title: A method for determining supernatant-free spectra generated from fluorescently stained cells in suspension.
abstract: Here we present a fluorometric method for direct determination of supernatant-free fluorescence spectra generated from fluorescently stained cells in suspension. The key element in the new technique is the design of an adapter to a standard cuvette holder that makes it possible to measure front-face fluorescence spectra from thin layers of cells spun down to the bottom of a spectrofluorometric cuvette. We have demonstrated the applicability of this approach and its analytical potential using the suspensions of yeast cells stained with the potentiometric dye of 3,3'-dipropylthiadicarbocyanine, diS-C3(3), and with the specific cell-wall marker calcofluor.
authors.affiliation: Charles University, Faculty of Mathematics and Physics, Ke Karlovu 3, 12116 Prague, Czech Republic. Electronic address: plasek@karlov.mff.cuni.cz.
authors.forename: Jaromír
authors.initials: J
authors.lastname: Plášek
authors.affiliation: Charles University, Faculty of Mathematics and Physics, Ke Karlovu 3, 12116 Prague, Czech Republic.
authors.forename: Dana
authors.initials: D
authors.lastname: Gášková
mesh_terms: D002232:Carbocyanines / Q000737:chemistry; D008564:Membrane Potentials; D012441:Saccharomyces cerevisiae / Q000166:cytology; D013050:Spectrometry, Fluorescence / Q000295:instrumentation
chemical_list: C091791:3,3'-dipropylthiacarbocyanine; D002232:Carbocyanines