title: Nanocrystals of zeolite act as enhanced sensing interface for biosensing of leviteracetum.
abstract: In present work, nanocrystals of zeolites were employed as sensing interface for augmented electrochemical sensing of antiepileptic drug, that is, leviteracetum (LEV). Electrochemical sensing method was developed by depositing nanocrystals of zeolites and horseradish peroxidase (nanocrys zeolites-HRP) onto indium tin oxide (ITO)-coated glass surface for LEV detection. Various stages of biosensor fabrication were characterized by Transmission electron microscopy, X-ray diffraction, scanning electron microscopy, and electrochemical impedance spectroscopy. The fabricated nanocrys zeolites-HRP-modified sensor exhibited wide linear range (10-500 μM) and a low detection limit of 0.01 μM. The biosensor also showed a short response time (within 2 s). In addition, the biosensor exhibited high reproducibility, good storage stability, and anti-interference ability. The applicability of the nanocrys zeolites-HRP-modified sensor is to determine LEV level in spiked serum samples.
authors.affiliation: Amity Institute of Nanotechnology, AMITY University, Noida, UP, India.
authors.forename: Jagriti
authors.initials: J
authors.lastname: Narang
authors.forename: Nidhi
authors.initials: N
authors.lastname: Chauhan
authors.forename: Nitesh
authors.initials: N
authors.lastname: Malhotra
authors.forename: C S
authors.initials: CS
authors.lastname: Pundir
mesh_terms: D000927:Anticonvulsants / Q000032:analysis* / Q000737:chemistry; D015374:Biosensing Techniques; D002626:Chemistry, Pharmaceutical; D018360:Crystallography, X-Ray; D058266:Dielectric Spectroscopy; D055664:Electrochemical Techniques; D000077287:Levetiracetam; D057230:Limit of Detection; D016014:Linear Models; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles; D036103:Nanotechnology; D010889:Piracetam / Q000031:analogs & derivatives* / Q000032:analysis / Q000737:chemistry; D015203:Reproducibility of Results; D013499:Surface Properties; D013678:Technology, Pharmaceutical / Q000379:methods*; D013997:Time Factors; D017641:Zeolites / Q000737:chemistry*
chemical_list: D000927:Anticonvulsants; D017641:Zeolites; D000077287:Levetiracetam; D010889:Piracetam
keywords: ITO electrode; X-ray diffractometry; bioanalysis; enzymology; horseradish peroxidase; leviteracetum; nanocrystals of zeolites; nanoparticles; physical characterization