title: Micro-Raman spectroscopy for monitoring changes in periodontal ligaments and gingival crevicular fluid.
abstract: Micro-Raman Spectroscopy is an efficient method for analyzing biological specimens due to its sensitivity to subtle chemical and structural changes. The aim of this study was to use micro-Raman spectroscopy to analyze chemical and structural changes in periodontal ligament after orthodontic force application and in gingival crevicular fluid in presence of periodontal disease. The biopsy of periodontal ligament samples of premolars extracted for orthodontic reasons and the gingival crevicular fluid samples collected by using absorbent paper cones; were analyzed by micro-Raman spectroscopy. Changes of the secondary protein structure related to different times of orthodontic force application were reported; whereas an increase of carotene was revealed in patients affected by periodontal inflammation.
authors.affiliation: CNR-SPIN, Istituto Superconduttori, Materiali Innovativi e Dispositivi, via Campi Flegrei 34, Pozzuoli 80078, Italy. carlo.camerlingo@spin.cnr.it.
authors.forename: Carlo
authors.initials: C
authors.lastname: Camerlingo
authors.affiliation: Dip. Multidisciplinare di Specialità Medico-Chirurgiche e Odontoiatriche, Seconda Università di Napoli, via L. De Crecchio 6, Napoli 80138, Italy. fabriziadapuzzo@gmail.com.
authors.forename: Fabrizia
authors.initials: F
authors.lastname: d'Apuzzo
authors.affiliation: Dip. Multidisciplinare di Specialità Medico-Chirurgiche e Odontoiatriche, Seconda Università di Napoli, via L. De Crecchio 6, Napoli 80138, Italy. grassiavincenzo@libero.it.
authors.forename: Vincenzo
authors.initials: V
authors.lastname: Grassia
authors.affiliation: Dip. Multidisciplinare di Specialità Medico-Chirurgiche e Odontoiatriche, Seconda Università di Napoli, via L. De Crecchio 6, Napoli 80138, Italy. letizia.perillo@unina2.it.
authors.forename: Letizia
authors.initials: L
authors.lastname: Perillo
authors.affiliation: Dip. di Medicina Sperimentale, Seconda Università di Napoli, S. Maria di Costantinopoli 16, Napoli 80138, Italy. maria.lepore@unina2.it.
authors.forename: Maria
authors.initials: M
authors.lastname: Lepore
mesh_terms: D000293:Adolescent; D000328:Adult; D003799:Dental Stress Analysis; D005260:Female; D005883:Gingival Crevicular Fluid / Q000737:chemistry*; D006801:Humans; D008297:Male; D010513:Periodontal Ligament / Q000737:chemistry*; D017433:Protein Structure, Secondary; D011506:Proteins / Q000032:analysis* / Q000648:ultrastructure*; D013059:Spectrum Analysis, Raman / Q000379:methods*; D014087:Tooth Movement Techniques; D055815:Young Adult
chemical_list: D011506:Proteins