title: Quantification of the effect of Lipo-PGE1 on angiogenesis.
abstract: Fifteen rabbits were used to assess the effect of Lipo-PGE1 on neovascularization. Merocel(®) and Alloderm(®) of the same size were implanted separately under the back skin to act as matrices for vessel growth. Lipo-PGE1 was injected intravenously for 2 weeks in an experimental group of eight rabbits, and they were compared with a control group of seven untreated animals. Blood flow was measured using the (99m)TcO4(-) clearance technique. The mean blood clearance halftime (T1/2) and washout radioactivity were measured. Newly formed vessels were counted by CD31. The mean clearance halftime was 4005 ± 2161.3 and 13840 ± 4644.6 s in the experimental and control group, respectively, in the 1 × 2 × 1.5-cm-sized implants (p = 0.0125), and 1560 ± 1174.7 and 3405 ± 807.03 s, respectively, in the 2 × 2 × 1.5-cm-sized implants (p = 0.0413). Histological examinations revealed that the mean numbers of newly formed vessels in the experimental and control groups were 11 ± 1.58 and 7.8 ± 1.71, respectively, in the 1 × 2 × 1.5-cm-sized implants (p = 0.0501), and 20.19 ± 12.47 and 12.33 ± 3.25, respectively, in the 2 × 2 × 1.5-cm-sized implants (p = 0.02679). Lipo-PGE1 was found to be effective in promoting angiogenesis in a rabbit matrix model.
authors.affiliation: Department of Plastic and Reconstructive Surgery, DongGuk University Graduate School of Medicine, Seoul, South Korea. Electronic address: surakeo@yahoo.com.
authors.forename: SuRak
authors.initials: S
authors.lastname: Eo
authors.affiliation: Department of Plastic and Reconstructive Surgery, DongGuk University Graduate School of Medicine, Seoul, South Korea.
authors.forename: Chan
authors.initials: C
authors.lastname: Kwon
authors.affiliation: Department of Plastic and Reconstructive Surgery, DongGuk University Graduate School of Medicine, Seoul, South Korea.
authors.forename: HyunJic
authors.initials: H
authors.lastname: Lee
authors.affiliation: Department of Plastic and Reconstructive Surgery, DongGuk University Graduate School of Medicine, Seoul, South Korea.
authors.forename: SangHun
authors.initials: S
authors.lastname: Cho
authors.affiliation: Department of Orthopaedic Surgery, DongGuk University Graduate School of Medicine, Seoul, South Korea.
authors.forename: JinYoung
authors.initials: J
authors.lastname: Kim
authors.affiliation: Department of Orthopaedic Surgery, Seoul National University Hospital, Seoul, South Korea.
authors.forename: GooHyun
authors.initials: G
authors.lastname: Baek
authors.affiliation: Department of Nuclear Medicine, DongGuk University Graduate School of Medicine, Seoul, South Korea.
authors.forename: JeongSeok
authors.initials: J
authors.lastname: Yeo
authors.affiliation: Department of Clinical Trial Test, DongGuk University Graduate School of Medicine, Seoul, South Korea.
authors.forename: ChiYeon
authors.initials: C
authors.lastname: Lim
mesh_terms: D000527:Alprostadil / Q000494:pharmacology*; D000818:Animals; D001707:Biopsy, Needle; D003094:Collagen; D004195:Disease Models, Animal; D005557:Formaldehyde; D007150:Immunohistochemistry; D007275:Injections, Intravenous; D018919:Neovascularization, Physiologic / Q000187:drug effects*; D011142:Polyvinyl Alcohol; D019736:Prostheses and Implants; D011817:Rabbits; D011897:Random Allocation; D012016:Reference Values; D012680:Sensitivity and Specificity; D012867:Skin / Q000098:blood supply* / Q000473:pathology; D014665:Vasodilator Agents / Q000494:pharmacology*
chemical_list: C440568:Alloderm; D014665:Vasodilator Agents; D005557:Formaldehyde; C027490:polyvinyl alcohol formaldehyde foam; D011142:Polyvinyl Alcohol; D003094:Collagen; D000527:Alprostadil
keywords: Angiogenesis-inducing agents; Lipo-PGE1; Prostaglandin E1; Vasodilators