title: Identification and expression analysis of cold and freezing stress responsive genes of Brassica oleracea.
abstract: Cold and freezing stress is a major environmental constraint to the production of Brassica crops. Enhancement of tolerance by exploiting cold and freezing tolerance related genes offers the most efficient approach to address this problem. Cold-induced transcriptional profiling is a promising approach to the identification of potential genes related to cold and freezing stress tolerance. In this study, 99 highly expressed genes were identified from a whole genome microarray dataset of Brassica rapa. Blast search analysis of the Brassica oleracea database revealed the corresponding homologous genes. To validate their expression, pre-selected cold tolerant and susceptible cabbage lines were analyzed. Out of 99 BoCRGs, 43 were differentially expressed in response to varying degrees of cold and freezing stress in the contrasting cabbage lines. Among the differentially expressed genes, 18 were highly up-regulated in the tolerant lines, which is consistent with their microarray expression. Additionally, 12 BoCRGs were expressed differentially after cold stress treatment in two contrasting cabbage lines, and BoCRG54, 56, 59, 62, 70, 72 and 99 were predicted to be involved in cold regulatory pathways. Taken together, the cold-responsive genes identified in this study provide additional direction for elucidating the regulatory network of low temperature stress tolerance and developing cold and freezing stress resistant Brassica crops.
authors.affiliation: Department of Horticulture, Sunchon National University, 413 Jungang-ro, Suncheon, Jeonnam 540-950, Republic of Korea.
authors.forename: Nasar Uddin
authors.initials: NU
authors.lastname: Ahmed
authors.affiliation: Department of Horticulture, Sunchon National University, 413 Jungang-ro, Suncheon, Jeonnam 540-950, Republic of Korea.
authors.forename: Hee-Jeong
authors.initials: HJ
authors.lastname: Jung
authors.affiliation: Department of Horticulture, Sunchon National University, 413 Jungang-ro, Suncheon, Jeonnam 540-950, Republic of Korea.
authors.forename: Jong-In
authors.initials: JI
authors.lastname: Park
authors.affiliation: Department of Crop Science, Chungbuk National University, 410 Seongbongro, Heungdokgu, Cheongju 361-763, Republic of Korea.
authors.forename: Yong-Gu
authors.initials: YG
authors.lastname: Cho
authors.affiliation: Department of Biology, College of Biological Sciences and Biotechnology, Chungnam National University, Daejeon 305-764, Republic of Korea.
authors.forename: Yoonkang
authors.initials: Y
authors.lastname: Hur
authors.affiliation: Department of Horticulture, Sunchon National University, 413 Jungang-ro, Suncheon, Jeonnam 540-950, Republic of Korea. Electronic address: nis@sunchon.ac.kr.
authors.forename: Ill-Sup
authors.initials: IS
authors.lastname: Nou
mesh_terms: D001937:Brassica / Q000145:classification / Q000235:genetics* / Q000502:physiology; D003080:Cold Temperature; D020869:Gene Expression Profiling / Q000379:methods*; D018506:Gene Expression Regulation, Plant; D053263:Gene Regulatory Networks; D018745:Genome, Plant; D020411:Oligonucleotide Array Sequence Analysis; D010940:Plant Proteins / Q000235:genetics*; D060888:Real-Time Polymerase Chain Reaction; D013312:Stress, Physiological
chemical_list: D010940:Plant Proteins
keywords: Brassica oleracea; Cold and freezing stress; Expression; Microarray