title: Surface assembly of nano-metal organic framework on amine functionalized indium tin oxide substrate for impedimetric sensing of parathion.
abstract: The present paper reports the assembly and pesticide sensing application of a nanometal organic framework [Cd(atc)(H2O)2]n ('atc'=2-aminoterephthalic acid). The assembly of the NMOF film has been achieved by sequential dipping of a 2-aminobenzylamine (2-ABA) modified indium tin oxide (ITO) slide in organic linker 'atc' and metal ion 'Cd(2+)' solutions. The different structural and morphological characteristics of the NMOF thin film have been characterized. The availability of pendent -COOH functional groups on the assembled NMOF film is exploited to synthesize a pesticide immunosensor by conjugating the NMOF film with anti-parathion antibody. This immunosensor has been explored for the electrochemical impedance spectroscopy (EIS) based analysis of parathion in the concentration range of 0.1-20ng/mL. The proposed detection is specific with respect to other organophosphate compounds, e.g. malathion, paraoxon, fenitrothion, monochrotophos and dichlorovos. The proposed sensor shows the detection limit of 0.1ng/mL and it is applicable for analysis of parathion in a rice sample. The sensor's performance is validated by comparting the obtained results with gas chromatographic data.
authors.affiliation: Central Scientific Instruments Organisation (CSIR-CSIO), Sector 30C, Chandigarh 160030, India; Academy of Scientific and Innovative Research, CSIR-CSIO, Sector 30C, Chandigarh 160030, India. Electronic address: dr.akashdeep@csio.res.in.
authors.forename: Akash
authors.initials: A
authors.lastname: Deep
authors.affiliation: Central Scientific Instruments Organisation (CSIR-CSIO), Sector 30C, Chandigarh 160030, India; Academy of Scientific and Innovative Research, CSIR-CSIO, Sector 30C, Chandigarh 160030, India.
authors.forename: Sanjeev Kumar
authors.initials: SK
authors.lastname: Bhardwaj
authors.affiliation: Central Scientific Instruments Organisation (CSIR-CSIO), Sector 30C, Chandigarh 160030, India; Academy of Scientific and Innovative Research, CSIR-CSIO, Sector 30C, Chandigarh 160030, India.
authors.forename: A K
authors.initials: AK
authors.lastname: Paul
authors.affiliation: Department of Civil & Environmental Engineering, Hanyang University, 222 Wangsimni-Ro, Seoul 133-791, Republic of Korea.
authors.forename: Ki-Hyun
authors.initials: KH
authors.lastname: Kim
authors.affiliation: Central Scientific Instruments Organisation (CSIR-CSIO), Sector 30C, Chandigarh 160030, India; Academy of Scientific and Innovative Research, CSIR-CSIO, Sector 30C, Chandigarh 160030, India.
authors.forename: Pawan
authors.initials: P
authors.lastname: Kumar
mesh_terms: D000586:Amination; D055910:Antibodies, Immobilized / Q000737:chemistry; D015374:Biosensing Techniques / Q000379:methods; D002104:Cadmium / Q000737:chemistry; D058266:Dielectric Spectroscopy / Q000379:methods*; D007306:Insecticides / Q000032:analysis*; D057230:Limit of Detection; D049329:Nanostructures / Q000737:chemistry*; D009942:Organometallic Compounds / Q000737:chemistry*; D012275:Oryza / Q000737:chemistry*; D010278:Parathion / Q000032:analysis*; D010795:Phthalic Acids / Q000737:chemistry; D017971:Tin Compounds / Q000737:chemistry*
chemical_list: D055910:Antibodies, Immobilized; D007306:Insecticides; D009942:Organometallic Compounds; D010795:Phthalic Acids; D017971:Tin Compounds; D002104:Cadmium; D010278:Parathion; C109984:indium tin oxide
keywords: Impedance; Metal organic framework; Parathion; Self-assembly; Sensing