title: Optimized protocol for the radioiodination of hydrazone-type polymer drug delivery systems.
abstract: Hydrazone conjugates of polymers with doxorubicin represent a very promising tool for cancer chemotherapy. However, these conjugates are very difficult to radiolabel with iodine radionuclides, which possess otherwise very advantageous nuclear properties to, e.g., follow biodistribution. In this study, we developed a robust protocol for the high-yield radioiodination of hydrazone-type drug delivery systems with doxorubicin. In particular, it is crucial that the polymer radioiodination step be performed before the deprotection of the hydrazide and doxorubicin binding.
authors.affiliation: Institute of Macromolecular Chemistry AS CR, v.v.i., Heyrovsky Sq. 2, 162 06 Prague 6, Czech Republic.
authors.forename: Ondřej
authors.initials: O
authors.lastname: Sedláček
authors.affiliation: Institute of Macromolecular Chemistry AS CR, v.v.i., Heyrovsky Sq. 2, 162 06 Prague 6, Czech Republic.
authors.forename: Jan
authors.initials: J
authors.lastname: Kučka
authors.affiliation: Institute of Macromolecular Chemistry AS CR, v.v.i., Heyrovsky Sq. 2, 162 06 Prague 6, Czech Republic. Electronic address: mhruby@centrum.cz.
authors.forename: Martin
authors.initials: M
authors.lastname: Hrubý
keywords: Doxorubicin; Hydrazone; Polymer conjugate; Radioiodination; Radiolabeling; poly[N-(2-hydroxypropyl)methacrylamide]