title: Molecular recognition in the interaction of chloroplast 2-Cys peroxiredoxin with NADPH-thioredoxin reductase C (NTRC) and thioredoxin x.
abstract: In addition to the standard NADPH thioredoxin reductases (NTRs), plants hold a plastidic NTR (NTRC), with a thioredoxin module fused at the C-terminus. NTRC is an efficient reductant of 2-Cys peroxiredoxins (2-Cys Prxs). The interaction of NTRC and chloroplastic thioredoxin x with 2-Cys Prxs has been confirmed in vivo, by bimolecular fluorescence complementation (BiFC) assays, and in vitro, by isothermal titration calorimetry (ITC) experiments. In comparison with thioredoxin x, NTRC interacts with 2-Cys Prx with higher affinity, both the thioredoxin and NTR domains of NTRC contributing significantly to this interaction, as demonstrated by using the NTR and thioredoxin modules of the enzyme expressed separately. The presence of the thioredoxin domain seems to prevent the interaction of NTRC with thioredoxin x.
authors.affiliation: Instituto de Bioquímica Vegetal y Fotosíntesis, CSIC and Universidad de Sevilla, Avda. Américo Vespucio 49, 41092 Sevilla, Spain.
authors.forename: Pilar
authors.initials: P
authors.lastname: Bernal-Bayard
authors.affiliation: Instituto de Bioquímica Vegetal y Fotosíntesis, CSIC and Universidad de Sevilla, Avda. Américo Vespucio 49, 41092 Sevilla, Spain.
authors.forename: Valle
authors.initials: V
authors.lastname: Ojeda
authors.affiliation: Instituto de Bioquímica Vegetal y Fotosíntesis, CSIC and Universidad de Sevilla, Avda. Américo Vespucio 49, 41092 Sevilla, Spain.
authors.forename: Manuel
authors.initials: M
authors.lastname: Hervás
authors.affiliation: Instituto de Bioquímica Vegetal y Fotosíntesis, CSIC and Universidad de Sevilla, Avda. Américo Vespucio 49, 41092 Sevilla, Spain.
authors.forename: Francisco J
authors.initials: FJ
authors.lastname: Cejudo
authors.affiliation: Instituto de Bioquímica Vegetal y Fotosíntesis, CSIC and Universidad de Sevilla, Avda. Américo Vespucio 49, 41092 Sevilla, Spain. Electronic address: jnavarro@ibvf.csic.es.
authors.forename: José A
authors.initials: JA
authors.lastname: Navarro
authors.affiliation: Institute of Biocomputation and Physics of Complex Systems (BIFI), Joint Unit IQFR-CSIC-BIFI, Universidad de Zaragoza, C/Mariano Esquillor, Zaragoza 50018, Spain; Department of Biochemistry and Molecular and Cell Biology, University of Zaragoza, Zaragoza, Spain; Fundacion ARAID, Government of Aragon, Spain.
authors.forename: Adrián
authors.initials: A
authors.lastname: Velázquez-Campoy
authors.affiliation: Instituto de Bioquímica Vegetal y Fotosíntesis, CSIC and Universidad de Sevilla, Avda. Américo Vespucio 49, 41092 Sevilla, Spain.
authors.forename: Juan M
authors.initials: JM
authors.lastname: Pérez-Ruiz
mesh_terms: D017360:Arabidopsis / Q000166:cytology* / Q000201:enzymology*; D029681:Arabidopsis Proteins / Q000378:metabolism*; D002736:Chloroplasts / Q000378:metabolism*; D054464:Peroxiredoxins / Q000378:metabolism*; D011485:Protein Binding; D013880:Thioredoxin-Disulfide Reductase / Q000378:metabolism*; D013879:Thioredoxins / Q000378:metabolism*
chemical_list: D029681:Arabidopsis Proteins; D013879:Thioredoxins; C493631:2-Cys peroxiredoxin A, Arabidopsis; D054464:Peroxiredoxins; D013880:Thioredoxin-Disulfide Reductase
keywords: Bimolecular fluorescence complementation; Chloroplast NADPH-dependent thioredoxin-reductase; Isothermal titration calorimetry; Molecular recognition; NTRC; Peroxiredoxin; Thioredoxin