title: Mutations in and Expression of the Tumor Suppressor Gene p53 in Egg-Type Chickens Infected With Subgroup J Avian Leukosis Virus.
abstract: To investigate the molecular mechanisms of the oncogenic effects of avian leukosis virus subgroup J (ALV-J), we examined mutations in and the expression of p53 in the myelocytomas distributed in the liver, spleen, trachea, and bone marrow, as well as in fibrosarcomas in the abdominal cavity and hemangiomas in skin from chickens that were naturally or experimentally infected with ALV-J. Two types of mutations in the p53 gene were detected in myelocytomas of both the experimentally infected and the naturally infected chickens and included point mutations and deletions. Two of the point mutations have not been reported previously. Partial complementary DNA clones with a 122-bp deletion in the p53 gene ORF and a 15-bp deletion in the C-terminus were identified in the myelocytomas. In addition, moderate expression of the mutant p53 protein was detected in the myelocytomas that were distributed in the liver, trachea, spleen, and bone marrow. Mutant p53 protein was not detected in the subcutaneous hemangiomas or in the abdominal fibrosarcomas associated with natural and experimental ALV-J infection, respectively. These results identify mutations associated with abnormal expression of p53 in ALV-J-associated myelocytomas, suggesting a role in tumorigenesis.
authors.affiliation: Division of Avian infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, Heilongjiang, P.R. China College of Animal Husbandry and Veterinary, Liao Ning Medical University, P.R. China College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Taian, P.R. China Liaocheng Vocational and Technical College, Liaocheng, P.R. China.
authors.forename: Q
authors.initials: Q
authors.lastname: Yue
authors.affiliation: Division of Avian infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, Heilongjiang, P.R. China.
authors.forename: G
authors.initials: G
authors.lastname: Yulong
authors.affiliation: Division of Avian infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, Heilongjiang, P.R. China.
authors.forename: Q
authors.initials: Q
authors.lastname: Liting
authors.affiliation: Division of Avian infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, Heilongjiang, P.R. China.
authors.forename: Y
authors.initials: Y
authors.lastname: Shuai
authors.affiliation: Division of Avian infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, Heilongjiang, P.R. China College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Taian, P.R. China.
authors.forename: L
authors.initials: L
authors.lastname: Delong
authors.affiliation: School of Agriculture, Liaocheng University, Liaocheng, P.R. China.
authors.forename: L
authors.initials: L
authors.lastname: Yubao
authors.affiliation: College of Animal Husbandry and Veterinary, Liao Ning Medical University, P.R. China.
authors.forename: J
authors.initials: J
authors.lastname: Lili
authors.affiliation: College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Taian, P.R. China liusid@sdau.edu.cn.
authors.forename: L
authors.initials: L
authors.lastname: Sidang
authors.affiliation: Division of Avian infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, Heilongjiang, P.R. China Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Disease and Zoonoses, Yangzhou, 225009 xmw@hvri.ac.cn.
authors.forename: W
authors.initials: W
authors.lastname: Xiaomei
mesh_terms: D000818:Animals; D001353:Avian Leukosis / Q000150:complications* / Q000821:virology; D001354:Avian Leukosis Virus / Q000302:isolation & purification / Q000472:pathogenicity*; D002645:Chickens / Q000821:virology*; D005260:Female; D006391:Hemangioma / Q000473:pathology / Q000662:veterinary*; D009154:Mutation; D011201:Poultry Diseases / Q000473:pathology* / Q000821:virology; D013047:Specific Pathogen-Free Organisms; D016159:Tumor Suppressor Protein p53 / Q000235:genetics*
chemical_list: D016159:Tumor Suppressor Protein p53
keywords: egg-type chicken; mutation; retroviruses; subgroup J avian leukosis; tumor suppressor gene p53