title: CyTOF supports efficient detection of immune cell subsets from small samples.
abstract: Analysis of immune cell states is paramount to our understanding of the pathogenesis of a broad range of human diseases. Immunologists rely on fluorescence cytometry for cellular analysis, and while detection of 8 markers is now well established, the overlap of fluorescent signals limits efficiency. Mass cytometry or CyTOF (Cytometry by Time-Of-Flight) is a new technology for multiparameter single cell analysis that overcomes many limitations of fluorescence-based flow cytometry and can routinely detect as many as 40 markers per sample. This technology provides tremendous detail for cellular analysis of multiple cell populations simultaneously and is a powerful technique for translational investigations. Here we present reproducible detection of immune cell subsets starting with as few as 10,000 cells. Our study provides methods to employ CyTOF for small samples, which is especially relevant for investigation of limited patient biopsies in translational and clinical research.
authors.affiliation: Department of Internal Medicine, Yale University School of Medicine, New Haven, CT, United States.
authors.forename: Yi
authors.initials: Y
authors.lastname: Yao
authors.affiliation: Department of Immunobiology, Yale University School of Medicine, New Haven, CT, United States.
authors.forename: Rebecca
authors.initials: R
authors.lastname: Liu
authors.affiliation: Department of Internal Medicine, Yale University School of Medicine, New Haven, CT, United States.
authors.forename: Min Sun
authors.initials: MS
authors.lastname: Shin
authors.affiliation: Department of Internal Medicine, Yale University School of Medicine, New Haven, CT, United States.
authors.forename: Mark
authors.initials: M
authors.lastname: Trentalange
authors.affiliation: Department of Internal Medicine, Yale University School of Medicine, New Haven, CT, United States.
authors.forename: Heather
authors.initials: H
authors.lastname: Allore
authors.affiliation: Department of Internal Medicine, Yale University School of Medicine, New Haven, CT, United States.
authors.forename: Ala
authors.initials: A
authors.lastname: Nassar
authors.affiliation: Department of Internal Medicine, Yale University School of Medicine, New Haven, CT, United States.
authors.forename: Insoo
authors.initials: I
authors.lastname: Kang
authors.affiliation: Department of Internal Medicine, Yale University School of Medicine, New Haven, CT, United States; Department of Immunobiology, Yale University School of Medicine, New Haven, CT, United States; Human and Translational Immunology Program, Yale University School of Medicine, New Haven, CT, United States.
authors.forename: Jordan S
authors.initials: JS
authors.lastname: Pober
authors.affiliation: Department of Internal Medicine, Yale University School of Medicine, New Haven, CT, United States; Human and Translational Immunology Program, Yale University School of Medicine, New Haven, CT, United States. Electronic address: ruth.montgomery@yale.edu.
authors.forename: Ruth R
authors.initials: RR
authors.lastname: Montgomery
mesh_terms: D015703:Antigens, CD / Q000378:metabolism; D001402:B-Lymphocytes / Q000166:cytology* / Q000276:immunology / Q000378:metabolism; D015415:Biomarkers / Q000378:metabolism; D019070:Cell Lineage / Q000276:immunology; D003713:Dendritic Cells / Q000166:cytology* / Q000276:immunology / Q000378:metabolism; D005434:Flow Cytometry / Q000295:instrumentation / Q000379:methods; D006801:Humans; D016130:Immunophenotyping; D007694:Killer Cells, Natural / Q000166:cytology* / Q000276:immunology / Q000378:metabolism; D009000:Monocytes / Q000166:cytology* / Q000276:immunology / Q000378:metabolism; D059010:Single-Cell Analysis / Q000295:instrumentation / Q000379:methods; D012867:Skin / Q000166:cytology* / Q000276:immunology / Q000378:metabolism; D013194:Staining and Labeling / Q000379:methods; D013601:T-Lymphocytes / Q000166:cytology* / Q000276:immunology / Q000378:metabolism
chemical_list: D015703:Antigens, CD; D015415:Biomarkers
keywords: CyTOF; Leukocytes; Mass cytometry; Multidimensional single cell analysis; Translational immunology; Translational research