title: Drug resistance characteristics of Mycobacterium tuberculosis isolates to four first-line antituberculous drugs from tuberculosis patients with AIDS in Beijing, China.
abstract: The objective of this study was to investigate the drug resistance characteristics of Mycobacterium tuberculosis isolates to four first-line antituberculous drugs (ATDs) from tuberculosis (TB) patients with AIDS in Beijing, China. All M. tuberculosis strains were isolated from specimens from TB patients with AIDS hospitalised between April 2010 and October 2012. Isolates were cultured by mycobacterial culture methods and were identified by multilocus PCR. Drug sensitivity testing was performed by the proportion method with the following first-line ATDs: isoniazid; rifampicin; streptomycin; and ethambutol. Results were compared with the drug resistance status of M. tuberculosis strains isolated from TB patients without HIV infection in Beijing. Among 41 M. tuberculosis isolates from TB patients with AIDS, the rates of total drug resistance (58.5%), initial drug resistance (46.7%) and acquired drug resistance (90.9%) were significantly higher than in TB patients without HIV infection (34.1%, 24.5% and 48.5%, respectively; P<0.05). In TB patients with AIDS, the rates of acquired drug resistance (90.9%) and acquired multidrug-resistant TB (MDR-TB) (54.5%) were significantly higher than the rates of initial drug resistance (46.7%) and initial MDR-TB (10.0%) (P<0.05). In patients with TB without HIV infection, the rate of acquired drug resistance (48.5%) was significantly higher than the rate of initial drug resistance (24.5%) (P<0.05). M. tuberculosis drug resistance in TB patients with AIDS is significantly more serious than in TB patients without HIV infection. These results showed that more attention should be paid to M. tuberculosis drug resistance in AIDS patients.
authors.affiliation: Beijing Ditan Hospital, Capital Medical University, Beijing 100015, China.
authors.forename: Gui-ju
authors.initials: GJ
authors.lastname: Gao
authors.affiliation: National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, State Key Laboratory for Infectious Disease Prevention and Control, Beijing 102206, China; Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou, Zhejiang 310003, China.
authors.forename: Lulu
authors.initials: L
authors.lastname: Lian
authors.affiliation: Beijing Ditan Hospital, Capital Medical University, Beijing 100015, China; First Hospital of Tsinghua University, Beijing 100016, China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Sun
authors.affiliation: National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, State Key Laboratory for Infectious Disease Prevention and Control, Beijing 102206, China; Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou, Zhejiang 310003, China.
authors.forename: Jianhao
authors.initials: J
authors.lastname: Wei
authors.affiliation: Beijing Ditan Hospital, Capital Medical University, Beijing 100015, China.
authors.forename: Jiang
authors.initials: J
authors.lastname: Xiao
authors.affiliation: National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, State Key Laboratory for Infectious Disease Prevention and Control, Beijing 102206, China; Department of Pathophysiology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu, Sichuan 610041, China.
authors.forename: Xiaoying
authors.initials: X
authors.lastname: Wang
authors.affiliation: Beijing Ditan Hospital, Capital Medical University, Beijing 100015, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Zhang
authors.affiliation: National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, State Key Laboratory for Infectious Disease Prevention and Control, Beijing 102206, China; Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou, Zhejiang 310003, China.
authors.forename: Xiuqin
authors.initials: X
authors.lastname: Zhao
authors.affiliation: Beijing Ditan Hospital, Capital Medical University, Beijing 100015, China.
authors.forename: Di
authors.initials: D
authors.lastname: Yang
authors.affiliation: Beijing Ditan Hospital, Capital Medical University, Beijing 100015, China.
authors.forename: Hong-xin
authors.initials: HX
authors.lastname: Zhao
authors.affiliation: Beijing Ditan Hospital, Capital Medical University, Beijing 100015, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Zhao
authors.affiliation: Beijing Ditan Hospital, Capital Medical University, Beijing 100015, China.
authors.forename: Hui-zhu
authors.initials: HZ
authors.lastname: Wang
authors.affiliation: National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, State Key Laboratory for Infectious Disease Prevention and Control, Beijing 102206, China; Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou, Zhejiang 310003, China. Electronic address: wankanglin@icdc.cn.
authors.forename: Kang-lin
authors.initials: KL
authors.lastname: Wan
authors.affiliation: Beijing Ditan Hospital, Capital Medical University, Beijing 100015, China. Electronic address: ditanlxw@163.com.
authors.forename: Xing-wang
authors.initials: XW
authors.lastname: Li
mesh_terms: D017088:AIDS-Related Opportunistic Infections / Q000150:complications / Q000188:drug therapy*; D000328:Adult; D000995:Antitubercular Agents / Q000494:pharmacology* / Q000627:therapeutic use; D002681:China; D024881:Drug Resistance, Bacterial; D005260:Female; D006801:Humans; D008297:Male; D009169:Mycobacterium tuberculosis / Q000187:drug effects*; D014376:Tuberculosis / Q000150:complications / Q000188:drug therapy*
chemical_list: D000995:Antitubercular Agents
keywords: AIDS; Drug resistance; Infection; TB; Tuberculosis