title: Identification of a new tospovirus causing necrotic ringspot on tomato in China.
abstract: BACKGROUND
  Emerging tospoviruses cause significant yield losses and quality reduction in vegetables, ornamentals, and legumes throughout the world. So far, eight tospoviruses were reported in China. Tomato fruits displaying necrotic and concentric ringspot symptoms were found in Guizhou province of southwest China.
  
  
  RESULTS
  ELISA experiments showed that crude saps of the diseased tomato fruit samples reacted with antiserum against Tomato zonate spot virus (TZSV). Electron microscopy detected presence of quasi-spherical, enveloped particles of 80-100 nm in such saps. The putative virus isolate was designated 2009-GZT. Mechanical back-inoculation showed that 2009-GZT could infect systemically some solanaceous crop and non-crop plants including Capiscum annuum, Datura stramonium, Nicotiana benthamiana, N. rustica, N. tabacum and Solanum lycopersicum. The 3012 nt full-length sequence of 2009-GZT S RNA shared 68.2% nt identity with that of Calla lily chlorotic spot virus (CCSV), the highest among all compared viruses. This RNA was predicted to encode a non-structural protein (NSs) (459 aa, 51.7 kDa) and a nucleocapsid protein (N) (278 aa, 30.3 kDa). The N protein shared 85.8% amino acid identity with that of CCSV. The NSs protein shared 82.7% amino acid identity with that of Tomato zonate spot virus(TZSV).
  
  
  CONCLUSIONS
  Our results indicate that the isolate 2009-GZT is a new species of Tospovirus, which is named Tomato necrotic spot virus (TNSV). This finding suggests that a detailed survey in China is warranted to further understand the occurrence and distribution of tospoviruses.
authors.affiliation: Yunnan Key Laboratory of Agricultural Biotechnology, Key Lab of Southwestern Crop Gene Resources and Germplasm Innovation, Biotechnology and Germplasm Resources Institute, Ministry of Agriculture, Yunnan Academy of Agricultural Sciences, Kunming, 650223, China. yinyy0324@163.com.|Institute of Alpine Economic Plants, Yunnan Academy of Agricultural Sciences, Lijiang, 674100, China. yinyy0324@163.com.
authors.forename: Yueyan
authors.initials: Y
authors.lastname: Yin
authors.affiliation: Yunnan Key Laboratory of Agricultural Biotechnology, Key Lab of Southwestern Crop Gene Resources and Germplasm Innovation, Biotechnology and Germplasm Resources Institute, Ministry of Agriculture, Yunnan Academy of Agricultural Sciences, Kunming, 650223, China. zhengkuanyu@126.com.
authors.forename: Kuanyu
authors.initials: K
authors.lastname: Zheng
authors.affiliation: Yunnan Key Laboratory of Agricultural Biotechnology, Key Lab of Southwestern Crop Gene Resources and Germplasm Innovation, Biotechnology and Germplasm Resources Institute, Ministry of Agriculture, Yunnan Academy of Agricultural Sciences, Kunming, 650223, China. dongjhn@126.com.
authors.forename: Jiahong
authors.initials: J
authors.lastname: Dong
authors.affiliation: Yunnan Key Laboratory of Agricultural Biotechnology, Key Lab of Southwestern Crop Gene Resources and Germplasm Innovation, Biotechnology and Germplasm Resources Institute, Ministry of Agriculture, Yunnan Academy of Agricultural Sciences, Kunming, 650223, China. 324474381@qq.com.
authors.forename: Qi
authors.initials: Q
authors.lastname: Fang
authors.affiliation: Institute of Plant Protection, Guizhou Academy of Agricultural Sciences, Guiyang, 550006, China. gzwusp@126.com.
authors.forename: Shiping
authors.initials: S
authors.lastname: Wu
authors.affiliation: Institute of Plant Protection, Guizhou Academy of Agricultural Sciences, Guiyang, 550006, China. wwllss520@163.com.
authors.forename: Lishuang
authors.initials: L
authors.lastname: Wang
authors.affiliation: Yunnan Key Laboratory of Agricultural Biotechnology, Key Lab of Southwestern Crop Gene Resources and Germplasm Innovation, Biotechnology and Germplasm Resources Institute, Ministry of Agriculture, Yunnan Academy of Agricultural Sciences, Kunming, 650223, China. zhongkai99@sina.com.
authors.forename: Zhongkai
authors.initials: Z
authors.lastname: Zhang
mesh_terms: D002681:China; D016000:Cluster Analysis; D016679:Genome, Viral; D018551:Solanum lycopersicum / Q000821:virology*; D046529:Microscopy, Electron, Transmission; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology*; D012367:RNA, Viral / Q000235:genetics; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D017865:Tospovirus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000648:ultrastructure; D014764:Viral Proteins / Q000235:genetics; D014771:Virion / Q000648:ultrastructure
chemical_list: D012367:RNA, Viral; D014764:Viral Proteins