title: Isolating phagosomes from tissue culture cells.
abstract: Phagocytosis is the process by which receptors at the plasma membrane are used to engulf a particle such as a bacterium, parasite, or dead cell. Phagosomes can be isolated from tissue culture cells by various centrifugation methods, including the use of differential density gradients or sucrose step gradients, but these methods are time-consuming or otherwise difficult. We describe here a protocol that avoids centrifugation and relies instead on the uptake of magnetic beads to rapidly isolate the phagosomal compartment from tissue culture cells.
authors.affiliation: Centre for Immunology and Infection, Hull York Medical School and Department of Biology, University of York, York YO10 5DD, United Kingdom.
authors.forename: Paul R
authors.initials: PR
authors.lastname: Pryor
authors.affiliation: Centre for Immunology and Infection, Hull York Medical School and Department of Biology, University of York, York YO10 5DD, United Kingdom.
authors.forename: Adam P
authors.initials: AP
authors.lastname: Rofe
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D051379:Mice; D010587:Phagocytosis; D010588:Phagosomes / Q000378:metabolism*; D046509:Tissue Culture Techniques / Q000379:methods*