title: Association of CRISPR/Cas evolution with Vibrio parahaemolyticus virulence factors and genotypes.
abstract: Clustered regularly interspaced short palindromic repeats (CRISPR), which is considered to be an immune system for bacteria, has been widely used as a tool for genome editing and genotyping. It has also been reported to be associated with virulence factors in some bacteria. To understand the role of CRISPR in the virulence and evolution of pathogenic Vibrio parahaemolyticus, 154 V. parahaemolyticus strains isolated from clinical samples and 54 strains from food samples taken in Shenzhen, China were subjected to a correlation analysis of CRISPR and virulence factors TDH and TRH. We also performed multilocus sequence typing (MLST) for genotype analysis. Six different CRISPR sequence types (CSTs) of V. parahaemolyticus were identified, and CSTs were found to be significantly associated with the virulence factors tested and MLST genotype. Therefore, CSTs provide insight into the evolution of V. parahaemolyticus. Moreover, identification of CSTs may lend insight into the virulence potential of strains.
authors.affiliation: 1 Key Laboratory of Bio-resources and Eco-environment of the Ministry of Education, College of Life Sciences, Sichuan University , Chengdu, Sichuan, China .
authors.forename: Honghu
authors.initials: H
authors.lastname: Sun
authors.forename: Yinghui
authors.initials: Y
authors.lastname: Li
authors.forename: Xiaolu
authors.initials: X
authors.lastname: Shi
authors.forename: Yiman
authors.initials: Y
authors.lastname: Lin
authors.forename: Yaqun
authors.initials: Y
authors.lastname: Qiu
authors.forename: Jinjin
authors.initials: J
authors.lastname: Zhang
authors.forename: Yao
authors.initials: Y
authors.lastname: Liu
authors.forename: Min
authors.initials: M
authors.lastname: Jiang
authors.forename: Zhen
authors.initials: Z
authors.lastname: Zhang
authors.forename: Qiongcheng
authors.initials: Q
authors.lastname: Chen
authors.forename: Qun
authors.initials: Q
authors.lastname: Sun
authors.forename: Qinghua
authors.initials: Q
authors.lastname: Hu
mesh_terms: D015373:Bacterial Typing Techniques; D064113:CRISPR-Cas Systems; D002681:China; D064112:Clustered Regularly Interspaced Short Palindromic Repeats / Q000235:genetics*; D016521:Electrophoresis, Gel, Pulsed-Field; D005838:Genotype; D006801:Humans; D058885:Multilocus Sequence Typing; D012703:Serotyping; D014736:Vibrio parahaemolyticus / Q000145:classification / Q000235:genetics*; D037521:Virulence Factors / Q000235:genetics*
chemical_list: D037521:Virulence Factors