title: Classical swine fever virus induces oxidative stress in swine umbilical vein endothelial cells.
abstract: BACKGROUND
  Classical swine fever virus (CSFV) infection causes significant losses of pigs, which is characterized by hemorrhage, disseminated intravascular coagulation and leucopenia. The swine vascular endothelial cell is a primary target cell for CSFV. The aim of this study was to determine the role of CSFV infection in inducing oxidative stress (OS) in vascular endothelial cells.
  
  
  RESULTS
  We demonstrated that CSFV infection induced oxidative stress in swine umbilical vein endothelial cells (SUVECs), characterized by the induction of reactive oxygen species (ROS) production and the elevations of porcine antioxidant proteins thioredoxin (Trx), peroxiredoxin-6 (PRDX-6) and heme oxygenase-1 (HO-1) expression. Furthermore, cyclooxygenase-2 (COX-2), a pro-inflammatory protein related to oxidative stress, was up-regulated while anti-inflammatory protein peroxisome proliferator-activated receptor-γ (PPAR-γ), an important mediator in vascular functional regulation, was down-regulated in the CSFV infected cells. In addition, antioxidants showed significant inhibitory effects on the CSFV replication, indicating a close relationship between CSFV replication and OS induced in the host cells.
  
  
  CONCLUSIONS
  Our results indicated that CSFV infection induced oxidative stress in SUVECs. These findings provide novel information on the mechanism by which CSFV can alter intracellular events associated with the viral infection.
authors.affiliation: College of Veterinary Medicine, Northwest A & F University, Yangling, Shaanxi, 712100, P.R. China. helei4280546@163.com.|Animal Disease and Public Security Academician Workstation of Henan province, The Key Lab of Animal Disease and Public security, Henan University of Science and Technology, Luoyang, 471003, P.R. China. helei4280546@163.com.
authors.forename: Lei
authors.initials: L
authors.lastname: He
authors.affiliation: College of Veterinary Medicine, Northwest A & F University, Yangling, Shaanxi, 712100, P.R. China. yanmingzhang76@yahoo.com.
authors.forename: Yanming
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: College of Veterinary Medicine, Northwest A & F University, Yangling, Shaanxi, 712100, P.R. China. 1293829973@qq.com.
authors.forename: Yanqin
authors.initials: Y
authors.lastname: Fang
authors.affiliation: College of Veterinary Medicine, Northwest A & F University, Yangling, Shaanxi, 712100, P.R. China. 463932491@qq.com.
authors.forename: Wulong
authors.initials: W
authors.lastname: Liang
authors.affiliation: College of Veterinary Medicine, Northwest A & F University, Yangling, Shaanxi, 712100, P.R. China. 328749233@qq.com.
authors.forename: Jihui
authors.initials: J
authors.lastname: Lin
authors.affiliation: College of Veterinary Medicine, Northwest A & F University, Yangling, Shaanxi, 712100, P.R. China. chming848688@163.com.
authors.forename: Min
authors.initials: M
authors.lastname: Cheng
mesh_terms: D000818:Animals; D006691:Classical Swine Fever / Q000378:metabolism; D006692:Classical Swine Fever Virus / Q000378:metabolism*; D004730:Endothelium, Vascular / Q000166:cytology / Q000378:metabolism / Q000821:virology*; D005434:Flow Cytometry; D051547:Heme Oxygenase-1 / Q000378:metabolism; D008856:Microscopy, Fluorescence; D018384:Oxidative Stress; D054465:Peroxiredoxin VI / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D013552:Swine; D013879:Thioredoxins / Q000378:metabolism; D014471:Umbilical Veins
chemical_list: D017382:Reactive Oxygen Species; D013879:Thioredoxins; D054465:Peroxiredoxin VI; D051547:Heme Oxygenase-1