title: Label-free and enzyme-free sensitive fluorescent detection of human immunodeficiency virus deoxyribonucleic acid based on hybridization chain reaction.
abstract: A label-free and enzyme-free sensitive fluorescent detection of human immunodeficiency virus (HIV) deoxyribonucleic acid (DNA) based on isothermal hybridization chain reaction (HCR) was developed. A G-quadruplex sequence which was incorporated into one of the two hairpin probes was inactive in the absence of target DNA. However, at the presence of target DNA numerous G-quadruplexes along DNA nanowires were self-assembled through HCR. Using N-methyl mesoporphyrin IX (NMM) as the fluorophore, a "turn-on" fluorescent response would be achieved and detected as low as 0.5 nmol L(-1) of HIV DNA. This proposed method was applied to detect HIV DNA in biologic samples with satisfactory results.
authors.affiliation: Ministry of Education Key Laboratory of Analysis and Detection for food safety, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, College of Chemistry, Fuzhou University, Fuzhou 350116, China.
authors.forename: Qingquan
authors.initials: Q
authors.lastname: Guo
authors.affiliation: Ministry of Education Key Laboratory of Analysis and Detection for food safety, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, College of Chemistry, Fuzhou University, Fuzhou 350116, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Ministry of Education Key Laboratory of Analysis and Detection for food safety, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, College of Chemistry, Fuzhou University, Fuzhou 350116, China.
authors.forename: Zhiping
authors.initials: Z
authors.lastname: Song
authors.affiliation: Ministry of Education Key Laboratory of Analysis and Detection for food safety, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, College of Chemistry, Fuzhou University, Fuzhou 350116, China. Electronic address: lqguo@fzu.edu.cn.
authors.forename: Liangqia
authors.initials: L
authors.lastname: Guo
authors.affiliation: Ministry of Education Key Laboratory of Analysis and Detection for food safety, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, College of Chemistry, Fuzhou University, Fuzhou 350116, China.
authors.forename: Fengfu
authors.initials: F
authors.lastname: Fu
authors.affiliation: Ministry of Education Key Laboratory of Analysis and Detection for food safety, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, College of Chemistry, Fuzhou University, Fuzhou 350116, China.
authors.forename: Guonan
authors.initials: G
authors.lastname: Chen
mesh_terms: D004279:DNA, Viral / Q000032:analysis* / Q000235:genetics; D005456:Fluorescent Dyes / Q000737:chemistry; D054856:G-Quadruplexes; D006678:HIV / Q000235:genetics* / Q000302:isolation & purification; D015658:HIV Infections / Q000175:diagnosis / Q000821:virology*; D006801:Humans; D057230:Limit of Detection; D008652:Mesoporphyrins / Q000737:chemistry; D009693:Nucleic Acid Hybridization / Q000379:methods; D013050:Spectrometry, Fluorescence / Q000379:methods
chemical_list: D004279:DNA, Viral; D005456:Fluorescent Dyes; D008652:Mesoporphyrins; C031468:mesoporphyrin IX
keywords: G-quadruplexes; Human immunodeficiency virus; Hybridization chain reaction; N- methyl mesoporphyrin IX