title: Co-culture of endothelial cells and patterned smooth muscle cells on titanium: construction with high density of endothelial cells and low density of smooth muscle cells.
abstract: Endothelialization has been considered a promising method to improve the biocompatibility of vascular implanted biomaterials. However, little is known about the anti-coagulation, anti-inflammatory, anti-atherosclerosis and anti-shedding property of the attached endothelial cells (ECs) and the relationship with their bio-environment and material-environment, which are both important evaluations to the cardiovascular biomaterials designed for tissue engineering applications and in vivo implantation. In this in vitro study, a novel co-culture model was built, where vascular smooth muscle cells (SMCs) were cultured on the hyaluronic acid (HA) micro-strip patterned titanium (Ti) surface on a low density to biomimetic the EC pericyte environment. Subsequently, the EC number and its functional factor, including nitric oxide (NO), prostacyclin (PGI2), tissue factor pathway inhibitor (TFPI), thrombomodulin (TM), and the inflammatory induced factor, endothelial leukocyte adhesion molecule-1 (E-selectin) were quantified, respectively. The anti-shedding property was also assessed by the blood flow shear stress (BFSS) acting. The results showed that the novel co-culture model possessed better EC coverage, functional factor release and anti-shedding functions than the control.
authors.affiliation: Key Lab. for Advanced Technologies of Materials, Ministry of Education, School of Material Science and Engineering, Southwest Jiaotong University, Chengdu 610031, PR China.
authors.forename: Jingan
authors.initials: J
authors.lastname: Li
authors.affiliation: Key Lab. for Advanced Technologies of Materials, Ministry of Education, School of Material Science and Engineering, Southwest Jiaotong University, Chengdu 610031, PR China; School of Life Science, Zhengzhou University, 100 Science Road, Zhengzhou 450001, PR China; Center of Stem Cell and Regenerative Medicine, First Affiliated Hospital of Zhengzhou University, 40 University Road, Zhengzhou 450052, PR China.
authors.forename: Kun
authors.initials: K
authors.lastname: Zhang
authors.affiliation: Key Lab. for Advanced Technologies of Materials, Ministry of Education, School of Material Science and Engineering, Southwest Jiaotong University, Chengdu 610031, PR China.
authors.forename: Juejue
authors.initials: J
authors.lastname: Wu
authors.affiliation: Key Lab. for Advanced Technologies of Materials, Ministry of Education, School of Material Science and Engineering, Southwest Jiaotong University, Chengdu 610031, PR China.
authors.forename: Yuzhen
authors.initials: Y
authors.lastname: Liao
authors.affiliation: Key Lab. for Advanced Technologies of Materials, Ministry of Education, School of Material Science and Engineering, Southwest Jiaotong University, Chengdu 610031, PR China. Electronic address: yangping8@263.net.
authors.forename: Ping
authors.initials: P
authors.lastname: Yang
authors.affiliation: Key Lab. for Advanced Technologies of Materials, Ministry of Education, School of Material Science and Engineering, Southwest Jiaotong University, Chengdu 610031, PR China.
authors.forename: Nan
authors.initials: N
authors.lastname: Huang
mesh_terms: D001672:Biocompatible Materials; D040761:Biomimetic Materials; D001807:Blood Vessel Prosthesis; D002318:Cardiovascular Diseases / Q000628:therapy; D002452:Cell Count; D018929:Cell Culture Techniques; D018920:Coculture Techniques; D019040:E-Selectin / Q000378:metabolism; D011464:Epoprostenol / Q000378:metabolism; D061307:Human Umbilical Vein Endothelial Cells / Q000166:cytology* / Q000378:metabolism; D006801:Humans; D008074:Lipoproteins / Q000378:metabolism; D032389:Myocytes, Smooth Muscle / Q000166:cytology* / Q000378:metabolism; D009569:Nitric Oxide / Q000378:metabolism; D020286:Pericytes / Q000166:cytology* / Q000378:metabolism; D033081:Shear Strength; D013314:Stress, Mechanical; D018180:Thrombomodulin / Q000378:metabolism; D023822:Tissue Engineering / Q000379:methods*; D014025:Titanium
chemical_list: D001672:Biocompatible Materials; D019040:E-Selectin; D008074:Lipoproteins; D018180:Thrombomodulin; C051928:lipoprotein-associated coagulation inhibitor; D009569:Nitric Oxide; D014025:Titanium; D011464:Epoprostenol
keywords: Biocompatibility; Cardiovascular implants; Co-culture; Endothelialization