title: Discrimination of Rhizoma Gastrodiae (Tianma) using 3D synchronous fluorescence spectroscopy coupled with principal component analysis.
abstract: Rhizoma Gastrodiae (Tianma) of different variants and different geographical origins has vital difference in quality and physiological efficacy. This paper focused on the classification and identification of Tianma of six types (two variants from three different geographical origins) using three dimensional synchronous fluorescence spectroscopy (3D-SFS) coupled with principal component analysis (PCA). 3D-SF spectra of aqueous extracts, which were obtained from Tianma of the six types, were measured by a LS-50B luminescence spectrofluorometer. The experimental results showed that the characteristic fluorescent spectral regions of the 3D-SF spectra were similar, while the intensities of characteristic regions are different significantly. Coupled these differences in peak intensities with PCA, Tianma of six types could be discriminated successfully. In conclusion, 3D-SFS coupled with PCA, which has such advantages as effective, specific, rapid, non-polluting, has an edge for discrimination of the similar Chinese herbal medicine. And the proposed methodology is a useful tool to classify and identify Tianma of different variants and different geographical origins.
authors.affiliation: Faculty of Chinese Traditional Medicine, Yunnan University of Traditional Chinese Medicine, Yunnan 650500, PR China.
authors.forename: Qimeng
authors.initials: Q
authors.lastname: Fan
authors.affiliation: Faculty of Life Science and Technology, Kunming University of Science and Technology, Yunnan 650500, PR China.
authors.forename: Chaoyin
authors.initials: C
authors.lastname: Chen
authors.affiliation: Faculty of Chinese Traditional Medicine, Yunnan University of Traditional Chinese Medicine, Yunnan 650500, PR China.
authors.forename: Zaiqiang
authors.initials: Z
authors.lastname: Huang
authors.affiliation: Faculty of Chinese Traditional Medicine, Yunnan University of Traditional Chinese Medicine, Yunnan 650500, PR China.
authors.forename: Chunmei
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Faculty of Life Science and Technology, Kunming University of Science and Technology, Yunnan 650500, PR China.
authors.forename: Pengjuan
authors.initials: P
authors.lastname: Liang
authors.affiliation: Faculty of Chinese Traditional Medicine, Yunnan University of Traditional Chinese Medicine, Yunnan 650500, PR China. Electronic address: zhaoshenglan@163.com.
authors.forename: Shenglan
authors.initials: S
authors.lastname: Zhao
mesh_terms: D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000145:classification; D005240:Feasibility Studies; D021621:Imaging, Three-Dimensional; D025341:Principal Component Analysis; D027343:Rhizome / Q000737:chemistry* / Q000145:classification*; D013050:Spectrometry, Fluorescence / Q000379:methods; D014867:Water / Q000737:chemistry
chemical_list: D004365:Drugs, Chinese Herbal; D014867:Water
keywords: 3D synchronous fluorescence spectroscopy; Discrimination; Principal component analysis; Rhizoma Gastrodiae; Traditional Chinese Medicine