title: Development of glucose biosensor based on reconstitution of glucose oxidase onto polymeric redox mediator coated pencil graphite electrodes.
abstract: In this study, a novel glucose biosensor was fabricated by reconstitutional immobilization of glucose oxidase (GOx) onto a poly(glycidyl methacrylate-co-vinylferrocene) (poly(GMA-co-VFc)) film coated pencil graphite electrode (PGE). The amperometric current response of poly(GMA-co-VFc)-GOx to glucose is linear in the concentration range between 1 and 16mM (correlation coefficient of 0.9998) with a detection limit of 2.7μM (S/N=3). Experimental parameters were studied in detail and optimized, including the pH and temperature governing the analytical performance of the biosensor. The stability and reusability of the biosensor as well as its kinetic parameters have also been studied.
authors.affiliation: Department of Genetics and Bioengineering, Faculty of Engineering, Fatih University, B.Cekmece, Istanbul 34500, Turkey.
authors.forename: Muamer
authors.initials: M
authors.lastname: Dervisevic
authors.affiliation: Department of Genetics and Bioengineering, Faculty of Engineering, Fatih University, B.Cekmece, Istanbul 34500, Turkey.
authors.forename: Emre
authors.initials: E
authors.lastname: Cevik
authors.affiliation: Department of Chemistry, Faculty of Arts and Sciences, Fatih University, B.Cekmece, Istanbul 34500, Turkey. Electronic address: msenel@fatih.edu.tr.
authors.forename: Mehmet
authors.initials: M
authors.lastname: Senel
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation*; D001786:Blood Glucose / Q000032:analysis*; D015190:Blood Glucose Self-Monitoring / Q000295:instrumentation*; D055664:Electrochemical Techniques; D004800:Enzymes, Immobilized / Q000378:metabolism*; D004867:Equipment Design; D005296:Ferrous Compounds / Q000737:chemistry*; D005949:Glucose Oxidase / Q000378:metabolism*; D006108:Graphite / Q000737:chemistry*; D006801:Humans; D010084:Oxidation-Reduction; D011108:Polymers; D011109:Polymethacrylic Acids / Q000737:chemistry*; D055550:Protein Stability
chemical_list: D001786:Blood Glucose; D004800:Enzymes, Immobilized; D005296:Ferrous Compounds; D011108:Polymers; D011109:Polymethacrylic Acids; C000599889:poly(glycidyl methacrylate-co-vinylferrocene); D006108:Graphite; D005949:Glucose Oxidase
keywords: Amperometric biosensor; Ferrocene; Glucose; Mediator