title: Transcellular transport characteristics of huperzine alone or in combination with ginkgolide B across Caco-2 and Madin-Darby canine kidney cell monolayer.
abstract: OBJECTIVE
  To study the various processes involved in transcellular transport (TT) of huperzine A alone or in combination with ginkgolide B in Caco-2 and Madin-Darby canine renal (MDCK) cell monolayer.
  
  
  METHODS
  The transepithelial passage was assayed in the apical-to-basolateral (AP to BL) direction and opposite direction (BL to AP) in both cell lines. The determination of huperzine A and ginkgolide B were performed by high performance liquid chromatography (HPLC). The passage rates of huperzine A and ginkgolide B were calculated. Bi-directional TT (absorption and secretion) were taken in huperzine A and ginkgolide B in Caco-2 and MDCK cell monolayer.
  
  
  RESULTS
  TT absorption and secretion kinetics of huperzine A and ginkgolide B across two cells existed at the same time. The passage rates of huperzine A were increased significantly with adding different concentrations of ginkgolide B.
  
  
  CONCLUSIONS
  The compound preparations of HA in combination with GB for dementia caused by cerebral ischemic have synergistic effects on the pharmacodynamics, and improve the bioavailability through BBB.
authors.affiliation: Pharmacy Department, 208th Hospital of PLA., Changchun, 130062, Jilin, China.
authors.forename: Wen-Cui
authors.initials: WC
authors.lastname: Zhao
authors.affiliation: Hospital Office, 208th Hospital of PLA., Changchun, 130062, Jilin, China.
authors.forename: Zhi-Hong
authors.initials: ZH
authors.lastname: Hou
authors.affiliation: Pharmacy Department, 208th Hospital of PLA., Changchun, 130062, Jilin, China. Electronic address: zqi8229@126.com.
authors.forename: Qi
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: Infirmary, 208th Hospital of PLA., Changchun, 130062, Jilin, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zheng
keywords: Caco-2; Ginkgolide B; Huperzine A; Madin-Darby canine renal; Transcellular Transport