title: Quantification of 3-n-butylphthalide in beagle plasma samples by supercritical fluid chromatography with triple quadruple mass spectrometry and its application to an oral bioavailability study.
abstract: A high-throughput, rapid, sensitive, environmentally friendly, and economical supercritical fluid chromatography with triple quadruple mass spectrometry method was established and validated for the first time to determine a cerebral stroke treatment drug named 3-n-butylphthalide in dog plasma. Plasma samples were prepared by protein precipitation with methanol and the analytes were eluted on an ACQUITY UPC(2TM) HSS-C(18) SB column (3 × 100 mm, 1.8 μm) maintained at 50°C. The mobile phase comprised supercritical carbon dioxide/methanol (90:10, v/v) at a flow rate of 1.5 mL/min, the compensation solvent was methanol at a flow rate of 0.2 mL/min and the total run time was 1.5 min per sample. The detection was carried out on a tandem mass spectrometer with an electrospray ionization source. Calibration curves were linear over the concentration range of 1.02-1021.00 ng/mL (r(2) ≥ 0.993) with the lower limit of quantification of 1.02 ng/mL. The intra- and inter-day precision values were below 15% and the accuracy was from 97.90 to 103.70% at all quality control levels. The method was suitable for a pharmacokinetic study of 3-n-butylphthalide in beagle dogs.
authors.affiliation: Department of Pharmaceutical Analysis, School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, PR China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Li
authors.forename: Longshan
authors.initials: L
authors.lastname: Zhao
authors.forename: Xiaoting
authors.initials: X
authors.lastname: Li
authors.forename: Bei
authors.initials: B
authors.lastname: Guo
authors.forename: Juanhang
authors.initials: J
authors.lastname: Zhao
authors.forename: Xianglin
authors.initials: X
authors.lastname: Wang
authors.forename: Tianhong
authors.initials: T
authors.lastname: Zhang
mesh_terms: D000818:Animals; D001572:Benzofurans / Q000008:administration & dosage / Q000097:blood* / Q000493:pharmacokinetics*; D001682:Biological Availability; D025924:Chromatography, Supercritical Fluid / Q000379:methods*; D004285:Dogs; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000032:analysis / Q000493:pharmacokinetics*; D008297:Male; D013058:Mass Spectrometry / Q000379:methods*
chemical_list: D001572:Benzofurans; D004365:Drugs, Chinese Herbal; C027125:3-n-butylphthalide
keywords: Beagle plasma/3-n-Butylphthalide; Oral bioavailability; Pharmacokinetic studies; Supercritical fluid chromatography