title: Oxidative stress in multi-port and single-port cholecystectomy.
abstract: BACKGROUND
  This study was designed to analyze and compare plasma levels of 8-isoprostane (8-epiPGF2α), a biomarker of lipid peroxidation, and uric acid (UA), a marker of the antioxidant status, in standard laparoscopic (LC) and laparoendoscopic single-site cholecystectomy (LSSC).
  
  
  METHODS
  Forty patients with noncomplicated cholelithiasis were randomized to undergo either LSSC (n = 20) or LC (n = 20). The patients had body mass index <30, American Society of Anesthesiologists score I or II, and no previous upper gastrointestinal surgery. Blood samples were taken preoperatively and 6 h and 24 h postoperatively. Levels of 8-epiPGF2α were determined using enzyme-linked immunosorbent assay, whereas levels of UA were calculated using automated analyzer.
  
  
  RESULTS
  No significant differences were observed in operative data among the groups. Levels of 8-epiPGF2α were significantly higher in LSSC compared with LC at 6 h (P = 0.003) and 24 h (P < 0.001). 8-epiPGF2α levels showed significant changes over time in LC (LSSC: P = 0.720, LC: P < 0.001). UA levels were significantly higher in LC compared with LSSC, 24 h postoperatively (P = 0.021). No significant changes over time in the UA levels in both groups (LSSC: P = 0.056, LC: P = 0.205).
  
  
  CONCLUSIONS
  LSSC is associated with increased oxidative stress compared with LC. Further studies are needed to confirm these results.
authors.affiliation: Department of Surgery, "G. Hatzikosta" General Hospital of Ioannina, Ioannina, Greece. Electronic address: pappasg8@gmail.com.
authors.forename: George
authors.initials: G
authors.lastname: Pappas-Gogos
authors.affiliation: Chemistry Department, Laboratory of Biochemistry, Ioannina University, Ioannina, Greece.
authors.forename: Constantinos C
authors.initials: CC
authors.lastname: Tellis
authors.affiliation: Department of Statistics, Medical School, Democritus University of Thrace, Alexandroupolis, Greece.
authors.forename: Grigorios
authors.initials: G
authors.lastname: Trypsianis
authors.affiliation: Department of Surgery, "G. Hatzikosta" General Hospital of Ioannina, Ioannina, Greece.
authors.forename: Konstantinos E
authors.initials: KE
authors.lastname: Tsimogiannis
authors.affiliation: Department of Surgery, "G. Hatzikosta" General Hospital of Ioannina, Ioannina, Greece.
authors.forename: Evangelos C
authors.initials: EC
authors.lastname: Tsimoyiannis
authors.affiliation: Department of Surgery, Democritus University of Thrace, Alexandroupolis, Greece.
authors.forename: Constantinos E
authors.initials: CE
authors.lastname: Simopoulos
authors.affiliation: Department of Surgery, Democritus University of Thrace, Alexandroupolis, Greece.
authors.forename: Michael
authors.initials: M
authors.lastname: Pitiakoudis
authors.affiliation: Chemistry Department, Laboratory of Biochemistry, Ioannina University, Ioannina, Greece.
authors.forename: Alexandros D
authors.initials: AD
authors.lastname: Tselepis
mesh_terms: D000328:Adult; D017081:Cholecystectomy, Laparoscopic / Q000379:methods*; D015237:Dinoprost / Q000031:analogs & derivatives / Q000097:blood; D005260:Female; D006801:Humans; D015227:Lipid Peroxidation; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress; D014527:Uric Acid / Q000097:blood
chemical_list: D014527:Uric Acid; C075750:8-epi-prostaglandin F2alpha; D015237:Dinoprost
keywords: 8-Isoprostane; Laparoendoscopic single-site cholecystectomy; Oxidative stress; Standard laparoscopic cholecystectomy; Uric acid