title: MiRNA profiles in cerebrospinal fluid from patients with central hypersomnias.
abstract: MicroRNAs (miRNAs) are involved in the pathogenesis of many human diseases, including some neurological disorders. Recently, we have reported dysregulated miRNAs in plasma from patients with central hypersomnias including type 1 and type 2 narcolepsy, and idiopathic hypersomnia. This study addressed whether miRNA levels are altered in the cerebrospinal fluid (CSF) of patients with central hypersomnias. We conducted high-throughput analyses of miRNAs in CSF from patients using quantitative real-time polymerase chain reaction panels. We identified 13, 9, and 11 miRNAs with a more than two-fold change in concentration in CSF from patients with type 1 and type 2 narcolepsy and idiopathic hypersomnia, respectively, compared with matched healthy controls. Most miRNAs differed in more than one of the sleep disorders. However, all miRNAs were detected at low levels in CSF and varied between individuals. None of them showed significant differences in concentrations between groups after correcting for multiple testing, and none could be validated in an independent cohort. Nevertheless, approximately 60% of the most abundant miRNAs in the profile reported here have previously been identified in the CSF of healthy individuals, showing consistency with previous miRNA profiles found in CSF. In conclusion, we were not able to demonstrate distinct levels or patterns of miRNAs in CSF from central hypersomnia patients.
authors.affiliation: Molecular Sleep Laboratory, Department of Diagnostics, Glostrup Hospital, Nordre Ringvej 69, 2600 Glostrup, Denmark; Danish Center for Sleep Medicine, University of Copenhagen, Glostrup Hospital, Glostrup, Denmark.
authors.forename: Anja
authors.initials: A
authors.lastname: Holm
authors.affiliation: Diagnostic Unit and Center for Non-Coding RNA in Technology and Health, Glostrup Research Institute, Glostrup Hospital, Nordre Ringvej 69, 2600 Glostrup, Denmark.
authors.forename: Claus Heiner
authors.initials: CH
authors.lastname: Bang-Berthelsen
authors.affiliation: Danish Center for Sleep Medicine, University of Copenhagen, Glostrup Hospital, Glostrup, Denmark; Norwegian Resource Center for ADHD, TS and Narcolepsy, Oslo University Hospital, Ullevål, Norway.
authors.forename: Stine
authors.initials: S
authors.lastname: Knudsen
authors.affiliation: Department of Neurology, Glostrup Hospital, Nordre Ringvej 69, 2600 Glostrup, Denmark.
authors.forename: Signe
authors.initials: S
authors.lastname: Modvig
authors.affiliation: Molecular Sleep Laboratory, Department of Diagnostics, Glostrup Hospital, Nordre Ringvej 69, 2600 Glostrup, Denmark.
authors.forename: Birgitte Rahbek
authors.initials: BR
authors.lastname: Kornum
authors.affiliation: Molecular Sleep Laboratory, Department of Diagnostics, Glostrup Hospital, Nordre Ringvej 69, 2600 Glostrup, Denmark. Electronic address: steen.gammeltoft@gmail.com.
authors.forename: Steen
authors.initials: S
authors.lastname: Gammeltoft
authors.affiliation: Danish Center for Sleep Medicine, University of Copenhagen, Glostrup Hospital, Glostrup, Denmark. Electronic address: poul.joergen.jennum@regionh.dk.
authors.forename: Poul J
authors.initials: PJ
authors.lastname: Jennum
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D005260:Female; D006801:Humans; D020177:Idiopathic Hypersomnia / Q000134:cerebrospinal fluid / Q000235:genetics*; D008297:Male; D035683:MicroRNAs / Q000134:cerebrospinal fluid*; D009290:Narcolepsy / Q000134:cerebrospinal fluid / Q000235:genetics*; D060888:Real-Time Polymerase Chain Reaction; D012307:Risk Factors; D055815:Young Adult
chemical_list: D035683:MicroRNAs
keywords: Central nervous system; Cerebrospinal fluid; Hypersomnia; Hypocretin; Narcolepsy; miRNA