title: Recovery of casein-derived peptides with in vitro inhibitory activity of angiotensin converting enzyme (ACE) using aqueous two-phase systems.
abstract: Peptides inhibiting the activity of angiotensin converting enzyme (ACE) were obtained by trypsin-catalyzed hydrolysis of bovine milk casein, performed at 37°C, during 1, 2, 5, 8 and 24h. Results of in vitro inhibitory activity ranged between 13.4% and 78.5%. The highest ACE inhibitory activity was evidenced for hydrolysates obtained after 2h of reaction. Aqueous two-phase systems (ATPS) formed by polyethylene glycol of 1500gmol-1(PEG 1500)+sodium phosphate or potassium phosphates were produced and evaluated, in terms of partition coefficients (K) and extraction yields (y), to recovery the casein hydrolysates at room temperature. In ATPS containing sodium phosphate, the peptides showed a slightly greater affinity toward the bottom salt-rich phase (0.1≤K≤0.9; 5.7%≤y≤47%). In the case of ATPS containing potassium phosphates, these molecules showed substantially greater affinity toward the top polymer-rich phase (137≤K≤266; y≥99%). These results point out extraction using PEG 1500/potassium phosphate ATPS is an efficient technique to recover casein hydrolysates containing ACE inhibitors peptides. Outlined data will be helpful in integrating such unit operation to larger scale processes.
authors.affiliation: Departamento de Tecnologia Rural e Animal, Universidade Estadual do Sudoeste da Bahia, Praça Primavera 40, Bairro Primavera, 45700-000 Itapetinga, BA, Brazil.
authors.forename: Evaldo Cardozo
authors.initials: EC
authors.lastname: de Souza
authors.affiliation: Departamento de Tecnologia de Alimentos, Universidade Federal de Viçosa, Campus Universitário s/n, Centro, 36570-900 Viçosa, MG, Brazil. Electronic address: jcoimbra@ufv.br.
authors.forename: Jane Sélia Dos Reis
authors.initials: JS
authors.lastname: Coimbra
authors.affiliation: Departamento de Tecnologia de Alimentos, Universidade Federal de Viçosa, Campus Universitário s/n, Centro, 36570-900 Viçosa, MG, Brazil.
authors.forename: Eduardo Basílio
authors.initials: EB
authors.lastname: de Oliveira
authors.affiliation: Departamento de Tecnologia Rural e Animal, Universidade Estadual do Sudoeste da Bahia, Praça Primavera 40, Bairro Primavera, 45700-000 Itapetinga, BA, Brazil.
authors.forename: Renata Cristina Ferreira
authors.initials: RC
authors.lastname: Bonomo
keywords: Antihypertensive; Bioseparation; Bovine milk; Phase equilibrium; Protein; Trypsin