title: Isolation and characterization of four medium-size disintegrins from  the venoms of Central American viperid snakes of the genera  Atropoides, Bothrops, Cerrophidion and Crotalus.
abstract: Four disintegrins were isolated from the venoms of the Central American viperid snakes Atropoides mexicanus (atropoimin), Bothrops asper (bothrasperin), Cerrophidion sasai (sasaimin), and Crotalus simus (simusmin). Purifications were performed by reverse-phase HPLC. The four disintegrins have biochemical characteristics, i.e. molecular mass and location of Cys, which allow their classification within the group of medium-size disintegrins. All of them present the canonical RGD sequence, which determines their interaction with integrins in cell membranes. The disintegrins inhibited ADP and collagen-induced human platelet aggregation, with similar IC50s in the nM range. In addition, disintegrins inhibited the adhesion of an endothelial cell line and a melanoma cell line to the extracellular matrix proteins type I collagen, laminin, fibronectin, and vitronectin, albeit showing variable ability to exert this activity. This study expands the inventory of this family of viperid venom proteins, and reports, for the first time, disintegrins from the venoms of species of the genera Atropoides and Cerrophidion.
authors.forename: Yamileth
authors.initials: Y
authors.lastname: Angulo
authors.forename: Adriana
authors.initials: A
authors.lastname: Castro
authors.forename: Bruno
authors.initials: B
authors.lastname: Lomonte
authors.forename: Alexandra
authors.initials: A
authors.lastname: Rucavado
authors.forename: Julián
authors.initials: J
authors.lastname: Fernández
authors.forename: Juan J
authors.initials: JJ
authors.lastname: Calvete
authors.forename: José María
authors.initials: JM
authors.lastname: Gutiérrez
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D002460:Cell Line / Q000187:drug effects; D003364:Costa Rica; D003435:Crotalid Venoms / Q000737:chemistry; D019483:Disintegrins / Q000737:chemistry* / Q000302:isolation & purification* / Q000494:pharmacology*; D016326:Extracellular Matrix Proteins / Q000378:metabolism; D005353:Fibronectins / Q000378:metabolism; D006801:Humans; D020128:Inhibitory Concentration 50; D007797:Laminin / Q000378:metabolism; D051379:Mice; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009842:Oligopeptides / Q000737:chemistry; D010974:Platelet Aggregation / Q000187:drug effects; D010975:Platelet Aggregation Inhibitors / Q000737:chemistry / Q000494:pharmacology*; D014757:Viper Venoms / Q000737:chemistry*
chemical_list: D003435:Crotalid Venoms; D019483:Disintegrins; D016326:Extracellular Matrix Proteins; D005353:Fibronectins; D007797:Laminin; D009842:Oligopeptides; D010975:Platelet Aggregation Inhibitors; D014757:Viper Venoms; C047981:arginyl-glycyl-aspartic acid