title: Temporal analysis of prostaglandin F2α receptor, caspase 3, and cyclooxygenase 2 messenger RNA expression and prostaglandin F2α receptor and cyclooxygenase 2 protein expression in endometrial tissue from multiparous Nelore (Bos taurus indicus) cows treated with cloprostenol sodium during puerperium.
abstract: The use of cloprostenol sodium in puerperium is questionable, as both favorable and unfavorable responses during the uterine involution process have been reported in the literature. This study is based on the hypothesis that cloprostenol sodium promotes modifications in the prostaglandin F2α receptor (FP), caspase 3 (CASP-3), and cyclooxygenase 2 (COX-2) mRNA expression that may favor the process of postpartum uterine involution in multiparous Nelore (Bos taurus indicus) females. Additionally, we aimed to describe the presence and immunolocalization of the FP and COX-2 protein in endometrial tissue at different postpartum time points in these females. Multiparous Nelore cows (n = 24) were treated with cloprostenol sodium (n = 12) or saline solution (n = 12) on postpartum Days 1 and 4 (Day 0 = birth), and endometrial biopsies were performed with a Yomann biopsy instrument and collected on Days 1, 7, 14, 28, and 42 postpartum. The mRNA expression from samples on the Days 1, 7, 14, 28, and 42 and the protein expression from samples on the Days 1, 14, 28, and 42 were then analyzed. The treated cows had altered FP and CASP-3 mRNA expression, and FP and COX-2 protein were observed in the endometrial surface epithelium, the stroma, and the glandular epithelium, with cytoplasmic immunolocalization. Although we attribute the change in CASP-3 mRNA expression to physiological phenomena, the results obtained for FP mRNA expression opens new doors for the study of hormonal protocols associated with cloprostenol sodium in the puerperium of Zebu females.
authors.affiliation: Department of Animal Reproduction and Radiology, São Paulo State University-UNESP, Botucatu, São Paulo, Brazil. Electronic address: carolnmoraes@hotmail.com.
authors.forename: Carolina N
authors.initials: CN
authors.lastname: de Moraes
authors.affiliation: Department of Animal Reproduction and Radiology, São Paulo State University-UNESP, Botucatu, São Paulo, Brazil.
authors.forename: Leandro
authors.initials: L
authors.lastname: Maia
authors.affiliation: Department of Pharmacology, Biosciences Institute, São Paulo State University-UNESP, Botucatu, São Paulo, Brazil.
authors.forename: Paula F
authors.initials: PF
authors.lastname: de Lima
authors.affiliation: Department of Animal Reproduction and Radiology, São Paulo State University-UNESP, Botucatu, São Paulo, Brazil.
authors.forename: Marianne C
authors.initials: MC
authors.lastname: Dias
authors.affiliation: Department of Medical Clinic, São Paulo State University-UNESP, Jaboticabal, São Paulo, Brazil.
authors.forename: Talita M M
authors.initials: TM
authors.lastname: Raposo-Ferreira
authors.affiliation: Laboratory of Genetics and Animal Breeding, Federal University of Pampa-UNIPAMPA, Uruguaiana, Rio Grande do Sul, Brazil.
authors.forename: Mateus J
authors.initials: MJ
authors.lastname: Sudano
authors.affiliation: Department of Pharmacology, Biosciences Institute, São Paulo State University-UNESP, Botucatu, São Paulo, Brazil.
authors.forename: José B
authors.initials: JB
authors.lastname: Junior
authors.affiliation: Department of Animal Reproduction and Radiology, São Paulo State University-UNESP, Botucatu, São Paulo, Brazil.
authors.forename: Eunice
authors.initials: E
authors.lastname: Oba
mesh_terms: D000818:Animals; D000971:Antineoplastic Combined Chemotherapy Protocols; D001706:Biopsy / Q000662:veterinary; D053148:Caspase 3 / Q000235:genetics*; D002417:Cattle; D003008:Cloprostenol / Q000008:administration & dosage*; D051546:Cyclooxygenase 2 / Q000032:analysis / Q000235:genetics*; D004717:Endometrium / Q000737:chemistry / Q000187:drug effects*; D005047:Etoposide; D005260:Female; D015870:Gene Expression / Q000187:drug effects; D007069:Ifosfamide; D007150:Immunohistochemistry; D008186:Luteolytic Agents; D008727:Methotrexate; D049590:Postpartum Period; D012333:RNA, Messenger / Q000032:analysis; D011982:Receptors, Prostaglandin / Q000032:analysis / Q000235:genetics*
chemical_list: D008186:Luteolytic Agents; D012333:RNA, Messenger; D011982:Receptors, Prostaglandin; C034671:prostaglandin F2alpha receptor; D003008:Cloprostenol; D005047:Etoposide; D051546:Cyclooxygenase 2; D053148:Caspase 3; D007069:Ifosfamide; D008727:Methotrexate
keywords: Bovine; Cloprostenol sodium; Immunohistochemistry; Prostaglandin F2α receptor; Puerperium