title: Lymphocyte inhibition is compromised in mesenchymal stem cells from psoriatic skin.
abstract: BACKGROUND
  Psoriasis is a chronic inflammatory skin disorder associated with a host of immune abnormalities. Mesenchymal stem cells (MSCs) have immunosuppressive properties and, in earlier studies, we found that the bone marrow MSCs of patients with psoriasis exhibit abnormal cytokine secretion. Since MSCs can be isolated from skin, we hypothesized that the biological characteristics of MSCs in psoriatic skin lesions might reflect the pathogenesis of psoriasis.
  
  
  OBJECTIVE
  To investigate the effects of MSCs from psoriatic skin lesions on T-cell proliferation.
  
  
  METHODS
  MSCs obtained from psoriatic skin lesions and healthy human skin were examined by flow cytometry and cell differentiation assays. MSCs were co-cultured with normal peripheral blood T cells to assess changes in T-cell proliferation. Concentrations of interleukin (IL)-6, IL-11, hepatocyte growth factor (HGF), and transforming growth factor (TGF)-β1 in the MSC culture supernatants were measured by enzyme-linked immunosorbent assays.
  
  
  RESULTS
  Surface markers and differentiation capacity were similar in MSCs from both sources. MSCs in psoriatic skin lesions were weaker inhibitors of T-cell proliferation (p<0.05) and exhibited increased secretion of IL-11 and reduced secretion of IL-6 and HGF (p<0.05). Secretion of TGF-β1 was unchanged (p > 0.05).
  
  
  CONCLUSIONS
  This study demonstrated abnormalities in MSCs derived from psoriatic skin lesions. We suggest that the attenuated inhibitory effect on T-cell proliferation might be one of the pathogenic mechanisms of psoriasis.
authors.affiliation: Institute of Dermatology, Taiyuan City Centre Hospital, No. 1 Dong San Dao Xiang, Taiyuan, Shanxi Province 030009, China.
authors.forename: Ruifeng
authors.initials: R
authors.lastname: Liu
authors.affiliation: Department of Dermatology, Qinghai University Affiliated Hospital, Xining, Qinghai Province 810000, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Institute of Dermatology, Taiyuan City Centre Hospital, No. 1 Dong San Dao Xiang, Taiyuan, Shanxi Province 030009, China.
authors.forename: Xincheng
authors.initials: X
authors.lastname: Zhao
authors.affiliation: Institute of Dermatology, Taiyuan City Centre Hospital, No. 1 Dong San Dao Xiang, Taiyuan, Shanxi Province 030009, China.
authors.forename: Yuanwen
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Institute of Dermatology, Taiyuan City Centre Hospital, No. 1 Dong San Dao Xiang, Taiyuan, Shanxi Province 030009, China.
authors.forename: Kaiming
authors.initials: K
authors.lastname: Zhang
mesh_terms: D000293:Adolescent; D000328:Adult; D000818:Animals; D002454:Cell Differentiation; D049109:Cell Proliferation; D018920:Coculture Techniques; D005260:Female; D017228:Hepatocyte Growth Factor / Q000378:metabolism; D006801:Humans; D007108:Immune Tolerance; D017370:Interleukin-11 / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D008297:Male; D059630:Mesenchymal Stem Cells / Q000276:immunology* / Q000378:metabolism*; D051379:Mice; D008875:Middle Aged; D011565:Psoriasis / Q000276:immunology*; D013601:T-Lymphocytes / Q000502:physiology*; D053773:Transforming Growth Factor beta1 / Q000378:metabolism; D055815:Young Adult
chemical_list: D017370:Interleukin-11; D015850:Interleukin-6; D053773:Transforming Growth Factor beta1; D017228:Hepatocyte Growth Factor
keywords: immunosuppression; mesenchymal stem cells; psoriasis; skin lesions