title: Human placenta-derived Wnt-5a induces the expression of ICAM-1 and VCAM-1 in CD133(+)CD34(+)-hematopoietic progenitor cells.
abstract: Angiogenesis and vascular development are essential for fetal development and growth, whereby early pregnancy loss and other pregnancy-related pathologies have been linked to aberrant vascular development. As Wnt signalling has been suggested to play a role in the vascularization of chorionic villi, we investigated the expression of Wnt family members in trophoblasts and stromal cells isolated from chorionic villi of early placenta and the influence of Wnt signalling on CD133(+)CD34(+)-hematopoietic progenitor (CD133(+)CD34(+)) cells. Wnt-5a was expressed in human placental stromal cells and to a lesser extent in human trophoblast cells. rWnt-5a impeded migration and induced adhesion of CD133(+)CD34(+) cells, in accordance with the expression of adhesion proteins, intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1). rWnt-5a-related regulation of the ICAM-1 and VCAM-1 expression were dependent on the release of Ca2+ and the activation of transcription factor - nuclear factor of activated T-cells (NF-AT). We propose that Wnt-5a is required during early placenta development to mediate adhesion and homing of CD133(+)CD34(+) cells.
authors.affiliation: Department of Obstetrics and Gynecology, Ernst-Moritz-Arndt-University, D-17475 Greifswald, Germany.
authors.forename: Friederike
authors.initials: F
authors.lastname: Herr
authors.affiliation: Department of Obstetrics & Gynecology, Justus-Liebig-University, D-35385 Giessen, Germany.
authors.forename: Manuela
authors.initials: M
authors.lastname: Horndasch
authors.affiliation: Department of Obstetrics & Gynecology, Justus-Liebig-University, D-35385 Giessen, Germany; Department of Anesthesiology, Justus-Liebig-University, D-35385 Giessen, Germany.
authors.forename: Désirée
authors.initials: D
authors.lastname: Howe
authors.affiliation: Department of Obstetrics & Gynecology, Justus-Liebig-University, D-35385 Giessen, Germany; Institute for Clinical Immunology & Transfusion Medicine, Justus-Liebig-University, D-35385 Giessen, Germany.
authors.forename: Nelli
authors.initials: N
authors.lastname: Baal
authors.affiliation: Department of Obstetrics and Gynecology, Ernst-Moritz-Arndt-University, D-17475 Greifswald, Germany; Department of Biotechnology, School of Life Sciences, Central University of Rajasthan NH-8, Bandar Sindri, Distt. Ajmer, Rajasthan 305801, India.
authors.forename: Pankaj
authors.initials: P
authors.lastname: Goyal
authors.affiliation: Institute of Biochemistry, Medical School, Justus-Liebig-University, D-35392 Giessen, Germany.
authors.forename: Silvia
authors.initials: S
authors.lastname: Fischer
authors.affiliation: Department of Obstetrics and Gynecology, Ernst-Moritz-Arndt-University, D-17475 Greifswald, Germany.
authors.forename: Marek
authors.initials: M
authors.lastname: Zygmunt
authors.affiliation: Institute of Biochemistry, Medical School, Justus-Liebig-University, D-35392 Giessen, Germany. Electronic address: klaus.t.preissner@biochemie.med.uni-giessen.de.
authors.forename: Klaus T
authors.initials: KT
authors.lastname: Preissner
mesh_terms: D000071916:AC133 Antigen; D015703:Antigens, CD / Q000378:metabolism; D018952:Antigens, CD34 / Q000378:metabolism; D015153:Blotting, Western; D002448:Cell Adhesion / Q000502:physiology; D002465:Cell Movement / Q000502:physiology; D017931:DNA Primers / Q000235:genetics; D005260:Female; D005434:Flow Cytometry; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D006023:Glycoproteins / Q000378:metabolism; D006412:Hematopoietic Stem Cells / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism*; D018919:Neovascularization, Physiologic / Q000502:physiology*; D010455:Peptides / Q000378:metabolism; D010920:Placenta / Q000098:blood supply / Q000378:metabolism*; D010929:Placentation; D011247:Pregnancy; D011518:Proto-Oncogene Proteins / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D018709:Statistics, Nonparametric; D019010:Vascular Cell Adhesion Molecule-1 / Q000378:metabolism*; D051153:Wnt Proteins / Q000378:metabolism*; D060449:Wnt Signaling Pathway / Q000502:physiology*; D000071818:Wnt-5a Protein
chemical_list: D000071916:AC133 Antigen; D015703:Antigens, CD; D018952:Antigens, CD34; D017931:DNA Primers; D006023:Glycoproteins; C000606023:PROM1 protein, human; D010455:Peptides; D011518:Proto-Oncogene Proteins; D019010:Vascular Cell Adhesion Molecule-1; C085389:WNT5A protein, human; D051153:Wnt Proteins; D000071818:Wnt-5a Protein; D018799:Intercellular Adhesion Molecule-1
keywords: CD133(+)CD34(+) cells; Cell adhesion; Human trophoblast; Wnt-5a