title: Overcoming the solubility problem in E. coli: available approaches for recombinant protein production.
abstract: Despite the importance of recombinant protein production in academy and industrial fields, many issues concerning the expression of soluble and homogeneous product are still unsolved. Although several strategies were developed to overcome these obstacles, at present there is no magic bullet that can be applied for all cases. Indeed, several key expression parameters need to be evaluated for each protein. Among the different hosts for protein expression, Escherichia coli is by far the most widely used. In this chapter, we review many of the different tools employed to circumvent protein insolubility problems.
authors.affiliation: Recombinant Protein Unit, Institut Pasteur de Montevideo, Mataojo 2020, CP, 11400, Montevideo, Uruguay.
authors.forename: Agustín
authors.initials: A
authors.lastname: Correa
authors.forename: Pablo
authors.initials: P
authors.lastname: Oppezzo
mesh_terms: D000818:Animals; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism*; D006801:Humans; D011994:Recombinant Proteins / Q000235:genetics* / Q000378:metabolism*; D012995:Solubility
chemical_list: D011994:Recombinant Proteins