title: Grouper (Epinephelus coioides) MyD88 and Tollip: intracellular localization and signal transduction function.
abstract: Myeloid differentiation factor 88 (MyD88) and Toll-interacting protein (Tollip) are two important regulatory proteins of the Toll-like receptor (TLR) signaling pathways. In this paper, a Tollip sequence of grouper (Epinephelus coioides) was identified and the signal transduction functions of Tollip and MyD88 were studied. The full length of E. coioides Tollip (EcTollip) cDNA with an open reading frame (ORF) of 1734 nucleotides encoded a putative protein of 274 amino acid residues. The EcTollip protein had conservative domains with mammalian homologous proteins, and high identity (78%-95%) with other vertebrates. MyD88 and Tollip were distributed in the HeLa cytoplasm in a highly condensed form. Over-expression of MyD88 could activate nuclear factor-κB (NF-κB) and its function was dependent on the death domain and ID domain on the N-terminal. Some important functional sites of mammalian MyD88 also affected fish MyD88 signal transduction. Tollip impaired NF-κB signals activated by MyD88, and its activity was dependent on the coupling of ubiquitin to the endoplasmic reticulum degradation (CUE) domain on the C-terminal. These results suggest that MyD88 and Tollip of fish and mammals are conservative on function during evolution.
authors.affiliation: State Key Laboratory of Biocontrol, Guangdong Province Key Laboratory for Aquatic Economic Animals, School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, PR China.
authors.forename: Yan-Wei
authors.initials: YW
authors.lastname: Li
authors.affiliation: State Key Laboratory of Biocontrol, Guangdong Province Key Laboratory for Aquatic Economic Animals, School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, PR China.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Wang
authors.affiliation: College of Animal Science, South China Agricultural University, Guangzhou 510642, Guangdong Province, PR China.
authors.forename: Ze-Quan
authors.initials: ZQ
authors.lastname: Mo
authors.affiliation: State Key Laboratory of Biocontrol, Guangdong Province Key Laboratory for Aquatic Economic Animals, School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, PR China.
authors.forename: Xia
authors.initials: X
authors.lastname: Li
authors.affiliation: School of Bioscience and Biotechnology, South China University of Technology, Guangzhou 510006, Guangdong Province, PR China.
authors.forename: Xiao-Chun
authors.initials: XC
authors.lastname: Luo
authors.affiliation: College of Animal Science, South China Agricultural University, Guangzhou 510642, Guangdong Province, PR China.
authors.forename: Xue-Ming
authors.initials: XM
authors.lastname: Dan
authors.affiliation: State Key Laboratory of Biocontrol, Guangdong Province Key Laboratory for Aquatic Economic Animals, School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, PR China. Electronic address: lianxing@mail.sysu.edu.cn.
authors.forename: An-Xing
authors.initials: AX
authors.lastname: Li
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D016000:Cluster Analysis; D003593:Cytoplasm / Q000378:metabolism; D017931:DNA Primers / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D057809:HEK293 Cells; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics / Q000378:metabolism*; D008156:Luciferases; D008969:Molecular Sequence Data; D053594:Myeloid Differentiation Factor 88 / Q000235:genetics / Q000378:metabolism*; D016328:NF-kappa B / Q000378:metabolism; D016366:Open Reading Frames; D010473:Perciformes / Q000378:metabolism / Q000502:physiology*; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D015398:Signal Transduction / Q000502:physiology*
chemical_list: D017931:DNA Primers; D018076:DNA, Complementary; D047908:Intracellular Signaling Peptides and Proteins; D053594:Myeloid Differentiation Factor 88; D016328:NF-kappa B; D008156:Luciferases
keywords: Epinephelus coioides; Intracellular localization; Luciferase reporter assay; MyD88; Tollip