title: One-carbon substrate-based biohydrogen production: microbes, mechanism, and productivity.
abstract: Among four basic mechanisms for biological hydrogen (H2) production, dark fermentation has been considered to show the highest hydrogen evolution rate (HER). H2 production from one-carbon (C1) compounds such as formate and carbon monoxide (CO) is promising because formate is an efficient H2 carrier, and the utilization of CO-containing syngas or industrial waste gas may render the industrial biohydrogen production process cost-effective. A variety of microbes with the formate hydrogen lyase (FHL) system have been identified from phylogenetically diverse groups of archaea and bacteria, and numerous efforts have been undertaken to improve the HER for formate through strain optimization and bioprocess development. CO-dependent H2 production has been investigated to enhance the H2 productivity of various carboxydotrophs via an increase in CO gas-liquid mass transfer rates and the construction of genetically modified strains. Hydrogenogenic CO-conversion has been applied to syngas and by-product gas of the steel-mill process, and this low-cost feedstock has shown to be promising in the production of biomass and H2. Here, we focus on recent advances in the isolation of novel phylogenetic groups utilizing formate or CO, the remarkable genetic engineering that enhances H2 productivity, and the practical implementation of H2 production from C1 substrates.
authors.affiliation: Archaea Biology and Ecogenomics Division, Department of Ecogenomics and Systems Biology, University of Vienna, Althanstraße 14, 1090 Wien, Austria.
authors.forename: Simon K-M R
authors.initials: SKR
authors.lastname: Rittmann
authors.affiliation: Korea Institute of Ocean Science and Technology, Ansan, South Korea; Department of Marine Biotechnology, University of Science and Technology, Daejeon, South Korea.
authors.forename: Hyun Sook
authors.initials: HS
authors.lastname: Lee
authors.affiliation: Korea Institute of Ocean Science and Technology, Ansan, South Korea.
authors.forename: Jae Kyu
authors.initials: JK
authors.lastname: Lim
authors.affiliation: Korea Institute of Ocean Science and Technology, Ansan, South Korea; Department of Marine Biotechnology, University of Science and Technology, Daejeon, South Korea.
authors.forename: Tae Wan
authors.initials: TW
authors.lastname: Kim
authors.affiliation: Korea Institute of Ocean Science and Technology, Ansan, South Korea; Department of Marine Biotechnology, University of Science and Technology, Daejeon, South Korea.
authors.forename: Jung-Hyun
authors.initials: JH
authors.lastname: Lee
authors.affiliation: Korea Institute of Ocean Science and Technology, Ansan, South Korea; Department of Marine Biotechnology, University of Science and Technology, Daejeon, South Korea. Electronic address: sgkang@kiost.ac.
authors.forename: Sung Gyun
authors.initials: SG
authors.lastname: Kang
mesh_terms: D001105:Archaea / Q000378:metabolism*; D001419:Bacteria / Q000378:metabolism*; D018533:Biomass; D002244:Carbon / Q000378:metabolism*; D002248:Carbon Monoxide / Q000378:metabolism; D005285:Fermentation; D005561:Formates / Q000378:metabolism; D006859:Hydrogen / Q000378:metabolism*; D010802:Phylogeny
chemical_list: D005561:Formates; C030544:formic acid; D002244:Carbon; D002248:Carbon Monoxide; D006859:Hydrogen
keywords: Carbon monoxide; Formate; Genetic engineering; H(2) production; One-carbon substrate