title: N-Glycosylation of the archaellum filament is not important for archaella assembly and motility, although N-Glycosylation is essential for motility in Sulfolobus acidocaldarius.
abstract: N-Glycosylation is one of the predominant posttranslational modifications, which is found in all three domains of life. N-Glycosylation has been shown to influence many biological aspects of proteins, like protein folding, stability or activity. In this study we demonstrate that the archaellum filament subunit FlaB of Sulfolobus acidocaldarius is N-glycosylated. Each of the six predicted N-Glycosylation sites within FlaB are modified with the attachment of an N-glycan. Although, it has been previously shown that N-Glycosylation is essential for motility in S. acidocaldarius, as defects in the N-Glycosylation process resulted in none or reduced motile cells, strains lacking one to all six N-Glycosylation sites within FlaB still remained motile. Deletion of the first five N-Glycosylation sites in FlaB did not significantly affect the motility, whereas removal of all six N-Glycosylation sites reduced motility by about 40%. Transmission electron microscopy analyses of non glycosylated and glycosylated archaellum filament revealed no structural change in length. Therefore N-Glycosylation does not appear to be important for the stability and assembly of the archaellum filament itself, but plays a role in other parts of the archaellum assembly.
authors.affiliation: Molecular Biology of Archaea, University of Freiburg, Institute of Biology, Microbiology, Schänzlestrasse 1, 79104 Freiburg, Germany; Molecular Biology of Archaea, Max Planck Institute for Terrestrial Microbiology, Karl-von-Frisch-Str. 10, 35043 Marburg, Germany.
authors.forename: Benjamin H
authors.initials: BH
authors.lastname: Meyer
authors.affiliation: Molecular Biology of Archaea, Max Planck Institute for Terrestrial Microbiology, Karl-von-Frisch-Str. 10, 35043 Marburg, Germany.
authors.forename: Anton
authors.initials: A
authors.lastname: Birich
authors.affiliation: Molecular Biology of Archaea, University of Freiburg, Institute of Biology, Microbiology, Schänzlestrasse 1, 79104 Freiburg, Germany; Molecular Biology of Archaea, Max Planck Institute for Terrestrial Microbiology, Karl-von-Frisch-Str. 10, 35043 Marburg, Germany. Electronic address: sonja.albers@biologie.uni-freiburg.de.
authors.forename: Sonja-Verena
authors.initials: SV
authors.lastname: Albers
mesh_terms: D000595:Amino Acid Sequence; D019843:Archaeal Proteins / Q000378:metabolism*; D005408:Flagellin / Q000378:metabolism*; D006031:Glycosylation; D046529:Microscopy, Electron, Transmission; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D016945:Sulfolobus acidocaldarius / Q000502:physiology*
chemical_list: D019843:Archaeal Proteins; D005408:Flagellin; C416532:flaB flagellin
keywords: Archaea; Archaellum; Flagellum; N-Glycosylation; S-layer; Sulfolobus