title: Recombinant rabies virus expressing the H protein of canine distemper virus protects dogs from the lethal distemper challenge.
abstract: The rabies virus (RV) vector LBNSE expressing foreign antigens have shown considerable promise as vaccines against viral and bacteria diseases, which is effective and safe. We produced a new RV-based vaccine vehicle expressing 1.824 kb hemagglutinin (H) gene of the canine distemper virus (CDV) by reverse genetics technology. The recombinant virus LBNSE-CDV-H retained growth properties similar to those of vector LBNSE both in BSR and mNA cell culture. The H gene of CDV was expressed and detected by immunostaining. To compare the immunogenicity of LBNSE-CDV-H, dogs were immunized with each of these recombinant viruses by intramuscular (i.m.). The dogs were bled at third weeks after the immunization for the measurement of virus neutralizing antibody (VNA) and then challenged with virulent virus (ZJ 7) at fourth weeks. The parent virus (LBNSE) without expression of any foreign molecules was included for comparison. Dogs inoculated with LBNSE-CDV-H showed no any signs of disease and exhibited seroconversion against both RV and CDV H protein. The LBNSE-CDV-H did not cause disease in dogs and conferred protection from challenge with a lethal wild type CDV strain, demonstrating its potential value for wildlife conservation efforts. Together, these studies suggest that recombinant RV expressing H protein from CDV stimulated high levels of adaptive immune responses (VNA), and protected all dogs challenge infection.
authors.affiliation: State Key Laboratory for Molecular Biology of Special Economic Animals, Institute of Special Economic Animals and Plants, Chinese Academy of Agricultural Sciences CAAS, Changchun 130112, Jilin, China.
authors.forename: Feng-Xue
authors.initials: FX
authors.lastname: Wang
authors.affiliation: State Key Laboratory for Molecular Biology of Special Economic Animals, Institute of Special Economic Animals and Plants, Chinese Academy of Agricultural Sciences CAAS, Changchun 130112, Jilin, China.
authors.forename: Shu-Qin
authors.initials: SQ
authors.lastname: Zhang
authors.affiliation: State Key Laboratory for Molecular Biology of Special Economic Animals, Institute of Special Economic Animals and Plants, Chinese Academy of Agricultural Sciences CAAS, Changchun 130112, Jilin, China.
authors.forename: Hong-Wei
authors.initials: HW
authors.lastname: Zhu
authors.affiliation: State Key Laboratory for Molecular Biology of Special Economic Animals, Institute of Special Economic Animals and Plants, Chinese Academy of Agricultural Sciences CAAS, Changchun 130112, Jilin, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Yang
authors.affiliation: State Key Laboratory for Molecular Biology of Special Economic Animals, Institute of Special Economic Animals and Plants, Chinese Academy of Agricultural Sciences CAAS, Changchun 130112, Jilin, China.
authors.forename: Na
authors.initials: N
authors.lastname: Sun
authors.affiliation: State Key Laboratory for Molecular Biology of Special Economic Animals, Institute of Special Economic Animals and Plants, Chinese Academy of Agricultural Sciences CAAS, Changchun 130112, Jilin, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Tan
authors.affiliation: State Key Laboratory for Molecular Biology of Special Economic Animals, Institute of Special Economic Animals and Plants, Chinese Academy of Agricultural Sciences CAAS, Changchun 130112, Jilin, China; Department of Pathology, University of Georgia, Athens, GA 30602, USA.
authors.forename: Zhen-Guang
authors.initials: ZG
authors.lastname: Li
authors.affiliation: State Key Laboratory for Molecular Biology of Special Economic Animals, Institute of Special Economic Animals and Plants, Chinese Academy of Agricultural Sciences CAAS, Changchun 130112, Jilin, China.
authors.forename: Shi-Peng
authors.initials: SP
authors.lastname: Cheng
authors.affiliation: Department of Pathology, University of Georgia, Athens, GA 30602, USA. Electronic address: zhenfu@uga.edu.
authors.forename: Zhen F
authors.initials: ZF
authors.lastname: Fu
authors.affiliation: State Key Laboratory for Molecular Biology of Special Economic Animals, Institute of Special Economic Animals and Plants, Chinese Academy of Agricultural Sciences CAAS, Changchun 130112, Jilin, China. Electronic address: yongjunwen@126.com.
authors.forename: Yong-Jun
authors.initials: YJ
authors.lastname: Wen
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D004216:Distemper / Q000276:immunology / Q000517:prevention & control* / Q000821:virology; D004217:Distemper Virus, Canine / Q000235:genetics / Q000276:immunology* / Q000502:physiology; D004283:Dog Diseases / Q000276:immunology / Q000517:prevention & control* / Q000821:virology; D004285:Dogs; D005260:Female; D007273:Injections, Intramuscular; D011818:Rabies / Q000276:immunology / Q000517:prevention & control* / Q000821:virology; D011820:Rabies virus / Q000235:genetics*; D014611:Vaccination; D014765:Viral Vaccines / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D014765:Viral Vaccines
keywords: Canine distemper virus; Dog; Hemagglutinin gene; Rabies virus; Replicating viral vector