title: The transcriptome of human pluripotent stem cells.
abstract: Human Embryonic Stem Cells (hESCs) are in vitro derivatives of the inner cell mass of the blastocyst and are characterized by an undifferentiated and pluripotent state that can be perpetuated in time, indefinitely. hESCs provide a unique opportunity to both dissect the molecular mechanisms that are predisposed to the maintenance of pluripotency and model the ability to initiate differentiation and cell commitment within the developing embryo. To fully understand these mechanisms, it is necessary to accurately identify the specific transcriptome of hESCs. Many distinct gene annotation methods, such as cDNA and EST sequencing and RNA-Seq, have been used to identify the transcriptome of hESCs. Lately, we developed a new tool (IDP) to integrate the hybrid sequencing data to characterize a more reliable and comprehensive hESC transcriptome with discoveries of many novel transcripts.
authors.affiliation: Department of Internal Medicine, University of Iowa, USA. Electronic address: kinfai-au@uiowa.edu.
authors.forename: Kin Fai
authors.initials: KF
authors.lastname: Au
authors.affiliation: Institute for Stem Cell Biology and Regenerative Medicine, Department of Obstetrics and Gynecology, Stanford University School of Medicine, Stanford, CA 94305, USA.
authors.forename: Vittorio
authors.initials: V
authors.lastname: Sebastiano
mesh_terms: D006801:Humans; D039904:Pluripotent Stem Cells / Q000166:cytology* / Q000378:metabolism*; D059467:Transcriptome