title: Suppression of TRPM7 inhibits proliferation, migration, and invasion of malignant human glioma cells.
abstract: BACKGROUND
  Glioblastoma multiforme (GBM) is the most common and aggressive primary brain tumor with a dismal prognosis. Despite intensive study on tumor biology, the underlying mechanisms of the unlimited proliferation and progressive local invasion are still poorly understood, and no effective treatment has been developed for GBM patients.
  
  
  OBJECTIVE
  We determine the role of TRPM7 channels in the growth, migration, and infiltration of malignant glioma cells.
  
  
  METHODS
  Using a combination of RT-PCR, Western blot, and patch-clamp techniques, we demonstrated the expression of functional TRPM7 channels of A172 cells, a human glioma cell line, as well as in human glioma tissues. Furthermore, we evaluated the role of TRPM7 in growth, migration, and infiltration of A172 cells with MTT and transwell migration and invasion assays.
  
  
  RESULTS
  We showed the expression of functional TRPM7 channels in both A172 cells and human glioma tissues. Suppression of TRPM7 expression with TRPM7-siRNA dramatically reduced the proliferation, migration, and invasion of A172 cells. Pharmacological inhibition of TRPM7 channel with 2-aminoethoxydiphenyl borate (2-APB) showed a similar effect as TRPM7-siRNA.
  
  
  CONCLUSIONS
  We demonstrate that human glioma cells express functional TRPM7 channel and that activation of this channel plays an important role in the proliferation, migration, and invasion of malignant glioma cells. TRPM7 channel may represent a novel and promising target for therapeutic intervention of malignant glioma.
authors.affiliation: Neuroscience Institute, Morehouse School of Medicine, Atlanta, GA, USA.
authors.forename: Tian-Dong
authors.initials: TD
authors.lastname: Leng
authors.forename: Ming-Hua
authors.initials: MH
authors.lastname: Li
authors.forename: Jian-Feng
authors.initials: JF
authors.lastname: Shen
authors.forename: Ming-Li
authors.initials: ML
authors.lastname: Liu
authors.forename: Xin-Bo
authors.initials: XB
authors.lastname: Li
authors.forename: Hua-Wei
authors.initials: HW
authors.lastname: Sun
authors.forename: Debbie
authors.initials: D
authors.lastname: Branigan
authors.forename: Zhao
authors.initials: Z
authors.lastname: Zeng
authors.forename: Hong-Fang
authors.initials: HF
authors.lastname: Si
authors.forename: Jun
authors.initials: J
authors.lastname: Li
authors.forename: Jeff
authors.initials: J
authors.lastname: Chen
authors.forename: Zhi-Gang
authors.initials: ZG
authors.lastname: Xiong
mesh_terms: D015153:Blotting, Western; D001896:Boron Compounds / Q000494:pharmacology; D001932:Brain Neoplasms / Q000188:drug therapy / Q000503:physiopathology*; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects* / Q000502:physiology; D049109:Cell Proliferation / Q000187:drug effects* / Q000502:physiology; D002478:Cells, Cultured; D005909:Glioblastoma / Q000188:drug therapy / Q000503:physiopathology*; D006801:Humans; D009361:Neoplasm Invasiveness / Q000503:physiopathology*; D018408:Patch-Clamp Techniques; D016133:Polymerase Chain Reaction; D017346:Protein Serine-Threonine Kinases / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D034741:RNA, Small Interfering; D050053:TRPM Cation Channels / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism
chemical_list: D001896:Boron Compounds; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D050053:TRPM Cation Channels; C109986:2-aminoethoxydiphenyl borate; D017346:Protein Serine-Threonine Kinases; C486583:TRPM7 protein, human
keywords: Glioma; Invasion; Migration; Proliferation; TRPM7