title: MicroRNA-150 protects the heart from injury by inhibiting monocyte accumulation in a mouse model of acute myocardial infarction.
abstract: BACKGROUND
  MicroRNAs (miRs) and inflammatory monocytes participate in many cardiac pathophysiological processes including acute myocardial infarction (AMI). Recently, we observed that miR-150 is downregulated in injured mouse plasma after AMI as well as in human infarcted monocytes. However, the precise functional role of miR-150 in response to AMI remains unknown.
  
  
  RESULTS
  In a mouse model of AMI and in human subjects with AMI, we found that miR-150 expression was reduced in monocytes. In vitro studies showed that ectopic expression of miR-150 markedly reduced monocyte migration and proinflammatory cytokine production, whereas blockade of miR-150 had opposing effects. In vivo studies showed that overexpression of miR-150 in mice resulted in improved cardiac function, reduced myocardial infarction size, inhibition of apoptosis, and reduced inflammatory Ly-6C(high) monocyte invasion levels after AMI. Wild-type mice transplanted with miR-150 null (-/-) bone marrow cells could reverse this protective effect. Mechanistic studies demonstrated that miR-150 inhibited the expression of chemokine receptor 4 (CXCR4), thereby promoting monocyte migration.
  
  
  CONCLUSIONS
  Our findings indicate that miR-150 acts as a critical regulator of monocyte cell migration and production of proinflammatory cytokines, leading to cardioprotective effects against AMI-induced injury. Thus, miR-150 may be a suitable target for therapeutic intervention in the setting of ischemic heart disease.
authors.affiliation: From the Department of Thoracic Surgery, West China Hospital, Sichuan University, Chengdu, China (Z.L.); Department of Cardiovascular Surgery (Z.L., J.W., Y.S., A.Z., L.R., C.C., X.H., K.W., P.D., C.W., Z.Y., J.X.) and Department of Thoracic Surgery (S.W.), Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China; and Department of Cardiovascular Medicine (P.Y.) and Department of Cardiovascular Surgery (J.X.), Central Hospital of Wuhan, Wuhan, China.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Liu
authors.affiliation: From the Department of Thoracic Surgery, West China Hospital, Sichuan University, Chengdu, China (Z.L.); Department of Cardiovascular Surgery (Z.L., J.W., Y.S., A.Z., L.R., C.C., X.H., K.W., P.D., C.W., Z.Y., J.X.) and Department of Thoracic Surgery (S.W.), Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China; and Department of Cardiovascular Medicine (P.Y.) and Department of Cardiovascular Surgery (J.X.), Central Hospital of Wuhan, Wuhan, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Ye
authors.affiliation: From the Department of Thoracic Surgery, West China Hospital, Sichuan University, Chengdu, China (Z.L.); Department of Cardiovascular Surgery (Z.L., J.W., Y.S., A.Z., L.R., C.C., X.H., K.W., P.D., C.W., Z.Y., J.X.) and Department of Thoracic Surgery (S.W.), Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China; and Department of Cardiovascular Medicine (P.Y.) and Department of Cardiovascular Surgery (J.X.), Central Hospital of Wuhan, Wuhan, China.
authors.forename: Sihua
authors.initials: S
authors.lastname: Wang
authors.affiliation: From the Department of Thoracic Surgery, West China Hospital, Sichuan University, Chengdu, China (Z.L.); Department of Cardiovascular Surgery (Z.L., J.W., Y.S., A.Z., L.R., C.C., X.H., K.W., P.D., C.W., Z.Y., J.X.) and Department of Thoracic Surgery (S.W.), Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China; and Department of Cardiovascular Medicine (P.Y.) and Department of Cardiovascular Surgery (J.X.), Central Hospital of Wuhan, Wuhan, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Wu
authors.affiliation: From the Department of Thoracic Surgery, West China Hospital, Sichuan University, Chengdu, China (Z.L.); Department of Cardiovascular Surgery (Z.L., J.W., Y.S., A.Z., L.R., C.C., X.H., K.W., P.D., C.W., Z.Y., J.X.) and Department of Thoracic Surgery (S.W.), Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China; and Department of Cardiovascular Medicine (P.Y.) and Department of Cardiovascular Surgery (J.X.), Central Hospital of Wuhan, Wuhan, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Sun
authors.affiliation: From the Department of Thoracic Surgery, West China Hospital, Sichuan University, Chengdu, China (Z.L.); Department of Cardiovascular Surgery (Z.L., J.W., Y.S., A.Z., L.R., C.C., X.H., K.W., P.D., C.W., Z.Y., J.X.) and Department of Thoracic Surgery (S.W.), Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China; and Department of Cardiovascular Medicine (P.Y.) and Department of Cardiovascular Surgery (J.X.), Central Hospital of Wuhan, Wuhan, China.
authors.forename: Anchen
authors.initials: A
authors.lastname: Zhang
authors.affiliation: From the Department of Thoracic Surgery, West China Hospital, Sichuan University, Chengdu, China (Z.L.); Department of Cardiovascular Surgery (Z.L., J.W., Y.S., A.Z., L.R., C.C., X.H., K.W., P.D., C.W., Z.Y., J.X.) and Department of Thoracic Surgery (S.W.), Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China; and Department of Cardiovascular Medicine (P.Y.) and Department of Cardiovascular Surgery (J.X.), Central Hospital of Wuhan, Wuhan, China.
authors.forename: Linyun
authors.initials: L
authors.lastname: Ren
authors.affiliation: From the Department of Thoracic Surgery, West China Hospital, Sichuan University, Chengdu, China (Z.L.); Department of Cardiovascular Surgery (Z.L., J.W., Y.S., A.Z., L.R., C.C., X.H., K.W., P.D., C.W., Z.Y., J.X.) and Department of Thoracic Surgery (S.W.), Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China; and Department of Cardiovascular Medicine (P.Y.) and Department of Cardiovascular Surgery (J.X.), Central Hospital of Wuhan, Wuhan, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Cheng
authors.affiliation: From the Department of Thoracic Surgery, West China Hospital, Sichuan University, Chengdu, China (Z.L.); Department of Cardiovascular Surgery (Z.L., J.W., Y.S., A.Z., L.R., C.C., X.H., K.W., P.D., C.W., Z.Y., J.X.) and Department of Thoracic Surgery (S.W.), Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China; and Department of Cardiovascular Medicine (P.Y.) and Department of Cardiovascular Surgery (J.X.), Central Hospital of Wuhan, Wuhan, China.
authors.forename: Xiaofan
authors.initials: X
authors.lastname: Huang
authors.affiliation: From the Department of Thoracic Surgery, West China Hospital, Sichuan University, Chengdu, China (Z.L.); Department of Cardiovascular Surgery (Z.L., J.W., Y.S., A.Z., L.R., C.C., X.H., K.W., P.D., C.W., Z.Y., J.X.) and Department of Thoracic Surgery (S.W.), Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China; and Department of Cardiovascular Medicine (P.Y.) and Department of Cardiovascular Surgery (J.X.), Central Hospital of Wuhan, Wuhan, China.
authors.forename: Ke
authors.initials: K
authors.lastname: Wang
authors.affiliation: From the Department of Thoracic Surgery, West China Hospital, Sichuan University, Chengdu, China (Z.L.); Department of Cardiovascular Surgery (Z.L., J.W., Y.S., A.Z., L.R., C.C., X.H., K.W., P.D., C.W., Z.Y., J.X.) and Department of Thoracic Surgery (S.W.), Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China; and Department of Cardiovascular Medicine (P.Y.) and Department of Cardiovascular Surgery (J.X.), Central Hospital of Wuhan, Wuhan, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Deng
authors.affiliation: From the Department of Thoracic Surgery, West China Hospital, Sichuan University, Chengdu, China (Z.L.); Department of Cardiovascular Surgery (Z.L., J.W., Y.S., A.Z., L.R., C.C., X.H., K.W., P.D., C.W., Z.Y., J.X.) and Department of Thoracic Surgery (S.W.), Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China; and Department of Cardiovascular Medicine (P.Y.) and Department of Cardiovascular Surgery (J.X.), Central Hospital of Wuhan, Wuhan, China.
authors.forename: Chuangyan
authors.initials: C
authors.lastname: Wu
authors.affiliation: From the Department of Thoracic Surgery, West China Hospital, Sichuan University, Chengdu, China (Z.L.); Department of Cardiovascular Surgery (Z.L., J.W., Y.S., A.Z., L.R., C.C., X.H., K.W., P.D., C.W., Z.Y., J.X.) and Department of Thoracic Surgery (S.W.), Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China; and Department of Cardiovascular Medicine (P.Y.) and Department of Cardiovascular Surgery (J.X.), Central Hospital of Wuhan, Wuhan, China.
authors.forename: Zhang
authors.initials: Z
authors.lastname: Yue
authors.affiliation: From the Department of Thoracic Surgery, West China Hospital, Sichuan University, Chengdu, China (Z.L.); Department of Cardiovascular Surgery (Z.L., J.W., Y.S., A.Z., L.R., C.C., X.H., K.W., P.D., C.W., Z.Y., J.X.) and Department of Thoracic Surgery (S.W.), Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China; and Department of Cardiovascular Medicine (P.Y.) and Department of Cardiovascular Surgery (J.X.), Central Hospital of Wuhan, Wuhan, China. jiahong.xia@mail.hust.edu.cn.
authors.forename: Jiahong
authors.initials: J
authors.lastname: Xia
mesh_terms: D000818:Animals; D002465:Cell Movement; D004195:Disease Models, Animal; D005786:Gene Expression Regulation; D006801:Humans; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D035683:MicroRNAs / Q000096:biosynthesis* / Q000235:genetics; D009000:Monocytes / Q000378:metabolism* / Q000473:pathology; D009203:Myocardial Infarction / Q000235:genetics / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*
chemical_list: D035683:MicroRNAs; C533097:MIR150, human; C529101:Mirn150 microRNA, mouse
keywords: acute myocardial infarction; microRNAs; monocytes