title: Interaction of ochratoxin A with quaternary ammonium beta-cyclodextrin.
abstract: Ochratoxin A (OTA) is a widely spread nephrotoxic food contaminant mycotoxin. Unfortunately, attenuation or prevention of the toxic effects of OTA is still an unresolved problem. Molecular inclusion of OTA by cyclodextrins (CDs) results in complexes with low stability. In the human organism, OTA exists mostly in the dianionic state (OTA(2-)). Therefore, our major goal was to develop a chemically modified cyclodextrin which gives a more stable complex with OTA than the previously published derivatives and which shows stronger preference towards OTA(2-). In our fluorescence spectroscopic study we demonstrate that quaternary ammonium beta-cyclodextrin (QABCD) fulfils both of these requirements. The calculated stability constant of the QABCD-OTA(2-) complex was 28,840 M(-1) (about 200-fold higher than that of the β-CD-OTA(2-) complex). We hypothesize, that QABCD may be a suitable tool for the decontamination of different OTA-contaminated drinks; furthermore, for alleviation of the toxic effects of OTA, such complex formation may reduce its absorption from the intestine.
authors.affiliation: Institute of Laboratory Medicine, University of Pécs, Ifjúság útja 13., Pécs H-7624, Hungary.
authors.forename: Miklós
authors.initials: M
authors.lastname: Poór
authors.affiliation: Department of General and Physical Chemistry, University of Pécs, Ifjúság útja 6., Pécs H-7624, Hungary; János Szentágothai Research Center, Ifjúság útja 20., Pécs H-7624, Hungary.
authors.forename: Sándor
authors.initials: S
authors.lastname: Kunsági-Máté
authors.affiliation: CycloLab Cyclodextrin Research & Development Laboratory, Ltd., Illatos út 7., Budapest H-1097, Hungary.
authors.forename: Lajos
authors.initials: L
authors.lastname: Szente
authors.affiliation: Department of General and Physical Chemistry, University of Pécs, Ifjúság útja 6., Pécs H-7624, Hungary; János Szentágothai Research Center, Ifjúság útja 20., Pécs H-7624, Hungary.
authors.forename: Gergely
authors.initials: G
authors.lastname: Matisz
authors.affiliation: Department of General and Physical Chemistry, University of Pécs, Ifjúság útja 6., Pécs H-7624, Hungary; János Szentágothai Research Center, Ifjúság útja 20., Pécs H-7624, Hungary.
authors.forename: Györgyi
authors.initials: G
authors.lastname: Secenji
authors.affiliation: Department of General and Physical Chemistry, University of Pécs, Ifjúság útja 6., Pécs H-7624, Hungary; János Szentágothai Research Center, Ifjúság útja 20., Pécs H-7624, Hungary.
authors.forename: Zsuzsanna
authors.initials: Z
authors.lastname: Czibulya
authors.affiliation: Institute of Laboratory Medicine, University of Pécs, Ifjúság útja 13., Pécs H-7624, Hungary. Electronic address: koszegit@freemail.hu.
authors.forename: Tamás
authors.initials: T
authors.lastname: Kőszegi
mesh_terms: D000418:Albumins / Q000737:chemistry; D005506:Food Contamination; D006801:Humans; D008274:Magnesium / Q000737:chemistry; D008958:Models, Molecular; D009183:Mycotoxins / Q000737:chemistry*; D009793:Ochratoxins / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D047392:beta-Cyclodextrins / Q000737:chemistry*
chemical_list: D000418:Albumins; D009183:Mycotoxins; D009793:Ochratoxins; D047392:beta-Cyclodextrins; C025589:ochratoxin A; D008274:Magnesium; C031215:betadex
keywords: Cyclodextrin; Detoxification; Fluorescence spectroscopy; Molecular inclusion; Ochratoxin A