title: Germ line development: lessons learned from pluripotent stem cells.
abstract: Current knowledge about mammalian germ line development is mainly based on the mouse model and little is known about how this fundamental process occurs in humans. This review summarizes our current knowledge of genetic and epigenetic germ line development in mammals, mainly focusing on primordial germ cell (PGC) specification events, comparing the differences between mouse and human models. We also emphasize the knowledge derived from the most successful strategies used to generate germ cell-like cells in vitro in both models and major obstacles to obtaining bona fide in vitro-derived gametes are considered.
authors.affiliation: Fundación Instituto Valenciano de Infertilidad (FIVI), Dept. Obst. & Gynec., Valencia University, Valencia, Spain; INCLIVA Biomedical Research Institute, Valencia 46015, Spain.
authors.forename: Ana M
authors.initials: AM
authors.lastname: Martínez-Arroyo
authors.affiliation: Fundación Instituto Valenciano de Infertilidad (FIVI), Dept. Obst. & Gynec., Valencia University, Valencia, Spain; Fundación Instituto de Investigación Sanitaria La Fe, Valencia 46026, Spain.
authors.forename: Jose V
authors.initials: JV
authors.lastname: Medrano
authors.affiliation: Fundación Instituto Valenciano de Infertilidad (FIVI), Dept. Obst. & Gynec., Valencia University, Valencia, Spain; Fundación Instituto de Investigación Sanitaria La Fe, Valencia 46026, Spain.
authors.forename: José
authors.initials: J
authors.lastname: Remohí
authors.affiliation: Fundación Instituto Valenciano de Infertilidad (FIVI), Dept. Obst. & Gynec., Valencia University, Valencia, Spain; INCLIVA Biomedical Research Institute, Valencia 46015, Spain; Department of Obstetrics and Gynecology, Stanford University, Stanford, CA 94305, USA. Electronic address: carlos.simon@ivi.es.
authors.forename: Carlos
authors.initials: C
authors.lastname: Simón
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D053595:Embryonic Stem Cells / Q000166:cytology*; D005854:Germ Cells / Q000166:cytology*; D006801:Humans; D051379:Mice; D039904:Pluripotent Stem Cells / Q000166:cytology*