title: Microcalorimetric assays for measuring cell growth and metabolic activity: methodology and applications.
abstract: Isothermal microcalorimetry measures the heat released or consumed by physical or chemical processes. Metabolic activity releases heat that can be measured. However the calorimetric signal can be difficult for new users to interpret. This paper compares microcalorimetry to other techniques and reviews its application to different fields where microbiological activity is important. We also describe different ways to analyze the data and translate it into meaningful (micro)biological equivalents. This paper aims at providing non-specialist reader the tools to understand how isothermal microcalorimetry can be used for microbiological applications.
authors.affiliation: Department of Urology, University Hospital Basel, Basel, Switzerland; Laboratory of Biomechanics and Biocalorimetry (LOB2), University of Basel, Basel, Switzerland. Electronic address: Olivier.braissant@unibas.ch.
authors.forename: O
authors.initials: O
authors.lastname: Braissant
authors.affiliation: Department of Urology, University Hospital Basel, Basel, Switzerland.
authors.forename: A
authors.initials: A
authors.lastname: Bachmann
authors.affiliation: Department of Urology, University Hospital Basel, Basel, Switzerland; Laboratory of Biomechanics and Biocalorimetry (LOB2), University of Basel, Basel, Switzerland.
authors.forename: G
authors.initials: G
authors.lastname: Bonkat
mesh_terms: D001419:Bacteria / Q000254:growth & development* / Q000378:metabolism; D002151:Calorimetry / Q000379:methods*; D006358:Hot Temperature
keywords: Analytical method; Bacteria; Fungi; Isothermal microcalorimetry; Microbial activity; Microbial growth