title: Complete genome sequence of the novel Porphyromonadaceae bacterium strain ING2-E5B isolated from a mesophilic lab-scale biogas reactor.
abstract: In this study, the whole genome sequence of the mesophilic, anaerobic Porphyromonadaceae bacterium strain ING2-E5B (LMG 28429, DSM 28696) is reported. The new isolate belongs to the phylum Bacteroidetes and was obtained from a biogas-producing lab-scale completely stirred tank reactor (CSTR) optimized for anaerobic digestion of maize silage in co-fermentation with pig and cattle manure. The genome of strain ING2-E5B contains numerous genes encoding proteins and enzymes involved in the degradation of complex carbohydrates and proteinaceous compounds. Moreover, it possesses genes catalyzing the production of volatile fatty acids. Hence, this bacterium was predicted to be involved in hydrolysis and acidogenesis during anaerobic digestion and biomethanation.
authors.affiliation: Department of Bioengineering, Leibniz Institute for Agricultural Engineering Potsdam-Bornim (ATB), Max-Eyth-Allee 100, 14469 Potsdam, Germany.
authors.forename: Sarah
authors.initials: S
authors.lastname: Hahnke
authors.affiliation: Center for Biotechnology (CeBiTec), Institute for Genome Research and Systems Biology, Bielefeld University, Universitätsstr. 27, 33615 Bielefeld, Germany.
authors.forename: Irena
authors.initials: I
authors.lastname: Maus
authors.affiliation: Center for Biotechnology (CeBiTec), Institute for Genome Research and Systems Biology, Bielefeld University, Universitätsstr. 27, 33615 Bielefeld, Germany.
authors.forename: Daniel
authors.initials: D
authors.lastname: Wibberg
authors.affiliation: Center for Biotechnology (CeBiTec), Institute for Genome Research and Systems Biology, Bielefeld University, Universitätsstr. 27, 33615 Bielefeld, Germany.
authors.forename: Geizecler
authors.initials: G
authors.lastname: Tomazetto
authors.affiliation: Center for Biotechnology (CeBiTec), Institute for Genome Research and Systems Biology, Bielefeld University, Universitätsstr. 27, 33615 Bielefeld, Germany.
authors.forename: Alfred
authors.initials: A
authors.lastname: Pühler
authors.affiliation: Department of Bioengineering, Leibniz Institute for Agricultural Engineering Potsdam-Bornim (ATB), Max-Eyth-Allee 100, 14469 Potsdam, Germany.
authors.forename: Michael
authors.initials: M
authors.lastname: Klocke
authors.affiliation: Center for Biotechnology (CeBiTec), Institute for Genome Research and Systems Biology, Bielefeld University, Universitätsstr. 27, 33615 Bielefeld, Germany. Electronic address: aschluet@cebitec.uni-bielefeld.de.
authors.forename: Andreas
authors.initials: A
authors.lastname: Schlüter
mesh_terms: D000818:Animals; D041963:Bacteroidetes / Q000235:genetics*; D056804:Biofuels; D019149:Bioreactors / Q000382:microbiology; D002417:Cattle; D016680:Genome, Bacterial / Q000235:genetics*; D006868:Hydrolysis; D008372:Manure; D008969:Molecular Sequence Data; D013552:Swine
chemical_list: D056804:Biofuels; D008372:Manure
keywords: Acidogenesis; Bacteroidetes; Biomethanation; Genome; Hydrolysis