title: Transcriptome analysis of the salivary glands of Nephotettix cincticeps (Uhler).
abstract: The green rice leafhopper (GRH), Nephotettix cincticeps, is one of the most important pests of rice in temperate Asian countries. GRH, a vascular feeder, secretes watery and gelling saliva in the process of feeding on phloem and xylem sap. It is known that GRH saliva contains several bioactive proteins, including enzymes such as laccase and beta-glucosidase. In this study, we performed transcriptome analysis of salivary glands of GRH using Illumina paired-end sequencing. Of 51,788 assembled contigs, 16,017 (30.9%) showed significant similarity to known proteins in the NCBI nr database, while 34,978 (67.5%) could not be annotated by similarity search, Pfam, or gene ontology (GO). Contigs (905) with predicted signal peptides and no putative transmembrane domains are suggested to represent secreted protein coding genes. Among the 76 most highly expressed putative secretory protein contigs, 68 transcripts were found to be salivary gland-specific or at least -dominant, but not expressed in stomach or Malpighian tubules. However, 45 of the 68 transcripts were unknown proteins. These findings suggest that most of the GRH transcripts encoding secreted proteins expressed in salivary glands are species and/or tissue specific. Our results provide a fundamental list of genes involved in GRH-Poaceae host plant interactions including successful feeding and plant pathogen transmission.
authors.affiliation: National Institute of Agrobiological Sciences, Owashi 1-2, Tsukuba, Ibaraki 305-8634, Japan. Electronic address: matsumt@affrc.go.jp.
authors.forename: Yukiko
authors.initials: Y
authors.lastname: Matsumoto
authors.affiliation: National Institute of Agrobiological Sciences, Owashi 1-2, Tsukuba, Ibaraki 305-8634, Japan. Electronic address: suetsugu@affrc.go.jo.
authors.forename: Yoshitaka
authors.initials: Y
authors.lastname: Suetsugu
authors.affiliation: National Institute of Agrobiological Sciences, Kobuchizawa 6585, Hokuto, Yamanashi 408-0044, Japan. Electronic address: nakamura@affrc.go.jp.
authors.forename: Masatoshi
authors.initials: M
authors.lastname: Nakamura
authors.affiliation: National Institute of Agrobiological Sciences, Owashi 1-2, Tsukuba, Ibaraki 305-8634, Japan. Electronic address: hatto@affrc.go.jp.
authors.forename: Makoto
authors.initials: M
authors.lastname: Hattori
mesh_terms: D000818:Animals; D005260:Female; D020869:Gene Expression Profiling; D006430:Hemiptera / Q000235:genetics* / Q000378:metabolism; D009928:Organ Specificity; D060888:Real-Time Polymerase Chain Reaction; D012469:Salivary Glands / Q000378:metabolism; D059467:Transcriptome
keywords: Green rice leafhopper; Illumina HiSeq 2000; Nephotettix cincticeps; Salivary glands; Transcriptome