title: Chemical synthesis and biophysical applications of membrane proteins.
abstract: Chemical synthesis or semi-synthesis of membrane proteins can provide unique molecular tools, such as site-specific isotope labeling or post-translationally modified membrane proteins to gain insight into their biophysical and functional characteristics. However, during preparation, purification, and ligation of transmembrane peptides, tremendous challenges are encountered owing to their hydrophobic nature. This review focuses on the recent advances in chemical synthesis strategies of membrane proteins. These strategies help to solubilize the hydrophobic transmembrane peptide sequences under standard purification and chemical ligation conditions to improve their handling properties. Biophysical and functional studies of synthetic membrane proteins are reviewed as well.
authors.affiliation: High Magnetic Field Laboratory, Chinese Academy of Sciences, Hefei, 230031, China.|Department of Chemistry, Tsinghua University, Beijing, 100084, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Zuo
authors.affiliation: Department of Chemistry, Tsinghua University, Beijing, 100084, China.
authors.forename: Shan
authors.initials: S
authors.lastname: Tang
authors.affiliation: High Magnetic Field Laboratory, Chinese Academy of Sciences, Hefei, 230031, China.
authors.forename: Ji-Shen
authors.initials: JS
authors.lastname: Zheng
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D003902:Detergents / Q000737:chemistry; D004952:Esters; D006801:Humans; D006860:Hydrogen Bonding; D057927:Hydrophobic and Hydrophilic Interactions; D007553:Isotope Labeling; D008565:Membrane Proteins / Q000138:chemical synthesis*; D008969:Molecular Sequence Data; D010455:Peptides / Q000138:chemical synthesis*; D057786:Peptidomimetics / Q000138:chemical synthesis*; D011499:Protein Processing, Post-Translational; D012995:Solubility; D012997:Solvents / Q000737:chemistry
chemical_list: D003902:Detergents; D004952:Esters; D008565:Membrane Proteins; D010455:Peptides; D057786:Peptidomimetics; D012997:Solvents
keywords: biophysical application; membrane proteins; native chemical ligation; reversible backbone modification; solubilizing tag