title: Anti-photoaging effect of aaptamine in UVB-irradiated human dermal fibroblasts and epidermal keratinocytes.
abstract: Chronic exposure to ultraviolet (UV) irradiation causes sunburn, inflammatory responses, skin cancer, and photoaging. Photoaging, in particular, generates reactive oxygen species (ROS) that stimulate mitogen-activated protein kinase (MAPK) signaling and transcription factors. UV irradiation also activates matrix metalloproteinases (MMPs) expression and inactivates collagen synthesis. Aaptamine, a marine alkaloid isolated from the marine sponge, has been reported to have antitumor, antimicrobial, antiviral, and antioxidant activities. However, the photo-protective effects of aaptamine have not been elucidated. In this study, our data demonstrated that aaptamine deactivated UVB-induced MAPK and activator protein-1 signaling by suppressing ROS, resulting in attenuating the expression of MMPs in UVB-irradiated human dermal fibroblasts. Aaptamine also decreased proinflammatory cytokines such as cyclooxygenase-2, tumor necrosis factor-α, interleukin-1β, and nuclear factor-kappa B subunits in UVB-irradiated human keratinocytes. In conclusion, we suggest that aaptamine represents a novel and effective strategy for treatment and prevention of photoaging.
authors.affiliation: a Department of Biomaterials Science and Engineering , Yonsei University , Seoul 120-749 , South Korea.
authors.forename: Min-Ji
authors.initials: MJ
authors.lastname: Kim
authors.forename: Seon Wook
authors.initials: SW
authors.lastname: Woo
authors.forename: Myung-Suk
authors.initials: MS
authors.lastname: Kim
authors.forename: Ji-Eun
authors.initials: JE
authors.lastname: Park
authors.forename: Jae-Kwan
authors.initials: JK
authors.lastname: Hwang
mesh_terms: D004817:Epidermis / Q000378:metabolism; D005347:Fibroblasts / Q000187:drug effects; D006801:Humans; D053583:Interleukin-1beta / Q000378:metabolism; D015603:Keratinocytes / Q000187:drug effects; D020781:Matrix Metalloproteinase 1 / Q000187:drug effects*; D020778:Matrix Metalloproteinase 2 / Q000187:drug effects*; D015394:Molecular Structure; D016328:NF-kappa B / Q000378:metabolism; D009287:Naphthyridines / Q000737:chemistry / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000032:analysis / Q000378:metabolism; D015595:Skin Aging / Q000187:drug effects; D012871:Skin Diseases / Q000517:prevention & control*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D014466:Ultraviolet Rays / Q000009:adverse effects*
chemical_list: D053583:Interleukin-1beta; D016328:NF-kappa B; D009287:Naphthyridines; D017382:Reactive Oxygen Species; D014409:Tumor Necrosis Factor-alpha; D020778:Matrix Metalloproteinase 2; D020781:Matrix Metalloproteinase 1; C043986:aaptamine
keywords: MAPK/AP-1; aaptamine; matrix metalloproteinase; photoaging; reactive oxygen species