title: Expression of P-gp in acute myeloid leukemia and the reversal function of As2O3on drug resistance.
abstract: To study the expression of P-glycoprotein (P-gp) and the reversal function of As2O3, the active ingredient of arsenic, on drug resistance in acute myeloid leukemia (AML) patients, P-gp and cluster of differentiation 34 (CD34) were examined in primary mononuclear and resistant cells, with or without As2O3. In addition, multidrug resistance gene 1 (MDR1) mRNA expression was investigated in K562/D cells and AML patients. In total, 28.6% of newly-treated (NT) patients and 59.1% of relapsed/refractory (RR) patients were P-gpfunction+, and 31.7% of NT patients and 59.1% of RR patients were CD34+. The positivity rate of P-gpfunctionand CD34+expression in the RR group were significantly higher compared with that in the NT group (P<0.05). In addition, higher CD34+, P-gpexpression+and P-gpfunction+values were observed in older patients compared with younger patients.MDR1expression was downregulated in certain patients following treatment with AS2O3. In the present study, the overexpression of P-gp was the primary cause of drug resistance in the AML patients, andMDR1expression was downregulated by As2O3in primary leukemia and drug-resistant cells.
authors.affiliation: Department of Genetics, China Medical University, Shenyang, Liaoning 110001, P.R. China ; The First Hospital of China Medical University, Shenyang, Liaoning 110001, P.R. China.
authors.forename: Feng
authors.initials: F
authors.lastname: Gao
authors.affiliation: Laboratory Animal Center, China Medical University, Shenyang, Liaoning 110001, P.R. China.
authors.forename: Wanwei
authors.initials: W
authors.lastname: Dong
authors.affiliation: Laboratory Animal Center, China Medical University, Shenyang, Liaoning 110001, P.R. China.
authors.forename: Wei
authors.initials: W
authors.lastname: Yang
authors.affiliation: Laboratory Animal Center, China Medical University, Shenyang, Liaoning 110001, P.R. China.
authors.forename: Jia
authors.initials: J
authors.lastname: Liu
authors.affiliation: Laboratory Animal Center, China Medical University, Shenyang, Liaoning 110001, P.R. China ; Department of Pathology and Pathophysiology Research, China Medical University, Shenyang, Liaoning 110001, P.R. China.
authors.forename: Zhihong
authors.initials: Z
authors.lastname: Zheng
authors.affiliation: Department of Genetics, China Medical University, Shenyang, Liaoning 110001, P.R. China.
authors.forename: Kailai
authors.initials: K
authors.lastname: Sun
keywords: As2O3; MDR1; P-gp; acute myeloid leukemia