title: [Nucleotide sequence analysis for a new HLA-B allele HLA-B*13:01:06*].
abstract: OBJECTIVE
  To confirm a new allele HLA-B*13:01:06 and analyze its nucleotide sequence.
  
  
  METHODS
  Genomic DNA was extracted using a Qiagen DNA extraction kit. Nucleotide sequences of HLA-A, HLA-B, HLA-C and HLA-DRB1 were analyzed by polymerase chain reaction-sequence based typing (PCR-SBT). HLA high-resolution results were assigned, and the nucleotide sequences of HLA-B locus was compared with that of HLA-B*13:01:01.
  
  
  RESULTS
  The nucleotide sequence of the new allele shows a strong similarity to that of HLA-B*13:01:01. One nucleotide in exon 2 has changed from G to A at position 219 (codon 49 GCG>GCA), which however did not result in amino acid change.
  
  
  CONCLUSIONS
  The novel allele verified by sequencing has been submitted to GenBank and officially named as HLA-B*13:01:06 by the World Health Organization HLA Nomenclature Committee.
authors.affiliation: Department of Surgery, Guangzhou First Municipal People's Hospital Affiliated to Guangzhou Medical University, Guangzhou, Guangdong 510180, P. R. China. huang.yingfeng@qq.com.
authors.forename: Baiyu
authors.initials: B
authors.lastname: Xiong
authors.forename: Yin
authors.initials: Y
authors.lastname: Tan
authors.forename: Yingfeng
authors.initials: Y
authors.lastname: Huang
authors.forename: Shaoyu
authors.initials: S
authors.lastname: Yang
authors.forename: Hongtu
authors.initials: H
authors.lastname: Luo
authors.forename: Liang
authors.initials: L
authors.lastname: Shen
authors.forename: Taichen
authors.initials: T
authors.lastname: Zhou
authors.forename: Cangjun
authors.initials: C
authors.lastname: Xiao
mesh_terms: D000483:Alleles; D000595:Amino Acid Sequence; D001483:Base Sequence; D005091:Exons; D015235:HLA-B Antigens / Q000235:genetics*; D006801:Humans; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA
chemical_list: D015235:HLA-B Antigens