title: Trypasonoma cruzi uses a specific subset of host cell lysosomes for cell invasion.
abstract: Trypanosoma cruzi is an intracellular parasite that depends on host cell lysosome recruitment and fusion for cell infection. Recently, we have shown that host cells present two differentially regulated lysosome pools. Treatment with methyl-beta cyclodextrin, a drug able to sequester cholesterol from plasma membrane, triggers the exocytosis of peripheral lysosomes, while treatment with Latrunculin-A, an actin depolymerizing drug, recruits a more internal pool. In this work we aimed to study which pool is used by the T. cruzi during invasion. We have shown that invasion is impaired when cells are previously treated with methyl-beta cyclodextrin, but not with Latrunculin-A, indicating that T. cruzi uses the cortical pool for invasion.
authors.affiliation: Departamento de Morfologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, BH, 31270-901, Brazil. Electronic address: barbarahissa@uchicago.edu.
authors.forename: Barbara
authors.initials: B
authors.lastname: Hissa
authors.affiliation: Departamento de Morfologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, BH, 31270-901, Brazil. Electronic address: lucianaandrade@ufmg.br.
authors.forename: Luciana de Oliveira
authors.initials: Lde O
authors.lastname: Andrade
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D019086:Bridged Bicyclo Compounds, Heterocyclic / Q000008:administration & dosage / Q000494:pharmacology; D002462:Cell Membrane; D008247:Lysosomes / Q000502:physiology*; D008561:Membrane Fusion; D051379:Mice; D032383:Myocytes, Cardiac / Q000469:parasitology*; D053778:Thiazolidines / Q000008:administration & dosage / Q000494:pharmacology; D014349:Trypanosoma cruzi / Q000502:physiology*; D047392:beta-Cyclodextrins / Q000008:administration & dosage / Q000494:pharmacology
chemical_list: D019086:Bridged Bicyclo Compounds, Heterocyclic; D053778:Thiazolidines; D047392:beta-Cyclodextrins; C108732:methyl-beta-cyclodextrin; C037067:latrunculin A
keywords: Trypanosoma cruzi; actin; cardiomyocytes; cholesterol; invasion; lysosomes