title: The short- and long-term effects of environmental conditions on anaerobic methane oxidation coupled to nitrite reduction.
abstract: Anaerobic oxidation of methane coupled to nitrite reduction (n-damo) plays an important role in global carbon and nitrogen cycles and also is a potential bioprocess in wastewater treatment. In this work, the effects of environmental conditions – temperature, pH and salinity – on the metabolic activity and growth rate of n-damo bacteria were investigated by short-term batch test and long-term bacterial incubation. Quantitative PCR and 16S rRNA and pmoA gene sequencing were applied to detect the microbial community in the long-term incubation. The results indicated that all the three environmental factors significantly affected the metabolic activity and growth rate of n-damo bacteria and the optimum temperature, pH and salinity were 35 °C, 7.6 and 0 g NaCl L⁻¹, respectively. Notably, salinity adaption of n-damo bacteria was first observed under salinity stress of 20 g NaCl L⁻¹. It's predicted that n-damo process might occur in saline environments and future work could focus on this.
authors.affiliation: Department of Environmental Engineering, Zhejiang University, Hangzhou 310058, China.
authors.forename: Zhanfei
authors.initials: Z
authors.lastname: He
authors.forename: Sha
authors.initials: S
authors.lastname: Geng
authors.forename: Lidong
authors.initials: L
authors.lastname: Shen
authors.forename: Liping
authors.initials: L
authors.lastname: Lou
authors.forename: Ping
authors.initials: P
authors.lastname: Zheng
authors.forename: Xinhua
authors.initials: X
authors.lastname: Xu
authors.forename: Baolan
authors.initials: B
authors.lastname: Hu
mesh_terms: D000222:Adaptation, Physiological / Q000187:drug effects; D000693:Anaerobiosis; D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D004783:Environmental Microbiology; D006863:Hydrogen-Ion Concentration; D008697:Methane / Q000378:metabolism*; D009573:Nitrites / Q000378:metabolism*; D010084:Oxidation-Reduction; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D054712:Salinity; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride / Q000494:pharmacology; D013312:Stress, Physiological / Q000187:drug effects; D013696:Temperature; D013997:Time Factors
chemical_list: D001426:Bacterial Proteins; D009573:Nitrites; D012336:RNA, Ribosomal, 16S; D012965:Sodium Chloride; D008697:Methane