title: Facile enzymatic synthesis of sugar 1-phosphates as substrates for phosphorylases using anomeric kinases.
abstract: Three sugar 1-phosphates that are donor substrates for phosphorylases were produced at the gram scale from phosphoenolpyruvic acid and the corresponding sugars by the combined action of pyruvate kinase and the corresponding anomeric kinases in good yields. These sugar 1-phosphates were purified through two electrodialysis steps. α-D-Galactose 1-phosphate was finally isolated as crystals of dipotassium salts. α-D-Mannose 1-phosphate and 2-acetamido-2-deoxy-α-D-glucose 1-phosphate were isolated as crystals of bis(cyclohexylammonium) salts.
authors.affiliation: National Food Research Institute, National Agriculture and Food Research Organization, 2-1-12 Kannondai, Tsukuba, Ibaraki 305-8642, Japan.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Liu
authors.affiliation: National Food Research Institute, National Agriculture and Food Research Organization, 2-1-12 Kannondai, Tsukuba, Ibaraki 305-8642, Japan.
authors.forename: Mamoru
authors.initials: M
authors.lastname: Nishimoto
authors.affiliation: National Food Research Institute, National Agriculture and Food Research Organization, 2-1-12 Kannondai, Tsukuba, Ibaraki 305-8642, Japan. Electronic address: mkitaoka@affrc.go.jp.
authors.forename: Motomitsu
authors.initials: M
authors.lastname: Kitaoka
mesh_terms: D000818:Animals; D060326:Chemistry Techniques, Synthetic; D006005:Phosphorylases / Q000378:metabolism*; D011770:Pyruvate Kinase / Q000378:metabolism*; D011817:Rabbits; D013237:Stereoisomerism; D013403:Sugar Phosphates / Q000138:chemical synthesis* / Q000737:chemistry* / Q000378:metabolism
chemical_list: D013403:Sugar Phosphates; D006005:Phosphorylases; D011770:Pyruvate Kinase
keywords: Anomeric kinase; Electrodialysis; Pyruvate kinase; Substrate for phosphorylase; Sugar 1-phosphate