title: Overexpression of Late Embryogenesis Abundant 14 enhances Arabidopsis salt stress tolerance.
abstract: Late embryogenesis abundant (LEA) proteins are implicated in various abiotic stresses in higher plants. In this study, we identified a LEA protein from Arabidopsis thaliana, AtLEA14, which was ubiquitously expressed in different tissues and remarkably induced with increased duration of salt treatment. Subcellular distribution analysis demonstrated that AtLEA14 was mainly localized in the cytoplasm. Transgenic Arabidopsis and yeast overexpressing AtLEA14 all exhibited enhanced tolerance to high salinity. The transcripts of salt stress-responsive marker genes (COR15a, KIN1, RD29B and ERD10) were overactivated in AtLEA14 overexpressing lines compared with those in wild type plants under normal or salt stress conditions. In vivo and in vitro analysis showed that AtLEA14 could effectively stabilize AtPP2-B11, an important E3 ligase. These results suggested that AtLEA14 had important protective functions under salt stress conditions in Arabidopsis.
authors.affiliation: State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Taian, Shandong 271018, PR China. Electronic address: jfj.5566@163.com.
authors.forename: Fengjuan
authors.initials: F
authors.lastname: Jia
authors.affiliation: State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Taian, Shandong 271018, PR China. Electronic address: zisexanwu@163.com.
authors.forename: Shengdong
authors.initials: S
authors.lastname: Qi
authors.affiliation: State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Taian, Shandong 271018, PR China. Electronic address: 332453593@qq.com.
authors.forename: Hui
authors.initials: H
authors.lastname: Li
authors.affiliation: State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Taian, Shandong 271018, PR China. Electronic address: banbaokezhan@163.com.
authors.forename: Pu
authors.initials: P
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Taian, Shandong 271018, PR China. Electronic address: lpcsdau@163.com.
authors.forename: Pengcheng
authors.initials: P
authors.lastname: Li
authors.affiliation: State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Taian, Shandong 271018, PR China. Electronic address: cawu@sdau.edu.cn.
authors.forename: Changai
authors.initials: C
authors.lastname: Wu
authors.affiliation: State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Taian, Shandong 271018, PR China. Electronic address: cczheng@sdau.edu.cn.
authors.forename: Chengchao
authors.initials: C
authors.lastname: Zheng
authors.affiliation: State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Taian, Shandong 271018, PR China. Electronic address: jghuang@sdau.edu.cn.
authors.forename: Jinguang
authors.initials: J
authors.lastname: Huang
mesh_terms: D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics*; D020869:Gene Expression Profiling; D010940:Plant Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D055049:Salt Tolerance / Q000187:drug effects / Q000235:genetics*; D012965:Sodium Chloride / Q000494:pharmacology; D013312:Stress, Physiological / Q000187:drug effects
chemical_list: D010940:Plant Proteins; D012333:RNA, Messenger; C066246:late embryogenesis abundant protein, plant; D012965:Sodium Chloride
keywords: Arabidopsis thaliana; AtLEA14; AtPP2-B11; Salt stress