title: Upregulation of HO-1 Attenuates LPS-Stimulated Proinflammatory Responses Through Downregulation of p38 Signaling Pathways in Rat Ovary.
abstract: Heme oxygenase-1 (HO-1) plays a key role in the regulation of the inflammatory response. However, the specific mechanism underlying the anti-inflammatory role of HO-1 in the reproductive system is unclear. The aim of this study was to evaluate the role of the mechanism of HO-1 in the regulation of the inflammatory response stimulated by lipopolysaccharide (LPS) in rat ovary. LPS-stimulated inflammatory models were established. Rats were pretreated with HO-1 activator (hemin) or inhibitor (ZnPP) before LPS stimulation, and we evaluated the expression of HO-1 by real-time PCR and Western blot. The messenger RNA (mRNA) expression and secretion of IL-1β and IL-6 in rat ovary were analyzed using real-time PCR and ELISA. In addition, we also analyzed the p38 and p-p38 protein expression in the ovary. Our results demonstrate that HO-1 is an anti-inflammatory factor in LPS-stimulated ovary, which regulates the inflammatory response through downregulation of p38 signaling pathways in rat ovary.
authors.affiliation: College of Animal Science and Technology, Nanjing Agricultural University, 210095, Nanjing, People's Republic of China, lilian@njau.edu.cn.
authors.forename: Lian
authors.initials: L
authors.lastname: Li
authors.forename: Juan
authors.initials: J
authors.lastname: Tang
authors.forename: Yu
authors.initials: Y
authors.lastname: Sun
authors.forename: Jie
authors.initials: J
authors.lastname: Wu
authors.forename: Pan
authors.initials: P
authors.lastname: Yu
authors.forename: Genlin
authors.initials: G
authors.lastname: Wang
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000378:metabolism*; D005260:Female; D051547:Heme Oxygenase-1 / Q000037:antagonists & inhibitors / Q000096:biosynthesis / Q000378:metabolism*; D006427:Hemin / Q000494:pharmacology; D007249:Inflammation / Q000139:chemically induced / Q000276:immunology / Q000378:metabolism*; D053583:Interleukin-1beta / Q000096:biosynthesis / Q000378:metabolism; D015850:Interleukin-6 / Q000096:biosynthesis / Q000378:metabolism; D008070:Lipopolysaccharides; D020935:MAP Kinase Signaling System / Q000187:drug effects; D010053:Ovary / Q000378:metabolism*; D018384:Oxidative Stress; D011524:Protoporphyrins / Q000494:pharmacology; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000378:metabolism; D015854:Up-Regulation; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism*
chemical_list: D000893:Anti-Inflammatory Agents; C000594089:IL1B protein, rat; D053583:Interleukin-1beta; D015850:Interleukin-6; D008070:Lipopolysaccharides; D011524:Protoporphyrins; C017803:zinc protoporphyrin; D006427:Hemin; D051547:Heme Oxygenase-1; D013482:Superoxide Dismutase; D048051:p38 Mitogen-Activated Protein Kinases