title: A novel method to characterize bacterial communities affected by carbon source and electricity generation in microbial fuel cells using stable isotope probing and Illumina sequencing.
abstract: Stable isotope probing and high throughput sequencing were used to characterize the microbial communities involved in carbon uptake in microbial fuel cells at two levels of electricity generation. With acetate, the dominant phylotypes involved in carbon uptake included Geobacter and Rhodocyclaceae. With glucose, both Enterobacteriaceae and Geobacter were dominant.
authors.affiliation: Department of Civil and Environmental Engineering, Michigan State University, East Lansing, 48824, MI, USA.
authors.forename: Yang
authors.initials: Y
authors.lastname: Song
authors.affiliation: Department of Civil and Environmental Engineering, University of Wisconsin - Milwaukee, Milwaukee, WI 53211, USA.
authors.forename: Li
authors.initials: L
authors.lastname: Xiao
authors.affiliation: Department of Civil and Environmental Engineering, Michigan State University, East Lansing, 48824, MI, USA.
authors.forename: Indumathy
authors.initials: I
authors.lastname: Jayamani
authors.affiliation: Department of Civil and Environmental Engineering, Virginia Polytechnic Institute and State University, Blacksburg, 24061, VA, USA.
authors.forename: Zhen
authors.initials: Z
authors.lastname: He
authors.affiliation: Department of Civil and Environmental Engineering, Michigan State University, East Lansing, 48824, MI, USA. Electronic address: cupplesa@msu.edu.
authors.forename: Alison M
authors.initials: AM
authors.lastname: Cupples
mesh_terms: D001419:Bacteria / Q000737:chemistry / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D015373:Bacterial Typing Techniques; D001674:Bioelectric Energy Sources; D002244:Carbon / Q000032:analysis / Q000378:metabolism*; D004560:Electricity; D004566:Electrodes; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods*; D007553:Isotope Labeling / Q000379:methods*
chemical_list: D002244:Carbon
keywords: Bioenergy; Illumina sequencing; Microbial community; Microbial fuel cells; Stable isotope probing