title: Oral immunization with recombinant hepatitis E virus antigen displayed on the Lactococcus lactis surface enhances ORF2-specific mucosal and systemic immune responses in mice.
abstract: Hepatitis E virus (HEV) as a recognized zoonotic pathogen has posed global burden on public health, which is exacerbated by lack of efficient vaccine. In this study, we constructed a recombinant (inaQ-ORF2 gene fusion) Lactococcus lactis (L. lactis) strain NZ3900 that expresses and displays the hepatitis E virus antigen ORF2 utilizing an ice uncleation protein-based anchor system. After oral vaccination of BALB/c mice, significantly higher levels of ORF2-specific mucosal IgA and serum IgG were detected and cellular immunity was also induced. These findings further support that L. lactis-based HEV antigen vaccines could be used for human and animal protection against infection.
authors.affiliation: Department of Animal Husbandry & Veterinary Medicine, Liaoning Medical University, No. 5-48 Renmin Street, Jinzhou 121001, China; Department of Food Science, Shenyang Agricultural University, No.120 Dongling Road, Shenyang 110161, China.
authors.forename: Shenyang
authors.initials: S
authors.lastname: Gao
authors.affiliation: Animal Quarantine Lab, Inspection & Quarantine Technology Center of Hainan Entry-Exit Inspection & Quarantine Bureau, Haikou 570000, China.
authors.forename: Dandan
authors.initials: D
authors.lastname: Li
authors.affiliation: Department of Animal Husbandry & Veterinary Medicine, Liaoning Medical University, No. 5-48 Renmin Street, Jinzhou 121001, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Animal Husbandry & Veterinary Medicine, Liaoning Medical University, No. 5-48 Renmin Street, Jinzhou 121001, China; Department of Food Science and Engineering, Liaoning Medical University, No. 5-48 Renmin Street, Jinzhou 121001, China. Electronic address: 55053287@qq.com.
authors.forename: Enhui
authors.initials: E
authors.lastname: Zha
authors.affiliation: Department of Animal Husbandry & Veterinary Medicine, Liaoning Medical University, No. 5-48 Renmin Street, Jinzhou 121001, China.
authors.forename: Tiezhong
authors.initials: T
authors.lastname: Zhou
authors.affiliation: Department of Food Science, Shenyang Agricultural University, No.120 Dongling Road, Shenyang 110161, China; Department of Animal Husbandry & Veterinary Medicine, Liaoning Medical University, No. 5-48 Renmin Street, Jinzhou 121001, China.
authors.forename: Xiqing
authors.initials: X
authors.lastname: Yue
mesh_terms: D000284:Administration, Oral; D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics; D005260:Female; D016751:Hepatitis E / Q000276:immunology / Q000517:prevention & control*; D016752:Hepatitis E virus / Q000276:immunology*; D006801:Humans; D007111:Immunity, Cellular; D018928:Immunity, Mucosal; D007114:Immunization; D007070:Immunoglobulin A / Q000097:blood; D007074:Immunoglobulin G / Q000097:blood; D013294:Lactococcus lactis / Q000235:genetics; D051379:Mice; D008807:Mice, Inbred BALB C; D011993:Recombinant Fusion Proteins / Q000235:genetics; D014761:Viral Hepatitis Vaccines / Q000008:administration & dosage / Q000276:immunology*; D014764:Viral Proteins / Q000235:genetics / Q000276:immunology*; D015047:Zoonoses / Q000276:immunology / Q000517:prevention & control*
chemical_list: D001425:Bacterial Outer Membrane Proteins; D007070:Immunoglobulin A; D007074:Immunoglobulin G; C400971:ORF2 protein, Hepatitis E virus; D011993:Recombinant Fusion Proteins; D014761:Viral Hepatitis Vaccines; D014764:Viral Proteins; C063113:ice nucleation protein
keywords: Hepatitis E virus; Ice nucleation protein; Lactococcus lactis; Oral immunization