title: Glycopeptide probes for understanding peptide specificity of the folding sensor enzyme UGGT.
abstract: A systematic series of chitobiose-modified pentapeptides with sequence variations of hydrophobic leucine and hydrophilic serine were synthesized. The resulting glycopeptides were used as molecular probes to elucidate aglycon peptide specificity of the glycoprotein-folding sensor enzyme UGGT. Inhibitory experiments with a synthetic fluorescent glyco-substrate and the glycopeptides revealed that UGGT prefers a serine residue directly linked to C-terminal of the N-glycosylation site in its substrate recognition.
authors.affiliation: Department of Materials and Life Science, Seikei University, 3-3-1 Kichijoji-kita, Musashino, Tokyo 180-8633, Japan.
authors.forename: Takaya
authors.initials: T
authors.lastname: Kudo
authors.affiliation: Department of Materials and Life Science, Seikei University, 3-3-1 Kichijoji-kita, Musashino, Tokyo 180-8633, Japan.
authors.forename: Makoto
authors.initials: M
authors.lastname: Hirano
authors.affiliation: Department of Materials and Life Science, Seikei University, 3-3-1 Kichijoji-kita, Musashino, Tokyo 180-8633, Japan.
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Ishihara
authors.affiliation: Department of Materials and Life Science, Seikei University, 3-3-1 Kichijoji-kita, Musashino, Tokyo 180-8633, Japan.
authors.forename: Shun
authors.initials: S
authors.lastname: Shimura
authors.affiliation: Department of Materials and Life Science, Seikei University, 3-3-1 Kichijoji-kita, Musashino, Tokyo 180-8633, Japan. Electronic address: ktotani@st.seikei.ac.jp.
authors.forename: Kiichiro
authors.initials: K
authors.lastname: Totani
mesh_terms: D000595:Amino Acid Sequence; D001896:Boron Compounds / Q000737:chemistry; D004187:Disaccharides / Q000737:chemistry; D005964:Glucosyltransferases / Q000037:antagonists & inhibitors* / Q000378:metabolism; D006020:Glycopeptides / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism*; D057927:Hydrophobic and Hydrophilic Interactions; D015335:Molecular Probes / Q000737:chemistry / Q000378:metabolism*; D011485:Protein Binding; D013379:Substrate Specificity
chemical_list: C095489:4,4-difluoro-4-bora-3a,4a-diaza-s-indacene; D001896:Boron Compounds; D004187:Disaccharides; D006020:Glycopeptides; D015335:Molecular Probes; C032438:chitobiose; D005964:Glucosyltransferases
keywords: Enzyme; Glycopeptide; Inhibitor; Protein folding; Specificity