title: Optimized triazine-mediated amidation for efficient and controlled functionalization of hyaluronic acid.
abstract: Triazine-based coupling agents have the potential to replace carbodiimides in the functionalization of hyaluronic acid (HA) giving derivatives with high degrees of substitution (DS) under mild conditions with excellent efficiency. Kinetics of the triazine-mediated amidation of HA in aqueous solution were investigated to understand the reaction mechanism and the role of the amine reagent. The DS decreased with increasing basicity of the amine. The water soluble coupling agent was stable under the reaction conditions (t1/2=10 days) in the absence of amines. The activation of HA proceeded quantitatively. The stoichiometry of amine was the limiting factor in the substitution. Functional HA derivatives with DS up to 55% were obtained by the triazine-mediated amidation. They were used successfully to prepare well-defined HA conjugates via the maleimide-thiol and the azide-alkyne "click" reactions.
authors.affiliation: Laboratory of Polymer Chemistry, Department of Chemistry, P.O. Box 55, 00014 University of Helsinki, Finland. Electronic address: tina.borke@helsinki.fi.
authors.forename: Tina
authors.initials: T
authors.lastname: Borke
authors.affiliation: Laboratory of Polymer Chemistry, Department of Chemistry, P.O. Box 55, 00014 University of Helsinki, Finland; Faculty of Pharmacy and Department of Chemistry, Universite de Montreal, CP 6128 Succursale Centre Ville, Montreal, QC H3C 3J7, Canada; World Premier International (WPI) Research Center Initiative, International Center for Materials Nanoarchitectonics (MANA), National Institute for Materials Science (NIMS), 1-1 Namiki, Tsukuba, Ibaraki 305-0044, Japan. Electronic address: francoise.winnik@umontreal.ca.
authors.forename: Françoise M
authors.initials: FM
authors.lastname: Winnik
authors.affiliation: Laboratory of Polymer Chemistry, Department of Chemistry, P.O. Box 55, 00014 University of Helsinki, Finland. Electronic address: heikki.tenhu@helsinki.fi.
authors.forename: Heikki
authors.initials: H
authors.lastname: Tenhu
authors.affiliation: Laboratory of Polymer Chemistry, Department of Chemistry, P.O. Box 55, 00014 University of Helsinki, Finland. Electronic address: sami.hietala@helsinki.fi.
authors.forename: Sami
authors.initials: S
authors.lastname: Hietala
keywords: Azide-alkyne; Carbodiimide; Click reaction; Hyaluronic acid; Maleimide-thiol; Triazine