title: Texosome-anchored superantigen triggers apoptosis in original ovarian cancer cells.
abstract: Texosomes, nano-endosomal vesicles, are candidates for cancer immunotherapy due to their immunostimulating properties. We designed a new structure based on texosome and staphylococcal enterotoxin B (SEB) and assessed its cytotoxic impact on an ovarian cell line. Texosomes were isolated from tumor cells, and SEB was anchored onto by protein transfer method. MTT assay and Hoechst staining were used to identify the cytotoxic and apoptotic effects of this compound on treated cells with different concentrations of texosome-SEB (TEX-SEB). Moreover, the expression rate of bcl-2, bax, bak, bcl-xl and the activity of caspase-3 and caspase-9 were investigated. Treatments of the cells with 0.5, 2.5 and 10 μg/100 μl TEX-SEB were significantly cytotoxic within 24 h (p < 0.001). Hoechst staining revealed that all tested concentrations caused apoptosis after 24 h compared with the control cells (p < 0.001). Furthermore, it was found that treatment with all examined concentrations of TEX-SEB enhanced caspase-9 activity after 24 and 48 h, while caspase-3 activity was increased upon treatment with only 0.5 and 2.5 μg/100 μl of TEX-SEB after 24 h (p < 0.001). None of the concentrations of TEX-SEB affected the expression of the cancer-promoting genes. Our construct, the TEX-SEB, is a new model being able to create cytostatic properties on cancer cells.
authors.affiliation: Applied Microbiology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran.
authors.forename: Hamideh
authors.initials: H
authors.lastname: Mahmoodzadeh Hosseini
authors.forename: Jafar
authors.initials: J
authors.lastname: Soleimanirad
authors.forename: Elnaz
authors.initials: E
authors.lastname: Mehdizadeh Aghdam
authors.forename: Mohsen
authors.initials: M
authors.lastname: Amin
authors.forename: Abbas Ali
authors.initials: AA
authors.lastname: Imani Fooladi
mesh_terms: D017209:Apoptosis / Q000276:immunology*; D015153:Blotting, Western; D045744:Cell Line, Tumor; D004768:Enterotoxins / Q000008:administration & dosage* / Q000276:immunology; D005260:Female; D006801:Humans; D007167:Immunotherapy / Q000379:methods*; D046529:Microscopy, Electron, Transmission; D057146:Multivesicular Bodies; D010051:Ovarian Neoplasms / Q000276:immunology / Q000473:pathology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D059467:Transcriptome
chemical_list: D004768:Enterotoxins; C031818:enterotoxin B, staphylococcal