title: Genome characterization of a novel chicken picornavirus distantly related to the members of genus Avihepatovirus with a single 2A protein and a megrivirus-like 3' UTR.
abstract: The members of the genus Avihepatovirus and related picornaviruses ("Aalivius") of ducks, turkey and chickens possess identical 2A peptide composition including three functionally unrelated 2A peptides which is a characteristic genome feature of these monophyletic avian picornaviruses. The complete genome of a novel picornavirus provisionally named Orivirus A1 (KM203656) from a cloacal sample of a 4-week-old diarrheic chicken (Gallus gallus domesticus) distantly related to members of genus Avihepatovirus was characterized. The study strain contains a type-II-like IRES, a single 2A protein of unknown function unrelated to the 2A proteins of avihepatoviruses and a long 3' untranslated region (UTR) with multiple repeated sequence motifs followed by an AUG-rich region. The repeated sequences of the 3' UTR show significant identity to the "Unit A" sequences of the phylogenetically distant megriviruses. The presence of a novel single 2A and the megrivirus-like "Unit A" motifs suggest multiple recombination events in the evolution of this novel picornavirus.
authors.affiliation: Regional Laboratory of Virology, National Reference Laboratory of Gastroenteric Viruses, ÁNTSZ Regional Institute of State Public Health Service, Pécs, Hungary.
authors.forename: Ákos
authors.initials: Á
authors.lastname: Boros
authors.affiliation: Regional Laboratory of Virology, National Reference Laboratory of Gastroenteric Viruses, ÁNTSZ Regional Institute of State Public Health Service, Pécs, Hungary.
authors.forename: Péter
authors.initials: P
authors.lastname: Pankovics
authors.affiliation: Regional Laboratory of Virology, National Reference Laboratory of Gastroenteric Viruses, ÁNTSZ Regional Institute of State Public Health Service, Pécs, Hungary.
authors.forename: Ádám
authors.initials: Á
authors.lastname: Adonyi
authors.affiliation: Blood Systems Research Institute, San Francisco, CA, USA; University of California, San Francisco, CA, USA.
authors.forename: Tung Gia
authors.initials: TG
authors.lastname: Phan
authors.affiliation: Blood Systems Research Institute, San Francisco, CA, USA; University of California, San Francisco, CA, USA.
authors.forename: Eric
authors.initials: E
authors.lastname: Delwart
authors.affiliation: Regional Laboratory of Virology, National Reference Laboratory of Gastroenteric Viruses, ÁNTSZ Regional Institute of State Public Health Service, Pécs, Hungary. Electronic address: reuter.gabor@ddr.antsz.hu.
authors.forename: Gábor
authors.initials: G
authors.lastname: Reuter
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics*; D000818:Animals; D002645:Chickens / Q000821:virology; D003546:Cysteine Endopeptidases / Q000235:genetics*; D016679:Genome, Viral / Q000235:genetics*; D009690:Nucleic Acid Conformation; D010849:Picornaviridae / Q000145:classification* / Q000235:genetics*; D010850:Picornaviridae Infections / Q000821:virology*; D012367:RNA, Viral / Q000737:chemistry; D014764:Viral Proteins / Q000235:genetics*
chemical_list: D020413:3' Untranslated Regions; D012367:RNA, Viral; D014764:Viral Proteins; D003546:Cysteine Endopeptidases
keywords: 3′ UTR; Avian; IRES; Pathogen; Picornavirus; Recombination