title: Development and characterization of a cationic lipid nanocarrier as non-viral vector for gene therapy.
abstract: The aim of the present work was to produce a cationic solid lipid nanoparticle (SLN) as non-viral vector for protein delivery. Cationic SLN were produced by double emulsion method, composed of softisan(®) 100, cetyltrimethylammonium bromide (CTAB), Tween(®) 80, Span(®) 80, glycerol and lipoid(®) S75 loading insulin as model protein. The formulation was characterized in terms of mean hydrodynamic diameter (z-ave), polydispersity index (PI), zeta potential (ZP), stability during storage time, stability after lyophilization, effect of toxicity and transfection ability in HeLa cells, in vitro release profile and morphology. SLN were stable for 30days and showed minimal changes in their physicochemical properties after lyophilization. The particles exhibited a relatively slow release, spherical morphology and were able to transfect HeLa cells, but toxicity remained an obstacle. Results suggest that SLN are nevertheless promising for delivery of proteins or nucleic acids for gene therapy.
authors.affiliation: Department of Biotechnological Processes, School of Engineering Chemical, University of Campinas-Unicamp, Campinas 13083-970, SP, Brazil; Faculty of Health Sciences, Fernando Pessoa University (UFP-FCS), Rua Carlos da Maia, 296, 4200-150 Porto, Portugal.
authors.forename: Patrícia
authors.initials: P
authors.lastname: Severino
authors.affiliation: Laboratory of Genetics and Molecular Analysis, Molecular Biology and Genetic Engineering Center, State University of Campinas, Campinas, SP, Brazil.
authors.forename: Marcelo
authors.initials: M
authors.lastname: Szymanski
authors.affiliation: Laboratory of Genetics and Molecular Analysis, Molecular Biology and Genetic Engineering Center, State University of Campinas, Campinas, SP, Brazil.
authors.forename: Marianna
authors.initials: M
authors.lastname: Favaro
authors.affiliation: Laboratory of Genetics and Molecular Analysis, Molecular Biology and Genetic Engineering Center, State University of Campinas, Campinas, SP, Brazil.
authors.forename: Adriano R
authors.initials: AR
authors.lastname: Azzoni
authors.affiliation: Laboratory for Development and Evaluation of Bioactive Substance, Sorocaba University, UNISO, Sorocaba, SP, Brazil.
authors.forename: Marco V
authors.initials: MV
authors.lastname: Chaud
authors.affiliation: Department of Biotechnological Processes, School of Engineering Chemical, University of Campinas-Unicamp, Campinas 13083-970, SP, Brazil.
authors.forename: Maria Helena A
authors.initials: MH
authors.lastname: Santana
authors.affiliation: Department of Biology and Environment, University of Trás-os-Montes e Alto Douro (UTAD), Quinta de Prados, 5001-801 Vila Real, Portugal; Centre for Research and Technology of Agro-Environmental and Biological Sciences (CITAB-UTAD), Quinta de Prados, 5001-801 Vila Real, Portugal.
authors.forename: Amélia M
authors.initials: AM
authors.lastname: Silva
authors.affiliation: Faculty of Health Sciences, Fernando Pessoa University (UFP-FCS), Rua Carlos da Maia, 296, 4200-150 Porto, Portugal; Faculty of Pharmacy, University of Coimbra (FFUC), Pólo das Ciências da Saúde, Azinhaga de Santa Comba, 3000-548 Coimbra, Portugal. Electronic address: ebsouto@ff.uc.pt.
authors.forename: Eliana B
authors.initials: EB
authors.lastname: Souto
mesh_terms: D000077286:Cetrimonium; D002593:Cetrimonium Compounds; D004247:DNA / Q000008:administration & dosage* / Q000737:chemistry*; D004337:Drug Carriers / Q000737:chemistry; D005786:Gene Expression Regulation; D015316:Genetic Therapy / Q000379:methods*; D005990:Glycerol; D006367:HeLa Cells; D006601:Hexoses; D006801:Humans; D008055:Lipids / Q000737:chemistry*; D008422:Materials Testing; D053758:Nanoparticles; D011136:Polysorbates; D014162:Transfection / Q000379:methods*
chemical_list: D002593:Cetrimonium Compounds; D004337:Drug Carriers; D006601:Hexoses; D008055:Lipids; D011136:Polysorbates; C018665:sorbitan monooleate; D004247:DNA; D005990:Glycerol; C000595885:softisan 100; D000077286:Cetrimonium
keywords: Gene delivery; Nanotechnology; Non-viral vectors; Proteins; Solid lipid nanoparticles