title: The number of circulating endothelial progenitor cells in healthy individuals--effect of some anthropometric and environmental factors (a pilot study).
abstract: OBJECTIVE
  The aim of the study was the evaluation of the number of circulating endothelial progenitor cells (CEPCs) in healthy people and the assessment of the variability of quantitative of CEPCs after 6 weeks.
  
  
  METHODS
  The study involved 48 healthy individuals; the group consisted of 24 men and 24 women; the mean age of 34. The criterion for the patients' eligibility for the study was the absence of diabetes, thrombosis and cardiovascular diseases such as atherosclerosis, hypertension, and heart failure. Neither did the respondents take any medication that could clearly affect the value of the results. In the whole blood samples the number of circulating endothelial progenitor cells was determined using flow cytometry. During the analysis the fluorescence of 100,000 cells was measured. CEPCs were identified with immunophenotype CD45-, CD31+, CD34+, CD133+.
  
  
  RESULTS
  In the study, the median of the number of circulating endothelial progenitor cells in the whole group was 0.41/μL. There was also recorded an increased number of CEPCs after 6 weeks, as compared to the baseline; the difference was significant. There were no differences in the number of CEPCs between the women and the men. There was found no effect on the number of CEPCs factors such as: smoking, physical activity and alcohol consumption.
  
  
  CONCLUSIONS
  The study showed that in healthy individuals the gender had no essential effect on the number of endothelial progenitor cells. Based on the demographic and lifestyle data acquired, it is difficult to explain the increase number of CEPCs after 6 weeks.
authors.affiliation: Department of Pathophysiology, Nicolaus Copernicus University, Collegium Medicum in Bydgoszcz, Poland. Electronic address: ruszkowska.basia@gmail.com.
authors.forename: Barbara
authors.initials: B
authors.lastname: Ruszkowska-Ciastek
authors.affiliation: Department of Pathophysiology, Nicolaus Copernicus University, Collegium Medicum in Bydgoszcz, Poland.
authors.forename: Alina
authors.initials: A
authors.lastname: Sokup
authors.affiliation: Department of Pathophysiology, Nicolaus Copernicus University, Collegium Medicum in Bydgoszcz, Poland.
authors.forename: Monika
authors.initials: M
authors.lastname: Leszcz
authors.affiliation: Department of Pathophysiology, Nicolaus Copernicus University, Collegium Medicum in Bydgoszcz, Poland.
authors.forename: Ewelina
authors.initials: E
authors.lastname: Drela
authors.affiliation: Department of Pathophysiology, Nicolaus Copernicus University, Collegium Medicum in Bydgoszcz, Poland.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Stankowska
authors.affiliation: Department of Pathophysiology, Nicolaus Copernicus University, Collegium Medicum in Bydgoszcz, Poland.
authors.forename: Joanna
authors.initials: J
authors.lastname: Boinska
authors.affiliation: Faculty of Health Science, University of Humanities and Economics in Włocławek, Poland.
authors.forename: Beata
authors.initials: B
authors.lastname: Haor
authors.affiliation: Department of Neurological and Neurosurgical Nursing, Nicolaus Copernicus University, Collegium Medicum in Bydgoszcz, Poland.
authors.forename: Robert
authors.initials: R
authors.lastname: Ślusarz
authors.affiliation: Department of Pathophysiology, Nicolaus Copernicus University, Collegium Medicum in Bydgoszcz, Poland.
authors.forename: Beata
authors.initials: B
authors.lastname: Lisewska
authors.affiliation: Department of Hematology and Malignant Diseases of Hematopoietic System, Nicolaus Copernicus University in Toruń, Collegium Medicum in Bydgoszcz, Poland.
authors.forename: Grażyna
authors.initials: G
authors.lastname: Gadomska
authors.affiliation: Department of Cardiology and Internal Medicine, Nicolaus Copernicus University, Collegium Medicum in Bydgoszcz, Poland.
authors.forename: Jacek
authors.initials: J
authors.lastname: Kubica
authors.affiliation: Department of Pathophysiology, Nicolaus Copernicus University, Collegium Medicum in Bydgoszcz, Poland.
authors.forename: Danuta
authors.initials: D
authors.lastname: Rość
mesh_terms: D000328:Adult; D000368:Aged; D000428:Alcohol Drinking; D000886:Anthropometry; D066026:Endothelial Progenitor Cells / Q000166:cytology*; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D008019:Life Style; D008297:Male; D008875:Middle Aged; D009043:Motor Activity; D010865:Pilot Projects; D012737:Sex Factors; D012907:Smoking
keywords: Circulating endothelial progenitor cells; Gender; Healthy individuals; Lifestyle