title: RAB1A promotes Vaccinia virus replication by facilitating the production of intracellular enveloped virions.
abstract: Vaccinia virus (VACV) is a large double-stranded DNA virus with a complex cytoplasmic replication cycle that exploits numerous cellular proteins. This work characterises the role of a proviral cellular protein, the small GTPase RAB1A, in VACV replication. Using siRNA, we identified RAB1A as required for the production of extracellular enveloped virions (EEVs), but not intracellular mature virions (IMVs). Immunofluorescence and electron microscopy further refined the role of RAB1A as facilitating the wrapping of IMVs to become intracellular enveloped virions (IEVs). This is consistent with the known function of RAB1A in maintenance of ER to Golgi transport. VACV can therefore be added to the growing list of viruses which require RAB1A for optimal replication, highlighting this protein as a broadly proviral host factor.
authors.affiliation: The Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh, Roslin, Midlothian EH25 9RG, Scotland.
authors.forename: Tali
authors.initials: T
authors.lastname: Pechenick Jowers
authors.affiliation: The Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh, Roslin, Midlothian EH25 9RG, Scotland.
authors.forename: Rebecca J
authors.initials: RJ
authors.lastname: Featherstone
authors.affiliation: The Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh, Roslin, Midlothian EH25 9RG, Scotland.
authors.forename: Danielle K
authors.initials: DK
authors.lastname: Reynolds
authors.affiliation: The Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh, Roslin, Midlothian EH25 9RG, Scotland.
authors.forename: Helen K
authors.initials: HK
authors.lastname: Brown
authors.affiliation: Division of Cell Signalling and Immunology, College of Life Sciences, University of Dundee, Dundee DD1 5EH, Scotland.
authors.forename: John
authors.initials: J
authors.lastname: James
authors.affiliation: Division of Cell Signalling and Immunology, College of Life Sciences, University of Dundee, Dundee DD1 5EH, Scotland.
authors.forename: Alan
authors.initials: A
authors.lastname: Prescott
authors.affiliation: The Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh, Roslin, Midlothian EH25 9RG, Scotland.
authors.forename: Ismar R
authors.initials: IR
authors.lastname: Haga
authors.affiliation: The Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh, Roslin, Midlothian EH25 9RG, Scotland. Electronic address: pip.beard@roslin.ed.ac.uk.
authors.forename: Philippa M
authors.initials: PM
authors.lastname: Beard
mesh_terms: D000818:Animals; D002460:Cell Line; D045744:Cell Line, Tumor; D002522:Chlorocebus aethiops; D006801:Humans; D014616:Vaccinia virus / Q000235:genetics / Q000502:physiology*; D010948:Viral Plaque Assay; D014771:Virion / Q000502:physiology*; D019065:Virus Assembly; D014779:Virus Replication / Q000502:physiology*; D020761:rab1 GTP-Binding Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D020761:rab1 GTP-Binding Proteins
keywords: Golgi; Poxvirus; RAB1A; Vaccinia virus