title: Differential effects of corticosterone on the colocalization of reelin and neuronal nitric oxide synthase in the adult hippocampus in wild type and heterozygous reeler mice.
abstract: Repeated corticosterone (CORT) treatment induces a deficit in dentate gyrus subgranular zone reelin-positive cells, in maturation of newborn neurons, and results in a consistent depressive-like behavior. However, the molecular mechanisms underlying these processes are not known in detail. The purpose of the present study was to characterize the effect of three weeks of 20mg/kg CORT injections in the number of reelin and neuronal nitric oxide synthase (nNOS), as well as their colocalization, in hippocampal regions in wild type (WTM) and heterozygous reeler mice (HRM). ANOVA analysis shows a CORT×genotype interaction in the density of reelin+ cells co-localizing nNOS in the dentate subgranular zone and stratum-lacunosum moleculare, and in the density of nNOS+ cells in the hilus. There is a main effect of CORT in the density of both reelin+ and nNOS+ cells in the dentate subgranular zone and hilus, and in reelin+ cells in the molecular layer and CA3 stratum radiatum; and a main effect of genotype on the co-localization of both markers in the dentate subgranular zone, and in the density of reelin+ cell sin the stratum lacunosum moleculare. These alterations suggest a possible interconnection between reelin and nNOS expression that is altered by repeated CORT treatment.
authors.affiliation: Department of Cell Biology, University of Santiago de Compostela, 15782 Santiago de Compostela, Galicia, Spain.
authors.forename: Raquel
authors.initials: R
authors.lastname: Romay-Tallon
authors.affiliation: Department of Cell Biology, University of Santiago de Compostela, 15782 Santiago de Compostela, Galicia, Spain.
authors.forename: Tania
authors.initials: T
authors.lastname: Rivera-Baltanas
authors.affiliation: Department of Medicine, University of Saskatchewan, Saskatoon, SK, Canada.
authors.forename: Lisa E
authors.initials: LE
authors.lastname: Kalynchuk
authors.affiliation: College of Pharmacy and Nutrition, University of Saskatchewan, Saskatoon, SK, Canada. Electronic address: hector.caruncho@usask.ca.
authors.forename: Hector J
authors.initials: HJ
authors.lastname: Caruncho
mesh_terms: D000818:Animals; D015816:Cell Adhesion Molecules, Neuronal / Q000235:genetics / Q000378:metabolism*; D003345:Corticosterone / Q000494:pharmacology*; D016326:Extracellular Matrix Proteins / Q000235:genetics / Q000378:metabolism*; D005838:Genotype; D006624:Hippocampus / Q000187:drug effects* / Q000378:metabolism*; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D008818:Mice, Neurologic Mutants; D018613:Microscopy, Confocal; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism*; D052248:Nitric Oxide Synthase Type I / Q000378:metabolism*; D000091042:Reelin Protein; D012697:Serine Endopeptidases / Q000235:genetics / Q000378:metabolism*
chemical_list: D015816:Cell Adhesion Molecules, Neuronal; D016326:Extracellular Matrix Proteins; D009419:Nerve Tissue Proteins; D000091042:Reelin Protein; D052248:Nitric Oxide Synthase Type I; C000717128:Reln protein, mouse; D012697:Serine Endopeptidases; D003345:Corticosterone
keywords: Hippocampus; Immunohistochemistry; Nitric oxide; Reelin deficits