title: The RSC complex localizes to coding sequences to regulate Pol II and histone occupancy.
abstract: ATP-dependent chromatin remodelers regulate chromatin structure during multiple stages of transcription. We report that RSC, an essential chromatin remodeler, is recruited to the open reading frames (ORFs) of actively transcribed genes genome wide, suggesting a role for RSC in regulating transcription elongation. Consistent with such a role, Pol II occupancy in the ORFs of weakly transcribed genes is drastically reduced upon depletion of the RSC catalytic subunit Sth1. RSC inactivation also reduced histone H3 occupancy across transcribed regions. Remarkably, the strongest effects on Pol II and H3 occupancy were confined to the genes displaying the greatest RSC ORF enrichment. Additionally, RSC recruitment to the ORF requires the activities of the SAGA and NuA4 HAT complexes and is aided by the activities of the Pol II CTD Ser2 kinases Bur1 and Ctk1. Overall, our findings strongly implicate ORF-associated RSC in governing Pol II function and in maintaining chromatin structure over transcribed regions.
authors.affiliation: Department of Biological Sciences, Oakland University, Rochester, MI 48309, USA.
authors.forename: Marla M
authors.initials: MM
authors.lastname: Spain
authors.affiliation: Laboratory of Molecular Genetics, Wadsworth Center, NY State Department of Health, Albany, NY 12208, USA.
authors.forename: Suraiya A
authors.initials: SA
authors.lastname: Ansari
authors.affiliation: Department of Biological Sciences, Oakland University, Rochester, MI 48309, USA.
authors.forename: Rakesh
authors.initials: R
authors.lastname: Pathak
authors.affiliation: Laboratory of Molecular Genetics, Wadsworth Center, NY State Department of Health, Albany, NY 12208, USA.
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Palumbo
authors.affiliation: Laboratory of Molecular Genetics, Wadsworth Center, NY State Department of Health, Albany, NY 12208, USA.
authors.forename: Randall H
authors.initials: RH
authors.lastname: Morse
authors.affiliation: Department of Biological Sciences, Oakland University, Rochester, MI 48309, USA. Electronic address: govind@oakland.edu.
authors.forename: Chhabi K
authors.initials: CK
authors.lastname: Govind
mesh_terms: D042002:Chromatin Assembly and Disassembly; D004268:DNA-Binding Proteins / Q000378:metabolism*; D015966:Gene Expression Regulation, Fungal; D006657:Histones / Q000378:metabolism*; D016366:Open Reading Frames; D012319:RNA Polymerase II / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism*; D013312:Stress, Physiological; D014157:Transcription Factors / Q000378:metabolism*; D014158:Transcription, Genetic
chemical_list: D004268:DNA-Binding Proteins; D006657:Histones; C103337:RSC complex, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; D012319:RNA Polymerase II