title: Recombinant expression and characterization of a serine protease inhibitor (Lvserpin7) from the Pacific white shrimp, Litopenaeus vannamei.
abstract: Serine protease inhibitors (serpins) are widely known to its inhibitory role on proteases involved in the immune responses. Herein, a novel serine protease inhibitor (Lvserpin7), encoding for 411 amino acids with calculated molecular mass of 46.29 kDa and isoelectric point of 6.98 was characterized from the Pacific white shrimp Litopenaeus vannamei. Lvserpin7 shared 92.9% identities to Penaeus monodon serpin7. Among the tested tissues, Lvserpin7 was mainly expressed in hemocytes and gill. The expression profiles analysis indicated that Lvserpin7 was significantly up-regulated in the early stage upon Vibrio anguillarum, Micrococcus lysodeikticus or White Spot Syndrome Virus (WSSV) infection. Fusion protein expression was induced by IPTG, and the purified recombinant Lvserpin7 protein (rLvserpin7) binds to both the Gram-positive and Gram-negative bacteria. Also rLvserpin7 exhibited inhibitory activity against the proteases secreted by Bacillus subtilis. Moreover, rLvserpin7 showed inhibition role on prophenoloxidase activation. To recap, we proposed that Lvserpin7 was implicated in the shrimp immunity via the inhibition of bacterial proteases and proteases involved in prophenoloxidase system.
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Shaanxi Yangling 712100, China.
authors.forename: Yongjie
authors.initials: Y
authors.lastname: Liu
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Shaanxi Yangling 712100, China.
authors.forename: Fujun
authors.initials: F
authors.lastname: Hou
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Shaanxi Yangling 712100, China.
authors.forename: Xianzong
authors.initials: X
authors.lastname: Wang
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Shaanxi Yangling 712100, China. Electronic address: liuxiaolin@nwsuaf.edu.cn.
authors.forename: Xiaolin
authors.initials: X
authors.lastname: Liu
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D060829:Arthropod Proteins / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D004156:Catechol Oxidase / Q000378:metabolism; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics / Q000378:metabolism; D004792:Enzyme Precursors / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics; D008837:Micrococcus / Q000276:immunology; D008969:Molecular Sequence Data; D009928:Organ Specificity; D033561:Penaeidae / Q000235:genetics* / Q000276:immunology* / Q000382:microbiology / Q000821:virology; D010802:Phylogeny; D016415:Sequence Alignment; D015842:Serine Proteinase Inhibitors / Q000235:genetics* / Q000378:metabolism*; D014162:Transfection; D014733:Vibrio / Q000276:immunology; D046848:White spot syndrome virus 1 / Q000276:immunology
chemical_list: D060829:Arthropod Proteins; D018076:DNA, Complementary; D004792:Enzyme Precursors; D015842:Serine Proteinase Inhibitors; C038451:pro-phenoloxidase; D004156:Catechol Oxidase
keywords: Immunity; Litopenaeus vannamei; Prophenoloxidase; Proteases; Serpin7