title: A subchronic toxicity study, preceded by an in utero exposure phase, with refined arachidonic acid-rich oil (RAO) derived from Mortierella alpina XM027 in rats.
abstract: To evaluate the potential toxicity of refined arachidonic acid-rich oil (RAO) derived from Mortierella alpina (M. alpina) XM027, we performed a 90-day subchronic study in F1 Sprague Dawley (SD) rats. This study was preceded by a 4-week pretreatment period of parental (F0) rats and exposure of the F0 dams throughout mating, gestation, and lactation. The results indicated that RAO, at dose levels of 0.5%, 1.5%, and 5%, did not affect either reproductive performance of the parental rats, or any characteristics of the pups. In the subchronic study with the offspring (F1) rats, no treatment related abnormalities were observed. In summary, no observable adverse effect level (NOAEL) in this study was placed at 5% RAO, the highest level tested. This level corresponds to approximately 3750mg/kg in F0 females, 2850mg/kg in F0 males, 4850mg/kg in F1 females, and 4480mg/kg in F1 males.
authors.affiliation: School of Life Sciences, Yantai University, Yantai, Shandong 264005, PR China.
authors.forename: Yonglin
authors.initials: Y
authors.lastname: Gao
authors.affiliation: School of Pharmacy, Yantai University, Yantai, Shandong 264005, PR China. Electronic address: gylbill@163.com.
authors.forename: Chunmei
authors.initials: C
authors.lastname: Li
authors.affiliation: School of Life Sciences, Yantai University, Yantai, Shandong 264005, PR China.
authors.forename: Le
authors.initials: L
authors.lastname: Kang
authors.affiliation: School of Life Sciences, Yantai University, Yantai, Shandong 264005, PR China.
authors.forename: Bing
authors.initials: B
authors.lastname: Hang
authors.affiliation: School of Pharmacy, Yantai University, Yantai, Shandong 264005, PR China.
authors.forename: Mengjun
authors.initials: M
authors.lastname: Yan
authors.affiliation: School of Pharmacy, Yantai University, Yantai, Shandong 264005, PR China.
authors.forename: Shenjun
authors.initials: S
authors.lastname: Li
authors.affiliation: School of Life Sciences, Yantai University, Yantai, Shandong 264005, PR China.
authors.forename: Haizhu
authors.initials: H
authors.lastname: Jin
authors.affiliation: NutraSource, Inc., Royal Oak, MI 48073, USA.
authors.forename: Albert W
authors.initials: AW
authors.lastname: Lee
authors.affiliation: NutraSource, Inc., Clarksville, MD 21029, USA.
authors.forename: Susan S
authors.initials: SS
authors.lastname: Cho
mesh_terms: D000818:Animals; D016718:Arachidonic Acid / Q000633:toxicity*; D005260:Female; D007774:Lactation; D008297:Male; D008431:Maternal-Fetal Exchange; D020094:Mortierella; D019055:No-Observed-Adverse-Effect Level; D010938:Plant Oils / Q000633:toxicity*; D011247:Pregnancy; D017207:Rats, Sprague-Dawley; D059550:Toxicity Tests, Subchronic
chemical_list: D010938:Plant Oils; D016718:Arachidonic Acid
keywords: Mortierella alpina (M. alpina) XM027; Refined arachidonic acid-rich oil (RAO); Reproductive performance; Subchronic toxicity