title: Hypotensive effects of omentin-1 related to increased adiponectin and decreased interleukin-6 in intra-thoracic pericardial adipose tissue.
abstract: BACKGROUND
  Omentin is an adipokine expressed in visceral adipose tissue (VAT). In vitro studies demonstrated that omentin induces vasorelaxation in isolated rat mesenteric arteries, and in vivo studies showed inhibition of agonist-induced increases in blood pressure, possibly mediated by nitric oxide (NO)-dependent mechanisms.
  
  
  METHODS
  We investigated, in normotensive rats, the effects of subacute omentin-1 administration [8μg/kg, intraperitoneally (ip), once daily for 14 days] on cardiac activity, blood pressure, plasma concentration of l-citrulline (as a marker of NO production from l-arginine), and the gene expression of adiponectin, tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) in intra-thoracic pericardial adipose tissue (PAT). Electrocardiography (ECG), heart rate (HR), mean blood pressure (MBP), pulse pressure (PP) were monitored before and after treatment with omentin-1 or vehicle.
  
  
  RESULTS
  With respect to baseline and vehicle, we found a significant decrease of MBP (p<0.005) and PP (p<0.05) after treatment with omentin-1, while ECG and HR were not modified. Omentin-1 significantly increased l-citrulline levels in plasma (p<0.05), and the gene expression of adiponectin in PAT (p<0.05). On the other hand, we found decreased gene expression of IL-6 (p<0.005), while TNF-α mRNA in PAT was not affected.
  
  
  CONCLUSIONS
  We conclude that the hypotensive effects of omentin-1 could be driven by stimulated production of NO in the vascular system, possibly related to increased adiponectin and decreased IL-6 mRNA in PAT.
authors.affiliation: Department of Pharmacy, "G. d'Annunzio" University, Chieti, Italy.
authors.forename: Luigi
authors.initials: L
authors.lastname: Brunetti
authors.affiliation: Department of Pharmacy, "G. d'Annunzio" University, Chieti, Italy.
authors.forename: Sheila
authors.initials: S
authors.lastname: Leone
authors.affiliation: Department of Pharmacy, "G. d'Annunzio" University, Chieti, Italy.
authors.forename: Giustino
authors.initials: G
authors.lastname: Orlando
authors.affiliation: Department of Pharmacy, "G. d'Annunzio" University, Chieti, Italy.
authors.forename: Claudio
authors.initials: C
authors.lastname: Ferrante
authors.affiliation: Department of Pharmacy, "G. d'Annunzio" University, Chieti, Italy.
authors.forename: Lucia
authors.initials: L
authors.lastname: Recinella
authors.affiliation: Department of Pharmacy, "G. d'Annunzio" University, Chieti, Italy.
authors.forename: Annalisa
authors.initials: A
authors.lastname: Chiavaroli
authors.affiliation: Department of Pharmacy, "G. d'Annunzio" University, Chieti, Italy.
authors.forename: Chiara
authors.initials: C
authors.lastname: Di Nisio
authors.affiliation: Department of Pharmacy, "G. d'Annunzio" University, Chieti, Italy.
authors.forename: Rugia
authors.initials: R
authors.lastname: Shohreh
authors.affiliation: Department of Pharmacy, "G. d'Annunzio" University, Chieti, Italy.
authors.forename: Fabio
authors.initials: F
authors.lastname: Manippa
authors.affiliation: Department of Pharmacy, "G. d'Annunzio" University, Chieti, Italy.
authors.forename: Adriana
authors.initials: A
authors.lastname: Ricciuti
authors.affiliation: Department of Pharmacy, "G. d'Annunzio" University, Chieti, Italy. Electronic address: mvacca@unich.it.
authors.forename: Michele
authors.initials: M
authors.lastname: Vacca
mesh_terms: D052242:Adiponectin / Q000235:genetics*; D000273:Adipose Tissue / Q000187:drug effects* / Q000378:metabolism; D000818:Animals; D001794:Blood Pressure / Q000187:drug effects; D002956:Citrulline / Q000097:blood; D016207:Cytokines / Q000008:administration & dosage / Q000494:pharmacology*; D004562:Electrocardiography; D058851:GPI-Linked Proteins / Q000008:administration & dosage / Q000494:pharmacology; D005786:Gene Expression Regulation / Q000187:drug effects; D006339:Heart Rate / Q000187:drug effects; D015850:Interleukin-6 / Q000235:genetics; D037102:Lectins / Q000008:administration & dosage / Q000494:pharmacology*; D009569:Nitric Oxide / Q000378:metabolism; D010496:Pericardium / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics
chemical_list: D052242:Adiponectin; D016207:Cytokines; D058851:GPI-Linked Proteins; C432397:ITLN1 protein, human; D015850:Interleukin-6; D037102:Lectins; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; D002956:Citrulline; D009569:Nitric Oxide
keywords: Adiponectin; Hypotension; Interleukin-6; Omentin-1; l-Citrulline