title: Microbubble injection enhances inhibition of low-intensity pulsed ultrasound on debris-induced periprosthetic osteolysis in rabbit model.
abstract: We determined whether the addition of microbubbles enhances the effect of low-intensity pulsed ultrasound (LIPUS) on bone-implant integration in an early-stage osteolysis model. The bone canals were injected with titanium particles before implantation to establish the periprosthetic osteolysis model. Before ultrasonic therapy, the microbubble-enhanced LIPUS group (GTi-Us-Mb) received an intra-articular injection of microbubbles. Biomechanical testing revealed that GTi-Us-Mb had significantly greater fixation strength than the LIPUS group (GTi-Us). Distal periprosthetic bone mineral density was also higher in GTi-Us than in the Ti group (GTi), but no significant increase was detected after administration of microbubbles. Histomorphometric analyses revealed that bone formation around the implant in GTi-Us was enhanced by the addition of microbubbles in GTi-Us-Mb. Taken together, our data indicate that microbubble injection enhances the inhibitory effect of LIPUS on debris-induced osteolysis and further strengthens the mechanical fixation of implants in an early-stage osteolysis model in vivo.
authors.affiliation: Department of Orthopedic Surgery, Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Hu
authors.affiliation: Department of Orthopedic Surgery, Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang, China; Institute of Orthopedic Research, Zhejiang University, Hangzhou, Zhejiang, China.
authors.forename: Xun-Zi
authors.initials: XZ
authors.lastname: Cai
authors.affiliation: Department of Orthopedic Surgery, Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang, China; Institute of Orthopedic Research, Zhejiang University, Hangzhou, Zhejiang, China.
authors.forename: Zhong-Li
authors.initials: ZL
authors.lastname: Shi
authors.affiliation: Department of Orthopedic Surgery, Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang, China.
authors.forename: Yun-Lin
authors.initials: YL
authors.lastname: Chen
authors.affiliation: Department of Orthopedic Surgery, Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang, China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Zhao
authors.affiliation: Department of Orthopedic Surgery, Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang, China.
authors.forename: Han-Xiao
authors.initials: HX
authors.lastname: Zhu
authors.affiliation: Department of Orthopedic Surgery, Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang, China; Institute of Orthopedic Research, Zhejiang University, Hangzhou, Zhejiang, China. Electronic address: jointsahzu@aliyun.com.
authors.forename: Shi-Gui
authors.initials: SG
authors.lastname: Yan
mesh_terms: D000818:Animals; D005547:Foreign Bodies / Q000209:etiology* / Q000628:therapy*; D007270:Injections, Intra-Articular; D007595:Joint Prosthesis / Q000009:adverse effects*; D008297:Male; D045423:Microbubbles / Q000627:therapeutic use*; D010014:Osteolysis / Q000209:etiology* / Q000473:pathology / Q000628:therapy*; D011817:Rabbits; D016896:Treatment Outcome; D014464:Ultrasonic Therapy / Q000379:methods*
keywords: Implant osseo-integration; Low-intensity pulsed ultrasound; Microbubble; Osteolysis