title: Development of a subunit vaccine containing recombinant Riemerella anatipestifer outer membrane protein A and CpG ODN adjuvant.
abstract: Riemerella anatipestifer, a Gram-negative bacillus, causes septicemia that can result in high mortality for ducklings. In this study, we evaluated the immune response and protective efficacy provided by a subunit vaccine containing recombinant outer membrane protein A (rOmpA) and plasmid constructs containing CpG oligodeoxynucleotides (ODN). Results showed that CpG ODN enhanced both humoral and cell-mediated immunity elicited by rOmpA as early as two weeks after primary immunization. When compared to ducks immunized with rOmpA, ducks immunized with rOmpA+CpG ODN showed higher levels (p<0.05) of antibody titer, T cell proliferation, and percentages of CD4(+) and CD8(+) T cell in peripheral blood mononuclear cells (PBMCs). The relative fold inductions of mRNA expression of Th1-type (IFN-γ and IL-12), and Th2-type (IL-6) cytokines in PBMCs isolated from ducks immunized with rOmpA+CpG ODN were significantly higher than those of the rOmpA group. Homologous challenge result showed that the rOmpA+CpG ODN vaccine reduced the pathological score by 90% in comparison with the saline control. In conclusion, our study found that CpG ODN can enhance both humoral and cellular immunity elicited by a rOmpA vaccine. The rOmpA+CpG ODN vaccine can be further developed as a subunit vaccine against R. anatipestifer.
authors.affiliation: Graduate Institute of Animal Vaccine Technology, National Pingtung University of Science and Technology, 1, Shuefu Road, Neipu, Pingtung 91201, Taiwan.
authors.forename: Chun-Yen
authors.initials: CY
authors.lastname: Chu
authors.affiliation: Graduate Institute of Animal Vaccine Technology, National Pingtung University of Science and Technology, 1, Shuefu Road, Neipu, Pingtung 91201, Taiwan.
authors.forename: Chia-Hui
authors.initials: CH
authors.lastname: Liu
authors.affiliation: Graduate Institute of Animal Vaccine Technology, National Pingtung University of Science and Technology, 1, Shuefu Road, Neipu, Pingtung 91201, Taiwan.
authors.forename: Jhong-Jie
authors.initials: JJ
authors.lastname: Liou
authors.affiliation: Department of Tropical Agriculture and International Cooperation, National Pingtung University of Science and Technology, 1, Shuefu Road, Neipu, Pingtung 91201, Taiwan.
authors.forename: Jai-Wei
authors.initials: JW
authors.lastname: Lee
authors.affiliation: Graduate Institute of Animal Vaccine Technology, National Pingtung University of Science and Technology, 1, Shuefu Road, Neipu, Pingtung 91201, Taiwan. Electronic address: chenglt@mail.npust.edu.tw.
authors.forename: Li-Ting
authors.initials: LT
authors.lastname: Cheng
mesh_terms: D000276:Adjuvants, Immunologic / Q000008:administration & dosage*; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics / Q000276:immunology*; D001428:Bacterial Vaccines / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D049109:Cell Proliferation; D016207:Cytokines / Q000096:biosynthesis; D004372:Ducks; D045826:Flavobacteriaceae Infections / Q000473:pathology / Q000517:prevention & control / Q000662:veterinary*; D020869:Gene Expression Profiling; D009838:Oligodeoxyribonucleotides / Q000008:administration & dosage*; D011201:Poultry Diseases / Q000473:pathology / Q000517:prevention & control*; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology; D060494:Riemerella / Q000235:genetics / Q000276:immunology*; D016896:Treatment Outcome; D022223:Vaccines, Subunit / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology
chemical_list: D000276:Adjuvants, Immunologic; D000907:Antibodies, Bacterial; D001425:Bacterial Outer Membrane Proteins; D001428:Bacterial Vaccines; C408982:CPG-oligonucleotide; D016207:Cytokines; D009838:Oligodeoxyribonucleotides; D011994:Recombinant Proteins; D022223:Vaccines, Subunit; C078167:OMPA outer membrane proteins
keywords: CpG adjuvant; Duck; OmpA; Riemerella anatipestifer; Subunit vaccine