title: Antibacterial activity of antagonistic bacterium Bacillus subtilis DJM-51 against phytopathogenic Clavibacter michiganense subsp. michiganense ATCC 7429 in vitro.
abstract: To investigate antibacterial activity against the tomato pathogen Clavibacter michiganense subsp. michiganense ATCC 7429 (Cmm ATCC 7429), Bacillus subtilis DJM-51 was isolated from rhizosphere soil. For isolation of bacteria, samples were taken from rhizosphere soil. The isolate, DJA-51, had strong antagonistic ability against Tomato pathogen Cmm ATCC 7429 on nutrient-broth yeast extract agar (NBYA) as indicated by inhibition zones around colonies. On the basis of the nucleotide sequence of a conserved segment of the 16S rRNA gene, the bacterium has been identified as B. subtilis DJM-51. The growth of Cmm ATCC 7429 on NBYA plates was inhibited by culture broth of B. subtilis DJM-51 including cells, by the supernatant of culture broth of B. subtilis DJM-51, and by the liquid material resulting from butanol extract of bacterial cultures. The OD value in co-culture mixture was lower than the control throughout the entire incubation period. Antibiotics obtained from B. subtilis DJM-51 inhibited the growth of Tomato pathogen Cmm ATCC 7429. These results provide potentially information about the protection of tomato from pathogen Cmm ATCC 7429 under greenhouse conditions in Quebec.
authors.affiliation: Division of Applied Bioscience and Biotechnology, Institute of Environmentally-Friendly Agriculture (IEFA), College of Agricultural and Life Science, Chonnam National University, Gwangju 500-757, Republic of Korea. Electronic address: woojung@chonnam.ac.kr.
authors.forename: W J
authors.initials: WJ
authors.lastname: Jung
authors.affiliation: Department of Plant Science, Macdonald Campus of McGill University, 21,111 Lakeshore Road, St Anne-de-Bellevue, Quebec H9X 3V9, Canada.
authors.forename: F
authors.initials: F
authors.lastname: Mabood
authors.affiliation: Department of Plant Science, Macdonald Campus of McGill University, 21,111 Lakeshore Road, St Anne-de-Bellevue, Quebec H9X 3V9, Canada.
authors.forename: A
authors.initials: A
authors.lastname: Souleimanov
authors.affiliation: Department of Natural Resource Science, Microbiology, Macdonald Campus of McGill University, 21,111 Lakeshore Road, St Anne-de-Bellevue, Quebec H9X 3V9, Canada.
authors.forename: L G
authors.initials: LG
authors.lastname: Whyte
authors.affiliation: Department of Natural Resource Science, Microbiology, Macdonald Campus of McGill University, 21,111 Lakeshore Road, St Anne-de-Bellevue, Quebec H9X 3V9, Canada.
authors.forename: T D
authors.initials: TD
authors.lastname: Niederberger
authors.affiliation: Department of Plant Science, Macdonald Campus of McGill University, 21,111 Lakeshore Road, St Anne-de-Bellevue, Quebec H9X 3V9, Canada.
authors.forename: D L
authors.initials: DL
authors.lastname: Smith
mesh_terms: D039903:Actinobacteria / Q000187:drug effects / Q000254:growth & development*; D000898:Antibiosis; D001412:Bacillus subtilis / Q000254:growth & development / Q000302:isolation & purification / Q000502:physiology*; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D018551:Solanum lycopersicum / Q000382:microbiology; D008828:Microbiological Techniques; D008969:Molecular Sequence Data; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D058441:Rhizosphere; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S
keywords: Antibacterial activity; Bacillus subtilis; Clavibacter michiganense subsp. michiganense ATCC 7429; Rhizosphere