title: Quantitative glycomics monitoring of induced pluripotent- and embryonic stem cells during neuronal differentiation.
abstract: Alterations in the structure of cell surface glycoforms occurring during the stages of stem cell differentiation remain unclear. We describe a rapid glycoblotting-based cellular glycomics method for quantitatively evaluating changes in glycoform expression and structure during neuronal differentiation of murine induced pluripotent stem cells (iPSCs) and embryonic stem cells (ESCs). Our results show that changes in the expression of cellular N-glycans are comparable during the differentiation of iPSCs and ESCs. The expression of bisect-type N-glycans was significantly up-regulated in neurons that differentiated from both iPSCs and ESCs. From a glycobiological standpoint, iPSCs are an alternative neural cell source in addition to ESCs.
authors.affiliation: Department of Orthopedic Surgery, Hokkaido University School of Medicine, Japan.
authors.forename: Michiyo
authors.initials: M
authors.lastname: Terashima
authors.affiliation: Field of Drug Discovery Research, Faculty of Advanced Life Science and Graduate School of Life Science, Hokkaido University, Japan.
authors.forename: Maho
authors.initials: M
authors.lastname: Amano
authors.affiliation: Department of Orthopedic Surgery, Hokkaido University School of Medicine, Japan.
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Onodera
authors.affiliation: Field of Drug Discovery Research, Faculty of Advanced Life Science and Graduate School of Life Science, Hokkaido University, Japan.
authors.forename: Shin-Ichiro
authors.initials: S
authors.lastname: Nishimura
authors.affiliation: Department of Orthopedic Surgery, Hokkaido University School of Medicine, Japan. Electronic address: niwasaki@med.hokudai.ac.jp.
authors.forename: Norimasa
authors.initials: N
authors.lastname: Iwasaki
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology / Q000378:metabolism; D053595:Embryonic Stem Cells / Q000166:cytology / Q000378:metabolism*; D054794:Glycomics; D057026:Induced Pluripotent Stem Cells / Q000166:cytology / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D017351:N-Acetylglucosaminyltransferases / Q000235:genetics / Q000378:metabolism; D064231:Nestin / Q000235:genetics / Q000378:metabolism; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D011134:Polysaccharides / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D064231:Nestin; D011134:Polysaccharides; D012333:RNA, Messenger; D017351:N-Acetylglucosaminyltransferases; C054599:beta-1,4-mannosyl-glycoprotein beta-1,4-N-acetylglucosaminyltransferase