title: Wood mimetic hydrogel beads for enzyme immobilization.
abstract: Wood component-based composite hydrogels have potential applications in biomedical fields owing to their low cost, biodegradability, and biocompatibility. The controllable properties of wood mimetic composites containing three major wood components are useful for enzyme immobilization. Here, lipase from Candida rugosa was entrapped in wood mimetic beads containing cellulose, xylan, and lignin by dissolving wood components with lipase in [Emim][Ac], followed by reconstitution. Lipase entrapped in cellulose/xylan/lignin beads in a 5:3:2 ratio showed the highest activity; this ratio is very similar to that in natural wood. The lipase entrapped in various wood mimetic beads showed increased thermal and pH stability. The half-life times of lipase entrapped in cellulose/alkali lignin hydrogel were 31- and 82-times higher than those of free lipase during incubation under denaturing conditions of high temperature and low pH, respectively. Owing to their biocompatibility, biodegradability, and controllable properties, wood mimetic hydrogel beads can be used to immobilize various enzymes for applications in the biomedical, bioelectronic, and biocatalytic fields.
authors.affiliation: Department of Microbial Engineering, Konkuk University, Seoul 143-701, South Korea.
authors.forename: Saerom
authors.initials: S
authors.lastname: Park
authors.affiliation: Department of Microbial Engineering, Konkuk University, Seoul 143-701, South Korea.
authors.forename: Sung Hee
authors.initials: SH
authors.lastname: Kim
authors.affiliation: Department of Chemical and Biochemical Engineering, Dongguk University-Seoul, Seoul 100-715, South Korea.
authors.forename: Keehoon
authors.initials: K
authors.lastname: Won
authors.affiliation: Department of Forest Sciences and Research Institute for Agriculture and Life Science, Seoul National University, Seoul 151-921, South Korea.
authors.forename: Joon Weon
authors.initials: JW
authors.lastname: Choi
authors.affiliation: Department of Chemical Engineering, Kwangwoon University, Seoul 139-701, South Korea.
authors.forename: Yong Hwan
authors.initials: YH
authors.lastname: Kim
authors.affiliation: Department of Microbial Engineering, Konkuk University, Seoul 143-701, South Korea.
authors.forename: Hyung Joo
authors.initials: HJ
authors.lastname: Kim
authors.affiliation: Department of Microbial Engineering, Konkuk University, Seoul 143-701, South Korea.
authors.forename: Yung-Hun
authors.initials: YH
authors.lastname: Yang
authors.affiliation: Department of Microbial Engineering, Konkuk University, Seoul 143-701, South Korea. Electronic address: sanghlee@konkuk.ac.kr.
authors.forename: Sang Hyun
authors.initials: SH
authors.lastname: Lee
mesh_terms: D040761:Biomimetic Materials / Q000737:chemistry*; D002175:Candida / Q000201:enzymology; D002482:Cellulose / Q000737:chemistry; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000737:chemistry* / Q000378:metabolism*; D020100:Hydrogels / Q000737:chemistry*; D008031:Lignin / Q000737:chemistry; D008049:Lipase / Q000737:chemistry / Q000378:metabolism; D008863:Microspheres; D014934:Wood / Q000737:chemistry*; D014990:Xylans / Q000737:chemistry
chemical_list: D004800:Enzymes, Immobilized; D020100:Hydrogels; D014990:Xylans; D002482:Cellulose; D008031:Lignin; D008049:Lipase
keywords: Composite; Hydrogel; Immobilization; Ionic liquid; Lipase; Wood