title: Light modulation of biomass and macromolecular composition of the diatom Skeletonema marinoi.
abstract: The biochemical profile and growth of the coastal diatom Skeletonema marinoi was investigated under four different daily blue light doses (sinusoidal light peaking at 88, 130, 250 and 450 μmol photons m(−2) s(−1), respectively). Ability of cells to regulate the light energy input caused alterations in growth and different biosynthetic pathways. The light saturation index for photosynthesis (E(k)), which governs the photoacclimative processes, ranged between 250 and 300 μmol photons m(−2) s(−1). Cells that were adapted to low light (<E(k)) enhanced their carotenoid, lipid and protein contents and lowered carbohydrate content, and vice versa under high light (≥E(k)). Variations in fatty acid, pigment and amino acid compositions were a result of light adaptation. Our data show that light is a potent factor for manipulating biomass synthesis in microalgae, such as diatoms for microalgal biotechnology.
authors.forename: Raghu
authors.initials: R
authors.lastname: Chandrasekaran
authors.forename: Lucia
authors.initials: L
authors.lastname: Barra
authors.forename: Sara
authors.initials: S
authors.lastname: Carillo
authors.forename: Tonino
authors.initials: T
authors.lastname: Caruso
authors.forename: Maria Michela
authors.initials: MM
authors.lastname: Corsaro
authors.forename: Fabrizio
authors.initials: F
authors.lastname: Dal Piaz
authors.forename: Giulia
authors.initials: G
authors.lastname: Graziani
authors.forename: Federico
authors.initials: F
authors.lastname: Corato
authors.forename: Debora
authors.initials: D
authors.lastname: Pepe
authors.forename: Alessandro
authors.initials: A
authors.lastname: Manfredonia
authors.forename: Ida
authors.initials: I
authors.lastname: Orefice
authors.forename: Alexander V
authors.initials: AV
authors.lastname: Ruban
authors.forename: Christophe
authors.initials: C
authors.lastname: Brunet
mesh_terms: D000596:Amino Acids / Q000378:metabolism; D018533:Biomass; D050260:Carbohydrate Metabolism / Q000528:radiation effects; D002338:Carotenoids / Q000378:metabolism; D017377:Diatoms / Q000254:growth & development / Q000378:metabolism / Q000528:radiation effects*; D008027:Light; D050356:Lipid Metabolism / Q000528:radiation effects; D010788:Photosynthesis / Q000528:radiation effects; D011506:Proteins / Q000378:metabolism; D012313:RNA / Q000378:metabolism
chemical_list: D000596:Amino Acids; D011506:Proteins; D002338:Carotenoids; D012313:RNA