title: Achromobacter denitrificans strain SP1 efficiently remediates di(2-ethylhexyl)phthalate.
abstract: This study describes how Achromobacter denitrificans strain SP1, a novel isolate from heavily plastics-contaminated sewage sludge efficiently consumed the hazardous plasticizer, di(2-ethylhexyl)phthalate (DEHP) as carbon source supplemented in a simple basal salt medium (BSM). Response surface methodology was employed for the statistical optimization of the process parameters such as temperature (32°C), agitation (200 rpm), DEHP concentration (10 mM), time (72 h) and pH (8.0). At these optimized conditions, experimentally observed DEHP degradation was 63%, while the predicted value was 59.2%; and the correlation coefficient between them was 0.998, i.e., highly significant and fit to the predicted model. Employing GC-MS analysis, the degradation pathway was partially deduced with intermediates such as mono(2-ethylhexyl)phthalate and 2-ethyl hexanol. Briefly, this first report describes A. denitrificans strain SP1 as a highly efficient bacterium for completely remediating the hazardous DEHP (10 mM) in 96 h in BSM (50% consumed in 60 h), which offers great potentials for efficiently cleaning the DEHP-contaminated environments such as soil, sediments and water upon its deployment.
authors.affiliation: Enzyme Technology Laboratory, Biotechnology Division, Department of Botany, University of Calicut, Malappuram 673635, Kerala, India.
authors.forename: S
authors.initials: S
authors.lastname: Pradeep
authors.affiliation: Enzyme Technology Laboratory, Biotechnology Division, Department of Botany, University of Calicut, Malappuram 673635, Kerala, India.
authors.forename: M K Sarath
authors.initials: MK
authors.lastname: Josh
authors.affiliation: National Institute for Interdisciplinary Science and Technology, CSIR, Thiruvananthapuram 695019, Kerala, India.
authors.forename: P
authors.initials: P
authors.lastname: Binod
authors.affiliation: Department of Chemistry, Mahatma Gandhi College, University of Kerala, Thiruvananthapuram 695004, Kerala, India.
authors.forename: R Sudha
authors.initials: RS
authors.lastname: Devi
authors.affiliation: Department of Chemistry, Mahatma Gandhi College, University of Kerala, Thiruvananthapuram 695004, Kerala, India.
authors.forename: S
authors.initials: S
authors.lastname: Balachandran
authors.affiliation: United States Department of Agriculture, Agricultural Research Service, Southern Plains Agricultural Research Center, Food and Feed Safety Research Unit, 2881 F&B Road, College Station, TX 77845, USA.
authors.forename: Robin C
authors.initials: RC
authors.lastname: Anderson
authors.affiliation: Enzyme Technology Laboratory, Biotechnology Division, Department of Botany, University of Calicut, Malappuram 673635, Kerala, India. Electronic address: benjamin@uoc.ac.in.
authors.forename: Sailas
authors.initials: S
authors.lastname: Benjamin
mesh_terms: D042441:Achromobacter denitrificans / Q000378:metabolism*; D001673:Biodegradation, Environmental; D004051:Diethylhexyl Phthalate / Q000378:metabolism*; D008401:Gas Chromatography-Mass Spectrometry; D010968:Plasticizers / Q000378:metabolism*; D012989:Soil Pollutants / Q000378:metabolism*; D014874:Water Pollutants, Chemical
chemical_list: D010968:Plasticizers; D012989:Soil Pollutants; D014874:Water Pollutants, Chemical; D004051:Diethylhexyl Phthalate
keywords: Achromobacter denitrificans SP1; Biodegradation; Blood storage bag; DEHP; PVC; Response surface methodology