title: PDMS substrate stiffness affects the morphology and growth profiles of cancerous prostate and melanoma cells.
abstract: A deep understanding of the interaction between cancerous cells and surfaces is particularly important for the design of lab-on-chip devices involving the use of polydimethylsiloxane (PDMS). In our studies, the effect of PDMS substrate stiffness on mechanical properties of cancerous cells was investigated in conditions where the PDMS substrate is not covered with any of extracellular matrix proteins. Two human prostate cancer (Du145 and PC-3) and two melanoma (WM115 and WM266-4) cell lines were cultured on two groups of PDMS substrates that were characterized by distinct stiffness, i.e. 0.75 ± 0.06 MPa and 2.92 ± 0.12 MPa. The results showed the strong effect on cellular behavior and morphology. The detailed analysis of chemical and physical properties of substrates revealed that cellular behavior occurs only due to substrate elasticity.
authors.affiliation: The Henryk Niewodniczański Institute of Nuclear Physics, Polish Academy of Sciences, Radzikowskiego 152, 31-342 Kraków, Poland.
authors.forename: Szymon
authors.initials: S
authors.lastname: Prauzner-Bechcicki
authors.affiliation: The Marian Smoluchowski Institute of Physics, Jagiellonian University, Reymonta 4, 30-059 Kraków, Poland.
authors.forename: Joanna
authors.initials: J
authors.lastname: Raczkowska
authors.affiliation: The Marian Smoluchowski Institute of Physics, Jagiellonian University, Reymonta 4, 30-059 Kraków, Poland.
authors.forename: Ewelina
authors.initials: E
authors.lastname: Madej
authors.affiliation: The Henryk Niewodniczański Institute of Nuclear Physics, Polish Academy of Sciences, Radzikowskiego 152, 31-342 Kraków, Poland.
authors.forename: Joanna
authors.initials: J
authors.lastname: Pabijan
authors.affiliation: Czech Technical University in Prague, Faculty of Mechanical Engineering, Technicka 4, 16607 Prague, Czech Republic.
authors.forename: Jaroslav
authors.initials: J
authors.lastname: Lukes
authors.affiliation: Czech Technical University in Prague, Faculty of Mechanical Engineering, Technicka 4, 16607 Prague, Czech Republic.
authors.forename: Josef
authors.initials: J
authors.lastname: Sepitka
authors.affiliation: The Marian Smoluchowski Institute of Physics, Jagiellonian University, Reymonta 4, 30-059 Kraków, Poland.
authors.forename: Jakub
authors.initials: J
authors.lastname: Rysz
authors.affiliation: The Marian Smoluchowski Institute of Physics, Jagiellonian University, Reymonta 4, 30-059 Kraków, Poland.
authors.forename: Kamil
authors.initials: K
authors.lastname: Awsiuk
authors.affiliation: Faculty of Physics and Applied Computer Science & Academic Centre for Materials and Nanotechnology, AGH University of Science and Technology, Reymonta 19, 30-049 Kraków, Poland.
authors.forename: Andrzej
authors.initials: A
authors.lastname: Bernasik
authors.affiliation: The Marian Smoluchowski Institute of Physics, Jagiellonian University, Reymonta 4, 30-059 Kraków, Poland.
authors.forename: Andrzej
authors.initials: A
authors.lastname: Budkowski
authors.affiliation: The Henryk Niewodniczański Institute of Nuclear Physics, Polish Academy of Sciences, Radzikowskiego 152, 31-342 Kraków, Poland. Electronic address: Malgorzata.Lekka@ifj.edu.pl.
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Lekka
mesh_terms: D001696:Biomechanical Phenomena; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D004129:Dimethylpolysiloxanes / Q000737:chemistry / Q000494:pharmacology*; D004548:Elasticity; D005340:Fibrinogen / Q000737:chemistry; D006801:Humans; D008297:Male; D055595:Mechanical Phenomena; D008545:Melanoma / Q000473:pathology*; D046208:Microchip Analytical Procedures; D011471:Prostatic Neoplasms / Q000473:pathology*; D013499:Surface Properties
chemical_list: D004129:Dimethylpolysiloxanes; C013830:baysilon; D005340:Fibrinogen
keywords: Cancer cell growth profile; PDMS stiffness; Poly(dimethyl)siloxane (PDMS) surface; Prostate and melanoma