title: Ski diminishes TGF-β1-induced myofibroblast phenotype via up-regulating Meox2 expression.
abstract: OBJECTIVE
  The aim of the present work was to investigate the mechanism of transforming growth factor (TGF)-β1 and Sloan-Kettering Institute (Ski) in the pathogenesis of hypertrophic scars (HS).
  
  
  BACKGROUND
  Wound healing is an inherent process, but the aberrant wound healing of skin injury may lead to HS. There has been growing evidence suggesting a role for TGF-β1 and Ski in the pathogenesis of fibrosis.
  
  
  METHODS
  The MTT assay was used to detect the cell proliferation induced by TGF-β1. The Ski gene was transduced into cells with an adenovirus, and then the function of Ski in cell proliferation and differentiation was observed. Ski mRNA levels were measured by RT-PCR. Western blotting was used to detect the protein expression of α-SMA, E-cadherin, Meox1, Meox2, Zeb1 and Zeb2.
  
  
  RESULTS
  TGF-β1 can promote human skin fibroblast (HSF) cell proliferation in a time-dependent manner, but the promoting effect could be suppressed by Ski. TGF-β1 also induces the formation of the myofibroblast phenotype and the effect of TGF-β1 could be diminished by Ski. Also, Ski modulates the cardiac myofibroblast phenotype and function through suppression of Zeb2 by up-regulating the expression of Meox2.
  
  
  CONCLUSIONS
  Ski diminishes the myofibroblast phenotype induced by TGF-β1 through the suppression of Zeb2 by up-regulating the expression of Meox2.
authors.affiliation: Department of Burns and Plastic Surgery, Liao Cheng Hospital, Liao Cheng 252000, China. Electronic address: zhaowei_chen@163.com.
authors.forename: Zhaowei
authors.initials: Z
authors.lastname: Chen
authors.affiliation: Department of Burns and Plastic Surgery, Liao Cheng Hospital, Liao Cheng 252000, China.
authors.forename: Wenjing
authors.initials: W
authors.lastname: Li
authors.affiliation: Department of Burns and Plastic Surgery, Liao Cheng Hospital, Liao Cheng 252000, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Ning
authors.affiliation: Department of Burns and Plastic Surgery, Liao Cheng Hospital, Liao Cheng 252000, China.
authors.forename: Tong
authors.initials: T
authors.lastname: Liu
authors.affiliation: Department of Burns and Plastic Surgery, Liao Cheng Hospital, Liao Cheng 252000, China.
authors.forename: Jingxiang
authors.initials: J
authors.lastname: Shao
authors.affiliation: Department of Burns and Skin Surgery, Xi Jing Hospital, Xian 710032, China.
authors.forename: Yaojun
authors.initials: Y
authors.lastname: Wang
mesh_terms: D015153:Blotting, Western; D002454:Cell Differentiation / Q000502:physiology; D049109:Cell Proliferation / Q000502:physiology; D002478:Cells, Cultured; D017439:Cicatrix, Hypertrophic / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000378:metabolism*; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism; D005786:Gene Expression Regulation; D018398:Homeodomain Proteins / Q000378:metabolism*; D006801:Humans; D058628:Myofibroblasts / Q000166:cytology / Q000378:metabolism*; D010641:Phenotype; D011518:Proto-Oncogene Proteins / Q000378:metabolism*; D034741:RNA, Small Interfering; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014162:Transfection; D053773:Transforming Growth Factor beta1 / Q000378:metabolism*; D015854:Up-Regulation; D014945:Wound Healing / Q000502:physiology
chemical_list: D004268:DNA-Binding Proteins; D018398:Homeodomain Proteins; C484193:MEOX2 protein, human; D011518:Proto-Oncogene Proteins; D034741:RNA, Small Interfering; D053773:Transforming Growth Factor beta1; C060614:SKI protein, human
keywords: Expression of Meox2; Ski; TGF-β1