title: Characterizing activities of eukaryotic-like protein kinases with atypical catalytic loop motifs from Myxococcus xanthus.
abstract: Myxococcus xanthus has eukaryotic-like protein kinases (EPKs) with different atypical catalytic loop motifs. Seven out of 14 recombinant M. xanthus EPKs containing atypical motifs in the catalytic loop showed protein kinase activity against myelin basic protein and four autophosphorylated EPKs were detected using anti-phosphotyrosine antibody by western blotting.
authors.affiliation: Department of Applied Biological Science, Faculty of Agriculture, Kagawa University, Miki-cho, Kagawa 761-0795, Japan. Electronic address: kimura@ag.kagawa-u.ac.jp.
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Kimura
authors.affiliation: Department of Applied Biological Science, Faculty of Agriculture, Kagawa University, Miki-cho, Kagawa 761-0795, Japan.
authors.forename: Maho
authors.initials: M
authors.lastname: Urata
authors.affiliation: Department of Applied Biological Science, Faculty of Agriculture, Kagawa University, Miki-cho, Kagawa 761-0795, Japan.
authors.forename: Reiko
authors.initials: R
authors.lastname: Okamoto
mesh_terms: D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D055162:Biocatalysis; D015153:Blotting, Western; D020134:Catalytic Domain; D056890:Eukaryota / Q000201:enzymology*; D008969:Molecular Sequence Data; D004676:Myelin Basic Protein / Q000737:chemistry / Q000378:metabolism; D016941:Myxococcus xanthus / Q000201:enzymology* / Q000235:genetics; D010766:Phosphorylation; D019000:Phosphotyrosine / Q000032:analysis / Q000378:metabolism; D011494:Protein Kinases / Q000032:analysis / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins / Q000032:analysis / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D013379:Substrate Specificity
chemical_list: D004676:Myelin Basic Protein; D011994:Recombinant Proteins; D019000:Phosphotyrosine; D011494:Protein Kinases
keywords: Atypical catalytic loop; Dual-specificity kinase; Eukaryotic-like protein kinase; MBP kinase activity; Myxococcus xanthus