title: Determination of lymphocyte subset reference ranges in peripheral blood of healthy adults by a dual-platform flow cytometry method.
abstract: Flow cytometry has emerged as a useful screening approach to evaluate whether specific cell populations are present or absent. Previous studies have shown different reference ranges in several countries. The aim of this study was to determine reference ranges of lymphocyte subsets in peripheral blood by flow cytometric method in Brazilian adults. In this study, relative and absolute reference ranges of lymphocyte subsets were: CD3+: 51.3-83.5%, 718-2494cells/μl; CD4+: 24.4-54.2%, 456-1492cells/μl; CD8+: 12.8-40.2%, 272-1144cells/μl; CD4+CD8+: double-positive 0.01-3.6%, 2-88cells/μl; TCR γδ: 1.0-15.9%, 19-345cells/μl; CD3+CD4-CD8-: 1.2-13.3%, 28-292cells/μl; TCR αβ+: 44.3-77.0%, 855-2384cells/μl; CD4/CD8 ratio: 0.68-3.61; CD19+: 6.3-20.8%, 112-622cells/μl; mature NK cells: 3.1-27.4%, 70-745cells/μl; immature NK cells: 0.08-1.1%, 1-23cells/μl; total NK cells: 3.7-28.5%, 82-760cells/μl; and NKT cells: 0.9-21.4%, 18-488cells/μl. Comparison with other studies showed differences among some of them. This suggests that there are differences among lymphocyte subsets in the worldwide population and also it is important to determine reference ranges in different populations in order to better assess and monitor patients.
authors.affiliation: Postgraduate Program in Pharmacy, Federal University of Santa Catarina, Florianópolis, Brazil.
authors.forename: Renata Cristina Messores
authors.initials: RC
authors.lastname: Rudolf-Oliveira
authors.affiliation: Department of Clinical Analysis, Federal University of Santa Catarina, Florianópolis, Brazil.
authors.forename: Kauê Taneli
authors.initials: KT
authors.lastname: Gonçalves
authors.affiliation: Department of Clinical Analysis, Federal University of Santa Catarina, Florianópolis, Brazil.
authors.forename: Mariana Lenhani
authors.initials: ML
authors.lastname: Martignago
authors.affiliation: Integrated Multidisciplinary Residency Program in Health, Federal University of Santa Catarina, Florianópolis, Brazil.
authors.forename: Vanessa
authors.initials: V
authors.lastname: Mengatto
authors.affiliation: Postgraduate Program in Pharmacy, Federal University of Santa Catarina, Florianópolis, Brazil.
authors.forename: Pâmela Cristina
authors.initials: PC
authors.lastname: Gaspar
authors.affiliation: Postgraduate Program in Pharmacy, Federal University of Santa Catarina, Florianópolis, Brazil.
authors.forename: Ana Carolina Rabello
authors.initials: AC
authors.lastname: de Moraes
authors.affiliation: Master's Program in Health Sciences, University of Southern Santa Catarina, Tubarão, Brazil; Department of Clinical Medicine, Federal University of Santa Catarina, Florianópolis, Brazil.
authors.forename: Rosemeri Maurici
authors.initials: RM
authors.lastname: da Silva
authors.affiliation: Postgraduate Program in Pharmacy, Federal University of Santa Catarina, Florianópolis, Brazil; Department of Clinical Analysis, Federal University of Santa Catarina, Florianópolis, Brazil; Integrated Multidisciplinary Residency Program in Health, Federal University of Santa Catarina, Florianópolis, Brazil.
authors.forename: Maria Luiza
authors.initials: ML
authors.lastname: Bazzo
authors.affiliation: Postgraduate Program in Pharmacy, Federal University of Santa Catarina, Florianópolis, Brazil; Department of Clinical Analysis, Federal University of Santa Catarina, Florianópolis, Brazil; Integrated Multidisciplinary Residency Program in Health, Federal University of Santa Catarina, Florianópolis, Brazil. Electronic address: maria.claudia.silva@ufsc.br.
authors.forename: Maria Cláudia
authors.initials: MC
authors.lastname: Santos-Silva
mesh_terms: D000328:Adult; D015703:Antigens, CD / Q000097:blood* / Q000276:immunology*; D001938:Brazil; D016516:CD4-CD8 Ratio; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D008214:Lymphocytes / Q000166:cytology* / Q000276:immunology* / Q000378:metabolism*; D008297:Male
chemical_list: D015703:Antigens, CD
keywords: Flow cytometry; Lymphocytes; Reference range