title: Gene discovery in the finger leather coral Sinularia notanda by construction and sequencing of a normalized cDNA library.
abstract: The transplantation of coral fragments is one of methods that restore coral communities. To form coral colonies, the fragmented corals initiated skeletal extension from the cut-edge of fragment then success the settlement. In order to understand the molecular events underlying fragment adhesion and settlement, we constructed a normalized cDNA library and generated and annotated expressed sequence tags (ESTs) from the fragmented adult polyps of soft coral Sinularia notanda. We generated 3251 high-quality ESTs with an average length of 580 bp and the EST cluster and assembly analyses produced 2796 unigenes, including 2487 singletons and 309 contigs. Of the known genes, 55 genes were sel ected to be involved in polyp fragment adhesion and settlement based on Gene Ontology (GO) classification. Notably, two EST clones were identified to show homology with galaxin gene which was demonstrated as coral specific calcifying protein of organic matrix. These EST sequences can provide utility as molecular markers in molecular and genetic studies of S. notanda and other soft coral.
authors.affiliation: Future Aquaculture Research Center, National Fisheries Research & Development Institute, 1928, Oedo2-dong, Jeju-si, Jeju-do 690-192, Republic of Korea.
authors.forename: Jae-Woo
authors.initials: JW
authors.lastname: Kim
authors.affiliation: Future Aquaculture Research Center, National Fisheries Research & Development Institute, 1928, Oedo2-dong, Jeju-si, Jeju-do 690-192, Republic of Korea.
authors.forename: Seong Ho
authors.initials: SH
authors.lastname: Kim
authors.affiliation: Future Aquaculture Research Center, National Fisheries Research & Development Institute, 1928, Oedo2-dong, Jeju-si, Jeju-do 690-192, Republic of Korea.
authors.forename: Min-Min
authors.initials: MM
authors.lastname: Jung
authors.affiliation: Future Aquaculture Research Center, National Fisheries Research & Development Institute, 1928, Oedo2-dong, Jeju-si, Jeju-do 690-192, Republic of Korea.
authors.forename: Heung Soo
authors.initials: HS
authors.lastname: Kim
authors.affiliation: Future Aquaculture Research Center, National Fisheries Research & Development Institute, 1928, Oedo2-dong, Jeju-si, Jeju-do 690-192, Republic of Korea.
authors.forename: Seock-Jung
authors.initials: SJ
authors.lastname: Han
authors.affiliation: Future Aquaculture Research Center, National Fisheries Research & Development Institute, 1928, Oedo2-dong, Jeju-si, Jeju-do 690-192, Republic of Korea.
authors.forename: Tae Seok
authors.initials: TS
authors.lastname: Moon
authors.affiliation: Future Aquaculture Research Center, National Fisheries Research & Development Institute, 1928, Oedo2-dong, Jeju-si, Jeju-do 690-192, Republic of Korea.
authors.forename: Bong-Seok
authors.initials: BS
authors.lastname: Kim
authors.affiliation: Biotechnology Research Division, National Fisheries Research & Development Institute, 152-1, Haean-ro, Gijang-eup, Gijang-gun, Busan 619-705, Republic of Korea. Electronic address: nambohye@korea.kr.
authors.forename: Bo-Hye
authors.initials: BH
authors.lastname: Nam
authors.affiliation: Institute of Marine Industry, Department of Marine Biology and Aquaculture, College of Marine Science, Gyeongsang National University, 455, Tongyeong 650-160, Republic of Korea. Electronic address: vinus96@hanmail.net.
authors.forename: Chan-Il
authors.initials: CI
authors.lastname: Park
mesh_terms: D000818:Animals; D037421:Anthozoa / Q000235:genetics* / Q000254:growth & development*; D001483:Base Sequence; D017931:DNA Primers; D020224:Expressed Sequence Tags; D015723:Gene Library; D063990:Gene Ontology; D008969:Molecular Sequence Data; D056910:Republic of Korea; D017422:Sequence Analysis, DNA
chemical_list: D017931:DNA Primers
keywords: EST; Polyp fragment; Settlement; Sinularia notanda; Soft coral