title: Green synthesis of the silver nanoparticles mediated by pullulan and 6-carboxypullulan.
abstract: Unoxidized and carboxylated pullulan (obtained by pullulan oxidation using TEMPO-sodium hypochlorite-sodium bromide system) have been used as mediators for the silver nanoparticles formation (AgNPs), under environment-friendly conditions: using aqueous solutions, room temperature and notably, by using both mediators as reducing and stabilizing agents. The formation of AgNPs was first screened by measuring the surface plasmon resonance peak in the range of 380-440 nm using UV-vis spectroscopy. The morphology of the synthesized silver nanoparticles was determined by TEM, which indicated that the AgNPs differ on shape and thickness of the polymer shell by varying the silver nitrate concentration, different size and shape of AgNPs was achieved. The presence of elemental silver and the crystalline structure of the AgNPs were confirmed by EDX and XRD analyses. Moreover, the possible functional groups of pullulan (oxidized pullulan) responsible for the reduction and stabilization of AgNPs were evaluated using FT-IR. The results showed that both, pullulan and 6-carboxypullulan could be successfully used as reducing as well as capping agents for the AgNPs synthesis which shows potential antimicrobial activity against Gram positive and Gram negative bacteria.
authors.affiliation: Petru Poni Institute of Macromolecular Chemistry of Romanian Academy, 41 A, Gr. Ghica Voda Alley, 700487 Iasi, Romania. Electronic address: coseris@icmpp.ro.
authors.forename: Sergiu
authors.initials: S
authors.lastname: Coseri
authors.affiliation: Petru Poni Institute of Macromolecular Chemistry of Romanian Academy, 41 A, Gr. Ghica Voda Alley, 700487 Iasi, Romania.
authors.forename: Alina
authors.initials: A
authors.lastname: Spatareanu
authors.affiliation: Petru Poni Institute of Macromolecular Chemistry of Romanian Academy, 41 A, Gr. Ghica Voda Alley, 700487 Iasi, Romania.
authors.forename: Liviu
authors.initials: L
authors.lastname: Sacarescu
authors.affiliation: University of Agricultural Sciences and Veterinary Medicine, Faculty of Veterinary Medicine-UASVM Iaşi, Aleea Mihail Sadoveanu nr.8, 700489 Iaşi, Romania.
authors.forename: Cristina
authors.initials: C
authors.lastname: Rimbu
authors.affiliation: "Gheorghe Asachi" Technical University of Iasi, Faculty of Chemical Engineering and Environmental Protection, 71A Prof. Dr. Docent D. Mangeron Blvd, 700050 Iasi, Romania.
authors.forename: Daniela
authors.initials: D
authors.lastname: Suteu
authors.affiliation: Graz University of Technology, Institute for Chemistry and Technology of Materials, Stremayrgasse 9, 8010 Graz, Austria; University of Maribor, Institute for the Engineering of Materials and Design, Smetanova Ulica 17, 2000 Maribor, Slovenia. Electronic address: stefan.spirk@tugraz.at.
authors.forename: Stefan
authors.initials: S
authors.lastname: Spirk
authors.affiliation: Petru Poni Institute of Macromolecular Chemistry of Romanian Academy, 41 A, Gr. Ghica Voda Alley, 700487 Iasi, Romania.
authors.forename: Valeria
authors.initials: V
authors.lastname: Harabagiu
keywords: 6-Carboxypullulan; Oxidation; Pullulan; Silver nanoparticles; TEMPO