title: Relationship between growth factors and its implication in the pathogenesis of leprosy.
abstract: Leprosy is a chronic infectious disease caused by Mycobacterium leprae which affects the skin and peripheral nervous system. The immune response of the host determines the clinical course of the disease. The tuberculoid form is the result of high cell-mediated immunity characterized by a Th1 response, whereas the lepromatous form is characterized by low cell-mediated immunity and a Th2 humoral response. The neural damage established produces marked changes in the expression of growth factors such as nerve growth factor (NGF) and its receptors (NGF-R). The expression of NGF, associated with the expression of Th1 and Th2 cytokines, might be involved in the tissue damage caused by the bacillus. Therefore, the objective of this study was to correlate the immunoexpression patterns of NGF and NGF-R in the different clinical forms of leprosy, and to associate the findings with the in situ expression of TGF-β and clinical classification of the disease. TGF-β, NGF and NGF-R immunoexpression was analyzed by immunohistochemistry in paraffin-embedded material. Most patients were males with a mean age of 40.7 years. TGF-β levels were significantly higher in the lepromatous forms. No significant difference in the immunoexpression of NGF or NGF-R was observed between the clinical forms, but expression tended to be higher at the lepromatous pole. There was a significant positive correlation between NGF and NGF-R in the different clinical forms of leprosy. A significant positive correlation was observed between NGF, NGF-R and TGF-β. It can be concluded that, even existing evidence on the role of these molecules in the clinical spectrum of leprosy.
authors.affiliation: Nucleo de Medicina Tropical, Universidade Federal do Para, Belem, PA, Brazil.
authors.forename: Tinara Leila de Souza
authors.initials: TL
authors.lastname: Aarão
authors.affiliation: Departamento de Patologia, Centro de Ciencias Biologicas e da Saude, Universidade do Estado do Para, Belem, PA, Brazil.
authors.forename: Nelma Rosa
authors.initials: NR
authors.lastname: Esteves
authors.affiliation: Departamento de Patologia, Centro de Ciencias Biologicas e da Saude, Universidade do Estado do Para, Belem, PA, Brazil.
authors.forename: Natalia
authors.initials: N
authors.lastname: Esteves
authors.affiliation: Departamento de Patologia, Centro de Ciencias Biologicas e da Saude, Universidade do Estado do Para, Belem, PA, Brazil.
authors.forename: Luis Paulo de Miranda
authors.initials: LP
authors.lastname: Soares
authors.affiliation: Nucleo de Medicina Tropical, Universidade Federal do Para, Belem, PA, Brazil.
authors.forename: Denise da Silva
authors.initials: Dda S
authors.lastname: Pinto
authors.affiliation: Nucleo de Medicina Tropical, Universidade Federal do Para, Belem, PA, Brazil.
authors.forename: Hellen Thais
authors.initials: HT
authors.lastname: Fuzii
authors.affiliation: Nucleo de Medicina Tropical, Universidade Federal do Para, Belem, PA, Brazil; Departamento de Patologia, Centro de Ciencias Biologicas e da Saude, Universidade do Estado do Para, Belem, PA, Brazil. Electronic address: juarez@pesquisador.cnpq.br.
authors.forename: Juarez Antônio Simões
authors.initials: JA
authors.lastname: Quaresma
mesh_terms: D016207:Cytokines / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D007918:Leprosy / Q000382:microbiology* / Q000473:pathology*; D009166:Mycobacterium leprae / Q000276:immunology*; D009414:Nerve Growth Factors / Q000096:biosynthesis*; D017475:Receptors, Nerve Growth Factor / Q000096:biosynthesis; D053773:Transforming Growth Factor beta1 / Q000096:biosynthesis
chemical_list: D016207:Cytokines; D009414:Nerve Growth Factors; D017475:Receptors, Nerve Growth Factor; D053773:Transforming Growth Factor beta1
keywords: Cytokines; Immunohistochemistry; Leprosy; Nerve growth factor