title: Shared-intermediates in the biosynthesis of thio-cofactors: Mechanism and functions of cysteine desulfurases and sulfur acceptors.
abstract: Cysteine desulfurases utilize a PLP-dependent mechanism to catalyze the first step of sulfur mobilization in the biosynthesis of sulfur-containing cofactors. Sulfur activation and integration into thiocofactors involve complex mechanisms and intricate biosynthetic schemes. Cysteine desulfurases catalyze sulfur-transfer reactions from l-cysteine to sulfur acceptor molecules participating in the biosynthesis of thio-cofactors, including Fe-S clusters, thionucleosides, thiamin, biotin, and molybdenum cofactor. The proposed mechanism of cysteine desulfurases involves the PLP-dependent cleavage of the C-S bond from l-cysteine via the formation of a persulfide enzyme intermediate, which is considered the hallmark step in sulfur mobilization. The subsequent sulfur transfer reaction varies with the class of cysteine desulfurase and sulfur acceptor. IscS serves as a mecca for sulfur incorporation into a network of intertwined pathways for the biosynthesis of thio-cofactors. The involvement of a single enzyme interacting with multiple acceptors, the recruitment of shared-intermediates partaking roles in multiple pathways, and the participation of Fe-S enzymes denote the interconnectivity of pathways involving sulfur trafficking. In Bacillus subtilis, the occurrence of multiple cysteine desulfurases partnering with dedicated sulfur acceptors partially deconvolutes the routes of sulfur trafficking and assigns specific roles for these enzymes. Understanding the roles of promiscuous vs. dedicated cysteine desulfurases and their partnership with shared-intermediates in the biosynthesis of thio-cofactors will help to map sulfur transfer events across interconnected pathways and to provide insight into the hierarchy of sulfur incorporation into biomolecules. This article is part of a Special Issue entitled: Fe/S proteins: Analysis, structure, function, biogenesis and diseases.
authors.affiliation: Department of Chemistry, Wake Forest University, Winston-Salem, NC 27106, USA.
authors.forename: Katherine A
authors.initials: KA
authors.lastname: Black
authors.affiliation: Department of Chemistry, Wake Forest University, Winston-Salem, NC 27106, USA. Electronic address: dossanpc@wfu.edu.
authors.forename: Patricia C
authors.initials: PC
authors.lastname: Dos Santos
mesh_terms: D001426:Bacterial Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000378:metabolism; D053898:Biosynthetic Pathways; D013437:Carbon-Sulfur Lyases / Q000737:chemistry / Q000378:metabolism*; D003067:Coenzymes / Q000096:biosynthesis; D003545:Cysteine / Q000378:metabolism*; D007506:Iron-Sulfur Proteins / Q000096:biosynthesis; D008667:Metalloproteins / Q000096:biosynthesis; D008958:Models, Molecular; D000090006:Molybdenum Cofactors; D017434:Protein Structure, Tertiary; D011621:Pteridines; D011732:Pyridoxal Phosphate / Q000378:metabolism*; D013455:Sulfur / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D013437:Carbon-Sulfur Lyases; D003067:Coenzymes; D003545:Cysteine; D007506:Iron-Sulfur Proteins; D008667:Metalloproteins; D000090006:Molybdenum Cofactors; D011621:Pteridines; D011732:Pyridoxal Phosphate; D013455:Sulfur; C022261:molybdopterin; C080546:cysteine desulfurase
keywords: Cysteine desulfurase; Fe–S cluster; IscS; NifS; Persulfide; SufS; Sulfur acceptor; Sulfur transfer; Thio-cofactor; Thionucleoside