title: Imidazoline derivative templated synthesis of broccoli-like Bi2S3 and its electrocatalysis towards the direct electrochemistry of hemoglobin.
abstract: A broccoli-like bismuth sulfide (bBi2S3) was synthesized via a solvothermal method using a self-made imidazoline derivative of 2-undecyl-1-dithioureido-ethyl-imidazoline as the soft template. The morphology and chemical constitution of the product were characterized by scanning electron microscope (SEM), transmission electron microscope (TEM) and X-ray diffraction (XRD). Electrochemical characterization experiments show that the bBi2S3 has the higher specific surface area and standard heterogeneous electron transfer rate constant than the rod-like Bi2S3 (rBi2S3). Hemoglobin (Hb) was then chosen as a protein model to investigate the electrocatalytic property of the synthesized bBi2S3. The results show that Hb entrapped in the composite film of chitosan and bBi2S3 displays an excellent direct electrochemistry, and retains its biocatalytic activity toward the electro-reduction of hydrogen peroxide. The current response in the amperometry shows a linear response to H2O2 concentrations in the range from 0.4 to 4.8µM with high sensitivity (444µAmM(-1)) and low detection limit (0.096µM). The Michaelis-Menten constant (KM(app)) of the fabricated bioelectrode for H2O2 was determined as low as 1µM. These results demonstrate that the synthesized bBi2S3 offers a new path for the immobilization of redox-active protein and the construction of the third-generation biosensors.
authors.affiliation: College of Chemistry and Environment, Fujian Province Key Laboratory of Morden Analytical Science and Separation Technology, Minnan Normal University, Zhangzhou 363000, PR China.
authors.forename: Xiaoqian
authors.initials: X
authors.lastname: Chen
authors.affiliation: College of Chemistry and Environment, Fujian Province Key Laboratory of Morden Analytical Science and Separation Technology, Minnan Normal University, Zhangzhou 363000, PR China. Electronic address: axiang236@126.com.
authors.forename: Qingxiang
authors.initials: Q
authors.lastname: Wang
authors.affiliation: College of Chemistry and Environment, Fujian Province Key Laboratory of Morden Analytical Science and Separation Technology, Minnan Normal University, Zhangzhou 363000, PR China.
authors.forename: Liheng
authors.initials: L
authors.lastname: Wang
authors.affiliation: College of Chemistry and Environment, Fujian Province Key Laboratory of Morden Analytical Science and Separation Technology, Minnan Normal University, Zhangzhou 363000, PR China.
authors.forename: Feng
authors.initials: F
authors.lastname: Gao
authors.affiliation: College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.affiliation: College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, China.
authors.forename: Zhengshui
authors.initials: Z
authors.lastname: Hu
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D002384:Catalysis; D048271:Chitosan / Q000737:chemistry; D004563:Electrochemistry; D006454:Hemoglobins / Q000737:chemistry / Q000302:isolation & purification*; D006801:Humans; D006861:Hydrogen Peroxide / Q000737:chemistry; D048288:Imidazolines / Q000138:chemical synthesis / Q000737:chemistry*; D055767:Immobilized Proteins / Q000737:chemistry; D014961:X-Ray Diffraction
chemical_list: D006454:Hemoglobins; D048288:Imidazolines; D055767:Immobilized Proteins; D048271:Chitosan; D006861:Hydrogen Peroxide
keywords: Bi(2)S(3); Direct electrochemistry; Hemoglobin; Hydrogen peroxide; Single-head broccoli-like