title: Surface-enhanced Raman spectroscopy detection of dopamine by DNA Targeting amplification assay in Parkisons's model.
abstract: Dopamine is a potent neuromodulator in the brain that influences a variety of motivated behaviors and is involved in several neurologic diseases. We evaluated a bio-barcode amplification assay for its ability to detect dopamine in a mouse model with and without prior administration of the neurotoxin 1-methyl-4-phenyl-1, 2, 3, 6-tetrahydropyridine (MPTP). Our approach uses a combination of DNA barcodes and bead-based immunoassays for detecting neurotransmitters with surface-enhanced Raman spectroscopy (SERS). This method relies on a gold nanoplate with adsorbed antibodies and gold nanoparticles that are encoded with DNA and antibodies that can sandwich the target protein captured by the nanoparticle-bound antibodies. C57BL/6 mice were infused intranasally with MPTP (25mg/kg/day) over 7 consecutive days. At 7 and 21 days after the last administration of MPTP, dopamine was found by western blot analysis to have decreased in the midbrain by 37.44% and 92.95%, respectively. Furthermore, the Raman intensity of dopamine in the midbrains of MPTP-treated mice decreased by 56.77% and 61.12% on days 7 and 21, respectively. Our results demonstrate that the concentration of dopamine in midbrain and striatum of MPTP-treated mice can be easily detected using the bio-barcode assay, which is a rapid, high-throughput screening tool for detecting neurotransmitters.
authors.affiliation: Division of Food Bioscience, Konkuk University, Chungju-si 380-701, South Korea.
authors.forename: Jeung Hee
authors.initials: JH
authors.lastname: An
authors.affiliation: Department of Biotechnology, Konkuk University, Chungju-si 380-701, South Korea.
authors.forename: Dong-Kug
authors.initials: DK
authors.lastname: Choi
authors.affiliation: Division of Food Bioscience, Konkuk University, Chungju-si 380-701, South Korea; Department of Advanced Materials Engineering, Daejeon University, Daejeon 300-716, South Korea.
authors.forename: Kwon-Jai
authors.initials: KJ
authors.lastname: Lee
authors.affiliation: Department of Chemical & Biomolecular Engineering, Sogang University, Seoul 121-742, South Korea. Electronic address: jwchoi@sogang.ac.kr.
authors.forename: Jeong-Woo
authors.initials: JW
authors.lastname: Choi
mesh_terms: D000818:Animals; D015374:Biosensing Techniques; D003342:Corpus Striatum; D004247:DNA / Q000737:chemistry / Q000235:genetics; D004195:Disease Models, Animal; D004298:Dopamine / Q000235:genetics / Q000302:isolation & purification*; D006046:Gold / Q000737:chemistry; D006801:Humans; D051379:Mice; D021141:Nucleic Acid Amplification Techniques; D010300:Parkinson Disease / Q000175:diagnosis* / Q000235:genetics; D020734:Parkinsonian Disorders / Q000175:diagnosis* / Q000235:genetics; D013059:Spectrum Analysis, Raman
chemical_list: D006046:Gold; D004247:DNA; D004298:Dopamine
keywords: Bio-barcode assay; Dopamine; Gold nanoparticles; Surface-enhanced Raman spectroscopy