title: Novel spectrophotometric determination of flumethasone pivalate and clioquinol in their binary mixture and pharmaceutical formulation.
abstract: This work is concerned with development and validation of three simple, specific, accurate and precise spectrophotometric methods for determination of flumethasone pivalate (FP) and clioquinol (CL) in their binary mixture and ear drops. Method A is a ratio subtraction spectrophotometric one (RSM). Method B is a ratio difference spectrophotometric one (RDSM), while method C is a mean center spectrophotometric one (MCR). The calibration curves are linear over the concentration range of 3-45 μg/mL for FP, and 2-25 μg/mL for CL. The specificity of the developed methods was assessed by analyzing different laboratory prepared mixtures of the FP and CL. The three methods were validated as per ICH guidelines; accuracy, precision and repeatability are found to be within the acceptable limits.
authors.affiliation: Analytical Chemistry Department, Faculty of Pharmacy, Beni-Suef University, Egypt.
authors.forename: Eglal A
authors.initials: EA
authors.lastname: Abdel-Aleem
authors.affiliation: Analytical Chemistry Department, Faculty of Pharmacy, Cairo University, Kasr El-Aini St., 11562 Cairo, Egypt.
authors.forename: Maha A
authors.initials: MA
authors.lastname: Hegazy
authors.affiliation: Analytical Chemistry Department, Faculty of Pharmacy, Beni-Suef University, Egypt.
authors.forename: Nour W
authors.initials: NW
authors.lastname: Sayed
authors.affiliation: Analytical Chemistry Department, Faculty of Pharmacy, Cairo University, Kasr El-Aini St., 11562 Cairo, Egypt.
authors.forename: M
authors.initials: M
authors.lastname: Abdelkawy
authors.affiliation: Analytical Chemistry Department, Faculty of Pharmacy, Beni-Suef University, Egypt. Electronic address: r_magdy26@yahoo.com.
authors.forename: Rehab M
authors.initials: RM
authors.lastname: Abdelfatah
mesh_terms: D000890:Anti-Infective Agents / Q000032:analysis*; D007464:Clioquinol / Q000032:analysis*; D004338:Drug Combinations; D005443:Flumethasone / Q000031:analogs & derivatives* / Q000032:analysis; D005938:Glucocorticoids / Q000032:analysis*; D019999:Pharmaceutical Solutions / Q000737:chemistry*; D015203:Reproducibility of Results; D013053:Spectrophotometry / Q000379:methods*
chemical_list: D000890:Anti-Infective Agents; D004338:Drug Combinations; D005938:Glucocorticoids; D019999:Pharmaceutical Solutions; C005565:flumethasone pivalate; D007464:Clioquinol; D005443:Flumethasone
keywords: Binary mixture; Clioquinol; Flumethasone; Mean centering; Ratio difference; Ratio subtraction