title: Lipophilic antioxidants prevent lipopolysaccharide-induced mitochondrial dysfunction through mitochondrial biogenesis improvement.
abstract: Oxidative stress is implicated in several infectious diseases. In this regard, lipopolysaccharide (LPS), an endotoxic component, induces mitochondrial dysfunction and oxidative stress in several pathological events such as periodontal disease or sepsis. In our experiments, LPS-treated fibroblasts provoked increased oxidative stress, mitochondrial dysfunction, reduced oxygen consumption and mitochondrial biogenesis. After comparing coenzyme Q10 (CoQ10) and N-acetylcysteine (NAC), we observed a more significant protection of CoQ10 than of NAC, which was comparable with other lipophilic and hydrophilic antioxidants such as vitamin E or BHA respectively. CoQ10 improved mitochondrial biogenesis by activating PGC-1α and TFAM. This lipophilic antioxidant protection was observed in mice after LPS injection. These results show that mitochondria-targeted lipophilic antioxidants could be a possible specific therapeutic strategy in pharmacology in the treatment of infectious diseases and their complications.
authors.affiliation: Department of Periodontology, Dental School, University of Sevilla, Spain; Research Laboratory, Dental School, University of Sevilla, Sevilla, Spain.
authors.forename: Pedro
authors.initials: P
authors.lastname: Bullón
authors.affiliation: Research Laboratory, Dental School, University of Sevilla, Sevilla, Spain.
authors.forename: Lourdes
authors.initials: L
authors.lastname: Román-Malo
authors.affiliation: Research Laboratory, Dental School, University of Sevilla, Sevilla, Spain.
authors.forename: Fabiola
authors.initials: F
authors.lastname: Marín-Aguilar
authors.affiliation: Dipartimento di Scienze Cliniche Specialistiche ed Odontostomatologiche (DISCO)-Sez. Biochimica, Facoltà di Medicina, Università Politecnica delle Marche, Ancona 60131, Italy; Area de Nutrición y Salud, Universidad Internacional Iberoamericana (UNINI), Campeche C.P. 24040, Mexico; Facultad de Ciencias de la Salud, Universidad Nacional de Chimborazo, Riobamba, Ecuador.
authors.forename: José Miguel
authors.initials: JM
authors.lastname: Alvarez-Suarez
authors.affiliation: Dipartimento di Scienze Agrarie, Alimentari e Ambientali (D3A), Università Politecnica delle Marche, Via Ranieri 65, Ancona 60131, Italy.
authors.forename: Francesca
authors.initials: F
authors.lastname: Giampieri
authors.affiliation: Dipartimento di Scienze Cliniche Specialistiche ed Odontostomatologiche (DISCO)-Sez. Biochimica, Facoltà di Medicina, Università Politecnica delle Marche, Ancona 60131, Italy; Director Centre for Nutrition & Health, Universidad Europea del Atlantico (UEA), Santander 39011, Spain. Electronic address: m.a.battino@univpm.it.
authors.forename: Maurizio
authors.initials: M
authors.lastname: Battino
authors.affiliation: Research Laboratory, Dental School, University of Sevilla, Sevilla, Spain. Electronic address: mdcormor@us.es.
authors.forename: Mario D
authors.initials: MD
authors.lastname: Cordero
mesh_terms: D000111:Acetylcysteine / Q000494:pharmacology*; D000328:Adult; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002478:Cells, Cultured; D002950:Citrate (si)-Synthase / Q000378:metabolism; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D015227:Lipid Peroxidation / Q000187:drug effects; D008070:Lipopolysaccharides; D008297:Male; D008810:Mice, Inbred C57BL; D008928:Mitochondria / Q000187:drug effects* / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D010101:Oxygen Consumption / Q000187:drug effects; D017382:Reactive Oxygen Species / Q000378:metabolism; D014451:Ubiquinone / Q000031:analogs & derivatives* / Q000494:pharmacology
chemical_list: D000975:Antioxidants; D008070:Lipopolysaccharides; D017382:Reactive Oxygen Species; D014451:Ubiquinone; D002950:Citrate (si)-Synthase; C024989:coenzyme Q10; D000111:Acetylcysteine
keywords: Coenzyme Q(10); Lipopolysaccharide; Mitochondria; N-acetylcysteine; Porphyromonas gingivalis