title: Examination of bioenergetic function in the inner mitochondrial membrane peptidase 2-like (Immp2l) mutant mice.
abstract: Inner mitochondrial membrane peptidase 2-like (IMMP2L) protein is a mitochondrial inner membrane peptidase that cleaves the signal peptide sequences of cytochrome c1 (CYC1) and mitochondrial glycerol phosphate dehydrogenase (GPD2). Immp2l mutant mice show infertility and early signs of aging. It is unclear whether mitochondrial respiratory deficiency underlies this phenotype. Here we show that the intermediate forms of GPD2 and CYC1 have normal expression levels and enzymatic function in Immp2l mutants. Mitochondrial respiration is not diminished in isolated mitochondria and cells from mutant mice. Our data suggest that respiratory deficiency is not the cause of the observed Immp2l mutant phenotypes.
authors.affiliation: Section on Gerontology and Geriatric Medicine, Wake Forest University Health Sciences, Department of Internal Medicine, Medical Center Boulevard, Winston-Salem, NC 27157, USA.
authors.forename: Manish S
authors.initials: MS
authors.lastname: Bharadwaj
authors.affiliation: Wake Forest Institute for Regenerative Medicine, Wake Forest University Health Sciences, Medical Center Boulevard, Winston-Salem, NC 27157, USA.
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: Section on Gerontology and Geriatric Medicine, Wake Forest University Health Sciences, Department of Internal Medicine, Medical Center Boulevard, Winston-Salem, NC 27157, USA.
authors.forename: Anthony J
authors.initials: AJ
authors.lastname: Molina
authors.affiliation: Wake Forest Institute for Regenerative Medicine, Wake Forest University Health Sciences, Medical Center Boulevard, Winston-Salem, NC 27157, USA.
authors.forename: Tracy
authors.initials: T
authors.lastname: Criswell
authors.affiliation: Wake Forest Institute for Regenerative Medicine, Wake Forest University Health Sciences, Medical Center Boulevard, Winston-Salem, NC 27157, USA. Electronic address: blu@wakehealth.edu.
authors.forename: Baisong
authors.initials: B
authors.lastname: Lu
mesh_terms: D000818:Animals; D010450:Endopeptidases / Q000235:genetics / Q000378:metabolism*; D004734:Energy Metabolism; D051379:Mice; D008817:Mice, Mutant Strains; D008928:Mitochondria / Q000235:genetics / Q000378:metabolism*; D051336:Mitochondrial Membranes / Q000378:metabolism*; D024101:Mitochondrial Proteins / Q000235:genetics / Q000378:metabolism*; D009154:Mutation; D010101:Oxygen Consumption
chemical_list: D024101:Mitochondrial Proteins; D010450:Endopeptidases; C568497:inner mitochondrial membrane peptidase 2-like protein, mouse
keywords: Complex III; Cytochrome c1; GPD2; Immp2l; Mitochondrial respiration