnology unit, Aligarh Muslim University, Aligarh 202002, UP, India.
authors.forename: Hina
authors.initials: H
authors.lastname: Younus
authors.affiliation: Department of Chemistry, College of Science, King Saud University, Riyadh, Saudi Arabia.
authors.forename: Saud Ibrahim
authors.initials: SI
authors.lastname: Al-Resayes
mesh_terms: D000426:Alcohol Dehydrogenase; D000577:Amides / Q000378:metabolism; D003035:Cobalt / Q000737:chemistry*; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000378:metabolism*; D005290:Ferric Compounds / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D060328:Magnetic Phenomena; D060306:Magnetometry; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D009532:Nickel / Q000737:chemistry*; D059027:Recycling; D012441:Saccharomyces cerevisiae / Q000201:enzymology*; D013052:Spectrometry, X-Ray Emission; D017550:Spectroscopy, Fourier Transform Infrared; D013499:Surface Properties; D013696:Temperature; D014961:X-Ray Diffraction
chemical_list: D000577:Amides; D004800:Enzymes, Immobilized; D005290:Ferric Compounds; C001215:ferrite; D003035:Cobalt; D009532:Nickel; D000426:Alcohol Dehydrogenase
keywords: Alcohol dehydrogenase; Auto-combustion; Immobilization; Nanoferrites