title: Peptide imprinted receptors for the determination of the small cell lung cancer associated biomarker progastrin releasing peptide.
abstract: Peptide imprinted polymers were developed for detection of progastrin releasing peptide (ProGRP); a low abundant blood based biomarker for small cell lung cancer. The polymers targeted the proteotypic nona-peptide sequence NLLGLIEAK and were used for selective enrichment of the proteotypic peptide prior to LCMS based quantification. Peptide imprinted polymers with the best affinity characteristics were first identified from a 96-polymer combinatorial library. The effects of functional monomers, crosslinker, porogen, and template on adsorption capacity and selectivity for NLLGLIEAK were investigated and optimized. Ultimately, a solid phase extraction method was developed for highly selective enrichment of the target peptide from tryptic digests.
authors.affiliation: Institute of Environmental Research (INFU) of the Faculty of Chemistry and Chemical Biology, TU Dortmund, Otto-Hahn-Str. 6, 44221 Dortmund, Germany.
authors.forename: Abed Abdel
authors.initials: AA
authors.lastname: Qader
authors.affiliation: Institute of Environmental Research (INFU) of the Faculty of Chemistry and Chemical Biology, TU Dortmund, Otto-Hahn-Str. 6, 44221 Dortmund, Germany.
authors.forename: Javier
authors.initials: J
authors.lastname: Urraca
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Southern Denmark, Campusvej 55, 5230 Odense M, Denmark; Department of Pharmaceutical Chemistry, School of Pharmacy, University of Oslo, Sem Sælands vei 3, N-0316 Oslo, Norway.
authors.forename: Silje Bøen
authors.initials: SB
authors.lastname: Torsetnes
authors.affiliation: Department of Pharmaceutical Chemistry, School of Pharmacy, University of Oslo, Sem Sælands vei 3, N-0316 Oslo, Norway.
authors.forename: Finn
authors.initials: F
authors.lastname: Tønnesen
authors.affiliation: Department of Pharmaceutical Chemistry, School of Pharmacy, University of Oslo, Sem Sælands vei 3, N-0316 Oslo, Norway.
authors.forename: Léon
authors.initials: L
authors.lastname: Reubsaet
authors.affiliation: Institute of Environmental Research (INFU) of the Faculty of Chemistry and Chemical Biology, TU Dortmund, Otto-Hahn-Str. 6, 44221 Dortmund, Germany; Department of Biomedical Sciences, Faculty of Health and Society, Malmö University, SE20506 Malmö, Sweden. Electronic address: borje.sellergren@mah.se.
authors.forename: Börje
authors.initials: B
authors.lastname: Sellergren
mesh_terms: D000327:Adsorption; D000595:Amino Acid Sequence; D014408:Biomarkers, Tumor / Q000032:analysis*; D005755:Gastrins / Q000378:metabolism; D006801:Humans; D008175:Lung Neoplasms / Q000737:chemistry*; D054802:Molecular Imprinting / Q000379:methods*; D010455:Peptides / Q000737:chemistry*; D011498:Protein Precursors / Q000378:metabolism; D055752:Small Cell Lung Carcinoma / Q000737:chemistry*; D052616:Solid Phase Extraction / Q000379:methods*
chemical_list: D014408:Biomarkers, Tumor; D005755:Gastrins; D010455:Peptides; D011498:Protein Precursors; C013706:big gastrin
keywords: Combinatorial synthesis; MIP; Molecularly imprinted polymers; Solid phase extraction