title: A triticale water-deficit-inducible phytocystatin inhibits endogenous cysteine proteinases in vitro.
abstract: Water-deficit is accompanied by an increase in proteolysis. Phytocystatins are plant inhibitors of cysteine proteinases that belong to the papain and legumain family. A cDNA encoding the protein inhibitor TrcC-8 was identified in the vegetative organs of triticale. In response to water-deficit, increases in the mRNA levels of TrcC-8 were observed in leaf and root tissues. Immunoblot analysis indicated that accumulation of the TrcC-8 protein occurred after 72h of water-deficit in the seedlings. Using recombinant protein, inhibitory activity of TrcC-8 against cysteine proteases from triticale and wheat tissues was analyzed. Under water-deficit conditions, there are increases in cysteine proteinase activities in both plant tissues. The cysteine proteinase activities were inhibited by addition of the recombinant TrcC-8 protein. These results suggest a potential role for the triticale phytocystatin in modulating cysteine proteinase activities during water-deficit conditions.
authors.affiliation: Department of Biochemistry, Faculty of Agriculture and Biology, Warsaw University of Life Sciences - SGGW, Nowoursynowska 159, 02-776 Warsaw, Poland. Electronic address: mrs.magdalena.malinowska@gmail.com.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Chojnacka
authors.affiliation: Department of Biochemistry, Faculty of Agriculture and Biology, Warsaw University of Life Sciences - SGGW, Nowoursynowska 159, 02-776 Warsaw, Poland. Electronic address: joanna_szewinska@sggw.pl.
authors.forename: Joanna
authors.initials: J
authors.lastname: Szewińska
authors.affiliation: Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawińskiego 5a, 02-106 Warsaw, Poland. Electronic address: marcinm@ibb.waw.pl.
authors.forename: Marcin
authors.initials: M
authors.lastname: Mielecki
authors.affiliation: Department of Biochemistry, Faculty of Agriculture and Biology, Warsaw University of Life Sciences - SGGW, Nowoursynowska 159, 02-776 Warsaw, Poland. Electronic address: malgorzata_nykiel@sggw.pl.
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Nykiel
authors.affiliation: Hokkaido Agricultural Research Center, National Agriculture and Food Research Organization, Hitsujigaoka 1, Toyohira-ku, Sapporo 062-8555, Japan. Electronic address: rzi@affrc.go.jp.
authors.forename: Ryozo
authors.initials: R
authors.lastname: Imai
authors.affiliation: Department of Biochemistry, Faculty of Agriculture and Biology, Warsaw University of Life Sciences - SGGW, Nowoursynowska 159, 02-776 Warsaw, Poland. Electronic address: wieslaw_bielawski@sggw.pl.
authors.forename: Wiesław
authors.initials: W
authors.lastname: Bielawski
authors.affiliation: Department of Biochemistry, Faculty of Agriculture and Biology, Warsaw University of Life Sciences - SGGW, Nowoursynowska 159, 02-776 Warsaw, Poland. Electronic address: slawomir_orzechowski@sggw.pl.
authors.forename: Sławomir
authors.initials: S
authors.lastname: Orzechowski
mesh_terms: D015891:Cystatins / Q000378:metabolism*; D057056:Cysteine Proteases / Q000378:metabolism*; D055864:Droughts; D002523:Edible Grain / Q000201:enzymology* / Q000235:genetics / Q000502:physiology*; D018506:Gene Expression Regulation, Plant; D015151:Immunoblotting; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D059748:Proteolysis; D013312:Stress, Physiological / Q000235:genetics; D014908:Triticum / Q000378:metabolism; D014867:Water / Q000378:metabolism*
chemical_list: D015891:Cystatins; D010940:Plant Proteins; D014867:Water; D057056:Cysteine Proteases
keywords: Cysteine proteinase; Drought stress; Phytocystatin; Water deficit