title: Adenovirus-mediated NDRG2 inhibits the proliferation of human renal cell carcinoma cell line OS-RC-2 in vitro.
abstract: OBJECTIVE
  To investigate the inhibitory effects of adenovirus-mediated NDRG2 on the proliferation of human renal cell carcinoma cell line OS-RC-2 in vitro.
  
  
  METHODS
  NDRG2 was harvested by RT-PCR, confirmed by DNA sequencing, and then cloned into the eukaryotic expression vector pIRES2-EGFP, which encodes green fluorescent protein (GFP), to construct pIRES2-EGFP-NDRG2 plasmid. OS-RC-2 cells with NDRG2 negative expression were transfected with pIRES2-EGFP-NDRG2 plasmid. The growth of transfected OS-RC-2 cells was observed under light and fluorescence microscopes. After colony-forming cell assays, cell proliferation detection and MTT assays, the growth curves of cells in each group were plotted to investigate the inhibitory effects of adenovirus-mediated NDRG2 on the proliferation of OS-RC-2 cells. Cell cycle was determined by flow cytometry. Confocal laser scanning microscopy showed that NDRG2 protein was specifically located on subcellular organelle.
  
  
  RESULTS
  A eukaryotic expression vector pIRES2-EGFP-NDRG2 was successfully constructed. After NDRG2 transfection, the growth of OS-RC-2 cells was inhibited. Flow cytometry showed that cells were arrested in S phase but the peak of cell apoptosis was not present, and confocal laser scanning microscopy showed that NDRG2 protein was located in mitochondrion.
  
  
  CONCLUSIONS
  NDRG2 can significantly inhibit the proliferation of OS-RC-2 cells in vitro and its protein is specifically expressed in the mitochondrion.
authors.affiliation: Zhangjiagang Hospital of Traditional Chinese Medicine, Shenbingke 215600, China. Electronic address: Qiangsheng660@163.com.
authors.forename: Sheng
authors.initials: S
authors.lastname: Qiang
authors.affiliation: Zhangjiagang Hospital of Traditional Chinese Medicine, Shenbingke 215600, China.
authors.forename: Zhen-Fang
authors.initials: ZF
authors.lastname: Du
authors.affiliation: Zhangjiagang Hospital of Traditional Chinese Medicine, Shenbingke 215600, China.
authors.forename: Min
authors.initials: M
authors.lastname: Huang
keywords: Adenovirus; Inhibition; NDRG2; OS-RC-2; Proliferation