title: Assembly of graphene and nickel nanoparticles on anion exchange resin microspheres for the amperometric detection of carbohydrates in combination with capillary electrophoresis.
abstract: Graphene and nickel nanoparticles (NiNPs) were assembled on anion exchange resin (AER) microspheres based on the electrostatic interaction between graphene oxide sheets and AER and the subsequent chemical reduction. The prepared AER@graphene-NiNP composite core-shell microspheres were characterized by scanning electron microscopy, energy dispersive spectroscopy, X-ray diffraction, and Fourier transform infrared spectroscopy. Moreover, they were embedded in the bores of pipette tips to fabricate electrodes. The performance of the novel electrodes was demonstrated by measuring sucrose, glucose and fructose in combination with capillary electrophoresis. The three analytes were well separated within 8min in a 40cm long capillary at a separation voltage of 12kV. The graphene-NiNP composite microsphere electrodes exhibited higher sensitivity (213.578-317.064nAmM(-1)), lower detection limits (0.75-1.05μM) and enhanced separation efficiency in the detection of these carbohydrates. The advantages of the electrodes include ease of fabrication, low cost and pronounced electrocatalytic activity toward carbohydrates, indicating great promise for a wide range of applications.
authors.affiliation: School of Pharmacy, Center for Analysis and Measurement, 826 Zhangheng Road, Shanghai 201203, China.
authors.forename: Ren
authors.initials: R
authors.lastname: Zhang
authors.affiliation: School of Pharmacy, Center for Analysis and Measurement, 826 Zhangheng Road, Shanghai 201203, China.
authors.forename: Sijie
authors.initials: S
authors.lastname: Lu
authors.affiliation: School of Pharmacy, Center for Analysis and Measurement, 826 Zhangheng Road, Shanghai 201203, China.
authors.forename: Luyan
authors.initials: L
authors.lastname: Zhang
authors.affiliation: School of Pharmacy, Center for Analysis and Measurement, 826 Zhangheng Road, Shanghai 201203, China. Electronic address: gangchen@fudan.edu.cn.
authors.forename: Gang
authors.initials: G
authors.lastname: Chen
mesh_terms: D000837:Anion Exchange Resins / Q000737:chemistry*; D002241:Carbohydrates / Q000032:analysis*; D004566:Electrodes; D019075:Electrophoresis, Capillary / Q000379:methods*; D006108:Graphite / Q000737:chemistry*; D057230:Limit of Detection; D058185:Magnetite Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D008855:Microscopy, Electron, Scanning; D008863:Microspheres; D009532:Nickel / Q000737:chemistry*; D010087:Oxides / Q000737:chemistry
chemical_list: D000837:Anion Exchange Resins; D002241:Carbohydrates; D058185:Magnetite Nanoparticles; D010087:Oxides; D006108:Graphite; D009532:Nickel
keywords: Anion exchange resin; Carbohydrate; Electrochemical sensing; Graphene; Microsphere electrode; Nickel nanoparticle