title: Protective immunity against Eimeria tenella infection in chickens induced by immunization with a recombinant C-terminal derivative of EtIMP1.
abstract: Immune mapped protein-1 (IMP1) is a new protective protein in apicomplexan parasites, and exits in Eimeria tenella. Cloning and sequence analysis has predicted the antigen to be a novel membrane protein of apicomplexan parasites. In order to assess the immunogenicity of EtIMP1, a C-terminal derivative of EtIMP1 was expressed in a bacterial host system and was used to immunize chickens. The protective efficacy against a homologous challenge was evaluated by body weight gains, lesion scores and fecal oocyst shedding. The results showed that the subunit vaccine can improve weight gains, reduced cecal pathology and lower oocyst fecal shedding compared with non immunized controls. The results suggested that the C-terminal derivative of EtIMP1 might be considered as a candidate in the development of subunit vaccines against Eimeria infection.
authors.affiliation: Engineering Laboratory of Animal Pharmaceuticals, College of Animal Science, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian Province, China.
authors.forename: Guangwen
authors.initials: G
authors.lastname: Yin
authors.affiliation: Engineering Laboratory of Animal Pharmaceuticals, College of Animal Science, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian Province, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Lin
authors.affiliation: Engineering Laboratory of Animal Pharmaceuticals, College of Animal Science, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian Province, China.
authors.forename: Wenjun
authors.initials: W
authors.lastname: Wei
authors.affiliation: National Animal Protozoa Laboratory & College of Veterinary Medicine, China Agricultural University, Beijing 100193, China.
authors.forename: Mei
authors.initials: M
authors.lastname: Qin
authors.affiliation: National Animal Protozoa Laboratory & College of Veterinary Medicine, China Agricultural University, Beijing 100193, China; Key Laboratory of Zoonosis, China Ministry of Agriculture & College of Veterinary Medicine, China Agricultural University, Beijing 100193, China.
authors.forename: Xianyong
authors.initials: X
authors.lastname: Liu
authors.affiliation: National Animal Protozoa Laboratory & College of Veterinary Medicine, China Agricultural University, Beijing 100193, China; Key Laboratory of Zoonosis, China Ministry of Agriculture & College of Veterinary Medicine, China Agricultural University, Beijing 100193, China.
authors.forename: Xun
authors.initials: X
authors.lastname: Suo
authors.affiliation: Engineering Laboratory of Animal Pharmaceuticals, College of Animal Science, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian Province, China. Electronic address: huangzj1999@sina.com.
authors.forename: Zhijian
authors.initials: Z
authors.lastname: Huang
mesh_terms: D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood; D002645:Chickens; D003048:Coccidiosis / Q000276:immunology / Q000469:parasitology / Q000517:prevention & control / Q000662:veterinary*; D016786:Eimeria tenella / Q000235:genetics / Q000276:immunology*; D005243:Feces / Q000469:parasitology; D008297:Male; D008565:Membrane Proteins / Q000235:genetics / Q000276:immunology*; D010270:Parasite Egg Count / Q000662:veterinary; D011201:Poultry Diseases / Q000276:immunology / Q000469:parasitology* / Q000517:prevention & control; D016052:Protozoan Vaccines / Q000276:immunology*; D011897:Random Allocation; D014611:Vaccination / Q000379:methods / Q000662:veterinary; D022223:Vaccines, Subunit / Q000008:administration & dosage / Q000276:immunology* / Q000592:standards
chemical_list: D000913:Antibodies, Protozoan; D008565:Membrane Proteins; D016052:Protozoan Vaccines; D022223:Vaccines, Subunit
keywords: Coccidiosis; Eimeria tenella; Immune mapped protein1; Vaccine