title: Effects of ethanol exposure and withdrawal on dendritic morphology and spine density in the nucleus accumbens core and shell.
abstract: Exposure to drugs of abuse can result in profound structural modifications on neurons in circuits involved in addiction that may contribute to drug dependence, withdrawal and related processes. Structural alterations on medium spiny neurons (MSNs) of the nucleus accumbens (NAc) have been observed following exposure to and withdrawal from a variety of drugs; however, relatively little is known about the effects of alcohol exposure and withdrawal on structural alterations of NAc MSNs. In the present study male rats were chronically exposed to vaporized ethanol for 10 days and underwent 1 or 7 days of withdrawal after which the brains were processed for Golgi-Cox staining and analysis of dendritic length, branching and spine density. MSNs of the NAc shell and core underwent different patterns of changes following ethanol exposure and withdrawal. At 1 day of withdrawal there were modest reductions in the dendritic length and branching of MSNs in both the core and the shell compared to control animals exposed only to air. At 7 days of withdrawal the length and branching of shell MSNs was reduced, whereas the length and branching of core MSNs were increased relative to the shell. The density of mature spines was increased in the core at 1 day of withdrawal, whereas the density of less mature spines was increased in both regions at 7 days of withdrawal. Collectively, these observations indicate that MSNs of the NAc core and shell undergo distinct patterns of structural modifications following ethanol exposure and withdrawal suggesting that modifications in dendritic structure in these regions may contribute differentially to ethanol withdrawal.
authors.affiliation: Department of Psychology, University of New Mexico, Albuquerque, NM 87131, USA.
authors.forename: Veronica L
authors.initials: VL
authors.lastname: Peterson
authors.affiliation: Department of Physiology and Pharmacology, Wake Forest School of Medicine, Winston-Salem, NC 27157, USA.
authors.forename: Brian A
authors.initials: BA
authors.lastname: McCool
authors.affiliation: Department of Psychology, University of New Mexico, Albuquerque, NM 87131, USA; Department of Neurosciences, University of New Mexico, Albuquerque, NM 87131, USA. Electronic address: dahamilt@unm.edu.
authors.forename: Derek A
authors.initials: DA
authors.lastname: Hamilton
mesh_terms: D000437:Alcoholism / Q000473:pathology*; D000818:Animals; D049229:Dendritic Spines / Q000187:drug effects* / Q000648:ultrastructure*; D004195:Disease Models, Animal; D000431:Ethanol / Q000633:toxicity; D008297:Male; D009714:Nucleus Accumbens / Q000187:drug effects* / Q000648:ultrastructure; D051381:Rats; D017207:Rats, Sprague-Dawley; D013375:Substance Withdrawal Syndrome / Q000473:pathology*
chemical_list: D000431:Ethanol
keywords: Addiction; Alcohol; Golgi; Medium spiny neuron; Structural plasticity