title: An evaluation of genotoxicity in human neuronal-type cells subjected to oxidative stress under an extremely low frequency pulsed magnetic field.
abstract: The possible genotoxicity of extremely low frequency magnetic field (ELF-MF) exposure is still a controversial topic. The most of the reported data suggests that it alone does not affect DNA integrity, but several recent reports have suggested that sinusoidal ELF-MF may increase the effect of known genotoxic agents. Only a few studies deal with non sinusoidal ELF-MF, including pulsed magnetic field (PMF), which are produced by several devices. The aim of this study is to investigate whether PMF exposure can interfere with DNA damage and repair in the presence of a genotoxic oxidative agent in neuronal type cells. To this purpose gamma-H2AX foci formation, which is a sensitive marker of DNA double strand breaks (DSB), was investigated at different points of time (1, 24, 48, 72h) after the H2O2 treatment (300μM for 1h) under PMF exposure (1mT, 50Hz) in human neuroblastoma BE(2)C cells. Moreover, cytotoxicity evaluation, by MTT assay and cell cycle analysis, was performed at various points of time after the treatment. Taken together, results suggest that PMF exposure does not interfere with genotoxicity and cytotoxicity induced by oxidative stress.
authors.affiliation: Department of Biology, Geology, and Environmental Sciences, University of Bologna, via Selmi 3, 40126 Bologna, Italy; Interdepartmental Center "L.Galvani", University of Bologna, via Berti Pichat 6/2, 40127 Bologna, Italy.
authors.forename: Gianfranco
authors.initials: G
authors.lastname: Giorgi
authors.affiliation: Department of Experimental, Diagnostic and Specialty Medicine, University of Bologna, via Massarenti 9, 40138 Bologna, Italy.
authors.forename: Mariangela
authors.initials: M
authors.lastname: Lecciso
authors.affiliation: Department of Experimental, Diagnostic and Specialty Medicine, University of Bologna, Via S. Giacomo 12, 40126 Bologna, Italy; Interdepartmental Center "L.Galvani", University of Bologna, via Berti Pichat 6/2, 40127 Bologna, Italy.
authors.forename: Miriam
authors.initials: M
authors.lastname: Capri
authors.affiliation: Institute for Biomedical Aging Research, University of Innsbruck, Rennweg 10, A-6020 Innsbruck.
authors.forename: Stella
authors.initials: S
authors.lastname: Lukas Yani
authors.affiliation: Department of Physics and Astronomy, University of Bologna, via Berti Pichat 6/2, 40127 Bologna, Italy; Interdepartmental Center "L.Galvani", University of Bologna, via Berti Pichat 6/2, 40127 Bologna, Italy.
authors.forename: Angela
authors.initials: A
authors.lastname: Virelli
authors.affiliation: Department of Physics and Astronomy, University of Bologna, via Berti Pichat 6/2, 40127 Bologna, Italy; Interdepartmental Center "L.Galvani", University of Bologna, via Berti Pichat 6/2, 40127 Bologna, Italy.
authors.forename: Ferdinando
authors.initials: F
authors.lastname: Bersani
authors.affiliation: Department of Pharmacy and Biotechnology, University of Bologna, via Selmi 3, 40126 Bologna, Italy; Interdepartmental Center "L.Galvani", University of Bologna, via Berti Pichat 6/2, 40127 Bologna, Italy. Electronic address: brunella.delre@unibo.it.
authors.forename: Brunella
authors.initials: B
authors.lastname: Del Re
mesh_terms: D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D053960:DNA Breaks; D004574:Electromagnetic Fields / Q000009:adverse effects*; D006657:Histones / Q000378:metabolism; D006801:Humans; D006861:Hydrogen Peroxide / Q000008:administration & dosage / Q000633:toxicity*; D009152:Mutagenicity Tests; D009447:Neuroblastoma / Q000473:pathology; D018384:Oxidative Stress / Q000187:drug effects*
chemical_list: C468783:H2AX protein, human; D006657:Histones; D006861:Hydrogen Peroxide
keywords: Gamma-H2AX foci; Genotoxicity; Magnetic field; Neuroblastoma; Oxidative stress