title: Dengue virus infection of erythroid precursor cells is modulated by both thalassemia trait status and virus adaptation.
abstract: Dengue is the most significant arthropod borne viral disease worldwide, and infection with the dengue virus causes a wide range of symptoms in humans, including bone marrow suppression. While the target cells of the virus remain poorly characterized, cells of the myeloid lineage have been shown to be important mediators of the disease. This study sought to determine whether erythroid precursor cells were susceptible to dengue virus infection, and whether erythroid cells from thalassemia trait carriers showed any protection against infection. Infection with a laboratory adapted high passage DENV-2 resulted in high levels of infection during certain stages of differentiation, and cells derived from thalassemia trait carriers showed significantly reduced susceptibility to dengue virus infection. Infection with low passage isolates resulted in only scattered cells showing evidence of infection, but high bystander apoptosis that was reduced by both a caspase 8 inhibitor and anti-tumor necrosis factor 1 receptor antibodies.
authors.affiliation: Molecular Pathology Laboratory, Institute of Molecular Biosciences, Mahidol University, Bangkok, Thailand.
authors.forename: Wannapa
authors.initials: W
authors.lastname: Sornjai
authors.affiliation: Molecular Pathology Laboratory, Institute of Molecular Biosciences, Mahidol University, Bangkok, Thailand.
authors.forename: Kornpat
authors.initials: K
authors.lastname: Khungwanmaythawee
authors.affiliation: Thalassemia Research Center, Institute of Molecular Biosciences, Mahidol University, Bangkok, Thailand.
authors.forename: Saovaros
authors.initials: S
authors.lastname: Svasti
authors.affiliation: Thalassemia Research Center, Institute of Molecular Biosciences, Mahidol University, Bangkok, Thailand.
authors.forename: Suthat
authors.initials: S
authors.lastname: Fucharoen
authors.affiliation: Molecular Pathology Laboratory, Institute of Molecular Biosciences, Mahidol University, Bangkok, Thailand.
authors.forename: Phitchayapak
authors.initials: P
authors.lastname: Wintachai
authors.affiliation: Molecular Pathology Laboratory, Institute of Molecular Biosciences, Mahidol University, Bangkok, Thailand.
authors.forename: Sutee
authors.initials: S
authors.lastname: Yoksan
authors.affiliation: Department of Microbiology, Faculty of Science, Mahidol University, Bangkok, Thailand.
authors.forename: Sukathida
authors.initials: S
authors.lastname: Ubol
authors.affiliation: Molecular Pathology Laboratory, Institute of Molecular Biosciences, Mahidol University, Bangkok, Thailand.
authors.forename: Nitwara
authors.initials: N
authors.lastname: Wikan
authors.affiliation: Molecular Pathology Laboratory, Institute of Molecular Biosciences, Mahidol University, Bangkok, Thailand. Electronic address: duncan_r_smith@hotmail.com.
authors.forename: Duncan R
authors.initials: DR
authors.lastname: Smith
mesh_terms: D000222:Adaptation, Physiological; D000818:Animals; D002460:Cell Line; D002646:Chikungunya virus / Q000235:genetics / Q000502:physiology; D002522:Chlorocebus aethiops; D003716:Dengue Virus / Q000235:genetics / Q000502:physiology*; D015672:Erythroid Precursor Cells / Q000821:virology*; D006801:Humans; D008253:Macaca mulatta; D013789:Thalassemia / Q000235:genetics* / Q000378:metabolism; D014709:Vero Cells; D053586:Virus Internalization
keywords: Dengue; Erythroid precursor cells; Thalassemia trait