title: Role of Ocimum sanctum leaf extract on dietary supplementation in the transgenic Drosophila model of Parkinson's disease.
abstract: OBJECTIVE
  To evaluate the effect of Ocimum sanctum leaf extract on the dietary supplementation in the transgenic Drosophila model of Parkinson's disease.
  
  
  METHODS
  The effect of Ocimum sanctum leaf extract was studied on the transgenic Drosophila model of flies expressing normal human alpha synuclein (h-αs) in the neurons. O. sanctum extract at final concentrations of 0.042 8 × 10(-4), 0.87 × 10(-4), and 1.85 × 10(-4) g·mL(-1) of diet were established and the flies were allowed to feed for 21 days. The climbing assay and lipid peroxidation were taken as parameters for the study.
  
  
  RESULTS
  The supplementation of O. sanctum extract showed a dose-dependent significant delay in the loss of climbing ability and reduction in oxidative stress in the brain of PD model flies.
  
  
  CONCLUSIONS
  The results of the present study showed that the O. sanctum extract is potent in reducing the PD symptoms in transgenic Drosophila model.
authors.affiliation: Drosophila Transgenic Laboratory, Section of Genetics, Department of Zoology, Faculty of Life Sciences, Aligarh Muslim University, Aligarh, Uttar Pradesh, India. Electronic address: yasir_hasansiddique@rediffmail.com.
authors.forename: Yasir Hasan
authors.initials: YH
authors.lastname: Siddique
authors.affiliation: Forest Entomology Division, Forest Research Institute, Dehradun 248006, UK, India.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Faisal
authors.affiliation: Drosophila Transgenic Laboratory, Section of Genetics, Department of Zoology, Faculty of Life Sciences, Aligarh Muslim University, Aligarh, Uttar Pradesh, India.
authors.forename: Falaq
authors.initials: F
authors.lastname: Naz
authors.affiliation: Drosophila Transgenic Laboratory, Section of Genetics, Department of Zoology, Faculty of Life Sciences, Aligarh Muslim University, Aligarh, Uttar Pradesh, India.
authors.forename: Smita
authors.initials: S
authors.lastname: Jyoti
authors.affiliation: Drosophila Transgenic Laboratory, Section of Genetics, Department of Zoology, Faculty of Life Sciences, Aligarh Muslim University, Aligarh, Uttar Pradesh, India.
authors.lastname: Rahul
mesh_terms: D000818:Animals; D001921:Brain / Q000187:drug effects / Q000378:metabolism; D019587:Dietary Supplements / Q000032:analysis; D004195:Disease Models, Animal; D004330:Drosophila / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D006801:Humans; D015227:Lipid Peroxidation / Q000187:drug effects; D031339:Ocimum / Q000737:chemistry*; D018384:Oxidative Stress / Q000187:drug effects; D010300:Parkinson Disease / Q000188:drug therapy* / Q000378:metabolism; D010936:Plant Extracts / Q000008:administration & dosage*; D018515:Plant Leaves / Q000737:chemistry
chemical_list: D010936:Plant Extracts
keywords: Climbing assay; Drosophila model; Lipid peroxidation; Ocimum sanctum; Parkinson's disease