title: Structural features and antitumor activity of a novel polysaccharide from alkaline extract of Phellinus linteus mycelia.
abstract: A novel high molecular weight polysaccharide (PL-N1) was isolated from alkaline extract of the cultured Phellinus linteus mycelia. The weight average molecular weight (Mw) of PL-N1 was estimated at 343,000kDa. PL-N1 comprised arabinose, xylose, glucose, and galactose in the molar ratio of 4.0:6.7:1.3:1.0. The chemical structure of PL-N1 was investigated by FTIR and NMR spectroscopies and methylation analysis. The results showed that the backbone of PL-N1 comprised (1→4)-linked β-D-xylopyranosyl residues, (1→2)-linked α-D-xylopyranosyl residues, (1→4)-linked α-D-glucopyranosyl residues, (1→5)-linked β-D-arabinofuranosyl residues, (1→4)-linked β-D-xylopyranosyl residues which branched at O-2, and (1→4)-linked β-D-galactopyranosyl residues which branched at O-6. The branches consisted of (1→)-linked α-D-arabinofuranosyl residues. Antitumor activity assay in vitro showed that PL-N1 could inhibit the growth of HepG2 cells to a certain extent in a dose-dependent manner. Thus, PL-N1 may be developed as a potential, natural antitumor agent and functional food.
authors.affiliation: School of Food & Biological Engineering, Jiangsu University, Zhenjiang 212013, China.
authors.forename: Juan-Juan
authors.initials: JJ
authors.lastname: Pei
authors.affiliation: School of Food & Biological Engineering, Jiangsu University, Zhenjiang 212013, China; Physical Processing of Agricultural Products Key Lab of Jiangsu Province, Zhenjiang 212013, Jiangsu, China.
authors.forename: Zhen-Bin
authors.initials: ZB
authors.lastname: Wang
authors.affiliation: School of Food & Biological Engineering, Jiangsu University, Zhenjiang 212013, China; Physical Processing of Agricultural Products Key Lab of Jiangsu Province, Zhenjiang 212013, Jiangsu, China.
authors.forename: Hai-Le
authors.initials: HL
authors.lastname: Ma
authors.affiliation: School of Food & Biological Engineering, Jiangsu University, Zhenjiang 212013, China; Physical Processing of Agricultural Products Key Lab of Jiangsu Province, Zhenjiang 212013, Jiangsu, China. Electronic address: jkyan_27@163.com.
authors.forename: Jing-Kun
authors.initials: JK
authors.lastname: Yan
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry* / Q000494:pharmacology*; D045744:Cell Line, Tumor; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008970:Molecular Weight; D000084682:Phellinus; D010936:Plant Extracts; D011134:Polysaccharides / Q000737:chemistry* / Q000494:pharmacology*; D013329:Structure-Activity Relationship
chemical_list: D000970:Antineoplastic Agents; C526145:Phellinus linteus extract; D010936:Plant Extracts; D011134:Polysaccharides
keywords: Antitumor activity; Phellinus linteus; Polysaccharide; Purification; Structure