title: 1-deoxynojirimycin isolated from Bacillus subtilis improves hepatic lipid metabolism and mitochondrial function in high-fat-fed mice.
abstract: The aim of this study was to determine whether 1-deoxynojirimycin (DNJ) isolated from Bacillus subtilis MORI beneficially influences lipid metabolism and mitochondrial function in the liver of mice fed a high-fat diet in addition to the anti-obesity properties of DNJ. Male C57BL/6 mice (n = 29; 5 weeks old) were randomly assigned to three groups: normal control diet (CTL, n = 10), high-fat diet (HF, n = 10), and high-fat diet supplemented with DNJ (DNJ, n = 9). After 12 weeks, the HF group exhibited higher overall weight gain, of the liver, and of various fat pads than the CTL and DNJ groups did. The HF group also showed greater expression of C/EBPα and CD36 mRNA in the liver than that in the CTL and/or DNJ groups. In addition, mRNA expressions of AAC and FAS were lower, while mRNA expression of PGC-1β was higher in the liver of the DNJ group than that of the HF group. The hepatic expression of p-AMPK/AMPK was higher in the DNJ group than in the HF group. This study provides novel insight into the protective effect of DNJ supplementation against obesity-induced hepatic lipid abnormalities and mitochondrial dysfunction.
authors.affiliation: Department of Food and Nutrition, Korea University, Seoul 136-704, Republic of Korea.
authors.forename: Hyun Ju
authors.initials: HJ
authors.lastname: Do
authors.affiliation: Department of Applied Bioscience, CHA University, Gyeonggi-do 463-400, Republic of Korea.
authors.forename: Ji Hyung
authors.initials: JH
authors.lastname: Chung
authors.affiliation: Department of Food and Nutrition, Korea University, Seoul 136-704, Republic of Korea; Department of Public Health Sciences, Graduate School, Korea University, Seoul 136-703, Republic of Korea.
authors.forename: Ji Won
authors.initials: JW
authors.lastname: Hwang
authors.affiliation: Department of Food Science and Nutrition, Dong-A University, Busan, Republic of Korea.
authors.forename: Oh Yoen
authors.initials: OY
authors.lastname: Kim
authors.affiliation: R&D Center for Natural Science, Biotopia Co., Ltd., Chuncheon 200-883, Republic of Korea.
authors.forename: Jae-Yeon
authors.initials: JY
authors.lastname: Lee
authors.affiliation: Department of Food and Nutrition, Korea University, Seoul 136-704, Republic of Korea; Department of Public Health Sciences, Graduate School, Korea University, Seoul 136-703, Republic of Korea; Korea University Guro Hospital, Korea University, Seoul 152-703, Republic of Korea. Electronic address: mjshin@korea.ac.kr.
authors.forename: Min-Jeong
authors.initials: MJ
authors.lastname: Shin
mesh_terms: D017485:1-Deoxynojirimycin / Q000494:pharmacology*; D055372:AMP-Activated Protein Kinases / Q000378:metabolism; D000103:Acetyl-CoA Carboxylase / Q000378:metabolism; D000273:Adipose Tissue / Q000187:drug effects / Q000378:metabolism; D000818:Animals; D019440:Anti-Obesity Agents / Q000494:pharmacology; D001412:Bacillus subtilis / Q000737:chemistry*; D001835:Body Weight / Q000187:drug effects; D022762:CCAAT-Enhancer-Binding Proteins / Q000378:metabolism; D018955:CD36 Antigens / Q000378:metabolism; D059305:Diet, High-Fat / Q000009:adverse effects*; D019587:Dietary Supplements; D002149:Energy Intake; D064429:Fatty Acid Synthases / Q000378:metabolism; D050356:Lipid Metabolism / Q000187:drug effects*; D008099:Liver / Q000187:drug effects*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008930:Mitochondria, Liver / Q000378:metabolism*; D009765:Obesity / Q000188:drug therapy; D009929:Organ Size / Q000187:drug effects; D000071248:Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha; D010766:Phosphorylation; D014157:Transcription Factors / Q000378:metabolism
chemical_list: D019440:Anti-Obesity Agents; D022762:CCAAT-Enhancer-Binding Proteins; D018955:CD36 Antigens; C530718:CEBPA protein, mouse; D000071248:Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha; C485290:Ppargc1a protein, mouse; D014157:Transcription Factors; D017485:1-Deoxynojirimycin; D064429:Fatty Acid Synthases; D055372:AMP-Activated Protein Kinases; D000103:Acetyl-CoA Carboxylase
keywords: AMPK; DNJ; Lipogenesis; Mitochondria; Obesity