title: Immunomodulatory activity of heparan sulfate mimetics from Escherichia coli K5 capsular polysaccharide in vitro.
abstract: Escherichia coli K5 capsular polysaccharide (K5PS) has been used as starting material to synthesize heparan sulfate (HS) mimetics with various biological properties. This study determined the immunomodulatory activities of K5PS through the sulfation process. The immunomodulatory effects of sulfated K5 polysaccharide derivatives were evaluated in vitro on murine macrophages and lymphocytes. Results indicated that HS mimetics with high sulfation in the O-position stimulated cell proliferation of macrophages and lymphocytes, significantly enhanced cytokine secretion and upregulated the cytotoxicity of NK cells. This study suggests that high sulfation in O-position of HS is required for the immunomodulatory activities.
authors.affiliation: Wuxi Medical School, Jiangnan University, Wuxi 214122, PR China; The Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, PR China.
authors.forename: Liping
authors.initials: L
authors.lastname: Teng
authors.affiliation: School of Pharmaceutical Science, Jiangnan University, Wuxi 214122, PR China.
authors.forename: Haitian
authors.initials: H
authors.lastname: Fu
authors.affiliation: School of Pharmaceutical Science, Jiangnan University, Wuxi 214122, PR China.
authors.forename: Min
authors.initials: M
authors.lastname: Wang
authors.affiliation: Wuxi Medical School, Jiangnan University, Wuxi 214122, PR China.
authors.forename: Chao
authors.initials: C
authors.lastname: Deng
authors.affiliation: Changshan Biochem Pharm (Jiangsu) Co., Ltd., Changzhou 213149, PR China.
authors.forename: Zhixin
authors.initials: Z
authors.lastname: Song
authors.affiliation: The Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, PR China; School of Pharmaceutical Science, Jiangnan University, Wuxi 214122, PR China. Electronic address: jhchenwhut@126.com.
authors.forename: Jinghua
authors.initials: J
authors.lastname: Chen
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000187:drug effects / Q000276:immunology; D016667:Bacterial Capsules / Q000737:chemistry*; D002460:Cell Line; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D016207:Cytokines / Q000276:immunology; D004926:Escherichia coli; D006497:Heparan Sulfate; D007074:Immunoglobulin G / Q000276:immunology; D007155:Immunologic Factors / Q000737:chemistry / Q000494:pharmacology*; D007694:Killer Cells, Natural / Q000187:drug effects / Q000276:immunology; D008264:Macrophages / Q000187:drug effects / Q000276:immunology; D008297:Male; D008813:Mice, Inbred ICR; D013154:Spleen / Q000166:cytology; D013329:Structure-Activity Relationship; D013431:Sulfates / Q000737:chemistry / Q000494:pharmacology*; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology
chemical_list: D016207:Cytokines; D006497:Heparan Sulfate; D007074:Immunoglobulin G; D007155:Immunologic Factors; D013431:Sulfates; C050673:capsular polysaccharide K5
keywords: Escherichia coli; Immunomodulatory activity; K5 capsular polysaccharide; Structure–activity correlation; Sulfation