title: Transcription of innate immunity genes and cytokine secretion by canine macrophages resistant or susceptible to intracellular survival of Leishmania infantum.
abstract: In this study we assessed the basal transcription of genes associated with innate immunity (i.e. Nramp1, NOD1, NOD2, TLR1, TLR2, TLR3, TLR4, TLR5, TLR6, TLR7, and TLR9) in canine monocyte-derived macrophages from Leishmania-free dogs. Additionally, secretion of cytokines (IL-10, IL-12, TNF-α and IFN-γ) and nitric oxide in culture supernatants of macrophages with higher or lower resistance to intracellular survival of Leishmania infantum was also measured. Constitutive transcription of TLR9 and NOD2 were negligible; NOD1, TLR1, and TLR7 had low levels of transcription, whereas Nramp1 and TLR2, 3, 4, 5, and 6 had higher levels of constitutive transcription in canine monocyte-derived macrophages. There were no significant differences in transcription between macrophages with higher or lower resistance to intracellular survival of L. infantum. Secretion of TNF-α was higher in more resistant macrophages (designated as resistant) at 24h after infection when compared to less resistant macrophages (designated as susceptible), as well as the secretion of IFN-γ at 72 h post infection. Secretion of IL-10 was lower in resistant macrophages at 24h after infection. No detectable production of nitric oxide was observed. Interestingly, there was a negative correlation between NOD2 transcript levels and intracellular survival of L. infantum in resistant macrophages. This study demonstrated that decreased intracellular survival of L. infantum in canine macrophages was associated with increased production of TNF-α and IFN-γ and decreased production of IL-10; and that constitutive transcription of Nramp1, TLR and NLR does not interfere in intracellular survival of L. infantum.
authors.affiliation: Departamento de Clínica e Cirurgia Veterinárias, Escola de Veterinária, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.
authors.forename: Andréia Pereira
authors.initials: AP
authors.lastname: Turchetti
authors.affiliation: Departamento de Patologia Geral, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.
authors.forename: Luciana Fachini
authors.initials: LF
authors.lastname: da Costa
authors.affiliation: Departamento de Clínica e Cirurgia Veterinárias, Escola de Veterinária, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.
authors.forename: Everton de Lima
authors.initials: Ede L
authors.lastname: Romão
authors.affiliation: Departamento de Parasitologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.
authors.forename: Ricardo Toshio
authors.initials: RT
authors.lastname: Fujiwara
authors.affiliation: Departamento de Patologia Geral, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.
authors.forename: Tatiane Alves
authors.initials: TA
authors.lastname: da Paixão
authors.affiliation: Departamento de Clínica e Cirurgia Veterinárias, Escola de Veterinária, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil. Electronic address: rsantos@vet.ufmg.br.
authors.forename: Renato Lima
authors.initials: RL
authors.lastname: Santos
mesh_terms: D000818:Animals; D016207:Cytokines / Q000096:biosynthesis / Q000502:physiology; D060467:Disease Resistance / Q000235:genetics / Q000276:immunology / Q000502:physiology; D004198:Disease Susceptibility / Q000276:immunology / Q000503:physiopathology / Q000662:veterinary; D004283:Dog Diseases / Q000235:genetics / Q000276:immunology / Q000469:parasitology* / Q000503:physiopathology; D004285:Dogs; D005260:Female; D007113:Immunity, Innate / Q000235:genetics / Q000502:physiology; D018314:Leishmania infantum / Q000276:immunology / Q000502:physiology; D007898:Leishmaniasis, Visceral / Q000276:immunology / Q000503:physiopathology / Q000662:veterinary*; D008264:Macrophages / Q000276:immunology / Q000469:parasitology / Q000502:physiology*; D008297:Male; D009569:Nitric Oxide / Q000378:metabolism; D014158:Transcription, Genetic / Q000276:immunology / Q000502:physiology
chemical_list: D016207:Cytokines; D009569:Nitric Oxide
keywords: Cytokines; Dog; Innate immunity; Leishmania infantum; Macrophage