title: Amino acid sequence homologies in the hard keratins of birds and reptiles, and their implications for molecular structure and physical properties.
abstract: Avian and reptilian epidermal appendages such as feathers, claws and scales exhibit a filament-matrix texture. Previous studies have established that both components reside within the same single-chain molecule. In the present study the homology in a wide range of aligned sequences is used to gain insights into the structure and function of the molecular segments associated with the filament and with the matrix. The notion that all molecules contain a β-rich 34-residue segment associated with the framework of the filament is reinforced by the present study. In addition, the residues involved in the polymerization of the molecules to form filaments are identified. In the Archosaurs (birds, crocodiles and turtles), and the Squamates (snakes and lizards) segments rich in glycine and tyrosine can be identified in the C-terminal domain. In Rhynocephalians (tuataras) and Squamates a similar segment is inserted at a specific point in the N-terminal domain. In some Archosaurian appendages (both avian and reptilian) segments rich in charged residues and cysteine are found in the N-terminal domain. The likely effect of these segments will be to soften the tissue without compromising its insolubility. The structure and role of the various molecular segments identified in this study and the way in which they might manifest themselves in terms of the physical properties of the particular epidermal appendage in which they appear are also discussed.
authors.affiliation: Institute of Fundamental Sciences, Massey University, Private Bag 11-222, Palmerston North, New Zealand.
authors.forename: R D Bruce
authors.initials: RD
authors.lastname: Fraser
authors.affiliation: Institute of Fundamental Sciences, Massey University, Private Bag 11-222, Palmerston North, New Zealand; Riddet Institute, Massey University, Private Bag 11-222, Palmerston North, New Zealand. Electronic address: d.parry@massey.ac.nz.
authors.forename: David A D
authors.initials: DA
authors.lastname: Parry
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001717:Birds / Q000378:metabolism*; D004817:Epidermis / Q000378:metabolism; D005241:Feathers / Q000378:metabolism; D007633:Keratins / Q000378:metabolism*; D012104:Reptiles / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid
chemical_list: D007633:Keratins
keywords: Archosaurs; Feather; Rhynocephalians; Scale and claw sequences; Squamates; β-Keratins