title: Expanding the linear dynamic range for quantitative liquid chromatography-high resolution mass spectrometry utilizing natural isotopologue signals.
abstract: The linear dynamic range (LDR) for quantitative liquid chromatography-mass spectrometry can be extended until ionization saturation is reached by using a number of target isotopologue ions in addition to the normally used target ion that provides the highest sensitivity. Less abundant isotopologue ions extend the LDR: the lower ion abundance decreases the probability of ion detector saturation. Effectively the sensitivity decreases and the upper limit of the LDR increases. We show in this paper that the technique is particularly powerful with a high resolution time of flight mass spectrometer because the data for all ions are automatically acquired, and we demonstrated this for four small organic molecules; the upper limits of LDRs increased by 25-50 times.
authors.affiliation: Senomyx Inc. 4767 Nexus Centre Dr., San Diego, CA 92121, USA. Electronic address: Hanghui.Liu@senomyx.com.
authors.forename: Hanghui
authors.initials: H
authors.lastname: Liu
authors.affiliation: Senomyx Inc. 4767 Nexus Centre Dr., San Diego, CA 92121, USA.
authors.forename: Lily
authors.initials: L
authors.lastname: Lam
authors.affiliation: Senomyx Inc. 4767 Nexus Centre Dr., San Diego, CA 92121, USA.
authors.forename: Lin
authors.initials: L
authors.lastname: Yan
authors.affiliation: Senomyx Inc. 4767 Nexus Centre Dr., San Diego, CA 92121, USA.
authors.forename: Bert
authors.initials: B
authors.lastname: Chi
authors.affiliation: Department of Chemistry and Biochemistry, The University of Texas at Arlington, Arlington, TX 76019-0065, USA. Electronic address: Dasgupta@uta.edu.
authors.forename: Purnendu K
authors.initials: PK
authors.lastname: Dasgupta
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D007477:Ions / Q000032:analysis*; D016014:Linear Models; D013058:Mass Spectrometry / Q000379:methods; D008862:Microsomes, Liver / Q000737:chemistry
chemical_list: D007477:Ions
keywords: Isotopologue; Linear dynamic range; Mass spectrometry; Quantitative; Signal saturation