title: Diagnosis of an outbreak of Salmonella typhimurium in chinchillas (Chinchilla lanigera) by pulsed-field gel electrophoresis.
abstract: Adult chinchillas (Chinchilla lanigera) that had suddenly died in a commercial farm located in La Plata City, Buenos Aires Province, Argentina, in July 2012 were macroscopically, histopathologically, and microbiologically examined. Salmonella enterica serovar Typhimurium (S. Typhimurium) was isolated from the liver, spleen, heart, lungs, kidneys and intestines from each of the five animals evaluated. The five strains were susceptible to ampicillin, cephalotin, cefotaxime, nalidixic acid, gentamicin, streptomycin, chloramphenicol, fosfomycin, nitrofurantoin and trimethoprim-sulfamethoxazole, and resistant to tetracycline. Each of the five S. Typhimurium isolates was analyzed by XbaI- pulsed-field gel electrophoresis (PFGE), showing an identical electrophoretic profile with 15 defined bands, which was found to be identical to pattern ARJPXX01.0220 of the PulseNet Argentine National database of Salmonella PFGE patterns. This is the first work describing the postmortem diagnosis of an outbreak of salmonellosis in chinchillas by using molecular methods such as PFGE.
authors.affiliation: Cátedra de Patología de Aves y Pilíferos y Laboratorio de Diagnóstico de Enfermedades de las Aves y los Pilíferos, Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata, La Plata, Buenos Aires, Argentina. Electronic address: danielgornatti@fcv.unlp.edu.ar.
authors.forename: Carlos D
authors.initials: CD
authors.lastname: Gornatti Churria
authors.affiliation: Cátedra de Microbiología y Laboratorio de Diagnóstico e Investigaciones Bacteriológicas, Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata, La Plata, Buenos Aires, Argentina.
authors.forename: Germán B
authors.initials: GB
authors.lastname: Vigo
authors.affiliation: Cátedra de Patología de Aves y Pilíferos y Laboratorio de Diagnóstico de Enfermedades de las Aves y los Pilíferos, Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata, La Plata, Buenos Aires, Argentina.
authors.forename: Javier
authors.initials: J
authors.lastname: Origlia
authors.affiliation: Servicio de Enterobacterias, Departamento de Bacteriología, Instituto Nacional de Enfermedades Infecciosas, ANLIS "Dr. Carlos G. Malbrán", Buenos Aires, Argentina.
authors.forename: Josefina
authors.initials: J
authors.lastname: Campos
authors.affiliation: Servicio de Enterobacterias, Departamento de Bacteriología, Instituto Nacional de Enfermedades Infecciosas, ANLIS "Dr. Carlos G. Malbrán", Buenos Aires, Argentina.
authors.forename: María
authors.initials: M
authors.lastname: Caffer
authors.affiliation: Cátedra de Patología de Aves y Pilíferos y Laboratorio de Diagnóstico de Enfermedades de las Aves y los Pilíferos, Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata, La Plata, Buenos Aires, Argentina.
authors.forename: Miguel
authors.initials: M
authors.lastname: Píscopo
authors.affiliation: Cátedra de Patología de Aves y Pilíferos y Laboratorio de Diagnóstico de Enfermedades de las Aves y los Pilíferos, Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata, La Plata, Buenos Aires, Argentina.
authors.forename: Miguel
authors.initials: M
authors.lastname: Herrero Loyola
authors.affiliation: Cátedra de Patología de Aves y Pilíferos y Laboratorio de Diagnóstico de Enfermedades de las Aves y los Pilíferos, Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata, La Plata, Buenos Aires, Argentina.
authors.forename: Miguel
authors.initials: M
authors.lastname: Petruccelli
authors.affiliation: Servicio de Enterobacterias, Departamento de Bacteriología, Instituto Nacional de Enfermedades Infecciosas, ANLIS "Dr. Carlos G. Malbrán", Buenos Aires, Argentina.
authors.forename: Mariana
authors.initials: M
authors.lastname: Pichel
mesh_terms: D000822:Animal Husbandry; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001118:Argentina / Q000453:epidemiology; D002682:Chinchilla / Q000382:microbiology*; D004269:DNA, Bacterial / Q000032:analysis*; D015252:Deoxyribonucleases, Type II Site-Specific; D004196:Disease Outbreaks / Q000662:veterinary*; D024901:Drug Resistance, Multiple, Bacterial; D016521:Electrophoresis, Gel, Pulsed-Field; D005759:Gastroenteritis / Q000175:diagnosis / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary; D060005:Genotyping Techniques; D008826:Microbial Sensitivity Tests; D012150:Polymorphism, Restriction Fragment Length; D012376:Rodent Diseases / Q000175:diagnosis* / Q000453:epidemiology / Q000382:microbiology; D012481:Salmonella Infections, Animal / Q000175:diagnosis* / Q000453:epidemiology / Q000382:microbiology; D012486:Salmonella typhimurium / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; C025580:endodeoxyribonuclease XBAI; D015252:Deoxyribonucleases, Type II Site-Specific
keywords: Chinchilla lanigera; Chinchillas de granja; Farmed chinchillas; PFGE; Salmonella Typhimurium; Zoonosis