title: Nevomelanocytic atypia detection by in vivo reflectance confocal microscopy.
abstract: OBJECTIVE
  In vivo reflectance confocal microscopy (RCM) is a promising novel technology for non-invasive early diagnostics of cutaneous melanoma. However, the possibility to detect melanocytic atypia in nevi by means of in vivo RCM remains unknown. The aim of the study was to evaluate the significance of in vivo RCM features of melanocytic atypia for the diagnosis of melanocytic nevi, dysplastic nevi and cutaneous melanoma.
  
  
  METHODS
  A total of 138 melanocytic skin lesions comprising 25 melanocytic nevi, 69 dysplastic nevi and 44 melanomas were analyzed by means of dermoscopy, in vivo RCM and routine histopathology. In vivo RCM images were analyzed for the arrangement of keratinocytes in epidermis, pagetoid cells and junctional melanocytic nests and correlated refractivity aspects of nests with histopathology.
  
  
  RESULTS
  Separately and all together taken the in vivo RCM features of melanocytic atypia were significant in differential diagnosis of benign and malignant melanocytic skin lesions, though none of the features was significant in discriminating nevi without cytologic atypia of dysplastic nevi. In vivo RCM feature of dense cell clusters corresponded with melanin containing nevomelanocytes on histopathology though exact correspondence of non-homogeneous and atypical sparse cell clusters remained questionable.
  
  
  CONCLUSIONS
  Nevus with histopathologically confirmed nevomelanocytic atypia (dysplastic nevus) could not be distinguished from nevus without atypia using analyzed in vivo RCM features of melanocytic atypia. More accurate diagnostics by means of in vivo RCM needs further investigation on reflectance of single and nested cutaneous melanocytes in benign and malignant skin lesions.
authors.affiliation: National Cancer Institute, Vilnius, Lithuania. Electronic address: ingrida.vaisnoriene@gmail.com.
authors.forename: Ingrida
authors.initials: I
authors.lastname: Vaišnorienė
authors.affiliation: National Cancer Institute, Vilnius, Lithuania; Biophotonics Group of Laser research Center, Vilnius University, Vilnius, Lithuania.
authors.forename: Ričardas
authors.initials: R
authors.lastname: Rotomskis
authors.affiliation: National Cancer Institute, Vilnius, Lithuania.
authors.forename: Vytautas
authors.initials: V
authors.lastname: Kulvietis
authors.affiliation: Faculty of Mathematics and Informatics, Vilnius University, Vilnius, Lithuania.
authors.forename: Rimantas
authors.initials: R
authors.lastname: Eidukevičius
authors.affiliation: Department of Anatomy, Histology and Anthropology of Faculty of Medicine, Vilnius University, Vilnius, Lithuania.
authors.forename: Violeta
authors.initials: V
authors.lastname: Zalgevičienė
authors.affiliation: National Center of Pathology, Vilnius University Hospital Santariškių Klinikos, Vilnius, Lithuania.
authors.forename: Aida
authors.initials: A
authors.lastname: Laurinavičienė
authors.affiliation: National Cancer Institute, Vilnius, Lithuania.
authors.forename: Jonas
authors.initials: J
authors.lastname: Venius
authors.affiliation: National Cancer Institute, Vilnius, Lithuania.
authors.forename: Janina
authors.initials: J
authors.lastname: Didžiapetrienė
mesh_terms: D003937:Diagnosis, Differential; D005260:Female; D006801:Humans; D008297:Male; D008545:Melanoma / Q000473:pathology*; D018613:Microscopy, Confocal / Q000379:methods; D009508:Nevus, Pigmented / Q000473:pathology*; D012878:Skin Neoplasms / Q000473:pathology*; D000096142:Cutaneous Malignant Melanoma
keywords: Diagnostic accuracy; Dysplastic nevus; Melanocytic atypia; Melanoma; Reflectance confocal microscopy