title: High throughput and multiplex localization of proteins and cells for in situ micropatterning using pneumatic microfluidics.
abstract: Micropatterning technologies are emerging as an enabling tool for various microfluidic-based applications in life sciences. However, the high throughput and multiplex localization of multiple bio-components in a microfluidic device has not yet been well established. In this paper, we describe a simple and in situ micropatterning method using an integrated microfluidic device with pneumatic microstructures (PμSs) for highly controllable immobilization of both proteins and cells in a high throughput, geometry-dynamic, and multi-patterning way. The precise Pluronic F127 passivation of a microchamber surface except the PμS-blocked regions was performed and characterized, and the spatial dynamics and consistency of both the PμSs and protein/cell micropatterning were optically evaluated and quantitatively demonstrated too. Furthermore, a systematic investigation of PμS-assisted micropatterning in microfluidics was carried out. The feature of high throughput and spatial control of micropatterning can be simply realized by using the well-designed PμS arrays. Meanwhile, the co-micropatterning of different proteins (bovine serum albumin and chicken egg albumin) and cells (human umbilical vein endothelial cells and human hepatocellular carcinoma cells) in a microfluidic device was successfully accomplished with the orderly serial manipulation of PμS groups. We demonstrate that PμS-assisted micropatterning can be applied as a convenient microfluidic component for large-scale and diversified protein/cell patterning and manipulation, which could be useful for cell-based tissue organization, high-throughput imaging, protein-related interactions and immunoassays.
authors.affiliation: Colleges of Science and Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi 712100, China. jywang@nwsuaf.edu.cn.
authors.forename: Jian-Chun
authors.initials: JC
authors.lastname: Wang
authors.forename: Wenming
authors.initials: W
authors.lastname: Liu
authors.forename: Qin
authors.initials: Q
authors.lastname: Tu
authors.forename: Chao
authors.initials: C
authors.lastname: Ma
authors.forename: Lei
authors.initials: L
authors.lastname: Zhao
authors.forename: Yaolei
authors.initials: Y
authors.lastname: Wang
authors.forename: Jia
authors.initials: J
authors.lastname: Ouyang
authors.forename: Long
authors.initials: L
authors.lastname: Pang
authors.forename: Jinyi
authors.initials: J
authors.lastname: Wang
mesh_terms: D006528:Carcinoma, Hepatocellular / Q000737:chemistry*; D002478:Cells, Cultured; D057166:High-Throughput Screening Assays / Q000295:instrumentation*; D061307:Human Umbilical Vein Endothelial Cells / Q000737:chemistry*; D006801:Humans; D007091:Image Processing, Computer-Assisted; D007118:Immunoassay; D008113:Liver Neoplasms / Q000737:chemistry*; D044085:Microfluidics / Q000295:instrumentation*; D011506:Proteins / Q000032:analysis*; D013499:Surface Properties
chemical_list: D011506:Proteins