title: [Anti-erythrocyte and anti-HLA immunization in hemoglobinopathies].
abstract: OBJECTIVE
  Evaluate the anti-erythrocyte and anti-HLA immunization rates in hemoglobinopathies.
  
  
  METHODS
  Cross-sectional study (October 2009-March 2010) on 83 patients followed for hemoglobinopathies. The irregular antibodies research is realized by two techniques: indirect Coombs and enzymatic technique on gel cards. The search for anti-HLA class I antibodies is done by complement dependent lymphocytotoxicity.
  
  
  RESULTS
  The mean age was 30 years (14-64 years), the sex ratio M/F is 0.84. Our series included 42 cases of sickle cell disease (29 homozygous sickle cell anemia and 13 sickle-thalassemia) and 41 cases of thalassemia syndromes (26 major and 15 intermediate). The anti-erythrocyte alloimmunization rate is 10.84% without difference between thalassemia syndromes and sickle cell disease. The autoimmunization rate (22.89%) is higher in thalassemia syndromes (41.46%) than in the sickle cell disease (7.14%) (P<0.001). The anti-HLA immunization rate is 31.6% without difference between thalassemia syndromes and sickle cell disease. The young age, transfusion at a young age and the total number of transfusions are the factors that increase the risk of anti-erythrocyte autoimmunization. No clinicobiological parameter does influence the anti-erythrocyte and anti-HLA alloimmunization. There is no significant association between anti-erythrocyte and anti-HLA immunization.
  
  
  CONCLUSIONS
  The erythrocyte and anti-HLA anti-immunization rates are high in our series. Preventive strategy is needed to ensure optimal blood safety.
authors.affiliation: Faculté de médecine de Tunis, université Tunis El Manar, rue Jbel Lakhdhar, Tunis, Tunisie. Electronic address: naouel-bsalah@voila.fr.
authors.forename: N
authors.initials: N
authors.lastname: Ben Salah
authors.affiliation: Faculté de médecine de Tunis, université Tunis El Manar, rue Jbel Lakhdhar, Tunis, Tunisie.
authors.forename: W
authors.initials: W
authors.lastname: El Borgi
authors.affiliation: Faculté de médecine de Tunis, université Tunis El Manar, rue Jbel Lakhdhar, Tunis, Tunisie.
authors.forename: F
authors.initials: F
authors.lastname: Ben Lakhal
authors.affiliation: Faculté de médecine de Tunis, université Tunis El Manar, rue Jbel Lakhdhar, Tunis, Tunisie.
authors.forename: M
authors.initials: M
authors.lastname: Ben Mansour
authors.affiliation: Faculté de médecine de Tunis, université Tunis El Manar, rue Jbel Lakhdhar, Tunis, Tunisie.
authors.forename: E
authors.initials: E
authors.lastname: Gouider
authors.affiliation: Faculté de médecine de Tunis, université Tunis El Manar, rue Jbel Lakhdhar, Tunis, Tunisie.
authors.forename: Y
authors.initials: Y
authors.lastname: Gorgi
authors.affiliation: Faculté de médecine de Tunis, université Tunis El Manar, rue Jbel Lakhdhar, Tunis, Tunisie.
authors.forename: R
authors.initials: R
authors.lastname: Bardi
authors.affiliation: Faculté de médecine de Tunis, université Tunis El Manar, rue Jbel Lakhdhar, Tunis, Tunisie.
authors.forename: B
authors.initials: B
authors.lastname: Zoueri
authors.affiliation: Faculté de médecine de Tunis, université Tunis El Manar, rue Jbel Lakhdhar, Tunis, Tunisie.
authors.forename: R
authors.initials: R
authors.lastname: Hafsia
mesh_terms: D000293:Adolescent; D000328:Adult; D000367:Age Factors; D001323:Autoantibodies / Q000097:blood; D003165:Complement System Proteins / Q000276:immunology; D003298:Coombs Test; D003430:Cross-Sectional Studies; D004912:Erythrocytes / Q000276:immunology*; D005260:Female; D006680:HLA Antigens / Q000276:immunology*; D006453:Hemoglobinopathies / Q000276:immunology*; D006801:Humans; D007114:Immunization; D007518:Isoantibodies / Q000097:blood; D008297:Male; D008875:Middle Aged; D055815:Young Adult
chemical_list: D001323:Autoantibodies; D006680:HLA Antigens; D007518:Isoantibodies; D003165:Complement System Proteins
keywords: Allo-anticorps; Alloantibody; Auto-anticorps; Autoantibody; Hemoglobinopathy; Hémoglobinopathies; Immunisation; Immunization; Transfusion