title: Unraveling the effects of laccase treatment on enzymatic hydrolysis of steam-exploded wheat straw.
abstract: Laccase enzymes are promising detoxifying agents during lignocellulosic bioethanol production from wheat straw. However, they affect the enzymatic hydrolysis of this material by lowering the glucose recovery yields. This work aimed at explaining the negative effects of laccase on enzymatic hydrolysis. Relative glucose recovery in presence of laccase (10IU/g substrate) with model cellulosic substrate (Sigmacell) at 10% (w/v) was almost 10% points lower (P<0.01) than in the absence of laccase. This fact could be due to an increase in the competition of cellulose binding sites between the enzymes and a slight inhibition of β-glucosidase activity. However, enzymatic hydrolysis and infrared spectra of laccase-treated and untreated wheat straw filtered pretreated residue (WS-FPR), revealed that a grafting process of phenoxy radicals onto the lignin fiber could be the cause of diminished accessibility of cellulases to cellulose in pretreated wheat straw.
authors.affiliation: IMDEA Energy Institute, Biotechnology Processes for Energy Production Unit, 28935 Móstoles, Spain.
authors.forename: Alfredo
authors.initials: A
authors.lastname: Oliva-Taravilla
authors.affiliation: IMDEA Energy Institute, Biotechnology Processes for Energy Production Unit, 28935 Móstoles, Spain.
authors.forename: Antonio D
authors.initials: AD
authors.lastname: Moreno
authors.affiliation: IMDEA Energy Institute, Biotechnology Processes for Energy Production Unit, 28935 Móstoles, Spain. Electronic address: marie.demuez@imdea.org.
authors.forename: Marie
authors.initials: M
authors.lastname: Demuez
authors.affiliation: INIA-CIFOR, Forestry Products Department, Cellulose and Paper Laboratories, 28040 Madrid, Spain.
authors.forename: David
authors.initials: D
authors.lastname: Ibarra
authors.affiliation: IMDEA Energy Institute, Biotechnology Processes for Energy Production Unit, 28935 Móstoles, Spain.
authors.forename: Elia
authors.initials: E
authors.lastname: Tomás-Pejó
authors.affiliation: IMDEA Energy Institute, Biotechnology Processes for Energy Production Unit, 28935 Móstoles, Spain.
authors.forename: Cristina
authors.initials: C
authors.lastname: González-Fernández
authors.affiliation: IMDEA Energy Institute, Biotechnology Processes for Energy Production Unit, 28935 Móstoles, Spain; CIEMAT, Renewable Energy Division, Biofuels Unit, 28040 Madrid, Spain.
authors.forename: Mercedes
authors.initials: M
authors.lastname: Ballesteros
mesh_terms: D001665:Binding Sites; D001667:Binding, Competitive; D001709:Biotechnology / Q000379:methods*; D044602:Cellulases / Q000378:metabolism; D002482:Cellulose / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D006868:Hydrolysis; D042845:Laccase / Q000737:chemistry* / Q000378:metabolism; D008031:Lignin / Q000737:chemistry / Q000378:metabolism; D010636:Phenols / Q000737:chemistry; D018520:Plant Shoots / Q000737:chemistry / Q000378:metabolism; D011092:Polyethylene Glycols / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared; D013227:Steam; D014908:Triticum / Q000737:chemistry* / Q000378:metabolism
chemical_list: D010636:Phenols; D013227:Steam; C042329:phenoxy radical; D011092:Polyethylene Glycols; D002482:Cellulose; D008031:Lignin; D042845:Laccase; D044602:Cellulases; D005947:Glucose
keywords: ATR-FTIR; Enzymatic hydrolysis; Grafting; Laccase detoxification; Lignocellulose