title: Expression and functions of ASIC1 in the zebrafish retina.
abstract: It has been demonstrated that acid sensing ionic channels (ASICs) are present in the central and peripheral nervous system of mammals, including the retina. However, it remains unclear whether the zebrafish retina also expresses ASICs. In the present study, the expression and distribution of zasic1 were examined in the retina of zebrafish. Both zasic1 mRNA and protein expressions were detected in the adult zebrafish retina. A wide distribution of ASIC1 in zebrafish retina was confirmed using whole mount in situ hybridization and immunohistochemistry study. Acidosis-induced currents in the isolated retinal ganglion cells (RGCs) were also recorded using whole cell patch clamping. Moreover, blockade of ASICs channel significantly reduced the locomotion of larval zebrafish in response to light exposure. In sum, our data demonstrate the presence of ASIC1 and its possible functional relevance in the retina of zebrafish.
authors.affiliation: Department of Neurology, Jiangsu Key Laboratory of Translational Research and Therapy for Neuro-Psycho-Diseases, The Second Affiliated Hospital of Soochow University, Soochow University, Suzhou 215004, China; Institute of Neuroscience, Soochow University, Suzhou 215123, China.
authors.forename: Sha
authors.initials: S
authors.lastname: Liu
authors.affiliation: Department of Neurology, Jiangsu Key Laboratory of Translational Research and Therapy for Neuro-Psycho-Diseases, The Second Affiliated Hospital of Soochow University, Soochow University, Suzhou 215004, China; Institute of Neuroscience, Soochow University, Suzhou 215123, China.
authors.forename: Mei-Xia
authors.initials: MX
authors.lastname: Wang
authors.affiliation: Department of Neurology, Jiangsu Key Laboratory of Translational Research and Therapy for Neuro-Psycho-Diseases, The Second Affiliated Hospital of Soochow University, Soochow University, Suzhou 215004, China; Institute of Neuroscience, Soochow University, Suzhou 215123, China.
authors.forename: Cheng-Jie
authors.initials: CJ
authors.lastname: Mao
authors.affiliation: Department of Neurology, Jiangsu Key Laboratory of Translational Research and Therapy for Neuro-Psycho-Diseases, The Second Affiliated Hospital of Soochow University, Soochow University, Suzhou 215004, China; Institute of Neuroscience, Soochow University, Suzhou 215123, China.
authors.forename: Xiao-Yu
authors.initials: XY
authors.lastname: Cheng
authors.affiliation: Department of Neurology, Jiangsu Key Laboratory of Translational Research and Therapy for Neuro-Psycho-Diseases, The Second Affiliated Hospital of Soochow University, Soochow University, Suzhou 215004, China; Institute of Neuroscience, Soochow University, Suzhou 215123, China.
authors.forename: Chen-Tao
authors.initials: CT
authors.lastname: Wang
authors.affiliation: Center for Circadian Clock, Soochow University, Suzhou, China; School of Biology & Basic Medical Sciences, Medical College, Soochow University, Suzhou 215123, Jiangsu, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Huang
authors.affiliation: Center for Circadian Clock, Soochow University, Suzhou, China; School of Biology & Basic Medical Sciences, Medical College, Soochow University, Suzhou 215123, Jiangsu, China.
authors.forename: Zhao-Min
authors.initials: ZM
authors.lastname: Zhong
authors.affiliation: Institute of Neuroscience, Soochow University, Suzhou 215123, China.
authors.forename: Wei-Dong
authors.initials: WD
authors.lastname: Hu
authors.affiliation: Department of Neurology, Jiangsu Key Laboratory of Translational Research and Therapy for Neuro-Psycho-Diseases, The Second Affiliated Hospital of Soochow University, Soochow University, Suzhou 215004, China; Institute of Neuroscience, Soochow University, Suzhou 215123, China.
authors.forename: Fen
authors.initials: F
authors.lastname: Wang
authors.affiliation: Institute of Neuroscience, Soochow University, Suzhou 215123, China; Department of Pharmacology, School of Pharmaceutical Science, Soochow University, Suzhou 215123, China.
authors.forename: Li-Fang
authors.initials: LF
authors.lastname: Hu
authors.affiliation: Center for Circadian Clock, Soochow University, Suzhou, China; School of Biology & Basic Medical Sciences, Medical College, Soochow University, Suzhou 215123, Jiangsu, China.
authors.forename: Han
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of Neurology, Jiangsu Key Laboratory of Translational Research and Therapy for Neuro-Psycho-Diseases, The Second Affiliated Hospital of Soochow University, Soochow University, Suzhou 215004, China; Institute of Neuroscience, Soochow University, Suzhou 215123, China. Electronic address: liucf@suda.edu.cn.
authors.forename: Chun-Feng
authors.initials: CF
authors.lastname: Liu
mesh_terms: D062565:Acid Sensing Ion Channels / Q000235:genetics / Q000502:physiology*; D000818:Animals; D018507:Gene Expression Regulation, Developmental; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D007814:Larva; D008027:Light; D008856:Microscopy, Fluorescence; D009043:Motor Activity; D018408:Patch-Clamp Techniques; D012333:RNA, Messenger / Q000378:metabolism; D012160:Retina / Q000378:metabolism*; D012165:Retinal Ganglion Cells / Q000166:cytology; D015027:Zebrafish / Q000235:genetics* / Q000502:physiology; D029961:Zebrafish Proteins / Q000235:genetics / Q000502:physiology*
chemical_list: D062565:Acid Sensing Ion Channels; D012333:RNA, Messenger; D029961:Zebrafish Proteins
keywords: Acid sensing ionic channels; Retina; Zebrafish