title: Fabrication of polymeric nanocapsules from curcumin-loaded nanoemulsion templates by self-assembly.
abstract: In this study, biodegradable polymeric nanocapsules were prepared by sequential deposition of food-grade polyelectrolytes through the self-assembling process onto the oil (medium chain triglycerides) droplets enriched with curcumin (lipophilic bioactive compound). Optimum conditions were used to prepare ultrasound-assisted nanoemulsions stabilized by octenyl-succinic-anhydride (OSA)-modified starch. Negatively charged droplets (-39.4 ± 1.84 mV) of these nanoemulsions, having a diameter of 142.7 ± 0.85 nm were used as templates for the fabrication of nanocapsules. Concentrations of layer-forming cationic (chitosan) and anionic (carboxymethylcellulose) biopolymers were optimized based on the mean droplet/particle diameter (MDD/MPD), polydispersity index (PDI) and net charge on the droplets/capsules. Prepared core-shell structures or nanocapsules, having MPD of 159.85 ± 0.92 nm, were characterized by laser diffraction (DLS), ζ-potential (ZP), atomic force microscopy (AFM), transmission electron microscopy (TEM) and confocal laser scanning microscopy (CLSM). Furthermore, physical stability of curcumin-loaded nanocapsules in suspension was determined and compared at different storage temperatures. This study may provide information regarding the formation of ultrasound-assisted polymeric nanocapsules from the nanoemulsion templates which could be helpful in the development of delivery systems for lipophilic food bioactives.
authors.affiliation: State Key Laboratory of Food Science & Technology, School of Food Science & Technology, Jiangnan University, Wuxi 214122, Jiangsu, China.
authors.forename: Shabbar
authors.initials: S
authors.lastname: Abbas
authors.affiliation: State Key Laboratory of Food Science & Technology, School of Food Science & Technology, Jiangnan University, Wuxi 214122, Jiangsu, China.
authors.forename: Eric
authors.initials: E
authors.lastname: Karangwa
authors.affiliation: State Key Laboratory of Food Science & Technology, School of Food Science & Technology, Jiangnan University, Wuxi 214122, Jiangsu, China.
authors.forename: Mohanad
authors.initials: M
authors.lastname: Bashari
authors.affiliation: Department of Chemistry, COMSATS Institute of Information Technology, Abbotabad 22060, Pakistan.
authors.forename: Khizar
authors.initials: K
authors.lastname: Hayat
authors.affiliation: State Key Laboratory of Food Science & Technology, School of Food Science & Technology, Jiangnan University, Wuxi 214122, Jiangsu, China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Hong
authors.affiliation: State Key Laboratory of Food Science & Technology, School of Food Science & Technology, Jiangnan University, Wuxi 214122, Jiangsu, China.
authors.forename: Hafiz Rizwan
authors.initials: HR
authors.lastname: Sharif
authors.affiliation: State Key Laboratory of Food Science & Technology, School of Food Science & Technology, Jiangnan University, Wuxi 214122, Jiangsu, China. Electronic address: xmzhang@jiangnan.edu.cn.
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Zhang
mesh_terms: D000327:Adsorption; D002266:Carboxymethylcellulose Sodium / Q000737:chemistry; D048271:Chitosan / Q000737:chemistry; D003474:Curcumin / Q000737:chemistry*; D004355:Drug Stability; D004655:Emulsions; D057927:Hydrophobic and Hydrophilic Interactions; D008970:Molecular Weight; D053769:Nanocapsules / Q000737:chemistry*; D010316:Particle Size; D011108:Polymers / Q000737:chemistry*; D013213:Starch / Q000031:analogs & derivatives / Q000737:chemistry; D014280:Triglycerides / Q000737:chemistry; D014465:Ultrasonics
chemical_list: D004655:Emulsions; D053769:Nanocapsules; D011108:Polymers; D014280:Triglycerides; C551966:octenyl succinic anhydride-modified starch; D013213:Starch; D048271:Chitosan; D003474:Curcumin; D002266:Carboxymethylcellulose Sodium
keywords: Microscopy; Nanocapsules; Nanoemulsion template; Polyelectrolytes; Sonication