title: Structure of an arabinogalactan from the edible tropical fruit tamarillo (Solanum betaceum) and its antinociceptive activity.
abstract: A structural characterization of polysaccharides obtained by aqueous extraction of ripe pulp of the edible exotic tropical fruit named tamarillo (Solanum betaceum) was carried out. After fractionation by freeze-thaw and α-amylase treatments, a fraction containing a mixture of highly-methoxylated homogalacturonan and of arabinogalactan was obtained. A degree of methylesterification (DE) of 71% and a degree of acetylation (DA) of 1.3% was determined by (1)H NMR spectroscopy and spectrophotometric quantification, respectively. A type I arabinogalactan was purified via Fehling precipitation and ultrafiltration through 50 kDa (cut-off) membrane. Its chemical structure was performed by sugar composition, HPSEC, methylation, carboxy-reduction and (13)C NMR spectroscopy analysis. Intraperitoneal administration of the arabinogalactan did not reduce the nociception induced by intraplantar injection of 2.5% formalin in mice, but significantly reduced the number of abdominal constrictions induced by 0.6% acetic acid, indicating that fraction has an antinociceptive effect on the visceral inflammatory pain model.
authors.affiliation: Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, CP 19.046, CEP 81.531-980 Curitiba, PR, Brazil.
authors.forename: Georgia Erdmann
authors.initials: GE
authors.lastname: do Nascimento
authors.affiliation: Departamento de Farmacologia, Universidade Federal do Paraná, CEP 81.531-980 Curitiba, PR, Brazil.
authors.forename: Claudia Rita
authors.initials: CR
authors.lastname: Corso
authors.affiliation: Departamento de Farmacologia, Universidade Federal do Paraná, CEP 81.531-980 Curitiba, PR, Brazil.
authors.forename: Maria Fernanda de Paula
authors.initials: MF
authors.lastname: Werner
authors.affiliation: Departamento de Farmacologia, Universidade Federal do Paraná, CEP 81.531-980 Curitiba, PR, Brazil.
authors.forename: Cristiane Hatsuko
authors.initials: CH
authors.lastname: Baggio
authors.affiliation: Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, CP 19.046, CEP 81.531-980 Curitiba, PR, Brazil.
authors.forename: Marcello
authors.initials: M
authors.lastname: Iacomini
authors.affiliation: Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, CP 19.046, CEP 81.531-980 Curitiba, PR, Brazil. Electronic address: lucimaramcc@ufpr.br.
authors.forename: Lucimara M C
authors.initials: LM
authors.lastname: Cordeiro
mesh_terms: D019342:Acetic Acid; D000700:Analgesics / Q000737:chemistry / Q000302:isolation & purification / Q000627:therapeutic use; D000818:Animals; D005260:Female; D005557:Formaldehyde; D005638:Fruit / Q000737:chemistry*; D005685:Galactans / Q000737:chemistry / Q000302:isolation & purification / Q000627:therapeutic use; D008745:Methylation; D051379:Mice; D015394:Molecular Structure; D008970:Molecular Weight; D009005:Monosaccharides / Q000032:analysis; D010146:Pain / Q000139:chemically induced / Q000188:drug therapy*; D008517:Phytotherapy; D032322:Solanum
chemical_list: D000700:Analgesics; D005685:Galactans; D009005:Monosaccharides; D005557:Formaldehyde; D019342:Acetic Acid; C005653:arabinogalactan
keywords: Antinociceptive effect; Highly-methoxylated homogalacturonan; Solanum betaceum; Tamarillo; Type I Arabinogalactan