title: Early diagnosis of blast fungus, Magnaporthe oryzae, in rice plant by using an ultra-sensitive electrically magnetic-controllable electrochemical biosensor.
abstract: As one of the most destructive and widespread disease of rice, Magnaporthe oryzae (also called Magnaporthe grisea) has a significant negative impact on rice production. Therefore, it is still in high demand to develop extremely sensitive and accurate methods for the early diagnosis of Magnaporthe oryzae (M. oryzae). In this study, we developed a novel magnetic-controllable electrochemical biosensor for the ultra sensitive and specific detection of M. oryzae in rice plant by using M. oryzae's chitinases (Mgchi) as biochemical marker and a rice (Oryza sativa) cDNA encoding mannose-binding jacalin-related lectin (Osmbl) as recognition probe. The proposed biosensor combined with the merits of chronoamperometry, electrically magnetic-controllable gold electrode and magnetic beads (MBs)-based palladium nano-particles (PdNPs) catalysis amplification, has an ultra-high sensitivity and specificity for the detection of trace M. oryzae in rice plant. It could be used to detect M. oryzae in rice plant in the initial infection stage (before any symptomatic lesions were observed) to help farmers timely manage the disease. In comparison with previous methods, the proposed method has notable advantages such as higher sensitivity, excellent specificity, short analysis time, robust resistibility to complex matrix and low cost etc. The success in this study provides a reliable approach for the early diagnosis and fast screening of M. oryzae in rice plant.
authors.affiliation: Key Lab of Analysis and Detection for Food Safety of Ministry of Education, Fujian Provincial Key Lab of Analysis and Detection for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou, Fujian 350108, China.
authors.forename: Weijuan
authors.initials: W
authors.lastname: Yang
authors.affiliation: Key Lab of Analysis and Detection for Food Safety of Ministry of Education, Fujian Provincial Key Lab of Analysis and Detection for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou, Fujian 350108, China.
authors.forename: Hongyan
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Key Lab of Analysis and Detection for Food Safety of Ministry of Education, Fujian Provincial Key Lab of Analysis and Detection for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou, Fujian 350108, China.
authors.forename: Mengxue
authors.initials: M
authors.lastname: Li
authors.affiliation: Department of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China.
authors.forename: Zonghua
authors.initials: Z
authors.lastname: Wang
authors.affiliation: School of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Zhou
authors.affiliation: School of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China.
authors.forename: Shihua
authors.initials: S
authors.lastname: Wang
authors.affiliation: Department of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China. Electronic address: lgd@fafu.edu.cn.
authors.forename: Guodong
authors.initials: G
authors.lastname: Lu
authors.affiliation: Key Lab of Analysis and Detection for Food Safety of Ministry of Education, Fujian Provincial Key Lab of Analysis and Detection for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou, Fujian 350108, China; Department of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China. Electronic address: fengfu@fzu.edu.cn.
authors.forename: FengFu
authors.initials: F
authors.lastname: Fu
mesh_terms: D015374:Biosensing Techniques / Q000191:economics / Q000295:instrumentation*; D055664:Electrochemical Techniques / Q000191:economics / Q000295:instrumentation*; D004867:Equipment Design; D020082:Magnaporthe / Q000302:isolation & purification*; D060328:Magnetic Phenomena; D053768:Metal Nanoparticles / Q000737:chemistry / Q000648:ultrastructure; D012275:Oryza / Q000382:microbiology*; D010165:Palladium / Q000737:chemistry; D010935:Plant Diseases / Q000382:microbiology*
chemical_list: D010165:Palladium
keywords: Chitinase; Electrochemical biosensor; Magnaporthe grisea; Magnetic beads; Rice blast