title: Methyl 3,4-dihydroxybenzoate extends the lifespan of Caenorhabditis elegans, partly via W06A7.4 gene.
abstract: To identify and analyze the compounds that delay aging and extend the lifespan is an important aspect of the gerontology research. A number of compounds, including the ones with the antioxidant properties, have been shown to extend the lifespan of Caenorhabditis elegans. Here, we report that methyl 3,4-dihydroxybenzoate (MDHB), a small antioxidant molecule, prolongs the C. elegans' lifespan under normal as well as stress conditions, delays the age-associated decline in the pharyngeal pumping rate, and obviously enhances the abilities of scavenging intracellular reactive oxygen species (ROS). To further investigate the mechanism underlying the anti-aging action of MDHB, microarray analyses were performed, which demonstrated that 13 genes were differentially expressed in worms treated with MDHB for 48 and 144 h in common. RNA interference of W06A7.4 (NM_001269697.1), the most significantly up-regulated gene, shortened the lifespan of worms by 14%, compared with the L4440 control. Our findings demonstrate that W06A7.4 is a potentially positive determinant of the MDHB induced C. elegans' lifespan extension effect.
authors.affiliation: Department of Pharmacology, School of Medicine, Jinan University, Guangzhou, China; Department of Pathogen Biology and Medical Immunology, School of Basic Medicine, Ningxia Medical University, Yinchuan, China; Discipline of Pathology and Pathophysiology, School of Medicine, Jinan University, Guangzhou, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Department of Pharmacology, School of Medicine, Jinan University, Guangzhou, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Cai
authors.affiliation: Department of Pharmacology, School of Medicine, Jinan University, Guangzhou, China.
authors.forename: Hai-Ju
authors.initials: HJ
authors.lastname: Geng
authors.affiliation: Department of Pharmacology, School of Medicine, Jinan University, Guangzhou, China.
authors.forename: Chao-Fen
authors.initials: CF
authors.lastname: Su
authors.affiliation: Department of Pharmacology, School of Medicine, Jinan University, Guangzhou, China.
authors.forename: Li
authors.initials: L
authors.lastname: Yan
authors.affiliation: Department of Pharmacology, School of Medicine, Jinan University, Guangzhou, China.
authors.forename: Jia-Hui
authors.initials: JH
authors.lastname: Wang
authors.affiliation: Department of Pharmacology, School of Medicine, Jinan University, Guangzhou, China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Gao
authors.affiliation: Department of Pharmacology, School of Medicine, Jinan University, Guangzhou, China; Institute of Brain Sciences, Jinan University, Guangzhou, China. Electronic address: tlhm@jnu.edu.cn.
authors.forename: Huan-Min
authors.initials: HM
authors.lastname: Luo
mesh_terms: D000375:Aging / Q000187:drug effects / Q000235:genetics / Q000502:physiology; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D017173:Caenorhabditis elegans / Q000187:drug effects* / Q000235:genetics* / Q000502:physiology; D017238:Genes, Helminth / Q000187:drug effects*; D062385:Hydroxybenzoates / Q000494:pharmacology*; D008136:Longevity / Q000187:drug effects* / Q000235:genetics* / Q000502:physiology; D034622:RNA Interference; D018510:RNA, Helminth / Q000235:genetics / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D059467:Transcriptome
chemical_list: D000975:Antioxidants; D062385:Hydroxybenzoates; D018510:RNA, Helminth; D017382:Reactive Oxygen Species; C000600341:methyl 3,4-dihydroxybenzoate
keywords: Aging; Caenorhabditis elegans; Lifespan; Methyl 3,4-dihydroxybenzoate; RNA interference; W06A7.4