title: Effect of surfactant on phenanthrene metabolic kinetics by Citrobacter sp. SA01.
abstract: To attain a better understanding of the effects of surfactants on the metabolic kinetics of hydrophobic organic compounds, the biodegradation of phenanthrene by Citrobacter sp. SA01 was investigated in a batch experiment containing Tween 80, sodium dodecyl benzene sulfonate and liquid mineral salt medium. The Monod model was modified to effectively describe the partition, phenanthrene biodegradation and biopolymer production. The results showed that Tween 80 and sodium dodecyl benzene sulfonate (each at 50mg/L) enhanced phenanthrene metabolism and poly-β-hydroxybutyrate production as indicated by the increasing amounts of intermediates (by 17.2% to 47.9%), and percentages of poly-β-hydroxybutyrate (by 107.3% and 33.1%) within the cell dry weight when compared to their absence. The modified Monod model was capable of predicting microbial growth, phenanthrene depletion and biopolymer production. Furthermore, the Monod kinetic coefficients were largely determined by the surfactant-enhanced partition, suggesting that partitioning is a critical process in surfactant-enhanced bioremediation of hydrophobic organic compounds.
authors.affiliation: Department of Environmental Science, Zhejiang University, Hangzhou 310058, China; Zhejiang Provincial Key Laboratory of Organic Pollution Process Control, Hangzhou 310058, China; Zhejiang Yuying College, Hangzhou 310018, China. Electronic address: lf6220@126.com.
authors.forename: Feng
authors.initials: F
authors.lastname: Li
authors.affiliation: Department of Environmental Science, Zhejiang University, Hangzhou 310058, China; Zhejiang Provincial Key Laboratory of Organic Pollution Process Control, Hangzhou 310058, China. Electronic address: zlz@zju.edu.cn.
authors.forename: Lizhong
authors.initials: L
authors.lastname: Zhu
authors.affiliation: Department of Environmental Science, Zhejiang University, Hangzhou 310058, China; Zhejiang Provincial Key Laboratory of Organic Pollution Process Control, Hangzhou 310058, China.
authors.forename: Dong
authors.initials: D
authors.lastname: Zhang
mesh_terms: D002954:Citrobacter / Q000378:metabolism*; D008401:Gas Chromatography-Mass Spectrometry; D007700:Kinetics; D010616:Phenanthrenes / Q000378:metabolism*; D013501:Surface-Active Agents / Q000737:chemistry*
chemical_list: D010616:Phenanthrenes; D013501:Surface-Active Agents
keywords: Biodegradation; Citrobacter sp.; Kinetics; Phenanthrene; Surfactant