title: A 3'UTR-associated RNA, FLJ11812 maintains stemness of human embryonic stem cells by targeting miR-4459.
abstract: The 3' untranslated region (UTR)-associated RNAs (uaRNAs) have important roles in various biological processes, especially in development. However, since they overlap with protein-coding mRNAs, uaRNAs are difficult to study by RNA interference techniques. We recently identified a chemical molecule, 3-benzyl-5-((2-nitrophenoxy) methyl)-dihydrofuran-2(3H)-one (3BDO), that could efficiently induce human embryonic stem cells (hESCs) differentiation, and meanwhile selectively and efficiently downregulate the uaRNA FLJ11812. By acting as a competing endogenous RNA, downregulated FLJ11812 by 3BDO further increased miR-4459 level in hESCs. miR-4459 could decrease the expression of its targets, CDC20B and ATG13, and thus altered stemness via cell cycle and autophagy. Our results revealed that FLJ11812 played a key role in maintenance of stemness of hESCs for the first time. The findings provide new clues and a powerful tool for investigating the action mechanism of FLJ11812 in early development.
authors.affiliation: 1 Shandong Provincial Key Laboratory of Animal Cells and Developmental Biology, School of Life Science, Shandong University , Jinan, China .
authors.forename: Wei
authors.initials: W
authors.lastname: Lu
authors.forename: Lei
authors.initials: L
authors.lastname: Han
authors.forename: Le
authors.initials: L
authors.lastname: Su
authors.forename: Jing
authors.initials: J
authors.lastname: Zhao
authors.forename: Yun
authors.initials: Y
authors.lastname: Zhang
authors.forename: ShangLi
authors.initials: S
authors.lastname: Zhang
authors.forename: BaoXiang
authors.initials: B
authors.lastname: Zhao
authors.forename: JunYing
authors.initials: J
authors.lastname: Miao
mesh_terms: D020413:3' Untranslated Regions; D015107:4-Butyrolactone / Q000031:analogs & derivatives / Q000494:pharmacology; D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics / Q000378:metabolism; D000071183:Autophagy-Related Proteins; D064199:Cdc20 Proteins / Q000235:genetics / Q000378:metabolism; D002454:Cell Differentiation; D053595:Embryonic Stem Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D057809:HEK293 Cells; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D062085:RNA, Long Noncoding / Q000235:genetics*
chemical_list: D020413:3' Untranslated Regions; C553471:3-benzyl-5-((2-nitrophenoxy)methyl)dihydrofuran-2(3H)-one; C534291:ATG13 protein, human; D048868:Adaptor Proteins, Signal Transducing; D000071183:Autophagy-Related Proteins; D064199:Cdc20 Proteins; C000603990:MIRN-4456 microRNA, human; D035683:MicroRNAs; D062085:RNA, Long Noncoding; C577488:CDC20 protein, human; D015107:4-Butyrolactone