title: The promoter of the white spot syndrome virus immediate-early gene WSSV108 is activated by the cellular KLF transcription factor.
abstract: A series of deletion and mutation assays of the white spot syndrome virus (WSSV) immediate-early gene WSSV108 promoter showed that a Krüppel-like factor (KLF) binding site located from -504 to -495 (relative to the transcription start site) is important for the overall level of WSSV108 promoter activity. Electrophoretic mobility shift assays further showed that overexpressed recombinant Penaeus monodon KLF (rPmKLF) formed a specific protein-DNA complex with the (32)P-labeled KLF binding site of the WSSV108 promoter, and that higher levels of Litopenaeus vannamei KLF (LvKLF) were expressed in WSSV-infected shrimp. A transactivation assay indicated that the WSSV108 promoter was strongly activated by rPmKLF in a dose-dependent manner. Lastly, we found that specific silencing of LvKLF expression in vivo by dsRNA injection dramatically reduced both WSSV108 expression and WSSV replication. We conclude that shrimp KLF is important for WSSV genome replication and gene expression, and that it binds to the WSSV108 promoter to enhance the expression of this immediate-early gene.
authors.affiliation: Department of Earth and Life Science, College of Science, University of Taipei, Taipei 100, Taiwan.
authors.forename: Wang-Jing
authors.initials: WJ
authors.lastname: Liu
authors.affiliation: Institute of Bioinformatics and Biosignal Transduction, College of Bioscience and Biotechnology, National Cheng Kung University, Tainan 701, Taiwan.
authors.forename: Chu-Fang
authors.initials: CF
authors.lastname: Lo
authors.affiliation: Department of Life Science, College of Life Science, National Taiwan University, Taipei 106, Taiwan.
authors.forename: Guang-Hsiung
authors.initials: GH
authors.lastname: Kou
authors.affiliation: Institute of Marine Biology, College of Life Sciences, National Taiwan Ocean University, Keelung 202, Taiwan.
authors.forename: Jiann-Horng
authors.initials: JH
authors.lastname: Leu
authors.affiliation: Department of Food Science, College of Science and Engineering, National Quemoy University, Kinmen 892, Taiwan.
authors.forename: Ying-Jang
authors.initials: YJ
authors.lastname: Lai
authors.affiliation: Department of Biochemical Science and Technology, College of Life Science, National Taiwan University, Taipei 106, Taiwan.
authors.forename: Li-Kwan
authors.initials: LK
authors.lastname: Chang
authors.affiliation: Department of Molecular Biotechnology, College of Biotechnology and Bioresources, Da-Yeh University, Changhua 515, Taiwan. Electronic address: yschang@mail.dyu.edu.tw.
authors.forename: Yun-Shiang
authors.initials: YS
authors.lastname: Chang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D060829:Arthropod Proteins / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D015153:Blotting, Western; D024202:Electrophoretic Mobility Shift Assay; D015967:Gene Expression Regulation, Viral; D017781:Genes, Immediate-Early / Q000235:genetics*; D054884:Host-Pathogen Interactions / Q000235:genetics; D017874:Immediate-Early Proteins; D051741:Kruppel-Like Transcription Factors / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D033561:Penaeidae / Q000235:genetics / Q000378:metabolism / Q000821:virology; D011401:Promoter Regions, Genetic / Q000235:genetics*; D011485:Protein Binding; D034622:RNA Interference; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015533:Transcriptional Activation; D014764:Viral Proteins / Q000235:genetics* / Q000378:metabolism; D014779:Virus Replication / Q000235:genetics; D046848:White spot syndrome virus 1 / Q000235:genetics* / Q000378:metabolism / Q000502:physiology
chemical_list: D060829:Arthropod Proteins; D017874:Immediate-Early Proteins; D051741:Kruppel-Like Transcription Factors; D014764:Viral Proteins; C000600525:WSSV108 protein, white spot syndrome virus
keywords: Immediate-early gene; Krüppel-like factor; Litopenaeus vannamei; Penaeus monodon; WSSV; White spot syndrome virus