title: Determination of the lipophilicity (log P o/w) of organic compounds by microemulsion liquid chromatography.
abstract: Four microemulsion liquid chromatography (MELC) systems and one micellar liquid chromatography (MLC) system have been evaluated as potential high-throughput screening platforms capable of modeling the partitioning behaviors of drug compounds in an n-octanol/water system and predicting their lipophilicity (i.e., log P values). The microemulsion mobile phases is consisted of sodium dodecyl sulfate (SDS), butanol, octane, heptanes, octanol and water. A linear solvation energy relationship (LSER)-based method was used to compare the MELC and MLC systems, as well as several other biochemical systems, and to identify the optimal system by comparing their Euclidean distances with the LSER coefficients. The most effective MELC system had a mobile phase consisting of 3.0% (w/w) SDS, 6.0% (w/w) butanol, 0.8% (w/w) octanol, and 90.2% (w/w) water (pH 6.4). The results showed that it gave superior results to the other chromatographic systems in terms of its ability to predict the log P values of drug compounds.
authors.affiliation: Guangdong Pharmaceutical University, Department of Pharmaceutics Analysis, School of Pharmacy, Guangzhou 510006, China.
authors.forename: Liyuan
authors.initials: L
authors.lastname: Xu
authors.affiliation: Guangdong Pharmaceutical University, Department of Traditional Chinese Medicine, School of Traditional Chinese Medicine, Guangzhou 510006, China.
authors.forename: Liangxing
authors.initials: L
authors.lastname: Li
authors.affiliation: Guangdong Pharmaceutical University, Department of Pharmaceutics Analysis, School of Pharmacy, Guangzhou 510006, China.
authors.forename: Jindian
authors.initials: J
authors.lastname: Huang
authors.affiliation: Guangdong Pharmaceutical University, Department of Pharmaceutics Analysis, School of Pharmacy, Guangzhou 510006, China.
authors.forename: Suna
authors.initials: S
authors.lastname: Yu
authors.affiliation: Shenyang Pharmaceutical University, Department of Pharmaceutics, School of Pharmacy, Shenyang 110016, China. Electronic address: wang-jing6312@163.com.
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.affiliation: Guangdong Pharmaceutical University, Department of Pharmaceutics Analysis, School of Pharmacy, Guangzhou 510006, China. Electronic address: 13724117338@163.com.
authors.forename: Ning
authors.initials: N
authors.lastname: Li
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods; D004655:Emulsions / Q000032:analysis* / Q000737:chemistry; D008055:Lipids / Q000032:analysis* / Q000737:chemistry; D000442:Octanols / Q000032:analysis / Q000737:chemistry; D009930:Organic Chemicals / Q000032:analysis* / Q000737:chemistry; D004364:Pharmaceutical Preparations / Q000032:analysis* / Q000737:chemistry
chemical_list: D004655:Emulsions; D008055:Lipids; D000442:Octanols; D009930:Organic Chemicals; D004364:Pharmaceutical Preparations
keywords: Linear solvation energy relationship; Lipophilicity; Microemulsion liquid chromatography; Octanol/water partition coefficient; high-throughput screening