title: The growth factor-like adipokine tartrate-resistant acid phosphatase 5a interacts with the rod G3 domain of adipocyte-produced nidogen-2.
abstract: The adipokine tartrate resistant acid phosphatase (TRAP) 5a isoform exerts a growth factor-effect on pre-adipocytes. This study aimed to identify potential TRAP 5a interacting proteins in pre-adipocytes using pull down assays in combination with mass spectrometry. Nidogen-2, a protein shown to be expressed intracellularly and for secretion by pre-adipocytes, was shown to interact, through its globular G3 domain, with TRAP 5a in vitro. In vivo, TRAP 5a interacted with nidogen-2 in cultured 3T3-L1 mouse pre-adipocytes, as well as with transforming growth factor-β (TGF-β) interacting protein (TRIP-1), which is a protein that has previously been suggested to interact with TRAP in bone. In addition, TRAP 5a and nidogen-2 co-localized in adipose tissue cells in situ. These results indicate that TRAP 5a interacts with nidogen-2 and TRIP-1 in pre-adipocytic cells.
authors.affiliation: Department of Laboratory Medicine, Division of Pathology, Karolinska Institutet, Karolinska University Hospital Huddinge, SE-141 86 Stockholm, Sweden. Electronic address: christina.patlaka@ki.se.
authors.forename: Christina
authors.initials: C
authors.lastname: Patlaka
authors.affiliation: Linköping University, Institute of Technology, Department of Physics, Chemistry and Biology, Linköping University, SE-581 83 Linköping, Sweden. Electronic address: hong.anh.mai86@hotmail.com.
authors.forename: Hong Anh
authors.initials: HA
authors.lastname: Mai
authors.affiliation: Department of Laboratory Medicine, Division of Pathology, Karolinska Institutet, Karolinska University Hospital Huddinge, SE-141 86 Stockholm, Sweden. Electronic address: pernilla.lang@ki.se.
authors.forename: Pernilla
authors.initials: P
authors.lastname: Lång
authors.affiliation: Department of Laboratory Medicine, Division of Pathology, Karolinska Institutet, Karolinska University Hospital Huddinge, SE-141 86 Stockholm, Sweden. Electronic address: goran.andersson@ki.se.
authors.forename: Göran
authors.initials: G
authors.lastname: Andersson
mesh_terms: D016475:3T3 Cells; D017667:Adipocytes / Q000737:chemistry / Q000378:metabolism*; D054392:Adipokines / Q000032:analysis / Q000378:metabolism; D000273:Adipose Tissue / Q000737:chemistry / Q000378:metabolism; D000818:Animals; D002135:Calcium-Binding Proteins; D015815:Cell Adhesion Molecules; D039642:Eukaryotic Initiation Factors / Q000032:analysis / Q000378:metabolism; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000032:analysis / Q000378:metabolism; D008562:Membrane Glycoproteins / Q000032:analysis / Q000378:metabolism*; D051379:Mice; D054730:Protein Interaction Domains and Motifs; D060066:Protein Interaction Maps; D011994:Recombinant Proteins / Q000032:analysis / Q000378:metabolism; D000071681:Tartrate-Resistant Acid Phosphatase / Q000032:analysis / Q000378:metabolism*
chemical_list: D054392:Adipokines; D002135:Calcium-Binding Proteins; D015815:Cell Adhesion Molecules; D039642:Eukaryotic Initiation Factors; D047908:Intracellular Signaling Peptides and Proteins; D008562:Membrane Glycoproteins; C112846:Nid2 protein, mouse; D011994:Recombinant Proteins; C576121:TRIP-1 protein, mouse; D000071681:Tartrate-Resistant Acid Phosphatase
keywords: Acid phosphatase type 5; Extracellular matrix; Growth factor; Nidogen-2; Pre-adipocytes; Tartrate resistant acid phosphatase