title: Influence of the time of hepatocyte infusion on liver transplantation outcome.
abstract: OBJECTIVE
  Donor-derived hepatocytes infused into recipient rats before liver transplantation can improve the results of liver transplantation in rats. However, the appropriate time when the hepatocytes were infused before transplantation is needed to be explored.
  
  
  METHODS
  All the rats were randomly divided into five groups, the recipient rats were infused with donor-derived hepatocytes at different points of time before transplantation (Group A received the injection one week prior to the transplantation; group B, two weeks prior to the transplantation; group C, three weeks; and group D, four weeks; the control group did not receive hepatocytes infusion). The alanine aminotransferase (ALT), alkaline phosphatase (ALP), and albumin (ALB) levels were tested and the survival time was recorded.
  
  
  RESULTS
  The survival times of recipient rats in group B was the longest among the five groups. The level of serum ALT and ALP in group B were the lowest and the level of ALB was the highest among the five groups.
  
  
  CONCLUSIONS
  In order to achieve the best result following liver transplantation, infusion of hepatocytes two weeks before liver transplantation was determined to be the optimal time.
authors.forename: Chongzhong
authors.initials: C
authors.lastname: Liu
authors.forename: Jiankang
authors.initials: J
authors.lastname: Zhu
authors.forename: Zhendong
authors.initials: Z
authors.lastname: Gao
authors.forename: Min
authors.initials: M
authors.lastname: Zhu
authors.forename: Fengyue
authors.initials: F
authors.lastname: Liu
authors.forename: Mingwei
authors.initials: M
authors.lastname: Zhong
authors.forename: Yadong
authors.initials: Y
authors.lastname: Wang
authors.forename: Sanyuan
authors.initials: S
authors.lastname: Hu
mesh_terms: D000410:Alanine Transaminase / Q000097:blood; D000469:Alkaline Phosphatase / Q000097:blood; D000818:Animals; D015415:Biomarkers / Q000097:blood; D022781:Hepatocytes / Q000637:transplantation*; D016031:Liver Transplantation / Q000009:adverse effects; D008297:Male; D017207:Rats, Sprague-Dawley; D017208:Rats, Wistar; D012709:Serum Albumin / Q000378:metabolism; D013997:Time Factors
chemical_list: D015415:Biomarkers; D012709:Serum Albumin; D000410:Alanine Transaminase; D000469:Alkaline Phosphatase