title: The effect of neuropeptide Y on brown-like adipocyte's differentiation and activation.
abstract: Despite its wide distribution in the central nervous system, the presence of Neuropeptide Y (NPY) in peripheral tissues has been detected. White adipose tissue (WAT) is a new site of NPY synthesis and secretion. The development of brown-like adipocytes in WAT is controlled by hypothalamic NPY neurons through interaction with sympathetic nervous system (SNS). However, whether peripheral NPY has a direct effect on induction of the Uncoupling protein1 (UCP1)-positive adipocytes is unknown. We have used adipocytes derived from C3H10T1/2 stem cells as a model of brown-like adipocyte, and investigated the role of NPY in their differentiation and activation. In general, NPY had no effect on brown adipogenesis of C3H10T1/2 stem cell, but suppressed db-cAMP activation of brown-like adipocytes, which was due to blunting brown fat-relevant gene expression and mitochondrial function. NPY showed suppression in a receptor-dependent manner, inhibition of endogenous cAMP production and cAMP-PKA-dependent pathways p38 MAPK and CREB phosphorylation were involved in the downstream mechanisms. A novel role of NPY in the peripheral is presented, which may help decrease energy expenditure in WAT of obese subjects.
authors.affiliation: Department of Endocrinology and Metabolism, Huashan Hospital, Medical College Fudan University, Shanghai 200040, China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Wan
authors.affiliation: Department of Endocrinology and Metabolism, Huashan Hospital, Medical College Fudan University, Shanghai 200040, China.
authors.forename: Ruidan
authors.initials: R
authors.lastname: Xue
authors.affiliation: Department of Endocrinology and Metabolism, Huashan Hospital, Medical College Fudan University, Shanghai 200040, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Endocrinology and Metabolism, Huashan Hospital, Medical College Fudan University, Shanghai 200040, China.
authors.forename: Qiongyue
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: Department of Endocrinology and Metabolism, Huashan Hospital, Medical College Fudan University, Shanghai 200040, China.
authors.forename: Shan
authors.initials: S
authors.lastname: Huang
authors.affiliation: Department of Endocrinology and Metabolism, Huashan Hospital, Medical College Fudan University, Shanghai 200040, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wu
authors.affiliation: Department of Endocrinology and Metabolism, Huashan Hospital, Medical College Fudan University, Shanghai 200040, China.
authors.forename: Hongying
authors.initials: H
authors.lastname: Ye
authors.affiliation: Department of Endocrinology and Metabolism, Huashan Hospital, Medical College Fudan University, Shanghai 200040, China.
authors.forename: Zhaoyun
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Department of Endocrinology and Metabolism, Huashan Hospital, Medical College Fudan University, Shanghai 200040, China. Electronic address: yimingli@fudan.edu.cn.
authors.forename: Yiming
authors.initials: Y
authors.lastname: Li
mesh_terms: D052437:Adipocytes, Brown / Q000187:drug effects / Q000502:physiology*; D000818:Animals; D051017:Apoptosis Regulatory Proteins / Q000235:genetics / Q000378:metabolism; D002454:Cell Differentiation; D002460:Cell Line; D000242:Cyclic AMP / Q000378:metabolism; D017868:Cyclic AMP-Dependent Protein Kinases / Q000378:metabolism; D015870:Gene Expression; D020868:Gene Silencing; D007453:Iodide Peroxidase / Q000235:genetics / Q000378:metabolism; D007473:Ion Channels / Q000235:genetics / Q000378:metabolism; D051379:Mice; D024101:Mitochondrial Proteins / Q000235:genetics / Q000378:metabolism; D009478:Neuropeptide Y / Q000502:physiology*; D000071248:Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha; D015290:Second Messenger Systems; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D000071256:Uncoupling Protein 1; D000097564:Iodothyronine Deiodinase Type II
chemical_list: D051017:Apoptosis Regulatory Proteins; D000242:Cyclic AMP; D017868:Cyclic AMP-Dependent Protein Kinases; D007453:Iodide Peroxidase; D007473:Ion Channels; D024101:Mitochondrial Proteins; D009478:Neuropeptide Y; D000071248:Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha; D014157:Transcription Factors; D000071256:Uncoupling Protein 1; D000097564:Iodothyronine Deiodinase Type II; C112510:Cidea protein, mouse; C485290:Ppargc1a protein, mouse; C000604372:Ucp1 protein, mouse
keywords: Brown adipogenesis; Brown fat-like adipocytes; C3H10T1/2; Neuropeptide Y