title: The effect of hibernation on the morphology and histochemistry of the intestine of the greater mouse-eared bat, Myotis myotis.
abstract: Seasonal variations in morphometry and histochemistry of the intestine have been examined in the active and hibernating greater mouse-eared bat, Myotis myotis, using histological and histochemical techniques. The results of morphometric analyses indicated that hibernation affected the villus height, villus width, crypt depth and crypt width of the duodenum, jejunum and ileum. Histochemical analysis showed that goblet cells of the small and large intestine contain acidic and neutral mucosubstances. According to the results obtained with Alcian Blue (pH 5.8)/PAS staining, hyaluronic acid is dominant in the goblet cells of the small and large intestine during both the hibernation and active periods. Chondroitin sulfate and dermatan sulfate, which are sulfated GAGs, were dominant, and very little heparan sulfate, heparin and keratan sulfate were present. Moreover, sulfated glycoproteins were also detected in the goblet cells of the small intestine in the active animals. The present study demonstrates that hibernation altered the examined morphometric and histochemical parameters of the intestine.
authors.affiliation: Department of Elementary Teaching, Faculty of Education, Trakya University, 22030 Edirne, Turkey. Electronic address: epinarpaksuz@trakya.edu.tr.
authors.forename: Emine Pinar
authors.initials: EP
authors.lastname: Paksuz
mesh_terms: D000818:Animals; D002685:Chiroptera / Q000378:metabolism* / Q000502:physiology*; D002809:Chondroitin Sulfates / Q000378:metabolism; D003871:Dermatan Sulfate / Q000378:metabolism; D004386:Duodenum / Q000378:metabolism; D005260:Female; D020397:Goblet Cells / Q000378:metabolism; D006497:Heparan Sulfate / Q000378:metabolism; D006605:Hibernation / Q000502:physiology*; D007082:Ileum / Q000378:metabolism; D007413:Intestinal Mucosa / Q000378:metabolism*; D007583:Jejunum / Q000378:metabolism; D008297:Male
chemical_list: D002809:Chondroitin Sulfates; D003871:Dermatan Sulfate; D006497:Heparan Sulfate
keywords: Bat; Chiroptera; Glycosaminoglycans; Hibernation; Intestine; Myotis myotis